LOCUS       DAPGRN010000001       117847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPGRN010000001 DAPGRN010000000
VERSION     DAPGRN010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.001"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            182..808
                     /locus_tag="RHW33_000001"
     CDS             182..808
                     /locus_tag="RHW33_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV1643561.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /locus_tag="RHW33_000002"
     CDS             829..2931
                     /locus_tag="RHW33_000002"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV1643562.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPRDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2944..3582
                     /locus_tag="RHW33_000003"
     CDS             2944..3582
                     /locus_tag="RHW33_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV1643563.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLVNIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRALAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3646..4314
                     /locus_tag="RHW33_000004"
     CDS             3646..4314
                     /locus_tag="RHW33_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV1643564.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4311..5096
                     /locus_tag="RHW33_000005"
                     /pseudo
     CDS             4311..5096
                     /locus_tag="RHW33_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416185.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            5100..5912
                     /locus_tag="RHW33_000006"
     CDS             5100..5912
                     /locus_tag="RHW33_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643565.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAVQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5924..7528)
                     /locus_tag="RHW33_000007"
     CDS             complement(5924..7528)
                     /locus_tag="RHW33_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV1643566.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARKQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7654..7959)
                     /locus_tag="RHW33_000008"
     CDS             complement(7654..7959)
                     /locus_tag="RHW33_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV1643567.1"
                     /translation="MATLLQLHFSFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="RHW33_000009"
     tRNA            complement(8067..8143)
                     /locus_tag="RHW33_000009"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="RHW33_000010"
     tRNA            complement(8148..8224)
                     /locus_tag="RHW33_000010"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /locus_tag="RHW33_000011"
     CDS             8532..9788
                     /locus_tag="RHW33_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV1643568.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVTTGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSIGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQISAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="RHW33_000012"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="RHW33_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV1643569.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLAAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="RHW33_000013"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="RHW33_000013"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV1643570.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="RHW33_000014"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="RHW33_000014"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV1643571.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /locus_tag="RHW33_000015"
     CDS             complement(13537..14748)
                     /locus_tag="RHW33_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV1643572.1"
                     /translation="MLPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     SNAEVAHSESH"
     gene            14861..15793
                     /locus_tag="RHW33_000016"
     CDS             14861..15793
                     /locus_tag="RHW33_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1643573.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="RHW33_000017"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="RHW33_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV1643574.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="ynhF"
                     /locus_tag="RHW33_000018"
     CDS             17114..17203
                     /gene="ynhF"
                     /locus_tag="RHW33_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV1643575.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18538
                     /locus_tag="RHW33_000019"
     CDS             17369..18538
                     /locus_tag="RHW33_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1643576.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="RHW33_000020"
     CDS             complement(18684..19265)
                     /gene="sodB"
                     /locus_tag="RHW33_000020"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV1643577.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19393..20220)
                     /gene="mepH"
                     /locus_tag="RHW33_000021"
     CDS             complement(19393..20220)
                     /gene="mepH"
                     /locus_tag="RHW33_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV1643578.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20555..20902
                     /gene="grxD"
                     /locus_tag="RHW33_000022"
     CDS             20555..20902
                     /gene="grxD"
                     /locus_tag="RHW33_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV1643579.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20953..25569)
                     /locus_tag="RHW33_000023"
     CDS             complement(20953..25569)
                     /locus_tag="RHW33_000023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV1643580.1"
                     /translation="MADNPNPSSPLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDQLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLQGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVASSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDVAHFE
                     STSGATSNRVQSSDIFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQHTVAAAAMDALQVDEWYSRVRRATPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNSGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEIASYLE
                     NLLTVKRIFPAMISGQERLACMDDAARLRDALGVQLPESLPAIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDPSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQAAQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFQALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25662..26309)
                     /gene="rnt"
                     /locus_tag="RHW33_000024"
     CDS             complement(25662..26309)
                     /gene="rnt"
                     /locus_tag="RHW33_000024"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV1643581.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26412..26819)
                     /gene="gloA"
                     /locus_tag="RHW33_000025"
     CDS             complement(26412..26819)
                     /gene="gloA"
                     /locus_tag="RHW33_000025"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV1643582.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26900..27997)
                     /gene="nemA"
                     /locus_tag="RHW33_000026"
     CDS             complement(26900..27997)
                     /gene="nemA"
                     /locus_tag="RHW33_000026"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV1643583.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28034..28633)
                     /gene="nemR"
                     /locus_tag="RHW33_000027"
     CDS             complement(28034..28633)
                     /gene="nemR"
                     /locus_tag="RHW33_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV1643584.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRDSHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            28736..28975
                     /locus_tag="RHW33_000028"
     CDS             28736..28975
                     /locus_tag="RHW33_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV1643585.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29024..29920
                     /locus_tag="RHW33_000029"
     CDS             29024..29920
                     /locus_tag="RHW33_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV1643586.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30001..30522
                     /gene="sodC"
                     /locus_tag="RHW33_000030"
     CDS             30001..30522
                     /gene="sodC"
                     /locus_tag="RHW33_000030"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV1643587.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30523..32535)
                     /locus_tag="RHW33_000031"
     CDS             complement(30523..32535)
                     /locus_tag="RHW33_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1643588.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32535..33392)
                     /locus_tag="RHW33_000032"
     CDS             complement(32535..33392)
                     /locus_tag="RHW33_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1643589.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSNSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33395..33631)
                     /locus_tag="RHW33_000033"
     CDS             complement(33395..33631)
                     /locus_tag="RHW33_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV1643590.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33832..34266
                     /gene="slyA"
                     /locus_tag="RHW33_000034"
     CDS             33832..34266
                     /gene="slyA"
                     /locus_tag="RHW33_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV1643591.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34312..34779)
                     /gene="slyB"
                     /locus_tag="RHW33_000035"
     CDS             complement(34312..34779)
                     /gene="slyB"
                     /locus_tag="RHW33_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV1643592.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35053..36162
                     /gene="anmK"
                     /locus_tag="RHW33_000036"
     CDS             35053..36162
                     /gene="anmK"
                     /locus_tag="RHW33_000036"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV1643593.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQVGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36260..36589
                     /gene="mliC"
                     /locus_tag="RHW33_000037"
     CDS             36260..36589
                     /gene="mliC"
                     /locus_tag="RHW33_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV1643594.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNSQR"
     gene            36648..37304
                     /gene="pdxH"
                     /locus_tag="RHW33_000038"
     CDS             36648..37304
                     /gene="pdxH"
                     /locus_tag="RHW33_000038"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV1643595.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37433..38707
                     /gene="tyrS"
                     /locus_tag="RHW33_000039"
     CDS             37433..38707
                     /gene="tyrS"
                     /locus_tag="RHW33_000039"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV1643596.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38769..39629
                     /gene="pdxY"
                     /locus_tag="RHW33_000040"
     CDS             38769..39629
                     /gene="pdxY"
                     /locus_tag="RHW33_000040"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDV1643597.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAK
                     PEHYFSATKL"
     gene            complement(39673..40278)
                     /gene="gstA"
                     /locus_tag="RHW33_000041"
     CDS             complement(39673..40278)
                     /gene="gstA"
                     /locus_tag="RHW33_000041"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV1643598.1"
                     /translation="MKLFYKPGACSLASHIILRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAESLK"
     gene            complement(40384..41886)
                     /gene="dtpA"
                     /locus_tag="RHW33_000042"
     CDS             complement(40384..41886)
                     /gene="dtpA"
                     /locus_tag="RHW33_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV1643599.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42497..43132)
                     /gene="nth"
                     /locus_tag="RHW33_000043"
     CDS             complement(42497..43132)
                     /gene="nth"
                     /locus_tag="RHW33_000043"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV1643600.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGIEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43132..43827)
                     /gene="rsxE"
                     /locus_tag="RHW33_000044"
     CDS             complement(43132..43827)
                     /gene="rsxE"
                     /locus_tag="RHW33_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV1643601.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43831..44451)
                     /gene="rsxG"
                     /locus_tag="RHW33_000045"
     CDS             complement(43831..44451)
                     /gene="rsxG"
                     /locus_tag="RHW33_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV1643602.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44455..45513)
                     /gene="rsxD"
                     /locus_tag="RHW33_000046"
     CDS             complement(44455..45513)
                     /gene="rsxD"
                     /locus_tag="RHW33_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV1643603.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45514..47736)
                     /gene="rsxC"
                     /locus_tag="RHW33_000047"
     CDS             complement(45514..47736)
                     /gene="rsxC"
                     /locus_tag="RHW33_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV1643604.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRRREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAAEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQANAVPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(47729..48307)
                     /gene="rsxB"
                     /locus_tag="RHW33_000048"
     CDS             complement(47729..48307)
                     /gene="rsxB"
                     /locus_tag="RHW33_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV1643605.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48307..48888)
                     /gene="rsxA"
                     /locus_tag="RHW33_000049"
     CDS             complement(48307..48888)
                     /gene="rsxA"
                     /locus_tag="RHW33_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV1643606.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48965..49405)
                     /locus_tag="RHW33_000050"
     CDS             complement(48965..49405)
                     /locus_tag="RHW33_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV1643607.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGAQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49491..49706)
                     /gene="ydgT"
                     /locus_tag="RHW33_000051"
     CDS             complement(49491..49706)
                     /gene="ydgT"
                     /locus_tag="RHW33_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV1643608.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49979..50104)
                     /gene="blr"
                     /locus_tag="RHW33_000052"
     CDS             complement(49979..50104)
                     /gene="blr"
                     /locus_tag="RHW33_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV1643609.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            50347..51387
                     /locus_tag="RHW33_000053"
     CDS             50347..51387
                     /locus_tag="RHW33_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1643610.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKDLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51423..52424)
                     /gene="add"
                     /locus_tag="RHW33_000054"
     CDS             complement(51423..52424)
                     /gene="add"
                     /locus_tag="RHW33_000054"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV1643611.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAQQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLNAEEKRALREKVAAK"
     gene            complement(52528..53700)
                     /gene="malY"
                     /locus_tag="RHW33_000055"
     CDS             complement(52528..53700)
                     /gene="malY"
                     /locus_tag="RHW33_000055"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV1643612.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53710..55302)
                     /gene="malX"
                     /locus_tag="RHW33_000056"
     CDS             complement(53710..55302)
                     /gene="malX"
                     /locus_tag="RHW33_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV1643613.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPLLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     STTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            55477..56505
                     /gene="malI"
                     /locus_tag="RHW33_000057"
     CDS             55477..56505
                     /gene="malI"
                     /locus_tag="RHW33_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV1643614.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56617..57384
                     /gene="hdhA"
                     /locus_tag="RHW33_000058"
     CDS             56617..57384
                     /gene="hdhA"
                     /locus_tag="RHW33_000058"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV1643615.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57613..58203
                     /gene="uidR"
                     /locus_tag="RHW33_000059"
     CDS             57613..58203
                     /gene="uidR"
                     /locus_tag="RHW33_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV1643616.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREVSFAQGLRAMIGGILTAS"
     gene            58593..60404
                     /gene="uidA"
                     /locus_tag="RHW33_000060"
     CDS             58593..60404
                     /gene="uidA"
                     /locus_tag="RHW33_000060"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV1643617.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQACNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60401..61774
                     /gene="uidB"
                     /locus_tag="RHW33_000061"
     CDS             60401..61774
                     /gene="uidB"
                     /locus_tag="RHW33_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV1643618.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61813..63078
                     /gene="uidC"
                     /locus_tag="RHW33_000062"
     CDS             61813..63078
                     /gene="uidC"
                     /locus_tag="RHW33_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV1643619.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(63123..64631)
                     /locus_tag="RHW33_000063"
     CDS             complement(63123..64631)
                     /locus_tag="RHW33_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV1643620.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEEMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64732..65907)
                     /gene="manA"
                     /locus_tag="RHW33_000064"
     CDS             complement(64732..65907)
                     /gene="manA"
                     /locus_tag="RHW33_000064"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV1643621.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            66106..67752
                     /gene="fumB"
                     /locus_tag="RHW33_000065"
     CDS             66106..67752
                     /gene="fumB"
                     /locus_tag="RHW33_000065"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1643622.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67895..69298
                     /gene="fumC"
                     /locus_tag="RHW33_000066"
     CDS             67895..69298
                     /gene="fumC"
                     /locus_tag="RHW33_000066"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV1643623.1"
                     /translation="MNTVRSEKDSMGAIDVPGDKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69295..70224)
                     /gene="tus"
                     /locus_tag="RHW33_000067"
     CDS             complement(69295..70224)
                     /gene="tus"
                     /locus_tag="RHW33_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV1643624.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLYDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70300..71601)
                     /gene="rstB"
                     /locus_tag="RHW33_000068"
     CDS             complement(70300..71601)
                     /gene="rstB"
                     /locus_tag="RHW33_000068"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV1643625.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQLTSA"
     gene            complement(71605..72324)
                     /gene="rstA"
                     /locus_tag="RHW33_000069"
     CDS             complement(71605..72324)
                     /gene="rstA"
                     /locus_tag="RHW33_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV1643626.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72454..72789
                     /locus_tag="RHW33_000070"
     CDS             72454..72789
                     /locus_tag="RHW33_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV1643627.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72786..73508)
                     /gene="folM"
                     /locus_tag="RHW33_000071"
     CDS             complement(72786..73508)
                     /gene="folM"
                     /locus_tag="RHW33_000071"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV1643628.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73545..74927)
                     /locus_tag="RHW33_000072"
     CDS             complement(73545..74927)
                     /locus_tag="RHW33_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1643629.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(75113..76057)
                     /locus_tag="RHW33_000073"
     CDS             complement(75113..76057)
                     /locus_tag="RHW33_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1643630.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76581..78113
                     /gene="pntA"
                     /locus_tag="RHW33_000074"
     CDS             76581..78113
                     /gene="pntA"
                     /locus_tag="RHW33_000074"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV1643631.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            78124..79512
                     /gene="pntB"
                     /locus_tag="RHW33_000075"
     CDS             78124..79512
                     /gene="pntB"
                     /locus_tag="RHW33_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV1643632.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79537..80571)
                     /gene="tqsA"
                     /locus_tag="RHW33_000076"
     CDS             complement(79537..80571)
                     /gene="tqsA"
                     /locus_tag="RHW33_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV1643633.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            80983..81348
                     /gene="mdtJ"
                     /locus_tag="RHW33_000077"
     CDS             80983..81348
                     /gene="mdtJ"
                     /locus_tag="RHW33_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV1643634.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81335..81664
                     /gene="mdtI"
                     /locus_tag="RHW33_000078"
     CDS             81335..81664
                     /gene="mdtI"
                     /locus_tag="RHW33_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV1643635.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81703..82524)
                     /locus_tag="RHW33_000079"
     CDS             complement(81703..82524)
                     /locus_tag="RHW33_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV1643636.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82624..82707)
                     /locus_tag="RHW33_000080"
     CDS             complement(82624..82707)
                     /locus_tag="RHW33_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643637.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82800..83135)
                     /locus_tag="RHW33_000081"
     CDS             complement(82800..83135)
                     /locus_tag="RHW33_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV1643638.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            complement(83532..84785)
                     /locus_tag="RHW33_000082"
     CDS             complement(83532..84785)
                     /locus_tag="RHW33_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1643639.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKSLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            84892..85785
                     /locus_tag="RHW33_000083"
     CDS             84892..85785
                     /locus_tag="RHW33_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1643640.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            85920..87140
                     /gene="mlc"
                     /locus_tag="RHW33_000084"
                     /gene_synonym="dgsA"
     CDS             85920..87140
                     /gene="mlc"
                     /locus_tag="RHW33_000084"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV1643641.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            87265..87960
                     /gene="bioD"
                     /locus_tag="RHW33_000085"
     CDS             87265..87960
                     /gene="bioD"
                     /locus_tag="RHW33_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV1643642.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(87913..89205)
                     /gene="clcB"
                     /locus_tag="RHW33_000086"
     CDS             complement(87913..89205)
                     /gene="clcB"
                     /locus_tag="RHW33_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV1643643.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSTWRRLLTPALGGLAAGLLLMVWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLSGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(89364..89978)
                     /gene="dmsD"
                     /locus_tag="RHW33_000087"
     CDS             complement(89364..89978)
                     /gene="dmsD"
                     /locus_tag="RHW33_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV1643644.1"
                     /translation="MTHFSQQDNFSIAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(90021..90875)
                     /locus_tag="RHW33_000088"
     CDS             complement(90021..90875)
                     /locus_tag="RHW33_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV1643645.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKTDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLSSIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(90877..91494)
                     /gene="dmsB"
                     /locus_tag="RHW33_000089"
     CDS             complement(90877..91494)
                     /gene="dmsB"
                     /locus_tag="RHW33_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV1643646.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYTG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(91505..93928)
                     /locus_tag="RHW33_000090"
     CDS             complement(91505..93928)
                     /locus_tag="RHW33_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV1643647.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERTPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(93989..96415)
                     /locus_tag="RHW33_000091"
     CDS             complement(93989..96415)
                     /locus_tag="RHW33_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV1643648.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVVWGACSVNCGSRCALRLHVKDNEVIWVETDNTGNDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKNVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            complement(96614..96919)
                     /locus_tag="RHW33_000092"
     CDS             complement(96614..96919)
                     /locus_tag="RHW33_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV1643649.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            97027..97737
                     /locus_tag="RHW33_000093"
     CDS             97027..97737
                     /locus_tag="RHW33_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV1643650.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(97740..98300)
                     /gene="speG"
                     /locus_tag="RHW33_000094"
     CDS             complement(97740..98300)
                     /gene="speG"
                     /locus_tag="RHW33_000094"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV1643651.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(98335..98676)
                     /locus_tag="RHW33_000095"
     CDS             complement(98335..98676)
                     /locus_tag="RHW33_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV1643652.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            98811..99137
                     /locus_tag="RHW33_000096"
     CDS             98811..99137
                     /locus_tag="RHW33_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV1643653.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASVWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            99343..100557
                     /gene="rspA"
                     /locus_tag="RHW33_000097"
     CDS             99343..100557
                     /gene="rspA"
                     /locus_tag="RHW33_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV1643654.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            100569..101588
                     /locus_tag="RHW33_000098"
     CDS             100569..101588
                     /locus_tag="RHW33_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV1643655.1"
                     /translation="MKSILIEKPNQLAIVKREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHVDGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWVINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQLITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIEPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            101646..103013
                     /gene="ydfJ"
                     /locus_tag="RHW33_000099"
     CDS             101646..103013
                     /gene="ydfJ"
                     /locus_tag="RHW33_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HDV1643656.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQ
                     PSRSL"
     gene            103103..104563
                     /locus_tag="RHW33_000100"
     CDS             103103..104563
                     /locus_tag="RHW33_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV1643657.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEYFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GGDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(104599..104802)
                     /gene="ydfZ"
                     /locus_tag="RHW33_000101"
     CDS             complement(104599..104802)
                     /gene="ydfZ"
                     /locus_tag="RHW33_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV1643658.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(104979..105665)
                     /gene="ydfH"
                     /locus_tag="RHW33_000102"
     CDS             complement(104979..105665)
                     /gene="ydfH"
                     /locus_tag="RHW33_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV1643659.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHLLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(105754..106500)
                     /gene="ydfG"
                     /locus_tag="RHW33_000103"
     CDS             complement(105754..106500)
                     /gene="ydfG"
                     /locus_tag="RHW33_000103"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV1643660.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDSKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            106637..108682
                     /gene="dcp"
                     /locus_tag="RHW33_000104"
     CDS             106637..108682
                     /gene="dcp"
                     /locus_tag="RHW33_000104"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV1643661.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAHADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRFATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(108727..109245)
                     /locus_tag="RHW33_000105"
     CDS             complement(108727..109245)
                     /locus_tag="RHW33_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV1643662.1"
                     /translation="MNINRNKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            109521..109913
                     /gene="ydeI"
                     /locus_tag="RHW33_000106"
     CDS             109521..109913
                     /gene="ydeI"
                     /locus_tag="RHW33_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV1643663.1"
                     /translation="MKFQPIVLASFLVMPYALADDQGGLKQDAAPPPSHAIEDGYRGT
                     DDAKKMTVDFAKTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            110168..111058
                     /gene="dgcZ"
                     /locus_tag="RHW33_000107"
     CDS             110168..111058
                     /gene="dgcZ"
                     /locus_tag="RHW33_000107"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV1643664.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQGAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(111277..111372)
                     /gene="mgtS"
                     /locus_tag="RHW33_000108"
     CDS             complement(111277..111372)
                     /gene="mgtS"
                     /locus_tag="RHW33_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV1643665.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(111499..112686)
                     /gene="ydeE"
                     /locus_tag="RHW33_000109"
     CDS             complement(111499..112686)
                     /gene="ydeE"
                     /locus_tag="RHW33_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV1643666.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVDSVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTVGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMFFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGLVLTNLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            112881..113780
                     /gene="eamA"
                     /locus_tag="RHW33_000110"
     CDS             112881..113780
                     /gene="eamA"
                     /locus_tag="RHW33_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV1643667.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSFNGQHVAMLGFILTLAAAFSWASGNI
                     FNKKIMSHSSRPAVMSLVVWSALIPIIPFFVASLILDGSGKMIHSLVTIDMTTILALM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMAGLYINVFGLQWRKAVKVRG"
     gene            complement(113811..114029)
                     /gene="marB"
                     /locus_tag="RHW33_000111"
     CDS             complement(113811..114029)
                     /gene="marB"
                     /locus_tag="RHW33_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV1643668.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTQQPLVTSCGDVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQQAM"
     gene            complement(114061..114444)
                     /gene="marA"
                     /locus_tag="RHW33_000112"
     CDS             complement(114061..114444)
                     /gene="marA"
                     /locus_tag="RHW33_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV1643669.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(114464..114898)
                     /gene="marR"
                     /locus_tag="RHW33_000113"
     CDS             complement(114464..114898)
                     /gene="marR"
                     /locus_tag="RHW33_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV1643670.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            115110..115775
                     /gene="marC"
                     /locus_tag="RHW33_000114"
     CDS             115110..115775
                     /gene="marC"
                     /locus_tag="RHW33_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV1643671.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(115800..116990)
                     /gene="ydeA"
                     /locus_tag="RHW33_000115"
     CDS             complement(115800..116990)
                     /gene="ydeA"
                     /locus_tag="RHW33_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV1643672.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(117141..117847)
                     /gene="yneK"
                     /locus_tag="RHW33_000116"
                     /pseudo
     CDS             complement(117141..117847)
                     /gene="yneK"
                     /locus_tag="RHW33_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT        29721 a        28678 c        30421 g        29027 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctac tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagtcgt cgatgcccat atggggggct tttttgcggc gcaaatgaaa
     1141 ttcgccggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggt
     1381 ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta tcggtgcgaa taacaaccat gtcgtgccaa gtacgccaca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggcggtc caattgaaac gggtgaaatt ccgccaggca atcagaatac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agcggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtcaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc
     1861 atcttcccga acgggccgag agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatttgtt cgatgactac ggcctgtggt gtaactacgg gcagctgcat
     1981 cgcgacttta cttattgtta cagcaaaggt gtgttcaagc gtgttctgcc agctgaagag
     2041 tatgcggaaa ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaagatttt
     2101 tactaccgtc tggcgcatcg tgtgggtgag ctgagccacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg
     2221 tcgccgtttg gctatccggt tcaccatgcc aatgaagcgt cagcgcaggt cggttccatt
     2281 gttaactgta tgtttaaccg tgactgcatg acgcataccc atatcaactt tattggttcc
     2341 ggcttgccat tgaaactgca acgtgaagtg gcgaaagaac tttttggttc tgaagatgct
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcactctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattac cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcactggcga agagatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacat tgcatcgtgc ctacacggtg aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagatccc ggtctttact
     2761 gaaggtactg acaaaatgga tcgtgatgat atgcatgcct cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatgggt
     2941 gagatgaacc atcgggatga attgccgtta gcgaaagtca gcgaagttga cgaggcgaag
     3001 cggcaatggt tacaggggat gcgtcacccc gttgatacgg taacggaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggaac tgtcagtttt gctggtaaac
     3181 atcaagcaga acggagacta cgcggatatc gcgtgtatga cgggaagcca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcggcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccct tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacaaag tcgccatgct gatgcaagcg ccatattact tccaggaagc gcagattgaa
     3421 gccgcgatcg ccgcgatgga tgttgcacct gaatatgccg atatacggca ggtggagtca
     3481 tcgacggcgg tgttgtactt gtttagcgag cgatttatga cctatggcaa ggcttatggc
     3541 ttatgcgaat ggttcgaggt agagcagttt cagaacccct gaaatttcag ggagatgtca
     3601 tggatagggt ggacagttgc agccactgca ttaggggata atcagatgtc cttcactcga
     3661 cgcaaatttg ttctggggat gggaacagta atctttttta ctggttctgc gtcttctctg
     3721 ttagcgaaca cgaggcaaga aaaggaagtt cggtatgcca tgattcatga cgagtcacgg
     3781 tgcaatggct gtaatatttg cgcccgcgca tgccgtaaaa ccaatcacgt ccccgctcag
     3841 ggaagccgtt tatcgatagc acacattcca gtcaccgata acgacaacga aacgcagtat
     3901 cacttttttc gccagtcatg tcagcactgc gaagatgctc cgtgcatcga cgtatgcccg
     3961 acaggggcgt cgtggcgtga tgagcagggg atcgtgcggg tagaaaaatc gcagtgcatc
     4021 ggctgcagtt attgcattgg cgcttgtccg taccaggttc gctaccttaa ccctgtcact
     4081 aaagtggcag ataaatgtga tttctgcgct gagtcccgac tggcaaaagg ctttccgccc
     4141 atttgcgtca gcgcctgccc ggaacacgca ttgatctttg gtcgcgaaga tagcccagaa
     4201 attcaggcgt ggttgcagga taataaatac tatcaatatc agctacctgg tgccgggaaa
     4261 ccgcatctgt atcgccggtt cggtcaacat ttgattaaaa aggaaaatgt atgaacccgt
     4321 cgcaacatgc tgaacagttt cagagccagt tagcgaacta tgtgccacag ttcactcccg
     4381 aattttggcc ggtgtggttg atcattgccg gagtattgct ggttgggatg tggttggtgc
     4441 tggggctgca cgccttgctt cgtgctcgtg gcgtgaagaa atcagccacc gatcatggtg
     4501 agaagattta tctttacagc aaagcggtca gattatggca ctggtcgaat gcgttactct
     4561 ttgtattgtt gctggcaagt gggctgataa atcactttgc gatgctgggc gcaactgcgg
     4621 ttaaaagtct ggttgcggtg catgaagttt gcggattttt gttactggca tgctggcttg
     4681 gctttgtgct gatcaatgcc gttgggggta atggccacca ctatcgcatt cgtcgtcagg
     4741 gttggctgga gcgggcggca aaacaaacgc gattttattt gtttggcatt atgtaggggg
     4801 aagaacatcc tttcccggca acaacccagt ctaaatttaa tcccttacag caggtcgcct
     4861 atgttggtgt catgtatgga ttgctgccgt tgttactatt gacggggctg ctgtgtctct
     4921 atccgcaagc cgtgggagat gtgtttcctg gcgttagata ctggttattg caggcgcatt
     4981 ttgctctggc atttataagc ctctttttta tcttcggcca tctttatctt tgcaccacgg
     5041 ggcgtacgcc acacgaaacc tttaaaagca tggtcgatgg ctatcaccgg cactaacgaa
     5101 tgatcattac acgagcagac ctgcgagaat ggcgtatcgg agcggtgatg tatcgctggt
     5161 tcctgcgcca tttcccacgc ggcggtagtt atgccgatat tcatcatgca ctgattgagg
     5221 aaggatatac ggactgggcg gagagtcttg ttgagtatgc gtggaaaaaa tggctggctg
     5281 atgaaaactt cgcgcatcag gaagtttcct ctatgcaaaa actggccaca gatcccggag
     5341 agataccatt ttgcagtcag tttgcccgca gcgacgatca tgctcgcatc ggctgttgtg
     5401 aggataacgc cagaatcgca accgctgggt atgccgtgca aattgcgagt atgggttata
     5461 gcgtgcggat tggaagtgtc ggctttaaca gtcacattgg cagctccggt gaacgcgcac
     5521 gggttgctgt aaccggcaat tcttcgcgga taagcagcgc tggagatagc agtcggattg
     5581 cgaataccgg aatgcgagta cgcgtttgta cattgggtga acgatgtcat gtcgccagta
     5641 atggtgacct ggtgcaaatc gccagctttg gcgcaaacgc cagaattgcc aacagcggcg
     5701 acaatgtaca catcattgcc agcggtgaaa attcaacggt agtgagcacg ggcgtggtcg
     5761 actcaattat tctcgggcca ggaggaagcg ccgcactggc atatcatgac ggcgaaagag
     5821 ttcgtttcgc cgtcgccatt gaaggtgaaa acaatattcg agccggagtc cgctatcgtc
     5881 ttaatgaaca gcatcagttt gttgagtgct gatagcttta cctttaatca aacgaaagac
     5941 gccgacgagc acgggatgca ggttttacga ccgcccgagc catcgcctca ccaatttctg
     6001 cacatgccgt aagtccctga acgaaaggct ggtcgtgcat taaccagggc aacgcgccga
     6061 acgctacgcg cccacgaata tcttcgggtt gctgtaacgt ataatcttca ccaacatcag
     6121 ggatttcatc ccctgttttc tgtaattgtt cgcgtagtcc agggaaagga atatctttca
     6181 ctttaagcgg acgctgcccg cgggcatcaa taaaaacgtc aaacgagtag ctgttgtctt
     6241 ccgttttcag gacggtgcgc gactcgttaa tttccatttt gtagtcttca ccgagggcga
     6301 gaatatgaat gattcccgct tcacgtaagg caagtaggcg acgaatagac tctgaaggga
     6361 ttgcggcata attatcgatg aaaacccggg caaggccttt actgaaccgt ttatggtcgt
     6421 gttcattcag atgtggaaca atttcctgta cggcttcatg caggcgcaga atgacataac
     6481 gccagggaac agtatgtttt tctcgtttat tgcgttcgac ttcctggaga tttttttctg
     6541 cccagtcaaa tgggtcgcgt tgtttgcgct cggcaaacca ggcttgagca aaggaatcga
     6601 cattcaggct ctctaaggct atgcgttgac tccagtctgg atcagcaaac ttgatttcct
     6661 ctactatcaa tctaaatacc cgatccaaaa ggccctcttc gcctttttga atctcagcat
     6721 ttaatgcctg atcggtgacg atgtgtaagg gctcgtaggg aatagggcaa tagaaatcgg
     6781 cctcgggtaa aatacccgtg cgcgacatca acgtgatatt tagcttttca cttgcgttat
     6841 cgcggtgaaa aacgacgtgt tgtttatcat cttcaatgaa cgaaccatgc tgaatagcca
     6901 ctgccattgc cgcatccagt ccgctcaagg atgttcccat aatacccacg ttacacgcat
     6961 cgacctttgc ttccatcagg cctgaccacg ggctgggaaa atacgttcgg gttgcttctt
     7021 cttcatcagg ccagacgtga cccgtggcaa tcaccgctaa atcaaacgtc tcgctgggta
     7081 aatcctgatt tgtagcgagc atgacgccag catttgtaat ttgcagatcg gtaacttggc
     7141 atgattcata aacagccact gcaaattttt gctttcgtgc ctggtctact aatcgtaaaa
     7201 attgatcgcg gaaatattcg cccagcagaa ttcgcggtaa aaactgacga tcgtgcaagg
     7261 tttctttttt aacgccataa cgctggagat ggctggcttc ttgcttttgt agccattcga
     7321 gatacgtaca ataaatcggc ggtatttcaa tactggcaat atttgccagc atcatttttg
     7381 agttttcctc atcactgtat ggcatcccga caccggcctc gtcagcctgc tcgaagatag
     7441 aaatagaaag tggagtttgt tgctgtagaa gcgagaataa ggtgtagatc cccgtaggcc
     7501 cggcacccac aatagcaatt tttttcattg ttacttacct gtacgcattc tttcgaattt
     7561 agtgttatca gataagtttg gttgagattt ttagaatttg agaaatttca gacagggatg
     7621 aaaagggcgc taaccgccct tgattaaatt ctgtcaggcg agttttgcct gattgatttg
     7681 actcagtggc tcattgacat cgaaaacttt ggcgacaact tcctcaacgc cgagattttt
     7741 cagtcgggca gtgtgttttt ccagataggc aagggcgctt ttttcatcag tgaaaaggta
     7801 gatcccaccg gcttcgtggt tcttttcact ttcggtccat accttccaca gaaaaccagg
     7861 ttcctgatta atcgactcag caagcggttt aagctgctca gccattgcgt cgccaaatgg
     7921 gccattgaaa gaaaaatgaa gttgtaacaa agttgccata gcgattctcc ttctgataac
     7981 gcatacgaag acagtgtaaa tcttttaacg tattggatac agagaagaag cagagggaaa
     8041 aaggagaaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatt cgtgtatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc ctccgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaat gtgcaaattg
     8581 ttaagaccgg cgaggtcatt ggcgcgacgc aacttactga aggcgagttg attgttgaag
     8641 ctggcggaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtgacaaccg
     8701 gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga taaaggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg ccatcgcgcg gaaaaaatta
     8941 ttctcgatca agagggcggc ctgttggtca atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga gttgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcg gggaaagcca atgatacgtt gcttgctggt ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggagccatc tatcgtctgg
     9181 ggacggatgg tcttcagctc tacagttccg gtaagacgca aaacctgtcc gttaatgtgg
     9241 gtggccgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtggaacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccgggagt gttgcattac tggacggcgc ttcaatgatt attggctatg
     9421 gcgcagatct gcaacaatca acgattactg tacagcaggg cggtgtattg attctcgacg
     9481 gcagtacggt aaaaggtgac agtgtcactt tcagtattgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact ggtgcggcaa cgcatgtgca gctgaaagtg aaacgcctgc
     9601 gcggagaggg agcgatttgc ctgcaaatca gtgcgaaaga aatttcacct gacttcatca
     9661 atgtgaaagg ggaagttacc ggggatatac acgttgagat aacagatgcc agtcggcaaa
     9721 ctctgtgtaa cgcactgaaa ctacagccag acgaagacgg gattggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc aacccgcgaa ggttgcgcct tgtctttatt agcgggatgc
     9841 tcgttgcaga atgatggctg acggcagacg ttgcaggaac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca gtgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtgatataga aaatggaacg cgtatcttta taaccacgca aaatcccact
    10081 gccaatcacc tggattgagt cagaaatctg atataccgcc gccagcaaca tcaaatgcgc
    10141 agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcagccagcg ttgagccctg acccagacga taacctacgc ggatagttac
    10321 cgctgccgcc agcgacattg gaagcacgaa cattagtgaa ctaaagttca gggcaatctg
    10381 gtgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 tgtcacttca aagaacagcg ccagcgcaat cggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggtttcg cggtgccttt ttcgttacga atatcgcgca tcgagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca acgccaccaa gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 caccggaatg ttcaccagca ggccgataaa gcccattacc atacccggct tggtttttgc
    10741 cagaccttca cattggttac gggcaacctg gaagaaaaga tagcccggcg cgccccacag
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcggataatg taacctgcat tccacagcac cagcataatg agaacggaaa caaaacccgc
    10921 cagccagaag ccctgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccgcctg aatgggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagt cattcttacc
    11341 ccttcactgg taagggcgtt ttccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gcacacatgt ggtggagtta cccgaccaca tgctggacgg
    11521 cctggaaacc ggtgcgtccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacca atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagtg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac caccgctgaa gtggcgaaaa tattaacctc
    11761 ggaaaataat cgccagatct ggtttaaagt ccaggatagt cagttgatga aatatattct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac actggaacgc acgactcttg ggaagaaaaa
    11941 acttggcgca cgcgtcaaca ttgaaatcga tccacaaact caggcagtgg tagatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac accacctgcc agagctgaat atcacgggcg cggaaagcac
    12181 ctgcacaggc attcagataa taggtaaaca ttcgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaacgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca caaagtgggg ttcgctggat tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt gttaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatagtat ggagcaggaa tataccttcc ggtttcaaat
    12481 tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgc tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgccaaccac gcttacgtca taattagacg ccatgtagtg tgccagtccg ccccagccgc
    12721 agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagcg caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgagcgccg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg gggagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagagccttc ttgcagaacg cgtttaaaaa aatcggggtt tttcacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttgtcatc cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc
    13381 cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaaaaac ataatattta
    13441 tcgtagcttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ctatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 cgcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgatcagc caggaaacta ccagacttgc gaggaagcac agacccagtt gaagagtgtt
    13741 ctgcaacgct gcggcgcgac cagttgcgtg tgggaaggga cgcagcgcct gggcgaccac
    13801 aatcgggtag atcgcgccgt tggcaatcgc catcacacag aatgggatca ggatttcgac
    13861 cagcgacaca tggctaatga agcccgcagc ccaggtcgca atgacactga cagcaaacag
    13921 caccagcaac cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtagccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgagcaggcc gcgtatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa aagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagagg agccagggcc ggagatagac ccaccagcgg
    14341 catgatggtc gcaaaaattc ggttaacttt ctgtgaagga taataatccg tcactaacgc
    14401 ttgccagata accgccgcgg cgcagacacc cacagcctgt acaaaacgca ataccagcag
    14461 cgtagcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gattaataat accggtttac gaccataacg gtcggagagc ggcccccaca gaagctgggc
    14581 tgctgcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 tacgctcaaa cccgccagcc agactaaaaa tcttttccca ggtagcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gactggtttc aaaaattttg caggcagact atgtggtcag aatattcact
    14881 cgaagttgtt gatgcggtag cgcgtaatgg tagttttagc gctgcggcac aggagttgca
    14941 tcgcgttcct tctgcggtca gctataccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctctttgaa agacggcacc gtgatgtgga actgaccgct gccggagcgt ggtttctcaa
    15061 agaagggcgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggccagt tagctatcgc ggtggataat attgtcaggc cagaacgtac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcgct ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgctaag
    15361 ctggagttgc gttgttgcca gtcaccaccc gctggcgttg atggatggcc cgttcagcga
    15421 tgatacgttg cgcaactggc cgtcgttggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 acgtattacc tggttgctgg ataaccaaaa aagacttgtc gtacctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaagggaagt gggtagcatt agaactggaa aacccctttc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggt tgcttgagta
    15721 tctcggcgat agtgagactc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caagcgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa
    15901 cagcatgttg aacgctgttt cacccagcga atcttttggc tgatggatcg tggtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 gacgcgcagc cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcggctg cgacaggatt tgctgcatgg cgcgataacc
    16141 ggattcaggt tcaaagtcac cctgcacaat ccagctttcc ggcaccttga tcattgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgcca gtgttacgtt ccagcgggcc
    16261 ggggataacg ccgatttcgc ggtgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaac gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt acccagaatc agggtgtagc ctttctggaa
    16561 gcaatttttt tcaactgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttgatcg ctgcccacac ggcgttgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaaatcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaag tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaatagtgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagtg gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattgctgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggc
    17401 gattggcgcg tttggtatcg ggacaacgga gttctcgcca atgggcttgt tgcccgtcat
    17461 tgcgcgcggt gtggatgtct cgattcccgc tgccggaatg ttaatcagtg cctatgcagt
    17521 tggcgtaatg gttggcgcgc cgctgatgac gcttctactt tctcatcgtg cccgccgcag
    17581 tgcgttgatt ttcctgatgg caattttcac gctcggcaac gtactttccg ccatcgcgcc
    17641 ggattatatg accctgatgc tttcacgcat tttgaccagc ctgaatcacg gagcattttt
    17701 tggtttgggt tcagtcgtgg ccgcaagcgt ggtgccaaaa cataaacagg ccagcgcagt
    17761 tgccactatg tttatggggt taaccctggc aaatatcggt ggcgtgccgg cggcgacctg
    17821 gttgggtgaa accatcggct ggcggatgtc atttctggca acggcggggc tgggcgtgat
    17881 ttcaatggta agtctgttct tctcattacc taaaggtggt gcaggggcac gacctgaagt
    17941 gaaaaaagag ctggcggtat taatgcgtcc gcaggtgctg tctgcattgc tgacgacggt
    18001 actgggagct ggtgcaatgt ttactctcta cacctatatc tctccggtac tgcaaagtat
    18061 tacccacgca acaccggtgt tcgttacggc aatgctggtg ctgattggtg tcggattctc
    18121 tatcggtaac tatctcggcg gcaaactggc agatcgttca gttaacggca cgttgaaagg
    18181 ctttttgttg ctgctgatgg tgattatgct ggcaatcccg ttcctggccc gcaatgagtt
    18241 cggcgcagct attagcatgg tggtgtgggg cgcagcaacc tttgcggtcg taccgccgtt
    18301 acagatgcgc gtgatgcgtg tcgccagtga agcgccaggt ctgtcttcat cagtcaatat
    18361 tggtgccttt aatcttggaa atgcgctggg agcagctgct ggtggtgcgg taatttccgc
    18421 tgggctggga tacagctttg tgccggtgat gggggcgatt gtcgcgggac tggcattatt
    18481 gctggtgttt atgtcagcca gaaaacaacc tgaaacagtt tgcgttgcta acagctaatt
    18541 aaacaaaaac gcagaaggat aaccttctgc gttacagttg ctgacaacgt gcgatttatc
    18601 ttgccgaatg cgctgttaac gcctcatccg cgttggcatg gtaagcgaag cgcatcaggc
    18661 aatactgcat ttgccatcag ttattatgca gcgagatttt ttgctacgaa ttcccagttc
    18721 accagcgccc agaagtgctc aagatagcca ggacgtgcat tgcgatagtc gatgtaatat
    18781 gcgtgttccc agacatcaac ggttagcagc ggagtcgcat cggtggtcag cggagtaccc
    18841 gcgttagagg ttgaaacgat agccagtttg ccatcgctgt ttttcaccag ccaggtccag
    18901 ccagaaccaa agtttttgat cgctgcatca gtaaactgag ctttgaaatc ggcaaagctg
    18961 ccaaaagatg cggcgatagc ttcagcgact tttccagtcg gttcgccacc ggcgttcggt
    19021 gccaggcagt tccagtagaa agtatggttc cagacctgag ctgcgttgtt gaatacgcca
    19081 ccttcagagc tgcgaataat ctcttccagt gatttacctt caaacgcggt acctttgatc
    19141 aggttgttca ggttagtgac ataagtctga tgatgcttgc cgtagtgata ctcgatggtt
    19201 tccgcagaaa tatgcggtgc cagagcatct ttagcatatg gtagtgcagg taattcgaat
    19261 gacattgcta ctctccttta ttattaattt gcatacgtac aatagcctta ttgtgcgtat
    19321 ggatagggta gcaaattaaa aggtgagaca aaagatgaac ttaccctgtt gccgtaacaa
    19381 cagggtaaag ttttagcgaa gtgtttttgg ggtcattacc cggcgagcgc caacgtagtg
    19441 gcgctgccag tagtcttcac tgagagaagt gatttgaatt tcctgacccg tacgcggtga
    19501 ctgaataaat ttgccgttgc cgacatacac accgacatga tcggctgtac cgcgtccctg
    19561 agtacggaaa aagaccaggt cgccgttttt cagctcacta cgttcgatag gtgctgcatc
    19621 acgcaggtga tacatttcat tcgccgtacg cggaatacga attttcacca aatctttata
    19681 agcgtaataa accaggccgc tgcaatcaaa accggtacgc ggtgagctgc caccccaacg
    19741 atatggctta ccaatttgct gcatcagttt attcattgcc actttcgtag ctttttgcac
    19801 cttcgctttg tgcgcgtcgg caagagttcc tgacgcggca tttttgactt tcacacaatg
    19861 cgatttatag cccttacgtt tggtacactt ttcggtaaag ctggctgagg ccgttttatt
    19921 gacggcgtgt ttgctgcgag ttttggcgtt tttagaagcg gtaatggaag attttttaac
    19981 ggaagaagcg gttttgcttt tggtcgtact tttactggcg gcttttttcg ctgttttctg
    20041 gctggctttc tgggtctttt tggtggttgc cgtgcttttc tttttatctg cttttttggt
    20101 gatatgagtg gtagcagaac tctccctggc ttgctttgaa gcatgggcca taggcgttaa
    20161 aggcagggtg gtaaaaagta aagcacagag cgtgatcgaa atacggttta tccgcgccac
    20221 tgagcgtccc tctgttaagg caggtctatc atgcctgcgt ctgatttaca aaacttgatg
    20281 attctaatgc atagaagaag agaaagttaa tagcttttat tgttaaaaaa tacgtttcgt
    20341 tggcgagcgc aaaaaaatgc agcagtgaaa gctaaaaatg gtcataaata cgccagtttg
    20401 ccgtgcatca gggaagaaac gaatatttga aatgcaacaa atagagcaaa gggataaaat
    20461 agcaaaagcg tgatgaaaat gttattttcc gatgccgcac tgacccgcta caatgtcaga
    20521 ttactgccgt aaagaagttt gaggaagcaa gacaatgagc accactatcg aaaaaatcca
    20581 acgccagatt gctgaaaacc cgatcctgct gtacatgaaa ggttcaccga aactgccgag
    20641 ctgcggtttc tctgcccagg cagtccaggc gcttgccgca tgtggcgaac gttttgccta
    20701 tgttgatatt ctgcagaatc cggacattcg tgcggagctg ccgaaatatg ctaactggcc
    20761 gaccttcccg caactgtggg ttgacggcga actggtcggc ggttgtgata tcgtgatcga
    20821 aatgtatcag cgtggcgaac tgcagcagct gatcaaagaa actgccgcta aatacaagtc
    20881 tgaagagccg gacgcggaat aatccgtcct gcaatactga cggatgcgaa cgggcatccg
    20941 tcagtccttt ctctatcccc aatccagccc ttgtggcgaa cttgaaaatc ccgcttcacg
    21001 cagcagcgta aacatcggcg tttgccggac cggtagatca ttcacttctg ttagcgtaaa
    21061 gcgcagccgt ggttcgcgac gcagtgcggt agtcagcgct tggaaaacct ctggggcgag
    21121 taattcctct ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa
    21181 caatttgccg ccagaaacga ccaccagcgc acccgcccga cgcgttggta ccagcgttgc
    21241 cggatgtgca ggccagggca gaagatttcc ccacacatta gccggatcgt tggcggagag
    21301 cgccactggt gtatagtggc gcgtttgcgc ggcttgtgtc gccaggtcgc gcaagcggtc
    21361 aatagtcaga cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat
    21421 aattcgcccg gaatcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg
    21481 gatattttcg gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc
    21541 cgccagcgcc agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg
    21601 tccagccaga tttggtgtgt tgtaagatac ccgcggcgag acgggttgtg catagacagg
    21661 acgcccacga cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg
    21721 ggtgagggcg cgtaacggtg cccaaatatc gctggtgatg acgccttgcc agaccagcgc
    21781 ccataacgcc tcttgcagtg ccgagggatc aaccgattcg ccgattttgt cgcgtatccg
    21841 ttggctgatt tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg
    21901 ttgcagcgcc gaacgattcg cctgatccgc ttcggcggga gtgaacgatt ctgcagcata
    21961 ttcctgtaga tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca
    22021 gataaccgca ccggttgcca gtaattcatc gagcatttct ggcgaatagt cgcgtacgcg
    22081 ggcaggcagg atctggcttt cccagagtga ggcgggtaag ccgactccgg caagctgttc
    22141 gattacccgc atcacgccat ccacgccctc ataaacgcct ggcgatgttg aggcaaagag
    22201 cgccgggcta ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata
    22261 ggtcgtggct gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag
    22321 acgacgaaat acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg
    22381 ttcgcgcagt tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc
    22441 cagttgttca gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag
    22501 cgggtaactg actctatgca aataaatcgc tggcagagac tctggtagtt gtacgccgag
    22561 ggcatcgcgc agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat
    22621 cgccgggaag attcgtttta ctgtaagaag attttccaga tacgacgcaa tctcttcact
    22681 gctgcctgta tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag
    22741 gtcgaacaat ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc
    22801 ttccacctgg cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag
    22861 attgcgcagt aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc
    22921 atcgctctgg tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga
    22981 gggcgtagta gtcgttacat cggatatttg aatttcgcca ctgttcaggc gacgcatcaa
    23041 acgttccagt gcgggaagat cataaacatc ttgcagacat tcgcgtaggg tttcgagaat
    23101 gaccggaaaa tccggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg
    23161 ttgccagagc ggggtgcgat ggcccggcgt gcggcccggc attaataacg cccgcgcggc
    23221 gcattcgcga aaacgggcgg cgaaaagtgc tgagctgcct accgcctcgc ggacaatttg
    23281 cagcaacttt tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc
    23341 aggaatgcgc gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgccccatag
    23401 cgcatgtatt cgcccggcaa tcgccagcgc ccagggttca tgcacccgtc ttccataggg
    23461 agagtgcaaa ataatacgcc agtcgccaat ttcatcacgg caccgttcga ggaccagatg
    23521 acgactcccc ggaacgatgc ccgtcgcgtt gcgctgctcg tcaatcaacc cctgaatatt
    23581 ggcgatcgta ttttcttcag ccagccacgg gggaatagtc ccggaaaaga acgcgccatc
    23641 cgccagtaaa tgaagaaaat cgccgatcat ctcgcctaat tcagccggac gtccgttacc
    23701 ttcaccacgc cagaagggga gccgggcaga acgacccgga gcaggagtca caatcacctg
    23761 atcgcgggtg atttgctgta tccgccatga ggtagcgccg agagtgataa tgtcgttcac
    23821 ccgcgactca tataccatct cctcatccag ttcacccacc cgccgcgaac cggcctgttc
    23881 ttcaccttcg ggtaataaca cgctatacat gccacgatcc ggaatggtgc caccgctggt
    23941 aaccgccaac agctgagcgc caggtcgggc ggtcaatatc ccggtctccc tgttccagac
    24001 cagttttggt cgaaaagcag aaaaatcgcc agagggatag cgcccggaaa gcatatccag
    24061 cgtggcgtca aaaacacgtc ttggcagatc tttccacggt gtggcacggc gtacgcggga
    24121 gtaccattcg tctacctgta atgcatccat cgccgcggcg gcaacggtgt gctgcgcaag
    24181 gacgtcgagt ggattatgcg gtggtgtcag gttttccagc ctgcctgcga acatacactc
    24241 tacaatgact gcggaatcga ctaagtcacg ccgggttcgg gggaaaaaca gccctttaga
    24301 tacaccgcca acctgatgtc cggcgcgacc aatgcgttgt aacccactgg caacagaaag
    24361 cggcgttgcc acttgaatca ccagatccac agcgcccata tcaatcccca gttcgagact
    24421 ggaggttgcg accacgcagc gtaattcacc cgatttcagc gcctgttcgg tgattgctcg
    24481 ttgttcttta gagacggagc cgtggtgtga gcgggcaata aaaatgtcgc tactttgcac
    24541 acggttagag gttgcgccgg aggtcgactc gaaatgggcc acatcaacgg cgatagaagg
    24601 ggaacgctgt aagcgtgcgg cgtaaagctc atttaatcgt gccgtcagtt tttccgccag
    24661 cccccgcgaa ttagtaaaga caatggtcga gcgatggcgc aacacttcat caaggatacc
    24721 cgtttcaata tatggccaga tggagccttc ccggccggca tggctgtctt cgccagtgcc
    24781 gctggcgacc gatgagacat catccatatt ggcgaccggt acgacaatcc gtatctgcgg
    24841 atggcgcatt gcgggcgggt tgactaccgt aaccgggcga tcgccgccaa gaaatgctgc
    24901 cacatcgctg gctgagcgca cagtggcaga aaggccaatt cgctgtgctg aggtgtggag
    24961 cagcgcatcg agccgctcca gacttaacgc cagatgcgcg ccacgtttac tgctcgctac
    25021 cgcgtggact tcatcaataa ttaccgtttc gacgccgcgt agcgtttcgc gcgcgcggga
    25081 ggtcagcatc agatagagtg attcgggtgt ggtgatcaga atatccggcg gattacgggt
    25141 gagtttgctg cgttcctgtg caggcgtatc gccagtacgg atccccacgc gaagattgac
    25201 ttccgtttcg ccgcgccgcc gccgttcatc ggcaataccc tgtaacggga tctgcaagtt
    25261 gcgctgaacg tcggtaccca gggctttgat cggtgaaata tagaggatgc gtgaggtttt
    25321 acgtttatgc gcctcgcggg tatcttcgcc gccttcgcgg aagagctgat cgagggcgta
    25381 gagaaatgct gccagcgttt tcccggagcc agtcggtgca atcaccagcg catgttcgcc
    25441 tcgcgccgcc acatgccagg tttgcgactg gacagcggtc ggctgtttaa aggcgcgaag
    25501 aaaccagtcg cgggtcgccg gtgaaaacac gtccggcagg ggcgatgaag ggtttggatt
    25561 atctgccatt aacgctattc cccgtgaata tcatgcatga tgcgtggaat agcctcacta
    25621 tagatgcctg aatcattgca tgtcatcagg catcgactcg attacacctc ttcggcggca
    25681 ggtagcggcc agcctcccag acgtttccag cggttgacga tttcacaaaa cagcacagca
    25741 gtgcgttcgg tgtcgtacag cgcggagtgc gcctgggtgc tgtcgaagtc catgccagcg
    25801 gtctggcaag cctttgacaa tacggtttgt ccgagtgcca gcccggccag tgcagcagtg
    25861 tcaaaagtgg cgaaagggtg gaacgggtta cgtttcagtg aggcgcgttc tgcggcggcc
    25921 atcataaagc tgtgatcgaa attggcgttg tgcgccacca taatggcgcg gttacagccg
    25981 ctcgctttaa tacctttacg tacaacttta aaaatttcgt gcagcgcctc gtattcgctg
    26041 accgcgccgc gatcgggatc gttcgggtca atgccgttga aggcaagggc ttctggttgc
    26101 aaatttgcgc cgacaaatgg ttcgacgtgg aaatgtaatg tggtgtccgg catcagccag
    26161 ccttgttcat ccattttcag ggtgatggcg gcaatctcaa gcagcgcatc ggttttggcg
    26221 ttaaatccgg ctgtttcaac atcgatcaca acaggataaa aaccacgaaa acggtcacac
    26281 agaccggtaa gttgagcgtt atcggacatc tgattctctt aatacggata aattgcagcg
    26341 cgcattatga caaatattac ttgtcgatgc agtaaagatg cgggcgcgag gagttcacgc
    26401 ccggcaggag attagttgcc cagaccgcga ccggcgtctt tctcttcgat taactcaatt
    26461 ttgtaaccgt ccggatcttc cacaaacgcg ataaccgtag taccgccttt taccggaccc
    26521 gcttcacggg tcacgttacc cccgttttga cggatttttt cgcacgcttc agcggcatta
    26581 tctacgctaa gcgcgatgtg accataagca gtgccgagtt cgtatttatc cacgccccag
    26641 ttgtaggtca gttcaatcac cgcttcttcg gtttccgggc cgtagccaac aaacgccagt
    26701 gagtatttgt attccgggtt ttcgctggta cgcagcagtt tcatgcccag aactttggta
    26761 taaaaatcga tggagcgttg caaatcgcca acgcgcagca tggtatgaag aagacgcatc
    26821 tttttatcct caaaatggct aacagattca aaatgttgtt ttagtatagc ggcggcttta
    26881 cgccgctcgc aatgttggat tacaacgtcg ggtaatcggt atagccttcc gcgccgccac
    26941 cgtagaaact ttcggcacgt tgtgggttaa gctcagctct gcgctgcaag cgggcgacca
    27001 gatccgggtt cgcaatccag tcacgaccaa atgccaccgc atcaattaac cctttgccaa
    27061 tcagcgtttc ggctttttct accgtgtatg cacctgcgcc gataatcgga ccgtggaaac
    27121 gggcgcgtac tttttcgcgg aacgcatcag tatacggttc accccccgcc caatctggtt
    27181 ctgacatatg cagataagca atgccgcgtt tacccagttg ttcaatcaga tacagtgcat
    27241 cggcttcttc attcgggccg ttatccgtgt tctggaaagt accgattggc gaaacgcgaa
    27301 tgccaatgcg atcggcaccc cattcttcaa tcccggcatc gaccacttcc agtaccaaac
    27361 gtgcgcgatt ttccacgctg ccgccgtact gatcggtacg atggtttgaa gaaggggaaa
    27421 ggaactgatg cagcaaataa ccgtgagcag agtggagctc taccagatca aaaccggctt
    27481 cacgcgcgtt agcaatggcc tgacggaaat cattgacgat acctggaatc tcttccagtt
    27541 caagcgcacg cggcatggat gtttcaacac ggatcgcctg accattttca tcgcgcagag
    27601 aagtacgtgt tcccgcgcta agtgctgaag gcgctaccgg tgcctgaccg ccaggttgca
    27661 ggctggcgtg agaaatgcgt ccggtgtgcc acagctgtac ggccatatgc ccattttcag
    27721 catgaacgcc agcggtaatt tttttccatg cggcaatttg ttccggacta tggatgccag
    27781 gtgcaccagc ataccctttt gcctgggcag aaatttgcgt ggcttcgcta ataatcaaac
    27841 cggcactggc acgttggcga tagtattccg ccatcaacgg ggtagggatg tcacccggtt
    27901 caatactgcg cagacgcgtc agcggtgcca taaaaatacg gtttgccgca gttattgcgc
    27961 ccactttcag tggggaatac agtttttcag atgacataaa ggctcccggt cgtctggtga
    28021 taaaaaatgc ctgctaaacg gcaggcgtcg caataatggt ttttacatgt gccagcgcgt
    28081 tttccagtgg ctcggaattg cgcgaaattt tggcctgcag attcgcaccc agccatagtg
    28141 cgtaaagcac ttgtgcctgt tgcagcggtt cgccacaaaa ggttaaacaa tggctatcac
    28201 ggccattttc cagagcctgc gagagcaggg cgatcacgcc gcgagcgcct ttatccatcg
    28261 cgctacgcat atcttctgac agatcgcaca cttcggcaga gagttttact gtcaggcaac
    28321 cactgatggt tccatgttgg caaaactggt tcagtgtttg ctggtaataa gccagtatgc
    28381 ggtcgcggta gttaccttcg ccggattgca gcaactcagt cagtcgctgg tgatatgcgg
    28441 cgtaatggcg ctcaagcatg gctacgccaa acgcttcttt agagcgaaag tagtgataga
    28501 aggacccttt cggcacttca gcggttttta gtaattcgct tagccccatc ccggtgaatc
    28561 cacgttgcag gcaaagttgc tcgcccgtcg ccaggagatg ttcgcgagta tcatgttcgg
    28621 tgtgtttgtt catgcgttgg agtgtagtag accagtcggt ctagtgcaag caaagttgct
    28681 actttgggca ttaacagctt caataatggt aataatctta tttgaggagg gcgaagtggc
    28741 ggagcaatta gagttctttc ccgtccagag cccgtgccgg ggaatttgtc agtctgacga
    28801 acgcggattt tgtcgcggct gttttcgtag tcgggatgag cgttttaact ggaataaaat
    28861 gagcgatggc gaaaaacaag aggtgctgcg cctttgtcgg caacgattaa tgcgcaaatt
    28921 acgtgcaaat aagccagcgt catcagacga accggagcaa ccatcactct tttgagccta
    28981 atcttgcgta tactcctctc atatctgata agaggaagcg attatggttc agcgtattac
    29041 tattgcgccg caaggcccgg agttttcccg ttttgtgatg ggctactggc gattgatgga
    29101 ctggaacatg tccgcccgcc agctggtcag ttttattgaa gagcatctgg atctcggcgt
    29161 gaccaccgtg gaccatgctg atatttatgg tggctatcag tgcgaagcgg cgtttggcga
    29221 ggcgctgaaa ctggcacctc acctgcgtga gcggatggaa atcgtcagta aatgcggtat
    29281 cgcgacgacc gcgcgtgaag aaaacgtcat tggtcattac atcactgacc gcgatcacat
    29341 cattaagagc gccgaacagt cgctaattaa tcttgcgacc gatcatctgg atctgctgtt
    29401 aatccaccgc ccggacccgt taatggatgc cgatgaagtg gcggacgcgt tcaaacatct
    29461 gcatcagagc ggcaaagtgc gtcattttgg cgtatcgaac tttacgcctg cgcaatttgc
    29521 cctgttgcaa tcacgtctgc cgtttaccct tgccactaat caggtggaaa tatccccggt
    29581 acatcagccg ttactgctgg atggcacgct cgaccaactg caacaactgc gtgttcgtcc
    29641 gatggcgtgg tcctgccttg gtggtggtcg tctgtttaat gatgattatt tccagccgct
    29701 gcgtgatgaa ctggctgtgg tggcggagga gttaaacgcg ggctcgattg aacaggtggt
    29761 ttacgcctgg gtattacgtt taccgtcgca gccgctgcca attatcggct caggtaaaat
    29821 tgagcgcgta cgggcagctg tcgaagcaga aacactgaaa atgactcgtc aacaatggtt
    29881 tcgtatccgt aaagcggcac tggggtacga cgtaccgtaa gcacttttag gaatagccgc
    29941 cgttcaaaaa tgtgtcactg gtttacactt attcaggaat gcacaatgaa cggaggtcct
    30001 atgaaacgtt ttagtctggc tattctggcg ctggttgttg caaccggcgc acaagctgcc
    30061 agtgaaaaag tcgagatgaa cctcgtcacg tcgcaagggg ttgggcagtc aattggtagc
    30121 gtcaccatta ctgaaaccga taaaggtctg gagttttcgc ccgatctgaa agcattaccc
    30181 cccggtgaac atggcttcca tattcatgcc aaaggaagct gccagccagc caccaaagat
    30241 ggcaaagcca gcgccgcgga atccgcaggc gggcatcttg atccacaaaa taccggcaaa
    30301 catgaagggc cagaaggtgc cgggcattta ggcgatctgc ctgcactggt agtcaataat
    30361 gacggcaaag ctaccgatgc cgtcatcgcg cctcgtctga aatcactgga tgaaatcaaa
    30421 gacaaagcgc tgatggtcca cgttggcggc gataatatgt ccgatcaacc taaaccgctg
    30481 ggcggtggcg gtgaacgcta tgcctgtggt gtaattaagt aattaagagg ccagcgtacc
    30541 ttgcggtggt gcttgctcaa gttgcgaaag cgagcagtac agccgccaga caattgccgc
    30601 cagctcacgg gcggcaggtt gatgatgacg ggcaaggctg tcgcaaatcc gctgtaattc
    30661 ttcaagtgtg gccgccagtg atttttgctg aacgccacgc tcactcatca ctccccgcaa
    30721 cagtgaaata cagttatccc gtactcgcga taacggatcg gaacgcgatt cccagtcgcg
    30781 taattgccag acaacatgag aacagttcag cagcactaca ccccagcgta ataaccaacg
    30841 gcgagccagc gcatcctggc tgttactcaa ctgactgacg tgatgataag tgagcgattc
    30901 aaattcgctt tcactcagtg ttggatggcg gcttagttga tcgacaaaat cccgacgcag
    30961 cgcgcgaata tggcgacggc ttttacgagc atccgatcct ggacgcagaa tggcgaacgc
    31021 taaccacgcc aacgcgacgc caacgatttt cgccagatta tcgttaagaa aatcagcaaa
    31081 atcatacacc gggggattag tgacagcgat aaacgaaccc ataaaaacaa tcagttgccc
    31141 ccacagtgcg gcaaattttg gcatctgcaa tttaagaagc tgcatcgtcg ccagcagtgg
    31201 aaagagaaac agcaaaaatt gccacagatc actaatctgg accatcaggc caaatttgac
    31261 cacaaagctg aatagcgaaa gtaacaccag cgtgcgcatc agcagtgaca acgacttaaa
    31321 cggtgctgcg acggcggagt aaagtacgca gctaattgct gccagcgtta atgcattggc
    31381 accggcatcc cattgcgaag caatactcca tgcgccaatc atcatcaacg tacaaaatgt
    31441 acgcagcccg ctccacatag cttcggcgtt atcggtatga cgagccaggc cggatgtccg
    31501 tgggagtctg gtatgatcat ctacaccgct ttgcagacga tgtaattcct gactactttg
    31561 cagataaagg cggcaaaaat aacgtagtcg ctgccagaag gcgacgtgcc gatagtcggc
    31621 gacgttggtc gggcgtagcg gggcgataat acgtgcgacg gtgtaaacat ctgtttgcga
    31681 actggcgagc gccgtcagca actgttcgag aatttctcgt gtggcacctg gcggtgaggg
    31741 ccagttgagc aacatacgtc gcaggctgga gatgacactg gtcatacgta attgctggtg
    31801 gagcagcgca ttaaggcgcg cgttttgctg gcgaaaacga tagtggctcc agaaagcctg
    31861 gatacgcagc aaattcatgg tcagtatctg cccaatcacc ccttcatgtg ctgcacgaat
    31921 ggcatcggtt gtttcaggct gccagagtaa actggcatgt tcaagtagtc gggcgtgcat
    31981 gtttttcaat gcggttaaaa gggctgtagc atcggaactg ctaggcagga tcatcatcat
    32041 catgccgccg cacaaaatac cgacaatcac ctcgcaaacg cgcgcctgag cgatatccca
    32101 cagctggctg gcctcagtaa tattaaccat cggaaaggcg atgatggcag ccgtgtagcc
    32161 cgccagttga aatgcatacg cgacgttatt cgtgaagtgc gcacaggccc aggtacaaaa
    32221 gccaagccac gccgacatgc tcaatagaaa aaaccacggc tcattgagcg tatgtccggc
    32281 aagaagcagt gccgcaatgg ctcccagtaa actgccagcg atgcgtccga ggcttttgct
    32341 gatgacaccg ccaacggtgg gaaagctaac cactgcagcc gaggtcatcg cccaataggg
    32401 ttcatccaga ttcaaataat aggcaaccgt cagcgccaga cacatagcaa tggtattgcg
    32461 taacgcataa cgccattgcg ccagcgtggc cctgaaccag ggcaaattgc gcaaggacca
    32521 tgacgatgcg ttcattattg acctacggca atgctacagg tggtgcccga caccagcgtg
    32581 acatcgcctg gaactttatc cagcgcaaag cggacgggaa cgcgttgggc gagacgaacc
    32641 caggggacgt taggtttcac atccggaata aggcttgagt cactttcaac gctctgatca
    32701 taaatcgccc gaccgatact ggaaacgtga ccctgtaacg ttttattatc actatagagg
    32761 gtaatttgtg ctggtgcacc ttcgcggata tggcgcaact tggtttcttc aaaataacca
    32821 atgacgtaaa acgagttgct gtcgacaagg gcaaacagcg gtttcccggt atcagcgtaa
    32881 tcgccgatgc gggtagtaag gttagtcacc catccactca ccggggcgcg aatttccgtt
    32941 tgcgccagtt gccattgtgc ctgtttcagc gtcgcctgtg cggcatcaac actggcctgc
    33001 atcgctttaa cattgaggtt agcggtatcg agttcttcgg cagaaataaa attttgtgag
    33061 agatgacgac gacgattcgc ttcgttgttg gcttttgcga ggtcagattg cgctttcgcc
    33121 agctgagcct gtgcgttcag ctcggcgatc tgaaagggcg ttttatcgat cgtgagcaga
    33181 agatcccccg cgttaaccag ctggttgtct tttatattca gctcaacaat acgtcccgac
    33241 acctgtgggg tgatagaaac ctgttctgcg cgtattttcc cgtcgcgtgt ccacggagat
    33301 tgcatgtaat aattccacag ccaccatccg gcaagaaccg caactatcgc tacaatgatt
    33361 gttgagaaat acttaattgt tttaattgac atagttacca cgcaatcagt attgccagag
    33421 caaggcaaac gcaaatcgca aacagcgata aatccattaa caagggatgc cagatgtcac
    33481 cggcgtagat ccagccacga agcaggcggt gtacgacaag ccagatgacg aatccaaacg
    33541 cgaaggcttt gaaaaacgga ggaaagtaga cggacgcacc gaacaccaga tcttgtaagg
    33601 gcaatcctgt tgcattgagc ataaacttca caaagtagat tcctttacga ccgaatatat
    33661 tgcgttagat taataaatat tctttaagtg cgaaaaattt acgcgcaatt tctgaaaaat
    33721 atgtctgacg gtaaccaaat gcagcaatac atttgtttta gcaatacaat tgctgcacac
    33781 tattctaaaa gccgcataat atcttagcaa gctaattata aggagatgaa attggaatcg
    33841 ccactaggtt ctgatctggc acggttggtg cgcatatggc gtgctctgat agaccatcgc
    33901 ctgaaaccgc tggagttaac acaaacccat tgggttacgt tacacaatat ccatcagtta
    33961 cctccagacc agtcgcaaat tcaactggca aaagcgattg gcatcgagca gccatcactg
    34021 gtccgtactc tggaccaact ggaagaaaaa gggttaattt cgcgtcaaac ttgtgccagc
    34081 gatcgtcggg ctaaacgtat taaactgacg gaaaaggcag agccgctgat cagcgaaatg
    34141 gaagctgtta ttaacaaaac ccgcgcggaa attttacatg gcatctccgc agaggaactg
    34201 gaacaactga ttacgctcat cgcaaaactt gagcataata tcattgagtt acaggccaaa
    34261 gggtgaaatg aagggggcgt gtggtcagta acctgaccac acgcaaacta tttagcgcgg
    34321 agaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg gagagaaacg
    34381 agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac gaatttccag
    34441 ctcgacacct tgcgttttgt tcattgcact ctgtacgccc tgaccagcta cgccacctgc
    34501 aacagcgcct gctgcagtag ccagagaacg cccggttccg ccaccaacag tatttcccag
    34561 gaaaccacca agaacagcac cgccaattgc accgatcacg ttggaatcat caccgccctg
    34621 aatctgtacc ggacgtacgt taacgatggt tccatagctg acattctgta cttgtttcgc
    34681 ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga
    34741 cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca
    34801 tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt
    34861 acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa
    34921 aacattcatg atgattattg atttgaagat aatgttttac taccaacggt aaaaatcggt
    34981 cattccgcat ggtattcacg aaaagtttat gttggagtgg tgcgcaaacg tcagattgaa
    35041 aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac cagccttgat
    35101 ggtgttgatg ttgtgttggc gacaattgat gaacaccggg tcgcacagct ggcaagtttg
    35161 agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca gggccagcag
    35221 cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt tgctgatgcg
    35281 gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc gatcggctgt
    35341 cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt acagattggc
    35401 gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt tcgccgtcgc
    35461 gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca tgccttgctg
    35521 gctcacccaa ccgagcgacg aatggtgctc aatattggcg gcatcgccaa tctgtcactg
    35581 ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat gctgatggat
    35641 gcctggatct ggcgtcaggt cggtaaacct tacgataaag atgccgagtg ggcacgggcg
    35701 ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt ctcgcaacct
    35761 gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg ccatttacgc
    35821 cattttccgg gtgttgatcc ccgtgatgtg caggccacac tggcagaact caccgccgtg
    35881 accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt atgtggtgga
    35941 ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg cacagaagtc
    36001 accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc tttcgcctgg
    36061 cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac tggcgcaagc
    36121 caggagacgg tactgggggc tattttcccc gctaacccgt gatatcagag ttaactgaat
    36181 ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt gccctccaga
    36241 ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta ctgatctgtt tgccggtgct
    36301 tctcaccggc tgtagcgcgt tcaatcaact ggttgagcgc atgcaaaccg atacgctgga
    36361 ataccagtgt gatgaaaaac cgttgacggt aaaactgaat aatccgcgcc aggaagtcag
    36421 ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcct ctggtgcacg
    36481 ttacactgac ggaatctatg ttttctggtc gaaaggcgag gaagcgactg tctataaacg
    36541 cgaccgcatc gtcttgaata actgtcagtt acaaaattca cagcgttgag atttttccag
    36601 gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg tctgataacg
    36661 acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg ttacgccgcc
    36721 gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag gcttgtgaag
    36781 ccaaactggc ggaccctacc gcgatggtgg tcgctaccgt ggatgaacat ggtcagcctt
    36841 atcagcgcat cgttttactc aaacattacg acgaaaaagg catggtgttt tacaccaacc
    36901 tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg ctgttcccgt
    36961 ggcataccct tgaacgccag gtgatggtga tcggtaaagc agaacgactt tcgactctcg
    37021 aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca tgggtttcga
    37081 agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg gagctgaagc
    37141 agaagtttca acagggcgaa gtgccattgc cgagcttttg gggcggtttt cgcgtcagcc
    37201 ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc tttttgtacc
    37261 agcgtgaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
    37321 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
    37381 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt tgatggcaag
    37441 cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga cggacgagga
    37501 agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct tcgatcctac
    37561 cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac gcttccagca
    37621 ggcgggccac aagccggttg cgctggtagg cggcgcaacg ggtctgattg gcgacccgag
    37681 cttcaaagct gccgagcgta agctgaacac cgaagaaact gttcaggagt gggtggacaa
    37741 aatccgcaag caggttgccc cgttcctcga tttcgactgt ggagaaaact ctgctatcgc
    37801 ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc gcgatattgg
    37861 caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc gtctcaaccg
    37921 tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg gttatgactt
    37981 cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg accagtgggg
    38041 taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg tgtttggcct
    38101 gaccgttccg ctgatcacca aagcagacgg caccaaattt ggtaaaactg aaggcggcgc
    38161 agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct ggatcaacac
    38221 tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca ttgaagagat
    38281 caacgccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc agtatgtact
    38341 ggcggagcag gtgacccgtc tggttcacgg tgaagaaggt ttgcaggcgg caaaacgtat
    38401 taccgaatgc ctgttcagcg gttccttgag tgcgctgagt gaagcggact tcgaacagct
    38461 ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcgct
    38521 ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg cctccaatgc
    38581 catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg
    38641 tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc tgatttgctg
    38701 gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag
    38761 ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta
    38821 acagtgcggc agagtttccg atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg
    38881 ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg ccgcccagcc
    38941 atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc tgtgatgccg
    39001 tattaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt atcgtccgtc
    39061 aggtgaaagc agcgaatccg caggcgaaat atttttgcga tccggtaatg ggtcatccgg
    39121 aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac ggtttgcctg
    39181 ccagcgatat cattgcgccg aatcttgttg agctggaaat actctgtgag catccggtaa
    39241 ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg ccacaaattg
    39301 tattggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa atgttgctgg
    39361 tcaccgccga tgaagcctgg catatcagcc gtccactggt ggattttggt atgcgccagc
    39421 cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt cagggggcaa
    39481 cgctgcagga ggccctggaa catgtgaccg ctgcagtata cgaaatcatg gtgaccacca
    39541 aagcaatgca ggaatatgag ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag
    39601 aacattactt cagcgcaaca aagctctgaa atatctcatt tcggcccaca acgacctgtg
    39661 ggccgatggc tattacttta agctttccgc tgacagcgcg tcttgtactt ccggacgttc
    39721 agccatacgt tgcataaatg ccgcaatgtg ctctaaccct tccagattca gtttcaccgc
    39781 gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc tttgcccgca
    39841 gatccagtgc tcatccttca gtgcctcgtt cacatattgc agcttcttct ccagttgcgc
    39901 gcgaactgtc ggtttgtact cttccggtgt atcagggcga aacagaggtg tgaaaccttt
    39961 atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg aaatactgtt
    40021 taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca taatcgcaac
    40081 gccttccgtc agcaaagtac catcgtccag cagcaatgca ggcacctgcc ccttagggtt
    40141 aacggcaaag taatcgtcac cgttttcgag acgttttttc attaaatcca cactgacgag
    40201 cgtaaaatcc tttccgctct cacgcaggat gatatgggaa gcgagagagc aggcgcccgg
    40261 tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt
    40321 tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga gtttccctga
    40381 attttacgct acggctgctt tcgccgcttt gtctgcagcg tcatcctgcg tcatgcggtg
    40441 cagtttcggc gcggtcagca gcatcagtac tgcaataacg gcagtagcga caccaatctg
    40501 caagaatacg cgaccataga cttccagtga catcagcgga tcggtaacgt tatccggcac
    40561 agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg tcaggaacca
    40621 gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca ttgccagacc
    40681 cagaccagag atcatcagtt ccccgatact ctgcaggcca tagctcgcga ccagccagct
    40741 tacagacacg atacctgcgt cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc
    40801 accagaacac atcaccatac cgattgcaaa cttggttggc atcggcaggg tatcgcccat
    40861 cttgttatag atagcggcca gaatcgggct accaatgatg atccagaacg ggttcagtgc
    40921 ctgatactgc tcaggttcta cggccaggcc cagaatggtg tgttcaacgt tacgaatcgc
    40981 aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa tggcttcgag
    41041 catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg cgaaggcttc
    41101 tttaccgaag ataaccacga taccgaaggc aacaacgccc agcgccatac gcgcaacttc
    41161 ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa taatggtcag
    41221 cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact gtttaaccca
    41281 gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc tcaatgcaaa
    41341 cgcaacgctc cagccgtatt tcgcggccag ccacggcgta gcaatcatag agaagaaaga
    41401 gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgtg ggtcgttttt
    41461 ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt taccgaccgc
    41521 aatagccgcc atacccatat aaacaatacc ggcgtcgtga ccagaccagg caaccagagc
    41581 ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac ccagtacctt
    41641 gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa aggaagagaa
    41701 aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa cagccataat
    41761 tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga tgagatagaa
    41821 cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt ggtttgcagt
    41881 ggacactttt acctcttttt ttacatccca tattaacggg ggtgctttct cgcgttatgt
    41941 ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa tgttcactat
    42001 cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag gcggggaatt
    42061 caaccattcc aggaaatgtt atccagcgtt aaatttttca aaaaacgtaa cggcagattt
    42121 tttcactgca ttgagagggg gatgatggct tgacggcgat atgttctgct atttagcgtt
    42181 tttcctagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt gtaggtcaat
    42241 atttgttgcg tgtgtaaatt taaatgattt taacctgatt cttgcaatat ttttacattg
    42301 ccacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa aaaaacaatt
    42361 aacctgcatt tttggttatc cgcaaacgga ttacctgatg ataacgctaa gcaatggcat
    42421 cattgacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat ggggtaatcg
    42481 gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga tcttcaataa
    42541 tacaagagcc acagcggggc ttgcgggcaa tgcaggtata acgcccgtgc aggatcaacc
    42601 aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt tcttctacct
    42661 gttcgacgtt tttccccggc gcaaattgag tacgattaca aacgcggaaa atgtgcgtgt
    42721 cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct gttttacgac
    42781 ctacgccggg cagggcttca agcgcagcac gatcttccgg aacctcgcca ttatgctgct
    42841 ccagtaagat acggcaggtt ttgatgatat tttctgcttt gctgttataa agcccaatcg
    42901 ttttgatata ggttttcacc ccttcaatgc ccagttcaag catcgctgca ggcgtattcg
    42961 ccaccgggta gagttttgct gtcgccttat taacactgac atcggtcgcc tgagcggaaa
    43021 gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag
    43081 gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc atcagacatt
    43141 ccctgtttca ccgtttggca atgcacgttc ggcagctgct tcagtacggc gctttttcat
    43201 tctttcatca atcaggtatt ttcctgccag catcagtccc aggccaataa atgcacctgg
    43261 tggcagcatc gccaacagga aaggggagtc ggtgtggaaa atctccacgc gtaatacttt
    43321 tgcccagcta cctaacagcg catctgcacc gtcaaacaat gtgccattgc cgataatttc
    43381 gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg aaaagccgtc
    43441 cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc ccacaacgat
    43501 acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc caaaggcgta
    43561 ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca cgtaaatggg
    43621 aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaggt tggtcagcgt
    43681 cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg acgtgaccgc
    43741 caacagagga caaaggccga gcaactggac cagcgcagag ttgtttttcc acaacccctg
    43801 aacaataacg tctttaattt cgctcacgct ttattctcca caggcaggaa gttgagaaag
    43861 ttgtgccggt aacgtctgag cgtacaaccc ggcgcgtttt accgcattaa ccaccgcgcg
    43921 gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct tcaccgccca
    43981 gtgcgcatca tccgcaccac tgattttttt acccgcaaaa tgggtgatcc agtcagaaag
    44041 gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca cgcgcgtgcc
    44101 aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg aatagccatc
    44161 tggcgcggtt gcttccagaa cggcggctac cggtttgtca tcctgtttgg cgatgtaaac
    44221 ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct gtgccagcgc
    44281 attgttatag cgttcggctg gcagcacctg atcaaataac gccttttgtt gcagactggc
    44341 ctgttcagca atcgtcgttt tggtcatctg gttgatggct gcagttaacc ctgttgaacc
    44401 cgctgcaaat agcgccaacg taatgccgtg ttttcggata gttttcagca tggtttaccc
    44461 tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt
    44521 cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc ggatcaacca
    44581 gactaataag cccgcaagcg cgccgaaaat aagacgacca cgattggtcg tagaagcggt
    44641 aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc gctccagaca gcagatgaat
    44701 ttgcggtgct gccagtgttt ctggtgagaa caaccagccc aacgttgcgc ataacgccag
    44761 cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata gcaaccatac
    44821 gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca gaataccgct
    44881 gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag aggttttaaa
    44941 tgtatccagc ggtgtcgcct gactaatgcc atcaatacct aagcgtagcg tgttcatatc
    45001 accaccactg gcggtatgac cgctgaaaat aacctggata gcgtcgataa aaccagggat
    45061 gttgaccgca atttcatgtg gtggtaacca gctggtcatc tgcaccggga aggagatcag
    45121 taagaccaca taaccaatca ttgccggatt aaacgggttt tgtcccagac cgccatataa
    45181 ctgtttagcg atgatcaccg caaacaccgt acccagcacg accatccacc atggcgcgag
    45241 ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat ctttcaacgt
    45301 ggcggctacc gactgtttgc gtagtttgag tacgagagct tcggctaaca gagcactgac
    45361 cgacgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt gcgctgcgat
    45421 tcctggcacg gctgcgagca acactaacag cataatgcgc gatgtctggc gctggttatg
    45481 ggtataagga gagctagcta ttctgaatac catttagtcc tcgtttacaa ccttttgctg
    45541 ggcggctttt ttggcctgaa cgcgggcaat agccgcggca actgccgctt tgcgcggatc
    45601 aacctgttct tctggtaccg cattagcctg ttgctgttcc agcttgcgtg ctttggcgcg
    45661 tgcaatagcc gcttcgacgg cagctttgcg cggatcgacc tgttcttctg gttccgcatt
    45721 agcctgttgc tgttccagct tgcgtgcttt ggcgcgggca atagcggctt cggcggcggc
    45781 tttgcgcgga tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg
    45841 cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga cctgttgttc
    45901 tggctccgca ttggcctgtt gctgttccag cttgcgtgct ttggcacggg caatagcggc
    45961 ttcgacggcg gctttgcgcg gatcgacctg ttgttctggt tccgcattag cctgttgctg
    46021 ttccagcttg cgcgctttgg cgcgggcgat agctgcttca acggcagttt tacgtggatc
    46081 agcaacggtt gctgcgtcgt tagtttgctg cagttctgcc tgtttcgctc tggcttgcgc
    46141 tttacgggct tcccgtgctg caataattgc actgttatcc gggcgttcgc ccgctttaat
    46201 cacaataggc tgtgtagcct gggcctgttt ctctttcacc cgcgccagag cggcagcaat
    46261 cgcatcttta tctttggctg caggttgaac ggctgcgctc ttatgtcgtt caaggcgagc
    46321 cgctttttcg cgctccagac gagcctggcg cgcttcgaaa cgcgctttgg cttctgcggc
    46381 gcgcttttct tcctgacgaa tagccgcaat ttcagctttt tcctgacgga aatattgcac
    46441 caggggaata ttgctcgggc aaacccacgc gcaagcccca cattcaatgc agtcggcaat
    46501 gttatgcgtg gtagctttat cgtgttgctg acctttgctg aaccagtaca actgttgcgg
    46561 caaaagatcc gcagggcagg cgtcagcaca ggcgctacac cggatgcagc tttgttcttc
    46621 ctgtggttcg ccaagctcat tggcagaggg agccaacaga cagttggtaa tctttacgac
    46681 cgggacatcc agccatggca aggtaaagcc cattagcggg ccgcccataa tcaccatttg
    46741 atcggcagag gggcagaatc cggcgtcatt caataaatga cgcactggcg tccccagccg
    46801 tgcccagacg ttgcccgggc gagcgattgc ttcgccagtc agggttacga cacgctcggt
    46861 aatcggctcg ccatcaataa cggcacgttt cactgcataa gcagtgccga cgttttgcat
    46921 taatacgccg atatcggatg aacgcccgcc atgtggaacc tgcttcccgg tcagaatgta
    46981 ggttaattgt ttagcaccgc cagaaggata tttggttgga atcacccgca gagaaatatc
    47041 gtgagagtcc gccagcaccg cgcgcagcat ggaaatcgcc tgcggtttgt tatcttcaat
    47101 gccgataaga atttcgcgtg gctgcagaat atgcgcaaga atgcgaatac cctctacgac
    47161 ctgagccgcg caatcctgca tcaaacggtc atcggcggta atgtacggct cgcactcagc
    47221 agcgttgata atcaacgtct caatcttatc tccgccgccc tgcaatttaa cgcctgtcgg
    47281 gaatcctgca ccacccagcc ccgcaacacc aaactgatga atgcgctcga ttaactcttc
    47341 gcgacggcga gagcgataat cggcccagcc gtctcgtggg atccagcagt cttcaccatc
    47401 ggcatcaata atcacgctta attcagctaa agctgaagga tgagccgtag agtggggcgc
    47461 aatagccgta acggtacccg aggtgggcgc gtgaacaggc agcattttgc cgcgaccacg
    47521 ggtaagcggc tggccgcgca atactttatc gccgacgcta acgcacaact caccttcagc
    47581 gccaatatgc tgtttcagtg gaatgacaaa acgctgcgcc agtggtacct ggcgcagggg
    47641 ggtaccgttg gactgggttt tcatctccgg tggatggatg ccgccgttga aatcccagat
    47701 tttatttttt ctgaatgcag agaataactt aagcatggtg ttccacggga atgatacgca
    47761 cgggaatggt gttcagatcc catttccagg agtcaggtgt ttctgcgacc ggttgcaacg
    47821 agatgcagtg cgtcgggcac ggatcaacac ataaattgca gcccgtacag agatcactca
    47881 ttaccgtatg catggcacgg gtagcgccaa cgatggcgtc taccggacac gcctgaatac
    47941 atttagtgca gccaatacag ttattttcat caataaccgc caccatccgc gcaggcgtta
    48001 gctcttgcgc ttcgccatcc agtggctgcg gctcgacatt aagcaactcg gcaattttta
    48061 gcatcacagc ttcgccacca ggggcgcaac ggttgatttt ttcaccgtta cagctgatgg
    48121 cttccgcgta ggggcgacag ccgggataac cgcactgacc acactggctc tgcggtaaga
    48181 tttcgtcaat tttttcaacg accggatcgt cttccaccgc aaaacggcgg gaggcataac
    48241 ccagaatggc gccaaacgcc aggcccagca ggctcacggc ggcaacggca atccagatag
    48301 cattcattac aacttcacca aaccactaaa gcccataaag gccagagaca taagacctgc
    48361 ggtaattaac gcaatggcat taccgcgaaa aggagccggg acatcagcca cagcaaggcg
    48421 ttcgcggatg gctgcgaaga gaaccatcac cagcgagaaa ccgacagcgg cggaaaaacc
    48481 gtacagcgcc gactgcaaga aattgtgccc gagattgata ttcagcaacg ccacgccgag
    48541 cactgcacag ttggtggtga taagcggcaa aaaaatcccc agcagacggt aaagcaccgg
    48601 gctggttttg cgcaccacca tctcggtgaa ctgcacgacc acagcaatca ccagaataaa
    48661 cgccagggta cgcaggtaaa tcagattaag tgggatcaaa atccacgtat cgataagcca
    48721 ggcgcaaata gacgccagcg tcatcacaaa cgttgttgcc agccccatgc ccatcgcggt
    48781 ttccagcttt ttggaaaccc ccataaacgg acagagaccg agaaacttga ccagtacaaa
    48841 gttattgacc agtacagttc cgacaaacag taacaggtag tcagtcattt ttcggcctga
    48901 aataaaaaag ccgcctatta tccgttaatc cagagcaggc gacaacaggt taactgtaag
    48961 gttattacgg attcacaaac gtcgctttca cccgcgacga gcgtttgaaa taggggacga
    49021 tcaatgcggt tgccaacaaa gtaaacagca actggcgaac ggctatgcca tcttccacgg
    49081 gtgaaaaagc gaacgctttt actgccagca atacggaaat aagcagccag ataatatagt
    49141 gtttaggaac acaacgccga cgtttaaaga aagcgatggt caaccacagt gtgtaatacc
    49201 acatggcgat agcggtaata aaagaaacgc cccataagat ttgtgtcgtc agtgcttgtg
    49261 cgccgagcgt ttgaaatgtt tgcggagaag ataacgcagc ggtatacagc aacaacgcca
    49321 gcgtcgtact taacagagcg actaaaagcc aggccaacgg gccaagcaac cagcctccaa
    49381 tacgttgtgg cgttgtggtg gtcataggtt ctccagaata gcgacgcaaa caggcaaatc
    49441 taattttagg ggcgttatta taaggctttt ataaccgatc gctactttct ttattgaaca
    49501 tagtgccaga cggacttcgg tacctggccg aggtcaaaca aacgcccgcc agaaaccagc
    49561 tctgcgcgac ggtgatcggc agcacgatac atattgatca gttcctgatc gtccgtcagg
    49621 gtgtaattaa gatggtcgta gagtttttcc agactttcga gtgaactgat tttgcgaaat
    49681 tttaataagt agtcctgaac agtcataaaa ggtccataaa ataattaata ccaaaggggt
    49741 aggtattatt agtgatgata aaatttcacc aaaagtgaac gcttgtgtta ttacaataat
    49801 aagaatgcgc tgatggcaaa tattcaggga tgaaaaactg ctatgaaaag agtaacgcgc
    49861 tttactatcc ctgacaatca ccaacaacac cgaacaagat aataaattcc tggtttaata
    49921 tccgacaagt gaaaacatgc accctgacgg gcagcatgtc gctctacaag cgcagagctt
    49981 acttgtgttg taccgtagta ctctgttcag gtggctgata gttgtcaatg tgactcgcca
    50041 cgccaagaag aatgactgaa acgacaagaa cgatccaacc tgttaattca ataagacgat
    50101 tcattacaac tcacatctct gttgattgat ccattaactt cagggggtaa atgttactta
    50161 gcaataatag ctccagcaag atttttactg aggtttttgc gatatttagc ttttgtcgtt
    50221 ggaaaaatcg ctattttttt gacttaagtt aaacaacatc ccttattgct ggcaggttat
    50281 taaactgttg agcgagggta aaggatagtg tcaaatagcc atcatacttc aatgagaggc
    50341 aatgacatga gcgacaacat ccgtgttggg ttgattgggt atggttatgc gagcaaaacc
    50401 ttccatgcgc ccctgattgc gggcacgccc gggctggaac tggcggtaat ctccagcagt
    50461 gatgaaacaa aagtaaaagc cgactggcca acggttacgg ttgtctctga gccgaagcat
    50521 ctgtttaacg atcccaacat agacctgatt gtcattccta cacccaacga tacccatttc
    50581 ccgttagcca aagcggcgct tgaggcgggt aaacatgtgg tcgttgataa accctttacc
    50641 gtgacactgt cacaagcgcg agagctggat gcgttggcaa aaagcctggg gcgtgtgctg
    50701 tctgtattcc ataaccgtcg ttgggatagc gatttcttga cgctaaaaga tttgctcgcg
    50761 gaaggcgtgc tgggtgaagt tgcttacttt gagtctcatt ttgaccgctt ccgtccgcag
    50821 gtgcgcgatc gttggcgtga acagggcggc ccaggcagcg gtatctggta cgatttagca
    50881 ccacatcttc ttgatcaggc cattacgcta tttggtttac cggtcagcat gacggtagat
    50941 ttggcacagt tacggcccgg agcgcagtcg accgattatt tccacgccat cttgtcctat
    51001 ccacagcggc gagtcatttt acacggtacc atgctggcag ccgctgagtc agcacggtat
    51061 atcgtgcatg gatcccgagg cagttatgtg aaatatggcc tcgatccaca ggaagaacgt
    51121 ctgaaaaatg gcgagcgtct gccgcaggaa gactggggct acgatatgcg tgatggcgta
    51181 cttacccgcg tggaaggtga ggaacgtgtc gaagaaacgc tgttgacggt gcctgggaat
    51241 tatccggctt actatgcggc gattcgtgat gcgttaaatg gcgatggtga aaatccggtt
    51301 ccggcaagcc aggcaatcca ggtaatggag ttgattgagc tgggcatcga atccgccaaa
    51361 catcgcgcga ctttgtgcct tgcatgaaaa atggatggtg caatttgcac catccatttt
    51421 tgttacttcg ctgcgacttt ttctcgcagt gcgcgttttt cctctgcgtt gaggaaagcc
    51481 atttccagac cattaatctg tgcctggcgg atttgctcgc gggataaccc agcagctggc
    51541 gcggcaacgg tatattcgtg aatgatatcc actccctgta cgccgggatc gtcagtgtta
    51601 atgctggcac gaatgccatg ctcaaggaac gttttcagcg gatgtgcagc cagctctgcc
    51661 acggtgctgg tctgaatatt ggaggtcaga caggattcaa taccaatttg ttgctgggcg
    51721 agaaaatcca tcagcgcccg atcttcaatg gcttttacgc catgtccaat gcgctctgcc
    51781 cccagttcac gaatcgcctg ccagatgctt tccggcccgg cagcttcgcc tgcatggacg
    51841 gtaatatgcc agcccgcatc acgcgcgcgg ttgaagtgag aaaggaacag acttcccggg
    51901 aaaccaagtt catcaccggc taaatcaagt gcggtaatct ggtcacggtg ggctaaaaag
    51961 gcctccagct cttgctgaca ggcggcttcg ccgaaggtcc ggctcataat gccgataagc
    52021 ttcgcctgca caccaaaggt gcggcaacct tcacgtacgc catcgatcac cgcttcgaca
    52081 acacccgcta caggcagctt atgtgccatt gccatgtagc ctggtgagaa acgcagctcg
    52141 acatagtgca ggccgtgacg ggctgcatct tcaatgtttt caaatgctac gcggcggcag
    52201 gcatcaagag aggcgagaac tttaacaccc cagtcaagtt tggtcagaaa gctcaccaga
    52261 tcgggttcgt tggcaatgac ctgaacgtgg ggaatcagtg tttccaggga ttgtgcagga
    52321 agcgagatat tatactggcg gccaagttca agaatggtct ggggacgaat gttgccatca
    52381 aggtggcgat ggatatcagt taatggcagg gtggtatcaa tcatggtcgc actctttttc
    52441 tggatataaa agtgcgcacc attataaaaa caaagcaggg taaaaatcca cgattgcgca
    52501 acgatttttt acgttgcgca attggggtta acgaacagcg cggatggcgt taattaatcc
    52561 agccacacct ttttccagtt tcgaacgtgg gcagccggca ttgagacgga caaaaccacg
    52621 accttcttca ccgtaggtat accccggcat gatcgcgact ttttcttgtt cgataagtgc
    52681 tttttgcaac gcgttgtcgt caatattcaa cggacgtaaa tcaagccagg ccagataagt
    52741 ggattgcggg atctgccagt tgagttcagg aaacgcggcg ttcattttat ctgcgatata
    52801 cgtcaggtta tctttcagat agacgcgtaa ggcatccagc caaggcgcgc cttgctgata
    52861 ggcggcgata tgagcagtta acgccagtac cgaaggggaa gaaagcccat cacgtccttt
    52921 cagtgccgat aaataggcat cacggctgct gctattttct ataatcccgt aagcaccggt
    52981 cagcgcggga atattgaaac ttttcgatcc cgacgtcagc aacgcccagt ctccccgcgc
    53041 cacattactc cagggaatat gcggctgctc gccccagacc atatccatat ggatttcatc
    53101 ggaaataacc cgcacaccat gacgctcgca caggtcagcc atgatctcca gctcatcgca
    53161 cgtccacact ttcccggtag gattctgtgg gctacacagg agcataattt tacattctgg
    53221 tttcgccaac acggcttcca gcttgcccat atcgcaaaac cagccgtcag cctgcttctc
    53281 taaagcaacg ggcattactg tgcgctgatt accttcaatg gccttgtaaa atgcgtcata
    53341 ggcgggtgtg tggatcacca cgccttcacc tgtttcagac cactgacgaa tcagttctga
    53401 gaccatatag ataacagaag gaccatacac caccgactga ggatcgatgg cggtgtaatg
    53461 ctgggtggaa aaccagtggg caatagccgc aagaaactca tcgtttttcc agcggctgta
    53521 gccaaacacg ccgtgcatca ggcgctgatt cagcgcctcg ataatgcagg gggcagtggc
    53581 aaaatccatg tctgaaatcg tgaacggtaa caggtcagca gtgccgaaac ggtcagcgac
    53641 ataatcccac tgtgtacacc atgtgccatg acgatccacg acctttgaaa aatcgaacat
    53701 atcttatcct tatgcctgga cagtatgcat cagaccggcc atttcatctt ttactgactg
    53761 aacttgtggc ccgataacaa cctgcaggtt atgttgatta agttgtacta cgccaattgc
    53821 ccgattgtcc ttcagtgcct gcacattaac aagcgacata tctttaacag acaaacgcag
    53881 acgggtaatg cagttatcga ggctgacaat attgtcggca ccgcctaagg cttcgaggat
    53941 tgccggaacg ttgtaacctg atttacccgg cgcaccggca acggcttttt cgattgaact
    54001 ggccacttcg ctatcgcgcc ccggggtttt cagattgaag cgggtgatag cgaaacggaa
    54061 gatgacgtag taaacgacaa accagattgc cgccaccact ggcaccatgt accacttggt
    54121 tgaaagaccg tgcaaaatac cgaacaccac gaagtcgatg atattgccgt cggtattacc
    54181 gatggtgacg ccgagtacag acatgacggt gaagccgagg ccggttaaca gcgcgtggat
    54241 gacatacaga actggcgcta cgaacaggaa caggaattcc agcggttcgg tcgtgccgcc
    54301 aacgacacag gcgatcaggc cagaaatcag cagaccttta attttatggc gattttccgg
    54361 gcgcgcgcag tgatacatag ctaacgctgc acctggcaga ccgccgagaa acgcaggcat
    54421 tttgccttgc gaaaggaaac gcgtggcgct ttcagaaaaa ccgtgagtgg tcgagcaact
    54481 caattgcgcc tggaagatgg tcagtgcacc gctgacggtt tgaccgcaga cttcctgcgt
    54541 gccgcctgcg tcggtaaagc gaattaatgc caccagaatg tgatgcagac caaacggcaa
    54601 cagcagacgt tcaccggtac caaacagcat cggtccgaaa tcacccgcgc tattgatcat
    54661 atggcctaag ccgctaatac ccatggcgaa aatcggccag actaatggaa tcaccaggcc
    54721 gacaaggccc atcaccagcg aggagataat tggtacgaag cgcgtaccgc cgaagaatgc
    54781 cagcgcatcc ggcaggcgga tattatggaa tcgctcatgc agcatccaga cgataatacc
    54841 ggcgatcacc gcaccgagga tcccggtgtc gatcgactgg atcccaagaa tgctctggat
    54901 gttattggct ttcagaaccg cggcatccgt ggttggcaga atgcctttat tggtcaacca
    54961 gaagtttacc gcgaggttca ttaccgcata accgacgaag ccagcgaatg ccgctacgcc
    55021 tttgttttcg cgtgccaggc ccagcgggat ggcaatacag aacatgactg gcaggaaact
    55081 aaaagcaaac gagccaatct tactcatcca ggtaaagata gcctgcaaca agggattgcc
    55141 caggaccggg atcagggtga tgacatcgtg gctactaagg gaactaccaa tgccgagcat
    55201 aatgccgcag aacgacaata atgccacggg taacatgaag gttttgccta actgctggaa
    55261 gaactcccac agtgtgactt tcggtgctgt tttcgccgtc ataaaacgac tcctcgtaga
    55321 gaacaatctg gattcagtaa attgcgcgag aagataaaac gttttatcaa attttagtga
    55381 ggcataaatc acattacgca acgataatag cgggtataag ataaataaaa ggtaaaacgt
    55441 tttatctgtc acataatcag ggagtaggtc atctgcatgg ctaccgccaa aaaaataacc
    55501 attcatgatg ttgcgctggc tgcgggcgtg tcggtaagta ccgtttcgct ggtgcttagt
    55561 ggcaaagggc gaatctctac cgccacagga gaacgcgtta acgccgccat tgaagagctg
    55621 ggatttgtgc gcaatcgcca ggcgtcggcg ctgcgcggcg ggcaaagcgg cgtcattggt
    55681 ttgatcgtcc gtgatttatc tgcgccgttt tacgccgaat tgacggccgg attgacggaa
    55741 gctctggaag cgcagggacg gatggttttt ttgcttcacg gcggtaaaga cggcgagcag
    55801 ctggcacagc ggttttcact gttactgaat cagggtgtcg atggtgtggt aattgccggg
    55861 gctgcaggaa gcagcgatga cctgcgacgg atggcagaag aaaaagctat cccggtgatt
    55921 ttcgcttccc gtgccagtta tcttgatgat gttgatacgg ttcgcccgga caacatgcag
    55981 gctgcacagt tgttgacgga gcatctcatt cgcaatgggc atcagcggat cgcctggctg
    56041 ggagggcaaa gttcctcatt aacccgtgca gaacgggtgg ggggctattg tgcaactcta
    56101 ctaaaatttg gcctgccgtt tcacagcgat tgggtgttgg agtgcacttc cagccagaag
    56161 caagccgcgg aagctatcac ggcgctttta cgtcataacc cgaccatcag tgccgtggtt
    56221 tgctataacg aaactattgc gatgggggca tggtttggtt tgctgaaagc agggcggcaa
    56281 agcggggaaa gcggagtcga tcgttacttt gagcaacagg tttcgctggc ggcatttacc
    56341 gatgcgacac caaccacact tgatgatata cccgttacct gggccagcac gcctgcgcgg
    56401 gaacttggta ccacacttgc ggatcgcatg atgcaaaaaa tcacccatga agagacgcat
    56461 tcacgcaatc ttattattcc cgcccggctc attgcggcga aataatcctc tctttatctg
    56521 ctatacctgg tagtgtcctt tcctcaaggt taatgataaa aaatgtggca cacaggcggt
    56581 gccttttatt tttactgaag ttcacaggag gtttatgtgt ttaattctga caacctgaga
    56641 ctcgacggaa aatgcgccat catcacaggt gcgggtgcag gtattggtaa agaaatcgcc
    56701 attacattcg cgacagctgg cgcatctgtg gtggtcagtg atattaacgc cgacgcagct
    56761 aaccatgttg tagacgaaat tcaacaactg ggtggtcagg catttgcctg ccgttgtgat
    56821 attacttccg aacaggaact ctctgcactg gcagactttg ctatcagtaa gctgggtaaa
    56881 gttgatattc tggttaacaa cgccggtggc ggtggaccta aaccgtttga tatgccaatg
    56941 gcggattttc gccgtgctta tgaactgaat gtgttttctt ttttccatct gtcacaactt
    57001 gttgcgccag aaatggaaaa aaatggcggt ggcgttattc tgaccatcac ttctatggcg
    57061 gcagaaaata aaaatataaa catgacttcc tatgcatcat ctaaagctgc ggccagtcat
    57121 ctggtcagaa atatggcatt tgacctgggt gaaaaaaata ttcgggtgaa tggcattgcg
    57181 ccgggggcaa tattaaccga tgccctgaaa tccgttatta caccagaaat tgaacaaaaa
    57241 atgttacagc acacgccgat cagacgtctg ggccaaccgc aagatattgc taacgcagcg
    57301 ctgttccttt gctcgcctgc agccagctgg gtaagcggac aaattctcac cgtctccggt
    57361 ggtggggtac aggagctcaa ttaatacact aacgaaccgg taaacagccg tatgtgttgt
    57421 ttaccgggat agcgtgtgca attcatcaac gtctctgcta aacaactggc agccaaatca
    57481 cggctattgg ttaaccaata gctggatgaa aagcatacga atagagtgtg ccttcgcact
    57541 attcaacagc aatgataggc gctcacctga caacgcggta aactagttat tcacgctaac
    57601 tataatggtt taatgatgga taacatgcag actgaagcac aaccgacacg gacccggatc
    57661 ctcaatgctg ccagagagat tttttcagaa aatggatttc acagtgcctc gatgaaagcc
    57721 atctgtaaat cctgcgccat tagtcccggg acgctctatc accatttcat ctccaaagaa
    57781 gccttgattc aggcgattat cttacaggac caggagaggg cgctggcccg tttccgggaa
    57841 ccgattgaag ggattcattt tgttgactat atggtcgagt ctattgtctc tctcacccat
    57901 gaagcctttg ggcaacgggc gctggtggtc gaaattatgg cggaagggat gcgtaaccca
    57961 caggtcgccg ccatgcttaa aaataagcat atgacgatca ctgaatttgt tgctcagcgg
    58021 atgcgtgatg cccagcaaaa aggcgagata agcccagaca tcaacacggc aatgacttca
    58081 cgtttactgc tggatctgac ctacggtgta ctggccgata tcgaagcgga agacctggcg
    58141 cgtgaagtgt cgtttgctca gggattacgc gcgatgattg gcggtatctt aaccgcatcc
    58201 tgattctctc tctttccgcg ggctggtgac aactgagccc gcgtttcata tcgtaatttc
    58261 tctgtgcaaa aattatcctt ctcgggttcg gggaattcct ccctaaaata ttcaccgtag
    58321 ccatatgtca tgagagttta tcgttcccaa tacgctcgaa cgaacgttcg gttgcttatt
    58381 ttatggcttc tgtcaacgcc gttttaaaga ttaatgcgat ctatatcacg ctgtgggtat
    58441 tgcagttttt ggttttttga tcgcggtgtc agttcttttt atttccattt ctcttccatg
    58501 ggtttctcac agataactgt gtgcaacaca gaattggtta actaatcaga ttaaaggttg
    58561 accagtattt ttatcttaat gaggagtccc ttatgttacg tcctgtagaa accccaactc
    58621 gtgaaatcaa aaaactcgac ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa
    58681 ttgatcagcg ttggtgggaa agcgcgttac aagaaagccg ggcaattgct gtgccaggca
    58741 gttttaacga tcagttcgcc gatgcagata ttcgtaatta tgtgggcaac gtctggtatc
    58801 agcgcgaagt ctttataccg aaaggttggg caggccagcg tatcgtgctg cgtttcgatg
    58861 cggtcactca ttacggcaaa gtgtgggtca ataatcagga agtgatggag catcagggcg
    58921 gctatacgcc atttgaagcc gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta
    58981 tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga atggtgatta
    59041 ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac tacgccggga
    59101 tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat atcaccgtgg
    59161 tgacgcatgt cgcgcaagcc tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg
    59221 gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca
    59281 ccagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa ggttatctct
    59341 atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg ctgcgcgtcg
    59401 gcatccggtc agtggcagtg aagggcgaac agttcctgat caaccacaaa ccgttctact
    59461 ttactggctt tggccgtcat gaagatgcgg atttgcgcgg caaaggattc gataacgtgc
    59521 tgatggtgca cgatcacgca ttaatggact ggattggggc caactcctac cgtacctcgc
    59581 attaccctta tgccgaagag atgctcgact gggcagatga acatggcatc gtggtgattg
    59641 atgaaactgc agctgtcggc tttaacctct ctttaggcat tggtttcgaa gcgggcaaca
    59701 agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcag gcgcacttac
    59761 aggcgattaa agagctgatt gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta
    59821 ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg ccactggcgg
    59881 aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg
    59941 acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac cgttattacg
    60001 gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa aaagaacttc
    60061 tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggt gtggatacgt
    60121 tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag tgtgcatggc
    60181 tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga
    60241 atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac aagaagggca
    60301 tcttcacccg cgaccgcaaa ccgaagtcgg cggcttttct gcttcaaaaa cgctggactg
    60361 gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatcaac aactctcctg
    60421 gcgcaccatc gtcggctaca gcctcggtga cgtcgccaat aacttcgcct tcgcaatggg
    60481 ggcgctcttc ctgttgagtt actacaccga cgtcgctggc gtcggtgccg ctgcggcagg
    60541 caccatgctg ttactggtgc gggtattcga tgccttcgcc gacgtctttg ccggacgagt
    60601 ggtggacagt gtgaataccc gctggggaaa attccgcccg tttttactct tcggtactgc
    60661 gccgttaatg atcttcagcg tgctggtatt ctgggtaccg accgactgga gccatagcag
    60721 caaagtagtg tatgcatatt tgacctacat gggcctcggg ctttgctaca gcctggtgaa
    60781 tattccttat ggttcacttg ctaccgcgat gacccaacaa ccacaatccc gcgcccgtct
    60841 gggcgcggct cgtgggattg ccgcttcatt gacctttgtc tgcctggcat ttctgatagg
    60901 gccgagcatt aagaactcca gcccggaaga gatggtgtcg gtatatcatt tctggacaat
    60961 tgtgctggcg atagccggaa tggtgcttta cttcatctgc ttcaaatcga cgcgtgagaa
    61021 tgtggtacgt atcgtggcgc agccgtcatt gaagatcagt ctgcaaaccc tgaaacggaa
    61081 tcgtccgctg tttatgttgt gcatcggtgc gctgtgtgtg ctgatctcga ccttcgcggt
    61141 cagcgcctcg tcgttgttct acgtgcgcta tgtattaaat gataccgggc tgttcactgt
    61201 gctggtactg gtgcaaaacc tggttggtac tgtggcatcg gcaccgttgg tgccggggat
    61261 ggtcgcgagg atcggtaaaa agaatacctt cctgattggc gctttgctgg gaacctgcgg
    61321 ttatctgctg ttcttctggg tttccgtctg gtcgctgccg gtggcactgg ttgcgttagc
    61381 cattgcctca attggtcagg gcgttaccat gaccgtgatg tgggcgctgg aagctgatac
    61441 cgtagaatac ggtgaatacc tgaccggcgt gcgaattgaa gggctcacct attcactatt
    61501 ctcatttacc cgtaaatgcg gtcaggcaat cggtggttca attcctgcct ttattttggg
    61561 attaagcgga tatatcgcca atcaggtgca aacgccggaa gtaattatgg gcatccgcac
    61621 atcaattgcc ttagtacctt gcggatttat gctactggca ttcgttatta tctggtttta
    61681 tccgctcacg gataaaaaat tcaaagaaat cgtggttgaa attgataatc gtaaaaaagt
    61741 gcagcagcaa ttaatcagcg atatcactaa ttaatattca ataaaaataa tcagaacatc
    61801 aaaggtgcaa ctatgagaaa aatagtggcc atggccgtta tttgcctgac ggctgcctct
    61861 ggccttacct ctgcttacgc ggcgcaactg gctgacgatg aagcgggact acgcatcaga
    61921 ctgaaaaacg aattgcgcag ggctgataag cccagtgctg gcgcgggaag agatatttac
    61981 gcatgggtac agggaggatt gctcgatttc aatagtggtt attattccaa tattattggc
    62041 gttgaaggcg gagcgtatta tgtttataaa ttaggtgctc gtgctgatat gagtacccgg
    62101 tggtatcttg atggtgataa aagttttggc tttgccctcg gggcagtaaa aataaaaccc
    62161 agtgaaaata gcctgcttaa attaggtcgc ttcgggacgg attatagtta tggtagctta
    62221 ccttatcgta ttccgttaat ggctggcagt tcgcaacgta cattaccgac agtttctgaa
    62281 ggagcattag gttattgggc tttaacgcca aatattgatc tgtggggaat gtggcgttca
    62341 cgagtatttt tatggactga ttcaacaacc ggtattcgtg atgaaggggt gtataacagc
    62401 cagacgggaa aatacgataa acatcgcgca cgttcttttt tagccgccag ttggcatgat
    62461 gataccagtc gctattctct gggggcatcg gtacagaaag atgtttccaa tcagatacaa
    62521 agtattctcg agaaaagcat accgctcgac ccgaattata cgttgaaagg ggagttgctc
    62581 ggcttttacg cgcagcttga aggtttaagt cgtaatacca gccagcccaa tgaaacggcg
    62641 ttggttagtg gacaattgac ctggaatgcg ccgtggggaa gcgtatttgg cagtggtggt
    62701 tatttgcgcc atgcaatgaa tggtgccgtg gtggataccg acattggcta tcccttttca
    62761 ttaagtcttg atcgtaaccg tgaaggaatg cagtcctggc aattgggcgt caactatcgt
    62821 ttaacgccgc aatttacact gacatttgca ccgattgtga ctcgcggcta tgaatccagt
    62881 aaacgagatg tgcggattga aggcacgggt atcttaggtg gtatgaacta tcgggtcagc
    62941 gaagggccgt tacaagggat gaatttcttt cttgctgccg ataaagggcg ggaaaagcgc
    63001 gatggcagta cgctgggcga tcgcctgaat tactgggatg tgaaaatgag tattcagtat
    63061 gattttatgc tgaagtaaga aataacgccg gagagaaaaa cctccggcgt tttggctgtg
    63121 aattactgct gcggaatagc gggtacgacc ggaacgttta atgccggcat tgcaaacata
    63181 ccaacgaaat cttccagtgg cattttctgc ccatttaacg ttatctgacc gttagcatat
    63241 tgcaggctgg tggtgatggt attgtcctgc aaggtggtca gacggaacat ctgccccatt
    63301 gctgatgcac cttcaacttg ctgtttcgcc agttttttcg cttgatcttc ctgataacct
    63361 tccagcttcg ctacctgagt cattaactca gttgccatat ccaccggaat ggtcagtttc
    63421 gcatccagag atttaaccga acgatctact tcctgcgcca gcgtttgcgg cgcttcttta
    63481 gtcgttgccg gatctttcag gaacagcgac agattcagtg cactttcacc ctgactgttt
    63541 ttccagctta gtggcgcgat agtaatcacc ggatcgcctt tcaacatcag cggcagggcg
    63601 ctaaagaagg cttccgtcac tttctcctga taaagttcgg gattgttggc aatttctggc
    63661 tgtgccagca gcgcctgagt ttgcgcgtta tattgctggc taaactgatg ccaggcttcg
    63721 ccatcaattt ggccgacttt taaagtcagc ttgccgctgc ccagatcctg attctgtacc
    63781 ttcaggctgt ttagcgagta atccagttgg ctattgatcg ttttaccgtc attgaccaga
    63841 tccgatttac cgctgatctc catgccttcc agcagtgcca gttctttgcc ttccactgaa
    63901 atggtcattt tttccagtga cagtttttgg tttcctacgc gctcaccaaa acttgccagc
    63961 gtgctggaac cgtcggtttt cagattatta aaggtcaact gcactttctg gttgtattcg
    64021 ttaactgcgt ctatccgacc actttgcgcc tccccggaaa gggagatagc tttgccgtct
    64081 ctgtcagcat ttaactggaa ctcgccgccg ctaaaggcga ctttttcatc cttttgctcg
    64141 taattcagtg gcttgagcga aatatcggaa ctggaatcac cgctgtaacc aatgcgcgag
    64201 ttaatctcaa aaggcgtttc accttttgcc atatcaaaca gtggtttgct tacttcgtta
    64261 ttaaccagcg tggtttgaat tgatgccatc gacgggatca ggttcagttt tttaagctgg
    64321 gcaagcggga agggaccatg atcaaccgat tcgttgaaga tgacgctctg accgctttta
    64381 atccacggat tttctttccc ggcaatgggt ttcaccaaca gttgcaactg gctgctgaat
    64441 acgccgcgat gatagttttg ataactcact tccaggttgg attcaggagc ggtcagtttg
    64501 agttgcgcgt tcgcctgcgc gaccatctct tcgagatggg tttcaatctt cttgcctgta
    64561 taccatgcgc cgcctgtcca gactacgcct agcgcaacaa tgacgcctac cgctaccagc
    64621 gatttattca taatgattat ccataaaatg aaatcaggcg gactggccgc ctgaaggtgt
    64681 tataagcctt taataagctt agcaagagat gttaattttt tcagtaagct cttacagctt
    64741 gttgtaaaca cgcgctaaac ggccgtggcc tttgacagtc accggtgatt cgttggcggc
    64801 aataaacgct gattcacccg gtttaagctg taactgctga gaacctttac acagcgttgc
    64861 atcgccttcg acacagaaca aaatggcggc actctgctgg ctaatggtgg tttctttatc
    64921 actaaggtca tgcagcgaga aggcaaaatc atcaacagga atcgggaagt ccagttctgc
    64981 accttgtttc accggctggg tcaacaactg gttagccggt ttggcttcga acttcacatt
    65041 ggcaaccagt tccggaatat caatgtactt aggcgtcaga cccgcacgca gcacgttatc
    65101 ggagtttgcc atcacttcca gcgccacgcc ttgcaggtaa gcgtgcggtg tttcagcgaa
    65161 caggaacatc gcttcgccag gattcaattt caccacattc agcaacagcg gagagaacag
    65221 accgctgtct tccggataaa attcagaaat taaacgaatc gtttgccacg gttcaccctg
    65281 ctggctatcg agggccgatt ttaaaatcgc cagcgcgcgg gatttttctt caccctgcat
    65341 attcaacagg ctggcgaaca gttcgcttaa acgttcggca ttcggctgtt gtaaaaagtg
    65401 agcaatcgcc ggatgtgcac ctgcgaccgg ctggagtagg gagacaatct cggaaaattc
    65461 acgaaacgcg ttcatcgcaa ggaaaggcgt cagcgcaaaa accagctccg gcttgtggtt
    65521 aggatcttta tagttacgct cggcggcatc catcgggata cctgcggcat tttctttggc
    65581 aaaaccgatt tcagaattgt gtttgtttgg atgaacctga atggagagtg gctgtgctgc
    65641 gcataatact ttgaacagga aaggcagttc gccaaagcgt ttggcaacgg cctctccgag
    65701 cagagtcgat ttatcactct caatcacatc acgcagtgaa acgatatctc cggcggcatt
    65761 ctgcactcgt gaactgcttt tcggatgtgc gcccatccac agctcggcca tcggctggct
    65821 ggacggattt tccataccat aaagttcagt caacgccgtt ttgctgcccc aggcatagtt
    65881 ttgcactgag ttaatgagtt tttgcatgat caatccctgt tttaatgtgg aaattaatcc
    65941 cgctattaaa gcaagaatcc tacgggaagt aacctggagc cgcaaaaagt cgtactagtc
    66001 tcagtttttg ttaaaaaagt gtgtaggata ttgttactcg cttttaacag ggcaacggaa
    66061 cacccgccca gagcataacc aaaccaggca gtaagtgaga gaacaatgtc aaacaaaccc
    66121 tttcattatc aggctccttt tccactcaaa aaagatgata ctgagtatta cctgctaacc
    66181 agcgaacacg ttagcgtatc tgaatttgaa gggcaggaga ttttgaaagt cgcacccgaa
    66241 gcgttaactc tgttggcgcg ccaggcgttt catgatgcgt cgttcatgct gcgtccggcg
    66301 caccaacaac aggtggccga cattctgcgt gacccggagg ccagcgaaaa tgataaatat
    66361 gtggcgctgc aattcctgcg taactccgac atcgcggcga aaggcgttct gccaacctgt
    66421 caggataccg gcaccgcgat tattgttggt aaaaaagggc agcgtgtatg gaccggtggt
    66481 ggtgatgaag cggcgctggc gcgcggtgtc tataacactt atatcgaaga taatctgcgc
    66541 tactcgcaaa acgcgccgct ggatatgtat aaagaagtga ataccggcac caatctgcca
    66601 gcgcagatcg atctttatgc cgttgatggc gacgaataca aattcctctg tatcgccaaa
    66661 ggtggtggtt cggcaaacaa gacgtatctc tatcaggaaa ccaaagcgtt actgacaccg
    66721 gggaaactga aaaattacct ggttgagaag atgcgcacgc tgggtacggc ggcctgtcct
    66781 ccgtatcata ttgcgttcgt tattggtgga acttctgcag aaacgaacct taaaacggtg
    66841 aaactggctt ccgcgaaata ctatgatgaa ctgccaacgg aagggaatga gcacggtcag
    66901 gcgttccgcg atgtggaact ggaaaaagaa ttgctgatcg aagcgcaaaa tcttggtctg
    66961 ggtgcgcagt ttggtggtaa atacttcgct cacgacatcc gcgtgattcg cctgccacgt
    67021 cacggcgcat cctgcccggt cggtatgggc gtctcatgct ctgctgaccg taatatcaaa
    67081 gcgaagatca accgtcaggg gatctggatc gaaaaactgg aacataatcc aggcaaatat
    67141 atcccggaag agctgcgcaa agcgggagaa ggcgaagcgg tgcgcgttga ccttaaccgt
    67201 ccgatgaaag agatcctcgc acagttgtcg cagtatcccg tttctacacg cttatcgctt
    67261 aacggcacga ttatcgttgg tcgtgatatt gctcacgcca aactgaaaga gcggatggat
    67321 aacggtgaag ggctgccgca gtacattaaa gatcatccga tttactacgc gggtccggcc
    67381 aaaacgccgg aaggttatgc ctccggttct cttggcccaa cgaccgccgg acggatggat
    67441 tcttatgtcg atcaactgca agcgcagggc ggaagtatga tcatgctggc gaaaggcaac
    67501 cgcagccagc aggtgacgga tgcctgtaaa aaacacggcg gcttctacct tggcagtatc
    67561 ggtggtccgg ccgctgtatt ggcgcaggga agtattaaga gcctggaatg tgttgaatat
    67621 ccggaactgg gaatggaagc catctggaaa attgaagtgg aagatttccc ggcgtttatc
    67681 cttgtggatg ataaaggaaa tgacttcttc cagcagatac aactcacaca atgcacccgc
    67741 tgtgtgaaat aaacagagcc gcccttcggg gcggtttttt tatatggcac gaaagaccaa
    67801 acatttgtta tcaaatggta aataatgagt gagctaaaag ttgcttaacg aaagcaaaac
    67861 agaaagaaaa aattaatcag gtgaggagca ggccatgaat acagttcgca gcgaaaaaga
    67921 ttcgatgggg gcgattgatg tcccgggaga taagctgtgg ggcgcacaaa ctcaacgctc
    67981 gctggagcat ttccgcattt cgacggagaa aatgcccacc tcactgattc atgcgctggc
    68041 gctaaccaag cgcgcagcgg caaaagttaa cgaagattta ggcttgttgt ccgaagagaa
    68101 agcgagcgcc attcgtcagg ctgcggatga agtgctggcg gggcagcatg acgacgaatt
    68161 cccgctggct atctggcaga ccggctccgg cacgcaaagt aacatgaaca tgaacgaagt
    68221 gctggctaac cgggccagtg aattacttgg cggcgtgcgc gggatggaac gtaaagttca
    68281 ccctaacgac gacgtgaaca aaagccaaag ttccaacgat gtctttccga cggcgatgca
    68341 cgttgcggcg ctgctggcgc tgcgcaagca actcattccg cagcttaaaa ccctgacaca
    68401 gacgctgagt gaaaaatcgc gtgcatttgc cgatatcgtc aaaattggtc gtactcactt
    68461 gcaggacgcc acgccgctaa cactggggca ggagatttcc ggctgggtag cgatgctcga
    68521 gcataatctc aaacatatcg aatacagcct gcctcacgta gcggaactgg ctcttggcgg
    68581 tacagcggtg ggtactggac taaataccca tccggagtat gcgcgtcgcg tagcagatga
    68641 actggcagtc attacctgtg caccgtttgt taccgcgccg aacaaatttg aagcgctggc
    68701 gacctgtgat gccctggttc aggcgcacgg cgcgttgaaa gggttggctg cgtcactgat
    68761 gaaaatcgcc aatgatgtcc gctggctggc ctctggcccg cgctgcggaa ttggtgaaat
    68821 ctcaatcccg gaaaatgagc cgggcagctc aatcatgccg gggaaagtga acccaacaca
    68881 gtgtgaggca ttaaccatgc tctgctgtca ggtgatgggg aacgacgtgg cgatcaacat
    68941 ggggggcgct tccggtaact ttgaactgaa cgtcttccgt ccaatggtga tccacaattt
    69001 cctgcaatcg gtgcgcttgc tggcagatgg catggaaagt tttaacaaac actgcgcagt
    69061 gggtattgaa ccgaatcgtg agcgaatcaa tcaattactc aatgaatcgc tgatgctggt
    69121 gactgcgctt aacacccaca ttggttatga caaagccgcc gagatcgcca aaaaagcgca
    69181 taaagaaggg ctgaccttaa aagctgcggc ccttgcgctg gggtatctta gcgaagccga
    69241 gtttgacagc tgggtacggc cagaacagat ggtcggcagt atgaaagccg ggcgttaatc
    69301 tgcaacatac aggtgcagcc gtggaatgat caaacgaagc ggctgcgcct gaggtttata
    69361 acggtgctgc acattgtcgg catcgtaatt taacaactca ccaacgtccg gcacgcccgc
    69421 accattatcc cgattaatca gtgcaatcag cggtgtaggg caggcgtgtt gcacttgttt
    69481 ttgatctcct ttgtaccaga cgcgggcaat cggctgcacc ttcaccggac gtttgatttt
    69541 taacttcgcg ttctgtggca gggcagcgat atcctgatac tctcgctcca gttttctttg
    69601 ccactcctcg cgcgtccacg gtgcgacact gcgtggtgat ttcaggcttt tttccagctg
    69661 tgccaggact tcatcacgat gcaaattttt aatgatatgt ttattagccc aaccaaagcg
    69721 taaagtggcg gggtcgtaca gaacggtgag cgtgcggtaa gcattaaggg tgatcagccc
    69781 cggcaaatga cgatgcaccc attcaaaacg tgccgcggtg gggagttctg actcaaccgt
    69841 gacgatatgc tcgaacgtgg tcttgagttt attgatgtgc tgaatatgac tgaccaacgc
    69901 tgcttgcgaa aggttatcga cctggtaaca caacacgcca ggcagacgga cagcggcttt
    69961 gctgctgcga ttttcggact gttgttgaat aaataaatgg cggaaatgac gcaacgccag
    70021 cgactgcgcg tcgttgccga gatgttgttt cacctcaata cgattaagcg gattatgctc
    70081 atcctctttt tttacctccg gcaaagagaa cacgcgggca accaatagct tgtgttgctc
    70141 aagatgagcg gcaaatgcag ccagctcttg ttccatctgg cgaaaagtag tgttgagtcg
    70201 gtctacgaga tcgtaacgcg ccataatatt aaccacttta gttacaacat ccttaattta
    70261 tagcacagtg gtggtgactt gaccaccgtg cgcgtagtgt caggcagagg tcaattgcgg
    70321 gatgttatgc cataacggcc agctaaacga gaagcgggca ccacccagtt cgctggtgtc
    70381 acaattaacc gtaccgccca ttgccagtgc tatagagtgg acaattgcca gccccagccc
    70441 gcagccgccg gttgagcgat cccggctggg atcgaggcga acaaaaggtt caaagatatg
    70501 ttcgcggttt tctggggcaa tccctgggcc atcatcctca acaattaatg tcgctctatt
    70561 ccccgacagt agcaggctgg tttcaaccgt tgaatggcag tagcgcaggg cgttattgag
    70621 caaattatcc agcacgcgct ccattaagcg catatccaac gccgcataat ggccttgcat
    70681 gagcgttttt atccgtaccg ttttatcggg cgttactgcc tgaatatctg ccagatgcgt
    70741 tgatagccac aacggcaggt ctggttcgct aagatgaagc tcgttttgtg ggcgatcgag
    70801 tcgggcataa gtcagcagct cttcaattaa agcttcaagt tgactgatat cacgattcaa
    70861 cgcctgggat tcggcggcgc tcaggttatc gctcatctcc agtcgataac gcaggcgcac
    70921 taacggtgtt cgcagttcgt gagcgatacc gtcaataagc tgttttttgc tggcaatcaa
    70981 ggcgttgata ttgtccgcca tctggttaaa tgcaatacca agtcgttcaa agctcgaacc
    71041 ctcatcaaag tggatacgtt cattgagatg cccatcgcca aatcgttgcg ccgctgcttc
    71101 cagttttaac atatcctgcc agtgcggacg catccagata aacaccggaa aggcgaggga
    71161 aatagcaata aaagcgatca gggcgatatc cagcaatcgc atctgatgga ggtaataaag
    71221 ataaggaaca ggaccaactg ccagcacgta gtggctgcgc gggatacgct gcaaaaacgt
    71281 gtactgatcg tccagggcga caatttcgcc gccacgcagt cggtgcatgg aaatatcatc
    71341 aagatggtat ttactcagtg gctcgacacg cagatcgaaa gagagattta aatccatctc
    71401 tttcagcgtc ttaccccagt cgtgtggggg gatctcacgc aattcgctgc gcatcagata
    71461 cagcgaactg ttcatcaaat catccagcga ctgtttgccc gcgcgttcgg cggtaaattt
    71521 gtacaccagc ccaaccagca gagacatcac aaggaagcag acaaacaata acaggtaaaa
    71581 ctggataaac agttttttca tcgcttattc ccatgcatgg ggcgcaaaaa gatagccttt
    71641 gttacgcacc gttttaatgc gataaggttc agcggcgtta tcgagcagtt tttttcttaa
    71701 ccgcgaaata gccacgtcca cgctacgatc caggccgtca taactgacgc cgcgtaaatt
    71761 tttcagcaat gcatcgcggt ccatgatttg cccggcatgg gtagctaatt cccacaataa
    71821 ttcgaaatca gctgtcgaga gcgagatttc agtgttagcc agggtgacta cgcggttgat
    71881 aggatcgatg gtcaacgtgc cgaaatgcag ggctttgtag ggagtcagtg acgtttcctg
    71941 aagacctttg gtcagcgtgg cttgctcatt ctgacgcaaa tgcaaacgta aacgcgctaa
    72001 caaaacagca gggggcgtcg ttttgagaat atagtcgcag gcacccattt ccagtgccag
    72061 gatgtggttc atatcgctat cgagagaggt tagaagaaca atcggtccag accactttgc
    72121 gcgtaaatca cgacaaatgg tcatgccgtc cttgcctggt agcatgatgt cgagtaacac
    72181 caaatccgga ttttctcgca aaatggtttc ttcggcctgg tcgccgcgcg gctctacggt
    72241 aacctgcata tcatgttttg ccaggtacgc ggcaatcagt gaaccgactt ccgcatcatc
    72301 ttccacaaat acgatagtgt tcatattatt cacggtagat ataaaaaacg tcacaataca
    72361 ccgcgctgtt tttacttact atcaatcgtt tctaaacaag gtttcatcag gtattctact
    72421 gatgacttgt tatggaagtg ttaaggtaaa aagatggggc tggtaatcaa agccgcgctc
    72481 ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa cgaaaaatta ttatatcgcc
    72541 gggctgatcc cacttttccc gacctttgcg ctcatcgcgc attatattgt tgccagcgaa
    72601 cgcggcattg aagccttacg cgcgaccatc atttttagca tgtggtcgat tattccctat
    72661 tttgtttacc tggtgtcact gtggtatttc accggaatga tgcgcttgcc cgccgctttt
    72721 gttggttctg ttgcgtgttg ggggataagc gcatgggtgt tgattatatg ctggataaag
    72781 ctgcattaac gcagatgacg accgccatca agtgggaaac tgcgtccggt gacaaagcaa
    72841 ctggtaagta agtaatcgac caggtcgatc acttctttct cgccaggcgc ggttttcatc
    72901 agtgatttat tcagcgcctg ttgtcgatat tcggcatcat catgttcatt aaacaggatc
    72961 agcgatggcg caatagaatt cactttcact tccggtgcca gcttgcgggc aaacgagcgg
    73021 gtcatattat ccagtgccgc tttgcttgca gcatacgcaa tatgtttgtc gctaccgcgc
    73081 tccaccacat aatcggtaaa gtgaatgata tcgctggcgg cgtgtccgtg cccacgcagt
    73141 aatctttcca gcgcatggtt gagcaggtat ggggtattaa cgtggatctg catcatgcaa
    73201 gccagtacgt cggccagtgg cgcacccggt ttttccgcca tccacgcact ggcgttatgc
    73261 aaaatagcac gcagaccatg ggtgcttttt agtacttcat cggcaaacgc catcacaccg
    73321 tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg cattaatcag tccatcaatg
    73381 gctggatagt gtgtccgata gctgacaatc accggttgct tttgattaat gaaatgccat
    73441 gcgagggcga ggccgatgcg acgacctccg ccagtaatta atattggcaa gggctgggtt
    73501 ttacccatcg ttatctcctt tgctatccaa cgatgggatg aacgttatcc caccagcatc
    73561 caggtcgccg gaactgaggc aattaacagc atgccaatca gcaccatttc ctgacggttc
    73621 agtacattat catgtgtatg cgttttgcgc gcatacagga aaaccagcag ccccggtgca
    73681 tacagaacaa cggataacag caagtgcatt ggaccagagg cgtacaataa ccataagcca
    73741 taaatgcagg caccgacacc taccgcttta tgcagtggac gggttgcgat tttcagcaag
    73801 aacgcgccga ccaggaaata gggcaccaga atcatttctg aggcgatggt cagcaacgtg
    73861 ttgtaatcgg aaccggtcag ccagatgagt accagacaaa tttgcacgca gatgttggtc
    73921 agccacagtg aagcggatgg cgcagcttgg gcgttctggc gcgcaaaaat ccgtgggaat
    73981 gctttatgag tggcagccag gaacggaact tctgccgcca tgatggtcca gcttaagtac
    74041 gcgccgcaaa cggaaacgat caaaccggca gcgatgatga tttcgcccca tggtcccatc
    74101 atttccacca tcagaccggc cattgacggg ttacgaattt cagccagttc aggacgtgcc
    74161 accacaccca gcgaaagcag cgttaccagc aagtaaacgc ccagagcgga gagaaccgcc
    74221 agcagtgtcg ctttgccaac atcacgttta ttacgcgcac gcgcagaaac aaccacggca
    74281 ccttccacac caatgaaaac ccacagggtg atcagcatgg tgtttttcac ctgttcccaa
    74341 acaggtacgc caagtgcaag tccggtgaag tcgagcttga aggtatccag tttgaacatc
    74401 atcatcgcca gcacaacaaa cagacccagc ggcaacaatt ttgccagtgt cgccaccagg
    74461 ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa agtgaacaat ccacaataaa
    74521 gccgatgcac cgacgatcga ttgccaggta ttaccatcgc caaacaggcg caattccggc
    74581 gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca gataggagac gttggcgatg
    74641 actgcgcaca gccagtatcc ccatgcggaa caaaagccga ttagctcgcc aaacccttcg
    74701 cgggcatagg taaagatacc gccgtcaagt tcgggacgaa tgcgcgtgag gatcagcatg
    74761 gcaaaggcca gcaataaaat gccagcgcca gtaatacccc agccgatgag cagtgctgcc
    74821 gggctggcaa ctgccgccat attttgcggc agactgaaaa cacccgcgcc cagcattgag
    74881 cttaatacca gcgcggtgag tgcgctcagt cccagtttct tttccatcga tatccagtga
    74941 ttaaaacgtg ataagaagaa taaatattag ggcaaaacgc ataaaaatgg agtttgccac
    75001 tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa tggtcgcggg gaaatttgtg
    75061 actaaaaatg cgaaaggcgg catcaagccg ccttatagga gtaactgaat agttatttat
    75121 acagatctgc gctgacggtc aggttattac cacgttcctg ccactggcgg gtgatgtggt
    75181 aatacttagc acctttcttc gcggcacgtt tcgcaacctg ataggagact tcggtcatgt
    75241 tgccgtagtt gccagagaat ttgatgctgt cgaacgggac catcatcgct gctgtcgctt
    75301 tgttcagttc ttcaacttta gtgccatccg gaagcgtgac ggtgtaacgc ccgccttttg
    75361 atgactgggt ttcaaagaag cgaccgactt cagaacttgg tgatgcggta gtcgcaacac
    75421 ccgggatctc aactttcttc gcggcttcgc caccggcagc cagagctgca cgtcctgctt
    75481 cggaatctgc cgggatcaca tccgggctct ggacgatacg tttcttagca tcttttttat
    75541 agatgaatgc agtaatacgc tggttgccgc cctggttggc atcgatttga cgaacgatgt
    75601 agaaagagta ggcacctttc gcctttgccg ctttggtgat ggcatcattg acttctggct
    75661 ggctacgata gaagccctgg acggtgaccg tgtcaaacgg ttcaatcaga acagcctgat
    75721 ctttcggcag ttcgacaaca ccgttaatta cgcgattact tgtttcttct gctttttcag
    75781 catcggcttt atagaggtca gcgaccacac gccagttacc gctgttacca aaatcagaag
    75841 tgtcgacaac ataaaaagag gcggcacctt ctttatctgc gcgacgagaa acggctttca
    75901 ccgcttcgcc aatagcatta aaacgaccgg taaccactac acggtcaaaa ggtttaaccg
    75961 ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa
    76021 gcagtgccga cgccaggagg gtgttcttaa gcttcataaa aataatcctt cgccttgcgc
    76081 aaaccaggta ctggtattgt tattaacgag aaacgtggct gattattgca tttaaacggt
    76141 gtaactgtct gcgtcatttt tcatatcaca ttccttaagc caattttaat cctgctcaaa
    76201 tgaccgtcta tgcttaaaaa acagccgtat cagcatcatt actactgaag caactgaatt
    76261 gtataagtta atttaatgtt aagtagtgat tcgtgccggg gcgatgtctc gttttacccg
    76321 accgtcgaag acaattatca gtctttatcc ggcgttctaa ggtgtttatc ccactatcac
    76381 ggctgaatcg ttaatatttt gcgagttcac gccgaaatac tgatttttgg cgctagatca
    76441 caggcataat tttcagtacg ttatagggcg tttgttacta atttatttta acggagtaac
    76501 atttagctcg tacatgagcg gcttgtgtgg ctcctgacac aggcaaacca tcatcaataa
    76561 aaccgatgga agggaatatc atgcgaattg gcataccaag agaacggtta accaatgaaa
    76621 cccgtgttgc agcaacgcca aaaacagtgg aacagctgct gaaactgggt tttaccgtcg
    76681 cggtagagag cggcgcgggt caactggcaa gttttgacga taaagcgttt gtgcaagcgg
    76741 gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga gatcattctg aaggtcaatg
    76801 cgccgttaga tgatgaaatt gcgttactga atcctgggac aacgctggtg agttttatct
    76861 ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg
    76921 cgatggactc tgtgccgcgt atctcacgcg cacaatcgct ggacgcatta agctcgatgg
    76981 cgaacatcgc cggttatcgc gccattgttg aagcggcaca tgaatttggg cgcttcttta
    77041 ccgggcaaat cactgcggcc gggaaagtgc caccggcaaa agtgatggtg attggtgcgg
    77101 gtgttgcagg tctggccgcc attggcgcag caaacagtct cggcgcgatt gtgcgtgcat
    77161 tcgacacccg cccggaagtg aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc
    77221 tggattttaa agaggaagcg ggcagcggcg atggctatgc caaagtgatg tcggacgcgt
    77281 tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa agaggtcgat atcattgtca
    77341 ccaccgcgct tattccaggc aaaccagcgc cgaagctaat tacccgtgaa atggttgact
    77401 ccatgaaggc gggcagtgtg attgtcgacc tggcagccca aaacggtggc aactgtgaat
    77461 acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt caaagtgatt ggttataccg
    77521 atcttccggg ccgtctgccg acgcaatcct cacagcttta cggtactaac ctcgttaatc
    77581 tgctgaaact gttgtgtaaa gagaaagacg gcaacatcac tgttgatttt gatgatgtgg
    77641 tgattcgcgg cgtgaccgtg atccgtgcgg gcgaaattac ctggccggca ccgccgattc
    77701 aggtatcagc tcagccgcag gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa
    77761 aatgtgcctg ttcaccgtgg cgtaaatacg cgttgatggc gctggcaatc atccttttcg
    77821 gctggctggc aagcgttgcg ccgaaagagt ttcttgggca cttcaccgtt ttcgcgctgg
    77881 cctgcgttgt cggttattac gtggtgtgga acgtatcgca cgcgctgcat acaccgttga
    77941 tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg agcactgttg cagattggcc
    78001 agggcggctg ggttagcttc cttagtttta tcgcggtgct tatagccagc attaatattt
    78061 tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa
    78121 catatgtctg gaggattagt tacagctgca tacattgttg ccgcgatcct gtttatcttc
    78181 agtctggccg ggctttcgaa acatgaaacg tctcgccagg gtaacaactt cggtatcgcc
    78241 gggatggcga ttgcgctaat cgccaccatt tttggaccgg atacgggtaa cgttggctgg
    78301 atcttgctgg cgatggtcat tggtggggca attggtatcc gtctggcgaa gaaagttgaa
    78361 atgaccgaaa tgccagaact ggtggcgatc ctgcatagct tcgtaggtct ggcggcagtg
    78421 ctggttggct ttaacagcta tctgcatcat gacgcgggaa tggcaccgat tctggtcaat
    78481 attcacctga cggaagtgtt cctcggtatc ttcatcgggg cggtaacgtt cacgggttcg
    78541 gtggtggcgt tcggcaaact gtgtggcaag atttcgtcta aaccgttgat gctgccaaac
    78601 cgtcataaaa tgaacctggc ggctctggtc gtttccttcc tgctgctgat tgtatttgtg
    78661 cgcacggaca gcgtcggcct gcaagtgctg gcattgctga taatgaccgc cattgcgctg
    78721 gtatttggct ggcatttagt cgcctccatc ggtggtgcag atatgccggt ggtggtgtcg
    78781 atgctgaact cgtactccgg ctgggcggct gcggctgcgg gctttatgct cagcaacgac
    78841 ctgctgattg tgaccggtgc gctggtcggt tcctcggggg ctatcctttc ttacattatg
    78901 tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg gtggtttcgg caccgacggc
    78961 tcttctactg gcgatgatca ggaagtgggt gagcaccgcg aaatcaccgc agaagagaca
    79021 gcagaactgt tgaaaaactc ccattcagtg atcattactc cggggtacgg catggcagtc
    79081 gcgcaggcgc aatatcctgt cgctgaaatt accgagaaac tgcgcgctcg tggtatcaac
    79141 gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg gacatatgaa cgtattgctg
    79201 gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg acgagatcaa cgatgacttt
    79261 gctgataccg ataccgtact ggtgattggt gctaacgata cggttaaccc ggcggcgcag
    79321 gatgatccga agagtccgat tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac
    79381 gtgattgtct ttaaacgttc gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc
    79441 ttcaaggaaa acacccacat gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg
    79501 aaagctctgt aaccctgacg gcctctgcgg aggccgtcac tctttattga gatcgcttaa
    79561 cagaacggcg atgctttgac ctcccgctgt ttgttcaagc gcaattttga caataattgt
    79621 taacggcacg gaaagcagca tacccaccgg tcctaacaac catccccaaa aaatcaacga
    79681 caaaaatacc accaatgtgg aaagtcccag cccacgcccc atgatgcgtg gctccagaat
    79741 attgccgaag accagattaa tcagcagata tcccgccagc accaacaacg cttcgtagaa
    79801 gccattaaac accagtacct gagcgatagg ggggattgcc gcgagaactg aaccaatatt
    79861 agggatgtaa ttaagcgcaa aggccagcaa tccccagaca aaagcgaagc gaacatcgag
    79921 ggcggcgagc atcgcccatg cgaccaggcc ggtgatgatg ctgatggctg ttttcagcac
    79981 cagataatga gacacactgt caatcgcacg ctgaatcgcc gccatccctt caaccggacg
    80041 cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact tcgagcagca taaacagcac
    80101 cgtcagcagc aataaaaata ttgatgacat ggcattagat aactgcgtca ataagttggt
    80161 gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc agttggtcaa ctgagacgtc
    80221 aatccctacg cgttgcaaca acggctcaag agcttgcagc ggcgtcataa tagagttgcg
    80281 atattgcggt aacgtccgcg tcaactcgtt gagcgcggaa cccagataag ctaatagcaa
    80341 caccatcgcc atcacgatga tggtcatcaa aatcgacacc gccagtacac gcggcactcg
    80401 ccagcggacc atgtgttgca ccagcgggtt aagaataaca gcaataaata atgcgagaat
    80461 aaacggcacg atgatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc
    80521 caacataatg acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc
    80581 cgttttttct tcctttgcta ataataatgt ctttttcgct ccgtcatcat aacgaaaatc
    80641 aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat tcttaagcta tgatacaaat
    80701 caggcgtgtt caactaccga ggacaattat catccgcgat gacgagaagc aacactgcgg
    80761 ataattgtaa tattatggac aatttgttca ggacgttatt ttctacattt acccatcttc
    80821 gtacctcatc cactatcttg ctcgtaggtg agcagcactg gcgcaacgcg ctttagtcct
    80881 ttcttctgcc taagcttgcc ctatacgcgg ggcttgacca ctttactttg gtttcgctga
    80941 attaagcgaa aattaaaata attctcttgc aggagaagga caatgtatat ttattggatt
    81001 ttattaggtc tggctattgc tacagaaatt accggtacgc tgtctatgaa atgggcgagc
    81061 gtcagtgagg gaaatggcgg ctttatttta atgctggtga tgatttctct gtcgtatata
    81121 tttctctctt tcgctgttaa aaaaatagcc ttaggtgtgg cttatgcgct gtgggaaggt
    81181 atcggtattt tatttattac cttgtttagc gttttgttat tcgacgaaag tttatcgctg
    81241 atgaaaattg ccgggttaac caccctggtc gccgggattg tgttgatcaa atcaggtacc
    81301 cgtaaagcgc gtaaacctga actggaggtg aaccatggcg cagtttgaat gggttcacgc
    81361 cgcctggctg gcattggcaa tcgtgctgga aatcgttgct aacgtctttt tgaaattttc
    81421 tgacggcttt cgtcgcaaaa tatttggctt gctctccctg gcggcggtgc tggctgcctt
    81481 tagtgcgctt tctcaagccg ttaaagggat cgacttgtct gtcgcttatg cattgtgggg
    81541 cgggtttggt attgccgcca cgttagccgc aggttggatc ttgtttggtc aacggttaaa
    81601 tcgtaaaggc tggattggcc tggtcttgct gttggctgga atgatcatgg tgaaacttgc
    81661 ctgatgaaga cgctgcccgc gctgtcgggc agcgtttgaa cattattttt gcgacagttg
    81721 atccagcttg tcgcgaaaac cggtaacaga aatggcccgg ttatcggcgc gccagcgatc
    81781 tttcgcggca ggagccgaac tttgcacccc aattaattgc cagccgtcat cggtatgcaa
    81841 catcagaggc gaaccgctgt cacccggcaa ggtatcgcat tgatgtgaca tcaccgacgt
    81901 ttgcgcccag ccagtcactt cacagttttg atgactgtac aacgtatcga gatgatcttc
    81961 aggatatcct gcctgagtca ctttacgacc tgccgctttt aatgcggcgg taagcgcggc
    82021 tttatctccc tcaaataacg gcaacggcgt aatgccagaa gggggattac gtagcacaat
    82081 caatccgaag tcccacggcg cggctgcggg aggtacaatc caaccatccc catctgcttt
    82141 taaccgcttt cccagtgtcg gatcaacgcg gccttctatg tcgtggatgt catagcgcca
    82201 aagaccttta tttgacacaa aacgcagcgc cactgcttta tcggctttac cctttggagg
    82261 tgtcaataaa cagtgtcctg ccgttaatgc cagattgggt gcaatcagcg tcgccgtaca
    82321 taaattgccg ctggccgttt ccagttgccc aaccgcatcc cacggtgatt gggtcgtgtc
    82381 attcactggc acacgatcat catgaccaaa aaacagggtg ctgacctcat cgtttgccga
    82441 tttggcaacg tctggtttat ctgcaaacac aaaagcagac gtcaaactaa ttgcacccaa
    82501 cactacagca atggttgtac gcatatcaca ctctggtggg taattatgat tattaaaagc
    82561 aaaccctcat aaatactata gacgggacgg gatgaaagtg ggagtaaaat cagataacta
    82621 cagtcaggaa agataaaaac gggcggcaat cagtgcgcat aaaataagga tgataagaat
    82681 gaactcaaag cgataacggc ccaccatact accctccgct aaaggcggtg ctaaacgtta
    82741 cccgcttagc accgtaattt tcaggcgcga gggggcgcgc cagcattact gttgaaaact
    82801 tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat ggctgtgttt cccggcgtgt
    82861 ttcttcgctg cctgcgcttt ttgttcaggg gctttctgtt cagctttcgt atttttatga
    82921 tgctttttag ccgcctgcgc tttctgggca ggtgctgctt tatgctgttt tttatgatgt
    82981 gtagttttcg ccggcgctgc tttggtggtc gtcgcagtcg gagcaggtgt ggtcgcagtc
    83041 tctgcagcaa aggcggcaga agacagaccc atagcagcgg caacaaccag agctaatact
    83101 tttttcattg tcataccctc aatttgtttt ttcatttaac cccactgcgg ggccgttgaa
    83161 ataactatat ccctgggaat ggcagacttc cgtgagtggt tggtttcagc gtgtaacgat
    83221 atgtacaaac gctgaataaa ttacagcatt gataataacg tattgtgact taagggaaat
    83281 ttagctacca ataatagtag tcttgatcgg gttaactatt ttactactta caaagcggta
    83341 gaataactgc gcatcagtaa taaatgacac acagcaaaat gaatccgttt atttgggtac
    83401 ttataatcct gatgacgcta gacgcgctgc gggaattggc tggcgcttcg tctattttag
    83461 gatggctatt aacgttggtt tgagcaggca ataagtccgg acgggtattt accgcagtcc
    83521 ggacttattt ttcaggcgtg cagacgacga tgcaaacgcg tcccgaccag tagagcaatg
    83581 accagcatca gagcaataaa tgcgccgacg ccgttccagc catagttatg ccagaaaaca
    83641 ccacccagcg tcccggcaat actcgacccc agatagtaac tgaacagata cagcgaggag
    83701 gcctggcctt tagcgcgttt tgcacggggg ccgatccagc tgctggcaac tgagtgggct
    83761 gcgaagaatc ctgctgagaa gagtaacatt ccggcaaaga tcagccatag cgagctgaat
    83821 aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa acaacatcac tggaccacgc
    83881 ccatagcggg tggtcatggt tccggctttg ggtgagctcc atgtaccggt caaataagcc
    83941 agcgataata agccaaccac ggcctgactg acatgccagg gggagagcat caaccgatag
    84001 ccgatgtaat taaacagcgt gacgaacgac cccatcagca aaaagccttc tgcgaacaat
    84061 aacggtaatc cccggtcacg ccagtgcaga cgaaagttga taaacaacga cttaggtcgc
    84121 agcgaagtcg ggcgaaaatg gcgtgattcc gggaggattt tccagaacat caacgccgag
    84181 gctaacgcga aacaaccgat agccgccaga gcaattcgcc agttgaaaaa gtccgtgaag
    84241 acaccgctaa ttaagcgtcc gctcatgccg ccaattgagt tgccgctgat atacaacccc
    84301 atcgaaaagg ccacgaaact ggggtggatt tcctcgctaa gataagtcat gccaacagct
    84361 gccacgccac ttaacgaaag cccaatcaag gcgcgcataa tcaaaatgcc gtgccagctg
    84421 gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca gtagcgctgt gaccatcact
    84481 ggtttgcgac caatggcatc ggataggggg ccagtaaaca gcaaaccaat agccaacatc
    84541 gccgtggaaa tggacagtga aatactactg ttcgcgggcg ttaagccaaa ctcctgcgaa
    84601 agcaccggaa ggataggctg cacacaatag agaagtgcaa atgttgccag tccggcagag
    84661 aacagcgcca gggtgacgcg cataaattgc ggcgtaccgc gtttaataaa ttgatttggc
    84721 tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc catcaacagt tgtagtacgg
    84781 ctcacttgaa atccttgcta aatatgcctg tggatcaggc ttatacatag gttaggagaa
    84841 tcgaattgtt ctgtctaata tattaataat ctcaaataag atgttttaaa tatgaatatt
    84901 gaacttcgtc atctgcgtta ctttgttgct gttgcggaag agctgcattt cgggcgcgcc
    84961 gctgcccgcc tgaatatttc gcaaccgccg ctaagtcagc agattcaggc gctggagcaa
    85021 caaattggtg cccgactgct ggcacgaacc aatcgcagtg tattgctgac ggcagcagga
    85081 aaacagtttc ttgcagatag tcggcaaatc ctgtctatgg tggatgacgc tgccgctcgc
    85141 gctgaaagac tgcatcaggg tgaagcgggg gagttgcgca ttggttttac ttcgtcggct
    85201 ccttttattc gggcggtgtc cgatacgtta tcgctgtttc gccgagatta tcccgatgtc
    85261 catttacaaa cccgcgaaat gaacactcgc gagcaaatcg ctccgctcat tgaaggaacg
    85321 ctggatatgg gattgctgcg taacacagcg ttaccggaga cgcttgaaca cgcagtcatc
    85381 gtccatgaac cgcttatggc gatgatcccg cacgatcatc ccctggcaaa taacccgaat
    85441 gtaacgctgg ctgaactggc gaaagaaccc tttgtctttt ttgatccgca cgtcgggaca
    85501 gggctgtatg acgatattct cgggctgatg cgacgttacc atttgacgcc cgtcatcact
    85561 caggaggtgg gcgaggcaat gaccatcatc ggtctggttt ccgccggtct gggtgtttca
    85621 attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa tgcgctgggt gccgattgct
    85681 gaagaggatg cggtttctga aatgtggttg gtctggccga aacatcatga acaaagtcca
    85741 gctgcgcgta actttcgtat tcatctgctg aatgctctca ggtgagggaa atttcagcga
    85801 aaaagcccga aaaatgtgct gttaatcaca tgcctaagta aaaatttgac gacacgtatt
    85861 gaagtgcttc accatagcct acagattatt tcggagcgcg aaaatatagg gagtatgcgg
    85921 tggttgctga aaaccagcct gggcacattg atcaaataaa gcagaccaac gcgggcgcgg
    85981 tttatcgcct gattgatcag cttggtccag tctcgcgtat cgatctttcc cgtctggcgc
    86041 agctggctcc tgccagtatc acaaaaattg tccgtgagat gctcgaagca cacctggtgc
    86101 aagagctgga aatcaaagaa gcggggaacc gtggccgtcc ggcggtgggg ctggtggttg
    86161 aaactgaagc ctggcactat ctttctctgc gcattagtcg cggggagatt ttccttgctc
    86221 tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca ggaactggcg ttaaaagatg
    86281 actcgccatt gctggatcgt atcatttccc atatcgatca gttttttatc cgccaccaga
    86341 aaaaacttga gcgtctaact tcgattgcca taaccctgcc gggaattatt gatacggaaa
    86401 atggcattgt acatcgcatg ccgttctacg aggatgtaaa agagatgccg ctcggcgagg
    86461 ctctggagca gcataccggc gttccggttt atattcagca tgatatcagc gcatggacga
    86521 tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga tgtgattcag gtggttatcg
    86581 atcacaacgt gggggcgggc gtcattaccg atggtcatct gctacacgca ggcagcagta
    86641 gcctcgtgga aataggtcac acacaggtag acccatatgg taaacgctgt tattgcggga
    86701 accacggctg cctcgaaacc atcgccagtg tggacagtat tcttgagctg gcacagctgc
    86761 gtctcaatca atccatgagc tcgatgttac atgggcaacc gttaaccgtg gactcattgt
    86821 gccaggcggc attgcgcggc gatctgctgg caaaagacat cattaccggg gtgggcgcgc
    86881 atgtagggcg cattcttgcc atcatggtga atttatttaa cccacaaaaa atactgattg
    86941 gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt catctcagat agcatccgtc
    87001 agcaggccct tcctgcgtat agtcagcaca ttagcgttga gagtactcag ttttctaacc
    87061 agggcacgat ggcaggggct gcgctggtaa aagacgcgat gtataacggt tctttgttga
    87121 ttcgtctgtt gcagggttaa cattttttaa ctgttctacc aaaatttgcg ctatctcaat
    87181 ttgggccagg aaagcataac ttagactttc aaggttaatt attttcctgg tttatatttg
    87241 tgaagcataa cggtggagtt agtgatgctg aagcgtttct ttattaccgg tacagacact
    87301 tctgtaggga aaacggtggt ttcccgcgca ttgctacaag cgttagcctc ccagggaaaa
    87361 acggttgcgg gatataaacc cgtagcgaag gggagcaaag agacacccga agggctgcgt
    87421 aataaagatg ccctggtgtt gcagagtgtt tcaaccatcg aactgcctta tgaagcagtt
    87481 aatcctatcg cgttaagcga agaagaaagt agcgtggcgc acagttgccc aatcaattac
    87541 accctcattt caaacggcct ggcaaacctg accgaaaaag tcgatcatgt cgtggtagaa
    87601 gggactggcg gctggcgtag tctgatgaat gatttgcgtc cactctctga atgggtggtg
    87661 caggaacaac tgccggtgtt gatggttgtc ggtattcagg aaggttgcat taaccatgca
    87721 ctgctaacag ctcaggcgat tgccaacgac gggctgccgc tcattggctg ggtggctaac
    87781 cgaatcaacc caggactggc gcattatgcg gaaatcattg atgtgctcgg taaaaaactt
    87841 ccggcaccgc tcattggtga actgccttat ctgccgcgcg ccgaacagcg tgaactgggg
    87901 caatacatcc gcttagctat gctgcgcagt gtgctggcgg tagatagagt cacggtgtaa
    87961 cgtccgcgag attaccgacg caattacgca ggcaatcaat aaaccggaga gtagctgata
    88021 ctccccggtc atttcacata tcatcaacgt cgacataatc ggcgcgtgcg tggttgccgc
    88081 caacagtgtc gccatcccgg tcaatccgag taaaagcgta atttcttcgc catcagggaa
    88141 ccataatccc aggctacgac catacaacat gccaatggca agaccgataa atagcgtcgg
    88201 tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc agcacggcac acagtttaca
    88261 aaggaagatc ccggcaatga tcattaacag tggcggggcg gttaaaaagg attgtacggt
    88321 gctatagccg ttgccccaca ctgcaggtgt gaacagggaa agcagaccca cgatcaaccc
    88381 gcctagtgcc agttgccagg gcggcgcaag tttgagactc acaaatccac gatgacaggc
    88441 gttcattaac gttaacaaca gtggtccgca cagacctgcc agcacacctg tactgataat
    88501 cagcgcatag tcacgagcct gaaccgtcac tgagagttgt acgctgtaga gtaacgcgtc
    88561 gctatgatta atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc
    88621 gagagaggcc aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag
    88681 cggggcacga taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca
    88741 ctcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat
    88801 catcgcacct tcgcgaccaa ttgcactgcc gctggttact accagcagag aggcaagcga
    88861 tttaaccagg cttgctgcgt aatcaaactg accatcggtt tgcaacgctt ccatgtaatc
    88921 ggtcggcgca tgagggcgtt gctgggtaaa tttctgccag accatcaaca acaaacccgc
    88981 cgccagtccg ccgagcgccg gagttagcag ccgtcgccag gtggaaagat ttgtcgctgc
    89041 attgaccaga ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg
    89101 gcgaaacccg gcaacggcaa aggccgcgag aataccgacg actgttgcga taagcagacg
    89161 gcggaacata gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca
    89221 atgaaacacg ggcgactatt ttgcggcaaa agaggccagc tgcaaaagca gtgcgtgctt
    89281 taagagataa aaaaacgtga cactgtaacg tattattgcc gaattgcgcg taatcgtcac
    89341 catccggcaa tattacggtg atcctatcga aacagcggtt taaccgcgac aggaattaac
    89401 agttgtgatt gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa
    89461 gggtgttctg ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc
    89521 catgccagca attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc
    89581 aacagcagtg aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct
    89641 ttctcgcgca tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga
    89701 tccagccaga ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc
    89761 tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct
    89821 aatgacgctt ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc
    89881 agaggtgcgg cttccgcact ctctggagcg taataaaaca gcgcgcccaa cacgcgcgcc
    89941 gcgatagaaa aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg
    90001 ggttgccccg caccaatttg ttaacctgca atagccattc ctaccgtcat gtgcaggcca
    90061 taaaagagca cacgcccgat catctcaccg ccaagaatga ggattaaccc cagaataagt
    90121 ccggcaacgt gaggttcacg gcgacgtatg agcggacaaa gccagcatcc tagacctgcg
    90181 cataacaata ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct
    90241 tgttgcacgg atgagtgaat agacgacaga gacaaacctt gcagcacaat caccccggca
    90301 caagcgatca gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg
    90361 gctgcccgca gaattgcggc agcaagtatt ggaccactca acaaaaccgt caggaagaaa
    90421 gccagcgtag tgtaaccgtt atgccaggtt ggcacagtgt cgatttgata cacgcaggtc
    90481 atcatccaga cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac
    90541 gcttgcggca ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat
    90601 atggaaccgg cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta
    90661 aacgcccgca gtggtgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac
    90721 agccagagga aaaacatgcc acgaacaatg tgctgacggt ctgcgtcagt tttggcggcg
    90781 aaccagccaa ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag
    90841 ataaccagtg gccattcatg ccatccattt cccattttac acttcctccg gattagccag
    90901 ataaccagtg gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg
    90961 ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagca agagtgccgt gcttctggcg
    91021 cagctcttca attggaccga actcaagtgc acgcagcggg caggattcga cacatatggg
    91081 ttgtttcccc tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc
    91141 agcattgtac tgtggcgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca
    91201 aacatcttca tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca
    91261 gacttttgta catgcggggt catcgcaatg gttacaggag atggagagat aataagcgaa
    91321 cacgttctgg tgccagacgc cattatcttc ctgccagtcg ccgccagtat attcataaat
    91381 acggcggaaa ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt
    91441 tttacagcca gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta
    91501 ctccttaaac cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgccagcg
    91561 gtgaagggcg gtgagaggtc agaatattaa tactgccgcc gtgatcgacc cggtcaccaa
    91621 acatatccgc tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg
    91681 gagtcacttt tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc
    91741 catgacggat accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg
    91801 cctgctgtaa cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc
    91861 ccgttaactg cagtggatag gttttccgca ggggatcgtc ccagccatca aaacctgggg
    91921 tgtacgcagg aaggggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct
    91981 tcgccagtcg ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct
    92041 gtggatcttc acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa
    92101 agatcccggt cgttttcatc tcctcgtagt cgggcatctc aggggtacgt tccttcgttt
    92161 tcgcatggag atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa
    92221 cgtccggtcc taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa
    92281 attttgctga ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat
    92341 gagagataag gtcttcttgc tctgttggca tcaggtcggg caacaggata tcgcaatact
    92401 tagccgaggc cgtcatgaag tggtcaatgc cgacaatcat ctcgcacttg ctgtcatcct
    92461 gaagcacctc atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt
    92521 agcaccataa aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg
    92581 tcgcggtcat ttccgtacca tggtcgatgg catctgtcca tgtaaagacg gaaatctgcg
    92641 ttttaacagg attctcgagc atcgggaacc attctacccc cagatcccag ctaccttcgc
    92701 gtacgcctga gttgccgccg tttatgccga cgttaccggt gagaacggaa agcatggcaa
    92761 tagcgcggga tgtttgctcg ccgttggagt gtcgttgtgg cccccaaccc tgacaaatat
    92821 aagcaggttt tgctgaaccg atctctcgtg ccaactggat aattttttct gccgggatgc
    92881 tggtgatttt tgctgcccat tccggcgttt tagctatgcc gtcaggccct tcgcccagaa
    92941 tataggcttt ataatgcgcg ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac
    93001 caacacaata tttgtcgaga aatggctgat cgaccatgtt ttcagtaatc agtacccagg
    93061 caatcgcaca ggccagtgcg ccatcggtgc cagggcgaat gggcagccat tcatcttcac
    93121 gcccggcagc agtgtcgtta taacgtggat cgatgacgat catgcgtgcg tttgaacgtt
    93181 cgcgggcttg ctcgacgtag taagtgacac caccgccgct catccgcgtt tctgctgggt
    93241 tatttccgaa cataacgacc agtttcgtat tggcgatatc atccgggctg ttgccatcat
    93301 tggcaccgaa catataactc attgcggcac tgatctgtgc ggtactgtag ctaccatagc
    93361 gactgagaaa gccaccgcaa gagttcatca gacggtacgg gacgtttgag ttggtgatgt
    93421 ttccgccatc tacgcctgtt ccgtacagga catgtacagc ctcattgccg taatctttca
    93481 ggatccgccg aagattatca ctgatggtat ccagggcttc gtcccaactt atccgttcaa
    93541 atttaccttc accgcgcttg ccgacgcgct tcatgggata tttcaaccta tcaggatgat
    93601 tcatccgtcg gcggatagag cgcccgcgta aacacgctcg aacctgatga ttaccgtaga
    93661 cgtcgtcacc tgtcgtatca gactccaccc agtacacggt gtcatctttc acatgcaaac
    93721 gtaacagaca gcggctcccg cagttaacgg tgcaggaact ccacaccgct ttctcttcta
    93781 ccggagcctc tgccgcccgg accatttggg aaaacggcaa agtgaaagca ctgcttgcca
    93841 gcgcgagact gccaagtgcg gaggttttca tcagacttct acggctgatt tcagccttca
    93901 tgagcgcctc tgtggtatgg attttcatca ttactcactt attgcttttc aaacaaaatg
    93961 tcatgccaga atttatggtt gtagtgggtt atattttttc gatctcgacc agattcgtgt
    94021 gctgtgggtt tcccttcgcc agtggtgaag ggcgcagagt ggttagcgta ttcacacagc
    94081 ctccatggtc gattttatcg ccagacatat tggcctcgtg ccaggctccc tggcccatag
    94141 cgctaacccc agggagaata cgtggtgtca ctttggctgg tagccgaact tcgccacgat
    94201 ggttaaacac ccgcaccata tcgccgttgg caatcccacg tttctgtgca tctatagggt
    94261 tgatccacac ctcctgacgg caggcagctt tcaggacatc aatgttgccg taggtcgagt
    94321 gagtacggga tttgtaatgg aaaccaaaca gttgcagtgg gaaggtacta cgttcagggg
    94381 agtcccagcc ttcaaaggtt gaggcataaa ccggcagtgg gcttatcact tcatcttttt
    94441 ccagttccca ggtacgggca atttccgcca gcttgctgga ataaatttca atcttaccgg
    94501 aaggcgtttt aagtggattt gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat
    94561 gaccattggg atctttacgc ttatagatac ccattttttt cagttcgtcg taagacggta
    94621 acgccggatc tttggcaagc attttggcgt acagatgttg taaccattgt tcctgcgtgc
    94681 gaccttcagt gaacttttga tagacgtcag gtccaagacg tttcgcgact tcactcagga
    94741 tccagtaaat cggtttacgt tcgaattttt cgctggtgac tggctggagg aaaatgagat
    94801 atcccatgtt accggcgtag tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt
    94861 ctggcagcag aatgtcggca tattttgccg atgaggtcat aaagttttcg atgaccacaa
    94921 tcatttcgca tttcgattcg tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct
    94981 gattaacgag ggtatttccc gcgtagttcc agatgaactt aatgggcaca tccagtttat
    95041 ctttgccccg gacgccgtcg cgaattgccg tcatttgcgg accatgatcg atagcatccg
    95101 tccagctgaa gcaggagatt gacgttttga ccggattatc cagcaccggc aggcgttcta
    95161 tggtaatggt ataggtcgat tcacgcgcgc cactatttcc gccgctgatg ccgacattgc
    95221 ccgtcaaaat aggcaacata gcaatagcgc gtgcggtcag ctcgccgttt gcctggcgtt
    95281 gtggccccca gccctggcag atataagcgg gtttcgctgt gccaatttca cgagccagct
    95341 tgatgatacg gtccaccggg ataccggtaa tttgcgaagc ccactgcggc gttttcgctg
    95401 ttttatcatc accttcacca agaatatagg ctttatagtg accatttttt ggtgcatctg
    95461 cgggtaaggt tttttcgtca tagccgacgc agtatttatc gagaaaaggt tgatcaacga
    95521 gattttcgtt aatcaatacc caggcaatcc ccgcaaccag cgcggcatcg gtgcccgggc
    95581 gaataggaag ccattcatct tcacgaccag cagccgtatc ggtatatcgc ggatcgataa
    95641 caatcatttt ggcgttcgat ttctcgcgcg ctttttcaag aagataagtg atgccaccgc
    95701 cgctcatgcg ggtttctgcc gggttgttac caaacatcac gaccagcttg ctgttttcaa
    95761 tatccgtggt gctgttgcca tcattactgc cgtaggtgta gggcatggca caggaaattt
    95821 gcgcagtgct gtaggagcca tactggttga gtgaaccgcc gtagcagttc atcaggcgtt
    95881 tgaccgccga ggctgatggc gaagagcggg tcatattgcc gccaacaatc cccgaagagt
    95941 actgaatata tacagcctca ttgccatatt gttcgacgtt ttttttcagg ctactggcga
    96001 tagtatccag ggcttcatcc cagctaatcc gttcgaattt gccttcaccg cgtttaccca
    96061 cgcgtttcat tgggtaattc aagcgatcgg gatgattaat acgccggcgg atggagcgac
    96121 cgcgcaaaca ggcgcgtact tgatgattgc catactcgtc gttgccggtg ttgtcagttt
    96181 ccacccagat cacttcatta tctttaacat gcagacgaag tgcacagcgg ctaccacagt
    96241 tgacggaaca ggcaccccag accactttct cgctggcctg ttgtaccgct gccgctgcac
    96301 tgcgcagggt gaacggtaaa gaaaaaccgc ctgcagccag cgccagagaa cctatcgcgg
    96361 tagatttaac gagtgttctg cggctgatgc ccaccattcg gtcatttttg gacataactc
    96421 actccctggt ctttatcgtt atataaatgt ttatatattg aatatttagc gcgctaacaa
    96481 tagagggagt ctacccattt tgggttaaga attattaatc cacatcaata gaagggtatg
    96541 agtaatatgg tgggattatg ttgtatgttc aaatcgccgg atgtgtcata tccggcgttc
    96601 agtcgataat gtgttactgc ggttcggcag gcgcgccatc ctgggtagac tgcgcgggag
    96661 cagagacgtt accgctggtg gtgcgggtgt agagaatttt atgcgtatca ttagcacaat
    96721 gcccgacgac ctgggaatca ggctgatcaa cctggtcatt gggtacaata cttaacgtga
    96781 agctgctttc gggtacgcca ttattgataa tgcgctgtga tatatcgctc tgtatgcgct
    96841 cacaggatcc cggcgcggcg agtaccgcgg gtgaggcgag ggcgagcaga agcgcggcac
    96901 agcaggttga gagtttcatc ataagctcct tacgcgaaga taacttcttt aagcatagca
    96961 tttaacgtgt aaagtactgt atttgctact atgattgaga atcatctcta ctctctggtg
    97021 actgttgtga aatacaaatt actaccatgc ttactcgcga tattcctcac aggatgtgac
    97081 cgcacagagg taacactttc atttacccct gagatggcca gtttctctaa tgaattcgat
    97141 tttgatccgc tgcgtggtcc ggtaaaagat ttcactcaga cattaatgga tgagcaaggt
    97201 gaagtgacga aacgtgtttc tgggactttg tcggaagaag gctgttttga ttcactcgaa
    97261 ttactggatc tggaaaataa taccgtggtc gctctggtac tggacgccaa ttattaccgt
    97321 gatgccgaga cgctggagaa gagagtacgt ttacagggaa aatgccagct agcagaatta
    97381 ccttctgccg gggtgagttg ggaaaccgat gataatggct tcgtgattaa agccagcagc
    97441 aaacaaatgc agatggaata tcgctatgat gatcagggtt atccactggg taaaaccacg
    97501 aaaagtaacg acaaaacatt atctgtcagc gccacgccat caacggaccc gatcaaaaaa
    97561 ttagattaca cagcggttac tttactgaat aatcaacgag ttggtaatgt aaaacagagc
    97621 tgtgaatatg acagtcacgc caatccggtg gactgtcagc tgatcattgt tgatgaagga
    97681 gtaaaacccg ccgtcgaacg ggtttacacc atcaaaaata cgatcgatta ttattaatgc
    97741 tattgtgcgg tcggcttcag gagagtctga cccggtgttt tgtgctctgc cagatactga
    97801 tgctggaata tacacatgcg aatggcatta cgatattgac cattaataaa gaactcatgc
    97861 atcaattcac cttcaaccgt aaagccaagc ttgcggtaaa tgtgaatcgc tttttcattc
    97921 tctttatcaa cgatcagata cagcttatag agattgagaa cggtgaagcc atagtccatt
    97981 gctaatttgg cggcacgggt tgccagacct ttcccctgat actccgggga gataattatc
    98041 tgaaattctg cgcggcgatg aacatggtta atttccacca gctccaccag accggctttt
    98101 tcgccgtcac attccaccac aaagcgccgt tcgctctgat cgtgaatatg cttatcatac
    98161 agatcagaga gttcaacaaa ggcttcgtag ggttcctcaa accagtaacg catcacactg
    98221 gcgttattgt cgagttgatg tacatagcgt aaatcttcac gctccagcgg gcgtagctta
    98281 acactgtggg cgcttggcat aacgtgtcct tacattcctt aaatcaataa caggttaggg
    98341 ggtaataacg cggccagttc gacggtccag gcagcgcaaa gtatttggct cccagtaggc
    98401 attgatgttg gcgctttgct cgcatttatc gcggttatca aaagcggcgt cggctttatc
    98461 ccactctttt tcagtgcgtt tattcacttt ctgacgcaga ttgcgcgtgt cattccattg
    98521 ctctttttcc atagcggcgt gctggcggct ttgtgcactg tcgccagact caatcaccag
    98581 tttgttagtt tcggcatgaa cagttgtgct caatgccagt gcgcaaggca gcagaaaagc
    98641 gagcaggccg attcgtttgc tgagagtgat tttcataatt cattccctgt gtgaatgatt
    98701 aaaggtgatt ctacaccatc cactgcggac gcaaaacgta ccaggagggt gtttatattg
    98761 atgatattat gtcgccctat aactatacat gatgtcaata agtgacaaag atgattaaaa
    98821 caacgttact attttttgct actgcgctgt gtgaaattat tggatgcttt ctgccctggt
    98881 tgtggttaaa acgaaacgcc agtgtctggc tgttgcttcc ggcggggatt tcactggcgc
    98941 tgtttgtctg gttgttaacg ttgcatcctg cggcgagtgg gcgtgtttac gcggcttatg
    99001 gtggcgttta tgtctgcacg gcgttgatgt ggctgcgcgt tgtggatggc gtgaaactga
    99061 cactttatga ctggacgggt gcgttgattg cgctttgcgg catgttgatc attgttgcgg
    99121 gctgggggcg tacgtaggaa cataaatcta ttttatcaat aagatacgag gaagcgtcag
    99181 ctgacaaaag gtattctatt tcatcttttg tcaaccattc acagcgcaaa tatacgcctt
    99241 tttttgtgat cactccggct tttttcgatc ttcatacttg tatggtagta gctcagttgc
    99301 gtagatttca tgcatcacga caagcgatgc aaggaatcga acatgaagat cgtaaaggct
    99361 gaagtttttg ttacctgtcc ggggcgtaat ttcgtcacat taaaaatcac cactgaggac
    99421 ggtattacgg gccttgggga tgccaccctc aatggacgtg agctttccgt ggcctcttat
    99481 ttgcaggatc acctttgtcc gcagcttatt ggtcgcgatg cgcaccgtat cgaagatatc
    99541 tggcagtttt tctataaagg tgcttactgg cgtcgcggtc cggttacgat gtcggccatt
    99601 tcagcggttg atatggcgct gtgggatatt aaagccaaag ctgccaacat gccgctttac
    99661 cagttactcg gcggcgcgtc tcgtgaaggg gtgatggttt attgccatac caccggtcac
    99721 agtattgatg aagctctgga tgattatgcc cgtcatcagg agctgggatt caaagccatc
    99781 cgcgtgcagt gcggaatccc tggtatgaaa accacctacg gcatgtcgaa aggtaaaggt
    99841 ctggcttatg aacccgcaac caaaggacag tggccggaag agcagctgtg gtcgacggag
    99901 aaatacctcg atttcatgcc gaaattgttt gacgcggtac gtaacaagtt tggttttaat
    99961 gaacatttgc tgcatgacat gcaccatcgc ttaacgccta ttgaagcggc gcgctttggt
   100021 aaaagcattg aagattatcg catgttctgg atggaagacc cgacgcctgc ggaaaaccag
   100081 gaatgcttcc gtctcattcg ccaacatacc gtcacaccca tcgcagtggg tgaagtcttc
   100141 aacagcatct gggactgcaa acaactgatt gaagagcaac tcatcgatta tatccgcacc
   100201 acgctgaccc atgcaggcgg aattaccggt atgcgccgga ttgccgattt tgcttcgctg
   100261 tatcaggtac gtactggctc acacggtcct tccgatttgt caccagtctg catggctgcg
   100321 gcgctgcact ttgatctgtg ggtccccaat ttcggtgtcc aggaatacat gggttattcc
   100381 gaacaaatgc tcgaagtctt cccgcacaac tggactttcg ataacggcta tatgcatccg
   100441 ggagacaaac cgggtcttgg tatcgaattc gatgaaaagc tggcggcgaa atatccctat
   100501 gaacctgctt atctaccagt cgcacgtctg gaagatggca cgctgtggaa ctggtaagga
   100561 gtaagataat gaaaagcata ttaattgaaa aaccgaatca actggcgatt gtcaaacgtg
   100621 aaatacccac cccgtcagcg ggtgaagtac gagtaaaagt gaaacttgcc ggaatttgtg
   100681 gttcagatag ccatatttat cgtgggcata atccttttgc gaaatatccg cgcgtcattg
   100741 gacatgaatt ctttggcgtc attgatgcgg tgggtgaagg tgtggaaagc gccagagtcg
   100801 gtgaacgtgt tgctgtcgat ccggtggtca gctgtggtca ttgctatccg tgctctatag
   100861 gtaagccgaa cgtttgtacg acactcgctg tattaggtgt gcacgttgac ggtggtttca
   100921 gtgaatatgc tgtggttccg gcaaaaaatg cgtggaaaat tcctgaagca gtggccgatc
   100981 aatatgcagt gatgatcgaa ccttttacca ttgcggctaa cgtaaccggt catggtcaac
   101041 cgactgaaaa tgataccgtt ctggtttacg gtgccggtcc aatcggcctg acgatcgttc
   101101 aggtattaaa aggcgtctat aacgttaaaa atgtgattgt tgccgatcgc attgatgaac
   101161 gactggaaaa agcgaaagag agcggggctg actgggtgat taataacagc cagacaccgc
   101221 ttggcgagat tttcgctgaa aaaggcatca agccgacatt aattatcgat gcggcttgtc
   101281 atccttctat cctgaaagag gccgtaacgc tggcttctcc agcggcacgt attgtattga
   101341 tgggcttctc cagtgaacct tctgaagtga ttcagcaact aattaccgga aaagaactct
   101401 ctattttctc ttcacgctta aatgcaaata aattcccggt cgttatcgac tggttaagta
   101461 aagggttaat tgaaccagaa aaattaatta cccatacgtt tgatttccag catgttgctg
   101521 atgccattag tttatttgaa caggatcaaa aacattgctg caaagtctta ctcacttttt
   101581 ctgaataata ccaataacgg cgagtaagta gtacgcatct tacctctttt ttagagataa
   101641 ccattatgac aatagaaaaa cacgaaagaa gcactaagga tttggtgaaa gcagcagtat
   101701 cgggatggct gggcactgcg cttgaattta tggatttcca gttatattcg ctcggcgcag
   101761 cgttagtgtt tcatgaaata ttttttcctg aatcatcaac ggcaatggcg ttaattctgg
   101821 caatgggaac ctacggtgca ggttatgtgg cgcgtattgt cggagcattt attttcggca
   101881 aaatgggcga cagaataggg cgtaaaaaag tgctctttat taccatcacc atgatgggga
   101941 tctgtaccac cttaattggt gtgttaccga cctatgcaca gattggtgtt tttgccccca
   102001 tcttgctggt gacgctgcgt attattcagg ggttgggtgc aggtgcggaa atttccggtg
   102061 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
   102121 tagctatggg aactaactgc ggaaccttga gcgcaacggc aatctgggcc tttatgttct
   102181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
   102241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtttttg
   102301 agaaggttaa cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
   102361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
   102421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
   102481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
   102541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tccgcgattg
   102601 tgctggcatg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
   102661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
   102721 ttaccatggc agaaatgttc ggctgtaaaa accgctttac ccggatggct atttctaaag
   102781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
   102841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
   102901 tctctgcgct gaaaatgcca gaagtgaaag accgtgattt aagtgcgctg gaagacgctg
   102961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaatcctca
   103021 atcccttctc ttaccggaga ggggattttt tattcatata acaaaacatg tagcttgcca
   103081 tatttatatt taaggaatag ttatgggaaa taatttgtta tcagcaaaag cgacgctccc
   103141 tgtttatgat cgtaataacc tggccccaag aattgttcat ttaggctttg gtgcatttca
   103201 ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca gaatatttca gtgactgggg
   103261 atattatgag gtcaacttaa tcggcggcga acagcaaatt gccgatttac aacagcaaga
   103321 taatctttat accgttgcgg aaatgtcggc cgatgcgtgg acggctcgcg tcgttggcgt
   103381 cgttaaaaaa gccttgcacg tacagataga tggcttagaa accgtgttgg ctgcgatgtg
   103441 tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa aaagggtatt tccactctcc
   103501 ggcgaccgga cagttaatgc tcgatcaccc gatggtcgtt gccgacgtgc aaaatcccca
   103561 ccagccgaaa acagcaacag gggtgattgt tgaggcgctg gctcgccgta aagcggcagg
   103621 acttcccgca tttaccgtca tgtcatgtga caacatgcca gaaaacggtc atgttatgcg
   103681 tgacgttgtc acttcctacg cgcaagccgt tgatgtaaaa ctggcacaat ggatcgaaga
   103741 taacgtgact ttcccatcaa caatggtgga ccgtattgtg cccgcagtga cagaggatac
   103801 gctggcgaaa atcgaacaac ttaccggtgt gcgcgatcct gctggcgttg cctgtgaacc
   103861 tttccgccag tgggtaatag aagataattt tgtagccgga cgtccggaat gggaaaaagc
   103921 gggagctgaa ctggttagtg atgtgctgcc ttatgaagag atgaagttgc gcatgctcaa
   103981 cggcagtcat tcattcctgg cgtatttggg ttatcttgcc ggatatcagc acattaatga
   104041 ctgtatggaa gatgaacatt atcgttatgc ggcgtatggc ttgatgttgc aggaacaagc
   104101 gccgacgctg aaagtgcagg gcgttgattt gcaagattac gctaaccgat taattgaacg
   104161 ctatagcaac ccggcgttac gtcatcgaac ctggcagatt gcgatggatg gcagccagaa
   104221 attgccacag cggatgttgg attctgttcg ctggcatctg gcgcatgaca gcaagttcga
   104281 tctgctggcg ctgggcgtcg cgggttggat gcgttatgtg ggtggtgttg atgaacaggg
   104341 aaatccgata gaaatcagtg acccactgtt acctgttatt cagaaggctg tacaaagtag
   104401 tgccgaaggg aaagcgcgcg tccagtcatt gctggcgatt aaggcgattt ttggtggtga
   104461 tttgccagac aatagcttgt ttactgcaaa agtgacggaa gcgtacttgt ctttattagc
   104521 gcatggcgcg aaagcgaccg tggcaaaata ttccgtgaag taaaaaaagc aatgccgcca
   104581 tcggcggcat tcacacgctt agttcatgcc gagacgaatc aggtccagtg gtgccagttt
   104641 ctcttcacaa ccttcaacaa ctacggtttt tccgcggcgg atttgctcag cggtcagacc
   104701 ttcagtatca atcgacagga tcttgccagt gtgaccggtg ccgctaacca taacacggct
   104761 gccagtggtg attgcgttac ggttacgatc gtaggtcgtc ataggtgttt tctcctttct
   104821 gatttacagt cgaagcccac gtaacgggcg caatataaat actcctgtcg agtgaaatat
   104881 tttttgtttt tgatcaagct cacatctttt ttgatgcatg catgagccat aaggcggggg
   104941 taaagactct caccccggat gggatgagag gaaatgaatt actcttcgct aaaccagtcg
   105001 ctgttttcct ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt
   105061 gcacgttcta ccgcatcgcc atcacgtttt tgcaaggcag agaaaatatc aagatgctgg
   105121 cgtaacagca tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg
   105181 gtcgctttca ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg
   105241 agtagatgga agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca
   105301 atgcgttgct ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc
   105361 gccgcccgac gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc
   105421 tgggccatgg aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg
   105481 ttttccgcca gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca
   105541 gaaacttctt tttccgacaa cggtgtgcct ggggcaatca ggcaatggac aatgtcgcga
   105601 cgaaggatac gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg
   105661 gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa
   105721 gcaataaccc ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc
   105781 atagctttgg gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt
   105841 tgacacccac cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata
   105901 ggttttttcc gctttactgt catcgccttt aaagcggaca ttggaaaact cggtgccacc
   105961 taccagaccc ggttcgatgt cggtgacgcg caccgccgta ccatgcaggt ccgtacgcag
   106021 attcaggcta aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata
   106081 cggccagcta cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat
   106141 acccggtaag acggcgcgcg tcatatatac aaggcctttg ttgttggtat caatcatcgt
   106201 ttcccagtct tcaatgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt
   106261 atttaccagg atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat
   106321 agcggcgcgg ttgcgaacgt ccagttgggc gatgtacaga ttatctccca gttcgtcttt
   106381 taactcctgc aaccgttcct ggcgacggcc agtggcgata actttatgcc cttgttgaat
   106441 aaaacgacga gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat
   106501 ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg
   106561 taatactggt ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg
   106621 cgttcaggag aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta
   106681 tctggctcct cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg
   106741 aatacagcaa aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cacctgattt
   106801 caacaatact attctggcac tggaacaaag tggagaatta cttacccgcg ttaccagcgt
   106861 cttttttgcg atgactgcgg cgcataccaa tgatgaattg cagcgtcttg acgagcagtt
   106921 ttccgctgaa ctggcggaac tggctaatga tatctatctg aatggtgaat tattcgcgcg
   106981 ggtagatgct gtctggcaac gccgtgaatc tctggggctt gatagtgaat ccatccgcct
   107041 ggtggaggtg attcatcaac gttttgtcct tgccggagcc aagcttgcgc atgctgataa
   107101 agcaaaatta aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg
   107161 attactggca gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg
   107221 aatgagtgag caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa
   107281 caaatggctg attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga
   107341 tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc
   107401 caatgatacc cgcgctatca ttcaacgtct ggtggagatc cgtgcacaac aggcaacatt
   107461 acttggtttt cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacacctga
   107521 agcagcactc aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga
   107581 attagcctcc atacaggcgg ttattgataa gcagcagggc gggtttagcg cgcagccgtg
   107641 ggactgggca ttttatgccg aacaggtccg gcgagagaaa tttgatcttg atgaggcgca
   107701 gctcaagcca tattttgaat taaacacggt gttgaatgaa ggtgtattct ggaccgcgaa
   107761 tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt cctgtctacc atcctgacgt
   107821 tcgggtgtgg gaaatttttg atcataatgg cgtggggctg gcgttatttt acggtgattt
   107881 cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc aattttgttg aacaatcaac
   107941 gcttaatgaa acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg
   108001 tgagcctgcg ttgttactct gggatgatgt cataacctta ttccatgaat ttggtcatac
   108061 gctgcacggc ctttttgccc gccagcgttt tgccacgctt tccggtacca acacgccgcg
   108121 tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt
   108181 cgctcgctac gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa
   108241 aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc
   108301 acttctcgat atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga
   108361 ttttgaattg cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta
   108421 tcgcagcagt tatttcgccc atatttttgg tggcggatat gccgcaggtt attacgctta
   108481 tctgtggacg caaatgttgg ccgatgatgg ttatcagtgg tttgttgagc agggcggatt
   108541 aacgcgtgaa aatgggctgc gttttcgcga ggcgatcctt tccagaggta acagcgaaga
   108601 tctggaacgc ctgtatcgac aatggcgcgg taaggctcct cagattatgc cgatgctgca
   108661 acatcgtggc ttgaacatat aaacaccacc aatggctgga ctggagatat cgccagtcca
   108721 gcctggttat agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa
   108781 taccgtttcg cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg
   108841 ccacgcgaac cagacggtac ccgctggcgt accatcttca cctccctccg gtccgccgta
   108901 gccggtaatg gcaatactga catcagcatc cgcacgctct atggcaccgg ttgccatttc
   108961 tgctgccact ttctcactca ccgcagaata tcgttcaaga gattgctggc ttacgctgag
   109021 gattttcatc tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt
   109081 atcttcagct gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt
   109141 taagcgtaat tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt
   109201 gtcggtatct gcaagttgta caattttatt tctattaatg ttcactgttt tttatccacg
   109261 cttttatatg taaaggtgat ttgctttttc tgtatgctcg tccaatataa taatttccaa
   109321 acatataaat ccgattattc tgaaaatatg tttaagactt ttcaatcttc ttaactttag
   109381 tgagttaaac tctctgaata tttgacagct agttggctga ttttaaatgc cttataacaa
   109441 ttggcagtat tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca
   109501 aacgcaatta aggagtcagt atgaaatttc agcccattgt attagcaagt tttcttgtca
   109561 tgccttatgc gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgt
   109621 ctcatgcaat agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt
   109681 tcgcaaaaac catgcacgat ggcgcttcgg tttcactacg cggtaatttg atttctcaca
   109741 aaggagaaga ccgttacgtt tttcgcgata agagcggtga aattaatgtc gttattcctg
   109801 cggccgtttt tgacggacga gaagttcagc cggaccagat gatcaacatt agcggcagtc
   109861 tggataagaa aagcgcgccg gccgtcgttc gggtcactca tttgcagaaa taaaaataaa
   109921 ttagcctgat ggcctgaact accgggccat ttgggtactg ggttatgcta tttgtcatcc
   109981 tttcatttgc gataatgcag aggtaattta cagaattaaa cactttttta tccgctgatt
   110041 ttgttttaaa tcacaatgtt atctcagaga tgaatcataa cttcggattt ctatgaaaat
   110101 attgacgtaa ttttgctttt ctctcgttag aatagcgcgc acaaggaact gtgaaaaagg
   110161 agtggcaatg atcaagaaga caacggaaat tgatgccatc ttgttaaatc tcaataaggc
   110221 tatcgatgcc cactaccagt ggctggtgag tatgtttcac agcgtggtcg cgagagatgc
   110281 cagtaagcca gaaataacgg ataaccattc ttatggactg tgccagtttg gtcggtggat
   110341 tgatcatctg gggccactcg ataacgatga attaccttac gttcggctaa tggattctgc
   110401 ccatcaacat atgcataact gtggtcggga attaatgctg gctattgttg aaaatcactg
   110461 gcagggcgcg catttcgacg cctttcagga agggttactt tcttttactg cggcattaac
   110521 cgattacaaa atttatttac tgacgatccg tagcaatatg gatgttttga cgggattgcc
   110581 ggggcgtcgg gttcttgatg aatcctttga tcatcagtta cgcaacgctg agcctctgaa
   110641 tctttattta atgttgttgg atattgaccg atttaaattg gttaatgata cctacgggca
   110701 tttaatcggc gatgtagtat tacgcaccct ggcaacttac ttagccagtt ggacgcgtga
   110761 ttacgaaacg gtttatcgct acgggggcga agaatttatc attattgtca aagcggctaa
   110821 tgatgaagaa gcatgtcgtg caggtgtcag aatttgccag ttagtcgata accatgccat
   110881 cacacattct gaagggcata tcaacattac cgtgacagca ggtgtcagtc gcgcatttcc
   110941 tgaagagcct ctggatgtgg tcattggaag agcagaccgg gcaatgtatg agggtaagca
   111001 aaccggaaga aatcgctgca tgtttattga cgaacaaaat gtgattaacc gagtttaagc
   111061 tccgtttaac attcattgag aaaactgatg ctactgtgtc aacgatccgt tatcagtgca
   111121 ggaaaatgcc tgttagcgta aaagcaaaac acaaatctat ccatgcaagc attcaccgcc
   111181 ggtttactgg cggttttttt tcgccgtcat aaaaatcagg ccccttgtac acaactgtaa
   111241 caaggggccg gttaggtgag ggattatctc cgttcattag tcatcccatt tgtggctgaa
   111301 atacgcggcc agaaaaccag aaaataaaat tattcccagt acggccataa aaacattcat
   111361 attacccagc attatttgcc tccgtttatg gtgtggtgtg tttcctcgct taccttaatt
   111421 ttcatctcaa tgagaaagat attagcagag gtaacctaaa cgaaggctga attaaatttt
   111481 tattgtgttc gacttaaatc aacaaagcgc gggctgcccc cacggttttg cacgaatccc
   111541 ttttaacatc agcacccacg cagcaatgat caccaacgct aagatgataa acagcgagga
   111601 aggcggcagg ttggttagca ctaggccact cactaatggg ttaattgcgg caccaagcca
   111661 gcctaaagac tgggcggaaa aatagctggc tttcattcct ggcggcgcaa tatggtcaat
   111721 caacatatac tcgcccggcg cataaattat ttcaccgaca gtaaataccg cagctgacat
   111781 accccacaat agcaggctgt ttccggaaaa aataaaaccg accagaccga tgacgaaaca
   111841 gagggtgcct gctgtcatca gcgcgcggat gttagccggg ttaagtcggc ggcccacgga
   111901 atattgcaac gtaactacca tggcggcatt caccggaaga acaaccgcga ccaccttttc
   111961 ggcaaaatca ccatcagcaa tcaccatcac atattgtgaa atgcatgaag caaatgcgcc
   112021 actaacaaaa gaagccagaa agccagagca ggtaaaccac aacagtgcct tatcttgtaa
   112081 taaaactttc ggcgaccaga cactgcctgt ttccgtggcg atgattttct cgctgcgctt
   112141 tacccaaatt tgaatgaaaa acatgggaaa cgcagagcag atagcggcca gccaaaaggg
   112201 cagattgatg ctctgcatga ccaacagcgt gccgagtggc gggccgacgg tccagccaat
   112261 attgagcatg gtgtagttga tcgagaagat cttcgttttg ctggtggatg aaagattgtc
   112321 ggcaaaccag gctttcaaca ctgtggcaaa aacggaatag gcgcagttga tgagtgcgaa
   112381 aaaaagtacc accagcgtga cgctgtccac caggggaata gcaataaaac cgctggcgaa
   112441 ggccgtaatt gccagtaaca tatagcgttt cttgtcgaac ttatccgcca ggataccaaa
   112501 accgaggcta aaaacgacgc caatagtgag cgcaattgtc atcgcataac caatgagatc
   112561 gacactcagg ctgtactgac gacttaagta aatggtcata aatggcagcg tagcgccgcg
   112621 tccgatggtt aataacaacg acgaggcaag aagggcgctg gtagagcgtc gtagggataa
   112681 gttcatttgt ctgtccagca gtgcgtttcg ttatgttttg ttttagagat gtaaagaaat
   112741 agcatggcaa aaatccgaag tatagcgact tatttgcgca ttaatgccgt cgtaaccggc
   112801 gactaacgaa tcaactgtta aagaaaagta aattcagcta agttgattgc ttacaaaaga
   112861 tgaaattcag aggcggtaga atgtcgcgaa aagatggggt gttggcgcta ctggtagtgg
   112921 tcgtatgggg gctaaatttt gtggtcatca aagtggggct tcataacatg ccaccgctga
   112981 tgctggccgg tttgcgcttt atgctggtcg ccttcccggc aatctttttc gtggcacgac
   113041 caaaagtacc gctgaattta ctgctggggt atggtttaac catcagtttt gcacagtttg
   113101 cctttctctt ctgcgccatt aacttcggta tgccggctgg actggcctcg ctggtcttac
   113161 aggctcaggc gttttttact atcgtgctcg gtgcttttac ttttggcgaa cggctgcacg
   113221 gtaagcaact ggcggggatt gcgttagcga tttttggcgt actggtgtta atcgaagata
   113281 gctttaacgg tcagcatgtg gcgatgctcg gttttatttt gactctggca gcggcattta
   113341 gttgggcgag tggcaatatc ttcaacaaaa agatcatgtc tcattcatcc cgtccggcgg
   113401 taatgtctct ggtcgtctgg agcgcattaa tcccgataat tccgtttttt gtcgcctcgc
   113461 tcattctcga tggctccgga aaaatgattc acagtctggt cactatcgac atgaccacca
   113521 ttctggcgtt gatgtatctg gcatttgtag cgacgatagt tggctacggg atttggggta
   113581 cgctgcttgg acgctatgaa acatggcgtg tcgcaccatt atcattactg gtgccggtag
   113641 tcggtctggc aagtgcagcg ttactgctgg atgaacgctt aacaggtctg caattcttag
   113701 gggctgtgct cattatggcc ggactgtata tcaatgtatt tggtttgcag tggcgtaaag
   113761 cggtgaaggt aagaggttaa caagccccga tatgccgggg cctgaatata ctacatggct
   113821 tgttggttat aatagggcac gcccagcgca tccgatttgt cactgcctgt acccatgtga
   113881 tttaaatcga acggtggttg ttcctgcgat gggggaacaa ccaccacatc gccacaggaa
   113941 gtaacgagcg gttgctgggt ttgttccgca acgccctgcg cggaaaagag aataagcgca
   114001 gctgctattg cggataaaag cggtttcata attgcctcag tgacgttgtc acgttttcaa
   114061 ctagctgttg taatggttta atggatgtaa aaagcgcgat tcgccctgca tattggtcat
   114121 ccggtattta tgcggcggaa catcaaagta atttttgaag gttcgggtca gagtttgttg
   114181 cgactcgaag ccatatcgtt ctgccagata gagtatcggc tcgttacttt ccttcagctt
   114241 ttgcgcgatt tccgtcatct tacggctgcg gatgtattgg cctaatgaat gaccggtttc
   114301 ttttttaaac atccgttgca ggtgccattt agagtaaccc gaacgctctg acactttctc
   114361 cagtgacagt ggcgattcca ggttgtcctc gatccagtcc aaaatgctat gaatggtaat
   114421 agcgtcagta ttgcgtctgg acatcgtcat acctcttttt tgtttacggc aggactttct
   114481 taagcaaatg ctcaagtgtt gccacttcgt ccgccgtcag gttttttgtt aattcttgat
   114541 gcaggtcctg gccaactaat tgatggcatt gttcacatat tgccgcgccg ctggtggtaa
   114601 gttttaccag tacgccgcgc ttatcattcg ggttcggcaa cctttctacc cagcctttac
   114661 agaccaggcg atccagcata cgggtcagtg ctcccaggtc gaccgacaac acttttttca
   114721 gttcaaccgg agtaatacac gccgcgcagc ggatagagca gagcacctta aactgtgccg
   114781 cggtaatatc cagcggagac agatactcgt taagcagacg atctttcttc tgattaacca
   114841 tatggattaa gcgacccaat ggaataattt cattgaacag atcgctggta cttttcacat
   114901 tagttgccct ggcaagtaat tagttgcagg ggataatatt gcccaggcaa gtataagtca
   114961 aatgaatgaa ttgaccgatg ccacgttttg ctaaatcggt tcaagttttg gtcaatgcga
   115021 tgcaaggtta gctaaagagt gacactttat ccttgtttac cccttatact tttcgctgat
   115081 aacccagata cacaggataa caaccaccaa tgttagattt gtttaaagca attggcctgg
   115141 ggctggtggt gttactgccg ttagctaacc cattaacaac tgtcgcgtta tttcttggcc
   115201 tggcaggcaa catgaacagt gccgaacgta atcgtcagtc gttgatggcc tcggtgtacg
   115261 tatttgccat catgatggtg gcgtattacg ccgggcaact ggtgatggat acatttggca
   115321 tttcgattcc cggtctgcga attgcaggcg gcttaatcgt cgcgtttatc ggttttcgga
   115381 tgctctttcc gcaacagaaa gcgattgatt caccggaggc gaaaagcaag tcggaagagc
   115441 tggaagatga acccagtgcc aatattgctt ttgtaccact ggcaatgcca agtaccgccg
   115501 gtccgggaac cattgcgatg attattagct cggcgtccac agtgcgtcag agttcaacct
   115561 tcgctgattg ggtactgatg gttgcgccgc cgctgatctt tttcctggtg gcggttattt
   115621 tgtggggaag cctacgcagc tccggcgcaa ttatgcggct ggtgggcaag ggtggcattg
   115681 aagcgatctc ccgcctgatg gggttcctgc tggtatgtat gggtgtgcag tttattatta
   115741 acggcatcct ggaaatcatt aaaacgtatc attaaaaaag gcccgaatgg gcctttcaac
   115801 tattgcgtct gttcttcgag tgtcactggc cagcggcgaa atataatgat tgaccatatt
   115861 aacgcggcaa aagcaggcac cgcgcccaca taaccaatca tcgacattga ccagtgcaga
   115921 ctcacctgat tacctaccaa cgcacccgcc ccgattccaa tattaaatat cccggagaat
   115981 agcgccatcg cgacgtcggt ggcatctggt gccagcgcca gcactttaac ctgcatacca
   116041 agcccgatga tcatcatcgc gatcccccag aaaatactca gcaccccgag gtgtatttca
   116101 ctgttcgccg caggtagcag cagtgccaga cacaccagca acagcgcaat cgccgtactc
   116161 accaacgcag acgcatactg attacccagt ttcccgaaaa tcacgctgcc aataatgccc
   116221 gcaccaccga gtaataacag taatgccgtg gcaaagttgg cgctgaatcc cgcaatgttt
   116281 tgcacaaaag gctcaatata gctgtatgcc gtgtaatggg cggtgacaac caccacagtt
   116341 aacaaataaa tgctcatcaa tgccgggcgg cggaacaata gcgggaggct tttcaatgaa
   116401 ccggaatgct cactgggcag taagggaagt aacttaatca ggcacaaaag ggtaacaagc
   116461 gccccgatac caatcgcgaa gaaggtcatt cgccaaccga aatactgccc cacaatgcgc
   116521 ccgagaggta atcctaatac cattgccagt gctgtacctg tggcaattaa actcaatgcc
   116581 tgtgctcgct tcccggccgg agccatacgg atcgccaggg acgccgtaat cgaccagaaa
   116641 atcgcatgtg caaaagccac accaatgcga ctgatcacca gaacggtaaa gctccacgat
   116701 aaaaacgaca gtacgtggct ggcaataaac accacaaaca ggcagatcag taatttgcgc
   116761 cgttcaacct ggctggtcat tagcataaaa ggcaatgaca ttagcgctac tacccatgcg
   116821 taaatggtca acataatgcc gacctgagcg gtttgcatgt gaaaactttg cgcaatgtca
   116881 gagagtaggc caacagggac aaattcggtg gtgttgaaga tgaaggcggc gactgccagc
   116941 gtaacgaccc gtagccacgc cactttgcgg gaaacagtgt ttgttgtcat aacagattat
   117001 cgggtgagtt gcgaaaaagt gatgaaatga tcttaaaacg gttaatgatg tgaatacaat
   117061 gcatttgtga tctggatcgc gttttccgtt tctctgacgc tgattctttc acgcgtcaga
   117121 gagacggtaa acatcacggt ttaatgacga tcaggaatgt ctggcgtttt ggaggttgcg
   117181 ggtataggtg gtctgacagg ggagcgaggc ctgtttttca ccaggtcttc atgagtaact
   117241 tttggcggtg tttcattttt tacaaaaagt ttgagggtct tgcgcgccat atgtttgata
   117301 attttatcat cacttaatga tgctggcatc aacacgttat tcaaatcact ttcattggga
   117361 ttcataaatt ggtgatagtg caataacata ctggcgaaaa ataaaaacaa gtcctgaaaa
   117421 tgaggagatt cttgttctgc taaaagggat aaaaagcaga tgcagacttc gggtagtaca
   117481 tgtttgttag aacctaatga atccatcagg ctttgcaata aatcctcttt taattcgctg
   117541 tccgttatgt cgagaatggc ggtgatcaag ttaggatctt tattgacgaa ccatctgtga
   117601 acctcagagt tattccataa tcgggggagt ataacaaaat taccgcgaga gataaaattc
   117661 aggacaagca aatcaaactg gcgtaagtaa tctgcagcgg tatggtgatc cggaggcagg
   117721 ggttcaatat gtggtgtatg cgctcttttg atgggaacgc cagggtttat cattggaaat
   117781 tattcaataa aatcattcag aaacagaaag agcacacgct cgacgccggt ctgaaatacg
   117841 cgcccca
//
LOCUS       DAPGRN010000002       109079 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPGRN010000002 DAPGRN010000000
VERSION     DAPGRN010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.002"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(37..318)
                     /locus_tag="RHW33_000117"
     CDS             complement(37..318)
                     /locus_tag="RHW33_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV1643673.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCNMAMV
                     NSQPQQHCGQLTYNVDTRHQFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(581..1690)
                     /gene="apbC"
                     /locus_tag="RHW33_000118"
     CDS             complement(581..1690)
                     /gene="apbC"
                     /locus_tag="RHW33_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV1643674.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1822..3855
                     /gene="metG"
                     /locus_tag="RHW33_000119"
     CDS             1822..3855
                     /gene="metG"
                     /locus_tag="RHW33_000119"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV1643675.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3996..7793
                     /locus_tag="RHW33_000120"
     CDS             3996..7793
                     /locus_tag="RHW33_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV1643676.1"
                     /translation="MRHFIFQDEKLHKFWAVEQQGNELHISWGKVGTNGQNQVKSFAD
                     AAAAAKAELKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IPVTDDINRFAFPHRSRPREINYLYKDGQIWKRIANNTRAYDPDNNYRSYPENWQQAF
                     AELQMRVKGNQPTGSAQSDAALLWGFWNSYSQDELVDDLVIRCGLESAVEIALFALQL
                     KYKPVKGAVTTIIPPNHEAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAIPSLSPA
                     RQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTTAEKYDFPPLYHD
                     FRAYLAMLLANNGVRGVSRILLEFTEEHSDNPTYLFERISETEDLVKWLWKTNHPDAI
                     QILILGVIGKKKHLEYLSKACQKHPAAALAAYATLLAIHEDKEWRKALVKLITATPGL
                     VTDVIPRVNAKAAGILSECRTQLVAEECEYATADMLPELFVAPPWVINKKTNVIPVFD
                     LPVLSIPAVTDITPGITELISHTDISRFSEIAQYQSSQQTLFTDLPPIKKERWETSFI
                     PLTPEQQILWRLGFKEWRRTGEEQYEKKIMPQSAVDALLRFDFPALKAEFAQYHNKGS
                     RHWQLYALCFLPTQHAISFLNQIINEEQFSGEREILAIFGDAAIPAFMKCLQRKPQQL
                     WIFTLFLGVSELALPMAQRLQKKMSAEDARKWLANFPRHAAAGLLPVALGKKGKDRDC
                     ARQALRLLVNLNQRETIEEIAQGYNQPDVLAALATLFNSEPLEEYPAKIAPLPGFYQF
                     TLWRRPRLKSNNLPLSDDAMRHLGTMLSFPRDITAYAGLDIIREIFTRESLAEFGWDL
                     YTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVSGLDVLADI
                     GSDVALMLLNGIARKIKFKALQEHAREKINIVAENRGLTMAEMEDRLAPDLGLDSSGS
                     LILDFGPRQFTVGFDETLKPVVRDANGKVLKDLPKPNQSDEKTQATDAVNLFKQLKKD
                     VRAIASQQIDRLEQAMCQRRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYNDENALITC
                     FRVAEDSTYSDAQDELFTLPAGNIGIPHVLEIPPESAAAFRQIYVDYELLPPFQQLER
                     GSYHLADNERNVHELSRWNGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGALE
                     LETEPFSLIYGETGYSDLLPVESVKITAPYDRYGKQSSPTFSVLDDITASELINDIES
                     LFD"
     gene            7806..10838
                     /locus_tag="RHW33_000121"
     CDS             7806..10838
                     /locus_tag="RHW33_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV1643677.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQRNELHINWGKVGTNGQSQVKSFAD
                     AAAAEKAELKLIAEKVKKGYVEQVDANAHSHDVSAKKVLVTEEKASINHQAATNGLPW
                     LADNDPIILPPNIARHALSHRLWPGEPVKKPTKPEKQNLISRLANRTYHYYKNLTFDY
                     SSCSDEWADAISQAISLIKAQKTEIPARTMAVLTFLEICFCQGWNDETSSELLDEIVS
                     TFGIEYATETVIFWWQIDFDFDYKDEHLTFFINYADSSKDSYRRNNDKFSLRLRKHLS
                     LAEEEVWQNCIAKLLVALPDISLYRQPLIAILMPEIPEVAHEIVHRLHQVADVPQLEM
                     LKLVATDPFTLEILENYQYIDVFNTYGASWSATVLREQGIAALARLASYAEEDNCGDV
                     LKCINHPLAITALIEAADTNKRCYERLIKSAENFPSATIAALAEALVKKDDKRKHTLL
                     VSLLNANPLLVEKLVIRLSSQAANLLQQCQQLLNSRNEDFANSEILPMVLVSPPWETQ
                     NKNNDNSPVGKYPAKIPPLPLFYQPPLWTRPKLKNSSKVLPDNALLHLGEMLRFPQEE
                     ALYPGLLQVKDACTADSLAEFAWDLFTAWLTTGAPSKESWAFTALGVLGNDDTARKLT
                     PLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIA
                     ESRELTVEELEDRLAPDLSLDDNGSLLLDFGPRQFTVSFDETLKPFVRDVSGSRLKDL
                     PKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVARLESAMCLRRRWSPENFQLFLVV
                     HPLVRHLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGDISIGIPHVLE
                     ISPTDAAAFGQLFADYELLPPFRQLHRNSYALTEAERNASELTRWAGRKCPSGRVMGL
                     ANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGFAVGMSPAELSAEQLLSKLWLWE
                     GKAESYGWGSNSTQEAQLSVLDAITASELINDIEALFE"
     gene            10848..14480
                     /locus_tag="RHW33_000122"
     CDS             10848..14480
                     /locus_tag="RHW33_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV1643678.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATDVVMARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQKNHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIHTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQARAVLNSYLLAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQEWLASTESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLM
                     LAFLHRPKETFPLILNFGATELALPVARVWRRFAVQRDLARQWILHWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEHLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTDDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVIGLNILTLMNNDMALIQLHHVSQRAKSSSLRENAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            14541..14858
                     /locus_tag="RHW33_000123"
     CDS             14541..14858
                     /locus_tag="RHW33_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643679.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLHPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            16098..17186
                     /locus_tag="RHW33_000124"
     CDS             16098..17186
                     /locus_tag="RHW33_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV1643680.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17197..19476
                     /locus_tag="RHW33_000125"
     CDS             17197..19476
                     /locus_tag="RHW33_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643681.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEENDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQSWCWKWGLQQAGEQLLKT
                     VLTRLRQHHLPASTADMAAAHLHSMALAQLRGHKLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPFTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            19469..20605
                     /locus_tag="RHW33_000126"
     CDS             19469..20605
                     /locus_tag="RHW33_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1643682.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGSSNLTPPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20602..22605
                     /locus_tag="RHW33_000127"
     CDS             20602..22605
                     /locus_tag="RHW33_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV1643683.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATTRPTEKEE
                     EWQPAIWLEELATLPDATRKRAQVLVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFASEEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGAGIAGEAKL
                     DHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAVERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVTSLPSVSVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPILQQQG"
     gene            22730..23191
                     /locus_tag="RHW33_000128"
     CDS             22730..23191
                     /locus_tag="RHW33_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV1643684.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23232..23702)
                     /locus_tag="RHW33_000129"
     CDS             complement(23232..23702)
                     /locus_tag="RHW33_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV1643685.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVEKS"
     gene            complement(23749..24468)
                     /gene="yehT"
                     /locus_tag="RHW33_000130"
     CDS             complement(23749..24468)
                     /gene="yehT"
                     /locus_tag="RHW33_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV1643686.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24465..26150)
                     /gene="btsS"
                     /locus_tag="RHW33_000131"
     CDS             complement(24465..26150)
                     /gene="btsS"
                     /locus_tag="RHW33_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV1643687.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26372..27103
                     /gene="mlrA"
                     /locus_tag="RHW33_000132"
     CDS             26372..27103
                     /gene="mlrA"
                     /locus_tag="RHW33_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV1643688.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGYDIFPLGI"
     gene            27163..27270
                     /locus_tag="RHW33_000133"
     CDS             27163..27270
                     /locus_tag="RHW33_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV1643689.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27251..27982)
                     /gene="yehW"
                     /locus_tag="RHW33_000134"
     CDS             complement(27251..27982)
                     /gene="yehW"
                     /locus_tag="RHW33_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV1643690.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(27987..28913)
                     /gene="yehX"
                     /locus_tag="RHW33_000135"
     CDS             complement(27987..28913)
                     /gene="yehX"
                     /locus_tag="RHW33_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV1643691.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(28906..30063)
                     /gene="yehY"
                     /locus_tag="RHW33_000136"
     CDS             complement(28906..30063)
                     /gene="yehY"
                     /locus_tag="RHW33_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV1643692.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGFGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSS
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAGLVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFNLLIALLKVKRND"
     gene            complement(30070..30987)
                     /gene="osmF"
                     /locus_tag="RHW33_000137"
     CDS             complement(30070..30987)
                     /gene="osmF"
                     /locus_tag="RHW33_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV1643693.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(31239..33536)
                     /gene="bglX"
                     /locus_tag="RHW33_000138"
     CDS             complement(31239..33536)
                     /gene="bglX"
                     /locus_tag="RHW33_000138"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV1643694.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEEQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33732..35447
                     /gene="dld"
                     /locus_tag="RHW33_000139"
     CDS             33732..35447
                     /gene="dld"
                     /locus_tag="RHW33_000139"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV1643695.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDENGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35486..36418)
                     /gene="pbpG"
                     /locus_tag="RHW33_000140"
     CDS             complement(35486..36418)
                     /gene="pbpG"
                     /locus_tag="RHW33_000140"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV1643696.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36592..37179)
                     /locus_tag="RHW33_000141"
     CDS             complement(36592..37179)
                     /locus_tag="RHW33_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV1643697.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37349..37927
                     /locus_tag="RHW33_000142"
     CDS             37349..37927
                     /locus_tag="RHW33_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1643698.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKIIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(38057..38818)
                     /locus_tag="RHW33_000143"
     CDS             complement(38057..38818)
                     /locus_tag="RHW33_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1643699.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGLMLANPQFNPE"
     gene            complement(38871..40307)
                     /gene="mdtQ"
                     /locus_tag="RHW33_000144"
     CDS             complement(38871..40307)
                     /gene="mdtQ"
                     /locus_tag="RHW33_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV1643700.1"
                     /translation="MNRDSFYPAIACFPLLLMLTGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGITAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLIALTNHQTKSLTLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            40531..40614
                     /gene="yohP"
                     /locus_tag="RHW33_000145"
     CDS             40531..40614
                     /gene="yohP"
                     /locus_tag="RHW33_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV1643701.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(40878..41825)
                     /gene="dusC"
                     /locus_tag="RHW33_000146"
     CDS             complement(40878..41825)
                     /gene="dusC"
                     /locus_tag="RHW33_000146"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV1643702.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPIK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(41977..43170)
                     /locus_tag="RHW33_000147"
     CDS             complement(41977..43170)
                     /locus_tag="RHW33_000147"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HDV1643703.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVCIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDRADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGKQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTKRITLVGDSAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRVYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            complement(43185..43829)
                     /gene="maiA"
                     /locus_tag="RHW33_000148"
     CDS             complement(43185..43829)
                     /gene="maiA"
                     /locus_tag="RHW33_000148"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HDV1643704.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            complement(43838..44539)
                     /locus_tag="RHW33_000149"
     CDS             complement(43838..44539)
                     /locus_tag="RHW33_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV1643705.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDEQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVVPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPITVKIV"
     gene            complement(44555..45583)
                     /gene="gtdA"
                     /locus_tag="RHW33_000150"
     CDS             complement(44555..45583)
                     /gene="gtdA"
                     /locus_tag="RHW33_000150"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HDV1643706.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGGLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNILGCGFAEDYPEEQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRLGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFASRVARTTD
                     STIYHVVEGSGQVIIGNETFSFSAKDIFVVPTWHGVSFQTTQDSVLFSFSDRPVQEAL
                     GLFREARY"
     gene            complement(45595..46953)
                     /locus_tag="RHW33_000151"
     CDS             complement(45595..46953)
                     /locus_tag="RHW33_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HDV1643707.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYAIGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            47080..47988
                     /locus_tag="RHW33_000152"
     CDS             47080..47988
                     /locus_tag="RHW33_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1643708.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            48119..48517
                     /locus_tag="RHW33_000153"
     CDS             48119..48517
                     /locus_tag="RHW33_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV1643709.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            48514..49209
                     /locus_tag="RHW33_000154"
     CDS             48514..49209
                     /locus_tag="RHW33_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV1643710.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            49339..50223
                     /gene="cdd"
                     /locus_tag="RHW33_000155"
     CDS             49339..50223
                     /gene="cdd"
                     /locus_tag="RHW33_000155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV1643711.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            50373..51092
                     /gene="sanA"
                     /locus_tag="RHW33_000156"
     CDS             50373..51092
                     /gene="sanA"
                     /locus_tag="RHW33_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV1643712.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            51095..51334
                     /locus_tag="RHW33_000157"
     CDS             51095..51334
                     /locus_tag="RHW33_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV1643713.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            51528..52766
                     /gene="preT"
                     /locus_tag="RHW33_000158"
     CDS             51528..52766
                     /gene="preT"
                     /locus_tag="RHW33_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV1643714.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLLRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MVADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            52760..53995
                     /gene="preA"
                     /locus_tag="RHW33_000159"
     CDS             52760..53995
                     /gene="preA"
                     /locus_tag="RHW33_000159"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV1643715.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVSAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(54238..55248)
                     /gene="mglC"
                     /locus_tag="RHW33_000160"
     CDS             complement(54238..55248)
                     /gene="mglC"
                     /locus_tag="RHW33_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV1643716.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(55264..56784)
                     /gene="mglA"
                     /locus_tag="RHW33_000161"
     CDS             complement(55264..56784)
                     /gene="mglA"
                     /locus_tag="RHW33_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV1643717.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(56845..57843)
                     /gene="mglB"
                     /locus_tag="RHW33_000162"
     CDS             complement(56845..57843)
                     /gene="mglB"
                     /locus_tag="RHW33_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV1643718.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(58123..59163)
                     /gene="galS"
                     /locus_tag="RHW33_000163"
     CDS             complement(58123..59163)
                     /gene="galS"
                     /locus_tag="RHW33_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV1643719.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNLAM"
     gene            complement(59305..60462)
                     /locus_tag="RHW33_000164"
     CDS             complement(59305..60462)
                     /locus_tag="RHW33_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV1643720.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQIPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            complement(60479..61147)
                     /gene="folE"
                     /locus_tag="RHW33_000165"
     CDS             complement(60479..61147)
                     /gene="folE"
                     /locus_tag="RHW33_000165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV1643721.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            61405..62241
                     /gene="yeiG"
                     /locus_tag="RHW33_000166"
     CDS             61405..62241
                     /gene="yeiG"
                     /locus_tag="RHW33_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV1643722.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNQG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(62273..64253)
                     /gene="cirA"
                     /locus_tag="RHW33_000167"
                     /pseudo
     CDS             complement(62273..64253)
                     /gene="cirA"
                     /locus_tag="RHW33_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(64545..66014)
                     /gene="lysP"
                     /locus_tag="RHW33_000168"
     CDS             complement(64545..66014)
                     /gene="lysP"
                     /locus_tag="RHW33_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV1643723.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(66219..67100)
                     /locus_tag="RHW33_000169"
     CDS             complement(66219..67100)
                     /locus_tag="RHW33_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1643724.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            67199..68248
                     /locus_tag="RHW33_000170"
     CDS             67199..68248
                     /locus_tag="RHW33_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV1643725.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            68322..69179
                     /gene="nfo"
                     /locus_tag="RHW33_000171"
     CDS             68322..69179
                     /gene="nfo"
                     /locus_tag="RHW33_000171"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV1643726.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARTVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            69182..70270
                     /locus_tag="RHW33_000172"
     CDS             69182..70270
                     /locus_tag="RHW33_000172"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1643727.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQSASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLMPMV"
     gene            complement(70326..71576)
                     /locus_tag="RHW33_000173"
     CDS             complement(70326..71576)
                     /locus_tag="RHW33_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV1643728.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMVFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLTGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(71676..72617)
                     /gene="rihB"
                     /locus_tag="RHW33_000174"
     CDS             complement(71676..72617)
                     /gene="rihB"
                     /locus_tag="RHW33_000174"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV1643729.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            72747..73445
                     /gene="yeiL"
                     /locus_tag="RHW33_000175"
     CDS             72747..73445
                     /gene="yeiL"
                     /locus_tag="RHW33_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV1643730.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(73513..74763)
                     /locus_tag="RHW33_000176"
     CDS             complement(73513..74763)
                     /locus_tag="RHW33_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV1643731.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSEMPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(74857..75795)
                     /gene="psuG"
                     /locus_tag="RHW33_000177"
     CDS             complement(74857..75795)
                     /gene="psuG"
                     /locus_tag="RHW33_000177"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV1643732.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(75783..76724)
                     /locus_tag="RHW33_000178"
     CDS             complement(75783..76724)
                     /locus_tag="RHW33_000178"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV1643733.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(77148..78839)
                     /gene="fruA"
                     /locus_tag="RHW33_000179"
     CDS             complement(77148..78839)
                     /gene="fruA"
                     /locus_tag="RHW33_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV1643734.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(78856..79794)
                     /gene="fruK"
                     /locus_tag="RHW33_000180"
     CDS             complement(78856..79794)
                     /gene="fruK"
                     /locus_tag="RHW33_000180"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV1643735.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(79794..80924)
                     /gene="fruB"
                     /locus_tag="RHW33_000181"
     CDS             complement(79794..80924)
                     /gene="fruB"
                     /locus_tag="RHW33_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV1643736.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            81292..82473
                     /gene="setB"
                     /locus_tag="RHW33_000182"
     CDS             81292..82473
                     /gene="setB"
                     /locus_tag="RHW33_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV1643737.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(82470..82724)
                     /locus_tag="RHW33_000183"
     CDS             complement(82470..82724)
                     /locus_tag="RHW33_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV1643738.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            82879..83451
                     /gene="yeiP"
                     /locus_tag="RHW33_000184"
     CDS             82879..83451
                     /gene="yeiP"
                     /locus_tag="RHW33_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV1643739.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            83674..85140
                     /locus_tag="RHW33_000185"
     CDS             83674..85140
                     /locus_tag="RHW33_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV1643740.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIKQAWQQIAQFGAHQALLNTLKI"
     gene            85258..86244
                     /gene="yeiR"
                     /locus_tag="RHW33_000186"
     CDS             85258..86244
                     /gene="yeiR"
                     /locus_tag="RHW33_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV1643741.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLVDSAALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVRKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            86283..86996
                     /gene="lpxT"
                     /locus_tag="RHW33_000187"
     CDS             86283..86996
                     /gene="lpxT"
                     /locus_tag="RHW33_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV1643742.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            87408..87974
                     /gene="mepS"
                     /locus_tag="RHW33_000188"
     CDS             87408..87974
                     /gene="mepS"
                     /locus_tag="RHW33_000188"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV1643743.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            88155..89711
                     /locus_tag="RHW33_000189"
     CDS             88155..89711
                     /locus_tag="RHW33_000189"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV1643744.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGIEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            89793..91607
                     /locus_tag="RHW33_000190"
     CDS             89793..91607
                     /locus_tag="RHW33_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1643745.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMSEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            91608..92702
                     /locus_tag="RHW33_000191"
     CDS             91608..92702
                     /locus_tag="RHW33_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV1643746.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            92702..93727
                     /locus_tag="RHW33_000192"
     CDS             92702..93727
                     /locus_tag="RHW33_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1643747.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNVWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            93729..95318
                     /gene="yejF"
                     /locus_tag="RHW33_000193"
     CDS             93729..95318
                     /gene="yejF"
                     /locus_tag="RHW33_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV1643748.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(95322..95666)
                     /locus_tag="RHW33_000194"
     CDS             complement(95322..95666)
                     /locus_tag="RHW33_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643749.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(96000..97190)
                     /gene="bcr"
                     /locus_tag="RHW33_000195"
     CDS             complement(96000..97190)
                     /gene="bcr"
                     /locus_tag="RHW33_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV1643750.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(97218..97913)
                     /gene="rsuA"
                     /locus_tag="RHW33_000196"
     CDS             complement(97218..97913)
                     /gene="rsuA"
                     /locus_tag="RHW33_000196"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV1643751.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            98063..99823
                     /locus_tag="RHW33_000197"
     CDS             98063..99823
                     /locus_tag="RHW33_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1643752.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRHHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            99948..100232
                     /gene="rplY"
                     /locus_tag="RHW33_000198"
     CDS             99948..100232
                     /gene="rplY"
                     /locus_tag="RHW33_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV1643753.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(100371..101378)
                     /gene="yejK"
                     /locus_tag="RHW33_000199"
     CDS             complement(100371..101378)
                     /gene="yejK"
                     /locus_tag="RHW33_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV1643754.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            101560..101787
                     /locus_tag="RHW33_000200"
     CDS             101560..101787
                     /locus_tag="RHW33_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV1643755.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            101807..103567
                     /gene="pbgA"
                     /locus_tag="RHW33_000201"
     CDS             101807..103567
                     /gene="pbgA"
                     /locus_tag="RHW33_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV1643756.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQLAFARKYSRAAGNVDDQINRVLNALRDSGKLGNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            103642..103718
                     /locus_tag="RHW33_000202"
     tRNA            103642..103718
                     /locus_tag="RHW33_000202"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103676..103678,aa:Pro,seq:ggg)
     gene            complement(103820..106411)
                     /gene="yejO"
                     /locus_tag="RHW33_000203"
     CDS             complement(103820..106411)
                     /gene="yejO"
                     /locus_tag="RHW33_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HDV1643757.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGIAVFAAIEKGGLLEVKDGG
                     LAFAVDQKAGGAIKATTRVMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRS
                     SVTASAKNFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNVDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            106731..107378
                     /gene="narP"
                     /locus_tag="RHW33_000204"
     CDS             106731..107378
                     /gene="narP"
                     /locus_tag="RHW33_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV1643758.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDRGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLHEREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(107413..108465)
                     /gene="ccmH"
                     /locus_tag="RHW33_000205"
     CDS             complement(107413..108465)
                     /gene="ccmH"
                     /locus_tag="RHW33_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV1643759.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGILVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(108462..109019)
                     /gene="dsbE"
                     /locus_tag="RHW33_000206"
     CDS             complement(108462..109019)
                     /gene="dsbE"
                     /locus_tag="RHW33_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV1643760.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
BASE COUNT        26915 a        28809 c        26975 g        26380 t
ORIGIN      
        1 cggctttcta aatgcaatta aaatcaggtt gccaaattaa tcgtagcgat ttaacacgat
       61 tcgacgtttg ctatcactac ctgcaacaac cacttctgtc gttacacctt tgacaggcga
      121 agaaaactga tgacgggtat caacattata cgttaactga ccacaatgct gttgaggttg
      181 actattaacc atagccatat tgcatgttgg gggactgatc ctgccattaa tcgttaatgt
      241 ggcagtctct gaagagaacg cgggagaagc caatccatag gcaagaaaaa taattcctga
      301 cagccaatac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggatagaatg
      361 agtgcataac aaactatagc tgtacatcca ctacacagct acgaaggatg ataatgaagc
      421 attgcctgta cgatcaatcg actttgtaga gtttcggacg aaggtccgca gaatattcgc
      481 agtattaaat aagtgttcat taatgcggca aaaacaaata caccgcatca atgtaacatc
      541 tctgcggtgt atgaagaata accagaatgg ttaataggcg ttagaccgcg cggaaggaaa
      601 tctcgcctgg aataacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct
      661 ggcggtagat agcggtaaat tcgctctccg gacggctgat aaccgttggc gtgcctttat
      721 cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgt gtgtggtatt
      781 tttcagccag tttctctgca ccaccggtac caaaaatcgg ttcatggtga ccacagttac
      841 tgcaaatatg cacactcatg ttctcgacaa taccgagcac cggaacctcg actttctcga
      901 acatcacaat gccttttttc gcatcgatca acgcgatgtc ctgcggggtg gtgaccacga
      961 ctgcaccagt tactggaatg ttttgcgcca gcgtcaattg aatgtcaccg gtgcctggcg
     1021 gcatatcaag caccagatag tcgagatccg gccacaaggt ttcctgcaac atctgcatta
     1081 acgccttgct cgccatcggt ccacgccaca ccatcgcatt gtcgtcagtg accagataac
     1141 caattgaatt ggttgccagg ccatgagaca taatcggtgc catatgggta ccgtccggcg
     1201 aagttggacg ctgattttct gcgcccagca tggtgggaat tgatgggccg tagatatcgg
     1261 cgtccagaat gccgactttc gccccttccg ccgccagtgc cagcgccagg tttaccgccg
     1321 tggaggattt acccacgccg cctttaccgg aactgaccgc aatgatgttc ttcacgccat
     1381 taatacccgg ctgattcttc acgcgtttta aggtggcgat attgtgcgac agtttccagt
     1441 cgatagcttt cgccccggtg atacgcagca gttcggcgct acattgctct tttaactctt
     1501 cgaacgcgct atgccagacg aatggcataa ccagttccac atgcaacgtg tcgtccatcc
     1561 aggcgacatg gtgcaacgct ttcagcgtgg tgaggttatg cttcagggtt gggtgctgaa
     1621 aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt
     1681 gttcgttcat cccgactcct tttttgtata gataaaccat cagctgatag tttacctgaa
     1741 gaatatagag aagtacttac ttaacatttt cccatttggt actatctaac cccttttcac
     1801 tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca
     1861 ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat
     1921 gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat
     1981 gcccacggta caccgatcat gctgaaagct cagcagcttg gtatcacccc ggagcagatg
     2041 attggcgaaa tgagtcagga gcatcagact gatttcgcag gctttaacat cagctatgac
     2101 aactatcact cgacgcacag cgaagagaac cgccagttgt ctgagcttat ctatactcgc
     2161 ttgaaagaaa acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa
     2221 ggcatgttcc tgccggaccg ttttgtgaaa ggcacctgcc cgaaatgtaa atccccggat
     2281 caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga actgatcgag
     2341 ccgaaatcgg tggtttctgg cgctaccccg gtaatgcgtg attctgaaca cttcttcttt
     2401 gatctgccct ctttcagcga aatgttgcag gcatggaccc gcagcggtgc gttgcaggag
     2461 caggtggcaa ataaaatgca ggagtggttt gaatctggcc tgcaacagtg ggatatctcc
     2521 cgcgacgcgc cgtacttcgg ttttgaaatt ccgaacgcgc cgggtaaata tttctacgtc
     2581 tggctagatg cgccgattgg ctatatgggt tctttcaaga atctgtgcga caagcgcggc
     2641 gacagcgtaa gcttcgatga atactggaag aaagactcca ccgccgagct gtaccacttc
     2701 atcggtaaag atattgttta cttccacagt ctgttctggc cagcaatgct ggaaggcagc
     2761 aacttccgca agccgaccaa cctgtttgtt catggctatg tgacggtgaa cggcgcgaag
     2821 atgtctaagt ctcgcggtac ctttattaaa gccagcacct ggctgaatca ttttgacgcc
     2881 gacagcctgc gttactacta cactgcgaaa ctctcttcgc gcattgatga tatcgatctc
     2941 aacctggaag atttcgttca gcgcgtgaat gccgatatcg ttaacaaagt ggttaacctg
     3001 gcctctcgta atgcgggctt tatcaacaag cgttttgacg gcgtgctggc aagcgaactg
     3061 gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg
     3121 gaaagccgcg aatttggtaa agcagtgcgc gaaatcatgg cgctggctga tctggctaac
     3181 cgctatgtcg atgaacaggc tccgtgggtg gtggcgaaac aggaaggccg cgatgctgac
     3241 ctgcaggcaa tttgctcgat gggcataaac ctgttccgcg tgctgatgac ttacctgaag
     3301 ccggtactgc cgaaactgac cgagcgcgca gaagcattcc taaatacgga actgacctgg
     3361 gatggtatcc agcaaccgct gctgggccac aaagtgaatc cgttcaaagc gctgtataac
     3421 cgcatcgata tgaagcaggt tgaagcactg gtggaagcgt ctaaagaaga agtgaaagca
     3481 gctgccgcgc cagttactgg cccactggca gatgatccga ttcaggaaac catcaccttt
     3541 gacgacttcg ccaaagttga cctgcgcgtg gcgctgattg aaaacgcaga gtttgttgaa
     3601 ggttctgaca aactgctacg cctgacgctg gatctcggcg gtgaaaaacg caatgtcttc
     3661 tccggcattc gttctgctta cccagatccg caggcactga ttggtcgtca caccattatg
     3721 gtggctaacc tggcaccacg taaaatgcgc ttcggtatct ctgaaggcat ggtgatggct
     3781 gccggtcctg gcgggaaaga tattttcctg ctaagcccgg atgccggtgc taaaccgggt
     3841 catcaggtga aataatctcc tttcaaggcg ctgtatcgac agcgcctttt ctttataaat
     3901 tcctaaagtt gttttcttgc gattttgtct ctctctaacc cgcataaata ctggtagcat
     3961 ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc tttcaggacg
     4021 aaaaattaca taaattctgg gcggtggagc aacagggaaa cgagttgcat atcagttggg
     4081 gaaaagttgg caccaacggg caaaaccagg taaaaagctt tgcggatgct gcggcagcgg
     4141 caaaagcgga gcttaagctg atcgcagaga aaacgaaaaa aggctatgtg gaaaatgctt
     4201 cagcaaacgt gcatatcccc cccatcacca aagccactcc tgaagttgaa acctcccctg
     4261 agagtaaaaa ccaacgccca tggctggctg atgatgcagt cattccggtc actgacgata
     4321 ttaatcgatt tgcttttcct catcgctctc gccccagaga gatcaattat ctgtacaaag
     4381 atggccaaat atggaagcgc atcgcgaata acaccagggc atatgatccc gacaacaatt
     4441 accgctctta tccagaaaac tggcagcagg cttttgctga gttacaaatg cgagttaagg
     4501 gtaatcaacc gacagggagt gcgcaatctg atgcagcatt gctctggggc ttttggaact
     4561 cttactctca agatgaactg gtcgatgact tagtcattcg ctgtggtctg gaaagtgccg
     4621 ttgaaatcgc cctttttgcc cttcaactca aatataaacc agtaaaaggc gcagtaacca
     4681 ccatcattcc acctaatcac gaagcagaat cgctaccgag ttggcatcaa cgtctatgtc
     4741 atcatctttc actcgcttca gaagatgagt ggcaacgctg tgtagataaa gtactcgctg
     4801 ctattccctc gctatcccca gcacgacaac cttttgcggc gttactgctc cctgaacgcc
     4861 cggatattgc caatgctatg gcgctacgtt atgcagatca aaacgttccg gcgatgacct
     4921 ggttaagact ggtggtaacc gatgacgccg ctctgactac agcagaaaaa tacgacttcc
     4981 caccgttgta tcatgatttt cgcgcttatc tggcaatgtt gctggcaaat aatggggtgc
     5041 gcggtgtaag ccggatactt cttgagttta cggaagagca ttctgacaat cccacttatc
     5101 tcttcgagcg tatcagcgag actgaagatc ttgtcaaatg gctatggaaa acgaatcacc
     5161 cggatgcgat tcaaattctg atcctcggtg taattggcaa gaaaaagcac ctggaatatt
     5221 taagcaaagc ctgtcaaaaa catcccgctg cggctcttgc cgcttatgct actttgctgg
     5281 caatacatga agacaaagag tggcgtaaag cgcttgtcaa actgattacc gccacgccag
     5341 ggttagtcac tgacgtcatt cccagggtta atgctaaagc ggcaggaatt ttatctgaat
     5401 gtcgtactca gttagtcgct gaagaatgtg aatacgccac tgcggatatg ttgccggagc
     5461 tgtttgtcgc tccaccgtgg gtaataaata agaaaacgaa tgtaataccc gtatttgatt
     5521 taccggtgct ttccatccct gccgtcacgg atatcacgcc aggaattaca gagttaatca
     5581 gtcatactga catatccaga ttcagtgaaa tcgcgcaata tcagtcatca cagcaaacct
     5641 tatttaccga cctgccaccg ataaaaaaag aacgctggga aacgtctttc atccctttaa
     5701 cgccagaaca gcaaatcctt tggcgactgg ggtttaagga atggcgcaga accggagaag
     5761 aacaatacga aaaaaaaata atgccacaaa gcgcagttga tgccctgtta cgtttcgatt
     5821 tccccgcgct gaaagccgag tttgcgcaat accataacaa aggaagcaga cactggcagt
     5881 tgtacgcgct ctgcttccta cccactcagc atgctatttc ttttctcaat caaataatta
     5941 atgaagagca attctccggc gagagagaaa ttctggcgat ttttggtgat gcggccattc
     6001 ctgcgtttat gaaatgtctg caacgtaagc cacaacagtt atggatattc accctcttcc
     6061 tgggcgtcag cgaattagca ttaccgatgg cgcagcgatt acagaaaaaa atgtctgctg
     6121 aagatgctcg aaaatggctg gctaacttcc cacgccatgc tgccgccggg ctacttcctg
     6181 tggccttggg caaaaaagga aaagatcgtg attgtgcccg ccaggcgctt cgtctgctgg
     6241 tgaatttaaa ccagcgagaa acgattgaag aaatcgccca ggggtataac caacctgatg
     6301 ttttagctgc gctggcaaca ttatttaata gcgagccgct ggaagaatat cccgctaaaa
     6361 tcgccccact gcccggcttt tatcagttca ccttatggcg cagaccacgg cttaaaagta
     6421 acaacctgcc tctgtcagat gacgctatgc gccacctcgg cactatgctg agttttcctc
     6481 gcgacattac cgcctacgct gggctggata tcattagaga aatcttcacc cgcgaatcac
     6541 tggctgaatt tggctgggat ctgtataccg cctggacaga agctggcgca cccgcaaaag
     6601 aaaactgggc atttacttcg cttgggattt taggcaatga cgacaccgcg cgtaaattaa
     6661 ccccgcttat ccgcgcctgg cctggggaat cccagcataa acgggcggtg tccgggctgg
     6721 atgtattagc tgatattggc agcgatgttg cgctaatgct gcttaatggc atcgcgcgaa
     6781 aaattaaatt caaagcatta caggaacatg cccgcgagaa aatcaacata gttgctgaaa
     6841 accgtgggct gactatggct gaaatggaag accgcctggc tccagattta gggcttgata
     6901 gcagtggctc gctgatactg gatttcggcc cacgtcaatt taccgttggt tttgacgaaa
     6961 ctttaaaacc tgtagtgcgc gacgcaaacg gcaaagtcct gaaagatttg cccaaaccaa
     7021 accagagcga tgaaaaaact caggcaactg acgcggttaa tctcttcaaa cagttgaaaa
     7081 aagatgtacg cgccatagcc agccagcaga ttgatcgtct ggaacaggct atgtgccagc
     7141 gcagacgctg gacggcagag cagttccgcc tgtttctggt ggagcatccg ctggtacgtc
     7201 acttaacccg acggctgtta tggggggttt ataacgatga aaacgccctt atcacctgtt
     7261 ttcgcgtggc agaagacagc acttacagcg atgcgcagga tgagttattc acgctgccag
     7321 caggaaacat cggtataccg catgtgctgg aaatcccccc tgaatccgcc gctgcattca
     7381 ggcaaattta cgttgattac gaactgcttc cccctttcca acagctcgaa cgtggtagtt
     7441 accaccttgc tgataacgaa cgtaatgtcc atgaattgtc acgctggaac ggacgtctct
     7501 gccaggccgg acgcattgtc gggcttgaac gcagaggctg gcaacgtctg gaagaaagcg
     7561 gcagcgtcta tgcgatgcgc aaatctaccc cttatggtgc tctcgaactt gaaacagaac
     7621 ctttttcatt aatttatggc gaaacggggt atagcgacct gctgcctgtc gaaagcgtaa
     7681 aaatcaccgc cccttatgat cgctatggta aacaatcctc acccactttc tccgtgctcg
     7741 acgacatcac cgccagcgag ctgattaacg acattgaatc actgtttgat taaaaggatt
     7801 tagcaatgaa aacctatatt tatcaggacg aaaaatcaca taaattctgg gcggtggagc
     7861 agcagagaaa cgagctgcat atcaactggg gcaaagtcgg cactaacggg caaagtcagg
     7921 taaaaagctt tgctgatgct gcggcggcag aaaaagcgga actgaagctg attgcggaga
     7981 aggtgaagaa ggggtatgtg gagcaagtag acgctaacgc tcactcacat gatgtctcag
     8041 caaagaaagt cctggtcaca gaggaaaaag cctcaataaa tcaccaggca gcaactaatg
     8101 gtttaccctg gctggcagac aacgatccca ttattcttcc cccgaatatt gcccgccatg
     8161 ccctcagcca ccgcctttgg ccgggagagc cagtaaaaaa accaaccaaa cccgagaaac
     8221 aaaatttaat ttcccgattg gcaaatcgta cttatcatta ttataaaaat ctgactttcg
     8281 attacagctc atgctctgat gaatgggctg atgcgattag ccaggcaatc agcttaatta
     8341 aggcccaaaa gactgagata cccgcgcgta ctatggcggt acttactttt ctggaaatat
     8401 gtttttgtca gggctggaat gatgaaacct catccgagtt gctggatgaa attgtaagca
     8461 ccttcggtat cgaatacgca actgaaactg tcatcttctg gtggcaaatt gatttcgatt
     8521 ttgactataa agatgaacat ttgactttct ttattaatta tgcagatagc agcaaggaca
     8581 gttatagaag gaataacgac aaatttagtt tacgtctgcg taaacattta tcactagcag
     8641 aagaagaagt atggcaaaat tgcattgcaa aattactcgt tgcgttacca gatatatcac
     8701 tctatcgcca accgttaatt gccattttaa tgccagaaat acccgaagtg gcccatgaga
     8761 tcgtacatcg tttacaccag gtcgcagatg ttccacaact tgaaatgttg aaactggtag
     8821 cgacagatcc attcacgctc gaaatactgg agaactatca atacattgat gtatttaaca
     8881 cctatggtgc cagttggtca gctacggtat tacgggaaca aggtatagcg gcactggctc
     8941 gcctagcttc ctatgctgaa gaagataatt gcggtgacgt actgaaatgt attaatcatc
     9001 cgttggccat tacggcactc atcgaagccg ccgatactaa taaacgctgt tatgaacgac
     9061 taataaaaag tgccgaaaat tttccttctg ccacgatagc ggcattagcc gaagcattgg
     9121 taaaaaaaga tgataaacgc aaacatacgt tgctggtttc attacttaat gcaaatcctt
     9181 tattggtcga aaaattagtc atcagattat cgtcgcaagc ggcaaattta ttgcaacaat
     9241 gtcagcaatt gctgaatagt cgcaatgaag attttgcaaa ctcagaaatt ctaccaatgg
     9301 tactagtctc gccgccctgg gaaactcaaa ataaaaataa cgacaacagt cctgtgggta
     9361 aatacccggc gaaaatcccc cctttaccgt tgttttatca acctccatta tggactcgcc
     9421 caaaactcaa aaatagcagc aaggtactgc cagataacgc cctcctccac ctcggtgaaa
     9481 tgctccgctt ccctcaggaa gaggcgctgt atccgggatt attgcaggtg aaagatgcct
     9541 gtaccgccga ctcactggct gaatttgcct gggatctgtt taccgcctgg ctgactacgg
     9601 gtgcaccatc gaaagaaagc tgggcgttca ctgcgttagg tgttctcggt aacgacgaca
     9661 ccgctcgcaa actgacgcca ttaatacgcg cctggcctgg cgaatctcag cataaacgcg
     9721 ccaccgttgg gttggatatt ctcgccgcta tcggtagtga tatcgctctg atgcagctta
     9781 acggcattgc tcagaaactg aaattcaaag ctttacagga gcgggcgaaa gagaaaattg
     9841 ccgacattgc tgaaagtcgc gaactgacag tggaggagct tgaagatcgg ttagcaccgg
     9901 atctcagtct ggatgataac ggttcgttgc tgctggattt cggcccacgc cagttcaccg
     9961 tcagctttga tgaaacctta aaaccgtttg tgcgtgatgt ttctggcagc cgtctgaaag
    10021 acctgcccaa accaaacaaa agcgatgatg aatcacaggc gaacgatgct gttaaccgct
    10081 acaaactgct gaaaaaagat gcgcgtaccg tcgccgccca gcaggtggca aggcttgaat
    10141 ccgccatgtg cctgcgccgc cgctggtcgc cggaaaactt ccaactcttc ctggttgtgc
    10201 atccgctggt tcgccactta acccgccgtc tgatttgggg cgtttatagc gccgaaaacc
    10261 agctgcaggc ttgctttcgc gtagcggaag ataacagcta cagcaccgct gacgatgatc
    10321 ttttcaccct gccggaaggc gatatctcta tcggcattcc tcacgttctg gaaatatcac
    10381 caacggatgc tgccgccttt ggtcagcttt ttgccgacta cgaactgcta ccaccgttcc
    10441 gtcagcttca ccgtaacagt tacgccctga cagaagccga gcgcaacgcc agcgaactga
    10501 cccgctgggc aggcagaaaa tgcccgagcg gtcgggtaat ggggctggcg aataaaggct
    10561 ggataaaggg cgaaccacag gatggaggct ggatcggctg gatgatcaaa cctctgggcc
    10621 gctggtcgtt aatcatggaa atcgatgaag gctttgcggt aggcatgtcg ccagccgaac
    10681 tgagtgccga gcaactctta agcaagctgt ggctatggga aggcaaagcg gaaagctatg
    10741 gctgggggag taattcaaca caggaagcgc agctctccgt actcgatgcc atcaccgcca
    10801 gcgagctaat taacgatatt gaagccctgt ttgaataagg aaagagcatg gacaaggaat
    10861 taccgtggct ggctgataac gcccaactgg aactgaaata taaaaagggt aaaacaccac
    10921 tcagccatcg caattggccg ggcgaaccag tgcctgttat cactgaaagc atcattcaga
    10981 cactgggtga tgaattgcta caaaaagctg agaagaaaaa aaacattgtc tggcgttatg
    11041 aaaatttttc actggagtgg cagtccgcca tcacgcaggc catcaacttg atcggcgaac
    11101 acaaaccctc aatcccggcc cggacaatgg cggcgctagc ctgtatcgcg caaaatgaca
    11161 gccaacagtt gctcgacgaa atcgtccaac aagaggggct ggaatatgcg acggatgtag
    11221 tgatggcgcg tcagtgcatt gcgcggcgtt atgagagtga ttcactggtc gtcactttgc
    11281 agtatcagga cgaggactat ggctacggtt atggctccgc gacctataac gatttcgatc
    11341 tccgtttgcg taagcatctg tcgctggcag aggaaagttg ctggcagcgt tgtgccgata
    11401 aactcattgc cgcactgcca ggaataccca aaattcgccg tccttttatt gcgctgatcc
    11461 tcccggaaaa gcctgaaata gccaatgaac tcgccagtct tgaaagctcc cggtcaagtc
    11521 tccattcaaa ggagtggtta aaggtcgttg ctactgataa tacggcggta aaaaaactcg
    11581 aacgatactg gggcctggac gtatttagcg atcgcgaagc cagctacatg tcacaaaaaa
    11641 accacttcgg ctacgcggcc tgcgccgccc ttttgcgcga acaaggactg gcagccattc
    11701 cgcgcctcgc gatgtatgcc cataaagaag attgcggcag tctgttggta caaattaacc
    11761 atccgcaggt catccacacc ttgctactgg tggctgataa aaacaaaccc agcctgcaac
    11821 gtgtggctaa atacagtaaa aacttccccc acgcgacgct cgcggcactg gcagaactgc
    11881 tggcgttaaa agaaccacca gcccgccctg gttatccaat catcgaagac aaaaagctgc
    11941 ctgcccagca aaaagcacgc gatgaatact ggcgtacgct gttacaaacg ctgatggcat
    12001 cgcagccaca actggcagaa gaggtgatgc agtggttaag tactcaagcc cgggcagtac
    12061 tgaatagtta tttattggca ccgcccaaac cggttattga tggcaccgat aacagcaatc
    12121 tgcctgaaat cctcgtttca ccaccgtggc gtagtaagaa aaaaatgaca gctccacgtc
    12181 ttgatttggc accgctcgaa ttaaccccgc aagtttactg gcaaccaggc gaacaagagt
    12241 ggcttgcctc cactgagtct gcccgttatt tcagcacgga atctcttgcg gaacgcatgg
    12301 aacaaaaaag tgggcgagtt gtattacagg aactgggttt tggggatgat gtatggctgt
    12361 ttctgaatta tatactcccc ggaaaactgg atgctgcacg caattcactc attgttcagt
    12421 ggcattacta ccaggggcgg gttgaagaga tcctgaatgg ctggaactcc ccggaagcac
    12481 aattagcaga acaggcgctc cgcagcggtc acatagaagc gttaattaac atatgggaaa
    12541 atgacaacta ctcacgttat cgtccggaaa agagtgtctg gaacctgtat ttattggcac
    12601 agttgccgcg tgagatggct ttgaccttct ggctgcgtat caatgagaaa aaacatctgt
    12661 tcgcgggtga ggactatttt ctcagtatcc tcgggttgga tgcgctacca ggtctgatgt
    12721 tggctttttt acatcgtcca aaagaaacat ttccgttaat tttaaatttt ggcgcaacag
    12781 aactggccct gcccgttgcc cgcgtctggc gacgtttcgc ggtgcagcgt gatctggctc
    12841 gccagtggat tttacattgg ccggaacata cggctactgc acttatccct cttgtcttca
    12901 ccaaatccag cgataatagc gaagctgcat tacttgccct gcgtttactt tacgaacagg
    12961 gacatggtga attgctgcaa accgtggcaa accgctggca gcgtacagat gtatggcctg
    13021 ccctggagca tttactgaaa cagggtccaa tggacattta cccggcacgc attccaaaag
    13081 cccctgattt ctggcatccg gcaatgtggt ccaggccgcg acttatcact aataatcagc
    13141 ctgttaccga tgacgctctg gaaattatcg gcgaaatgct gcgctttacc cagggcggac
    13201 gtttttatag cggtctggaa caactgaaaa cgttttgcca gccacaaacg ctggccgctt
    13261 ttgcctggga tctcttcact gcgtggcaac aagctggtgc ccccgcaaaa gacaactggg
    13321 catttctggc gttaagtctc tttggtgacg aaagcacggc tcgggatctg acgacacaga
    13381 tcctcgcctg gccacaagaa ggcaaatctg cccgtgctgt cattggtctg aatatactta
    13441 ccttgatgaa taatgatatg gcgctgatac agctgcatca tgtatcacaa cgggcgaaat
    13501 cctcttcatt acgtgaaaac gcagcggaat ttcttcaggt ggtcgcagaa aatcgcgggc
    13561 taagccagga agagttagcg gacagattag tcccaacact gggtcttgat gatccgcagg
    13621 cgttgatttt tgattttggt ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc
    13681 cggttatctt tgatcaacaa aacgttcgcc agaaaagtgt tccccggttg cgtgccgatg
    13741 acgatcaact gaaagcgccc gaggcactgg cccgactaaa agggctaaaa aaagatgcta
    13801 ctcaggtgag caaaaacctg ctcccgcgtc ttgaagctgc cctacgtacc acccgacgct
    13861 ggtcgctggc agattttcat tctctgtttg ttaatcatcc ctttacccgc ctggttaccc
    13921 agcgcttaat atggggggtt tatccggcaa atgaaccgcg tcgtttactc aacgcctttc
    13981 gtgtggccgc agagggggag ttctgcaatg cgcaggatga gcccattgac cttcctgcgg
    14041 atgctctgat tggcattgcc cacccgttag aaatgacagc agaaatgcgc agtgaatttg
    14101 cacagctttt tgccgattac gaaattatgc cgccttttcg ccagttgtcg cgccgcacgg
    14161 tgctgctcac acctgacgag tcaaccagta acagcctgac tcgctgggaa ggtaaatccg
    14221 ctaccgttgg gcaacttatg ggaatgcgat acaaaggctg ggagtcaggc tatgaggacg
    14281 catttgtcta tgacctgggt gagtaccggc tggtccttaa gttttcaccc ggttttaacc
    14341 actacaatgt tgatagcaaa gcgctaatga gcttccgttc tcttcgagtg taccgtgaca
    14401 ataaatccgt cacttttgcc gaacttgatg tgtttgattt gagtgaggcg ttaagcgcac
    14461 ctgacgtcat tttccattaa aacacagcgg gcagtgatgc gactgcccgt tatctacacg
    14521 acttaccagc ggggaaagcg atgatcgtgc aaaaagagct agttgctatt tacgattatg
    14581 aggtccctgt acctgaagat ccgttttcct tcagacttga gatccataaa tgctctgaat
    14641 tatttacagg ttccgtctat cgactggagc gattccggct acatccaaca tttcatcaac
    14701 gtgatcgaga agatgctgac ccgctaataa atgatgcgtt gatttatata agagatgagt
    14761 gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca atttttaatc
    14821 gtgaactaca gaatatattc aaccaagaaa tagaataata tactctagat aattaaagtc
    14881 gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga aacggtattc
    14941 ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc
    15001 cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg tgtccatccg
    15061 ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg ttgatactgg
    15121 tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg cggcaccacc
    15181 cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa cgggcaggat
    15241 tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt tattcgttgg
    15301 tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag aacctgcctc
    15361 accagcaggt ttttttattt tctgcgatct actttccaga tattttttgc caatagccca
    15421 acataaatat tcacatcccg gtcaaatggt gcgttgaata taaaatcgat gaatttataa
    15481 ataaaaatga gttatatgat gaattaataa aaacagtaat cattttatta aagggggaag
    15541 cggttttccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc aatcaaccgt
    15601 aacagagcga ctaatagctc cagtacagca atgagcacgg ttaacttttg tgtcagcgtg
    15661 tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac actgcctgtc
    15721 gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc accacctgca
    15781 gcaccccccg tccggtagcc aggacttgaa ataggttcga tccggcgcaa cgtcccaaca
    15841 gtcaggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg ttataattcg
    15901 ttcgttggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaaatag taaataaaag
    15961 aacctgcctc accagcaggt ttttttattt actgtgatct gctttccaga tatttttcgc
    16021 tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt tcattaatac
    16081 atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg cctgctgctg
    16141 tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgccccc tgcccgcccg
    16201 gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc gcgcaaaata
    16261 ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc actcttgcca
    16321 ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc ctgtctgaat
    16381 tactggcaac cgcaattagc ggcgatgccg ggttaaccat ccagggcggc gcatctacca
    16441 ccgaagatca aatcaagtac ggctggaact acgccctgct tatcaaccat ggtccgtcaa
    16501 cggaagcgct ggtccctgcc ccgctctacc agggaatgcg cgacggcaaa atcgtccgtt
    16561 ttgaagagat aacgcgtaca ccactggaag tgcaggactg tctgttaggt atgctttctg
    16621 acagggtgat gacggtgccg gaactcaccg gtgaagccag tcaactctat gcgcgggaag
    16681 gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgaa atgagcgctg
    16741 cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc gcccaggagc
    16801 tggagcttgt cgccagtgct tcggcgcgtt tgctggccca tagcggtatt ccgcataaag
    16861 tgcctgacgc ggtactggag ttactggtcc gcactttccg cgatctgcgt gccaacggcg
    16921 aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa gccgtgaacg
    16981 tggcccacgc tgtgggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt gagccagccg
    17041 acctggtgga ctgtattgcc ggaaccatcg tcaaagataa cgaagaagat cgcgcgcgtc
    17101 tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga agctcactgg caggcttatt
    17161 atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt aattgtcggc
    17221 atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga aagccagcgg
    17281 ccacgatacg tgttgattga aggcccggct gattttaatg accgggtaga cgaactgttt
    17341 ttagcccacc agcttccggt agctatttac agttattgcc agtatcagga cggtgcagcc
    17401 cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca ggcgctacaa
    17461 gccgctcgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg ggcacagagt
    17521 gaagaagaga acgattcgcc tgatacgcaa gaggaaagcc agaccttgct gctgcgtgcc
    17581 acccgcatgg ataacagcga taccctgtgg gatcacttgt tcgaagatga aagccagcaa
    17641 actgcattac cctctgcact ggcgcactat tttgcccaac tgcggggcga tttccccggc
    17701 gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg ggcggtgcag
    17761 caaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctccggc actggcaaaa
    17821 atgtggcgcg aatgcccgca ggacattaac aagccagaat tgccctcgct ggcagatgcc
    17881 gttacaggtt gttatctcac accctacagt gaaaagcgcc ttgatgtgct ggcaggatac
    17941 ctttcaggaa tgcctgcccc ggtctggcaa agctggtgct ggaagtgggg cttacagcag
    18001 gcgggagaac aactgctaaa aacggttctt acccgtttgc gccaacacca cttgcctgct
    18061 tcgaccgcgg atatggctgc cgctcatctg cattcgatgg cgctggcaca gttgcgcgga
    18121 cataaactac cgttacgcac tgactggctg gatgccatag caggctcgct gattaaagaa
    18181 gccctgaatg cgccgttgcc gtggagctat cgcggcgtta ttcatcccga caccgatccg
    18241 attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact tgcccctttt
    18301 acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac cgcaatctct
    18361 cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca aagtcaggtg
    18421 ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg aagcacactg
    18481 acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag ccagcatgcg
    18541 gcattgattg aggccgcctg ctttggtgca acactccagg aagccgcacg ccataaatta
    18601 gaagccgata tgctggacgc gggtggaatc ggcagtatca ccacatgtct tagccaggcg
    18661 gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac attattaatc
    18721 gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata tgccttatgg
    18781 cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac gttatgcgcg
    18841 gctatcgatc gcacgctgtg gctgtgtgaa tctaacggca gaccagatga aaaggagttt
    18901 cacgctcacc tgcatagctg gcaggcgctt tgccatatcc tgcgcgatct acatagcggc
    18961 gttaatttac ccggcgtttc tctttctgcg gcggtagcct tactggagcg acgcagtcag
    19021 gcaatccatg ccccggcgct ggatcgcggc gcggctcttg gcgcactgat gcgtctggaa
    19081 catcccaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc cccggcacaa
    19141 tccggcgaag cgctgcacgg tttgctagca ttagcccgtc atcaactggc ctgtcaacct
    19201 gcatttatcg ccggtttcag cagtcattta aatcaactaa gtgatgccga ttttattaac
    19261 gccctgcccg atttacgcgc ggcgatggcc tggctaccac cacgagaacg cgggacgctg
    19321 gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc gctgcaaatg
    19381 ccgttgcatt gtccaccaca agccattgca catcatcaac aactcgaaca gcaggcactg
    19441 gcatcgctgc aaaactgggg agttttccat gtctgaactg aacgatctcc tgaccacccg
    19501 tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc tttgtgggct
    19561 ggatgacaac gcccggcaga tagaccacgc gctggagtgg ctgtatgggc gcgatcctga
    19621 acggctccag cgtggtgaac gttccggtgg attaggtagc tcaaatctca ccccccctga
    19681 gtggatcaac agtattcaca cgctgtttcc gcaacaggtg attgagcggc tggaaagcga
    19741 tgccgtactg cgctatggca ttgaagatgt ggtgacgaat ctcgacgtgc tggaacgtat
    19801 gcagccttct gaaagcctgc tacgcgccgt tttgcacacc aaacatctga tgaatcccga
    19861 agtactggct gccgcccgcc ggatagtgcg ccaggttgtt gaagaaatta tggctcgact
    19921 ggcaaaggaa gttcgtcagg ctttttctgg tgtccgtgat cgccgtcgcc gctcatttat
    19981 tccactggcg cgaaactttg atttcaaaag tactcttcgc gccaacctgc aacactggca
    20041 cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca ttaaacgcca
    20101 aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggctcgatgg tcgattcggt
    20161 gatccactct gcggtgatgg cggcctgttt atggcagtta cccggcattc gtacccatct
    20221 ggtggcgttt gacaccagcg tggttgatct cacggcagac gttgccgatc cggtagagtt
    20281 attaatgaaa gtgcaattag gcggcggaac caatatcgcc agtgccgtgg aatatggtcg
    20341 gcaacttatt gaacaaccag cgaaaagcgt cattatcctc gtgagtgatt tttatgaagg
    20401 gggttcatca tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg gcatcaaagt
    20461 gctggggctg gcagcgctcg acagcaccgc aacgccttgc tatgaccgcg atatggccca
    20521 ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca ccgggcgaac tggcatcatg
    20581 gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga actgacaccg
    20641 caggccctga cggcgttaag caatgccggt tttgttaagc gcagccttaa ggaactggaa
    20701 aatggcaacg tcccggaaat cagccatgag aacggcgctt taatcgccac cttcagtgac
    20761 ggtgtccgta cccagctggc gaacggtcag gcactgaaag aggctcagtg cacctgcggg
    20821 gctagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcagcg actttgtgcc
    20881 accacccggc ccacggaaaa agaagaagag tggcagcctg caatctggct ggaagaactg
    20941 gctaccctgc ccgatgccac ccgcaaacgt gcacaggtgc tggtcgctaa aggcatcacc
    21001 attgagttgt tctgtgcgcc tggcgaaatt ccctcagccc gcttaccgat gagtgatgtg
    21061 cgtttttatt cccgcagcag cattcgcttc gcccgctgtg attgtattga aggcacactt
    21121 tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaaactca gcaagcggaa
    21181 tttactcatt taatctggca gatgcgcagc gaacacgtca catcatctga cgatccgttt
    21241 gccagcgaag agggcaacgc gtgtcgtcaa tatgttcagc aactaagcca ggcattatgg
    21301 ctgggcggca tcagccagcc gcttattcac tacgaggccg ctttcagccg cgcgcagcaa
    21361 gcggcggaac gctgcaactg gcgctgggtg agtgaatcac tacggcaact gcgcgccagc
    21421 gttgatgcct tccacgcccg cgccagccac tatcatgcag gagaatgctt acgtcagctt
    21481 gcggcattaa acagtcgatt aaattgcgca caagagatgg cgcggcgcga cagtatcggt
    21541 gaagttcctc ctgtgccgtg gcgcacggtc gttggcgctg gcattgccgg agaagcaaag
    21601 cttgatcatc tgcgactggt gtctttaggt atgcgttgct ggcaggatat tgagcagtat
    21661 ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcacct ttcgcgcagt
    21721 tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt tagttttcag
    21781 gctggcgcac tggcgggcgg gcaaattgtt tcacaagcag caaaacgcag tgccgatggc
    21841 gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc gcctgatgcc
    21901 tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg ggaatattta
    21961 cgccagcgtc cccccgcctg catacggcct cttaatcagg tcgataactt atttattctg
    22021 ccggtcgctg agtgtatttc tctcggttgg gatagcagcc gccagacgct ggatgcgcag
    22081 gtaatcagcg gcgaaggtga agataatctg ctgacgttat cactgccagc ctcagccagc
    22141 gcgccttatg ccgttgaacg catggcggcg cttttgcaac aaacagacga ccccgtgtgt
    22201 ctggtttctg gctttgtcag ttttgttgat gggcaattga cactggaacc acaggtaatg
    22261 atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg cgcctgtggt gacatcacta
    22321 ccttctgtca gcgttctgcc tgtgccgtct actgctcatc agttgctgat gcgctgccag
    22381 gcattactta ttcaactgct ccataacggc tggcgctatc aggaacagag tgctattggt
    22441 caggcagagt tgctggcgaa tgacctcacc gcggtgggtt tttatcggct ggcacatgtg
    22501 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
    22561 ttgctttgcg aacaattatt ccccatactt cagcaacaag gatgaaatag tgctttttac
    22621 taagagttct actccagttc cggactgctc acgccacagt attaggcata tcctatatag
    22681 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
    22741 caataagctg ttttcccttg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
    22801 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
    22861 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttca gaaaccaaaa ttcaatttgc
    22921 ctctattggt gcaaccacca aagaagacgc tgccaagaca cttgagccgt taagcgccaa
    22981 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
    23041 aaacgtgacc atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
    23101 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
    23161 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
    23221 ccatacccgt cttagctttt ctccacatgc tggcgcgctg ccagcccacg cagaaaataa
    23281 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
    23341 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
    23401 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
    23461 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
    23521 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
    23581 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag tatacgcacc
    23641 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
    23701 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
    23761 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
    23821 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
    23881 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
    23941 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
    24001 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
    24061 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgttcctg
    24121 acgcaaacgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagatagtc
    24181 aaaggcatgt tcttcaaaag ctttaatagc gtattcgtca aacgcggtga gaaaaacaat
    24241 atacggacga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
    24301 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
    24361 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacgcgcag
    24421 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
    24481 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttatccca
    24541 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
    24601 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat taaatgttgc
    24661 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
    24721 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
    24781 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
    24841 agataagcat tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
    24901 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
    24961 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgggg gttcacctgg
    25021 gcgtgaagca gtttgatctc tgactgggtg agcatcgctt tttgccgctc atattgcccg
    25081 gcaaggatct gcgccgaaag tagttgcgca atcccctcac ccaacgtgcg gttgattgaa
    25141 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacccg ctgattttca
    25201 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
    25261 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaagta
    25321 taagttgaag aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc
    25381 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
    25441 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
    25501 gctgccactt tcagcgcagt ggcagaaaaa gctgaagtgt atttttcaaa catcgcgcgt
    25561 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
    25621 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
    25681 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
    25741 actttatcag tgcgcccgcg gcggatcagg atgctgtgta ccaggccacc gagtaatcct
    25801 tcaacgatgg tcgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
    25861 aacccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
    25921 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
    25981 cccatgatgc agaagatgga aaagacgatg tagcagagaa acttatgcgg cagacgaacc
    26041 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccatgc aatgactaaa
    26101 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
    26161 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
    26221 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
    26281 ccctataaat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
    26341 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
    26401 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtgg cagaggcgtt acggattgct
    26461 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
    26521 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
    26581 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
    26641 ttacttgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
    26701 ttaccccgcc cagacgctca ccacgcatct gtttattcct ctgcgccgac ggcttcagtg
    26761 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
    26821 cgccatctgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
    26881 gaatattcac gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggctg
    26941 gcgcattgat gtcctcgccc actcactcaa tcaactacgc cctgaactgt tcgaaggccg
    27001 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
    27061 ttggcaagaa caaggctatg atattttccc actcggcatt taatgattcg ttaacaaatg
    27121 cgctttactg tacaatcctt tcgttaacat aaggagtgca ttatgcgcat agctaaaatt
    27181 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
    27241 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
    27301 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
    27361 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
    27421 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
    27481 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacccttcg
    27541 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
    27601 gcagcacgcc gtacaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
    27661 gaacagcaat cgccagcacc gcaacgggcg gaaaagtctg cccaacggcg gcaatagttt
    27721 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtac
    27781 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
    27841 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
    27901 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
    27961 gcggatcgcg caacatcttc atacgcttac gcctccacca gcagatcctg aaaatgcagc
    28021 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
    28081 aacagggaga gcgcatcgcg cagcgtcatc tcttctgcca gtgcctcacc ttctgctcgt
    28141 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttca
    28201 ctacgtccaa aaaactggcg gacaaaatca ttcgccggac gagtcagcat cgtcagcgga
    28261 ttcccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
    28321 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
    28381 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
    28441 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcta ctcccacacg ttgctgctga
    28501 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
    28561 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
    28621 gtggcaatgt tttgtgccac gctccagtgg ggaaacaggc caatagattg aatggcatag
    28681 cccatacggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
    28741 atctctccgc tgtcatgttc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
    28801 gaaccagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
    28861 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc taaattcaat cattacgttt
    28921 caccttcagc agtgcgataa gcaaattgaa cagcgcgtcg gtaagcaccg ccagaacaat
    28981 taccgggatc acccccagca acactaaatc aatggcgctg cttaacagcc cctggaaaac
    29041 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatgc ctacagtttg
    29101 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagcggta actgaacatg
    29161 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
    29221 gatctggttc aagccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
    29281 cgcaatcagt gcgggtgtca ttccggttcc tgctatgcca agcttcccca gccacggaaa
    29341 ggccgtaacc agtccggcaa gcggcgcaat cagcaggcca aagagcgcca ccgaaggcac
    29401 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgcgcagtgg aaaagtagca
    29461 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
    29521 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
    29581 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
    29641 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cgctacaggc
    29701 cagcaatgcc agcgccgcag ccagccagaa accactgccg aggctggtac gcgccagcga
    29761 actgccattt tgcgccagtt gggtcgccgc ctttccagct ccccacacca gcaatacgaa
    29821 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
    29881 tgtcagccag gcgcaaccaa agccaaccag catccagatc gtttgcggcc acagctgcca
    29941 gagatgtcgc ccttcacccg agactaaacg attaggcgcg tagctgataa acggcagcgc
    30001 tgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
    30061 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
    30121 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
    30181 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tccttcaaca ccgattcacg
    30241 caccactggc gcaggcgcgt agataggttg cacgccttgc ggatcgctta aggtttgcag
    30301 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
    30361 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
    30421 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
    30481 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
    30541 gtcagcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
    30601 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
    30661 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
    30721 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
    30781 cccgcgcacc acaggagtcg ttccaagttg cactttattg accgtcggta cgccgtggct
    30841 ttccagtacc tgcaaaatga tattgccgag cagcgcgcct tcggtatcga tttttgaacc
    30901 gactttaacg ggggaagccg cgtgcagcgg caggctcacg gctgccaaca aaaccagtga
    30961 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
    31021 gcgaattaag agaaaagcat agttgataat ggcgggatta gcctgagcgg acggattcag
    31081 ttgcagaatc agataaatac ttaagaggca tattcggtcc ggcgtttcct gccggatgcg
    31141 gcgcgagcgc cttatccggc ctacaaaggg cgaaaattca gcaagttaag atatgaacat
    31201 caggcaaagg gcgcaaacgt cgcgcccttg ctaaagcatt acagcaactc aaactcgcct
    31261 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ctcggcgtca
    31321 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctaacggtc
    31381 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
    31441 ctcatggaag ccgtcacatc ctgcaagtac atctgcacta ccgtggcccc ttcgcgttta
    31501 ccggtgttcg tcacctgcac gctggccgtc actttgccgt cacgcttcat ggtcggcgca
    31561 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
    31621 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
    31681 tacggacgac cggtgttcag atggctgtag tacaccggga tctgcccgac agaacgcggg
    31741 aaggacatcg gcagcttgcc ggacgggttg taatcgccaa acaacacatc ggcaattgca
    31801 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgttcttct
    31861 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
    31921 ttcagcgccg caatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggaa
    31981 gcttcgtgcg ccatcccctg tgcttcaccg actacagcca ccaccacatc agattgtttc
    32041 gccgtctgca ccgcttcatc aatcatctct tgcggcgaac gcggatcgac tttgaccgcc
    32101 tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg
    32161 gcatacagca ccttaccgtt ttcaccgacg gcatttttaa tccctgttag cacagtaaca
    32221 gattgatcgg cgacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc
    32281 agtggcccaa ccaccgcaat ggttgcggat tttttcagcg gtaacgtttc gagacggttt
    32341 ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt
    32401 tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta
    32461 aacaacccca tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctctgcc
    32521 atggtcactt taccggattt aatcaaccca ggcagatact ttgagtagta ctcgtcgctc
    32581 atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc
    32641 gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag
    32701 ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg
    32761 ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac
    32821 ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta
    32881 ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga
    32941 tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga
    33001 tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg
    33061 accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt
    33121 ccgaccgttt ttactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac
    33181 accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt
    33241 aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc
    33301 gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccgggccg
    33361 acgctgatta agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg
    33421 gtgacgaacg catcccgcgc ttcaggcgtt aatggatggt taccgaataa atcatccgcc
    33481 agtgctggct gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg
    33541 atttttcttc ctttgttgcc cgacgtggca gcgaaaatgg tgcaaaaacc gtagtttgcc
    33601 ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt
    33661 cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca
    33721 aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg
    33781 cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca
    33841 agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg
    33901 aattgtggcg ggtgctgaaa gcctgcgtca ccgccgacaa aattattctg atgcaggctg
    33961 ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgattatgat cgcgatatcg
    34021 tgatcatcag caccctgcgt ctcgacaagc tgcacgttct cggcaagggc gaacaggtgc
    34081 tggcctatcc gggcaccacg ctttattcgc tggaaaaagc cctcaaaccg ctgggacgcg
    34141 aaccgcactc ggtgattgga tcatcgtgta taggcgcatc ggttatcggc ggtatctgta
    34201 ataactctgg cggctcgctg gtgcagcgtg gcccggcgta taccgaaatg tcgttattcg
    34261 cacgcataga tgaaaacggc aagctgacgt tggttaacca cctggggatt gatctgggcg
    34321 aaacgccgga gcagatcctt agcaagctgg atgacgatcg catcaaagat gacgatgtgc
    34381 gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag
    34441 ccgacacgcc cgcacgttat aacgccgatc cggatcggtt atttgaatct tctggttgcg
    34501 ccgggaagct ggccgtcttt gcagtacgtc ttgatacctt cgaagcggaa aaaaatcagc
    34561 aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc
    34621 tggctaactt cgaaaatctg ccggttgccg gggaatatat gcaccgggat atctacgata
    34681 ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga
    34741 tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct
    34801 tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt
    34861 taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg
    34921 cgggcgatgg cgttggtgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgagg
    34981 gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg
    35041 ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg
    35101 cgctggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga
    35161 tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc
    35221 aggattacat cgttaaaaaa ggcgtggatg tgcatgcgtt gaaagaacag atgctggagc
    35281 tgctacagca gcgcggcgcg caataccctg ccgaacataa cgtcggtcat ttgtataaag
    35341 caccggagac gttgcagaag ttttatcgcg agaacgatcc gaccaacagt atgaatccgg
    35401 ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg
    35461 cagagtatcg ccatccgtaa ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    35521 tttgggcggc tttttgtttt ttatagctca acgctgctgc cggaacaggc atcactttac
    35581 cggtttcaat ccaggtacgc aggcgactgg catcggcaaa atgggtatat ttgccaaacg
    35641 catccatcac taccagcgcc accggtttat tattgataac cgtacgcatc accagacaat
    35701 ggcccgccgc attggtaaag ccggttttgg ttagctggat attccagtta tcgcgataca
    35761 ccagatgatt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    35821 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    35881 tggttaagtc acgggcggtt gaaacgttat gcaccgacaa tccggttggt tcaacaaagc
    35941 gcgtattgtt cattccgagt gatttcgctt tcgcattcat cgccttaata aaggctttgt
    36001 aaccaccggg ataatggtgc gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    36061 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    36121 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    36181 catccagcac gaccatcgca gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    36241 ccgggtggtt cgaatagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    36301 aggcaatttc cggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    36361 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    36421 tgagcattca gatagtggtt cacgcgcacg ggttgcgcac cgccggagta aggatttact
    36481 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    36541 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    36601 acgataaccg tagccccata gtataacggt aagggcgagc agcacttcca gtaccagcac
    36661 gccaatcgcc agcgtcgaac tggagaagct aaggccttcc tctttgttga tattcaggaa
    36721 gctcggaata ccaaggtaaa gcaggtaacc ggtgtaaaac agtgccaccg tgccgaccag
    36781 cgcacacaac cagaccagcg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    36841 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    36901 catccaccag atgacccgcc ccatcaccgc gaccccagcg agcataacgc cataaaacag
    36961 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    37021 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    37081 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    37141 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    37201 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gctcatgtaa
    37261 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga ttcacagggt gtatgcttac
    37321 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    37381 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    37441 cgttgcggcg catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    37501 cggcatgatt ggcgaccagg tgctctatct gtgcgggcgg cggtttggcg gcaagctgtt
    37561 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaaatta tccaacgcca
    37621 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    37681 gctgattggt gccagccagc taccgccgaa aatctttctg ccgctgaata ttctcggcgc
    37741 atttgcctgg gcgttgattt ttaccactat cggttacgct ggtggtcagg tgattgcgcc
    37801 gtggttgcac aatctcgatc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    37861 tctggtggta ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggataatca
    37921 ggcgtaaaac cattgctctg gataaggcgt gcgtaccgca tccgacatcc ggtgcaccat
    37981 gcctgatgcg gcgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    38041 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacatt aagccgccat
    38101 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    38161 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggt tccgcgtcgg
    38221 gcttcacgtc gctgtcatcc atgccattca ttggcgtggc gatcgcccca ggcgcgactg
    38281 cgttcaccaa aatcttatgt ctgaccagct ccagcgccat cgctttggtt aacccaccga
    38341 gcgcatgttt agcggctgtg taggcgctgg catccggcag cggcgtatgt tcatgtaccg
    38401 acgtaatgtt gatgatgcga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    38461 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    38521 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    38581 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcaga ttgccgagat
    38641 ccagttgcac gatctccgca cgtacgccgt ggctaaccac ctcacgcgcg gtatcttttg
    38701 ccccttcttc atcagagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    38761 atgcgcactc tttgccgatc cccgaatcgg aggcggtaat tatcgcgacc tgtgccatcg
    38821 agttctccac ttaacgctga ataaacgtta agtatagaag tcacatgtca tcagcgtttg
    38881 taacccccgc ccaacgcacc agtgagttga atggaggcat ccagccactg cccttgcaat
    38941 aacaggccat tggcccgctc acgcagagcg gggattctgg cttcgctgac gcgggaacca
    39001 gcaatgatgc ccgcgttaaa gcgggcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    39061 tcaatttgcg cctgatgctg gtttttctcc gccagtgtct gaacctgact ggctgcccgc
    39121 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    39181 tcggcttttg cgatatcgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    39241 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    39301 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    39361 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    39421 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    39481 ggtttaagcg tcaatgactt agtctggtga tttgtgagtg caattaaccg tgcctcaata
    39541 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    39601 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    39661 ataatggtgt tctgctcttt ttcgatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    39721 tgccactccc agtacaggcg agctacgctg ccagccagca attggcgggt ttgctcgcgt
    39781 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    39841 cagatatcga gatgccagcc cgccgttatg ccaaaagtac cgttggtgta ccacggaccg
    39901 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaaaccttc tgccgacatt
    39961 ttttgccgct ccatatccgc agaaaagtcg atctgcggac catcctgagt agcaattgcc
    40021 ttcgcctggg cttcagccag ttgaatgcgt tgttcagcca cctgcatatc cggtgcgttc
    40081 tgtagtgcat tgttaattaa tgaagtgagt tgattatcgt gatactccag ccaccattgg
    40141 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    40201 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggt cagcatcagt
    40261 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    40321 ggtaagaaaa ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    40381 caatacatca gtagcaataa aacgacatat ccagaaaaat atacactaag tgaatgatat
    40441 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    40501 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatc
    40561 attttcctga ttgggctact ggtggtgacg ggcgtattta agatgatatt ttaaaattaa
    40621 ttaatgtcat cagatccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    40681 tatcatgtgc attgcttcat ataatcactg gtgcaaggag cgcgcagggg gcgaccaatc
    40741 gccgccgccc cctgcccaca tccatgtggg ctgccccggc cttcagggaa cgcctcggcg
    40801 attttgacgc caccaaacaa ccgtgcggcc tattgataaa gagctaacac cttgtcaaaa
    40861 aacatcacta tggtttctta gagtttctca atatcaattg cctgaatagc ccttgcaata
    40921 tcaggggaat tattcaacac ccgaacatgc tgaaataatt ctgttgcttc atcgtattct
    40981 ttacgcaaat aactcaacca ctgtttaatc cgcgctacgt gatataaccc ggtatcgccc
    41041 tgcttttcca gacgggtata tttttgcagc aaagcaacca cctccggcca cggcattcgc
    41101 ggttcgttat attttactac ccggctcagg ttgggaatat tgagcgcccc gcgaccaatc
    41161 atcactgcgt cgcagccgct gatcgcaatg cattgttgcg cgctctgcca gtcccagatt
    41221 tcgccgttgg caatcaccgg aatattcagc cgctggcgaa tctcgccaat cgcctgccag
    41281 tcaatatgct ccgcgcggta accctgctct tttgtccgtc catgcaccac cagctccgta
    41341 gcaccagcct gttgaaccgc atcggcgatt tcaaatttct tctcaccgct gtcccagccc
    41401 agacgcactt tcacgctgac gggcaaatgc gccggtacag cttcacgcat cgcttttgca
    41461 ccctggtaga tgagttcagg atctttgagt aacgtcgccc caccaccgct accgttaacc
    41521 gttttcgacg ggcagccgca attgagatcc acgccccagg aacctaattc caccgcacgg
    41581 gcggcgttct ctgccagcca ttgtgggaac tgacccaaca actgcacgcg caccagcgta
    41641 ccggatggtg tccggctggc gttttgtagc tcagggcaaa tgcgatgaaa gacttttatc
    41701 ggcagcagtt gatccaccac gcggacaaac tcggtgatgc acagatcgta gtcgttaact
    41761 tcggtcagca attcacgcac cagagagtca agcactccct ccatcggtgc cagtaacaca
    41821 cgcatatcat cacccgcaaa aaaatgaggc gctatgttag cgcctcctgt gtttgcagac
    41881 aaactgactt actgattaat tcgggcaaga tttttaccct caccctaacc ctctcccgaa
    41941 ggggcgaggg gaccgcctga ttgggtgaag aagtgctcag cgtggttgca aacagttatc
    42001 ttctttccag ccgtacaacc actccatgcc gcgataaaat tctgcctgcg atttcccttt
    42061 ccacagcaga ttacgtacct ggcgttctac ccctgcggcg tggtaaaccc gccccatttc
    42121 acgggtcgac cagacgatgc gcgcggtgcg cggaattcgt accgactcat acagcgcaaa
    42181 ggcctgcgcc gcatcaccct cacactgcgc cagcgcctta cccagcgtta ctgcatcttc
    42241 cagcgccata caggctccct gcgccatata ttgcgccacc ggatgggccg aatcgcccac
    42301 aagcgttatg cgcttcgttc cccacttcgc caccggttca cggtcagcgg ttgaccagcg
    42361 tcgccatgaa gtcggtttat ccaacatctg gcgcggacgg gggtgaatcc cttcaaagta
    42421 agagagcact tcctctttac taccatcctt cacgccccac tcttcctgct ggcggctgtg
    42481 gaatgttacc accaggttat actgcttgcc gccgcgcaaa gggtaatgca ccaagtgaca
    42541 atgcggcccc gcccacagta ccggtgcgtt aatgcgcaaa tcttccggca tatcggcacg
    42601 atcgataacc gcacggtaga caacatgtcc ggtgacacgc ggcgcatcgc ccaataagct
    42661 ctggcgcacc actgacttca cgccgtcaca gccaatcaga atatcgccgg tccagctatt
    42721 gccgcgatcg tcaaacacgg tgacatcgtc ttccgtctcg cgaatatcca cgacgttggt
    42781 ggaggtgtga tattccacgt ccggatgcgt cagtaccgct tcccacaccg aggcgtgaat
    42841 atcaacccgg tggatcaccg cgtaagggcc gccgaaatgg tcacgaaagg cctgcccggt
    42901 ttcaatgcac accacttctt cggcgttcac cgcatccatc atggtaatgt gatcggtaaa
    42961 caccgcgcgc tggcgggcaa cttcgccgac gccgaggctg tccagcgccg aaaacgcatt
    43021 tggccccaac tggatgcccg cgccaatctc accgatctca tgcgcctttt ccagcagcac
    43081 cactttgatc cccaggcggg ccagtgacag cgcagttgcc gcaccgccga tcccgcctcc
    43141 cacaattact gcgcgtgtca ctttcgtcat tatagttctc ctggtcaggc tgaaatttta
    43201 tcttgttgat tttctggcgc tgcggcaatg aacgctggca gctgtgtgca ggcgtcgtac
    43261 accgctttgc agcgtggata cccgctcaaa tcgcagttca tgcgtagcgc gttcgcccac
    43321 tgcgggacaa ggcagcaatc tgccagcgtc ggcgtctcgc ccacacaaaa ttgccccgac
    43381 tggttctggc gcaacagttg ttccaccgcg cttaaccctt gctggatcca gtgcgcatac
    43441 caacgttttt tttcttcttc actgacgttc agttcttcgg tcaggtagcg cagcacgcgc
    43501 aagttattca atggatgaat atcacaagcg atggcataga caatttccag caccttgttg
    43561 cgggccggtt cttcctgcgg caccagcgga acttgcgggt agtgcctctc cagccagtca
    43621 ataatcgcca gcgattgtcc gagtgattcg ccctcgtcag tcagcagcgt cggcaccagc
    43681 ccgaccggat tcatccgccg ataggccagt tcattttgct gaccaatacg gatattcacc
    43741 ccttcggtct ggtaattaat gcctttcagc gccagcgcaa tgcgtacccg gtacgacgct
    43801 gaactattaa aaaaactgta cagcttcatc atgcacctca gacaatcttc accgtgatag
    43861 gcgttaagcc ctcgacgttg cctgtgatca catcaccttt cactaccggg ccaacccctt
    43921 ccggcgtacc ggtgaagatc agatcgcccg gttgcagctc aaagaagcct gacagatagc
    43981 tgatggtttc attcaccgac cagatgagat ggcgaatatc gctgcgctga tgatcttcgc
    44041 cgttaacctg taaccagatt ggcgcattat caataccgcc gatatcgcgg gctttgtgca
    44101 gcggcgcgat gggcgctgag agatcaaacg ctttaccgat ttcccacggg cggcccatct
    44161 ggcgcatttc catttgacgg tcacgccgag tcatatcaag acccgtggca taaccccaga
    44221 catattcatg ggcgtgttcc agcgggatat cgctgccttt tttaccaatc gccaccacca
    44281 gttcaatttc gtaatggtag ttatcggtct gtgccggata cggcagcgcc agcgtttcac
    44341 cagcggcaac cggaacaacg gcatccgccg gtttgcagaa gaagaatggc ggttcacgat
    44401 ccggatcaaa gcccatttcg cgggcgtgag cggcatagtt acgtccgacg cagtaaacgc
    44461 ggcgcacggg gaattgttca tcactgccaa caaccggtac ggtaacgggc gcttgcggtt
    44521 cgaatacgta ttgagtcatt ttttctctcc taaattaata acgcgcttcg cggaacagcc
    44581 ccagggcttc ttgtaccggt ctgtccgaaa aactaaataa aacggaatct tgcgtggtct
    44641 ggaacgacac gccatgccag gtcggcacca cgaagatatc ttttgcacta aagctgaagg
    44701 tttcattgcc gatgatgacc tgcccgctac cttccaccac atggtagatg gtgctgtcag
    44761 tggtacgcgc aacgcgtgag gcaaaccctt tcggcagcag ttgcaggaaa gcgcccatcg
    44821 acggcatcgg atagccgccg gtgaccgggt tgacgtagcg cattttgtag ccatcccact
    44881 cgtcggcatc gcccagtcgg gttaaatcgt gcagcacttc gcggctgcgg tcataacgat
    44941 agttaaagat cggcgaggag ttccccgtct gatgccgcag cggcaacata ttggcagcgt
    45001 aacgcggcag ataatctccc tctttacgcg ttaccggttg ttgctcttcc ggataatctt
    45061 cggcaaaacc gcagcccaga atattcacta atggcagatc cagcccatcg agccagataa
    45121 ccggttcgtc gccagggtta ccgtgatcgt gccagcgcca ctgcggggtc aggataaaat
    45181 cgccttcatt cattggcgtg cgttcgccat caacggcggt aaatgcccct ttgccttcaa
    45241 caataaaacg cagcgccgac tgattatgac gatgactcgg tgccacttcg cccggcatga
    45301 tcagttgcaa accggcatat aaagtcgcgg taatggaaga ctgcccgcgc agcgccgggt
    45361 tttccagcac cagcacgcgg cgcacggcct ctttcgcacc aatcaggccg ccgctctcca
    45421 gtagcagcgg gcggatctcc tgataattcc agtacgctgg cgcacagtta gcgttgggcg
    45481 ttttcggcac cagatggtgc agtgactccc acagcggggt caggttttgc ccggaaatat
    45541 gctgataaaa ttgctcgcgg ctattttgat tgttatcagt cattgggatg ctccttattc
    45601 gttcacaacg cctgcgcgag gcagtgagtc agggacagag gtcgactggc gaaccacggt
    45661 aatcagcagg gttaacatca ccgcactgat cgccgcaggc acagcgataa tgaaaaacag
    45721 ggtgtcgaaa gagaaattca tcgccatcat cacgccgcca gacagcgaac caacaatcgc
    45781 gccgcagcgg ccgatagcat ttgaccagct cacgcccgtg gcgcgacttt gtgttggata
    45841 aagtgttgcg gtcagcgcat tcagccccac ctgcgatccg ctaatgccaa tgccagtgcc
    45901 gaaaatcgcc agcgccatca accataatcc atcctggctt aagccaatca tcacgatgca
    45961 tatagcgcct atggcatagc tcagcgccag cacgcggaaa ggattgaact tatccatcag
    46021 cacacccagc gccagcgcgc ctaacgtacc accaatctgg aacgcggcgg tgacccagga
    46081 cgcgtgttgc aggtcgatgc cgcgatggtt cagtagcgtc ggcatccagc ttgagagcag
    46141 gtaaataatc agcaggctca taaagaacac tacccacagc atcaacgtga tgggtaactg
    46201 acgtcccata aacagttgac gaatgctgcc ttttgtaacg gaggctgatt caatgagata
    46261 aaagtgggta tcgacataac gctcgcgggt gatggcgctg acggttttcg caatcaccgc
    46321 ctgcggtagc tggcggcgca cctgccagcg tggcgattcc ggcagaacta ccagcagtgc
    46381 gacaaacagc atcagcggta acacgccccc caacaccaga atgccgtgcc agccaatcac
    46441 cggcaccagc tgcgcactga caatcccgcc aaacgccgaa cccagcgtaa agccgcaaaa
    46501 catcagcgtc accagcgcgc cgcggcggcg ggcaggcaga tactccgagg tcatggtgat
    46561 ggtgttcggc atcgcgccgc ccagacccag gccggtgaga aagcgcagga agaccagcgt
    46621 ttgcagatcc ggtgagaaag ccgaggcgag gcttaacgcg ccaaacaaaa agacgcacag
    46681 ctcaatcacc cgcttgcgac caaagcgatc ggacaacggg ccacaaagca gtgcgcctgc
    46741 ggttaatcct agcagacccg cgccaaaaag cggggcgaga tcgcctgcgg tgagttgcca
    46801 gtgagtgcga atatcagggg cgataaatcc gatagccgcg gtgtcaaaac catcgagcat
    46861 taccaccaga aaacagcaga tgataacgcg ccactgcatc ttgccgaccg gagcagcatc
    46921 gataagtgct tgtaaatcac gtcgttgagt catagtaaat gcctcagtgc aggtaaggta
    46981 gatgtttctt ttgttttgta tatgttgcga tattgtgatt aaagagatga gatgtacaat
    47041 ggcatttcgg gcagagagat aacctggagg ttatggcaaa tggcgaactg ggcgcaaaaa
    47101 ttaaaactac atcaccttga aatgctggtg gcgctgggtg aacagggcaa tctaacccat
    47161 gtcgcacgaa tgatgaatat cacccaacct gcgctttcga aatggttgtc acaactcgaa
    47221 gatgagattg gaatcacact ttttgagcgg cacagcaaag gattacgtcc gactgaaggc
    47281 ggcaaattgt tattgcaaca tgcccaacgg ttaatcaatg acatggagcg atcgcaacat
    47341 gaactggccc gttttaaaca aggtggactg gtcggcagtc tgaaaattgg ctgttctccc
    47401 gtcgccactg actgcgtatc ccaggcgatt ttgcatttgc ttacgaagat gccaactcta
    47461 cacctgaata tcgaagagaa ggtgatgacc ccattgctgc aagatctatt ggcaggttct
    47521 gtcgatgtgg tcgttgggcg aattggcggt cgcgcattgc agctgccact gaattatcag
    47581 gtgctgtaca ccgagccagt atgctttgtc gcccgccctg agcacccact ggcgaaatac
    47641 gccaccctca gctggaatga tcttgccaac tggcgctgga tcgtctggcc gacgggtacc
    47701 cccattcgca ttagtattga taatgcgctg gtagaccacg gcgtaatgct gccagaaaac
    47761 acgattgagt cggcgtcgat gaacgtcagt accaacctgt tacaaagcag cgatatgatc
    47821 tctattttgt cattacgcct ggcgcaacgt tatgccagcc agggacaact ggtgatactc
    47881 aacctgccaa agatagaaca gaaaggtagc gtcggagtgt tctggcgcaa taacgaaacg
    47941 ccttcttatg ctttaagtca gtttctgcac tttttacagc agaattagtg tgctgaacgg
    48001 ggcgctcgcc cgaggttacc tccctaaaat tcaactatgc tgagaattct taatgtgatc
    48061 ggtagcacgt tttacgaatt aattgtatga tgaatccatc tcatctgggg tgttgattat
    48121 gagtaagaca ctgaacatta tctggcaata tttacgcgct ttcgtcctga tttatgcctg
    48181 cctgtatgca ggcattttca ttgcttccct gctaccggta accattccgg gcagcatcat
    48241 cgggatgctg atcctgtttg tcctgctggc cttgcaaatt cttccggcga aatgggtcaa
    48301 tccggggtgc tacgtactga ttcgctatat ggcgctattg tttgtgccga ttggcgtagg
    48361 cgtcatgcaa tattttgatt tgctccgcgc acagtttggc ccggtagtgg tttcctgtgc
    48421 aatcagtacg ctggtggttt ttctggtggt gagctggagt tcgcaactgg tacacggtga
    48481 acgtaaagtc gtaggtcaga aaggatcaga agaatgatgg cgaatatctg gtggtcatta
    48541 ccgctgacgt tgattgtctt ttttgccgcc cgcaaactgg cggcacggta taagtttccc
    48601 ttgcttaacc cgttgctggt agcaatggtg gtgatcattc cttttttaat gctgaccggc
    48661 atctcttacg acagctactt taaaggtagc gaagtgctaa acgatctgct gcaaccggcg
    48721 gttgtcgcgc tggcctatcc tttatatgag cagctacacc agatccgcgc gcgctggaaa
    48781 tcaatcatca ccatctgttt tatcggcagc gtggttgcga tggtgacggg gacttccgtg
    48841 gcattgttga tgggcgcttc accggaaatc gccgcgtcaa tcctgccgaa atcagtcacc
    48901 acgcctattg caatggcggt tggcggcagt attggcggta ttccggcaat cagcgcagtt
    48961 tgcgtgattt tcgtcggcat cctcggcgcg gtatttggcc atacattgct taatgcgatg
    49021 cgtattcgta ccaaagctgc gcgtggtctg gcaatgggga ctgcctcgca cgccctcggt
    49081 acggcgcgct gcgccgagct ggattatcag gaaggtgcat ttagttcgct ggcgctggtg
    49141 ttatgcggga taattacttc gctgatcgca ccgttccttt tcccgattat tctggcagta
    49201 atgggctaaa atttgcgatg cgtcgcgcat ttttgatgta agtttcacgc gttgcataat
    49261 taatgagatt cagatcacat ataaagccat aacgggttcg taaactgtta tcccattaca
    49321 tgattatgag gcaacgccat gcatccacgt tttcaaaccg cttttgccca acttgcggat
    49381 aacttgcaat ctgcactgga acctattctg gcagacaagt acttccccgc tttgttgacc
    49441 ggggagcaag tctcatcgct gaagagcgca acggggctgg acgaagacgc gctggcattc
    49501 gcactacttc cgctggcggc ggcctgtgcg cgtacgccat tgtcgaattt taatgttggc
    49561 gcaattgcgc gcggtgtgag cggaacctgg tattttggtg ccaatatgga atttattggt
    49621 gcgacaatgc agcaaaccgt tcatgccgaa caaagcgcga tcagccacgc ctggttgagt
    49681 ggtgaaaaag cgcttgcagc catcaccgtt aactacacgc cttgtggtca ctgccgtcag
    49741 tttatgaatg aactgaacag cggtctggat ctgcgtattc atctgccggg ccgcgaggca
    49801 cacgcgctgc gtgactatct gccggatgcc tttgggccga aagatctgga gattaaaacg
    49861 ctgctgatgg acgagcagga tcacggctat gcgctgactg gtgatgcgct ttctcaggcg
    49921 gcgattgcgg cggcaaaccg ttcgcacatg ccttacagta agtcgccaag cggtgttgcg
    49981 ctggaatgta aagacggtcg aattttcagc ggcagctacg ctgaaaacgc cgcattcaac
    50041 ccgactctgc cgccgttgca gggagcgtta attctgttga atctcaaggg ttatgattac
    50101 ccggatatcc agcgcgcggt tctggcagaa aaagccgatg caccgttgat tcagtgggat
    50161 gccacctccg caacgctgaa agcgctcggc tgtcacagta tcgaccgcgt gcttctcgct
    50221 taagcctggt gccggatgcg gcgtgaacgc cttgtccggc ttgccagccc tctcctggtg
    50281 tcgaaaatcc cggcaaacag tttgccgttt cttgcgcaaa accagcgggt aaagtagcct
    50341 gatggaaatt ttccttagat cgagtctcct gcatgttaaa gcgcgtgttc ctcagcctgt
    50401 tagtccttat cggcttgctg ctgttgactg tgctcggcct cgatcgctgg atgagctgga
    50461 aaaccgcgcc ttatatctac gacgaattgc aggatctccc ctaccgccag gtcggtgtgg
    50521 tgctcggaac agcaaaatat tatcgtactg gcgtaattaa tcagtattat cgctaccgca
    50581 ttcaaggagc gattaatgcc tataacagcg gtaaggtaaa ttatctatta ctgagcggcg
    50641 ataacgcatt gcaaagttat aatgagccga tgaccatgcg caaagattta atcgctgctg
    50701 gtgtcgaccc atcagatatt gttctcgatt acgcaggctt tcgtacgctg gattccatcg
    50761 tgcgtacacg caaagtgttc gatactaatg atttcattat tatcacccaa cgtttccact
    50821 gtgagcgggc attatttatt gcgctgcata tggggattca ggctcagtgt tatgccgtac
    50881 cgtcaccgaa agatatgctg tcagtacgta ttcgcgaatt tgccgcccgt ttcggtgcac
    50941 tggctgacct ttatattttt aaacgtgaac cgcgtttttt agggccgctg gtccctattc
    51001 cggctatgca ccaggtgccg gaagatgcgc aggggtatcc cgccgtcaca cccgaacagt
    51061 tacttgaatt acaaaagaaa caaggaaagt agttatggat gtacagcagt tttttgtcgt
    51121 tgccgttttt ttccttatcc cgatattttg tttccgcgaa gcatggaaag gctggcgcgc
    51181 aggcgcgatt gataaacggg ttaaaaatgc accggaaccg gtgtatgtct ggcgagcaaa
    51241 aaatcccgga ctctttttcg cttatatggt ggcatatatc ggcttcggaa ttttatctat
    51301 cggcatgatt gtttatctta ttttctatcg ttaatatttt ctcttttctg ttaattattg
    51361 agaataatta ttacttcact caataagctg cggatataac attctgaacc gcagctattt
    51421 gtgaatcctt tcacagttta aatttctcca cgcgcatagc cttaatatca gcacgcaata
    51481 tttatttagc gttcgcctta attactcata gcattaagga agatcacatg ccgcaacaaa
    51541 attatctgga tgaactcact ccggctttta cgccgttact ggcgattaaa gaagcctctc
    51601 gctgtttatt atgtcacgac gctccctgta gtcaggcttg cccggcacag accgatccgg
    51661 ggaaatttat tcgctcaatc tactttcgta attttaaagg cgctgccgag acaattcgcg
    51721 aaaataatgc cctcggtgcc gtttgtgcca gagtgtgccc gacggagaaa ttatgccaaa
    51781 gcggttgtac ccgtgccggt gttgatgctc ccattgatat cggccgctta cagcgttttg
    51841 ttactgattt tgaacaacaa accgggatgg aaatttatca gcccggtact aaaacgctcg
    51901 gcaaagtcgc gattattggc gcaggtcctg ccggattgca ggccagtgtg acactgacaa
    51961 accagggtta tgacgtcacg atttatgaga aagaagcgca ccccggtggc tggttgcgta
    52021 acggtattcc gcaattccgg ttaccgcaat cagtgctgga tgcagagatc gcccgtattg
    52081 aaaaaatggg cgtgaccatt aagtgcaata acgaaatcgg taaaacactc accctggagc
    52141 agctgaaagc ggaaaaccgc gcggtactgg tcaccgtggg gttatcaagc ggttccgggc
    52201 taccgctgtt tgagcatagc gacgttgaga ttgccgtcga cttcctgcta cgtgcacgac
    52261 aggcgcaagg cgatatcagc attccacaaa gcgcattaat tatcggcggc ggtgatgtcg
    52321 cgatggacgt agccagcacg ctgaaagttc tcggctgtca ggcggtaact tgcgtagcgc
    52381 gtgaagagtt agatgagttt ccggcaagcg aaaaagagtt tgccagcgcc cgggagctgg
    52441 gcgtttcgat catcgatgga ttcacgccag tagccgtgga aggcaataaa gtcacgttta
    52501 agcatgtacg gttatcgggc gaactgacga tggtggcaga taaaattatt ctcgccgtcg
    52561 gtcagcatgc cagactggat gcctttgcgg agttagagcc gcagcgtaac accatcaaaa
    52621 cacaaaatta tcagacccgc gatccgcaag tctttgctgc tggcgatatt gttgagggtg
    52681 acaaaaccgt ggtctatgcc gtgaaaaccg ggaaagaagc cgccgaggcg attcatcact
    52741 atttagaggg agcttgctca tgttaacgaa agatctttcg attacttttt gcggcgtgaa
    52801 gtttcccaac ccgttctgcc tctcttcttc gccggtaggc aactgctatg agatgtgtgc
    52861 caaagcctac gacacaggtt ggggcggtgt ggtgtttaaa acgatcggct tttttatcgc
    52921 caacgaagtc tcgccgcgtt ttgatcatct ggtgaaagaa gataccggtt ttatcggctt
    52981 caaaaatatg gagcagattg ctgaacatcc gttggaagag aatctggccg ccctgcgtcg
    53041 gctgaaggaa gattacccgg acaaagtatt gatcgcttcg atcatggggg aaaatgagca
    53101 gcaatgggag gagctggcgc gcctggtgca agaagctggc gcggatatga tcgagtgtaa
    53161 cttctcctgt ccgcaaatga cttctcatgc gatgggtagc gatgtcgggc aaagcccgga
    53221 gctggtagaa aaatattgtc gggcagtgaa acggggttcc acgctgccaa tgctggcgaa
    53281 gatgacgccg aatatcggtg atatgtgcga agtggcgctg gcggcgaagc gcggcggcgc
    53341 agatggcatt gcggcgatta acaccgttaa atccatcacc aatatcgatc ttaatcagaa
    53401 aatcggtatg ccgatcgtta acggaaaatc gagtatttcc ggatattccg gtaaagcggt
    53461 aaaaccgatc gccctgcgct tcattcagca aatgcgcacc catccagaac tgcgcgattt
    53521 cccaatcagc ggtatcggcg gcattgaaac ctgggaggat gcggcggagt ttttattgct
    53581 cggcgcagca acgttacagg tcaccaccgg cattatgcag tacggctatc gcatcgtaga
    53641 agatatggcg agcgggttat cgcattatct cgccgatcag ggatttgatt cgttgcagga
    53701 gatggtaggt ctggcgaata acaatattgt ctcggcggaa gatttagacc gcagttatat
    53761 tgtctatccc cgtatcaatc ttgataaatg tgttggctgt ggacgctgtt atatttcctg
    53821 ttacgacggc ggtcaccagg cgatggaatg gagcgagaaa acccgcacac cgcattgtaa
    53881 taccgagaaa tgtgtgggtt gtctgctttg tggtcacgtc tgcccggtgg gttgtattga
    53941 gctcggggaa gtgaagttta agaaaggcga gaaagaacac ccggtaacgt tgtaaaacca
    54001 gagacgcatc cggcatttgg ttcctgtcgc cggatgcggc ttgaacgcct tatccggcct
    54061 acaaaaccat acaaatcagt atgttgagct cattgtaggc cggataaaac gcgccagcgt
    54121 tgcatccggc atttggctcc tgtcgccgga tgcggcgtga acgccttatc cggcctacaa
    54181 aaccacacag atcatgatgt tatgtgcgtt tatgaccgaa tgcgaaccac aatcatatca
    54241 cttcttacgc gcgtacttca gtgaatccag tgccacggcg aagataataa tcgccccttt
    54301 gatgatgtac tgccagtacg ggtttacacc gatgtacgtc aggccatagt tgatgacggt
    54361 aaaaataatt accccggtca ccacgccaat caccgtcccc acaccgccgc tgaacgatac
    54421 gccgcctacc acgcacgccg cgatagcatc cagctcatac ataaagccga ggttgttggt
    54481 ggcagagccg atacgtccgg cttctaacat cccgccaaag gcatagaaca cgccagacaa
    54541 cgcgtagatc atcagcaggt tcaggccgac gttgacacca gatacttttg ccgcttccgg
    54601 gttaccgcca atggcaaaaa tgttcttacc gaagcgggtt ttgttccaca acacccagac
    54661 gaacgccacc gcaatcaacg cgtagaaggt gatgtaagag agacggaaac tccccagcgc
    54721 gacaaagccc tgagcaaagg tagagaagcc actgtcaaaa ccagaaattg gcgacgcccc
    54781 gacgaaatcg taatagagcg agttgatgcc atagacgatg atcatcgtgc caagcgtggt
    54841 aatgaacggc gtaacgttga gataagcgat aatcagaccg ttgatcaaac cgatcaccgc
    54901 accaatggca cagacaatca gaataaccag cgcaatcggc atcgtcgcca tttccgggaa
    54961 cactttgttg gcgttatcca tggactgcaa taatgtcgct gccaccactg ccgccagccc
    55021 tacttgacgc cccgcagaaa ggtcggtccc ctgggtgaca attaaccctg ccacaccgag
    55081 cgcgataata atacgcaccg atgactgggt gagaatatta cttaagttca acagacttaa
    55141 aaatgttggg tcctggaaaa taataatcgc cagcaaaact aaaagaacga cgtaaatacc
    55201 gccctctttc aggtaagtaa gaaaactttt cttatttaac gcactcatgg gaagccccta
    55261 atcttaaagg tgcaaagacg caagacgcag aatttcgttt tgcgttgttg tttttgtatc
    55321 gacaattccg gaaacgagac cattgctcat gaccagaata cggtctgtta tccctaacaa
    55381 ctcaggcatt tcagaggaga taataataat ccccttgcct ttcttcgcca gttcggcaat
    55441 taactgatag atctcaaact tcgccccgac atcaataccg cgcgtcggtt catcgagcat
    55501 taatatttct ggttgcgtta gtagccagcg accaataatc accttttgct gattaccacc
    55561 tgagagcgaa ccaatttgcg tccgatgccc cggtgttttt acccgcatcg aatcaatcac
    55621 ccactgagta tcgcttttca tccgcgagtt atccagtaaa ccaactttat ttttgtagtt
    55681 gcgaatattg gaaattaacg agttaaaacc aatatccaga taggcataaa ttcctgttga
    55741 gcggcgctcc tcagttacca gtgcaaatcc gtggtttatg gcttcgttgg cattatggtt
    55801 attgatcttt ttgccgtgca acgtaatggt gccagccgat ttctcgcgaa taccaaataa
    55861 cgtctcaaca atatcggtac gtttcgcccc caccagaccg gcaataccga ggatctcccc
    55921 tttatgcaga tcaaacgaga catcacggat cgacggctgg cgcagtgacg tcaggttacg
    55981 cacctcgagg atgacttcgc ccggcttgtt ttctttgtca gggaaacgct ggttaagaga
    56041 acgcccaacc atcatggcga tgatcttatc catcgtcagt cctgccagcg gttcggtggc
    56101 gatccactga ccgtcgcgca atacggtaac ttcatcacat aactggaaga tttcttccat
    56161 tttatgcgag atataaacaa taccgcagcc gcgctctttt aatttacgaa taatagtgaa
    56221 cagatgattg acctcttttt cggttaacga agaagtcggt tcatccataa tcacaatttt
    56281 cgcgttatag gaaaacgctt tggcgatttc gatcatctgc atttgcgaaa cagataatgt
    56341 gccgacgcgc gcacgcggat cgatatcaat atccagttca tcaaaaatcg ctttggtttc
    56401 gcggtacatt ttgtcctgat cgacaaacat gcctttggtg ggatatcgcc ccagccacat
    56461 gttatccatc accgaacgtt gtaataccag gtttaactcc tggtgtacca tcgaaatacc
    56521 attttccagg gcttctttgg ccgaatggaa atcgatctct ttaccctgga ataaaatggt
    56581 gccggagtct ttttgataaa taccaaacag gcattttaat aatgtcgatt ttcctgcacc
    56641 gttttccccc attaatgcat ggatagaatg tggccggact tttaaattaa cgttatcaag
    56701 tgccttaaca ccaggaaagg acttgttgat accgctcatt tccaacaagt attccccgga
    56761 agacggagtc gttgagctga ccatataatt ttaccttgtt ggccatacaa taagggcgca
    56821 gtaatagact gcgcccaatc agtcttattt cttgctgaat tcagccaggt tgtctttatc
    56881 tacgccaaca taaggtacgc ggaccacttt gttgtcgatt ttccagttgg tgccatcagc
    56941 cgcaccttta ccatcggcca ggtttttcgc cagatcaaag gtcgctttcg cctggttgtt
    57001 agcatcgttc agtacggtgc ccgccagtgc accggatttc accagcgcca gcgcttctgg
    57061 cagagcatcg acgccaaaca ccggaatgct ggacttgttg tgtgctttca gtgcttctac
    57121 cgcgcccatt gccatcgcat cgttgttggc gataaccact tcgattttgt tggcgttcgg
    57181 gccagacagc caggcgtcca tcttatcttt cgcctgagcg gtatcccaca tagcggtatc
    57241 taactgtaac tgttcagttt taatgccttt gtcgttcagc tctttaatca cgtaagtggt
    57301 acgtgcttct gcatccggat ggcccggttc acctttcagc agtacgaatt gaatctgacc
    57361 gtctttattc agatcccaac cctgattcgc cgcccagtgt ttagcaatca aatcgccctg
    57421 aataatgccg gactctttgg agtcagtgcc aacgtagtag gctttgtcgt agctatccag
    57481 cgccttacga gacggctctt tgttaaagaa aaccaccggc acgttttgcc cacgcgcttt
    57541 ctcaatcacc gtacccgcag ctgccgggtc aaccaggttg attgccagtg ccttcacccc
    57601 tttcgccagc aatacgtcga tctgatcgtt ctgcttggac tggtcattct gagaatcatt
    57661 catcagcagc tgaacatctg gcgcggcttt cgcatcctgc tcaatagcct tgcgcaccac
    57721 agacataaag ttatcgtcgt acttatagat tgttacacca atgcgagtat cagcagcgtg
    57781 tgcagcggca ccgaataaca tgctggccat cacagcagac agggttaaca ccttcttatt
    57841 catggtatct ccggtttttc ttatgcaggg tagtgcttga gataaatgct cggcggggca
    57901 gtagagttaa tgaagtgtta ctgaacgccg aagctcactt tttaaaattc gttcttccat
    57961 gcccggtaac gctccagaaa acggctttaa ttgttgttat gacgctatta cctcggtgaa
    58021 agtgattatt cactgttaca tacgggttac aacgttaaaa cggtgcaatc atagctatca
    58081 cattgttaag atactgtgaa atcactcaca gattgaaagc ggttacatcg ccagatttgt
    58141 tgagttagtg atcgccgccg cattctggcg cgttgcgaca gaatggcgac gcactaacgt
    58201 cggcataaaa cagtggctgg cacgaggatc aatattgcct gctgcaccct gcaaggccag
    58261 ttcggtggct aatttagcca ttgaagcaat gggataacgc acggtcgtta attgcgggtc
    58321 ggtgtaacgg gcgatgggaa tatcatcgaa accgatgatt gagagatgta acggaatcgc
    58381 aatgccatta tcttttaatg ctgtcagtgc gccagcggcc atattgtcgt tataagcaaa
    58441 tacagcggta agttgtagat tgcgccccag cagttcaacc atcgccgcct ctccacccgg
    58501 catgtccggc gtgccagtgc caatccagct ttccggcgga ataatatcct gctctttcaa
    58561 cgcactcatc cagcctgctt tacgcatggc gtcatcttca atgccgtgac tggaagaaag
    58621 ataaccaata cgttgatgtc cgttattcag caacatgcgc gttgccattc gggcaccgct
    58681 gagattatcc aggcaaacgc aacgatgggc gtaccccggc acaacgcggt tgattaacac
    58741 cataccggga atgttatcca taaattgcgc cagttcatcg tcactcaatg cttttgagtg
    58801 aacaatcaat gcattacaac gctggcgaat taacacctca atggcgtgac gctctttttc
    58861 cgcttcatga tagctattgc cgattagcac gtatttctga tgctgctggg cgaccagatc
    58921 caccgctttt accagcgcgc cgaaaaacgc atcagaaacg tccatcacca ccacgccaat
    58981 ggtgtcgcta acctgagttg ccagcgcctg ggcattggcg tttggccgat aatccagctc
    59041 actcacggct ttcattactg cttcacgcgt gtcggcactg accagcgtgc tgttattgag
    59101 cacccgggaa accgttgcca cagagacgcc agcctgacgc gctacatcac gaatggtgat
    59161 catattcact acctgttatg agattgcagt aaatgactgc ttgctggcgg ctattttgtc
    59221 agcactgaat acaggacttc gtgaatcgag tcacagcaat ggaaacggtt acagccgttt
    59281 tgttaacgaa tgtgatccag atcgttatct tgatgtttta gatattgctg gtccggcagc
    59341 acgcaaagtt aactgacgcc agagccattc caccggcccc tggcggaagt aacgcagcca
    59401 gataacagag aagagaatat tcgccagcca taccggaata acaaacgcca gcaattccag
    59461 acggtcaaac tgcataaaca aaccgaggtg ataaaaaagc gtggtacaaa tcagcgtttg
    59521 caatagatag ttggtcagcg ccatccgtcc gacgcaggcg atcgcaagca ccagcttaaa
    59581 gcggctcaat tgcggccaga aaccataaaa cagcgatgca tagccaatcg cctgaaacgg
    59641 tgcactcagt tcccgcggta tctgaagtaa gaaggcgcac cagcgatatg cccagtccag
    59701 ccgccattgc agggcgatgg caggaaggtt aatgatcacc ccaatcgcca ccagcacaaa
    59761 accagtacga cgatagtgac gtaagctgaa ctgccctttc agccagccac tgcgcatcaa
    59821 tgcggcacca atgagcatca tcccagccag ttgccagcca tactgtgcgc ccagtgccag
    59881 taaactgttg ccaacaccat cggcacgatt actgatcgct tcaacgccgc cgtgaagctt
    59941 ccagtatttt tcatacaaaa tagccgatgc atccggcgtc caggcgcggc tggtctggct
    60001 gtcggaaatc agccccaata acagcaaaac gccaagcccc accagataaa gcatgacgcc
    60061 ggtattaaaa aggcttttta ccgatggcgc atcgcgcacc agccgccagc agattaagcc
    60121 caccagcccg taagccagca gaatatcgcc gtcccagaac aataaaccgt gaataaagcc
    60181 caacaagact aacagcgtta accgcgactg gatccagcgt ctgccacggg gcagcaacat
    60241 ttgcaggcca gcaccaaaca gcagcgcaaa aagcgtgagg aatttcacct ggccgatgag
    60301 atcgagaaat gcccaggtcc atgcatcctg cggcgtaata gcgccgtacc aggcgggatt
    60361 aagataagcc gcctttggta gcccaaaggc gctgatgttt aatagcagga tccccagaat
    60421 ggcgacgccg cgaacaaaat cgagcgtgac gttgcgctcc atggttcctg ccttttaatc
    60481 agttgtgatg acgcacagcg cgcagaaact cgtggcgcgt attctgactg gatttgaaca
    60541 atccaccaag agaggtcgtt gtcgtggcac tggttgcatc gcggatgcca cgcgccttca
    60601 cgcagtaatg caccgcgtcg atagagacag ccacgttatt ggtgcccaac aacgtttgta
    60661 gcgcaatgag aatttgctgc gtcagacgtt cctgcacctg cggacgctgg gcaaagaact
    60721 gcacgatacg gttaattttt gacagaccga tcaccgaatc tttcgggata taagccaccg
    60781 tcgctttgcc atcgatggta acaaaatggt gttcacaggt actggtcaga gtgatatcgc
    60841 gcacggtgac catttcatcg accttcattt tgttttcaat gagggtgatt ttcgggaaat
    60901 tggcgtaatc cagaccggag aaaatttcat cgacatacat tttagcgatg cgatgcggcg
    60961 tttccatcaa actgtcatca gccaggtcga gattcagcag ctgcatgatt tcggtcatat
    61021 gaccagcaat aaggcttttg cgcgtttcgt tatccatttc atgcacgggc gggcgcagcg
    61081 gtgtttccag tcctcgcgca actaacgctt catgaaccag ggccgcttct ttactgagtg
    61141 atggcattta tgatttctcc tgcaggtgtg acgcctccgc cctgcgtggg ggcaaagtta
    61201 ttaagctgat ttacagcctg attattgtgc gtgaggcggc gcacataatc cagtattcac
    61261 agcgataatt attgtaattg ccgctgcctt tcatcagcag atgttaaaac atcgttatgc
    61321 aaatacggaa gtgaaagtta ctcacagcac attgaataaa cggtatgatg aagaaattgc
    61381 aaacaacaca acaaggagcc acgcatggaa atgctcgaag agcaccgctg ttttgaaggc
    61441 tggcagcaac gctggcgaca cgactccagt accttaaact gcccgatgac gttcagtatc
    61501 tttctccctc cacctcgtga tcacaccccg ccaccagtgc tgtactggct ttccggatta
    61561 acctgcaatg acgagaactt caccaccaag gcaggtgccc agcgcgtggc ggcagaactg
    61621 gggattgtac tggtgatgcc agacaccagc ccgcgcggcg aaaaggttgc caacgacgat
    61681 ggctacgatt taggccaggg cgcaggcttt tatcttaatg ccacgcaacc gccgtgggcg
    61741 acgcattacc ggatgtatga ttatctgcgc gatgaattac cggcgctggt tcagtcgcaa
    61801 tttaatgtca gcgaccgctg cgctattagc ggtcattcaa tgggtggtca cggtgcgctg
    61861 attatggcgc tgaaaaacca gggcaaatac accagcgttt ccgcctttgc gccaattgtg
    61921 aatccgtgca gcgtcccgtg gggaatcaaa gcgtttagcc gctatttagg tgaggacaaa
    61981 aatgcatggc tggaatggga cagttgcgcg ctgatgtatg ccagtaacgc gcaggatgcg
    62041 atcccgacgc ttatcgatca gggcgataat gatcagtttc ttgctgacca actgcaaccc
    62101 gcagttctgg cagaagccgc gcgccagaaa gcatggccga tgacgctgcg tattcagccg
    62161 ggatatgatc acagttacta cttcatcgcc tcttttatag aggatcacct gcgcttccat
    62221 gcgcagtatt tactgaagtg aaagtccgcc cggttcgccg ggcatcttct catcagaagc
    62281 gataatccac tgccataaag taacgacgtc cgtcttcgtt atagctgtag tcgtcacgac
    62341 tgagatcctt gtcgccaagg ttcagcacgc ctgcgcgcag tttgacgtct ttagtcacct
    62401 gccaggccgc gccggtattc cagatggtat aaccgcccgg tgttttagcc gtcgcgctgt
    62461 cggctcgttt ctgcccggta tagtgaccag aaacatagaa tgaccagtct tccagcgcca
    62521 gcggtttcca gtccagcgta ccgttagcgg tatggaacgg cagatcggat agcggtttgt
    62581 tttcgccgtt gctgacatca cgaccatcgt tataggtgta gttgagtgac agtttccatt
    62641 catcgttgaa cggaattttc agttcggttt ccacgccctg aatacgtgct ttgttaacgt
    62701 tgtagtagct aaataccggc acaccgttcg ctgtaaaacc aacaaagttt tggtagcccg
    62761 gtgcagcatt gacgtcagac gtacggctga tgctgatacg atctttcaca tcgttacgga
    62821 aaacggtaac gctggattca accccttcca gccagccttc ttcacccatg tagtaaagcc
    62881 ccagctccca actttcgctg gtttctggtt tcagatccgg gctacccaca atcttacatg
    62941 cgccacggca ggaattgctc gtccagtcag ggctaagttg caacagagaa ggcgctttaa
    63001 atgccgtcgc ccagccccct ttcaccgtta cggtgtcggt ggcgttataa accaggtagg
    63061 cacgcggact ccagtgttca ccgtaggttt catgatcgtc catacgtacg ccggtcgtca
    63121 gcgccagcgg ctcgaagatt cgccattcat cttccacaaa cagcgcgtac tggctggcag
    63181 acgttttgga gctggttccc ccggtcaggt tcaccgcatc gctaagtttg tcgtgacgcc
    63241 attcaccgcc aaccgtgaga aactgattaa tcgccgtcag cggcaacgtg tatttgccgt
    63301 cgaccgtatt gctttcggaa gttatcgggc tgctgttgcc agggtttttg ttctcgactt
    63361 tctcaccgta gtatttcagt tcgctggtgc cgtaatccca acgcccatta tggctgacgg
    63421 agtagttctg gcgttccagg cggtttttgt ccagcgagtc ggaatcacga tcctgacggt
    63481 cgaaaccgta tccggcagta aaatcgtgat tttgattcgg tgtccaggca aattcgacat
    63541 tgccgtcgcg gctggagaat ccttcaatac gcggcgtttc tccggtatcg gtggtcgttg
    63601 agttttgcgg gtcatccttt tcacgttttg ccaggctgcc gtaagctttc attcccagca
    63661 caccatcaat taatggtcca ctggataaag aactgaccgt tataggtatc accgcgatcg
    63721 cgatgttcct gaatggtggt atcgacggta acggtacccg accatttctg accgattttt
    63781 ttggtgatga tattcactac accgccgagc gcatcggaac cgtacagcga cgacatcggg
    63841 ccacggacca cttcaatacg ttcgatggaa tcgaccggga tccagttcag atcgaaatca
    63901 ttgtggcgga agacggcatt gcgggagtta acgcgtttac cgtcgacaag aatcagggtg
    63961 tagctgctgt ccagaccacg aatactaacg cccttacggt tatccccttc gttcgtcagt
    64021 tgtacgccag gcacttcttt gaggacatcc ttcagattct gtaccggttt tcgctgcagg
    64081 tcttcctggg taatgacgct gatactggcg ggtgcatctt taaggttttg ttcaacggaa
    64141 gatgcagtga caaccatcgt ttcgccatcg tcatcgaccg ctaacacagg ccatgcacaa
    64201 gaaatagcgg acaaacacag cccgacccgt acgaaagggt tcaacctaaa cattccatat
    64261 ctccatgagg taactacgaa aataaaatgg gttatcgctc acatcttctt cacgtcccct
    64321 tgcgtacggc agcatcgcgg tggacttatt tgtatgcagg tgatcatccc agcaagccgg
    64381 accagcctct gatttgtcag ccttttttga ttgcggcgta acgataatgc aaacgataac
    64441 aattatcaat ccaaatgtta aattttatat ccgcatgatt tgtcagggaa atggaattga
    64501 aaaagccctc tcagttgaga gggcttagca aggaagggag aaacttattt cttatcgttc
    64561 tgcgggaact tcatttcgct gtagcgtacg aagtgggttc ctttaatcag cttgtagccg
    64621 aaccaaataa tcaggaacag cgggatacca atatacgttg ccgctacgcc gccccagtca
    64681 atggtatctt tcaggaatgc ttcgtagttc tggcccaaag tgataatcag acacagaatg
    64741 aatgcgaaga tcggacccag tgggaagaaa cctgaacggt acggcagatc gttaatgtcg
    64801 tgtccctgca atacgtaacc gcgacggaag cggtagtggc taatggcaat ccccagccag
    64861 gcgataaaac cggtcatccc ggaggtgttc agcagccaca ggtataccgt ctggttgcca
    64921 aacatggagg tcaggaagca cagaccggca atcaccgtcg tcgcatacag cgcattacgc
    64981 ggcacgccac cacgcgacag tttagcgaaa atgcgcggcg ctttaccgtc acacgccagg
    65041 gtgtacagca tacgagtaga cgcatacata ccggagttac cggctgacag caccgccgtc
    65101 agaataactg cgttcatcac cgccgccgca gagagcagac ccgcgtgctg gaacaccagg
    65161 gtgaacggac taacgctgat gtctttaaca tcgttacgca gcaggctcgg atcggtgtac
    65221 ggaataatca ggctgataat caggatcgcg aacacataga acaacaggat tcgccagaac
    65281 acctgacgta ccgcgcgtgg aatgtttttc gccggatctt cggactcgcc tgcagcaata
    65341 ccgatcagct cggttccctg gaaagagaag ccgacaatca tagctacgcc gatcatcgcc
    65401 gcaaaaccac cagcaaacgg cgcttcgccg attgtccagt tgctccagcc cgcaggctgc
    65461 gcgcctttga agataccgat aatcatcagc acgccaacga tgataaagac aataactgtc
    65521 gtgactttga tcagtgagaa ccagtattcc gcttcaccaa agccgcgaac agagatgtag
    65581 ttcagcagga agataacgcc gaggaacaac gcactccaga tccagcccgg tgtatccggg
    65641 aaccaccagc tcatgaccag ctgagctgca accaggtcaa cggcaatagt caccgcccag
    65701 ttgtaccagt agttccagcc cagcgcgaag ccaaagcctt cttcaacata gttctgaccg
    65761 taagtggcaa acgaaccgga aaccggcata tatgcagcca gttcaccgag actggtcatc
    65821 aggaagtaaa ccatcaggcc aatcaacata tacgagagca atgccccgcc cggacctgcc
    65881 tgagaaatcg ttgcgccaga ggcaacaaaa agacctgtac cgatggaacc gccaatggca
    65941 atcatcgtca ggtgacgcgc ctttaattca cggcgtaagc ccggcgcttc tgtggtttta
    66001 gtttcggaaa ccatacaaaa atgctatcca tctaaaaaat gaggcgcgat tgtagcagac
    66061 gattggcaat ccttccggca gaaatacacg gttataagac accttcatga tcgcccaggg
    66121 attataagta aagcagccaa tctcttttct ggcgaatgca tgctgaaggc gctgcgtcac
    66181 gaaatcgaca cagcaccagc gtgttcttgt acagcaactt aacgcggcac atttgcggga
    66241 tcgcaatagt ccagaaagcg ccgtagcgca ttggaaaggt gtttttgccg atgatgtata
    66301 cgccacaacg tacgcatcag gcgcggcaga gggaccgcaa cttcacttaa tgtgcctgcc
    66361 tgcaattggt cttcaatcac ccgtcgcgac aggcaactaa ttcccaaccc atgacgcacc
    66421 gcatgtttga ttgcctcgga gttacctaat tccatcgcca tctcaaactt cggtaaatgt
    66481 gacagcaaca gataatcgac aatctcccgc gtgccggagc cgcgttcacg caggatccac
    66541 ggcgcagcgg ccagctgttc taaggtgacc ggaccacggg ccaacggcga agtcggcgcg
    66601 gcgaaaacca ccagctcgtc ttccagccac ggttcagaaa tgatttcagt gctgtggcac
    66661 ggtccttcaa taaagccaat atcaacgcgg aaatccagca ccgcttgcat cacgtcctgg
    66721 ctgttcccaa cgctaagttc aatcggcaac tgcggataat catggcgata acgggcgata
    66781 actgcaggca gaatgtagtt accgatggta ctactggcat agatacgaat cgcgccgttg
    66841 tcttcgcgaa acagttgttc gatttcaacc gcctgttcaa gcaatgccaa cgcacgcgga
    66901 tagagcagcc gcccgtgttc attaacaacc agtcttttcc ccacgcgatc aaacagttgc
    66961 acgccaagtt gcccttccag gtcggtcaag gctgcgctca ctgctgattg cgacaacgcc
    67021 agcatcaccg acgcctgggt ggttgatcca cttttcaata cttctgcaaa aacttccaac
    67081 tgccggaggg tgatgtgcat agtcgcttac cacttataaa gattaattat aaatatataa
    67141 tcaattttat ttttaaacca gttagtcgta accttatgcc cggttaaagg agagggttat
    67201 gacgaatatc accttacaga aacaacatcg tacactgtgg cattttattc cggggttagc
    67261 cctgagtgca gttatcaccg gggtcgccct gtggggtggt tccattcccg cggttgcggg
    67321 tgccgggttt agtgccctca ccctcgcaat cttgttgggg atggttttag gcaacaccat
    67381 ctatccgcac atctggaaaa gctgtgacgg tggtgtgctg tttgccaagc aatatttatt
    67441 acgtctgggt atcattcttt atggcttccg tctgacgttc tcgcaaattg ccgatgtcgg
    67501 tatcagtggg atcatcattg acgtgttgac gctgtccagt accttcctgc tggcttgctt
    67561 cctggggcag aaagtgtttg gtctggataa gcacaccagc tggttgatcg gtgccggtag
    67621 cagtatctgt ggtgctgccg cggtactggc gactgagccg gtagtgaaag cggaagccag
    67681 taaagtaacc gtggctgttg caaccgttgt tatcttcggg accgtcgcga ttttcctcta
    67741 cccggcgata tatccgctga tgtctcaatg gtttagtccg gaaactttcg gtatctacat
    67801 tggttctact gtgcacgaag tggcgcaggt ggtggcggca ggtcatgcca tcagcccgga
    67861 tgcggaaaac gcagcagtta tttccaaaat gctgcgcgtg atgatgctgg ctccgttcct
    67921 catcctgctg gcggcgcgtg ttaaacagct ttctggggcg aacagcggcg agaaaagcaa
    67981 aatcactatt ccgtggtttg ccatcttgtt catcgtagtt gccatcttta actcgttcca
    68041 cctgttaccg cagagcgtgg tgaacatgct ggtaacgctg gataccttcc tgctggcaat
    68101 ggcgatggcg gcgctgggtc tgaccactca cgtcagcgcg ctgaaaaaag ctggggcgaa
    68161 acctctgctg atggcactgg tactgtttgc ctggctgatt gttggtggtg gtgcgattaa
    68221 ctatgtgatt caaagcgtca tcgcataaac cactacatct tcctcctgtt aacccgctat
    68281 cattaccgtt ttcctccagc gggtttaaca ggagtcctcg catgaaatac attggagcgc
    68341 acgttagtgc tgctggcggt ctggcaaatg ccgcaattcg cgccgccgaa atcgacgcaa
    68401 ccgcgtttgc cttgttcacc aaaaaccaac gtcagtggcg tgccgcaccg ctcacgacgc
    68461 aaaccatcga tgaattcaaa gccgcctgtg aaaaatatca ctacacatcg gcgcaaattc
    68521 ttccccacga cagttatctg attaacctcg gacatccggt cactgaagct ctggaaaaat
    68581 cgcgcgatgc ctttatagat gaaatgcagc gttgcgaaca gctggggctt tctttgctca
    68641 acttccaccc tggcagccat ctgatgcaga tttcagaaga ggattgcctt gcgcgtattg
    68701 ccgaatccat caacattgcg ctggataaaa ctcaaggtgt gaccgcggtg attgaaaaca
    68761 ccgccggtca gggcagtaac ttagggttta aattcgaaca tctcgcggcg attatcgacg
    68821 gcgtggaaga taaatcccgc gtcggcgtct gcattgatac ctgccatgct ttcgctgccg
    68881 ggtatgattt gcgtactcca gccgaatgcg agaaaacatt cgcggatttt gcccgtactg
    68941 tcggctttaa atatctgcgc gggatgcacc ttaacgatgc gaaaagcacc tttggcagcc
    69001 gcgttgaccg ccatcatagt ctcggtgaag gcaatatcgg tcatgatgcg ttccgctgga
    69061 tcatgcagga cgaccgtttc gacggcattc cgctgatcct cgaaaccatc aacccggata
    69121 tctgggcaga agagatcgcc tggctgaaag cgcaacaaac tgaaaaagcg gtagcctgaa
    69181 gatgaataac cgggaaaagg agatccttgc aattttacgg cgtaacccgc tgattcagca
    69241 gaacgaaatt gcggacatgc tgcaaatcag ccgttcgcgc gttgcggcgc atattatgga
    69301 tttaatgcgc aaaggcctga ttaaaggcaa aggttacatt ctcaccgagc aggaatactg
    69361 cgtagtggtg gggacaatca atatggatat tcgcgggatg gcggatatcc gttacccgca
    69421 atcggcttct catcccggta caattcattg ctcagcgggc ggcgtgggac gcaacatcgc
    69481 ccacaatctg gcgctgttag gccgcgacgt ccatttgctt tcagtgattg gcgatgactt
    69541 ttatggcgaa atgctcctgg aagaaacgcg ccgtgccggc gtgaatgtct ccggctgcgt
    69601 tcgtttgcat ggtcaaagca catcgacgta tctggcaatt gccaatcgag acgatcaaac
    69661 cgtgctggcg attaacgata cccatctgct ggaacagttg acaccgcaac tactgaacgg
    69721 gtcgcgcgat ttacttcgtc atgcgggcgt ggtactggct gattgcaacc tgacagccga
    69781 ggcgctggaa tgggtcttta ccctcgctga tgagatcccg gtgtttgtcg ataccgtttc
    69841 agaattcaaa gcgggcaaaa tcaaacactg gctggcgcat attcacaccc tgaaacccac
    69901 tttaccggag ctggaaattt tatggggaca ggcgatcacc agcgatgctg accgtaatgc
    69961 tgcagtgaat gcgttgcatc agcaaggtgt tcagcaactg tttgtttatt tgcccgatga
    70021 gtcagtttat tgcagcgaaa aggatggaga acaatttttg ctgaccgcac cagcgcatac
    70081 gacagtagac agttttggtg ctgacgatgg ttttatggcg ggcctggtat atagctttct
    70141 ggaaggaaac aatttccgtg acagcgcccg ttttgcgatg gcctgcgcgg caatttcacg
    70201 cgccagcggc agcttaaata accctacctt gtctgccgat aacgcgcttt cattaatgcc
    70261 aatggtgtaa caatgttgcc ggattcgacg cttgaccgca tattatccgg ctcataaatc
    70321 gtgcactacg ctaagccaat aaagaatcct gctatggtgg cactcatcag gttagaaagt
    70381 gtcgccgccg ccagcgcgcg taaaccaagt tgggcgattt ccggcgcacg gtgtggcgca
    70441 accgcagaaa acgcccccac caccaccccg atagaaccaa agttagcgaa accgcacaac
    70501 gcgaaggaaa taatcgccac ggttttagca tccagagtgc cagccgtttg cagatagggt
    70561 gagaaattga gataagcgac aaattcattt attgccagtt tctgtccaat caaactcccg
    70621 gtaagattcg catcagtcca gtcaacaccc attacccacg ccagtggggc cagcaggtaa
    70681 cctaaaatgg actccagcga ggcatgttca aaaccaaacc agccgccaac gccgccgata
    70741 ataccgttaa ttaacgcaat gatggcaaca aataccatca ccactgttgc cacacctgcg
    70801 gcgattttca gaccggtcat tgcccctgtc gcagcggctt caataatgct ttttggcggt
    70861 gtttcggtga aagagaggtt attaaaagaa acctgcgaag attccgtagc cgggcttaac
    70921 aggcgggcaa acaagattcc gcctgggatc gccattaacg atgccgccag caaatattcc
    70981 acaggtacgc ccagtgcggc gtaaccaatc attgtcgaac cagcaatcga ggccatgccg
    71041 ctacaaatcg ctgtaaataa ttcattgcga ttcagccgat cgataaaggg cttcacaatc
    71101 gccggaattt cgttttgccc gaggaaaatg gtagtgaccg cgacgaatga ctcgatcttg
    71161 ctgatattta acgctttctg gaagataccg ccgagaatgc gaattaagat ccccatcaca
    71221 ccgatgtagt agagaatact caccagtgct gtaacgaaga taattgccgg taatactctg
    71281 aaaccaaaga taaatcctgc gccatcaaac agcgtgtcca ttttcggccc gaccagcgaa
    71341 ccgaagataa atgcgctacc cgcgtcgctg tacgccatca ctttatgcac gccaaaagcg
    71401 actttttcag cgacccaacg ccctggcggt aaccaaagca taatcccgcc aatcacaacc
    71461 tgtaacacta acgccgcgcc aacggtacgc aggctgatct tcttcttgtt tactgacagt
    71521 aaaaacgcaa tcgtcagcaa taccaccatt cccagaacac ttctcatgac atccatagtt
    71581 attatctctt catgtcatat tgggcgacgg ctttctgccc ctcagaaata gccgggcctg
    71641 catcttgcga tactgacccg gcttgttatc aacacttaat gggttttgat gtagccgcgc
    71701 acgcactctt cgactaagcc ccagaaccag tcggtatcaa tagtgatgcc gactttggta
    71761 ttagcgggct tgccaagaac gcccagctcg tcgcagacgg tacgcccata gcaagggcca
    71821 ctgttgacgt ccacttcgac gtacatctcc tgggttttaa tgccatcagg gttaatcaga
    71881 taaccaatgc aggtggcgtc gtgcaccggg ccgccagcca ggccgtagtt ttcgaactgg
    71941 gttttgagag tgaagttcat gatgtcgctg aacagctctc cggcagggcc gcctgccctt
    72001 tccattcgtg caatcacgtc cggggtgcaa acggtctggt tggtgagatc gaggcccatc
    72061 atcactaatg gaacgccaga ggtgaacacc acgcgtgcgg cttccgggtc agcaaagatg
    72121 ttgaactcgg cagatggcgt gaagttacct gtaccgtaag cgccgcccat tagcacaatt
    72181 tcacggattt taggcaggat tgcgggttgc atacgcattg ccaccgcgat atttgaaagc
    72241 ggaccaaccg gcaccagagt gatatcgcca tcgcttgcca tcagggtatc aatgatgtat
    72301 ttcaccgcgt gggtactttc tgcctggcgg gtcagcggct cgaataccgg gccatccagt
    72361 ccggtttcac cgtgaatatt atcggcaacg atttgttgac gcataatggg ttgcggcatc
    72421 cccgcataaa ccggaacatt aatctccagt ttctggcaaa cattcaggcc attaattaat
    72481 gttttatcaa gcgtctgatt acccgcaaca atggtgatac ctaataaatc aatcgccgga
    72541 tgtttcgccg ccatcattat agcaatagca tcatcatgac ccggatcaca atccagaata
    72601 atttttctct tttccattgt ttatttcctc tgtttccagt tgcgttattt tttctacagc
    72661 aaagaaattt cgatcgggcc gatgattgaa tcttaacaac agcgtacgta tgctaaatat
    72721 gagaaatctc atagcggata aacatcgtga aagaaatcca caataatgat cttaagcagc
    72781 aattgatgag tgaatccgcg tttaaggatt gctttttaac ggatgtttca gccgatacgc
    72841 ggctgtttca ttttttagcg cgtgactaca ttgtgcagga agggcaacag ccgtcctggc
    72901 tgttttacct gacgcgaggc cgcgccaggc tttacgccac gctagctaat ggtcgcgtgt
    72961 cgctgatcga tttctttgcc gcgccctgtt ttattggcga gattgagtta atcgataaag
    73021 accatgaacc gcgtgcggtg caggctattg aagagtgttg gtgccttgcg ctccctatga
    73081 aacattaccg tccgctgtta ttaaacgaca ccctattttt acgaaaactt tgcgtcacct
    73141 taagtcataa aaattatcgt aatattgttt ctttaactca gaatcaatca tttccgttag
    73201 tgaatcgcct ggcagcattt atattactct cacaagaagg tgatctttat cacgaaaaac
    73261 atacccaggc agcagagtat ttaggcgttt cttatcgaca tcttttatat gttctcgcgc
    73321 aattcattca cgacggttta ttaattaaaa gcaagaaagg atatctaatt aaaaacagaa
    73381 agcagttgtc agggctggcg ctggagatgg acccggagaa taaattctcc gggatgatgc
    73441 agtaaaaatt atttgcaata gtgcgattgc cggatgcaac gcttaataag cgagtacatc
    73501 cggcaatgaa actcaagcta aaccaataaa gaacccggca atggtcgcgc tcatcaggtt
    73561 ggaaagcgtt gctgctgcca gcgcccgtaa accaagctgg gcgatttccg gagcacgttt
    73621 tggcgaaata gccgaaaatg ccccaacgac aacaccgata gaaccaaagt tagcaaaacc
    73681 acaaagcgca aaggagataa tcgcaatggt tttcacttcc agcgtgccgc ccgtttgcag
    73741 gtatggggag aaactcaggt aagcgacgaa ttcgttaatc gccagtttct gcccaatcag
    73801 gctacccgca aggttggcat cactccagtc cacacccatg atccacgcca gcggtgccag
    73861 cacatagcca aaaatacttt ccagagaggc attggcaaaa ccaaaccagc cgccaattcc
    73921 gccaataatg ccattaatca gcgcaataat tgcgacaaac gccatgacca ccgtcgccac
    73981 accggcggcg attttcagcc cggtcatcgc accgctcgcc gctgcttcga taaagctttt
    74041 tggcggcatt tcgctgaacg acagattttc aaacgtgacc tgcgaaggct cggttgctgg
    74101 gctaagaata cgtgcaaaca aaataccgcc tgggatagcc atcagcgatg ccgctaacag
    74161 gtagtcaatc ggcacgccca ttccggcgta accaatcatc atcgaaccgg caatggatgc
    74221 catcccgcta caaattgcgg taaacagctc gttgcgattc atgcgatcga taaacggttt
    74281 aacgatcgcc gggatctcgt tctgcccgag gaaaatagtg gtaaccgcaa caaaagactc
    74341 gattttgctg atgttaagag ctttctggaa aatgctgcca agaatacgaa ttagcagtcc
    74401 cattacgcca atgtagtaca gcaggctgat aagcgcagtg acgaagataa tcgccggaag
    74461 tacgcgaaag gcgaagataa agcctgcacc gtcgaacaag acatccattt tcggcccgac
    74521 cagcgaacca aaaatgaagg cgctaccggc atcactgtaa gacatcactt tatgaacgcc
    74581 taatgccgcc tgttctactg cccattttcc cggcggaaaa tagagcatga tgccaccaat
    74641 agcgatttgc agcagcagtg cggctccaac cgtgcgcaaa ctgatgctct ttttatttac
    74701 cgacaacaga aatgctattg ccagtaacac caccatcccc acaacacttc tcattatatc
    74761 cataatgatt ttcccttcat gccagtaaac ccggcgtcag cgccaggttt cggtatgtat
    74821 gatcagtacg ggcgacggct ttctgcccgt cagaaattaa cccgcgagac gctggtattc
    74881 tttggcaatt tcgctcgcca gaatggcgtt gttgaacacc agttggatgt tggatttcag
    74941 gctgtcaccg ccggtcagtt cagcaacacg cgccagcagg aacggtgtac tttctttacc
    75001 aataacgccc tgctcttcgg cttcggcgac ggcttgatct atggctgcat tgatagattc
    75061 ctccggcatc gcaaactgtt ccgggatcgg attcgccact accagaccac cgttcaaacc
    75121 ggtttgccat ttcactgcca ttgcacgagc gatttccgtg gcactatcaa ggcgaatact
    75181 gacttcaaat gggctggtgc ggcagaaaaa cgcaggcagc gctttagtct gatagccaat
    75241 taacggcaca ccgaaggttt ctaaatactc agtggttaat cctaaatcga gaatagattt
    75301 cgccccggca caaacaacgg tgacattagt atttgccagt tcttgcaaat cggcagaaat
    75361 atcgaaggta tgttccgccc cgcgatgaac accaccaatt ccgccggtgg caaatacttt
    75421 aattccggca agcgccgcaa taatcatcgt tgaagccaca gtggttgcgc cattttttcc
    75481 ggcggcaaca acaaaaggta aatcgcgacg actaactttg gtcacgttat gcccttcacg
    75541 acccagtaat tcaatttctt ctttgcttaa acccactttc atcacgccgc caataatggc
    75601 gatagtggca ggtactgcgc cctgtttacg aatagtttct tctacttcaa ttgcggtctg
    75661 ggcattttgt gggaacggca tcccgtgaga aataatggtc gattccagcg ccacaaccgg
    75721 ttttttgttt tttaaagcgt cctgcacttc cggggaaatt tgtaataatt caggggaaat
    75781 ttttaattca gacattctgc gttctccact aacgatataa cgttggcaat cgataaatcg
    75841 gggttattgg tgtattcaca ggagagcgcc attgacgaac atccctgtgc gaaacgaaca
    75901 gattcggcaa acggcattcc gtctacccaa cacgaagcaa gtcccgccat catggcatcg
    75961 cccgctccgg taacattaat aacattggtt ttgatcggcg cagaccaacc actttcaccg
    76021 ctgatatcac tgtaataaac gccgtcgccg cccatgctca ataccagtcg gttcaggcca
    76081 tgttgatgga accaggcggc aacctttgcc acatcttcac gccctgacag cgcaatccca
    76141 ctcagggttt ccgcttcaag gcggtttggc ttgagagtgt ggatctgatt tagacggtcg
    76201 cgcactttga cacatttcca cgctgaaacc ggatcgacaa atacgggtac gttggcagca
    76261 ttatccagaa tccatgccag tgcctcttca ctgatattac agtccgcgac aatgaccttt
    76321 gccctctgaa taaattcacg gtgctgtgcg agatattcag ctgtaatagc gttgctaata
    76381 ttcatgtcat ttatagcaac cagcatttca ccggtattat cgagtaatga taaataactc
    76441 gacgtatttt ctcccggcac aatcaggcat ttatcgacat aaacgccaga ttgattggtt
    76501 tgcgttagca gcgattgacc ataaaaatca ctacctactg cgctcagtaa ccaggctttg
    76561 ttacccagca acgccaggtt ttgtgcaata tttcgcccta ctccaccagg tgtaaatttt
    76621 attttacctg gatttgaatc cgcataattt aatgattcat gtgaatatcc ggcgacatca
    76681 atattcgccg aacctataat tacgacataa tccttttcgc gcatagcacg tcctctggta
    76741 aaaaattacc aaccctgtca atccacaagg atatagatac tgcaaagacg gtttaaacat
    76801 gtgttcatat ttaaacatgt gctcatagta tgcatgtttt acgtaaagta aattgccgcc
    76861 agagaggcaa agcgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta
    76921 caaaagcgag gaatttgtga cgggatgcac agaacatgaa cactaaaaaa gggcagaaaa
    76981 gaatctgccc ttgaggaaat aaccttagtg tttgatgaac gtttcaggtt tctactgaaa
    77041 gataaaacca tttaaattca taaaattaca ttcacaaacc ggtcattctc tttgcgctca
    77101 tccaatgata cagggcagta aatttctgcc ctgtaacaca ccttttatta cgcggctttc
    77161 gctactgcgt ccacttccgg acgtttcagg aaggcatagg ccaaacccgc caccagcgta
    77221 ccggcaataa ttgctaccag gtaacccagt accggcgtaa tagcgccagg gatcagcaga
    77281 acaaacagac caccgtgcgg tgccatcagt ttcgcaccaa tcgccattga gattgcgcca
    77341 gtcagcgccc cacccacgat acagcacggc agcacacgca tcggatcacg agcagcaaac
    77401 ggaattgcac cttccgaaat aaagcacaga ccaagaacca gagcggcttt gccaccttcc
    77461 tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg ccagacccat tgccagcggt
    77521 ggcaccatac ctgccgccat aatcgccgcc atcgggccat aggtttgagt actcagcaga
    77581 cccacaccga atgcgtacgc tgctttattt accggaccgc ccatgtcagt acacatcatg
    77641 ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc ccatggtctg cagccagtga
    77701 gtcaaccctt cgagaatgcc agcaaccggt ttaccgatca ggtagatcat cgccagaccg
    77761 accaccagac tggaaattag cggaatgatc aggatcggtt tcagcgcctc catactctgt
    77821 ggcagtttca gttgcgtact gattaacttc gcaatgtaac cagccaggaa gcccgcaata
    77881 ataccgccaa tgaagccaga accggtgctg accgccagca taccgccaat cagacccggc
    77941 gtgagacccg gacgatcggc aatggagaag gcaatgtaac ctgccagtac cggcaccatc
    78001 agcgcaaagg ctgaaccacc accaatctgc atcagcgccg ctgccaacgt acccggctct
    78061 ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac acagaccacc tgcaacgacc
    78121 atcggcagca tgtaagagac acccgtcagc aagtgacggt atgcgcctgc actctctttc
    78181 ttaccttcag aggtcgccgt ttgagctttg cccgccggtt catacggcgt tgcttcagca
    78241 accgctttat ccagttcctg cgcggttttc ttcagcgcca gaccggtaga ggtacgatac
    78301 atcggtttac cagcaaattt cgccagatcc acttcgatat ctgccgccac aatcaccaga
    78361 tccgctgcgg ctacttcttc gggggtgatt gcattacccg caccaacaga accacgggtt
    78421 tcaactttca cccaccagcc acgttttttc gcttcggttt caatggcttc agccgccata
    78481 aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa ctacgcgttt cggaccgctg
    78541 gcggcaaccg gtgctgtcgc agcgaccggc gcagtgtaag gtttcgcatg gcctttggct
    78601 tcactcagga acagctcagg gtgcgcaact gcccgggaaa tatcgcccag ccagacattt
    78661 ttaccgttca gcgcgctgtc attcgggatg gaatcaccga gaacaatcgc catttctgcg
    78721 tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg ccgccgcgcc cagcagggtc
    78781 ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa taatcagcag cgttttcatt
    78841 atgcctctcc tgctgtcagt taaaaggttg taagtcgacg cgcgccatca ttgcggccaa
    78901 ctgcggacga tcggtaatac ccacattgct ttgacttacc gccagggctg caacagcagt
    78961 cgccagacgc agtgtgtgtt cactggattc acgcattagc aagccataaa tcaggccacc
    79021 aaccatagaa tcccctgcgc caacggtgct tacgacatcg actgacggtg gtttggcgat
    79081 ccattcgccg gaggcattaa cccaaagcgc gccttcggca cccagtgaaa taacgacatg
    79141 cgcaatacct tgttcacgca gcgcatgcgc agcttcaatc acatctttca tttcaggcag
    79201 tttacggcct gcccagattt ccagctcgcg gcggttaggt ttcaccagcc acggtgccgc
    79261 tttcaaacct gctactaacg cttcacggct actatcaaag ataatgcaag ggcactgact
    79321 acgcaggcga gtcatccagt cggtgaacgc ttccgggctg acgcctgacg gtaagcttcc
    79381 gctgacacag accatatcga actgaccgag ccagctcaga gaatcagtca caaagcgttc
    79441 ccagtcggcg ggagtgactt caaaacccga gaagttgaag tcggtcactt cgccgtcttt
    79501 ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca acctggaaac ggttggcaat
    79561 gcccagctcg ctgaacagtt gctgaaaacc atcctgattg tctttaccga ggaagccgcc
    79621 aacggtgaca tcaattccca ggtcttttaa tactttggcc acgttgatgc ctttacccgc
    79681 cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg cgttcaattt ccgggcagaa
    79741 accaacaagg tcataagccg gattaagggt gatagtagca acacgtctgc tcattatgcg
    79801 ccctccccaa gaccagcagc gatagcgtcg ccgattgctt tcagcgcctg ttcagcatct
    79861 gcaccctggg cggtaaagcg taggcgatga cctttcttaa cgccaagtgc cacaactttc
    79921 atcagactac gtccgtttgc cggtttgccg gtgccatcaa ggtttgtcac ggtaatatca
    79981 ctgttaaatt gtttaatggt gttgaccagc atggtacctg gacgagcatg caggccgtgt
    80041 tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg tcggcgcatc atcgctggtc
    80101 agcagcgcca gcaacgttgc cgcatccgct ttcagcaagc ggtcagcttt attgtcgagc
    80161 aacaaatcag cgagacgctt aagaaccgca atgggctgat cgtcattcat cgccacactc
    80221 accagcatgg ctgccgtttc gccgtccaca tcaaaagcat ttgccgcacg gcttacagca
    80281 atcgcgctac gcaaattgcc ttcggcgcta tcgctcagcc agatgccctg tccgaggttc
    80341 agcggttgtt cattgatggc tttggtgacg aaagtggcgt caactgcccc cgcttctttc
    80401 agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc tggcgacgat atccagcgtc
    80461 agcatttcgt tgtcgagctt cagctgctca ctctgctttt cgcccatcag caatgcgcga
    80521 agttcttctg ctgttgttgc tgacttcagt tgttcagcaa cggaatcatc gctcagtacg
    80581 tgggtcagct ggcgtagcag gcccagatgc tcatccgagc tggcagcaat accgatcgct
    80641 acgtacgcta cctgaccgtc accccaggtg acgccttccg ggaactgaaa tacctgaacg
    80701 ccggttttca gcacctgatc gcgggtgtcg gtagtgccgt gtggaatagc aataccattg
    80761 ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc cattgacgta gccttctgct
    80821 acattaccgg cctgcaccag cgccgcagcg acctggcgaa tcgcctcttc tttgtctccg
    80881 gccttttcgc ccggatggat gtcctgtacg gataactgga acatagttct cctctcttgc
    80941 tgaattgaaa cgattcagct tctatgagaa aaaaagcgcc aacctggctt agggttaaag
    81001 acaagatcgc gctgaaacgt ttcaagaaag catagtactt ctgtttcagc acgcaaggaa
    81061 agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca tttttcctaa
    81121 ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc agccaatcag
    81181 caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt ctgtttattt
    81241 tctgacaagc agcgtaaact ccgcgtcttc ctcttccaat gatcgaccag catgcataac
    81301 tcccccgcag tctccagcgc gaaatcgttt gacctgacct cgacggcgtt tttaatcgtt
    81361 gcctttctca ccggtattgc gggcgctctg caaaccccga cactcagtat ttttcttacc
    81421 gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag cgctgtcatt
    81481 gggattctgg tgagtcagtt tctcgccggg cgctctgata agcgcggcga tcgcaaatcg
    81541 ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc ctggaatcgc
    81601 aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc gaccgctaac
    81661 ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga ggcggtgatg
    81721 ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc cccgctggct
    81781 tatgccttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc ggtagcgttt
    81841 attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga gcttccgctg
    81901 gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgctgct gctgtttgtc
    81961 atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc gctatttatt
    82021 atcaacgaac tgcatcttcc tgagaaactg gccggtgtga tgatggggac cgccgccggg
    82081 ctggaaatcc cgaccatgtt gattgccgga tattttgcca aacgtctggg taagcgtttc
    82141 ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct gatggcgcat
    82201 tcacctgcca ttctgttggg cttgcagctg ctaaatgcta tttttattgg cattctgggc
    82261 ggcatcggga tgctctattt tcaggatttg atgcccggtc aggcaggttc agccaccacg
    82321 ctctatacca acacgtcgcg cgtgggctgg atcatcgcag gatctgtggc gggcatcgtc
    82381 gccgagatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat tatcgccact
    82441 ctgttttgct tactgcggat taaagatgtt taaggcgcag tcagcatttc cagatcgata
    82501 agccaggtca ttgcctgctg acgcgagtta ccgcacattt cagcgctggc ttgtaagcct
    82561 gcacagactt tcggccgcag tggcgaggtg aaaattttac agcgttgctg ttcatcaagc
    82621 tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg gctggaaatt
    82681 gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg ttctcttaag
    82741 gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag caaaagtgcg
    82801 aataccactt gcccgaaagg cccgtcgcga gtactttgtc gcgatatttt tgacattttc
    82861 gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat ggtactgaat
    82921 tacaacggca aactgctgct ggttaaggac attgatattc agtcgcccac tgcccgcggt
    82981 gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa agtagaagag
    83041 cgtttcaaag gagatgatat cgttgacacc gtgacgctga cccgccgtta cgttgacttc
    83101 tcctatgtcg atggcaacga atatgtcttt atggataaag aagactatac cccgtatacc
    83161 ttcaccaaag atcagattga agaagagttg ctgtttatgc cagagggcgg catgccggac
    83221 atgcaggtgc tgacctggga cggtcaactg ctggcgcttg agctgccgca gaccgttgat
    83281 ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat ctgccagcgc ccgtaacaaa
    83341 ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag cccgggcgaa
    83401 aaaattcgta tccatatcga agaacgccgt tatatggggc gtgctgacta acttcagccg
    83461 catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc aatcttctat
    83521 tttgcgcttc ctgaacacct tacgccctca attttcactc gttgattgat cgccctcaca
    83581 ctttatcgca ttaacaatcc agaccaattt caattgctgt catataactt tacactgtca
    83641 ttgttaatta atcgttacta agacgtgact cctatgaata caatcgcctc cgttacgctc
    83701 ccgcatcatg tacacgctcc acgctacgat cgccagcagt tgcaatcacg tatcgttcat
    83761 tttggctttg gagcctttca ccgcgcacat caggcgttac tgaccgatcg tgtgctgaat
    83821 gcccagggcg gtgactgggg gatctgtgaa atcagcttgt tcagcggtga tcaactgatg
    83881 agccagctcc gcgcacagaa ccatttatac accgtgctgg agaaaggggc ggacggcaat
    83941 caggcgataa ttgtcggtgc cgttcacgaa tgcctgaatg caaaactgga ttccttagcg
    84001 gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac gattaccgaa
    84061 aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagcaatcc gcggattatt
    84121 cacgatctac aaacccctga agaacctcac tccgcaccgg gtattctcgt cgaagcactg
    84181 aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga caatattccc
    84241 gacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg ttcgccagaa
    84301 ctcgccgggt ggataaaaga acacgtcagt tttccgggaa ccatggtcga ccgcattgtt
    84361 ccggctgcaa ccgacgaatc actggcagaa atcagccagc atctgggggt aaatgatccc
    84421 tgcgcgatta gctgcgaacc gtttatccag tgggtggtgg aagataactt cgtcgctggg
    84481 cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc atgggaagag
    84541 atgaaactgc gtatgcttaa tggcagccac tcttttctcg cttatctggg ttacctctca
    84601 ggattcgccc atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc cgccagaaca
    84661 ttaatgctgg atgagcaagc gccgacactg cggattaaag atgtcgattt aacacaatat
    84721 gcggataagt taattgcacg ttttgctaat ccggcgctga aacataagac ctggcaaatc
    84781 gcgatggatg gcagccagaa attaccgcaa cgcatgctgg caggtattcg catacatctg
    84841 gggcgcgaaa cggactggtc gttgctggca ttaggcgttg caggctggat gcgttacgtc
    84901 agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag cgataaaatt
    84961 cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtca ccgccctgct ttccctgcgt
    85021 gaagttttcg gtgatgatct gccagataac ccgcattttg tgcaggccat caaacaagcc
    85081 tggcaacaaa tcgcacaatt cggcgcgcat caggcgctat taaacaccct caaaatttaa
    85141 cgatttctgc ggttaaagcg gatgaagctc gccttcgtcc gctctcccct tctcttttct
    85201 gccgttttta gccaggatta acgctcagtt aacttatcag agtattgcgg agccgttgtg
    85261 accaggacca acctcatcac cggttttctc ggcagcggga aaactacgtc gattcttcat
    85321 ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga atttggggaa
    85381 gtcggaattg atggtgcttt gctcgtcgat agcgccgcat tgctgaaaga gatccccggc
    85441 ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac cttactgcgt
    85501 cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc gaaacagatc
    85561 ctcgatctgt taaccgcacc agtctatgaa ccgtggatag atctgcgcgc cacgttgtgc
    85621 attctcgatc cacgcctgct gctggacgaa aaaagcgcca gcaatgaaaa cttccgtgac
    85681 cagctggctg ccgcagacat cattgtcgcc aataaatccg accgtgcgac gcctgaaagc
    85741 gagcaagcgc tacagcgctg gtggcagcaa aatggtggcg atcggcaatt aattcacagt
    85801 gagcatggga aagttgacgg tcatcttctg gatttgccgc gtcgcaattt agccgagttg
    85861 cccgccagcg ccgcgcattc tcatcagcat gtcgtgagaa aagggttagc agcgttaagc
    85921 ctgccagagc atcaacgctg gcgtcgcagt ctgaacagtg gacaaggaca tcaggcctgc
    85981 ggctggatat tcgacgctga tacggtattc gacaccattg gcattctgga atgggcgcga
    86041 cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct ggtgcgaatc
    86101 aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc ggacagtcgt
    86161 attgagctaa tttccagcag cgaagctgac tggaatgcct tgcagagcgc gctgttgaag
    86221 cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata attagagaaa
    86281 atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc gcgctgtttc
    86341 tttcctggta tattcccgtt aatcatggat tctggttgcc gattgatgcg gatatttttt
    86401 atttctttaa tcagaaactg gtcgaaagta aggccttttt gtggctggtt gcattgacca
    86461 acaatcgcgc tttcgacggt tgttcactgc tggcgatggg aatgttgatg ctgagtttct
    86521 ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat tattggtctg gtcatgctat
    86581 taactgcagt ggtattaaac cagctgggtc aggcattaat tcctgtaaaa cgggccagcc
    86641 caacattgac ttttaccgat attaaccgcg tcagcgaact gctctctgtc cccacgaaag
    86701 atgcctcacg agatagcttt cccggcgatc acggcatgat gctgcttatt ttttcggcat
    86761 tcatgtggcg ttatttcggc aaagttgcag gtcttatcgc ccttattatt tttgtggttt
    86821 ttgcgtttcc cagagtaatg attggcgcac actggtttac tgacatcatt gtcggttcga
    86881 tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt gatcgattaa
    86941 tcaccttttt tgacaaatca ctaccgggaa aaaacaaaca tttccaaaac aaataactca
    87001 cagtaattaa catcatcagg tttattttta tagtgaggat aatcctgatg atgcgcaccg
    87061 tgctttcatc catcgaaagc aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt
    87121 tcactcaatc gtactttaat tgaccaatcc cgcttattaa ctttctgtat cactttttct
    87181 tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg
    87241 ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt
    87301 tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt
    87361 tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc
    87421 aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt
    87481 ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta
    87541 gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg
    87601 acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgt tatcgtctgg
    87661 gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca ttccgtgagc
    87721 aatttggctt agaacttccg cgttcgactt acgaacagca ggaaatgggt aaatctgttt
    87781 cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca acgggacgcc
    87841 atgtcggtat ttatatcggc aacaatcagt ttgtccatgc ttccaccagc agtggtgtta
    87901 ttatttccag catgaatgaa ccgtactgga agaagcgtta caacgaagca cgccgggttc
    87961 tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg
    88021 aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc
    88081 gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg
    88141 aaattaacgg aatcatgttc atacgcgctc ccaattctgg acgtaagctc ctgcttacct
    88201 gcattgttgc aggcgtgatg attgcgatac tggtgagctg ccttcagttt ttggtggcct
    88261 ggcataagca cgaagtcaaa tacgacacac tgattaccga cgtacaaaag tatctcgata
    88321 cctattttgc cgacctgaaa tccactactg accggcttca gccgctgacc ttagatacct
    88381 gccagcaggc taaccccgaa ctgaccgccc gcgcggcgtt tagcatgaat gtccgtacgt
    88441 ttgtgctggt gaaagataaa aaaacattct gttcatctgc gaccggtgag atggacattc
    88501 cactcaatga attgattccg gcgctcgaca ttaataaaaa tgtcgatatg gcgatcttac
    88561 ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc
    88621 tgaaaaatag cggcgtcttt gccgctctga atctcaacct gacgccttca ctcttttata
    88681 gttcacggca ggaagattac gatggcgtcg ccctcattat tggcaatact gcgctatcta
    88741 ccttttcttc acgtttgatg aacgttaacg aattaaccga catgccagtc cgtgaaacta
    88801 aaattgcggg cattcctctg accgttcggc tttatgcaga tgactggaca tggaacgatg
    88861 tgtggtacgc atttttactg ggcggcatga gtggaactgt cgttggcctg ctctgctatt
    88921 acctgatgag cgtacgtatg cgccccggca gagaaatcat gaccgccatc aagcgcgaac
    88981 aattttacgt ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta acgggcctgg
    89041 aagtactgct acgctggcgg catcctgtcg cgggagaaat tcccccggat gccttcatta
    89101 actttgccga atcgcaaaag atgattgtgc cgctgactca gcacctgttt gagttaattg
    89161 cccgcgatgc cgcagaatta gaaaaagtgc tgccggtagg cgtcaaattt ggtattaaca
    89221 ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt
    89281 ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag
    89341 agcaagaagc cacacaactc ttcgcctggc tgcactcggt cggcatagaa attgctattg
    89401 atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc
    89461 tgaaaattga ccgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac
    89521 ttgacgcggt gctgacgctg gcgaaacgcc tcaatatgct gacggttgct gaaggggtcg
    89581 aaacgccgga acaggcgcga tggctaagcg aacgcggcgt taatttcatg caaggctact
    89641 ggattagtcg cccgttaccg ctggacgatt ttgttcgctg gctgaagaaa ccgtatacgc
    89701 cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg
    89761 gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta
    89821 tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa ggaaagctat gcctttgccg
    89881 tgctgggcga accccggtac gcgtttaatt tcaaccattt tgattatgtg aaccccgccg
    89941 cgccaaaagg tgggcagata acgttgtcag ccctcggcac cttcgataat ttcaaccgct
    90001 atgcactgcg cggcaacccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga
    90061 cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagtgca cgctatgctg
    90121 acgattattc ctgggtggag gtcgctatta atccgcgcgc ccgttttcat gatggttcgc
    90181 ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgtccgaa ggcgtgccgc
    90241 aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta accgtgcgca
    90301 ttgagttagc taaacccggc aaagaagata tgttgagtct gttttcgctg ccggtatttc
    90361 cagaaaagta ctggaaggat cacaaactta gcgatccgct cgccacgcct ccgcttgcca
    90421 gtggtccgta ccgaattacg tcctggaaaa tggggcaaaa tattgtctat tcccgcgtga
    90481 aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc
    90541 gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcttttg
    90601 atttgcgtat ggaaaacgac gctaaaaact gggccacgcg ttataccggt aaaaatttcg
    90661 ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc
    90721 tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtccgcgaa gcgatcaccc
    90781 tcgcctttga ctttgaatgg atgaacaagg cgttgtttta caatgcctgg agtcgcacga
    90841 acagttattt tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg
    90901 tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacacaa atctaccagc
    90961 cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc
    91021 tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt taatgccaca acgggtcagc
    91081 cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc
    91141 agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa
    91201 tcaccaaccg catgcgcagt cgcgactatg acatgatgcc gcgcctatgg cgggcgatgc
    91261 cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat tccacttata
    91321 atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc
    91381 agggaaataa agaaaaatta ctgccgttag ggcgagcact ggatcgcgta ttaacgtgga
    91441 attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat
    91501 tctcccaacc cgctgtgcgc cctgtttaca gtctgggtat cgatacctgg tggtatgatg
    91561 ttaataaagc ggccaaactg ccgtcagcca gacaacaggg agagtagatg ggcgcttatc
    91621 tgattcgccg tctgttgctg gtgatcccaa cgttatgggc gattatcact atcaactttt
    91681 tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg
    91741 gtaatgccgg agtattaccc ggcgcaggcg gtgaaggcgt tcgcgccagc catgcgcaaa
    91801 cgggcgtcgg taatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag
    91861 tgatcgccga gatcactcat cgctacggtt tcgataagcc gatccacgaa cgttacttca
    91921 aaatgctctg ggactacatc cgctttgatt ttggcgatag cctgtttcgc agcgcctcgg
    91981 tgctgacgct gattaaagac agtctgccgg tttccatcac cctcggattg tggagcacgc
    92041 tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagcc
    92101 gctttgacgt ctggagtagc gcatttatca tcatcggcta cgccattccg gcctttttgt
    92161 ttgccatcct gctgattgtc ttcttcgcag gcggcagcta tttcgacctg ttccctctac
    92221 gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc
    92281 tgtggcatat cacgctgccg gtgctggcga cggtgattgg tggctttgcg gcgctgacta
    92341 tgctgacaaa aaactcattc cttgatgaag tacgcaagca atacgtggtg accgcgcgtg
    92401 cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc
    92461 tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga
    92521 ttgaggtgat gttttcactc aatggtcttg gcttactggg ctacgaagcg accgtctcgc
    92581 gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga
    92641 atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagatttt gagggacgtt
    92701 aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc ataaccgtcg
    92761 cggctactgg tcgttatgga ttttcctcgt tttgtttggt ttgagtttgt gttctgaact
    92821 tatcgccaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttgtt
    92881 gaaaaattac agtgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga
    92941 cccgtggctg aaacaacggc tggaaaataa cggctgggta ctgtgggcac cgattcgctt
    93001 tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca
    93061 aaactggctg ggaacggatg ccaacggcgg cgatgtgctg gcacgtattc tctatggcac
    93121 gcggatctcg gttctgtttg gcctgatgct gactctctgt tccagcgtga tgggcgtgct
    93181 ggcgggggcg ctacaaggct attacggcgg taaagtcgat ctctgggggc aacgctttat
    93241 tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca
    93301 gcctaacgtc tggtggctgc tggcaattac tgtcttgttt ggctggatga gtctggtcgg
    93361 cgtggtgcgg gcggagtttt tacgtacccg taatttcgac tacattcgtg cggcgcaggc
    93421 gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgcctaatg ccatggtcgc
    93481 gaccctcacc tttttaccgt ttattttatg tagttcgatc accaccctga cctcactcga
    93541 tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggtgaactgc tgttacaagg
    93601 gaaaaataac cttcaggctc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt
    93661 attgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc
    93721 ggtgtagcat gacgcaaact ctgttagcga ttgaagattt gtcggtgggt tttcgccatc
    93781 agcaaaccgt acgtacagta gtcaatgatg tttcactaca gattgaggct ggcgaaacgc
    93841 tggcgctggt gggtgagtca ggttcaggca aaagcgttac cgcgctgtca attttacgcc
    93901 tgctcccatc cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc
    93961 tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc gccatgattt
    94021 ttcaggagcc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag
    94081 tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc
    94141 ttgatcgcgt cggtatccgc caggcggcaa aacggctgac agattatccg catcagctct
    94201 ccggcggcga acgccagcgg gtgatgattg cgatggcact gttaacgcga ccggaattat
    94261 taattgccga tgaaccgacc accgcactgg acgtctctgt ccaggcgcag attttacagc
    94321 tgttgcgcga actgcaaggc gagctgaata tgggcatgct gtttattact cataacctca
    94381 gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc
    94441 aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca
    94501 acagtgaacc gtcaggcgac ccggtgccat tgccagaacc tgcctcaacg ttgctggatg
    94561 ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc
    94621 ataatgtggt ggtgaaaaac atcagtttta cgctacgagc gggtgaaaca ctgggtttag
    94681 tgggcgagtc cggttccggg aaaagcacga cgggactggc gctgctgcga ctgattaatt
    94741 ctcagggcag catcatcttt gacggtcagc cactgcaaaa tttaaatcgc cgccagctgt
    94801 tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctcg ctcaacccac
    94861 gactcaacgt tttgcagatt attgaggaag gcttacgggt tcaccagccg acgctttctg
    94921 ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa
    94981 cacgccaccg ttatccggcg gagttctctg gtggtcagcg acaacgtatt gcgattgcca
    95041 gggcattaat tcttaagccc tcgctgatca tacttgatga accgacatca tcactcgaca
    95101 aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg
    95161 cctatttgtt tatcagccat gatttgcacg ttgtccgcgc gttatgtcat caggttatcg
    95221 tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccacaccgc
    95281 agcaggagta tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt
    95341 gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg
    95401 tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact
    95461 tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg accatgcatt atcaccatcc
    95521 gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc
    95581 ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg cagtcgatgg
    95641 acgattgaga gttgtaatga agtcacttat tttccccggg aagcacattt acttcacaat
    95701 ttatttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    95761 gatgcctcac ctgcgtggcc tctatggctc tgatttaagt aatatctgcg ggcgtggtca
    95821 cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg
    95881 atgtgacttt ttacacaaat tgattttaca taaaataaac atatatcggg ggaatgatag
    95941 atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg tgcaatagat
    96001 caccgttttt tcggccgact ggcgtacaga cagaagagaa tggagctggt tgcgcagaat
    96061 gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag caatgcgcca
    96121 acaattgctc ctatgccaaa acggaaggtt cctgccagcg aagatgccgt tcccgccata
    96181 tggggaaact catcaagaat gaccgccatc gcgttggatg acaccatcga cacgcagccc
    96241 acaaacgccg caacgccaac caccagcgac caaaatccca gccccagcag cgcactgatg
    96301 accatccacg ctgccataat aaattgtatc cacaaccccg agcggaacat atttaacgcg
    96361 ccaatacggc ggacgaagcg gctattaaag atggtcatca cgaacaggaa aacaatgttt
    96421 agtgcaaagt aataaccaaa gttttccggc gcgacgtggt taatttcaat gtagacaaac
    96481 ggtccggcgc ttaagaacga gaacatcccg gcaaagctga aaccactggc cagcatataa
    96541 ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt acgaatgtga
    96601 aaaggctggc gacgctccgg tggtaaggtt tctttgatca gaaagaagat cattgccgaa
    96661 gccagaatcg ccgccacggc gaggatccag aagatgtaat gccagctcag ccacaccagc
    96721 acccagccgc caactatcgg tgccatcagc ggtgcaatgg ttgtcaccag catgacaaac
    96781 gacatcatcc gcgagaactc ttctttaggg tagatatcac gcatcaaagc gttgataacc
    96841 acgctggccg cagccgccgc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg
    96901 gtttgtgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc gccaagaacc
    96961 accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag
    97021 cccagaatat aagtactgag ggtcatctgc gtactgcccg ccggtacgcc aaactgcgct
    97081 gaaattaccg gtagcgcggg cagatacata tcaatcgaca gcggcatcaa catggccagc
    97141 aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc
    97201 tgaaagtcat tgaaaagtta gacgacgctg gcaatttctt cttcagttaa cggacgatat
    97261 tcaccggggg ctaaatcagc atccagcgta atgccgccaa tacgttcacg atgcagctca
    97321 accacatggt tacccacagc ggcgaacatg cgtttcacct gatgataacg cccttcgctg
    97381 atggtcagac gaacctgcgt tggggtaatc acttccagca ccgccggctt agtgagatct
    97441 ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacgggt
    97501 gattccagcg tcaccagata ggtcttctcg caatgatggc gcggagaagt aatgcggtgc
    97561 gaccactgac catcatctgt cattagtacc agaccggtgg tatcgatatc cagccgccct
    97621 gcggcgtgca gtttccacgc cactggttca tctagaaaat agagcaccgt tgggtgatca
    97681 gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta acgtggaccg
    97741 tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcaggaag cagtttgaac
    97801 gctgcattac ggacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg
    97861 gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg
    97921 attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact gttgtctgcc
    97981 cagtatagcg gtctaataac atccctcaag agaaaaagat ccgtggcata ctattagcag
    98041 aataatctac ctacgcgaga ccatgatttt tacacttcgc ccatatcagc aagaagccgt
    98101 ggatgccacg ctcaatcatt ttcgtcatca taaaacccct gccgttatcg tgctgcccac
    98161 cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg ctggctcgtg gtcgcgtgct
    98221 ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaaatatc aggcgctggg
    98281 actggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtggt
    98341 atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct
    98401 gttgattgtc gatgaatgtc accgtattgg tgacgatgaa gagagccagt atcagcaaat
    98461 cctcactcac ctgacaaaag tgaatcccca cttacgcctg ctggggctga ctgccacgcc
    98521 ttttcgactg ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga
    98581 tgagaaagcc cttttccgtg actgcattta tgaactgccg ctgcgttata tgattaaaca
    98641 cggctatctg acgccgccag aacgactgga tatgccagta gtgcaatacg atttcagccg
    98701 cttgcaggca cagagtaacg gcttgttcag cgaagccgat ctcaaccgcg agctgaaaaa
    98761 acagcaacgc attacgccac atatcatcag ccagatcatg gagtttgccg caacgcgcaa
    98821 aggggtgatg attttcgccg cgacggtcga acacgcaaaa gagattgtgg gattactacc
    98881 cgccgaagat gctgcactga ttactggcga cacccccggc gctgagcgcg atgtgttaat
    98941 tgaagatttt aaagcccagc gttttcgcta tctggtcaac gtcgcagtac tgaccaccgg
    99001 atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct
    99061 ttaccaacaa attgtcgggc gcggtctgcg tctcgctccg ggcaagactg attgtttaat
    99121 ccttgattat gcgggtaatc ctcacgatct ctacgcgccg gaagttggta caccgaaagg
    99181 caaaagtgac aacgtcccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt
    99241 ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgtc gctgtcaggg
    99301 atggtttgaa gatgacgacg gtcatcgcga acagtgtgac ttccgtttcc gttttaaaaa
    99361 ttgcccgcaa tgtaacgcgg aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac
    99421 cgtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag acgcgctggt
    99481 attacgctgt agcggcatgt ctttacagca tgggcacgac gagaaaggcg aatggttgaa
    99541 aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc
    99601 cgcccagcga actgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat
    99661 ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagccttac tgcgacatcc
    99721 ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga
    99781 ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat
    99841 gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta
    99901 cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca
    99961 acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca
   100021 agttcccggc aatcatctac ggtggcaaag aagcaccgct ggctatcgag ctggatcacg
   100081 acaaagtcat gaacatgcaa gctaaagctg aattctacag cgaagttctg accatcgttg
   100141 ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga
   100201 agctgcagca catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc
   100261 tccggcgggg ttttttgtat ctgcagatta tgcctgatta cggtattgct attttttgca
   100321 ggccagataa ggcgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg
   100381 ccagacgtcc ggcgctgcaa ttggtcgcgc aaattcggcg gcgtgccttt aatagtcagg
   100441 gtatcggtcg ccggatccca gaaaatccgt tcgcccaaca gcatcgcatc aaaattaata
   100501 gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc
   100561 gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg
   100621 ctgacgcctg ccagctcttt cgacagcgat tccagttcga tctcttcgcc tgcctgcagc
   100681 tgctcattac agtagctata aacctgctgg cgcacgttct gtcgctctgc tttatccagt
   100741 tgtgcttcgg cagtgaaatc atcaaccgct tgcaacagcc cacggttttg cgctttcgcg
   100801 tttaaccctt cgctggcccc gaggaaatcc ataaagaaat ccgccacttt gcgccctact
   100861 ctccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca ttcggttaaa
   100921 tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgggttgg gttgatgtcg
   100981 agattttcgt taacgcgcat actgctcagg ttgctcagaa ctgccaccag caaatactcc
   101041 accgccagat agcgatagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc
   101101 gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttca
   101161 ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa
   101221 gctttatttt tggcgctata gacccgatgc agttctgcta ccatctcaac gacggtttct
   101281 gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata
   101341 agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg
   101401 ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga
   101461 aggcggcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg
   101521 taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac
   101581 agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag
   101641 gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc
   101701 agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc
   101761 tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg
   101821 tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt
   101881 caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac
   101941 cacgcttgct ggtcgcattt attcctacgt aagcattatc ggccatttca gcttcctggt
   102001 gttcgccacc tacttgctga tcctcttccc gctgaccttt atcgtcggct cccagaggct
   102061 gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgatag
   102121 cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcaac tggttatcaa
   102181 cccagacgaa aatgagatgg cgcgcgactg gcagctgatg ttcatcagcg tgccggttat
   102241 tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg
   102301 tcgtcggcgc ttcgcgcgtc cgctggccgc attcttattt atcgccttta tcgcctcgca
   102361 tgtggtgtat atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa
   102421 cctgccgctt tcgtacccga tgacggcgcg acgttttctt gaaaagcatg gcctgcttga
   102481 tgcacaggag tatcaacgcc gtcttattga gcaaggtaat ccagacgccg tttccgttca
   102541 gtatccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg tcttgttgat
   102601 tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt
   102661 tgctgagcaa aatatttcgt tcacgcgcca tatgagctcc ggcaacacta cagacaacgg
   102721 catctttggc ctgttctatg gcatctcacc gagctatatg gacggcattc tgtcgacccg
   102781 tacgcctgcg gcgttaatta ccgctctcaa ccagcaaggc tatcagttgg gcctattctc
   102841 gtcagatggc tttaccagtc cgctttatcg tcaggcattg ttgtcagatt tctcgatgcc
   102901 gagcgtacgc acccaatccg acgagcagac cgccacgcag tggatcaact ggctgggacg
   102961 ctacgcacaa gaagataacc gctggttctc gtgggtctct ttcaatggca ccaacattga
   103021 cgacagcaat cagctggcat ttgcacggaa atatagccgg gcggcaggca atgtcgatga
   103081 ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactgggca atacggtggt
   103141 gattatcact gccggtcggg gtattccact gagcgaagag gaagaaacct ttgactggtc
   103201 ccacggtcac ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat
   103261 taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt
   103321 cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgtca
   103381 ttactgggtt accgcagcgg ataacgatac gctggcaatt accaccccga aaaagacgct
   103441 ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga
   103501 tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc gttttatcgc
   103561 taactgatta attataaatc agttagcgat atatcttact tgcaatcggt gtggaaaacg
   103621 gtagtattaa cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt
   103681 gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaaa aaaaccaacc
   103741 gtaaaggttg gtttttttat atctgcattt aattcgataa acagaccgcg acacatcacg
   103801 gcctgtttat tgtctgttat cagaacgtcc agaccacacc cgcctgggta ttccacggcg
   103861 attctacccc tgcgccattg ccatagctga ctgacaaatg accgcttaac gtcggggtaa
   103921 acgatgacct tatcccagcc tgataaacgc cactggttcc agccacatca ttattaaatt
   103981 tgccatcgtc attaactttc acctggttag aatcggcgta ttcctgacgc acggcggctt
   104041 tcagccaggg ttccagcgtc gtaccgtttt gcaggtccat gtgatagctt accgccgttc
   104101 ccgcttcagc gcgtaatatc cgggtatttc ccacgtccgc gcgcatgccg tttgataacg
   104161 tgtagtcctg accatctgtg gtaaagccgg taaaggccag atagggtcta acactccaca
   104221 atccgtcaac ccaacggaac ccgctctcaa catgagcacc cgcgccgtta ctattgtaat
   104281 cgccaaacgc tgttgcccca ttactcatct tgccatggat ggtgttggca aaacggtcaa
   104341 ctttcaccac cccatcaaca taggcaccgt tctgatgctc ccaaccggca taagcaccca
   104401 gggtatagct atcgacattg cctttgccgc cgcgatcaaa accaatatca gaatgagagt
   104461 aaccaaagaa caagccgcga attgtgctgc tttcttcacg ggagaaacgg ctatcgatac
   104521 cgagcgtcag gcccgtcaat gtttgctcaa aaccagctcc cgcatcagtg gtcacgttgt
   104581 tgcgggtgtt aattgccgaa ctccacatcg ccgtatcgta actaacgcct tttacgctac
   104641 caagacgctc acgcacggtg tccagttctg catcaaatac cagcggttgt gcggccgcca
   104701 tattcagcac atcagtggtt gaaggggtaa tttgcgcgcg attctctgcc agactccagc
   104761 tatggttgcc attatccagc aaggtatatt catacgtacc gatatcaaca acgcctccgg
   104821 cattgcccaa cgtaaatgca gcatcaccgc cgcccgttgt taccagtgta aggctatctc
   104881 ctgctgccgg gctggcaccg gtgtccgtca cgaatatttt gaaatcacct gttgcctgac
   104941 cggtgacgtt gagctgatcg ctctgatgat tagccatatc ggtacgcata taaaaattgc
   105001 cgtttcccga cagggtattg gtggtcaacg taatgaaatt tttcgcactt gccgtcactg
   105061 atgaacgagt cactggctca tccatcataa tcacatttgc gtcagacatc gcaaggttgg
   105121 ctaagttgag gagggtgttt tggttcgtcg tagtgatatc ggcaatgatg gtccatacgc
   105181 tattttcgag cgaaacgtcc gctttgctgg tatccacggc accatgcgtt ctaaaagaag
   105241 cgccctcaga aaccactacc ttacccacca acattgcggg tgcataattt atttgcggct
   105301 tcatgacctc aagaatgcca tcattccctc tgactgacac gttaaccatt gtgccagccc
   105361 agacgttagc gcggccattg ttgataacca tattttcaga gatggctttt gaggaatacg
   105421 tgatacttcc attctgatct gatcgaccga gatcatatac cgcatttgcc tgcactctcg
   105481 tcccagtatc ctttcccagc gattgcatag tggcatgctc atcaaggata gtgtcaatgg
   105541 cctgcgtgtc ctccataaca ataagcccgg aaccatcatc cagttcataa ttttttgaca
   105601 caccatcttt tatactaaat tggcctttac tgtttgtacc actcacttcc agcgcattcg
   105661 ttgagacaat taattttccg cctgcttttt tatcaacgcc agttgcagtc ccgccatcca
   105721 taacctccag taagccgcca ctatctacag tagtattata agcgaagtca ttttcttcaa
   105781 ctcgcaaact tccgccgttt tccaacagca tattgtttgc aattccattc ttgatatcga
   105841 actgaccgag acggtttgtt ccgaatacct ccatgacccg cgtggttgct ttaatagcac
   105901 cgcctgcctt ctgatctacc gcaaatgcta atcccccgtc tttaacctcc agtaatcctc
   105961 ccttttcgat agcggcaaag acggcaatcc catctttttc aacaattaag cgcccgcctt
   106021 taatcgtggg atcattcgcc gcgccataaa cctgtagcac accattattg agcgtagtct
   106081 ggtttgccgc gccacccatt tgaacaattt gataaccatc atttaatact gagtattctg
   106141 cggtgccatt catttcaatg tactgatacc cgccgttaat ttcagtatta ctacttatac
   106201 caaattcttt aatatgctgt tctccgccag gattgatttt cgtattattg gcggtcccat
   106261 aaaccagctg gatatctttt tccaggacaa caccatcaac tgtctcacca tactcaactg
   106321 ttgatgccaa tgctatgggt gaatataacg ctgtagccac cagaacagat attgcggaac
   106381 gacaaagaga aacagaacca gattgatgca ttgagctttc atcctatgaa attaattgct
   106441 gttaaaagca ttgggtagag aaaataccca tagctccata cccggagtca gtttttaaaa
   106501 actgtttaaa gaaatgcaca agtattgtga ttgatttttt agttgttttt cttgatgaga
   106561 agctgatgca aaattccgtc tttataatga aaatgatgcc aaagcgaacg acaaggttgt
   106621 agttttcgct acatgtccat acataaaatg gggtaacatt cacgcgcctg gtagcgttac
   106681 caacgctacg ctcaaacata atgattctaa taaaacctca ggagactact atgcctgaag
   106741 caacgccttt tcaggtgatg attgtggatg atcatccact tatgcgacgc ggtgttcgtc
   106801 agttactgga gcttgatcgt ggctttgaag tggtcgccga agcaggcgac ggcgcgagcg
   106861 cgatcgatct ggcgaataga ctggatatcg acgtgatctt gctggatctc aatatgaaag
   106921 gtatgagtgg cctggatacc ctcaatgcct tgcgcaggga tggcgttacc gcgcaaatta
   106981 ttatcctgac cgtatcagat gcctccagcg atgtctttgc gctgatagac gcaggcgcag
   107041 acggttatct gttgaaagac agcgacccgg aagtgttgct ggaagcgatt cgtgccggag
   107101 cgaaaggcag caaagtcttt agcgaacgcg tcaatcagta cttacatgaa cgtgaaatgt
   107161 ttggcgcgga agaagatccc ttcagcgtgc tgacggagcg cgagctggat gttctgcacg
   107221 agctggcaca ggggctgtca aataaacaga ttgcctcggt gttgaatatt tccgagcaga
   107281 cagtaaaagt acatattcgc aatctgctgc gcaaactcaa tgtccgctca cgcgtggcag
   107341 ccaccattct gttcctgcaa caacgcgggg cacaataaaa atagcccgat ggcatcacca
   107401 tcgggcctct ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact
   107461 acgttcaatc accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca
   107521 cgcggcaacg gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttaa
   107581 cacacggata ttgctgtggt cgcttctcac caactggcgc agcagttcgc cgccgaggcg
   107641 gttgtcgttg ggatcagatg aacgtgtcaa cgcttcagca taacccagtg cggcatcgct
   107701 gtttttcgga tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc
   107761 cagcgccatg ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg
   107821 cagttgagta cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc
   107881 tttcggatcc agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat
   107941 tttcacctgc tgataattgc cagtctggta gtagctgacg ccagctacaa ttaacgccac
   108001 taaaataccc ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc
   108061 aggaaacgct tccggtacca cgcgtacccg acgccgcgag cgggcgtaaa tgacccaacc
   108121 gccaatacca atagccacta ccggaagcac ccacagcagc acggtcagcg gcgttaacgg
   108181 cggatcgtaa gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttctt
   108241 acttttgcct tcctgcatca gttcatacac tttctggcgc aggtcggtgg caatcatcga
   108301 gttggaatcg gcaatgctgt tattctgaca tttcgggcag cgcagttctt cagtgagctg
   108361 acggaactgt tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc
   108421 tgagccggag atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta
   108481 ctgtatttct cccacagcgg cttgatctct tcttcccaga cgcgaggatt cagatcgccc
   108541 gcatggcgat agcgaatgat gccgttgccg tcaataagga acgtttctgg cgcgccatag
   108601 acaccgagat ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg
   108661 cccagctctt tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc
   108721 acgcggatgc cctgcgcaga aagctgattc agatattgat gttccgcacg gcaggtcgga
   108781 caccaggtcg cccagacgtt aagcagcact ggtttgccct gagtcagcac atccgcctga
   108841 taaaactgcc ccggattgtc cagtgattcg agacgaaact tcggcactgg cttgccaatg
   108901 agcgccgatt ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc
   108961 agcgccgcgg caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata
   109021 cagcctccgg cgcagttttc tgcgggttca cgcgcttacg atagcgagga tcaaacagg
//
LOCUS       DAPGRN010000003        90833 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPGRN010000003 DAPGRN010000000
VERSION     DAPGRN010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 90833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 90833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..90833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.003"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..668
                     /locus_tag="RHW33_000207"
     CDS             84..668
                     /locus_tag="RHW33_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1643761.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGV
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            748..1443
                     /locus_tag="RHW33_000208"
     CDS             748..1443
                     /locus_tag="RHW33_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1643762.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PIANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGLVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKADLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1473..3989
                     /locus_tag="RHW33_000209"
     CDS             1473..3989
                     /locus_tag="RHW33_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1643763.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GAPGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTYVLQGTAQCH"
     gene            4000..5091
                     /locus_tag="RHW33_000210"
     CDS             4000..5091
                     /locus_tag="RHW33_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1643764.1"
                     /translation="MKRAPLMTGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5134..5994)
                     /gene="rsmI"
                     /locus_tag="RHW33_000211"
     CDS             complement(5134..5994)
                     /gene="rsmI"
                     /locus_tag="RHW33_000211"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV1643765.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6059..8095
                     /gene="lpoA"
                     /locus_tag="RHW33_000212"
     CDS             6059..8095
                     /gene="lpoA"
                     /locus_tag="RHW33_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV1643766.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTKINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRLEKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQVRYWRAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8053..8448
                     /locus_tag="RHW33_000213"
     CDS             8053..8448
                     /locus_tag="RHW33_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV1643767.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8468..9058
                     /gene="diaA"
                     /locus_tag="RHW33_000214"
     CDS             8468..9058
                     /gene="diaA"
                     /locus_tag="RHW33_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV1643768.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9068..9643
                     /gene="yraP"
                     /locus_tag="RHW33_000215"
     CDS             9068..9643
                     /gene="yraP"
                     /locus_tag="RHW33_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV1643769.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9757..10797)
                     /locus_tag="RHW33_000216"
     CDS             complement(9757..10797)
                     /locus_tag="RHW33_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1643770.1"
                     /translation="MTGQSSSQAATPIQWWKPTLFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLMMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10870..11505)
                     /locus_tag="RHW33_000217"
     CDS             complement(10870..11505)
                     /locus_tag="RHW33_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV1643771.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11633..12151
                     /gene="yhbO"
                     /locus_tag="RHW33_000218"
     CDS             11633..12151
                     /gene="yhbO"
                     /locus_tag="RHW33_000218"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV1643772.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12131..12574)
                     /locus_tag="RHW33_000219"
     CDS             complement(12131..12574)
                     /locus_tag="RHW33_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV1643773.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDVQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            12625..12927
                     /gene="yhbQ"
                     /locus_tag="RHW33_000220"
     CDS             12625..12927
                     /gene="yhbQ"
                     /locus_tag="RHW33_000220"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV1643774.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKLQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12914..13417)
                     /locus_tag="RHW33_000221"
     CDS             complement(12914..13417)
                     /locus_tag="RHW33_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1643775.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13411..13935)
                     /locus_tag="RHW33_000222"
     CDS             complement(13411..13935)
                     /locus_tag="RHW33_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV1643776.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14144..15139
                     /locus_tag="RHW33_000223"
     CDS             14144..15139
                     /locus_tag="RHW33_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV1643777.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15148..16026
                     /locus_tag="RHW33_000224"
     CDS             15148..16026
                     /locus_tag="RHW33_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV1643778.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16107..17114
                     /locus_tag="RHW33_000225"
     CDS             16107..17114
                     /locus_tag="RHW33_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HDV1643779.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17231..18475)
                     /gene="mtr"
                     /locus_tag="RHW33_000226"
     CDS             complement(17231..18475)
                     /gene="mtr"
                     /locus_tag="RHW33_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV1643780.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18629..20518)
                     /gene="deaD"
                     /locus_tag="RHW33_000227"
     CDS             complement(18629..20518)
                     /gene="deaD"
                     /locus_tag="RHW33_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV1643781.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFSGERREGGRNFSAERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20511..20591)
                     /gene="yrbN"
                     /locus_tag="RHW33_000228"
     CDS             complement(20511..20591)
                     /gene="yrbN"
                     /locus_tag="RHW33_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV1643782.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20698..21582)
                     /gene="nlpI"
                     /locus_tag="RHW33_000229"
     CDS             complement(20698..21582)
                     /gene="nlpI"
                     /locus_tag="RHW33_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV1643783.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21691..23826)
                     /gene="pnp"
                     /locus_tag="RHW33_000230"
     CDS             complement(21691..23826)
                     /gene="pnp"
                     /locus_tag="RHW33_000230"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV1643784.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24073..24342)
                     /gene="rpsO"
                     /locus_tag="RHW33_000231"
     CDS             complement(24073..24342)
                     /gene="rpsO"
                     /locus_tag="RHW33_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV1643785.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24491..25435)
                     /gene="truB"
                     /locus_tag="RHW33_000232"
     CDS             complement(24491..25435)
                     /gene="truB"
                     /locus_tag="RHW33_000232"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV1643786.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25435..25836)
                     /gene="rbfA"
                     /locus_tag="RHW33_000233"
     CDS             complement(25435..25836)
                     /gene="rbfA"
                     /locus_tag="RHW33_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV1643787.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26001..28673)
                     /gene="infB"
                     /locus_tag="RHW33_000234"
     CDS             complement(26001..28673)
                     /gene="infB"
                     /locus_tag="RHW33_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV1643788.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28698..30185)
                     /gene="nusA"
                     /locus_tag="RHW33_000235"
     CDS             complement(28698..30185)
                     /gene="nusA"
                     /locus_tag="RHW33_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV1643789.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLTFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30213..30665)
                     /gene="rimP"
                     /locus_tag="RHW33_000236"
     CDS             complement(30213..30665)
                     /gene="rimP"
                     /locus_tag="RHW33_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV1643790.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30872..30948)
                     /locus_tag="RHW33_000237"
     tRNA            complement(30872..30948)
                     /locus_tag="RHW33_000237"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30912..30914),aa:Met,seq:cat)
     gene            31296..32639
                     /gene="argG"
                     /locus_tag="RHW33_000238"
     CDS             31296..32639
                     /gene="argG"
                     /locus_tag="RHW33_000238"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV1643791.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32647..34272)
                     /locus_tag="RHW33_000239"
     CDS             complement(32647..34272)
                     /locus_tag="RHW33_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV1643792.1"
                     /translation="MTVFNKFARSFKSHWLLYLSVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYSPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKNAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVMSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34731..34817)
                     /locus_tag="RHW33_000240"
     tRNA            complement(34731..34817)
                     /locus_tag="RHW33_000240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34781..34783),aa:Leu,seq:gag)
     gene            complement(34832..35164)
                     /gene="secG"
                     /locus_tag="RHW33_000241"
     CDS             complement(34832..35164)
                     /gene="secG"
                     /locus_tag="RHW33_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV1643793.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35392..36729)
                     /gene="glmM"
                     /locus_tag="RHW33_000242"
     CDS             complement(35392..36729)
                     /gene="glmM"
                     /locus_tag="RHW33_000242"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV1643794.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36722..37570)
                     /gene="folP"
                     /locus_tag="RHW33_000243"
     CDS             complement(36722..37570)
                     /gene="folP"
                     /locus_tag="RHW33_000243"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV1643795.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37660..39594)
                     /gene="ftsH"
                     /locus_tag="RHW33_000244"
     CDS             complement(37660..39594)
                     /gene="ftsH"
                     /locus_tag="RHW33_000244"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV1643796.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39694..40323)
                     /gene="rlmE"
                     /locus_tag="RHW33_000245"
     CDS             complement(39694..40323)
                     /gene="rlmE"
                     /locus_tag="RHW33_000245"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV1643797.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40449..40742
                     /gene="yhbY"
                     /locus_tag="RHW33_000246"
     CDS             40449..40742
                     /gene="yhbY"
                     /locus_tag="RHW33_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV1643798.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40898..41374)
                     /gene="greA"
                     /locus_tag="RHW33_000247"
     CDS             complement(40898..41374)
                     /gene="greA"
                     /locus_tag="RHW33_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV1643799.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41622..43055
                     /gene="dacB"
                     /locus_tag="RHW33_000248"
     CDS             41622..43055
                     /gene="dacB"
                     /locus_tag="RHW33_000248"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV1643800.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43096..44268)
                     /gene="cgtA"
                     /locus_tag="RHW33_000249"
     CDS             complement(43096..44268)
                     /gene="cgtA"
                     /locus_tag="RHW33_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV1643801.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44284..45249)
                     /locus_tag="RHW33_000250"
     CDS             complement(44284..45249)
                     /locus_tag="RHW33_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1643802.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45376..45633)
                     /gene="rpmA"
                     /locus_tag="RHW33_000251"
     CDS             complement(45376..45633)
                     /gene="rpmA"
                     /locus_tag="RHW33_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV1643803.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45654..45965)
                     /gene="rplU"
                     /locus_tag="RHW33_000252"
     CDS             complement(45654..45965)
                     /gene="rplU"
                     /locus_tag="RHW33_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV1643804.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46224..47195
                     /gene="ispB"
                     /locus_tag="RHW33_000253"
     CDS             46224..47195
                     /gene="ispB"
                     /locus_tag="RHW33_000253"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV1643805.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47424..47702
                     /gene="sfsB"
                     /locus_tag="RHW33_000254"
     CDS             47424..47702
                     /gene="sfsB"
                     /locus_tag="RHW33_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV1643806.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47750..49009)
                     /gene="murA"
                     /locus_tag="RHW33_000255"
     CDS             complement(47750..49009)
                     /gene="murA"
                     /locus_tag="RHW33_000255"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV1643807.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49064..49318)
                     /gene="ibaG"
                     /locus_tag="RHW33_000256"
     CDS             complement(49064..49318)
                     /gene="ibaG"
                     /locus_tag="RHW33_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV1643808.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49478..49771)
                     /gene="mlaB"
                     /locus_tag="RHW33_000257"
     CDS             complement(49478..49771)
                     /gene="mlaB"
                     /locus_tag="RHW33_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV1643809.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGVTCIDL
                     SRVSRVDTGGLALLLHLIELAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49771..50406)
                     /gene="mlaC"
                     /locus_tag="RHW33_000258"
     CDS             complement(49771..50406)
                     /gene="mlaC"
                     /locus_tag="RHW33_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV1643810.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50425..50976)
                     /gene="mlaD"
                     /locus_tag="RHW33_000259"
     CDS             complement(50425..50976)
                     /gene="mlaD"
                     /locus_tag="RHW33_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV1643811.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50981..51763)
                     /gene="mlaE"
                     /locus_tag="RHW33_000260"
     CDS             complement(50981..51763)
                     /gene="mlaE"
                     /locus_tag="RHW33_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV1643812.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51771..52580)
                     /gene="mlaF"
                     /locus_tag="RHW33_000261"
     CDS             complement(51771..52580)
                     /gene="mlaF"
                     /locus_tag="RHW33_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV1643813.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52790..53767
                     /locus_tag="RHW33_000262"
     CDS             52790..53767
                     /locus_tag="RHW33_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV1643814.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53781..54767
                     /gene="kdsD"
                     /locus_tag="RHW33_000263"
     CDS             53781..54767
                     /gene="kdsD"
                     /locus_tag="RHW33_000263"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV1643815.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54788..55354
                     /gene="kdsC"
                     /locus_tag="RHW33_000264"
     CDS             54788..55354
                     /gene="kdsC"
                     /locus_tag="RHW33_000264"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV1643816.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55351..55926
                     /gene="lptC"
                     /locus_tag="RHW33_000265"
     CDS             55351..55926
                     /gene="lptC"
                     /locus_tag="RHW33_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV1643817.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDSTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55895..56452
                     /gene="lptA"
                     /locus_tag="RHW33_000266"
     CDS             55895..56452
                     /gene="lptA"
                     /locus_tag="RHW33_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV1643818.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56459..57184
                     /gene="lptB"
                     /locus_tag="RHW33_000267"
     CDS             56459..57184
                     /gene="lptB"
                     /locus_tag="RHW33_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV1643819.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57232..58665
                     /gene="rpoN"
                     /locus_tag="RHW33_000268"
     CDS             57232..58665
                     /gene="rpoN"
                     /locus_tag="RHW33_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV1643820.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58688..58975
                     /gene="hpf"
                     /locus_tag="RHW33_000269"
     CDS             58688..58975
                     /gene="hpf"
                     /locus_tag="RHW33_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV1643821.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59093..59584
                     /gene="ptsN"
                     /locus_tag="RHW33_000270"
     CDS             59093..59584
                     /gene="ptsN"
                     /locus_tag="RHW33_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV1643822.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59630..60484
                     /gene="rapZ"
                     /locus_tag="RHW33_000271"
     CDS             59630..60484
                     /gene="rapZ"
                     /locus_tag="RHW33_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV1643823.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60481..60753
                     /gene="npr"
                     /locus_tag="RHW33_000272"
     CDS             60481..60753
                     /gene="npr"
                     /locus_tag="RHW33_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV1643824.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60966..61598
                     /gene="yrbL"
                     /locus_tag="RHW33_000273"
     CDS             60966..61598
                     /gene="yrbL"
                     /locus_tag="RHW33_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV1643825.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALASREA"
     gene            complement(61595..62323)
                     /gene="mtgA"
                     /locus_tag="RHW33_000274"
     CDS             complement(61595..62323)
                     /gene="mtgA"
                     /locus_tag="RHW33_000274"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV1643826.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAYNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62320..62973)
                     /gene="elbB"
                     /locus_tag="RHW33_000275"
     CDS             complement(62320..62973)
                     /gene="elbB"
                     /locus_tag="RHW33_000275"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV1643827.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSQCTVDRELKVLAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63203..65539)
                     /gene="arcB"
                     /locus_tag="RHW33_000276"
     CDS             complement(63203..65539)
                     /gene="arcB"
                     /locus_tag="RHW33_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV1643828.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65635..66564)
                     /locus_tag="RHW33_000277"
     CDS             complement(65635..66564)
                     /locus_tag="RHW33_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV1643829.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWIPPTE"
     gene            67239..71699
                     /gene="gltB"
                     /locus_tag="RHW33_000278"
     CDS             67239..71699
                     /gene="gltB"
                     /locus_tag="RHW33_000278"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV1643830.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71712..73130
                     /gene="gltD"
                     /locus_tag="RHW33_000279"
     CDS             71712..73130
                     /gene="gltD"
                     /locus_tag="RHW33_000279"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV1643831.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVQAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVSA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73314..74441
                     /locus_tag="RHW33_000280"
     CDS             73314..74441
                     /locus_tag="RHW33_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV1643832.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPAETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLEPGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGVGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRLR"
     gene            complement(74501..74965)
                     /locus_tag="RHW33_000281"
     CDS             complement(74501..74965)
                     /locus_tag="RHW33_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV1643833.1"
                     /translation="MMMGEVQSLPSAGLHPVLQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQ
                     LCSAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74962..75837)
                     /gene="nanK"
                     /locus_tag="RHW33_000282"
     CDS             complement(74962..75837)
                     /gene="nanK"
                     /locus_tag="RHW33_000282"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV1643834.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75834..76523)
                     /locus_tag="RHW33_000283"
     CDS             complement(75834..76523)
                     /locus_tag="RHW33_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV1643835.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPDIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76571..78061)
                     /gene="nanT"
                     /locus_tag="RHW33_000284"
     CDS             complement(76571..78061)
                     /gene="nanT"
                     /locus_tag="RHW33_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV1643836.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGRAKNDLVKTKS"
     gene            complement(78170..79063)
                     /gene="nanA"
                     /locus_tag="RHW33_000285"
     CDS             complement(78170..79063)
                     /gene="nanA"
                     /locus_tag="RHW33_000285"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV1643837.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79185..79976)
                     /gene="nanR"
                     /locus_tag="RHW33_000286"
     CDS             complement(79185..79976)
                     /gene="nanR"
                     /locus_tag="RHW33_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV1643838.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80355..81722
                     /gene="dcuC"
                     /locus_tag="RHW33_000287"
     CDS             80355..81722
                     /gene="dcuC"
                     /locus_tag="RHW33_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV1643839.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPTKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHKLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(81764..82261)
                     /gene="sspB"
                     /locus_tag="RHW33_000288"
     CDS             complement(81764..82261)
                     /gene="sspB"
                     /locus_tag="RHW33_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV1643840.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82267..82905)
                     /gene="sspA"
                     /locus_tag="RHW33_000289"
     CDS             complement(82267..82905)
                     /gene="sspA"
                     /locus_tag="RHW33_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV1643841.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83300..83692)
                     /gene="rpsI"
                     /locus_tag="RHW33_000290"
     CDS             complement(83300..83692)
                     /gene="rpsI"
                     /locus_tag="RHW33_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV1643842.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(83708..84136)
                     /gene="rplM"
                     /locus_tag="RHW33_000291"
     CDS             complement(83708..84136)
                     /gene="rplM"
                     /locus_tag="RHW33_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV1643843.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84355..85482)
                     /gene="zapE"
                     /locus_tag="RHW33_000292"
     CDS             complement(84355..85482)
                     /gene="zapE"
                     /locus_tag="RHW33_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV1643844.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            85676..86074
                     /locus_tag="RHW33_000293"
     CDS             85676..86074
                     /locus_tag="RHW33_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV1643845.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86228..87595
                     /gene="degQ"
                     /locus_tag="RHW33_000294"
     CDS             86228..87595
                     /gene="degQ"
                     /locus_tag="RHW33_000294"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV1643846.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            87685..88752
                     /gene="degS"
                     /locus_tag="RHW33_000295"
     CDS             87685..88752
                     /gene="degS"
                     /locus_tag="RHW33_000295"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV1643847.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(88815..89753)
                     /gene="mdh"
                     /locus_tag="RHW33_000296"
     CDS             complement(88815..89753)
                     /gene="mdh"
                     /locus_tag="RHW33_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV1643848.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90188..90658
                     /gene="argR"
                     /locus_tag="RHW33_000297"
     CDS             90188..90658
                     /gene="argR"
                     /locus_tag="RHW33_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV1643849.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22380 a        24361 c        22564 g        21528 t
ORIGIN      
        1 tctacctgta taatctttaa agatgttgaa catatattca cattaaatat gattatgtac
       61 ttgttacaag gataaggtta taaatgaata aagttacaaa aacagctatt gcgggcttac
      121 ttgcgctttt cgcaggtaat gccgctgcaa ccgatggtga aatagttttt gatggcgaga
      181 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
      241 actataatgc cgaacagttt cgttctgtag gcgatcgtag cccaaaaatt ccattcacta
      301 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      361 ctttccgttt atggctggaa acgcgggata atggtaccgt ccctaacttc cctaacttgg
      421 caaaagtggg ctcttttgct ggcacagcag caacaggtgt gggtatccgt attgacgatg
      481 cggaaagcgg aaatcttatg ccactaaatg ctatgggcaa tgataacacg gtttatcaga
      541 tccctgcgga ctccgctgga gttgttaatg ttgacctcat cgcttactac gtatcaaccg
      601 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      661 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      721 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gataataacc
      781 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      841 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctatcgcaaa caaaggcgca
      901 gaaacacctt atttactgca atcatgggta gataatatag atggtacaag ccgtgcccca
      961 tttattataa ccccaccgct atttcgtctt gaagctggcg atgattcatc actgcgaatt
     1021 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
     1081 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
     1141 aaaacacgga tcaaaatgtt ttatcgcccc gcacacttga aggggttggt caacgatgcg
     1201 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1261 tattacgtcg tatttgccgg actggcagtc gataaagccg atctcacaag caaaattgaa
     1321 tatatcgcgc ccggagaaca taaacagtta ccacttcctg catctggcgg aaagaacgtg
     1381 aaatgggctg cgatcaatga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1441 taacaaatat aaaaaacaca ggtcatcagg gaatgccaca acgacaccac cagggacata
     1501 aacgcacacc gaaacagtta gcgctcatta tcaaacgctg tttgccgatg gtgctcactg
     1561 gcagcggcat gctttgcact accgctaacg ccgaagagta ttatttcgac cccattatgc
     1621 tggaaaccac aaaaagtggt atgcaaacaa ccgatctgtc acgtttttca aaaaaatacg
     1681 cacaactacc aggaacttat caggttgata tctggctgaa taaaaagaag gtttcacaga
     1741 aaaaaattac atttaccgcc aatgcagagc aacttctgca gccacagttt acggtagaac
     1801 aactacgtga gctgggtatt aaggtggatg aaatcccggc gctggctgaa aaagatgacg
     1861 atagcgtgat caactcgctt gaacaaatca ttcccggtac agctgctgaa tttgatttca
     1921 atcatcagcg acttaatttg agcattcccc aaattgcact gtaccgtgat gcaagaggtt
     1981 acgtctcccc ttctcgttgg gacgatggta taccaacgct gtttaccaac tactcgttta
     2041 caggttctga taaccgttac cgccagggca atcgtagcca acgacagtac ctgaatatgc
     2101 aaaatggtgc taattttggc ccctggcgat tacgtaacta ttctacgtgg acacgcaacg
     2161 atcaggcgtc aagctggaac actatcagta gttatttaca acgtgatatc aaggcgttga
     2221 agtctcagtt gcttctggga gaaagcgcca ccagcggcag tattttttcc agctacacct
     2281 ttactggcgt gcaactcgct tccgacgata atatgttgcc aaacagccag cgcggatttg
     2341 ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt gactatcagg caaaatggtt
     2401 atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga aattaacgat ctctacccct
     2461 cttccaacag cggcgattta gaagtcacga ttgaagaaag tgacggtaca caacgtcgct
     2521 ttatccagcc ttattcttca ttacccatga tgcagcgacc tgggcatcta aagtatagcg
     2581 cgaccgctgg acgctatcgc gctgatgcaa acagtgatag caaggaaccc gaatttgctg
     2641 aagccacggc aatatatggt ttgaataata cttttacgct gtatggcggc ctgctcggtt
     2701 ctgaagatta ttatgcgctg gggatcggta tcggcggcac acttggcgca ctgggcgcgt
     2761 tgtcgatgga tatcaacaga gctgacaccc aattcgataa ccagcactct tttcatggct
     2821 atcaatggcg tacgcagtac atcaaagata tcccggaaac caacaccaat atcgctgtca
     2881 gctactatcg ctataccaac gatggctatt ttagttttga tgaagccaat acccgcaatt
     2941 gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa catcagccag acaatatttg
     3001 atggggtaag tctgtatgcc tccggttcac agcaagacta ttggggcaat aacgagaaaa
     3061 acaggaatat ctctgttggg gtttccggcc agcaatgggg aattggttac agcctgaatt
     3121 atcaatacag ccgctacact gatgaaaata atgaccgtgc actctctttg aatctcagta
     3181 ttccgttaga acgctggtta ccgcgtagcc gggtttccta tcagatgacc agccagaaag
     3241 atcgcccaac ccaacatgaa atgcgtcttg atggctcact gctggatgat ggccgcctga
     3301 gctatagcct ggaacaaagt ctggatgacg ataacaacca taacagtagc ctgaacgcca
     3361 gttaccgttc accttatgga accttcagtg ccggatacag ttacggtaat gacagcagcc
     3421 aatacaatta cggcgttacc ggcggcgtgg ttatccatcc tcatggcgtg acgctctcgc
     3481 aatatctggg caacgctttt gcgcttatcg atgctaacgg ggcacctggc gtgaggatac
     3541 aaaactatcc ggggattgct accgatccct ttggctatgc agtggttcct tatctcacga
     3601 cttatcagga aaaccgtctc tcggtagata ctacgcagct gcccgataac gtcgatcttg
     3661 agcaaacaac acagtttgtg gtgcccaaca gaggtgcaat ggtagcggcg cgtttcaacg
     3721 ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg caacggtaaa ccgttgccct
     3781 ttggcgctct tgccagcaac gatgagacgg ggcaacaaag tatcgtcgat gagggcggca
     3841 tactatatct ctccgggata tcgagtaaat cacaaagctg gactgtacgc tggggaaatc
     3901 aggctgatca acaatgtcag tttgctttta gtacaccgga ttcagaacca acaacctatg
     3961 tattacaagg cacggcgcag tgccattaag gataaaaaaa tgaaaagagc gcctcttatg
     4021 acaggacttt tgttgatatc cacatcctgc gcttatgcct cctcaggagg gtgtggagcc
     4081 gacagcacta gcggtgcgac aaattacagc agtgtggttg atgatgttac ggtgaaccag
     4141 acagataacg tgacaggacg ggagtttacc tctgcaacgc taagtagcac taactggcaa
     4201 tacgcctgtt cctgctctgc gggtaaggca gttaaacttg tctatatggt cagccccgta
     4261 cttaccacca ctggacatca gacaggatat tacaaactca atgacagcct ggatattaaa
     4321 accacattac aggcaaacga cattccagga ctcacaaccg accaggttgt ctctgttaac
     4381 acccgattca cacagataaa aagcagcacc gtatattctg ctgcaaccca aacgggtgtt
     4441 tgccagggtg acacgtctcg ttatggaccc gttaatattg gtgcaaatac cacctttacc
     4501 ctgtatgtca ccaagccatt tctcggctcg atgaccattc cgaaaacgga tattgccgtc
     4561 attaaaggcg cgtgggtcga tggaatggga agcccgtcta caggtgactt ccatgattta
     4621 gtcaagttat cgattcaggg aaatctcacc gccccacagt cgtgcaaaat taatcagggc
     4681 gatgttatta aggttaattt tggattcatc aatggtcaga agtttaccac ccgcaatgcc
     4741 atgccagacg gttttactcc agtagacttt gatatcactt atgactgtgg tgatacttca
     4801 aagattaaaa actcgttgca aatgcgcatc gacggtacaa ctggggtagt agaccagtac
     4861 aacctggtcg ccagacgaag aagttcagac aatgcgcccg atgtcggtat tcgtattgaa
     4921 aatctcggcg gcggagttgc aaatattcct tttcagaacg gtatccttcc cgttgatcct
     4981 tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta atctggtcgg tggtgagctg
     5041 gaaacaggaa aatttcaggg cacagccacc attaccgtca tcgtgcggta aaagaagacg
     5101 ttgcgttttg attaacgcaa cgcttcggca ctcttacccc tgctgctcca gcgcatactt
     5161 atatagcgca tttttcttca cgccgtgaat ttctgctgcc agcgccgccg cttttttcag
     5221 cggcagttct gcctgcaaca gcgccagcgt gcgcagggca tcggcgggta agtcttcttc
     5281 ctgtgcttta tgaccttcga caatcagcac catttcgcct ttgcgacggt tttcatcttc
     5341 ctttacccac gccagcagct cgccaacggg cgcgccgtga atggtttccc aggttttggt
     5401 cagctcacgt gccagaacca cgtagcggga ttcgcctaat accgcaacga tatcttccag
     5461 gctatctaac agacggtggg tagattcata aaaaatcagc gtgcgcggct ccgcttcaat
     5521 ggcttttagc gcatcacggc ggccttttga tttggcaggt aaaaagcctt cgtaacagaa
     5581 acggtcagag ggtaaacccg cggcgcttaa cgcagtgata gcagcacacg gtccgggtag
     5641 cggcaccaca cgaatccccg cttcacggca ggtacgcacc aaatggtagc caggatcgtt
     5701 aattagcggc gttccggcat cggaaaccag cgcaatgttt tgcccctctt gcagcttcgc
     5761 cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc agcgcaaaca accgggcatt
     5821 aatcccaaaa tgttgcagca ataaaccggt gtgacgagta tcctcggcgg caatcagatc
     5881 aacggcctgt aatacctcta acgcacgctg ggtgatatcc gccagattgc cgattggcgt
     5941 cggtacaatg taaagctggc cctgagaatt atccgccgat tggtgttgtt tcattgtgtc
     6001 gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa aatatcactg gatacattat
     6061 ggtaccctca acattttctc gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc
     6121 cctgattttc gccggttgtg gcacccatac tcccgatcag tccactgctt atatgcaggg
     6181 cacggcgcag gctgattctg ccttttatct tcagcagatg cagcaaagct ctgatgatac
     6241 caagatcaac tggcaattac tcgccattcg tgcactggtg aaagaaggta aaaccgggca
     6301 ggcggttgag ttgtttaacc aactaccgca agaactgaat gattctcagc gtctcgagaa
     6361 aacactgctg gcggtagaga ttaaactggc gcagaaagat tttgctggcg cgcaaaactt
     6421 gctggcgaaa attacacccg ccgatttaga acaaaaccag caagtccgtt actggcgggc
     6481 aaaaatcgat gccagccagg ggcgtccttc cattgattta ctgcgcgcgt taattgctca
     6541 ggaaccgctg cttggcgcga aagaaaaaca gcagaatatt gatgccacct ggcaggcgct
     6601 ctcctccatg actcaggaac aggcgaatac gctggtgatc aacgccgacg aaaatattct
     6661 gcaaggctgg ctggatctgc agcgcgtctg gtttgataac cgtaacgatc ccgacatgat
     6721 gaaagccggg atcgccgact ggcagaaacg ttatccgaac aacccgggcg cgaaaatgct
     6781 gccaacgcag ttggttaacg taaaagcgtt taaaccagcc tcgaccaaca aaatcgccct
     6841 gctgttgcca ctgaatggcc aggcagcggt atttggtcgc actattcagc aaggctttga
     6901 agcggcgaaa aatatcggca ctcagccagt ggcagctcag gtagctgccg cacctgccgc
     6961 agacgttgca gaacaacctc aaccgcaaac cgtggatggc gttgccagcc cggcacaagc
     7021 ctcggttagc gatctgaccg gtgaacagcc tgcagcccag ccggtgcctg taagcgcccc
     7081 ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc tgaaaatcta
     7141 cgatacctca tcacaaccac ttagtcagat cttaagccag gttcagcagg atggcgcgag
     7201 tattgtggtc ggtccgttgc tgaaaaataa cgttgaagag ttgctgaaga gcaacactcc
     7261 gctgaacgta ctggcactga accagccgga gaatatcgaa aatcgcgtca atatttgtta
     7321 cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg
     7381 taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg tagccaatgc
     7441 gtttgcgcaa gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa aatttggttc
     7501 caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa cgggtacccc
     7561 gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa cgctgcaaac
     7621 cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt
     7681 ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta acggtagtca
     7741 gagcggtgca acgctgtacg ccagctcccg cagtgcgcaa gggaccgctg gcccggattt
     7801 ccgactggag atggaaggct tgcagtacag cgaaatcccg atgctggcag gcggtaatct
     7861 accgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg ctcgcatgta
     7921 tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc gccaggttca
     7981 gggttttgaa atcaacggta ataccggaag cctgacggct aacccggatt gcgtgattaa
     8041 caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca gttaaccacc
     8101 aaacagaccg gcgatgcgtg ggaagtacaa gcgcgccgct ggctggaagg caaaggactg
     8161 cgatttgtcg ccgctaacgt gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa
     8221 ggccggacca ccgtttttgt cgaggtgcgt taccgccgtt ctgcgcttta tggcggcgcg
     8281 gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg cttgtggctc
     8341 gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt agccttcacc
     8401 gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt aaggtttaag
     8461 gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc
     8521 ggcggcagag gcgcttccgg atgccatctc ccgtgcggcc atgacgctgg ttcagtctct
     8581 gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca atgcacagca
     8641 ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac ctgccattgc
     8701 actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac atgatgaagt
     8761 gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag ccatttccac
     8821 ccgtggcaac agccgcgata ttgtaaaagc ggtagaagct gccgttacgc gtgatatgac
     8881 cattgtggca ttgaccggct atgacggcgg tgaacttgca ggtttgttag ggccacagga
     8941 tgtggagatc cgcatccctt cgcatcgcag tgctcgcatt caggaaatgc atatgctgac
     9001 ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg atgattaagg
     9061 agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg
     9121 ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg caactgaccc
     9181 acgcagtgtc ggcacccagg tggacgatgg taccctggaa gtgcgcgtga acagcgcatt
     9241 gtcgaaagac gaacagatta agaaagaagc gcgcattaat gtaacggcct atcagggcaa
     9301 agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca aacagattgc
     9361 tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc agccgattgg
     9421 tctgggcgaa gcgtctaacg atacgtggat caccaccaaa gtgcgttcgc agctcttaac
     9481 cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct
     9541 gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca gccgggtgag
     9601 cggcgtgaag cgggtaacta cggcgtttac gtttattaaa tagcagcccc ttgtaatgcc
     9661 tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta ggtcggataa
     9721 ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aataacagcg ccagcccgcc
     9781 gacaatcacg cctgacactg ccaccatcgc ccccgtcagc cataaggctt tcgccgggaa
     9841 cgctttgcgc agcataatca gtgacggcaa actcaccgcc gggagcgtca tcaacaatgc
     9901 cagcgccgga gcagttccca tacctgccag catcatcgtt tgtacaatcg gaatttctgc
     9961 tgccgtggga atgacaaaca agcatcctgc caccgccatc gccaccaccc acatcaggct
    10021 gttatcgaca gcaccatcgg catgggggaa taaccagacg cgagcggcac ccaacaccag
    10081 tactgcaagg atgtaaaccg ggatcgtact ccagaaaagc gtccatagcg ccctgcccca
    10141 gcggctaaaa aatccgccct gtgcttccgg tatgtcaatt tcgaccggtg cttgcgtttg
    10201 cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc agcaacacca tcatcagccc
    10261 cgccaccaga cgaatcgccg caaaacccca gccgaggacg aaccccataa acaccagcgt
    10321 cgccgggttt aacaacggat tgcccatcca gaatgccagc gcaccgccca tcgacacctg
    10381 ttggcgacgc attcccgccg cgaccggagc cgcacagcag gtacacatca tgcccggcag
    10441 cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa
    10501 ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg atcaccccca gcacagcagc
    10561 tttccatact gcgaggaaat agatcatcgc gtaatccaac gccgcttgcc acggattagc
    10621 atccgcctgc gcaaggatag atttaccgat actgtgggtt tcggcagcag taaacgcttt
    10681 gccgtagtaa ggttgccatt tcacatacca gagaccggca atgacaacga gaaagaaaag
    10741 cgtgggtttc caccactgaa tgggcgttgc cgcctgagat gaagactgac cagtcatagc
    10801 attccccagg aataagttat gataattgag tgcgtgaata ttacgctcac tatcaattct
    10861 tgggaataat tattcagctc ttttgcgtaa ttctgaagag cttaaaatcg tcacaccttc
    10921 atgctcgggc cgcatcgcct ctgccagcat gacccgcgcc acgtctctcg catcaatgga
    10981 tttccagtta ccgggtaaca aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg
    11041 tttgctacga tcgcccagta acatcgacgg gcgagcaatg gtcaatttcg gccagttctg
    11101 ggcgattaat gcttcttcca tctccccttt gacgcggtta tagaaaaacg gcgagtgggc
    11161 attggcaccc atagcactga ccaccagcat atgctgcgcg cccagtcgcc gcccggttaa
    11221 tgcggtatcc actaccagcg tgtaatcggc atgaataaac gcctctttgc tccccgcttc
    11281 tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg ggatcggtga cctgcgccag
    11341 cgcgtcagtc agttgcggat catggggatt aaacaccccg ggcatatcgc ccaacggacg
    11401 tcgcgtcggc gcggctatgg cgttaacttt cggttcgtta atcaacatcc gcagcaggtg
    11461 accgcccacc agccccgttg cgcctgtaat cagtacctga ctcatcttcg ctcctttaca
    11521 gaattgtctg ccttgcgctc cacggctcat gcaccaggct taatagacca tgaggtaatt
    11581 atccccgatt gtgagaaatt cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa
    11641 gaaaattgcc gttttaatca ctgatgaatt tgaagattca gaatttactt cacccgcaga
    11701 cgagttccgt aaagccggac acgaagtgat taccattgaa aaacaagcgg gtaaaacggt
    11761 gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa tccatcgatg aagtgacgcc
    11821 agcggagttt gatgccctgt tgctaccggg cggtcattca ccggattatc tgcgtggcga
    11881 caaccgtttt gtcaccttta cccgtgattt tgtgaatagc ggcaaacctg ttttcgccat
    11941 ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt cgggggcgca aactgaccgc
    12001 ggttaaaccg atcattattg atgttaaaaa cgcgggcgcg gaattttacg atcaggaagt
    12061 ggtggtcgat aaagatcagc tggttaccag ccgaacgccg gacgatctgc cagcgtttaa
    12121 ccgcgaggcg ttacgcctgc tcggtgcctg agttacgtag ccaaatcatt tttttaccaa
    12181 agcccagcgt gttgtcggtg aatttgattt catcgagccg gatttcccac accggggccg
    12241 atagcattct ggcaaccgga aagcgacgat tgtacgcctt gcgcgcgagg tcgctttcct
    12301 caccttccag cctgcggatc tcacctttaa actgcacgcc gcgaattaac gctaccgttt
    12361 tcggctgacc gtttaccgtt ccggcaaccg cagcctgcgg cccactcatc tgcgcgtggc
    12421 gcgttttttc ttccgtcaaa atgtagaagg cgactttctg cacatcaaac agataaaagg
    12481 cattggcgca ccagagttcc ccttcctgct gcacacacca ggtgacaaca tgttgttttg
    12541 ccagccaacg gctgatggcg gtgagtgttt ccattgctgt tctcttttat actgtgggcc
    12601 tgaccttaac atgcgacgca gacgatgaca ccctggtttc tttacttgat ccgtaccgcc
    12661 gacaataagc tttataccgg gatcaccacg gatgtcgaac gccgctatca gcagcaccaa
    12721 agcggcaaag gggcgaaagc actgcgcggg aaaggagaac tgacgctggc gttttctgcg
    12781 ccagtgggcg atcgttcgct ggcgttacgg gcggaatatc gcgttaagca actgaccaaa
    12841 ctgcaaaaag agcgtctggt agcggaaggc gcagggtttg cagagctgtt aagcagtctg
    12901 caaaccccgg agattaaaag cgattgaaat gctcgtgata ctcaaccaga ccggttacgc
    12961 cattcagcgc gtcatccgct aaacgatgta cctggaaggc actttcagtg cccggccagc
    13021 ggcaacgcag atcgtgatgc gccgccagtt caaagccgaa acgactgtac agcgccggat
    13081 cgcccagcgt caccactgcg gcatagccga actcattaag cgaatcgagt ccttcataga
    13141 ccagttggcg tgccagcccc tgtccacggt atttttcatc taccgccagc ggtgccatgc
    13201 cgacccattg caggtcttcg ccctgcacat caaccggact aaatgccaca tagccaatga
    13261 cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag aaagccatct tcacgcagat
    13321 cgtgaaccag cttcgcttcc gcatcacttt cgaatgaacg acgcagcagg gcatcaatac
    13381 ccggcgcatc aatgggaatt tctactcgaa ttagcatggt tcacctaccg atgtctgttt
    13441 ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc gccagttgca gcagcatcat
    13501 gcgtaaggct ttcggcattt gctccagttc aatggcgtcc atcaggtttt tcacatacag
    13561 ccccagctcc gtatcgcctt caatcaccag ccgacgctgg aagaagagcg tatccggatc
    13621 ctgtttacgc gccgcaatca tcagcagatc gctggcgtcg gcactaaaac tcacatcagc
    13681 ttgcgcgttc tggctgacca ccagtttgcc attcaccacc gaggtaaacc attgcaggtc
    13741 aatatcacgc acatgaatac ttaaccagcg gccttcaaga aactccagct cgccatcatc
    13801 cagcgcctga cggaattgcc agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa
    13861 tggcgtcagt tttaccggta cactcaacag agatggcccc aaatgcacaa tacgggaacg
    13921 cagtttatcc aacacgagtt ttactccctg tttcaacaat catcctattt tgccatatca
    13981 gaaaaataac atagcggtat aaatcaacaa ttccatatga aattgctgct accaccaata
    14041 caactttaac tgccttaaat caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg
    14101 ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa attatggagc tgctctgccc
    14161 tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat
    14221 cgggctaaaa gatgatacca atgcccgtca cttcgccggc cttaacttta ccgagaaaaa
    14281 attgcaggaa gcggtgagtt ttgtccatca acatcgccgc aaacttcaca tcgcgattaa
    14341 cacttttgcg catccggacg gttacgcccg ttggcagcgc gccgtagata tggcggcgca
    14401 gctgggtgcc gacgcgctga tcctcgccga cctcgccatg ctggaatacg ccgccgagcg
    14461 ttatccgcat attgagcgtc atgtgtcagt gcaggcttcg gcgaccaatg aagaggcgat
    14521 taacttttat catcgccatt ttgacgttgc ccgcgtggtg ctgccgcgcg tgttgtcgat
    14581 tcatcaggtg aaacaactgg cacgggtcac acctgtcccg ctggaagtgt ttgctttcgg
    14641 cagcctgtgc attatgtcgg aaggtcgttg ctatctgtcg tcgtatctga cgggtgagtc
    14701 gcccaacacc gtgggcgcgt gttctccggc ccgtttcgtg cgctggcagc aaacgccgca
    14761 ggggctggaa tcccgcctga acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc
    14821 aggttatccg acgctatgta aagggcgtta tctggtggac ggcgagcgct atcacgcgct
    14881 ggaagaacca accagtctca ataccctgga actgctgccg gagttaatgg cggcgaatat
    14941 tgcttcggtg aaaattgaag gccgccagcg tagcccggcg tatgtcagcc aggtggcgaa
    15001 agtctggcgt caggctatcg accgttgtaa ggccgatccg caaaacttcg taccgcaaag
    15061 cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg caaaccaccc ttggcgcgta
    15121 tcaccgtaaa tggcagtgag aagagcaatg aaatattcct tagggccagt gctgtggtac
    15181 tggccaaaag agacgctgga agaattttat cagcaggccg ccaccagcag cgccgacgtg
    15241 atttatcttg gtgaagcggt atgcagcaag cgtcgggcaa ccaaagttgg cgactggctg
    15301 gagatggcaa aatcgctcgc cgggagtggt aagcagattg tgctctccac gctggcgctg
    15361 gtgcaggcat catctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg
    15421 attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc
    15481 gccgggcacg cgctgaactg ctacaacgcg gtgacactga aaatattgct caaacagggc
    15541 atgatgcgct ggtgtatgcc agtggagctt tcccgcgact ggctggtgaa tctgcttaat
    15601 cagtgcgatg agctgggcat tcgtaaccag tttgaagtgg aagtcctgag ctacggtcat
    15661 ctgccgctgg cctactccgc ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat
    15721 gagtgtgaaa cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac
    15781 caacaagtgt ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt
    15841 aacgagctgg catccatgca gggcctggtt gatgtggtcc gcctgtcacc gcagggtact
    15901 gacactttcg cgatgctcga cgccttccgc gctaatgaaa atggcgctgc accactgccg
    15961 ttgacggcga acagcgactg taacggctac tggcggcggc tggcaggact ggagctgcaa
    16021 gcctaagtaa atagctcact ttgttaacaa ctttaactac tctttaatgc agtattaaag
    16081 attaatcggt aacaaagtga gctgttatga ctgataaaac cattgcgttt tcgctactcg
    16141 atctggcccc cattcccgaa ggttcttcag cgcgagaagc attctcccac tctctcgatc
    16201 tcgcccgtct ggctgaaaag cgcggctatc atcgctactg gctggcagaa caccacaata
    16261 tgactggcat tgccagtgct gccacgtcgg tattgattgg ctatctggcg gcgaatacca
    16321 ccacgctgca tctggggtct ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg
    16381 cagaacagtt cggcacgctc aatacactct atccggggcg aatcgatttg gggctgggtc
    16441 gtgctccggg tagtgaccag cgaaccatga tggcgctacg tcgtcatatg agcggcgata
    16501 ttgataattt cccccgcgat gtcgcggagc tggtggactg gtttgacgcc cgcgatccca
    16561 atccgcatgt gcgcccggta ccaggctatg gcgagaaaat ccccgtgtgg ttgttaggct
    16621 ccagccttta cagcgcgcaa ctggcggcgc agcttggtct gccgtttgcg tttgcctcac
    16681 acttcgcgcc ggatatgttg ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt
    16741 cggcacgact ggaaaaacca tacgcgatgg tgtgcatcaa tattatcgct gccgacagca
    16801 accgcgacgc tgaatttctg tttacctcaa tgcagcaagc ctttgtgaag ctgcgccgtg
    16861 gcgaaaccgg gcaactgccg ccgccgattc aaaatatgga tcagttctgg tcaccgtctg
    16921 agcagtatgg cgtgcaacag gcgctgagta tgtcgctggt gggcgataaa gcgaaagtgc
    16981 gtcatggttt gcagtcgatc ctgcgcgaaa ccgacgccga tgagattatg gttaacgggc
    17041 agattttcga ccaccaggcg cgcctgcatt cgtttgagct ggcgatggat gttaaggaag
    17101 agttgttggg atagtgtgtc ttaacgcggg aagccttatc cgagctggca acgctgtcct
    17161 acgtaggcct gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa
    17221 ggcccgctga ttactgatac accggcagca aattaaagct cgataagata tggaccagtg
    17281 cgttgccaac gccaaatacc agaatcagcg taatcatcgg cttgccgccc cagacgcgga
    17341 atttcgggct gccaaagcgt ttacgcgatg cgcgggccag cagtgccgga acaattgccg
    17401 cccagatggt agccgctaaa ccagcataac caatggcgta caggaatccg ttcgggaaca
    17461 acagcccccc cacaactggc ggggcaaagg tcagcaatgc cgttttcaag cggcccacag
    17521 ccgagtcgtc gaaaccaaac agatctgcca gatagtcaaa caaacccagc gttacgccga
    17581 ggaacgaact cgctaccgca aagtttgaga acacgaccag cagcaggtcc aggctacggc
    17641 tgttcagcac gccgcttaat gcttgtacca gcacatcaat gttaccgccc ttctcggcga
    17701 tgccgataaa ctccggacga gggatgttac ccatcgtcgc cagcaaccag atggtataca
    17761 gcgccagcgc catcagcgta ccgtacacca ggcatttcac gatggttttc ggatctttgc
    17821 cgtaatactt catcaggctt ggcacgttac cgtgataacc aaacgatgcc agacagaacg
    17881 gcagggtcat caacagatac ggtgcataag acgcattgct ttcggcgacg ttgaacaatg
    17941 tcgcaggctg cacatgtccc agcaggctac cgaaggtgag gaagaaggta atgactttcg
    18001 cccccagcac aatcgccgtc atgcggctca cggctttagt gctcaaccac accacaaacg
    18061 ctaccaacaa tgcaaaacca aaacccgccg cccgtgccgg gacgtttagt gacatctctg
    18121 cgaaggtgtg atgcagaatc gaaccactgg cagaaatata ggcataggtc aggatataga
    18181 gcacaaaggc aatggaaatg ccgttgacca cgttccagcc tttgcccagc aaatctttgg
    18241 tgatggtgtc aaaactcgaa ccgattctgt aattcaggtt agcttccaga atcatcaagc
    18301 cggaatgcag catacagaac caagtaaaga tcagcgccgc cattgaccag aaaaaccacg
    18361 ccccggacat gaccactggc agagaaaaca tccctgcgcc aataatggtg ccgccgataa
    18421 tcaccacgcc gccaagcagc gacggtgacg tttgggtggt ggttagtgtt gccatgaggg
    18481 cttctctcca gtgaaaaata gtgcgactgc gttgttatgt attgcactgt accagtacac
    18541 gagtacaaaa aacagaaaaa aagccccgat ggttcaaatc ggggctgtat atattatttt
    18601 acagactgtg ttcgctgttc agcgatgatt acgcatcacc accaaaacga cgacgaccgg
    18661 tagagtcatc acgacgcgga gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat
    18721 caccacgacc accttcacgg cgttcagcgc taaagttacg accgccttca cgacgctcgc
    18781 cactgaaacc acgaccaccg ccacgacgct caccgccagt atgcggctgt gcatcgccca
    18841 gcaactgcat attcatcggt ttgttgagga tgcgagtgcg cgtaaagtgt tgcagcactt
    18901 cacccggcat acctttcggc agttcgatgg tggagtgaga agcaaacagc ttgatgttac
    18961 cgatgtaacg gctgctgatg tcgccttcgt tagcaatcgc gccaacgata tgacgaactt
    19021 caacaccatc atcgcggccc acttcaatgc ggtacagctg catgtcacca acatcacgac
    19081 gttcacgacg cggacgatct tcacggtcgc cacgcgggcc acggtcgtta cgatcgcgcg
    19141 gaccacggtc atcacggtca cggaattcac gtttcggacg catcggcgca tctggcggaa
    19201 caatcagagt acgttcaccc tgtgccattt tcagcagtgc ggcggccaga gtttcgagat
    19261 ccagctcttc accttctgca gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca
    19321 gatcgctgct ttccagctgc tgctgtactt tagcggcgaa tttttccaga cggcgtttgc
    19381 ccagcagttc tgcgttcggc agttctactt ccggaatagt cagcttcata gtacgttcaa
    19441 tgttgcgcag cagacgacgc tcgcggttct caacgaacaa cagcgcgcgg ccagcacgac
    19501 ccgcacgacc ggtacgaccg atacggtgaa cgtaagactc agaatccatc gggatatcgt
    19561 agttaaccac caggctgata cgctcaacgt ccaggccacg ggctgcaacg tcggtcgcaa
    19621 tcaggatgtc cagacgacca tctttcaggc gttccagtgt ctgctcacgc agcgcctggt
    19681 tcatgtcacc gttcagcgcg gcgctgttgt agccgttacg ctcaagagct tcagccactt
    19741 ccagagtcgc atttttggta cgaacgaaga taatcgccgc atcaaaatct tccgcttcca
    19801 ggaaacgtac cagtgcttcg tttttacgca taccccagac agtccagtag ctctggctga
    19861 tgtcaggacg ggtagtcacg ctggactgaa tgcgcacttc ctgcggctct ttcataaagc
    19921 ggcgggtaat gcgacgaatc gcttccggca tggttgcaga gaacagagcg gtctgatgac
    19981 cttccgggat ctgcgccata atggtttcaa cgtcttcgat gaagcccatg cgcagcattt
    20041 cgtcagcttc atccagaacc agaccgctca gtttagagag atccagagtg ccacgtttca
    20101 ggtggtccag cagacggccc ggagtaccga caacgatctg cggcccctga cgcagggcgc
    20161 gtaattgcac gtcataacgc tggccgccgt acagggcaac cacatttacg ccgcgcatgt
    20221 gtttagagaa atccgtcatt gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca
    20281 gcaccagaat ctgtggtgct ttcagctcag gatcaagatt ctgcagcaga ggtaaagaga
    20341 atgctgcagt ttttccgctc cccgtctggg ccatacccag tacgtcgcgg ccattcagca
    20401 gatgtggaat acactccgcc tgaattggag atggtttttc gtaacccaga tcgttaaggg
    20461 cttcaaggat aggagccttc aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt
    20521 agtacgtgtg cctcaaaatt aatggcggcc agtctacata actcatcatg aaattgatca
    20581 gcaattttca ttgaaaagtg tgaaccggct caaagtaggt gtattaacga acaacaacgc
    20641 cctcacccgt taaggtgatg gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta
    20701 ttgctggtcc gattctgcca ggtcatcttg gtcctggccc aggagcgata attccaacaa
    20761 tgcgtatcgg tgctcaacaa agttatgaac gttgttggca accgccagtt tgaacagtgc
    20821 cgtggcgctg tccaaatccc ccagacttag gtagtactta cctaaataga agttggtttc
    20881 actgagatgc tcagcgagcg aggtgttatc cgttgcgtcc gccttgagcc tttccattaa
    20941 cgtttgttcg ctaatgttgc ccaggtagaa ctcgacaatg ttccatcccc actgttcctt
    21001 atccgatttt tcgaagtgct gtttcaacac ttctttagcc tgcttctcat cgagcttctg
    21061 ctcggcgaga taaagccaca gactacggaa aggatcattg ggatcgtctt gataaaacgc
    21121 cagcagatca tcttgcgcta acttgtcacg accgccgtaa tataatgcga tcccgcgatt
    21181 caagtgcgcg tagttgtaag ttggatcaag ctcaagtaca gaatcaaacg cttcataggc
    21241 agcatcaaaa ttgcctgcct gcgttaaata tatgcctaag taattgaata cttcaggcat
    21301 atccggtcgg attgccagcg cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag
    21361 actatcatac aacactccgc gctcatataa aagctgtgcg cgttcgtcat cggttaaagc
    21421 ccgactggca aggatttgtt ccatacgtgc cagaatcact tcctgctgta aagtcggttg
    21481 caatggtacc gcgaggactt cacttttacg ccaggaagta ttactgcatc ctgcaagcgt
    21541 aagtgctgtc gcaacgaaac accagcgcaa aaaaggcttc atttcccact cccgaagacc
    21601 acggttgaat gaacgtcctg ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc
    21661 ccccgccgca gcggagggca aatggcaacc ttactcgccc tgttcagcag ccggagcttc
    21721 cggtgctgca gcaggttgag actgctcagt cgcttcttta atgctcagac ggatacggcc
    21781 ctggcgatca acttccagaa ctttcaccgg tacttcctga cccatctgca ggtaatcggt
    21841 cactttctca acgcgtttgt cagcgatttg agagatgtgg accagacctt ctttaccgcc
    21901 gccgatggca acaaatgcgc caaagtcaac gatacgggtc actttaccat tgtagacgcg
    21961 gcccacttcg atttctgcag tgatctcttc gatacgacga atagcatgtt tcgctttctc
    22021 gccgtcggtc gctgcgatct tcacagtacc gtcatcttcg atttcgatgg tagtgccagt
    22081 ttcttcggtc agggcacgga taacagagcc gcctttaccg ataacgtctt tgatcttgtc
    22141 cgggttgatc ttgatggtat ggatacgcgg tgcgaactca gagatatcgc cacgcggcgc
    22201 gttgatcgcc tgttccatta cacccaggat gtgcagacgc gcacctttag cctggttcag
    22261 tgcaacctgc atgatctctt tggtgatacc ttcaattttg atatccatct gcaatgcaga
    22321 gataccgtcg cgggaacccg caactttgaa gtccatatcg cccaggtgat cttcgtcgcc
    22381 caaaatgtca gacagtacaa cgtagttgtc gccttctttc accagaccca ttgcgatacc
    22441 cgcaacggca gctttgattg gcacacctgc gtccatcagc gccagagacg cgccgcacac
    22501 ggaagccata gaagaagaac cgttggattc ggtgatttca gacactacac gtacggtgta
    22561 cgggaatttg tccatatccg gcatgactgc cagcacgccg cgcttcgcca gacgaccgtg
    22621 accaatttca cgacgcttcg gagaaccgac catgccggtt tcgcctacgg agtacggagg
    22681 gaagttgtag tggaacagga aggtatcggt acgttcgccc atcagttcat caagaacctg
    22741 cgcgtcacga gcagtaccca gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa
    22801 cagcgcagaa ccgtgagtac gcggcagcac accagtacgc acatccagac cacggatcat
    22861 atctttttca cgaccgtcga tacgcggttc gcctgccagt acgcggctac gaacaacatt
    22921 tttctcgata gcgtgcagaa tttcacccag ttcgttttcg tccagggttt cgtcttcagc
    22981 aagcagtgtc gcgatggttt cagatttgat gacatcaacc tgcgcataac gctcttgttt
    23041 gtcggtgatg cggtaagcat cgctcagacg agcttcagcc agtgcagcaa cgcgcgcgtt
    23101 cagcgcttcg tttaccggct ccggctgcca gtcccaacgc ggtttaccgg cttctttcac
    23161 cagttcattg atgttctgaa taacaacctg ctgttgttca tgaccgaaca ctactgcgcc
    23221 cagcatctgg tcttcgctca gcagttcagc ttcagattca accatcagta cagcggcttc
    23281 agtacccgca acaaccagat ccagtttgct ctctttcagc tcgtcctgtg tcgggttcag
    23341 tacgtactgg tcattgatgt aacctacgcg ggcagcacca atcggaccat tgaacggaat
    23401 accagacaga gacagtgctg cggaagcacc aatcatcgcg acgatatccg ggttaacctg
    23461 cgggttaaca gaaaccacgg tggcgataac ctgaacttcg ttgacgaagc cttccgggaa
    23521 cagcgggcga atcgggcggt caatcagacg cgcgatcagg gtttcgcctt cgcttggacg
    23581 accttcacga cggaagaagc tacccgggat acgaccagca gcgtaggtac gctcctgata
    23641 gttaacggtc agtgggaaga agtcctgacc tggtttggct tttttctggc caacaacggt
    23701 aacaaatacc gcggtgtcat ccatgctaac cataacagcg gcagtagcct gacgagccat
    23761 catgccggtt tccagagtca cggtgtgttg gccgtactgg aatttacgaa cgatcggatt
    23821 aagcaatgta atatcctttc tctttcttag acagtacctt acggcactgg tgttaatacc
    23881 cgatcttctg cgcatcctcg cgactaatga caaccctaac ccagctctat gtgggtaaag
    23941 cctctcatta gccgcgcgaa cctctgcaac ggaagatcat tcatagcaac aatacattag
    24001 tttccagtga attgctgccg tcagcttgaa aaaaggggcc actcaggccc ccttttctga
    24061 aactcgcaag aattagcgac gcagacccag gcgctcgatg agctgggtgt aacgtgctac
    24121 gtctttacgt ttcaggtagt cgagcagttt acgacgctga gaaaccatgc gcagcagacc
    24181 acgacggctg tggtgatctt ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg
    24241 tgcagtcagc agtgctacct gaacttcggt agaaccggtg tcgtttgcgt cacgaccaaa
    24301 ctcagaaacg attttagctg ttgcttcagt acttagagac attttaaaac tccaaagtat
    24361 atagaatgaa aggacgccga tctctaattc agcgatccca gtgtacgtta cgcaaagtgt
    24421 taaacaattt acgcgacgtt aagcggcagt attctactcg tagcgacctg ttatcgcaag
    24481 acggttaaca ttacgccggg tattcaacca ccagacgacg aggcgcaacg cggccttcat
    24541 cgtcaatttc gcccataccg ataaacttgc cgttctcacc ttccgtaacg cgaaccagtc
    24601 cttccagcgg cgcgccagat gtacgaaccg ggttaccatt tttgaagtaa acagaagacg
    24661 ttaacggaag attcaccacc ggatagtccg aagctggact gtccattggc atcagtaatg
    24721 gatcaagtaa ctccgcagcc ggaatatcct gctgttcagc ttgctcaaca agttcacgca
    24781 ggtgctccag ggtcaccatc cgttcaaccg gatatttact taccgccaga cggcgcaggt
    24841 aaataacatg cgcgccacag ccgagtttct cacccaggtc gtcaatgata gtgcggatat
    24901 aagtgccttt tgagcagtga atttccagct ccagctcatt gccttcatgg cgaataaaca
    24961 gcaattcata aacggtaatc ggacgcgctt cacgcggaac ttcaatgccc tgacgcgcat
    25021 attcgtacag ttttttgccc tggtatttca gtgctgaata catcgatggg atctgttcga
    25081 tatcgccacg gaaagtatcc agtgccgccg ccagttgttc tgcactaaag gttaccggac
    25141 gctcttcaac gatttgtccg tcggcgtcag aagtatcggt acgctgtcca agacgcgcaa
    25201 tgacccgata gcgtttgtcg gagtccagca gatactggga aaacttcgtc gcttccccga
    25261 ggcaaatcgg caacatgccg gtcgccagcg ggtccagcgc accggtatga ccggcacggt
    25321 tggcgttata aatccgtttc actttttgca gcgcatcgtt gctggacata ccctgaggtt
    25381 tatccagcaa caaaacgccg ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc
    25441 ctccttgctg tcgtccgggt taacacgacg ttcttcgtca tgtttgacca cgctggtcac
    25501 caggtttgac atgcgcatcc cttcaaccag agagttgtcg tagaagaagg tcagttccgg
    25561 cacgatacgc aggcgcatcg ctttccccag caggctgcgg atgaaaccag aagcttcttg
    25621 caacgctttg atgcccgctt taaccgcgtc ttcatctttg tcgttgagga acgtcacata
    25681 tactttggca tacgccaggt cgcgagacat ttcgacaccg gaaacggttg tcatcatgcc
    25741 cagacgagga tctttaattt cacgctgcag gatgagagcg atctcttttt gcatttcctg
    25801 cgctacgcgc tgcgggcgac caaattcttt cgccataata aattctcctg acaaaaaagg
    25861 ggctgttagc ccctttttaa aaattaattt caggtggaag ggctgttcac gttggcctga
    25921 taagacgcgc cagcgtcaca tcaggcaatc catgccggat gcggcgtaaa cgccttatcc
    25981 cgcatggaac cctaaaaacc ttaagcgatg gtacgttgga tctcgatgat ttcgaatact
    26041 tcgatcacat cgccagtgcg gacgtcgttg tagttcttaa cgccgatacc acattccata
    26101 ccgttacgga cttcgttaac gtcatccttg aagcggcgca gggactccag ctcgccttca
    26161 tagataacca cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg
    26221 gtaaccatac agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc
    26281 gccagaccga taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc
    26341 acttcgtcaa tcaggttata gatgacggag tagtaacgca gatccaggct ttccgcttca
    26401 atcactttac gtgcagaggc atcagcacgt acgttaaagc caaccaggat ggcgttggac
    26461 gccgcagcca gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc
    26521 actttaactt cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc
    26581 tgtacgtctg ccttcaggac gatattcact tcgtgaactt cgccttcggt catgttggcg
    26641 aacatgttct cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc
    26701 tgacgataga gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca
    26761 cccgcagccg gaacaccgga caagccgagg atttccaccg gaatggacgg acccgcttcc
    26821 agcacttcct gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac
    26881 agaacgatat cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca
    26941 cgacctttat cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct
    27001 ttcagctcca gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gataccggta
    27061 cccgctttcg cagatacgtg tacgaactgg ctttcaccgc cccactcttc cggcaggatg
    27121 ccgtactggg agagttcgtt cttaacgcga tccggatcgg cttctggttt atcgatcttg
    27181 ttcactgcaa ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc
    27241 tgcggcatta cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca
    27301 ccacgtgcac gcattgaagt aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg
    27361 ccgttttcag tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca
    27421 gaggccactt tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga
    27481 cccatgatgg tcacaaccgg agcgcgcggt tcagccgcag cccccgtgtc acggtcgctc
    27541 attaccgcct cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca
    27601 gcaaccagct gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt
    27661 ttcatcatcg ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca
    27721 acggtgatag tttcgccgat cacaacgtca cggttaacgg cctgagcagg cttctggaag
    27781 ccttgctgca gcgaagaacc tttacgtttt ccgcccttac cgccacgtac tgctgcgcgt
    27841 gcttcttcac gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct
    27901 ttcgcgttac gaccacggcc acggccgcct tcgacttcac gatcgctttc gtcttctgcc
    27961 tggcgagcat gttgagaagt agtgacgtga taatcgctgg aatcctcagt cggttccgcg
    28021 ttatcagtcc atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct
    28081 tcttcttcga gtttacgacg cgcttcttct tcggctttac gcttgagctc tgcagcttcc
    28141 tgctcacggc gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc
    28201 actttgtctt tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt
    28261 tctgcggcct cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca
    28321 cgacgggctt gctcttccgc ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc
    28381 tcttgcggat cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg
    28441 ctttttccac cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat
    28501 ttgtccgggc ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgca
    28561 gacacagagt cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt
    28621 tccacggagg tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc
    28681 cttcctgcta cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata
    28741 atcagtgctc cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc
    28801 tgttcggcga gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgtcaaatca
    28861 cgatctaccc cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct
    28921 tcctgggcct gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg
    28981 gtcggctcat caaggccttc gatttccaac agctctttca tcggcacata ggccagttct
    29041 tccagcgtcg agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga
    29101 tatttggtga aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg
    29161 tcaacggtca tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga
    29221 ccgttacggc caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt
    29281 ttatcttcat ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac
    29341 tgcgccgggt tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa
    29401 accgcctgaa cacgcgcgcc acgcatacct acgcaagcac ctaccggatc gatacgttta
    29461 tcgttggttt tcaccgcgat tttcgcacga gaacccgggt cgcgagccgc tgctttaatt
    29521 tcaatcactt cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc
    29581 ggcttggaac gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaataaagc
    29641 acgccacgaa cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg
    29701 atcacggctt cagcgttgtt gcccagatcc agagagatgt tgtcgcggtt tacttttttc
    29761 accacgccgg tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt
    29821 tcggcttcac gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca
    29881 aaggtaacag actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca
    29941 taacgtgcgg cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac
    30001 cagcgacgga aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc
    30061 tcttgttcat attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc
    30121 ttctcgcgag gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg
    30181 ttcatcgcgg gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc
    30241 tggatattac tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca
    30301 ccgtctaccg ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga
    30361 accagagtca cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga
    30421 tcgagacccg gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc
    30481 agcacagcac ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca
    30541 ctatcaatat agatgcgcag tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt
    30601 tcaaaaccca gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg
    30661 gacaagccca cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga
    30721 taacaaaaaa ccccgataaa tcggggcttt atataactga accctataac cgcaactgcg
    30781 gtctggagca ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac
    30841 agtatatttg aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg
    30901 accttcgggt tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaacgtgg
    30961 caaattctac tcgttttggg taaaaaatgc aaatactgct gggatttggt gtaccgagac
    31021 gggacgtaaa atctgcaggc attatagtga tccacgccac attttgtcaa cgtttattgc
    31081 taatcacgtg aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg
    31141 atccccctta agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctctgataaa
    31201 agatgattaa atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag
    31261 gctcatcatc agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc
    31321 cggtaggtca acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc
    31381 tgtggatgcg acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag
    31441 acgaagagga ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac
    31501 gtctgatcga ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg
    31561 catttcataa caccaccggt ggcctgacct atttcaacac gacgccgctg ggccgcgccg
    31621 tgactggcac catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg
    31681 gcagcaccta taaaggaaac gatatcgaac gtttctatcg ttacggtctg ctgaccaatg
    31741 ctgaactgca gatttacaaa ccgtggctcg atactgactt tatcgatgaa ctgggcggtc
    31801 gtcatgaaat gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa
    31861 aagcttactc cacggactcc aacatgcttg gtgcaacgca cgaagctaag gatctggaat
    31921 acctcaactc cagcgtcaaa atcgtcaacc cgattatggg cgtgaagttc tgggatgaga
    31981 gcgtgaagat cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtggcgt
    32041 tgaacggtaa aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg
    32101 gtcgtcacgg cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc
    32161 gtggtattta cgaagctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga
    32221 ccggtattca caacgaagac accattgagc agtatcatgc acatggtcga caattgggcc
    32281 gtctgctgta ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc
    32341 aacgctgggt tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg
    32401 attattcaat cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga
    32461 tggaaaaagg cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta
    32521 atctggatat cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct
    32581 cctcctctgc cacttcaggc gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat
    32641 tcgacatcaa ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt
    32701 tctgagcatc acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt
    32761 atagtgtgca atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca
    32821 cgcattaata aggtaattat tgtaagctgt agaaaagatg ttgttttccg ttgtgcgatc
    32881 cacaccatcc ccaagaacgg ggctatacca gataaacatc ggaacatgat atgcctgctg
    32941 gctggcctcc ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc
    33001 gtggtcggca aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc
    33061 tagcaaacta tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg
    33121 ttgaaacacg gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa
    33181 atgaagaaca atcagtttct tttgctgtgt attttgctgt aatgcttgcg ataaatgcgg
    33241 caacaacaat tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact
    33301 ggtaactgct gtaccattct gccgaaaagc ggattgcgaa cttagccaga aagtctgaaa
    33361 tcctgcttga ttagccatat taataatgtt gtcggggtaa ttatgaatgt catggctcat
    33421 aacagaatca gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg caccgcttat
    33481 tgcttgatta aacagtttga tctgttttct ttgtgcttcg acttgcggtg tcgtagagcg
    33541 tgtatatcca tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta
    33601 ggtatcaata cctgtatccc tgactgataa ttgaaaatac ggcactgtgt ttgcaattga
    33661 gagtaatctt tgatgctctt tcgctgctaa agcaaaataa ttgagattga aaaacggcgt
    33721 ataggtagca aatcgcgacg ctagtatata tggactgaac gcatttttat ttttggcatc
    33781 tttataagca aattgacaag cggaaaataa acttcccgaa atgacaatca gcaataatat
    33841 tcctgtcact ttttttgtcg gcaaggaaac atcatatttt attattacag ccaaaaaaag
    33901 aagggataaa aaggcaaaag cacatagata agggctatac attcccagca ttttgacaac
    33961 ttcatccgga tcactctgca gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa
    34021 ggtacaccat gaatagaggc taatactcat gtctgccgca gtcagtaaca caaatggtgc
    34081 ggcaacaagc aggcgaagcg gaagggatga tatacgttta acaaccagga tggtcagaac
    34141 gaagaacagc aatcgctgaa ccatatgcgc tccggaagag gcgactaagt tcgtaatacc
    34201 aaaaacaata acactaagat acaacaacca atgagattta aaacttctag caaatttgtt
    34261 gaatactgtc atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgtgc
    34321 aattttatat cagacatgtt tttcaactct gatccatatc agaacgaaac actgttagtt
    34381 ttttcttttt aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg
    34441 tctctaaaca ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt
    34501 tatcgagaag taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc
    34561 aattatgtca gcagtaggga aaagtttata acgaaggcgt accggcacca tcgtttcaag
    34621 gtaccagcta cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga
    34681 ccaggaccaa aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag
    34741 gacgggactt gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc
    34801 aattccacca cctcggcacg gaaactactt tttagttcgg gatatcgctg gtcggcttag
    34861 ccggagcagc tggctgagtt tgttcggttt tcgccggcgc actcaggttt tcccattcgc
    34921 tacctttatt ggttttgttg ctattgatgt tacccagcac cagactgatg atgaagaata
    34981 acgttgccag caacgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca
    35041 gcgtagcgga agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca
    35101 gcatgatcag accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt
    35161 acataatcaa cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg
    35221 gagtttttaa tctttcccac tgaagcgggt gtgaatacta accaaagcga atgaccttcg
    35281 caagggcaat tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc
    35341 gccgagaaaa aaggcgacaa tcgccgcctt tttagccagt tatctaacgc tttaaacggc
    35401 ttttactgca tcggcgatgc ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc
    35461 caccatcacg cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct
    35521 acccagcgcc gcttcaactt ctgcggtcac agctttgact gattcatgct ccagcggatc
    35581 gccgctacct gcggtgtaac gcacgtttac caggatttgc gggaacattt tcatgccgct
    35641 gcaaaggtcg tgcaggctca tatggttacg cgccatcgcc gccagcacct gcaaaccagc
    35701 aacgatgccg tcaccggtag tagttttatc cagcaggatc acatgaccgg aattctctgc
    35761 accgatacgc cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc
    35821 gcgcgcaaat ggaataccta actgtttcag cgccagttca agccccatgt tgctcatcaa
    35881 tgtacccaca gcgccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata
    35941 catgatctga tcgccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc
    36001 gtcgccgtcg aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc
    36061 gcgaacgtcg gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat
    36121 agcgataacg ttcgccccga gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc
    36181 gtttgcacaa tccaccacaa tcttcagttc actgaggcta agttcgttcg ggaacgtggc
    36241 tttgcaaaac tcgatatagc gacccgcggc atcaacgata cggctggctt tacccagttc
    36301 tgccgaatca acgcagctga tctccttttc catttccgct tcgatggcct cttctaccgc
    36361 atccggcagt ttggtgccgt cgatagagaa gaatttaatg ccattatcgt agaacgggtt
    36421 atgcgatgca gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac
    36481 ggccggtgtt ggcatcgggc cagtgaagag tgcggaaagg cccgcagccg ccagccccgc
    36541 ttccagtgct gactccagca tatagccaga aatacgcgtg tctttaccaa taataatctt
    36601 acgggagccg tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc
    36661 aggtgtgatc ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg
    36721 attactcata gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt
    36781 ctacggtttc tttgacgtca tgaacacgaa tgatgtgcgc gccttgcatt gcggcaatga
    36841 ccgcacaggc cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa
    36901 tcatcgattt tcgtgacata cccaccaaca gcggcaggtt gaaatggtga aattcagcca
    36961 ggcgcgccag taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga
    37021 gcagcaattt ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt
    37081 agcgattcac ttctgcaaag acatcgtcat acttcggggc ttcctgcatg gtttttggat
    37141 ttccctgcat atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc
    37201 caggttcgga aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac
    37261 ggatgacttc tggtttggat gtatcgaccg agatccagac ttcgaagcgt tgagcaattg
    37321 cctcaaccac aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg
    37381 ggcgcgtgga ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg
    37441 catgtttcac cgcatctatc agcgagttat gcgtgccacc atctgaaaag gaatcaggcg
    37501 tgacgttgag gatccccatt acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa
    37561 agagtttcat aatgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc
    37621 ggggtttttg gtacaaatac agtcatctta tgcgggaact tacttgtcgc ctaactgctc
    37681 tgacatggtg ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg
    37741 actaccattg tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg
    37801 tacatcgcga cgtgccatca ggtcatcaat ctgtggtgcg tcgatagtct catatttcat
    37861 gagagcatct ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg
    37921 attatagtta cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc
    37981 ggacatatgt ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc
    38041 gtacagcagt ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgtgccag
    38101 gttggtcgca actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat
    38161 gatctcttct gccagacgac caccgtacag agtagaaatc tggctttcca gtttctgacg
    38221 gctggcgctg attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg
    38281 tgggataatc gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc
    38341 atgacccgct tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg
    38401 acgttccgca cccatcatga ttttgtcttt cgctttctcg aattcaacca tcgacacaac
    38461 gcgtttgttg ccacgagcag cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc
    38521 accggagaaa ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg
    38581 tacgcgacgc atgtgaacct tcaggatctg ctcacgaccg cgaacatctg gcaagccaac
    38641 cacaacctga cggtcgaaac ggccaggacg cagcagggcc gggtcgagaa cgtccggacg
    38701 gttagtcgcg gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag
    38761 catctggttc agagtctgtt cacgttcatc gtgaccaccg cccagacctg cgccacgctg
    38821 gcggcctacg gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg
    38881 ttcgaacatg tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga
    38941 accggagata gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt
    39001 tttaccggta cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt
    39061 ctggaagcgg ctcggctcgc gcagatactc aaccagttca gcaacttctt cttttgcttc
    39121 gtcgcagccc gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc
    39181 tttgctctta ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa
    39241 gaagatccag acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag
    39301 caggcttggt tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc
    39361 cagtaatttc ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt
    39421 aacgttgatt tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg
    39481 taggaaggta gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa
    39541 tactgacatc agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact
    39601 caagggatta acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca
    39661 gcatctttca aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat
    39721 atacacttcc cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa
    39781 cagggagcga atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac
    39841 aaaactgcca cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata
    39901 catggcacgg gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat
    39961 gacaacctgg actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc
    40021 acgaaaatcg ccctgaagaa agtccacacc aacgatagga tccataggta aaagatcgca
    40081 agcgatgatg cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg
    40141 agcagcacca aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat
    40201 ttcatcaagt ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata
    40261 tttatcgcta aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt
    40321 catttaactt tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct
    40381 atttggtgat atatgggaga tggcggtaga atgacccgtt ttcaatccca acgtaagcaa
    40441 aaaatacgat gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc
    40501 tcaagccagt tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg
    40561 aacaagcgtt agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa
    40621 caaaaacctt gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca
    40681 tcggtaaaac gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct
    40741 aagattatcc taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg
    40801 agagcaaaat gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc
    40861 tttacaatac atcaacatct tgagtattgg gtaattctta aaggtattcc accttaatta
    40921 cttcaaattc tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc
    40981 cgatcaggcc acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt
    41041 cgtcatcgcc aacgatgcga taagtctgtt cttcgtcaga atcgagattc agcaccgtta
    41101 cggtagcacc aaaaataacg cgcccattgt tgggcatttt ggtgacatca atcacctgcg
    41161 cgttcgacag cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac
    41221 gagctgcgtg gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag
    41281 cagcaatgat ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt
    41341 cagcgccgcg taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga
    41401 tggggcaagt tttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga
    41461 ccagaagcaa aaaaataccg acccgggtac aagtcccagg tcagctacaa ttcacatttt
    41521 gatagtcatt ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc
    41581 cgtagtatga cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta
    41641 tcatcggatt gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca
    41701 ttactcaact ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg
    41761 cccccgctat tgattaccac agtcagcaga tggcgctgcc cgccagtacc cagaaagtga
    41821 ttactgcgct ggcggcgttg attcaactcg gccccgattt tcgtttcacc acgacgcttg
    41881 aaaccaaagg caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg
    41941 ccgatccgac gttaaaacgt caagatattc gcaatatggt cgcgactttg aaaaaatctg
    42001 gcgtcaacca aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata
    42061 aagcccccgg ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgctg
    42121 ccatagttga ccgcaactgt ttctccgtct cgctctacag tgccccaaaa cctggtgata
    42181 tggcttttat acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc
    42241 tcccccgtgg ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga
    42301 accgctttac gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg
    42361 ccgtgcagga tggagccagc tatgccgggg caattctgaa agatgagtta aaacaggcag
    42421 gtatcacctg gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag
    42481 ttgccagtaa acagtcggcc ccgttgcacg atctgcttaa gattatgctg aaaaagtcgg
    42541 acaacatgat cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtgcctg
    42601 gaacatggcg ggccgggtcg gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg
    42661 atattggaaa caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc
    42721 ccgccaccat gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta
    42781 tctccatgct gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc
    42841 aggcgggcgt ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc
    42901 tggcgggatt cattaccaca gcgagcgggc aacgaatggc gtttgtacaa tatctttctg
    42961 gctatgcagt agaaccggcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa
    43021 gccgtttgta taaagatatt tatcagaata attagtctaa agagaaaccc cggcacatgg
    43081 ccggggtttt gctgattaac gcttgtaaat aaactcaacg ccttcttcgt cgtcttcgtc
    43141 ccaatcgtca tcccagtctt catcatcctc ttcagcaatc tcttcaagct gctggcgatg
    43201 ataatcatcc cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac
    43261 cgggttttca atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccgct
    43321 cgccgcagag atcagataat atttatcttc ccagcccagc gcctcagcga tcgctttcgc
    43381 tttctcttcg gcttctacct tatccagcag atcgatcttg ttgaagacta accaacgcgg
    43441 tttcgccgcc agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc
    43501 aaccggatcg gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg
    43561 ttccaggtgc ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag
    43621 tcctggaata tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact
    43681 tggcaccaga gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg
    43741 aataaaggtc gatttacccg catttggcat ccccaacata ccgacgtcag ccagcagcat
    43801 cagctccagc agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt
    43861 acggttaact gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac
    43921 cagcagacgc tgaccgtgtt tggtcatatc gcccatggtt tcaccagtac cctggtcgat
    43981 tacacgcgta cctaccggca ctttaattgt gacgtcttta ccgcgcttac cggtacagtc
    44041 gcggcttgcg ccattctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat
    44101 aagcgtgttc aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc
    44161 gccatccggg ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc
    44221 gccatcacct gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt
    44281 aaatcattcg cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca
    44341 aatacgatga ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata
    44401 acctaaaagg tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc
    44461 tgaaaaaaac agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg
    44521 ccagcgagcc atcgcttccg ccagggcgcc atatcctacc aaggtattca gtccgcaaaa
    44581 aattaaacat gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg
    44641 gaagagcgca attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg
    44701 acgcaataaa accttttgcg ccacgccata actcacccaa accgtcgcag caccgacccc
    44761 aaagataact ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt
    44821 aaaaaacatc accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat
    44881 tttctctttc aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat
    44941 cacctgcgaa gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc
    45001 aaacagcccg gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg
    45061 taatggcggc aacctcttct tcaccgcaag aatggccccc aggccaatac tcgccatcaa
    45121 gaaacggtaa aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat
    45181 tggcaacgcc ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg
    45241 ctgcttcata ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat
    45301 ttttaccggt tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac
    45361 cgggacgcga aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt
    45421 aacttcgaat ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc
    45481 tacgttagcg ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag
    45541 aacggattcg ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt
    45601 acgtgtggag ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg
    45661 ctgatgccag taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag
    45721 tgtttacgac gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact
    45781 tcagctttga ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt
    45841 gcgatcatca gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc
    45901 aggcgaacgg tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg
    45961 tacatataaa actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata
    46021 ttcacaatag ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat
    46081 acacgaagaa aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac
    46141 aatcacccta tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg
    46201 gggcgaaaag cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag
    46261 atatggcggg tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc caactgatca
    46321 atcagttagg ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg
    46381 tactggctgc acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga
    46441 tcgagtttat ccacacggcg actctgctac acgacgacgt tgtggatgaa tcagatatgc
    46501 gcaggggtaa agctaccgcc aacgccgcat ttggcaatgc cgccagcgtg ctggtaggcg
    46561 attttattta tacccgcgct ttccagatga tgaccagcct cggttcgctc aaagtgctgg
    46621 aagtcatgtc agaagccgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaatg
    46681 ttaacgatcc ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc
    46741 gtctgtttga ggctgccgcg cagtgttccg ggattctggc tggctgtacg ccggaggagg
    46801 agaaaggcct gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt
    46861 tactcgatta caatgccgat ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg
    46921 aaggtaaacc gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac
    46981 agatgatccg taccgccatc gaacagggta acggtcgcca tcttctggaa ccggttctgg
    47041 aagcaatgaa cgcttgtgga tctcttgaat ggacgcgtca gcgcgccgag gaagaagcag
    47101 acaaagccat cgcagcgtta caggtgctcc cggacacccc ttggcgagaa gcactcatcg
    47161 gcctcgcgca catcgctgtt caacgcgatc gttaatcccc tcccctcatc ccgcgcagtg
    47221 cgcgggatga ttccagtaaa ctccataaga atacttattc agcgtaataa tgcactgaaa
    47281 aaccagtgta tacatcttta tatattctga atattcacac tctttacagg aactttttag
    47341 agcaatcggc catcaggagt atagtgatgc tcgacagaag aagtgttctg aatgaaagcg
    47401 actaacttaa ggagtgagga aagatggaaa gtaatttcat tgactggcat cccgctgaca
    47461 tcattgcggg tttgcgcaag aagggaacgt caatggcggc ggagtctcgc agaaatggtt
    47521 tgagttcctc aacgctggcg aacgcattat cgcgcccatg gccgaaagga gagatgatta
    47581 ttgcgaaggc cctgggaact gacccctggg ttatctggcc atcacgctac catgatccgc
    47641 agacccatga gtttatcgac agaacgcagt tgatgcgtag ctacactaaa ccgaaaaaat
    47701 gagtggtctg gcggtagccc cgcgaacggg gctgccagct ttcagacgat tactcgcctt
    47761 tcacacgctc aatatttgca cctaaagcgc gcagtttgtc ttcaatgcgt tcgtagccac
    47821 gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc cgcaatacag ccagccagca
    47881 ccaggcttgc tgatgcacgc agatcggttg ccataacctg tgcgccagaa agtttttcaa
    47941 caccgtgaca aataacggta ttgctttcga tttcggcgtg tgcgcccata cggctcagct
    48001 ctggcacatg cataaagcgg ttttcaaaga ccgtttcggt gatgaatcca gtcccttctg
    48061 ccaccaggtt caacagcgtg aactgggcct gcatatcggt cgggaatgcc ggatgcggcg
    48121 cggtacgtac gttaacagcc ttcggacgtt tgccatgcat atccaggcta atccagtctt
    48181 cgccgacttc gatgtccgct ccagcatcac gcagtttcgc cagcacggcg tcgagagtat
    48241 ctggctgcgc gttacggcag ataattttgc cgcgagaaat cgccgccgcc accaggaaag
    48301 taccggtttc gatacgatcc ggtagcacgc gatagacacc gccgcctaaa cgttccacac
    48361 cttcgatgac gatacgatcg gtgccctgac cgctaatttt cgcacccagc gtaatcagga
    48421 agttcgcggt atcgacgatt tccggttcac gcgctgcatt ttcaataatc gtggtgcctt
    48481 ccgccagggt tgcagcacac atgatggtca ccgttgcgcc aacgctgact ttatccatca
    48541 cgatatgtgc gcctttcaga cgaccatcga cggaagcttt aacgtaacct tcttccagtt
    48601 tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag atcaaccgga cgcgcaccga
    48661 tcgtacaacc gccaggtagt gaaacttgcc cctgaccaaa gcgcgctacc agcggcccca
    48721 gtgcccagat agaagcacgc atggttttaa ccagatcgta aggtgcgcag aatacattaa
    48781 cgtcgcgggc atcaatatgc acagaaccat tacgttctac tttcgcaccc agctggctta
    48841 gcagcttcat tgatgtatcg acgtctttca gtttcgggac gttctggatc tctaccggtt
    48901 cttccgccag tagagcggca aaaaggatag gcagagcagc atttttagcg ccggaaattg
    48961 tgacttcgcc ctggagcttc gttggcccct gaacacgaaa tttatccatt tagtttgttc
    49021 tcagttaaca attcatatcc gctaccggcg aatcgcccat agctcaaaag ccgttcagtt
    49081 tgcgatcgcg cgcccactcc gcaggggtat acgctttgat cgacacagca tgaatgcggt
    49141 tatccgcaat atattccatc agcggaccat agaccgtctg ctgtttttta acccgactca
    49201 tgccgtcaaa caactcaccc acggcaataa cctgaaagtg gctgccatcg ccggaaacgt
    49261 ggacttcctg gagggagaga gcgttcatca acacgctctg aatttcatta ttttccatgg
    49321 gatcttcaat catcagttaa taaaccagcg aaacatctta gagcaaagtt gcgctggcat
    49381 aaataagcaa aaagcctcgc tgataaatca gacaaggctc gacttgcagg caggtttgcc
    49441 ggacaggcgg ttaacgccat atccggcctg aaaaaattta acgaggcagc acatcagcag
    49501 gcaaattata caatttcgcc agggtataca ctttgtcgtt taccccctga agcgtcacat
    49561 tgttgccctg ctttttcgcc agttcgataa gatggagcag cagtgccagt ccccccgtat
    49621 ccacgcggga cacacggcta agatcgatgc aggtaacccc cttcaccgct tcctcacgca
    49681 tttcccaaag cggtagcaga acgtcctgat ccagctctcc ggataacgcc agcgtgtcac
    49741 ccgtctgcat ccagctcagt gactcgctca ttattttttc tcttccagag tgattttctg
    49801 ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata cctttggtac gcagcagcgt
    49861 tccccactcg ttttgtttgg tggtgatcat actgacgcct tcagcaatca tgtcgtaagc
    49921 ctgccagttg cccgtctggg agtttttacg ccactggaag tccagacgca ccggcgggcg
    49981 gccattcggg tcaataatgg taacgcgaat aggcacaatg gttttatcgc ccagcggctg
    50041 ttctggcgca atctgatagg tttgaccgtg atacatcgcc agcgcctgac cgtaagcctg
    50101 cttcaggtac tcacggaaag cggcaaagta ggcttcacgt tgagctgggg tcgcactctt
    50161 gtaatactgg cccagcacca gcgcaccggc gtatttcacc tgtacgtatg gcagcagttc
    50221 ctgatcaaca atggtacgca gataatccgg gttggcccga atttgcggtt gctcattctt
    50281 caggcgatcg aacgttttct gcgccgcctc gtccatcagc ttatacggat tggtctggtc
    50341 tgccgcggtt gccgcactca gcggtgcaat caccagcaaa gcgaccatca ttaaacgttt
    50401 aaacatgcgt cggttctcct gaaattattt cgttgtaccc acaggttcag tggtttcatt
    50461 attacctggc gcagcagctg gcgcatcgcc actattctta ttgtcatcgc ctttactacc
    50521 gtaaaggaac tgaccaatga gatcttccag caccatcgca gacttggtgt cctgaattgt
    50581 atcgccatcc ttcaggatag cagtccccag ttccgggtct tcaaaaccga cgtttaatgc
    50641 cagatattgt tcccccagca ggccggaagt acgaatgctc agcgaactgg tatcaggaat
    50701 gtggttataa cgttgttcaa tttccagcgt tacgcgcggc agataggttt tcgggtccag
    50761 cgtaatatcc gccacccggc ccacaacaac gccaccaata ctgaccggag agcgcgcttt
    50821 caggccgcca atgttatcga acgtcgcata aagcgtatag gtcggttcag tacgtatgga
    50881 cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc agcagtgctg ctaataaaaa
    50941 gatacccacc caaatttcat tttttttcgt ttgcatgaac tcaattccca aacatcaatg
    51001 cggtcagcac aaaatccagc cccagaacag ccagagacga gtggacaacg gtgcgagtgg
    51061 ttgcccggct aatcccggca gacgtcggga tggcgtcgta gccgttaaac aacgaaatcc
    51121 acgtcaccgt gatggcgaac accacgctct taatcagaca gttgaccaga tccatacgcc
    51181 agtcgacggc attttgcatt gccgaccaga agaacccgct atcaatgcct ttccaactga
    51241 cgccgaccag cgatccgccc cagatcccca cggcgacgaa gataaccgtc aacagtggta
    51301 atgaaataac cccagcccag aaacggggag aaataacccg acgcagcgga tccaccgcca
    51361 tcatctccat actggagagt tgctctgtag cgcgcatcag gccgatttct gcggttagcg
    51421 ccgaaccagc acgcccggca aacaacaacg cggcaaccac cggccccagt tcacgcagta
    51481 gcgataacgc caccagcata cccagactgg tttccgcact ataagtggtc agaaccagat
    51541 aaccttgcag ccccaacacc attccgatga acacgccaga aaccacaata atcagcatcg
    51601 acaggacgcc gacattatag agctggcgca ccagcagcgg cgcatgtttg cgaaattccg
    51661 gtttgccgac cagcgcattg aataacatta acccggcccg cccgaacgtt ctcagggttt
    51721 taatcccttt atgtccgagc gacgccagcg catttaacag catgagtggc ttaactccct
    51781 ggtaaaagat cagcgtgata atcgccggca ggatagcgga acggaacagg cccgtcagct
    51841 atcccgtcca gaaactgacg tacgcgcgga tcaggattcg cctgcaacgc ctgggcactg
    51901 ccatgagcga caattttttt gtccgccagg atccaggcgt gatccgcaat acttaacact
    51961 tccggcacat cgtgagaaac caccacacaa gtcacgccca gcgcgctgtt cagctcagaa
    52021 atcagcttca ccagtacgcc catggtgatg ggatcttgcc caacaaaggg ctcatcaaac
    52081 atgatgagat ccggctccag cgcaatcgca cgcgccagcg ctgcacgccg cgccatccca
    52141 ccggaaagtt cagaaggcat tagtttagcc gctccacgca gccccacggc ctccagcttc
    52201 atcatcaccg tactatgcaa caatggcgcg ggaagttggg tatgttcgcg cagtggatag
    52261 gcgacgttgt caaatacgtt catatccgtg aacaacgccc cggactgaaa taacatgctc
    52321 atccgtttgc gcactgtata caggcgcgaa cgagacatcg ccggaatatt ctcaccatcg
    52381 aaaaggatct caccatgatc tggtgcgatt tgcccgccaa tcagacggag tagcgtcgtt
    52441 ttaccgatgc ccgatggccc catgatcgcc gtgatcttcc ctcgcggcac ggtcagggaa
    52501 atattatcga agatgcagcg attgccacgc gtaaaactga catcgcgcat atcgactaaa
    52561 ttcgccacag actgctccat aattcaccct tcgtcttgcg ttgattttct aagcatggcg
    52621 ctcaatttaa ccttgaaccc aacatattta cagaatatta cccgccgtgg ttagcgaaag
    52681 ctggcatttg ttttactttt tagccgcata aagtcaaaat taagaatccg ttacggcttt
    52741 ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa ggacgttaga tgcttttagc
    52801 tacggcactg ttaattgttg gtttactttt ggtcgtttac agtgccgacc gcctggtttt
    52861 tgccgcgtct attctttgcc gaacctttgg catcccgccg ctgatcatcg gcatgacggt
    52921 ggtcagtatt ggcacatcgt taccagaaat catcgtctcg cttgccgcgt ctctgcacga
    52981 acaacgcgat ttagccgttg gtacagccct cggctcaaac attatcaata ttttgctgat
    53041 cctcggtctg gctgcgctgg ttcgtccttt taccgtccat tctgatgttc tacgccgtga
    53101 attaccctta atgttgttgg tcagcgtggt ggccggttcc gtactctatg acggacaact
    53161 tagtcgcagc gatggtatct ttctcctctt tctggctgtg ctatggctgc tgttcattgt
    53221 taaacttgca cgtcaggctg aacgtcaggg gactgacagc ctgaccagag agcagcttgc
    53281 agagctgccg cgtgacggcg gattgcccgt cgcgttttta tggctcggca ttgcgcttat
    53341 catcatgcca gtggccacgc ggatggtggt tgataacgcc acggtgctgg cgaattactt
    53401 tgccatcagc gagttgacga tgggtctgac ggcaattgct atcggaacca gcctgccgga
    53461 actggcaacc gcaatcgcgg gggttcgcaa aggtgaaaac gacattgctg tcggaaatat
    53521 cattggcgca aacattttta atattgtcat cgtgttgggt ttacccgcgc tgataacgcc
    53581 aggagagatt gatccactgg cgtacagtcg tgactacagc gtgatgttgc tggtgagcat
    53641 tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg ggccgtggtg taggggtatt
    53701 attaactggc ggatttatcg tatggctggc gatgttgtac tggttatcgc caatactcgt
    53761 tgaataactg gaaacgcatt atgtcgcacg tagagttaca accgggtttt gactttcagc
    53821 aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct ggcggagctt gatcaataca
    53881 tcaatcagaa tttcacgctt gcctgtgaaa agatgttctg gtgtaaaggg aaagttgtcg
    53941 tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat ggccgcaacg tttgccagca
    54001 ccggtacacc ttcatttttc gtccatcctg gtgaagccgc gcatggtgat ttaggcatgg
    54061 tcaccccaca ggatgtggtg attgctatct ctaactcagg tgaatccagc gaaatcactg
    54121 ccttaattcc agtgcttaag cgtcttcacg taccgttaat ctgcatcacc ggtcgcccgg
    54181 agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt taaagtagcg aaagaggcct
    54241 gtccgttagg gctggcaccg accagcagca ccaccgccac gctggttatg ggcgatgccc
    54301 tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga agattttgcg ctctcacacc
    54361 caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa cgatattatg catacgggcg
    54421 atgagatccc gcatgttaag aaaacggcca gtctgcgtga cgcgttactg gaagttaccc
    54481 gcaaaaatct tggtatgact gtcatttgcg atgacaatat gatgattgaa ggcatcttta
    54541 ccgacggtga tttacgccgt gtcttcgata tgggcgtgga tgttcgtcag ttaagtattg
    54601 ccgatgtgat gacgccgggg ggaatacgcg tgcgccctgg cattctggcc gttgaggcac
    54661 tgaacttaat gcagtcccgc catatcacct ccgtgatggt tgccgatggc gaccatttac
    54721 tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt agtgtaaaga ttcaaggata
    54781 aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt tacggccctg tcagcgccga
    54841 cgttatagca aaagcagaga acattcgtct gctgatcctc gacgttgatg gcgtactgtc
    54901 agatggcctg atttatatgg gcaataacgg cgaagagctg aaagcgttca atgttcgtga
    54961 cggttatggc attcgttgtg cgctcacctc tgatattgaa gtcgctatca ttaccgggcg
    55021 aaaggctaaa ctggtagaag atcgttgtgc cacattgggg atcactcact tgtatcaggg
    55081 gcagtcaaac aaactgatcg cctttagcga tctgctggaa aaactggcga ttgccccgga
    55141 aaatgtggct tatgtcggcg atgatctcat cgactggccg gtaatggaaa aagtgggttt
    55201 aagcgtcgcc gtggccgatg cgcatccact gttgatcccg cgcgccgatt acgtgacgcg
    55261 cattgctggc ggtcgtggcg cagtgcgaga agtttgcgac ttattactcc tggcgcaggg
    55321 caaactggat gaagccaaag ggcaatcgat atgagtaaag ccagacgttg ggttatcatt
    55381 gtgctatcac tggcggttct ggtgatgatc ggcattaata tggccgaaaa agacgatacc
    55441 gcccaggtgg tcgtcaacaa caatgattcc acctataaaa gcgagcatac ggacacgctc
    55501 gtctataacc cagaaggggc actaagctat cgattgattg ctcaacacgt tgaatattat
    55561 tccgatcagg ccgtttcgtg gtttacgcag ccggtactta ccacgtttga taaggataaa
    55621 atcccgacat ggtccgtaaa agcagataaa gccaagctga ccaatgaccg gatgctctat
    55681 ttatatggac acgttgaagt caacgcactt gtgccggact ctcaacttcg cagaatcacg
    55741 acggataacg cgcagatcaa tctggtgacg caggatgtta cctctgaaga ccttgtcacg
    55801 ttatacggaa caacatttaa ctccagcggt ctgaaaatgc gcggcaactt acgcagcaag
    55861 aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa ttcaaaacaa acaaactcag
    55921 ccttaatctt gtgcttgcca gctcacttct ggccgccagc attccggcat ttgccgtaac
    55981 cggagacact gatcagccga tccacattga atcggaccag caatctcttg atatgcaagg
    56041 caacgtggtt acctttaccg gtaatgtcat cgtcacccag ggcaccatca aaattaatgc
    56101 cgacaaagtg gtcgttaccc gtccgggcgg cgaacaaggt aaagaagtga ttgacggcta
    56161 cggtaaaccg gcaacgttct accagatgca ggacaacggt aaacccgttg aaggtcacgc
    56221 ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt ctgacgggta atgcttatct
    56281 gcagcaggtc gatagcaaca ttaagggcga taagatcact tacctggtga aagagcagaa
    56341 aatgcaggct ttcagcgaca aaggcaagcg tgtaaccacc gttctggtgc cgtcgcagct
    56401 gcaggacaaa aacaacaaag gccagacccc ggcacagaag aagggtaatt aattcgttat
    56461 ggcaacatta actgcaaaga accttgcaaa agcctataaa ggccgtcgcg tggtagaaga
    56521 cgtcagcctg accgtcaact ccggggaaat tgtcggtctg ctggggccaa acggtgccgg
    56581 taagaccacc actttctata tggttgttgg cattgtgccg cgcgatgcgg gcaacatcat
    56641 tattgatgat gacgatatca gtctgctgcc tctgcatgca cgcgcgcgcc gcggtatcgg
    56701 ctatctgcca caggaagcct ccattttccg tcgcctcagc gtttacgata acctgatggc
    56761 ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt gaagaccgcg cgaacgagct
    56821 gatggaagag tttcacattg agcacctgcg tgacagcatg gggcagtcac tctccggggg
    56881 tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg aatccgaaat ttattctgct
    56941 cgacgaaccg tttgccgggg ttgacccgat ctcggttatc gacattaaac gcatcattga
    57001 gcacctgcgc gacagcggcc tgggcgtgct gatcactgac cacaacgtgc gtgaaacact
    57061 ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat ttgatcgccc acggcacgcc
    57121 tacagaaatc ttacaagacg aacacgttaa gcgtgtatac cttggggaag acttcagact
    57181 ctgatagggt agaagtttgc gacgttttag caggagagta cgattctgaa catgaagcaa
    57241 ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc cacagctcca acaggcaatt
    57301 cgtctgttgc agttgtcgac gctggaactt cagcaggagc tacagcaggc gctggagagt
    57361 aatccgctgc ttgagcaaat cgacactcat gaagaaatcg acacccgcga aacgcaagac
    57421 agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag agatgccgga agagctgccg
    57481 ctcgatgcca gttgggacac catttacacc gctggtacac catccggcac cagcggtgac
    57541 tacattgacg acgagctgcc ggtctatcag ggcgaaacga cgcagacctt gcaggattac
    57601 ctgatgtggc aggtcgagct gacaccgttt tccgacactg accgcgctat tgctacctct
    57661 atcgtcgatg ccgttgatga caccggttat ctgactgtcc cgctggaaga tattctcgaa
    57721 agtatgggcg atgaagagat cgacatcgac gaagttgaag ccgtccttaa gcggatccaa
    57781 cggtttgatc cggtcggtgt ggcggcaaaa gatctgcgtg actgcctgct gatccaactc
    57841 tcccaattcg ataaaaccac gccgtggctg gaagaggcca gactgatcat tagcgatcat
    57901 ctcgatctgt tagccaatca cgacttccgc actttaatgc gcgtcacgcg tctgaaagaa
    57961 gatgtgctga aagaagccgt caatctgatc cagtcgctcg atccgcgccc cgggcagtcg
    58021 atccagactg gcgaacctga gtatgtcatt ccagatgtgc tggtgcgtaa gcataacggt
    58081 cactggacgg tagaactcaa cagtgacagc attccgcgtc tgcaaatcaa ccagcactac
    58141 gcctcgatgt gcaataacgc gcgcaatgat ggtgacagcc agtttatccg cagcaatctg
    58201 caggatgcca aatggttgat caagagtctg gaaagccgta acgatacgct actgcgcgtg
    58261 agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc aaggcgaaga atatatgaaa
    58321 ccgatggtac tggccgatat cgcccaggct gtcgaaatgc atgaatcgac gatatctcgc
    58381 gtgaccacgc aaaaatacct gcatagtcca cgaggcattt ttgaactgaa gtatttcttt
    58441 tccagtcacg tcaataccga gggcggcggc gaagcttcct ccacggcgat tcgtgcgctg
    58501 gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt tgagcgacag caagttaacc
    58561 tctttgctgt cggaacaagg tatcatggtg gcacgccgca ctgttgcgaa gtaccgagag
    58621 tctttatcca ttccgccgtc aaaccagcgt aaacagctcg tttgacccaa ccgataagga
    58681 agacactatg cagctcaaca ttaccggaaa taacgtcgag atcaccgagg cactgcgcga
    58741 atttgttaca gccaaatttg ccaaacttga gcaatatttt gaccgaatca accaggtcta
    58801 tgttgttctg aaagtggaaa aagtcaccca cacctcagat gcaacactgc atgtaaacgg
    58861 tggtgaaatt catgccagcg cggaaggtca ggacatgtac gctgccattg atggcttaat
    58921 tgataagctg gcacgccagt tgaccaaaca taaagataaa ctgaaacaac actaattgtc
    58981 cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac aacgggccat ttgtacggtt
    59041 aatgctccga gcctgttcca ctgtttgagt gggcaggttc ttaggtgaaa ttatgacaaa
    59101 taatgataca actctacagc ttagcagtgt tcttaacagg gaatgtacgc gaagccgcgt
    59161 ccactgtcag agcaaaaaac gcgccctgga aatcatcagc gagctggcgg cgaaacaact
    59221 tagcctgccg cctcaggtgg tttttgaagc tatcctgacg cgggaaaaaa tgggcagtac
    59281 cggtatcggc aatggtattg ccattccgca tggcaaactg gaagaagata ctctgcgtgc
    59341 cgttggcgtt ttcgttcagc tcgaaacgcc tatagctttc gatgccatcg acaaccaacc
    59401 ggtggactta ctttttgccc tgctggtgcc ggcagaccaa actaaaacgc acctgcatac
    59461 tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc tgccgccgtt tacgcgcagc
    59521 ccagagcgat gaagagctgt atcaaatcat tacggatacc gaaggtactc cggatgaagc
    59581 gtagttattc ggtaatgtct cttttagacg ttgtgaggag aaacagtaca tggtactgat
    59641 gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc ctgcgtgcgc tggaagatat
    59701 gggtttttac tgcgtggata accttcccgt agtgttgtta cccgatctgg ctcgaaccct
    59761 ggccgatcgc gagatttctg ccgccgtcag cattgatgtt cgtaatatgc cggagtcacc
    59821 agaaatattc gaacaggcga tgagtaacct gcctgacgct ttctcaccgc aactcctgtt
    59881 cctggatgcc gaccgtaata ccttaattcg tcgttacagt gacacgcgcc gactgcatcc
    59941 gctttccagc aaaaacctgt cgctggaaag tgctatcgac aaagaaagcg atctgctgga
    60001 gcctctgcgt tcgcgagcgg atctgattgt cgatacctca gaaatgtccg ttcacgagct
    60061 ggcagaaatg ctgcgtaccc gtctgctggg taaacgtgaa cgcgaactga caatggtctt
    60121 tgagtctttc ggcttcaaac acggtatccc tatcgatgca gattacgtct ttgacgtgcg
    60181 cttcttgccg aacccgcact gggatccgaa actgcgtcca atgacaggtc ttgataagcc
    60241 tgtcgccgcg ttcctcgacc gccacacaga agtacacaat tttatctacc agacgcgaag
    60301 ctatcttgag ctatggttac ctatgctgga aaccaacaac cgtagctacc tgacggtcgc
    60361 cattggttgt accggcggga agcaccgttc ggtgtatatt gcagagcaac tggctgacta
    60421 cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt acgctggaaa aacgtaaacc
    60481 atgaccgtca agcaaactgt tgaaatcaca aacaagctgg gcatgcatgc ccggcctgca
    60541 atgaagctgt ttgaattaat gcagggtttt gacgctgaag tgctgttacg taatgacgaa
    60601 ggcaccgagg ctgaagccaa cagcgttatt gcgctgctga tgttggattc tgccaaagga
    60661 cggcagattg aagttgaagc gaccggtcca caggaagagg aagcactggc cgccgttatc
    60721 gccctcttta actctggttt tgatgaagat taatcttcat cactttgaca ttcaaatccc
    60781 ttcaagctcc cccggcaata agaaaagcgc gtactgaaat cgttctcaat caacgtcaat
    60841 tgtacatttc gtgcgctttt tactttcaga agaaccttaa gaaaacctta agaggcattg
    60901 tttaggtttt gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag
    60961 atggcatgat tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt
    61021 atgcgcatcc ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg
    61081 gcgataaaga gatccgtcgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag
    61141 actggagtgg aataccgcgc tatcacggta ccgtcgagac ggattgcgga accggttatg
    61201 tttatgatgt gattgctgat tttgacggca agccctccat tacgctgacc gaatttgctg
    61261 aacaatgccg ttacgaagaa gacgttgcac aactgcgcca attactgaaa cagctgaagc
    61321 gttatttgca ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc
    61381 accgcattag tgagtcagag gttatccccg tcgtctgcga caacattggt gaaagcacat
    61441 tgattccact ggcgacctgg tcaaagtggt gttgtctgcg taagcaagag cggttgtgga
    61501 aacgatttat cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg
    61561 agagcaaaac gctggccctc gcttcgcgcg aagcttaatc cagctggtgt tgttgcataa
    61621 acggctcacc gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctgcgcacgt
    61681 agcccgatgg cgcggagact ttgaaacgaa gtggattagg taatacagct gccagtaagg
    61741 cagcttccga tcgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt
    61801 cgacgccaaa cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct
    61861 ttttgctcca gaccgtttct atccccagcg ttaatccggc ttccagccct tttcgaaccc
    61921 agctacgccc atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac
    61981 cgcgaatacg gttttcattg cgctcgttgt acgccagggc tttctcaatg gaagcgacat
    62041 caaagcccca gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc
    62101 acggcgagat ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattaccat
    62161 gcagccaggc gctgacctgt cgctcgacca ttaccgcaga gaagggaaca ggcgcaacgc
    62221 taaacaacgc gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcagaagaa
    62281 aacggcgaac gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac
    62341 gcgggaaacc agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata
    62401 tgccggggtg gtaacaatct tattgtcttc atcaaccacg atatcatcga caggacacgg
    62461 cacatgctcc gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt
    62521 caaacgcagc gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc
    62581 aagcggtttt ccggcctgat gcatcgcttg tgccagcacc tttaattcac ggtcaacggt
    62641 gcattggcta ccaagactgg caaaattgct taaattcttc gccgcgccaa aaccccccgg
    62701 cacaatcaac gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg
    62761 cgttattcgt gccgcttcaa tcagcacatt gcgcgtttcc gtcatcgctt cgccagttaa
    62821 atgattgata acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct
    62881 gcgtgaaata gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc
    62941 gcatccgctc agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga
    63001 aacacattaa tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac
    63061 aaatcattta agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt
    63121 gtaactcatc ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc
    63181 gcgcaccccg gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca
    63241 cttctacgtc gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt
    63301 cccaggcagg aaggtcagga gactgaattt gctgacccag ctgttgcaga tggcgtaacc
    63361 ccactgaacc cgccgcacct ttaattttat gtccttcctc aacaatgcct tttttatcct
    63421 gcgccgtcag attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg
    63481 ctaacccgtc ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa
    63541 tatcgagcaa tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat
    63601 cctgggtatc ccagaatttc ttgatcatcg cggttagcgc cggaacagaa agcggcttac
    63661 tcagcacatc atccattcca gcattgaggt actcttgctt gtctttcagc acgttagcgg
    63721 ttaaagccac cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga
    63781 tatccagccc ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg
    63841 gtttaaacat ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt
    63901 tttccagcac agaacgcgcg acaataacgt tcagttcaat gtcttccacc agcagcacat
    63961 tcagcgccgg taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg
    64021 acggtgcgtg gatcgtcaac gtaaaggttg aacctttacc ctgttcgctg gtaaccgtaa
    64081 tatcgccgcc catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg
    64141 tggcaggttt accgccatga ctgtctttca cctggtaata catggcgaag attttatcca
    64201 gctcatcctg cggaatgccg ataccagaat cttccacttc aaaatgcagc atatcgcctt
    64261 catcgtagcg cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga
    64321 tgaggttcca caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg
    64381 gcagcgtagg ctccaggtta aagcgcaatc ctttttgttg cgcctgcaag gcggagagat
    64441 tttccagatc ggcaaggaag ctggtgaaat caaccggctg gttatcaagc tggaccttgc
    64501 gccgttccat cttatccatg tcgataatat cgttgaagat attccccagc gtgacggccg
    64561 aaacatggat agttttgaga tatttttcct gctcggcggt gagttcggta tccagtagga
    64621 tgcggctcag gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga
    64681 taaacgtcgt tttgtcgcgg ctggcgcgtt caagcgcatc ctgataccgc ttacgctcgg
    64741 taatgtcgcg accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca
    64801 ctttacggat ttcaaagcag gctttgcgtc cgtcgggata atccagccac tgctcatagg
    64861 tcagtgacac attatgacgg aacacttttt catcggtttc aatgactttc gcagcggctt
    64921 ccggtgagta aacatcagca ggtttcagat gaaccagttg tttttcgctt tttccggtca
    64981 gcagctccat cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca
    65041 ggtcgggtga agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt
    65101 gtgtctcttc gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg
    65161 cttccgcttt ttcacgaacg gcaatttcct gatttagctg ggcaatatta tcttttaact
    65221 gaacgttgag gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca
    65281 gacgttgtcg tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg
    65341 gcgtaatcag caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat
    65401 gcagcaccat ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca
    65461 gcaacattga gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca
    65521 gcagacgaat ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa
    65581 ttctgacagt tacgttataa attgtgtgag aaatgcggaa ttcccctcaa tgacctactc
    65641 cgtaggaggt atccacggac gtcccagcgc cgatccctgt acgccatgtt cattcagata
    65701 gcgatccagc tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt
    65761 cggacgacgg gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat
    65821 catttctccg gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc
    65881 ccaggctttc gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc
    65941 cacgccggtt tcaaccacgc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg
    66001 caggccgaca attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt
    66061 acgctgataa caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc
    66121 ggtttgtagc cccagctcca gccacacttc gtagccctgg tccttatatt cgcaaagcag
    66181 atccagcacc gcatccggca cgcagtccgg gcgagtacca acacacagac cgacaatatt
    66241 ggcctggctc accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct
    66301 ggtatacgcc tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg
    66361 cgccagttgc tcggcaatgg aacgatgttg ctgcgcttca tcagcaaacg aggcaacatt
    66421 acagaatgtg cagccgccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc
    66481 atgcagcgtc agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat
    66541 attgactaat ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc
    66601 ctgccatttt tagctcattt cggcgatgac ctggatcaat cgtcccggcc tgcctttacc
    66661 aactgcataa tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc
    66721 ttatcttgtg tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa
    66781 acgtcattac caattaaggc agtataaaat gctggttttc tcgtcagttc aaggcaggat
    66841 aagggttaac acgcctttat gacagtcagg aattggctgt ttctctaacg acgctccttt
    66901 tatccttaat tagaaaatcc attttaattt cagtcaatta ataaagaatt ttgcgctaaa
    66961 gcacatttct gtaccaataa gcttgccatt tgacctgtat cagctttccc gataagttgg
    67021 aaatccgctg gaagctttct ggatgagcag cctgctcatc atatttatgc agtaattgag
    67081 atcccctctt caccgtatta atcgatgcga aaaggacaac aagggggcga atgcgaggcg
    67141 cgcgtatgac acgcaaaccc cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg
    67201 tagtggggtt cccgcagagc ctgggggagg ttcacgatat gttgtacgat aaatcccttg
    67261 agagggataa ctgtggtttc ggcctgatcg cccacataga aggcgaacct agccacaagg
    67321 tagtgcgtac tgcaatacac gcactggccc gcatgcagca ccgtggcgcg attctcgccg
    67381 atggtaaaac cggtgacggt tgcggcttgc tgttacaaaa accggatcgc ttttttcgca
    67441 tcgttgcgca ggagcgcggc tggcgtttag caaaaaacta cgctgtcggg atgctcttcc
    67501 tgaataaaga tcctgaactc gctgctgccg cacgccgcat cgttgaagaa gagctgcaac
    67561 gcgaaacgtt gtcgattgtg ggctggcgtg atgtccccac taacgaaggc gtgctgggtg
    67621 aaatcgccct ctcctctctg ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct
    67681 ggcgtccgcg tgatatggaa cgccgtctgt ttatcgcccg ccgccgcatc gaaaagcgtc
    67741 tcgaagccga caaagacttc tacgtctgta gcctgtcgaa tctggtgaac atctataaag
    67801 gtctgtgtat gccggcggat ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg
    67861 aatcggccat ttgcctgttc caccagcgct tctccactaa caccgtaccg cgctggccgc
    67921 tggcgcaacc gttccgctac ctggcgcata acggtgaaat caacaccatc accggtaacc
    67981 gccaatgggc tcgtgcgcgt acttataaat tccagacacc gcttatccct gacctgcacg
    68041 acgcagcgcc attcgtcaac gaaaccggtt ctgactccag ctcgatggat aacatgctgg
    68101 aactcctgct ggcgggtggg atggatatca tccgcgctat gcgtctgtta gtgccgcccg
    68161 cctggcagaa caacccggat atggacccgg agctgcgtgc cttcttcgac tttaactcca
    68221 tgcatatgga gccgtgggat ggcccggcgg gcatcgtgat gtccgatggt cgttttgccg
    68281 cctgtaacct cgaccgtaac ggtctgcgtc cggcacgcta cgttatcacc aaagataagc
    68341 tcatcacctg tgcctctgaa gtcggtatct gggattatca gcctgacgaa gtggtcgaaa
    68401 aaggccgcgt cgggccaggc gaactgatgg ttatcgacac ccgcagtggg cgtattctgc
    68461 actcggcaga aaccgatgac gatctgaaaa gccgccatcc atataaagag tggatggaga
    68521 aaaacgtccg ccgactggta ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg
    68581 aactggacga cgacacactt gccagctacc agaaacagtt taactacagc gcggaagagc
    68641 tggactccgt aattcgcgta ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg
    68701 acgacacccc attcgccgtg ctctccagtc agccgcgcat tatttacgac tacttccgcc
    68761 agcagtttgc ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg catgttatgt
    68821 cgctcgccac cagtatcggt cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc
    68881 accgtttaag ctttaaatcg ccgattctgc tctactccga tttcaaacag ctcacgacga
    68941 tgaaagagga gcactaccgc gcagatacgc tggatatcac ctttgacgtc accaaaacca
    69001 cgctcgaagc gacggtcaaa gagctgtgcg acaaagccga aaaaatggta cgtagcggca
    69061 ccgtgctgct ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg gttccggctc
    69121 caatggcggt tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca
    69181 acatcatcgt cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg ttgctgggct
    69241 ttggtgcgac ggctatttat ccgtaccttg cctatgaaac gctgggccgt ctggtagaca
    69301 cccacgcgat tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca
    69361 aaggcttgta caaaatcatg tccaaaatgg gcatctccac catcgcctct taccgctgct
    69421 cgaaactgtt tgaagcggtc ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg
    69481 cggtcagtcg cattggtggg gcaagctttg aagacttcca gcaggatctg ctgaatctgt
    69541 cgaaacgtgc ctggctggcg cgcaagccca tcagccaggg cggcctgctg aaatacgtcc
    69601 acggcggcga ataccacgcc tacaacccgg acgtggtgcg cacgctgcaa caggcggtac
    69661 agagcggcga gtatagcgac tatcaggaat acgcgaagct ggttaatgag cgtccggcaa
    69721 ccacgctgcg cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac attgccgatg
    69781 ttgaaccagc aagcgaactg tttaaacgct tcgataccgc ggcgatgtct atcggcgcgt
    69841 taagcccgga agctcacgag gcgctggcgg aagcgatgaa cagcatcggc ggtaactcta
    69901 actccggtga aggtggtgaa gacccggcgc gctacggcac caacaaagtg tcgcgcatca
    69961 agcaggtggc gtccggtcgc ttcggtgtta ctccggcgta cctggtcaat gctgacgtca
    70021 ttcagattaa agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg ccgggcgaca
    70081 aagtcactcc ttacattgcc aaactgcgct attcggtgcc gggcgtgacg ctgatctccc
    70141 cgccgccgca ccacgatatc tactctatcg aggacttagc gcagctcatt ttcgacctca
    70201 agcaggttaa cccgaaagcg atgatctccg tgaagctggt ttccgaaccg ggcgttggaa
    70261 ccatcgcgac cggcgtggca aaagcttatg ccgatttaat caccatcgca ggctatgacg
    70321 gtggcaccgg cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg tgggagctgg
    70381 ggcttgttga aacccagcag gcgctggttg ccaacggctt gcgtcataaa atccgtttgc
    70441 aggtcgatgg cggcctgaaa acgggtgtcg atatcatcaa agcggcgatt ctcggcgcag
    70501 aaagcttcgg cttcggcact ggcccaatgg tcgcgctcgg ctgtaaatat ctgcgtattt
    70561 gccacctgaa caactgcgca acgggtgtgg caactcagga tgacaaactg cgtaagaatc
    70621 actatcacgg cctgccgttc aaggtgacga attactttga gtttatcgcc cgtgaaaccc
    70681 gcgagctgat ggcacagctt ggcgtaacac gtctggtgga tctgattggt cgcaccgacc
    70741 tgctgaaaga gctggacggt ttcaccgcca aacagcagaa actggcgctg tcgaagctgc
    70801 tggagaccgc cgagccgcat gcaggtaagg cgctctactg taccgaaaac aatccgccgt
    70861 ttgataacgg actgctgaac gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgaac
    70921 gccagagcaa aactttctgg ttcgatatcc gcaataccga ccgttctgtc ggcgcgtcgc
    70981 tttcaggcta tatcgcccag acgcacggcg atcagggtct ggctgccgat ccgatcaaag
    71041 cgtacttcaa cggcaccgca ggccagagct tcggtgtgtg gaacgcgggc ggcgtggagc
    71101 tgtatctgac gggcgatgcc aacgactatg tcggtaaagg catggcgggc ggcttaatcg
    71161 ccattcgtcc tccggttggc tcagccttcc gcagccatga agcaagcatt atcggcaaca
    71221 cctgcctgta tggcgcgacg ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt
    71281 tcggcgtgcg taactccggt gcaatcaccg tggtagaagg cattggcgac aacggctgtg
    71341 aatatatgac gggcggtatc gtctgtattc tgggtaaaac cggcgttaac ttcggtgcgg
    71401 gtatgaccgg cggcttcgct tacgttctcg atgaaagcgg cgatttccgc aaacgcgtta
    71461 acccggaact ggtggaagta ttaagcgttg acgatttggc gatccatgaa gagcatctac
    71521 gtggtcttat caccgagcat gtgcagcata ccggttccca gcgtggtgaa gagattctgg
    71581 cgaactggtc aaccttcgcc actaaatttg cgctggttaa accgaagtcc agtgatgtaa
    71641 aagcactgct gggtcaccgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag
    71701 gggtagcaac aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc
    71761 ctaagaaacc gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg
    71821 aaggccaggc caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt
    71881 ggaaatgccc ggtacacaac tacatcccga actggctgaa gctcgccaac gaggggcgta
    71941 tttttgaagc ggcagaactg tcgcaccaga ccaacaccct gccggaagtt tgcggacgtg
    72001 tctgcccgca agaccgtctg tgcgaaggtt cctgcactct gaacgatgag tttggcgctg
    72061 tgaccatcgg caacattgag cgttatatca atgataaagc gttcgaaatg ggctggcgtc
    72121 cggatatgtc cggcgtgaaa cagaccggta aaaaagtggc gattatcggc gcaggtccgg
    72181 caggtctggc gtgtgcagat gtcctgacgc gcaacggcgt acaagcggtc gtcttcgacc
    72241 gtcacccgga aataggcggc ttgctgacct tcggtattcc ggctttcaaa ctggaaaaag
    72301 aggtaatgac gcgccgccgt gaaatcttca ccggcatggg tattgaattc aaactcaata
    72361 ccgaagtggg ccgcgacgtg cagctggacg atctgctgag tgattacgat gccgtgttcc
    72421 ttggcgtcgg gacttatcag tcaatgcgtg gcgggctgga aaacgaagac gccgatggcg
    72481 tgtacgcagc gctgccgttc cttatcgcca acaccaaaca gttaatgggc tttggcgaaa
    72541 ctcgtgaaga accgttcgtc agcatggaag gcaaacgcgt ggtagtactt ggcggtggcg
    72601 ataccgcgat ggactgcgtg cgcacgtctg tacgccaggg ggcaaagcac gttacctgtg
    72661 cctatcgtcg tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg
    72721 aagaaggtgt agagttcaaa ttcaatgtcc agccactggg aattgaagtg aacggtaacg
    72781 gcaaagtcag cggcgtaaaa atggtgcgta ctgaaatggg cgaaccggat gccaaaggcc
    72841 gtcgccgcgc ggagatcgtg gcaggttccg aacatattgt ttcggcagat gcggtgatca
    72901 tggcgtttgg tttccgtcca cacagcatgg aatggctggc aaaacacagc gtcgagctgg
    72961 attcgcaagg ccgcatcatc gccccggaag gcagcgacaa cgctttccag accagcaacc
    73021 cgaaaatctt tgctggcggc gatatcgtcc gtggttccga tctggtcgtt accgctattg
    73081 ccgaaggtcg taaggcggca gacggcatta tgaactggct ggaagtttaa gctctgtccc
    73141 agcgccgcct cctcagaaaa gagtcggcgc tgaaattctc ctttcgttct ggaaagcgcc
    73201 tcgcataatc tgaagaaaac caatgtaata cgcacatctg gattgttacc ggcagatgcc
    73261 cggcgtatag ctgattccat tattcaacac tgcgccatat aaagggaaag ataatggaat
    73321 cgctctcaga gagaacctca acaggctacc agcaaatcca cgacggtatt attcacctgg
    73381 tcgatagcgc ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact
    73441 gggaaattgg ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg
    73501 gtgcgcagct aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct
    73561 ctgcaaaaaa cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc
    73621 gccagacagt gtctggcgaa ttaacaccat tgggaattcc gcagacacca tctgcagaat
    73681 tcccatcggc aaagatttgg cagacgctgt ctgccaaatc tttccccctg ccctggtcca
    73741 cctacgtccg tttactttcc gttaaaaacg ctgacgcccg cagcttttat gaaaaagaga
    73801 cgctccgctg tggctggtct gttcgccagc tagaacggca aattgcgacc caattttatg
    73861 agcgaacact gctgtcacat gacaaatcag ccatgctgca acaacacgct cctgccgaga
    73921 cgcatattct tccgcaacag gcgatacgcg atccctttgt gctcgaattt ctggaattga
    73981 aagatgaata ctcagaatcc gattttgagg aggcgctgat caaccacctg atggatttca
    74041 tgctggaacc tggggatgat tttgcctttg ttggtcggca gcgaaggtta cgcattgatg
    74101 acaactggtt tcgtgtcgat ctgctgtttt tccaccgccg tttacgctgc ctgctaatcg
    74161 tcgatctaaa agtgggcaaa ttcagctata gcgatgccgg acagatgaat atgtatctca
    74221 actacgccaa agagcactgg acgctaccgg atgaaaatcc gcccatcggt ctggttctct
    74281 gtgcagagaa aggagtcgga gaagcgcatt atgcactggc tggtttgcct aacaccgttc
    74341 tggcgagtga atataagatg caactacctg atgaaaaacg actcgcggat gaactcgttc
    74401 gaacacaggc ggtgctagaa gaaggctata gactccgtta atttcagctg tttcgaattc
    74461 tgcagacact gtctgcagaa tctttaaaac gaactaagtt ttaagccatt aaatcagcct
    74521 taaccttcac cacaaccttt ttaatctcac caggttcgcc gacaacgcat cctggtttat
    74581 gcggttctcc cggcataaac acggcgaaca ttcccggttt taagttgatg gtttgctcgt
    74641 tttcaatggc actgcaaagc tggtaatcat cttcatggtg gaactcttca cactggcgcg
    74701 cagtgcctgc cgtgccaaac agaatccgtt cctcaccttt taataacagc tggatatcaa
    74761 tgtattgctc gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga
    74821 cattcataaa aatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc
    74881 tggcagctaa tgccagcgtt aacgcgtcct gtaacacagg atgtaaccca gcagacggta
    74941 atgactgtac ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag
    75001 taaacctgca tcatggcggt agtgcgccgc cagtaaatca acatgaaatg ccgctggctc
    75061 ctgcgccaga tacgtttcca ccagcgccag atacccttct gccagaccaa cgctgccacc
    75121 gaccaccacg cactgacaat cagttgtggc tttaatatca gcgatcagcc ttgccagcac
    75181 atgtgcggag cggtgaatca gctgctgcgc ctgctcgtcg ccctgcccgg cgcgcatgaa
    75241 aatagttttc gcatccgcgc cagccaactc cccctgcgct gccgctgcaa taccgcgacc
    75301 agaagcaatc gcttccacgc aacctgtgcg tccacagccg cagactgggc cgtgtggatc
    75361 ggctagcgta tgcccgatgt gccccgccag accgccaggg ccggtaagca gtttgccgcc
    75421 gctcactaca ccgccgccga cgccagtgga aacggtgata aacaccatct cggttacatc
    75481 gccttccagc gcctgatact ctgcccatgc tgcggcctgc gcgtcgttaa tggcaatggt
    75541 cggcaaattg gtaagttgtt ccagcgtttt gactaacgga aagtgtaaca atccaccaag
    75601 attatgcgga ttcagcgcca gcaagctgcc gtcgcgaata atcccggttg aagcgatggc
    75661 aacccgctgc gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcagtgc
    75721 ctgcggagtc tggctggcag gcgtaggaag ttctcggcga tcgcggatct gcccgtcagc
    75781 gccaatcagc gcggcggcaa gtttagtacc gccgatatca atcgccagcg tggtcatagc
    75841 accgcctttt tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca
    75901 gaaccgaccg tcaccgccca cgcgccgtgg cgcatcgcat cagccgcctg agcaggcgtg
    75961 ttgtaacgcc cttcggcaat cactcgacat ccggcgtcac tcaacctttt caccagcgtc
    76021 agatccggct cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt
    76081 tcggctccca gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc
    76141 agtaaaccgt gatgatgaat acgtgccagc agcgtttcaa caggcaccgg acgcggtcgg
    76201 tcagtgccgt caatggcgat aatgtcagcg cccgcctgcg ccagcgcatc aacatcttca
    76261 atataggccg tgatgcgtac cggagaatcc tccagatcgc gtttcacaat cccgataatc
    76321 ggcacgctca ccaccgcacg cgtggcttgc agatttgcca caccttcgat gcgaatggca
    76381 accgcgcccg cctgctctgc cgctaatgcc atggcagcga caatgtcggg tttatcgagc
    76441 gggctgtccg gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga
    76501 tccagttgtg caagtaacga catacatctt cccttagcga aaggcccggt acagagaccg
    76561 ggcaacagga ttaacttttg gttttgacta aatcgttttt ggcgcggcca aatggcacgg
    76621 caccgctaaa tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc
    76681 aacgctgaac acgggaaggc atatccagac caatcagcag gatcaccaca aatgtcagac
    76741 tgaacgagag cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga
    76801 tgattggggc cagtgcgcca cccaatgcgc caacgttata ggtaaagccc aggcccgctg
    76861 cacgctggtc ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt
    76921 gtccaagcat ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa
    76981 tcgcaaatac cggaataatc agcagctgcg aggccaacag actacaaacg tacgctttgc
    77041 gggttcccag ccagtcaccg aggaagccac cgacgcagca ccccaccgcc gcgccaaagc
    77101 cactaaagaa cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat
    77161 acgttggcag cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca
    77221 tcagcattac gccagttggc cagcgtttgc ccgtactctg caccataaag ctgataaaga
    77281 ttgcggcgca taacagccca agaacagcga cgatcgcggc attttgcagg ttaccggcga
    77341 agcagaacca cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt
    77401 caccacggta gagaatatcc accattgtgc gtactggtgc tttacctgcg tgtttctctt
    77461 tccagtcttc cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa
    77521 tgccgataaa gaacagcgca cgccagcccc agaccggaac caccaggctg tagacctgag
    77581 cggcaacgac ggcccctaca gagaagcctg aaatcagaaa gccactggct ttgttacgca
    77641 gatgttttgg ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc
    77701 ccatgccgat gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg
    77761 ccagcgtccc ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc
    77821 ggtcacccat agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga
    77881 tcagacttgc cgcctgcacc gtcgtcagcc cgaattcacc ttgtacttcg gtgagtacca
    77941 gggcgattaa aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg
    78001 cccgccattg tgcacgattg agatggcgat accacgggat attctgggtt gtagtactca
    78061 ttgtgagtct cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca
    78121 gatgcgttta tttcccctca cccggtaggg gcgagcgagg ggaaacaact cacccgcgct
    78181 cttgcatcaa ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggac
    78241 caaacggttt gcggcacagc ggcacagaca cgacatccat ataatgcaga acagttttca
    78301 ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac tttattgcac tcagtttgca
    78361 gtttctgcgc ggtctggata tcgccttctt tcagcgcctt cacgatcccc tgatatcgcc
    78421 agcccatgat attgtaagta ctgccgatac cgccgtctgc gcccgccagc aagccggaag
    78481 cgaagatttc gtcgtaaccg ttatagagca ccagatcagg atgttcacga cggatctgtt
    78541 ccatctgata gagatcgcca gaggtctgtt tcagcgcgcc gacaccaggc agcgtaacca
    78601 gggtgttgat ctgatccagg gtcagtttta ccccgctcag ggctggaatg ttgtacacca
    78661 ccatcggcaa accatccgcc gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa
    78721 agctgaaagg atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag
    78781 ccgatgccgc aagttgttgg ctttcggcgg tgctaacgca accgacgtgg gcgatgagtt
    78841 taatcttacc tttcgcctct tcggcgacga tttccagtac ctgttcacgc tcggaaaggc
    78901 tttgtacaaa ggcttcgccg gtcgaaccac ccacgtataa accgtcaatg ccctgctgaa
    78961 tattgaactg aaccaggcga cgcagactcg ctttatccag tgcttgttgt tggtcaaaag
    79021 gagtcaggag tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga
    79081 tgcttgtctg ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc
    79141 ccatacagaa agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg
    79201 gtggtctgac cgaaggcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgca
    79261 cgatcggctt catcgggatc gtggcggcgg atcgcgtcaa cgatcgcaat atgctgttga
    79321 tagctaacgt tgttatgctc gtgcagagcc tgatcagcca cggttggacg tgcggcgatc
    79381 agccagtcga gcagggcaac gtggatcgcc atgaagattg ggttacctgg gatctccgcc
    79441 agcacgcggt ggaaatcaac gtctgagcga atgaatgccg cgttgttatc cagcgactga
    79501 ctgttgattt ccagtgcttt tgccagcaaa tcgatttgct catcggtggc gtgttcagcc
    79561 gcatagcgca ccaggctgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca
    79621 ccaggatgag aaaggaaatc tttcgccatg ccggaaagct cgccgatgat ggtgtccgca
    79681 gaaggacgtg agacgcgggc acgttcgccg ttgtttattt gcaccagacc tttgcgtttt
    79741 aacgctgcca gcgcttcacg caccgaagga cgcccgacgt taaagaacgc catcagttcg
    79801 cgttcagacg gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc
    79861 tcttcttcca ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga
    79921 ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg
    79981 taaggtcagt gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa
    80041 aatggcaggg ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat
    80101 tgcgaaaaat taatgtaacg cagataaaaa catcccgatt gaattattta taagactatt
    80161 cacgagcatt atgaatatta tgaatgtgtt cttacaaaat aatcataagc gcatattttt
    80221 aatgaaaaat cacctcacct acaattaaaa acacgacatc agcaccataa atagacttgc
    80281 aaaaaatata acatcattgt tttcaatctg ccatttatgg gattgaccgt tttcttttga
    80341 cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct
    80401 atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt atcttcctga
    80461 tgatgtgcgg tgtctggtta gggttcggtg gtgtactcga tcccaccaaa agcagcggct
    80521 acttgatcgt cgatatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat
    80581 tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc ataggggcca
    80641 gtcgcgcgat ggtgagcttg ttaagccgcc cgttaaaact cattcgctcg ccgtatatta
    80701 ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc agcgcctccg
    80761 gcctgggtat gttgctgatg gtcaccttat ttccgacact ggtgagtctg ggagtaagcc
    80821 gtctctctgc ggtggcggtt atcgcaacca cgatgtccat tgagtggggg attctggaaa
    80881 cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat tgccacttac ttcttccact
    80941 accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac tttttcgtgc
    81001 aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag caaaaaactc
    81061 tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta atcctgatgc
    81121 tcggctcgct attccttgcc cacatcgggc tgatgcagtc agaactgcat ctggtggtgg
    81181 tgatgttact gagtttgacg gtgacgatgt ttgttgagtt cttccgcaag cataaattac
    81241 gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgccaacg
    81301 tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg
    81361 tcgatgcggt tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta
    81421 ttatggcgct ggtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta
    81481 tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa
    81541 tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg attactgcgg
    81601 tggtggtcgt tacgtcagga attgcaggcg tttcgccttt tgcggtggtg aagcggacag
    81661 cgatccccat ggcagtcggt ttcgtggtga atatgattgc cacaatcacg ctattttatt
    81721 aagtcattaa aagacaaaac aggccgccag ggcctgtttt gtattacttc acaacgcgta
    81781 atgccggtcg accaccgcgt ggtggctgcg gaggttcatc gtcaggatga gtgtcatcat
    81841 cgtgatctgg cttatcgcca tcaataaccg acataacggt ttcgttgtct gccgatgcct
    81901 cttcatcatt catgatgctg gtatcttcat cgtaggcagc ttcaggctca aacatcgtgc
    81961 ctgcgccatt ttcacgggcg tagatagcca gtacggcagc cagcggcaca gaaacctgac
    82021 gcggaatgcc accaaagcgc gcgttaaagc gcacctcatc attcgccagt tccagattgc
    82081 cgacagcacg cggcgcaatg ttgagtacga tttgcccgtc acgcgcatat tccataggaa
    82141 cctgcacgcc agggagcgtc acatccacca ccaggtgcgg cgtgagctgg ttatccagca
    82201 accactcata gaatgcacgc agcagatagg gacgacgtgg tgttagctgt gacaaatcca
    82261 tacagattaa ctccggccca gacgcatttc acgttctgct tcagttaaag aagcaaggaa
    82321 agagtcacgc tcaaagacgc gggtcatata gcctttcagc tctttcgcac ccgggccgct
    82381 gaactcgata cccagttgcg gcagacgcca cagcagcgga gcaaggtagc aatcgaccag
    82441 gctgaactca tcgctcagga agtacggctt ctgaccgaag accggcgcaa tcgccagcag
    82501 ttcttcgcgc agttgcttac gtgcggcatc tgcttcagaa gctgaaccgt tgatgatggt
    82561 gttcatcagc gtgtaccagt ctttttcgat gcgatgcatg tacagacggc tttcaccgcg
    82621 agctaccggg taaacaggca tcagtggcgg atgcgggaaa cgctcatcca gatattccat
    82681 aatgatgcga gattcccaca gggtcagctc acgatccacc agggtcggaa cgctctgatt
    82741 cgggttgagg tcaatcagat cctgaggcgg attgtccttt tccacgtgtt cgatctcgaa
    82801 acttacacct ttctcagcca gcacaatgcg gacctgatgg ctatagatgt cagtaggacc
    82861 ggaaaacagc gtcattaccg aacgtttgtt ggcagcgaca gccatgaaaa cctccaggta
    82921 tagtcagaat ttttactgct accagccacc aggtggccag tcagaagttg tgttacccag
    82981 taaggaacga ctctctttgt tcgaaaatca aacaaaaaat gagcaatacc cgacatttgg
    83041 gcagaaaatt ggatgatagt ttaccagatt ttgcgacctt tgtggtgagt cgatgccgga
    83101 aatggggaaa aagagatgcg ctttagtctg aaatagttga cttagtccct tattggcgat
    83161 gtggtttttg ttttgcctgt ctgtcagacg gcagcaaaaa gcaactttcc attttttacg
    83221 ctgattcaga atttaaccat aaaaaaaccc gccgaagcgg gttttttcgc aaattgtttt
    83281 ctgccggagc agaagccaat taacgtttgg agaactgcgg acgacgacgt gctttacgca
    83341 gaccgacttt cttacgttca acctgacgag cgtcacgagt aacgaagcca gctttacgca
    83401 gttcagaacg cagggactcg tcgtattcca tcagagcgcg ggtgataccg tgacggatcg
    83461 caccagcctg accagagata ccaccacctt taacggtgat gtacaggtcc agtttctcaa
    83521 ccatgtcgac cagttccagc ggctgacgaa ctaccatgcg ggcagtttca cgaccgaagt
    83581 actgttccag agaacgttgg ttgattacga ttttaccgtt gcccggtttg atgaaaacgc
    83641 gagctgcgga acttttgcgg cgaccagtgc cgtagtattg attttcagcc attgcctata
    83701 atcccgatta gatgtcaaga acttgcggtt gctgtgccgc gtggttgtgc tcgttacccg
    83761 cgtaaacttt cagtttacgg aacatagcac gacccagcgg gccttttggc aacatgcctt
    83821 taaccgcgat ttcaatcaca cgctcaggac ggcgagcaat catctcttca aaggtcgctt
    83881 gtttgatacc accgatgtgg ccggtgtggt gatagtacac tttgtcagta cgcttgttgc
    83941 cggttacagc aactttgtca gcgttcagaa cgatgatgta atcaccggta tctacgtgcg
    84001 gagtgtattc cgctttgtgc ttaccgcgca ggcgacgagc cagttcagta gccagacggc
    84061 ccagagtttt accggtcgcg tcaacaacat accagtcgcg ttttacggtt tctggtttag
    84121 ctgtaaaagt tttcattaaa agcttaccca ataaatagtt acacgttggt gaacacccaa
    84181 acgtcttcaa ttgttgaggt tcacacgaca aagtccggca aacctacccc ttcgaatagc
    84241 ctatgccagc acacaaaaag ttttgggaaa aaaactttct tgtaacgtgg ggtcgcagga
    84301 ttatagagaa gtcggggtca aagatcgacc cctttttgtg atttgtggca ggttttaacc
    84361 cgctaaatgc tcgcgcttca gatactcttc gctttgcatc tcttgcagac gtgacaggca
    84421 acgctggaac tcaaatttaa gtcgctcgcc ctgataaatt tcatacagcg gcacttctgc
    84481 actcaccact aatttgacat ggcgctcgta aaactcatcc accagcgcaa taaagcgccg
    84541 cgcttcactc tccatcaacc gcgtcataac tggtacatca aacaacatga ccgtatgaaa
    84601 gaggcgtgag agcgcaatat agtcatgctg actgcgggcg tcgacgcaca gcgtagtaaa
    84661 agagaccgcc agcgtctggt tctcgacgcc cattgtcgct aatggccgat ggttgatttc
    84721 taacgtcggt gaattttctc gtttcccccc cgccagcgcc aaccatagtt tatccatttg
    84781 cgcccgggtt tcatcgtgga gtggcgaaag ccacagatgc gcctgagtga gtgtacgcag
    84841 tcgataatca acaccagcgt ccacgttcat tacatcacaa tgctgtttaa tggcatcgat
    84901 tgcaggcaga aaacgcgcac gttgcaggcc atttcgataa agttcatccg gcggaatatt
    84961 tgacgtcgct accagggtaa taccgcgggc gaacagggct ttcatcagac cgccaagtag
    85021 catggcgtcg gtaatatcag aaacaaaaaa ttcgtcaaaa cagagcacgt cagtttcggc
    85081 tttaaagcga tcggcaataa tttccagcgg atcggtctgc ccctgtaagg cagttagctc
    85141 ttcgtgcacc cgcagcataa aacggtgaaa gtgcaggcgc tgtttccgct ctcccggcag
    85201 gctttgatag aaaaggtcca tcagccaggt tttcccgcgt cctacaccgc cccacatata
    85261 taagccacgc actggcgtat gctttgtgtc ttcgcgttta ccccacagct taccgacccg
    85321 cgccattagc ccactcgtcc tgggggctgg tggcgtgcta ttgatgagtt cctgataaat
    85381 aatttccagg cggctgacgg cctctttttg aacgtcgtcg ggttgatggc tgccttcatt
    85441 gagcgccttc aggtattgcg atgttggggt aacgctttgc atgatcttat tgttattcct
    85501 tgaataatcg gtgcgccgtt gttcacggtt gacgaaaaaa aggccgttct acactacgcg
    85561 atatgcagtc gggattccac ttctgtggaa ttaacggtta tagtggcata atcagccgca
    85621 ggcatggagc ctgaagccaa caccctacgg aaacaaaaga caacgggaga tgttcatgac
    85681 ctgggaatat gcgctaattg ggttagtcgt cggcatcatt attggtgctg tggccatgcg
    85741 ttttggtaat cgtaaactac gccagcaaca ggcgttgcag tacgaactgg aaaagaataa
    85801 agctgaactg gacgagtatc gcgaagagct ggttagccac tttgcccgca gcgcggaatt
    85861 actggatacc atggcgcacg actatcgcca gctgtatcag cacatggcga aaagctccag
    85921 cagcctgctg ccggaactgt ctgctgaagc aaacccgttc cgtaatcgtc tggcagagtc
    85981 tgaagccagc aacgatcagg caccggtgca gatgcctcgc gactattctg aaggcgcatc
    86041 cggcctgctg cgtactggcg cgaagcgcga ctaatttatt tttcgggcgc agccattgcg
    86101 ccctcctctt ctctccctcc ccgactatca tttaatctgg tgtctcattg ttagccgtct
    86161 gaaaattcaa taacatcaaa ctgttttgaa tctcttttct tatcattcag gtacgagagc
    86221 aggaataatg aaaaaacaaa cccagctgtt gagtgcatta gcgttaagtg tcgggttaac
    86281 tctctcggcg tcatttcagg ccgtcgcgtc gattccaggc caggttgccg atcaggcccc
    86341 cctccccagt ctggctccaa tgctggaaaa agtgcttccg gcagtggtga gcgtacgggt
    86401 ggaaggaacg gccagtcagg gacagaaaat cccggaagaa ttcaaaaagt tttttggtga
    86461 tgatttaccg gatcaacctg cacaaccctt cgaaggttta ggctccggtg tcatcatcaa
    86521 cgccagtaaa ggctatgtgc tgaccaacaa ccatgtgatt aatcaggcac agaaaatcag
    86581 tattcagctc aatgatgggc gcgagtttga tgcaaaactg attggtagcg atgaccagag
    86641 cgatatcgcc ctgttgcaaa ttcaaaaccc gagcaaatta acgcaaatcg ctattgccga
    86701 ctccgataaa ttgcgcgtcg gtgattttgc cgtggcggtc ggtaacccat ttggccttgg
    86761 gcaaaccgcc acctctggca ttgtttccgc attaggacgc agcgggttga atcttgaagg
    86821 tctggaaaac tttatccaga cagatgcttc cattaaccgc ggtaactccg gcggtgcact
    86881 gttaaacctt aacggtgagt taattggcat caacactgca atccttgcgc ctggcggcgg
    86941 gagcgtcgga attggatttg ccatccccag taatatggcg cgaacactgg cgcagcagct
    87001 tatcgacttt ggtgaaatca aacgcggttt gttaggcatc aaaggcaccg agatgagtgc
    87061 cgatatcgcc aaagccttca accttgacgt gcagcgtggc gcgtttgtca gcgaagtgtt
    87121 gccaggttct ggctcggcga aagcgggcgt caaagcgggc gatattatta ccagcctcaa
    87181 cggcaaaccg ctgaatagct ttgctgagtt gcgctctcgt atcgcgacca ccgagccggg
    87241 cacgaaagtg aagcttggcc tgctgcgtaa cggcaaacca ctggaagtag aagtgacgct
    87301 cgataccagc acctcttcgt cggccagcgc tgaaatgatc acgccagcgc tggaaggtgc
    87361 aacgttgagc gatggtcagc taaaagatgg cggcaaaggt attaaaatcg atgaagttgt
    87421 caaaggaagc ccagctgctc aggctggctt gcaaaaagac gatgtgatca ttggcgtcaa
    87481 ccgcgatcgg gtgaactcga ttgctgaaat gcgtaaagtg ctggcggcaa aaccggccat
    87541 catcgccctg caaattgtac gcggcaatga aagcatctat ctgctgatgc gttaatgtcg
    87601 taaaccgggc atcaggctga cgtgtgatgt ccggttaact cgtggtatgc tgctgccgtt
    87661 ccctttttta atgacgcctc catcatgttt gtgaagctct tacgttccgt tgcgattgga
    87721 ttaattgtcg gcgctattct gctggttgcc atgccttcgc tgcgcagcct taacccgctt
    87781 tccactccgc aatttgacag taccgatgag acgcctgcca gttataatct ggcggttcgc
    87841 cgcgccgcgc cagcggtggt taacgtttac aaccgtggtt tgaacaccaa ctctcacaac
    87901 cagcttgaga tccgcaccct gggatccggt gtaatcatgg atcaacgcgg ttatatcatc
    87961 accaataaac acgtcatcaa cgacgccgat cagatcatcg tcgccttaca ggatggacgt
    88021 gtatttgaag cattgctggt gggatctgac tctctaaccg atctggcggt acttaaaatt
    88081 aatgccactg gcggtttacc taccattccg atcaatgcac gtcgcgtacc gcacattggc
    88141 gacgtggtac tggcgatcgg taacccgtac aacctcgggc agaccattac ccaggggatt
    88201 attagtgcca cgggtcgaat cggtctgaac ccgaccgggc ggcaaaactt cctacaaacc
    88261 gatgcttcca ttaaccacgg taactctggc ggcgcgctgg tgaactcgct gggagaactg
    88321 atgggcatta acacgctgtc gtttgataag agtaacgatg gcgaaacgcc ggaaggtatc
    88381 ggctttgcga ttcctttcca gttagcaacc aaaattatgg ataagctgat ccgcgatggt
    88441 cgcgtgatcc gcggctacat tggtatcggc ggacgcgaga tcgcaccact gcacgcgcag
    88501 ggcggtggta tagatcaact gcaagggatc gtggttaatg aagtgtcacc tgacggcccg
    88561 gcggcgaatg cgggtattca ggtcaacgat ctgattattt cggtggataa caaaccggcc
    88621 atctctgctc tggagacgat ggatcaggtg gcagaaattc gccctggctc ggtgatccca
    88681 gttgtagtga tgcgtgatga taagcagtta acgctgcagg tcaccattca ggaatatccg
    88741 gcaaccaatt aagtcgagtg ctcaaaacaa aaaaccggag tctgtgctcc ggttttttat
    88801 tattcgctaa tcaattactt attaacgaac tcttcgccca gggcgatatc tttcttgagc
    88861 gtatccagca taccttccag cgcgttctgt tcaaatgcgc tcagggtacc gatagattta
    88921 cgctcttcca cgccgttttt acccagcagc agcggttgag agaagaaacg ggcgtactga
    88981 ccgtcgcctt caacgtaggc acattcgaca acgccttgtt cgccctgcag tgcgcgaacc
    89041 agagacagac caaaacgtgc agctgcctgg cccatagaca gggttgcaga cccgccaccg
    89101 gctttcgctt caaccacctc agtacccgcg ttctggatac gtttggtcag atcagccact
    89161 tcctgctcgg taaaactaac gccaggaacc tgtgacagca gcggcagaat ggtaacacca
    89221 gagtgaccgc caataaccgg cacttcaact tcgcctggct gtttgccttt cagttccgca
    89281 acaaaggtgt tggaacgaat gatatccagc gtggtaacgc cgaacagttt gtttttgtca
    89341 taaacaccgg cttttttcag cacttcagca gcaattgcaa cagtggtgtt aaccgggtta
    89401 gtgataatac caatgcacgc tttcgggcag gttttcgcaa cttgctgtac caggtttttc
    89461 acgatgccgg cgttaacgtt aaacaggtcg gaacgatcca tacccggttt acgcgctaca
    89521 cctgcagaga taagaacgac atctgcgcct tccagcgccg gagtcgcatc ttcaccagaa
    89581 aaacctttga ttttcacagc agtagggata tggctcagat cgacagccac accgggagtc
    89641 actggagcga tatcatacag agagagttct gaacctgaag gcagttgggt ttttaacagt
    89701 agtgcaagcg cctggccaat accgccagca gcgccgagga ctgcgacttt catcctaaac
    89761 tccttattat attgataaac taagatatgt tgctccgctg ccgcgacctt aatccacaaa
    89821 attgccgttt acaatgacca catctcaaga atgtgtagtc acgcaagttt agcgtttatg
    89881 catttaattg ccgtaatcag gaacctaact tacgtaatta acagtcgctt atgcattagc
    89941 gcaacattcc aacaggtggt gacaatatac cctaccgttc agccaaaaca acatcaattt
    90001 gataacaatt aatttacttt taagcagaat ttgcatgccg tgacgcaggc atgtttctca
    90061 ataacgaaat ttgataaaat cccgctcttt cataacatta tttcagcctt cttcagggct
    90121 gactgtttgc ataaaaattc atctgtatgc acaataatgt tgtatcaacc accatatcgg
    90181 gtgacttatg cgaagctcgg ctaagcaaga agaactagtt aaagcattta aagcattact
    90241 taaagaagag aaatttagct cccagggcga aatcgtcgcc gcgttgcagg agcaaggctt
    90301 tgacaatatt aatcagtcta aagtctcgcg gatgttgacc aagtttggtg ctgtacgtac
    90361 acgcaatgcc aaaatggaaa tggtttactg cctgccagct gaactgggtg taccaaccac
    90421 ctccagtcca ttgaaaaatc tggtgctgga tatcgactac aacgatgcag ttgtcgtgat
    90481 tcataccagc cctggcgcgg cgcagttaat tgctcgccta ctggactcac tgggcaaagc
    90541 agaaggtatt ctgggcacca tcgctggcga tgacaccatc tttaccaccc ctgctaacgg
    90601 tttcaccgtc aaagacctgt acgaagcgat tttagagctg ttcgaccagg agctttaatc
    90661 tctgccccgt cgcttctggc ggcggggaaa atgttgctta tccctctcaa ccccctgctt
    90721 tcccctgcga ttaatttaac gaatagtgcg ttttactgcg acatgtcatt cacacaatga
    90781 atacatacgg taaaaaaagc acattatgca aaattcatta tctaattgaa aac
//
LOCUS       DAPGRN010000004        85052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPGRN010000004 DAPGRN010000000
VERSION     DAPGRN010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.004"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..527
                     /gene="cmtB"
                     /locus_tag="RHW33_000298"
     CDS             84..527
                     /gene="cmtB"
                     /locus_tag="RHW33_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV1643850.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSATDADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            555..1943
                     /gene="cmtA"
                     /locus_tag="RHW33_000299"
     CDS             555..1943
                     /gene="cmtA"
                     /locus_tag="RHW33_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV1643851.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1958..3235
                     /locus_tag="RHW33_000300"
     CDS             1958..3235
                     /locus_tag="RHW33_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV1643852.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVELAASKGIELVYVNTKGMSDPVQMLRALTGDAGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTGDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3232..4197
                     /gene="yggF"
                     /locus_tag="RHW33_000301"
     CDS             3232..4197
                     /gene="yggF"
                     /locus_tag="RHW33_000301"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV1643853.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEDVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4219..4728
                     /gene="fumE"
                     /locus_tag="RHW33_000302"
     CDS             4219..4728
                     /gene="fumE"
                     /locus_tag="RHW33_000302"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV1643854.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     EAPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4725..5438
                     /locus_tag="RHW33_000303"
     CDS             4725..5438
                     /locus_tag="RHW33_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV1643855.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PDTFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5410..6087)
                     /locus_tag="RHW33_000304"
     CDS             complement(5410..6087)
                     /locus_tag="RHW33_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1643856.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6081..6758)
                     /locus_tag="RHW33_000305"
     CDS             complement(6081..6758)
                     /locus_tag="RHW33_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV1643857.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6746..7453)
                     /locus_tag="RHW33_000306"
     CDS             complement(6746..7453)
                     /locus_tag="RHW33_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV1643858.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7454..8032)
                     /locus_tag="RHW33_000307"
     CDS             complement(7454..8032)
                     /locus_tag="RHW33_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV1643859.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKTLTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8055..8486)
                     /locus_tag="RHW33_000308"
     CDS             complement(8055..8486)
                     /locus_tag="RHW33_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1643860.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDIALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8858..9877
                     /gene="epd"
                     /locus_tag="RHW33_000309"
     CDS             8858..9877
                     /gene="epd"
                     /locus_tag="RHW33_000309"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV1643861.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGTNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTQLPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9927..11090
                     /gene="pgk"
                     /locus_tag="RHW33_000310"
     CDS             9927..11090
                     /gene="pgk"
                     /locus_tag="RHW33_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV1643862.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11305..12384
                     /gene="fbaA"
                     /locus_tag="RHW33_000311"
     CDS             11305..12384
                     /gene="fbaA"
                     /locus_tag="RHW33_000311"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV1643863.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12743..13603
                     /gene="mscS"
                     /locus_tag="RHW33_000312"
     CDS             12743..13603
                     /gene="mscS"
                     /locus_tag="RHW33_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV1643864.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13742..14377
                     /gene="argO"
                     /locus_tag="RHW33_000313"
     CDS             13742..14377
                     /gene="argO"
                     /locus_tag="RHW33_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV1643865.1"
                     /translation="MISYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14470..15210
                     /locus_tag="RHW33_000314"
     CDS             14470..15210
                     /locus_tag="RHW33_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV1643866.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15378..16274
                     /locus_tag="RHW33_000315"
     CDS             15378..16274
                     /locus_tag="RHW33_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1643867.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSQEIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16271..17749)
                     /gene="scpC"
                     /locus_tag="RHW33_000316"
     CDS             complement(16271..17749)
                     /gene="scpC"
                     /locus_tag="RHW33_000316"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV1643868.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17773..18558)
                     /gene="scpB"
                     /locus_tag="RHW33_000317"
     CDS             complement(17773..18558)
                     /gene="scpB"
                     /locus_tag="RHW33_000317"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV1643869.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18569..19564)
                     /gene="meaB"
                     /locus_tag="RHW33_000318"
     CDS             complement(18569..19564)
                     /gene="meaB"
                     /locus_tag="RHW33_000318"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV1643870.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHARHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIRDGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAETAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNIAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19557..21701)
                     /gene="scpA"
                     /locus_tag="RHW33_000319"
     CDS             complement(19557..21701)
                     /gene="scpA"
                     /locus_tag="RHW33_000319"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV1643871.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21905..22798)
                     /gene="argP"
                     /locus_tag="RHW33_000320"
     CDS             complement(21905..22798)
                     /gene="argP"
                     /locus_tag="RHW33_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV1643872.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22940..23170
                     /locus_tag="RHW33_000321"
     CDS             22940..23170
                     /locus_tag="RHW33_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643873.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23226..23885
                     /gene="rpiA"
                     /locus_tag="RHW33_000322"
     CDS             23226..23885
                     /gene="rpiA"
                     /locus_tag="RHW33_000322"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV1643874.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24140..25372
                     /gene="serA"
                     /locus_tag="RHW33_000323"
     CDS             24140..25372
                     /gene="serA"
                     /locus_tag="RHW33_000323"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV1643875.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25601..25660
                     /gene="ibsC"
                     /locus_tag="RHW33_000324"
     CDS             25601..25660
                     /gene="ibsC"
                     /locus_tag="RHW33_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV1643876.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            25993..26052
                     /gene="ibsC"
                     /locus_tag="RHW33_000325"
     CDS             25993..26052
                     /gene="ibsC"
                     /locus_tag="RHW33_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV1643877.1"
                     /translation="MMRLVIILIVLLLISFSAY"
     gene            complement(26153..26755)
                     /locus_tag="RHW33_000326"
     CDS             complement(26153..26755)
                     /locus_tag="RHW33_000326"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV1643878.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKIWEW"
     gene            complement(26776..26959)
                     /gene="ssrS"
                     /locus_tag="RHW33_000327"
     ncRNA           complement(26776..26959)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RHW33_000327"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27001..27330)
                     /gene="zapA"
                     /locus_tag="RHW33_000328"
     CDS             complement(27001..27330)
                     /gene="zapA"
                     /locus_tag="RHW33_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV1643879.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27498..28076
                     /gene="ygfB"
                     /locus_tag="RHW33_000329"
     CDS             27498..28076
                     /gene="ygfB"
                     /locus_tag="RHW33_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV1643880.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28102..29427
                     /gene="pepP"
                     /locus_tag="RHW33_000330"
     CDS             28102..29427
                     /gene="pepP"
                     /locus_tag="RHW33_000330"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV1643881.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29424..30602
                     /gene="ubiH"
                     /locus_tag="RHW33_000331"
                     /gene_synonym="visB"
     CDS             29424..30602
                     /gene="ubiH"
                     /locus_tag="RHW33_000331"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV1643882.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLQCGETLTGRVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARS
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30625..31827
                     /gene="ubiI"
                     /locus_tag="RHW33_000332"
                     /gene_synonym="visC"
     CDS             30625..31827
                     /gene="ubiI"
                     /locus_tag="RHW33_000332"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV1643883.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTCLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIIENSVIHYALWNKAHQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMLQASEDEFNRALNIAFDNRLGLCKVESERQVFPLKGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31901..31984)
                     /locus_tag="RHW33_000333"
     CDS             complement(31901..31984)
                     /locus_tag="RHW33_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643884.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            32275..33369
                     /gene="gcvT"
                     /locus_tag="RHW33_000334"
     CDS             32275..33369
                     /gene="gcvT"
                     /locus_tag="RHW33_000334"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV1643885.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33393..33782
                     /gene="gcvH"
                     /locus_tag="RHW33_000335"
     CDS             33393..33782
                     /gene="gcvH"
                     /locus_tag="RHW33_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV1643886.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDVLSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33900..36773
                     /gene="gcvP"
                     /locus_tag="RHW33_000336"
     CDS             33900..36773
                     /gene="gcvP"
                     /locus_tag="RHW33_000336"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV1643887.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILATGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLADLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(36937..38370)
                     /gene="bglA"
                     /locus_tag="RHW33_000337"
     CDS             complement(36937..38370)
                     /gene="bglA"
                     /locus_tag="RHW33_000337"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV1643888.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLRKGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAMTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            38415..38726
                     /locus_tag="RHW33_000338"
     CDS             38415..38726
                     /locus_tag="RHW33_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV1643889.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            38890..39549
                     /locus_tag="RHW33_000339"
     CDS             38890..39549
                     /locus_tag="RHW33_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV1643890.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGLVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(39626..40606)
                     /gene="ygfZ"
                     /locus_tag="RHW33_000340"
     CDS             complement(39626..40606)
                     /gene="ygfZ"
                     /locus_tag="RHW33_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV1643891.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATISGADSEKYMQGQ
                     VTADVCQMSEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVRDSQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDAATANTLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLTGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            40849..41115
                     /gene="sdhE"
                     /locus_tag="RHW33_000341"
     CDS             40849..41115
                     /gene="sdhE"
                     /locus_tag="RHW33_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV1643892.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDRLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            41096..41503
                     /locus_tag="RHW33_000342"
     CDS             41096..41503
                     /locus_tag="RHW33_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HDV1643893.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(41543..42064)
                     /gene="fldB"
                     /locus_tag="RHW33_000343"
     CDS             complement(41543..42064)
                     /gene="fldB"
                     /locus_tag="RHW33_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV1643894.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            42176..43072
                     /gene="xerD"
                     /locus_tag="RHW33_000344"
     CDS             42176..43072
                     /gene="xerD"
                     /locus_tag="RHW33_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV1643895.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQCDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            43097..43807
                     /gene="dsbC"
                     /locus_tag="RHW33_000345"
     CDS             43097..43807
                     /gene="dsbC"
                     /locus_tag="RHW33_000345"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV1643896.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            43813..45546
                     /gene="recJ"
                     /locus_tag="RHW33_000346"
     CDS             43813..45546
                     /gene="recJ"
                     /locus_tag="RHW33_000346"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV1643897.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            45637..46735
                     /gene="prfB"
                     /locus_tag="RHW33_000347"
     CDS             join(45637..45711,45713..46735)
                     /gene="prfB"
                     /locus_tag="RHW33_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV1643898.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            46745..48262
                     /gene="lysS"
                     /locus_tag="RHW33_000348"
     CDS             46745..48262
                     /gene="lysS"
                     /locus_tag="RHW33_000348"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV1643899.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(48305..48853)
                     /gene="idi"
                     /locus_tag="RHW33_000349"
     CDS             complement(48305..48853)
                     /gene="idi"
                     /locus_tag="RHW33_000349"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV1643900.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            48908..48979
                     /locus_tag="RHW33_000350"
     CDS             48908..48979
                     /locus_tag="RHW33_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643901.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(48976..49101)
                     /locus_tag="RHW33_000351"
     CDS             complement(48976..49101)
                     /locus_tag="RHW33_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643902.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(49103..50551)
                     /gene="uacT"
                     /locus_tag="RHW33_000352"
     CDS             complement(49103..50551)
                     /gene="uacT"
                     /locus_tag="RHW33_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV1643903.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            50987..52906
                     /gene="ygfT"
                     /locus_tag="RHW33_000353"
     CDS             50987..52906
                     /gene="ygfT"
                     /locus_tag="RHW33_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV1643904.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSYSDFRPRIHV
                     VGKGLAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGNIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QIDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            52906..53394
                     /locus_tag="RHW33_000354"
     CDS             52906..53394
                     /locus_tag="RHW33_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV1643905.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDCISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(53430..54797)
                     /gene="ghxQ"
                     /locus_tag="RHW33_000355"
                     /gene_synonym="ygfQ"
     CDS             complement(53430..54797)
                     /gene="ghxQ"
                     /locus_tag="RHW33_000355"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV1643906.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(54833..56149)
                     /gene="guaD"
                     /locus_tag="RHW33_000356"
     CDS             complement(54833..56149)
                     /gene="guaD"
                     /locus_tag="RHW33_000356"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV1643907.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(56167..57567)
                     /gene="xanQ"
                     /locus_tag="RHW33_000357"
     CDS             complement(56167..57567)
                     /gene="xanQ"
                     /locus_tag="RHW33_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV1643908.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(57732..60602)
                     /locus_tag="RHW33_000358"
     CDS             complement(57732..60602)
                     /locus_tag="RHW33_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV1643909.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(60599..61378)
                     /locus_tag="RHW33_000359"
     CDS             complement(60599..61378)
                     /locus_tag="RHW33_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV1643910.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(61429..62757)
                     /gene="ssnA"
                     /locus_tag="RHW33_000360"
     CDS             complement(61429..62757)
                     /gene="ssnA"
                     /locus_tag="RHW33_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV1643911.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHSRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(62760..65858)
                     /gene="ygfK"
                     /locus_tag="RHW33_000361"
     CDS             complement(62760..65858)
                     /gene="ygfK"
                     /locus_tag="RHW33_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV1643912.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAIFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPTRIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCPPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWKGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(66180..66758)
                     /gene="mocA"
                     /locus_tag="RHW33_000362"
     CDS             complement(66180..66758)
                     /gene="mocA"
                     /locus_tag="RHW33_000362"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV1643913.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKLCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            66861..67631
                     /gene="yqeC"
                     /locus_tag="RHW33_000363"
     CDS             66861..67631
                     /gene="yqeC"
                     /locus_tag="RHW33_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV1643914.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQT
                     RGRRVLITTTTHMFMPKSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            67679..69304
                     /locus_tag="RHW33_000364"
     CDS             67679..69304
                     /locus_tag="RHW33_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV1643915.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(69525..70457)
                     /gene="arcC"
                     /locus_tag="RHW33_000365"
     CDS             complement(69525..70457)
                     /gene="arcC"
                     /locus_tag="RHW33_000365"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV1643916.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(70505..71890)
                     /gene="hyuA"
                     /locus_tag="RHW33_000366"
     CDS             complement(70505..71890)
                     /gene="hyuA"
                     /locus_tag="RHW33_000366"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV1643917.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(71943..73154)
                     /locus_tag="RHW33_000367"
     CDS             complement(71943..73154)
                     /locus_tag="RHW33_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV1643918.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(73212..74408)
                     /gene="dpaL"
                     /locus_tag="RHW33_000368"
     CDS             complement(73212..74408)
                     /gene="dpaL"
                     /locus_tag="RHW33_000368"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV1643919.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(74466..75653)
                     /gene="ygeW"
                     /locus_tag="RHW33_000369"
     CDS             complement(74466..75653)
                     /gene="ygeW"
                     /locus_tag="RHW33_000369"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV1643920.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MNVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76132..77910
                     /locus_tag="RHW33_000370"
     CDS             76132..77910
                     /locus_tag="RHW33_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV1643921.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(77950..78429)
                     /gene="xdhC"
                     /locus_tag="RHW33_000371"
     CDS             complement(77950..78429)
                     /gene="xdhC"
                     /locus_tag="RHW33_000371"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV1643922.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(78426..79304)
                     /gene="xdhB"
                     /locus_tag="RHW33_000372"
     CDS             complement(78426..79304)
                     /gene="xdhB"
                     /locus_tag="RHW33_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV1643923.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRSVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(79315..81611)
                     /gene="xdhA"
                     /locus_tag="RHW33_000373"
                     /pseudo
     CDS             complement(79315..81611)
                     /gene="xdhA"
                     /locus_tag="RHW33_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            82027..82782
                     /locus_tag="RHW33_000374"
     CDS             82027..82782
                     /locus_tag="RHW33_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV1643924.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            82861..82934
                     /locus_tag="RHW33_000375"
     tRNA            82861..82934
                     /locus_tag="RHW33_000375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82893..82895,aa:Gly,seq:ccc)
     gene            83168..84778
                     /locus_tag="RHW33_000376"
     CDS             83168..84778
                     /locus_tag="RHW33_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1643925.1"
                     /translation="MKGKSALTLLLAGIFSCGTCLATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDIISNPKRLDGIPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAINTSTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKVNELDETLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLSKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            84845..85024
                     /locus_tag="RHW33_000377"
     CDS             84845..85024
                     /locus_tag="RHW33_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDV1643926.1"
                     /translation="MINKLLLAYLIGLVVTSTLIFIFSEEKVTYRLFAAIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        20759 a        21057 c        22407 g        20829 t
ORIGIN      
        1 gtacccaatt gctttcatta aaccgcacga gtattttatt tttaccgccg gacggtaccc
       61 taaaaataaa ataaggataa tttatgcggc ttagtgatta ttttccagaa tcatcaatct
      121 cagttataca ttcagcaaaa gattggcagg aagctatcga tttctcgatg gtatcattgc
      181 tggataaaaa ctatatcagc gagaattaca ttcaggcaat taaagattcc accattaaca
      241 atggccctta ttatattctc gcaccaggcg tggcaatgcc tcatgcgcga ccggaatgtg
      301 gggcgcttaa aaccgggatg tctttgacat tacttgaaca aggtgtttat tttccgggga
      361 atgacgaacc aattaaatta ctcatcggac tctctgctac cgatgccgat tcgcacattg
      421 gcgctattca ggcgttaagt gaattactgt gcgaagaaga aatactcgaa caactcttaa
      481 cagcatcatc agaaaaacaa ttagcggaca ttatcagccg cggataatta ctctctcctc
      541 tacaggataa tattatggaa aacaagtctg ctcgtgcaaa ggtccaggct tttgggggct
      601 ttttgactgc aatggtcatc cccaatattg gtgcttttat tgcctggggt tttattactg
      661 cgttatttat tcccaccggt tggctgccta acgaacattt cgccaaaatt gtcggcccga
      721 tgattaccta tttattgccc gtgatgattg gttctaccgg tggtcatctg gtcggcggta
      781 aacgcggcgc ggtcatgggc ggaataggta ctattggtgt gatcgtcggc gcagagatcc
      841 cgatgttcct tggctcaatg attatggggc cgctcggtgg tctggtcata aaatatgttg
      901 ataaggcact ggaaaaacgc atacctgccg gttttgagat ggttatcaat aacttctcat
      961 taggtatcgc ggggatgctc ctttgcctgc tgggttttga agtgatcggc ccggcggtgt
     1021 taattgccaa tactttcgtc aaagagtgta ttgaggcgct ggtacatgcg ggttatctgc
     1081 ctctgctgtc agtcatcaat gaaccggcga aagtgctttt ccttaataat gcgatcgatc
     1141 agggcgtcta ttatccgctg ggaatgcaac aggcttcggt taacggtaaa tccatcttct
     1201 ttatggtggc ctctaaccca ggtccgggcc tggggctgct gctggcgttt accttgtttg
     1261 gtaaagggat gagtaaacgt tctgcgcccg gggcgatgat tattcacttc ctcggtggga
     1321 tccacgaact gtatttccca tatgtgctga tgaagccgct gactattatt gccatgattg
     1381 cgggcggtat gtctggcacc tggatgttta acttactgga cggtggtctg gtggctggcc
     1441 caagtccggg gtctatcttt gcttacctgg cgctgacgcc gaaaggttcg ttcctggcga
     1501 caattgccgg tgttacggta ggtaccctgg tgtcctttgc tatcacttcg ctgatactga
     1561 agatggaaaa aacggtggaa acggagagcg aagatgagtt tgctcagtca gccaatgcgg
     1621 ttaaggcgat gaaacaagag ggtgcattct cgttaagcag ggttaagcgt atcgcctttg
     1681 tttgcgatgc ggggatgggc tccagtgcga tgggcgcgac caccttccgt aaacgcctgg
     1741 aaaaagcggg gctggcaatt gaagtaaaac attacgccat agaaaacgtg cctgcggatg
     1801 cggatatcgt cgttactcat gccagtctgg aagggcgcgt gaaacgtgtg acggataaac
     1861 cactgatatt gattaataac tatattggcg atccaaaact cgacacttta tttaatcaat
     1921 taaccgccga acataaacac tgattacaga ggtaaaaatg aaaaccaaag ttgctgctat
     1981 ttatggcaag cgggatgtcc gtctgcgcga atttgaactg ccagaaatta ccgataatga
     2041 attactggtg agtgtgattt ctgacagcgt ctgtttatcc acctggaaag cggcgttact
     2101 cggtagtgaa cataaacgcg tacccgacga tttagaaaat catccggtca ttaccgggca
     2161 tgaatgtgcc ggggttattg tcgaagtggg taaaaatctc actggcaaat ataaaaaagg
     2221 ccagcgtttt gtattgcaac cggcgatggg gttaccaagc ggatattcag cgggctacag
     2281 ctacgaatat tttggcggca atgccactta tatgattatt cccgaaatcg ccattaattt
     2341 gggctgcgta ttaccgtatc acggctctta ttttgctgcg gcgtcgctgg cagagcctat
     2401 gtgctgcatt attggtgctt atcatgccaa ttatcacacc acgcaatatg tttatgagca
     2461 tcgcatgggc gtcaaacctg gcggtaatat tgcactgctg gcgtgtgcag gtccgatggg
     2521 cattggcgct atcgattacg ccattaacgg cggcatacaa ccgtcgcggg tggtggtggt
     2581 cgatatcgac gacaaacgtc tggcgcaggt acagaagctg ctgccggtgg aactggcggc
     2641 cagtaaaggc attgagctgg tgtatgtgaa taccaaaggg atgagcgatc ctgtccagat
     2701 gctgcgggcg ctgacaggag atgccgggtt cgatgacatt tttgtttatg cggcggtgcc
     2761 tgctgtcgtt gagatggctg atgaattact ggcggaagat ggctgtctga acttctttgc
     2821 cgggccgacg gataaaaact tcaaagtgcc gtttaatttc tacaacgttc attacaacag
     2881 cacgcacgtc gtcggtacat ccggcggttc aacgggcgac atgaaagagg cgattgccct
     2941 tagcgccact gggcagttac agccgtcgtt tatggtgacc catatcggtg gcctggatgc
     3001 ggtgccagaa accgtgctca atctgccgga tatccctggc ggtaaaaaac tcatttataa
     3061 cggcgtgacc atgccgctca ctgccattgc cgattttgcc gaaaaaggca aaaccgatcc
     3121 gctgtttaaa gagttggcgc ggctggttga ggaaacgcac ggcatctgga atgaacaggc
     3181 cgagaaatat ctgctggcac aatttggcgt tgatatcggg gaggccgcgc aatgatgtcc
     3241 ctggcgtggc cattatttcg cgttacggaa caggcagcgt tggctgcctg gccgcaaacc
     3301 ggatgtggcg acaaaaataa aattgatggc ctggcggtca ccgcgatgcg ccaggcatta
     3361 aacgacgtcg cttttcgtgg acgagtggtt atcggcgaag gagaaattga ccatgcgccg
     3421 atgctgtgga ttggcgaaga tgtgggcaaa ggcgatggac cagaagtcga tatcgcggtt
     3481 gatcccattg aaggcacgcg gatggtggcg atggggcaga gcaatgcgct ggcggtcatg
     3541 gctttcgccc ctcgagatag cctgttacat gcgcccgata tgtatatgaa aaagctggtt
     3601 gttaatcgac tggctgccgg ggcgattgat ctgtctttgc ctctggcgga caacttgcgt
     3661 aacgtggcgc gggcgttagg taaaccgctg gacaagctgc gcatggttac gctggataaa
     3721 ccgcgtctga gtgccgcgat tgaggaagca acacagctgg gggtgaaagt ttttgccctg
     3781 ccggatggcg atgtcgccgc cagcgtgctg acctgctggc aggataatcc ttacgatgtg
     3841 atgtacacca tcggcggtgc gccagaaggc gtgatttctg cctgtgccgt taaagcatta
     3901 ggcggggata tgcaggccga gctgattgac ttttgtcagg ctaaagggga ttacacggaa
     3961 aatcggcaga ttgcagagca ggagcgcaag cgttgcaagg caatgggcgt tgacgttaac
     4021 cgtgtgtact cgctcgatga actggtgagg gggaacgata tcctctttag cgctacgggc
     4081 gtcacgggcg gcgagttggt gaacggtatc caacagacgg cgaatggggt gcggacgcag
     4141 acattactga tcggcggcgc ggaccaaacg tgtaatataa tagactccct gcattgatgg
     4201 tgatctatcg gaagaccaat ggcgacgctg acagaagatg atgtgcttga gcaactggat
     4261 gcacaggaca atttattttc atttatgaaa actgcgcatt ctattttgct ccaggggata
     4321 cgccagtttc tgccgtcgct gtttgtcgat aacgatgaag agatcgtcga atatgcagtg
     4381 aagccgttac tcgcccaaag cggcccgctt gacgatattg atgtcgcact gcgtttgatt
     4441 tatgcgctgg ggaaaatgga taaatggctg tacgccgata tcacgcattt ttcccagttc
     4501 tggcattacc tgaacgaaca ggatgaagcg cccggatttg ccgatgacat gacctgggat
     4561 tttatcagca acgtcaatag cataacctgc aatgcgacgc tctatgacgc gttgaaagcg
     4621 atgaagttcg ccgatttcgc cgtctggtca gaggcgcgtt ttagcggaat ggtcaaaacg
     4681 gcgctgacgc tggcagtaac gaccacctta aaggaattaa cgccgtgaaa attgaattaa
     4741 cggtgaatgg gctgaacgtg caagcgcagt accatgatga agagatagaa cgcgtgcata
     4801 aaccgttgct gcgtatgctg gcggccttac agacggtaaa tccgcagcgc cgaaccgtgg
     4861 tttttctttg cgctccgccg gggacgggga aatctaccct aaccaccttc tgggaatacc
     4921 tcgcgcaaca agacccggaa ctgcccgcta ttcagacgct gccgatggat ggttttcacc
     4981 attacaatag ctggctggat gcgcatcaat tgcgcccctt caaaggcgca ccagatacat
     5041 tcgatgtggc gaaactggcg gaaaatctgc gccaggtagt ggaaggggat tgtacgtggc
     5101 cgcaatacga tcgacaaaag catgatccgg ttgaagatgc gttgcacgtt accgcaccac
     5161 tcgtcatcgt cgaaggaaac tggttgttgc tggacgacga gaagtggtgc cagcttgcgc
     5221 aattttgtga tttctcgatc tttatcaacg cgccagctac tgcgttgcgg gaacgactgg
     5281 tagggcgcaa gctggcggga ggggtatcgc tggcagatgc agaagctttt tatgaccgta
     5341 ccgacgggcc gaatgttcgc cgggtgctgg aagagagtct gcctgcaaat ttgactttaa
     5401 tgatgaccgc tacgggtgaa tatcgtctgg tggattaacg ttcctgatta gaccatcttc
     5461 cagtcgcacc acacgagaga aatgacgccg ggtaaatgcg gcgtcatgac tgattgctac
     5521 gacgctcgtg cctcgctggc ggcacttctc cagccagctt tcaaacacgc tcaaccaatt
     5581 ttcgtcaaaa tcccgactcg gctcgtccag cagcaatagt ggcggagaga gtgcttccag
     5641 gcaggcgacg gcgaccatcc gtcgctgcgc actatgcaga tccaacgggt gcgcgttggc
     5701 agtgtctgct aactgacaac attgcagggc agcatgggta cgttgagtga tttcatccgc
     5761 cgggcatttt tgcagtttca aaccaaatgc gatctcgtca gcgacggtgc tgtgaaaaag
     5821 ttggttttcc gcttcctgaa acagtacgcc aatctttgcc gcgcgctggc ggtttttcag
     5881 gtttttcatc gcctgttgtt gcagcataac cgtaccggct gtgggcgtaa gaagacctgc
     5941 cattactcgt aacagcgtcg atttccctgc gccattgtcg ccggtcagcg ccaaccattc
     6001 accttgtttg agttgcagag aaatgtcgca aagacaatcc gttgccgcgc caggccagcg
     6061 ataagatatc tgatttaacg ttaacatagt ggctggagtg ctccgttttg caattgccac
     6121 gcttgctggc agtagttcag gaaaggtgtg cgatgacgtt caaaaagaat aatcagcgaa
     6181 ccacgttcaa acgcccagtg ttgtaagcgc tgcaacagca tttcgctggc ctgcggcgtc
     6241 aggcggctaa aggcttcatc aagaattaat agctttggct gcatcgcgat ggcgcaggcg
     6301 atgaccacgc gctgtgtctc tccaccggag agtgttgccg ggtgacgatg gcgcaacggc
     6361 tggcactcgg tcagagtcag ggcggcatca atccgcgcca taatctctgc ttcagcaaga
     6421 cacagatttt ctggcccaaa ggcaacttct tcttccacgc taaaggtaca gccggagagc
     6481 tgcaaatagg gcgattgctg gacgagctgg atggtggcgg attgttcatt aagcgggaga
     6541 tgcccaatgg gcgtaccgag gagcgtcccc gtgcctgtta tctctcctgg cagaaagtcc
     6601 ggataccagc ccgccatcag ctgtgccagc gtacttttgc cactgccgtt gtcaccaaag
     6661 atggcgacca tccccggttt gacatagtga aagtcataac agaacggagg atgcgttgag
     6721 tgtgtcgggc aataacggaa ctgttctaac gtaaccatat ccacgctccg aattctgtca
     6781 gcatcagcaa gatcatggtg taacgcgcta cgcgttgtag ggtgctatcg gctggtgccc
     6841 acaacgttgt tcgtcggttg tggatccgaa atgcccgcat atccagcgcc gcaccacgga
     6901 cagcaagatc gtttagcgca ttgtgtgtta gcggaataat taacgcgggc atcgcacgta
     6961 aacgctgata ccagccctca tccagcggca cgccgcgggc acgctgcgct tcatgaataa
     7021 tcgctaattg ccgttttaac tgctcaacga ccaataacgg cccggcaaac agataagcga
     7081 cgcctggcgg caggcgagaa gcaaacagag cgcggataaa acattgcacg gggacgtact
     7141 gcatccacaa ctgtgaggtt gaaacaatcg ccagaatccg caaccagagc gtaatagcat
     7201 gcgtccaacg ctccggcgaa cgcggcgtgc cgcttagcca ttctgttagc cagccaccgt
     7261 gtaccagcca cagcccggct cccagcgaga acatcagcca ggcgacatat tttgcccgcc
     7321 gccgcgtggc ctttagcgca ataaggcaaa aaaaggtcgc cgcgctatag atcggcaaaa
     7381 tggtctgcgc gggcaggatg agcgtggtgc aggccgccaa tgcccatagt gttaatgagg
     7441 taaatggatg catttagcgc acggcagcca ttgccggaaa atggcgcgtg gtgcgaatcg
     7501 gcagctggcg cagtaaaagc cagacaatca ccgctgtaag agttttatcg accaggttcg
     7561 cgcctatcac cgtaatcgcc accgactcca ccaggttttg ccccatcgag tgcatccagg
     7621 caacgaacaa atcggctccg ctaccggtga caccgccgaa cagcgcagta cgcagcggta
     7681 cagcgacgac cgttaccgca agcgtaataa tcacgccgct gacaacgact tttggtaggg
     7741 tgcgaaacca acccgctcgc gccagccaac cggcgaccag gccgatgacc atcgcgacgg
     7801 gtgcaaatgc ggcggcgatc gggtcggtca gcaatcccca gagtaaattg gtcagtaagc
     7861 cggtcagcat tccgatcacc ggaccaagca gtaccgcgct aataagtgtg ccgatggagt
     7921 cgagaaatat aggtaatttg accatgctgg ccagttgccc gccgatcata ttgatggcga
     7981 tagatataac gattaacacc agcgcctgac tggaaaagtg gctacgcgcc ataagaaaac
     8041 ctctctgaaa gtagttattt aacgggggaa atatgcaact cgtcgtaacc agaaagcgcg
     8101 caaggttggc ggctaaacgc cagttcctca aggctaccga tcaccagttc aagcgtggtc
     8161 cgcacggtac agccgggcat catatgaccg ccaagcatcg tgccgtgtgg atcggaaacg
     8221 cagagatgaa gatgctcacc actctgttcc aacgtgccgt ttagtgagat cacttcgaat
     8281 gtaccgttca ggagcgtggt gccttcttgc ccggcatagc gcaaggcgat gtcagtcagg
     8341 ctaccggtac agcctgcaat ccaggcggca tgaagttgct gctgctgtgc aaaagcgtgt
     8401 agctgggaaa aaacttcctg cccggggagg aggcgcagtg cgtaaaagcg tgcggaggaa
     8461 gaatatgggt agggcaatgt catcattgga gtactccgaa actcggacgt attgcggata
     8521 atatcgacgc tgcatcagtc atattctgat ataagtcagg gtttgtgacg gcacaacgaa
     8581 aagattttgc tacgcttatt cgtgattctg caaagtggaa taaattccac aggggcgggg
     8641 ctgaaaacgt gacgtaagtc agatatttgt tttctttgac tggacaatca ttccttttat
     8701 tccacgtttc gcttatccta gctgaagcgt ttcagtcgat taaatgttcg acaattaacc
     8761 aatcagtcgc agtttgcgac aggtaaggtt tccccggacg atttgctgga ttactctgtc
     8821 tggcaaattt gctcagggaa aaccttgcag gagatctatg accgtacgcg tagcgataaa
     8881 tggcttcggt cgcatcgggc gtaatgtggt tcgtgctttg tatgaatccg gacgccgggc
     8941 ggaaattacc gtggtggcaa tcaacgaact ggcggatgct gcgggcatgg cgcatttgtt
     9001 gaaatatgac accagccatg gccgttttgc atgggaagta cgacaggaac gcgatcaact
     9061 ttttgttggt gatgacgcca tccgcgtatt gcatgaacgt tcactgcaat cgctcccctg
     9121 gcgtgaactt ggcgttgatg tagtcctcga ctgcaccggc gtatatggct cccgcgagca
     9181 tggcgaagcg catattgccg ccggggccaa aaaagtgctc ttttcacatc ctggcactaa
     9241 cgatctcgac gcgaccgttg tttacggcgt caatcaggat caacttcgtg cggaacaccg
     9301 catcgtttct aacgcttcct gtaccacgaa ttgcataatt cccgtcatca aattgttaga
     9361 tgatgcgtac ggtattgagt ccggcactgt gaccacaatt cactccgcca tgcacgatca
     9421 acaggttatt gatgcatacc atcctgacct gcgtcgcacc cgggcagcca gccagtcgat
     9481 cattccggtc gatactaaac tggccgccgg tatcacacga ttttttccgc aatttaacga
     9541 tcgctttgaa gcgattgcgg tacgtgtgcc aaccataaat gtgacggcaa tcgatttaag
     9601 cgtgacggtg aagaaacctg taaaagccaa tgaagtcaac ctgttgctgc aaaaagcagc
     9661 acaaggtgca tttcatggta tagttgacta tacgcaattg ccgttggtct ctgtagattt
     9721 taaccacgat ccgcacagtg ccattgtcga tggcacccaa acccgggtca gtggcgcaca
     9781 cctgatcaaa acgttggtct ggtgcgataa cgaatggggc tttgctaacc gaatgctcga
     9841 cacgacgtta gctatggcta ctgttgcttt caggtaagac gcaagcagcg tctgcaaaac
     9901 ttttagaatc aacgagagga ttcaccatgt ctgtaattaa gatgaccgat ctggatcttg
     9961 ctgggaaacg tgtatttatc cgtgcggatc tgaacgtacc agtaaaagac gggaaagtaa
    10021 ccagcgacgc gcgtatccgt gcttctctgc cgaccatcga actggccctg aaacaaggcg
    10081 caaaagtgat ggtaacttcc cacctgggtc gtcctaccga aggcgagtac aacgaagaat
    10141 tctctctgct gccggttgtt aactacctga aagacaaact gtctaacccg gttcgtctgg
    10201 ttaaagatta cctcgacggc gttgacgttg ctgaaggtga actggttgtt ctggaaaacg
    10261 ttcgcttcaa caaaggcgag aagaaagacg acgaaaccct gtccaaaaaa tacgctgcac
    10321 tgtgtgacgt gttcgtaatg gacgcattcg gtactgctca ccgcgcgcag gcttctactc
    10381 acggtatcgg taaattcgct gacgttgcgt gtgcaggccc gctgctggca gctgaactgg
    10441 acgcgctggg taaagcactg aaagaacctg ctcgcccgat ggtggctatc gttggtggtt
    10501 ctaaagtatc taccaaactg accgttctgg actccctgtc taaaatcgct gaccagctga
    10561 ttgttggtgg tggtatcgct aacaccttta tcgcggcaca aggccacgat gtgggtaaat
    10621 ccctgtacga agctgacctg gttgacgaag caaaacgtct gctgaccacc tgcaacatcc
    10681 cggttccgtc tgatgttcgc gtagcaaccg agttctctga aactgcacca gctaccctga
    10741 aatctgttaa cgatgtgaaa gctgacgagc agatcctgga tatcggtgat gcttccgctc
    10801 aggaactggc tgaaatcctg aagaatgcga aaaccattct gtggaacggt ccggttggcg
    10861 tgttcgaatt cccgaacttc cgcaaaggta ctgaaatcgt ggctaacgct atcgcagaca
    10921 gcgaagcgtt ctccatcgct ggcggcggcg acactctggc agcaatcgac ctgttcggca
    10981 ttgctgacaa aatctcctac atctccactg gcggcggcgc attcctcgaa ttcgtggaag
    11041 gtaaagtact gcctgcagta gcgatgctcg aagagcgcgc taagaagtaa aaaatcacag
    11101 ggcagggaaa cctgcccttg tttcagcgcg cttttagagc acgcattttt tcatactcta
    11161 aataattcga gttgcaggaa ggcgacaaga gagtgaatcc ccaggagctt acataagtaa
    11221 gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtataag
    11281 gcccgacgat acaggacaag agacatgtct aagatttttg atttcgtaaa acctggcgta
    11341 atcactggtg atgacgtaca gaaagttttc caggtagcaa aagaaaacaa cttcgcactg
    11401 ccagcagtaa actgcgtcgg tactgactcc attaacgccg tactggaaac cgctgctaaa
    11461 gttaaagcgc cggttatcgt tcagttctcc aacggtggtg cttcctttat cgctggtaaa
    11521 ggcgtgaaat ctgacgttcc gcagggtgct gctatcctgg gcgcgatctc tggtgcgcat
    11581 cacgttcacc agatggctga acactatggt gttccggtga tcctgcatac cgaccactgc
    11641 gcgaagaaac tgctgccgtg gatcgacggt ctgctggacg cgggtgaaaa acacttcgct
    11701 gctaccggta agccgctgtt ctcttctcac atgatcgacc tgtctgaaga atctctgcaa
    11761 gagaacatcg aaatctgctc taaatacctg gagcgtatgt ccaaaatcgg catgactctg
    11821 gaaatcgaac tgggttgcac cggtggtgaa gaagacggcg tggacaacag ccacatggac
    11881 gcttctgcac tgtacaccca gccggaagac gttgattacg catacaccga gctgagcaaa
    11941 atcagcccgc gtttcaccat cgcagcgtcc ttcggtaacg tacacggtgt ttacaagccg
    12001 ggtaacgtgg ttctgactcc gaccatcctg cgtgattctc aggaatatgt ttctaagaaa
    12061 cacaacctgc cgcacaacag cctgaacttc gtattccacg gtggttccgg ttctactgct
    12121 caggaaatca aagactccgt aagctacggc gtagtgaaaa tgaacatcga taccgatacc
    12181 caatgggcaa cctgggaagg cgttctgaac tactacaaag cgaacgaagc ttatctgcag
    12241 ggtcagctgg gtaacccgaa aggcgaagat cagccgaaca agaaatacta cgatccgcgc
    12301 gtatggctgc gtgccggtca gacttcgatg atcgctcgtc tggaaaaagc attccaggaa
    12361 ctgaacgcga tcgacgttct gtaagatatt cctttctgct tatctcaagg cccgctctgc
    12421 gggtcttttt tttgccaaaa gaaaaattgc ctgatgcgct acgcttatca ggcctacacg
    12481 tatcctgcaa tatattgaat tcgcaggttt ttgtaggccg gataaggcgt tcacgccgca
    12541 tccggcatga caaagcgcac attgatatct gtctgagccc cgctctgcgg ggcttttttt
    12601 cgccaaaagc aaggtgatac agatgagaaa tctgtgatct atttggcaaa attatgcttt
    12661 attgtttacc cttgtcagac tgcccgtcat aaggcggcgg agtgtatttc tccattttga
    12721 gtcagttgaa aaggaatatt gaatggaaga tttgaatgtt gtcgatagca taaacggtgc
    12781 gggaagctgg ctggtggcta accaggcgct gctgctaagt tatgcagtaa acatcgtggc
    12841 ggcactcgcg atcatcatcg ttggtttgat tatcgcgcgg atgatttcca acgcggtgaa
    12901 tcgcctgatg atctcccgta aaatcgatgc cactgttgct gattttcttt ctgcattagt
    12961 ccgttacggt attatcgcct ttacgctaat cgctgcactg ggacgcgtgg gtgtacaaac
    13021 cgcgtccgtc attgctgtac tcggtgccgc aggcttagct gttggtctgg ctttgcaggg
    13081 gtcactttct aacctggccg ctggcgtgtt acttgtcatg ttccgcccgt tccgtgccgg
    13141 agaatatgtt gacctcggcg gcgtagccgg tactgtgctg agtgtgcaga ttttctccac
    13201 caccatgcgt actgcagacg gtaaaattat cgttattccg aacggtaaaa ttattgccgg
    13261 aaatattatt aacttctccc gcgagccagt tcgccgtaac gagtttatta ttggcgtggc
    13321 gtatgattcc gatatcgatc aggttaagca gatcctgacc aatattatcc agtctgaaga
    13381 tcgcattttg aaagatcgcg aaatgactgt gcgcctgaac gaacttggtg catcgtcgat
    13441 taatttcgtg gtccgcgtct ggagcaacag cggcgatctg caaaacgtgt actgggatgt
    13501 gctggagcgt attaaacgtg aatttgatgc cgccggtatc agcttcccgt acccgcaaat
    13561 ggatgtgaac tttaagcggg tgaaagaaga caaagctgcg taatcaacgc tgaggccaga
    13621 taatactttc tggcctctct cttattagtt tttctgattg ccaattaata ttatcaattt
    13681 ccgctaataa caatcccgcg atatagtctc tgcatcagat acttaattcg gaataccaaa
    13741 cgtgatttct tattactttc aaggtcttgc acttggtgct gctatgatcc taccgctcgg
    13801 tccacaaaat gcttttgtga tgaatcaggg cattcgtcgt cagtaccaca ttatgattgc
    13861 cttactttgc gctatcagcg atttggtcct gatttgcgcc gggatttttg gtggcagcgc
    13921 gttattgatg cagtcgccgt ggttgctggc gctggtcacc tggggcggcg tagccttctt
    13981 gctctggtat ggttttggcg cttttaaaac agcgatgagc agtaatattg agttagccag
    14041 tgccgaagtc ctgaagcaag gaagatggaa aattatcgcc accatgttgg cagtgacctg
    14101 gctgaatccg catgtttacc tggatacttt tgttgtactg ggcagccttg gtgggcagct
    14161 tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt agcgcctctt tcctgtggtt
    14221 ctttggtctg gctattctcg cagcctggct ggcaccgcgc ctgcgcacgg caaaatcaca
    14281 gcgcattatc aatctggtag tgggatgtgt catgtggttt atagccttgc agctggcgag
    14341 agacggtatt gctcatgcac aagccttgtt cagttaggcg ctatctgatg gaaaaataaa
    14401 acagaggcgc taagcttgcc tccagaggtc ctgaattttt ggcaagtcaa ttaacacgga
    14461 gagactaacg tgaagttcaa agttatcgcc ctggcggcat taatgggtat tagcgggatg
    14521 gcagcgcagg ctaacgaatt gccggatgga ccgcatattg tcacctccgg tacggcaagc
    14581 gtggatgcgg tgccagacat tgccactctt gcgattgaag ttaacgtggc cgcgaaggat
    14641 gccgctactg ccaagaaaca ggcagatgag cgcgtcgcac aatacatttc cttccttgaa
    14701 ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaact tacgcaccca gccagattat
    14761 gattatcagg atggtaaaag tatcctgaaa ggctaccgcg cagtgagaac ggtggaagtc
    14821 acgctccgtc agttagacaa actgaattcc ttgctggacg gcgcgctgaa ggcgggtctt
    14881 aacgaaattc gttctgtgtc gctgggcgtg gcgcagccgg atgcctataa agacaaagcg
    14941 cgtaaggcag cgattgataa cgcgattcat caggcgcagg aactggcgaa cggctttcat
    15001 cgtaaactgg ggccggtata tagcgtgcgc taccatgttt ccaactatca gcccagccca
    15061 atggtgcgga tgatgaaagc cgatgccgcg ccggtgtccg cccaggaaac ttacgagcag
    15121 gccgctattc agtttgatga tcaggtcgat gtggtcttcc agttagaacc tgtggatcaa
    15181 caacccgcta aaacacctgc agcacaataa tacttgtagc ccgacgtatt cgtcgggcta
    15241 atgcctttac ccgatattgc ctcctttttc tgtaattttc tgtaaaacca tgacgtaatc
    15301 acctatcttt gctcgcggtt tcatcaagga taggctgact gaaacggtta agcggacaag
    15361 gatgcggggg aggggggatg gacattttta tttcgaaaaa gatgcgcaat ttcattttat
    15421 tagcgcaaac caataatatt gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat
    15481 ttggtaaaag cattgccgcg ctggaagaac aaattggcta tacgctattt acccgcaaag
    15541 ataataatat cagcctcaat aaagccggac aggaactgta tcaaaaactg ttcccggttt
    15601 atcaacggct ttctgcgatt gataatgaaa tccataactc agggcgtcgt tcacaagaaa
    15661 ttgtgattgg catagataat acttatccga caattatttt cgatcagttg attagtctcg
    15721 gagataagta tgaaggcgtg accgcccagc cagttgagtt cagcgaaaac ggcgttatcg
    15781 ataatctttt tgaccgccag ttagatttta ttatctcccc acagcatgta tctgcacgag
    15841 ttcaggaact ggaaaacctg actatcagtg aactaccacc gctgcgatta ggctttctcg
    15901 tttctcgccg ttatgaagag cgtcaagagc aagagctgct tcaggagctg ccctggctac
    15961 aaatgcgctt tcagaaccgg gccaacttcg aagcaatgat tgatgcgaat atgcgacctt
    16021 gtgggatcaa tccaaccatt atttatcgtc cctacagttt tatggccaaa atcagcgccg
    16081 tggagcgcgg acattttctg acggtgatcc cgcattttgc ctggcgactg gtgaatccgg
    16141 caacgctgaa atattttgat gcgccgcaca ggccgatgta tatgcaggaa tatctttatt
    16201 caatcagaaa tcatcgttat accgccacga tgcttcagca tattgctgaa gatcgtgacg
    16261 ggacgagtca ttaacccagc atcgagccgg ttgcaattaa attacggtgt aagtcgaaga
    16321 cgtggctaag atcgtggtga atatgtccgc caggcgcatt ttccagatag cgatgcagat
    16381 aatcccgata cataggatgt gcacaattat caatgatcgt gcgggcgcgt tgaagcgggg
    16441 aaagaccgcg cagatcggcg atcccttgtt cggtgatgat cactttgacg ctgtgttcgc
    16501 tgtgatcaac atggctgcac attggcacga cggttgagat cttgccttct ttagcaatcg
    16561 acggggccat aaagatcgac agatacgcgt tgcgttcaaa atcaccgctg ccgccgatgc
    16621 cgttcatcag atcgaccccg gctacgtgcg ttgagttggc atgcccgtaa atatcaaact
    16681 ccaggccgac gttcagagcg atgacgccca gacgacggat gatttccggg ttattggaaa
    16741 tctcctgcgg acgcaacaca atgcggctgg caaagtaatc catattgtcg taaatcttgc
    16801 gcagggaatc ggccgagatt gtcaggctgg aggcgctggc cccgctgatt ttgccggttt
    16861 ccagtaaatg caccaccgat tcctgtagca cttccgaata catcataaac ggaggaattt
    16921 ccgggttttc ccccagacgc gccattaccg cattattgat attgcccacg ccactttgca
    16981 gcggcagaaa ttccggcgga atacgcccat gcgccatttc ctgcaataag aacgtgacca
    17041 cgttatcggc aatctgctgg cacatgggat tttgcttatc cagcatatta ccggcgtcgg
    17101 gcaagttggt ttccacgacg gcgacaatct ttttcggatc gatttgcaca tagcgggtac
    17161 cgacgcgatc cattgcatgg aagatcgaca cgctattgcg ccgcggtggc gcgccaggaa
    17221 tcacaatatc cgccagttct gcaacgcgcg gatcgtgata gtgattgagt tcaatgatca
    17281 ctttcttcgc tcgcagcagc caggtcggcg cattaccgat cccgctggtt aaccagactc
    17341 gaccatctgg tgccagtgcc gatgcttcaa tgacggcaac atcaatgtcg ccgaagaaac
    17401 cgtaattgac catttgcgcc acttcgctca aatgcaggtc aacgaaactc accgcgccct
    17461 gattgatctt tttacgtaaa ccggacgatg tttgatatgg cgcacgccag gaaacagcat
    17521 cggcgtcaga aagtacatcg tcagcggcgg cgctgattga agcccccgtc agcaggcgaa
    17581 tttgatacgg ctttttggcc tcatgctgtt cgttagctct gcgggcaatc gcggtgggta
    17641 gggctttcgg tgaacccgct ggggtaaagc cgctaaatgc caccatgtcg ttatgctgga
    17701 taatttctgc cgcttcatcg gcggtcatcc ttgtccactg agtttccatt tttactcctt
    17761 cgttcgcagg gattaatgac caacgaaatt aggtttacgt ttttcgagga aagcgttcat
    17821 cccttcctgg taatcttcgc tgtcataaac cgcgcggcgc atcccctgaa tacgttcaaa
    17881 ttcatcggag ttcatggtgt gtgcttcgcc cagtacacgc agctcttctt tgataacggc
    17941 aatggctaac ggcgctttct cagagatgtg gtgcgccatt tgtaaggtga aatcttccag
    18001 ttcttccact tccacaacat ggttgaggat gccgacagcc agcgcgcgct gggcggtgat
    18061 tggcgaagcg gtaaaaatca gctctttgac aatgtggaag cccgcgtcgc gggtcaggtt
    18121 gtgaatgccg accaggttat acgggacgcc gaggtttaca ggcgtcattg agaaggttga
    18181 ggtactggcg gcgatgatca gatcggaact catgatcatt tcaaatgcgc caccccaaac
    18241 actaccttcc accatcgaaa tgatcggttt cgggaatttt tggatcatgc gggtgatttg
    18301 acgcaatgga tcatcatagg agagcggatc gcgaccgcca gacggcagtt cgtgaatatc
    18361 gtgacctgcg gagaagactt tggatccact cggcgcgcgc aaaatgatac agcgaatttc
    18421 cggccggttg agatcgctta acgcctgcat aagatcatca ataaagactt tacttaaggc
    18481 attaagtttt cggccatagt taaactcaat gaccgccact ttgttgatag tggcaacgtt
    18541 cacatactga taagacataa aaattccttt aatcaaaata ttgcgtctgg ataaattcac
    18601 tgagctgccg caggccggtg cgcggtgaga gcgtattgtt tttgaccgct aaaagcgtct
    18661 ggcggtaata gcgatcgaaa tcttcattcg cgaacaggtg attcagtact tcttcttcgg
    18721 tctgcttacg cagccattcc accgattgtt gttgccgcac ttgttgtaaa cgaccactgg
    18781 cagttagcgc ggttttgaag tcgatgatgg cgtgccagat ctcatcgatt ccacgttttt
    18841 ccagtgcgct acaagtcaga acccgtggct gccattcgtc gtatttacgt cgcagaatat
    18901 gcagggcact ctcgtacata tgccgggcaa tggcgatatt ggtatggtta tcgccatcgt
    18961 ctttgttgat tacgatcaga tcagccactt ccatcagccc ttttttaatg ccctgcagat
    19021 catcgccgcc accggcaatt tgcaacgaga taaaacagtc caccatgcgg gcgacttccg
    19081 tttccgactg cccgacgcca accgtttcga caatcactac gtcataaccc gccgcttcgc
    19141 ataacagcat taattcccgc gctcgctgac tggcaccgcc cagatgaccg gaggatggta
    19201 ccgggcgaat aaacgccgtc tcggcacgcg ccaggtcatt catgcgggtt ttatccccga
    19261 gaatgctacc gccagtgacc ggactgctgg gatcgaccgc aataaccgca acctttaatc
    19321 catcacgaat caacaacatg ccaaaggctt caagaaaggt acttttcccc gcgccgggtg
    19381 tgccggtaac gcccagtcgc agggcgttac cgcagtacgg cataatggca tcaagcagtt
    19441 gcgtacttag cgcctgatga cgcgcgtgac ggctttccac cagcgtcatg gcctgggcga
    19501 gtgtggcacg ctcaccctga cgtaagcggc gaatactttc tgccagcgtg gcttcattaa
    19561 tcatgatgct ggcttatcag attcagtacg tcgcgcacac tgtcgagcat aggtgtacct
    19621 ggaccataaa tcgccgccac gccgcgctct tgcaggaagg cgtaatcctg cggtggaatg
    19681 acgccaccgg cgactacgca gatatcttcg cgtccccatt ttttcagcgc ttcgaccagt
    19741 tccgggatca gcgttttatg accggcagcc agtgaggatg cgcccactac gtgaacgtca
    19801 ttttctactg ccaggcgggc gatctcttca ggtgtagaga acatcgggct taaatctacg
    19861 tcgaaaccga gatcggaata ggcgctggcg atcactttcg cgccgcgatc gtgcccatcc
    19921 tggcccattt tggcgatcag aatgcgcggg cgacgaccat tgtcggcaag gaactgctcc
    19981 gtttgcgcaa caatggcatc gaactcggag gccgatttct cagattgatg atagctttgc
    20041 gcaatcacgc cggtaacaca ctggcttggc accagataac ggtcgaaagc ggcttccagc
    20101 gcatcggaaa tttcacccag ggtggcgcga acgcgagcgg cattaacagc ggcagccagc
    20161 aggttttcgt tatgctgtgc ggcgtgagtc agggcgttca acgcggcggt tacggcggca
    20221 tcatcacggg tggcgcgaat gcgttccagc gaagcaattt gctcgttacg caccatcacg
    20281 ttgtcgatct caagtacatc ggtttcgtct tcgtgatcca gtttgtactt gttgacacca
    20341 acgatgacac gcttgccctg gtcgatcagc gactgttcgc gcgctgaggc ctcttcgatc
    20401 attcgttttg gcagacctgc ttcgatcgct ttcgccatgc caccggcttc gtcgatctgt
    20461 tggataatag ctctggcttg tttgacgatt tgatcggtca gcgactcaat gtaataggat
    20521 ccggccagtg gatcgacggt gcggcagagt tctgattctt cctggatgat gatctgggtg
    20581 ttgcgggcaa tgcgtgctga gaaatcggta ggcaaaccaa gcgcttcgtc aaaggcgttg
    20641 gtatgcagtg actgagtacc gcccagcgtc gcagccagtg cttcaatggt ggtgcggata
    20701 acgttgttat acggatcctg ttcagtcagg ctccagcctg aggtctggca gtgggtacgc
    20761 agcgccagtg atttcgggtc ctgtgcgcca aatccactga ccgcttcgct ccataaataa
    20821 cgtgccgcac gcaacatggc gacgttcata aacagatcca tgccgatgcc gaagaagaac
    20881 gacaggcgag gagcgaagtc atcaattttc agtccggcag agattgctgc tttgatgtac
    20941 tcaatcccat cagcgagcgt aaatgctacc tgctgcacgc agttggcacc cgcttcaccc
    21001 atgtggtaac cgctgatact gatggtatta aatcgcggca tgttgccgga acaccaggcg
    21061 atgatgtcgg cgataatgcg cattgacggt tttggcgggt aaatataggt gttgcggcag
    21121 aggtactctt tgaggatatc gttttgaatg gtgccggtca gtttatcagg tgtaacacct
    21181 tgctcttccg cggcgacgat ataaaacgcc agtactggta gcactgcgcc attcatggtc
    21241 atcgaaaccg acattttatc cagcgggatc tggtcgaaca ggactttcat atcttccacg
    21301 gtgtcgatag cgacgcccgc tttgccgacg tcgcccgcca cgcgcgggtt atcggagtcg
    21361 tagcctcggt gggtggcaag gtcaaacgca acggaaagac ctttttgccc ggcggccagg
    21421 ttacggcgat aaaaagcgtt ggactctttt gctgttgaaa aaccagcata ctgacggatg
    21481 gtccacggtt gggcggtata catagtggca cgcgggccac gaacgtaggg cggcaaacca
    21541 ggaagggtac ctgtcacctc cagattatcg agatcggctt cggtatacag cggcttgatg
    21601 gcgatccctt ccgcggtttg ctgaaccagc gagtcgacag ttttctcccg acggctcaat
    21661 tccttgttgg caagctgttg ccactcctgc acgttagaca ttatttgctc cgtaaaacgc
    21721 aatcaaaatg agggtatttg cgcctacggt gagcttttta cgtgggtttg tcgccagcaa
    21781 aaaaaatgca ttcagacgtc gttttttgcc gcctgagatt tgtctatttt gtgatgtgat
    21841 tcaaggaata caaaaagaag tgaggacaac gcctgatatg tgctatcagg cattatttga
    21901 tggattaatc ctgacgaagg actttgtgac cataatcgag taacgcatca gtgactttac
    21961 gcatcatgcg gctttcagga gcaaagcggt gccagtagag catccgtcgt tgaaatagcc
    22021 caggcgttaa gtcaatcagt tcaccgctgg ccagctcttt ctcgatttgc aggtgcggga
    22081 tcatacagca ggtggtgccc tggcgagcaa gttgcacgaa tgcttctgaa gaattaacga
    22141 tatggcaggg tacgctgcca ggaggcagat cgaagttttg ctgcaaaaag gcctggtgca
    22201 tatcgtcaag atggtcaaac gcgaccactg gcgctttcag taatgccgaa cgcgttacgc
    22261 cgttagggaa atatttttcg gcaaagggtt ttgagctgac gaacagatag tcgagcgcac
    22321 caagtttatc gacaagacaa ctcggcagcg cctgatgttg aatactcacc gcgccgacca
    22381 cttcgccgcg gcgcagacgt tcctgagtgc gggtttcatc ttctacctgc aagttgaggc
    22441 ggataggcga atcagccaac acaggagcca gtgcaggaag caaccacgtc gccagactgt
    22501 cggcgttgac cgccagtgaa agcagcagcg gagtcgaacc ggtttgttca tcgcccagcc
    22561 actcttcttc cagcaactcc acctggcgca gcagtgccag cagtttttgc ccttgttccg
    22621 tcgggcgcgg cggtacggta cgcaccaaca gcggctgccc gaacatattt tccagttgct
    22681 taatgcgctg tgagacggct gattgtgtaa tgcacagctt ttgtgccgcg cgctcaaatc
    22741 ctcgttcacg tatcaccgca tccagtgcct gtaatgttct gtagtccggg cgtttcattg
    22801 ctgttgtctg ctcctggtaa atgctttttc ctgcactatg acacaatttt gtgtcaggtt
    22861 gcatataccg aagccgtcgg cgggtaacat ttgtattcgt ggttaaacgc gcagcttaaa
    22921 agatcaggga tttgcgctaa tgcatttcgc tcagcgagtt cgcgccctgg ttgtgttgaa
    22981 tggcgtggcg ttattgcctc aatttgcctg taaacagggg cttgcgaacg gtgaattggt
    23041 gcgcctgttt gcaccgtgga gcggcatacc cagaccattg tatgctttat ttgcggggcg
    23101 aaaggggatg cccgccattg cgcgatattt tatggatgag ttaaccacgc ggcttgccaa
    23161 cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa atttcatacc acaggcgaaa
    23221 cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg ggcggcactt cagtatgttc
    23281 agcccggcac cattgttggt gtaggtacag gttccaccgc cgcacacttt attgacgcgc
    23341 tcggtacaat gaaaggccag attgaagggg ccgtttccag ttcagatgct tccactgaaa
    23401 aactgaaaag cctcggcatt cacgtttttg atctcaacga agtcgacagc cttggcatct
    23461 acgttgacgg cgcagatgaa atcaacggcc acatgcaaat gatcaaaggc ggcggcgcgg
    23521 cgctgacccg tgaaaaaatc attgcttcgg ttgcggaaaa atttatctgt attgcagacg
    23581 cttccaagca ggttgatatt ctgggcaaat tcccgctgcc agtagaagtt atcccgatgg
    23641 cacgtagtgc agtggcgcgc cagctggtga aactgggcgg tcgtccggaa taccgtcagg
    23701 gcgtggtgac cgataatggc aacgtgatcc tcgacgtcca cggcatggaa atccttgacc
    23761 cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt ggtgactgtt ggcttgtttg
    23821 ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga cggtgtcaaa accattgtga
    23881 aatgatctga cgggggaacc tcccccgtta aaaaattctc ttcattaaat ttggtgacat
    23941 gtgtcacgtt tttacctggc tattgtcgat tgctctaaat aaatcctcta aaccagcata
    24001 ttcatccaag aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat
    24061 tgccgcaata ttattttttg atatgttgaa aggcggacgc aaatccgcac acaaaatttc
    24121 aaaagacagg attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct
    24181 gctggtagaa ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa
    24241 catcgaattt cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc
    24301 ccacttcatc ggcctgcgat cccgtaccca tctgactgaa gacgtgatca acgccgcaga
    24361 aaagctggtc gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc
    24421 ggcaaagcgc gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga
    24481 gctggtgatt ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc
    24541 gcaccgtggc gtgtggaaca aactggcggc ggggtctttt gaagcgcgcg gcaaaaagct
    24601 gggtatcatc ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg
    24661 aatgtatgtt tacttttatg atattgaaaa caaactgccg ctaggcaacg ccactcaggt
    24721 acagcatctt tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa
    24781 tccgtccacc aaaaatatga tgggcgcgaa agaaatttca ctaatgaagc ccggctcgct
    24841 gctgattaat gcttcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc
    24901 gagcaaacat ttggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag
    24961 cgatccattt acctctccgc tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg
    25021 cggttcgact caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa
    25081 gtactctgac aacggctcca cactttcggc tgtgaacttc ccggaagtct cactaccact
    25141 gcacggtggg cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct
    25201 gaacaaaatc tttgccgagc agggcgtcaa catcgccgcg caatatctac aaacttccgc
    25261 ccagatgggt tatgtggtta ttgatattga agccgacgaa gacgttgccg aaaaagcgct
    25321 gcaggcaatg aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt
    25381 ctctgaaaat caacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg
    25441 ttgccgcgtt gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc
    25501 aatgcgttgt gtttatttaa tggggattta cgatggcagg acagcacagg gctgtaacgg
    25561 taaagccctc accgaagcga gggcttgaag gagaagggtt atgatgcgac ttgtcatcat
    25621 actgattgta ctgttactca taagtttccc agcttattaa cagtcagtct caggggagga
    25681 gtcatcctcc cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg
    25741 gcgatgcggg atgtatcgcc ctgaaccgat catttgatag acacttcatc gcccacgttt
    25801 actgccgtaa ttaatccctt atccctgcaa tgtgtgtctg tcttacatga cgttttacga
    25861 gtgcgctcga agaaaaaatt taaaggtaga tcaatgcgtt gtgcttattt aaagagagtt
    25921 tacgatagca gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttga
    25981 aggagaaggg ttatgatgcg acttgtcatc atactgattg tactgttact cataagtttc
    26041 agcgcttatt aacagtcagt cacaggggag gagcaatcct cccttaccct tactcactaa
    26101 attaggttaa agaatcaacg atgtcaatca gggcgatgca ggtgtgtcgc cctcaccact
    26161 cccagatttt cgacggtgta accaccgcag gaagagggat atcccactct tcaacgggga
    26221 gtttttccac caactgacaa tcatgcgcat aacccaccgg ttgcatttta tagtgctgcc
    26281 agttttgtaa ggtccgatca taaaaacctc cgcccattcc caggcgctga ccgtactcat
    26341 caaaggcgac cagcggtgtg atcaacacgt ctaatcggga aaggggtagc acgtcacgca
    26401 catccagttt tggctcatgg atcttcaacc tgttcatcac cagttcactt tgcggatggt
    26461 aattcaggaa cagcaaatta ccggcactaa agggatgcaa aactggaaga tatacgcgct
    26521 taccggcgcg ccagagttgt tctataagcg gctgggtgtc gagttctcca tcaaaagaga
    26581 ggaatacagc gaccgtatgt gccatcacca ccgggggata agtcatcatc cgggtagcgg
    26641 cttgttgacc catttcctgc tgttgttccg gcgttaacgc acgacgacgt tgccgaatca
    26701 ttttgcggat ttcttgtcgg gataatgtca gtgggagttc tggtagttgc gtcatggctg
    26761 gtgccagata agaagggaat ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg
    26821 gttcaaggtg aatgtgtcgt cgcagtttta aggcttctcg gacggaccga gcatgctcac
    26881 caaccgcgga gcgccacatt cttgtggtat gaaatatcgg ctcaggggac tggcccgctt
    26941 gcgaacatct cagagaaatt ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg
    27001 tcattcaaag ttttggttag ttttttcggt gatgcgacct tgttcaagta acgcttgttc
    27061 tatggtctgc tgcagcatcc gaatacgctg ttccatactt gccgcgtagt cacgagtctt
    27121 tgctttttct tgcgctaact catagctgat attcaatgcg gcaatgaaga ccaactgttc
    27181 agtatttgtg actctagtgc gttctttcag atcttgcaac cgttggttca gatcgtccgc
    27241 tgcctgattc aacgcatccc tttggtcagg cgggcagttc acacgcagtg aacggccaaa
    27301 aatttggata tcgacgggtt gtgcagacat gccaccttcc tgctgattga ctgcgctgcc
    27361 ttcgtcttta gacccatggt ctgcgaaggg gcgacactat agctaccctg atgagaagag
    27421 acaagccctt ttctggtcca ccagggccca aagtggtagc atatcatgaa tattcctccc
    27481 tttgacgacg aatgcttatg tctatacaga acgaaatgcc tggttacaac gaaatgaacc
    27541 agtatctgaa ccaacaaggg acgggtctga cccctgctga gatgcatggt ttaatcagtg
    27601 ggatgatatg tggcggtaac gatgacagct catggctgcc gctacttcac gacctgacga
    27661 acgaaggcat ggctttcggt catgagctgg cacaggcact gcgtaaaatg cactctgcca
    27721 ccagcgatgc cctgcaggat gacggcttcc tttttcagct ttatctgcct gatggcgatg
    27781 atgtcagcgt tttcgatcgg gctgatgcac tggcaggttg ggtcaatcac ttcctgcttg
    27841 gtcttggcgt tacgcaaccg aagctggaca aagtgaccgg agaaaccggt gaagccatcg
    27901 acgatctgcg taacatcgcg cagctgggct acgacgaaga cgaagatcag gaagagcttg
    27961 aaatgtcgct tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca
    28021 cctttactca tccgcaaccg accgcgccag aagtacaaaa accgactcta cactaaaaac
    28081 aaaaaacgta aggagagtgt tatgagtgag atatcccggc aagagtttca gcgtcgccgt
    28141 caggccctgg tggagcaaat gcaacccggc agcgctgcgc tgatttttgc tgcaccagaa
    28201 gtaacacgta gcgccgacag cgaatacccc tatcgtcaga acagtgactt ctggtacttc
    28261 accggcttta acgaaccgga agcggtgctg gtgctgatta aaagcgatga cactcataac
    28321 cacagcgttc tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt tggccgtcgt
    28381 ttaggtcagg atgccgcgcc agagaaactg ggcgttgacc gcgcactggc attcagcgaa
    28441 attaatcagc aactttatca actacttaat ggtctggatg tggtttacca cgcccagggc
    28501 gaatatgcat atgctgatga aatcgtgaac agcgcgcttg agaaactgcg taaaggttcg
    28561 cggcaaaatc tcaccgcgcc ggcaacgatg atcgactggc gtcccattgt tcatgaaatg
    28621 cgcctgttca aatcaccaga agagattgcc gtacttcgcc gcgcggggga aatcaccgcc
    28681 ctggcacata cacgggcgat ggaaaaatgc cgtcctggga tgttcgagta ccatctggaa
    28741 ggcgaaattc accacgaatt taaccgccac ggcgcgcgct atccttccta taacactatt
    28801 gtcggcagcg gtgaaaacgg ctgcattctg cactacaccg aaaacgagtg tgaactgcgt
    28861 gacggagatc tggtgttgat tgatgcgggt tgcgaataca aaggttacgc tggcgatatt
    28921 acccgcacct tcccggtcaa cggcaaattc actcaggctc agcgtgaaat ctacgacatt
    28981 gtgctggagt ctctcgaaac tagcctgcgc ctgtatcgtc cgggaacttc cattcaggaa
    29041 gtcaccggtg aagtggtgcg catcatggtt agcggcctgg taaaactcgg catcctgaaa
    29101 ggtgatgttg atgaactgat cgctcagaac gcccatcgtc ctttctttat gcatggcctt
    29161 agccactggt taggactgga tgtccatgac gttggcgttt atggccagga tcgctcgcgc
    29221 attctggaac cgggcatggt actgaccgta gagccagggc tgtatattgc gccggatgca
    29281 gaagtgccag aacaatatcg cggtatcggc attcgtattg aagacgacat tgtgattacc
    29341 gaaaccggta acgaaaacct caccgccagc gtggtgaaaa agccggaaga aatcgaagcg
    29401 ttgatggcag ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc atggcgggcg
    29461 cgacgctggc gctggctatt tcccggttaa gtcacggggc gctgccggta catttgattg
    29521 aagcgaccgc gccagagtca catgctcatc cgggctttga tgggcgagcg attgcgctgg
    29581 cggcgggtac ctgtcagcaa ctggcgcgca ttggcgtctg gcaatctctg gcggattgcg
    29641 caaccgccat cactaccgtg catgtcagcg atcgtggtca tgccggattc gtcaccctcg
    29701 ccgcagaaga ttaccaactg gcggcgctgg ggcaggttgt cgaattgcac aatgtcgggc
    29761 aacggctgtt tgcattgctg cgcaaagcac ctggcgtaac gctgcattgc ccggatcgcg
    29821 tggctaacgt tgcccgtact cagagtcatg ttgaagtgac gctgcagtgt ggcgagacgc
    29881 tgacgggccg cgtgctggta gccgctgatg gcacccattc agcgttagcc actgtgtgcg
    29941 gcgttgactg gcagcaggag ccttacgaac aactggctgt gattgccaac gttgcgactt
    30001 ccgttgcgca tgaagggcgc gcttttgaac gctttacgca acatggcccg ctggcgatgt
    30061 tgccgatgtc tgacggacgc tgttcgctgg tctggtgtca tccactggaa cggcgcgaag
    30121 aggtgctgtc gtggagcgac gagaagtttt gccgtgaact ccagtcggcc tttggctggc
    30181 gacttgggaa aattacccac gctggtaaac gcagtgctta tccgctggcg ttaacccacg
    30241 ccgccagatc tattacccat cgtaccgtgc tggtgggcaa tgcggcgcaa accctgcacc
    30301 cgattgccgg gcaagggttc aacctcggta tgcgagatgt catgagtctt gcggaaaccc
    30361 tgactcaggc gcaggagcgc ggagaagaca tgggtgatta cggcgtattg tgtcgttatc
    30421 agcagcgtcg acagagcgat cgcgaagcaa ccattggcgt cacggacagc cttgtacatc
    30481 tttttgccaa ccgttggacg ccactggttg tcgggcgcaa catcgggctg atgacgatgg
    30541 aattattcac cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg gtggcgcgtt
    30601 gaaggcggtt ttaaggaata aacaatgcaa agtgttgatg tagccattgt tggcggcggc
    30661 atggtggggc tggcggttgc ctgcggctta caggggagcg gcttacgcgt tgccgtactg
    30721 gagcagcgcg taccggaacc tctggcggcg gatgcaccac cacaactgcg cgtttcggct
    30781 atcaatgccg ccagcgaaaa attactcacc tgtcttggcg tctggcagga cattctctct
    30841 cgtagggcca gttgctatca cggcatggaa gtgtgggaca aagacagttt tggtcacatc
    30901 tcgtttgacg atcaaagcat gggctatagc catcttgggc atatcattga aaactcagtg
    30961 attcactacg cgctgtggaa caaagcgcat cagtcgtcag atatcactct gttggccccc
    31021 gcagaattac agcaggtcgc ctggggagaa aacgaaacct tcctgacgct gaaagatggc
    31081 agcatgttaa cggcgcgtct ggtgattggt gcggatggcg ctaattcctg gttgcgcaac
    31141 aaagccgata ttccgctgac tttctgggat tatcagcatc acgcgctggt agcgaccata
    31201 cgcacggaag aaccgcatga tgcggtggcg cggcaggttt tccatggcga aggcattctg
    31261 gcctttttac cgcttagcga tccgcatctt tgctcgattg tctggtcact gtcgccagag
    31321 gaagcgcagc ggatgctgca ggcaagtgaa gacgaattta atcgcgcgtt aaatatcgct
    31381 tttgataatc gcctgggctt atgcaaggtt gagagcgagc gtcaggtgtt cccgctgaag
    31441 gggcgttatg cgcgccagtt tgccgcacac cgtctggcgc tggtgggcga cgccgcgcat
    31501 accattcacc cactggcggg gcagggcgtt aatctcggct ttatggacgc cgcagagctg
    31561 attgctgaac tgaaacggtt gcatcgtcag ggtaaagaca tcgggcagta catttacctg
    31621 cgccgctatg aacgtagccg caagcacagc gcggcgctga tgctggctgg tatgcagggt
    31681 tttcgcgatc tcttttccgg cgctaatccg gcgaaaaaac tgctgcgcga tattggtctt
    31741 aaactggctg atacactccc tggcgtgaag ccgcagctta ttcgtcaggc aatgggatta
    31801 aacgatttgc ctgagtggct gcgttaaaaa tttctcttct gctgtttatt tgatacgcat
    31861 cacactttca tctcccggtt ttttcgccgg gagattttcc tcatttgaaa taaactaatt
    31921 tcacctccgt tttcgcatta tattttctaa tgccatgatt ttttgattta gtgttttttg
    31981 acattttttt agctcttaat attgtcttat tcaaattgac tttctcatca catcatcttt
    32041 gtatagaaac tggtgtgttt tttggttttt tattctgtcg cgatttttgc attttttaac
    32101 cataagctaa tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt
    32161 tcaggcaaaa gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggttttgtg
    32221 gtgataattt caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca
    32281 caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac
    32341 ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc
    32401 gatgccggaa tgtttgatgt gtcacatatg accatcgtcg atcttcgcgg cagccgcacc
    32461 cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa
    32521 gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgatct catcgtctac
    32581 tactttactg aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc
    32641 tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt
    32701 tccatgattg ctgtacaagg gccgaatgcg caggcaaaag ctgccacact gtttaatgac
    32761 gcccagcgtc aggcagtgga agggatgaaa ccgttctttg gtgtgcaggc gggcgatctg
    32821 tttattgcca ctaccggtta caccggtgaa gcgggctacg aaattgcgct gcccaatgaa
    32881 aaagcggcgg atttctggcg cgcgctggtg gaagcgggcg ttaagccatg cggcctgggc
    32941 gcgcgtgaca cgctgcgtct ggaagctggc atgaatttgt atagtcagga gatggacgaa
    33001 accatctctc ctttagccgc caacatgggc tggaccatcg cctgggaacc ggcagatcgt
    33061 gactttatcg gtcgtgaagc tctggaagcg cagcgtgaac atggcacaga aaaactggtt
    33121 ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ttttaccgat
    33181 gcgcagggca accagcatga aggcattatc accagcggga ctttctcccc gacgctgggt
    33241 tacagcattg cgctggcgcg cgtgccggaa ggtattggtg aaacagcgat tgtgcaaatt
    33301 cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa cggcaaagcc
    33361 gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat
    33421 acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttatacc gttggcatca
    33481 ccgaacacgc tcaggagttg ttgggcgata tggtgtttgt tgacctgccg gaagtgggcg
    33541 cgacggttag cgcgggcgat gactgcgcgg ttgccgaatc ggtaaaagcg gcgtcagaca
    33601 tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgtactgagc gattccccgg
    33661 aactggtgaa cagcgaaccg tatgcaggcg gctggatttt caaaatcaaa gccagcgatg
    33721 aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagacgagt
    33781 aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaatttac gcctcaccct
    33841 cactctcttc gtaaggagag aggttcacaa ttcactgcac gtttcaggaa ccatcgctca
    33901 tgacacagac gttaagccag cttgaaaata gcggcgcttt tattgaacgc catatcggac
    33961 cggacgccgc gcaacagcaa gaaatgctga atgccgttgg cgcacaatcg ttaaacgcgc
    34021 tgaccggcca gattgtaccg aaagatattc agcttgcgac cccgccgcag gttggcgcac
    34081 cggcgaccga atacgccgcg ctggcggaac tcaaggctat tgccagtcgc aataaacgct
    34141 tcacgtctta catcggcatg ggttacaccg ccgtgcagtt accgccggtt atcctgcgta
    34201 acatgctgga aaatccgggc tggtataccg cgtatactcc gtatcaacct gaagtctcgc
    34261 agggccgcct tgaggcactg ctcaatttcc agcaggtaac gctggattta accgggctgg
    34321 atatggcctc tgcttctctt cttgacgaag ccaccgctgc cgccgaagca atggcgatgg
    34381 cgaaacgcgt cagcaaactg aaaaatgcca accgcttctt cgtggcttcc gatgtgcatc
    34441 cgcaaacgct ggatgtggtc cgtactcgtg ccgaaacctt tggttttgaa gtgattgtcg
    34501 atgatgcgca aaaagtgctc gaccatcagg acgtcttcgg cgtgctgtta cagcaggttg
    34561 gcactaccgg tgaaattcac gactacactg cgcttatcag cgaactgaaa tcacgcaaaa
    34621 ttgtggtcag cgttgccgct gatattatgg cgctggtgct gttaactgcg ccgggtaaac
    34681 agggcgcgga tattgttttt ggttcggcgc aacgctttgg cgtaccgatg ggttacggtg
    34741 gcccacacgc ggcattcttt gcggcgaaag acgaatacaa acgctcaatg ccaggccgta
    34801 ttatcggtgt atcgaaagat gcagctggca ataccgcgct gcgcatggcg atgcagactc
    34861 gcgagcaaca tattcgtcgt gagaaagcga actccaacat ttgtacttcc caggtgctgc
    34921 tggcaaacat cgccagcctg tatgccgttt atcacggccc gattggcctg aaacgtatcg
    34981 ctaaccgcat tcaccgtctg accgatatcc tggcgacggg cctgcaacaa aaaggtctga
    35041 agctgcgcca tgcgcactat ttcgacactc tgtgtgtgga agtggccgac aaagcgggcg
    35101 tcttggcgcg tgccgaagcg gctgaaatca acctgcgtag cgatattctg aacgcggtgg
    35161 ggatcaccct tgatgaaacc accacgcgtg aaaacgtgat gcagcttttc agcgtgctgc
    35221 ttggcgataa ccacgggctg gagatcgaca cgctggacaa agacgtggct cacgacagcc
    35281 gctctatcca gcctgcgatg ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc
    35341 gctaccacag cgaaaccgaa atgatgcgct atatgcactc gctggagcgt aaagatctgg
    35401 cgctgaatca ggcgatgatc ccgctgggtt cctgcaccat gaaactgaac gccgccgccg
    35461 agatgatccc aatcacctgg ccggaatttg ccgaactgca tccgttctgc ccgccggagc
    35521 aggccgaagg ttatcagcag atgattgcgc agctggctga ctggctggtg aaactgaccg
    35581 gttacgacgc cgtttgtatg cagccgaact ctggcgcaca gggcgaatac gcgggcctgt
    35641 tggcgattcg tcattatcat gaaagccgca acgaagggca tcgcgatatc tgcctgatcc
    35701 cggcttctgc gcacggcact aaccctgctt ctgcacatat ggcaggaatg caggtggtgg
    35761 ttgtggcatg tgataaaaac ggcaacatcg atctggctga tctgcgcgcg aaagcggaac
    35821 aggcgggcga taacctctcc tgtatcatgg tgacctatcc ttccacccac ggcgtgtatg
    35881 aagaaacgat ccgtgaagtg tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg
    35941 atggcgcgaa catgaacgcc caggttggca tcacctcgcc gggctttatt ggtgcggacg
    36001 tttcacacct taacctacat aaaactttct gcattccgca cggcggtggt gggccgggta
    36061 tgggaccgat cggtgtgaaa gcgcatctgg ctccgtttgt accgggtcat agcgtggtgc
    36121 aaatcgaagg catgttaacc cgtcagggcg cggtttctgc ggcaccgttc ggtagcgcct
    36181 ctatcctgcc aatcagctgg atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag
    36241 caagccaggt ggcaatcctc aacgccaact atattgccag ccgcctgcag gatgccttcc
    36301 cggtgctgta taccggtcgc gacggtcgcg tggcgcacga atgtattctc gatattcgcc
    36361 cgctgaaaga agaaaccggc atcagcgagc tggatattgc caagcgcctg atcgactacg
    36421 gtttccacgc gccgacgatg tcgttcccgg tggcgggtac gctgatggtt gaaccgactg
    36481 aatctgaaag caaagtggaa ctggatcgct ttatcgacgc gatgctggct atccgcgcag
    36541 aaattgacca ggtgaaagcc ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc
    36601 cgcacattca gagcgaactg gtcgccgagt gggcgcatcc gtacagccgt gaagttgcgg
    36661 tattcccggc aggcgtggca gacaaatact ggccgacggt gaaacgtctg gatgatgttt
    36721 acggcgaccg taacctgttc tgctcctgcg taccgattag cgaataccag taattcactg
    36781 attcgactat cttctaaagg cgcttcggcg cctttttagc ttgttaataa agtgcgcttt
    36841 gtgtgtcgga tgaagcgtaa atataaaatc ctggagttca atatatgaca ggagcgccag
    36901 gactgatgag cggggcgcat caggcacatc gggtaattaa agcttctcgc cgttgctggc
    36961 aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat cgccagtacc
    37021 gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc cggtggtgaa
    37081 cgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacgc catcgtaagt
    37141 catcgctttt ttcatctctt cgatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt
    37201 gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga caataaacag
    37261 cggcttctgg taacgctcat acagttcgca aagggcatag cgcagaccta ctggatcaat
    37321 ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt cgaaaccaga
    37381 gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg gtcatgtaat agctgaaacc
    37441 aagataatcg caggtgcctt tgcgcagtac atccagatcg ccgtcttcca ttttgatgtt
    37501 aaatccgcga cgctcccact cgttcaatac ataggacggg taatagccgc gtagctgcac
    37561 atcggtaaag acgtaacgtt cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt
    37621 acaggagtaa ggatagagcg gcaccatcgc cagcatacag ccgactttca tctccgggtt
    37681 aatgcgacgc gcagctttca ccgccagggc gctggcgaca aactggtgat gcagcacctg
    37741 atacatcgtc tcttccgggt tctcatgctc ggtataaacc acgccggagc agcagtaacc
    37801 gaacagcggt gcacgccagt tacgctggtt gttaatttcg ttgaaggtca tccagtattt
    37861 gactttatgc ttatagcgtt caaataccac ttccgcgaaa cggacaaaga aatcaaccac
    37921 tttacggttg gtccagctac cgtattgctg caccagatgc agcggcattt caaagtggga
    37981 gagggtgatc actggttcga tgttgtattt cagcaattca tcgaacagag agtcgtaaaa
    38041 cttcagccct tcttcgtttg gctgagcttc atcgcctttt ggaaaaatgc gcgtccaggc
    38101 gatggatgta cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt cttccttgta
    38161 gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca agacttcttt
    38221 ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa tgctcggccc
    38281 tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc cgccccataa
    38341 gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatgag tcaattctta
    38401 caggaaatag taccatgcag ccaaacgaca tcactttttt tcaacgtttc caggatgaca
    38461 ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg
    38521 gtgatgtgct tcgtgtcggg cgttttgaag atgacggtta tttttgcacg attgaagtca
    38581 ccgcaacctc aaccgtaacg ctggatacgc tgacggaaaa acatgcagag caggaaaata
    38641 tgaccctgac tgaactgaaa aaggtcattg ccgacatcta tcccggtcag acacagtttt
    38701 atgtgattga atttaaatgt ctttaaattg aactcacgcc tgttagttga aatttcgcat
    38761 ttaatttcat gatttttagg ggattagtct caaatcagaa tttattttag ctaacaggtg
    38821 ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta cgtttctccg
    38881 gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc agaggaaatt
    38941 gccaacagcg tcagtcacgg cattgggttg gtgtttggta tcgttgggct ggtgttgcta
    39001 ctggttcagg cggtggatct caatgccagc gccacagcga taaccagcta cagcctctat
    39061 ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat tcctcatcaa
    39121 cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt gattgccgga
    39181 acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg gttgatgatt
    39241 gttatctgga gcctggcatt gctggggatt ctgttcaaac tgaccatcgc gcaccgattc
    39301 aaaattttat ctctggtgac ttatctggcg atgggctggc tatcgctggt ggtaatttat
    39361 gaaatggcag ttaagctcgc ggcgggcagc gttaccttac tggcggtagg cggcctggtt
    39421 tattcgctcg gggtgatttt ctacgtctgc aaacgcattc catacaacca tgccatctgg
    39481 cacggcttcg tgctcggcgg tagtgtgtgc cactttctgg cgatctattt gtatattggg
    39541 caggcgtaat gggtatgcct ttgtaggcct gataagacgc gccagcgtcg catcaggcga
    39601 taaatgcctg atgcgacaca gagatttact cttcgagcga atacggcagc ggctcgatat
    39661 gcaatgtatt cgcatcgtca cgcacgcgga agatgctgtc cggttccata tcgttattca
    39721 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
    39781 tgcgacgcca gttctcgccc attttcagct ctaaatcttc accagcttcc ggcagtcggc
    39841 tggcactacc tgtcagcaac cagagcgcgc gtttgttggc accacggaat tttgctcgcg
    39901 ccaccatctc ttgtccggta tagcagcctt tcttaaagct gatgccaccg agcgcctgaa
    39961 gattggttgc ttgcgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
    40021 cttcaatgtt taatgccagc cactgttggc tattgttcaa ctccgcttca ccgcgcagtt
    40081 tatcggtcaa tgtattggcg gtagctgcat cggttacgat cagaaaacgt tccgccggat
    40141 gttcaaacca tagcagagta gtcgcacctt ctttaactac ctgtttttct ttcgaaggca
    40201 gttcgctaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccgagcagca
    40261 cacgctcgtc gtctggcgcg atggtcactt tagagaacac cgcatatttt ttcagttcag
    40321 tcagctgcga atcacgcacg ctgcggcgtt caatccacgc aaagccatcg ccgtcgcgga
    40381 acagacgcaa attgctccac attttacctt tggcgtcgca atgagcggcg agcaaatgct
    40441 ggtcttctga catctgacag acatctgctg ttacctggcc ctgcatatat ttttcgctgt
    40501 ccgcgccaga aatggtagca agcgcccagt catcaagcgt catcagcgtc agtggtaaac
    40561 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
    40621 ttgcttaacg gcgtattaat aactaatggt aaaagagcta tgaggcaatg caagtgcttt
    40681 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc cttgaattgt
    40741 tgtgaattaa tttcgcgatc gcgattgtgt ttactgatat tcccgtaaaa gaagtcgaaa
    40801 aacacgttac aatagcgggg tactggattt tcgtgagaca ggaagaagat ggacattaac
    40861 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
    40921 atgccgtttt tcgaacatga gtacgacaga ttaagcgatg acgaaaaacg catctttatt
    40981 cgtctgcttg aatgtgacga tccagacctg tttaactggc tgatgaatca cggtaaaccg
    41041 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
    41101 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcgcag tggctttcct tgctgattca
    41161 tgggctggtt gccgctgtta ttttactcat gccctggccg ctcagttaca ccccgttatg
    41221 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gcattaatgc
    41281 tcgccagggg gaaattcgcc tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
    41341 gagcatcgtc aaagcgccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
    41401 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
    41461 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
    41521 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
    41581 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
    41641 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
    41701 ccgttggcca gtagccgacg aactttacgc ctttggtcga gagtttgtca tgcagcatac
    41761 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
    41821 caattttacc ttcgaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct
    41881 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
    41941 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
    42001 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
    42061 tcataacgta agcttctcga ttctgctgtg gcttttgcgg ctagtgtatc agaatcgctt
    42121 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
    42181 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
    42241 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
    42301 ccgcgggttg acgctggcga cggcgcaatg tgacgatttg caggcattac tggcagaacg
    42361 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
    42421 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
    42481 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggttg aacgtttatt
    42541 acaggctcca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
    42601 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
    42661 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg tgccgttagg
    42721 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
    42781 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
    42841 cttctggcac cgtattaaac attatgccgt gctggcgggt atcgacagcg aaaagctgtc
    42901 accgcatgtg ttgcgtcacg cttttgccac tcatttatta aatcatggtg cggatttacg
    42961 cgtggtgcag atgctactgg gccacagcga tctctccacc acgcaaattt atacgcatgt
    43021 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
    43081 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
    43141 aggctttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
    43201 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
    43261 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
    43321 tggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
    43381 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
    43441 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactaca acgcgctggg
    43501 gatcaccgtg cgttatcttg ctttcccgcg ccaagggctg gatagcgatg ctgagaaaga
    43561 aatgaaagct atctggtgtg cgaaagacaa aaacaaagcg tttgatgatg tgatggcagg
    43621 taaaagcgtc gcaccagcca gttgcgacgt ggatattgcc gaccattacg cacttggcgt
    43681 ccagcttggc gttagcggta ctccggcaat tgtgctgagc aatggcacac ttgttccggg
    43741 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
    43801 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
    43861 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg aggagtacgc
    43921 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggc
    43981 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
    44041 gtcggtgatt tcgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
    44101 tcgcttggtt gcagcaatat cgactacctg gtaccaaacc gtttcgaaga cggttacggc
    44161 ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
    44221 gataacggta tttcctccca tgcgggggtt gagcacgctc gctcgttggg catcccggtt
    44281 attgttaccg atcaccattt gccaggcgac acattacccg cagcggaagc gatcattaac
    44341 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
    44401 tatctgatgc tggcgctgcg cacctttttg cgtgatcagg gctggtttga tgagcgtggc
    44461 atcgcaattc ctaacctggc agaactgctg gatctggtcg cgctgggaac agtggcggac
    44521 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
    44581 gccggaaagt gccgtccagg gattaaagcg ctgctggaag tggcaaaccg tgatgcacaa
    44641 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccgggcga
    44701 ctggacgata tgtccgtcgg tgtggcgctg ttgctgtgcg acaacatcgg cgaagcgcgc
    44761 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
    44821 caggttgaag ccctgaccct gtgcgagaaa ctggagcgca gccgcgacac gctacccggc
    44881 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
    44941 atcaaagagc gttttcatcg tccggttatc gcctttgcgc cagcaggtga cggtacgctg
    45001 aaaggttccg gtcgctccat tcaggggctg catatgcgtg atgcgctgga gcgattagac
    45061 acactctacc ctggcatgat gctgaagttt ggcggtcatg cgatggcggc gggtttgtcg
    45121 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tactgagtgg
    45181 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag tccagccgaa
    45241 atgaccatgg aagtggcgca gctgctgcgc gatgctggcc cgtgggggca gatgttcccg
    45301 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
    45361 ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat
    45421 gtcgataccg ccctctggcc ggataacggc gtgcgtgaag tgcaactggc ttataagctc
    45481 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
    45541 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
    45601 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
    45661 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
    45721 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
    45781 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
    45841 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
    45901 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
    45961 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
    46021 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
    46081 tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga
    46141 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
    46201 ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaaa gcccgtttga
    46261 ctccggcggt cgtcgccaca cgtcgttcag ctccgcgttt gtttatccgg aagttgatga
    46321 tgatattgat atcgaaatca acccggcgga tctgcgcatt gacgtttatc gcgcgtccgg
    46381 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattactc acatcccaac
    46441 cgggatcgtg acccagtgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
    46501 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
    46561 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
    46621 tgatgactcc cgcattaaag atctgcgcac cggggtagaa acccgcaaca cgcaggccgt
    46681 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
    46741 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
    46801 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
    46861 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggca aagagaacga
    46921 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
    46981 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggg ggccgcattc agctgtacgt
    47041 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
    47101 cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
    47161 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
    47221 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
    47281 atcccgtaac acctttaaag tgcgctcgca gatcctctct ggtattcgcc agttcatggt
    47341 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgatcccag gcggtgccgc
    47401 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
    47461 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
    47521 ccgtaacttc cgtaacgaag gtatctccgt acgtcataac ccagagttca ccatgatgga
    47581 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
    47641 tactctggca caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga
    47701 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
    47761 aaccgacatg gcggatctgg acaacttcga ctctgcgaaa gcgattgctg aatctatcgg
    47821 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
    47881 ggctgaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
    47941 gctggcgcgt cgtaacgacg ttaacccgga aatcacagac cgctttgagt tcttcattgg
    48001 tggtcgtgaa atcggtaacg gctttagcga actgaatgac gcggaagatc aggcgcagcg
    48061 cttcctggat caggttgccg cgaaagatgc aggtgacgac gaagcgatgt tctacgatga
    48121 agattacgtc accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
    48181 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
    48241 ggcgatgcgt ccggtgaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
    48301 ttttttattt aagctgggta aatgcagata atcgtattct ggcttcgcga tttgtcgcct
    48361 gcatcaccat ccacggactg aacgcccacg gcgtggcatc aataccgcgt aatacatcag
    48421 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
    48481 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccattcgga tcggtggcgc
    48541 gatagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
    48601 ggcaacggcg gatcaccgcg tcttcgttgc tttctcccag ttgtgggtgc ccacaaaccg
    48661 agttagtcca cacgccaggc catgcttttt tgctcagtgc gcggcgggta actaataatt
    48721 gtcctttggc attaaacagc caactggaga acgcgagatg taagcgggtg tctgccgtgt
    48781 gtgcggcata cttttccagc gtacccgtgg gaactccctg tgcattcaat aaaatgacgt
    48841 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
    48901 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgtcg
    48961 acaggtaaaa accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
    49021 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
    49081 agcgcgcatt aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca
    49141 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
    49201 ggtcagcatg atgctggtag ccattaaaga agacattcag cacaacggcg ctaagggttg
    49261 cgagcataat gccgctatgt agcagcggtt gcagtacggc cggtaactta gaaaagaaat
    49321 cgtgagaaag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
    49381 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
    49441 cgaacatcac cagaccagcg ccgcccagca caaattgcgg aatggaggct accagcaccg
    49501 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
    49561 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg agaaaatgac gtatggggga
    49621 agctgttaaa cgttccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    49681 tatcgtgcga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    49741 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    49801 tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca cgaggcatca tgtagcccgg
    49861 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    49921 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    49981 cggcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    50041 cggcccagtc aatacccacc tgaatgatgc taagcccgat agaagtaata accacaccgg
    50101 taaccagtgg cggaaataaa ggcatcaagc gaccgataag tggcgctaat aatgtggtga
    50161 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    50221 caatggctat cattggtgtt acagcagcaa acgttaccga cataatcacc ggcaggcgga
    50281 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    50341 gatccgagct aatgagcatc gcaatagctt cttttgagag gcccagtcgg tcaccaatca
    50401 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    50461 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    50521 atgaaggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    50581 aaaagcaacc gtttacgtta atgaaactgt ctaaacacat gctcgtggag tgccagtttt
    50641 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtgcttaaac
    50701 cacgaaaatg agaagagaac ctgacaatgg aattatctgt aattttctca tattgatatt
    50761 tcattaatca ttctggcatg tcgcactctc gcatttaatc gtttttatct ggatagcgct
    50821 atttggggcg gcaatgttga aataatgaaa gcggcataga ttgaggggca ataatttgaa
    50881 ctcagtcgca aaattataat taaaacggtc atgctaccta atatatgccc gtttattgca
    50941 tcgcgaaatt tgagaagaca taactcatta aatgaaaggg atgcaaatga ataagtttat
    51001 cgctgctgaa gctgcggaat gtataggctg tcatgcttgt gaaattgcct gtgcggtggc
    51061 acacaatcaa gaaaactggc cgctgagtta cagtgacttt cgaccacgta tccacgttgt
    51121 agggaaaggc ctggctgcga atccggtggc ctgccatcac tgcaacaatg ccccttgcgt
    51181 tacggcttgt ccggttaatg ctctgacttt ccagtccgat agcgtacaac tggacgagca
    51241 aaaatgtatt ggttgtaaaa gatgcgcaat cgcttgcccc tttggcgtcg ttgagatggt
    51301 cgatacgatt gcgcagaaat gcgacctttg taaccagcgc agttccggca cgcaagcctg
    51361 tattgaagtt tgcccaacac aggcgttacg actgatggac gataaagggt tacagcagat
    51421 aaaggtggcc cgccagcgca aaacggcagc aggaaaagcg tcatcagacg ctcagccatc
    51481 tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc gcagataaaa tttcagcgag
    51541 tgaacggaaa acccactttg gcgaaatcta ttgcgggctg gatccactgc aagcgactta
    51601 tgagagtgac cgctgtgttt attgtgccga aaaagctaac tgcaactggc attgcccgct
    51661 gcataacgct attccggatt acatccgtct ggtacaggaa ggaaatatta ttgaagcggc
    51721 agaactttgc caccagacca gttccttacc agaaatctgc ggcagggtat gtccacagga
    51781 ccgtctttgt gaaggcgcat gtactttgaa agatcactct ggcgcagtct ctatcggtaa
    51841 tctggaacgc tacatcaccg ataccgcgct ggcgatgggc tggcgtcccg atgtcagcaa
    51901 agttgttccc cgtatcgaga aagtggcggt gattggcgct gggcctgcag ggttagggtg
    51961 tgctgatatt ctggcgcgcg caggagttca gatcgatgtc tttgatcgcc atccagaaat
    52021 tggcggtatg ctgacttttg gcattcctcc tttcaaactc gataaaacgg tattaagcca
    52081 gcggcgagag atattcaccg caatgggaat cgactttcat cttaactgtg aaattggccg
    52141 cgatattacc tttagcgatt taacttctga atatgatgca gttttcatcg gcgtggggac
    52201 ttacgggatg atgcgagctg atctgccgca tgaagatgcg cccggtgtca ttcaggctct
    52261 accgttcttg actgcccata cccgccagct catggggttg ccggagtctg aagagtatcc
    52321 gctgacggac gtggaaggta agcgagtcgt ggtattgggc ggtggcgata cgacaatgga
    52381 ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg acctgcgcgt atcgtcgtga
    52441 tgaagtcagt atgccgggct cgcgcaaaga ggtggtcaat gcgcgcgagg aaggtgtcga
    52501 gtttcaattc aatgttcagc cgcaatatat cgcttgtgac gaagatgggc gtttaactgc
    52561 ggtgggcctg attcgtaccg ccatgggtga gccggggccg gatggtcgcc gtcgtcctcg
    52621 tccggttgcg ggttcagagt ttgaattgcc cgccgatgtt ctcattatgg cctttggttt
    52681 ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt aaactcgata aatggggcct
    52741 gattcaaacc ggcgacgttg ggtatttacc tacccagacg catctgaaaa aagtatttgc
    52801 tggtggggat gcagttcatg gcgcggatct ggttgtcact gccatggccg caggaaggca
    52861 ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca tcgtgatgaa atcgttaatt
    52921 atcgttaatc cggctgactg tattggctgc cgtacttgtg aagtggcctg tgtggtcgct
    52981 cacccttcag aacaagagtt gaatgccgat gtctttttac cccggctgaa ggtacagcga
    53041 ctggactgca tcagtgcgcc ggtgatgtgc catcagtgtg aaaacgcccc ttgtgttggc
    53101 gcttgccccg tgggggcgct gacgatgggc gagcaggtgg tgcagaccaa ttctgcccgt
    53161 tgtattggct gtcagagttg cgtcagtgcg tgcccgtttg gaatgatcac cattcagtcg
    53221 ttgccggggg atacccggca acaaatcgtg aaatgcgatc tctgtgaaca gcgggaagag
    53281 gggcctgcct gcgttgaatc ttgcccaaca caggcgctgc agttgcttac tgaaagagaa
    53341 ctcaggcgag tccgccagca gcgtattgtt gccagcggtg agaatcctct gtgaaagaaa
    53401 tctccgcgct gaaaggcgcg gagaatctat tagattgccc aaccacccgc gtaaaatgcg
    53461 accagtgcgg cagtaatgat caccgtacca atattcagct tttgccattc gcgtgcaaag
    53521 acgcggccta cgaccagtgt cacaaagccc agcataatac cggtaacgat attacaagtc
    53581 agaacgatga acacggcaca caccaggcca gccatagcgt caataaaatc attgaaatcc
    53641 agcttcgaga cgttacttaa catcagcaaa cctacgtaca tcagagcggg tgcagtggcg
    53701 taaccaggga tcaaaaatga cagcggtgat aagaacagaa tcagcaggaa taacgccccc
    53761 actacggttg cggttaaccc tgttttaccc ccggcggcgg ttcctgccgc tgattcgata
    53821 taaaccgctg cgggcgctgc gcccaccagg ccggagaata ttgaacttac tgagtcactg
    53881 gtcagggctt tgccgccgtt gatgatctgg ttgtctttat ccaacaaatt cgcctgaccg
    53941 gcgacggcac ggatggtgcc agtagcgtcg aacactgcgg tcatcaccaa tgccagtaca
    54001 ctcggaagta cagttggctg gagtgcgccc ataatatcga ggctgaaaat cagagactta
    54061 ccatcttcgc cagtcaggct tggcatcgcc accagaccgt ggtatttcac cgctgggtca
    54121 aagattaagc cgatgatcga aattgcaata atcaccaaca agatcccgcc gggtacgcga
    54181 cacttctcca gaccgaagat gaccgccagc cccagcaagc tcatcatcac cgggaaggag
    54241 gtaaacgcac cgagcgccac tggcaagcct tcaatcgggt ttttgataac cattcccaca
    54301 ccgttagcag caatcagcag cagaaacagc ccgataccga tacctgtacc gtgagcgata
    54361 cccatcggca aattacgtaa gatccaggta cgcacaccgg ttacggaaat ggcggtgaag
    54421 atgacgccca tcagaaatac cgcgcccagt gcgacaggaa cgctaatttg ttgcccgagt
    54481 accagactga atgcggtaaa cgccgtcaag gaaatcgcgc aaccaatcgc cattggcaaa
    54541 ttggcccata atcccatcag caacgagccg aagcccgcga ccagacaggt ggcaacaaac
    54601 acagctgcgg gaggaaaacc tgctttgccc agcattcccg gaacgacgat aacggaataa
    54661 accatagcca gaaaggtcgt taagccagcc agtacttcct gacgaacggt actgccacga
    54721 gcggtaattt taaaataatt atccagcgcg ccctgtggct ttggtgcgtc cggtgtttgt
    54781 aggatgtctc cagacataat gatgtcctca cgaggatgtt aaagttttat tattagttgc
    54841 gttcgtacac cagacgacca tcaacgtagg tgcggtagat cgaacggtca tcgcccaacg
    54901 tcatcatcac gaacaatttg tcgactaaag aaacagagtt gtcatagcgc agctgctgta
    54961 gcggagtggc ggtgggttcc atcaccacga aatcagcctc tttgccaggt aaaaagttgc
    55021 caatcaaatc gtcaaggccc agagatttcg ctccgccgag cgtggccagg taaaacgctt
    55081 catatgccga gaggcgatag ccttgtaatt gcaacacttt gtaggcttcg ttcagcgttt
    55141 gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc catgcccact ttgactttct
    55201 tctgccatgc ttttttcaag ttgaataagc cgctgccgag gtaaaggttg gaggtcggac
    55261 agaaagcaat gctggatttg gtttcgctga gacgatccca ctctttttct tcgagatgga
    55321 cgcagtgagc aaagacacag tttttaccgg tcaggccgta ctgatggtaa acatccagat
    55381 aaccatcatg gtcaggataa agcgatttca cccaggcaat ttcatctttg ttttcacaga
    55441 gatgggtatg tacccacgta tccggatatt cctctttcag gcgttgcgcc atcgccatct
    55501 gttcaggaga agaagtaggg gcgaagcgtg gtgtaatcgc atatagcaga cgaccatttt
    55561 tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct gctttcggca gtgtcgagca
    55621 gataatccgg tgcgttacgg tccatcatca ccttaccggc aatcatacgc atattgatat
    55681 gactggcggc ttcaaacagc gcatcaacgg attgcggatg aacagtgcca aacaccagcg
    55741 ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa cgccgacatt tcgcgggcgt
    55801 actctaaatc ctcataacga cgttcagtag ggaaggtgtg tttattcaac cactccagca
    55861 attgctcacc ataggccccc accatttcac tttgcggata atggatatgt gtatcgacaa
    55921 agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg aatggtgtca ggaatttgat
    55981 gttttccgtt ttcccattcg ccaaaccatt ccacttttcc ctgtttaatg agtaataaac
    56041 catcctcaat aaaccgcagc gcagaggcaa tctcttccgg gttatcgacc gtacgggtga
    56101 catcaataaa actgcctcgt accgctttta acgtgtgttc tcctgacatc attgactcct
    56161 ttactgttaa tccatctctt ccgctgaggt aataccaggc agaacgtttt cctgtcggta
    56221 gccaccaggg aggataatat ttaataaaat cgcagttaac ccgccagcac aaatagggtt
    56281 ttcaactaat acataaatag aggctggcaa tattttaaaa atttcgggat cgtaggagac
    56341 gccaagccct aaacctaaag aagtggcgac aataagtgtt tcacgccgct ttaatccgtt
    56401 ggtgatgatg atgcgaatcc cagcgatggc aatcatggaa aacatcaacg tcattgcgcc
    56461 tcccagaact gccgagggaa tggtcgtgaa gaagccgcca atcatcggaa ataagccgag
    56521 gataaccagc attaccgcga tggttcgccc gacataacgt gaagcgacgc cagtcatctg
    56581 aataacccca ttattttgcg caaacgtggt taatggtaat gaaccaacag cggaggcgat
    56641 aacagaaacc agaccatctg ccagcacgcc gcctttcagt cgggactgat actcttcccc
    56701 ctgaatgggg cggcgggaaa ccattgccgt ggcggtgata tcgccgactg cttccagcac
    56761 gctaagcaga taaatcgtgc caaccaccag gaactgatgg aagctaaaac taaagccgta
    56821 tttgaacgga tgcgggatgg tgattaacgg caaattgcgc atactgctga aatccaccat
    56881 gcccaggcat aacgatgcga tatagccgac acatagccca atggcgatcc ctcccatgcg
    56941 tagcaacgga ctgcgacagc agttaaagcc gatcaccaca attaaaacca ataaaccaac
    57001 gccgagatgt tcgtaattgc cgaacgtacc gctgcttttg gctgcaaatc ctccaccaaa
    57061 atcgataatg ccgactttaa tcaggcttaa gccgatcatc agtaccacaa taccgctgac
    57121 ggtaggcgta ataacccgac gtaaataggg caagataaat gaagatccga caaccagaaa
    57181 tgcgccaacg aaggagacgc cgagaagcga cgacatgatt aactcttcgt gaaaaccgtc
    57241 gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca aatgaaaaat tgactgactg
    57301 aattgagagt aggccagaac cgacgatgcc gtagcggttt acttgtaacc aggtaccaat
    57361 accagaggcg atcatcgcca tagaaacaag ataggcggtt gtttcagcgg aaagctgtaa
    57421 ggccgcaccc acgattaacg cgggggttac catcggaacg aaaattgcca acagatgggt
    57481 aatggcacca acgagagcct gatgaaaggg agggcgatcc tccagttcaa atataaggtc
    57541 agaacctgca tggtttatat cagacatccc ttcactcctt ttaaagtaaa tttgatcatc
    57601 tgaattagta aaataaattc ccacgaattt ggggcgcagg acgccttgag aacggcttat
    57661 ctcattttcg cagtcactat ttagtcatcg ttcaaacaga gaaacaggcc gccgaagcgg
    57721 ccatttatta cttatatttt ttccagcgcg gtgagtattt tctccggtgt gaaatgccat
    57781 tcgcgtaacc agatgccgca ggcatcgtga attgcggtag caatcgccgg agctgcgcca
    57841 tttacaccaa tttccgagat cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc
    57901 accaacacag cgcggaaatc acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt
    57961 aaatcacgcg ttaacgggtg accttcggcg tcataaatga tctcttcgct catactgtgg
    58021 ccgatagcac gcagggtggc accgtagatt tgtcccaacg ctaactctgg attgaccggt
    58081 gtaccgcagt ccagcaaggc gtagaattta tccaggcgga tttcacccgt acgcgtgttg
    58141 acggcaactt cagcgaagtt tgcgccatac gggaaagcga aatcaggcgt gatataactg
    58201 ccagttccca ccagtgaacc aaagccggtg ccggtttcgc ctttatgggc aatatccccg
    58261 aaactaactt cgcctttctt gccgcgcacg acgcccggcg ttgctagttg aacatctgcc
    58321 actggctcac ccaacatttg cgcgccgtgg aacagaattt tctcccgcag attttccgct
    58381 gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg aggcatatgc gcctttatca
    58441 aacaacgcat gatcggtatc accggagata acatgcacgt cctgcggtgg gcagtgcagc
    58501 acttctgctg ccagtttcgt cactacagta tccagaccag taccaatatc cgcaccgcca
    58561 gaatgaacga taaaggtgcc gtccgattcc agtttgatca tgcagttagc ctgatcgata
    58621 tccgggatac ccgatttctg catgataatg gcgacgccgc gaccgatgtg ccagtcacca
    58681 ttttgcggtt tcggtgaaga ccattggatc atctcgcgac cctgacgcag gatctcttcc
    58741 agtgcgcagc tggcggcgga aggaacggag gtcggcgctt taccttcacc gattgcaccg
    58801 agaattttca gctcttgccc ttcgtgtacc cggttacgtt cgataatttc cagttggtcg
    58861 atctgtaact gttctgccag ttccgctaat gccatggtga tagcgaagtt acctttcggt
    58921 gcgccataac cctgataagc accatttggg caaatgttgc tgtagtaggt ggtgacctgg
    58981 aaatcgacgt tatcgcacgg atataagggc agcgacagcg ccggtccgtt acacggtacg
    59041 gtgagtgagt ggttgccgta agggccagtg ttggcgcgga aatccatctt cactgccgtc
    59101 aggcgaccat cttttttcgc tcccagtttg acggtgactt tcgcgacgtg acgagaggtg
    59161 ttagcaataa actcttcttc acgggtgtag cggaacagta ccggacgccc ggtcacgcag
    59221 gttgcccatg cgcacacttc ttccagcagg atgtcctgtt tggaaccaaa accgccgcca
    59281 actcgctctt taatgacatg aactttatgc tgtttcatgc ccacgaggcg cgcgacctgg
    59341 cggcgtaagt gccatggtac ctgggtggag gcgtggataa ccagacgatc gccatccatg
    59401 cgggtaaagc agatatgtgt ttcagtcggg cactgctgcg cctgcgttga gttataggtt
    59461 cgctcaatga tcacatcggc atcggcaaag cctttgtcca tatcgccaat atgaccatga
    59521 atactggcgg cgatattttt gcgtggacga gaaccgatcg ggaagttgat gatcatatgc
    59581 tcgccgcgct gggctgcatg gctgttatcg tcttccagag tatctggcgc accagcaaca
    59641 tacaccaccg gttcatcgtg cacgacaggc gcatcttccg ccattgcttc gtcgatcgac
    59701 attaccggtt taagcacttc atattcaacg tcgatgagct tcaatgcttc aagcgcaatt
    59761 tcttcacttt cagcgacgac cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg
    59821 aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct gaccacccgg ggtatagtag
    59881 atatccgggc aattcaggtg agtaataacg tgaacgacgc ccggtaaggc ttcagctttg
    59941 ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg
    60001 caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg
    60061 gcatcagttt taggataatg cttgccaatg acttcaaggt cgtcacggaa agtcggagcg
    60121 atatcaatgg tggcctgcgg attattttta cgtgcaaccg ccagttcaat gacctgatag
    60181 tattgctgcc agccagcatc gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg
    60241 cgggtaggtg cggcgatacg atcgagaaga tcggtgataa tcagagccgc agctggatcg
    60301 ttataaccag actgcaccac gccaacatca accattgcct gttgaaccag acttaactcg
    60361 ttccatttgc ccagagattc tgcggtacga atatctgcct tctctaactg tgcggcaata
    60421 agcaaggacg cgttaacgat attaccgtta aagattattg cgtcagaacc ggcaaacccg
    60481 aagccatcat cactgttgcg tacagagtgc attcccatgt taaacaacag cttttgcacg
    60541 ttttcgcctg gattaacggt tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc
    60601 atacggcttc ctccccagcc tgttggcagt cggcatacag gtcggctacc agaactcccg
    60661 tgatatagcg tttataggcc acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg
    60721 cgacagcctg ttcaagtgca ttgccttcca gattttgttt ttcgacatcg tgcagacgca
    60781 gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa
    60841 cggctgccgt cacgacggtt aaacctgcct gagaacggct aattttccgg gtcgcacagg
    60901 tgcgatacgg atctttaatg ataatttcgg ttagcaggcg atcgcatggg caggccaggt
    60961 agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag ttcagcatcc agtgccagca
    61021 ggacgggaag cagcaccgac tcttcctggc gggcggcgat ttcaccacca atggtcgact
    61081 gattacgaac atggcgtgag taaacaaaac cgagggcttc acgcagtgct gcaggaataa
    61141 atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc aatccgcagt gcaccgttat
    61201 cccagtcaac ccagtctagc tccagatcct gtaaggaaat ggcaatcttt ttatcggtac
    61261 gggttggtgt agcattgagt ttgctgcccc cggcgaacca gacggcttca tcctggtagc
    61321 ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct gaaaaattgt tcaatcatct
    61381 tctttcctta tttaaccctc ttccctgcgg gaagagggca tctgtcattt atgccagcgc
    61441 atccatccga cgccacatac tggcagcggc ttttctggct tgtgcataaa tggaatcgca
    61501 atcgaagtta aactgacggt cttcatagac catcacacca ttgaccatca cgctgtgaac
    61561 gctgcctgag cccataccga aagcgatatg cccggcgata ttgtctgcca gcagcggcgt
    61621 cggcgagttg taatcgcaaa tggttaaatc agctttgtaa ccggcttcca gaagcccaaa
    61681 tttcgcgcca aagttgcggc tcatcagttc gttaccgtta gtcagggctt tggcaaaact
    61741 gtcaggccac agcggaccac ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc
    61801 aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg
    61861 atggttgtag ccgacatggt tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc
    61921 gcgctgattg agtagggtga tgtcatcttt cgacaagtac agcccatgag cgaccagcgt
    61981 tttgctgtcg atgagatcga attgcgccag tcgtgccagc aggtctttgc cgtaccagtg
    62041 gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga atatgcaaac cacggcctgt
    62101 ggctttcacg gcttcacgca gcatctccag accggcatcc ggcacggtaa acggcgcgtg
    62161 agcaccgata tgtgcttcca ccagatacgg ctcggttgct gctttcttcg cctcatcaat
    62221 ctgacgggca aagcggatgt tttcttctac accttcctgc aactctttga tgccgttgtt
    62281 acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca acttttaaaa atgcgtcgcg
    62341 caatgtggag agcgacccgc cgatatacgc cggagaggca tggtgatcga taaccgatgt
    62401 acatccgctc ttaatcgctt ccagggaaca aatcagtccg ctgtaataga gcgactcttc
    62461 atcaagggcg cgatcgagcc gccaccagag atttttcagc gttgagatga aatccgggca
    62521 gggggcgatg tttgccataa ttccgcggga aagccccgag taaaaatggt tgtgcgagca
    62581 gacaattccc ggcatcacaa tccgactatg catctctttg tagctggcat cggggtagcg
    62641 ttgcgtcagg gcatcgccga tagcgacaat cacgtcattt tcgatagcga tatcaacgcc
    62701 ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat
    62761 tacacctcca cgcggcccag cagataatga tgatgctggt ggacatggct aataatgcgg
    62821 cacatatcgt tcagctccgg tggtacgttg ttgaactgac cttcgctgtc gatgtttaac
    62881 acccagcttt ggttattcag acgcactcgt acccggcaat cttccacaag gaagcctggg
    62941 ttgctgctgt tatcaaagtc ttgcgacagg ctgaagacgg tgattttgtc tttgtatggt
    63001 ttacccttcc acggacagaa ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg
    63061 tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc
    63121 gggcagacat ccacacactt gctgcaaacg tagttacatt caaggcagcg agcagcttcc
    63181 tgcgcgacaa acgcatcacg atcgtcactg ttcaccagcg tgatagagat atcgccttta
    63241 cgttgataga tttccgccgg attgacgttg ttccagtatt tatcgttctg gtgggaacgg
    63301 atattttccc gactaaggat ggcatcggtc gcccgacgcg cggttccgac agcagcgaca
    63361 atggaggatg gtccgcgctg tacgtcgccg atcataaaga cgtcagtcag acgagtttcg
    63421 ccattatggt cgacgtctgg ccagccgttt ttgtccagcg gcacgcccat cgcattcagg
    63481 gcttcagtat cctgctgttc accaatggcg gtgatcaggc tgtctacatg cagtgttact
    63541 gtttcgttgg tttcaaccgg acgacgacga cctttctcat ccggttcgcc aagcgacata
    63601 acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg gattgttcag gaaacggaac
    63661 tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc atgcgggcat ctcttgcagt
    63721 gaacgacggt aaacgatcgt tgctttttct acgcctggaa cgcgtaacgc cgcacgagcg
    63781 cagtccattg cggtgttacc cgccccgaca acgaccacat gtttgcccag cttgagcgct
    63841 gtacccttgt tgtattcacg caggaagggg agtgatttcc agacattttg gttgtcgccc
    63901 gccagtttca caccgctatt tttatcagtg ccggtggcaa tcagaacata atggaagccc
    63961 tgatttttta actgttcaac ggttaaatcg ggtgggcagc catactcaaa tttcacgccg
    64021 tgatcggcaa caaaatcgat atcgtgctga attaactctg caggaatacg gaactgaggg
    64081 atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt caaacagcgt aaccggatgg
    64141 cccgctctgg caaggaagta acctgctgcc agacccgccg gacctgcacc aatcacggca
    64201 accggatggc gtgaaccaga accagctggt ttgtgccaac gttgcttata ttcatcccaa
    64261 cctttttcca gcgcgacttt tttcagttcg cggatattca gcgcactgtc gtaatccagg
    64321 cgggtacagt tgtattggca ctggtgatcg caaatatgac cggtaatggc aggtagggcg
    64381 ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt gttcgccaag cagacggatg
    64441 tattccggaa tatcttgctt gatagcgcag gcagtaacac agggggcaac gtagcagtcg
    64501 gtcagcggca ggtcttctgc cacttcaata cgctcttctg gcttccagtg tttctgggtg
    64561 tattccatgg ttaacgcgtc tgctgccagt ctgttcagtc gttcgacgtc aacatggtca
    64621 agtccccagg cgtcggagcc ttccagctcg cgcatgcagg cacttaagcg cagatagccg
    64681 ccaggtttca gcaggtcggt tgccatagta atagggcgaa tacctgtatc aaaaatatcg
    64741 cggatagtca gctgactggc accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg
    64801 cgagagagaa ctgctgcaac attgatggag agcgggaaca gcgcacggcc tgacatatac
    64861 atctcttcac cgggcagtgc gcctttattg ttgatggtgc cgagagtgtt agtcagtttt
    64921 acgccaaagc cgagtgattt ttcttttgcc agtgccatca ggcgttccag catttccagc
    64981 gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa
    65041 ccgcagacat cgaggatctc acgaacacgc gcgtacccca gtaaggttgg gttaagtttc
    65101 acaaaggtgt tgagcccttt ttcttccagc atgtagcggc aaatggcttc gatttcatgc
    65161 ggaggacagc cgtgcatggt ggagagggtg acgccctgca ccatactggt ggggatgcga
    65221 gtgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg
    65281 tcatcctgga gtaatttatt cagcgtatcg cgatattgcg cgaatttcgg atggtcagat
    65341 gcgtccatca tattgtcgat gaactgttgc atcggcggtt gcttaatacc ttcgaggttg
    65401 taaccgacac tcatattaaa gatgaacgat ttaccggaat cagaaggctg gaagatcgct
    65461 tcgagaaggt gcagggcaaa ccaggctttg aggtattcat cccaggcttt aagcagggta
    65521 aattcggtag accattcggt gttaaaacac tcgtcttcgg catcgataca gggcttttcc
    65581 agctccaggc ggtcaagaat ttggacggtt tttagttcga tgaagcgtcc gccagtcagc
    65641 caggaagtga caatattttg cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga
    65701 gtggcacagg tttcaccgaa gacgctaaca gttttacctt ttacgggtga gtaaaactgt
    65761 tgctcgggaa taccaaagat tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa
    65821 agttcctcaa acggaatggg acgcataata tcccccataa ccctctcctt agcttataca
    65881 gatatcattg agtgaccggt gttgagaaat ccggtggttt agggggatag agcaacaatc
    65941 gcaccactct tgccaggttt ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta
    66001 aaggcttttt gtgaatgaac tcacaggtgc caaatgagaa ttaaaatcac tcctgaaaat
    66061 tattattgcc agcccattta tcactttttc tcttttctca aaatcagtga ttccttatca
    66121 ttatgataaa gagtgctgga gggattaaat atttgaacgc aatcgtttaa ctacattttc
    66181 taaattttag tatacctttt ttgcgctgta ataaagtcgt ccggagtatc aatatctaaa
    66241 attatttctg cattttctat ttcgacggaa taatgagctc ccataaggag tgcctgacgc
    66301 atattggtga cattgggtcg ctggattgcc tgcatcaagc atagttttga cactaaaatc
    66361 ggatggccgg ggatgccatt atggagtggc aggattgcgc catcatttcg taacgaccag
    66421 atttttctaa aaatatcaat ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt
    66481 tctgtttgca ctgcgggtac tgcggccttg actgatgtta gtaaaccctg cgcataatct
    66541 gggttgtgaa taatagtaat attgctctgg ttcgcataac gttcgtgcag ttcattacca
    66601 cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt gatacttgta
    66661 tcaagaattg ttccctgttg ccagggtaac atcattttcc attgccccat tcttgatgat
    66721 aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt atttataggg
    66781 gctaataacg cctgataatt gcgttacttc ctgatggatt aagttgggga taatttatcc
    66841 cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta gtcattgatt
    66901 taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg aaaaccagct
    66961 tgcttttttg gctggcagaa ctgctccaga ctcgcggcag gcgtgtgtta atcactacga
    67021 cgacacatat gtttatgcca aagtcacact ggcccgtggt tttctgtcgt gatcccgcca
    67081 tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc tggaaagcga
    67141 accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg caacgaccag
    67201 agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta aaagcgcctg
    67261 atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg atgggaggac
    67321 atactttggg cgcgaaagtg agcacagaaa atgtccatcg ctggtcgcag tttgctgata
    67381 ttaccgggtt aacacctgat gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc
    67441 accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc
    67501 gtttttctca atgtgagaat gcgattgcac aaagcgagct ccttcaaccg ctgcaacaac
    67561 atgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat
    67621 ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg aggttggtat
    67681 gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg cgatggcgca
    67741 aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag tgcgcgccga
    67801 tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga ttgaagagtc
    67861 gcttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg ctcgtaacgg
    67921 tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca gcgtccatgg
    67981 tatgcgtcca cgtctggtat tgattggcgc ggggcatgtc aaccgggcga tagcccagag
    68041 tgcggcgcta ttaggatttg atattgccgt tgctgatatt tatcgcgaaa gcctcaatcc
    68101 tgaactattc ccaccatcaa ccacgcttct ccatgctgag tcgtttggtg cggcagtgga
    68161 agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc aggatcgtga
    68221 agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg caagtcgtcg
    68281 caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtggctgaag aacatattgc
    68341 ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg agatcgccat
    68401 cagcgtgctg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa
    68461 accttctcat ccttccggac accagctggt ggtgattcgc ggtgcgggag atatcgccag
    68521 tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa
    68581 accgacagtg attcgttgta ccgtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac
    68641 ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg gaagcgatga aacttaccga
    68701 acgcggattc atccctgtga tggtagatcc cgcctgttca ttgcttgatg aactgaaacc
    68761 gctttgcgtg gtggacgcta ttctggcgaa acagaatttg ggaacgcggg cagatatggc
    68821 accagtaaca atcgcgcttg ggcctggctt tactgctggg aaggattgtc atgcggtaat
    68881 tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg cgcaggagaa
    68941 taccggtgtt cccggcaata ttatggggca taccacccga cgggtgatcc gtgctcctgc
    69001 tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat
    69061 tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc gtggcttgtt
    69121 gaacgacgga ctggcagtgg tcggtggttt taaaattggt gatatcgatc ctcgtggtga
    69181 aacggctgat ttcaccagcg tttctgataa agcccgggcg attggcggcg gcgtacttga
    69241 agcgttaatg atgttgatgc atcagggcgt taaagcgacg aaagaagtgc tggaagtggc
    69301 ttaaatgaaa atggccggat ggtattaccc acccggcctg agactgatga caaacgccaa
    69361 attgcctgat gcgctacgct tatcaggcct acaagtgcat tgcaatctat tgaatttgcg
    69421 atgttttgta ggacggataa ggcgctcgcg ccgcatccag catcaacaaa gcgcacttac
    69481 ttgacaatct ttggccggat ggtattaccc acccggcctt aaaattactg acaaatcacc
    69541 gttccggttt tgccttcaat cccttctttc gctttgctca atacggtaat cagtgcttca
    69601 cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag catagaacct
    69661 ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag gcgatcgagc
    69721 cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt cagaatgatc
    69781 agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt atcgataacc
    69841 gcgctggcac cacgcagatg gttaccttca cgaataacag ggataccacc accgccaacg
    69901 gtgatcacca cctgacctgc atctaccaga gctttaaccg tttctttttc aatgatatca
    69961 accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc tttcagagtg
    70021 taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc gatcggcttg
    70081 gtcgggttga ggaatgccgg gtcgttagca tcgacttcga cctgggtaac cagcgttgct
    70141 acaggtttat taatgccacg agaaagcagt tcttctctta atgcgttttg tagatcgtaa
    70201 ccaatataac cctggcttaa tgcaacacag acagacatcg gcagcattgg cgagtgtgct
    70261 tcagtttttg cggcagcttc aaaggcctgg ttaatcatgc ccacctgcgg cccgttacca
    70321 tgagtgacga taacttcatg tccctgggca attaaatcaa caattgcctg agaagtaatt
    70381 tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg cattcccgcc cagggcgaga
    70441 acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag atccctggca
    70501 gtggttagag cacgggaggg acaaacggtt tgcgtcgcag gaaacgacct cgcccggctt
    70561 tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg
    70621 cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg agatggcgat
    70681 gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg
    70741 ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcatcgcg ctggttaatt
    70801 caacaaaacg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac agtaactgca
    70861 tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct ttagaaattt
    70921 gcaggcgttg agccattgag aacgtgcagt gatcggtcgc caccacgtca atcgcaccat
    70981 cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt gggctaagaa
    71041 tgaacttcat gccatcttct gtatcgtagc tgcgttcgtc caacaggaga tattgtgggc
    71101 aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa tccagaccta
    71161 agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt gccaggttaa
    71221 tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggcg tgatagcgcg
    71281 gcgcggttaa ccctgcggcg ataaactccg cccgcttgct ggcgatagcc gcatcatttt
    71341 ccgggtgcac ggtggtcagc gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct
    71401 cgtcatcgtt gagtttgtat tgataggtta aatagagttt aaaactgctc agcccttcct
    71461 cgaccatcat agggatttcg tcgaggatgg cgtgattaat gtgctggatc acaccgtgaa
    71521 agctgtagtc gatgaccgcc ttatgggcgg catagccacg ataaacctcc agttgatggc
    71581 gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt gtaccgccac
    71641 acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg ccgacatcaa
    71701 tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca gtggcatcaa
    71761 tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca atcccgcttt
    71821 caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg tttttgatca
    71881 atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata agtgaagagt
    71941 aattattcgg ttgccagcca gcttaacggg attgcagcgt acatcgcggc acaggtcacc
    72001 aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt acccgggcca
    72061 aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt tgagaaggtc
    72121 cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac cagtgcttta
    72181 acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt tgggtaaacc
    72241 aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt agctttctgt
    72301 actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc ccaggtcaga
    72361 cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg ggatgtgaag
    72421 aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc cagacgttgg
    72481 gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt gtcaccgcgt
    72541 tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc catacgaccg
    72601 cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa ttccgggcga
    72661 atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc ttcctgcaca
    72721 gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat gattttaccg
    72781 gcataaacca tagatgccat gccgccttcc tggtcggaag tcccgcgacc accgatcagc
    72841 tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt gccaatgccg
    72901 acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat ataaccgaga
    72961 acgttaccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat ctcttcttta
    73021 atacgatgta ctacgcgttt ctcgtcgcag ctttcactgg gaatagcaac catgtcgcgc
    73081 aggaagcgag tcatatccgc ctggtaatct tttgcttttt caagaatcag tttgaatgga
    73141 atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag ggagcagttc
    73201 caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc gcggtagtgc
    73261 ttcacgtcgg tgtcgccttc agtgctgata accagcacta cggcatcttt gttcagcgcc
    73321 agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgccgcgag aacgcccaaa
    73381 ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt tgccgtacgg attacccagc
    73441 acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt ggcgcagtta
    73501 cgtaggattt cccagcccag cgggttaggt tcgccacagg ccagtcccgc catgatggtg
    73561 gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata aatacagtca
    73621 gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctatagac gtcgaccaga
    73681 taacccagca caccaccggc cattgcaccg acaccggctt gcagcagaac gtgcgtcggg
    73741 gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc gtagccttgc
    73801 atgatccagg ttgggatttt ggtgtaacct tcccatgctg tgtcctgtac cacttcccag
    73861 ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt catatccgtg
    73921 acgatgcact cggcaccgag gttcagaatg gcgtcaacgc gttcctgagc agaacctttc
    73981 ggcatgtaaa tcaccgcatt ctgtccgagt tgctgtgctg cccatgccac accgcgcccg
    74041 tggttgccgt cggtggtggt cgcgaaggtc attttttcgc cgatggcatt tttcaggtgc
    74101 tcaaatgaca gcgtttcgat atcaagatga tatttttcac acaataattg agcgatggcg
    74161 tacgcaccgc cgagcatttt gaaggcgttc agaccgaatc gttttgattc gtctttgaca
    74221 agaattttct tcacaccaaa aaggtttgcg agatcgtcca gcgcacaaag cggtgttggg
    74281 cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct ttgcgaaaag
    74341 agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt caatgagaaa
    74401 acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct gatcggcccg
    74461 aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag tgcacctggt
    74521 ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca gctggcttct
    74581 ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcacc ttctttacag
    74641 gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc atcacgggtc
    74701 agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg tgccagacac
    74761 tgtttttcca gtgcttttaa gccttcgtgg tcgttcgcac gcagcaattc agtacgctct
    74821 tccatcactt tgtaaggtgc ccatgacttc ggataaacaa tgtctgcgtc tttgaaggct
    74881 tcttccatgc tggtgacctg acggaagcta ccaccggagg ctttagcatt gtttttcgcc
    74941 acttcaacca catccgggat caggtcgtag ccttccggat gggccagggt gacattcata
    75001 ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt gccatagctt
    75061 ggagagtagg cccaggtcat ggcgattttt ttacctttca ggttttccag tgaaccaaag
    75121 tgttcacgta accatgccag gtcagccatt gactgagtcg ggtggtcaat atcgcattgc
    75181 aggttcacta aagccggacg ttgcggcagc acgccctgct tgtaaccgtc atcaagtgca
    75241 gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc gcgaatacca
    75301 atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc gccgtgagcg
    75361 atttgtgatt tgccttcgtc gagatcttgc tgtgcaaggc cgagcaggtt aagcgcggaa
    75421 gcataagaga agcgggtacg ggtggagttg tcgcggaata cggaaatacc taatccacta
    75481 ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc
    75541 agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga
    75601 aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag
    75661 tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg
    75721 gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg
    75781 caaaagacta atttgacgga taatgtgtgg ggttgatcac aaattgatgc cagcttcggg
    75841 ttgtttgtaa aatacgaaaa cgtttggctt tttttattgc gttatttatt tgctgctaag
    75901 tatcagaaat aagataaagg ggcaggctat ccctcgtcag tttgataaac tcaccgccgt
    75961 aattctcata ttgatataga aatgtgatat ctcttccata atttcattag aaagtgataa
    76021 aaatcttatg tttttatcaa agccaatttc gcctgagtta atatcatcat gatctgtgaa
    76081 ccatcaacgt cttcaatgat cgacttttaa gtaagtctgg aaggtaaagc tatggagctt
    76141 gctactacgc agtcagtatt gatgcaaatt caaccgacaa ttcagcgttt tgccagaatg
    76201 cttgccagcg ttttgcagct tgaggttgag atcgttgatg aaaacttgtg tcgcgtcgcc
    76261 ggaacgggcg cgtatgggaa gtttcttggt cgccagttga gcggcaactc acgcctgctc
    76321 cgccacgtcc tggaaacgaa aactgaaaaa gttgtgacac agtctcgctt cgatcccctt
    76381 tgcgaaggtt gcgatagtaa agaaaattgc cgcgaaaaag catttctggg tacgcctgtc
    76441 attttacagg atcgttgtgt tggggtgata agtttgattg ccgttaccca cgagcaacaa
    76501 gagcatatca gtgataattt acgcgaattt tctgattatg ttcgtcatat atccaccatt
    76561 tttgtttcga aacttctgga ggatcagggg ccaggagata acatcagtaa aatattcgcg
    76621 accatgatcg ataatatgga tcagggcgta ttagttgtag atgacgaaaa tcgggttcag
    76681 tttgttaatc agactgcctt aaaaacactt ggtgttgtgc aaaataatat tattgggaaa
    76741 cctatccgtt tcagaccatt aacatttgag agtaatttta cccatggaca tatgcagcat
    76801 attgtttcgt gggacgataa aagtgaatta atcattggtc aattgcataa cattcagggc
    76861 cgacaattat ttttaatggc gtttcaccaa tcgcatacca gtttttctgt agcaaatgca
    76921 cctgatgaac cgcatattga acaattggtt ggcgagtgcc gtgttatgcg gcaattaaaa
    76981 cgactcatta gccgtattgc acccagccca tccagcgtta tggtggttgg tgaaagcggc
    77041 acgggtaaag aagttgtcgc ccgtgcaatc cataagttga gcggaagacg gaataaaccc
    77101 tttattgcta tcaactgtgc cgcgattccg gagcagcttc tggagagcga actgttcggt
    77161 tatgttaaag gcgcatttac tggcgcttct gccaacggta aaacagggtt gattcaggcg
    77221 gcgaatacgg gcacgctgtt tctcgatgaa ataggtgata tgccattaat gttgcaggct
    77281 aaattactgc gcgctattga ggcacgtgaa attctgccga ttggtgccag tagcccaata
    77341 caagtcgaca ttcgcatcat ttctgcaact aaccagaatt tggcccagtt cattgccgaa
    77401 ggtaaattcc gcgaagatct cttctaccga cttaatgtta tcccgataac tctgccaccg
    77461 ctgcgtgaac gtcaggaaga tattgaacta ctggtgcatt actttttaca tctgcatacc
    77521 cgtcgtctgg gatcggttta tcctggcatt gctcccgatg tcgtcgaaat attgcgtaag
    77581 catcgttggc ccggaaacct gcgcgagtta agcaatttga tggaatatct ggttaacgtt
    77641 gttccttcag gtgaagttat cgacagcacg ctattgccgc caaatctgct gaataatggc
    77701 acaacggagc aaagtgatgt aacagaggtc actgaggcac acctgtcact cgatgatgcg
    77761 ggcggcacgg cgctggagga gatggaaaag caaatgatcc gcgaggcgct ttcacgtcat
    77821 aacagcaaga agcaagttgc tgatgaactg ggcatcggca ttgctacgct ctatcgcaag
    77881 attaagaaat atgagttgtt aaacacataa cctcagtgaa tccggcctga attcaggccg
    77941 gatatccttt tacttcgttt tctcgcaatc cagaactgta tttacaatca tctgataccc
    78001 agtgcagcga caaagatttc ccgccagtcc gcgacgaatt tccgtaatgg ttaatggctt
    78061 ctcgcgtggt ttcgccagca ttgccgtggt agccataatc aggccaggcg tacaaaaccc
    78121 gcactgcact gcgccggatt tcgcataagc ctgctgaaca tgagaaagtt ttccgccttt
    78181 cgcttcacct tcaagcgtgc ggatctcttt tccttcagcc caggcggcaa ggtataagca
    78241 actgtctatt gctgtgccgt cgaccagcac cgtacaggca ccacattcac ccacgcagca
    78301 cccttgtttg acacttagca gtccttgttc gcggagtaat tccgagagcg gcgtgcctgg
    78361 tgcggcatga agctgaaaag gcatcccgtt aatggtgcat tcgatggtaa ttgtttcgct
    78421 gtgattcatt gcaattttcc ccccgccgcg gcgacggctt cgctaatcac ttttttggtc
    78481 atcgtctgga tgagatgcag acgaaactct ttactggccc gccatgaaga acgcggggcg
    78541 acatcttgca ggacagattc gctgatagct tccagcgttt gcaggtttaa tggcgcattt
    78601 tgtgcagtct gttcggcatg ttggcagcga atcggcgttg gcgcggcaac accaaatgcc
    78661 aggcgtaatt cgctgaaatt gccgttatcc agtcggcaat gtgcggcaca gccaatcgtt
    78721 gaaatatcca ttgcgtcgcg catggcatat ttaaaatgcg cgctgcccac gtgttctttc
    78781 ggctggggcg gaaaatgaaa ggcgacaagg atttcgtcat gctcaagaga cactttgccc
    78841 ggcccggtgt gaaagccatt aatcgggacg aaacgaacac tgcgtgggga gtggatctcc
    78901 agtttcgcgt cataaatgag cgttggcgtg gcagaatctg cgctggtggc accgttgcaa
    78961 atatttccac cgtaggtagc gacgttacgg atctgcggtc cagcaatgga cgaggccgca
    79021 gcacataacg ccgggagatg acgttgagtt ataggatctt ctattagctg ggtaaatgtc
    79081 gttgcagagc cgatacgtag cgagccatct tccgccagcg taattccccg cagctccgcc
    79141 agattatgaa tatcaacaat atggcgataa cggtcattgt gatggtggag ctgaatcagt
    79201 acgtcagtgc caccggcgag cagtttggcc tgcgggttgt cagccagcag gttgatggca
    79261 tcggcaaggg ttgctgcgcg atggtaagaa gcaaaatcaa acatgatgtt atcctcaaat
    79321 caatcctgcc agatggaact cttcatataa ccgttttggc gtcagcggca gtgtattgat
    79381 tgcaacaccg gtagccatct tcaccgcgtt acgaatagct gcggcaacag gaattattgg
    79441 tggctcaccc agtgacttat gtccgtatgc ggattgcggc tcattgattt cgacgaacgc
    79501 gctttccagt tgtggcagat ccggcatggt cggcattttg taatccagca gattggggtt
    79561 acggaccacg ccgcttttcg catcgatgat catctcttca aatagcgccc agccaatgcc
    79621 cattcccatt ccgccgtgta cctgaccttc tgccagtagc ggattaagaa tatgccccga
    79681 atcatgaacg ttgaggatgc ggttgatggt gactttgcac agtgcaatat cgaccgtcag
    79741 atcaacaaag gtacagccaa acgccggtgg gttagtggtg gttttgatgg agctttcagc
    79801 agagagctgc ccaccgcgtt cagggtggta gaaagcgtcc atcgccaaat cttttaacga
    79861 cattaacggc tcttccggtc gttcaaccag cacgatatgg ccttttatca gggtcagatt
    79921 catcgctgac tgatgtagca tgactgcggc gtgagcgatg attttctctt ttaataacag
    79981 tgccgcactg cgcagcgcag gcgcggcaac atagctctgg cgtgaggcaa atgcgccggg
    80041 atcgaacggc gtaacgtcgg tatcttgtgt tgaaataacg cgaacgtcgc taaccggaac
    80101 ccccacggtt tctgccacca tttgcgagaa gacggtgtcg gcaccctgac cgatttccgt
    80161 cgcgccgctt tgcacgttga tggttccatc ctgattcatc agaaggcgcg cgcctgctat
    80221 ttctacgccg acaggccagg tattagaggt gtagctaaaa caggcgacgc caacgccgcg
    80281 gcgcaaattg ccttgctggt tctggcattc tgcacggcgt ttttcccatt caaagatttt
    80341 ccggcctttt tcaagacact ccggcaaccc tgcgctgtaa atacgtttgc ccgtgagcgg
    80401 attagcatct ccttcgcggg cggcgttgcg taaacgaatt tcaacaggat caatacctaa
    80461 cgctgtcgcg gcgtcatcaa gcatagactc aacggcaaat acgacttgtg gcgcgccata
    80521 accacgcatc gcaccagccg aggggaggtt ggtatagcag gtctttgaac tgtaagcgta
    80581 ggcacaacga ggataaaggt aagcgacttt attccccccg cagaagcgat ggagtgcccg
    80641 tgagatgcat aagcgccggt gttagacaga acatccagac tataaccttt caatgttccg
    80701 tcgcggttca cgcccatttg cccgtcaata gtaaaagcgt ggcgggtacg ggttgcgagg
    80761 aaacactctt cacggctaag ggaaactttc accggaatgc cgccaagctt gctggtcagg
    80821 aatgccgcca ttggctcttc cagtacatcc tgtttattac caaaaccgcc accgacaaat
    80881 ggtttgatga ctcgtacgca tgaccaggga atatccagcg cctgaccaac cacgcggcga
    80941 acaatgtgcg ggatctgggt gctggaaacg atggtaattc gcgagtcatc ctccatccac
    81001 gccagcgatg ttacgctttc catgtgacaa tgttgaataa cgggggtctg atagtgcccc
    81061 tgtacctggt agtcggcggc atcgattgtt tgttggacat tgcccgtcga catcgtgctt
    81121 tgtttcagta aattgccgcc gttatggatt ggtgcagcgt cctctgccag cgccgcttct
    81181 ggcgtggtga taacgggtaa ttcttgccac tcaatgctga ccaattgcgc cgctttttct
    81241 gccgtgagtt catcgcgggc cacgacgatg gcaacggcgt caccatgatg acgaacatgg
    81301 cgagttagca gtgcgcgatc ggcggtatcg cgcttgtttt cgtcaagtgt ccatgcatgc
    81361 ccagctgtag cgaatgggat atcaggcaca tcttcccagg taaaaatcgc cagtacgcct
    81421 ggtaaacttc tggcttgttc atcattaata cttacggcat aaccatgtgc gatagggcta
    81481 cgtacatatt tcgcataaca catgcccgcc ataacataat cgtcagtata tcgtgcccgc
    81541 ccggtgacct tagcaatggc atcaacgcgc atgcatgatt cacccgtagc ggttgcttcc
    81601 cgcgcttcca tagaaatccc ctcgatttga acatttggtt cttatggcaa agatttagtc
    81661 taagcaggca agatttacgc cagaaacgac gataagcgtt aggggaggaa ggaagtgtga
    81721 tgacgttaaa aaaacacaga tatgatacga aacaggactc tgaatagggg cagtgatacg
    81781 tatcaatatg agtggcgata aaatcaatgt gataaattgg gattattacg catcaggatg
    81841 ggaaataggg taaaaaagtc aggaaattcg atacctctac agcgaaaatt ttgctgatga
    81901 tacttccctt tgccaaatga aattaacctc tttctctatt accgcgtttt tccagaagca
    81961 agagattggg tatcaagagg ctggctgcta tgatatgacg tcttgttttt taagcgagga
    82021 aagattttga gtgcgggacg cctgaataaa aaatctctgg gtatcgtgat gttgttatcg
    82081 gttggactgc ttttggcggg ctgttcgggt agcaaatcat ccgatacagg aacgtattcc
    82141 ggctccgttt acaccgtgaa acggggggat acgctatatc gtatttcgcg caccacggga
    82201 accagcgtaa aagaactggc gcgactgaac ggcatttccc ccccttacac tattgaagta
    82261 ggtcagaaac taaaactggg tggggcgaaa agtagcagta gtacacgtaa atcaaccgcc
    82321 aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag cggtaccgaa atcttcctgg
    82381 ccgccagtag ggcaacgttg ttggttatgg ccaacgacag ggaaagttat catgccgtat
    82441 tcgacagcag atggcggcaa taaagggatt gatatctcag ctccacgggg tacacctatt
    82501 tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc agctgcgtgg ctacggtaat
    82561 ctcatcatga ttaaacacag tgaagattac attacggctt acgcccataa tgacacgatg
    82621 ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa tcgccaccat ggggagcacg
    82681 gatgcggcat ctgttcgcct gcatttccag attcgttacc gtgcaacggc aattgatccg
    82741 ctacgttact tgccgccgca gggcagcaag ccaaaatgct gatggcgaat taatcagcag
    82801 tcagcagcgc ggcacttgct aaggagagcg taaggtttat aatgccttac gcatctcgaa
    82861 gcgggcgtag ttcaatggta gaacgagagc ttcccaagct ctatacgagg gttcgattcc
    82921 cttcgcccgc tccaatttat cttcacctca tcaacctgcc atttttgttg tatctgattt
    82981 tctatattat cttaattcaa tcgcttcaga cttttcgcaa tcgccgtaac acttccccat
    83041 ttttttgtaa ataaaaagcg tttacgcaat gcaatccttt tgtaacaacc caggtaaaat
    83101 accttatttt caagttgtta aataagtggt tatctacgct tttaacaaac gattgatgga
    83161 atattttatg aaagggaaat ctgcattaac tcttctgctc gctggtatat tttcgtgcgg
    83221 cacatgcctg gctacagggg cagaagtgac gagtgaatct gtatttaaca ttcttaattc
    83281 cacgggggca gcaacagata aaagttattt atcgttaaat ccagataagt atcctaatta
    83341 tcgtcttctg attcactcag ccaaattaca aaatgaaata aagagccact ataccaaaga
    83401 cgaaattcaa ggattgctaa cattaacaga aaacacacga aagttaacgt tgacggagaa
    83461 accctgggga acgtttattc ttgcttctac tttcgaagat gacaaaacag cggcagaaac
    83521 ccactatgat gctgtctggc tgagggatag tttatggggc tatatggcat tagtatcaga
    83581 ccagggaaat agtgtagccg caaaaaaggt tctgctcacc ctgtgggatt atatgtctac
    83641 gcccgaccag attaagcgga tgcaggacat cattagcaac ccaaaacgac tggatggtat
    83701 tcccgggcaa atgaatgctg tgcatatccg atttgacagc aactcccccg tgatggcaga
    83761 tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa caaaatgatg cgttaggtct
    83821 ttatcttgat ctccttatac aagcaatcaa tactagtact attaacgctg aagattggca
    83881 aaaaggtgac cgattaaaat cggttgcgct gctgattgct tatctggata aagccaattt
    83941 ctatgttatg gaagattccg gagcgtggga ggaagatgcc cgattaaaca catcttccgt
    84001 tgcactggtg acctctggac tggaacgttt gtcaaaccta ctctcgaaga aagactctgt
    84061 ttttgtctct gatttactac gcgaagctaa agtcaatgaa ctggatgaaa ctctttcaac
    84121 aacgaggttg aaccacctca tagataaagg ttatgaaaga ataacgttac agcttgattt
    84181 agggggagaa tccccaggtt atctggaaaa agataaacat tacagagaag ccgatgcggc
    84241 tttacttaat gttatttatc ctgcaaatct ttcaaagata aacaccagaa gaaaagagca
    84301 agttctaaaa atagttaaaa aactagcagg tccgtatggt ataaaaagat acgagaagga
    84361 taattatcag tcggcaaatt tttggttcaa tgatattaaa accgataccg atcaaaattc
    84421 gcatgcaaaa agagaaaaga gcttcattcc atcaacagaa gcagaatggt tttttgactc
    84481 atggtatgca aaatcagcag caattgttta taaagaatca agaaaagaag agtatttgaa
    84541 tgactcagta caatttatga atagatcatt ggcccaaata acaggcgaga atatgattgg
    84601 tgcaaatggc aggagcgtac cagaaatggc acttcccgaa agctataact atatccacaa
    84661 gtcgggaact ttgcatgaag cgccaagccc tatcattccg ctaaattggt cgaaagcttc
    84721 aatgactctc atgttgaaag agatgtccaa ccttatcaat gatgagggaa ttaaataatt
    84781 ttcataatga ggcggtcctg gcttaaaagg accgctcaaa gagaaatcga aaggggaggt
    84841 aaaagtgatc aataaattac tgttagctta cctgataggt ctggttgtga catcgacttt
    84901 aatatttatc ttctcggaag aaaaagtaac atatcggttg tttgctgcaa taattacagg
    84961 tttaacctgg ccgcttagtc tcattccttc cattataagt ctcatgattc gtaaatccga
    85021 ctaacaagaa aattatatga tcgattaaga aa
//
LOCUS       DAPGRN010000005        83935 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPGRN010000005 DAPGRN010000000
VERSION     DAPGRN010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.005"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..556
                     /locus_tag="RHW33_000378"
     CDS             <1..556
                     /locus_tag="RHW33_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDV1643927.1"
                     /translation="CGYNDIQTYDEQVNASWSEVLNQYQRRADLIPNLVASIKGYSSH
                     ERDVLEAVTLARSQANRASSDLQQTPGDEQKLQAWQQAQAQVTRTLGQLTIISERYPE
                     LKSQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKFPAVLTAKVMDYTPKKN
                     YLPDDVAAVSKAPTIDFSQNANAH"
     gene            570..1448
                     /locus_tag="RHW33_000379"
     CDS             570..1448
                     /locus_tag="RHW33_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV1643928.1"
                     /translation="MRNFIFLMAFFCSSVFATQIPVPESPKYVNDLTGTLTNSEVNTL
                     TKQIKALTQKSHAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGIITDAQSGQIIRNSIIPAFKNGDLAGGLQKGLSDIENRLAGNNLA
                     TIAPADHPLPFSGWWALLAWAIVLTFISARGYIKTLGVICFAAIVLAFVLPIAGFSGS
                     WGVLTTLLCFATPFLAVAIAFTPFGKKVRDLMRDANQPSRHTRSNSFSHYSSSSSSSF
                     DNDNFSGGGGGASGRW"
     gene            1463..2611
                     /locus_tag="RHW33_000380"
     CDS             1463..2611
                     /locus_tag="RHW33_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV1643929.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVNDLTGTLTTEEQSVLNQQLKT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANISPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFASRPRRGIWMISMITPMVWFFSFQ
                     DDVIIRRVSVVCFSLLLATTCKQRLAVVFNLCRVFPMMLAPKNKNAKVKKQKKTPQVR
                     KSGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHSRSSSRNSFRGGGGSSGGGGSSGRW"
     gene            2608..3522
                     /locus_tag="RHW33_000381"
     CDS             2608..3522
                     /locus_tag="RHW33_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311665.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV1643930.1"
                     /translation="MIVMRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQ
                     QSLTQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDSWKLGDKQRNDGILLLVA
                     WEDHAVRIEVGYGLEGVVTDLQAAKIIRDILIPAFKSDDLMGGLTLASENIGALLLNG
                     ELPEDRGDYYSINPPIPLSLAVIILLAVLSYFIVFTDPSNLPWITLTGAIYGMVFLYV
                     AEPGPWTNLIVACGMLTPFAIVPLVIFWLIVNKKLRAKYKKLSKERASRKGSSSSSSG
                     GGSSGGGSGGGFSGGGGSSGGGGASGRW"
     gene            complement(3582..4880)
                     /gene="eno"
                     /locus_tag="RHW33_000382"
     CDS             complement(3582..4880)
                     /gene="eno"
                     /locus_tag="RHW33_000382"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV1643931.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4968..6605)
                     /gene="pyrG"
                     /locus_tag="RHW33_000383"
     CDS             complement(4968..6605)
                     /gene="pyrG"
                     /locus_tag="RHW33_000383"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV1643932.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6833..7624)
                     /gene="mazG"
                     /locus_tag="RHW33_000384"
     CDS             complement(6833..7624)
                     /gene="mazG"
                     /locus_tag="RHW33_000384"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV1643933.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFSSIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     GSAGNSREVLARWEEIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFELRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7696..8031)
                     /gene="mazF"
                     /locus_tag="RHW33_000385"
     CDS             complement(7696..8031)
                     /gene="mazF"
                     /locus_tag="RHW33_000385"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV1643934.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRAKGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8031..8279)
                     /gene="mazE"
                     /locus_tag="RHW33_000386"
     CDS             complement(8031..8279)
                     /gene="mazE"
                     /locus_tag="RHW33_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV1643935.1"
                     /translation="MIHSSIKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(8357..10591)
                     /locus_tag="RHW33_000387"
     CDS             complement(8357..10591)
                     /locus_tag="RHW33_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV1643936.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10639..11940)
                     /gene="rlmD"
                     /locus_tag="RHW33_000388"
     CDS             complement(10639..11940)
                     /gene="rlmD"
                     /locus_tag="RHW33_000388"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV1643937.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYSRAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11997..14753
                     /gene="barA"
                     /locus_tag="RHW33_000389"
     CDS             11997..14753
                     /gene="barA"
                     /locus_tag="RHW33_000389"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV1643938.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14985..16325)
                     /gene="gudD"
                     /locus_tag="RHW33_000390"
     CDS             complement(14985..16325)
                     /gene="gudD"
                     /locus_tag="RHW33_000390"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV1643939.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(16346..17686)
                     /locus_tag="RHW33_000391"
     CDS             complement(16346..17686)
                     /locus_tag="RHW33_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV1643940.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17688..19040)
                     /gene="gudP"
                     /locus_tag="RHW33_000392"
     CDS             complement(17688..19040)
                     /gene="gudP"
                     /locus_tag="RHW33_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV1643941.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(19475..19924)
                     /locus_tag="RHW33_000393"
     CDS             complement(19475..19924)
                     /locus_tag="RHW33_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV1643942.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTTQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFNALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19942..20724)
                     /gene="truC"
                     /locus_tag="RHW33_000394"
     CDS             complement(19942..20724)
                     /gene="truC"
                     /locus_tag="RHW33_000394"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV1643943.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20724..21053)
                     /locus_tag="RHW33_000395"
     CDS             complement(20724..21053)
                     /locus_tag="RHW33_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV1643944.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDVPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21675..22220)
                     /gene="syd"
                     /locus_tag="RHW33_000396"
     CDS             complement(21675..22220)
                     /gene="syd"
                     /locus_tag="RHW33_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV1643945.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            22288..23136
                     /gene="queF"
                     /locus_tag="RHW33_000397"
     CDS             22288..23136
                     /gene="queF"
                     /locus_tag="RHW33_000397"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV1643946.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGEVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            23248..24612
                     /gene="ppnN"
                     /locus_tag="RHW33_000398"
                     /gene_synonym="ygdH"
     CDS             23248..24612
                     /gene="ppnN"
                     /locus_tag="RHW33_000398"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV1643947.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            25169..26458
                     /gene="sdaC"
                     /locus_tag="RHW33_000399"
     CDS             25169..26458
                     /gene="sdaC"
                     /locus_tag="RHW33_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV1643948.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            26516..27883
                     /gene="sdaB"
                     /locus_tag="RHW33_000400"
     CDS             26516..27883
                     /gene="sdaB"
                     /locus_tag="RHW33_000400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV1643949.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            27995..28750
                     /gene="xni"
                     /locus_tag="RHW33_000401"
     CDS             27995..28750
                     /gene="xni"
                     /locus_tag="RHW33_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV1643950.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(28805..29953)
                     /gene="fucO"
                     /locus_tag="RHW33_000402"
     CDS             complement(28805..29953)
                     /gene="fucO"
                     /locus_tag="RHW33_000402"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV1643951.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTHKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(29981..30628)
                     /gene="fucA"
                     /locus_tag="RHW33_000403"
     CDS             complement(29981..30628)
                     /gene="fucA"
                     /locus_tag="RHW33_000403"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV1643952.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            31175..32491
                     /gene="fucP"
                     /locus_tag="RHW33_000404"
     CDS             31175..32491
                     /gene="fucP"
                     /locus_tag="RHW33_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV1643953.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            32524..34299
                     /gene="fucI"
                     /locus_tag="RHW33_000405"
     CDS             32524..34299
                     /gene="fucI"
                     /locus_tag="RHW33_000405"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV1643954.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            34408..35826
                     /gene="fucK"
                     /locus_tag="RHW33_000406"
     CDS             34408..35826
                     /gene="fucK"
                     /locus_tag="RHW33_000406"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV1643955.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            35828..36250
                     /gene="fucU"
                     /locus_tag="RHW33_000407"
     CDS             35828..36250
                     /gene="fucU"
                     /locus_tag="RHW33_000407"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV1643956.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            36308..37039
                     /gene="fucR"
                     /locus_tag="RHW33_000408"
     CDS             36308..37039
                     /gene="fucR"
                     /locus_tag="RHW33_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV1643957.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(37083..38183)
                     /gene="rlmM"
                     /locus_tag="RHW33_000409"
     CDS             complement(37083..38183)
                     /gene="rlmM"
                     /locus_tag="RHW33_000409"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV1643958.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRTALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(38176..38571)
                     /locus_tag="RHW33_000410"
     CDS             complement(38176..38571)
                     /locus_tag="RHW33_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV1643959.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(38590..39507)
                     /gene="gcvA"
                     /locus_tag="RHW33_000411"
     CDS             complement(38590..39507)
                     /gene="gcvA"
                     /locus_tag="RHW33_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV1643960.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKLRFRYEQ"
     gene            complement(39858..40085)
                     /locus_tag="RHW33_000412"
     CDS             complement(39858..40085)
                     /locus_tag="RHW33_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV1643961.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            40277..41482
                     /gene="csdA"
                     /locus_tag="RHW33_000413"
     CDS             40277..41482
                     /gene="csdA"
                     /locus_tag="RHW33_000413"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV1643962.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            41482..41925
                     /gene="csdE"
                     /locus_tag="RHW33_000414"
     CDS             41482..41925
                     /gene="csdE"
                     /locus_tag="RHW33_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV1643963.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(41976..42782)
                     /gene="tcdA"
                     /locus_tag="RHW33_000415"
     CDS             complement(41976..42782)
                     /gene="tcdA"
                     /locus_tag="RHW33_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV1643964.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQHMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(43021..44118)
                     /gene="mltA"
                     /locus_tag="RHW33_000416"
     CDS             complement(43021..44118)
                     /gene="mltA"
                     /locus_tag="RHW33_000416"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV1643965.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            44327..44403
                     /locus_tag="RHW33_000417"
     tRNA            44327..44403
                     /locus_tag="RHW33_000417"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44361..44363,aa:Met,seq:cat)
     gene            complement(44477..45730)
                     /gene="amiC"
                     /locus_tag="RHW33_000418"
     CDS             complement(44477..45730)
                     /gene="amiC"
                     /locus_tag="RHW33_000418"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV1643966.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            45962..47293
                     /gene="argA"
                     /locus_tag="RHW33_000419"
     CDS             45962..47293
                     /gene="argA"
                     /locus_tag="RHW33_000419"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV1643967.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(47355..49181)
                     /gene="recD"
                     /locus_tag="RHW33_000420"
     CDS             complement(47355..49181)
                     /gene="recD"
                     /locus_tag="RHW33_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV1643968.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDANTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(49181..52723)
                     /gene="recB"
                     /locus_tag="RHW33_000421"
     CDS             complement(49181..52723)
                     /gene="recB"
                     /locus_tag="RHW33_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV1643969.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFIFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(52716..55604)
                     /gene="ptrA"
                     /locus_tag="RHW33_000422"
     CDS             complement(52716..55604)
                     /gene="ptrA"
                     /locus_tag="RHW33_000422"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV1643970.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSLGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKSANIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(55780..59148)
                     /gene="recC"
                     /locus_tag="RHW33_000423"
     CDS             complement(55780..59148)
                     /gene="recC"
                     /locus_tag="RHW33_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV1643971.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTNELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMTQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(59161..59484)
                     /locus_tag="RHW33_000424"
     CDS             complement(59161..59484)
                     /locus_tag="RHW33_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV1643972.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(59469..59876)
                     /locus_tag="RHW33_000425"
     CDS             complement(59469..59876)
                     /locus_tag="RHW33_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV1643973.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLIAGYEGVSL
                     WRTGEIIDGNIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(59873..60436)
                     /locus_tag="RHW33_000426"
     CDS             complement(59873..60436)
                     /locus_tag="RHW33_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV1643974.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(60427..60897)
                     /locus_tag="RHW33_000427"
     CDS             complement(60427..60897)
                     /locus_tag="RHW33_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV1643975.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(61082..61876)
                     /gene="thyA"
                     /locus_tag="RHW33_000428"
     CDS             complement(61082..61876)
                     /gene="thyA"
                     /locus_tag="RHW33_000428"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV1643976.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFDIEGYDPHPGIKAPVAI"
     gene            complement(61883..62758)
                     /gene="lgt"
                     /locus_tag="RHW33_000429"
     CDS             complement(61883..62758)
                     /gene="lgt"
                     /locus_tag="RHW33_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV1643977.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(62909..65155)
                     /gene="ptsP"
                     /locus_tag="RHW33_000430"
     CDS             complement(62909..65155)
                     /gene="ptsP"
                     /locus_tag="RHW33_000430"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1643978.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKKTAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(65168..65698)
                     /gene="rppH"
                     /locus_tag="RHW33_000431"
     CDS             complement(65168..65698)
                     /gene="rppH"
                     /locus_tag="RHW33_000431"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV1643979.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            66383..67072
                     /gene="mutH"
                     /locus_tag="RHW33_000432"
     CDS             66383..67072
                     /gene="mutH"
                     /locus_tag="RHW33_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV1643980.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            67141..67854
                     /locus_tag="RHW33_000433"
     CDS             67141..67854
                     /locus_tag="RHW33_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV1643981.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            67992..68210
                     /gene="ygdR"
                     /locus_tag="RHW33_000434"
     CDS             67992..68210
                     /gene="ygdR"
                     /locus_tag="RHW33_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV1643982.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            68318..69358
                     /locus_tag="RHW33_000435"
     CDS             68318..69358
                     /locus_tag="RHW33_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV1643983.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(69391..70584)
                     /gene="lplT"
                     /locus_tag="RHW33_000436"
     CDS             complement(69391..70584)
                     /gene="lplT"
                     /locus_tag="RHW33_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV1643984.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(70577..72736)
                     /gene="aas"
                     /locus_tag="RHW33_000437"
     CDS             complement(70577..72736)
                     /gene="aas"
                     /locus_tag="RHW33_000437"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV1643985.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            73322..74353
                     /gene="galR"
                     /locus_tag="RHW33_000438"
     CDS             73322..74353
                     /gene="galR"
                     /locus_tag="RHW33_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV1643986.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNHPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(74360..75622)
                     /gene="lysA"
                     /locus_tag="RHW33_000439"
     CDS             complement(74360..75622)
                     /gene="lysA"
                     /locus_tag="RHW33_000439"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV1643987.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSALQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAARQQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            75744..76679
                     /gene="lysR"
                     /locus_tag="RHW33_000440"
     CDS             75744..76679
                     /gene="lysR"
                     /locus_tag="RHW33_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV1643988.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFHQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAIKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(76666..77358)
                     /gene="ygeA"
                     /locus_tag="RHW33_000441"
     CDS             complement(76666..77358)
                     /gene="ygeA"
                     /locus_tag="RHW33_000441"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV1643989.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FQEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRTYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(77487..78905)
                     /gene="araE"
                     /locus_tag="RHW33_000442"
     CDS             complement(77487..78905)
                     /gene="araE"
                     /locus_tag="RHW33_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV1643990.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(79220..79981)
                     /gene="kduD"
                     /locus_tag="RHW33_000443"
     CDS             complement(79220..79981)
                     /gene="kduD"
                     /locus_tag="RHW33_000443"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV1643991.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(80011..80847)
                     /gene="kduI"
                     /locus_tag="RHW33_000444"
     CDS             complement(80011..80847)
                     /gene="kduI"
                     /locus_tag="RHW33_000444"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV1643992.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVID
                     LR"
     gene            complement(81134..82315)
                     /locus_tag="RHW33_000445"
     CDS             complement(81134..82315)
                     /locus_tag="RHW33_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV1643993.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            82570..83799
                     /locus_tag="RHW33_000446"
     CDS             82570..83799
                     /locus_tag="RHW33_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1643994.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGIKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        20649 a        22414 c        20437 g        20435 t
ORIGIN      
        1 ctgtggatat aacgatattc aaacctatga tgagcaggtt aacgcctcct ggtctgaggt
       61 attgaatcaa tatcaacgtc gcgccgatct tattcctaat cttgtcgcca gtattaaagg
      121 ttattccagc catgaacgag atgtactgga agccgtaaca ttggctcgta gccaggctaa
      181 tcgcgccagt agcgatcttc agcaaactcc gggagatgaa caaaaattac aagcctggca
      241 acaggctcaa gcgcaggtga ctcgcactct cggtcagtta acgataatta gtgagcgtta
      301 ccctgagtta aaatctcagg aactctatca aaatttaatg gtacagcttg aaggaagtga
      361 aaatcggatt gccgttgcgc gaggaagata tattaaagcc attgaacaat ataatgtcac
      421 tatccgcaaa ttccccgctg tgttaaccgc aaaggtcatg gattacacac caaagaaaaa
      481 ttatttgccg gatgatgtcg ccgcagtaag taaagcccca acaatagatt ttagccagaa
      541 tgctaatgct cattaagcgg ggaagttcta tgcgaaattt tatcttcctt atggctttct
      601 tctgttcatc tgtgtttgcc acacaaattc cagtacctga atcacccaag tatgtgaatg
      661 acttaacggg cacattaaca aacagcgaag taaacaccct gaccaaacag attaaagccc
      721 tgacgcaaaa aagccacgca caactggtcg tgttagtggt tgaaacaaca ggcgatgaaa
      781 ccatcgagca gtacgcaacg cgggtattcg atagctggaa accgggtgat aaagaccgcg
      841 atgatggcgt tctactgtta gtcgcctggc aggatcatac cgtgcgtatt gaaatcggtt
      901 acggactgga aggtattatt acggatgcgc agtccgggca aattattcgc aacagtatta
      961 ttccggcatt taaaaatggt gatcttgctg gcggcttgca aaagggactc agcgatattg
     1021 aaaaccgttt ggcagggaat aatttagcga cgatagcacc ggccgatcac cctttacctt
     1081 ttagcggttg gtgggccttg ttagcctggg cgatagtact gacgtttatt tctgccagag
     1141 gatatatcaa aacgctggga gtaatttgct tcgcagctat tgtactggcc tttgttttac
     1201 ctatagcagg tttcagcgga agctggggag tactcacaac attactctgc ttcgccacac
     1261 cgttcctggc tgtcgccatc gcttttaccc ccttcggcaa aaaagttcgg gatttgatgc
     1321 gtgatgccaa ccagccatca cgccatacaa gaagcaattc tttctcccac tattcatcat
     1381 cgtcatcctc ttcattcgac aatgacaatt ttagcggtgg tggcggtggc gcttccgggc
     1441 gctggtaaca aaaggaattg atatgcgtat ttttctctta cttctgatgc tatgcggttt
     1501 tcaggtattc gctgaacgat tgccttcagt gaatgactta accgggacat taacgacaga
     1561 agaacaaagt gttttaaatc aacaattaaa gactctcgag cagcaaaacc acttccaggt
     1621 ggccgtgttg gttacgccga ccacaggggg caaaaacatt aaacttgcag cctcgcagag
     1681 atacggggta tatcactgga aacccgttgg agagaagcga tatggcgaag gtatcttaat
     1741 tctcgtcgta tggccggaag ggctggcgtc aatgaagatt ggacatggac tggaacagat
     1801 gttgccgccg gaacaagctg cgcaaattgt tcgttaccat atgcagccag aattcgaaaa
     1861 aaacaatctc tttgctggat taacgggcgg aattgaaagc atcgcacagt ttacccatat
     1921 caaagcgaac attagccctt tggatgcttt agccaatcat ctgtttgcta atccgcaacg
     1981 ttcgctgcca tgtcttgcct ggacactgct gatgatcgtt gccatcgttg tcctctggcg
     2041 atttgccagc cgcccacggc gtggcatatg gatgatatcg atgattaccc ctatggtctg
     2101 gtttttctcc tttcaggacg atgtcatcat tcggcgggta tcggtcgttt gttttagttt
     2161 attgttagca acaacctgca agcagcgtct cgcggtagtt tttaatttgt gccgcgtatt
     2221 tcctatgatg ctggccccaa aaaataaaaa cgctaaagtg aaaaaacaaa agaagactcc
     2281 gcaggttcgt aaatcaggac gaaattcatt gtttgtcgtg atgctgataa ttattgtggg
     2341 atggtgcctg gtgttcgcca gtaataaaga tattgcattc atctcaacaa ttattgggtt
     2401 aattgatcat tttatcggcc caataaccat tgctggcatc gttctgctga tcattgtggc
     2461 caaatttact ggcaatctta agctggggtc aggagaaaaa agcaacagga aaacggggaa
     2521 tcgtaatgct cattctcgct cttcgtcgag aaattctttc cgcggtggtg gcggttcttc
     2581 aggaggcggc ggttcatccg gccgttggtg atcgtaatgc gcttaatctt tattctattc
     2641 actttgtggt gccttcctgg gcttgcccag caaattgctg tgccggagct gcgccaacaa
     2701 gtgactgata ttactggtac gttaagcacg tctgaacaac aatcactgac acagcaactg
     2761 caggatatta cacataaaac acgggcgcag gtcgctgtat tagtagtgcc gtctaccgga
     2821 gacgattcca ttgaacaata tgctacaagg gtttttgata gctggaaact gggagataag
     2881 cagagaaacg atggtatttt acttctggtt gcctgggagg atcatgctgt tcgtattgag
     2941 gttggctacg ggctggaagg ggtggttacc gacctgcaag cggcgaagat tatcagggat
     3001 atattgatcc ctgccttcaa aagtgatgac ctgatgggtg gattaacact ggcaagcgaa
     3061 aatatcggcg cgcttctgtt gaatggtgaa ttaccggaag acagggggga ttattacagt
     3121 atcaatcccc ctattccatt atcgcttgct gtaattatat tgctggcggt actttcatat
     3181 tttattgttt ttaccgatcc gtcgaattta ccctggatca ctctcactgg cgccatttac
     3241 gggatggtat tcctctatgt tgccgagcca ggaccatgga ccaatttaat cgttgcctgc
     3301 ggtatgttaa cgccttttgc gatcgtacca ctggttattt tttggcttat cgtcaataaa
     3361 aagctacgcg ccaaatacaa aaaactcagt aaagaaagag cttcaagaaa aggttcttct
     3421 tcatcctctt ccggtggcgg ctcaagtggc ggaggctcag gcggcgggtt tagcggcggt
     3481 ggcggttctt ctggaggtgg cggcgcgtcc ggtcgctggt aactcaacca taaaaaacgc
     3541 cagcccgaag gctggcattt ttaaatcaga taaagtcaga cttatgcctg gcctttgatc
     3601 tctttacgac cgttgtacgg tgctttttcg cccagagctt cttcgatacg aatcagctgg
     3661 ttgtatttag caacgcggtc agaacggctc atagaaccag ttttgatctg gcctgcagca
     3721 gtaccaacag ccaggtcagc gatggtagcg tcttcagttt cgccagaacg gtgagagata
     3781 actgcagtgt agccagcgtc tttcgccatc ttgattgcag ccagagtttc ggtcagagaa
     3841 ccgatctggt tgaatttgat caggatggag ttagcgatac ctttttcgat accttctttc
     3901 aggatcttgg tgttggttac gaacaggtcg tcaccaacca gctggatttt gtcgcccaga
     3961 actttggtct ggtatgcgaa accatcccag tcagattcgt ccagaccgtc ttcgatggaa
     4021 acgatcgggt actgtttggt cagttcttcc aggaagtgag tgaattcttc agaggtgaat
     4081 gctttgttgc cttcgccagc cagaacgtat ttaccgtctt tgtagaattc agaagctgcg
     4141 cagtccatcg ccagagtgat gtctttgccc agttcataac cagcagcttt aacagcttca
     4201 gcgataacag ccagagcttc agcgttggaa cccaggttcg gcgcatagcc accttcgtca
     4261 ccaacagcag tgttcatgcc tttcgctttc agaacttttg ccaggtgatg gaaaacttca
     4321 gaacccatgc ggatggcttc tttcacagtt ttcgcgccaa ccggctgaat catgaattcc
     4381 tggatatcaa cgttgttgtc agcgtgctca ccaccgttga tgatgttcat catcggaacc
     4441 ggcatggagt atttgcccgg agtgccgttc agttcagcga tgtgctcgta cagcggcata
     4501 cctttagcag ctgcagcagc tttggcgtta gccagagata cagccaggat tgcgttcgcg
     4561 ccgaatttgg atttgttttc ggtgccgtcc aggtcgatca tgatcttgtc aatgccagcc
     4621 tgatctttag catctttgcc aatcagcgcc tgagcgatcg ggccgtttac cgcagcaaca
     4681 gctttggtta cgcctttacc caggaaacgg gatttgtcgc catcgcgcag ttccagagct
     4741 tcacgggaac cagtagaagc acctgacgga gcagctgcca taccgacgaa accaccctcc
     4801 agatgtactt cggcttcaac agtcgggtta ccacgggagt cgatgatttc acgaccgatg
     4861 atttttacga ttttggacat taggttttcc tcaagtcact agttaaactg aaactccaga
     4921 caaacaacgc gtacccaggg tacgcgttgc cgttctaact tttttactta cttcgcctga
     4981 cgtttctgga actcgctggc ggctttcaca aagcctgcaa acagcgggtg accatcacgt
     5041 ggagtagaag taaactccgg atggaactgg caagccacga accacgggtg attcggaact
     5101 tcgatgatct cgaccaactg atcatccccg gaacggcccg caacgcgcag acctgcatct
     5161 tcaatctgtt tcaacagcat gttgttgact tcgtaacggt gacgatgacg ctcaacaatt
     5221 gtcggcgcat tgtacagctg gcgaaccagg ctatcgtcaa ccaactggca ctgctgtgcg
     5281 ccgagacgca tggtaccgcc gagatcgctc ttctcgctac gaacttcaac gttgccgttt
     5341 tcatcgcgcc actcggtaat cagcgccaca accgggtact tacagtctgg cacaaattcc
     5401 gtagagttgg cgttctccat gttggcaaca tggcgagcgt aatcaattaa cgccacctgc
     5461 atacccaggc aaatgcccag ataaggaata ttgttctcac gcgcaaaacg cgcggtcgta
     5521 atcatgcctt ctacgccacg atagccgaaa ccgccaggta cgaggattgc gtccagacct
     5581 ttaaggattt caacgccgcg cgtttcaaca tcttgtgaat cgatcagttt gatgttgacg
     5641 ctgacacgat tcttcagccc accgtgtttc agtgcttcga tcactgattt ataagcatcc
     5701 ggcagttcaa tgtacttgcc gaccataccg atggtgactt cacttaccgg attcgcttct
     5761 tcgaagataa cctgttccca ttcggacaga ttcgcttccg ggcagtttaa gctgaatcgt
     5821 ttacaaatat aatcgtccag cccctgagat ttcaacaggc ccggaatttt atagatggaa
     5881 tcgacgtctt tcagagaaat aaccgctttt tccggaacat tacagaacaa tgcaatcttc
     5941 gcacgttcgt tcgccggaac ggcgcgatct gaacgacaaa tcaggatatc aggctggata
     6001 ccgatggaga gcagctcttt tacagagtgc tgagtcggtt tggttttgac ttcaccagac
     6061 gccgccatgt acggcaccag cgtcaggtgc ataaacaggg tgtgctcacg gccaatttca
     6121 acagccatct ggcgaatcgc ttcgaggaac ggcagggatt cgatatcacc tacagtaccg
     6181 ccgatttcga ccagtactac gtcatgacct tcgccgcctt ccagcacacg ctctttgata
     6241 gcgtttgtga tgtgcggaat aacctgtacg gttgcgccga ggtagtcgcc gcggcgttct
     6301 ttacgcagaa cgtcagagta gatacgaccc gtggtgaagt tgttgcggcg gctcattttg
     6361 gtacgaatga aacgctcgta gtgccccagg tccaggtcgg tttcagcgcc gtcttcagta
     6421 acgaacactt ccccgtgttg gattgggctc atagtacctg gatcgacgtt gatgtacgga
     6481 tccagtttca tgatggtcac attgaggcca cgggcttcaa gaatggctgc gagggaggct
     6541 gcggcaatgc ctttacccag agaggatacg accccgccgg tcacaaaaat atagttcgtt
     6601 gtcatgctga acctgagaag ttaggttgaa aagacgatgg aataaccagg acgggaaagc
     6661 agtatacccg aacatgacct gtgccacaaa ctttcattat ccctcctctt cgccagcgca
     6721 ctattgaaat caggagtgag aaaatagccc ctttggggta aatgtttttg acgcaaatca
     6781 agcgcttgtc atttaaaaaa tgacacaaat ggcgcttgac cgcgtaattc ccttagagat
     6841 caatttcctg ccgttttacc tgttgccaga cttcttccat tgtttcgagg tcaacacctg
     6901 tcatttccag tccacgcgcg gcaacaatac gctccacttc gcgaaaacga agctcgaatt
     6961 tttcgttcgc tttttgcaat gcgatttccg cttttgtccc taaatgacga gccagattga
     7021 ccgtggcaaa cagcaggtcc cccatttcct cctccagttt agcctggtcg acaacagcct
     7081 gccgcgcttc gtacatcacc tcgtcgatct cttcgtagac tttatcgact accggaccaa
     7141 gcgtcgtcca atcgaagcca acgttggcgc aacgtttctg gattttttgc gcacgcatta
     7201 aagccggtaa actacgagga atatcgtcca gcgccgaatg ctgcgctttc tgcgcgcgct
     7261 cttcggtttt gatctcctcc caacgggcga gaacttcgcg gctgtttccg gcagaaccgt
     7321 cagcaaaaac atgtggatga cggcgttcta atttatcgct aatcgcggcg caaatatcat
     7381 taaaatcaaa gcgcccttct tcctgggcca tttgtgcgta aaacaccacc tggaacagca
     7441 gatcgccgag ttcaccgcgc agatcgtcaa agtcttcgcg ggcaatagcg tccagcacct
     7501 cgtaggtttc ttcaagggtg taaggcgcaa tagaagaaaa tgtctgctct ttatcccacg
     7561 ggcagccgtt ttccgggtcg cgcaggcgct gcataatagt gagcaaacgg tcgatttgat
     7621 tcattagaat gtcctgaaaa ttgcgggtct gtcaggtgga aacctgtgac cagaatagaa
     7681 gtgagttagt aacacctacc caatcagtac gttaattttg gctttaatga gttgtaattc
     7741 ctctggggca accgttcctt tcttcgttgc tccttttgcc cgccaggcga tactttttac
     7801 ctgatcagct aacgctacgc catcacgttc ctgaccggat aaaacaactt cgaacggata
     7861 tccttttgat tgcgttgtac aaggaacaca cagacacata cctgttttgt tgttgtacat
     7921 gaacggactc aggacaacag ccggacgatg tccggcttgc tcgctacctt ttgtcgggtc
     7981 aaaatcaacc caaatcagat cgcccatatc gggtacgtat cggcttacca ttaccagact
     8041 tccttatctt tcggctctcc ccagtcgata ttctcgtgga ggttttccgg cgtgatgtcg
     8101 ttgaccagtt cagcaagcgt aaatacgggc tctttacgca ctggctcaat aattaatttg
     8161 ccatccacca ggtcaatctt cacttcatca tcaatattca gattgagcgc ctgcattaac
     8221 gtagccggga tccgcaccgc cggtgaattt ccccaacgct ttatgctact gtggatcata
     8281 accctttcct caaaccgcta tcatatatag atacagtata tatcaatcta cattgtagat
     8341 acgagcaagt ttcggcctaa ctcccgtgca accgacgcgc gtcgataaca tccggcacct
     8401 ggttgagttt acccagcacg cgccccagca cttgcaggtt gtaaatctca atggtcatgt
     8461 cgatggtcgc cagttgctgt ttggtgtcgc tacggctggc aacgccaagc acgttcacct
     8521 tctcgttggc gagaatggtc gtgatatcac gtaacaaccc actacgatca ttagccacca
     8581 cgcggaccac cagcgaatat ccggcggagt agctctcgcc ccataccgcg tcaacaatgc
     8641 gttctggcgc atgggagcgc agttccgcca gttgttcgca atcggcgcgg tgtactgaaa
     8701 taccgcgtcc ctgggtaatg aagccgacaa tctcatctcc aggaatcggc tggcagcagc
     8761 gcgcgatgtg gtgcatcagg ttaccgacac cttcaaccac cacacgaccg ttatctttac
     8821 tgcggttttg cggtgtgtag cttttttgct gaagctgttt cagcgcggcg gcgtcctgct
     8881 cttcggcact cggcttatta aattgcgatt gcaggaagtt caccatctga ttgagacgga
     8941 tatccccgcc accaatcgcc gccagcaact cgtctacatc attgaagttg taacgcggca
     9001 gcagatgttt ttctgcttct ttcaggctga tccccagatg ttccagctcg tcgtcaagga
     9061 tttgccgccc agccagaatg tttttgtcac ggtcctgttt acggaaccag gcgtgaattt
     9121 tcgaacgccc acggctggtt gtgacgtaac cgaggtttgg gttcaaccag tcacggctgg
     9181 ggttcggctg tttctgggtg ataatttcaa tctggtcgcc catctgcagc tggtaggtga
     9241 acggcacaat gcgcccgcca atttttgccc cgatgcagcg gtgtccgaca tcactgtgga
     9301 tgtggtaagc gaagtccagc ggcgttgatc ccgcaggcaa atcaacgaca tcacctttcg
     9361 gcgtaaagac atacacccgg tcgtcaaaca cctgactacg tacttcgtcg agcatttcgc
     9421 cggaatcagc catctcttcc tgccacgcaa tcagtttacg cagccaggca atccggtctt
     9481 catgtcccga acgtgcgccg ccagcagccg cgccctcttt atatttccag tgcgcagcaa
     9541 cacccaactc tgcatcttca tgcatctgtt tggtgcggat ttggatctca acggtttttc
     9601 cacccggccc cagaaccacg gtatgaatag actgataacc gtttggtttc gggttagcga
     9661 cgtaatcgtc aaactcatcc ggcaggtggc gatagtgagt gtgcactatc cccagtgcgg
     9721 cataacaatc ctgtaaacgc tcggcgacaa tacgtaccgc acgcacatca aacagctcat
     9781 cgaaggcgag gttctttttc tgcattttgc gccagatgct gtagatgtgt ttcggacgac
     9841 catacacttc agctttaacg ccttcagctt tcatctcagc gcgcagatga ccgacaaact
     9901 cttcgatata gtgttcgcgg tcgagacggc gttcatgcaa cagttttgcg atgcgtttgt
     9961 attcggtcgg gtggagataa cggaagcagt aatcttccag ctcccatttc agttgcccaa
    10021 tcccaagacg gttcgccagc ggcgcgtaga tattggtgca ctcttttgcc gccagtacgc
    10081 gttcatcttc cggcgcatct tttacttcgc gcagatgagc aatacgctcc gccagtttga
    10141 tgaccacgca gcgaaaatca tcgaccatcg ccaataacat ccggcgaacg ttatcgacct
    10201 gttcggagga aacagaatca gtgtgcgtcg ctttcagctg gcggatcgcc gccatatcac
    10261 gcacgccgtg aataaggtta acgaccgact taccgacgct ctcacgcagc acatcttcgc
    10321 tgactacgtt ggcatccgcc agagggaaca gcagcgccgc ccgcagtgtg tcaatgtcca
    10381 tacttaatgt cgagaggatc tccaccatct caacaccacg ccacaataat agactggcat
    10441 ccggatgccc ctgcgtctgc tgcagacaat acgcccaggt ttcggctaag cactcacacg
    10501 acttctggct ggtaataccc agacttgcga tccatttttc cggatcaaat tcaccagcct
    10561 tattgatatg tgcacttctt accgcaacca tcgtcctctc ctttagggac cagacctgcc
    10621 gaaatcggca aatcgcaact atttaacgcg cgagaaaagt accatcgatt ccagatgtcc
    10681 cgtgtgtggg aacatatcca gcatcgccag tcgcgcaatg gtatatcctg cttttaataa
    10741 cgcttcgcta tcccgagcca gcgttgcagg gttacaggat acataaacta tacgaatagg
    10801 ttccagtttt ataatttgct gcataacacc tgcggcacct gctcgcgccg ggtccagcaa
    10861 cactttatcg aagccgtttt tcgcccacgg ctgctttgtg acatcttctt caagattttc
    10921 gtgataaaac gtcacattct gtaagccatt aagacgcgca ttctgctggc ctttttccac
    10981 cagcgccgga acaccttcta cacccaccac actggcagct tgtgtcgcca atggcagtgt
    11041 aaagttgccc ataccgcaga acagatccag tacgcgatct tcaggttcca catccagcca
    11101 ttccaacgca cgcgctacca ttttttggtt cacacccgca ttgacctgaa taaaatcgcg
    11161 cgggctaaaa gttaagcgca acccgtttga gtcataccag ggcatctcac cagagacggt
    11221 ttcgagtatc tcactatcgg gggcgagata cagatccagg ccttcagaat gcgaaaagcg
    11281 ttccagtttt tcgcgatctg ccgaacttag cggtgcggta tggcgcaaaa tcatcagcgt
    11341 gccgctggtt gcctgtacca gttcaacatg accaagatgg cgcatagctt gtaagctgcc
    11401 caggcatgcc ctgactttgg gcagcaatgc ttcaagttgg ggcactaaaa tggggcattg
    11461 tttaacgtcg acaatgtcac tggagcccgc tttgcgaaac cccatctgaa gttgctgtgt
    11521 tttcggtaag taattcagac ttaaacgcgc gcgacggcga tagccccagg gaacatcggc
    11581 gatcacttca gagacttcgt gtttcattaa tcgggcgagt gccgcacttt tgcttcgctg
    11641 ctgtaaatcc acgctggcgt gttgttgctg acagccgccg catacgccaa aatgaggaca
    11701 gcgtggcgtt tcgcgttccg ggctatcgct taaccggcgt acgactttag cgcgggaata
    11761 ctgtttttta tcttcagtaa cagtaacttc cgcgttttcc tgcggtaata atccggggat
    11821 aaatagcgtt ttgccgttat gtcgcgccac gccctgacca aaagagtcga ggtcgttgac
    11881 tgaaacggtt atgatctgac gcgtcgtcgt gcgtcgtttt gcagagtaga attgcgccat
    11941 tggcgagact ttctcaattt aacagtgtga ccttaattgt cccataacgg aactccatga
    12001 ccaactacag cctgcgcgca cgcatgatga ttctgatcct ggcaccgacc gtccttattg
    12061 gtttattgct gagtatcttt ttcgtcgtgc atcgctataa cgacttgcag cgtcaactgg
    12121 aagatgccgg tgccagcatt attgagccgc ttgcagtttc tactgaatat ggcatgagcc
    12181 tgcaaaatcg cgaatctatc ggtcagttaa taagcgtact gcatcgtcgc cattccgata
    12241 ttgttcgcgc gatttcggtt tatgatgaaa ataaccgact ctttgtcacc tccaattttc
    12301 atcttgatcc ctcatcaatg cagctcggca gcaacgtgcc gtttcctcgc cagctcactg
    12361 tcactcgtga cggcgatatt atgatcctgc gcacgccgat tatttctgag agttactccc
    12421 ccgacgaatc gcccagtagc gatgccaaaa atagtcaaaa tatgttggga tatattgcgc
    12481 ttgagctgga tcttaaatcg gttcgcttgc agcaatataa agagatcttt atttccagcg
    12541 tgatgatgct gttttgtatc ggtattgcgc ttatttttgg ctggcgctta atgcgcgatg
    12601 taaccggtcc gattcgcaac atggtgaata ccgtcgaccg catccgtcgc gggcaactcg
    12661 acagccgggt ggaaggattt atgctcggcg agctggatat gctgaaaaac ggtatcaact
    12721 cgatggcaat gtcgctggct gcttatcacg aagagatgca gcacaatatc gaccaggcga
    12781 cgtccgatct gcgtgaaacg ctggagcaga tggaaattca gaacgttgag ttggatctgg
    12841 cgaaaaagcg cgcccaggaa gcggcacgta ttaaatccga gtttctggca aatatgtcac
    12901 acgagctgcg tacaccactg aatggtgtta ttggctttac ccgcctgacg ctgaaaacag
    12961 aattaacacc aacgcagcgc gatcacctga atacgattga acgttcggca aataatttgc
    13021 tggcaattat taatgatgtt ctcgacttct cgaaactgga agcaggtaag ctgattctgg
    13081 aaagtattcc attcccacta cgcagcacgc tggatgaagt cgttactctg ctggcacatt
    13141 cttctcacga taaagggctg gaactgacgc tcaatattaa aagcgacgtg cctgataacg
    13201 tgatcggcga cccactgcga ttacagcaaa tcatcactaa cctggtgggg aatgcaatta
    13261 aattcaccga gaatggcaac attgatattc tggtagaaaa acgtgcgctg agtaatacca
    13321 aagtgcagat tgaagtgcag attcgggata ccggcattgg tattcctgaa cgcgatcaat
    13381 cgcgcttatt ccaggccttc cgacaggctg atgccagtat ttcccgccgt catggtggca
    13441 ccgggctggg gctggtgatt acacaaaaac tggttaatga aatgggcggc gatatttcgt
    13501 tccatagcca gccgaatcgc ggatcaactt tctggttcca cattaatctc gatctgaacc
    13561 cgaacattat tattgaaggg ccatccaccc agtgcctcgc cggtaaacgc ctggcctatg
    13621 tcgaaccaaa ctccgcagca gcgcaatgca cgctggatat tttaagtgaa acgccgctgg
    13681 aagtggttta tagcccaacg ttctccgcgc tgcctcccgc gcattacgac atgatgttgt
    13741 taggcatcgc ggtgaccttc cgcgagccgc taacaatgca acatgagcga ttagcgaaag
    13801 cggtatcgat gaccgatttc ctgatgctgg cacttccttg ccatgcacaa gtcaatgctg
    13861 aaaaactcaa gcaagatggt atcggcgcgt gtctgctgaa accattaaca cctacgcgcc
    13921 tgttgcctgc cctgacggaa ttttgtcatc acaaacaaaa cacgcttttg cctgtaaccg
    13981 atgaaagtaa gctggcaatg acagtcatgg cggttgatga caaccccgct aacctgaaac
    14041 ttatcggcgc attgctggaa gatatggtgc aacatgtgga actttgcgat agcgggcatc
    14101 aggcggttga acgggcgaaa cagatgccgt tcgatttgat cttaatggat attcaaatgc
    14161 ctgacatgga tggcattcgg gcctgcgagc tcatccacca gctcccccat cagcaacaaa
    14221 cgccggttat cgcggtaacg gcgcatgcaa tggccgggca aaaagagaag ctgcttggcg
    14281 cagggatgag cgattatctg gcgaaaccga ttgaagaaga gcgattgcat aatttgttgt
    14341 tgcgctacaa gcctggcagc ggtatttcgt ctcgcgtcgt gacgcccgaa gtcaacgaaa
    14401 ttgtggtgaa cccgaatgcg accctcgact ggcaactggc actacgccag gcagcaggaa
    14461 aaaccgattt agcgcgcgat atgctgcaaa tgttactcga tttcctgcct gaagttcgca
    14521 acaaagttga ggaacagctg gttggagaaa acccggaagg cctggttgat ttgattcata
    14581 aactgcatgg cagttgcggc tatagcggtg tgccgcgtat gaagaatctc tgccaactga
    14641 tcgaacaaca gctacgtagc ggtactaaag aagaagattt ggagccggag ctgctggaac
    14701 tgttggacga gatggataat gtcgcgcgcg aagccagcaa aattctcggg taacggacgt
    14761 cttgtcgagt agcaatcggc tttttatgac gctggtttca gattggtgac aaagtgcctg
    14821 tgtttatgcc ggatgcggcg ttaacgcctt atccggccta caaaaccaat taaattcaat
    14881 gaattaaaaa atgatgtaga cctgatacat agcacaacag gctattttgc gtttagcatt
    14941 agtctcaaac cggctccaga tagagccggt tttggttttc tgtcttaacg caccatgcac
    15001 gggcgcttgt tatcgaacgt ccagccagga atcagatact gcattcccat cgcatcgtca
    15061 cgcgcgccaa gcccgtgttt ctgatacagc tcatgggctt tcatcacttg atccatatcg
    15121 atttctacac ccagccccgg tttttctggc acctgtacca gcccgccttt gatctcaaac
    15181 ggttctttgg tcaggcgctg attgccttcc tgccaaatcc agtgcgtatc aatagcagta
    15241 attttacccg gtgcagcggc ggcaacatgg gtaaacatcg ccagggaaat atcgaagtgg
    15301 ttgttagagt gcgaacccca ggtcaggcca aattcatggc acatttgcgc cacacgtacc
    15361 gaaccttgca tcgtccagaa atgcggatcc gccagcggga tatcaacgga ttgtaacgag
    15421 agcgtatggc ccatctgccg ccagtcggtg gcgatcatat tggttgcagt cggcaggcca
    15481 gtcgcgcgac ggaactctgc catcacttca cgcccggaga aaccttgctc cgcaccacac
    15541 ggatcttctg cataagccag cgaacctttc aggtatttac caattttaat cgcttcgttc
    15601 agcgaccagg caccgttagg atcgagcgta atacgcgcct gcgggaagcg ttgcgccagt
    15661 gccacaatag actcggcctc ttcttccccg gccagtacac cgcctttcag tttgaaatcg
    15721 ttgaagccat atttttcata cgccgcttcc gccaggcgca ccaccgcatc cggcgtcatc
    15781 gcttcttcat gacgcaggcg ataccagtcg catgagtcat ccggctggct ttgatacggc
    15841 agaggtgtgg ctttgcgatt accgacgaag aacagataac cgagcatttc gacttcgcta
    15901 cgctgttgac catcgcccag cagggatgcc acgttaaccc ccagatgctg ccccagcaga
    15961 tccagcattg ccgcttctat cccggtaact acatgaatag tggtacgcaa atcaaatgtc
    16021 tgcaaaccgc gcccgccggc atcacgatcg gcaaaagtat tacgcaccag cgtcagaacg
    16081 tttttgtatt cacccagcgt tttacccact accagcggaa ttgcatcttc cagcgtttta
    16141 cggattttct cgccgccggg aatttcccct acgccagtgt gaccagaatt atctttgata
    16201 atcacaatat tacgcgtgaa gaacggtgcg tgtgcaccac tcagattcat cagcatactg
    16261 tcatggcccg ccaccgggat gacctgcatt tcagtaacaa caggcgtcgt aaattgagaa
    16321 ctcatattta tgtccttttt cagaatcaat gacggccgaa aacgggacgt ttacggtcaa
    16381 aggtccagcc ggggatcagg tactgcatcg ggcctgcgtc gttacgcgca ccgccaggca
    16441 gacgtttata ggcctcatgt gccttttgta cctgttccca gtccagttcc acgcccagac
    16501 cgggcgcatc aggaacggca atttttccgt ttttaatctc cagcggattt tgggtcaggc
    16561 gacaatcgcc ctcctgccaa atccagtggg tatcgatagc ggtaggatta cccggtgccg
    16621 ccgcgcccac atgggtaaac atcgccagag agatatcgaa atggttgttg gaatggcagc
    16681 cccaggtcag cccccagtcg tcgcaaagct gcgccactcg gactgcaccg gaaagcgtcc
    16741 agaagtgcgg atcggcaagt ggaatatcta ccgcattgag catcaccgca tgacccattt
    16801 cgcgccagtt ggtggcgatc atattagtcg cgacgggcaa gccggtcgcc cgccgaaact
    16861 ccgccatcac ttcacgtccg gagaagccct gttctgcgcc gcatggatct tcggcatagg
    16921 taagaacatc attcagccct ttgcacagag aaatggcttc atcaagcagc caggcaccgt
    16981 tgggatcaac ggtaatccgc gcatccggga agcttttctt caatgcacga acagtgtcga
    17041 tttcttgctc gccaggtaac accccgccct taagtttgaa atctttaaag ccatagcgat
    17101 cctgtgaggc ttccgccaga cgcacaacgg cttcgctgtt catcgctttc tgatgtcgca
    17161 actgatacca ctcatgattg cccggcgtat tttccagata aggaagatcg gttttagtcc
    17221 gatcaccgat ataaaacaga taaccgagga cggtgatagc atcgcgttgc ttgcctggcc
    17281 ctaacagttc gcagaccgga acattcagcg ccttacctag caggtcaagc aaggcggctt
    17341 ccagcgccgc cacggcgtta acgcgcaatt caaaggtcca ggccccttta ccgaaggtat
    17401 caaaatcggc ggcctgatta cctttatgca cctgctgaac cactttattc aggcgcgcaa
    17461 cttcctggcc cagcaccatc ggaatagctt cgaccagtgt ttgataaatc acctctccgc
    17521 ccggcgcttc accaatgccg gtatgcccgg cgttatcggt gagtaccaca atattgcggg
    17581 tgaaatatgc gttatgtgcc ccaccaatat taagcaacat gctatcgtgc ccggcgaccg
    17641 gaatgacctt catatcagta ataacaggac tggattgtgt cgtcatctta ttgccccgcg
    17701 acaggtttca gctcgatacg cttgatatcg cccaccagca ccaggtagct cagtaccgcg
    17761 attaaggcat gaacaccaac ataaatcagc gccccattaa aggagccagt cgtgccaacg
    17821 atataaccaa ttgcgattgg cgtgacgatg ccagaaatgt taccgaacat gttgaacagg
    17881 ccaccggaaa gaccgctgat ctcttttggc gcggtatctg ccattactgc ccaacccagc
    17941 gccccaatgc ccttaccaaa gaaggccagc gccataaagc cgatgatcat ccactcaacg
    18001 ttgacgtagt tgcagaacac catcaccatc gacagcaaca tgcccattac gatcggtgtt
    18061 ttacgcgcaa tgttcagcga tcccgtgcgg cgcatcagcc agtcggaaat aatcccaccc
    18121 agcacaccgc cgataaaacc gcaaaccgcc ggaacggaag ccacaaagcc cgctttcaga
    18181 atcgacatcc cacgcgcctg caccagataa accggaaacc aggtaatgaa aaagtaagtc
    18241 agggcgttga tacagtactg accgatataa acgccgatca tcatccgtga cccgagcagc
    18301 tgtttgatct gcccccactt cacgctgaac ggcactttaa ctttggtgtt ttgctgatcc
    18361 atattgatca gcgcgccacc cgcggcgatg tactccagct ctttctgatt tacccccgga
    18421 tgttgatttg gctcgtggat gactttcaac cagataaagc tgatgacaat gcccaaaccg
    18481 cccataaaga agaagacgtg tgaccagccc acttcatgcg tcagccagcc cataatcggc
    18541 gcgaagatca ccgttgcgaa gtattgagcg gagttaaaaa tcgacaccgc cgttcccctt
    18601 tcctgcgccg gaaaccaggc cgcaacaatg cgactgttgc cggggaaaga tggcgcttca
    18661 gcaagcccga ccaggaagcg cagagtaaac agggcaacga taatgccgaa tccactaaag
    18721 atatcgacga agccttgcag caaggtaaac atcgaccaga taaagatcga ccagaagtag
    18781 acgcgttttg aaccaaaacg atccagcaac caaccaccag ggatctgccc gataacataa
    18841 gcccatgaga aggcagagaa cacatagccc attcccacgg gatcaaggcc gatatctttg
    18901 gccatttccg aaccggcaat agaaagcgta gcgcggtcgc cgtagttgaa ggatgtgacg
    18961 ataaacaaca tcaccactat ccagtaacga gcattcgtgc gtttttccac actgctcgca
    19021 gcctgactta aagaactcat tgttgcactc ctgaaaattc gcgttagcca cgctcactct
    19081 ggactgcgac atcgccagga aatcagaggt gacgtagggt gttttttgcc gtttttatag
    19141 gtcgttcgcc gaatacggcg cgtgtttata tctggcaata gcagtataaa aagcgcgcca
    19201 tagcggctca ccgtgcaaca acacaacatt aatgcgttca atgaggcccg attttggcat
    19261 tagccctgga cggtggaatc cacttcacgg aaatgaaaac aagaacaaga aaggaagggt
    19321 taaaacgaag aaataaagaa gagtatgaaa tggatcgctt gactccaggc aaacgccagt
    19381 aaaaatccgc gctatgaagc agtttttact ggcatttgcc tgaaaagatt cgattcagca
    19441 ccgctaaaac gacatttacc gctcgctgaa catatcagga caacagcgtg ccccactgtt
    19501 cgacccacgg atttgattcc gtttccggtt ccgggttctc actggcgtca atcaacagca
    19561 tttcgccaac ccgctgagcg ctctgttcct gcaacaaggc attgaactgt ttgccgccat
    19621 tgcagaaatt tacataactg ctgtcgccga gcgcaatcac gccataacgc agattcggct
    19681 ggaagcccag actatctttg attccctgaa agagcggtac aatgctatca ggaaggtcgc
    19741 cctgcccggt cgtggacgta accaccagaa catacttatc ctgatagggc agccagtcgc
    19801 ttaattcagg atcttcaaat accgttgctt tgtggccctg tgtggtcaga attgcttctg
    19861 cttcttccgc cactaacagc gaatttccgt acatggtgcc gacaaaaata ccaatttccg
    19921 ccataccgtt ttctccctgg attaggaact tatctctcca tcctgacccg atggcgcact
    19981 gaactcaacc ctttcatttt caggaagcag accgcgccag ccaaattgtg ataacgcctg
    20041 catccaggtg tcgtccaggc ccgcgtgaat agtcagcggc tcgccagtaa aaggatgcgt
    20101 caatgacaac tgactggcat gcagcattaa ccgctgaaga ccaaaatgct cagcaccgct
    20161 gcgattctgg cgtaaatcgc catgtttgct atcgccaata atcggatgac gcaaatgggc
    20221 aagatgtcgg cgaagctgat gtttgcgtcc ggttttcggc tccagttcca ccaggccgta
    20281 gcgcgtggtc gggtaacgtc cggtcgctac cggcatttct acggtcgcca gaccgcgata
    20341 atgcgtcact gctggctgcg ggcctttatc ttcgcgggca aatttatcag cgattttgtc
    20401 cagttcttcc accagtggat aatccagcac cgcttcttcc atcaaccagc cgcgcacaat
    20461 cgcatggtaa cgtttctgga tttggtgctg ttcaaactgt tgtgccagca gccgtccggc
    20521 ctcgctggat aatcccatca acaacacacc agaagttggt cggtccagac gatgagcagt
    20581 aaaaacatgc tgtcctatct ggtcacgcac ggtttgcatg accactactt tctcgtcgcg
    20641 atccagccag ctgcggtgaa ccagccagcc ggagggttta tttaccgcaa ccagccattc
    20701 atcctgatag agtatttcca gcattagctc gcatcatccg caaaaagagc atccagtttt
    20761 tccaactcag ccagaataag cgcgcgttgc ggatggtccg tcgccagcgc catttcataa
    20821 tagggagcaa cggcaaaagc gcccggtaac ggctggttgt tatttaacaa atcgtgcatt
    20881 cgcgggatca gcacccactg caaccactcc agtggttcca tggtgtccat aaagaacggt
    20941 tgggtactgt taaattgatg cggctggggt acatcgtttc gccagtgctg atgttcacgc
    21001 agtaacgctt caagcgcctg caactggaga cgaacgcggt catgagtggt cataaagcaa
    21061 cctcaataag aaaaactgcc gcgaaggata gcaggaataa aaaaagggag cactgtattc
    21121 acagtgctcc cggttcgttt cgcagcattc cagctacatt tgttgctccc tgctcatcct
    21181 tgacaacttt tcctctggcc ttgcggccaa tcgttcatcc tgaactattg cttcctgctc
    21241 acaccacccc gatgtgatac ttcatcctga agtgtccctg gccttcctga cccaccgaat
    21301 catcctgacc ggttctcatt ctccatcctg gaggtgtcct ttaacgcgtc ctgcgtcatc
    21361 ctcttcgctt catccagaag cctttccctg aaacaccatc ctggtgtgtc cttcagaagt
    21421 gtcatcatcc tgatgttcac ttcgttgtct gactccctgt cgacgaagat agaatcgtct
    21481 ttttcgcgaa gtcttacaag gcgcttacag acaatgcctt aaggaaaatt tcatatgaaa
    21541 gttaagaatt aaaaagcaac atattgattt taaatgaaaa taacgattct tatcttttta
    21601 agtattcgct tatttcctgg cgatctccta cagagtctgt aagagatctc tcacaaccag
    21661 atggggtatt tggattacag aagaggctta agttgattaa ggaattccgc aagatttgag
    21721 gctaaatgcg tccgtttacg tgttcccagc gtctctttac acacttcacc ggagagatta
    21781 cacaccgaga tgacctctaa ctcttcttcc agcgtcgcga taaatagcgt gggcggcagt
    21841 tttagccttt tctgagtcac cagatgacca atcagatttt cctgcacccg acggaagtcg
    21901 tcttcactcc aggtttgcag caatgtcagt ttgatatcgc caaactgcgc gtgcatatcc
    21961 ccggcaaact gagtcgtata aaaggtatga attgtgggtt gtatcacaat atcaaaagcg
    22021 cgttcaaccg cgtttacatt ttgttccccg gtaaacggct ggggttgcca gtatacggca
    22081 tcttcagtag ttgaaataat gcacggtgaa ggaacgccgt acagttcctc acttaacggc
    22141 cagcttttat gctcttcatg ccacgcatcg cagtagcgtg cagtaaaatc tttcagggct
    22201 tgtgcggtca aatcgtccac taatttctct cttcacttaa accagataca cttgtcgttt
    22261 agtttatctg gtttatgacg gtgaaacatg tcttcttatg caaaccatca ggcacttgcg
    22321 ggcctgactc ttggaaaatc aaccgattac cgggatacct atgacgccag cctactgcaa
    22381 ggcgttccac gcagcctgaa tcgcgacccg ctgggtctga aagcggataa cctgcctttt
    22441 cacggtacgg atatctggac gctgtatgaa ctttcctggc tgaatgcgaa agggttgccg
    22501 caggtcgctg tcggtcatgt tgaacttgat tacaccagcg taaatctgat tgagtcgaag
    22561 agttttaagc tttatctcaa cagttttaac cagacgcgtt ttaataactg ggatgaggtg
    22621 cgccagacgc tggagcgcga cttaagcact tgcgctcagg gtgaggttag cgtggcgtta
    22681 tatcgtcttg atgaactgga aggccagccg ataggtcatt tcaatggcac ttgcattgat
    22741 gaccaggata tcactatcga taactatgaa ttcactactg actatctgga gaatgccacc
    22801 agtggtgaaa aagtagtgga agagacgctt gtcagccacc tgctgaaatc aaactgcctg
    22861 atcacccatc aaccagattg gggttcgatc caaattcagt atcgtggacg ccaaattgac
    22921 agagaaaaac tgctgcgtta cctggtctct ttccgtcatc acaacgagtt ccacgaacag
    22981 tgcgtggaac gcatctttaa tgacctgtta cgcttctgcc agccagagaa attgagcgtt
    23041 tacgcacgtt atacccgtcg tggcggtctg gacattaacc cgtggcgcag taatagcgat
    23101 tttgtcccat cgaccacaag actggttcgg caataaattt tttctcaatt ttgcgtgctg
    23161 gattcacgca gaaggttgtg aaaggtcatc aggcagggct attgtaatca aagggaatga
    23221 cgatattcgt cccataagga gtttttcttg attacacata ttagcccgct tggctccatg
    23281 gatatgttgt cgcagctgga agtggatatg cttaaacgca ccgccagcag cgacctctat
    23341 caactgtttc gcaactgttc acttgccgta ctgaactccg gtagtttgac cgataacagc
    23401 aaagaattgc tgtctcgttt tgaaaatttc gatattaacg tcttgcgccg tgaacgcggc
    23461 gtaaagctgg aactgattaa tcccccggaa gaggcttttg tcgatgggcg aattattcgc
    23521 gctttgcagg ccaacttgtt cgcggttctg cgagacattc tcttcgttta cgggcaaatc
    23581 cataataccg ttcgttttcc caacctgaat ctcgacaact ccgtccacat cactaacctg
    23641 gtcttttcca tcttgcgtaa cgctcgcgcg ctgcatgtgg gtgaagcgcc aaatatggtg
    23701 gtctgctggg gcggtcactc aattaacgaa aatgagtatt tgtatgcccg tcgcgtcgga
    23761 aaccagctgg gcctgcgtga gctgaatatc tgcaccggct gtggtccggg agcgatggaa
    23821 gcgccgatga aaggtgctgc ggtcggacac gcgcagcagc gttacaaaga cagtcgtttt
    23881 attggtatga cagagccgtc gattatcgcc gctgaaccgc ctaacccgct ggtcaacgaa
    23941 ttgatcatca tgccggatat cgaaaaacgt ctggaagcgt ttgtccgtat cgctcacggc
    24001 atcattatct tccctggcgg tgtgggtacg gcagaagagt tgctgtattt gctgggaatt
    24061 ttaatgaacc cggccaacaa agatcaggtt ttaccattga tcctcaccgg cccgaaagag
    24121 agcgccgact acttccgcgt actggacgag tttgtcgtgc atacgctggg tgaaaacgcg
    24181 cgccgccatt accgcatcat cattgatgac gccgctgaag tcgctcgtca gatgaaaaaa
    24241 tcgatgccgc tggtgaaaga aaatcgccgc gacactggtg acgcttacag ctttaactgg
    24301 tcaatgcgca ttgcgccaga tttgcaaatg ccgtttgagc cgtctcacga gaatatggct
    24361 aatctgaagc tttacccgga tcaacctgtt gaagtgctgg ctgccgacct gcgccgtgcg
    24421 ttctccggta ttgtggcggg taacgtaaaa gaagtcggta ttcgcgccat tgaagagttt
    24481 ggtccttaca aaatcaacgg cgataaagag attatgcgtc gtatggacga cctgctacag
    24541 ggttttgttg cccagcatcg tatgaagttg ccaggctcag cctacatccc ttgctacgaa
    24601 atctgcacgt aatctcccct cccccggtga cgtttgttgc cgggttttct cgtttttggt
    24661 cacttactca tcaactcatt tcatttgtta tatgaatgtt tcttaccacc ttcacgcgac
    24721 aaatatcatc acagttaata tgtcatacaa tttatattgc aacgcaaacg tttccctatt
    24781 ttcataaacc gtattttatc gctatgaaaa agaaattatc gccatgatta actaaaagta
    24841 ttgatttttt cagttcaacc tacatatatt gcgcgccccg gaagaagtca gatgtcgttt
    24901 aatgggcaaa tattgccttt aaattctctt ttacttttga tttacagagt aaagcgttgg
    24961 gataatctat cttccaagta gattattgta tttgggatca agatcactga tagatacata
    25021 acttgtgtgt atctttccgc cctcaaatta ttacggcggt aaatgattaa gccatcgccg
    25081 atagacagat ttcattttta cggtcaggca ccttcccggg ctgaactggc taaaagctga
    25141 attatttgca ttcctccagg agaaatagat ggaaacgact caaaccagca cgattgcgtc
    25201 gaaagactct cgtagtgcct ggcgcaagac agacaccatg tggatgctgg gcctttacgg
    25261 cacggcaatc ggcgcgggcg tgctgttcct gccaatcaac gccggtgttg gcggtatgat
    25321 cccgctgatc atcatggcta tccttgcgtt cccgatgacg ttttttgctc accgcggcct
    25381 gactcgcttc gtactgtctg gtaaaaaccc gggcgaagac atcaccgagg ttgtagaaga
    25441 acactttggt attggcgcag gtaaactgat taccctgctc tacttcttcg ctatctaccc
    25501 gatcctgctg gtttatagcg tggcaatcac caatacagtt gaaagcttca tgtctcacca
    25561 gctgggtatg acgccaccgc cgcgtgcgat tctgtcgctg atcctgatcg tgggtatgat
    25621 gaccatcgtt cgcttcggtg agcagatgat cgttaaagcg atgagtattc tggtattccc
    25681 gtttgttggc gtactgatgc tgctggctct gtacctgatc ccgcagtgga acggcgcagc
    25741 actggaaacg ctgtctctgg acactgcatc tgcaaccgga aacggtctgt ggatgaccct
    25801 gtggctggca attccggtaa tggtgttctc gttcaaccac tctccgatca tctcttcttt
    25861 cgccgttgcg aagcgtgaag agtacggcga tatggcagaa cagaaatgtt cgaagatcct
    25921 ggcattcgca cacatcatga tggtgctgac cgtaatgttc ttcgtcttca gctgcgtact
    25981 gagcctgact ccagcagacc tggctgcggc taaagagcag aacatctcga ttctgtctta
    26041 cctggctaac cactttaacg caccggttat cgcgtggatg gctccgatta tcgcgattat
    26101 cgctatcacc aaatccttcc tcggccacta cctgggcgca cgtgaaggct tcaacggtat
    26161 ggtgattaaa tctctgcgtg gtaaaggtaa gtctatcgaa atcaacaagc tgaaccgtat
    26221 cactgcgctg ttcatgctgg taacgacctg gattgttgcc accctgaacc cgagcatcct
    26281 gggtatgatt gaaaccctgg gcggcccaat catcgcgatg atcctgttcc tgatgccgat
    26341 gtacgcaatt cagaaagtac cggcaatgcg taagtacagc ggtcacatca gcaacgtatt
    26401 cgttgtcgtg atgggtctga ttgcaatctc cgcaatcttc tactctctgt tcagctaagt
    26461 cctttcgcgc cgctttcggg cggcgcttcc tccgttttaa cgcgatgtat ttcctatgat
    26521 tagcgtattc gatattttca aaatcggcat tggcccttcc agttctcata ccgttggacc
    26581 aatgaaagcg ggtaaacaat ttaccgacga tctgattgcc cgtaacctgc ttaaagacgt
    26641 gacccgcgtg gtggttgacg tgtacggctc gctctctctg actggtaaag gccaccacac
    26701 tgatatcgcc attattatgg gcctggcggg taacctgccg gataccgtgg atatcgattc
    26761 catccctggt tttattcagg atgtgaatac tcatggtcgc ctgatgctgg caaacggtca
    26821 gcatgaagtg gagttcccgg ttgatcagtg catgaacttc catgcagaca acctttctct
    26881 gcatgaaaac ggtatgcgca ttaccgcgct ggcgggtgat aaagtcgttt acagtcagac
    26941 ttactactct attggcggtg gctttatcgt tgatgaagag cattttggcc agcaggatag
    27001 cgcaccggtt gaagttcctt atccgtatag ttcagcagcc gatctgcaaa aacattgtca
    27061 ggaaaccggg ctgtcactct ctggcctgat gatgaaaaac gagctggcgc tgcacagcaa
    27121 agaagagctg gaacagcacc tggcgaacgt ctgggaagtg atgcgtggcg gtattgagcg
    27181 cggtatttcc accgaaggtg tgttacctgg caaactgcgt gttccacgcc gtgctgcggc
    27241 actccgccgg atgctggtca gccaggataa aaccaccact gacccgatgg cggttgttga
    27301 ctggatcaac atgtttgcac tggcagtgaa cgaagagaac gctgctggcg gtcgcgtggt
    27361 gactgcgccg actaacggtg cgtgcgggat tatcccggca gtgctggcgt actacgataa
    27421 gtttatccgc gaagtgaacg ctaactcact ggctcgttac ctgctggtag ccagcgccat
    27481 tggttctctt tataagatga acgcgtcgat ttctggtgcc gaagtgggtt gccagggtga
    27541 agttggtgtg gcgtgctcaa tggcggcggc tggtctggca gaactgttag gtgcaagccc
    27601 ggcgcaggtg tgcatcgcgg cggaaatcgc catggagcac aacctcggtc tgacgtgtga
    27661 cccggtcgcc ggacaggtac aggtgccatg catcgagcgt aacgccattg cggcagtaaa
    27721 agcggtgaac gccgcacgta tggcgctgcg ccgtaccagc gagccgcgcg tctgcctcga
    27781 taaagttatc gaaaccatgt acgaaacagg taaagatatg aacgccaagt accgcgaaac
    27841 ctctcgcggc ggcctggcaa tgaagatcgt tgcctgcgat taatcgctct ccagaggcct
    27901 cgttttgcga ggcctcttcc cgatttctca tccagccata gcctgttcca gtatcgaatg
    27961 ttacccttat cgcctgatct ttaagggggt tatcgtggct gttcatttgc ttattgtcga
    28021 tgcactgaat cttattcgtc gcattcatgc cgttcagggt tcgccctgtg tcgaaacctg
    28081 ccagcacgcg ctcgatcagc tcattatgca cagccagcca acccacgctg tcgccgtttt
    28141 tgatgatgaa aaccgcagta gcggctggcg tcatcagcgt ttaccagatt acaaagcggg
    28201 tcgaccgcca atgccggaag agttgcacga cgagatgcct gcattacgcg ccgcctttga
    28261 gcaacgcggc gtcccgtgct ggtcaaccag cggcaacgaa gccgatgact tagccgccac
    28321 gctggcggtc aaagtgacac aggccgggca tcaggcaacg attgtttcga cagataaagg
    28381 ctactgtcag ttactttcac cgacattacg tattcgtgat tacttccaga aacgttggct
    28441 ggatgcgcca tttatcgata aagaatttgg cgttcaaccg cagcagttgc ccgattactg
    28501 gggacttgcg gggatcagca gttcaaaggt accgggtgtt gcgggaatcg gaccaaaaag
    28561 cgccacacag ctgctggtcg agtttcagag tctggaaggg atatatgaga atctggatgc
    28621 ggttgccgaa aagtggcgca aaaaattaga aacccataaa gagatggcgt ttctgtgccg
    28681 cgatattgcc cgcttacaaa ccgatttgca tatcgatggc aatttacagc aattgcgatt
    28741 ggtacggtaa cggcgagccg gatacgccgc aaacgtcgta tccggcatta tcacatcagc
    28801 gcatttacca ggcggtatgg taaagctcta caatatcctc aagcgttgct tcacgcgggt
    28861 tgccaccggt acaaacatca tccagtgccg cctgcgccag tgccggaatg tcttccttgc
    28921 gtacaccaac atcacgcaaa tgtggcggaa taccgacatc acggttgaga gcaaacaccg
    28981 cttcaacagc ggcattacgc gcctcttcca ggctcatacc ttccactttc acgcccataa
    29041 cgcgcgcgat atcgcggtac ttctcaccgg taaagtcagc gttatagcgc atgacatgcg
    29101 gtaacaggat ggcgttcgca acaccgtgtg gagtgttgta aaacgcgccc agtggatgcg
    29161 ccataccatg caccaaccct aacccaacat tcgagaagcc catacccgca acatactgcc
    29221 cgagcgccat ttcttctccg gcatccttat caccagcaac cgatcctcgc agcgccccag
    29281 caatgatttc aatcgcttta atgtgcagtg catcggttag cgcccacgcg ccacgggtaa
    29341 tatacccctc aatagcatga gtgagcgcat cgacacccgt cgcagctttc agcgctggag
    29401 gcataccatc catcatgtca gcgtcaataa acgccacctg cgggatatca tgcggatcaa
    29461 cgcaaacaaa cttgcgccgt ttttcttcgt cagtgatcac gtagttaatg gtcacttctg
    29521 ccgcagtgcc tgctgtggtg gggattgcca gaatcggtac actgggttta ttggtcgggg
    29581 aaagtccttc caggctacgc acatcggcaa actccgggtt gttgctgata atgccaatcg
    29641 ctttacaagt atcctgtgga gaaccaccac caatagcgat caggtaatcc gcgccgctat
    29701 tctggaatac accgagccct tctttgacga cagtaattgt tgggttgggt actacgccgt
    29761 cgtaaatcgc ccatgccagc cctgcagcat ccatcttatg ggtcactttc gccaccacgc
    29821 cacattgcac cagcgtttta tcggtgacga tcagcgcctt ctgataacca cggcgtttca
    29881 cctcatcggt taaagcccca acagcgcccc gaccaaacca tgccgtttcg ttcagaatca
    29941 ttctgttagc catcatcctt ctccttgttg ctttacgaaa ttactcttca atccgtaacc
    30001 cataggtttt gaatttctcc agcactacgg caatttcttc atcgctcagc actggcaccg
    30061 ggtccgtaat cgccagggtc gtcaggtaaa gttgcgccag cacttcaact tcatgcgcca
    30121 gccataacgc tttttccaga ttcacctcac aagcgataag cccatgatgt tgtaacaaag
    30181 ttgccttacg atttttgaga gccagcgcaa catgttcaga aagttcgcgt gttccaaagg
    30241 tcgcataagg cgcgcaagga atagaattac cgccagccgc cgcaatcatg tagtgaatag
    30301 cggggatcgg tcggttaaga atggaaactg ctgtgcaatg aacggcatga ttgtgaacaa
    30361 ccgcgttggc atccggtctg ctttgatagg ccgccatatg gaaacgccat tcgcttgagg
    30421 ggagctttcc ttcctcatgt ttaccgttgc catcaataaa gacaatatgc gactccgtca
    30481 gtttttcata tggaatgcct gtaggcgtaa tcagcatccc atcctgataa cgtacactga
    30541 cgttccccgc tgtcccctgg ttcagtccca ggcgggtcat ttccaggcaa gtgtcaataa
    30601 tctgacgagc aagtttattt cgttccatta gctacctctc tctgattcaa aacagggcaa
    30661 taatgttgtt cctttcacac tattgaatta gccgtttaat tacccaccat cttcttcctg
    30721 attaacaaga aagaaattca caagcttata ttttgtgacc tggttcaact aatcacagta
    30781 aataactgca agttctcttt ttataacccc attaaaaatg accgctctta aaaatattta
    30841 tcaaaacggt catttttcaa ttcctccaag cccggaatga ccgttttcgg cacaaacaat
    30901 taatacggtc atctgatttg tgttttttat gatttatttt ctgaaacggg catgaaattt
    30961 cgattattaa agtgatggta gtcacataaa gtcaccttcc agctaataag tgtgaccgcc
    31021 gtcatattac agagcgtttt ttatttgaaa atgaatccat gagttcattt cagacaggca
    31081 aatattcact gatatgaagc ccgaactcgc tggttttgca cttttgaaaa cataaccgat
    31141 tacgtgctta agcttctgaa cctaagagga tgctatggga aacacatcaa tacaaacgca
    31201 gagttaccgt gcggtagata aagatgcagg gcaaagcaga agttacatta ttccattcgc
    31261 gctgctgtgc tcactgtttt ttctttgggc ggtagccaat aaccttaacg acattttatt
    31321 acctcaattc cagcaggctt ttacgctgac aaatttccag gctggcctga tccaatcggc
    31381 cttttacttt ggttatttca ttatcccaat tcctgctggg atattgatga aaaaactcag
    31441 ttataaagca ggaattatta ccgggttatt tttatatgcc ttgggtgctg cattattctg
    31501 gcccgcggca gaaataatga actacacctt atttttagtt ggcctattta ttattgcagc
    31561 cggattaggt tgtctggaaa ctgccgcaaa cccttttgtt acggtattag ggccggaaag
    31621 cagtggtcac ttccgcttaa atcttgcgca aacatttaac tcatttggcg caattatcgc
    31681 ggttgtcttt gggcaaagtc ttattttgtc taacgtgcca catcaatcgc aagacgttct
    31741 cgataaaatg tctccagagc aattgagtgc gtataaacac agcctggtat tatcggtaca
    31801 gacaccttat atgatcatcg tggctatcgt gttactggtc gccctgctga tcatgctgac
    31861 gaaattcccg gcattgcaga gtgataatca cagtgacgcc aaacaaggat cgttctccgc
    31921 atcgctttct cgcctggcgc gtattcgcca ctggcgctgg gcagtattag cgcaattctg
    31981 ctacgttggc gcacaaacgg cctgctggag ctatttgatt cgctacgctg tagaagaaat
    32041 tccaggtatg actgcaggct ttgccgctaa ctatttaacc ggaaccatgg tgtgcttctt
    32101 tattggtcgt ttcaccggta cctggctcat cagtcgcttc gcaccacaca aagtcctggc
    32161 cgcctacgca ttaatcgcta tggcactgtg cctgatctca gccttcgctg gcggtcatgt
    32221 gggcttaata gccctgactt tatgcagcgc ctttatgtcg attcagtacc caacaatctt
    32281 ctcgctgggc attaagaatc tcggccagga caccaaatac ggttcgtcct tcatcgttat
    32341 gaccatcatt ggcggcggta ttgtcactcc ggtcatgggt tttgtcagtg acgcggcggg
    32401 caacatcccc actgctgaac tgatccccgc actctgcttc gcggtcatct ttatctttgc
    32461 ccgtttccgt tctcaaacgg caactaactg aacatatttt ccgaataaag tgaggaatct
    32521 gtaatgaaaa aaatcagctt accgaaaatt ggtatccgcc cggttattga cggtcgtcgc
    32581 atgggtgttc gtgagtcgct tgaagaacaa acaatgaata tggcgaaagc tacggccgca
    32641 ctgctgaccg agaaactgcg ccatgcctgc ggagctgccg tcgagtgtgt catttccgat
    32701 acctgtatcg cgggtatggc tgaagccgct gcttgcgaag aaaaattcag cagtcagaat
    32761 gtaggcctca ccattacggt aacgccttgc tggtgctatg gcagtgaaac catcgacatg
    32821 gatccaaccc gcccgaaagc catttggggc tttaacggca ctgaacgtcc cggcgctgtt
    32881 tacctggcag cggctctggc agctcacagc cagaaaggca tcccggcatt ctccatttac
    32941 ggtcatgacg ttcaggatgc cgatgacaca tcgattcctg ccgatgttga agaaaaactg
    33001 ctgcgctttg cccgcgccgg tttggccgtc gccagcatga aaggtaaaag ctatctgtcg
    33061 ctgggcggcg tttcgatggg tatcgccggt tctattgttg atcacaactt ctttgaatcc
    33121 tggctgggaa tgaaagtcca ggcggtggat atgaccgaac tgcgtcgccg tatcgatcag
    33181 aagatttacg acgaagccga actggaaatg gcactggcct gggctgataa aaacttccgc
    33241 tatggcgaag atgaaaataa caaacagtat caacgcaatg ccgagcaaag ccgcgcagtt
    33301 ctgcgcgaaa gtttactgat ggcgatgtgt atccgcgaca tgatgcaagg caacagcaaa
    33361 ctggccgata ttggtcgcgt ggaagaatca cttggctaca acgccatcgc tgcgggcttc
    33421 cagggacaac gccactggac cgatcaatat cccaatggcg acaccgccga agcgatcctt
    33481 aacagttcat ttgactggaa tggcgtgcgc gaaccctttg tcgtggcgac cgaaaacgac
    33541 agtcttaacg gcgtggcaat gctaatgggt caccagctca ccggcaccgc tcaggtattt
    33601 gccgatgtgc gtacctactg gtcaccagaa gcaattgagc gtgtaacggg gcataaactg
    33661 gatggactgg cagaacacgg catcatccat ttgatcaact ccggttctgc tgcgctggac
    33721 ggttcctgta aacaacgcga cagcgaaggt aacccgacga tgaagccaca ctgggaaatc
    33781 tctcagcaag aggctgacgc ttgcctcgcc gctaccgaat ggtgcccggc gatccacgaa
    33841 tacttccgtg gcggcggtta ctcttcccgc ttccttaccg aaggcggcgt cccgttcacc
    33901 atgactcgtg tcaacatcat caaaggcctg ggaccggtac tgcaaatcgc ggaaggctgg
    33961 agcgtggaat tgccgaagga tgtgcatgac atcctcaaca aacgcaccaa ctcaacctgg
    34021 ccaaccacct ggtttgcacc gcgcctcacc ggtaaagggc cgtttacgga tgtgtactcg
    34081 gtaatggcga actggggcgc taaccatggg gttctgacca tcggccacgt tggcgcagac
    34141 tttatcactc tcgcctccat gctgcgtatc ccggtatgta tgcacaacgt tgaagagacc
    34201 aaagtgtatc gtccttctgc ctgggctgcg cacggcatgg atattgaagg ccaggattac
    34261 cgcgcttgcc agaactacgg tccgttgtac aagcgttaat acattactcc ctgatgtgat
    34321 gcccggtcgc tccggctacc gggcctgaac aagcaagagt ggttagccgg ataagcaatg
    34381 ttatccggct atattgcagg agcgattatg aaacaagaag ttatcctggt actcgactgt
    34441 ggcgcgacca atgtcagggc catcgcggtt aatcggcagg gaaaaattgt tgcccgcgcc
    34501 tcaacgccta atgccagcga tatcgcgatg gaaaacaaca cctggcacca gtggtcttta
    34561 gacgccattt tgcaacgctt tgctgattgc tgtcggcaaa tcaatagtga actgactgaa
    34621 tgccacatcc gcggtatcgc cgtcaccacc tttggtgtgg atggcgctct ggtagataag
    34681 caaggcaatc tgctctatcc gatcattagc tggaaatgtc cgcgaacagc agcggttatg
    34741 gacaatattg aacggttaat ctccgcacag cggttgcagg ctatttctgg cgtcggagcc
    34801 tttagtttca atacgttata taagttggtg tggttgaaag aaaatcatcc acaactgctg
    34861 gaacgcgcgc acgcctggct ctttatttcg tcgctgatta accaccgttt aaccggcgaa
    34921 ttcactactg atatcacgat ggccggaacc agccagatgc tggatatcca gcaacgcgat
    34981 ttcagtccac aaattttaca agccaccggt attccacgcc gactcttccc tcgtctggtg
    35041 gaagcgggtg aacagattgg tacgctacag aacagcgccg cagcaatgct cggcttaccc
    35101 gttggcatac cggtgatttc cgcaggtcac gatacccagt tcgccctttt tggcgctggt
    35161 gctgaacaaa atgaacccgt gctctcttcc ggtacatggg aaattttaat ggtccgtagc
    35221 gcccaggttg atacttcgct gttaagtcag tacgccggtt ccacctgcga actggatagc
    35281 caggcagggt tgtataaccc aggtatgcaa tggctggcat ccggcgtgct ggaatgggtg
    35341 agaaaactgt tctggacggc tgaaacaccc tggcaaatgt tgattgaaga agctcgtctg
    35401 atcgcgcctg gcgcggatgg cgtaaaaatg cagtgtgatt tattgtcgtg tcagaacgct
    35461 ggctggcaag gagtgacgct taataccacg cgtgggcatt tctatcgcgc ggcgctggaa
    35521 gggttaactg cgcaattaca gcgcaatcta cagatgctgg aaaaaatcgg gcactttaaa
    35581 gcctctgaat tattgttagt cggtggagga agtcgcaaca cattgtggaa tcagattaaa
    35641 gccaatatgc ttgatattcc ggtaaaagtt ctcgacgatg ccgaaacgac cgtcgcagga
    35701 gctgcgctgt tcggttggta tggcgtaggg gaatttaaca gcccggaaga agcccgtgca
    35761 cagattcatt atcagtaccg ttatttctac ccgcaaactg aacctgaatt tatagaggaa
    35821 gtgtgaaatg ctgaaaacaa tttcgccgtt aatttctccc gaactattga aagtgctggc
    35881 agagatggga catggagatg aaattatttt ttccgatgct cactttcccg cccattcgat
    35941 gggaccgcaa gtgatccgcg ctgatggcct gttggtgagc gacttgctcc aggcgattat
    36001 cccgttattt gagctggaca gttatgcacc gccgctggtg atgatggcag cggtagaagg
    36061 tgacactctc gatcctgaag tagaacgacg ttaccgtaat gcgctttcac tacaagcccc
    36121 gtgtcctgac atcatccgca tcaatcgttt tgcgttttat gaacgggcgc aaaaagcctt
    36181 tgcgatcgtt atcacaggcg aacgagcgaa gtacgggaat attcttttaa aaaaaggggt
    36241 aacaccgtaa tctcataccg gtacgcccgc atgacgcggg cggttatcga atgatggggt
    36301 gaaaaatatg aaagcggcac gccagcaagc gatagtcgac ctgctgctga accataccag
    36361 cctgaccacg gaagctctct ctgaacagct aaaggtcagt aaagaaacca ttcgtcgcga
    36421 tctcaatgaa ttacagacgc agggtaaaat tctgcgcaat catggacgcg ctaaatatat
    36481 ccaccgtcaa aatcaagaca gtggcgatcc ctttcacatc aggctgaaaa gccattatgc
    36541 gcataaagca gatatcgcgc gcgaggcgct cgcgtggatt gaagaaggga tggtgatagc
    36601 cttagacgcc agttcaactt gctggtatct ggcacgccag ttgcctgaca tcaacattca
    36661 ggtcttcacc aatagccatc cgatttgcca tgaactcggt aaacgcgaac gcattcaact
    36721 gatcagttcc ggcggcacac ttgagcgcaa atatggctgt tacgtcaatc cctcgctgat
    36781 ttcccaactt aaatcgctgg aaatcgatct gtttattttt tcttgtgaag ggatcgatag
    36841 cagcggcgca ctgtgggact ccaatgccat caacgctgat tacaaatcaa tgctattaaa
    36901 acgcgccgcg caatcgttgt tattgattga taaaagtaaa tttaatcgtt caggggaagc
    36961 ccgcatcggg catctggatg aggtaacgca cattatttct gatgagcgcc aggttgcaac
    37021 ttctttggta acagcctgac ggaaagggta gcaggccgga aacaacgccc cggccttgcc
    37081 tgttatcgct cgtcgcgacg accacccacc gcagcccaga tgcggcggac gtgcaccgtc
    37141 acttcttcgc gatcgtgata caactgccgt gcctgaatct gagcatttat gccatgttca
    37201 tcaagctgtg cctgaatata cgccagattg tgtgacactt cttcgtagcg ttttttcatc
    37261 ggcagtttga ggttgaaaat ggtttcacga caccagccat taaccagcca ctgcgccatc
    37321 aatgccgcaa ctttcgccgg tttttcaacc atatcgcata ccatccagga gatattgctg
    37381 cgcgtcggac ggaatttgaa accgtcttcc cgcagccacg tcacctgtcc ggtatccatc
    37441 agactttgcg ccatcgggcc gttgtcgacg gaataaaccc acatgttgcg cttcaccagt
    37501 tggtaggtcc agccgcctgg gcaagcgccc aaatccaccg cccacatccc gttcgccagg
    37561 cgttcatccc actcatccgc aggaataaac acatgaaatg cctcttccag tttgagcgtg
    37621 gaacgactcg gcgcatctgc cggaaatttc aggcgcggaa tgcccatata gaacggcgaa
    37681 ttattgttgc tgtatgagta accggtatag cagcagcctg gtgcaatgaa gaatacatgc
    37741 actaccggac gcttcggcgt ttcatagttc gccaacaccc ccgcatcgcg caaggcagtg
    37801 cgtagcggca cggtgaattt acggcagaat ttcagcaact ctttgctttc gttggtatcg
    37861 gcaacttcaa cacgcagttc accgcccttc tctactacgc cctgtaacat gccgacaatg
    37921 ggggtaatac gatcttctgg cggcaaatgc tgcaggagtt ccccaaccac aaaccactgg
    37981 cgggcaaaaa ttaatgaact gaacggcagc tcacggatta acttatcgcc atcatcaggt
    38041 tgataacatt cataaatgac ataacccgca ttctctttca cgcgggcaaa accgaaaatt
    38101 tcccgctggc cggctttatc ggtaatttct gcggcgcact ctttttcaaa gcccggacgg
    38161 cacagcaata caaccttatt catgacttac gcccttgcgc tttaaacgga tagcaccaac
    38221 taacattaac gcccagcccg caaggaagct cacgccgcca accggagtga caaacgccca
    38281 caaacgcaga tgggacagtg ccaggcaata aaggctgccg ctgaacagca ccgtgcctaa
    38341 cgcgaggaaa acgctactcc agtaaaacca gatactgatg cgacgctgca ttgccaccgc
    38401 cagacctaag atcgccagcg tatgaaacgc ctggtattcg aggccggtct ggatccagcc
    38461 catctcaacg gcccccatgg ttttacttaa cacatgcgca ccaaaagcgc ccagtgccac
    38521 aaaaatgaag ccgctaatgg cggcgaaaat cagcataaaa cggctggtca tggtcgtacc
    38581 ctacgtaaat tattgttcat aacgaaagcg caatttttct tgttcagcag cggctttcgc
    38641 caggatccat tggcgaaagg cggctatttt acccagttct gcctggctgt catgacaaac
    38701 cagataaaaa gcgtttttac tgaccagaac atcattaaac gggcaaacaa gacgtccggc
    38761 ttcaatttca gattgcgcca tcacgttatt tgccagcgcc actccctgcc cgtggatagc
    38821 cgcttgcagc accatggcgc tatggctaaa aattggccct tgctgaacgt tgatatgatt
    38881 taaccccaat tgacgagtat atgtctgcca gtcacggcgt gacgcatcat gtaatagcgt
    38941 atgtttagcc agatcttccg gggtcttcaa gggtttttcg ccagtcagca gtagcggcga
    39001 acacaccggc aataaatatt cggcgtacag tttttccacc cgtagccccg gccagttgcc
    39061 ccgaccataa aatatcgcca catcaacatc atccgccagc ttatcttcct gacgatcaac
    39121 cgcctggatt cgaacgtcaa ttcccggata agctgaatta aagctggaaa gtcgcggaac
    39181 caaccaatga atggcgaaac tagggagtaa actgaccgtc aacgccccct tggcgctacg
    39241 ggcctggagt ttacgcgtcg cttcggttaa ttgcgaaaat atctctttga tatcgaggaa
    39301 atagctttgc ccttcctcgg tcagcaggag tgaacgatta cggcggcgga acagttttag
    39361 ccccaaaaaa tcctcaagag acttgatttg atgacttact gcggcctggg tcacaaaaag
    39421 ctcttctgct gcgcgagtga aacttaaatg gcgtgctgcg gcatcaaaaa ctcgtaaggc
    39481 atttagcggt ggtaatcgtt tagacatggc ttttaaactt tgatgttaaa tgaatttaac
    39541 aattagatca cactgtgtaa cctattagtt tttttaatct gagccattat aaattgtccg
    39601 ttgagcttct accagcaaat acctatagtg gcggcacttc ctgagccgga acgaaaagtt
    39661 ttatcggaat gcgtgttctg gtgaactttt ggcttacggt tgtgatgttg tgttgttgtg
    39721 tttgcaattg gtctgcgatt cagaccatgg tagcaaagct accttttttc acttcctgta
    39781 catttaccct gtctgtccat agtgattaat gtagcaccgc ctaattgcgg tgcttttttt
    39841 taccttgcga tcgcgaatta ctgatccagt tcgaccatct ctttcacgtc agtacggttg
    39901 atctgctgtt tgttgccatt agcgtcttta tacgaaatca taccggtatc gttatcagtc
    39961 tgtggtttgc cgtcagagac gatggtacgt ccgtcattgg tgtgcatcac atagttcgaa
    40021 ccggaacagg cgctcagggc aaaagtcagc atacaggcag aaataattgc ggcagtcttt
    40081 ttcatcatct tctccttaaa gcgattaata aatcttaata gtattgatta acaggctaaa
    40141 attaacgcct aacactattc agcatatgtt actgacgcgg cttcgccagg atatccagat
    40201 aattctgatg gttagcactc tccttgtatc aaagtgaatt ttgcgtcacg atcggtgcat
    40261 caagccgagg agtaccatga acgtttttaa tcccgcgcag tttcgcgccc agtttcccgc
    40321 tctacaggat gcgggcgtct atctcgacag cgccgcgacc gcgcttaaac ctgaagccgt
    40381 ggttgaagcc acccgacagt tttacagtct gagcgccgga aacgtccatc gcagccagtt
    40441 tgccgaagcc caacgcctga ccgcgcgtta tgaagctgca cgagagaaag tggcgcaatt
    40501 actgaatgca ccggatgata aaactatcgt ctggacgcgc ggcaccactg aatccatcaa
    40561 catggtggca caatgctatg cgcgtccgcg tctgcaaccg ggcgatgaga ttattgtcag
    40621 cgtggcagaa caccacgcta acctcgtccc ctggctgatg gttgcccaac aaactggagc
    40681 caaagtggtg aaattgccgc ttaatgcgca gcgactgccg gatgtcgatt tgttgccaga
    40741 actgattact ccccgtagtc ggattctggc gttgggtcag atgtcgaacg ttactggcgg
    40801 ttgcccggat ctggcgcgag cgattacctt tgctcattca gccgggatgg tggtgatggt
    40861 tgatggtgct cagggggcgg tgcatttccc cgcggatgtt cagcaactgg atatcgattt
    40921 ctatgctttc tctggtcaca aactgtatgg cccgacgggt atcggtgtgc tgtatggcaa
    40981 atcagaactg ctggaagcga tgtcgccctg gctgggtggc ggcaaaatga ttcacgaagt
    41041 gagttttgac ggcttcacga ctcaatctgc gccgtggaaa ctggaagcag gaacgccaaa
    41101 tgtggctggt gtcataggat taagcgcggc gctggaatgg ctggcagatt acgatatcaa
    41161 ccaggccgaa agctggagcc gtagtttagc aacactggcg gaagatgcgc tggcgaaacg
    41221 tccaggcttc cgttcattcc gctgtcagga ttccagccta ctggcctttg attttgctgg
    41281 cgttcatcac agcgatatgg tgacgctgct ggcggagtac ggtattgcct tgcgggccgg
    41341 gcaacattgc gctcagccgc tactggcaga attaggcgta accggcacac tgcgcgcctc
    41401 ttttgcgcca tataatacaa agagtgatgt ggatgcgctg gtgaatgccg ttgaccgcgc
    41461 gctggaatta ttggtggatt aatgacgaac ccgcaattcg ccggacatcc gttcggcaca
    41521 accgtaaccg cagaaacgtt acgcaatacc ttcgcaccgt tgacgcaatg ggaagataaa
    41581 tatcgccagt tgatcatgct ggggaaacag cttccggctt tgccagacga gttaaaagcg
    41641 caggctaaag agattgccgg atgcgaaaac cgcgtctggc tgggatatac cgtggctgaa
    41701 aacggtaaaa tgcacttctt tggcgacagc gaaggacgta tagtgcgcgg cctgctggcg
    41761 gtgctgttga ctgccgttga ggggaaaacc gccgccgaat tgcaggcaca gtcgccgctg
    41821 gcattgtttg atgagctggg attgcgtgcg caacttagcg cctcacgcag ccaggggtta
    41881 aatgcgttaa gcgaggcgat tatcgctgcg acgaagcagg tttaagcaca gaaccgggtc
    41941 ggataagacg tttgcgtcgc atccgacctg attgtttacc cctgacgcgc cgctttcgcc
    42001 atcatcttct tcagcgcatg agaaaccgca acaaaaccaa aggtggcggt caccatcgtt
    42061 gccgcgccaa atccagatgc acaatccatc cgctttggcc cttctgccgt ggctttcata
    42121 gcacaaaccg taccatctga ctgcggatac actagcgctt cagtagagaa cacgcaatcg
    42181 acgccgagct tacctttact gtttttcaca acgccaaaat cgcttttcag gcgctcacgc
    42241 aacttcgccg ccagcgggtc ctgaatcgtt ttcgccagat cggtaacctg aatctgcgtt
    42301 gggtcaatct gcccacccgc gccaccggtc gtgaccagcg ggattttatt gcgccgacaa
    42361 taagcaatca gcgccgcttt gggccgtaca ctatcaatgg catcaatcac gtacgaataa
    42421 cccacgctca tatgctgcgc tacgttatcc ggcgtcacga aatcatccac caccgttaca
    42481 cggcactccg ggttaatctg gcgaatacgc tccgccataa cttccgcttt tgccagccca
    42541 acgttatcgc gcagggcatg aatttgccga ttggtattgg tgacgcacac atcatccata
    42601 tcgataagcg tgattgcgcc aatccccgtg cgcgccagcg cttccgccgc ccaggaaccg
    42661 acaccgccaa taccgaccac acaaatatgc gcgtcagcaa acagttgcag cgctttttca
    42721 ccatacagac gcgctgtgcc accaaaacgc tgacgccatg catcactaat taccacagac
    42781 atagcacctc agaattaaaa agggcgaggt tacccctcac cctaaacgca taaatgcctg
    42841 atgcgctacg cttatcaggc ctacataact cctgcaatat tttggatttg catgatttgt
    42901 aggcctgata aggcgttcac gccgcatccg gcatgaacaa cgcgcactta gtcaataatc
    42961 aggatgtgag gttatccctc accctgtcat atccgtaaaa acggcataca gaatatcaca
    43021 tcagccgcta aagacgttac ctgcgcccgg agccgttttc agcacccaaa cccgtccata
    43081 gtggttgtac caacctgcgc ggtgtccggc ttccggcccg atcccttgat agatatcgaa
    43141 gtgttggcct ttgattgcgc caccgacatc cagcgccacc atcagacgca gttcatactg
    43201 accgttaaat ttgccgttat tatccagcag cggcacttct gccagcaagg ttgtacctgg
    43261 cggaataatg gaacgatcag aggcaacaga agcacggcca accagcggca ccgcacttgc
    43321 ccctttcacc ggtgcaaaag attgcggttt aaagaaaacg aaagacgggt tctgttccag
    43381 cagctcgcga acctcggctt cactgtgcgt ttcgccccag tgacgaatcg cctgcatcga
    43441 catatcttct tttttcactt cgccacggtc gatcagcacc ttaccaatgc tgcgataggc
    43501 atgaccgttt ttccctgcat agctgaaaaa gttaagcgga ctgccatcac caaagtcgat
    43561 atacccacta ccctgcacat ccataatgaa gttatccatc agggagttac tgtaagcgag
    43621 aatatattta tcactcaatg cccccgcgta gatctccgca cgagacggca gacgaccacg
    43681 ttttggcggc atacggtaaa taggatactg gaactcgccc tggcgggtat ggcgcgcctg
    43741 aattaccggc gtgtaatagc ctgtaaactg cacgttacca tagttgtcgg caccttccat
    43801 ctgccaggca tcaatgccga actggcgcat attgcgggta tcaccgcctg cgcgcagcca
    43861 ctcttgcacc gcgttataaa cattactctg gttgccatac agacgcggtg acgaattacg
    43921 gatatggtta atttgctcgg caaaatcacc ggcgttaatc ggcgcgccaa cggcatctgg
    43981 ctggttcacc agagagaaag gctgggtaaa tttcccgtct ttatattgct gtccgcgatc
    44041 ggttggtttg gaagagcagg cggcaagcat tgccacaacc gtgcccataa gaaggtactt
    44101 tacccaacgt cctttcattg ttctcttctt caggttaaaa aataaggcgc aacgaagata
    44161 acaaaccacc gcgtttaatg aaatgagcag cctctccctg acgcaaattt tgcacaaaaa
    44221 ataggcttta gtgatttgtt tttgttcaaa atcatgccaa atccgtgatc ggggtaaaaa
    44281 aaaggttgca tgaaaacgcg agcggagtat agtgcgcatc cacggacgcg gggtggagca
    44341 gcctggtagc tcgtcgggct cataacccga aggtcgtcgg ttcaaatccg gcccccgcaa
    44401 ccaattattg aacaccctaa cgggtgtttt tttgtttctg gtctcccata aaaaagcgcc
    44461 attcagcgcc tttttatcat ccccttctcg ccagcgtcgc cccatcggca aaatacgctt
    44521 taatccccgc aagaatagac tccgcaactt cctgctggaa agtcgccgtt ttcagtttac
    44581 gctcttcctc aacgttactg ataaacgccg tttcgaccag aatggaggga atatctggtg
    44641 cctttagtac ggcaaacccg gcctgttcaa cttgattttt atgcagcttg ttgattttac
    44701 cgagcttatt cagcactgct ttaccaaact tcaggctgtc ggcaatggtc agcgactgca
    44761 ccatatcgaa catggtgtgg tcgacatagc ggtcaccgct tttgctcacg ccaccaatca
    44821 agtccgaggc gttctgggtt tgtgccagat attttgccgc agtactggtt gcacctttgg
    44881 ttgagagcgc aaacacagag gaaccgctcg gctgacgact ggtaaaggcg tcggcatgga
    44941 tagagacaaa caggtcagca cgctgtttct gggcttttgc tacgcgcact tgcaacggaa
    45001 tgaagatgtc ttcattgcgc gtcatgtaca ccttcatatt gccctctttc tcgatcagag
    45061 agcgcaaacg gcgagctatt tgcaatacca catctttttc gcgcgttttg tatttcccca
    45121 ccgcaccgga gtcttcgccg ccgtggccag ggtcgagcat aatgacaatc ggacgatcgc
    45181 gcccggcctt gcctggctgt gggccactct gcgcaggcgg cacctgcttt tcgaggtcgc
    45241 ctttattgta atcttccagc agcgccagca atgggtcctg catgtcctga gcattggccg
    45301 gataaaggtc catcaccaga cgttctttaa acccggcgac cggcgcgagg gcaaacagct
    45361 gcggttttac gttttgcttt aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc
    45421 cgacgcgcgc cgacttgatg aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga
    45481 gtaccgagtt caggtttaca tcttcgatat ccaccaccac gcgttccggg ttactcaacg
    45541 cgaactgctt atatttcagc tgacgatttg attctaccgt cacgcgggtg tagctggacg
    45601 caggccagac gcgcaccgcc acgacctggc tgaccgcagc caggctgacc tgacttacgc
    45661 tcaatagcca catggcaccc gcgccttgca gtaaacgacg acggctgatt gcagtgttgg
    45721 atcctgacat gcctctcccg agcaaaagaa atctaagctg tgtaacaagt aaacgactaa
    45781 tttgaccgga aactttagcg aaagacgcat aatctgtcat ctaataaacg gtaaacattc
    45841 tttttatatt cacggcatta ctgataaaaa agtcgctctc gcataaaatt tacacttgca
    45901 ccctgcgaaa aaacagaata aaaatacaat aatttcgaat aatcatgcaa agaggtgtgc
    45961 cgtggtaaag gaacgtaaaa ccgagctggt cgagggattc cgccattcgg ttccctatat
    46021 caatacccac cggggaaaaa cgtttgtcat catgctcggc ggtgaagcca ttgagcatga
    46081 gaatttctcc agtatcgtta atgatatcgg gttgttgcac agcctcggca tccgtctggt
    46141 ggtggtctat ggcgcacgtc cgcagatcga cgcaaatctg gctgcgcatc accacgaacc
    46201 gctgtatcac aagaatatac gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc
    46261 tgcgggaaca ttgcaactgg atattactgc tcgcctgtcg atgagtctca ataacacgcc
    46321 gctgcagggc gcgcatatca acgtcgtcag tggcaatttt attattgccc agccgctggg
    46381 cgtcgatgac ggcgtggatt actgccatag cgggcgtatc cggcggattg atgaagacgc
    46441 gatccatcgt caactggaca gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt
    46501 cactggcgag agctttaatc tgacctcgga agagattgca actcaactgg ccatcaaact
    46561 gaaagctgaa aagatgattg gtttttgctc ttcccagggc gtcactaatg acgacggtga
    46621 tattgtctcc gaacttttcc ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa
    46681 aggcgattac aactccggta cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag
    46741 cggcgtgcgt cgctgtcatt taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt
    46801 gttctcacgc gacggtatcg gtacgcagat tgtgatggaa agcgccgagc agattcgtcg
    46861 cgcaacaatc aacgatatcg gcggtattct ggagttgatt cgcccgctgg agcagcaagg
    46921 tattctggta cgccgttctc gcgagcagct ggagatggaa atcgacaaat tcaccattat
    46981 tcagcgcgat aacacgacta ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat
    47041 tggggaaatg gcctgtgtgg cagttcaccc ggattaccgc agctcatcac ggggcgaggt
    47101 tctgctggaa cgcattgccg ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct
    47161 gaccacgcgc agtattcact ggttccagga acgtggattt accccagtgg atattgattt
    47221 actgcccgag agcaaaaagc agttgtacaa ctaccagcgt aaatccaaag tgttgatggc
    47281 ggatttgggg taagcgacga aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca
    47341 tccggcaatt acgtttattc ccgcgaacta aacaacgccg ccagaccact gcgccgctca
    47401 gtacgagtgg cgattgccgc acttaatata cgctcatcgg catacagcga cagacggcga
    47461 cgcgcgcggg tcaccgcggt gtataccagc tctcgcgtta ctaccggcgt gcgttggctc
    47521 ggtaaaatca acgccgcatg gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc
    47581 cacgtagttt cgtgctctgg caagcgactc ggttgcacag acttaatatt gccgtccggc
    47641 atcgcaaacc agacgcgcgt cccctgcccg cgatccagcg caataccgat atcgccatta
    47701 aacaacccaa gcgcgctgtc attacgggca atcatcactg gtcgaccttc gtaccaacga
    47761 gagtgcggat gacgatgaat tttgcgcttc tgttgcataa actgctcaat tcgctcattc
    47821 agtccagcca cgccaaacgg cccttcccgc agggcgcaca aaagctggta ctcattgaac
    47881 gcctgaatga ttaaatccgg ctcggcacgc gcttgcagca gatccagata acgtccgtaa
    47941 cccgcaagag cttcctcaag catcgcaata taatcttcgc cgctctgtaa aagtcgtttt
    48001 tcgatatcag taaaatcctg ctgaaaaacg gttttcactg ccgttttatc accacggttg
    48061 atcgccgcag ctaactgacc aatgccagaa tcgctgccga aacgatagct tttttgcagc
    48121 aggcagagac tgtcgcgcaa agatgccgct tctgtgccag ttcctgccgg aacgtgagtc
    48181 cccgtcaggc ggcttagctg cctggcacgc tcggcggtaa agcccgcgtt ggcataagcg
    48241 cagatatcgc ccagcacagc cccagcctca accgaggcca gttgatcacg atcgccgaga
    48301 aagatcactc gcgcatgatc tggcaaggcg tcgatcagtc tcgacatcat aggcagatcg
    48361 atcattgacg cttcatctac caccagcaca tcaagatgca gcgggttacc ggcatgatga
    48421 cgtaaacgct ggctacccgg ctgcgcgccc agcaatcggt gcaaagtgtt ggcatcttcc
    48481 ggaatgcgtt tcttttgttc atcggtcagc ggtaactgtc gcaaagcctt gccgagagat
    48541 tcggttaagc gcgcggcagc tttacccgtt ggtgcagcca gacggatacg gcagcgttcg
    48601 ccgtcggcca tttgaattaa cgctgccagc aacttcgcca cggtggtcgt tttaccggtg
    48661 cctgggccgc cggaaatcac cgagatccgc cgcgtcagcg ccactgccgc tgcaactttt
    48721 tgccagttaa tttcatcgct tactggaaaa agtttgtcca gggtttgcgc cagtagagct
    48781 tcatcaacct cgatggcatg attcacctca ttgaagaagc gcgctaccgt ccgctcgtta
    48841 caccacatgc gattcaaata aagacgatcg ccacagagga tcatcggcgt gggttcatct
    48901 cccctgctga ccgcttgaga agccaacaag cattcttccc aattttgtag ctcaccgatt
    48961 tcactgacac aggtcgccaa cagcggatgc gacgcctcgt tattttccag tcgtgaaagc
    49021 ggcaaacaga cgtgtccctc tccggcatca tgacttaaca gtgccgccgc gagggtgacg
    49081 gcaggatgtt catctcccgc cacggtcagg gcaaattgca catccagcgg gcgtagctgt
    49141 ttgtgctcca cagcttccag taattgcttt tgcaatttca ttacgcctcc tccagggtca
    49201 taccggcaaa catctcatcc atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa
    49261 tcccttgttg cggatgttct ttatcaacgc cacgcaggaa cagatagata acgccgccaa
    49321 agtgacgatc atagtcgtaa tcagcaatgc gatggcgcag ataacgatgc agcgccaggg
    49381 tataaagctg atattgcaga tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt
    49441 gggtgtaagc cgaactgtct tcacccagcc agttggattt atagtcgagc aggtaataac
    49501 gcccttcgtg gcggaatacc aggtcgataa agccttttaa catgccgcgt acctgcatga
    49561 actccagcgg cgggcagcca gcggatagcg ggtcaaactg gcgaattagt gcatcaagct
    49621 ggctggcgat gagcggttcg ctaatcggca ggtaaaactc catctccacc tgtttatcgc
    49681 ggtcggaaag ctgactcagg ctaacacccg tttcattgag aggtgcctgg aggacagccg
    49741 tgatccactc ggtcaacacc ggttcccact gcgattcaaa gccgccgagt tccagttttt
    49801 cctgtaccca gttcgggtca accggctggg taaaatcgag gtcttcaaac aaactgtgca
    49861 agaatgtccc cggtgacgca ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa
    49921 cgacgctgac cacgcctgcg gcatcaacat ccagccgtgg catcagatcc tgagcgatac
    49981 cgtgaccacg ctgctgcaag ccggagtagc tggtgacacg ccagttatcg ccgggcaatc
    50041 gttgtagcgt cctcgcattc agttctgcag ttaaagcatc attaacctgc cagggctggt
    50101 tatcaccagt ttgtgccgtt tgccaggcaa tatcatcatc gcataacgct tcaatacagg
    50161 tgcgaagccc tgccgcatct tgcggttccc ctttttgcag caaacgcccg agcgcacttt
    50221 ggtggacgtc ggtgtcacct tttttatcgc cacgacggcg caccagcggt gcaacgccga
    50281 gactgcaatg ccaaaccgaa cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca
    50341 gacgttcggc ctccgcgaga tcgacacttt ctggcgcagc gttaagatcc agaacagcct
    50401 caaacgagtg gcgatcgtga taaaacgcct gatcctggac gcggaaattg gtaataaatg
    50461 gcaaccagac aagcggatat tccagccctt ttgatttatg gatcgtgaca atctgcacca
    50521 gatgtttatc actttcgagg cgcatttgtt ggctggaggc attactgtct ggctcgagga
    50581 tatgttgcga taaccagcgt accagcgcat gttcactttc cagctgcgtt ccggcttctt
    50641 gaagcagttc gctgatatgc aagatatcgg taagacgccg ctcaccgcct gccgttgcca
    50701 gcaagttttc tgcaatgtta cgcgccgaca tcagcgcccg cagcatcggc ataacgccac
    50761 gtttgcgcca gatttgccga taaccatcga actcttcaac taccgcatcc cacgcatgtt
    50821 cgtcattgtt cagcgtttca atatccagcg cattcagccc catcattgac gttgccagcg
    50881 cactgcgcag ggtgttctca cgttcgggcg tcatcaccgc ctgcaacaac caaagcattt
    50941 cctgcgcttc cagagtttca aaaacactgt cgcggttcga aaggtaaacg gaagggattt
    51001 ccagcaacgt taaggcatcg cgcacctggg cggcctcctg gcggctgcgc accagcacac
    51061 tgatgtccga agcacgcacc ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc
    51121 gctgtccggc ttgtagccag tcgcggattt gcgcagcaca tacctgcgcc atggtacttt
    51181 gatagtcgcc aacgccgcag ctttcgcctt ccatcagcca cattttcatc gcaggctgcg
    51241 tttcaccttt aaatacaaaa cgtaacgcct gatttttccc ggctgatttc actggaataa
    51301 acggtatttc gcgaaatatg aacgcgtcat cagtctggct gaaaagcttg ttcacgctgt
    51361 tcaccattcc tggtgcggaa cgccagttgg tgtctaaagt gtagtgggcg tgaacttcgc
    51421 tacgcgcctt catataagtg aagatatccg caccccggaa tgcatatatg gcctgcttcg
    51481 ggtcgccaat tagcaacaat gcggtttccg gctgatggtg ccagatacgg cgaaaaattc
    51541 ggtactgctg ggggtcggta tcctgaaatt catcgatcat tgccaccggg aatcgcgtac
    51601 ggatcgccgc tgccaacacc tcgccgcttt cgctacgcag cgcggaatcg agccgactta
    51661 acatgtcatc aaaacccaat tcgccacggc ggcgtttctc acgcgctact gtttcgcgga
    51721 tctcagccaa tgcgcgggtg atcaccagat cgcggatcga caatggttct gcaagcagtt
    51781 gatcgatcgc ctcaaacagt ggatgtcgtg gggtttcccc cccggccttc gtgcgatctt
    51841 ctaagaaacg ctgggagaat ttttccagcg actccggcaa ctgataactg tttgtctctt
    51901 cttctgccca ggcgctgatc ttgtcgatcc atttagcctg attgctacgg ttaaacttgc
    51961 gtcgatcaat accagaagat tcgatcagcg catccagttc acccactgcg tcgcgccact
    52021 gctgttttac cgtatcaata cgcgccacaa tttgcgcgtg acgggaagcc agcgtttcat
    52081 catcgggcgg cggtgctttg ataaccggcg cttcgccttg cagataacga ttaatatcgc
    52141 gcagcaacgc ctgcggccct ttccaggttt caaagacgac ctgggctatt tcacgcggca
    52201 gcgggtagca gtggcgacgc cagaaatcgg cgcaggcctg gtagcgtagc agagactcat
    52261 cttcaatcag ctgctgctca aacagcatgc cggattcaaa ggcattcagg ttgagcatgc
    52321 gctggcaaaa gccgtgaata gtaaagactg ccgcttcatc catctgccgt tcggctaaca
    52381 acaaccactg cgcggcttgc gctttatcgt cgatctcttc cagcaggcgt tcgtacagtg
    52441 gattgtcggt ggtttcacgc agacaggcga tgcgcaactc gtggatattg ctacggatac
    52501 gaccgcgcaa ttctgccgtg gcagcctcgg taaaggtgac caccagcagt tcttcaacgg
    52561 tcagcgggcg gggaaaggcg gcggaaccgc ctagtccaag taacaggcgc aaatagagcg
    52621 ccgcaatcgt aaaggttttg cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta
    52681 agggcaagcg caaaggatct agtgtctcgg cgacatcact cattcttttc actcatcagg
    52741 ggcattgttt gctgcaacgc gctgacgttc tcccacactt tccagccttc aggatgcaca
    52801 tattcggctt tcccgttctg gctgccggaa atctgcgaca gaatagccat gccttgcggc
    52861 tcgaccaccg cctgatggaa gaaatcagca agtttttgcg gcgtcagcag ttttatctgg
    52921 gccacgattt tatcacgcga atcgaagcgc atattgccgc gatcgaaatc tttacttaac
    52981 ttcgatgctt cttcgccgag cgtttgcggt gcctgcagca tctgggtaat taccgcctgc
    53041 tggatttgcg caaactcatc tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa
    53101 aacgccttgt aacgctccca caagaatgaa ggctgtttat cattgctttg caaaaggaag
    53161 cccatgcccc actgacgccc cacgctcatt ggaaacgcaa acacggcata gcccaattgt
    53221 tcttcggtac gcaactgatt gtagaaccac ggctgtacga tctgccccaa cagagagcta
    53281 taggctgagc tggtgtattc atcgtagcca gtcggtacaa ataccgctgc cagtgcggag
    53341 tcggtgctgt taccggcttt ttcaaagatg acggattgtt ttttatcgac cactacatct
    53401 ttgtttcgac accactctga cccatcagcg cccaactgtt tttgcacatc gcgtgccagc
    53461 gttgttgcct gggcctcggt catgttgccg ataaccataa actctggtcg agcccctgat
    53521 tttaaggcgt cgcgataggc cagcacctct ttcaaagtaa tggagggcaa aattttgcgc
    53581 cgttcatctc gcgagaagta cggcacttgc gagagcatct gcgcgggcat aatcgcctgc
    53641 tcgaacgctt tgcccttttc tgcggaatcc atcatctggt tataccagga cttcgcttgc
    53701 tcaagctgat cttccgtagc ggtatagcta aagtacccct cgagcaatgc ctggaacagc
    53761 tgcggcaggc gctgggtgta accattggca ttaaccataa ggccgttgtt agcgttggtg
    53821 gaaaaactta tgccaccaac cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg
    53881 agataatcat tgagcgcaaa catcacctga ttgcgggcgc tgtccatggc tttcggatta
    53941 cgcaaaatca ggctgacatc agctttgggt tcgctggaaa aataacggct tggcgcatac
    54001 accacgcgca gatttgactc atcaacaatc agctctggat ggtcatattt cttgtctgac
    54061 ttaatcagcg agaaatcgtc aggaatatag gggttaagct ccggtaagga gagcgcaata
    54121 ttggcagatt tttgctgcca gtcagcgaaa gtttgttcgc tgattttatc gacctgatac
    54181 ggcgcatcga caaaataagc cgttttattg tgcggctctt tcgggctgat ataccagata
    54241 cgcgcattct gcggcgtcat catcgccaga cgttctttta ctgctttagc atcgtaccga
    54301 tcggcaatat tgactgcatc cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc
    54361 agccattcga cgtaatccat atcacgggtg attgacggat aacggaagtc tatatccagc
    54421 acattcgcca gttcatcgaa gtattgttta tcgatccctt tttcacgtaa cagattgagg
    54481 tagctaaaaa tggccgccac aacctgatcg cgattcgcca gacctttatc ggttaaagac
    54541 gcagagatcg ctaatacacc gctgttgccg ttgacgatag gatcggagtt ggcgctaatg
    54601 ccctcaacta atccctgctt ttgcagccag tcagaaagtg tacctaggct gcgattacca
    54661 atcagatagg taatcaattc atccgtttta ctgcggaact tcgctgagtt gttatcgatg
    54721 cgaaactcaa cgcgcagaac tttacgcggc agcgctggga cgtaatgaat aataatgccc
    54781 ttttgggcgt cggtgactac cggcacggta atttccggtt ttttgctctc tttgttcggc
    54841 acgcgaccaa aagtgtccgc cgccattttt gccaactccg gcagcggttt attactgtaa
    54901 ataaccgcct tcatcagatt ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc
    54961 tgcaccggat taccaggttt gtcgcttaaa gtttcgaggt taccaccaga aaactttgaa
    55021 ccggggtgtg ccgggttaat ggtttctgcg ctgacttgtg ccatgcgcat cccgtcacgc
    55081 gtacgcgcca tggttaattc ggcattcacc gcattacgct cacgttcggc gtatttcttg
    55141 tcgagcaagg gttctgcaat agcgtccgcc aggcggtcta ccgcaccggg caaggcgtcg
    55201 ttctcaactt ccagatagaa agccgtgcga tacggtgccg tgctggcatt gtggctgcca
    55261 ccgtgcattt tgagatattc ggccagactg tcagcctgcg ggtacttttt cgaccccatc
    55321 agactcatat gttcaaggta atgtgccagc ccctggtacg cctcgggatc ttccagcgac
    55381 ccaacgggca ccaccagcgc cgagagcgat ttaactgcct gcggatcaga aaccagcaag
    55441 accaccatac cgttatccag acgtatagcc tgatactggc ggttatcttt atcactttta
    55501 cggatggttt cctgaatcgg ctgccatccc gtttctgcct gacttaaggg tgcccaaagg
    55561 gcaactaaca acaataatgc tttgaaccag gtgctgcggg gcattcacgg acctcataat
    55621 caactttatt ttctggccag attcaacagg ttaacgcaaa tcatctgcca gaatttaatc
    55681 ttgtgctgca cgagtcagcc tatgtttata taaccatcag tccgtgactg gtgcgcatca
    55741 taaagtaagc ggatggattg cgcaattttt atacaactct catgactgat taaagcgaaa
    55801 cagcggtaac aggaaacgtt gcgactgttc aacgattgcc tccattgtct ctggtgttaa
    55861 ttgccgccag agcctttgat accagatatc atcaccttcg ccacgcacca tcatgttgcc
    55921 ttcgtaagcc tgaaggaatt tcgtacgggc tttttgcaac gtggaatcgt catccagcat
    55981 ggcatcgttt tgcgcgtcat aacaggtttt tagccacgcg ccgccacttt caggtaacac
    56041 cagcaatggc gcggacatcc cttcacgata cccctcaatc agttgtgaga ggtaatgcaa
    56101 agcctgttcg gctgcaagcg gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg
    56161 actttcacca ttaccaccgc tggcacagta gacaaggtgt tccagccaaa gttgcattcc
    56221 ctgcgccaca cttaataaag agggacgcca gcgcaacaag ccatccggct gcacctgcgg
    56281 caaccagcca gttatctgca caccgttgca agcgagatca atttccatac tctgccccgg
    56341 ctggcgacag gcaatgactc tgtcggcaag ctgctgcatc tcctggcact gtgtttccca
    56401 gaaaatttca ccaaaagcgc catacggtaa atcccctgcc gcccggaagc ggcggaacaa
    56461 gcgttcggca tcatcctgct caaccagtgc attcaataac tgctgattga tttgataacg
    56521 gctaagtcct tccagaataa atggctcggt gtcggggatt tcgctgtctt cagtacggaa
    56581 gttcacctgc aaacgcatct ggaaaaacgc ccgcaccgga tgtgcccaga atcgttgtag
    56641 cgtttccagc ggcacggttt ccggtaaggt aaacggcagc ggctgaacaa attcagaatg
    56701 tgctttacca gcctggctgg ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc
    56761 tggctggtag ttttgtggat caaacggcat ccgggtatgg aggcaagtaa gatgcgcttt
    56821 tacccttgcc tcgctttcat cacagttgag cgcttcatcg cccggtagat aatgactttg
    56881 cccgatgtag tcgatcagtt cctgcaccag taccgacggg aaacgttcac tgttatcctg
    56941 aatggagcgc ccgatatagc tgatataaag tttttgttgc gcggaaatta acgcttccag
    57001 gaacagatag cggtcatcat cgcgacggct acggtcgcca cgcttcggtt tctgggtcat
    57061 caggtcaaag cccaatggcg caagctggcg tggataaacg ccgtcgttca ttcccagcag
    57121 gcaaaccact ttgaacggaa ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc
    57181 ggcgagaaaa cgctggctga tacgttcttg atccagacgc tgtgccagtt catcacgcaa
    57241 tagtgacagc ggcaccgcgt cgccatactg cgcacctaaa ccttcggcga taatcgcctg
    57301 ccattgttgt tcgatcagcg tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc
    57361 gttgagcata tcgcgacaaa ccggcaacca ctcttccagc ggacgctcct gtgccagccc
    57421 gcgacgccag atgtttagct gcattagcag tgaagccaga tgccccacca gttctgcaat
    57481 taagccgctc gattcatcat aaggtagaac cgattgccac tcgccctgcg cgctctccat
    57541 cgcgtagccc aacaacatac gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg
    57601 gagttccagc tcgcgaacgt tgtcgtcatc tatgccccaa cgaatgccgg actcgttgac
    57661 ccactggcgt aaataacgca gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg
    57721 cacatccagc agcgccagca catcctctga cacaaagcga ctgtcaggca gtgataacag
    57781 gctgataaac gcttccagta ccggatgtga ctgccgcgca cgacggtcgg aaatggcgta
    57841 aggcaggtaa cgatccgcag gtgcgctacc aaacactgcc tgaataaacg gactgtagct
    57901 gtcgatatca gccaccatca cgatgatgtc gcgcggagta agtgtcgggt cttcctccag
    57961 catcgccagc aggcgatcgt gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg
    58021 gaaggtgata ctgctatcca gtggatcaag cgggcgtttg ttatcgctac gggaaaactc
    58081 ttcgatgttc acaccagcaa cggcgcggtt ttccagttcc agaatgtcag actgaatgtt
    58141 atgcagcagg ttatctggcg tgacatcgac aaaggcgtcc agctcctggc tgctctccag
    58201 gtcagaaagg agataaatgt agtcgcgccc aagcttgccc catgaggcca gcagcgggtt
    58261 gccgacatcc tgttcaccgt cgctgttaaa gagctgtccg gcattttcgc tatcgcgaaa
    58321 tagaggcaat tcgcgatctt caaaactgtg tcgacgctgg cgggtcagca gtttcgccag
    58381 ataagcagga tctttaatat cgccccagta ataacggcag gggttggtaa acaggagatg
    58441 gatttcaata tgtttaccca gcgcctggag cgcctggaga taaacaggcg gtaacgcgga
    58501 aataccgcat ataaagacgc gcgaaggtaa ccccggcggg caggtcgtcg cggactccag
    58561 cgtttcgata aagcgctgat agagattcgc gcggtgccag cgcggttgtc cgagttcatt
    58621 tgtatattcc accagcgcct tccacaacgg cgcttgccag gcctgtgcct ctcccagccc
    58681 ttcaaccaaa tgtcctgttt cccactgtgc cagccagtcc ggacgataga ccagatactg
    58741 gtcaaacagg tccgccgctt ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc
    58801 agtcagataa tgccgcaaca gggtaaagtc ttcgtgctcc agcaattgcg gcagcagagt
    58861 catcagtttc cagctcatgc tctgtttgtt aaaggcgctc tctttgggga tctccggtaa
    58921 cacccggacg aacatatccc agataaagct cgcaggcagc ggaaaatcaa tgtttgccgc
    58981 aataccaaac ttttgcgaca gggtcatttg cagccactgt gccataccgg tactttgcac
    59041 cagaatcatc tctggttcga aaggatcgtc cagccgttcg cgttcgacaa taaactccat
    59101 caacgcttcc agcacgtcca gacgattgga atggtagacc cttaacatag cggctcctga
    59161 ctactgacga ttcgggcaat gcaggcgggt catctcgcct tctctgcccc cgggtgaaac
    59221 tagcgtaacg ctgatgctga cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca
    59281 gttggcaggt ggcgaaatcg cgcgcagttg cgtttgctgc caaccatgcc gccagagctg
    59341 ttggtactgg tttctgctgg caagactgtt cattaatgtt cgctggaaac ccgataacgc
    59401 agtgacaatc atcaccatca gcaccatcgc caacattacc tccggcaggc taaagccttg
    59461 ttgattcttc agggaagctg acataacgcc ccctctttca gcggacaaaa atcgctccag
    59521 ccgcgtggcg aaaaaacaat gtttccatcg atgatttcac ctgttcgcca caacgaaacg
    59581 ccttcataac cggcaatcaa taaggcttca ttatctgcca ataaacgcaa acaaacccgg
    59641 gcatcggttc cagcgtactg cgagcactga actgctggct gcgtctgcca ggagtgcatt
    59701 tttccccacg ccagcgccga ctgaacgatg gcctggcggc gcaatgactg actttccatg
    59761 ctcacgcgag aggcaaaact gcgatcctgt tggctcattc cttgtaatag caagctaccc
    59821 aaaactagca gcatcaggac cagagccagt gacgaaacac ccttttcgcg gttcacaggt
    59881 tgaatcctgt cacgctatag ctggcattca ccacggtttg cggttcagac ttactggcag
    59941 cacgcatatt aaccgtcaac accggcgaga agccgctgac atcctgacgt acgacctgaa
    60001 aagtgtcgat aatgatggca tccggattag tgactttatc ccagccctta ccttcacagg
    60061 atgtcgcacc gcgtagcgtt tccagcacat gctccttcag acgaaatcca atctggtcgg
    60121 actcttttac cggttcgcga tcccagatac cgttactgtt cgcatcccac tgcacaatga
    60181 cacagtcacc ctgtccgaca atttccagcc cttcgccggt acagatgcca tgacaataac
    60241 ccgccctctg gagatgcttc gcgacggtaa atacccgcag ccagatttca tcttccagtg
    60301 ccagcttacg ggtgctcgtt aaactttcac gctgtaacgc aggcagaaag cgtgcagccc
    60361 ccaacaacaa tacgctactg atcgccatag caatcaacac ttccagcaga gaaaaacctt
    60421 gctcttttac aggcatcctt ctgtttctcc ttgctgacaa agccggagtc ttccccacgg
    60481 cgaaaccacc agccaccact cgcccgttga gtttttgaag cgaatatgcc cggcccatgc
    60541 ggtattgcgc aggccaaaga aagcaagcga aggtgtcagg tcgctcattt cgacttcggg
    60601 ccagcgtggc acaaagacca atggtgaact gccatgacag gtattggccc cagcagcgga
    60661 actcacaagg caccataacg tcccctccct gataacgctg atactgtggt cgcggttatg
    60721 ccagttggca tcttcacgta aatagagcaa atagtctcgc gcctggctgg cggtttgcca
    60781 tagccgttgc gactgctgcc agtattgcca gccatagagt ccacttgcac ttagcatgac
    60841 caaaatcagc atcgcgacca gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc
    60901 ggcagtatgg agcaaggaga aaaaaagacg agggccagtt tctatttctt cggcgcatct
    60961 tccggactat ttacgccgtt gcaggacgtt gcaaaatttc gggaaggtgt ctcgaagaat
    61021 ttaatggagg gtaaaaaaaa ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta
    61081 attagatagc caccggcgct ttaatgcccg gatgtggatc gtagccttca atatcaaaat
    61141 cttcgaaacg gtagtcgaag atggattcgg gtttacgttt gataatcaac ttcggcagcg
    61201 gacgcggttc gcggcttaat tgcagatgag tttgatccat atgattgctg tacaggtgcg
    61261 tgtcgccccc cgtccagaca aaatcgccca cttccagatc gcactgctgc gccatcatat
    61321 gcaccagtaa cgcgtagctg gcaatgttaa acggcaggcc gaggaagacg tcacaggagc
    61381 gctgataaag ctgacaagag agtttgccgt ctgccacata gaactggaag aatgcatggc
    61441 acggtgccag cgccatttta tccagttcgc ctacgttcca cgctgaaaca ataatgcggc
    61501 gcgaatccgg gtcgtttttc agctggttca gtaccgtagt gatctggtca atatgacgac
    61561 catccggcgt tggccaggcg cgccactgtt taccatacac tggcccgagg tcgccgtttt
    61621 catcggccca ttcgtcccag atggtgacat tgttttcgtg tagataagca atgttagtgt
    61681 cgccctgcaa aaaccacagc agttcatgga tgatggaacg caggtggcaa cgtttagttg
    61741 tcaccagcgg gaatccatcc tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg
    61801 ttccggttcc ggtacggtcg tttttctgtg tgccttcgtc gagcactttt tgcatcagtt
    61861 ctaaatactg tttcatggtt cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc
    61921 agaccatcat gatcacaccc gcgacaatca tcgggatgga aagaatttgc cccatgctga
    61981 tgtactgcac ccaggcaccg gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga
    62041 tgcgaaacgc gccgtagcca atcaggaaca agcctgagac agctcccatt ggccgcggtt
    62101 tacgaatata caggttgagg ataataaaca gcaccacacc ttccagcagc agttcgtaaa
    62161 gctgtgatgg gtggcgcggc agcacaccgt aagtgtcgaa aatggattgc cactgcgggt
    62221 tggtttgcag cagcaaaata tcttctgtac gggagcctgg gaacagcatg gcaaacggga
    62281 agttcgggtc aacgcggccc cacaattcac cgttaataaa gttgcccaaa cgcccggcac
    62341 caagaccaaa cggaatgagt ggtgcgataa aatcagaaac ctggaagaag gaacgtttag
    62401 tacggcgggc gaagataatc atcaccacga taacgccaat caggccgccg tggaaggaca
    62461 tgccgccgtc ccagacacgg aacagataca gcggatcggc cataaactgc gggaaattgt
    62521 agaacagaac ataaccaata cgtcccccga ggaagacgcc gaggaagccc gcatagagta
    62581 agttttcaac ttcatttttg gtccagccgc tgcccggacg attcgcccga cgtgttgcca
    62641 gccacattgc aaaaatgaaa cccaccagat acatcaggcc gtaccagtga agcgccacgg
    62701 gtcctattga gaaaatgacc ggatcaaact ccggaaaatg cagatagcta ctggtcatct
    62761 gtcaccacaa gttcttgtta tttcgctgaa agagaacagc gattgaaatg cgcgccgcag
    62821 gtttcaggcg ctccaaaggt gcgaataata gcacaagggg acctggctgg ttgccggata
    62881 ccgttaaaag atatgtatat gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc
    62941 cgcgacgctc cataaaggct gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca
    63001 gactacgctg cgcaagattt tctgcttcgg caaaatcaat gcgccgcagc aggtatttta
    63061 cgcgcgctac agaacgtccg ttcatagaca gatggcgata cccaagccca atgaggattg
    63121 ccacgcacat gggatcgccc gccatttcac cgcacaaacg gagatcgatt ccatgtattt
    63181 ccgcttcccg ggcgatcatc gccagagctc gtaacattgc aggatgaaga ctgtcataaa
    63241 tgttcgccac ccgggtattg ttacgatcaa cggccaggat gtattgagtc agatcgttgg
    63301 tgccaacaga gataaaatcg acccgctttg ccagatgcgg cagcataaat accattgacg
    63361 gtacttccag catgatgccg atacgtggtt tgggaatttc gtaaccgatc atctcctcga
    63421 cttcacgtcc ggcacgttca atcaggcggc gagcttcgtc aacttcatcg aggcttgtga
    63481 ccatcggcaa cagaatattc aggttgcccg tagcggcgtt agcacgcagc atcgcccgca
    63541 cctggatcaa gaagatctcc ggctgatcga gcgtaatgcg aatcccacgc caacccaggc
    63601 atggattctc ttcgctgatc ggcatgtaag gcagctgctt atctgctccg acatccagcg
    63661 tacgcaaggt gacgggttta tcattgaaca tttgcagcat cccctgatac tgcgccacct
    63721 gttcttcttc cgacggaaaa ccactttgca gcatgaatgg gatttcagtg cgataaagac
    63781 cgatgccatc aatacggctg cccagttttt cttcatgttc cgggcttaaa ccagcattga
    63841 gcatgacttt tatacgctca ccgcttttta actgcgcggg taaattgacg tcatcttccg
    63901 ccagacggct aagctcaatc tcttcactaa ttagccgctg atattcttgc agcagtaccg
    63961 gctccggatc gaccagcaat tcaccgcgat agccatcaac gatcagcgtc ctacgatgca
    64021 gcaccgaagg ctgaatatcc gcgcccatca cggtagggat ccccagcgca cgtaccatga
    64081 tcgcagcatg ggagttggct gcgccatctc gcacgacaac acccactaag cgatcctggg
    64141 gcagctcagc aagagttgtc gctgacagtt catctgccac cagaatgaaa cgttccggcc
    64201 aggcgttcgg cccttgatta gcgtcatcaa gatgaaacag caatcgctga cccagcgcac
    64261 gtaaatcgcc agcgcgctct ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa
    64321 atttttcaat gaccgttttt accgcccact ctgccaccga gcctttatca acctcggcaa
    64381 acaattcgcg acgcagccgg gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg
    64441 ccgttttttt ttgtgcaccg gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg
    64501 cttcttccag cgccccggtc agtcgttcgc gttccagagc cggatccagc gttgatgcct
    64561 gatacacctg ttccattaaa ggtaacgtgg catcctgcca gccttcggca atcgccacac
    64621 caggtgctgc cggtaatgcg cggattcgcg tctggcgata ttgcccaaac aaggcagtca
    64681 actgcgactg agaaagaata gctgccatct gggtggcaag cgtcaccagg aaggattctt
    64741 cactttcgtc atactggcgc aactctcgtt gctgtaccac cagtacacca agcaactggc
    64801 gacgttgaat aattggtacg cctaaaaacg cgcggaaacg ttcttctttt acggagggga
    64861 tgtatttgaa gctggggtgc ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc
    64921 caaccaggcc gacgatccct tcatcaaacg caagcgttac agtgcgaccg cgtggttttt
    64981 tcagcccccg ggtcgccatc aggtagtaac aacgtcgatc atgatcggcc aggtagaccg
    65041 aacagacctc ggtatccatc gcaagacaga tgtcggtaac cagaatattt aacgcctcat
    65101 tcaggcgtgg tgcgctggct accttttcga ctatttcgcg caggcgagtg agcataattg
    65161 gcgtgactta acctctttta cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt
    65221 cctgcagtga catcaccacg ctcgcgaact ctttcattac cctacggtag acatcacgtt
    65281 taaatgacac cacctgtctg accggatacc agtaacttac ccatcgccag ccgtcaaact
    65341 ctggtgtact gctggtttgc atattgattt ctgcatcgcc gctcaccagc tgcaagagaa
    65401 accatttttg tttttggccg atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg
    65461 gtaatttgta gcgcaaccag ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta
    65521 atcctacttc ttcaaacaat tcacggtaca tcgcctgctc tgcggattct ccggggttaa
    65581 ttccgccttg cggaaattgc caggagtgct gaccaaatcg ccgggcccac attacctgcc
    65641 cctggcgatt acaaatcacg atacctacgt ttgggcggta gccatcgtca tcaatcaccg
    65701 gactacctca atataaagct ttatatacga atgattgttt catactccag gaagacggta
    65761 aaccactctc tgcagggcat tacacactaa taacaattga ataactcaca gttatgtgca
    65821 gagttataaa cagaggaagg ggtggatagc cgtttttatt caccttttct gtggatagag
    65881 ttgtgaagaa ctacggaatt actacgggaa aacccggagc attctgaata agcccgatat
    65941 agacatttta aaatattcat tatcagtcaa tgcattacat gtaatttaag taatgaaaaa
    66001 gagtaattcg tgacccaggt cacacctctc atttacgggt tggcgaaaga tcaaccaatg
    66061 ccgtatttat ccacagaatg tgccactaag ttaagcactg aaccactaaa aactggagtt
    66121 tcgtcgcacg tcaaggctgt aaatggaaac agtagtggag gtttttcaca gttatcccag
    66181 ctttctgtgg ataacatggt gtaagatcct gtttattttc agtgaccaga tttggaaaac
    66241 ccgttgcagt gttgcgcaac tcgattaccg gcaacctaaa aagcaatata aatcagagaa
    66301 ttgaacaacg catgtggaaa aagttacact gcgaatattc ggcacataat tgctgtttgt
    66361 tttttaacca aggtatcatg acatgtccca acctcgccca ctgctctctc ctcccgaaac
    66421 tgaagaacag ttgttagcgc aagcacagca actttctggt tatacattgg gagaactggc
    66481 ggcacttgcc gggctggtta cgccagagaa tttaaaacgc gataagggct ggattggcgt
    66541 gttactggag atctggctag gtgccagcgc agggagtaaa cctgagcaag attttgctgc
    66601 tctgggcgtg gaacttaaaa ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac
    66661 attcgtttgt gttgccccgt taacgggcaa tagcggagtg acctgggaaa ccagccacgt
    66721 gcgccacaaa ctcaaacgcg tactgtggat accggttgaa ggcgagcgca gcatcccgct
    66781 ggcgcagcgt cgcgttggat caccgttgct gtggagcccg aatgaagagg aagaccggca
    66841 gctacgcgaa gactgggaag aattaatgga tatgattgtt ctcggtcagg ttgagcggat
    66901 taccgctcgt cacggggagt atttacagat acgaccgaaa gcagcgaatg cgaaagcgct
    66961 taccgaagcc attggtgccc ggggcgaacg gattctgacg ctgccgcgcg gcttttattt
    67021 gaagaaaaat ttcaccagtg cgctgctggc ccgtcatttt ctgatccagt agccatcgct
    67081 ttgacctgcc gctttccggg catataatta ccgcttcatt tttttggcag ggctttttag
    67141 atgttatttg catggataac cgatcctaac gcctggcttg cgctcggtac gctgacgctg
    67201 ctagagatcg ttcttgggat cgacaatatt attttccttt ctctggtggt ggcaaagctt
    67261 cccacagcac aacgtgctca tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt
    67321 ctggcgctgc tggcatcaat cgcctgggtt acgcgcctga caaatccgct ttttacaata
    67381 ttcagtcagg aaatttctgc ccgtgacttg attctgcttc tgggtggctt gttccttatc
    67441 tggaaagcca gcaaggaaat ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca
    67501 cgcgtttcat cattcctcgg cgctatcgtg cagattatgc tgctggatat tatcttcagc
    67561 ctcgattcag tgattaccgc tgtgggtctg tcagatcacc tgtttattat gatggcagcc
    67621 gtggtgattg ccgtaggcgt gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa
    67681 cgccatcctt cggtaaaaat gctggctctc tctttcctga ttctggtggg ctttaccctg
    67741 attctggaaa gtttcgacat ccacgtaccg aaaggttaca tctacttcgc gatgttcttc
    67801 tctattgcgg ttgaaagcct caacctgatt cgcaacaaaa agaatccgct ctgatactcc
    67861 gtacgctctc ctgcgggagg gcgtactcac tcctgttacc ctcacagttt aagattttcc
    67921 tgctttcaag atatataacg tcggtttata aacagactat tatcataggt gggccaaatg
    67981 aggataaccg gatgaaaaaa tgggcagtaa taatttctgc agtcggactg gcgtttgctg
    68041 tttccgggtg ttccagtgat tacgtcatgg cgaccaaaga tggccgtatg attttgaccg
    68101 atggaaaacc tgaaattgat gatgataccg ggctggtgag ttatcacgat cagcaaggta
    68161 acgcgatgca aattaaccgt gatgatgttt cgcaaattat tgaacgttaa caaataaggt
    68221 cagcatccgg ctggccttaa gatttttctc tttccctttt cccttccctc tgccattttt
    68281 atattcctta tgtcgtgatt ataaaaagga aacggctatg caatatcacc gtatacccca
    68341 cagttcgctg gaagtcagca cgctggggct tggcacgatg acgtttggtg aacagaacag
    68401 cgaagccgac gcccacgcac aactcgacta tgccgtcgct cagggcatta accttatcga
    68461 cgttgccgaa atgtacccag tacctccgcg ccccgaaacg caagggttaa ccgaaaccta
    68521 cgtcggcaac tggctggcga aacatggcag ccgcgaaaag ttaattatcg cctccaaagt
    68581 gagcggaccg tcgcgcaata atgacaaggg catccgcccg gatcaggcgc tggatcggaa
    68641 gaatatccgc gaagcgctgc atgacagcct caagcgccta cagactgatt acctcgatct
    68701 ttatcaggtg cactggccac agcgcccaac caactgcttc ggcaaactcg gttatagctg
    68761 gacagattct gcgcctgcgg tttcgctgct ggatacgctg gacgcactgg cagagtacca
    68821 acgcgcggga aaaattcgtt atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg
    68881 ctacctgcat ctggcggaca aacacgatct gccgcgtatt gtcaccattc agaaccctta
    68941 cagtctgtta aaccgcagtt ttgaagtagg tctggcagaa gtcagccagt atgaaggggt
    69001 cgaactgctg gcctattcgt gcctgggttt cggcacgctg accgggaaat atctcaacgg
    69061 tgcaaaaccc gctggcgcac gtaatacgct ctttagtcgg ttcacccgct atagcggtga
    69121 gcaaacgcaa aaagccgtcg cggcgtatgt tgatatcgcc agacgtcatg gcctggatcc
    69181 tgctcagatg gcgctcgctt ttgtacgccg tcaaccgttt gttgccagca ctctgctggg
    69241 cgcaaccacg atggagcagc tgaaaactaa cgtcgaaagt ttacatctgg agttaagcga
    69301 agacgtatta gctgaaattg aagcggtaca tcaggtttat acttatccgg caccataaag
    69361 attaacgccg gttaaaaccg gcgttaaata ttaatgacgg cgctgccaga tccacagcgc
    69421 cgttattgcc agcgcaaaca gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc
    69481 taccattacc gccagcgagt aaatgcccag catcaacaac atggcgctgt tttcgccaag
    69541 gttttgtact gcaatcgcat tccccgcccc gacgcttttt ttaccccgct cctgtagcaa
    69601 cgcattgagc ggaacgacaa aaaagccccc cagcacgcca atcagcatca acaaggcata
    69661 ggctggcaac agctcgtgtt gcagggagaa aatcagtacc accacgccaa tcaaaatccc
    69721 ggctggcata cagcgtgaca cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc
    69781 aaccacgata ccaatcgcta ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat
    69841 gcccagcgcc accggtaccc acagcaccaa caggaaacgc agcgttacac ccgctcccca
    69901 gaataaactg gtgcccacca gcgaaaaacg cgtttcacca ttgcgccata gcgaggtgca
    69961 ggcattcaga aaactgcggg tcatgttgat gagattccag gactgccccg gacgcgccgc
    70021 cgccagtttg ggaatgtaga tattggcaac gaccgcacca ccgtaggcca gtgcgcatgc
    70081 ggccagggca accaggacat gccagtcagc cagcacacca ccggctacgg aaccgagcaa
    70141 aatcgccgct atggtagaag cttccattaa accgttagct tttactaact tactacccgt
    70201 ggttaattcg ccgagaatac cgtatttcgc cggtgaatag gctgcagcac caacacccac
    70261 cagcgtatag ccgagaaacg gattgatacc aaagcagata ctggctgcgc ccagcagctt
    70321 cagaccgttg gcaaacatca tcacccggcc tttggcgaag ctatccgcca cctgcccgac
    70381 aaacggcgca aaaagaatgt aagcacctac aaacaccatt tgcaggatgg gctggctcca
    70441 ctccggatag aactgcgctt tcagtaacgc cagagtggca aacagtagcg cgttatcgcc
    70501 aaacgcagag agaaactgcg ccacgataac cgctttcatc cccttcgacc acaacgaagt
    70561 gttagtgtgc actgactcac tcatcgtgtt gttccgcttc gtctacccag cttttcaacg
    70621 tgacaaagtc aggtttgccg ctgccaagta atggcatctg tttcagatag cgaatatcac
    70681 gcggtacagc aagctccggc acgccgtgct cgcgggcata ctgttgcaac ttatcgcgcg
    70741 tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc tttgctggca tcgctcttaa
    70801 tcgcagtggc atggacttta tctggcgaaa caccaagtgc cagttgttcc accatttcca
    70861 acgacaccat ttcgcctgca attttggcaa agcgttttgc gcggccctga atctgcacaa
    70921 agccctgctc gtcaaaacgc acgatatcgc cagtgtcata ccagccgcgc tccatttcgc
    70981 cgcgaacatt ctcggcggtg ggcacttcca gtacaccagg cttctccacc cgcagatagc
    71041 cgttcattat gttcggccct ttcagttgca ggcgtccgcc ctcttcgata ccagggaccg
    71101 acaacaggcg cgcatccata ccgggtagaa tacgccctac cgtaccgggt ttcgccgcca
    71161 tcggtacgtt gatagaaacg acaggcgcgc attcggtcac accgtagcct tcaaggatgc
    71221 gcaggccaaa tttatcctgc caaagctgtt tggtactttc ttgtagtttt tctgcgcctg
    71281 ccaccacata gcgtagacga tagaagtcat acgggttggc aaagcgcgcg tagtgaccga
    71341 ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc atacaccaac tccggcacaa
    71401 tgcggtaatg cagcgggctt ggataaagga acacttctgc acctgtgagc agtggcgtaa
    71461 acaggcctac cgtcagccca aaggagtgaa acagcggcaa cgccgacata aagcgatcgt
    71521 tggtggtgaa gtcggcaatc gttttaatct gctcgacatt cgccagaata cttttatggc
    71581 tatggacaac gcctttcgga tggccttcag aaccggaggt aaagaggatc agcgcctctt
    71641 cttccggctg ctgttttacc tgtgccagac gcggcatcag caaatgagcg aagatccata
    71701 ctttgtcggc agtggtgaca tctgctttta aatcttccag atagacccag cgcacctgag
    71761 taagttgctc cggcagatgc cagagtttgc ctttatcgag aaactggcgg gaagtgaaga
    71821 tggttttgat ttcagccgcc gtaatggcgc tggtcagccc ttttacccca gcggtgtagt
    71881 tcatcattgc gggaatgcgg cgacgggcga tggccccaaa aatcactgcc gcactgatgc
    71941 ctgcattggg cagcattaag ccgatgcgtt cgccttcaac actgtatttt tcaaggatgc
    72001 gtccaacaaa cagcgtttta gtaagcaatt tgcgatagga gtctggggta aagttgacgt
    72061 cttcgacaca tttcttcccg gctccgaagc ggtacattgc actcagtaaa gattcgtaca
    72121 gcgtttcacg cgggcgcacc gccatacgcg cttccatcat tatttgatgc agcatttcgc
    72181 cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg catctccacc tgcgttggtg
    72241 gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt aaccagacct ttcaggcggc
    72301 tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat aaccgttgca ccagacttcg
    72361 ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga gcctgtggtg gtgatgcgtc
    72421 cttcagggaa aatcaccact ggtcggccct gttccaccag acgtaccaga tgtttaatcg
    72481 ccataggttg cgtcgggtcg agaggaacaa agtcgataaa tgatttcagc caacgcatat
    72541 accactgttg acttattgag gtgtaaacgg caaataccgg acgtacgggt aaaaacaatg
    72601 ccagcagtat gccatcaata aaagagacgt gattaggcgt gattagaacg cgctcgccct
    72661 tcaatgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa aacacggcac aaatttcgaa
    72721 aaaagctaaa aagcattcca actccctttg ctctgattca gtaaaagcga atggagggag
    72781 attacacgag ataaagaacg cgagcgacag taaattaggt gcgaaaaaaa acctgcgcat
    72841 ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta ccgaataatc tcaccaatca
    72901 atacctctgg gatcttgatt gtggtctgca cgacgactct tcgccagcga gaaaacgcaa
    72961 aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg ttactgttac agattgatga
    73021 ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta attcatgtgc tcaacccgaa
    73081 gttgacttca cctatcaata cctctgggat caccacttta gcaacctgaa gccaaacgcc
    73141 accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc aaaggtttgt aatctctttt
    73201 tttgtcttac ttatttgcgt ttggctcaca ttcccacgat gaaaacacgc caccccttga
    73261 accaacgggc gttttccgta acactgaaag aatgtaagcg tttacccact aaggtatttt
    73321 catggcgacc ataaaggatg tagcccgact ggcaggcgtt tcagtcgcca ccgtttcccg
    73381 cgtcattaat aattcaccca aagccagcga agcgtcccgg ctggctgtgc atagtgcaat
    73441 ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg ctggcgcagc agaccactga
    73501 aacagtcggt ctggtcgttg gtgatgtttc cgatccgttt ttcggtgcaa tggtgaaagc
    73561 ggtcgaacag gtggcttatc acaccggtaa tttcttattg attggcaacg gttaccacaa
    73621 cgaacaaaaa gagcgtcagg ctattgagca actgatccgc catcgctgtg ctgcgttggt
    73681 cgtccatgcc aaaatgatcc cggatgccga tttagcctca ttaatgaaac aaatgcccgg
    73741 tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac cgttgtattg ctctggacga
    73801 tcgttacggt gcctggctgg caacgcgtca tttaattcag caaggtcata cccgcattgg
    73861 ttatctgtgc tctaaccact ctatttctga cgccgaagat cgtctgcaag ggtattacga
    73921 tgcccttgct gaaagtggta ttgcggccaa tgaccggctc gtgacatttg gcgaaccaga
    73981 cgaaagcggc ggcgaacagg caatgaccga gcttttggga cgaggaagaa atttcactgc
    74041 ggtagcctgt tataacgatt caatggcggc gggtgcgatg ggcgtgctca atgataatgg
    74101 tattgatgta ccaggtgaga tttcgttaat tggctttgat gatgtgctgg tgtcacgcta
    74161 tgtgcgaccg cgcctgacca ccgtgcgtta tccaatcgtg acgatggcga cccaggctgc
    74221 cgaactggct ttggcgctgg cggataatca ccctctcccg gaaatcacta atgtatttag
    74281 tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg ctggaggcaa gtcatcatgc
    74341 aaccagcgac taaccgcagt taaagcaatt ccagcgccag taattcttcg atggtctggc
    74401 gacggcgaat caaccgcgcc tgaccattat caaacagaac ttctggtaac agcggacggc
    74461 tgttgtagtt ggatgacatt gatgcgccat atgcccccgt atcatgcagt accagataat
    74521 cccccgcctt cacttccggc aaggcgcggg tttcaacatt tcccccttcc tgctgggtaa
    74581 agacatcgcc cgattcacat aatggcccgg cgacgacggt ttccaccgtt ggcgcgtgtt
    74641 ccagagaacg accatcagct gccagggcac tgatatggtg gtaactaccg tacattgccg
    74701 ggcgcatcag atcgttgaac ccggcatcaa ccagcacaaa gtggcggcta cccatttgtt
    74761 tgacgctccg cacctgagta attaacacgc cagactgcgc taccagaaag cgacccggtt
    74821 caatttccag tttcacaggg tggcccaaat ggcgggcgat ttgctgacgc gcggcattcc
    74881 acagaccata ataatgctcg gtatcaaccg cctcttcacc ctgttgataa ggaatagaaa
    74941 gcccaccgcc tgcagaaata gcctgtaaat cctgaccgaa ttcgatgacc tgacgtacca
    75001 tagcaccaca aacttgttcc agatgggcat aatcaacgcc agaaccaatg tgcatgtgaa
    75061 tgccgaccag ctgcagatga tgacgttgta tcacgtccag tgcggcgggc agatcggtgt
    75121 accagatacc gtgcttgctg ttttcgccac cggtattggt tttttggcta tgtccgtgac
    75181 caaaccccgg attaacgcgc agccataccc gatgccctgg cgaaacctgg cccagttggt
    75241 cgagcatatc aacagaaccc gcattcaccg gaatttgcaa tgcactgacg cgttcaagcg
    75301 tcgcctgatc gataacatct gccgtaaaaa caatatcatc ggggtgcgtt tgcggattgt
    75361 aacccgccgc caacgcgcgc tctatttcgc ctaacgagac ggaatccact ttcacgccct
    75421 gctcacgcat taagcgcaaa atatgaatat tggaacaggc tttctgtgca aagcgcacca
    75481 catcaaactg tttcagcgct gcaatctgcc gacgaataat ttgcgcatcg tagacccaca
    75541 ccgggcagcc aaattcagcg ggcaaacgca gcagattttc ggcggtgaga tcggtatcgg
    75601 tgctgaacag tgaatgtggc ataacaaact cctgatgagt gcttttttat gattacgcca
    75661 catcataaaa agaataaaaa atatcgattt atgtcgagtc tatgcaaaaa tgatatggat
    75721 taccggattg cgagagagcg ctaatggccg ccgttaactt acgtcatatt gaaatttttc
    75781 atgcggtaat gaccgccgga agcctgactg aggcagcaca cctgctacac acctcacagc
    75841 caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat cgggctaaaa ttgtttgagc
    75901 gcgtacgtgg acgattacat cctaccgtgc aaggactgcg tctgtttgaa gaagtgcaac
    75961 gatcctggta cggactggat cgcattgtca gcgccgcaga aagtctgcgc gagtttcacc
    76021 agggagaact gtctattgcc tgcctgccgg tcttttcgca atctttttta ccgcagctcc
    76081 tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa tatcgtgccc caggaatcac
    76141 cgctacttga agagtggctc tcggcccagc gtcatgattt aggactcact gagacactcc
    76201 atacgcctgc gggaacagaa cgtaccgaat tactctcttt agatgaagtg tgtgtgttac
    76261 ctccgggtca tccgctggcg ataaaaaagg tattaacgcc ggatgatttt cagggtgaga
    76321 actacatcag cctttcccgt actgacagct atcgccagtt gctggatcag ctatttactg
    76381 aacatcaggt taaacgacgc atgatcgtag aaacccacag cgccgcgtca gtgtgcgcaa
    76441 tggtacgggc gggggtaggt gtttcggtgg ttaacccgct caccgcactg gattatgcgg
    76501 caagcggttt agtggtgcgg cggttcagta ttgcggttcc gttcaccgtc agcctgatcc
    76561 gccccctgca ccgcccgtca tcagcgctgg tgcaggcgtt tagtgagcat ttacaagcgg
    76621 gattaccgaa actggtcact tctcttgacg ctattctgtc gtcagctacg acagcataaa
    76681 agcgacagca tcctcggcat ggatcgccgc agtatcaaac acaggcagaa cactgcgctc
    76741 ttctggcacc agtaaaccaa tctctgtgca gccaaaaatg acaccctgcg ccccctgttc
    76801 tgccaggcga gcaatcactt gcgcataata agtgcgtgac gcttcggtaa attgccccag
    76861 acacagttct tcaaaaataa tctgattaat tttcgcccgt tcatccgctt caggaataag
    76921 acaattgatg gaaaattgtt ccgtcagccg cccgcgataa aaatcctgtt ccatggtgta
    76981 acgcgtaccc agcagcgcca cacgagtcat tccggccccg gtaattgcac gtccggtggc
    77041 atccgcaatg tgtaagaaag gcagagagca acgagactct atagcatccg ccactttgtg
    77101 catcgtattg gtacataaca caatgccttc tgcgcccgcc cgctgtaagc caagcgccgc
    77161 ctcagccaga atatccccgg ttttgtccca ctcaccgcga cgctggcact cttctatttc
    77221 ttgaaaatcg acgctatgta gcagcacttg tgcagagtga agcccaccaa gccgctgttt
    77281 aatgccttca tttatcagac gatagtaagg aatggtggat tcccagctca ttcctcccag
    77341 caaaccaatt gttttcattc ctctctcctg atgtgtgtta ccgcaacaag gctgaataag
    77401 gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa aaagggccgg
    77461 atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt ctcgcctgcc
    77521 atcagtttgc gttcgatatg ttccagggtg acatttttgg tttccggaat gagccagaaa
    77581 gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc ggcagcgcca
    77641 atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac ccagttcgtg
    77701 gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc agagcacagg
    77761 atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat cgtcatgcca
    77821 acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat caggcaatag
    77881 cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc cggtttacga
    77941 cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga acataaaggt cagtcccacg
    78001 accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt gaagatacgc
    78061 ggcgcgtagt acatgatgat gttcataccg gtgaactgct gcatcgcctg caacaacata
    78121 ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca accgccctgt
    78181 tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc cgacgtatcg
    78241 cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc cagccagcgc
    78301 gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa agcaagaacc
    78361 cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa aaacgccagc
    78421 acgatgccga gtgtgaccat caactggtac atactgatca tcttacctcg aacgttttca
    78481 cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac agcaatgccc
    78541 agcaccacac gagcggcgat taacatctct acgctggtcg caaaagcgga ccctatagaa
    78601 ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag gcggaacgac
    78661 agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact aaccacccat
    78721 tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc tccggcgatt
    78781 acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga aacaaacata
    78841 ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt attgatagta
    78901 accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga acaactcaca
    78961 caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat gtcgttatgt
    79021 gatggatatt ccaattttca aattaagttg aattattgag attattatta accacctaat
    79081 tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga ttatttcata
    79141 accatgatat ggattatgat gatctacagg tataaaaaac cctgccatgc ggcagggtca
    79201 taaaagtaag aagaatgaat taacgcgcca gccaaccgcc atccacggca atggtataac
    79261 cattcacata atctgaagcg ctggaggcaa ggaacactac cggccccatc aggtcactcg
    79321 gcagtcccca acggccagct ggaatgcggt cgagaatttc cgcgctacgt tgttcatctg
    79381 cccgcaattg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca ttaacattaa
    79441 tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcaca ccgctttttg
    79501 atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac gcgatattga
    79561 taatcttgcc gccattgcct tgcgcgataa agtgtttcgc cgctgcctga gacatgaaga
    79621 atacgctctt gatattcagg ttcatgacat cgtcccagtc cttttcgctg aactcgagag
    79681 catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga ccaaactcag
    79741 ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg gcggtcaggc
    79801 ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt tcaacgatgt
    79861 taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc tggcccagcc
    79921 cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca cttaaaatca
    79981 taacaatacc ttattcgttt atgcccacaa ctagcgcaaa tctataacgg ccacatggtc
    80041 catatcatca aagacctggt tttcaccgac catgccccag ataaaggtat aagctttggt
    80101 tccgacgccg gaatgaatcg accagctcgg ggagatcacc gcctgctcgt tatgcatcac
    80161 aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt catcatccat
    80221 attgaaatag aaataaactt ccatccggcg ctcgtgagtg tgacacggca tggtgttcca
    80281 caagttacct ggagccagct ccgtcagccc catactcaat tggcaggttt ccagcacatc
    80341 cgggacaaaa tatttgttaa ttgtgcgacg gttactggtg aggttatcgc ctaacgtgac
    80401 tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg cgggtgcgca
    80461 attgtaataa aactttgccg gagtggcggt atcaatactg gcaaagacaa cttcttttgc
    80521 acctttacca acatacaggg cgtcgcggtg accgatttca tagcattgac catcgacagt
    80581 aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa ggaaatagct
    80641 tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta ccggcataat
    80701 gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat cggcgacaaa
    80761 taccttttca accaaaaatt cattgcgcag cccctgggta tccagcgttt ttgcgtgcgc
    80821 actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa gcgtgaaaat
    80881 agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat gcccatggga
    80941 tagggttttc aattaaaaat aaaacaatgt ttcattttta agttaaggat taaaaaaagt
    81001 gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca gaaaatgtat
    81061 gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt atcggtaagg
    81121 aggaaatgca gagctactcg tcacgttcaa tggtcaatgc cacgccctgg cccccgccga
    81181 tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga accagagaaa
    81241 ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg ccaccattga
    81301 cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc tgtgcagcaa
    81361 acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca cgctccaggc
    81421 aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca ccaacgctgg
    81481 caaatgcgcg gatgcgggcc agcacaggta aattcaacgc tcgtgctttg gcttcgctca
    81541 tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg gtcacagaac
    81601 cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg tcagtgcgtg
    81661 gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt accgggacga
    81721 tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc gagctaagtg
    81781 cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt tcggcggtga
    81841 caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga ctgtcaacca
    81901 actggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt ggtgcgcggc
    81961 tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt atcgcctgag
    82021 tagccagatg cagtgcctta agcccggaac cgcaaacgtc attaatcgtg attgcagaaa
    82081 cgctattagg aagaccacct ttaatagccg attgccttgc cggattctgc cctgcccctg
    82141 cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg ccggtacgtt
    82201 ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc gctaacgcac
    82261 cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct ttcatcacaa
    82321 cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat cttaattgtt
    82381 taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagcagtcag agacataact
    82441 ggcacgtaag gtttgcaacc actaacccac caatagaggg gtagataggg cgttaatctc
    82501 ccatacttaa cctggtttat ggtaaattgc cctctatttt gtttaatttg tagatgatac
    82561 gttcagataa tgtctaatat ttggtcaaaa gaagaaactc tgtggagttt cgcgctctac
    82621 ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc ggcaggggca
    82681 gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc acataaagcc
    82741 ttatgccagt tcatcctctc atcgaaaaca tcagcaggtg aagggataac gggcgcagta
    82801 acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt catcgccttt
    82861 tttgtcgtcg tgttgatata cgcagtggca attaccaact cacttacaga acagctggca
    82921 aagcatatgg tgattgatct tcgcatccgt atgttggtga gtctgggtgt tgtattaatc
    82981 ctgaatctca tttttctgat gggacgccat gcaaccatac gggtaatggg ttttttggta
    83041 ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag ttggcaacct
    83101 gatctattaa caacccaggt agaattcaat cagaataccc ttcaccagat atggatatcg
    83161 attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt tgccatagac
    83221 agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat gaaagtcgct
    83281 tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct ttctattcca
    83341 ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc gctttctatg
    83401 ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt agttgccatg
    83461 tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacaga ggtcgtcaaa
    83521 acaacactac agcaggttgg tataaagaaa agtcgtgcat ttaaccgcgc actatcaatt
    83581 atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc gatttcgatg
    83641 atttatgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc tacgctatca
    83701 acatatctca tcccggcgct taaaccctgg cgttccatcg gaaatctgat tacgctgatc
    83761 gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccattgtg aatggttcaa
    83821 aattgctaca attttgaacc attccatttt tcataatgtt catttgttat tattccgccc
    83881 tatcacttct ctcgtcatat ccttgaatta tcagttagtt aaacacacaa agaga
//
LOCUS       DAPGRN010000006        77994 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPGRN010000006 DAPGRN010000000
VERSION     DAPGRN010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.006"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            488..1003
                     /locus_tag="RHW33_000447"
     CDS             488..1003
                     /locus_tag="RHW33_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1643995.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRNNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1221..1949
                     /gene="artP"
                     /locus_tag="RHW33_000448"
     CDS             1221..1949
                     /gene="artP"
                     /locus_tag="RHW33_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV1643996.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1967..2698
                     /gene="artJ"
                     /locus_tag="RHW33_000449"
     CDS             1967..2698
                     /gene="artJ"
                     /locus_tag="RHW33_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1643997.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2705..3421
                     /gene="artQ"
                     /locus_tag="RHW33_000450"
     CDS             2705..3421
                     /gene="artQ"
                     /locus_tag="RHW33_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV1643998.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALALFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPMGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3421..4089
                     /gene="artM"
                     /locus_tag="RHW33_000451"
     CDS             3421..4089
                     /gene="artM"
                     /locus_tag="RHW33_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV1643999.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4418..5149
                     /gene="artJ"
                     /locus_tag="RHW33_000452"
     CDS             4418..5149
                     /gene="artJ"
                     /locus_tag="RHW33_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV1644000.1"
                     /translation="MKKLVLAALLASCAFGASAAEKINFGVSATYPPFESMDANNQIV
                     GFDIDLAKALCKQMQTECTFTNHAFDSLIPSLKFRKYDAVISGMDITPDRSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKISDQWFPQ"
     gene            complement(5200..6681)
                     /locus_tag="RHW33_000453"
     CDS             complement(5200..6681)
                     /locus_tag="RHW33_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV1644001.1"
                     /translation="MRAIILLFDSLNKRYLPPYGDALTKAPNFQRLAAHAATFENSYV
                     GSMPCMPARRELHTGRCNFLHREWGPLEPFDDSMPELLKKAGIYTHLISDHLHYWEDG
                     GGNYHNRYSSWEIVRGQEGDHWHASVAQPPIPEVLRVPQKQTGGGVSGLWRHDWANRE
                     YIQQEADFPQTKVFDAGCTFIHKNHAEDNWLLQVETFDPHEPFHTTEEYLSLYEDNWD
                     GPHYDWPRGRVQESDEAVEHIRCRYRSLVSMCDRNLGRILDLMDEHDLWRDTMLIVGT
                     DHGFLLGEHGWWAKNQMPYYNEVANNPLFIWDPRSGVKGERRQALVQMIDWAPTLYDF
                     FQQPVPPDVQGQPLAKTVSHDEPVRSSAMFGVFSGHANVTDGRYVYMRAALPGREDDI
                     ANYTLMSCKMNSRYPVDEMRALSLAPPFRFTKGLQVLRIPAQEKYKGLNQFGHLLFDL
                     QNDPQQLHPIHDDVIESRMIALLIQLMKDNDAPPEQFQRLGLA"
     gene            complement(6693..7013)
                     /locus_tag="RHW33_000454"
     CDS             complement(6693..7013)
                     /locus_tag="RHW33_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV1644002.1"
                     /translation="MKDTINILFVCGYGVGSSVMLQTVVKKALTKYDFKVEMEHTAAG
                     EVGGFTDWADIFAISKKLLDVVSLDPKQGQYLIPIENIMDGETIGQQIYEVVQQHYPH
                     LINQ"
     gene            complement(7035..8378)
                     /locus_tag="RHW33_000455"
     CDS             complement(7035..8378)
                     /locus_tag="RHW33_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV1644003.1"
                     /translation="MEGVPTMFAKFIDVIQTFLTEPAILIGLLVGIGYALDKKSSIKI
                     ITGMVSAMVGLMMVLFGGFQFSATFKPVAEAVSKAYGVHGYLMDSYAMKAATQIALGD
                     NFGYVGYVFVLAFFTNLLLVLFGRYTGAKGIFLTGNTGVSHSQAVLWLIVFWLGFSWT
                     TSIIIAGILTGVFWAFSTTLIVKPIAKVTKDAGFTIAHNQMLGLWFFSKFAHKFGDPE
                     KHDAENLKLPGWLAIFNHNVTAIAIVMTLFVGGFLLSTGIDNVQLMAKGKPWYIYIIN
                     LGLQFSMYMVILLQGVRMMVGEINGSFKGWQDRFIPNAIPAVDVAALLPFSPNAATLG
                     FVFCTFGTIFSMGILLLVHSPIMVLPGFVPLFFSGGPIGVLANRMGGYRSVIICTFLL
                     GIIQTFGTVWAIPLTGLAENGVGWTGIFDWATVWPAICEVLKFIAATFHLGPYAG"
     gene            complement(8658..9785)
                     /gene="rlmC"
                     /locus_tag="RHW33_000456"
     CDS             complement(8658..9785)
                     /gene="rlmC"
                     /locus_tag="RHW33_000456"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV1644004.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQA
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGAVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKI"
     gene            complement(9826..10314)
                     /locus_tag="RHW33_000457"
     CDS             complement(9826..10314)
                     /locus_tag="RHW33_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV1644005.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(10374..11219)
                     /gene="potI"
                     /locus_tag="RHW33_000458"
     CDS             complement(10374..11219)
                     /gene="potI"
                     /locus_tag="RHW33_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV1644006.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(11216..12169)
                     /gene="potH"
                     /locus_tag="RHW33_000459"
     CDS             complement(11216..12169)
                     /gene="potH"
                     /locus_tag="RHW33_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV1644007.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(12179..13312)
                     /gene="potG"
                     /locus_tag="RHW33_000460"
     CDS             complement(12179..13312)
                     /gene="potG"
                     /locus_tag="RHW33_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV1644008.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVAILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(13407..14519)
                     /gene="potF"
                     /locus_tag="RHW33_000461"
     CDS             complement(13407..14519)
                     /gene="potF"
                     /locus_tag="RHW33_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV1644009.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(14870..15346)
                     /locus_tag="RHW33_000462"
     CDS             complement(14870..15346)
                     /locus_tag="RHW33_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV1644010.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(15434..16336)
                     /gene="rimK"
                     /locus_tag="RHW33_000463"
     CDS             complement(15434..16336)
                     /gene="rimK"
                     /locus_tag="RHW33_000463"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV1644011.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(16397..17119)
                     /gene="nfsA"
                     /locus_tag="RHW33_000464"
     CDS             complement(16397..17119)
                     /gene="nfsA"
                     /locus_tag="RHW33_000464"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV1644012.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLNPRLPSSILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(17103..17390)
                     /locus_tag="RHW33_000465"
     CDS             complement(17103..17390)
                     /locus_tag="RHW33_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HDV1644013.1"
                     /translation="MRAIGKLPKSVLILEFIGMMSLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            17550..17807
                     /gene="grxA"
                     /locus_tag="RHW33_000466"
     CDS             17550..17807
                     /gene="grxA"
                     /locus_tag="RHW33_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV1644014.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWMKENLDA"
     gene            complement(17837..18214)
                     /locus_tag="RHW33_000467"
     CDS             complement(17837..18214)
                     /locus_tag="RHW33_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV1644015.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            18484..20169
                     /locus_tag="RHW33_000468"
     CDS             18484..20169
                     /locus_tag="RHW33_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV1644016.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            20502..21053
                     /locus_tag="RHW33_000469"
     CDS             20502..21053
                     /locus_tag="RHW33_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HDV1644017.1"
                     /translation="MDINELKECLHLEVIGKSRKFTWRKVIVRAMKHRRVRYLFWWRI
                     AKYGHEKGGYWRKIAGKIERKILDSYDVKIPLVVDIGKGLDISYLTGVVIGHNVKIGE
                     NCSIKPGVTIGLRGHFDEMDIQIGNNVTIGCNASILGGKVYIGDNVTIGAHALVLHDI
                     PENSIFINKIEYEIIPKKVIAEM"
     gene            complement(21113..21649)
                     /gene="rcdA"
                     /locus_tag="RHW33_000470"
     CDS             complement(21113..21649)
                     /gene="rcdA"
                     /locus_tag="RHW33_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV1644018.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            21733..22941
                     /locus_tag="RHW33_000471"
     CDS             21733..22941
                     /locus_tag="RHW33_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1644019.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            22941..23756
                     /gene="ybjI"
                     /locus_tag="RHW33_000472"
     CDS             22941..23756
                     /gene="ybjI"
                     /locus_tag="RHW33_000472"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV1644020.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKTLHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVASAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            23842..24126
                     /locus_tag="RHW33_000473"
     CDS             23842..24126
                     /locus_tag="RHW33_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644021.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYHPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(24167..25399)
                     /gene="mdfA"
                     /locus_tag="RHW33_000474"
     CDS             complement(24167..25399)
                     /gene="mdfA"
                     /locus_tag="RHW33_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV1644022.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            25684..26280
                     /gene="ybjG"
                     /locus_tag="RHW33_000475"
     CDS             25684..26280
                     /gene="ybjG"
                     /locus_tag="RHW33_000475"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV1644023.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            26338..27096
                     /gene="deoR"
                     /locus_tag="RHW33_000476"
     CDS             26338..27096
                     /gene="deoR"
                     /locus_tag="RHW33_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV1644024.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(27143..28345)
                     /gene="dacC"
                     /locus_tag="RHW33_000477"
     CDS             complement(27143..28345)
                     /gene="dacC"
                     /locus_tag="RHW33_000477"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV1644025.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            28592..29218
                     /gene="gstB"
                     /locus_tag="RHW33_000478"
     CDS             28592..29218
                     /gene="gstB"
                     /locus_tag="RHW33_000478"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV1644026.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(29215..30330)
                     /gene="yliI"
                     /locus_tag="RHW33_000479"
     CDS             complement(29215..30330)
                     /gene="yliI"
                     /locus_tag="RHW33_000479"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV1644027.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFSTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(30441..30824)
                     /gene="bssR"
                     /locus_tag="RHW33_000480"
     CDS             complement(30441..30824)
                     /gene="bssR"
                     /locus_tag="RHW33_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV1644028.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            31037..32362
                     /gene="rimO"
                     /locus_tag="RHW33_000481"
     CDS             31037..32362
                     /gene="rimO"
                     /locus_tag="RHW33_000481"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV1644029.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            32588..32974
                     /locus_tag="RHW33_000482"
     CDS             32588..32974
                     /locus_tag="RHW33_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1644030.1"
                     /translation="MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVI
                     KKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSGLSSKGTKEIEDDELAAYKKMAN
                     AFLAFSNKQIKDLIETGFLIEVKNER"
     gene            32964..33293
                     /locus_tag="RHW33_000483"
     CDS             32964..33293
                     /locus_tag="RHW33_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV1644031.1"
                     /translation="MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAK
                     VKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSKWERNEIKPSGPALRILNTLAIK
                     GPEVFAK"
     gene            complement(33363..34691)
                     /locus_tag="RHW33_000484"
     CDS             complement(33363..34691)
                     /locus_tag="RHW33_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1644032.1"
                     /translation="MSRINKFVLTVSLLIFIMISAIACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(34699..37047)
                     /locus_tag="RHW33_000485"
     CDS             complement(34699..37047)
                     /locus_tag="RHW33_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1644033.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYLLTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(37225..38136)
                     /gene="gsiD"
                     /locus_tag="RHW33_000486"
     CDS             complement(37225..38136)
                     /gene="gsiD"
                     /locus_tag="RHW33_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV1644034.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(38139..39059)
                     /gene="gsiC"
                     /locus_tag="RHW33_000487"
     CDS             complement(38139..39059)
                     /gene="gsiC"
                     /locus_tag="RHW33_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV1644035.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(39077..40615)
                     /gene="gsiB"
                     /locus_tag="RHW33_000488"
     CDS             complement(39077..40615)
                     /gene="gsiB"
                     /locus_tag="RHW33_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV1644036.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(40635..42506)
                     /gene="gsiA"
                     /locus_tag="RHW33_000489"
     CDS             complement(40635..42506)
                     /gene="gsiA"
                     /locus_tag="RHW33_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV1644037.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(42493..43458)
                     /gene="iaaA"
                     /locus_tag="RHW33_000490"
     CDS             complement(42493..43458)
                     /gene="iaaA"
                     /locus_tag="RHW33_000490"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV1644038.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESSLDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            43662..44897
                     /gene="moeA"
                     /locus_tag="RHW33_000491"
     CDS             43662..44897
                     /gene="moeA"
                     /locus_tag="RHW33_000491"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV1644039.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            44897..45646
                     /gene="moeB"
                     /locus_tag="RHW33_000492"
     CDS             44897..45646
                     /gene="moeB"
                     /locus_tag="RHW33_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV1644040.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(45722..46384)
                     /gene="fsa"
                     /locus_tag="RHW33_000493"
     CDS             complement(45722..46384)
                     /gene="fsa"
                     /locus_tag="RHW33_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV1644041.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMAITAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            46515..47414
                     /locus_tag="RHW33_000494"
     CDS             46515..47414
                     /locus_tag="RHW33_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV1644042.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPDVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVDEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            47420..49852
                     /locus_tag="RHW33_000495"
     CDS             47420..49852
                     /locus_tag="RHW33_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV1644043.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELKQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLDAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            49998..50813
                     /gene="ybiV"
                     /locus_tag="RHW33_000496"
     CDS             49998..50813
                     /gene="ybiV"
                     /locus_tag="RHW33_000496"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV1644044.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            50965..52230
                     /locus_tag="RHW33_000497"
     CDS             50965..52230
                     /locus_tag="RHW33_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV1644045.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPQLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(52471..54063)
                     /locus_tag="RHW33_000498"
     CDS             complement(52471..54063)
                     /locus_tag="RHW33_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV1644046.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            54282..55202
                     /gene="ldtB"
                     /locus_tag="RHW33_000499"
     CDS             54282..55202
                     /gene="ldtB"
                     /locus_tag="RHW33_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1644047.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(55261..56379)
                     /locus_tag="RHW33_000500"
     CDS             complement(55261..56379)
                     /locus_tag="RHW33_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV1644048.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLIQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(56376..56843)
                     /gene="mntR"
                     /locus_tag="RHW33_000501"
     CDS             complement(56376..56843)
                     /gene="mntR"
                     /locus_tag="RHW33_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV1644049.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            57029..57157
                     /gene="mntS"
                     /locus_tag="RHW33_000502"
     CDS             57029..57157
                     /gene="mntS"
                     /locus_tag="RHW33_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV1644050.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            57429..59012
                     /gene="opgE"
                     /locus_tag="RHW33_000503"
     CDS             57429..59012
                     /gene="opgE"
                     /locus_tag="RHW33_000503"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV1644051.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(59060..59575)
                     /gene="ompX"
                     /locus_tag="RHW33_000504"
     CDS             complement(59060..59575)
                     /gene="ompX"
                     /locus_tag="RHW33_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV1644052.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(59628..59693)
                     /locus_tag="RHW33_000505"
     CDS             complement(59628..59693)
                     /locus_tag="RHW33_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644053.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            59928..60815
                     /gene="rhtA"
                     /locus_tag="RHW33_000506"
     CDS             59928..60815
                     /gene="rhtA"
                     /locus_tag="RHW33_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV1644054.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            61114..61617
                     /gene="dps"
                     /locus_tag="RHW33_000507"
                     /gene_synonym="pexB"
     CDS             61114..61617
                     /gene="dps"
                     /locus_tag="RHW33_000507"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV1644055.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            62021..62767
                     /gene="glnH"
                     /locus_tag="RHW33_000508"
     CDS             62021..62767
                     /gene="glnH"
                     /locus_tag="RHW33_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV1644056.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            62906..63565
                     /gene="glnP"
                     /locus_tag="RHW33_000509"
     CDS             62906..63565
                     /gene="glnP"
                     /locus_tag="RHW33_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV1644057.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            63562..64284
                     /gene="glnQ"
                     /locus_tag="RHW33_000510"
     CDS             63562..64284
                     /gene="glnQ"
                     /locus_tag="RHW33_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV1644058.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            64545..66770
                     /gene="ybiO"
                     /locus_tag="RHW33_000511"
     CDS             64545..66770
                     /gene="ybiO"
                     /locus_tag="RHW33_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV1644059.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(66767..67693)
                     /gene="rlmF"
                     /locus_tag="RHW33_000512"
     CDS             complement(66767..67693)
                     /gene="rlmF"
                     /locus_tag="RHW33_000512"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV1644060.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     VNASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            67969..68229
                     /locus_tag="RHW33_000513"
     CDS             67969..68229
                     /locus_tag="RHW33_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1644061.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            68494..70776
                     /locus_tag="RHW33_000514"
                     /pseudo
     CDS             68494..70776
                     /locus_tag="RHW33_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            70818..71495
                     /gene="ybiX"
                     /locus_tag="RHW33_000515"
     CDS             70818..71495
                     /gene="ybiX"
                     /locus_tag="RHW33_000515"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV1644062.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            71569..71835
                     /gene="ybiI"
                     /locus_tag="RHW33_000516"
     CDS             71569..71835
                     /gene="ybiI"
                     /locus_tag="RHW33_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV1644063.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            72101..72361
                     /locus_tag="RHW33_000517"
     CDS             72101..72361
                     /locus_tag="RHW33_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1644064.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(72590..73675)
                     /gene="hcxB"
                     /locus_tag="RHW33_000518"
     CDS             complement(72590..73675)
                     /gene="hcxB"
                     /locus_tag="RHW33_000518"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV1644065.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDGGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(73816..74778)
                     /gene="ybiB"
                     /locus_tag="RHW33_000519"
     CDS             complement(73816..74778)
                     /gene="ybiB"
                     /locus_tag="RHW33_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV1644066.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(74806..76956)
                     /gene="dinG"
                     /locus_tag="RHW33_000520"
     CDS             complement(74806..76956)
                     /gene="dinG"
                     /locus_tag="RHW33_000520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV1644067.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLTQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            complement(77240..>77994)
                     /locus_tag="RHW33_000521"
     CDS             complement(77240..>77994)
                     /locus_tag="RHW33_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDV1644068.1"
                     /translation="MRLSQQALISMLEPEVKKKTIWNNYSIYPSLQDTHEIVIDDPET
                     ICMRAFPLFAKGWEYAQKNKKHQLILNALGFKGYIRDIFMSAIMRKTDFVPECNNQPT
                     ELNSSFSSLINDSDQWQQHTLKDKHYASLLTMLDLNEASERDKSKIFFCLSAVFANIS
                     HSSVFNGIPDASKTLKRYAFALLVKAHSLDESMISNQTFNIYKTVLLDLNNLSNEEAN
                     QLRISSLYRDMVRYAQYRFSKVLSEWTPDAWV"
BASE COUNT        19612 a        20441 c        18933 g        19008 t
ORIGIN      
        1 tgtagcataa ataatattat caaaagtcaa agctgggact atcaaatttc gctcattttc
       61 aaaaatacaa cataattttg gcaatcatct taatagcagg atctcttatc gcatgtgaaa
      121 aatttgtaat gattagacaa taaccgcgtt catttatcac gcgttataaa tgtgaaactg
      181 gcttcttgca gatgctctta acctctacag gaggatttat accaccattt cagaactatc
      241 atatttacat taaatgatca tttcagccat ataacacgct caacctcaca aatggtttac
      301 actgtctatt gtttgggtaa tttaagtaag tcagctacga gcaaccttga gtgggtagcc
      361 agcaatggac tcaaacccaa tcaggtgttc agattgtttc agaattgtat taaaaacaat
      421 gtattaaata atctgatagc gtttggtcgt agtgtgacgc agacagcaag ctaataagga
      481 aatgattatg cgctaccgtt cactatctct acttattcca tgtgcactgc tgctgagtgc
      541 ctgtaccacc gtcacgccag cttataaaga taacggtccc cgcaccggtt cctgtgtcca
      601 gggggggcct gacagcgtgg cccaacaatt ctatgactac cgcattcaac atcgcagcaa
      661 tgacattacc gccctgcgcc cctatttgag tgacaaactg gcaaccctac ttagcgatgc
      721 cagccgcaat aatagtcatc gtgaattgtt aagcagcgat ccgttctcca gtcgaaccac
      781 cttgccagac agcgcccacg ttgccagtgc atcgactatc cctaatcgtg atgcccggaa
      841 cattccattg cgcgtggatc tcaagcaggg tgaccagggc tggcaggatg aagtgttaat
      901 gattcaggaa ggccagtgtt gggtgatcga cgatgtgcgc tacttaggcg gtagcgttca
      961 tgccacggcg ggtacgctcc gtcagtccat cgagaatcgc taacgccttg atcaaccaat
     1021 aaaccccgta aattgtccgg catttcgtga ggaatgccga catttatgct cgccgaccac
     1081 cgcccccgtt attttgtgct atgtttattg aataatgcgc tttgctttta acttttaaag
     1141 cagaaatatt gcataattat tctgtcaaag gtactatctt cgacctcaat agctatcaga
     1201 ctgccagtat acgagtgtca atgagtattc aattaaacgg cattaattgc ttctacggcg
     1261 cgcatcaggc gctgttcgat atcacgctgg attgcccaca gggcgaaacg ctggtgttac
     1321 ttggccccag cggcgcgggt aaaagctcgc tgctgcgtgt actcaatctg cttgagatgc
     1381 cgcgctccgg tacgctcaac attgcaggca accatttcga tttcaccaaa acaccctcag
     1441 acaaagcgat tcgcgatttg cgtcgtaacg ttggcatggt gtttcagcaa tacaacctgt
     1501 ggccgcatct gaccgtgcag caaaacctga ttgaagcccc ctgccgtgta ctggggttga
     1561 gtaaagatca ggcgctggcc cgtgcagaaa aactgctgga acgtctgcgt ctcaaacctt
     1621 atagcgatcg ttacccgctg catctttctg gtggtcagca gcagcgtgtt gctattgccc
     1681 gtgcgttaat gatggaaccg caggtactgc tgtttgatga accgaccgcc gcactggacc
     1741 cggaaattac cgcacaaatc gtcagcatca ttcgtgagct ggcagaaacg aatattaccc
     1801 aggtgatcgt cacccacgaa gttgaagtgg cgcgtaaaac cgccagccga gtggtgtata
     1861 tggaaaatgg tcatatcgta gaacaaggcg acgcgagctg ctttaccgag ccgcaaaccg
     1921 aagcatttaa aaactatctc tctcactaag attcgggata acaacaatga aaaaagttct
     1981 gattgccgcg ttaattgcag gttttagtct ttccgccaca gctgccgaaa ccattcgttt
     2041 tgctaccgaa gcctcctatc ctccgtttga atcgattgat gcaaacaacc agatcgttgg
     2101 ttttgacgtc gacctggcac aggcgctgtg taaagagatt gatgcgacct gtactttctc
     2161 taaccaggca tttgacagcc tgatcccaag cctgaaattc cgtcgcgtgg aagcggtgat
     2221 ggccgggatg gatatcaccc cagaacgtga aaagcaggtg ctgtttacca ccccgtacta
     2281 tgacaactct gccctgtttg tgggtcagca aggcaaatac accagtgttg atcagctgaa
     2341 aggcaaaaaa gtcggcgtac agaacgggac gacacaccag aaattcatta tggataagca
     2401 cccggaaatc actaccgttc cgtatgacag ctaccagaac gcaaaactgg atctgcaaaa
     2461 cgggcgtatc gacggcgtct tcggtgacac cgcagtggtc actgagtggc tgaaagataa
     2521 cccgaaactg gcggcggtgg gcgacaaagt gaccgataaa gattacttcg gcactggcct
     2581 cggcatcgcg gtacgtcagg gcaacactga actgcagcag aaactcaaca ctgcgctgga
     2641 aaaagtgaag aaagatggca cttacgaaac catctacaac aaatggttcc agaagtaatt
     2701 cctgatgaat gaattttttc ctttagcaag cgccgccggg atgaccgtcg gccttgccgt
     2761 ttgtgcattg attgtcggac tggcgctggc gctgttcttt gcggtgtggg agtcggcaaa
     2821 atggcgtcct gtcgcgtggg caggttcagc gctggtaacc attctgcgtg gcctgccaga
     2881 aattctggtg gtgctgttta tctattttgg ttcctcgcag ctgctgctga cgctttcgga
     2941 tggcttcact atcaatcttg ggttcgtgca gatcccggtg cagatggaca ttgagaactt
     3001 cgacgtgagt ccgttccttt gtggtgtcat cgctctgtca ctgctgtatg ccgcctatgc
     3061 ctcgcaaacg ctgcgcggcg cgttgaaagc ggtgccgatg ggtcagtggg aatccggcca
     3121 ggcgctgggg ctgtcgaaat cggctatctt tttccgtctg gtgatgccgc agatgtggcg
     3181 tcatgcgctg cctggcctcg gtaaccagtg gctggtgctg ctgaaagata ccgcgctggt
     3241 cagtttgatt agtgtgaatg atttaatgct acaaaccaaa agcatcgcta cccgtaccca
     3301 ggaaccattt acctggtaca ttgtggcggc ggcgatttac ctggtgatca ccctgttgag
     3361 tcagtacatt ctcaaacgca ttgacctgcg cgcgacacgt tttgagcgga ggcccagcta
     3421 atgtttgagt atttacccga actgatgaaa gggctgcaca ccagcctgac gctgacggtt
     3481 gcctcgctga ttgtggcgct gattctggcg ttgattttta ccatcatcct gacgctgaaa
     3541 acgccggtgc tggtgtggct ggtgcggggt tatatcacac tgtttaccgg tacgccgctg
     3601 ctggtgcaga tcttcctgat ttattacggg ccgggccagt ttccgacgtt gcaggagtat
     3661 ccggcactgt ggcatttgtt gtcagaaccg tggttatgtg cgctgattgc gttatcgctg
     3721 aacagtgcgg cgtataccac gcagctgttt tacggtgcaa ttcgtgcgat cccagaaggt
     3781 cagtggcagt cctgtagcgc cctgggaatg agcaaaaaag atacgctggc gatcctgctg
     3841 ccgtatgcct ttaaacgctc gctctcttct tattccaacg aagtggtgct ggtgttcaaa
     3901 agtacctctc tggcttacac cattacgctg atggaagtga tgggatacag ccagttgttg
     3961 tacggacgca cctacgatgt aatggtgttc ggtgcggcag ggattattta cctggtcgtc
     4021 aacggcctgc tgacgctgat gatgcgtctg atcgagcgca aagcactggc atttgaacgg
     4081 cggaattaat gtagctgcgt gctgatgccg gatgcggcgt aaacgcctta tccggcctac
     4141 attagctgga tgatttgtag gtctgataag acgcgccagc gtcgcatcag acatcagcac
     4201 agtgcctgta ccggatgcgg cgtggacgcc ttatccggcc tacatagtgc ataaattctt
     4261 ataataaaga cggacaactc actaagttgt ccgtcttttt tcatttcatg caaattattt
     4321 aatcatattt attgcatata aattcacttg atggcattgt tatcccatgc cgcagacacg
     4381 gccaataatc ataagattga cagacgggag ttccatcatg aaaaagttag ttcttgccgc
     4441 attactggca tcctgtgcgt tcggcgcttc tgccgcagag aaaatcaatt ttggcgtatc
     4501 agcgacctat ccgccgttcg agtcgatgga tgccaataac cagatcgtcg gctttgatat
     4561 cgacctggca aaagcgttat gcaaacagat gcagacagaa tgtactttta ccaatcacgc
     4621 gttcgacagc ctgatcccgt cactgaaatt caggaaatat gacgccgtta tctccggtat
     4681 ggatatcacg ccagatcgta gcaagcaggt atcgtttacc acgccctatt acgaaaactc
     4741 agccgtcgtg attgccaaaa aagataccta caaaacgttt gccgacctga aaggcaaacg
     4801 cattgggatg gaaaacggta ctacgcacca gaaatatatt caggatcagc acccggaagt
     4861 gaaaacggtc tcctatgaca gttatcagaa tgcctttatc gatctgaaaa atggccgtat
     4921 tgatggggta tttggtgaca cagcggtggt taatgaatgg ctgaaaacca acccgcaact
     4981 gggcgttgcg actgaaaaag tgaccgatcc gcaatacttc ggcaccggtc tgggcatcgc
     5041 tgtacgcccg gataacaagg ccttgctgga aaaactgaat aacgcgctgg cagcaattaa
     5101 agctgacggt acatatcaga aaatcagcga tcagtggttc ccgcaataat tattcatttg
     5161 ccggagtggg cgaacaccca ctccggcaaa atcacttctc tacgctaatc ccaggcgctg
     5221 aaactgctct ggcggcgcat cattatcttt catcaactga atcagcaacg cgatcatccg
     5281 ggactcgatt acatcatcat gaatcggatg tagctgctgc ggatcgtttt gcagatcaaa
     5341 cagcaaatga ccaaactgat tcaacccctt atatttttcc tgtgccggga tgcgtaatac
     5401 ctgtaaccct ttggtaaaac gaaacggtgg ggccagcgat aaagcccgca tctcatccac
     5461 cggatagcgg ctgttcattt tgcaggacat caacgtgtag ttggcaatat catcctcacg
     5521 ccccggcagc gctgcacgca tatacacata acgcccatca gttacgttag catgaccact
     5581 gaaaacacca aacatcgccg agctgcgtac tggttcatcg tgactgaccg ttttcgccag
     5641 cggttgcccc tgcacatcgg gcggcactgg ctgttgaaaa aagtcataca acgttggcgc
     5701 ccagtcgatc atttgtacca gtgcctgtcg ccgctctcct tttacaccac tgcgcgggtc
     5761 ccagataaac aacggattat tggcaacttc gttgtaataa ggcatctggt ttttcgccca
     5821 ccagccatgc tcaccgagta aaaagccgtg gtcagtgccg acaatcagca tggtatctcg
     5881 ccacagatcg tgctcatcca tcaggtcgag aatgcgcccc aggttgcgat cgcacatcga
     5941 caccaacgaa cgataccggc aacggatgtg ctccacggcc tcgtcgctct cctgcacccg
     6001 gccacgcggc cagtcataat gcggtccatc ccagttatct tcatacaagg aaagatactc
     6061 ttccgttgtg tgaaacggct cgtgcggatc aaacgtttcg acctgcaata accagttatc
     6121 ttccgcgtga tttttgtgga tgaaggtaca gcctgcatca aacactttgg tttgtggaaa
     6181 atccgcttcc tgctgaatat attcccggtt tgcccagtca tggcgccaca gaccagaaac
     6241 accgccaccg gtctgctttt gcggcacccg tagcacctcg ggaataggcg gttgcgcaac
     6301 gcttgcatgc cagtgatcgc cttcctgtcc gcgcacaatc tcccacgaac tgtagcgatt
     6361 atgataattc ccgccgccat cttcccagta atgcaaatga tcgcttatca ggtgggtata
     6421 gatccccgcc tttttaagca gctccggcat ggaatcgtca aacggttcta acggccccca
     6481 ctcccgatgc aggaagttac atcgcccggt atgcaactcc cgccgggcag gcatacacgg
     6541 catactgccg acgtaactgt tttcaaaggt ggcggcatga gccgccagac gttggaaatt
     6601 aggcgctttg gttaacgcat cgccataagg tggcagataa cgtttattca gactatcgaa
     6661 cagcagaata atcgctctca ttacgcccta ccttattgat taattaaatg cggataatgt
     6721 tgctgaacca cttcataaat ttgctgacca atagtttcac cgtccataat attctctatc
     6781 gggatcagat attgcccttg tttcgggtca agactaacca catcgagtaa ttttttggaa
     6841 atagcaaaaa tatccgccca gtcggtaaat ccgccgactt cacctgcggc ggtatgttcc
     6901 atttcaactt tgaaatcata tttggtcagc gcttttttaa cgaccgtttg taacataacg
     6961 ctactgccta caccgtaacc acagacaaac agaatattaa ttgtatcttt catttttaaa
     7021 cccttaatat ttaattaacc cgcgtaagga ccaagatgga atgttgcagc gataaatttc
     7081 agaacttcac aaatagcagg ccatacggtt gcccagtcaa atattcctgt ccagccgacg
     7141 ccattttcag caagcccggt taacggaata gcccacaccg taccgaaggt ctgaataata
     7201 cccagcaaga acgtacagat aattacggaa cggtatccgc ccatgcggtt tgctaatacg
     7261 ccaattggac cgccagagaa aaacagaggt acaaagccag gcaataccat aattgggctg
     7321 tgaaccagta ataagatccc catcgaaaaa atggtgccaa aagtacagaa gacgaagcct
     7381 aacgttgcgg cattgggtga aaaaggtaac agcgctgcaa catcgacagc aggaatagcg
     7441 ttaggaataa agcgatcttg ccagccttta aacgaaccgt taatttcgcc caccatcatg
     7501 cgcacaccct gcagcagaat gaccatatac atggagaact gtaagccaag gttgatgata
     7561 tagatgtacc agggcttgcc tttcgccatt aactggacat tatcgatacc cgttgatagc
     7621 aagaaaccac cgacaaacag cgtcatcaca atggcgatag ccgttacgtt atggttaaaa
     7681 atcgccagcc acccggggag tttcaggttt tcagcatcgt gcttttcggg gtcaccaaat
     7741 ttatgggcga atttggagaa aaaccacaat cccaacatct ggttatgagc aatggtaaaa
     7801 ccggcatctt tggtaacttt ggcaatgggc ttaacaatga gcgtggtgga gaacgcccag
     7861 aatacacccg ttaatattcc ggcaataata atggatgtag tccagctaaa acccagccaa
     7921 aagacaatca accataatac cgcctgagaa tgtgaaacac cggtattccc tgtcaaaaat
     7981 atccctttgg cacccgtata ccgaccaaat aataccagca gcaggttggt aaaaaacgcc
     8041 agtacaaata catacccgac ataaccaaaa ttatccccca atgcaatttg cgttgccgct
     8101 ttcattgcgt aggaatccat taaatatccg tgaacaccat aggctttact tacggcttct
     8161 gctaccggtt taaatgttgc ggaaaactgg aaaccaccaa ataacaccat cattaatccc
     8221 accatggcgc tgaccatgcc ggtaataatt tttattgacg atttcttatc aagggcataa
     8281 ccaataccca ccagcaaacc aattaaaatt gccggttcag ttaaaaacgt ttgaataaca
     8341 tctatgaatt tggcaaacat cgtcggaacg ccctccataa gcgtacattt ccgttgtcaa
     8401 tgcatgaacc ttctgctgac gttaataatg tctcaggaaa ttttgtgttt cgctaagcaa
     8461 tgcctctgta tgtattcggt aagtgcactg tagtaatcaa aacagttcaa ttaaagatct
     8521 tatttgatta tttgtgattt gatttgcaaa aatagagcaa tatataaccg tgatcgatcg
     8581 gttattatta atcgatcgat cacgattatt attttgagtg aatagttgtt gccgaaaaat
     8641 ttactgccgt gatgtattta aatttttacc agcagtgtca acacttcata atgcgcggtg
     8701 tgcgggaaca tatcgaaaag ctgcacccgt tcaatgcgat aaccaggcag ttctcggata
     8761 tctttcgcca tggtttgggc gttacagctg gagtagatga taaaacgcgg tgccatcgtt
     8821 gagagataat cacacagcgg tttaccaatg ccgcggcgcg gtgggttaac cagcaccagc
     8881 tccggcaccg ccccctgagc ggtggcaaac tgagtggagt ccagcgcctg aaattgcaga
     8941 cgcgttaagc ccagttcagc ggctgactgc tttgcacagg caatggcctc tggcgcaatt
     9001 tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta aaccaaagcc ccccacaccg
     9061 cagaacagat cccacatatg tttaaccggc agctgtcgta cccagtcgcg cgcggtagcg
     9121 tacagctggc tggcgaccgc cggattagtc tgaaagaaac tttgcggacg gatccacagc
     9181 ggcacatcgt taaaacgctc tgccagcgcc tgttgttcgg tcaggtagat ctccgtctcc
     9241 ccttccataa tcgccatatg taccggctga atattgacgg tgataacttt tagctgcggc
     9301 aattgtgcct gtaaccacgg caatgcctta cgcagttgtg ccagtttggt ttcagaacgc
     9361 agtacaaagc gcagcatcat gccgccatcg ctctggcttt cagtcagcag aatgtatttc
     9421 agttcgccac gcttacgcgc cacgttgtag ggcgttaacc ccgctcgggc gataaacggt
     9481 tttagcgccg caaaaacggg cgcaaatgag gcaggataaa gcgggcagtc acaaaggtct
     9541 tccggtgtgc cgtcgcgatg cagcataccg agcagtggtt tttcaacgct accactcacc
     9601 accattttgg ctttattacg aaacccttgt tccgggcctg acaccggcgc gcaccattcc
     9661 tcaaccggaa agtcggcgag cagattttta agatcggcgg ttttagcgga gagttgctct
     9721 ggaatcggct gcgttatcca ctgacaggaa cgacagcgac ccgcgtcgta aagtgcgcac
     9781 tgcatacatt gaccttcaca tcatcagggg cgacgattat acacattatt gcaactggaa
     9841 gaaccgccga ctggtcgagg gaacgaacag cagcatcagg atgagcatat ccggcagctt
     9901 ctgcagcatc aggctatgga agatttcacg ttttgattca ccgggaatgc tgaacagctc
     9961 cggataaccg taccccagcg aagctgccca caagtaactt gcggccgtga tttgtgtcag
    10021 cagatatagc catcgcgccc agcgacgccc tttcaccagt gaaaacgcac accagatctc
    10081 aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt aaactccagg tttgtacgct
    10141 gcgatgaatg aactcgccaa taccgcgcac gcccagcgta ttgaaaatca tcagcacgtc
    10201 gaggccacgg atcataataa tggcgagcgc cgccacctgc accagcgcag gcacattcag
    10261 gcgagcatga gatgaagatg ttttcttaaa aaatcccaac gtttcgtctt ccatgaaaac
    10321 aatgccgcga catgcgcggc attatgtagc caggttggca aattttagtg tcttcagcca
    10381 cgtcttgcac gctggatgtc gcgtatccgc tgtttttctg cgcgagccat cagataccag
    10441 gcgataaatc cgacaattcc gaccgcgccg aggatcaatg ttgccagggc gttgatttcc
    10501 ggattcaccc ccatccgcac gctggaaaag accagcatcg gtaacgtggt ggctcccggc
    10561 ccggaaacaa agctggcgat caccagatca tcaagcgaca aagtaaaagc cagtaaccag
    10621 ccagaaatga tcgcgggcat gatcatcggt agcgtaataa cgaaaaacac tttcagcggc
    10681 gtcgcaccga gatccatcgc tgcttcttct atcgagcgat ccagttcccg cagacgcgac
    10741 gaaataacga ctgccacata agccgtacag aaagtgacat gcgccagcca gatggtaagc
    10801 ataccgcggt ccgcaggcca gccaatggca tgagcaagcg cgacgaataa taacaacagc
    10861 gacaagcccg tgatgacatc tggcatcacc agcggcgcgg tgatcataaa ggcaaagcca
    10921 tttgatccgc gaaacctgcc aaagcgcacc agcaccaccg ccgcaatagt tccgaggatc
    10981 gccgccgccg tcgccgcaca ggccgcaatt gtcagactta aaccaaccgc actcatcatc
    11041 gcgtcatcgc gcaataactc accataccag cgcgttgacc agccggccca caccgtcacc
    11101 agcttcgagc tgttaaacga atagataacc agcatcagca ttggcgcgta gagaaaggta
    11161 aagcccagca gcaaaatcac aatccgccag ggcgaacgaa ctaccggtaa attattcatc
    11221 cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca cattatcggc acaattagca
    11281 gcagcaacat gatgatcgct accgccgagg ccaccggcca gtcgcggtta ttgaaaaact
    11341 cttgccatag cacgcgcccg atcatgatgc tgtccgggcc accgagcagt tccgggatca
    11401 caaactcgcc cacagccggg ataaacacca gcatcgatcc ggcaataatc ccacctttag
    11461 tcagcggcac gatcacagta aagaacgttt tcagcggtcg tgcaccgaga tccagcgctg
    11521 cttccaccag cgaataatca atacgaatca acgcggtata aatcggcagc accataaacg
    11581 gcacgtaggc gtaaacaatg ccaatataaa ctgccagatt ggtatgaaga atggtaagcg
    11641 gttgatcgat aacccccagc cacagcagaa aattattcag cacaccgttg ttttttaata
    11701 ttcccatcca ggcgtagaca cgaatcagaa acgaagtcca cgacggcagg atcaccagta
    11761 gtaataagat attacgggtc gaaggcttac tgtgcgccac cgcccacgcc agcggatagc
    11821 cgatcagtaa acagcaaata gtcgaaatcg ccgccacctg taacgactgg agataagcat
    11881 caaaatagag cggatcgtcg gtcagttgca gaaaattacc gagattaaga gtgatggaaa
    11941 gttgcccgtc agcccactcc atcagttcgg tataaggtgg aatagcgcgc gccatctctg
    12001 ccaggcttat tttaaaaaca atcagaaatg gcagcagaaa cagcaagatc aaccagatat
    12061 atggcaacgc aatgaccagt ttgcgcccat gcttcatttg cagctgcgac aaccacagct
    12121 taaatccgcc cggcggtttc gactgggcag caggttcaag tgtactcatc tttgctcctt
    12181 aaaccgtcag caccacacag ctgtccactt cccagcacaa acgcacttcg tcgccccagg
    12241 tcggtaaccc tttacgatga cgatgggcgt tttgtagctg ggcgctaatc atctgcccac
    12301 ttttcagacg aacgtgatac accgaaagat cgccgagata ggcaatgtgt atcacctccc
    12361 ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca aagcatgatt ttttccggac
    12421 gcagcgctac gtgtaccggc acgttatcga ccactgaagc atccgcatcg actttcagtg
    12481 gatgcaccag ccccggcgaa taaagcacca gaccatcttc ctgacgctct ttgagtacac
    12541 cttcaaagac attaaccgag ccaataaatt cggcgctata gcgggtagtc ggatgctcgt
    12601 agatctcttc cggttcgcca atctggacaa atttcccacg attcataatg gcgatgcgcc
    12661 ccgccatggt catcgcctct tcctgatcgt gggtgaccat cacacaagtc acaccgacgc
    12721 gctccagaat agccaccact tcaagctgca tccggtcacg cagcttttta tccagcgcgc
    12781 ccatcggctc gtcgagcagt aatagtttcg ggcgtttggc aaggcttcgg gccagggcca
    12841 cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg tttggcgaac tcctgcatat
    12901 gcacaaggcc gagcatctca ttgacccgac tggcaatttc cgctttcggt agtttgtcct
    12961 gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg cggaaacagc gcgtaagact
    13021 gaaacatcat attgataggg cgcaggtaag gcggtacctg tgacaaatcg acgccatcga
    13081 gcataatctg cccggcagaa ggttgttcga aacctgccag catacgcagc agcgtggact
    13141 tgccacagcc ggatgcgccc agcagcgcga agatttcacc tttgtagatg gtcaggctga
    13201 catcatccac cgcatgttga ccatcgtaag atttggtgag gttgcggatt tctaatagcg
    13261 gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat agcgtcattc acggctgctc
    13321 tccggcattg aatgtgtgca agcgcaccat aatggtgcga attgccggcg ggtgtggtgc
    13381 gcccgtccgg catctacggc tgcggattat tttccgctct tcactttggt ccacgcgcgg
    13441 gtgcgcacac ggtcgatttt cgggtcctgc actttcagag tgaacaactt cgcacggaca
    13501 tccgcaggcg gataaatacc cggattatca cggacttccg cacttaccag cggcgtagct
    13561 tctttgttcg cattggcata gaacacatgg tcggaaatat gcgctactac atccgggcgc
    13621 agcaggtaat tcaggaactg ataggcttcg tctttgtttt tggcatcggc aggcatggca
    13681 aagacatcaa agaacgccat cgccccttct ttagggatcg agaacgagac attcacgcca
    13741 ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat cacctgccca gccgatagcg
    13801 acgcaaatat cgccgtttgc caggtcgtta atgtattgag atgaatggaa ataacgaatg
    13861 ttcgggcgca gctttaacag cagatcggta gccggtccgg tgtaatcatc cgctttagtg
    13921 ctgttgggat ctttgccgag ataattcagc acggtagcaa aaacttcttc tggcgcatcg
    13981 aggaaagata caccgcagct tttcagtttt tccagatttt caggcttgag gatcaagtcc
    14041 cagctatcga cgggcgcgtt ttcgcccagc accgctttga ctttatcgac gttatagcca
    14101 atcccggtcg tcgcccacat atagggcata gcaaatttat tgtcgggatc gtgtttggcg
    14161 accaacttca gcagttccgg gtcgagattc ttccactccg gcaatttgct tttatccagc
    14221 ggctggaaaa ctcccgctgt cagctggcgt tcaagaaagc ttgctgacgg caccaccagg
    14281 tcaaagccgg tgctcccggc cattaatttg ccttccagta cttcgttaga gtcgaaaaca
    14341 tcgtagacga ctttaatacc ggtttctttt tcaaagttag ccaccgtgtc gggggcgata
    14401 taatcagacc agttataaat gtggagtgtt ttttgttcag ccgcgagcgt gccgacagag
    14461 acggccatca gagcacccgc aaccagaccc gaaagccatt ttttatttaa ggcggtcata
    14521 tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag ttgtcacact gaaagatatg
    14581 caataaacgt gcatattttt tatcggcgcg gtgtaagaaa agagaactct ctcccggcca
    14641 gcactgctcg ctatataaaa gcatcgcaag aataacttta gccagggttt gagactatag
    14701 ctcggattaa aaaacggtat ttaacaaatt tttatgcgta tttgctctat gtgataaagc
    14761 caaatcgaca caagagaagt gaaataatct ttaagaaaag gttaaaaaca gcagataatt
    14821 ttgcagcgcg cagccactgc accagcgact gcgtgctcga aaggagttat cagtggagga
    14881 aatagttttc ggtaacgttg ttttgccctt catcgtcagt cggtaacagc agttgatggg
    14941 cattagcttc aagaatgacc atcgaaatct gctcttcgct ctgacgcacg aaccatgcaa
    15001 actgctcata ggttacgccc tgcataacgg ataaagactg gcaaactacc agctttggca
    15061 gattatcatc ctgcatatca agaaatgctt tcacggtcag cgaactggca ttgatggcag
    15121 ataaatccgc cgccagcggc aatacggctg aaggtcggac ttccgccatg gcagaaaaca
    15181 ggatcgtgtt atcgatcaga tcgattttgg catcaaagac accgtcgaaa ttctgcatat
    15241 ggggcagatg cagagcctga cagttatcac attcaaaaaa actcatcccc aggtcatcga
    15301 gccattgacg cagcgtatcc agaccaggaa cgaccagcga tgtcataatt ttgtacaacc
    15361 tctttcgata aaaagaccgg cacagcttac gcaaaaagcg caggcaaaac catgatcagt
    15421 aatgtgattg cgactaacca cccgttttca ggcaatattc tgtcgtagcg tggcgttcga
    15481 tccagcggat cattttaccc gcgatgtcga taccggtggt tttttctatt ccttccagcc
    15541 ccggcgacgc attcacttcc atcaccaacg gcccgcgatt agcacgcaga atatccacac
    15601 cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc gatttcacgc tcctgtggtg
    15661 tgatactggc gacgcttgcc gcgccgccac gatgcaaatt ggaacgaaaa tcgccctctt
    15721 tcgcccgccg ttcaatcgca gcgaccactt catcgccaac aaccagacag cggatatcgc
    15781 acccttgcgc ctctttgata tattcctgca ccagaatatg cgcgttcaga ccacggaaag
    15841 cgtcaatcac gctttccgcc gcctgacgcg tctccgccag cacgacgcca attccctgcg
    15901 tgccttcaac caacttgacc accagcggcg caccaccgac catgtcgatt aaatcgctgg
    15961 tatcatccgg cgaatgcgca atgcccgtga caggcaggtc gatgccctga cgcgccagca
    16021 gttgcatgga acgcaatttg tcacgcgccc gggcaatggc gaccgactca ttgagcggat
    16081 agctccccag catctcgaac tggcgcagtg ccgccgtccc ataaaaggta atggcggtgc
    16141 caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg gcctttgtag tgaatagaag
    16201 acgccgcagg atttatgttc atatagcaag aaagcggatc aagaatttca accaggtgac
    16261 cgcgctgtat agcggcttca cgcagccgct tacacgaata gagcgttcca tcccgggaca
    16321 atatggcaat tttcaccctg cacctctctg tcagacctgg tcaaaagccc gcgtatcata
    16381 catcgacgtg gcggttttag cgcgtcgccc aaccctgttt gtgcaaataa tccagaataa
    16441 atgggcggct ttctttaatg attgttcggc ggatatgatc gctccaggta tcccggcgat
    16501 tattgctgcc acgggtgagg taatattccg ccagttgctc gtcatactgc gccagtgcgt
    16561 ctttatccag cggttgatag ctgttttcat gcaccaaaat ggaggacggt aaacgcggat
    16621 taagatccgg attatccgca ggccagccaa ggcacagccc aaacagcggc agaacatgct
    16681 gcggtaattt cagcagttcc gtcaccgctt caatattatt gcgcaggccg ccgatatata
    16741 cgccgcccaa tcccagcgac tccgctgcgg ttaatgcatt ctgcgccatc attgccgtat
    16801 caacgacacc gagcaacagc tgttccgcca ggccgagctg agcatccgga cagatctgta
    16861 aatggcggtt aaagtcggca cagaacaccc agaactccgc cgcttgcgct acgtgttttt
    16921 gcccgccggt cagcgtcacc agttcttcac gtaacgcttt gtcggtaatg cgaataatgc
    16981 tactgcactg caaaaaacta gaactggacg tcgcacgggc gctgttaata atcgcctcac
    17041 gctgcgcttc ggaaatgggt tcatcagtga aatggcgaat ggagcgatgg ccacaaataa
    17101 gttcaatggt tggcgtcatt atctttttct ctttctgaac gtgaatattg cggtggacgg
    17161 ttcatcagct gtggggcaag acgttttgcc acctgaagaa taaccaccac cgcagcggga
    17221 agcatgagca aaacaccgag aaaaatcatc agaatctgca cttctggccg agaaaatggc
    17281 tcaggcagcg acagggtgtc gcttaccgac agcagcgcca ccgccagcga catcattccg
    17341 ataaattcca gtatcaacac gcttttaggc aatttaccga tcgcgcgcat acgcttccct
    17401 ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt gtgtttaaca gttatagctt
    17461 ttagcaatta atgcaacagg ttaaacctac tttcagcgaa tacattttag cgtgatcatt
    17521 acaggcataa atctatgagg agagaaataa tgcaaaccgt tatttttggt cgtccgggtt
    17581 gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt gagcaatgaa cgcgatgatt
    17641 ttcagtatca gtatgtagat attcgtgcgg aagggatcac taaagaagat ctacaacaaa
    17701 aggcaggtaa acccgtagaa accgtgccgc agatttttgt cgatcagcaa catatcggcg
    17761 gctataccga ttttgctgca tggatgaaag aaaatctgga cgcctgatcg tctgacaagc
    17821 cctcgcgttg agggctttac tgattttttc tgtgctgtgg tttaaacaaa ctactgataa
    17881 ataagaaaca cagtgccccc agcgcacacc agaacaccgc gcttagtaac catgccagct
    17941 cttgccagaa tgagcgggtc ggtgaaaaaa acagccgcat aataagcatt gaacagggtg
    18001 ccgccagcat tgcgccaaac agaggtttca ggacttctct acgctgtgaa aagaaactgg
    18061 caactgctcc aggaagaatg aaaaacagca agccgatttc aggatgcccg gcagcccgaa
    18121 aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac aatgaagagg acaaaacagc
    18181 agattgcccc cgcccaacgt tgtttatgtt tcactcgttc ctcctgacac tgcgtctatc
    18241 gaacacattt ttcgccagtg tggcgttcag taagataaag ccgcttcgca ttccatgcta
    18301 atataggcca acgcaattca tatagccgtt gatacctaat gtgattacac tagtaaaata
    18361 tattgttact ttactatcgt ttaggtgcgc tgaatgaatc tgcgccctga attctggtaa
    18421 aaaacattat cgtaaattac catttctttc aacagcttac tagtaaacaa gaagttagcc
    18481 tccgtgaata taaacgtcgc cgaattgtta aatgggaatt acattctgtt attatttgtg
    18541 gtcctcgcgc ttgggctatg tctcggaaag ttacgacttg gttcgatcca actgggtaat
    18601 tccattggcg ttttagtcgt atcgctgtta ttaggccaac aacatttcag cattaacacc
    18661 gatgcgctta atcttggctt tatgctgttt attttctgcg tcggggtcga agccggaccg
    18721 aacttttttt ccattttttt tcgcgatggg aaaaattacc taatgttagc actggtgatg
    18781 gttggcagtg cgctggtgat cgccttaggg ttaggtaagc tgtttggctg ggatattggc
    18841 ctgacggccg gtatgttagc aggctctatg acgtcgacac cggttctggt cggtgctggc
    18901 gatacactgc gtcattccgg catggaaagc aggcagctct cactggcact ggataatctg
    18961 agcctcgggt atgccttaac ctatttaatc ggtctggtga gtttgattgt tggtgcgcgt
    19021 tacttgccga aattgcagca tcaggactta cagaccagcg cccagcaaat cgcccgcgaa
    19081 cgtggcctgg acactgatgc caaccgtaag gtttatttac cggtgatccg cgcctatcgc
    19141 gtcggcccgg agctggtggc ctggaccgac ggcaaaaatc tgcgtgaact gggtatttat
    19201 cgacaaaccg gctgctacat tgaacgtatt cgacgtaacg ggattctggc aaatccagac
    19261 ggtgatgccg tgctacaaat gggcgatgaa atagcgttgg taggctatcc cgacgcccat
    19321 gcccgactcg atcccagctt ccgtaacggt aaagaagttt tcgatcgtga ccttctcgac
    19381 atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata acgctgtagg taaacgtctc
    19441 gcacaactga agttgaccga tcacggttgc ttccttaacc gcgtcattcg tagccagatt
    19501 gagatgccga ttgatgacaa cgtcgtgctt aacaaaggtg acgttttaca agtcagcggc
    19561 gatgcccgcc gcgtaaaaac catcgccgat cgcatcggct ttatctcgat tcacagccag
    19621 gtcactgacc tgctggcatt ctgcgccttc tttgttattg ggctgatgat cgggatgatc
    19681 accttccagt tcagcacatt cagtttcggc atggggaacg ctgccgggtt gttattcgcc
    19741 ggaattatgc tgggctttat gcgtgctaac cacccgacct tcggttacat tccgcaaggg
    19801 gcattaagca tggtgaaaga gttcggcttg atggtgttta tggcaggcgt tggtctgagc
    19861 gccggtagcg gtattaataa cggtctgggc gcgattggcg gtcagatgtt gattgccgga
    19921 ttgattgtca gtcttgtgcc cgtggttatc tgtttcttgt tcggtgctta tgtattgcga
    19981 atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac gcacctgcgc gccggcaatg
    20041 gagatcatca gtgatacagc tcgcagtaac atcccggcgc tgggctatgc gggcacctat
    20101 gcaatcgcca acgtcctgct gacgctggca gggacaatca tcgtcatggt atggccagga
    20161 ttaggataaa actgaagttg ccctgcaaat gaaatttttt tgcacaaccg cagaactttt
    20221 ccgcagggca tcagtcttaa ttagtgccac tgcttttctt tgatgtcccc attttgtgga
    20281 gcccatcaac cccgccattt cggttcaagg ttggtgggtt ttttgttatc taaatcccca
    20341 aaatctccat tctctcccct gtccaaagcc ttatctgact gtcctctctt ccagaaaatc
    20401 attctttttc gtacaaagaa caaatagtta ttgagaagct tatttaaaaa cctaaacaaa
    20461 atcggtaata ttcctggctt tgcaatacac agatttttta tatggatatt aacgagctaa
    20521 aagagtgcct tcatctggaa gtcattggta aaagtaggaa attcacatgg cgtaaagtca
    20581 ttgtaagggc gatgaaacat cggcgcgttc gttacttatt ctggtggcgt atcgctaaat
    20641 acggccatga gaaaggtggt tactggcgga aaattgctgg caaaattgag cgaaaaatcc
    20701 ttgatagtta tgacgttaaa atcccattag tcgttgatat aggcaaaggg ttggatattt
    20761 catacttaac gggagttgtt atcggtcata acgtaaaaat tggtgagaat tgttccatca
    20821 aacccggcgt aaccatcggc ctacgtggcc attttgacga aatggatata cagattggca
    20881 acaatgtcac cattggttgc aacgcctcca ttctgggcgg caaagtctat attggagata
    20941 acgtcacgat aggtgcacat gcattggttt tgcatgatat cccagaaaac tccattttta
    21001 tcaataaaat agaatacgag attatcccga aaaaagttat agccgaaatg tgatcgccag
    21061 tgagatgatt agcgctattg agggaggtgt tgcgtcttat cgacctctgt agctaccctg
    21121 caaccctctc aaccatcctc aaaatctcct cgcgcgatag cggcttacgg tcggtgacaa
    21181 aatgcagcgt catcccctca ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc
    21241 attgttcgag cgtttgctga ctgcgctgca tccagttttg cattaccgtt tttaatagcg
    21301 gttttcggct agccagcgcg tagagctggt acatcagctc catgttatcc ggcgttgcaa
    21361 cctgtgagct gtagatcata tcggtgatag cctggcatgc gccttgagca tcactaacat
    21421 cgctaaaaaa tgcctgatat tgccgggaca tgatctcagt aaaactgctg aacgcctcca
    21481 gcaacaactc atcaattcct gaaaaatagt aggtcatcga tcccaacggt accccggcaa
    21541 gggtagcaat tttgcggtgc gtaacagcat gtattccgta aagtttcacc gcctccagcg
    21601 tggcctggat aattttttct cgccgttgcg gatcgttagc gcgacgcatg tcatttcctc
    21661 tgtctgcaat tgtgtacaaa tgtacacaac cttgctaatg ttgtcttccc tcttcttatt
    21721 ttgacgtgat ctatgaccgt aaattcttca cgtaatgcat tgaaacgccg aacctgggcg
    21781 ctgtttatgt tcttcttttt gccgggcctg ttaatggcgt cctgggcaac ccgtacgcct
    21841 gctatccgcg atattctctc tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg
    21901 tcgatcggtt cgatgagcgg tattctctgc tcggcgtggt tagtgaaacg ctttgggaca
    21961 cgtaatgtca tcctggtcac catgtcctgc gcattggtcg ggatgatgat attaagtctg
    22021 gcactctggc tgacatcgcc cctgctcttt gccgttggtc tcggcgtctt tggggcaagt
    22081 tttggttctg cggaagtggc gataaacgtt gaaggtgccg ccgttgagcg agaaatgaat
    22141 aaaacggttt tgccgatgat gcacggtttt tatagcctgg gcacgctggc aggcgctggt
    22201 gtcgggatgg cactgacggc ctttggcgtt ccggcaacgg tgcacatttt attggcggcg
    22261 ctggtaggca tcgcgcctat atatatcgcc attcaggcaa tccctgacgg tacgggcaaa
    22321 aatgctgccg atggcaccca gcatggcgaa aaaggcgtac ctttttatcg cgatatccag
    22381 ttgctgctga ttggtgttgt ggtgctggcg atggcctttg ccgaaggttc tgccaacgac
    22441 tggttaccct tattaatggt tgacggtcac ggttttagcc ccacttccgg ctcgctgatt
    22501 tatgccggtt ttaccctggg gatgaccgtt ggacgcttca ctggcggttg gttcatcgac
    22561 cgttacagtc gcgttgccgt ggttcgggcc agtgcgctaa tgggggcgtt gggtattggg
    22621 ctgattattt ttgtcgatag cgcctgggtc gctggggtgt ctgttgtact ctggggactg
    22681 ggtgcctcgc tgggcttccc gctgaccatt tctgccgcca gcgataccgg ccccgatgca
    22741 ccaacccgcg tcagtgtggt agcaacgacc ggttatctgg ctttcctcgt cgggccgccg
    22801 ctgctgggct atctcggcga acattatgga ttacgtagtg caatgctggt tgtactggcg
    22861 ctggttattc tcgcggctat tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag
    22921 acggcgatgg agaatagttg atgagcatta aattaattgc ggtagacatg gatggtactt
    22981 tcttaagcga tcaaaaaacc tataaccgtg agcggtttat ggctcagtat cagcaaatga
    23041 aagcacaagg cattcgcttt gtggtcgcca gcgggaatca atattatcag ttgatctctt
    23101 tcttccctga aattgctaat gaaattgcct ttgtggctga aaacggcggc tgggtagtga
    23161 gtgaaggcaa agatgttttt aatggcgagc tgtcgaagga tgcgtttact actgtcgtgg
    23221 aacatttgct gacgcgcccg gaagtggaaa ttattgcctg cggaaaaaat agcgcctata
    23281 cgctcaaaaa atatgacgat gccatgaaaa cggtggcgga aatgtattat caccgtctgg
    23341 aatacgtcga taactttgac aacttagaag atatcttctt taagtttggc ctgaatcttt
    23401 ccgatgaact gattccacaa gtacaaaaaa cattacatga ggccatcggc gatattatgg
    23461 tgccggtcca caccggcaac ggcagcatcg atctgattat ccccggcgta cataaagcca
    23521 atggccttcg ccaactgcag aaattatggg gaatagacga cagcgaagtg gtggtctttg
    23581 gcgatggcgg taacgatatt gagatgctgc gtcaggcagg ctttagtttt gcaatggaaa
    23641 atgccggcaa cgcggtcgtc gcatcggcaa aataccgggc aggctccaat aaccgtgaag
    23701 gcgtactgga tgtgatcgat aaagttctta aacacgaagc gccatttgac caataaaaac
    23761 ggcagcacgc ccggcaacac aatacctgaa tgaaggctgc ataattcgcg gaaattaacc
    23821 cgctgcatta tttcaggcga aatgattatg aaaaattgtc tactgttggg cgcgctttta
    23881 atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac
    23941 aaagtggttg tggttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg
    24001 ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt accacccgca tccttatgtt
    24061 cgccatcttg ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac
    24121 cattaattcg agaatgcctg atcgcacaaa gcaatcaggc atttttttac ccttcgtgag
    24181 aatttcccat ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaattccgt
    24241 tgacaaggtt gaagagatta aacagtccgt tgcccccgtt cagccaggca tgtttgctga
    24301 tttcaatacc gacggtaaag atcagcattt gcagcattcc catcgccgca gaaaccgtac
    24361 ctttactcat atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa
    24421 taccgaaagc ataaatactt aacccggcgg tcatccataa atacgcgtgc gatgagataa
    24481 ccgttgccgc agcagcgacc aacagaccaa tcataatcgg ccagccgccc ataataatca
    24541 gcgaacgtac ggtgcggcgc gaggtcagac gcgctaacag caagttaccc gcaattaacg
    24601 ccccgaaaat aggcacttgc agcaaaccat attcatagct gctcaactgc tcgccggtaa
    24661 tgatgataat cggcgactga gcgatccacg ccagcaacgg caggctaacg aatcccagcg
    24721 ccagcgcccc cgccacaaag cgaccgttct tcagcaccag cttatagtca cgaccgagtt
    24781 ctttcagcga cagtttctcg cctatacgcg tggcggtttc aggcatggct cgttgcagac
    24841 cgaaaaagga gatcgctgcc aatgcggcaa acaagacaaa catcccttcc cagggcagca
    24901 catggatcca cgccgcgccc accagtggac caagtagcgg agcaatcagc gccacgttcg
    24961 ccatcagcgc ggtgatcttg atacaaaccg cctcttcgaa ggattcctga attgcggcgt
    25021 atcccacagc gccaatgaaa cagaggctta tgccctgcaa gaagcgcaac agggtgaatt
    25081 gttcaatgtt ttgcgccagt aatattgcca gacaggtgac aataaaccac accacacccg
    25141 ccagcatcac cggacggcga ccaatacgat ccgacaacgg ccccaggagc cattgtaaaa
    25201 acatcccgcc cgccagatac gcagtcatcg aagtaggaac ccaatcaatg cccgcctgat
    25261 attgttccac cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa
    25321 attcgtaaag caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg
    25381 aagctaattt attttgcatg caatttcttc gccaataata atcgcgcaaa gtttaataaa
    25441 agcgcagcta acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca
    25501 ttattgcgag tatagctacg ctttctttaa gttttattta acctttgccc gttacaatca
    25561 cccaccgtaa acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct
    25621 gcactttatt ctcaacgact tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa
    25681 gttatgctgg aaaatttgaa tctctctcta ttctctctta ttaacgcgac gccagactcg
    25741 gctccgtgga tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtgccg
    25801 ctgctggccg cggtactttg gttgtggggg cttacagcac aacggcaact ggtgataaaa
    25861 atcgctatcg cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg
    25921 cacgaccgac cgtttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac
    25981 tcattcccga gcgatcacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg
    26041 catcgcctgt ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc
    26101 gtttatcttg gcgtccactg gccgctggat atgctcggtg gattgctggc aggtatgatt
    26161 ggctgcctta gtgcccagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg
    26221 caatcgtggt atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga
    26281 tctctgctaa aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg
    26341 gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagcgataag
    26401 ttacatctta aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat
    26461 ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccgcgc
    26521 agtgccagcc attacctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg
    26581 gcggcaaaac tggctgcgac gctagtagaa cccgatcaga ccctcttttt tgactgtggc
    26641 accaccacgc cgtggattat tgaagcgatt gataatgaaa tcccttttac cgccgtttgt
    26701 tattcgctaa atacctttct ggcgctgaaa gagaaacccc attgccgcgc atttctgtgc
    26761 ggtggtgaat ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgttg
    26821 aataattttt gcccggatat cgctttttat tccgcggcgg gcgtgcatgt cagtaaaggc
    26881 gctacctgtt ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa
    26941 aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt
    27001 gacctgaaac gctttgatat tgtggtgagc gattgttgcc cggaagatga gtatgtgaag
    27061 tacgcgcaga cgcagcgtat taagttgatg tattaatgac gtataaccgg atgacgtttc
    27121 gcgccatccg gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca
    27181 tcatcacgaa atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga
    27241 tcagcggacg ctgctcaatg gatttaccgt taagctggaa atcaatggtc ccgacaacct
    27301 gacctttttt cagcggtgcg gtaagctgcg gttccgttaa cgtgtagctc gccttcaggt
    27361 ttttcagctg cccacgcggt atggtcactg agcccgcttc gcctgccccg agattcactt
    27421 cgctcttatc accaaaccag acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg
    27481 tcacggtttc aaagaagcgg aaaccccagg tcaataattt ctcagactca ttaaaacgga
    27541 tacggtcggt tttcgccccc agcactacgg agattaaacg catatcgccc tgggtagccg
    27601 aagcaaccag attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca
    27661 gattgctgct ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact
    27721 ctttctcttt atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca
    27781 atgccatatc gcgcgcggta ctgaactgcc ccggcgcatc cagaccgtgc accgtctgga
    27841 aggtagtgtt ggtcagaccc agttttttgg cataaccatt catcaaacca ataaatgact
    27901 cctggctccc ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa
    27961 tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg
    28021 atgaaccacg cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat
    28081 cggtgagttt aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt
    28141 tagtcaggct cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc
    28201 tggcgtaatc cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt
    28261 cggccgcgaa tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac
    28321 gaaggagaga ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg
    28381 ttatatcgcc gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg
    28441 taagttggta aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc
    28501 atttttttgc cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag
    28561 cctgtggctg cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc
    28621 aaccaacgta aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
    28681 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
    28741 cgggctggtg ccgttgttac gtgacgacga aagtgatctc attctttggg aatcaaacgc
    28801 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
    28861 acgtcgtgcg gaagcagaaa aatggatgga ctgggcaaac cagacactca gtaatgctca
    28921 tcgcgggatc ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat
    28981 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
    29041 agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
    29101 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
    29161 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
    29221 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
    29281 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
    29341 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
    29401 aataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct
    29461 cacggcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
    29521 tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc
    29581 gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt
    29641 cagccatagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
    29701 agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc
    29761 gccctggtcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
    29821 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg
    29881 cccgccaaaa tggttgccgg tagacagttt tggcatctgg cgaaagacgg tgctaaagtc
    29941 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
    30001 atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc
    30061 taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag
    30121 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
    30181 taacataccg tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg
    30241 cagtacttcg acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag
    30301 gggcacaagg aaaaaggatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc
    30361 gtagtcagtg gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga
    30421 cgctttctcc cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg
    30481 atactcagcg ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataaca
    30541 gtcgggcaat catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca
    30601 gcatgatgaa gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta
    30661 tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg
    30721 gacatgtatc cttttgcctt cctcagttgc acgtatgcgg cgaggtcaac gcgtgcgtcg
    30781 atcaaccggt tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct
    30841 ttccagcctt gttgcaatta aatagtatgg ctattttttg agcataatgc tgtccaggat
    30901 caattcttca cgccgaatta acaaaattta catttccttc gcctccgcca gcgaatgaga
    30961 ttgcgataca aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact
    31021 gaatattttt taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct
    31081 tggctgtccg aaaaaccttg tcgattcaga gcgtattctc accgaactcc gcactgaagg
    31141 ttatgacgtg gtaccgagct atgacgatgc ggacatggtg atcgtaaaca cctgcggctt
    31201 tattgacagc gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg
    31261 caaggtaatt gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc
    31321 gaaagtgctg gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca
    31381 ctacgtgcca aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtcaa
    31441 actgacgccg cgtcattatg cctatctgaa aatttctgaa ggctgtaatc accgctgcac
    31501 cttctgcatt attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtatt
    31561 aagtgaagcg aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga
    31621 tacttccgcc tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt
    31681 aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct
    31741 gcactacgtt tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa
    31801 aatcctgccg tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat
    31861 gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg
    31921 cccggaactg accctacgct caacctttat tgtcggcttc cctggcgaga cggaagaaga
    31981 tttccagatg ctactcgact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa
    32041 atacagcccg gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt
    32101 gaaagaagaa cgctggaacc gtttcatgca gttgcagcag cagatttccg ccgagcgcct
    32161 gcaagagaaa gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc
    32221 gattggtcgc agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga
    32281 aaccaacgtt aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga
    32341 tttgtggggt agccgggttt aattaatcta aaggcagatc taacaaggat attgttcttc
    32401 gatctgcctc tactaccctt cccgatgtcc tacccttacg cacaaaatcc cctgacgcat
    32461 aaatcgagaa aaatgaatcg tactcgaggc aactctcact ttttcacacc aaattaaagt
    32521 tttgatcgca attacttgaa ataagcgtaa tggttactat agtaaacatt accacaagga
    32581 tggatgtatg ggtgtgtata aggcaaggcg attcagccaa agcacgaaaa aacttggtat
    32641 tcacgataaa gttctcatgg ctgccgctga agaagtgatg caaggcatat gggaggctga
    32701 tttaggtagc ggtgtcatca aaaaacggtt acccctccaa caaggaaaga gtggtggagc
    32761 cagaacaatt atctttttta aatcagcaaa tcatgtgttt ttttatgatg gttggtccaa
    32821 gagtggtcta agcagtaaag gaaccaaaga aatcgaagat gatgaactgg cggcctacaa
    32881 aaaaatggca aatgcatttc tggcctttag caataagcaa ataaaggatt tgatcgaaac
    32941 cggctttttg attgaggtga aaaatgagcg ataaattaca aatgatgctg gaagatgcaa
    33001 aggaactgaa tgcactgggc ctgatgtctg atagtgaggt tgatgccatt gccgtcatgg
    33061 tcaaagcccg tgaagtcagc gcccgtatag ccaaagtcaa atcaatgacg ggtgaagaga
    33121 tcaaagccat ccgcacccga tacggcatgt cacaatccgt gctggcacat acaatgggaa
    33181 tgtcaaaaga aagtgtctcg aaatgggaac gtaatgaaat aaaaccgagc ggcccggcac
    33241 tacgtatttt gaatacattg gcaataaaag ggccggaagt ttttgcaaag taaaatgttt
    33301 gcaaatgatt taccacattc attagtaccc cttgattcag gggtactaac actattctaa
    33361 ttctactgcc agctaatctg agccctgccg tttttcttac tcttatacag ccgctcatcc
    33421 gccagcattt tcaatcgttc gacggtagta cattcatttg aatgcgctac accgatactg
    33481 gtgcttaaat taatgcttcc cacctggaaa tgaacgattt cttttagaat cgatagcaac
    33541 tcttccagcg ttccacccgt ataaatcacc gcaaattcgt caccgccgaa gcggtagact
    33601 ttacctttat ctaccgggat ctgtgattta aggattttca ccactttcat caacacttca
    33661 tcgcccagca aatgaccgaa ggtgtcgtta atgtttttga atttatcgac gtcaaacaga
    33721 cacaaataac cgcctttttc cggcagcgct tttaaatctt cgtcaaagga ttttctgccc
    33781 attgctttgg tcaggtcgtc caggaacgcc gagttataca tactgtagag ttttttgaca
    33841 tcggcagccg aaaagaaaag cccgccatcc agtacgcgtt caaaatggtt attaatgatg
    33901 gcttctttct ccacaatacc acgattaatc actctgcgta ccagatagag gcaactggca
    33961 cccgatatca ctgccaccaa tgcggcaaag aaaataatca atgtacagac ccgcaaataa
    34021 acatcttttt tatacgcaaa tacgtacaat ccggtcggca aataggcaaa gtgctccatc
    34081 gcttccgaaa cggtgaagat ggcgttttta gcattatatt catcaccggt caccggttta
    34141 ccgttcttaa acagagagaa catcaggttc tcgctgtcca tcgccagcga ttttgacagt
    34201 aaagaaaaac gctccagacc aagcaaaaat atcagactac gattgtgagt taccggatag
    34261 aggtaataga cgaaggtttt accgtcaaaa gagataaacc ctgtattttt gacatgtcga
    34321 tacttcacaa cctgcataat atctgcacaa taatttttct cgaactcctt tacctgaata
    34381 cattcgtcac tggaaatcag ataaggaccg acatcgtaca aataagaggg tgaaaccgag
    34441 tcggcgtaag ctgtgcgcaa gccaaccaac agctgttcct ccgtgggatt tagcgtgttg
    34501 ataagatcaa ttgccacgct acgacgatat tcagcaatat ttgccaccgc aaaagcagta
    34561 tcaataacgg actgtttcag gctatacacc cgttccttta ccatttgagt gtagatcccg
    34621 caggcaattg ctgaaatcat gataaaaatc agcagactga ctgtaagtac gaacttattg
    34681 attctggaca tacgtaaact actcttttac taattttcca cttttatccc aggcggagaa
    34741 tggcatgggt ttctggtaca gatacccctg agcctgaaca catcctaatt tgattaattc
    34801 gcgcgcaacg tcttcgctct ctacgccttc cgcaatcacc gggatccggt tatagcgtgc
    34861 caggtcgaaa ataatgcgca caatttttcc cgcagtaccg aaaatatcgc cggtaaattg
    34921 cgagtccacc tttatgctgt ctggattaat ttcataaaaa cgtttcaggt ttgacaaacc
    34981 ggtgccaaag tcatcaatcg atatccgtac cccttgttta cgcagttgtt caatattgag
    35041 ataaaaatgt tcattaattc gctcgatcac ggtttcagtg atctcaacac agaggctgtt
    35101 cgccttcagg ccattttctt tcatgatggt ggtaaaacgc tcatgaaagt gcggttcgtt
    35161 taactgccag ggcgaaacat taatatgcag taagaaatca tccttcagcg atgcacgctg
    35221 actaaaatgt ctgaattcac ccattgccgt tttaataatc tgataaccca gctcattgat
    35281 aaacccgata tcttcagcaa tggtaatgaa cttcagcggt gaaatgatcc cccgctccgc
    35341 agacacccaa cggcacaacg cctccgcgcc cagaatggcg cgggtgttaa tatcaacgat
    35401 cggttgatag acgaggaata gttcgccaat ttccattgca tggcgaatat tacgcgcaat
    35461 ccagatattc tcttctattt cgttgagttt cgcgtcatca cagaaaataa actcggagaa
    35521 catatggtca cggatactgg aaatcgccag tttcagtttt tctgcgtaac tccaggcgtt
    35581 ttccccctgc aaaggttcac aaatggcaga cttatggata cgcagattgc ggtagccaaa
    35641 gctgctgtca aaatcgttta ccatcgctat tttacgggta aatgtctgta cattttcccg
    35701 acacagaagt aaatagagat cggcattgaa tgtcacgata tcgcagcatt gtgcatattt
    35761 ctcggcaatt cgctgacaca atttcctgac gattttattg cgttcatcaa caccaaacac
    35821 cgcttccacg gcttccagac cacacagttt gatgatcccc acagccagat actgattatt
    35881 gttggactca ataatcgcca tatttttaat aaaaccactt tcctgatcgt aagagatcag
    35941 tgagagcaat ttatttgacc gttcaatttc ggcatctttc atttccgcga tcgaattttt
    36001 gatgctgata atctctttcg catcgccttc gcgaattttc agagcctcga tctgatccgt
    36061 tgagtcaggc aaaaacgcca gcgtattaac cagctctgtc atttcagaaa attgccgacg
    36121 gaacacacga gtggtcagta aataaaccac cagcaatacg ataataatga acgcaaacgg
    36181 cagataaaaa agtttatctg atgcgttcaa taaattaccg cccggataat aaaaatagat
    36241 ttcgaataat gcgttatttg gcaccgctga tgttttttcg atatattcag tatcgacaga
    36301 atcaataata ttataattac cttcgctatt ataaataatc agtttttcac gagcgagttg
    36361 attcttggca atgatctcat ggccttgttt caaaaataga acttcatcat tagcgctgat
    36421 ttgatcaaga tactcgttca gatatcgcca gtttaaatcg acatgaattt tatccaccgc
    36481 taacaaatcg tgatctttgc cgttgatgcg tagcgtaaaa gagatacatt ttttacccgg
    36541 acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg aagccaccga
    36601 ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag
    36661 cggtccattt acttgttcct gatgataaac aacgatttta tcgttcgccc tccgcgaaaa
    36721 gagataatat ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc
    36781 cattttcagg atcacagcgc gttgaatctc ctctgtattt tttaacaaat acagtgagtc
    36841 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
    36901 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
    36961 gatgaaaaaa aggccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
    37021 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tgtctgaacg
    37081 ctgtgccgct gcaaaaacag tcccttgctg cggctattta accaaattac ccgattctac
    37141 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattact
    37201 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
    37261 accatcgccc aacaaattga acgccagtac agtcagaaaa atagccaggg ccggaaaaac
    37321 agcgacatgc ggcgcgataa ccatatccgc tcgagcctca ttgagcattg ctccccactc
    37381 tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
    37441 ggtaccaatg cgcatggtga aaaacaccac gatagaagag acggtcccag gcaggatatg
    37501 acgcaacaaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
    37561 ctgtttcaac accagcgtgt tgccgcgcac caggcgggca aacgcaggga tggaaaaaat
    37621 ggcgactgca ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
    37681 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca
    37741 ccagccttca taatatccag ccagcaagcc cagcaacgtc cctatcgccg caccgataaa
    37801 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggactc ggctgaaaat
    37861 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
    37921 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
    37981 cacaatcaat aaaataacga acaatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
    38041 tcgccagaat tcatgccacg gtgtacgtac ctggtcaggt ttgaccagtg gcatggcgtt
    38101 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
    38161 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
    38221 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
    38281 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tggaaccacc gagcaaaaag
    38341 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttgcgtag cccgtgtttg
    38401 aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
    38461 aaaacgtcga caaacgacgc gcgggtaaag cgcgccatca cggcggccac tgccgcgccg
    38521 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
    38581 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
    38641 atccccgaca ccgcaatggt catactcaat cgatccggcc aacggttacg ccagacggcg
    38701 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
    38761 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
    38821 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
    38881 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgggc
    38941 ccggcaatca atcgcgccgg atcgccgggc agcatatgga caaataaaaa caccagcacc
    39001 gagacgataa acagcgtcgg aatcaacccc agtaatcgtt tgataacgta attaagcatt
    39061 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
    39121 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
    39181 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
    39241 ccgccggatc attagttttc agtgcctgag ccaggaagtc atccacctgt ttattgctgt
    39301 aaaacgcggt attaaacagc gtcggtggcc agttttgcga ggcaaacaac ggcgatagtg
    39361 cccagtcggc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
    39421 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcagtcacct
    39481 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
    39541 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
    39601 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
    39661 cgatactcgg cggtaccaca ccagtagctg gcgttgcata gcccgcgaag gccactttca
    39721 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaatg
    39781 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
    39841 cgatattttt gtttttctcc agcagtgcgg cctgctcgta aggaatgggg aaagcaaact
    39901 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
    39961 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
    40021 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
    40081 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
    40141 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
    40201 cgatcgcttc cgttttagca atattcttat acaggttata gcgtttaaga tgattcgccg
    40261 gatcgctggc ccggtccaga ttcgctttca ccgccgcggc gttgaaatcg gtgccatcct
    40321 ggaatttaat tccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat
    40381 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
    40441 aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga tcgagcgtgg
    40501 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
    40561 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
    40621 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
    40681 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
    40741 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
    40801 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
    40861 cgtcgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
    40921 cgctctacca cagccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
    40981 agatcgagca acaagttgat aatctgcccg cgaattgaaa catccagcgc cgaaacggct
    41041 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
    41101 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
    41161 cgctccagca accacgcaac gcgtgcagcc gcttctttac ctggcaataa accgtgtacg
    41221 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
    41281 tcctgaaaaa taaactgaat atcgcggcgt aatgcctgaa gtttgccggg tgacaaggta
    41341 tcgattcgct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
    41401 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
    41461 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
    41521 aaaccgctgc gcaaagggaa acgggtgacc agattacgca cccgtaaaac aggttcgcca
    41581 tccaccaccg ttttctgctc gatggggggt tcctgtttcg ctggatgttc aagcgatatc
    41641 aacgggaaac gtcggggata atctaaccct ttcatcgcac caagttgcgg aacagcagct
    41701 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacctgtt
    41761 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
    41821 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
    41881 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggctatcagc
    41941 actgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
    42001 agttgatgcg gataacgtga aagaatggtt tgtgcctccg gaatgcgtac ctgatccagc
    42061 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
    42121 tcggcaatct gttcacccac agtaaatact gggttcagcg atgtcatcgg ctcctgaaat
    42181 atcatcgcca tatccgcacc gcgcacatgg cgcatttgtg cagcgctctg ctcgctaagt
    42241 tcaatcacat cacgactgcg ccgccgcaac agcattttat cgcactgcac taaaccgccc
    42301 gcctgttcca acaagcgcat caatgccagc gcagtcactg acttaccgga gccggattcg
    42361 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
    42421 attttctgct ggtcctgcat aaaggcaata ttaagatttt caaccgccag cacattaccg
    42481 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
    42541 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
    42601 aacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
    42661 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
    42721 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccgg tacaagaaac
    42781 cgccacactg gcgttattgg cgtagcatcc ggcacccact aaggggctat cgccaactcg
    42841 tccgggtaat ttattggtca ttccgcccgt ggacgtggct gccgccagat tgccgtctaa
    42901 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
    42961 atggtcgagg actgttgccc cttcctcgcg cgctgccatt agttgttcat aacgcaaagg
    43021 cgtggagaaa atctccggtg agacgcgctc catgccatga gcaaacgcaa aattttctgc
    43081 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
    43141 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
    43201 cacacaggcg tccagttcat gcgtttcatc acgcgtaaag acggcgccaa ttccggcgtt
    43261 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgaactttc
    43321 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct cgatgtagcg
    43381 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
    43441 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
    43501 tatcagaatt attatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
    43561 gtaaaggcag gacccagcgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
    43621 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
    43681 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
    43741 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
    43801 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
    43861 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccatacc
    43921 atggtgaatg gcctgcgggt acctgcattc gtattatgac cggtgcgccg gtgccggaag
    43981 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
    44041 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
    44101 cggttgtttt cccggcgggg actcgcctga ctaccgcaga gctgccagta attgcttcac
    44161 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
    44221 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgat accaaccgtc
    44281 tcgccgtaca cctgatgtta gaacagttgg gatgcgaggt aatcaactta gggattatcc
    44341 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtgg
    44401 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
    44461 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
    44521 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
    44581 ccttctatca actggtacag cctttgctgg caaaactaag cggtaacacc gccagcggcc
    44641 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggtcgccttg
    44701 atttccagcg cggcgtgctg caacgcaacg ccgatggcga actggaagtg acgaccactg
    44761 gacatcaggg ttcacatata ttcagctcct ttagcctcgg caactgcttt atcgtactgg
    44821 aacgcgatcg cggcaatgtg gaagtgggcg aatgggtgga agtagaaccg tttaacgcgt
    44881 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
    44941 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
    45001 ggtagtcggc ctcggtggac tcggctgcgc agcttcgcag tatctggcaa gcgccggtgt
    45061 cggcaacctg acgctgctcg acttcgactc ggtttcgctc tcgaatctgc aacgccagac
    45121 gctgcacagt gatgctacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac
    45181 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctggatg acgcagaact
    45241 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
    45301 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
    45361 tcgtatggaa ggtcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
    45421 ccttagccgt ttgtttggcg agaacgcgtt aacctgcgta gaagcaggcg tgatggcacc
    45481 attgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaagctgc tggcaggtta
    45541 tggcaaaccg gccagcggca aaatcgtgat gtacgatgcg atgacctgtc agtttcgcga
    45601 aatgaaactg atgcgcaatc cggggtgtga agtgtgcggg cagtaattgc cacctccagg
    45661 cctgataaga cgcggcactc cgccacgtca tcagcaattt atgaggatgc agaacgtccg
    45721 gttaaatcga cgttctgcca aacgctccct gccagtcctg ctcaaacttc gccacagcgg
    45781 catcaaccgc cggatagcta atcatctgtt gtgccacatc cagtggcaga gtaattgatt
    45841 cacatcctgc cagtaagcag tccagcgcct gacgcggggt tttgaaactt gctgccagca
    45901 ctttcgcctg cggcgcatgc attttcaata actggtgtaa gtcggtcaca gtctgaatgc
    45961 cgctaccgcc ctgagcatca atacgattaa cgtaaggcgc aacatattcc gcacctgcca
    46021 gcgccgacag caacccttgt gccgcgccat ataccgcggt tcccagcgtc ggaatccctt
    46081 ccgcttttaa catcttaatc gctgccagcc cctcggcggt caccggaact ttcaccacga
    46141 tatccgcaat aatagaacgc aatttacgtg cgtcattcac catcccttca gcagtgatag
    46201 ccattacctg ggcaaacaga cgcccctgac cgcccatcgc ttcatgaagt tccggaagca
    46261 caacctccag cggtttttta cctgcggcga taatgcttgg gttagtggtc acgcccgcca
    46321 gcggaaaaat acgtgacagc gccttcaccg caacaacgtc tgaagtatcc agatacagtt
    46381 ccataaccat cctcaaaatg tactttttgt ctgaatgtag actatcacgg gaaaactctc
    46441 tgaacagttg acgcacatca atctaacttt cattcgaaag tattttaatg tttgaacgaa
    46501 acagagaggc aactatgatt ttcaatattc agcgctactc gacccatgat ggccccggta
    46561 tccgcacggt cgtatttctt aaaggctgtt cgctgggctg ccgctggtgt cagaacccgg
    46621 aaagccgcgc ccgcacgcag gatctgttgt atgacgcacg actgtgtctg gaaggctgcg
    46681 agctgtgcgc taaggccgcg ccggacgtga ttgagcgcgc gttgaatggt ttgcttattc
    46741 atcgggaaaa gttaaccccg gagcatctga cggcgctaac cgactgctgc ccgacacagg
    46801 cgttaaccgt gtgtggtgaa gtgaaaagcg ttgaggagat catggcgacc gttttgcgcg
    46861 ataaaccgtt ttacgatcgc agcggcggcg gcttaacact ttctggcggt gaacccttta
    46921 tgcagccgga aatggcgacg gcgctactgc aagccagcca cgaggccggc attcatactg
    46981 cggtagaaac ctgtctgcat gtgccgtgga aatatatcgc cccttctctg ccctatatcg
    47041 atctgtttct tgccgattta aaacacgttg ccgacgcgcc gtttaaacag tggaccgacg
    47101 gtaacgccgc cagggtgctg gataacctga aaaaactcgc cgcagcgggc aaaaaaatca
    47161 ttatccgcgt gccactgatt cagggcttta atgccgacga aacctctgta aaagccatta
    47221 ccgattttgc cgccgacgag ctgcacgttg acgaaattca tttcctgccc taccacacgc
    47281 tgggcatcaa caaatatcac ttacttaatc tgccctatga cgccccggaa aaaccgcttg
    47341 atgcgccaga actgctcgac tttgcccagc agtatgcctg ccagaaaggg ttaaccgcga
    47401 ccttacgagg ataacaacca tgaccacact gaaactggac acgctcagcg accgcattaa
    47461 agcgcacaaa aatgcgctgg tgcatattgt gaaaccgccg gtttgtaccg agcgcgcgca
    47521 gcactatacc gagatgtatc aacaacatct cgataagccg atccctgtac gtcgcgcgct
    47581 ggcgctggca catcacctgg cgaatcgcac tatctggatc aagcacgatg agttgatcat
    47641 cggcaaccag gcaagcgaag ttcgcgccgc gccgatcttc ccggaatata ctgtctcgtg
    47701 gatcgaaaaa gagattgatg atctggcaga tcgtcccggt gctggctttg cggtgagcga
    47761 agagaacaaa cgcgttctgc atgaagtgtg cccgtggtgg cgcggtcaga ccgtacagga
    47821 tcgctgctac ggcatgttta ccgatgagca aaaaggtctg ctggcgaccg gcatcattaa
    47881 agcggaaggc aatatgacct ccggcgatgc gcacctggcg gtcaatttcc cgctgctgct
    47941 ggaaaaaggg cttgatggtc tgcgcgagaa agtagcggaa cgtcgctcgc gcatcaacct
    48001 gacggtgctg gaagatttac acggtgagca attcctgaaa gcgattgata tcgtgctggt
    48061 ggcagtcagt gaacatattg aacgtttcgc tgccctggcg cgtgaaatgg ccgcgaccga
    48121 aacccgcgaa agccgtcgcg atgaactgct ggcgatagca gaaaactgcg atctcatcgc
    48181 ccaccagccg ccgcagactt tctggcaggc gctgcaactg tgttacttca tccagttgat
    48241 attgcagatc gaatctaacg gtcactcggt atcgtttggt cgtatggacc agtatctcta
    48301 tccgtactat cgtcgtgatg ttgaactgaa acagacgctg gatcgcgaac acgccatcga
    48361 attgctgcat agctgctggt tgaaattgct ggaagtgaac aaaatccgct ccggctcgca
    48421 ctcgaaagcc tctgcgggaa gtcctctgta tcagaacgtc accattggcg gacaaaatct
    48481 ggttgatggt caaccaatgg acgcggtgaa tcccctctct tacgcgatcc tcgaatcctg
    48541 cggtcgcctg cgttccactc agcctaacct cagcgtgcgt taccacgcgg gaatgagcaa
    48601 cgatttcctc gacgcctgcg tacaggtgat ccgctgcggc ttcgggatgc ccgccttcaa
    48661 caacgacgaa atcgtcatcc cggaatttat taaactcggt attgaaccgc aggacgctta
    48721 cgactacgca gcgattggtt gtatcgaaac cgccgtcggt ggcaaatggg gctatcgctg
    48781 taccggtatg agctttatca acttcgcccg cgtgatgctg gcggcgctgg aaggtggtcg
    48841 ggatgccacc agcggcaaag tgttcctgcc acaagaaaaa gcgttgtcag cgggtaactt
    48901 taacaatttc gatgaagtaa tggacgcgtg ggatacgcaa atccgttact acacgcgtaa
    48961 atcaatcgaa atcgaatatg tcgtcgacac catgctggaa gagaacgtgc acgatattct
    49021 ctgctcggcg ctggtggatg actgtattga gcgagcgaaa agcatcaagc aaggcggcgc
    49081 gaaatatgac tgggtttctg gcctgcaggt tggcattgcc aacctcggca acagcctggc
    49141 ggccgtgaag aaactggtat ttgaacaggg cgcgattggt cagcaacagc tggctgccgc
    49201 actggcggat gacttcgacg gcctgactca tgagcaactg cgtcagcggc tgattaacgg
    49261 tgcgccgaag tacggcaacg acgatgatac tgtcgatacg ctgctggctc gcgcttatca
    49321 gacctatatc gacgaactga aacagtacca taacccgcgc tacggtcgtg gtccggttgg
    49381 cggcaactat tacgcgggta cgtcgtctat ctccgctaac gtaccgtttg gcgcgcagac
    49441 tatggcaaca ccggacgggc gtaaagccca caccccgctg gcagaaggcg caagcccggc
    49501 ttccggtact gatcatcttg gccctactgc ggtcattggc tcggtgggta aactgcctac
    49561 ggcagcgatt ctcggcggcg tgttgctcaa ccagaaactg aatccagcca cactggagaa
    49621 cgaatctgac aagcagaaac tgatgatcct gctgcgcacc ttcttcgagg tgcataaagg
    49681 ctggcatatt cagtacaaca tcgtttcccg cgaaacgctg ctggatgcga aaaaacatcc
    49741 cgatcagtat cgcgatctgg tagtgcgtgt cgcgggttat tccgccttct tcaccgcgct
    49801 ctctccagac gctcaggacg atatcatcgc ccgtaccgaa catatgctgt aatcccctca
    49861 gcccggcgga gacgtcgccg ggctaaatca ttcacaccat caatttcact ctcactttca
    49921 ttcgaaatat aatttgtgct ctgcgtcaca ttgttattac actgttacgg tttcattttc
    49981 gagccaggag cacacctatg agcgtaaaag ttatcgtcac agacatggac ggtacttttc
    50041 ttaacgacgc caaaacgtac aaccaaccac gttttatggc gcaatatcag gaactgaaaa
    50101 agcgcggcat taagttcgtt gtcgccagcg gtaatcagta ttaccagctt atttcgttct
    50161 ttcctgagct aaaggatgag atctcttttg tcgcggaaaa cggcgcactg gtttacgaac
    50221 atggcaagca actgttccac ggcgaactga cccgacatga atcgcggatt gttattggcg
    50281 agttgctaaa agataagcaa ctcaattttg tcgcctgcgg tctgcaaagt gcatatgtca
    50341 gcgaaaatgc ccccgaagca ttcgtcgcac tgatggcaaa acactaccat cgcctgaaac
    50401 ctgtaaaaga ttatcaggag attgacgacg tactgttcaa gttttcgctc aacctgccgg
    50461 atgaacaaat cccgttagtg atcgacaaac tgcacatagc gctggatggc attatgaaac
    50521 ccgtcaccag tggttttggc tttatcgacc tgattattcc cggtctacat aaagcaaacg
    50581 gtatttcgcg gttactgaaa cgctgggatc tgtcaccgca aaatgtggta gcgattggcg
    50641 acagcggtaa cgatgcggag atgctgaaaa tggcgcgtta ttcctttgcg atgggcaatg
    50701 ctgcggaaaa cattaaacaa atcgcccgtt acgctaccga tgataataat catgaaggcg
    50761 cgctgaatgt gattcaggct gtactggata acacatcccc ttttaacagc tgacctctct
    50821 accggagcca tgcgctccgg ttttcccttc tttttacatc ctgtacatca catcaaatca
    50881 ttaaacttgc attaacttat ttataactta tattgtcaca tgcaaatcag catagtttcg
    50941 aatgaaagtt aatgcgaggt ggttatggct tctactttta ccagcgacac attgcctgcc
    51001 gatcacaaag cagctatccg tcagatgaag cacgcgctgc gggcgcagct tggcgacgtc
    51061 cagcagatct ttaatcagct aagcgatgac attgccacgc gagtggctga aatcaacgca
    51121 ctcaaagcac agggcgatgc cgtctggccg gtgctgtctt atgccgatat caaagcaggt
    51181 catgttactg cagagcagcg cgaacagatt aaacgtcgcg gttgtgcggt gataaaaggc
    51241 catttccccc gcgaacaagc gctaggctgg gatcagtcga tgctggacta tctggaccgc
    51301 aaccgctttg acgaggtcta caaaggcccc ggcgataatt tcttcgggac gctcagcgct
    51361 tcacgtcccg agatttaccc catctactgg tcgcaggcgc aaatgcaggc tcgccagagt
    51421 gaagaaatgg cgaatgcgca gtcgtttctc aatcgtctgt ggacatttga aagtgatgga
    51481 aagcaatggt ttaacccgga tgtgagcgtc atctaccctg accgtatccg ccgccgtccg
    51541 cccggaacga cctccaaagg tcttggagcg cataccgact ccggggcact ggaacgctgg
    51601 ctgcttccag cgtatcagcg cgttttcgcc aacgtcttta atggcaatct ggcgcaatat
    51661 gatccctggc atgcggcaca tcgtacggaa gttgaagagt acacggtgga caacaccacc
    51721 aaatgttccg tgtttcggac attccagggc tggacagcgc tctctgatat gctgcctggt
    51781 caggggctgc tgcacgtcgt gcccattcct gaagccatgg cgtacgtact gttacgtccg
    51841 ctgcttgatg atgtgccgga ggatgaactg tgcggcgtag cgcccggaag agtattgccg
    51901 gtatcagagc aatggcatcc actgttgatt gaggcattaa ccagcattcc acaactcgaa
    51961 gccggagact ccgtctggtg gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat
    52021 caacagggct ggggcaacgt gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt
    52081 gcctacgcgc acaaggtgaa ggccgcactg gaaaaaggcg catcgccggg cgatttcccg
    52141 cgcgaggact atgaaacaaa ctgggaagga cgctttacgc ttgccgacct caacattcac
    52201 ggtaagcgag cgttgggcat ggatgtttga gagttaatgg aagtgaattg atgtgggatt
    52261 aagaaaatcg attcgacgtt actcagattg ctgacaagtg cgctttctta atgcctgatg
    52321 cggcgttaac gccttatctg gcctacaaag tcctgcagat tcaatacatt acaggtactg
    52381 cgtaggcctg ataagcatag cgcatcaggc aatcttgcgt ttgtcatcag ccgccgtcac
    52441 cccgacagta tcggggtgac ggataataac ttactcgatc cctttactac gcaggtaatc
    52501 ttcgtaatta ccgctaaagt cgatcacgcg ttccggggtg atttccagaa tgcgggtcgc
    52561 cagggagctt acgaactcac ggtcgtgtga aacaaagatc agcgtgccct gatacagttc
    52621 cagcgccatg ttcagtgact cgatggattc catatccagg tggttggtcg gttcgtccat
    52681 gatcagaatg ttcggcttct gcatcattaa cttaccaaaa agcatccgcc ctttttctcc
    52741 accggaaagc actttagcag gctttttgat gtcgtcctgg ctgaacagca aacgaccgag
    52801 aatactgcgc accgcctgct cgtcatcgcc ttcctgcttc cactggctca tccattcgaa
    52861 cacggtcaga tcattttcaa actcatattc gtggtcctga gcatagtaac caatgcgcgc
    52921 gttttcagac cattttacgg tgccgctgtc cggttgcaga tcgcccacca gcgttttcaa
    52981 cagcgttgat ttaccgacgc cgttggtacc cagtaccgcc agtttttcac cgacttccag
    53041 cagcaggttg agatttttaa acagcggacc gttatcaaac cctttggtca gaccttccac
    53101 ttccagcgcg ttacggaaca gcttcttatc ctgttcaaaa cggatgaacg ggttctgacg
    53161 gctggaggct ttaacctctt ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc
    53221 ctggcgagat ttcgaggcgt tggcgctaaa gcggctaacg aaagattgca gctcagcaat
    53281 ctgcgctttc ttcttggcgt tatcagccag cagacgttca cgcgcctggg tcgccgccgt
    53341 catgtactca tcgtagttgc ccggataaac gcgcagctcg ccgtaatcca gatccgccat
    53401 gtgggtacag accatgttaa ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc
    53461 acgctcgttc agcacctgtt ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg
    53521 ttcgtcgagc aggagaatgt ccggatcagc aaacagcgcc tgcgccagca acacacgcag
    53581 cttccagcca ggagcaactt cactcatcgg gccgtagtgc tgttccactg gaattcccac
    53641 gccaagcaac aattcaccgg cacgagcttc cgcagagtaa ccgtccattt cgccgtattt
    53701 aacttccaga tcggccactt tatagccgtc ttcttcactc atttccggca aagcatagat
    53761 gcggtcgcgc tcctgcttca cttcccacaa ctctttatgc cccatgatca ccgtatccag
    53821 cacagtgaac tcttcaaagg caaactgatc ctgacgcagc ttaccaatgc gctcgttggg
    53881 atcgagggaa acgttaccca acgtcggctc taagtcgccg ccgaggatct tcataaaggt
    53941 ggatttacca ctaccgttcg cgccaatcag gccgtaacgg ttgccgccgc caaatttgac
    54001 ggaaatgttt tcaaacaacg gcttactgcc gaactgcatg gtgacgttac tggaaactaa
    54061 cacaggcgta tcctgaaaag agatatgaca aaccgcgcat tatgccataa gtctgaaaga
    54121 agatcctgct cctcgcttaa ggtctacact caagccaaag gcaaaaaatg tgatttcgta
    54181 cacatctgat ttcactgtga gctggaatga acttataatg cgcttccaat actctaatat
    54241 tctcaaccca atggcctgcc aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa
    54301 cattattcgc agcggccttc gctgttgtcg gcttttgcag taccgcctct gcggtaactt
    54361 atcctctgcc aaccgacggg agtcgcctgg ttggtcagaa tcaggtgatc accattcctg
    54421 aaggtaacac tcagccgctg gagtattttg ccgcggagta ccagatgggg ctttccaata
    54481 tgatggaagc gaacccgggt gtggatacct tcctgccgaa aggcggtact gtactgaaca
    54541 ttccgcagca gctgatcctg ccggatactg ttcatgaagg catcgtcatt aacagtgcag
    54601 agatgcgtct gtattactat ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca
    54661 ttggtcagtt aggcaaagat acgcctatca actggaccac caaagttgag cgtaagaaag
    54721 caggcccgac ctggacgccg accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac
    54781 cgcttccggc tgtcgttccg gcaggtccgg ataacccgat ggggctgtat gcactctaca
    54841 tcggtcgcct gtatgctatc catggcacca acgccaactt cggtatcggt ctgcgtgtaa
    54901 gtcatggttg tgtgcgtctg cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg
    54961 tcggtacccg cgtacagttt attgatgagc cggtaaaagc gaccaccgag ccagacggca
    55021 gccgttatat tgaagtccat aatccgctgt ctaccaccga agcccagttt gaaggtcagg
    55081 aaattgtgcc aattaccctg acgaagagcg tgcagacagt gaccggtcag ccagatgttg
    55141 accaggttgt tcttgatgaa gcgatcaaaa atcgttccgg gatgccggtt cgtctgaatt
    55201 aatcgtcaaa acgtaaaaca aaggcggact cataatccgc ctttttttat ttgccagacc
    55261 ttaccaggcc gggatcataa ccaacaaaac atatcccacc agcgccgccc acaacagcat
    55321 cggtattgaa tagagatgga aacgccacca gatgcggcga tcgttcgcca tacgtagcgc
    55381 aatcaaattt gccagcgatc cgggtaataa cccaaagcca cctacgttta ccgcccatgc
    55441 cagtaataaa gacggcggca catagttcag caacaatatg gtactcggca cgttactgat
    55501 cacctgcgat aaaccgattg ccgttaacca taacccgggt tcagataaat gactcacgtt
    55561 acccaacacg ccttgcaacg ctggaagctg gatcagtaaa tggacgtcga taaacatcgc
    55621 cataaacacc agcagcaacg tccagtccac actcaacacc acgcgacgcg ccagcagcgc
    55681 aaagcccgcc gccacaatca ccagtcccca cagctcttgt ttgaactcca gcgccgtcag
    55741 aaagacgata tacagcccca gacaactcca caccagccgc ggtttccact ccggtgtttg
    55801 cacccccgta tggtattgga gtgcctttcc agggaaacaa caccagcaca acagcaggag
    55861 tgtcagcatc attgcgccag ccagcggtgc catttgggca ataaatccgg caaacgaaag
    55921 accagaacgt ccccagataa gaatattttg cgggttgcca attggcgtca gtagcgaacc
    55981 agcgttgact gccagcgcct caaaaataat cagccgatta accgggatct cacacagcct
    56041 ttttagcgtg atagtcagcg gaacaacaat aaacagcgcg acatcgttgg tcagaaaggt
    56101 agaaagcagc gccgccgcca gcaccataaa catcgccagc cgacgctccg tagcaaagcg
    56161 gcgcaccatt ttgcgcccca gcacatcaaa ataaccgctt aactccacac ctttggtcag
    56221 cagcatcagg ccgcttaagg tgatgatggt gtgccagtcg atagcagcag gccaggattt
    56281 cggtgcaaag ggaacgaaaa aacttaatcc gataccaaca agaattaata actgaaaaaa
    56341 acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg tttctgggta
    56401 aacaaacgaa aggcatccag cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg
    56461 cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac tatctgatgt
    56521 cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac gccacgccag
    56581 gggatcattt caatcagccc catagttgcc agtcgcttaa gcattttagc caccgtcggt
    56641 tgcgaaactc ccaaacgagc agccatgtct acctggcgag cttcccccac ttccctgatc
    56701 aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc ctctctgacc
    56761 tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt ttttgctgtt
    56821 ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata catagcgttc
    56881 tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta acgttttagc
    56941 aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc cctcaaggat
    57001 cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt
    57061 tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg atatggtcaa
    57121 caacaaaccg cagcaagata aaccttccga taaatagcgg cgtcgcggta cgccgcttcc
    57181 ctcctgcttt catgcaggca taacgcgttt tggtctgaaa aaccccactt tttgtcggat
    57241 ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg cggctcgctc
    57301 tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt tcccctcttc
    57361 acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt ttgttacgat
    57421 agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc
    57481 cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta ggttacccct
    57541 tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg cgaacattgc
    57601 ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt tatttccctt
    57661 ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac tccaccaata
    57721 tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg gtcggcttat
    57781 ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct
    57841 ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttt gttgctcccg
    57901 tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat ccggtatttc
    57961 gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa gaacggatgg
    58021 caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg aagtatcaga
    58081 cctatgtggt ggtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc
    58141 actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct gactacattg
    58201 ccgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgtt gtcgatggca
    58261 agccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt
    58321 ggttttccaa ccagggtcaa atcggcgaat acgatacagc catcgccagc atcgccaaac
    58381 gagcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa aacaccaaag
    58441 acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg caaccgcagc
    58501 tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca caaggaaaat
    58561 acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg acgcaaacag
    58621 acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc ttctcgctgg
    58681 tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg caataccttg
    58741 cccatgatga taagtatcag caaaatttcc aggtgccttt tatggtcatt tccagcgacg
    58801 ataaagcgca tcgtgtgatt aaagcccgcc gctcagccaa tgacttctta ggctttttct
    58861 cccagtggac gggaattaaa gcgaaggaaa ttaacatcaa atacccgttt atatctgaga
    58921 agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat tacaaccatc
    58981 tcggaaccga tattttcgac ccgaaacctt aaaacaaaaa tccgccccga gaggcggatt
    59041 ttttatatca ccgaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt
    59101 caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt
    59161 tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg
    59221 ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag atgcttgccc
    59281 agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt
    59341 caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg
    59401 ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc
    59461 cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac
    59521 ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca
    59581 cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta atgggaaact
    59641 cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc
    59701 tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc
    59761 ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca
    59821 ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc
    59881 agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat
    59941 tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg gcgtctattc
    60001 agggtggagc ttcgttagct aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg
    60061 cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc
    60121 gctttgccaa agagcaacga ttaccgctgt tgttttacgg cgtttcgctg ggggggatga
    60181 attatctttt ttatctttct attcagacgg taccgctggg tattgcggtg gcgctggagt
    60241 tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg
    60301 ttgtgctggc ggttcttggt ctgtggttcc tgctacctct ggggcaagac gtttcccatg
    60361 tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt
    60421 taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtggcaatt ggttcgttga
    60481 ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact
    60541 ggtcggttat tccattgggt ctggcggtcg ctattctctc gaccgctctg ccttattcgc
    60601 tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg
    60661 aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac
    60721 agctgctggc gctcggcgca attatcgccg cttcaatggg ttctacgctg acagtacgca
    60781 aagagagcaa aataaaagaa ttagacatta attaaattta catttctgca tggttatgca
    60841 taaccatgca gaatttctcg ctacttttcc tctacaccgt ctttatatat cgaattatgc
    60901 aaaagcatat ttattccgaa aattcctggc gagcaaataa ataagaattg ttcttatcaa
    60961 tatatctaac tcattgaatc tttattagtt ttgtttttca cgcttgttac cactattagt
    61021 gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat
    61081 tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca
    61141 aaagcgacca atctgcttta tacccgcaac gatgtctctg acagcgagaa aaaagcaaca
    61201 gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa
    61261 gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc
    61321 cgcactgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca gctaggcggt
    61381 gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg
    61441 gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct
    61501 aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc
    61561 gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc
    61621 catcgctgat ggtgcagaac tttagtacac ggtaaaagcg gcttcctgac aggaggccgt
    61681 tttgttttgc agcccgcctc aacgcactta tttagtgcat ccatctgcta tccccagctg
    61741 attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga ttgttttttc
    61801 atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc accagtttgg
    61861 tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca cgtttttgcc
    61921 gcatctcgaa aaatcaatga gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc
    61981 acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt
    62041 ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt
    62101 agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt
    62161 gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact
    62221 gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc
    62281 gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta
    62341 ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa
    62401 agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg attacgcgaa
    62461 agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga
    62521 actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat
    62581 caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata
    62641 cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa
    62701 aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttcg gtactgaacc
    62761 gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctcccct ctggggagag
    62821 gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt
    62881 tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat
    62941 tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg
    63001 cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa
    63061 ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg tccaggtgat
    63121 gtttatctat tttgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc
    63181 ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc
    63241 ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg
    63301 ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg
    63361 taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg gcgtggcgga
    63421 actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag
    63481 cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc tgcgtcgtct
    63541 ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca
    63601 acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc
    63661 gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc
    63721 acctccggcg atctgattgt cgatggcctg aaggttaacg atccgaaagt tgacgagcgc
    63781 ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg
    63841 gcgctggaaa acgtcatgtt tggcccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg
    63901 gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac
    63961 ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg
    64021 aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat
    64081 gaagtgctga aggttatgca agatctggct gaagaaggga tgacgatggt gatcgtgacc
    64141 cacgaaatcg gttttgccga gaaagtagct tcgcgcctga tctttatcga caaaggccgg
    64201 attgcggaag atggcgatcc acaagtgttg atcaaaaatc cgccgagcca gcgcttgcag
    64261 gaatttttac agcacgtctc ttaataagac acattgcctg atgcgctacg cttatcaggc
    64321 ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga taaggcgttc
    64381 acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata tcccttcctc
    64441 cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc ccttccccag
    64501 catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg tggatcctgt
    64561 tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc ggcgttacaa
    64621 ccacaacgac aacggactca acgactgaac cggccccgga accggatatc gaacaaaaaa
    64681 aagcggccta tggcgcactg gcggatgtgc tggataatga cacctcgcgt aaagagttga
    64741 tcgaccagtt gcgcaccgtc gccgcgacgc cacctgctga accggtcccg aagatcgtgc
    64801 cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc agccgccatt
    64861 atggtgaagc cctttccgcc cgcttcgggc agctttatcg caatatcacc ggctccccgc
    64921 ataagccgtt taatccacaa accttcagca atgcgctgac ccatttttca atgttagcgg
    64981 ttttagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgctg tatcgcaaaa
    65041 tgggtcagtg ggcgcggcaa aaaaatcgtg agcgcagtaa ctggctgcag cttccggcga
    65101 tgattatcgg ggcgtttatt atcgacctgc tgttactggc actgacatta tttgtcggcc
    65161 aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa caaagtttgt
    65221 ttctcaacgc ctttgccctg attgaatttt tcaaagctgt actacgcctg attttttgcc
    65281 caaatgtggc ggagctgcgc ccgttcacga tacaggacga gagcgcccgt tactggagtc
    65341 gtcgcctgag ctggttaagc agtttgatag gttatggcct gattgtggcc gtgccgatta
    65401 tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt atgctgtgca
    65461 tgaccgtctg ggcgttgtac ctgatctttc gtaataaaaa agagattacc cagcatttgc
    65521 tcaacttcgc ggagcattcg ctggcctttt tcagcctgtt tatccgcgcc tttgcgctgg
    65581 tgtggcactg gctggcaagc gcctatttta tcgtgctgtt tttcttttcg ttgttcgatc
    65641 cgggcaacag cctgaaattt atgatgggtg caacagtgcg cagcctggcg attattggta
    65701 tcgcagcatt tgtttccggt atgttttccc gctggctggc aaaaaccatc actctctcgc
    65761 cacatactca gcgtaactat ccggagttgc aaaaacggct gaatggctgg ctttcggcgg
    65821 cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggctgt aatgctgctg ttgagcgcat
    65881 ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa accgtagata
    65941 tcctgatccg tatcgcactc attcttttct tctcggcggt tggctggacg gtgctcgcca
    66001 gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc agcgcccgca
    66061 cgcgtaccct gcttacgctg tttcgtaacg cgctggcggt gattatcagt accatcacca
    66121 tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca ggtgccgggg
    66181 cattaggtct ggctatctcg tttggttcgc aaacgctggt gaaagatatt atcaccgggg
    66241 tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc gggccgttga
    66301 ccggcactgt ggaacggatg tcgattcgct ccgtgggcgt acgccaggat accggggcgt
    66361 accacatcat tccgtggtct tcgattacca cctttgccaa ctttgtccgc ggcattggtt
    66421 cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc aatcaggcac
    66481 tgaaagatgc ggtagcggaa ttaatggaaa acgaagaaat tcgcgggctg attattggtg
    66541 aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg cgtgtttcgt
    66601 tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc caggtgaaaa
    66661 aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg ctgcctgctc
    66721 cgggcgcgac cccggctgaa ccgttaccgc cgggggagcc aacgctttaa cgctggcgat
    66781 tgacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag cgactctgct
    66841 tttgcccctg ggccatctct tttttaacca ccttcaccgc gcccacgtcc gtcagggcac
    66901 gatacaacgg cggtaagttt tcaccacgag ataccagtga agtaaaccac atcacctgct
    66961 tcgcgaagcc tttgctctct tcaatcatct ttttgataaa ggtgacttca ccgccttcac
    67021 accacaactc ctgttgctgg ccaccaaagt tcagtgcatc gtctttgttc agccccaggt
    67081 tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg aatggcgggt
    67141 tacacaaggt cgcgtcgtat tgctcgtttt tatggatgat tccgttaaaa atcgccccgc
    67201 tctctttttg ccgacgcagg cgaatggcgc gattaagccc cggattagca ctgatgatcg
    67261 cctgcgcgct gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc cagccatatt
    67321 catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc agaatgctgg
    67381 cattaactgg aattgttccg cttgcttctg ccagtaaatc ggcaaggtga tgaatataat
    67441 ccgcccggcc cggtactggc ggacagagaa aaccgtccgg gatatcccag ttcgctacgg
    67501 cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga ttggcaaagt
    67561 ctacgctttg ctccccggcg ggtgtaagcg tgaggaattg cctgagttca ggattgacct
    67621 gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc aaccccggtt
    67681 tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca taacgcgaca
    67741 gggtaaatat ctggctgcgt ttatcatttc taaaaacctt aacatggcta tggtttatgg
    67801 caattcatca taaaaatcag catcatgcat gtcattgatt tgtcttattt tgtaaaaata
    67861 tatcgttcaa aaaataagcg tatctctgtc aattttttgc gtcattttca tcgcccaggc
    67921 gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat gaaaaagtgc
    67981 ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta tgccgcgcaa
    68041 ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc ggcaactggc
    68101 gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga acaaggcgcg
    68161 aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttcggtac tgcaaccatc
    68221 tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc gcctgttttg
    68281 taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt ttcgcgcaat
    68341 gggtgataga aaatcgcgcc aagtgataat gcttatcaaa attattatca cttgcacgaa
    68401 cactatcacg agattaacag tggcatcgca tccgcagaga ggctttctcg tggcagtgaa
    68461 aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc
    68521 agacaatttc attcgctcac gttctttgcc ggtctttgta ttggcatcac gcctgtggct
    68581 caggcactcg ccgccgaagg gcaaactaac gcggatgaca cgctggttgt cgaagcatca
    68641 acgccttcgc tttatgcgcc acaacaatct gcagatccga aattctcgcg tccggtagcg
    68701 gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca gggcgcaacc
    68761 aaccttaccg acgcactcaa aaacgtcccc ggcgtgggtg cgttttttgc gggtgagaac
    68821 ggtaactcca ccactggcga cgccatttat atgcgcggtg ctgatacatc taacagtatt
    68881 tatattgatg gcattcgcga tatcggcagc gtctcgcgcg acaccttcaa taccgagcag
    68941 gtcgaagtga ttaaagggcc ttccggcacc gactacgggc gcagcgcgcc gacgggctcg
    69001 atcaatatga tcagcaagca gccgcgcaat gattccggca ttgacgcctc cgccagtatt
    69061 ggcagcgcct agttccgccg cggcacgctg gacgtcaatc aggtcattgg tgataccacc
    69121 gcggtgcgcc tgaatgtgat gggcgaaaaa acgcacgatg ccggacgcga caaagtcaaa
    69181 aatgagcgtt acggcatcgc cccttctgtc gcttttggcc ttggtacagc gaatcgtttg
    69241 tatcttaatt atctgcatgt cacccagcac aacacgccag acggcggcat tccaaccatc
    69301 ggtttgccgg gctattctgc cccatctgca ggaacggcga ccctgaatca ttccggaaaa
    69361 gttgatacgc ataactttta cggcacggat tccgattacg acgattcgac caccgacacc
    69421 gctaccatgc gttttgagca cgacatcaac gataacacca ccattcgcaa cactacccgt
    69481 tggtcgcgcg tgaagcagga ttacctgatg acggcgatta tgggtggggc gtcgaatatt
    69541 acccagccca ccagcgatgt gaatagctgg acctggtcac gcacggcgaa tactaaagat
    69601 gtgagtaata aaattctcac caaccagacc aacctgacct cgacgttcta taccgcttct
    69661 atcggtcatg atgtcagtac cggcgtggaa tttacccgtg aaacgcagac taactacggc
    69721 gttaatccgg tgacgttacc tgcggtaaat atttatcatc ctgacagcag cattcatccc
    69781 ggcggcctga cgcgcaacgg cgcaaacgcc aatggtcaga cggatacctt cgcaatttac
    69841 gccttcaata cgctgcaaat cacccgtgat tttgagctga acggcgggat ccgtctggat
    69901 aattatcata ctgaatatga cagtgccacc gcctgcggcg gcagcggacg cggtgccatc
    69961 acctgcccag ctggtgtggc aaaaggttct ccggtcacca ccgtcgacac cgccaagtcg
    70021 ggcaatctgg tgaactggaa agccggggcg ctgtatcacc tgacggaaaa tggcaatgtc
    70081 tatattaact atgccgtttc ccagcagcct ccgggcggca acaacttcgc ccttgcgcag
    70141 tctggcagcg gtaacagtgc caaccgcacc gattttaaac cgcaaaaagc caacaccagc
    70201 gagattggca ccaaatggca ggttctggat aaacgcctgt tgctcaccgc cgcgctgttc
    70261 cgtactgata tcgaaaatga agttgagcaa aatgatgacg gaacttactc gcaatatggt
    70321 aagaaacgcg tcgaaggcta tgagatatcc gtggccggga atatcagtcc cgcgtggcag
    70381 gtgattggcg gctataccca gcaaaaagca accatcaaaa acggcaaaga tgttgcccag
    70441 gatggttcct catcgctgcc ctataccccg gagcacgcct ttaccttatg gagccagtat
    70501 caggcaaccg atgatatttc cgttggtgcg ggcgcacggt atatcggcag tatgcataaa
    70561 ggttcagacg gcgcggtggg aacgccagca tttactgaag gttactgggt cgccgacgcc
    70621 aaactggggt atcgggttaa tcgcaatctc gacttccagc taaacgtcta caacctgttt
    70681 gataccgatt acgtcgcctc aatcaacaag agcggctacc gttatcaccc gggcgagcca
    70741 agaaccttct tgctcacagc caatatgcat ttctgattca gatgtggggc gcaggcccca
    70801 ctttttggag aacttgtatg atgtaccaca ttcccggcgt gttatcgcca caggacgtcg
    70861 cccgttttcg cgaacaactg gaacaagccg aatgggtgga tggacgcgtc accaccggcg
    70921 cacaaggtgc gcaagttaag aacaatcaac aggtagacac ccgcagcacg ttatacgccg
    70981 ccctgcaaaa tgaggtgctg aacgcggtta accaacatgc tttgttcttt gccgcggcct
    71041 tgccgcgtac cctttccacg ccgctgttta atcgctatca gaacaatgaa acctatggtt
    71101 ttcatgtgga tggcgcagta cgcagccatc cacaaaacgg ctggatgcgc actgaccttt
    71161 ctgccacgct gtttttaagc gatccacaaa gctacgacgg cggcgaactg gtcgttaatg
    71221 acaccttcgg acaacatcgg gtaaaactcc cggcaggtga tctggtgttg tatccctcca
    71281 gcagcctgca ttgcgtgaca cccgtaaccc gcggcgtacg agtggcatca tttatgtgga
    71341 tccagtcgat gatccgcgat gataaaaagc gcgccatgct gtttgaactg gacaacaata
    71401 ttcagtcgct gaaaagccgc tacggcgaaa gtgaagagat cctgtcgctg ctcaatcttt
    71461 atcataatct gctgcgggaa tggtcggaga tctgacgctt gaactacccg agttcgtccc
    71521 tatattagct acacttaact gtacaagcat tgatatgggg aggacgatat ggcatccggt
    71581 tgggctaacg atgacgccgt caacgaacag atcaacagta caattgaaga tgcgattgcc
    71641 cgcgctcggg gtgaaattcc gcgtggcgaa agcctgtatg aatgtgaaga gtgcggtgcc
    71701 cccatcccgc aagcccgtcg ggaagccatt cctggcgtgc gcttatgtat tcattgtcag
    71761 caggagaaag atttacaaaa accagcttat acaggatata atcgcagagg ttcgaaagac
    71821 agccagttac gttaactaca actggcggga tgtatagcga gtataatttg tatttttcgt
    71881 ggcattccgt gcctttaacg ctgccgtgac tgccacgcga tataaaacgg gtccataagt
    71941 acaacaaata aatggtttat cagtatgtta gatatcaatc aatttatttg aacaaggcgg
    72001 tcaattctct tcgattttat ctctcgtaaa aaaacgtgat actcatcaca tcgacgaaac
    72061 aacgtcactt aaacaaaaaa tcacctgcga gagattaatt atgaaaacta tcaatactgt
    72121 tgttgctgct atggctcttt caactctgtc atttggcgtg tttgccgcgg aaccggtaac
    72181 ggcatcccag gcacagaaca tgaataaaat cggcgtggtt tctgccgatg gcgcatccac
    72241 cctcgatgcc ctggaagcga aactggctga gaaagcggcg gcagctgggg cgagtggata
    72301 cagcatcact tccgccacca acaacaataa attaagcggt actgcggtaa tttataagta
    72361 attcgtagga cggataaggc gtttacgccg catccggcaa cagatgcctg atgcgacgct
    72421 accgcgtctt atcaggccta caaaatccga accgttggac ggatcaggcg tttacgctac
    72481 gtccagcaca agtatttccc tgtctgtttg ccgacagacg catatgctct aaccctcatt
    72541 gatcctatgt tacccttgtt tgcccgtccg ccactggacg ggcttttttt tagctggcta
    72601 actgctgaca gaaagcctgc aacgtctctt ccgacatacc aatctgccgc gccgcatcac
    72661 aaatggcctg ccagcttccc ccatccaatg gaatcccctg cttctgccgt tcgcgacgcg
    72721 tgttcacttc ccactcgccc ggtagcaaaa tcggcttatc gtcatcatgc ggcgaggctt
    72781 tcacccactc ggcaaaggct tcagtctgcg cgctacaatc cggcgcgccg aacagttccg
    72841 ggttgatgat gatagtggtc atgcagttaa gaatggcatc gggactggtt tgtaacgttt
    72901 cctgatgcgt cgttttaccg ccagaaagcg ccccgccaag aatttcacac atcgcggcaa
    72961 gggcgtagcc tttatgttcg gcaaaggtca acagcgaccc caacggcgac tcctgcatta
    73021 ccgccggatt ggtcgtcggc acgccgttaa cgtcaatcag gcaacctggc ggcacgggga
    73081 cgcctttatg ccaggcgacg cgggttttgc caaatgcaat ggcgctggtg gcgtaatcaa
    73141 gcaacagcgg aaaatcatct ttacgtggga aaaccacaca gaacggattg gtgccaaagc
    73201 ggctgtcgcg accgtggaac ggcgcgacca ttggaatacc gaccacgcta acaaagtgga
    73261 tagagacaaa ccccgccgct gcgcactgtt ctgcccagta accgatacgg ccgatatgat
    73321 gcgagttatg tagcgccacg gcagcaatac cgtgctgatg cgctttctca atccccagcg
    73381 ccatcgcttc gtgtgccgcg acctgaccaa atgcgcgatc gccatcgagc gtgaccgccg
    73441 cccccgcctc tttaacgatt ttggcatgat ggttaatttg caggtgcccc tgactccagg
    73501 agcgtacata gcttgggatc atgccaatac catgtgaatc atgccctgcc aggtttgccg
    73561 cgattaaatg atcggcaact aatttcgctt cttgttcctc gctacccatc tgacgaaata
    73621 cagcctgaat aaagctgtgc agcgtctgag catcaaagcg atgaccactt tccatggtta
    73681 actcctgttt atgttatgtg tttgttgttt ttttatgctg cacgccgggc gcgagaaggt
    73741 caataaagat gcaggaattt catattgcgg tggggtaaaa gccggatgac gtggctcatc
    73801 cggctgagat aaaaattaaa atgcctgatt aacgcgcgcc aggccatcgc tgatagttgc
    73861 cgcttcaccc gtagccacca ggcagcaagc catctggatt ttcagcgatt cgggaatcgg
    73921 ttcgctgcca gccaggcaac gctcaatcca ctgcgccgtg gtttccggat cttttgcttg
    73981 tggcagtaac tcgctaccag cagtgtcctg tttttcatac agcacccgca tcccttcacg
    74041 gtcaatgaga ttgatctgcg ggcagcgctg cggattagca tacacttcgc cttcagtacc
    74101 atgcattagt aacgcccgcc cgccgatatc gctaaagaac tttgcgacgc gtccaatgta
    74161 ttccggatgc gaaacgctgg aaagacgcag tgcctcacct tcggcaaatg gcgtcgccag
    74221 tttcgccagg gtatgtgcac tgttacgcac gcccatccgc cagcgcatcg ccagttgttt
    74281 ttccagcggc gggcaaaacg cgccgacagg cataaacacc ggttgatgtt cgtcgagctt
    74341 cgcctgcgcc tgcccgccgt gcaacgttgg cgtaataccc atcagttcga aaatggtttc
    74401 tgtcagcacg cgagttggat cttcgctaac cccgtgaacc accacaggaa aaccgagttt
    74461 gtgcaggaga atcgccaaca acggcgtcag gttggcctgt ttacgcgcgc cgttgtaact
    74521 ggggatgaca atcggcatcg gcttgcctgc aggcggcgtc agcttgatgg tgtgattttg
    74581 catggcttcg taaaagccga gcatctctgc ttccccttcc cctttgatac gcagcgcaat
    74641 caatacgccg cccaactcaa ggtcagggac ttcaccattg agcatatggg catacagacc
    74701 gcgcgcagta tcccggtcta aatcgcgcgc gtggtttttc ccgcgcccga tctctttaat
    74761 gattttgcga tagtccattt acgactcctt acctgactca catcattaac gccgccgacg
    74821 gcgtggggat ttcgtttttt ctttcttttt aactatacct tcagggactt ccggttgctc
    74881 tatcggaaat accggtaatg catccagtag tcgcttgcca taatttttgg tcagcaagcg
    74941 tttatcgtaa atcaccactt cgccccagca accgtggctt cgaatcagtc gcccaacctg
    75001 ctgaatcagg ttaaacgagg cgctcggcag gctttgcacc tcaaacggat agcggttgag
    75061 gcttttcagc cattcccctt cggtgatcac caccgggctg tcgatgggcg gaaaagcgat
    75121 tttgtggata tgcacctggg tgagcagatc acctttcaaa tcaagccctt cggcaaatga
    75181 ctgtaagccc accagcacac tacgctcacc gttggcgacg cgtttgcggt gcagttcaac
    75241 taaacggtaa cgcggctgat cgccctgcac cagcaacatc agacgtaaat ccgtcacata
    75301 gtcgagaaag cgctgcattg cacgcccgct ggcaaacagc accaacatac cgagatgttt
    75361 tttgctctcc acctgctcac ggaaaaaggc cgccatttca gcaatatgct gttcttcgtt
    75421 gtcgatggaa ggctcaacgc gcatccgggg aataacaatt ttgccctgtt cgcagtggtt
    75481 aaagggggaa tccagcgcca caaaacggtc gcccgctttc tctttcagcc cgctcatctc
    75541 ctgcaaacgc gaaaaactgt tcagcgaacg caaggttgcg gaggtgacaa taatgtgcgg
    75601 aatactgcgc cacagcagcc tttccagctg atcgctgaca cgtattccca cgcagtgaaa
    75661 ccagaggtgt agctgccctt cgcgctcttc tcgcgtcgcc catttagtga caggcgcacc
    75721 ggaagattgc gccagcgaag ccagccgcca gagtttgctt tgcgcctcga acatccccaa
    75781 cgcgcggttc atctgcaaaa tcaaccgatg cagacgtaca atgtcatggc tgccggtttt
    75841 ctcacttaaa tcgttaagaa ataactccgc cagaccacgc agcatctcgg tgagttttgc
    75901 cagccgctgg cagatctcca gcacttcatc aggcagttca cccattgcaa aacggtgctc
    75961 tgcctcctgc ccggcaggca tgtagagatt gagaatgttg tttaacgagg cgataagctc
    76021 atacaactct tcacaatgcg cattcaaacg ttcagggatc gccaacggcg ggatggtctt
    76081 cgggcgaaac tgctccatgc aggtagcgac cagtttcgtg aacaagtcca gctgtagccg
    76141 ataccatggc gcggtgattt cggcactcat ctccagcgca tcccgcgcca catctggcag
    76201 gtgatgacct tcgtccagca ccagcagtaa atttttcggg tcaggcaata ccgcttcgct
    76261 ttccatcgcc gccatcacca gcgcatggtt tgccaccacc acttccgctt cctgaatctc
    76321 ccgacgagca acaaaaaacg ggcattcgcg atagtagtaa cagttgcggt tgaggcagct
    76381 ggctttgtcg gtacttaaac gacgccagag atcgtcatct atagcgatat ccgtatgatc
    76441 gcgcagacca tcccatttat aagtgtcgag atcgcccttc agcttcgcac aacgtttttg
    76501 ctcttcctga ttgttcggcg tcagctcgtc gtcaagaaac gccagcagat cctgttgcgt
    76561 gggctcagtg ctggcaagcg ccgtcagatt acgcgggcaa acgtagcgcc cacgcccaaa
    76621 agcggcagtg aatttaagat ctggaatgat ctttttcagg agcggtaaat ctttactgta
    76681 gatctgatcc tgcaacgcca cgttggcggt actcaccacc agcgtttttt gctcttcgcg
    76741 ggcaatggcg atgccgggga tcaaataaga gagcgttttc ccaacgccgg tgggggcttc
    76801 aatcgccaga tgccgccctt cttctccggc cagcgttttg gcgacgtccg caatcatctg
    76861 ccgctgcggc gcacggggaa taaagtcggg gatctgttcc tgaagcgcct tataccaggc
    76921 ggcaatttgc gctttaagcg cggcggttaa tgccatgaga aaacctgaaa tactgtataa
    76981 acagccaata ttgtggcatt tttcggtttt caggggtaat ggtttttctg tttctggaaa
    77041 gcggctcgca aacacgccct ctcgcaatat atttgattga aagttcgacg gcacagtgag
    77101 cgccagtctt cagggcagaa aacagcaagt gataccgcat ttcgtggtta cacttgctgc
    77161 cgtcatcgct tcgcaggaac cggtattgcc cgccatggcc tgagccagga ccatcaatgc
    77221 cattattcag ctgcaatctt tacacccatg cgtctggcgt ccactcggat aaaactttag
    77281 aaaaacgata ttgtgcatag cggaccatat cacgatataa cgaacttata cgcaactggt
    77341 tggcctcttc attagacaga ttatttaaat caagcaatac cgttttgtat atattaaaag
    77401 tttgattgct tatcatactt tcatctaaag aatgtgcttt caccaataaa gcaaatgcat
    77461 aacgttttaa agtttttgac gcatcaggaa taccgttaaa gacactactg tgagaaatat
    77521 ttgcaaatac ggcagacagg cagaaaaata ttttagactt atccctttca ctggcctcgt
    77581 tgagatccag catcgttaat agactagcgt aatgtttatc tttcagagta tgctgttgcc
    77641 attgatctga atcgtttatt aaagaggaaa atgatgaatt aagttctgtt ggttgattat
    77701 tgcattcggg cacaaaatct gtttttcgca ttattgctga cataaatata tcacgaatat
    77761 aacctttgaa tccaagagca ttgaggatca attgatgttt cttatttttc tgcgcatatt
    77821 cccatccttt tgcaaataaa ggaaatgcac gcatacaaat cgtttctgga tcatctatga
    77881 caatttcatg ggtatcctgc aaagatggat aaatagagta attattccag atagtctttt
    77941 tcttaacctc tggctcaagc attgagatta atgcctgttg cgataagcgc atta
//
LOCUS       DAPGRN010000007        74311 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPGRN010000007 DAPGRN010000000
VERSION     DAPGRN010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 74311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.007"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(81..446)
                     /locus_tag="RHW33_000522"
     CDS             complement(81..446)
                     /locus_tag="RHW33_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV1644069.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(739..1725)
                     /gene="yqjG"
                     /locus_tag="RHW33_000523"
     CDS             complement(739..1725)
                     /gene="yqjG"
                     /locus_tag="RHW33_000523"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV1644070.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVEKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1795..2187)
                     /locus_tag="RHW33_000524"
     CDS             complement(1795..2187)
                     /locus_tag="RHW33_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV1644071.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2373..2672)
                     /locus_tag="RHW33_000525"
     CDS             complement(2373..2672)
                     /locus_tag="RHW33_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV1644072.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2662..3066)
                     /locus_tag="RHW33_000526"
     CDS             complement(2662..3066)
                     /locus_tag="RHW33_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV1644073.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3069..3374)
                     /locus_tag="RHW33_000527"
     CDS             complement(3069..3374)
                     /locus_tag="RHW33_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV1644074.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3412..3780)
                     /locus_tag="RHW33_000528"
     CDS             complement(3412..3780)
                     /locus_tag="RHW33_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV1644075.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3927..4310)
                     /gene="mzrA"
                     /locus_tag="RHW33_000529"
     CDS             complement(3927..4310)
                     /gene="mzrA"
                     /locus_tag="RHW33_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV1644076.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4314..4976)
                     /locus_tag="RHW33_000530"
     CDS             complement(4314..4976)
                     /locus_tag="RHW33_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1644077.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5321..6097)
                     /gene="exuR"
                     /locus_tag="RHW33_000531"
     CDS             complement(5321..6097)
                     /gene="exuR"
                     /locus_tag="RHW33_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV1644078.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            complement(6227..7525)
                     /gene="exuT"
                     /locus_tag="RHW33_000532"
     CDS             complement(6227..7525)
                     /gene="exuT"
                     /locus_tag="RHW33_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV1644079.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            8008..9420
                     /gene="uxaC"
                     /locus_tag="RHW33_000533"
     CDS             8008..9420
                     /gene="uxaC"
                     /locus_tag="RHW33_000533"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV1644080.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            9435..10922
                     /gene="uxaA"
                     /locus_tag="RHW33_000534"
     CDS             9435..10922
                     /gene="uxaA"
                     /locus_tag="RHW33_000534"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV1644081.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            11005..11556
                     /locus_tag="RHW33_000535"
     CDS             11005..11556
                     /locus_tag="RHW33_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV1644082.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(11561..12805)
                     /gene="sstT"
                     /locus_tag="RHW33_000536"
     CDS             complement(11561..12805)
                     /gene="sstT"
                     /locus_tag="RHW33_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV1644083.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(13095..13241)
                     /locus_tag="RHW33_000537"
     CDS             complement(13095..13241)
                     /locus_tag="RHW33_000537"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644084.1"
                     /translation="MASKSPKRSLAPDPVAARDKLREPRSGDFVRQSLGGQGAAAIGR
                     PLRQ"
     gene            complement(13350..14315)
                     /gene="alx"
                     /locus_tag="RHW33_000538"
     CDS             complement(13350..14315)
                     /gene="alx"
                     /locus_tag="RHW33_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV1644085.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(14599..15585)
                     /locus_tag="RHW33_000539"
     CDS             complement(14599..15585)
                     /locus_tag="RHW33_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1644086.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKQ"
     gene            complement(15664..16347)
                     /locus_tag="RHW33_000540"
     CDS             complement(15664..16347)
                     /locus_tag="RHW33_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV1644087.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATLFIADRVMVNASKQL
                     TWSDVNVVPARNVGLLLGARPGNRYFTRRIDTAAELYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARISAVIDAKIFHRQPKYLGPSVIIGPF
                     SEHGCPAQK"
     gene            complement(16424..16927)
                     /locus_tag="RHW33_000541"
     CDS             complement(16424..16927)
                     /locus_tag="RHW33_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV1644088.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            17012..18148
                     /gene="rlmG"
                     /locus_tag="RHW33_000542"
     CDS             17012..18148
                     /gene="rlmG"
                     /locus_tag="RHW33_000542"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV1644089.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFIQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            complement(18259..20277)
                     /gene="fadH"
                     /locus_tag="RHW33_000543"
     CDS             complement(18259..20277)
                     /gene="fadH"
                     /locus_tag="RHW33_000543"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV1644090.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYHNRMRFAVEVVSAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELALAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMLILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAEFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGEPQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20803..23154)
                     /gene="ygjK"
                     /locus_tag="RHW33_000544"
     CDS             complement(20803..23154)
                     /gene="ygjK"
                     /locus_tag="RHW33_000544"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV1644091.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFIASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLYLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLNKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLFMLYNDFFRKQ"
     gene            complement(23171..24241)
                     /gene="ygjJ"
                     /locus_tag="RHW33_000545"
     CDS             complement(23171..24241)
                     /gene="ygjJ"
                     /locus_tag="RHW33_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV1644092.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAKGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPEHSDDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASNDPSDQIFLMQYFYW"
     gene            complement(24375..25808)
                     /locus_tag="RHW33_000546"
     CDS             complement(24375..25808)
                     /locus_tag="RHW33_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1644093.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25871..26320)
                     /locus_tag="RHW33_000547"
     CDS             complement(25871..26320)
                     /locus_tag="RHW33_000547"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV1644094.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(26317..29409)
                     /gene="ebgA"
                     /locus_tag="RHW33_000548"
     CDS             complement(26317..29409)
                     /gene="ebgA"
                     /locus_tag="RHW33_000548"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV1644095.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMAGWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSTIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPRSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(29593..30576)
                     /gene="ebgR"
                     /locus_tag="RHW33_000549"
     CDS             complement(29593..30576)
                     /gene="ebgR"
                     /locus_tag="RHW33_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV1644096.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30795..31127
                     /locus_tag="RHW33_000550"
     CDS             30795..31127
                     /locus_tag="RHW33_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV1644097.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            complement(31169..32548)
                     /gene="ygjG"
                     /locus_tag="RHW33_000551"
     CDS             complement(31169..32548)
                     /gene="ygjG"
                     /locus_tag="RHW33_000551"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV1644098.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLIAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32966..34486
                     /gene="aer"
                     /locus_tag="RHW33_000552"
     CDS             32966..34486
                     /gene="aer"
                     /locus_tag="RHW33_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV1644099.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(34593..35216)
                     /gene="nfeR"
                     /locus_tag="RHW33_000553"
                     /gene_synonym="yqjI"
     CDS             complement(34593..35216)
                     /gene="nfeR"
                     /locus_tag="RHW33_000553"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV1644100.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            35504..36268
                     /gene="nfeF"
                     /locus_tag="RHW33_000554"
     CDS             35504..36268
                     /gene="nfeF"
                     /locus_tag="RHW33_000554"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV1644101.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEDGVASSWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHVK"
     gene            complement(36322..36397)
                     /locus_tag="RHW33_000555"
     tRNA            complement(36322..36397)
                     /locus_tag="RHW33_000555"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36362..36364),aa:Ile,seq:cat)
     gene            36522..37028
                     /gene="mug"
                     /locus_tag="RHW33_000556"
     CDS             36522..37028
                     /gene="mug"
                     /locus_tag="RHW33_000556"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV1644102.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEISKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLSIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(37107..38948)
                     /gene="rpoD"
                     /locus_tag="RHW33_000557"
     CDS             complement(37107..38948)
                     /gene="rpoD"
                     /locus_tag="RHW33_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV1644103.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(39143..40888)
                     /gene="dnaG"
                     /locus_tag="RHW33_000558"
     CDS             complement(39143..40888)
                     /gene="dnaG"
                     /locus_tag="RHW33_000558"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV1644104.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     SINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(40999..41214)
                     /gene="rpsU"
                     /locus_tag="RHW33_000559"
     CDS             complement(40999..41214)
                     /gene="rpsU"
                     /locus_tag="RHW33_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV1644105.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            41452..42465
                     /gene="tsaD"
                     /locus_tag="RHW33_000560"
     CDS             41452..42465
                     /gene="tsaD"
                     /locus_tag="RHW33_000560"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV1644106.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(42508..43971)
                     /gene="ttdT"
                     /locus_tag="RHW33_000561"
     CDS             complement(42508..43971)
                     /gene="ttdT"
                     /locus_tag="RHW33_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV1644107.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHTTLSWADWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAEKEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(44019..44624)
                     /gene="ttdB"
                     /locus_tag="RHW33_000562"
     CDS             complement(44019..44624)
                     /gene="ttdB"
                     /locus_tag="RHW33_000562"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV1644108.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(44621..45532)
                     /gene="ttdA"
                     /locus_tag="RHW33_000563"
     CDS             complement(44621..45532)
                     /gene="ttdA"
                     /locus_tag="RHW33_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV1644109.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATIKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLSFENLSHTRSAL"
     gene            45739..46671
                     /gene="ttdR"
                     /locus_tag="RHW33_000564"
                     /gene_synonym="dan"
     CDS             45739..46671
                     /gene="ttdR"
                     /locus_tag="RHW33_000564"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV1644110.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            complement(46684..47301)
                     /gene="plsY"
                     /locus_tag="RHW33_000565"
     CDS             complement(46684..47301)
                     /gene="plsY"
                     /locus_tag="RHW33_000565"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV1644111.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            47406..47774
                     /gene="folB"
                     /locus_tag="RHW33_000566"
     CDS             47406..47774
                     /gene="folB"
                     /locus_tag="RHW33_000566"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV1644112.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47865..48686
                     /gene="bacA"
                     /locus_tag="RHW33_000567"
     CDS             47865..48686
                     /gene="bacA"
                     /locus_tag="RHW33_000567"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV1644113.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48867..50105)
                     /gene="cca"
                     /locus_tag="RHW33_000568"
     CDS             complement(48867..50105)
                     /gene="cca"
                     /locus_tag="RHW33_000568"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV1644114.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(50169..50789)
                     /locus_tag="RHW33_000569"
     CDS             complement(50169..50789)
                     /locus_tag="RHW33_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV1644115.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            51031..52332
                     /gene="ygiF"
                     /locus_tag="RHW33_000570"
     CDS             51031..52332
                     /gene="ygiF"
                     /locus_tag="RHW33_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV1644116.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            52355..55195
                     /gene="glnE"
                     /locus_tag="RHW33_000571"
     CDS             52355..55195
                     /gene="glnE"
                     /locus_tag="RHW33_000571"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV1644117.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWQQLTGALTGHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMALPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            55243..56676
                     /gene="hldE"
                     /locus_tag="RHW33_000572"
     CDS             55243..56676
                     /gene="hldE"
                     /locus_tag="RHW33_000572"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV1644118.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56796..56855)
                     /gene="ibsE"
                     /locus_tag="RHW33_000573"
     CDS             complement(56796..56855)
                     /gene="ibsE"
                     /locus_tag="RHW33_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV1644119.1"
                     /translation="MMKLVIILVVLLLLSFPTY"
     gene            complement(57172..57231)
                     /locus_tag="RHW33_000574"
     CDS             complement(57172..57231)
                     /locus_tag="RHW33_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1644120.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(57470..59131)
                     /locus_tag="RHW33_000575"
     CDS             complement(57470..59131)
                     /locus_tag="RHW33_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV1644121.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGTAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(59158..59787)
                     /locus_tag="RHW33_000576"
     CDS             complement(59158..59787)
                     /locus_tag="RHW33_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV1644122.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            60056..60256
                     /gene="glgS"
                     /locus_tag="RHW33_000577"
     CDS             60056..60256
                     /gene="glgS"
                     /locus_tag="RHW33_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV1644123.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            complement(60291..60590)
                     /gene="ubiK"
                     /locus_tag="RHW33_000578"
     CDS             complement(60291..60590)
                     /gene="ubiK"
                     /locus_tag="RHW33_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV1644124.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNSVADLQSPPAIPPIDKAE"
     regulatory      60712..60860
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            60964..61617
                     /gene="ribB"
                     /locus_tag="RHW33_000579"
     CDS             60964..61617
                     /gene="ribB"
                     /locus_tag="RHW33_000579"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV1644125.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSTYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            61879..62049
                     /locus_tag="RHW33_000580"
     CDS             61879..62049
                     /locus_tag="RHW33_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644126.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(62107..62880)
                     /gene="zupT"
                     /locus_tag="RHW33_000581"
     CDS             complement(62107..62880)
                     /gene="zupT"
                     /locus_tag="RHW33_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV1644127.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            63023..63811
                     /gene="ygiD"
                     /locus_tag="RHW33_000582"
     CDS             63023..63811
                     /gene="ygiD"
                     /locus_tag="RHW33_000582"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV1644128.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQHLVELLAPVPVT
                     LDKEVWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(63849..65009)
                     /locus_tag="RHW33_000583"
     CDS             complement(63849..65009)
                     /locus_tag="RHW33_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV1644129.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(65015..65686)
                     /locus_tag="RHW33_000584"
     CDS             complement(65015..65686)
                     /locus_tag="RHW33_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV1644130.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(65834..67315)
                     /gene="tolC"
                     /locus_tag="RHW33_000585"
     CDS             complement(65834..67315)
                     /gene="tolC"
                     /locus_tag="RHW33_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV1644131.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            67520..68149
                     /gene="nudF"
                     /locus_tag="RHW33_000586"
     CDS             67520..68149
                     /gene="nudF"
                     /locus_tag="RHW33_000586"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV1644132.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            68150..68572
                     /locus_tag="RHW33_000587"
     CDS             68150..68572
                     /locus_tag="RHW33_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV1644133.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            68597..69424
                     /gene="cpdA"
                     /locus_tag="RHW33_000588"
     CDS             68597..69424
                     /gene="cpdA"
                     /locus_tag="RHW33_000588"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV1644134.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            69424..70005
                     /gene="yqiA"
                     /locus_tag="RHW33_000589"
     CDS             69424..70005
                     /gene="yqiA"
                     /locus_tag="RHW33_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV1644135.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            70034..71926
                     /gene="parE"
                     /locus_tag="RHW33_000590"
     CDS             70034..71926
                     /gene="parE"
                     /locus_tag="RHW33_000590"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV1644136.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(71990..74131)
                     /locus_tag="RHW33_000591"
     CDS             complement(71990..74131)
                     /locus_tag="RHW33_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDV1644137.1"
                     /translation="MAKFTPSFSGIKGRALFSLLFAAPMIHATDTATTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPTSTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLIRRVDATQGSTQRMHSTRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KNAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
BASE COUNT        17650 a        18980 c        19732 g        17949 t
ORIGIN      
        1 aataaacatt agtaaattat tcattcatca acatgatgat aaaagccgga tgagtcagac
       61 atccggcgag cagatttgtt ttaaggttcc agttttggat caggtccaaa gcggttttcg
      121 cctggcgtac ccgcctggca gttaaagacg atgataataa gccagccgat aaacgggatt
      181 aagaatagca gtgcccacca cgccgagcgg tcggtgtcgt gcaggcggcg gaactgaact
      241 gcccaccacg gtaaaaacac cagaataccg tagattgtcg tcaggatgcc ttccccacca
      301 gcgcgttgcc agcctaacat tttatccagc agccccagca cgaacgtaaa gatgatgttg
      361 accagaataa acatccagta ctctttgcgc cgcgcacgac cccggaaacc gacataattt
      421 tttagtactt tcagatacca gtccattttt gcctcaattt tgctcaatca cttgttttta
      481 aagcagcaaa ttaagaatag acgtgaatga aatggcagta aatattcgtt caaggaatta
      541 ataaagtgct gtgaaggcgc gctaagactg atgacaagcg caaaatcgcc agatgcgcta
      601 cgcttatctg gcctgcgcgg tcattgcaac atattgaata taaatgtttt gtaggtttga
      661 taaggcgttc acgccgcatc cgacatgaac aacatgcact tagccaatta tcagaggcac
      721 ctcgtaaggt gccttttttt aaccgaagcg aacatctcgt ccatgcggtt catcgagatc
      781 ctgccacgga ccaattgaaa taatccccgt agggttgatg gtcttatggc tgcggaagta
      841 atgattacgg atgtgatcga aattgactgt ttcggcaatt cccggcatct ggtagatatc
      901 acgcaggaag ccatacagat taaggtaatc gctgatgcgg tgcttatcac acttgaagtg
      961 ggtcacatac actggatcaa aacggaccag cgtggtccac aggcgaatat cggcttcggt
     1021 tagctggttg ccggtcaggt aacgatgctg acctaaaatc tgttccagtc gcgccagcga
     1081 ttcaaacact ttctccaccg cttcgtcgta ggcttgctgg ctggtggcaa aaccagcttt
     1141 atacacgccg ttgttaacgg tgtcataaat ccagccgtta agttcgtcaa ttttcgtttg
     1201 cagggctggt gggtagtaat cacccgcttt cgcgcccagc gcatcaaacg cggtattaaa
     1261 catgcggatg atttctgctg attcgttgct gacgatggtg tggttctttt tgtcccacag
     1321 cacgggaaca gtaactcgtc cgctgtagtg tggatcggcg tggagataaa gctgatacaa
     1381 aaattcatgt tgatagagcg tgtctccggt tgctcccgga aaactgtcat caaaggtcca
     1441 gccgttttcc agcatcagcg ggttcactac ggaaacggaa ataaacggtt ccagtccttt
     1501 gagtttgcgc atgatcagcg tgcggtgcgc ccacgggcag gcgagtgaaa catagagatg
     1561 ataacgatct ttctctgcga taaaaccgcc tgtgccagtg gggccaggag cgccatcggc
     1621 agtgagccag ttacgaaatg cggaagctga acgttgaaat ttaccgccgg tagatttggt
     1681 atcgtaccag gtgtcatgcc agacgccgtc aatcagttga cccattgttc tctcctccaa
     1741 tagcaaaaac gaggagcttc ctcctcgttt tttatcgtta agtatagcgc gtgcttacca
     1801 ctttttattc agcaggcggt cgatgctata cgcgcccgga ccggtaattg ccagcagcag
     1861 gaatccgcca gaaattgtca ggtttttcat gaacatcagc gagttgacgc cttcagcaaa
     1921 gttgctgtga aataaaaatg ccgtcagcag cgtaaagccc gcagtaaaca gggctgtggt
     1981 gcgagtcagg aaaccgaaca ggattgccag accaccacca aactcaagca gaatcaccag
     2041 cggcagcata aaacccggga cgcccatcgc ttccatatat tgttgggtac ccgcgtaacc
     2101 agtaattttt ccccagcctg cggtaataaa cagaatcggc attaaaatgc gcgctaccag
     2161 tacaccaaca tcttctaatt ttttcatcat actctccact gcgccactca ggctgctgat
     2221 tgtttttttg tgcaagggcg cggtattagc gtcgttgctg tcgatggaga gaatcataaa
     2281 cgtggtgaat gatgattgtt agcaaggaaa actgtcaaaa atcttcaaaa aatttgatgg
     2341 gtaaggccgg aatggctccg gccagaggga agttaaccgc gaagctgttg ctgcttgagg
     2401 gtcgttttaa ccagacgcca ggcgctccat acgccaaaac cgcgtctggc ccagcggacc
     2461 agcatattag gatggcgaat cgtccagatc gccatcacgc tactgccaac cagcgcccag
     2521 gagcgcagac ttagcagcat attccagcga cgatcgtaag cgcctgttgc ctccagccat
     2581 tcacgacgac tggcagaaag atccagccgt tgttgctgga tctggctaag cagttgcgcc
     2641 ttacgtcgtt cacgttcgac tttactgctc acgggactcc tcctcgagca gctgccgatc
     2701 gtttgccagc tcatggcgtg tatggcgcag caaggtggat ttacgcgatt tacgtaacgt
     2761 ccagataccg ccaatcagtg ccagtagcag caacaccacg gtggtggcaa tcatcgcatt
     2821 caggcgatat tgcggatcaa ccgcccaaat aattagcacc atcaggctca taagaccaaa
     2881 tgcagcgaaa agcatcgtca ggcccagcat cagtaaaagt tgaaagagat tagctttttc
     2941 ctcttccagc tcaaccaccg ccagccgcag acgtgtttcc accatttcaa ccatgatgga
     3001 aacaattcgc tgcccgatgc ccagaacgct tttaccgggc ccttgcgcgt gatgagtgtc
     3061 cgccataatt aacgacgcga cagcagaacg ccgagcacta caccgattgc agcgccaatg
     3121 cccacgcccg tccatggatt ttcacgcaca tactcatcgg cacgcgccgc cgcgacacgg
     3181 gtttgtttgg cgatggcatc accggtttca cccaggcgat aacggctctg tttcagtgcc
     3241 tgctccgctt tgctacgaat cttactcaac tcttctttcg acttctcgcc agatgagcta
     3301 agcacctctt ccagcgtatc ggaaagggat ttcaactcag cacgcagatg ttccgtagtg
     3361 tgttctttcg acatagtttt ctccaggtga gtaagtagtg actattgtga gttagtagtc
     3421 gcgcgcttcc agctttttca gctcttcctg cgcttctgcc agtttccgtt cgcgtttggc
     3481 aatcttatcg gcatcgcctt tttgcttcgc ctcggctaaa tcttgctggc gttccgccac
     3541 ctcatctttc tgctttgcga ttttcttctg atgatcggca cgcagctggc tatctgaaca
     3601 gttggcccgg acttcactca gggctttatt cagaccgtca atacgattct ggttttggtg
     3661 tttttcggca tagctgattt ccttaaggat attttgctcc ttttcctgac acagggtagt
     3721 ggcataacta ccggcactaa gagcaaaaag agaaacagct aaagcgatgc ggtatttcat
     3781 tcttgaacct tccattgtga ttcggaccta ttacaaagga acgattgtcc agacaacgaa
     3841 gcggtagtgt taaccaccgc ctcattaatc atagacagtt acggtgaaat caccaaagtg
     3901 ggtgatctgt ttcggataac aggaagttat ccgaagcgat gagagttatc ccgtagccgg
     3961 gtcagccact gcatagcctg actattattg tcctgctgcg caatgatgta gccatggggc
     4021 aatgttctgt cgaggaccgc ttttgcggcg gcgctctggt cgctggagtc aaaggtaatt
     4081 aacagagtgt cgtttttggg ggtgatactt ttgaaaggaa ttccgtgagc atcaaggtga
     4141 tgccagatcg aaaaaccgtc tggcatcgtt gtgccttgat gaacggcacg aatcgccagc
     4201 gtagactctt gctggcgaac cgcggaccag gccagcaaca gcgtgccgac cagaagtaaa
     4261 acagcgccgg accaggctag ctggcgaagc gacatgcgag gtatttgcat cccttacccc
     4321 cgatttccat atttcttttt ccataacacg accagagaac ctgccaggcc aaaaaccagc
     4381 agcaccaccg ggagcagcat caggcatgac atcagctggt cctcgtactt taaaaatacc
     4441 ggcgttttgc cgagcatgta acccagagtt gtcaggatca atacccacag cagaccgctc
     4501 atccagttga aaaactgaaa gcgcgcgtta ttcagccctg ataacccggc aatcgtcggc
     4561 agcagtgttc tgacaaacgc aataaagcga ccaattaaca gcgccgataa accgtgttta
     4621 tgaaaaagat ggtgtgcgcg ttgatgataa tgcgcgggta aatgagatag ccagttttgt
     4681 acggtgcggg tattgcccag ccatcgcccc tgaatatagc tgacccagca gccgaggctg
     4741 gcggcaacgg tcagcagcag aatcgtttgc ggatagccca tcgcgccttt cgcaatcaac
     4801 acgccgacca ataccagtaa actgtcgccc ggtaaaaagg ccgccggaag caagccgttt
     4861 tcaaggaaca aaattacaaa caagacaaaa tacaacatgc caatcatcga tggattggcc
     4921 agggtttcaa aatcctgcgc ccacagggct tgcagcaatt gggtcaaaag ttccattcat
     4981 tattcctggt gaatcggtta acgccacgct ggttttaaaa gatgcactac cgcaatgtta
     5041 ttgctgtttt ggtatgttct gcgggtttga tacatcaaca cagcctgaac tattccctgt
     5101 taaatcgtga gataagaaag cattcaaaaa cataaattca aattgaatct aattgtaaca
     5161 aaggtcaacg ttgttcgtta tcaaaattac atgtccgtgg agtctgattt tgctgaatgc
     5221 aggggagggt ggcgagagga tttacacagg ctgacacttt atacttttcg cttaccgacg
     5281 agacaaacag gcgcgtcgca gcgcctgtcg gacaggtatt tcatttactg ccactggtgg
     5341 ccgtatcgag atgcactacc gggttttcgg cgaacagata acggtcggca ttgaactcga
     5401 agtcgtcgct ggtttcgtta aataacatga tcttggtgtt ttccaggtgc tgccacattg
     5461 ccagcttagc agcatgagga tctttgcgaa tcagcgcttt gaggatttga tcgtggtcat
     5521 cacaccagtt atcgacggta cgggaatcaa tgtgttcatg cagttttttc cagtacgggt
     5581 tatggctacg ctgggtccac attttttcaa cgatagccgc cagggcggag ttctgcgtcg
     5641 ccagggctac ctgaatatgg aactgcaaat cccactcgga atcacggaag cattgttcac
     5701 cgcgtgcctg ctcctggatg gccatcaatt tcatgatgtc ctgtttcgtt acctgagttg
     5761 ccgcgaactc ggcaatatta ctttcgatga gctggcgagc ctggagtaac tcaaacggac
     5821 cgtaattggc gaattccata ttattgtccg ccgcctgctg atggcgcggc tggttggaaa
     5881 ccacgtgaat acctgaacct ttacgcactt cgacatagcc ttcaacttcc agcatgatga
     5941 tggcttcacg aactaccgta cggctgacgt tcttttcatc ggcaataaag cgctcggcag
     6001 gcagtttatc acccaccaga tagacgccct gttcgatgcg ctctttcagg tcagcggcaa
     6061 gttgttgata caaacggcgt ggttcagtga tttccatatg cgctccaggc atgatacggc
     6121 agactatatt tgttatacca cttttgcgag tttagctcta ctcgcagtga tgaaaaagcc
     6181 gcctgagaag gcggctttga agggaaacta aaacttacac gaagcgttaa tgttgcggtg
     6241 cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg tttgttctgc aatacggtcc
     6301 agataaccag cgcacccagc aggtcaaata ctgccagaac tgcgaacagc gggctgaagc
     6361 cgatggtgtc agccagtgca ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg
     6421 acatcccggt taaaccgttt gccgttgcca cttcgttacg accaaacacg tcagaagaga
     6481 gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc accgatacac agcagcataa
     6541 ttgcgacata cgggttggtg aacagaccga tcatacccgg gccaatcatc agcacagcac
     6601 ccagcgttac gaccatctta cgggaaacga tcaggttcac accaaaccag cgctggaaca
     6661 gcggcggcag gtaaccaccg aggatacaac cgaggtcagc aaacagcatc ggcatccagg
     6721 cgaacatcgc gatctctttc aggttaaagc cgtaaacttt aaacatgaac agcgggatcc
     6781 acgcgttaaa agtaccccag gctggttctg ccaggaaacg tggcagcgcg ataccccaga
     6841 actgacggtt acgcaggatc tgaccaacgg acattttctt cgccgtactc acttggtgct
     6901 gggcttcctg accattaata atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc
     6961 gcggatgttt atagaaaatc agccatgcca tcgcccagat aaagctcaat gcaccggaga
     7021 tgataaatgc catctgccag ctgtgcatca cgattgccca taccaccagc ggcggcgcaa
     7081 tcatcgcacc aatcgaagaa cctacgttaa agtagcctac tgcgatggaa cgctctttcg
     7141 ccgggaacca ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac
     7201 cgaccgcacc acgagcaaca gccaggccac cccagctacc tgccagcgcg gttgcaccac
     7261 agaacacggc ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca
     7321 gtacataacc cgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat
     7381 aggaatactg ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca
     7441 cagtgttacg cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg atcatatacc
     7501 aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag
     7561 cgcggccatc atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa
     7621 accggcacgc cccgaacgtt gccatgaaag tttcgtgcta attcggcttc cgtaccggtt
     7681 aacccgattc atctaacaaa tgagtgaatc ggcaatttgc attccgtcga cttatgattt
     7741 gcgacggcag aaagataact tgtcatacaa ctttaaaagg tgagagccat cacaaatgtg
     7801 cgaatatttg tagggacatt acctgacgac agcgaggcca gtactggcgc ggcctgcagc
     7861 gagatttacc actttgagag taattttttt aactacgttt attgatctaa ctcacgaaaa
     7921 tatcttcgga ctctggaaat tggtgtgata actttgtcag catcgcacca taagcaagct
     7981 agctcactcg ttgagaggaa gacgaaaatg actccgttta tgactgaaga tttcctgtta
     8041 gataccgaat ttgcccgccg tctgtatcac gactacgcaa aagaccagcc gattttcgat
     8101 taccattgcc atttgccgcc gcagcagatt gcggaagact atcgttttaa aaacctgtat
     8161 gacatctggt tgaagggcga tcactacaaa tggcgcgcta tgcgtaccaa cggtgtggct
     8221 gagcgtctgt gtactggtga tgcgtctgac cgtgaaaaat ttgacgcctg ggcggcgact
     8281 gttccgcaca ctatcggcaa cccgttatac cactggacgc acctcgaact gcgtcgtccg
     8341 tttggtatca ctggcaaatt gctttctccg tcaactgccg atgaaatctg gaacgaatgt
     8401 aacgaactgc tggcgcagga taacttctcc gcgcgcggca tcatgcagca gatgaacgtg
     8461 aaaatggtcg gcaccaccga tgacccgatc gattctctgg agcatcacgc agagatcgcc
     8521 aaagacggct ctttcaccat caaagtgctg ccgagctggc gtccggacaa agcctttaac
     8581 atcgaacagg cgacctttaa cgactacatg gcgaagctgg gcgaagtttc cgataccgac
     8641 attcgccgct ttgctgacct gcaaactgcc ttgaccaaac gtctggatca cttcgccgct
     8701 cacggctgta aagtgtctga ccatgcgctg gacgtggtga tgtttgctga agcgaacgaa
     8761 gcggaactgg acagcattct ggcgcgccgt ctggctggcg aaaccctgag cgagcacgaa
     8821 gtggcacagt tcaaaactgc ggtgctggtg ttccttggtg ccgaatatgc acgtcgcggc
     8881 tgggtacagc agtaccacat tggcgcgctg cgtaataaca acctgcgtca gttcaaactg
     8941 ctggggccgg atgtaggctt tgactccatc aacgaccgtc cgatggcgga agagctgtct
     9001 aagctgctga gcaagcagaa cgaagaaaac ctgctgccga aaaccattct ctactgcctg
     9061 aacccgcgcg ataacgaagt gctgggcacc atgatcggta acttccaggg cgaaggtatg
     9121 ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg atcagaaaga cggtatggaa
     9181 cgtcagatga cccaactggc gcagctcggt ttgctgagcc gctttgtcgg tatgctgact
     9241 gacagccgta gcttcctgtc atacacccgt cacgaatact tccgccgcat tctgtgccag
     9301 atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg acatcaacct gctgggcgag
     9361 atggtgaaaa atatttgctt taacaatgcg cgtgactact tcgccattga actgaactaa
     9421 ggtctggggt tgatatgcaa tacatcaaga tccatgcgct ggataacgtc gcggtcgctt
     9481 tagcagattt ggctgaaggc acagaagtca gtgtcgataa ccagactgtt acgctgcgcc
     9541 aggatgttgc tcgtggacat aaatttgcgt taacggatat cgcaaaaggg gccaacgtca
     9601 tcaaatatgg cctgccgatt ggttatgcat tggcggatat tgcggcgggg gaacacgttc
     9661 acgcccacaa tacgcgcacg aatctgagcg atctggatca gtatcgctat caacctgatt
     9721 ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca gatctatcgt cgcgctaacg
     9781 gcgatgtcgg ggtgcgtaat gagctgtgga tcctgccaac cgtgggctgt gtcaacggca
     9841 tcgcgcggca gatccaaaat cgtttcctga aagagaccaa caacgccgaa ggtaccgacg
     9901 gcgtgttcct cttcagccac acctacggct gctcacagct gggcgacgat cacattaata
     9961 cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc aggcgcagtg ctggtgattg
    10021 gcctgggctg tgaaaacaac caggttgccg cattccgtga aacgctgggc gatatcgatc
    10081 ctgaacgcgt tcatttcatg atctgccaac agcaggatga tgagatcgag gccgggatcg
    10141 agcatttgca tcagttgtat aacgtgatgc gcaacgataa acgcgagcca ggcaaactca
    10201 gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg tctttctggt attactgcta
    10261 acccgatgct ggggcgtttc tctgactacg tgattgctaa cggcggtact accgtactga
    10321 ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat ggaccattgc cgcgacgaag
    10381 caacgtttga aaaactggtc actatggtca acgacttcaa acagtacttt attgcccatg
    10441 accagccgat ctatgaaaac ccatcgccgg ggaacaaagc gggcggtatc accacgctgg
    10501 aagacaaatc acttggctgt acccagaaag cgggttccag cgtcgtggtt gacgtgctgc
    10561 gttacggcga gcgtctgaaa acgccggggc tgaacttgtt aagtgcgccg ggtaacgatg
    10621 ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat ggtgctgttc agtactggtc
    10681 gtggcacgcc gtatggtgga tttgtgccga cggtgaaaat cgccaccaac agtgaactgg
    10741 cggcgaagaa aaaacactgg atcgactttg acgcgggtca gctgatccac ggtaaagcga
    10801 tgccgcagtt gctggaagaa tttatcgaca ccatcgttga gtttgccaac ggtaagcaaa
    10861 cctgtaacga gcgtaacgac ttccgcgaac tggcgatctt caaaagcggc gtaacgctat
    10921 aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata atgtgaagcg ccgtttgctt
    10981 ttccatacct gctaaggatt tatcatgacc gcgtattggc tggcccaggg cgtgggtgtc
    11041 atcgcctttc tgattggtat cacaacattt ttcaatcgtg acgaacgtcg cttcaaaaag
    11101 cagctttcgg tctatagcgc cgttattggc gtacattttt ttcttctggg cacctatccc
    11161 gctggtgcca gtgccatcct taatgccatt cgtacattga ttaccttacg cacgcgcagc
    11221 ttatgggtaa tggcgatttt tattgtgctg actggcggaa ttggcctcgc gaagttccat
    11281 catcctgtcg aactattgcc ggttatcggt acgattgtca gtacctgggc gctgttccgc
    11341 tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa cgtgttgctg ggtgattcac
    11401 aacttctggg cggggtcgat aggcggcacg atgattgagg gtagttttct gcttatgaat
    11461 ggcctgaata tcattcgttt ctggcggatg cagaaaaggg gaattgatcc gtttaaagta
    11521 gagaaaaccc ctcccgccat agacgaaagg ggttaaacaa ttaattacgc agggcgctat
    11581 ttgccagacg atcgtcttct gcctggcaag ctgccgcagt gaatagcacg tcagttgaag
    11641 agttcagcgc ggtttcgcaa gagtcctgca atacgccgat gataaagccg acggcaacca
    11701 cctgcatggc gatatcgttc gaaataccga acatattaca ggccagtggg atcagcagca
    11761 gagacccccc cgccacgccg gatgcgccac aggcacacag agaagccact acgctcaaca
    11821 gcagcgctgt gggcagatcg accggaatac ccagcgtatt aaccgcagcc agcgtcaaca
    11881 cggtaatggt gattgctgcg cccgccatat tgatggtggc acccagcgga atagaaacgg
    11941 aataggtatc gcgatccaga ttcagctttt cacacagcgc catattcacc ggaatgttag
    12001 ctgcagagct gcgggtgaag aaggcataca cgccgctttc gcgcaggcac agcagcacca
    12061 gcgggaacgg gttacgacga attttccacc acaccagcaa tgggttaacc accagcgcca
    12121 ccagtaacat acagccaacc agcacgacca gcagttgcgc gtagccccac agtgtggaga
    12181 aaccggtggt tgccagcgta gaagaaacca gcccaaaaat accgatcggt gcgaagcgaa
    12241 tgaccagttt caccataaag gtgacggcat tcgacatatc gttgaccagg tttttggtgg
    12301 tttcgttacc gtgacgcagt gcgaaaccga ggccaattgc ccacaccagg atcccgatgt
    12361 agttaccttt cagcagcgcg tcaatggggt tggaaaccat gctcattacc agtccgcgca
    12421 tcacttcgac aatgcctgac ggcggcgaaa tatcacccgc gctactggac aagtgcaggg
    12481 tagaagggaa ggcaaagctg aagactactg cggccagagc tgcagagaag gtgcccagca
    12541 gatagaggaa caaaatagga cggatattgg ttttctgccc gtgctggtgg ttagcaatag
    12601 atgccattac cagcatcaac accaggatgg gggcaacagc tttcagtgcg ccgacgaaca
    12661 aagtacctaa cagaccaaca gcttccgccg cgggttttga gatccatgcc agaagaatcc
    12721 ccagaacaag gccgaccagg atttgtttta ccaggctgcc atgagccaga cgccggaata
    12781 gccccggtga acgttgcgta gtcatttttc gatcctttca ttgtgttgtc gcacatccct
    12841 ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt gtttgcaagg tcgagtataa
    12901 ggaaagttac agcttcggga agcgaaaaat gctggatttt aaagagaggt catattttgc
    12961 aacttaatgg ttacatttat ttgacataaa cagagggaag gactggctac taaggctgat
    13021 tagataacat atgggcaaaa ctatttggaa tatgagaacg gttgcgctcc tttaatgtgc
    13081 tatgccatat aaagtcactg gcgcaggggg cggccaatcg ccgccgcccc ctgccccccc
    13141 aggctctggc gaacaaaatc gccgctgcgc ggttccctca gcttatccct tgcggctacc
    13201 gggtcgggcg caagggaacg cttcggtgat tttgacgcca ccaacacccg tgcagtcttt
    13261 ttgtaaagag ctataagccg attacataat taatagctca cctttgtctg gaatgagtta
    13321 tctctagtaa tggcttaata tacagcgaat cattctcccc gctgcttatc atgccgataa
    13381 ttcacccagg cgttgataat aaacgtcatc accaggatgc caaacaccac gcccagcgag
    13441 acggcgattg gaatatggta gaagtcgaca atcagcatct tgataccgat aaataccaga
    13501 atcaccgcca ggccatattt gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa
    13561 tacatcgcac gcaggccgag gatcgcaaac aggtttgagg tcagcacaat gaacgggtca
    13621 gtggtcacgg cgaagatagc cggaatgcta tccacggcaa aaatcacgtc gctcaattcc
    13681 accagaatca gtaccagcat cagcggtgtg gcgtacagca atccattctt acgcacaaag
    13741 aaatgctcgt tgtcgatggt gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc
    13801 ggcttgtcgc caatgcctga ttcatcttca tgggcgagcg ccatcttcac gccggtaaac
    13861 agcaggaagg caccgaagat atacaggatc cagtcgaact gcgaaatcag ccagctgcct
    13921 gtgaagatca tgatggtacg cagaacaatc gccccgagca ctccatacac cagcacgcga
    13981 cgttgtaatg ccgccggaac ggagaaatag ctgaacaaca tcagccagac aaagacgtta
    14041 tcgaccgcca gcgatttctc aatcagataa cccgtgagaa aggccagtgc ctgtggatcg
    14101 gcaacggcgc gaccttcggt ttgcaccaga taccaccaga aagcggcgtt aaacagtaac
    14161 gacagcgtca cccagaccag cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc
    14221 ccacgacgcc cctgcaacaa caggtcgata gccagcataa tggcgacaac aacagcgaat
    14281 ccgccccata gcaacggcgt gccgacagta ttcatagaag ttccttacac ataaaaaaaa
    14341 cggccaacgt cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa
    14401 ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac
    14461 cggatgtgat ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt
    14521 gcaggtaaca aagtatgaca gaccattcac ccagtcaatg gtctgttaca tgccttttac
    14581 actgctttac ggtaacaatt attgttttac gctatctgcc ggaaaaatca cccctgtctg
    14641 gcggcggatc tctgtcagca atttggcggt gatgcgactg accgccagtc ccggatgatc
    14701 caccagatgc tcatccacca gggtagcgaa cagctctgct tcgtagagca tggtattaat
    14761 atgctgcggc tgggtgagat cctgcatctg gctgccacgc ggcacaaagc atactttctg
    14821 gcattcagac agtttttcaa tcaccagcga tcccgcttcg ccctgaattt cgcttgccag
    14881 gacagaatca ctgacttttg agtgctgcaa cgtaacgctg aaatcaccgt aatccattac
    14941 caccacgccg tgggcatcta cgccgcttgc cagcaaactg gcggttgcct gcacgctttt
    15001 cggctcacca aataacgcca ccgccgacgc caggcagtaa aagccgatat ccataattga
    15061 accgttagag aatgccggat taaaggtgtt gggattctca ccgtccaggt aacgttgata
    15121 ccgcgaggaa tactggcaat agttgaaaaa aactttccgc agtttgccga ctttcggcag
    15181 cgcctggcgc aacaaatgaa agttcggcag gctggcggtt ttaaatgcct caaacagcac
    15241 cacctgattt tcccgcgcac aggcaatggc ggcatccact tccgccagat tcgacgccag
    15301 tggtttctcg caaatcacat gaattttatg gctaaggaaa agttgtgtct gggaaaagtg
    15361 cagggaattc gggctggcaa tatacaccgc gtcaatggca tcgctttctg ccatcgcttc
    15421 cagcgacgta aacagatgct cgacagaaaa atcattggcg aagtgctggg cctgttcaag
    15481 gctgcgggaa tatacggcgg ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa
    15541 ctggcgagtg atccagttcg taccaatcac agcgaaacgt atcataacgc gtggatactc
    15601 cataaaggga ttctgccgtc aatgtagcac gcctttttca tcttcgacgt gcgcggatac
    15661 gcattatttc tgcgctggac aaccatgctc actaaacgga ccaataatga ccgacggccc
    15721 gagatatttt ggctgacgat ggaagatttt cgcgtcaatc accgcgctga ttctggcgag
    15781 tttctcccgt aactgagtat aaaaaccgtg cttcatattc aggtctggtg cgttaaaccc
    15841 gatagcatcg atgccgtatt gttttgccag ccagatggcg cgttgattat gaaattcctg
    15901 cgagataatg gtgatatggt tttccccaaa cacctttttg gcacgcacta ccgaatccag
    15961 cgttgagaat ccggcatagt cgcagaagat cactttcgcc ggtacgcctt tagcgatcaa
    16021 agcctgctgc atacctgatg cttcatcgta atttttacgt ccgttatcgc cactgactaa
    16081 cagccatttc acttttccgg cgtggtacaa ctctgctgcg gtatcaatac gtcgggtaaa
    16141 gtagcgatta cccggcctgg cccccagcaa taagcccacg ttacgcgccg gaacgacgtt
    16201 gacgtcgctc caggtcagct gtttgctggc gttgaccatt acgcgatcgg caataaaaag
    16261 ggtcgctcct gccacaagta acagtaagca ggcaatcttt agagtccggc gagaaaaaca
    16321 aattcggaga agaaggcggg gctgcatggt cgttatcctt aacgaaatgg gggaggtcga
    16381 ttttaggcgt taaaggaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc
    16441 caagcgacaa ctgggttagc cacagtcggg tgtcgaactc aagctggtgg tactggggtt
    16501 ccatatgaca gcacaactgg taaaacgctt tgttatgctc tttctccttc aggtgcgcca
    16561 gctcgtgcac cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga
    16621 cgcggatctc tgcttttgct ttcagcttgc cgccctgcac acgcgaaaca gcggtatgta
    16681 acccgagcgc gtttttcagc acatggatct tattgtcata catcacttta ttgatcggcg
    16741 gggcattacg cagaaactga tttttcagat cctgagtata ttgccagagg gctttatcgg
    16801 tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt
    16861 taatcagtgt tcgcacctgg gaaagtagct gctcgggata accctgaaga taagtaagat
    16921 tgctcataac cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc
    16981 aatgtcgaaa ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc
    17041 actgacacta caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc
    17101 ggcggatgaa tatttgctgc aacagttgga cgacacagaa atccgcggcc cggtgttgat
    17161 cctgaatgat gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat
    17221 tggcgactca tacatcagtg aactggcgac gcgcgagaat ttacgcctca acgggattga
    17281 tgagtcgagc gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt
    17341 gctgatcaaa gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa
    17401 agtggtcacg tcggatacac gtattattgc cggtgccaag gcccgtgaca ttcacacttc
    17461 cacgctggaa ctgttcgaaa aagtgctcgg cccgaccacc accacgctgg catggaaaaa
    17521 agcgcgcctg attaattgca ctttcaatga accgccgctg gccgatgcgc cgcagaccgt
    17581 tagctggaag ctggaaggta ctgactggac tatccacaac catgcgaatg tcttctcccg
    17641 caccgggctg gatattggcg cgcgcttctt tatacagcat ctgccagaga atctcgaagg
    17701 tgagattgtc gatctcggtt gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa
    17761 tccgcaggcg aaagtggtgt ttgtcgatga atcaccgatg gcggttgctt ccagccgttt
    17821 gaacgttgaa accaacatgc cagaggcgtt ggatcgctgc gaatttatga tcaacaacgc
    17881 gctctccggc gtggagcctt tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca
    17941 gcaacatgcg ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct
    18001 gaaaatcaac ggcgagctgt atatcgttgc caaccgtcac ctggattact tccataaact
    18061 gaagaaaatt ttcggcaact gcaccaccat cgccacgaat aataaatttg tggtgctgaa
    18121 agcagtgaag ctggggcgtc gtcggtaaga ttgacagcta cctgccagat aaggcgtaaa
    18181 cgccttatct ggcctacgtt cagcacgtta cccgtaggcc gaataatatg cgtcgcatcc
    18241 ggcgcttcaa ataacgattt aaatctccag cgcaagccgt gtcccctggg caattgcccg
    18301 tcgtgcgtcc agctccatag ctacatcgca gccgccaatt aaatgcacag ttttcccgct
    18361 atcaatcagc ggttgcgcca atgcgcggtt tggctcttgc cctgcgcaga tcaccacatt
    18421 gtccactgct aatacctgcg gttcgccgtt gatcaccaca tgcagcccgt catcgtcgat
    18481 cttctgataa cttacacttg ggatcatttt cacgccgcgc gagagcaggg tggtgcgatg
    18541 gatccagccg gtggttttgc ctaacccctg tcccggtttg ctggctttgc gctggagcat
    18601 cacgatctgc cgtgggctac gagggatctg cattccctgc gggcttaagc caccagcctg
    18661 ttgtaggcta ctgtcgatcc cccactcatt acagaactcg gcgatattct ggctggtgga
    18721 ttcgcccggc tgacttaaat acatcgccgt atcaaaacca atcccgccac aaccgatgat
    18781 ggcaactttg ttgcccaccg gcgctttgtc gcgcagtaca tcgagataac tcaatacctt
    18841 cggatgatcg attccgtcga taggcggaac gcgcggcacg atcccactgg cgaggatcgt
    18901 ttcatcgaac gcctgtaact gatccgccgt cacggtgtga ttgagtttta gcgtcacgcc
    18961 cgttacttcg atcatccggc ggtagtagcg cagcgtttcg taaaactcct ctttgccggg
    19021 gatctgtttg gcgatattaa actgcccgcc gatctcactg tgcgcgtcaa acaacgttac
    19081 ctgatgacca cgcgccgccg cgttgatcgc aaacgccagc ccagctggtc ccgcaccgac
    19141 caccgccaga ttttttttct gcacggcggg aaggattagc attttggttt cgtggcaggc
    19201 gcgaggattc accaggcacg aggtgacctt gccaacgaag atctgatcga ggcaggcctg
    19261 gttgcagcca atgcaggtgt tgatctcatc ggctcgccct gattgcgctt ttgacagcag
    19321 ttccgcatcc gcaagaaacg gtcgcgccat cgataccata tcggcatcgc cgcgcgagag
    19381 aatatcgtcg gcaacctgcg gatcgttaat ccggttggtg gtcaccagcg gcagcgagac
    19441 gtgacctttc agtttgcgcg tgacccagct aaatgcgccg cgcggcacgg gcgtggcaat
    19501 ggtcggaata cgtgcttcat gccagccaat gccggtgttg ataatggtcg cgcccgccgc
    19561 ttcaatggcc agcgccagct ctaccgtttc ggcaaaagtc ccgccgcctt cgaccaggtc
    19621 gagcatcgac agtcggtaga taataatgaa gtcgttgcca acgcgttcgc gtaccgcact
    19681 caccacttca acggcaaacc gcatccggtt gtggtaatcg ccgccccact ggtcactgcg
    19741 ctgattggtg cgcagcgtca gaaattcgtt gatcaaatac ccttcggaac ccatcacctc
    19801 tacaccgtcg tatcctgcct cccgtgccag ttgcgcgcag tgggcgaaat cgtcgatcag
    19861 ttgcaggatc tcttcatggg tgagttcgtg aggaacgaaa cggttgatgg gggcctgcaa
    19921 tgcggaggga gcgaccagat gcggttggta gctgtagcgc ccggtatgca aaatttgcag
    19981 ggctattttg ccgccttcct gatgtacggc ttcggtaatg gtgcgatggt gtgggatctg
    20041 gctggcgtca ttgagcattg cgccaccttc cataccaacg cctgttaaat ctggtgcaat
    20101 gccgccgctg acaatcagcg ctacgccgtg acgggcgcgt tcggcataaa acgccgccag
    20161 ccgttcagca ccgtccgggt attcctccag cccggtgtgc attgaaccca tcaacacgcg
    20221 gttttttaac gtggtaaaac ctaaatccag cggggcgaac agcgacgggt agctcataaa
    20281 attgtccagt atgtaaaata attgttatgt ggtcggatga gttctaattt agccgtcgcc
    20341 ggagaaaagg gaaaagggga gtgcggggtt tgtgatggga ttcaaaaaga atacgcccgg
    20401 tggtaacgcc ggatgcgacg cttgccgggc cctatccgac ctgggggatt gcacatgtag
    20461 ggcggataag gcgttcacgc cgcatccgcc agtggcgcgg tgcagacgcc ggatgcgacg
    20521 cttgtcgggt cttatccagc ctacgggaac gaacatgtag ggcggataag gcgttcacgc
    20581 cgcatccgcc agtggcgagg tgcagatgcc ggatgcgacg cttgtcgcgt cttatccagc
    20641 ctacgggaac gaacatgtag ggcggataag gcgttcacgc cgcatccgcc ggtagcgagg
    20701 tgcagatgcc ggatgcgacg cttgtcgcgt cttatccagc ctacgggaac gaatatgtag
    20761 ggcggataag gcgttcacgc cgcatccgcc agccgttgca aattattgct tacggaaaaa
    20821 atcgttatac aacataaaca aatgcgcggc actccaggag aaatttggtg cgccttgctg
    20881 cgcgccagtc agcgggttgt aattctcctg aattgggcca tcggcggtta atcctttggc
    20941 atgccggaag aacgtatccg ccagcttcag ggcatcatca cgataaccgt aacgctccat
    21001 ccctttcaga ccaaaccaga actgatccac ccatacgcgc ccgcgccagt agatatcagc
    21061 gccaaaggcc gggttggtta acgctgccgt tcccagcggg acaaaggtgt tgaactcttt
    21121 aggatcgagc atcaccttca ccaccgcgtc ggcattggcc tgcgttgccg caccgttaaa
    21181 cagcggtgac cagccttccg gccctttacc gcgctcaaca atcggtttgc ccgcgcagcc
    21241 gttcgccagc ggtttatctt caatacgcac gtcatagaag tactgcgtag ccgggtcgaa
    21301 catacaggtg ttgatgtagt ccgcgagctg ctgtgccaac tggcgatagc gcttagcctc
    21361 ttccggttta ccgagaatcg tcgccatctc cgccagataa tggttatcgc tgtacatata
    21421 gctggcctga tccaccgact cctgcagtag cgagtagccc agcaacgttc cgtcctgact
    21481 gcggttttcg gcgaatttca ccgtccagtc gctacgtttg ccgccgcttg caacatattt
    21541 attcagctgt tctttgtcga taaacccaaa gacggcggcg tcatcacggc ccgattccca
    21601 cgatgcagca acctgtgccg gaatttccag actgtcgtac tggcctttct ccaccacgcg
    21661 ggcgtagttg ttcagcccag actgcgtctc ttctttgttg cctttcttca ccgtaaacag
    21721 catctcgccg ctctcagtgt tgtgggcttt gtcgcgggtc gcgccatatt ccggcacgcc
    21781 gttgccgttg tgatcgcggt tacgtaacca ccagtcgtga taggccacca gtttcgggta
    21841 catctctgcc agccaggttt tatcctgggt gacgttatac acttccatca ccgaccaggc
    21901 ggcaaggctg ggtttggtat tacgttcgtt ccagttgccg ccatcgccac cacgctcggg
    21961 gctaaggttc cacgctatca ggtcggggac aaagcccaca tcctgcggac gcacgcggtc
    22021 gcctggctgg atctgccagg agaagaccgc gcggatattc tctttggcga tgtccggatt
    22081 gaaatgcgcc atcgcaaacg cctgcttcca ggtatcccac ggccaggtct gattgccgga
    22141 gaaccagcgc ccggtcaccg acggtgtgac ggtgttaaat ttcaccgccc cgccaggcga
    22201 acgccagtta ccgttgagcg tttcgatcgc tttcaccgcg acgcgcgtct gttccggcgt
    22261 cgcatccgga ttggttaacc ctttcttcag atactcttcc cagcgttgct gcgaggcggt
    22321 gagataaaac gccggacgcg ccagaatatc gcggatctgc atttgctctt tgctaacttc
    22381 ctgagcggtc agcagatggg aataggtggt gtaaagtgtg gtcgaaccgt tgatatgcgc
    22441 cttactggta aagcgattac cgttgatttc agtctgcacc ggcagggatt tatgcacctg
    22501 atattctgat tcaccggagg tcagcagatc ccaggtggcg cgcactttgc caaaggtgac
    22561 tttcaggcca tcacgggtgg cgctgatttt gcgctggtag tcagggtatt cgccagcaat
    22621 ggttttatcg gaaagcggtt tcccttcttt cgcttccagt ttttccagca gttcgccatc
    22681 ccacaccaga tacagcggtt tattgctgat gattttagtt tccagtagtg acgtgcgcgg
    22741 cgtggcgaag cgcagagtca tttcgacctg cacatctttt gctgtcagtt tttgcaccag
    22801 cgcgccggga atactgtatg cctccagcgt gaagtcgact ttcttgccgt cctgccagac
    22861 ggtcagtcgg tcgaaattgc tggctataaa gttgatgtac tcttccgtca gcagcgcaac
    22921 gcctggaaaa ccgcccatgg tgttagggcc gtctggcaac agatgaccat gccaggctcc
    22981 gagatcgaaa aacggattaa aacgctggtg atcgtcgtaa tcgtaatcct tcatgtactg
    23041 cggcgcacca gtacggttaa tcacgttttt ataattgtcg gcgttagcgg catgggcgga
    23101 aaaacttatc agcagagcgc aggttactgg cgttaaaata gttttgattt tcatgaaatt
    23161 acagtcctta ttaccagtag aaatattgca tcaggaagat ttggtcgctg ggatcgttag
    23221 aggcattttt catgtagaag cggctatcaa aagccgttgc gaaacgtccg ccgccatatt
    23281 cgtaccagat accaaactgc atgaaagagg tggtttcatc gtcagagtgt tcagggcgat
    23341 gtttggcgta ggtgtaagcg gtggagagat aaacgccctt taacgcttca tacatggcgc
    23401 tcaccatcat cccgctttca cttcccggta cgtccagccc gtcggcacgt ccggtatggt
    23461 gccaggcgcg agcggcaaag ttaggggtaa gcaggcccgt gagatagagt tgtcctgtgc
    23521 cgtcggtaat ttccagaccg ttcatccagg tgatgccttt ggcaaggtca tactggatgt
    23581 aaccgttcat cattgccgga taggtgtatt tgtcgtcgta gcgatcgtac ttgccaaaat
    23641 gtagccatgc tttgctttcg tcatgatgtc cggcaggtgt ggcggtgacg ctataacgaa
    23701 agttgccgct taagttctgc accttcagcg cggtcataat gtcgcgggta ttgggaatga
    23761 cgtagcccaa atcgggtgtg aagtcgcccc accattgcag gtcatccagc gaggaatcct
    23821 gacgcagact gagcatgatt tcggtgccat cattggtacg ataaccgcca tagaaacggt
    23881 taatcccacc ttcgaaacca ccccagctat ggtcaggcac ccaggcatta ccgtcgtggt
    23941 cggcctgcac ggtccagcct tcgccgtaaa tcaggccgaa ccaggagccg tacttcatct
    24001 ccaggccacc ttcaatatag gtgccgtcgc tgtatttatg tttattctca ccctctaaat
    24061 ccatatatcc accaacccct aattcgccgt aaaaacgaaa tgaaggagaa gatgtcgagg
    24121 taattgcggg tgtttttgtg tcgtcatgtt ctgccggacg tgcctcttct cctgccgcag
    24181 aaaaggcgta gcaaaacggc agagcaagta atgctctgca tggtgcagtg attaatttca
    24241 ttgtgttaat ccatgagcta aagaaaaagt acagcctttt atcgttgtaa ttataaaaat
    24301 ataaaggcag aaactatttt ccctctcctc atccaggaag agggaaaatg cctgctcatt
    24361 ttttattatt ctgcttaatc aacattgctg gctggcgtcg cttcaatatg gcgctcttca
    24421 gccgttaatc cctttatata tttactgtat ttccaccacg caaagccaag gaagataaca
    24481 ataccgccga cgttataaaa aatgatggtc agaatattcg cgccagtcgg gaatgtcgaa
    24541 gcgacaaacc ctacggcaaa tatcgcaatc agcattgaaa caacgataat accggtgcgg
    24601 cgggagccca tacggaaatc gcgtggcagg tgatctaatt tggcgcgtaa attcaggtaa
    24661 gccagcatga tgaataacgg cggaagcatg gacgctgcgg cggtcatatt aataatagta
    24721 ttcatcagat cctgcacggt attggaaccg agcatcggga taatcatcag cgggatgacg
    24781 atcaggaact ggatccacgc tgcgcgcgcc ggaacgccgt tttcgttaag ttcgacggtt
    24841 ttcttaccaa agatgccttc cgggatttcg gagaagaaaa ttttcaccgg cgttgcggtc
    24901 cacatcagca gggaaccgaa cattgcggta aaggacacca gaccgacaaa gcgattcatc
    24961 aacgcttccg gcagaccaaa atacgccgcc atgccgtgga atacctgcac cgatccgccg
    25021 gtaaatttca actctttgct gctgacgaag acgttaatca gcaccgagga gacggaatac
    25081 agtacgccga taaaaatccc ggcgaggatg atcactttaa cgaacgattt cgaaccgcct
    25141 ttgacgtcgt taacgtacac cgcgacggac tccgcgccac ctgcggccat aaagatccag
    25201 gtggtaacgc cgaggaacgc ccagttgaag ttcgggatca tcgcgtcaac ggtgatcgga
    25261 tcggcaggct gtacgccgcc aaccagcgcc gtacctgcca gtaaaatgta ggagagcgtt
    25321 aacagcagca tcagcgttga agtgacggag gtaattggcc ccaacatttt cgccccgttg
    25381 gtggaaaccc aggtggagaa ggcgaacagc accatactga taatggtggt ggcaaccggc
    25441 gtcataatat attcgtatcc gaggaaggca tacgaagcat aagcaataac gcgcggcaac
    25501 agcgaggtga aaaagaacag gtttacgaac cagtaggtat aggcagtaat aaatgcccaa
    25561 cgtccgccca gcgaactttt tacccacgcg tagacaccgg cttctgagtt tttatttaac
    25621 gaaacaaatt ctgcgatgat cagacagaag ggaataaaat aaaaaatcgt cgcgaggaaa
    25681 aacatcggtg ccgaggccag tccaagctca atattattat tgataacgtt gttaaagctg
    25741 taaacggcgg caaaagtcag cgagagcaag ccgaatttgc cgattgtatt acgtttggta
    25801 tcagacataa cacatctccg aaaatgagta gggtagcggt ggcggcaaac tccttttgcc
    25861 gccgtagttg ttatttgtta tgaaaataac catcttcgat ggtgactttg agaaccactt
    25921 ttttgaccgc gttattgcag ataaaccgat atgcttcatg gatatcgcaa ataacgattt
    25981 gcccttcgtg gacctcaacg gtttctccgc agccttttaa atattcacgg tcagtttcat
    26041 cacgataata ttccacgacc tgtaatgtct ctttcggcgc atattcaatt ttttgctgcc
    26101 cttgcaggta gtaatgcact tcaaaatagc gtcgatgccc ggtaaatagc gcatcggtcg
    26161 cggagtctgt ctccacgcgg taagtcaatg agtcaccgat ggagtgggcg acgccaggct
    26221 gaatgttgtc gatattttca atcgcttcaa cgcagcgttg ccacttctta ccagaggcgt
    26281 aaatctggcg gaactgttct aagttatcga tgatcctcat tgcttattct cgctgtgcaa
    26341 attcgtggaa aagaaccctg cgccgaactc atacgacgcc aggctttgcg cggtagcttc
    26401 tccgccagaa accggcagca acgtaaagcc gtagctgaag tcacggaacc agacgcgcca
    26461 ggagtccagc acctcgctgc cccaggagtt ggagccgagg ccaagcagct ggtggtcgag
    26521 attcagggtg atgtcatcac tgcgctgcag ctcgttacag tgctgggcag cgtggatgtt
    26581 ttcctgggta tagtgccagg cgctgaagtt aattgggcgc tgcggaacca ccagcagacc
    26641 gttgccgtgg cggttagtca gtgccgtcca gcggacatgc tgacggttgc cgttgttctg
    26701 cgggaaggga tagttctcga acatggcatc gacggtgctg cgccagatat cgatgatgtt
    26761 agcctgctgg ctgtcggcgt agttttcgcc cggtccacga ccgtaatacg ccacctgatc
    26821 gtattcgccg ttaatcccca tggtgaaacc gatgcacgga atgatgtgcg gatagtcgcc
    26881 gtaacgctcg ccggaaagcg ccacgttaac ctggccatct gcagcgatgc gccagatgta
    26941 ggtgcagcgc atcccgaagt caaacaccgg cggtgctata accgtgcggc tgatgatcaa
    27001 cacttcacca tcgctctgtt ctacggcaaa gtcgcgcaga tgttcctgca tgatctgcaa
    27061 atgattcggt tgccacagcc cttcgtactc ctgcttgtgg ttgtcgatca ttggcttgaa
    27121 gaagttgatc tttggctcgc gggtcagcag cgactcgcca ttcacctgcc aggatctcgg
    27181 tttgccactc atttttgaga aggtgatcgc gaagttgtag ccgcgaacgg tgcagctcaa
    27241 acgatcgtct tccagcgtca gcggacgcgc attatttggt gcgaaaggca ctggctgcgc
    27301 ggtgttttcc ttcagcggga actgataagt ggcgatagaa tgcccggctt cgctgtagcg
    27361 ggtgcgggaa tctttggtca ccgtaatgtt gaggaacgct tcgcgggcgt ccagctgcgg
    27421 cagcgtgatc tgcaaggggg cttcgctgtt cggcgcaacg tcgcgcagtt taatctgttg
    27481 cgtcgcgagc gtttcacctt cggcgcgcac ctctgcgtgc agggtgtagt catccagcgt
    27541 ggtaaaccac agtttatttt cgactttcag ctcgccgcga gtcagatcca gcgcgtggat
    27601 ttttaccggc gcgataacct gtttgtactc tttcaggcct ggtcccggcg tctgatcgga
    27661 atagatcaaa ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc
    27721 gaatttatac cagacattgc cgttgtcatc ctgcgcctgg atcccgtggt cgcaccactc
    27781 ccaaacataa tgtccctgaa tacagtcgtg cttatagaag acgttctggt actccgtcag
    27841 cccgcccggt ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg
    27901 cgggtattca ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc
    27961 gaccacttca gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcctt
    28021 cgctgcgtgg tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca
    28081 gatgatgatc gacggatggt ttttctgcgc gtgaatatgg cgaacaatgc gctcgacgta
    28141 gactttttcc cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc
    28201 gtgcgattcg acgtcggttt ccgccatcac aaacaagccg tagatatcac acagttcgta
    28261 aaaacgcgga tcgttcgggt agtgagcggt acgcacggag ttgatgttgt gctgcttcat
    28321 caactggaga tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt
    28381 gtcgtgacgg ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc
    28441 gcgcactttg atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtc
    28501 ggcgtctttc agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg
    28561 gggctgttcg acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc
    28621 aatggtgctg ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtaac
    28681 gacaggggag gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata
    28741 ggcttcgtca aagtcggtac gcacggtgaa atcgttaata tgcgttaggt gttttccgac
    28801 cagataaaca tcgcggaaga tccccgccga ccaccacata tcctggtctt ccacgtaggt
    28861 ggagtccgcc cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc
    28921 gctgatgtca aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt
    28981 aacgtagact tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg
    29041 ccagccgtcg ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac
    29101 aaacggcaca tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc
    29161 ttccatttgc cacatggcgg ggacggtaat gtgtccccag ccagccatta actccgaggt
    29221 gaaggcttca ggcacttgca gcgggtggtc aaaaaaatgg aaattccact gaccgcttaa
    29281 gggcagaaac aggctgctcg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata
    29341 tgaaaaaaag tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca
    29401 gcgattcata gggcctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg
    29461 tgtaattgct ggggagttta gtaaaatttt tactaaaaag tagcaggtaa atgcatttac
    29521 tttaactgga tcacgaaaat aaacggcagc acagtccggc aaaaacgact gccggactgt
    29581 gtgtaagggg atttaacggg tcgtgccgcg cagttttaat ttgctgggaa cgaagactaa
    29641 cagcggcagc gcgcgaccat cgcgggcttt ttcatacacc aggttaacgc cctgacttcc
    29701 catcatttcg gaatggatgc gcacggtgga gagcggcgga aaggtaaatc gcgcggtggg
    29761 gatatcatta acgctgataa gcgaaatatc ctgtgggatg ttcagaccac gttcatgaat
    29821 cgcccgcagt acgccgatag caatggaatc ggaagcaaca aacagtgcct tcggatagtc
    29881 ttcccgcgcc agcatctgtt ttgccagttc ataacccgac gaactggaaa aaccgccgcg
    29941 ccagatatcc tcttcgcgta ctacttgttt cagacggcca tattccgcaa aggcgacctc
    30001 acgaatatcc gccttgccag gctcatcttc accgccaata aaaccaatgc gattaacgcc
    30061 ctggttgata tagaagtcga tgatttcttt actgatgcgt gccagatcga tatccaccgc
    30121 atcgtaaccg ctgccgggtt cgtgaaagtc gataaaacag atattgtcgg tcaacgcgct
    30181 ggcagcggcg cgcagggcgg gcgtgggttt gccgacaatt aaaataccgg tgacgttttt
    30241 aatgtctggt aatccgttgt gttcataaca gttggtcagt tcgatcgcca gcttttcgca
    30301 ctgagtttca atgccgtggc ggatcgccag atagtaagga tcgttgatct ccagctcctg
    30361 ctggtagctg tagatagcca gaatatggtg ttggttgact gcgcctgtct gtagtttacg
    30421 ggcgctactg gtcttgtact ccagcttttc ggcgatctcg agaatgcgat gtttcgtctc
    30481 ttctttcaca ttcaatgtcg gatcgtcatt taagaccctg gatactgtcg ccagggatac
    30541 gccagcttcg attgcgatgt cttttagtgt tgccatgttt acctttttta ctttccctga
    30601 tgtgcgcccc cattgtagag gaagtactta tcgggtcacc tgttttcagt aaataaagcg
    30661 cgtttctttg acgctgtaac ggtaacacag cggctcacga atgtgatcac gcctgcattc
    30721 tccattttta gcctacagga ttgagaaaaa cttttgctat agtttagtaa aattttactc
    30781 gaagggagga agcaatggaa accgtggctt acgctgattt tgcacgtctg gaaatgcgcg
    30841 tcggaaagat tgtggaagtg aaacgccatg aaaacgccga caagctgtac atcgtacagg
    30901 ttgatgtggg ggaaaaaaca ctgcaaaccg tgaccagcct ggtgccgtac tacagcgaag
    30961 aagagttgat gggaaaaacg gtggtggtct tatgcaatct gcaaaaagcg aaaatgcgtg
    31021 gtgaaacgtc ggaatgcatg ctgttgtgtg cggaaaccga tgacggcagt gaaagcgtgc
    31081 tgttaacgcc ggagcggatg atgcctgcgg gcgtacgtat cgtataaaaa gatcggatgg
    31141 cgacgtcgta tcgccatccg atttgatact acgcttcttc gacacttact cgcatggccg
    31201 ccagcgcctt acgcgccgct ttgatcacca gttcacactg ttcaatggtc agtgtcagtg
    31261 gcggttcaat gcggatcgtt ttggcgttat taagcgttcc ggccaccagt acgcgctggc
    31321 ggaacatctc gctggcaaag ttatagccga tttcgttatc aacaaactca atcgccatca
    31381 acatcccttt accacgtgct tcctgcacca gatcgggata ttcccgcgcc agttgacgga
    31441 aaccgtccag caacatatcg cctttttgtt cagcctgagc cggtaagttc tgctccaaca
    31501 acacattgat ggtcgccagc gccgccgcac aggccagcgg gttgccgcca aaggtggtgg
    31561 tatgcaggaa cgggttgtcg aacagaactg agaacacctc ttcagtggcg atggttgcgc
    31621 caatcggcat cacaccgccg ccgagggctt ttgccaggca caggatgtcc ggctgtacgt
    31681 tctcatgttc gcaggcgaac atcttgcccg tgcgccccat gcccgtttgt acttcatcga
    31741 ggatcatcag tgcgccgaac tcatcgcaca gcttacgcac ggcgatgaga tagcccggcg
    31801 gcggcagaat tacgccacct tcaccctgaa tcggttcgag gatcaccgca gccacatcat
    31861 caccggtttt tttgcactcg ttaagagccg tgcgcatggc ttcgatattg ccaaacggca
    31921 catgacggaa ccctggcagc agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg
    31981 ccgacagcgc gccaagtgat ttaccgtgga acgcgccgct ggtggcaata aaagtaaatt
    32041 tgccgcgcgg cgactgataa gccttcgcca gcttcagcgc cgcttcaacg gactcggtgc
    32101 cgctattaca gaagaagctg tatttcagtt tacctggcgt tagcgcagca agggttttcg
    32161 ccagcatagc ccgtaacgga tcgagtagct cctggctgtg cagtggttgt ttcgcaagtt
    32221 gattctgtac ggcggaaacc acaactggat tacggtgccc cacgttgaaa attccaaaac
    32281 ctcccaggca gtcgataaac tcctgtccct gggtgtcgac aagcgtattt aaacctcccg
    32341 cttgccactc tacggctccg taatccccgc cggcggtaac agatttgcga tactctaaaa
    32401 accccggatt gacatgctct ttgaagtatt caatcacctc tcggttaagt gctttcatct
    32461 cctcatgatc cagcgttcgc ttttcaatga gattcagggc gtgggcgctg cacgctaaag
    32521 ccgatgcgct cgaaggtaac ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat
    32581 accgatttaa gtattgcagg gattgcgcca ctccggctgc gatcgcgaaa atcgggataa
    32641 acacaaatgc ggttatcatt tgcacaatat ttaatgctat ttgttaacat tcgttgttat
    32701 tttgcacggt ttttgtcggg tatggatttg ataattgctg cgcaaatgca cgttttcagg
    32761 gcaataattg cctgattttt gggcaagata attaacgttt tcgtatagta acagagtgaa
    32821 agtattttaa atcaacgaat tagatttgtg ctttaatcgc aaattgcgat ctaaatcaaa
    32881 ttaatcggtt aaagataacc gcagcggggc cgacataaac tctgacaaga agttaacaac
    32941 catataacct gcacaggacg cgaacatgtc ttctcatccg tatgtcaccc agcaaaatac
    33001 cccgctggcg gacgatacca ctctgatgtc cactaccgat ctgcaaagct atatcactca
    33061 tgctaatgac acttttgtgc aggtgagcgg ctttaccttg caagagttac aagggcagcc
    33121 gcacaacatg gtgcgtcacc cggatatgcc aaaagcggcg tttgcggata tgtggttcac
    33181 cctgaaaaaa ggggagccct ggagcggcat cgtgaaaaat cgccgcaaaa atggtgacca
    33241 ttattgggtg cgggccaatg cggtaccgat ggtgcgcgag ggaaaaatca gtggctatat
    33301 gtcgattcgt acccgggcga cggatgaaga gatcgcggcg gtggagccgc tgtacaaagc
    33361 gctgaacgcc ggacgtaccg gtaagcgtat tcataaaggc ctggtggtgc gtaaaggctg
    33421 gctgggtaaa ctgccttcat taccgcttcg ctggcgggcg cgtggagtga tgaccctgat
    33481 gtttatcttg ctggcggcca tgctttggtt tgttgctgcc ccggtggtga cgtatatcct
    33541 ctgtgcgtta gtggtattgt tggcaagcgc ctgttttgaa tggcagattg tgcgcccgat
    33601 agaaaatgtt gcccgtcagg cactgaaggt ggcgaccgga gaacgtaata gtgttgagca
    33661 tctgaatcgc agcgatgagc tggggctgac attacgcgcg gtagggcagc ttggcctgat
    33721 gtgccgttgg ctaattaacg atgtctcaag ccaggtgtcc agtgtcagaa atggcagtga
    33781 gacgctagcg aaaggcaccg atgaactgaa cgaacatacc cagcagacag ttgataacgt
    33841 tcagcaaacg gtggcgacca tgaaccaaat ggcggcgtcg gtgaaacaga actctgccac
    33901 ggcgtcggct gccgataaac tgtcaatcac tgccagtaat gcggcagtgc agggcgggga
    33961 ggcgatgacc acggtgatca agactatgga cgatatcgcc gacagtaccc agcgcattgg
    34021 caccattact tcgctgatta acgatattgc gtttcagacc aatattctgg ccctgaatgc
    34081 ggcggtggaa gcggcgcgag ccggcgaaca gggcaaaggt tttgcagtgg tggcggggga
    34141 agtgcgtcat ttagccagcc gcagcgccaa tgctgccaac gatattcgca agctgattga
    34201 tgccagtgct gataaggtgc aatccggttc gcagcaggta cacgccgccg gacgtacgat
    34261 ggaagatatt gtggcacagg tgaaaaacgt cacccagttg attgcccaga ttagccattc
    34321 aacgctggaa caggccgatg gtctttccag cctgacccgt gcagtggatg agcttaacct
    34381 catcacccag aaaaatgccg agctggtgga agagagtgcg caggtgtcgg cgatggtgaa
    34441 acaccgcgcc agccgactgg aagacgcggt gacggtgctg cattaatcgg tattccggat
    34501 ccggcggttg gagcacaatg cctgatgcga tgctggcgca tcttatcagg cctacggggt
    34561 atgtagggta cgccgcatcc ggcaatcggt gattaatcca gttgcgtaat atcaaaagcg
    34621 gcgcggtcga tcaccgcaat gatctttttt atttgtgcat cagtgatatc gctctggttg
    34681 acgcgtaaat ccagcaccgc tttaaaatta tccagcgccc gcttcatttg cgggttctgg
    34741 cgcagcgcag cgccaacgca acgcgctttg atgcgttctt caatcatctc cacctgttcg
    34801 cggttttctt ccagccactg cgcgccttgt tctgtcagtg caatctgctt cttaccacct
    34861 tcctcttcgc ggatggtaat cagcgactgc tcctgcagaa aatccagcgt cgggtagatg
    34921 acgcccgggc ttggggtgta attcccctgg gttagattct caatcgcttt aatcaattcg
    34981 taaccgtggc tgtcatcgcg cgagagaata tccagaatca ccagacgcaa ttcaccgtga
    35041 ccaaagaaac gttgccgacg accgccgccg cgaccgtggc gaccaccgca acattgaccg
    35101 tgttcatgct ggtgaccgtg tccgcagtgc tcgtgttctg acttctcacc tttgcagcag
    35161 ccctcatggc gtggctggcc ttcatgttta caacaccctt cgtgatgatg gctcatatca
    35221 gcctcctttt gcttaattag atatatctaa tttatatctg atatgagtac tcttgcaaga
    35281 ataaaaatga tttttaaatg tggactcatc tcacacgtag gccggataag gcgttcacgc
    35341 cgcatccggc gttgagtaat gatgcctgag aagaggcggt tcgtcttatc agactataga
    35401 tatatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa
    35461 aattatttag atatatctga ttaccatcac gaaggcgata acaatgaata actccccccg
    35521 ctacccgcag cgcgttcgca atgatctgcg gttccgtgaa ctgactgtgc tacgcgttga
    35581 gcgtatcagc gccggttttc agcgcattgt ccttggcggc gaggcgctgg acggttttac
    35641 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    35701 gccgccaacg gtaacggaag agggcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    35761 tgactatacg ccactgtatg acgaactacg ccatgaacta gcgattgatt tctttattca
    35821 cgaagacggc gtcgccagta gctgggcaat gcaggcccaa ccgggcgata aactcacggt
    35881 ggcaggtccg cgcggttcgc tggtggtgcc ggaagattac gcgtatcagc tgtatgtctg
    35941 cgatgaatcc ggaatgcccg cactgcgccg ccgcctggaa acgttgagca aacttgccgt
    36001 taaaccgcaa gttagtgcgc tggttagcgt gcgggataac gcctgtcagg attatctcgc
    36061 gcaccttgat ggttttaata tcgaatggct ggcacatgat gagcaggcgg tagatgcgcg
    36121 tctggcgcag atgcaaatcc ctgccgatga ttacttcatc tggataaccg gcgaaggtaa
    36181 agtcgttaag aatttaagcc gccgctttga agcggaacag tatgacccac agcgggtacg
    36241 tgcagcggct tactggcacg taaagtaact gatatataaa aagaaaaagg ctgacgattt
    36301 ctcgtcagcc tttgctatat ctggtggccc ctgctggact tgaaccagcg accaagcgat
    36361 tatgagtcgc ctgctctaac cactgagcta aggggccatg gtagcggatt ataaagtaac
    36421 tccgtgtcgc aatccagcca ttaccgcgcg cctgctgttt ttataaacaa tgtattttca
    36481 atccgttata cttttcctgg tgatgttaga aaggagtaaa tatggttgag gatattttgg
    36541 ctccagggtt acgggtcgtg ttttgcggta tcaaccctgg gctttcatcc gccgggactg
    36601 gttttccctt tgctcatccg gcaaatcgct tctggaaggt gatatatcag gccgggttta
    36661 ccgaccgtca gttgaagccg caggaggcac agcatctgct ggattatcgt tgtggcgtca
    36721 ccaaactggt agaccgtcca acggtgcaag ccaatgaaat ttcaaagcaa gagctacacg
    36781 caggtgggcg taagctgatt gaaaaaattg aagattatca gccgcaagcg ttggcgattc
    36841 tgggcaaaca agcatatgaa cagggattca gccagcgcgg tgcacagtgg gggaaacaaa
    36901 cgctcagcat tggttcgacg cagatttggg tgctgccaaa tcccagcggt ttaagtcgcg
    36961 tttcactgga gaaactggtt gaagcgtatc gcgagctgga ccaggcgctg gtggtgcgtg
    37021 ggcgataaaa aacgccaccg aaaaggtggc gtttgtgcaa agggacaggt gccgggtgcg
    37081 gcgtaacgcc tgatccagcc taccgattaa tcgtccagga agctacgcag cacttcagaa
    37141 cggctcgggt gacgcagttt gcgcagcgcc ttcgcttcga tctgacggat acgttcacgg
    37201 gtaacgtcga actgtttacc cacttcttcc agcgtgtgat cggtgttcat atcgataccg
    37261 aaacgcatac gcagaacttt cgcttcacgc gcggtcaggc cagccagcac gtcgtgtgtt
    37321 gccgcacgca ggctttcggt ggtcgcagaa tccagcggca gctcgagggt ggtatcctcg
    37381 atgaaatccc ccagatgcga atcttcatca tcaccgatcg gcgtttccat ggagattggc
    37441 tctttggcga tcttcagcac ttttcggatc ttgtcttccg gcatcagcat acgttcagcc
    37501 agttcttccg gcgtcggttc acggcccatc tcttgcagca tctggcgaga aatacggttg
    37561 agtttgttga tagtctcaat catatgcacc ggaatacgga tggtgcgcgc ctgatccgcg
    37621 atagagcggg tgatcgcctg acggatccac caggttgcgt aggtggagaa cttgtaacca
    37681 cggcgatatt cgaatttatc aaccgctttc atcagaccga tgttgccttc ctgaatcagg
    37741 tcaaggaact gcaagccacg gttggtgtat ttcttggcga tagaaataac cagacgtaag
    37801 ttcgcttcaa ccatctcttt cttcgcacgg cgggctttcg cttcaccgat ggacatacga
    37861 cggttgatat ctttaacctg ctcgatggtc aggccggttt cttcttcaat ctgctgcagt
    37921 ttctgcaggg cgcgatgcac ttcttcagag acatcgtgca gtttttccga ccacggcttg
    37981 ttcatcgcaa ttgccgcgtt gaaccaggta tcgctggttt cgttgccggt aaacagggta
    38041 atgaagtttt tcttcggcat tttgcactgc tcaacgcaga gcttcatgat cagacgttct
    38101 tgcgtacgaa cgcggtccat catgacgcgc atgctgttga ccaggtagtc aaactgcttc
    38161 ggcaccaggc ggaactgttt gaatacttca gacagtttca ggatctcttc ctgagcagca
    38221 gcgtgactgc gacctttcgc tttgatggtg tcacgcgtta caacgtactg agcgcgcagt
    38281 tccgcaaatt tttcgcgagc cagttccggg tcgatgctgt tgtcatcatc ggcgctgtcg
    38341 tcatcgccat cttcttcgtc ttcatcttcg tcatcgtcca gatcttcctg ggaaagctca
    38401 gaaccgacgt gagtggcggt aggtgccaga tcttcttctg cgttcgggtc aacaaagccg
    38461 gtgatcagat cggacagacg cgcttcttct gcttcaacac gatcgtactg ttccagcaga
    38521 taggtgatcg cttccggata ttcagcaacg gagcattgaa cctggttgat cccgtcttca
    38581 atacgcttag cgatgtcaat ttcgccttcg cgggtcaaca gttcaacggt gcccatttca
    38641 cgcatgtaca tgcgtaccgg gtcagtcgtg cgcccgattt cagattccac gctggaaagc
    38701 acctgcgcgg cggcttcggc agcatcttcg tccgcggtgt tttcagccag catcagatca
    38761 tcggcatccg gtgcttcttc catcacctga atgcccatgt cgttgatcat ttggatgatg
    38821 tcttcgatct gatctgaatc gacgatatct tccggcagat ggtcattgac ctcggcatag
    38881 gtcagatagc cttgctcctt accacgggtg acaagaagtt tcagctgtga ctgcgggttt
    38941 tgctccataa gacggtatcc acacttattt catgaggttg gtgttgggcg attgacccga
    39001 tgtccttcag ccgttaacgc tgaaatccaa cggttgttta ccgccaacga taattacgag
    39061 ggcgtactta tatatttgcc gctgcctctc agtgcggctg tcgggggctt cccgatcgct
    39121 cttcggcact taagccgtta aatcactttt tcgccagctc ctggtttaat gtccagagct
    39181 ccaggcgttc ttcgttgctt aaaccatgcg tgcgctcacg agcgattaac tcttcctggc
    39241 gcagttcaag cagcgaatca aacatatggt tgagtgagtc ggtgaaggtt tgctcagcaa
    39301 tattcttatc tgctatatcg tcccacatcg acagtttttc aagggtggca gcattatttg
    39361 taccacgata gtgctctaaa agttgcccgg tggtcagacc tggctgggag agacaagtgt
    39421 tgaccagttc tctgaataag ccaagtccag ggagcttatt ttcatccaga ttctcaagcg
    39481 gcgggaccaa cgtcgctaat tctggatttt gcaccagcaa ccctataagt atacgcatgg
    39541 tcgtgcgttt tagctgcgga acagggcgag aaacgccgct ctctgccgct tttggcatta
    39601 atcgttcaag ctggctgtca tcaagtatgc ctaatttgtt gcctaattcc tgacgaagat
    39661 atattcgcag cgtttcgccc ggcacctgcg atatcaatgg cagtgccagc gtactcaaac
    39721 gtgcgcgccc gtcaggggta ctcagatcaa cttgcggcat caggctgtta aacagaaatg
    39781 cggagagtgg catcgcctgc tccatccgcg cttcaaacgc ttctttacct tcttttcgta
    39841 ccagcgtgtc agggtcttcg ccatcaggca aaaacataaa gcgtagctga cggccgtctg
    39901 tcatgtaagg cagcgccgtt tccagcgctc gccaggcggc atcgcggcct gcacggtcgc
    39961 cgtcataaca gcaaatgaca ttgttggtcg cgcggaacaa cagttgtatg tgatcggcgg
    40021 tggttgacgt acctaacgac gcaacggcgt aattaatgct gtattgcgcc agcgccacca
    40081 cgtccatata gccttcgacc acaagcagac gattgggttc agcgttatcc tgctgcgctt
    40141 cataaagacc gtaaagctgg cggcctttat ggaaaatgtc tgtttccggc gagttcaggt
    40201 atttgggggt atcgttgccc agcacacgcc cgccaaaacc aatcacccga ccgcgtttat
    40261 cgcgaatggg gaacatcacc cgctcgcgga aacgatcgta actgcgtccc tgatcgttag
    40321 tgaccaacat gcccgcatca atcaatgact ggcgattttc tggattgccg ccaaaccgct
    40381 tcaggacgtt gtcccagccg gggggcgcaa aaccaatcgc aaagcgagcg ataacctcgt
    40441 ggcttaatcc gcgtttttcc agatactggc gcgcagacgt ggcaacaggt tgctgtaaag
    40501 attgttggta aaacgtattc agaccgtcca tcaactgata aagcgtttgc cgctgatggc
    40561 gctcgatctg gctggggccg ctgcctgctt caaatggcac ttcaagattg tgcattgctg
    40621 ccagctcttc gaccgtttcg acgaactcga gcttgtcgta gttcatcagg aagtcgatcg
    40681 cgttgccgtg cgcgccacat ccaaagcagt ggtaaaactg tttctcaccg ttaacggtga
    40741 aggacggggt tttctcgttg tggaatggac aacacgcgtg gaaattcttg ccctgctttt
    40801 tcagcttcac acgggcatcg atcagatcga cgatgtcagt gcgtgccagc agatcattaa
    40861 tgaatacgcg tgggattcgt ccagccatag gccccgattt ttagcaattc ataaacgaaa
    40921 ataagccgcg cattcctttc ggaagcacgg ccttacaact acaactcggt ctgatcggag
    40981 agcaatgctc tcggggaatt agtacagacg agtgcggcgt gcgttttcgc gagccagttt
    41041 cttcgcgtga cgtttcactg cagaagcttt agcgcgctta cgttcggtag tcggtttttc
    41101 atagaactca cgacgacgaa cttccgccag aacacctgct ttttcgcagg aacgcttgaa
    41161 gcgacgcaga gctacgtcga acggctcgtt ttcacgtact ttaattaccg gcatgtgcct
    41221 ctcacctttg attaattcgg tttgccgctg gcatcaacgc cagcttattt caaaatggtg
    41281 cggaatttta ctgcaattgc tgctgctttg taaagcaccg cggccttttt tgaaagggac
    41341 ttttataagg gtgaggagta tacacgaggc tttctccagg ggcgaacaaa gttttacatc
    41401 aacccgcatt ggtcctacac tgcgcggtaa taaagcgagg taaaacaagt catgcgtgta
    41461 ctgggtattg aaacttcctg cgatgaaacc ggcatcgcca tttacgacga tgaaaaaggt
    41521 ttgttagcca accaattgta tagtcaggtg aaattgcacg ctgactacgg cggcgtcgtg
    41581 cctgaactgg cctcccgcga tcatgtgcgt aaaaccgtac cgttgatcca ggcggcgcta
    41641 aaggagtctg gtttaacggc aaaagacatt gatgctgtgg cctataccgc aggccctgga
    41701 ttagtcggcg cactgctggt tggcgcgacc gtggggcgtt ctctggcgtt tgcctgggac
    41761 gttccggcga tccctgtaca ccatatggaa gggcatctgt tagcgccgat gctggaagat
    41821 aacccgccgg aatttccgtt tgttgcgctg cttgtttccg gcgggcatac gcagttaatc
    41881 agcgtgactg gcattggtca gtacgagctg ctcggcgagt ctatcgatga tgccgccggg
    41941 gaagcgtttg ataaaaccgc gaagctgctg gggctggatt atcctggcgg gccgttactg
    42001 tcgaaaatgg cggctcaggg tactgccggg cgctttgtct tcccgcgtcc gatgaccgac
    42061 cgtccggggc tggatttcag cttctccggc ctgaaaacct tcgcggcaaa taccattcgt
    42121 gacaacggca ccgacgacca gacgcgtgct gatatcgccc gcgcctttga agatgcggtg
    42181 gtcgatacgc tgatgattaa gtgcaagcgg gcgctggatc agacgggctt taagcgactg
    42241 gtcatggcgg gcggcgtgag tgctaaccgt acgttacggg cgaagctggc tgaaatgatg
    42301 aaaaaacgcc gcggcgaagt gttctacgcg cgtccggaat tttgtactga taacggcgcg
    42361 atgatcgcct atgccggaat ggtgcggttt aaagcaggcg cgacggcgga tctcggcgtt
    42421 agcgtgcgtc cgcgctggcc gctggcggag ttaccggctg cgtaaaacta ttgggtgccg
    42481 gagagcaatt tccggcaccg tcctcactta aagcaacacc acgggcatcc acagtaagcc
    42541 ggtaatcacc agcaacacga ggaatatcag cccaaaaatc gcccccagtc gccagtaatc
    42601 cgccgttggc agataaccac taccgtagta aatcggactg ggtccggtgg cgtatggtgt
    42661 aagaatgctc cccagaccaa ttgccgcgcc aaccatcagg cagaacaccg gcagcgggat
    42721 ttccggcatc gccagcgcgg cggcgatcat catcggtgcg agagcggagg tatacgccgt
    42781 ggcgctggcg aaaaagtagc gcagtagata aaacaccaca atcaacgcca ccatcaccat
    42841 cgttggcgaa taaccgctta agctgcctgc taacagtttg ccaaaccagc taataaaacc
    42901 ggtgttgttg agtccggtcg cgagggttat aagcgaggcc agccagaaga aaacgttcca
    42961 cgccgcttta ttactgacaa tatcgtccca actgataatg cgcagaagca gcatcagcgc
    43021 caccacgctg taaccgacca tcgcggcatc gatataatca ccgccgaaaa tccacagcac
    43081 cagcgcgcct accatcagcc ccagcatccg tttttcacgc gaacagagcg ggcccattgc
    43141 ctgcagttcc ttctctgccc agcgcggcac ctgatcgcct gacttcagta caggcgggta
    43201 cagcacgtaa gccagccagg gaaccagcag gaccagtaaa atactgagcg gcaacatccc
    43261 aaggaaccag tcggcccaac tcagcgtggt gtgagatgcg cttttcatca gtccaattaa
    43321 cagcaagtta ggagccatcg ccgtcaggaa aatggcgctg gtcacgcagt cggcaacaat
    43381 ccccatccac atgatgtacg agccaatact gcgcgaactg ctgtcgtttg gttgtgattg
    43441 atagagcggc ggcaggttac ggatgatggg atagataatc cccgcaccac gcgccgagtt
    43501 ggacggtgtg acaggtgcca ggatcagctc ggagaacatc accgcatagc caagaaacag
    43561 cgtgcgatgc cccatctttt tcaccagaat cagcgcgata cgtcgcccca gcccggtttt
    43621 ttcatagcct gtgccaaaca taaaggcggc gaaaatcagc cagataaccg aattagaaaa
    43681 accggaaacg gcccacgaga gggattttgc agtgaattta aagcctggct gagcgagctg
    43741 ctccgggctg aacagcagcc agggagagag tatggcgatg atggagatac ccaccatcgc
    43801 caccacggca cccggcacgg gttcgaggat cagtccaacg attacgccag taaaaacggc
    43861 aaagtagagc caggtatgac tctccagccc tgcgggaacc ggaattagag caataatggc
    43921 gatgaccgcc agcggcgcaa ggtatcgcca ccattcagtg gaaggtttca tcgtgttatg
    43981 cctgcgaaaa aggagagggg cgagcctctc cgggagggtt atttgatgta atggacgtgc
    44041 tcgcagatct cttccacgat gggatcgcgg cgttcggcga acagcttttt gttttcggct
    44101 atcaggttgt tgccgtgggt atcaatagag acaatcagcg ggccgaactc tttgacccgg
    44161 cagacccaca gtgactccgg catcccgagc tctgtccagt gcacctcttc aatctcttcc
    44221 acctgggttg ccgccagcac cgcgcagcct gccgggaaaa tcacatgtag cgccttgaat
    44281 ttctgacagc cttcttcggt cagcggcccc attccgcctt tgccgaccac cagtttcacg
    44341 ccggtctgtt cgataaattc acgttcaaaa ctttccatac gcatactggt tgtcggaccg
    44401 acggaaacca tctcccattt gtcgccgttt ttgcgcacga tggggccagc gtggaaaatc
    44461 gctttgccgt tgagatcgta agggatcgga cgtttcagtt cgatcaaacg gcggtgacaa
    44521 acgtcacggc aggtcaccag cgtaccggtc aggtagatca catcgccgac gcgaatatct
    44581 tgcagatctt cagctttgat cggggttgtc aggatctttt tcataacgcg ctccgggtgt
    44641 gagacagatt ttcaaaggag agatcggcat gaaccagcag tgtgccgcga cgatgcgccc
    44701 agcagccggt agaaacagca acgccgatgg ttgacggatg gcgggcggca gattcgatat
    44761 gcacgcccat cactgaactg ttgccagtca gcccttgtgg accaatcccc agacggttga
    44821 gtccttcttc caggcgtagc tccagctctg ccgcttttgg attggggtgg cggctgccaa
    44881 tcgggcgcaa aatggctttt cgcgagagca cggcggcggt ttccaccgag gtggcgatgc
    44941 ccacgcccac cagtaccggt ggacaggcgt ttacggcgag ggtggagata ttttcgaaga
    45001 cgaatttcac tacgccttcg tagccttctg acggcattaa cactttcgag cggccaggta
    45061 gcgtgcagcc gccgcctgcc atgtaaactt cgatttccgc atcgtcaccg tcggggacga
    45121 tatcccaggt gacccacggt acgccgctac cggtattttt gccggtgttt acttcgtcaa
    45181 aaatttctac cgcattgtga cgcagtggcg ctttgatggt cgcctcttcc acggcttgtt
    45241 tgagtatgct ttgcagctcg ccaagcagtg ggaagcggga accgacttta acgaagaaca
    45301 taatctcccc ggtgtcctga caggcaggac gattcaggtc aatcgctttt tgcatgttgt
    45361 cgaacatcgt atggtagata attttcccca ttgacgacgt ttcggcatcc tttagctgtt
    45421 ttagtttatc caccacgtca tcaggcattc gggtagaaat catggcggta aagttagcga
    45481 caatctccgt caacttattc actgcctgtt gcttattact ttcgctcatc atggcaactc
    45541 cagtttctgt atttgtctgg aaaaattcac cgctacaaac taccactaaa aatgtggttt
    45601 tatatttttt gccaccgtga atatgtaatt aacagttcgt gaatcaacgc gaaaataaga
    45661 atgaatattg cgccagagca gggaatggca atgttttgcg aaatacactg cgcttatcac
    45721 aactgcggat aattaacgat gctgaatagc tggcctttag ccaaagactt gcaggtactg
    45781 gtagaaatcg tccacagcgg gagttttagt gctgcagcgg cgacgcttgg gcaaacgcct
    45841 gcatttgtca ccaagcggat ccaaatcctt gaaaatacgc tggcaacaac gctgctgaac
    45901 cgctccgctc gcggcgtggc gctgactgaa agtggccaac gttgttacga acatgcgctg
    45961 gaaatcctca cccaatatca gcgactggtt gatgacgtca cgcaaatcaa aacgcgcccg
    46021 gaggggatga ttcgtattgg ttgcagtttt ggttttgggc gcagccatat tgcgccagcc
    46081 attaccgaac ttatgcgcaa ttatcctgag ttacaggtgc attttgaatt gttcgatcgg
    46141 caaattgatt tagtgcagga taatattgat ctggatattc gtattaatga cgaaattcct
    46201 gattattata ttgcgcatct gttaacgaaa aataaaagaa tattatgcgc agcacctgag
    46261 tatctgcaaa aatatccgca gccacaatca ttacaggaat taagtcgtca tgactgtctg
    46321 gtgaccaaag agcgcgatat gacccacgga atatgggagt tggggaacgg tcaggagaaa
    46381 aaatcggtga aggtaagcgg gcatctttcc tccaatagcg gcgagattgt gctgcaatgg
    46441 gcgctggagg gcaaagggat tatgctgcgt tccgagtggg atgtgttgcc gtttctggag
    46501 agtggcaaac tggtgcgagt attgccggag tatgcacaaa gcgccaatat ctgggctgtt
    46561 taccgggagc cgctctatcg cagcatgaaa ttacgtgtct gcgtggaatt tctggcggca
    46621 tggtgccagc aacggctggg caagcccgat gaaggctatc aggtcatgta gatccaccag
    46681 aaatcactcg ggatcctttt cgcgctttct tttgaatttc gtccagattt ttgtctcctg
    46741 acgacgccac agacgctgga tgttgtcatg atgacgcagc aggatcaggc aagagagcat
    46801 cgaaaccggg aaggtgaatt gtggcttaaa ccaccagaca taaaacggag caatcagtgc
    46861 actgacaatc gctcccagcg acgagtatcc gctcaatagc acggtcagta accaggttcc
    46921 cgccattact ccggtgagat cccagccaat gggtgcgatg gcaccaaaag cggtagcaac
    46981 gccttttcct cctttaaatc cgaagaaaac gggccagatg tgtccaagac aggcggcgat
    47041 ggcaattaag cctagccaga aggggctgac acctaattca tacgcgcccc agacgggcaa
    47101 cattcctttc agaacgtcga aaatcagtac tgctacggct gctcccttgc caccgatacg
    47161 taacacattg gttgcgcctg gattgccgga gccgctggtt cgcggatcgg gcagcccaca
    47221 caagcggcaa accagaatgg cactggaaat ggagccgcag aggtacgcga tgaggatcat
    47281 tccaggcgcg attgcactca taagctgttc cgttttgaaa attcgtgttc aacgatgaat
    47341 ctgtggataa tacgcacatt tcgccggaag tggtatccgg ttagccaaaa agcaggcagg
    47401 acgtgatgga tattgtattt atagagcaac tttcggtaat caccactatt ggtgtttacg
    47461 actgggaaca gaccatcgaa cagaagttag tgttcgatat cgaaatggcg tgggataacc
    47521 gtaaagcggc gaaaagtgat gatgtggcgg attgcctcag ctacgctgac attgcagaaa
    47581 cggtggtcag ccacgtcgag ggggcacgtt ttgcgctggt ggagcgcgtg gcggaagagg
    47641 tggcggagct gctgttagca cgcttcaact cgccgtgggt gcgtatcaaa ctcagcaagc
    47701 caggcgcagt ggcgcgggcg gcgaatgttg gcgtaatcat tgagcgtggc aataatctga
    47761 aagaaaataa ttaattttac agctgttaaa ccaaacggtt ataacctggt catactgcag
    47821 tagttcggac aagcggtaca ttttaataat ttaggggttt attgatgagc gatatgcact
    47881 cgctgctgat agcggcaata ttgggtgtgg tcgaaggatt gacagaattt ctgccggtat
    47941 ccagcacggg ccatatgatt attgtcggtc acttgttggg gtttgagggc gacacggcga
    48001 aaacctttga agttgtgatc cagttaggat caattctggc ggtagtagtg atgttctggc
    48061 ggcgtctgtt tggcctgatt ggcatccact ttggccgccc gttgcagcac gaaggagaaa
    48121 gcaaaggtcg tttaacgctg atccacattt tgctggggat gattccggcg gtggtattgg
    48181 ggctgttgtt ccacgacacg attaagtcat tgtttaatcc gataaatgtg atgtatgcgc
    48241 tggtcgttgg cggtttgttg ctgattgccg ccgaatgcct gaagccgaaa gagccgcgtg
    48301 cgccgggtct tgatgatatg acctatcgtc aggcgtttat gattggctgt ttccagtgtc
    48361 tggcgctgtg gccgggtttc tcccgttccg gggcgaccat ttcaggtggg atgctgatgg
    48421 gggtgagccg ttacgctgct tccgagtttt cgttcctgct ggcggtgccg atgatgatgg
    48481 gcgcaacggc gctcgatctc tacaaaagct ggggcttcct gacaaccggc gatatcccga
    48541 tgtttgccgt tgggtttatc accgcttttg tggtggcgct gatagcgatt aaaaccttcc
    48601 tgcaattgat taagcgcatt tcgtttatcc cgttcgccat ttatcgcttt attgtggcgg
    48661 ctgcggtgta tgtcgtgttc ttttgattga ggaatagtgt cggatgcctg gcgttgtcca
    48721 cgattgcgta ggccgaataa ggcgttcacg ccgcatccgg caatcaacgc ctgatgcgac
    48781 gcttgcgcgt cttatcaggc ctacaactgt cgttgacatg tagccggata aggcgtttac
    48841 gccgcatccg gcaaaatgcc caatactcat tcaggctttg ggcaacgttg ttccttccag
    48901 ctggctaccg ccgcaatccg ccgtcgggtc agctcctcgc gaatctccac acctttaaat
    48961 cccgcttcaa cgacggcttt tgtcggcact gactgcgcca cttcccaggc ttcgcgcaac
    49021 cagcggcctt gcgggtagtc cgctgattca aaaccggttc tgccgcgcac gtcagcctcg
    49081 ctggtcagcg ccagttgctc gacacgctgc ggcttacgcc aggcatcgat ggaatcaaat
    49141 aatttgacga tggtttttgg gttcagcatt gggaaggtgt ggatgagatc gtgaaactca
    49201 gccaccagtc tggctaaatc gcgaatttca tttggcacgc gcagacgctg gcagagttgt
    49261 tccactaact taacacccgc cgggccatga ccatgatgac gcggccagag ttctggcggc
    49321 gtcagtcctt taccgagatc gtggcataaa gttgcgaaac ggacatcgac ctgcggactc
    49381 agcatcgccg ccattgagag cgtcattaag gtatgaatac ccgtatcgat ttccggatgc
    49441 cacttggcag gggccggaac accaaacagg gcgtcaattt ccgggaataa aacgcgaagt
    49501 gcgccgcaat cgcgcagtac ctggaagaac acctggggat tgcgggtggt gagggcgctt
    49561 tccgtctctt tccatacccg ttcaggcgtc aggtgttcca gttcacccgc atgggtcatc
    49621 tcgcgcatca gcgtcagagt ttcatcggca atacgaaaac cgaggtgggc ataacgcgca
    49681 gcaaaacgcg ccacgcgcag tacgcgtaac ggatcttcgc caaaagcggg agaaacatgg
    49741 cgcaacagac gattttgcag atcgcccaga ccgttgtacg ggtcgataat ctcgccgtta
    49801 tcgtcctggg ccagcgcatt aatggtcaga tcgcgacgct taagatcatc ttccagcgtg
    49861 acatccggtg cggcatagca ggtaaaaccg gtgtaaccgg agccggattt ccgttcggta
    49921 cgtgccagcg catactcttc atgcgtttgc ggatgcagaa acacaggaaa atcgcggcct
    49981 acctgctggt agcccgcgtc gagcatctcc tgtggcgtac tgccgaccac tacccaatct
    50041 ctgtctttga ccggtagccc taacaatgca tcccgaacag caccaccgac cagataaatc
    50101 ttcacgccat catcccttcg tcaaatacac aattcctaat aataagacag tgcaacgaag
    50161 aaggcgattt agttcatcca gcgatctttg cgtttgcggc ttgggatcag gtgcggcagt
    50221 accagaccga gcagcaagcc aagccccagc acgccaccgc catacataaa ccattgcatg
    50281 atgatggtgc gctgtttgtc atccagctgt acgctggcgg catcgacctt tttctgcgcg
    50341 acaatcagct cgtttttcag cttctgattt tcttctttta acccgttgat cacgctgtcg
    50401 ctctgcgcca ctttttgctg catttctgcg gtgcgctgat tccaggtgtt atcgatattg
    50461 gtgagtttat cggtcagggt tttgacctga ttttccagat ctggcacacg ggagcgcagg
    50521 cttggctcag tgctaagttg tttcaacggg atccaggcgg tacggccaga gctgtctttt
    50581 acctgggcat aattggtgtt ggcgtcagtt tgtaataagg tcacttcctc gccggcgtta
    50641 accgtgccca cgaggcgata atgatctccc ggaccgctgc ggacccaggt attcagttcg
    50701 tcggaaacat agcgcgtttc ttcagcgtgt gagacggcag tcgcgctaag tgcgagtaaa
    50761 gttaatccga tcaggcgtaa ttttggcatc aggctgtcgt tattgtcatg gaaagtggaa
    50821 cgatagtagt ggcatcagtg tcgctacgca aagcattcga catcaatcgg aatcctctgt
    50881 gtcaggtctt gccactgctt caaactttta cgcccgtcaa attgcccgtt gcatggcaat
    50941 ttggcgcaaa atactatcta ctgacaaaaa agatcgcatt aagttcgccg ttcatcgtca
    51001 cttatgtgac ataaccgata agtaaaggcc atggctcagg aaatcgaatt aaagtttatt
    51061 gttaatcaca gcgccgttga ggcgttgcgt gaccatctca atacgctggg cggcgagcac
    51121 catgaccccg tgcagttact gaatatttac tacgaaacgc cggataactg gctgcgtggg
    51181 cacgatatgg gcttacgtat tcgtggcgaa aacggtcgct atgagatgac catgaaagtt
    51241 gcaggaagag tgacaggcgg cttacaccag cgcccggaat ataacgtggc gttgagcgaa
    51301 ccgacgctcg acctggcgca gtttccctcg gaagtctggc cgaacggcga attgcctgcc
    51361 gatctcgctt cccgcgtaca accgctgttc agcaccgatt tttatcgcga aaaatggctg
    51421 gtggcggtcg atggcagccg aattgaaatc gctctcgatc agggggaggt gaaagcgggg
    51481 gaatttgctg aacctatctg tgagctggaa ctggaactgc ttagcggcga cacgcgcgcg
    51541 gtgctgaaac tggcgaacca actggtatcg caaaccggat tacgccaggg cagcctgagc
    51601 aaagcggcgc gtggctatca tctggcgcag ggcaatccgg cgcgtgaaat caaaccgacc
    51661 accattttgc atgttgcggc aaaagccgat gtggaacagg ggctggaagc ggcgctcgag
    51721 ctggcgttag cgcaatggca gtatcatgaa gaactgtggg tacgcggcaa cgatgcggcg
    51781 aaagaacagg tgctggcagc cattagcctg gtccgtcata cgctgatgct gttcggtggt
    51841 attgtgccgc gtaaagcgag cactcactta cgtgatttgc tgacccaatg cgaagcgacc
    51901 attgcttctg cggtttctgc cgtgacggcg gtctactcta ccgaaacggc aatggcgaag
    51961 ctggcgttga ccgaatggtt ggtaagcaaa gcatggcagc catttttaga tgccaaagcg
    52021 cagggcaaaa tcagcgactc cttcaaacgc tttgccgata tccatctttc ccgccatgcc
    52081 gctgaactga aaagcgtttt ctgccagccg ttaggcgatc gctaccgtga ccagttgcca
    52141 cgcctgacgc gtgatattga ctcaatactg ttgctggcgg gttactatga tcctgtcgtc
    52201 gcgcaagcct ggctggagaa ctggcagggg ctgcatcacg ctattgcgac cgggcaacgc
    52261 atcgaaattg aacatttccg taatgaggca aacaatcagg aaccgttctg gttgcacagc
    52321 ggaaaacgtt aatcaggcaa ggataaaatc gcttatgaag ccgctctctt caccgttaca
    52381 gcagtactgg cagaccgttg ttgagcggct gccagagcct ttagccgagg aatcacttag
    52441 tgcacaggcg aagtcagtac ttactttcag tgattttgtg caggacagca taagtgcgca
    52501 tccagagtgg ctgacggaac tggaaagcca accgccgcag gccgacgaat ggcagcatta
    52561 cgttgcatgg ttgcaggagg cgctcagtaa tgtgagtgac gaagccgggt taatgcgcga
    52621 gctgcggctg ttccgtcggc gcatcatggt acgtatcgcc tgggcgcaaa cgctggcact
    52681 ggttactgaa gagagcatat tgcagcagct cagctatctg gcggagacgc tgattgttgc
    52741 ggcgcgtgac tggctatatg acgcctgctg ccgcgagtgg ggaacgccgt gcaatgcgca
    52801 gggcgaagcg caaccgctgc tgattttagg catgggtaag ctgggcggcg gggaactgaa
    52861 tttctcctct gatatcgatc tgatttttgc ctggccggaa catggttgta cgcagggtgg
    52921 acgcagggaa ctggataacg cgcagttttt tacccgcatg gggcagcggc tgattaaagt
    52981 gctggatcaa ccaacgcagg atggcttcgt ctatcgcgtg gatatgcggc tgcgtccgtt
    53041 tggcgaaagt ggcccgctgg tgctgagctt tgccgcgttg gaagattatt accaggagca
    53101 ggggcgcgac tgggagcgtt acgcgatggt caaggcgcgg attatgggcg atagcgaagg
    53161 cgtctatgct aacgagttgc gtgcgatgct gcgcccgttt gttttccgtc gttacatcga
    53221 tttcagcgtg attcagtcgc tgcgcaacat gaaagggatg attgcccgtg aagtgcgtcg
    53281 acgtggtttg accgacaata tcaaactcgg cgcaggcggc attcgcgaaa ttgaatttat
    53341 cgttcaggtg ttccagctca ttcgcggcgg acgcgaaccg tcgctgcaat cgcgctcttt
    53401 actgccaacg ctcagcgcca ttgccgagct gcatctgctt tctgaaaacg atgctgaaca
    53461 attgcgagtg gcgtatctgt tcctgcggcg tctggaaaac ctgctgcaaa gcattaacga
    53521 cgaacaaacc cagacgcttc cttctgatga gcttaaccgt gcgcggctgg cgtgggcgat
    53581 ggactttgct gactggcagc aactgactgg ggcgctgacc ggacatatga ccaatgtgcg
    53641 ccgggtgttt aatgaattga ttggcgacga cgaaagtgaa actcaggaag agtcgctgtc
    53701 ggaacagtgg cgtgagctgt ggcaggacgc gttgcaggaa gatgacacta caccagtgct
    53761 ggcgcatctt agcgaggacg atcgcaaaca ggtgctgacg ctgattgctg atttccgcaa
    53821 agaactggat aagcgcacta tcgggccacg cgggcgtcag gtgctcgacc atctgatgcc
    53881 gcatctgcta agtgatgtct gtgcgcgtga agacgctgcc gttacgctgt cgcgcattac
    53941 cgccttgctg gtggggattg ttacccgcac cacctattta gaattgctca gtgaattccc
    54001 cgcggcgctt aaacatttga tttctctgtg tgccgcatcg ccgatgattg ccagccagct
    54061 tgcgcgttat ccattattgc tggatgaatt gctcgatcca aacacccttt accagccgac
    54121 ggcgaccgat gcctaccgcg atgagttgcg ccagtatttg ctgcgcgtgc cggaagatga
    54181 cgaagagcaa cagcttgagg cgctgcgtca gttcaaacag gcgcagctgt tacgcatcgc
    54241 cgcagcggat atcgccggta cgctaccggt gatgaaagtg agcgatcact taacctggct
    54301 ggcggaagcc atgatagatg ccgtcgttca gcaggcgtgg gttcaaatgg ttgcccgcta
    54361 cggtaagccg aatcacctga acgaacgcga agggcgtggt tttgcggtgg tcggctacgg
    54421 caagctgggc ggctgggagt taggctacag ttccgatctt gaccttatct tcctccatga
    54481 ttgcccaatg gatgcgatga ctgacggtga gcgggaaatc gacgggcggc agttttatct
    54541 gcgtctggcg caacgcatta tgcatctgtt cagtacgcgt acctcttccg gcattttgta
    54601 tgaagtggat gctcgactgc gtccgtccgg ggcggcggga atgctggtga catccgcaga
    54661 agcatttgcc gattatcaga aaaacgaggc ctggacgtgg gaacatcagg cgctggtgcg
    54721 tgcgcgtgta gtgtacggcg atccgcagct caccgcgcac tttgacgcgg tgcgtcgtga
    54781 gattatggcg ctgccgcgcg aaggcaaaac cctgcaaaca gaagtgcggg aaatgcgcga
    54841 gaaaatgcgc gctcatctcg gcaataaaca tcgcgatcgc tttgatatca aagctgatga
    54901 agggggaatt accgatatcg aatttattac ccaatatctg gtgttgcgct acgctcatga
    54961 aaaaccgaag ttaacgcgct ggtcagacaa cgtgcgtatt ctggaactgc tggcgcaaaa
    55021 cgacattatg gaagagcagg aagcgatggc gctgacccgt gcttacacta cgcttcgcga
    55081 tgaacttcat catctggcat tacaggaatt gccgggccat gtgtcggagg attgcttcac
    55141 cgcagagcgt gaactggtgc gggcaagctg gcagaagtgg ctggtggaag aatgaagtat
    55201 ggtattatcg cgcgcaaatt ttgaatctct caggagacag gaatgaaagt aacgctgcca
    55261 gagtttgaac gtgcaggagt gatggtggtt ggtgatgtga tgctggatcg ttactggtac
    55321 ggccccacca gtcgtatctc gccggaagcg ccggtgcccg tggttaaagt gaataccatc
    55381 gaagaacgtc cgggcggcgc ggctaacgtg gcgatgaata tcgcttctct cggtgctaat
    55441 gcacgcctgg tcgggttgac gggcattgac gatgcagcgc gcgcgctgag taaatctctg
    55501 gccgacgtca acgtcaaatg cgacttcgtt tctgtaccga cgcatccgac cattaccaaa
    55561 ttacgggtac tttcccgcaa ccaacagctg atccgtctgg attttgaaga aggtttcgaa
    55621 ggtgttgatc cgcagccgct gcacgagcgg attaatcagg cgctgagttc gattggcgcg
    55681 ctggtgcttt ctgactacgc caaaggtgcg ctggcaagcg tacagcagat gatccaactg
    55741 gcgcgtaaag cgggtgttcc ggtgctgatt gatccaaaag gtaccgattt tgagcgctac
    55801 cgcggcgcta cgctgttaac gccgaatctc tcggaatttg aagctgttgt cggtaaatgt
    55861 aagaccgaag aagagattgt tgagcgcggc atgaaactga ttgccgatta cgaactctcg
    55921 gctctgttag tgacccgttc cgaacagggt atgtcgctgc tgcaaccggg taaagcgccg
    55981 ctgcatatgc caacccaagc gcaggaagtg tatgacgtta ccggtgcggg cgacacggtg
    56041 attggcgtcc tggcggcaac gctggcagcg ggtaattcgc tggaagaagc ctgcttcttt
    56101 gccaatgcgg cggctggcgt ggtggtcggc aaactgggaa cctccacggt ttcgccgatc
    56161 gagctggaaa acgctgtacg tggacgcgca gataccggct ttggcgtgat gaccgaagag
    56221 gaactgaagc tggctgtagc ggcagcgcgt aaacgtggtg aaaaagtggt aatgaccaac
    56281 ggtgtcttcg atatcctaca tgccggacac gtctcttatc tggcaaatgc ccgcaagctg
    56341 ggtgaccgct tgattgttgc cgtcaacagc gatgcctcca ccaaacggct gaaaggggat
    56401 tcccgcccgg taaacccact cgaacagcgt atgattgtgc tgggcgcact ggaagcggtc
    56461 gactgggtag tgtcgtttga agaagacacg ccgcagcgtc tgatcgccgg gatcctgcca
    56521 gacctgctgg tgaaaggcgg tgactacaaa ccggaagaga tcgccgggag taaagaagtc
    56581 tgggccaacg gcggcgaagt gttggtgctc aactttgaag acggttgctc gaccactaac
    56641 attatcaaga agatccaaca ggataaaaaa ggctaaccgg aaagcggttc acagatctcg
    56701 cttcgtacat ggcaggccta ttgacgctgt tgttttttca acctagagta aaggaacaag
    56761 ggtaagggag gatttctccc ccctctgatg agttgttagt aagtcgggaa acttaacagt
    56821 aacaacacaa ccagtatgat gacgagcttc atcataaccc tttccttcta taaggcccct
    56881 tcctcgggag gggctttccc gtttcagccc cttgctgtct ccccgattgt tgatgagcgc
    56941 cggaccccgc gcaacgcttt ccctgaagca gttttgcctt ccccgagtgg tcacgcaaaa
    57001 tgctgcaacg tcctgagcat ttgcaccttt tagcggaacg cagatataaa aaatccccgg
    57061 ctttttgggc cagggatatg tgttaatgaa accttcttga cgctgatttt ttttcaacct
    57121 aaagtaaagg aacaagggtg agggaggatt tctcccccct ctgattggct gttaataagc
    57181 tgcgaaactt acgagtaaca acacaatcag tatgatgacg agcttcatca taaccctttc
    57241 cttatacaag gccccttctt cgggaggggc tttcccgttt cagccccttg ctgacttccc
    57301 gattgttgat gagtgccgga tcccgcgcaa tgctttccct gtggcagttt tgccttcccc
    57361 gagcggtcgc gcaaaacgtt gcaacaacct gatcatttgc agtttttagc ggaacgcaga
    57421 tataaaaaat ccccggcttt ttgggccagg gatgttcata gctgaaaatt tattctacgt
    57481 tttcggcgac gggagttgtt gaggttaagg gtgaagtcaa tgccgccagc gagccgcctg
    57541 aaacgccaat ttcattgagc aaggagtcaa tcagcggtgc ctgtgtgcgg taagagagtg
    57601 cggctgataa tgcttgttcc gccaggtttc cgccccccac attacccgtg tttgcatccc
    57661 ccgcagtacc accacgattc agaccatcga cctgaataat cttgataccg tcaatggact
    57721 tcattggttc cacggatttc tctattaccg caggtagcgc ctgcaacaag gccagtttga
    57781 atttaaggct ggtttgttcg tcagaaagta cgttgatagc atcgttcagc gcacgttgtg
    57841 cttctgcttc cgccaggcct tttttacgcg tagcttcggc taactcaacg atcgccgccg
    57901 cctgcatctc tgcggcttct ttttctgctt tcgcccgcac ggtcagttca accgctttgg
    57961 tttctgcatc ctgtgctgcg gcaatcagcg ccacctgctt ggcacgatcg gcttcggctg
    58021 tctggcgagt ggtttctacg ttttgttggg cgcttactgc ttctgcgagt gcaaggttag
    58081 cacgcgcttc tgcctgggac tgttgttccg atttggcggc aatagcgatc gatttcgtct
    58141 ggttagcgat ttcggtgacc tgctgctgtt cgatctcttt aatgcgaact tcacgttcgg
    58201 cctcaacctt tcttgagcgg acggcctgtt cgcggtcgat ttccgtttcc tgaatctgtc
    58261 gttcagccag aattcgcgtc tgctctgctt cacgacgacg ttcagcttca aaagccgcaa
    58321 ttttcgcatt ctgctcagcg gtacgggttt taacctgctg ctcctgctca agcgtcataa
    58381 acgcttcttg ttgttcaatc tccaacttgc gcgaaagtgc atcacggttt ttctcacgca
    58441 ccgcaacttc tacatcctgt tcaacttcgt tacgctcgcg gcgacggcgc tctgtctcct
    58501 gagtcagttt ggttaaacct tcggcgtcaa aggcattgtt aggattgaaa tgttctttcg
    58561 aggtctggtt aaagttggta agtgaaacgc tttccagttc caggccgttt ttcgacagat
    58621 cttcagctac ggtattttgt actccctgca cgaagttctc gcgggtatcc tgtaactcat
    58681 gcatggtcat ttgcgcagct gttgcacgga gggcatcgac aaatttatct tcaaccaaca
    58741 tacgtaagtc ttcaggcgac agggtgcgtt gccccagcgt ctgggcagcg gtggcaatcc
    58801 cttctactga aggttttacc cgcacaaaga aagcgactac tacatcgacg cgcatacgat
    58861 ctttcgtaat caggctatca atggttgagc ggctgacttc cagcttcaga gtattcatat
    58921 tgatggggat tatttcatga aagatcggca tcacgattgc gccaccgctc attaccactt
    58981 tttgcccacc taaaccagta cgaacaaaag cttgctctgc cgaagcgcga cgatagagcc
    59041 tggcgaaaat aattccaata ataaacagaa tgcatacggc aataatcgcg gtaaacatcc
    59101 aggagggtac agaattaaca atatcatcca ttattaaatt tccttttgat taataaatta
    59161 caatatttgg ggccagggat tgttttccgc caaatatcgc gttgcagaaa gtcggcaaat
    59221 aattaacact ttgtctcctt tagtgaaaat ttttccttct tcaggctcca gtagtaaata
    59281 atgtatttgg ccaaattgat cggtgagttt tccttcgcag ggattacctg aagtggcttg
    59341 atgaccggta attaatgcca tgctaccaat atattcttct tctgtaatag ctgaactatg
    59401 gtcgcgtgga atccaggggg caactatttt accggtataa tgcaccgcaa taatcgtaaa
    59461 cagtaaactg acaggaacca cgaacaggtt cgacagtggt gattgccaga ccatgatgca
    59521 ggcatgctgc aacaggatgc caataagacc aaaaaaacca gccagcaagc agagaacgac
    59581 gagagctggc aatcttccga tattgagata atgaagtgcc tggctaatat gacctgtggt
    59641 tatggaatca taatggtcaa gatgtgcatc aagagcaccg gagagcatgt gaccgcagat
    59701 aagcgcaaaa atttctaaaa ggccgatcaa cagtacaaag gaaatagcga aaagataagg
    59761 ggtattatag tcggcgaata aaatcatatg tcactccaat gtccatttag tgacgcaata
    59821 ttgactttgg gatagtttcc ttgcttacat ttatctttgt taataataac acatgttaga
    59881 gaagttctgg aagagaaagt cttttaatat ggttttttat ttttcactaa aaagtgtgat
    59941 cggggacaat atatttacgc acgttatgtt taaaggcact acactgattg gggaatactg
    60001 aaatcagaaa aagcatatgg ccttaaaagg tatgcgataa ccggaggatg tgcttatgga
    60061 tcatagtctt aattctttaa ataatttcga tttcctggcg cgtagttttg ccagaatgca
    60121 cgcagaaggt cgcccggtcg atattctggc cgttactggt aacatggatg aagaacatag
    60181 aacctggttt tgcgcacgtt atgcctggta ttgccaacag atgatgcaaa ccagagaact
    60241 tgaattagaa cactgatatt acgggccgtc tggcccgttt ttgtgttgct ttattctgct
    60301 ttatcaatag gcgggatagc cggaggagat tgaagatctg ctaccgaatt attgcgcgct
    60361 tccagttcac tgatgcgctg ctccagtaac gccagttttt cacgagtccg caacaggact
    60421 tgcgtctgaa cgtcgaattc ttcgcggctt accagatcga gacgcgtcag ctgcgcttgt
    60481 agggtttggc ggattttttt ctccacatct tccccgaact ccctgattcc tttaggcatt
    60541 gattcgtgaa cctggcgagc gatttgctca atttttttcg ggtcaatcat tgtagtttcc
    60601 ctgtactgat aggtgttgag tgccattgta gtgcgataag ggtaagtcat aaaccagaat
    60661 tatgtgaagc tatgcgttgc tgccgctaat cattagcgtt atagtgaatc cgcttattct
    60721 cagggcgggg cgaaattccc caccggcggt aaatcaactc agttgaaagc ccgcgagcgc
    60781 tttgggtgcg aactcaaagg acagcagatc cggtgtaatt ccggggccga cggttagagt
    60841 ccggatggga gagagtaacg attctgtcgg gcatggaccc gctcacgtta ttttggctat
    60901 atgccgccac tcctaagact gccctgattc tggtaaccat aattttagtg aggttttttt
    60961 accatgaatc agacgctact ttcctctttt ggtacgcctt tcgaacgtgt tgaaaatgca
    61021 ctggctgcgc tgcgtgaagg acgcggtgta atggtgcttg atgatgaaga ccgtgaaaac
    61081 gaaggtgata tgatcttccc ggcagaaacc atgactgttg agcagatggc gctgaccatt
    61141 cgccacggta gcggtattgt ttgcctgtgc attactgaag atcgccgtaa acaactcgat
    61201 ctgccaatga tggtagaaaa taacaccagc acctatggca ccggttttac cgtgaccatt
    61261 gaagcggctg aaggtgtgac taccggtgtt tctgccgctg accgtattac gaccgttcgc
    61321 gcagcgattg ccgatggcgc aaaaccttct gatctgaatc gcccgggcca cgttttcccg
    61381 ctccgcgcgc aggctggtgg tgtactgacg cgtggcggtc atactgaagc aactattgat
    61441 ctaatgacgc tggcaggctt caaaccggct ggtgtgctgt gtgagctgac taatgacgat
    61501 ggcacgatgg cgcgtgcacc agagtgcatt gagtttgcca ataaacacaa tatggcgctt
    61561 gtgactattg aagacctggt ggcataccgt caggcacatg agcgtaaagc cagctgaaaa
    61621 ccgctgctta atttactgcc ttaatcaaga aaccgaagtt gtagcaggct tcggttttta
    61681 tttttccctg ctatgcaaga tgttaattcc gcttctcttt ctatgagaaa atttcattaa
    61741 tatcaggcat tctttttcat tatgatcaaa atcatataat tgttccgtta ttatgcgacc
    61801 atatgaatat atccgaaaaa ggatatgtga aatttatgcg ctaaatgttt agtattgtta
    61861 aatattaagg gtaaaaggat gtttatcgcc tggtactgga ttgtattgat tgctctggtt
    61921 gtggtgggtt atttcctgca tttgaaacgt tattgtcggg cgtttcgcca ggacagagac
    61981 gcactgcttg aagcgcggaa caaatactta aacagtacga gagaggagac agccgaaaag
    62041 gtagaataag gctttcgtga agctggcaga agttgtccat gcgctggaca acttctgatg
    62101 ttgcttttaa ccaattcccg ccgtttgtag cagcactaaa ctgaatccca tcactgacat
    62161 tccacatagt acgccatagc tggggttatt attagggtca atctctttgg cgagcggcat
    62221 taattcatca accgagagcg ccaccataat tcctgcaacc gccgccatga ttgccgccat
    62281 gaccacgggg gaaatcatgc taccgaggat taaccacgcc agcacaccac caagaatttc
    62341 tgccaatcca gaaatccccg cccacagaat tgccgtgcgt ttagaacccg ttgctgcata
    62401 aaccgggcct gcaaccgcca gaccttcagg aatattgtgc aacgcgacgg ccagtgcgat
    62461 gccaaatccc agctccaggt tgctgcttgc cgtgacaaag gtggcaatcc cttccgggaa
    62521 gttatgcaga ctgataccga gagtgagcag aatggctgtg cgcttgatcg attttggcaa
    62581 cggctgcacc gatttttgca ttaaatcctg cggatgcgca tgtggcaaca tgcggtccag
    62641 gccaaaatag ccaagcagac cgaagataaa cattccataa cccaacacag gcgacattcc
    62701 ttcagcagct agtgcggcag gaagcatttc cattaatgag atgagcaaca tgatccccgc
    62761 cgcaaatcct agcgaaaacg ccagtaagcg gttcgagggt ttttgcccga gaacgccgag
    62821 aaacgcgcca ataaacgtgg ctgcccccgc cagtatggtc agaatgagag gtactgacat
    62881 cgacaactcc ttataaatct tcaccttcag gcagatcgtc aaaataactg atgatcatca
    62941 tcattgttat ccgggttctt actccgccga acaagcgtat tgtgaggata tcaaaatgac
    63001 acctttagtt aaggatatca tcatgtcttc aacacgtatg ccagcattgt ttttaggtca
    63061 cggtagtccg atgaacgtgc tggaagataa tttgtatacc cgcagctggc agaagttggg
    63121 gatgacattg ccacgcccgc aagcgattgt ggtggtttcg gctcactggt ttacccgtgg
    63181 aacaggagtg accgcgatgg agacgccgcc cacgattcat gactttggtg gcttcccgca
    63241 ggcgctgtac gatacgcatt atcctgctcc gggttcgcct gcgctggcac agcatctggt
    63301 tgagctgtta gcgccggtcc cggtgacgct ggataaagaa gtctggggct ttgaccacgg
    63361 ttcctggggc gtgctgatta agatgtatcc tgacgcggat atcccgatgg tgcagttgag
    63421 tatcgatagt agcaaacctg ccgcctggca tttcgaaatg gggcgaaaac tcgcggcgct
    63481 gcgtgatgaa ggaataatgt tggtcgccag cggcaacgtg gtgcataacc tgcgtacagt
    63541 gaagtggcac ggtgatagtt caccgtatcc gtgggcgacg tcgtttaatg agtatgtgaa
    63601 agcgaatctg acgtggcaag ggccagtgga acaacatcct ctggtgaatt acctcgacca
    63661 tgaaggtggc gcgttatcga acccaacgcc agagcactat ctgccgttgt tgtatgtgtt
    63721 aggtgcgtgg gatgggcagg agccaattac cattccggtc gatggtatag aaatgggcag
    63781 tttaagtatg ctgtcggtgc agataggtta acaattaccg gatggcgaat gccatccggc
    63841 atcattgatt attcaacaaa aatatgtgga taaaaccgag acatatcctg ggtgatcaat
    63901 gcacggtctt cacgaatgcc aattccggcg ggttgatcgt tcaccagcca gctaccaatc
    63961 agcatatagc tgtcgccgaa tttcggtaac gggtggaatt gctgaacaat catcccttct
    64021 tcgccatacg gaccttccgc tgcttcaatg gttttgccgt tctcaatgat cgacacgttt
    64081 gcgccttcac gggagaagat cggtttaacc acatattttt ccatttgcgg atgatcatct
    64141 tccgcaaaat aagcgggcag caggttcggg tgattcggga acatctccca cagtagcggt
    64201 agaagtgcct tgttggagat aatgctcttc cacgccggtt ccagccagcg tacgcctgca
    64261 tcctccagct tggtggagaa catctcacgc aacataaatt cccacggata cagcttgaac
    64321 aggttggaaa ttacctgatc ctgtaaatcc gtgaactgac ctttttcacc taacccgata
    64381 tcatcgatgt agaggaactc agtagcaatt tcagcttccg ttgcgcagtc ctgcaaatac
    64441 tgaatggttc cgcgatcttc cactgtgtcg cgacagcagg tgagatgcag caactggaag
    64501 ccatactgtt cacgcagctc aacgaagcga tcgatcagtt tttcttgcag actgttaaac
    64561 tggtcgctgc cctccggcaa gttaccggcg ttaagctgat cttccagcca gatccactga
    64621 aagaacgccg cctcgtatag tgacgttggc gtatcggcgt tattttccag aagtttaggt
    64681 tcgccagtgc catcccacgc cagatcaaga cgcgaataaa gcgatggctg gtgagtcagc
    64741 catgattgac gcacaaaact ccaggtgtgt tttggaatgc ggaatttggt catcagctca
    64801 tcgctggcga tcactttttc caccactttc aggcacatct ggtgcagttc ggcggtgact
    64861 tcttccagct tttcaacctg ggcgagggtc aacttgtagt aagcatcttc acaccagtac
    64921 ggctcgccgt acatggtgtg aaaattgaaa ccgtattcgt gggctttctc acgccagtcc
    64981 gggcgctcgg taatactgac tctttccatc ggtatcagcc acccattgaa cgagaagagg
    65041 tgccggttgc gctacgctgc atagtgcttt gtttggcaac agattcacca aaaccgccac
    65101 gggtaacggt agtggtggtc gccggttttg gtgccattgc cgtcttcggt acggtcatgg
    65161 tgcggcctgg ctgggctgcg ccatagtttt tacccgtcgc gtcggtatat ttaccgtaag
    65221 ccggactggc tgggtttttc gaggagaaca gcggctgctg tgcaaatccc gcgccgccgc
    65281 ccatcagacg ccccatcatg taaccggcca tcagcggcat ccagaaactc ccgctggatt
    65341 gctgagcctg cgcctggttt tctggtgcca tgccagcctg ggctggtgcc tgctggcact
    65401 gaccttcacc aaattcagca acacagtctt cacgggtggc gtatttcggc gcagtacgtt
    65461 cggcttcttt cagcgcattg ttgtacgcgg tggtacattc ggcgcttttg cctgggtttg
    65521 cagctgaaca gtcgtcagca ttttgataga gagacactgt ttcatcactc ttttcacagc
    65581 cagccagcat aaaaacagtg gcaactgcga gagcgactgg tgtcagatgg cgtgcgctcc
    65641 agtttttgcg gaacgatgcg tggcgtatgg attttgtccg tttcattttt gtcttccggg
    65701 accagtggta aatacccatc agaatagagg atggctggtc gaaattgaag cgagaagggg
    65761 gaagaatgcg gcagataacc cgtatcttta cgttgcctta cgttcagacg gggccgaagc
    65821 cccgtcgtcg tcatcagtta cggaaagggt tatgaccgtt actggtggta gtgcgtgcgg
    65881 atgtttgctg aacgacgggg gcagggctat caggcgcata accatcagca atagcattct
    65941 gttccggcgt ttgcggggca acgttttccg gattagtgga aaccggtttg ctcagcgcat
    66001 tgttcagtgc cagcagatcc tgctcgttca acgtacccag ggctgactta atattcagct
    66061 gattaatcag gtagttataa cgcgcattcg ccagctcttg cttggcgttg tacagcgtgg
    66121 tggtcgcatc caacacatca acaatggtac gcgtaccgac cgagtagccc gcttccatcg
    66181 cgtctaatga gctttgagcg gaaactacgg cttgtttgta ggcgttaata ctactgatag
    66241 aagcattaat gttgttgaag gaggaacgta cggtttgcac gacgctacga tgtgcgcttt
    66301 ccagttgctc gctggcacca acaaagttgt actgtgcctg tttcacctgc gagttaacca
    66361 ttccgccctg ataaatcggc agcgagaagc tcaggccaac tttgttctgg cccatattgc
    66421 tatcgtcata ctgggtacca gcggcaccac gggttttcga accgctataa gaggtgtcag
    66481 aaatcccggt agaagccgtt aaatccagcg tcggtaagtg accatcctgc gcctggcgaa
    66541 tttgctcgcg cgccaggtcc tggctcaagc gtgcctgtaa cagcgacagg ttgcgttttt
    66601 cggcttcttt cagcagcgcg ttaaccggct gtggtttgtc ggttttaaag ttttcgacat
    66661 tcagcgccgc cagttccgga tagtagttac cggtgatctg gcgcagctgc tctaccgcgt
    66721 tatcaaggtt attacgtgcg gtcacttcgt tcgccagcac ggtatcgtac tgcgcgcggg
    66781 cgttctgcac gtcggtgatc gctaccaggc ccacgttaaa acgttgggtg gtttgatcta
    66841 attgacggta gatcgcttct ttttgcgcct gtgtatagga aagaacgtca atagcattca
    66901 acacgttgaa ataagcggtc gcggtgttga ggatcaaggt ttgctgatcg gtctgatacg
    66961 tgacgtcctg aatccctgct gctttttcct gcagcgttaa cgcacgccat ttcgacatat
    67021 caaaaatgga ttgagttaac tgcagggacg cactggtcgc gttagagttg atgccgttcg
    67081 cgtcgcggta gccgttgcta taggtgtaat ctgcacctaa acctagctgt ggcagtaatg
    67141 gactgcgcgc ttcattaatt ttttcaaagg cagcatcacg atcggcggca gacttacgca
    67201 attccgggtt actaaggcgt gcttgctgat aaacttgcat caggttctcg gcctggctca
    67261 acgaactgaa cccagaaagg ctcaggccga taagaatggg gagcaatttc ttcatttgca
    67321 ttccttgtgg tgaagcagta tttagcgcga tcaaactgta aaattattgc ggattctagc
    67381 agaagccgct accgcaaaag gttggcgtta cgtgccatag ggcgttaatg tgcctcaatt
    67441 taacacatga ctgctcaaac ggcagtcaaa cgtcgctgaa attcacattt aattcactat
    67501 tagtgccagg acattaacaa tgcttaagcc agacaacctg cccgttacat ttggcaaaaa
    67561 cgatgtagaa attattgcac gagaaacact ttatcgcggc tttttttcat tagatcttta
    67621 tagatttcgt catcgtctat tcaacgggca aatgagtcat gaggtgcggc gggaaatttt
    67681 tgagcgcggt cacgccgcag tcttgctacc ctttgaccca gtgcgtgatg aagttgtgct
    67741 gattgagcag attcggattg ccgcatacga caccagtgaa accccctggc tactggagat
    67801 ggttgccggg atgattgaag agggtgaaag tgtggaagat gttgcccgtc gcgaagcgat
    67861 tgaagaggcg ggactgatag tcaaacggac caaaccggtg ttaagtttcc tggcaagccc
    67921 ggggggcacc agtgagcgtt cgtcaattat ggtgggcgaa gtggacgcca cgaccgcaag
    67981 cggtattcat ggtctggctg atgaaaacga agatattcgc gttcatgtgg taagccggga
    68041 acaggcatac cagtgggtag aagaggggaa aatcgacaac gcagcgtcgg tcatcgcttt
    68101 gcaatggctg cagctgcatc atcaagcgtt aaaaaatgag tgggcataaa tgaagcgtta
    68161 cacacctgac tttcctgaaa tgatgcgcct gtgcgagatg aacttttcac aattgcgccg
    68221 tttgttaccg cgcaatgacg cacccggcga aactgtaagc tatcaggtgg caaacgcaca
    68281 atatcggctg acgattgtgg aatcgacccg atacactacc ctggtgacaa tagaacagac
    68341 tgcgcccgcg atcagttact ggagccttcc gtcaatgacg gtgcgtctgt atcatgacgc
    68401 gatggtggct gaagtgtgtt caagccagca gatttttcgc ttcaaagcgc ggtatgatta
    68461 tcctaataaa aagttgcatc aacgcgacga aaagcatcaa attaaccagt ttttagcgga
    68521 ctggttgcga tactgtttag cacatggagc gatggcgatt ccggtttact agcgtcgtga
    68581 aacctaagga caccatttgg aaagcctgtt aacccttcct ctggctggtg aggccagagt
    68641 caggatttta caaattaccg atactcacct gtttgcacaa aagcacgaag ccctgttagg
    68701 ggtaaacacc tgggaaagct accaggcggt gctggaggcg attcgtccac accagcacga
    68761 attcgacctg attgtcgcga caggtgattt agcgcaggat caatcctctg cggcctatca
    68821 gcatttcgct gaaggcatcg caagttttcg tgcgccctgc gtctggctgc cgggcaacca
    68881 cgatttccag cccgcgatgt acagcgcgtt acaggatgcg ggtatatccc cggcgaagcg
    68941 cgtgtttatt ggtgagcaat ggcaaatcct gttgctggat agccaggtgt ttggcgtgcc
    69001 gcacggtgag ctgagcgagt ttcagcttga gtggctggaa cgtaaactgg ccgatgcgcc
    69061 agaacgccat acgttgctgc tgctgcatca tcatccgcta cctgcgggtt gtagttggct
    69121 cgatcaacac agtctgcgta acgcgggcga actggatacc gtgctggcga agtttccgca
    69181 cgtcaaatac ttgctgtgcg gtcatattca tcaggagctg gatctcgact ggaacggtcg
    69241 ccgcctgctg gcaacgccgt cgacctgtgt gcagtttaag ccgcactgtt ccaactttac
    69301 gttggacacc atcgcgcccg gctggcgtac tctcgagtta catgctgatg gcacgctgac
    69361 caccgaggtg catcgcctgg cggacacacg tttccaacct gataccgctt cagaaggcta
    69421 ctgatgtcta cgcttcttta tttacacggt ttcaacagct cgccgcgctc tgcaaaagcg
    69481 agcttgttaa aaaactggct ggcggaacat caccctgacg ttgagatgat cattccgcag
    69541 ttgccgccgt atccttccga cgcggcagag ctgctggaat ccattgtcct ggaacatggc
    69601 ggcgattcgc tgggtattgt cggttcgtca ctggggggat attacgccac ctggttgtca
    69661 caatgtttta tgctgcccgc agtggtggta aacccggcgg tgcgcccgtt tgaactgctg
    69721 acggactatc tcggtcagaa cgagaacccc tacaccgggc agcaatatgt gctagagtca
    69781 cgccatattt acgatctcaa agtcatgcag attgacccgc tggaagcgcc ggatttgatc
    69841 tggctgctgc aacagacggg agatgaagtg ctggattacc gccaggcggt ggcgtactac
    69901 gcttcctgcc gccagactgt catagaaggc ggcaaccacg cattcacggg cttcgaagat
    69961 tatttcaacc cgatcgtcga ttttcttggt ctgcaccatc tctgacgatc aactcgaatt
    70021 gctaacttaa accatgacgc aaacttataa cgctgatgcc attgaggtac tcaccgggct
    70081 tgagccggtt cgccgccgtc cggggatgta taccgatacc actcgcccta accatttggg
    70141 gcaagaagtc attgataaca gtgtggatga agcactggcg ggtcacgcaa aacgcgtgga
    70201 cgttatttta catgctgacc agtcgttaga agttattgac gatgggcgcg ggatgccggt
    70261 ggatattcac ccggaagagg gtgtaccggc ggttgaactg attctttgcc gtctgcatgc
    70321 aggcggtaaa ttctctaaca aaaattacca gttctctggc ggcctgcatg gcgtggggat
    70381 ttcggtggtt aacgccctgt cgaagcgcgt agaagttaac gtgcgccgcg atggtcaggt
    70441 ttataacatc gcctttgaaa atggcgaaaa ggtgcaggat ttacaggttg tcggcacttg
    70501 cggtaaacgc aataccggca ccagcgtcca tttctggccg gatgaaacct tcttcgatag
    70561 cccgcgtttt tctgtttcac gcctgacgca tgtgctgaaa gccaaagcag tactgtgccc
    70621 cggcgttgag attaccttta aagatgagat caacaacacc gaacagcgct ggtgctatca
    70681 ggacggtctg aatgattacc tggcggaagc ggtaaacggt ttaccgacgc tgccagaaaa
    70741 accgtttatc ggtaatttcg ctggcgatac tgaagcggtg gactgggcgc tactgtggct
    70801 gccggaaggc ggtgaactgc tgaccgaaag ctacgtcaac cttatcccaa cgatgcaggg
    70861 cggtacccat gttaacggtc tgcgtcaggg cctgttggac gcgatgcgtg agttctgtga
    70921 ataccgcaac attcttccgc gcggtgtaaa gttgtcggcg gaagatatct gggatcgctg
    70981 cgcctatgtg ctgtcagtaa aaatgcagga tccgcagttt gccgggcaga ccaaagagcg
    71041 tctctcttcg cgtcagtgcg cggcattcgt ttctggcgtg gtgaaagatg cctttatcct
    71101 gtggctgaac cagaacgttc aggcggcgga gttgctggca gagatggcga tttccagcgc
    71161 ccagcgtcgt atgcgtgcgg ctaaaaaagt ggtgcgtaaa aagctgacca gcggcccggc
    71221 gctgcctggc aaactggctg attgtaccgc gcaggacctt aaccgtaccg aactgttcct
    71281 cgtggaaggt gactccgcag gcggatctgc caagcaggcg cgcgatcgtg aatatcaggc
    71341 gatcatgccg ctgaaaggta agatccttaa cacctgggaa gtttcttccg acgaagtgct
    71401 ggcctcgcag gaagtgcatg atatttcggt agcgatcggt atcgatcctg acagcgatga
    71461 tctgagccag cttcgttatg gcaaaatctg tatcctcgcg gatgcggact ctgatggtct
    71521 gcacattgcc acgctgctct gcgctttgtt cgtaaaacac ttccgcgcgt tggtgaaaca
    71581 cggtcacgtt tacgtcgcac tgccaccgct ctaccgtatt gatctcggga aagaggttta
    71641 ttacgcgctg acggaagaag agaaagaggg cgtacttgag caattaaaac gcaagaaagg
    71701 caagccgaac gtccagcgtt ttaaaggtct gggggaaatg aacccgatgc aattgcgtga
    71761 aaccacgctt gatccgaaca ctcgccgtct ggtgcagttg actatcgatg atgaagacga
    71821 tcagcgtact gacgcgatga tggatatgct gctggcgaag aaacgctcgg aagatcgccg
    71881 caactggttg caagagaaag gcgacatggc ggagattgag gtctgataat cctcccgcca
    71941 gaagaataaa acatagcccg gtatacaagt gtataccggg cttttttgat caaaattcca
    72001 ttttcaccgt gaattgcact tcacgcggat cgccaatctg attccccaga ttatttgtgg
    72061 cgatggaaga ggtgtaatac gttttatcaa acaggttttt gacgtttaat tgcagtgtga
    72121 ccggatactg caatttcatt ttgtatgcgg cgaaggcatc ggcaacgaaa tagcctggca
    72181 gataatagtc agccccattg gttgccgaac gacggcttac gccatgtcca ccaccgccaa
    72241 acgtcagtgt gttattgcct ggcatgttat gaatgtcata ggtcaggaat agcgaaccgg
    72301 tatgacgagg aacattcggc aatggtttcc ctgcataatc aggatcttcc agaaccttag
    72361 catcggtata gccgtagctg gcaatgatat taatgttttc agttaatgct cccgcaaggt
    72421 cgacttctac ccctcttgaa cgaacgcggc ctgccgtttt ggcgatggtt tcatcaccaa
    72481 tactttcggt atacaacacg ttacgtttat ggatatcaaa cagcgcaata tctgcggtta
    72541 taccatcgaa cagctcgaat tttgccccga cttcgtaagc attagatgat tccggtggaa
    72601 gatctccgat gtagctggca attgacgatt gcggcataaa tgtttgcgaa taattagcaa
    72661 ataaggatac cgatggcgtc agtttgtaga ctaaccccag tttgggcgtc cattgttcat
    72721 cgcggctgtc agtattgaca ttaaaaggac ggcctttacc cgcatactgc gtgtaatact
    72781 gatagcggat cccggcgacg gcaatccagt tatcggtcag atagagcgcg tcctgtgcat
    72841 aagctgagta gctctcctgt ttgatcgtct gatcactgtc cgacgccgaa accgttgtgc
    72901 atttgctggt attgccataa acggggttgt atatattgaa atctttagcg tttttacagc
    72961 gaatcatatc tgtgcgcaga agatcataat attcatatga caccccaccc agaatctcat
    73021 tatagaaccc agcaatatca acattccctt gcagatccgc acgagtagaa tgcatacgct
    73081 gggtagatcc ctgagttgca tcaacacgcc gtatcagcgt tcctgtcgtt gcatcatacg
    73141 cggtaacgcg agcctgattg tcgctgtatt tatcctggct gtagctgtaa tcaaagcgcg
    73201 ctgtccactg gctattgaga tgatattctg cgttgagttg cgccagatcg gactgaccat
    73261 ctgtaatatt aaacggttcg tcaaaacgta tttttcgatc aacgtttaca ggctgtttcg
    73321 tcgtaaggtc gaaaatcgtt ccacgatcga acggagtttt atagtcccga tgggaataga
    73381 gcatggttac tgttgcatta tcaccaaacc aggtgagtga cggggcaata aatgtactgc
    73441 gctctttacc gaagtttcgc cagtaatctt catcctgcac ttcccccgtc aggcggtatg
    73501 ccagctgagt gccttcaatg ggacctgtga tatcaagttg cccagtgcct ccgccaaaac
    73561 tggaggacgt ggctgagaca gaaccatgga atgttttttc cgggcgcttg gtcacgacgt
    73621 taatcaatcc accaggatcg agaatgccat acagcgtgga ggtcggacct tttagcactt
    73681 ccacacgttc tgtggcggcg ttgaaactgc gaggaagtac agttcgcagg ccgttggtca
    73741 tgatggagcc atcccggtta gcaccaaaac cacgacgtac aaaggcgtcc tgagttccgc
    73801 ctaatgtatt ggtctgtacc acgttactga cgttataaag cgcttcatcc agcgtcgttg
    73861 cattctgatt ttccagaacc tgatcgctaa ccgtattgac cacctgcggg atatccagca
    73921 tcggcatatc ggttaatgtc gccgtggaag tgctcagagg ttgataacca tcggtcgcct
    73981 cagttgcggt atttgcatcc gctgtaacag tgattgtttc gccatctttg gtcgttgcag
    74041 tgtcggttgc atgaatcatt ggtgctgcaa agagcagtga aaagagcgcc cgacctttga
    74101 ttcctgagaa tgaaggtgtg aacttagcca tttagtctgt tatcccaaaa actcacggat
    74161 tgtcgtgagt gaaaattgtt tcttgttgct cactaagcac tatccgtttg ttatttcacc
    74221 atcggatatg taattgagaa tcattcaacc atgcggtaat gtagtagtaa atgagaaata
    74281 acaaaaaatt gctacatgtg tttttttgag a
//
LOCUS       DAPGRN010000008        73844 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPGRN010000008 DAPGRN010000000
VERSION     DAPGRN010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.008"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RHW33_000592"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RHW33_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV1644138.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RHW33_000593"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RHW33_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV1644139.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RHW33_000594"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RHW33_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV1644140.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RHW33_000595"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RHW33_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV1644141.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RHW33_000596"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RHW33_000596"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV1644142.1"
                     /translation="MLHTLHRSPWLTDFPALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLIGQISNDVIPIDYTDFVRLTVKHTSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RHW33_000597"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RHW33_000597"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV1644143.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDELAVFFIADGV
                     FQLLPGQKPGAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELGNYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RHW33_000598"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RHW33_000598"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV1644144.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQQGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="RHW33_000599"
     CDS             complement(4598..5320)
                     /locus_tag="RHW33_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1644145.1"
                     /translation="MSRSLLTNETSELDLLDQRPFEQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RHW33_000600"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RHW33_000600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1644146.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="RHW33_000601"
     CDS             6520..6738
                     /locus_tag="RHW33_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV1644147.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7371)
                     /gene="slyD"
                     /locus_tag="RHW33_000602"
     CDS             complement(6787..7371)
                     /gene="slyD"
                     /locus_tag="RHW33_000602"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV1644148.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVSADNAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGEHDHHH
                     DHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7466..7666)
                     /locus_tag="RHW33_000603"
     CDS             complement(7466..7666)
                     /locus_tag="RHW33_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV1644149.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEVRDHVRKDEQVIGIFHPD"
     gene            complement(7675..9480)
                     /gene="kefB"
                     /locus_tag="RHW33_000604"
     CDS             complement(7675..9480)
                     /gene="kefB"
                     /locus_tag="RHW33_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV1644150.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDNPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9480..10031)
                     /gene="kefG"
                     /locus_tag="RHW33_000605"
     CDS             complement(9480..10031)
                     /gene="kefG"
                     /locus_tag="RHW33_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV1644151.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10162..12075
                     /locus_tag="RHW33_000606"
     CDS             10162..12075
                     /locus_tag="RHW33_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1644152.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQTMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12075..13097
                     /locus_tag="RHW33_000607"
     CDS             12075..13097
                     /locus_tag="RHW33_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1644153.1"
                     /translation="MTQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13091..13309
                     /locus_tag="RHW33_000608"
     CDS             13091..13309
                     /locus_tag="RHW33_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV1644154.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13363..14232
                     /locus_tag="RHW33_000609"
     CDS             13363..14232
                     /locus_tag="RHW33_000609"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV1644155.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14287..14691)
                     /locus_tag="RHW33_000610"
     CDS             complement(14287..14691)
                     /locus_tag="RHW33_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV1644156.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14993..15625
                     /gene="crp"
                     /locus_tag="RHW33_000611"
     CDS             14993..15625
                     /gene="crp"
                     /locus_tag="RHW33_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV1644157.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15676..17766
                     /locus_tag="RHW33_000612"
     CDS             15676..17766
                     /locus_tag="RHW33_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1644158.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17833..19053)
                     /gene="argD/dapC"
                     /locus_tag="RHW33_000613"
     CDS             complement(17833..19053)
                     /gene="argD/dapC"
                     /locus_tag="RHW33_000613"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV1644159.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIETTFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKSVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTAEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19139..19702)
                     /gene="pabA"
                     /locus_tag="RHW33_000614"
     CDS             complement(19139..19702)
                     /gene="pabA"
                     /locus_tag="RHW33_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV1644160.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19734..20336)
                     /locus_tag="RHW33_000615"
     CDS             complement(19734..20336)
                     /locus_tag="RHW33_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV1644161.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20326..20493)
                     /locus_tag="RHW33_000616"
     CDS             complement(20326..20493)
                     /locus_tag="RHW33_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV1644162.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20598..21170)
                     /gene="ppiA"
                     /locus_tag="RHW33_000617"
                     /gene_synonym="rotA"
     CDS             complement(20598..21170)
                     /gene="ppiA"
                     /locus_tag="RHW33_000617"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV1644163.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21441..22622
                     /gene="tsgA"
                     /locus_tag="RHW33_000618"
     CDS             21441..22622
                     /gene="tsgA"
                     /locus_tag="RHW33_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV1644164.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22884..25427
                     /gene="nirB"
                     /locus_tag="RHW33_000619"
     CDS             22884..25427
                     /gene="nirB"
                     /locus_tag="RHW33_000619"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV1644165.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25424..25750
                     /gene="nirD"
                     /locus_tag="RHW33_000620"
     CDS             25424..25750
                     /gene="nirD"
                     /locus_tag="RHW33_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV1644166.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25876..26682
                     /gene="nirC"
                     /locus_tag="RHW33_000621"
     CDS             25876..26682
                     /gene="nirC"
                     /locus_tag="RHW33_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV1644167.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26701..28074
                     /gene="cobA"
                     /locus_tag="RHW33_000622"
     CDS             26701..28074
                     /gene="cobA"
                     /locus_tag="RHW33_000622"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV1644168.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28329..28496
                     /locus_tag="RHW33_000623"
     CDS             28329..28496
                     /locus_tag="RHW33_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV1644169.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28741..30129
                     /gene="frlA"
                     /locus_tag="RHW33_000624"
     CDS             28741..30129
                     /gene="frlA"
                     /locus_tag="RHW33_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HDV1644170.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            30150..31172
                     /gene="frlB"
                     /locus_tag="RHW33_000625"
     CDS             30150..31172
                     /gene="frlB"
                     /locus_tag="RHW33_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HDV1644171.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPHAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGEQASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31223..32050
                     /gene="frlC"
                     /locus_tag="RHW33_000626"
     CDS             31223..32050
                     /gene="frlC"
                     /locus_tag="RHW33_000626"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HDV1644172.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYASQALERFRALLPEDE"
     gene            32050..32835
                     /gene="frlD"
                     /locus_tag="RHW33_000627"
     CDS             32050..32835
                     /gene="frlD"
                     /locus_tag="RHW33_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDV1644173.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIKPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPELVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32935..33666
                     /gene="phoB"
                     /locus_tag="RHW33_000628"
     CDS             32935..33666
                     /gene="phoB"
                     /locus_tag="RHW33_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HDV1644174.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33797..34801)
                     /gene="trpS"
                     /locus_tag="RHW33_000629"
     CDS             complement(33797..34801)
                     /gene="trpS"
                     /locus_tag="RHW33_000629"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV1644175.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(34794..35552)
                     /gene="gph"
                     /locus_tag="RHW33_000630"
     CDS             complement(34794..35552)
                     /gene="gph"
                     /locus_tag="RHW33_000630"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV1644176.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(35545..36222)
                     /gene="rpe"
                     /locus_tag="RHW33_000631"
     CDS             complement(35545..36222)
                     /gene="rpe"
                     /locus_tag="RHW33_000631"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV1644177.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(36240..37076)
                     /gene="dam"
                     /locus_tag="RHW33_000632"
     CDS             complement(36240..37076)
                     /gene="dam"
                     /locus_tag="RHW33_000632"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV1644178.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(37183..38469)
                     /gene="damX"
                     /locus_tag="RHW33_000633"
     CDS             complement(37183..38469)
                     /gene="damX"
                     /locus_tag="RHW33_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV1644179.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(38561..39649)
                     /gene="aroB"
                     /locus_tag="RHW33_000634"
     CDS             complement(38561..39649)
                     /gene="aroB"
                     /locus_tag="RHW33_000634"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV1644180.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(39706..40227)
                     /gene="aroK"
                     /locus_tag="RHW33_000635"
     CDS             complement(39706..40227)
                     /gene="aroK"
                     /locus_tag="RHW33_000635"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV1644181.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(40628..41866)
                     /gene="hofQ"
                     /locus_tag="RHW33_000636"
     CDS             complement(40628..41866)
                     /gene="hofQ"
                     /locus_tag="RHW33_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV1644182.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRNITLQYADAGELAKAGEKLLSAKGNMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(41778..42182)
                     /gene="hofP"
                     /locus_tag="RHW33_000637"
     CDS             complement(41778..42182)
                     /gene="hofP"
                     /locus_tag="RHW33_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV1644183.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(42172..42612)
                     /gene="hofO"
                     /locus_tag="RHW33_000638"
     CDS             complement(42172..42612)
                     /gene="hofO"
                     /locus_tag="RHW33_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV1644184.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQHWATLYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(42596..43135)
                     /gene="hofN"
                     /locus_tag="RHW33_000639"
     CDS             complement(42596..43135)
                     /gene="hofN"
                     /locus_tag="RHW33_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV1644185.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRLQRQRQRQFTRDWQSELEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(43135..43914)
                     /gene="hofM"
                     /locus_tag="RHW33_000640"
     CDS             complement(43135..43914)
                     /gene="hofM"
                     /locus_tag="RHW33_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV1644186.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            44034..46586
                     /gene="mrcA"
                     /locus_tag="RHW33_000641"
     CDS             44034..46586
                     /gene="mrcA"
                     /locus_tag="RHW33_000641"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV1644187.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(46751..47311)
                     /gene="nudE"
                     /locus_tag="RHW33_000642"
     CDS             complement(46751..47311)
                     /gene="nudE"
                     /locus_tag="RHW33_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV1644188.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            47631..49766
                     /gene="igaA"
                     /locus_tag="RHW33_000643"
     CDS             47631..49766
                     /gene="igaA"
                     /locus_tag="RHW33_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV1644189.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNHQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            49831..50499
                     /gene="yrfG"
                     /locus_tag="RHW33_000644"
     CDS             49831..50499
                     /gene="yrfG"
                     /locus_tag="RHW33_000644"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV1644190.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            50510..50911
                     /gene="hslR"
                     /locus_tag="RHW33_000645"
     CDS             50510..50911
                     /gene="hslR"
                     /locus_tag="RHW33_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV1644191.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            50936..51814
                     /gene="hslO"
                     /locus_tag="RHW33_000646"
     CDS             50936..51814
                     /gene="hslO"
                     /locus_tag="RHW33_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV1644192.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(51877..53601)
                     /locus_tag="RHW33_000647"
     CDS             complement(51877..53601)
                     /locus_tag="RHW33_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV1644193.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWDELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQQQISEKV
                     LAENVLIAPGSGKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            53980..55602
                     /gene="pckA"
                     /locus_tag="RHW33_000648"
     CDS             53980..55602
                     /gene="pckA"
                     /locus_tag="RHW33_000648"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV1644194.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVDEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(55678..57030)
                     /gene="envZ"
                     /locus_tag="RHW33_000649"
     CDS             complement(55678..57030)
                     /gene="envZ"
                     /locus_tag="RHW33_000649"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV1644195.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(57027..57746)
                     /gene="ompR"
                     /locus_tag="RHW33_000650"
     CDS             complement(57027..57746)
                     /gene="ompR"
                     /locus_tag="RHW33_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV1644196.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            57974..58450
                     /gene="greB"
                     /locus_tag="RHW33_000651"
     CDS             57974..58450
                     /gene="greB"
                     /locus_tag="RHW33_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV1644197.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            58547..60868
                     /locus_tag="RHW33_000652"
     CDS             58547..60868
                     /locus_tag="RHW33_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV1644198.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEGEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGRKAQPAGNSAMMDALAAAMGKKR"
     gene            61306..61533
                     /gene="feoA"
                     /locus_tag="RHW33_000653"
     CDS             61306..61533
                     /gene="feoA"
                     /locus_tag="RHW33_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV1644199.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            61550..63871
                     /gene="feoB"
                     /locus_tag="RHW33_000654"
     CDS             61550..63871
                     /gene="feoB"
                     /locus_tag="RHW33_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV1644200.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYTQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            63871..64107
                     /gene="feoC"
                     /locus_tag="RHW33_000655"
     CDS             63871..64107
                     /gene="feoC"
                     /locus_tag="RHW33_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV1644201.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            64310..65188
                     /gene="rpnA"
                     /locus_tag="RHW33_000656"
     CDS             64310..65188
                     /gene="rpnA"
                     /locus_tag="RHW33_000656"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HDV1644202.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            complement(65215..65985)
                     /gene="bioH"
                     /locus_tag="RHW33_000657"
     CDS             complement(65215..65985)
                     /gene="bioH"
                     /locus_tag="RHW33_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV1644203.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGAMSLADMAEAVLRQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            66023..66706
                     /gene="gntX"
                     /locus_tag="RHW33_000658"
     CDS             66023..66706
                     /gene="gntX"
                     /locus_tag="RHW33_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV1644204.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRRRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            66765..67340
                     /gene="nfuA"
                     /locus_tag="RHW33_000659"
     CDS             66765..67340
                     /gene="nfuA"
                     /locus_tag="RHW33_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV1644205.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            67701..69017
                     /gene="gntT"
                     /locus_tag="RHW33_000660"
     CDS             67701..69017
                     /gene="gntT"
                     /locus_tag="RHW33_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV1644206.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(69062..71146)
                     /gene="malQ"
                     /locus_tag="RHW33_000661"
     CDS             complement(69062..71146)
                     /gene="malQ"
                     /locus_tag="RHW33_000661"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV1644207.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQEDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(71156..73549)
                     /gene="malP"
                     /locus_tag="RHW33_000662"
     CDS             complement(71156..73549)
                     /gene="malP"
                     /locus_tag="RHW33_000662"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV1644208.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AIASKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQENK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        17603 a        20017 c        18754 g        17470 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 aatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatacgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgagc acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gaacgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcggcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttaggg gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcggtg tcgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccgtca ccaggccatc tggctggtgt gctttaccgt aagtctgacg
     3601 aaatcagtat agtcaattgg gatgacatcg ttcgaaattt gaccaatcaa accgcgggca
     3661 atgaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttgccgt caactgcggc agttacgcca tcctgcaata acaatagttc gtcgccctca
     3781 ctgagcagac gcagcagtgc aggaaaatcc gtcagccagg gtgagcgatg taatgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaattac atcgtagttg ccaagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccacgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcaccgc accgggcttt tgtcctggca gtagctgaaa
     4081 aacgccatca gcaataaaga agacggccag ctcgtcagtt aacgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgcc cggcagcggt accatgaggc acagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tccctgctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccgttt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaagg tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctgctcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tatcactttt
     6781 tcggtattag tggcaaccgc aaccgccgtt acctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccgtgacc accgcagcaa ccgtcgtgat cgtggtgatg
     6901 atcgtgctca ccgtgaacgt gaccatgagc cagttcttct tcggttgctt cgcgaatcgc
     6961 cacaacttca acgttgaatt tcaggttctg accagccagc atgtggttac cgtcaaccac
     7021 aacgtgatcg tcttcaaccg cagtgatttc aaccggtacc ggaccctggt cggtttcagc
     7081 caggaaacgc atacctacct gcagttcatc aacgcccata aatacgtctt taggaacgcg
     7141 ctgcaccagg ttttcgtcgt actgaccgta agcgttatcc gcactaacag ctacgtcaaa
     7201 tttgtcgcca acttcatgac cttccagcgc tgtttccagg ccagagatca gagaaccgtg
     7261 accatgcagg tagtccagcg gcgcacttac cggagactca tcaaccaata caccgtcttc
     7321 tgtacgtacc tgataggcca ggctgaccac caggtctttt gctactttca tgatatctcc
     7381 tgagcatggg aagaatagtg gcgcagattg tagcggaatt ctgcagccgt gtactcacta
     7441 gcttaaaaaa tctcggcgca tatcgctagt ccggatgaaa aatcccgatc acttgctcat
     7501 ctttgcgaac gtgatcgcgg acttctttat cagcttctcg catctggtgt ccgcacttaa
     7561 cacattcaac aatatcaata ttattttcgc gccacatcgc cattgaatcc tgcgcctgac
     7621 aggccgggca ttttgcgccc gcaataaaac gttttcggat tgccatcttt acctctactc
     7681 aaattcatcc cagccgtcca gctggcgtcg ttcttgttgc atttcacgct ggaaaatctc
     7741 ttccagttcg cgtcgggctt ccctggcgcg agaaatttgt acggtatcgg catgcattgg
     7801 gatgagctct cgcagcatac gcatatccag gcggcgaaaa tgcagttgcg cccgctgtgc
     7861 ctgatgtgga tgcatgccaa gcgtgaccag cgtcttgcgc cccagctcta acgcactgga
     7921 gaatgtttca cgggaaaact gcgtcacccc tgcctgtaat aactcatgcg cttccacacg
     7981 tccgcgcgct cgcgcaagaa tatgcaaatg cggaaagtgc tgctggcata tttccaccag
     8041 cttcatggtg tcttccggct cgttacaggt aatgacgata gactcagcgg cctctgcacc
     8101 cgcagaacgt aaaagatcta cctgcgtggc gtcgccgtaa taaactttgt agccgtattt
     8161 acgcatcagg ttaacggcgc tgatatcccg ctccagcacg gtaatgcgca ttttatttgc
     8221 catcagcaaa cgaccaatca cctgaccaaa acgcccaaag cccacgacaa tgacctgggg
     8281 gttatcatcg ttgacccacg gtttttcgtc ttcttcttcc ggtccgttaa actggcggga
     8341 tagccattta tccaccagct tcatcagcag cggcgtggtc atcatggaaa gcgtcaccgt
     8401 caccagcaac agcgccatct ggtcgccctg gaataagcgt tgtgaagaag cggtagaaaa
     8461 gaggacaaag gcaaactcac cgccctgact caacacgcca gcaaactgca tccgctctga
     8521 gctacgcacg ccatacaatc gcgccagcag atacaacacg agaattttca ccgccaccag
     8581 cacaaccacg cttatcacta cccacaacag atgggtataa agcaccccga gattgagcga
     8641 catgccgaca gagataaaga acaaaccgag cagcaagcct ttaaagggat cgatagccgt
     8701 ttccagttca tggcgatatt cactttccgc cagcagcacg cctgcaataa acgtaccgag
     8761 cgccatcgac agccccaacg catccataaa caatgcggaa cccaatacca gcagcagcgt
     8821 cgcggcggtg aacacttccc gcacgccaga agctgcaata aaacggaata ccggacgcaa
     8881 taaatagcgc ccgccaatca gcatgccgac aaacgccagc accttcatgc cgatcttcat
     8941 ccagtcgaaa tgttcgtctg ccgaacccgc caacaacggc actaacgcca gtgccgggat
     9001 caccgccaga tcctgaaaca gcagtaccga aaacccgagc tgcccggatt cgctgcgatt
     9061 catccctttc tcacgcatca attgcaacgc cattgcggtc gaagacatcg caaggccaat
     9121 gccaccgacc accgccgcct gccaggcgaa atccgtcagc atcaataatc ccgccagcaa
     9181 cgccgcgctt aacagcacct gtgccgcgcc tacgccaaaa atcgagcgcc gcagttgcca
     9241 aagcttggag ggattcaact caaggccgat gataaacatc aggaacacca cgccgagttc
     9301 cgaaaagtgg aggatctcat cgacgtcgct aataaatccc agcccccacg ggccaattgc
     9361 aatccctgcc agcaaatatc ccaacacagc gccaataccc agccgcgatg ccaacggcac
     9421 cgcagccacc gccgcgaaga gaaacagcac tcctgcaagt aaaaaatcgg aaccttccat
     9481 cagcggcctc ctggagacag cggatttgcc agccagtcac cgtaggctct ggcgtggctc
     9541 gccagctcct gtgcgctttg ccgtctcgcc caataaataa tgatgggact taaccaatgc
     9601 atccggcaca tgcccgccgc cagttcaaag gggcgcagga catcgctcat cgggtagcga
     9661 ttcaacgcgt cataacggta agcactttcc ggctcgccgg tggtaatcac actacgccag
     9721 tactttcccg ccagttggtt tcctcccggc ccgctggcaa aaccacggct taatacccgg
     9781 tccagccact ctttcagtag cgccgggcag ctataggtat aaagaggatg ctgaaagaca
     9841 atcacctcgt gctcgcgcag taatgcctgc tcacggggga tatcaataaa aaaatcggga
     9901 tagtgcgcgt aaaggtcgtg cacggtaaca ttgctgagct gcgtggccgg tttaagcagt
     9961 acccggtttg ccaccgagtc ctgagattcc ggatgggcat acagcagcaa aactttcgct
    10021 ggctgagaca tcatccccct cccggtatgg tttttgtgta tagtcgctgt tttgggctac
    10081 cattgcgccc ggtgcggcag ctcgcccata cattacatta tcataatgat aagttaacat
    10141 agtctgaaca tacggcacct tatgattgtt ttctcctcgt tacaaattcg tcgcggcgtg
    10201 cgcgtcctgc tggataatgc caccgccacc atcaaccccg ggcagaaagt cggcctggtg
    10261 ggtaaaaacg gctgtggtaa atctaccctg ctggcattgc tgaaaaatga aatcagcgcc
    10321 gacggcggca gctacacctt tccgggaagc tggcaactgg cgtgggtgaa tcaggaaacg
    10381 ccggcgttac cgcaagcggc gctggaatat gtcattgacg gagaccgtga atatcgtcaa
    10441 ctggaagcgc agctacacga cgccaacgaa cgtaacgacg ggcacgccat tgcgaccatt
    10501 catggcaagc tggatgctat tgacgcatgg agtattcgct cccgtgccgc cagcctgctg
    10561 cacggcctcg gtttcagcaa tgaacaactg gagcgcccgg taagtgattt ctctggtggc
    10621 tggcgtatgc gtcttaacct tgcccaggcg ctgatttgcc gttcagactt gctgctgctc
    10681 gacgaaccga ctaaccacct cgatctcgat gccgttatct ggctggaaaa atggctgaag
    10741 agctatcagg gcacgctgat cctgatctcc cacgaccgcg acttcctcga tccgatcgtc
    10801 gataaaatta ttcatatcga acagcaaacc atgttcgagt acaccggcaa ctacagttcg
    10861 tttgaagtac agcgcgccac ccgtctggcg cagcaacaag cgatgtacga aagccagcag
    10921 gaacgcgtag cgcatctgca aagttatatc gaccgtttcc gtgccaaagc caccaaagcg
    10981 aagcaggccc agagccgcat taagatgctc gagcgtatgg agctgattgc cccggcgcac
    11041 gtcgacaatc cgttccgctt tagcttccgc gcgccggaaa gtctgccaaa tccgttactg
    11101 aagatggaaa aggtcagcgc aggctatggc gatcgcatta ttctcgactc gattaaactg
    11161 aatctggtcc ccggttcgcg cattggtctg ttaggccgca acggcgcggg taaatcgaca
    11221 ttaatcaaac tgttagccgg tgaacttgcg ccagtcagtg gtgaaatagg cctggcaaaa
    11281 gggatcaagc tcggctactt cgcccagcat caacttgaat acctgcgcgc cgacgaatcg
    11341 cctattcaac atctggcacg tttagcgccg caggagctgg agcaaaaact gcgtgactac
    11401 ctcggcggct ttggtttcca gggcgataaa gtaaccgaag aaacgcgccg cttctcaggt
    11461 ggggaaaaag cccgcctggt gctggcatta attgtctggc agcggccgaa tctgctgctg
    11521 ctcgacgaac cgactaacca ccttgacctc gacatgcgtc aggcactcac cgaagcatta
    11581 atcgagtttg aaggcgcgct ggttgtcgtt tcgcacgacc gtcatttgct gcgttccacc
    11641 actgacgacc tctacctggt tcacgatcgt aaagtcgaac cgttcgacgg cgatctggaa
    11701 gattatcaac agtggttgag cgacgtacaa aagcaggaaa accagaccga cgaggcgcca
    11761 aaagagaacg cgaacagcgc ccaggcacgt aaagatcaga agcgccggga agcggagctg
    11821 cgtgcgcaaa cccagccact gcgtaaagag attgcccgtc tggaaaaaga gatggagaag
    11881 ctgaacgcgc aactggcgca ggcggaagag aaactcggcg acagcgaact gtatgaccag
    11941 agccgtaaag cggagttgac cgcctgcctg caacagcaag ccagcgccaa atccggcctg
    12001 gaagagtgcg aaatggcgtg gctggaagcc caggagcagc ttgagcagat gctgctggaa
    12061 ggccaaagca actgatgacg cagataacga cgaccgatgc caatgaattc agcagcagtg
    12121 ctgaattcac ccctatgcgc ggctttagca attgtcatct gcaaaccatg ctgccgcgtc
    12181 tgtttcgtcg caaggtgaaa ttcaccccgt actggcagcg gctggagttg cccgacggcg
    12241 attttgtcga tctcgcgtgg agtgaagccc cagcacaggc gcggcataaa ccgcgtttag
    12301 tggtatttca cgggctggaa ggcagtctca atagccctta cgcccacggt ctggttgagg
    12361 cggcgcaaaa gcgcggctgg ctgggcgtgg tgatgcattt tcgcggatgc agcggtgaac
    12421 caaaccgtat gcaccgcatt taccattcgg gcgaaaccga agacgccagt tggtttttac
    12481 gctggctgca acgcgagttt ggtcatgcgc caacggctgc cgtcggctat tcgctcggcg
    12541 gtaatatgct ggcctgtttg ctggcaaaag aaggcaatga tctcccggtt gatgcggcgg
    12601 tgattgtctc tgcgccgttt atgctggagg cctgtagtta ccatatggaa aagggctttt
    12661 cccgcgttta tcagcgttac ttgctgaacc tgttaaaagc caatgccgca cgcaagctgg
    12721 ctgcctaccc cggaacgctg ccgataaatc tcgcgcaatt aaaatcggta cgtcgcatcc
    12781 gtgaatttga cgatctcatc accgccagaa ttcacggcta cgctgacgct atcgactatt
    12841 atcgtcagtg tagcgccatg ccgatgctga accggatcgc caaaccgacg ctgattattc
    12901 acgctaaaga cgatccgttt atggatcatc aggtgatccc gaaaccggaa agtctccccc
    12961 cgcaggtgga gtatcaactg actgaacatg gcggtcatgt tggctttatt ggcggtacat
    13021 tacttcatcc gcaaatgtgg ctggagtcac gcattcctga ctggttaaca acgtatctgg
    13081 aggcgaaatc atgttgattc cgtggcaaga cctctccccc gaaacgctgg aaaatttaat
    13141 tgaaagcttt gtgttacgtg aaggcaccga ttatggtgaa catgagcgta cacttgaaca
    13201 gaaagtcgcc gacgtcaaac gccagctaca gtgcggagaa gcggtactgg tatggtcgga
    13261 gctgcacgaa acggtcaata tcatgccgcg cagtcagttt cgcgaataac ctgcaaccgt
    13321 cggcggacga gataataatg atagctaagt catggagtta ctatgtctgc caaacatccg
    13381 gtcattgcgg taacaggatc cagcggcgcg gggaccacca ccaccagcct cgcgtttcgt
    13441 aaaatattcg cgcagttaaa tctgcatgca gccgaggtgg aaggcgacag ttttcaccgt
    13501 tacacccgcc cggaaatgga catggcgatc cgcaaagcgc gcgacgccgg gcggcatatc
    13561 agctacttcg gccccgaagc gaacgacttc ggcctgctgg aacaaacctt cattgaatac
    13621 ggtcagagcg gcaaagggaa atctcgcaaa tatctgcata cctacgacga agccgtaccc
    13681 tggaatcagg taccgggaac attcaccccc tggcaacctt taccagaacc cactgatgta
    13741 ctgttttatg aaggtttaca cggcggcgta gtcacgccac agcataacgt tgcgcagcat
    13801 gtggacttac tggtcggcgt ggtgcctatc gttaaccttg agtggattca aaaactgatc
    13861 cgcgacacca gcgagcgcgg gcactcacga gaagcagtga tggactcagt agtgcgttca
    13921 atggaagact atatcaacta catcacaccg cagttttccc gcacccatct taacttccag
    13981 cgcgttccca ccgtcgacac ttcaaacccg ttcgcggcaa aaggtatccc gtcgctggat
    14041 gaaagctttg tggtgatcca ttttcgtaat ctggaaggga tcgatttccc ctggctgctg
    14101 gcgatgttgc aaggctcatt catttcccac atcaatacgt tagtggtgcc cggcggcaaa
    14161 atgggtctgg caatggaatt aattatgctg ccgctggtgc aacgattgat ggaaggaaag
    14221 aaaatcgagt aactctgcta ttacgccgga taaaatacta tccggcttca caacgggata
    14281 gttaaatcac gcggcaacca cttcatacga gtgagtaata ttcaccgctt tttccagcat
    14341 caacgccact gaacaatatt tctcagcaga gagatcaacc gcacgcgcaa ccgctgcgtc
    14401 tttcaggtcg cgaccggtga cgataaaatg cagattaatg tgcgtaaaca ggcgtggtgc
    14461 ctcttcgcgg cgttcagagg tcaattttac ttcacaatcg accacatcct gacgcccttt
    14521 ttgcaggatc gaaaccacat cgatggcact gcaacccccc gccgccatca acaccatttc
    14581 catcggactt ggtgctttat cgcctgagtt gccgtccatt aaaatctggt gaccagaggc
    14641 ggattcgccc agaaaagtta acccttcgac ccacttcact cgcgcttgca tttttgctac
    14701 tccactgcgt caattttcct gacagagtac gcgtactaac caaatcgcgc aacggaaggc
    14761 gacctgggtc atgctgaagc gagacaccag gagacacaaa gcgaaagcta tgctaaaaca
    14821 gtcaggatgc tacagtaata cattgatgta ctgcatgtat gcaaaggacg tcacattacc
    14881 gtgcagtaca gttgatagcc ccttcccagg tagcgggaag catatttcgg caatccagag
    14941 acagcggcgt tatctggctc tggagaaagc ttataacaga ggataaccgc gcatggtgct
    15001 tggcaaaccg caaacagacc cgactctcga atggttcttg tctcattgcc acattcataa
    15061 gtacccatcc aagagcacgc ttattcacca gggtgaaaaa gcggaaacgc tgtactacat
    15121 cgttaaaggc tctgtggcag tgctgatcaa agacgaagag ggtaaagaaa tgatcctctc
    15181 ctatctgaat cagggtgatt ttattggcga actgggcctg tttgaagagg gccaggaacg
    15241 tagcgcatgg gtacgtgcga aaaccgcctg tgaagtggct gaaatttcgt acaaaaaatt
    15301 tcgccaattg attcaggtaa acccggacat tctgatgcgt ctgtctgcac agatggcgcg
    15361 tcgtctgcaa gtcacttcag agaaagtggg caacctggcg ttcctcgacg tgacgggccg
    15421 cattgcacag actctgctga atctggcaaa acaaccagac gctatgactc acccggacgg
    15481 tatgcaaatc aaaattaccc gtcaggaaat cggtcagatt gtcggctgtt ctcgtgaaac
    15541 cgtgggacgc attctgaaga tgcttgaaga tcagaacctg atctccgcac acggtaaaac
    15601 catcgtcgtt tacggcactc gttaatcccg tcggagtggc gcgttacctg gtagcgcgcc
    15661 attttgtttc ccccgatgtg gcgcagactg atttatcacc ccgatatcaa ctatgcacta
    15721 cgacaaacgc tggtgctatg tttgcccgtg gccgttgggt taatgcttgg cgaattacga
    15781 ttcggtctgc tcttctctct cgttcctgcc tgttgcaata ttgcgggcct tgatacgcct
    15841 cataaacgtt ttttcaaacg cttaatcatt ggtgcgtcgc tgtttgccac ctgtagctta
    15901 ctgacacagc tgctactggc aaaagatgtt cccctgccct ttttgctgac cggattaacg
    15961 ctggtacttg gcgtcactgc tgagctgggg ccattgcacg caaaattgct tcctgcatcg
    16021 ctgctcgccg ccatttttac cctcagtctg gcgggataca tgccggtctg ggaaccgttg
    16081 ctcatctatg cgttgggcac tctctggtac ggattgttta actggttttg gttctggatc
    16141 tggcgcgaac aaccgctgcg cgagtcacta agtctgctgt accgtgaact ggcagattat
    16201 tgtgaagcca aatacagcct gcttacccag cacaccgacc ctgaaaaagc gctgccgccg
    16261 ctgctggtgc gccagcaaaa agcggtcgat ctaattaccc agtgctatca gcaaatgcat
    16321 atgctttccg cgcaaaataa tactgactac aagcggatgc tgcgtatttt ccaggaggcg
    16381 ctggatttac aggaacatat ttcggtcagt ttgcatcagc cggaagaggt gcaaaagctg
    16441 gtcgagcgta gccatgcgga agaagttatc cgctggaatg cgcaaaccgt cgccgctcgc
    16501 ctgcgcgtgc tggctgatga cattctttac catcgcctgc caacacgttt tacgatggaa
    16561 aagcaaattg gcgcactgga aaaaatcgcc cgccagcatc cggataatcc ggttgggcaa
    16621 ttctgctact ggcatttcag ccgcatcgcc cgcgtgctgc gcacccaaaa accgctctat
    16681 gcccgtgact tactggccga taaacagcgg cgaatgccgt tacttccggc gctgaaaagt
    16741 tatttgtcac taaagtctcc ggcgctacgc aatgccggac gactcagtgt gatgttaagc
    16801 gttgccagct tgatgggcac cgcgctacat ctgccgaagt cgtactggat cctgatgacg
    16861 gtattgctgg tgacgcaaaa tggctatggc gcaacccgtc tgaggattgt gaatcgctcc
    16921 gtgggaaccg tggtcgggtt aatcattgcg ggcgtggcgc tgcactttaa aattcccgaa
    16981 ggttacaccc tgaccttgat gctgattacc acccttgcca gctacctgat attgcgcaaa
    17041 aactacggct gggcgacggt cggttttact attaccgcag tgtataccct gcaactgttg
    17101 tggctgaacg gtgagcagta catccttccg cgtcttatcg ataccattat tggttgttta
    17161 attgctttcg gtggtaccgt ctggctgtgg ccacagtggc agagcgggtt attacgtaaa
    17221 aacgcccatg atgctttaga agcctatcag gaagcgattc gcttgattct gagcgaggac
    17281 ccgcaaccaa cgccactggc ctggcagcga atgcgggtaa atcaggcgca taacactctg
    17341 tataactcat tgaatcaggc gatgcaggaa ccggcgttta acagccatta tctggcagat
    17401 atgaaactgt gggtaacgca cagtcagttt attgttgagc atattaatgc catgaccacg
    17461 ctggcgcggg aacaccgggc attgccacct gaactggcac aagagtattt acagtcttgt
    17521 gaaatcgcca ttcagcgttg tcagcagcga ctggagtatg acgaaccggg gagttctggc
    17581 gatgccaata tcatggatgc gccggagatg cagccgcacg aaggcgcggc aggtacgctg
    17641 gagcagcatt tacagcgggt tattggtcat ctgaacacca tgcacaccat ttcgtcgatg
    17701 gcatggcgtc agcgaccgca tcacgggatt tggctgagtc gcaagttgcg ggattcgaag
    17761 gcgtaatgca gactaaacta tcggataagg tgttcacgcc gcatccgaca tcttttgcct
    17821 gatgcgtctt atttacgccc caacaacttt cgccaccgcg tgggcgaaac gttgcatccc
    17881 ttcatcgata tccgcatctt ccaccaccag cgacggtgca aaacgcatca catccggtcc
    17941 ggcattcagc accattaccc cagcctctgc gcccgcatac aggaaatcac gcgcccgacc
    18001 tttgtactgt ggtttcagct ccgcgccaat taacagcccc ataccgcgaa tatcgctaaa
    18061 cacatcgtac tgctgatcga tcttctgcaa atggtcaaca aaacgctggc gtttcgcctg
    18121 aatgccttcc agcacctccg ccgtattgat gatatcaaac gccgcgcccg ctactgcaca
    18181 ggccagtgga ttaccgccgt aggtagaacc gtgagaacca ggatgaaacg cagaagcaat
    18241 ttccgccgtg gtcagcatgg cgctaatcgg gaagccgccg cctaacgctt tcgcagaggt
    18301 cagaatatcc ggcgtcacgc cgtagtgcat gtaagcaaac aaatcgccag tccgccccat
    18361 cccgcactgt acttcatcaa acaccaataa tgcctgatgt tgatcgcaca actcacgcaa
    18421 gccctgcaaa aactctggcg tcgctgccgt cacaccaccc tcgccctgga tcggctcaac
    18481 caccaccgca caggtgtgat catccatcac cgatttcact gcgtggagat cgttaaaggg
    18541 aacgtggatg atgtctgctg gttttggccc aaagccgtcg gaatattttg gctgcccacc
    18601 caccgaaacg gtaaacagcg agcgaccatg aaaagcgtta tggaaggcaa taattttggt
    18661 tttgaacggg ctatgacgca cacaggcgta atggcgtgcc agtttaaagg cggtttcgtt
    18721 agcttccgtg ccggagttca taaacaccac gcgttcggca aacgtcgtct caatcagttt
    18781 gcgcccaaga cgcagcgccg gttcattggt gaaaacgtta ctgatatgcc acagagtttc
    18841 gccctgggtt tttaacgcgt tcaccagcgc aggatggcaa tggcccaacg ccgtaactgc
    18901 aatgccaccc gcgaaatcga catactcctt gccttgctga tcccagattc ggctgccctg
    18961 accttttacc ggaatgaact ctgccggagc ataaatcggc aggatcactt catcgaaagt
    19021 cgcgcgtgta attgctgttt gttcaattgc catctcatga tcaccctgtt acgcataaat
    19081 aaatgtgaaa ttataatcac aaaatatgca taaaaaatca ttgaatggca atcagaaatc
    19141 agcgatgcag gaaattagcc agcagttgat gtccttgttc gctaagaata ctttctggat
    19201 ggaactgcac accttccaga tcccactggc gatggcgaat ccccataatc tcgcgggttt
    19261 cgctccaggc cgtcacgtca aagcacgcag gtaatgagtc aggttccacc accagcgaat
    19321 gatagcgtgt cacagtaagt ggatttgcca gcccccgaaa tacgccctca ccgctatgtg
    19381 ttatcggcga ggttttgccg tgcatgacct ttgcagcgcg cacaacttta ccgccaaatg
    19441 cctgcgccat tgcctgatga ccgaggcaga cgccaagaat cggcaagcgc ccggcatagt
    19501 ggcgaataac gtcaagggag atcccggctt catctggcgt acaggggccg ggtgagatga
    19561 caattttttg tggtttaagg gcgtcgatat ccgccagcgt caacgcatcg ttgcgcttaa
    19621 ccagcacatc cgcccccagt tcacaaaagt actggtagag gttccaggta aaagaatcgt
    19681 agttatctat aagcaggatc atggcggctc cggtacgaaa gaaccgcgct attttactca
    19741 gattccccgg cttcgcttac cactttgcta aatatcatct gcagtgcggt gagatccccc
    19801 attgccccac tctgatttgc ctgattccag gcctcttttt cgataccctg ccagctcagt
    19861 tgatatccgg catgaatcgc cagttgctcg aagaagatcc gctgtgccag accacttccc
    19921 acccggaagg gatgcagcac gttgatttca caatagtaat gcgccagccg ctcgacgaac
    19981 ttcgctttct ccaggccaac cagataacct tcttcctcca gatcctgcat cagggcattg
    20041 ccctcttttt cgatataagc aaagtggcag aacggcgtat cgccctgata aatatcaact
    20101 tcacgcagtt gccctgccca gtcgaaaata tcctgataca gctggcgatg gatagtgcgc
    20161 aaatgcggta aaccgcgcac cagcggacca agctcaatgg tcgcagcacg cagcgccgtc
    20221 atttcgtaag cggcctgttc cagccgctgc tgctggcgga tgttcagccg gttacgcatg
    20281 atatcaaggc ctggataaag atacggatcg cgcccttcgc cgaatttatc gctcatagtg
    20341 cctcctcagc tcttcaaggc gcgctaaagc ctctgcggca gtaagagtga ctaaaggcat
    20401 ctcgacgcct tcaagacggc gactggcctg aaaattacga ttacgctgaa gctcccagag
    20461 acgggacttt tgcttatcgg tgagtttctt cacttgatgc ctccctgaac gtgtccattt
    20521 gccacaagta taagcggcaa atccgggtta cgccgggaga gcagaagcgc gggcaagatt
    20581 gcccgcgcga gaaatcatta cggcaggact ttagcggaaa ggataactac cggttttgac
    20641 ggcacattct ggtacggacc aacgtcatga gtcggaacct gggaaatctt atcggcaacg
    20701 tccatgcctt tcaccacttt accaaatacc gcgtaaccga aatcacgctg accatggtca
    20761 aggaaggcgt tatcggcaac gttgataaag aactggctgg tggcgctgtc tttgtcagcg
    20821 gtacgcgcca tcgcgatggt gccacgcgtg ttgcgcaggc cgttatcggc ttcatttttg
    20881 attggcgggt ttggtttttt ctgctgcatc tgctcggtga aaccgccgcc ctgaatcata
    20941 aagccaggaa tgacgcggtg aaaggtagtg ttgttataaa aaccgctgtt cacataatcg
    21001 acaaagtttt gcacagacac tggcgctttt tgtttatcca gctccagttc gatgttacca
    21061 gctgaggttg tcaacaatac gtgcgggtcc cctttcgctg ccattgctgc gggagaaaga
    21121 gcagaaagag cgaaaacagc agccatcgcc gccagggtcg atttgaacat gagatttcct
    21181 taacaaacag agcagaaaaa aagcaagtga agagattcta aatagccttc aagaacaagg
    21241 ccatcccttt acctaatttt acgtatctga aatatatgta acctgacgat cactttttat
    21301 tccgtgatca aaatcacctc ttaaaatgca atttagcaac cgattgcaat aaaacattta
    21361 aacagatcac aaaatcacct aaaatcgccc gctcgcggca tatcccgcta tgccatttta
    21421 tttttgaacg caggccaatc atgactaaca gcaatcgcat caagctcaca tggattagct
    21481 ttctctccta cgcactgact ggtgcgttgg ttattgtcac cgggatggtg atgggaaata
    21541 tcgccgatta tttcaatctg cctgtttcca gtatgagtaa caccttcacc ttcctcaacg
    21601 ccggcatttt aatctctatc ttcctcaacg cctggctgat ggaaatcgtc ccgttgaaaa
    21661 cgcagttacg ttttggcttt ctcctgatgg tgctggcggt tgccggtttg atgttcagcc
    21721 acagcctggc gctgttctcg gcggcgatgt tcattctcgg agtggtcagc ggcatcacca
    21781 tgtcgattgg tacattcctg gtaacacaaa tgtatgaagg gcgtcagcgc ggttcccgcc
    21841 tgttatttac cgactccttc ttcagtatgg ctgggatgat tttcccaatg atcgccgcgt
    21901 ttctactggc gcgcagcatt gagtggtact gggtttatgc ctgcatcggg ctggtgtatg
    21961 tcgctatttt tattctgacc ttcggctgtg agttcccggc actgggcaaa catgcaccaa
    22021 aaacggatgc tccggtagcg aaagaaaagt gggggatcgg cgtactgttt ctctctgttg
    22081 cggcgctgtg ctacatcctc ggtcagttag gttttatctc ctgggtgcct gagtatgcca
    22141 aaggcctggg catgagcctg aacgacgcgg gcacgctggt gagtaacttc tggatgtcat
    22201 atatggtcgg catgtgggcg ttcagcttta ttcttcgctt ctttgatttg caacgcattc
    22261 tgaccgtact ggctggtctg gctgcgattc tgatgtacgt ctttaacacc ggaacaccgg
    22321 cacatatggc gtggtcaatt ctcgccctgg gcttcttctc cagcgcgatc tataccacca
    22381 tcatcacttt gggttcacag cagaccaaag taccgtcgcc aaaactggtt aactttgtcc
    22441 tgacctgcgg gaccatcggt actatgttga cctttgtggt taccggcccg attgttgaac
    22501 atagcggtcc gcaggcggca ctgcttacag caaacggtct gtacgctgtc gtctttgtga
    22561 tgtgcttcct gttaggtttc gtcagccgtc accgtcagca taacaccctg acctctcatt
    22621 aattgctcat gccggacggc actatcgtcg tccggccttt tcctctcttc ccccgctacg
    22681 tgcatctatt tctataaacc cgttcatttt gtccattttt tgcacaaaca tgaaatgtca
    22741 gacaattccg tgacttaaga aaatttatac aaatcagcaa tatacccctt aaggagtata
    22801 taaaggtgaa tttgatttac atcaataagc ggggttgctg aatcgttaag gtaggcggta
    22861 atagaaaaga aatcgaggca aaaatgagca aagtcagact cgcaattatc ggtaacggta
    22921 tggtcggcca tcgctttatc gaagatcttc ttgataaatc tgatgcggcc aactttgata
    22981 ttaccgtttt ctgtgaagaa ccgcgcatcg cttatgaccg cgtacacctc tcgtcttact
    23041 tctctcacca caccgccgaa gagctgtcgc tggtgcgcga aggcttctac gagaaacacg
    23101 gcatcaaagt tctggtcggc gaacgtgcta tcaccatcaa ccgtcaggag aaggtgattc
    23161 actccagcgc cgggcgcacc gttttttatg acaagctgat catggcgacc ggttcctacc
    23221 cgtggatccc gccaatcaaa ggttctgata ctcaggactg ctttgtctat cgcaccattg
    23281 aagacctcaa cgccattgaa tcctgcgccc gtcgcagcaa acgtggtgcc gttgttggcg
    23341 gcggcctgtt aggtctggaa gccgctggcg cgctgaaaaa cttaggtatt gaaacccacg
    23401 ttatcgaatt tgcccctatg ctgatggcgg aacagcttga tcagatgggt ggcgagcagc
    23461 tgcgtcgcaa aatcgaaagt atgggcgtgc gcgttcacac cagcaaaaac acccttgaga
    23521 ttgtgcagga aggtgtcgaa gcgcgtaaaa ccatgcgttt tgccgacggc agcgaactgg
    23581 aagtcgactt tatcgtcttc tctaccggta tccgtccgcg cgataagctg gcaacccagt
    23641 gtggtctgga cgttgctccg cgtgggggta tcgtcattaa tgattcctgc cagacttccg
    23701 atccggatat ctacgccatc ggtgaatgcg caagctggaa caaccgtgta tttggcctgg
    23761 tagcaccagg ctacaaaatg gcgcaggtcg ccgttgacca tattctcggt agcgaaaacg
    23821 cctttgaagg tgctgacctt agcgccaagc tgaaactgct gggcgtagac gtaggcggta
    23881 ttggtgatgc gcacggtcgc acaccaggcg cacgtagcta cgtttacctc gacgaaagca
    23941 aagagatcta caaacgcctt attgtcagcg aagacaacaa aaccctgctc ggtgcggtac
    24001 tggtgggcga taccagcgac tacggcaacc tgctgcaact ggtgctgaat gctatcgagc
    24061 tgccggaaaa cccggattct ctgattctgc cggcgcactc gggtagcggt aagccgtcta
    24121 tcggtgttga taaactgccg gacagcgcgc aaatctgctc ctgcttcgac gtcaccaaag
    24181 gtgatctgat tgctgccatc aacaaaggct gtcacaccgt tgcggcgctg aaagccgaaa
    24241 ccaaagcggg tactggctgc ggtggctgta tcccgctggt cactcaggta ctgaacgcgg
    24301 aactggcgaa acagggcatc gaagttaaca acaacctgtg cgaacacttt gcttattcgc
    24361 gtcaggaact gttccatttg atccgcgttg aaggcattaa aaccttcgaa gaactgctgg
    24421 cgaaacacgg caaaggttac ggttgtgaag tttgtaaacc gaccgtcggt tcgttgctgg
    24481 cctcctgctg gaacgaatac attctgaagc cggaacatac tccgctgcag gattctaacg
    24541 acaacttcct cgctaacatc cagaaagacg gcacctactc ggtgatcccg cgctctccgg
    24601 gcggtgaaat caccccggaa gggctgatgg cggtaggtcg tatcgcgcgt gaatttaatc
    24661 tctacaccaa gatcactggc tcccagcgtc tggcgatgtt tggcgcacag aaagacgatc
    24721 tgccggagat ctggcgtcag ctgattgaag ccggcttcga aaccggtcat gcctatgcga
    24781 aagcactgcg tatggctaaa acctgcgtgg gtagcacctg gtgccgctac ggcgtgggcg
    24841 acagcgtggg cctcggcgtg gaactggaaa accgctacaa aggcatccgt acgccgcaca
    24901 aaatgaagtt cggtgtctcc ggctgtaccc gtgaatgttc agaagcccag ggtaaagacg
    24961 tgggtattat cgccaccgaa aaaggctgga acctgtatgt ttgcggtaac ggcggcatga
    25021 aaccgcgtca tgcggatctg ctggcggcgg atatcgatcg cgaaacgctg atcaaatatc
    25081 tcgaccgctt catgatgttc tacatccgta ccgccgacaa actgacgcgt accgcgccgt
    25141 ggttagaaaa cctcgaaggc ggcatcgatt acctgaaagc ggtgatcatt gacgacaaac
    25201 tggggctgaa cgcacatctg gaagaagaga tggcgcgcct gcgtgaagcg gtagtgtgtg
    25261 agtggactga aacggtcaat acgccgtctg cgcagactcg cttcaaacac ttcatcaaca
    25321 gcgacaagcg tgacccgaac gtgcagatgg tgccagagcg cgaacagcac cgtccggcaa
    25381 cgccgtatga acgtatccca gtaactctgg tggaggacaa cgcatgagcc agtggaaaga
    25441 catctgcaaa atcgatgaca tcctgcctga aaccggcgtc tgcgcgctgt taggtgacga
    25501 gcaggtcgca attttccgcc cgtatcacag cgaccaggtg tttgcgatca gcaacatcga
    25561 cccgttcttc gagtccagcg tgctgtcacg cggactgatt gcggaacacc agggcgagct
    25621 gtgggtcgcc agcccgctga aaaaacagcg ttttcgctta agcgacggct tgtgcatgga
    25681 agacgaacag ttttccgtca agcattacga agcgcgagtg aaagacggcg tggtacagct
    25741 acgcggttaa tgttttaacg ggaggcgcaa tgcctcccct ttttgcattg tcctgtaatg
    25801 accttcggta tattgcagga tattttttaa actttttgtt ttattttttg tttttatttt
    25861 ttaaaggata atcaaatgtt tacagacact attaataagt gtgcggctaa tgctgcgcgc
    25921 attgcacgcc tgtcggcaaa taacccgctc ggcttttggg tcagctccgc catggcgggc
    25981 gcgtatgtgg gtcttgggat catcctgatt ttcacgctcg gtaatttgct cgatccatcc
    26041 gtacgccctc tggtgatggg cgcgaccttt ggtatcgctt taacgctggt gattatcgcc
    26101 ggttctgaac tgttcaccgg acacaccatg ttcctcacct ttggggtgaa agcgggcacc
    26161 atcagccacg ggcaaatgtg ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt
    26221 tccgtctttg ttgccatgct ctatagctgg ggcggtggta gcctgctgcc ggtagatacc
    26281 agcatcgttc actccgtcgc attggctaaa accactgcac cggcaatggt actgttcttc
    26341 aaaggtgcat tgtgtaactg gctggtttgt ctggcaatct ggatggcgct gcgtactgaa
    26401 ggggcggcga aatttatcgc tatctggtgg tgtctgctgg catttattgc atccggctat
    26461 gagcactcca tcgctaacat gacgctattc gcgctctcct ggttcggcaa tcacagcgaa
    26521 gcctacacgc tggcgggtat tggtcataac ctgctgtggg tgacgctggg taatacttta
    26581 tcgggcgccg tattcatggg attgggttat tggtatgcta cgccgaaagc gaatcgtccg
    26641 gttgcggaca aatttaatca aactgaaacg gctgccggtt aattactaag gggtttttac
    26701 gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt
    26761 ggtgatgtcg cggaacgcaa agcaaggttg ctgttagacg caggcgctcg cttaacggtg
    26821 aatgcattag cgtttattcc acagttcacc gcatgggcag atgcaggcat gttaaccctc
    26881 gtcgaagggc catttgatga aagccttctc gacacctgct ggctggcgat tgcagcgacg
    26941 gatgatgacg cgcttaacca gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt
    27001 aacgtggtcg atgcgccgaa agctgccagc ttcattatgc cgtcgattat tgaccgctca
    27061 ccgctgatgg tggcggtttc ctctggcggt acttctccgg ttctggcgcg cctgttgcgc
    27121 gaaaaactcg aatcactgct gccgctgcat ctgggccagg tagcgaaata cgccgggcag
    27181 ttacgcggtc gggtaaaaca acagttcgcc acgatgggtg aacgccgccg cttctgggaa
    27241 aaactattcg ttaacgaccg tctggcgcag tcgctggcaa acaacgatca gaaagccatt
    27301 actgaaacga ccgaacagtt aatcaatgaa ccgctcgacc atcgcggtga agtggtgctg
    27361 gttggtgcag gtccgggcga tgccgggctg ctgacactga aaggactgca acaaattcag
    27421 caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa tctggtacgc
    27481 cgcgatgctg atcgcgtttt cgtcggcaaa cgcgcgggat accactgcgt accgcaggaa
    27541 gagattaacc agatcctgct gcgggaagcg caaaaaggca aacgcgtggt gcgtctgaaa
    27601 ggcggcgatc cgtttatttt tggccgtggt ggcgaagagc tggaaacact gtgtaacgcg
    27661 ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc tgcctattca
    27721 ggtattccgc tcacgcatcg cgattatgcc cagagcgtac gcttaattac cggacactta
    27781 aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag aaaaacagac gctggtgttt
    27841 tatatggggc tgaatcaggc tgcgactatt cagcaaaagc tgattgaaca cggtatgcct
    27901 ggcgaaatgc cggtagcaat tgtcgaaaac ggaacagctg tcacgcagcg cgtgattgac
    27961 ggtacgctca cacaactggg cgaactggcg cagcaaatga acagtccatc gctaattatt
    28021 attggtcggg ttgttggcct gcgcgataaa ctgaactggt tctccaacca ttaatttaat
    28081 cagggccaga gaattctggc ctttttcgtt tacaactctc tttatatatt cagcagataa
    28141 aaatataact cattaacaaa aatcatttgt ttttgccccg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cactcactga cgccgattaa atattcctgt atcgttctta
    28261 atgagcagtc tacttatatt catataatca atgaatatta atcaatatat aatacatagg
    28321 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcggtactg gctacgttaa
    28381 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28441 acccagcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28501 ctgacagaaa tcagctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28561 gtatttgttt ctatatcctt cttgctcccc acccgtaaaa atattttcac tttaatttca
    28621 attgaataac cacattcatc agccacagcg cgatcctgtg cgaaattatg tgatcttcct
    28681 ccacattaca taacatcata tgttgttata ttcatcatgc attgtcatgt taccttttaa
    28741 atgactgcaa actctcccct acaacgtgtt ggacaagaaa aaggtatcgc tatgggaagc
    28801 caggaactcc aacgcaagct cggattttgg gccgttcttg caatcgccgt cgggacaacc
    28861 gtcggctccg gtatttttgt atctgtgggt gaagtggcaa aagcggcggg cacgccgtgg
    28921 ctgacggtgc tcgcatttgt catcggcggg ttaattgtga tcccgcaaat gtgcgtctat
    28981 gcggaactat ccaccgctta tccggaaaat ggcgcagatt atgtttatct gaaaaatgcc
    29041 ggaagccgac cgctggcttt cctctccggc tgggccagct tctgggccaa cgatgcgccg
    29101 tcattgtcga ttatggcgct ggcgattgtc agcaatcttg gctttttaac gcctatcgat
    29161 ccgttgctcg gtaaatttat cgccgccgga ttaattatcg cctttatgct gctgcacctg
    29221 cgctccgttg aaggtggcgc agcgtttcag acgctaatta ccatcgccaa aattatcccg
    29281 ttcactatcg tcattggtct tgggatcttc tggtttaaag cggagaattt tgccgcccct
    29341 accaccactg cgattggcgc aacgggcagc tttatggcgc tactggcggg gatctccgcc
    29401 accagttggt cgtacaccgg catggcctct atctgttata tgaccggcga aattaaaaac
    29461 cccggtaaaa ctatgccacg agcgctaatt ggttcctgtc tgctggttct ggtgctctac
    29521 accctgctgg cgttggtaat ttctggcctg atgcccttcg acaaactcgc caattctgaa
    29581 acgccgattt ccgacgccct gacctggatc ccggcactcg gcagcaccgc cgggatcttc
    29641 gtggctatca cggcgatgat cgtcattctc ggatcgcttt ccagctgcgt gatgtaccag
    29701 ccgcggctgg aatacgcgat ggcgaaagac aatctgttct tcaaatgctt cggccatgtg
    29761 catccaaaat acaacacgcc ggatgtctcc atcatcctgc aaggggcgct ggggatcttc
    29821 ttcatcttcg tttccgatct caccagcctg ctgggttatt tcaccctggt gatgtgtttc
    29881 aaaaataccc tcaccttcgg ctccatcatc tggtgtcgta aacgcgacga ttacaaaccg
    29941 ctgtggcgta ctccggcttt cgggctgatg accaccctcg ccattgcatc aagcctcatt
    30001 ctggtcgcct caacctttgt ctgggcaccg attcccggcc ttatctgcgc cgtcatcgtt
    30061 attgccactg gtctgcctgc ttacgccttc tgggcgaagc gtagccgcca gctcaacgct
    30121 ttgtcgtaat gttatctgga gaaaataaaa tgttggatat tgataaaagc accgtggact
    30181 ttctggtcac cgaaaatatg gttcaggaag tggaaaaagt tctcagccat gacgttccgc
    30241 tggtgcacac catcgtggaa gagatggtga agcgcgacat cgatcgtatt tatttcgttg
    30301 cctgcggatc gccactcaac gcggcgcaaa cggcgaaaca tctggcagat cgcttttccg
    30361 atcttcaggt ttacgccatt tccggctggg agttctgcga taacaccccg tatcgcctcg
    30421 acgatcgttg cgcggtgatt ggcgtttctg actacggtaa aaccgaagag gtaatcaaag
    30481 cgctggagct gggccgggcc tgcggcgcac tcactgcggc gttcaccaaa cgcgcggaca
    30541 gcccgattac ctcggcggcg gaatttagca ttgattatca ggccgactgt atctgggaaa
    30601 ttcatctgct gctctgctac agcgtggtgc tggagatgat cacccgcctc gcgccgcacg
    30661 cggaaatcgg caagatcaaa aacgatctca agcagctgcc aaatgcgctc ggtcatctgg
    30721 tacgcacctg ggaagagaaa ggtcgccagc tcggtgaaca ggccagtcag tggccgatga
    30781 tttacacggt tgctgcaggc ccgctgcgcc cgctgggtta caaagaaggc atcgtgacgc
    30841 tgatggaatt tacctggacg cacggctgtg tgattgagag cggtgagttc cgtcacggcc
    30901 cgctggagat tgtcgaaccg ggcgttccgt tcctgtttct gctcggcaat gacgaaagtc
    30961 gtcacaccac cgaacgcgcc attaactttg ttaaacagcg tactgacaac gtgatcgtca
    31021 tcgattacgc cgaaatttcg caagggctgc acccgtggct ggcaccgttc ctgatgttcg
    31081 tgccaatgga gtggctctgc tactacctgt ctatttacaa agatcacaac ccggatgaac
    31141 gccgctatta cggtggtctg gtggaatatt aatcctctct cccggcccgg caacgggccg
    31201 gatttacgca aggagttacc cgatgaaaac aggtatgttt acctgcggcc accagcggct
    31261 gcctattgaa cacgcattcc gtgatgcaag cgagctgggt tacgatggca tcgaaatttg
    31321 gggcggccgc ccgcacgcgt tcgcgccgga cttaaaagcg ggcggcatca aacaaatcaa
    31381 ggcgctggcg cagacgtatc agatgccgat tatcggttac acgccagaaa ccaacggcta
    31441 tccgtataac atgatgctgg gcgatgaaca tatgcgtcgt gaaagcctgg acatgatcaa
    31501 gctggcgatg gatatggcaa aagagatgaa cgcgggttat acgctgattt ctgcggccca
    31561 cgcgggctat ctcacgccgc ctaacgttat ctgggggcgg ctggcagaga acttaagcga
    31621 actgtgtgag tacgcggaaa acatcggcat ggatctgatc ctcgaaccgt taacgccgta
    31681 tgaatcgaac gtcgtgtgta atgctaatga tgtgcttcat gcgctggcgc tggtgccttc
    31741 gccgcgcctg ttcagcatgg tcgacatttg cgcgccgtat gtccaggcgg aaccggtgat
    31801 gagttatttc gacaaactgg gcgataaatt acgtcatctg catattgtcg acagcgacgg
    31861 ggccagcgac acgcattaca ttcccggcga aggcaaaatg ccgctgcggg aactgatgcg
    31921 cgatattatt gatcggggct atgagggata ctgcacagtg gaactggtaa cgatgtatat
    31981 gaacgagccc agactttacg ccagccaggc gctggaacgc tttcgcgcgc tgctgccgga
    32041 ggatgagtga tgaaaaccct ggcgacaatc ggcgataact gcgtcgatat ctacccgcaa
    32101 ctgaataaag cgttttctgg cggtaatgcg gtgaacgtgg cggtgtactg cactcgctac
    32161 ggtataaagc cgggatgcat tacctgggtg ggtgacgatg actacggcac aaagcttaag
    32221 caggatctcg cccgcatggg cgtcgatatc agccatgtcc atacgaaaca cggcgttacc
    32281 gcacaaactc aggtggaact gcacgacaat gatcgcgttt ttggcgacta caccgaaggc
    32341 gtgatggccg actttgccct gagtgaagag gattacgcct ggctggcgca gtatgacatt
    32401 gtgcacgcgg caatctgggg acatgcggaa gacgcattcc cacagctgca cgctgcgggc
    32461 aaacttaccg ctttcgactt ctccgacaag tgggacagcc cgctctggca gacactggtg
    32521 ccgcatctcg attttgcctt tgcctctgca ccgcaagaag acgaagcgct gcgtctgaag
    32581 atgaaggcga ttgttgcccg tggcgcagga acagtgattg tcacgctggg cgaaaacggc
    32641 agcattgcct gggatggcgc gcagttctgg cgtcaggctc ctgaactggt gacggttatc
    32701 gacaccatgg gtgccggaga ttcgttcatt gccggattcc tttgcggctg gtctgcgggg
    32761 atgacattac cgcaggcgat ggcgcaggga acggcgtgcg cggcgaaaac cattcagtac
    32821 cacggtgcct ggtaggtata acgttggcgt gagcatcttc acgccaacgt gctgttactt
    32881 gccggaaaac gaccctataa tccgagtaat tcattcttta tttcagggtc gattatgtca
    32941 gctacggacc gctactctca tcaactcctc tacgctaccg tccgccagcg gctgctggat
    33001 gatatcgcgc agggggttta ccaggccggg caacagatcc ctaccgaaaa cgagctttgt
    33061 acgcaatata acgtcagccg cattactatt cgcaaagcta tcagcgactt agtggcagac
    33121 ggcgtactga tccgctggca aggcaaaggc acctttgtac aaagccagaa ggtcgaaaac
    33181 gccctgctga ccgtcagtgg ttttaccgat tttggcgtct cacaaggcaa ggcgacgaaa
    33241 gagaaagtga tcgagcagga acgggtcagc gccgcgccgt tttgcgaaaa gctgaacatc
    33301 cctggcaaca gcgaagtgtt ccatctttgc cgggtgatgt atctcgataa agagccgcta
    33361 tttattgata gttcgtggat cccgctgtcg cgttatcctg actttgatga gatttacgtc
    33421 gaaggaagct ccacctatca gttatttcag gagcgttttg acacgcgagt ggtcagcgac
    33481 aaaaagacca tcgatatctt tgccgccacc cgcccgcagg cgaaatggct gaaatgcgaa
    33541 ctgggcgaac cgttgtttcg catcagcaaa atcgcctttg atcagaatga caaaccggtg
    33601 cacgtctccg aactcttctg ccgcgccaac cgcatcacct taacgattga taataaaaga
    33661 cattaaccgt aggccggata agatgcacca gcatcgcatc cggcgatgcc ggcgcactga
    33721 attttacatc ccgcacgttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
    33781 tccgagcgtt ttgatattac ggcttcgcca caaaaccaat cgcttcatac accgctttta
    33841 gcgtacggga agcgtgcgcg ctggcttttt ccgcgccatc tttcatcacc tgttgcagga
    33901 aggcttcatc gttgcggaaa cggtgatagc gttcctgcaa ttcagtcagc ataccggaaa
    33961 cggcatcagc cacttcacct ttcagatgac catacatctt gccttcgaac tgtttttcca
    34021 gttctgggat gctctggccc gttaccgcag aaaggatatc cagcaggttg gaaacgcccg
    34081 ctttgttctg cacatcgtag cgaactaccg gcggctcgtc ggagtcggtg accgcgcgtt
    34141 tgattttctt cactaccgat ttcggatctt ccagcaggcc aataacgtta ttgcggttat
    34201 cgtcagactt ggacatcttc ttggtcggct ccagcagcga cattacgcgc gcgccagatt
    34261 tcggaataaa cggctccggc accttaaaga tctcgccgta cagcgcgttg aaacgctgag
    34321 cgatatcgcg gctcagttcg aggtgctgtt tctggtcttc acccaccggt accagattag
    34381 tttgatacag caggatgtcc gcagccatca gcaccggata gtcaaacaga ccagcgttga
    34441 tgttctcggc ataacgcgca gatttatctt taaactgcgt catgcggctc agctcgccga
    34501 agtaggtata gcagttcagt gcccagccta actgcgcatg ttccggtacg tgggactgaa
    34561 caaaaatggt gcttttctca ggatcgatac cacaagccag atacaaggcc agcgtatcca
    34621 gcgtcgcttt acgcagcttc tgtgcatcct ggcgcacggt gatcgcgtgt tggtcaacga
    34681 tacagtaaat gcaatggtag tcatcctgca tgtttaccca ctgacgcagc gcacccatgt
    34741 agttaccaat ggtcaattca cctgagggct gtgcgccact aaaaacgatg ggcttagtca
    34801 tttttcgatt cctgattttc gctatgcgga agccctaatg cgggcagaag gtcatttata
    34861 gactgataaa ttacatcagg ctggctgaga tcgatagcct cgccgtagtt atatccgtag
    34921 gttaagccaa ctgatgggca acctgccgct tttgctgcct gaatatcatt gcgtgagtcg
    34981 ccaacaaaca gcatctgttg tggggcaatt cccatccgct cagccaccag taacagcggg
    35041 tccggatgcg gttttttgtt ttgcacatca tcaccaccaa tcaccacgct gaagtatttg
    35101 gcgatatcta aggcttcgag cagcggcgcg acgaacggcg tcggtttgtt ggtgaccagg
    35161 cctagcggca ggcctttagc ctgcaacgcg cccaacgtat cggcaacgtg cgggaacaaa
    35221 aacgtcccct cttcggcaac ctcgccataa tagcgatcga acagtttacg cagaatacgt
    35281 acctgttctt ctgccggaat gtcgtcatca acgggcggtt tacccattgt tttacgcaga
    35341 gtcgcacgtt cctgacgcgc ccaggtcaat gcgcgctcca tcagaacatc tgcgccgtta
    35401 ccaatccagg taataacgcg ttcttcacct gcgacgggta actccagcgc atacagcgcc
    35461 atatctaccg cagcagcaag accgggagca ctgtccacca gcgtaccgtc gagatcaaaa
    35521 gcgacgccgc gaatatcttc aaacttactc atgacttacc tttgccagtt cactgcgcat
    35581 ttcatcaatg acttttttgt agtctggctg gtcgaagatt gccgaaccgg caacgaacat
    35641 atccgcgccc gccgcggcga tttcgccaat gttgttcacc ttcacgcctc cgtccacttc
    35701 cagacgaatg tcaaagccag actcgtcgat acgacgacgt acttcgcgca gtttatccag
    35761 tgtttgagga atgaaagact gaccgccgaa accaggattg acggacatca gcaggatcac
    35821 atccagctta tccatcacgt aatccagata gctcagaggt gtcgccgggt taaataccag
    35881 acccgcttta cagccatttt ctttaatcag ttgcagcgtg cggtcaacat gctcggaggc
    35941 ttctggatga aaggtaatga tgctggcacc agcggcagcg aaatcaggca caatgcgatc
    36001 gacaggtttc accatcaggt gtacgtcgat aggggcggta atgccgtagt tacgcaagga
    36061 tttcagcacc attggcccaa tcgtcagatt gggaacatag tggttatcca tgacgtcaaa
    36121 atgcacgaca tcagcgccag ctgccagggc ttttgcggta tcttcaccca ggcgggcaaa
    36181 atcagccgac agaattgagg gggcaatcaa atactgtttc atccgcttct ccttgagaat
    36241 tattttttcg cgggtgaaac gactcctggt ttgtacaaag ccagcagttc gtccaccttt
    36301 ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt tgacgacatg caattttgcg
    36361 cgctgatacc actcacgcgt taacatcgta tcgtgattgg agatcagcac tggaatatgg
    36421 cgatcaacca gaccttcggc gatctccgcc agatgcgctt gttgttcaag cgtaaaactg
    36481 tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg gtgcataagg cggatcgcaa
    36541 tagacgacag atgcatcatc tgcgcgcgcc atgctatcgg cgtaagactc acaatagaaa
    36601 aaggcattct gcgctttttc agcgaagtga tacaactctg cttccgggaa atagggtttt
    36661 ttgtagcggc cgaacggcac gttaaactca ccgcgcagat tgtaacgaca caggccgttg
    36721 taaccgtagc ggttcaaata taaaaacagt accgcccgac ggaacggatc ctggcttttg
    36781 ttgaactctt cgcggaactg atagtaaacc tcggcgcaat ttgtttcggg aacaaacagc
    36841 tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca caatgttata gagactgatc
    36901 aggtcgctat tgatatcggc aaggatataa cgagaaaagt cggtgttgag aaacaccgac
    36961 ccggcaccta caaaaggctc aaccagacat tcgcccttgg gcaaatgccg tttaatatca
    37021 tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag cgcgattttt cttcatgctg
    37081 actaactaat tacaccttct ccggctgtgg agaaagctcc gacagcatcc agcgcttcag
    37141 gcaactcccg caacgataaa ccattgcggg agataacctt gattacttca gatcggcctg
    37201 tacctgacgc agcggtttcg cccacgggtt tttggcctgg acatctgctg gcaatgtaga
    37261 taccgctttt ttcgcctctt ctttcgaagc gtacacgcca gaaaccagga cataccacgg
    37321 ctgaccatta cgcgtcgttt catagacaac gtagtttttc agattctctt tcttcgccca
    37381 accgttcagg ttgtcgtagt tagaggaact gctcagctgc agagtgtaat ggctggacgg
    37441 tgccgatttc aacgaaccaa cattacctgc ggtcttccct ccagcggctg gtgttgccgt
    37501 ggtttgcgcc ggggatgccg tctgtactgg agccgtagtc gccgtctctt ttggtgctgg
    37561 cgtagaagtc gcagccggtg ccttcgtgct cgccacagga gcagctggtt cagtacgctt
    37621 cggcgtctgt gctaccggct tcggttccgt tttcacggtt gcttgcggtt ttttcggttc
    37681 aatcaccgcc tgctgacgag ctggacgcgt agtggccgga cgttcagcag tttgcgtttt
    37741 cgccgtgtca cgcgatgcat tgccattgcg tactggcgcg acagttgctg gttcagtcgg
    37801 caatgtagaa ttgaccgcca cattgttcaa ctgttgctga ttttgtggct gggtcagcgc
    37861 attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgccg ccggggtttg
    37921 cccttgagtc ggcgtagaag agatcggtgg cagagaaaca tcctgctgag tattttccgc
    37981 agacgtggtt cccggcgctg gctgcacgcc attcgcctga tcggtcgcat tgccagcaag
    38041 atcaatactc ttctcgccag acgcggtttg atcgctggaa gaggtcgagg gggcttttag
    38101 cgcagaaccg ataccgatga tcaacagcag tagaaccaga atgccgacgc ccatcatcat
    38161 atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgcggac gacgctctac
    38221 gcgctcttca tccacggttt catcttcgga ttcgtcaatt tcttcttcga tttccggttc
    38281 ctcattgcgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat caagttcaat
    38341 atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt cagaagattg
    38401 acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt ctggtttgaa
    38461 ttcatccatt taacacccca ctaaaaggtt aatgcttacc acgttgcaat taacctgaag
    38521 ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat tgacaatcgg
    38581 caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc ttaccaattg
    38641 ccaacggaag aattaagcgc atctctcccg caaggacttt cttgtcacgc agcatatgcg
    38701 gtaaatacgc ctgcgcggac atttcgcgcg gcccattgac cggtaaccca gcccgcttga
    38761 gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt tccgacgtcc
    38821 gcgccgccat caccataccc gcagcgaccg cttcaccatg taaccaattg ccatacccca
    38881 tttcagcttc aatggcatga ccaaaggtgt gtcccagatt cagtaaagca cgtaacccgg
    38941 tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg cgaatacagt
    39001 acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc agccagttga
    39061 aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgccagcccc gacgctaact
    39121 cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca ggctggtaga
    39181 acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg ccaacggagg
    39241 aatcgacctg cgacagtaac gtcgtcggga cttgaatgaa acgaacaccg cgctgataac
    39301 tcgccgccgc gaagccggtc agatcgccca ctacgccgcc gccaagcgcc accagcgtag
    39361 tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagt acagctaggc
    39421 ttttatactg ctcgccgtca ggaaggataa cgctatcgac gttaacaccc gcctgttcaa
    39481 gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgttggtg accaacatga
    39541 cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa ccagatgcga
    39601 tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata acgcgacatc
    39661 caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt tgctttccag
    39721 catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac gaatggtcac
    39781 gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca gaacttcacg
    39841 tggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc gtgcaagttg
    39901 cttttcgatg gtcgtttcaa gataaacgac aacgccacga gcggaaagac ggttacgcgt
    39961 ttcacgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt tctcggtcaa
    40021 ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta aatcgaaaac
    40081 ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt aaaattccat
    40141 attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca taggcccaac
    40201 cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat tcgttaatga
    40261 taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat aagatattgc
    40321 gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga ataaatcacc
    40381 catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg ctctggcgat
    40441 aaatcaccgg gtaagattag cgtaaaaaag acagcaaaat gccgcctgaa tgataaatca
    40501 tcatcatgga acgcaggcag cgaagagcac caactctcaa atcggtactc cttgtatgct
    40561 aaatacctgc gcgcgtcaaa tagatgaaac acgttcagcg taaaaacatt accgctttta
    40621 cggctgttta ctcactggaa accagtcgtg gcgtgataaa caccactaac tcgcgtcgtt
    40681 catcttcttt tccgtcatga cgaaataatt gcccgaacca gggaatgtcg ccaagcaacg
    40741 gcacgctatc ctgacccgat ttatttttac gggtaaaaat gccgcccagc gccaacgttt
    40801 ctccgctttt gacctcgacc tgcgtttcga tctcctgctt atcaatcgcc agcacttcgc
    40861 catcggcctg ctgtagcacc tgccccggaa cgttctggct gatgtgtaat ttcagccgga
    40921 tgcgaccttt ttgtaacacc gtgggcgtga cctccatccc caggactgcc tctttaaatt
    40981 ccaccgacgt cgcgccactt tccccgctgg aaacctgata tggaatttcg ctcccctgtt
    41041 taatgctggc aggctgaaga tgtgaggcca gcagacgcgg actggcgata atatccagct
    41101 gctgtttttg ttcgagcgcg gaaagctcaa gatccagcaa acgtccgttg atgcgcccaa
    41161 tgttaaaacc gacatgcgtt gtcgccgtcg ctacggatag gtcgctgcca agcgtggtga
    41221 cttgcccaac gccaccagct tgttgcgcat cggccagcgt ccatttcacg cctaactcac
    41281 gcaaactttt ttcattaatg gtgacaatat gcgccgacag ctcaacctgc ccgaccggca
    41341 gatccatttg cgctacccac tgttcaagcg cgcttaacgc cgttttgtta tctcgcagca
    41401 aaaggcgatt ggtgcgttta tcgacggtca tattcccttt ggcactcagt agcttctccc
    41461 ccgctttcgc cagctctccg gcgtcggcgt attgcagagt aatattgcga ttttccagcg
    41521 gcagatttgc ctgcgcccgc gcctgctccg cttcctgacg ggcgatattg tcattctgcc
    41581 aggcaatgga atgcactgag agaatgttgc cctcctggcg cgttatcagt ccggcgcttt
    41641 tcactacagt ttgtagtgcc tgcttccagg gaacatctgt tagatgtaac gacaccgtac
    41701 cgctgacgtc tggcgacacc accaggttca acttctcctg ttcagccagc gcctgcaaca
    41761 cctgagctac cggaacgtca tccaccatca gcgtcacttt ttgcggcttt gccgcctgta
    41821 cgccgggtat cagcatcaac agtagtgcgg ctatccattg cttcatttgt atctccttgc
    41881 cgttgccaca accattgtgg cggttcgcag tttgtcccgg tacccagcgt tagtacgtct
    41941 ggcgtcagct gtaaaattgt ccagccgttt tccagcacat cgttttgctg cacccgtcgc
    42001 catttctttt gcccgtcttt tattacaccg atgatgcgct cgcctcgccc taccatcccc
    42061 tgatagcgcc actggctaag ttcgctaatc cggcatagat cttccggcgg tttaaaaggg
    42121 tcacgcatac cggttaaaag gcacaatgca atacctgcca acaaccagcg tttaaccctc
    42181 atgcggcgtc tccagttgta gcgtgaacaa aagatcatca ccttccacgc ttaatgaaaa
    42241 acggctcacg ctgacgttgc gctctgccag ccgtgtaaat gccgacggca ctgcttccca
    42301 cagcgttttc aacgccaact cgcctccctg cgcggatgga tgccaggaaa ccagttgcgc
    42361 gccggataac tgaaaatcca gtggcgaaaa gggcagcgtt ttttcttcgc tgaagggagt
    42421 ggtgtcaacc aggcgataca gtgtggccca atgctgatga tgacttgccc gtagtcgaat
    42481 taatgcgtcg cgctcttcat ggtgtgtcga tgacagaaaa atgagcgtaa ctaacatcag
    42541 caaccagaat gcccagcaaa actggcggag gcggggtgat gtggcgaacc accagtcaaa
    42601 gaacatgttc atcgctaacc ttccttgtta actgatactc aaattgccag cgtccctgcg
    42661 catcctgctg cgtggccccc cgctgattga gatgaaaaga agcatcctgg cggagtgacg
    42721 tttctagtgc gtttaacgcg gtaatgcttg ttgtcagccc cttgatctcc agcgttccct
    42781 gctgccagct tatcgttgtc agccaggcgt gctcgggtaa aagcgccgcc agtgcttcca
    42841 gctcagattg ccagtcgcgg gtaaattgtc gctggcgctg ccgttgcaaa cgctgttcgc
    42901 gtaactgctg ttgctccagc aaacgtggtt tcgttatctg taagctgcgg gcgagttgtt
    42961 gttccgcctg aagcaaaacg gcgtctatgc gcgcttcggc gctgcttgtc agacgcagta
    43021 ttagcgttat cccgacggcc agcagcagag gcgcaacgaa catcagcaac cagaaacgaa
    43081 gaaaagcggt ccggcgttgc tgtcgccagg gcaaaaaatt aattggcggg ttcatcagta
    43141 cgccttccca agcgccagcc ccagcgcgat ggcaaagtct ccacctggcg gcggtaacgg
    43201 cggctgacga acagaaacgg cctcccaggg atcaaatccg ccttcgccac atatcgcgac
    43261 gctttcggga tcaacggata acgctgccgc cagctccttc gcgctagtca tccccaccgc
    43321 cagtttgcgc ccccagcgat agcgcgtcgc ccacagccac tgttcgttat cacgccaggc
    43381 cagacattgc tgatgagaag gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg
    43441 ggtgatcgca ctaacatgaa cacgcaacct ttctgccaga gtaagcagcg ttgccagctc
    43501 tttgctttgc gcggcagtca cgttataggc gggactgagt gagtcttcgc tgtaatcgaa
    43561 gcgcagggag tccggatcca tatccagctc gcgggccatc gtcccggaaa gccaggccgt
    43621 ttgctcccgc tcaccgaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact
    43681 ggcgggaaac gccaacatga tgtgatggcg ctgcggcagt tcgcgactcc acggtaacaa
    43741 cgttttagcc agccgctgcg catcaacaat ccgcccatct ttgataatgt cgtgcgccag
    43801 cggcaaccgc caccagcgtt gcaaaaagca ttcttttgcg ccccgcacaa tagcaaccgc
    43861 taccgcttct tgctgttgta aatgcaaacc aatttgccag atcttaaatg ccattgtgat
    43921 gatctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac
    43981 taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt
    44041 tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt
    44101 atggcctata ccgctacatc gagccacaac tgccggatgt ggcgacatta aaagatgttc
    44161 gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg
    44221 agaaacgtcg tattccggtt acgttggatc aaatcccgcc ggagatggtg aaagccttta
    44281 tcgcgacaga agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc
    44341 gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca aggggcgagt accattaccc
    44401 agcagctggc gagaaatttc ttcctcagtc cagaacgcac gctgatgcgt aagattaagg
    44461 aagtcttcct cgcgattcgc attgaacagt tgctgacgaa agacgaaatc ctcgagcttt
    44521 atctgaacaa gatttacctt ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct
    44581 atttcggaaa aacggtcgac caactgacgc tgaacgaaat ggcggtgata gccgggctgc
    44641 cgaaagcgcc ttccaccttc aacccgctct actcgatgga tcgtgccgtc gcgcggcgta
    44701 acgtcgtgct atcgcggatg ctggatgaag ggtatatcac ccaacaacag ttcgatcaga
    44761 cacgcactga ggcgattaac gctaactatc acgcgccgga gattgctttt tctgcgccgt
    44821 acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag
    44881 acggttatcg catttacacc accatcaccc gcaaagtgca gcaggccgca cagcaggcgg
    44941 tacgtaataa cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc
    45001 tgtggaaagt gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc
    45061 tgccaaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct caggaagcga
    45121 cggcaatgct ggcggacggg tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc
    45181 gtccttaccg ttcggatact cagcaaggac cgacgccgcg taaagtgacc gatgttctgc
    45241 aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc
    45301 cggaagtgaa ctcggcactg gtgtcgatca atccgcaaaa cggtgccgtt atggcgttgg
    45361 tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg
    45421 taggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg
    45481 caagtatgtt gaacgatgtg ccaatttctc gctgggatgc aggtgccggt tctgactggc
    45541 agccgaagaa ctcaccaccg cagtatgccg gtccaattcg cttacgtcag gggctgggtc
    45601 agtcgaaaaa cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag
    45661 aatatctgca acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc
    45721 tgggttcagc gtccttcact ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg
    45781 gcggcttcct ggtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga
    45841 ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgtga tattccggtg atttacggtg
    45901 atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg
    45961 agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcattagtgg
    46021 cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga
    46081 ttaagagtgc tttgaacacc aatatctttg gtgagccagg ctggcagggt actggctggc
    46141 gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc actaacagtt
    46201 cgaaagatgc gtggttctcg ggttacggac cgggcgttgt gacctcggtc tggattggct
    46261 ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga
    46321 tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag
    46381 ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc gccgggtatt gtgacggtga
    46441 atatcgatcg cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca
    46501 tcgaaggtac gcagccgaca caacaagcgg tgcacgaggt gggaacgacc attatcgata
    46561 atggcgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagtttgct
    46621 gacaaagtgc acttgtttat gccggatacg ggtgaacgcg ttatccggct aacaaaatcg
    46681 tgaaaactca atatattgca ggaaccccat aagcctgata agcgttgtgc atcaggcaaa
    46741 cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc
    46801 tgacattacg cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg
    46861 gccagcgcac ctgtggtagc ggctctggct catcgccttc cagcgattcc gggtagagat
    46921 cttgcgctac cacgatattc attttgctgg aaaagtaaga cggtgccatg ctgagcttct
    46981 tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg
    47041 cttcgtagac gctttcaccc ggatcaatta atcctttcga aaaacctaat tcgtaggatt
    47101 cagttcccac tgcgtattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca
    47161 tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga
    47221 actccagatc cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca
    47281 gaatggtggg tttttgtaat gatttgctca tcgtgggatc tatgctgtga aatcagcggt
    47341 tattgtgcga tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt
    47401 aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag
    47461 gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc
    47521 ttaatgatgc tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa tcatgtattg
    47581 tgtccggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca
    47641 ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag
    47701 tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta
    47761 aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat
    47821 tacaggtgcc tggcccaacg ggagccagcg cggcaccgat ctctctggcg ctgaatgccg
    47881 aaagcaacaa cgtcatgatg ctgacacacg ctatcacacg ttacggcatc tctaccgacg
    47941 atccgaataa atggcgttac tacctcgatt cggtagaagt ccacctgccc cccttctggg
    48001 aacagtacat caacgatgag aataccgttg aactgattca taccgattcg ctgccgttgg
    48061 ttatttcact caacggtcat acgctgcagg agtacatgca ggaaacgcgc ggctatgcct
    48121 tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc
    48181 tactcaatat tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc
    48241 gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg
    48301 atgtatttgt tccgtggctg gcaggtggcg cgttactgct gctgggcgca ggtctgtggg
    48361 ggctattcgc ccccccggca aaatcctccc tgcgggaaat tcattgtctg cgcggtacac
    48421 cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg
    48481 gtattatcga cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc
    48541 aacaaaccga tatcgatatc tatctcgacc gccacgtagt gcgtcaggga cgatatcttt
    48601 cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg
    48661 ctgcgggctc gctgctggtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc
    48721 tgaaattcac tctctcatgg atgaaaggcg cgcagaccat tgaagccacc agcgtaaaac
    48781 aactggctga cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt
    48841 gtaatattcg cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg
    48901 actgctcgca gatcatctgg aatgacgccc gctcattacc attaccagaa tctgaactgg
    48961 tcaacaaagc gacggcattg actgaagcag ttaatcacca gctgcaccct aaaccggaag
    49021 atgaatctcg cgtcagcgcc tcattacgtt cagcaattca aaaatccggc atggtattac
    49081 ttgatgattt tggcgacatt gtactgaaga cagcagatct gtgttctgcc aaagatgact
    49141 gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg
    49201 taaaacgcgc caacgccggg aaactcgatg gcgtgaatgt gttattacgc ccggtgagtg
    49261 cggaatcact ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg
    49321 cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg
    49381 aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc
    49441 aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg
    49501 ccgagggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat ataggccttc
    49561 atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacactg ctgctactga
    49621 cgatgctggg tagcgccatt tataatggcg tacaggcctg gcggcgttac cagcgtcatc
    49681 gcactcgcat gatgaagatt caggcctatt atgaaagctg cctgaacccg caactgatca
    49741 ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc
    49801 gcaaattgct tcactctgga gatttccctc atgcatatca acattgcctg gcaggacgta
    49861 gataccgttc tgctggatat ggacggcacg ttgctcgacc tcgccttcga taactatttc
    49921 tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg
    49981 atggaatata tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt
    50041 gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg
    50101 cgtgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag
    50161 cagcgaattt tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact tgagcatacc
    50221 ggtctggacg cacaccttga tttattactt tccacccaca catttggtta tccgaaagag
    50281 gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg
    50341 tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc
    50401 ctcggcgtga ctaatcctga ctcagggatt gccgagaaac agtatcaacg ccatccgtca
    50461 ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagaaaa
    50521 acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg
    50581 cgcgctggcc cgtgaaatga ttgagggcgg taaggtgcat tacaacgggc agcgcagcaa
    50641 gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg
    50701 cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct
    50761 gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgctgg cacgtaaact
    50821 taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct
    50881 gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc
    50941 gcaacatgac caattacatc gctatctgtt tgaaaacttt gccgtgcgcg gcgaactggt
    51001 aaccgtttcg gaaaccctgc aacagatcct tgagaatcac gattatccgc agcccgttaa
    51061 aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga
    51121 tggtgatatc accgtacagc tgcaggggga cggtccgatg aatctggcgg ttattaacgg
    51181 taacaataac cagcaaatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc
    51241 cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg
    51301 cgaacgctat cagggcgtgg tgggtctgga aggagatacc ctggcggcct gcctggaaga
    51361 ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga
    51421 cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca
    51481 ggacgacttt gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac
    51541 cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga
    51601 tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa
    51661 aacgctgcct gatgaagaag tcgatagcat cctggcggaa gatggcgaaa ttgacatgca
    51721 ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa
    51781 caacgcgtct ccggcagatc cgcaagttca ttaatgagta tgtccggcag agagggctct
    51841 gccggatttt acgactaata cacctgatgc gctttgtcac tcattcagat tcacatccag
    51901 cctttcacca tccactacaa gatcgcgcca cgctttaggc ttcgttccca gttttccatc
    51961 cttcgcagct gtgagtaatt tttctttcgt tatatcacgc ggaagtaaac tcatcgtaag
    52021 ctcttgccat tctttcttgt caggatcaaa gccatagaca atgtatcttt catcatcaaa
    52081 ggcaaagagg atccgctctg ccctgccatc actattcaga tcttgctcga ccagtacgca
    52141 ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa
    52201 tgtcgcatcc ggtttaccag aaccaggggc aattaacaca ttttcggcta atactttttc
    52261 cgatatctgt tgctgaagat gttgctctcc atctaacgcc atcaacagat cccgcgcacg
    52321 tttcgggtct ttcataaacc cggcatcgct tttcagcgac tcaagcgccg cacgtccata
    52381 gcgaccgctc tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccactctg
    52441 ataacgcgcc atatgactgt tcacgctaat gcgcatactg tccagcaccg gagaattaag
    52501 aagcaccagt attaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac
    52561 aaggggattt tgccctttac gccatacgat actgacaaaa taccccagcg accataccag
    52621 taacaccagc accgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac
    52681 ccgcagccat aacgcccagg cggcgacgaa cacatacagc ggagcaacca gcaaagcggt
    52741 tttaatcagg caacgcaacg gccccgtcca gggaagagac gctttttgcg gatcgcggac
    52801 aatagccatt aagatcaatt gcaaaaaggc cagcgtcaac agcaacccgg cggcggagat
    52861 gtggcgagaa atcgcgctca ggcccgtaaa cggcagggtg atgataaaca tcagggttag
    52921 caatgatact aacggcagca accccgtggc gattaacgtg aacaactttt gaatagagtc
    52981 gattaaacgt gactgtgttc gcgcgaggat caccgccagc gcggtaacca gacctaacgt
    53041 gagatacata aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag
    53101 tttaaacaac tcgtcccaca gtaaaagcac cagccacgtc aggccattgg cgagaaaaat
    53161 caccagtaat ataagtacgt tatgccatac tgactggtaa aaatagcgat agcggctact
    53221 gtcattgcgg atacgaaggc tttgttgtat ccacggtagc aacaacattg ccatcagcag
    53281 cagatagcag ccaaaatccc aaagcgcctt ttcagctctc caggggttca tgccatcagt
    53341 ttgccacttc aaccagccgc tcatccccag cgtggcaata aacaccagcg ccagccatcc
    53401 ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcaac
    53461 ggtcgcaggc acgccataaa caatccaact gtgatttttt ccggcaagcc aggcgatcag
    53521 cagatagcaa accagcccct gaagtaatcc ggtagcaatc atcccccagc gtgttgcggg
    53581 tgaaagttca acgttatcca tgttggttat ccagaatcaa aaggtgggtt aattatcgca
    53641 tccgggcagt agtattttgc ttttttcaga aaataatcaa aaaaagttag cgtggtgaat
    53701 cgatacttta ccggttgaat ttgcatcaat ttcattcagg aatgcgattc cactcacaat
    53761 attcccgcca tataaaccaa gatttaacct tttgagaaca ttttccacac ctaaaatgct
    53821 atttctgcga taatagcaac cgtttcgtga caggaatcac ggagtttttt gtcaaatatg
    53881 aatttctcca gatacgtaaa tctatgagcc ttgtcgcggt taacaccccc aaaaagactt
    53941 tactattcag gcaatacata ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg
    54001 accccgcaag aactcgaggc ttatggtatc agtgacgtac atgatatcgt ttacaaccca
    54061 agctacgacc tgctgtatca ggaagagctc gatccgagcc tgacaggtta tgagcgcggg
    54121 gtgttaacta atctgggtgc cgttgccgtc gataccggga tcttcaccgg tcgttcacca
    54181 aaagataagt atatcgtccg tgacgatacc actcgcgata ctttctggtg ggcagacaaa
    54241 ggcaaaggta agaacgataa caaacctctc tctccggaaa cctggcaaca tctgaaaggc
    54301 ctggtgacca aacagctttc cggcaaacgt ctgttcgttg tcgacgcctt ctgtggtgcg
    54361 aacccggata ctcgtctttc cgtccgtttc atcaccgaag tggcctggca ggcgcatttt
    54421 gtcaaaaaca tgtttattcg cccgagcgat gaagagttgg caggtttcaa accagacttt
    54481 atcgttatga acggcgcgaa gtgcactaac ccgcagtgga aagaacaggg tctgaattcc
    54541 gaaaacttcg tggcttttaa cctgaccgag cgcatgcagc tgattggcgg cacctggtat
    54601 ggcggcgaaa tgaagaaagg gatgttctcg atgatgaact acctgctgcc gctgaaaggt
    54661 atcgcttcta tgcactgctc cgccaacgtt gacgagaaag gcgatgttgc ggtgttcttc
    54721 ggcctttccg gcaccggtaa aaccaccctt tccaccgacc cgaaacgtcg cctgattggc
    54781 gatgacgaac acggctggga cgatgacggc gtgtttaact tcgaaggcgg ctgctacgca
    54841 aaaactatca agctgtcgaa agaagcggaa cctgaaatct acaacgctat ccgtcgcgat
    54901 gcgctgctgg aaaacgtcac cgtgcgtgaa gatggcacta tcgactttga tgatggttca
    54961 aaaaccgaga acacccgcgt ttcttatccg atctatcaca tcgataacat cgttaagccg
    55021 gtttcaaaag cgggtcacgc aacgaaagtt atcttcctga ccgcagacgc ctttggtgtg
    55081 ctgccgccgg tttctcgtct gactgccgat caaacccagt accacttcct ctctggcttc
    55141 accgccaaac tggccggtac tgagcgtggc atcaccgaac cgacgccaac cttctccgct
    55201 tgcttcggcg cggcattcct gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa
    55261 cgtatgcagg cggcgggcgc gcaggcttat ctggttaaca ctggctggaa cggcactggc
    55321 aaacgtatct cgattaaaga tacccgcgcc attatcgacg ctatcctcaa cggttcgctg
    55381 gacaacgcag aaactttcac tctgccgatg tttaacctgg cgatcccgac cgagctgccg
    55441 ggtgttgaca ctaagatcct cgatccgcgt aacacctacg cttctccgga gcagtggcag
    55501 gaaaaagccg aaacccttgc gaaactgttt atcgacaact tcgataaata caccgacacc
    55561 cctgcgggtg ccgcgctggt agcggctggt ccgaaactgt aatgatttga agctggagaa
    55621 tatctatcca gtatcttata gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta
    55681 cccttctttt gtcgtgccct gcgcccgcgt taccggcaca ggcagccagg cgcgaatgga
    55741 aagcccgccc cgctcgctgg tgccaagctc cagcatcccg ttatggttat ccacgatacg
    55801 ctgcacaatc gccagcccta atcccgtgcc gctaatggtg cgtgcactgt cgccgcgaac
    55861 aaacggctgg aaaaggtgct tacgttgttc cggcgcaatt cccggaccgt catcttccac
    55921 ctggaaccag gcgcgattcg gctccgtccc gctgctgact ttgatccagc cattgccgta
    55981 acgggcggcg ttgaccacca tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt
    56041 cacttcaatg ctgccggggt aaagcgcggt ttcaatttcc cgctcatagc cactttcggc
    56101 agcaataacc tcaccgagta ctgcgttgag atccgccatt tccatcggca tctcctgtcc
    56161 ggtgcgcagg tagtcgataa actgctcaat gatggcattg cactcctcga tatctttatt
    56221 gatcgattct gccaggtagc catcctgctc gctcatcatc tcggtcgcca ggcgaatacg
    56281 cgtcagcggt gtacgcaagt cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc
    56341 cagttgctta acaccagccg ccatatggtt aaaggcgcgg gtaacggaac gcacctccga
    56401 agcgccatat tcacgcagcg gcggcggaat aatcccttta ccgacctgca aagcagcatg
    56461 ttcgagatcg accaacggtc ggttctggat acgaataaac agccacgccc cgcctatcgc
    56521 caatagcata atcgccaacg tatagcggaa cagcggagag aaatcgccct gatgaatttc
    56581 ggtcagcggc acgcgtaccc agatattggg cgacagccag gttttcagcc agacgacagg
    56641 cgaacttttg ttgacctcaa cgcgcacttc cgtcgggccg cccagttgct gcgccatctg
    56701 atggcttaag aattcatagt gttgcgccca acgcagacct gcctcttcgg cagcctcgtt
    56761 ggagtagaga gagatcccca gctcacggta gatctcccga cggaaagcgg gaggcacaac
    56821 caactgcgtg ccgtcctcca gttgcagttt gtcggtcatc aacatacgca cttcgtacgc
    56881 gaggacttta ttaaactgct ggaggctcgg caaaatcgcg aagttcagca ccaccagata
    56941 agtcgtcacc aggctggcga acagcaaggt gacgatgagc aataacgtac gggcaaatga
    57001 acttcgtggc gagaagcgca atcgcctcat gctttagagc cgtccggtac aaagacgtag
    57061 cccaggcccc aaacggtctg aatgtaacgc ggatgcgctg gatcttcttc caccatgcgg
    57121 cgcagacgcg aaatctgcac gtcgatggag cgttccattg cggaatattc acgaccacgg
    57181 gcaaggttca tcagcttatc gcgggagagc ggctcacgcg gatggctgac cagtgccttc
    57241 agtaccgcaa actcaccgct ggtgagcggc atcggctcgt cttcgcggaa catttcgcgc
    57301 gtaccgaggt taagtttgaa cttaccgaaa gcaattaccg cctcttcctg tgatggtgcg
    57361 cctggtagct cgttcgcctg acgacgaagc accgcacgga tacgggccag cagttcacgc
    57421 gggttaaacg gttttggaat gtaatcgtca gcgccaatct ccaggccaac gatacggtcc
    57481 acttcttccc ctttcgccgt caccataatg atcggcatcg ggttgctctg gctacgaaga
    57541 cgtcggcaaa tcgacaagcc atcttcacca ggtaacatta aatccagtac cataagatgg
    57601 aaagattcac gagtcagcag gcgatccatc tgttctgcat tagcgacgct tcgaacctgg
    57661 aagccttgtt cggtgagata acgctccagc agcgcgcgca ggcgcatgtc gtcatcgacc
    57721 accagaatct tgtagttctc ttgcattgtt tgtactccca aaggttcgca acaatttgta
    57781 agcgtgtatt cttaaaaaag ctcacgttcg tcaccagcta aatctggtat gaatttcagc
    57841 ctaaattgtt acaaagcata ttaaacagca gcttaagtat acaatttatt cggcgaaaca
    57901 ttattgattc tgttgatatg atcacgttat acccaatgtg cgcattatca aacagacaaa
    57961 gggaatcaac gagatgaaaa cgcccctggt tacccgggaa gggtatgaaa aactcaaaca
    58021 agagcttaat tatctctggc gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc
    58081 cgcaagtctg ggcgaccgca gcgaaaatgc tgactatcag tataataaaa agcgcctgcg
    58141 tgaaatcgac cgtcgcgtgc gctatctcac taaatgcatg gaaaatctca aaatcgtcga
    58201 ttactccccg cagcaggaag gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga
    58261 cgatggcgtg actcaccgtt tccgtattgt cggctacgat gaaatttttg gccgtaaaga
    58321 ttacatctct atcgattccc cgatggcccg cgcattgctg aaaaaagaag tcggcgatct
    58381 ggcggtggtg aatacccctg ccggggaagc gagctggtat gttaatgcta tcgagtacgt
    58441 gaaaccgtaa ggaagagtct taacctcctg ccgatggctg gcatttttgc cagccagtcc
    58501 gtataactat cccctgattt ttgatccgaa aagatgaact caaaccatga tgaatgattc
    58561 gttctgccgc attattgcgg gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt
    58621 tcgcctgctt gacgaaggga ataccgtgcc gtttatcgca cgttatcgta aggaaatcac
    58681 cggcggtctg gatgacacgc agctgcgtaa tctggaaacg cgtctgagct atctgcgcga
    58741 gctggaagag agacgtcagg cgatcctcaa gtccatttcc gagcaaggca aactcaccga
    58801 tgatctggcg aatgccatca acgccaccct aagcaaaacc gaactcgaag acctctacct
    58861 gccctacaaa cctaaacgcc gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc
    58921 gttggctgac ctgctgtgga gcgatccgtc acacacgcca gaagtcgccg ctgcacaata
    58981 tgttgatgcc gataaaggcg tggcagatac caaagccgcg ctggatggcg cgcgctatat
    59041 cctgatggaa cggtttgccg aagatgccgc gctgctggcg aaagtgcgtg attatctgtg
    59101 gaagaacgcg catttggttt ctacggtggt gagcggtaaa gaaggggaag gggcgaaatt
    59161 ccgcgactat ttcgatcatc acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc
    59221 gatgttccgt gggcgtaacg aaggcgtact ccagctttcg ctgaatgccg atccgcagtt
    59281 cgatgagccg ccaaaagaga gctattgcga gcaaatcatc atggatcatc tcggcctgcg
    59341 cctgaacaat gctccggcgg atagctggcg caaaggcgtg gtgagctgga cctggcgcat
    59401 caaggtgctg atgcatctgg aaaccgaact gatgggcacc gtgcgcgaac gcgcggaaga
    59461 tgaagcaatc aacgtctttg cccgtaacct gcacgatctg ctgatggcgg cccctgccgg
    59521 actgcgtgca acgatgggcc tcgatccggg tctgcgtact ggggtaaaag tggcggtggt
    59581 cgatgcaact ggcaaactgg tagcgaccga taccatttac ccgcacaccg gacaagccgc
    59641 aaaagcagcg atgaccgttg ctgccttgtg tgaaaaacat aacgttgaac tggtggcgat
    59701 cggcaacggt acagcttccc gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt
    59761 cccgaaagtg accgcacaga aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc
    59821 ttccgagctg gcagcgcagg agttcccgga tctcgacgtt tcgctgcgtg gcgcggtgtc
    59881 tatcgcccgc cgtttgcagg atccactggc ggagctggtg aaaatcgatc cgaaatctat
    59941 cggcgtaggt cagtatcagc atgacgtcag ccagacgcaa ctggcccgca aactggatgc
    60001 agtagtagaa gactgcgtaa acgccgttgg cgtcgatctc aacaccgctt ctgttccgct
    60061 gttaacccgc gtggcgggcc tgacgcgcat gatggcgcaa aacatcgttg cctggcgcga
    60121 tgagaacggt cagttccaga accgtcagca actgttaaaa gtgagccgtc tggggccgaa
    60181 agccttcgag cagtgcgcgg gcttcttgcg cattaaccac ggtgataacc cgctggacgc
    60241 gtctaccgtt cacccggaag cctatccggt ggtggaacgc attctggcag caacacagca
    60301 ggcactgaaa gatctgatgg gtaacagcag cgagctgcgt aacctgaaag cgtctgactt
    60361 tactgatgaa aaattcggtg tgccaacagt aactgacatc atcaaagagc tggaaaaacc
    60421 gggtcgcgat ccgcgtccgg aatttaaaac cgctcagttt gccgatggcg tcgagacaat
    60481 gaacgacctg caaccgggta tgatcctcga aggtgcggtg accaacgtca ccaactttgg
    60541 cgcgtttgtc gatattggcg tgcatcagga cggcctggtt cacatctctt cattgtcgaa
    60601 caagtttgtg gaagatccgc ataccgtggt gaaagcgggc gacattgtga aggtgaaagt
    60661 gctggaagtg gatcttcagc gtaaacgtat cgccctgact atgcgtctgg atgagcagcc
    60721 tggcgaaacc aacgcccgtc gcggcggcgg taatgaacgc ccgcaaaaca accgcccggc
    60781 agccaaacca cgcggtcgta aagcgcagcc tgccggtaat agcgcgatga tggatgcgct
    60841 ggcggcggca atgggcaaaa aacgttaaac gcccgtactg gcccctaaac ctgcgcgata
    60901 aggcgttcac gccgcatctg gcaacgaacg ccttgcctga cataaaagtg ccggagaata
    60961 tctccggcat ttttattcca cagccaaact cacaatatat tccggcaaca tttatcactt
    61021 cattaacaac tgaaacctta attaaacatt agccagtccg ggtaattcac tattcgaatt
    61081 atattttcgc tgcgatataa ccttgagcca catcaacatt gagtcagatt attattcaaa
    61141 ccaacattcg cacacatttt aagtattgct gatagaaacc attctcatta tcattgtact
    61201 gttgattatt taatctctcc ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg
    61261 ccccatgagg tagttatcca gttaatgaga aacaagtagg cacctatgca atacactcca
    61321 gatactgcgt ggaaaatcac tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa
    61381 ctgctttctc ttggcatgtt acctggctcc tcttttaatg tggtgcgcgt cgctccactc
    61441 ggcgacccca ttcatatcga aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg
    61501 gccttattag aagtggaagc ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt
    61561 aaccataggc ttaattggta atccaaattc tggcaagaca accttattta accagctcac
    61621 tggcgcacgt cagcgtgtag gtaactgggc tggcgttacc gtcgaacgta aagaagggca
    61681 attctccacc accgatcatc aggtcacgct ggtggacctg cccggcacct attctctgac
    61741 caccatctca tcgcagacct cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg
    61801 cgacgccgac ctactgatta acgtggtgga tgcgtctaac cttgagcgta acctgtacct
    61861 gacgctacaa ctgctggaac tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat
    61921 tgccgagaag caaaatattc gtattgaaat tgatgctctg tcggcgcgtc ttggctgccc
    61981 ggtgatcccg ctggtttcaa cccgtggtcg cggtattgaa gcgctcaagc tggcgattga
    62041 tcgctataaa gctaacgaga atgtggaact ggtgcattac gcacagccgc tgctcaacga
    62101 agcagattca ctggcaaaag tgatgccttc cgacatcccg ctgaaacaac gtcgctggct
    62161 gggcctgcaa atgctggaag gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca
    62221 gcatcttgat gccgccctcg cccgtctgcg taatgagatg gacgatccgg cgctgcacat
    62281 tgccgatgcg cgttaccagt gcattgctgc catctgtgat gtggtaagca acaccctgac
    62341 ggcagaaccc agccgtttca ccactgcggt agataaaatc gtgctcaacc gtttcctcgg
    62401 tctgccgatt ttcctctttg tgatgtacct gatgttcctg ctggctatca acatcggcgg
    62461 ggcgttacag ccgctgtttg acgtcggctc cgtggcgcta tttgtgcatg gtattcaatg
    62521 gattggctac acgctccact tcccggactg gctgactatc ttcctcgccc agggcctggg
    62581 tggcggtatt aacaccgtgc tgccactggt gccgcagatc ggcatgatgt acctgttcct
    62641 ctccttcctt gaggactccg gctatatggc gcgtgcagcg tttgtgatgg accgcctgat
    62701 gcaggcgctg ggcttgccgg ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa
    62761 cgtaccgtcg gtaatgggcg cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat
    62821 catgatggca ccgtttatgt cctgcggcgc gcgtctggct atcttcgcag tattcgcggc
    62881 tgccttcttc gggcagaacg gtgcgctggc ggtcttctcg ctgtatatgc tcggtatcgt
    62941 gatggcggtg ctgactggcc tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc
    63001 gtttgtcatg gagctgccgg tctaccatgt accacacgtt aaaagcctga ttatccagac
    63061 ctggcagcgc ctgaaaggct tcgttctgcg tgctggtaaa gtgattatca tcgtcagcat
    63121 tttcctgagc gctttcaaca gcttctcgtt gagcgggaaa atcgtcgata acatcaacga
    63181 ctcggcgctg gcttccgtca gccgggtgat caccccggtc ttcaagccga ttggcgtgca
    63241 tgaagataac tggcaggcaa cagttggcct gtttacgggt gcaatggcga aagaagtggt
    63301 ggtgggtacg ctcaacaccc tctacaccgc agaaaatatt caggacgaag agttcaatcc
    63361 ggcggaattt aacctcggtg aagagttgtt cagcgcggta gatgaaacct ggcagagcct
    63421 gaaagacacc ttcagcctta gcgtactgat gaaccccatt gaagctagca aaggcgacgg
    63481 cgaaatgggt accggggcga tgggtgtgat ggatcagaaa ttcggtagcg cagctgccgc
    63541 atacagctac ctgattttcg tcctgctgta tgtaccgtgc atctcggtga tgggagccat
    63601 cgcccgtgaa tcaagccgtg gctggatggg cttctccatc ctgtgggggc tgaatatcgc
    63661 ttactcactg gcaacattgt tctatcaggt cgccagctac acccaacatc caacttacag
    63721 cctggtgtgc attctggcgg ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg
    63781 cgcgcgtagc cgggtggata tcgaactgct ggcaacccgc aagtcggtaa gcagttgctg
    63841 cgcagccagc accaccggtg attgccatta atggcttcac ttattcaggt gcgtgatttg
    63901 ctggcgttac ggggccgtat ggaagcggcc cagataagcc agacattgaa cactccacag
    63961 ccaatgatta acgccatgct gcaacaactg gaaagtatgg gcaaagccgt gcggattcag
    64021 gaagaacctg acggctgcct ctccggcagt tgtaaaagct gcccggaagg aaaagcctgt
    64081 ctgcgcgagt ggtgggcgct gcgttaacca tactccatcg cctgttttga aaagcccggt
    64141 atgcgtttgc atccgggctt ttttgcgtgc ggctttccat aaaaatgcaa ttcttgcagc
    64201 tcgacgtaag ttcctctgaa agcgtctcgc agggatgaaa actggctaat gcacaggtgt
    64261 ggagtggcgc gtagagtcgc ggcattcaaa caacaggtga aggaacgcca tgagcaaaaa
    64321 gcagagttcc accccacacg atgcgctgtt caaactcttt ttacgccaac cggacacggc
    64381 acgcgatttt cttgcgtttc atttaccggc acccattcac gcgctctgtg atatgaaaac
    64441 cctcaagctg gagtcgagca gctttattga tgacgatctg cgtgaaagct attccgatgt
    64501 gctgtggtcg gtgaaaacag aacaaggacc aggatacatc tattgtctga ttgaacatca
    64561 aagcacctca aacaaactga tcgcatttcg catgatgcgt tacgctattg ccgcaatgca
    64621 aaatcacctt gatgccggat acaaaacgtt gccgatggtg gtgccattgt tgttttacca
    64681 cggtattgaa agcccctatc cctattcgct gtgttggctg gattgtttcg ccgatcccaa
    64741 actggcaagg cagctttatg cctccgcatt tccgctgatt gatgtcaccg tcatgcctga
    64801 tgatgaaatc atgcagcacc gacgtatggc gctgctggag ttaattcaaa aacatattcg
    64861 tcaacgcgat ctgatggggc tggtagaaca aatggcctgc ttattaagta gtggatacgc
    64921 taatgacaga caaatcaaag ggctgtttaa ttacatcttg caaaccggcg atgcggtacg
    64981 ttttaacgat tttatcgacg gcgttgccga acgttcaccg aaacacaagg agagtttaat
    65041 gactattgcg gaaagattgc ggcaggaggg ggaacaatcc aaagccttgc atatagccaa
    65101 aataatgctt gaatccggag tccctcttgc agacatcatg cgctttaccg ggctgtcaga
    65161 agaagagttg gctgcggcga gtcagtaaag ttctgtctcg ccattcaaaa gtgcctacac
    65221 cctctgcttc aacgccacca gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc
    65281 atgggccgct ttggcgaaga tatacgactc gctgtgcggc cagagtttat ccagcatcgg
    65341 caccactttg cgcggcacca gaccgtcgag ataaccatac agtcgcaaaa acggcatggg
    65401 tacgttttgc agcggctggc ggagatcgac cgttttcagg atttccagcc caccattgag
    65461 cacgtcaacc tccggcatcg gtaacgccag aacggttttc ttcaacgccc gcgcatcctg
    65521 acgcgccgtt tcagtaccca tggtttgtaa cgcaaggaac cgttccaccg tacgctgaaa
    65581 atcatcactg agttgctgct gaaatcccgc cagcacgtcc ggttttatcc ccggccactc
    65641 gtcacgagca ctaaaacaag gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg
    65701 atgggttaac gcaatctggc ttgccaccag cccgcccaga ctccagccta accaaatggc
    65761 tttatcaggt gcctgtcgca gcacggcttc ggccatatca gcaagtgaca tcgcaccaaa
    65821 tccccggcta cgcccgaagc cgggcaggtc aacaaggtgc agcgtaaaat gcgagctcag
    65881 ttcctcgtca atgcaacgcc acacttcggc attcagtccc catccgtgca gcagcacaag
    65941 atgaacattc ccctgacctt tggtctgcca ccagatgtta ttcatccgct attgttctct
    66001 tttgacttac aaggatgaac atatgctaac agtaccggga ttatgctggc tatgccgaat
    66061 gccactggca ttaggtcatt gggggatttg ttcggtctgc tcacgcgccg cccgtacaga
    66121 taaaacgtta tgcccacaat gtggattacc cgccacacac tcccatcttc cctgcggtcg
    66181 ctgcctgcaa aaaccgccgc cctggcaaag actggtcacg gttgccgact atgcgccgcc
    66241 gttaagtctg ctcatccacc agcttaagtt ttcccggcgc agcgaaatcg ccagcgccct
    66301 gtcacgtctg ttactattgg aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc
    66361 ggatcgtatc atcagcgttc cgttatggca gcggcgtcac tggcgtcggg gatttaatca
    66421 gagcgatttg ctgtgtcagc cgttatcacg ctggttgcac tgccaatggg atagcgaagc
    66481 cgtcacacgt acacgggcca ctgcgaccca gcattttctt agcgcccggc tacgcaggcg
    66541 caacctgaaa aatgcctttc gccttgaatt gcctgtgcaa ggtcgccata tggtgattgt
    66601 ggatgatgtc gttaccaccg gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa
    66661 tggtgcggcg actgtccagg tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatgg
    66721 gcgtattata accaactaaa atagtcaact attaggccat tactatgatc cgtatttccg
    66781 atgctgcaca agcgcacttt gccaaactgc tggcaaatca ggaagaaggg acacaaatcc
    66841 gcgtatttgt gattaaccct ggcacgccta acgctgaatg tggtgtttct tattgtccgc
    66901 cggatgccgt ggaagccacc gacaccgccc tgaaatttga cctgctgacc gcgtatgttg
    66961 atgagttaag cgcaccatac ctggaagatg cagagatcga ttttgttacc gaccagttgg
    67021 gttcccagtt aacgctgaaa gccccgaacg ccaaaatgcg taaagtggca gacgatgcac
    67081 cgctgatgga gcgcgtggag tatatgctgc agtcgcagat caacccgcaa cttgctggtc
    67141 acggtggtcg cgtttcgctg atggaaatca ccgaagacgg ttacgccatt ctgcaatttg
    67201 gcggcggctg taacggttgt tccatggtgg atgtgacgct gaaagaaggg atcgagaagc
    67261 agctgctaaa cgaattcccg gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg
    67321 gcgaacactc ctactactaa gttatcccct cttttgtaga ttgcccgatg cgacgctaaa
    67381 gcgtcatttc gggcctacgt tgaacatgtg ccagacgttg gcgcagcatt gctttggcaa
    67441 atccctccct tcgtttcccc gcgataatat gaccaacctc tcataattta aatttacccc
    67501 gctctggtga ttctcaaacg ccagatgtta cccgtatcat tcacgtgggt accaaacata
    67561 ctcctgacat ctgactacaa taattagttt tagtgggtat cagtcgtggt gccgcaatat
    67621 ctctgttccc gattgggata attagagctt gtcgtcagaa aattgacgtt acccataaca
    67681 aatgaaaggc caggtaaatc atgccattag tcattgttgc tatcggtgta atcttgttat
    67741 tactcctgat gatccgcttc aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg
    67801 ctgttggatt aatgcaagga atgccgctgg ataaagttat tggctccatc aaagccggtg
    67861 tcggcgggac gctcggtagc cttgccctga tcatgggttt tggcgcaatg ctgggcaaaa
    67921 tgctggcaga ctgcggtggc gcacaacgta tcgccaccac gctgattgcc aaatttggta
    67981 aaaaacacat ccagtgggcg gtggtactga ccggttttac cgttggtttt gccctgttct
    68041 atgaagtggg ctttgtgctg atgctgccgc tggtgtttac catcgcggct tctgcgaata
    68101 ttccactgct gtatgttggt gtaccaatgg cggctgcact gtctgtgacc cacggcttcc
    68161 tgccaccgca tccgggcccg actgcgattg ccaccatttt caatgccgac atgggtaaaa
    68221 ccctgctgta cggtactatt ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg
    68281 ctcgcgtgtt gaaaggtatc gataagccaa tcccggaagg tctctacagc gcgaaaacct
    68341 tcagcgaaga agagatgccg agctttggcg tcagcgtctg gacctctctg gtgccggtag
    68401 tactgatggc gatgcgtgcg attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc
    68461 cggtagcgga gttcctcggt gacccggtaa tggctacgct gattgccgtg ctgattgcga
    68521 tgttcacctt tggtctaaac cgtggtcgtt caatggatca gattaacgac acgctggttt
    68581 cttccatcaa aatcattgcg atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc
    68641 aggtgctggt agacagcggc gtggacaaat acattgcttc catgatgcac gaaaccaata
    68701 tttctccgct gctgatggcc tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg
    68761 caaccgttgc ggcaatcact gcgggtggta tcgcggcacc gctgattgca acgacgggtg
    68821 ttagcccgga gctgatggtt attgcggtgg gttccggtag tgtgattttc tctcatgtga
    68881 acgatccggg cttctggctg ttcaaagagt actttaacct gactatcggc gagaccatca
    68941 aatcctggtc gatgctggaa accattatct cggtgtgcgg tctggtaggc tgtctgctgc
    69001 tgaatatggt gatttgataa cactgactgc cggatgcatc gcatccggca tcaaccgcac
    69061 tctacttctt cttcgctgca gctctgcgcc gtctgtccaa atccttcagc aacttgttca
    69121 cgccatcatc ggcaaacatc gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat
    69181 actggtaact ggtgccagga atattcaccg gttcggccat atccagccag tcttccggct
    69241 gtagtcctaa cagagcactg ttactgtcgg caatgtagcg ctgcaaacca cggttcagcg
    69301 tcggcgtcat cgacatcaac gatgccttat gcccggcacg ttttggcaga caaccatatt
    69361 tatgcagtgc atccagcagc ccttgcttcg ccagttcgcg atcctgatac agaccgcgca
    69421 gtaccacttc atccggatac agccccaggg ttttgcccag cgttagatcc ccgctctccc
    69481 agtaaccacg cagcgttggc aggtcatgtg tcgccgcaac cgccatcgac tgctccggat
    69541 acgctttcgg tgcacggaac gtcttctcgt ggtcgttttc gaaatagagc actttgtagg
    69601 agtacacacc gctgctgcgc agcttaccga caatctctac cggtaccgta ccgagatctt
    69661 caccaatcac catacagcga tgacgtttac tttcgagtgc cagaatcgag agcagatcat
    69721 ccaccggata gtgtacatac gcgccctgat ctgccgtctc gccatacggt atccaccaca
    69781 aacgcagcat cgacatcaca tggtcaattc gtaatgcgcc gcagttttgc atattggcac
    69841 gcaacagctc gataaacggt tcataagcac gcgcggtgat gatatgcggg tccattggcg
    69901 gcaatcccca gttctgcccc aacgggccga ggatatccgg cggcgcgcca accgacgctt
    69961 tcaggcaata cagttcacgg tcgcaccaag tttccgcccc accttccgct acgccaaccg
    70021 ccagatcacg atacaagcca atcggcattt catagccctg gcttatctcc cagcaggcgg
    70081 caaactggct gtaagccagc cactgcagcc agagataaaa atcaacgtca tcgcgatgtt
    70141 cttcgcagaa ctgacgcact tctggtgaat ccacgttctg atacatctct ggccatgcag
    70201 gccagcccca gcgcatttcg tcctctttca cttgctgggc atgtagcgca tcaaaggctg
    70261 cctgccagaa caggctgtcg ccttgatctg caacaaactg gcgaaacgcg gccatctgct
    70321 catcatcacg ttgcgcgaaa cctttccacg ccattcgtaa tgctgtcatt tttagggcgg
    70381 taaccgtgga gtaatcgacc cagtcggcat cccgcgcctg ttgcagcgtc tgttgcgtgg
    70441 tcggcaactg ccaccaggcc tgagcctctt cgctaagatg gaaatcttcg acggcgttaa
    70501 cgtcgatata aatcacattc agccagcggc gagaagacgg gctgtatggg ctggcgctct
    70561 ccggatttgc cggatagagc gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt
    70621 ttgccacatc caccagcatc gctttgagat cgccaaaatc cccaataccc cagttttttt
    70681 ccgatcgcag cgtataaagc tggacgcagg caccccacag cttttgttta ttcagcaacg
    70741 cctgcggttc gtaacagcgt ttcggggcaa caatcaccct gcaatgcgcg cgctggtctt
    70801 cctgggtaag cgtcagcgtg tgataacctt ccggcagctt cgtcggtaga ttgaacgctt
    70861 tgcccccagt tacatggcct ttgtactgcg ttccttcttc ggtggtcagc agccagctat
    70921 attcgccgct gccctccacc accatcggca tttttttgcc gctggtataa accatgacat
    70981 tcgggactgg cgttaccgcc actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac
    71041 gccgtttggt ttcggcgcta atcgactgcg gtttaccgtg ggcattgatg taattggggc
    71101 taatccccgc cgccagcgcg gcattatcca gacgtttgct ttccatcgag cttccttagc
    71161 gttttgcctg ccagatacga gcctgataat cgcgaataga gcgatccgag ctaaacatac
    71221 cgcagcgggc ggtattgagg atcgccgcgc gagtccaggc ctcctggtcg cggtacagca
    71281 catccacctg cttttgtgcc tcaacatagg ctgcgaaatc cgccatcacc agatacggat
    71341 cgccgccctg tttgccgata ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc
    71401 tgtatttacc gctttccagc tctttcaata ctgcgtccag caccttatct ttcttccgcc
    71461 atttcaccgg gtcgtagcct ttggccagaa tagccttcac ttgttccacg gtatgaccaa
    71521 aaataaagat attttcttca ccgactttct cggcgatttc aacgttcgca ccatccagcg
    71581 taccgacagt aagcgcacca ttgagcgcca gtttcatatt gccggtaccg gaagcttctt
    71641 tacctgcagt cgaaatttgt tcggagatat ccgccgccgg gatcagtttt tccgccgccg
    71701 aaacgcaata atccggcagg aacaccacct tcaacttatc gccaaccagc ggatcgttgt
    71761 tgatcacgtc agccactttg ttgatcgcaa agataatatt cttcgccagg tagtagcccg
    71821 gtgccgcttt cgcgccgaag aggaagacgc gcggtacgcg atcagcctgc gggttttcac
    71881 gaatttcttt gtacaacgcc agaatatgca gcagattcag gtgctggcgt ttgtactcat
    71941 gcagacgttt gatctgaata tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa
    72001 ctttcacaaa ctccgccaga cggactttat tctcctgctt gatctcgcga tattgctgac
    72061 ggaatttcgc atcatcagcg aatttttcca gattgatcag ctgatcgaga tcgttagccc
    72121 actctttttt cagtgattta tccaacagag ccgccagtgc cgggttgcac tgtttgatcc
    72181 agcgacgtgg ggtaataccg ttggtgacgt tatggaattt gttcggccat agctggtgat
    72241 attccgggaa cagatctttc accaccagat ccgagtgcag cgccgcaaca ccgttcaccg
    72301 cgaaaccgcc aaccacacac aggttcgcca tatgcacttg tttgtcgtgc accaccgcca
    72361 gtttggccca cactttttca tcgcccggcc aggttttctc caccagcgtt ttaaagcgag
    72421 tattaatttc gttaataatc tgcatgtggc gcggcagtaa gcctttcacc agtttcacat
    72481 cccagcgttc cagcgcttct ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg
    72541 caatggccca ggcgtcatcc cagctcatct ggtgctcatc gatcagcacg cgcagcagtt
    72601 ctggaatcgc gatagttggg tgggtatcgt tcagctgaat aacttcgtaa tccgccagtt
    72661 cgtgcagttt acgccccgcc agatgatggc gacgcaaaat atccgctacc gaacaggcac
    72721 actggaagta ttgctgcatc aagcgcagct ttttaccggc agtatggttg tcgtttggat
    72781 agagaacttt ggtcagtttt tccgcattga tgccctgctg ttcggcacgc aggaaatcac
    72841 cgtcgttaaa tttagtcaga tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg
    72901 gctgcgccac gccattacga tagccgacaa cggggagatc ccacgcttga ccggtaatgg
    72961 taaactccgg ctcccagcgt ccgtctttcg tcactttacc gccaatccct acctgcacat
    73021 ccagtgcttc gttgtggcgg aaccacgggt agttactgcg atgccagtca tccggcgctt
    73081 caacctgttt gccatccaca aaagactggc ggaacaaacc atattgatag ttcagaccgt
    73141 aacccgtcgc agactgaccg acagttgcca ttgaatcgag gaagcacgcc gccagacgtc
    73201 ccagaccacc gttacccagc gccgggtcga tctcttcttc cagcaggtca gtcagattga
    73261 tgtcataagc cttcaacgaa tcctgtacat cctgatacca gccgagattc aacaggttgt
    73321 tgcccgtcag acgaccaatc aaaaactcca ttgagatgta gttaacatgt cgctgattcg
    73381 ccaccggctt ggcgaatggc tgagcacgca gcatttcggc cagtgcttca ctcactgcca
    73441 gccaccactg gcgaggagtc atttcagccg cagaatttaa gccataacgc tgccactgac
    73501 gtgaaagcgc ttcctgaaat tgcttatcgt taaaaatagg ttgtgacata ggagttccac
    73561 ttttcttaga ttttcaacac aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc
    73621 tcatcctgcg ggggattagg cagggaggag ttgcggggat gagcaaggaa atgtgatctc
    73681 aaccacttaa agctagtgca aaccacagga ttagcagcaa atcaatgcca tacagcgcag
    73741 aaaatctgta tctaagtgca aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac
    73801 tctataaatt aagtaaagga gtgaaacagt ttcacaagta aaat
//
LOCUS       DAPGRN010000009        72386 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPGRN010000009 DAPGRN010000000
VERSION     DAPGRN010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.009"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..84)
                     /locus_tag="RHW33_000663"
                     /pseudo
     CDS             complement(1..84)
                     /locus_tag="RHW33_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="enhances serine sensitivity caused by inhibition of
                     homoserine dehydrogenase I; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
     gene            complement(680..1456)
                     /gene="sseB"
                     /locus_tag="RHW33_000664"
     CDS             complement(680..1456)
                     /gene="sseB"
                     /locus_tag="RHW33_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV1644209.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLGSTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1598..2881)
                     /gene="pepB"
                     /locus_tag="RHW33_000665"
     CDS             complement(1598..2881)
                     /gene="pepB"
                     /locus_tag="RHW33_000665"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV1644210.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3126..3326)
                     /gene="iscX"
                     /locus_tag="RHW33_000666"
     CDS             complement(3126..3326)
                     /gene="iscX"
                     /locus_tag="RHW33_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV1644211.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3338..3673)
                     /gene="fdx"
                     /locus_tag="RHW33_000667"
     CDS             complement(3338..3673)
                     /gene="fdx"
                     /locus_tag="RHW33_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV1644212.1"
                     /translation="MPKIVILPHQELCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3675..5525)
                     /gene="hscA"
                     /locus_tag="RHW33_000668"
     CDS             complement(3675..5525)
                     /gene="hscA"
                     /locus_tag="RHW33_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV1644213.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFEASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGISDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDIDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5542..6057)
                     /gene="hscB"
                     /locus_tag="RHW33_000669"
     CDS             complement(5542..6057)
                     /gene="hscB"
                     /locus_tag="RHW33_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV1644214.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6153..6476)
                     /gene="iscA"
                     /locus_tag="RHW33_000670"
     CDS             complement(6153..6476)
                     /gene="iscA"
                     /locus_tag="RHW33_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV1644215.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPMPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6493..6879)
                     /gene="iscU"
                     /locus_tag="RHW33_000671"
     CDS             complement(6493..6879)
                     /gene="iscU"
                     /locus_tag="RHW33_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV1644216.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6907..8121)
                     /gene="iscS"
                     /locus_tag="RHW33_000672"
     CDS             complement(6907..8121)
                     /gene="iscS"
                     /locus_tag="RHW33_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV1644217.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8237..8725)
                     /gene="iscR"
                     /locus_tag="RHW33_000673"
     CDS             complement(8237..8725)
                     /gene="iscR"
                     /locus_tag="RHW33_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV1644218.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8995..9735)
                     /gene="trmJ"
                     /locus_tag="RHW33_000674"
     CDS             complement(8995..9735)
                     /gene="trmJ"
                     /locus_tag="RHW33_000674"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV1644219.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9854..10657
                     /gene="suhB"
                     /locus_tag="RHW33_000675"
     CDS             9854..10657
                     /gene="suhB"
                     /locus_tag="RHW33_000675"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV1644220.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            10802..11656
                     /locus_tag="RHW33_000676"
     CDS             10802..11656
                     /locus_tag="RHW33_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1644221.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            11847..13127
                     /gene="csiE"
                     /locus_tag="RHW33_000677"
     CDS             11847..13127
                     /gene="csiE"
                     /locus_tag="RHW33_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV1644222.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13119..14258)
                     /locus_tag="RHW33_000678"
     CDS             complement(13119..14258)
                     /locus_tag="RHW33_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV1644223.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14418..15308)
                     /gene="hcaR"
                     /locus_tag="RHW33_000679"
     CDS             complement(14418..15308)
                     /gene="hcaR"
                     /locus_tag="RHW33_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV1644224.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            15444..16805
                     /locus_tag="RHW33_000680"
     CDS             15444..16805
                     /locus_tag="RHW33_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV1644225.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYTEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16802..17320
                     /locus_tag="RHW33_000681"
     CDS             16802..17320
                     /locus_tag="RHW33_000681"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV1644226.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17320..17640
                     /locus_tag="RHW33_000682"
     CDS             17320..17640
                     /locus_tag="RHW33_000682"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV1644227.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            17637..18449
                     /gene="hcaB"
                     /locus_tag="RHW33_000683"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             17637..18449
                     /gene="hcaB"
                     /locus_tag="RHW33_000683"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV1644228.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIAGEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18459..19661
                     /locus_tag="RHW33_000684"
     CDS             18459..19661
                     /locus_tag="RHW33_000684"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV1644229.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDEQHLPYER
                     PPLSKSMLLEDSPQLQSVLPAHWWQENNVHLHSGVTIKTLGRDTRELVLANGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANDQLAREANLDTVNGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLSPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRSIRKWIQSGKTFDAKQLTD
                     ENIALKSL"
     gene            19758..20180
                     /locus_tag="RHW33_000685"
     CDS             19758..20180
                     /locus_tag="RHW33_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV1644230.1"
                     /translation="MNSLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20228..21100)
                     /locus_tag="RHW33_000686"
     CDS             complement(20228..21100)
                     /locus_tag="RHW33_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV1644231.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNHFVWQGREYQLQPNVEWDAHYLHGDGWLGQWQCASCSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIIETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21112..22206)
                     /locus_tag="RHW33_000687"
     CDS             complement(21112..22206)
                     /locus_tag="RHW33_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV1644232.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(22239..23237)
                     /locus_tag="RHW33_000688"
     CDS             complement(22239..23237)
                     /locus_tag="RHW33_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1644233.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23261..24772)
                     /locus_tag="RHW33_000689"
     CDS             complement(23261..24772)
                     /locus_tag="RHW33_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1644234.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAHKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVVLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRCGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(24795..25778)
                     /locus_tag="RHW33_000690"
     CDS             complement(24795..25778)
                     /locus_tag="RHW33_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV1644235.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(25875..29156)
                     /locus_tag="RHW33_000691"
     CDS             complement(25875..29156)
                     /locus_tag="RHW33_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV1644236.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGTQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHGLNKNPQCGQASLIRASAYERQGQYQLAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLAAAGLGEIAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            29274..30467
                     /locus_tag="RHW33_000692"
     CDS             29274..30467
                     /locus_tag="RHW33_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV1644237.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGEHWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVET
                     QLRQI"
     gene            complement(30531..31784)
                     /gene="glyA"
                     /locus_tag="RHW33_000693"
     CDS             complement(30531..31784)
                     /gene="glyA"
                     /locus_tag="RHW33_000693"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV1644238.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32112..33302
                     /gene="hmpA"
                     /locus_tag="RHW33_000694"
     CDS             32112..33302
                     /gene="hmpA"
                     /locus_tag="RHW33_000694"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV1644239.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVTEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33347..33685)
                     /gene="glnB"
                     /locus_tag="RHW33_000695"
     CDS             complement(33347..33685)
                     /gene="glnB"
                     /locus_tag="RHW33_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV1644240.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(33746..35080)
                     /gene="glrR"
                     /locus_tag="RHW33_000696"
     CDS             complement(33746..35080)
                     /gene="glrR"
                     /locus_tag="RHW33_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV1644241.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35070..35783)
                     /gene="qseG"
                     /locus_tag="RHW33_000697"
     CDS             complement(35070..35783)
                     /gene="qseG"
                     /locus_tag="RHW33_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV1644242.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDNGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(35948..37375)
                     /gene="qseE"
                     /locus_tag="RHW33_000698"
     CDS             complement(35948..37375)
                     /gene="qseE"
                     /locus_tag="RHW33_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV1644243.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELDNVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(37951..41838)
                     /gene="purL"
                     /locus_tag="RHW33_000699"
                     /gene_synonym="purI"
     CDS             complement(37951..41838)
                     /gene="purL"
                     /locus_tag="RHW33_000699"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV1644244.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADKEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DATHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42097..43653
                     /gene="mltF"
                     /locus_tag="RHW33_000700"
     CDS             42097..43653
                     /gene="mltF"
                     /locus_tag="RHW33_000700"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV1644245.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(43650..44186)
                     /gene="tadA"
                     /locus_tag="RHW33_000701"
     CDS             complement(43650..44186)
                     /gene="tadA"
                     /locus_tag="RHW33_000701"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV1644246.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44211..44846)
                     /gene="yfhb"
                     /locus_tag="RHW33_000702"
     CDS             complement(44211..44846)
                     /gene="yfhb"
                     /locus_tag="RHW33_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV1644247.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45055..45903
                     /locus_tag="RHW33_000703"
     CDS             45055..45903
                     /locus_tag="RHW33_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV1644248.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            45959..46219
                     /gene="yfhL"
                     /locus_tag="RHW33_000704"
     CDS             45959..46219
                     /gene="yfhL"
                     /locus_tag="RHW33_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV1644249.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
     gene            complement(46413..46493)
                     /gene="shoB"
                     /locus_tag="RHW33_000705"
     CDS             complement(46413..46493)
                     /gene="shoB"
                     /locus_tag="RHW33_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV1644250.1"
                     /translation="MTDCRYLIKRAIKIIIAVLQLILLFL"
     gene            complement(46914..47294)
                     /gene="acpS"
                     /locus_tag="RHW33_000706"
     CDS             complement(46914..47294)
                     /gene="acpS"
                     /locus_tag="RHW33_000706"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV1644251.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(47294..48025)
                     /gene="pdxJ"
                     /locus_tag="RHW33_000707"
     CDS             complement(47294..48025)
                     /gene="pdxJ"
                     /locus_tag="RHW33_000707"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV1644252.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(48037..48765)
                     /gene="recO"
                     /locus_tag="RHW33_000708"
     CDS             complement(48037..48765)
                     /gene="recO"
                     /locus_tag="RHW33_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV1644253.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGACSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGGTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(48777..49682)
                     /gene="era"
                     /locus_tag="RHW33_000709"
     CDS             complement(48777..49682)
                     /gene="era"
                     /locus_tag="RHW33_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV1644254.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(49679..50359)
                     /gene="rnc"
                     /locus_tag="RHW33_000710"
     CDS             complement(49679..50359)
                     /gene="rnc"
                     /locus_tag="RHW33_000710"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV1644255.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(50632..51606)
                     /gene="lepB"
                     /locus_tag="RHW33_000711"
     CDS             complement(50632..51606)
                     /gene="lepB"
                     /locus_tag="RHW33_000711"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV1644256.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(51622..53421)
                     /gene="lepA"
                     /locus_tag="RHW33_000712"
     CDS             complement(51622..53421)
                     /gene="lepA"
                     /locus_tag="RHW33_000712"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV1644257.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPILNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(53619..54098)
                     /gene="rseC"
                     /locus_tag="RHW33_000713"
     CDS             complement(53619..54098)
                     /gene="rseC"
                     /locus_tag="RHW33_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV1644258.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(54095..55051)
                     /gene="rseB"
                     /locus_tag="RHW33_000714"
     CDS             complement(54095..55051)
                     /gene="rseB"
                     /locus_tag="RHW33_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV1644259.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(55051..55701)
                     /gene="rseA"
                     /locus_tag="RHW33_000715"
     CDS             complement(55051..55701)
                     /gene="rseA"
                     /locus_tag="RHW33_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV1644260.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(55734..56309)
                     /gene="rpoE"
                     /locus_tag="RHW33_000716"
     CDS             complement(55734..56309)
                     /gene="rpoE"
                     /locus_tag="RHW33_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV1644261.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(56306..56461)
                     /gene="rseD"
                     /locus_tag="RHW33_000717"
     CDS             complement(56306..56461)
                     /gene="rseD"
                     /locus_tag="RHW33_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV1644262.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            56717..58339
                     /gene="nadB"
                     /locus_tag="RHW33_000718"
     CDS             56717..58339
                     /gene="nadB"
                     /locus_tag="RHW33_000718"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV1644263.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERN
                     NAVDLIISDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALDHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(58324..59061)
                     /gene="trmN"
                     /locus_tag="RHW33_000719"
     CDS             complement(58324..59061)
                     /gene="trmN"
                     /locus_tag="RHW33_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV1644264.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     IKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAASQAQENVAQSPWLERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECSTPQRKQARYTTTLDHPSLLACAAECIT
                     EEGFFCVVLPEQIGNSFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            59193..60527
                     /gene="srmB"
                     /locus_tag="RHW33_000720"
     CDS             59193..60527
                     /gene="srmB"
                     /locus_tag="RHW33_000720"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV1644265.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(60560..61441)
                     /locus_tag="RHW33_000721"
     CDS             complement(60560..61441)
                     /locus_tag="RHW33_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1644266.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPYIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEHSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEDCFLPG"
     gene            61544..62131
                     /gene="eamB"
                     /locus_tag="RHW33_000722"
     CDS             61544..62131
                     /gene="eamB"
                     /locus_tag="RHW33_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV1644267.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWIVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(62187..62570)
                     /gene="grcA"
                     /locus_tag="RHW33_000723"
     CDS             complement(62187..62570)
                     /gene="grcA"
                     /locus_tag="RHW33_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV1644268.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            62875..63564
                     /gene="ung"
                     /locus_tag="RHW33_000724"
     CDS             62875..63564
                     /gene="ung"
                     /locus_tag="RHW33_000724"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV1644269.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(63612..64649)
                     /locus_tag="RHW33_000725"
     CDS             complement(63612..64649)
                     /locus_tag="RHW33_000725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV1644270.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            64856..65275
                     /gene="trxC"
                     /locus_tag="RHW33_000726"
     CDS             64856..65275
                     /gene="trxC"
                     /locus_tag="RHW33_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV1644271.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            65344..66042
                     /gene="tapT"
                     /locus_tag="RHW33_000727"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             65344..66042
                     /gene="tapT"
                     /locus_tag="RHW33_000727"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV1644272.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAEAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQNLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            66074..68734
                     /gene="pat"
                     /locus_tag="RHW33_000728"
     CDS             66074..68734
                     /gene="pat"
                     /locus_tag="RHW33_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV1644273.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAKAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMTWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            68848..70203
                     /gene="pssA"
                     /locus_tag="RHW33_000729"
     CDS             68848..70203
                     /gene="pssA"
                     /locus_tag="RHW33_000729"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV1644274.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVQVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            70249..70572
                     /locus_tag="RHW33_000730"
     CDS             70249..70572
                     /locus_tag="RHW33_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV1644275.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(70569..71867)
                     /gene="kgtP"
                     /locus_tag="RHW33_000731"
     CDS             complement(70569..71867)
                     /gene="kgtP"
                     /locus_tag="RHW33_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV1644276.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(72190..72305)
                     /gene="rrf"
                     /locus_tag="RHW33_000732"
     rRNA            complement(72190..72305)
                     /gene="rrf"
                     /locus_tag="RHW33_000732"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        17294 a        19635 c        18202 g        17255 t
ORIGIN      
        1 gattatccga ttctgtttaa agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg
       61 cgcaatatgt tcgatattaa aatgacctgg cttattagtc cgaattcaga caaatataaa
      121 caaatcccgc tcaatattaa aaatactaac cggtaaaaga tattacttaa acatgtaaat
      181 tcactttctt taagcaacaa aaaaccgcca aaatcaggcg gttatttgtt gccggtttgg
      241 ttcgcggcct ctccagcaga ttgacttgtg ttgcatgagc aacgcaggtg cttcacagca
      301 gagcaatatt cgccagcaac tctctttttg tcaagcaaaa gagagtaatt attgtttatt
      361 tagcgtatta tcgacaccgg ccctttccgc cgtctcgcaa acgggcgctg gctttaggaa
      421 aggatgttcc atgaccgtaa atgcaggtgt ttcacagcgc ttgctatcgc ggcaatatcg
      481 ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat acggtgcttt
      541 tgtatcgtac ttattgtttc tggtgcgctg ttaaccgagg taaataataa cctgagtctc
      601 tccggcaaca atttactggt ggttaacaac cttcagaaca gcaggtaagc ccgaatgccg
      661 ccctttgggg cggcatattt tagattatcc ggttctgttt aaagtcacgc aaaaaaccac
      721 cccaacgacg ttcatagaat ggcgcaatat gttcggtaat aaagtggcta attccttttt
      781 cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta tcggtcgcta
      841 cacttcccgc cgcctgaata atttcttcga tatcaccatc ggcttcaatg ccaataagta
      901 aattaggctg tgcctcttcg ttctctttaa ttgaacatat aaaagcacgc ttcaccggct
      961 taatagtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc tctgcgactt
     1021 ccgataatat tagcgattca ccgccttcca gtacttcctg gctactcagc ggattccctt
     1081 cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtt ggcagtttgg
     1141 cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg ggcattacga
     1201 caaatgcctg ttcgtcttca accgcctgtt gaagtgcttc taacgaggta aaaaaaggaa
     1261 tgacgctggt gccgtcttct ttttcccaat gctgtaaatc aagcgcacta tcttcaacca
     1321 cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggatcccagt agagtacgga
     1381 aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct tcaagttcgt
     1441 tttttgtttc ggacataaga accacaattc attcaacgtt cggggcacaa gtgcctgatg
     1501 cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag aatttgtagg
     1561 ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac agattagcaa
     1621 tcgtgcgcac accaagtccc gtagcacccg cagaccactg ttcaaccggc gctttacggt
     1681 aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca aagtgcgaca
     1741 ggaagcccgc tgccgtgctc gcgcctgccg gatacgccgc gcttccggta ttgttcagtt
     1801 cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga cgccagaatg
     1861 gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg tcaaaactga
     1921 acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg gtcgccgcat
     1981 caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca agcaccaggc
     2041 gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag gtgatgatat
     2101 cgcccagctt aaacgcattg ccgctgatca ggttatccgc acagcagagg aacagcttca
     2161 cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc gttgccgcgc
     2221 cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg tagccgccgg
     2281 agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct ttatcgccag
     2341 ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt ccgacggtgt
     2401 gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga taagtcacac
     2461 gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc gatggtccca
     2521 attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc aggcggttat
     2581 ccagttcctg gcgctgggca tcgtccaaat ccggccactc cactttacgc gtgcctttcg
     2641 gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct tcaccgctta
     2701 actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg atcagcccca
     2761 gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg taagttgctt
     2821 tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc gcttctgtca
     2881 ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat ttttccgcta
     2941 aatgattcgc gttgcaggac aaaacgcatt gcgttttgaa cagcgcttgc gctggccccg
     3001 gaggggtgag gccaaaaggc cgaatcacgt ggcaagtgag tgaagcccca ggagcataga
     3061 taactatgtg actggggtga acgagcgcag ccaacgcatc tgcggcgtga agcatgacgc
     3121 ggaaattatt cggcttcgtc cagccagact aacaaaatcg cttcgaggat tttctcgttg
     3181 gatgcctgcg ggtcgtcgtc gaaatcttcc agatcgcaaa tccactgatg catatcggtg
     3241 aatcgaaccg ttttcggatc aagatcggga tacgcatcgt acagtgcttc gccaatttcg
     3301 cggctatcgg tccacttaag tcccatacta acctctgtta atgctcacgc gcatggttga
     3361 tcgtgtaacg cgggatttcg accactaaat cttcgtcggt gacgcgcgcc tggcagctta
     3421 aacggctttc cggctccagt ccccaggctt tgtccagcat gtcgtcttcc tgctctgagc
     3481 tttctggaag cgagtcaaat ccttcacgaa cgatgcagtg gcaggtggtg caagcacagg
     3541 atttttcaca ggcgtgttca atctcgatac cgttacgcag agctgcgtcg agaatggttt
     3601 caccgctatt agcttccaga acagcgccat cagggcagag ctcctgatga ggcaaaataa
     3661 caatctttgg catattaaac ctcgtccacg gaatggcctt tcagcgcacg acgaaccgac
     3721 tggtccatgc ggcgagcggc gaaatcctgg gtttgtttgt ctacgttttt aatcgcttgt
     3781 tcgatggcgt caatatcatc gccctgcgcc acttcactca ggtgagcggc agcatcgtca
     3841 atgacctgac gttctgcggc gcttaacagc gcggcatcag cagccagcgc gccgtgcaga
     3901 ctttccagca cacgcgccgc ttctactttt tgttctgcca gcattcgggc ttttacgtcc
     3961 tgctcggcat agctcattga gtctttgatc atcgaagcga tttcgctatc ggtcagaccg
     4021 taagacggtt tgacctgaat agacgcctca acgccggtgg atttctccat cgccgtcacg
     4081 ctcaaaagac cgtcggcatc gacctggaac gtcacgcgaa tatgcgcacc gccagccggt
     4141 agcgccggaa taccacgcag cgcaaaacgc gccagtgagc ggcagtcctg caccagttcg
     4201 cgctcaccct gcattacatg gatagacatc gccgtctgac catctttaaa ggtggtgaaa
     4261 tcctgagcgc gggccaccgg aatcgtggta ttacgcggaa tcactttctc caccaggccg
     4321 cccatcgttt cgaggcccag cgacagtggg atcacatcaa gcaacagcat ttcgctgtct
     4381 ggcttgttac ccaccagaat atccgcctga atcgccgcgc caatagcgac gactttatcc
     4441 gggtcgatgg aagtcagcgg tggacgaccg aaaaattcgc ctacccgttc acgcaccagc
     4501 ggcacgcgag tagaaccgcc caccatcacc acttccagca cttcatcagc ttctacaccc
     4561 gcgtctttca gcgcgcgacg acaagccagt aaggttcgtt ttaccagtgg cgcgatcagt
     4621 tcattgaatt gttcacggct gatttcgccc tgccagcccg caacgttaac ggtcacggag
     4681 tccgcatcgc tcagcgcgat tttggctgca atggcggcat ccagcagttc acgctgaacg
     4741 cggttatcgc tacgatcaga aatgcccgcc tgctcgcgaa tgtaatccgc cagcagatgg
     4801 tcgaaatcat cgccgccgag cgcggaatca ccgccggttg ccagcacttc aaacacgccg
     4861 cgacttaagc gcagaatgga aatatcaaac gtcccgccac cgaggtcata aacggcgatc
     4921 acgccttcct gaccggaatc cagcccgtag gcgatagccg cagcggtcgg ttcgttaagt
     4981 aagcgcagga cgtgaaggcc cgccagacgc gccgcgtctt tggtgccctg acgctgggca
     5041 tcgtcaaagt acgccggaac ggtgataact acaccatcca gctcgcctgc cagggcttca
     5101 gttgcccgcg ccgccagtgc tttgaggatg tccgcagaaa cgcgcaccgg gttcagcagc
     5161 cccgccgccg tttcaatcat cggcaggccg ttttcgctgg cctcgaattg ataaggcaga
     5221 tgcggatagc gttgctggat atcagccagc gagcgtccca tcaggcgttt aacagaacta
     5281 attgtgttgg cggtatcgag cgctgcatta gtacgcgcgt cataacccac cgaatgccct
     5341 tgctgttgat agtgaacaac agatggcagc aggtgacggc cttcatggtc ggctaacgtt
     5401 tcggcctgac cgctgcgcac tgtcgccacc agcgagttgg ttgtgcccag gtcaataccg
     5461 gccgccagac gacgctgatg cggcgcagca ctcaaaccag gttcactaat ttgtaataag
     5521 gccatgttta gcttccagaa attaaaaatc gagcagtttt tcttcgagtt gttcggcact
     5581 gcttcgcagt ttatcgagaa aacgcagctt acgcacggta tccgccgccg cgtcccacgt
     5641 ctcgttgtct aactgttcaa ccatcaactg atggcgggta tcaaacatct ttttcacacg
     5701 tttgataaag ctttccagcc gcgcttcatc tttcgcctgt tcgatctcgt ccagctcttc
     5761 gcgcagctcc aactgttcca tcaggaacgc ggtgtcgcgc acagtatgct gctcgctggc
     5821 gagatcaaag ccgtgcaaag aaagcaaata ttccgcgcgc attaacggat gacgcagcgt
     5881 ttgccaggcc tggttaatgg ttgcagattg ctgtacggcg gcgagttgtt ccgcctggct
     5941 tccgctggcg aatttatcag gatgatactg acgttgtaga tcctgaaaac gcaggctcag
     6001 cgcctgggta tcgagttgat aacgggcagg caagccaaag agggtgaagt aatccataac
     6061 attctcaggg ctgcgatccg ccgcgataac ggcgaatcag gtaaaacaaa ccccacgcgc
     6121 aggcgaccac ggtggggtta tcggtatgcg catcaaacgt ggaagctttc gccgcaacca
     6181 cactcatctt tgacgttcgg gttggtgaat ttaaaccctt cgttcaggcc ttcttttacg
     6241 aagtccagct gcgtaccgtc cagaaattgc aggctcttgc catcgaccac gactttcacg
     6301 cctttgtctt caaacacgat gtcttccggc atcggttcgt caacaaattc cagtacataa
     6361 gccatacctg aacatccgga ggttctcacg cccagacgca ggccaaaccc tttgccgcgg
     6421 ttagccagaa aggtatttac tcgcgctgct gcactgtcgc tcagtgtaat cgacataacc
     6481 aaacctcaac tcttattttg cttcacgttt gcttttatag tccgcaatgg cggctttgat
     6541 cgcgtcttct gccagaatag aacagtgaat tttcaccggc ggcagttcaa gttcttcagc
     6601 aatatcggtg tttttgatcg cctgcgcttc gtcgagagac ttccctttca cccattcggt
     6661 gaccagggag ctggaagcga tagcggaacc gcagccgtaa gttttaaaac gcgcgtcttc
     6721 aatgatacct tcatcgttga ctttaatctg caacttcatc acgtcgccac aggccggtgc
     6781 acccaccatg ccgctgccga cgttctcgtc gttgttgtca aaggaaccca cgttacgcgg
     6841 attctcgtaa tggtcgataa ctttttcgct gtaagccatt ataaattctc ctgattccga
     6901 taccgattaa tgatgagccc attcgatgct gttcagatcc acgccctgct tgtacatttc
     6961 ccacagcgga gaaaggtcac gcagacgacc gatggattta cgaactaact cgatggtgta
     7021 gtcgatctct tcttcagtag taaaacgacc taaagagaaa cggatagagc tatgtgccag
     7081 ctcgtcgttc agccccagcg cgcgcagcac gtaggacggt tcgaggcttg ctgacgtaca
     7141 ggcggaaccg gaagaaaccg cgagatcttt cagcgccata atcagcgact caccttcaac
     7201 gtagttgaag ctgacgttga gaatgttcgg cgcaccgtgt tccaggtcac cgttcaggta
     7261 aacttcttcg atatctttga tgccgttcca cagacggtta cgcaggccgc gcagacgttc
     7321 catctcggtc gccatctctt cttttgcgat gcgataggct tcgcccattc cgacgatctg
     7381 gtgaacaggc agagtgccgg aacgcatacc gcgttcgtga ccgccgccgt gcatttgcgc
     7441 ttcgatgcgt acgcgcggtt tacgacgcac atacagcgca ccgatacctt tcgggccata
     7501 gattttgtga ccggagaaag acatcaggtc aactttcaac tggctcaggt cgataggcag
     7561 tttgcccacg ctctgggttg catcaacgtg atagataatg ccacgagcac ggcacatttc
     7621 gccgatagcc gcaatatcct gtaccacgcc gatttcgtta ttcacgtgca tgatagaaac
     7681 gaggatggtg tcgtcacgca tcgccgcttc gagttctttc aggtcgataa taccgttacg
     7741 ctgcggtgcc aggtaggtga cttcgaaacc ttcgcgctcc agctgacggc aggtatccag
     7801 taccgctttg tgttcggttt tgctggtgat gatgtgcttg ccttttttct gataaaagtt
     7861 ggctgcacct ttgatcgcca ggttgtcaga ttcggttgca ccagaggtaa agacgatttc
     7921 acgcggatca gcgccgacca gatcggcaat ctgattacgg gcgatatcta ccgcttcttc
     7981 agcctgccag ccgaaacggt gagaacggga ggccgggtta ccaaaggttc cgtccatcgt
     8041 cataaactgc atcattttct cggcaacacg cgggtccacc ggcgtggttg cggagtagtc
     8101 gagataaatc ggtaatttca ttgctctata aactccgtac atcactcaat gcaaggaacc
     8161 aggctaccgg ctggatgtac gaccgtgttt acggagcgcg atacgctccg gcctgattct
     8221 gaatactttt tatcttttat gcgcgcaact taacgtcgat cgcgtcttgt gtgcgggtgc
     8281 gtggcgcgtc gtgagtatgc tgacgtccag acacatccag cacttcctgg ttattaacca
     8341 gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca
     8401 gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga caacgggtgg
     8461 catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg ctggcatctt
     8521 tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca tttttacgca
     8581 gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg gaaatatcag
     8641 ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg gtcacggcat
     8701 agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc ctgcccgggg
     8761 ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat tcccgagtaa
     8821 attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt gccatttttt
     8881 tatcgtggcg tttgtacctg ttgtcggatg cggcgtgaac gccatatccg acctggggtt
     8941 ctgtacactg taggcctgat aagacgcatt acgcgtcgca tcaggcaacg gctgttattc
     9001 ggccttgtta cctttattct gctgctcaat agaagccaga atcccgcgca ggatattcaa
     9061 ctcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca tcacctgccc
     9121 cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat gaccataaaa
     9181 acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct gctcgccatt
     9241 ttcctgagtt gccagccagg ccatacgcac ttcataggca ataacctgaa ccgccatcgc
     9301 caggttcagc gagctgtatt ccgggtttgc cgcaatcgcg acatgataat gacacttctg
     9361 caactcttca ttggtcaggc cgacacgttc gcgaccaaac accagcgcca ccggggtatt
     9421 tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg gccacggcag
     9481 cgtgcgggaa cgtgcgctgg tgcccaccac caggctacaa ccggctaacg cttcgtcgag
     9541 cgtatcgacg atgtgagcat taccaatcac atcgctggcc cccgccgcca gggcaatcgc
     9601 ctgggagtca ggtttcacca gtggattaac cagccacaga ttggttaatc ccattgtttt
     9661 catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg
     9721 aatattttgc agcattgtct ttcttcgtct aaagattatt cgcgcatctt atcataaaac
     9781 gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca
     9841 gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg
     9901 taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag
     9961 taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg
    10021 taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga
    10081 tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta tcaaacgtct
    10141 gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt
    10201 atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa
    10261 cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtaccattc tggcgaccgg
    10321 cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg gcaaactgtt
    10381 caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc
    10441 tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggact tcgctgcagg
    10501 cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg gtcataacta
    10561 catgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt gtgaaagcca tgctggcgaa
    10621 catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg tgatatcact
    10681 cacccgccct cgcctttcag gcgctattcc gaaatacttc ctcaccgctt tactttcttt
    10741 cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc aaggaaaggt
    10801 tatggcactg ccagtgaaca aacgcgttct caaaattctg tttattctct ttgttgttgc
    10861 cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc ctgacgataa
    10921 aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta aggacggtac
    10981 tcgtctgcaa ggctggttta tcccttcttc gacgggccct gctgacaacg ccatcgcaac
    11041 catcattcat gctcacggca atgctggaaa tatgtccgcc cactggccgc tggtcagttg
    11101 gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa
    11161 aggcacgccg tcccaggccg gattgctgga cgatacgcaa agcgccatca atgtggtgcg
    11221 ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta ttggcggggc
    11281 gaatattctt gctgttattg gtcagggtga tcgtgaaggc atacgtgcgg tgatcctcga
    11341 ctccacattt gcctcttatg cgaccatcgc caaccagatg atccccggca gtggctactt
    11401 acttgatgag agttacagcg gcgaaaatta catcgccagc gtcagcccga tcccgctttt
    11461 actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag
    11521 cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt
    11581 ttccgatcgt cacggcgatg tttatcgcga acagatggtg gactttatcc ttagcgcgtt
    11641 gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag
    11701 acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga cgcttgccaa
    11761 catttctgat gattagcatt cccttcgcca tttccctgag caaactttag ctattcttat
    11821 caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt
    11881 cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg ttgaccgcca
    11941 ccatggcaac cttcagcgag cttaatggtg tggatgatga tattgccagt cttgatatca
    12001 gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact ggttatgacg
    12061 gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac
    12121 gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca attcaccccc gccctgaaga
    12181 gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt
    12241 tagtgaatct ctgctcccga cggctgcaaa aacgctttga atcgcgcgat attcatttcc
    12301 tgtgtctgta tctgcaatat tgtttgctgc agcaccacgc tggaattacg cctcagttta
    12361 atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagca caggaaattg
    12421 gacgccactg gcagcgtcgg gcgctccagc ctgtaccacc tgatgagcca ctgtttatgg
    12481 cactactttt ttccatgttg cgggttcccg atccattgcg ggatgcgcat cagcgggaca
    12541 gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc
    12601 gtttttatga tgaacagggg ttatgtgatc agctttatac ccacctcgcc caggcgttaa
    12661 atcgcagttt gtttgccatc ggtattgata ataccctacc ggaagagttc gccagactgt
    12721 atccacgcct ggtacgcacc acccgcgcgg cgctggccgg atttgaaagt gaatacggcg
    12781 tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc
    12841 aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat agcgagcgag
    12901 aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac attaaacata
    12961 tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg attattgctc
    13021 cttataccat gccgttaccg ctcttttcac caccgctgat ctatacggac ctgacactga
    13081 caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc
    13141 gggcgcaaaa acattgccgg aagcgccacc agcgccatca cccagaacac gccgtggccc
    13201 agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg
    13261 acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc ggcaatatag
    13321 cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg caccactatc
    13381 aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc gcaaatcgcc
    13441 gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagtgcaaag
    13501 ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga ggccgagtag
    13561 ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgcgc cccctgcaat
    13621 aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca cgctgaccaa
    13681 ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag
    13741 ccgagcagca tggatgccac gcccaacgtc aacagcacga ggatcacccg ataatcaaac
    13801 atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga gccccacagt
    13861 cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc
    13921 aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat cagccacgct
    13981 acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc
    14041 aaggcggaaa tcaggcgaga aggatcgctg acgcggggcg cgatgagcaa actcccgaga
    14101 aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt taaaccaatc
    14161 cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa
    14221 tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg
    14281 ttagtctgga gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct
    14341 tcccgatgtc cgtaacgcca gttcacaagc actcaaaaac tgcgatccgt gacgcatgtt
    14401 gcaaagataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac aatggcgatg
    14461 aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa agcaatggaa
    14521 ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt attcatataa
    14581 ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac cagaatattc
    14641 gttgccacct ggacgatatt tggctggctg ttttcttgtg caaaccacgc tttaacgatc
    14701 ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc atccagttgc
    14761 gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac caccactaat
    14821 ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg gcgcattaag
    14881 ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat taaactcacc
    14941 agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa
    15001 ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg aacaattttc
    15061 cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa
    15121 cattctcccg ccgccgtcaa cgcgacttta cgcttatccc tcaccagtaa cggaacaccg
    15181 acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt atgcagtttt
    15241 tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg taaatgccgt
    15301 agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa attaactatt
    15361 tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag gtcaatacat
    15421 cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt taccaactga
    15481 ttgacaccca aaacggtcgg gtcactccgc gtatttatac cgacccggac atttaccaac
    15541 tggagcttga gcgtattttc ggtcgttgct ggttatttct cgcccacgaa agccagatcc
    15601 caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcagttgtc gtggtgcgcc
    15661 agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc atgcgtgtga
    15721 gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc tggtcttatg
    15781 gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca caagggttgt
    15841 gtaaatccca ctgggggcta aacgaagttc cttgtgtgga gagttataaa gggctgattt
    15901 ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac attgcctggt
    15961 atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa
    16021 agtgggtgat caactgcaac tggaaattcc cggcagagca gttcgccagt gaccagtatc
    16081 atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat gatggcagcg
    16141 ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc
    16201 tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa ccggatgcta
    16261 atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatacc gaagcagaac
    16321 aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatatt ttccccacgc
    16381 tttcatggct caacggcact gccacgctcc gcgtctggca tccgcgcggc cctgatcaag
    16441 tcgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt aaagccgctt
    16501 ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag gatgactcgg
    16561 agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac agcaaactgt
    16621 gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct ggcattacta
    16681 actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc gatcttctga
    16741 gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag gaggtgatga
    16801 aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc tctttcacga
    16861 agccagctta ctggacgact ggaaatttcg tgactggctg gcacagctcg acgaagagat
    16921 tcgttacacc atgcgcacca cggtgaacgc gcaaacgcgc gaccgccgca aaggtgttca
    16981 gccgccgaca acctggattt ttaatgacac caaagaccag ctggagcggc gaatcgcccg
    17041 tctggaaacg ggcatggcat gggcagaaga gccgccgtca cgcacccgcc acttaatcag
    17101 caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg tgaattatct
    17161 gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc gttttgacaa
    17221 agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg tcctggatca
    17281 agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat ttatgcgtgt
    17341 cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc
    17401 ttgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca tggtaatgcg
    17461 tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct acacgccgcc
    17521 agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact
    17581 tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc gcagccatga
    17641 gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta gggctggcgc
    17701 tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg tcggcggcga
    17761 aagtcgccag tctgcgtcag cgatttggtg aacatattct ggcggtggaa ggcaacgtga
    17821 cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg
    17881 attgttttat cggcaatgcg ggcatctggg atcacaatgc ctcactggtt aatactcccg
    17941 cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc ggttacctgc
    18001 tgggtgcaaa agcctgcgct ccggcgttaa tcgccggtga aggcagcatg attttcacac
    18061 tatcaaatgc cgcctggtat cccggcggcg gtggcccgct gtacaccgcc agtaaacatg
    18121 ccgcaaccgg gcttattcgc caactggctt atgaactggc tccgaaagta cgggtgaatg
    18181 gcgtcggccc ttgtggtatg gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg
    18241 aaacctcaat aatgcagtct ctgacgccgg agaaaattgc cgccatttta ccgctgcaat
    18301 ttttcccgca accggctgat tttacgggtc cgtatgtgat gttggcatcg cggcgcaata
    18361 atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcaatt cgcggcattc
    18421 gccacgtagc ggctgggttg gatctttaag gaagtacgat gaaagaaaaa acgatcatta
    18481 ttgtcggggg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag caagggttca
    18541 ccggtgagct gcatctgttt tccgatgagc aacatcttcc ttatgaacgc cctccgctct
    18601 cgaaatccat gttgctggaa gattccccac agttgcagtc tgtgttaccc gctcactggt
    18661 ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacattg ggccgcgaca
    18721 cacgagagtt agtgttagcc aacggcgaaa gctggcactg ggatcagctt tttatagcaa
    18781 ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc tgctttaccc
    18841 tgcgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa cggtcagtcg
    18901 tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg cagcgcagat
    18961 gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg
    19021 tgcaacgcta tcttttacag cgccatcagc aggctggtgt gcgcattctg ctcaataatg
    19081 ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt ggcgaaacgc
    19141 ttcaggccga tgtggtgatt tacggtattg gtatcagcgc caacgaccaa ctggctcgcg
    19201 aggccaacct tgatacggta aatggtattg tcattgacga ggcttgccgc acctgcgatc
    19261 ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt gcactacacc
    19321 gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgct gcaatgttag
    19381 ggctaccgct tccgctactg tcgccgccgt ggttctggag cgatcagtac agtgataact
    19441 tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac ccggaaaccc
    19501 agaaagcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggta acgctgaatc
    19561 agggacgtga gattcgctca atccgcaaat ggatccagag cggcaaaacg tttgatgcga
    19621 aacagctgac agatgagaac atcgcgctta aatcactgta accaggataa ttagcgaata
    19681 tctcaacgcc tggggcgtgg cgaggtacca gagtgtgtat tacgtttaaa tcacattatc
    19741 ttgcaaaggg attggttatg aactcactac gttattttga ttttggtgct gcccgccccg
    19801 ttttgttatt aattgcccgt atcgccgtgg tcctaatttt cattattttt ggttttccca
    19861 aaatgatggg ctttgacggt acggtccaat atatggcctc gctaggcgca ccgatgccga
    19921 tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg
    19981 gctttttcac ccgtccgctg gcggtactgt ttattttcta cacgctgggt acggcggtga
    20041 ttggtcacca ttactgggat atgaccggcg atgccgtcgg gccaaatatg attaatttct
    20101 ggaagaatgt cagtatcgct ggcgcgttct tgctattggc aattaccggg ccgggggcaa
    20161 tttctctcga tcgccgttag gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt
    20221 ttataaatta cagccactct acccgcaacg acatctctga agttgttgat tcccctggtg
    20281 caagcgcaat gaggtcacca ccttccgggc gatgatggtc atctggcgca tggctcatcg
    20341 gctccagaca gaagaaatca aacgcatatc ctttatcaaa cgccgggtcg gaaacaaaga
    20401 tgaaataaca cggtgcaggt ggcgtcgttt ctatgatgat ggcataaccc tcctgcggct
    20461 gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac tggcgcggca
    20521 acggcgcgag ctggttaaaa tccagttctt gcggtagctg ctcgcaaaac tcacccgcca
    20581 gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg
    20641 acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc ccttgattgg
    20701 tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga cttacccgat
    20761 agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg ctgcacgagg
    20821 cacattgcca ctgccccagc cagccgtcgc cgtgcagata gtgcgcatcc cactcaacat
    20881 tcggttgcag ttgatactca cgcccctgcc agacaaaatg gttgccgctc acccggtttg
    20941 caaacggcac tagcggaaaa caagaggcat cggttgccac accgcttttt ttaccaggac
    21001 gtaagagcgg cgtcgtatca cgccagaaac cttcgatgac accgccctgg tcagagacat
    21061 ccagctttag cggtccgtga gataaggtat agatggtcat ccgctaactc cttaatccgg
    21121 gaagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg catcacctgc
    21181 ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagcttcc agtcagtcaa
    21241 atcgtgggcg catttttcca tatggaacag actggtcacc caggagccaa taatccgccg
    21301 ttgatggtgc atcagatcgg cgctgacctc gaattccact tttccggttt caccaatgta
    21361 aaccacccgc ccccagtcag cagtggattg cagtgccagc aagcgacctg cggcattacc
    21421 ggaacaatcg agcgcaacat ccgcgccacc gtgggtaagt tcagcaatta tctgcggcag
    21481 accttcggtg gtcgctaaat agccgtgatc catcacccct aactgttttg ccatcgccag
    21541 acgttccggc agcatatcaa cgccgatgat ccgttttgca ccgcgacctt tcgccagcat
    21601 catcgccatc atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac
    21661 ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc
    21721 ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc
    21781 ggcatgaccg ccgtcacgct gccagccgta agccgctttt ccttcgccag tacaagaaat
    21841 aggaaaaccg cgacggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac
    21901 gcggtcgccc tctttaaaat ggcggcagcc ttgccccatc gccacaattt gcccgcacgg
    21961 ctcatgaccg ttgataaagc cctggtataa cggtttatcg ggcgccgccg ccgtggcgcg
    22021 gtgttgatgg tagatatagt ggacatcgct tccgcaaatc ccggaggatt tcattttgat
    22081 cagtacctgg ttaatccccg gcgtcggcac cgcaacttcc cgcagatcga cggtcgaatt
    22141 tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat
    22201 tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgcta
    22261 cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgcaccacc
    22321 tgctggaaaa aggagttaat cccgagcagc accagaccgt taccgattag cgtaatcacc
    22381 agcacaccaa gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg
    22441 accacgaccg cggcgatgac gtcaaactcc agaccgtttg ccgcacctgc gttaccggaa
    22501 ccgaggcgcg ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag
    22561 ataagaatgc gtacccgacg aacgttaata ccgcacaact gcgccgccgt ggcattaccg
    22621 ccaacggcaa aaaccgagcg cccgaaggcg gttttgcggc taatgaacac aaacagcgca
    22681 aacagcacca tcatgatcag cgcggatacc ggcacaccga gaaattgtcc gcccagccag
    22741 tccagcacct cgttttcgtc aatcggcact ggcagtgcgt tggtcataaa cagccccatt
    22801 ccgcgcaggg cgctccacag ccccagagtg gccacgaaac ttggcacgtt aaacacgcca
    22861 cgcagcaccc cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca
    22921 atcgccaacg gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc
    22981 atcggcccaa cgctgacatc aatttcaccg gagataataa tcagcgtcat cgcccaggcg
    23041 gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag
    23101 ccaggcgcgt tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc
    23161 tcattaatat ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt
    23221 aatgacgaag cagacatctt aggttcctcg gtaaaattca tcagtgcaca gacagaatgg
    23281 cggacatcag ctcatccaca ttgaccggag cgtgaaactc ctgcgagaac gtaccgtgct
    23341 gtaacaacag aatgcggtcg cacaccagcg gtaactcctc cacttcactg gagataaaca
    23401 ccacgctttt tccttcggca gccagctcac ggacaatacg gtaaatctgc tgtttggctt
    23461 cgatatcgac gccgcgcgtt ggctcgtcga gcaacaaaat ctggctggcg gcatagaccc
    23521 aacgaccgat cactactttt tgctgattgc cgccagaaag cgtgccgatg ggcgtttcgc
    23581 tactggcggc cttgaccgtc atccgctgca tcacttcttc ggtcaggcgg cggatggtgg
    23641 accattgcag cactccgttg gcgctgattt tttgccgatt ggtcagcact gtattttcgt
    23701 caacgcccaa ccagggaatg atccccgctt ctttgcggtt ttctggcgta tagccaatgc
    23761 cgcgtttcag catgtcgccg taatcggggc gcgtgatttt ctcgccgttg ataacaattt
    23821 cgccctgttc atacgtctcc agcccaacaa tcgccttcag caattcactg cgccctgccc
    23881 ccagcagacc agcaatgccg agcacttcgc cacaacgtag cgtaaaactg atatcctcca
    23941 gcttgggctt atggcgtaac gcacggactt ccagcacggc ctgatccaca atttcctgag
    24001 gtgcaaccgg cgcaatatca acgtgatcgc gccctagcat tagcgacaca atatgatgcg
    24061 tggaagtgtt ttcgagcacc acatcgccag ccacctgccc gtcgcgcata acggtggcac
    24121 aggaggcaat gcggcgaatt tcttccatcc ggtggctgac ataaatcacc gccacgccca
    24181 gtgccgacat ctttttcacc gcgctgatca ccagttcaac ttccgcactg gcaagcgaac
    24241 tggtaggttc atcaagaatg accacgcgcg gctcgccctt catcacccgc gcaatttcca
    24301 ccagctgctt atgcgccggg cttagtgttg aaacaagttg ttcaggacta acgtcaacgc
    24361 ccagcgcctg taagcaacgt tgggcatcct gcgccatttg caggtaatca atcatgccgt
    24421 tgcggcgggg ccactgaccg aggcagaggt tttccgccac cgtcagccct tccaccagac
    24481 ttaactcctg ataaaccgcg cgtaccccca gttcagcggc acggcgagtc agcgtagctt
    24541 cgtcgccttc cagtcgcgtc tcgccaatcc agatatcacc gctatccgga cgttcactgc
    24601 cggtaagcat tcgaatgagg gtcgatttgc ccgcgccgtt tttgcctaac agcgcacgaa
    24661 cctcgccttt attgagcgtg aagttaacgt tatccaacgc aacgacgccg ggataacgct
    24721 tatttcctgc caccactttt gctaccggga ctgcctctgt tgccgtgaac atagggacct
    24781 ctgcgaatca gcgattaggg cagaccatca acgtgcgttg ccagccactg tttaccgtct
    24841 tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta
    24901 ttgataacct tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc
    24961 actaccgctt tcagcacctg attgttttcc agctcctgag caatttcggt tgtcatatcc
    25021 gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg
    25081 agtgtcgcac cgcccgattc ccccataatg gcgttgagat ccggcgtgga gataatcagt
    25141 ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctgattagc gacaatttgc
    25201 gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg cacacaaact
    25261 tcaaaggctt cgcaattgat gacggcaatt ttcggctggt caattttatt ggcaataaaa
    25321 taatcggcgg cagcgttacc cagtttttta ccaaattcca gcgggtcacc caccagatac
    25381 gccgagacat atttatcgac ccccttttga ttaatacagg tgttgtagca aatcaccgga
    25441 atacccgctt cactggcgcg acgaacggta cggctactgc cattttcaga cactgccgat
    25501 aaaataatag catcgacatt acgcgccacg agggtatcaa caaaggtact ttctttcgaa
    25561 atatcaccct gggcgttagt ttcaattaac tgtacctgta ctgaagaatc ttttgccgca
    25621 tcctgaacgc cctgacgcac tccagcgtaa tatccctgag tatcaaggta tattgcgcca
    25681 atggtcattt ctttatccgc agctctggca aatagtgcgc tacctaatag cgtagccatt
    25741 aataatagat tacgggttgt tctcattttt ttaggcatag agcctcctgt agggttttta
    25801 ttaacaacgg cttattctaa ttattttgtg atgagcggca gcgcgtgccg ctcaaatatt
    25861 acagttaata gggtttagtg aacgtagtta aaaataaaag gcatcacggt ggtgaataat
    25921 gccgctttcg gccaggccgg attaatcgcc gtcaattcag cgcgagcaga ttcgtattgc
    25981 gcaatctcgc ctaatcccgc ggccgccagc atcgccacca tcaggcattt ttctttatgc
    26041 tgctgttgta aatcgccata cagcgacaac aagtcaggct gcgagacggc aaagaaatcc
    26101 gcttcgatac tggttttcgc catctcttgc gcccactgtt tcatttcact aaacagttgc
    26161 tgtgcgattt gttgttcgcc cagcagtcgc agcgccattc cttgccagaa gagataatca
    26221 accggctgat cgttgtaata actgtgaatg ttaatggtgc gatcgccagt cgccgccaga
    26281 cgtaaacaac gcgtcgcttc agtttcatcg ccctgcgcgt tggcgcatat cgcctgccag
    26341 aaccagatgt cgttatcagt ttgccccggt aaacggcctt cgcttaaatt ctccggataa
    26401 tgcagcgcgg catgaagcag ttcgctggcc tgctgcggct ctctggcatc aagatgctgc
    26461 caggcgcgta ataactggtt gaggataaac tgactggtga ccttcccttc cccgccttcc
    26521 cacgggtgga atttgcgcgt ggcgagaatg tccgccgctt tgtctgcctg ccccgtgaga
    26581 tgccacaaat tgagcagttc tgcggtcatg tcgtcgcgtt tcagcgcaat ttccagatta
    26641 ttttccagac gcgccagtcg tttctccggt gtggctccac ttaacttatc aagcaaatcc
    26701 cgttcgaaaa gcagacgtgc atcctgcggc gcaagctgat aagcattatc aagataacgc
    26761 gcggccagct cataatcgtg ttgcttattc cacgcatgga tcgctaaccc gcgccagccg
    26821 tcggcaaact ccggcgacat ctctacgcaa cgttgccata aggcaatggc tttgttgtag
    26881 ctacgtttgt tgtagtagaa gcaggccagt aaatggcgag caaaccagca ctcttcaata
    26941 ctctccagcg ccgccacttc ttccagcgta ttcgggaaac ggacaaactg cgggaagaca
    27001 tcaatggctt ttgcgaccag ttcgccacgt tcggctttcg gcagcaagct ggcttgcagg
    27061 taaagcggca gcgtgcgctg gcagtccagt gcgttcagca tctctgccgc cagggtgggc
    27121 attccccagt taatcagttg cccggcggtc atcagggcgt taacgtcgcg tccctgacac
    27181 agaccgcgcc actgcgcgag agctgattcg ctacgaccat cgaaccagtt cagccaccac
    27241 agagtggcgt tcagcggata atcgcgcagc agtttctcgc gctgcaaacg cgcgttgtcc
    27301 tgacgaccac ttaacaccag cagcagatta tgcaggcaaa gcacttcctg attggttggg
    27361 caggcgcgaa gactttgttg gcaaaaatcc agacccgcgt cgaagttacc attacgcgcc
    27421 gccagtcgtg ccagaccata atagcctccg gctttactgt tgccgctcca gaccgcacgc
    27481 cagaaatcct cttcggctag ttgatattgt ccctgacgtt cgtaagcact ggcgcgaatc
    27541 aaactcgcct gtccgcactg cggatttttg ttcagcccat gtgcgcgttt cagagcctga
    27601 gtggcatacg ccaccgcttg cgggaaatct gcgcggttat actccagcat cgccagcgcc
    27661 agattacagc ggtaatccag cggatccagc gccacgccgc gcaggtagta atcgaacggt
    27721 gaacggctgg cgtgatgata ttgctccaga tgctgaccga taaaccaggc ttcatctgta
    27781 ctggtaatgt cttgtgctgc cagtggcgct ttggcgacgt ccggcaacgg caactcttgc
    27841 ggctgatgtt cctgataact cagtacaata ttgccgtcgg catcggagag ctcaatggtc
    27901 agcctttcag gattgatacc gtgcaacacg ccctggatgg cggtcgcagg catcagtgcc
    27961 acggcatcat caagtaacgc gttgcatttg ccgatttcgc ggatcgccag gcggtatccg
    28021 ttcaacggag agatggcata cagcccccac tcaatccccc gctcactacg ctggagtttt
    28081 atcaccgcat cgcgggaggc attttgcacc atgcccaaag aatgataagg caggaaatac
    28141 tgctcaaaac gcttctcttc gtaagcatca agccaggtaa aatcgggctg gttatcggca
    28201 aaaataccgg tcatcagttc gatatacggg ccgttattat cagttaggct cttatcccac
    28261 gcctggccaa attcactgtg tccccagctc cactgctttt tacctggcgc aatatggtgg
    28321 ttggcaacgt gtagcaaacc accatcttca tcgtgacacc acgcgccaac aaaatcgtac
    28381 tgcgattttt cggccatata tgaggttgga acgggcacat ttttatagcg agaaatgtcc
    28441 actccagcgg agtagtccac tttgtagtaa gtgccggtgg cgatggggaa agcggagacg
    28501 gcccgtttgc cgtgatcaaa caccgcagtc acatccggcg ggaaaacgct ctgatgccct
    28561 tcaccccctt tcactgccgg gttggcccac cacaagaaat gacgcggtgt ggcgttgccg
    28621 ttatagacgc ggctggcgat ttccagcgcc gcccggtcag ggcgcagggt gaaacctgtc
    28681 atcacctgta aaccatgcat cggctccgtt tcgcctaccc acaccgtctg tgtgccgtct
    28741 tcatgggctt cgagggtgaa atcaacgggc ataaaggtgg tcgggcgatg gtgttgcggc
    28801 cagttaaact caatcccgcc ggagatccac ggtcccagca gccccaccag cgcaggttta
    28861 atgacttcat tgtgatagac aaaatcgcgc tgtttcactt tatcccatgc gcgatgcacc
    28921 cgaccgccca attccggcag gatcatcact ttgatgtagt cgttttccag ccacaccgcc
    28981 tgccaggatt tcagggtttt ctgctcgctc agcgtatcgg tcacgccgta gggataaacc
    29041 gcgccggacg atccctgata aacgcgattt tccaggaaca tgggatgtat atcctgcggc
    29101 ccggtttcat aggtcgggat ctcaacgcgc tcttgccaca cttttactgg agtcatggtg
    29161 ccctcaatat taacaagaca tactgaatta aaagatttgt ggcagtgtat tgaacaatct
    29221 ggcaatgttt tcgcggaata atcacgcaat taactaaaca aggtttagtg aagatgagag
    29281 cctgcattaa caatcaacag attcgccacc ataacaaatg cgtgattctg gaactgctgt
    29341 accggcaaaa gcgcgccaat aaatcaacgc tggcccggct ggcgcaaatt tcgattccgg
    29401 cagtcagtaa tattttgcag gaactggaaa gcgaaaaacg ggtggtgaat atcgacgatg
    29461 aaagccagac gcgcgggcat agtagcggta catggctgat tgcgccggaa ggtgactgga
    29521 cgctgtgtct gaacgtgacg cccaccagta ttgagtgtca ggttgctaat gcttgtttaa
    29581 gtccgaaagg tgaatttgag tatttacaga ttgatgcacc gacgccgcag gcgctgctgt
    29641 ccgaaatcga aaaatgctgg catcgccacc gtaaattgtg gccggaccgc accatcaatc
    29701 tggcgctggc aatccacggt caggttgatc ctgtgaccgg cgtgtcgcaa accatgccgc
    29761 aagcgccgtg gacaacgccg gtggaggtga aatatctgct ggaagaaaag ctgggcattc
    29821 gggtgatggt cgataatgac tgcgtgatgc tggcgctggc ggaaaaatgg caaaataatt
    29881 cgcaggaacg ggatttctgc gtgatcaacg ttgattacgg cattggctcg tcgttcgtga
    29941 ttaacgagca aatttatcgc ggcagtttgt atggtagcgg acagattggt cacaccatcg
    30001 ttaatccgga tggcgtcgtc tgcgactgtg gacgttacgg ctgcctggaa accgtcgcct
    30061 cgttaagtgc tttaaaaaaa caggcgcggg tatggctaaa atcacaaccg gttaatactc
    30121 agcttgatcc tgaaaaactg actacagcgc agttaatcgc tgcctggcag agtggagaac
    30181 cgtggatcac cagctgggtt gaccgcagtg ccaatgccat tggtttgagt ctgtataact
    30241 tcctcaacat cctcaatatt aatcagattt ggttgtacgg tcgcagttgt gcctttggtg
    30301 agcactggct taataccatt attcgccaga caggatttaa cccgttcgac cgcgacgaag
    30361 gaccgagcgt gaaagcgacg caaattggct tcgggcaatt aagccgcgca caacaggtgc
    30421 tgggaattgg ctatttgtat gttgagacgc agttacgaca gatttgatgg cgcgataacg
    30481 tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc ttatgcgtaa
    30541 accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat aacggcttca
    30601 tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc ttctgcttct
    30661 ttgaagccgc gacgcgtaat tgccggagta cccacgcgaa taccggaggt cacaaacggg
    30721 ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg gcccagagcg
    30781 gcgtctgctt ctttaccggt caggttttta tcgaccagat caaccaggaa cagatggtta
    30841 tcagtaccgc cggaaaccac tttgtagccg cgttccagga acacttctac catcgcttta
    30901 gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc ttctttcaga
    30961 gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc agggaaaacg
    31021 gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat caggccgccg
    31081 cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg aggaaccggg
    31141 ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac gaacaggtaa
    31201 gcgccgatgc tgtcagcgat ttcacgcatt ttcgcccagt caaccacgcc ggaatatgca
    31261 gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc cagatcggcg
    31321 tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag tttaccggag
    31381 aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat acccagaacg
    31441 gtatcacctg gttccagcag cgcggtgtag accgcaaagt tagcctggga gccggagtgc
    31501 ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat cgccagttgt
    31561 tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata accttcagca
    31621 tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt gtagttttcg
    31681 gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat agcctgccac
    31741 agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat ctcctgactc
    31801 agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg cgaacagtat
    31861 aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac gattaccttc
    31921 aggctgcgca aggctttgga gaataaagag cttgcaaccg gaaacggatt tcttttcagg
    31981 tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa agaaccattt
    32041 acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag atgcaaaaaa
    32101 aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc atccctttac
    32161 tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt actcataacc
    32221 cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa cgtgaagccc
    32281 tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg ctgctgccag
    32341 cggtagaaaa aatcgcgcag aagcacacca gtttccagat caaaccggaa cagtacaaca
    32401 tcgtcggtga acacctgttg gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc
    32461 tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat cgcgaggcgg
    32521 aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc gatttccgca
    32581 ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag ccggtcgacg
    32641 gtggcgcagt gacagaatac cgtccggggc aatatctcgg cgtctggctg aagccggaag
    32701 gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat ggcaaaggct
    32761 atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg cacaatcacg
    32821 ccaatgttgg cgatgtcgtg aaactggtcg ctccggcagg tgatttcttt atggctgtcg
    32881 cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca atgctggcaa
    32941 tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc catgcggcag
    33001 aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag tcactgccgc
    33061 gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct aaaggtcagt
    33121 ttgatagcga aggtctgatg gatttgagca aactggaagg tgcgttcagc gatccgacaa
    33181 tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa cagttagtgg
    33241 atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat aaggtgctgt
    33301 gatttgatgt tgccggatgg aaacatccgg caacccctga cgcggtttaa attgccgcgt
    33361 cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg aagattttac
    33421 cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag gtatcgacaa
    33481 tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc atatactccg
    33541 cgccgcggta cagctcggta tggcctttct ggcggccaaa acctttcacc tcagtgaccg
    33601 tcatgccggt aatgccgact tcagccagtg cttcgcggac atcgtccagc ttgaagggtt
    33661 taataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct
    33721 gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc agctcgtgtc
    33781 gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga
    33841 cgttgccttt ggtaatttgc agcagcttac gcaaatagtt gagttcaaac tgattacgtg
    33901 cctcaacaaa ggttggcagc gccgtgtttt cgccctccag cgcctgctct actaatgcat
    33961 cactaatcac cggagatgag gtcagcgcca cgcactgttc aatcacgttg accaactggc
    34021 gcacattacc cggccagctc gcggtcatca ggcgtttcat tgcatcggtg gagaacgcgc
    34081 ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa
    34141 tgtcttctgt gcgctctgcc agagccggaa ttttcaggct gacaacgtta aggcggtaat
    34201 agagatcttc acggaattcc ccgcgcgcca tcgcttttgg caggtcgcgg tgagtggcag
    34261 aaataatccg cacgtcgata tcaatatcgc ggttactgcc cagcgggcgc actttacgct
    34321 cctgcaacac gcgcagcagt ttgacctgca acggtgcggg catatcgcca atctcatcga
    34381 gaaatagcgt accgccttcc gccgcctgga ataaaccttc gcgattgctg acagcgccag
    34441 taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg ggcaatgcgc
    34501 cacagttaat agcaataaat ggtttgctat tgcgcgggct ggcgttgtgg atagcctggg
    34561 cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg tctgattgcg
    34621 ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg acaatcgcct
    34681 cgcgccagcg ttcgtcggtg gctggcgcgg attgttccag cgcatcatca attgcctgat
    34741 atagcgcgtc tttatcaaca ggcttggtga ggaaactaaa aacgccctgc tgtgttgcag
    34801 caacggcatc gggaatagaa ccatgcgcgg taagaataat tactggcatt cccggctgca
    34861 ctttctggat ttcagcaaac agctgcatac cgtccatttc atccatccgc aggtcgctga
    34921 tgactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca ctttccgccg
    34981 tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc aatcccggat
    35041 cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac ctcatgggtg
    35101 gatggcgcgg gcttttcacc ctcgtgcggc gtatccggcg agaaatttcc agccggtttg
    35161 cgagttgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt ggtgagttcc
    35221 agctgctgtt gtagaacgtg atgttgctgg cgcaaagtat ccagctcgct gtcgctggat
    35281 tgctggagtt tgccgtagcg ttggcgctct tccgccagtt gcagttgcag cgcctgaccg
    35341 tcgcgccaga gttgatatag aggccgaacc tgtgccggga tctcggtact tagcgcatca
    35401 atgcgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc
    35461 ccttgcttaa aggtattttg ccagctgccg ttgtcatatt ggcgggcttg ctgacgcgac
    35521 tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag cggattggtt
    35581 tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag atagtcagcc
    35641 agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt attgtgattc
    35701 tgcacgcaac ccagcaatgc cagacatggc agtcctgcca gccacagccg tcggggcaat
    35761 aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag tgggcgtaat
    35821 tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat gtcgtacgtg
    35881 aggatttcga gcactgcccg ggtccgaact ggcaaataaa atagcctgat gggataggct
    35941 gttagcatta tttcgtgttt ttcgacgacg gcaattcaat gcggaaacaa acgtcttgcc
    36001 cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc ctggcaatgc
    36061 ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg
    36121 gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa
    36181 tataaacccg cgcaccatgt aaactgctgc gaaggcaaat gttaccggat tcagccccgt
    36241 agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt ggctccgcca
    36301 ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt gcgggcaggc
    36361 tatgagcaga aactactgtc tccaccagcg gtgctaactc aacattatct agttccaccg
    36421 cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt tgcaaattgc
    36481 ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa
    36541 cctgatcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt tttaattcat
    36601 gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg cgctcactta
    36661 accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac gaaacgctat
    36721 tgcccagaga acgcccttcc cccaaccgat tgatcatccg ttcaatattt ttcaccggcc
    36781 cgataatcat ccgcgtgaaa agcagtacca ttaccagact caccagaaat agcaccagcg
    36841 attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag
    36901 agaacactac tgtgcgcgtg gcctgtacca tttcggtatt ggcactggca aaggcttcca
    36961 gacgcgcggc agcagcagca tcgggaccac tgttgttgca ctgaagttgg gccagattgt
    37021 tcaagtcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct gcgtgggcat
    37081 cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc gtagggtcat
    37141 ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg gtcattgctt
    37201 cgctgcgccg ggcatcgata agcgtggtgc ggttgaccag cgccgcctgg tcgctaagcg
    37261 cattcaggct ttgccaggct tgccatgcca gcaccaacag gggcagcaga atcagcaaaa
    37321 atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt ttcaaggtgt
    37381 tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga tacaacaaag
    37441 ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg tttcgcccga
    37501 tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt tgtgcgtcat
    37561 tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg agccactggt
    37621 cactattatg cagtaaatgt gccaataaag aaaatagttt ggtaacgtac tgattatacg
    37681 ctgctttgag gggtttatgg cttcttcgct atttgaatgt aaatcagcca ctgtgtcgct
    37741 aaaaagagac aacttaagat aaacttatta gatgacatta aaatcaatga gttaaatgtc
    37801 accaaaaagc gacacagcaa accacccatt gtcgtgattt acagacacaa aaaagctccc
    37861 tgagttggga gcttataata gcggttggta tttaatgccg gatgcggcgt gaatgcctca
    37921 tccgggctgc aaaaccaatg ggctgacgac ttaccccaac tgcttacgtg cattgcggaa
    37981 aatgcgcatc catgggccat cctcgcccca gttttcagga tgccaggagt tgctgacagt
    38041 acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccgcttt cagtcgtgac
    38101 cgccgtaata ccgttcggag aaccgttcgg gttagccggg taggtttcag tgactttgcc
    38161 gaagttatcg acatagcgca gtgccaccag ccctttgctt tccagtgccg ccagatgcgt
    38221 cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca tttgcgagcc
    38281 caccatcccc tgcaacagca gcgacgggct ttgggtcact tcaaccaggc tgaaacgcgc
    38341 ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac ctgggatcag
    38401 ttcacgcaga ttagacatca tctggcaacc gttacatacc cccagcgcca gcgtttgcgg
    38461 acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga tagacttcgc
    38521 ccaaccttca ccggcaccca gcacatcacc gtaggagaaa cctccgcacg cgaccagggc
    38581 gtgaaaatcc tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca catcgattgc
    38641 atcgaagcct gcacggtgga aggctgccgc catttcaaca tgtgagttca caccctgctc
    38701 acgcagcacg gcaactttcg gacgtgcgcc agtggcaata tacggtgctg ccacatcttc
    38761 gttgatatcg aacgacagtt tcacattcag gccaggatcg gcgtcgtttg atttcgcctg
    38821 gtgctccttg tcggcacact ccgggttgtc acgcagccgc tgcatctgcc aggtagtttc
    38881 tgcccaccag acacgcaacg tagtgcggct ttcgctgaat acagtctgcc cgttggcggt
    38941 aatcacaaaa cggtcaccgg aaaccgcctg cccaacataa tggacgcaat ccgcaagccc
    39001 atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct gaatcaccgc
    39061 acccaattct tcgttaaaca acgccgccag gcgatcgtca cccagagtgg cgatatccgc
    39121 gtcaatgcca caatgaccag caaaggccat ttccgccagc gttaccagca ggccgccatc
    39181 agagcggtcg tgatacgcca gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata
    39241 gaagcctttc agttgcgcga catcgcgtac atctgccggt ttgtcgccaa gctgacgata
    39301 aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat caatcagcag
    39361 cagtgcgtta tcttcggtag aaagctgcgg cgtgatggtg tgacgtacat cttccacgcg
    39421 ggcaaatgca gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt cgttaccttc
    39481 ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtca ggcccagcgc
    39541 cggacaaagc tcttcgccca cggctttaac ggcttcatac aggcccgcat cttcaccagg
    39601 gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat cgccaatctg
    39661 tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg cggcgaaatc
    39721 cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt agctgtcgag
    39781 gctggcggta gtgacagcgc agttagcgac cggcacctgc cacggcccca ccatctgatc
    39841 gcgcgccacc atgccggtta cgctgcggtc accgatggtc accaggaaag ttttctccgc
    39901 cacggtcggc agatgcagta cgcgtttcac cgcgtctgca atggtgatcc cttcacggac
    39961 cagcgcatcg cccttcgctt tcagcgtttg tacatcgcgg gtcatcttcg gcgttttacc
    40021 aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat cgtgcagaga
    40081 aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgct cacgcttaca
    40141 cagttcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc gttcctggga
    40201 ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa tatcgcgcag
    40261 ttcaaattta ccgccgcgcc cgccgtcgct caccagttcc ggcatcgcgt tagaaagacc
    40321 gccagcgccg acgtcgtgga taaacaggat tgggttggca tcaccaagct gccagcaacg
    40381 gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta cggaagcaaa
    40441 gtcgaggtcg gcatcagact gacctgacgc catggaagac gccgctccgc cgccaagacc
    40501 gatgttcatt gccgggccgc cgagaacgac cagtttcgca ccgacgttga tctcgccttt
    40561 ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg gtttgtgata
    40621 accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac ggaagtagcc
    40681 attcagtgcc ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg ggccttcggt
    40741 catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt cccacggctg
    40801 ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg ctttcggctt
    40861 cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccagaaccgg tcgccgcgcc
    40921 cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca gaatatgcgc
    40981 cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc
    41041 agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtgat ctggcgtggt
    41101 ttcgaaagta tttttgatca tcttgaacag cgatttcggc tgctgttcac cgtcgataat
    41161 ccagtcggca ttaaaaattt tgtggcggca gtgctcagag ttcgcctgag cgaacatata
    41221 cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca gatagtcaat
    41281 ttcatcttcc gccagagcca ggccaagacg caggttagcg tcgatcagcg cctgacggcc
    41341 ctgccccagc aaatcaacgc tggtaaccgg agtcggttga tggtgggcaa acaactgctc
    41401 tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac
    41461 ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc
    41521 aaggcggttt acctgttgta gcccgcaatt atgggcaata tcggtcgctt tcgaagacca
    41581 gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg gggcgtggct
    41641 ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa
    41701 cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa ccgggagcct
    41761 ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg caggcgaacc
    41821 acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc caaggggggg
    41881 gaaacgggcg tcattataaa gaatctgatg cgctgacgaa accgtttgcg tggaaataaa
    41941 atcaccatcg tgaattagca acgcgtgccg ccaatggctg taataagttg ccatctggcg
    42001 caggtttacg caaaatgccg ctcatttatg agtaaacctt tcactattat tacgtttttt
    42061 caagctggga cgcgcacgac acagagaatt aactaattga aaaaattaaa gattaattat
    42121 ctgttcatcg gcattctggc actgctgctc gcggtcgctc tctggccatc cattccctgg
    42181 tttggtaaag ccgacaaccg tatcgccgcc attcaagcgc ggggagagtt gcgtgtgagc
    42241 accattcata ctcccctgac ttataacgaa atcaacggga aaccttttgg cctggattac
    42301 gaactggcga aacagtttgc cgattacctc ggtgtaaaac tgaaagtgac cgtgcggcag
    42361 aatatcagcc agctgtttga cgacctcgat aatggtaacg ccgacttgct ggcggcagga
    42421 cttgtctata acagtgagcg ggtaaaaaat tatcagcctg gccctaccta ttattccgtg
    42481 tcacaacaac tggtttataa agtgggtcag tatcgcccac gtacgctggg caacctgacg
    42541 gcggagcaac tcaccgttgc accgggtcat gtggtggtta acgatctcca gaccctgaaa
    42601 gaaacaaaat tcccggaatt aagctggaag gtagacgaca aaaaaggctc tgcggaatta
    42661 atggaagatg tcatcgaagg aaaactcgat tacaccattg ctgattctgt cgccatcagc
    42721 ctgtttcagc gcgttcaccc ggagctcgcc gtagcgctcg atatcaccga tgaacaaccg
    42781 gtgacttggt ttagcccgtt agatggcgat aatacccttt ccgccgccct actcgacttc
    42841 ttcaacgaaa tgaatgaaga cggtacgctg gcacgcattg aagagaaata cctggggcat
    42901 ggcgatgatt ttgattacgt cgatacgcgc acatttttac gcgccgtcga tgcggtactg
    42961 ccgcagttaa agcccctgtt tgagaaatac gccgaagaaa ttgactggcg tttgctggcc
    43021 gctattgctt atcaggaatc gcactgggat gcacaggcca cttcaccgac gggtgtgcgc
    43081 ggcatgatga tgttaaccaa aaataccgcg caaagcctcg gcattacgga tcgtaccgat
    43141 gccgaacaga gcatcagcgg tggcgtgcgt tatttgcagg atatgatgag taaagtgccg
    43201 gaaagtgtgc cggagaacga gcggatctgg tttgccctcg ctgcgtacaa tatgggctat
    43261 gcgcatatgc tggatgcccg cgccctgacg gcaaaaacca aagggaatcc tgacagttgg
    43321 gctgacgtaa aacagcgtct gcctttactt agccagaaac cctattacag caagctgact
    43381 tacggctacg ctcgtgggca tgaagcctac gcttatgtcg aaaatattcg taagtatcag
    43441 attagcctgg tgggttatct gcaagagaaa gagaagcagg ctacagaagc ggcgatgcaa
    43501 ctggcgcagg attatccggc ggtatcgcct acggagttgg gcaaagagaa atttcctttt
    43561 ctctcgtttc tttcccagtc gtcatcaaac tatttgaccc attctccctc tctgctgttt
    43621 tccaggaaag ggagtgaaga gaaacaaaat taatccgtcg aggattgcgc ttttttctgc
    43681 gctttaattt cctggcggcg catgcgaaag aagtcactga gcaacgccgc gcactcatcc
    43741 gccagtattc cttccgtaat ttccactcgg tgattcatac ccggatgatg cagcacatcc
    43801 attaaagatc ccgcagcgcc agttttcgcg tcacgcgcac caaagaccac gcgaccaatg
    43861 cgactgtgga tcatcgctcc ggcacacatt acacatggtt caagcgtgac atacaacgtg
    43921 gcgtcgatca gacgataatt ttgcatcacc agaccgccct gcctcagggc catgatttct
    43981 gcatgtgcgg tgggatcatg tcgaccaatc ggacggttcc agccttcgcc aataacacga
    44041 ttgttatgca ctaataccgc gccgaccggt acttcccgct catcccaggc acgcttcgcc
    44101 agcgtcattg catgacgcat ccagtattcg tggctaaatt cgacttcaga caaaaagaaa
    44161 actccggtta taaaagcgcg gcgcattata cacggacgct atgctttact ctattccagt
    44221 tgctggagtt caccgcgcgg ggttactcgc caacgatgct gacagaaata aagcagcgga
    44281 ttgtcctgat tactgtcgct atagccactg tacagccgca gcggcgtgcc gattttgcgc
    44341 tccagttgtg cgaccttttc atgtcccaga caacgcatcg tcaataccca accaccatag
    44401 ccacgctgaa tttggctggc gataagatta actcgcggca gccagggcgt atcgaaataa
    44461 accgcttcaa ccagcggctg cggagagccg gtaatcaacc agatatcagc atcagaactt
    44521 aacaggtagg tggttaatcg ctcctgaacc agcggaaagg cggtaacatt gtcacgaaac
    44581 cagcgtacga aatcggcctg caacgcctgc aaacgcgctt cattgtgacc aaaagtgcat
    44641 ccccacagaa gcagactcat cggccagcgt gccgcacgac cttttatcaa taacgcaatg
    44701 gctataatcg gcaacaacgg caggacaagt aacgcattca gcggttggcg acgcagtaaa
    44761 tagcgcagaa aactgccgaa catatcctgc tgatgcaatg ttccatctaa gtcaaaaaac
    44821 accacacgac gctcgtgagt tgccaaacca tactcctctg gactgaaaca tcctgattaa
    44881 ttttcgctac tacatagcct aacagataga tcatcacttt tccggcaaca atcgaccgca
    44941 tcatggctaa ctggaatttt taattcatgg caattagcgg caatggaata taaaattcac
    45001 tcgcgtgtgt ctcatattgc ctgttgtcgc cactatttca tcgcgaggaa agatatgaac
    45061 ggcttacttc gtatccgtca gcgttaccag gggcttgccc agagtgataa aaaactggcg
    45121 gattatctgc tgctacaacc tgatacggcg cgccatttaa gctctcagca actggccaac
    45181 gaagccggag tcagtcagtc cagcgtcgtg aagttcgcgc aaaaactcgg ctataaaggt
    45241 tttccggcgc ttaagttggc gttgagtgaa gcgctggcaa gccagccgga atcgccctca
    45301 gtgcccattc ataaccaaat ccgcggtgat gatccgttac ggctggtcgg cgaaaaactg
    45361 attaaagaaa atactgccgc tatgtacgca acgctaaacg ttaatagtga agagaaactg
    45421 catgaatgcg taacaatgtt gcgctctgcg cggcggataa ttctgaccgg tattggcgct
    45481 tcgggtctgg tggcgcaaaa ctttgcctgg aagctgatga agattggctt caatgctgcc
    45541 gcagtgcgcg atatgcatgc gctactcgct accgtacagg cgtcgtcccc tgacgatctg
    45601 ttattagcca tttcttacac cggtgtacga cgcgagttaa acctggcggc agatgagatg
    45661 ctgcgagtgg gcggaaaagt gctggcgatt accggcttta ctcccaatgc cctgcaacag
    45721 cgtgcttctc attgcctgta taccattgcc gaagaacaag cgacaaacag tgcttcaatc
    45781 tctgcttgtc acgcgcaggg aatgttaacg gatttgctgt tcattgcgct gattcagcag
    45841 gatctggaac tggcaccaga acgtattcgt catagtgaag cgctggtgaa aaaactggtc
    45901 tgagtaaaga atgcgcgtat aatgcccgcc cggtttgtgt tgttttgaga gtttccttat
    45961 ggcgctgtta atcactaaaa aatgcatcaa ttgtgatatg tgtgaacccg aatgcccgaa
    46021 tgaggcgatt tcaatgggag atcatatcta tgagattaac agcgataagt gtaccgaatg
    46081 cgtagggcac tacgagacac caacctgcca gaaggtgtgc ccgatcccca atactattgt
    46141 gaaagatccg caacatgtcg agacagaaga acagttgtgg gataaatttg tgctgatgca
    46201 ccacgcggat aaaatttaat attctactct ggaagtagag tattaattat tttactggga
    46261 agccttaacg ccattatatt tatttaattg atgacattag cataatcatt cactaagtta
    46321 atttatatag tatctgccca gacacttatt tatagttatt aaaggcgcgt ccgattggtt
    46381 caccggacgc accttaagta cgtttccttg tgttataaga acagaaggat cagctgtaaa
    46441 acagcaatga tgattttgat ggcccgttta ataaggtatc ggcaatcagt cattcgtttt
    46501 tccttaaaca aggaatgcag ccgttcagat tagcccttac atctccccaa aactgaacgt
    46561 gcgagttatt gagggtgcat gctgcactcc acaccagagc tttgacgaca ccactcgttt
    46621 caatggggga attctgtggc atggtgtaaa gcacagcaaa atcgccaata acgaaaccaa
    46681 ttttaatgta cttacgaatt ggcggtcacg ttaatcttgc catcaatatc gctttcttcg
    46741 taaaggctcg agtttttatg ataaagattg caagttgctt gtaaaagata actaccctga
    46801 tccataatcg ctgttgttga gggtgcgtgc tgcactaaat taaagttaca aagtaaaacc
    46861 cccgttcctt accagttcgg gggttttact ttttaaagag aacggtatta tttttaactt
    46921 tcaataatta ccgtggcgca agcatagtgg cgctcatcgg ccagcgtcac atgcatattt
    46981 gcaacgccca gcttttccgc cagttttaat gcctcgcccc acagtcgcag ccgtggctta
    47041 ccaagctcat cgttaaatac ttcaaactga ttaaacgcca gaccattgcg gatcccggtg
    47101 ccaaacgctt ttgctgcggc ttcttttacg gcaaagcgtt tcgccagaaa acgcaccggc
    47161 tggtggtgcg ttttccaaat ttcccattcg ttatcgctta atacgcggcg tgccaggcgc
    47221 tcaccggatc gggcgatcac tgcttctatg cgagcgatct cgacgatatc cgtgcctaaa
    47281 cctaatattg ccattagcca cgcgcttcca gcatcaggcg cttcatttct gccaccgcat
    47341 ctttcagtcc ggtcatcact gcacgaccaa taatggcatg accgatattc agttcgtgca
    47401 tctcagggat ggcggcaatg gcttttacgt tgtgataggt cagaccgtgt ccggcgttaa
    47461 ctttcaaacc gagactagcg gcaaaggtcg cggctttggc gatacgcgcc agctcttgcg
    47521 cctgttcggc gtcagttttg gcatcagcat agcaaccggt gtggatctcg ataaacggtg
    47581 cgccaacctc tgccgcagct ttgatctgct cttcatcggc gtcgataaac agagaaacct
    47641 gaatcccggc atccgccaga cgtttgcagg catcgcgcat tttgtcacgc tgccctgcaa
    47701 cgtccaggcc gccttcggtt gttacttcct gacgcttttc tggtaccagg cagcaaaaat
    47761 gcggcttcgt ctcaaccgcg atcgccagca tctcttcggt caccgccatc tccagattca
    47821 tgcgggtatc cagcgtctga cgcaggatgc gcacatcgcg gtcagtaatg tggcggcgat
    47881 cttcacgtaa atgcacggta atgccgtccg cccccgcctg ctcggcaata aacgcggcct
    47941 gcaccggatc cgggtaagcg gtaccgcgcg cgttgcgcag cgtagcgata tggtcaatgt
    48001 tgacgcctaa cagtaattca gccatgacaa tcctcatcat tcataatgtg ttttcaccgt
    48061 tcgcttaggc ataaattgcc ggaacagttc cctgctcttt aaaggtttac cgccaagata
    48121 cggcttaagc gccatgcggg taaagcgttt cgcggcgcgc agtgtgtctg cgtcaggaaa
    48181 ttcccgtgcg tttaacgctt ttaactgcct tccggtgaac gttttattgt cgataacgac
    48241 gcttgcgata aacccttttt cttcgcgata acgatacgtc atggtgtcat ctaccggctc
    48301 gccgctaccc gcacaatggg taaaattgac gccataaccc agatgcccga gcagtgccag
    48361 ttcaaagcgg cgcagcgcgg gttctggcgt accagtgccc cctgcaagag actgaatgca
    48421 gtgcaagtaa tcgaaaaaga gttcagagaa gcgcgtctcg tattccagta cgcgggagag
    48481 aagttcgttg atgtacagac cgctgtaaag cgtgataccg cttaatggca gcgccagcga
    48541 gacagcttca gcactgcgca gcgttttgac ttcgccacgc ccgccgaaac gtagcaagag
    48601 aggggtgaaa ggctgtaatg cacctttcag ggtagagcgt ttagagcatg cgcctttcgc
    48661 aaccagacgc acgcgacccg attcctccgt gaagacgtcc agcatcaggc tggtttcgct
    48721 ccacgggcga ctatgcagga caaatgcgcg ctgccagcct tccatcggag ttactcttaa
    48781 agatcgtcaa cgtaaccgag actgcgcagt gcgcgttcgt cgtcggccca accggatttc
    48841 acttttaccc acagctcaag gtgaacaggc gcttcaaaca tttcctgcat gtctttacgc
    48901 gcttcaatcc cgatggtttt gatcttggcc cctttgttgc caatgaccat cttcttctgc
    48961 ccttcacgct caacgagaat caaaccgttg atgtcataac caccgcgttc gttagagacg
    49021 aaacgttcga tctccacggt cacggagtac ggcagttcag cgccgaggaa acgcatcagt
    49081 ttttcgcgga tgatttcaga cgccataaaa cgctgtgagc gatcggtgat gtaatcttcc
    49141 gggaagtgat gagtcgcttc aggtagatgc ttacgcacga ttgccgcaat agtgtcaaca
    49201 ttcagcccgg tttcggcaga gattggcacg atatcgagga agttcatctg gcttgccagg
    49261 aactgcaggt gcggcagcag atcggctttc tcctgcacgt tgtccacttt gttcaccgcg
    49321 aggattaccg gcgctttgcc ttcgcgcagt ttgttgagca ccatttcgtc gtccggcgtc
    49381 cagcgggtgc cttcaacgac aaaaatcacc agctcaacat cgccaataga gctgctcgcc
    49441 gctttgttca tcaggcggtt aatggcgcgt ttttcttcca tatgcaggcc cggtgtatcg
    49501 acgtagatcg cctgatacgc gccttcagta tggatcccca caatgcggtg acgagttgtc
    49561 tgcgccttgc gggaggtgat ggagattttc tgccccagca atttattcaa caatgtggat
    49621 ttgccaacgt tcggacgtcc gacgatggca ataaatccgc agtaactttt atcgatgctc
    49681 attccagctc cagttttttc aacgcctgtt cggcggcagc ctgctcagcc ttacgacggc
    49741 ttgaacctgt gccaaccacc ggttcactca ggccgctgac ctggcagtgg attgtaaatt
    49801 cctgatcgtg cgcttcgcca cgtacctgga ctaccagata agtcggcagc ggcagatgac
    49861 gaccctgcaa atattcttgc aagcgcgttt tcggatcttt ttgtttatcg cctgggctaa
    49921 tttcgtccag acgagtttga taccagttga ggattaattt ctcgacggtt tgaatatcac
    49981 tgtcgaggaa tacgccacca attaatgctt cgacggtgtc ggcgagaatt gactcacgac
    50041 gaaatccacc gcttttaagt tcacctggtc ctaaacgtaa gcactcgcct aactcaaatt
    50101 cgcgcgccag ttccgccagc gtattgccac ggaccagcgt ggcgcgcatc cggctcatat
    50161 cgccttcatc cacacgaggg aaacggtgat aaagcgcatt ggcgataacg tagctcagaa
    50221 tagagtcgcc taaaaattct aaacgctcgt tatgtttgct gctggcacta cgatgagtta
    50281 atgcctgctg caacagttcc tgatgattaa aagtgtagcc cagcttccgt tgaagccgat
    50341 taattacgat ggggttcatg cgataccaat aaataaatgc gtcaacaatt cagcacacga
    50401 aacagacctg atatacatgg ctctgctaac tgcttcgctg cagtttcagt ttagcggtat
    50461 atggggacca acgctgtttc gtgtgccgtg gcaacctgga ggtgccaacc ttaaacttcg
    50521 ggggaatatt ctatacacaa cgacggggga tgtcgttagc cacgggagat ttatctcata
    50581 aataattcac gttgtcgcca taacggcgac aacgtgaacg aagatgagct attaatggat
    50641 gccgccaatg cgacttaagc gcacacctgt cggccattcg ccttcttgct tatcgaagct
    50701 catccagata gccgttgccc gaccgaccag attcgcttca ggcacaaagc cccagtaacg
    50761 gctgtccgcg ctgttgtcgc ggttgtcgcc catcatgaag tattgtcccg gaggaacaat
    50821 ccaggttgcc agttgttgcc ctggctgctg gtaatacatc cccacctgat cctgcgcaat
    50881 cggcactgtc agaatgcggt gcgtcacatc acccagtgtc tctttacgct cggaaagacg
    50941 aattccattt tctttggttt cgtttttcgg cacttcaaag aatccgctgg tcgcttcccc
    51001 accattacgg cgtgagaagg tctgaacgaa atcgctcggt tccacgtttg agtaggtgac
    51061 cggcagcgcg ttttcacacg cctggccgga actgcatccc ggttgaatcg tcagctcttt
    51121 tgagaccgga tcgtaagtga ctttatcgcc cggtaaaccc accgcgcgct tgatgtaatc
    51181 aagctttgga tcttccggat atttaaagac cacgatatcg ccgcgtttcg gatgaccggt
    51241 ttcgatcagc gttttctggt agataggatc tttaatgcca taagcaaact tctctaccag
    51301 aataaaatca ccgattaaca gagtcggcat catcgaacct gacggaatct ggaacggttc
    51361 ataaataaac gaacgcacaa tcaatacgat agccagtacc ggaaaaacag aagcaccggt
    51421 ttccagccag ccaggcttcg gcgcaacctt tttcaacgtt gctttatcca gtgagtcacc
    51481 ggcagccgcc tgcgccgctg cctgacgttc ccgccgttta ggtgcgaaaa agaatttatc
    51541 cacgcaccat aaaatgcccg tcaccagtgt ggcaatcacc agaatcaggg caaacatatt
    51601 cgccatgcca actcctaagg gttatttgtt gtctttgccg acgtgcagaa tggcgaggaa
    51661 cgcttcctgc ggcagctcga cgttaccgat ctgcttcatg cgtttcttac cttctttctg
    51721 cttctgcagc agctttttct tacggctgat atcgccgcca taacatttag ccagtacgtt
    51781 tttacgcagc tgtttcacgg tggatcgcgc aataatgtgc gtaccaatcg ctgcctgaat
    51841 ggcgatatca aactgctggc gtgggatcag atctttcatc ttctccacca actcgcgacc
    51901 gcggttttgc gaattatcac ggtgggtgat caacgccagc gcatcaacac gttcaccgtt
    51961 gattaatacg tctacacgta ccatgtcgga cgcttggaag cgtttgaagt tgtaatccag
    52021 agacgcataa ccacgcgagg tagatttcag gcgatcgaag aagtcgagca ccacttccgc
    52081 catcgggatc tcgtacgtca gcgccacctg attaccgtgg taaaccatat tggtctgcac
    52141 gccgcgtttt tctacgcaca gcgtaataac gttgccgaga tatgcctgcg gcagcagcat
    52201 gtgacactct gcaatcggct cgcgcagttc gtaaatgtta tttaccgcag gcagcttgga
    52261 tgggctgtcg acgtagataa cttcccttga cgtggtttca acttcataca ctacggtcgg
    52321 cgcagtggtg atcagatcca gatcgtattc acgttccaga cgctcctgga tgatctccat
    52381 gtgcagcagg ccaaggaagc cgcagcggaa accaaagccc agcgcgcttg agctttccgg
    52441 ctcatagaac agtgaggcat cgttcaggct gagtttaccc agcgcgtcac ggaaggcctc
    52501 atagtcgtcg gaacttaccg ggaacagacc ggcgtatacc tgcggtttga cttttttaaa
    52561 gccaggcagc gccttttctg ccggattacg cgccagcgtt aaggtatcgc cgactggagc
    52621 gccgtggata tctttaatcg cacatacgag ccagcctact tcgccacatt tcagttcagt
    52681 gcggtcaacc tgtttcggcg tgaagatgcc cagacggtcg gcgttatagg tctgcccggt
    52741 actcatgact ttcactttgt cgcccttacg cagggtgccg tttttaatac ggataagtga
    52801 aacaacgccc aggtagttgt cgaaccatga gtcgataatt agtgcctgca acgggccttc
    52861 cggatcgcct tccggcggcg gaatgtcgcg caccagacgt tcgagaacgt cctgcacgcc
    52921 aacgccggtt ttcgctgaac agcgcaccgc gtcagtggcg tcgatgccga cgatatcttc
    52981 aatttcttcc gccacgcgtt caggatcggc tgccggcagg tcaatcttgt tcagtatcgg
    53041 caccacttcg agatccattt ccatggcggt gtagcagttt gccagggttt gcgcttccac
    53101 gccctgcccg gcgtcgacca ccagcaatgc accttcacag gcagccagcg aacgggaaac
    53161 ttcataggag aagtctacgt ggcccggggt gtcgataaag ttaagctgat aggtttcgcc
    53221 gtcagacgct ttgtagtcca gcgtcacgct ttgcgctttg atggtaatgc cacgctcacg
    53281 ctcaagatcc atggaatcga gaacctgcgc ctccatttca cggtcagaca ggccaccgca
    53341 gatctggata atacggtcag acagcgtcga tttaccgtgg tcaatgtgag ctatgatcga
    53401 aaagttacgt atattcttca taaagtatga ttattgtgcc ttaatgcccg gttaaccagg
    53461 cttttagaag tcgctgttct gagcttaacg tctgtattaa tagaaacgcc gcattctaca
    53521 ctacaacatt gaggcgagga aatgttcata ccgtatggat tgtggtatct ggaaacgtcc
    53581 tcgcatttgt tatgcaaaat gcaacaaagc cagtgaaatc actggctcgc gtcttccgaa
    53641 gatgtttcaa atcgcacaag gccaggcggc aatgccacgc ttaagatgat cggttgccat
    53701 tctgcccggg cagcaaactt acgcgagtag ccacgcgcaa tcaggaaccc gccaataccg
    53761 ccgaggaccg caccacataa tgctgcgacg tcagaagcaa agagtagctg aaatagcgaa
    53821 gcgatgagga ataatcccac cagcggcgac atataaacca gtaatgcgga gctaagcagg
    53881 ctgccttcgg cgatccccaa ttccactttt tgccccggca ctaacggctc atcacagggt
    53941 acgacaatgg tatgcgtggt ttgcgggcca agtttattca acacgcggct accgcaaccg
    54001 gcgcgtgaag cgcagctgct gcatgaggct ttaacatcac aactgaccag cgcctgcccg
    54061 ttttgccagg agacgacggt agcccactct ttgatcattg cgctgccccg aacttaatat
    54121 tctcggcaat gcgtttcgcc gtttgcggcg gcagttcacc gacaatggtg atttcggcgt
    54181 tatcacgtac gcttgtactg acggttctgc gtccggtgcg caacatctga tcggtgctcg
    54241 atggcgtagc gcggttaacg tttaccgaga agctgaataa tccgtcggaa tagagacgtg
    54301 attcgatagg catgttgtcc atcgtcggta gcggacgtcg actactggaa acttcgctaa
    54361 aaccctgtgg caaccaggtg ggagtccagc tgaatttggc tttttcacct acaggaacag
    54421 aaagcaacgg cggcaaattt gccttcgcca gcgtctgcat actgctgctg atatcctgat
    54481 tgacattaaa agcaatcacg cgaaattgtt ccagcgtttc accatcgcga tcaaggaggt
    54541 caacccgcat cggtaatttc gattcggtgt ccatccacac aatgtagctg tagcgtgtgc
    54601 catcacgagc aaccacgcga ataacttcac aaagacgatc agcaatacgc gtgcgcccga
    54661 cggagataaa gtcgtagtaa ggagaaaggc gtttgaaatc ggtatagatg agcgatggca
    54721 gagaatcaac aatgtaatcg ccattaagcg tgaacggttc aagtcccggt tcaaaatagc
    54781 tgatttcatt gccgcgctgt accacttccc ggcgcgggcc atccatttgc aacaattgtg
    54841 caagaggacg gttatcgagg cgtgcatgtc gataacgcag agactcaaca ccctgtttat
    54901 tgatgctgat gaatgacagc tcgtaattca gtgactgact ggccaggttc atctgctgta
    54961 ataacgcccc ggacgcggga gtggccgagg cgttagcaga gaataacagg ctacctgtca
    55021 ctaatgacat ggcaaaccaa agttgcttca ttactgcgat tgcgttccta aagtttgaat
    55081 tcctggcacc tgtacagcgg cttgctgggt ttgcgcctgc tcaaactgaa gctgttcaga
    55141 gtggagtcgg cgttgcagtt cgtaatcctg caacattgca ttaatgcgac gacgctgctc
    55201 ctgtacctgc tgctgttgac cattatttgc ggtcgcttca gaaggtactc ccaggctaac
    55261 cgggctggct ttacccatca tcggcagtgt attaaatacc ggcgtttcgg gctgctggga
    55321 cgtttcagat tgtccattat agtgctggac gccaacgata actgcaagcg atacgcatgc
    55381 ggctacgccc atttgggtaa gctgtgccgc ccacggacgt actttctgcc agaatggcat
    55441 tttctgccat tgatgcggcg caggctgggc ttccgggatc aatgtcgccg gttgacgtac
    55501 tggctcttct tcaatggcgg ccatcacgcg tgaagagata tcgaaatgga gcacctcggg
    55561 agtatcaccc cgcattgagt cacggattaa gtgatagctt tcccaggttt tctgcatttc
    55621 tgggttatga gccagttcgt taagcagctc actatccagc gtttcgccat ccattaaagc
    55681 ggaaagttgt tctttctgca tgcctaatac ccttatccag tatcccgcta tcgtcaacgc
    55741 ctgataagcg gttgaacttt gttatcaata gcttccctcg ctcggaagat acgtgaacgc
    55801 accgtaccta ccggacaatc catgatagcg gctatctctt catagctcag gccatccagc
    55861 tcccgcaagg ttattgccat gcgtaaatct tccgggaggg actcaatagt tcggaaaact
    55921 atctgtctca gttcttctga caacattaag ttctcagggt tcgaaatttc tttcaacgcg
    55981 ccgccacttt cgaagttttc agcttcaatg gcatccacat cactggaagg tggacgacgc
    56041 ccctgagcaa ccaggtaatt tttcgctgta tttacagcaa tccgatacag ccatgtataa
    56101 aaagcgctat ctccccggaa cgaatccagc gcacgatagg ctttaataaa agcttcttgt
    56161 accacatcgg gaacatcacc cgacggcaca tagcgggaaa ccagactcgc cactttatgc
    56221 tgatagcgca ctaccagtaa gttaaaggct ttctgatctc ccttctggac ccgttcaacc
    56281 aggacctggt ccgttaactg ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc
    56341 acgcgctatc gaaacgccac tccattagct gcaatttgag caagcaaagg gttagagtgt
    56401 ctcgtttttg taaagttccg taacgcatct gtttttgttt gtcatgctgt agacggatca
    56461 ttatctatca ttataagtct acagaatctg aacatcgcat tatctgtgta gaaatgccca
    56521 tttaactgcc tgaagagtaa cccaacggcc tttttatttc accacctaat cctccaccag
    56581 ccagtaactt ctctttttct cgccgccctg cgtcagcgtg tttagccact gtaacaaata
    56641 ttaaaatagc aggtgtttat tcgcacaaca tgatgctatg ctgaccaaac cgtgtttagt
    56701 aaattaaaca aagaaaatga atactctccc tgaacattca tgtgacgtgt tgattatcgg
    56761 tagcggcgca gccgggcttt cactggcgct acgcctggct gaccagcatc aggtcatcgt
    56821 tctaagtaaa ggcccggtaa cggaaggttc aacattttat gcccagggcg gtattgccgc
    56881 cgtgtttgat gaaactgaca gcattgactc gcatgtggaa gacacattga ttgccggggc
    56941 tggtatttgc gatcgccatg cagttgaatt tgtcgccagc aatgcacgat cctgtgtgca
    57001 atggctaatc gaccaggggg tgttatttga tacccacatt cagccgaatg gcgaagaaag
    57061 ctaccatctg acccgtgaag gtggacatag tcaccgtcgt attcttcatg ccgccgacgc
    57121 aactggtaga gaagtagaaa ccacgctggt gagcaaggcg ctgaaccatc cgaatattcg
    57181 cgtgctggag cgcaacaacg cggttgatct gattatttct gacaaaattg gcctgccggg
    57241 cacgcgacgg gttgttggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg
    57301 ccacgcaaaa gcggtggtac tggcaaccgg cggtgcgtcg aaggtttatc agtacaccac
    57361 caatccggat atttcttctg gcgatggcat tgctatggcg tggcgcgcag gctgccgggt
    57421 tgccaatctc gaatttaatc agttccaccc taccgcgctg gatcacccac aggcacgcaa
    57481 tttcctgtta acagaagcac tgcgcggcga aggcgcctat ctcaagcgcc cggatggcac
    57541 gcgttttatg cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg
    57601 cgccattgac catgaaatga aacgcctcgg cgcggattgt atgttccttg atatcagcca
    57661 taagcccgcc gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct
    57721 ggggattgat ctcacacaag aaccggtacc gattgtgcct gctgcacatt atacctgcgg
    57781 tggtgtaatg gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggtga
    57841 ggtgagttat accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg
    57901 tctggtctat ggctggtcgg cggcagaaga catcaccaga cgtatgcctt atgcccacgg
    57961 cgtcagtacg ttaccgccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt
    58021 aattcagcat aactggcacg agctacgtct gtttatgtgg gattacgttg gcattgtgcg
    58081 cacaacgaag cgcctggaac gtgccctgcg gcggataacc atgctccaac aagaaataga
    58141 cgaatattac gcccatttcc gcgtctcaaa taatttgctg gagctgcgta atctggtaca
    58201 ggttgccgag ttgattgttc gctgtgcgat gatgcgcaag gagagtcgtg gcctgcattt
    58261 tacgctggat tacccggaac tgctcacgca ttccggtcca tcgatccttt ctcccggcaa
    58321 tcattacata aacagataaa aagcctgggt tagcgccgta tacgcttcgg aatagttctg
    58381 gtctggccca cgaatgacta agcgatcgct aaagcattct cccgcctgcg gggagaatgc
    58441 cagcagcacc cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa
    58501 atgccagccc atacttaatg ccagctccgt aaaactatta ccaatctgct ctggcagcac
    58561 cacgcagaaa aatccctctt cggtaatgca ctccgccgca caggccagca acgatgggtg
    58621 atcaagcgta gtagtatagc gagcctgttt ccgttgaggt gtcgagcact ctactccctg
    58681 ctgatagtaa ggtgggttac tgatgattaa atcaaagcgc gcggtctgct gtgttaccca
    58741 ctgctgaata tccgccgtat ggacgttaat tctctctaac cacggggact gggcgacatt
    58801 ttcctgcgcc tgagaggcag cttcactttc cagctcaacg gcatcaatta tcacgctgtc
    58861 actggttcgc tgcgccagca tcagtgccag caagccacta cccgcaccga tatcaaggca
    58921 acgttttatc ccagccaccg gtgcccatgc gcccagcaat atgccatccg ttcccacttt
    58981 catcgcacag cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag
    59041 cacggatgta gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg
    59101 gtgagaacaa tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg
    59161 tctataatca gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg
    59221 aactcgacga aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca
    59281 ttcaggctgc cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga
    59341 caggcaccgg taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc
    59401 cgcgtaagaa atccggtccg ccgcgtattt tgatcctcac cccaactcgc gagctggcga
    59461 tgcaggtggc cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca
    59521 tcaccggcgg cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg
    59581 tggtcgccac gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg
    59641 cggttgaaac gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg
    59701 atatcgaaca tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa
    59761 ctctggaagg cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg
    59821 tttctgccaa tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg
    59881 atgacctgga acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc
    59941 gctcaattgt gtttgtgcgt aagcgtgagc gtgtgcatga gctggcaaac tggctgcgcg
    60001 aagcgggcat caacaactgc tatctcgaag gtgagatggt acagggcaag cgtaacgaag
    60061 cgatcaaacg tttgaccgaa ggtcgcgtaa acgtactggt tgcaaccgat gttgctgcgc
    60121 gcggtatcga cattcctgac gtcagccacg tctttaactt cgatatgccg cgcagtggcg
    60181 atacttattt gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcaattt
    60241 cgctggtaga agcccatgac catctgctgc tgggcaaagt tggtcgctat attgaagagc
    60301 caattaaagc tcgcgtaatt gatgagttac gcccgaaaac gcgtgcacca agcgagaagc
    60361 agaccggcaa gccatcgaag aaagtactgg caaaacgcgc cgagaagaaa aaagctaaag
    60421 agaaagagaa gccgcgggtg aaaaaacgcc atcgcgatac caaaaatatt ggtaagcgcc
    60481 gtaaaccaag cggaacgggc gtgccaccgc aaacgacaga agagtaatct caatgccggg
    60541 tttaagcccg gcattatttt tatcccggca aaaaacaatc ttcaacacac tgaataaaag
    60601 tgtgcatcgc cgggctaacc gcttttccgg catggtgagc gcacattgcc gtaatggtct
    60661 gcgagtgttc gccaaaaggc aattcaatta attctccgct ctcaagctct ttttccaccg
    60721 caaagcgggg caagtaactg acaccgatat tcgccgcgac acaacgtttg atactttcaa
    60781 tgcttaacag ctcaatggtg ttttccaccg tgatccgccg ctggcgcagc gtgctctcaa
    60841 atatctgccg gaatacacat tgcggttcgt tgataataaa gctacaggcg ttatgtcttc
    60901 ccggctcagt aaaatcgaca tctgcaattt gcggtgaagc taccagcacc agcgattgct
    60961 cacccaattc tcgtcggttc agggcatcat cattccctac gcgataaaaa accccgacat
    61021 cggcctcatc attcaatagc gcatcacgga tcacgtaaca gttcagagac tgcaacgata
    61081 aacgcacttt tggggcgcgt tgccgaaaac gctgcaacac ctgcggcatt cggtaagaga
    61141 gcaacgtttc gcccgaaaca acgcgtagtt ccccgtccgg atccgactcc tttttggcag
    61201 cctcacgaag ggtatccatc actctggtga gttcataaat gtacggcagc agctttttcc
    61261 cttcgcgggt aaggcacatt cgtcgaccaa ttttctcaaa taactgtacc gagaactcct
    61321 gctcaagctg ctgaatatga aaagtcaccg tcgattgtgt acagcacaat ttttgcgaag
    61381 ctcgcaaaaa ggaaccctct tccaccacgg ttttaagcgt aataaaacgg cgcagatcca
    61441 taaccccaaa cctatcgaaa atatcgaatt tagaatgtaa aaacattcat ttttttaaat
    61501 gttccgtgtc gggtactgtc taccaaaaca gaggagataa caagtgacac cgaccctttt
    61561 aagtgctttt tggacttaca ccctgattac cgccatgacg ccaggaccga acaatattct
    61621 cgcccttagc tcagccacaa cgcacggatt tcgtcagagt acccgcgtgc tggcagggat
    61681 gagtctggga tttttaatag tcatgttgct gtgtgcgggc atttcgtttt cactggctgt
    61741 tatcgacccg gcagcagtgc atctgttgag ttgggcagga gcggcatata ttgtctggct
    61801 ggcgtggaaa atcgctacca gtccaacaaa ggaagacggg cttcagacaa aaccaatcag
    61861 tttttgggcc agctttgctt tgcagtttgt gaacgtcaaa atcattttgt acggtgttac
    61921 ggcactgtcg acatttgttc tgccgcaaac acaggcgtta agctggatag ttggcgtcag
    61981 cgttttgctg gcaatgattg ggacgtttgg taatgtgtgc tgggcgctgg cggggcatct
    62041 gtttcagcga ttgtttcgcc agtatggtcg ccagttaaat atcgtgctgg cgctgctgct
    62101 gatctattgc gcagtacgca ttttctatta atgaaaaaaa gcggaagagg tcgccctctt
    62161 ccgcttagta acttgctact taagccttac aggctttcag taaaggtacg agcgataacg
    62221 tcgcgctgct gttccggagt cagagagtta aagcgaactg cataaccgga tacacggatg
    62281 gtcagctgcg gatatttttc cggatgctta actgcatctt ccagagtttc acgacgcaga
    62341 acgttaacgt tcaggtgctg accaccttca acgcgaactt ctggtttcac ttctactgga
    62401 acttcacggt actcaatgtc acccagtttg cttactgcaa ccacttcgtc ttctgcaaaa
    62461 cctgctttcg caacgatgca acgtgcttcg cctttttcgc tgtccagcag ccagaaagag
    62521 ttcagcagat cgtcgttagc ggctttagta atctggatac ctgtaatcat gtgatgcctc
    62581 cccggcaaaa ttatttgatt tgttcagcct gtcgcggcca attggtaaaa ccattgttgc
    62641 ttgagtgtat atatactcct caaacaccct tgattctttg atttaaatca ataaaaacca
    62701 cacatcgatt gtggtcgcaa atggatttta ttgttttaca tcaacttatg cgggtgtgaa
    62761 attttaccaa tttacatttt tttgcactcg tttaagtcta aaaaatgagc atgattttgt
    62821 tctgtagaaa gaagcagtta agctaggcgg attgaagatt cgcaggagag cgagatggct
    62881 aacgaattaa cctggcatga cgtgctggct gaagagaagc agcaacccta ttttcttaat
    62941 acccttcaga ccgtcgccag cgagcggcag tccggcgtca ctatctatcc accacaaaaa
    63001 gatgtcttta acgcgttccg ctttaccgag ttgggtgacg ttaaagtggt gattctcggc
    63061 caggatcctt atcacggacc gggacaggcg catgggctgg cattttccgt tcgccccgga
    63121 atcgccactc ctccgtcact gctgaatatg tataaagagc tggaaaatac tattccgggc
    63181 ttcacccgcc ctaatcatgg ttatcttgaa agctgggcgc gtcagggcgt tctgctactc
    63241 aatactgtgt tgacggtacg cgcaggtcag gcgcattccc acgccagcct cggctgggaa
    63301 accttcaccg ataaagtgat cagcctgatt aaccagcatc gcgaaggcgt ggtgtttttg
    63361 ttgtggggat cgcatgcgca aaagaaaggg gcgattatag ataagcaacg ccatcacgta
    63421 ctgaaagcac cgcatccgtc accgctttct gcgcatcgtg gattctttgg ctgtaaccat
    63481 tttgtgctgg caaatcagtg gctggaacaa cgtggcgaga cgccgattga ctggatgcca
    63541 gtattaccgg cagagagtga gtaaatttgc gggaaaatgc cggatggcag agttgtcacc
    63601 cggctgattt atcaggcttt attctgacgc caccattcac caagcaaaac gcctgttgca
    63661 acagagatat tcagcccggc aacgttgccc gtaccgtcaa tcttcacgcg cagatcgttc
    63721 ggatcgcgtg cggcatccgg taacccttca tattcctgac ccagcaccag taccattttc
    63781 gctggcagac tggttttgaa cagcggttta ccctgctcgc tggaagttgt cactacggtg
    63841 taacccgcct gacggaaatc atccagcacg ttaacaatgt tgtcgccagt aatcggctga
    63901 acgtgctctg cgccgccttc tgcggtacgg atagccgcac ccgactccag cagtgccgca
    63961 tcctgcacca caacgccttt cacgccgaag tgcgcgcagc tgcgcatcat gccacccagg
    64021 ttatgcgggt tagattcgtt ttccagtgcc agaacgcaat cctgcgcacc tgcctggctt
    64081 acccactgct gcacggttgt gccattacgc tttttgatca ggaagcaaac gccaccgtga
    64141 tgttccgtgc ctgacgcttt tgtcaattcc gcttcatcca ccacatggta cgctttgcgg
    64201 tttgccgcca tccagcgcaa ggcttcttta aaacgtggcg ttacgctctg gataaaccag
    64261 gcgcgaacaa tcgcttccgg acggctctgg aacagtgcct gacatgcatt ttcgccgtag
    64321 acgcgggttt cttccgcacg ctgacgacgc aaaacttctg gatcaataaa acttttacca
    64381 ctgatgccac cgtgatcggc cttttccggc gtctcatcac ccggcgcgcg ggaaaccgtg
    64441 cgccacggcg actcttccca cttgcggtca cggggctgac tctgtttgtc atcgcgggcg
    64501 gggcgacggc caccgtcagc acgagatttt cctggacgcc cgcccccttt cccggtacgc
    64561 gggttgtggg tacgtttatc agaatcatca tcactgcgga catacatcac tttgaccttg
    64621 ccgcttttac ctttcatttc atcgttcatg cttttctcca ccagcgctgc gcgaagcgcg
    64681 cagattaccc gaagtccgcg cggttcgcca tgatttcgta ccaaagcctg cgactatcat
    64741 acctattgaa taaaacagat tgttgtctgg aacaatgtcc ccgataatat gtaacatatt
    64801 agaaacatac cggcgtcgtt gccgataagt ctccttactc atcccgaggt tagttatgaa
    64861 taccgtttgt acccattgtc aggccatcaa tcgcattccc gacgatcgga tcgaagatgc
    64921 ggcaaaatgc ggacgctgcg gtcacgactt gtttgacgga gaggtgatta atgcgaccgg
    64981 tgaaacgctc gacaaattgc tgaaagatga tctaccagtg gtgatcgact tctgggcacc
    65041 gtggtgcggc ccctgccgta atttcgcacc aatttttgaa gatgtcgcgc aagagcgtag
    65101 cggtaaagtg cgctttgtga aagtgaatac cgaagctgaa cgtgaattga gcagtcgctt
    65161 tggaattcgt agtataccga cgatcatgat tttcaaaaac ggtcaggttg tcgacatgct
    65221 taatggcgca gtaccgaaag cgccgttcga tagctggctg aacgaatctc tttaatctta
    65281 ccggggcgca tcttgtgccc cgttttctcc tctgcgacaa tggcgttttt tcgacgctct
    65341 cttatgaccg aaaacgctgt tctccagtta cgcgccgagc gtattgcgcg cgcaacacgt
    65401 ccttttcttg cccgcggtaa tcgcgttcgt cgctgccaac gctgtctatt accagagaaa
    65461 ttatgtctct gttcaacgat cacgccagcg gaagcaaaaa gccgcttttg tttgttgatg
    65521 tttgacaccg agccaatgaa gcccagtaat accgggcgtc tgattgctga tattttgcct
    65581 gataccgttg catttcaatg gtcgcgtacc gaaccctcgc aaaatttgct ggatctggta
    65641 caaaacccgg actatcagcc aatggtggtc tttcccgcct cgtatgctga tgagcaacgg
    65701 gaagtgatct tcacaccacc tgccggtaag ccaccgctgt ttatcatgct cgatggtacc
    65761 tggccggaag ctcgcaagat gtttcgtaaa agtccgtatc tggataatct tcccgtcatt
    65821 tccgtcgatc tttcccggct ttctgcctat cgcctgcgtg aagcccaggc tgaaggccaa
    65881 tattgtactg ccgaagtagc catcgcactg ttagatatgg ccggcgatac cggggcggca
    65941 gcagggttag gcgagcattt tacccgcttt aaaacacgct atctggcagg aaaaacgcaa
    66001 catctgggta gcatcacagc agaacagtta gaaagcgttt aaaatcattc ggtcacttct
    66061 gcgggagacc ggtatgagtc agcgaggact ggaagcacta ctgcgaccaa aatcgatagc
    66121 ggtaattggc gcgtcgatga aacccaatcg cgcaggttac ctgatgatgc gtaacctgct
    66181 ggcgggaggc tttaacggac cggtactccc ggtgacgcca gcctggaaag cggtgttggg
    66241 tgtgttggcc tggccggata ttgccagctt gccctttaca cccgaccttg cggttttatg
    66301 taccaatgcc agccgtaatc ttgctcttct ggaagagctc ggcgagaaag gctgtaaaac
    66361 ctgcattatt ctttccgccc cggcatcgca acacgaagat ctccgcgcct gcgccctgcg
    66421 ccataacatg cgcctgcttg gaccaaacag tctgggttta ctggctccct ggcaaggtct
    66481 gaatgccagc ttttcgcctg tgccgattaa acgcggcaag ctggcgttta tttcgcaatc
    66541 ggctgccgtc tccaacacca tcctcgactg ggcgcaacag cgtgagatgg gcttttccta
    66601 ctttattgcg ctcggcgaca gcctggatat cgacgttgat gaattgcttg actatctggc
    66661 acgcgacagt aaaaccagcg ccatcctgct ctatctcgaa cagttaagcg acgcgcgacg
    66721 ctttgtttcg gcggcccgta gtgcctcgcg taataaaccg attctggtga ttaaaagcgg
    66781 acgtagcccg gcggcacagc gactgctcaa cacgacggca ggaatggacc cggcatggga
    66841 tgcggctatt cagcgtgccg gtttgttgcg ggtacaggac acccacgaac tgttttcggc
    66901 ggtagaaacc cttagccata tgcgcccgct gcgtggcgac cggttgatga ttatcagcaa
    66961 cggtgctgcg cctgccgcgc tggcgctgga tgccttatgg tcacgcaatg gcaagctggc
    67021 aacactaagc gaagaaacct gccagaaact gcgcgatgca ctgccaggac atgtggcaat
    67081 ctctaacccg ctcgatctgc gcgatgacgc cagcagcgag cattatgtca aaacgctgga
    67141 cattctgctc catagccagg attttgatgc gctgatggtt attcattcgc ccagcgccgc
    67201 tgctcccgca acagaaagtg cgcaagcatt aattgaagca gtaaagcatc atccccgcag
    67261 caagtatgtt tctctgctga cgaactggtg cggcgagcac tcctcgcaag aggcacgacg
    67321 tttattcagc gaagccgggc tgccgaccta tcgtaccccg gaaggaacca tcactgcttt
    67381 tatgcatatg gtggagtacc ggcgtaatca gaagcaacta cgcgaaacgc cggcgttgcc
    67441 cagcaatctg acttccaata ccgcaaaagc gcatcttctg ttgcaacagg cgattgccga
    67501 aggggctacg tcgctcgata cccatgaagt tcagcccatc ctgcaagcgt atggcatgaa
    67561 cacgctccct acctggattg ccagtgatag cacggaagcg gtgcatattg ccgaacagat
    67621 tggttatccg gtggcgctga aattgcgttc gccggatatt ccacataaat cggaagttca
    67681 gggcgtcatg ctttacttgc gcacagccaa tgaagtccag caagccgcga acgctatttt
    67741 cgatcgcgta aaaatgacct ggccgcaggc gcgggtccac ggcctgttgg tgcaaagtat
    67801 ggctaaccgt gctggcgctc aggagttgcg ggttgtggtt gagcacgatc cggtttttgg
    67861 gccgttgatc atgctgggtg aaggcggtgt ggagtggcgt cctgaagatc aagccgtcgt
    67921 cgcactgccg ccgctgaata tgaacctggc ccgctatctg gttattcagg ggatcaaaag
    67981 taaaaagatt cgtgcgcgca gtgcgctacg cccattggat gttgcaggct tgagccagct
    68041 tctggtgcag gtttccaact tgattgtcga ttgcccggaa attcagcgtc tggatattca
    68101 tcctttgctg gcttctggca gtgaatttac cgcgctggat gtcacgctgg atatcgcgcc
    68161 gtttgaaggc gataacgaga gtcggctggc agtgcgccct tatccgcatc agctggaaga
    68221 atgggtagaa ttgaaaaacg gtgaacgctg cttgttccgc ccgattttgc cagaagatga
    68281 gccacaactt cagcaattca tttcgcgagt caccaaagaa gatctttatt accgctactt
    68341 tagcgagatc aacgaattta cccatgaaga tttagccaac atgacgcaga tcgactacga
    68401 tcgggaaatg gcgtttgtag cggtacgacg tattgatcaa acggaagaga tcctcggcgt
    68461 cacgcgtgcg atctccgacc ctgataacat cgatgccgaa tttgccgtgc tggttcgctc
    68521 ggatctcaaa gggttaggct taggtcgacg cttaatggaa aagttgatta cctatacgcg
    68581 agatcacgga ctacaacgtc tgaatggtat tacgatgcca aataatcgtg gcatggtggc
    68641 gctggcccgc aagctcgggt ttaacgttga tatccagctc gaagagggga tcgttgggct
    68701 tacgctaaat cttgcccagc gcgaggaatc atgagtaagg tactggaaat gttgaccact
    68761 taatcgggac tggtgttatt attgcccgct tatgtcgtct gcattgcaca gaggaccctt
    68821 caatgaacag agaagaaatg cactgtgatg ttgtcaaaat ttaagcgtaa taaacatcaa
    68881 caacaccttg cccaactacc caagatttct caatcagttg atgatgtcga tttcttttac
    68941 gctcccgccg atttccggga gacgctgctg gaaaaaatag ccagcgcgaa gcagcgcatt
    69001 tgcattgtcg ccctgtatct cgaacaggat gacggtggca aaggcattct gaacgcgttg
    69061 tatgaagcta aaaggcagcg tccggaactg gatgtgcagg tgctggtcga ctggcatcgt
    69121 gcacaacgtg gacgcattgg cgctgcggca tctaacacta acgctgactg gtactgccgc
    69181 atggcgcagg aaaatccggg cgtagatgtt ccggtttatg gcgttccaat caatacccgt
    69241 gaagcccttg gtgttctgca ctttaaaggc tttatcatcg acgatagcgt actttatagc
    69301 ggtgccagcc tgaacgatgt ttacctgcat cagcacgata aatatcgcta cgaccgttat
    69361 catcttatcc gtaaccgtaa gatgtcagac attatgtttg aatgggttac acagaatatt
    69421 atgaatggcc gcggcgttaa tcgtctggat gatgttaatc ggccaaaaag cccggaaatc
    69481 aagaatgata ttcgtctgtt ccgccaggag ctgcgtgatg ccgcttatca tttccagggc
    69541 gatgccgaca acgatcagct ttccgtaacg ccgctagtgg ggctggggaa atcgagtctg
    69601 ttgaacaaga ccattttcca tcttatgcct tgtgccgagc agaaactaac catctgtacg
    69661 ccatacttca acctgccagc aatccttgtg cgcaatatta tccagttgct gcgcgaaggg
    69721 aaaaaggtcg aaattattgt tggtgataaa accgcgaacg acttctacat tccggaagat
    69781 gaacctttca agataattgg cgcattgcct tatctctatg agatcaatct gcgtcgtttc
    69841 ctgagccgtt tgcagtatta cgtcaatact gaccagctag tggttcggtt atggaaagat
    69901 gacgacaaca cctatcacct aaaagggatg tgggttgatg ataagtggat gttgatcacc
    69961 ggtaataacc tgaacccgcg cgcctggcgt ctggatctgg aaaacgccat tttgatccac
    70021 gatccacaac tggagcttgc gccgcagcga gagaaagaac tggagctgat ccgcgagcat
    70081 accaccatcg ttaagcacta tcgcgatctg caaagtattg ccgattatcc ggtgaaggtt
    70141 cgtaaactca tccgccgttt gcgccgtatc cgcatcgacc gattaattag ccgcatcctg
    70201 taatcacaac cccgtcctgt acggggtttg ttttttggag gccacgtttt gcgtattctt
    70261 tttgtctgtt cactattgtt actttctgga tgcagccata tggctaacga tagctggagc
    70321 gggcaggata aagctcaaca ctttatcgcc tcggcgatgc tttccgccgc cggaaatgaa
    70381 tattcacagc atcaggggat gagccgggat cgcagtgcca tgtttggatt gatgttctct
    70441 gtcagtttgg gggcgtcaaa agagctttgg gatagccgcc ccgaagggag cggctggagc
    70501 tggaaggatt tggcctggga tgtcgccggt gcaagcaccg gctataccgt ctggcaactg
    70561 acccgtcact aaagacgcat ccccttccct ttgcgatgta gcatcaaaga aaccagaaac
    70621 gccaccacgg ccatcaaggt cacataccag aagaaggctg tttccattcc tattgatttc
    70681 agcgacaacg ctacgtactc cgccgaacca ccaaatatag cattagcgac cgcatatgac
    70741 agaccaacgc ctaatgcgcg aacctgtgcc gggaacatct cagccttcag tattccactg
    70801 attgatgtat aaaaactcac tatcagcagg gcacacatca ccagaccaaa agcggcataa
    70861 ggcgaggaaa cgttttgcaa tgctgagaga ataggaacgg taaaaatcgc tgccagcgaa
    70921 ccgaaacata acattgaggt acggcgacca atcttatccg acagcgcgcc aatgagtggt
    70981 tgaataagca tgaatacaaa caatgcggca gtcataatgc cactcgccac gttggcatgc
    71041 attcccgcag tatttaccag atacttctgc atataagtgg tgaaggtata gaaacaaagg
    71101 gagcccgcag cggtaaaacc gagaaccatg atgaatgcac ggcgattgcg ccataatcct
    71161 ttcagagatc cagcttcttt taaagcgcgc gtttcttgtt gcgaagtttc atctaactga
    71221 cgacgtaacc acaacgccac aacagctaac acagctccta acgcgaaagg aatacgccat
    71281 ccccactctc tgagtgcagc gtcttccatg gtgtgttgta aaaccacgac aaccagtagg
    71341 gctagcagtt gtccgccgat caacgtcaca tactgaaatg atgcgtaaaa acctttgcgc
    71401 ccttcaacgg caacttcact catataggtg gcgctggtgc catattctcc gccaacggat
    71461 aatccctgaa ataaacgcgc gagaagcaat aatgccggag cccacgtacc gattgtttca
    71521 taaccaggca aacaggcgat taccagtgag ccgaaacaca tcatacacac cgatagcagc
    71581 atcgattttt tgcgaccatg tttatcggct atgcggccaa atagccaacc gcctattggg
    71641 cgcatcagga atcccgcagc aaaaacacct gctgtttgta gtagttgagt cgtcgtgttc
    71701 ccggaaggga agaagatgtg ggcaaagtag agtgaacaga acgagtagac atagaaatcg
    71761 aaccactcga ccagattacc tgaagaggcc cccacaatcg cccaaatgcg gcgacgtgta
    71821 tcactacttg tcagtttgct gtctgccgtt acagtacttt cagccatgcc attatgtctc
    71881 ctgccgtaat ccgatgcttt tgtcggtcgc ttttgtttat ttcttttggt aaagaaaatg
    71941 ttatacattt gttgtgtatc attatgcaac cttaaccatg aatttagtta gcaggaaaat
    72001 ggttattgag gagcttaagg ataaatttct gatgaggaga acacgtatgg aggtgggcaa
    72061 gttggggaag ccgtattcgc tgctgaatct ggcatatgtg ggagtataag acgcgcagcg
    72121 tcgcatcagg catttttttc accaacgcaa aaaggccatc ctgacggatg gcctttcgct
    72181 taatttgatg cctggcagtt ccctactctc gcatggggag accccacact accatcggcg
    72241 ctacggcgtt tcacttctga gttcggcatg gggtcaggtg ggaccaccgc gctaaggccg
    72301 ccaggcaaat tctgttttat cagaccgctt ctgcgttctg atttaatctg tatcaggccg
    72361 aaaatcttct ctcatccgcc aaaaca
//
LOCUS       DAPGRN010000010        67922 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPGRN010000010 DAPGRN010000000
VERSION     DAPGRN010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.010"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            223..1623
                     /gene="sthA"
                     /locus_tag="RHW33_000733"
     CDS             223..1623
                     /gene="sthA"
                     /locus_tag="RHW33_000733"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV1644277.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(1606..2523)
                     /gene="oxyR"
                     /locus_tag="RHW33_000734"
     CDS             complement(1606..2523)
                     /gene="oxyR"
                     /locus_tag="RHW33_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV1644278.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2790..4163)
                     /gene="argH"
                     /locus_tag="RHW33_000735"
     CDS             complement(2790..4163)
                     /gene="argH"
                     /locus_tag="RHW33_000735"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV1644279.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEELPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FARARLG"
     gene            complement(4224..5000)
                     /gene="argB"
                     /locus_tag="RHW33_000736"
     CDS             complement(4224..5000)
                     /gene="argB"
                     /locus_tag="RHW33_000736"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV1644280.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPIGTRILA"
     gene            complement(5008..6012)
                     /gene="argC"
                     /locus_tag="RHW33_000737"
     CDS             complement(5008..6012)
                     /gene="argC"
                     /locus_tag="RHW33_000737"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV1644281.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWSGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINAISGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQAQVAQALQQAYAHQPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAQTQSLI
                     "
     gene            6166..7317
                     /gene="argE"
                     /locus_tag="RHW33_000738"
     CDS             6166..7317
                     /gene="argE"
                     /locus_tag="RHW33_000738"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV1644282.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     NLGFNVEVQPVPGTRNKFNMLASTGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            7792..10443
                     /gene="ppc"
                     /locus_tag="RHW33_000739"
     CDS             7792..10443
                     /gene="ppc"
                     /locus_tag="RHW33_000739"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV1644283.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKTLWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            10625..12358
                     /locus_tag="RHW33_000740"
     CDS             10625..12358
                     /locus_tag="RHW33_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV1644284.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            12573..13424
                     /locus_tag="RHW33_000741"
     CDS             12573..13424
                     /locus_tag="RHW33_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1644285.1"
                     /translation="MYHDVSYLLSRLINGPLYLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(13411..13752)
                     /locus_tag="RHW33_000742"
     CDS             complement(13411..13752)
                     /locus_tag="RHW33_000742"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV1644286.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCLLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(13754..14632)
                     /locus_tag="RHW33_000743"
     CDS             complement(13754..14632)
                     /locus_tag="RHW33_000743"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV1644287.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIDTAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVMENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(14598..16895)
                     /locus_tag="RHW33_000744"
     CDS             complement(14598..16895)
                     /locus_tag="RHW33_000744"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV1644288.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAAGCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(16946..17266)
                     /locus_tag="RHW33_000745"
     CDS             complement(16946..17266)
                     /locus_tag="RHW33_000745"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV1644289.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(17281..18360)
                     /locus_tag="RHW33_000746"
     CDS             complement(17281..18360)
                     /locus_tag="RHW33_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV1644290.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLTVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18669..21170
                     /gene="ptsP"
                     /locus_tag="RHW33_000747"
     CDS             18669..21170
                     /gene="ptsP"
                     /locus_tag="RHW33_000747"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1644291.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGHSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWARESVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            21182..21844
                     /gene="fsa"
                     /locus_tag="RHW33_000748"
     CDS             21182..21844
                     /gene="fsa"
                     /locus_tag="RHW33_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV1644292.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            21855..22958
                     /gene="gldA"
                     /locus_tag="RHW33_000749"
     CDS             21855..22958
                     /gene="gldA"
                     /locus_tag="RHW33_000749"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV1644293.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            23232..23849
                     /locus_tag="RHW33_000750"
     CDS             23232..23849
                     /locus_tag="RHW33_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV1644294.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     DYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(23876..24781)
                     /gene="yijE"
                     /locus_tag="RHW33_000751"
     CDS             complement(23876..24781)
                     /gene="yijE"
                     /locus_tag="RHW33_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV1644295.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(24875..27055)
                     /gene="katG"
                     /locus_tag="RHW33_000752"
     CDS             complement(24875..27055)
                     /gene="katG"
                     /locus_tag="RHW33_000752"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV1644296.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(27384..28274)
                     /gene="metF"
                     /locus_tag="RHW33_000753"
     CDS             complement(27384..28274)
                     /gene="metF"
                     /locus_tag="RHW33_000753"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV1644297.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            28350..28595
                     /locus_tag="RHW33_000754"
     CDS             28350..28595
                     /locus_tag="RHW33_000754"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644298.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     LTNHVLIASCRIRRKRFIRPNPMIIQYISLETIDPKT"
     gene            complement(28623..31055)
                     /gene="metL"
                     /locus_tag="RHW33_000755"
     CDS             complement(28623..31055)
                     /gene="metL"
                     /locus_tag="RHW33_000755"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV1644299.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSSLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(31058..32218)
                     /gene="metB"
                     /locus_tag="RHW33_000756"
     CDS             complement(31058..32218)
                     /gene="metB"
                     /locus_tag="RHW33_000756"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV1644300.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            32495..32812
                     /gene="metJ"
                     /locus_tag="RHW33_000757"
     CDS             32495..32812
                     /gene="metJ"
                     /locus_tag="RHW33_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV1644301.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            32996..33604
                     /locus_tag="RHW33_000758"
     CDS             32996..33604
                     /locus_tag="RHW33_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV1644302.1"
                     /translation="MKYRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(33665..33877)
                     /gene="rpmE"
                     /locus_tag="RHW33_000759"
     CDS             complement(33665..33877)
                     /gene="rpmE"
                     /locus_tag="RHW33_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV1644303.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            34080..36278
                     /gene="priA"
                     /locus_tag="RHW33_000760"
     CDS             34080..36278
                     /gene="priA"
                     /locus_tag="RHW33_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV1644304.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPVFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIVLGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            36434..37459
                     /gene="cytR"
                     /locus_tag="RHW33_000761"
     CDS             36434..37459
                     /gene="cytR"
                     /locus_tag="RHW33_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV1644305.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            37551..38510
                     /gene="ftsN"
                     /locus_tag="RHW33_000762"
     CDS             37551..38510
                     /gene="ftsN"
                     /locus_tag="RHW33_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV1644306.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            38603..39133
                     /gene="hslV"
                     /locus_tag="RHW33_000763"
     CDS             38603..39133
                     /gene="hslV"
                     /locus_tag="RHW33_000763"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV1644307.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            39143..40474
                     /gene="hslU"
                     /locus_tag="RHW33_000764"
     CDS             39143..40474
                     /gene="hslU"
                     /locus_tag="RHW33_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV1644308.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            40541..41467
                     /gene="menA"
                     /locus_tag="RHW33_000765"
     CDS             40541..41467
                     /gene="menA"
                     /locus_tag="RHW33_000765"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV1644309.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVVGIFLSQWAA"
     gene            41560..42045
                     /gene="rraA"
                     /locus_tag="RHW33_000766"
     CDS             41560..42045
                     /gene="rraA"
                     /locus_tag="RHW33_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV1644310.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            42105..42779
                     /locus_tag="RHW33_000767"
     CDS             42105..42779
                     /locus_tag="RHW33_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644311.1"
                     /translation="MQNIILLFIYLLLIAVNHFRYKFLLSGNAGEMILYFANVFFNPL
                     ALSFIIATIICITIKKRSSRSFIRGTCWLMGLFLIYSSYTVWERHSIWDYTFPEPGIT
                     VTVPSKQWVANIVKQGPTLVTRDAHAILAIVAFSQNELGVHSIEELTATQNGTAEMHV
                     CDVQGFACAYQEGVQTMSDGKVRHAIFMTLLDNTNLIQLVAAIDPDYLDEYQEEVMKM
                     MLSARQ"
     gene            42902..43528
                     /locus_tag="RHW33_000768"
     CDS             42902..43528
                     /locus_tag="RHW33_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644312.1"
                     /translation="MVGEYIGSVLLGPLLLPVLVSGILCTFTKKTRNFASFTRGCCWV
                     LGVLLLSNVGNTFRLFTPWHYTFEKAAISVTVPNRHWNTVSISTDKTIDIRSEDNSVF
                     ISAFRLPAGRSADDSLEELKKMQRDNLKDQYNEETFQFHDCNAKHFTCKYQDVLINFD
                     GQQKRTISVYLEDTPRAVGIIALMEPDTADKYRQQAMEIMLSAKNTVK"
     gene            complement(43567..43812)
                     /gene="zapB"
                     /locus_tag="RHW33_000769"
     CDS             complement(43567..43812)
                     /gene="zapB"
                     /locus_tag="RHW33_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV1644313.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            44238..45083
                     /gene="glpF"
                     /locus_tag="RHW33_000770"
     CDS             44238..45083
                     /gene="glpF"
                     /locus_tag="RHW33_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV1644314.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            45106..46614
                     /gene="glpK"
                     /locus_tag="RHW33_000771"
     CDS             45106..46614
                     /gene="glpK"
                     /locus_tag="RHW33_000771"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV1644315.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            46844..47854
                     /gene="glpX"
                     /locus_tag="RHW33_000772"
     CDS             46844..47854
                     /gene="glpX"
                     /locus_tag="RHW33_000772"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV1644316.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            47951..48697
                     /gene="fpr"
                     /locus_tag="RHW33_000773"
     CDS             47951..48697
                     /gene="fpr"
                     /locus_tag="RHW33_000773"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV1644317.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETTAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(48702..49130)
                     /gene="uspD"
                     /locus_tag="RHW33_000774"
     CDS             complement(48702..49130)
                     /gene="uspD"
                     /locus_tag="RHW33_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV1644318.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(49157..49456)
                     /locus_tag="RHW33_000775"
     CDS             complement(49157..49456)
                     /locus_tag="RHW33_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV1644319.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(49668..50108)
                     /locus_tag="RHW33_000776"
     CDS             complement(49668..50108)
                     /locus_tag="RHW33_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV1644320.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            50209..50808
                     /locus_tag="RHW33_000777"
     CDS             50209..50808
                     /locus_tag="RHW33_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV1644321.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            50916..51683
                     /gene="tpiA"
                     /locus_tag="RHW33_000778"
     CDS             50916..51683
                     /gene="tpiA"
                     /locus_tag="RHW33_000778"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV1644322.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(51738..52493)
                     /gene="cdh"
                     /locus_tag="RHW33_000779"
     CDS             complement(51738..52493)
                     /gene="cdh"
                     /locus_tag="RHW33_000779"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV1644323.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(52600..53589)
                     /gene="sbp"
                     /locus_tag="RHW33_000780"
     CDS             complement(52600..53589)
                     /gene="sbp"
                     /locus_tag="RHW33_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV1644324.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGMFDQISKR"
     gene            complement(53908..54870)
                     /gene="pfkA"
                     /locus_tag="RHW33_000781"
     CDS             complement(53908..54870)
                     /gene="pfkA"
                     /locus_tag="RHW33_000781"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV1644325.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(55051..55953)
                     /gene="fieF"
                     /locus_tag="RHW33_000782"
     CDS             complement(55051..55953)
                     /gene="fieF"
                     /locus_tag="RHW33_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV1644326.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(56102..56602)
                     /gene="cpxP"
                     /locus_tag="RHW33_000783"
     CDS             complement(56102..56602)
                     /gene="cpxP"
                     /locus_tag="RHW33_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV1644327.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            56752..57450
                     /gene="cpxR"
                     /locus_tag="RHW33_000784"
     CDS             56752..57450
                     /gene="cpxR"
                     /locus_tag="RHW33_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV1644328.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            57447..58820
                     /gene="cpxA"
                     /locus_tag="RHW33_000785"
     CDS             57447..58820
                     /gene="cpxA"
                     /locus_tag="RHW33_000785"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV1644329.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETLKANQLWGEVLDNAAFEAEQMGKSFSV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(58926..59600)
                     /gene="yiiM"
                     /locus_tag="RHW33_000786"
     CDS             complement(58926..59600)
                     /gene="yiiM"
                     /locus_tag="RHW33_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV1644330.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(59749..60732)
                     /gene="kdgT"
                     /locus_tag="RHW33_000787"
     CDS             complement(59749..60732)
                     /gene="kdgT"
                     /locus_tag="RHW33_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV1644331.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(60992..61612)
                     /gene="sodA"
                     /locus_tag="RHW33_000788"
     CDS             complement(60992..61612)
                     /gene="sodA"
                     /locus_tag="RHW33_000788"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV1644332.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            61897..62931
                     /gene="rhaT"
                     /locus_tag="RHW33_000789"
     CDS             61897..62931
                     /gene="rhaT"
                     /locus_tag="RHW33_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HDV1644333.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(62928..63794)
                     /gene="rhaR"
                     /locus_tag="RHW33_000790"
     CDS             complement(62928..63794)
                     /gene="rhaR"
                     /locus_tag="RHW33_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HDV1644334.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSMGMAQARQIIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(63850..64686)
                     /gene="rhaS"
                     /locus_tag="RHW33_000791"
     CDS             complement(63850..64686)
                     /gene="rhaS"
                     /locus_tag="RHW33_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HDV1644335.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            64974..66443
                     /gene="rhaB"
                     /locus_tag="RHW33_000792"
     CDS             64974..66443
                     /gene="rhaB"
                     /locus_tag="RHW33_000792"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV1644336.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPETMCSEIQAACREMAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            66440..67699
                     /gene="rhaA"
                     /locus_tag="RHW33_000793"
     CDS             66440..67699
                     /gene="rhaA"
                     /locus_tag="RHW33_000793"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV1644337.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSRRG"
BASE COUNT        16578 a        18191 c        17376 g        15777 t
ORIGIN      
        1 aagatttcat aaagacaata acaaaaaagc acgcttcggc aagataaaac tatcagccat
       61 aataaaaagc gattaatagt gaataaatgg gttaagaagg aggcagacaa agcaaaggcc
      121 gctcacgcta tagccagatt aatgacgggg atcaattggc ttacccgcga taaaatgtta
      181 ccattctgtt gcttttatgt ataagaacag gtaagcccta ccatgccaca ttcctacgat
      241 tacgatgcca tagtaatagg ttccggcccc ggcggcgaag gcgctgcaat gggcctggtt
      301 aagcaaggtg cgcgcgtcgc agttatcgag cgttatcaaa atgttggcgg cggttgcacc
      361 cactggggca ccatcccgtc gaaagctctc cgtcacgccg tcagccgcat tatagaattc
      421 aatcaaaacc cactatacag cgaccattcc cgactgctcc gctcttcttt tgccgatatc
      481 cttaaccatg ccgataacgt gattaatcaa caaacgcgca tgcgtcaggg attttacgaa
      541 cgtaatcact gtgaaatatt gcaggggaac gctcgctttg ttgacgagca tacgttggcg
      601 ctggattgcc cggacggcag cgttgaaaca ctaaccgctg aaaaatttgt tattgcctgc
      661 ggctctcgtc catatcatcc aacagatgtt gatttcaccc atccacgcat ttacgacagc
      721 gactcaattc tcagcatgca ccacgaaccg cgccatgtac ttatctatgg tgctggagtg
      781 atcggctgtg aatatgcgtc gatcttccgc ggtatggatg taaaagtgga tctgatcaac
      841 acccgcgatc gcctgctggc atttctcgat caagagatgt cagattctct ctcctatcac
      901 ttctggaaca gtggcgtagt gattcgtcac aacgaagagt acgagaagat cgaaggctgt
      961 gacgacggtg tgatcatgca tctgaagtcg ggtaaaaaac tgaaagctga ctgcctgctc
     1021 tatgccaacg gtcgtaccgg taataccgat tcgctggcgt tacagaacat tgggctggaa
     1081 actgacagtc gcggacagct gaaggtcaac agcatgtatc agaccgcaca gccgcacgtt
     1141 tacgcggtgg gcgacgtgat tggttatccg agcctggcat cagcggccta tgaccagggg
     1201 cgcattgccg cgcaggcgct ggtaaaaggc gaagccaccg cgcatctgat tgaagatatc
     1261 cctaccggca tttacaccat cccggaaatc agctctgtgg gcaaaaccga acagcagctg
     1321 accgcgatga aagtgccata tgaagtgggc cgcgcccagt tcaaacatct ggcacgtgca
     1381 caaatcgtcg gcatgaacgt gggcacgctg aaaattttgt tccatcggga aacaaaagag
     1441 attctgggca ttcactgctt tggcgagcgc gctgccgaga ttattcacat cggtcaggcg
     1501 attatggaac agaaaggtgg cggcaacact attgagtact tcgtcaacac cacctttaac
     1561 tacccgacga tggcggaagc ctatcgggta gctgcgttaa acggtttaaa ccgcctgttt
     1621 taacacttta tcgaaatggc catccattct tgcgcggatg gcctctgcca gctgctcata
     1681 gcggctgcgc agcggtgagc caggacgata aaccaggcca atagtgcggc gtggttccgg
     1741 cttaatgcac ggcagataaa caaccccatc gcgtttgcgc tccggcggca cagccagcgc
     1801 aggcagtaaa gtgatcccgc tacctgccgc caccatgttg cgcagagttt ccaggctggt
     1861 cgcgcggaag tgtgtatctt catccgcccc ggcttcaaaa cagaaaccca ttgcctgatc
     1921 gcgcaaacag tgaccatctt ccagcatcag cagtttttcc ccagccagat cggccatcgg
     1981 tacgcattcg cggttcgccc acgggtggtc ttcatagata gccagcaaca ttggctcatc
     2041 aaacaacggc acttcaatga atgcttcgct ctctttcacc agcgcgagga tcacgcaatc
     2101 gagtttgccg ctgtccagtt gcgccagtaa ctggtgggtc tgtgcttcat gcagatacat
     2161 ttccagcttt ggaaaggtct ggtgcagcat cgggataata tgcggtagca ggtacggtcc
     2221 aaccgtggga atcaaaccaa tatgcagcgg tccggacatc gtctcgccct gctggcttgc
     2281 catctcttta aggactttca cctcacgcag cacggtacgc gcctgatcca ccagcagcat
     2341 tcccgcctgg gtgaacaaca ctttacggct ggtccgctcc agcaacatca cgcccagctc
     2401 atcttccagc ttacgaattt gcccgctaag cgtcggctgg ctaacgtggc aggaatctgc
     2461 cgcacgccga aaatggcggt gttcagccaa tgccaccagg tactcaagat cacgaatatt
     2521 cattatccat cctccatcgc cacgatagtt catggcgata ggtagaatag caatgaacga
     2581 ttatccctat caagcattct gactgataat tgctcacaga aacggagcgg cacctctttt
     2641 aacccttgaa gtcactgccc gtttcgagag tttctcaact cgaataacta aagccaacgt
     2701 gaacttttgc ggatctccag gatccgcttt tttttgccat aaaaaagccc ggcgataagc
     2761 caggctcaaa tttgtacata taaatgttct taccccaacc gcgcccttgc aaaagcaatc
     2821 gcctgcgcca cctgctgcgg tgagacgccg ccttttgccg cacgcttgtc gaggcacgat
     2881 tgcagcgaca aaatcggata gacatcttcg ccaatcaccg ggctgaattt ctgcaactcg
     2941 tcgagcggca gttcttccag cggtttgccc tgacgaatgg cttccaccac cgcttcacca
     3001 acaatatggt gcgcctcgcg gaaaggtacg cctttcgcca ccaggtaatc cgccagttcg
     3061 gtggcgttgg cgtaaccctg ctgagccgct tcctggcaac gtggacgttt cacctggatg
     3121 ccgtccagca ccagcgccgc catatgcagg cagtccagcc aggtatcgag cgcgtcgaac
     3181 agaccttctt tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc
     3241 atcatcatgc cggttaacgc gccctgcacc cgaccgcatt taccgcgaat cagctccagc
     3301 gcatccgggt ttttcttctg cggcattaat gatgaaccgg aagtcacgcg gtcagaaagc
     3361 tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaaa acgcgaaaga
     3421 tgcaccatgc cgattgcggc agccgacagc agttccagca cgtggtcacg gtcagaaacg
     3481 ctgtcgagac tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg
     3541 tcgatttcat aggccgttcc cgccagcgcg ccacagccta acggactgac atccagacgc
     3601 ttaagcgcat cctgcaagcg gctttcatca cgcgccagca tctccacgta agccaggcac
     3661 cagtgtgcga acgtcaccgg ttgagcacgt tgcaggtggg tatagcccgg cataaccgcg
     3721 tcctgattat tctgcgccgt ctcgaccaga gcggattgca gctgacggtt agccgtcagc
     3781 aactcgccaa cggtatcttt gcaccacagt ttcaggtcag tcgctacctg atcattacga
     3841 ctacgcccgg tatgcagctt tttgcctaac tggcccactt tgtcgatcag tttgccttcc
     3901 acccagctat ggatatcttc ggcgtcgctt tcaaggattt gttgtggtct ggcgcgaaca
     3961 tcttccagca gcacgttcag cgcctcttcc agttgcgcct gttcttctgc ggttagcaca
     4021 ccgaccgtta ccagggcttt ggaccaggcc acagagccga caatatcctg ctccgccaga
     4081 cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta
     4141 aaacgcccgc cccaaagtgc cataactctg tttccttatt ttgaaattca atgccgaata
     4201 gcgatgcctc cggccaacaa aactcaagct aaaatccgcg taccaatagg cataccgtta
     4261 aacagtgccg gaagctgttc cgcatgacgc caggaggcga tatctacagg gcggcccagc
     4321 gtgcgcgcag catccagcgc cgcgttcact ttcactatca tgccgtcagt aataatgccc
     4381 tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg caatacgttg ccctttaccg
     4441 tcgagaatgc cgctgacgtc agagagcaaa atcagatccg cgcccagcgt tgccgccagc
     4501 gccgtcgccg cctggtcagc attgacgttc atcagttgcc cttcgtctgt tacgccaatg
     4561 gagctgacca ccggcagata accgttctcc agcaaggtgt tgataagctt aggcgaacct
     4621 ggctgcgcca gtccaacatg gcctaactct tcatcaagct gggtcacttt gacgctgtcg
     4681 ccgtcaccga gaaacaaacc tacggccgca atctgatgtt tcttcgccca tgccagcagg
     4741 gttttatttg ccgttcccgc cagtgctccg gtgataatgt ctatctgatc cgcaggcgtc
     4801 acccgcaggc cgtttttctt tttcaccggc agattcagcc ctttcatcag ctcatccacc
     4861 acgcaaccgc cgccgtggac aatcaccagc ggacgctgat gtgactcacg ataattcacc
     4921 agtgcgctaa acagacgttc cagcgcctct tcactatcca gcagtacgcc gcccagtttg
     4981 ataattaatg gattcatcat tgcaccctta aataagagac tgcgtttggg catagccgaa
     5041 acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt
     5101 cgccacaata atcaaatgct cgccctgaac ggcaaacccg atatcgcaaa atggcagccc
     5161 aacgacattt ttcagcgccg gaacgccttt gtcatacagc cgcaccagcg gttgatgcgc
     5221 atacgcctgt tgtaacgctt gcgcgacttg tgcctggctc acgcccggtt tcaggcggca
     5281 ggtaatggtt tccagaatgc cgcgtgggaa attacccaga tgcggggtga aaatcacgtc
     5341 agcaccaaga tgcgtggcga tctccggttg atggcgatgg gtaaagacac cgtatggttg
     5401 caggctaact tcgcaaaagc tgtttgaaat ggccgcttta cgccctgcac cactcacgcc
     5461 gctgatggcg ttaatcaccg gccactgatt gaggtcaaga agatcggcat caatcaacgg
     5521 tttcagcgcc agctgtgccg ccgtcggata acagcctggc accgcaatca aattcgcttc
     5581 ttttaattta ttaccgctcc actccgccag accgtaggct gcctgttcca gcagttccgg
     5641 gtattgatgg gtaaagccgt aatatttttc atagaaggtg gcgtcgttaa cacgaaacgc
     5701 gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac tgcggcgcta aatcgtggct
     5761 aacttcatgg gcggtggcga gaaacactac gtccacccct gggctaaact cgctgatatc
     5821 cgacatcggc tgcaacggca gatcaacgat gccttttagc tgcggatgca aatcggagat
     5881 taactttccc gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg
     5941 atggcgattt acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag
     6001 cgtattcaac atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac
     6061 attcacctta cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa
     6121 tactgcatga atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta
     6181 ccgccattta tcgagattta ccgtgctctg attgccacac cttcaataag cgccacagaa
     6241 gaggcactcg atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaaaat
     6301 ttaggcttca atgtggaagt gcagcctgtg ccaggaactc gcaacaaatt caatatgctg
     6361 gcaagtaccg gacagggggc tggcggcttg ttgctggcgg ggcataccga tacggtgcca
     6421 tttgatgacg gtcgctggac gcgcgatccg tttacactga cggagcatga cggcaagctt
     6481 tacggcttag gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc
     6541 gatgtcgacg tcacgaaact gaaaaaaccg ctctacattc tggcgactgc tgatgaagaa
     6601 accagtatgg ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc
     6661 atcattggcg aaccgacgtc actacaaccg gtacgcgcac ataaaggtca tatctctaac
     6721 gccatccgta ttcagggtca gtcggggcac tccagcgatc cagcacgcgg agttaacgct
     6781 atcgaactaa tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa
     6841 cgttatcact acgaagcgtt taccgtgcca taccctacgc tcaacctcgg gcatattcac
     6901 ggtggcgacg cgtctaaccg tatttgcgct tgctgtgagt tgcatatgga tattcgtccg
     6961 ctgcctggca tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccggtg
     7021 agcgaacgct ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat
     7081 gaatgcccgc caaatcatca actggttgaa gtggttgaga aattgctcgg agcaaaaacc
     7141 gaagtggtga attactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtt
     7201 ctggggcctg gctcaattaa tcaggctcat cagcctgatg aatatctgga aacacggttt
     7261 atcaagccca cccgcgaact gataacccag gtcattcacc atttttgctg gcattaaaac
     7321 gtaggccgaa taaggcgctt gcgccgcatc cggcactgtt gccaaactcc agtgccgcaa
     7381 taatgtcgga tgcgatgctt gcgcatctta tccgacctac agtgactcaa acgatgccca
     7441 accgtaggcc ggataaggcg ctcgcgccgc atccggcact gttgccaaac tccagtgccg
     7501 caataatgtc ggatgcgata cttgcgcatc ttatccgacc tacacctttg gtgttaattg
     7561 aggcgatttt ttaacattcc cataagttac gcttatttaa agcctcgtga atttaatgac
     7621 gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga cgtcatcgct
     7681 tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg cgccaatgcg
     7741 acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa tatgaacgaa
     7801 caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg agaaaccatc
     7861 aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa gttgtccaaa
     7921 tcttcacgcg ctggcaatga tgctaaccgc caggagttac tcaccacctt acaaaatttg
     7981 tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa cctggccaac
     8041 accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa cccggaagtg
     8101 atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga caccatcaaa
     8161 aaagcagtgg aatcgctgtc actggaactg gtcctcacgg ctcacccaac cgaaattacc
     8221 cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gcttaaaaca gctcgataac
     8281 aaagatatcg ctgactacga acacaaccag ctgatgcgtc gcctgcgcca gttgatcgcc
     8341 cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga tgaagccaaa
     8401 tggggctttg ccgtagtaga aaacagcctg tggcaaggcg taccgaatta cctgcgcgaa
     8461 ctgaacgaac aactggaaga gaacctcggc tacaaactgc ccgtcgaatt tgttccggta
     8521 cgttttacct cgtggatggg cggtgaccgc gacggcaacc caaacgtcac tgccgatatc
     8581 acccgccacg tcctgctgct cagccgttgg aaagccaccg atctgttcct gaaagatatt
     8641 caggtgctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct ggcgctggtt
     8701 ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg ttctcgcctg
     8761 atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc aaaaccagaa
     8821 ggcctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta ccagtcactt
     8881 caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct gcgccgcgtg
     8941 aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac gcgtcatacc
     9001 gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag ctggtcagag
     9061 gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct tctgccgcgc
     9121 aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt gattgccgaa
     9181 gcaccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc gtccgacgtg
     9241 ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcaatgcc ggttgctccg
     9301 ctgtttgaaa ctctcgatga cctgaacaac gccaacgatg tcatgactca gttgctgaat
     9361 atcgactggt atcgcggcct gattcagggc aaacagatgg tgatgattgg ctattccgac
     9421 tcagcaaaag atgcgggcgt gatggcagcg tcctgggcgc aatatcaggc acaggatgca
     9481 ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg tcgcggcggt
     9541 tccattggtc gcggcggcgc acctgctcat gcggcgctgc tgtcacaacc gccaggaagc
     9601 ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa atatggtctg
     9661 ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg cgattctgga agccaacctg
     9721 ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact atcagtcatc
     9781 tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc ttacttccgc
     9841 tccgctacgc cggaacagga actgggcaaa ctaccgttgg gttcacgtcc ggcgaaacgt
     9901 cgcccaaccg gcggcgtcga gtcattacgc gccattccgt ggatcttcgc ctggacgcaa
     9961 aaccgtctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa agtggtcgaa
    10021 gatggcaaac agagcgagct ggaggctatg tgccgcgatt ggccattctt ctcgacgcgt
    10081 ctcggcatgc tggagatggt cttcgccaaa gcagatctgt ggctggcgga atactatgac
    10141 caacgcctgg tagacaaaac actgtggccg ttaggtaaag agttacgcaa tctgcaagaa
    10201 gaagacatca aagtggtgct ggcgattgcc aacgattccc atctgatggc cgatctgccg
    10261 tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa cgtattgcaa
    10321 gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc ggatcctcgc
    10381 gtcgagcagg cgttaatggt cactattgcc gggattgcgg caggtatgcg taataccggc
    10441 taatcttcct ctcctgcaaa ccctcgtgct tttgcgcgag ggttttctga aatacttctg
    10501 ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt ctggatatcc
    10561 cttcagatat ccttaaggaa ttgtcggtac attcggcgat attttttcaa gacaggttct
    10621 tactatgcat tccacagaag tccaggctaa acctcttttt agctggaaag ccctgggttg
    10681 ggcactgctc tacttttggt ttttctctac tctgctacag gccattattt acatcagtgg
    10741 ttatagtggc accaacggca ttcgcgactc gctgttattc agctcgctgt ggttaattcc
    10801 ggtattcctc tttccgaagc ggatcaaaat tattgccgca gtgatcggcg tggtgctatg
    10861 ggcggcctct ctggcggcgc tgtgctacta cgtcatctac ggtcaggagt tctcgcagag
    10921 cgttctgttt gtgatgttcg aaaccaacac caacgaagcc agcgagtatt taagccagta
    10981 tttcagcctg aaaattgtgc ttatcgcgct ggcctatacg gcggtggcag ttctgctgtg
    11041 gacacgcctg cgcccggtct atattccaaa gccgtggcgt tatgttgtct cttttgccct
    11101 gctttatggc ttgattctgc atccgatcgc catgaatacg tttatcaaaa acaagccgtt
    11161 tgagaaaacg ttggataacc tggcctcgcg tatggagcct gccgcaccgt ggcaattcct
    11221 gaccggctat tatcagtatc gtcagcaact aaactcgcta acaaagctac tgaatgaaaa
    11281 taatgccttg ccgccgctgg ctaatttcaa agatgaatcg ggtaacgaac cgcgcacctt
    11341 agtgctggtg attggcgagt caacccagcg tggacgcatg agtctgtacg gttatccgcg
    11401 tgaaaccacg ccggagctgg atgcgctgca taaaaccgat ccgaatctaa ccgtgtttaa
    11461 taacgtagtt acgtctcgtc cgtacaccat tgaaatcctg caacaggcgc tgacctttgc
    11521 caatgaaaag aacccggatc tgtatctgac gcagccgtcg ctgatgaaca tgatgaaaca
    11581 ggcgggttat aaaaccttct ggatcaccaa ccagcagacg atgaccgccc gcaataccat
    11641 gctgacggta ttttcgcgcc agaccgacaa gcagtactac atgaaccagc aacgtacaca
    11701 gagtgcgcgt gaatacgaca ccaacgtgct gaagccgttc caggaagtgc tgaatgaccc
    11761 tgcgccgaag aaactgatca tcgttcatct gctgggtacg catatcaaat acaaataccg
    11821 ctacccggaa aatcagggca agtttgatgg caataccgat catgttccac cgggattaag
    11881 cgcagaagag ctggagtcat ataacgatta cgacaacgct aacctgtata acgatcatgt
    11941 ggttgccagc ctgattaaag actttaaagc ggcagacccg aacggattcc tggtttactt
    12001 ctctgaccac ggtgaagagg tttacgacac accgccgcac aaaactcagg ggcgtaacga
    12061 agacaacccg acgcgtcata tgtataccat tccgttcctg ctgtggacgt cggaaaaatg
    12121 gcaagcgact catccgcgtg atttctcaca ggatgttgat cgtaaataca gcctggcgga
    12181 actgatccac acctggtcag atttggcggg cttatcttac gacggttacg atccaacccg
    12241 ttcagtggtg aatccgcagt tcaaagaaac tacccgctgg attggtaacc cgtacaagaa
    12301 aaacgcgctg atcgattacg acactctgcc gtatggcgac caggtgggta atcagtaatt
    12361 atcagccagg cgctatttgc cggatgcgac gcttgacgcg tcttatccgg cctacagatc
    12421 gcggcggtag actggataag gcgtttacgc cacatccggc aatacaactg ccgcaatgtt
    12481 tacatcgaac tcccctcagg ccacactttt caggtggcct tttttaataa tgccagttac
    12541 tgttttcccg tctacatacc taagggaata acatgtatca cgacgtcagc tatctgctct
    12601 cccgcctgat caacggcccg ctgtacctgc gccagattta ctttgccagc agtaacggcc
    12661 cggtgcccga tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg
    12721 agtttgttga taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttcctg
    12781 ctggtggctg gaattttcca caatggcaag cccccgccac tacctttagc gtgctgtttg
    12841 gcaaacagca actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg
    12901 cgaagcaaca cgtcgcccga cgcggcccac gcataggttc ttttctgcta caaacgctca
    12961 atgaaatgca aatgcagccg caggagcagc aaacggcaag gcttatcgtc gccagcctgc
    13021 ttagccactg ccgcgatttg cttggcagcc aaatccagac cgcctcacgt agccaggcac
    13081 tctttgaagc tattcgtgat tatatcgacg aacgctatgc ctccgcgctt actcgcgaat
    13141 ctgttgcaca ggcattctat atttcgccaa attacctctc gcacttgttt caaaaaacgg
    13201 gggccattgg ttttaacgaa tacctgaatc acacgcgact ggaacatgca aaaacgttat
    13261 tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca
    13321 actacttctg ccgcctgttt cgtaaaaata ccgaacgctc gccgtcggaa tatcgccggc
    13381 agtaccacag ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg
    13441 cgcagaaagc actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaaagcgta
    13501 gatgctgcat tgcacgtagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc
    13561 cgtaatcaac agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt
    13621 tcccagcgcg ccctgagttt caatgctgac tccccacttc tctaacaggc acaacttttc
    13681 cagccgttcc gccgccatat aagtatgcgc cacgccactg acgcaggcgg ttactgccac
    13741 caggtatgcc attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc
    13801 gcatcgttgt cacatcggct gacgacggcg caggcacctc tttcatcgac catgttttcc
    13861 ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg taacagatgg atctgcctga
    13921 ttttcagcgg gatcagtaca tccagcgcct gctgcatatt ctcccggctg agcgtgaaac
    13981 cagggatcag cggtaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcagat
    14041 tctccatcac gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcatccatga
    14101 tttttaaatc gaacaacact tcatcgcaca atttcgccag cggtaacagc ttgctggctg
    14161 gcgcgtctcc ggcagtatca atggcgcatg acacacccca cagccgcagt cgctgtaaaa
    14221 aacgggtagc aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc
    14281 cggacgtgcg aaagaaaatg tcatctttca tcacttcccg ttccagcgcg tcaaggctga
    14341 tatcgcgacc aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt
    14401 tcgcacagtg cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact
    14461 ccggattagc acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa
    14521 tgccctcgcc gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcattgc
    14581 ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc
    14641 tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca
    14701 taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga
    14761 atatgctgca acttaagctg ggtaaacgcc cgtaagaagt cggccagttt gcgtaatccc
    14821 gcttcacctt ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc
    14881 agcgtgttat cgagcttact gactgacttc agtaccgctg ttggcccttg tgcgtcctgg
    14941 cccagcatgg gtgacaagcc gccgtctgcc agctgttctc cggcaaaacg accgtctggc
    15001 gtcgcgccaa ccaccgatcc caacgggacg tgagcagaaa cggtatacga tcccggcgtg
    15061 aagtaaccgc cgcgcgggtt ctggtatttt tccacttctt tgcagtagtg gcgcaacagt
    15121 tcggcgctaa tgttatccac ctcgtcaata tcgttaccgt atttctcaaa gcggttaatt
    15181 aagcgagcgc ggactttttc gccttctggc gttgcaaagt tggcttttaa taccgacagc
    15241 aattcgtcaa aacttaaacg ctgttgatca aaaaccatcc ctttcaacgc atggagagaa
    15301 tcgctcaggt tggcgatacc gatcccctgt acgccggaga aattataacg cgcgccgcca
    15361 tcggtaatat cgcggccttt ttccagacaa tcgctgataa acgatgagag cagcggtaca
    15421 ggtgcccagt cgcgatggcc gatatcacaa atattgctgc cctcaaccat cagggtgatg
    15481 tagtggctga tcttggcgcg gatctgctcc agtaaacctt cataagtcag cgcagcattg
    15541 ccttcatttt catgcaggca gatttccatc actttcagca aattaaacat cgcgatgtca
    15601 tgcaagccgt aggttctgcc gggaatagat aattccacac agcccactac ggaatagtcg
    15661 cgcgcatctt ccagcgaaac gccacggttg aggaacgctg gcaccaccac ttcatcgtta
    15721 aagatttgcg gaataccggt gccgaggcga atggtttcgg cggttttcat caggaacggc
    15781 gtgtcgatca aggcgttagt gcgcacgccg aggttcggtt gcggtaattg tacgctttga
    15841 taggcatcga ggcagaggaa cgaaagcacg ttcaccgcgc tacgtccgtt ctcggttaac
    15901 ccgccgagca gtgcggtata gccagtcggg aaaccggcga aataacgcgc gctgctggtg
    15961 gagcgcaaca gcacaatgtc gttgcatttc acccataaag attcgagcag ttctttcagg
    16021 aacgccggat cttcgccctg ggttaaagat gcctgataga acggcaacat atactggtcg
    16081 aagcgcccta aagacagcga actggcgttg gattcgtatt gcagaatgat gttcatgtac
    16141 cagaataact ggcacgcctg ccagaacgtc tgcggcttat gttcggcgtt atggcgggag
    16201 atctccgcaa tagtcagcag ttcttcgcga cgctgggcat cagtgcagcc tgccgccatc
    16261 gtttccgcca gttcggcgta acgcaaaatg tgtttctgcg aggcttcgag cagtaacagc
    16321 gctgcctgat aaaagtgatt ctccggctgt tgctgacaat gttgctgcat ctgcgctacc
    16381 agctcaccca gcccgtgatt cagcaggcgc gggtaatcaa taataatgtg cccctggcct
    16441 ttatccgtct ggttgatgct gaaaatctgc gtgctggtcg cggcttttac ttcatccgtc
    16501 atctgcccgt taatgaaatc tttcatcgaa cgtttttccc agtacgggaa caactcttcg
    16561 cggtagatac gtttatcttc ttcgctgata gcaaagcggt cctgcggacg cgtcgggaat
    16621 tgatccagct ctttcagcag ccagtaaggg tccatttccg gcgacataat tccggcgcgc
    16681 ggttttacgg tgcggttacc ggcaatcagt tcttcatcac gaatcgaaat ttcaacatgt
    16741 tcaaggatat acgctgttgc tttcgcccgg cgcaatatca ccggttcgcc ttcggtttgc
    16801 cgatggctgg cggtataaag cagcgcacgc tccagcgaga tttcacgggt attggcaaac
    16861 agtgcagttt tgaggcgaga gatacgattc gtcatgagga actcctgtgg gaaagcgaac
    16921 ccgcccttac gggcgggata agaacttaag cggtttgcgc caggtgagct tcaattttgc
    16981 tcataatggc gtcagcgcgt ttaaccgcat cgctgatgtt gacgcgcacg atggtcttgc
    17041 cagcaaaacg ctcttcaaac ttgatgccga tatctttggt cagaatcacc atatcggcgc
    17101 tcgccacgtc ttccgctgtc agttcatttt ccagaccaat tgacccctgg gtttctactt
    17161 tcacttccca gcctttcgct ttcgcggcac tttccagcgc ctctgccgcc atataagtat
    17221 gggcaacacc agatggacac gcggttactg caataatttt cgtcatggct cgttcctcaa
    17281 ttaattaatt tcaaaatcca aatccaggtc gtcttctttt tcatcagtcg aagacccatt
    17341 tttacgcgcc agacttttca gcacgttcac acaaacagcc gtcaccactg ctccgacagc
    17401 cactgctgcg atataaccca gcttgccttc aaccaccggc agcacaatca gaccacccca
    17461 gcctgcatag cactgcgcac cgaccagcgc cgcagttact gcaccacaaa ctgaaccaac
    17521 catgatggaa ggaattacac gcagcggatc ggcagcggcg aaaggaatcg ccccttcagt
    17581 aaccccaacg caccccatca ccagcgccgc tttaccagtt tcgcgctctt ctgcggagaa
    17641 atttttacga ccaatcagcg tcgccagccc catccccagc ggcgggatac aaatacccac
    17701 ggcagcaatg gccaccacgg tatatacacc ctgagcaacg caaatcagca tgaaggcata
    17761 ggccactttg ttaaccggac cgcccatatc gaacgccagc atcagaccca tgatcaccgt
    17821 cagcataaca atgctgccct gctgcatccc ctgaagccac tgagtcaggc tgttggtcaa
    17881 cgcccctaca ggctcgccca agccccacat catgatgcct gcggtaatca gtgtgccgac
    17941 gataggaatg atgaagatag gcatcaccga gcgcagaact ttatgcaccg gaattttctt
    18001 caggtaatgc accacgatgc cgccgataat cccggcgatc agtgcaccaa agaagcccgc
    18061 accaaagctg ttaccaaccc aggcaccgat agcgcacgga gccagcgcag aacgctctgc
    18121 aatggagtaa ccaatataag cggcgaggaa aggcaccatc agcgtcaaac ctgcaacgcc
    18181 gatatcaaac agttttttca aattcggatc ggctacggca tccggcactg cgcctttgcc
    18241 atacaacatg acggaaaccg ccagcaaaat accgcccgat accacgaagg gaatcatgtg
    18301 tgaaacgccc gtcattaaat gctgacgggt atttttcagg atctgcacca actcattcat
    18361 aagctgctcc cgggcttgcg aaagcccatg tccatatttt tgatggagga aacaataggc
    18421 aatcgcaggc ggagcaaaca gtggataaaa aggccattta ctggactttt gtaatattag
    18481 tacaaaaatg cgatccgcct cataacttgc gataatccgg aagtatgccg caaaacggcg
    18541 tcttgtgaga cgcgtcgcaa attgacggtt acattacatt tgtccagctt ttggcagatt
    18601 tgtcacatgg caggccgttg ccgtgcgctc ttatcctgta aacagaaaac cgtaccggga
    18661 gagaggcaat ggccctgatt gtggaattta tttgtgagct acctaacggc gtacatgcac
    18721 gtccggcaag ccacgttgaa acgctgtgta atactttttc atcacaaatt gagtggcata
    18781 acctacgcac tgaccgcaag ggcaacgcca aaagcgccct tgcgctgatt ggcaccgaca
    18841 cgctggcggg cgataactgc cagttactga tttccggggc cgacgaacag gaagcgcacc
    18901 agcgtttaag ccaatggctg cgcgatgaat tcccccactg cgatgcgccg ctggcggaag
    18961 ttaaatctga cgaactggaa ccactgccgg tttcactgac caatctgaat ccgcaaatta
    19021 tccgcgcccg caccgtgtgc agcggtagcg caggcggcat tctgacgccg atctcttctt
    19081 tagatctcaa tgcgctgggt aatcttcccg cagccaaaga cgttgacgcc gagcaatccg
    19141 cactggaaaa cggcctgacg ctggtactga aaaacattga gtttcgtctg ctggatagcg
    19201 acggtgccac cagcgcgatt ctggaagcgc accgatccct ggctggcgat acttccctgc
    19261 acgaacattt actggcaggc gtcagcgccg gattaagctg cgccgaagcg attgtcgcca
    19321 gtgcgaatca cttttgtgaa gagttttccc gttccagcag cagctacctg caagaacgtg
    19381 ccctggacgt acgcgatgtc tgcttccagc tgcttcagca aatctacggt gagcaacgct
    19441 tcccggcacc gggcaaactg acgcagcccg ccatttgtat ggctgatgaa ctaaccccca
    19501 gccagttcct cgaactggat aaaaatcacc tcaaaggtct gttactgaaa agtggcggca
    19561 ccacctcaca taccgtaatt cttgcccgtt cgttcaacat tccgacactg gttggtgtag
    19621 atattgatgc ccttactccg tggcagcatc aaacgattta tatcgacggc aacgccgggg
    19681 cgattgtggt tgagccaggg gaagccgtag ctcgttatta tcagcaagaa gcccgcgtac
    19741 aggacgccct gcgtgagcaa cagcgtgtct ggctgaccca acaagcccgt accgctgacg
    19801 gtatccgcat tgaaattgcc gctaacatcg ctcactccgt ggaagcgcag gccgcattcg
    19861 gcaatggtgc ggaaggcgtt ggtttgttcc gcactgaaat gctctatatg gatcgcacca
    19921 gtgcaccggg cgaaagcgag ctgtacaaca ttttttgtca ggcgctggag tctgccaacg
    19981 gacacagcat tattgtgcgc actatggaca ttggcggcga caaacccgtt gattatctga
    20041 acattccggc agaagctaac ccgttcctcg gttatcgcgc tgtgcgtatt tatgaagaat
    20101 acgcctcgct gttcaccaca caactacggt cgatcctgcg cgcctctgcg cacggcagcc
    20161 tgaaaatcat gatcccgatg atctcctcaa tggaagagat cttatgggtg aaagaaaaac
    20221 tggcggaagc caaacagcaa ctgcgtaacg aacacattcc gtttgatgag aagatccagc
    20281 tcggcatcat gctggaagtg ccgtcggtga tgttcatcat cgatcaatgc tgcgaagaga
    20341 ttgatttctt tagtattggt agtaatgacc tgacgcaata tctgctggca gtggatcgcg
    20401 ataacgctaa ggttactcgt cactacaaca gcctgaatcc ggcattcttg cgggcgctcg
    20461 attacgccgt gcaagcggtg catcgccagg gcaaatggat tggtctgtgc ggtgagctgg
    20521 gagcgaaagg ttccgtgctg ccgttgctgg tcggcttagg gctggatgaa ctcagcatga
    20581 gcgcaccatc aattccggcg gcgaaagcgc ggatggcgca acttgatagc cgtgagtgcc
    20641 gcaagttgct caaccaggca atggcctgcc gtacttcgct ggaagtggaa cacctgctgg
    20701 cgcaattccg catgacccaa caagacgcac cactggtcac cgccgagtgc atcacgctgg
    20761 aaagcgactg gcgcagcaaa gaagaagtac tcaaaggcat gaccgataac ctgctgctgg
    20821 cgggccgctg ccgctatccg cgtaaactgg aagccgactt gtgggcgcgc gagtccgttt
    20881 tctctaccgg tctgggcttt agttttgcca ttccccacag caaatcagaa cacattgagc
    20941 aatccaccat cagcgtagcg cgtctgcaag caccggtgcg ctggggcgat gatgaagcgc
    21001 aattcatcat tatgttaacc ctgaacaaac acgctgcggg cgatcagcac atgcgcattt
    21061 tctcgcgcct cgctcgccgc atcatgcacg aagaattccg taacgcgctg gttaacgccg
    21121 cctctgccga cgctatcgcc agcctgctgc aacatgaact ggaactgtaa aaggaaacat
    21181 catggaactg tatctggaca ccgctaacgt cgcagaagtc gaacgtctgg cacgcatatt
    21241 cccgattgcc ggggtgacaa ctaacccgag cattatcgct gccagcaagg agtccatctg
    21301 ggaagtgctg ccgcgtctgc aaaaagcgat tggtgatgag ggcattctgt ttgctcagac
    21361 tatgagtcgt gacgcgcagg ggatggtgga agaagcgaag cgtctgcgcg acgccattcc
    21421 gggcattgtg gtgaaaatcc cggtgacttc cgaaggtctg gcagcaatta aaatactgaa
    21481 aaaagagggt attactacac ttggcaccgc tgtatatagc gccgcacaag ggttattagc
    21541 cgcactggca ggggcaaaat acgttgctcc gtatgttaac cgcgtcgacg cccagggcgg
    21601 ggacggcatt cgtacggttc aggagctgca agcgctgtta gaaatgcacg caccagaaag
    21661 catggtgctg gcggccagct ttaaaacgcc gcgtcaggcg ctggactgtt tactggcagg
    21721 atgtgaatcc atcaccctgc ccttagatgt agcgcaacaa atgctcaaca cccctgcggt
    21781 agagtcagct atagagaagt tcgaacacga ctggaatgcc gcatttggca ctactcatct
    21841 ctaaaggagc aattatggac cgcattattc aatcaccggg taaatacatc cagggcgctg
    21901 atgtgattaa tcgtctgggc gaatacctga agccgctggc agaacgctgg ttagtggtag
    21961 gtgacaaatt tgttttaggt tttgctcaat ccactgtcga gaaaagcttt aaagatgctg
    22021 gactggtagt agaaattgcg ccgtttggcg gtgaatgttc gcaaaatgag atcgaccgcc
    22081 tgcgtggcat tgcggagact gcgcagtgtg gcgcaattct cggtatcggt ggcggaaaaa
    22141 ctctcgatac tgccaaagca ctggcacatt tcatgggtgt tccggtagcg atcgcaccga
    22201 ctatcgcctc taccgacgca ccgtgcagcg cattgtctgt tatctacacc gatgagggtg
    22261 agtttgaccg ctatctgctg ttgccaaata acccgaatat ggtcattgtc gacaccaaaa
    22321 tcgtcgctgg cgcacctgca cgtctgttag cggcgggtat cggcgatgcg ctggcaacct
    22381 ggtttgaagc gcgtgcctgc tctcgtagcg gcgcgaccac catggcgggc ggcaagtgca
    22441 cccaggctgc gctggcactg gctgaactgt gctacaacac cctgctggaa gaaggcgaaa
    22501 aagcgatgct tgctgccgaa cagcatgtag tgactccggc gctggagcgc gtgattgaag
    22561 cgaacaccta tttgagcggt gttggttttg aaagtggtgg tctggctgcg gcgcacgcag
    22621 tgcataacgg cctgaccgct atcccggacg cgcatcacta ttatcacggt gaaaaagtgg
    22681 cattcggtac gctgacgcag ctggttctgg aaaatgcgcc ggtggaggaa atcgaaaccg
    22741 tagctgccct tagccatgcg gtaggtttgc caataactct cgctcaactg gatattaaag
    22801 aagatgtccc ggcgaaaatg cgaattgtgg cagaagcggc atgtgcagaa ggtgaaacca
    22861 ttcacaacat gcctggcggc gcgacgccag atcaggttta cgccgcactg ctggtagctg
    22921 accagtatgg acaacgtttc ctgcaagagt gggaataacc tactccaaac tcccggcttg
    22981 tccgggagtt tgaacgcaaa attgcctgat gcgctacgct tatcaggcct acgcaatctc
    23041 tgcaatatat tgaatttgcg tgctttttag gccagataag gcgttcacgc cgcatccggc
    23101 actctgtgcc agtaatctga aactcccacg ttgttcagga attttttatc cgcttctgga
    23161 cattttctcc acagaacaat cgttagcccg gaaggtcgaa tctgtgactc ttattttcac
    23221 ggacgaacca gatgaaagct tcactggcgc tgctcagcct gctaacggca tttaccagcc
    23281 actccttaaa atctcccgcc gttccaccaa ccgtggtaca aatccaggcc aataccaacc
    23341 ttgccatcgc agacggtgcc agacagcaaa ttggcagcac gctattttac gatccggcgt
    23401 atatgcagct tacctatcct ggcggtgatg ttccgcaaga acgcggtgta tgttccgatg
    23461 tggtgatccg tgcattgcgc agccagaagg tcgatttgca gaaactggtt cacgaagata
    23521 tggcgaagaa ttttgccgat tatccgcaaa agtggcagtt aaagcgcccg gacagcaaca
    23581 tcgatcaccg tcgggtgcct aatctggaaa cctggtttac ccgccacgat aaaacacgcc
    23641 ccatcagcaa gaaccccagt gactatcaag cgggcgatat tgtctcctgg cgattagata
    23701 acgggctggc acatattggg gtggtttcag atggcttcgc ccgcgacggt acgccgctgg
    23761 tgatacataa cattggcgca ggtgcgcagg aagaagatgt gctgtttagc tggcgcatgg
    23821 tggggcatta tcgttatttt gttaaataag cgatcgcccc acatgaagaa aaaattcaga
    23881 tcctttttac actaacggct tcttttttct tacggctcac cagcgccagt gccagcacaa
    23941 tcagcacaat accgctacct tcaacggccc ccggattctc accgagcagc caccaggaaa
    24001 agagtacgcc gcaaacggga acggccagtg tgcttaagct ggcaatactg gcaggcaagt
    24061 ttttcagcac aaacagccat aagctccacg ccaacgccgt cgccagaatc gcactgtagg
    24121 ccagcgccca gaacacggtg ggctgccagt caatttcacg ttgcggcacc agtaaagcga
    24181 ccacactcat caccagcgcc gcatagagca tctgccagga tgttaacgac aataaatcca
    24241 cgcgcggatg acgggcatac agacgtttag caacaatcgc gctcgccccc caactaacac
    24301 cggagaggat tgccagcatg gcacttttca tcgaagagaa atcgagttgc cacggctgca
    24361 acaccaaaaa taagccgaaa gcggcaatca gtatcgcgaa atattgccca cgtcgcaggc
    24421 gttcaccaag aaacaacgcg gcgaaaatca ccacccagaa cggcatggta tagctcagga
    24481 tcgccacctt ccccgctccg ccgctgacca gcgcccactg cgccaggcca accatcccgc
    24541 aggtttgtaa cagggcaatg gctaaggtgt atttaaacgg cgtcgggcgc attccgcgac
    24601 cacgtaataa aaggacgatg aataaaacga gagcgccgaa aatgcagcgt aaggcggtaa
    24661 agtcgaaggc accgatgtag ctggtgactt gcttcatgaa aatccagcta taactccaga
    24721 taagtgtgag cacaaccagg ccactgattg ccagtgggtt gctctttcct gcggcagaca
    24781 tgagagatcc agtgtgtagt tttcctgtgg acactatacg ccggtaaaga aacttcagcg
    24841 actgcctgaa aggcagtcgc tgaagaaggt cagattacag caggtcgaaa cggtcgaggt
    24901 tcatcacttt cacccatgcc gccacgaagt ctttaacaaa cttctcgtga gcatcgctgc
    24961 tggcgtaaac ttccgctact gcacgcagga cagagttaga accaaatacc agatccgcac
    25021 ggctggctgt gtatttcact tcgccggttt cgcggtcacg gccttcgaac agctctttcg
    25081 attcgtcggt cgctttccac tcgtaacgca tatccagcaa gttcacaaag aagtcattgc
    25141 tcaatacgcc aacgcggtca gtgaagacgc cgtttttgct gccatcgaag ttggcaccca
    25201 gtacacgcat cccgcccacc agcgcggtca tttccggcgc ggtcagggtc agttgctggg
    25261 ctttatcaat cagcagtgac tcggtggtgg aaacgtccag acgagcgcgg tagttacgga
    25321 aaccgtcagc aattggctcc agcagctcaa acatttcaat gtcagtctga tcctgacgcg
    25381 catcaacgcg acccggcgca aacggtacat gaatgctcaa accagcggcg cttgcggctt
    25441 tctcaacgcc aaccacacct gccagcacga tgatatccgc cagcgaggct ttaccagact
    25501 ctttctgaat tttctccaga acaggcagag cacgaacggc tgctgcgttc acatcccagt
    25561 cgcgctgcgg cattaatgcc agacgcgcac cgttggcacc accgcgtttg tcgccaccac
    25621 ggaaggtaga agcagaagcc caggccaccg ataccagctc actaacagac agaccagaat
    25681 ccgcaatcgc gaatttcaga tcgataatgt cctgctcggt cgggttgtag atcggctgcg
    25741 gcagcggatc ttgccagatc agatcttctt tcggcacttc cggcccgatg tagcgagatt
    25801 tcggccccat atccctgtgc gtcagtttga accaggcacg ggcaaaggct tcgttgaacg
    25861 cctgcggatc gttgaggaaa cgacgagaga tcttctcgaa ctcaggatca aagcgcagcg
    25921 tcaggtcggt caccagcatc gtcggtttac gtttcttcga cggatcaaac ggatccggaa
    25981 taatttccgg tgcgtctacc gcttcgaact ggattgcgcc agccgggctg cgggtctgta
    26041 cccactcata cttgaacagg ttctcgaaga aatagttgct ccactgggtc ggcgtctggg
    26101 tccagactac ttccagacca gaggtaatgg catctgcgcc aacgccgctg ccgtaagtgc
    26161 tcgcccaacc taaaccttgt tcttcaatcg gtgcagcttc tggatcagga cctacatttg
    26221 atgtcggacc agcaccgtgg gttttaccca gcgtatgacc acccgcaatc agcgccactg
    26281 tttcttcgtc gttcataccc atgttgccga aggtcgcgcg gatagctgct gccgcagaaa
    26341 gcggttcgcc gctgtgatca gggccttccg ggttaacgta aatcaggccc atctcggttg
    26401 cgcccagcgg tgctttcgcc agcgcttccg gatgacggtg agtcagccag gctttttcat
    26461 caccccagtt aacatccaga tccggttccc agacgtcttc acgaccggca ccaaaaccga
    26521 aggtacggaa gccggagttt tccagcgcca cgttacccgc gaggataaac aggtcggccc
    26581 aggagatttt ctgaccatat ttctgtttga ttggccacaa caggcgacgc gctttatcga
    26641 ggcttacgtt atccggccag gagttcagcg gtgcaaaacg ttgctgacca cgacccgcgc
    26701 cgccgcgtcc atcgattgaa cggtaagtac ccgcgccgtg ccaggccata cgaataaaca
    26761 gaccggcgta actgccccag tcggcaggcc accacggctg agattctgtc agcagggctt
    26821 tcagatcttt tttcaggccg tagtaatcta atttgctgaa ttctttgcgg tagtcaaagt
    26881 cctcacccag tgggttagaa cgattagaat gttggtttaa caggtcaaca cgaagttgat
    26941 ttggccacca gtcgcgagtg gttgtgcccg cccccgcact ctggtcgtga ccgccctgat
    27001 ggaatgggca tttgccagtg gctgtcgtgt tatggatatc gtctgacgtg ctcatcaatg
    27061 tgctcccctc tacagtgtta ccgttacgat acacagcgtt agagagaagt tataattgaa
    27121 ttaatccacg gatgcgatag ttgagatctt acatattgtt ggttaaagag atgtagatca
    27181 aattgatcgt aattaagctt ttttgattat gtccgaattc ggacaaatgc tttataaaaa
    27241 agggttttag cttcatatcc ttcaagtaat gagggagaaa acaaggctga taaccttatt
    27301 ttccagccct catttcgagg cagcattttg tgctctgttt aaaatttgtg atcactgtgt
    27361 gattttcaca aaagccacac tatttataaa ccaggtcgaa cccccagcgt atggcaaatc
    27421 gcgtaactca tttcagcacg gttaagcgta tagaagtgga aatctttcac tccttcacgg
    27481 cttaaaatct tcaccatatc catggcaata ttcgcgccaa ccagtttgcg ggtttcggca
    27541 tcatcatcca gaccgtcgaa catttgcgcc atccacgccg gaatacgtac gttggtcata
    27601 tcggcaaatt tcttcgcctg tttaaagtta gataccggca aaattcccgg aataatttcc
    27661 acatcaatgc ccgccgatac acagcggtca cgaaaacgca ggtagctttc gacatcgaag
    27721 aagaactgag taatcgcgcg gttggctccg gcatcgactt tgcgtttcag gttaagcaaa
    27781 tccgcctgag cgctttttgc ttctgggtga acttccggat acgccgccac ggagatatcg
    27841 aaatctgcca cttcttttaa cagcgtcacc aggtcagaag catacatttc tggcttacca
    27901 cttcccggcg gcagatcgcc acgcagcgcc acgatatgac gaataccgtt attccagtag
    27961 tcgcgtgcaa tggtgcgcaa ctcgtcgggc gtcgcatcaa tgcaagtaag atgcggtgcc
    28021 gcttccagac cagtgcgatc tttaatgcct ttaataatgc tgtgcgtacg gtcgcgctcg
    28081 ccggagttcg cgccataggt caccgataca aacttcggtt tcaggctgct aaggcgatcg
    28141 atggagttcc acagggtctg ctccatttca ctggtacgcg gcgggaaaaa ctcgaacgaa
    28201 acgttaatct gcccctggac ttctgccagg ctctgattca gggcatcccg ctggctggcg
    28261 tgaaaaaagc tcatacctta cctcatcaat cgcttgtagt tatatgttgt gttgtgcaca
    28321 tctatccgtt tagacgtcca gatgtaaaga tgaaggaaaa gccgaagggc gtcaaccgaa
    28381 aaatgaccaa tgatggtgag gaaaaatcag ggaagatgaa aaaacttcag gatggtaaga
    28441 aaaagaaaat ccggtatgca aagcaaaccg gactgcaaat attaactaac catgtactta
    28501 ttgcttcttg ccggattcgt cgtaaacgct tcatccggcc taatcctatg ataattcaat
    28561 atatttcatt agaaaccata gacccgaaaa catgagtacc gggcattatt aaatttctga
    28621 aattacaaca actgtgccag ccggttgata tccgactgaa tcgccccggc ggtgacgtcg
    28681 cgccccgcgc caggcccacg gatcaccagc gggttgtcgc gataccagcg gctttcgatg
    28741 gcaaagacgt tatcgcacgg cagcagtgat gccagcggat ggtcttcacg caccgcctcc
    28801 acgcccacac gcgctttgcc gttggcatcg aaacgcgcca cgtagcgcag caccagtccc
    28861 atttcgcggg ccgcttccag ccgttgcacc atctgctcgt taagttcatc gccattttca
    28921 aagaaatggt cgatgctgcc gccttcgcaa tgagcaggca ccagcgattc cacacgtacc
    28981 tggtccggtt caatgttgta acccgcttca cgcgccagaa tcaccagctt gcgcattacg
    29041 tctttgccgg agaggtcgtc acgcggatca ggctcggtta agccctgctg ccacgcctga
    29101 tccaccagct cggtaaacgg cacgctaccg tcgaattgca ggaacagcca ggagagcgtg
    29161 ccggagaaga tcccactgat cgacaaaata gtatcgccgc tgtcgatcag atcgcgcacg
    29221 gtgtggttga tcggcaagcc cgcaccgacg gtggcgttgt acagccagtg acgcccggtt
    29281 ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt cgcttgctcc cgccagtttg
    29341 ttggcactga tgacgtggaa accgtggctg gcgaaatcaa gatactggtc agccagctgc
    29401 tggctggcgg taacgtccag caccactaaa tcatcatacg gatgggcgcg catccacagg
    29461 aaaagcgact cttcatcctg ctcgaccgct tcatcgttga agaaggctaa cgcgcggctg
    29521 gcgtccagcc cgtcataact caacagactg cggcggctgt ccaccacgcc tgccagcaca
    29581 aattcaaagc cggtacgcgc cgaaagcgag ctctgctcgc gggcgaacag ttccagccag
    29641 cgggaaccga tattgccttt gccgaacaat accaggccga tgcgtttttc tgcgcggaag
    29701 acggactgat gcaggccctg aatcaggctt tcggtcgggc cggtgcgcag tactgccacc
    29761 aggctgatgc cgtcatcgga ctgccaggta aactcgaccg gctggccttt cagttgctgc
    29821 cagaagcggt ggcaatgcag cgggttacgg gtgacacccg ccccgaccat cgccaccagc
    29881 gccagcccct gacgcaggcg cagttcgcca ggtaatcccg cttcgtcgag gattttcagc
    29941 gcactgtcgg ccacttctga ggtgtagcaa aattgcagca actggcgatc gttatgcacg
    30001 ccaaccgcca gtggacgcac ctgggcacgt ttcaggattt ggtcgatctc tttatgcgcc
    30061 agtttgaaat cctgactggc gggcacctga aactcaatca aacagacatc atcgtggctg
    30121 gtgacaatac gcgcaccagt accggaggcc agcacgcgtt caatgcgcgt ggaaccttga
    30181 tccggcgtgt agctacagcg cagctgcagg tcgatttcgc tgccagaaac tggctgtaaa
    30241 gtacgggcgt gaagaacggg agccgccaga cgcgccagtt cgctggcctc atccagacgc
    30301 agcaacggca gcaggcaggc atctttcact ttacgcgggt cggcactgta taccccggcg
    30361 acgtcgctcc agatggttac gcgagaaaca cccgccagcg caccgatttg tgtcgcggaa
    30421 tagtcggaac cgttacgccc cagcagcacc gtttcaccgg cgttgttacg gctgataaac
    30481 ccggtcacca ccagacgttt acccggatgt tgtaccagta gctgttgcag caacgggtaa
    30541 gaaagccctt catcaacctg cggttgcgca gcgcgttcag cgcgtaaaaa ctcgcgggca
    30601 tcaagccagg ccgctggcag cccttgttga ttaagtaccg cagacatcag tcgcgccgac
    30661 cacacttcac cgtggcccac cacttccgca tacactgcgt cgttaatacc gctgtcgagc
    30721 agcgccgcca ggcgctcaag atcgctgaca aaagcgctaa tgaggctatc ggcttcttcg
    30781 gcgggtagca ggccgctaat cagatcgcac tgatagcgac gcagcgtttg ttgaacctga
    30841 tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt taatcaactg gttagtggtg
    30901 ctaccggcgg cggaaaccac catcatatcg tcaggctgag agtactccgc cataatgccc
    30961 gcgacacgca aataacactt cacatcagcc agactactgc caccaaattt atgcagctga
    31021 cgacctttcg cccctgcctg cgcaatcaca ctcattttta ccccttgttt gcagcccgga
    31081 agccattttc caggtcggca attaaatctt cgccatcttc aataccggtg gagatacgca
    31141 gcagcgtctc ggagatcccg gcggcagcac gcgcttctgg cgccatgcct gcatgtgtca
    31201 tggttgcggc gtgagagatt aaactttcca ctccccctaa cgattccgcc agcgtaaaca
    31261 acgacagccc gcccaggaaa cgacgcagcg tctgctcatc gccatccagt tcaaaactca
    31321 acattgcgcc aaagcctttt tgctgacgcg cggcaatttc atgcccctga ttttccggca
    31381 gcgacgggtg atacagtttt ttcaccaacg gctgggtttg cagatatttc acaatcgcct
    31441 gcgcgttgcg ctgcgccagc tccatacgcg gcaccagcgt tcgcaacccg cgtagcagca
    31501 gatagctgtc aaacgcgccg cccgtcacgc caatattgtt tgcccaccag gcaagttcag
    31561 tgacaacgtc cgggtcttta gcaatcacca cgccagccac cacgtcagag tgaccgttca
    31621 gatatttcgt acatgaatgc aacaccagat cggcacccaa tgccagcgga ttttgtaatg
    31681 ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc gacttccctt gccagatggc
    31741 agattttcgc aatatccacg acgcgtaaca atggattact tgggctttct accagtacca
    31801 gtttggggtt ttctgccagc gctgcccgta atgcctgttc atcgccttga tcaacaaaca
    31861 acacgcgata gcaaccgcgt ttcgccaggc tgtcgaacag gcgatagcta ccgccgtagc
    31921 agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa gacggtcgtt accaggtgaa
    31981 tcgcggacat gccggtatta gtaagtactg caccagcacc gccttccagt tctgccagcg
    32041 cacgctgaac cacatcgcgc gttgggttgc cgcgacgcga gtaatcatgc gcgcgcggtt
    32101 cattaaatcc ggtaaagtta taggtgctgg aaagatggat gggagggaca acgcaaccat
    32161 actgttcgtc gtcatttaac ccgctacgca ctgcgatggt ggcctgttta cgcgtcatgt
    32221 gatgaagttc cctgggcttg gtcggtgaaa tgtcaggcac cagagtaaac attgtgttaa
    32281 tggacgtcaa tacatctgga catctaaact tctttgcgta tagattgagc aaatcccaaa
    32341 tagccgttaa aattatatgc attatcacgc cgacaggtgc attacacgat gtcacggtaa
    32401 cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc acataaattg tgtggtctgg
    32461 tctcaattta ttgacgaaga ggattaagta tctcatggct gaatggagcg gcgaatatat
    32521 cagcccatac gctgagcacg gcaagaagag tgaacaagtc aaaaagatta cggtttccat
    32581 tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg cgtcgtcagg tgaacaacct
    32641 gcgtcacgct accaacagcg agctgctgtg cgaagcgttt ctgcatgcct ttaccgggca
    32701 acctttgccg gatgatgccg atctgcgtaa agagcgcagc gacgaaatcc cggaagcggc
    32761 aaaagagatc atgcgtgaga tggggattaa cccggagacg tgggaatact aaagcggaaa
    32821 agagcggcgc ggagtggaat cgcctgatgc gctacgctta tcaggcctac gtcatattgc
    32881 aatttattga atttgcacga acttgtaggc cggataaggc gttcacgccg catccggcat
    32941 aaacaacgag cacgttgtct gcgacccacc gctttttata catggacgtt taacgatgaa
    33001 atacaggcta ctgatcctca gtctgctggt ttctgtgcct gcctttgcct ggcagccgca
    33061 aactggcgac atcatctttc aaatctctcg ctcatcgcaa agtaaagcga tccaactggc
    33121 gacccattcc gattacagcc acaccggtat gctggtgatg cgtaacaaaa agccctacgt
    33181 ttttgaagcg gtcggcccgg tgaaatacac cccgctcaag cagtggattg cccatggtga
    33241 aaagggcaaa tacgttgttc gccgcgttga aggcggactg agtgttgaac aacagcaaaa
    33301 actggcgcaa acggcgaaac gttatctcgg caaaccgtac gatttcagct tctcatggag
    33361 cgacgatcgc cagtactgtt cggaagtggt gtggaaggtt tatcagaatg cgctgggaat
    33421 gcgcgtgggc gaacagcaga agttgaaaga gtttgatctc agcaacccgc tggtccaggc
    33481 gaagctcaaa gaacgctacg gcaaaaatat tccgcttgag gaaacggtgg tctcaccaca
    33541 agccgttttc gatgcgccac aactcaccac ggttgccaaa gagtggccgc tgttttcgtg
    33601 gtaatacgac caaaattcag gcacaaaaaa agcgccgtgc ggcgcttttt tcggaaatcc
    33661 ggtcttattt gctgcccggg atgttgaaac gcttgttgaa gcggtcaaca cggccaccgg
    33721 tagcaacatc acgctgtttg ccagtgaaga acgggtggca cttgctgcac acgtcgaggt
    33781 tcaggtcatg accaacggtg gagcggattt tcattacgtt accgcaagag cagctagcag
    33841 taatttcttc gtatttcggg tgaatatctt ttttcatggg aaaacctcgg tttaaggccg
    33901 cgtcgctctt ccagccctaa cgccagacac cacgcgatgt taaaagtata gcttcaatac
    33961 gatcatttcg tacgaaggcg cgaaatcata cagaaattaa ccagcgtatg caaactgatc
    34021 cgcactcttc tacggcaatg tgtatactaa cccaccgaat ttcaagtcag gatgatgcta
    34081 tgcccgttgc ccacgttgcc ttgcccgttc cgcttcctcg tacctttgac tatctgctgc
    34141 cagaaggcat gacggttaaa gctgggtgtc gcgtgcgcgt gccgtttggc aaacagcagg
    34201 agcgcatcgg gattgtggta tcagttagcg atgccagcga actgccgctc aatgagctaa
    34261 aagcggtagt cgaagtgctg gatagtgagc cggtgtttac tcactccgtc tggcgattgc
    34321 tgctatgggc ggcagattac tatcatcatc cgattggcga tgtgctgttt catgccttgc
    34381 cgattttact acgccagggg cggcctgcgg cgaacgcgcc gatgtggtac tggtttgcca
    34441 ctgaacaagg tcaggcggtg gatctgaaca gcctgaaacg ttcgcccaag caacaacagg
    34501 cgctggcagc gttacggcaa ggcaaaatct ggcgtgatca ggtcgcggaa cttgaattta
    34561 atgatgccgc gttgcaggcg ctgcgcaaaa aaggtctgtg tgatttagca agtgaaacac
    34621 cagtgtttag cgactggcga acgaactatg ccgtttctgg tgaacggttg cgattgaata
    34681 ccgaacaggc caccgccgtt ggcgcaattc atagcgcggc ggataccttc tctgcctggc
    34741 tgctggcggg cgttacaggt tccggtaaaa cagaggttta tttgagcgta ctggaaaacg
    34801 tgctcgctca gggcaaacag gcgctggtga tggtgccgga aatcggcctg acaccgcaaa
    34861 ctatcgcccg ttttcgtgaa cgttttaatg cccccgtaga agttctgcat tccggcctga
    34921 acgacagcga gcgtctttcg gcgtggttga aagcgaaaaa tggtgaggcg gcgattgtga
    34981 tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct cggtgtgatt gtcattgatg
    35041 aagagcacga cagttcctac aagcagcagg aaggctggcg ctatcatgcc cgcgacctgg
    35101 cggtgtatcg cgcgcacagc gagcaaatcc cgattgttct tggctccgca acacccgcgc
    35161 tggaaacgtt gtgcaacgtc cagcagaaaa aataccgcct gctgcgcctg acgcgtcggg
    35221 cggggaatgc gcgtccggca attcaacacg tgctggattt aaaaggtcag aaggtgcagg
    35281 cggggctggc tccggcgtta atcacccgta tgcgccagca tttacaggcc gacaaccagg
    35341 ttatcctctt tcttaaccgc cgtggctttg cgcctgcctt gctgtgccac gactgcggct
    35401 ggattgccga atgtccacgt tgcgatcact actacacgct gcatcaggcg caacagcatt
    35461 tgcgctgtca ccactgtgac agccaacgcc cggtgccgcg ccagtgccct tcctgcggtt
    35521 ccacgcacct ggtacccgtg gggctgggca ccgaacagct tgaacagacg ctcgcgccgt
    35581 tattccctgg tgtgcccatt tctcgtatcg accgcgatac caccagccgc aaaggagcgc
    35641 tggaacaaca actggcggaa gtgcatcgcg gcggcgcgag gatcttgatt ggcacgcaaa
    35701 tgctggcgaa aggtcaccac tttccggatg tgacgctggt agcgttactg gatgtggacg
    35761 gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt tgcccagcta tacacccagg
    35821 tagcaggccg tgccgggcgt gcgggtaaac agggcgaagt ggtgctgcaa acgcatcatc
    35881 cggaacaccc gctattgcaa acgttgctct ataaaggcta cgacgctttt gccgaacagg
    35941 cactggctga gcgacgcatg atgcagctgc cgccgtggac cagccatgtg attgtgcgtg
    36001 cggaagatca taacaatcag catgcgccgc tgttcctgca acaactgcgt aatctgatcc
    36061 tctccagccc gctggcagac gacaaattgt gggttctcgg tccggttccg gcactggcac
    36121 ctaaacgtgg cggtcgctgg cgctggcaga tattgttgca gcacccttcc cgcgtgcgct
    36181 tgcaacacat cattaacggt acgctggcac tcatcaatac aataccggat tcccgtaagg
    36241 tgaaatgggt gctggatgtt gatccgattg agggttaaac cgctcacgat gcgaggcgga
    36301 tcgaaaaatt caatattcat cacacttttc atgaaaattc tgtaaccgtt ttcacgcgct
    36361 atctgctaaa aatgttgccg atgtgaagta aacatggatg tagtacgcct gacgtgccag
    36421 gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc gcgaccatga aagacgttgc
    36481 cctcaaggca aaagtctcta cagcgaccgt ctcccgagca ttaatgaatc ccgataaagt
    36541 ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg gaagtgggtt atttaccgca
    36601 gcctatgggg cgcaacgtca agcgtaatga atcccgcacc attctggtga ttgtcccgga
    36661 tatctgcgat cccttcttta gcgaaattat tcgcggtata gaagttacgg cggcaaatca
    36721 cggatatctg gtgctgattg gcgactgtgc gcatcaaaat cagcaggaaa aaacctttat
    36781 cgatttgatc atcaccaagc aaattgatgg catgttgctg ctgggttcaa ggctgccgtt
    36841 tgatgccagc attgaggaac agcgtaatct gccgccgatg gtgatggcga acgaatttgc
    36901 accggaactg gagctgccta ccgttcatat cgacaatctg accgccgcat ttgatgcagt
    36961 aaattattta tatgagcaag ggcataaacg gattggctgt atagccggtc ccgaagagat
    37021 gccgctgtgt cactaccgcc tgcaaggtta cgttcaggcg ctgcgtcgct gcggcattat
    37081 ggttgatccg caatacatcg cccgtggcga cttcaccttc gaagccggaa gcaaagcgat
    37141 gcagcagctg cttgatcttc cacaaccgcc tactgctgtc ttctgccata gcgatgtgat
    37201 ggcgctcggc gcactttctc aggcaaaacg ccaggggctg aaagtcccgg aagacctttc
    37261 cataatcggt tttgataaca tcgacctgac gcaattttgt gatccgccgc tgacaaccat
    37321 cgcgcagccg cgttacgaaa tcggtcggga agctatgctg ttattgcttg atcaaatgca
    37381 ggggcaacac gttggcagtg gctctcgttt aatggactgc gaacttatca tccggggatc
    37441 aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg tgactggtca aaggcccgcc
    37501 gcttaagtaa catggcgggc tgacgaacga ataaatacag cgaaacgata gtggcacaac
    37561 gagattatgt acgccgcagc caaccggcac cttcgcggcg aaaaaagagc acctcacgga
    37621 aaaagcaacg aaatctgcct gcggtttctc ccgctatggt cgctattgct gccgccgttc
    37681 ttgtgacctt tatcggtggt ctgtacttca ttacgcatca caagaaagaa gagtccgaga
    37741 cgctgcaaag ccagaaagtg accggaaacg gactaccacc aaaaccagaa gaacgctggc
    37801 gctacatcaa agagctggaa agtcgccagc cgggtgtgcg tgcgcccaca gaaccttctg
    37861 ctggtggtga agtgaaaacg ccggagcaac tgacaccaga acaacgtcag cttcttgaac
    37921 aaatgcaggc tgatatgcgc cagcagccaa cgcagctggt tgaggttccg tggaatgaac
    37981 agacgccgga acagcgtcag caaacgctac agcgccaacg tcaggcgcag caattagcgg
    38041 aacagcaacg cctggctcag cagtcgcgta cgactgaaca aagctggcag cagcagacgc
    38101 gtacgtcgca agccgcgccg gtacaggcgc aaccgcgcca gtctaaaccg gcttctactc
    38161 agcagccgta ccaggatctg ctgcaaaccc ctgcacacac gaccgcgcag tcgaagccgc
    38221 agcaagctgc gccggttact cgtgccgctg acgcgccaaa accgacggcg gagaaaaaag
    38281 acgaacgccg ctggatggtg cagtgcggtt cgttcagagg cgcggaacag gcagagacgg
    38341 tacgtgctca gctggcgttc gaaggatttg actcgaaaat caccaccaac aatggctgga
    38401 atcgtgtggt cattggcccg gtgaaaggca aagagaacgc agacagcacc ctcaatcggt
    38461 tgaagatggc gggtcataca aactgcattc ggctcgccgc cgggggttga aaccctcaaa
    38521 atccccccca tctataattg cattatgccc cgtacttttg tacggggttt gtactctgta
    38581 ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc gtacgccgta acggccatgt
    38641 ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc gtaatgaaag gcaacgtgaa
    38701 aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc tttgcgggcg gtactgcgga
    38761 tgcttttacg ctgttcgaac tgtttgaacg taaactggaa atgcatcagg gccatctggt
    38821 taaagccgcc gttgagctgg caaaagactg gcgtaccgat cgcatgctgc gcaaacttga
    38881 agcactgctg gcagtcgcgg atgaaactgc atcgcttatc atcaccggta acggtgacgt
    38941 ggtgcagcca gaaaacgatc ttattgctat cggctccggc ggcccttacg cccaggctgc
    39001 ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt gaaattgctg aaaaggcgtt
    39061 ggatattgca ggcgacattt gcatctatac caaccatttc cacaccatcg aagaattaag
    39121 ctacaaagcg taaggatctc ccatgtctga aatgacccca cgcgaaatcg tcagcgaact
    39181 ggataagcac atcatcggcc aggacaacgc caagcgttct gtggcgattg ctctgcgtaa
    39241 ccgctggcgt cgcatgcagc tcaacgaaga gctgcgccat gaagtgaccc cgaaaaatat
    39301 cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc gcccgtcgtc tggctaagct
    39361 ggcgaatgcg ccgttcatca aagttgaagc gaccaaattc accgaagtgg gctacgtcgg
    39421 taaggaagtg gattctatta ttcgcgatct gaccgatgcc gccgtgaaaa tggtacgcgt
    39481 ccaggctatc gagaaaaacc gttatcgcgc tgaagaactg gcagaagaac gtattctcga
    39541 cgtgctgatc ccaccggcta aaaacaactg gggacagacc gaacaacagc aggaaccgtc
    39601 cgctgctcgt caggcattcc gcaaaaaact gcgtgaaggc cagcttgatg acaaagaaat
    39661 cgagatcgat cttgccgcag caccgatggg cgttgaaatt atggctcctc cgggcatgga
    39721 agagatgacc agccagctgc agtctatgtt ccagaacctg ggcggtcaga agcaaaaagc
    39781 gcgtaagctg aaaatcaaag acgccatgaa gctgctgatt gaagaagaag cggcgaaact
    39841 ggtgaacccg gaagagctga agcaagacgc tatcgacgct gttgagcagc acgggatcgt
    39901 gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag tcttccggtc cggatgtttc
    39961 tcgtgaaggc gttcagcgag acctgctgcc gctggtagaa ggttgcacgg tttccaccaa
    40021 acacgggatg gtcaaaactg accacattct gtttatcgct tctggcgcgt tccagattgc
    40081 gaaaccgtct gacctgatcc cggaactgca aggtcgtctg ccaatccgcg ttgaactgca
    40141 ggcactgacc accagcgact tcgagcgtat tctgaccgag ccgaatgcct ctatcaccgt
    40201 gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc gagtttaccg actccggtat
    40261 taagcgcatc gcggaagcgg catggcaggt gaacgaatct accgaaaaca tcggtgctcg
    40321 tcgtttacac actgttctgg agcgtttaat ggaagagatt tcctacgacg ccagcgattt
    40381 aagcggtcaa aatatcacta ttgacgcaga ttatgtgagc aaacatctgg atgcgttggt
    40441 ggcagatgaa gatctgagcc gttttatcct ataatcgcgt tcaatcattt tcatcattgt
    40501 ttgatggggc tgaaaggccc catttttatt ggcgcgtatt atgactgaac aacaaattag
    40561 ccgaactcag gcgtggctgg aaagtttacg acctaaaacc ctccccctcg cctttgctgc
    40621 aattatcgtc gggacggcgc tggcatggtg gcaaggtcat tttgatccgc tggtcgccct
    40681 gctggcactg attaccgccg ggctattaca gatcctttct aacctcgcca atgattacgg
    40741 cgatgcagta aaaggcagcg ataaacctga ccgtatcgga ccgctacgcg gcatgcaaaa
    40801 aggggtcatt acccagcaag agatgaaacg ggcgctcatt attaccgtcg tgctcatctg
    40861 tctctctggg ctggcactgg ttgcagtggc atgccatacg ctggctgatt ttgtcggttt
    40921 cctgattctt ggcgggttgt cgatcattgc cgctatcacc tacaccgtgg gcaatcgtcc
    40981 ttatggttat atcggtctgg gtgatatttc cgtactggtt ttctttggct ggttaagcgt
    41041 catggggagc tggtatttac aggctcatac attgattccg gcactgatcc ttccggcgac
    41101 cgcatgcggc ctgctggcaa cggcagtact gaacattaat aacctgcgtg atatcaatag
    41161 cgaccgcgaa aacggcaaaa acacgctggt ggtgcgctta ggtgaagtga acgcgcgtcg
    41221 ttatcatgcc tgcctgctga tgggctctct ggtatgtctg gcgctgttta atctcttttc
    41281 gctgcatagc ctgtggggct ggctgttcct gctggcggca ccgttgctgg tgaagcaagc
    41341 ccgttatgtg atgcgggaaa tggacccagt ggcgatgcga ccgatgctgg aacgcactgt
    41401 caagggagcg ttactgacta acctgctgtt tgttgtaggg atattcctaa gccagtgggc
    41461 agcataactg acaaatatca attaacaatt gatgattttg ccaacagacc acatagcgcg
    41521 atatactgaa aattctcgca gcaactgaat gttaagccta tgaaatacga tacttccgag
    41581 ctttgtgaca tctatcaaga agatgttaac gtcgtggaac cgctgttctc caactttggc
    41641 ggacgggcgt cgtttggcgg acaaataatc acggtaaaat gtttcgagga caacgggttg
    41701 ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc ttgtcgtcga tggcggtggt
    41761 tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc tggcagtaca aaatgaatgg
    41821 gaaggtctgg tcatttacgg cgcggtgcgt caggtagatg acctggaaga gttggatatc
    41881 ggcatccagg cgatggcggc aattccggtt ggtgccgctg gcgaaggcat tggcgaaagc
    41941 gatgtccgcg tcaattttgg cggtgttacc ttcttctccg gcgaccatct ttatgccgac
    42001 aataccggga ttattctttc agaagatccg ctggatattg aataagtatc tgccatgcgt
    42061 taaaagggga attaaattcc ccttttttat acatggacgt attcatgcag aatattattc
    42121 ttttatttat atatttatta cttatagccg ttaaccattt ccgctacaaa tttcttttat
    42181 ctggcaacgc gggtgaaatg attttatatt ttgctaacgt attttttaac ccacttgcct
    42241 tatcatttat tatcgccaca atcatctgta taacgataaa aaaaagaagc agccgcagct
    42301 ttattcgcgg cacttgttgg ttaatgggat tatttcttat ctatagcagc tatacggtgt
    42361 gggaacgaca cagcatatgg gattatactt tcccggagcc aggtatcacc gtcacggttc
    42421 cgagcaaaca gtgggttgcc aatatagtaa aacaaggacc aaccctggtg acccgggatg
    42481 cccatgctat tttagctatt gttgccttct cacaaaatga actcggtgtg cattcaatcg
    42541 aggaacttac cgcgactcaa aacggaactg cagaaatgca tgtttgtgat gttcagggat
    42601 ttgcctgcgc ttatcaggaa ggagtgcaaa caatgagtga tggcaaagta agacatgcta
    42661 ttttcatgac tctactggat aacactaatt tgatacaact ggtcgcagct atcgacccag
    42721 attatttgga cgaatatcaa gaagaagtaa tgaaaatgat gctttctgct cgtcaatgat
    42781 ctgatgtgca tgttaataat aatggattga tatgagatac gttatcttat taatactatg
    42841 gataataact gtttttctta gtcatcttca gcacagtaac ttgcttactg gagatcctgg
    42901 gatggttggc gagtatatcg gttcagtact tcttgggccg ttattacttc ccgtgcttgt
    42961 gtcaggtatt ctgtgcacat tcactaaaaa aacgcgtaac ttcgccagtt ttactcgcgg
    43021 gtgctgttgg gtattgggag tcctgctttt atccaacgtc ggcaatacgt ttcgtctgtt
    43081 caccccgtgg cactatacgt ttgagaaggc agctatttcc gtgactgtcc caaaccgaca
    43141 ctggaatacg gtttctatta gtactgataa aaccattgat ataagaagtg aagataatag
    43201 cgtctttatt tctgctttcc gcctgcccgc aggacgtagc gccgatgact cgctggaaga
    43261 gttaaagaag atgcagcgag acaatctcaa ggatcaatac aatgaagaaa ccttccagtt
    43321 tcatgattgt aatgccaaac atttcacctg taagtatcag gatgtgttga taaattttga
    43381 tggtcagcag aaaagaacca tcagtgttta tcttgaagac acccccaggg ctgtggggat
    43441 cattgcatta atggagccag atacggcaga taaatatcgc caacaggcga tggagattat
    43501 gttgtctgct aaaaatacag tgaagtaaaa aataaggcgc cttgcggcgc ctctgcgtgg
    43561 aagagattaa acctcttcca tgcgacccag cagggcctgc agacgttcct gccagccgtt
    43621 ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat gctgggcatt
    43681 ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt ccatctgcaa
    43741 cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa acacttctaa
    43801 tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat cctcgtcccg
    43861 attaccggtg acgccttaat aaatacgagc gaactttagt tagctccgat tgtatgaagc
    43921 cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac ttttcatcct
    43981 tctcctggtg acataatcca caccaatcga aaatgttaat aaatttgttg cgcgaatgat
    44041 ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac gctcatttta
    44101 tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta acgataagtt
    44161 tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa caaacattaa
    44221 ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc attgctgaat
    44281 tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg
    44341 ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg gtggcaatgg
    44401 ccatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt accattgcat
    44461 tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt tcacaagttg
    44521 ccggcgcttt ctgtgctgcg gctttagttt acgggcttta ctacaattta tttttcgact
    44581 tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg gcaggcactt
    44641 tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt gagatggtga
    44701 ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac
    44761 gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc gcatctatgg
    44821 gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa gtctttgcct
    44881 ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct tacttcctgg
    44941 tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac cgcaaactga
    45001 ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga aaaggagacc acaactcctt
    45061 cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac tgaaaaaaaa
    45121 tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat ggatcacgat
    45181 gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa accaggctgg
    45241 gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt agaagtgctg
    45301 gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa
    45361 accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat tgtctggcag
    45421 tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga agattatatc
    45481 cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt gaagtggatc
    45541 ctcgaccatg tggaaggctc tcgcgagcgt gcgcgtcgtg gtgaattgct gtttggtacg
    45601 gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac cgattacacc
    45661 aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga caaaatgctg
    45721 gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc cgaagtgtac
    45781 ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg gatcgccggt
    45841 gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc gaagaacacc
    45901 tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa atcagaaaac
    45961 ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc gttggaaggt
    46021 gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa gttgattaac
    46081 gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg
    46141 gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt
    46201 ttcggtctga ctcgtggggt aaacgctaac cacattatac gcgcgacgct ggagtctatt
    46261 gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat ccgtctgcac
    46321 gccctgcgcg tggatggtgg tgcagtagca aacaatttcc tgatgcagtt ccagtccgat
    46381 attctcggca cccgcgttga gcgcccggaa gttcgcgaag tcaccgcatt gggtgcggcc
    46441 tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca agagaaagcg
    46501 gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta ccgttacgca
    46561 ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga gtaatgtcag
    46621 gcacctgaat cgattgcctg atgctgatcg cctgatgcga cgcttacgcg tcttatcagg
    46681 cctacgagcc aatgccattt tgtgtaggcg ggataaggcg ttcacgccgc atccggcatc
    46741 acgcagactc tgaaaccgtg cgggggcaac cccgcacaca tcaataatcc cccccttccc
    46801 ctgtgctaca cttcgcgcca ttccttactg cttagagttt gctatgagac gagaacttgc
    46861 catcgaattt tcccgcgtca ccgaatctgc ggcgctggct ggctacaaat ggttaggacg
    46921 cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc atgcgtatta tgctcaacca
    46981 ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa atcgacgaag caccgatgct
    47041 ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg gtagatattg ctgttgatcc
    47101 gattgaaggc acgcgcatga cggcgatggg ccaggctaac gcgctggcgg tgctggcagt
    47161 aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat atggagaagc tgattgtcgg
    47221 gccgggagcc aaaggcacca ttgatctgaa cctgccgctg gcggataacc tgcgcaatgt
    47281 tgcggcggcg ctcggcaaac cgttgagcga actgacggta acgattctgg ctaaaccacg
    47341 ccacgatgcc gttatcgctg aaatgcagca actcggcgta cgcgtatttg ctattccgga
    47401 cggcgacgtt gcggcctcaa ttctcacctg tatgccagac agcgaagttg acgtgctgta
    47461 cggaattggt ggcgcgccgg aaggcgtggt ttctgcggcg gtgatccgcg cattagatgg
    47521 cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa ggcgacaatg aagagaatcg
    47581 tcgcattggc gagcaggagc tggcacgctg caaagcgatg ggcatcgaag ccggtaaagt
    47641 attgcgtctg ggcgatatgg cgcgcagcga taacgtcatc ttctctgcca ccggtattac
    47701 caaaggcgat ctgctggaag gcattagccg caaaggcaat atcgcgacta ccgaaacgct
    47761 gctgatccgc ggcaagtcac gcaccattcg ccgcattcag tccatccact atctggatcg
    47821 caaagacccg gaaatgcagg tgcacatcct ctgattgatt tgatcgattg agccttccag
    47881 tccttcggga ctggaatttt tttgttcgga gaacgaagat aaggcaagtc aatcaaaaca
    47941 ggagaaaaac atggctgatt gggtaacagg caaagtcact aaagtgcaga actggaccga
    48001 cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg tttaccgccg ggcaattcac
    48061 caagcttggc cttgaaatcg acggcgaacg cgtccagcgc gcttactcct atgttaactc
    48121 gcccgataat cccgatttgg agttttacct ggtcaccgtc cccgatggca aattaagccc
    48181 acgactggcg gcactgaaac cgggcgatga agtgcaggtg gttagcgaag cagctggctt
    48241 ctttgttctg gatgaagtac cagattgcga aacgctatgg atgctggcaa ccggtacagc
    48301 gattggccct tatttatcga ttctgcaact aggtaaagat ttagatcgct tcaaaaatct
    48361 ggtcctggtt cacgccgcac gttatgccgc cgacttaagc tatttgccac tgatgcaaga
    48421 actggaaaaa cgctacgaag gaaaactgcg cattcagacg gtggtcagtc gggaaacgac
    48481 ggcggggtca ctcaccgggc ggataccggc gttaattgaa agtggtgaac tggaaagcgc
    48541 ggttggcctg ccgatgaata aagaaaccag ccatgtgatg ctgtgcggca atccacagat
    48601 ggtgcgcgat acgcaacagt tgctgaaaga gacccggcag atgacgaaac atttacgtcg
    48661 ccgaccgggt cacatgacag cggagcatta ctggtaagcg gttacttatc gataaacggc
    48721 acgatgagca aatccgcact caacttattg atcatcccgc gatatgccgg catcaaacgg
    48781 ttgataaatg agtggtgatg accacagaca aggaggtcgc actgctcttt ttgcataatt
    48841 tccagcagtg tttccggcat ttctccgcgt tcaatacgca gttttgtctt cggccattga
    48901 atatttttcg tcagtttata cagcttgtta tccgacttat tcttcaacaa ttgaagaata
    48961 tcttctgttg cagggaagta gatacccgga tacaactcgc ttaagccatc atcaatatga
    49021 attaacgtca ggtgagcgtc attatgtctg gcgagctcca gggctttatt caccagtaag
    49081 gcatcttctt cattcccgga aattgccacg ccaatgtgtt tataagccat gtttaactcc
    49141 ttctaaagca actccatcaa gctaacgaac gcagtgataa ctcaaaaata atcatctcgg
    49201 cctggcatga gaaggtgaag gccgcatcaa gctcaacttg cccctccaga tccttaatct
    49261 tataggccac gctggcccct tcatctgcag aggatgcagc ggcggcaagc tctttcagtt
    49321 tggtcataaa ggactctgct tcttcgcggg tggcaaaaaa gcgcgaaaac ttactggtac
    49381 agttgtcgtt atcaatcacc gtaccgatat caatcgcaca tcctttagta ctgcatttat
    49441 ctacgacatc tttcataggg cacctctgta tattcgcctc tctgtcgatt acgacttaag
    49501 aataatgcgc cgttaaggga agaacaaaca agtaatgatc ttatggatta ccattttttt
    49561 tgatattgct taaggtgaag agttcagaag gattttccgg gcattgtcat ttgcatcgtg
    49621 gaaataacaa tcccggaaaa aaatagcagt aagagatata ttaaaggtta attacttgat
    49681 ttattgtcgg ctttatactt cacatcctga gtatctttac catatttatt ttcgccttgt
    49741 gtgccaacaa acgcaccaag atcgataagc atcatcacca gaatcaacgt cgggacaaaa
    49801 cgccctaccg cccattgcca gacacccggt aaaatcgccc agttacccgc cagtagcatc
    49861 cacgccacaa tcatcagaaa tgcccatgcg ccggaacgcc cgcgatcatg caagcgctta
    49921 acggttactg ccgctgttgg ccagagcaag cacaccaggc aaaacgccgc ggtctgaata
    49981 tcgagtaaat ttttacccgc cagtgagaaa agcaccagca tgcctgcgaa ccacaggcct
    50041 atccaaatcc agaaatcacg gcgtccaata cgccctttaa atgagaataa ccattgctgt
    50101 atggtcatgt aagttccttg atggttgtct tttccaggat tctacccttt tgacaagggc
    50161 gacagatatc gttttaatcg gagccagtca taacaaaagg tactgtcaat gaagccaggg
    50221 tgtacgctgt tttttctctt atgttctgca ttaaccgtta caacaacggc gcatgcacaa
    50281 acaccagata cggcaacgac cgcgccttat ctgctggctg gagcccctac tttcgatctc
    50341 tccatcagcc agtttcgaga agactttaac agccagaatc ccagcctgcc actgaacgaa
    50401 tttcgtgcca tcgacagcag tcccgacaaa gccaatctca ctcgtgctgc aagtaaaatt
    50461 aacgagaact tgtatgcttc tacagcgctg gagcgcggta ccttaaaaat caaaagcatt
    50521 caaatgacct ggctacccat ccaggggcca gagcaaaaag ccgcgaaagc gaaagctcag
    50581 gaatatatgg cagcggtgat ccgcacactc accccattaa tgaccaaaac acaaagccag
    50641 aaaaaactgc agtcgctact aacggcgggg aaaaacaaac gttattacac cgagacagaa
    50701 ggtgcactgc gttatgttgt cgcggacaac ggcgaaaagg ggctgacctt cgctgttgaa
    50761 ccgattaagc tggcgctatc tgaatcgctt gaaggtttga ataaatgaca aaaagcaaag
    50821 cctttgtgcc gatgaatctc tatactgttt cacagacctg ctgccctgcg gggcggccat
    50881 cttcctttat tcgcttataa gcgtggagaa ttaaaatgcg acatccttta gtgatgggta
    50941 actggaaact gaacggcagc cgccacatgg ttcacgagct ggtttctaac ctgcgtaaag
    51001 agctggcagg tgttgctggc tgtgcggttg caatcgcacc accggaaatg tacatcgata
    51061 tggcgaagcg tgaagctgaa ggcagccaca tcatgctggg tgcgcaaaac gtggacctga
    51121 acctgtccgg cgcattcacc ggtgaaacct ctgctgctat gctgaaagac atcggcgcac
    51181 agtacatcat catcggtcac tctgaacgtc gtacttacca caaagagtct gacgaactga
    51241 tcgcgaaaaa attcgcggtg ctgaaagagc agggcctgac tccggttctg tgcatcggtg
    51301 aaaccgaagc tgaaaacgaa gcgggcaaaa ctgaagaagt ttgcgcacgt cagatcgatg
    51361 cggtactgaa aactcagggt gctgcggcat tcgaaggtgc ggttatcgct tacgaacctg
    51421 tatgggcaat cggtactggc aaatctgcaa ctccggctca ggcacaggct gttcacaaat
    51481 tcatccgtga ccacatcgct aaagttgacg ctaacatcgc tgaacaagtg atcattcagt
    51541 acggcggctc tgtaaacgcg tctaacgctg cagaactgtt tgctcagcca gacatcgacg
    51601 gcgcgctggt tggcggtgcg tctctgaaag ctgacgcttt cgcagtaatc gttaaagctg
    51661 cagaagcggc taaacaggct taagtctgac aggtgccgga tttcatatcc ggcacttact
    51721 ttccttaact cttcgcctta acgcaaaatc tcacactgat gatcctgaat ttcctcggct
    51781 gaagcacggt taagcgtcag tagatttcgt tgtgtcgcca gcaatacaaa tgagttatca
    51841 ctctgccgta ccatcgccag cccgtagctt cccatatgtt cccgcgcctc aggtacttct
    51901 tctgccagca tcataaatgg gctgcgttgt accagttcgc tttccgttac ccgacgcgcc
    51961 aggtattcat gcccgcgcaa accacctggc agtggcaacc agcggctgct gatgttcgcc
    52021 agattgttat ccagctgttc gcgcacatca ggacgaatac aagagatatg aatatgaaaa
    52081 tggttttgcg tacgcccggt gcgggagttg atcgccaaag aaaccgcgcg atcgggaacc
    52141 ggctggccgt attttttgct cataaaatca cgcgcctgcc aggccaacca aaagaagttc
    52201 ggcgttgaag gatcggtcaa caaaggactt tcagtaccgt taatacgata cgttggcatc
    52261 aacagatatt gcagtgggcc attaagatct tttaaaacca cgtatccggc attgggtttg
    52321 acttccgcac atggcgaagg attttgattt tgctgctgat tgggcaaaca ttcctcaagg
    52381 acaatcttac gtaatgtatc cgactcttca ccggttaatt tccagtaacc aataccggca
    52441 gccacaacgg cgataactat catcaccaaa aaaagaagac ccgctttttt catctttttt
    52501 tccctgtacc tcaaagagat gcaagggtaa cgcaaaatcg tgacaaataa aaaacccggt
    52561 cgcgagaaac caccgggttt gataattatc ctggagaaat cagcgtttgc tgatctgatc
    52621 gaacataccg ccgttagcaa aatgctcttt ttgcgctttc gtccagccgc cgaactcttc
    52681 atcaatggtg aataacttca gctttggaaa cgcattttcg tactttttcg ccacctcagc
    52741 gtcgcgcgga cggtagtagt ttttcgcggc aatttcctga ccttctggcg agtagagata
    52801 tttcaggtag gcttccgcca cctctttagt accttttttc tcgaccactt tatcgaccac
    52861 cgacacggtt ggctctgcga ggatagactc actcggcgtg acgatttcga atttatcttt
    52921 ccccagttca ttcgctgcca gcagagcttc gttttcccag gcaatcagta catcgccaat
    52981 tccgcgctcg acaaaagtgt tagtggagcc acgcgcgcca gaatccagaa cttcgacgtt
    53041 tttatacagt gcccgaacaa aatcctgtgc ttttgcctga tcgttgttgt tgtgatgcag
    53101 cgcgtagccc caggctgcca ggtagttcca gcgcgcgcca ccagagcttt tcggattagg
    53161 cgtgatcacc gaaacacccg gtttaatcag atcgttccag tcatggatct gcttcggatt
    53221 tcccttacgt accaggaaaa caatggtgga agtgtacggt gcggagttat ccggcagacg
    53281 tttgatccac tctttatcaa tccgcccgcg ttccgcaatt gcgtccacgt cataggccag
    53341 agccagcgtg acaacatcag cttcaatacc gttgattacc gacgtcgctt gtttgcctga
    53401 accaccgtgt gactgacgaa tcaccacgtt atcgccagtt tgctgtttcc agtgggcgct
    53461 gaatgccttg ttgtactgtt cgtacaattc gcgcgttgga tcatatgaaa cgttaagaag
    53521 ctgaatatcc tttgccataa cgctggttgc cgccagcaaa aatgttaacc ctacgcccca
    53581 cttgttcatc gcccgactct cttatgttgt gttgtgatga gcaaagcgtg ccagaaggtt
    53641 aaccaaacat taaagaataa aaaaagattg gctataactt gcgggtatat gttgggggtt
    53701 aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt gcgctttatc gatgccggat
    53761 gaggcgcgaa cgccttatcc ggcctacaaa agtttgtaaa ttcaataaat tgcagaattc
    53821 acgtaggcct gataagcgaa gcgcatcagg catttttgct tctgtcatcg gtttcaggct
    53881 aaaggaatct gcctttttcc gaaatcatta atacagtttt ttcgcgcaat ccagccagtc
    53941 gcctttgaac ggacgcttca tgttttcgat agcgtcgatg atgtcgtggt gaaccagctg
    54001 ttcgttctgg atacctacgc aacgaccgcc gtaacctgcc agcagcagat cgatagcgta
    54061 agcgcccata cgggaagcca gaatacggtc gtaaggcacc ggagaaccac cgcgctggat
    54121 gtggcccagc acagttgcgc gggtttcacg accggtttct ttctcgatga aatgcgccag
    54181 ttcgtcaaca tcacacatat gttcggtaat cgccacgatc gcatgttttt tacctttcgc
    54241 gatacccgct ttgatttcgt ttaccaggtc ttcacggctg aattcaactt ccggaaccac
    54301 aacgaattca cagccaccag caatggccgc agccagcgtc agatcgccac aataacggcc
    54361 catcacttcc accacggaaa tacgctggtg agaagaagag gtgtcacgca gacggtcgat
    54421 cgcttctaca acggtgctca gcgcagtgaa gaaaccgata gtgtagtcag tgcctttgat
    54481 gtcgttgtcg atagtgcccg gcaggccgat gcacgggaag cccatttcgg tcagacgcat
    54541 tgcacccatg taggaaccgt caccgccgat aaccaccagc gcatcgatcc cacgtttttt
    54601 caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg aattccggga aacgcgcaga
    54661 accgaggaac gtaccgccac ggttgatcat gtcagaaacg ctgtaacggt ctagctgtac
    54721 catacggtct tcatacagac ccagatagcc gtcataaatg cccattactt ccagaccttc
    54781 tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc atgcctggcg catcaccgcc
    54841 gcttgtcaac acaccgattt tcttaatcat gactacctct gaactttgga atgcaaaatg
    54901 aaatctgttg ccggaagtct tcttacacat cgaagtgatc caacgaatgt gcaaatagta
    54961 taacaatcac ttcctgctga attgattcag gtcaggccaa atggcggtat tttatacaca
    55021 aaatgcgggt ctggctctct tttatactga ttatgaaagc atagaccgtt taccctccct
    55081 gggtacgacg gaacaggggt cttgatggat aatcacatcc gatcccggaa aacgccgtaa
    55141 aatagcctgc tctacctgat ccgccaccat atgtgcctga accaaaggca gagagtcttc
    55201 catttccaaa tgaatctgaa taaagcgggt cggccctgac tgccgcgtgc gaagatcgtg
    55261 agcgccgcta acacccggcc aggaagtcac gatatcaata atttcttgcc gttcctcatc
    55321 aggtaatgcg cgatccagta atgactgtac cgcctcatat cccatgcgta acgcgctata
    55381 taaaatatag atgccgattc ccaatgcaaa cagagcatcg gcgcgatgcc agccgtacca
    55441 ggacaacccc agcgcgagca gaattgcgcc gttcatcata acatcagact ggtaatgtag
    55501 catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc acccaacgct gaaacgagac
    55561 aaggataatc gtacaaatta gcgccacaat tgtcacgata accccgacgc ctggatctgt
    55621 catcggtgtt ggagatatca gatgttgaat acccgtcaaa aacaggaata gtgccgaacc
    55681 ggagataaac atactttgcg ccagcgccgc gagggactca gctttaccgt gaccaaacga
    55741 gtgattatcg tcggcaggtt gcagggaata tcgcaccacc agtaaattcg tcaacgacgc
    55801 gccgatatcc accagcgaat ccaccagcgc ggcgagaata ctcaccgacc cggtatacca
    55861 ccatgcaaaa attttaatca gcaatagcag cgaagccatc gccgtcgcag caatcgccgc
    55921 ccgactgacc agccgtccat aagattgatt cataaatact cccgctatca actgacgcta
    55981 gtataacgga agcaaatcat ctgcaatgca ttaagcagca ggcaaattga ggataaaaaa
    56041 aacccccaca tcatgtgggg gaagacaggg atggtgtcta tggcaaggaa aacagggttt
    56101 actactggga acgtgagttg ctactactca atagcttcaa cgatgaactt ttttgccatt
    56161 gcgtcacgtc gcgcaactgc tccattcgtt gttgatgttt ctcgtttaaa accgcttgct
    56221 gctccggcgt taacaggcga tacatttggt tgcggacttt tgccatctca acctgacgag
    56281 caatttgctc attcgccatt ttttctgcct gtgcgcgcac agcgttttca tcaaaatttt
    56341 ctgcggtgac aaggcgatgc attgtctcca gttcgctaac attaacagga ggctgttcgt
    56401 gccgggcctg ttgcataaga tctcgcatct gctgacgctg atgttcggtt aaacttatgc
    56461 cgtcgaacat atggctctgc gtactgcgct gcgtaagttc ttcacccgga tgccagttat
    56521 cgcctgaacc gacttcagca gcgtggctta atgaactgac tgccagcgtt gaggccatga
    56581 cggcagcggt aactatgcgc atcatttgct cccaaaatct ttctgtcgcg attcaacgat
    56641 agagagttta cgattcaggc tgcaaacatg cgtcaggggg tgtaaaacaa cgtaaagtca
    56701 tggattagcg acgtctgatg acgtaatttc tgcctcggag gtatttaaac aatgaataaa
    56761 atcctgttag ttgatgatga ccgagagctg acttccctat taaaggagct gctcgagatg
    56821 gaaggcttca acgtgattgt tgcccacgat ggggaacagg cgcttgatct tctggacgac
    56881 agcattgatt tacttttgct tgacgtaatg atgccgaaga aaaatggtat cgacacatta
    56941 aaagcacttc gccagacaca ccagacgcct gtcattatgt tgacggcgcg cggcagtgaa
    57001 cttgatcgcg ttctcggcct tgagctgggc gcagatgact atctcccgaa accgtttaat
    57061 gatcgtgagc tggtggcacg tattcgcgcg atcctgcgcc gttcgcactg gagcgagcaa
    57121 cagcaaaaca acgacaacgg ttcaccgaca ctggaagttg atgccttagt gctgaatcca
    57181 ggccgtcagg aagccagctt cgacgggcaa acgctggagt taaccggtac tgagtttacc
    57241 ctgctctatt tgctggcaca gcatctgggt caggtggttt cccgtgaaca tttaagccag
    57301 gaagtgctgg gcaaacgcct gacgcctttc gaccgcgcta tcgatatgca catttccaac
    57361 ctgcgtcgta aactgccgga tcgtaaagat ggtcacccgt ggtttaaaac cttgcgtggt
    57421 cgcggctacc tgatggtttc tgcttcatga taggcagctt aaccgcgcgc atcttcgcca
    57481 tcttctggct gacgctggcg ctggtgttga tgttggtttt gatgttaccc aagctcgatt
    57541 cacgccagat gaccgagctt ctggatagcg aacagcgtca gggtctgatg attgagcagc
    57601 atgttgaagc ggagctggcg aacgatccgc ccaacgattt aatgtggtgg cggcgtctgt
    57661 tccgggcgat tgataagtgg gcaccgccag gacagcgttt gttattggtg accaccgaag
    57721 gccgcgtgat cggcgctgaa cgcagcgaaa tgcagatcat tcgtaacttt attggtcagg
    57781 ccgataacgc cgatcatccg cagaagaaaa agtatggccg cgtggaactg gtcggtccgt
    57841 tctccgtgcg tgatggcgaa gataattacc aactttatct gattcgtccg gccagcagtt
    57901 ctcaatccga tttcattaac ttactgtttg accgcccgct attactgctg attgtcacca
    57961 tgctggtcag tacgccgctg ctgttgtggt tggcctggag tctggcaaaa ccggcgcgta
    58021 agctgaaaaa tgctgccgat gaagttgccc agggaaactt acgccagcac ccggaactgg
    58081 aagcggggcc acaggaattc cttgccgcag gtgccagttt taaccagatg gtcaccgcgc
    58141 tggagcgcat gatgacctct cagcagcgtc tgctttctga tatctctcac gagctgcgca
    58201 ccccgctgac gcgtctgcaa ctgggtacgg cgttactgcg ccgtcgtagc ggtgaaagca
    58261 aggaactgga gcgtattgaa accgaagcgc aacgtctgga cagcatgatc aacgatctgt
    58321 tggtgatgtc acgtaatcag cagaaaaacg cgctggtaag cgagacattg aaagctaacc
    58381 agttgtgggg cgaagtgctg gataacgcgg cgttcgaagc agaacagatg ggcaagtcgt
    58441 tttctgttaa cttcccgcct gggccgtggc cgctgtacgg caatccgaac gccctggaaa
    58501 gtgcgctgga aaacattgtt cgtaatgctc tgcgttattc ccatacgaag attgaagtgg
    58561 gctttgcggt agataaagac ggtatcacca ttacggtgga cgacgatggt cctggcgtta
    58621 gcccggaaga tcgcgaacag attttccgtc cgttctatcg gaccgatgaa gcgcgcgatc
    58681 gtgaatctgg cggtacaggt ttgggactgg cgattgttga aaccgccatt cagcagcatc
    58741 gtggctgggt gaaagcagaa gacagcccgc tgggcggttt acggctggtg atttggttgc
    58801 cgctgtataa gcggagttaa actccgcatt tgtaggcagg ataaggcgtt tacgccgcat
    58861 acggcatttg agcaggatgc ctgatgcgac gctgatagcg tcttatcagg cctacactcc
    58921 gagggttatt ttccccacaa ccgccgggaa aaatcttcga tcttgccgct cagtcggcgc
    58981 ttttgcatcg tccgcgtcca gcttttcgat aacccggcgg cggagagtaa acggtgatac
    59041 tgatcgtcat caaacggcat atgccatgcg atggcggcag cctcctgcac ggtcacatcg
    59101 ctgacacggg aaaccaactc cagcggcgcg tccgcagaca ctttccctgg cgctatcacg
    59161 ctatacaacc atcccacttt acccgtgttt tgcatcaact gcgctatatc gctgatatca
    59221 aaatggtaat tgagtttata gcaaggcgag cgcggctggc tgacctgaat taatgcctct
    59281 ccccagcgga aaatatcgcc aatataaaca ttactttccg tgagaccgtc ggttgagaga
    59341 ttttcgccaa acgcaggcgc aacaaacaac tccgcctgtt ccggaaattc ccgcgcccag
    59401 tagagatagt gctcacgagg ataatgacac agcgctctgt ctggcccacc gtgaaccttt
    59461 ttctccgcct gctcgtcacc ttccagcccc aggtccgtca gcatcaactc accatcaacc
    59521 tggattttgg caattgcgct gggtttgcct tcgggataag cctgaatctt gcctgtgtat
    59581 acatcaaccg gatatcgcat ctgttccctc tccacgagaa atttccccat tagaccatat
    59641 ttattttatg tcaggcgtgc tgttttcgcg gtaagtatcc aggcaggaac tcaatagcaa
    59701 accggacgaa cgttagttca tccggctgtg gggattaagg aagcaggttt atttcaccgt
    59761 acctaaaatt tcgattttcg ctgctctggc tttgacttta cgtgaccaga tagaggtaag
    59821 aattggcacc agaatcgaag tcacaatgac cgccgtcgct accagcgaag ttgctgccgg
    59881 agccatcggt ttaaacgcag gcaccatttc tgcaatcagc acaggtgtcg ctaccgcggc
    59941 ccctgcggaa ctggaagcgg caattccggc agtgccatcg ccaccgccaa tcaatttatc
    60001 ggcgataatc aacggaatac cggtcacgat aattactgcc acacccaaca ggatccccag
    60061 caaacctgtc tgggcaatta cagtcaaatc aatggtattg cctagtgcga aggcaaagaa
    60121 tggaatcagc gtctgcaccg ctttgctgaa aaattctcgt aattcagggt caaggttccc
    60181 aagggcaaag cccaccagga acggtaatac tgcgccgacg aaaacatgcg gttcaaacga
    60241 ggcaatcccg gcagtgccca gaataatcat cgtcatgagc ggcccggact ccaacgacat
    60301 caacacaaat gccccagctt cttcttttgt gccgtactgc tgcatgatgg aagcgtaaag
    60361 tccgccgttg gtcatatcca tcgccgccac cagcgccagc gttgaaagtc cggcaaagaa
    60421 tccaacttca acaccatgtt ccggaataat gcgcgaggca attgccgcaa ccacccacgc
    60481 gacggcaatt ttagttacca ccagcgtacc ggatttacgc agtaccgttc ccgtcgcgct
    60541 taattttatt gacgccccca tgcaaaaaaa ccacaccgcc agaatgggca ccgtaccggt
    60601 aatcatcccg ttggtgaatg atccaaaata tttccccgcc cccggcgaga aggtgtggca
    60661 cagtgcgcca aggaatagcg ggacgagcat catccccccc gggattttct caatcgagcg
    60721 ttttatctgc atttccatca cctgtcggca tatttttaag ggctgatgcg taattcctca
    60781 atggagcatc agtgttgatg gaaacgaaga tagacattca tttaatcaat aaaagtgatc
    60841 aaaaccacat caattgaaac gctgttttat ttttatcgga tcattgtttt tattttgaaa
    60901 ttgatcacaa aaaaaccacc gccattggcg atggttcatt ttttaagctg atatgcggcg
    60961 taaacgcctc attgcagcag acggcaaatg attatttttt cgccgcgaaa cgtgccgctg
    61021 cttcgtccca gttcaccacg ttccagaact ctttgatgta gtccggacgg cggttctgga
    61081 atttcaggta gtaagcatgt tcccacacat ccaggcccag aatcgggaag ccggaagcgc
    61141 cagaaatagc ttcacccatc agcggggagt cctggttagc agtagaaacc accgccagtt
    61201 tatcgccttt cagcaccagc catgcccagc cggaaccaaa gcgggaagct gccgcttttt
    61261 caaattctgc tttgaagtta tcaacggagc cgaagtcacg ttcgatagcc gctttcaggt
    61321 caccctgcag ggtggtgcct tttttcagac ctttccagaa caggctgtgg ttagcgtgac
    61381 cgccagcgtt gttgcgcagt acggttttct tgtctgctgg cagctggtcc agtttggtga
    61441 tcagctcttc aaccggcagg ttggcaaatt ctggcaggct ttccagcgcc gcgttggcgt
    61501 tgtttacgta ggtctgatgg tgtttggtgt ggtggatttc catggtctgc ttatcgaagt
    61561 gcggttccag ggcatcgtaa gcatacggca gggatggcag ggtatagctc atattcatct
    61621 ccagtattgt cgggcggccg attgttaatg ccgcgtaagc agttggttca ttatagttaa
    61681 ttaaatgata ttgaaaatga ttatcaatgc cgtacttttc gtaagggtat ggttttgcag
    61741 gaaaatgccc gagatgtgaa gcaaatcacc cacttaatgc cgtgattgcc agtaaatcga
    61801 caacggcggc aacaggcgaa aggttaatcg acagcacgat ttttacactc atctcgtcgg
    61861 agatgtgacg cgacgaaaaa tgatgaggat aagaagatga gtaacgcgat tacgatgggg
    61921 atattttggc atttgatcgg cgcggccagt gcagcctgtt tttacgctcc gttcaaaaaa
    61981 gtaaaaaaat ggtcatggga aaccatgtgg tcagtcggtg ggattgtttc gtggattatt
    62041 ctgccgtggg ccatcagcgc cctgttacta ccgaatttct gggcgtatta cagctcgttt
    62101 agtctctcta cgctgctgcc tgtttttctg ttcggcgcta tgtgggggat cggtaatatc
    62161 aactacggcc tgaccatgcg ttatctcggc atgtcgatgg gaattggcat cgccattggc
    62221 attacgttga ttgtcggtac gctgatgacg ccaattatca acggcaattt cgatgtgttg
    62281 attaacaccg aaggcggacg catgacgttg ctcggcgttc tggtggcgct gattggcgta
    62341 gggattgtaa ctcgagccgg gcagttgaaa gagcgcaaga tgggcattaa agccgaagag
    62401 ttcaatctga aaaaaggact ggtgctggcg gtgatgtgcg gcattttctc tgccgggatg
    62461 tcctttgcga tgaacgccgc aaaaccgatg catgaagccg ctgccgcact aggcgtcgat
    62521 ccactgtatg tcgctctgcc aagctatgtt gtcatcatgg gcggcggcgc gatcatcaac
    62581 ctcggtttct gttttattcg tctggcaaaa gtgaaggatt tatcgctaaa agccgacttc
    62641 tcgctggcaa aaccgctgat cattcacaat gtgttactct cagcactggg cgggttgatg
    62701 tggtatctgc aattcttttt ctatgcctgg ggccacgccc gcattccggc gcagtatgac
    62761 tacatcagtt ggatgctgca tatgagtttc tatgtattgt gcggcggtat cgtcgggctg
    62821 gtgctgaaag agtggaacaa tgcaggacgc cgtccggtaa cggtgttgag cctcggttgt
    62881 gtggtgatta ttgtcgccgc taacatcgtc ggcatcggca tggcgaatta atctttctgc
    62941 gaattgagat gacgccactg gctgggcgtc atcccggttt cccgggtaaa caccaccgaa
    63001 aaatagttac tatcttcaaa gccacattcg gtcgaaatat cactgattaa caggcggcta
    63061 tgctggagaa gatattgcgc atgacacact ctgacctgcc gcagatattg attgatggtc
    63121 attccagtct gctggcgaaa ttgctgacgc aaaacgcgct cactgcacga tgcctcatca
    63181 caaaatttat ccagcgcaaa gggacttttc aggctagccg ccaaccgggt aatcagctta
    63241 tccagcaacg tttcgctgga tgttggcggc aacgaatcac tggtgtaacg atggcgattc
    63301 agcaacatca ccaactgccc gaacagcaac tctgccattt cgttagcaaa cggcacatgc
    63361 tgactacttt catgctcaag ctgaccgata atctgccgcg cctgtgccat ccccatgcta
    63421 cctaagcgcc agtgtggttg ccctgcgctg gcgctaaatc ccggaatcgc cccctgccag
    63481 tcaagattca gcttcagccg ctccgggcaa taaataatat tctgcaaaac cagatcgtta
    63541 acggaagcgt aggagtgttt atcgtcagca tgaatgtaaa agagatcgcc acgggtaatg
    63601 cgataagggc gatcgttgag tacatgcaga ccattaccgc gccagacaat caccagctca
    63661 caaaaatcat gtgtatgttc agcaaagaca tcttgcggat aacggtcagc cacagcgact
    63721 gcctgctggt cgctggcaaa aaaatcatct ttgagaagtt ttaactgatg cgccaccgtg
    63781 gctacctcgg ccagagaacg aagttgatta ttcgcaatat ggcgtacaaa tacgttgaga
    63841 agattcgcgt tattgcagaa aaccatcccg tccctgacga atatcacgcg gtgaccagtt
    63901 aaactctcgg cgaaaaagcg tcgaaaagtg gttactgtcg ctgaatccac agtgataggc
    63961 gatgtcagta acgctggcct cgctgtggcg tagcagatgt cgggctttca tcagtcgcag
    64021 gcggttcagg tatcgctgag gcgtcagccc cgtttgctgc ttaagctgcc gatgtagcgt
    64081 acgcagcgaa agagaaaatt gatccgccac ggcatcccaa ttcacctcat cggcaaaatg
    64141 gtcctccagc caggccagaa gcaagttgag acgtgatgcg ctgttttcca ggttctcctg
    64201 caaactgctt ttacgcagca agagcaataa ttgcataaac aagatctcgc gactggcggt
    64261 cgagggtaaa tcattttccc cttcctgctg ttccatctgt gcaaccagct gtcgcacctg
    64321 ctgcaatacg ctgtggttaa cgcgccagtg agacggatac tgcccatcca gctcttgtgg
    64381 cagcaactga ttcagcccgg cgagaaactg aaatcgatcc ggcgagcgat acagcacatt
    64441 agtcagacac agattatcgg tatgttcata cagatgccga tcatggtcgc gcacaaaaca
    64501 gaccgtgcca ccggtgatgg tatagggctg cccattaaac acatgaatac ccgtgccatg
    64561 ttcgacaatc acaatttcat gaaaatcatg atgatgttca ggaaaatccg cctgcgggag
    64621 ccggggttct atcgccacgg acgcgttacc agacggaaaa aaatccacac tatgtaatac
    64681 ggtcatactg gcctcctgat gtcgtcaaca cggcgaaata gtaatcacga ggtcaggttc
    64741 ttaccttaaa ttttcgacgg aaaaccacgt aaaaaacgtc gatttttcaa gatacagcgt
    64801 gaattttcag gaaatgcggt gagcatcaca tcaccacaat tcagcaaatt gtgaacatca
    64861 tcacgttcat ctttccctgg ttgccaatgg cccattttcc tgtcagtaac gagaaggtcg
    64921 cgaattcagg cgctttttag actggtcgta atgaaattca gcaggatcac attatgacct
    64981 ttcgcaattg tgtcgccgtc gatctcggcg catccagtgg gcgcgtgatg ctggcgcgtt
    65041 acgagcgtga atgccgcagc ctgacgctgc gcgaaatcca tcgttttaac aatgggctgc
    65101 atagtcagaa cggctatgtc acctgggatg tggatagcct tgaaagtgcc attcgccttg
    65161 gattaaacaa ggtgtgcgag gaagggattc gtatcgatag cattgggatt gatacctggg
    65221 gcgtggactt tgtgctgctc gaccaacagg gtcagcgtgt gggcctgccc gttgcttatc
    65281 gcgatagccg caccaatggc ctaatggcgc aggcacaaca acaactcggc aaacgcgata
    65341 tttatcaacg tagcggcatc cagtttctgc ccttcaatac gctttatcag ttgcgtgcgc
    65401 tgacggagca acaacctgaa cttattccac acattgctca cgctctgctg atgccggatt
    65461 acttcagtta tcgcctgacc ggcaagatga actgggagta caccaatgcc acaaccacgc
    65521 aactggtcaa tatcaatagc gacgactggg acgagtcgct actggcgtgg agcggggcca
    65581 acaaagcctg gtttggtcgc ccgacgcatc cgggtaatgt cataggtcac tggatttgcc
    65641 cgcagggtaa tgagattcca gtggtcgccg ttgccagcca tgataccgcc agcgcggtta
    65701 tcgcctcgcc gttaaacggt tcacgtgctg cttatctctc ctctggcacc tggtcattga
    65761 tgggcttcga aagccagacg ccatttacca atgacacggc actggcagcc aacatcacca
    65821 atgaaggcgg ggcggaaggt cgctatcggg tgctgaaaaa tattatgggc ttatggctgc
    65881 ttcagcgagt gcttcaggag cggcaaatca acgatcttcc ggcgcttatc gccgcgacac
    65941 aggcacttcc ggcttgccgc ttcattatca atcccaatga cgatcgcttt attaatcctg
    66001 agacgatgtg cagcgaaatt caggctgcgt gtcgggaaat ggcgcaaccg atcccggaaa
    66061 gtgatgctga actggcgcgc tgtattttcg acagtctggc gttgctgtat gccgatgtgt
    66121 tgcatgagct ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg
    66181 gctgccagaa cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg
    66241 ccgggcctgt tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg
    66301 aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct
    66361 ttacccctaa tcctgacagt gaaattgccc actatgtggc gcagcttcac tctacacgac
    66421 agacaaagga gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca
    66481 gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc
    66541 cgtttcaatg cactgctggc agggtgatga tgtttccggt tttgaaaacc cggaaggttc
    66601 gctgaccgga gggattcagg ctactggcaa ttatccgggc aaagcgcgta atgccagtga
    66661 gctacgtgcc gatctggaac aggctatgcg gctgattccg gggccaaaac ggcttaattt
    66721 acatgccatc tatctggaat cagacacgcc agtctcgcgc gaccagatca aacctgagca
    66781 cttcaaaaac tgggttgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc
    66841 ctgtttctcg catccgctaa gcgccgatgg ctttacgctt tcccatgccg acgacagcat
    66901 tcgccagttc tggattgatc actgcaaggc cagccgccgc gtttcggcct attttggtga
    66961 gcaactcggc acaccgtcgg tgatgaacat ctggatcccg gatggcatga aagatatcac
    67021 cgttgaccgt ctcgctccgc gccagcgtct gctggcagct ctggatgagg tgatcagcga
    67081 gaagctggat ccggcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc
    67141 agagagctac acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac
    67201 tgcgctgtgc ctggacgccg ggcatttcca cccgactgaa gtgatttccg acaagatttc
    67261 cgccgccatg ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga
    67321 cagcgatcac gtagtgctgc tggatgatga aacccaggcg attgccagtg agattgttcg
    67381 tcacgatctg ttcgaccggg tgcatatcgg cctcgacttc tttgatgcct ctatcaaccg
    67441 tattgctgcg tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct
    67501 ggaacctacc gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc
    67561 actgctggaa gagcagaaat cgttgccgtg gcaggcggta tgggaaatgt attgccaacg
    67621 tcacgatacc ccagcaggta gcgaatggct ggagagcgtg cgggcttatg agaaagaaat
    67681 tttgagtcgc cgcgggtaaa cactgccgga tgcggcacaa gtgccttatc cggcctacag
    67741 gtcggcaaca gttgtaggcc tgataagacg caacagcgtc gcatcaggca ttgattgccg
    67801 gatgcggcac aagtgcctta tccggcctac aggtcggcaa tagttgtagg cctgataaga
    67861 cgcgacagcg tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatctggcct
    67921 ac
//
LOCUS       DAPGRN010000011        66287 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPGRN010000011 DAPGRN010000000
VERSION     DAPGRN010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.011"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(208..284)
                     /locus_tag="RHW33_000794"
     tRNA            complement(208..284)
                     /locus_tag="RHW33_000794"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Arg,seq:acg)
     gene            complement(288..380)
                     /locus_tag="RHW33_000795"
     tRNA            complement(288..380)
                     /locus_tag="RHW33_000795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(344..346),aa:Ser,seq:gct)
     gene            complement(696..881)
                     /gene="csrA"
                     /locus_tag="RHW33_000796"
     CDS             complement(696..881)
                     /gene="csrA"
                     /locus_tag="RHW33_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV1644338.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1116..3746)
                     /gene="alaS"
                     /locus_tag="RHW33_000797"
     CDS             complement(1116..3746)
                     /gene="alaS"
                     /locus_tag="RHW33_000797"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV1644339.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFVVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3874..4374)
                     /gene="recX"
                     /locus_tag="RHW33_000798"
     CDS             complement(3874..4374)
                     /gene="recX"
                     /locus_tag="RHW33_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV1644340.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4617..5678)
                     /gene="recA"
                     /locus_tag="RHW33_000799"
     CDS             complement(4617..5678)
                     /gene="recA"
                     /locus_tag="RHW33_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV1644341.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5758..6255)
                     /gene="pncC"
                     /locus_tag="RHW33_000800"
     CDS             complement(5758..6255)
                     /gene="pncC"
                     /locus_tag="RHW33_000800"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV1644342.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6400..7485)
                     /gene="mltB"
                     /locus_tag="RHW33_000801"
     CDS             complement(6400..7485)
                     /gene="mltB"
                     /locus_tag="RHW33_000801"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV1644343.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7741..8304
                     /gene="srlA"
                     /locus_tag="RHW33_000802"
                     /gene_synonym="gutA"
     CDS             7741..8304
                     /gene="srlA"
                     /locus_tag="RHW33_000802"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV1644344.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8301..9260
                     /gene="srlE"
                     /locus_tag="RHW33_000803"
                     /gene_synonym="gutE"
     CDS             8301..9260
                     /gene="srlE"
                     /locus_tag="RHW33_000803"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV1644345.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGIAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9271..9642
                     /gene="srlB"
                     /locus_tag="RHW33_000804"
                     /gene_synonym="gutB"
     CDS             9271..9642
                     /gene="srlB"
                     /locus_tag="RHW33_000804"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV1644346.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9646..10425
                     /gene="srlD"
                     /locus_tag="RHW33_000805"
     CDS             9646..10425
                     /gene="srlD"
                     /locus_tag="RHW33_000805"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV1644347.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINITGGQVMF"
     gene            10531..10890
                     /gene="gutM"
                     /locus_tag="RHW33_000806"
     CDS             10531..10890
                     /gene="gutM"
                     /locus_tag="RHW33_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV1644348.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIADTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10957..11730
                     /gene="srlR"
                     /locus_tag="RHW33_000807"
                     /gene_synonym="gutR"
     CDS             10957..11730
                     /gene="srlR"
                     /locus_tag="RHW33_000807"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV1644349.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALAELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDADIDPAFRQALEEKGIDVIITGESNE"
     gene            11723..12688
                     /gene="gutQ"
                     /locus_tag="RHW33_000808"
     CDS             11723..12688
                     /gene="gutQ"
                     /locus_tag="RHW33_000808"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV1644350.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQNRAIDAKEILMKRKITAAPVVDENGKLVGAINLQDFYQAGII"
     gene            complement(12685..14199)
                     /gene="norR"
                     /locus_tag="RHW33_000809"
     CDS             complement(12685..14199)
                     /gene="norR"
                     /locus_tag="RHW33_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV1644351.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPDDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14386..15825
                     /gene="norV"
                     /locus_tag="RHW33_000810"
     CDS             14386..15825
                     /gene="norV"
                     /locus_tag="RHW33_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV1644352.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15822..16955
                     /gene="norW"
                     /locus_tag="RHW33_000811"
     CDS             15822..16955
                     /gene="norW"
                     /locus_tag="RHW33_000811"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV1644353.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLGHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKMVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGIQA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPM"
     gene            complement(17084..19336)
                     /gene="hypF"
                     /locus_tag="RHW33_000812"
     CDS             complement(17084..19336)
                     /gene="hypF"
                     /locus_tag="RHW33_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV1644354.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPEICDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALEDLQGIADGFLIHNRDIVQRMDDSVVRESGEMMRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGE
                     NGALWGGECLLVNYRECEHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19489..20016)
                     /gene="hydN"
                     /locus_tag="RHW33_000813"
     CDS             complement(19489..20016)
                     /gene="hydN"
                     /locus_tag="RHW33_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV1644355.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20166..21176)
                     /gene="ascG"
                     /locus_tag="RHW33_000814"
     CDS             complement(20166..21176)
                     /gene="ascG"
                     /locus_tag="RHW33_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV1644356.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHKRGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21436..22893
                     /gene="ascF"
                     /locus_tag="RHW33_000815"
     CDS             21436..22893
                     /gene="ascF"
                     /locus_tag="RHW33_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV1644357.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22902..24326
                     /gene="ascB"
                     /locus_tag="RHW33_000816"
     CDS             22902..24326
                     /gene="ascB"
                     /locus_tag="RHW33_000816"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV1644358.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFKAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAASGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24441..24860)
                     /locus_tag="RHW33_000817"
     CDS             complement(24441..24860)
                     /locus_tag="RHW33_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1644359.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24886..25071)
                     /locus_tag="RHW33_000818"
     CDS             complement(24886..25071)
                     /locus_tag="RHW33_000818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644360.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGV"
     gene            complement(25096..25563)
                     /gene="hycI"
                     /locus_tag="RHW33_000819"
     CDS             complement(25096..25563)
                     /gene="hycI"
                     /locus_tag="RHW33_000819"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV1644361.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEE"
     gene            complement(25556..25966)
                     /gene="hycH"
                     /locus_tag="RHW33_000820"
     CDS             complement(25556..25966)
                     /gene="hycH"
                     /locus_tag="RHW33_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV1644362.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25963..26730)
                     /gene="hycG"
                     /locus_tag="RHW33_000821"
     CDS             complement(25963..26730)
                     /gene="hycG"
                     /locus_tag="RHW33_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV1644363.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26730..27272)
                     /gene="hycF"
                     /locus_tag="RHW33_000822"
     CDS             complement(26730..27272)
                     /gene="hycF"
                     /locus_tag="RHW33_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV1644364.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27282..28991)
                     /gene="hycE"
                     /locus_tag="RHW33_000823"
     CDS             complement(27282..28991)
                     /gene="hycE"
                     /locus_tag="RHW33_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV1644365.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(29009..29932)
                     /gene="hycD"
                     /locus_tag="RHW33_000824"
     CDS             complement(29009..29932)
                     /gene="hycD"
                     /locus_tag="RHW33_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV1644366.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29935..31761)
                     /gene="hycC"
                     /locus_tag="RHW33_000825"
     CDS             complement(29935..31761)
                     /gene="hycC"
                     /locus_tag="RHW33_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV1644367.1"
                     /translation="MSAISLINSGVAWFVAAAILAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVDLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31758..32369)
                     /gene="hycB"
                     /locus_tag="RHW33_000826"
     CDS             complement(31758..32369)
                     /gene="hycB"
                     /locus_tag="RHW33_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV1644368.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32494..32955)
                     /gene="hycA"
                     /locus_tag="RHW33_000827"
     CDS             complement(32494..32955)
                     /gene="hycA"
                     /locus_tag="RHW33_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV1644369.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33167..33517
                     /gene="hypA"
                     /locus_tag="RHW33_000828"
     CDS             33167..33517
                     /gene="hypA"
                     /locus_tag="RHW33_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV1644370.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33521..34393
                     /gene="hypB"
                     /locus_tag="RHW33_000829"
     CDS             33521..34393
                     /gene="hypB"
                     /locus_tag="RHW33_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV1644371.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQNEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34384..34656
                     /gene="hypC"
                     /locus_tag="RHW33_000830"
     CDS             34384..34656
                     /gene="hypC"
                     /locus_tag="RHW33_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV1644372.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34656..35777
                     /gene="hypD"
                     /locus_tag="RHW33_000831"
     CDS             34656..35777
                     /gene="hypD"
                     /locus_tag="RHW33_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV1644373.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35774..36784
                     /gene="hypE"
                     /locus_tag="RHW33_000832"
     CDS             35774..36784
                     /gene="hypE"
                     /locus_tag="RHW33_000832"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV1644374.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLIAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36858..38936
                     /gene="flhA"
                     /locus_tag="RHW33_000833"
     CDS             36858..38936
                     /gene="flhA"
                     /locus_tag="RHW33_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV1644375.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(38973..39326)
                     /locus_tag="RHW33_000834"
     CDS             complement(38973..39326)
                     /locus_tag="RHW33_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV1644376.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTARHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(39358..39537)
                     /locus_tag="RHW33_000835"
     CDS             complement(39358..39537)
                     /locus_tag="RHW33_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644377.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSVG
                     EPFFFRVIVPFKIKL"
     gene            39613..42174
                     /gene="mutS"
                     /locus_tag="RHW33_000836"
     CDS             39613..42174
                     /gene="mutS"
                     /locus_tag="RHW33_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV1644378.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIAPGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42280..42936
                     /gene="pphB"
                     /locus_tag="RHW33_000837"
     CDS             42280..42936
                     /gene="pphB"
                     /locus_tag="RHW33_000837"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV1644379.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISIGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(42977..43213)
                     /locus_tag="RHW33_000838"
     CDS             complement(42977..43213)
                     /locus_tag="RHW33_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644380.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(43224..44650)
                     /locus_tag="RHW33_000839"
                     /pseudo
     CDS             complement(43224..44650)
                     /locus_tag="RHW33_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(44650..45243)
                     /locus_tag="RHW33_000840"
     CDS             complement(44650..45243)
                     /locus_tag="RHW33_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV1644381.1"
                     /translation="MRLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFCHNPADQAATISSGSFRTDGMIVIPCSMKTLAGIRAGYAEGL
                     VGRAADVILKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETIDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQVRNFSQENE"
     gene            45390..45797
                     /locus_tag="RHW33_000841"
     CDS             45390..45797
                     /locus_tag="RHW33_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV1644382.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDAADKRRRFVWLTAEGEKILAAA
                     IPIGDRVDEEFLGRLSVEEQELFMQLVRKMMST"
     gene            complement(45917..46909)
                     /gene="rpoS"
                     /locus_tag="RHW33_000842"
     CDS             complement(45917..46909)
                     /gene="rpoS"
                     /locus_tag="RHW33_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV1644383.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(46972..48111)
                     /gene="nlpD"
                     /locus_tag="RHW33_000843"
     CDS             complement(46972..48111)
                     /gene="nlpD"
                     /locus_tag="RHW33_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV1644384.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLIKPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(48251..48877)
                     /gene="pcm"
                     /locus_tag="RHW33_000844"
     CDS             complement(48251..48877)
                     /gene="pcm"
                     /locus_tag="RHW33_000844"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV1644385.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(48871..49632)
                     /gene="surE"
                     /locus_tag="RHW33_000845"
     CDS             complement(48871..49632)
                     /gene="surE"
                     /locus_tag="RHW33_000845"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV1644386.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(49613..50662)
                     /gene="truD"
                     /locus_tag="RHW33_000846"
     CDS             complement(49613..50662)
                     /gene="truD"
                     /locus_tag="RHW33_000846"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV1644387.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(50659..51138)
                     /gene="ispF"
                     /locus_tag="RHW33_000847"
     CDS             complement(50659..51138)
                     /gene="ispF"
                     /locus_tag="RHW33_000847"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV1644388.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(51138..51848)
                     /gene="ispD"
                     /locus_tag="RHW33_000848"
     CDS             complement(51138..51848)
                     /gene="ispD"
                     /locus_tag="RHW33_000848"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV1644389.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(51867..52178)
                     /gene="ftsB"
                     /locus_tag="RHW33_000849"
     CDS             complement(51867..52178)
                     /gene="ftsB"
                     /locus_tag="RHW33_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV1644390.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(52372..52695)
                     /locus_tag="RHW33_000850"
     CDS             complement(52372..52695)
                     /locus_tag="RHW33_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV1644391.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(52745..53350)
                     /gene="cysC"
                     /locus_tag="RHW33_000851"
     CDS             complement(52745..53350)
                     /gene="cysC"
                     /locus_tag="RHW33_000851"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV1644392.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(53350..54777)
                     /gene="cysN"
                     /locus_tag="RHW33_000852"
     CDS             complement(53350..54777)
                     /gene="cysN"
                     /locus_tag="RHW33_000852"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV1644393.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(54779..55687)
                     /gene="cysD"
                     /locus_tag="RHW33_000853"
     CDS             complement(54779..55687)
                     /gene="cysD"
                     /locus_tag="RHW33_000853"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV1644394.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            55939..56976
                     /gene="iap"
                     /locus_tag="RHW33_000854"
     CDS             55939..56976
                     /gene="iap"
                     /locus_tag="RHW33_000854"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV1644395.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   57058..57574
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=57058..57086
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(57671..57964)
                     /gene="cas2"
                     /locus_tag="RHW33_000855"
     CDS             complement(57671..57964)
                     /gene="cas2"
                     /locus_tag="RHW33_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV1644396.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(57961..58884)
                     /gene="cas1e"
                     /locus_tag="RHW33_000856"
     CDS             complement(57961..58884)
                     /gene="cas1e"
                     /locus_tag="RHW33_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HDV1644397.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTRIRTHI
                     PVGSVAYIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVDQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQLAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            complement(58881..59531)
                     /gene="cas6e"
                     /locus_tag="RHW33_000857"
     CDS             complement(58881..59531)
                     /gene="cas6e"
                     /locus_tag="RHW33_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HDV1644398.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESETFTIECRSFVPELRTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQMRGQAEGSDVWLHQQQAALDWLAVQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(59513..60259)
                     /gene="cas5e"
                     /locus_tag="RHW33_000858"
     CDS             complement(59513..60259)
                     /gene="cas5e"
                     /locus_tag="RHW33_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HDV1644399.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQGGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNASDVLRNAYHQYQEHFHDLKIALPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(60270..61325)
                     /gene="cas7e"
                     /locus_tag="RHW33_000859"
     CDS             complement(60270..61325)
                     /gene="cas7e"
                     /locus_tag="RHW33_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HDV1644400.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQLITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(61340..61876)
                     /gene="casB"
                     /locus_tag="RHW33_000860"
     CDS             complement(61340..61876)
                     /gene="casB"
                     /locus_tag="RHW33_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HDV1644401.1"
                     /translation="MSIVKEEHKATLRKWHEELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSEGRFTRL
                     SAVKTPDDLLRQLRRAVKLLNGSVNLISLAEDIFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQN"
     gene            complement(61873..63435)
                     /gene="casA"
                     /locus_tag="RHW33_000861"
     CDS             complement(61873..63435)
                     /gene="casA"
                     /locus_tag="RHW33_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HDV1644402.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLLLEHAFQFGPDSPSFM
                     QDFEELRVKVSSIASLLPDTPGKQTKERNKDHFIKRGTIQHICLHCVPLALFSIQLNA
                     PKGGRGYYPGLRGGGPLTTLIELLEYQGNPQPPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTCELDGAVITDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICDSQSGA
                     LLSLMALKNNGVQYVMWRHPLTPYRMPLKEGGEFYSVKPQPGGLIWRDWLGLIEEGKS
                     KNNTELPAQVVKLFNASNLKQTKIGLWGFGYDFEDMKVRCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDPDELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQNA
                     KAAREKKQEAAE"
     gene            complement(63525..66224)
                     /locus_tag="RHW33_000862"
     CDS             complement(63525..66224)
                     /locus_tag="RHW33_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDV1644403.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRDEKEGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLHP
                     QLKVPEGARKNYEKISHSTLGYWLWHHYLSECEELLPASSLSTRKLKRVIAMWMPMTT
                     GHHGRPPDRIDELDNFLPEDKVAARDFLLEIKALCPLIEIPTFWDNDEGVELLKQLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKDYWQKTLVQAQNALTVFPPKAETAPFTGV
                     NTLFPFIEHPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAARKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLTHSARTLMDRFNESLWSGDLV
                     GSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQ
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQESIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKN
                     CNLKRSSQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFMLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYASGAHAWEMSVARVRRSWWKKHRDEFSLL
                     EGEAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVD"
BASE COUNT        15674 a        18171 c        16981 g        15461 t
ORIGIN      
        1 acggatgcat cgggaaactt actttactgc agattttttg ataccgctac taaagccgta
       61 tcaagtaaga gatggtgcat ccgggaggat tactcgcctt gcggctcgcc cttcgggccg
      121 ttgctaaagc aacgttatcc tccctggtgc ctgcggttag taccgccacc tcttccatac
      181 cttcatcgct gaaagttacg gagaatatgg tgcatccggg aggattcgaa cctccgaccg
      241 ctcggttcgt agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg
      301 gggattcgaa ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc
      361 agcctctcgg ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc
      421 tcatcgtcgc tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc
      481 ccacactttt atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag
      541 tgttttatca acagaaaaat ggaggtctga cagatagcag taatgtaaaa aaatggagac
      601 ttaagttgaa tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg
      661 gtgcgtctca ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt
      721 tcagcctgga tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt
      781 acgccaatac gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca
      841 atcatgaggg tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat
      901 taaaagagtc gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag
      961 caatgacaaa cacattacat ctaagcagtc atggcattac attctgttaa acctaagttt
     1021 agccgatata cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc
     1081 gtgaagcgag tccacggcat tgccttacgc ttatattatt gcaatttcgc gctgacccag
     1141 cctttcacac tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc
     1201 atgtcaggac gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc
     1261 cctgctttca cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct
     1321 tcggctaccg ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc
     1381 atggtacgca acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca
     1441 ttaacatcaa ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta
     1501 agctgttgta gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatca
     1561 gccagattat tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct
     1621 gcatgaacgg tggcgatagc accttctccg gttaccgctt cgatacgacg aacgcctgca
     1681 gcagtacccg attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga
     1741 gtaccgccac acagctcggt ggagaaatcg cccatgctca gcacgcgtac gcgctcatca
     1801 tacttctcgc cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg
     1861 ttggtttcga tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga
     1921 atctcttctg gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta
     1981 accagtgaac ctttctgcga tacatgagta cccagaacct ggcgcagcgc agcatgcatc
     2041 aggtgcgttg cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc
     2101 tgcacagcgt cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc
     2161 tggccgtatt tctgcgtatc ttccaccacg aaggagaagt tagcgccttt cagttcgcct
     2221 ttatcaccaa cctgaccgcc ggattccgca tagaatggcg tttgatccag cacgaccaca
     2281 gcttcctggc ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact
     2341 ttgccgttca gttccagatg gtcatagcct ttaaattcag atgcactgtc aacacggatc
     2401 attgcgttgt aatcggcacc aaagccgctg gcttcgcgcg cccgacgacg ctgctcttcc
     2461 attgctgctt caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc
     2521 gtcaggtcaa ccgggaagcc ataggtgtcg tacagacgga aagcagtttc accatccagc
     2581 gtatcaccag aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta
     2641 cgagcaaact gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc
     2701 aggtcttcac ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag
     2761 gtttctttcg cgccgagcat attaccgtga cgcactgcgc gacgaatgat acgacgcagt
     2821 acataaccac ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa
     2881 cgaatgtggt cagcgattac gcgcagcgat ttattgctca gatcggttgc gccagtgact
     2941 ttcgctaccg cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt
     3001 tgcagcaccg cagcaatacg ctccagaccc ataccggtat ctacagacgg tttcggcagc
     3061 ggttccatcg tgccatcggc ctggcggttg aactgcatga agacgatgtt ccagatctca
     3121 atgtagcggt cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg
     3181 tgatcgtaga agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag
     3241 ttgtcagatg cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg
     3301 atccctactt ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc
     3361 cacagacgct ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac
     3421 tgaatggcat cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag
     3481 gtatggtgac gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc
     3541 acgcagcgtt gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca
     3601 tccttgaact ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc
     3661 agggagctgc tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga
     3721 cggatctcag cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc
     3781 accagctcca gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt
     3841 tttcttgacg gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc
     3901 ctggatatct tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc
     3961 tgaaaagaca gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc
     4021 gcaccagtcg atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt
     4081 ctgattcagt tcctggcgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat
     4141 aaagcgcgca acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac
     4201 gcgctcgtaa tcttctgccg tagcatcaat ctcttctggg ccatttttgc ccataatcgg
     4261 tgccgcgagt ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac
     4321 cgcacgatcc aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata
     4381 tccttacaac ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca
     4441 gatttaatag tcaatgagtg tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga
     4501 ggtgagagta atttttcacg ccagtgctgg cctgttcccc tcaccctaac cctctcccca
     4561 aaggggcgag gggaccgtcc gtgcacacat tatctattat catcaataac gaacgattaa
     4621 aaatcttcgt tagtttctgc tacgccttcg ctatcatcta cagagaaatc cggcgttgag
     4681 ttcgggttgc tcagcagcaa ctcacgtact ttcttctcga tctctttcgc ggtttccggg
     4741 ttatctttca gccaggcagt tgcattcgct ttaccctgac cgatcttctc acctttgtag
     4801 ctgtaccacg cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc aaccagttcg
     4861 ccgtagaagt tgataccttc gccgtagagg atctggaatt cagcctgttt aaacggcgca
     4921 gcgattttgt tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct
     4981 ttcaccgcgc cgatacgacg gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta
     5041 ccaccggtag tggtttccgg gttaccgaac atcacaccaa ttttcatacg gatctggttg
     5101 atgaagatca gcagcgtgtt ggactgcttc aggttacccg ccagcttacg catcgcctgg
     5161 ctcatcatac gtgccgcaag gcccatgtga gagtcgccga tttcgccttc gatttccgct
     5221 ttcggcgtca gtgccgccac ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc
     5281 agagcgtcac agatttccag tgcctgctcg ccggtgtccg gctgggagca cagcaggttg
     5341 tcgatatcga cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc agcatcgata
     5401 aacgcacagg ttttaccttc acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt
     5461 ttaccggaag attccggtcc gtagatttcg acgatacggc ccatcggcag accacctgcc
     5521 ccaagcgcga tatccagtga aagcgaaccg gtagagatgg tttccacatc catggaacgg
     5581 tcttcaccca ggcgcatgat ggagccttta ccaaattgtt tctcaatctg gcccagtgct
     5641 gccgccaacg ctttctgttt gttttcgtcg atagccattt ttactcctgt catgccgggt
     5701 aataccggat agtcaatacg ttctgttgaa gcaattatac tgtatgctca tacagtatca
     5761 agtgttttgt agaaattgtt gccacaaggt ctgcaatgca tacgcagtag cctgacggcg
     5821 caccgcatca cggtcgccgc tgaagcattc ccgccgggta atgccttcac cgcgggcagt
     5881 ggcaaaagca aaccagacgg tgccgacagg cttctcttca ctgccgccat ccggcccggc
     5941 gataccacta atagacacgg cgtaatcagc acgagccgct ttcagtgcgc ctatcgccat
     6001 ttccaccacg acgggttcgc tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc
     6061 gatcatttgc gctttggctt cgttactgta ggtgacaaat ccgcgttcaa accaggcgga
     6121 actgccggca atatcggtaa tcactttcgc tacccaaccg ccggtacaag actcagcagt
     6181 tgttacggtt gcgccacggg ctttcagcgc ctgcccaacc tgttcgctta actgcatcag
     6241 ttcactgtca gtcatcacaa cctctgtcag tcaaaaattt atgccggaca agatagcact
     6301 ttcgtcagga agatggtgtt ggcgttgcaa ttttacgagg aggattcaga aaaaagctga
     6361 ttagccagag ggaagctcac gcccccctct tgcaaatagt tactgcacgc gcgccagcgc
     6421 cacggcttgt cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt tataacgggt
     6481 aatggtgtag aagttcggca gaccgtacca gtactggtaa ccggtaccga catccagacg
     6541 cagcaggctg gcttgttgat ggttgcccag cggctgctgt ggcgttaaac ctgccgcggc
     6601 aagctgcgaa atgctgtact tggttttgaa gccatttggc aagcctggag cctgaccgtt
     6661 tgccattacc gcgacctgat cgcctttcac ccaaccgtgc gctttgaaat agttcgccac
     6721 gctaccgatc gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac
     6781 cgcatattgt ttgtaagacg acggcataaa ctgtccgtag cccatcgccc cggcaaagga
     6841 acctttcaga ttgagcggat cgtcctgctc gtcgcgcgcc atcagcagga aggtttccag
     6901 ctcgccagag aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc
     6961 gaggatgcga gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac
     7021 gataatttcc ggcggtacgc catacacctg ccacgcgcga ttcaacgcat cttcatactg
     7081 attccagaaa accacaccgt tctgcacatt gtccggcgta ataaattttt tgcgataacg
     7141 cagccatgcg ccgttcggac ctgatggcgg tttcaccgat gtcgttggtg cctggttatc
     7201 catcagccgc agtaccgaat ccagacgttt cgcctgggag agaatttcct gcaactgctg
     7261 acgatcgaaa ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcgggttatt
     7321 ggcgaagtca ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga
     7381 cggcgttccg gtggtcgtat cagtctcagt aggttttggc ttgctgctac aggcggcaag
     7441 caacacaaaa aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa
     7501 cagattcaac caggggcaag tatggtaaag catcacgccc cgcacaagga agcggtagtc
     7561 actgcccgat acggacttta cataactcaa ctcatccccc tcgctatcct tttattaaaa
     7621 ctttcaaatt aaaatattta tctttcattt tgcgatcaaa ataacacttt taaatctttc
     7681 aatctgatta gattaggttg ccgtttggta ataaaacaat aaatcctgaa ggagagaaca
     7741 atgatagaaa ccattactca tggtgcagag tggtttatcg ggctgttcca aaagggcgga
     7801 gaggtgttta ccgggatggt gaccggaatt cttccgctgt tgattagcct gctggttatc
     7861 atgaacgcac tgattaattt tatcggtcag catcgaattg aacgttttgc tcaacgttgc
     7921 gccggtaacc ctgtttcccg ttacctgctg ctaccgtgca ttggcacgtt tgtcttttgc
     7981 aatccgatga ccttaagcct gggtcgcttt atgccggaaa agtacaaacc cagctactac
     8041 gcggcggcct cttatagctg ccactcaatg aatggcctct ttccccatat caaccctggc
     8101 gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc
     8161 ccactggcgg tgagctatct gctggttggt ctggtcacca acttcttccg cggctgggtg
     8221 accgatctga ccaccgctat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt
     8281 catctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg ggtggctggg
     8341 gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc accgccggta
     8401 cccggcctgc gattgtcgac aaactggcac agcttactgg ctggcaggct attgacggat
     8461 ttaaagaagg tgaacccgcg gaggcagaaa ttggcattgc ggtaatcgac tgtggcggca
     8521 cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg
     8581 gcaagtccgg tccactggcg cagtacattg tggaagatat ttatgtctct ggtgtaaaag
     8641 aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgta ggccgtgact
     8701 atgacaccag caagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg
     8761 gtatgggttc tgccgtagcc gtgttgtttc aatctggtcg tgacaccatc gacactgtat
     8821 taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt
     8881 ctggccttgg tgactggatt gcccacggtc ttgctccgct ggcgagccat ccactgggtc
     8941 tggtgatgct ggcgctcatc tgctccttcc cgctgctttc acctttcctc ggcccaggcg
     9001 cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc
     9061 cgccgcatct ggctttacct gcgctgtttg ccatcaacgc gcaggcggcc tgcgacttca
     9121 tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc ggtgtccctt
     9181 ctgttctggt gagccgcttt ttaaccggcg caccgactgt actgatcgcc tggtttgtct
     9241 ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc
     9301 cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa
     9361 ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt
     9421 gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt
     9481 ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt
     9541 ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtccc cgacgatatc
     9601 gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc
     9661 gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct ggctgccgag
     9721 gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa
     9781 attaacgccg aatatggtga aagtatggcg tacggttttg gtgctgacgc cactagcgag
     9841 caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg
     9901 gtctacagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt
     9961 gaccgttcac tacaagtgaa tctggtgggt tatttcctgt gcgcgcgtga attttcgcgt
    10021 ttgatgatcc gcgatgggat tcagggacgc attattcaga tcaactcgaa atccggcaaa
    10081 gtgggcagca aacacaactc tggttatagc gccgcgaaat ttggtggcgt agggctgact
    10141 caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact aatgctcggt
    10201 aacctgctga aatcgccgat gttccagtcg ctgttgccac aatacgcgac caagctgggt
    10261 atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc
    10321 gattatcagg atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc gtcgtactgc
    10381 accggacaat cgatcaatat caccggcggt caggtgatgt tctgatcaac agcggagatc
    10441 cgttaaggat ctccgtgaga ctaatagaat gcctgatgcg ctacgcttat caggcctaca
    10501 ggacttccgc cactacatta aggaaaagtt atggtatccg cactcatcac tgtcgccgtt
    10561 atcgcctggt gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc
    10621 ttcgacacac tatgccagca agggcgggtt ggcgtgggcc gttccagcgg gcgctttaaa
    10681 ccgcgggtcg tggtcgccat cgccctggac gatcagcagc gcatcgccga caccttgttt
    10741 atgaaaggac tgaccgtctt cgcccgaccg caaaaaattc ccgcaattac cggcatgcat
    10801 gcgggtgatt tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta
    10861 tcattggcgc ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag
    10921 ttatcatttt gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc
    10981 gccattctgg agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac
    11041 tttgacacca caggcacaac tattcgcaaa gatctggtca ttctggaaca tgccggaacc
    11101 gtcattcgta cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat
    11161 cataaaacac tcatcaacac ccacaagaaa gagctgatag cagaagctgc cgttggtttt
    11221 attcatgatg gcgattcgat cattcttgat gctggcagta ccgttttgca gatggtgcct
    11281 ctgctctcgc gctttaataa catcacggtg atgaccaaca gcctgcatat cgtcaatgcg
    11341 ctagccgaac tggataacga acaaactatc ctgatgccag gcggaacgtt tcgtaaaaaa
    11401 tcggcctcat ttcacgggca gctggcagag aatgccttcg agcatttcac cttcgacaaa
    11461 ttattcatgg gtaccgacgg catcgatctc aatgcgggcg taaccacctt caacgaggtc
    11521 tataccgtca gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca
    11581 tcaaagtttg gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg
    11641 attaccgacg cagatatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat
    11701 gtgatcataa ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt
    11761 aatgctggaa ttgcaggaag caagccgttt accggaacgt ctgggcgatg attttgttcg
    11821 cgccgccaat atcatcctgc actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc
    11881 gggccacatt ggtaagaaaa tcgccgcaac gcttgccagt accggcacac cggctttttt
    11941 tgtccatccg gcagaagcgc tgcacggcga tctggggatg atcgaaagcc gcgatgtgat
    12001 gctgtttatc tcttactccg gtggggcgaa ggaactggat ctgattattc cgcgtctgga
    12061 agacaaatct atcgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc
    12121 ggcgaaagcg gtgctggata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc
    12181 gacctccagc accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca
    12241 ggcgcgcgga tttaatgaag aagattttgc ccgctcccac ccagccgggg cactgggcgc
    12301 tcgcttgctg aataaagtgc atcatctgat gcgtcgtgat gacgctatcc cacaggtagc
    12361 gttaaccgcc agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc tggggctggt
    12421 ggcggtatgt gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg
    12481 ctggctggtt ggcggcggcg cactcaccac gccagttaat gaagcgatga cgacaggcgg
    12541 taccacatta caggcacaaa accgcgccat cgacgccaaa gagatcctga tgaagcgcaa
    12601 aatcactgcc gcaccggtgg tagatgaaaa cggcaaactc gtcggcgcaa ttaacctgca
    12661 ggatttctat caggccggga ttatttaatc cttcaatccc agacgtttcg ccagccgatg
    12721 caggttggcg acgtcggttt ccagcattcg cgcgcaggct gcccagttgt gatgattttg
    12781 tgccagtgcc tgacgaatag tttcacgctg gaacgcttct gtcgcttcac gcaggttttg
    12841 cttaacaacg ggcaccgccg ccgcttctgg cggcggtaac gtcacctcag gaaaagcaaa
    12901 atgttgcgcc tcaagaatca cttcatcgcc gttgcgggtg gctctcgcca gaactaccgc
    12961 ccgatgaata gcatgttcca gttcgcgcac gtttcccgga aaactgtagt gttgcagtaa
    13021 atttcgcgct ccggcactta ataccacgcg ggagagcccc tgccgcaaac gacactgctc
    13081 gcagaaatac cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga
    13141 aagtggaaac acgctcaggc ggtggaacaa gtcagcgcga aatcgccctg ccagcacctc
    13201 ttcgcgtaaa tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aactacggtc
    13261 atcgccaacg cgctgaatat cgccatactg caacaccctc agcagcttgg cctgcaatgc
    13321 caacgacaac tcgccgatct catcgagaaa cagcgtgccg ttatccgcca tttcaaactt
    13381 cccactgcgg ttactgatag cgccagtaaa cgctcctttc acatgcccga acaactcact
    13441 ttccgccaca ctttccggca gtgcggcaca gttgagatag accagcggat tcaccgcccg
    13501 tggcgaggcc tcatgaatcg ctttcgccac cagctcctta ccggttcccg tctcaccgct
    13561 gatcaggacg ttgagatcgg acgccgccac aatctcaatc tcttttttca gttgcgtcat
    13621 gcctggcgac agaccgatca tctgcgtctg tttcaccgct tcaaatggag cggcatcgtc
    13681 tggcagcata ttctggcttt ccagttgttc aatcagcaac gcattgctta acgctcccgc
    13741 cgccagcgcg gcaatcaggc gtaactcttc gtcgctgaaa acatcgaact gatcgggctg
    13801 catcccgtcg agcgtcaatg cgccgatcag gttttgcccg gcaaacagcg gcagaccaac
    13861 gcaggcgtga accttcagac tctcctgccc tggaatcaaa ccgtcatagg gatcgggcaa
    13921 ttcgctgtct gcgggaaagc gcaccacatc acccgcgcgg gcaatcgctt ccagccgtgg
    13981 atgaccttcc agcgcaaagc gtctaccgag tacatccttc gccagcccgt cgatggcaag
    14041 cggaataaac tgccgcgagt cgtaacgtag caacgcagac gcatcgcact ccagcacctg
    14101 acgcagcgtg gtgatcaggc gctgaaaacg atcctgatga ccaatcccac gctgcaattc
    14161 gatggcgata ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg
    14221 actatagata ttgtcatatc gaccatttga ttgatagtca ttttgactac tcattaatga
    14281 gcataatttt atttatagag taaaaacaat cagataaaaa actggcacgc aatctgcaat
    14341 tagcaagaca tctttttaga acacgctgaa taaattgagg ttgctatgtc tattgtggtg
    14401 aaaaataaca ttcattgggt gggtcaacgt gactgggaag tgcgtgattt tcacggcacg
    14461 gaatataaaa cgctgcgcgg cagcagctac aacagctacc tcatccgcga agaaaaaaac
    14521 gtgctgatcg acaccgtcga ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat
    14581 gaaatcgatc tggcggatat cgattacatc gtgattaacc atgcggaaga ggaccacgcc
    14641 ggggcgctga ccgaactgat ggcacaaatt cccgatacgc cgatctactg tacagccaac
    14701 gctatcgact cgataaatgg tcatcaccat catccggagt ggaattttaa tgtggtgaaa
    14761 actggcgaca cgctggatat cggcaacggc aaacagctca tttttgtcga aacaccaatg
    14821 ctgcactggc cggacagcat gatgacttac ctgacaggcg acgcggtgct gttcagtaac
    14881 gatgctttcg gtcagcacta ctgcgacgag catctgttca acgatgaagt ggatcagacg
    14941 gagcttttcg agcagtgcca gcgttactac gccaatatcc tgacgccgtt cagccgcctg
    15001 gtaacgccga aaattaccga gatcctgggc tttaacttac cagtcgatat gatagcgacc
    15061 tcccacggcg tggtctggcg cgataacccg acgcaaattg tcgagctgta cctgaagtgg
    15121 gccgctgatt atcaggaaga cagaatcacc attttctacg acaccatgtc gaataacacc
    15181 cgcatgatgg ctgacgctat cgcccagggg attgcggaaa ccgacccacg cgtggcggtg
    15241 aaaattttca acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt cttccgctca
    15301 aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga tgatgccgaa aatcgccggg
    15361 ctggtggagg agatgaccgg attacgcttc cgtaacaaac gcgccagtgc tttcggctct
    15421 cacggctgga gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc
    15481 gaaatgtcgc ttagcctgaa agcgaaatgg cgaccagacc aggacgctct ggagttatgc
    15541 cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg
    15601 gtgaatacgg tagttaaaga agaaacctct gccaccacga cggctgacct cggcccacgg
    15661 atgcagtgca gcgtctgcca gtggatttac gatccggcaa aaggcgagcc aatgcaggac
    15721 gttgcgccag gaacgccgtg gagtgaagtc ccggataact tcctgtgccc ggaatgctcc
    15781 ctcggcaaag acgtctttga cgaactggca tcggaggcaa aatgagcaac ggcattgtga
    15841 tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca
    15901 gtattccatt aaccctgatt gccgccgaca gcatggatga gtacaacaaa cctgacctcg
    15961 gccatgttat cagtcagggg caacgtgccg acgaccttac ccgccagacg gcgggtgaat
    16021 ttgccgagca gtttaatctg cgcctgtttc cgcacacctg ggtgacggat atcgatgccg
    16081 aagcccatgt agtgaaaagc cagaataatc agtggcaata cgacaagtta gtgctggcaa
    16141 ccggtgccag cgcctttgta ccgcctgtgc ctgggcgtga gttaatgctg acgttaaata
    16201 gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg
    16261 ttggcggtgg tttgattggt agcgaactgg cgatggattt ttgtcgtgca ggcaaaatgg
    16321 ttacgctaat cgacaacgct gccagtattc tggcgtcgtt aatgccaccg gaagtaagca
    16381 gccgcttgca gcatcggttg acggagatgg gcgttcatct gctgttgaaa tctcagttgc
    16441 aggggctgga aaaaacggat tctggcattc aggcaacgct ggaccgccag cgcagtatcg
    16501 aagtggatgc ggtaattgcc gccaccggac tgcgcccgga aaccgccctg gcacgacgcg
    16561 ccggactgac gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg
    16621 atatttacgc gctgggcgat tgcgcggaaa ttaacggtca ggtactgccg ttcctgcagc
    16681 cgattcaact tagcgcgatg gtgctggcaa aaaatcttct cggcaataac acgccgctga
    16741 aactaccggc gatgctggtg aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg
    16801 aaacccagcg tcaggattta cgttggcaga ttaataccga acgccaggga atggtggcgc
    16861 gcggcgttga cgatgctgac cagcttcgcg cctttgtggt cagtgaggat cggatgaaag
    16921 aggcatttgt attgttgaaa acgttgccga tgtaggtggg ctactgtgcc taaaatgtcg
    16981 gatgcgacgc tggcgcgtct tatccgacct acggggagcg catatgtaag ccggataagg
    17041 catttacgcc gcatccggca agggtgtcca aatgcaacac gttttatccg ttctggactt
    17101 cacccgctaa ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac
    17161 ccgccggtaa actctgtgga aagagcaatg tgaaatcagc gagataatgc gccagacgtg
    17221 cacgcagcaa acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca
    17281 tcgtggcctg ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc
    17341 acgcgcgttg attaaccggt gcctgccagt tcagccactg ctgccagaaa gtggcgagat
    17401 ccagttgatt gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg
    17461 ctagcgcctc cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc
    17521 ccagtgccgc cgccacagca tcgaacaaac gcccacacga tgacgccagc ggcgcgttaa
    17581 ttccacgctc aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt
    17641 cagggtaatt ctgccactcc ggcacaaagc gcaggcactg cgccagcagg ttacgccacg
    17701 gctgcttcgc cgccaaatcg ccaccagcaa gcgccactgc aggcaagccg cccaggtgct
    17761 cacattcgcg atagttcacc agcaggcact cgccgcccca caaagcgccg ttctccccca
    17821 taccgatacc gtcgagcgtc aaagcaatga catcaccgcc ctccagtggc cagtgatgtt
    17881 ctgccagaca cgccgccgcg tgggcatgat gatgcagcac cgtttgcgtc ggcagattca
    17941 tttcgctcgc ccactggctg gagacatagc caggatgtgc gtcatgcaca acgtattgcg
    18001 gggtgaaatc gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc atctggatgc
    18061 catcgtcact taaatcgccc agatgctggc tcaacaccac ttgttcaccg cgcaccaggc
    18121 agaaggtatt tttcagatcc gcgccgagac acagtacagg cggaacattt ttaaagcccg
    18181 gaggcaaagc cagcgcatcc ggcacatacc cccgcgaacg gcgcatcatt tcgccgcttt
    18241 cgcgcaccac cgaatcatcc atccgctgta cgatgtcgcg gttatgtatc aagaatccgt
    18301 cggcaatgcc ctgcaaatcc tccagcgcct gttcattgct gatagccggt ggtttaccgc
    18361 tcaggttgcc agaggtcatc accagcgggc attgcagttc ctgtaacagc aaatgctgaa
    18421 gcggattcgc aggcaacatt accccgactt cgttaaggcc aggggcgata tcatcacaaa
    18481 tctcaggtac gtatttttta tccaccagca caatcggcgc ggcgggagtg gtaagcaact
    18541 ggcgcgcagc gtctggtaaa ccgtcagcca ctggcaacat gaccgccaac ggtttcgccg
    18601 ggcgatgttt gcgcgcccga agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa
    18661 gatgaaatcc gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag
    18721 ccgcctgtaa tgccgcctct tgttccgcat gttcaccatg acttacccat tcaagatgtg
    18781 ggccacactc cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt
    18841 actctttatc acaggctgga cacagcggaa acgccgccat tacggtaaac gggcggtcgt
    18901 aaggcatggc gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg ataaacggat
    18961 aacgataacg ccgttcgcct ggcgtattca tttcggcaag acaggcaggg caagtggcgg
    19021 catcgggaac aatttgcgta ttcatggtgc cgcctgtgct ctggcgtata gtgaactcgg
    19081 tgggcagttg tgaccagata aacggctcac gctcgacgct atcaatacgc gccagcggcg
    19141 ggcagtgctg atgcaattga acaagaaacg tttccgggtc ttccagcagc cggacttcca
    19201 cgccatcgcc gtcattacag acatcgccgt gaagatttaa ttgctgtgcc agttgccaga
    19261 caaacggacg aaaaccgacg ccctgcactt tgccacgaat acgcagttgg acaccgcaag
    19321 atgtgttttt tgccattgag ttattcccgc catcatgaat tgcgtaaccc gcccagccgg
    19381 acacgacagc gtcgtatccg gcagtctcag gtcggcgata ccgcccgctc cgtattctac
    19441 gaatatttcc gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag
    19501 cgccgtgcgg cgacgttttt ctgcgctcag ttgttcaagt ttattacgat cgacacaaat
    19561 cagcgcatga gtcgggcaag ccgccataca cgccggaccg tcttcacgat ggttgcacag
    19621 gtcgcattta ttggcttcgg ctttgtcagc ccgtacattc agccctgcgc cgctgttgcg
    19681 aatcaccgga cgtaccacca cttccatcgc gccatacggg caagccacaa cgcaggtttt
    19741 gcaaccaatg caacgttcct gcatgacatg aacaaaccct ttatcacggc tgatagcacc
    19801 attcgggcag acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga
    19861 aatgttcaca cctttaatga catggattcg cggtaaaaaa gtttccgggg tcagcgatgc
    19921 acagtcctga ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat
    19981 acatttactc gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtta
    20041 atgcgccgat acattcacaa atcatgccag tttttaattc actgttattt aaggaaatta
    20101 atttctgtaa tgcaggaaaa aaagatgtca tcgacactag tgacgatgac atgtgatgac
    20161 aatgtttatc gcgaaagagc aatgagtgag tcgcggcgga tcagttttcc gctgaaggtt
    20221 ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat aatttcctga
    20281 atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata gggggcaatt
    20341 gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc gcgcttgtgt
    20401 aacgctttca tcgcacctat cgccatatcg tcgttactgg caactaacgc gctaaattta
    20461 gccccacgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt ccatttaccg
    20521 ttagcgataa gtttttcatt gagcgcaata ccatgctgcg ccagcgcgtc tttatacccg
    20581 gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc aatctcctga
    20641 tgtccggcgt ttatcaactc tgccacggca ttaaagctgg tctgtttatg atcgcaccag
    20701 acgctatggc tgctattttt gcgcaggcgg cgattaagca ccattatcgg ctgactgtgc
    20761 gcgtcaataa tgtcatcgat ctcatccacg cttaaaaaac gcgggtaaat cataatggcg
    20821 tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc gctgtgttta
    20881 ccatctgcca atagcaactg ccgccctttc tcttccgcca ttcgcgcggc atgaaagagt
    20941 aattcactaa aataaatgcc gtggtaaagc gtgttagtca ctaccagccc cagcgtctga
    21001 gtactcttcg ccgacagatt gcgcgctagc aagtttgggc ggtaaccgct ctcttctacc
    21061 gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga aagcacgcgg
    21121 gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt cgtcatcatt
    21181 ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt gtccttgcgc
    21241 aatcggcggg aaaaatattc aggtgaccgg tttcacaaat ataaaaaatg aacaattaac
    21301 tctcttgctt atttggtgac aactattcat gattttgtga aaccggtttc ttaattccgt
    21361 ttcagcatcg gcatttttcc gtcacgtcga ctgataacaa ctacatctac cctactgata
    21421 acaggataaa atccgatggc caaaaattat gcggcgctgg cacgctcggt gatagcggca
    21481 ctgggcggcg ttgataacat ctcggcagtc acccactgta tgacgcgttt gcgctttgtt
    21541 atcaaagatg accagcttat cgacagcccg acgttaaaaa ccatccccgg cgtgctcggc
    21601 gtggtacgta gtgacaacca gtgtcaggtg attatcggca ataccgtttc acaagccttt
    21661 caggaagtcg tcagcctgct gccgggagat atgcagcccg cacagcccgt gggtaaaccc
    21721 aaactcacgc tgcgtcgcat tggtgcgggg atcctcgatg cgctgatcgg caccatgtca
    21781 ccgctgatcc cggcgattat cggcggatcg atggtcaaac tgctggcgat gatcctcgag
    21841 atgagcggcg tgttgacaaa aggatcgccg accttaacca ttctgaatgt gattggtgac
    21901 ggtgctttct tcttcctgcc gctaatggtc gcggcatctg ccgccatcaa atttaaaacc
    21961 aatatgtcgc tggcgattgc catcgcgggt gtgctggtac atccgagttt tattgaatta
    22021 atggcgaaag cggcccaggg tgaacatgtc gaatttgccc tgatcccggt caccgcggtg
    22081 aaatacacct acacggtgat cccggcgctg gtgatgacct ggtgcctgtc atatatcgaa
    22141 cgctgggtgg atcgcattac accggcggtg accaaaaact tcctcaagcc aatgctgatt
    22201 gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc cgattggtat ctggatcggt
    22261 agcgccattt cggcgctggt ttacaccatt catggttatc tgggctggct ttcagtcgcc
    22321 attatgggcg cgctgtggcc tctgctggta atgaccggga tgcaccgtgt ctttacgcca
    22381 accatcattc agaccattgc cgaaaccggc aaagaaggga tggtcatgcc gtcagagatc
    22441 ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg cgtggaaaac gaaaaacccg
    22501 gaactgcgcc agacggcgct ggctgcggcg gcatcagcca ttatggcggg gatttccgaa
    22561 ccggcgttat acggcgtggc gatccgcctg aaacgtccgc ttatcgccag tcttatcagc
    22621 ggttttattt gcggcgcggt tgccggtatg gcggggcttg ccagccactc aatggcagcg
    22681 ccggggttat ttaccagcgt gcaattcttc gatccggcga atccaatgag catcgtctgg
    22741 gtgttcgcgg tcatggcgct ggcggtggtg ctgtcgttta tcctcacact gttgctcggc
    22801 tttgaggata ttcctgttga ggaagcggct gccgaggcgc gaaagcatca gagcgtacaa
    22861 ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa aatgtcagta tttccagaag
    22921 gttttttatg gggcggcgcg cttgccgcca accagtctga aggtgcgttc cgtgaaggtg
    22981 gcaaagggct gaccactgtc gatatgatcc cgcacggcga gcatcgaatg gcggtgaaac
    23041 tggggctgga aaaacgtttt cagttgcgcg atgacgagtt ttatcccagc catgaggcga
    23101 cggattttta tcatcgttat aaagaagata tcgccctgat ggcagagatg ggattcaaag
    23161 tgttccgtac ctcaattgcc tggagccgcc tcttcccgca gggtgatgaa atcacgccca
    23221 atcagcaggg cattgctttt tatcgttctg tctttgaaga gtgtaaaaag tacggtatcg
    23281 aaccgctggt cacgttgtgc cacttcgatg tgccgatgca tctggtcacc gaatatggct
    23341 cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta cgcccggacc tgctttaaag
    23401 catttgatgg tctggtgaaa tactggctaa ccttcaatga aatcaacatt atgttgcata
    23461 gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga aaatcaggat caggtgaaat
    23521 atcaggccgc gcatcaccag ctggttgcca gtgcgctagc caccaaaatc gcccatgagg
    23581 ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg caacttctac ccttacagtt
    23641 gcaagccgga agatgtctgg gcggcgctgg agaaagatcg ggaaaacctg ttttttatcg
    23701 atgtgcaggc gcggggcgcg tatccggctt actctgcccg cgtattccgc gaaaaagggg
    23761 taaccatcga caaagcaccg ggcgatgatg aaatcctgaa aaacaccgtc gattttgtct
    23821 ccttcagcta ttacgcctcg cgatgcgctt cggcggagat gaacgccaac aacagcagtg
    23881 cggcgaacgt ggtgaaatcg ctgcgtaacc catatttaca ggtaagcgac tggggctggg
    23941 gaattgatcc actcggtctg cgtatcacca tgaacatgat gtacgaccgc tatcagaagc
    24001 cactgtttct ggtggaaaac ggcctcggcg caaaagatga atttgctgcc agtggcgaga
    24061 ttaacgatga ctatcgcatc agctatttac gcgaacatat ccgcgcaatg ggcgaagcga
    24121 ttgcagacgg cattccgctg atgggctaca ccacatgggg ctgtattgat ttagtttccg
    24181 cctctacggg tgaaatgagc aaacgctacg gttttgtctt tgttgaccgt gacgacgcag
    24241 gaaacggtac gctgacgcgc acgcgtaaga aatcattctg gtggtataaa aaagtgattg
    24301 ccagtaatgg ggaagattta gagtaagtaa ccgtgccgga tgcggcgtga acgccttatc
    24361 cggcctacat agttcgtgcg acatgtaggc ccgataagcg cagcgcatcg ggcaatctgg
    24421 ggattggcat cagtcaaaaa tcaaagaaac cattcaggct taacgccaaa tcgggctgaa
    24481 agtgccttaa tatgggatat tgtcagggat cgttgtcctg acaaaatctg actgaccagg
    24541 gatttagagc cgatttcgtt cttcaaatcc gcataggtca gattatgttg atcaatcagc
    24601 gtacgaagta acgctacgcc aaccggcatt gccgcaacag ctttgtcgaa ctctgcaaac
    24661 ttttcgttgt tgttttcata ctctgcaatg cggctcgcca gaaagtcgat aagtggattg
    24721 gtgtcatctt cttcaatcaa atattcgacc agttccagtg cttcacgata atcatcttct
    24781 gagtcactgc ctcccagaaa ggggactgca ttgaccagtt ctcttgttgc tttaacagcg
    24841 cgtgcagcat tagcagtcat tctttgttct ctctgtagta tcgtgtcaga cgcccacttt
    24901 tctaaaatgc caggtttgct cggcatcata aggcatggtc aggagaggtt tcagcgcccg
    24961 cttttgcgcg tattcgctgt cactcattgg cagatattcg gtaaagctgg tggcctgtgc
    25021 aggcagcact aagctcaata aaaaagcgca catccctgtt ctgatgttca attccctgaa
    25081 ttccctaatg aagaattatt cttccaccgc taactgcgcg aagccgccgt tcccttccca
    25141 gccttccagt cgttgataaa cggtttctac cgcatcttta atcggctggg tcatcgggta
    25201 gtaaaagccg acgatatccg gctggatgcc gaggaaaatc acttcgccaa tgtcttcttt
    25261 caactgatcg ataaggtagt tcaacggcat gttatgggta gtcatcataa acatctcagc
    25321 gatatcatcc gggtcgatga tgcggatttc gccggggttt agccccatat ccgtggcgtc
    25381 gacaatcagt aaccgcgtag ggcgcagttc acggatggcg acgatgtcgt tttccggtgc
    25441 actaccgcca tcaatcacca cccagttacc tttcggcgcg gcagcgcact tttccgccag
    25501 cagcggacct gcgccatcat cgcccatcat gctattgcca acacagagta aaacgtcagt
    25561 cacgcagtct cctcaccatc aaatagatgg cgttttcctg atgaatatca tgcaacatac
    25621 ttaacagcat tgtactccat tctttctgct gcgaagtttg cgctgcccgg gctttatcga
    25681 atgcgttggc cagcatcggc acatggttaa tgtcgatgac gatttcacca tacttcggca
    25741 caccttccat tttgcggcgg gcttcgctgc ccgcctccag agtggcaatc catgccagat
    25801 attcatccca cgggcaggtg agcgctgctt ccaggcaatc gataacccca aggtggtgac
    25861 caatcgccag gctgtaatag accacctgct gcgcctcggc gggcgtggca tcgttctcat
    25921 caataaattt acggctcagt tgactgaaca ccaccttttc actcatcgga tacgcgcctc
    25981 ttcaacaaca tgattcagat ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc
    26041 cagccagcgt gcaacctgtt cttcgccctg ccctaactgt gtaaggtaat catcggcaat
    26101 ctgacgaccg taacgataac ccgccagtcg acgtgcttcg cgatccactt tcacacgcag
    26161 cggctgcacc atatcgccat gcaagatctc cgccggttgt tcatcctgct cacccggccc
    26221 acgggcgtga attttctgct ccagcaggcc gagcgccatt gcaaagccgt acagcgtggc
    26281 ggctggcgtt ggcgggcagc cagggatata aacatccacc gggacgattt tatccgtacc
    26341 gccccacacg cagtagagat cgtggaagat cccgccgctg ttaccgcagg caccgtagga
    26401 gatacaaatt ttcgggtccg gcgcggactg ccacgcacgc agcgcagggg atcgcattgc
    26461 acgggtgacc gcgccggtaa acagtaaaat atccgcatga cgcggtgaag gaacgacttt
    26521 aatgccgaag cgttctgcat caaacagcgg cgaaagcgtg gcgaaaattt cgatttcgca
    26581 gccgttgcag ccgccgcagt ccacgcggta aacgtaggca gaacgtttga tttttttcag
    26641 taacgacgcc ttcatgctgg cgatggattc atccaccgtc atcgggaccg gaatgccgtt
    26701 ggcgtcacgg gggcctaata aattgctcat cagatggcct ctttcatatg gcgagtcagt
    26761 tcaatacggt cggacggcac caggcatttc tggcgcttac attccgggca agtctcaaag
    26821 ctttcgcggt ggttttccgc gcggctgtca ccattgtgct taagcagagc aatagcgtag
    26881 tcgatctctt tctggacggc gaaaggacga ttgcagacgc gacagttgca cagcgcgaag
    26941 cgggactgtt gcaggaagtc ttctttcttc cacaccgcca gttcgtactc ttgcgacagt
    27001 ttgattgccg ccgtcgggca aacttcttcg cagcgtccac agaagatgca acgcccgaga
    27061 ttaaactccc acgcaagctc tcctgtggcg aggtcagttt caaccgttaa ggcgtttgac
    27121 gggcaggcat tgacgcaggc cgcgcagccg atgcactgct gcgggttctg ctctggctta
    27181 ccacggaagt ttttatcaac cgcaatcggc tccagcggat aagacgaggt cgccgtgccg
    27241 gttttgatga cttttttgat aaaggtaaac atggcgattc cttatttcag cggcgagttt
    27301 ttacgctcaa tgctgtaacg ctcgagttct ttgtacggca ccactttgct cttcttctta
    27361 cgcacatcga ccacggtcat gcggtcggta caggagtagc aagggtcgag gctaccgata
    27421 atcagcggcg catcggaaac ggtattgcca cgcagcatgt agcgcagggt cggccagttc
    27481 gcgtaagtcg cggcacggca gcgccagcgg tacagcttct ggttgtcgcc agtcatgctc
    27541 cagtggatat catcgccgcg cggcgcttcg gcaaagccca gcgcaaagcg gtgcggaatg
    27601 taggtaaagc cttccaccat cagtgggcca cccggcaggt tatccagacc gtagtcgatc
    27661 atgttcagcg cggtatagac ttcgttgata cgcactttca gacgggaaat aacgtcgcag
    27721 ccctgctcgc tgtggacttc cattggcagc aggccataac caacaaacgg gtgatcggcg
    27781 cgagtatcgc gagcgtgacc gctggcacgg accatcgggc cgacgttact gaagtcgcga
    27841 gcgatttccg ggtccagacg accaatgccg acagtgcgtt gttccatgtt cggtgtgctc
    27901 aacagcacat ccaccagctc ctgcacttca cgacgcatct gttgcgccag ctggcgggtc
    27961 tggatcatgt catctttcag cagatcgcga cgaatcccgc cgatcaggtt caggccgtag
    28021 gttttacgcg caccggtaag gatctctgcc attttcatgg aggtttcacg cacgcggaag
    28081 aactgcataa agccggagtc aaagccggta aagtggcagg caagaccgag gttgagcaga
    28141 tgcgagtgca ggcgttccac ctccagcaga atggcgcgga tcatctgcgc acgttctggc
    28201 acctgaatac ccatcgcgtt ttccaccgac gtggtgtagg cggtgctgtg agcaaagccg
    28261 cagatcccgc acacacggtc agagaggaag gtcacttcgt tgtaacccat acgggtttcc
    28321 gccagttttt ccatgccgcg atgaacgtag aacagacggt agtcggcgtc gataatgttt
    28381 tcgccatcga cgaacagacg gaagtgaccc ggttcgtcag aagtgacgtg cagcggacca
    28441 atcggcacga cgttgttttt cttgtcgccc agttcgttga tgaactcgta ggtttcagca
    28501 tcggtggtcg gtgccggacg ttgacgataa tccatgctgt ctttacgcag cggataaagt
    28561 tcatccggcc agtcatccgg cagcaccaga cggcgttcat ccggcagccc taccggaatc
    28621 aaaccgtaca tatcgcgcac ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc
    28681 acggacggat actccggttt gttggcatca acttcgacgc gaaccgttac ccaacacttg
    28741 gtgcccttct ccatcgacag cacgtaataa acggcgtaat gaccattcag tttgcgttcg
    28801 tcgttaccaa acagcaccga cagccagcca ccctgtttgt agtaaagaaa ctccaccact
    28861 tccggcaggt agttcacctt tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg
    28921 tccagcacga cgcccggaaa tgcctcattc agcgcggcga gataatgttg acctaatttt
    28981 tcttcagaca tgctcaaact ctctttaatc acgccgccag caaggagacg aacgctaaaa
    29041 atgcaaagcc aaacccagcc caggtaatgc gcggagtaat atcaagacgc agacgggcca
    29101 tgctgttttc gaacagcgcg ataaccagga cgccgaccac cagtttgacg atggcaatca
    29161 ccaacgccag cagcagccca ccgacggtga aggtttccat ttgtccccac ggaataaaca
    29221 ccccgacgaa catctgcagc accaccaact gtttcaggct gataccccat ttcatgacgc
    29281 caaagccgct gccgctgtac tcagagagcg gaccttcctg caactcctgc tcggcttccg
    29341 ccaggtcgaa cggcagtttg cccatttcga taaaagtggc gaacgcacag gcacaaagcg
    29401 ccagcaccag cgggatgctc tggctcagcg gccagtgata aacggtgtcg gtgatgttgc
    29461 tgatgttggt ggaaccggca acctgtgcgg caacccacag accaagcagc agcatcggtt
    29521 cgaccagcac gccaagcatc gcttcacggc tcgcgccgat agcagtaaac gggctaccgg
    29581 tatccagacc ggaaatggca aagaagaaac gcgcgatggc aaagagatac agtaaggtga
    29641 tcaaatcgcc cagttgcggc agcggagaac caacggtcac caccggcagc gcagtagcga
    29701 tagtcagcat gacgcccacc atcacatacg gcgtcaggcg gaacacccag ccggaggcat
    29761 ccgggccaac gctctgacgc cccagcagtt tgataatgtc gcgatactcc tgcaacacgc
    29821 ccggcccgcg acggttatgc aagcgggcgc gcgccacgcg ggttataccg gagagcagcg
    29881 gcgcaacggc aaataacacc agcgcctgaa ttaacggata taaaacactc attctcaggc
    29941 tcctcgtgaa acaataatca ccaccagtac cgccagttca accagcgcca tccggcggaa
    30001 cagcaacgca ctcccctcgc actgccagcc cggcaccagc gacaccggat tcagccattt
    30061 gcgcagtttc agcaccggcg caaacgcctg tttcaccggc atggcaaaac cgtgagcggt
    30121 aatcaccatt gatttttcgt ggtcgtagcc gcacacccag gccgcgccgc gggaacgcga
    30181 tggcaaacga tcgcctttgc aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat
    30241 cagcagcaac gtgatcatcg gttgagaaac ggtggtgtta gcaggctcca gcggcagagg
    30301 tacagcagca gagagcatcg gcaatagcca cggcgcagca acaccaccaa ttacgcagca
    30361 aatcgccagt gccactacgc ttacgctcat caggagcggc gcacaggtgg cgttttcggc
    30421 ttctttggta cgcggcgcac cgaggaacgt gacgccatag actttcgcca tacacatcac
    30481 cgccagcgca ccggtaattg ccagatccac agcaagcagt ggccccagca gacgggcaac
    30541 aaacgcgcca ctattgctca gtttgaaaaa ggattgatag ataacccatt ccccggcaaa
    30601 cccgttcagc ggcggcagcg cagccattgc catcaacccg actaacatgg cgatggagat
    30661 aaccggcatt ttcttgccaa taccaccgag tttttcgata tcgcgatgac cggtacggaa
    30721 ccagacgctc cccgccccga ggaacagtac gcttttgaac aggctatggt taagcagatg
    30781 gtacagacca ccgaccaggc caagagcaat cagcgccggt tgttcgagcg cgatacccgt
    30841 tacgccagcg cccagcccca gcaggataat gccgatattt tccagggtgt ggtaagccag
    30901 tagacgctgg atattatgtt ccatcaacgc atacagaccg ccgacaaacg cggtgatcat
    30961 gccgagcacc agcagcgcga tcccccacca cagcggtgca ttaccgccca gcagtgacag
    31021 ggttaaaatg cccagcaggc caattttcat gactaccgta gaaaacaacg cggcagctgg
    31081 cgcagaggcg ttcgcatgtg cctgcggcac ccagccgtgc agcggaataa tcccggccag
    31141 caggccaaag ccaatcacac cgagcagcca gatatcggaa ccgagcggca gctgttgcat
    31201 acgcatatcc agcaggcgca gatccagcgt gccgtaacgc tgccacagca gccagcaagc
    31261 aatcgccagc agcagagtgc caagacgccc cagcgcaaac cacagtttgc cctctttgct
    31321 gttgctggtg aggaacaccg cacacaaggc catgacttcg gccattacca cgaacatgcc
    31381 gaggttgctg gcaatgacgg cgcagacggc ggcagccatc aacatattga tctgcaagcc
    31441 gttgcacttc acctgcgcgt ggcgatgcca gtcaatgttg tagaggctga caaacagacc
    31501 gcacagaccg agcgtaatca gccaaatcgc gttaagcgga gagatttgca catcgtagct
    31561 taccagcgac agcgcaccgc tcacgccaac cgcgccagtc agtacagtga agcccgcggc
    31621 tgccgtatac agactaccaa ccgcgccgcc aattccggct atccagccgc ttagcgcctt
    31681 ttgaaaagaa aagagaaatg ccagaatagc ggcggcgaca aaccacgcca cgccgctatt
    31741 gatcagggaa attgcgctca tttagcctct ccactttgag cctgctgaaa caaggtgaga
    31801 tcgccaaagt ccgtgttaaa ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg
    31861 gtgttatcca ccagatgcag ggctttagtc gggcacatcc gcacgcaggc cggaccttgt
    31921 tcatcaaagc tacaaaggtc acatttgacg gcgatcgcgc gaatacctgg cacccagtca
    31981 agcaatgtgc tgacacgcgc cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg
    32041 tttgccggaa tatccagcgg acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg
    32101 atgccgcaca gtttgcagct tacgcacagg ctttcattca actgcacggc cccatcaacg
    32161 cgggtgatgg cgttaaccgg gcagaccacc gcgcagggtg catcttcaca gtggtgacag
    32221 agctgcggcg cagattcttt ttcatttagc atcactctca ggcgcggcat tgattgcagg
    32281 ccgtgctggc gatgcgtctc tgaacaggcg gcctcacaag tgtggcagcc gatacagagc
    32341 gtggagtcag caattacaaa acgattcacc aggcattcct caggtgattg tcatttttga
    32401 cgaaaacatg ccgatgaaat gtcattttcg acactcatcg acacgcccat ccccaaacag
    32461 gcgtaacgcc tgcaaaacgg gcaaagcctc agctcatgct gccgggcttt gtccctttac
    32521 cagttggctt aaattcaccg gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac
    32581 gccagcgatt ttttcgagat agtgttccag aacctgttcg cgatcgcgga tgttgacctt
    32641 gccatcaatc atgccgtcaa tgctcagttg tgcctgcgca atccgctgtt tgtcttcgcc
    32701 atcttttgtt tcgacgtaat actcgatggt gtggctgtta aagccatccg ccagggtgac
    32761 gtaaatgcga aaatgttcaa aaaggacgaa acagagttct ttgtccggcg cgcagctcat
    32821 ggcgtgatgc aggcgcgctt tcgataacgt gatcccctga accagcgaat tgcagtagat
    32881 gtgccactgg tcctgtaggc gacgatgccg ctgtgcgatg taatcggctt tctcgcttat
    32941 ttcccaaata gtcattgtca ggttacccgt ttaacagaga tgccagcttt aagcattttt
    33001 tgtgccaact tttaatttat tgttattaaa gcgattttta agattttggt gaatttcgtc
    33061 gccgtgtcga cgtgtcattt cgacatcatc gacattattc actgcaggga taatcaacac
    33121 tggcacaatt attgcttgta gctggcaata gttaatggga ggcgatatgc acgaaataac
    33181 cctctgccaa cgggcactgg aattgatcga acagcaggcc gcaaaacacg gcgcaaaacg
    33241 cgtaactggg gtctggctca aaattggcgc attttcttgt gtcgaaacca gctctcttgc
    33301 cttttgtttt gatctggttt gccgcggcag cgtggcggaa ggttgtaaac tgcacctcga
    33361 agaacaagag gccgaatgct ggtgtgaaac atgccaacag tatgtgacgc tactgaccca
    33421 gcgcgtccgc cgctgtccac agtgtcatgg tgacatgcta cagattgtgg cagacgacgg
    33481 tttacagatt cggcggatag aaatagacca ggagtgagcg atgtgtacaa catgcggttg
    33541 cggtgaaggc aacctgtata tcgagggtga tgaacataac cctcattccg cgtttcgtag
    33601 cgcaccattt gccccggcgg cacgcccgaa gatgaaaatc accggcatta aagcgcctga
    33661 atttaccccc agccagaatg aagaaggcga cctgcattac ggtcatggcg aagcgggcac
    33721 tcacgcaccg ggcatgagcc agcgtcggat gctggaagtc gaaattgacg tgctggacaa
    33781 aaataaccgt ctggctgaac gcaaccgcgc gcgctttgct gcccgcaagc aactggtgct
    33841 caacctggtt tccagcccag gttccggtaa aaccaccctg ctgacggaaa ccttaatgcg
    33901 cctgaaagac agcgttccgt gcgcggttat tgaaggcgat cagcaaaccg tgaacgatgc
    33961 cgcacgcatt cgcgctaccg gcacaccagc gattcaggtg aacaccggta aaggctgcca
    34021 tcttgacgca cagatgattg ccgacgccgc accgcgcctg ccactggacg ataacggtat
    34081 tctgtttatc gaaaacgttg gcaacctggt atgcccggcc agcttcgatc tcggtgaaaa
    34141 acacaaagtg gcggtgcttt ccgttaccga aggtgaagac aaaccgctga aatatccgca
    34201 tatgtttgcc gccgcctcgc taatgctgct caacaaagtt gacctgctgc cgtatcttaa
    34261 ctttgacgtt gaaaagtgca tcgcctgcgc ccgcgaagtc aatccagaaa ttgaaatcat
    34321 ccttatttcc gccaccagcg gcgaagggat ggaccagtgg ctgaactggc tggagacaca
    34381 gcgatgtgca taggcgttcc cggccagatc cgcaccattg acggcaacca ggcgaaagtc
    34441 gacgtctgcg gcattcagcg cgatgtcgat ttaacgttag tcggcagctg cgatgaaaac
    34501 ggtcagccgc gcgtgggcca gtgggtactg gtacacgttg gctttgccat gagcgtaatt
    34561 aatgaagccg aagcacgcga cactctcgac gccttacaaa acatgtttga cgttgagccg
    34621 gatgtcggcg cgctgttgta tggcgaggaa aaataatgcg ttttgttgat gaatatcgcg
    34681 cgccggaaca ggtgatgcag ttaattgagc atctgcgcga acgtgcttca catctctctt
    34741 acaccgccga acgccctctg cggattatgg aagtgtgcgg tggtcatacc cacgccattt
    34801 ttaaattcgg cctcgaccag ttactgcctg aaaacgttga gtttatccac ggtccgggtt
    34861 gcccggtgtg cgtactgccg atgggcagaa tcgacacctg cgtggagatt gccagccatc
    34921 cggaagtcat cttctgtacc tttggcgacg ccatgcgcgt gccggggaaa cagggatcgc
    34981 tgttgcaggc aaaagcacgc ggtgccgatg tgcgcatcgt ttactcgccg atggatgcgt
    35041 tgaaactggc gcaggagaat ccaacccgca aagtggtgtt cttcggctta ggttttgaaa
    35101 ccaccatgcc gaccaccgct atcactctgc aacaggcgaa agcgcgtgat gtgcagaatt
    35161 tttacttctt ctgccagcac attacgctta tcccgacgct tcgcagtttg ctggaacagc
    35221 cggataacgg tatcgacgcg ttcctcgcgc cgggccacgt tagtatggtt atcggcactg
    35281 atgcctataa ttttatcgcc agcgattttc atcgtccgct ggtggtggct ggtttcgaac
    35341 cccttgatct actgcaaggc gtggtcatgc tggtggagca gaaaatagcg gcccacagca
    35401 aggtagagaa tcagtatcgt cgggtggtac cggatgccgg taacctgctg gcgcaacagg
    35461 cgattgccga tgtgttctgt gtcaacggcg acagcgaatg gcgcggctta ggcgtgattg
    35521 aatcttctgg cgtgcacctg acgccggatt atcaacgatt cgatgccgaa gcacatttcc
    35581 gcccggcacc gcagcaggtc tgcgatgacc cgcgcgcgcg ttgtggcgaa gtcttgacgg
    35641 gcaaatgtaa gccgcatcaa tgcccgctgt ttggtaacac ctgtaatcct caaaccgcgt
    35701 ttggtgcgct gatggtttcc tccgaaggag cgtgcgccgc gtggtatcag tatcgtcagc
    35761 aggagagtga agcgtgaata atatccaact cgcccacggc agcggcggcc aggcgatgca
    35821 gcaattaatc aacagcctgt ttatggaagc ctttgccaac ccgtggctgg cagagcagga
    35881 agatcaggca cgtcttgatc tggcgcaact gatagcggaa ggcgaccgtc tggcgttctc
    35941 caccgacagt tacgttattg acccgctgtt cttccctggc ggtaatatcg gcaagctggc
    36001 gatttgcggc acagccaatg acgttgcggt cagtggtgcc attccgcgct atctctcctg
    36061 tggctttatc ctcgaagaag gattgccgat ggagacactg aaagccgtag tgaccagcat
    36121 ggcagaaacc gcccgcacgg caggcattgc catcgttact ggcgatacta aagtggtgca
    36181 gcgcggcgcg gcagataaac tgtttatcaa caccgcgggc atgggcgcaa ttccggcgaa
    36241 tattcactgg ggcgcacaga cgctaaccgc aggcgatgta ttgctggtga gcggtacact
    36301 cggcgaccac ggggcgacta tccttaacct gcgtgagcag ctggggctgg atggcgaact
    36361 ggtcagcgac tgcgcggtgc tgacgccgct tattcagacg ctgcgtgaca ttcccggcgt
    36421 gaaagcgctg cgtgatgcca cccgtggtgg tgtaaacgcg gtggttcatg agttcgcggc
    36481 agcctgcggt tgtggtattg aaatttctga atcagcgctg ccggttaaac ctgccgtgcg
    36541 cggcgtttgc gaattgctgg gactggacgc cctgaacttt gccaacgaag gcaaactggt
    36601 gatcgccgtt gaacgcaacg cggcagagca agtgctggca gcgttacatt cccatccact
    36661 ggggaaagac gcggcgctga ttggtgaagt ggtggaacgt aaaggtgttc gtcttgccgg
    36721 tctgtatggc gtgaaacgaa ccctcgattt accacacgcc gaaccgcttc cgcgtatatg
    36781 ctaataaaat tctaaatctc ctatagttag tcaatgacct tttgcaccgc tttgcggtgc
    36841 tttcctggaa gaacaaaatg tcatatacac cgatgagtga tctcggacag caagggttgt
    36901 tcgacatcac tcggacacta ttgcagcagc ccgatctggc ctcgctgtgt gaggctcttt
    36961 cgcaactggt aaagcgttct gcgctcgccg acaacgcggc tattgtgttg tggcaagcgc
    37021 agactcaacg tgcgtcttat tacgcatcgc gtgaaaaaga cacccccatt aaatatgaag
    37081 acgaaactgt tctggcacac ggtccggtac gcagcatttt gtcgcgccct gatacgctgc
    37141 attgcagtta cgaagaattt tgtgaaacct ggccgcagct ggccgcaggt gggctatacc
    37201 ccaaatttgg tcactattgc ctgatgccac tggcggcgga agggcatatt tttggtggct
    37261 gtgaatttat tcgttatgac gatcgcccct ggagcgaaaa agagttcaat cgtctgcaaa
    37321 catttacgca gatcgtttct gtcgtcaccg aacaaatcca gagccgcgtc gttaacaatg
    37381 tcgactatga gttgctatgc cgggaacgcg ataacttccg catcctggtt gccattacca
    37441 acgcggtgct ttcccgcctg gatatggatg aactggtcag cgaagtcgcc aaagaaatcc
    37501 attactattt cgacattgac gatatcagca tcgtcttacg cagccaccgt aaaaacaaac
    37561 tcaacatcta ctccactcac tatcttgata aacagcatcc cgcccacgaa cagagcgaag
    37621 tcgatgaagc cggaaccctc accgaacgcg tgttcaaaag taaagagatg ctgctgatta
    37681 atctccacga gcgggatgat ttagccccct atgaacgcat gttgttcgat acctggggca
    37741 accagattca aaccttgtgc ctgttaccgc tgatgtctgg cgacaccatg ctgggcgtgc
    37801 tgaaactggc gcaatgtgaa gagaaagtgt ttaccactac caatctgaat ttactgcgcc
    37861 agattgccga acgtgtggca atcgctgtcg ataacgccct cgcctatcag gaaatccatc
    37921 gtctgaaaga acggctggtt gatgaaaacc tcgccctgac cgagcagctc aacaatgttg
    37981 atagtgaatt tggcgagatt attggccgca gcgaagccat gtacagcgtg cttaaacaag
    38041 ttgaaatggt ggcgcaaagt gacagtaccg tgctgatcct cggtgaaact ggcacgggta
    38101 aagagctgat tgcccgtgct atccataatc tcagtgggcg taataatcgc cgcatggtca
    38161 aaatgaactg cgcggcgatg cctgccggat tgctggagag cgatctgttt ggtcatgaac
    38221 gtggggcttt tactggtgcc agcgcccagc gtatcggtcg ttttgaactg gcggataaaa
    38281 gctccctgtt cctcgacgaa gtgggcgata tgccactgga gttacagccg aagttgctgc
    38341 gtgtattgca ggaacaggag tttgaacgcc tcggcagcaa caaaatcatt cagacggacg
    38401 tgcgtctaat cgccgcgact aaccgcgatc tgaaaaaaat ggtcgccgac cgagagttcc
    38461 gtagcgatct ctattaccgc ctgaacgtat ttccgatcca cctgccgcca ctacgcgagc
    38521 gtccggaaga tattccgctg ctggcgaaag cctttacctt caaaattgcc cgtcgtctgg
    38581 ggcgcaatat cgacagcatt cctgccgaga cgctgcgcac cttgagcaat atggagtggc
    38641 cgggtaacgt acgcgaactg gaaaacgtca ttgagcgcgc ggtattgcta acacgcggca
    38701 acgtgctgca gctgtcattg ccagatattg ctttaccgga gcctgaaacg ccgcctgccg
    38761 caacggttgt cgctcaggag ggcgaagatg aatatcagtt gattgttcgc gtgctgaaag
    38821 aaactaacgg tgtggttgcc gggcctaaag gcgctgcgca acgtctgggg ctgaaacgca
    38881 cgaccctgct gtcacggatg aagcgactgg gaattgataa atcggcattg atttaactgc
    38941 aaattgccgg acagatctga ctgtccggca tactattcac gaggtttttt cggacgatat
    39001 ttttccggca gttctggcac cggacgcttg tcatcgatga gatgacgcgc ggttaagatc
    39061 ggatgacgcc acagcattct cggcccggcc caacgcataa tctgtttcat ctcttcacgc
    39121 tttgcaggct ggtaacagtg caccggacac tgcttacagg ctggtttctc ttcgccgaac
    39181 acacatttat ccagccgctt ttgcgcgtaa gcaaacaacg cctcgtaatg ctccggctcc
    39241 gctgacgcct gcgggcattt cgcttgataa agatcgatca tttttttaat cgtcagtttt
    39301 tcacgagaga tacgcttgcc ggacatgctg cctccacctc attaagatgc atttatatta
    39361 caacttaatc ttaaagggca ctatgactct aaagaagaag ggttcgccaa ccgatacaat
    39421 tttgcgtact tgcttcataa gcatcacgca aaagctgcaa aatagcatct ttcccggaac
    39481 cagcatcaag aactcgccgt tcgcttcttc ccctgaaatg attaactccg gtatcatgtg
    39541 cgccttatgt gattacaacg aaaataaaaa ccatcacacc ccatttaata tcagggaacc
    39601 ggacataacc ccatgagtgc aatagaaaat tttgacgccc atacgccgat gatgcagcag
    39661 tatctcaagc tgaaagccca gcatcccgag atcctgctgt tttaccggat gggtgatttt
    39721 tatgaactgt tttatgacga cgcaaaacgc gcgtcgcaac tgctggatat ttcactgacc
    39781 aaacgcggtg cttcggcggg agagccgatc ccgatggcgg ggattcccta ccatgcggtg
    39841 gaaaactacc tcgccaaact ggtgaatcag ggcgagtccg ttgccatctg cgaacaaatt
    39901 ggcgatccgg cgaccagcaa aggtccggtt gagcgcaaag ttgtgcgtat cgttacgcca
    39961 ggcaccatca gcgatgaagc cctgttgcag gagcgtcagg acaacctgct ggcggctatc
    40021 tggcaggaca gcaaaggttt cggctacgcg acgctggata tcagctccgg gcgttttcgc
    40081 ctgagcgaac cggctgaccg ggaaacgatg gcggcagaac tgcaacgcac taatcctgcg
    40141 gaattgctgt atgcagaaga ttttgctgaa atgtcgttaa ttgaaggccg tcgcggcctg
    40201 cgccgtcgcc cgctgtggga gtttgaaatc gacaccgcgc gccagcagtt gaatctgcaa
    40261 tttgggaccc gcgatctggt cggttttggt gtcgagaacg cgccgcgcgg actttgtgct
    40321 gccggttgtc tgttgcagta tgcgaaagat acccaacgta cgactctgcc gcatattcgt
    40381 tccatcacca tggaacgtga gcaggacagc atcattatgg atgccgcgac gcgtcgtaat
    40441 ctggaaatca cccagaacct ggcgggtggt gcggaaaata cgctggcttc tgtgctcgac
    40501 tgcaccgtca cgccgatggg cagccgtatg ctgaaacgct ggctgcatat gccagtgcgc
    40561 gatacccgcg tgttgcttga gcgccagcaa actattggcg cattgcagga tttcaccgcc
    40621 gggctacagc cggtactgcg tcaggtcggc gacctggaac gtattctggc acgtctggct
    40681 ttacgaactg ctcgcccacg cgatctggcc cgtatgcgcc acgctttcca gcaactgccg
    40741 gagctgcgtg cgcagttaga aaatgtcgat agtgcaccgg tacaggcgct gcgtgagaag
    40801 atgggcgagt ttgccgagct gcgcgatctg ctggagcggg caatcatcga cacaccacca
    40861 gtgctggtac gcgacggtgg tgttatcgca ccgggctata acgaagagct ggatgagtgg
    40921 cgggcgctgg ctgacggcgc gaccgattat ctggagcgtc tggaagtccg cgagcgtgaa
    40981 cgtaccggcc tggacacgct gaaagttggc tttaatgcgg tgcacggcta ctacattcaa
    41041 atcagccgtg ggcaaagcca tctggcaccc atcaactaca tgcgtcgcca gacgctgaaa
    41101 aacgccgagc gctacatcat tccagagcta aaagagtacg aagataaagt tctcacctca
    41161 aaaggcaaag cactggcact ggaaaaacag ctttatgaag agttgttcga cctgctgttg
    41221 ccgcatctgg aagcgttgca acagagcgcg agcgcgctgg cggaactcga cgtgctggtg
    41281 aacctggcgg aacgggccta taccctgaac tacacctgcc cgacctttat tgataaaccg
    41341 ggcattcgca ttaccgaagg ccgccatccg gtggttgaac aggtgctgaa cgagccattt
    41401 atcgccaacc cgctgaatct gtcaccgcag cgccggatgt tgattattac cggcccgaac
    41461 atgggcggta aaagtaccta tatgcgccag accgcactga ttgcgctgat ggcctacatc
    41521 ggcagctatg taccggcaca aaaagtcgag attggaccta tcgatcgcat ctttacccgc
    41581 gtaggcgcgg cagatgacct ggcttccggg cgctcaacct ttatggtgga gatgaccgaa
    41641 accgccaata ttttacataa cgccaccgaa tatagtctgg tattgatgga tgagatcggg
    41701 cgtggaacgt ccacttacga tggtctgtcg ctggcatggg catgcgcgga aaatctggcg
    41761 aataagatta aagcgttgac gctgtttgcc acccactatt tcgagctgac ccagttaccg
    41821 gagaaaatgg aaggcgtcgc caacgtgcat ctcgatgcac tggagcacgg cgacaccatt
    41881 gcctttatgc atagcgtgca ggatggcgcg gcgagcaaaa gctacggcct cgcggttgca
    41941 gctctggcgg gcgtgccaaa agaggttatt aagcgcgcac ggcaaaaact gcgtgagctg
    42001 gaaagcattt cgccgaacgc cgccgctacg caagtggatg gtacgcaaat gtctttgctg
    42061 tcagtgccag aagaaacctc gcctgcggtc gaggcactgg aaaacctcga tccggattca
    42121 ctcaccccgc gtcaggcgct ggagtggatt tatcgcttga agagtctggt gtaattataa
    42181 ttcccggtag aaaaatacta tcgggaatat tatctacagc tgacgaatca aataaaaata
    42241 ccctgtataa taggaaagct tattttacag ggtaaaacca tgccatctac acgctatcaa
    42301 aaaatcaatg cccatcacta tcgccatata tgggtcgttg gtgatattca tggtgaatat
    42361 cagttattac aatcccgctt acatcaactc tcttttttcc ccgaaaccga cttacttatt
    42421 tctatcggcg ataatattga tcgtgggccg gagagtctta acgtcctgcg cctgctaaac
    42481 cagccctggt ttatctccgt taaaggcaac cacgaagcaa tggcgctgga tgcgttcgcg
    42541 accggcgatg gcaatatgtg gcttgccagc ggtggtgact ggtttttcga tttaaatgat
    42601 tcagagcaac aagaggcaat agatctgttg ctgaaattcc atcaccttcc acatattatt
    42661 gaaatcacta acgacaacat aaaatatgtc atcgcacatg cagattatcc ggggagtgaa
    42721 tatctctttg gtaaagaaat agcggagagc gaattactct ggcctgttga tcgtgtgcag
    42781 aaatcgctta atggcgagtt acaacaaata aacggcgctg attattttat atttggacat
    42841 atgatgtttg ataacattca gacgttcgct aaccagattt atattgatac cggatcgccg
    42901 aaaagcgggc ggctgtcatt ttataaaata aagtaatgag gccgggtaaa gcaaagacac
    42961 tacccggcac tttttattag cgcttacctt ccgccagcag cggcgggatg ctcggcacca
    43021 ttggcgcgtc atcaatatct ttctgcgtca tgcggaacgc ttcgggataa tgttcgcggc
    43081 tggtgcggcg tagcggttcg gtatcgcgcc aggtataaag gcaatgctgg cactgatata
    43141 ccgtccagac atctttcacc ggcgatttcg ccatcacttc aatctgttca tcggcacaac
    43201 gtggacaaat catcttcttc tccttattta cgtgcagcca gcatagcggt cagtttttca
    43261 gcccaggctt tggtttccgg caaatccacc accggctggc tgtagtgacc acggttgtcc
    43321 ggggcgacag gcgtggtggc gtcgataatc agcttgtcgg tgatccccgc cgggcttgag
    43381 ccagggtcga gttccagcac ggacatattc ggcaactgca ccaaatcccc tgccgggttc
    43441 actttcgagg agagcgccca catcacctga ggcaggttga acggatcaac gtcttcatcg
    43501 accataatca ccatcttcac gtagcccaga ccgtgcggcg tggtcatcgc acgcaggccc
    43561 accgcgcggg caaagccgcc gtagcgtttt ttggtggaga taatcgccag caggccgtgg
    43621 gtgtacatgg cgtttaccgc ctgcacttcc gggaactcgg ctttcagttg ttgatacagc
    43681 ggcacacagg tggctggccc catcaggtag tcgatttcgg tccacggcat gccgaggtac
    43741 agcgattcga aaatcggcct ggtgcggtaa gagactttat cgatacgcac cacggtcatg
    43801 ttacgcccgc cggagtagtg cccggaaaac tcaccgaacg gcccttcgat ttcgcgtttg
    43861 cggctttcga taaccccttc gaggatcact tctgaacccc acggcacatc aaaaccagtc
    43921 aatggcgcgg tggcgatcgg gtacgggctt tcgcgcagcg cgcctgccat ttcatactca
    43981 gactgatcgt atttcagcgg cgtggccccc atcagcgtga tgatcggatc gttaccgagc
    44041 gtgatggcaa tcggcagatc ttcaccgcgc tcttctgctt tatgcagatg cagggcgata
    44101 tcgtgcatcg gcaccggttg caggccgagc ttacgcttgc ccttcacttc catgcggtag
    44161 atgccgacgt tctgcttgcc gaagttatcc gggtcgagcg gatcgcggga aaccacgcac
    44221 gctttgtcga gatagaaacc gccatcaccg tcgtttaaac gaaacagcgg caggatatcg
    44281 aacagattaa tctcgtcacc atcgacggtg ttctgcgccc aggctggatt ggccggcgct
    44341 ccggggcaat cgggaagtta tcccagcggc ggataaactc atcaatctgc tttttgaccg
    44401 gggtgtttgg cgacaggccg agggaaatcg catgattctg ccaggaaccg atggtgttca
    44461 ttgccacgcg ggcatcggta aagccgcgaa tattatcaaa ccacagcgcg ggcgcgccgt
    44521 cgccgatacg cccggtggcg ttagcagcgg ctgccagatc cggctcagcg ttcacttctt
    44581 cgctgatttt cagtaactgg ccgtggtcat caagcgcctg taaaaagctg cgtaaatcat
    44641 caaatgccat tattcattct cctgagaaaa attccggacc tgcggcaatc cttgccagcg
    44701 cctggcgtgg ggatgttcga ggccaaattg atccagcacg cgggctacca catggtggac
    44761 aatgtcatct atcgtttcgg gatggttata aaaggcaggc atcggcggca ccatcgccac
    44821 gcccatgcgt gaaagtgcga gcatattttc gagatggatg gtgctaagcg gcatttcacg
    44881 cggcaccagc accagtttgc ggccttcttt cagaatgaca tcggcagcgc gccccactag
    44941 cccttcggcg tacccggcgc ggataccggc gagcgttttc atactgcatg gaataacgat
    45001 catgccgtcg gtacgaaagg agccggaaga gatggttgcg gcctgatccg ccgggttatg
    45061 gcagaagtct gcgagggcgg caacatcgcg agcgctgtaa ggcgtttcca gttcaatggt
    45121 ggttttcgcc cacttcgaca tcaccagatg agtctcgaca ttcggcatct cacgtagcgc
    45181 ttgcagtaat gccacaccaa gaggcgctcc ggtggcccct gtcatcccga cgatcagtct
    45241 cattgcttac tctcttaatt gttcgcatac gaacgttatt attaaaatac gcctctgttc
    45301 tcattgcggc aagcactgaa aagtaacaac atccccttcg tcactgaaaa accgctatga
    45361 tagcggcaga tagtttgccc ggagtttaca tggcgttacg aaataaagcg ttccatcagt
    45421 tacgacagct ttttcagcag cacacggctc gctggcagca cgagttacct gacctgacca
    45481 aaccacagta tgcggtgatg cgcgccattg ctgataagcc tggtatcgaa caggtggcac
    45541 tgatagaagc agcagtcagc accaaagcaa cgctggcaga aatgttggca agaatggaga
    45601 atcgtggctt agtcagacgt gaacatgacg ccgctgataa gcggcgacgc tttgtctggc
    45661 tgactgccga aggagaaaag atacttgctg cggcgatacc gattggtgac cgcgtggatg
    45721 aggaattttt ggggcgtttg agtgtagaag agcaggagct gtttatgcag ttggtgcgca
    45781 agatgatgag cacatagaat gctgattgcc gggtaggacg ctgacgtgtc ttatccaggc
    45841 gacaaaaaca caagcttccc aaacaaaaga aaaaggccag cctcgcttga gactggcctt
    45901 tctgacagat gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca
    45961 ggatttcgcg caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga
    46021 ggccaatttc acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc
    46081 gacgtgccag tacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct
    46141 gcttcatatc gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga
    46201 tgtccagcaa cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct
    46261 cgttaagacg aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct
    46321 cttccgcact tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa
    46381 cgttcagctc ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg
    46441 cccgttcaat cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt
    46501 ccgggtcaaa cttctctacc gcgcgaatta gccccaggtt gccctcttcg ataaggtcca
    46561 gcaacgccag accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac
    46621 tctcgatcat ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa
    46681 cttcttcttc ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg
    46741 cgtccaacac acgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat
    46801 cactgggttc ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa
    46861 attccgcatc ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc
    46921 tacccgtgat cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg
    46981 cggcaaataa cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg
    47041 tgttgaactg gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg
    47101 ttgttcccgg accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg
    47161 tttgatgata atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc
    47221 atctgcggtc gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc
    47281 cccctcagaa gcaccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga
    47341 gataggcgta ctggttgatg tactgctgac agtcggctcg gttgtgcttg ctgttggtac
    47401 cgttacaggc gctgtgaccg tggtcgcagt tggcttgttg ttcggcaaca ttttgttagc
    47461 actctgttca cccgaagact cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt
    47521 ggaattttgt gcaggcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc
    47581 atttccgcca gtgattggcg taccggaagc attacccacc tgtaaggtct gaccgacgtt
    47641 cagcgcgtat ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt
    47701 aatccaggcg atatagaaaa gcgtgtcgcc ttttttcacg gtataggtac tgccgctata
    47761 actgcctttc ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg
    47821 taccggctgc tgagctactg gctgcactgg ctggatttgc ggttgttgag tagcctgaat
    47881 ttgtggctgc tgcaccggct gaatttgcgg ttgctgcgct gtagacgtcg tccccatttt
    47941 cggcggcggc ttaatcaaca taccagaatt ggtatttgca ggcgcattgc cattaacgga
    48001 gctgaccggt gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag
    48061 tgacaaagcc gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc
    48121 aggaaaaatt ggttaataac cagtgacata attaccgtgc aaggcaccct actgaacact
    48181 ggaaaagatg ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa tccaggtatt
    48241 tcctcacgtt ttaagccagc tcacccttca ctaaagggac aaagcgcacg gcctccacgg
    48301 tatcgataat aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc
    48361 ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt
    48421 ccggcggtgc cgccgtaaca atgatagcgt caaacggcgc acgtgcctgc caaccttgcc
    48481 atccatcgcc atgacgggtt gaaacattat gtaaatcaag atttttcaga cggcgacgcg
    48541 cctgccactg caaaccttta atccgttcga ccgagcaaac atgctggaca agatgcgcca
    48601 ggattgccgt ttgatatccc gaaccggtgc caatttccag cacccgcgac tgcggcgtca
    48661 gctcgagtaa ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac
    48721 ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt
    48781 cacgcggcac ggcagcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta
    48841 attgatccag aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt
    48901 taaccagtct gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt
    48961 gatagagaca tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt
    49021 accgcccggc gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac
    49081 ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa
    49141 gggtaaatcc ggaacgttaa tattaagaat acgcccggtg cgcagcggct ctttacacag
    49201 tgcgcgcaaa attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag
    49261 cgagacggca agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc
    49321 ggaataaata acatcatccc ccagattcgg cccggcgtta attccggaca caacaatgtc
    49381 cgggcgcgga cgcatcagag cattcacgcc aagatagacg caatcggtcg gggttcccat
    49441 ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag
    49501 agaatttgac gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc
    49561 acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag
    49621 caatatgcgc ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac
    49681 tacccgccgg aagccagaaa cggatctcta cggtgacgtc atcccaccaa ttccagctta
    49741 attgttgcgg atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta
    49801 aagcttgtaa ttcagtttct gcggcgacag ctgcttgttc gaatgccagc gcttcacgct
    49861 gagttcccca ttcgccactg cccggcaatg cggcggttat catcagctct ttatcgttga
    49921 cgcgacgctg taattccgcc agttcttcgg tggttgctac aaaccagcta ccacgtccgg
    49981 ctaattgtag cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag
    50041 caacaatctg attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc
    50101 gatcgcgcac cggagtattg gtttgcgccc agcgcagcgc gccctgcaag ttgctaccgc
    50161 caatcccaaa acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgatca
    50221 gacgttgttc aacgtcatcg cgattgctca cttcgcgcag aaccagggta aaggcgtttc
    50281 ctttcagcgc ccctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc
    50341 cttccagttg aaaggcgctc agatcgggca tttccttgcc cggcacgcga gcgcataacc
    50401 actgttccgt aacagcatgt ttgtcttttt gcccagcgaa gctgacttca cgggcatgaa
    50461 ttttcaggaa tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga
    50521 ttctaaccag aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca
    50581 caaagtcttc cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag
    50641 tgagattatc aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc
    50701 aatcccttcc ccacgtccgg taaatccaag tttttccgta gtagtggctt tcacgttaac
    50761 atcatccata tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg
    50821 caacatcttc ggtgcctgag cgatgatagt gacatcgacg ttgccaaggg tataaccctt
    50881 cgcctgaata cgacgccagg cttcgcgtag cagctcgcgg ctatcggcac ctttaaatgc
    50941 cggatcggta tccgggaaca gtttgccgat atcccccagc gccgccgcgc caagcaatgc
    51001 atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta
    51061 aggaatgcgt acgccaccaa tgataattgg gccttcaccg ccaaaggcgt gtacgtcaaa
    51121 accgtgtcca attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg
    51181 gccagtgcca aatcttccgg ccgcgtgact ttaatgttat ccgcgcggcc ttcgaccaac
    51241 tgcggatgaa atccgcaata ttccagcgcc gaggcttcgt cggtaatagt cgcgccttca
    51301 tttagagcgc gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc
    51361 gcgtgccata agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca
    51421 cgtttcatag tatcgcgcac tggtgcggcg aggatccctc ccgtgcggct ggtttcgctc
    51481 aacgccaaca atcgcgcgag gtcatcctga tgcagacaag gacgagcggc gtcatgcacc
    51541 aatacccact gcgcgtcgcc agcggctttc aaacctgcca gcacggaatc ggcacgctca
    51601 tcaccgccat ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg
    51661 ctatcgccag gacttatggc aatgacgaca cgtttcaccc ggggatgcgc cagcagcgca
    51721 tgcaccgagt gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc
    51781 gtttgcattc gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg
    51841 gttgccatgt taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc
    51901 ttcgacgcgt caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca
    51961 ttacgcgcac gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa
    52021 agttgatcgt ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatca
    52081 ttgacgcggg tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag
    52141 accagaatag ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat
    52201 catcatccca tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc
    52261 gggcaaatgt accacatttg tccattgtta cgtataccca gggcgtgcag aacataatct
    52321 cattattagt tacggtttga attatgaaca gaggagacga gaaagtacaa attagcccag
    52381 tagccacata aacagtgcgc caaacataat gcctactgtc accagggtga aaacaatact
    52441 gtagcgtagc tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcgacggg
    52501 catcagcgcc agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata
    52561 aatcaacatc ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg
    52621 cagtgaccat gtggtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt
    52681 atggctatta cgcatatttg atcctgttac tttgacgaac cgggcatgga aacccggtgg
    52741 tgtctcagga tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat
    52801 ttgttactaa ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg
    52861 aatctattcc cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagccttttg
    52921 gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt
    52981 ctcctacgcg ttcacgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca
    53041 gcaccaccag tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct
    53101 ctttacgatc ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattgtcgc
    53161 catccagcag atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga
    53221 ccgttgattt accggacccg gagaggccgg taaaccacag cactacacca cgatgaccgt
    53281 ggtgtagctc gcgttgttgc acagtgaccg gatggctatg ccagacgacg ttttcgtcat
    53341 gcagcgccat tatttatccc ccagcaaatc gcgcgcgccc cagtgtggaa agtggcggcg
    53401 aaccagggca ttcaattcca gttcgaatgc actaaattca gatggcgcag cagttgcctg
    53461 gctaactggc tcatgcacca taccggcacc tacggtcaca ttgctcaggc gatcgataaa
    53521 aatcagccca ccggtaaccg ggttttgctg ataacgatct aacaccagtg gctcgtcaaa
    53581 agtgagatcc accaggccga tgccgttcag cggcaggttt tcaacttcgc gttgggtaag
    53641 gttattaata tcaacctgat aacgaatgcc atcaacacga gcacgcgtct tcttaccggc
    53701 aattttgatg tcataactct ggcccgggga aagcggctgt tccgccatcc ataccacatc
    53761 caccgacgcg ctctgcacag ctggtaacgc ttcgtctgcc gccagcagca gatcgccacg
    53821 gctgatgtcg atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc
    53881 gcgatcacca tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcac
    53941 ttttacacgt tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa
    54001 atcgagattt gggcggttaa cgtactgcac cgggaagcgc attggctggg catccaccac
    54061 tcgctggatc tccacggttt ccagcacttc gagcagtgtc ggaccgctgt accacggcat
    54121 actttcactt tgcgaagcca cgttgtcacc ttccagtgcg gagagcggca caaagcggat
    54181 atccagatta cccggcagct gcccggcaaa ggtcagataa tcttcacgaa tacgggtgaa
    54241 cgtctcttca ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat
    54301 ccccaacagt gtggagataa aactgtgacg acgggtttga tcgagcacgc ctttacgggc
    54361 atcgatcagt aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgggtgta
    54421 ctgctcgtgc cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaataacg
    54481 gtaagccacg tcaatggtga tgccctgttc gcgctcagct tgcaggccgt ccactagcag
    54541 tgccagatcc agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga
    54601 gagctgatct tcgtagattt ggcgggtatc gtgcagcagg cgaccaatca gggtactttt
    54661 gccgtcatcg acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat
    54721 catccaggct tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt
    54781 aaaaataccc ctgacgtttt ttcagctcca tggatcccgc ctggtcgcgg tcaatcacgc
    54841 gaccctgacg ttcactggtg gtggaaacca gcatctcttc gatgatttcc ggcagtgttt
    54901 gtgcatttga ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca
    54961 tccgtttttt aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca
    55021 acataccgtc gcgttccaga accggacgtt ccgcagcgag atacagcggc acaatgtcga
    55081 tattttccag ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaga
    55141 cacggatgct ttcgccttta ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct
    55201 gatttttcgg atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc
    55261 gggatttctc ttcgtcgcgg cgcgcaccac cgaaggcggc atcaaaaccg tatttattca
    55321 gcgcctgttt caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgcacga
    55381 atggattaat ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt
    55441 aggctttcgc cgtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcaa
    55501 catgcagcaa cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca
    55561 tgacgctgga atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca
    55621 cctcgcgaat aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgttttt
    55681 gatccataac cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata
    55741 gggagcgtaa gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa
    55801 cgatttggca aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa
    55861 gatttgaaat tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat
    55921 ggtatttaag gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc
    55981 ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat
    56041 actcaggcac gacatattgc tactttcttt ccgggccgca tgaccggaac tcctgcagaa
    56101 atgttatctg ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt
    56161 cggacattta atagtcggta tatttatacc gcccgcgata atcgcaagag ctggcataac
    56221 gtgacgggaa gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt
    56281 atggcgcatc tggatactta cgccccgcta agcgatgctg acgccgatgc caatctcggc
    56341 gggctgacgt tacaaggaat agatgataac gccgcaggtt taggtgtcat gctggaactg
    56401 gcagaacgtc tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc
    56461 gaagaggaag ggaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag
    56521 aaaaatacgc tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc
    56581 aacagcggtg taaaaacccc tgaagcagta aggaaattaa cgcgcgacag agcgctggca
    56641 attgcgcgta gtcatggaat agccgcaacg accaatccgg gtttgaataa aaattatccg
    56701 aaaggcactg ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt
    56761 tcggtggaag cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa
    56821 acacctgcct tcccggcggg aaatagctgg catgacgtaa gactggataa tcaacaacat
    56881 attgataagg cacttcctgg aagaatagaa cgtcgctgcc gtgatgttat gcggataatg
    56941 ctaccactgg tgaaggagct ggcgaaggcg tcttgatggg tttgaaaatg ggagctggga
    57001 gttctaccgc aggggcgggg aattctcagt gatatccatc atcgcatcca gtgcgcccgg
    57061 tttatccccg ctggcgcggg gaacacgccc acgcctcaac gcggcacata aaatcgtgcg
    57121 gtttatcccc gctggcgcgg ggaacactat tcgagcacca gaaagcatca ctggacgccc
    57181 ggtttatccc cgctggcgcg gggaacacta acagactgaa atctgtaacg gcgacacgta
    57241 cggtttatcc ccgctggcgc ggggaacact ccgacatttg actcgatgcg cgaggggttt
    57301 acggtttatc cccgctggcg cggggaacac ccatgttaat gatgctcttc ctcctagccc
    57361 aacggtttat ccccgctggc gcggggaaca ctacaaacgg tgggtgcgtg cggaagtcgt
    57421 aatcggttta tccccgctgg cgcggggaac acatctggtt tgtcacctcg gtgccgtagc
    57481 cgttcggttt atccccgctg gcgcggggaa caccacattc aacaggttaa ggtaaccgat
    57541 ttgaccggtt tatccccgct ggcgcgggga acacactaag catacatatc tgtttttaaa
    57601 caaatttatt ccacatcaac aatctaccaa ctaaattcaa acatttcctt atttttaaag
    57661 aacacataac ctattgatta tcaacaggaa gaaaagaaac caaacgtaac ccatccaaat
    57721 ccaccggaat acgtctgttt tctccccagg tctgaaattc aaaacccgac tcggtattgg
    57781 tcgcccaggc catcaccaca tttccgcaac cagccagttg ggtaatttgc tgccagatca
    57841 tctcccgaat acgttttgat gtatcaccaa catacacacc ggcacgcact tccagtagcc
    57901 agattgcgag ccgtccacgt aagcgcggag ggacattttc tgtaacaacc acgaccatgc
    57961 tcatccgccg cgcccccggt gaccactatc acccagcgtt tcaggttcag ggatggcagg
    58021 cggtaacata tccggcgcgg gttgtggtgg ttcaatttca cctgcagcaa ggacttcctc
    58081 aattaacggt attaatttgc ccgttaactt agtgctacgg aaaatatcgc gacaggctaa
    58141 tctgacttct ttatcaggtt ctgcgagttg cctcgctgct atttcaaatg cctttggcac
    58201 aaccgaatca aatttaatga tatcggctat gtcataaaca aatgaaagcg gtttgccact
    58261 atgaataaat ccaatagcgg gcgcatatcc cgcggctaat actgccgctt cagaaatacc
    58321 gtacagacat gatgtggcag cactgatgca gcgattcaca acatcgcctt tttcccagtc
    58381 tttaggatcg tatttgcgac cattccattt cacaccatat tgtttcgcca gtaatgcata
    58441 ggtctggcga acgcgggatc cctcaattcc ccgtagctga tccactgaac ggcgagctgg
    58501 cggtggctca cgaaaacgta attcatacat tttgcgcacc accttcaggc gtagatcttc
    58561 cgttaaagcc agctttgcct ggtagagtaa tttatctgcc cgcgcccctc cgggttgtcc
    58621 ggaagagtaa acgcgaacgc ccgcttcacc gacccagacc agcagtgttc ccaccgtggc
    58681 ggccagatgc accgccgcgt gggaaactct cgttcccggt tcgagcataa tgtaggcgac
    58741 cgatcccacc ggaatgtgcg tgcggatcct ggttttgtcg atcagcacga aagcgccgtc
    58801 cagtacgtcg atttgaccgt actggaggaa gatcatagag gtgcgatctt ttaacgggat
    58861 cggactcagt ggtacaaacg tcacgcctct gctccgggtt tgatcagcat cagaccacag
    58921 ccgaacgcgc ggcttttgcc atacccctgg ctcagacgtt gcagaaataa ccccggatcg
    58981 gtgaccgtaa gcattcccgt ataatccacg ctactgaact ggatcagttg tcgggagttt
    59041 tcccgccgga gctgctgctg tcggtaggca tcgaccgagg tatccagcag tgtaaaacca
    59101 cttctttccc cctgaacagc cagccagtcc agcgccgcct gttgttgatg caaccagaca
    59161 tcacttcctt ccgcctgccc cctcatctgc cgtttcgcct ccatcagcag atcgtggcgc
    59221 ttgcccgctt tacagattgt tggattcgcc cgcaggttga aacaaagttg ttgtccggta
    59281 cgcagctcgg gcacaaatga ccggcattcg atggtgaacg tttcactttc cgccgggcgt
    59341 tcctgcgaca gtacaaaaaa gcgaaacgcg ccctggagtt cttcccgacg ataaagaaat
    59401 tgcctttctt tgccgccagg gaagagatcc cacagccact gatgcatcac atattccccg
    59461 cgatccacca gatgcagcaa ctgcgcaggc gaaagctggc cggtatgtaa ggttattctt
    59521 gagaggtaca tggttcctcc ttgctgagcc acggcccctg attgacggtg cgttccccaa
    59581 acagccattg ctgacgattt aaaggaacat cccggcggcg caatattttg ctcgcgacca
    59641 ggccgtcgtg ctccccttcc caccagcatt catcctgaag tttcgggagt gcgattttca
    59701 ggtcgtggaa atgctcctga tactggtgat atgcgttacg taaaacatca gacgcgttgc
    59761 cttcgagcag taacggcgca agtggtaatg ccagcggatg actttttcgc cccagataaa
    59821 gaggaaaaac tggatggcgt aaaccgccct gcaactgttc gaggctgtaa ggcgcatcgg
    59881 ctgttgttgc caccgccacc atccaccagg cgtcggtgta gtagtcgcgc cgggagataa
    59941 tcgcgctcag aagatcaggg tcgctcaact cttcgcgacg gctgaaataa cgcgctttac
    60001 gcacctcttt tggcatctgg atcgtgtgat aatcccgtgc ccagcgcggg ttacggctgg
    60061 cgcaaaccac cagtgaatag tggcgattaa acgcgtttaa gcgttcggta tcatcacgcc
    60121 gaatccctac cccggcagcc agcagaccca gcaatgctga gcgcgaaggc agttcatggg
    60181 tatgacgcac ttcgccgggg gcatcgacgc cccaggatgc cattggccca tgaagctgaa
    60241 aaatcagata ttggctcata agccgcccct tacgcgcaga taaagtccag cacgtccttc
    60301 atgcttccct gtttgttcat cacgtcaaaa cttgcgcatt cggtcttctg ttcgtagacc
    60361 gtattcatat tttcgcgaag cgttgtaata agctgcaccg ccacgtctaa ctgccgggtg
    60421 ccattaatgg gttcatagaa ggccgcagcc agagaacgcg gttgatcggt gcctttttcc
    60481 gccagcgccc aggaagcata ggcacggctg gcaaagctat tttgtttacc ggttggggaa
    60541 actttaagcg cggcttccgt aaaagcgcgt atggtctgat tagctaacgc ttcgtcaccg
    60601 ccgaggtttt ctaccagcag atctttatca atgcagatat aggtgtagaa aagtgctgaa
    60661 ccgaatccgg tttctccgag atgcccggca ccggcatctt cagaggcctg gcgcaaatca
    60721 tcaacggcgg tgaaaaaatc atcttcgaca atcgtttcac tgacaccaaa cgcatgcgcg
    60781 acctggcagg cggcttcaac attaaactca ggtttattcg ccagcatacg accaaacata
    60841 gcgatatcta ccgctatacg gtctttacgt aacaacgcga gatcttcctc ttttggcgcg
    60901 cgcttttctt ctgccagttg atgggccagc gcttttacgg cgtcaaattc tgccgggctg
    60961 atatgaacta attgttcagt ttcggcgtta gtgagcggat cttttggttt tttgtcgttt
    61021 ttagctttcc caagataatc cgcaattttt gccgcccatt cgatggcttt tttctcttcg
    61081 atgcctttct caatcaggat agttgccgcc tcacgcgcaa ttcgcccact gcgaatacca
    61141 atatggcccg ccagtgcctg ttcaaaaagt gcagaagtgc gccacgcacg tttcagactt
    61201 tgtgaggaaa cgcgcagtcg cgttgctcca cccaggacca cggttttcgg cgctccggtg
    61261 tcatcacggt taaggttggc agcagggtaa gcggttaaca aatgaagctg aataaatgtt
    61321 gtcatgagat agtcctttat taattttgtt cgttatcggc gtcgccagcc tggtaatatt
    61381 ccagtgccca gcggatacgg ataaattcgg ttggccgctg ctggcggcgg tgatgattga
    61441 gcaggtcgtc gctctcctgg caccagcgga aaatatcttc tgccaaagag ataagattga
    61501 cggaaccgtt taataatttg accgcgcgac gtaactgacg cagtaaatca tccggcgttt
    61561 ttactgccga caggcgagta aaacgcccct ccgacattac cgccgccagc tgcgcggcaa
    61621 aaggctgccg ttcatcaatg gcttttacat tggcgctaac tgcggcgacc agcgccagtg
    61681 cggtaaaacg ccactcttgt gcttcaattt tccacaaagt gtgggtctgc attagcagag
    61741 aacgaaaccc ttctgacaag caaacatcat taaccgttgt gctacgccgc agactggcac
    61801 gattgccgcg tttagcctgc aactcttcgt gccatttacg cagcgtggct ttgtgctcct
    61861 cttttacaat actcattcag cagcctcctg ctttttctcc ctggcggctt tagcattttg
    61921 cttctccgcc gatgttgtaa aatatttctt gcgtgcggtc atgacgcgtt tcaaatcaac
    61981 gggctcataa ggattggtga atacacgctc gtcaaaatcc tgacgcgcaa ataaccaaat
    62041 ttccttttgc catttgccga gtaattcatc tggatcctga ccttcttcaa tttggcgcac
    62101 taacctcagg aagcgatgct gagttttgtt ccagaagtcg atatccacaa aactaaaatc
    62161 accccttgca ccttttggat cagagaacca tgcttctttc aatgcactcc gtaacagact
    62221 tagaatccgt gaagccgttt gcgcagccag ccgcagcttt ggtatctggc cttctttttt
    62281 attgagcaat agagggaaat ggtgttcgta ccaacagcga accttcatat cttcgaaatc
    62341 ataaccaaat ccccacaagc ctatttttgt ctgctttaga ttgctggcat tgaaaagctt
    62401 cactacctgt gcggggagtt cagtattgtt ttttgattta ccttcctcta tgaggcctaa
    62461 ccagtcgcgc cagattaaac cacctggttg tggtttaact gagtaaaact ctcctccctc
    62521 tttaagcggc atacggtaag gtgttagagg atgcctccac atgacgtatt gcaccccatt
    62581 gtttttaagt gccatcaagc tcaatagagc gccgctctga ctatcgcaaa tatcgcagtt
    62641 gccaattgag gttgtcttaa aatcaatacg aatacgccga ggcattcccc agtaggcctg
    62701 aagtttattg acttgttcat cggttatcac ggcaccatcc agttcacagg tacgcgtcgg
    62761 tgcaagccag ggaaagacca ggtcatcaaa ggtttttggt aaaggtaaat cggcttcatc
    62821 ctgtggcatt acgttaagcc acagcttgcg ccacaaaggg ggttgtggat tgccctgata
    62881 ctccagcaat tctataagtg tggtcagcgg ccccccgcca cgtaaccccg gatagtaacc
    62941 gcgtccacct ttgggagcat tcagctggat tgaaaaaagt gctaaaggca cacaatgcag
    63001 gcatatatgc tgaattgtac ctcgcttaat aaaatggtct ttatttcgtt cttttgtttg
    63061 ttttcctggt gtgtcaggaa gaagcgaagc aatagaacta accttaactc ttaattcttc
    63121 gaaatcctgc ataaatgaag gtgaatctgg cccgaactgg aaagcatgtt ctaataacag
    63181 caatgcttcc cgtagctttt cggcttccag tccgtcttcc cagatatcat cccaacgacg
    63241 atgatctttt ggcgcgaagc tggtttgtag taaccccagc aaaaactgcc aggccgcccc
    63301 ctggagatct gcccgcggcg cagcgatatc gacaacattt tcatccgcca gatcgactgg
    63361 cgccagtttg cctgtcgttc cgtctttaaa acgaacgggc aaccacgggg ttgtcagaag
    63421 tgaaaacgag ttcatacgca tcctctcgta aaaaagacca tccaggtcgg ttatccatcc
    63481 ttttcggtgt atcaactatt cagagagtct ctgtcgcagg catctcaatc aaccttgcca
    63541 atcaatccct ccctggccga atacccgcaa ctttcgtcat cagtgactaa aatcacgttt
    63601 gccatctccg gatcttgccg ttgttcaaca caccactgcc tgaacgcttc cccttccagt
    63661 aaagaaaatt catcccgatg ttttttccac cagcttcgac gcactctggc aacgctcatt
    63721 tcccatgcgt gagcaccgct ggcataaggc ttcaccacac cgtcaataca ggtagccagc
    63781 cacagggaaa cagactcttc agccagacgt gtcgacagct tttccgggag gtaatcgttg
    63841 atattggcgg catagccggg cctgaagttc agcataaact ttttagccat tgcgcgatcg
    63901 cagtaatatt tgcccacttg ctcctgctcg ctgcgggcaa atccttccgg cattaccacg
    63961 tcctcaccgt agacggattc aataagaagc cgggctgcgt gtggcatctg aatagcgcct
    64021 tgctcacgca atactcgctg cgtcagccag attcgcccat gatccgggta gacatatgca
    64081 ctgttacgca tggcactgcc gaaccattcg tcaccaggag cgtcgtccca gacgggggcc
    64141 agaatcagca attcaggagg ggaacgctcg tcttttccgt cacgctttaa ctgaccatta
    64201 atatcgcgga tatgtcgctg taatcgcccc gctcgctgga tcagcaaatc aatgggggca
    64261 aggtcggaga tcatttcgtc caggtcaata tccacacttt gctcaattac ttgtgtacaa
    64321 acaatgactt gcgatgatct ttttaaatta caatttttac caaagcgtgc cagcgtttcc
    64381 gtttcgattc gctggcgatc gctaaaagca aagcggctat gaaagagtga aaggctggaa
    64441 gcgggaatga cgccgcgggc aagcagctga cgatgaacct taatagcgtc atcgacagaa
    64501 ttgcggatcc aggcgatgca ttttccctga cttaccgccg attcgatacg cgcaatactc
    64561 tcttgttcac tatgaagcca acccacgctg acgctacgct caacgtcttt gcgcgtcgct
    64621 acccggtgtg agttcacatc ggatttcgtg acatgcgtca gccaggggta atcatccttt
    64681 tcaaggaacg gagcttcttg ctggccctct gtgccacgcg caaaggcggc gacaagtttg
    64741 tcgcgctgct gttgggataa cgtagcagaa agcaaaatga cgctgtttcc accacgcgcc
    64801 tgccgctcga tcagcccttc aagaatgcat gacatgtaag cgtcacaggc gtggatctca
    64861 tcagccagca ggattttgtt actcagcccc agaagccgca gattattatg tttgaacggc
    64921 atcaccgcca tcatcgcctg atccagcgtg ccgacgccaa tttcagccag taaagccttc
    64981 ttgttactgt cggcaaacca ggctgcacat ccttgactga acgcttgttc atccggttct
    65041 tctgacccga ctaaatcacc ggaccagagt gattcattaa agcggtccat taatgtgcgg
    65101 gcactgtgtg tcagcaccag gctggggcgg gactctggcg aatagaaagc aagccaggtt
    65161 ttgaccagcc gatcgtacat ggcattggcc gttgccattg ttggcaggcc aaaaaacaaa
    65221 ccctgtgctt tcctcgcagc catcaacctg tgcgccagga taagcgccgc ttctgtttta
    65281 cctgcgccag tcacgtcttc cagaataaat aactgtggcc ctggctggct gatatccaga
    65341 tccagcacct tttgctgtaa tggtgtcggg tgctcaataa aaggaaacag cgtattaact
    65401 ccggtgaaag gtgcggtttc tgcttttgga ggaaagacgg ttaaggcgtt ttgagcctga
    65461 actaaagttt tctgccagta atctttaata tccattgggt gtgcgacgcg tggaaaaaat
    65521 cgcgttgacg aacccgtcca gtctgcgagt acgactgttg cagagatata ccaggaaagt
    65581 tgttttaaaa gttcaacgcc ctcgtcatta tcccagaatg tgggaatctc tatgagcgga
    65641 cacagtgcct tgatttcaag gagaaaatct cgcgcggcaa ctttgtcttc aggcagaaaa
    65701 ttatccagtt catcaatacg gtcagggggc cgaccatgat gcccggtagt catgggcatc
    65761 cacatcgcta ttacacgttt aagtttgcga gtagagagtg aagaggcagg aagcaattct
    65821 tcgcattcac ttaaataatg atgccacaac cagtagccca gcgttgaatg agagatcttt
    65881 tcgtaattct ttcttgcgcc ttccggaacc ttgagttgtg ggtgcaggta aagttgctga
    65941 aatgagcggg caaattttcc aatatcgtgc cagcacaaca accaggcgaa aaattgagcc
    66001 gcctgttcct tgtcagaaat ccctaattga cgaaaataat cagccagccc gaagcaattt
    66061 cttttaacca ttaaatagcc catcgcggcc acatccagcg aatgccagca aagaaggtga
    66121 tagtcgtcac ccccttcttt ctcgtcgcgt cgggtttttc cccagaaatc aaagaaagtc
    66181 acaatatttt tatccttcag taaacttaaa ggatatttac gcatatttta aaaattatct
    66241 gtgatatata tcagccaata aacatttata ttatgaataa attaatg
//
LOCUS       DAPGRN010000012        65352 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPGRN010000012 DAPGRN010000000
VERSION     DAPGRN010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.012"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(187..666)
                     /gene="folK"
                     /locus_tag="RHW33_000863"
     CDS             complement(187..666)
                     /gene="folK"
                     /locus_tag="RHW33_000863"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV1644404.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILTVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGETLREILTSLAVPQPAIW"
     gene            complement(663..2081)
                     /gene="pcnB"
                     /locus_tag="RHW33_000864"
     CDS             complement(663..2081)
                     /gene="pcnB"
                     /locus_tag="RHW33_000864"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV1644405.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2120..3016)
                     /gene="gluQRS"
                     /locus_tag="RHW33_000865"
     CDS             complement(2120..3016)
                     /gene="gluQRS"
                     /locus_tag="RHW33_000865"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV1644406.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAHARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLLEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDLLRGSIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3083..3538)
                     /gene="dksA"
                     /locus_tag="RHW33_000866"
     CDS             complement(3083..3538)
                     /gene="dksA"
                     /locus_tag="RHW33_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV1644407.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3716..4420)
                     /gene="sfsA"
                     /locus_tag="RHW33_000867"
     CDS             complement(3716..4420)
                     /gene="sfsA"
                     /locus_tag="RHW33_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV1644408.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4435..4965)
                     /gene="thpR"
                     /locus_tag="RHW33_000868"
     CDS             complement(4435..4965)
                     /gene="thpR"
                     /locus_tag="RHW33_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV1644409.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPLEAGRPVAADNLHL
                     TLAFLGDVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5039..7468
                     /gene="hrpB"
                     /locus_tag="RHW33_000869"
     CDS             5039..7468
                     /gene="hrpB"
                     /locus_tag="RHW33_000869"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV1644410.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGVFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTLKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAGKWLPEYDWPAVDDESLLATLETWLLPHMAGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7663..10188
                     /gene="mrcB"
                     /locus_tag="RHW33_000870"
     CDS             7663..10188
                     /gene="mrcB"
                     /locus_tag="RHW33_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV1644411.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDYDDYDDYEEEEP
                     MPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPAA
                     VYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFPD
                     SKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSGF
                     PDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLS
                     SERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGR
                     PVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDMLS
                     ARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKA
                     AVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGSL
                     AKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDAL
                     TRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQT
                     IASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGRQ
                     LGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSIY
                     QRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQQ
                     QPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10408..12651
                     /gene="fhuA"
                     /locus_tag="RHW33_000871"
     CDS             10408..12651
                     /gene="fhuA"
                     /locus_tag="RHW33_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV1644412.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLDLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAATTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12702..13499
                     /gene="fhuC"
                     /locus_tag="RHW33_000872"
     CDS             12702..13499
                     /gene="fhuC"
                     /locus_tag="RHW33_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV1644413.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13499..14389
                     /gene="fhuD"
                     /locus_tag="RHW33_000873"
     CDS             13499..14389
                     /gene="fhuD"
                     /locus_tag="RHW33_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV1644414.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14386..16368
                     /gene="fhuB"
                     /locus_tag="RHW33_000874"
     CDS             14386..16368
                     /gene="fhuB"
                     /locus_tag="RHW33_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV1644415.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGVALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16403..17683)
                     /gene="hemL"
                     /locus_tag="RHW33_000875"
     CDS             complement(16403..17683)
                     /gene="hemL"
                     /locus_tag="RHW33_000875"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV1644416.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17908..19329
                     /gene="clcA"
                     /locus_tag="RHW33_000876"
     CDS             17908..19329
                     /gene="clcA"
                     /locus_tag="RHW33_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV1644417.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19353..19418
                     /locus_tag="RHW33_000877"
     CDS             19353..19418
                     /locus_tag="RHW33_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644418.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19411..19755
                     /gene="erpA"
                     /locus_tag="RHW33_000878"
     CDS             19411..19755
                     /gene="erpA"
                     /locus_tag="RHW33_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV1644419.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19802..20425)
                     /locus_tag="RHW33_000879"
     CDS             complement(19802..20425)
                     /locus_tag="RHW33_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV1644420.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20463..21263)
                     /gene="btuF"
                     /locus_tag="RHW33_000880"
     CDS             complement(20463..21263)
                     /gene="btuF"
                     /locus_tag="RHW33_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV1644421.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTTGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21256..21954)
                     /gene="mtnN"
                     /locus_tag="RHW33_000881"
     CDS             complement(21256..21954)
                     /gene="mtnN"
                     /locus_tag="RHW33_000881"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV1644422.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22038..23555
                     /gene="dgt"
                     /locus_tag="RHW33_000882"
     CDS             22038..23555
                     /gene="dgt"
                     /locus_tag="RHW33_000882"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV1644423.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPTIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23685..25109
                     /gene="degP"
                     /locus_tag="RHW33_000883"
     CDS             23685..25109
                     /gene="degP"
                     /locus_tag="RHW33_000883"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV1644424.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25264..26421
                     /gene="cdaR"
                     /locus_tag="RHW33_000884"
     CDS             25264..26421
                     /gene="cdaR"
                     /locus_tag="RHW33_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV1644425.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26510..26896)
                     /locus_tag="RHW33_000885"
     CDS             complement(26510..26896)
                     /locus_tag="RHW33_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV1644426.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27210..28034)
                     /gene="dapD"
                     /locus_tag="RHW33_000886"
     CDS             complement(27210..28034)
                     /gene="dapD"
                     /locus_tag="RHW33_000886"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV1644427.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEVRFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28065..30737)
                     /gene="glnD"
                     /locus_tag="RHW33_000887"
     CDS             complement(28065..30737)
                     /gene="glnD"
                     /locus_tag="RHW33_000887"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV1644428.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECILEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSPELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30799..31593)
                     /gene="map"
                     /locus_tag="RHW33_000888"
     CDS             complement(30799..31593)
                     /gene="map"
                     /locus_tag="RHW33_000888"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV1644429.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31961..32686
                     /gene="rpsB"
                     /locus_tag="RHW33_000889"
     CDS             31961..32686
                     /gene="rpsB"
                     /locus_tag="RHW33_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV1644430.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32821..33672
                     /gene="tsf"
                     /locus_tag="RHW33_000890"
     CDS             32821..33672
                     /gene="tsf"
                     /locus_tag="RHW33_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV1644431.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33819..34544
                     /gene="pyrH"
                     /locus_tag="RHW33_000891"
     CDS             33819..34544
                     /gene="pyrH"
                     /locus_tag="RHW33_000891"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV1644432.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34694..35251
                     /gene="frr"
                     /locus_tag="RHW33_000892"
     CDS             34694..35251
                     /gene="frr"
                     /locus_tag="RHW33_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV1644433.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35343..36539
                     /gene="ispC"
                     /locus_tag="RHW33_000893"
     CDS             35343..36539
                     /gene="ispC"
                     /locus_tag="RHW33_000893"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV1644434.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36728..37486
                     /gene="ispU"
                     /locus_tag="RHW33_000894"
                     /gene_synonym="uppS"
     CDS             36728..37486
                     /gene="ispU"
                     /locus_tag="RHW33_000894"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV1644435.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37499..38356
                     /gene="cdsA"
                     /locus_tag="RHW33_000895"
     CDS             37499..38356
                     /gene="cdsA"
                     /locus_tag="RHW33_000895"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV1644436.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38368..39720
                     /gene="rseP"
                     /locus_tag="RHW33_000896"
     CDS             38368..39720
                     /gene="rseP"
                     /locus_tag="RHW33_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV1644437.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39750..42182
                     /gene="bamA"
                     /locus_tag="RHW33_000897"
     CDS             39750..42182
                     /gene="bamA"
                     /locus_tag="RHW33_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV1644438.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42304..42789
                     /gene="skp"
                     /locus_tag="RHW33_000898"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42304..42789
                     /gene="skp"
                     /locus_tag="RHW33_000898"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV1644439.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42793..43818
                     /gene="lpxD"
                     /locus_tag="RHW33_000899"
     CDS             42793..43818
                     /gene="lpxD"
                     /locus_tag="RHW33_000899"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV1644440.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43923..44378
                     /gene="fabZ"
                     /locus_tag="RHW33_000900"
     CDS             43923..44378
                     /gene="fabZ"
                     /locus_tag="RHW33_000900"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV1644441.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44382..45170
                     /gene="lpxA"
                     /locus_tag="RHW33_000901"
     CDS             44382..45170
                     /gene="lpxA"
                     /locus_tag="RHW33_000901"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV1644442.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDLAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45170..46318
                     /gene="lpxB"
                     /locus_tag="RHW33_000902"
     CDS             45170..46318
                     /gene="lpxB"
                     /locus_tag="RHW33_000902"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV1644443.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46315..46911
                     /gene="rnhB"
                     /locus_tag="RHW33_000903"
     CDS             46315..46911
                     /gene="rnhB"
                     /locus_tag="RHW33_000903"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV1644444.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46948..50430
                     /gene="dnaE"
                     /locus_tag="RHW33_000904"
     CDS             46948..50430
                     /gene="dnaE"
                     /locus_tag="RHW33_000904"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV1644445.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50443..51402
                     /gene="accA"
                     /locus_tag="RHW33_000905"
     CDS             50443..51402
                     /gene="accA"
                     /locus_tag="RHW33_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV1644446.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51501..53642
                     /gene="ldcC"
                     /locus_tag="RHW33_000906"
     CDS             51501..53642
                     /gene="ldcC"
                     /locus_tag="RHW33_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV1644447.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEEWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53699..54088
                     /locus_tag="RHW33_000907"
     CDS             53699..54088
                     /locus_tag="RHW33_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1644448.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54153..55448
                     /gene="tilS"
                     /locus_tag="RHW33_000908"
     CDS             54153..55448
                     /gene="tilS"
                     /locus_tag="RHW33_000908"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV1644449.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVAL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQVFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLLWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAETTARSAALCAE
                     QESLLDELLADNLAHCQSPQGTLQIVPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSENIVPWQTWLQPLELPA
                     GLGSVQLNAGGAIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGISFVWQRNA"
     gene            complement(55501..55761)
                     /gene="rof"
                     /locus_tag="RHW33_000909"
     CDS             complement(55501..55761)
                     /gene="rof"
                     /locus_tag="RHW33_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV1644450.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55748..55948)
                     /locus_tag="RHW33_000910"
     CDS             complement(55748..55948)
                     /locus_tag="RHW33_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV1644451.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56114..56659
                     /locus_tag="RHW33_000911"
     CDS             56114..56659
                     /locus_tag="RHW33_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV1644452.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56656..57078
                     /gene="arfB"
                     /locus_tag="RHW33_000912"
     CDS             56656..57078
                     /gene="arfB"
                     /locus_tag="RHW33_000912"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV1644453.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKEHLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKELTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57092..57802
                     /gene="nlpE"
                     /locus_tag="RHW33_000913"
                     /gene_synonym="cutF"
     CDS             57092..57802
                     /gene="nlpE"
                     /locus_tag="RHW33_000913"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV1644454.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEAAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(58002..58826)
                     /locus_tag="RHW33_000914"
     CDS             complement(58002..58826)
                     /locus_tag="RHW33_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HDV1644455.1"
                     /translation="MDKPKAYYRLLLPCFLLLSACTVDISQPDSSATAVDVEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPSDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(58879..60597)
                     /gene="proS"
                     /locus_tag="RHW33_000915"
     CDS             complement(58879..60597)
                     /gene="proS"
                     /locus_tag="RHW33_000915"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV1644456.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVNAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60708..61415)
                     /gene="tsaA"
                     /locus_tag="RHW33_000916"
     CDS             complement(60708..61415)
                     /gene="tsaA"
                     /locus_tag="RHW33_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV1644457.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61412..61816)
                     /gene="rcsF"
                     /locus_tag="RHW33_000917"
     CDS             complement(61412..61816)
                     /gene="rcsF"
                     /locus_tag="RHW33_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV1644458.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61934..62749)
                     /gene="metQ"
                     /locus_tag="RHW33_000918"
     CDS             complement(61934..62749)
                     /gene="metQ"
                     /locus_tag="RHW33_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV1644459.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62789..63442)
                     /gene="metI"
                     /locus_tag="RHW33_000919"
     CDS             complement(62789..63442)
                     /gene="metI"
                     /locus_tag="RHW33_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV1644460.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63435..64466)
                     /gene="metN"
                     /locus_tag="RHW33_000920"
     CDS             complement(63435..64466)
                     /gene="metN"
                     /locus_tag="RHW33_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV1644461.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64654..65229
                     /gene="gmhB"
                     /locus_tag="RHW33_000921"
     CDS             64654..65229
                     /gene="gmhB"
                     /locus_tag="RHW33_000921"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV1644462.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15363 a        16253 c        18071 g        15665 t
ORIGIN      
        1 gcctaatcta tttgcatgaa gtgctatttc atgaatgaat taaaaaaaac taaaatgtaa
       61 attaagtata tttaatgtgt aaggggggat gtgaggagta agataagaaa ataatttttt
      121 cttggtggat gaaatagccg tatcattttg tattcctctc gttaagaaaa taaaataata
      181 cagctattac cagatggcag gttgtggtac tgctagtgaa gtgagtattt cacgtaatgt
      241 ttccccatca ggaaacacca actccggcgc gatttcaaac agcggccaca gcataaatcc
      301 acgatttttc atatcgtagt gcggtacggt cagtcgttca gtatttatca cttcattacc
      361 aaacagcatg atgtcgagat ccagcgtgcg cggtccccag cgttcagctt tacggacgcg
      421 accttgctgc aattcaatac gctgtgtgtg attgagtagc tcttcaggtg caagagaggt
      481 ttccagcgcc acggctgcgt ttaagtaatc gggttgatct tgcggcccca gcggtggggt
      541 gcggtaaaac gaagaaacgg taagaatgtg gctttcaggg atatcgccta atgctttcag
      601 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      661 tgtcatgcgg taccctcacg acgtggtgcg cgtttgcgtg gacgacgagt gcgacgacgc
      721 ggtgacggtt cttcatccag ctcgttgagc atcccttttt ggtctggtgg cgcggaaacc
      781 tggaactcac cccaccattt caccagacgc tgcagttcag cgttacgctc aacttcagct
      841 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgca
      901 cgtttaccct gacgtcggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtt
      961 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
     1021 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1081 agcagtgggt accagaacat ggcggcaaac aggaacgccg ggttcacgcg catatcgtta
     1141 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1201 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1261 cacagcagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1321 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1381 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1441 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1501 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1561 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1621 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1681 tggtgtccac ggaaggttgc aacttcgata atctccgggc caaacatcac atgagccaga
     1741 cggaaacggc gacctaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1801 gcgttagtgg ttacgtcaaa atctttcggc tttttgccga gtaacaggtc gcgcacgccg
     1861 ccgccaacca gccaggcttc gtatcccgct ttattgagcc tgtacattac cttcagggca
     1921 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac cgtcacctgt
     1981 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     2041 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2101 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2161 actcaggcac tgcggtaagc cgccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2221 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2281 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2341 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2401 ggtacagcga gatttgccgc actgtcggtt cgatcaaatc tgccccgcgg acaatttcgc
     2461 tcacgccctg gaagtgatca tcgaccacca ccgccaggtt ataggcaaac aaaccatcac
     2521 ggcgatgaat aataaaatct tcccgtgcca gtttttcgtc ggcgtgaatg ctgcctcgca
     2581 gcaggtcagt aaactgggtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2641 gtccatgatg caattcccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgtg
     2701 cacgcgtgca ggtgcagtaa taacttagtc cttgctcaag taaccaggcg agtgcttcgc
     2761 gataggcgtg gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc aggccgtaat
     2821 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2881 cttctatgcg taccagccag cgaccttgcc gggcgtgagc ctgcaaatag ctgccaagcg
     2941 cagcaatcag agaaccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     3001 agtgtgtgtc tgtcatctct tcgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3061 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3121 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3181 caacaccgca ggattcacag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3241 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3301 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3361 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3421 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3481 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3541 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3601 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3661 gaaaagcgta tttgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3721 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3781 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3841 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3901 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3961 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     4021 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttctgcgc
     4081 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4141 ggatagcctc tttcgtcaac ctgttagccc aaagcgtgtt gacgcaaata aatgcgccgc
     4201 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4261 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4321 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4381 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4441 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4501 gtaaagggtg aactccgtca ccgcatacga ccagttaaaa cctggcggcg ggattgtcac
     4561 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4621 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4681 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4741 cgtgagtgtg aaaccaggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4801 ctcggcgctc acgtcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4861 acgtcccgcc tcaagtggga agtgtgtggc gcgccagtgg ataatctgtt cgcggatttc
     4921 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4981 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     5041 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5101 gcaggtgtta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5161 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5221 gcgtaacgtc gcgcaacggc tggcggaact gcttaacgaa aagccaggcg ataccgttgg
     5281 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5341 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gactggtgat
     5401 ccttgatgaa tttcatgagc gcagcttgca ggcggatttg gcgttggcgc tgttactcga
     5461 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggataa
     5521 cgaccgcttg cagcaaatgc tgccagaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5581 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgtcgc
     5641 ggttgccacc gccgaaatgc tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5701 cgtcggagaa attcagcgtg tgcaggaaca actggcttcg cgcatcggca gtgatgtgtt
     5761 gctctgcccg ctgtatggcg cgttgtcgct gaacgatcag cgaaaagcga tcctcccggc
     5821 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5881 tgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgtt ttgatccgcg
     5941 cacggggctt acgcgactga ttactcaacg cgttagccag gcatccatga cgcagcgtgc
     6001 cgggcgcgcc gggcgtctgg agccgggtat ctgcctgcat ttaatcgcca aggaacaagc
     6061 agaacgcgcc gcggcgcaaa gtgaaccgga aatattacaa agcgatcttt ccggtttgct
     6121 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6181 gccaacggtg aatctactgg ccgcgaaacg cctgttacgg atgctggggg cgctggacgg
     6241 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6301 ggcaatgctg gttagcgcaa agagcgacga cgaagctgct accgcggcaa aaattgccgc
     6361 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggtgtggcgt tttcgcgcaa
     6421 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6481 tgaggcagac agttcgctta tcgcgccgct acttgccggg gtgtttgccg atcgcattgc
     6541 tcgccgccgt gggcaagatg gacgctatca actggcaaac ggcatggggg cgatgcttga
     6601 tgccgacgac gcgctaagtc gccacgaatg gttgatcgcg ccgttattat tgcagggcag
     6661 cgcctcgccg gatgcgcgga ttttactggc gttaccggtc gatattgatg aattagtaca
     6721 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6781 gctgaaagcc tggcgtcggc tgcaaatcgg tcagttgacg ttgaaagtgc agccgttggc
     6841 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaac ggcatccgtg ataaaggttt
     6901 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgg
     6961 aaagtggttg ccagaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     7021 ggaaacgtgg ctgctgccac atatggccgg tgtacattca ctacgcggcc tgaaatcact
     7081 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7141 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7201 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7261 gacgatcgcc caggggcgcg tgccgctggt gctggaattg ctttcacctg cccaaaggcc
     7321 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7381 agagatgaaa gggcgttatc ccaaacacgt ctggccggac gacccggcaa acacggcacc
     7441 gacgcgacgg acgaaaaagt attcgtaaaa cgggtatgag cagtcccctc gcccctatgg
     7501 ggagagggat agggtgaggg gtaatgtgcg cgctgatgcc ctcaccccgg ccctctccca
     7561 cagggagagg gagaaaagca aatttgagag atatcttctt ctgtcttgca acagaagaac
     7621 agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc
     7681 gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt
     7741 cgttacgaag atgacgacta cgacgattat gatgactatg aggaagaaga accgatgccg
     7801 cgcaaaggta agggcaaagg gcgtaagcct cgtggcaaac gcggctggct atggctgctg
     7861 ttaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta tctcgatcaa
     7921 aaaattcgta gtcgtattga tggcaaggtc tggcaattgc ccgcggcagt ttatggccga
     7981 atggtcaacc ttgagccaga catgaccatc agcaagaacg agatggtgaa gctgctggag
     8041 gcaacccagt atcgtcaggt gtcgaaaatg acccgccctg gcgaatttac cgtgcaggcc
     8101 aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga agggcaggtg
     8161 cgtgcgcgtc tgacctttga tggcgatcat ctggcgacga tcgtcaatat ggagaacaac
     8221 cgtcagtttg gcttcttccg tcttgatcca cgcctgatca ccatgatctc ttcgccaaac
     8281 ggtgagcaac gtttgtttgt gccgcgcagt ggtttcccgg atttgctggt ggatactttg
     8341 ctggcgacag aagaccgtca tttctacgag catgacggca tcagcctcta ctccatcgga
     8401 cgtgcggtgc tggcaaacct gactgccgga cgcacggtgc agggagcgag tacactgacg
     8461 caacagctgg tgaaaaacct gttcctctcc agcgaacgtt cttactggcg taaagcgaat
     8521 gaagcctaca tggcgctgat catggacgcg cgttacagca aagaccgtat tcttgagctg
     8581 tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg cttcccgctg
     8641 gcgagcttgt attactttgg tcgcccggta gaagaattga gcctcgatca gcaggcgctg
     8701 ttggttggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg
     8761 ctggagcgac gtaatctggt actgcgtctg ctgcaacagc aacagattat tgatcaagaa
     8821 ctctatgaca tgttgagtgc gcgtccgctg ggggttcagc cgcgcggtgg ggtgatctct
     8881 cctcagccag cctttatgca actggtgcgt caggagcttc aggctaaatt aggcgataag
     8941 gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc ccaggacgcg
     9001 gcagaaaaag ccgccgtgga aggcattccg gcacttaaga aacagcgtaa gctgagcgat
     9061 ctggaaaccg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc gatggtcgga
     9121 ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcggcg ttcgattggt
     9181 tcccttgcaa aaccagcgac ttatctgacg gccttaagcc agccgaaaat ctatcgtctg
     9241 aatacgtgga ttgcggatgc gccaatcgcg ctgcgtcagc cgaatggcca ggtctggtca
     9301 ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt ggatgcgttg
     9361 acccgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct gcctgcggtt
     9421 acggagacct ggatcaaact gggtgtaccg aaagatcagt tgcatccggt tccggcaatg
     9481 ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca gactatcgcc
     9541 agcggcggta accgtgcacc gctttctgcg ctgcgttcgg taattgcgga agatggcaaa
     9601 gtgctgtatc agagcttccc gcaggcggaa cgcgctgttc cggcacaggc ggcgtatctg
     9661 acactatgga ccatgcagca ggtggtacaa cgcggtactg ggcgtcagct tggggcgaaa
     9721 tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt agatacctgg
     9781 tttgcgggca ttgacggcag cacggtgacc atcacctggg tcggccgtga taacaaccag
     9841 ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta tctggctaac
     9901 cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat gggcgtggac
     9961 tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg gaccagcgat
    10021 ccgcaatcgc tgtgccagca gagtgagatg cagcagcagc cgtcaggcaa cccgtttgat
    10081 cagtcttcac agccgcagca acagccgcaa cagcaacctg ctcagcaaga gcagaaagat
    10141 agcgacggtg tagccggttg gattaaggat atgtttggta gtaattaaca tctaagcgtg
    10201 aaataccgga tggcgagttg ccatccggta aaataacatc ccacccaaag tattaaccct
    10261 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10321 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10381 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10441 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10501 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10561 gaaagcgcat gggggcctgc tgcaactatt gcggcgcgac agtcagctac cggcactaaa
    10621 accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc cgaagagatg
    10681 gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg tgtctctgtt
    10741 ggtacgcgtg gcgcatccaa cacctatgac cacctgatca ttcgcggttt tgcggcagaa
    10801 ggccaaagcc agaataacta tctgaatggc ctgaagttgc agggcaactt ctataacgat
    10861 gcggtcattg atccatatat gctggaacgc gctgaaatta tgcgtggccc ggtttccgtg
    10921 ctttacggta aaagcagtcc tggcggcctg ttgaatatgg tcagcaagcg tccgaccacc
    10981 gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca gactggtttt
    11041 gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac cggtcttgcg
    11101 cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat tgcaccggcg
    11161 ttcacctggc gtccggatga taaaaccaat ttcaccttcc tctcttactt ccagaacgag
    11221 ccggaaacgg gttattacgg ctggttgccg aaagagggaa ccgttgagcc gctgccgaac
    11281 ggtaagcgtc tgccgacaga ctttaacgaa ggggcgaaga acaacaccta ttctcgtaat
    11341 gagaagatgg tgggctacag cttcgatcac gaatttaacg acacctttac tgtgcgtcag
    11401 aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta cggcgtctgc
    11461 tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc ggataaaggc
    11521 cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt ctccgttgat
    11581 acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct gaccggtgtc
    11641 gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga ctccgtaccg
    11701 ctgctcgatc tgtacaatcc ggtgaatacc gatttcgact tcaatgccaa agatccggca
    11761 aactccggcc cttaccgcat tctgaataag cagaaacaaa cgggcgttta tgttcaggat
    11821 caggcgcagt gggataaagt gctggtcacc ctgggcggtc gttacgactg ggcagatcaa
    11881 gaatctctta accgcgttgc cgcgacgacc gataaacgcg atgacaaaca gtttacctgg
    11941 cgtggtggtg ttaactacct gtttgataat ggcgtaacac cttacttcag ctatagcgaa
    12001 tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc gtctaaaggt
    12061 aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt agttactggt
    12121 gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg ttccttcttc
    12181 tcggttgaag gtggtgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa agcggcgctg
    12241 tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata caccaccgat
    12301 actacctata aaggcaatac gccggcacag gtgccaaaac acatggcttc gctgtgggct
    12361 gactatacct tctttgacgg tccgctttca ggtctgacgc tgggcaccgg tggtcgttat
    12421 actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta tacggtcgtg
    12481 gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa cgtggcgctg
    12541 catgtcaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac ttatggctgc
    12601 ttctggggcg cagaacgtca ggtcgttgca accgcaacct tccgtttcta atttctcttt
    12661 tggggcacgg atttccgtgc ccgtttcaca agttggctgt tatgcaggaa tacacgaatc
    12721 attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt
    12781 tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt ggtcacaacg
    12841 gttctggtaa atccactctg ctcaaaatgc ttggccgtca tcagccgccg tcggaagggg
    12901 agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt gcccgcaaag
    12961 tggcttattt accgcagcag cttcctccgg cagaagggat gaccgtgcgt gaactggtgg
    13021 cgattggtcg ttacccgtgg catggcgcac tagggcgctt tggtgcggca gatcgcgaaa
    13081 aagttgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg ctggtcgata
    13141 gcctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg caggatagcc
    13201 gttgtctgtt gctcgacgag ccaacctcgg cgctggatat cgcccaccag gttgatgtgc
    13261 tggcgctggt gcatcgttta agtcaggaac gtggtctgac ggtcattgcc gtgctgcacg
    13321 atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcggc ggtgaaatga
    13381 ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg atttatggca
    13441 tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt gtttattgat
    13501 gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc tttctccgtt
    13561 gttatggcag atgaataccg cccacgcggc ggctattgat cccaatcgta ttgtggcgct
    13621 ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg gcgtggcgga
    13681 taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag tgattgacgt
    13741 tggtttgcgt acagaaccta acctcgaact gctgaccgaa atgaaaccgt cgtttatggt
    13801 ctggtcggca ggatatggcc cttcaccaga aatgctggct cgtattgcgc cggggcgtgg
    13861 atttaacttc agcgacggca aacagccgct ggcgatggcg cgtaaatcgc tgacggaaat
    13921 ggcggattta cttaacctgc aaagcgcagc agaaacgcat ttagcgcaat atgaagactt
    13981 tatccgcagc atgaaacccc gctttgtgaa gcgtggtgcg cgtccgttat tgctgacgac
    14041 gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc aggaaattct
    14101 cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg gcagtaccgc
    14161 cgtcagcatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt ttgatcacga
    14221 caacagcaaa gacatggatg cgctaatggc aacgccgctg tggcaggcca tgccgtttgt
    14281 ccgcgccgga cgctttcagc gcgtacccgc agtctggttt tatggtgcaa cgctctcggc
    14341 aatgcacttt gtgcgcgttc tggataacgc cattggaggt aaagcgtgag taaacgtatt
    14401 gcgcttttcc cggcattatt gctggcgctg ttagtgattg tcgctacggc gctcacctgg
    14461 atgaacttct cgcaggcgct gccgcgtagc cagtgggcgc aggctgcctg gtcgccggat
    14521 attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct ggcgatttcg
    14581 ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaac
    14641 ccgctggcgg aaccgacgac gttgggcgtt gctacaggcg cgcaactggg gattaccgtc
    14701 actacgctct gggcgatccc tggtgcgatg gcgagccagt ttgctgcgct ggcaggggct
    14761 tgtgttgttg gcttaattgt ctttggcgtt gcgtggggga aacggctgtc gccggtaacg
    14821 ctgattctcg cggggctggt agtgagcctt tattgtggag caatcaatca attactggtt
    14881 atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac gctgacgcaa
    14941 accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt gatgctgacg
    15001 ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc gcgcaatctc
    15061 gggctggcct tgtcgctggc gcgtctggcg gcgctgtcgc tggcgattgt catcagtgcg
    15121 ctgctggtga atgcggtagg gattatcggc tttatcgggt tgttcgcgcc gctgctggca
    15181 aaaatgctgg gggcgcggcg tctgctgccg cgactgatgc tggcgtcgtt gattggtgcg
    15241 ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg
    15301 tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggtt gctgccgcgt
    15361 ttacgcagca ttagcgcgcc ggatatgaag gtcaacgatc gtgtcgcggc tgaacgccaa
    15421 catgtgctgg cgtttgccct cgcgggcggc gtgctgctgt tgatggctgt ggtggtggcg
    15481 ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt gctcgatgat
    15541 ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt catgctggcg
    15601 gtggcgggct gtattattca gcggctgacc ggaaacccga tggcaagccc ggaagtgctg
    15661 gggattagct ctggcgcggc gtttggcgtg gtgttaatgc tgtttctggt gccgggtaat
    15721 gcctttggct ggctgttacc cgcgggcagt atcggtgccg cggtgacgct gttgattatt
    15781 atgatcgccg ccgggcgcgg tggattttcc ccacaccgca tgttactggc ggggatggcg
    15841 ttaagtaccg cattcaccat gcttttaatg atgttgcagg cgagtggtga cccgcgaatg
    15901 gcgcaagtgc tgacctggat ctctggttcg acctacaacg ccaccgatgc gcaggtctgg
    15961 cgcaccggaa ttgtgatggt gattttgctg gcgattaccc cgctgtgccg ccgctggctg
    16021 accattttac cgctgggtgg tgataccgcg cgggccgtgg gggtggcgtt aacgccgacg
    16081 cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat gaccattgga
    16141 ccgttgagtt ttgttggttt aatggcaccg catatcgcgc ggatgatggg ctttcgacgg
    16201 acgatgccgc acatcgtgat ttcggcgctg gtgggtggtt tactgctggt gttcgctgac
    16261 tggtgtgggc ggatggtgct gtttccgttc cagatcccgg cggggctgct gtcgaccttt
    16321 atcggcgcgc catattttat ctatttgttg agaaagcaga gccgttaata aaatgcctcg
    16381 cccggtgggc gaggcaaaat catcacaact tcgcaaacac ccgacgtgca gcatcgatgg
    16441 tgttattgat atcttccatg ctgtgcgcca cggacataaa gcccgcttca aacgctgacg
    16501 gtgccaggta aacaccttcg tccagcatca tatggaagaa acgcttaaag cgttccacgt
    16561 cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag aaaataccga
    16621 acatgccgcc aacgtggtta acgaccagcg gaattccggc ttcttctgcc gcttccagca
    16681 gaccttctgc cagacgtgat gtcagctcat ccaacgtttc gtgaacgccc ggctgcgcga
    16741 cttcattcag acaggcgaaa cccgctgcca tcgcaattgg gttaccggaa agcgtacccg
    16801 cctgatagac cggacccgtc ggggccagcg catccattac atcacgacga ccaccgaatg
    16861 cgcctaccgg cattccaccg ccgatgattt tgcccaggca ggtgagatcc ggttccacac
    16921 cgtaataatc ctgtgcgcca gccagtgcca cgcggaagcc ggtcattact tcatcgatga
    16981 tcagcaatgc gccaaattcg tcacacagcg cacgcagacc tggcaggaac tctggcagcg
    17041 gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca atctcttgcg
    17101 ggtattgctc aaacgcggcg cgtacagaag ccagatcgtt ataagtacag gttaaggtat
    17161 gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg agtgcgccag
    17221 aaccggcttt taccagcagg cagtcagcgt gaccgtggta acaaccttca aatttaataa
    17281 ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatggtc gcctcggtgc
    17341 cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc agttgcgcca
    17401 ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca
    17461 cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag gaaccgacat
    17521 aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct ttttcgataa
    17581 acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga
    17641 tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt tcctgattcg
    17701 tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata ctcgtcaaac
    17761 atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt ttgtgcattt
    17821 gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta
    17881 tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt agaaacaccg
    17941 caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg cgataaaacc
    18001 ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttgtcgggct ggctgcggtt
    18061 gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct ggtacatact
    18121 gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt gctggcgatg
    18181 ttcggctact ttttggtgcg caaatacgcg cccgaagcag gggggtcggg gatcccggaa
    18241 attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt gccggtgaaa
    18301 ttctttggcg ggctggggac actcggcgga ggcatggtgt tggggcgcga agggccaacc
    18361 gtgcagatcg gcggtaacat tggccgtatg gtgcttgata ttttccgcct gaaaggtgac
    18421 gaagcgcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggcggc ggcctttaac
    18481 gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt tcgctatacg
    18541 ttaatttcga taaaagcggt atttattggc gtcattatgt cgaccattat gtaccggatt
    18601 tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg
    18661 ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat ttttaataaa
    18721 tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg
    18781 gtgctgatgg gcggtgcgat tggcggtctg tgtggattgc tggggtttgt ggcaccagca
    18841 acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt cagcatggga
    18901 atgctggtgt ttatcttcgt cgcgcgggtc attaccacct tactctgctt ctcttccggc
    18961 gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg aaccgctttc
    19021 ggaatggttg ccgttgagct gtttccgcaa tatcaccttg aggcggggac gtttgctatt
    19081 gccggaatgg gggcgttact ggcggcatct attcgcgcgc cgttaacggg gatcattctg
    19141 gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac cggtcttggc
    19201 gcgacactat tagcgcaatt taccggcggg aaaccgctat actcggcgat tcttgcgcgc
    19261 acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc agccagcgag
    19321 aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg ttagaatttg
    19381 cccaattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact gccgctggag
    19441 tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga taacccgaat
    19501 ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta tggtttcacc
    19561 tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt tggcttggtg
    19621 gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactatac cgaaggactg
    19681 gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct
    19741 tcctttagta tctaatctgt tgttcatgtg ccggataaac tatccggcac atcttctctc
    19801 ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa cgaatagccg
    19861 ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc agtggtacgg
    19921 aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca gttgcgtaga
    19981 tttctgtacg taaaatcatg gggatttcgc gggccagaac atcacgaatg atcccgccgc
    20041 caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc gcattaaagg
    20101 ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc accggcaaca
    20161 tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg ctggttacca
    20221 ttgcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga tccagcgcca
    20281 tgtcgcgaat tgtcccgccg cctacagcgg taaccacgcc aagtaccaga acaccaaaag
    20341 ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc gctgtgccga
    20401 ctatatccag ccaatagacg agcattgttc aatccccact gagcacctaa aaaggtcagg
    20461 cgctaatcta cctgtgaaag cgcattacag agctgttgtg cagcgaggat aatacgtggg
    20521 cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag ctgttcaccc
    20581 cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat cgcctgtggc
    20641 gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct gtctttaaag
    20701 atgttttctc cgccacaaac ttcgagtacc tggttttgaa tcgactcttt tccagtggta
    20761 aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc agcatattgc
    20821 gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc cttatccggt
    20881 tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca
    20941 tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg ctcggcatta
    21001 cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc cagattcatc
    21061 ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg
    21121 ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc gggagaaagc
    21181 gtgatgacgc gcggtgccgc gttgagccac agtggcgcga gaaaagacag ggcgaccagc
    21241 gccctgaaca gtgacttagc catgtgccag tttctgcacc agtgactcaa ccatcaggct
    21301 ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact gttgatcggc
    21361 cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc agacatgggc
    21421 gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc ggattttcgc
    21481 cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt
    21541 aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt cagctttaaa
    21601 gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga catccgcgtc
    21661 gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg gtgccaggcc
    21721 accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca gcaaagtggc
    21781 acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct cggttccatt
    21841 cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt tttcgatttt
    21901 gtcacgcagc agcgtgactt cttcttccat tgcaccaatg atgccgattt tcatagattt
    21961 actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg aagcgtattt
    22021 ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac tggcatcgtc
    22081 gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc ttcgagagcg
    22141 atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt
    22201 ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc
    22261 aggtggggcg ctacatcgcc aaagaaattt taagccgtct gaaagagctt aaattgctgg
    22321 aagcatacgg cctggatgaa ctgaccggac cttttgaaag cattgttgag atgtcatgcc
    22381 tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg ataaatgact
    22441 ggtttcgcca gcgtttgcac ccggaagatg ccgaaagcca gcctctgact gacgatcgct
    22501 gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag ctgcggcgca
    22561 agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt ctggtgcata
    22621 cattgatgcg gatgaatctc acctgggcac aggttggcgg tattttaaaa tatacccgtc
    22681 cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa aagccgggtt
    22741 attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat ttggcgcttt
    22801 acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc tattgtgtgg
    22861 cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt tatcatcatt
    22921 tgcacgaggc gtggggtcag catgagaaag gttcgctctt ttcgctagtg gttgaaaatg
    22981 cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag ttttttatgt
    23041 atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga tttattgata
    23101 atctgcctac gattttcgcc ggaacgttta atcatgcatt attggaagat gccagcgaat
    23161 gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt agccatccag
    23221 atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta gagatttatc
    23281 gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa gaacgggtga
    23341 aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat cggctggcct
    23401 atgtcgaagc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta tgggaatatt
    23461 attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc tatgcgtggg
    23521 atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa gacgaacaat
    23581 aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa cgaatctgaa
    23641 gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa aaaaccacat
    23701 tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc tctgcaacgg
    23761 cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca ccgatgctcg
    23821 aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc gttaatacgc
    23881 cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc tgccaggaag
    23941 gttctccgtt ccagagctct ccgttctgcc agggtggcca gggcggtaat ggtggcggcc
    24001 agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat aaaggctatg
    24061 tcgttaccaa caaccacgtt gttgataacg cgacggtcat taaagttcaa ctgagcgatg
    24121 gccgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc gcgctgatcc
    24181 aaatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat gcactgcgcg
    24241 tgggtgatta caccgtagcg attggtaacc cgtttggttt gggcgagacg gtaacttccg
    24301 ggattgtctc tgcgctgggg cgtagcggcc tgaatgccga aaactacgaa aacttcatcc
    24361 agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac ctgaacggcg
    24421 aactgatcgg tatcaacacc gcgatcctag caccggacgg cggtaacatc ggtatcggtt
    24481 ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa tacggccagg
    24541 tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ctccgaactg gcgaaagcga
    24601 tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat tcctccgctg
    24661 caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag ccgatcagca
    24721 gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa ctgaccctgg
    24781 gcttgctgcg cgacggtaag caggtcaacg tgaatctgga actgcagcag agcagccaga
    24841 atcaggttga ttccagctcc atcttcaacg gcattgaagg tgctgagatg agcaacaaag
    24901 gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct gcgcagatcg
    24961 gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa aacatcgctg
    25021 aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt cagcgcggcg
    25081 acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga aaacgggaag
    25141 gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat catcctttag
    25201 gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca taacggagga
    25261 cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc acgtaccatg
    25321 cgcatcatcg ataccaatat caacgtaatg gatgcccgtg ggcgaattat cggcagcggc
    25381 gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc acagggacga
    25441 gtcgtcgata tcgatgacgc ggtggcacgt catctgcacg gtgtgcggca agggattaat
    25501 ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag
    25561 aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct ggaacagtcg
    25621 cggttgatgc acttgttggc gcaggatagc cgtttgcggg aggaactggt gatgaacctg
    25681 attcaggcag aggagaatac tcccgcactt actgaatggg cgcaacggct ggggatcgat
    25741 ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct tggtgtggac
    25801 agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga gcgtaataat
    25861 ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt gaactctttt
    25921 gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac ccgcatgaaa
    25981 gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg tcctggcagt
    26041 attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca gcggatgcca
    26101 gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga cagtttgcgt
    26161 ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc gatggacaat
    26221 aacggcttgc tgcgacgaac gctggcggcg tggtttcgtc acaatgtgca accgctggca
    26281 acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa tcgtatatcg
    26341 gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta tgtggcgtta
    26401 caactggatg aagagcggta ggttatgcgt taaggttgag gcggcgctgg ctcattcccc
    26461 tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat tatttacgcg
    26521 ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact gactccaggt
    26581 gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca cgctggcaga
    26641 tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg acttctttat
    26701 aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat ttaaacttaa
    26761 cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga atatcgttgt
    26821 tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata cccagacttt
    26881 tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct tattttagca
    26941 tttggcctgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt ccagacttct
    27001 atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc atgggatgga
    27061 ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga tgaaaaagta
    27121 tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgaaccaga aagcaatcgg
    27181 gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg
    27241 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27301 tatttgccat ctttcgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga
    27361 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    27421 cccatggaaa tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgcgcg
    27481 ccgatgaagc aattatcttc aatgatggtt gggttagcct gcagcggttc cagcacaccg
    27541 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    27601 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac
    27661 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc aggtggtaca
    27721 acgcggaagc cttctttctg gaaacgtact tcgtcgtagt cggcgaattt catcggcact
    27781 ttatcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    27841 agcagtaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    27901 acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    27961 gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    28021 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28081 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca
    28141 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28201 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg cgccagcagc
    28261 ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg ggttggcaaa
    28321 aacgtcactt cggtttcaac agtaaaatgg cgcaatttgg cgggttggcg acggggctgc
    28381 ggtggctgcc agctactttg tgtcagtact tgctccagac caaaccgaat aacctcatgg
    28441 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    28501 ccgtcgcgag tggtgaaaat ttgtgcgtcg tggacactta aattgcggcg gtctaattcg
    28561 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    28621 ccacgcgtag cctgcggact aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa
    28681 tggcgcgcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    28741 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag cagtgccagc
    28801 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    28861 cgtagctgtt tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    28921 cacagcgttt cgttggtggc gcaaatgtca gccaccgtca ggcataccag ataacgaagt
    28981 cggttttctg tttgcacttc ttcggcaaac tgcttgatga cttccgggtc ctgaatatcg
    29041 cggcgttggg cggtcaccga catcaacagg tgctggcgaa ccagccacgc caccagttgc
    29101 gtttcgcgcg agttaagccc gtggagttcg gcaaaatgca ctacatcctg agcaccgaga
    29161 atggagtgat cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29221 agctcaggtg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29281 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29341 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gtgaccattg cggcatatac
    29401 gcgccgagta cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga
    29461 cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg ttgttgcaga
    29521 tggcgccggg catggcgtaa ctggcgcaac gtggtggagt aaatgccggt gatcgcactg
    29581 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    29641 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt
    29701 ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    29761 agttcactga cgcggcgtgt cacgcggaag taatccttca tcatccgctc gaccggttcg
    29821 ttaccttcgc cactgtaatt cagacgctgg gcaacgctaa gctggcgatc gaataacagg
    29881 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca caatatatgc
    29941 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca
    30001 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc
    30061 aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc
    30121 tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat
    30181 ccttcgctga aaatatgttt ttgcagttca agaaatagcg caacatcgcc aattaataag
    30241 cgggattcga tcaaattggt ggcgacggtt aaatccgata acccttccag tatgcactct
    30301 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30361 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    30421 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg
    30481 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac
    30541 tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    30601 caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc
    30661 cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt
    30721 tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg
    30781 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    30841 gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    30901 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    30961 tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt
    31021 ggtttcacgg gagtcatagt gcagcacctg cggttcttcg tggaagccgc gaccaatacc
    31081 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt tctgaatcgc
    31141 cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca ggtacaggct
    31201 ttcttgcgtg atacggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat
    31261 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31321 gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt cattaataga
    31381 aatgcaaacg gatttcggat aaccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    31441 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    31501 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    31561 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata
    31621 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc ctggcaaatt gagaatcatt
    31681 ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg acgtctgatg
    31741 ctgtacacag cgccaacaat tattggtgtc cacggcgtat ttgtggtata aagcgcgccg
    31801 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    31861 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    31921 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    31981 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    32041 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32101 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat
    32161 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32221 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32281 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32341 caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    32401 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    32461 ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt
    32521 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    32581 ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca
    32641 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcacgctcgt
    32701 acgagccctt atagaccagg tagtacacgt ttggttaggg ggcctgcata tggccccctt
    32761 tttcactttt atatctgtgc ggtttaatgc cgggcagatc acatctccga ggattttaga
    32821 atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg agcgtactgg cgcaggcatg
    32881 atggattgca aaaaagcact gactgaagct aacggcgaca tcgagctggc aatcgaaaac
    32941 atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag gcaacgttgc tgctgacggc
    33001 gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc tggaagttaa ctgccagact
    33061 gacttcgttg caaaagacgc tggtttccag gcgttcgcag acaaagttct ggacgcagct
    33121 gttgctggca aaatcactga cgttgaagtt ctgaaagcac agttcgaaga agaacgtgtt
    33181 gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc gcgttgctgc gctggaaggc
    33241 gacgttctgg gttcttatca gcacggtgcg cgtatcggcg ttctggttgc tgctaaaggc
    33301 gctgacgaag agctggttaa acacatcgct atgcacgttg ctgcaagcaa gccagaattc
    33361 atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag aataccaggt acagctggat
    33421 atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga aaatggttga aggccgcatg
    33481 aagaaattca ccggcgaagt ttctctgacc ggtcagccgt tcgttatgga accaagcaaa
    33541 actgttggtc agctgctgaa agagcataac gctgaagtga ctggcttcat ccgtttcgaa
    33601 gtgggtgaag gcatcgagaa agttgagact gactttgcag cagaagttgc tgcgatgtcc
    33661 aagcagtctt aattatcgaa aaggagccgc ctgagggcgg cttctttttg tgcccatctt
    33721 gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg acgtacaatg tcgctagtat
    33781 taattcattt caatcgttga cagtctcagg aaagaaacat ggctaccaat gcaaaacccg
    33841 tctataaacg cattctgctt aagttgagtg gcgaagctct gcagggcact gaaggcttcg
    33901 gtattgatgc aagcatactg gatcgcatgg ctcaggaaat caaagaactg gttgaactgg
    33961 gtattcaggt tggtgtggtg attggtgggg gtaacctgtt ccgtggcgct ggtctggcga
    34021 aagcgggtat gaaccgcgtt gtgggcgacc acatggggat gctggcgacc gtaatgaacg
    34081 gcctggcaat gcgtgatgca ctgcaccgcg cctatgtgaa cgctcgtctg atgtccgcta
    34141 ttccattgaa tggcgtgtgc gacagctaca gctgggcaga agctatcagc ctgttgcgca
    34201 acaaccgtgt ggtgatcctc tccgccggta caggtaaccc gttctttacc accgactcag
    34261 cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt gctgaaagca accaaagttg
    34321 acggcgtgtt taccgctgat ccggcgaaag atccaaccgc aaccatgtac gagcaactga
    34381 cttacagcga agtgctggaa aaagagctga aagtcatgga cctggcggcc ttcacgctgg
    34441 ctcgtgacca taaattaccg attcgtgttt tcaacatgaa caaaccgggt gcgctgcgcc
    34501 gtgtggtaat gggtgaaaaa gaagggactt taatcacgga ataattcccg tgatggataa
    34561 ataagggtaa gattccgcgt aagtatcgcg ggggatctta cccgtaatat gtttaatcag
    34621 ggctatactt agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc
    34681 aaggattcgt aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg
    34741 cgtagaagcg ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct
    34801 gctggatggc attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag
    34861 cgtaacggta gaagattccc gtactctgaa aatcaacgtg tttgatcgtt caatgtctcc
    34921 ggccgttgaa aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag
    34981 cgacatccgt gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat
    35041 cgttcgtggt gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa
    35101 cgacaaagtg aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc
    35161 tcaggacgat gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc
    35221 agacaaagaa gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt
    35281 agatccttgc ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt
    35341 tcatgaagca actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg
    35401 tggtgcgcca taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca
    35461 ctcgcatggt agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag
    35521 cgagtgcgaa acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa
    35581 gtgggcaaca agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag
    35641 ccattgttgg cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca
    35701 ttttgctggc caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa
    35761 agcagagcaa agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt
    35821 taccgcaacc tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt
    35881 ccattttact taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa
    35941 caatgacgcc cgatcaagcc tgccgtcatc cgaactggtc gatgggacgt aaaatttctg
    36001 tcgattcggc taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta
    36061 acgccagcgc cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg
    36121 tgcgctatca ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa
    36181 ttgcccacac catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt
    36241 gcaaactaag tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac
    36301 tggcgatgga ggcgttcgaa caaggccagg cagcgacgac agcattgaat gccgcaaacg
    36361 aaatcaccgt tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga
    36421 atttatccgt actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat
    36481 ctgttgatgc gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag
    36541 gataatccgg ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct
    36601 gcgccatctg atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg
    36661 cttttttttg tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa
    36721 acgcgtgatg ttgtctgcca ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg
    36781 tcatgttgcg atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg
    36841 tgcctttggg cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa
    36901 caacggtatt gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc
    36961 gcaggaagtc agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag
    37021 tctgcaccga cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg
    37081 tttgcaagaa cgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct
    37141 gaatattgcg gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc
    37201 tgaaaaggtg cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca
    37261 gcatgtctgt atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca
    37321 tcgcattagt aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt
    37381 tctctggccc gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg
    37441 agagcgtcgt ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt
    37501 gctgaagtat cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt
    37561 tctgttgccg ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg
    37621 ggaatgggga cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt
    37681 atgcgggtta ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca
    37741 tcaaccgctg gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt
    37801 gctggtgctg ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct
    37861 tatttttggc gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg
    37921 gcactatgac gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt
    37981 atggggcgct gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc
    38041 accgaaggtt tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc
    38101 ggtaatctca tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact
    38161 catttgctct attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt
    38221 taagcgtgaa gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt
    38281 agatcgtatt gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt
    38341 attcaggacg ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc
    38401 atcgttgcac tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg
    38461 cgttgtggtg ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga
    38521 actgataagc tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa
    38581 atgctggatg agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat
    38641 aaatctgtcg gccaacgagc tgcgattatt gccgcaggtc cggttgcaaa cttcattttt
    38701 gctatctttg cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt
    38761 ggcgaaatag cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta
    38821 aaagccgtag atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat
    38881 aaaattggcg atgaaagcac caccattacg gtagcgccat ttggcagcga ccaacggcgg
    38941 gatgtaaagc ttgatttacg tcactgggcg tttgagcccg ataaagaaga tccggtatct
    39001 tcgttgggga ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcagcca
    39061 aactcggcgg caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt
    39121 cagcccttaa cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc
    39181 ttagcgttag aaatcgaaag gcaggggagt cccttgtctt tgacattaat cccggagagt
    39241 aaaccgggta atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg
    39301 ccagatgagt ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg
    39361 gacaaaacgt ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt
    39421 gatgtgaaac tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca
    39481 gcggaactcg gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg
    39541 ataattaacc tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc
    39601 gaaaagatca agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc
    39661 tcgattctgc tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga
    39721 gagttagtta ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc
    39781 tgctgtttag cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt
    39841 tcgaaggcct tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag
    39901 gcgacacggt taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca
    39961 actttgagga tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac
    40021 gtccgaccat tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga
    40081 agcaaaacct cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg
    40141 ccgatatcga gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg
    40201 taaaagctgt cgtgaccccg ctgccgcgca accgtgttga cttaaaactg gtgttccagg
    40261 aaggtgtgtc agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg
    40321 acgaactgat ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg
    40381 atcgtaaata ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agctactatc
    40441 tggatcgcgg ttatgcccgt ttcaacatcg attctaccca ggtcagtctg acgccagata
    40501 aaaaaggtat ttacgtcacg gtgaacatta ccgaaggcga tcagtacaag ctttctggcg
    40561 ttgaagtgag cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg
    40621 agccgggcga gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc
    40681 ttctcggtcg ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg
    40741 ccgacaaaac cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta
    40801 agatccgttt tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc
    40861 agatggaagg tgcatggctg ggaagcgatc tggtcgatca gggtaaggag cgtctgaatc
    40921 gtctgggctt ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc
    40981 aggttgatgt agtctacaag gtgaaagaac gtaacactgg tagcttcaac ttcgggattg
    41041 gttacggtac tgaaagtggc gtgagcttcc aggctggcgt gcaacaggat aactggttag
    41101 gtacaggtta tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac
    41161 tgtcggtaac caatccgtac ttcaccgtag atggcgtaag cctcggtggt cgtctcttct
    41221 ataatgactt ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta
    41281 cagacgtgac cttgggcttc ccgattaacg aatataactc gctgcgtgca ggtctgggtt
    41341 atgtacataa ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact
    41401 ctatgggtga acatccgagc acctctgatc aggataacag cttcaaaacg gacgacttca
    41461 cgttcaacta tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt
    41521 cacgtgtcaa cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag
    41581 tgacgttaga cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg
    41641 ggcgtacccg ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga
    41701 acttctatgc cggtggttcc agcaccgtgc gtggcttcca gtccaatacc attggtccga
    41761 aagcagttta cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg
    41821 cgactcagga cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca
    41881 tggcggttgc cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta
    41941 actcggttcg tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt
    42001 ccagccaata ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg
    42061 gtatcgcatt acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt
    42121 tcaaaaagta cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt
    42181 aagtgttctc cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata
    42241 agatcgccgg gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt
    42301 attgtgaaaa agtggttatt agctgcaggt ctcggtttag cactggcaac ttctgctcag
    42361 gcggctgaca aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa
    42421 accggtgttt ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt
    42481 atggaaaccg atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat
    42541 cgcactaagc tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag
    42601 gcttttgagc aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt
    42661 atccagactg ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca
    42721 aacgccgttg cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag
    42781 gttaaataag taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa
    42841 ctacacggtg atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca
    42901 ggtcacatta cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg
    42961 tccgcggttg tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg
    43021 aagaatccct acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc
    43081 gcgcagaaca ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta
    43141 tcgattggcg ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc
    43201 ggtgccggtt gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg
    43261 aacgtaacca tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca
    43321 gtggtaggcg cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca
    43381 cagattggtc gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat
    43441 cgcggcgcgc tggatgacac tattattggc aatggcgtga tcattgataa ccagtgccag
    43501 attgcacata acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg
    43561 ggcagcctga aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat
    43621 atggaaatat gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact
    43681 gaaccaggcg tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc
    43741 gctgcactgg tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag
    43801 gttaatcaac aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt
    43861 cttacgattg cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta
    43921 tcttgactac taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc
    43981 gtttcccttt cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg
    44041 cagtaaaaaa tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga
    44101 ttttcccggg tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta
    44161 aaagcgtagg aaaactggaa ccgggtgagc tgtactattt cgctggtatt gacgaagcgc
    44221 gcttcaagcg cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa
    44281 cgcgccgcgg cctgacccgt tttaaagggg ttgctctggt cgacggtaaa gtagtttgcg
    44341 aagcaacgat gatgtgtgct cgtagccggg aggcctgata cgtgattgat aaatccgcct
    44401 ttgtgcatcc aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac gcacacattg
    44461 gtcctttttg tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc
    44521 acgttgtcgt gaatggccat actaaaattg gccgcgataa tgagatttat cagttcgcct
    44581 ccatcggtga agttaaccag gatctgaaat atgctggcga accgacccgt gtggaaatcg
    44641 gcgatcgtaa ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg
    44701 gattgacgaa ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt
    44761 gtacggtagg taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg
    44821 tagacgacct cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg
    44881 cgcatgtgat ggttggcggt tgctccggtg tggcgcagga cgtccctcct tatgtcattg
    44941 cgcagggtaa ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat
    45001 tcagccgcga ggcgattacc gctatccgca atgcgtataa gctgatttat cgcagcggta
    45061 aaacgctcga tgaagtgaaa ccggaaattg ctgaactggc ggaaacgtat ccggaagtga
    45121 aagcttttac cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactgaaca
    45181 gcgtccatta acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg
    45241 tttaatccgc gctctgaaag aacgtgtgcc caacgcccgc tttgttggcg ttgccgggcc
    45301 acgaatgcag gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg
    45361 cattgttgaa gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa
    45421 gcgttttggt gaactaaagc cagatgtttt tgttggcatt gatgcgcctg acttcaatat
    45481 taccctcgaa ggtaacctta aaaagcaggg tatcaaaacc attcattatg tcagtccgtc
    45541 cgtctgggcg tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct
    45601 cgcatttctg cctttcgaaa aagcgtttta tgacaagtat aacgtgccgt gccgttttat
    45661 cggtcacacg atggctgatg ccatgccgtt agatccagat aaaaacgccg cccgtgatgt
    45721 gctgggtatc cctcacgatg cccactgtct ggcattgttg ccgggcagcc gtggcgcgga
    45781 agtcgaaatg cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc
    45841 ggatctcgag atcgtggtgc cgctggtgaa tgccaaacgc cgtgagcagt ttgagcgtat
    45901 caaagcggaa gttgcgccag acctgtcggt tcatttgctg gatggaatgg gccgtgaggc
    45961 gatggtcgcc agtgatgcgg cgctactggc gtcgggtacg gcagccctgg agtgtatgct
    46021 ggcgaaatgc ccaatggtgg tggggtatcg catgaagcct ttcaccttct ggttggcgaa
    46081 gcgtctggtg aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt
    46141 caaagagtta ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct
    46201 gttggcgaac gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca
    46261 gatccgctgc aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc
    46321 gaatttgtct atccgcacac gcaactggtt gcgggtgtgg atgaagtcgg acgcgggccg
    46381 ttagttggcg cggtcgtcac cgctgcggtg attctcgacc cggcgcgccc gattgccggg
    46441 ctgaatgatt ccaaaaagct gagcgaaaaa cgtcgtctgg cgctctgtga agagatcaaa
    46501 gagaaagcgt tgagctggag tctgggccgc gcggaacctc acgaaatcga tgaactgaat
    46561 attttacatg cgaccatgct ggcgatgcag cgtgccgttg ctgggctgca tattgcgccg
    46621 gaatatgtgt tgattgatgg taaccgctgc ccgaaattac cgatgccttc gatggcagtg
    46681 gtgaaaggcg atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg
    46741 cgcgacgccg aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac
    46801 aaagggtatc caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac
    46861 catcggcgca gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt
    46921 cgagattaag taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg
    46981 cacagcgact actcgatgat cgatggcctg gccaaaaccg caccgttggt aaaaaaggcg
    47041 gcggcgttgg gtatgccagc actggcgatc accgatttca ccaacctttg cggtctggtg
    47101 aagttctacg gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaac
    47161 gtccagtgcg acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat
    47221 accggctatc agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc
    47281 gccgggccga tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt
    47341 tccggcgggc gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta
    47401 gatgagtgtg tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc
    47461 cgcaccggca ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg
    47521 cgcggtttgc ccgtcgtggc gactaacgat gtgcgcttta tcgacagcag cgactttgac
    47581 gcacacgaaa tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg
    47641 cgtaactatt cgccgcagca atatatgcgt agcgaagagg agatgtgcga gctgtttgcc
    47701 gacatccccg aagctcttgc caacaccgtt gagatcgcca aacgttgtaa cgtaaccgtg
    47761 cgtcttggtg aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat
    47821 ctggtcaagc gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt ccctgatgag
    47881 gaagaacgtg ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt
    47941 atcaaccaga tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg
    48001 aaagataacg gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc
    48061 tacgcgctga aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc
    48121 cttaacccgg aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc
    48181 gatcaggtta tcgaacacgt agcggacatg tacggtcgtg atgcggtatc gcagattatc
    48241 accttcggta cgatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat
    48301 ccgtacggct ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg
    48361 gcgaaagcgt ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt
    48421 aaggcgctga tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac
    48481 gccggtgggg tggttatcgc gccgaccaaa attaccgatt ttgcgccgct ttattgcgat
    48541 gaagagggca aacatccggt tacccagttt gataaaagcg acgttgaata cgccggactg
    48601 gtgaagttcg acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg
    48661 atcaacaagc ggcgggcgaa gaatggcgag ccgccgctgg atatcgccgc gatcccgctg
    48721 gatgacaaga aaagcttcga catgctgcaa cgctcggaaa ccacggcggt attccagctt
    48781 gaatcgcgcg gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg
    48841 atcgcactgg tggcactgtt ccgccctggt ccgttgcagt cagggatggt ggataacttt
    48901 atcgaccgta aacatggtcg cgaagagatc tcctatccgg acgtgcagtg gcagcatgaa
    48961 agcctgaaac cggtactgga gccaacctac ggcattatcc tgtatcagga acaggtcatg
    49021 cagattgccc aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg
    49081 atgggtaaga aaaagccgga agagatggcg aagcagcgtt ctgtatttgc tgaaggtgca
    49141 gaaaagaacg gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc
    49201 gcgggttacg gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg
    49261 ttatggctga aagcgcacta tcctgcggag tttatggcgg cggtaatgac cgccgatatg
    49321 gacaacaccg agaaggtggt gggtctggtg gatgagtgct ggcggatggg gctgaaaatc
    49381 ctgccaccag atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc
    49441 gtgtatggta ttggcgcgat caaaggggtc ggtgaaggtc cgattgaggc catcatcgaa
    49501 gcccgtaata aaggcggcta cttccgcgaa ctgtttgatc tctgcgcccg aaccgacacc
    49561 aaaaagttaa accggcgagt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt
    49621 gggccacatc gcgcagcgct gatgaactcg ctgggcgatg cgttaaaagc ggcagatcaa
    49681 cacgcgaaag cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg
    49741 gaacaaattg aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat
    49801 ggggaacgtg aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa
    49861 gagattgagc gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt
    49921 aaagtcatca cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc
    49981 aatcgtatcg gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt
    50041 actgacgccc tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc
    50101 ggacaggtca gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg
    50161 gatattgacg aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg
    50221 caaattgatg accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg
    50281 acaattccag tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc
    50341 gcgacgtggc gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt
    50401 tcggagcagg tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt
    50461 gattttgaac agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc
    50521 cgtcaggatg agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa
    50581 agcgtagaac tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg
    50641 gcacgccatc cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt
    50701 gacgaactgg ctggcgaccg cgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc
    50761 cgtctcgatg gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa
    50821 aaaattcgcc gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg
    50881 atgcaaatgg ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct
    50941 tatcctggcg tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt
    51001 gaaatgtctc gcctcggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc
    51061 ggtgcgctgg cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc
    51121 gttatctcgc cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agcgccgctg
    51181 gcggctgaag cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc
    51241 atcatcccgg aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg
    51301 aaagcgcaac tgctggcgga tctggccgat ctcgacgtgt taagcactga agatttaaaa
    51361 aatcgtcgtt atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa
    51421 aagggtcact tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa
    51481 ggattttcca ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt
    51541 ataaagatga acccattaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta
    51601 tctggccaca aaacagcgtt gatttgctga agtttatcga acataaccca cgaatttgcg
    51661 gcgtgatttt tgactgggat gagtacagtc tcgatttatg tagcgatatc aatcagctta
    51721 atgaatatct cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc
    51781 aggatatgcg gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg
    51841 ccattcgtat gcgtcagtat accaacgaat atcttgataa cattacccca ccgttcacga
    51901 aagccttgtt tacctacgtc aaagagcgga agtacacctt ttgtacgccg gggcatatgg
    51961 gcggcaccgc atatcaaaaa agcccggttg gctgtctgtt ttatgatttt ttcggcggga
    52021 atacccttaa ggctgatgtc tctatttcgg tcaccgagct tggttcgttg ctcgaccaca
    52081 ccgggccaca cctggaagcg gaagagtaca tcgcgcggac tttcggcgcg gaacagagtt
    52141 atatcgttac caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat
    52201 ccggcagtac gctgttgatc gaccgcaact gccataaatc actggcgcat ctgttgatga
    52261 tgaacgatgt agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attctgggtg
    52321 ggatcccgcg ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccaccacgc
    52381 aagcacaatg gccggttcat gcggtgatca ccaactccac ctatgatggc ttgctctaca
    52441 acaccgactg gatcaaacag acgctggatg tcccgtcgat tcacttcgat tctgcctggg
    52501 tgccgtacac ccattttcat ccgatctacc agggtaaaag tggtatgagc ggtgagcgtg
    52561 ttgcgggaaa agtgatcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc
    52621 aggcttcgct gatccacatt aaaggtgagt atgacgaaga ggcgtttaac gaagccttta
    52681 tgatgcatac caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg
    52741 cgatgctgcg tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc
    52801 attttcgcaa agaggtccag cggttgcggg aagagtctga cggttggttt ttcgatatct
    52861 ggcaaccgcc gcaggtggat gaagccgaat gctggcccgt tgcgcctggc gaagagtggc
    52921 acggctttaa cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt
    52981 tgacaccggg gatggacgaa cagggcaata tgagcgagga ggggatcccg gcggcgctgg
    53041 tggcaaaatt cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc
    53101 tgtttctctt tagtattggc atcgataaaa ccaaagcaat gggattattg cgtgggttga
    53161 cggaatttaa gcgctcttac gatctcaacc tgcggatcaa aaatatgctg cccgatctct
    53221 atgctgaaga tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc
    53281 ataagctaat tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc gatactttgc
    53341 cggaaatgat catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa
    53401 ccattgcgct ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatcctc
    53461 ctggcgtacc gctgttgatg cctggagaaa tgctgaccaa agagagccgc acggtactcg
    53521 attttctact gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc
    53581 acggcgcgaa acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat
    53641 aacttgccag agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat
    53701 gctgggttta aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa
    53761 agctttctac tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg
    53821 cgactcgtgg aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcatt
    53881 tccgttcccg ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt
    53941 tagcgttgat gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga
    54001 agccatccgt gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg
    54061 gctgccgttg gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt
    54121 gccgggcatt gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc
    54181 tcacctcacg ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc
    54241 atcagttggt gcagtggcgg acggaaaatc cgggtgtcgc tctgcgcgct attcatgtgc
    54301 atcacggttt aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac
    54361 agtggcaggt gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg
    54421 aggcccaggc gcggcaggcg cgttatcagg tatttgcccg caccttgttg cccggtgaag
    54481 tgctggtcac cgcgcaacat ctcgacgatc aatgtgaaac ttttctgctg gcgctaaaac
    54541 gcggtagcgg ccctgccggg ctttcggcta tggcggaagt ttcggagttt gccggaacac
    54601 ggcttatccg cccgttgctt gcccgcacgc ggggggaact ggcgcagtgg gcattggcgc
    54661 atggtttact ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc
    54721 tgcgcctgcg cgtagtgccg ttattgcagc agcgttggcc gcattttgcc gaaacaacgg
    54781 cccgcagcgc cgcactttgt gctgaacaag agagcctgct ggatgaactg ctggcagata
    54841 atttagcaca ctgtcaatcg ccgcagggga cgctgcagat tgtgccaatg ctggcgatga
    54901 gtgatgcccg ccgcgcggcg attatccgcc gctggctggc agggcagaat gcaccgatgc
    54961 cttcccgcga cgcgttggtg aggatctggc aggaagtggc gctggcgcgg gaagatgcct
    55021 ccccctgttt acgtttgggc gcgtttgaaa tccgacgcta tcagtcgcaa ctgtggtgga
    55081 ttaaatccgt caccgggcaa agcgaaaaca ttgtgccgtg gcagacgtgg cttcaaccgc
    55141 tggaattacc ggcggggctg ggaagtgtac agcttaatgc gggaggtgct attcgccctc
    55201 cgcgtgcaga cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg
    55261 ggcgtaacgg cggacgtaag ctaaagaaaa tctggcaaga gctgggcgtg ccgccgtggc
    55321 tacgtgacac cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat
    55381 ttgtgacgca agaaggtgtg gctgaaggtg agaatggcat aagttttgtc tggcagcgaa
    55441 acgcttagtt aagtgaaggc cggatatgac gcattaagcg tcgcatccgg cgaaagtcaa
    55501 tcaggactcg ctcaccacca ccgtaccgat ttccgggtgg ctaaagctgg taattttatc
    55561 cagacgtagc tcacgggttt cgccagcggc ctcgacgacc agatactcca catttttgcg
    55621 ggagactaaa tcgctggctt ttgcctgcaa tttttcgcca tctttcagct caagtgtcag
    55681 cattaaatga tgctggcagg cgagctcaag attatcgtaa tcatcacaat tgattggttg
    55741 atacgtatca ttcattgaca taatcgctca ccagtaagtt tgccgcagcg tatgctgctt
    55801 tttccctgac agcctcagaa agggcgtcgt cggcagccat ttcattcagc actttcaaaa
    55861 cgcagcccag cgcgtccgga acgtatccta agtctccgct ggcgatttcc gcgtaccgct
    55921 tgcgtattaa ctcacaatat ttttccacat gccctcctgt cagcactctg acttaaccgt
    55981 ggatgcaagt ctaagcctac gaagataaac tctgtttcgc aaggtgacta taccacactc
    56041 atttctgcaa tatcagcgcc gcaactgcac gtattccgtt acaatggcct cctgattcga
    56101 aaggagtttt cttatggcgc ttaaagcgac aatttataaa gcgacggtta atgtggccga
    56161 tctcgaccgc aaccagtttc tcgatgcctc tctgacgctg gcgcgccatc cttcagaaac
    56221 ccaggagcgt atgatgctgc gcttgctggc gtggctgaaa tatgctgatg aacgtctgca
    56281 atttacccgt ggtttgtgtg ccgatgatga gccggaagcg tggctgcgta acgatcacct
    56341 gggcattgat ttgtggattg agctggggct gccggatgag cggcggatta agaaagcctg
    56401 cacccaggcc gcagaagtgg cgctgtttac ctataatagt cgggcggcgc aaatctggtg
    56461 gcagcaaaat cagagcaaat gtgtgcagtt tgccaatctt tccgtctggt atctggacga
    56521 tgaacaactg gcgaaagtaa gcgcctttgc cgatcgtacc atgacgctgc aggcaacgat
    56581 tcaggatggc gtgatctggt tatcggatga taagaataat ctggaagtga acttaaccgc
    56641 ctggcaacaa ccttcatgat tgtgatttcc cgacatgttg ctattcccga tggtgagctt
    56701 gagatcaccg ccattcgtgc gcagggcgcg ggcgggcagc atgttaataa gacctcaacg
    56761 gctattcatc tgcgttttga cattcgggcg tccagcctgc cagagtatta caaagagcat
    56821 ctgctcgccg ccagccatca tttgatcagc agtgatggcg tgattgtcat taaggcacag
    56881 gaataccgca gtcaggaact gaaccgcgaa gcagctctgg cccggctggt ggctgtgatt
    56941 aaagaattaa caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa
    57001 gagcgcaggc tggcatcgaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagtg
    57061 cgcagcggtc gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat
    57121 ggctgtaatc agcctcttta ctctgatggg ttgtaataat cgggccgaag tcgatacgct
    57181 ttctccggcg caggctgccg aattgaaacc gatgccgcaa agttggcgcg gcgtgctgcc
    57241 gtgtgccgat tgcgaaggaa tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt
    57301 gatgaatgag cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac
    57361 atgggcgcga accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta
    57421 tcgggcgaaa ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccga ttgaatcgca
    57481 gttcaactat acgctggaag cggcacaatc cagtttaccc atgacgccga tgaccctgcg
    57541 gggcatgtat ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg
    57601 tttcatggta gcgaataacg cagagctgga gcgtggctac ctggctgcgc gcggtcacag
    57661 tgaaaaaccg gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac
    57721 cggtgcgcca accaaagtat tagcacccga tacggcaggg aaattttacc caaaccagga
    57781 ttgcagtagt ttggggcagt aacccgtctt gagacagaaa caaacgcaaa acaggccaga
    57841 aggatatatt tcaacatttt gaatttgcac gttttttgta ggccggataa ggcgtttacg
    57901 ccgcatccgg caatggtgct caacgcctga tgcgacgctg gcgcgtctta tcatgcctac
    57961 aacccccctc ataccttaag gcggataagg catttacttt atcaccgggt cagcccaaca
    58021 aagcgacccg ccttgatata aatccctggc ttcaggtgcc caacatactg taactgcgtt
    58081 tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc
    58141 cagcccgatt gcgccagcgt aagcggatgc ccggctttgg caaatgcatc agtgacaaat
    58201 tccgaacaaa accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc
    58261 aggccgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg
    58321 cgagtcacca taaaggggat aaattcgaca atgccgcgat agttataacc gctatctttg
    58381 attttattgg cgaacgcggt gatttctgtg gcttgttgcg gggtaagatc cgggactcgt
    58441 aagacgaaaa gtttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca
    58501 gcgcctgtcg cttctgcaac gttattttca cccagataga ttgcaacgtg actcacagag
    58561 gaggtgctga aaacgcggat cccaaatgag gttaccccca ggcttgagga gaacagcaaa
    58621 tcgctgggtt tgagatcagg taccgttatt tcttttattg attgttcggt gaaagagctt
    58681 tgatgctgga atttgacagc ccatgttttt gcctcaacat ctaccgctgt ggcagaggag
    58741 tctggctggc tgatatcaac ggtacaggcc gaaagtaaaa gaaagcaggg gagaagcagg
    58801 cggtagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg gccctgaaat
    58861 tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga cgatgtcacc
    58921 agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat cgtcgttgtc
    58981 gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca tatcggcaaa
    59041 catcacgccc ggacgttctt ttcggtcatc cagcagcact tcgatacctt gtgcacgcag
    59101 ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca tgttcatcgg
    59161 cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc gttcgtcgta
    59221 gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc aacccatcgt
    59281 caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt cggagtactt
    59341 ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca gcgtaccctg
    59401 gccatccggg cttggatcgc cagccaccac gttgcggata tcagcaattt ccggggtagc
    59461 gacatcgcga tcccagttga tgccgaagta gtgtttacca tcgatgttag caccagcagc
    59521 gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca tgtttaccgg
    59581 acccagtgaa cccggaccgg cgttaaccac ggcacgaatt tcttcttcgg tcgcgaaagt
    59641 cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagtt cgtggtcacc
    59701 gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa ccagcagagt
    59761 cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga tggttttcgc
    59821 gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc
    59881 gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga agaccacatc
    59941 gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc
    60001 ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc
    60061 cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga aagagtaagc
    60121 atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg
    60181 gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc taagctcgtt
    60241 acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg gacgctcgcc
    60301 acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac tctcttgcca
    60361 caaatcggct ggctgaacca ccggcatcga cacctcgatt gcaccggcgt tgttcatctc
    60421 ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca gccaggtata
    60481 taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat ggctgatcac
    60541 ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag tacgcatgtt
    60601 gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca aaaaaagtga
    60661 tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta acgcggttcc
    60721 agtgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa atcatgcagc
    60781 cagacggcat aggttttgcc cgtttcctca cctttacgat aggctgggcg cgggtcctgc
    60841 gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag cgtcaaaagc
    60901 tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc actttgcgca
    60961 tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt gatatccact
    61021 accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct gtctttatgg
    61081 caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg gaaagtagag
    61141 cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg cacagtcgga
    61201 cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg gctgaacgct
    61261 tccaggccgc gaacggcgtc ggcctggttg tagggagcaa tgagatgcag ttcgccgttg
    61321 gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata gggcgagcga
    61381 ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta agcgcagagc
    61441 cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg cagctatgca
    61501 gtaatacagc attggctttc attttagagg cgttgatttg catccgctta cgtgcggttg
    61561 gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg acttcaccga
    61621 gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg accggcgtgg
    61681 cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg ctttgaacgg
    61741 gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct actaaacaga
    61801 tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata ttgaatcagg
    61861 agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac aaattgtgga
    61921 aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg tttgctgctt
    61981 cgtaaacttc gtcagactga taagcctgaa cgaatttctt cacgttctcg gcgtctttgt
    62041 tatcttcacg cgttacgatc aggtttacgt acggggactc tttatcttcg acaaagatac
    62101 cgtctttcgc cggagtcagg ccaatctggc tggcataggt ggtattgata actgccagag
    62161 cgatttgcgc gtcgtccaga gaacgcggca gctgcggtgc ttccagctca acaattttca
    62221 gatttttggg gttctcaaca acatcaagaa cggtcggcag caggccaacg ccatctttca
    62281 gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaaggtta gttgggtcgt
    62341 ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt ttcttggagt
    62401 aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag ccacgatctt
    62461 tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata tcgcctttgc
    62521 tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg tccaggccat
    62581 atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca ccaacaatca
    62641 cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc agtgccagtg
    62701 atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata ccttattcct
    62761 tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg acgatgcggt
    62821 cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc gtattcatca
    62881 ccgtcgcgtt atagccgatg tagccatact gatagccaat ctgacctaaa ccaccggcac
    62941 cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata gttgccgcat
    63001 tcaccagacc cggcaacgct tccggtaaca gcactttacg gacgatctgc attggcgtgg
    63061 cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga gcgttctcga
    63121 ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct gcctgcaaac
    63181 caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc aagataatga
    63241 acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc ttcgcgttag
    63301 caataatttg ccccggacgc gtgacataaa gcaggacgcc aaccggcaga ccaatcacaa
    63361 acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca cgaaccagca
    63421 gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg ttcctgcagc
    63481 caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag catgatgccg
    63541 aacttaacgc caccggcgta atccatctgc gcgctaataa tgttgttgtt gacgttgaaa
    63601 cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa ctccagacgc
    63661 agcatcggca cgcagtcagt aaatggctcc gcttgcagac gttcctggta atcttccggg
    63721 atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt cggatgcgag
    63781 aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tactgataac cgccacgcaa
    63841 tcacaaatgc gcttcacaac gtccatttcg tgggtgatca acagaatcgt caaacccaga
    63901 cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg gtctagcgcg
    63961 ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg ggcaatcgcc
    64021 acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt atctccaaga
    64081 ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg tgtattgtcc
    64141 agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag gttaaaatgc
    64201 tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga cagcgtggtc
    64261 agttcctggc catcgaccag cacactaccc tcggttgggc gctccagcag gtttacacaa
    64321 cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat ttgcccagct
    64381 ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg gtggaacact
    64441 ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag ttagtcgtgg
    64501 catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag gcatccagac
    64561 gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac atgcgatact
    64621 agcgtcacat gccttattaa ggagctataa aaggtggcga agagcgtacc cgcaattttt
    64681 cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat cgacaacttt
    64741 gaatttatcg acggtgttat tgacgccatg cgcgagctta aaaaaatggg ctttgcgctg
    64801 gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc acagtttgaa
    64861 acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct ggatggtatc
    64921 tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaggt ctgcgattgc
    64981 cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat tgatatggcc
    65041 gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc ggcgaacgtg
    65101 ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga aaacgcggca
    65161 gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca gcaaaaaccg
    65221 gcgcaatgat taaaagatga gcggttgaaa taaaaatgca tttttccgct tgtcttcctg
    65281 agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    65341 agctggttcg gt
//
LOCUS       DAPGRN010000013        61657 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPGRN010000013 DAPGRN010000000
VERSION     DAPGRN010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.013"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..4555
                     /gene="sslE"
                     /locus_tag="RHW33_000922"
     CDS             <1..4555
                     /gene="sslE"
                     /locus_tag="RHW33_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HDV1644463.1"
                     /translation="KKFKYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLP
                     EVKPDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSQRVTG
                     ATCNGESSDGFTFKPGEDVTCVAGNTTIATFNTQSEAARSLRAVEKVSFSLEDAQELA
                     ASDNKKSNAVSLVTSSNSCPADTEQVCLTFSSVIESKRFDSLYKQIDLAPEEFKKLVN
                     EEVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGR
                     LVDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIAL
                     TELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGAT
                     LGEGEQVVNLPNEFIEQFNTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGV
                     INKLWGVDEDYKSVTKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYW
                     LAFGEKRAWDKNELAYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPH
                     YNSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTV
                     GTNLETVYFKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYV
                     TQWGSDPYSIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFV
                     RLLDAAGLSMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTK
                     EVIWKYQQENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKKRILD
                     AFPGLEECKDSDYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADL
                     GTNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGF
                     KTFTEFLNCYTNDAYTDGTQCSAELKQSLIDNNMIYGDGSSKAGMMNPSYPLNYMEKP
                     LTRLMLGRSWWDLNIKVDVEKYPGAVSVGGEEVTETISLYSNPTKWFAGNMQSTGLWA
                     PAQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKV
                     PYGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTT
                     PKKNLEASNYKGGLKQFAEELDTFASSMNDFYGRDGENGKHRMFTYKALTGHKHRFAN
                     DVQISIGDAHSGYPVMNSSFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEV
                     ANNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWA
                     EKNFDIKKWYPEGELPKFYSEREGMKGWNLFQLMHRKARGDDVGKTKFGERNYCAESN
                     GNAADTLMLCASWVAQTDLSAFFKKWNPGANAYQLPGATEMSFEGGVSQSAYETLAAL
                     GLPKPQQGPETINQVTEHKMSAE"
     gene            4882..5691
                     /gene="pppA"
                     /locus_tag="RHW33_000923"
     CDS             4882..5691
                     /gene="pppA"
                     /locus_tag="RHW33_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HDV1644464.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            5757..6167
                     /gene="gspS2"
                     /locus_tag="RHW33_000924"
     CDS             5757..6167
                     /gene="gspS2"
                     /locus_tag="RHW33_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HDV1644465.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCAGHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     LTEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            6185..7144
                     /gene="gspC"
                     /locus_tag="RHW33_000925"
     CDS             6185..7144
                     /gene="gspC"
                     /locus_tag="RHW33_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HDV1644466.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIARGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTVVSPSVNKPPRTDAKTFDKNDVQLISQQNWF
                     GKYQPVAAPVKQPEPAPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLVEEERSTVAVTNKKAISDEVKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            7174..9234
                     /gene="gspD"
                     /locus_tag="RHW33_000926"
     CDS             7174..9234
                     /gene="gspD"
                     /locus_tag="RHW33_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HDV1644467.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            9234..10727
                     /gene="gspE"
                     /locus_tag="RHW33_000927"
     CDS             9234..10727
                     /gene="gspE"
                     /locus_tag="RHW33_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HDV1644468.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVRAGETSLDEVLRVTMEA"
     gene            10727..11950
                     /gene="gspF"
                     /locus_tag="RHW33_000928"
     CDS             10727..11950
                     /gene="gspF"
                     /locus_tag="RHW33_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HDV1644469.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIET
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPATTRALIAM
                     SDTLQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            11967..12422
                     /gene="gspG"
                     /locus_tag="RHW33_000929"
     CDS             11967..12422
                     /gene="gspG"
                     /locus_tag="RHW33_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HDV1644470.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGVFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            12426..12989
                     /gene="gspH"
                     /locus_tag="RHW33_000930"
     CDS             12426..12989
                     /gene="gspH"
                     /locus_tag="RHW33_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HDV1644471.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGMVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPCTLRFYS
                     AAQNACWAVKLAHDGALSLSQCDERMP"
     gene            12986..13357
                     /gene="gspI"
                     /locus_tag="RHW33_000931"
     CDS             12986..13357
                     /gene="gspI"
                     /locus_tag="RHW33_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HDV1644472.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTTPQNTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            13354..13959
                     /gene="gspJ"
                     /locus_tag="RHW33_000932"
     CDS             13354..13959
                     /gene="gspJ"
                     /locus_tag="RHW33_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HDV1644473.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVTGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            13956..14933
                     /gene="gspK"
                     /locus_tag="RHW33_000933"
     CDS             13956..14933
                     /gene="gspK"
                     /locus_tag="RHW33_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HDV1644474.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNVLAQPTTASRPLALQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            14930..16108
                     /gene="gspL"
                     /locus_tag="RHW33_000934"
     CDS             14930..16108
                     /gene="gspL"
                     /locus_tag="RHW33_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV1644475.1"
                     /translation="MSSILEIFFPLCASDTIRWQRRTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITCATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRVLAPVMFRAQLNEQDLPLSLTVVGIAPEELSAWAGADAERLTVTVLPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSHAQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQTDDTDLLAELSAIASTLKTASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRTALAADFIVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            16110..16646
                     /gene="yghD"
                     /locus_tag="RHW33_000935"
     CDS             16110..16646
                     /gene="yghD"
                     /locus_tag="RHW33_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV1644476.1"
                     /translation="MLRDKFIHYFQQWRERQLSRSEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLKWLKALDEKYALRVTQIDVS
                     AAEKLGMVNVQRLEFGRG"
     gene            complement(17002..18101)
                     /locus_tag="RHW33_000936"
     CDS             complement(join(17002..17846,17846..18101))
                     /locus_tag="RHW33_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV1644477.1"
                     /translation="MKKTRYTEEQIAFALKQAETGTRVGEVCRKMGISEATFYNWKKK
                     FAGLGVTELRRLRQLEDENQRLKKLVADLSLDKEMLQEVLKPKVLRPAQKRQAVHFLR
                     EAYRISVRRGCGLLIQSRTVYHWQSRRDDRAITLRIREIAETRIRYGCPRIHIQLRQE
                     GWPVNHKKTHRIYCLEGLNLRRKRPCRHVSAAHRQQRPILTGVDQCWSMDFVSDNLFN
                     GRRFRALTVVDNFSRECLAIHAGKSLKGEDVVGVMEGLRVPGKRLPVRIQTDNGSEFI
                     SKSLDKWAYEHGVTMDFSRPGKPTDNPFIESFNGSLRDECLNIHWFLSLEDAQDKLDS
                     WRREYNHERTHSSLNDMTPAEFIRSLRKDEDL"
     gene            complement(18230..20461)
                     /locus_tag="RHW33_000937"
     CDS             complement(18230..20461)
                     /locus_tag="RHW33_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644478.1"
                     /translation="MIPNLNELTDTPIARTNLIKLEEDQLTTIQRLLAPVSNIYTIDF
                     MVQHFTKERKEKSADYYARIHQEVKTCVRQKLGLEAGQEVKYELHCLPNYHHVFFFLA
                     PAAAPNSLAHRTLAERIETLCQRLTAENYDLSRLIQGLFSLHLKMVMLEQASERFSVP
                     PTYFNSTFYLNARLSQPVTQKSGTGVMEAFELDIYASEYNELAFTLHKRKFLVEPEDE
                     LHLSLDDTCVWFNIDNRRLKARRKLDARDSKLDFFRERSGYGECQAYTYNVVMNAACE
                     RLSELEIPHQPIAFQATHEVNQFATDLDQQLTNTLLVVNNGVEFSATQEAYFFDTLAI
                     QFPGYQLWPLASLKHSQQTGFSELPASTSILVLNAVDEERSNSIRQQDNESVEYNDFY
                     AAFADARKQPELNWDTYTQLKLDRLQGWLNQQPLPVVLQGMNIDHKLLDAIDFINEQL
                     TSNPTQYEIDLTKPHSRLKSAVTLLNSKVRRTKTELWFKESLLNQHHIPLPDLADGHY
                     TAYAVRKTKSYLPLLGYVELKIEHGQLRVVDTGIAEGKLDYLSVDPPSLGRLKKLFDK
                     SFYLYDHTADVLLTTYNSSRVPRLIGPAQFNIVDSYAYQEQEKTLAERKGDKFNGYAI
                     TRSAKPDQNVLPYLISPGRSKYDSLTKAQKMKHHHIYLQPHENGVFVLVSDAQPTNPT
                     IARPNLVENLLIWDAQGKAVDVFSHPLTGVYLNSFTLDMLRSGESSKCSIFAKLARLM
                     VEN"
     gene            complement(20458..25173)
                     /locus_tag="RHW33_000938"
     CDS             complement(20458..25173)
                     /locus_tag="RHW33_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644479.1"
                     /translation="MRKSWTIEEDCKLLTLVRQLFSALVSHNRLNATMPFSQQLHDAF
                     DSPDRDAAALLYRLEQAKILGFASRPGGDPTKQLFRCLISNDLALYDYSLTFPTLRKA
                     LHPDTVAAALNHFTISNPHEPLSNTINEIATALHLAPMQVEKILIDSGQITINSYRKC
                     ERVGEKNINNNLQDLISRQIPDITLIKEINACRAQVSQLYHVHERDGAEVIFSSDGTG
                     FGKSYGVIQGYVEYLERFAKTQKSDDLFPEGGFTNLLFMSPQKSQIDLDSSQKEKILA
                     ASGEFICVLSRKDVADLDFMDWASGLKNRDRYIQWYEGAKGSKYIGGAMRSLNYHVLQ
                     IDRCEEQLKKLTTYGSQDTNYEREILEEQLKNCRHSIRNTIESACKLLFGPDSEKASI
                     KEYIRRGLQARQERMQNAETARKPGKLEPKISVHEVYFELIKQVLPFEVCQYRPSVLL
                     MTTNKFDTSTYRLAPRQRGEGVRFESVGFDLLIGGKLTPKDPQISTVAAAGHTGQVTY
                     LRDEHFRRNPDCPFRQKNIRFTVIIDELHEAYTRLEETCHVKLITQENNLAHVISVAG
                     RIHNALLSLERRNKPKEAQTTFEQEMVKFITTLRNLLAEKCELSPGTRLGSILEMFRD
                     QLGAFEVNGDAAERIISITRNVFSFNPKMYVNEEGLKRIRMRNSEGDITRTELYYEVE
                     NDANDTNPTLHDLFQLVSVILAACSEITNRHFKRWVKNGGQDNSSSQNTPLGQFVDAA
                     NNVAGVVRHIFDRTTDKNLLIDHFYTYLQPKTVFTMTPIAELNYVNRGAERTIILAFE
                     MDLVQELPEAMLLRLLTGTHNKVIGLSATSGFSHTKNGNFNRHFLAHYSRDLGYRVVE
                     REKADIDTLKALRGLRASIRNVDFRVFDDKQLKLTDIYQNCEIYRRTYDNFFDALKKP
                     LEYDLKNTYKRRQCQRELEALLLAAWEGKNSLILSLSGTFKRAFISAWRTHQTTWRQQ
                     YGMHSRCDEKTDNGKKHDQILTFTPFKGRHTVHLVFFDSPLANVEDIRQETYLQNSNT
                     VLVFMSSYKSAGTGLNYFVKYHDGDINDINAPRLDVDFERLVLINSSFYSEVKDNSGN
                     LNTLPNYVTVLKHYADDDITVHKLADFNVNFAHGENYRLLMAEHDMSLFKVVVQAVGR
                     VERRDTLLKTEIFLPRDVFRNVAFQFAALSEDSGNEVVSESMSLLNHRLMEECEKLSQ
                     GQSFNNAEQRLTFEQAIVANGRRIDEIHKRVLKTDWINKVRAGNLDYLEICNLFRDPD
                     SFTDPQRWLAKLQANPLYTANRQMQSVHDALFIDRQQGNQTILLCHKRGPDGLAHRDY
                     SALSDFAGGAREYRPELTLFPQYRNDVDFTPGNLVGELIRECDNIQEKAFKKWVPNPR
                     LVPLLKGNVGEYLFDKVLKSYGVTPLSDQQVFERLEPLVYEFFDRFIEVGDDLLCIDV
                     KRWATQLDDLTRAEETLEKSDNKIRQIRNIASQKADTEGQKQLQTALAGRYERIRFIY
                     LNVAYSQNPNNLMWQDNVDHTIHYLNLLQTDYQYYQPKNRESGRAQENSKLRMTLDIN
                     PMLLTLLGVEKLPTKGKVS"
     gene            complement(25185..27383)
                     /locus_tag="RHW33_000939"
     CDS             complement(25185..27383)
                     /locus_tag="RHW33_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1644480.1"
                     /translation="MMNEYQRLDPRIQKWIYRQGWADLRELQKKSVSPILAGDRDVLI
                     SAATAAGKTEAFFLPACSAIADIQGGFGILYISPLKALINDQYRRLENLGDALEMPVT
                     PWHGDVAQSKKLKAKKNPAGILLITPESLEAMLIRNAGWLKQAFAPLAYIAIDEFHAF
                     IGSERGMQLLSLLNRVDHLLGRINNPVPRVALSATLGELEQVPLSLRPNQRLPCDIIT
                     DSQTHATLKVQVKGYLEPLTTSGQQSPPSAETQICHDIFRLCRGDSHLVFANSRKRTE
                     SIAATLSDLSEASIVPNEFFPHHGSLSRDLRETLEQRLQQGNLPTTAICTMTLELGID
                     IGKVSSVVQVTAPHSVASLRQRMGRSGRRDSPAVLRMLIAEHELTPTSGIVDQLRLQL
                     VQSLAMIRLLIGNKWFEPADTRQMHYSTLFHQILAIVAQWGGVRADQIWSQLCLQGPF
                     QKVRIYDFKTLLKHMGEHQFLTQLSSGELVLGVEGERQVNQYTFYAVFSTPEEFRIVA
                     GSKTLGSIPVDSPLMPDQHIIFGGRRWKVTDIDSDKKVIYVEATKGGQPPLFGGQGMS
                     IHDVVRQEMLTIYREGDYRITVGNRKADFADTTAKNLFDEGLHCFRNNNLASECFIQQ
                     RQHVYILPWLGDQTVNTLSALLIQRGFKAGSFAGVVEVEKTTVSEVKQALFSALQEGL
                     PYESRLAESIVEKCLEKYDEYLPETLLTQEYGLRAFNIERVTEWLQGHLY"
     gene            complement(27380..28696)
                     /locus_tag="RHW33_000940"
     CDS             complement(27380..28696)
                     /locus_tag="RHW33_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV1644481.1"
                     /translation="MSGIRIRLKERDAIIQSLKSGVTPKIGIQHIQVGRVNEIKALYQ
                     DIERIADGGAGFRLIIGEYGSGKTFFLSVVRSIALEKKLVTISADLSPDRRIHATGGQ
                     ARNLYSELMKNLSTRNKPDGNALLSVVERFITEARKEAESTNVSVPTIIHQKLAALSD
                     MVGGYDFAKVIECYWQGHEQDNETLKSNAIRWLRGEYTTKTDAHNDLGVRTIISDASF
                     YDSLKLMSLFVRQAGYAGLLVNLDEMVNLYKLSNTQARVANYEQILRILNDCLQGTAE
                     YIGFLLGGTPEFLFDPRKGLYSYEALQSRLAENSFAQRAGVIDYSSPSLHLASLTPEE
                     LYILLKNLRHVYSGGDADKYLVPDDALTAFLRHCSNTIGDAYFRTPRNTIKAFLDMLA
                     VLEQNPSIQWSQLIAGVAIAEEKPSDMDEITSAEDADEDGLADFRL"
     gene            complement(28700..31027)
                     /locus_tag="RHW33_000941"
     CDS             complement(28700..31027)
                     /locus_tag="RHW33_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB N-terminal domain-containing protein"
                     /protein_id="HDV1644482.1"
                     /translation="MGFWIFVIMLAVIWLLFSKKKKSPPPRVNNKIITKINHSSRQKS
                     LNKPDNSMTNMHSQASDDDELATFTFVNGQTVEYSTSRQPSRENAARSNTTPARWVKP
                     GESITIQNVVINHGYFYFGGRLKTHSSGEYGYLYNDDSDASLVNDAFPIEPGSRHYYD
                     ESLGYWPSFATLSPRCRGAYLDWLASDRSDASCPVGYVFIYFYGLERRVLADGTQEAI
                     SDDEFKALFEEISRLRTVFQASGSFRHYATQLLEMMIVLRPKLLSIYTENEYFSSRSS
                     LLFRLNLATVVDKGQPICAALALAWIYYFPDYTLRTPARRCHAEFSALFKQRYTQKYG
                     DGIVVKPNKTRLYLSYTPASGTLRELQVKKQMDLPDPSVLKAPVQKLISVAESCINAL
                     DAYSRYLGKKDASPSDVAAIMLLPDEILTEDAERLFAEFKHWADEKIREHSGLATVAD
                     FWARLGMPVPDKINKKEAELMQNFARRAGYGIAPDMRYHLVRPDPEGHLVLFPEGHAE
                     FYVPSAEFTSVSVALRLGAMIAQMDKRVDVAEQAALEKTINHNDALSPTEKRSLHAYL
                     TWRLNTPANQAGLKGKIEQLSDKDKSTIGNVIISVACADGKIDPAEIKQLEKIYASLG
                     LDSSAVTSDIHRLSTAETTPTATLQTPSATSGAFSLDERILARHESDTTDVRQLLNTI
                     FTEDEPADESPAEIPPHAGAGLDEAHHQLYQRLQEKERWARNEVAELCQQFNLMLSGA
                     IEAINDWSFEQVDAPVLDDDDDIYVDLEIAQELKG"
     gene            complement(31211..32278)
                     /locus_tag="RHW33_000942"
     CDS             complement(31211..32278)
                     /locus_tag="RHW33_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDV1644483.1"
                     /translation="MDIFDYYRSASQRRELQEILSYAAQQHPSGLGASFLEKDLWVTE
                     ILRLLFNEELLGGLSVAFKGGTALSKCWNVIERFSEDIDLSVHWADLAGHSEEEEQQA
                     WEQSTLNNSQNRRFREQQQERLTEWTNALAERLNQRFQAYEIPGLEAKMEPDSGGEKV
                     DIYFPRVKEDSMAYQRDHILLEFGGRNRGKPTDLMPVVSYLSGIAGMDTLQLPTATVN
                     AYDTGYILWEKLTALHQFCTQTKAPNPARLARHWYDVDCLLSNHFASPYETLEAMRNV
                     VEMKQRRWSVPGVDFTQVIVGNLQLLPDDAERFAAIAGDHQTAVEGGMFFHQPDRFAD
                     IADRLRHAQDEINRFMRDSEV"
     gene            complement(32278..32895)
                     /locus_tag="RHW33_000943"
     CDS             complement(32278..32895)
                     /locus_tag="RHW33_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644484.1"
                     /translation="MSVAALARQRVHRMKRGIPFSISGFYSLGSRASVQQAMSRLVKE
                     GLLVRVERGFYSRPKPLKNIPSVNVVASANAVAAAWAKERGYKITLQGLEAAYRLGLQ
                     TQAPVKRIYWSNGPTREFRVGNERVRIQHKSASRLRWLNRPEGELMRGLLSLDQQHTP
                     LETLKNAFHRLSIPSAKARQIAGKLMNEPALHAWRSQLITLQKAL"
     gene            33094..35289
                     /locus_tag="RHW33_000944"
     CDS             33094..35289
                     /locus_tag="RHW33_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1644485.1"
                     /translation="MSILSIHGVRSYQGERPVEFDLSKYVTLIYGQNGSGKSTVSGYF
                     YKHGRPDYSQCSLRPPLDMNYLVFNQEYVDDIFSQPSQPGIFTLNSENAEIKTEIDAL
                     EAESKVLFARRDALDVQKRDVEGMEESIKNGSAKQIYSSTAEIRKTDLWDLMSGTKQT
                     DKLFQTILEHTEVEDTSTQELTEELHRLEASKGNPYALLEALPASPLNDNDIALLMQP
                     LIPAGDSRLAAAINQLGNIDWVRNGQQWLSDDICPFCQTPIDARQLQQEITALFDTSW
                     EAAMDQLRELQARYQFWHDKPEHMRQLIKTCPLVDQEHPVYLYLLELEQAYQRNKLHI
                     DEKLTSPSASIAVEDLSALAGNVSVQIASINTIISEHNRKAENYQTERVRLKQRLLSH
                     IRKLATDTIINHDEQLAELAEKLAKLTVSRDEITAQLDTLNAIIRGKSSLIVNTQETI
                     ERINHSLDSLGITGFRIAPYDDRDDYRLVREGENSDTPVFSSLSEGEKTLIAFLYFLE
                     TCTGRKSRDDNDQRKRLIVIDDPISSLSQDYVFEIASLIQHQVIRARIGEKVIILTHS
                     LFFFQELLLSAERKKRAAGSCPPEWTLYRVSKSLHSSASLVSEKELLNDYQALWYVLR
                     HAQKDDIASVVIPNTMRQILEYYFGFSGKSETLHRALETLASGPEGEPGFRIFARYLN
                     RHSHQDARNISLHEGASVERYLTWFKKVFEAAKDEEHYTSMMEKTTQTT"
     gene            36808..37689
                     /locus_tag="RHW33_000945"
     CDS             36808..37689
                     /locus_tag="RHW33_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HDV1644486.1"
                     /translation="MGMLEREMKNLARQAGGSHKTVHDRIAMAGRFCERLMELNVQIR
                     YVHHLKAKHIEAYIQMRLAQGIQKNTLHNETAAIRKILTQAGREKLAQSERISNKSLG
                     LAGVSRNGTRKAITPEYYQQMAEAARLKDAGLAAALELTRLMGLRSQEAVRCSGSLKT
                     WREALEKGDTRLTVVLGTKGGRPRDTIIQDTGAVKQALDKAIVVAERRNGRLIDAASL
                     KQAMKYWRNQTLRMGLTGKYSPHSLRYAWAQDDIRRYLAQGFSEKEALAMVAMDLGHG
                     DGRGRWVKQVYARNDEA"
     gene            complement(37871..39138)
                     /locus_tag="RHW33_000946"
                     /pseudo
     CDS             complement(37871..39138)
                     /locus_tag="RHW33_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010301506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(39300..39375)
                     /locus_tag="RHW33_000947"
     tRNA            complement(39300..39375)
                     /locus_tag="RHW33_000947"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39340..39342),aa:Phe,seq:gaa)
     gene            complement(39481..40188)
                     /locus_tag="RHW33_000948"
     CDS             complement(39481..40188)
                     /locus_tag="RHW33_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV1644487.1"
                     /translation="MVIGPFINASAVLLGGLLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            40586..42721
                     /gene="speC"
                     /locus_tag="RHW33_000949"
     CDS             40586..42721
                     /gene="speC"
                     /locus_tag="RHW33_000949"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV1644488.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILTLLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYELFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGDVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(42771..44027)
                     /gene="nupG"
                     /locus_tag="RHW33_000950"
     CDS             complement(42771..44027)
                     /gene="nupG"
                     /locus_tag="RHW33_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV1644489.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(44229..45308)
                     /gene="mltC"
                     /locus_tag="RHW33_000951"
     CDS             complement(44229..45308)
                     /gene="mltC"
                     /locus_tag="RHW33_000951"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV1644490.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(45373..45648)
                     /locus_tag="RHW33_000952"
     CDS             complement(45373..45648)
                     /locus_tag="RHW33_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV1644491.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(45676..46728)
                     /gene="mutY"
                     /locus_tag="RHW33_000953"
     CDS             complement(45676..46728)
                     /gene="mutY"
                     /locus_tag="RHW33_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV1644492.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLCKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFAGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            46889..47608
                     /gene="trmB"
                     /locus_tag="RHW33_000954"
     CDS             46889..47608
                     /gene="trmB"
                     /locus_tag="RHW33_000954"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV1644493.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            47608..47934
                     /locus_tag="RHW33_000955"
     CDS             47608..47934
                     /locus_tag="RHW33_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV1644494.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            48118..48837
                     /locus_tag="RHW33_000956"
     CDS             48118..48837
                     /locus_tag="RHW33_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV1644495.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            49013..50059
                     /gene="ansB"
                     /locus_tag="RHW33_000957"
     CDS             49013..50059
                     /gene="ansB"
                     /locus_tag="RHW33_000957"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV1644496.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            50176..51183
                     /locus_tag="RHW33_000958"
     CDS             50176..51183
                     /locus_tag="RHW33_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV1644497.1"
                     /translation="MKKQWIVGTALLMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSNDDLYTWVGQLADYELLEQTWTKDKAVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(51426..52562)
                     /gene="hemW"
                     /locus_tag="RHW33_000959"
     CDS             complement(51426..52562)
                     /gene="hemW"
                     /locus_tag="RHW33_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV1644498.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGSAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(52555..53148)
                     /locus_tag="RHW33_000960"
     CDS             complement(52555..53148)
                     /locus_tag="RHW33_000960"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV1644499.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDEQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(53156..53446)
                     /locus_tag="RHW33_000961"
     CDS             complement(53156..53446)
                     /locus_tag="RHW33_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV1644500.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(53443..54009)
                     /gene="yggT"
                     /locus_tag="RHW33_000962"
     CDS             complement(53443..54009)
                     /gene="yggT"
                     /locus_tag="RHW33_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV1644501.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(54027..54731)
                     /gene="yggS"
                     /locus_tag="RHW33_000963"
     CDS             complement(54027..54731)
                     /gene="yggS"
                     /locus_tag="RHW33_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV1644502.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            54749..55729
                     /locus_tag="RHW33_000964"
     CDS             54749..55729
                     /locus_tag="RHW33_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV1644503.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISMALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(55913..56329)
                     /gene="ruvX"
                     /locus_tag="RHW33_000965"
     CDS             complement(55913..56329)
                     /gene="ruvX"
                     /locus_tag="RHW33_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV1644504.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVDVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(56329..56892)
                     /locus_tag="RHW33_000966"
     CDS             complement(56329..56892)
                     /locus_tag="RHW33_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV1644505.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(57001..57951)
                     /gene="gshB"
                     /locus_tag="RHW33_000967"
     CDS             complement(57001..57951)
                     /gene="gshB"
                     /locus_tag="RHW33_000967"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV1644506.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(57964..58695)
                     /gene="rsmE"
                     /locus_tag="RHW33_000968"
     CDS             complement(57964..58695)
                     /gene="rsmE"
                     /locus_tag="RHW33_000968"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV1644507.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(58775..59482)
                     /gene="endA"
                     /locus_tag="RHW33_000969"
     CDS             complement(58775..59482)
                     /gene="endA"
                     /locus_tag="RHW33_000969"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV1644508.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTHSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(59577..60074)
                     /locus_tag="RHW33_000970"
     CDS             complement(59577..60074)
                     /locus_tag="RHW33_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV1644509.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLALANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(60151..61545)
                     /gene="galP"
                     /locus_tag="RHW33_000971"
     CDS             complement(60151..61545)
                     /gene="galP"
                     /locus_tag="RHW33_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV1644510.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT        14989 a        15444 c        16390 g        14834 t
ORIGIN      
        1 taagaaattt aaatataaga aatcgctttt agcggctatt ttgagtgcaa ccctgttagc
       61 cggttgtgat ggcggtggct ccggatcttc ctccgatacg ccgcctgtag attctggaac
      121 agggtctttg ccggaagtga aacctgatcc aacaccaaac ccggagccga cgcctgagcc
      181 aacgccggac ccagagccta cgccagaacc gacacctgat cctgaaccaa caccagaacc
      241 ggagccagaa cctgttccta cgaaaacggg ttatctgacc ctgggcggaa gccagcgggt
      301 aactggtgct acctgtaatg gtgaatccag cgatggcttt acatttaaac ctggcgagga
      361 cgttacttgc gtggcgggta acacgacaat tgccaccttc aacactcagt cagaagctgc
      421 gcgtagcctg cgtgcggttg aaaaagtgtc gtttagtctt gaggacgcgc aagaactggc
      481 ggcttccgac aataagaaaa gcaatgcggt ttcgctggta acgtccagta acagctgtcc
      541 ggcggataca gaacaggttt gcctgacgtt ctcctcggtg atagagagta aacgtttcga
      601 ctcgctgtat aagcaaatcg atctggcacc ggaagagttc aaaaagctgg tcaatgaaga
      661 ggtggaaaac aatgctgcga ccgataaagc gccatccact catacttcac cggtcgtgcc
      721 cgtcaccacg ccgggaacaa aaccggatct gaacgcttcc ttcgtgtcgg ctaacgcgga
      781 acagttttat cagtatcaac ccactgaaat cattctctct gaaggtcgac tggtcgatag
      841 ccaaggatat ggtgttgctg gcgtcaacta ctacaccaat tcaggccgtg gcgtgacagg
      901 ggaaaatggt gaattttcct ttagctgggg cgaaaccatc tcctttggta tcgatacctt
      961 tgaactgggg tcagtgcgcg gcaataagtc gaccattgca ttgactgaac tgggtgatga
     1021 agttcgcggg gcgaatattg atcagcttat tcatcgctat tcgacgaccg ggcaaaataa
     1081 tacccgtgtt gttccggacg atgtacgcaa ggtctttgcc gaatatccca acgtgatcaa
     1141 cgagattatc aatctctcgt tatccaacgg tgcgacgctg ggggaaggtg agcaagtcgt
     1201 taatctgcct aacgaattta ttgagcagtt taatacgggt caggccaaag agatcgatac
     1261 cgcgatttgt gcgaaaaccg atggttgtaa cgaggctcgc tggttctcgc tgacgacgcg
     1321 caatgttaat gacggccaga ttcagggcgt tattaacaag ctgtggggtg tggatgaaga
     1381 ttacaaatcg gtgacgaaat tccacgtctt tcatgactct accaacttct atggcagcac
     1441 cggtaatgcg cgcggtcagg cggtggtgaa tatctccaac gcggccttcc cgattctgat
     1501 ggcgcgtaat gataaaaact actggctggc gtttggcgaa aaacgcgcct gggataaaaa
     1561 cgagctggcg tacattactg aagcgccttc cattgtgcga ccagagaacg tgacacgcga
     1621 aaccgccacc ttcaacctgc cgtttatttc gctggggcaa gtgggcgatg gcaagctgat
     1681 ggttatcggt aacccacact acaacagcat cctgcgttgc ccgaacggtt acagctggaa
     1741 cgggggcgtt aataaagatg ggcagtgtac gctcaacagc gacccggatg acatgaagaa
     1801 cttcatggag aacgtgctgc gctatttgtc aaatgatcgc tggttgccgg atgcaaaatc
     1861 cagtatgacc gtgggtacta acctggagac ggtgtatttc aaaaaacatg ggcaggtgct
     1921 gggaaatagc gcaccgtttg cgttccacaa ggatttcact ggcatcacgg tcaaaccgat
     1981 gaccagctat ggcaatctga atccagatga agttcctctg ttgatcctca atggctttga
     2041 atacgtcaca caatggggta gcgatcctta ctccattcct ctgcgcgcag ataccagcaa
     2101 accgaagctg acccagcagg atgtgaccga tttgatcgcc tatatgaaca aaggtggatc
     2161 ggtgctgatc atggaaaacg tgatgagcaa tcttaaggaa gagagcgcat ctggctttgt
     2221 acgtctgctt gatgccgcag gtttgtcgat ggcgcttaac aagtcggtag taaataacga
     2281 tccgcaaggc tacccggacc gcgttcgtca acgacgttca acgccaattt gggtctatga
     2341 gcgttatccg gctgtcgatg gtaaaccacc gtataccatt gatgacacca cgaaagaagt
     2401 tatctggaaa tatcagcaag aaaacaaacc tgatgacaaa ccgaagctgg aagttgccag
     2461 ctggcaggaa gaagttgagg gtaaacaggt aactcaattc gcctttatcg atgaagccga
     2521 ccacaaaacg cctgagtcac tggctgcggc gaagaagaga attctggacg cgttcccagg
     2581 gctggaagag tgtaaggatt ctgactacca ctacgaggtc aactgcctgg aatatcgtcc
     2641 tggcacggga gttccggtta ctggtggcat gtatgttcca cagtatacgc aactaagcct
     2701 taacgccgac actgcaaaag cgatggtgca ggctgcagat ttaggcacca acattcagcg
     2761 tctgtatcag catgagctct acttccggac caatggtcgc aaaggtgagc gtctgagcag
     2821 cgtcgatctg gaacgtctgt accagaacat gtcggtctgg ctgtggaacg atacgagcta
     2881 tcgttatgaa gaaggcaaaa atgacgagct gggctttaaa acgttcaccg agttcctgaa
     2941 ctgctatacc aatgatgcct ataccgatgg cacgcagtgc tccgcagagc tgaaacaatc
     3001 gctgatcgat aacaacatga tctacggtga cggtagcagc aaagcgggca tgatgaaccc
     3061 gagctacccg ctcaactata tggaaaaacc gctgacgcgc ctgatgctgg gccgttcctg
     3121 gtgggatctg aacattaagg ttgatgtgga gaagtaccct ggagcggtat ctgtaggggg
     3181 agaagaggtt actgaaacca tcagcctgta ctcgaatccg accaaatggt ttgcgggtaa
     3241 catgcaatca accggcctgt gggctccggc tcagaaagag gtcaccatta agtccaatgc
     3301 gaacgttcct gtgaccgtca ccgtggcgct ggctgacgac ctgaccggac gtgagaagca
     3361 tgaagttgcg ctgaaccgtc cgccaagagt gactaaaacg tactctctgg acgctagcgg
     3421 tacggtgaag ttcaaggtgc cttacggtgg cctgatttat atcaagggca atagctctac
     3481 caatgaatct gccagcttca cctttactgg cgtggtaaaa gcaccgttct ataaagacgg
     3541 cgcatggaaa aacgatctga actcaccggc tccgctgggt gagctggaat cagacgcttt
     3601 cgtctatacc acaccgaaga agaaccttga ggccagcaat tacaagggcg gtctgaaaca
     3661 attcgctgag gaactggata cctttgccag ctcgatgaat gacttctatg gccgtgatgg
     3721 cgaaaacggt aagcaccgga tgtttaccta taaagcattg acgggacata aacatcgttt
     3781 cgctaacgat gtgcagatct ccatcggtga tgcgcactct ggttacccgg tgatgaacag
     3841 cagcttctcg ccgaacagca ccacgctgcc gacgacgccg ctgaacgact ggctgatctg
     3901 gcatgaagtc ggtcataacg ccgcagaaac gccgttgact gtaccgggtg ctactgaagt
     3961 tgcgaacaac gtgctggcgc tgtacatgca ggatcgttat ctcggcaaga tgaaccgtgt
     4021 cgctgacgat attaccgttg cgccggaata tctggaggag agcaacggtc aggcatgggc
     4081 gcgtggcggt gcgggtgatc gtctgctgat gtacgcgcag ctgaaggagt gggctgagaa
     4141 gaactttgat atcaagaaat ggtatccaga aggtgaattg cctaagttct acagcgagcg
     4201 tgaagggatg aaaggctgga acctgttcca gttgatgcac cgtaaagcac gcggcgatga
     4261 tgttggcaaa accaagtttg gcgaaagaaa ttactgtgct gaatccaacg gtaacgctgc
     4321 ggacacgctg atgctgtgtg catcctgggt cgctcagacg gacctttccg cattctttaa
     4381 gaaatggaat ccgggcgcga atgcttacca gttgccggga gcgacagaga tgagcttcga
     4441 gggcggtgtg agccagtcgg cttacgagac gctggcggcg cttggtctgc cgaaaccgca
     4501 gcaagggccg gaaaccatta atcaggttac cgagcataag atgtcagccg agtaagcctg
     4561 tatgccggat aaggcgctcg cgccgcatcc ggcatgaaat aaggcgcacc gggtcaacac
     4621 atatgcccga tgcgcaagct tatcgggcct acagagaggg aaagctgcgt ttatttgcgt
     4681 tggcagacac ctgtaggccg gataaggcgc tcgcgccgca tccggcatga tataaggcgt
     4741 atcgggttaa cacatatgcc cgatgcgcca tcttatcggg tcttcaggga aagccgcgat
     4801 tacttgcgtt ggcagatacc tgtaggccgg ataaggcgtt ttacgccgca tccggcaagt
     4861 tacaacaaac aacctttaac catgcttttt gatgtttttc agcaataccc tgcggcgatg
     4921 cccatcctgg caaccgtcgg aggattaatc atcggtagtt ttttgaatgt ggtgatttgg
     4981 cgttacccca tcatgctgcg ccaacaaatg gcggagtttc atggtgaaat gccgagtgcg
     5041 cagtcaaaaa taagccttgc gctaccgcgt tcgcactgcc cacattgtca gcagaccatc
     5101 cggatacgtg acaatattcc gctgctctcc tggctgatgc tcaaagggcg ctgccgtgat
     5161 tgtcaggcga aaatcagcaa gcgttatccg ctggtggagt tattgacggc actcgctttt
     5221 ttgctggcga gcctggtgtg gccggaaagt ggatgggcgc tggcggtgat gatattatcc
     5281 gcctggctga ttgccgcgag catcatcgac ctcgatcacc aatggctgcc cgatgttttt
     5341 actcagggcg tattgtggac gggactaatt gcggcatggg cgcagcagag cccgctcacg
     5401 ctacaagatg cagtcaccgg cgtcctggtg gggtttatca ctttttactc tctgcgctgg
     5461 atagccggaa tagttctgcg taaagaagca ttaggcatgg gcgatgtatt attgttcgcc
     5521 gctttaggta gttgggtggg accgttatca ctgcccaatg ttgctttaat cgcctcatgc
     5581 tgcggcctga tatatgccgt tattacaaaa agaggatcaa ccacactgcc ttttggaccg
     5641 tgtttaagtc tgggcggtat agcaacactt tatctacagg cattgtttta atgataacca
     5701 cgtcattatc aaagtgacat tttaactctt attaataacc ttagagatta tttaccatgt
     5761 cgataaaaca aatgccaggg agggtattaa tatcgctatt gttgagcgtt acaggattat
     5821 taagtggctg tgccggccat aatgaaaatg ccagtttact ggcgaaaaaa caggcgcaaa
     5881 atatcagcca aaacctgccg attaaatctg cgggatatac cttagtgctg gcacaaagta
     5941 gcggcacgac ggtaaaaatg accattatca gcgaagcggg tactcagacc acgcagacac
     6001 ctgatgcctt tttaaccagc tatcaacgac aaatgtgcgc tgacccaacg gtgaaattaa
     6061 tgctcactga gggaattaat tacagcataa cgattaatga tacacgtaca ggtaaccagt
     6121 atcagcggaa actggatcgt accacctgtg gaatagtcaa agcataacgt cgggtagata
     6181 taaattggcg cgggttgttt ttcgtgacgc acgaatttat ctcattcaat ggctgacaaa
     6241 aattcgtcac actcttaacc agagacaatc tcttaataca gacaaagagc atctgcgaaa
     6301 aattgcacgc gggatgttct ggctgatgct gcttattatt tctgcaaaag tggcgcattc
     6361 actctggcgt tatttctcct tttctgcgga atatacggtg gtttctccat cggtgaataa
     6421 accgccccgt acggatgcaa aaacgttcga taaaaatgac gtgcaattaa tcagccagca
     6481 aaactggttt ggcaaatatc agcccgtcgc cgcaccggta aaacaacccg aacctgcacc
     6541 tgtggccgaa acgcgtctta atgtggtgct gcgcgggatc gcctttggtg ccagacccgg
     6601 cgcggttatt gaagaaggtg gcaaacagca ggtctatttg cagggggaac ggcttggttc
     6661 tcacaacgcg gtgattgagg aaatcaaccg cgaccatgtg atgctgcgct atcagggaaa
     6721 aatagagcgc ctgagcctgg ttgaagagga gcgttccacc gtagccgtga ccaacaaaaa
     6781 agccatcagt gacgaagtaa agcaagctgt tgctgagcct gctgtcagtg cgccagttga
     6841 gatcccgact gccgtgcgtc aggcactggc gaaagatccg cagaaaattt ttaactatat
     6901 ccagcttacg cctgtgcgta aggagggaat tgtcggttat gcagtgaagc cgggggcaga
     6961 tcgttctctg ttcgatgcca gcggttttaa ggaaggcgat atcgccattg cactaaatca
     7021 gcaggatttc actgatccac gagcaatgat tgctctgatg cggcagttac cttcaatgga
     7081 ttccattcaa cttacggttt tacgcaaggg tgcgcgccac gatatttcca tcgcgctgcg
     7141 ctaaccgcat ttaatccagg agaatcattc atcgtgtttt ggcgtgatat tacgttgtcg
     7201 gtctggcgta agaagacaac tggcctcaaa acaaaaaagc gtttactgcc gctggtgctg
     7261 gcagcggcat tatgcagttc accggtctgg gcggaagaag ccactttcac cgctaatttt
     7321 aaagataccg acctgaaatc gttcatcgaa accgtcggcg ctaaccttaa taaaaccatc
     7381 attatggggc cgggcgtaca ggggaaagtg agtattcgca ccatgacccc gctcaatgaa
     7441 cgccagtatt accagctatt ccttaacctg ctggaagcac aggggtatgc cgtcgtaccg
     7501 atggaaaacg acgtgctgaa ggtggtgaaa tccagcgctg cgaaagtcga gccactgccg
     7561 ctggtcggtg aaggcagcga caactacgcg ggcgatgaaa tggtcaccaa agtagtgccg
     7621 gtacgtaatg tgtcggtacg cgagctggca ccgattctgc gccagatgat cgacagcgca
     7681 ggctcaggca acgttgttaa ttacgatccc tccaacgtga ttatgctcac cggacgcgcc
     7741 tccgttgtgg agcgcctgac ggaagtgatc cagcgcgtgg atcacgcggg taatcgcacc
     7801 gaagaggtga tcccgctgga taacgcctcg gcttcggaaa tcgcccgcgt gctggaaagc
     7861 ctgacgaaaa acagcggcga gaaccagcct gcgacgctga aatctcaaat tgtcgccgac
     7921 gaacgcacca acagtgtgat tgtcagtggt gacccggcca cgcgggacaa aatgcgccgc
     7981 ctgatccgtc gcctggactc agaaatggag cgcagcggca acagccaggt tttctatctc
     8041 aaatacagca aagcggaaga tctggtcgat gtgctgaagc aggtcagcgg cacgctcacg
     8101 gcggctaaag aagaggcgga aggcaccgtc ggtagcgggc gtgaggttgt ctccatcgcc
     8161 gccagcaaac acagtaatgc cctgattgtt actgcgccgc aggacatcat gcagtcgctg
     8221 caaagcgtga ttgaacaact ggatattcgc cgtgctcagg tgcacgtcga ggcgttgatc
     8281 gtggaagttg ccgaaggtag caatatcaat ttcggcgtgc agtgggcgtc gaaagatgcc
     8341 ggattaatgc agtttgctaa cggtacgcag atccctattg gtacgctggg ggcagccatt
     8401 tctcaggcga aaccgcagaa aggctcgacg gtgatcagtg aaaacggcgc taccaccatt
     8461 aatccggata ccaacggcga tctctccacg ctcgcccagc ttctttccgg ctttagcggt
     8521 acggcggttg gtgtggtgaa aggcgactgg atggcgctgg tgcaggcggt taaaaacgac
     8581 tccagctcta acgtgctctc cacgccgagc atcaccacgc tggacaacca ggaagccttc
     8641 ttcatggtgg gtcaggacgt tccggtatta accggctcta ccgttggctc caataacagc
     8701 aatcctttca atacagtaga aaggaaaaaa gtcggcatca tgctgaaagt cacgccgcag
     8761 attaacgaag gtaacgcggt acagatggtg attgagcagg aagtctcgaa ggtggaagga
     8821 cagaccagcc tcgacgtggt gtttggtgag cgcaaactga aaaccaccgt actggctaac
     8881 gatggcgagc tgatcgtgct tggcggtctg atggacgacc aggcgggaga aagtgtggcg
     8941 aaagtgccgt tactgggcga tatcccattg attggtaacc tgtttaaatc gacggcggat
     9001 aaaaaagaaa aacgtaacct gatggtgttt atccgtccga ccattctgcg tgacggtatg
     9061 gcggcagacg gcgtgtcgca gcgcaaatat aactatatgc gcgccgaaca gatctatcgc
     9121 gatgagcagg gcttaagcct gatgccgcac accgcgcagc cggtactgcc agcgcaaaac
     9181 caggccttac cgccggaagt acgcgcattc ctcaatgccg ggagaacgcg ttaatggtgc
     9241 ctgtagcaca ggaaaccacc gctaacaccg tgcgtctgcc ctacagtttc agccgtcggt
     9301 ttagcctggt ggcatggtgc gaagcgtcgc tggagatcct ccatgttcat ccgttgtcgc
     9361 tctctgtttt gcaggagcta cagcgggggc tgaacgcgcc ctttacgctg cggcaaatcg
     9421 acgaggccga atttgaacag cggctgaatg cggtctggca gcgggactct tccgaggctc
     9481 gccagctgat ggaagatctc ggttctgctg aggacttttt taccctcgcg gaagaactgc
     9541 cagaaacgga agatctgctg gaaagtgatg acgatgcgcc gatcatcaaa ctgatcaacg
     9601 ccatgctggc agaggcaatc aaagaaggcg cttcggatat ccacatcgag acgtttgaaa
     9661 agagtctggt gatccgtttt cgcgttgacg gcacattaca tgaaatgcta cgccctgggc
     9721 gcaaactggc ctcgctgctg gtatcgcgta ttaaggtgat ggcacggctg gatatcgccg
     9781 aaaagcgcgt accgcaggat ggacgtattg cgctgctgct gggcggccgg gcgattgacg
     9841 tgcgtgtctc caccatgcct tccgcctggg gtgagcgcgt ggtgctgcga ctgctggaca
     9901 aaaatcaggc ccgcctgacg ctggagcgtc tggggttgag tcagcaactg accgcgcagt
     9961 tgcgccagct gttacacaaa ccgcacggca tctttctggt gacggggccg acgggttccg
    10021 gcaaaagcac cacgctgtat gccggattgc aggagctgaa caaccactcg cgcaacattc
    10081 tcacggttga agaccccatc gaatacatga ttgaagggat aggtcagacg caggttaaca
    10141 ctcgcgtcgg catgaccttc gcccgtggcc tgcgcgcgat tttgcgtcag gacccggatg
    10201 tggtgatggt cggtgaaatc cgcgataccg aaaccgcaga aatcgctgtt caggcgtcgc
    10261 ttaccgggca tctggtgctt tccacgctgc ataccaacac ggcggtgggg gcgatcacgc
    10321 gtttgcagga tatgggcgtg gagcctttcc tgctctcttc cagtctgacg ggcgtgatgg
    10381 cgcagcgact ggtccgcacg ctgtgccccg actgccgcca gcccgcgcct gccactgacg
    10441 aagaaaaacg cctgctggga attaccgacg tgcgtaccgt cactctgtac catccgcagg
    10501 gctgccccgc ctgtaatcac aaaggtttcc gcggacgtac tgccatccat gagctgatcg
    10561 tggtagacgc cacattgcgt gatttgatcc accgtcaggc cggggaactg gagctggaac
    10621 gttatgtccg gcaacactct gcgggtatcc gcagcaacgg cattgagaaa gtgcgcgccg
    10681 gagaaacctc tctcgatgaa gttctgcggg taaccatgga ggcgtaatgg cactgtttta
    10741 ctatcaggcg ctggagcgta atggtcgcaa aaccaaaggc atgattgagg cggattccgc
    10801 acgtcatgcc cgtcagctat tgcgcggtaa agatctcatt cctgtgcata ttgaaacgcg
    10861 gatgaatgca tcggcagggg gaatgttgca gcgtcggcgg cacgcacatc gtcgcgtggc
    10921 ggcggcagat ctggcgctgt tcacgcgcca gctggcaacg ctggtacagg cagcaatgcc
    10981 gcttgaaacc tgcttacagg cggtcagtga gcaaagtgaa aaactgcatg tgaaaagcct
    11041 cggaatggcg ctgcgcagcc ggattcagga aggttacacc ctgtcggaca gcctgcgcga
    11101 acatccccgc gtctttgatt ccctgttttg ttcgatggtg gctgccggag aaaaatccgg
    11161 gcatctcgac gtggtgctta atcgcctggc ggattacacc gaacagcgac agcgcctgaa
    11221 atcacgtcta ttgcaggcca tgctctatcc gctggttctg ctggtggtgg caacgggcgt
    11281 ggtcactatt ttgctgacgg cagtggtgcc gaaaatcatc gaacagttcg atcacctcgg
    11341 acacgcgctg cccgccacca cccgcgcgct tatcgccatg agtgacacgt tacaggccag
    11401 cggcgtttac tggctggcgg gattgctggg gcttctggtg ttggggcaac ggctactcaa
    11461 aaatcctgcg atgcgcctgc gctgggataa aaccttgctg cgcctgcctg tgacggggcg
    11521 tgttgcgcgc gggctgaata cggcgcggtt ttcccgcaca ttaagcatcc tcaccgccag
    11581 cagtgttccg ctactggaag gcattcagac cgctgccgcc gtgtcggcaa atcgctatgt
    11641 cgaacaacaa ctgctgctgg cggcagatcg cgtccgcgaa ggaagcagtc tgcgcgctgc
    11701 actggcggag ttgcgcctgt tcccaccgat gatgctgtac atgatcgcct ccggcgaaca
    11761 gagcggcgaa ctggaaacca tgcttgagca ggcagcggtc aaccaggaac gggaatttga
    11821 tacccaggtg gggctggcgt tagggctgtt tgaacccgcg ctagtggtga tgatggcggg
    11881 cgtggtgctg tttatcgtca tcgccatcct cgagccgatg ctgcaactga acaatatggt
    11941 tggaatgtaa tttacggagt tatcacatga attcgttatc ccgcacacaa aaaccacggg
    12001 caggttttac cctgctggaa gtgatggtgg tgattgttat tcttggcgtt ctggcaagtc
    12061 tggtggtgcc taacctgttg ggcaataaag agaaagcgga tcggcaaaaa gccatcagcg
    12121 atatcgtggc gctggagaat gcgctggata tgtaccgact ggataacggg cgttatccga
    12181 ccacagaaca ggggcttgag gcgctgatcc agcaaccggc caatatggcg gattcccgca
    12241 actaccgtac tggtggatac attaaacggc taccaaagga tccgtggggc aatgattatc
    12301 agtatctcag cccgggtgaa aaaggagtgt ttgatgttta caccctgggg gcggatggtc
    12361 aggaaaacgg ggaaggcgct ggcgcagata tcggtaactg gaatttgcag gagtttcagt
    12421 aatcagtgcc tgaacgcgga ttcactcttc tggaaatcat gctggtgatt ttccttatcg
    12481 gccttgccag tgcgggcatg gtacagacgt ttgcgaccga ttctgaatcg cccgcgaaaa
    12541 aagcggcgca ggattttctg actcgctttg cgcagtttaa agacagggca gtgatcgaag
    12601 ggcagacact cggtgtacta atcgacccgc cgggatacca gtttatgcaa cgtcgtcagg
    12661 ggcagtggtt gcccgtttct gcgacccggt tatcggcaca ggttacggtg ccgaaacagg
    12721 tgcagatgct gttacaaccc ggaagtgata tctggcagaa ggaatatgcg ctggagctgc
    12781 aacgtcgtcg tctgacgctg cacgatattg aactggagtt gcaaaaagag gcgaaaaaga
    12841 agacgccgca gatccgtttt tcgccttttg aacccgccac accgtgtacg ctgcgcttct
    12901 actcagcggc gcaaaacgca tgttgggcgg taaaactggc acacgatggc gcgttatccc
    12961 tcagtcaatg tgatgagagg atgccatgaa gcgcggattt accttgctgg aagtgatgct
    13021 cgcactggcg atttttgcgc tggctgccac ggcggtgtta cagattgcca gcggtgcgct
    13081 gagtaatcag cacgttcttg aggagaaaac ggtagcaggc tgggtggctg aaaaccagac
    13141 cgcgctgctc tacctgatga cccgtgaaca acgggcggtc aggcatcagg gcgagagcga
    13201 tatggctgga agccgctggt actggcgaac cacaccgcag aatacaggca acgcgctgct
    13261 ccaggcggtg gatattgaag tcagccttca cgaagacttt tcgtcggtaa ttcagtcacg
    13321 acgcgcctgg tttagcgccg tgggaggcca acagtgagaa gggctcgcgc tggtttcacg
    13381 ttactggaaa tgctggtggc aattgccatt tttgcctcgc tggcactgat ggcgcagcag
    13441 gtgactaacg gcgtcacgcg cgtgaatagc gccgtcaccg gacacgatca aaaactaaac
    13501 ctcatgcagc aaacgatgag ttttctgacc cacgatctga cccaaatgat gccgcgtccg
    13561 gtaagaggcg atcagggcca gcgagaacca gcgttactgg ctggcgctgg cgtgctggca
    13621 tctgagagtg aagggatgcg ctttgtgcgt ggcggcgtgg ttaacccgtt gatgcgtctg
    13681 ccacgcagta atctgctcac cgtcggttac cgcattcatg acggctatct cgaacggtta
    13741 gcctggccgc tgaccgatgc cgcaggcagc gtgaagccga caatgcaaaa gttgattccg
    13801 gcggattcgc tccgcttgca gttctatgat ggtacgcgct ggcaggagag ctggtcgtca
    13861 gtgcaggcga tccctgtggc agtgcgcatg accctgcatt cgccgcaatg gggcgagatt
    13921 gaacgcatct ggttgttacg cgggccgcaa ttatcatgat cacctcacca ccaaaacgcg
    13981 gaatggcact ggttgtggtg ctggtattac tggcagttat gatgctggtg actatcacgc
    14041 tttccgggcg gatgcagcaa caacttgggc gaacccgcag ccagcaggag taccagcagg
    14101 cgctgtggta tagcgccagt gcagaaagtc tggcgttgag cgcactcagt ctgagcctga
    14161 aaaatgaaaa gcgcgtgcat ctggcacagc cgtgggcttc cggccctcgt tttttcccac
    14221 tgccgcaggg gcaaatcgcc gtcactctgc gtgacgcaca ggcgtgcttt aacctgaatg
    14281 tcctcgctca gccgacgacg gcgtcgcgtc cgctcgcgtt acaacaactg attgccctga
    14341 tctcgcgcct cgatgtgcct gcttatcggg ccgaactgat agccgaaagc ctgtgggagt
    14401 ttattgatga agaccgcagc gtgcagacgc gtctgggccg tgaagacagc gaatatctcg
    14461 cccgctcggt gcctttctac gccgccaatc aaccgctggc cgatatcagc gagatgcgcg
    14521 tggtgcaggg aatggacgcc gggctttatc aaaaactgaa accgctggtc tgtgcgctgc
    14581 cgatgacccg ccagcaaatc aacatcaata ctttagacgt cacgcaaagt gtgattcttg
    14641 aggcgctgtt tgacccgtgg ttaagccctg ttcaggcgcg ggcgttatta caacaacgtc
    14701 cggcgaaggg ctgggaagat gtcgatcagt ttcttgcaca gccgctactt gctgacgtcg
    14761 atgagcgtac taaaaaacag ctaaaaaccg tcctgagcgt ggacagcaat tacttctggc
    14821 tgcgttcaga tatcaccgtg aatgagattg aactgacgat gaactcgtta attgtccgca
    14881 tgggcccaca acacttttct gttctctggc atcagacagg agaaagtgag tgagttccat
    14941 ccttgagatt tttttcccgc tttgcgcctc cgacaccatc cgctggcagc gccgtacacc
    15001 cgacgtggaa cacggtatct ggcctgacgt tgctgacgaa cgtctccagc aatggctgca
    15061 aactgatgcg attcgactct acattcccgg cgaatggatc agcgtctggc aggttgaact
    15121 gcctgatgtc gcccgcaagc agataccgac tattctgccc gccttgctgg aagaagagct
    15181 gaaccaggat atcgatgaac tgcatttcgc cccgttgaaa atcgaccagc aactggcaac
    15241 cgtggcagtg attcaccaac agcatatgcg caacattgcg cagtggttgc aggcaaacgg
    15301 catcacctgc gctaccgtcg cgccagactg gatgtctatt ccttgtgggt ttatggctgg
    15361 cgatgcgcaa cgggttatct gccgcatcga tgaatgtcgg ggatggagcg ccgggcgggt
    15421 gctggctccg gtcatgttcc gtgcacagct caatgagcag gatttaccgc tttcgctaac
    15481 cgtagtcggc attgcaccgg aagagctatc tgcatgggct ggcgcagacg ctgaacgcct
    15541 gaccgttacg gtcctgccag ccgttaccac ttatggcgaa ccggaaggga acctgctaac
    15601 agggccgtgg caaccccgcg tcagctaccg aaaacagtgg gcgcgctggc gggtgatgat
    15661 tctgccgata ttgctgattc tggttgcgtt ggcagtggaa cggggcgtga cgttatggag
    15721 tgtcagcgaa caggtggcgc aaagccacgc ccaggctgag aaacagttct taacgttgtt
    15781 cccggagcag aagcggattg tgaatttacg ctctcaggtg acgatggcgc tgaaaaaata
    15841 tcgcccacag actgatgata ccgatctgct tgccgagtta tcagcgatag ccagcaccct
    15901 gaaaacggcg tcactttccg acatcgagat gcgtggtttc acctttgatc aaaaacgcca
    15961 gacgctccac ctccagctac gggccgcgaa ctttgccagc ttcgacaaac tgcgtaccgc
    16021 gctggcggca gattttattg tgcaacagga cgcgttacag aaagagggcg atgcggtttc
    16081 cggcggcgta acgttgcgga ggaaataaca tgttacgcga taaatttatt cactattttc
    16141 agcaatggcg tgaacgccag ttaagccgta gcgaacactg gctggcacaa cacctggcgg
    16201 ggcgttctcc gcgtgaaaag ggcatgttac tggcagcggt ggtgttcctg tttagcgtcg
    16261 gatattacgt cctcatctgg cagccgttga gcgaacggat agaacaacag gagacgatgt
    16321 tgcagcagct ggtggcaatg aacacccgac tgaagaacgc cgcgccggat attattgcag
    16381 cgcgaaaatc tgccacaaca acgcctgcgc aggtatcgcg ggtcatcagt gacagtgctt
    16441 ccgcgcactc ggtggtcatc aagcggatag ccgagcgtgg ggagaatatt caggtctgga
    16501 tagaacctgt ggtgtttaat gacctgctga aatggttaaa agcactggat gaaaaatatg
    16561 cgctgcgggt gacacaaatt gatgtcagtg ctgctgagaa gcttgggatg gtgaatgtgc
    16621 agcggctgga gtttggacgg gggtaaagta tcaggagatt aatatcaata aaattactct
    16681 cgttaacgtg gcgagagtaa ttcacccaga ctttataagc taattttttt cgtgaaggtt
    16741 attgtcagat ccaaaagcaa aaacccgcct tgtgggcggg ttcttaagaa ttagtggtgc
    16801 ccggactcgg aatcatatcc acgattaaca tactgtttgt tattggtttt gttgcgattt
    16861 cttgtaaaca gcgcccccac taaagccccc ataaaaaatc gctttggttt tgtgagggtt
    16921 atcacgaaaa atcaatgtta agtggagggc ttcgttatgt gtggagttat gaccctggcc
    16981 caaaatcagt gcagtgctaa atcagagatc ttcgtctttc cggagacttc ggatgaattc
    17041 agccggagtc atgtcattta atgatgaatg cgtcctttca tgattgtatt ccctgcgcca
    17101 gctgtcgagt ttgtcctgcg catcttccag tgacaggaac cagtgaatgt tcagacactc
    17161 atcccgtaga ctgccgttaa atgattcaat aaacgggtta tctgtcggct tcccggggcg
    17221 cgagaagtcc atggtcactc cgtgttcata cgcccattta tccagacttt ttgagataaa
    17281 ctcgctgccg ttatctgtct gaatacgtac cggcaggcgc ttacccggca cccgcaaccc
    17341 ttccatcaca ccgaccacat cctcgccttt cagcgacttt ccggcatgga tcgccagaca
    17401 ctcccgacta aaattatcga ctacagtcag cgcccggaaa cgccgtccgt taaacagatt
    17461 atccgacacg aaatccatac tccagcactg atcgacaccg gtcaggatgg gacgctgttg
    17521 gcgatgtgct gcactgacat gcctgcacgg acgttttctc cgcaggttaa gtccttccag
    17581 acaataaatc cggtgtgttt tcttgtggtt aacaggccag ccttcctgcc gtaactggat
    17641 atgaatacgc gggcaaccgt agcggatccg ggtttccgct atctccctga tgcgcagggt
    17701 tatcgcccga tcatctcgcc gactctgcca gtggtaaacg gttctgctct gtatcagcag
    17761 tccacatccc cgtcggacac tgatacggta agcttcccgc agaaaatgta ccgcctgacg
    17821 cttctgagcc ggcctcagaa cttttgtttc agtacctcct gcaacatctc cttatccaga
    17881 ctcagatcag ccaccagttt cttcagccgc tgattttcat cctccagttg ccgcagacgc
    17941 cgcagttccg tcacgcccag accggcaaat ttcttcttcc agttgtaaaa ggtggcctca
    18001 gaaataccca tctttctgca gacttccccg acgcgggtgc cggtttcggc ctgtttcagc
    18061 gcaaacgcaa tctgttcttc ggtataacgg gtctttttca tggtaataag cctctctgtg
    18121 agcaggaaga aagaccggag acttcagttt agactggtac tgttttcagg ggggagatca
    18181 gttaggttca cacataacga attctaaaaa caccgcccta aattttccgc tagttctcta
    18241 ccatcaaccg ggcaagcttg gcaaaaatcg aacacttgct gctttcaccg ctcctgagca
    18301 tatccagggt aaagctattg agataaacgc cagttaacgg gtggctaaat acatctacgg
    18361 ctttgccttg ggcatcccat atcagcagat tttccaccag gttaggccgt gcaatagtag
    18421 gatttgtagg ctgggcatcg cttaccagaa caaatacacc attctcatgc ggttgcagat
    18481 aaatatggtg atgcttcatc ttttgcgctt tggtcagcga gtcgtatttc gagcggccag
    18541 gtgatatcag atagggcagt acgttttgat ccggttttgc agagcgggtg atggcgtacc
    18601 cgttaaattt atccccttta cgctctgcca gagttttttc ttgttcctga taagcgtatg
    18661 aatcgacgat attaaattgc gccgggccaa tcaggcgcgg tacgcgggag ctgttgtagg
    18721 tggtaagcag gacatctgct gtgtggtcgt agagatagaa gcttttgtcg aataatttct
    18781 ttaatcgtcc cagagagggg ggatcaacag acagatagtc taatttacct tcagcgatcc
    18841 cggtatcaac caccctaagt tggccgtgtt ctatttttag ttcgacatat ccaagcaggg
    18901 ggagatagct tttcgttttg cgtactgcat aggcggtata gtgcccgtcc gccaaatctg
    18961 gtagtgggat gtgatgctga ttgagtaagc tctctttgaa ccatagctcg gtttttgttc
    19021 ggcgaacctt actgttaagt aaggtaactg ctgacttgag acgactgtga ggcttcgtaa
    19081 gatcgatttc gtattgagta gggttgcttg tcaattgttc attaataaaa tcaatcgcat
    19141 ccaacaactt gtgatcaata ttcataccct gtaagactac gggcagaggt tgctgattta
    19201 gccacccttg caaacgatct agtttaagct gggtataagt atcccaattg agttcgggtt
    19261 gttttcgggc gtcggcaaag gccgcataga aatcattgta ctcaacagat tcattatctt
    19321 gctggcggat gctgttgctc cgctcttcat ctactgcatt gagtaccaga atagatgtac
    19381 tggcaggcag ctcagaaaag ccggtttgct gagaatgttt aagcgacgcc agaggccaga
    19441 gttgataccc ggggaactgg atggctaatg tgtcaaagaa ataagcttct tgcgtggcgc
    19501 taaattcgac gccgttatta accaccaaca gcgtattagt cagttgttga tcgaggtcgg
    19561 tagcgaactg attgacctcg tgggtggcct gaaatgcgat aggctgatgc gggatctcta
    19621 gttcactgag ccgctcgcag gcggcattca tgaccacgtt ataggtatag gcctggcatt
    19681 caccatagcc gctgcgctca cgaaaaaagt ccagtttgct atcccgggca tcgagtttgc
    19741 gccgggcttt gagccgacga ttatcgatgt taaaccacac gcaggtatcg tccagagaga
    19801 gatgcaattc atcctccggt tcgaccagaa attttcgttt gtgcagggta aaggcgagtt
    19861 cgttatattc tgatgcataa atgtcgagtt cgaatgcctc catcactcca gtgccgcttt
    19921 tctgcgtgac gggctgactc aggcgagcgt tgagatagaa cgtagagttg aagtaggttg
    19981 gcggtaccga aaagcgctcg ctggcttgtt ccagcattac cattttcaaa tgcagactga
    20041 acaatccctg aatcaggcga gataaatcat aattttcagc tgtgagtcgc tggcaaagcg
    20101 tttcaatgcg ttctgccaaa gtccgatgcg ctaggctgtt cggtgcagca gcaggcgcca
    20161 ggaaaaaaaa gacgtgatgg taattgggta agcaatgcag ctcatatttt acttcctgtc
    20221 cggcctcaag cccaagcttc tgccgcacgc aagtttttac ctcctgatga attcgcgcat
    20281 agtaatcagc ggatttttct tttcgctctt tagtaaagtg ctgaaccata aagtctatcg
    20341 tatagatatt agataccggg gccaatagac gctggattgt tgtcagctga tcttcttcaa
    20401 gcttgatcaa attggtacgg gcaatcggag tatccgtcag ctcattcaga ttagggatca
    20461 tgatactttt cctttagtcg gcaacttttc tacacccagc agggttagta acattgggtt
    20521 tatatccaat gtcatgcgca gtttcgagtt ttcctgagcg cgtccgctct ctcgattttt
    20581 gggctgataa tactggtagt cagtttgcaa caggttgagg tagtggatcg tgtgatccac
    20641 attatcctgc cacatcagat tattagggtt ctggctgtag gcgacgttca gatagataaa
    20701 tcgaatacgt tcataacggc ctgccagcgc cgtctggagc tgtttctgcc cctcagtatc
    20761 cgccttttgg ctggcgatat tacggatctg gcgaatcttg ttgtcgcttt tctcaagcgt
    20821 ttcttctgcc cgcgtcaaat cgtccaactg tgtcgcccag cgcttaacgt cgatgcagag
    20881 caggtcgtcg cccacttcaa taaagcggtc aaaaaactca tagaccagcg gttcaaggcg
    20941 ttcaaacacc tgctggtcgg agagtggggt aacaccataa ctttttagca ctttatcgaa
    21001 gagatattca ccgacattgc ctttgagcaa cggaactagc ctggggttgg gtacccattt
    21061 tttgaatgcc ttttcctgga tgttgtcaca ttcacgaatc aactcgccga ccaggttgcc
    21121 gggggtaaaa tcgacatcgt ttctatactg cggaaagagg gtgagctctg gccggtactc
    21181 tcttgcgccg ccagcgaaat ccgacagggc ggaataatct cggtgggcaa gtccatccgg
    21241 gccgcgtttg tggcaaagta aaatcgtttg attcccttgc tgacgatcga taaacagagc
    21301 gtcgtgaaca gattgcattt gtcgattggc ggtatacaag ggattagcct ggagttttgc
    21361 cagccagcgc tggggatcgg taaaggagtc aggatcgcgg aataaattac atatctcgag
    21421 ataatcgaga ttgccagcgc gtaccttatt aatccagtcg gttttaagga cacgtttgtg
    21481 aatttcatcg atgcggcgac cattcgcgac gatagcttgc tcaaacgtca gtcgctgttc
    21541 cgcattattg aatgactggc cctgactcag cttttcgcac tcctccatga gtcggtggtt
    21601 aagcaaagac atactttctg ataccacctc gttaccgcta tcttcactaa gagcggcgaa
    21661 ctgaaatgca acattacgga acacatcgcg gggtaaaaag atttctgttt tcaatagagt
    21721 gtcgcgacgc tcgactcgcc ctacggcctg cacgacgact ttgaataagc tcatatcatg
    21781 ttcggccatt aacaggcgat agttttcgcc gtgggcgaaa ttaacgttga aatcggccag
    21841 cttgtggacg gtaatatcgt catcggcgta gtgtttaagc acggtaacgt agttaggtaa
    21901 tgtattgagg ttgccgctgt tgtcctttac ttcgctgtaa aacgaggagt tgatgagcac
    21961 taagcgctca aaatcgacat ccagacgtgg tgcattgata tcattaatat cgccgtcatg
    22021 gtatttaaca aagtagttga ggccggtacc cgcactttta taactgctca taaataccag
    22081 tacggtattg ctgttctgga gataggtttc ttgcctgata tcttcgacat tagccagtgg
    22141 tgaatcgaaa aagaccaaat ggacggtgtg acgccctttg aatggggtaa aggtcaggat
    22201 ctggtcatgt ttcttaccgt tatccgtttt ttcatcgcac cgggagtgca taccgtactg
    22261 ctgacgccag gttgtctggt gcgtgcgcca ggcgctgata aaggcccgct taaacgtccc
    22321 tgaaagtgac agaatcaggc tgtttttacc ctcccaggcg gcaagcagta acgcttccag
    22381 ttcccgctgg cactgacgcc gtttataggt atttttcagg tcgtattcca gcggtttctt
    22441 cagcgcgtcg aaaaagttgt catacgtcct gcgatagatt tcacaatttt ggtagatatc
    22501 ggtcaatttt aactgcttat catcgaacac cctgaagtct acgttgcgga tactggccct
    22561 caacccgcgt aatgccttaa gcgtatcgat atctgccttt tcgcgttcaa cgacccggta
    22621 gccaaggtcg cggctatagt gcgccaggaa gtgacgattg aagttaccgt ttttggtgtg
    22681 gctaaaaccg ctggtggcgc taagcccaat tactttattg tgcgtgccgg ttaataaacg
    22741 cagcagcatg gcttcaggca actcttgtac cagatccatc tcgaacgcca gaataattgt
    22801 gcgctcggct cccctgttca cgtaattgag ttcagctatc ggcgtcatcg tgaatacggt
    22861 tttgggttgc aggtaagtgt agaaatgatc aatcaacaag tttttatcgg tggtgcgatc
    22921 gaagatatgt cgcaccacgc cggctacgtt attggctgcg tcaacaaact ggcccaaagg
    22981 cgtattctgg ctgctggagt tgtcctggcc accattcttt acccagcgct taaagtgccg
    23041 gttggtgatt tcagaacagg cggcgaggat gacggagacc aactggaaca gatcgtgcag
    23101 agtggggttg gtgtcattgg catcattttc gacttcgtaa tacagttcgg tgcgcgttat
    23161 gtcgccttcg ctgttgcgca tgcgaatgcg tttcagccct tcttcattga cgtacatttt
    23221 ggggttaaag ctgaatacgt tgcgggtgat tgagatgatg cgttcggcgg cgtcgccgtt
    23281 gacttcaaat gcccctaact ggtcacgaaa catctccagg atcgatccca gccttgtacc
    23341 gggggatagt tcgcactttt ccgccagtaa attgcgcaga gtagtgatga atttgaccat
    23401 ctcttgctca aaggtcgttt gcgcttcttt gggcttgttt cggcgttcta agctgagtag
    23461 cgcgttgtga atacgtcctg cgacggaaat aacgtgcgcc aggttatttt cctgtgtgat
    23521 tagctttaca tggcatgttt cttcaaggcg agtgtaggct tcatgtagtt catcaatgat
    23581 caccgtaaaa cgaatatttt tctggcgaaa aggacaatct ggattgcgtc tgaagtgttc
    23641 gtcgcgaaga taggtaacct gcccggtatg accggctgcc gcaacggtgc taatctgtgg
    23701 atctttggga gtcagcttac cgccaatcag caagtcgaaa cctacggact caaaacgcac
    23761 accttcgcct cgctgacgag gcgccagtcg gtaagttgat gtgtcgaact tattcgtggt
    23821 cattaatagc actgacgggc ggtactggca aacttcgaaa ggcaatacct gtttgataag
    23881 ctcgaaatag acttcgtgta cgcttatctt aggttcaagc tttcctggtt ttcgtgctgt
    23941 ctccgcgttt tgcattcgct cttgccgcgc ctggagcccg cgacgaatgt actctttaat
    24001 ggaagctttt tcactatctg gtccaaatag taatttacag gctgactcaa tcgtattgcg
    24061 gatactgtga cggcagtttt ttagctgttc ttcgagaatt tctctttcgt agttggtatc
    24121 ctgagaaccg tatgttgtca gcttttttaa ctgctcttca cagcgatcaa tttgtaagac
    24181 atgataattg agcgaacgca tagcgccgcc gatatatttg ctgcctttcg ccccttcgta
    24241 ccactgaata tagcggtcgc ggtttttcag accagaggcc cagtccataa agtcgaggtc
    24301 ggcaacatcc ttacgggaga gaacgcaaat gaactcgccg ctagcggcca gaattttctc
    24361 tttctgactg ctgtccaggt cgatttgtga tttttgcggt gacatgaata gcaggttggt
    24421 aaagccacct tcaggaaaca gatcgtctga cttttgggtt ttggcgaagc gctccagata
    24481 ttcgacatac ccttggatca cgccatagct tttgccgaac cccgtgccgt cggaactgaa
    24541 gatgacctca gcgccatcac gttcatgcac gtggtaaagt tgagagactt gggcgcgaca
    24601 ggcgttaatc tctttaatca gcgttatgtc aggaatttgc ctagagatga gatcttgcag
    24661 attattattg atatttttct ctccaacacg ctcacacttg cggtaactat tgatggttat
    24721 ttggccgctg tcgatcagaa tcttttccac ctgcatgggg gcaagatgca aggctgtcgc
    24781 gatttcattg atagtattgg acagtggttc gtgtggattg ctaatcgtga agtggtttag
    24841 tgctgccgca acggtatctg gatgcaatgc ttttctgagg gtgggaaagg tgaggctgta
    24901 atcgtatagc gccaaatcat tgcttatcag gcagcgaaac agttgtttag tgggatcgcc
    24961 accaggacgg ctggcaaatc ccaagatttt tgcctgttcg aggcgataaa gcaatgctgc
    25021 ggcatcgcgg tcaggtgagt caaatgcatc gtggagctgc tggctaaatg gcattgtggc
    25081 attcagccgg ttatggctga ccagcgcgga aaagagctga cgcaccaagg ttagcagctt
    25141 acaatcttcc tctatagtcc agctttttct cattcttctt ccccttaata taaatgcccc
    25201 tgcaaccact ccgtcacgcg ttcaatatta aaagcacgta atccatattc ctgcgtcagc
    25261 aacgtctcgg gtaaatactc atcatatttt tcgaggcact tttcaacgat gctttcggca
    25321 agacgggatt cgtaaggtag cccttcctga agtgcgctga ataacgcttg tttaacctcc
    25381 gagaccgtag ttttttctac ttcaaccaca ccagcaaatg agcccgcctt gaaaccgcgt
    25441 tggataagta atgccgacaa cgtgtttacg gtttgatcgc ctagccaggg aagaatgtag
    25501 acatgctgtc tctgctgaat aaaacattcc gaagccagat tattgttgcg aaaacagtgc
    25561 agcccttcat caaacaggtt tttggccgtg gtatcggcaa aatcggcctt gcgattgcca
    25621 acggtgatgc ggtagtcgcc ttcccgataa atagtgagca tttcttggcg gacgacatca
    25681 tgaatggaca tcccttgtcc gccaaataac ggcggctgcc caccctttgt cgcctcgaca
    25741 taaataactt ttttatcact atcgatatcg gttaccttcc agcgtcgacc gccgaaaata
    25801 atgtgttgat caggcatcag tggggaatca acgggaatgg agcccagtgt tttgctcccc
    25861 gccacaatgc gaaactcttc cggcgtgctg aacacggcgt agaaggtgta ttgatttacc
    25921 tgacgttcgc cctcgacgcc cagaaccagt tcgccgcttg agagctgggt cagaaactgg
    25981 tgctccccca tatgtttcaa taacgttttg aagtcataga tccggacttt ctgaaatggc
    26041 ccttgcaggc atagctgtga ccagatctga tccgcacgca cgcctcccca ctgcgccacg
    26101 atcgccagga tctgatggaa cagggtggaa tagtgcatct gccgggtatc agctggctca
    26161 aaccatttgt tgccgataag taagcggatc atggccagcg actgaacaag ctgaagcctg
    26221 agctggtcga caatgcctga tgttggcgtc agttcatgtt cggcaatcag cattctcaat
    26281 acggcaggcg agtcgcgccg accggagcgt cccattcgct gacgcaggct ggctacggaa
    26341 tggggggcgg taacttgcac aacggagctg actttaccga tgtcgatgcc aagctctaac
    26401 gtcatcgtac agatggcggt ggtgggtaag ttgccttgtt gaagcctctg ttccagcgtt
    26461 tcacgcagat ctctggacag agatccgtga tggggaaaga actcattggg aacgatgctc
    26521 gcttcactga gatcgctaag cgtggcggca atgctttcgg tccgtttgcg actattagcg
    26581 aacaccagat gggaatcacc acgacagagg cgaaagatat catggcagat ttgcgtctct
    26641 gccgacggtg gagattgctg gcccgaggtg gtcagcggtt ccagataacc tttcacctgt
    26701 acttttagcg tggcgtgagt ctgactgtcg gtaataatgt cacagggcag acgttgattt
    26761 ggccgcagag ataacggcac ctgttccagt tcccccagcg ttgcgctgag tgcgactcgg
    26821 gggactggat tgttgattct tcccagcagg tgatcgactc gatttaacag agagagaagc
    26881 tgcatacccc gctcagaacc gatgaaagca tggaattcat caatggcgat atatgccagt
    26941 ggcgcgaaag cctgctttaa ccatcccgca ttgcggatca gcatcgcttc cagcgattcc
    27001 ggggtgataa gcaaaatacc ggcaggattc ttctttgctt tcagcttttt gctctgcgca
    27061 acatcaccat gccagggcgt gaccggcatc tccaacgcat caccgaggtt ttccagcctt
    27121 cgatactgat cgttaatcag ggccttaagc gggctgatgt ataaaatgcc aaagccgccc
    27181 tgaatatccg caatggcaga acaggcgggc aggaaaaacg cttctgtttt acctgcggca
    27241 gtcgcggcgc tgatcagaac atcccgatcg cccgctaata tcggtgaaac ggattttttt
    27301 tgcagttccc tgagatcggc ccatccctgc cggtatatcc acttctgtat ccgtggatcc
    27361 agccgctggt attcgttcat cataatctga agtcggccag accgtcctca tcggcatctt
    27421 ctgccgatgt tatttcatcc atatcactgg gtttttcttc cgcgatcgcg acaccggcga
    27481 ttaactgtga ccactgaatg gatgggtttt gttccagcac ggccagcata tccaggaagg
    27541 ctttaatcgt gtttcgtggc gtacggaaat aggcatcgcc aatagtgttg ctacagtggc
    27601 gtaaaaatgc cgtcagagca tcatcaggaa ccagatactt atccgcatcg ccgccggaat
    27661 aaacgtgacg aaggtttttc aacagaatat agagttcttc cggcgtcagg ctggctaagt
    27721 gcagggaagg ggacgaataa tcaatgacac cagcccgctg agcgaagcta ttttccgcca
    27781 gtcgggactg gagcgcttcg tagctgtaca accccttgcg cggatcgaac aggaattctg
    27841 gcgtaccgcc aagtaaaaaa ccgatatatt cagccgtccc ttgcaggcag tcattcagaa
    27901 tacgcagtat ctgttcatag ttggcaacgc gggcctgagt gttactgagc ttatacagat
    27961 tgaccatctc atccagattc accagcagac ccgcgtatcc ggcctgacgg acaaacaggc
    28021 tcatcagctt tagcgaatcg tagaaagagg catcagaaat aatggtgcgc acacccagat
    28081 cgttatgggc gtcggttttc gtggtgtatt cacctcttag ccagcggatg gcatttgatt
    28141 tcaatgtctc attatcctgc tcgtggccct gccagtaaca ttcaatgact ttggcgaaat
    28201 cgtaaccgcc aaccatatca gacagggcgg cgagcttttg gtgaataatc gtcggaactg
    28261 acacatttgt actttctgct tcttttctgg cttccgtgat aaagcgctca accacgctta
    28321 ataatgcgtt tccatccggc ttatttcggg tggatagatt tttcattagc tcggagtaga
    28381 ggttacgcgc ctgcccaccc gtcgcgtgga tgcgcctgtc cggggataaa tcggcgctga
    28441 ttgtcaccag ctttttttct agcgcaattg agcgcacaac gcttaaaaag aatgtcttac
    28501 ctgagccata ttccccaata atcagccgga atcctgcgcc gccatcagcg atacgctcaa
    28561 tatcctgata cagcgctttt atttcgttga cccggccaac ctgaatgtgc tgaataccaa
    28621 ttttaggcgt aacacctgac ttcagtgact gaataatagc gtctctttct ttgagacgaa
    28681 tacgaatgcc agacataaat tatcctttga gttcttgtgc aatttccagg tcaacgtaaa
    28741 tatcatcgtc atcatcaagc accggggcgt caacctgttc gaaagaccag tcattaattg
    28801 cttcaatcgc gccgcttagc atcaaattaa actgctggca tagctcagcg acttcgtttc
    28861 gcgcccagcg ttctttttcc tgcaaacgtt ggtaaagttg atgatgtgct tcatcaagac
    28921 ctgcgccagc gtgtggcggg atctccgctg gggattcgtc tgcgggttca tcttcggtga
    28981 agatggtgtt cagtaactgg cgtacgtccg ttgtgtcgga ttcatgacgg gcaaggatcc
    29041 gttcatcaag agaaaacgcg ccgctcgtcg ctgatggggt ttgtaacgta gctgtcggag
    29101 ttgtttctgc ggttgacagt cggtggatat cgctggtaac ggcactgctg tccagaccga
    29161 ggctggcgta gattttttcc agttgtttga tttcagccgg atcgattttt ccatctgcgc
    29221 aggcgacgct gataatcacg ttgccaatag tggatttatc tttatcgctg agttgctcaa
    29281 ttttaccttt cagaccagcc tgatttgcag gcgtattgag ccgccaggtg aggtaggcgt
    29341 gcagcgaacg tttttctgtt ggcgacagcg catcgttatg attaatcgtt ttctccagcg
    29401 cggcctgttc agcaacatcc acgcgcttgt ccatttgtgc aatcatggca cccaaccgaa
    29461 gcgccacaga gactgacgta aattccgccg acggtacgta gaattccgca tgcccttcag
    29521 gaaataaaac aagatgacct tctggatccg gtctgacaag gtgatagcgc atatccggcg
    29581 caatgccgta gcctgctcgc cgggcgaaat tttgcatcag ctcggcttct ttcttattaa
    29641 tcttatccgg tacaggcata cccagtctgg cccagaaatc agccactgtc gccagtcctg
    29701 aatgttcacg gattttctca tctgcccagt gtttaaattc agcaaataga cgttctgcat
    29761 cttcggtcag tatttcatcg ggaagcagca tgatggcggc gacatcactt ggtgaggcat
    29821 cttttttacc gagatagcga ctgtaggcat ccagcgcgtt gatacaggat tctgcaacag
    29881 aaattaattt ctgaactggg gcttttaaaa cgctgggatc gggaagatcc atctgttttt
    29941 ttacctgaag ttcccgaagc gtaccactgg cgggggtata gcttaaatac aaccgtgttt
    30001 tattgggttt gacgacaata ccgtcaccgt atttttgagt ataacgctgt ttgaataatg
    30061 cggaaaattc agcatgacat cgacgggcag gcgtgcgcag ggtgtaatca ggaaaatagt
    30121 atatccatgc cagtgccaga gcggcacaaa taggttgtcc tttatcgacc acagtcgcta
    30181 gatttaatct gaacagtaat gaactcctcg atgagaaata ttcgttttcg gtatatatag
    30241 aaagcaactt cggtcggaga acgatcatca tttccagcaa ctgcgttgca taatgccgga
    30301 aggaaccgct tgcctgaaat acggttctca ggcgcgatat ctcttcgaat aatgctttga
    30361 attcatcgtc agaaatggct tcttgtgtgc catcggccag tacgcggcgt tctagaccgt
    30421 aaaaatagat aaaaacatag ccaacggggc agctcgcatc gctgcgatcg cttgccagcc
    30481 agtcaagata ggcgccacgg cagcgagggg agagtgtggc aaagctgggc cagtatccca
    30541 gtgactcatc ataataatgc cgtgaaccag gctcgatggg aaaagcgtca ttaaccagcg
    30601 aagcgtcgga gtcatcgtta taaagatatc catattctcc tgatgaatgt gtttttaacc
    30661 gcccgccgaa ataaaaataa ccgtgattaa tgacgacatt ttgaatggtg atgctttctc
    30721 ccggtttgac ccatcgcgct ggagtggtat tgctacgggc ggcgttttct cgtgacggct
    30781 ggcggctggt gctgtattca accgtctgcc cgttcacaaa agtaaaggtt gccagttcgt
    30841 catcatcgga ggcctgagaa tgcatatttg tcatgctgtt atctggctta ttgagagatt
    30901 tctgtcgaga tgaatgattt attttggtga tgattttgtt gtttactctg gggggcggcg
    30961 attttttctt tttggaaaac agaagccaga taaccgccag cataataaca aatatccaga
    31021 agcccattcc ttgcccttaa tcgctgataa cgcttcccgg cattgttcta cgataggcaa
    31081 aggcggtaag cataaaactg gaagagagat cgtaaaatga taccgcgaat gggctagagt
    31141 acaagcataa aaaccctatt attcaaaagg attatgtcat atggctgggg gagcaattgg
    31201 caggaccagg ttatacctct gaatcacgca taaaacgatt gatttcatct tgcgcatgac
    31261 gaagacgatc ggcaatgtca gcaaatctgt caggctgatg aaagaacatt cccccttcca
    31321 ccgccgtttg atgatccccg gcgatcgctg cgaaccgttc agcatcgtca ggcaacagtt
    31381 gcaggtttcc gacaatcacc tgagtaaaat ccacacccgg cacagaccag cgacgttgct
    31441 tcatctcgac cacattgcgc atggcttcca gcgtttcata ggggctggca aaatgattac
    31501 tcagcagaca atccacatca taccaatggc gtgccagtcg ggccggattt ggcgctttgg
    31561 tttgcgtgca gaactggtgc agcgccgtca gcttttccca aagaatataa ccggtgtcat
    31621 aagcgtttac ggtcgcggtg ggtaactgga gtgtatccat gcctgcgatc ccggacagat
    31681 agcttacaac aggcatcaga tcggttggct tgccacgatt tcgtccgcca aattccagca
    31741 gaatatgatc gcgctgatag gccatactgt cttctttcac ccgtgggaaa tagatatcga
    31801 ctttttcgcc accactgtcg ggttccattt ttgcttcaag gcccggaatt tcataagcct
    31861 gaaagcgttg gtttaaccgt tctgccagcg cgtttgtcca ttctgttaag cgctcttgct
    31921 gctgctctct gaaccggcga ttctggctgt tatttagcgt gctttgctcc catgcctgct
    31981 gttcttcctc ttctgagtgg cctgccagat cggcccagtg aacagaaaga tcgatatctt
    32041 cagaaaaacg ctcaatcacg ttccagcatt tgcttaacgc cgttccacct ttaaacgcga
    32101 cagataaacc tcccagcagc tcctcattaa aaagtaaccg aaggatctcc gttacccaga
    32161 gatccttttc cagaaagctg gctcccagcc cggagggatg ctgctgcgcc gcgtaagaga
    32221 ggatttcctg aagctcgcga cgttgtgatg cgctccggta gtaatcaaaa atgtccatca
    32281 gagcgccttt tgcaacgtta tcagctgcga gcgccacgcg tgaagcgcag gctcattcat
    32341 caactttccg gcaatctgcc gtgctttagc ggacgggatg ctcagccgat gaaaagcatt
    32401 cttcagcgtt tccaatgggg tatgttgctg atccagactc agcaaaccgc gcatcagctc
    32461 tccttcaggc cggttcagcc aacgtaaccg acttgctgat ttatgctgga tccgtacccg
    32521 ctcattgccg acgcggaatt ctcgcgtagg gccgtttgac cagtagatgc gtttaactgg
    32581 cgcctgcgtt tgcagcccca gccggtaagc ggcttccagc ccctgaaggg tgattttgta
    32641 accacgctct tttgcccatg cggcggcaac ggcattggcg ctggcaacaa cgttaacaga
    32701 ggggatgttt ttcaacggct ttggccggga gtagaaaccg cgttcaaccc ggaccagcag
    32761 gccttctttg accagacggc tcattgcctg ttgtaccgac gcacggcttc ccagcgagta
    32821 aaacccgctg atggaaaagg ggatgccacg cttcatgcgg tgaacccgct ggcgggccag
    32881 agcggcaaca gacattgatt ctcctgtact gacgttttca ttgatatgta ttttcattat
    32941 atcccttgtg tagcaaggag tctaacctaa aaaatgtcag taatattcat tctatgacaa
    33001 tgtttaaaat ggcagatatc agtgaggtag cttgctgtag gaaattccga cgataaaata
    33061 cccacataca gcaggcggtg ataaggatat agcatgagca ttctctccat ccacggcgtt
    33121 cgcagttatc agggcgaaag gccagtggag ttcgacttga gtaagtatgt cactcttatc
    33181 tatggacaaa acggctcagg aaaatcaacc gtttctggtt acttttataa gcatggtcgt
    33241 ccagattaca gccagtgcag cctgcgacct ccactggata tgaactacct ggtgtttaat
    33301 caggagtatg tggacgatat tttcagccaa ccttctcagc cgggcatttt cactttaaat
    33361 agtgaaaatg cagaaattaa aactgaaata gatgctctgg aagcggagag taaagttttg
    33421 tttgcccggc gtgatgcttt ggacgtgcaa aaacgcgacg ttgagggcat ggaagaaagt
    33481 attaaaaatg gctccgcaaa acagatttat agttctactg cagaaatcag aaaaacagat
    33541 ttatgggatt tgatgtctgg aactaaacag accgacaaac tttttcagac catccttgaa
    33601 cacactgaag tcgaagatac ttcgacgcag gaattaaccg aagaactaca ccgacttgaa
    33661 gccagtaaag gcaaccctta cgctttgctc gaagcattgc cagcatcccc tttgaatgac
    33721 aatgatattg cgctattgat gcaacctctt attccagcag gtgatagccg tcttgctgcc
    33781 gcaattaacc aattgggcaa cattgactgg gtgaggaatg gacagcagtg gctgagtgat
    33841 gacatttgcc cattttgtca aacgccgata gatgcccggc aacttcaaca ggaaatcact
    33901 gccctttttg atacctcatg ggaagctgcg atggaccagt tgcgggaact tcaggcgcga
    33961 tatcaattct ggcacgataa acccgaacat atgcggcagt taataaaaac gtgtccgtta
    34021 gttgatcagg aacatcctgt ttatctctat ctacttgaac tggagcaggc gtatcaaaga
    34081 aataaattgc atatcgatga gaaattgaca tcaccctctg cgtcgattgc tgttgaggat
    34141 ctttctgctc tggctggcaa tgtctctgtg cagatagcca gtattaatac aataataagc
    34201 gaacataacc gaaaggctga aaattatcag actgaacggg ttagactgaa acagagacta
    34261 ctcagtcata tccgaaaact ggctaccgat acgataatta accatgatga acaactagca
    34321 gaacttgctg aaaaattggc aaaattaacg gtgtctcggg atgaaattac ggcccagctt
    34381 gatacgctga acgcaattat tcgcggtaaa tccagcctga tcgtcaatac gcaggaaacg
    34441 attgaacgta ttaatcacag cttggatagc cttggaatca ctggattccg cattgctccc
    34501 tatgacgatc gggatgatta tcgtctggtg agggaaggtg agaacagcga tacgcctgtt
    34561 tttagtagtc tgagtgaagg ggaaaagacg ctgattgctt ttctctattt tctggagact
    34621 tgcacaggga gaaaaagtcg tgatgacaat gaccaacgaa aacgtttaat tgtcattgat
    34681 gatcctatat ccagcctgtc gcaagactat gtttttgaaa tagcatcgtt gatccagcat
    34741 caggttatac gtgccagaat tggtgagaaa gtgattattc tgacgcacag cctgtttttc
    34801 tttcaggagt tactgctttc tgcggaacgg aagaaaaggg cagcgggttc ttgcccacca
    34861 gaatggacgc tttatcgggt atccaaatcc ttacatagtt ctgcttccct tgtttccgaa
    34921 aaagaattac tgaacgacta tcaggcactc tggtatgtct tacgccacgc ccaaaaagat
    34981 gacattgcca gtgtggtgat cccaaatacg atgcggcaaa tccttgaata ctattttggt
    35041 ttttcgggga aaagtgagac gcttcaccgg gcgcttgaaa cactggcgag cgggcctgaa
    35101 ggtgaacctg gtttccgtat atttgctcgt tatctaaatc gacattctca ccaggatgcc
    35161 cgcaatatct ctcttcatga gggggcttcg gttgaacgct atcttacctg gtttaagaaa
    35221 gtattcgagg cggcaaagga tgaagagcat tacacatcaa tgatggaaaa gacgacacaa
    35281 acgacctgat taacaacaga agaggaccat caccccggga ttaaccgtta gcagttcggc
    35341 aaagagcgca tgtgctggca cacgtgctcc cagcgaccaa aacggaaacg cagatactgg
    35401 tgaaccagca cagttttctg tttcataccg ggctcctttc tttcgacgcc gacaagctgt
    35461 cggcgagccc cgtacacctc gtacgagcgt gactatcgtg tagaggctaa ggatcccggg
    35521 ggatggcttc cgctatcgta ggcgatttca tttgatgatt caatctttgc tttggataac
    35581 aattaattga gatggtcgta ttttagtatt agcactgtat gtcttagcta tagtgctgct
    35641 gaaatgacca attcgggaac cgtgatggtt tcctggaaac tctcttgctt attttacgat
    35701 gctggccgat tatcacaaaa cgttgcttaa acgctgattg gatatggtgc tttgtggcag
    35761 tcattcgctt gtctgacgtt gccatggctt acatttgatg tagccaatgt agaagccgct
    35821 tggtgaattg ttctatgaca tggtctacta cttcgttgtg gaaaatgaag atgcggtacc
    35881 gggtatgtac cattgatggt gagaggctta tcgacgaatg gaccgtacga tattgcttgg
    35941 ctggaaaggt gtgtgaagca ttgtagattt acattcacaa aatcgcgaga aaatctacaa
    36001 acaactctat ttccagaaaa atgagtttcc cctttctgcg ccagaaataa aggcgtaatc
    36061 tccctcctgc actatgttgt cgtggacaac gcccttgctt catggtctgc aagaagcccc
    36121 tgatttatca ggatgaccag ccctttctac cggtacgacc tccgcaatac cggaacgcga
    36181 aagcgtttga ggctaagagt acatacacat ggacggctcc atcgtcatcg cttaacggca
    36241 acagccttca ccggctgaac ggcctctctt tgccggaatt ttcctggccc acttataccc
    36301 tgctgataac cagcgccttt ttacatgacg ggctgcggcc tgcattgccg aaggctttgc
    36361 tgccgcgtga ccgtcatcct gcaccagagt gttaattttc agaccctgcg ccagaccagg
    36421 gcgtcagtcg ctcacgcgca gtgagagact gacgcccgca gaaatttcca tctgcaccag
    36481 tcatatcaac tgttccagcc acaggcgagc ttctgacgtc gcttttcagc atttatctca
    36541 accattcatt cacaaaagga gaacatgagc cagtaccgga gggggaccac caggacggtc
    36601 cgggggatga ccagatcacg ctggcggctg ctctgagagc acaaccgata ccccgcaaaa
    36661 cctcaacagc cgtcgccccg gcgctcaggt actgctcgca gccccgacaa aaatcagggg
    36721 ctatcggcct acgcgccggg gaaagctgta tccgttgttt gttgtggttt tgtgaaaatc
    36781 aggagcgaga gaagaggaaa acgtaaaatg ggtatgctgg aaagagagat gaaaaacctg
    36841 gcgcggcagg ctggcgggtc gcacaagact gtccatgacc gtatcgccat ggcaggacgt
    36901 ttctgcgaac gcctgatgga actgaacgtt cagatacgct atgtccacca tttaaaggcg
    36961 aagcatattg aggcgtatat tcagatgcgg ctggcgcagg ggattcagaa aaatacgctg
    37021 cataacgaaa cggcggctat ccgtaaaatc ctgacgcagg ccgggcgcga aaagctggcg
    37081 cagagtgaac ggatatcgaa taaatcgctg gggctggctg gcgtatcccg caatggtacg
    37141 cggaaagcca tcacgccgga atattatcaa cagatggcag aggctgcccg gctgaaagat
    37201 gcggggctgg cggcggcgct ggaactgacg cgactgatgg ggctacgctc tcaggaggca
    37261 gtacgctgtt ccggttcgct gaaaacctgg cgcgaagcac tggagaaagg tgatacccgg
    37321 ctaaccgtgg tactcggtac caaaggtgga cggccccgtg acacgattat tcaggatact
    37381 ggcgcagtta aacaggcgct ggataaggcc atcgtggtgg cagaacggcg caacggcagg
    37441 ctgattgatg cggcgagcct taaacaggca atgaaatact ggcgcaacca gactttgcgt
    37501 atggggctga cgggcaaata ctccccgcac tcactgcgct acgcctgggc gcaggacgat
    37561 atccgccggt atctggcgca ggggttcagc gaaaaagaag cgctggcgat ggtggcaatg
    37621 gacctagggc atggcgacgg gcgtggtcgc tgggtgaagc aggtgtatgc acggaatgat
    37681 gaagcgtgat tttttgctgg cgcagcgaaa ggaaaaaggc caaagggtaa aggtgggtta
    37741 agggtaacag ctccttaccg gaaagggcca aagggttggg actggcgcag atcgacgtct
    37801 atccacggat atctggcgca aataaaaagc agatccctga tgcagtggat ctgctttccg
    37861 gcattgtccg ttagctggcg caggccgcct gaatctgcct gtcataccga tacggcggga
    37921 taaatttctg ccgacaggcg tcgagataat ccgaccacca ctgcatcatc tgtgtgcgct
    37981 cgtcgaggtg ctccgccttg tggatatacg cggcacgaac gtgattacgc tcctgatggc
    38041 tcatttgtcg ctccacggcg tcttttgacc agcgcccgga ctcgatcagc gcactacagg
    38101 ccatcgtgcg gaaaccgtga ccgcaaacat ctgcctgtgt gttgtagccc attacccgca
    38161 gtgctttgtt gatggtgttt tcgctcatcg gcttacgcgg atcgtggtcg ccgggaaaca
    38221 gtaactcact actgccggac aacagtttta actgtttgag tattgtcacc gcctgcgctg
    38281 acaacggcac cagatgctgc atcttcatct ttgcgccgcg atttgaataa cgcacgcccg
    38341 gaatcggctc ccgctccgcc gggatggtcc acattgcgcc atccagattg atctccgtcc
    38401 agcgggcaaa acgtagctcg ctggaacgga taaagatcag cagattaagc tgtaccgcca
    38461 gacgggttaa cagcctgccg gaatagcctt caatacgctc cagcatctcc ggtattttct
    38521 ccagcggcag ggcggggtag tggtttttgg gcggcggtgc gatagccccg gtcagatcgt
    38581 tcgccggatt gctgtcgatt atcccctcct gcaccgcgta acgcatgatt gccgtggtgc
    38641 gctgctgaat ccgtgcagcg gtctccagcg taccttttcg ttccgccacc cgcagcggga
    38701 tcagcagatc ggcagttttc aaatcagaga ccggaatttg ccgatcagtg gaaaaacatg
    38761 cagctccagc gagcgcagga ctttggcggc atgggcttca ttccagcgga tattgcccgc
    38821 tacccactta cgggcgatat cttcaaaggt gttgccgctt ttacgtgcaa acttctcctg
    38881 ctgttttttc acgccgggat cgctgcctgc gctcaatacc gagcgggcat cctcgcgcag
    38941 cttgcgggca tttgccagag aaacggccgg gtaagcacca aacgccagct tcttctcttt
    39001 accgccgaag cggtacttga ggtgccagag tttagatccg ttgggcttta tcagcaggta
    39061 cagaccctgc gcatcgctga gtttccagga tttatcaagc ggctttgcat tacggatggc
    39121 ggtatcggtg agggacatag tggggctcca tagtttatcg aaccgacggg ccccaattaa
    39181 gcccccaaaa actatggatg tcaaaaaatc tggcagtacc tgttggtact ccagactctt
    39241 tgtaactaac tgattttttg atgtttcagg actcatagag acgccatgaa actaaatagt
    39301 ggtgcccgga ctcggaatcg aaccaaggac acggggattt tcaatcccct gctctaccga
    39361 ctgagctatc cgggcaacgg ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat
    39421 ttcttcaatt tactgcagac tgcacaatct atcatcactt tgccgctatt gcacgcattg
    39481 tcaggcaaac caggcggtcc aggcggcgga aagcggcatt gccagaagga gtgctggaag
    39541 catgttgacc accgggaaca ttttaatgcc acagatgcgt aatccggttg ccagcagcaa
    39601 taaaccgcct acggcgctga agtctgccat catcgacggt gtggtcagcg gtaatatcag
    39661 cgcggcagcc cacgccagcg ttaactggat gatcagtaat gggatactaa ttaccgacac
    39721 cgcaataccc agcgagcagg cgaagatcat cgccgtaaag aaatcaagaa atgacttggc
    39781 gattaaaata ctcggatcgc cggtcatccc ttcgttcatc gccccgaaga tcccggtgcc
    39841 gctggcgcaa aacaggacaa taatcgcgac aaaattctga atgaaagatt catgcgctgg
    39901 cttcttacgt gagtgacgaa acagattttg tgctttggcg accgctgtat tgacgccttt
    39961 ctccagcaga caaatttcgc cgattaatgc tccgagtaag gttgctaaaa ccatcgccgg
    40021 aaggttggcg catttcacca ccagtaaaat accaatcccc agcgatgcca gaccaaaaat
    40081 tgatgtcatg gagacgcgga tacgttccgg taagcgttgg ctaagcagtg caccgagcaa
    40141 gccacccagt aatactgcac ttgcgttgat aaaaggaccg atgaccacaa gagttcctgt
    40201 tagctgcatt gtctgatttt cttattatga ccgtttggtg ccgtcgatgg cttgtttgtt
    40261 cgcaaagtcc tggcttgcac gctttagcga aaggtgccat gattgcgcga attttctcct
    40321 ctctgtacgg agtttgcccg atgcacgcca tctccttaca ttctctcgct tatcgccgtt
    40381 tcgcgcgaaa cgtttccctt tctatgttac tgctcatgcg gtgaagttaa cgcacgctca
    40441 ctgcaggaca acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc
    40501 tggtgaccag tttgacccat atctcatggg gcagggtttt ccaccttgtc cggtattctt
    40561 acttccccga aacgggtttg cgcttatgaa atcaatgaat attgccgcca gtagtgaact
    40621 ggtatcccga ctttcttctc atcgtcgcgt ggtggcgttg ggagatactg attttacgga
    40681 cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt ggcattctta cgttgcttaa
    40741 gcgcaccggt tttcatctac cggtgttttt gtattccgaa catgctgttg aattacctgc
    40801 gggcgttacg gcggtaatca acggcaacga gcagcagtgg ctggagctgg aatccgcagc
    40861 ctgtcagtat gaagagaatt tgctgccacc gttttatgac acgctgacgc agtacgttga
    40921 gatgggcaac agcacctttg cttgccctgg acatcaacat ggtgcgtttt ttaaaaagca
    40981 tcctgccgga cgccattttt acgatttctt tggtgagaac gtctttcgcg ccgatatgtg
    41041 taacgctgac gtaaaattgg gcgatctgct tattcatgaa ggatcggcga aagatgcgca
    41101 gaaattcgca gccaaagtct ttcatgccga taaaacctat tttgtgctga acggcacatc
    41161 ggcagcgaat aaagtggtga cgaatgcgct gttaacgcgt ggcgatctgg tgctcttcga
    41221 ccgtaacaac cataagtcga atcatcacgg cgcgctgatt caggcggggg cgacgccggt
    41281 ctatctagaa gcttcacgca acccgtttgg tttcattggc ggtattgatg cgcactgttt
    41341 taatgaagag tatctgcgcc agcaaattcg cgacgttgcg ccagaaaaag ctgagttgcc
    41401 gcgcccgttt cgccttgcga ttattcagct gggaacctat gacggcactg tctataacgc
    41461 ccgtcaggtg atcgatacca ttgggcatct gtgtgattac attctgtttg attccgcgtg
    41521 ggtcggttat gaactattta tcccgatgat ggcggatagc tcgccgctgc tgttagaact
    41581 taacgaaaac gatccgggga tttttgtgac tcagtcggtg cacaaacagc aggcgggatt
    41641 ctcacagacg tcgcagatcc ataaaaaaga taaccatatc cgcggacagg cgcgtttttg
    41701 cccgcataag cggttgaata acgcctttat gctccatgct tctaccagcc cgttctaccc
    41761 gctgtttgcc gcgctggacg ttaacgccaa aattcatgaa ggggagagtg ggcgtcggct
    41821 gtgggctgag tgcgttgagt tgggtattga gtcgcgcaag gctattcttg cgcgctgtaa
    41881 gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa ttgtggcagg attatccgac
    41941 atcagtgtta gccagcgacc gccgtttttt cagttttgag ccgggggcga agtggcacgg
    42001 ctttgaagga tatgccgcgg atcagtattt tgttgatccg tgcaagctgt tactcactac
    42061 accaggtatc gatgccgaaa ccggcgaata tagcgacttt ggcgttccgg cgacgattct
    42121 ggcgcactat ctgcgtgaga acggcattgt gccggagaag tgcgatctca actccattct
    42181 gttcttatta actccggcgg aaagccacga gaagctggca caactggtgg cgatgctggc
    42241 gcaatttgaa cagcatattg aggatgactc gccgctggct gaggtgttgc cgagcgttta
    42301 taacaaatat ccggtgcgct atcgcgacta caccctgcgc cagttgtgtc aggagatgca
    42361 cgatctgtat gtcagtttcg acgtcaaaga cctacaaaaa gcgatgttcc gccagcagag
    42421 tttcccgtca gtggtgatga acccccagga tgcgcatagc gcttatattc gcggtgacgt
    42481 ggagttggtg cggattcgtg atgccgaagg gcgaattgcg gcagaagggg cgttgcctta
    42541 tccacctggc gtgctttgcg tggtacccgg ggaagtctgg ggtggggcgg ttcaacgtta
    42601 tttccttgca ctggaagaag gggtgaattt gttgccggga ttttcgccgg agctgcaagg
    42661 tgtttatagc gaaaccgatg cggatggcat gaaacggttg tacggttatg tgttgaagta
    42721 agaataaaaa aaacgggtca ccttctggcg acccgttttt ctttgcgtaa ttagtggcta
    42781 accgtctgtg tacctgtcgg gacacgaacg tgtttatatt tgaacatcgc catgaacgcg
    42841 aaggccagaa ccacggagta accagcgaaa atcaaccata cggtctgcca gtcggtaatg
    42901 ccgttttggg tgtacatctc aaccacttta ccgctcacga tgcccccgag gatacagccg
    42961 aagccgttgg tcatcatcag gaacatccct tgcgcactgg cgcgaatagc cgggctaact
    43021 tctttttcga caaacaccga accagagatg ttgaagaagt cgaacgcaca gccgtaaaca
    43081 atcatcgaca gaaccagcag tacggtgccg aacggagtcg ggtcgccgta agcaaacagc
    43141 gcaaaacgca ggatccacgc cacgatactg atcatcatta cgttcttaat gccgtagcgg
    43201 cttaagaaga acgggatggt cagaatgaac agggtttcag agatctgcga aatcgacatg
    43261 atgattgacg catgctgcac aataaagctg ctggcaaaca tcggatcttt gtcgaagctg
    43321 tgcaggaagg tattaccgaa catgttggta atctgcagtt ccgcgcccag cagcattgag
    43381 aagatgaaga agattgccat acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc
    43441 agggttgtcc agctctgatt cgcttgctgt ttagcaaccg gaatatgcgg cagagtcagg
    43501 gtaaacagaa ccagaatggc ggaaagtgct gcgccaatat acagctgcat gtggcttaat
    43561 tcgaagccag acaggctcac cacccacatt gccatgataa agccgatggt gccccagata
    43621 cggattggcg ggaagtcagt aacgatatcc atcccggcat tttgcaggcg atagtaagag
    43681 atggtgttga ttaacccaag cgttggcata taagcaaacg agttaatcaa tatcacaagg
    43741 aacatcgctt ccggcgtcgt gacctgtgcc gccatgaaca gcgtgatagc gccaatggtg
    43801 tggcaaatgg catataccca tttcgcactt aaccatttgt cggccacaat ccccagcagc
    43861 gcaggcataa agaccgcggc gatacccagt gaactataaa ctgcgccaat agaagcaccg
    43921 tcaaacttca gggtaacaaa catataggag ccgagggtcg tcagccaact tccccacaga
    43981 cagaactgca gaaaagagag gattttcagc tgcagcttaa gattcatgtt aatttcctca
    44041 catcgtgatg cggatgagtg tttgaaaaag cacatttgac cgtgcgagat gatgggattc
    44101 tatcaacttc gcggactggt tttttgttac ctgtggcaaa taattgcaaa cattcctaat
    44161 ttgcaaaacg aaattgtgat atggataaca tttttgcccc tgagcatcgt caggggcggt
    44221 tagtggaatt atcggcggcg gtaggatttt tgcgcggtat tcactttata aagataacgg
    44281 cgagattccg cagagggatg gcgggtcgtc agcgtttgat aaacatcgcc cggcgtcatg
    44341 gtgttaataa tattagccgc ctgaatctta tcattcgaaa agactcgcag cacgctgcct
    44401 gcgccgccgt tataggctgt gatgacggca taacgccggg atgtagggtt atcaattccg
    44461 ccgagataaa cattgttcag catcgccaga tacgcggtgc cggtatcaat attgctggca
    44521 ggatcaaaca agaaactgcg gctcggcgtg ccggatttcc cctgcgagcg gaacacatct
    44581 ttcccggcag tatgttgtac cacctgcatt aaccccagcg catcggaacg gctgaccgca
    44641 tacgggttaa aggaagattc agtctgcata attgccagaa tcagcgactc atcaacgcca
    44701 tatttccgtg acgcctggcg gaccatgccg agatatttgt gcgcacgttt atcaaggtgg
    44761 ttcggcacca tgttaatggt gacgctgtag atgatacgca gcccgttgct gcggctcttc
    44821 agacggtttt tcagcagata atccgcgaag ttgcttgcgc gaccttccca gcgaatcggc
    44881 tgcccggtgt tgtccaccac ctgaccgtaa aggaaaggtt ctttcgaaat tgtaatatca
    44941 tcaacgtcgg aatagagatc gaccgaactc ggatcgtcac ccatcagcaa cgttttgata
    45001 attgcccggc gcaaatgcgc ggcaggttct gtcccggcga tggtttcgat agtaatcgta
    45061 ccgtcatcga agttgatgtg gctgcgggtc tgatattgat cggtgtattt cacgtagtcc
    45121 ttaggaccgg cgatcaccac ctctttgaat ccccagatgt tctcaatatt gtgggcaaat
    45181 tgccccatca gaatatcaaa accgttggtg tctttgaccc aggcttcgtt ataggtatcg
    45241 ccttttttgg tcgtcgaaca ggagatgagc aacggcgcaa tcaaagccag cgcgagatat
    45301 tttttcatca ttccgggtgc gtgttgtgtt tgtttttatg caagctgccg gaggcgtgct
    45361 ccggcactgt ttttattttt tatcttccgg cgtatagccc tcgatatgca cctctttacc
    45421 ctcgaacagg aagttgacca tctcctgctc cagcagcttg cggtgctcgg cattcatcat
    45481 gttgagtttc ttttcattaa tcagcatggt ttgtttgtgc tgccactgcg cccaggcttc
    45541 tttggagatc tcgttataga tgcgttttcc cagctcgccg gggtacagct gaaaatcctg
    45601 accttctgct tcacgttgca ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct
    45661 ttatcgactc acgcgctaaa ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac
    45721 gggagccgcc aggccaactg acggcggttg cgctaagtta taccagagcg cattgccttc
    45781 atccatgcag ccggcgaatg acgacacggg aagccacata ggcacaatat ctaagtggaa
    45841 atggctgaag gtatgccgaa acgcggtcag ctgcgtcagg ttatcggcag caatctgccg
    45901 ttgcgccagc cactgccgca aactttcttc gtcggcaaac tgcgggaaac agtataaacc
    45961 gccccacaat ccgctcggcg gacgctgcgc cagcaatact tcatcttcgt gctgtaacag
    46021 gagaaagtag ccggtgcgct ccggcagcgt ctgtttcggt tttttgcccg gataaagcga
    46081 ccagctattg ttggtggcgg caatacatcc gttttgtagc ggacagagcg aacatttcgg
    46141 cttcgaacgc gtgcaaatca tcgcgcccaa atccatcatc gcctgattaa accgttccac
    46201 gccgaccgcg ggcgtcacct gctcgcttaa actccataat ttattctcga cctctttttt
    46261 cccaggccag ccgcttacag catagcagcg cgccagcacc cgtttgacgt taccgtcgag
    46321 aatcggaaag tgcttacaca gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac
    46381 gcccggcagt gctgcaactt cctcaaaggt ttccgggaat ttaccgccgt gtaaggtcgc
    46441 cacttgttgt gccgctttat gcagattgcg cgcgcgggcg taatagccaa gcccggtcca
    46501 caagtggaga acttcgtcga gcggcgcatt ggcgagatcg gtcaccgtcg ggaagcgcgc
    46561 cataaagcgt tcaaaatagg ggataacggt cgcaacctga gtttgttgca acatcacttc
    46621 tgagagccat actttgtagg gcgtcttgtc aatttgccag ggcagagttt ttcgcccgta
    46681 tttatcgtac cagtccagaa cctgggctga aaattgcgac gcttgcatgg tcaccgaatt
    46741 cactgttgtt gggggcaaga ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc
    46801 gcagccacgg ccttatcatg cttgcatccg gcaactaact ttggataatg cccgttttca
    46861 gaacactttc acaagcgact aaatctttat gaaaaacgac gtcatttcac cggaatttga
    46921 tgaaaacggc cgcccactgc gccgtatccg tagttttgtg cgccgccagg ggcgactgac
    46981 caaaggccag gaacatgcgc tggaaaacta ctggccggtg atgggcgttg agttcagcga
    47041 agatatgctg gatttccccg cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg
    47101 ttttggcatg ggggcgtcgc tggtggcaat ggctaaagat cgccccgagc aggacttcct
    47161 cggcattgaa gtgcattcac cgggcgttgg tgcgtgcctg gcttctgcgc atgaagaggg
    47221 cttaagcaac ctgcgcgtga tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc
    47281 tgacaattca ttgcgcatgg tgcagctctt tttccctgac ccgtggcaca aagcgcgcca
    47341 taataaacgc cgtatcgttc aggtgccgtt tgccgaactg gtaaaaagca aactgcagct
    47401 ggggggcgta ttccatatgg cgaccgactg ggaaccttat gcggaacata tgcttgaagt
    47461 gatgtcttct attgacggtt ataaaaacct gtcagagagc aatgattacg taccgcgtcc
    47521 ggcatcacgt ccggtgacga aatttgaaca acgtggtcat cgtcttggtc acggagtatg
    47581 ggacttaatg ttcgagaggg tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa
    47641 aaaatgcaca tcgacgaatt ccaggaatta ggattttcgg tggcatggcg attcccggaa
    47701 ggtacatcgg aagaacagat tgataaaacc gttgatgatt ttattaacga ggttatcgaa
    47761 ccgaacaaac tggcctttga cggcagcggt tatctggcct gggaaggtct gatctgcatg
    47821 caggaaatcg gcaaatgcac tgaagaacat caggcgattg tgcgtaagtg gctggaagag
    47881 cgcaaactgg atgaggtacg caccagcgaa cttttcgacg tttggtggga ctaagaaagc
    47941 atacgggcga tgacaaatgc aaaactgcct gatgcgctac gcttatcatg cctacaaaga
    48001 tgcacgatcg agtaggcggg ataaggcgtt tacgccgcat ccggcatgga aaacgcgtac
    48061 tttgttatca atctggggcc agcaaatgct ggcctgattt gttcttgagg gaagactatg
    48121 atgcgcaaaa tgctgctggc ggcagcactt tcagtgacgg caatgaccgc tcacgccgac
    48181 taccagtgca gcgtcacgcc gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg
    48241 aagggcgaaa acggcaatct ggtgatcacg ccagacggca acgtgatgta taacggtaag
    48301 caatattccc tgaatgccgc ccagcgcgag caggcgaagg attatcaggc tgaactacgt
    48361 agcacgctgc cgtggattga tgaaggcgcg aaaagccgcg tcgagaaagc ccgtattgct
    48421 ctggataaaa ttatcgttca ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc
    48481 aaacttgatg cgcagctgaa agagcagatg aaccgcatta tcgaaacgcg cagcgatggc
    48541 ctgacgttcc actataaagc cattgatcag gttcgcgctg aaggccagca attagtgaat
    48601 caggcaatgg gcggaatttt acaggacagc attaatgaaa tgggcgcgaa agcggtgctg
    48661 aaaagcggcg gtaacccatt acagaatgtg ctgggaagcc tgggggggct gcaatcctca
    48721 atccaaaccg agtggaaaaa gcaggaaaaa gatttccagc agtttggcaa agatgtttgt
    48781 agccgcgttg tgactctgga agatagccgc aaagccctgg tcgggaattt aaaataatcc
    48841 tctattttaa gacggcataa tactttttta tgccgtttaa ttcttcgttt tgttacctgc
    48901 ctctaacttt gtagatctcc aaaatatatt cacgttgtaa attgtttaac gtcaaatttc
    48961 ccatacagag ctaagggata atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt
    49021 tttcaaaaag acggcacttg ccgcactggt tatgggtttt agtggtgcag cattggcatt
    49081 acccaatatc accattttag caaccggcgg gaccattgcc ggtggtggtg actccgcaac
    49141 caaatctaac tacacagcgg gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca
    49201 actgaaggac attgcgaacg ttaaaggcga gcaggtagtg aatatcggct cccaggacat
    49261 gaacgatgat gtctggctga cactggcgaa aaaaattaac accgactgcg ataaaaccga
    49321 cggcttcgtc attactcacg gtaccgacac gatggaagaa accgcttact tcctcgacct
    49381 gacggtgaaa tgcgacaaac cggtggtgat ggtcggcgca atgcgtccgt ccacgtctat
    49441 gagcgcagac ggtccattca acctgtataa cgcggtagtg actgcagctg ataaagcctc
    49501 cgctaatcgt ggcgtgctgg tggtgatgaa cgacaccgta ctggacggtc gcgatgtcac
    49561 caaaaccaac accaccgacg tagcgacctt caagtctgtt aactacggtc ctctgggtta
    49621 cattcacaac ggtaagattg actaccaacg taccccggca cgtaagcaca ccagcgacac
    49681 gccgttcgat gtctctaagc tgaatgaact gccgaaagtc ggcattgttt ataactacgc
    49741 taacgcatcc gatcttccgg ctaaagcact ggtagatgcg ggctatgatg gcatcgttag
    49801 cgctggtgtg ggtaacggca acctgtataa aaccgtattt gacactctgg cgaccgccgc
    49861 gaaaaacggc actgcagtag tgcgttcttc ccgcgtaccg acgggcgcta ccactcagga
    49921 tgccgaagtg gatgatgcga aatacggctt cgtcgcctct ggcacgctga acccgcaaaa
    49981 agcgcgcgtt ctgctgcaac tggctctgac gcaaaccaaa gatccgcagc agatccagca
    50041 gatcttcaat cagtactaat cacctcgccc cggtatcgtg ccggggcttt ttcacttcag
    50101 acttacgtcc attgccaatt tttattacca tgatgataat cactggaata aattattccg
    50161 cacgagggtt ttcgggtgaa aaagcaatgg attgttggca cggcgctgct tatgttgatg
    50221 actggtaatg tctgggcaga tggcgaaccg ccaactgaaa atatcttaaa agatcaattc
    50281 aaaaagcagt atcacggcat tctcaagctt gatgctatca ccttaaaaaa tcttgatgct
    50341 aagggtaatc aggccacctg gtcagcggaa ggcgatgtct cttccaatga cgatctctat
    50401 acctgggtcg gtcagttggc agattacgaa ctgctcgaac agacctggac gaaagataaa
    50461 gcggtaaaat tctcggcgat gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg
    50521 aacttttact cttttcaggc ggcagccagc gatcgtgggc gggtggtcga cgatatcaaa
    50581 acgaataata aatatctgat cgtgaatagc gaagatttca attatcgctt tagtcagctt
    50641 gaatctgcgt tgaataccca gaagaactcg attcctgcgc tggaaaagga agtgaaagcg
    50701 cttgataagc aaatggtggc ggcgcagaaa gcagcggatg catattgggg aaaagatgca
    50761 aacggtaagc aaatgacccg cgaagacgcc tttaaaaaaa ttcaccaaca gcgtgatgag
    50821 tttaataaac agaacgatag cgaggcgttc gcggttaaat atgacaaaga ggtttatcaa
    50881 ccggcgattg cggcatgtca taaacagagt gaagagtgtt atgaagtgcc gattcagcag
    50941 aaacgagatt tcgatatcaa cgaacaacgg cgacagactt ttctgcaatc acaaaaactt
    51001 agccgtaaat tgcaggatga ctggataacg ctcgaaaaag ggcaatatcc gctaacgatg
    51061 aaagtttcag aaataaacag taaaaaagtc gcgattttga tgaaaattga cgatatcaac
    51121 caggctaatg aacgctggaa aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag
    51181 taagttttga tatagcgcac gatttgtagc aggatgcggc gttgaccccg catccggcaa
    51241 tcaacgtctg atgcgacgct agcgtgtctt atcagaccta caacagctgc cacatgtcgg
    51301 ctgaggtgct cacgccgcat ccggcaatca acgtctgatg cgacgcttgc gcgtcttatc
    51361 agacctacaa ccgttgccac atgtaggctg gataaggcgt tcacgccgca tccggcaata
    51421 caagtttact cagccagaaa aagctccagc agcgaattta aaaacagctt cccatgttcc
    51481 gtaatctgcc agtaatccgc acattcggtg agataaccct gggcaatcgc ctcgtctaac
    51541 tgtgggcgaa tcacatcttc gcaaagcccg gtatacgcac taaactccac gcgcggcgca
    51601 ggttccagca accggaagcg attcataaag aactcaaacg gcttatctgc ggcttcgaca
    51661 tcacgctggc tttccagata ccttccctgc ataaaaccac gcggatgacg cgttttggtg
    51721 gtacgcagaa tgcgcccatc cgggaaggtc actttgccgt gcgcgccgca gccaataccg
    51781 atgtagtcac caaagcgcca gtagttgaga ttgtgctggc actgataacc gggtttggcg
    51841 taagcggaag tttcatattg ctgataaccc gctgcggtta ataactgatg cccctgttcg
    51901 aaaatatccc acagcgcgtc gtcgtccggc agtaccggcg ggcgtgaacc aaacagcgta
    51961 ttaggttcga tggtcagttg ataccaggaa agatgcggcg gattcagttc aatagcctgg
    52021 cgcagatcgc caagcgcctc ttccagcgat tgatccggta gcccatgcat caaatcaagg
    52081 ttaaagctac gtaaccctaa accgctcgcc agctttgccg ctcgtttcgc ttcttgcggg
    52141 ccatgaatac gtcccagccg tttcagcttt tcttcgctaa aactctgtac cccaatagag
    52201 atacgattta caccggcacg ctgataatca acaaagcgat cggcttctac cgtaccgggg
    52261 ttcgcttcca tggtaatttc tgcatccgct gccagcggca aacgcgcacg cacgccgtcc
    52321 agcagcgttt gcatcgccga gccggaaagc aggctcggcg taccaccgcc aataaagatt
    52381 gtctttactt cacggccctg agcgtaagcc acatcgttgt ccagatcgtt aagcagatgc
    52441 tgaacgtaat cgtcgtgcgg cacttctcct ttcaacgcgt gagagttgaa atcgcagtac
    52501 gggcatttct gcacgcacca cgggatgtga atgtagagac tcagcggcgg taatttaacc
    52561 attacgtaaa gcgtccagca gcagtttcaa tgcctgacca cggtgggaaa tggcgctctt
    52621 ttcttcgcga gtcagttcgg cagcggtttt cccttcggaa ggaacgaaga agattggatc
    52681 ataaccaaag ccgccagtac cggccggttc acgagtaatc acaccaggcc agctaccgtg
    52741 gcacaccagc ggagtcggat cttccgcgtg acgcagatat accagcacgc agtggaaacg
    52801 cgcctgacgt tgttcgtccg gtacgtcttt cagtgtttcc agcagttttt gcagattctt
    52861 ttgatcggtc gcgtcttcac cggaataacg cgcggagtaa atgcctggcg cgccaccaag
    52921 tacatctacc gccagaccgg agtcgtcggc aattgccggt aaaccggtca cttttgccgc
    52981 atggcgcgct ttcagaatcg cgttttcgat aaaggtcagg ccggtttctt cagcggaatc
    53041 tacgccgagg tctgtttggg ccacgatatc aagaccgaag tcgctaagca gcgacgccag
    53101 ctcacgcact ttaccggcat tgccggttgc gaggacaact ttttgcatag gatacctaat
    53161 taattaacgc cgcgatttct ggcgggattt gttgcggatt aatgatttta atttgtttgt
    53221 ggcggccaag ttcgcctttt tcaatcacca cctggctttt ggcaacccgg aattgcttac
    53281 cgagaaactt caccagatgg ctgttggcct gaccgtcaac cggcggcgcg gtaatggcga
    53341 ctttaacttc gtcgccatgt aaaccgacaa tagaatcacg gctggctttc ggctgaatat
    53401 aaagccgtaa aaccagaccg tcatcgttaa ctgttacggc actcataacg ccatccacag
    53461 ccccggcagc agcatgtttc cggttgcctg taatacttct gcgacaccca tattgatgac
    53521 atacagcagc agaacgagga tcatcggcga gaaatcaatc ccacccattg ccggtagcag
    53581 gcggcgaatc gggcgcagca gcggatcggc cagctgaatc agcacgtatt caatcgggct
    53641 acggccctgg cttacccagc tcataatcgc catcaccagc aggacccaga aaatcagcag
    53701 gccgatggtt ttcagcagaa tcagtaaacc ggcaatccag atgattggca ggaaggtcac
    53761 cactttaaac agcacgatgg ctttgataaa actgagaata taggcaacca gtagcgaggc
    53821 gctatcaatg ggtcccattg ccggaataac gcggcgcagt ggcccgataa ttggctgcgt
    53881 taccttcact acaaactgtg agaaggggtt gtaaaaatca caatgagccc actgcatcca
    53941 gatgcgtaat aacagcacca tggtatacag ctcaatgacc gttgaaagca ggaaagtcaa
    54001 cgtattcatg gcgttccttt aattccttat tttttagagt aatcacgcgc accaaaaatt
    54061 gcagtgccga tacgaaccat cgtgctacct gccgcaatgg cggcttccat atcgtccgac
    54121 attcccagag agagcgtgtc gatatgcggg tagcgcgttt tcagtccggc aaatgctaca
    54181 gccatttgac gtgcaacttc aaactgcctt acatattctg actcaggcgc agggattgcc
    54241 atcagcccgc gcagacgtaa acgcggtagt tcagcgaccg cagctgccag ctcgtccagt
    54301 tctgccagtt gaatcccgga cttactgttt tcatcactaa tattaatttg aatcagcacg
    54361 ttaagagggg gaagttctgc cgggcgctga tcgttgaggc gggtagcgat gcgcaaacgg
    54421 tcgatggtat gacaccagtc gaaatgctct gccaccaggc ggcttttatt agactgcaac
    54481 gggccaataa aatgccattc taatcctgtt acgcccagtt cctgaaagtg gcgaatttta
    54541 tctacccctt cctgaacgta gttttcacca aattgacgct gcccggcatc aatggcttct
    54601 gcgatggcgc tcgcaggttt tgttttactg actgcaagca gcgtaatttc ttctggagaa
    54661 cggccgcaac gcgttgcagc cgctgagatt ttgtcccgga cctgtgccag gttatgcgca
    54721 atatcgttca ttttccgagg atcttagtat gaatatggaa gaaattgtgg cccttagtgt
    54781 aaagcataac gtctcggatc tacacctgtg cagcgcctgg cccgcacgat ggcgcattcg
    54841 cgggctaatg gaagctgcgc cgtttgatgc gccggacgtc gaagagctac tgcgggagtg
    54901 gctggatgac gatcagcggg caatattgct ggaaaatggc cagctggatt ttgccgtgtc
    54961 gctggcggaa aaccagcgat tgcgcggcag tgcgttcgca caacggcaag gtatttctat
    55021 ggcgttacgg ttgttacctt cgcactgccc gcagctcgaa cagcttggcg caccaacggt
    55081 attgccggaa ttactcaaga gcgagaatgg cctgattctg gtgacggggg cgacggggag
    55141 tggcaaatct accacgctgg cggcgatggt tggctatctc aatcaacatg ccgatgcgca
    55201 tattctgacg ctggaagatc ctgttgaata tctctatgcc agccagcgat gtttgatcca
    55261 gcagcgggaa attggtttgc actgtatgac gtttgcatcg ggattgcggg ctgcattgcg
    55321 ggaagatcct gatgtgattt tgctcggaga gctgcgtgac agcgagacaa tccgtctggc
    55381 gctgacggcg gcagaaaccg ggcatctggt gctggcaacc ttacatacac gtggtgccgc
    55441 gcaggcagtt gagcgacttg tggattcatt tccggcgcag gaaaaagacc ccgtgcgtaa
    55501 tcaactggca ggtagtttac gggcagtgct gtcacaaaag ctggaagtgg ataaacagga
    55561 aggacgcgtg gcgctatttg aattgctgat taacacaccc gcggtgggga atttgattcg
    55621 cgaagggaaa acccaccagt taccgcatgt tattcaaacc gggcagcagg tggggatgat
    55681 aacttttcag cagagttatc agcagcgagt gggggaaggg cgtttgtgac gttgccatgc
    55741 ccgatgtgca agcttatcgg gcctacaaag gcaataaacg tctgatgcga cgcttgcgcg
    55801 tcttatcaga cctacaaccg ctgccacatg taggccggat aaggcgttca cgccgcatcc
    55861 ggcaataaga cgcgatgata ttatttacac tccgccaggc ttttaaatcg ccttaatatc
    55921 cctgctcgaa atagctttcg agaataataa ccgcagaggc agagtcgact ttgcctttgt
    55981 taagcgcccg atagccgccc tgttcaaaca gaccggaacg ggcttccacg gtgctaagac
    56041 gctcgtcatg gagctttacg tcaacaccga aacgaccatg aatacggttg gcaaatttac
    56101 gcgctctggc ggttaacggc tgttcggtac cgtccatatt cagcggcaaa ccgacgatga
    56161 tttcgtccgg ctgccactct ttcagtaaac gctcgataat gttccagtcc ggcgtaccgt
    56221 cctgtgcttt aattgcaggc aaagggcgag cggtgccggt aatgcgttgg ccgaccgcta
    56281 cgccaatgct tttggtgccg aagtcgaagg cgagtaaggt tccactcatc aggcgtgccc
    56341 cgcaacgcca ggcatggtga gaatatccac accaatcagt tttgccgcct cgcgccagcg
    56401 gtcggcaatc ggcgttttga acagaatatt cagatctgcc ggggcggtta gccacgcgtt
    56461 atcgagaatt tcttgttcca gttgaccttt ctcccaggag gcatagccca gagccaccaa
    56521 tacgtcagac ggttgtttat cggtgccgag cgtttccagc acatcgcggg aagtggtcat
    56581 caccgtgttg tctgaaatgc gaatgctgga agcaaaattg gagggcggag tatgcaaaat
    56641 aaacccgcga tcttcagcca gtgggccgcc gagcataacc ggtttatcca gacggattga
    56701 ttcatcacgc ggctccggcg tgatcttcag cttttccaga atcccttcaa ttttgagatt
    56761 ttccagcggc ttgttgacga tgatccccat tgcaccattg gtattatgtt cgcaaatgta
    56821 gaccacggaa cgacggaaaa tcggatcctg gagagcaggc atggcaataa gaaagtgatg
    56881 ctgtaaattc attgtcagag gttctgtttc ctggttcaaa aaagcaacag cgcccagtat
    56941 gcgtggaaag aagggcgctg tcactcagag tctcaacgag atccttctcg ctaaggtggg
    57001 ttactgctgc tgtaaacgtg cttcgatggc atccattaac attccggtga tcgacaccgg
    57061 aaactctgct tcaatctcac gaatacaggt tgggctggtg acgttaattt cagtcagacg
    57121 gtcgccgatg atatccaggc caacaaaaat cagccctttt tctttcaacg tcggcccgat
    57181 ctgacgggcg attttccagt cactttccgt cagcggacga ggttcaccgc gaccaccggc
    57241 agccagattg ccacgggttt cgcccccctg cggaatacgc gccaggcagt acggtaccgg
    57301 ctcgccatcc accaccagca cgcgtttgtc gccatcttta atggctggca ggtaattttg
    57361 cgccatgcag tagcgagtgc catgctcagt cagggtttcg gcaatcacgc cgaggtttgg
    57421 atcgccttct ttcacgcgga aaatcgacgc gccgcccata ccgtccagcg gcttaagaat
    57481 gatgtcgctg tgtttctccc agaacgcttt tagctgcgct ttattgcgcg taaccagcgt
    57541 ttctggcgtt aagtcagaga accaggcggt aaacagtttc tcgttacagt cgcgcaggct
    57601 ctgcggcttg ttaacgatca gcgtcccttt ctcttcggca cgttccagaa tataggtcgc
    57661 gtagataaac tcggtatcaa acggcgggtc tttacgcatc aggatcacat cgagatcggc
    57721 cagcggcaga tcctgttcac cgacgaatga aaaccactct tcgtagttct gcttcacgtt
    57781 cagcgtgcgg gtatgggcgc gggcttcgcc attgatcaga tacagatcgc ccatctccat
    57841 atagtgaagt tcgtaaccac gacgctgtgc ttccagcaac atagcgaaac tggaatcttt
    57901 cttgatgttg atgtttgcga tggggtccat cacgatgccg agcttgatca ttattcttct
    57961 ccgttagccc aaatcgccaa atcgtacttg tagcgcggta atggcggtga gcgcagttgt
    58021 ctctgtacgc aaaacgcgag gtcccaacag gatatcagta aattgatagc gggcagtcat
    58081 ggcaatttca tctgccgata aaccgccttc cgggccaatc agcaggcgga cgcgttcaac
    58141 cggtaacggc aacgtattga tgctgttact ggcgcgcggg tgaagattca gtttcagtcc
    58201 ttcatcctgc tctgcacacc aggcttccag atccatcgct ggacggattt ccggcacccg
    58261 gttacgacca cactgctcac aggcagcaat tgcaatcttc tgccactgct ggagcttctt
    58321 gttcagacgt tcactatcca gtttaacgcc gcagcgctca gaaaaaagtg gcgtaatgag
    58381 gcttacaccg agttcgatcg atttctggat cgtaaattcc attttttcac cacgcgacat
    58441 cacctgaccg aggtgaatat gcagcggaga ttcgcgatcg tcgatctggc cttccagcac
    58501 cttcacttcc acgctttttt tgctggcgct ggtaatttcg gcgtcaaaga cctggttgct
    58561 accgtcaaac aattgcaacg cctgccccgg ccccatgcgc agtacgcgcc cgatatggtt
    58621 ggcggcatct tcgcaaagcg cgatgtgaga atggctggtc agtggttcag gatgataaat
    58681 gcgggggata cgcatagtta aaaatccgcg tcgtctcccc acgcggttgt tcgcgtgggg
    58741 tagggtttaa caaaaagaat cccgctagtg taggttagct ctttcgcgcc tggcaagcgc
    58801 gttgcacata cgggttatga ttgccctgca ccttcgcgat gcgttcatcg cgctcgcact
    58861 cccagtcggt aaccggatac atcttgttcc atgcgttgaa cagctgcgtt tgctggcgag
    58921 agtgtgtcag gttgtattgg tcacgcatat agaagtaggt acgcgcaatg gcaccgcgtg
    58981 cacgcgctgg tggttcggca gctttttctt tgaaatcgac cttcatggcg cattgaccgt
    59041 actggccttc gccgccattc cactggctgt acataaagtt gccgcgatcg ccattcacct
    59101 cgccgactga cggctgcagg ttatgcatat cgctttccat cttgcgatag accggatctt
    59161 tagcgcagtt tttacgtcca ccgtcctgcc agcactggcg ctggtgaccg aattgccagg
    59221 cgggaacgac gtgttcccac tctacgcggc tggcgcggtt ttcatttttg cgcacctgat
    59281 agccgcacga ttgcagatca acaacgcctt ttttgccctg ccagttaatt ttacatccgc
    59341 aataaaacgt accgggcgcg tcagcgtgga cttttaccgc cgcggctttc gcctgagaaa
    59401 aactattgat accttcggcc aacgccgggc cggaaaatgc tgcgctcagt accaccgcag
    59461 caatagacaa ataacggtac atcacttact ccgtgttaaa acgagccagc aacgtagcga
    59521 aagttgtttt ggtatgcaat cagccagatc aggaaagttc ctgatagttc aggtggttat
    59581 ttcgcaacca gctgttcacc gcagtgaaca caccgataga cggcctcgcc acgaactacg
    59641 cgattatggc ggcgtacggt aagctgatgt tcctggcact tacagcggta ggggaaggtg
    59701 ttgcgacgca cggattgcag ttcgaactga tgcgtacgac gggcgggaac gcccagcacg
    59761 ctttccatca tccacttcca ctctttgcca tgtggcgcta cgcggccgaa atgtttccat
    59821 accagcaaat gtgccagttc gtgcggcacc acttcttcaa taaaggcttc actgttttcc
    59881 aacagcaaaa cgggattgag gcgaatttca tagctttcca gccaggctgt tccggcggag
    59941 gttccgcgct gggtgtaaga gagttttggc tccgggtagt tacgccctag cttcaggttg
    60001 gccagggcga gtttttcccg caggcgacgc ataacggcct gttggatggc gatagggaga
    60061 cgggatgttt tcatagcggc agaggataga gcgaagaaaa gatgactgca agaggcggct
    60121 tcctccgcga cgggaggaag cctgaggaga ttaatcgtga gcgccgattt cgcgcagttt
    60181 acgacctttc atcagattac gttcaatatg ttccagcgaa acgtgtttgg tttccggtac
    60241 cagccacaat gtcagcagga taaacagtac gttcagagcc gcatacaccc agaaggtgtt
    60301 ggcgttaccc agcgtgttga gcatggtcag gaacgttgcg ccaacgatca tgttggcaat
    60361 ccagttggtg gcggtggagc aggtgatgcc aaaatcgcgg cctttcagcg gctgaatttc
    60421 ggagcacagt acccaaatca gcggaccggc actcatggca aaaccgacaa taaacatcag
    60481 cagcatggcg atggcgaaat actgcgccga cggagagtgg ataccgatat gcatcattgt
    60541 accgagtacg cccataccag cagccatcac caggaagccc agcgttagcg ttggtttacg
    60601 tccccagcgg tcaacaaggc cgattgcgat aaaggtggca agtacgttgg tcaggccgac
    60661 aatcactgtc ccccacattt gctcggtggt gttggtatat cccgccagtt cgaagatttt
    60721 cggcgcgtaa tacatgatga cgttcatccc ggtgaattgc tgcattacct gtaacagtac
    60781 gccaaggaac accgcgcggc ggaagttgct attctcttta aacagcgccc agccactctg
    60841 tttaacctgc aaactttcac ggatttcatc cagttcgcgt ttcgcttccg cgctggtgtc
    60901 acgcaggcgt agcagcacgc gttcggcatc aacaaaacgg cgtttggcgg caaaccaacg
    60961 tgggctgtct ggcaggaaga agacaccaat cagcagcaaa attgccggga tgataatcac
    61021 acccagcatc cagcgccatg caccggtgta gctgaaggcg gtatcagaaa gataagcacc
    61081 gaggatcccg atagtgatca ttaactgata catcgagatc atactgccgc gaattttttc
    61141 cggcgcaatt tcagagaggt acagtggtgc ggtataagag gccacaccta ccgccagccc
    61201 cagcagaacg cgggaaagaa tcagtacttc aacgtttggc gcagccgcag agaacagcga
    61261 accggcaaca aacaaaattg cgccgatcat caggctcttt ttgcgcccga gtttaaagga
    61321 gagccagccg ctgcccaccg caccgactgc cgcaccgaac atcatggagc ttacgaccca
    61381 ttcttgcgtg tgcgaagtaa tctggaattc atctgcaata aacggcagtg cgccagcaat
    61441 tacaccgata tccaggccaa agagtaatcc cgccagagcg gcaaggaagc agacgaaaaa
    61501 cgtcattgcc ttgtttgacc gcccctgttt tttagcgtca ggcatgatgc cctccaatat
    61561 ggttattttt tattgtgaat taagataggt gagtacgacg taaaaagatg tgaagcaaat
    61621 cacatcagtg taatcgctta cactaacatt tatctta
//
LOCUS       DAPGRN010000014        60839 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPGRN010000014 DAPGRN010000000
VERSION     DAPGRN010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.014"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..538)
                     /locus_tag="RHW33_000972"
                     /pseudo
     CDS             complement(1..538)
                     /locus_tag="RHW33_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            546..1088
                     /gene="tnpA"
                     /locus_tag="RHW33_000973"
     CDS             546..1088
                     /gene="tnpA"
                     /locus_tag="RHW33_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV1644511.1"
                     /translation="MILWSMNKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDYDATEK
                     LRTYFSNVCADFEAELVEMDGEPDHVHLLINYPSKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRINKKRQGYLSCRLSFMR"
     gene            complement(1089..2504)
                     /gene="gltX"
                     /locus_tag="RHW33_000974"
     CDS             complement(1089..2504)
                     /gene="gltX"
                     /locus_tag="RHW33_000974"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV1644512.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            2763..2838
                     /locus_tag="RHW33_000975"
     tRNA            2763..2838
                     /locus_tag="RHW33_000975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2796..2798,aa:Val,seq:tac)
     gene            2883..2958
                     /locus_tag="RHW33_000976"
     tRNA            2883..2958
                     /locus_tag="RHW33_000976"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2916..2918,aa:Val,seq:tac)
     gene            3005..3080
                     /locus_tag="RHW33_000977"
     tRNA            3005..3080
                     /locus_tag="RHW33_000977"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3038..3040,aa:Val,seq:tac)
     gene            3085..3160
                     /locus_tag="RHW33_000978"
     tRNA            3085..3160
                     /locus_tag="RHW33_000978"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3118..3120,aa:Lys,seq:ttt)
     gene            3191..3316
                     /locus_tag="RHW33_000979"
     ncRNA           3191..3316
                     /ncRNA_class="other"
                     /locus_tag="RHW33_000979"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            3436..3777
                     /locus_tag="RHW33_000980"
     CDS             3436..3777
                     /locus_tag="RHW33_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HDV1644513.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTGGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(3768..4694)
                     /locus_tag="RHW33_000981"
     CDS             complement(3768..4694)
                     /locus_tag="RHW33_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1644514.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAAEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4784..5782
                     /locus_tag="RHW33_000982"
     CDS             4784..5782
                     /locus_tag="RHW33_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV1644515.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(5779..5997)
                     /locus_tag="RHW33_000983"
     CDS             complement(5779..5997)
                     /locus_tag="RHW33_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV1644516.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5999..8014)
                     /gene="ligA"
                     /locus_tag="RHW33_000984"
     CDS             complement(5999..8014)
                     /gene="ligA"
                     /locus_tag="RHW33_000984"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV1644517.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKQWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKSKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVYNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIDVIDEAEMLRLLGS"
     gene            complement(8085..9083)
                     /gene="zipA"
                     /locus_tag="RHW33_000985"
     CDS             complement(8085..9083)
                     /gene="zipA"
                     /locus_tag="RHW33_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV1644518.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9313..10074
                     /gene="cysZ"
                     /locus_tag="RHW33_000986"
     CDS             9313..10074
                     /gene="cysZ"
                     /locus_tag="RHW33_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV1644519.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10259..11230
                     /gene="cysK"
                     /locus_tag="RHW33_000987"
     CDS             10259..11230
                     /gene="cysK"
                     /locus_tag="RHW33_000987"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV1644520.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11614..11871
                     /gene="ptsH"
                     /locus_tag="RHW33_000988"
     CDS             11614..11871
                     /gene="ptsH"
                     /locus_tag="RHW33_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV1644521.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11916..13643
                     /gene="ptsI"
                     /locus_tag="RHW33_000989"
     CDS             11916..13643
                     /gene="ptsI"
                     /locus_tag="RHW33_000989"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV1644522.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13684..14193
                     /gene="crr"
                     /locus_tag="RHW33_000990"
     CDS             13684..14193
                     /gene="crr"
                     /locus_tag="RHW33_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV1644523.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14235..15086)
                     /gene="pdxK"
                     /locus_tag="RHW33_000991"
                     /gene_synonym="thiJ"
     CDS             complement(14235..15086)
                     /gene="pdxK"
                     /locus_tag="RHW33_000991"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV1644524.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVTADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15191..15559
                     /locus_tag="RHW33_000992"
     CDS             15191..15559
                     /locus_tag="RHW33_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV1644525.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGYEHTCDEAVSHLRLKLGNTRNRINTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15562..16473)
                     /gene="cysM"
                     /locus_tag="RHW33_000993"
     CDS             complement(15562..16473)
                     /gene="cysM"
                     /locus_tag="RHW33_000993"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV1644526.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16607..17704)
                     /gene="cysA"
                     /locus_tag="RHW33_000994"
     CDS             complement(16607..17704)
                     /gene="cysA"
                     /locus_tag="RHW33_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV1644527.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17694..18569)
                     /gene="cysW"
                     /locus_tag="RHW33_000995"
     CDS             complement(17694..18569)
                     /gene="cysW"
                     /locus_tag="RHW33_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV1644528.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEENHEH"
     gene            complement(18569..19402)
                     /gene="cysT"
                     /locus_tag="RHW33_000996"
     CDS             complement(18569..19402)
                     /gene="cysT"
                     /locus_tag="RHW33_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV1644529.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLAATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(19402..20418)
                     /gene="cysP"
                     /locus_tag="RHW33_000997"
     CDS             complement(19402..20418)
                     /gene="cysP"
                     /locus_tag="RHW33_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV1644530.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(20576..21367)
                     /gene="ucpA"
                     /locus_tag="RHW33_000998"
     CDS             complement(20576..21367)
                     /gene="ucpA"
                     /locus_tag="RHW33_000998"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV1644531.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21496..22353)
                     /gene="murR"
                     /locus_tag="RHW33_000999"
     CDS             complement(21496..22353)
                     /gene="murR"
                     /locus_tag="RHW33_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV1644532.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDMESLKMIQR
                     SSELTQRLK"
     gene            22517..23413
                     /gene="murQ"
                     /locus_tag="RHW33_001000"
     CDS             22517..23413
                     /gene="murQ"
                     /locus_tag="RHW33_001000"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV1644533.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23417..24841
                     /gene="murP"
                     /locus_tag="RHW33_001001"
     CDS             23417..24841
                     /gene="murP"
                     /locus_tag="RHW33_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV1644534.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24846..26150
                     /gene="pbp4b"
                     /locus_tag="RHW33_001002"
     CDS             24846..26150
                     /gene="pbp4b"
                     /locus_tag="RHW33_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV1644535.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGINLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDLIAKYIPGFADSPSDTIKGKNTLRIADLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(26208..27107)
                     /gene="yfeX"
                     /locus_tag="RHW33_001003"
     CDS             complement(26208..27107)
                     /gene="yfeX"
                     /locus_tag="RHW33_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV1644536.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(27203..27778)
                     /locus_tag="RHW33_001004"
     CDS             complement(27203..27778)
                     /locus_tag="RHW33_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV1644537.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRVVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27839..28288)
                     /locus_tag="RHW33_001005"
     CDS             complement(27839..28288)
                     /locus_tag="RHW33_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV1644538.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLTFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGFGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(28275..28700)
                     /locus_tag="RHW33_001006"
     CDS             complement(28275..28700)
                     /locus_tag="RHW33_001006"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV1644539.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28914..29783
                     /gene="amiA"
                     /locus_tag="RHW33_001007"
     CDS             28914..29783
                     /gene="amiA"
                     /locus_tag="RHW33_001007"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV1644540.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29787..30686
                     /gene="hemF"
                     /locus_tag="RHW33_001008"
     CDS             29787..30686
                     /gene="hemF"
                     /locus_tag="RHW33_001008"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV1644541.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREGGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30692..31744)
                     /gene="eutR"
                     /locus_tag="RHW33_001009"
     CDS             complement(30692..31744)
                     /gene="eutR"
                     /locus_tag="RHW33_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV1644542.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31790..32290)
                     /gene="eutK"
                     /locus_tag="RHW33_001010"
     CDS             complement(31790..32290)
                     /gene="eutK"
                     /locus_tag="RHW33_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV1644543.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWSLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32303..32962)
                     /gene="eutL"
                     /locus_tag="RHW33_001011"
     CDS             complement(32303..32962)
                     /gene="eutL"
                     /locus_tag="RHW33_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV1644544.1"
                     /translation="MPALDLIRPSVTAMRVIASVNADFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAQDTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32972..33859)
                     /gene="eutC"
                     /locus_tag="RHW33_001012"
     CDS             complement(32972..33859)
                     /gene="eutC"
                     /locus_tag="RHW33_001012"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV1644545.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33880..35241)
                     /gene="eutB"
                     /locus_tag="RHW33_001013"
     CDS             complement(33880..35241)
                     /gene="eutB"
                     /locus_tag="RHW33_001013"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV1644546.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(35253..36656)
                     /gene="eutA"
                     /locus_tag="RHW33_001014"
     CDS             complement(35253..36656)
                     /gene="eutA"
                     /locus_tag="RHW33_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV1644547.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVGAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36653..37879)
                     /gene="eutH"
                     /locus_tag="RHW33_001015"
     CDS             complement(36653..37879)
                     /gene="eutH"
                     /locus_tag="RHW33_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV1644548.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(37902..38093)
                     /locus_tag="RHW33_001016"
     CDS             complement(37902..38093)
                     /locus_tag="RHW33_001016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644549.1"
                     /translation="MPDATLAASYPTWGIAHVGRIRLHAASASGAVQLPDATLAASYP
                     AYGIAHVGRIRLHAASANK"
     gene            complement(38094..39281)
                     /gene="eutG"
                     /locus_tag="RHW33_001017"
     CDS             complement(38094..39281)
                     /gene="eutG"
                     /locus_tag="RHW33_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV1644550.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAELHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(39271..40107)
                     /gene="eutJ"
                     /locus_tag="RHW33_001018"
     CDS             complement(39271..40107)
                     /gene="eutJ"
                     /locus_tag="RHW33_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV1644551.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(40118..41521)
                     /locus_tag="RHW33_001019"
     CDS             complement(40118..41521)
                     /locus_tag="RHW33_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV1644552.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     IAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAGKIA
                     AAIGLKVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41533..41820)
                     /gene="eutN"
                     /locus_tag="RHW33_001020"
     CDS             complement(41533..41820)
                     /gene="eutN"
                     /locus_tag="RHW33_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV1644553.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41927..42220)
                     /gene="eutM"
                     /locus_tag="RHW33_001021"
     CDS             complement(41927..42220)
                     /gene="eutM"
                     /locus_tag="RHW33_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV1644554.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42259..43275)
                     /gene="pta"
                     /locus_tag="RHW33_001022"
     CDS             complement(42259..43275)
                     /gene="pta"
                     /locus_tag="RHW33_001022"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV1644555.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIM
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTE
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEMNRESSLQ
                     TLVE"
     gene            complement(43272..44075)
                     /gene="eutT"
                     /locus_tag="RHW33_001023"
     CDS             complement(43272..44075)
                     /gene="eutT"
                     /locus_tag="RHW33_001023"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV1644556.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLGHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKSPLTVNQIQQRLGETQ"
     gene            complement(44072..44773)
                     /gene="eutQ"
                     /locus_tag="RHW33_001024"
     CDS             complement(44072..44773)
                     /gene="eutQ"
                     /locus_tag="RHW33_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV1644557.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDELIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWDNAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44748..45227)
                     /gene="eutP"
                     /locus_tag="RHW33_001025"
     CDS             complement(44748..45227)
                     /gene="eutP"
                     /locus_tag="RHW33_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV1644558.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45240..45575)
                     /gene="eutS"
                     /locus_tag="RHW33_001026"
     CDS             complement(45240..45575)
                     /gene="eutS"
                     /locus_tag="RHW33_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV1644559.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45869..48148)
                     /gene="maeB"
                     /locus_tag="RHW33_001027"
     CDS             complement(45869..48148)
                     /gene="maeB"
                     /locus_tag="RHW33_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV1644560.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48437..49387
                     /gene="tal"
                     /locus_tag="RHW33_001028"
     CDS             48437..49387
                     /gene="tal"
                     /locus_tag="RHW33_001028"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV1644561.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49407..51410
                     /gene="tkt"
                     /locus_tag="RHW33_001029"
     CDS             49407..51410
                     /gene="tkt"
                     /locus_tag="RHW33_001029"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV1644562.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKKAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLIFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRKSVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(51519..52562)
                     /locus_tag="RHW33_001030"
     CDS             complement(51519..52562)
                     /locus_tag="RHW33_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV1644563.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(52688..53263)
                     /gene="nudK"
                     /locus_tag="RHW33_001031"
     CDS             complement(52688..53263)
                     /gene="nudK"
                     /locus_tag="RHW33_001031"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV1644564.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(53331..55310)
                     /gene="aegA"
                     /locus_tag="RHW33_001032"
     CDS             complement(53331..55310)
                     /gene="aegA"
                     /locus_tag="RHW33_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV1644565.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            55516..57216
                     /gene="narQ"
                     /locus_tag="RHW33_001033"
     CDS             55516..57216
                     /gene="narQ"
                     /locus_tag="RHW33_001033"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV1644566.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            57380..60493
                     /gene="acrD"
                     /locus_tag="RHW33_001034"
     CDS             57380..60493
                     /gene="acrD"
                     /locus_tag="RHW33_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV1644567.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPKLTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60592..60651)
                     /gene="ypfM"
                     /locus_tag="RHW33_001035"
     CDS             complement(60592..60651)
                     /gene="ypfM"
                     /locus_tag="RHW33_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV1644568.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14130 a        16797 c        15612 g        14300 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta
       61 ccacttaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 gttccggtag cgcatccagc caagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcatc attctgtccc cgcttttttg aatcggggaa
      241 aagcagcccg cttccggaag aagtttttgt aagcccgctc aaggtctttc agtgactgtt
      301 gcaatggctg tgagggcgaa tctttaagcc attgcgtttc agtggcgttt ttccactcaa
      361 ccagccagga agccattttc ccgtaaggga tgtatttatt accggcctca tgattctcat
      421 tctgaagcgc cagaacacga ttgaaaacga aacgacatgc gcctgcgaag cgcctcattt
      481 cacgctcctg ttgaccacct ggtcttaact ggaatttaaa tgcctgtagt cgtttcaccg
      541 gccaaatgat actttggtct atgaataaag aaaccgatat ccgacgggga aggcattgtg
      601 ttttcctgat gcatgttcat ctggtctttg tcaccagata ccgacgtcag atttttgact
      661 atgacgcgac agaaaaacta cgcacttact tttcaaatgt atgtgctgat tttgaagctg
      721 aactggttga aatggatggc gaaccagatc acgtccattt gttaattaac tatccttcca
      781 aactggcgat atccagtctg gtaaacagcc tcaaaggcgt atcgggtagg ttactgcgac
      841 gagatcgacc agatattgca gttaggtatt actacaaagg cgttttgtgg agtcctggct
      901 attttgccag tagctgcgga ggtgcgccaa tatccgtcat ccgccaatac attgaacaac
      961 agcaaacacc tggtcaggtg gaaaaccgcg ccttatatcc ccgccctgaa ggacggggtt
     1021 ttacggcgca ccggataaat aaaaaacggc aggggtatct ctcctgccgt ttatctttta
     1081 tgcgctaatt actgctgatt ttcgcgttcc gcaataaaag ccagcgcttt gttgatacgc
     1141 tcgatactgc gggtcttacc gatcgcgtga acggtaacgt ccagcgccgg agactgcccc
     1201 gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac ttccagctca
     1261 tccgccgtcg cctgaatagc gtgatgaacg ttttcagctg tccagtcagt aatcgcggcc
     1321 agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg ttttttcgcc
     1381 gcatcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg tgccatctct
     1441 ttcagcgtct tgcagcgttc acccagcagt ttcaccagat cagccagctg cgggccgtta
     1501 cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagctac atactccggc
     1561 ggcagcgcgt taatgtagtg atggttcagc cacagcagct tgtcggtgtt gaacgcactg
     1621 gcagatttgc tgacggcatt caaagtgaag tatttgatca tctcttcacg agtgaagatt
     1681 tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc ttctggcaaa
     1741 taaccgtcat cacgatactg cattacgctg actgccccgt gacgtttgga cagttttttg
     1801 ccgtcatcgc cgttgatcat agaaacgtgc gcataaaccg gtaccggcgc tttcagggct
     1861 ttaagaatgt tgatctggcg tggcgtgttg ttgatatggt cttcgccacg gataacgtgg
     1921 gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg ggaaccatcg
     1981 gtacggcgga taataagatc gtccagttcc tggttgctga actcgatcgg accacggatc
     2041 tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacgac acacggctca
     2101 tcatcagcgt gatgctcatg actgtggcgg cagcgaccgt cataacgcgg cttctcacct
     2161 ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata gcatttataa
     2221 gcagtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa acgtttggtc
     2281 tggtagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc cataatggct
     2341 tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag cacgaactca
     2401 ccgccgtgat tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc gccaacgtgc
     2461 agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg gccttacgtt
     2521 tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata ttctatcact
     2581 cccgcctgat tcctcaatgt agttcgggtt gcaaccttac gcattattgt tttcgttgac
     2641 aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac gaacgaacaa
     2701 tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac tccacacagc
     2761 gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
     2821 tcccgtcatc acccaccaac tactatatgt agtctccgcc gtgtagcaag aaattgagaa
     2881 gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc ggcggttcga
     2941 tcccgtcatc acccaccact ttctcaccag ctaaatttct tgtaaaaatg tgaagtaccg
     3001 aagtgggtga ttagctcagc tgggagagca cctcccttac aaggaggggg tcggcggttc
     3061 gatcccgtca tcacccacca cttcgggtcg ttagctcagt tggtagagca gttgactttt
     3121 aatcaattgg tcgcaggttc gaatcctgca cgacccacca atgtaaaaaa gcgccctaaa
     3181 ggcgcttttt tgctatctgc gatactcaaa gattcgaacc tgcagcaggt tcgagttgag
     3241 cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa gggcgagcga agcgagtcat
     3301 cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgatt
     3361 tgtaagattg atttttcttc tcaagttcct tgttcttcta ccgatattac ctcgtacagt
     3421 gacgaggact gaacaatggc cactatctct tcaacttcaa ttccttccat acaaacacag
     3481 agtagtaaca gggcatcaca aggtagcgat gttgcttcac aaatcgcgcg gataagtcag
     3541 caaatcatca aactcactca acaaattaaa gagattgtag acactggcgg cagtgctgaa
     3601 gacaagcaaa aacaggccga attgattcaa cagcaaatca ccctactgga aacacaactg
     3661 gcgcagttgc agagacagca ggcagaaaag gcgcaggaga aagagcagcg tttgtctctg
     3721 aatgtatccc ttctaaatcc tgttgaaaat acgactcata tcgatatcta tatctgatac
     3781 aacggatccc cttcccgacc agccattagc gcattgccct ggtctcgtac cacgtcccat
     3841 aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc gcaccagcat caactggcgt
     3901 tcaacaaccg gcgtaatgcg ctttaccacc agtgggctac cttcaggcaa tggtagcgcc
     3961 agtgcgggaa gaatactgat gccgatgcct gcggctacca tcgggaacag cgtcgccgga
     4021 tgtccaatct cctgcacaat attcgcctga ataccattgc gcgccagcgc tgcgtcaatc
     4081 agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt tcgctccctg taatgcctgc
     4141 cagggtacat aatcttccgc ggccaaagcg ctatcccgat ggcaaagcag aaagaaaggc
     4201 tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc ctggatcgat gacgatgcca
     4261 aagtcgacat ctccctggcg aatactttcc atcacccact gctgcggacg atcgtgcagg
     4321 acaaactgaa tatctggata gcggcgatgg ctttcagcaa tgcattgcgg aataagatgc
     4381 gcggaaatgg tctggctggc agcgacccga acttttccgc tcagttgttg ccccatacgc
     4441 ccggtatcgc gcaacgtgct gttaagttca tccagcagtc gctcaagacg caaagccaac
     4501 tgctgccctg catctgtaag caccacttca cgcgtggttc tgtccagcag gcgaacacca
     4561 gtatgatttt ccagctcctt cacactgtga cttactgccg actggctcag gccgatacgc
     4621 tctcctgcac gactaaaact tttctcctgc gctactgtga cgaaaacctt taattgtttt
     4681 aaagaataat tcatctgttt tcttcatgaa tggatgcaat aaatcaattt tatttctcaa
     4741 actggaagaa gcacaataga accatcgatc atctggagtc tttatgaaac tttttcgtat
     4801 cctcgatcct ttcaccttaa ccctgatcac ggtggtgctg ctggcctctt tctttccggc
     4861 cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc gcagctattg ccctgctgtt
     4921 ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt gctggcggtg gtcactggcg
     4981 actgcatttg tgggtaatgt gcagcacctt cgtgctgttt ccgattctgg gtgtactgtt
     5041 tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac tccggttttc tctacttgtg
     5101 cattctcccg gctaccgtgc agtctgcaat cgccttcacg tcaatggcgg gcggtaacgt
     5161 cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg gggattttcc tttcaccatt
     5221 gctggttggt ctggtgatga atgttcacgg tgcagggggc agccttgagc aggtcggtaa
     5281 aattatgctg caactgctgc tgccgtttgt gttggggcat ctttcccggc cgtggattgg
     5341 tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact gaccagacgt ccattctgtt
     5401 ggtggtttat acagcgttca gcgaagccgt cgttaatggt atctggcata aagttggctg
     5461 gggatcattg ctgtttatcg tggtggtcag ctgcgttctt ctggctatcg tgattgtagt
     5521 taacgtcttt atggcacgcc gactgggctt caataaggca gatgaaatta ctatcgtctt
     5581 ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg gcaaacattc tgttccccac
     5641 atcggtgatc ggtatgatgg tgctgcccct gatgattttc catcagatcc aattgatggt
     5701 ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa cagttacagg cgcagcagga
     5761 aagcagcacc gataaagctt aaagcggacg cttcaggggc tggaccagtt gcgtcagccc
     5821 ctcggtttta atcagcagcg tgatttgcat tagctcaccg agcttccctg ccgggaactc
     5881 atctttgcgg gcaaaccaca gcagatactc ctccggcagg tcgattaagc gacgcccttt
     5941 gtatttgcca aacggcatta tcgtattggc tatttcaatc agctgctctt tttccatctc
     6001 agctacccag caaacgcagc atttccgctt cgtcgatgac gtcaataccc agttcctgcg
     6061 ccttcgccag tttagatcct gcagcttcac ccgctatcac cagatcggtt ttcttcgaca
     6121 cgctgcccgc gactttcgcg cccagttcga ccagtcgagc tttagcgtca tcacgcgaca
     6181 tctggcttaa gctgcccgta agcaccacgg ttttaccagc aaacgggctg tcaatctctt
     6241 ccgcgttgat aacgatcggc gcaggccagt gaacaccttc cgccaacagc tcgctgatga
     6301 cattgcggtt gctttcttcg gcaaagaagt tgtaaacgtg ggatgcaacg acaataccaa
     6361 catcaggcac cttttgcagc tcttcaatcg aagcggcttc cagcgcttcc agcgttccga
     6421 aatatgccgc cagacctgct gcggtggcct cgccgacttc acggatgcca agtgcataga
     6481 ggaagcgagc aaaggtggtt tctttcgatt tttccagcgc gttaaccacg ttttgtgccg
     6541 attttggccc catacgctcc agtccggtaa gtttgcctgc ggtgagtttg aacagatccg
     6601 ccggagtgtg gacatattct ttttcaacca gctgatcgat gattttgtcg cccattccgt
     6661 caacatccat cgcacggcgg gaaacaaagt gtttcagcga ctctttacgc tgcgcaccgc
     6721 aaatcaggcc accggtacag cgggcaaccg cttcaccttc cacacgctca acgtcagaac
     6781 cacataccgg acaatgcgtc gggaatacaa cctcacgggt atcttccggg cgttcagaaa
     6841 gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg gcgaatcacc actttatcgc
     6901 caatgcgtaa accaagacgt tcgatttcat ccgcattgtg taaggttgcg ttactcacca
     6961 gcacgcctgc aacatggaca ggttccagac gcgcaacagg cgtaatcgcg ccagtacgcc
     7021 caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc ctgcgccggg aatttaaacg
     7081 ctaccgccca gcgcggggca cgcgcgacaa agccaagctg ctcctgctgt gccagtgagt
     7141 tgaccttaat caccacgccg tcgatatcaa agcccagcgt cgggcggtct tcttccactt
     7201 tgtgatagaa cgccagcact tcttccgccg attcacaaag cgttacccga tcgctgaccg
     7261 gcaaccccca ctgtttaaat tgcagtaaac ggccaagatg agtatccggc agctcgccac
     7321 cttccagaac accaacgcca tagcagaaaa aagtgagcgg tcgcttcgct gtaatacgcg
     7381 gatcaagctg acgcagtgaa ccagctgccg cattacgtgg gttagcaaaa actttcccgc
     7441 ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc ctgcggcagg aacacttcac
     7501 cacgcacttc cagacgcgcc gggatattct ctccgtgtag cttcagtgga atggcgcgaa
     7561 tggtacgcac gttagacgtg atatcttccc cggtggtgcc atcgccacgg gtcgcggcac
     7621 tgactaaaac gccattttca tacagaatac tgacagcaag gccatccagc ttcagctcac
     7681 agcaccaggt gactttctcg ttgcttttca gacggtcctg cactcgtttg ttgaaggcaa
     7741 gaaagctttc ttcatcaaaa acgttatcca gcgacagcat tggcacttca tggcgaatct
     7801 ggctgaaagc cgccagcggc gcagcaccta cacgttgggt aggcgaatca ggcgtaatca
     7861 gttctggatg tttggtttcc agctcgcgca gttcgcgcat cagcctgtcg tattcagcgt
     7921 cgggaatttc cggcgcatcc atcacatgat aaagatattc atgatggcga agcgtcgttc
     7981 gcagttctgt cagttgttgt tcgattgatt ccatatcgca ccatcaatgc taaaaacccc
     8041 cgacaagcgg gggttcgaag aggagttaat ttgccttaag tgtatcaggc gttggcgtct
     8101 ttgacttcgc ggatgatgtc ctggtactca cgcaatttct gcggagtcat catacggcgc
     8161 tgatcgtcaa gcacgacacc gcccacttca tcggcaatat gctgcgcaga ttgcagcatc
     8221 agcttgaagt tctgcagctc gtcaccgtaa gacggtacct gcataaagat agtgacaccc
     8281 ggagtagtga aatccttcat ttcaggatca aaggttcccg gtttcaccat attcgccagg
     8341 ctgaataacg ccgggccgct gccatccggg ctaagatgac gatggtaaat attcatatcg
     8401 ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca gttcaccgtt tagctcgcta
     8461 ccatgatgcg ccgcgacgtt catgataatc accgcttctt tgcgcttcgg tttatccata
     8521 actggagccg gttccgctac aggctcaggc tgtggtgccg ctacgggttc tgcaggctgg
     8581 aaagcctgtt gtgccggttg cggtgctgaa tgcactggct gcggcgctgg cgcgacctgt
     8641 ggcgaaactg gctgctgcaa cggctgttca ggctgcggct gataggcagg ctgctgcacg
     8701 ggttgctgca ccggctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
     8761 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggttggta ctggtgttgc
     8821 ggcgacggac gagcagcctc atgctcctgg gcgttagccg gggcatgatt cacgcggtga
     8881 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
     8941 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
     9001 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
     9061 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
     9121 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
     9181 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
     9241 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
     9301 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
     9361 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
     9421 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacgca actcgatgtc
     9481 tggatcccga ctctcatgag ttatgttccg gactggttac aatggctgag ctatctgttg
     9541 tggcccctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc aacgattgct
     9601 aactggattg ccgctccgtt caacggttta ttggctgaac aactggaagc acgattgact
     9661 ggcgctacac cgccagatac cgggattttc ggtatcatga aagatgtgcc gcgaatcatg
     9721 aaacgcgaat ggcaaaaatt tgcctggtat ctgccgcgcg caattgtatt actaattctt
     9781 tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
     9841 tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa
     9901 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
     9961 agcctgttta cgatgatccc gctgcttaat ctgttcatta tgcccgttgc cgtttgtggc
    10021 gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct
    10081 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccga cccttattcc
    10141 atcttgcatg tcattatttc ccttctgtat atagatatgc taaatcctta cttccgcata
    10201 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
    10261 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
    10321 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    10381 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    10441 aggcgttgaa ctggttgaac cgaccagcgg taataccggg attgcactgg cctatgtcgc
    10501 tgccgctcgc ggttacaaac tcaccctgac catgcccgaa accatgagta ttgaacgccg
    10561 caagctgctg aaagcgttag gtgcaaacct ggtgctgacc gaaggtgcta aaggcatgaa
    10621 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    10681 gcaacaattc agcaatccgg caaaccctga aattcacgaa aaaaccaccg gtccggagat
    10741 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    10801 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
    10861 cgccgttgag ccaaccgatt ctccagttat cgcccaggcg ctggcaggtg aagagattaa
    10921 acctggcccg cataaaattc agggtattgg cgctggtttt atcccggcta acctcgatct
    10981 caagctggtc gataaagtca ttggcatcac caatgaagaa gccatttcta ccgcccgtcg
    11041 tctaatggaa gaagaaggta ttcttgcagg tatctcttct ggagcagctg ttgccgcggc
    11101 gttgaaacta caagaagatg aaagctttac caacaagaat attgtggtta ttctaccatc
    11161 atcgggtgag cgttatttaa gcaccgcatt gtttgccgat ctcttcactg agaaagaatt
    11221 gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc accttttcag gtgctttttt
    11281 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    11341 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    11401 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    11461 acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg cgtcgcgtct
    11521 gttaaaaact ggcgctaaca atacaggcta aagtcgagcc gccaggctag actttagttc
    11581 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    11641 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    11701 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    11761 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
    11821 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    11881 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    11941 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga
    12001 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    12061 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    12121 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    12181 tcatagccct gattaaagat aaacacatga cagctgacgc agctgctcat gaagttatcg
    12241 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    12301 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    12361 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg
    12421 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    12481 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    12541 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    12601 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    12661 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
    12721 tagaagtgtg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    12781 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca
    12841 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaagcgg
    12901 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    12961 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga
    13021 tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta
    13081 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaattt
    13141 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    13201 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    13261 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    13321 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    13381 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    13441 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    13501 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    13561 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    13621 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    13681 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaagaa ggataccgga
    13741 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    13801 gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta tcaaaccaac gggtaacaaa
    13861 atggtcgcgc cagtagacgg caccattggt aaaatctttg aaaccaacca cgcattctct
    13921 atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg
    13981 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact
    14041 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    14101 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgtg
    14161 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    14221 atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc
    14281 tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg ctcggtgcac tgcatcggtt
    14341 aacgccttcc ctttcagcaa gccactgatg agctgagcac aaaacaggtc gccagtccct
    14401 ttcaggtcgg tttttacccg tgaatgggaa atgacattca cgctgtcggc agtgaccact
    14461 acaacctgca tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt
    14521 aatgtgtctg aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt
    14581 ttaccggtca ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt
    14641 aaatattgtc gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg
    14701 ccaatcaccg gatcgaccat gatcaataga tcaggatggt ctttgcgtag cgcagtcagc
    14761 cactcggcaa ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagcacga
    14821 agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcgtcc
    14881 ggaatcgcac caccgtagaa agtgtcataa tgcggcgtat tgctcagcaa taccgtcggc
    14941 acggcaaaga cattcaggcc gttctgtttg atagcaggca cggcaatgct gttgcctacg
    15001 ctgccgtaca ctacctgcga ctgcacggcg acgatatccg cctgcagtgc cctgctctta
    15061 tcgttaaaca acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt
    15121 attccaaccg aaactttacc tgattctggc agtcaaatcg gctattacaa aacaaggata
    15181 aggtaattca gtgaagaaaa tcatttgtct ggtcattaca ctactaatga cactccccgc
    15241 ttacgcgaag ttaactgccc atgaagaagc ccgcatcaac gccatgctgg agggattagc
    15301 acagaaaaag gatttgatat ttgtgcgcaa cggttatgaa catacctgcg atgaagcggt
    15361 ttctcatctg cgtctgaagc tcggcaatac ccgtaaccgc attaacactg ccgagcagtt
    15421 tattgataag gttgcttcgt catcatcgat taccgggaag ccgtatatcg tgaagatgcc
    15481 cggtaagagc gatgagaatg cacagccttt tttacatgcg ttaattgcgc agacggataa
    15541 aacggtgcct gcgcaataat cttaaatccc cgccccctga ctaaaatgct cttccccaaa
    15601 caccccggtg gaaaggtagc gatcgccacg atcgcagatg atcgctacca ccaccgcacc
    15661 agggttagct tttgccaccc gcagtgctcc agcaaccgcg ccgccggagc tgacgccaca
    15721 gaaaatccct tcccgcactg ccagttcgcg catggtgttt tccgcatcgc gctgatgaat
    15781 atccagcacc tcatccacca gagaagcgtt gaaaatcccc ggcagatatt ccgcaggcca
    15841 gcggcgaatg ccgggaatgc tactgccctc ttccggctgc aggccaacaa tggtcaccgg
    15901 tttggattgt tcgcgcataa agcgtgacac gccggtgata gtgccggttg ttcccatgct
    15961 ggagacaaaa tgggtaatgc gcccgccagt ttgctgccag atttccggcc cggtagtggt
    16021 gtaatgggcg taagggttat cgggattatt gaactgatcg agcagctttc cttcaccacg
    16081 attagccatc tccagcgcca gatcgcgcgc accttccatg ccctgctctt tggtgacaag
    16141 aatcagttcc gcaccataag cacgcatcgc cgcacggcgt tcctggctca tgttgtcggg
    16201 catcagcaat ttcatgcgat agcctttaag cgcggcaatc attgccagcg caatgccggt
    16261 gttaccactg gtggcttcga ttaacacatc acccggttta atttccccgc gcttttccgc
    16321 ctcaacaatc atcgatagcg cggcacgatc tttcaccgaa ccggccgggt tattgccttc
    16381 cagctttaac cacacttcac tgccgttatt cggccccatt cgctgcaact tcaccagagg
    16441 cgtattgcct attgtttgtt ctaatgtact cacgatctct gtccatacgt ggtgtcgcct
    16501 gatgcgacgc ttgcgcgtct tatcaggcct acaggttaca aaccttgcca ttaaaaagcc
    16561 cggatcgcgg gaagcgcctc cgggcgttta acattcactc aacctatcag gcgctttgtg
    16621 cgagagcaag ttcctcatct cgggtttcga tacgctcgtc gccgttatac agccgcgcat
    16681 gttgcagacc aacgaataaa cgctcgccac gctgcggggc atcgtcgcca tgcatcacga
    16741 ccgtcagcgg ttcgttgtac caccccagcg gctgcaccac taattgggtg tagtgacctt
    16801 tcgggctggc ttccagtacc tgtaccggca gcggcgaatc gaggctggta cggcggctga
    16861 tatccacttc ccaagggcgc aggaagagat ccaccggccc ctgatacgca ggtgtgtagc
    16921 ccagcggcca gcgatgcgcg ccaacatgga actgcccgcc gcgaatggtt ccctgcaggc
    16981 ggttcacttc gcccataaat tcgagcacaa aacgggtcgc cggttcgcgc catacctgat
    17041 ccggcgcgtc agcctgttca atattgccct ggctcatcac aactacacga tcagctactt
    17101 cggtcgcttc ttcctgatcg tgggtcacaa aaacgctggt gaattttagt tcttcatgga
    17161 gttgacgcag ccagcgacgc agctctttac gcacctgcgc atccagcgcg ccaaacggtt
    17221 catcaagcag cagaatttgc ggttccacgg caagcgcgcg tgccagcgcc acacgctgtt
    17281 tctggccgcc ggaaagctgc gccggataac gatccgccag atgagcaagc tgcaccatct
    17341 ccagcaattt tgtcactttc gctttgatcg ccgcggcatt cgggcgctcg cgacgcggca
    17401 gcaccgtcag gccaaaagcg atattgtcga ataccgtcat atggcggaac agcgcgtaat
    17461 gctggaacac gaaaccgact ttacgatcac gtgcgtgcag gcgactcacg tcggtgccgt
    17521 ggaagcgaat atgcccgctg gtttgatgct ccagcccggc gataatgcgc agcagcgtgg
    17581 ttttcccgga accggacggc cccagcaacg cgaccatctg acctgaagga atatccagtg
    17641 agatatcgtt cagcacctgg gtgcgaccaa acgacttctt aatattggca atctcaatgc
    17701 tcatgatttt cctcctgctg cgcgcgtttt tcctgattct ccaggcgcca ctgcaacata
    17761 ctctttaaaa acagggtgat aatcgccatc agcgtcaaca gcgcggcggc ggtaaaggag
    17821 ccgactgtgt tgtagtcctg ctccagcaat tcaatctgta acggtagcga aagtgtttcg
    17881 ccacgaatcg agccggaaac caccgacacc gcgccaaact cgccaatcgc gcgggcgttg
    17941 gtcagcacta cgccataaag cagcgcccag cggatgttcg gcaatgtgac gcggcggaac
    18001 atctgccagc cggatgcgcc aagcaaaatc gccgcttcgt cttcctgact gccctggctt
    18061 aacatcaccg gcaccagttc gcgcaccaca aacggacacg tcacgaagat cgtgaccagc
    18121 accattccgg gccaggagaa cataatttgc agattatgtt catccagcca gccgccgagc
    18181 gggccgttag agccgtagaa cagcagatac accagaccgg caaccaccgg cgatacggca
    18241 aacggaatgt ccagtagcgt cagcagtaac tggcgtccgg ggaagttaaa gcgcgtcacc
    18301 agccaggcca gcagaatgcc gaacaccagg tttaccggta cggcaatcag cgcaatcatc
    18361 accgtcagcc aaatcgcgtg caacatgtcc ggatcggcca gattctgtaa aaccggcatc
    18421 agccccttgc tgaatgcctg cacgaagatg taaatcatcg gcaccagcag gatgaacgcc
    18481 gaaaccagca tcccgatgcc aattagaaac catttgcccc agttaatcgg gcgcgcgtca
    18541 taacgcttca attgtgtcac ttccgccatt aatgacctac cacacgccga ccaaagcgac
    18601 tttgcagagt gttaattgag aacagcagca gcagagatgc cgcgaggatc accgaagcaa
    18661 tcgcgctcgc cgccgggtaa tcaaactcct gcaagcgcac aaaaatcatc agcgacgtca
    18721 cttccgtctt ccacgcgata tttccggcga taaaaatcac cgcgccaaat tcaccaagac
    18781 tacgggtaaa cgacagcgcc acgcccgcca ccagcgccgg agaaagctcc ggcagcacca
    18841 ctttgcagaa actctgccag cgcgttgcag caagcgtttc tgccgcttct tcatattccg
    18901 ggcctaactc ttccagcacc ggctgcacgg tacgtaccac aaacggaatg ctggtaaagg
    18961 ccattgccac cgcaatcccc agccaggtat aggtgacttt gatatcaaac ttcgccagcc
    19021 attcaccgta aaaaccgttt acagaaaaga gcgaggccag cgttaaaccg gcgactgccg
    19081 ttggcagcgc aaagggtaaa tccatcagcg catcaagcag cgtgcggccc ggaaagcgat
    19141 aacgggttaa aatccacgcc atcagcaggc cgaaaacgcc gttaaaaatt gatgccacaa
    19201 acgccgacag cagcgtcact ttgtaggccg cgaccacctg cgggttggtg atcacttccc
    19261 agtactgcgc ccagctcatc tcagagagct gcatcaccaa cgcggagagc ggcagcagca
    19321 aaatcaggca cacaaacagc agactggtgc cgaggcttaa ggtaaagccc ggcagcacgc
    19381 gtcttgatga aactgcaaac attaattacg ccccgccgct aacagcttgt ctaactcgcc
    19441 gccgctggtg aagtgggttt tcatcacttc cggccaggaa ccaaatttgt cttccacgcg
    19501 gaacagctcg gtctgcggga atttgtcttt cagtttgtcc atgacttccg ggttattcac
    19561 gcggtagtaa tagtcggtga tgatggtttg cgcctgcggg ctgtagagcc agttcaggta
    19621 ggctttggcg gctttttccg taccgttggc ctgcacgttt ttatcaaccc acgccaccgg
    19681 gaattccgcc agaatgttgg ttttcggaat caccacttca aaaccctgcg cttcatactg
    19741 tttacggatg ttgttcactt ccgactcgaa gctgatcagc acatcaccca ggccgcgctc
    19801 ggcaaaagtg gtggtcgcgc cacgaccgcc ggtatcgaac acttcaacgt ttttcaggaa
    19861 ctgagtcata aactgttcgg ttttggcttt atcgccaccg tcagctttat ccgctgcgcc
    19921 ccacgcggct aaataggtgt aacgcgcgtt acccgacgtt ttcgggttcg ggaaaatcag
    19981 cttcacgtcg gagcgcacca gatcgttcca gtcgtggata ttcttcgggt tgcccttacg
    20041 gaccaggaag cccatggtgg agtagaacgg cgagctgtta ttcggcaggc gcgactgcca
    20101 gtccgccggg ataagcttgc ctttatcgtg cagaatttgt acgtcggtca cctggttata
    20161 agtgacaacg tcggctttta agccctgcaa aattgccagc gcctgttttg atgacccggc
    20221 atgagattgt tttatcgtca gtttgtcgcc gccgttatct ttggcccatt gctgttcaaa
    20281 cggcgggttc aaggcggcaa acagctcgcg ggagacgtca taagaactgt tcagcagttc
    20341 cgttgcctgt acatggcccg ccagcagcag agaagcgacc agcgcgagtg agttcttttt
    20401 cagtaagtta acggccattg cgcaccctta taaatttaat gactttctaa tagccatcat
    20461 atttataacg gacgtgaaag gagtaacggt tttatatacc gtttggtgat ttggaagttg
    20521 aaaagggaat aagacgaaat cctccccaca catgcaggga ggaaacagag gtgaatcaga
    20581 taccgacgct aaccgtctct ggcagtgtgc tgccgccatc aatcacattc tgtgtaccgg
    20641 ttaaatagct ggattcatcc gatgccagga aggccgccag ttcgccgact tccagcggat
    20701 cggcgaggcg acgcagcggg attgcttttg ccatatcagt cagcaccgac tctggatctt
    20761 ccgggttcga ctggcgggca atgctttccg ccattggcgt gcggacgtat cctgggcaga
    20821 tggcgttaac gcgaataccg gattgcgcgt actccaccgc cagcgatttt gtcaggccaa
    20881 caatcgccgc tttcgttaag gcgtacgccg tttcacccgg atcggccacc atatcaccag
    20941 tgactgaaga catcatcaca atgcgaccat ctttgcgggc aatcatctcc ggcagcaccg
    21001 ccttcgtgac gttccatacg cctttaatat tgatgtcaat atggaaatcg cgatcgtcat
    21061 cgctcatatc gaggaaactg cccagacgac aaacgcctgc gttattcacc aggatatcaa
    21121 tgcgcccttc tttttccttc gcgcgtttga tagctgcggc taccgacgcc gggtcacgca
    21181 catcggcgac aaccgccgta cagcgatgac cacgaccaca cagttcgtcc gccagctttt
    21241 cgatctcagg ggagatatcc agcaagatta ggtttgcgcc atgacgcgca aaagttctgg
    21301 caattccttc gccaattccc tgcaatgcgc ccgtaatcag tgctgtcttg cccgtgagtt
    21361 tacccatttt aaagctctcc ttgttaatac agagcgttac actccattcc ctctgaaaat
    21421 acaggataaa caaaatattg ctgcggatca gctcactaat tcaggaaaat ctcaatccag
    21481 tcgctgcttt atgcatcatt tcaggcgttg tgttagctca ctgctgcgct gaatcatttt
    21541 caatgactcc atatcattga gttgcacaag accaacaaac agtaaatcgg taacagaatt
    21601 ttgcgccgtg cgggtagaca tcgacgagct acgccattcg gtttcaccgg aaacggtatc
    21661 gagcgtgaaa tgcgccagcc gccgcagcgg agagtcagcc agagaagtaa tggcaatcac
    21721 cgttgccccc tgctttcgtg ccgcttctgc gcacaagaca atctcttttt tactgccgct
    21781 gtaagagata gcgatttgta catcgccttt tttcaacgcc tgggaaacgg tagcttgtac
    21841 gtgcgtgtcg gcttcgcagg ccacgcgata gccaattttc atcagtttga acgacagatc
    21901 gcgccccacc agcgccgatc cgcccaggcc cgttatctgg ataaacggtg ctttactgat
    21961 gacctcaatg attttttgca gccgcgcgta gtcgaacaac gcacaggttt gctccagcgc
    22021 cagctctttt tcgcgattca atttgcgggc aatcacttcc agcgaatcat cactggtaat
    22081 cgaactgtgc aggtgcagcg ccgtggcatt cgttttctcc cggctggcac tgtattcgcc
    22141 aattaatgcc atacgcaatt cagtaaatcc ttgcgcgccg agcttctggg caaatttcac
    22201 aatactcgac tggctgatgc cgagctgttt cgccatcttg cgcgaagaga cggatttcag
    22261 ctcactcacg ttggcccgca gaaagtcagc aattttttgc tcgttctcgg taaattcact
    22321 ttccgcattg cgtattttcg tcaggtacaa catcttccac tctcctgggc tggcagcaaa
    22381 aattccgctt aagttcttat gacgctcttc acactctgcg agtgtagact caatgattca
    22441 tttaagtctg tcaattaggg atatatgatt ccatacatct gcaattattt gatcgttaat
    22501 agtaaggtca ccaccgatgc aacttgaaaa gatgattacc gaaggctcga acgccgcctc
    22561 ggctgaaatt gaccgcgtat caacgctgga aatgtgccgg attatcaacg atgaagataa
    22621 aaccgtaccg cttgccgttg agcgcgtact gccggatatc gccgcggcga tcgatgttat
    22681 ccacgcccag gtaagcggcg gcggtcgtct gatttatctc ggtgcgggaa catccggtcg
    22741 tctggggatt ctggatgcca gcgaatgtcc gcccacctac ggcgtgaaac cgggtttggt
    22801 ggttggtttg attgctggag gcgaatatgc cattcagcac gcggtggaag gcgctgaaga
    22861 tagccgggaa ggtggtgtta acgatctgaa aaatattaat ttaacggcac aggatgtggt
    22921 ggttggtatt gcggccagtg gtcgcacgcc gtatgtaatt gccggactgg aatacgcacg
    22981 ccagctcggc tgccgcacag tgggaatttc ctgtaatccg gggagcgccg tttcaaccac
    23041 cgcagagttt gccattacac cgattgtagg tgccgaagtg gttaccggtt cttcgcggat
    23101 gaaagcgggt acggcgcaga aactggtgct caatatgctc tccaccgggc tgatgattaa
    23161 atccggcaaa gtgttcggca acctgatggt cgatgtggtc gccaccaacg aaaaactgca
    23221 tgtgcgccag gtcaatatcg ttaaaaacgc caccggatgt aatgccgaac aagcggaagc
    23281 ggcgttaatt gcctgcgagc gcaactgtaa aacggccatt gtgatggtgc tgaaaaatct
    23341 cgatgccgca gaagctaaaa aacgcctgga tcaacacggc ggctttattc gtcaggtttt
    23401 agacaaggaa taacccatgg ccaaagagat cagcagtgaa cttctgaaca ccattcttac
    23461 ccgtgtcggc ggaccgggaa atatcgccag ttgtggtaac tgtatgacgc gcctgcgtct
    23521 gggtgtacat gacagttcac tggttgatcc caatatcaaa acgctggaag gcgtgaaggg
    23581 cgtcattttg accagcgacc aggtgcaggt cgtttttggg cctggtaaag cgcatcgtgc
    23641 cgcaaaagcg atgagcgagc tgctgggaga agcaccggta caggatgccg cagaaatcgc
    23701 cgcccagaac aaacgtcagt taaaagccaa acaaacctcc ggcgtgcaac aatttctcgc
    23761 caaattcgcc accatcttca cgccgcttat ccccggtttt attgccgccg gtctgctgct
    23821 gggaatagcg acgttaattg ccacggtgat gcacgttccg gcagatgctc agggcacgct
    23881 acccgacgcg ctgaatttta tgaaggtgtt cagcaaaggt ttgttcacct tcctggtgat
    23941 tctggtgggc tataacgccg cccaggcatt tggcggcacg ggcgtcaatg gcgcaattat
    24001 tgccgcactc tttttgctcg gttataaccc cgctgcaacc accggttact acgccggttt
    24061 tcacgatttc tttggtctgc ccatcgatcc gcgcggcaat attatcggcg tgttgattgc
    24121 cgcgtgggcc tgcgcccgca tcgaaggcat ggtgcgccgc tttatgcccg acgatctcga
    24181 catgctgctg acctcgttaa tcaccctact gatcaccgct acgctggcct acctgatcat
    24241 tatgccgctg ggcggctggt tattcgaagg tatgtcgtgg ctgtttatgc acctgaacag
    24301 taatccgctc ggttgtgcgg ttttagccgg gctgttcctg atcgccgtgg tatttggcgt
    24361 gcatcagggc tttattcctg tttacctcgc gttaatggac agccagggat tcaacagctt
    24421 atttcccatc ctttcgatgg caggcgcggg ccaggtgggc gcggcgctgg cactctactg
    24481 gcgggcgcaa ccgcacagtg cattacgcag tcaggtacgc ggggcgatta ttcccggcct
    24541 gctgggcgtt ggcgaaccgc tgatttatgg cgtcaccctg ccccgcatga aaccgtttat
    24601 taccgcttgt ttaggcggcg cggcgggcgg tttgtttatc ggcctgatag cctggtgggg
    24661 tctgccgatg ggcttaaaca gtgctttcgg gccatccggc ctggtggcgc tgccgctgat
    24721 gacttccgcg caaggcattc ttccggcaat ggcggtttat gccggtggga ttctggtggc
    24781 atgggtttgc gggtttattt tcaccacgct ctttggctgc cgtaacgtca atctggactg
    24841 atatcatgaa acggacaatg ctctatcttt ctctgctggc tgttagttgt agcgtcagcg
    24901 ccgcaaaata ccctgttctg acagaaagtt cgccagagaa agcagggttc aacgtcgaac
    24961 ggcttaacca gatggatcgc tggattagcc agcaagtgga cgccggttat cccggcataa
    25021 acctgctgat tataaaagat aatcagattg tgtatcgcaa agcctgggga gcggcgaaaa
    25081 agtacgatgg cagcgtattg atggaacagc ctgtcaaagc caccaccggg acgctgtatg
    25141 acctggcttc caataccaag atgtacgcca ctaactttgc tctgcaaaag ctaatgagcg
    25201 aaggcaagct gcatcctgac gatctgattg cgaagtatat tccagggttt gccgatagcc
    25261 cgagcgatac gattaaaggc aaaaacacgc tgcggatcgc cgacctgctg catcacagcg
    25321 gcggtttccc ggcggaccca caatatccga ataaagcggt cgcgggcgcg ttatattccc
    25381 aggataaagg ccagacgctg gagatgatca agcgcacgcc gctagagtat cagcccggca
    25441 gcaagcatat ttacagtgat gtcgattata tgctgcttgg atttatcgtc gagtcggtca
    25501 ccggtcagcc gcttgaccgc tatgttgaag agtcgattta tcgcccgctc ggcctgacac
    25561 atacggtatt taacccgctg ctgaaaggct ttaaaccgca gcaaattgcc gcgactgaac
    25621 tgaacggcaa tacccgcgat ggagtgatcc acttcccgaa tatccgcacc tccactctct
    25681 ggggtcaggt gcacgatgaa aaagcctttt attcgatggg cggcgtttcc gggcacgcag
    25741 gtttgttttc caataccggc gatattgcgg tgttaatgca aacgatgctg aacggcgggg
    25801 gctatggcga tgtgcagctg ttcagtgcgg aaacggtgaa gatgttcact accagctcta
    25861 aggaagacgc cacttttggc ctcggctggc gcgtgaatgg taatgccacc atgacgccga
    25921 cgtttggcac gctggcaagc ccgcaaacct acggtcacac tggctggacc ggaacggtga
    25981 ccgttatcga tccggtgaat catatggcga ttgtgatgtt aagcaacaag ccacattcgc
    26041 cggttgccga tccgcaaaag aatcccaata tgttcgaaag cggtcagctg ccgattgcaa
    26101 cgtatggttg ggtagttgat caggtgtatg cggcgttaaa gcaaaagtaa taaaaaggcc
    26161 agtcagtaat gttactgcct ggcctttaat caatgaatca gaaacgctta cagcgccatc
    26221 aacttgtcca gcgacggtgc gaaataatag ccgccggtta ccggtttggt gaaacgcaac
    26281 atcgcatcac gcttaccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta
    26341 tgcagacgcg cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac
    26401 ggcaggctct ggcgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtg
    26461 aggtgagagg tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc
    26521 ccgatcatca tctcctgatc gtgaacgctc atccggttga gctgcttcag gttgtgctcc
    26581 caacgctgca caaacacata gctgccgccc gcatccacgc cgtctttgat aaccgccact
    26641 tcgcgacgcg tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca
    26701 cgctcttcca cccaacggaa gccgtggatc tcttctttca cttcaatgca gtcaccaaag
    26761 gcttccatcg ccgcctgggc gacagagaag tttacgtcgt gacgcagaga aagaatgtgg
    26821 atcaacacat cgaactgggt ggtcggcgca aggcctttac cgtagcccgg aaaatctttc
    26881 agctcttcag ccccaacgcc gccgctcagt gcgcgccagg tgttgttacc aaaggcaacc
    26941 accgcaccaa gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat
    27001 gttttactgg ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc
    27061 gccgcgcggc aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa
    27121 aataagaatg ccacgcaatc cgtggcattg ttgggcgcta ttttacgtgt tttttcgcgg
    27181 cggcggtttg ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt
    27241 tcagggtatc gtccgaaggc attaaccctt caggaccact ccattcaccg ctgaactggt
    27301 agctaatatg ttgactgcct tcggctttac attcgacgac acgattatca tcaccatcgg
    27361 ctttttggca attgccaaat gctttgctgt aaaggtcgct aaacggtgta ccgattttaa
    27421 caccggtgtc agcaggaata tcgctatcca gcacatcaat gcggctgatc gtgccctgat
    27481 cgccgttaat caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcactacat
    27541 taccgttagc ggttttcatc ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa
    27601 tggcttgttc ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt
    27661 tggtggacga accaaaccag ttccacgggt tagcagcaga ccagttaact gacgacatcg
    27721 tggaacagcc ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt
    27781 cacttccttt ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt
    27841 attcttttac ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg
    27901 ccacaatatc cgccactacc agcgccagac cgaaaccgct aacggattca ccgttcagcc
    27961 acagccacgg ttgccagcaa agtaaaacca cctgcgccaa cagcaacaga aaatagagta
    28021 cacgccaggt gcgaggaaac gtagcccgcc gaccgctcaa caaaaacgtc agcaccgccg
    28081 gaatgccagg aatcagcccc agccagaaat tatcgtgatc gggataaaac agatttagca
    28141 gcgcagtacc ctgctcgcgc gacgcaccgg caatgacaaa cagcacccag gttcgcgcct
    28201 gaagcaatag cacaagccag aagagcaagg gtaaacgcag gcgaccgtgc gcatcataat
    28261 ggacaggatg aaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg
    28321 tcggcgtgtt catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc
    28381 acgttgatct gaattttcgg gcagccgcga gcaatcagct ttttctccag ccggttaagc
    28441 aacgcattgg caattccacg cccacgaaac tctggatgca cgccaagata ataagcagac
    28501 ccgcgatgcc cgtcataacc gcccatcacc gttccgacca cttcaccgtt tacctcagcg
    28561 accagaaaca aactgacgtc atggttcatc ttacgctcga tgtccatttc cggatcgttc
    28621 cacggacgca gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg
    28681 cgaaatacgc gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg
    28741 gcgtgaacgg tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg
    28801 ggagttgtca actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg
    28861 gattacgcga tacgatataa catctggaac tttattatta caactcaggc cgtatgagca
    28921 cttttaaacc actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg
    28981 ccctgacgtt gtcaggaatg tcgcaagcca tcgccaaaga cgaaccttta aaaaccagca
    29041 acggacacag caagccgaaa gccaaaaaat ctggcggcaa acgtgtcgtt gttctcgatc
    29101 caggccacgg cggaattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg
    29161 tggtgctggc gattgctaaa aacgtccgtt ccattttgcg taatcatggg attgatgcgc
    29221 gtttaacgcg ttctggcgat acgtttatcc cactttacga tcgcgttgaa atcgcccata
    29281 aacatggcgc agatctgttt atgtcaattc atgccgatgg ctttaccaac ccgaaagctg
    29341 ccggtgcttc ggtatttgcc ctctctaacc gtggggcaag tagcgcaatg gcgaaatacc
    29401 tgtctgaacg cgaaaaccgc gccgatgaag ttgccggtaa aaaggcgact gacaaggatc
    29461 acctattgca gcaagtgctg tttgatctgg tgcaaaccga tactattaaa aacagcctga
    29521 cgctcggctc gcatattctg aaaaagatta agccggtgca taaactgcac agccgcaaca
    29581 ccgaacaagc cgcgtttgtg gtgttgaaat cgccgtcggt tccttcggtg ctggtggaaa
    29641 cctcgtttat caccaacccg gaagaagagc gactgttagg cacggcggcg tttcgtcaga
    29701 aaatcgccac agcgattgct gaaggcgtga tcagttattt ccactggttc gacaaccaga
    29761 aagcacattc gaaaaagcga taagttatga aacccgacgc acaccaggtt aaacagtttc
    29821 tgctcaacct tcaggatacg atttgtcagc agctgaccgc cgtcgatggc gcagaatttg
    29881 tcgaagatag ctggcagcgc gaaggtggcg gcggcgggcg tagtcgggtg ttgcgtaatg
    29941 gtggtgtttt cgaacaggca ggcgtcaact tttctcacgt ccacggcgag gcgatgcctg
    30001 cttccgccac cgctcatcgc ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt
    30061 cactggtagt gcatccgcat aacccgtatg ttcccaccag ccacgcgaat gtgcgttttt
    30121 ttattgccga aaaaccgggt gcagagcccg tctggtggtt tggcggcggc ttcgacttaa
    30181 cccctttcta tggtttcgaa gaagatgcca ttcactggca ccgcaccgcc cgcgacctgt
    30241 gccaaccgtt tggcgaagat gtttatccac gctacaaaaa gtggtgcgac gattatttct
    30301 acctcaaaca tcgcaacgaa cagcgcggca ttggcggact gttctttgat gatctgaaca
    30361 cgccagactt cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg
    30421 cttatttacc aattgtagag cgacgtaaag cgatggccta cggcgagcgc gagcgcaatt
    30481 ttcagctcta ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcactc
    30541 tgtttggcct gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg
    30601 tacgctggga gtatgattat cagccaaaag atggcagccc agaagcggcg ttaagtgagt
    30661 ttattaaggt cagggattgg gtgtaactcc ctcaccccca ttcccgcatc cgctgatgca
    30721 gcgtcaacga cggcttctcg gcaaacagct gctggtaatc cgtggcaaat tgccccagat
    30781 gccagaatcc ccactgcatg gcggcgtctt ttaccgttgt gctttgcgac cacgggctta
    30841 tcagttcgcg gcgcacggcg ttcaggcgaa tgcgtttcag ccacgcgttt gggccaatgc
    30901 ctaaaatagc gtgaaacgcg ttttgtagcg tgcggcggct gacatgcagt tgattacaca
    30961 aatccagcac cgtcaccggt tcggacatgt tttccagcac atattcacgg gcgcgggaaa
    31021 gtaatctacg gtaactctga tgactgatgc tttccgccgt caccattggt tgcgcgtctt
    31081 cgagcatcgc ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct
    31141 gatggagatt ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca
    31201 gcgcggcttt atgctgctct ttcacttcca gcgccgactg gctacgcagc atatgtaata
    31261 cccgatccgg attgtgcaaa aagttagcct gacgggtgat gacatcttca gaaagcacta
    31321 cgcccaggat cgtgtaatcg tccggcgtac ttaattcaaa ctcggttcca ccggggcgcg
    31381 tcgctatttc tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg
    31441 ccggaatgcc aaaccagaac gagttaggcc agaccaggca cgactggcgc agcgccagac
    31501 cggtgtattc acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa
    31561 acttgcccgg atgcagctga tcgtaaatct gctgccaggc ggtaatcgtt aaagcatgtt
    31621 catagacatc cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct
    31681 tcaggttttc gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt
    31741 tcatgatgtt aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct
    31801 tgagacgata acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg
    31861 catttctggc tttttccagc gaccagccaa agtgggcagc cacttctcct gccgtcattc
    31921 cctgacgtac tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg
    31981 gcgcgtcggg ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact
    32041 gggtgtcatc gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg
    32101 cggcagcaca acctgcgtcc agcgcagcac gacacgccgc cagatcgcct tccaccacca
    32161 gcgttaagcg accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga
    32221 gcatggcatc cgctgcatct atcgcagcga ccatgccgtc cacttccagc aatcccagtg
    32281 cattgatcat cggcaacctc cattacgcac gctggattgg attacgcgcg atttccagca
    32341 ctgcatcggt aaaggcgtta caggccgctt tacacgctgc ctggctaccg gttaaaaatg
    32401 ctgccgagta gttagtttca gacggtggcg ggacataggt caccagctgt acgtcggcag
    32461 atttcaacgc tgcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca
    32521 tcggatcgcc aagcgtgatc ccggcggttg atgagagata agaaccggta cgcgaaacta
    32581 catgtgccag gaatgccgta tcttgcgcat cattagccca ctggaaagca gcgccatttt
    32641 caatatgcgc aaccatcgca tccagacctg cacgcacttc cgccgggttt ggcccaccaa
    32701 gcataatcag cacttcaccg gccgtcggtg acgggccgtg tgcagcgccc gcatacagcg
    32761 agcggccata caccacttcc accatcgcct gcttggtcgc ttcatcagcg gcaatgtagg
    32821 tgacgtcatc ggagtcggca gaaatcagac cgaggctacg aatatgcggc ggcaatttta
    32881 gttcacgcgc aaaatcggcg ttaacggagg caatcacccg catagcggtg acagacggtc
    32941 gaatcaaatc taaagctggc atgatgcctc cttatcgggt catgttgatg ccggatgctt
    33001 tctgctccag catacgtttc gccaaatcca caatgacggc tgcggcttca accggcggcg
    33061 tgccgccctg gtgaatgtta gagatacagg tgcgatcggc ctcaacggtg gtcgccatac
    33121 gtggcgagta aacggcgtag caggagaggc tttctgactg acccaacccc ggacgttcgc
    33181 ccaccagcag gatcaccact ttcgcgccga ggatctcacc gatctgatct tcaatcttca
    33241 cgcgaccata acgaacaaag aatggtgtac cgactttcag cccggcctgt ttcaggcccg
    33301 ccatcagcgg tggcaggatc tcttcgtagt tcacggtgat cgcatcagtt gacaggccat
    33361 cagaaataac gacctgcacg tccggattgg caacacactg cgctttcagc gcttcaacag
    33421 cttctgcaca caggcggcgg cccatatccg ggcgagtcag gtagagattt ttgtcgctaa
    33481 tttcagagcg tacttccagc aagccctgcg ctttcaccca ctcttccggc acttctttaa
    33541 gtacggtatc tttcgaacga gagtgatcgg ccaggaaacg cagcagcgcc tgggtacgcg
    33601 gacgcggacc ggcacgaccg gtacaaacgc gggccacggt gctgcgacgc agttctgtta
    33661 acacatccgc acgatgtggg ttttcaacac caatccacgc ttttgcttcc gcagaaccta
    33721 aatccagcgc gcagctttcc gaggtcaccg gtgccgcaca ggtggtagtg gcgcactttg
    33781 cttctgacgg cgcgggggcc gtttgtccca ttgacgccat cacgctgcgt acaatttctt
    33841 caatctgttt ttgatccatg atgtgttatc tccgcgtcat cagaagaaca gtgacggatc
    33901 gcccgcccgt ttggtcaggc gaccgtttgc cataatgccc atgctttcca gccagcgttc
    33961 aaactccggt gacgggcgca ggttgagtaa ctgacgcaca gtggcagtgt cgtggaaggc
    34021 ggtggtctgg tagttgagca tgatgtcatc gcccaacggc atccccatga tgtagttgca
    34081 gcctgcggtg gcgagcagga tcatcaggtt ttcgttgagg ttctggtcag cgtcagcgtg
    34141 gttggtgtaa cagcagtcac agcccataga gatgccgctc agcttgccca taaagtgatc
    34201 ttctaagccc gcacggataa tctggcggtc gttgtagaga tactccggcc caataaaacc
    34261 gaccacggtg ttgacgataa acggatcgta atgacgcgcc agcccatagt tacgtgcttc
    34321 catcgtcacc tggtcagcgc cgaagttagc gccagcggat agcgcagagc cttgtccggt
    34381 ttcgaagtag aggcagtttt ccccggcgat acggttgaac tctgcgccca ctgcgcgcgc
    34441 ttcgtcgagc atcgccagtt ccacgccaaa ctctttcagc cctttttcac tgccacaaat
    34501 actctggaaa atcagcccgc ctggtgcgcc acgacgaatc gcttcgatct gggtggtgac
    34561 gtgcgccagt acgcagccct gagttgggat gttgaatttg tcgatcacgc catagatggt
    34621 gtccagcacg cggcttaagt tttccacgtc gtcagttacc gggttgacgc cgatcaccgc
    34681 atcgcccacc ccgaaggaaa gcccttcgta gatttgcgcg gcgatacttt gcacgtcgtc
    34741 acgggtgtca tttggctgca aacgggcgct aaaggtgccc ggaataccga tggtggtatt
    34801 ggcctttttg attaccggca ttttcttcgc gccgtagatc aggtccgcgt tggagcaaat
    34861 cttcgctacc gccgcgacca cttccgaggt cagccctttg cgggtaaagg caatgtcgtc
    34921 cacgctggtt tcatcgctca gcacatactc acgcaattcg ctgatgctcc agtttttaat
    34981 ctggttgtag gccgtttcgt taacatcgtc ctgaatcagc cgcgtcacgc agtcatcttc
    35041 ataggcaatc accggattat tgcggatgtc cgctacggtc atttccgaca acacctgctt
    35101 tgccgccacg cgctcctgtg agcttgccgc tgcgacgccc gccagcacat cccccgaacg
    35161 cagttcgttg gctttagcca gcacctcttt tacatcctta aactgatata cattgccgaa
    35221 caatgtggtc tttagtttca taagtcgttc cctcaggaag gaaatgcgag tgatttcacc
    35281 gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg
    35341 cggacaatca cttcgtcaat gactgccaac gggagttgtt gtagctgtgg gcgcaacagc
    35401 atgcccagag ctttaccaaa gtcctgcccg gccaccacca gcaggggatg cggattcgga
    35461 aaacgcgcga cgaaatcgac cagcgcgttg atgaccgtca gtaccgcggc gtaacgcaca
    35521 ggcagcgagg cgggaagcgc cagcacgtac gcgtcagttt tgggatcaag atccagctga
    35581 atcagcgcct gttgccaggc acccaccaga tccgtttcat caatcgggat cgccaccggc
    35641 aaattgcgca gcggcagttg tacgccctcc agccagattg tgctgccaga gagcgaaagg
    35701 gtatgtgcac ccgcgccaat caccgtggcg cgcacggttt gcgccggaaa ctgcacgttc
    35761 atctcacgca ggcgcggatg gtcatgcagc gccgttgcca gcagcgggcc aatatcggca
    35821 aaacagaacg ggtcggcggg ctggtggcga taacattcgc ccacgccgcc agaaagcgta
    35881 atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt gcattaatgc ctgcgcgagc
    35941 ggcgaaagcg ttccgtcaat cacttcgaca atcagctctg ccatccgccg ggtaacctgc
    36001 accagctgag cgccggtcag cgaacgggcg tcggtgcctg cgccgaagca ctcatccaca
    36061 atcatctgcc ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg
    36121 cgaccaccga cgttgaggca ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag
    36181 ttcgcggtgc caccgccgat gtcgatattc agtacccgac acagccgttg ttcagaaagg
    36241 gtttgtgccc cggctccgtg accggcgatc acggattcga ggtgcggccc ggcgctggca
    36301 acgacaaaat cgcccagcga ctgagagagc gccatcaccg ccgggcgagc attgcgggtt
    36361 ttcgcgcttt caccggtgat gatgatggca ccagaatcaa cgctttccgg cgcaatgccc
    36421 gcagcctgat attgctctaa aatcagcgat ttcagttccg cttcttttaa accaccctgt
    36481 ttatcgacag gggtaaagaa caccgggctt tgccagctaa tttcgcgttt gatgaattcg
    36541 tagcgcggca cctgcgacac cgccgcacgg ttaaccagct ccaggtggga gaaaatcacc
    36601 tgggtggtgg tggtgccgat atcgataccg acgctcagta gctggcgagt gttcacgatt
    36661 gtgcctccgc ttcggtttta gccgcggtcg cgtcgtcttt tggcaccagc atcatcgcca
    36721 cgccaatcgc cgttacgccg ccgatcaact tgccgacaat catcgggaag atcatggcat
    36781 tcatgttggc agcggcgaag cctaagtggt cgcccagggc gaaagcagcg gaaacggcga
    36841 aggcgcagtt gatgactttg ccgcgggtat ccatctgctt catcatgccg aacatcggga
    36901 tgttgttggc aagcgttgcc accatgccgg ctgccgcgat gttgttcata ttcagcactt
    36961 taccgacgct catcagcggt ttttcaaacc agcgggtcag cagcagcacc atcggatacg
    37021 cccctaacag aacgcaggag atagaaccga taacttcaat ggcgcgcatc acctcaccgg
    37081 gtttatcgcc aggggccata aagataggat ccagaccggg gatcagttcc cagccaagca
    37141 ggaatttcac taccgcagcg gcaagaccga gggtgatcaa tgcaacgagg aatttggcga
    37201 agatctggaa gccgttgatc attttttccg ggatgaattt cagccccagc gccaccagaa
    37261 tcgcaacaat gatcaccggg atcatgttca tcaggatcag ggcgaaagtg aattccaccg
    37321 gctggccgtt gatctgaaca ccggagtaca tagctaccag accaccagcg atacaaccaa
    37381 tcggaatggt cacaatgccc gccagcacgc cgagcgccag ataacgacgg tcagaaggtt
    37441 cgataatgcc gagcgccacc ggaatggaaa acacaatcgt tggccccatc atcgacccga
    37501 gaattaaccc agagtatagc cacgctgcta cgtcgccgcc cgccagctct ttggcgagga
    37561 agaagccgcc catatcgcac gccagcagcg ttccggcgaa cattgacggg tttgcgccga
    37621 gcatttcgta aaccgggata attaccggcc cgagtacgtg agccagtacc ggtgccagcg
    37681 cggtcatacc gaccatcgcc aggcccagtg cgcccattgc cataaagcct tcttcgaact
    37741 gaccgcctga tccttcgata cttttaccga acttaccgag gaaacgagca gaaccgccga
    37801 actgcgacag gatcctgtct acggcagcta tcagcataaa gaacatcatg atgtacatga
    37861 tgatttcgtt aattcccatc gcctttactc cctgttagtt gttatttatt agcggatgct
    37921 gcgtgaagcc ttatccgccc tacatgtgca atcccgtagg ccggataaga cgcggcaagc
    37981 gtcgcatccg gcaattgcac cgcgccactg gcggatgctg cgtgaagcct tatccgccct
    38041 acatgtgcaa tcccccaggt cggataagac gcggcaagcg tcgcatccgg catttattgc
    38101 gccgctgcgt acagtccgac aatctgttcc aggctggcgg tacgcgggtt gctgcgcaga
    38161 caaatatctt cctgcgcggc ctgcgcccat gcaccgtaat gcgcagatgt cgcgccaaca
    38221 tcgcccagtc gtttaccaat cccaacttcc gcaatcagct cacttaccgc gttaatagcg
    38281 tcacgatcgt cggatttttt agttcgcagt gcccgaccaa tctgactaaa gcgttcacga
    38341 caaaccatcc ggttaaactc catcaccgtt ggcagcaaca tagcgttcgc gagaccgtgc
    38401 ggaatatgca gctccgcgcc cggctgatgc gccatcgcgt ggcacaaccc aagacccgca
    38461 ctggaaaacg ccattcccgc catacatgaa gccagcaaca tgctctcgcg cgcggcaagg
    38521 tcgtgaccgt agcccaccgc tttcggcagc gatttgccaa tcatcgcaat ggcaccaatc
    38581 gccagactgt cggtaaacgg tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt
    38641 aacgcatcaa tgccggtcat cgccgtgaca tgcgacggca caccctcggt caatgcggcg
    38701 tcgaggatcg ccacatccgg catcaacgag gcatgggcta acacctgctt gcgcccgctc
    38761 accgcgtcga taatcaccgt tacgttggtg gtttcagagc cggttccggc agtagtcgga
    38821 atggcaatca gcggcaagcg cggttgcaga acgctggttt ctgacatttc tgccagcgtg
    38881 ctatccgggt tcgtcaccag caacgccacg gcttttgcgg catccagcac cgagccgccg
    38941 ccaaatgcga tcaccccgtc acaacctgac tcacgcaact gcgccacggc tgcacacacg
    39001 tcggtaatgc acggttcgcc caccggacat ggccagagcg tcatggcgat acctttaacg
    39061 gccaggctac gcgtcagccc ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc
    39121 acgaacagat gtttcagccc acgcgtttgc gcttgctgcc cgcaactgct caccgcgccc
    39181 ggaccgcaaa gcgtcaccgg tggaacacta aatgttttta cccgttgcag attcagggta
    39241 tcgaacgcct gaaagagcgc ggtctgcaat tcattttgca tatagcccct ccgctttctc
    39301 tcgcccacta ctggcgatcg ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac
    39361 ctgtaacgcc gggaattgtt tgcgaaacag ttccgccacc cccggttgca tacaagagcc
    39421 gcccgccagc cataaatcag taatgccctg cccttcaata tgccgggcga caatgtccgc
    39481 cattttttcg tacaccggtt tcactgcggg ccaaatctcg tcaccgtgac cgcgcttgta
    39541 ctgctctgcc tcttccagcg aaatacggcg atttccggcg agagtcagag agatgtgatg
    39601 tccgccggtt gcttcatccg ccgagtacgt caccttgccc tttttcacga tggcaatgcc
    39661 ggtagtgccg ccgccgatat ccactacacc ggcgttgtcc agttgcagca gatccgcgac
    39721 tgcggttggc tcatccagca catggctcac ttccagtcca gcagactcca gcacgttgat
    39781 ggaaatacgc gggtccgtac ctggaggaaa tgaagtcgcc gcatggctaa aacggcgacc
    39841 gaattgctgc tcaagagtgt cgagatggcg acgaacaatg gtgacagcgc cgaagaaatc
    39901 ccagacgatg ccgtcgcgga ccacatcggc ccagtcgagg cataccgcca ccggctgacc
    39961 gtcgcggtcg acaaccatcg acaccacatc gcaggtaccc agatccaccc ccagccacag
    40021 cggggattcc gtcgcggcgg gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg
    40081 ggtgagccat tgttcgtcgt gcgccatcgg ttactcctta aacaatgcga aacgcatcga
    40141 ctaatacaca gcgacgcaga cggacaaacg tccgcgcgct ggttacccct tcaccggttg
    40201 gcgtggtgat tgtcatggtg gtccagcctt ccccgcccag ccccagcccg gcaatgcacg
    40261 gtccgttctt aacgaaaatg ctggtatcaa tagcgttcgc catctggttc atgttttcga
    40321 tgttgcgcga gtgcattgcc gccgtgtggt ggcaaccgcc ctccagtttc accgctagcg
    40381 caatggcatc cgccacgttg gcgacgcgca cgacgggcaa caccggcatc atcagttcag
    40441 tcacggcaaa cggatgttct gcggtggttt ccacaaacag caggcgcgtt tcttgcggaa
    40501 ctttaaggcc aattgccgcc gcgattttgc ctgcgtcgcg accaacccag tcacggctga
    40561 cggtgccttt tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct
    40621 gttctgcggt cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta
    40681 cgctatcaac aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagacg
    40741 cgcctttaac gatggactga gcagcacggg cgaggtcagc ggtttcatcc accactaccg
    40801 gcgggttgcc agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcta
    40861 ctaccgcttc gccgccggtc accaccagca ggccgatacc cggaaacttg aacaagcgtt
    40921 gcgcggtttc gatatccgga tttgccacag taaccagtaa gttttccggc ccgcctgcgg
    40981 caacaatcgc ctggttgagc agcgtgatcg cccgctggga gacttttttc gccgccggat
    41041 gcggggcaaa aatgacgctg ttgcccgcgg caatcaggct gatggcgttg ttaattacgg
    41101 ttgccgccgg gttagtggaa ggcgtcaccg aagccaccac gccccagggt gcgttttcaa
    41161 ttagggtcag gccgttgtcg cccgtcagca cttgcggaga gaggcactca acgcctggtg
    41221 tgccgcgcgc ctgagcgacg ttttttgcaa atttatcttc aacgcgcccc atgccggttt
    41281 cactgacggc aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag
    41341 caatggctaa ctggcgcatt gccacgctct ttaacccttg ctgggcaatt ttggctgccg
    41401 caacagcgtc atccagggac gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt
    41461 cactgctttg cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca
    41521 tgatgttctg ccttatttgt ggaaaattac ctgaccgcca gacaccacct catcgacgat
    41581 gccaatcacg cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact
    41641 gccactcacc agcaacaccc actccccggt tcccgcgcca atattgtcga tggcgacggc
    41701 acattgcccg tcaggattac cttgtggatc aatcatttcc accatcagca atttgtcatg
    41761 cgccagtccg tgatggcgta cggtacaaac aatttgtcca gtgacgactg ccagtttcat
    41821 acccgcctcc gtggcgtatt tcaggtaaaa gctcccccta ccctccgcgg aaggtaaaat
    41881 gaaaaatgag agagcgtgac gcccgaatcg acgtcacaca gggtgattac aggttgctgc
    41941 tatcgccttt caggccgatc gggaacactt cttccaggtc gccgtgcgga cgcgggataa
    42001 cgtgtacaga taccagctcg ccgatacgct gtgccgctgc ggcaccggca tcggtcgccg
    42061 ctttacaggc tgcaacatca ccacgaacca tggcagtaca caggccgccg ccaatctgct
    42121 taacaccaac cagcttgacg cgcgcagctt tcaccatcgc gtcagaagcc tcaatcagtg
    42181 caaccaggcc ccgggtttcg atcattccta atgcttccat tgtgctttcc tctttatcag
    42241 ggtccagaac gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc ggttcatttc
    42301 tgtctgacgc ggcacagctg ccaccagcgc cagctcgata atttcctgca cgctacaacc
    42361 acgagagaga tcgtgcatcg gcgcggcaag tccttgtatc aatggcccga cggcacgata
    42421 tccgccgagt cgttgtgcga ttttgtagcc aatatttccg gcttccagcg acggaaaaac
    42481 catcacattg gccttgccct gtagcgggct ggcaggcgct ttttgcgccg ccacttccgg
    42541 cacgaaggcg gcgtcaaact gcaactcgcc atcgactacc agctttggtg cgcgctcacg
    42601 gacgatttct gtcgcctgct ggacattggc aacgcagggg tgacgggcgc taccgttact
    42661 ggaaaacgac agcatcgcca cgcgcggctc ttctccggtg atggcgcgcc aggtttcggc
    42721 gctggcaagc gcgatatccg ccagctgcgc cgccgtcggc tgtggtacca cgctgcaatc
    42781 ggcaaagccc aacgcggggc cactgtactg tggcagcatc aggaaaatgg aagagagcgt
    42841 tttacagcca ggttgcaagc caataatgcg taatccggca cgcagcacat tcgccgtgga
    42901 agagaggttg cccgcgatac agacatccgc tttaccggcg ctgaccattg cggcggcgaa
    42961 catcagcggt tcggtgagtt tttccagcgc atccggcggc gttttttcgc ccgcgcgggc
    43021 cagccagcga tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat caatcacctg
    43081 tagcccgtcc atcgctacgc catgactgag cgcaaactga cgaagttcaa acggattggc
    43141 gaccataatg ggcgttgcca gaccttgttg atgtaaatat tgcgccgctt tcagcacacg
    43201 ttgatctaat gcatccggaa ataccactct ggcgggcgct cgcaacgcca gttcacgaca
    43261 acgttcaata atcattgcgt ttctcccagt cgttgttgga tctggttcac cgttagcggg
    43321 ctcttcgtta cgctcaggat catcatcacg taaaccgtgc tggaaaggcg gttcagcgcc
    43381 tgcaagatgt ccgggcgtaa tacttcaaaa ctgcgggtga taaacacctg cgccgctacg
    43441 gtttcggttt cacgcacttt ggtgcgtagc agattcagca acgccgcatc gcgcccgtgg
    43501 ctggcctccg gcaccagatg atcgtgacca agataacgca gcggctggtg agaaagccga
    43561 tgcagatctt cgtcagacag cccgacaatc gcctggcagc ccagcggttc ccccagcgca
    43621 tcggcacgca taatgttgcc aagacgcgaa cggatatccg ccagccacgg ctgccacggt
    43681 tctgccagtt caatttgcag ccacactgcc agcgcaatag tgctgtcgag aacagcgcga
    43741 aaacccagac gcggatcgct tttggcgacc attttttccg ccgacagatg agtcagcgta
    43801 tccggctttt ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg atgttcatcg
    43861 ctactggtca gcccatgaac gggctgcggc tgctgctgtt catcgtcaac aaacaggcgg
    43921 ccctgctcgt caataaactt gatgcgcaga tggcggcttt ccagcaactc ccgggcagag
    43981 ggcgtcaggc gactgtccgc agggagatgg atctctgcgc cttcgctgag cgtatggttc
    44041 gctcttagcc atgcttcggt gatgaaatct ttcataggga ttgccagtta gcaggccagg
    44101 cgacatacag gaatttcacg ctggatgtcg taccaaattc gatactggag cctttcggga
    44161 taaacatcac gtcgcctgct ttggcgatca tggtttcgcc ttcgtggcga acatgcagct
    44221 ccccttccag aaccatatcg atttcgtcgt agttcagggt ccacgggaag aatgcgttgt
    44281 cccactgcat aaaaccagcg gccatgctgc tgccgtcgtc tccggtcaca aggtcagtta
    44341 agcccacgca atgcggctct gcgccgtcga aacgaccaaa cttgacgctg ctgccatcaa
    44401 tcactttgat gccgcctttg ccggtcaccg atttgaagct cggctgcatt gcaccctgct
    44461 ccagcgactg tttttccttc atcacttttt ccatcaactg cgcgaccagg ctttcggtaa
    44521 actggccttc cggcagttgg gcgataatgg tttcgcggat gcgctggctt tcggttttat
    44581 cggctggcac gctggcgggc acggacgccg ttaccgggat caattcatca cattcggtga
    44641 tggtaaaacc cagtagatcc gccacttcac gggcttccgg ggtaataatg ctggcgcgca
    44701 gaaccacgga cattgcctgt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga
    44761 tgagtttttt cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc
    44821 tgcacgcttt gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa gccagtctca
    44881 agcaataact ttcgcgttgc ggcgacgtca gcatctggca tgtccgtttt actgatgacg
    44941 gcgatttgcc gtttgctgac gccaatatcc agtaacccgg caggcaaccg actttccggg
    45001 tcattcgcgc cgtggacata gatcagcatg tccacatcct gtagcgtggt aattaaggcg
    45061 tgataccagc ggggatggct aaaatattca cccggcgtat caatatcgcc cttatcatta
    45121 aattccacgg cctgtgtttt tctggcgagg gtataattcc cctgcaacgc attaaataac
    45181 gttgtttttc ctgccccgac ggtacccaca aacgcaattc gtttcatgaa tgcctcgtat
    45241 taacttttgg tcatttcaca aagcgtataa tttaataagc gacctaaacc gctgacggtt
    45301 tgtgataacg cttcttctac tgcgcccacc gagccataaa tcaccaatgc accactgaag
    45361 cggtcgagaa aaccgatatg cacatcagcg gctttcaatg ctaaatcacc ggcgatcatc
    45421 gccgtttcgc caggagttag cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt
    45481 ttcttcgcca gttcctcgcc gggatgcgcg atgagatgcg ccagcgtgac ctgtttgccg
    45541 ggcacaaatt cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaactttc
    45601 gtgtatcgtg acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag
    45661 gtgaggtaga tcactaagaa tggagagaga ttcgctgtgg tgcataaact ttcatataca
    45721 acgcatgaat ataaataaaa taatatcgag ataatatata gactgctttt ttttattatt
    45781 cgcaaactca gtaataagct ttactaatat attgcagcca aaccgcttac cctgaatatt
    45841 cagggtaagc gtgagagtta aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg
    45901 ccagcgccac catgttgacg atacgacgca ccgatgcgat cggcgttaac acgtgaaccg
    45961 gtttcgccac gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca
    46021 gtaagttgta actaatgcgg gcagcttcca tgtttggcat taccagaata ttggcagaac
    46081 ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc
    46141 cgtgcatttc accatcaatc atcagttctg gcgcacgttc cctgaccagt tccagcgcct
    46201 gacgcatttt gctcgacgac gggcaatcag aagaaccaaa gttggagtgc gacaacagag
    46261 caacgcgcgg ctcaatacca aaacgacgga cagtttctgc cgccatcaag gtgatctccg
    46321 ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc ggcaataaag gtgttaccac
    46381 tcggcagcag cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga
    46441 catttttcac cacgctaaaa tgttcatgat aatcacccac cgtaccgcaa atcattgcat
    46501 cggcttcgcc acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcaacgccc
    46561 gctgcgcctg ttcctgagtg acgccgcgac gcttcatgat ctggaagtat tcggtccagt
    46621 actctttaaa gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgctttga
    46681 tctgcaagcc cagtttctga atgcgcattt cgatcacgtt cggacgaccg ataaggatcg
    46741 gtttcgccag tcccagcgtt accagttcct gagtggcatg cagaacgcgc gcctcttccc
    46801 cttccggcag aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa
    46861 acaggttggt tttgtaaacg aactcagtca gcttgtcgat gtagacgtcg aaatcagcaa
    46921 tcggacgcgt cgccacgccc gactccatcg ccgctttcgc gaccgcagga gcgatcttaa
    46981 cgatcaagcg cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct
    47041 gatcgccata cgctgaagcc accacttcgc tctgttccgc atgggccagt tctgcaatcg
    47101 cgcgtaccgc cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc
    47161 cacggaagat gaacgggaag caaaggacgt tgttcacctg gttcgggtag tcggaacggc
    47221 cggtacagat gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt
    47281 ccgggttcgc cagcgccagg atcattggcg cacgagccat tttcttcacc atttcctggg
    47341 tcagcacttt cgggccggaa cagccgagga aaatatccgc gccttcaatc acatcatcga
    47401 gggtacgttt gccgtcatcc actaccgcat atgcggcttt ggtttccgcc atgtttggct
    47461 cacggccctg atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac
    47521 ccagcgcgac cagcaggttc atacaggcga ttgctgcagc acccgcgccg gaaaccacca
    47581 tacgcacgtc agagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc
    47641 tgataattgc cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca
    47701 gtttctgttc aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccgccga
    47761 aggtcggttc gagcgcagcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt
    47821 caatgtcaaa tacatcaatt ccggcgaatt tcttaaacag aacgcccttg ccttccatca
    47881 ccggtttgcc cgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga
    47941 tcaccgccac caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga
    48001 tttcaagaca aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg
    48061 ccagaggctt ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa
    48121 gtgcactttg ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat
    48181 aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca cattattacc ttgccggagt
    48241 ctacagactt tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct
    48301 ataacactga tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg
    48361 atgcgtttag cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt
    48421 tcaaggagta tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc
    48481 agacagcggc gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc
    48541 ttcgctgtta ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat
    48601 cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa
    48661 actggcggtc aatttcggtg ctgaaatcct caaaatcgta cccggtcgcg tgtcaacaga
    48721 agttgatgca cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt
    48781 ggacttgtat cagcaacaag gcgttgagaa atcacgcatt ctgatcaagc tggcttcgac
    48841 ctgggaagga attcgcgcgg cagaagagct ggaaaaagaa ggtattaact gcaacctgac
    48901 gctgctgttt tcttttgcgc aggcacgggc ctgtgcagaa gcaggcgttt ttctgatttc
    48961 gccgtttgtc gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt
    49021 ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc tacgactact ataagcaaca
    49081 ccactatgaa accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt
    49141 aaccggctgc gatcgactga ctatcgcacc gaatttgctg aaggagctgc aggaaaaagt
    49201 ttcgccagtg gtacgtaaat taatcccacc ttctcagacg ttcccacgcc cagctcccat
    49261 gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc
    49321 tgaaggcatt cgtctgttcg ccgttgatca acgcaaactg gaagatcttc ttgccgccaa
    49381 actataaacc agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg
    49441 cactcagtat ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca
    49501 tggctgatat tgccgaagtg ctgtggaacg attttcttaa acataaccct accgacccaa
    49561 cctggtatga tcgcgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca
    49621 gtttgctgca tctgaccggt tacgacctgc cgctggaaga gctgaagaac tttcgtcaac
    49681 tgcattcgaa aacccctggc cacccggaaa tcggctatac gcccggagtt gaaaccacca
    49741 ccggtcctct tggacaaggt ttggcgaacg ccgtcgggct ggcgatagcg gaacgtacgc
    49801 tggctgcaca gtttaaccag ccagaccatg agatcgtcga tcacttcacc tatgtgttta
    49861 tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt ctgttcgctg gcgggcacgc
    49921 tgggactggg caagctgatt ggtttttacg atcacaacgg tatttcgatt gatggagaaa
    49981 ccgaaggctg gtttaccgac gatacggcaa aacgttttga agcctatcac tggcatgtga
    50041 tccatgaaat cgacggtcac gatccgcagg cggtgaagaa agcgatcctt gaagcgcaaa
    50101 gcgtgaaaga taagccgtcg ctgattatct gtcgtacggt gattggcttt ggatcgccga
    50161 ataaagcagg taaggaagag gcgcacggcg caccgctggg ggaggaagaa gtggcgctgg
    50221 cacggcaaaa actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg
    50281 cctgggatgc ccgcgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg
    50341 cctataaaaa agctcatccg caactggcag aagagtttac ccgtcggatg agcggtggtt
    50401 taccgaagga ctgggagaaa acgactcaga aatatatcaa tgagttacag gcaaatccgg
    50461 cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc
    50521 ctgagctgct cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt
    50581 ctgtttcgct gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg
    50641 gcatgaccgc tatcgccaac ggcattgcgc accacggcgg ctttgtgccg tataccgcaa
    50701 cgttcctgat atttgttgaa tacgcccgca acgccgcgcg gatggcggca ctgatgaaag
    50761 cgcggcagat tatggtttat acccacgact caattggtct gggcgaagat ggtccgacgc
    50821 accaggctgt tgagcaactg gccagcctgc gcttaacgcc aaatttcagc acctggcgac
    50881 cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc ggttgagcgc cacaacggac
    50941 cgacggcact gattctctcc aggcagaatc tggcccaggt ggaacgtacg ccggatcagg
    51001 ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta
    51061 ttttgattgc caccggttca gagatggaaa tcaccctgca agcggcagag aaattagcgg
    51121 gagaaggtcg caatgttcgc gtagtttccc tgccctcgac cgatattttc gacgcccagg
    51181 atgaggaata tcggaagtcg gtgttgcctt ctaacgttgc ggctcgcgtg gcggtggaag
    51241 caggtattgc cgattactgg tacaagtatg ttggtctgaa aggggcaatt gtcgggatga
    51301 cgggttacgg ggaatctgct ccggcggata agttgttccc gttctttggc tttaccgccg
    51361 agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa aggtgcctga tggtgatcgc
    51421 cggatgctga tcgccggatg ctgattgccg gatgcgacgc tgacgcgtct tatccggcct
    51481 acagcgtctt atccggccta catgtcccgc cattctgttt aacgggtgat ccacaacgtg
    51541 ggccaggcgt ctggcccatg ccagttatcg caggtgggtt ctgcggcgta acgcaccagg
    51601 cgaaaacgct gaccgtcaaa gcgccagcgc gcctgaatgc cacaatcgct taatccgcgc
    51661 cctttcgcta aggtcaccag ttcacgcgat ttctcatcaa atgttgcgtt catcagttcc
    51721 agttcattcg tctcctgccc gttgttgaac ggcaaacgca accgaaccgg acgcgaggcc
    51781 agtggctttt tacgcgacac aatccatgcc aaatcaatgg tgttataggc ccctgcctca
    51841 cagctaatca tcatcagcgc tttatcatca gtcagcgcag tgacattcac ctcacgacgt
    51901 aacggatcaa gcgagcagcg cagaccattc atccgccagt ttccataatc cagcaaatcg
    51961 ttgcgttctt cgagtgagag tggcgtcggc gttgggttaa ccaccgcgac ctctttcagc
    52021 gcaggcgcgg gcggtacgct gagcggcggt tcgtcccctt tctttatcca cgcggtttca
    52081 ctgccaacgc gcttttgctg agcatcaata aacaacaacg ccgcttttaa gccactcaga
    52141 gaaatggtct gattgccgtc gcgtaaggtg atcgccttcc cttcctgaat catctgcaaa
    52201 aacgcggtga tggttgccgt atcatcggtt actagtaacc atggtgaaat ccgccacttg
    52261 tcaccactta acgccaacgg ctcaccatct aacagcagcc gtggcgcaat ttccccttca
    52321 gacgcatccg gcgacttcaa cccgccgcgc tcaatacgta aaacggcatc ggtatgcgcc
    52381 ccggcgctgc ggctcagggt catcaccagt ccattatgat cgcccgtatt acgcgccacg
    52441 cagaagtttt ggttattaca ggtgacctgc cagtcggaaa acgcccgttg cgctggtgcc
    52501 gcccacacca aagacgtcgg caacaaagcg aaaaaaaaga gaaggaaaat gcgatagcgc
    52561 atggacggta cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag
    52621 gtatcgcggc tcaatcggat ttgtcggatt gagccaacaa taaacaacga aatattattg
    52681 ttatttttca gtccattaaa tgtgacgttt gcaaatagtt aagcaataac accgtcttac
    52741 cgtcacgtat ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca
    52801 cttcaatatc ttcatcttcg acaccgccac cggcgttagc gcgctgattg tcactgtatt
    52861 cggcgataaa aaagtggatt agctcagtca caccgcctgg cgacatatac agttcaaata
    52921 atttgcgcac ttcacccact tcatagcccg tctcttcaat cgcttctttg cgaatgcaca
    52981 cttccggttc gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat
    53041 tgccattaac ccaagtagcg acacggaact gacgaatcag aaccacggtc tttttcttcg
    53101 cgttgtacag gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga
    53161 taacttcgcc atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat
    53221 cggagagaat tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg
    53281 aaatgaacaa ttatcttacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat
    53341 ttgactgatt ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc
    53401 attgcggtaa ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga
    53461 ttggtggtct ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct
    53521 accgttacac cgtgcgactc cagccacggc atcccgtgcg gattgaagcc aaacgccata
    53581 atcaccgcgt cggctggcat gacaaattca ctgccttcca ccggcactgg acgcctacgc
    53641 ccctgggcat ccggctcgcc aagacgcgtg cgcaggaaac gaatcccgca aacgtgacct
    53701 tgttcattca gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg
    53761 cgggcgttct tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg
    53821 caggtgacgt tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg
    53881 ccgccgccca gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc
    53941 tcttcgagcc ccatcacctg tttagtgttg gcgatgagga acggcagcgc gtcataaacg
    54001 cctggcgcat cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg
    54061 aagaccgcgt cgtattgttc caaaagcgaa tccaaagaga catctttacc cacttcacaa
    54121 ttgagttcga agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat
    54181 ttatccagtt tgaaagaagg aatgccgaaa gtaagcaagc caccgatttc tggatggcga
    54241 tcgtacaccg tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc
    54301 ggacctgcac cgataatcgc cacccgcttg tccactttgg tgacatggct taagtcaggg
    54361 cgccaacctt tcgccaacgc ctgatctgaa atgtagcgtt caatgttgcc gatagttacc
    54421 gcaccgtgct catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg
    54481 cgtccggtaa tttccggcag ggtgttggtc aggtgagaaa gctcgacggc ggcgtcgatg
    54541 tttccggctt tcaccagttc aatccactgc ggtatatggt tatgcagcgg gcaggtccat
    54601 tcacaaacgc tgtgctcgcc gcatttaagg cagcgcgagg cttcccgttg tgcctgatcg
    54661 gcacggaatg gcagataaat ttcatcaaaa ccggttttgc gcgcttcaat cgccagttta
    54721 tccggctcgc cacgcgcggg cgttgcctgc atttgttcga ctttactcat taccggcatt
    54781 tcttgtgcag cggtactggc atgccacggt tgatgttcct gacgcgcggt gcgcaagcgg
    54841 cgggatttcg ccatgccgga gagtgcggcg tcagtgacca gttgcagcgc gtccgccggg
    54901 caattctcga cacaggcagg accgttttcg cgccctgcac aaaggtcgca tttatgcgcc
    54961 gtggctttaa ctttgcccgc tgcgacgggt gtcaggacga tttgcatcgt accaaacgga
    55021 caggccacca cgcaggattt acagccaata cacttttgct gattgacctg aatgctgtca
    55081 tcaacgtggc tgattgcgcc attagggcag ctacgggcgc agggcgcatc ttcacaatgg
    55141 tgacaggtca ctgcactacg ttgctgctga tgtttgataa ccgtaattcg gggatgaaaa
    55201 tggtgttggc tcaggacatg ttgctcatca ttgtgagcca tgacacaggc gatttcacaa
    55261 gcatgacaac ccagacattg ctgactgttg gccataataa aacgattcat aacgaccttc
    55321 ttttttggtt gtaaaaacct tattctttat atgagtgttg ttattacccg acttacaggg
    55381 gaatcggcaa tgttcatgtg cccagaataa gtaactattt cgctataaac tgtggcagat
    55441 caaataatcc catcactgac taaattgcgt ttcagcgaac tggaacatta atgatttttt
    55501 gtggagaaga cgcgtgtgat tgttaaacga cccgtctcgg ccagtctggc ccgggccttt
    55561 ttttacattg tgctgctgtc gattctttcc acgggtatcg ctctgctaac tctggcgagc
    55621 agtttgcgcg acgctgaggc tatcaatatt gccggatcgc tgcgtatgca gagttaccgc
    55681 ctgggctacg acttgcaaag tggcagtcca caactcaatg cacatcgcca gttatttcag
    55741 caggcactgc attcaccggt attaaccaac ctcaacgtct ggtatgtgcc agaggcagta
    55801 aaaactcgct atgcgcatct gaatgctaac tggctggaga tgaataatcg cctcagcaag
    55861 ggcgacttgc cgtggtatca ggccaatatt aataactatg ttaatcagat agacctgttc
    55921 gtgctggctt tacagcacta cgctgaacgc aaaatgctgc tggtggtggc gatttccctg
    55981 gctggcggca tcggtatttt cacgctggtc ttttttactt tgcgccgcat acgccatcag
    56041 gtggttgccc cgctgaatca gctggttacc gccagtcagc gtattgaaca cggacagttc
    56101 gactcgccac cgctggatac cagcctgccg aatgagcttg gcctgcttgc aaaaaccttt
    56161 aaccagatgt cgagcgagct gcataaattg taccgttcgc tggaggcgtc agtagaagaa
    56221 aagactcgcg atctccacga ggccaagcgt cgtctggagg tgttgtatca atgttcgcag
    56281 gcgctgaaca ccagccagat tgatgtgcat tgtttccgcc atattttgca gattgttcgc
    56341 gacaatgaag cggctgaata tctggagtta aatgtcggtg aaaactggcg gattagcgaa
    56401 gggcaaccaa acccggaatt gccgatgcag attttaccgg tgacaatgca agagacggtt
    56461 tacggcgaac tgcactggca aaatagtcac gtttcatcat cagaaccgct gcttaacagc
    56521 gtttcgtcga tgctgggacg cggtttgtac tttaatcagg cgcagaagca ttttcagcag
    56581 ttattgttga tggaagaacg tgcaaccatc gcccgcgaat tgcacgactc gctggctcag
    56641 gtactttctt acttacgtat ccagttgacg ttacttaagc gttcgatacc ggaagataac
    56701 gccaccgcac aaagtatcat ggccgatttt tcccaggcgt tgaatgatgc ttatcggcag
    56761 ttacgcgagc tgttgactac tttccgcctg acgctgcagc aggcggatct cccctccgcg
    56821 ttgagggaaa tgctggatac gttacaaaat caaaccagcg ccaaactgac cctcgactgc
    56881 cgtctgccaa ccctggcgct ggatgcgcaa atgcaggtgc atttgttgca aattattcgc
    56941 gaagcggtgc tgaatgcgat gaagcacgcc aacgccagcg aaatcgccgt cagttgcgtc
    57001 accgcgccgg acggcaatca cacggtttat atccgtgata acgggattgg tatcggtgaa
    57061 ccgaaagaac ccgaaggtca ttatggtctg aatatcatgc gcgaacgcgc ggaacggcta
    57121 ggtgggacgc tgactttttc acaaccttcc ggcggcggca cgttagtgag tattagcttt
    57181 cgctctgcgg agggtgagga aagtcagttg atgtaatgcc tcctactgac caaagaatac
    57241 ttgcacttaa ggttcagtat aaaagggcat gataatttac attaactcct ttttttctcc
    57301 acgattggct cgtaccttgc cgctacagtg aagcaagtca agcctacaac gatacgcaga
    57361 aacacgaggt cctcttttaa tggcgaattt ctttattgat cgccccattt ttgcctgggt
    57421 gctggcaatc ctgttgtgtc tgacaggtac cctggcgatt ttttcattgc ccgttgaaca
    57481 ataccccgat ctcgcgccac cgaatgtgcg agtgaccgct aactatcccg gcgcatcggc
    57541 ccagacgctg gaaaacaccg tgacccaggt tatcgagcaa aatatgaccg gcctcgataa
    57601 tctcatgtat atgtcatctc agagcagtgg caccggtcag gcatctgtca ctttaagttt
    57661 taaagcaggc accgatccgg acgaagccgt gcagcaagta caaaaccagc tgcaatcagc
    57721 catgcgaaag ttaccgcagg cggtgcaaaa tcagggcgtg acggtgcgta aaactggcga
    57781 taccaatatt ctgaccattg ctttcgtctc taccgatggt tcgatggata aacaggatat
    57841 tgccgattat gttgccagta atattcagga cccgttaagc cgcgtgaatg gcgtcgggga
    57901 tatcgatgcc tatggttcgc aatattccat gcgtatctgg ctggacccgg cgaaactcaa
    57961 cagtttccag atgacggcta aagatgtcac tgatgccatt gagtcacaga acgcgcagat
    58021 tgcggttggg caacttgggg gtacaccttc cgtcgataag caggcgctca acgccaccat
    58081 taacgcccag tcactgctgc aaacaccaga acagttccgc gatatcacct tgcgggtgaa
    58141 tcaggacggc tcagaggtaa ggctgggcga tgtcgccacc gtcgaaatgg gggcggagaa
    58201 atacgattat cttagccgct tcaatggtaa gccagcctcc gggctggggg taaaactggc
    58261 ctccggcgct aacgaaatgg cgacagcgga gctggtgctc aatcgtctcg acgagctggc
    58321 gcagtatttc ccgcatggac tggaatacaa ggtggcgtat gaaaccacct cgtttgttaa
    58381 agcctccatt gaagacgtgg tgaaaacgct gctggaagct atcgctctgg ttttcctcgt
    58441 tatgtatctg ttcctgcaaa acttccgcgc cacgctgata cccactatcg ccgtgccggt
    58501 ggtgttgatg ggaaccttct ccgtacttta cgccttcggt tacagcgtca acaccttaac
    58561 catgttcgcg atggtactgg cgatcggtct gctggtggat gacgccatcg tggtggtgga
    58621 aaacgtcgaa cgtattatga gtgaggaagg actcactcct cgcgaagcca cacgtaaatc
    58681 gatggggcag atccaggggg cactggtcgg gattgcgatg gtgctttcgg cggtatttgt
    58741 accaatggcc ttcttcggcg gcaccaccgg tgccatctat cgccagttct ctattaccat
    58801 tgttgcggcg atggtgctgt cagtactggt agcgatgatc ctcactccgg ctctgtgtgc
    58861 cacgctactt aagccactga aaaaaggtga gcatcacggg caaaaaggct tttttgcctg
    58921 gtttaaccag atgtttaacc gcaacgccga acgctacgaa aaaggggtgg cgaaaattct
    58981 ccaccgcagc ctgcgctgga ttgtgattta tgtcctgctg cttggcggca tggtgttcct
    59041 gttcctgcgt ttgccgacgt cgttcttacc gctggaagac cgtggcatgt ttactacctc
    59101 ggtacaattg cccagcggtt caacccaaca acagaccctg aaagtcgttg agcaaatcga
    59161 gaaatattac ttcacccatg aaaaagacaa catcatgtcg gtgtttgcca ctgttggttc
    59221 tggccctggg ggtaacgggc aaaacgtggc gcgaatgttt atccgcctga aagactggag
    59281 cgaacgcgac agtaagaccg gcacctcatt tgccattatc gagcgtgcaa cgaaggcgtt
    59341 taacaagatt aaagaagctc gcgttatcgc cagcagcccg ccagcaatta gcggtcttgg
    59401 tagttctgcg ggttttgata tggagttgca ggaccacgct ggagcaggtc acgatgcgct
    59461 gatggcagca cgtaaccagt tgctggcgct ggcggcggaa aacccgaagc taacccgtgt
    59521 gcgccataac ggcctcgacg acagtccgca gttgcagatt gatatcgacc agcgtaaagc
    59581 tcaggcgctg ggcgttgcta tcgacgatat taacgacaca ctgcaaaccg cctggggttc
    59641 gagctatgtg aatgacttta tggatcgcgg tcgcgtgaag aaagtctatg tgcaggcagc
    59701 tgcgccgtat cgcatgctgc cagatgacat caatctctgg tatgtccgaa ataaagatgg
    59761 cggcatggtg cccttctctg ctttcgcgac ctcacgctgg gagacaggct cgccgcgtct
    59821 ggaacgctat aacggttatt ctgcggttga aattgttggg gaagccgcac cgggggtcag
    59881 taccggtacg gcgatggata ttatggaatc gttagtgaag cagctgccaa acggctttgg
    59941 tctggagtgg acggcgatgt cgtatcagga gcggctttcc ggcgcgcagg ctccggcgct
    60001 gtacgccatt tccttgctgg tggtattcct gtgtctggct gcattgtatg aaagctggtc
    60061 ggtaccgttc tcggtaatgc tggtcgtgcc gctgggggta atcggcgcgc tgctggcaac
    60121 ctggatgcgc gggctggaaa acgacgttta cttccaggtg ggcctgttaa cggtcattgg
    60181 tttatcggcg aaaaacgcca tcctgatcgt cgagtttgct aacgagatga accaaaaagg
    60241 ccacgacctg tttgaagcaa cgctccacgc ctgccgtcag cgtttacgcc cgattctgat
    60301 gacctcgctg gcatttatct tcggcgtatt gccaatggca accagcacgg gtgccggttc
    60361 cggtggtcag catgcggtgg gtactggcgt aatgggcggg atgatttcag ctactattct
    60421 ggctatttac ttcgtgccgc tgttctttgt gctggtgcgc cgccgcttcc cgctgaagcc
    60481 gcgcccggaa taagcaataa aaaaggcgac atgctaatgc gtcgcctttt tcaactttcc
    60541 gattaagaac ctgctcagcg ggttcttgct gtttgtactt tgtctcagga attacttacg
    60601 aagcataact tcgataaagt ctttccagtt ccccagttca cgttcaatca taacaacctc
    60661 tcttataatt atgggtattc tacggaaaca atataccgtg gtgaagctaa tttactcgat
    60721 tgctgcgatg actacctccg ggggacaaac cttatgtaaa tactatggtc ctgcggtgat
    60781 gatttgtatg tgatacacag caacatttcg agatattcat acggcatcta atactgatt
//
LOCUS       DAPGRN010000015        57417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPGRN010000015 DAPGRN010000000
VERSION     DAPGRN010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.015"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            34..600
                     /locus_tag="RHW33_001036"
     CDS             34..600
                     /locus_tag="RHW33_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1644569.1"
                     /translation="MFKGQKTLAALAISLLFASPVFAADEGSGEIHFKGEVIEAPCEI
                     HPDDLDMNIDLGEVTTSHINREHHSEKKPVNIRLINCDIAGWDDGKGGVVSKVGVTFD
                     STAKTTGATPLLSNISAGEATGVGVRLMNKDESFVVLGTEAPTIDLLASSTEQTLNFF
                     AWMEQMDNAVSVTAGAVTANATYVLDYQ"
     gene            650..3109
                     /locus_tag="RHW33_001037"
     CDS             650..3109
                     /locus_tag="RHW33_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1644570.1"
                     /translation="MDAVNIYRLSVLSCLAMVTPPALAAEFNLNVLDKSIRDSVDISL
                     LNQKGVVAPGDYFVSVTVNNNKISNGQQIRWQKSGDKIIPCINESLIELFGLKSDFRK
                     KLPAIKECVDFSVFPEIIFTFDQANQQLNITIPQAWLAWHSENWTPPSTWNNGIPGFL
                     MDYNLFASTYRPQSGSSSNNLNAYGTTGLNAGAWRLRSDYQLSQSDSGDNREQSGAIS
                     RTYLFRPLPQIGSRLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISHSGRVIYQKKVPPGPFIIDDLNQSVQGSLDVKVSEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGRPRSSMSHHTEDETFISHEVSWGMLSNTSLYGGMLLAGDD
                     YRSGALGIGQNMLWLGALSFDVTWADSHFDIQQDEQGYSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYIDHKYNDADAQDEKQTISLSFGQPITPLNLNLYANILHQSWWN
                     ADTSTTANITAGFNVDIGDWKDISVSTSFNTTHYEDKDRDNQIYFSISLPIGESGRLG
                     YDMQNNSNTTTHRMSWNDTLDERNSWGMSAGIQSDRPDNGAQVSGNYQHLSSAGEWDL
                     TGTYAANDYTSASASWSGSFTATQHGAAFHRRSSTNEPRLMVSTDGVGDIPIQGNIDY
                     TNRFGIAVVPFVSSYQPTTVAVNMNDLPDGVTVSENVVKETWTEGAIGFKSLASRAGK
                     DLNVIISDANGHFPPLGADVRQAEGGVSVGMVGENGHAWLSGVDENQQFTVHWGDQKT
                     CAIHLPEHLEDVTKRLILPCH"
     gene            3124..3856
                     /locus_tag="RHW33_001038"
                     /pseudo
     CDS             3124..3856
                     /locus_tag="RHW33_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            3853..4914
                     /locus_tag="RHW33_001039"
     CDS             3853..4914
                     /locus_tag="RHW33_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644571.1"
                     /translation="MRFAKGVLLAICLIFLPLKAALALNCYFGTANGAVEKSEAIMPF
                     AVPANSKPGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGIQDPYYQLGVTY
                     EGVDYDASLGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKLCSGDPTVMHKSRTFVA
                     RMRLYVKVRAMPPHDYQSKLSDYIVVQFDGAGSVNEDPTAKNLKYHITGLENIRVLDC
                     SVNFAISPETQVVDFGRFNVLDIRRHTMSQQFKITTTKSQNDQCTDGFKVSSSFYTDE
                     TLIDEDKSLLIGNGLKLRLLDENASPYTFNKYSEYADFTSDLLVYEKTYTAELSSTPG
                     TPIDVGPFDTVVLFKINYN"
     gene            5063..6109
                     /locus_tag="RHW33_001040"
     CDS             5063..6109
                     /locus_tag="RHW33_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV1644572.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQHIVWFPPVSINLLLTILLAVVAGTVGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRTA"
     gene            complement(6106..6897)
                     /gene="nei"
                     /locus_tag="RHW33_001041"
     CDS             complement(6106..6897)
                     /gene="nei"
                     /locus_tag="RHW33_001041"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV1644573.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEESQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6933..7667)
                     /gene="pxpA"
                     /locus_tag="RHW33_001042"
     CDS             complement(6933..7667)
                     /gene="pxpA"
                     /locus_tag="RHW33_001042"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV1644574.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGLLVPRNQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKGIVVAA"
     gene            complement(7657..8589)
                     /gene="pxpC"
                     /locus_tag="RHW33_001043"
     CDS             complement(7657..8589)
                     /gene="pxpC"
                     /locus_tag="RHW33_001043"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV1644575.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8583..9239)
                     /gene="pxpB"
                     /locus_tag="RHW33_001044"
     CDS             complement(8583..9239)
                     /gene="pxpB"
                     /locus_tag="RHW33_001044"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV1644576.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9262..10005)
                     /gene="ybgI"
                     /locus_tag="RHW33_001045"
     CDS             complement(9262..10005)
                     /gene="ybgI"
                     /locus_tag="RHW33_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV1644577.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10276..11757
                     /gene="dtpD"
                     /locus_tag="RHW33_001046"
     CDS             10276..11757
                     /gene="dtpD"
                     /locus_tag="RHW33_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV1644578.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGVVLMIWLYQALKFRNRALALES"
     gene            complement(11907..13325)
                     /gene="phrB"
                     /locus_tag="RHW33_001047"
     CDS             complement(11907..13325)
                     /gene="phrB"
                     /locus_tag="RHW33_001047"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV1644579.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVELVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKSVHEPWKWAEKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(13322..13831)
                     /locus_tag="RHW33_001048"
     CDS             complement(13322..13831)
                     /locus_tag="RHW33_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV1644580.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANRRDHTNVLMHVQGY
                     FRPHINSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13932..14138)
                     /locus_tag="RHW33_001049"
     CDS             complement(13932..14138)
                     /locus_tag="RHW33_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HDV1644581.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            14450..14539
                     /gene="kdpF"
                     /locus_tag="RHW33_001050"
     CDS             14450..14539
                     /gene="kdpF"
                     /locus_tag="RHW33_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV1644582.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            14539..16212
                     /gene="kdpA"
                     /locus_tag="RHW33_001051"
     CDS             14539..16212
                     /gene="kdpA"
                     /locus_tag="RHW33_001051"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV1644583.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALVIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            16235..18283
                     /gene="kdpB"
                     /locus_tag="RHW33_001052"
     CDS             16235..18283
                     /gene="kdpB"
                     /locus_tag="RHW33_001052"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV1644584.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAVADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAVTYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            18292..18864
                     /gene="kdpC"
                     /locus_tag="RHW33_001053"
     CDS             18292..18864
                     /gene="kdpC"
                     /locus_tag="RHW33_001053"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV1644585.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPNASTSVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            18857..21541
                     /gene="kdpD"
                     /locus_tag="RHW33_001054"
     CDS             18857..21541
                     /gene="kdpD"
                     /locus_tag="RHW33_001054"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV1644586.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGVQAE
                     IGIDAHVEGENLQLDVWDNGPGLPQGQEQSIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            21538..22215
                     /gene="kdpE"
                     /locus_tag="RHW33_001055"
     CDS             21538..22215
                     /gene="kdpE"
                     /locus_tag="RHW33_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV1644587.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(22328..22822)
                     /locus_tag="RHW33_001056"
     CDS             complement(22328..22822)
                     /locus_tag="RHW33_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644588.1"
                     /translation="MKTKRSLVVRVAFIAATLLLTACAQPEQSSLAGDWLPTHVDKKQ
                     VLEGSAVLKIAPSRCKTNCHGDNLPDNTRRWQVSDEDEKAVIYLHDMSAQEKAGLNPG
                     WQCYTSFFMLACQGKPGTRPAVDENYVSKSGFFVSMLHIGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(22999..24639)
                     /gene="pgm"
                     /locus_tag="RHW33_001057"
     CDS             complement(22999..24639)
                     /gene="pgm"
                     /locus_tag="RHW33_001057"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV1644589.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGVSIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(24665..25210)
                     /gene="seqA"
                     /locus_tag="RHW33_001058"
     CDS             complement(24665..25210)
                     /gene="seqA"
                     /locus_tag="RHW33_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV1644590.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            25395..26159
                     /gene="ybfF"
                     /locus_tag="RHW33_001059"
     CDS             25395..26159
                     /gene="ybfF"
                     /locus_tag="RHW33_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1644591.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPTMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFSAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            26299..26592
                     /gene="ybfE"
                     /locus_tag="RHW33_001060"
     CDS             26299..26592
                     /gene="ybfE"
                     /locus_tag="RHW33_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV1644592.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            26732..27262
                     /gene="fldA"
                     /locus_tag="RHW33_001061"
     CDS             26732..27262
                     /gene="fldA"
                     /locus_tag="RHW33_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV1644593.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            27472..27558
                     /gene="uof"
                     /locus_tag="RHW33_001062"
     CDS             27472..27558
                     /gene="uof"
                     /locus_tag="RHW33_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV1644594.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            27551..27997
                     /gene="fur"
                     /locus_tag="RHW33_001063"
     CDS             27551..27997
                     /gene="fur"
                     /locus_tag="RHW33_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV1644595.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(28081..28407)
                     /locus_tag="RHW33_001064"
     CDS             complement(28081..28407)
                     /locus_tag="RHW33_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644596.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(28457..29863)
                     /gene="chiP"
                     /locus_tag="RHW33_001065"
     CDS             complement(28457..29863)
                     /gene="chiP"
                     /locus_tag="RHW33_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV1644597.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(30441..32105)
                     /gene="glnS"
                     /locus_tag="RHW33_001066"
     CDS             complement(30441..32105)
                     /gene="glnS"
                     /locus_tag="RHW33_001066"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV1644598.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(32308..34254)
                     /gene="nagE"
                     /locus_tag="RHW33_001067"
     CDS             complement(32308..34254)
                     /gene="nagE"
                     /locus_tag="RHW33_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV1644599.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            34587..35387
                     /gene="nagB"
                     /locus_tag="RHW33_001068"
     CDS             34587..35387
                     /gene="nagB"
                     /locus_tag="RHW33_001068"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV1644600.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            35447..36595
                     /gene="nagA"
                     /locus_tag="RHW33_001069"
     CDS             35447..36595
                     /gene="nagA"
                     /locus_tag="RHW33_001069"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV1644601.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     KNADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            36604..37824
                     /gene="nagC"
                     /locus_tag="RHW33_001070"
                     /gene_synonym="nagR"
     CDS             36604..37824
                     /gene="nagC"
                     /locus_tag="RHW33_001070"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV1644602.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            37872..38624
                     /gene="nagD"
                     /locus_tag="RHW33_001071"
     CDS             37872..38624
                     /gene="nagD"
                     /locus_tag="RHW33_001071"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV1644603.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            39021..40685
                     /gene="asnB"
                     /locus_tag="RHW33_001072"
     CDS             39021..40685
                     /gene="asnB"
                     /locus_tag="RHW33_001072"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV1644604.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDKA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            40995..41071
                     /locus_tag="RHW33_001073"
     tRNA            40995..41071
                     /locus_tag="RHW33_001073"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41029..41031,aa:Met,seq:cat)
     gene            41080..41164
                     /locus_tag="RHW33_001074"
     tRNA            41080..41164
                     /locus_tag="RHW33_001074"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41114..41116,aa:Leu,seq:tag)
     gene            41188..41262
                     /locus_tag="RHW33_001075"
     tRNA            41188..41262
                     /locus_tag="RHW33_001075"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41220..41222,aa:Gln,seq:ttg)
     gene            41297..41371
                     /locus_tag="RHW33_001076"
     tRNA            41297..41371
                     /locus_tag="RHW33_001076"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41329..41331,aa:Gln,seq:ttg)
     gene            41387..41463
                     /locus_tag="RHW33_001077"
     tRNA            41387..41463
                     /locus_tag="RHW33_001077"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41421..41423,aa:Met,seq:cat)
     gene            41511..41585
                     /locus_tag="RHW33_001078"
     tRNA            41511..41585
                     /locus_tag="RHW33_001078"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41543..41545,aa:Gln,seq:ctg)
     gene            41623..41697
                     /locus_tag="RHW33_001079"
     tRNA            41623..41697
                     /locus_tag="RHW33_001079"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41655..41657,aa:Gln,seq:ctg)
     gene            complement(41851..43026)
                     /gene="ubiF"
                     /locus_tag="RHW33_001080"
     CDS             complement(41851..43026)
                     /gene="ubiF"
                     /locus_tag="RHW33_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV1644605.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGVALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            43172..44596
                     /gene="miaB"
                     /locus_tag="RHW33_001081"
     CDS             43172..44596
                     /gene="miaB"
                     /locus_tag="RHW33_001081"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV1644606.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            44749..45789
                     /locus_tag="RHW33_001082"
     CDS             44749..45789
                     /locus_tag="RHW33_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV1644607.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            45786..46253
                     /gene="ybeY"
                     /locus_tag="RHW33_001083"
     CDS             45786..46253
                     /gene="ybeY"
                     /locus_tag="RHW33_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV1644608.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            46343..47221
                     /gene="corC"
                     /locus_tag="RHW33_001084"
     CDS             46343..47221
                     /gene="corC"
                     /locus_tag="RHW33_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV1644609.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            47246..48784
                     /gene="lnt"
                     /locus_tag="RHW33_001085"
     CDS             47246..48784
                     /gene="lnt"
                     /locus_tag="RHW33_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV1644610.1"
                     /translation="MAFASLIERQRIRLLLALLCGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            49181..50089
                     /gene="gltI"
                     /locus_tag="RHW33_001086"
     CDS             49181..50089
                     /gene="gltI"
                     /locus_tag="RHW33_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV1644611.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            50259..50999
                     /gene="gltJ"
                     /locus_tag="RHW33_001087"
     CDS             50259..50999
                     /gene="gltJ"
                     /locus_tag="RHW33_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV1644612.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            50999..51673
                     /gene="gltK"
                     /locus_tag="RHW33_001088"
     CDS             50999..51673
                     /gene="gltK"
                     /locus_tag="RHW33_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV1644613.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            51673..52398
                     /gene="gltL"
                     /locus_tag="RHW33_001089"
     CDS             51673..52398
                     /gene="gltL"
                     /locus_tag="RHW33_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV1644614.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            52516..53451
                     /gene="rihA"
                     /locus_tag="RHW33_001090"
     CDS             52516..53451
                     /gene="rihA"
                     /locus_tag="RHW33_001090"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV1644615.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            53535..55205
                     /gene="hscC"
                     /locus_tag="RHW33_001091"
     CDS             53535..55205
                     /gene="hscC"
                     /locus_tag="RHW33_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDV1644616.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFMQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGQVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(55265..56716)
                     /gene="djlC"
                     /locus_tag="RHW33_001092"
     CDS             complement(55265..56716)
                     /gene="djlC"
                     /locus_tag="RHW33_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDV1644617.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADNEDKDAAAEHEILRAFRALLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMDGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAQQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YWGSYPSTQADNLLLQWSAQHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(56713..>57417)
                     /locus_tag="RHW33_001093"
     CDS             complement(56713..>57417)
                     /locus_tag="RHW33_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV1644618.1"
                     /translation="NKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSSW
                     GINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFVA
                     QTAMCCGEGGIKAWDYVRMGFLSRVGVLNKWLTEDESLWLQSRVYVRAHHYYHSWMHY
                     FSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQPL
                     TVQPECPVTLKDVSDL"
BASE COUNT        13673 a        15056 c        14751 g        13937 t
ORIGIN      
        1 tatactttat catagcccat gaaggattca tgtatgttta aaggacaaaa aacactggcc
       61 gccctggcca tatctctact attcgcttca cctgtttttg ctgcagatga aggatctggt
      121 gaaatccact ttaaaggtga agttattgaa gcaccttgtg aaattcaccc tgatgacctt
      181 gatatgaaca ttgacctggg tgaagtaacc acatcccaca taaatcgaga acatcatagc
      241 gagaaaaaac ctgtcaatat tcgcttaatc aactgtgata ttgcaggctg ggatgatggc
      301 aagggtggcg tggtctcaaa ggtaggcgtg accttcgaca gcacggctaa aacgacaggt
      361 gcaacgccgc tccttagtaa catcagtgct ggcgaggcta ccggcgttgg cgtacgtctc
      421 atgaacaaag atgaatcatt tgttgtattg ggaacagaag cgccaacaat tgacttattg
      481 gcaagttcaa cagaacaaac gttaaacttc tttgcctgga tggaacagat ggataacgca
      541 gtttcagtta ccgcgggggc cgttactgct aacgcaactt acgttctgga ttaccaataa
      601 tcctttttaa ggcggctgat tagccgcctt ttgcgaatat tgttcaggta tggatgccgt
      661 gaatatttat cgactctctg ttctttcctg tctggcaatg gtaacccctc ctgcgctggc
      721 agctgaattt aaccttaacg ttctcgataa gtcgattcgg gatagcgttg atatttcgtt
      781 gctcaatcaa aaaggcgtcg tagctcccgg cgattacttt gttagtgtta cggttaataa
      841 taataaaatc agtaatgggc aacaaattcg ctggcaaaaa tctggcgata aaattattcc
      901 ttgtatcaat gaatcgctga tcgaactctt cggacttaaa tctgactttc gtaaaaaatt
      961 accggcaata aaagaatgtg tcgattttag tgtcttccct gaaatcatct ttactttcga
     1021 tcaggcaaat caacagctca atattacaat cccgcaggca tggctggcgt ggcattcaga
     1081 aaactggacg ccaccctcaa cgtggaataa cggtattcct ggcttcctga tggattacaa
     1141 cctgtttgcc agcacttatc gcccacaaag cggcagcagc agcaacaacc tgaacgccta
     1201 tggcaccacg ggcctcaacg ccggggcatg gcgtctgcgc agcgattatc agcttagcca
     1261 gtccgatagc ggcgacaacc gtgaacaatc cggggcgatt tcacggacct acctgttccg
     1321 cccgttaccg caaataggtt cccgattaac cctgggcgaa accgatttta gctctaacat
     1381 ttttgatggt ttctcctata ccggtgcggc gttagccagc gacgatcgaa tgctcccctg
     1441 ggagttacgc ggctacgcgc cgcaaatcag cggcatcgca cagactaacg ccacggtcac
     1501 gataagccat tctggtcgcg tgatttacca gaaaaaagtt ccgccaggcc catttattat
     1561 tgatgacctg aatcagtcag tgcaaggttc actggatgtc aaagtgagcg aagaagatgg
     1621 acgggtgaat aacttccagg tttcagcggc ctctaccccg ttcctgacgc gtcagggcca
     1681 ggtgcgctat aaactggctg ccggtcgccc gcgttcctct atgtcgcacc acaccgagga
     1741 tgaaaccttc attagccatg aagtctcctg ggggatgctc tccaacactt cactatatgg
     1801 cggcatgctt ctcgcaggcg atgactatcg ttccggcgcg ctgggtattg ggcaaaatat
     1861 gctctggctg ggggcgctgt cgtttgacgt gacctgggcc gacagccatt ttgatatcca
     1921 acaggatgag cagggttaca gctaccgctt taactacagc aaacaggttg atgcgaccaa
     1981 cagcaccatc tcattggcag cctatcgctt ctccgatcgc cattttcaca gctacgctaa
     2041 ctacattgat cacaaataca atgacgccga cgcgcaggat gaaaagcaaa ccatcagcct
     2101 ctccttcggt cagcctatta ctccgctgaa tcttaatctc tacgccaaca tcctgcatca
     2161 gagctggtgg aatgcggata cctccaccac ggccaacatt accgccggtt ttaacgtcga
     2221 cattggcgac tggaaagata tttctgtctc gacctcgttc aacaccaccc attatgaaga
     2281 taaagatcgc gacaaccaga tttacttttc gatctcctta ccgattgggg aaagtggtcg
     2341 actgggttat gacatgcaga acaacagtaa tactaccacc caccgcatgt cgtggaacga
     2401 taccctggat gaacgaaaca gttggggaat gtcggctgga atacaatccg atcgcccgga
     2461 taacggtgcg caggtcagcg gcaattacca acacctcagt tcggcggggg aatgggatct
     2521 gaccggaacc tatgccgcca atgattacac ttccgccagt gccagttgga gcggctcgtt
     2581 taccgcgact caacatggcg cggcattcca tcgccgcagt tccaccaatg aaccacgcct
     2641 gatggtcagc accgacggcg tgggcgatat tccgattcag ggcaatattg actacaccaa
     2701 ccgctttggt attgccgtgg taccgtttgt ttccagctac cagcctacga cggtggcggt
     2761 caatatgaac gacttgcctg acggcgttac cgtttccgaa aacgtggtga aagaaacctg
     2821 gaccgaaggc gccatcggct ttaaatcgct ggcgtcccgc gccgggaaag atctcaacgt
     2881 cattatcagc gatgctaacg ggcatttccc gccgctcggt gccgatgttc gccaggctga
     2941 aggcggtgtc agcgtcggga tggttggcga aaacggtcac gcatggctga gtggggtcga
     3001 tgaaaatcaa caatttaccg tgcactgggg cgaccagaaa acgtgtgcca ttcatctgcc
     3061 ggaacatctg gaagatgtga ctaagcgcct gattttaccc tgtcattaat taaggaaaag
     3121 acgatgacat ttgttaaagg attccctttg attcttctgg ttgccagtat gtgcagccac
     3181 ggcgcagtgc agcctgaccg cacgcgcatc atctttaata gcaaagataa agcaaccagc
     3241 ctgcgcgtgg aaaaccgcag cgataaactc ccctacctcg cctattcatg gatagaaaat
     3301 gaaaagggag agaaaagcga tgaatttctc gtcgccctgc cgcctatcca gcgactggag
     3361 ccaaaagcga cttcgcaggt tcggatcatg aaacaagccg ccaccgcgaa attacccacc
     3421 gatcgcgaat ctctctttta ctataacctg cgtgaaattc cgcctgtccc ggaaggcagc
     3481 gagggccatg cgatcctgca agtcgccgtc caaagtcgca ttaagctatt ctggcgaccc
     3541 gccgcattac gcaaaaaaaa tgggcgatca tgttgagcaa cagctacagg ttagccagca
     3601 gaataaccag cttaccctga aaaacccgac cggatactat ctgactattg cctatcttgg
     3661 gcgggatgag aaaggcgtgc tgcctggctt taaaagcacg atggtcgcac cgttcagttc
     3721 ggtcaccacc agcacgggca gctatagcgg caagcagttt tatcttggct acatggacga
     3781 ctacggcgca ttgcgtatga acacgctctc ctgccagcgg caatgccgcc tgcagccggt
     3841 ggagaataaa aaatgagatt cgccaaaggc gtgttgctcg ccatctgtct gatttttttg
     3901 ccgctaaaag cggcgctggc gctgaactgc tattttggta ccgcaaacgg cgctgtagaa
     3961 aaatcggaag ctattatgcc cttcgccgtt ccggccaatt ccaaacccgg cgataaaatc
     4021 tgggaatctg acgatataaa aattccggtc tactgtgaca acaacacaaa cggtaatttt
     4081 gaaagcgagc acgtctacgc ctgggtaaac ccttaccccg gtattcagga tccctattat
     4141 caattaggcg taacctatga aggcgtggac tatgacgcaa gccttgggaa aagccgcatc
     4201 gacacgaatc agtgcatcga cagtaagaat attgatattt acaccccgga gcagatcatt
     4261 gcgatgggct ggcaaaataa actttgctcc ggcgatccaa ccgttatgca caaatcgcgg
     4321 acgtttgtgg cgcgtatgcg gctttacgtt aaagttcgcg ccatgccgcc ccatgattat
     4381 cagagcaaac tcagcgatta catcgtcgta cagtttgatg gcgcaggcag cgtgaacgaa
     4441 gaccctaccg cgaagaatct gaaatatcac attaccggtc tggaaaatat ccgcgtgctg
     4501 gattgcagcg tcaactttgc tatctcacct gaaacccagg tcgttgattt tggccgattt
     4561 aacgtgctgg atattcgtcg ccatacgatg tcgcagcaat ttaaaataac tacaaccaaa
     4621 tcacaaaacg accagtgtac cgacggtttt aaagtcagct cttcgtttta taccgatgaa
     4681 acccttatcg acgaagataa atcactgctc attggcaatg gcctgaagct tcgattgttg
     4741 gatgaaaatg cctcacccta tactttcaac aaatacagtg agtatgccga ttttaccagc
     4801 gacctgttag tgtatgaaaa aacctacacg gcagaattgt catccacgcc gggtacgccc
     4861 atcgacgttg gtccctttga tacagtggta cttttcaaaa ttaactacaa ctgattttgc
     4921 ctattgccct ttccagccgg agagggcttc ttcggttaat tgccaatttt ccggctttta
     4981 ttgattaccg ttattttttc catgcccggc attctttatg ctgtatcgat acaccaaaga
     5041 gtaaataatg agggagcaaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc
     5101 acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcattat tacttgggcc
     5161 gatgatcgcc gggatcatct tcagtatgcg cggggttacc ctgcaactcc cccgctccgc
     5221 ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacttga ccggttcgat
     5281 cctgaccacg ctggcggtta actggccgat tgtgttagct attttactgg tgacgttgct
     5341 ttccagcgct atcgtgggat ggctactggt gcgctatagc tcactgcccg gaaatactgg
     5401 tgcctggggc tcctcgcctg gcggggcggc ggcaatggtc gcgatggcgc aggattacgg
     5461 cgcagatatc cgcctggtgg cgtttatgca gtatctgcga gtgctgtttg tcgcgggggc
     5521 cgcagttctg gtcacgcgaa tgatgctggg cgataacgct gaagcggtta accagcatat
     5581 cgtctggttc ccgccagtga gcattaatct cctgcttact attttgctcg cggtcgttgc
     5641 cggtacagtg ggatgcctgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc
     5701 gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc
     5761 gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact
     5821 gcgggcacta cgcccgctac cacaaatcct gctgtcgatt tttgctctgc tggctatttg
     5881 tgcgggtatg gcgtggggac tgacccggtt tatgcacatt gattttatga ctgcctacct
     5941 cgccaccagc cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga
     6001 tatggcgctc attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacagggcc
     6061 tgccattgcg cggtttattt caacctatgc gccgaaacgt acggcctagt gctggcagcc
     6121 cggacaccag taaaacgggc gagatgacag cgtggttttc tcaatgatgc cgccacagcg
     6181 ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt
     6241 attctcatcc acctgccccc gcgtagcgta agaaaaccga ggaatatcca gtaacgcgtg
     6301 tgcgagtgca tccagttgcg ccgcattgag gtctttcgct ttatgatttc cagtcaaccc
     6361 aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc
     6421 gagcagtaat ccggcaaact gacggttacg aaagcgcggc gacaataacc gttctttcac
     6481 cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg
     6541 cgtggtcagt tgttccgggg tcaacatctc aatatccgag gcgctataaa gcagaatggt
     6601 tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcgact cttcgccggt
     6661 atcaaccacg cgccagacgc cataaagctg attatggctg tagagcgtta agtcgttgga
     6721 aaaatgagtt aacagcgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag
     6781 ttgtgattga taagatttta actgcgggaa agcaaaccag acatcagtta gtggtttgcc
     6841 tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt
     6901 gatatccttt tttcattctt tattaaacgg ggttatgctg cgacaacaat ccccttttcg
     6961 gcaaatgtag agcgtagtcg gcgggcgaac gccagtgcgt gctcgccgtc gccatgcagg
     7021 cagaccgttt gcgccgtgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt
     7081 tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa cgcgcctggc
     7141 tgatttcgcg gcaccagcaa gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg
     7201 cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccgacg
     7261 agaatcaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct
     7321 tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct
     7381 tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt
     7441 tctggcggca gctgcatagc gctgcgacca aaattttccc tgtcgggaaa actcggatga
     7501 gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat ggtttgcgca
     7561 tcgcctgcat gaaatccaca ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg
     7621 tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca
     7681 cgctaattgt tcgaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga
     7741 acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt acatatcagc
     7801 ctcaatgata caggcaatac gcgggtaacc accggtggtc tgtgcgtcgt tcatcaacac
     7861 aatgggctgc ccgttatgcg gcacctgcac cacacccggt aacaaaccgt gagataacag
     7921 ttcgcgatcg gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca tgcggttact
     7981 ttgcgggcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc gatcgaactc
     8041 atgatattcc ggccccggca aggcgcgaat gcggttcccc cacagcagct gtttaacgcc
     8101 ctgcgcttcc ataaaatcac gcttcgcttt gccaatcggg agccggtcac catccctcag
     8161 taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg atcccattac
     8221 cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg
     8281 tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcatt
     8341 atcatccagc cgcgcttcgc aaccggcacc cgtcagagca aaccacccat cagtttcgaa
     8401 ctcaaccgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac
     8461 cagcaggtta gcaatgcgta acgcaggcat atccagtgcg ccgcagtggc tgatacccga
     8521 ctggcgaaaa ccgtgacgac cgccatcttg cacagtggta tacatgcccg cacgaataat
     8581 cttcagcata ctccctcctt ttgcggtaca aagcgcacgc tgtctcccgg acgtaataag
     8641 atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag
     8701 ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct
     8761 gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc
     8821 ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag
     8881 tgcaattcaa caacctgttt ttcgctcaac ccgcaatgcg ccgcgaccac cgccaaatcc
     8941 ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg
     9001 gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca
     9061 ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac cacattcggc
     9121 atatccacca gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc
     9181 ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact
     9241 ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag
     9301 atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccacgtt cagtggcatg
     9361 gtgacctgca gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg tctgttcaga
     9421 aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg
     9481 cccgccgccc gtgcaccagg cgacgcgctg gaccacctca ggtccggtat cgccacacca
     9541 taacggctta cgtcccagac gcgcttcaat ccaggaagcc agctccagtc ccggcacagg
     9601 catggtcagt tcgccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag
     9661 taacgccgcc agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg gcaaatgcca
     9721 gccatacagg ttgatatcat ttgccagcaa cgtttttaaa cggttacgct tcattccgcg
     9781 aatgaccgga gattcacctt tccagaagta gccatgatgc acaatgaccg catccgcgcc
     9841 cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg
     9901 caccgtctct ttgccttcca cctgcaaacc atttggcgcg taatcactaa tcgccgcgct
     9961 gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt
    10021 attctgatag ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat agccaatctt
    10081 ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgtt
    10141 tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc
    10201 aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgccgtc
    10261 aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcgctattta ctatgtcgtt
    10321 gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat
    10381 ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt
    10441 tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat
    10501 cgcatggcgg tgatgctggg ggcgttattg atggcgatcg gtcatgtggt gttgggtgca
    10561 agtgagatcc atccgtcatt cctctatctg tccctggcga taatcgtttg tggctatggt
    10621 ctgtttaaat ccaatgtcag ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt
    10681 cgtgatggcg gtttctcgct gatgtatgca gcgggtaacg tggggtctat tatcgcacct
    10741 attgcctgtg gctacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg
    10801 gttggcatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cactcatacc
    10861 cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc tgctgccgaa ctggggatgg
    10921 ctgctggttc tgctggtggc aacgcctgcg ctgattaccg tactgttctg gaaagaatgg
    10981 tcggtatacg ccttgattgt cgcgactatc attggcctgg gtgtactggc aaaaatttat
    11041 cgcaaagcag aaaaccagaa acagcgtaag gagctggggc tgattgtgac gctcaccttc
    11101 ttcagtatgt tgttctgggc cttcgcacaa cagggcggta gctcgattag cctttatatc
    11161 gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgaccgcgat gttccagtcg
    11221 attaatgcct tcgcagttat gctgtgcggt gtgttccttg cgtgggtggt aaaagagagt
    11281 gtcgcgggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct tggcctgatg
    11341 agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct
    11401 tctctgccac tgatggtatt aggcctggcg gtgatgggct ttgcggaact gtttatcgac
    11461 ccggttgcta tgtcgcagat tacgcgcatt gaaatccccg gtgtgaccgg cgtattaacc
    11521 ggcatttata tgctgctttc cggcgcgatt gcgaactatc tggcgggcgt gattgccgat
    11581 cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat
    11641 attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggagt ggtactgatg
    11701 atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcttaatag
    11761 gccagcaccg atgcctgatg cgacgcttga cgcgtcttat caggcctacg tatttcctgc
    11821 aatttattga atttgcacaa atttgtaggc tggataaggc gttcacgccg catccggcat
    11881 ctgggctcga ttgcctgacg cgtctgttat ttccccttcc gcgccgcctc atacgctgcc
    11941 aacgtttgta ctctcgcctc tttgtgctcg actatcggtt gcggataatc cagcttcaca
    12001 cctgctttct cagcccactt ccacggctca tgcaccgatt ttcccggtac atcgcgcagt
    12061 tccggtagcc actggcggat aaactcgcct tcgcggtcaa atttctcgcc ctgggttgtc
    12121 gggttgaaaa tacgaaaata cggcgctgca tcagtacccg tagaagccgc ccactgccag
    12181 ccaccgttat tagctgccaa atcaccatca atcagctgcg acatgaaata tcgctcgcct
    12241 tcgcgccagt cgatcaataa atctttcacc agaaaactgg ctgtaatcat ccgtagcctg
    12301 ttatgcatcc agccagtgct gttaagctga cgcatggcag catcgacaat cgggtatccc
    12361 gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc tctgccactg cacacgatcc
    12421 gtccaggcaa taaacggaca atgtttacac aacgaggggt aatacgtcat cagatgacgg
    12481 tagaactcgc gccagatcag ctcactaagc cagacactac cggccccacc gtccagcgcc
    12541 tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc gcccgttgcc
    12601 aggctggcgg acaaacgact ggtgccttcc actgccggaa aatctcgttg ttgctcatat
    12661 tctccggcac cgttctggca aaactggcgt aattgcgcaa tcgccgcttt ttcttccacc
    12721 gggaaatgcg cagtatcgaa agactgacga ggataattca gcgtaatgga tggcgcgggc
    12781 tttatcgatc cgctactacg aacttttggt gcagcgacgc actccggcat cccttcccgc
    12841 agccgtttca gccaagcatt cttaaaaggc gtaaagactt tgtacatctc gtgattgcca
    12901 gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa atccttcaca caccacgtta
    12961 cgcagcgctc tttcaacgtc cacatcccgc gcccgctcat tcacctcata ctgatagtta
    13021 taaaaaagat gagtaacgct gttttccgcg cacacctgtt taactaattc gacgctggcg
    13081 acaaagtcat ccacttcacg gaacaataaa ggaatacctt tttccgccag cgcgatttgt
    13141 agcccgttca gttgagcatt gataagttca gcctgacgcg gcgacatatt atgcgccgcc
    13201 cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg cagacgaatt gcggcaggcg
    13261 gcagccagcg cgagattatc gtgcagacgt aaatcctggc gaaaccagac cagatgggta
    13321 gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg ccaaagttcg aaataacgct
    13381 gccctgaaag ccaggcgtca ggataaagcg ccatatagtg tttgatacgc atcagcggcg
    13441 caagaagcgg ttgctcgcca cggcgataac tgtcgataag cgctgccagc tgctggcgtt
    13501 ctgtggaatt aatatgcggg cgaaaataac cctgaacgtg catcaaaacg ttggtgtggt
    13561 cacgtcggtt ggcggggtga gaaagcaaaa caactacgcg ttggcggtac tggttgtaat
    13621 agtcatccag attttgccac tggtgaatat cagccacgaa cgggccaagt ttgcggtatt
    13681 ccggctggga atgagccaga aagacgagtt tatagcggct gtgataatcg agaagcgccc
    13741 cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg aagggcgaag atacggatta
    13801 gggtgctgtc atcaaatcgt tgctgattca ttactgctcc tgaacggttt acaggagtaa
    13861 gtgtagacgg cgaaaagaaa aaaccgccag cgtttccact agcggttttc aatgtattca
    13921 aaactcagca atcagtaaaa atcaccagtt gccttttcgg cctgatccat ccacaccggt
    13981 ttatcgctgg ttttcgacca gacgcgatgc aggtagctgt aaaagcgggc gcggtctttc
    14041 cagaaaagca tgaaaggcag cgccagaacg cccgctgcaa ccgcataagt acggcgtaaa
    14101 aagataagcc atgcaggata ttctctgtag agttccatac ttttctcccc cttttgtatc
    14161 tacccggtga atggcaccgg aaaatgaatt tgtttatctg atgaaaatag taccgccttt
    14221 tgtgtaattt tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt
    14281 caccgtgtaa ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc
    14341 ttttgccatt tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag
    14401 ccagaattct acccttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg
    14461 cgtgataacc ggcgtattgc tggtgttttt attactgggt tatctggttt atgccctgat
    14521 caatgcggag gcgttctgat ggctgcgcaa gggttcttac tgatcgccac gtttttactg
    14581 gtgttaatgg tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt
    14641 cctcttccag gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac
    14701 catgagatga actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg
    14761 gcggtgctgt tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg
    14821 ccggggctgt cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac
    14881 tggcaatctt atagcggtga aaccacgttg agctatttca gccagatggc gggcttaacg
    14941 gtgcaaaact ttctttctgc cgccagcggg attgcggtga tttttgccct cattcgtgca
    15001 tttacccgcc agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg
    15061 ttatgggtgc tcgtccctgt ggcgttggtg atcgcactgt tttttattca acaaggtgcg
    15121 ctgcaaaact ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta
    15181 ccaatgggtc cggtggcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc
    15241 ttctttaatg ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg
    15301 cagatgctgg cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg
    15361 ggcgatcgcc gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc
    15421 gtaggcgtgg tgatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca
    15481 gacagcagca tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg
    15541 tttgcggtcg tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt
    15601 accgctctcg gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt ggtattcggc
    15661 ggtgtcggtt ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc
    15721 gggctgatga ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgtgagatg
    15781 aaactgactg cactggcgat tctggtcacc ccgacgctgg tgctgatggg cgcggcgctg
    15841 gcaatgatga ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca tggttttagc
    15901 gaagtgctgt acgccgtgtc atccgccgct aacaacaacg gcagcgcctt tgccggatta
    15961 agcgccaact ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc
    16021 ggagtgatta tcccggtgat ggcaattgcc ggttcgctgg taagtaaaaa gagccaaccc
    16081 gccagctccg gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc
    16141 gtgttgctgg ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa
    16201 tatctctcct gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt
    16261 cgaaccaaca cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc
    16321 gcaatggcgc aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat
    16381 tagcatcgcg atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag
    16441 cggctggctg tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg
    16501 cagtaaagcg caggccaaca gtctgaaagg ggtgaaaaaa accgcttttg ctcgcaagct
    16561 gcgcgaaccg aaatatggcg ctgtggcaga caaagttcct gccgatcaac tgcgtaaagg
    16621 cgatatcgta ctggtagaag ctggcgatat tatcccctgc gatggtgaag ttattgaagg
    16681 gggtgcatcg gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc
    16741 cggcggcgat tttgcctccg tcactggcgg cacgcgtatt ctttctgact ggctggtgat
    16801 tgagtgtagc gttaaccccg gcgagacatt tctggatcgg atgatcgcga tggtggaagg
    16861 cgcacagcga cgcaaaaccc cgaacgagat tgccctgacc atcctgctga ttgccctgac
    16921 tatcgtcttt ttactggcaa ccgccacgct gtggccgttt tctgcgtggg gcggtaatgc
    16981 agtcagtgta acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg
    17041 cctgttgtca gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc
    17101 caccagcgga cgtgcagttg aagcggcggg tgacgttgac gttctgctgc tggataaaac
    17161 cggcaccatc acactcggta accgtcaggc atcggagttt atccctgcgc agggcgtgga
    17221 tgaaaaaacg ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg
    17281 ccgcagtatt gtgatcctcg ccaagcagcg ttttaacctg cgcgagcgcg atgtgcagtc
    17341 gctccatgcc acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga
    17401 caaccgcatg atccgtaaag gttctgtcga tgccattcgc cgccatgttg aggctaacgg
    17461 tggtcacttc cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggggccac
    17521 accgctggta gtagtagaag gttctcgtgt gctgggcgtt attgcgttga aagatatcgt
    17581 caaaggcggc attaaagaac gcttcgccca actgcgcaaa atgggtatta aaacggtgat
    17641 gattaccggc gataaccgtc tgactgccgc cgccattgcc gcggaagcgg gcgtcgatga
    17701 ttttctcgcc gaagcgacac cggaggccaa gctggcattg atccgtcagt atcaggcgga
    17761 aggccgtttg gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc
    17821 agatgtcgcg gtggcgatga actctggtac tcaggcggcg aaagaagcgg gcaatatggt
    17881 ggatctcgac tctaacccga ccaagttgat cgaggtggtg catattggca aacagatgct
    17941 gatgacccgt ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc
    18001 cattattccg gcggcattcg cggtaacgta tccacagtta aatgcgctga acatcatgcg
    18061 cctgcattcg cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt
    18121 ctttttgatt cccctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat
    18181 gttgcgccgt aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat
    18241 caaagtcatt gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgcgtgga
    18301 ttacgtccgg cattatcgac atttatcttt ctgttattga ttactggcgg tgtttacccg
    18361 ctgctgacca ccgcgctggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc
    18421 cgtgaaggtg atacggtgcg gggttcggca ttaatcgggc agaattttac cggcaacggc
    18481 tattttcatg gtcgcccgtc ggcaacggca gaaatgcctt ataatccaca ggcttctggc
    18541 gggagcaatc tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct
    18601 gcattacggg ccgctaaccc gaatgccagc acgagcgttc cggttgaact ggtgacggcg
    18661 tcggcaagcg ggctggacaa taacatcacc ccgcaagcgg cggcctggca gatcccacgc
    18721 gtagcgaaag cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc aaaatacagc
    18781 caacaaccgc tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg
    18841 gcgctggata aacttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga
    18901 acaaactgcc gcgccgcatc gggggaagct gaaagttttt ttcggtgcct gcgcaggcgt
    18961 cgggaagacc tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat
    19021 tgtggttggc gtggtagaaa cccacgggcg gaaagatacc gccgcaatgc tggaagggct
    19081 ggctgttctg ccgccaaaac gcctggcgta ccgtgggcgg catatcagcg agtttgatct
    19141 cgatgccgcc ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa
    19201 tgcgcctggt tctcgtcatc ccaaacgctg gcaggatatt gaagagctgc tggaagcagg
    19261 cattgatgtt ttcacaaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag
    19321 cggcgtcacc ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga
    19381 cgacgtggtg ctggttgact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa
    19441 agtctatatt gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct
    19501 gatcgccctg cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg
    19561 cgcctggcgg gggcatcctg gcgaagagaa agtctggcac acgcgcgacg cgatcctttt
    19621 atgcatcggc cataacaccg gcagcgaaaa actggtccgc gcggcggcgc ggctggcgtc
    19681 acggctgggg agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc
    19741 ggagaaaaaa cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga
    19801 gacggcaaca ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca
    19861 taatctcggc aagattattc tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac
    19921 gtttgctgac cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga
    19981 tgaaccaccc gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg
    20041 gcgggtacaa attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat
    20101 tgccatgcag tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctacttgg
    20161 cgtggtggtg gtggcgctat tttatggacg ctggccttcg gtggttgcta ccgtcattaa
    20221 tgtggtgagt ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt
    20281 gcaatatctg ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac
    20341 tgcgggtgtg cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt
    20401 atatgaaatg tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag
    20461 cgaacaattt attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa
    20521 cggtaaattg cagccgttaa cacatccgca aggaatgacg ccgtgggacg atgccatcgc
    20581 gcagtggagt tttgataaag gcctgcctgc gggtgcgggc accgacacgt tacccggcgt
    20641 gccgtaccag attttgccgt taaaaagcgg tgagaaaacc tacgggctgg tggtggtgga
    20701 accggggaat ctgcgtcagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac
    20761 gctgttagtc gccaatgcgc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg
    20821 gatggcaagc gaacgtgaac agatccgcaa cgccctgctg gcggcgcttt cgcatgattt
    20881 acgcactccg cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag
    20941 cgaaggatca ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac
    21001 ccgactggtg aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa
    21061 gaaagagtgg ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg
    21121 tttatcgtcg cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg
    21181 gccactcttt gaacgggtgc tgattaattt gctggagaac gcagtgaaat atgcgggtgt
    21241 gcaggccgaa attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg
    21301 ggataacggc cccggacttc cgcaaggcca ggagcagtcg atttttgata agtttgctcg
    21361 tgggaataaa gagtcggcag tcccgggtgt agggcttggg ctggcaattt gtcgggcgat
    21421 tgtggatgta cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt
    21481 tcgtgttaca cttccccagc aaaccgctcc tgaacttgaa gaatttcatg aggatatgtg
    21541 acaaacgttc tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg
    21601 gagggcgacg ggatgcgcgt ctttgaggcc gaaacgctgc aacgcggctt gctggaagcg
    21661 gcaacccgta agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt
    21721 gagtttatcc gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc
    21781 agcgaagaga gcgacaaaat cgccgcgctg gatgccggag cggatgatta tctgagtaag
    21841 ccgtttggca ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc
    21901 accgctgcgc ccgatccgct ggtgaaattc tccgatgtta ccgtcgattt agccgcccgc
    21961 gtgattcacc ggggtgagga agaggtgcat ctcaccccga ttgaattccg cctgctggcg
    22021 gtgctgctca acaatgccgg aaaagtgctc acccagcgcc agctccttaa ccaggtatgg
    22081 ggaccaaacg cggtcgaaca cagtcattat ttgcggattt atatggggca tctgcgacaa
    22141 aaactggagc aggatcccgc ccgccctcgc cattttatta ccgaaaccgg tattgggtat
    22201 cggtttatgc tttgaggggc tgaattgccg gatgcggcgt gagcgcctta tccgacctac
    22261 acatcgtccc gtaggcatga taagacgcgt cagcgtcgca tcaggcatgg cactcatcgc
    22321 gaaatgttta atgcgtattt atcgctttca attcttgctg gcaattttct gactgacagc
    22381 gtcgcaattc aataatcccg atatgtaaca tcgagacaaa aaatccgctt ttgctgacat
    22441 aattctcatc aacagccggt cgggtgccgg gcttcccctg gcaagccagc ataaaaaatg
    22501 aggtgtagca ttgccagccg ggatttaaac cggccttttc ttgtgctgac atatcgtgaa
    22561 gataaatcac cgctttttcg tcctcatctg acacctgcca gcgccgggta ttatcaggta
    22621 ggttatcgcc atggcaattt gtcttacacc gggaaggcgc aatttttaaa acagcgctac
    22681 cttctaatac ctgcttttta tccacatgcg tcggtaacca gtcgccagct aatgatgatt
    22741 gttcaggttg ggcgcatgca gtcaacagta atgtggcggc aatgaatgcc actcttacga
    22801 ccagactcct tttggtcttc attttgaatt cccttgagct attcagtttg ttggcaaagt
    22861 gcgcgttgtt tttgccggat gcggcgtaaa cgccttatcc ggcctacgaa atcatacaaa
    22921 ttcaatatat tgcaccatca ggcagtcata cataaaaaag ggcgatcttc cgaccgccct
    22981 ttttcttatt taatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc
    23041 tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga tcttatatgc
    23101 gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc
    23161 gccaatagaa acaccgttgc ccggagcagc agtcaggcgc gcggtgatcg ggtcaccagc
    23221 cagggtgctg gcgctcacca tttccggaga cagcttagac agcgccgctt tttgtgcgga
    23281 agtcgcagct gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt
    23341 gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat
    23401 gatgatgccg tctttgtcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc
    23461 tgcgctctct tcgccgccaa agccgaagct gccgtcgaac agaccatcaa caaaccattt
    23521 gaaacctacc gggacttcta ccagcttacg acccaagtca ttgaccacac ggtcgatcat
    23581 cgcagaagaa accagcgttt taccaacggc aacatctttg ccccactgcg gacgatgctg
    23641 gaacaggtag ttgattgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt
    23701 gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg
    23761 cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga tcgcgccgtc
    23821 tttatcaagg tgcataaagc ggaaagtttg atcgacctga tcgttaacga tggtcaggtt
    23881 gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc ccagcggatc
    23941 aacgccaagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagccc
    24001 ttccacgaac ggctgcacca gatcctgctc tttcacatga ccggatgcca tcgcttcgtc
    24061 gagggagata cgcttcacgc ctttcaggcc atcggccagc agtgcgttgg cacgatcttc
    24121 caccacttta gtgacgttgg tatctgccgg gccaccattt ggcggattgt acttgatacc
    24181 accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc
    24241 ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggggtga agccattgtt
    24301 ttcctgcaca ataacatcta cgccgttcgc tgccagcact tccagaacgg aaataaatgc
    24361 aggttcggac agggcgtgag tatctttacc cacatagcaa gggccagtga tgccgttttt
    24421 cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg
    24481 gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc
    24541 tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact
    24601 ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa
    24661 cgttttagat agttccgcaa accttctcaa tcaattccgc cgggaattgc atcgactgca
    24721 tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg
    24781 gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat
    24841 ctgccgcaaa gtaaacgcgc gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct
    24901 gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt
    24961 gctctgcgta ttcatccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg
    25021 ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg
    25081 gagcagcagg ctgtgatgcg gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc
    25141 tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa
    25201 tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg
    25261 aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct
    25321 tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa
    25381 aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata
    25441 attctcccat cgttcttgtc catggtctgt ttggcagcct cgacaacctt ggcgtactgg
    25501 ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt
    25561 caccgagaga tccggtaatg aattacccga cgatggcgca ggatcttgtt gataccctgg
    25621 atgcactgca gatcgacaaa gcaacattta tcggtcactc catgggcggt aaagcggtaa
    25681 tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc gatatcgcac
    25741 cggtcgacta tcacgtacgc cgtcatgatg agattttctc ggctatcaac gcggtcagtg
    25801 aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag
    25861 aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc
    25921 cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc
    25981 accctgccct gtttatccct ggtggtaatt ctccgtatgt tagcgagcag taccgtgatg
    26041 atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg
    26101 tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac
    26161 gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc
    26221 atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc
    26281 cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc
    26341 gcgcacgagc gtcgaccggg acgaccgaaa acaaatccgc tttcgcgcga tgaacagctg
    26401 cgtattaata aacgcaacca actaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa
    26461 ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggaatc gcgcaatatg
    26521 agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag
    26581 ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat
    26641 tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca
    26701 tttcaataag tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag
    26761 cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt
    26821 tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt atgacattct
    26881 gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt
    26941 cccgactctc gaagagattg atttcaatgg caaactggtt gcgctgtttg gttgtggtga
    27001 ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga
    27061 accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc
    27121 aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc
    27181 ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct
    27241 cgacgaaatt ctcaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat
    27301 agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg tacctgtaca
    27361 atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta
    27421 acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc
    27481 attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt agtaacagga
    27541 cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct
    27601 tccacgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga
    27661 agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg
    27721 cgtactgaac cagtttgacg acgcgggtat cgtcacccgc cacaattttg aaggcggtaa
    27781 atccgtattt gaactgacac agcaacatca ccacgatcac ctgatctgcc tcgactgcgg
    27841 caaggttatc gaatttagtg atgattcaat cgaagcgcgt cagcgtgaaa ttgccgcaaa
    27901 acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga
    27961 ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agtctgaacg agaaaagcca
    28021 acctgcgggt tggctttttt atgcaaggga aataagaaaa gcgagagtta cagctctcac
    28081 ttatttgtta ttactgcgaa tttcctgcca gaccttatca caatcggctt tcaccgcctg
    28141 atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc ggacactttc
    28201 ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca
    28261 ggcttcaatt ttttccggcg aaccttgtgc ggtgttgata caggcgctgt atgcctcttt
    28321 cagacggctg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc ccgatgccat
    28381 catggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc aggcacaaaa
    28441 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
    28501 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt
    28561 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
    28621 agaccgcatc caggctgtat gcagactctt caatagtccg gtttttgtcg tagtacgcat
    28681 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
    28741 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
    28801 tttcgccgtt ggcgttgaag tcagaacggt tatcccacca gatatccagg cgaccgttcg
    28861 aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg tcagctttaa
    28921 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
    28981 cttgcagcca ggcggtgccg tcataaagat cgttgacgct gcgatcgtca actttatcgc
    29041 gcgtaccgta gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
    29101 tggctttggc aaagtattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
    29161 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg cagataatca actttggtag
    29221 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggtgctttg tattcgttgg
    29281 tccacatgta ggagaaactc aacgcaccag catcgccgta atcaaaattc gctccggctt
    29341 ccgcaccctg ataagtaccc ggcataaagc tccagtgagg cgctaacagc gtctgaccgg
    29401 ttggctgaat ataacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
    29461 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
    29521 ttgaaaacgc gatttcgttc gggtgagagc tgtcgccgtt ttccgccatt tcaatcgccg
    29581 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt
    29641 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
    29701 tacggtcgcg ttcacgctgc cagtaataga taccgccggt taaggttgaa tcgtcgatga
    29761 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga
    29821 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
    29881 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata aaaattaaaa aagtagcgtt
    29941 gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat tatcaatctc
    30001 tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga tttttgttac
    30061 aaggcttaag tttgcggttg aaaggcttgc agaagaataa aagcgtgaaa ttccagcaag
    30121 atgaaagcgg ttacatttta tttaattatg ttttgtgatt ttaaatttat tttatggata
    30181 aattgcgaag cggaattaat tcgcgaagaa aagcagaaaa aacgccgctg aacgcggcgt
    30241 ttgggattgt ttgcatattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    30301 agcacgcaaa ctcaatatat tgcagagatc atgtaggcct gataagcgta gcgcatcagg
    30361 caatttagcg tttgaaactg tttgtctgtt ccttgccgga tgtggcgtaa atgcctcacc
    30421 cggcctggta gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg
    30481 gtgcggttaa ataccggttt ttccgccgtg gaatggcgac tgtcgaggca gaagtaacct
    30541 tcacgctcaa actggaatgc tttacccgct accgcatctt tcagcgacgg ttcagcaaag
    30601 ccctgtttga tcaccagcga ttcagggtta atcaccgaca ggaaatcatc cgcagcacct
    30661 gggttcggca cgctgaacag acggtcatac aaacggattt caaccggcag cgcatgtgcc
    30721 gcgctcaccc agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag
    30781 gtatcggcgt cataagtaca gaagatggtg gtgatattac cttcggcatc tttctcgacg
    30841 cgttctgcct taatcacata agcattacgc agacgcactt ctttacccag caccagacgt
    30901 ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat caatccaaat ctcaccgcta
    30961 aacggcacct gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt
    31021 tcgccttcgc cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg
    31081 cgcggcgcat tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg
    31141 gtgttgtcct gcttggtcac gccaatgcgt ttgcagaact cacgaataga agccgcagtg
    31201 taaccacgac gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg
    31261 tgcttatcgg tcaccaacag gttcagctta cgcttggaca tcacagtgta ttccagattc
    31321 aggcgcgaga attcgtactg gcgcgggtga acaggaatgg tgatgttgtc cagtacccag
    31381 tcgtacagac gacggttatc ctggaactca agcgtacaca gagagtgcgt aataccttcc
    31441 agggcatcgc tgatgcagtg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca
    31501 gtctggtggt gttcagcaaa tttaatgcga tacagcaccg gatcgcgcat cacgataaac
    31561 ggtgaagcca tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accgccggta
    31621 cgcattttct cgaacagcgc caggttctct tcaacgctac ggtcgcggta cgggctgttt
    31681 ttacccggct gtgtcagggt gccacggtat tcgcggatct gttccggcgt cagttcatca
    31741 acgtacgcca ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa
    31801 tcggaggagt aacggacgtt accagaccag tgaaaaccta accactcaac gtcgtttttg
    31861 atcgaatcga catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg
    31921 cactggcctt tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatgaccg
    31981 atatgcagat agccattcgg ctccggcggg aaacgggtgt gtactgtggt gtgcttacca
    32041 ctggccagat cttcatcgat gatctgacga ataaagttag tcgggcgggc ttctgcctca
    32101 ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg
    32161 acaggctgac aactgttagt tagtgaaaat cgaaaatctc actgagatta tggggatgat
    32221 tgatgaaaaa aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct
    32281 caagcgtcgc atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc
    32341 cgccacaaca tggccctgag ctttaatgat caagccactg aaatcatcga tattgctgca
    32401 aaccaccggg ctaatcatcg aacgggcgtt agcgttcagg taatccagat ccatttccag
    32461 aatcggttgt cctgcgctta cctgcgcgcc ctcttccacc agacgtttaa agcctttacc
    32521 ttccagcgct acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc
    32581 caggcagaac gcgtggttgg tgttgaagat tttcacgatt gttcctgcgg ctggtgatac
    32641 gacgatttta tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc
    32701 ttcgtcagga acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc
    32761 gatagatacc gcgtttggta cagcctgcgg ttttgctaca ggagcggcag ttgccggagt
    32821 tgcttcagca gaggcagctg ctaccgggcc acgggcaacg actttcttca tcgcatcgcc
    32881 gatggattct gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gttttactac
    32941 accagaagca cccagacgtt tacacatcgt atcgttaacg cgggcagagt cagccactgt
    33001 aaggcgcaga cgggtgatac aggcgtcaat tgctttcagg ttgtcagtgc cgccaaccgc
    33061 agcaatatag ttggtcgcca gttgattgag gccttcttca gtgttgctgt tggcttcttc
    33121 agtaacgatc tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat
    33181 aaccaaactg aacaccacga agtagatagc gaagaagaca acacccataa ccagcagcat
    33241 ccagacgttc tggctggcgg ccggcaggtt atacatcaac gcgtagtcga tagcccccgc
    33301 agagaaagag aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt
    33361 cagcagtgcg tgcaggaggt acagcagcgg agcaaggaac atgaacagga attccagcgg
    33421 ctcagtcaca ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat
    33481 cggacgacgc tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa
    33541 catcatgatc gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa
    33601 acggttgatg tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa
    33661 ccaggcgatg gtgttcagca cctgatgcag accggttggg atcaacagac ggttgatgaa
    33721 accaaagata ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat
    33781 agcgtgctgt accggcggcc agacataacc aaaaatggcc gccagcacca ggcagaagaa
    33841 gccggtggca atcggcacaa agcgtttgcc gccgaagaaa ctcaggaagt ccggcagttt
    33901 aatatcggac caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag
    33961 tacacccatg tttatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc
    34021 taccgcaccc gccagtgccg ccgcaccagc gctgtctttc gaccagctgg atgccacacc
    34081 gatggcgaag attaatgcga ggttatcaaa aatcgcaccg cccgcctggg caataaacgc
    34141 aacgttaagt aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc
    34201 gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt
    34261 ccccctacga gaaccctatt tggctcgttt aaagccgtat ttttattatg ctgcaaattg
    34321 tactgccgat gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt
    34381 aattcgtatc gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc
    34441 atcactccct tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaggtcg
    34501 gattgacgcc tgtctgcgca aatccaggtt acgcttaaag atgcctaatc cgccaacgac
    34561 ttacatttta cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg
    34621 tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccgactgctg
    34681 atcgtccgtt tgttctgggc ctgccaactg gcggcacgcc aatgaccacc tataaagcgt
    34741 tagtcgaaat gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg
    34801 acgaatatgt cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta
    34861 atttcttcga tcacgttgat attccagcag aaaatatcaa ccttctcaac ggcaacgccc
    34921 cggatatcga cgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc
    34981 atctgtttat gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt
    35041 ctctggcttc tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc
    35101 gtttctttga taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta
    35161 cactgctgga tgctgaagaa gtgatgattc tggtgctggg tagccagaaa gcactggcgc
    35221 tgcaggccgc agttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc
    35281 atccgaaagc catcatggtg tgcgatgagc cttccaccat ggagctgaaa gttaagactt
    35341 taagatattt caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc
    35401 cctggctcct tgctcagggc aatatttttt aaaatcgggg gtcagaatgt atgcattaac
    35461 ccagggccgg atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga
    35521 tggcctgatt aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc
    35581 actgaacggg gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg
    35641 cgtacagttt aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa
    35701 agccaatgag aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga
    35761 gctgatgaaa cagggcgtgc gcgttatgcg cgagtacctg gcaaaacatc cgaatcaggc
    35821 gttaggtctg catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc
    35881 gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc ctgtgtaaga acgccgacgt
    35941 cattaccaaa gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc
    36001 aaatgccggg attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcgaaagc
    36061 cggtttccgc gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac
    36121 cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat
    36181 tatcgctgat ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg
    36241 cgacaaactg tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt
    36301 catttttgcg ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac
    36361 gttaagcggt tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg
    36421 tatcgcactg gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattggcgt
    36481 tgagaaacgt cttggcacac tcgccgcagg taaagtagcc aacctgaccg cattcacacc
    36541 tgattttaaa atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa
    36601 agtatgacac caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac
    36661 agcgcggcgg tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc
    36721 gagcaaagcc agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc
    36781 gggctgatca aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc
    36841 gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc
    36901 attactctgt ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa
    36961 cgtacccagc agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc
    37021 taccagcgca agctgcgcga gctgatcgcc atttccgtta tcctgccagg acttgttgac
    37081 ccggacagcg gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta
    37141 gaagcactgg aagagcgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg
    37201 gcgctggcgg agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt
    37261 gtccatcgcg gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac
    37321 ggtaacgtcg gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc
    37381 ggcaactttg gctgcctgga aactatcgct gccaacgccg ctattgaaca acgggtgttg
    37441 aatctgttaa agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact
    37501 atctgcaaag ccgcaaacaa aggcgacagc ctggcctcgg aagtgattga gtatgttggt
    37561 cgtcatctgg gcaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt
    37621 attgccggag aaatcacgga agccgataaa gtgctgctcc ctgctattga aagctgcatt
    37681 aatactcagg cgctgaaggc gtttcgcact aatctgccgg tggtacgttc tgagctggat
    37741 caccgctcgg caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg
    37801 ctccagcatt tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt
    37861 ctgggtagtc catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg
    37921 ataacgtcgc cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc
    37981 cgctggtgtt gctgaccaac tatccttcgc agaccgggca agatctggcg aaccgctttg
    38041 ccactgccgg tgtcgatgta cctgacagcg tgttttatac ctctgcgatg gcgaccgccg
    38101 atttcctgcg tcgccaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc
    38161 atgaactgta caaagccggt ttcactatta ccgatgtgaa ccctgatttt gtgattgttg
    38221 gcgaaacgcg ttcctacaac tgggacatga tgcataaagc ggcctatttc gtcgccaacg
    38281 gcgcacgctt tatcgccact aatccggata ctcacggacg cggtttttat ccagcctgtg
    38341 gcgcactgtg cgcaggcatt gagaaaatct ccgggcgcaa accgttctat gttggtaaac
    38401 ccagcccgtg gatcatccgc gcggcattaa acaaaatgca ggcgcactcg gaagaaacgg
    38461 tgattgtcgg cgataacctg cgcaccgata ttctggcagg cttccaggcg ggtctggaga
    38521 cgattctggt gctctctggt gtttcatcgc ttgacgatat cgacagcatg ccattccgcc
    38581 ccagctggat ttacccgtcg gtcgctgaaa tcgacgttat ctgaatagca accacgtctg
    38641 cggacgtggt tggttctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc
    38701 ctacgccgcc cctgcaatat atttaatttt catgcttttg taggccggat aaggcgttca
    38761 cgccgcatcc ggcatgaaca aatcgcactt tgtcagcaat tcggcccccg taaaatataa
    38821 ccattcaata aaagccgcta aaaattatcg aatcatcaat aaacacgccg ctacaaccca
    38881 cgttttttca tcaatcagca atcgccctta cgttttttat ttttccactg ccaaatagct
    38941 tgcgcacagt gctgctttgc ggcatttttt taaacaagca aacacaacaa gcagcaaaca
    39001 ccaggttaac ggagaaggtt atgtgttcaa tttttggcgt attcgatatt aaaacagacg
    39061 cagttgaact gcgtaaaaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg
    39121 actggtccgg tatttatgcc agcgataaag ccattctcgc tcacgaacgt ctgtcaattg
    39181 ttgacgttaa cgcgggggcg caacctctct acaaccaaca aaaaacccac gtactggcgg
    39241 taaacggtga aatctacaat caccaggcat tgcgcgctga atatggcgat cgttatcagt
    39301 tccagacagg gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat
    39361 ttctcgacga cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct
    39421 acctgattgg tcgcgaccat ctggggatca tcccactgta tatgggctat gacgaacacg
    39481 gtcagctgta tgtagcctca gaaatgaaag ccctggtgcc agtttgccgc acgattaaag
    39541 agttcccggc ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc
    39601 gcgactggtt cgactttgat gcagtgaaag ataatgtcac cgacaaaaac gaactgcgtc
    39661 aggcactgga agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctgc
    39721 tttctggtgg tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc
    39781 gcgtggaaga tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag
    39841 gtctgccggg ttcaccggat ctgaaagcag cccaggaagt ggcaaaccat ctgggtactg
    39901 tgcatcatga aattcacttc actgtacagg aaggtctgga cgccatccgc gacgtgattt
    39961 accacatcga aacctatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt
    40021 cgcgtaagat caaagcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag
    40081 tgttcggcgg ttatctttac ttccacaaag caccgaatgc caaagaactg catgaagaga
    40141 cggtgcgtaa actgctggcc ctgcatatgt atgactgcgc gcgtgccaac aaagcgatgt
    40201 cagcctgggg cgtggaagca cgcgttccgt tcctcgacaa gaaattcctc gacgtggcga
    40261 tgcgcattaa cccgcaggat aaaatgtgcg gtaacggcaa gatggaaaaa cacatcctgc
    40321 gtgaatgttt tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct
    40381 ccgatggcgt cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt
    40441 ctgatcaaca gctggaaact gcccgcttcc gcttcccgta caacacaccg acctcaaaag
    40501 aagcgtatct gtaccgggag atctttgaag aactgttccc gcttccgagc gccgctgagt
    40561 gcgtgccggg cggtccttcc gtcgcttgtt cttccgctaa agcgatcgaa tgggatgaag
    40621 cgttcaaaaa aatggacgat ccgtctggtc gtgcggttgg tgtccaccag tcggcgtata
    40681 agtaagaaat gcgaaacagc cccggtgaaa tccggggctg tttgacaact catcactgta
    40741 ggccagtatt tcatagatta atttcccacc actattgtta aattccgaat ttcatcctgt
    40801 atgacaaaag aatggctgta gggtgggaaa aaacacaaag taaacaataa ttgacgaata
    40861 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    40921 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    40981 gcgaaaaaaa agatggctac gtagctcagt tggttagagc acatcactca taatgatggg
    41041 gtcacaggtt cgaatcccgt cgtagccacc atcttttttg cgggagtggc gaaattggta
    41101 gacgcaccag atttaggttc tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc
    41161 accattcacc agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg caccggtttt
    41221 tgataccggc attccctggt tcgaatccag gtaccccagc catcttcttc gagtaagcgg
    41281 ttcaccgccc ggttattggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    41341 ttccctggtt cgaatccagg taccccagcc atcgaagaaa caatctggct acgtagctca
    41401 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    41461 ccaaattctg aatgtgtcga atatgttcgg caaattcaaa accaatttgt tggggtatcg
    41521 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    41581 agccaattta ttcaagacgc ttaccttgta agtgcaccca gttggggtat cgccaagcgg
    41641 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    41701 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    41761 tgttgatacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    41821 ggttggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    41881 acgtttcaac acgccagcac gctccgccgc catcaaacca agattacgca caaaacgcag
    41941 cggcggcaga ttattactga atccggcata aaacagatcc ataccgctct gcataatgaa
    42001 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    42061 gccgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    42121 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaac
    42181 ccctggctgc acatactgca acgcatggcg acgcgtcagc ggaaacgcac cagcggcaag
    42241 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagctgtgc
    42301 catattcata ttctgcaact ggcgaatacg cgccggagag tcataccaca ccagcgatgc
    42361 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggagtaaatt gctgccaggt
    42421 gctatcgccg ggatcgttct cgcactggac gctaatcaac atgcacgact gcgcatactg
    42481 ccatgcatga acgccaattc ccgccatctg ccgcacctgc gaatttgcgc cgtcggcacc
    42541 aatcaccagc ttcgcgcgaa tcacttcacc gcctttcagc tccagctcct gaagatcatt
    42601 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    42661 cagcgcctgc cacaatgcct gttgcaggac agtgttttct accatatagc caagcaacgg
    42721 caactttaat tcggctgcgt caaataccac atgcgccgtt tcccactccc acgtttccag
    42781 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    42841 caatgatacc gatgcggcgc tgatcgccga gatccgcacg tccggttggc tatcagcgac
    42901 aaacggtgca ggttctgcgt gctcgatcac cgttaccgta aatccgtgct gtgccagccc
    42961 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    43021 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    43081 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    43141 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    43201 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgccaccca
    43261 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctgctgctga acacctgctc
    43321 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    43381 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    43441 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    43501 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    43561 cccggaaatc gagaagtttg accgtctacc ggaaccgcgc gccgaagggc cgaccgcgtt
    43621 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    43681 tggtgaagag gtaagccgtc cgtccgacga tattctgttt gagattgccc agcttgcagc
    43741 tcagggcgtg cgtgaagtca acctgctcgg tcaaaacgtg aacgcctggc gtggtgaaaa
    43801 ctacgacggc accactggat cgtttgccga tctgctgcgt ctggttgctg cgatcgacgg
    43861 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    43921 agtgtaccgc gacacgccgg agctggtgag cttcctgcat ctgccggtac agagcggttc
    43981 cgatcgcatt ctgaacctga tgggccgtac ccatacggcg ctggagtaca aagcgatcat
    44041 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca tcgttggctt
    44101 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    44161 cgacatgagc tacagcttta tcttctctgc acgtccggga acgcctgccg ctgatatggt
    44221 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    44281 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    44341 agggacttca cgtaagagca tcatggagct ttccgggcgt accgaaaata atcgtgtggt
    44401 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    44461 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcgt
    44521 ggcagaaaca ccggaatcgg tgattgcccg tactcgcaaa gaaaacgacc ttggcgtggg
    44581 ttattatcag ccgtaattct caggcccgcc gttccggtgg gccttgtttt ttctctcttt
    44641 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    44701 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    44761 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    44821 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    44881 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    44941 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    45001 atccaccttg cgattaaaga agcacgggta ctggagcaaa gcgcggagag cgtgccggag
    45061 tacggcaaag cggtcaatat caaaaccaaa cgcggcgtaa ttaagccgcg cacgccaaac
    45121 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    45181 ggtacgggta aaacctacct ggcagtggct gcagcagttg atgccctgga gcgtcaggaa
    45241 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    45301 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    45361 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    45421 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcct ttatcattct cgatgagagc
    45481 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    45541 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    45601 cgtcatgcca tcgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    45661 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcctatga agcctgggaa
    45721 gaagccgaac aaaaacgtaa agcggcgctg gcggcagaac gcaagcgcga agaacaggaa
    45781 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataac tccggattgc
    45841 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    45901 cggaagtgac gattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    45961 gcggtaaaga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cccggcatgg
    46021 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagcacagg
    46081 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    46141 tgttaggtta cgatcacatc gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    46201 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    46261 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    46321 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    46381 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    46441 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    46501 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    46561 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    46621 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    46681 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    46741 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    46801 aatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    46861 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    46921 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    46981 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    47041 tgaagaagtc gacactatcg gtggtctggt gatgcaggca tttgggcatc ttccggcacg
    47101 tggcgaaacc atcgacatcg acggttacca gttcaaagtg gcgatggccg acagtcggcg
    47161 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccgaagc tggatgaata
    47221 aaaccgaaac tggatagata actacatggc ttttgcctca ttaattgaac gccagcgcat
    47281 tcgcctgctg ctggcgttat tatgcggtgc ctgcggaacg ctggccttct ctccttacga
    47341 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgccg
    47401 tccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    47461 taactgggtc tatgtcagca tcgcgacctt tggcggaatg cctggcccgg ttaacatctt
    47521 cctggtggta ctgctggcag cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    47581 gtcgcgcctg tggccgaaaa ccacctggct gcgcgtggcg attgccgccc ctgctctctg
    47641 gcaagtgacc gagtttctgc gcggttgggt actgacaggc ttcccatggt tacagttcgg
    47701 ctatagccag attgacggcc cgttaaaagg gctggcaccg ataatgggcg tggaagccat
    47761 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    47821 gcgtccgctg gtagtggccg tcgtgctgtt tgccctaccc ttcccgctac gttacatcca
    47881 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    47941 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctgaagattt actacaacgc
    48001 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    48061 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    48121 tagctcgctg gtcaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    48181 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    48241 ttataacaaa aaccatctgg tgccgtttgg cgaatttgtc ccgctggagt cgattctgcg
    48301 tccgttagcg ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    48361 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agatcattct
    48421 cggcgagcaa gtgcgcgata acttccgccc ggataccgac tatctgctga ctatctccaa
    48481 cgatgcgtgg tttggtaagt ctattggtcc atggcagcac ttccagatgg cgcgaatgcg
    48541 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    48601 tggcccgcag ggtgagattc aggcgatgat cccgcagttc acccgcgagg tgttaaccac
    48661 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    48721 ggtgctgacg gcactgtttg gttttgccgc tgtgctgatg agtctgcgtc agcgacgtaa
    48781 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    48841 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    48901 tgttatacaa ggcaaagctt taaccagcat cagtacaacc cggttgcacc acagcaatcc
    48961 accggtagca ccaaaagagt gcattttaag aattttgctt cggaatggtg cggtgcacct
    49021 cgcaaatata aacaataaaa cagcaatatc tttacattta gccaaactaa atgttaccta
    49081 acaggcacaa cactgcacaa taaagttgca gatgataaca acacaaacac tcacaacggg
    49141 tatccatgcg ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc
    49201 cacagcaatc ctggcccttg cgctttccgc aggactggca caggcagatg acgccgcccc
    49261 ggcagcgggc agtacgctgg acaaaatcgc caaaaacggc gtgattgtcg tcggtcaccg
    49321 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttattcgca
    49381 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    49441 ggtgaaactg attccgatca cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt
    49501 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    49561 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    49621 ttttgccgac ctgaaaggca aagctgtagt cgtcacttcc ggcactacct ctgaagtttt
    49681 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    49741 cggtgactct ttccgtaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc
    49801 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    49861 gccgcagtct caggaagcct atggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    49921 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    49981 taaatggttc aaaaatccca ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    50041 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    50101 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    50161 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    50221 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    50281 gtattttttt acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg
    50341 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    50401 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    50461 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    50521 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    50581 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    50641 aacaggtacg cgccgcgatt cagtcgctgc cgcgcgggca aaaaaatgcc gcgctggcga
    50701 tgggtctgac gctaccacag gcttaccgct acgtactgct gcctaatgct tatcgcgtta
    50761 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    50821 ctatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgattac tcggcccacg
    50881 cctgggagtc gtttaccgcc attacgcttg cgtatgtgtt gattaacgct tttatcatgc
    50941 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    51001 gtacgagttt gactggagtt ccattgtccc ttccctgcca tatctgctcg acgggctggt
    51061 gatcacgctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct
    51121 ggcggtgatg cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa
    51181 cgtattccgc tcgattcctt tagttatggt tttgctgtgg ttttacctga ttgtgccggg
    51241 ttttctgcaa aacgtgctgg gattatcacc aaaaaatgat atccgtttaa tttcggcgat
    51301 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca
    51361 aagtatttct cgtggtcagt cgagcgccgc gctggcgttg ggaatgactc actggcagtc
    51421 gatgaaactg attattctgc cgcaggcgtt ccgcgcgatg gtgccgctgc tgctcactca
    51481 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    51541 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc
    51601 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    51661 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    51721 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    51781 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    51841 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgt
    51901 tctcgcgtcg ggatggtatt ccaacatttc gagctgttcc ctcatctgtc gattatcgaa
    51961 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgccggc tcgtgaaaaa
    52021 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    52081 ctttccggcg gccagcagca gcgtgtggca attgctcgcg cattgtgtat ggatcctatt
    52141 gcgatgctgt tcgacgaacc cacatcggca ctagacccgg agatgatcaa cgaagtactg
    52201 gacgtgatgg tggaactggc gaacgagggg atgaccatga tggtggtgac ccacgaaatg
    52261 ggctttgccc gtaaagtggc gaatcgggtg atctttatgg atgagggtaa aattgttgaa
    52321 gactcgccga aagatgcgtt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    52381 gcgaaaatcc tgcattaatc actctggcgc gcatacccaa ttgcgcgcca tctcacgctt
    52441 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    52501 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgacgac
    52561 gctatcgcaa tagttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct
    52621 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    52681 cttaatcgca ccgatattcc ggtagcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    52741 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    52801 gcgttcgcac cgcaaaactg tacggcggta gagctaatgg cgaaaacgct gcgtgaaagt
    52861 gcggaacctg tcaccattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    52921 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    52981 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    53041 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcacaaagcg
    53101 caaatccacg ttgaggacac cgagcgtttc cgcgcgattg gtaacccggt ttcaaccatt
    53161 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    53221 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    53281 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg gatgacggtt
    53341 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat
    53401 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acacacgtca
    53461 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tgtttatctc tttttctttc
    53521 aagatataaa tgcaatggat aatgcagaac tcgccattgg tatcgatctc ggtactacca
    53581 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    53641 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    53701 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    53761 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    53821 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tatgcaacgt ccgattaaag
    53881 atgtggtgat ctctgttccg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    53941 cagcggagtt agccggttta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    54001 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    54061 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    54121 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gctggtcgat gaggttttaa
    54181 aacgcgcgga tgtcgccagg accacgctta acgagagtga actggcagcc ttgtatgcct
    54241 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg cagtaccagg
    54301 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    54361 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcacgtctg aagccgagtc
    54421 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    54481 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    54541 tgggcgcagc aatccaggct gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    54601 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgtcagggc gtttccggca
    54661 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    54721 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    54781 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    54841 atcagtcgat tgatattcgc tttagttatg atatcaacgg tttgcttgaa gttgacgtgc
    54901 ttctcgaaga cggcagcgtt aagtccagag tgattaatca cagtcctgta acattgagcg
    54961 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    55021 tgctcatcaa ccgtaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    55081 acgagcgaga agagattggc caggtgatca ccgattttga tgcggcgttg cagtcaaacg
    55141 atatggcccg cgtcgatgag gtcaggcggc gggcgagtga ttatttagcc attgagatcc
    55201 cataaatacg gctgccggat gctgcgtaaa cgctgcatcc ggcatcggcg caaaaaacac
    55261 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctatcacc agcaccgtta
    55321 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    55381 catgattttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt ttcactttcg
    55441 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccacct tccagccttg
    55501 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    55561 cccgatacaa atccagtaac ggtgaatctt gctctgtctc gctaccgact ctcgatttcc
    55621 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    55681 atagttttcc gctatgtaaa atttccgccc ataacagacg cgtccgggcg ctctcaccag
    55741 gaacatattt caacggttta ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    55801 ctaataacgc gaaataatcc gggcaatgtt gtgcgctcca ctgcaacaac aaattatcgg
    55861 cttgtgtaga tgggtaactt ccccagtacg cgcagagatc ggcaagcagg ctttccccct
    55921 cgccgcagta caaacgttgt gcacacaagt aatatcgggc atcttcgctt tctggttctt
    55981 ccatttgcca ctgttgagcg acaggaatga gttccgccat acctgtttgc accgagctgt
    56041 accagcgcaa cagtttgcga tgcaacttcg catcatcagg aatgaaccac ggaccatcca
    56101 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcatcgttcc agcgcgaagt
    56161 aatactcaac cgtctggttc tgcaacgcaa cgggatattc cagaaggcta aggaaatcga
    56221 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    56281 cgctgtcatt aaacgaatgc cagttcaaac gctctgccag caaagaagca caattcaatg
    56341 aaagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    56401 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    56461 cactttctga atccagtaat gccctgaatg cacgtagtat ttcatgttca gcggcggcat
    56521 ctttatcttc attatctgct tcctcgacag gatttaccgc cagtcgtagc gcctcttcat
    56581 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    56641 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    56701 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    56761 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    56821 gaacatacga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagt
    56881 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    56941 gtgcatccag ctgtgatagt aatggtgagc tcgtacatag acgcgcgatt gcagccataa
    57001 actctcgtct tccgtcagcc acttgttgag cacaccgacc cgtgacagaa accccattcg
    57061 aacataatcc caagctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    57121 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    57181 ggaagagcga tgccatgtca gatataagcc agctaaatca ttagcatgac catcatcagt
    57241 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    57301 atcgaattct tttatcccag tatacatttt tggcgagtca tgcgcgggtt tgcaaccctg
    57361 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttatt
//
LOCUS       DAPGRN010000016        53590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPGRN010000016 DAPGRN010000000
VERSION     DAPGRN010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.016"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..137
                     /locus_tag="RHW33_001094"
     CDS             <1..137
                     /locus_tag="RHW33_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HDV1644619.1"
                     /translation="KGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM
                     "
     gene            428..1156
                     /gene="mpaA"
                     /locus_tag="RHW33_001095"
     CDS             428..1156
                     /gene="mpaA"
                     /locus_tag="RHW33_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV1644620.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1131..2096)
                     /gene="ycjG"
                     /locus_tag="RHW33_001096"
     CDS             complement(1131..2096)
                     /gene="ycjG"
                     /locus_tag="RHW33_001096"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV1644621.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDAMLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2215..2721
                     /gene="tpx"
                     /locus_tag="RHW33_001097"
     CDS             2215..2721
                     /gene="tpx"
                     /locus_tag="RHW33_001097"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV1644622.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2765..4306)
                     /gene="tyrR"
                     /locus_tag="RHW33_001098"
     CDS             complement(2765..4306)
                     /gene="tyrR"
                     /locus_tag="RHW33_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV1644623.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4454..5515)
                     /locus_tag="RHW33_001099"
     CDS             complement(4454..5515)
                     /locus_tag="RHW33_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV1644624.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5512..6909)
                     /locus_tag="RHW33_001100"
     CDS             complement(5512..6909)
                     /locus_tag="RHW33_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV1644625.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRYKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQAMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7064..8062
                     /locus_tag="RHW33_001101"
     CDS             7064..8062
                     /locus_tag="RHW33_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1644626.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIENGHKNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8173..9078)
                     /gene="ompG"
                     /locus_tag="RHW33_001102"
     CDS             complement(8173..9078)
                     /gene="ompG"
                     /locus_tag="RHW33_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV1644627.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(9123..10205)
                     /locus_tag="RHW33_001103"
     CDS             complement(9123..10205)
                     /locus_tag="RHW33_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1644628.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(10219..10878)
                     /gene="pgmB"
                     /locus_tag="RHW33_001104"
     CDS             complement(10219..10878)
                     /gene="pgmB"
                     /locus_tag="RHW33_001104"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV1644629.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERTQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGARLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            complement(10875..13142)
                     /gene="ycjT"
                     /locus_tag="RHW33_001105"
     CDS             complement(10875..13142)
                     /gene="ycjT"
                     /locus_tag="RHW33_001105"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV1644630.1"
                     /translation="MTRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIVINGEVFSLSREAWQRELDFASGELR
                     RSVVWCTSNDARYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQTALDEWGGASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQATGDESFIAHEGMALLLETAK
                     FWISRAVRVNNRLEIHDVIGPDEYTEHVNNNAYTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLSEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(13139..14194)
                     /locus_tag="RHW33_001106"
     CDS             complement(13139..14194)
                     /locus_tag="RHW33_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1644631.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVNPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQARVMRDTARRQGKVLAYDFHHRFALDTQQLREQVTNGILGEIYVTTARA
                     LRRCGVPGWGVYTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRETADDNRHLRSMEAFINHVQGKPMMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(14204..14992)
                     /locus_tag="RHW33_001107"
     CDS             complement(14204..14992)
                     /locus_tag="RHW33_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV1644632.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSQRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(15010..16062)
                     /locus_tag="RHW33_001108"
     CDS             complement(15010..16062)
                     /locus_tag="RHW33_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV1644633.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSAVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(16093..16935)
                     /locus_tag="RHW33_001109"
     CDS             complement(16093..16935)
                     /locus_tag="RHW33_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV1644634.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(16922..17803)
                     /locus_tag="RHW33_001110"
     CDS             complement(16922..17803)
                     /locus_tag="RHW33_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV1644635.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGALVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(17824..19116)
                     /locus_tag="RHW33_001111"
     CDS             complement(17824..19116)
                     /locus_tag="RHW33_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1644636.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQV
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDSPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLSTTLQASQKKLDELIEQH"
     gene            complement(19131..20810)
                     /gene="ycjM"
                     /locus_tag="RHW33_001112"
     CDS             complement(19131..20810)
                     /gene="ycjM"
                     /locus_tag="RHW33_001112"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV1644637.1"
                     /translation="MKQKITDYLDKIYGGTFTSTHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYLQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSSVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPDVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDIAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSGKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     YREEITRELNDEATLRHAVYHELSRLIILRRSHNEFHPDNNFTIDTVNSSVMRIQRSN
                     AEGNCLTGLFNVSKNIQHVNITGLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(21021..21335)
                     /gene="pspE"
                     /locus_tag="RHW33_001113"
     CDS             complement(21021..21335)
                     /gene="pspE"
                     /locus_tag="RHW33_001113"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV1644638.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(21410..21631)
                     /gene="pspD"
                     /locus_tag="RHW33_001114"
     CDS             complement(21410..21631)
                     /gene="pspD"
                     /locus_tag="RHW33_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV1644639.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(21640..21999)
                     /gene="pspC"
                     /locus_tag="RHW33_001115"
     CDS             complement(21640..21999)
                     /gene="pspC"
                     /locus_tag="RHW33_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV1644640.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(21999..22223)
                     /gene="pspB"
                     /locus_tag="RHW33_001116"
     CDS             complement(21999..22223)
                     /gene="pspB"
                     /locus_tag="RHW33_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV1644641.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(22277..22945)
                     /gene="pspA"
                     /locus_tag="RHW33_001117"
     CDS             complement(22277..22945)
                     /gene="pspA"
                     /locus_tag="RHW33_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV1644642.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            23112..24089
                     /gene="pspF"
                     /locus_tag="RHW33_001118"
     CDS             23112..24089
                     /gene="pspF"
                     /locus_tag="RHW33_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV1644643.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(24209..25474)
                     /gene="puuE"
                     /locus_tag="RHW33_001119"
     CDS             complement(24209..25474)
                     /gene="puuE"
                     /locus_tag="RHW33_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV1644644.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVAYPSDLHGVSTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(25512..26792)
                     /gene="puuB"
                     /locus_tag="RHW33_001120"
     CDS             complement(25512..26792)
                     /gene="puuB"
                     /locus_tag="RHW33_001120"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV1644645.1"
                     /translation="MTEHTSSYYAASANKYTPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLEMLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTTKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(26794..28281)
                     /gene="puuC"
                     /locus_tag="RHW33_001121"
     CDS             complement(26794..28281)
                     /gene="puuC"
                     /locus_tag="RHW33_001121"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV1644646.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEANAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLDLLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFKSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(28431..28988)
                     /gene="puuR"
                     /locus_tag="RHW33_001122"
     CDS             complement(28431..28988)
                     /gene="puuR"
                     /locus_tag="RHW33_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV1644647.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(29015..29779)
                     /gene="puuD"
                     /locus_tag="RHW33_001123"
     CDS             complement(29015..29779)
                     /gene="puuD"
                     /locus_tag="RHW33_001123"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV1644648.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            29991..31409
                     /gene="puuA"
                     /locus_tag="RHW33_001124"
     CDS             29991..31409
                     /gene="puuA"
                     /locus_tag="RHW33_001124"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV1644649.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     VGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            31558..31722
                     /locus_tag="RHW33_001125"
     CDS             31558..31722
                     /locus_tag="RHW33_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644650.1"
                     /translation="MPMIKSPHGAGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            31712..33097
                     /gene="puuP"
                     /locus_tag="RHW33_001126"
     CDS             31712..33097
                     /gene="puuP"
                     /locus_tag="RHW33_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV1644651.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMLVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVRET"
     gene            33232..33477
                     /locus_tag="RHW33_001127"
     CDS             33232..33477
                     /locus_tag="RHW33_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV1644652.1"
                     /translation="MNHDIPLKYFDIADEYATECADPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            33790..35433
                     /gene="sapA"
                     /locus_tag="RHW33_001128"
     CDS             33790..35433
                     /gene="sapA"
                     /locus_tag="RHW33_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV1644653.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPEPTPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            35430..36395
                     /gene="sapB"
                     /locus_tag="RHW33_001129"
     CDS             35430..36395
                     /gene="sapB"
                     /locus_tag="RHW33_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV1644654.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            36382..37272
                     /gene="sapC"
                     /locus_tag="RHW33_001130"
     CDS             36382..37272
                     /gene="sapC"
                     /locus_tag="RHW33_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV1644655.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            37272..38264
                     /gene="sapD"
                     /locus_tag="RHW33_001131"
     CDS             37272..38264
                     /gene="sapD"
                     /locus_tag="RHW33_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV1644656.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            38266..39072
                     /gene="sapF"
                     /locus_tag="RHW33_001132"
     CDS             38266..39072
                     /gene="sapF"
                     /locus_tag="RHW33_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV1644657.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTNLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(39122..40285)
                     /locus_tag="RHW33_001133"
     CDS             complement(39122..40285)
                     /locus_tag="RHW33_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HDV1644658.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCVLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWTQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGFGGETLLKMSLAITVCYTLAFLVALTRIDNQK"
     gene            complement(40294..41667)
                     /locus_tag="RHW33_001134"
     CDS             complement(40294..41667)
                     /locus_tag="RHW33_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV1644659.1"
                     /translation="MLRRTLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSFTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMTSAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLEENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFTPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQTGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(41671..44778)
                     /locus_tag="RHW33_001135"
     CDS             complement(41671..44778)
                     /locus_tag="RHW33_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV1644660.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPNTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRAARTMGLYLVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     SRRQE"
     gene            complement(44778..45899)
                     /locus_tag="RHW33_001136"
     CDS             complement(44778..45899)
                     /locus_tag="RHW33_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDV1644661.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTVKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLIRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMEDAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            complement(46048..46614)
                     /locus_tag="RHW33_001137"
     CDS             complement(46048..46614)
                     /locus_tag="RHW33_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV1644662.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            47092..47880
                     /gene="fabI"
                     /locus_tag="RHW33_001138"
     CDS             47092..47880
                     /gene="fabI"
                     /locus_tag="RHW33_001138"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV1644663.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSNIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            48024..49151
                     /locus_tag="RHW33_001139"
     CDS             48024..49151
                     /locus_tag="RHW33_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV1644664.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LEKETLAPFLSWLDPARVLAVDLFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSTRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLAEILSLHPISLSLLERLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFAACMDSSNEYKKISTLLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            49219..51153
                     /gene="rnb"
                     /locus_tag="RHW33_001140"
     CDS             49219..51153
                     /gene="rnb"
                     /locus_tag="RHW33_001140"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV1644665.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFSA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(51231..51335)
                     /locus_tag="RHW33_001141"
     CDS             complement(51231..51335)
                     /locus_tag="RHW33_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644666.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            51389..53374
                     /gene="pdeR"
                     /locus_tag="RHW33_001142"
     CDS             51389..53374
                     /gene="pdeR"
                     /locus_tag="RHW33_001142"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV1644667.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPERTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLNEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT        12746 a        14373 c        13331 g        13140 t
ORIGIN      
        1 tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt aattacagat atcatcgata
       61 aacctgagaa acacattggt gagaatatag gcatcaatca acccgggact gatggcgata
      121 agccattctt catgtaaatc aattatatag ctaccatcgt actgtactat gtgcagtacg
      181 atttattaaa cttattaagg tgaactgatc aatcagatgc gattaacatg tgtagaacca
      241 gtaagaataa aatacgccat gtatataaca ttacgacaat tctaatatct gcacatcaat
      301 gaaaatgcaa acaacttccg gcaaaaaagt gatacaagct cgatgacata gcgttgaaga
      361 aaaggatttg ataatccgca aaatttgcac aacactgcct gtaatacttc ctgaaccaga
      421 gacgatcatg accgtaaccc gcccacgcgc cgaacgcggc gcatttccgc ccggaacaga
      481 acattacgga cgttcattat tgggcgcgcc gttaatctgg tttccggccc ctgcagccag
      541 tcgcgaaagt ggtttgattc tggctggcac tcacggtgat gaaaactctt cggtcgtcac
      601 gctctcctgt gctctgcgga ctttgacacc ttctttgcgc cgccatcatg tggtgctgtg
      661 tgtgaatcct gacggctgcc agctggggtt acgggccaat gctaatggtg tggatttaaa
      721 cagaaacttt ccggcggcga actggaagga aggtgaaacg gtttatcgct ggaacagcgc
      781 cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa cctggctccg aacctgaaac
      841 ccaggcgctg tgccagctta tacaccgcat tcaacctgcc tgggtggtct cattccacga
      901 tccactggcc tgtattgaag atcccagaca cagcgaatta ggcgaatggc tggctcaggc
      961 gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg cctggctctt tcggcagttg
     1021 gtgtgcagat ctcaacttgc attgcatcac cgcagagttt ccgcccatct cctccgacga
     1081 agccagcgaa aaatatctct ttgcgatggc aaacttgctg cgctggcatc ctaaagatgc
     1141 aattcgcccg tcgtgaactg aagcgccggt tccacatcta ccgccagcca ggtcggtccg
     1201 tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag cggcgctaat ggcccgagag
     1261 gtacacaaca tgcagcccag catcagacga aaaccttgtg cacgcgcttc agtcgccagc
     1321 gccagcgctt ccgtcagacc cccggtttta tcgagcttaa tgttaaccat ctcatagcgc
     1381 cctttcagcg ccttcagatt gctacgagta tgacaacttt catcagcaca aatcggtaac
     1441 ggatgaataa aattctccag tgccgcatcg tcctgcgcag gaagcggttg ttcaagcata
     1501 gcaacgccta aatccgccaa tagctggcaa cgtgccgcca acccttctgc acgccaggat
     1561 tcatttgcat caacgatcag catggcatcg ggcacagctg tgcgaattgc caccatccgc
     1621 tcactgataa gatggttatc cagcttcact ttcaataatt tcgcgccggc ctgccagagt
     1681 gttgatgcgc tattggccat ctgatcaggc gtaccgatga ctaccgtctg tgcagtggtg
     1741 actgtcccgg gaagcgttat gccgatcaaa tcagccagcg attgctgctg ttttcgcgcc
     1801 gccagatccc acaatgcaca atccagcgca ttacgtgctg cgccggcagg gagaattttt
     1861 tgcaactcct cccgtgtcag ccctttctct aattgcggta cgacgctcat aatttgcgcc
     1921 attaccgagg catcactttc tccataacgc ggatacggcg tgcattcgcc ggtgccttta
     1981 ataccctctt cttccagttc aaccaccacc acgcgcgctt cactgcgact tccccgggca
     2041 atcacaaacg gggtatgtaa gggccaggct tcctcaaata ccttaacggt tctcatcttt
     2101 actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat agcgtttgct gtgttattga
     2161 cagttagcat aaactagatg tgacgttaac tatatgtaaa caggaaagat aatcatgtca
     2221 caaaccgttc atttccaggg caacccggtt acagtcgcca actccatccc gcaggcgggt
     2281 agcaaagcgc agacttttac tctcgtggca aaagatctgt ctgacgtcac cctcggtcag
     2341 tttgcgggta aacgcaaagt gctgaacatt ttcccgagta ttgataccgg tgtttgcgcc
     2401 gcatcagtac gtaagtttaa ccaactggca accgagatcg acaacaccgt tgtgctgtgt
     2461 atctctgccg atctgccgtt cgcccagtct cgtttctgcg gcgcagaagg tctgaacaac
     2521 gttatcaccc tctccacttt ccgtaacgct gaatttctgc aagcctacgg tgtggcaatt
     2581 gctgatggcc cactgaaagg tctggcagcg cgtgccgttg tggttattga tgaaaatgac
     2641 aatgtgattt tcagccagct ggtggatgaa atcaccaccg agccggatta cgaagcagct
     2701 ctggctgtac tgaaagcata agttaatatg cctgatggtg ttgcaccatc aggcatattc
     2761 gcgcttactc ttcgttcttc ttctggctta agccatattc ccgcaactta ttggcaatcg
     2821 cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt gctgggataa ttgcgataaa
     2881 gctgggttaa taccgagcgt tcaaaacggc tggtgatttc gtccagcgaa ccttccatcg
     2941 catcttcgcc cacggctacc gttgcggcgt cataatccgg caacaaaata tcctgtggac
     3001 gcagctcata accgtccagt tgtgtcagtg cgcgatagat agcgttcttt aactgccgca
     3061 catttcccgg ccacgcataa cgcgtaagta cagtattcag gtcagcggcc agtttcggac
     3121 gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag ctcagttaac ggcatgatgt
     3181 cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa cacgttcaga cgataataga
     3241 gatcttcacg gaacatgcct ttttgcacca gttcgaccag attcttctgc gtagcgcaaa
     3301 tcacccgcac atcgacatgc acctcatggt cttcaccaac ccgacggaaa gtaccatcat
     3361 taaggaaacg caataatttc gcctgcatcc gcggtgacat ctcacctatt tcatccaaca
     3421 gcaccgaacc accgttcgcc tgctcaaaga atcctttctt cccttccgga gcatgaccaa
     3481 acagttcact ctcgaccgca tcttccggta tagacgcaca gttcagcgcc aggtaaggtt
     3541 tgcccgctct ggggcttgcc tgatggcagg cgtaggcaaa gagatcttta cctgtacctg
     3601 tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag tttctgcgcc tgttcgacaa
     3661 catgcttcat tttcgggctg acagcgacaa tttgactgaa ggcgctgacg tcctgggcgg
     3721 cgacattttg caactgacgg cccatacgaa tcgttgatcg caacatcacc accgcacctg
     3781 tcaggacgtg ttgatcattt tcatcctgaa gataaacagg cgtaatctcc atcaggaagt
     3841 tctgtccatt aataactaca tgctcgttat gggaatcttg cggttcgctt tccagccaac
     3901 gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg gttgcgcagg cgatccaatt
     3961 tttgcccaaa aagctgacag cttgccgggt tcgccatatc cactttgctt ttcatatcga
     4021 cagagagcac aggttcaggc aacgcctcca gtaacgcgct caacgccaga tgctcacgtt
     4081 cggaaggcat ccacgggaca gtacgcacat cggtaacacc tgcaatacgg cgtatttcgg
     4141 ccatcagact gctgaaactc tcaaactcca gttcagcaaa attgaggtag attcgcccaa
     4201 tgggatcaat ctcaataccg cgtaaatcaa tgcctcttag cacgagtaga tcgagtaatt
     4261 cgcgggtcag accgagtcgg tcttcacaaa agacttccag acgcatggga accttcacct
     4321 gaaaaaagaa caattaatat gatgatatga cactatttgg atagcaggaa ggtttctgtc
     4381 aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg cgtaaagata tccggtcagc
     4441 acccgatatt gcattatttt tcgctgggcg ttttgccttt ttgcagcgtt tctttcacct
     4501 gaccgataag ctgacgacgg aaatccccga ggcgaggttt gtcatcgtca atccacggca
     4561 gtgggcggca aagctccata gctttaatcc ccagtcgtgc cgtcagaagt ccagcaccaa
     4621 ttccctgagc tgcgcgggta gacaaacgag cagcgagatc ttgcgacatc cagtccatcc
     4681 ccacttcgcg caccaattcg ctggctccgg cgaaagcgat attcagcaat accagcttga
     4741 acaggcgcaa acggctgtaa tatcccagtt caatgccata gagcgtggcg atgcgattaa
     4801 tcaaacgcag attgcgccag gcgataaatg ccatatccac caacgccagc gggctgaccg
     4861 caatcatcaa cgttgattca gctgctgagc ggctgatttc gcgccgcgcc tgggcatcta
     4921 aaaccggctg gaccagatga gcatacaggc tgaccacttc acgatcgttc tgcgtttcat
     4981 ggattgaggc ataccagcgt tgcagcgctg gatgagactg atcaataccc gcctgctgcg
     5041 ccagtttttc gcaaaatgcg cggcctttgc ccgtgccgtg gctgtgcaac aaatcgcgcg
     5101 cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa gcgccgccac tctgttacca
     5161 cagaacctac gccagcgccg ataatcaatg ccccagcggc acatccaccc agcgccaccc
     5221 agtcctgagt ttgccaggca ttcattgtcc actgtacacc ctgcccgaca acgcttgcgc
     5281 caaacagagc cagcccgccc atcaccattt tgcgccacag gctgcgtttc ggacgtaacg
     5341 ctgcgtccat taccgcttcg acttgcccct cttcttcctg cgcttcgtcg agcgtggccg
     5401 gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg cgccctgaat tttggattct
     5461 gttcgacctc cagaggaccg tcgaaatcaa tacgtggttt taacggttcg gtcatcgcaa
     5521 tttatctcct attaaaaatt ccagcgcggc atcaagacga atatgcggca gcggtttgtc
     5581 gacatccatc gcctgcgggc gaaacgcttc aaactggaac ccttgcttgt cccagaacgc
     5641 ctgaccgggc aaacgcgcag gaacttcacc ggggtaaacg gtgagaggcg caccatcgct
     5701 gagccggtta ccgcgcaatg ccggaatttt ctcgccgttg acgtcaataa tgccgctggt
     5761 ggtcgcctgc accgaggcca gccccaggca atccatacta atcccttcaa acgccgcatt
     5821 ttgccaggca tcctgaatca gttgttgcag taatgaaacc atattggcgt gctgatcgac
     5881 cgttacatgg tccgctttcg tcgcggcaaa cagcagtttg tcgataaccg gcgagaataa
     5941 gcggcgaaac agcgtgcgct gcccgtagtg gaaactttgc atcagctgcg tcagtgccag
     6001 acgcatatca ttaaatgcct gtggcccact gttgagaggt tgcaggcaat ccaccagcac
     6061 aatctggcgg tcaaagcgca gaaaatgatt cttatagaac cccttcacca ccttctcgca
     6121 gtaataatta aaccgctcgc gcagcattcc ggcattggtg tgcttatcgg cctgcgccag
     6181 tttggactcg ccccaggcat cgacatccgg ccacgggaag aattgcagcg cgggcgcacc
     6241 tgccatatcc cccggcaaga caaagcgccc aggctgaata aagtgcagcc cctgctgttt
     6301 acagtggtgg agataatcgg tccacgcggc ggcaatgtct gccagccggt tttcgtcggc
     6361 aggcgctagc gggtccagcc cttcgcacat cattcgccat ttcgccgacc attcgccgcg
     6421 ctgaccattg agtaagcccg tcatctggcg cgaccagctt aaatagtcct gcgccagcat
     6481 cggcaggtcg agcaaccatt cgccagggta atccacaatc tccagataca gcgtggaggt
     6541 atccttaaag tggcgcagca gcgaatcgtt cgatttatag cgtagcgcca ggcgaatttc
     6601 gctgacgccg cgcgttggcg tcggccaggc gggaggatcg ccatacagct gcgccaaccc
     6661 ttcgtcgtag gtaaaacgcg gaatgccaaa gtcacgctga ggaatgcgtt ttacgcccag
     6721 caggcgctct tcacgcaccg cacttaacag cggcaaacgt gctccggcat gaatattgag
     6781 caactgatta accatcgcgg tgataaacgc tgttttgccg ctgcggctaa gtccggttac
     6841 agcgaggcgc agatgtctgt cgacaccccg attcaccagc gcattgagtt catttttaag
     6901 tcgcttcatt gctgtcctgt gctgctctga agaccagaaa aaaatcgttc gtaaggatat
     6961 gaatgtgggg gccaggccat tgattatcaa ctgcccgtgt tgtcatcgac gggcgaactc
     7021 cataagatgt gcagcattca cgttcggacg gagttcctga cctatgtccc ctactattta
     7081 tgatattgcc agggttgcag gcgtatcaaa atccaccgta tcacgcgtgc tgaataagca
     7141 aactaatatc tccccggaag cgcgcgaaaa agtgttacgg gccattgaag aattacagta
     7201 tcaaccaaac aagctggccc gcgcgctgac ctcttcgggt tttgatgcca ttatggtgat
     7261 ttctacccgt tcgaccaaaa ctacggcggg taatccgttt ttctctgaag ttctacatgc
     7321 catcaccgcc aaagcggaag aagaaggttt cgacgtgata ttgcagacgt cgcacaacct
     7381 ggcagaagac ttacaaaaat gtgaaagcaa aattaagcag aaaatgatta aaggcattat
     7441 tatgctaagt tcgccagcgg atgagtcatt ttttgcccaa cttgataaat acgatattcc
     7501 tgtcgtggtg attggcaaag ttgaaggtca atatgcccat gtttattctg tcgataccga
     7561 taattttggt gacagcattg cgctaaccga tgcattaatt gagaatggac ataaaaacat
     7621 tgcctgcctt catgcaccgc ttgatgtcca tgtttcagtg gatcgggtaa atggttataa
     7681 gcaaagtctg tccgcgcata atattgcagt gcgtgatgaa tggattgttg acggcggtta
     7741 tacccatgaa acagcactgc aagccgcgag agaattatta agccagtcac cgttgcctga
     7801 ggcggtgttt gccactgaca gcctgaaatt aatgagcatt tatcgtgcgg cagcagagaa
     7861 aaatattgct attccgcagc agttagcggt ggtgggttat agcaatgaaa cgctgtcatt
     7921 tattttaacg cctgcaccgg gcggcatcga tgttccgacg caggagttag gtcaacaaag
     7981 ctgcgagtta ttattccgct taatttctgg aaaaccgtca ccacagaata ttaccgttgc
     8041 cacgcatatg acgctgaaat aaataccagc taccttgcct gaatgccgga tgcgatgcta
     8101 gcgcatctta tccggcctac atttatagat cggataaagt actcgcgccg catccggcaa
     8161 tttagctcat tatcagaacg agtaatttac gccgacacct gcataatgga atttatcgct
     8221 gtcgccttcg tcgtgatcct gccactcaaa cgcgtattcc agcgaaacgg aaagaccatt
     8281 ctggaaatca taaccgtaaa ataaaccaac acggttaaaa tcatggcctt cacgttcaat
     8341 atcatcctgc cagtcccagt tactccagcg atccagccca atgcgcgtat acggcgtcac
     8401 cgagtggttg ccgagcgtca gcggcaaata ggcacgaatt tcttgcgtgg aaaactcacc
     8461 gttattgcgg ctgtcgtcca tattgaagcc gcgctcgaga taatagttca ctcgcaaagc
     8521 aaccgtttcg ttaaaggtat attgcagacc tgtttccgtt tcgacacggg tatcagcgta
     8581 accggtcgtg ttcaaatcgt tggcaaattt atacatcgac aaccaaccgt tgaaacgtaa
     8641 atcgtcagtc agtttcacat cccagtctgg cgcgattttc cagcgctgca tattcgccgt
     8701 gtctttaccc ggttcatcaa cgtagtgata accataatta cggaaaccgc cggtcaggcc
     8761 gaaactgaaa tcatcgcttt cgaggaactg ataatgcacc tccagctccg ggcgatcaaa
     8821 ccacgttcca cgtttacccg cgctataatc taccggccct tcctgataat aggccagagc
     8881 aattctccac ggcccgttgg cggcattaaa atagacagaa ggctccgcca gtccatccat
     8941 atcttcgcca taaccctcga cgttttctat ttcgtacatc gcgccgatat taaagtgcca
     9001 gtcgttcttt tcctcggcct gtgcgcaggc cattcccgca cacatcacca gtgcggtaca
     9061 gggtaataac tttttcatta ttgttccttg tattatcctg ggggaatgcc ttccccctgt
     9121 atttagcgaa ttgctatttc cgtttctgca tcaaagaaat gacatttagt catatcaaaa
     9181 tgaatagtga tattttctcc tgcatgataa tcatttaacg caccagcacg gaccactaac
     9241 tcgtgccccc caaccgtggt gtagagcata aattccgcac cggttaattc tgccacgcta
     9301 attttggcgg aaatgttatt ttcctcttgc gcgtccggat gaatatcttc cgggcgtatt
     9361 cccatcacga tgggcttatg caaactttcc tgtgttttca gaaccgctaa tttctcttcg
     9421 ggaatggtta atttaagcgt ttccgtaacg aatttatcgc catcgatcgt gccgcgaata
     9481 aaattcatcg ctggtgatcc aataaatccg gcaacaaaca tattcgcggg ttggttataa
     9541 acggttttcg gcgcacctac ttgctgaaca atcccgtctt tcataatcac aatccgcgtc
     9601 gccatggtca tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt gttcagtttc
     9661 tgatgcagct tgctgatctc tgcgcgcatt tgtacgcgca gcttggcatc aaggttagag
     9721 agcggttcat ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca
     9781 cgctggcgtt gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg
     9841 agaatttgcg ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc
     9901 ttcaggccaa acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg
     9961 aacaccattg cgatattgcg tgctttggct ggaacgtcat tcattcgttt accgtcgatc
    10021 aacagatcgc cgccgctgat ctcctcaagc ccggcaatca tgcgaagggt ggtcgattta
    10081 ccgcagcccg acgggccgac aaatacgatg aactctttat cggcaatttc caggttgaag
    10141 tccttcacca catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga
    10201 gccatgttga ttcctttgct atacgttttg ccagaaggcc gataaccgcg gccaggtcag
    10261 ggagtccgtt gagggcaaca ataatcgcgc cccggttaag cctgcgccga tccccaccga
    10321 gcgcataccg ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca
    10381 tgcctgcggc ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt
    10441 cgagttttta agttgggaag catccgcgca gaaggtgaaa aactcgcgca gctccagcgc
    10501 cgctaaaatc gtcggcgcat tcagggagac ggaagccagc cctaccggaa tctgctgtgc
    10561 acggagctct gccagcaaat tgcgaatacc gggcagcaca gcattaaccg tcagctcgcg
    10621 cagtgagtgg acgtaaagca gatttttgcg atacgccagt tgcgtccgct cctgcgggtt
    10681 aaagtcgccc tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctaat
    10741 cccttttagg gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg
    10801 ctgccacgcc tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat
    10861 taccccttgc agtttcattc atcctcctga tgcgtggtag cggtcccatt gaagggcaaa
    10921 ataaaatcac ccaaacagaa aacagattct tcagccacgg atataagctg gccgttcaaa
    10981 cgcagtgaaa cgggcgcaga agttcgaatc gcaatacgct gcgcgtcgag agtgacctgt
    11041 aattcgcagc cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc
    11101 ggattgagat gcaattcacc gtcacgcacg ctcaccccgg caaaaccctg aatcgccccc
    11161 agccagatag cgccagttgc ggcagcgtgg ataccatcat cacaactatg cggatcagca
    11221 ccaagatcga tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag
    11281 cgtgcggcaa caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt
    11341 tcataaaatt gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa
    11401 ttgagcatca ccacatctgc ctgcttgagg atctgcatct cgttcacttc tgcgcgtgaa
    11461 taatccagca gtatggtttg cttccccgcc gccgctttgt atttcgccaa gttaatcgcc
    11521 ggcttagcca taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctgacagc
    11581 cgcagttctt taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac
    11641 tggcgggcaa tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgtatgca
    11701 ttattattga catgttcggt atattcgtct ggcccaataa catcatgaat ttccagacga
    11761 ttgttaactc tcaccgcgcg gctaatccag aatttcgcag tttccagcag tagcgccatg
    11821 ccttcgtgag cgatgaagct ttcatctccc gttgcctgcc agtattgaat gacagcccag
    11881 gcgatatcgg cgaccagatg atgttcagcc tgcgccgagg ccactttttg ccgcagtccg
    11941 gtgcggatgt taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctctcc
    12001 cacggaaaca gcgcgccctg ccaaccattg cgtcgcgctt tctcctgcgc gccaggcaag
    12061 ttgtgccagc gataacgcag taaacttcgg gccaccgtcg gatcgctaaa cagatggaac
    12121 ggtagcaaaa atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag
    12181 ccttttgccg caatactgct gcgctcgtcg tgagcgggcg tcatgatgcg cagatgataa
    12241 agcgcataat ctaacgcttg ctgatcgtgc gcatcgccgc cgtttaccgt gatacgacgt
    12301 ttctgccacc actgacgcca gttttctgtt gacgctgcaa gaagttggtc gtaactctgc
    12361 tgtgcgcaca tttcaagctg acgaagcgac gcgccacccc actcgtctaa agcggtttgt
    12421 cggtcatccc gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga
    12481 agctgcgcac tggtatgttg cagcaaacgg cgctctttgg cggtatagca ttgctgcaca
    12541 tcaccgctca ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg
    12601 tagatcccct gcatcagatg ctgaccaaac acccgcacct gggtttcgtc gagatgttgg
    12661 cgaccgtggt tggtttgcgt ggcgtcgata cctgttgaaa tcagcactga cgcgtcagcg
    12721 tccagtggcg taatagtgat ttccagcgca atgagcggca gttggtctgc cgaaacaaag
    12781 cgacggctgg cgatggtgta acgcgcgtcg ttgctggtac accagacaac gctgcgccgt
    12841 aattcgccac tggcaaagtc aagctcacgc tgccaggctt cgcgggataa tgagaaaacc
    12901 tcaccattta tgacaatctc catccccacc acatcgggca ggttcaccag ttcgttgatt
    12961 tcacctttac ccgcccgatg ataaagcccc gccagataca tgccgcgcgt ctggcgcgtg
    13021 taatcttctt catggctggc gcgaagccca agatagccgt tcccctgcgc catcagtgat
    13081 gcatacttgt tcagggtatg ctggctgaaa tggggttccg ttaatgttac tggcctggtc
    13141 ataattccac acgcgtccct gtttctgcgg attgatacag cgccgccacc aactgctgga
    13201 tgatgtatcc ctgctcggcg tcggcaatca tcatgggctt accttgtacg tggttgataa
    13261 aggcttccat acttcgcaga tggcggttgt catccgccgt ttcccgttgc atcagcgtca
    13321 ttaactcacc gttgttatcg gtatagatat gtgccggaaa cagcgtcgcc cctgctttat
    13381 cgccacaaaa gctgacgttc ataattgact gttcgcggat gttgagtgca aatgacgttt
    13441 ccagccacag aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc
    13501 tgtaggttgc cgggtcccac tcgccaaact gaccgcagct cttttgcgtg ccgatttttt
    13561 gaaaactgtg cgcagtcacg cttttcaccg ccggaaagcc cagcacatac atcgccgcat
    13621 ccagcatatg aataccgatg tcgataagcg gcccgccacc ctgcagttct ttattggtat
    13681 agacgcccca gccgggaaca ccgcagcgac gcagagcgcg ggcggtggtg acgtaaattt
    13741 cgcccaggat gccgttagtc acttgctcac gcagttgttg tgtatcaagc gcaaaacggt
    13801 gatggaagtc gtaggccagt acttttcctt gtctgcgcgc ggtatcgcgc atgacccgag
    13861 cctgttccgg cgtcatagca ggcggctttt cgcacatcac atggcagccg gcttccagtg
    13921 ccataagggt atgttcatag tgaaaacggt taggtgagca gacgctaacc acgtcagggt
    13981 tcaccgccag cagcatggcc tgcggatcgt cccacacgga tgcattaccg tatttatctg
    14041 ccagcgcctg cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc
    14101 gggtgcagta gtacgaagca tgaactttat ccgccacctg gcccgcgcct atgatcgcga
    14161 cccgcagcgg agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc
    14221 aggccaaaga atcacggtac gcctgggcag gatcttctgc ccgaatgcgc ccttcataca
    14281 ccacatagcc ctgataatta tcggcgcgca gttgttcaaa cagcgcgtga aaatccaggg
    14341 tgccgctgcc cggctgataa cgatggttat cggcaatatg cacatgaccg agcaggtcgc
    14401 ggttgtcatg cagcgcctgc gccaggttat cttcttcaat attcatgtga tagaaatcgc
    14461 cgataatctg cacatgttta agatcgtttt cgacgatgta acgacgcgca tcggcgagag
    14521 tgttgatcat gtgatcctga taacggttta acggttcgag atacaccacg gttccggtac
    14581 gtgctgccac ctgttccagt acgcgtaggg aatcactcac cattttgcgg tcgccgtcca
    14641 ggctgcgctg tgaagtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa
    14701 cgacgatacc tttaccgccc acttctgcca gcgcttcgag aatgcgctct atctgcttta
    14761 agccattaag acgacgctct tcgataaagt cgccaatcca gccgtcatag ccaccgcagg
    14821 cggtggtcac cggtaaacca gtttctttga tcgccgcttt cacttcttcg atattgttga
    14881 ccagcagttt gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt
    14941 tctccagaat attttccggg aagaacgcct gattctgtgt gcctattttc atttttccag
    15001 tcctttgaat taaaacgtga cgcccatttt gatgctctgt tccggatgct ggtcgacata
    15061 ctgcatgtag ctttccgggc tattagcaaa ggtcaccacc gggtcgatta aatcctcaca
    15121 gttgagataa ccgttcatca gcagctccca gcaggtttct tcgatacgct tgcggctcca
    15181 gcgcggataa tccgggttcg gttcgctgca cgcacgagag aagacaattt tggcgttatt
    15241 gaaatgcgct tcgcgtccga ggttgaaacc ttcggcaaac ggtttggcaa acgcgacata
    15301 ggagatggtg ccgccataag ccaggccgcg cagtgccgat tgcagcgcgt cggcatagcc
    15361 gctggtttcg ataatcacat cggcaccctg cttgccggtt agcgttttga tctctttacc
    15421 tacatcagtg ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca
    15481 gcgatgggcg ataggatcga cgccaatcac cacagacgcg ccagcgcgtt tcgccagttg
    15541 gatggcaatt tgaccaatcg cgccaagccc taccaccacg acaaaatccc ctacgcgcac
    15601 gttggcatcg cgaacgccac tcatggcgaa ctgcgccggg tcgtagcaga cggcgttttt
    15661 ccaggagctg ccttccggca ttttgcgcag cttgtagtta ttcactgcgt taatgatgac
    15721 cgtctcggaa agcggaccgt agccgcatac gctgtcgccc accgcgtagt cggtaactgc
    15781 gctgccgcat tcgataatgt cgccaaccac catgttgcca agctggaatt tgccaaactc
    15841 aatgccgcgc ggcgcatctg ccggacgcgg ggtgaacatc tgccattcac cgttaaagtc
    15901 ttcattgata aacgggctgg cggcgcggaa atcgaccact tccgttccgt gtttcggtgc
    15961 gccgaaacgc acgcggatct tcacttcatt ggctaaaatc gcccgatctt catattcaac
    16021 tagcgcagca acgcgaggtg ctgtggctac taactttttc atttaaaact ccttgtaaag
    16081 aaactggccg ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc
    16141 cgaaagcgca tacataatca ccaccggcaa tgcggtcacc agcgaggcgg ccatcatgcg
    16201 tccccagatg taatcaggcg tactgaacag cgcgttcagg cccacaggta aggtgaaatt
    16261 gctggcgctg gagaggaaaa tcgacgcaaa cagatagtcg ttccatgcca ccataaagca
    16321 gtagacgaac acggatatca gccccgacat cgccaatggc acggtgatac ggaagatgat
    16381 ttgcagacgg ttgaggccat ccatcatcgc cgcttcttca atttcatcag ggatggtgtc
    16441 gaagtagctt ttcagcataa acacggcggt gggcagggtt tgcgtcacca tggtgatgat
    16501 cagcgccatc tcagtgtcgt aaatgccaag cgcggtgatg attttgaaca gcggcaccac
    16561 cagcaaaatc ccggagaaca tatacaccgt gtaaaagctg gcgttgatgg tcattcgccc
    16621 tttaaagcgc aggcgggaaa gcgcgtaagc cccgagaatg ccgagaaata ccgctaccac
    16681 ggaggaaacc accgacacca ccatgctgtt acggaagtag tcgacaaacg ggaaaatcat
    16741 cgggttaaaa atgtcgacgt aatgctccag cgtccactgc tgtggcagca gcgtaggatg
    16801 cagcgagatc gcctcttttg cgctcttgaa cgaggtcatc agcatcacaa aaaacggaaa
    16861 cagcgtgatg atgagaaaca gcgccagccc gcagtaaaaa ccgatacgac tgagtgtgcg
    16921 tttatttgtt gccattgagg ttcacccgtt ttctggtcag cagaatgaca gcgaaaataa
    16981 tgatgaagag caccaccgag atcgccgccg ctttccctaa gtcattaaag gcaaaggcgg
    17041 ttttgtagag atagacacca agaatatcga ccttggttgt cagcagataa acgtcagcga
    17101 acatgtagaa catccagatg gtgcgcaggg tcaccaccgt cgccaggacc ggcataattg
    17161 cgggcagcgt gacgatgcga aaccgctgcc aggcattagc gccatccatc tctgccgctt
    17221 cgtacagcga tttgtcgatg gtctgcaaaa tcgccagaaa cgaaataaag gcatagggga
    17281 agtagcgcca gatggcgaac agcaccacca gcacaaagct actgcccgga ttatcgaacc
    17341 acagcggtgc ctgctcgtag agatgcagaa gatcgacgcc gaggtagttc acaatgccgt
    17401 agccgttgtt gaacatgtat ttccaggcga acaccagcga aatggacggc gttacgtagg
    17461 agaggatcac cagcgaacgc gcggttttgc gcaggcggaa ctcgcggttg aaaaacatcg
    17521 ccaccgccag cccgagcacg gtgctgcccg ccaccaccag cgcggtatac cagaccgtca
    17581 tccacagcga atgccagaag ccgggatcgg agaggatacg cacatagttg ctcaccccaa
    17641 caaacgttga ctcgatgttg ggattgagcg gcagacgtaa aaaactgatt tcgatattcg
    17701 acaccatcgg ccacgccacc agagcgccca gcagtaataa gctgggcgcg agaagcagca
    17761 gcgcaaaggg catatcggaa cgacctgaaa aaagcctgtt cattgcggat tcctgtcctg
    17821 ggtttagtgc tgttcgatca gttcatccag ttttttctga ctcgcttgca gcgtagtaga
    17881 gagatcggct ttacccacgg tgacgttatg caccattgaa ctcaccacgc cagaacccgt
    17941 cacatcaccc atgcgggtaa agtttttatc ccctactgcg ccaaaaacct gaatatttgg
    18001 cagttcacca attaactgat tcggcagttc acccagcgcc ttgatcacgt cgttgtcttt
    18061 ccaggtggcg gtagtgacaa ccgctttgtt caccggcagt gccgcgcctg gcgacatcat
    18121 cacccagtcg gcaatgttgt ccgcctgctc cataaaggtg acaaattttt cagccgcttc
    18181 ggtctcttcg gtcttttgcc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc
    18241 agagtttttc tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac
    18301 agccggaagg atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc
    18361 tttcacttcc atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata
    18421 ggtcagcgcc tgcatcatct ctggtgaatc aagggtgatt ttgccttcgg cgttaaagac
    18481 gttggcctgg ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc
    18541 cgtaggcagc gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc
    18601 cagcagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg
    18661 ataccagatg ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc
    18721 ggtacgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac
    18781 cgtggcaacc gccttgcgat cgataagctg ctctttgtcc ataactttgg cgtagtcatg
    18841 actggtttcg atcacttccg gcagcgagcc gctacgtgaa agagtgatga ctttggtgtt
    18901 ataagcgtcc tcttcgacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa
    18961 acgggcaatc aatttactga taacgacctg acgctcctgc tccaccgaag aatgcataaa
    19021 ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac
    19081 tagtgctgat aacagcacga tttttgattt aatcatgaaa tgttcctttt ttatttaatc
    19141 cacataacct gccaggggcg cagcgttatt tcattaccca atatatcaac ttcactaatc
    19201 agatcccgac cgtgcaggcc agtaatattt acatgctgaa tatttttact gacattaaac
    19261 aatccagtca gacaattacc ctctgcgtta cttctttgaa tacgcattac ggatgaatta
    19321 accgtatcga tggtaaaatt attatccgga tgaaactcgt tatggctacg acgaagtata
    19381 attaaacgcg acaactcatg atataccgca tgccttaatg tagcttcatc gttcagttct
    19441 cgggttatct cttctctgta atatttttta cggttaatcg cacggttata tccgagtttt
    19501 tcgacacctg cgtaatcatt acgcgagccc agaatacttt gaatatatat cgctggcaca
    19561 ccggggaaac ttaacaaaat cgcatgggca aggataaacc tggcgcaacg ttcttcatcg
    19621 ctactctcgc ggcggcttaa cgcatccata taggtgacat ttatttcata gggactgcgc
    19681 gtaccgtcgg gattattttt ccagtttact aatgcccctt cctgctgtaa tgcctcgacc
    19741 agcgctaata tttcgctttc tggtaacaag ccccgcagtg ggttcagccc gatgccatcg
    19801 tgagaggcga ggaagttaaa ccaggtcgtt ttaccggagg gcaagctcag actttgcgcc
    19861 cactggcaca gcgcctcgac gttctgtttt tgcaccgcat gtaacaccag cggcggcagc
    19921 gagaactggt acaccatatg cgcttcgtca tcgccttcgc caaagtaagc aatgttatct
    19981 ttatgcggaa cattcgtctc agtaataatc accgtacctg gcgcgatgtc atcaataatc
    20041 gaccgtaaca gtttgataat cagatgtgtt ttttccagat ggatgcagct tgttcccggc
    20101 tctttccaca taaagccaac ggcatccaga cggacatatt ctgcgccctt ttccaggtaa
    20161 caaagcaaaa catccaccat cgccagcaac acgtcagggc tacggtaatt caggtcaatt
    20221 tgatcgtcgc taaaggtggt ccataaatgg cgcgtggaat tatcatccat ttgaaacggc
    20281 gttaatagcg gtaatgcacg cggacgggtg acagagctga gatcggtttg cggatcaacc
    20341 gcaataaaaa aatcttcaaa acctggctgc tgttgtaaat agtttttaaa ccattcactt
    20401 tttgccgaca tatggttgca gacaaaatca aacattaaat gactgcattc accgagctgc
    20461 tgaatatcct gccactcccc cgcttcactg gcgacctgat gataatcaat taccgaaaat
    20521 ccatcatcag atgaccatgg ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc
    20581 agccattgaa ggtaaaactg attaaatgtg ggtagtggtt ttaaatcatt actgtgaaat
    20641 tgatcggcat aggtaattaa cacgacatca ctttcatccc agtgtttttt acgtcgctgt
    20701 gtaattaatc gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg agttgaggta
    20761 aatgttccac cgtagatttt gtccaggtaa tccgtaattt tctgcttcat gttgcctttc
    20821 cgtggtagcg gtcccatgaa atgagagact actccgcaga aaataaactg tcaattgagc
    20881 acaagggttt tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc
    20941 tgacgcaaat aatctatggg gatgtaaata aggtgtgaca gaaaaaaacg gcgcataagc
    21001 gccgctcatg gtgaattctt ttaacctttg accttcggca ttgcgatgtc tttcaggcca
    21061 ccggcgttct caacgtgggt atatcccatc tcgctaagga tctcttttgc ctgccctgac
    21121 tggcgtccgg cattgcaata cactttcacg gtgtcgtttt tatcgggaac ggcggtggca
    21181 atgcgctctt tcacttcctt caggggaata ttgatggccc cctgaacgtg ctcttgctga
    21241 tactgctctg gaacacgaac atcgatccag tgttcagcgg caaaaacggg cagtgaaaac
    21301 accagtgcca gagcaagtaa gcctttttta aacatgtaag cgtccttcta tggatgaaaa
    21361 ttgccatcca ttataggcct gagatttaac aatttttgcg aaactcccct caccttttat
    21421 aacgctgtgc cagtttatta gcagcccgac ttaacagcgg ttccagcgcc acagccagca
    21481 acattttcag cggtcggcga gcaactgatt ttatcgccca gcccgccaca cccgccgggc
    21541 catagcgcag tgcggtaaga agtaccagct tgcctgctaa tttgaaacca ggcttcacct
    21601 tttgcccggc ctgttgccag cgagtattca tgactttcct cacagttgac ggaaacggct
    21661 acgtaacgtg aaagtatcgg aagtgacata acgttccatc tcgcgtaaac gcgtttcact
    21721 tgccgccagt tcacggtcga cttcatccag caattcgctg ctggaaggta gctgctcacc
    21781 aaaggccatg ttgtccggca ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag
    21841 ggtaaacagc gccagaccga agaaaatcga cagcaccacc aggattcgca ccagttttac
    21901 cggtacatca aaatagttgg caatcccggc gcagacgcca cggaccatgc cctgctgtgg
    21961 aatacgccat aattttttat tgagattaat gcccgccatt agcgatccct ccagttcgga
    22021 tgttctgcat caagaataga ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca
    22081 tcagccagtt gcgctaatcg ctgctgctca ctttgcgaca attcactgcg accagaacga
    22141 ttgctgtaat gcagccataa ccagatcggt aaaacaaaca gcacaaaaat ggttaatgga
    22201 atagccagaa atagcgcgct cataagtact ccttagacga tgagcggcgg cgctttcgga
    22261 cgccgcctgg atgttattat tgattgtctt gcttcatttt ggcttttaat tgtgccagtt
    22321 gctcgcttat tgcatcatcg gctttcagtt cggcaaactg atcgtccagc gatttttgtt
    22381 taccgaagct gtggctttct gcttccgctt ccatctggtc aatacgacgt tcgaaagatt
    22441 cgaaacgagc cattgcttca tccagtttgc cactgtccag ctgacgacgc acatcgcgcg
    22501 acgagtttgc ggcctgatgg cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca
    22561 atttgttttc cagctcgcca atctctttct tcatgcgtgc cagcgtatcg tccaccagcg
    22621 tcacttcatg ttccagggac ttaatcagat cggtcagttt ctgtttttca attaacgccg
    22681 cacgcgccaa atcctctctc tctttcagca gcgccagttc ggctttttcc tgccattcaa
    22741 cttcacgcgc cgacgcttgt tcaatacggc gagtcagctg tttcttttct gccagcgcac
    22801 gcgccgaagt agaacgtact tcaaccaatg tatcttccat ctcctggatc atcagacgca
    22861 ccagtttctg tggatcttcc gctttctcta acagagcgtt gatgttggca ttcacgatgt
    22921 cggcaaagcg agaaaaaata cccataatgt tgtcctcttg atttctgcgt tcaggattgt
    22981 cctgctgaac agttaataca atttgcgtgc caatttttta tctttttgat ttataaagat
    23041 ctgattgaag aatcaacagc aacatgccag gatgagttag cgaattacac taacaagtgg
    23101 cgaatttcat catggcagaa tacaaagata atttactcgg tgaggcgaac agctttctcg
    23161 aagtgctgga acaggtttcg catctcgcgc cgctggacaa acctgtgctc atcatcggcg
    23221 agcgcggcac cggtaaagag ctgatcgcca gccgcctgca ttatctctcc tcccgttggc
    23281 aagggccgtt tatttccctt aactgcgcgg cgttaaatga aaatctgctg gattccgaac
    23341 tgtttggtca cgaagcgggg gcgtttaccg gtgcgcaaaa acgtcatcca ggaagatttg
    23401 aacgtgccga cggcggtacg ctatttcttg atgaactcgc tacagcaccg atgatggtac
    23461 aggaaaaatt attgcgcgtg attgagtacg gtgaactgga gcgcgttggc ggcagccagc
    23521 cgttgcaggt gaatgtgcgg ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca
    23581 atgaaggtac ttttcgcgct gacctgctcg accgcctggc ttttgatgtt gtgcaactgc
    23641 cgcccctgcg cgagcgcgaa agcgacatta tgctgatggc agaacacttt gccattcaga
    23701 tgtgtcggga aatcaagctg cctctgttcc cggggtttac ggagcgcgcc agagaaacat
    23761 tgctgaatta tcgctggccg ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag
    23821 tgtatcgcca cggcaccagc gattatccgc ttgatgacat cattattgat ccctttaaac
    23881 ggcgtccgcc tgaagacgct atcgccgttt cagaaaccac ctcgcttcca acactgccgc
    23941 tggatttacg tgagtttcag atgcagcagg aaaaagagtt gctgcaactc agtttgcaac
    24001 aagggaaata taaccagaag cgcgcggctg aattactggg gttaacctat catcagttcc
    24061 gcgcgttgtt gaaaaagcac cagatttagc gaagttgggc aatacaactt gccggatgcg
    24121 gcgtgaatgc tttatccggc atacataatc aatacgtcgt ttgcagatct gataagcgca
    24181 gcgcatcaga cattattgcg ttgggctatt aatcgctcag cgcatcctgc aaaattttca
    24241 ttgccgcatc gaattgcgca tccgggatgg tcagcggata caggaaacga atcacgttgc
    24301 cgtatgcgcc acaggtcagt agaagcagtc cctgcgccag cgcgcgttgc tggattttct
    24361 gtgcaatcgc cgctgacggc tcgcccgttt gcggatcgtt aaactctacc gcaatcatag
    24421 accccagacc gcgtaccgcc gcgatggccg gaacgctttc tttggcatca atcagcgtgt
    24481 ttgtcagacg ctggcccagt tgattcgcgc gttcgcagag tgattctttg tcgataatgt
    24541 tgagcaccgc gtgcgcggca gccaccgcca gcgggttacc ggcgtaggtg ccgccaagcc
    24601 cgcccggcgc gggtgcgtcc ataatattcg cgttaccgac cacgcccgaa agcggcatcc
    24661 cgcccgcgag gcttttcgcc atcgtcatta aatccggctt atcggcgtaa tgatccatgg
    24721 caaacagctt accggtacgc gcaaagccgc tttgcacttc atcagcaatc atcacaatac
    24781 cgtgctcgtc gcacaggcgg cgaatggcgg caaccagctc ttttggcgca acgttgaaac
    24841 cgccctcgcc ctgcaccggt tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg
    24901 atttaaacaa gcgttcgatg gcgtcgaggg agtcctgtgt tgaaacgcca tgtaaatctg
    24961 acggataagc tacgtgatac accgaaccgg ggaacgggcc gaagccaatt ttgtacggcg
    25021 cgacttttcc ggtcagcgcc atggtcatat acgtgcgacc gtggaagccg ccactaaatg
    25081 caatcacgcc agggcgtccg gtatgggcgc gagcaatttt caccgcgttc tcaaccgctt
    25141 ccgcgccggt agtgaagaac gcggttttgg cctgcccgct caccggggca agggcgttga
    25201 ttttctccgc aagggtgacg tagctttcat acggcacaat ctggtacgcg gtgtgggtaa
    25261 actgttgcag ttgctgctcc accgccgcga ccaattcagg gtggcgatgc cccgtgttca
    25321 gcaccgcaat gcctgcggcg aaatcgatgt actcgttgcc ctcaacatcc ttcagcgtgg
    25381 cgttttcagc cgactgggcg aagaagttac acatcacgcc aaccccgcgc ggggtggcag
    25441 aaagacgacg ctgatggaat tcattgttgc tcatgtttca caccttttga ctcttcactg
    25501 ttaatcggaa attaaacgcc cagacgatcg cgcaggctgt aataagccgc gcccatcgcg
    25561 gtaaacggta cgcgcagcgt gcgcccgccg gggaacgggt aatgcggcag attggcgaag
    25621 gcatcgaaac gttcggcgtc gccgcgcagc agttcggcaa tcaaacgtcc ggcaagatga
    25681 gtacaggtga cgccgtggcc gctgtaaccc tgcatgtaat agatgttggt atcgaggcga
    25741 ccgaactgcg gcatacgcga cagggtcagc aggaagttgc ccgtccagcg atagtcgatt
    25801 ttcacgccct tcaactgcgg gaaggttttc agcagtttcg gcaccacaag acgctcaacg
    25861 tcatccgggt cgcgcgcgcc gtacaccacg ccgccgccgt acagcaaacg gttgtcggcg
    25921 gtaagacggt agtaatcaag cagatagtta cagtcttcca cacagtagtt tttcgggatc
    25981 agcgaacggg ctaaatcttc tgacagccgc tcagtggtga tcacctgcgt gccgcacggc
    26041 atactacgct tcgccagttc cggctccact ttatcgccca ggtacgcatt cccggcgaca
    26101 atcacatact tcgccgtcac ctggcctttg gtagtacgca ccacggcggg cgtggtgtgc
    26161 tggatctgcg tcaccgcaga aagctcatac acacgtccgc cgttgagacg aatggcatcg
    26221 gcttcaccaa tggcgaggtt cagcgggtga atatgcccgc cgctgtgatc cagcagcgca
    26281 ccagtgtagc gatcgctggc gacctcgcgg cgaatggcgt tggcgtccag catctccagc
    26341 tgtttattgc cgtagcgttc ccagttctct ttctgctctt caagtgtggc gagctgttta
    26401 tcattcatcg ccacaaacag accgccgggg cggtagtcgc agtcaatctg ataacgtttg
    26461 atacgttcgc ggatgatctc accaccttcg aacatcatgc tgccgagcat acgggcggtg
    26521 tccatgccgt agcttttttc gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc
    26581 ccgccgttgc gtccgcttgc gccgaagcca atgcgtgagg cttcaagaac caccacgtca
    26641 aagcccgctt ccgccagatg tagcgcggaa gacagcccgg tatagccgcc gccgaccacg
    26701 caaacgtcgc aggtgattga ctcattcagc gtgtcgaatg gcgtgtattt attcgcactg
    26761 gcggcgtagt aactgctggt atgttcggtc atttcaggcc tccaggctta tccagatggt
    26821 tttcagttcg gtgaattttt caagggcatg cagggatttg tcgcgaccat tgccgctctg
    26881 cttatagccg ccaaacggca cggtcatatc gccgtcgttg tagttattga cgaagacgga
    26941 accggccttc aggcggcggc tcatacggtg cgcgcgggag agatcgcgcg tccataccgc
    27001 cgcgccgagg ccatactggc tgtcgttggc aagctgtagc gcctggtctt ctgatttgaa
    27061 acgcgtgacc accagcaccg ggccgaaaat ctcttcgcga cttaaggacg cattcgggtc
    27121 tacatccaca aagatggtcg ggccgatggc ggcagccagc tcggcgttac ggccatccag
    27181 cagcagttgc cctttgcttt cgccttcccg aataaagcta tggaccgagt cggcgtgggc
    27241 gcagtcgatt aaggtgccca tggtggttgc ggggtcgagc ggatgccccg gctgccagtt
    27301 ttgcgcctgc tgctttaaca ggtctaagaa ttcatcggcg atgctctctt ccagtaacaa
    27361 acgcgttccg gcgatgcaca cctgtccctg gttgtagaaa atgcctgcgg cggtggcgct
    27421 cgccgctttt tgcagatccg ggcagtcggc gaaaacgatg ttggcgcttt tgccgcctgc
    27481 ttccagccag acgcgtttca tgttgctgtc gcctgcatct ttcagcagtt gtttcccggt
    27541 gcgggttgaa ccggtaaagg cgatggcgtc gatatcgtta tggcgcgaca gcgcctgccc
    27601 cgcttcatga ccaaaccccg tcaccacgtt caacacacca tccggcaagc ctgcttcttt
    27661 cgccagcccg gcgagacgaa tcgcactgag cggtgatttt tcagacggtt ttagaatcac
    27721 gctgtttccc gccgccagcg ccgggccgag tttccagcaa gtcagcagca gcgggaagtt
    27781 ccacggcacg atggcggcaa ttacgccgac cggttcacgc acgatcatcg ccagctcatg
    27841 gctactggtg gtcgccactt cgccatacac tttgtcgatc gcttcggcgt accagcgaat
    27901 ggcgcgcgcc gcgccgggaa tatcatcacg cagactgtga cgaatcggtt tgccggtgtc
    27961 gagagtttcc agcagtgcca gctcttcggc gttggcttcc attaaatcgg cgagtttatt
    28021 cagtaccgct ttacgtttag ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc
    28081 gcgtgctgcg ctcaccgcac ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag
    28141 cggtgcctgg gtgaccggat caacggtttc aaaggtttca ttttccgccg cagcagtata
    28201 ttcaccgtta ataaataagc gggtttcaat ggcgagactt aacgctttat cctgccagta
    28261 agccagatga tgaaaattca ttatgactcc tgtttcacgt ctatcagata tatgcagttt
    28321 gtgaacgtcg gggcgcagag atccccgggc cgtatgcccg tttatttctg gcgacattct
    28381 ttatggattg ccgggcgcat tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg
    28441 gtgggcgtat gggcgctgat aattcggcaa atacctgccg acgtattact gaaactgtgc
    28501 gggatgccgg tattaatggc ataactttgc cccgcgacga ggtggtaatc ctgaccatta
    28561 atcgtcagaa caatttcacc ttccagtaca gtgcctattt cctcaccctg atgcttaatt
    28621 ctttccccag tggttgtgcc cggctggtac gtttcaaaga tcatcgccag cgtgcgattc
    28681 gggttaccgt tatgaaccag cttcattgac acaccctgac tgcccatctc aattaaatcg
    28741 tcctgattaa tgacgacctg cggctcatca ggtttttccg gctcggaaaa gaattccgag
    28801 agtgacagac catacacctt cagcagcttt tgcagcgtac tgatggcagg gctgacttta
    28861 tcttgttcta tcgtactgat agcactgtga gtcagcccgg agagttcggc ggcacgacgt
    28921 tgtgaaagcc cctgctgctg gcggatttcc gacaagcgtt ttcctggcgc cagtccctca
    28981 tcactcatat ttgcatttcc ttaaactgta gtggtcagag tcgctgtttt tcagcgatat
    29041 ggtgctgaca agcggtgata aagccctcga acaatatacg cgaaagcgcg tactcgctac
    29101 tgttccattc cgggtgccac tgtacgccca gcgcaaaagg atgattgatg acgctaaccg
    29161 cctcaactaa gccatccggc gagcgagctt caacacgcaa ccgtgggcta acgaccttcg
    29221 ccccttgtcc atgcagagag tttacccaaa agttgctaca ttcaggtaac aacgcagaca
    29281 gtaatccccc ctcttcaacc tgaacttcat gcgacggtgc atattgctgt tccaccggca
    29341 gttcgggatc ttcccgatgt tccagcaatt caggctgttc gcacagcttg cgatgcaacg
    29401 aaccaccggt tgccaccacc agttcctgta aaccccggca gatggcgaaa atggggatgc
    29461 gcctttcgag tgcggcatta attaacgcca tgctcagaag atcacgcccg ggatcggcgt
    29521 caggctcatc gccgttttca ccatataggt gcggctgcac attgctggga ctaccaggaa
    29581 gataaatgcc atcgagtttc ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat
    29641 gtggcagcgc aattggcaag ccgcctgcgt ggatgatggc attcaggtac ttttcttgca
    29701 gagtctgggt cgcatgaccc ttaagcctgt tcctgcacat tacgacaccg ataaccggat
    29761 tgttcattat attttccatg ctcaatctca caaagtggac taaattatcg ccattactgc
    29821 ggtttaaatg acctttatgt tcaatatttt ttcaatctag cagtgggttt tcatttttgc
    29881 aaactcaatt taacatttga caaacattta gtttgcatac agattcgaat ggtggtcatt
    29941 atattttacg ctttgataac gagcggaaaa caaaccaaag gcgaagaatc atggaaacca
    30001 atatcgttga agtagagaac tttgttcagc agtcagaaga gaggcggggt agcgccttta
    30061 cgcaggaagt gaaacgctac ctggagcgct acccgaatac gcaatatgtt gatgttttac
    30121 ttaccgattt gaatggttgc ttccgtggaa aacgtattcc tgtttcaagc ctgaagaagc
    30181 tcgagaaagg atgttatttc ccggcctcgg tgtttgcaat ggatattctg ggcaacgtag
    30241 tagaagaagc aggtctgggt caggaaatgg gcgagccgga tcgtacctgt gttcctgttc
    30301 tcggttcctt aactccttct gccgccgatc cagagtttat cggtcagatg ctcctgacca
    30361 tggtcgatga agatggcgct ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt
    30421 ggcagcagct gcgccagcgc ggattgttcc cggtcgtagc ggtagagctg gagttctatt
    30481 tactggatcg ccagcgcgac gctgaagggt atctgcaacc gccctgtgca cccggcaccg
    30541 atgaccgcaa tacacaaagc caggtttact ccgttgataa cctcaaccac ttcgccgacg
    30601 tgctcaatga tattgatgaa ctggcgcagt tacagctgat tccggcagat ggcgcggtcg
    30661 ctgaggcctc gccgggtcag tttgaaatca atctttacca taccgataac gtgctggaag
    30721 cctgcgatga tgcgctggca ctaaaacgcc tggtgcgtct gatggcagaa aagcataaga
    30781 tgcacgccac ttttatggcg aagccgtatg aagagcacgt gggcagcgga atgcatatcc
    30841 atatcagtat gcaaaataat cgtggcgaga acgtgctttc tgacacagaa ggcgaagatt
    30901 cgccgctgct gaaaaagatg ctcgccggga tgattgacct gatgccgtca tcaatggcgt
    30961 tgctggcacc aaacgtgaac tcgtatcgcc gcttccagcc aggtatgtat gtaccgacgc
    31021 aggcgtcgtg gggccataat aaccgtaccg tcgccctgcg tattccatgc ggcgatcgcc
    31081 ataatcaccg cgtggaatat cgcgtggcgg gtgccgatgc caacccatat ctggtgatgg
    31141 cagcgatttt tgccggtatt ttgcacggtc ttgataacga gctgccgttg caggaagaag
    31201 tcgaaggcaa cgggctggaa caggatggct tacccttccc gattcgccag agcgatgccc
    31261 tgggtgagtt tatcgagaat gatcacctgc gccgctattt aggcgaacgg ttttgccatg
    31321 tgtatcacgc ctgtaaaaat gatgagctgt tgcagtttga gcgcctcatc acagaaacag
    31381 aaattgagtg gatgttgaaa aacgcatagt accccttgcg gggttgtttc atcggcctga
    31441 gcggagccag gcctgacatt tgctggtaat ccagacattt cccggatgtc gcgttatgga
    31501 gcgcaggcgg caacgggctt ccgttaacga ttaagactgt gacgaggaaa tgagatgatg
    31561 ccgatgatta aatccccgca tggcgcggga ggctgcgtat gcgcccctcc tgcgactgac
    31621 tggactccgc cccctttgct tcccttgcta aacaggtttg atttcaggtc gacacgaccg
    31681 caaaccttat tacgcagggg aggcagcaat tatggctatt aattcaccac tgaatattgc
    31741 tgcgcaaccc ggcaaaaccc gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat
    31801 gggtctggcc tatctcacgc cgatgaccgt atttgatact tttggtattg tctccggcat
    31861 tagcgacggt cacgttccgg cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc
    31921 tatcagctac ggcaaactgg ttcgccagtt tccggaggcc ggttcggcct atacctacgc
    31981 gcaaaagtcg attaacccgc acgtcggatt tatggtcggc tggtcatcac tgctggatta
    32041 tctctttttg ccgatgatca acgttttgtt ggcgaaaatc tatctctccg ccctcttccc
    32101 ggaagtgccg ccgtgggtgt gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct
    32161 gaagagcgtc aacctggtcg ctaacttcaa taccctgttt gtactggtgc aaatctccat
    32221 catgctggtg tttatcttcc tggtggttca gggactgcat aaaggagaag gcgttggcac
    32281 cgtctggtca cttcagccgt ttatcagcga gaacgcgcac ctgatcccga ttattaccgg
    32341 ggcgacgatt gtctgtttct cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga
    32401 gacaccggac gccgcacgtg tgatcccgaa agccatcttc ctgacggcgg tctacggtgg
    32461 cgttatcttt atcgcggcgt cgttctttat gcagctgttc tttcccgata tcagccgctt
    32521 taaagatccg gatgcagcac tgcctgaaat tgcgctgtac gtcggcggca agctcttcca
    32581 gtcgattttc ctctgcacca cgtttgtgaa cacgctagcg tctggcctgg cttcacatgc
    32641 cagcgtgtcg cgtctgctgt atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt
    32701 tggctatgtg cacccaaaat ggcgtacccc ggctctgaac gtcattatgg tcggtattgt
    32761 cgcgatgtcg gcgctgttct tcgatttagt taccgccaca gcgttaatta acttcggcgc
    32821 gttggtagcg tttaccttcg ttaacctgtc ggtgttcaac cacttctggc ggcgtaaagg
    32881 aatgaataaa agctggaagg aacacttcca ctatttatta atgccgctgg ttggcgcgct
    32941 gacggtgggc gtgctgtgga ttaacctcga agccacctcg ctgacgctgg gtctggtatg
    33001 ggcttcgtta ggcggcgcat atttgtggta tctgatccgc cgctatcgca aagtgccgtt
    33061 gtatgatggt gacagaacgc cagtgagaga aacgtaatac gtaggatgcg cggcgcatta
    33121 tcctgcctga gggtaatgca ccgcgccatt aactataaaa agatgtatga cccggatcac
    33181 gagtgctatc atccgccctt atgcttagcg cattaactcc aggggcaaac aatgaaccac
    33241 gatatcccat taaaatattt cgatattgcc gatgaatacg cgactgaatg cgcagatcct
    33301 gtcgcagagg ctgaacgcac accgctggcc cactacttcc agttactgct cacccgtttg
    33361 atgaacaatg aagagatcag cgaagaagcc caacatgaaa tggctgctga agcggggatt
    33421 aatcccgtac gcattgatga gatcgcagag tttttgaatc aatggggtaa tgagtaatta
    33481 tttaattgca gacgatcggt cagtatcgtt gtccggataa agcgttcact ctgcatccgg
    33541 ctaacaatga cacgcactgc agcgtgcttt aatgggtttc tcacgatcgt acctctctgc
    33601 tcctcgctaa gttgttctgt gagttatagt tatagagaaa tcacgggtac gaatgttgaa
    33661 cagatggcga cgccacctgt tcaacgacca accacacggc cctcattacg acccctgaac
    33721 acaaacggta atggcaaatg tcattaaata ttagtaagat agcctcttta cataaaatcc
    33781 cttaatatta tgcgccaggt attatcgtct cttttggtga ttgctggact tgtgagtggt
    33841 caggcaatag ccgcgcctga acccaccccg catgctgata tccgcgacag cggttttgtc
    33901 tattgcgtca gcgggcaagt caacaccttt aacccatcca aagcgagcag tgggttaatt
    33961 gtcgataccc ttgccgccca gttttatgat cgactgctgg atgtcgatcc ctatacctat
    34021 cgcctgatgc cggaacttgc cgaaagctgg gaagtactcg acaacggcgc gacctatcgc
    34081 ttccacctgc gtcgcgatgt tccgtttcaa aaaaccgact ggtttactcc cactcgtaaa
    34141 atgaatgccg acgatgtggt gtttaccttc cagcgaattt ttgaccgcaa caacccgtgg
    34201 cataacgtca acggcagtaa ctttccctac ttcgacagtc tgcaatttgc cgataacgtg
    34261 aaaagcgtcc gtaaactgga taatcatacc gttgagttcc gactcgccca gccggatgct
    34321 tcttttttgt ggcacctcgc aacccattat gcttcggtca tgtcggcaga atatgcccgg
    34381 aagttagaga aagaagatcg ccaggagcaa ctcgaccgtc aaccggtcgg caccggtccg
    34441 tatcaattgt cggaataccg cgccgggcaa tttattcgcc tacaacgtca tgatgacttc
    34501 tggcgcggta aaccgttaat gccgcaggta gtggtggatt taggctccgg cggcaccgga
    34561 cgtctgtcga aactcctgac tggtgaatgc gacgttctgg cctggcctgc tgccagccag
    34621 ctatccattt tgcgtgacga cccgcgcttg cgtttaacgc tgcgtcctgg gatgaacgtc
    34681 gcctatctgg catttaacac cgccaaaccg ccgctaaata atccggctgt ccgccatgct
    34741 ctggcgttgg ctatcaataa ccagcgcctg atgcaatcca tctattatgg tacggctgaa
    34801 actgcggctt ctattttacc gcgcgcctcg tgggcttatg acaacgaggc taaaattact
    34861 gaatacaatc cggcgaaatc gcgcgaacaa ctgaaagcgt tggggctgga aaacttaacg
    34921 ctgaaactgt gggtgccaac acgttcgcag gcgtggaacc ccagtccact gaaaactgcc
    34981 gaactgattc aggcggatat ggcgcaggtt ggcgtaaaag tggtgattgt gccggtagaa
    35041 ggtcgctttc aggaggcgcg gttgatggat atgagccatg atctgacgtt atccggttgg
    35101 gcgacggaca gtaacgaccc ggacagtttc ttccgtccgt tactgagctg cgcggcaatt
    35161 cattcacaga ccaacctcgc ccactggtgc gatccgaaat tcgacagcgt attgcgtaag
    35221 gcgctctcct cgcagcaact ggcggcgcgc attgaagcct atgacgaagc gcagagtatt
    35281 ctggcgcagg aattgcccat tttaccgctg gcgtcgtcat tgcgtttgca ggcctatcgg
    35341 tacgatatca aaggtctggt acttagcccg tttggtaacg cctcctttgc tggggtgtat
    35401 cgcgagaaac aggatgaggt gaaaaaacca tgattatctt caccttacgc cgcattttgt
    35461 tattgattgt caccttgttc ctgctgacct tcgttggctt cagcttgagc tatttcactc
    35521 cacacgcgcc gttacaaggc gcgtcactgt ggaatgcctg ggtgttctgg tttaatggcc
    35581 tgatccactg ggattttggt gtttccagca ttaacggtca accaattgcc gaacagttaa
    35641 aagaggtctt cccggcgacg atggaactgt gcatcctcgc cttcggtttt gcgctgattg
    35701 ttggcatccc cgtggggatg attgccggga ttacgcgcca taagtggcag gataacttga
    35761 tcaacgccat cgccttactg ggcttttcaa tcccggtgtt ctggctggcg cttctgttga
    35821 cactgttttg ttcactcacg ctaggttggt tgcccgtttc agggcgtttc gatctgctct
    35881 acgaagtgaa accgattacc ggttttgcgt tgattgatgc ctggctttcg gactcaccat
    35941 ggcgggatga aatgatcatg agcgcaatcc gccatatgat attgcccgtg atcaccctgt
    36001 cggttgcgcc aacaactgaa gtgatccgcc tgatgcgtat cagcactatt gaagtgtatg
    36061 accagaacta tgtaaaagcg gcggcgaccc gcggtttgtc gcgctttact attttgcgtc
    36121 gccacgttct gcataacgcg ttaccgccgg ttattcctcg cctgggctta cagttttcca
    36181 ccatgctgac gctggcgatg atcaccgaaa tggtttttag ctggccgggt ttagggcgct
    36241 ggttaattaa cgccatccgc cagcaggatt acgcagccat ttccgccgga gtgatggtgt
    36301 gtggctcact ggtcattatt gttaacgtga tttctgatat tttgggtgcc atggctaacc
    36361 ctctgaaaca taaggaatgg tatgccttac gatagcgtat acagcgaaaa gcgcccgccg
    36421 ggcacgctgc gtaccgcctg gcgcaaattt tatagtgacg cctctgcgat ggtcggcctg
    36481 tacggctgcg cggggctggc tgtactgtgt atttttggcg gctggtttgc gccttacggc
    36541 atcgaccagc aatttctcgg ttatcaatta ttgccgccat catggtcgcg ctatggcgaa
    36601 gtttctttct tcctggggac tgacgacctc ggacgcgatg ttttaagccg tttactgagc
    36661 ggagctgcgc caaccgtggg tggcgcattt gtggtgacac ttgccgcgac gatctgtggc
    36721 ctggtactgg gcaccttcgc cggggcgaca cacggcctcc gctcagcggt gcttaaccat
    36781 attctggata ccttactggc gatcccttcc ctgctgttgg caattatcgt tgtagcgttt
    36841 gccggaccga gtttgtccca cgccatgttt gctgtctggc tggcattgct gccgcgtatg
    36901 gtacgttcga tttacagcat ggtgcatgac gaactggaaa aagagtacgt tatcgccgcc
    36961 cgtctggatg gcgcatcaac gctgaatatt ctctggtttg ctgtgatgcc taacatcacc
    37021 gccgggctgg tcaccgagat cactcgcgca ctgtcgatgg caattctcga tatcgccgcg
    37081 ctgggctttc tcgatctcgg cgcacaactc ccctcgcctg aatggggagc gatgctcggt
    37141 gatgcgctgg aactgattta tgtcgccccg tggactgtca tgctgccagg tgcggcaatt
    37201 atgattagcg tgctgttggt taacctcctt ggcgacggtg tccgtcgtgc aattattgcg
    37261 ggggtggaat aatgccatta ctcgatattc gtaacctgac tattgaattt aaaaccgggg
    37321 atgagtgggt taaagccgtc gaccgggtaa gtatgacctt aaccgaaggt gaaatccgcg
    37381 gtcttgttgg tgaatccggt tccggcaaga gtttgattgc gaaagcgatt tgtggggtga
    37441 ataaagataa ctggcgtgtt actgctgacc gtatgcgttt tgatgatatc gatttgctgc
    37501 gtctctccgc acgcgaacgg cgcaaactgg ttggccacaa cgtgtcgatg attttccagg
    37561 aaccgcagtc gtgtctagat ccttcagaac gtgtgggtcg ccagttgatg caaaacatcc
    37621 cagcctggac ctataaaggc cgttggtggc agcgttttgg ctggcgcaaa cggcgtgcca
    37681 ttgaactgct gcaccgcgtg gggattaaag atcacaaaga tgcgatgcgc agttttccct
    37741 atgagttgac cgaaggtgaa tgtcagaaag tgatgatagc aattgcactg gcgaatcaac
    37801 cgcgtctgct gattgctgac gaaccgacaa actcaatgga gccaacaacc caggcgcaaa
    37861 tctttcgcct gctgacgcgt ctaaaccaaa acagcaatac cactattttg cttatcagcc
    37921 atgacttaca aatgcttagc caatgggcgg ataaaattaa cgtgctttac tgcggtcaaa
    37981 cggtggaaac cgcgccgagt aaggagttgg tgacgatgcc acatcatcct tatacccagg
    38041 cgctgatccg cgcgatacca gacttcggca gcgcgatgcc gcataaaagt cgcctcaata
    38101 cgctacccgg cgctatccca ctgctggaac agttaccgat tggttgtcgt ctggggccac
    38161 gttgcccgta tgcacaacga gaatgcattg tgacgccacg tttgacgggg gcgaaaaatc
    38221 atctctatgc ctgtcatttc ccgctgaaca tggagaaaga gtgagatgat cgaaacgctg
    38281 cttgaagtgc gtaatttaag taaaacgttc cgctaccgga ccggttggtt tcgtcgtcag
    38341 accgtagaag cggtaaaacc cttgagcttt acgctacgtg aaggccagac actggcgatt
    38401 attggcgaga atggttcggg taaatccacg ctggcaaaaa tgctggcggg aatgatagaa
    38461 cctaccagcg gcgagttatt gattgacgat catccactgc attttggcga ttattccttc
    38521 cgcagtcagc gtattcgcat gatttttcag gatccttcga cctcgttgaa tccccgtcaa
    38581 cgtatctcgc aaattctgga ttttccactg cgcctgaaca ccaacctgga accggaacag
    38641 cgtcgcaaac agattattga aacgatgcgt atggttgggc tgctaccgga tcacgtcagt
    38701 tactatccgc atatgttggc ccccgggcaa aaacagcgtc tgggtctggc gcgcgcgctg
    38761 atattgcgcc caaaagtcat tattgccgat gaagccctcg cttcactgga tatgtcgatg
    38821 cgttcgcagt tgattaatct gatgctggaa ttacaggaaa aacagggcat ttcgtatatt
    38881 tatgttaccc agcatatcgg aatgatgaag cacattagcg accaggtgct ggtgatgcat
    38941 cagggagagg ttgtcgagcg aggcagcacc gcggatgtgc tggcatcgcc gctgcatgaa
    39001 ctcaccaaac ggctgattgc cggtcatttt ggcgaggcat tgacagcgga tgcgtggcgt
    39061 aaggaccgtt agtgttctgc acctcatcct gacaggggcc cccctcagga tgaggtattc
    39121 attacttttg attgtcgatt ctggtgagag caaccaggaa tgccagtgta taacacaccg
    39181 taattgccag actcattttt aacagtgttt ctccgccaaa tcccgacaat ggcgcggcta
    39241 taccgccgaa gacaaacatg ctcatcccca tcaacgcaga cgccgttcca gattcctgtg
    39301 tccctactgc actcatagcc tctgccccgc caacagtgcc gatgccgcta caaaatgcaa
    39361 tagtgaaaaa taatgccacc agtgccggaa tgggcaattg tgtccatgcg cataagaccg
    39421 tgagcaatgc acacaaaatt gccgctatca ggccacctcg caacaatgtc atcgcattaa
    39481 tccgtcgcgc cagacgcgaa aagatccatg aagcaatgat aagtccgatg ccgttaaggc
    39541 caaacaccag gctaaattgc attggactaa agccaaattc cttctgcaac acgaacgacg
    39601 aagagccgat atacgcaaac aagcctgcca gcataaaact ttgaatcagg caaaagcgca
    39661 taaaacggcg gttctgcacc acgcttcgtc cggttaatag caatgatgag ccccttttat
    39721 tttctggcaa ggtctcatta ataaataaca cgcagcccag caacaatacg gtgctaattt
    39781 cagccattac ccagaataaa gtgcgccagt caaaagtgct aacaatgtac ccgcccagca
    39841 ctggcgacaa caccggtgcc aggccattca ccgtcattaa cagcgcaaaa aactgggtca
    39901 acgttactcc ctgatatttg tcacgagcaa tagaacgaga gagcaccgaa ccacccgccc
    39961 ccgcgatccc ttgaataaaa cgccagacca ccagccagta aatattgttc gttgtcgcgc
    40021 acaaaatgga agaaacaata aacaatagca acgacaagat taacggacgt ttacgcccta
    40081 ttttgtcgct cagagggcca aataacagtt gtccgacgcc aagaccgatc agtgatgcag
    40141 tcagcgttaa ttgcgttatg gtcgtggttg ccgccagttg ctcagacatc tccggcaaag
    40201 ccggcaaata aagatcggta cacatcgggc cgataccggc taaaagacca agaatcaatg
    40261 cccatgaaag agagactcta gccatattat tccttacttg agtgatgaca tgccgccgcc
    40321 cagcgactgc cagagcgtga ttcggtttgt aaaatcagtc agttgcagtg ccagcagctc
    40381 ctgctgtgcg ctccacaagc tgcgctgcgc cgtcaacacg gtcaggtaat caccaacacc
    40441 cgtctgatag cgacgcaagc caacatcgac cgtttgttgc tcagctttaa catactggcg
    40501 ctgtgcatcc agttgctctt caagcgtggt ccgccgcgcc agtgcgttag cgacatcttt
    40561 aaatgcgctc tgaacggttt tttcataggt ggcaatgagt ccacgctttt gtgcttctgc
    40621 gtaacgaagc tgcgccagat tacttccgcc agtgaacagc ggtaatgtga ccgatggcgt
    40681 aaatgaccag atctgcattc cgtggctgaa caacgacgac aatgcgtcac tgccaacacc
    40741 agcgctggca gtcagcgaaa tcgtcgggaa aaagttagcc cgcgccgcgc caatatcggc
    40801 attggcgctc ttcaaattat gttccgcttc ctggatatcc gggcgacgca gcagaacgtc
    40861 agatgacact cccgcaggaa ccagacttat catctgttcc ggcaggcttt ccagtgttcc
    40921 cggtagcaaa ttttcctcaa gcgttgtacc tgccagtaag ttcagcgcat ttttatcctg
    40981 catcacttgt gtctgatagc tggcaacgct ggcgcgcgct tgctgataaa cactcatcgc
    41041 ctcactgaca tctgtcgctg ccgccgtgcc aacctgttgc tggcgctgga taattttcaa
    41101 tgagttttct gcactggtca tcgtttcttt tgccagggcc aggttgctgt tatcggccgc
    41161 cagtgtcagc caggcggtgc tgatttcagc aatcagcgtt aaacgggtgt tctgggctgt
    41221 aaattcactg gcaagccagg tttctcgagc cgcccgagat aaactctgat tacgaccaaa
    41281 cagatctaac gtaaaactgg acaccgtgcc gtcagcctcg gcagttgttc ccgtaccatt
    41341 cgccagcgaa cgactgcggg tcgaacttaa ggccgcattc accgtcggga ataacgacgc
    41401 attagtttgc ccatagagcg cccgtgctga gtcaatatca gcaatcgctt tttgcacatc
    41461 gcggttacta ttcagtgcaa tagtcaccac ctgctgcaat cggggatcgt ggatcacctg
    41521 ctgccagtca tggctgattg cctttccctg gccctgtgct cccggcaagg tcgccgggat
    41581 tggggattca ggcgtgctgt agtggggatc gagcgagaca catcccgcac tcaacagcac
    41641 cagaaaaata agagtacgac gcaacataac ttactcctga cggcgcgatt taccggcaaa
    41701 caaacgtttc accagtacaa aaaacagagg aacaaagaaa atagccagca acgtagcggt
    41761 cagcgtaccg ccaataatgc ccgtaccaat ggcgatgcgg ctgttcgccc ctgcgccggt
    41821 tgctatcgcc agcggcatta cccccgcaat aaacgccagc gaggtcatga tgattgggcg
    41881 taaacgagtc tgcgccgcgc gtaatgccgc acgactcaag gaatagccct ctgcaaccgc
    41941 cgcttcagca aattccacaa tcagaatggc gtttttcgac gacaagccga tagtggttaa
    42001 tagcgccacc tggaagtaaa catcgttgtt taaatcacgc atccaggccg ccagcgccgc
    42061 gccaagaagc cccagaggga tcaccagaat gaccgagaac gggactgacc agctttcgta
    42121 cagtgctgca aggcagagga aaaccaccag aatagaaact gcatacagac tcaatgcctg
    42181 accgctggcc agtttctcct gcaatgacaa accactccag gcccaggttg ttcccgcagg
    42241 aagactgttt gccagttcct ccatttttgt cattgcatcg ccggaactaa agccagtcgc
    42301 attttccccc tgaatttcat aggctgcaga accgttgtag cgcaccagtc tttccggtcc
    42361 atacagccag cgggtggtcg caaaagcaga gaatggtgtc atggcgttat cgctaccgcg
    42421 cacaaaccat ttacctaagt ctgacggcgc ggaacgaaat tcactgtcgc cctgaatgta
    42481 gacttttttc acacgaccac ggtcaataaa gtcattcaca taagtaccgc cccacgcact
    42541 ggacagggtg tcggtgacat cattcaaact caaccctaat gacaccgctt tattactatc
    42601 aatatctacc tgcaattgcg gcatttgtgg caaatcattg gcacgcacag aatgtaactc
    42661 cggactttga ttcgcttttt ctatcaactg attacgcatt tgcagtagtg tttcgcgatc
    42721 ggttccaccg ttagctaaca attcaaacgt aaaaccattg ctttgcccca gcccatcaac
    42781 ggctggcggc gtcatcgcaa ataccgtggc atcacgaatt gtgcccagct ctttggttgc
    42841 ccgtagggcg atagcctggg cggtgttttc tgccccttta cgttgagacc agtttttcaa
    42901 cgaaacaaac gccatcccgg tgttctgtcc gctgccgcta aaactgaaac catcaacggt
    42961 aaaaatgaca tcggtatttg ctttctcgtt aatcaggaac cagtcaacaa tctggcgatt
    43021 cacttctgct gtacgggcag cggtcgcccc ggccggcagc gtgtactgca ccatgatttc
    43081 gccctggtct tcagtgggta agaaactgcc cggcagtttc cacatcatca gggccattcc
    43141 accacccagt acgagataaa gccccatcgt tcgagcagca cggcgcagga cataaattac
    43201 gcctcgctga tatttatctt cagtacggcg gtaaaagcgg ttaaatgcgc cgaaaaagcc
    43261 ttttttatgt ggcggaacat gctggaggac ggaaccgcac agggcgggag tcaaggtcaa
    43321 tgccaccacc acggaaagca gcattgcgga gataatggtt atcgaaaact gacgataaat
    43381 tacccccgta gatcccccaa agaaggccat aggcaggaat acggctgaca acaccagcgc
    43441 aatggcaacc agtgcgccag aaatctcgcc cattgatttt tccgtggctt cacgcgcggg
    43501 caacccttcg tcacgcataa tgcgttcgac gttctccact accacaatgg catcgtccac
    43561 cagcaagccg atcgccagca ccattgcaaa caacgtcagg gtattaatcg aatagccaaa
    43621 caacgcaaga acgccgaatg tgcccagtaa aacgacgggg acagctaacg ccggaatcag
    43681 tgtggcacgc aggttttgca ggaataaata catcacgcaa accaccaata cgatggcttc
    43741 aaacagcgtc tgaattacgt cctctacaga gattttaata aattcagtgc tgtctttcgg
    43801 gtaagcgatg tcgtatccct gcggcatgtt ccgctggaat tcggcaatct tatccttgac
    43861 cagcgtcgcc gtattcagcg cattcgcacc gggcgaaagc attacagcaa ccccggcaga
    43921 cgggtgaccg ttaagtttgc cgatagcggt ataatcttca ctgcccatct caacgcgagc
    43981 cacatctttt atgcgaacaa ctgcgccgtc tgactggctt ttcacgataa tatttttgaa
    44041 ctgatccacc gtctgcaagc gagactgcgc acgtaccgtt gcggtcagtt gctgagtatt
    44101 cggtgaaggc aatgccccta ttttcccggc agtaacctgc acgttttgcg cttcaatagc
    44161 actttgcacg tctgaaggca ttagcgagta agacgccagt ttggccgggt caagccagat
    44221 gcgcatagcg tattccgcgc caaagacttg cagactcccc acaccttcaa cacgcgccag
    44281 cgggtcctga acgttactaa ccaaccagtc ggcgatatcc gaactggagg ctttgtcagt
    44341 ggtgtcatac acggcagcaa tcagcaaaaa gttgctttgt gatttctcca ccgtaacacc
    44401 cgtttgctgc acttcgctgg gtaggcgcga ctccgcctgc tgaattttat tctgcacctg
    44461 aacctgtgca gtatctggat cggtaccttg ttcaaaggtc acattaatac tgaccgaacc
    44521 atcagagctg ctggttgagc tgaaatagag taaattatca agcccagtga gttgttgctc
    44581 gataacctga gtcacgctgt tctccagcgt ttcggcagaa gcaccagtat aagtggctga
    44641 aattttaatg gtcggtggcg caacgtcagg atattgcgcg acgggcaatg tgcgaatagc
    44701 cagaatcccc gccaacataa tcaaaatggc gataacccag gcaaagaccg ggcggcgtac
    44761 aaaaaagcgc gaaaacatca ggcgttgcct ccattagcct gaacttcaac agccttgacc
    44821 gtctgccctg aggtgacttt tgcagaacct tcaacaatca gtcggtcgcc gttgtgcagg
    44881 ccgtttagca ccagccattt atcaccatat gtttctcccg tttcgagcgt tcgctgctct
    44941 actttattgt ctttattcac caccagcgca gttgcattgc ctttagcatc gcgcgtgacg
    45001 ccctgttgcg gcgcgagaat agcgtcttcc atcacgcctt catctacacg agcgcgaaca
    45061 aacattcccg gtaataactg ctgttgtgga ttggggaaaa tcgcccgtaa tgtcaccgaa
    45121 ccggtagact catctacagc gacttcggtg agttccagac gccctttttc gctgtaggtt
    45181 gtgccatctt ccagaataag tgagacgctc atggtgtcac tgttggtcgc cagtgactgt
    45241 ttacgcagac gtattaaatc gacactggag cgagtgaggt cgacatacat tgtatccaga
    45301 ccacgaatag tcgttaacgc tgtatcttgc gacgcggtca ccagtgcgcc aggggttacc
    45361 gacgaaatgc caatgcgccc tgaaatcggt gcggttaccg tggtccagtc aagattaatg
    45421 cgcgcagttt ccagtgcggc ttttttcgcc tctacactgg ctttatcttg tgcacaggta
    45481 gactgagcat catcagcatc ctgttgtgaa acaccgttct ctttcaccag tcgggcataa
    45541 cgctgcgctt tctggcaatc ggcttttacc agtgcctgtg cttgttgtaa tgctgcccga
    45601 gcttcattcc atgcagcctg ataactggcc gcatcaatct ggtagagcgg ctgtccagcc
    45661 ttgaccagat caccttcctt aaataagcgt ttctggataa ttcccccaac ctgcggacgt
    45721 acttcggcac tgagcgcagc actggtgcgt ccggttaatt cactgactac cgagaccggt
    45781 tgagatttga ctgtaacaac gccgacttct gtttctgacg gggatgaatt gttactttgc
    45841 gtattatcac aaccagataa gagcagcatt gccactacag atgttgctat atacttcatt
    45901 attttctcca gggtgagaaa taccctcctc attaaaatta atgaggatat gtcagggata
    45961 ttcagttata acgaacccgc atcgcgaaat gaatttcgca atgaaataag ggggattaat
    46021 atcccccgct attggttaat tctgttctta tttatttatc aacatactaa caatatcctg
    46081 atatatttcc tgcagatgtt gtggatcgct tttttgcggt gttaaacgac gataaaccgt
    46141 accttccatc atggtagcaa acacttcaac gcagcaacga atgtgctcgt cgctaagatg
    46201 gggatgcatt cttttcatat gtgcacaacc gttagcgaac attctggcgt cagcttcttc
    46261 aagcattgcc ataacccgcg ggttgcgtgt ggcttctgca gccacttcca gcatcagcgc
    46321 gtcgtcgtcc tcatttaagg atttacgcaa ggcaaggact tccgggaagt gatcggtacg
    46381 cgcgtcaatg tccatttggg caatacgaaa gtcgatgata cggcgaacca tctcttcaat
    46441 aatggcgtct ttgttggcga agtaacgata tatctgaccc acgctaagct gagcttcgct
    46501 ggcaatttgc gacatactgg cggcatgaaa cccgcagcga cgaaaacacc gacgggcggc
    46561 gttaatgatt tcatcctgac gctgtttgtg gcgtatctcc agcttgctgg tcatttctgt
    46621 ctcctggcct atcacttggt gagaatgatc gttctcattt ttgctgatta tacgtttctc
    46681 caaagtaatg gaaagtattt ttatcttatt catggtgcat attctgataa tgaagagatt
    46741 gtggagattc tgtgaagata gttgacaata ttaagttggg gtcaattcat cagaaaggct
    46801 gctacttgtt gtcgccagtt ctgttttcat catcaaactt atcggagcta atacgaatag
    46861 tttcatagtc agattctata agatatttcc ccaacctacc ccttgcaggc gtcaagagta
    46921 gataacgcaa aatgctctgt accgcagctt ctctccggta cagaaagcgc aactatagcc
    46981 acccacagcc aggttgatta taataaccgt ttatcagttc gtactgttta ctaaaacgac
    47041 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    47101 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    47161 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    47221 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ccaatatcgt tctgcaatgc
    47281 gatgttgccg aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    47341 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    47401 tatgttaacg ccgttactcg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    47461 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    47521 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    47581 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    47641 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    47701 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
    47761 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    47821 gtggtccacg ttgacggcgg tttcagcatt gctgcaatga acgaactcga actgaaataa
    47881 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    47941 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    48001 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    48061 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    48121 caaaggtcag cgatcccttt ctactcgacg tgatccttga aaaagaaacg ctggccccct
    48181 tcctttcatg gctggaccct gcgcgtgttc ttgcagtgga tttgttccct gaccagctta
    48241 ccgttacccg ttcacagacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    48301 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    48361 ccgggcctga ttccaccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    48421 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    48481 tcggtcttac tgcctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    48541 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    48601 ggctggagat acaaaactac gccgacgcgt cactgtttgc tgatgaatca ttacgctggc
    48661 gaagcagcta tgaggtggaa aaactacctg aagaacacac aaaaagttca actgcagaac
    48721 tttgccaact ggccgaaata ctctctctcc accctatttc actttccctt ctcgaaaggt
    48781 tgttaaacag cacacgggtt aatacacagc cggataatca gcttgcggcg ttgttatgcg
    48841 cgcggataaa tggcagtcct gcttgttttg ccgcctgtat ggattcatca aatgaatata
    48901 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccaatgg gctgaccgtc
    48961 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    49021 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    49081 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    49141 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    49201 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    49261 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    49321 ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    49381 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    49441 ccagaggaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    49501 gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc ttgccgcgca
    49561 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    49621 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    49681 gacgaccact ttgtaccgtg gtgggttaca cttgcgcgcc ataatctgga aaaagaagca
    49741 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgaccgcg
    49801 ctggattttg tcaccatcga cagtgcaagc acagaagata tggatgacgc ccttttcgct
    49861 aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    49921 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctt
    49981 cctggcttca acatccctat gcttcctcgc gagctttctg acgatctctg ctcactgcgc
    50041 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    50101 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    50161 caggtttctg actggctgga gaataccggt gactggcagc ctgaaagtga agcaattgcc
    50221 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    50281 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    50341 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    50401 gccgctaaca tttgtgcggc tcgcgtactg cgcgataagc tcggttttgg tatctataac
    50461 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    50521 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    50581 gaactggatg cgcaaccaac tggtttcctc gacagtcgca ttcgtcgctt ccagtcattt
    50641 gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    50701 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    50761 atcaaaggcg aaactgcgac gcgtccacag gatgaaatca ctgtgcaaat ggccgagcgt
    50821 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    50881 aaagacaaag ccgggaccga cacccgtttc tcggcggaaa ttgtcgatat cagccgtggc
    50941 ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    51001 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    51061 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    51121 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    51181 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    51241 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaaaca
    51301 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    51361 ctccgcagtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    51421 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgttttgcg
    51481 cttttctacc accgaaacca cagaacctga acgtacgttg cagttatctg ccgaacaggc
    51541 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    51601 tgagagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    51661 caacgcgtct gcatggcatg acacaccagc agttgctcgt gatttatcac acggactttc
    51721 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    51781 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaacg aacacgacgt
    51841 cattggccaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    51901 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    51961 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    52021 aaacgagatt tttttaatct gttccggcac cgacattacc gaagagcgcc gcgctcagga
    52081 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    52141 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggtcgtgta
    52201 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    52261 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    52321 gcgtccaggt ggcgatgagt ttctggtact ggcatccaac acctcacaaa gcgcgctgga
    52381 agcaatggca tcacgaattt tgacccgctt acggctcccc tttcgcattg gtttaattga
    52441 agtttatacc agctgttcag taggtattgc actctctccc gaacatggtt cggacagcgc
    52501 ggctattatt cgtcacgccg acacagcaat gtacacagca aaggaaggcg gacgaggaca
    52561 attttgcgtt tttaccccag aaatgaatca acgggtattt gaatatctct ggctggatac
    52621 caacttgcgt aaagcactgg aaaacgatca gttggttatt cactatcaac cgaaaatcac
    52681 ctggcgtggc gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg
    52741 gttgattcca ccgttggact tcatttccta cgcagaagag tcagggctaa ttgtgccttt
    52801 aggccgttgg gtgattctcg atgtcgtacg ccaggtggcg aagtggcggg ataaaggcat
    52861 taacctgcga gtggcggtaa acatttctgc acgtcagctc gccgatcaaa ccattttcac
    52921 cgccctgaaa caggttctcc aggaactcaa ttttgaatac tgccctattg atgttgaatt
    52981 gacagagagt tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag
    53041 ccaactaggt gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca
    53101 actggcgcgc tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca
    53161 caaacaacct gtctcgcagt cactggttcg ggcaatcgtc gctgtggccc aggcattgaa
    53221 tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa
    53281 tgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga
    53341 acgctggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct
    53401 atattccgtc attatatatc gtattcccgg atgataatta gccgttgctg ttaaacactt
    53461 ctcttttagc ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt
    53521 gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct
    53581 tgtggcaggc
//
LOCUS       DAPGRN010000017        53439 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPGRN010000017 DAPGRN010000000
VERSION     DAPGRN010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.017"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..1761
                     /locus_tag="RHW33_001143"
     CDS             31..1761
                     /locus_tag="RHW33_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV1644668.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAAQSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFSCQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPDDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
     gene            1758..2672
                     /locus_tag="RHW33_001144"
     CDS             1758..2672
                     /locus_tag="RHW33_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV1644669.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKYVAEQRLTAMADKITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVADGYDW
                     QRVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            2704..2952
                     /locus_tag="RHW33_001145"
     CDS             2704..2952
                     /locus_tag="RHW33_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV1644670.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFNISIPINILLDVKTPAQLMETLLPWLENK"
     gene            2952..4124
                     /locus_tag="RHW33_001146"
     CDS             2952..4124
                     /locus_tag="RHW33_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV1644671.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHMALEKEIAAFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="RHW33_001147"
     CDS             complement(4159..5238)
                     /gene="lptG"
                     /locus_tag="RHW33_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV1644672.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMDLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPIAFVLAALILVYLFDRQH"
     gene            complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="RHW33_001148"
     CDS             complement(5235..6305)
                     /gene="lptF"
                     /locus_tag="RHW33_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV1644673.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFTLLQKFSR"
     gene            complement(6336..6896)
                     /locus_tag="RHW33_001149"
     CDS             complement(6336..6896)
                     /locus_tag="RHW33_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV1644674.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLPEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(6908..7732)
                     /locus_tag="RHW33_001150"
     CDS             complement(6908..7732)
                     /locus_tag="RHW33_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644675.1"
                     /translation="MMRKYFPLEASEHLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            complement(7732..9081)
                     /locus_tag="RHW33_001151"
     CDS             complement(7732..9081)
                     /locus_tag="RHW33_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV1644676.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAAHELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(9127..9885)
                     /locus_tag="RHW33_001152"
     CDS             complement(9127..9885)
                     /locus_tag="RHW33_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644677.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(9917..10630)
                     /locus_tag="RHW33_001153"
     CDS             complement(9917..10630)
                     /locus_tag="RHW33_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV1644678.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCRSQQGHLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            10804..11496
                     /locus_tag="RHW33_001154"
     CDS             10804..11496
                     /locus_tag="RHW33_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV1644679.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(11544..13043)
                     /gene="pitB"
                     /locus_tag="RHW33_001155"
     CDS             complement(11544..13043)
                     /gene="pitB"
                     /locus_tag="RHW33_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV1644680.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(13335..15194)
                     /gene="gss"
                     /locus_tag="RHW33_001156"
     CDS             complement(13335..15194)
                     /gene="gss"
                     /locus_tag="RHW33_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV1644681.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            15399..16265
                     /gene="yghU"
                     /locus_tag="RHW33_001157"
     CDS             15399..16265
                     /gene="yghU"
                     /locus_tag="RHW33_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV1644682.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGAEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(16388..16636)
                     /gene="hybG"
                     /locus_tag="RHW33_001158"
     CDS             complement(16388..16636)
                     /gene="hybG"
                     /locus_tag="RHW33_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV1644683.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(16649..16990)
                     /gene="hypA"
                     /locus_tag="RHW33_001159"
     CDS             complement(16649..16990)
                     /gene="hypA"
                     /locus_tag="RHW33_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV1644684.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCQGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(16983..17471)
                     /gene="hybE"
                     /locus_tag="RHW33_001160"
     CDS             complement(16983..17471)
                     /gene="hybE"
                     /locus_tag="RHW33_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV1644685.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSIPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(17464..17958)
                     /locus_tag="RHW33_001161"
     CDS             complement(17464..17958)
                     /locus_tag="RHW33_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV1644686.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(17958..19661)
                     /gene="hybC"
                     /locus_tag="RHW33_001162"
     CDS             complement(17958..19661)
                     /gene="hybC"
                     /locus_tag="RHW33_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV1644687.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(19658..20836)
                     /gene="hybB"
                     /locus_tag="RHW33_001163"
     CDS             complement(19658..20836)
                     /gene="hybB"
                     /locus_tag="RHW33_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV1644688.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIGKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(20826..21812)
                     /gene="hybA"
                     /locus_tag="RHW33_001164"
     CDS             complement(20826..21812)
                     /gene="hybA"
                     /locus_tag="RHW33_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV1644689.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(21815..22933)
                     /gene="hybO"
                     /locus_tag="RHW33_001165"
     CDS             complement(21815..22933)
                     /gene="hybO"
                     /locus_tag="RHW33_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV1644690.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(23122..23409)
                     /locus_tag="RHW33_001166"
     CDS             complement(23122..23409)
                     /locus_tag="RHW33_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV1644691.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(23528..24415)
                     /locus_tag="RHW33_001167"
     CDS             complement(23528..24415)
                     /locus_tag="RHW33_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV1644692.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWLAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            24572..25612
                     /gene="gpr"
                     /locus_tag="RHW33_001168"
     CDS             24572..25612
                     /gene="gpr"
                     /locus_tag="RHW33_001168"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV1644693.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMELEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPEDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALSNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(25652..26146)
                     /locus_tag="RHW33_001169"
     CDS             complement(25652..26146)
                     /locus_tag="RHW33_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV1644694.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            26337..27221
                     /gene="yghA"
                     /locus_tag="RHW33_001170"
     CDS             26337..27221
                     /gene="yghA"
                     /locus_tag="RHW33_001170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV1644695.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEALPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(27493..27918)
                     /gene="exbD"
                     /locus_tag="RHW33_001171"
     CDS             complement(27493..27918)
                     /gene="exbD"
                     /locus_tag="RHW33_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV1644696.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(27925..28659)
                     /gene="exbB"
                     /locus_tag="RHW33_001172"
     CDS             complement(27925..28659)
                     /gene="exbB"
                     /locus_tag="RHW33_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV1644697.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            28911..30098
                     /gene="metC"
                     /locus_tag="RHW33_001173"
     CDS             28911..30098
                     /gene="metC"
                     /locus_tag="RHW33_001173"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV1644698.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIATIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFS
                     RIV"
     gene            30238..30897
                     /locus_tag="RHW33_001174"
     CDS             30238..30897
                     /locus_tag="RHW33_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1644699.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(30937..31836)
                     /gene="yqhC"
                     /locus_tag="RHW33_001175"
     CDS             complement(30937..31836)
                     /gene="yqhC"
                     /locus_tag="RHW33_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV1644700.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGMRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            32030..33193
                     /gene="yqhD"
                     /locus_tag="RHW33_001176"
     CDS             32030..33193
                     /gene="yqhD"
                     /locus_tag="RHW33_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV1644701.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            33298..34125
                     /gene="dkgA"
                     /locus_tag="RHW33_001177"
     CDS             33298..34125
                     /gene="dkgA"
                     /locus_tag="RHW33_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV1644702.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            34325..35251
                     /locus_tag="RHW33_001178"
     CDS             34325..35251
                     /locus_tag="RHW33_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV1644703.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITETD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKAANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTVDSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            35300..35557
                     /gene="yqhH"
                     /locus_tag="RHW33_001179"
     CDS             35300..35557
                     /gene="yqhH"
                     /locus_tag="RHW33_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV1644704.1"
                     /translation="MKTIFTVGAVVLATCVLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(35600..37819)
                     /locus_tag="RHW33_001180"
     CDS             complement(35600..37819)
                     /locus_tag="RHW33_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV1644705.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(37930..39342)
                     /gene="ftsP"
                     /locus_tag="RHW33_001181"
     CDS             complement(37930..39342)
                     /gene="ftsP"
                     /locus_tag="RHW33_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV1644706.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(39417..40154)
                     /gene="plsC"
                     /locus_tag="RHW33_001182"
                     /gene_synonym="parF"
     CDS             complement(39417..40154)
                     /gene="plsC"
                     /locus_tag="RHW33_001182"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV1644707.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(40388..42646)
                     /gene="parC"
                     /locus_tag="RHW33_001183"
     CDS             complement(40388..42646)
                     /gene="parC"
                     /locus_tag="RHW33_001183"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV1644708.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMLRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(42784..44391)
                     /locus_tag="RHW33_001184"
     CDS             complement(42784..44391)
                     /locus_tag="RHW33_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1644709.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPTNTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGRRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLCFELFYNKYDLHEKI
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(44500..44982)
                     /locus_tag="RHW33_001185"
     CDS             complement(44500..44982)
                     /locus_tag="RHW33_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDV1644710.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELIGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(45035..45427)
                     /gene="ygiW"
                     /locus_tag="RHW33_001186"
     CDS             complement(45035..45427)
                     /gene="ygiW"
                     /locus_tag="RHW33_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV1644711.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVENAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            45579..46238
                     /gene="qseB"
                     /locus_tag="RHW33_001187"
     CDS             45579..46238
                     /gene="qseB"
                     /locus_tag="RHW33_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV1644712.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGCQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLILKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            46235..47584
                     /gene="qseC"
                     /locus_tag="RHW33_001188"
     CDS             46235..47584
                     /gene="qseC"
                     /locus_tag="RHW33_001188"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV1644713.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFVRTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSEDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNADQGGFEAKVSW"
     gene            47694..48275
                     /gene="mdaB"
                     /locus_tag="RHW33_001189"
     CDS             47694..48275
                     /gene="mdaB"
                     /locus_tag="RHW33_001189"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV1644714.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            48306..48620
                     /locus_tag="RHW33_001190"
     CDS             48306..48620
                     /locus_tag="RHW33_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV1644715.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(48665..49552)
                     /locus_tag="RHW33_001191"
     CDS             complement(48665..49552)
                     /locus_tag="RHW33_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV1644716.1"
                     /translation="MIQKDKTRVDIYGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(49549..50496)
                     /locus_tag="RHW33_001192"
     CDS             complement(49549..50496)
                     /locus_tag="RHW33_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1644717.1"
                     /translation="MRLFFSLLILLSFFARATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLTACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(50507..51544)
                     /locus_tag="RHW33_001193"
     CDS             complement(50507..51544)
                     /locus_tag="RHW33_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1644718.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCAREISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(51553..52536)
                     /locus_tag="RHW33_001194"
     CDS             complement(51553..52536)
                     /locus_tag="RHW33_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1644719.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFEP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGLTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGVVLAIYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(52533..53342)
                     /locus_tag="RHW33_001195"
     CDS             complement(52533..53342)
                     /locus_tag="RHW33_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1644720.1"
                     /translation="MAHNAKGQGLILDNVSARYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT        13382 a        14252 c        13339 g        12466 t
ORIGIN      
        1 gtcattttat tttggatatc caattttttc gtggtgtata tgtctaataa aatctttacg
       61 cattccctac ctatgcgcta tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc
      121 gctcagagta gcgctggaat gaatttttat gacagacgtt gccaacttga agatcaactg
      181 gaatatcaga cgttaaaagc gcgtgccgaa gctggtgcga agcggttgtt atcgctaaac
      241 ctgaaaaaag gcgatcgcgt ggcgctgatt gccgaaacca gtagcgggtt cgtagaggct
      301 tttttttcct gccagtatgc cggcttagtc gccgtcccgt tggcgattcc aatgggcgtc
      361 ggtcagcggg attcctggag cgccaaactg cagggtttac tggcaagttg ccagcccgct
      421 gccattatca ctggtgatga gtggttgccg ctggtcaatg ccgccacgca taacaacccc
      481 gaattgcatg tattaagcca cgcctggttt aaggcattac cggaagctga tgttgcgctc
      541 cagcgcccag ttccggacga tatcgcctac ctccagtaca cctccggcag cacccgtttt
      601 ccccgtggcg tcattatcac ccatcgcgaa gtgatggcta atctacgtgc tataagccac
      661 gatggcatta aattacgccc tggcgaccgc tgcgtctcct ggctgccttt ctaccatgat
      721 atgggactgg tcggctttct cctgaccccg gtcgccacgc agctttcagt agattatttg
      781 cgcactcagg attttgccat gcgtcctctg caatggctta aattgatcag taaaaatcgc
      841 ggcaccgttt ccgttgcgcc gccgtttggc tatgaattgt gccagcgccg cgtgaatgaa
      901 aaagatctcg ctgaactgga tctttcctgc tggcgcgtcg ctggtattgg tgcagaaccc
      961 atctccgcag aacaactcca tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat
     1021 aaaactttca tgccgtgcta cggcctggca gaaaatgcgc tggctgtcag cttctctgat
     1081 gaagcttccg gggttgtggt taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa
     1141 gccgtcgcgc cgggtgcaga gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg
     1201 ttgccggaac atggtattga aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg
     1261 gtaggccata tttgcatctc cggccccagt ctgatgagcg gttactttgg cgaccaggct
     1321 tcgcaagacg agattgccgc gacgggctgg ttagacaccg gcgacctcgg ttatctgctg
     1381 gacggttatc tgtatgtcac cggacgcatt aaagatctga ttattattcg tggccgtaat
     1441 atctggccgc aggatattga atatatagcg gaacaggaac cggaaattca ttctggcgat
     1501 gcgattgctt ttgttaccgc ccaggaaaaa atcattttgc agatccagtg tcggatcagc
     1561 gacgaagaac gtcgcgggca gcttatccac gcgctggcag cacggatcca aagcgaattt
     1621 ggcgtgaccg cggctatcga tctgttgccg ccccacagta ttccccgaac gtcctccgga
     1681 aagcctgccc gtgcggaagc gaaaaaacgt tatcagaagg cttatgctgc aagccttcat
     1741 gtgcaggaat ccctggcatg aatcaaactg tcgcggtgac gggcgctacc gggtttatcg
     1801 gtaaatatat tatcgataac ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta
     1861 ctgcccgcgc tcacgtcaat gacaatctca cctgggtgcg cggttcgctg gaagatacac
     1921 attcgcttag cgaactggtt gccggagcca gcgcggtggt tcattgcgcc ggacaagtgc
     1981 gcgggcacaa agaagagatt ttcacccgct gtaacgttga cggcagtctg cgcctgatgc
     2041 aggcggcaaa agagagcggt ttttgccaac gttttctgtt tatctcttcg ctggcggcgc
     2101 gccatcccga actctcctgg tacgcaaatt ccaaatacgt cgccgaacaa cggctgacgg
     2161 caatggctga caaaattacg ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg
     2221 ataaagagtt aaaaccgctg tttgactgga tgctgcgcgg cctgctgcca cgacttggtg
     2281 cgccagatac acaactctct ttcctgcacg tcaccgattt cgcgcaagca gtgggtcagt
     2341 ggttaagcgc cgaaactgta cagacgcaaa cctatgaatt atgcgatggc gtcgccgacg
     2401 gctatgactg gcaacgcgta cgacagcttg ccgccgacgc ccgctgtggt tccgtgcgaa
     2461 tggttggtat tcctctgccg gtcctcacct gccttgcgga tattagtacc gcgttgagcc
     2521 gcctggcagg taaagaacct atgctgaccc gctcgaaaat tcgtgaatta acccacgccg
     2581 actggtcggc aagtaataac cgtatttctg aagatattaa ttggtttccc gggattagcc
     2641 tggaacacgc attacgcaac gggctatttt gattgtcttg tttaaaggtg tgttaacgtt
     2701 tctatggtaa atcgtgaaat agtaatggat tacatccttt cctgtttaca ggatttggtc
     2761 gaaaacggtg tggaaattaa gcccgacagc gatctcgtca acgatcttgg cctggagtcg
     2821 atcaaagtga tggatctctt aatgatgctg gaagacagat tcaatatttc cattcctatt
     2881 aatattctgc tggatgtcaa aacccctgcg caattaatgg aaactctact tccatggctg
     2941 gagaataaat aatggggcta tacgataaat atgcgcgcct tgctggcgag cgactgcaat
     3001 ttagtgacaa tgggttaaca ccgtttggca cctgtattga tgaagtgtat tccgccaccg
     3061 aaggccggat cggcaataaa aaagtgattc tggccgggac caataattat ctcggattaa
     3121 ccttcaatca tgatgccatc gccgaaggtc aggcagcgtt agcggcccaa ggcaccggca
     3181 ccaccggttc tcgtatggca aacggcagtt atgcgccgca catggcgtta gagaaagaga
     3241 ttgccgcgtt tttcaatcgc cctaccgcta tcgttttttc aaccggttat accgctaatt
     3301 taggcgtcat cagcgcactt gccgaccata acgccgtggt gttactggat gccgacagcc
     3361 acgccagtat ttatgacgcc tgttctctcg gtggcgcgga aattattcgt ttccgccata
     3421 acgatgctaa agatctggaa cggcgcatgg ttcgcctcgg tgaacgggcg aaagaggcga
     3481 ttatcatcgt tgagggcatc tacagcatgc tcggtgacgt tgcgccgctg gcggaaattg
     3541 ttgatatcaa acgtcgtctc ggtggttatt tgattgtcga tgaagcgcat tcgtttggcg
     3601 tgttaggcgc aacggggcgc ggactggctg aagctgtagg cgttgaagat gatgtggata
     3661 ttatcgttgg caccttcagt aaaagtctgg cctcgattgg cggttttgct gtcggttcgg
     3721 aagcgatgga agtgctgcgc tacggcagcc gcccgtatat ttttaccgcc tctccttctc
     3781 cgtcctgcat tgcgaccgtg cgctcgtcgt taagaactat tgccacacaa ccagagctgc
     3841 ggcaaaagct gatggataac gccaaccatc tgtatgacgg tctgcaaaaa ctgggttatg
     3901 agttaagttc acatattagc ccggtagtgc cggtaataat cggttcgaaa gaggaaggtt
     3961 tacgcatctg gcgcgagctt atttcgctgg gcgtttatgt gaatcttatc ctgccacctg
     4021 ccgcgcctgc aggcatcacg ctgctgcgct gtagcgtaaa cgctgcccac agtcgtgagc
     4081 aaatcgatgc catcattcag gcatttgcta ctctgaagca gtaatgtgag cccgccgtgg
     4141 tgttgcgcca cggcgggttt aatgttgcct atcaaataga tacaccagaa tcaacgccgc
     4201 cagcacaaat gctatcggta aaccgagcgc ggtaacaggt gcgcttaacg caaatagcaa
     4261 tcccagattg accatgattt gatagctgat ccaggtgagt aagccaacga tgacgcctac
     4321 agcaagacgg ctgcccatcc ccggcgagcg cggggcgcta aaggtaaacg gcacagccag
     4381 caaaatcatc gccagggtca ggatgggttg ccccagtttt tcccacagtg caaggcggta
     4441 ttcgctgctg ggttgcccgg tttcctgcaa ataatggatg taatgattaa gctgacggac
     4501 agaaaaagta ttgcccggca ttgataactc ttcaagatcc atactggtga aggccgattg
     4561 ccaggcaaga ttatctaatg tttccagcgt ttctttgcca ttcagccatt ttttatgatt
     4621 cacaccatgc aggatccacg ttttgtcgtc ttcaatggtg gcactgcgtg cataaatgta
     4681 ggattcgagc gagagatcgt cgcgataatg aaatatctcc acgcccacag gctggccctg
     4741 ttcattcagc gatttcaccg tcacaaattc attgccgcgc ctggcccaca gcatattgcc
     4801 ggtaatatca tcgtcctccc ccaacgcagt ggcggtcgat ttgatttgca gcgcctgttg
     4861 ctgcaatggc gacgctaccc actcatctat cgcgcctaac gaaacagtcc acaatatccc
     4921 ggcaaccagc gccaccagtg caatacggaa gatagaaaac cccgtgctgc gaatggcggt
     4981 aagctcactg tttttcgata actgccccag gccaacaatc cctcccaata aggcgataaa
     5041 cggcgaaagt tcgaccagtg tgcgcggtaa ggtcattagc accaccagca ccgcctgagt
     5101 ccagcgataa ccgcccgggc tgacatcatc cagttcgtta atcaggttga aggtggtaaa
     5161 aagcggcagc aatagccctg cggcggcggc aaaaccgaga aagagatgac ggattaaata
     5221 gcgactgaaa acattcatcg ggaaaatttc tgcagcaaag tgaagtcgcg tgcaaccagc
     5281 atcagcaggc ccattagcat gagtccgggc actaaccaca aaccaggaat gagaggaatc
     5341 gcgccgttag ccaccagcgt acggcagacg tcgccgccat aaaaaatggc aataaacagc
     5401 aacgtcagtg gcaataacgt tgaaaatcgc ccctgccgcg gttttacccg acttaatgaa
     5461 atggccagca gcgccatcaa caatgcggtc aggccacggc tttgtcgcca ttgcagttcg
     5521 gcatgatcgg ctggaaagac ggagcgcgcc agctccgtga ccgatttcgc tttgcgtttg
     5581 acgttagggc tttgatccag cggattcaga tgtaattgca gattacggta gatctgttcg
     5641 ttgtcgtcac gcccctgatg atcgagaaga taggcagtgc cgttgtgcaa cataacggtc
     5701 ggtttctcgg gtgatgggtc aaccacatca accgaacggg cgcggaaaat gcgggttcga
     5761 ttggcagtag aagtataaat cagcgcgtca gtcaggcgat tattatcctg atcaaccgtc
     5821 tgcgaaagga tcattcgtcc gttatcgtta gtgttaaatt tctttgcccg caactggcga
     5881 acatccagct ccgactgcga ctgctgctcc agttgataaa tctgcgcata agcccacgga
     5941 cgaccataca tcgacagaag ggtgacaaaa atacttagcg gtatcgccag ataaagaacg
     6001 gctttgtaca agcgtcccgg actgcccccc gccgcagaga tagcggtaat ttccgagtcg
     6061 gtatacatct gccctagcgt cacgccaact gacacataca gaccaacagg taacaacatc
     6121 tccagtgcaa tcagcacttt gtagaaaacg atatccagca caacatcaag agctaatgtc
     6181 ccatttgccg cttcggtcag ataacgctgt gcggagtagc tggcgaaaat aaagatcaga
     6241 aaaccgacga taatcagcac cagacggcgc gtaccacgca tgatgtagtg ttcaaccagc
     6301 ttcatctcac taacgccctc aatcctttga attttttatt cattgatcag tttctgcgcc
     6361 agcgcaatga catgatctac ctcttccggc gtcaggctgc cgtcctttgc ccactgaatt
     6421 tgcccggttt tattcagcac cagaatggtt gaactctgct ccggcaagtg ccaggcaacg
     6481 cgtccctgtc cattgccgtc aataacgaat tgcgcccatg gatagcggcg tttatttttt
     6541 tcgattttgc caacgacaaa atagccggta ccaaagatgg catcatcagt gttcacaatg
     6601 gtggtgggct gaaaacgatc ctgcggaaag tttgccgctt caaccgcttt aattaacagt
     6661 gagttctttt tcttcgccga cttacgcccg gcgatatact gaataatgcg aacttttccc
     6721 gccaattgtg tgctgttcca cttttggtag tgaatttcgt cgtcgctatt cagaagcagc
     6781 tcgccgcctt cctgaatata aacaggggta accgttttgc cagtgacgaa attgtgtgcc
     6841 tgcacacccg gggctaagag catgataatc agcgcaataa tcaaacgaga tgacatcatt
     6901 gctccggtta tagcggaaaa cgcgaaatca gcagctctaa gcgctggcga atatcgctat
     6961 ccagcgccac cggtgcatca tacgactggc ggttttccat atcatccgcc accatgtcgt
     7021 cgtgtttatc ccccatcagg ccattaatgg cggcgaggaa acgggaaatc tgtaacgcct
     7081 ctttattttc aggatcaagc gatcttaagg tgtcgtaata agaaacctgc tggctgatga
     7141 tttttcgcac atcatgaaat tccctgccgg ttagtcgcgg tttggcaata atctctttca
     7201 gggtatgcat ttgcttttgc cgataggcaa taaaaccgct tacgctttct aactggctat
     7261 ggcgtaatga atagttcacc aacgaccaga ccggagagct taaataaatc cgtaacaggt
     7321 tgccgtaata cgacacaata tttttcttac cgttacgaaa cccgtcctga aaacgtccca
     7381 gaaaaacggt cgttttgccg aataaaaatc cggcctgatg cttctttaaa tacagccgtt
     7441 gcgcaaaacg caggtgcttg tagcgcgctc gcatgtattt aaactgttta cgctcgtctg
     7501 tcgtcagctc atcgcctttg gcttgtttgc gaattaaacg aagtaattcc tgacgatcaa
     7561 cgccattgag ccagaattgc aggcataacg cgtaattatc atggatgatt tcggtggtac
     7621 aacttagtgc gatagtcggc ggcaaactga cctgggcatc aacaacatca tcttcctcaa
     7681 tcgcaacaaa tagatgctcg ctcgcctcaa gcgggaaata ttttctcatc atcaggattt
     7741 ctctccggct gcgttataaa gcaaggaatg gcgctgaaaa aatgccttta aggtcggaat
     7801 tagcgaaagc accacatcca gacattgacc gccgaaataa gcaaacgctg ctcccagcac
     7861 gccaaacaga tgacagaaca tgaacagcaa tacgatgtag ccgattgagg cgatggtttg
     7921 cgccacgaga aaagcgcgct gttttcccgc catcaatagc aatgactcct gagggaagcc
     7981 cagcatcgag atcacaattg cgcccagcat cacctgaatc aggtcatacg cttcgagata
     8041 cttaacgcca aacaccagcg aaatgagcgg cttgccgacg atcaacaccg cgagcgccac
     8101 cagaataccg attccgcccg ctagtaaacc ggacttcaca cctaacaacc acggtctggt
     8161 ggtgcgcgga tctaaacgca tcacctctgg gtaaaaactt ttacccagca aacccgctgg
     8221 cgttccggcg gcgtcgaaga atgtcatggc gattttaaat aacccggcgg cagcgggtcc
     8281 taacacgatc cccaccaaca ctgtgctgca tgagttacgc gccgaccaga tggagtgggc
     8341 aatgttggtt gaccagacaa aactccacgc gcctttaatg tgtcgggcag actcaaacag
     8401 attcaatttg aaggcgttat ggatatttct gcggcgtaat tcgtgcgcgg caaaccacca
     8461 gtacatggtg ccgccaacca gattcgacac gtaccaggca ataacaaaac ccgcaaaacc
     8521 aaagtcaaaa taccaggctg cgacgctccc cgctgcgcgc agaaaaggtt tcgtcgcctg
     8581 ctgtacagca atcaaatcaa agcgatctac cgcacgcaga atgccggtcg gcgtggagga
     8641 agccattgaa ggaatgagcg tgcaatacag cgccgccagc caaaagcttt ggtcatccag
     8701 acctaatgaa tgggaaagga atggcagtaa ggcaatgccg ccgacaatcg ccaccgcgcc
     8761 gctgacgata tccagcgaga aggaaaatga gacgacattg cgaaattgct gcggattatt
     8821 gttggttaat gctggtgttc cgtactgaac caccagctgc catgtctgaa acttaataaa
     8881 atcgctgatc gacttggcgt acgattgtac aatcaccagt acgccaaaca tggcgggcgt
     8941 catcccttta ccggcacacg agagcgccaa cagacccagc aaggcgctca cgacattact
     9001 ggagcctaac caggcgctat tgcgaataat ggtgcgaaac gcgccatctg caaaccaatg
     9061 tttgatgtta aaacccgcca attcagcctg accttatcgt tgatagtaaa aagtatcccg
     9121 ccagccttaa gttaaacttc ggcggtcaga aacgatggca accagagaaa ccgccttctg
     9181 tgcctgttcc agcacttcgc tgtagggcgc tctggaatca atctcaagaa tttttgtgcc
     9241 gttatagcca atcgtcgaca taacaccgat tttgtcctgc agctcggcat agtcatggtc
     9301 aggcttgcgg gagatggcag tctcaatatc aatgcccagg cgaataatta attccgggcg
     9361 atattgcgcc atttgttggt ataaacgccg ttcgcgctgc gccagaaaca tgctgatttt
     9421 cccggtcgca cgttcgacgc caatccccgg tccatcataa taaaagcccg aaatttcagc
     9481 ctgcgggaag cgatcgctga ccaccagaac gccactttgc gccagtcgct gaaccttgcg
     9541 tagattcgcc attcttcgca gcgagaagca gtacataatc accgccgccc atagcgccgg
     9601 agatttggtt ttcatgcttt gggttttcga ggatttggcc gccagtcgcc gttccagcca
     9661 gacgccaacc aacggcaatc gttttatttt gtcgccgtct tcgcccgaga gcagccccaa
     9721 atagcgccgc tcggtttgcc agtgttgttg cagcgatttc accaggtcgg tggtcagtgt
     9781 ggatttaccg gtgccatcac acccgaccac cgcaatcagc cccggaatgt agttgggctg
     9841 cggcgcggtt gtactattaa cagtttgtgt ttgtagtgca tccattcgtt aaatcatccc
     9901 ctatccattt tggtgatcag gaaagtgcgg cgtgaattgc gcgtagtgac gcttgcagaa
     9961 tttcatcggc tggttgccga ccatccagtt caaggatctt tgcgccattg aatgtcagtt
    10021 gcggcgtaac ggcgattttt tcctgtaacg ctgccagttg gtggtcaggt ttacgcgcaa
    10081 acgcggtttg ttcatcaatg ccaagacgaa tcaacaatac gggtaaataa gacgccatcc
    10141 attggtacag cttcaactcg cgctgcctta acatttttat ccaaccgtta ccgcccgtgg
    10201 tttttgccaa ttgcgggcca tcaaagcgaa aacccggcac ttcaacctgc gggtagcggt
    10261 cggtgatgag cagatggcct tgctggcttc tacacaacat tttgcgaaac ttgtacgccc
    10321 gccagcagga aagcagatag ataaccagcg cagtaatatt gccaggcggt gttgagggct
    10381 tttcgtgcac atgtgccgct ttacttcgca gataacgccc aaaaggtgcg ccaataacag
    10441 ggagctgtga aatccattcg ccaattcgcc cggacgattg cccgagataa atgcgttctg
    10501 ttggcattct tgctgccagt tcatttacca ggcttgccgt gagggtcgat ttacctgaac
    10561 catcacatcc tacaatggca ataacacgca ctggcgtgga attaattatt gacatattac
    10621 gttgattcac gaaaaacccg gcagtaaata atgtattgaa tattacgtgg tccgtgctca
    10681 gaatatccgt tcaaccttat tgtgacaatg gttaattgtg acattgacct gaattacgct
    10741 ttcataaaaa catattaacc aaataaatat tttggatgga tatttaaatt taaggatata
    10801 ttcatgcagt caataacacc tccattaatt gccgttattg gtagcgatgg ttcaggcaag
    10861 tcaacggtgt gtgaacatct cattaccgtt gtcgaaaaat atggtgctgc cgaaagagtt
    10921 catttaggaa aacaagccgg aaatgtcggt cgtgcagtga caaaattacc gttgatggga
    10981 aaatccttac ataaaacaat tgaacgaaat caggtgaaaa cagcaaaaaa attgccagga
    11041 ccagttccgg cgctggtaat tacagcgttt gtcgcccgtc gcttactgcg ctttcgtcat
    11101 atgcttgcct gtcgtcgtcg cgggttaatt gttctaaccg accgttatcc tcaggaccaa
    11161 attcctggcg cttacgatgg tacggtgttc ccacctaacg ttgaaggtgg tcgttttgtc
    11221 tcatggctgg caagccagga acgtaaagcg tttcactgga tggcgagcca taagcctgat
    11281 ctggtcatca aactcaatgt cgatctagac gtggcctgcg cgcgtaagcc tgaccacaaa
    11341 agggagtcgc tggcaagaaa gatagccata acgccacagt taacctttgg tggtgcacaa
    11401 ctggttgata tcgacgccaa tcagccactg gaacaggtgt tagttgatgc agaaaaagcg
    11461 attacggatt ttatgaccgc gcgtggttat cactagccaa aaatggaaat gcccgatcgc
    11521 caggaccggg catttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
    11581 gaaagaaaaa ttgccgccgg taaagtaaac acccatgcca tcaggatgct ggttaccgtt
    11641 ttacgctgta acccaccgcc gtccaccacc atcgtccctg caactgcaga cgagaggacg
    11701 tgtgttgtag agacgggcat cccaatataa ctggcaagac cgatagacac tgccgccgtc
    11761 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
    11821 gtcatcgcaa cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
    11881 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
    11941 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
    12001 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
    12061 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
    12121 ggatgacagt gaaactccgt tacttgcgtg ccatcagtcg atgccgcagg caacggaggt
    12181 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
    12241 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
    12301 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ctgtccgtcg
    12361 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
    12421 aatggcggct tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
    12481 tcacgctttt tcgtcccgct ccagaaacgg cgcagcagga aaatcagacc acccgcaatg
    12541 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
    12601 tttaacgcat ccatcaccga tgagccggtt aacagtgcgt tggttaaacc gatgccgata
    12661 atcgcgccaa tcaaggtatg cgaactggag gccggtaaac caaagaacca cgttcccagg
    12721 ttccagataa tcgccgctag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
    12781 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
    12841 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggctgc
    12901 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
    12961 gcttcgtaga acaacacaaa tgccagagca agtaataaca aaagccctgt gtatatatca
    13021 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
    13081 cattatcagt gactttaacg gtgtgggcaa agtgaaatat cattttttaa cgataaaaag
    13141 agtgttattt cttgtgtatc agacaataag cattcattaa aaatctttag aaataattag
    13201 aacacaggca ttctgctggt taatgtattc acatatgaaa tgaataaata ttggaatata
    13261 taaatattga atattttgat taatccccgt actgactatt cttcataatc agtacgggat
    13321 tgctacaaca tgtattactc tttcaccaca attaacggtt caatatcact ctcttttttg
    13381 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc aacggtgaag
    13441 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
    13501 tttttctgct cggcaaattt accgctggtt ttgtccagca cttcttcatg atggctgacc
    13561 aggtcgatat tgctaccaca gcgaccggcg atcggtttca ctgcgtaacc tgttttcacc
    13621 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
    13681 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagacc
    13741 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cattttgcgg atgaccggta
    13801 cggatcggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
    13861 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
    13921 ccggcagcat cccagcccag ttcatccagc ccgcgcaaga tacgcgtttc aaagcccgcc
    13981 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
    14041 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccag ttcgttaatc
    14101 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
    14161 atcaagccag cttcggtatg acaggaggcg gagtcggcgt tgtattcgta aaccttcagg
    14221 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
    14281 caggagagac gcaaacgtgg ccagaggatt ttcggaatgt cgaacagcgc cagcaggttg
    14341 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
    14401 ttaatcagct cctgctcggc gctctcggta atggtgtagt aatgatacgg atcctgattg
    14461 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
    14521 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
    14581 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
    14641 atggtggtgt catcaaaagt gtctttaagg gtatagcagc cgttttcgac caccatctcc
    14701 agttcgcgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
    14761 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
    14821 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
    14881 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt cacgcaggaa gcgcagcgag
    14941 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtagttcag aaagagaaaa
    15001 cggcgagcaa attcaacgca ttgccatttg tggcccatat attcgtcgtc gatatagcta
    15061 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
    15121 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
    15181 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc ggaattgttg
    15241 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
    15301 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
    15361 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
    15421 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
    15481 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacatcca ttgcaacttt
    15541 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
    15601 tgggcgttac tggtgcagag tacgacgcct ggctgatacg cataggcgat ggcgatcaat
    15661 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
    15721 cgcataatcc gccgatccgc gtgtttgagt ctggttcgat cctgctttat ctggcagaaa
    15781 aatttggcta cttcttgccg caggatctgg ctaaacgtac tgaagcaatg aactggctgt
    15841 tctggctaca gggtgccgcg ccgttcctcg gcggtggttt tggtcacttt taccattacg
    15901 cgccggtgaa aattgagtac gccatcaatc gctttaccat ggaagccaaa cgtctgctcg
    15961 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgctgag tacaccattg
    16021 cggatatggc gatttggccg tggtttggca atgtggtgct cggcggcgtg tatgatgccg
    16081 cagagtttct ggatgcgggc agttataagc atgtgcaacg ctgggcaaaa gaagtgggcg
    16141 aacgtccggc ggtgaagcgt gggcgtattg ttaaccgcac caacgggccg ctgaacgagc
    16201 agttgcatga gcgccatgac gccagtgatt ttgagacgaa tacggaagat aagcgtcagg
    16261 gataagggtt agcgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
    16321 tccggcaatc gagcacaatg cctgatgcga cgctgccgcg tcttatcaga cctacgaaag
    16381 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
    16441 tggcttcatc ttcgtcgatg atactcatgg caaatccgac gtgtaccagc acccactggc
    16501 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
    16561 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggccag
    16621 gaacgccaat acacattgtt aactccggtt attcaacttc aatacttttg acgatcagcg
    16681 aatcgccggt atcgacacgc aaccgctcgc catgacagat cggacattgc gcatcgtgct
    16741 gatgaatctc caccacctgg ctgcaatccc agcaccaggc ctgggcgggt ttatagacga
    16801 tatgtaaatc gcacccttgc gccaccgttc cctggcagac aatttcaaaa ctaaaacgga
    16861 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
    16921 taacatcatg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagcgaca
    16981 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
    17041 acatccggat tggtgaccgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
    17101 tggccctctt caatcgacat actgtgcgaa agcggtgaca tcagcgagca ggagagatat
    17161 tgagaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
    17221 ccaatttttt cactcacttt acgcagcggc cagagttgat ccgggccggg gaagataact
    17281 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaag
    17341 tcagaaacat acaccggtat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
    17401 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
    17461 tcttcagtca tgaatagcct cccgtgggat agcttccacg ccagattcac gcagcgcagc
    17521 cagaacctgc tcaagcgctg gttcaatcat cgcttcgacc gtcggcgtca agccgatgtg
    17581 cggctccagt gattccggaa ttacgccgac cagggtcagc tttttcggaa actcgccggt
    17641 gaagcgcagg gccgacaaga cgtcggccag gccaagctga tgcggagaga ttttgttggt
    17701 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg cgctcttctt
    17761 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
    17821 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
    17881 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
    17941 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcagcatcc
    18001 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
    18061 agcggtttat tcggatcggc aaccggtgta cccaccagcg actgctcgta aggaccgacg
    18121 tcatcattga agttacgcgg accagagttc caggttgatg gaacaaccgc ctggtagttg
    18181 ctgatgatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
    18241 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gcttcacaaa ggtggtgtga
    18301 tcgcctttgc cgatattggt gatcagtgca ctgtattggt tttgcaggat atcctgcaat
    18361 tcacagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgtgccact
    18421 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
    18481 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
    18541 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
    18601 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
    18661 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
    18721 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
    18781 tttttgctgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
    18841 gtcagccatt ccgggtagaa cgcggcgata accgcggtat caaccttata aacctgttca
    18901 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
    18961 acgcccaaac cgtcgaggtt gattgggtta gcgaccccac ctaccgccag gttctgaatg
    19021 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
    19081 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
    19141 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
    19201 ttgttctgaa ctttggtgaa ctcttccgga ctgttcaggt gccaggtcga aacacctttc
    19261 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
    19321 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
    19381 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
    19441 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
    19501 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
    19561 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
    19621 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
    19681 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
    19741 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
    19801 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
    19861 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
    19921 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
    19981 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
    20041 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
    20101 ggctcttttc atccggaccg ttgccacgca gacccgcctg caccagtgaa ccttcaaaga
    20161 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
    20221 agctctgcca cagcggatgc accttgtagc ccgccgagat catcagcgac cccattgaag
    20281 actggtgcat ggtcggcagc aacgcaccga gcgcgatgat gaagaacatc accttgttca
    20341 gacgctgtag cgacaccttc cagcccagac gttcaaacag tgccggagca aactccagag
    20401 ccatcacgcc gatgtagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
    20461 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
    20521 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
    20581 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
    20641 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
    20701 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cccaatcagg agcatacaga
    20761 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
    20821 ttggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
    20881 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
    20941 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
    21001 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    21061 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    21121 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    21181 atcagctctt cacgcgtacc aaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    21241 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    21301 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    21361 cagtaacggc agccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    21421 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    21481 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    21541 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    21601 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    21661 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    21721 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    21781 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    21841 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    21901 caaccacacc gccgagcaaa ccaatagcgc ccgcagagac gttgccgccc tctttagcgt
    21961 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    22021 ctttatggaa gccgatacct tcttcgttac agccatagca cgggtgacca atggccaccg
    22081 gccacacacc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    22141 ctttacagcc gaggtggtac aggcaccagc cttcgcggtg gccttcatca ccgaactctt
    22201 tggcaaaacg acccgcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    22261 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    22321 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    22381 cggttttgcc tggcagaact tcttgcaagc tgactgcgcc agttgggtta actccagctg
    22441 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatggctgct gcgccttccg
    22501 ccgctttgcg gatgtgatcc acaatcggct caccggcaac catgcaataa ataccgttat
    22561 cttttaatgg gatggaacca tccaccacta acacatactg ccctttgtac ttctcgagag
    22621 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    22681 ccagagagat agtctccagt acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    22741 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    22801 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    22861 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    22921 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    22981 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    23041 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    23101 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    23161 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    23221 gcggcgcgtc agaatacccg cttcccaggc gctaagctgg cgatctccgg tcttttcgta
    23281 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    23341 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    23401 gttattcatc gttcacctca ttcgctatat attgtcatat atagcgatat tttaggcaaa
    23461 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc cgtaagagat
    23521 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    23581 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    23641 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaagccagc
    23701 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    23761 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    23821 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    23881 tgcctgtcgc ttgcggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    23941 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcatta cccggataac
    24001 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    24061 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    24121 gcgttttgcc gctcatcctt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    24181 ttggcgaagg atacgtgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    24241 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    24301 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga gattttcccg tgagcgtaat
    24361 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    24421 cactcctcaa ctggtgtttt tttaagcaaa gcataagcgc gtgtttttgc ccaatttttc
    24481 gtcactctgt gagccagact acgggatacg tgctggcgaa tcgctaaact agaaacattg
    24541 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    24601 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    24661 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg caatcctgcg
    24721 taaagcgttt gatttaggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    24781 aggaagcgca gaagagaact ttggtcgcct gctgcgcgag gattttgccg cttatcgcga
    24841 tgaactgatt atctctacca aagccggata cgatatgtgg cccggcccgt acggctctgg
    24901 cggctcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tggagcttga
    24961 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    25021 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    25081 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctgtt
    25141 aattcatcaa ccttcgtaca atttactaaa ccgctgggtg gataaaagcg gcctgctgga
    25201 taccctgcaa aataacggcg tgggctgcat tgcctttact cctctggctc agggattgct
    25261 gaccggaaaa tatctcaacg gcattcctga agattcacgg atgcatcgtg aagggaataa
    25321 agttcgtggc ctgacgccga aaatgctcac cgaagccaac ctcaacagcc tgcgcttatt
    25381 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    25441 gaaagatgag cgagtgacgt cggtattgat tggtgccagc cgcgcggagc aacttgagga
    25501 gaacgtgcag gcgctgagta atctgacgtt tagcaccgag gagctggcgc agattgatca
    25561 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtct tccgataaat gacctgttaa
    25621 taacgggcgc gagaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    25681 gatagcccat cacaaatgca gagagcacaa acgtcagatg gataatgacg taccacatca
    25741 gtttgttatc agggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    25801 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcatccatt ttccccagcc
    25861 agttcagctt ctctttgttc tcggagatat cgagttgtga gacgaaattc tcataaccgg
    25921 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    25981 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    26041 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    26101 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    26161 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcgcgcaaca gcaaatcaac
    26221 atatccgtta aacctttgtc gcataattct gagggttagg gatcttcctg gttcccttat
    26281 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    26341 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    26401 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    26461 ttggtagcgg tcgcctgaaa gatcgtaaag ccctggtgac agggggcgat tccggaatcg
    26521 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    26581 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    26641 ccgttctgct gccaggcgat ttaagcgatg agaaatttgc ccgttcgcta gttcacgaag
    26701 cgcataaggc cttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    26761 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    26821 tcgcgctgtt ctggctaacc caggaagcgc tccccctgct accgaaaggt gcaagtatta
    26881 tcaccacttc gtcaatccag gcataccagc caagcccgca tttactggac tatgcggcta
    26941 cgaaggcggc gattctgaac tacagccgag gcctggcaaa acaggtcgcg gagaaaggta
    27001 ttcgggtgaa tattgtcgcg ccaggcccga tctggacagc actgcaaatt tccggcggac
    27061 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    27121 cggcggaact ggccccagta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    27181 cagaagtgca cggtgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    27241 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    27301 cctacaaacg catgaagatt caacatgttg caatagattt tgtaggcctg ataagcgtag
    27361 cgcatcaggc attgagcacc taatgccgga tgcggcgtga acgccttatc cggcctacaa
    27421 acgcacgaag attcaacatg ttgcaataga ttttgtaggc ctgatgagcg tagcgcatca
    27481 ggcaattcta ctttacttcg ctttggcggt ttcttcgccg accagaccta tcttcaggta
    27541 acccgcctga tgcagcgtat ccattacctt catcaacgtc tcgtaatcga cggttttatc
    27601 cgctcggaag aagatggtgg tgtctttctt gccttcggtt aacgcattca acgccgtaat
    27661 cattgtttca tcggtgaccg gatcgttacc gataaacatc gagttgtctg ccttcaccga
    27721 cagataaacc ggtttttccg ggcgcggctg cggcgtgctg gtagaagcag gcaagttaac
    27781 cttcacatct accgtcgcta acggtgccgc caccataaag ataatcagca gaaccaacat
    27841 cacgtcgata aacggcgtca cgttgatatc atgcatttcg ccgttatcgt cgaggttttc
    27901 gttaagatgc attgccatcg gacattatcc tgcgcgtaat ttttgtgcga cacgaaccgg
    27961 atgcgcagcg gcgctggctt ccagatccag gtcacggctt tgcagcaaca atacctgcgc
    28021 tgcgacatca cccagcatcg ctttaaagcc gccaatctga cgcgcgaata cgttatagat
    28081 aacgaccgcc ggaatcgctg ccaccagacc gattgccgtt gctaacagag cttctgcgat
    28141 acccggcgca acgactgcca ggttagtagt ctgcgtttgc gcgataccaa taaagctgtt
    28201 catgatgccc cagaccgtac caaacagccc aacgaacggc gaaatcgcgc cgatggttgc
    28261 cagatagccg ttaccgcggc ccatttgacg acctactgcg gcgacccgac gctccagacg
    28321 gaagctggta cgttctttaa taccttcgtt atcgtcgctg ccttctgaca gttccagctc
    28381 attctgcgct tcattgagca aatgcaggct taagcttttg ctaccaaaat cagcggcgat
    28441 atcgttggcc tggtttaagg aacgcgcttc agccagcagt tgctgctcgc gcttaaggcg
    28501 acgcttctga ttgaagaact ctacgctctt actgaagaag attgcccagg tgactacgga
    28561 ggccaaaata agcccaatca tcacgcactt aacgacaata tcggcgtgct gatacatacc
    28621 ccagacggaa aggtccgtct gcattaaatt attacccact gtgtatctcc aggacgcaag
    28681 tcacaaaatc tgcgcataat aatatcaaaa cgacgtcgaa ttgatagtcg ttctcattac
    28741 tatttgcata ctgccgtacc tttgctttct tttccttgcg tttacgcagt aaaaaagtca
    28801 ccagcacgcc atttgcgaaa attttctgct ttatgccaat tcttcaggat gcgcccgcga
    28861 atattcatgc tagtttagac atccagacgt ataaaaacag gaatcccgac atggcggaca
    28921 aaaagcttga tactcaactg gtgaatgcag gacgcagcaa aaaatacact ctcggcgcgg
    28981 ttaatagcgt gattcagcgc gcttcttcgc tggtatttga gagtatggaa gccaaaaagc
    29041 acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg acggcgcgga acgttaaccc
    29101 atttttcctt acaacaagcg atgtgtgaac tggaaggtgg cgcaggctgc gcgctatttc
    29161 cctgcggggc ggcagcggtt gctaattcga ttcttgcttt tgtcgaacag ggcgatcatg
    29221 tgttgatgac caacaccgcc tatgaaccga gtcaggattt ctgtagcaaa atcctcagca
    29281 aactgggcgt aacgacatca tggtttgatc cgctgattgg tgccgatatc gttaagcatc
    29341 tgcagccaaa cactaaaatc gtatttctgg aatcgccggg ttccatcact atggaagtcc
    29401 acgacgttcc ggcgattgtt gccgccgtgc gcagtgtggt gccggatgcc atcattatga
    29461 tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct ggattttggc atcgatgttt
    29521 ctattcaggc cgccaccaaa tatctggttg ggcattcaga tgcgatgatt ggcactgccg
    29581 tgtgcaatgc ccgttgctgg gagcagctac gggaaaatgc ctatctgatg ggccaaatgg
    29641 tcgatgctga taccgcctat ataaccagcc gtggcctgcg cacattaggt gtgcgtttgc
    29701 gtcaacatca tgaaagcagt ctgaaagtgg ccgaatggct ggcagaacat ccgcaagttg
    29761 cgcgagttaa ccaccctgcc ctgcctggca gtaaaggaca cgaattctgg aaacgagact
    29821 ttacaggcag cagcgggcta ttttcctttg tgcttaagaa aaaactcaat gatgaagagc
    29881 tggcgaacta tctggataac ttcagtttat tcagcatggc ctactcgtgg ggcgggtatg
    29941 aatcgttgat cctggcaaat caaccagaac atatcgccac cattcgccca caaggcgaga
    30001 tcgattttag cgggaccttg attcgcctgc atattggtct ggaagatgtc gacgatctga
    30061 ttgccgatct ggacgccggt ttttcgcgga ttgtataaca ttgccacttt tggacaattt
    30121 tgcagacatt ttattgtgaa aagtcttaaa ttgttgcgtc cgggatcaag gcgtcccgga
    30181 cgattcagga gtacaatagg cagataaagg cttaaacgct gttccacagg aaagtccatg
    30241 gctgttattc aagatatcat cgctgcgctc tggcaacacg actttgccgc gctggcggat
    30301 cctcatattg ttagcgttgt ttactttgtc atgtttgcca cgctgttttt agaaaacggc
    30361 ctgctgcccg cctcattttt gccaggcgac agcttgttga tactggcagg cgcattgatt
    30421 gcccaggggg ttatggattt tctgcctacg attgcgattc tgaccgccgc agcaagtctg
    30481 ggctgctggc taagttatat tcaggggcgc tggttaggga ataccaaaac ggtgaaaggc
    30541 tggctggcac agcttcctgc taaatatcat cagcgcgcca cctgcatgtt tgaccgccac
    30601 ggtctgctgg cgctgctggc tggacgtttt cttgcattcg tccgtacgct gctgccaacc
    30661 atggcgggaa tttccggtct gccaaaccgc cgtttccagt ttttcaactg gttaagcgga
    30721 ttgctgtggg tcagcgtggt aaccagtttc ggctatgcct taagtatgat tccgttcgtt
    30781 aaacgccatg aagatcaggt aatgacgttc ctgatgatcc tgccaattgc cttgttaacc
    30841 gctggcttgt taggcacgct gtttgtggtg attaaaaaaa aatactgtaa cgcctgacga
    30901 ttttccctgt tcccggttgc tgtaccggga acgtatttaa ttcccctgca tcgcccgcat
    30961 tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt ttaaactcac ggctaaattg
    31021 cgatgcgctt tcatagccga cgcgcatcgc tgctgcgctg gccttcatgc cgtcatggat
    31081 gatcatcatc cgcgccttat gcagacggta attcttcaaa tactgcaacg gcgaggtgct
    31141 ggtgacagac ttaaaattat ggtggaacgc cgatacgctc atattggctt ctgccgccag
    31201 ttgctcgacg ctcaggtttt cggtgtattt attctcaatc cgtttcagca cgcggctaat
    31261 cagactgaag tgagtctggc gactgaccag cgccagtaac gcgccgccgc aaggtccggt
    31321 cagcacgtag tacagaattt cgcggatgat ctgtttgccg agaatacgcg catccagtgg
    31381 tcgctccatc acgtcgagta accgctccgc cgcgcataaa atctcttctg ataacgtggc
    31441 ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc tcatcttcgc caatgtccat
    31501 caacagttcc tgtaactgca aaatatcgac attgagacgc atccctgcca gcggcacctc
    31561 tgacgtggca taggtttcgc actcaaacgg caacggcacc gtcagcagca ggtattcatt
    31621 ggcatcataa cgaaacacgc gttcattgat ataaccgatt ttatgtccgg aaaagagaat
    31681 tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa ggcgtctcgc catacaacaa
    31741 acgcacatcg ggcaacagtt ctgacaaact attttcttta tttttcagtt tattaacttt
    31801 atccgccagc aagcggcaaa tctcttcacg tttcatatcg cgtaatttct taggaataat
    31861 gcggcaattt gattgtgcgc aattttgtag catttctcca gcactctgga gaaataggca
    31921 agacattggc agaaatgagc attgagagcc agggcgctgg cgatcacaat gaaaaacatc
    31981 aggcagatcg ttctctgccc tcatattggc ccagcaaagg gagcaagtaa tgaacaactt
    32041 taatctgcac accccaaccc gcattctgtt tggtaaaggc gcaatcgctg gtttacgcga
    32101 acaaattcct cacgatgctc gcgtattgat tacctacggt ggcggcagcg tgaaaaaaac
    32161 cggcgttctc gatcaagttc tggatgccct gaaaggcatg gacgtgctgg aatttggcgg
    32221 tattgagcca aacccggctt atgaaacgct gatgaacgcc gtgaaactgg ttcgcgaaca
    32281 gaaagtgact ttcctgctgg cggttggcgg cggttctgta ctggacggca ccaaatttat
    32341 cgccgcagcg gctaactatc cggaaaatat cgatccgtgg cacattctgc aaacgggcgg
    32401 taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg acgctgccag caaccggttc
    32461 agaatccaac gccggtgcgg tgatctcccg taaaaccaca ggcgataagc aggcgttcca
    32521 ttctgcccat gttcagcccg tatttgccgt gctcgatccg gtttatacct acaccctgcc
    32581 gccgcgtcag gtggctaacg gcgtagtgga cgcctttgta cacaccgtgg aacagtatgt
    32641 taccaaaccg gttgatgcca aaattcagga ccgtttcgca gaaggcattt tgctgacgct
    32701 gatcgaagat ggtccgaaag ccctgaaaga gccagaaaac tacgatgtgc gcgccaacgt
    32761 catgtgggcg gcgactcagg cgctgaacgg tttgattggc gctggcgtac cgcaggactg
    32821 ggcaacgcat atgctgggcc acgaactgac tgcgatgcac ggtctggatc acgcgcaaac
    32881 actggctatc gtcctgcctg cactgtggaa tgaaaaacgc gataccaagc gcgctaagct
    32941 gctgcaatat gctgaacgcg tctggaacat cactgaaggt tccgacgatg agcgtattga
    33001 cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta ggcgtgccga cccacctctc
    33061 cgactacggt ctggacggca gctccatccc ggctttgctg aaaaaactgg aagagcacgg
    33121 catgacccaa ctgggcgaaa atcatgacat tacgctggat gtcagccgcc gtatatacga
    33181 agccgcccgc taagcttttt acgcctcaaa ctttcgtttt cgggcatttc gtccagactt
    33241 aagttcacaa cacctcaccg gagcctgctc cggtgagttc atataaagga ggaacgtatg
    33301 gctaatccaa ccgttattaa gctacaggat ggcaatgtca tgccccagtt gggactgggc
    33361 gtctggcaag caagtaatga ggaagtaatc accgccattc aaaaagcgtt agaagtgggt
    33421 tatcgctcga ttgataccgc cgcggcctat aagaacgaag aaggtgtcgg caaagccctg
    33481 aaaaatgcct cagtcaacag agaagaactg ttcatcacca ctaagctgtg gaacgacgac
    33541 cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa aactccagct tgattatatc
    33601 gacctctact taatgcactg gcccgttccc gctatcgacc attatgtcga agcatggaaa
    33661 ggcatgatcg aattgcaaaa agagggatta atcaaaagca tcggcgtgtg caacttccag
    33721 atccatcacc tgcaacgcct gattgatgaa actggcgtga cgcctgtgat aaaccagatc
    33781 gaacttcatc cgctgatgca acaacgccag ctacacgcct ggaacgcgac acacaaaatc
    33841 cagaccgaat cctggagccc attagcgcaa ggagggaaag gcgttttcga tcagaaagtc
    33901 attcgcgatc tggcagataa atacggcaaa accccggcgc agattgttat ccgctggcat
    33961 ctggatagcg gcctggtggt gatcccgaaa tcggtcacac cttcacgtat tgccgaaaac
    34021 tttgatgtct gggatttccg tctcgacaaa gacgaactcg gcgaaattgc aaaactcgat
    34081 cagggcaagc gtctcggtcc cgatcctgac cagttcggcg gctaacgtgc aaattctccc
    34141 ggtggcggta atgttccgct accggacttt tcagaaatca tttattcccc tcgcgtcccg
    34201 cccgttgtta ctcttccttg ttcaggaatg ccaaatataa ggacatcatc atgcagagcc
    34261 ggaagctctt aaaagaacaa ctcatctata tccgggataa acgcaacgga gaggtgaaaa
    34321 acagatgaaa ataatacttc tgtttttagc agccctggca agttttaccg tgcacgcaca
    34381 gcccccctca cagaccgtag aacaaacagt ccggcatatt tatcagaact ataaatcaga
    34441 tgccactgcc ccttattttg gtgaaaccgg agagcgggcg ataacttctg cgcgtattca
    34501 acaggcgctt accctgaacg acaatcttac gctgccgggc aatattggct ggctggatta
    34561 tgatccggtt tgtgattgtc aggattttgg cgatctggtg ctagaaagcg tagcgataac
    34621 cgaaactgac gccgatcatg ccgatgccgt tgtgcgcttt cgtatcttta aagatgataa
    34681 agaaaagacc acgcagacac tgaaaatggt ggcggaaaat ggtcgttggg tcattgacga
    34741 tattgtcagc aatcatggca gcgtcttaca agcagttaat agcgagaatg aaaaaacgct
    34801 ggccgcttta gcttcgttgc aaaaagaaca gccggaagcc tttgttgccg aactctttga
    34861 acatattgcc gattatagct ggccgtggac gtgggtggtt tccgactctt accgccaggc
    34921 ggtcaatgcc ttctataaaa ccaccttcaa ggcggccaac aatcccgatg aagatatgca
    34981 aatagaacgg caatttattt acgacaatcc gatctgtttt ggcgaagagt cgctattttc
    35041 acgcgttgat gaaattcgag tcctggagaa aaccgtcgat tccgcccgca ttcatgttcg
    35101 ttttacgctg accaatggca acaacgaaga gcaagagctg gttttacagc ggcgcgaagg
    35161 taagtgggaa atcgctgatt ttatccgccc gaacagcggc agcctactta agcagattga
    35221 ggcaaaaacc gccgccagat taaagcaatg agctgaatta aataacaatt agccggaaca
    35281 ataaaaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac
    35341 ctgcgtgctc agtggctgcg tcaatgagca aaaggtcaat cagctggcga gcaatgtgca
    35401 aacattaaat gccaaaatcg cccggcttga gcaggatatg aaagcactac gcccacaaat
    35461 ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgcac aggactattt
    35521 tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt
    35581 cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag
    35641 cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct
    35701 gggtcgccat cggcgtatgt ttcgtcaacg ccggacgggt attgcggttc tggcgacgag
    35761 cctcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca
    35821 gatgcttttt gcccatcgct tccagcgcct ggcggattaa cggccagttt gccggatcgt
    35881 ggtaacgcaa caacgctttg tgcaaacgac gctgtttgtc acccttcggt acaaagacgt
    35941 cttcactctt ataaccaatc ttcgccagcg ggtttttccc ggtgtaatac atggtggtcg
    36001 agttagccag cggcgatggg tagaaattct gtacctggtc gagacggaaa cgatgctttt
    36061 tcagccacag cgccagattc accatatctt catcacgcgt acccgggtgc gcggagatga
    36121 aataggggat cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt
    36181 taaagcggtc ataactgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg
    36241 tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccagt tctttgatat
    36301 agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcaatcaga atctttttaa
    36361 tgcctttcag atcacgcgca cggcgataga ggttgatcgt tggttcgtgg ttcgtgtcca
    36421 tatgcggaca aatatccggg taaacgcagg acaaacgacg gcaggtttgc tcagcgcgtg
    36481 gcgatttgca gcgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc
    36541 ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg
    36601 aacggctctg aataatgcgc ccttcgtgct cggtgataga acagaaagag cagccgccaa
    36661 agcagccgcg cataatgttg accgagaaac ggatcatttc gtaagccgga atacgggcat
    36721 tgccataggc cggatgcggc acgcgcttat acggcagcgc aaaaacgctg tccatctctt
    36781 cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcgccg tgtttttgca
    36841 tcaatgcgcg ggcacagcct gggttggttt cgtggtgcag aatacgcgaa gcatgggcgt
    36901 acagcacttt atcgcccttc actttctcga aagaaggcag caatacgtag gttttttccc
    36961 atggtttcgg gcgcggcggc tgcacagtta cagctttagc ttcctgcttt ttcggggcca
    37021 ccggtttgtt atccgcacac ggcaaatctt cgccatacgg atgcgggatc gggtcgattt
    37081 tcccaggggt atcaagacga gtagaatcca caccgctcca gcctggcaac gcctctttta
    37141 cgataatcgc agtattacgc acatcgcgga tttcgctaat tggctcgccc atcgccagac
    37201 gatgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg tcggctttcg
    37261 aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgc gcggtacggc
    37321 gcagactagc ctcaatacca ccgaggatca ccggtacatc tttccacgcc tctttacaac
    37381 gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta tccggcgtgt
    37441 aggcatcgtc atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc gaatccatgt
    37501 tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag tcgtctttgc
    37561 tgctccagtc tggctgagcg atgatcccaa cgcgaaagcc ctgtgcttcc agcatacgac
    37621 cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga
    37681 tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg
    37741 ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga
    37801 tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg
    37861 attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct cctcgggcga
    37921 gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac ggtccgccat
    37981 ttccagcgtc tgactgttga agtagaacgg aaagtgcgcc caggaaggct gaccgaaata
    38041 aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg
    38101 gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa tatggaacgc
    38161 ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc
    38221 aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc
    38281 gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaggcgca tcggaagact
    38341 gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg tagaaaccag
    38401 aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcaccaca
    38461 ggtgatcgac acttcatcac cgttgctcat atccaccaga atctcgcggc gctcgcccgg
    38521 tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat
    38581 cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt
    38641 cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt gcacaccgtt
    38701 aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc
    38761 aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacac cataatggtt
    38821 ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt
    38881 atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt
    38941 ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc
    39001 gcccatcagc gggcctggta cctgtagccc ggccaccgtc attgagacat tttctgtcag
    39061 gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag
    39121 gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc
    39181 acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagcagcg gcggaacggg
    39241 tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc
    39301 aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac
    39361 tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa
    39421 acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc
    39481 attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg
    39541 tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc
    39601 ttattcgaag ttgtagagac gcacacggga ataatcggga cgcctgctgc aattgccgcg
    39661 tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg
    39721 aacatccaaa tggaaatacg gcgttttttg aaatgattca ctacttccgc aatagtgccg
    39781 tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc
    39841 ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca
    39901 atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg
    39961 ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagcggcgca
    40021 agacgaccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg
    40081 cagtaaatgg agccgaatac acagactaag atgctgtaaa taacggtaat aataagacga
    40141 aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa
    40201 atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc
    40261 atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg
    40321 gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca gcgccctccc
    40381 cggcatatta ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac
    40441 gatcgatacg ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagtgactt
    40501 tctgtagctc ttccggacgc agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc
    40561 tttgcggcgg cagaacgtac agttgcgcca gaccatcctc gccacgcgcg gcttctgccg
    40621 atggaatgtt gataatcttg ttgcctttgc ccttcgacag ctgcggcaga tcacttaccg
    40681 ggaacatcaa catacggcct gcctgagtga ttgccagcag catatcggaa gcatcttcaa
    40741 ttaccaccgg cggcataaca tgggcatttt ccggtaaggt gatcaaagcc ttacctgcac
    40801 ggttacgcgc caccagatcg ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca
    40861 tcagcagttt ctgatcgtcg ctttccatca gcatatggtc aacggtcgcc ccaggcggca
    40921 acgttaattt gccggtgagc ggttcgccct gaccacgcgc cgacggcagc gtaatcgggt
    40981 caatggcata gctacgaccg gtggaatcaa caaacactac cggttggttg ctcttacctt
    41041 tcaccgccgc tttgaagcta tcacccgctt tataattcag gcccggcgcg tcgatatcat
    41101 ggcctttagc gctgcgtacc cagcccatct gcgacagcac aatggtgaca ggttcagacg
    41161 gcagcatgtc gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac
    41221 gatcgtcacc gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg ttattcattt
    41281 tacgctcgga agccaaaatg ccctgcaact ggtcgcgctc tttttccagt tcactctgct
    41341 caccgcgaat cttcatctct tccagtttgg caagatgacg cagtttcagt tcgaggatcg
    41401 cttccgcctg ggtttccgta aggccaaacc gcaacatcag cgccggtttc ggttcatctt
    41461 cattacgaat gatctcaatc acttcgtcga tattgagaaa cgccaccagc aaaccttcga
    41521 ggatatgcag gcgcttgagg actttctcca gacgatagtt cagtcggcgg cgcacggtat
    41581 cgcggcggaa caccagccat tcggagagga tttccagcag gtttttcacc gccggacgac
    41641 catccagacc gatcatattg aggttaatgc gatagctctt ttccagatcg gtggtagcga
    41701 agaggtggtt catcacctga tccatatcca cgcggttgga acgcggcaca atcaccagac
    41761 gggtcgggtt ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc agctttttgt
    41821 tgcgcatttg cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga tgcggcaatg
    41881 cgctgataac caccgcgcca tcttctttct tccacaccgc gcgcatacgc actgaaccac
    41941 gtccgttctc gtagatttta cggatctcgg cgcgcgaagt gataatttcc gcttcagtcg
    42001 gataatccgg cccctgcacg atatccagca gctgatcgag cgtggttttc ggctggtcga
    42061 ttaatgcgat tgccgcctga gccacttcac gcaggttatg cggtggaata tcggtcgcca
    42121 tgccgacggc aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg gcaggtagca
    42181 ttttcggctc ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca
    42241 gctcgctcaa tagcagctcg gaatatttcg acaaccggga ttcggtgtaa cgcattgccg
    42301 cgaacgattt cggatcgtcc ggcgcgcccc agttcccctg accatcaacc agcggataac
    42361 ggtaagagaa cggctgcgcc atcaggacca tcgcttcata acaggcgcta tcgccgtgcg
    42421 gatggtattt acccagtacg tcaccgacgg tacgggccga ttttttaaat ttggcgctgg
    42481 cattcaggcc cagttcagac atcgcataca caatgcggcg ctgaacaggt ttcagaccat
    42541 caccaataaa cggcaacgca cggtccatga tcacgtacat ggagtagttt aagtaggcgt
    42601 tttccgtaaa ttcatgtagc gcaaggcgct ctgccatatc gctcattaat tctgattcct
    42661 caacttattc gccagccttc aaaggggcaa tattgccgga gatactacat tatctgccgc
    42721 gtcgagtcac aggagatcag cgtgaatagc gccgccggac gtgctccgac ggcgataaag
    42781 gcatcaatgt gccttgatat acaactcttt gctgtagaca taatcctggg gattatgcag
    42841 cggaaaaccg ccaacgtagg gtttaagcag tttgattaac ggctgatagt agataggaat
    42901 cagcggtgcc tgttggttga tgatcacttc tgcctgctga tacaacgcat tacgctttgt
    42961 cgcatcagtg atctgcgtgg cctggtttag taaggcgtca tactgtgcat ttttccagtg
    43021 accgacgttt tcttcactat cgcttttgag cgtgttcagg aagctggaag catcattgta
    43081 cgtcgcatcc cacgactgcc gagacagcat gaaatcaccg gctcgtcggg catcaagata
    43141 ggttttccac tccattgtgc gcagcgtcac ctgtgcaccc agccattttt tccattcgga
    43201 agacaacgct atcgcgatct tttcatgcag atcgtacttg ttgtagaaca gctcaaagca
    43261 tagcggatga gaggcgtcgt atcccgcctg tttcagcaag gcttttgcca tcgcgacgcg
    43321 ctcactcatt ggcttttgca gttcatcgaa cgtcgtcgcg ctaaagcctt ttacctctgg
    43381 cggcgtcagc gtggttgcgg gcgttctcaa ccccagtacc ttttgcgcaa taagctgtcg
    43441 atcaaccgta agatatagcg cccgacgcac ccgcacatcg ttaaatggcg gtttctcaag
    43501 gttgaagttg taatattcgc tgttcagacg cggaataatt cgtagctcgc caggcagtga
    43561 tttttcaatg gcgggaattt gctgcgccgg aacccaggtg agatcgacct ctcccgcgcg
    43621 atagcggtta tagccggtga ccgaattatc cagcgccaga tactcaacct gttgcaatac
    43681 tgtatgttgc gcatcgcggt actttggatt tttgcgtgca gtaatctttt cgttaactac
    43741 ccactgatca agcacaaagg caccgttgta aaccatgttc tctggcttac tccagctatc
    43801 gccatgttta gcgatgacat gatgaggaac cgggaacagc gtcggccagg cgagcatcgt
    43861 cgtaaaccaa ggaaccggct gctcaagcgt aacttcaaga gtacgatcat ccgtcgcttt
    43921 gacacccagc gatgtaacat ctgctttacc cgcaacaata gccgcggcat tgttaatgtg
    43981 cgcctgtgcc agatagccag caaaagggct tgccgttttc gggtcaaccg cgcgctgcca
    44041 gccgaggaca aaatcctctg ccgtcagagg ctgaccgtct gaccactgca aaccgctacg
    44101 cagatggaaa atatagcgcc tgccgccgtc cagtatctcc cagcgttcag cctgagcggg
    44161 ctgcacctgg ccttcaccgt ccatccatac cagaccttca aacagatcca gcacaatctg
    44221 cgcggcagta ttctcctcca ccttttgcgg gtcgagcgta cctgggtcgc tatgattgtt
    44281 gtaacgaaag acttgttgcg gagcgagcgg tgtgttggtg ggaacgtcag cagcgtagag
    44341 aggagccgca cttaccaggc tgaccagcca taataaattt cgcgtataca tggtattcct
    44401 taccggagaa attataagcc caacaacaat acgcctgtgg cattgttcgc tcaaacttat
    44461 cgcgagtgat ttggctcaca ctccggtaaa gattccccat cacgctaacg gcacataaac
    44521 atccgtttcc agtcgctgct ccgtcacccc ttcggcaaga ttggtgtagt gaaacagaat
    44581 cggtgcttta cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat
    44641 cgtgtgtgaa atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccgat
    44701 aagttcacca ttactcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca
    44761 gatatcaaag cgaaacgctt ctggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa
    44821 agtctggctt tggttaactg gagaaagccc cgtctcttta cgccacatga taaatttcgc
    44881 cacgctgtag ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga
    44941 tgggaaatcg ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat
    45001 tattcccggg aacgcatccc gggagcggta acaattacgg atttactttg cggatctgtt
    45061 tgacgtcaat ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat
    45121 ctttcggcgt caccgtcacg ccgttccagc gtttgtggtc gatatcaaca ttgatagtac
    45181 cgctggcatc tttgaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca
    45241 gggttaccca ggtgtcgtca cgcagggatt ttgcgttttc tacagtcgtt acgctgccgt
    45301 tcggcccctg gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct
    45361 gctctgctgc catcaccggt gcgctgcaca gggccattac tgcgattact gctgcgaatt
    45421 ttttcatgtt tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta
    45481 acaacttctt aagggaaaaa aataaaattt agtgctgtac agagcgcgtt acaacacggt
    45541 ttactggcag caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag
    45601 atgacatgct gattggcgac ggcatcaaaa cgggccttag taaaatgggt tttagcgtcg
    45661 actggtttac acaaggttgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg
    45721 tgatcctgga tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag
    45781 aaaaaggtca gcgtgagccg gtactgatcc tgaccgcgcg cgatgcgctg gaggaacgtg
    45841 tagaagggct gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag
    45901 tcgccgccag gctggaagct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc
    45961 gccacggtaa cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct
    46021 taatactgaa accaaaagaa tttgctctgc tggaattact gatgcgtaac gctggtcggg
    46081 tactgccgcg caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta
    46141 atgccgttga agtgcatgtg catcatctgc gacgcaaact cggcagtgat tttattcgta
    46201 ccgtgcatgg tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct
    46261 gcgtgtcagg ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag
    46321 ctttgtcgcc tggaaacaaa caacggataa cgtcgatgag ttgttcgaca cccaactgat
    46381 gctgtttgcc aagcgattaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat
    46441 ggcacagacg ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc
    46501 catctttacc cacgacggca gaatggtcct taacgatggc gataacggag aagatattcc
    46561 ctatagctat caacgggaag gttttgctga cgggcaactg gtgggcgata aagatcaatg
    46621 gcgttttgtc tggatgacct cacctgatgg caaatatcgc atcgttgttg gtcaggaatg
    46681 ggaataccgt gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggttggt
    46741 cgcactgccg attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct
    46801 gaacaagctg gcgctggcgc tacgtatgcg tgaccctgat tcggaaaaac cactaaacgc
    46861 gactggcgta cccagcgaag tgcgaccact ggttgagtcg ctaaatcaac tgttcgtccg
    46921 cacacatgcg atgatggttc gtgaacgacg ctttacctcc gacgcagctc acgaacttcg
    46981 tagcccgtta acggcgctga aagtgcaaac cgaagttgcg cagctctctg aagatgatcc
    47041 gcaggcgcga aaaaaagcac tgcttcaatt acattacggg atcgatcgcg ctactcgtct
    47101 ggttgatcaa ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc
    47161 ggagatcccg cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca
    47221 gcaggcgaaa attgacgtgc gactgacact caatgtccag ggcatcaaac gcaccggaca
    47281 accgctattg ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gttacagtcc
    47341 acagggcagc gtggtggacg tcacgctgaa tgccgataat ttcatcgtga gggataacgg
    47401 ccccggtgtg acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg
    47461 acaaaccgct accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca
    47521 tggcatgaat gttgagtttg gtaatgcgga tcaaggtgga tttgaggcga aagtaagctg
    47581 gtaagggtaa gacttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg
    47641 taccgctctg tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca
    47701 acatcctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca
    47761 ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtt cgcatcgttc
    47821 gcgccgacag cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg
    47881 tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatacattg
    47941 atgatgtatt caccgaaggt cacgggactc tatatgccag cgatggtcgt acccgcaaag
    48001 atccgtcgaa aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttctc
    48061 taacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc cacggcgttg
    48121 gcgtcgacgg tgtgtatctg ccgttccata aagcaaacca attccttggc atggaaccgc
    48181 tgccgacatt tatcgctaat gacgtgataa aaatgcccga tgttccccgc tatactgaag
    48241 aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt tagaaggagt
    48301 taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg
    48361 cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg
    48421 gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggact
    48481 ctatcgtgat gattgagcag tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc
    48541 cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg aatatccgta
    48601 ttctgcaacc ggggatttaa cattcctgaa aagaggccgg tgttgctacc ggcctcaggt
    48661 cagattagcc attctttctt cccgttttca gcccccgatg gaaatcgtta attcgcttaa
    48721 tggttttaac cgcacgctgc ggcaccgcgg aagccaccga ccagacaatc atttccaggc
    48781 acaacaatac cgtaccatgt agtggcgttt tccccttttc atccccgcgc ggaacatgaa
    48841 tcacgatact ggcgtctttg ctaaaacgtg aatccagcgc gtttgtcagc aaaataacag
    48901 ggatccccag ccgtctcgct tcacgcagcg ttgtcagccc ttcccggtgc gcagattttt
    48961 gcgccatcat gatcagaaca tcgccacgtt gaagcgcgat cagttgctcg gcaaggccaa
    49021 tacccgtacg gtttagtgcc gtggcaggca agcctatgcg gttgaatagt cgtgcggtat
    49081 agtccgccag aatacccgaa gcgccaatgc caaaaattgc gacttgccgt gcctgcacca
    49141 acaacgctac cgcttgcgca agcgcgtggc ggttttcagg ctctgacagg acattacaag
    49201 tatacaaatg cccttcaaga acaaagtcga ttgcggtgtt aacatcgctc gtcaagttac
    49261 tcaccgtcgt agacatcttt tcgctggaac taagggctgg accaagccac tgttccagag
    49321 tacgtttcag atcacgcagc ccggcaaacc ccagagcctg tatagccctg ataactgtgg
    49381 catcggaagt gttcaacgtg gccgcaattt ccatcgccgt ttgctcgagt accacttcac
    49441 gatgctcatt aatgtagctt gcgacagctc ttaacccagg cgttaactga ctcgcgcgag
    49501 tgcgaaaacg ctcaccataa atatcaaccc gcgttttgtc tttctggatc atttagcctc
    49561 cggtagaggt cgtcccgcaa tttgtgattg tatctgcgca gccatcagcc ccagagatgc
    49621 aaaggagtta gaaatcgcct cttcacgaga aatcagcaca tagcgcccgg aacgacaggc
    49681 cgtcagaaac tgacaccatc ccggcatgac tttttccatt gctgccagct catcctgagg
    49741 tttaccaccg gtgtcaccgc gccaggttgc aaaaacaaaa tcggcatcca gttcaggtaa
    49801 acgctctgcg ctcacatcca ttcgcccacc ttcaggaatg ctctcaatca gcggcggaaa
    49861 gcgaaaacca gcatcgcgta atacccgccc cagcgaatga tagctatgca tcacattaat
    49921 ttttccctga tttgcctgaa tcaccgatac cgttattttt tggctgtcca gcgtcgcctt
    49981 cagcgcattg atttgtgcct gatagcggcg ttcaagtatc gccagctgcg actgcgttcc
    50041 tgtaagttct gccagcttgc ggtagatttc tggcgcgcct cctttgagat gatcaatgct
    50101 caccgtgggc gcaatttttt ccagttgttc aatgggcgta ttacgggtcg gttctgtgat
    50161 gatcaggtca ggctttgccg ccacaatcgc ttcaatatca atatcagcgg tgccaataaa
    50221 agcgatcgat gaattatcaa agtcgacacc tgtcaataac gcgcctgatc gaatgaaatg
    50281 gctaccgtca ggccgagttc gcccgtggct ggcgacagga ggtacaccga gctcaatcag
    50341 aggaatagtg atatcaagat cgtgtaaaga gacaattctc tttggatgag caggaacagt
    50401 gactttgcgc cctaaatcat cagtaaagac ctgaacaggt tctgtcgcgc gggcaaagaa
    50461 tgacaacagt atcagcagag aaaaaaataa acgcatataa ctcccattaa aaactgttcc
    50521 tgcgctgcca gagcagtagc aaaaagaatg gtccaccaat caatgaaata atgattccgg
    50581 caggcagttg caaaggcaaa aaagccaaac gaccaatgct atccgccagg atgactaaca
    50641 acgcccccga tacggcgctc cccagcagta atgtcgtctg cccgccacgt agaataaaac
    50701 gtgatatatg gggtgctatc aatccgacaa aaccgatgtt accaacacac gaaacgcaga
    50761 cggctgtcag gattattggt gcggcgacac gtaacaacgc cagacgagaa ctattcaccc
    50821 ctaacccggt tgcaacctga tgaccaagca gagcgatatc cgccgtcctg gcggtgaaca
    50881 gcaataaaag agcggctggc aacatccaac aggctgaaat accgactaac atccagttag
    50941 ctgcatgcag gcttcccgat aaccacatca atgcagtttg tacatctcga acatctgcag
    51001 tggtcataaa tacaccgata ccggcggcga aaaaccagga aacaccaata ccaatcagaa
    51061 caaatcgcgg gcgggaaatt tcgcgtgcac aaaaaatcac gattaaagcc accaacaagc
    51121 caccagcaag accagccact ggacgccaga acattcccag cattggaaac tggaaaatca
    51181 gccacagcac cgccacgcta catccttctt tgacgccgat taaaccagga tcggcaaggc
    51241 cgttacgcgc aatggattgc atcgcggccc ccgccatacc taacatggct ccgcatagca
    51301 atgccatgat gactctcggc aagcgaatat cctgcacaat ataacgtgcg tctgtgctta
    51361 cgttttctgg agcaaacaac gctcgtccta cttccgatgc cgggacagaa aaagaaccat
    51421 gcatcagacc gaatatcagt attccaaccg caaacagaaa cagcccacca agcaacacca
    51481 gatttttagg gcgtaccagt gtcgaaaact taccaacacg tagtggacgt agcccgacgt
    51541 tcattatccg actcatttga acatcctcgt cgccataatg acaaaaacag gcgcaccaac
    51601 taacgccgtc atgacaccgg tcgctaactc atggggcgtg aaaagcgtcc gagcaataat
    51661 atctgccagt aataacaaaa gcgcgccgac acaagctgaa agagggagca gaacgcgcag
    51721 atccgctgat accagacgcc tgacaatctg cggtactagc agaccgacaa aaccaatggg
    51781 gccagcaatc gatacggctg cgccacagag caacgcgatc gcaagcagag tgaatgttcg
    51841 cgtgcggagt accgagacgc ccagcccctg cgccatgcga tcacccagtg ccaacatgtt
    51901 aagagagggc gcaaggtaaa tggctaacac aacccccccc agagaaaacc aggcggacgt
    51961 acccagcgta gcccaatcct gccccgctaa atcacctgct agccatgtcc gcatcgccag
    52021 cagtgtttgt tcgtcaagaa tcaggattgc agccgttatt gaggacacga acgccgatag
    52081 cgccacgccg cataaagtaa ctttcattgg cgttaaccct gaacgccctg cggaggaaag
    52141 cagcaatatc aacaggaaca ataacgcgcc gcccgtagat gccagcagtg ggcgtccaac
    52201 cggaaaggcc agcccaagcg cactggcagc aacgacagca agagcggctc cggcatttaa
    52261 gcctaaaata tgtggctccc ccaatgggtt acgaatgaca gcctgcaaca aagcaccagc
    52321 aactccaaga gaagcaccgg taagcaatgc tgccgctaaa cgcggcaaac ggagtcttac
    52381 aattacgttg tgatcgaagt tttgcggttc aaaatgaaga aatgcctgca acaccgtttg
    52441 tggtgcaata aatcgggcac caatccctgg atataccacg catcccacca gcagtaaggc
    52501 gaggaaaatt acagtaacca gccctgtgcg catcatactt tttctacacc acgaaacggc
    52561 atgaagaacg gctttcccgt cagaggattt attgatgcgt gaacatcaac gtcaaagaca
    52621 gcttttacca gctcaggcgt gcaatgctcc ccctcattca acacacgcac cactttcccc
    52681 tggcgtaaaa atatcaacgt atcaccataa ttgacggcaa aattcagatc atgcaacacc
    52741 acgacgactg tacgcccgtg atgacgcgtt aaatcatgta ataactcaag aatttcaacc
    52801 tgataacgta aatcaagcca ggttgtgggt tcatccagta ggatataagg cgtcttttgc
    52861 gccaacacca tcgcaatcca gcaacgctgg cgctgcccac cagagagttt ttcaacaggt
    52921 aaatgagcaa actcctgcgt accggttaat ttcagggcct cttctaccgc ctgctcgtcg
    52981 gcgtcgctcc attgacgtat gaaattttgc cagggaaaac gcccacgcga caccagttca
    53041 taaacggtta agccctccgg caataatggc gactgcggca agatcccaag tctacgggat
    53101 aacgctttcg ttggttgctc gtgaatcgct ttaccatcca gcaatattga gccgcccatg
    53161 ggttgcaaga tacgtgcaat ggtgctgagc aacgtcgact ttccacagcc gtttgcccca
    53221 accagaacgg tcattttttc tgtgggaacg gagaaagaaa caccatcaac aatgattttc
    53281 ttgtgatacc tggcagaaac attgtcgagg atcagcccct gcccttttgc gttatgtgcc
    53341 acgtgtacgc caacttaacc aaagacaaac aaaaataaaa ctcattctct tttgagagtt
    53401 tgacgccaca aaatgtagta gtcaagaagt taaaagcca
//
LOCUS       DAPGRN010000018        51475 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPGRN010000018 DAPGRN010000000
VERSION     DAPGRN010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.018"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..730
                     /locus_tag="RHW33_001196"
     CDS             29..730
                     /locus_tag="RHW33_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1644721.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIDGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            755..3355
                     /locus_tag="RHW33_001197"
     CDS             755..3355
                     /locus_tag="RHW33_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV1644722.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACALLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQERLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPINLLLPRSNDSYTSKKNYAWMTSNTSIDNEGHTTQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     AISGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGVQTNSGIIDDDGSLYMAGLPAKGTITVRWGEASDQICHISYELTEQQINS
                     AITRMDAICR"
     gene            3346..4416
                     /locus_tag="RHW33_001198"
     CDS             3346..4416
                     /locus_tag="RHW33_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644723.1"
                     /translation="MQIIFGEKCVSLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTTGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGGDTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4428..4970
                     /locus_tag="RHW33_001199"
     CDS             4428..4970
                     /locus_tag="RHW33_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644724.1"
                     /translation="MKKKTIFQCVILFFSLLNIHIGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4978..5493
                     /locus_tag="RHW33_001200"
     CDS             4978..5493
                     /locus_tag="RHW33_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1644725.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTSDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5459..6196
                     /locus_tag="RHW33_001201"
     CDS             5459..6196
                     /locus_tag="RHW33_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV1644726.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNVGVVPPKSLRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6307..7317
                     /gene="pyrD"
                     /locus_tag="RHW33_001202"
     CDS             6307..7317
                     /gene="pyrD"
                     /locus_tag="RHW33_001202"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV1644727.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7491..8033
                     /gene="zapC"
                     /locus_tag="RHW33_001203"
     CDS             7491..8033
                     /gene="zapC"
                     /locus_tag="RHW33_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV1644728.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTNEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8030..9139)
                     /gene="ycbX"
                     /locus_tag="RHW33_001204"
     CDS             complement(8030..9139)
                     /gene="ycbX"
                     /locus_tag="RHW33_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV1644729.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PEAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILTTAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9383..11491
                     /gene="rlmKL"
                     /locus_tag="RHW33_001205"
     CDS             9383..11491
                     /gene="rlmKL"
                     /locus_tag="RHW33_001205"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV1644730.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11503..13410
                     /locus_tag="RHW33_001206"
     CDS             11503..13410
                     /locus_tag="RHW33_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1644731.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKNEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13540..14793
                     /gene="pqiA"
                     /locus_tag="RHW33_001207"
     CDS             13540..14793
                     /gene="pqiA"
                     /locus_tag="RHW33_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV1644732.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14798..16438
                     /gene="pqiB"
                     /locus_tag="RHW33_001208"
     CDS             14798..16438
                     /gene="pqiB"
                     /locus_tag="RHW33_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV1644733.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16435..16998
                     /gene="pqiC"
                     /locus_tag="RHW33_001209"
     CDS             16435..16998
                     /gene="pqiC"
                     /locus_tag="RHW33_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV1644734.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17254..17421
                     /gene="rmf"
                     /locus_tag="RHW33_001210"
     CDS             17254..17421
                     /gene="rmf"
                     /locus_tag="RHW33_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV1644735.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17491..18009)
                     /gene="fabA"
                     /locus_tag="RHW33_001211"
     CDS             complement(17491..18009)
                     /gene="fabA"
                     /locus_tag="RHW33_001211"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV1644736.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18078..19838)
                     /locus_tag="RHW33_001212"
     CDS             complement(18078..19838)
                     /locus_tag="RHW33_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV1644737.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     NAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPILIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADMPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20024..20476
                     /gene="matP"
                     /locus_tag="RHW33_001213"
     CDS             20024..20476
                     /gene="matP"
                     /locus_tag="RHW33_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV1644738.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            complement(20552..21604)
                     /gene="ompA"
                     /locus_tag="RHW33_001214"
     CDS             complement(20552..21604)
                     /gene="ompA"
                     /locus_tag="RHW33_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV1644739.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(21961..22470)
                     /gene="sulA"
                     /locus_tag="RHW33_001215"
     CDS             complement(21961..22470)
                     /gene="sulA"
                     /locus_tag="RHW33_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV1644740.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLPPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22689..23318
                     /gene="sxy"
                     /locus_tag="RHW33_001216"
     CDS             22689..23318
                     /gene="sxy"
                     /locus_tag="RHW33_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV1644741.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23281..25443)
                     /gene="yccS"
                     /locus_tag="RHW33_001217"
     CDS             complement(23281..25443)
                     /gene="yccS"
                     /locus_tag="RHW33_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV1644742.1"
                     /translation="MAYMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25453..25899)
                     /locus_tag="RHW33_001218"
     CDS             complement(25453..25899)
                     /locus_tag="RHW33_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV1644743.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26022..28076
                     /gene="helD"
                     /locus_tag="RHW33_001219"
     CDS             26022..28076
                     /gene="helD"
                     /locus_tag="RHW33_001219"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV1644744.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWGKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28108..28566)
                     /gene="mgsA"
                     /locus_tag="RHW33_001220"
     CDS             complement(28108..28566)
                     /gene="mgsA"
                     /locus_tag="RHW33_001220"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV1644745.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28662..29324)
                     /locus_tag="RHW33_001221"
     CDS             complement(28662..29324)
                     /locus_tag="RHW33_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV1644746.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29497..29910
                     /locus_tag="RHW33_001222"
     CDS             29497..29910
                     /locus_tag="RHW33_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV1644747.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29955..30272)
                     /gene="hspQ"
                     /locus_tag="RHW33_001223"
     CDS             complement(29955..30272)
                     /gene="hspQ"
                     /locus_tag="RHW33_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV1644748.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30330..31520)
                     /gene="rlmI"
                     /locus_tag="RHW33_001224"
     CDS             complement(30330..31520)
                     /gene="rlmI"
                     /locus_tag="RHW33_001224"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV1644749.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31615..31893
                     /gene="yccX"
                     /locus_tag="RHW33_001225"
     CDS             31615..31893
                     /gene="yccX"
                     /locus_tag="RHW33_001225"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV1644750.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31890..32219)
                     /gene="tusE"
                     /locus_tag="RHW33_001226"
     CDS             complement(31890..32219)
                     /gene="tusE"
                     /locus_tag="RHW33_001226"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV1644751.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32310..32969)
                     /gene="yccA"
                     /locus_tag="RHW33_001227"
     CDS             complement(32310..32969)
                     /gene="yccA"
                     /locus_tag="RHW33_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV1644752.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33176..33263)
                     /locus_tag="RHW33_001228"
     tRNA            complement(33176..33263)
                     /locus_tag="RHW33_001228"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33227..33229),aa:Ser,seq:tga)
     gene            33689..34807
                     /gene="hyaA"
                     /locus_tag="RHW33_001229"
     CDS             33689..34807
                     /gene="hyaA"
                     /locus_tag="RHW33_001229"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV1644753.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34804..36597
                     /gene="hyaB"
                     /locus_tag="RHW33_001230"
     CDS             34804..36597
                     /gene="hyaB"
                     /locus_tag="RHW33_001230"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV1644754.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDATTVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPTTWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            36616..37323
                     /gene="hyaC"
                     /locus_tag="RHW33_001231"
     CDS             36616..37323
                     /gene="hyaC"
                     /locus_tag="RHW33_001231"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV1644755.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37320..37907
                     /locus_tag="RHW33_001232"
     CDS             37320..37907
                     /locus_tag="RHW33_001232"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV1644756.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            37904..38302
                     /gene="hyaE"
                     /locus_tag="RHW33_001233"
     CDS             37904..38302
                     /gene="hyaE"
                     /locus_tag="RHW33_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV1644757.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38299..39156
                     /locus_tag="RHW33_001234"
     CDS             38299..39156
                     /locus_tag="RHW33_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV1644758.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39290..40834
                     /gene="appC"
                     /locus_tag="RHW33_001235"
     CDS             39290..40834
                     /gene="appC"
                     /locus_tag="RHW33_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV1644759.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQSFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40846..41982
                     /gene="appB"
                     /locus_tag="RHW33_001236"
     CDS             40846..41982
                     /gene="appB"
                     /locus_tag="RHW33_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV1644760.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGN
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41995..42087
                     /gene="cbdX"
                     /locus_tag="RHW33_001237"
     CDS             41995..42087
                     /gene="cbdX"
                     /locus_tag="RHW33_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV1644761.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42167..43471
                     /gene="appA"
                     /locus_tag="RHW33_001238"
     CDS             42167..43471
                     /gene="appA"
                     /locus_tag="RHW33_001238"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV1644762.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVR
                     APTKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCP
                     QPSQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLD
                     NANVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQAL
                     PSELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNA
                     QFYLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANL
                     GGALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSL
                     NTPPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43591..45771)
                     /gene="etk"
                     /locus_tag="RHW33_001239"
     CDS             complement(43591..45771)
                     /gene="etk"
                     /locus_tag="RHW33_001239"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV1644763.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(45791..46237)
                     /gene="etp"
                     /locus_tag="RHW33_001240"
                     /gene_synonym="yccY"
     CDS             complement(45791..46237)
                     /gene="etp"
                     /locus_tag="RHW33_001240"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV1644764.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46225..47364)
                     /locus_tag="RHW33_001241"
     CDS             complement(46225..47364)
                     /locus_tag="RHW33_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV1644765.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(47410..49506)
                     /locus_tag="RHW33_001242"
     CDS             complement(47410..49506)
                     /locus_tag="RHW33_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV1644766.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQMGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(49506..50252)
                     /locus_tag="RHW33_001243"
     CDS             complement(49506..50252)
                     /locus_tag="RHW33_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV1644767.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAVKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50249..50893)
                     /locus_tag="RHW33_001244"
     CDS             complement(50249..50893)
                     /locus_tag="RHW33_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV1644768.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLHIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(51000..51305)
                     /locus_tag="RHW33_001245"
     CDS             complement(51000..51305)
                     /locus_tag="RHW33_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644769.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
BASE COUNT        12745 a        12707 c        13541 g        12482 t
ORIGIN      
        1 tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg gaattgtgac
       61 cgtaagttta acggcgatat tactcagctg ctcatccgca tgggcggcag gtaaaggcgg
      121 gattggactt gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa tttcactggg
      181 tgtacgtaac accagtccgg atgttcctta tcttattcag tcatgggtga tgaccccaga
      241 taataaaaaa tcagcagact ttattattac accaccgcta tttgtgctaa atccggctaa
      301 tgagaatctg ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca gagaaactct
      361 tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagaag gcaataccct
      421 gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt tagcgtatcc
      481 cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg ttacggtcag
      541 taacccaaca ccttatttca ttaccctgac agacctgaaa atagatggaa aggtagttaa
      601 aaatcaaatg atttccccct ttgataaata ccaattctct ctgccaaagg gtgccaaaaa
      661 tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc aactcaactg
      721 taaatcgtaa ttcgtcttca gttaagagag cgagatgtat agaactcacc gacaacacag
      781 cctgttaagc tctggtggcg tgccatcgtt tattggtggg ctggtggtgt ttgtgtcggc
      841 agcgttcaat gcacaagctg aaacctggtt cgatccagcc tttttcaaag atgatccctc
      901 aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag gggtttatcg
      961 tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt ttgttgagat
     1021 aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg caatgggcgt
     1081 gaatactgat gcatttccgg cttttaaaca actggacaaa caagcgtgtg cgctattggc
     1141 ggagattatt ccggatgcca gcgtaacttt taatgtgaat aaactccgtc tggaaatttc
     1201 agtaccgcaa attgccatca aaagtaatgc tcgtggttat gtcccccctg aacgttggga
     1261 tgaagggatc aacgcgctat tactgggata ttcatttagc ggggctaaca gtattcatag
     1321 cagcgcagac agtgattctg gcgacagtta ttttctgaat ttaaacagtg gcgttaattt
     1381 aggcccatgg agattgcgca acaattcaac atggagtcgt agtagtggcc aaaccgcaga
     1441 atggaagaat ctcagcagct atttgcaacg ggcggttatt cctctgaaag gcgaactgac
     1501 tgtaggtgat gattatactg caggcgattt tttcgatagt gtcagctttc gtggtgtgca
     1561 gctggcgtca gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc ctgtggtgcg
     1621 tggtatcgcc aaaagcaatg cccagataac gattaagcaa aatggttaca ccatttacca
     1681 aacttatgta tcgcctggtg cttttgaaat tagtgatctc tattccacgt cgtcgagcgg
     1741 tgatttgtta gttgaaatca aagaagcgga cggcagcgtc aatagctaca gcgtaccctt
     1801 ttccagcgtg ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga cactggcgaa
     1861 atacagaacc aatagtaatg aacagcagga gagcaaattt gcccaggcca cgttgcagtg
     1921 gggcggaccg tggggaacga cttggtatgg tggtggacaa tatgctgaat attaccgtgc
     1981 cgccatgttt ggtctgggat ttaacctcgg cgatttcgga gcaatttcgt tcgatgtgac
     2041 ccaggcgaag agtacgctgg cagaccaaag cgaacataaa ggtcagtcat atcgttttct
     2101 gtatgccaaa acgctcaacc aattgggtac taactttcaa ttgatgggct atcgctattc
     2161 gacgtcgggt ttctacaccc tttccgacac catgtataaa catatggatg gctacgaatt
     2221 taatgacggt gatgatgaag atacgccgat gtggtcgcgt tattacaatt tgttttacac
     2281 caaacgtggc aaactgcagg tcaatatctc ccagcaatta ggcgagtacg gttcgtttta
     2341 tttaagtggt agccagcaaa cttactggca taccgatcaa caggagcggc tattacagtt
     2401 tggctacaac acgcaaatta aagatctctc gctaggggtt tcctggaatt acagtaagtc
     2461 ccgtggtcaa cctgatgctg atcaggtgtt tgcacttaat ttttccctgc cgatcaatct
     2521 gttgctcccc agaagtaatg atagctatac cagcaaaaaa aattacgcct ggatgacctc
     2581 taacaccagt atcgataacg aagggcacac tacacaaaac ctgggtttaa cggagacact
     2641 actcgatgac ggtaacctga gctacagcgt gcaacaggga tataacagcg aggggaaaac
     2701 ggctaatggt agcgccagca tggactacaa aggggcgttt gcggatgccc gggtgggcta
     2761 caactacagc gataacggca gtcaacaaca actgaactac gctatttcag gcagtttagt
     2821 tgcccattca cagggcatta ccctggggca atcgctgggg gaaactaacg ttctgattgc
     2881 agcgccaggc gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa ctgactggcg
     2941 cggatatact gttgttcctt atgccacttc ttatcgggaa aatcgaatcg cacttgatgc
     3001 ggcatcgtta aaacgtaacg tggatcttga aaatgcagta gttaacgtgg ttcccaccaa
     3061 aggggcgttg gttctggcgg agttcaatgc ccatgcgggt gcaagggtat taatgaaaac
     3121 atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg acgctggacg gcgtacagac
     3181 taatagcggc ataattgatg atgatggctc gctctatatg gcgggtttac cggcgaaggg
     3241 aacaataacc gtgcgctggg gcgaagcttc cgatcaaatt tgtcatatca gttacgagct
     3301 taccgaacaa caaattaact ctgcgattac gcgaatggat gccatatgca gataatcttt
     3361 ggagaaaaat gcgtgtcatt actacgacta ttttttgccg ccgtcttaat gctatggtgc
     3421 gctcaaaccg ctgcttatag cgggcagtgt cataccactc aggggaatcc gtatattggc
     3481 gtcaattttg gcgttaaaac cctggaggaa gaagaaaata cgactggggt agttaaagac
     3541 aaattttatc agtggaacga atcgaatgat tattatgttt cctgtgattg cgataaagac
     3601 aatgtcagaa gtggccgatg ggcattcgcc gcggattcac cgttagtcta tttaggcgac
     3661 aactggtaca aaattaatga ctatcttgcc gccaaagttt tattgcaggt taaaggcagt
     3721 tctcctacag cggttccttt cgaaaacgtg ggcacagggg gggatacccg atggcatatt
     3781 tgtgaccccg gcggtcaacg tttaggcggc cagggggcta gcggtaatag cggtagcttt
     3841 tccctgaaaa tattgcagcc gttcgttggt tcggtcgtca ttccacctat ggcgctggcg
     3901 cgattatttg aatgctacaa catacccgca ggtgattcct gcacgactac aggtacaccg
     3961 gttttagtgt attacctgtc tggtactatc aattcacttg gctcatgttc cgtcaatgcc
     4021 ggagaaacaa tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg tgttgtaggg
     4081 cataagcctc ttggggccag aacggcagaa cttgcaattc cagtcaggtg taacacggga
     4141 aacgcggggt tagttaacgt caacctgagt ctgacggcaa ccacagaccc cagctatccc
     4201 caggcgatta agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag ccagaacaac
     4261 attatttccc ctgctggtgg aacactaccg ctctctattc ctgatgatgc agacagtatc
     4321 gcgcgaatga atgtctatcc agtcagcacg acaggtgtac caccagaaac cgggcgattt
     4381 gaagccacgg caacggtgag aataaatttt gattaacgcg tgaatgtatg aagaaaaaaa
     4441 cgatatttca gtgcgttatc ttgttcttta gccttcttaa catccatatc gggatggctg
     4501 ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc tgtgatgtcg
     4561 ctaccaaaag tgcattacaa aatattcata ttggcgattt taatatcagt gattttcagg
     4621 ccgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt tgtgccgctg
     4681 gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgca ccgacactgc
     4741 tcaaactaac tgacacaggc ggaagcggtg gtatggcaac agggattgcc gtgcaaattc
     4801 ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt acgcccttaa
     4861 aagccgggga taacacactc aaatatcaac ttcgttataa gtccacaaag gcgggagcaa
     4921 cgggcggtaa tgcgacggcg gttctctatt ttgatctggt ttaccagtga aggagtgatg
     4981 ttgaaacgga taatctggat tctgttctta ctgggattaa cggggggttg tgagctattt
     5041 gcccatgatg gcacggtcaa cattagcgga tcgtttcgcc gtaatacatg cgtgctggca
     5101 caggatagca agcaaatcaa tgtgcagtta ggcgatgtct cactgacacg tttttctcat
     5161 gggaattatg gcccggagaa atctttcatc attaatttgc aggattgcgg aacagatgtc
     5221 agcacggttg acgtcacctt ttcaggaacc tccgacggag tgcagagcga aatgttgtcc
     5281 attgaaagtg gtacggatgc agccagcggg ctggcaattg cgattctgga tgatgcgaag
     5341 atattgattc cgcttaacca ggcgagtaaa gactacagcc tacatagcgg taaggtgcca
     5401 ttaacttttt atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg taaagtgaat
     5461 gccagcgcga catttgtact tcattatgac taatacgtgg aacagattgg cgctcttgat
     5521 tttcgccgtt ttatcgctgc tggtggcggg tgaattgcag gcaggagtgg tggtcggcgg
     5581 aacgcgattt atctttccgg cagacagaga atcgatatct attttactga ctaataccag
     5641 tcaggaatcc tggcttatta acagtaaaat caaccgccca acgcgttggg ccgggggtga
     5701 agcgtcgaca gtgccagcac cattactggc cgctccgcca cttattctcc tgaagcccgg
     5761 tacgacaggc acgttgcgct tgctgagaac ggaaagcgac atcttgcctg tggatcgcga
     5821 aacgctattt gagttaagca ttgccagcgt gccatccggc aaagttgaaa atcagagcgt
     5881 aaaagtggcg atgcgctcgg tatttaaact gttctggcga cccgaaggtt tgccgggtga
     5941 cccgctggaa gcttatcaac aattacgctg gacacggaat tcgcagggtg tacaactcac
     6001 taacccaacg ccttactaca ttaacctgat tcaggtgagc gtaaatggta aagcgttaag
     6061 taatgtggga gttgtgccgc ctaaaagcct gcgtcagaca agctggtgtc aggcaatagc
     6121 accgtgtcat gttgcctggc gggcgataaa tgattacggc gggttgagtg caaagaagga
     6181 gcaaaatctg ccctgaaaca ggttcggaaa acgtttgcgt tttttttgcc gcaggtcaat
     6241 tcccttttgg tccgaactcg cacataatac gcccccggtt tgcacaccgg gaatccagga
     6301 gagttcatgt actacccctt cgttcgtaaa gcccttttcc agctcgatcc agagcgcgct
     6361 catgagttta cttttcagca attacgccgc attaccggaa caccgtttga agcactggtg
     6421 cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa aaatccgctt
     6481 ggtctggcag ccggtcttga taaagacggg gagtgcattg acgcgttagg cgcgatggga
     6541 tttggatcga tcgagatcgg taccgtcacg ccacgtccac agccaggtaa tgacaagccg
     6601 cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt taataatctt
     6661 ggcgttgata acctcgtaga gaacgtaaaa aaagcccatt atgacggcgt cctgggtatt
     6721 aacatcggca aaaataaaga tacgccagta gagcagggca aagatgacta tctgatttgt
     6781 atggaaaaaa tctatgccta tgcgggatat atcgctatca atatttcatc gccaaatacc
     6841 ccaggattac gcacactgca atatggtgaa gcgctggatg atctcttaac tgcgattaaa
     6901 aataaacaaa atgatttgca agcgatgcac cataaatatg tgccgatcgc agtgaagatc
     6961 gcgccggatc tttctgaaga agaattgatc caggttgccg atagtttagt tcgccataat
     7021 attgatggcg tgattgccac caataccaca ctcgatcgtt ctctggttca gggaatgaaa
     7081 aattgcgatc aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag caccgaaatt
     7141 attcgccgct tgtcactgga attaaacggt cgcttaccga tcatcggtgt tggcggcatc
     7201 gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt gcaaatttat
     7261 tctggtttta tttttaaagg tccgccgctg attaaagaaa tcgttaccca tatctaatta
     7321 tttcttcgaa ttcctcttta taagataatc agggctttat tttcagccct ggttgtttta
     7381 tattcatcac tgttgcttat ttagacattt tgtactttta ttgttgaggt tattaagcga
     7441 agcgacaatg gattgtgttg ctgcggtttt ataaggttgg ggagaggcag atgcgaatta
     7501 aaccagacga taactggcgt tggtattacg atgaagagca cgatcgaatg atgctcgatt
     7561 tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa aatgttgact ccagatgcct
     7621 tttctcccgc aggcttttgc gttgacgacg ccgcgctcta tttctctttt gaagaaaagt
     7681 gccgcgattt taatttatct aaagaacaaa aagccgaact ggtgctgaat gcactggtag
     7741 caattcgcta tctcaaaccg caaatgccga aaagctggca ttttgtttcc catggtgaaa
     7801 tgtgggtgcc catgccgggg gatgcggcct gcgtttggtt gagcgacact aacgagcaag
     7861 tgaatttact ggtcgttgaa tctggcgaga atgccgcgct atgcctgctg gcacaaccct
     7921 gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc atgaacgaca
     7981 ggctgaaacc gcaggttaat gttgacagct tcagcctcga acaggcagtc taacgcgcca
     8041 acttaagtgc agtcttcggt acacagctac agcaaagaat ggtgccatca tcgcccattg
     8101 ctgatttttt cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa cttccacaaa
     8161 tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt ttctaattgc tccagcaaca
     8221 cctgttggtt atttccacga aatgcctgtc cctgccagtc aatatctaca ttggcgtccg
     8281 gttgttgcgt gatgttggca gtatcatcag cggcagctgc gccgtaaatt tttgccggag
     8341 ccgttgtcag aatttccacc tcatcgccaa cgcgaatcac gccgctatta cgggcaatta
     8401 aattctgacc aaaatcgaca tcgccgttat cctgggcagt gcggaaagat tgcaatgttt
     8461 ttaatggttc gcctgccgga tgtttttgcc ctttttctgg gctgacggtg gtgaaaatac
     8521 agcggctaca aggtttaacc acatcaaaca ccacatcacc aatgcgaatc actttccaac
     8581 tatcttcttc ccaggctgac gcgccggaaa ccaccagatt ggggcggaat tgctccattt
     8641 ttacactggc cggacaacgt tgttggagat cacgtaacga ggcttcgtta gcaagaaggt
     8701 aaggatagcc atcagcaaat gacaggggta cagtgttgtg gcgtttcacg cgccgggtca
     8761 tttgtggccc cacccagcgt aattgcactt cgcgggagaa aaatccactt aaccatttgt
     8821 tgatcgcttc tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg gttggtgcgt
     8881 cttgtgtggc gaaatcagca aaacgaacat atgcgctact gccatctggt gcggtgagat
     8941 gcaagccatc atgcacaggt gaaggagtaa accgtaccat ctggggaaac tggcgagcgg
     9001 taataaacgt accgtcaggt tccgtgatca taaagatgcg atcgaaggcc agaccactga
     9061 catcagccag agcatgtgta agaccaatgc cgcgcatcga tttaacagga tgaataaaaa
     9121 gccgggttaa tgtcgccacg gtgtggtcct caaatgaaaa taagccctca actttatgac
     9181 atgacgcgct tattagctat aatgcgcaac aattttctta gcgcctgtcc cattaggcta
     9241 ttttatttgt cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt gtgtacgctc
     9301 cggtttctcc gcgctgtcca tgtccagact gactgcaaca attacgccta ctgcggtagg
     9361 ctcttagagt aaaagtgacg atatgaattc tctgtttgcc agtacggccc gtgggctgga
     9421 agagttgtta aaaactgaac tggaaaacct gggggccgtt gaatgccagg tggttcaggg
     9481 tggggtccat ttcaagggcg acacacggct tgtttaccag agcctgatgt ggagccgcct
     9541 ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt tacagcgatt tagacctcta
     9601 tctcggtgtt caggcgatca actggacaga gatgtttaat cctggcgcga ccttcgctgt
     9661 ccacttcagt ggtttgaatg acacaatccg caacagtcag tacggcgcga tgaaagtgaa
     9721 agacgcaatc gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg ttgatcgcga
     9781 tgcgccggat atccgcgtta acgtctggct gcataaagaa accgccagta tcgctcttga
     9841 tctgagtggt gatggtttac atctgcgtgg ctatcgcgat cgtgctggta ttgcgccgat
     9901 caaagaaacc ctggcagccg ctattgtgat gcgatccggc tggcagccag gaacaccgct
     9961 gctcgatccg atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc tggcgaccga
    10021 tcgcgcacca ggcttgcacc gtgggcgttg gggctttagc ggctgggcgc agcatgatga
    10081 agctatctgg caggaagtga aagcagaagc gcaaactcgc gcccgtaaag gcctggctga
    10141 gtatagctct catttttacg gttcggacag cgacgcacgg gtgattcaac gtgcgcgcac
    10201 taacgcccgt cttgcgggga ttggtgaact gatcaccttt gaggtgaaag atgtcgcgca
    10261 actgaccaat ccgctgccga aagggccgta cggcacagtg ttgagcaacc cgccatacgg
    10321 ggaacgtctg gacagcgaac cggcgctgat tgcgctgcat agcctgctgg gtcggatcat
    10381 gaaaaaccag tttggtggct ggaatctctc tttgtttagt gcctcgccgg atctgctaag
    10441 ctgcttgcag ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc tggactgcgt
    10501 acagaaaaat taccacgttg ccgaaagcac tccagacagc aaaccggcga tggtagcgga
    10561 agattacgcc aaccgcctgc gtaagaacct caaaaaattc gagaagtggg ctcgccagga
    10621 aggaattgaa tgttaccgcc tgtatgacgc cgatctgccg gaatataacg ttgccgttga
    10681 ccgttatgcc gactgggtgg tggtgcagga gtatgcaccg ccaaaaacta ttgatgcgca
    10741 caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc atttcggtac tggggattgc
    10801 gccaaacaaa ctggtgctga aaacccgtga acgccagaag ggcaaaaacc agtaccagaa
    10861 actgggcgag aagggcgagt ttcttgaagt taccgaatat aacgctcact tgtgggtgaa
    10921 cctgacggat tatctcgata ctggtctgtt cctcgatcac cgtatcgccc gccgtatgct
    10981 cggtcagatg agcaaaggca aagatttcct caacctgttt tcttataccg gcagcgccac
    11041 cgtgcatgcg ggattaggcg gtgcacgcag caccaccacc gtggatatgt cgcgtactta
    11101 tctggaatgg gcagaacgca acctgcgtct gaatggcctg accgggcgtg cgcatcgcct
    11161 gattcaggcc gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg atctgatctt
    11221 tatcgatccg ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg atgttcagcg
    11281 cgatcatctg gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg ggacgatcat
    11341 gttctcgaac aacaaacgtg gcttccgtat ggatctcgac ggcctggcaa aactgggact
    11401 gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta accgccagat
    11461 ccacaactgc tggctgatta ccgcagcctg aaaggaatag taatgtcatt aatcagtatg
    11521 catggcgcat ggctgtcgtt cagcgacgcg ccgcttctcg ataacgcaga actgcatatc
    11581 gaagataacg aacgtgtttg tctggtgggc cgcaacggcg caggcaaatc gacgttaatg
    11641 aaaatcctca accgtgaaca agggctggat gacggtcgca ttatttacga gcaggatttg
    11701 attgtagcgc gtctgcaaca ggatccgccg cgtaacgttg agggtagtgt ttatgatttc
    11761 gttgccgaag gcattgaaga acaagcggaa tacctgaaac gctatcacga tatttcgcgc
    11821 ctggtgatga acgacccgag cgagaaaaat ctcaacgaac tggcaaaggt tcaggaacag
    11881 ctggatcacc acaacctgtg gcagctggaa aaccgcatca acgaagtgct ggcgcaactg
    11941 gggttagatc ctaacgttgc gctgtcgtcg ctttccggcg gctggttgcg taaagcggca
    12001 ttaggacgcg cgctggtgag taatccgcgc gtattgctgc tggacgaacc gactaaccat
    12061 ctggatatcg aaaccatcga ctggctggag gggttcctta aaaccttcaa tgggacgatt
    12121 attttcatct cccacgaccg ttcgtttatc cgcaatatgg cgacgcgcat tgttgatctc
    12181 gatcgcggca agctggtgac ctatccgggc aattacgatc agtacctgct ggaaaaagaa
    12241 gaagccctgc gcgtggaaga actacaaaat gccgagttcg atcgcaaact ggcgcaggaa
    12301 gaagtgtgga ttcgccaggg gatcaaagca cgccgtaccc gtaatgaagg ccgcgtacgc
    12361 gccctgaaag cgatgcgtcg cgagcgtggc gaacgtcgcg aagtgatggg taccgcaaag
    12421 atgcaggtgg aagaggccag ccgctccggt aagatcgttt tcgaaatgga agacgtttgc
    12481 taccaggttg acggaaagca actggtgaaa gatttttctg cccaggttct acgtggcgac
    12541 aaaattgccc tgattgggcc gaatggctgc ggcaaaacca cgctgctgaa actgatgctc
    12601 ggtcagcttc aagcggacag cgggcgtatt cacgttggca ccaaactgga agtggcttat
    12661 ttcgatcagc accgcgcgga acttgatccc gataaaacgg tgatggataa ccttgccgaa
    12721 ggtaagcaag aggtgatggt taacggcaag ccacgccacg ttttgggcta tttgcaggac
    12781 ttcctgttcc atccgaaacg ggcgatgacg ccagtacgtg cgctttctgg cggtgagcgg
    12841 aaccgcttgc tgctggcgcg tttgttcctc aaaccaagca acttattgat tcttgacgaa
    12901 ccgaccaacg atcttgatgt cgaaacgctg gagctgctgg aagaactgat cgacagctac
    12961 cagggcacgg tattgctggt aagccacgat cgtcagtttg ttgataacac cgttacagaa
    13021 tgctggatct tcgaaggcgg cggtaaaatt ggtcgttatg tcggcggtta tcatgacgcc
    13081 cgtggtcagc aagagcagta tgtggcgctc aaacagcctg cggtgaaaaa aaacgaagaa
    13141 gccgccgcgc caaaagcaga aactgtaaaa cgcagcagta gcaaactaag ctataaattg
    13201 cagcgcgaac tggagcagct accgcaattg ctcgaagatc tggaggcgaa gctggaagcc
    13261 ctacagacgc aggtggcgga tgcttccttc ttcagtcagc cgcatgagca gacacaaaaa
    13321 gtgctcgctg atatggctgc tgcagagcag gagctggagc aagcctttga acgctgggag
    13381 tatcttgaag cgttaaaaaa tggtggctga tcgcaaaata gtcgatttaa acagagacag
    13441 ctgacaacgg taaaattgcc tgatgaactg ggtttatcag gcatgaaaac caacactttg
    13501 ttagtcatct gtaaaatagc gcatcattaa ggagtaccaa tgtgcgaaca tcatcatgcc
    13561 gcgaagcaca tcctatgctc gcagtgtgac atgctggtgg cgttaccgcg ccttgagcat
    13621 ggtcagaaag cggcatgtcc ccggtgtggc acaacgttaa ccgtggcgtg ggatgccccc
    13681 cggcagcgtc ctaccgccta tgcgttggct gcactgttca tgctgttgct gtccaacttg
    13741 tttccttttg tgaatatgaa cgttgcagga gttaccagtg aaattacatt actggaaatt
    13801 cccggcgtgc ttttttctga ggactacgcc agcctcggca cctttttcct gttgtttgtg
    13861 caactggttc ccgcgttttg tctgataacc attctgttac tggtgaatcg cgcggaatta
    13921 ccggtccgtt taaaagagca actggcacgg gtgctttttc aactcaaaac ctggggaatg
    13981 gcggagattt tcctcgcggg tgtgctggtc agtttcgtta aactgatggc ttacggcagc
    14041 attggcgtag gcagcagctt tctcccctgg tgtttatttt gtgtcctgca actgcgcgct
    14101 tttcagtgcg ttgatcgtcg ctggttatgg gacgatatcg ccccgatgcc agaactgcgc
    14161 cagccgctaa aaccaggcgt cacggggata cgtcaggggc tgcgttcgtg ctcctgttgt
    14221 acggcaatcc ttcctgctga cgaacccgtg tgcccgcgtt gtagtaccaa agggtacgtt
    14281 cgacgtagaa acagcctgca atggacactc gcgctgcttg ttacgtccat catgctgtat
    14341 cttccggcta atattttgcc catcatggtg acggatttat taggctcgaa gatgccatcg
    14401 acgattctcg ctggggtcat cctgttatgg agcgaaggct cttatcccgt cgctgcggtg
    14461 atcttccttg ccagtattat ggtgccaacg ttaaagatga tcgccatcgc gtggctgtgt
    14521 tgggatgcca aagggcatgg caagcgcgac agtgaaagaa tgcatttgat ttatgaagtt
    14581 gttgagtttg taggccgctg gtcgatgatt gacgttttcg ttatcgcggt gctctcggcg
    14641 ctggtgcgta tgggaggttt aatgagtatt tatccggcaa tgggtgcatt aatgtttgct
    14701 ttagtcgtca taatgacaat gttttctgct atgacgtttg acccgcgttt gtcgtgggat
    14761 cgtcaacctg aatcagagca tgaggagtcc tgacagtatg gaatctaata atggggaagc
    14821 caaaatccag aaagtgaaga actggtctcc cgtgtggata tttcctatcg tcacggcgct
    14881 cattggggcc tgggttcttt tttatcatta cagccatcag ggaccggaag tgaccctgat
    14941 caccgcgaat gcggaaggaa ttgaaggtgg caaaaccacc attaaaagcc gtagcgttga
    15001 cgtcggcgtg gttgaaagcg ccacactcgc tgatgatttg acacacgttg aaatcaaagc
    15061 gcggctgaat tccggtatgg aaaaattgct gcataaagat accgtctttt gggtggtgaa
    15121 accgcagatt ggtcgagaag ggattagcgg tctgggaacc ctgctgtctg gggtttatat
    15181 cgaactgcag ccaggcgcga aaggcagcaa aatggataaa tacgatttgc tggactcgcc
    15241 accgttggcc ccgcctgatg cgaaaggtat ccgtgtgatt ctcgatagca aaaaagccgg
    15301 gcagctctcg ccaggagatc cggtgctgtt ccgtggctat cgggtaggtt cggttgaaac
    15361 cagcaccttc gatacgcaaa aacgcaatat cagttatcaa ctgttcatca atgcacctta
    15421 tgaccgactg gtgaccagca atgttcgctt ctggaaagat agtggcattg cggttgatct
    15481 gacgtcagcg ggaatgcgtg tggagatggg ctcattgaca acgctgctga gtggcggtgt
    15541 cagctttgat gtgccggaag gtctggattt agggcagcca gtggcaccga aaacagcttt
    15601 cgttttgtat gatgatcaga agagcattca ggattcgttg tacaccgatc acattgatta
    15661 tctgatgttc tttaaagatt cggtacgcgg tctgcaaccg ggagctccgg tagaattccg
    15721 gggtattcgc ctgggtaccg taagcaaagt gccattcttt gcgccgaata tgcgtcagac
    15781 atttaacgat gattaccgta ttccggtgct gattcgtatc gagccagagc ggctgaaaat
    15841 gcagcttggc gaaaatgcgg atgttgttga gcaccttggc gaattgttga aacgtggttt
    15901 acgcggatcg ctgaaaaccg gaaacctggt cactggcgca ctgtatgttg atctcgattt
    15961 ctatccaaat acgcctgcaa taaccggtat tcgtgaattt aatggttatc agattatccc
    16021 gaccgttagc ggcggcctgg cgcaaatcca gcaacgactg atggaagcgt tggataagat
    16081 caacaaactg ccattgaatc cgatgattga acaggcaacc agtacgcttt ctgaaagtca
    16141 gcgcacaatg aaaaacctgc aaacgacgct ggatagcatg aacaagatcc tcgccagcca
    16201 gtcgatgcag cagttgccga cggatatgca gtcaacgttg cgtgaattga atcgcagcat
    16261 gcagggcttc cagcctggct ccgcagccta caacaagatg gtggcggata tgcagcgcct
    16321 tgatcaggtg ttgcgagaac tgcaaccggt gctgaaaacg ctcaatgaga agagtaatgc
    16381 gctggtattt gaagcgaagg acaaaaaaga tccagagccg aagagggcga aacaatgaaa
    16441 aagtggctag tgacgattgc agcactgtgg ctggccggat gcagctccgg cgaaattaat
    16501 aaaaactatt accagttacc tgtggtgcag agcggtacac aaagtaccgc cagccagggc
    16561 aatcgcctgt tatgggtaga gcaggtcgct gtacctgact atctggcggg gaatggtgtg
    16621 gtttatcaaa ccagtgatgt gaagtatgtg attgccaaca acaacttgtg ggccagcccg
    16681 ttggatcaac agttgcgcaa caccctggta gccaacctga gcacgcaact gcccggctgg
    16741 gtggttgcct cccagcctct gggaagcgcc caggacacgc tcaatgttac cgtaacggag
    16801 tttaacggtc gctatgatgg caaggtcatt gtcagtggtg agtggctgtt gaaccaccag
    16861 ggacaactga tcaaacgtcc gttccgtctg gaaggggtgc aaacgcagga tggttacgat
    16921 gagatggtta aagtgctggc cagtgtctgg agtcaggaag ccgcttctat tgcacaagag
    16981 ataaagcgtc taccttaatt ataaagattt gtaaatataa ccgtctccgg tatgtcgcct
    17041 gaggcggttt ttttgtttct aacgtacgga aaaatttgtt cctcttcaca ttttttgtac
    17101 aaccgacatg cccgtgtagc tcacaaatat gacagtggcg tgaattttgc gcattgacgg
    17161 cagttatgat tcgcggtatt gcttaactgt gattgcacat ttagtaatca ctgttttctt
    17221 ttccaccaga aaccagtatg agggaaacga ggcatgaaga gacaaaaacg agatcgcctg
    17281 gaacgggcac atcaacgtgg ttatcaggcc ggcatcgccg gacgctcaaa agaaatgtgt
    17341 ccctatcaga cgctgaatca aaggtcacaa tggctgggag gctggcgaga agccatggcg
    17401 gacagggtag taatggcctg attctgtctc tttaaaaaga aacctccgca ttgcggaggt
    17461 ttcgcctttt gatactctgt ctgatgataa tcagaaggca gacgtatcct ggaacagacc
    17521 gactttcagg tcgctggcgg tatagatcag acgaccatca accagcactt cgccatctgc
    17581 cagacccata atcagacgac gattaacaat gcgtttaaag tgaatacggt aggtcacttt
    17641 tttcgctgtc ggcagtacct gaccagtgaa tttcacttcg ccaacgccca gcgcgcggcc
    17701 tttaccttcg ccgcccagcc agccgaggta gaaccctacc agctgccaca ttgcgtccag
    17761 gcccaggcat cccggcataa ccggatcgcc aataaagtgg catccgaaga accacagatc
    17821 cggattgata tccagttctg cttcaacata ccctttgtcg aagttaccac ccgtttcggt
    17881 cattttgacc acacggtcca tcatcagcat gttcggtgct ggcaattgcg ggcctttagc
    17941 gccaaacagt tcaccgcgac cagaggcaag aaggtcttct tttgtatagg attcgcgttt
    18001 atctaccatg ttctctgtaa gccttatttt attgaagcac gcaggatagc taacacgtgt
    18061 acgctgaaca agtccgatca gttcggaata aaccagttca gccaacgtaa tggccatgga
    18121 aaacggtgac gtccttcctg ttgcgatgct tgcgcgatac gttcctggat ggtttgcatc
    18181 agcgtcgttt ggccttcgcc atcccacacc agatttaata ataacggcag tgcgtcagtc
    18241 acatcgtcta ctgcccaaat ggtgaatttg ccttcttcta ccgctttcac cagttcactg
    18301 tgaagactta aatggcgaac gttagcagtg gggataatga caccttgttt ccccgttaac
    18361 tcacgttgct ggcaaatagc aaagaagcct tcgattttct catttaaacc accgaccggc
    18421 tggacgcggc cgaactgatc gactgaacct gtgatagcga tactctgatt caccggcata
    18481 tcagcgaggg cgcttatcag ggcgcagagt tcagccatcg aggcactatc tccatccact
    18541 tcactgtatg actgctcaaa tgtcagcgat gctgagaagg ggatctgttg ctcaagctgt
    18601 agttccgaca tcaggaacgc ctgcatgatc atcatccctt tcgcatggat attgccgcca
    18661 agctccgctt tgcgttcgat gtcggtgaat tcaccatcgc caatatgcac aacgcagcta
    18721 atgcgagaag gttcgccaaa agcgcgtgga tgacccggaa attcaatgac cgaaagggcg
    18781 ttaatttgcc cgatgcgttc gccttcggtt tcaatcagga tttgctcctg aaggatctca
    18841 tcctgcatgc gttcagcgag gaaaccttcg cgccattcac gctgctgtag cattaagttt
    18901 agctgctcgc cggagaaggt gtcgccatca cacagggagg cgacttcttt acactggcgg
    18961 aggatccact gcgggctaag cggaagtgtt tcttgttcac cggtgtagcg tgctgcttcg
    19021 cggataagta tcggccaggc atccgctccc ggtgcaggta agtgattatg tctggcggta
    19081 aatgtcaccc agcgacacca ctggcttact gactccgcat cgacaatctg gagagtatct
    19141 tcaaattcgc tataaatagc ctgctctgaa agctctggct ccatctcctg gaaatcagcc
    19201 aatgattcgc gttcgcctac cagaatgact ttcagcttca atggcatcga aggcacagag
    19261 acggggagag ggcgcgactc atcaaacgca acccagtcaa aacgctcgcg gttaacgata
    19321 tttttcagcc gcatccacag cagaggttgc gccagcagtg tacgcaaaga gatgatgaga
    19381 ataccgccat ttgcctgatg caccagacca ggctgcaggg taatgtcgcc attaaactgg
    19441 cgcaggcagc caaagagttg ctccgcttct acccagtcgg cagcgacaac ttgcgttaaa
    19501 gtcgcaaaat tatcatctgc actcactgcg ttacgtaagc ggatggtgtg gccggaaacc
    19561 tcatagtgac cgcccaccag ttggcctgca tcgctttgca gcgtacgcgc ggcatcggca
    19621 ataagattca gatactcaga ctcttccggg gccttcgcca gcataaaaga ggaggatgct
    19681 cgcgtatgca gcaactgctc cagcgcaaat tgcagtcgcg gttgagtatc actgaataaa
    19741 ggatcgtttt cgtcaatcaa atgtggctga gcaaatattt cctgatagct atcggtatca
    19801 ggaaccaggt cacgccatgc aagtttcgta atggtcaaag ttgatgtttt ttagtctgtt
    19861 gtcaaagccg cgattatacc gtaaccggca ctacagcaca cgtagaaagc accgacaata
    19921 ctcctggcat gggcgttaaa gctcacagga tggagattct tttcttcact ggcctaaaaa
    19981 gctgatattc tgtaaagagt tacacggtaa cattgagatc gctatgaaat atcaacaact
    20041 tgaaaatctt gaaagcggtt ggaaatggaa gtatctggtg aagaagcatc gcgaagggga
    20101 gttaatcacc cgttacatag aagccagtgc cgcccaggaa gccgtcgatg tgttgctctc
    20161 gctggaaaat gaacccgtgc tggtaaatgg ctggattgac aagcatatga atccggaact
    20221 ggtcaatcgc atgaagcaga ccattcgggc cagacggaag cgccatttta atgcggaaca
    20281 ccagcatacg cgcaaaaaat cgatcgatct ggaatttatt gtctggcaac gtctggctgg
    20341 tcttgcgcag cgtcgcggta agacgctttc tgaaacgatt gttcagctga ttgaagatgc
    20401 ggaaaacaaa gagaaatacg cgaataaaat gtcttctttg aagcatgatc tgcaggcatt
    20461 gctgggtaag gaataactga cgaagtcagt tcaatttact aaaggcgaaa aaaaaccccg
    20521 cagctgcggg gtttttctac cagacgagaa cttaagcctg cggctgagtt acaacgtctt
    20581 tgatgccttt aacttcgatc tctacgcgac gatccggagc caggcagtcg atcagtgcag
    20641 cacgctgttt cacgttgtca caggtgttgc cagtaacagg gttggattcg cccataccac
    20701 gtgcggagat cttgtctgcc gggatacctt tggagatcag gtaatcaaca acagactgag
    20761 cacggcgctc ggacagcccc tggttgtaag cgtcagaacc gatgcggtca gtgtaaccca
    20821 gaacaactac ggaaccgtct ttcggatcca ggttgctcag ctggctgtac agctgatcca
    20881 gagcagcctg accttccggt ttcagggttg ctttgttgaa gttgaacaga acgtcagact
    20941 tcagagtgaa gtgcttggtc tgtacttccg gtgccggagc tggagccgga gcaactaccg
    21001 gagctgcttc gccctgaccg aaacggtagg aaacacccag gctcagcaga ccgttgtccg
    21061 gacgagtacc aatggtgttt gcgtcaccga tgttgttggt ccactggtat tccagacggg
    21121 tagcgatttc aggagtgatc gcgtactcaa caccacctgc gaaaaccgga gaaacgccgg
    21181 tgtcgtggtc tttaaaggat gcgccaccag gtacgttagc cttggtgtct gcacgccata
    21241 ccataccacc cagacgagtg tagatgtcca gatcgtcagt gattgggtaa cccagtttag
    21301 cggtcagctg aacgccctga gctttgtatg cgccattgat gttgtcgcct ttgtacggca
    21361 tacgacctaa ccagtcgtaa cccatttcaa agccaacata cgggttaacc tggtaaccac
    21421 caaaagcacc agcgcccagt tggttttcat gggtcgggcc attgttgtcg atgaaaccag
    21481 tgtcatggta ctgggaccag cccagtttag caccagtgta ccaggtgtta tctttcggag
    21541 cggcctgcgc tacggtagcg aaaccagcca gtgccactgc aatcgcgata gctgtctttt
    21601 tcattttttg cgcctcgtta tcatccaaaa tacgccatga atatctccaa cgagataaca
    21661 cggttaaatc cttcaccggg ggatctgctc aatattaact ctaccgatat cttcggctta
    21721 tgccgagcac ccctggcgat gtaaagtcta caacgtagtt gaaaacttac aagtgtgaac
    21781 tccgtcaggc atatgaaaaa aaagtcttgt ataaggtacg tttaatcttt tttgtcagcg
    21841 acaatttaca gaagaaaatc gcggaaaccg cttcagacaa gcctccgcaa ggaaaattag
    21901 tcacgactga aagcattggc tgggcgacaa aaaaagttcc aggattaatc ctaaatttac
    21961 ttaatgatac aaattagagt gaatttttag cccggaaagt tgtctcgttg cgtgagagga
    22021 tgcgcttacc ggacgcataa taaaccccat agcgttacct tcatttgccg catctacaag
    22081 ttcagcatgc tcttcttcag tcaaatcatc tgccaaccaa ccgatcacca cactgtaatt
    22141 gcccgtgcgt aaagcgcgaa ccattgactc cacagtgtgg caagggggga gctggctaat
    22201 ctgcattact ttcgttaagg gtagcccaga tgcctgaacc cattcccgac tcagtttttg
    22261 ttgcggtgtt aaccagagtt gccagcgcga ttgctgaccg agttgctgta acaatggcaa
    22321 cagtagaagt tgcgtcatca tgggctgatc ttcgcgatag acaacttcac tgataagccc
    22381 ggctgtagtg ttttccgtag agacacgcgc aattttactt gctgcggatg agaacgacga
    22441 agaacgatgt gcatagcctg aagtgtacat aatcaatcca gcccctgtga gttactgtat
    22501 ggatgtacag tacatccagt gacgacaaag atcaacccta ttttcggaaa gagcctcgca
    22561 aattttgtcg ttggtgacgg gaaaacataa attaatcttg cccctaaaga ataagttgcc
    22621 tattttcgta tttaacggat ccgttaatgt gaatcattct tttatgttat gattttaaaa
    22681 ggaattttat gaaaagcctc tcctataagc ggatctataa atcacaagaa tacctggcaa
    22741 cgttgggcac aattgaatac cgatcattgt ttggtagtta cagcctgacc gttgacgaca
    22801 cggtgtttgc gatggtttct gatggtgagt tatatcttcg ggcttgtgag caaagtgcac
    22861 agtactgtgt aaaacatccg cctgtctggc tgacatataa aaagtgtggc cgatccgtta
    22921 ccctcaatta ctatcgggtt gatgaaagtc tatggcgaaa tcaactgaag ctggtgcgtc
    22981 tgtcgaaata ttctctcgat gcagcgctga aagagaaaag cacgcgcaat acccgggaaa
    23041 gactgaaaga tttgcccaat atgtcttttc atctggaagc gattctcggg gaggtgggga
    23101 ttaaggatgt acgggcgtta cgtatacttg gggcaaaaat gtgttggttg cgactgcggc
    23161 agcaaaacag tctggtgaca gaaaagattc tgtttatgct tgaaggtgcc attatcggca
    23221 ttcatgaagc tgcgctcccg gtggcacgcc gccaggagct tgcagaatgg gctgactctc
    23281 ttacgccgaa acaggagttt cctgcggaac ttgagtaatc tggcgttgca gacgaccaat
    23341 ctcaggcagt aatgcaatca ataacccaac ttgttgtacg accagaggtt ctttgctgtc
    23401 tgcccgtggt tcaagttgct gcatccgctg tttcaggcca gctaatgcct gattgacgcg
    23461 ttcttcatca gcaggttgat gatgtaacgc gtcatcaaca tagcaaactg cgtcatcaag
    23521 aaacgccaga atttcaggat tagttaactg ctcccggtga gcaccgaggg ctgagatata
    23581 gctggtaaac gtatggttaa ggcacagcaa ccgaaacgcg gcttcgcgaa tttgcggggt
    23641 aacgttcggt tcgctggaca tatttgatac caccgacgcc agttcagcat cacggttgtg
    23701 tgcatcgcgg cgggcaatac gatacgccag acggttatca cgcccctgat ggtattgctc
    23761 cagtatggca tcgagatagc gacagttggc ctctgtggcg cgttcgagca tgcgcggcag
    23821 attgcgaaac tgccagtcag gccagatgta gctcactgcc gcccaggcaa tggcacaacc
    23881 aatcagcgta tcgataacgc gaggtaacgc tacttcaaaa ccttcaccca gtaagttaaa
    23941 acacagtagc accaaaagtg tgatgaacat cgttgcatga gcgtattgca cgttacggaa
    24001 ggcaaaaaag agcacgccgg taataaccag cagcaccagc tgcccttcca gtgatggcac
    24061 aaaccacagc acaggaatgc caatggcgat acctaccagc gtaccaataa tccttaactt
    24121 caggcggtgg cgcgtggcgt tatagtttgg ctggcagaca aacaaacttg tcagcaagat
    24181 ccaatacccg tgatgcattc cggttatttg aatgatggcg tagccgaagc acaacaccag
    24241 cgacattctt accgcatgac ggaagagggc ggattccggc gtgaagtgac ggctaagacg
    24301 cagccagata tcactcaatc cgtgcgggct gtcatcagcg agctcatttt cgtcattatt
    24361 atggggcagt gcctgggcct gttctgattc aattgttgcc agttgggcat caatggcgcg
    24421 taaattgttc agcaaaaatc ccagtgtttt gagtaaatcg gcaggcgcgc cgttatcgcg
    24481 catccgctcc agcgcagcat caatatgcgt aaaagcgcgt tcaaaatgcg gatcatgttg
    24541 ataaggctga cgcaacaaaa tacagcgtga cagttgctgg cacgcctggc cctgcatcga
    24601 catcagccgc tgaaaacgga acagcacgtc gctgtggcga aaatgttcac gcaatgtttg
    24661 atactgaata tgagaagagc tggcacgctc gtgaatatcc tgtgcgacaa agtaataatg
    24721 cagcgtgcga cgcgttcccc gttgaccacg atcgccacgt aagcgggtca gcagcgagag
    24781 tttcgtctga ttcaatgtcg ccatcagctg accgttggcg agagccaaat cgtacagcgg
    24841 tgcctggctt tcatcttcaa tatcaggatc aaacatgcgc gacttgagct caagataacg
    24901 cgccagttgt tcatagcaac gcgccaggtt gtcctgcagc gggcggaccg ggaacagcag
    24961 atgaccaata agtgtcagga cgttgtacca gacggcaccg gccagcagat acatcggctg
    25021 ctgataccag tgctcataca gtgatgttcc caacattgtg taaatggcga tcagcaatgc
    25081 accgaaggca attgttgcat agcgttgacc cagaccgccg agcaaaatga agccgctggt
    25141 agagagcgtt aagccaatcg caaatagcca gggccaggga aacagcaatt ctactgaggc
    25201 cgaggcgata aaaaagcaga acagcgtaat gatgaggtta cgcaaacgtc ccgccagtcg
    25261 gtcatcgaga tcggtcagcg ccgctgccac catccccagc gtcagcggaa tcgtcagttt
    25321 tacatcaccc agccaccacg gaaacgctgt ggttccacaa agcgcaataa aaatacgcgc
    25381 gtaatacagc caggcgctgt tccaggtata gcgtttgagc aaaggactta gcatataggc
    25441 catattagtg gattattcaa aacgacgacg tgcattggct tcacgcgcag cttgcgctgt
    25501 ttctaccgat accacgcgac gaccaaccgg ccatagtgca atagcggcaa ttttaaagtt
    25561 cgcaataccg acaggaatgc caatgatgga aatacattgt gcgatgcccg ttgcaatgtg
    25621 catcaggcat aaccaccagc caaagaaaat cagccagaaa atattcaata ccgtaccgcc
    25681 agtattcagc agcacatttt tgccagccgg gttcagttca tcgacatgaa tagcttcatt
    25741 gccataaggc accagagaca gtttagtgat ctcccagcag gatcgtgtca gcggtaaggt
    25801 aaaaatcagc acaatactga ccagagtcgc caacagccag cccagagtgg tggcaaatcc
    25861 gccaagcaca aagttcagaa tgttcaaaac ggtacgcata aaacctcgct ttactgtggt
    25921 tttcagtaat gggcggcaat tgtaacgttt ttttgggctg gagcacgttt tctctgacgg
    25981 ttacactgat aagaaataat ttcgtgtgga tctacagagt catggaactg aaagcgacaa
    26041 cgcttggaaa acgtctggca cagcaccctt acgatcgggc ggtgatcctt aatgccggga
    26101 ttaaagtctc cggcgatcgc cacgaatacc ttattccttt caatcaatta ctggcgattc
    26161 actgtaagcg cggtctggta tggggcgagc tggaatttgt actgccggac gaaaaagtgg
    26221 tgcgtctgca cggtaccgaa tggggcgaga cgcagcgttt ttaccatcat cttgatgctc
    26281 actggcggcg gtggagtggc gagatgagcg aaattgcgtc tggtgtttta cgccagcaac
    26341 tggatttgat tgccacgcgc actggagaaa ataaatggct gacgcgtgag caaacctctg
    26401 gcgttcagca acaaatccgc caggctttgt cggcgttgcc gttgccggtt aaccgactgg
    26461 aagaattcga taactgccgt gaggcgtggc gtaaatgtca ggcctggttg aaagatattg
    26521 aaagcgctcg gttgcagcat aaccaggcgt ataccgaagc catgcttacc gagtatgcgg
    26581 attttttccg ccaggtcgag tcttcaccgc tgaatccggc gcaggcccgg gcagtcgtta
    26641 atggcgagca ttctctgtta gtgctggcag gtgcaggaag cggaaaaacg tcggtgctgg
    26701 tggcccgtgc tggctggttg ctggcgcgtg gtgaagcgtc ccctgagcaa attttattgc
    26761 tggcgtttgg tcgcaaagcc gctgaagaga tggacgagcg tattcgcgaa cggctacata
    26821 ccgaagacat taccgcacgc acgtttcatg cgctggcgct gcatattatt cagcagggca
    26881 gcaaaaaagt tccgatagtc agcaaactgg aaaatgatac cgctgcccgt catgaactct
    26941 ttattgctga gtggggcaag caatgcagcg aaaagaaagc gcaggcgaag ggctggcggc
    27001 aatggctgac ggaagaaatg cagtggtcag tgccagaagg taacttctgg gatgatgaaa
    27061 aattacagcg tcgtcttgcc tcacgcctcg atcgttgggt aagtctgatg cggatgcacg
    27121 gtggtgcaca ggcagaaatg attgccagtg cacccgaaga gattcgcgat ctgttcagta
    27181 aacgtatcaa gttgatggcc ccgttattaa aagcctggaa aggtgcgctg aaggcagaaa
    27241 acgctgtcga tttttcgggc cttattcatc aggcgattgt gattctggag aaaggtcgct
    27301 ttatcagccc gtggaagcat attctggttg atgaatttca ggatatctcg ccgcagcggg
    27361 cagcgttgtt agcggcatta cgcaagcaaa acagtcagac gacgttgttc gccgttggtg
    27421 atgactggca ggcgatttac cgattcagcg gtgcgcaaat gtcgctcacc accgctttcc
    27481 atgaaaactt tggtgaaggc gatcgctgtg atttagacac gacttaccgt tttaacagtc
    27541 gtatcggtga ggtggcaaac cggtttattc agcagaaccc aggccagctg aaaaagccgc
    27601 taaacagctt aaccaatgga gacaaaaaag ccgtcacgtt attggatgag agtcaacttg
    27661 acgctttgct ggataagctc tctggttatg ccaaaccgga agagcgcatt ctgatcctgg
    27721 cgcgttacca tcacatgagg cctgccagcc tggaaaaagc ggcaacacgc tggccgaagt
    27781 tgcaaatcga ctttatgacc attcatgcca gcaaagggca acaggcggat tacgtcatca
    27841 tcgttggctt gcaggaggga agtgatggtt ttccggctgc ggcgcgggag tcgattatgg
    27901 aagaggcgct actgccaccg gttgaggatt tcccggacgc tgaagaacgg cggttaatgt
    27961 acgtggcgct gacccgggca cgccatcggg tatgggcact gtttaacaaa gagaatccct
    28021 ctccctttgt ggaaatactg aaaaatctgg atgtgccggt ggcgagaaaa ccgtaagaaa
    28081 caggtggcgt ttgccacctg tgcaatatta cttcagacgg tccgcgagat aacgctgata
    28141 atcggggatc agaatatcga ccgcgtcgtt gaaatgcggc gactggataa tgaagtctgc
    28201 cgttgccacg ttggtggcga ccggaatgtt ccataccgtc gccagacgca gcaaggcttt
    28261 cacgtcagga tcgtgcggta cggcatttag tggatcccag aagaaaatca atacatcaat
    28321 tttcccttct gagatcaatg cgccaacctg ctggtcaccc cccattgggc cactcaacat
    28381 cgcgttgacg ttcatgccgg tcgcgcggga aattaagtta ccggtagtgc ctgttgcata
    28441 cagtacgtgt tgttccagta acggttgatg tcgttccacc cagctcatca gcatttgttt
    28501 gcagtgatcg tgtgccacca gcgcaatatg tttccgcgca ggtaaagtgc gagtcgtcag
    28561 ttccataatg tacatccgta gttaactttc ctacagatta ctgtaagcac ttatcgctgc
    28621 aagttaaaga ccgaaaaagc ctgcgcacag gcacaaaaat ctcaggaaga tggttgtttt
    28681 tccgcccatt gcaggaaagt atttcgcgtt tgtgggtcag ccagtttaaa ccaatacttc
    28741 agccgttgtt ctgtgagcac ctgagactgc ggtggaatag catccagctc ggaaacaccc
    28801 gaaagcagcg tactgtcatc tgccatcatc gtggcaaatt gcggtagtga agcgcgtttc
    28861 gcggatttgt tatagcgttc aacctcgacc tcataatcaa tcgtttttgc cgttgaggta
    28921 atggcgagaa tatccagctt taccggaatc ggcgtcgcat cgccatccaa caattcaagg
    28981 cgcggcgcgg catcaaaatg gttagcttcc cgctcattct ccaggcgagg caggcgaaaa
    29041 ttgacctggt tgatgagctg ggtattaaaa ctgaccacca gcggtgggga gatatacagc
    29101 cgttcttcac tattggaaag atgaatcgtc ttctcaacgc gaaacactaa ctgatgcggt
    29161 ccattatcca gttcaatgct gtccgcaccg cgcagcagag agctggaaac cttcttgccg
    29221 tcgagtacca gaagatcgac atcggttgaa agccgcagcg tggtggcaaa aacggatacc
    29281 ggcaaacata atgcaatcaa ggtggtcacg atgccggttt tcataggtag ctcctgtcaa
    29341 aagaccgccc gaataataat gtgtcaaaat ttttctttaa tcatacttac taacatatag
    29401 acatatttcc cgcttttgct ctcattcatt cgtattagct gcatggttgg catgtcggct
    29461 ttgtcgtaca ctttgttaaa cagtctggag gaaataatga aagaaaccga tattgcaggc
    29521 attttaacgt ccactcacac cattgcgctg gtgggggcaa gcgacaaacc cgatcgtccc
    29581 agctatcgcg tgatgaaata tctactcgac caggggtatc acgtcattcc ggtttcgcca
    29641 aaagttgccg gcaaaacgct gctgggccag caggggtatg gcacgctggc agacgttccg
    29701 gaaaaggtcg atatggtcga tgttttccgt aattcagaag cggcgtgggg cgtggcacag
    29761 gaagctattg ccattggcgc gaaaaccctc tggatgcaac tgggggtgat taatgagcaa
    29821 gcggcagtac tggcacggga tgccgggttg aatgtggtga tggatcgctg cccggctatt
    29881 gagatccctc gcctgggcct ggccaaataa aaaatccccg gaaggcaaaa accttccggg
    29941 gatttgttca gggattagtt acgcagacgc ggggcctgga gttgtttgcg gatggtctgc
    30001 gccagctcat ccatggatgg ctgttcagga tgttcatcct gcaattcgct acttagctgg
    30061 gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt cctccatcac cacgtgatac
    30121 cacggagcgg cgcgaagctc gtcattcacc gccagctcat caggcgacgg ttcagagagc
    30181 gaataaaccg ggtcgatatc cacgaccact ccgagataac ctaacaggga atggcggacc
    30241 tgctggccga taccgaattt gctggcaatc atagtcacct cccgggaaat ctttaccttt
    30301 gatataaggg taaaattcca catttcaagt tacatgacgc gacaggcaaa ccctttcaga
    30361 tatagccctt ccggataggt agcgatcacc ggatgatcgg ccgcctgacg gaactgctct
    30421 ataaattgta catcacgacc ggcatcaatt gcggcatccg cgatgatttt ctgaaataaa
    30481 tcgctggtca tcagaccgga acaggagaaa gtcaggagaa taccgccttc attcagcagc
    30541 tgaatcgcca gcatgttgat gtctttataa ccacgacacg cgcccatcaa ctggctttta
    30601 ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa atttttcacc gcgatcgcga
    30661 taagtacgca gcaatttaaa gacatcatca cggacaaact ccgccttgct cagatccagt
    30721 ttgttcagct caacgttctg ccgtgcaata tccagcgctt cctgggaggt atcaacgctg
    30781 acaacctggc tgcaaccgcc catcagtgcc gatacggcga aaccaccggt ataggagaaa
    30841 cagttcagta cgcgtttatt ttcaacgtag cggcgggtag ccaggcggct gtctcgctgg
    30901 tccaggtagt agcccgtttt gtgtccgtgc tgtatgtcca ccagcagctt catgccatgt
    30961 tcttcaatcg gcagcagggc aggcggcaac tcgccggtga cggggccctg ggtcagctcc
    31021 atcccttctt ttttacgtac cgcaacatcg ctgcgatcgt aaatcgcaca ttccgggtac
    31081 agcgtttgca gggcactaat taatgccgcg cgctgatatt ctgcgccagc actcagcagt
    31141 tgcagcacca gaaaattacc gaaacgatcg atagtaatac ccggcaggcc atcagattca
    31201 ccggcgatta aacgatagct gtcgaggcca tctttttgcg ccagccagtc acgccatttt
    31261 tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga tagactcaga cgggtcaaac
    31321 gtccagacgc gcgcccggat ttgcgaagct ggcgaataag cgccgcgtgc taaccatttt
    31381 ccctgatgat caacaatatc gatggtttca ccgaggctgg ctttaccttc catgcgggca
    31441 acggccccgg aaaagaccca cggatggcga cgaagtaatg atttttcgcg ccctttggct
    31501 aacactaaac gtacactcat aatttacttt tctgtcgatg caaagaaatg ggcgtcattg
    31561 tccggagttt tccatggatt tgcaacgcgt ccattaagga taaggagagc agcaatgtcg
    31621 aaagtctgca taattgcctg ggtttacggg cgagttcagg gcgtaggatt tcgctacacc
    31681 acacagtacg aagcgaaaag actgggttta accgggtacg ccaaaaatct tgacgatggc
    31741 agcgtggaag tggttgcttg cggtgaagaa gggcaggtag aaaaattaat gcagtggtta
    31801 aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc tcagcgagcc gcatcatccc
    31861 tccggtgaat taaccgattt tcgtatccgt taaatacact ttaccggttt tggcaggcca
    31921 gcaattttgg tggcttgctt agccggacct ttcggaaaca gtcggtataa atagcggctg
    31981 ttaccttttt cttcgccaaa tttattcgcc atcgctttta ccagcatacg aatcgccgga
    32041 gacgtattaa attccagata gaaatcacgc acaaaacgca ccacttccca gtgttctggc
    32101 gacagcgaaa tcccttcgtt ctctgcaatc accactgcca gcggctcact ccactggctg
    32161 ctttctttga gatagccttc ggtatccgtt tctatctctt taccttcgaa gatcagcata
    32221 attattacta ccttaatcag actgccggca gtgtaacaaa aaacaaagcc ccgcataaag
    32281 cgaggctatg acagtgttag cgggtgagat taatcgcggc tggcgaagcc cagaatgctc
    32341 agcaggctga cgaagatgtt gtacagcgaa acatacaggc taaccgtggc acgaatatag
    32401 ttcgtttcac cgccatgaat gatgttgctg gtttcaaaca aaatagcgcc agaggagatc
    32461 aaaatgaaga ccgcgctgat cgccagatgc agagcaggca gctgcaggaa gatattagca
    32521 accataccaa tcagcaccac cacaataccc gccatcagca taccgccgag gaacgacata
    32581 tctttgcggg tggtcagcac atatgcagag cagcagaaga acactaacgc cgttccgccc
    32641 agtgccatag cgattacgtc acccattccg gcagacagat aggtgttcag aattggtccg
    32701 aggatataac ccagaaaacc ggtaaaggcg aatgcggaga taatcccggt cggcttattc
    32761 gccgttttat aggtcaggaa catcaaacca tacataccca ccagcgtcag aatcagaccc
    32821 ggagatggca gcatcagcac cgtactggca gttgcggtaa tcgccgaaaa ggccagcgtc
    32881 aggctcagca gaaaataggt attacgcagc accttatggg tgctaagcag tgatgtacgg
    32941 tcatgtgaag aactaacaat acgatccatt gagtcactct ctatgacaga tgtaattaat
    33001 taagcagcat aatgataatg cgtaagggca cccagaagtt ttacccatct ttacgcattt
    33061 gatctggaac aggtttaaca gcggattatc aggtcattaa gcaaatataa cgccctgaga
    33121 atttcgacag gcaaaagaaa aaggggttag catttagcta accccttatc ttatctggcg
    33181 gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc cggttttcaa gaccggtgcc
    33241 ttcaaccgct cggccacact tccggaatga cgcgcactat aaacatcccg atgcggcgtg
    33301 taaaccccta atttgtttgt ttgcctgaaa aacagccaaa agtgcattga tagcgtgaaa
    33361 taacagcaga ttgatcattt catcaccatg aattccttct cttttactcg tttagcaact
    33421 ggctaaacat cccaccgccc ggcaaaaaga aaaataggtc catttttatc gctaaaagat
    33481 aaatccacac agtttgtatt gttttgtgca aaagtttcac tacgctttat taacaatact
    33541 ttctggcgac gtgcgccagt gcagaaggat gagctttcgt tttcagcatc tcacgtgaag
    33601 cgatggtttg ccttgctaca gggacgtcgc ttgccgacca taagcgcccg gtgtcctgcc
    33661 ggtgtcgcaa ggagaagaga cgtgcgatat gaataacgag gaaacatttt accaggccat
    33721 gcggcgtcag ggcgttaccc ggcgcagctt tctcaaatat tgtagtctgg ctgccacgtc
    33781 gctgggatta ggcgcgggaa tggcaccaaa gattgcctgg gcgctggaga acaaaccgcg
    33841 cattccggtg gtatggatcc acggtctgga atgcacctgc tgtaccgaat cttttatccg
    33901 ctccgctcac ccgctggcaa aggacgtcat cctttccctg atttccctcg attatgacga
    33961 tactttgatg gctgccgccg gaacccaggc ggaagaagtc tttgaagaca tcatcacgca
    34021 atacaatggc aaatatatcc tcgcagtaga aggtaatccg ccgctgggcg agcaggggat
    34081 gttctgtatc agcagcggtc gaccgtttat tgagaaactc aaacgtgccg ctgccggagc
    34141 cagcgcgatt atcgcctggg gaacctgcgc gtcctggggc tgcgtgcagg ccgcgcgacc
    34201 taatccgaca caggcaacgc ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt
    34261 acctggctgc ccgccgatcc cggatgtgat gagcgccatc attacttaca tggtgacctt
    34321 tgatcgcttg ccagatgtcg acagaatggg tcgtccactg atgttttatg gtcagcgtat
    34381 ccacgataaa tgctatcgcc gcgctcactt cgacgccgga gagttcgtcc agagttggga
    34441 tgatgacgct gcccgcaaag gttactgcct gtacaaaatg ggctgcaaag ggcctaccac
    34501 ctataacgcc tgttcctcca cacgctggaa tgatggcgtt tctttcccaa tccagtctgg
    34561 tcacggctgc ctgggctgtg cggaaaatgg tttctgggat cgcggttcgt tctacagccg
    34621 cgtggtcgat attccacaaa tgggtactca ttccaccgcc gataccgtcg gtttaaccgc
    34681 gcttggtgtg gtggcagcgg ctgttggtgt gcacgcagtc gccagcgccg ttgaccagcg
    34741 cagacgtcat aatcagcaac ctacagaaac cgaacatcag ccaggcaatg aggataaaca
    34801 ggcatgagca ctcagtacga aactcaggga tacaccatca ataatgccgg acgccgcctg
    34861 gtggtcgacc cgattacgcg cattgaaggc cacatgcgct gcgaagtgaa tattaacgat
    34921 cagaatgtga tcaccaatgc cgtctcctgc ggcaccatgt ttcgcgggct ggagatcatc
    34981 ctgcaagggc gcgacccgcg cgatgcgtgg gcgttcgttg aacgtatctg cggcgtctgt
    35041 actggcgtac acgccctggc ttcggtttac gccatcgaag atgccatcgg tattaaagtg
    35101 ccggacaacg ccaatatcat ccgcaacatt atgctggcaa cgctctggtg ccacgatcat
    35161 ctggtgcact tctatcagct tgccgggatg gactggatcg atgtgttaga tgcgctgaaa
    35221 gccgacccgc ggaaaacctc cgaactggcg caaagtctct cctcttggcc gaaatcatcc
    35281 cctggttatt tcttcgacgt acaaaatcgc ctgaagaaat tcgttgaagg cggccagttg
    35341 gggattttcc gcaatggcta ctggggtcac ccgcagtaca aactgccgcc agaagccaac
    35401 ctgatgggct ttgcccacta tctcgaagct ctcgatttcc agcgtgaaat tgtcaaaatc
    35461 cacgcggtct ttggcggtaa aaacccgcat ccaaactgga ttgtcggcgg gatgccttgc
    35521 gccattaaca ttgacgaaag cggcgcggtc ggggcggtca atatggaacg cctgaacctg
    35581 gtgcaatcga ttatcacccg cacggcagac ttcattaaca acgtgatgat ccccgacgcc
    35641 ttagccattg gtcagttcaa caaaccgtgg agcgaaatcg gcacgggtct ttctgataaa
    35701 tgtgtcctca gctacggcgc attcccggat attgccaacg actttggcga gaaaagtctg
    35761 ctgatgcctg gcggcgcggt gattaacggc gacttcaaca atgtgctgcc agtggatttg
    35821 gttgatccgc agcaggtgca ggagtttgtc gatcacgcct ggtatcgtta tcccaacgat
    35881 caggtcgggc gtcatccatt cgatggcatt accgacccgt ggtacaaccc cggcgatgtc
    35941 aaaggcagcg ataccaacat tcagcagctg aatgaacagg aacgctactc gtggatcaaa
    36001 gcgccgcgct ggcgcggtaa cgcgatggaa gtggggccgc tggcacgcac gttaatcgct
    36061 tatcacaaag gcgatgctac gaccgttgag tcggtcgatc gcatgatgtc ggcgctgaac
    36121 ctgccgcttt ccggtatcca gtcaacgttg ggccgcattt tgtgccgcgc gcacgaagca
    36181 cagtgggccg caggtaagtt gcagtatttc ttcaacaagc tgatgaccaa tctcaaaaac
    36241 ggcaatcttg ccaccgcctc cacggaaaaa tgggaaccca caacctggcc gacagagtgc
    36301 cgtggtgtcg gctttaccga agcgccgcgc ggggcgttag gccactgggc cgccattcgc
    36361 gatggcaaga ttgatctcta ccagtgcgtg gtgccgacca cctggaacgc cagcccgcgc
    36421 gatcccaaag ggcagattgg cgcttatgaa gcggcgctga tgaacaccaa aatggcgatc
    36481 cccgagcaac cgctggagat cctgcgtact ctgcacagct ttgatccgtg cctcgcctgt
    36541 tcaacacacg tgctgggcga cgacggtagc gagctgatct ccgtgcaggt gcgttaacag
    36601 cgaaggagaa tcatcatgca acagaaaagc gacaacgttg tcagccacta tgtctttgaa
    36661 gcgccagtgc gtatctggca ctggctgacg gtgttatgca tggcggtgtt gatggtcacc
    36721 ggttacttta tcggtaaacc gctaccttcc gtcagcggcg aggcgacgta tctgttctat
    36781 atgggctaca tcaggttaat tcacttcagc gccgggatgg tttttaccgt ggtcttgctg
    36841 atgcggatct actgggcttt tgttggcaat cgatactccc gcgagctgtt tatcgtgcca
    36901 gtatggcgta aaagctggtg gcagggcgtg tggtatgaaa tccgctggta tctgtttctg
    36961 gcaaaacgtc cgagtgccga tataggccat aaccccatcg cccaggcggc gatgtttggc
    37021 tatttcctga tgtcggtctt tatgatcatc accggctttg cgctgtacag cgaacacagc
    37081 cagtacgcta tttttgcgcc gttccgttat gtggtggaat ttttctactg gacgggaggc
    37141 aactcaatgg acattcacag ctggcatcgg ctggggatgt ggctgattgg cgcgtttgtg
    37201 atcggtcatg tctatatggc gctgcgtgaa gacatcatgt ccgacgacac ggtgatctcc
    37261 accatggtca acggctaccg tagccacaaa tttggcaaaa taagtaacaa ggagcgttca
    37321 tgagcgagca acgcgtggtg gtcatggggc tgggcaacct gctgtgggcc gatgaaggtt
    37381 tcggcgtgcg ggtggcggaa cggttgtatg cccattacca ctggcccgag tatgtggaga
    37441 ttgtcgatgg cggtactcag ggactgaact tgctggggta tgtcgaaagc gccagccatc
    37501 tgttgattct cgatgccatt gactacgggc tggaacctgg aacgctgcga acctatgccg
    37561 gagaacgtat tccggcttat ctcagcgcga agaaaatgag cctgcaccag aacagtttct
    37621 ccgaagtgct ggcgctggca gatatccgcg gacatctgcc agcacatatt gccctcgtcg
    37681 gtctgcaacc cgcaatgctc gacgactacg gcggcagcct gagcgaactg gcacgggagc
    37741 aactgcccgc agcggaacag gcggcgctgg cgcagctggc tgcatgggga attgtgccgc
    37801 aaccggctaa tgaatcgcgc tgtctcaatt atgactgtct gtcgatggaa aattacgaag
    37861 gcgttcgctt gcgccagtac aggatgacac tggaggagca gggatgagca acgacacgcc
    37921 atttgatgcg ttgtggcaac gaatgctggc gcgcggctgg acgccggtta gtgaattccg
    37981 tcttgacgac tggcttacgc aagcgccaga cggcgtggtg ttattaagca gtgacccgaa
    38041 acgcacgcca gaggtcagcg ataatccggt aatgattggc gaattactgc gcgagtttcc
    38101 cgactacaca tggcaggtgg cgattgctga ccttgaacag agcgaagcca tcggagatcg
    38161 ctttggcgtc tttcgctttc ccgccacatt agtgtttacc ggcggaaact atcgcggcgt
    38221 gctgaatggt attcacccgt gggcggaact gataaacctg atgcgtgggc ttgtcgaacc
    38281 acaacaggag cgtgcctcat gagcgaaact tttttccatc tgctggggcc aggaacgcaa
    38341 ccgaacgatg acagtttcag catgaatcca ctgccgatca cctgtcaggt gaatgatgaa
    38401 ccgagtatgg cggccctgga acagtgtgct cacagcccgc aggtgattgc gctgttaaac
    38461 gagttacaac atcaactaag tcaacgccag ccgccgttgg gcgaggtgct ggcagtcgat
    38521 ctgttaaatc tcaacgccga cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa
    38581 gtgtcagtgc gcattcagca ggctgacgac agcgaaagtg aaatacagga ggcgatcttc
    38641 tgcggattat ggcgggtgcg cagacgtcgc ggcgaaaagt tgctggagga caaactggag
    38701 gccggctgcg cgccgctggc gttgtggcag gcggccacgc aaaatctctt gccgacagat
    38761 tcgctgttac cgccgcccat tgatggcctg atgaatggcc taccgttggc gcatgagtta
    38821 ctggcgcatg tacgtaaccc cgacgcgcag ccgcacagca ttaatctgac gcaattaccc
    38881 atcagcgagg ctgatcggct ttttctctca cgtctctgtg ggccgggaaa tattcagatt
    38941 cgtaccattg gctatggcga gagctatatc aacgccacgg ggttacgcca tgtctggcat
    39001 ttacgctgta cggacacctt aaaaggcccg ttactggaaa gttatgaaat ctgcccaata
    39061 ccggaagtgg tgctggcagc gccagaagat ttggtcgact ctgcgcagcg gcttagcgag
    39121 gtatgtcagt ggctggcgga agctgcaccg acgtaaaaag acggtaagta tcgctttcag
    39181 tcttatgaat atcgcaatcg gcgaatacct ctggtcgtag agtttcagga taaagaggga
    39241 gatctaccat tatcgggtta tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt
    39301 cattgattta tcgcgctggc agtttgctct gaccgcgctg tatcactttt tatttgtacc
    39361 ccttaccctg gggctgattt ttttgctggc tatcatggaa accatttacg tggtcaccgg
    39421 caaaacaatc taccgcgata tgacgcgctt ctggggtaag ctcttcggta tcaactttgc
    39481 tcttggcgtg gctaccggcc tgaccatgga gtttcagttt ggtactaact ggtcattcta
    39541 ttccaactat gtgggcgata tttttggcgc accgctggcg atggaagcat taatggcctt
    39601 cttcctcgaa tccacctttg tcgggctttt cttcttcggc tggcagcggc tgaataaata
    39661 ccagcacctg ctggtgacgt ggctggtggc gttcggttca aatctctctg cattgtggat
    39721 attgaatgcc aacggttgga tgcaataccc gaccggtgcg cattttgata tcgacaccct
    39781 gcgtatggag atgaccagct tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt
    39841 tgtgcacacc gtaatggcgg gctacgtgac cggggccatg tttattatgg cgatcagcgc
    39901 ctggtattta ctgcgcggac gggagcgcga tgtcgcatta cgctcgtttg ccatcggttc
    39961 cgtcttcggt actctggcga ttattggcac gctgcaactc ggagacagtt ctgcgtatga
    40021 agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa ggggagtggc aaacggaacc
    40081 tgcacctgca ccgttccatg tggttgcatg gccggaacag gatcaagagc gtaacgcctt
    40141 tgccatcaaa attcccgcgc tgctagggat cctcgccact cactcattag ataaacccgt
    40201 gccgggtctg aagaatttga tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc
    40261 ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag ccgcatgtgt tgcagtcatt
    40321 ccgggagctg gaaggcgacc tgggctacgg catgttgctc tcccgctatg cgccggatat
    40381 gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc
    40441 gccggtattc tggagtttcc gcatcatggt gggctgtggt tccctgctgc tactggtgat
    40501 gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa
    40561 aatggcgctc tggagtttgc cgttgccgtg gattgcgatt gaagccgggt ggtttatgac
    40621 cgagtttggt cgtcagccgt gggcgataca ggacatctta ccgacatact ccgcgcactc
    40681 cgctttaacc acaggacaac tggctttctc actgatcatg atcgtagggc tttacaccct
    40741 gttcttaatc gccgaagtct acctgatgca gaaatatgcc cgtctggggc cgagcgcgat
    40801 gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa
    40861 acattgcgct tcatctggtg gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc
    40921 gacggatttg acatggggat tggctgtttg ctgccgctgg tggcgcgtga tgatgatgaa
    40981 cgccggatag tgattaatag cgttggtgcg cactgggaag gcaatcaggt ctggttgatc
    41041 ctcgcaggtg gggcattatt tgccgcctgg cccagagtgt atgcagcggc gttttccggc
    41101 ttttatgtgg cgatgatcct ggtgctgtgc tcgctgttct tccgcccgct ggcctttgat
    41161 tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt gggatgccgg tctggtcatc
    41221 ggcagtctgg tgccgccggt agtcttcggt atcgccttcg gcaacttgtt gctcggcgtg
    41281 ccgtttgcct tcacaccgca attacgcgtg gagtatctcg gcaacttctg gcaactgctg
    41341 acgccattcc ctttattgtg cggattgctc agccttggga tggtgatttt gcaaggtggc
    41401 gtctggttac aactgaaaac tgttggtgtg attcatctgc gttcacagct ggcgaccaaa
    41461 cgcgccgcac tactggtgat gctgtgcttt ttgctggcgg gttactggct gtgggtcggt
    41521 attgatggct ttgtactgct cgcccaggat gctaacggtc cttccaatcc gttaatgaaa
    41581 ctggtggcag tgctacctgg tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg
    41641 atcttcccgc tgctggggtt cttctgccca ttgctgacgg tgatggcgat ttatcgtggt
    41701 cgcccgggtt ggggattttt gatggcgtca ttgatgcaat ttggcgtgat tttcacggca
    41761 ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga gtccaatctc cagcctgacg
    41821 ttgtgggaca gcacttccag tcagctgacg ctgagtatta tgttggtaat cgtgctgata
    41881 tttttgccca ttgtgttgct ctacactctc tggagctact acaaaatgtg ggggcgcatg
    41941 acgacagaaa ctctccgccg taacgaaaac gagctgtact aaggagcagg aacaatgtgg
    42001 tatttacttt ggttcgtcgg cattttgttg atgtgttcgc tctccaccct tgtgttggta
    42061 tggctggacc cgcgattgaa aagttaacga acgtaagtct gatgcggcgc attagcgtcg
    42121 tatcaggcaa tcaataatgt cggatatgaa aagcggaaac atatcgatga aagcgatctt
    42181 gatcccattt ttatctcttc tgattccgtt aaccccgcaa tctgcattcg ctcagagtga
    42241 gccagagccg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg gtgtgcgtgc
    42301 cccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc caacctggcc
    42361 ggtaaaactg ggttggctaa caccgcgcgg tggtgagcta atcgcttatc tcggacatta
    42421 ccaacgccag cgtctggtag ccgacggatt gctgacgaaa aagggctgcc cgcagcctag
    42481 tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg aagccttcgc
    42541 cgccgggctg gcacctgact gtgcaataac cgtacatacc caggcagata cgtccagtcc
    42601 cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg cgaacgtgac
    42661 tgacgcgatc ctcagcaggg ctggagggtc aattgctgac tttaccgggc atcggcaaac
    42721 ggcgtttcgc gaactggaac gggtgcttaa tttcccgcaa tcaaacttgt gccttaatcg
    42781 tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac tcaaggtgag
    42841 cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga cggaaatatt
    42901 tctcctgcaa caagcacagg gaatgccgga gccggggtgg ggaaggatca ctgattcaca
    42961 ccagtggaac acgttgctaa gtttgcataa cgcgcaattt tatttactac aacgcacgcc
    43021 agaggttgcc cgcagccgcg ccaccccgtt gttggatttg atcatgacag cgttgacgcc
    43081 ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtgc tgtttatcgc
    43141 cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact ggacgcttcc
    43201 aggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct ggcgtcggct
    43261 aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac agcagatgcg
    43321 tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga ccctggcagg
    43381 atgtgaagag cgaaatgcgc agggcatgtg ttcgttggcc ggttttacgc aaatcgtgaa
    43441 tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc agctacctga
    43501 atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga aaaggcgttc
    43561 acgccgcatc cggtcacttt accttcagtt ttactctttc tcggagtaac tataaccgta
    43621 atagttatag ccgtaactgt aagcggtgct ggcgcgttta atcacaccat tgaggatagc
    43681 gcctttaata ttgacgcctg cctgttccag acgctgcatt gacaaactca cctctttggc
    43741 ggtgttcagg ccaaaacgtg caaccagcag gctggtgccg acagagcgcc ccacgaccgc
    43801 agcatcgctc accgccagca tcggcggcgt atcgacaatc accagatcgt aatggtcgtt
    43861 cgcccattcc agtaattgac gcatccgatc gcgcatcagc agttcagacg ggttaggtgg
    43921 cacctgaccg cgagtaatca catcaaagcc tcctttgcca aaatgctgga tcactttgtt
    43981 gagctcatct ttacctgcca gatattccga caagccatgt tcattactca cggtaaacag
    44041 gttatgcgaa taaccacggc gtaagtcggc atcaataaat aacacttttt gatcggactg
    44101 ggcgatcacc gctgccagag ttgaactgac aaacgtttta ccactgtctg gcgtcgcacc
    44161 ggtgatcatc agaatgttat tctcagtctc catcatagcg aaatgcagac tggttcgtag
    44221 cgcacgtacg gcttccacag cagaatccgc cgggttatcc accgccagga aggggatatt
    44281 tttagtacga tggcgctgct gattagaaaa taaatttttc ttacgcagac gggtgcgttt
    44341 atccagccac tcggacattg ggatagtggc ataaacgctg atgccgtgct cttccagttg
    44401 ttccggtgct tctacaccac gacgcaacat cgcacgcgcc agcacggcac ccaccgaaat
    44461 aaacaggcca agaataaaac caagcaccac attcaacgct tttttcggtt tcactggctg
    44521 cggctgagtg actgccgggt cgataatccg cacgttacca atggcactgg atttcgaaat
    44581 actcaactcc tgctggcggt taagtaattg cagatatacc gcacggcccg cttctacgtc
    44641 acgacttaaa cgcaacactt cctgttgggt ggaaggcata gccgataccc gcttattcag
    44701 gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc agcgcacgat aagttgggtg
    44761 atctttctta tacagctggg agatctctgc ctcgcggaag gtcagctcat tgagttgatt
    44821 atcaacgttc acaatctgct caagaacggc tttggcttcc aggttaaggt caaccgaatc
    44881 gcgctgctgg cgataaacgt tgagtttttc ttccgcctgg tccagctcgc tgcgcacttc
    44941 tggcaactgg cgctgtaaga attcaaggct ttgtgaatcc tgcgccgcct ggcgagcgat
    45001 attctgttgc aaatagttgt tagcgatgct gttcagaata cgagtaatca actggggatc
    45061 atcaccagtc atggtaagtt ccagcatccc gctttcttta ctgcgttcgc taacggtaaa
    45121 ggtttcctgc aacgcgttaa tcgcttccag ttcggtacgc tggctcagga caaactgtgt
    45181 tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg ccatcttttt ccagacgctg
    45241 gccgaccata ccattgacgg tgaactcttc accttccagc gtatagtggc cgttttcccc
    45301 aaccgtgagt gtcagttgct gatcctgacc attcagttgt ggaatatgca tccagctgat
    45361 cgccagctca cctggttttt ctttggttaa tctcgcccag ccgcgaccca caatcggaaa
    45421 atacttctgc tcaaccatgt cgcgcagatt cagttcagca atggttttac cgagaatcat
    45481 gcgcgattgc agcagttgga tctccggtgc agactcgggc gatgagttag ggatcatatc
    45541 actcaggccg ctgagaatgg cgttgccctg tttttgctca acctggacca gagtatctgc
    45601 ctgataaatt ggtgtactta acagcgagta agcgacagcg atcagcgtga ataacgcggt
    45661 cacgctgata ataaacttac ggtgatccca taactcgccg accagacgaa gcagatcgat
    45721 ctcattttcc tgagtgctgc ctggtggcgt attcatattt ttagttgtca tcgtatttta
    45781 taaactcaaa ttaccggctg aggcgcttcg cccattcctg actggcgcgc tccaacatac
    45841 cgtagacatg ttcaaatgcg tcctgacttt tacgataggg atccgggatc tctttctgtt
    45901 ccagccattg cccaaacagc attgtcttcc cgcggacctc gggagcgatt gcggtaacct
    45961 gagcaatatg ttccgactcc attgccagaa tcagatcgta atttcgtgcc atctcagcag
    46021 tgagcttacg tccggcatgt ccttcaagag atacgccatg attagcggca acatctgctg
    46081 ctgtcgcatc tgcagggtgt tttaccagac catgaacacc tgccgatttg acttttacgc
    46141 ctggcagacg tttacgcagc aagcgctcgc caatgggcga acggcaaatg ttgccggtac
    46201 aaaccaccag gattgagtta aattttagtt gggccatgtt tgaatccacc gtgaagtctc
    46261 tgtcaggtca tgcaccccgg aaatcgtcgg taccagcagg gaaatcacac ggttccaacg
    46321 agccagcggc gcggtagtga cataaacaat gtcgtaaggt tcaagctgga attccgttcc
    46381 gagtatcatt gccgatgcgt ccttcgcatt cagctgataa atattggcga ttttgccatt
    46441 ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc gcatcagcga catcctgatt
    46501 cataccctca gcgttaccca gcgcttccgc cagggtcata ccgctgcggt ccattttcag
    46561 cgtgctctgt ttacccactt cacccatcac gaagactttc agatcatcgt tgcgcggaat
    46621 aaataaaata tcgcccgggt gcagcagttt gttctggcgc aagtcgccac gctgcatcag
    46681 cgcatagagg ttcactttgg tctttacgcc attctgggtg agcaccacgt tgcgccagtc
    46741 tgcatctgcg gtcagaccgc ccgcggcgtt aatcgcatcc atgatggtca gaggaatatt
    46801 ggtaatcggc tgctgacccg atttgctgac ttcgccggtg acgtacgctt tttgcgagcg
    46861 gaacgccgcg acgctgacat cgacttgcgg gctttcaatg actgaatcca gacgcgctgt
    46921 aatctcgttg cgtacctggg taagtgtttt cccggccact ttcaggcgac caatataggg
    46981 gtagaaaatc gcaccatcgg cgttaaccca gttgccggta tcactggcgc tacggtattg
    47041 ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta accatcaaca catcaccaat
    47101 gccgatacga tattcatagt tcgcgatttg ctgctccagc tccgggttag cctgcgacat
    47161 aatcggtttg gcgcgcaact gatcaatcaa tcctggcgtg acgggataga tattgaccat
    47221 tttgtccagg tcatactgat tgtcaggtag ttcaatgaca tccttgttac tggtcgataa
    47281 attctgacca ggaacaaggg tacatgctgt caaagacaga acagccaatg tcagtaccga
    47341 aaacttaaaa atgttctttt tcattggttt atatttatca gtcaatgtaa tgttatgaat
    47401 tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc gtcattggat agagctggta
    47461 tttgcgtgac agcatttgtc caccatcacg cgtcaatggt gtccacgaaa ccaccgcgcg
    47521 gttgcggtta ggtccaatgg tcatcaagtc gaacggaatc gagatataaa aacctttact
    47581 aaagccgcct tcgccgtagt catctttaga tacgttactg attgccgccc atacccctac
    47641 cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt gcgcctttat cttttgccag
    47701 atattgccca acgctaagtt tcatcagtac gccattgagc gtcggcgggt tccagtaagc
    47761 cgtcacgaaa ccagttggcg tggaataatc ggtgaagcgc atcatgttgt cccagtcacg
    47821 ctgcttcacg tagttaacgt ccacacccag cgcccagctg gcatctagcg ggcgataaag
    47881 cagctcggaa ccgacaccgg catacatcgt ttccagataa ccgccataca cctggccata
    47941 gaaaccattg cctaagtcgg caaagtaatt cgcttgcaag ttgttgagat aaacgtcatt
    48001 gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg gtagagtccg cgggcaacag
    48061 tgaagactta aacttgtcgt agttgttgta aatattggtg aaaataccgc catcaagcag
    48121 caggtggtcg gtaaaccagt agcgggcgct ggacatcagc cccatctgga acatatagaa
    48181 atcttccggg ccgcccagcg actgtgaaag tgttgggttg aaagagtagc taaagcgatc
    48241 ttcacgaata cggtagcccc gaccaaaggc agaaagatct tcagcttcaa cacgttgttg
    48301 ttgcagtggc tctgattgac caggcgctgt acctgccagc tgtttgcgca ggctggctac
    48361 gtcggtttcg gtagtcacca tcgccatatg ctcgcgcttt tgcgtcacgc taatcttctc
    48421 aacgccttgc ggcaggttat tcaccagaat ccggttggca cgatcgaccg cttcacgaga
    48481 gtcacggtat ttgtattgct gaccagacat atacagcgtc ttatcgcgta gctgaatttc
    48541 tggcgcgtca aagcccgcgt tatacttcag ggcggtaagt tggtttgcta ccgtggtgta
    48601 ctgcaatcct tcagattcag gcgcaggttg atatgccggt tttggcgtat cgcgcagggc
    48661 tggacgcaga tcgttgaaat tggtccgtaa agtgaaacca aacatcaacg tgttaccgcg
    48721 ttcataactc aggttgaggt ctgcccagct ggcagcgcga taaactgcac cgacgttgaa
    48781 atggcttgcc tgaggcagtt tgccagcgaa atcattctgg taattgttgc cgtcgtattc
    48841 gagtttcaga cgcaggggat tccacggcgt ttgatactca atgccgccaa agatggaggc
    48901 cgggccacga aagatatcgc taaagctgat atcgcccgca tcgtgggatt ctgcacgatg
    48961 acaatattta tcgctcaccc ggcaaaacgg gttggtaata ttgcccgcat taccggcgta
    49021 gccccatgcc atcccgaggg tgaaatcaaa tggccccgct tgcttgctgg ccaccagata
    49081 ctcaccgtca aacagacccg taccagcaat atcacgttta ccaaacgcca cttgcggtag
    49141 ccagtaacct tcttcccaca agcgaagttt aaaatcgaaa gatttgtctt tgtatgactg
    49201 atcgccgctg aaatcttccc actggctata tttacgtgtg cgcacatccg tataacgaat
    49261 ggtgccttcc agccatggga acagcgccac ggaggtggag tagaaacggt attgatcgtt
    49321 atcccggtag ttgacgctga attcaccttc cggtgcgatg cgcgcatttg gcatctgcaa
    49381 caatcctgtg ccgccaaagt ccgattgcga cgaacccagc ggatccgggt aggttaatac
    49441 ttcagcatga caggcactgg agacggcaat ggccaggcag cttaaaagat aagaattctt
    49501 cttcatcagt caggaacacg ttgcgttaaa acagacacaa tctggtcatt taaatcagca
    49561 tatttttcgg gcagtacatg cgcagaaaag cctaaccaaa gctggctgcc aggcggtggt
    49621 tccacatgtc ttttattcca caaggcaaca ggggcgacga cagtctcacc ctcaggcgta
    49681 atcaccataa cgttattctt gtccgcgcct gcaagacggg ggtgatcttg cagataatcg
    49741 gtaacgctac ggcctgcctg ccagggcaat tgcccagccc cagaaaccgc acccagcaga
    49801 gtaatagtca ccggacgctg tacggtatag agcgtatagt ccccgaccag cgggggattg
    49861 ctattctcat ccactcgtac aaaatcaggg tccagtttga ccggtaagcg accagtgata
    49921 ttgagattga gtaactgctg gcgcacggat ttaatcgttg ccgccacatc gtcgtcagct
    49981 tctgcttccc aggaagcaag ctgtgccatg acatgttggt agtctttaag cgctttagct
    50041 ttcaccgcgg agtcggtcag cagggcacca ggccaccaga ggcgatcgcg cagttgcggt
    50101 tgtgtcacca actgggcaac attctccacg gggccaacgg aaagtgtctg ttgttcgcca
    50161 ggcagataaa tagtcaccgt tccttgcgca aaggcatggg gtgtcattac gtaaagtacg
    50221 ctggcaatga aatacgactg taatttattc atggtgcggg tttaagaaac gtcatttcta
    50281 cgggaataac ccctgcgcct aacatttgcc gactttgacg aacctggcca gtagccgaat
    50341 cgacccagaa tgtgttttgc cactgttttt ctggtagcgt ggaagtcacc tcttcctgcc
    50401 agacattaca cagcacatcg cgacccgcaa tatgcagcgt ctctaatcca gcaaaggaaa
    50461 aagtggaact cagggtcgca gagcgaaaat ggttgtcttc actccagaga atatcgcgcg
    50521 tccagcggct gccgtctttt attgccagcg cgttacgcag agggtcctgc ccggaatttg
    50581 tcacttccag cagattatta ttaagtttga cggtctttaa aagacgtcca ttgtgggtaa
    50641 ccagcatggc gttatcctgg gataaccatt tgctttgttc ttgttctata tatcccagta
    50701 ccacaaagat tcgttgccct tcattaaggc gtaaatacat cgtggaataa ggcaacgcct
    50761 ggatctgctg atccgctacg gtgatatcct gattatcgaa aaggctggcg cgaaatgtat
    50821 cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa tagtgcgaaa atcgataaaa
    50881 taagagggcg cacctttaat ccttgaaagg ttaattaaaa aaatgcaaat ctctaaagcc
    50941 cggtacgaat atcgcaaccg ggtatcattt aactttaatt tcgacgcgtt aaagcagtat
    51001 tagcgtgtag tggacgtggt ggttgaggtg ttatgagaac cgccatcgcc actgtcattt
    51061 gctgcaacaa ctgccaacgt cgccacacca acagccgttg ccacaccgac aaccgtcgcc
    51121 gtgccgacag cgccagcagc cacgccacca gtggtagcag ccgtcgtggt tgcaccggta
    51181 gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg tcccgtttgc tgcttcaggg
    51241 gcagcaaacg cagctggcgt cgtcaacatg aaggccataa ggattgcaga aagcttgtgt
    51301 ttcataactt ttcctttatt catcgcatgg acaatacgta cgcttgagat caggattatc
    51361 tactttgagt ataacatcct tgtgaaatct ccctttcctc ataccgccag cactatatac
    51421 tgcgggtggg aaaggcgcgc agagggaaat ataagattgt taactattcc cctct
//
LOCUS       DAPGRN010000019        50576 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPGRN010000019 DAPGRN010000000
VERSION     DAPGRN010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.019"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(147..1511)
                     /gene="rhlE"
                     /locus_tag="RHW33_001246"
     CDS             complement(147..1511)
                     /gene="rhlE"
                     /locus_tag="RHW33_001246"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV1644770.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSSSAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1740..2411
                     /gene="cecR"
                     /locus_tag="RHW33_001247"
     CDS             1740..2411
                     /gene="cecR"
                     /locus_tag="RHW33_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV1644771.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2414..3409
                     /gene="hlyD"
                     /locus_tag="RHW33_001248"
     CDS             2414..3409
                     /gene="hlyD"
                     /locus_tag="RHW33_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV1644772.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLISPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3402..5138
                     /locus_tag="RHW33_001249"
     CDS             3402..5138
                     /locus_tag="RHW33_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1644773.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5131..6264
                     /locus_tag="RHW33_001250"
     CDS             5131..6264
                     /locus_tag="RHW33_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1644774.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6275..7381
                     /locus_tag="RHW33_001251"
     CDS             6275..7381
                     /locus_tag="RHW33_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1644775.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7343..7753)
                     /locus_tag="RHW33_001252"
     CDS             complement(7343..7753)
                     /locus_tag="RHW33_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV1644776.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLASKHRVRR"
     gene            7886..8647
                     /locus_tag="RHW33_001253"
     CDS             7886..8647
                     /locus_tag="RHW33_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV1644777.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8644..9885
                     /gene="clsB"
                     /locus_tag="RHW33_001254"
     CDS             8644..9885
                     /gene="clsB"
                     /locus_tag="RHW33_001254"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV1644778.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVAP
                     PAQPTMETQDRVETENTGVKP"
     gene            9885..10841
                     /locus_tag="RHW33_001255"
     CDS             9885..10841
                     /locus_tag="RHW33_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV1644779.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10877..11590)
                     /locus_tag="RHW33_001256"
     CDS             complement(10877..11590)
                     /locus_tag="RHW33_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV1644780.1"
                     /translation="MESYLQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINSAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAFIGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11796..12500)
                     /locus_tag="RHW33_001257"
     CDS             complement(11796..12500)
                     /locus_tag="RHW33_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV1644781.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12637..13089)
                     /gene="moaE"
                     /locus_tag="RHW33_001258"
     CDS             complement(12637..13089)
                     /gene="moaE"
                     /locus_tag="RHW33_001258"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV1644782.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13091..13336)
                     /gene="moaD"
                     /locus_tag="RHW33_001259"
     CDS             complement(13091..13336)
                     /gene="moaD"
                     /locus_tag="RHW33_001259"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV1644783.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13329..13814)
                     /gene="moaC"
                     /locus_tag="RHW33_001260"
     CDS             complement(13329..13814)
                     /gene="moaC"
                     /locus_tag="RHW33_001260"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV1644784.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13817..14329)
                     /gene="moaB"
                     /locus_tag="RHW33_001261"
     CDS             complement(13817..14329)
                     /gene="moaB"
                     /locus_tag="RHW33_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV1644785.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14351..15340)
                     /gene="moaA"
                     /locus_tag="RHW33_001262"
     CDS             complement(14351..15340)
                     /gene="moaA"
                     /locus_tag="RHW33_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV1644786.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15328..15472)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15737..16645
                     /gene="yvcK"
                     /locus_tag="RHW33_001263"
     CDS             15737..16645
                     /gene="yvcK"
                     /locus_tag="RHW33_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV1644787.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(16836..18857)
                     /gene="uvrB"
                     /locus_tag="RHW33_001264"
     CDS             complement(16836..18857)
                     /gene="uvrB"
                     /locus_tag="RHW33_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV1644788.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19436..20113)
                     /gene="bioD"
                     /locus_tag="RHW33_001265"
     CDS             complement(19436..20113)
                     /gene="bioD"
                     /locus_tag="RHW33_001265"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV1644789.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESSVMSSG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YIDLALL"
     gene            complement(20106..20861)
                     /gene="bioC"
                     /locus_tag="RHW33_001266"
     CDS             complement(20106..20861)
                     /gene="bioC"
                     /locus_tag="RHW33_001266"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV1644790.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYRHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDQRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIVRE"
     gene            complement(20848..22002)
                     /gene="bioF"
                     /locus_tag="RHW33_001267"
     CDS             complement(20848..22002)
                     /gene="bioF"
                     /locus_tag="RHW33_001267"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV1644791.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     TCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNGRALQL
                     AEKLRQQGCWVTAIRPPTVPSGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21999..23039)
                     /gene="bioB"
                     /locus_tag="RHW33_001268"
     CDS             complement(21999..23039)
                     /gene="bioB"
                     /locus_tag="RHW33_001268"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV1644792.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23126..24415
                     /gene="bioA"
                     /locus_tag="RHW33_001269"
     CDS             23126..24415
                     /gene="bioA"
                     /locus_tag="RHW33_001269"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV1644793.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LVCVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24474..24950
                     /locus_tag="RHW33_001270"
     CDS             24474..24950
                     /locus_tag="RHW33_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV1644794.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSDLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25102..26385
                     /locus_tag="RHW33_001271"
     CDS             25102..26385
                     /locus_tag="RHW33_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV1644795.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPTQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTVKPWGDAVISNRPFAGNTGSVD
                     DSDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(26526..28787)
                     /locus_tag="RHW33_001272"
     CDS             complement(26526..28787)
                     /locus_tag="RHW33_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV1644796.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGQIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(28970..30403)
                     /locus_tag="RHW33_001273"
     CDS             complement(28970..30403)
                     /locus_tag="RHW33_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV1644797.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(30479..31531)
                     /locus_tag="RHW33_001274"
     CDS             complement(30479..31531)
                     /locus_tag="RHW33_001274"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV1644798.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIIPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            31715..32668
                     /locus_tag="RHW33_001275"
     CDS             31715..32668
                     /locus_tag="RHW33_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1644799.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFS
                     PLVNEPFVLACRRDHPLAKKQLVEWLELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(32709..33704)
                     /gene="pgl"
                     /locus_tag="RHW33_001276"
     CDS             complement(32709..33704)
                     /gene="pgl"
                     /locus_tag="RHW33_001276"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV1644800.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVVQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            33859..34677
                     /gene="ybhA"
                     /locus_tag="RHW33_001277"
     CDS             33859..34677
                     /gene="ybhA"
                     /locus_tag="RHW33_001277"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV1644801.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(34678..35736)
                     /gene="modC"
                     /locus_tag="RHW33_001278"
     CDS             complement(34678..35736)
                     /gene="modC"
                     /locus_tag="RHW33_001278"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV1644802.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(35739..36428)
                     /gene="modB"
                     /locus_tag="RHW33_001279"
     CDS             complement(35739..36428)
                     /gene="modB"
                     /locus_tag="RHW33_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV1644803.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(36428..37201)
                     /gene="modA"
                     /locus_tag="RHW33_001280"
     CDS             complement(36428..37201)
                     /gene="modA"
                     /locus_tag="RHW33_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1644804.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPFAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(37368..37517)
                     /gene="acrZ"
                     /locus_tag="RHW33_001281"
     CDS             complement(37368..37517)
                     /gene="acrZ"
                     /locus_tag="RHW33_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV1644805.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            37646..38434
                     /gene="modE"
                     /locus_tag="RHW33_001282"
     CDS             37646..38434
                     /gene="modE"
                     /locus_tag="RHW33_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV1644806.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            38502..39971
                     /gene="modF"
                     /locus_tag="RHW33_001283"
     CDS             38502..39971
                     /gene="modF"
                     /locus_tag="RHW33_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV1644807.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITHLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQVLMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKI"
     gene            40236..41252
                     /gene="galE"
                     /locus_tag="RHW33_001284"
     CDS             40236..41252
                     /gene="galE"
                     /locus_tag="RHW33_001284"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV1644808.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            41262..42308
                     /gene="galT"
                     /locus_tag="RHW33_001285"
     CDS             41262..42308
                     /gene="galT"
                     /locus_tag="RHW33_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV1644809.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            42312..43460
                     /gene="galK"
                     /locus_tag="RHW33_001286"
     CDS             42312..43460
                     /gene="galK"
                     /locus_tag="RHW33_001286"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV1644810.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            43454..44494
                     /gene="galM"
                     /locus_tag="RHW33_001287"
     CDS             43454..44494
                     /gene="galM"
                     /locus_tag="RHW33_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV1644811.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLLPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPRDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGNGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            44697..45449
                     /gene="gpmA"
                     /locus_tag="RHW33_001288"
     CDS             44697..45449
                     /gene="gpmA"
                     /locus_tag="RHW33_001288"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV1644812.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(45615..46667)
                     /gene="aroG"
                     /locus_tag="RHW33_001289"
     CDS             complement(45615..46667)
                     /gene="aroG"
                     /locus_tag="RHW33_001289"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV1644813.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            46983..47363
                     /locus_tag="RHW33_001290"
     CDS             46983..47363
                     /locus_tag="RHW33_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV1644814.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            47477..48418
                     /gene="zitB"
                     /locus_tag="RHW33_001291"
     CDS             47477..48418
                     /gene="zitB"
                     /locus_tag="RHW33_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV1644815.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(48415..49134)
                     /gene="pnuC"
                     /locus_tag="RHW33_001292"
     CDS             complement(48415..49134)
                     /gene="pnuC"
                     /locus_tag="RHW33_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV1644816.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALTWLAVCVIAIGLMTVFINPVFAFLTRVAVIIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(49172..50215)
                     /gene="nadA"
                     /locus_tag="RHW33_001293"
     CDS             complement(49172..50215)
                     /gene="nadA"
                     /locus_tag="RHW33_001293"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV1644817.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(50493..>50576)
                     /locus_tag="RHW33_001294"
     ncRNA           complement(50493..>50576)
                     /ncRNA_class="other"
                     /locus_tag="RHW33_001294"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT        12244 a        13406 c        12909 g        12017 t
ORIGIN      
        1 aataatttca ttgccatttt agatacacta gatagatcat aaagcaaatt tattaaaaca
       61 ataaaaacag acatgtcatg catgtctgac cgtgttacct gaataatggg caaaagcctg
      121 tgatactctt ttgcccttac atggaattac tgcgcagcgg caggtttacg cggacggcgg
      181 cgacgttgtt gttcgcctgc cggtttggca tcgccgaggc ggcgagacgg tttttctgca
      241 ggttttgcgc ttgaagattt cgcaccacct tctccacggc gtggttgctg ttgaccgcga
      301 ccaccacctt gcccacgacc gccgccgcca cgttgctggc gaccgttctg gatcggttcg
      361 gctttgattg acgggtccgg ctcatagccc ggaatcgcaa tgcgcgggat ctcttttttc
      421 agcagttttt cgatatcacg cagcagtttg tgttcatcaa cacacaccaa cgacaacgct
      481 tctccggtgg ctgcggcacg accggtacgc ccgatacggt ggacataatc ttccggtacg
      541 tttggcagtt catagttgac gacgtgcggc aactcttcga tatccagacc gcgcgcagcg
      601 atgtcagttg caaccagtac acgaatatcg cccgatttaa aatcggccag cgcacgggta
      661 cgcgcacctt gcgatttatt accgtggatc gccgcactac ggatgccatc tttattaagc
      721 tgttcagcga gatggttagc gccatgtttg gtacgggtaa acaccagcac ctgctgccag
      781 ttccctttcc caatcatgtg cgacagcaat tcgcgtttgc gtttcttatc aacaaagtga
      841 acatgctggg tcacctgatc agacgcggta ttgcggcgtg ccacttcgat ttccagcggg
      901 ttgtgcaaca gtttttccgc cagggcttta atatcgtcag agaaggtcgc ggagaacaac
      961 aggttctggc gcttcgcagg tagttttgtt aacacgcgac ggatatcgtg gataaagccc
     1021 atgtcgagca tgcggtcagc ttcatcgagg acgaggattt caacctgatc cagcttcact
     1081 gcattctgat gttccaggtc cagcaaacgt cccggcgttg ccaccagcac atcaacgccg
     1141 ccgcgcaact tcatcatttg tgggttaatg ctgacaccgc caaacaccac cagcgaacga
     1201 atgttcaggt atttgctgta atcacggacg ttttcgccaa tctgcgcagc cagttcacgg
     1261 gtcggggtaa gaatgagcgc acgtaccgga cgacgccctt tggcgtgcgg ctggcgagtg
     1321 atcaggtgtt gcaacagcgg cagcgtaaag cccgctgttt tgccggtgcc ggtctgggcg
     1381 ctagccatca ggtcgcggcc ttccagcacc gcagggatcg cctgctgctg aataggggtg
     1441 ggttcacggt aaccctgctc ggcaacggcg cgcaggatat cagggcttaa acccaaagaa
     1501 tcgaaagaca taactactcc gaaccgcccc gaccgttacc ggtgtagttt tcagggagat
     1561 actgaaaagg aaaatgacaa aaaccacaat gtcatgaggg agcggagtgt agcagttttt
     1621 gtgacgcagc gcataaatta tccctgtgac tggcgtagct aaaattctta gccatactgg
     1681 acaactccca tatggagtca taatcttaat caatcatttg attaagaatg gactgtgcga
     1741 tgaataatcc tgccatgaca atcaagggtg aacaggcgaa aaaacagctg attgctgccg
     1801 cactggcgca gtttggtgaa tatggaatga acgccaccac ccgcgagata gccgcccagg
     1861 ccgggcagaa tatcgctgcc atcacctact acttcggttc gaaagaagat ttgtacctcg
     1921 cctgcgccca gtggattgcc gattttattg gcgagcagtt ccgtccgcat gccgaggaag
     1981 ccgaacgctt gttcgcgcaa ccacagcctg atcgggctgc catccgtgaa ctgatccttc
     2041 gcgcctgcag gaacatgatt aagctgctca cccaggatga caccgtcaac ctcagcaagt
     2101 ttatctcccg tgagcagctc tctcccacgg cagcctacca cctggtgcac gaacaggtga
     2161 ttagtccgct acacagccac ctgacacgcc tgattgccgc ctggaccggc tgcgacgcca
     2221 atgacacccg catgatcctt catacccatg cgttgattgg tgagattctg gcgttccgtc
     2281 ttggcaaaga aacaattctg ttacgtaccg gctggaccgc gttcgatgaa gaaaagaccg
     2341 aactgatcaa ccagacggtg acctgtcata tcgacctgat tttgcaagga ttatcgcaaa
     2401 ggagtttgta gtgatgaaaa aacctgtcgt gatcggactg gcggtagtgg tacttgccgc
     2461 cgtggttgcc ggaggctact ggtggtatca aagccgccag gataacggcc tgacgctgta
     2521 tggcaacgtg gatattcgta cggtgaatct tagtttccgt gttggggggc gcgttgaatc
     2581 gctggcggtg gacgaaggtg atgcgatcaa agcgggccag gtgctgggcg aactggatca
     2641 caagccgtat gagattgccc tgatgcaggc gaaagcgggc gtctcggtgg cacaggcgca
     2701 gtatgacctg atgcttgccg ggtatcgcga tgaagaaatc gctcaggccg ccgctgcggt
     2761 gaaacaggcg caagccgcct atgactatgc gcagaacttc tataaccgcc agcaagggtt
     2821 gtggaaaagc cgcaccattt cggcaaatga cctggaaaat gcccgctcct cgcgcgacca
     2881 ggcgcaggca acgctgaaat cagcacagga taaattgcgt cagtaccgtt ccggtaaccg
     2941 tgaacaggac atcgcccagg cgaaagccag cctcgaacag gcgcaggcgc aactggcgca
     3001 ggcggagctg aatttacagg actcaacgtt gatatcaccg tctgatggca cgctgttaac
     3061 gcgcgcggtg gagccaggca cggttctcaa tgaaggtggc acggtgttta ccgtttcact
     3121 aacgcgtccg gtgtgggtgc gtgcttatgt tgatgaacgg aatcttgacc aggcccagcc
     3181 ggggcgcaaa gtgctgcttt ataccgatgg tcgcccggac aagccctatc acgggcagat
     3241 tggtttcgtt tcgccgactg ctgaatttac cccgaaaacc gtcgaaacac cggatctgcg
     3301 taccgacctc gtctatcgcc tgcgtattgt ggtgaccgac gccgatgatg cgttacgcca
     3361 gggaatgcca gtgacggtac aattcggtga cgaggcagga catgaatgat gccgttatca
     3421 cgctgaacgg cctggaaaaa cgctttccgg gcatggacaa gcccgccgtc gcgccgctcg
     3481 attgtaccat tcacgccggt tatgtgacgg ggttggtggg gccggacggt gcaggtaaaa
     3541 ccacgctgat gcggatgttg gcgggattac tgaaacccga cagcggcaat gccacggtga
     3601 ttggctttga tccgatcaaa aacgacggcg cgctgcacgc cgtgctcggc tatatgccgc
     3661 agaaatttgg tctgtatgaa gatctgacgg tgatggagaa cctcaatctg tacgcggatt
     3721 tgcgcagcgt caccggcgag gcacgtaagc aaacttttgc tcgcctgctg gagtttacgt
     3781 ctcttgggcc gtttaccgga cgcctggcgg gcaagctctc cggtgggatg aaacaaaaac
     3841 tcggcctggc ctgtaccctg gtgggcgaac cgaaagtgtt gctgctcgat gaacccggcg
     3901 tcggcgttga ccctatctca cggcgcgaac tgtggcagat ggtgcatgag ctggcgggcg
     3961 aagggatgtt aatcctctgg agtacctcgt atctcgacga agccgagcag tgccgtgacg
     4021 tgttactgat gaacgaaggc gagttgctgt atcagggaga accaaaagcc ctgacacaaa
     4081 ccatggccgg acgcagcttt ctgatgacca gtccacacga gggcaaccgc aaactgttgc
     4141 aacgcgcctt gaaactgccg caggtcagcg acggcatgat tcaggggaaa tcggtacgtc
     4201 tgatcctcaa aaaagaggcc acaccagacg atattcgcca tgccgacggg atgccggaaa
     4261 tcaacatcaa cgaaactacg ccgcgttttg aagatgcgtt tattgatttg ctgggcggtg
     4321 ccggaacctc agaatcgccg ctgggtgcaa tattgcatac ggtagaaggc acacccggcg
     4381 agacggtgat cgaagcgaaa gaactgacca agaaatttgg tgattttgcc gccaccgatc
     4441 acgtcaactt tgccgttaaa cgtggggaga tttttggttt gctggggcca aacggcgcgg
     4501 gcaaatcgac cacctttaag atgatgtgcg gtttgctggt gccgacttcc ggccaggcgc
     4561 tggtgctggg gatggatctg aaagagagtt ccggtaaagc gcgccagcat ctaggctata
     4621 tggcgcaaaa attttcgctc tacggcaacc tgacggtcga acagaattta cgctttttct
     4681 ctggtgtata tggcttacgc ggtcgggcgc agaacgaaaa aatctcccgt atgagcgagg
     4741 catttggcct gaaaagtatt gcctcacacg ccaccgatga actgccatta ggttttaaac
     4801 agcggctggc gctggcctgt tcgctgatgc atgaaccgga tattctgttt ctcgacgaac
     4861 cgacttccgg cgttgacccc ctcacccgcc gtgaattctg gctgcacatc aacagcatgg
     4921 tagagaaagg cgtcacggtg atggtcacca cccactttat ggatgaagcg gaatattgcg
     4981 accgcatcgg cctggtgtac cgcgggaaat taatcgccag cggcacgccg gacgatttga
     5041 aagcacagtc ggctaacgat gagcaacccg atcccaccat ggagcaagcc tttattcagt
     5101 tgatccacga ctgggataag gagcatagca atgagtaacc cgatcctttc ctggcgtcgc
     5161 gtgcgggcgc tgtgcgtcaa agagacgcgg cagatcgttc gcgatccgag tagctggctg
     5221 attgcggtag tgatcccgct gttgctgctg tttatttttg gttacggcat taacctcgac
     5281 tccagcaagc tgcgggtcgg gattttactg gaacagcgta gcgaagcggc gctggatttc
     5341 acccacacca tgaccggttc gccctacatc gacgccacca tcagtgataa ccgacaggaa
     5401 ctgatcgcca aaatgcaggc ggggaaaatt cgcggtctgg tggttattcc ggtggatttc
     5461 gccgaacaga tggagcgcgc caacgccacc gcaccgattc aggtgatcac cgacggcagt
     5521 gagccgaata ccgctaactt tgtgcagggg tatgtcgaag ggatctggca gatctggcaa
     5581 atgcagcgag cggaggacaa tgggcagact tttgaaccgc ttattgatgt acaaacccgc
     5641 tactggttta acccggcggc gattagccag cactttatta tccccggtgc ggtgaccatc
     5701 atcatgacgg tcatcggcgc gattctcacc tcgctggtgg tggcgcgaga atgggaacgc
     5761 ggcactatgg aggctctgct ctctacggag attacccgca cggaactgct gctgtgtaag
     5821 ctgatcccct attactttct cgggatgctg gcgatgctgc tgtgtatgct ggtgtcagtg
     5881 tttattctcg gcgtgccgta tcgcgggtcg ctgctgattc tgttttttat ctccagcctg
     5941 tttttactca gtaccctggg gatggggctg ctgatttcca cgattacccg caaccagttc
     6001 aatgccgctc aggtcgccct gaacgcggct tttctgccgt cgattatgct ttccggcttt
     6061 atttttcaga tcgacagtat gcccgcggtg atccgcgcgg tgacgtacat tattcccgct
     6121 cgttatttcg tcagcaccct gcaaagcctg ttcctcgccg ggaatattcc agtggtgctg
     6181 gtggtaaacg tgctgttttt gatcgcttcg gcggtgatgt ttatcggcct gacgtggctg
     6241 aaaaccaaac gtcggctgga ttagggagaa gagcatgttt catcgcttat ggacgttaat
     6301 ccgcaaagag ttgcagtcgt tgctgcgcga accacaaacc cgcgcgattc tgattttacc
     6361 cgtgctaatt caggtgatcc tgttcccgtt cgccgccacg ctggaagtga ctaacgccac
     6421 catcgccatc tacgatgaag ataacggcga gcattcggtg gaacttactc aacgttttgc
     6481 ccgcgccagc gcctttacac atgtgctgct gctgaaaagc ccacaggaga tccgcccgac
     6541 catcgacaca caaaaggcgt tactactggt gcgtttcccg gctgacttct cgcgcaaact
     6601 ggataccttc cagaccgcgc cgttgcagtt gatcctcgac gggcgtaact ccaacagtgc
     6661 gcaaattgcc gccaactacc tgcaacagat cgtcaaaaat tatcagcagg aactgctgga
     6721 aggaaaaccg aaacccaaca acagcgagct ggtggtacgc aactggtata acccgaatct
     6781 cgactacaaa tggtttgtgg tgccgtcgct gatcgccatg atcaccacta tcggcgtaat
     6841 gatcgtcact tcactttccg tcgcccgcga acgtgaacaa ggtacgctcg atcagctact
     6901 ggtttcgccg ctcaccacct ggcagatctt catcggcaag gccgtaccgg cattaattgt
     6961 cgccaccttc caggccacca ttgtgctggc gattggtatc tgggcgtatc aaatcccctt
     7021 cgccggatcg ctggcgctgt tctactttac gatggtgatt tatggtttat cgctggtggg
     7081 attcggtctg ttgatttcat cactctgttc aacacaacag caggcgttta tcggcgtgtt
     7141 tgtttttatg atgcccgcca ttctcctttc cggttacgtt tctccggtgg aaaacatgcc
     7201 agtatggctg caaaacctga cgtggattaa ccctattcgc cactttacgg acattaccaa
     7261 gcagatttat ttgaaggatg cgagtctgga tattgtgtgg aatagtttgt ggccgctact
     7321 ggtgataacg gccacgacag ggtcagcggc gtacgcgatg tttagacgca aggtgatgta
     7381 attttttatc tttcgccagc aaagacacta ccgccgggcc ggcaaggatt gccagccctg
     7441 caattgccag caaaaagttg ttttccagta cgcgggacaa cagccacagc acaatcagcg
     7501 ctgcgccaaa ataccaggtc gtggtcagct cttccagcac gtctgccacc tcacgccagc
     7561 gttgctcggg gtggcgctga aacaccagca tcatcactac cgtgacacaa tacagtgcgc
     7621 ataatcccac gccgacgtga accagagtct tactcatcca gcccaccacc agcagcgcca
     7681 ctaccagtaa atgcaacata atctgccagc aacttagacc cgttgcgaca cgaacacgtt
     7741 gttgccactt catggcttct ctcctgaaga atttatctct acttactcag agtaccgaac
     7801 tttacggaaa attccgtaag accgcacctt tttgtgacgc cgactacact atttcaatga
     7861 gataagaggc aaagacagga aaattatgcc cgatcaaaca caacaatttt cgttcaaggt
     7921 gctcaccatc aatattcaca aaggctttac cgcgtttaac cgacgcttca ttttgccgga
     7981 acttcgcgat gccgtgcgta ccgtcagcgc cgatattgtt tgcctgcagg aagtgatggg
     8041 cgcgcacgaa gttcatccgc tgcatgtgga aaactggccc gatacctcgc actacgagtt
     8101 tctcgccgac actatgtgga gcgattttgc ctacggtcgc aatgccgtat acccggaagg
     8161 gcatcacggc aacgccgtac tgtcgcgtta tcccattgaa cattatgaga atcgcgatgt
     8221 ttcggtcgat ggtgcggaaa agcgcggcgt gctctactgc cgcattgtgc cgccgatgac
     8281 cggaaaagcg attcatgtga tgtgcgtaca tctgggcctg cgtgaggcgc accgtcaggc
     8341 gcagcttgcg atgctcgccg aatgggtgaa tgagctaccg gacggcgaac cggtattggt
     8401 ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat ccgttaaaag tgcaggccgg
     8461 actggatgag atttttaccc gcgcccacgg acgcccggcg cgcacgtttc cggtgcaatt
     8521 tcctctacta cgactggaca ggatctacgt caaaaatgcc agcgccagcg cgccaaccgc
     8581 gttgccgctg cggacatggc gacacctttc tgatcatgcc cctttaagtg cggagattca
     8641 tttatgaaat gtagctggcg cgaaggcaat aagatccagt tgctggaaaa cggcgagcaa
     8701 tattatcccg cggtgtttaa ggcgattggc gaggcacaag aacgcatcat tcttgaaacg
     8761 tttatctggt ttgaggatga cgtcggcaaa caactgcatg cggcactact ggcagcagcg
     8821 caacgcgggg ttaaagcgga agtcttgctg gatggctacg gttcgccgga tctcagcgat
     8881 gagtttgtca atgaactgac ggcagctggc gtagtgttcc gctactacga tccccgccct
     8941 cgcctttttg gtatgcgcac caatgtgttt cgccggatgc atcgcaaaat tgtggtgatc
     9001 gacgcgcgta tagcctttat tggcgggctg aattactccg ccgagcatat gtccagctac
     9061 ggtccagagg ctaaacagga ttacgcggta cgccttgaag ggccgattgt cgaagatatt
     9121 ctccagtttg agctggaaaa cctgcctgga cagagcgcgg cacgacgctg gtggcgacgt
     9181 catcacaaag cggaagagaa ccgccagccg ggagaagcgc aggtattgct ggtctggcgc
     9241 gataacgaag aacatcgcga tgatatcgaa cgtcactatc tgaaaatgct cactcaggcg
     9301 cggcgggaag tgattatcgc caacgcttac ttcttccccg gctatcgatt tttacacgcc
     9361 ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga tcattcaggg cgaaccggat
     9421 atgccgattg tcagagtcgg cgcgcgcttg ctgtataact atctggttaa aggcggcgtt
     9481 caggtatttg agtaccgccg ccgcccgctc cacggcaaag tggcattgat ggacgatcac
     9541 tgggcgacag tagggtccag taatctcgat ccgctcagtt tgtcactgaa tctcgaagca
     9601 aatgtcatca tccacgatcg tcattttaac cagacgctgc gcgataatct gaacggcatt
     9661 atcgccgcag attgtcagca ggtggatgaa accatgctgc ccaaacgcac ctggtggaac
     9721 ctgaccaaaa gcgtgctggc gttccacttt ttacgccact tcccggcact ggttggctgg
     9781 cttccggcac acacaccacg tctggcgcag gttgctcccc ccgcacaacc gacaatggaa
     9841 acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac cctgatgagt aaatcacacc
     9901 cgcgctggcg cttagcaaag aagatcctca cctggctgtt ttttatcgcg gtgattgtgt
     9961 tgctggtggt ctacgccaaa aaagtggact gggaagaggt ctggaaggtc atccgcgact
    10021 acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt cgtcagctat ctgatttacg
    10081 gctgctatga cctgctcgcc cgtttctact gcggtcacaa actggcgaag cgccaggtga
    10141 tgctggtgtc gtttatctgc tacgccttca acctgacact cagtacctgg gtcggcggca
    10201 ttggtatgcg ctatcgtttg tactctcgtc tggggttgcc cggtagcact attacgcgga
    10261 ttttctcgct cagtattacc accaactggc tgggttatat tttgctggcg gggattatct
    10321 ttaccgcagg cgtggtggag ctaccggatc actggtatgt cgatcaaacc acgctgcgca
    10381 ttctcggcat tggcttactg atgattatcg cggtttattt gtggttttgc gcttttgcga
    10441 aacaccgcca tatgaccatt aaagggcaaa agctggtgct gccttcgtgg aaattcgccc
    10501 tcgcccagat gctgatttcc agcgttaact ggatggtaat gggagcgata atctggctgt
    10561 tacttggtca aagcgttaac tatttctttg tactgggcgt gttgctggtt agtagtattg
    10621 caggcgtcat cgtgcatatt ccggcgggga ttggtgtgct ggaagcggta tttatcgcat
    10681 tactggcagg ggagcataca tccaaaggca caattatcgc cgccctgctc gcttatcgcg
    10741 tcctgtatta ctttatcccg ctgctgctgg cgctgatctg ttacctgcta ctggaaagcc
    10801 aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg aagttcaggt aagttcttta
    10861 gtatgtgcat ttacgactaa tgaaaaaaac gcgtatgcct ttgccagaca agcgttatag
    10921 ctatcgcggc aagtatccca aagaagccga taaaatagta atagagtgtt aatgcaccta
    10981 taaaagcgca acgaaccgga ctccacaact cttcactatg gcatttcccc gccagttcaa
    11041 ggagcttatt tcgtccatgc gagataatgc ctgaccataa cacaatcatt aagcaactta
    11101 ttacccaaat gggccgttca ctcattaatg ccgcattcac gatgattacc agggccaacc
    11161 ctgtcaacgt catcataatg ataaaacggt gagaaccagg atcaacatta aataataaac
    11221 cggccagcat actgattaaa aacatcgcgc ccgcagtgct gaacacgatg ataatggcat
    11281 atacaggaac taaacttgca acgacaatcg ccaccaaaga gagtaaaaac agatatatta
    11341 cgcctgcgat ggcggaattt atcttgttga tatccttact taagccatac atacaaagta
    11401 ataataaccc tatggcgaca tacattggtg gtgaatcata tttggttgcc gtaatttcga
    11461 gatacttagc accccaggcc agccctgcgg ttgccaccaa acctaaagcg gtaatgagcc
    11521 atataccgtt taatatcctg gtctcgtgct gaaaatctaa tttattgctg ttttgcaagt
    11581 atgattccat tcttttagtt gtccttatag aaaaatatta atattcatca tgaataaatt
    11641 taaacccacg catacaaaat acttttgtat ttatggatac ggaaaacgct tccgctcggc
    11701 cataaggcat attttatacc tggtacttgt taagtgtcgt ttttagtaag aaaaaaaatc
    11761 ccggcaccag gccgggatca gcaggaagaa gagaattaac gacgattgcc aaaaattcgc
    11821 agcaacatca ggaacaggtt gatgaagtcc agatacaagg ttaacgcgcc aagaatggaa
    11881 tatttgcgca ggttcgacgt gtcgcgagta tcaatctgct cacccatatt tttcaatttc
    11941 tgagtgtcat acgccgtcag tccgacaaag acaatcacgc cgatgtaggt aactgcccac
    12001 atcaatgctt cgcttttcag ccagaagttg accagcgatg ccagcacaat gccgattaac
    12061 gccataaaca gcatattgcc aaagccactt aaatcgcgct tcgtggtgta accgtacagg
    12121 ctcattgcgc cgaacatccc ggcagtaacg acgaaggtac tggcgataga agcagcggta
    12181 taaacaatga aaatactgga aagcgtaaga cccgtcagcg ccgaataaag cataaagagc
    12241 atcgtcgtta cacctgcgct cagcttttga atcatcgctg ataacacaat aaccaatgct
    12301 aattgcgcga tgatcagacc gattaaaaag acacggttag tgaacaacag ctccatcacg
    12361 gccgcggaat tagccgcata ccaggcaaca aatgcggtca gcaacaagcc aacggtcatc
    12421 cagccataga cttgcgccat ataagtctgc aagccagccc ggggttgtac aattgaatca
    12481 gaacgtggga atctgtccat gacgatctcc tgaagatata aggaatatct taaggatact
    12541 gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca tgtactgagc ggcaaaattg
    12601 ccggatgcag cggtgcctta tccggctaac aaaaaactac cagcgttttg ctgcctgctg
    12661 atcgctctcc cgagcttcga cccagcggtc gccttcaggc gtggcttcgc gcttccagaa
    12721 cggtgcgcgg gttttgagat aatccataat aaactgcccg gcttcaaacg cactgctgcg
    12781 atgcgcactg gtgacaccga caaaaacgat ttcatcgccc ggccataatt ccccgatgcg
    12841 gtgaatcacc gtgacgcgcc ccagcggcca gcggttacgc gcttcatcga caatttctgc
    12901 cagtgctttt tcagtcatcc ccggatagtg ttcgagggtt aatgctttga cgctgtcgcc
    12961 caggttatgg ttgcgcacct taccggtaaa ggtgactacc gcaccgtctt cgtcacgctc
    13021 cgccagccac gggtactctt ctcctacgct gaacggctgc ggaccaacaa caattttggt
    13081 ttctgccatc ttaacctccg gttaccggcg ggaagaaagc tacttcgtcg ccgtcagtca
    13141 gcggatggtc aaaactcacc agcgtctggt tgacggcagc cagtaatttg ccatcttcca
    13201 gcgccaacgc ccagcgatcg ctctgcgcag ccaggtgctg gcgtaacgct tcaacggttg
    13261 ggaaatccgc agccacttcg gttgcatctg ttcctaccaa ctcgcgcacc tgggcgaaaa
    13321 aaagaacttt aatcatccgc ttccacctta aagtcacccg acttgccgcc gctcttcgcc
    13381 agcaaacgta ccggaccaat caccatatct ttttgcaccg ctttgcacat gtcataaatg
    13441 gtcagcgccg ccacggaggc cgcggttaac gcttccattt cgacaccggt tttcccggtc
    13501 aggcggcata aggtttctat acgtacccga ttgtgctccg gctcggcctg taaattgact
    13561 tcaactttgc tgagcatcag tgggtgacag agcgggatca gatcccaggt gcgttttgcc
    13621 gcctgaatac cggcaatacg cgcagtggca aatacgtcgc ctttgtggtg gcgaccatca
    13681 ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg cttccgcccg cgcttcacgc
    13741 acggtttccg ctttggcgga gacatccacc atgtgcgctt cgccagcggc gttgatatgg
    13801 gtcagttgcg acatacttat ttcttcaaat gtggatggaa attacacgga cgcgtacggg
    13861 catccagctg cggcgcgatg atattttccc atgcggtacg gcacgctttg gtcgaacccg
    13921 gcatggcgaa aatcagcgtt ttgttagcga cgcccgctac cgcacgagat tgcaacgtgg
    13981 aagtgccaat ctcttcgaac gacaacatcc ggaacacttc accaaaacct tcaacttcac
    14041 ggtcgaacaa cggcagcagt gcttcgggag cctgatcacc ttccgtcagg ccagtccccc
    14101 ccgtaatcaa caccacttgt acatcgtcgc tggcgatcca cgcagatacc tgagcgcgaa
    14161 tagcgtagcg gttttctttc acaatggctt tatcgacaac gtgatggccc gcttcttgcg
    14221 ccgaatcgcg caaatagtga ccggaggtat cgtcttcttc accgcgacga ttagaaaccg
    14281 taagaatagc aatacgggtc gggataaatt cagtgcttac ctgactcatc tgatctctcc
    14341 ttttgacgtt ttagccgcca atgtacgata agttttgcgt aataccggtg ttgttttgat
    14401 gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga aatacgcgct tccagcgcct
    14461 gttgctgggt atcgtcttcc agcagatcgc gcaggttaac gccgccttca ccaaacaggc
    14521 agagatggag tttaccaatg gaggaaacgc gcaggcggtt gcaagtggcg cagaagtctt
    14581 tttcatacgg catgataagg ccaatctctc cggcgtaatc cggatgacaa aagacttgcg
    14641 cgggaccgtc gctgcgttga cgtaattggt ggatccagcc gcgacgcagt agctcgtcac
    14701 gcagaacctg accagagatg tgatgcttac ggaaaagctc gctgccctcg cccgtttcca
    14761 tcagttcgat gaaacgcagc tggatagggc gatgctggat ccagttcaga aaggtgtcga
    14821 gctggtgatg attcacatca cgcatcagca cggtattgac tttgaccttc tcaaaaccag
    14881 cctcaaatgc agcatcgatc cctgccatca cctgattgaa tttatcctgg ccggtaatgg
    14941 catgaaactg gcgggcgtcc agactgtcga cgctgacgtt gatgccagta agtccagcat
    15001 cgcgccagtt cgccacatcg cgttccagac ggtaaccatt ggtggtgaca gcaatctggc
    15061 ggatagcgtc gttttctcgc acagcggcga tgatatcggt aaagtcgcgg cgtaaagacg
    15121 gctcaccgcc tgtcagacgc actttttcgg tgcccagact ggcgaaggcg cgcgtaaccc
    15181 ggcgaatttc atcgacggtc agaaagcctt tattggtgac gccgctcggt ttgtagccat
    15241 ccggcaggca gtaggtgcaa cgaaagttac acacatcggt aatcgacagg cgcaagtagt
    15301 aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat gtacaccttt ccagatacgg
    15361 gaggcgaagt catttcttcc ttcgccctgg tggcaatctt tacgagcatt gtcacggcca
    15421 aagcaccgta tcagttgacc caggtgcaga ggctagagtg tttagtggtt aagccgatac
    15481 tagcgtgtaa acgagatttt taccatccac actttcgcta tgtaatcatg tatatagcgt
    15541 catgatcgtg ctatttcgct acagaataag ggaaaatggc ggttttactt tgatatacat
    15601 catcagggag gcatagcgta catcgtcctt tagaggtaga agaaaatagt ttgtatcctc
    15661 aatattggca ggttaattgc tgtttccccg caatttgcgc tactgtagcg cggataatct
    15721 gactccagga aactatatgc gcaatcgtac gctggctgat cttgatcgtg tcgttgctct
    15781 cggcggaggg catggactgg gacgcgttct ctcatcactt tcgtctttgg gttctcgttt
    15841 aacgggtatc gtcactacca cggataatgg tggctcgacg gggcgtattc gccgctcaga
    15901 agggggcatt gcctggggcg atatgcgcaa ctgcctcaac cagctgataa ctgaacccag
    15961 cgtcgcttct gcaatgtttg aatatcgttt cggcggtaat ggcgaacttt ccgggcacaa
    16021 ccttggaaac ctgatgttga aggcactgga tcaccttagc gtgcggcctc ttgaagcgat
    16081 taatctgatt cgcaatctgc tgaaagtgga cgcacatttg attccgatgt cggagcatcc
    16141 cgttgatctg atggcgattg acgatcaggg gcatgaagtt tacggcgagg tcaatatcga
    16201 ccagttaact acgccgattc aagagttatt gttaacgcct aatgtacccg caacgcgtga
    16261 ggcggttcac gctatcaatg aagcggatct catcattatt gggcctggca gtttttacac
    16321 aagcctgatg ccaattctgc tgctgaagga aatcgcgcag gcattacgcc gcacgccagc
    16381 gccgatggtc tatatcggca atctggggcg tgagttgagt ttacctgcgg ccaatttgaa
    16441 gctggaaagc aagctggcaa ttatggagca gtatgttggt aaaaaggtca ttgatgcggt
    16501 catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag cggattgtga ttcaggaggt
    16561 gctggaggcc agcgatattc cgtatcgtca tgaccgccag ttgttacata gcgcactgga
    16621 aaaggcgtta caggctttag gttaacggaa atccgaagga aaactccggc ttcctattga
    16681 agacaaagtg cgcgttgttt atgccggatg cggcttgaac gccttatccg gcctacaaac
    16741 cgcgctaatt caatgtattg cagaatcatg taggcctgat aagcgtagca catctggcaa
    16801 ttttgctttt gtcatctgtc ttcttcgtta tcctgtcacg aagccgcgat aaacaattca
    16861 cgcagctgat gcaactggtc acgaatttgt gccgcttctt cgaactccag attctgcgcg
    16921 tgttgcatca ttaacccttc cagctcatgg attttctgtt gcaacgcttt aggcgacata
    16981 tccatcggca cattatccgg ctcaacaatc ggtcgcgatt ttcctctgcc cttcgctttg
    17041 gttttagcaa tgttctgccc caacgccagg atatcgacca ctttcttgtt caagccttgc
    17101 ggcgtaatgc cgtgttcctc gttgtacttc tgctgtttct cgcggcgacg ttcggtttcg
    17161 ccaatcgctt tcgccattga cggggtgatc ttatcgccgt agagaatcgc tttaccgtta
    17221 acgttacgcg ccgcacgacc aatagtctga atcaatgaac gttcagaacg caggaagcct
    17281 tctttgtcag cgtcgaggat cgccaccagc gaaacctccg gcatatccag accttcgcgc
    17341 agtaagttga tccccaccag cacgtcgaac tcacccagac gcaagtcgcg gataatctcc
    17401 atacgttcga cggtgtcgat atccgagtga agataacgca cgcgctcgcc atgttcttcg
    17461 agatattcgg tgagatcttc cgccatccgc ttggtcagtg tggtgaccag tacgcgttcg
    17521 ttaattgccg ctcgctgacg aatctccgaa agaagatcat caacctgtgt cgccactggc
    17581 cgcacttcga taatcggatc aagcaatccg gttggacgca ccacctgatc caccacatcg
    17641 ccgccggatt tttccagctc ataattacct ggcgtcgccg aaacatagat ggtttgcggc
    17701 gctaatgctt cgaactcttc aaatttcagc ggacggttat ccagcgctga tggcaggcgg
    17761 aagccgtact ccaccagtgt ctctttacgc gcccggtcac cgcgatacat gccgccaatt
    17821 tgtggaatgg tgacgtgaga ttcatcgacg accagcagcc catcggcagg caggtaatca
    17881 aacagcgtcg gcggtggctc acccggtcca cgaccggaga ggaagcgcga gtagttttca
    17941 atccccgaac agtagcccag ctcgttcatc atctccagat caaactgggt acgctgggtc
    18001 agccgctgct cttccagcag tttgttgttt tccaatagca ctttgcgtct ggcggccagc
    18061 tcttctttga tctcctccat cgcctgtacg atgcgctcgc gcggtgtgac gtagtgcgtt
    18121 tttgggtaga tggtaaaacg tggaatggtg gaaacaatct gcccggttag cgggtcaaat
    18181 aacgacaatc gttccacttc ctcgtcaaac agttccacgc gaagtgcaat gtcatccgat
    18241 tctgccggga agatatcaat cacctcgcca cgaacacgga aagtaccacg ctggaatgct
    18301 tgatcattac gagcgtattg cagctccgcc agtcggcgca gaatcgcgcg ctgatcgata
    18361 atcataccga ccgtgagatg gagcatcatc ttgagatata aatcaggatc gcccagacca
    18421 taaatcgcgg aaacagacgc gaccacaaca acatcacgcc gctccagcat cgctttggtg
    18481 gcggacaaac gcatctgttc gatatgttcg tttaccgagg catctttctc aatgaaggtg
    18541 tcggaactcg gtacataggc ttctggctga tagtagtcgt agtaggagac gaaatattcc
    18601 accgcgtttt ccgggaagaa ctctttcatt tcgccataca actgggccgc cagcgttttg
    18661 ttgggcgcaa gtaccatggt agggcgctga aggtcagcaa tgacattggc aatggtgaag
    18721 gttttcccgg aaccagtcac accaagtaac gtctggtgcg ccaggccatc ttccagcccc
    18781 tcttcgagac gtcgaatcgc ctctggctga tcgccagaag gtttaaaagc ggaattcagt
    18841 ttgaacggtt tactcatgag tcgctacctg aaggagttgg gcgggcaggt atgtaatttt
    18901 actcgtcgta cttaattatg ccaacaaatt atactggata aaaaaacagt tcatcaccat
    18961 aatatttctg ttacagcgta aactccgctg tcaatcatga gtaaaattta ctctgtggcg
    19021 agataaaact cggaccttac cgggttatcc ccaaagcaac ggctttttta acatttgtca
    19081 agatgagtga tgtcagtttt atggcaagag atgcctgttc agtgactgcc attgcaatta
    19141 tctaaccagt taaaaaataa aagatatttc tttgagccgt ctttaacgcc aattcgcgtg
    19201 caagccgcgt attctctcgc ttgcctcgtg ttttctaact ctaatacaca tggttatcca
    19261 caggaatagt ggataactgt ctgcagcccc tatcccccgc cgcctgggca attcccacat
    19321 tcgccgcatg atgcggaagt aaaatttttt tcgtgattat tttagaattt aattggttaa
    19381 attgcagtca accgaagacg cgatctcgct cgcaatttaa ccaaatacag gatagctaca
    19441 acaaggcaag atctatgtac tttccggttg ccgcattttc tggattttct gcaagccagg
    19501 ggatctctcc cagcagcggc gcgggaatca tgcgggtgag cgtggtcata tattcagcgt
    19561 gacgttttcc aggaggcgtg acatcattcg ccacccaacc cgccagcgtc agtccggcgt
    19621 gttgtattgc ctgtgcagtc aacatcgcgt gattaataca gccgagtttc acgccaacca
    19681 ccagtattac cggcagttgt tcctgtgtta cccaatccgc aaaagtgaaa gtgtcagaaa
    19741 ggggcgtaaa ccagccgcca gcgccttcca ctaacaccca gtcagcctgt tgttcaagcg
    19801 cgcgtaatcc ggagctcatt accgatgatt ctatcggcct gccctcttgc gcgctgatga
    19861 tgtgcggtga ggtgggttct gcgaaggtgt aaggatttac cgttgcgtaa tccagctgca
    19921 ggctgctgtt gcgctgtaac gccagcgcgt cactattacg taaaccctcc ggggtctttt
    19981 cgctgccaga ggcgaccggt ttataacctg ccgtccgata gcctgctgcc tttgcggctt
    20041 gtaaaagtgc acaactggcg acagttttcc ccacttcggt atccgttccg gtgacaaaat
    20101 aacgtttact cacgaacaat cactcccaaa aaaagatgat acgtcagagg atatcgcccc
    20161 tgttgttgcg gccaggccag ttgcaatcgc tgcaactgtg aacgcgttaa tattcgctgg
    20221 tcgcgccctt catgaagatg cgtggcaccg atgcctttca gcgaacgcat ggcactgagc
    20281 gcatcatcaa accacagcgt gatgggctga atatgatgtc gataatgcac gccgttcagc
    20341 gactgttcga tttcatctgg cggtaaaaag cgattagcat gcgggcgctc gtccaccgcc
    20401 tgccacgcct gatgcagttc gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg
    20461 cctccggggc gcaccacccg atacagctcg cggagtgccg tggataaatt accgcaccac
    20521 tgcaccgcga gattgctcca tgcaagatcg aacgtcgcag tcgctaacgg cagggattcg
    20581 atatctcccg ccagataatg gtctgcagca tctttctggc gggcttgcgc aagcattggc
    20641 ggcgagagat ctaacgccgt cacctgcgcg tgacgctctc gccagtggcg gctcatccag
    20701 ccaggcccac accccgcgtc cagtacgtgg gtgtatttac gctgtgggag cattgccagt
    20761 aagacatcag cactctggcg ctgtaactct gcgtgttgct catagtgcgc ggctgcccga
    20821 ccaaatgccg ctgcaatggc ttgtttatta accgttgcca tgcagcacct ccagcagacg
    20881 gtcgatatcc tgcatttcat gcgcggcggt tagcgttaag cgcagtcgcg cagtaccaga
    20941 gggtacggtt ggcgggcgaa tcgccgtgac ccagcagcct tgctggcgca gtttttctgc
    21001 cagttgtaac gcacggccgt tatcaccgac gatcaatggc tgaatggcgc tgcatgaatc
    21061 agccagcgta aacggcaaat cctgtactcc ggcacgaaaa cgcgtaatga gtgccgccag
    21121 tttttcgcgc cgtgcatcac cctcatcact gcgaatgacc gccagcgacg cacgtaatgc
    21181 ctgcgcctga gcgggcggca tactggtgct gtagataaga tggcgggcga attgcagcag
    21241 ataatccgcc accgtattgg agcaaagcac cgcagccccg ctgacgccaa atcctttgcc
    21301 aaaagtcact accagcaatt ctggttttac cttttgcaac cagcaggtgc cgcgcccctg
    21361 ctccccgata acgcccgtgc cgtgggcatc atcgaccatc aaccagccat tgtgctgttg
    21421 cgttacctgc tggatttccg ccagtggcgc actatcgccg tccatgctga acacgccttc
    21481 tgtcaccacc agttgctgcc ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac
    21541 atcgttatga acaaaacggc gaagctgcga cgggcttaaa ctggcagcct ccagcaatga
    21601 ggcatggctt agccggtcag cgacaatgcg gtcctctttt gccatcatcg cggcaatgac
    21661 cgcctgatta gcggcaaaac cagagataaa cagcagtgcc cgcgaatagc ccagccactc
    21721 ggccagttct tcttccagcg cctgatgtgc cacgctataa ccgctgacgt gaccggagcc
    21781 gccgctaccg acgccaaatt gctccgcacc ctgcttccag gcacggataa tttgcggatg
    21841 atggcttaaa cctaaataat cgttactgga aaagttcaga tactggcgat cgtccgccac
    21901 cagccagcgt ccggctcctt gcgccactgg ataacggcga cgcagggcat cggcagcacg
    21961 ccgcgcatcg agcgccgcgt tgattttctc ctgccagctc atagtgctgc cgcgttgtaa
    22021 tattcgtcag tgtccggggt catcagtgcc tgttccagac gctgctgttg ttcgttatcg
    22081 cccgccagca cggcagtttg ctgcggattt agccccagtt tgcggaacag ttgcaggtct
    22141 ttatcttctt ccggattcgg cgtggttagc agtttgcaac cgtagaaaat cgagtttgcg
    22201 ccagccataa agcacattgc ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg
    22261 cgtacgtaag aggtcggcat catgatccgc gctacggcaa tggtgcgaat aaaatcaaag
    22321 gcatcgacat catcgttatc cgccagcggc gtgcctttca ccttcaccag catgttgatt
    22381 ggtacgcttt ccggcggcgt cggcaggttt gccagttgca gcaataatcc ggcgcgatct
    22441 tttaccgttt cgcctaagcc cacaatgcca cctgagcaga ctttgatccc ggcatcgcgc
    22501 actttttcca gcgtatcgag gcgttcctga taagtgcggg tggtgatgat attgccgtaa
    22561 aactccggcg aggtatcgag gttatggttg tagtaatcca gcccggcgtt cgcaaggcgc
    22621 tgcgcctgag attcactcaa cgtacccagc gtcatacacg cctcgaggcc catcgctttt
    22681 accccttgca ccatctgctc caggtacggc atatcgcgtt cgtggggatt cttccacgcc
    22741 gcgcccatac agaagcgcgt cgatcctgcc gctttcgctt tgcgcgccga ctccagcacc
    22801 tgttcaactt ccatcaaccg ctcggcttcc agcccagttt tgtagcgcga gctttgcggg
    22861 cagtatttgc aatcttccgg acaagctccg gtcttaatcg acagcaacgt gctgacctgc
    22921 acctgacgag gatcgaaatg ctggcgatgc acctgctgcg cttcaaacag cagatccagc
    22981 aacggttttt caaataattc tgtgacttgc gacaatgtcc agcgtgggcg gtgagccatg
    23041 gggcttctcc aaaacgtgtt ttttgttgtt aattcggtgt agacttgtaa acctaaatct
    23101 tttcaatttg gtttacaagt cgattatgac aacggacgat cttgcctttg accaacgcca
    23161 tatctggcac ccatacacat ccatgacctc ccctctgccg gtttatccgg tcgcgagcgc
    23221 cgaaggttgc gagctaattt tgtctgacgg cagacgcctg gttgacggta tgtcgtcctg
    23281 gtgggcggcg atccacggtt acaatcaccc gcagcttaat gcggcgatga agtcgcaaat
    23341 tgatgccatg tcgcatgtga tgtttggcgg tatcacccat gcgccagcca ttgagctgtg
    23401 ccgcaaactg gtggcgatga cgccgcaacc gctggtatgc gtttttctcg cggactccgg
    23461 ctccgtagcg gtggaagtgg cgatgaaaat ggcgttgcag tactggcaag ccaaaggcga
    23521 agcgcgccag cgttttctga ccttccgcaa tggttatcat ggcgatacct ttggcgcgat
    23581 gtcggtgtgc gatccggaca actcaatgca cagcctgtgg aaaggctatc tgccagaaaa
    23641 cttgtttgct cccgccccgc aaagccgcat ggatggtgaa tgggatgagc gcgatatggt
    23701 gggctttgcc cgcctgatgg cggcgcatcg tcatgaaatc gcggcggtga tcattgaacc
    23761 gattgtccag ggcgcaggcg ggatgcgcat gtaccatccg gaatggttaa aacgaatccg
    23821 caaaatgtgc gatcgcgaag gtatcttgct gattgccgac gagatcgcca ccggatttgg
    23881 tcgtaccggc aaactgtttg cctgtgaaca tgcagaaatc gcgccggaca ttttgtgcct
    23941 cggtaaagcc ttaaccggcg gcacaatgac cctttccgcc acactcacca cgcgcgaggt
    24001 tgcggaaacc atcagtaacg gcgaagccgg ctgctttatg cacgggccaa cttttatggg
    24061 caatccgctg gcctgcgcag cagcaaacgc cagcctggcg attctcgaat ctggcgactg
    24121 gcagcagcag gtggcggcta ttgaagtaca gctgcgcgag caactggcac cagcccgtga
    24181 tgccgaaatg gttgccgatg tgcgcgtact gggggcaatc ggtgtggtcg aaaccactcg
    24241 tccggtgaat atggcggcgc tgcaaaaatt ctttgtcgaa cagggtgtct ggatccggcc
    24301 ttttggcaaa ctgatttacc tgatgccgcc ctatattatt ctcccgcaac agttgcagcg
    24361 tctgaccgca gcggttaacc gcgcggtaca ggatgaaaca tttttttgcc aataacggga
    24421 agtcagcgtg agggtttctg gctacacttt ctgcaaacta gaaaggaggg ttcatgaaac
    24481 tcatcagtaa cgatctgcgc gatggcgata agttgccgca tcgtcatgtc tttaacggca
    24541 tgggttacga tggcgataat atttcaccgc atctggcgtg ggatgatgtt cctgcgggaa
    24601 cgaaaagttt tgttgtcacc tgctacgacc cggatgcgcc aaccggctcc ggctggtggc
    24661 actgggtagt tgttaattta cccgctgata cccgcgtatt accgcaaggg tttggctctg
    24721 atctggtagc aatgccagac ggcgttttgc agacgcgtac cgattttggt aaaaccggat
    24781 acgatggcgc agcgccgccg aaaggcgaaa cccatcgcta catttttacc gttcacgcgc
    24841 tggatgtaga gcgtattgat gtcgatgaag gagccagcgg cgcgatggtc gggtttaacg
    24901 ttcatttcca ctctctggca agcgcctcaa ttactgcgat gtttagttaa tcactctgcc
    24961 agatggcgca atgccatctg gtatcactta aaggtattaa aaacaacttt ttgtcttttt
    25021 accttcccgt ttcgctcaag ttagtataaa aaagcaggct tcaacggatt catttttcta
    25081 tttcatagcc cggagcaacc tgtgaacaca ttttcagttt cccgtctggc gctggcattg
    25141 gcttttggcg tgacgctgac cgcctgtagc tcaacaccac ccgatcaacg tccttctgat
    25201 caaactgcgc ctggtacctc ttctcgcccg attctgtcgg caaaagaagc gcagaatttc
    25261 gatgctcaac actattttgc atccctgaca ccaggtgctg cagcgtggaa tccttccccg
    25321 attaccctgc ctacacaacc tgactttgtt gtcggcccgg cgggtactca aggtgtaacg
    25381 cataccacga ttcaggcggc ggtagatgcg gcaattatca agcgtaccaa caagcgccag
    25441 tatattgccg tgatgcctgg tgagtatcag ggaacggtgt atgtccctgc cgctccgggt
    25501 ggaattactc tgtacggtac aggtgaaaaa ccgattgatg tgaagattgg gctttccctt
    25561 gatgggggta tgagccctgc cgactggcgt cacgacgtca acccgcgcgg caaatatatg
    25621 ccaggtaaac cagcgtggta tatgtacgat agctgccaga gcaaacgcag cgacagtatc
    25681 ggtgttctct gctctgcggt cttctggtca caaaacaatg gcctgcaact gcaaaacctg
    25741 accatcgaaa acacgctggg cgatagcgta gatgcgggta accatccggc ggtggcactg
    25801 cgtactgatg gtgaccaggt acagattaat aacgttaaca ttctcggtcg tcagaacacc
    25861 ttctttgtca ccaacagcgg tgtgcagaac cgtctggaaa cgaatcgtca gccgcgtacg
    25921 ctggtgacca acagctacat tgaaggggat gtggatatcg tttctggtcg cggcgcagtg
    25981 gtgttcgata acaccgaatt ccgcgtggtg aactcacgta ctcagcaaga agcgtatgtg
    26041 tttgcaccgg ctacgctgtc caacatctat tacggtttcc tcgccgtaaa cagccgtttc
    26101 aatgcttccg gtgatggcgt ggcgcaactg ggccgctcgc tggatgttga tgccaatacc
    26161 aacggtcagg tggtgatccg tgatagcgcc atcaacgaag gttttaacac ggttaaaccg
    26221 tggggcgatg cggtgatctc taatcgtccg tttgcgggta ataccggcag cgttgacgat
    26281 agcgacgaaa tacagcgcaa tctgaatgac actaactaca accgcatgtg ggaatacaat
    26341 aaccgcggcg tgggtagcaa agtggttgca gaggcgaaga agtaagagct attaactatt
    26401 tgccggatgc ggcgtaaacg ccttatccgg cctacggttc gatgcgattt gtaggtcgga
    26461 taagatgcgc cagcatcgca tccgacaata agtgccgaat gcgacctaca ttcacatggc
    26521 gctttttaca tctgacggtt tttattgaag ttaatcaaac tacccgcctt gataatctcg
    26581 cgctcttcag cagtcaaact ttccatatag agcgtaatct ccgttaccgg cgcatcttca
    26641 tggatcacat aacctttaaa cgtcgtaccc ggattatcca gcgccgcttt aatgccaggg
    26701 atgtaaatgt aatcccccac ttcaaaggtt ggcacttccg ccatctgcag cggtaacatc
    26761 ccccagttga tgacgttaga acggtagcgt ttagtcgcgt actcctcggc aatattcgcc
    26821 agaccgccaa tcacacgctg gcagctcgcc gcctgttcac gcgcagaacc atcgcctggt
    26881 ttcaccgcat agaccatgct gccaatttca gtttgcagcg gatcaatatg ctcctgacca
    26941 gcaatctgct taatgcgcgc aaacacctct gtcagctcgc tgacattccc cgccagacgc
    27001 tgattttcca gctcagcagt cgctttactt ctgccaacat aaccgggatc gcggcgtgac
    27061 agggtaaact ccgccagacc aatcggattt gaacgataag aagaggtttc accggaagga
    27121 atcagttcgt cggtggtggt cacttcgtcg aggatcttcg agcacacttt gaggacgata
    27181 ttgtcagtca gcgcacccaa ttccggccag tctttaatgt tcggtccgta aatcagcggt
    27241 tgctgggtcg cccctttcac aaagccttga tagacgcgat ttttatacgg cgttacatcg
    27301 aaggcgtact ccggcacgtt gtcccagcaa tcgagttcgc tggcagaggt taaatagcca
    27361 ccgtttgccg cagtcgcagc gatagaacga gcgtccatca acgccaccgc tgacatctgc
    27421 ccattagctg gcttagagcc ttcgcggttc gggaagttac gcgtggtgtg gcgaatactc
    27481 aaaccgttgt tgattggcgt atcgcccgcg ccaaagcatg ggccacagaa cgcggttctg
    27541 ataattgcgc ctgcgccaat caaatctgct accacacctt ttttggctaa atccataaac
    27601 accggctgtg atgacgggta aaccgccagc gagaaggtgt cattgccaca ggattgaccg
    27661 cgcagtgcat tcgccgccgc gatgacgttt tcgtagttac cgccagaaca gcccgcgata
    27721 atcccctgct gcactttcag gcgaccattt tccactttgt ccagcagcga gagtttggct
    27781 ttaccgtgcg ccacgcgttc ggactcaatt tcaatctcac gcagaatgtc ggtcaggttc
    27841 tggttcagtg tgtcgatttc atacacgttg ctcgggtgga acggcagcgc aatcattggt
    27901 ttgatggcgc ttaaatcaac gctgatgcag ccatcgtagt acgccatcgg ttgagggtta
    27961 agctggcagt aatcctggcc gcgaccgtgc agcgccagcc agttatggac ttcttcatcg
    28021 gtttgccaga cagaacttaa acaggtcgtt tcagtggtca taacgtcaac gctgttacgg
    28081 aaatcggtag agagcgcagc aacaccggga cctacgaatt ccatcacttt gtttttgacg
    28141 tagccgtttt tgaacacggc accgatgata gccagcgcca catcctgcgg ccccacatac
    28201 ggcgctggtt ttccggtcag atgcaccgca acaactcccg gatagtcgat atcccaggtg
    28261 tcattaagca attgttttac caactcgccg ccgccctcac cgactgccat tgtccctaat
    28321 gcaccgtaac gggtgtgact gtctgacccg aggatcattt tgccgccacc tgccatcatc
    28381 tcacgcatat attgatggat gaccgcaata tgcggaggca caaaaatacc gccataacgc
    28441 tgagccgccg ataaaccaaa aacatggtca tcaccgttaa tggtgccgcc gacggcgcaa
    28501 agtgagttat ggcagttggt cagcacatac ggcagcggga aacgttccat accggacgct
    28561 ttagcggtct gtacaatacc aacaaaggta atatcgtgag aggctaatga atcaaactta
    28621 attttaagtt tatccatatt tccggacgta ttatgagaag agagaataga ccaggcaata
    28681 gtgccttttt tggcttcttc ttttttaatt tggccggtga aatgttcttc ggcaattatt
    28741 tcgttattgc tggcgagaaa cacgcctttt tcagataact tgatcattaa atgctccaga
    28801 tatctgcgta ccgtgaagtg cgcagaaaga taataaatgg atatactcta aataattcga
    28861 gttgcaggaa ggcgacaagc gagtgaatcc ccaggagctt acataagtaa gtgactgggg
    28921 tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtatatt tacagcatgt
    28981 tccagccgat cagcatattc caccaccaca cgccgagggt gatatgcacc aggaaggtta
    29041 atatcgtcag taccgcaccg accaaccacc aggatttaat atcgttgtaa cccacgccaa
    29101 agatgaccgg acctgccgcg ccgccatagt gagtaaccat gccgccatag gagttggaga
    29161 acaacagtgc cagcgcggtt aacattaacg gtgcgccgga gacgttcgcc agcatggcaa
    29221 atacaggtaa catagcaacg atataggcac taccggaagc gaagaaataa cgcacaatga
    29281 tgctgaggaa aataataacg aagaaagcaa cgttaccgtg accatcaaat gccaggttat
    29341 ttttaaagac ttcagctaac cattcgaaga atttaacttt cgataataag gagcttaagc
    29401 cgataatacc gccgtaccaa attaaagtat tccagccgcc tttattttta accacgtctt
    29461 cccaggtaac aatacccagc agcagcatgg ttgccataac aacgattgcc acggtggatt
    29521 catcaacccc cagagactta ctgaaaatcc agcccagcag cgccagcaca aagacaccga
    29581 gcagcatttt ttcgcggatt ttcatcggtc ccagttcggc aaggcccgct ttagcgatgg
    29641 ttttgttatc caccttctta atttctggcg gatacatggt gtaaatcacc agcggggtga
    29701 ccagcagcat aatgatgccc ggcaatccgg ctgccagcgc ccatccaccc cagctaattt
    29761 gcaggtgcag aatgtcgttg atcattttca gcgccagaat gttccccgcc attgcggtaa
    29821 agaacatata gctggtggtt ttggtgacca tgtaaatgga catcatcagg taatgtccga
    29881 cacgacgcgg acttttttcc ggttcggacc ccaaagccac cgccacgctg ttgatgatcg
    29941 gtaacacaat gccgcccgca cgcgcggtgt tagacggtgt tgccggagcc agtaccagat
    30001 cgaggaatac cgtaacgtaa cccagaccca gcgtggtgtt accgatttta ccaatcagca
    30061 gataggcaat acgtttacct aaaccggtgg tcacaaatgc ggcgcttaag gtaaacgccg
    30121 agaacaccag ccaggtagta cctgaagagt aaccgcttaa tacggcggtg gtcttaaacg
    30181 caccgtcgga taagttaccg accaccacca tggaggcagc aacggcaatt aacagtacga
    30241 caggttccgg gaaaggcttg ataaccagcc ccacaatggc cgccaggtaa ataccaaaaa
    30301 gcacccacgc cagttcgctt aatcctgccg gagctggcat aaagccaata atacatggga
    30361 tcgctaatat cagaattagc ttccataacg atttcttatt catttaaaat cctgttcttt
    30421 ctgtatgact catacagaag tcctttattg attgtcctta tcctgacaac gaatcttttc
    30481 agggaagata aacttcaccg gaaaatattt ttctggtcgt ccgaataaca gatgcgcgta
    30541 acgtcgtggc atcctgacct tcattactta aatgaacgtc gagcgcacca ctggggtgtt
    30601 caatattgat attgccatat cctacagaag ggatgatttg tcgggtgacg gtgccttcca
    30661 atgcacaact actggaaata gcaatagcac cggttatcgc caacgcgcga tggcaagaat
    30721 gcggcataaa ataacgcaca ttaattgccc cgcctttctg cgctggagaa ataagcacag
    30781 gtttagggat aaccatatta ctgacatcac ctaagcccat tgctttaccc gcttgtagac
    30841 ggatagattc aatgcgggct aataatgctt tgtcggcatc cagttccgcc ggtaattcat
    30901 agcctgtttt tcccagatat tcagccggaa taatgacgac tggcatcgcc atatcgatac
    30961 aggtcaccgg gacatcgtca aaataatcaa tctgattatc agtcgggaaa acttttccgg
    31021 ttttcgttcc agcggcattc aggaaagtga gcgcaaccgg tgcggcagta cccggtacgc
    31081 cgtcaattct ggcgctaccc tcgtactcga caacaccatt tggcgtttgc acatcagctt
    31141 cgatgaacgt acccgtattg acgttgcgga tacgtacgcg ggtaactggc gaagtcgctg
    31201 caatcaaacc attttcaatg gcgaatgccc caacgccaga cagcatattg ccgcagttag
    31261 gcgtggtatc gacacgttgc tcatggacga taacttgagc aaacagataa tcaacatcag
    31321 cacgcggatc gctggaacgg cttataatgg cgactttact ggtcagtgga ttaccgccgc
    31381 caataccgtc aatttccaga tcgttaccgg aacccataat tgccatcaat attttatcgc
    31441 gctgcgtttg atcttcgggt aaatgttccg ctaacaggaa cgcgcccctc gaggttccac
    31501 ctcgcatcat cacgcagggt atttttttca taataatgcc ctcaatttaa aaacataact
    31561 tattgcgata tggttacatt aagggcaaag catctctaat gactttagct caacgattaa
    31621 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa gggccagcca
    31681 cccaaaatca acgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
    31741 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaacat
    31801 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
    31861 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
    31921 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcggg atatgaatgc
    31981 ttatcatcgt ggtatggtga cgttagcgtg tattccgacc gcagtatttt attttttacc
    32041 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
    32101 aggcacgaat aattgcatgg aatcggtgct atgtaacgaa tcagactttg gcataaacat
    32161 gaacaacgtc acaaattcat ccattgattt tagcccactg gtcaacgagc cgttcgtgct
    32221 ggcctgtcgg cgtgaccatc cattggccaa aaagcagctt gtagaatggc tggagttagt
    32281 gggttacaaa atgattggcg tacgctcctc cagcggcaac cgactgttga tagaacaaca
    32341 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
    32401 attaggactg gtggaggcag gactggggat ttcagcgctt cctggtctgg caatgcccca
    32461 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
    32521 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
    32581 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
    32641 gagacatgcg ttacaggaga taggttaata caccggcgca ggagagactg cgccgggtaa
    32701 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcgtagcgg
    32761 cctttttcat gcagtagccc ctgctcacca acaatttcgt ataccgagat gtggtgagat
    32821 ttttgcccgg cggcaatcag atacttgcca ctgtgatcaa cattgaagcc gcgcggctgg
    32881 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
    32941 acggtaatca gactggcggt acggtcgcag gcgtataaat gacgaccatc cggggtgata
    33001 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
    33061 acacattcga tattgccgtg tggatctttc agttcccaga catccactga gctgtttaac
    33121 tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
    33181 ccttcaacgg tagtcacttc cgcaggatcc tgcaccacca gatgaccatc atcgctgacc
    33241 gtaaacaggc aaatgcgatc ctgctttaat gccggaaccc acagcgtacg gttgtccggt
    33301 gagatattgg cggaatggca accgtccagc ccctcgacca catcgacgac gcccactggc
    33361 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
    33421 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcgc agactctgcg
    33481 gcaaaggtca gtgcgccatc gtccggggcg atacgatacg ccaggacgcg aaactcaggg
    33541 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
    33601 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
    33661 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaatgc tcctttgcat
    33721 ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc tgaattaaaa
    33781 cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagaagaa aattcttaac
    33841 cttaatctgg aatacgccat gaccacacgc gtgattgctc tcgacttaga cggcacctta
    33901 ttgaccccga aaaagacctt gcttccatca tcgatagaag ccctggcccg cgctcgcgaa
    33961 gcaggctatc aattaatcat cgtcacaggt cgccatcacg tcgctattca tcctttttat
    34021 caggcgctgg cgctggatac acctgctatt tgctgtaatg gcacctattt gtatgattat
    34081 catgcaaaaa ccgtgctgga agcggaccca atgcccgtta ataaagccct gcaactcatt
    34141 gagatgctga atgaacacca cattcacggt ctgatgtatg tcgatgatgc aatggtctat
    34201 gagcacccga ccgggcatgt cattcgcacg tctaactggg cgcaaaccct gccgccggaa
    34261 cagcgtccga ctttcacaca agtcgcttct ctggctgaaa cggcgcaaca agttaacgcc
    34321 gtatggaagt tcgccctgac gcacgatgac ctgccgcaat tgcagcattt tggtaagcat
    34381 gtcgaacatg aactgggact ggagtgtgaa tggtcctggc acgatcaggt tgatattgca
    34441 cgcggcggca acagcaaagg taaacgtttg acgaaatggg ttgaggcgca aggctggtcg
    34501 atggaaaacg tcgtggcgtt cggcgataac tttaatgata tcagtatgct ggaagccgct
    34561 ggtacaggcg tggcgatggg caacgccgat gacgcggtaa aagcgcgcgc caacattgtg
    34621 attggtgata acaccaccga cagcattgcc cagttcattt atagccacct gatttaatca
    34681 ggcggttatc gacacacttt taatttgcgc gtacagccac aggccaggtt tgatcgccag
    34741 ttcatccctg gcccacgggc tgatacgcgc ccacagcgtt ttaccgccga cttccagttc
    34801 cacttccacc tggccgttgt cgtcataact attaacaact tttgcccgca atacgttacg
    34861 aatgctggtt tgctgcggcg gttgtaaaac cagagaaaca tcggaagcct gaatgcggat
    34921 acgtagcgca gcttgcagcg gttcgtccag cttattgacc cacaaatgct gatcgcccag
    34981 cgccagcgcg gtcatcgcgt aatgcggatg atgctcaagc accgtcactt tcagaatgct
    35041 actttgttgc tctttcggca gccacggatt catcacgcta ctaccccaga cttcttccag
    35101 cgcgccaaag gctttcacct gaccgttttc cagtaccatc actctgtctg ccagatggag
    35161 gatctcatcc agcgaatggc tgacatacaa catcgggatg ttgatttccc gcgtcagacg
    35221 ttgcagataa ggcaacagtt cgcgtttacg cggaatatcc agtgacgcca gcggttcatc
    35281 cagcaacagc aattctggtg ccgtcagcaa agcccgccca atcgctacgc gctgtttttc
    35341 gcctccggac aggctgcctg gtaaacggtc aagcaacggt tcaatgccta aaagcgccac
    35401 cagcttatcg aactgatcga ccatgctttt cgccatgccg tagcgcagat tgccacgcac
    35461 tttgtaatgt ggaaacagcc gcgcatcctg aaaaacatag ccaacgcgac gcttttctgg
    35521 cgtcaggcag ataccttttt cggcatcatt tagtacccgc ccattgagga caatcctccc
    35581 tttttgcggg cgcgtcagtc cactgatggc gttaatcagc gaagtttttc cggcaccgga
    35641 gacgccaaag atagcagtga tgccattggc gggcagcgtt tcattaatag tcaggcaatg
    35701 gttgcccaac gtctgggaaa aattcagttc cagcatgatt agcgccccgc ccgttcacgg
    35761 ctgattctgg ccagccattc tgaaatcaac agggagatca tcgccagcgc aatagaaata
    35821 atgcacagtc tcgctgcgcc actttccccg ccgggggtct gaatcagggt atacatggca
    35881 gaaggaatgg ttcgcgtttc accaggaatg ttcgacacaa aggtgatggt tgcgccaaat
    35941 tcaccgagag aacgagcaaa agccagtacc gtaccaacaa taatgcccgg taaggtcagc
    36001 ggtaacgtga tagtaaagaa aacgcgccag cgcccggccc ccagtgttct tgcggcctgt
    36061 tccagtttga catcaacccc ttccagcgcc agacgaattg cccgcaccat cagcggaaac
    36121 gacatgacgg cggcagcgag aaccgcgccg cgccagctaa aggcgaaggt aatgccaaac
    36181 cagtcataca aacgttcacc gataaatccg cgccgtccca tcgaaactaa taataagtaa
    36241 ccgacgacca cgggcggtaa caccagcggt agatgcagta cgctgtcgag cagagctttg
    36301 cccggaaacg tgcaacgcac cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac
    36361 agcacagcca gggaagaaac tttcaggctt aataaaactg cctgccattc tggatcggtc
    36421 agtatcatta cttggttgta aatccgtaac gtttaaagat ttccgctgcc tgcggtccct
    36481 tcagataatc ataaaatgct ttcactgtcg cattgttatg cccttccaca acagcaaacg
    36541 gatattccac ttttttatgt gaatcttccg ggaaggtggc aaccactttt acccctttgc
    36601 tggcaactgc gtcagaaccg taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg
    36661 ccagcgcgcc acgaacatct tccgccggag ccagtttcgg ggagagcgta tcccatgcgc
    36721 ccagtttttg cagtgcttct tttgcataaa tgccagcggg aacatgttcc ggatcgccaa
    36781 ccgccaggcg accgccattc agcagtgaag tccagttggt tttgctgtcg atggtaaaat
    36841 ctttctgctc gctggctttc ggtgctacaa cgaccaggct attgccgagc agtgtctgac
    36901 gcgtagctgt atcgatcgct tttttatcaa ccgcataatc catccatttc tgatcggcag
    36961 aaataaacag atccgcaggg gcacccgctt caatctgacg ggcgagagta gatgacgaag
    37021 cgaaagaaga aaccacatcc acgcctttct cttttttata ctgcgtagca atgtcctgca
    37081 ttgcgttagt cagtgatgcg gcggcgaaca ccgtgatttt cccttcatct gccagtgcat
    37141 tgccagcaac agcgaaagag agtgccgccc cagcaaacaa gttcaaccat ttacgagcca
    37201 ttggtaaccc cttaatgtaa cgttatgtag gcgacaatat aacgactaac tcggaatttt
    37261 cccagcagtt attgctaacc tttgtgaggt agataagaaa aaatatcggc aggaaaagca
    37321 ggaagttgag aaaaagaaaa tgcccgacta agcgggcatt cagggaatca atgattttgt
    37381 ccaggctggt cttttttacc aacaccagaa aagatgttga atacttcacc aagaccgtaa
    37441 atcagaccca ggatgatggc catcacgaca ggtaccatga ttacggcgaa taccagactt
    37501 tttaataact ctaacatggt caactccaga tatagtcatg agactattct aaccgctaag
    37561 cacagaaaag cactcccctt ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca
    37621 ataagacttt tgccaggaca ttgttatgca ggccgaaatc cttctcaccc ttaagctcca
    37681 acaaaaatta ttcgccgacc cgcgccgcat ttcgctacta aaacacattg cgctttccgg
    37741 ttccattagc cagggagcga aagatgccgg tatcagctat aaaagcgcct gggatgccat
    37801 taacgagatg aatcagttaa gtgagcatat tctggtcgag cgcgcaacag gcggtaaagg
    37861 tggcggcggc gcagtactga cccgctatgg tcagcgactg attcagctct atgacttact
    37921 ggcgcaaatc cagcaaaaag cctttgatgt gttaagtgac gatgacgccc tgccgctgaa
    37981 cagcctgctg gccgcgatct cacgtttttc actgcaaacc agcgcccgta accagtggtt
    38041 cggtaccatc accgcccgcg atcatgatga cgttcaacag catgttgatg tcttactggc
    38101 tgatggaaaa acacgcctga aagtcgcaat taccgcacaa agcggcgcgc gtctggggct
    38161 ggatgaaggc aaagaagtgt tgatattgct aaaagcgccg tgggtaggca ttactcagga
    38221 cgaagcggtc gcgcaaaacg ctgacaacca attaccgggt attattagtc atattgagcg
    38281 cggcgcagag cagtgcgaag tattaatggc gctaccggac gggcaaacac tgtgcgccac
    38341 agtgccggta aatgaagcga cttctcttca gcaaggacag aatgtcacgg cctactttaa
    38401 tgccgacagc gtgattatcg ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca
    38461 cgtatccctg tcagtaatcg ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt
    38521 tgcaaggcac gtttcgtctt agcgacacaa aaacgctgca attgcctcag ctaacgttaa
    38581 acgcgggtga tagttgggcg tttgtcggtt cgaatggaag cgggaaatcg gccctggccc
    38641 gcgcgctggc gggggaactt ccgcttttga aaggtgaacg gcaaagccag ttttcccaca
    38701 tcactcatct ctccttcgag caattgcaaa agctcgtcag tgacgaatgg cagcgcaata
    38761 acaccgatat gctcggccct ggcgaagatg acaccgggcg taccacggct gagatcattc
    38821 aggatgaagt taaggatgca ccgcgctgca tgcaactggc gcagcagttc ggtattaccg
    38881 ccctcctcga ccgacgcttt aaataccttt ccactggtga aacgcgtaaa accctgctct
    38941 gtcaggtact gatgtcggag cctgacttat tgattcttga tgaaccgttc gatggtctgg
    39001 atgttgcctc acgtcagcag ttggctgagc tactcgcctc gttacatcag tccggtatta
    39061 ctctggtact ggtgctcaat cgcttcgatg agatcccgga atttgtccag tttgctggcg
    39121 tgctggcgga ttgtacgtta gcggaaacgg gcgctaaaga ggaactgctc cagcaagcac
    39181 tcgtcgcgca actggcgcat agcgaacagc ttgaaggtgt gcaactgccg gagccggatg
    39241 aaccttcagc acgtcacgcc ttacccacca acgaaccgcg cattgtgctg aataatggcg
    39301 tggtttctta taatgatcgc cccattctta ataaccttag ctggcaggtg aatcctggcg
    39361 agcactggca aattgtcggg ccaaatggtg cgggaaaatc gacgttatta agcctgatta
    39421 ctggcgatca tccgcaaggt tacagcaacg atttgacgct tttcggacga cgtcgtggca
    39481 gcggcgaaac catctgggat atcaaaaagc atatcggtta cgtcagcagt agtttgcatc
    39541 tggattaccg ggtcagtact actgtgcgta atgtgattct ttctggctat ttcgattcga
    39601 ttggcatata tcaggccgtt tcggatcgcc agcaaaaact ggtgcagcag tggctggata
    39661 ttctcggcat tgataaacgc acggctgacg ctccgttcca tagtctttcc tggggacagc
    39721 agcgtctggc gctgatcgtc cgcgcactgg tgaaacatcc gacgttgctt attctcgatg
    39781 aaccactaca ggggcttgat ccgctgaatc gccagcttat ccgccgtttt gttgatgtgc
    39841 tgattagcga aggtgaaacg caattgttgt ttgtttcgca ccacgctgaa gatgcgcctg
    39901 cctgtattac ccatcgtctt gagttcgtgc cggacggtgg actctatcgc tatgtgctga
    39961 caaaaatata atgagtcggt agtgctgacc ttgccggagg cggccttagc gccctctccg
    40021 gccaacggtt cgacgcatgc aggcatgaaa ccgcgtcttt ttttcagata aaaagcgtaa
    40081 tcattcataa accctctgtt ttataatcac ttaatcgcac ataaaaaatg gctaaattct
    40141 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
    40201 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtc accggtggta
    40261 gcggttacat tggaagtcat acctgtgtgc aattactgca aaacggtcat gatgtcatca
    40321 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
    40381 gcaaacatcc aacgtttgtt gaaggcgata tccgtaacga agcgttgatg accgagatcc
    40441 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gttggcgaat
    40501 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggtactctg cgcctgatta
    40561 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
    40621 gcgatcagcc caaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
    40681 acggcaaaag caagctgatg gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg
    40741 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
    40801 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caggttgctg
    40861 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gacggtactg
    40921 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
    40981 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
    41041 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
    41101 caccgcgtcg cgagggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
    41161 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
    41221 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
    41281 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
    41341 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
    41401 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
    41461 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
    41521 cagatgcgcc agaaagcaac gaccctttga tgcgttgcca gagcgcgcgc ggtaccagcc
    41581 gggtgatctg cttttcaccg gatcacagta aaacgctgcc agagctcagt gttgcggcat
    41641 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga actggggaaa acgtacccat
    41701 gggtgcaggt ctttgaaaat aaaggcgcgg cgatgggctg ctctaacccg catccgcacg
    41761 gacaggtttg ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa
    41821 aagaatattt tgccgagcag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
    41881 cagacggtag ccgtaccgtt gttgaaaccg aacactggtt agccgtagtg ccttactggg
    41941 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttacgg atcaccgatt
    42001 tgaccgacgc ccagcgcagc gatctggcgc tggcgttgaa aaagctgacc agtcgttatg
    42061 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgcg ccatttaatg
    42121 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ttgcgctccg
    42181 ccaccgtacg taaatttatg gtcggttatg aaatgctggc agaaacccag cgtgatctga
    42241 cggcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
    42301 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
    42361 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
    42421 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
    42481 caccacgcga tgaccgtaaa gttcgcgtaa tggcagctga ttatgaaaat cagctcgacg
    42541 agttttccct cgatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
    42601 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
    42661 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
    42721 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
    42781 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
    42841 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgtcgctca ctggggacca
    42901 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
    42961 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
    43021 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    43081 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    43141 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt
    43201 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    43261 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    43321 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    43381 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    43441 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    43501 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    43561 tttactttca gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    43621 tgccagcccg gaatgctatc aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta
    43681 tgctaaccgc atcgccaata gccgttatac ctttgacggt gaaaccgtga cgctgttacc
    43741 aagtcagggc gtcaaccaat tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    43801 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    43861 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    43921 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    43981 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    44041 ggacgaatat ctgccggttg atgaaggcgg cattccccgc gacggcctga aatctgtcgc
    44101 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    44161 cgatcagcgc aaagtgaaag gttacgatca cgcgttcttg ttacaggcca aaggcaatgg
    44221 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    44281 caccgctccg gctctgcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    44341 aaccgaacct tacgccgact ggcaagggct ggctctggaa agcgagtttc tgccggacag
    44401 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    44461 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    44521 atcttcacat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    44581 caaccttaca ctgcgacact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    44641 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg
    44701 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    44761 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    44821 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacctct gtgctgaaac
    44881 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    44941 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    45001 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgccgtga
    45061 ctccgccgga actgactaaa gatgatgagc ggtatccggg tcacgatccc cgttacgcga
    45121 aactgagcga gaaagaactg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    45181 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    45241 ctgcacacgg taactcgtta cgtgcgctgg tgaaatatct cgataacatg agcgaagaag
    45301 agattcttga gctgaatatc ccgaccggcg tgccgctggt ttatgagttt gacgagaact
    45361 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    45421 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaatga gaaagccgac
    45481 tgcaagtgag tcggcttttt tgtttgtcgt tgctaacaat ggagcacatt gcctgatgcg
    45541 acgctgcgcg tcttgtcagg gctacggtga ttcatcggat gcgccatcca tgcggcgcat
    45601 ccaacaatga aaccttaccc gcgacgcgct ttcactgcgt tcgccagttg acgtaacaga
    45661 gcatcggtat cttcccagcc aatgcaggca tcagtgatgc tcttaccgta ggccagcggc
    45721 tccccgctct cgaggctctg attgccttcc accagatggc tttccaccat cacgccaata
    45781 atggcctttt cgccaccggc aatctgctgg caaacgtcag cacaaacatc catctgcttt
    45841 ttgaattgtt ttgacgagtt agcatggctg aaatcgatca tcacctgtgc tggcaggcct
    45901 gctttgttca gcccttcttt cacttcagca acgtgcttcg cgctgtagtt aggctcttta
    45961 ccgccgcgca gaatgatatg gcaatcaccg ttaccgctgg tattcacaat cgccgaatgc
    46021 ccccatttcg ttacggacag gaagcagtgc ggcgcgccgg cggcattaat ggcatcgata
    46081 gccactttaa tcgtaccgtc ggtgccattt ttgaagccga ccggacaaga aagccctgat
    46141 gccagttcgc ggtgcacctg cgattcggtg gtacgtgcgc caattgcgcc ccagctcatc
    46201 aggtcagcga gatattgtgg ggtgatcata tcgagaaact cacccgccgc tggcagaccg
    46261 ctgtcgttaa tatcaagcag caatttacgg gctatacgca gaccgtcgtt gatctggaag
    46321 ctgttatcca tatgcggatc gttaatcagc cctttccagc ccaccgtggt acgcggcttt
    46381 tcaaaataaa cgcgcattac gatttccagc tcatctttca gctcttcacg cagcgccagc
    46441 aagcgagtgg catactcttt agccgcgaca ggatcatgaa ttgagcacgg gccaatcacc
    46501 accaacaggc gatcatcatt acctttcagg atcttatgga tcgcttttcg ggcatgggca
    46561 accgtattcg cggcattttc agtagcgggg aatttttcca gcaatgcgac aggaggaagt
    46621 aactctttga tttctttgat gcgtaaatcg tcgttctgat aattcatgtc tgttccagtg
    46681 ttgccatact tatcttagtg aatgcaatac ttccaatcta tatcttccgt cagaatgtgt
    46741 aaacggggtt ttacactatg aacggattaa tcctggaatt agcaagaaaa acgccagatt
    46801 gtcgcgaaaa acgagatctc tcctacaatt tctaactgta actcctttgt ttgttaatta
    46861 tttcaagatt ctctgctgga tttctttgcc tggctgaaat cttaaaccaa tgccttatat
    46921 tcacctgcaa atgcactgtt ggaagaggtt atccgacata acgaccataa caggagcatc
    46981 ctatgaaaat gacaaaactg gccacactat ttctgactgc cactctaagc cttgccagcg
    47041 gtgccgcact ggctgccgat agcggagcgc aaaccaataa cggccaggca aacgccgcag
    47101 ctgatgcggg ccaggtagcc cctgacgccc gtgaaaatgt cgcgccaaat aacgtcgaca
    47161 ataacggggt aaataccggt tctggcggca ctatgctgca tccggatggt tcttcaatga
    47221 acaatgacgg catgaccaaa gatgaagagc acaagaacac catgtgcaaa gatggtcgct
    47281 gcccggatat caataaaaag gtacaaaccg gtgatggcat caataatgat gtcgatacca
    47341 aaaccgacgg taccacgcag taaccctgca aaatagccaa taatgggaga gcggagtctc
    47401 tcccttttaa tttttccctt ctaaaagcag tatgatgtca taaagttaag tgagaataat
    47461 taagggatga ctcgtcatgg cgcactcaca ctcacacacg tcctcacacc tgccagaaga
    47521 taataatgct cgtcgcttgt tgtatgcttt cggcgtgact gccgggttta tgctggtaga
    47581 agtcattggt ggttttcttt ctggttctct ggctttgctg gccgatgcgg gtcatatgtt
    47641 gaccgatact gccgccctgc ttttcgccct actcgccgtg caattttcac gtcgtcctcc
    47701 caccattcgc cacactttcg gttggctaag actgaccacg ctcgcggcgt ttgtgaatgc
    47761 catcgccctc gtggtaatta ccattttgat tgtctgggag gcgatagaac gcttccgcac
    47821 gccgcgtccg gtcgagggcg gcatgatgat ggcaattgcc gtggctgggt tgctggcaaa
    47881 tatactttct ttctggttac ttcatcacgg cagcgaagag aaaaacctca acgtacgagc
    47941 agcggcactg catgtgctcg gggacctgct gggttcggtt ggggctatta ttgccgcgtt
    48001 gattattatc tggaccggct ggacacctgc tgaccccatt ctctcgatac tggtgtcgct
    48061 tctggttctg cgtagcgcgt ggcgactgtt gaaagatagc gtgaatgaat tacttgaagg
    48121 tgcaccggta tcgctggata tcgctgaact gaagcgtcgt atgtgccggg aaatcccgga
    48181 agtccgcaat gtgcaccatg tgcatgtatg gatggtaggc gagaagccgg tgatgacgtt
    48241 gcatgtgcag gtgatcccgc cacacgatca cgatgccttg ttggatcaga tccaacacta
    48301 cctgatggat cactatcaga ttgagcacgc caccattcag atggaatatc aaccttgtca
    48361 tgggccagac tgccacctga atgaaggggt gtctggccat tcacatcatc accattaatg
    48421 ggacagcgcg cgtgagcctc tttcgcgtgc gctattgatc cacatccggc tgccgttgag
    48481 cgcaataaag gtcaggatga tgtactccag agacatggcg taaacgccct gaagtgcaaa
    48541 aataacgacg ctaatcacgt taataatcac ccacaacagc cagttttcca catacttacg
    48601 cgtcatcaga atcattgcca cgatagataa caccatcatg caggaatccc agaacgggaa
    48661 agcgtccggt tgcagttcag gcatcgcaac ctgtaatcct aatgcttgca tgatgatgac
    48721 tgccacgcgg gtcaaaaatg caaacaccgg attgataaat accgtcatca gaccaatagc
    48781 aatgacacaa accgccagcc aggttaatgc cttcggcaat ggtaaccagc gaattttcaa
    48841 ctccgcctcg ttctgactgg tttgtcgcga ccacgcatac caaccgtaaa tattcgcggc
    48901 aaagaaaaac acctgtaata gcaggctggc atacagctga atctgaaaga aaataatgcc
    48961 aaacaaggtg acgttgatca ggccaaagaa gtagttgctg atcttctcca gactggcaag
    49021 gccaatacac agcaacccgg cgatcgtgcc taccgcttcg atccatgaga gatcataacc
    49081 gcctgcccct attggtatat gtaccaggat attctgcaca ctaaaaaaat ccatctttcc
    49141 cccaaagcgt aacgttacgc cttattattc gttatccacg tagtgtagcc gcaaaatcca
    49201 gcatacgatt gagcggcacc agcgccctct ctcgcagcct ttcatcaaca tgaacctcgt
    49261 gattgcttcc ttcctgttct aatgcctcag cgatagcctg aaggccattc atggccatcc
    49321 acggacaatg cgcgcagctg cggcaggttg cgccctcacc cgcggttggt gcttccagta
    49381 actctttatc tggcaccgcc tgctgcattt tgtagaaaat gccccgatcg gttgccacga
    49441 taagccgctg atgtggcaat gttttcgcag cagcgatcag ttgactggtg gaaccgaccg
    49501 catccgccat atcgacaata gcttgtggtg attctggatg caccagtatg gcagcatccg
    49561 ggtattcttc ttgcaagcgg gttaacgcct gagtcttaaa ttcatcatgc acaatgcagg
    49621 caccctgcca gcataaaatg tctgcgcccg tctgtttttg cacgtaacgc cccagatgtt
    49681 tgtcgggtgc ccagatgatt ttttcaccca aactatcaag atgatcaata agttcgacgg
    49741 caatgcttga agttaccacc caatctgcgc gcgcttttac cgcagcagaa gtgttggcgt
    49801 agacgacgac agtacgatcg ggatgggcat cgcaaaatgc gttaaattct tcaacagggc
    49861 agccgagatc cagtgaacac tcagcctgaa gtgtcggcat cagaattgtt ttttccggac
    49921 tgagaatttt ggcggtttct cccataaatc tcaccccagc gactaacaac gtagaggcgg
    49981 gatgctttgc accgaagcgc gccatttcca aagaatcaga aatacagcca ccggtttctt
    50041 ctgccagttg ttgaatttcg ggatcggtat agtagtgggc aaccatcacc gcattacgtt
    50101 cttttagcag acgttttatc ttctcgcggt aatacgcttt ttcatcaatg cttaacggcg
    50161 tcggcttcgg ggggaaagga taaatcgccg tgtctggatc aaacatcacg ctcatcttac
    50221 catctcgttt tacaggctta acgttaaaac cgacattagc gtgatattcg ctgttatcgg
    50281 tgtaatttgt tttatatgct aaacaagata gtcgaatggt ggggaaaagt cactcgaatt
    50341 acgtatgaga tgccgaatgc cacactttgc gtcttattcc tccgacgtaa agttgtttac
    50401 gcagcaagcg cagagtatgt catcagggtc aagaattgaa attgagagat gcctgaatag
    50461 caaaaaagcg cctttagggc gattttttac attggtgggt cgtgcaggat gactcgcttc
    50521 gctcgccctt cgggccgtcg ccgcgagcgg ctccgttgtt tcgctacgct caactc
//
LOCUS       DAPGRN010000020        50064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPGRN010000020 DAPGRN010000000
VERSION     DAPGRN010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.020"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(128..415)
                     /locus_tag="RHW33_001295"
     CDS             complement(128..415)
                     /locus_tag="RHW33_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV1644818.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVEDEDIIRLVAER"
     gene            complement(490..633)
                     /gene="mgrB"
                     /locus_tag="RHW33_001296"
     CDS             complement(490..633)
                     /gene="mgrB"
                     /locus_tag="RHW33_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV1644819.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            792..1031
                     /locus_tag="RHW33_001297"
     CDS             792..1031
                     /locus_tag="RHW33_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644820.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1175..1966)
                     /gene="kdgR"
                     /locus_tag="RHW33_001298"
     CDS             complement(1175..1966)
                     /gene="kdgR"
                     /locus_tag="RHW33_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV1644821.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2143..3516
                     /locus_tag="RHW33_001299"
     CDS             2143..3516
                     /locus_tag="RHW33_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1644822.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACISGLRI
                     TQPRSRA"
     gene            complement(3565..4446)
                     /gene="htpX"
                     /locus_tag="RHW33_001300"
     CDS             complement(3565..4446)
                     /gene="htpX"
                     /locus_tag="RHW33_001300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV1644823.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4638..6686)
                     /gene="prc"
                     /locus_tag="RHW33_001301"
     CDS             complement(4638..6686)
                     /gene="prc"
                     /locus_tag="RHW33_001301"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV1644824.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6706..7404)
                     /gene="proQ"
                     /locus_tag="RHW33_001302"
     CDS             complement(6706..7404)
                     /gene="proQ"
                     /locus_tag="RHW33_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV1644825.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTAKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7501..7998)
                     /gene="msrC"
                     /locus_tag="RHW33_001303"
     CDS             complement(7501..7998)
                     /gene="msrC"
                     /locus_tag="RHW33_001303"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV1644826.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8128..9411
                     /gene="yebS"
                     /locus_tag="RHW33_001304"
     CDS             8128..9411
                     /gene="yebS"
                     /locus_tag="RHW33_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV1644827.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRSDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9380..12013
                     /gene="yebT"
                     /locus_tag="RHW33_001305"
     CDS             9380..12013
                     /gene="yebT"
                     /locus_tag="RHW33_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV1644828.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSFSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVINRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVGPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12093..13532
                     /gene="rsmF"
                     /locus_tag="RHW33_001306"
     CDS             12093..13532
                     /gene="rsmF"
                     /locus_tag="RHW33_001306"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV1644829.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAALPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAAQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13649..13885
                     /locus_tag="RHW33_001307"
     CDS             13649..13885
                     /locus_tag="RHW33_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV1644830.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13990..14181
                     /locus_tag="RHW33_001308"
     CDS             13990..14181
                     /locus_tag="RHW33_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV1644831.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14182..14838)
                     /gene="pphA"
                     /locus_tag="RHW33_001309"
     CDS             complement(14182..14838)
                     /gene="pphA"
                     /locus_tag="RHW33_001309"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV1644832.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALGKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLSERQKGQGITGADHFWFGHTPLRHRVDIGNQHYIDTGAVFGGELTFVQLQ
                     "
     gene            complement(15234..15575)
                     /locus_tag="RHW33_001310"
     CDS             complement(15234..15575)
                     /locus_tag="RHW33_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV1644833.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15588..16460)
                     /gene="copD"
                     /locus_tag="RHW33_001311"
     CDS             complement(15588..16460)
                     /gene="copD"
                     /locus_tag="RHW33_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV1644834.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16464..16838)
                     /gene="yobA"
                     /locus_tag="RHW33_001312"
     CDS             complement(16464..16838)
                     /gene="yobA"
                     /locus_tag="RHW33_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV1644835.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLSHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16977..17207
                     /gene="holE"
                     /locus_tag="RHW33_001313"
     CDS             16977..17207
                     /gene="holE"
                     /locus_tag="RHW33_001313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV1644836.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17309..17965
                     /locus_tag="RHW33_001314"
     CDS             17309..17965
                     /locus_tag="RHW33_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV1644837.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17988..18650
                     /gene="exoX"
                     /locus_tag="RHW33_001315"
     CDS             17988..18650
                     /gene="exoX"
                     /locus_tag="RHW33_001315"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV1644838.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKTWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18647..20707)
                     /gene="ptrB"
                     /locus_tag="RHW33_001316"
     CDS             complement(18647..20707)
                     /gene="ptrB"
                     /locus_tag="RHW33_001316"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV1644839.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20916..21575)
                     /locus_tag="RHW33_001317"
     CDS             complement(20916..21575)
                     /locus_tag="RHW33_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV1644840.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(21902..22258)
                     /locus_tag="RHW33_001318"
     CDS             complement(21902..22258)
                     /locus_tag="RHW33_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV1644841.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22325..22615)
                     /gene="yebG"
                     /locus_tag="RHW33_001319"
     CDS             complement(22325..22615)
                     /gene="yebG"
                     /locus_tag="RHW33_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV1644842.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22749..23927
                     /gene="purT"
                     /locus_tag="RHW33_001320"
     CDS             22749..23927
                     /gene="purT"
                     /locus_tag="RHW33_001320"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV1644843.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAERVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23983..24624)
                     /gene="kdgA"
                     /locus_tag="RHW33_001321"
                     /gene_synonym="eda"
     CDS             complement(23983..24624)
                     /gene="kdgA"
                     /locus_tag="RHW33_001321"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV1644844.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24661..26472)
                     /gene="edd"
                     /locus_tag="RHW33_001322"
     CDS             complement(24661..26472)
                     /gene="edd"
                     /locus_tag="RHW33_001322"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV1644845.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26707..28182)
                     /gene="zwf"
                     /locus_tag="RHW33_001323"
     CDS             complement(26707..28182)
                     /gene="zwf"
                     /locus_tag="RHW33_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV1644846.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28520..29389
                     /gene="hexR"
                     /locus_tag="RHW33_001324"
                     /gene_synonym="yebK"
     CDS             28520..29389
                     /gene="hexR"
                     /locus_tag="RHW33_001324"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV1644847.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29517..30959
                     /gene="pyk"
                     /locus_tag="RHW33_001325"
     CDS             29517..30959
                     /gene="pyk"
                     /locus_tag="RHW33_001325"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV1644848.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31090..32061)
                     /gene="lpxM"
                     /locus_tag="RHW33_001326"
                     /gene_synonym="msbB"
     CDS             complement(31090..32061)
                     /gene="lpxM"
                     /locus_tag="RHW33_001326"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV1644849.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQLRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32180..33502)
                     /gene="mepM"
                     /locus_tag="RHW33_001327"
     CDS             complement(32180..33502)
                     /gene="mepM"
                     /locus_tag="RHW33_001327"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV1644850.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33518..34450)
                     /gene="znuA"
                     /locus_tag="RHW33_001328"
     CDS             complement(33518..34450)
                     /gene="znuA"
                     /locus_tag="RHW33_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV1644851.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDEDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34529..35284
                     /gene="znuC"
                     /locus_tag="RHW33_001329"
     CDS             34529..35284
                     /gene="znuC"
                     /locus_tag="RHW33_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV1644852.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35281..36066
                     /gene="znuB"
                     /locus_tag="RHW33_001330"
     CDS             35281..36066
                     /gene="znuB"
                     /locus_tag="RHW33_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV1644853.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(36215..37225)
                     /gene="ruvB"
                     /locus_tag="RHW33_001331"
     CDS             complement(36215..37225)
                     /gene="ruvB"
                     /locus_tag="RHW33_001331"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV1644854.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37234..37845)
                     /gene="ruvA"
                     /locus_tag="RHW33_001332"
     CDS             complement(37234..37845)
                     /gene="ruvA"
                     /locus_tag="RHW33_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV1644855.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37984..38049)
                     /gene="yobI"
                     /locus_tag="RHW33_001333"
     CDS             complement(37984..38049)
                     /gene="yobI"
                     /locus_tag="RHW33_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HDV1644856.1"
                     /translation="MYIFITHFFTEYVILKYLLPI"
     gene            38120..38722
                     /locus_tag="RHW33_001334"
     CDS             38120..38722
                     /locus_tag="RHW33_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV1644857.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38724..39245)
                     /gene="ruvC"
                     /locus_tag="RHW33_001335"
     CDS             complement(38724..39245)
                     /gene="ruvC"
                     /locus_tag="RHW33_001335"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV1644858.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39280..40020)
                     /locus_tag="RHW33_001336"
     CDS             complement(39280..40020)
                     /locus_tag="RHW33_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1644859.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40049..40501)
                     /gene="nudB"
                     /locus_tag="RHW33_001337"
     CDS             complement(40049..40501)
                     /gene="nudB"
                     /locus_tag="RHW33_001337"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV1644860.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40619..42391)
                     /gene="aspS"
                     /locus_tag="RHW33_001338"
     CDS             complement(40619..42391)
                     /gene="aspS"
                     /locus_tag="RHW33_001338"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV1644861.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42701..43267
                     /locus_tag="RHW33_001339"
     CDS             42701..43267
                     /locus_tag="RHW33_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1644862.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43264..44082
                     /locus_tag="RHW33_001340"
     CDS             43264..44082
                     /locus_tag="RHW33_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV1644863.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44135..44530
                     /locus_tag="RHW33_001341"
     CDS             44135..44530
                     /locus_tag="RHW33_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV1644864.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44571..45314
                     /gene="cmoA"
                     /locus_tag="RHW33_001342"
     CDS             44571..45314
                     /gene="cmoA"
                     /locus_tag="RHW33_001342"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV1644865.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45311..46282
                     /gene="cmoB"
                     /locus_tag="RHW33_001343"
     CDS             45311..46282
                     /gene="cmoB"
                     /locus_tag="RHW33_001343"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV1644866.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(46447..48876)
                     /gene="torZ"
                     /locus_tag="RHW33_001344"
     CDS             complement(46447..48876)
                     /gene="torZ"
                     /locus_tag="RHW33_001344"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV1644867.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(48901..50001)
                     /locus_tag="RHW33_001345"
     CDS             complement(48901..50001)
                     /locus_tag="RHW33_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV1644868.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGFLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        11858 a        12988 c        12304 g        12914 t
ORIGIN      
        1 ataatatgat aattatttga agtcagacca gagttttaat ttgaagcagt tgcaccatga
       61 cagtgcgtat actcaggatg aattgagtaa cccttcaacg acggggttac tcaagattga
      121 ggaaggatta tcgttcggcg accaggcgaa taatatcttc gtcttcaacc gatttttgac
      181 tctcaatggt ctttttatct gctttctctg gttcgtttgc ttcgcacaaa cgacgtaaca
      241 gtgcattttg acgtttttgc tgatccagca acgcctcaag cagttcaatc tgttcgttag
      301 tccgagaact ggcacgatta ataaaaaacc acaagatgag cccgataaga agaaccacca
      361 ccgatacaac caaagacgca atgttcatca cgcctgaatt tacaacttcg ttcatttcac
      421 cacctctgag tagaggcgct attctaccac tgctggagag gaagaaaatc tagtgctgaa
      481 aaaatgatat caccacggga taaactggtt aatggcacaa attccgctga aaaattgtac
      541 atcctgatcg cacatcatgt tgaatacctg cgcccaaagc agcaagcaag ccaacaccac
      601 gacaaccaga acgacccatc gaaacttttt cactccaatc tccgtttcac ctaccttatg
      661 tcattaaact agcatgcgtt agttaaacag cgcctaacta tgtcgatatt cgtgcttttt
      721 cggaatgagt cgcttgcttc agcgcatatt gccgctacga ttaagcgaac ataaaaagag
      781 aagaggttgt aatgcgattc atcattcgca cagttatgct gatagcactg gtatggatag
      841 gattattact cagcggatat ggcgttttga ttggaagcaa agagaatgcc gcaggattag
      901 gtttgcaatg tacctatctg actgccagag gcaccagcac ggtacaatac ttgcacacta
      961 aaagtggttt tttggggata acagattgcc cgctgttacg caaaagcaat atcgtggtcg
     1021 ataatggtta agtgtaataa ataaccagat tctcactcat gagtaaaata ctcttatagc
     1081 aagcgcgttt aatataacgc caccttcaac tttatttttc tcatgctaaa taaagaacct
     1141 gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg tgataaccca
     1201 tttgtgcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc tgtaaacgct
     1261 cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc acgccaaagc
     1321 gatcaaatac cggtaccgca atgcatcgca gtccttcttc ctgctcttcg ttatcttcgc
     1381 catacccctg ctcgcgcacc tggtccagaa ccggtaataa cgcttctgta ctggtgatgg
     1441 tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact tcatcgcgat
     1501 cgcgccatgc cagcagcacc ttaccaatcg cggtgctgta cagcggatta cgacgcccaa
     1561 tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa acaatactgt
     1621 cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc tcacgcatct
     1681 ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc agttcaaaca
     1741 atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa cctaaggttt
     1801 tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc tgcgacagct
     1861 cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa acttttagca
     1921 cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat cacctcattg
     1981 cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag ggtggtcgca
     2041 atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat cagtgctaga
     2101 atcatacccc tgttgattat tcactaaaga tataaaattc ctatgccaaa agttcaggcc
     2161 gacggcctgc cgttgcccca gcgatacggt gcgatattaa ccattgtgat tggtatttcg
     2221 atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaatcgc cacggacctt
     2281 catgccacgc cagccagttc catctgggta gtgaacgcct atcaaatcgc cattgtcatc
     2341 tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat ttataaatgc
     2401 ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc gctgcaaatg
     2461 ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag cgttaatacc
     2521 gcacttatcc gcctgatcta tccacaacgt tttctgggta gagggatggg cataaactcg
     2581 tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat cctctccatc
     2641 gcatcctgga aatggttatt tttaatcaac gtaccgttgg gtattatcgc cctgcttctg
     2701 gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt cgacctgccc
     2761 agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag tggtttcgct
     2821 caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt cgttggtatt
     2881 ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga tttactgcgt
     2941 atcccgctgt tttcactttc tatatgcaca tctgtttgct ctttctgcgc acaaatgctg
     3001 gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca
     3061 ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctccgtt ggcaggctat
     3121 ttgattgaac gcgtacatgc aggattgctg ggtgctttag gattattcat catggctgcg
     3181 gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat tatctggccg
     3241 atgatcttat gtggcgctgg atttggattg ttccagtcac ccaataacca caccattatt
     3301 acctccgctc ctcgcgaacg tagcggtgga gccagtggca tgttaggaac ggctcgtcta
     3361 ctgggtcaga gtagcggcgc ggcgctggtg gcgctgatgc taaatcagtt tggagataat
     3421 ggtacacacg tctcgctgat ggctgcggct attctggcag tgattgctgc ctgtatcagt
     3481 ggtttacgta tcactcagcc acgatccagg gcataaaaaa agcgcgtcga tcaggacgcg
     3541 ctttttagtg tttacttcat aatattactt caggtattca cccgtacgca gagcttcaat
     3601 tcgtttatcc agcggcgggt gagtcatgaa caactcactg agcgatttcg acttaccgtt
     3661 aatgcagaaa gccatcatgc tggttgcttc ttgcggttca tagctggttt tcaggcgctg
     3721 cagcgcggca atcattttct cgcgaccaac cagttttgcc gaaccggcat cagcatggaa
     3781 ttcacgatga cgcgagaacc acatggtgat aatgctcgcc agaataccaa acaccagttc
     3841 cagaaccgtt gcaaccgcaa agtagatcag cgggttgccg ttgctctctt caccttcatc
     3901 acgatttccg cccataaaac ccgcggcaag ctgcgccagg atacgggaaa taaagataac
     3961 gaaggtgttc accacgccct gaatcagcgt catggtgacc atatcaccat tggcgatgtg
     4021 gctgatttcg tgagcaatta ccgcctcggc ttcatccggg ctcatgttct gcagcaaacc
     4081 ggtgctgaca gcaaccagag aggcatcacg gcgcgcaccg gttgcaaaag cgttgatgtc
     4141 cggcgcatgg tagatagcca cttgcggcat agcgatcccc gcctgacgag cctgggttgc
     4201 tacagtattg accagccaac gttccctttc gttacgcggt tgctcgatca cttccccgcc
     4261 aacagatcgt aatgccatcc atttggacat cagaagcgaa acgaaggaac caccaaaacc
     4321 gaacagcaag gccatgatca tcagcccctg aacgctgctc gactgtatcc ctgtcaggct
     4381 cagtaccagc ccgaaaacga ccattacggc caggttcgtt agcaggaaga gcgcgattcg
     4441 catcataatt ttctttttac ctcagtttaa caaaacgcaa tatgcgatac ccacatcgta
     4501 tgggttacgc gactattttc aagtctggat agtgcgtaag tcaccagaaa gacacaactt
     4561 tacattttgt agcatctgat ttacggcatc ttgccgctgt taaaaaatca ggcacaattt
     4621 cttgtgcctg attgatatta cttgacgggt gcgggttgtt ccgcgggtct ggctttttca
     4681 agcttcgcca gatcgagtgc gatattcacc gtctcatcca gataaggatc cggctcctgg
     4741 taatctttcg gtagatcatc cagtttcttc aactccggtt taccttcgcg tttaaagcgt
     4801 tcgttcaaac gcgccagacg cgtcgcatca tcttcattat tctctttctc acgcacagcg
     4861 taattcagag aaacgatatt gcgcttgtcc ttcatagcgt tgaagcgcgc gatatccttc
     4921 atgatgttct ggaactcagg atctttcgcg atacgcgcat tatgttcctt cagtagctcc
     4981 ggttcaaagg ccgttaaatc tcctgatttc acataagtcg cggcatcaat gctatcccac
     5041 ggcagcgcgt tatcttcgaa tttctcaccc gtttccgttt cttcattacc cgtcggcatg
     5101 atgatgtctg gcgttacgcc tttacgttgc gtactgccac cgttaacgcg atagaatttc
     5161 tggatcgtgt actgcacaga acccagcgct ggccattcag gacgtaacat ctgatcgtaa
     5221 atacggttca atgaacggta ttgctgaacg gtgcctttac caaacgtcgg ttcacccaca
     5281 accagcgcac gaccgtaatc ctgcattgcc gcggcaaaga tttctgaagc cgaagcactg
     5341 aagcggtcaa ccagtaccac cagcgggcct ttatagaaaa cctgtccgtc ggtatcgctg
     5401 tcttcacgaa ctttaccgtt gttatcacgg acctgaacaa taggacccgc aggaataaac
     5461 agacctgaga gcgataccgc ttcggtcaac gccccaccgc cattgctacg caggtcgatg
     5521 atcacactgc tgacattctg tttttccagt ttctgcagtt gtactttgac atcgtctgtc
     5581 aaacccacat agaagcccgg aatatccagt acgccgactt tctctttacc gacggtcttc
     5641 accgacattt taaccgcgcg gtcttcgaga cgaatacgtt cacgggtcaa tgttacagtg
     5701 cgggtcttgg tccctttacc agcaggtaaa atttccagac gaactttact gcccttcggc
     5761 cctttaatta aggcaaccac atcatcaaga cgccagccaa tcacgtcaac catcggcttg
     5821 cctgtttgac caacaccgac aattttatca ccaacgctga tagctttact cttcgctgcc
     5881 ggaccacctg ccaccatcga attgataacg gtgtagtcat catccatttg cagcactgcg
     5941 ccaatacctt ccagcgacaa actcatttca gtgttgaact gttcggtatt acgcggggaa
     6001 agatagttgg tatgcgggtc gatttcacgc gcaaacgccg tcattgccag cgagaaaaca
     6061 tcttcgctgt tggtttgcgc cagacgacga atggcaaatt tgtagcggcg agtcagggtt
     6121 tcacgaattt ctttatccgt ttttcctgcc agcttcaggc ttaactcgtc gaatttgact
     6181 ttactgtccc acagcgcgtt caactcagcc tcgtttttcg gccagggcgc tttgctgcgg
     6241 tcaaggttat aagtgtcgtt gccggtgaaa tccatcggct tttccagtac cgacaaagcg
     6301 tactggtaac gctcaaaacg gcgcttttgc gccagattgt agagatcgta gaaaacgtcg
     6361 agtttgcctg aacgcagttc atcgcctaac tcggttttct ttttcgcgaa ctgttcaaca
     6421 tcgcttgcca acagcacgtt gtggctgtaa tcgagcagat tcaggtagcg gtcaaagatt
     6481 ttggccgaaa atgcctgatc gaggtcgaac tggcgataat gagaacgggt gaagcgcgac
     6541 gttacgcgct cactcaccgt cgcatgctgc gtctcttcct ttaataccgg aatttgatca
     6601 gcacgcgtga tatcttctac agcgaaggtc tggcctgcta ttgcaagcag gccagctaac
     6661 gcggtaagcc taaaaaacat gttcatgcct ggcccggcct ccgtttcaga acaccaggtg
     6721 ttctgcgcgc acaatcaaag acatacccga attcagctgg acgcggacgc cgtctttggt
     6781 gatttctaat acggtggcat ccatcgcgtt ttgacccgct ttcaccttca gggcttgtcc
     6841 gacagtcaga gctgaaatgt cagaaactgg ggtgtgctgt tcttcgcgag gtgcttttgc
     6901 tgtttttggc gctttctcta ccggcttttg cgcacgaggt ttacgttcag cgccttcttt
     6961 gcggcgtggc gtggtcggac gtggcttgcg ttcgcggcgc ggtgcgtctt ctttctcacc
     7021 agcagctgcg gcagcttcgc gttttttcgc ttgctgttca gcacgctgtg cctgaacacg
     7081 cgctttcgct tcttcaagct gcttgcgagc atgctctaca tgttgctcgt ccagctcacc
     7141 gcatgggttg ccgtcaagat cgacacgcgt tgcgccgggt ttaacaccgt aaaggtaacg
     7201 ccagctcgaa gtgtagagac gtaaagcgga tcgtaattgc gttttgctca ggttcatttc
     7261 cccggcaaca cgatcgacca aatcctgaaa aataccgatt ttcagcggac gcgcttcacc
     7321 ttccgcactg aaacagtggg gaaaacgttc ggccagaaac gcgattactt ctttactgct
     7381 attcaactta ggttgatttt ccatgaaatt tcctgattac aacggacgta gccaacaagc
     7441 gcaggcatga acaggcgtca ttataatgac gctatcagta aatgctacgt tatccgttga
     7501 ttatcctgcg acgctcgcaa agaatttttt gtaatccgtc gttgcaagca ctttttcaag
     7561 ctgtgccaca agctgacgta agccttgctc gtcctcgtct gtaaagcgac cgaagacggt
     7621 actatcgatg tcgagaacac caataatctg atttttcacc accagcggca gaacaatttc
     7681 agagttactc gccgcatcac aggcaatatg cccgtcaaac gcgtgaacat cctcgatacg
     7741 ctgcacttga ttgcgggcaa ccgcagtgcc gcacacgccg cgcccgacgg gtatccggac
     7801 acaggcaatt ttgccctgaa atggtccgag taccagtgta tcgtcctcaa gcaaataaaa
     7861 acctgcccag tttacgtcag tgagacgctc atataacaac gcactggtgt tcgcaagcgt
     7921 tgccagaaaa ctggtttctc ccgccatcag cgcgttaaag tcgcgattta aatccgcgta
     7981 aaattctgtt ttgttcatta tataatcact tggttgtctt acctggaact gccagcctat
     8041 taaaataagc attaaatgcg ttaatgctca agatcattcc catcatgggt taagattaat
     8101 gttaattctt attacatttg gcacgtcatg gctcttaaca caccacaaat tacgccgaca
     8161 aaaaagataa cagtgagggc aatcggcgag gaactgccgc gtggtgatta ccaacgttgc
     8221 ccgcaatgtg acatgctgtt tagcctgccc gagataaatt ctcatcaaag tgcctattgt
     8281 ccgcgctgtc aggcaaaaat tcgtgacggg cgcgactggt cgctaacgcg cctggcggca
     8341 atggccttca ccatgctgtt gctgatgccg tttgcctggg gcgaaccgct gttgcatatc
     8401 tggctgttag gtattcgtat cgacgccaat gtcatgcaag gcatctggca aatgaccaaa
     8461 cagggcgata cgataacggg ggcgatggtc tttttctgcg ttattggtgc ccccctcatt
     8521 ctggtgtcct ccatagcata tttatggttt ggtaaccgac tgggaatgaa tttacgtccg
     8581 gtactgctga tgcttgagcg acttaaagag tgggtaatgc tggatatcta cctggtcggc
     8641 attggcgttg cttctataaa ggtacaggat tatgcccata tccaggcggg tgtcggcttg
     8701 ttctcttttg tggcgttggt gattttaacg acggtgacgt tgtcacatct taatgtcgaa
     8761 gaactgtggg agcgatttta tccgcagcgc cccgctacgc gtagtgacga gaaacttcgt
     8821 gtctgtcttg ggtgccattt taccggctat cctgatcagc gtggtcgctg cccgcgttgc
     8881 catattccgc tacgcctgcg tcgccgtcat agtttgcaaa aatgctgggc ggcgctgtta
     8941 gcgtcaatcg ttttgttgtt acctgccaac ctgttgccta tttctatcat ttatctgaat
     9001 ggtggccggc aggaagatac gattctttcc gggattatgt cgctggcaag tagcaacatt
     9061 gccgttgcgg gaatcgtgtt tatcgccagt attctggtac cgtttactaa agtgatcgtc
     9121 atgttcactt tactgttgag cattcatttt aaatgccagc aaggtttacg cacacgcatt
     9181 ctgttactgc gaatggtgac ctggattggt cgctggtcga tgctcgacct gtttgtcata
     9241 tctttaacca tgtcgctgat taatcgcgat cagatcctcg cttttactat gggaccggct
     9301 gcgttttatt tcggcgcagc ggtaattttg actattcttg ctgtggaatg gctggacagc
     9361 cgcttacttt gggatgcaca tgagtcagga aacgcccgct tcgacgactg aagcgcagat
     9421 taaaaataaa cgccgtatct cacctttttg gctgctgccg ttcatcgcgc taatgattgc
     9481 cggttggctg atttgggaca gttatcagga ccggggtaat accgtcacca tcgactttat
     9541 gtcggcggat ggtattgttc caggccgtac gcctgttcgt tatcagggcg ttgaagtcgg
     9601 aacagtgcag gatatcagcc tcagcgacga tcttcgtaag attgaagtca aggtcagcat
     9661 caaatccgat atgaaagatg cgctgcgcga agagacacag ttctggctgg tgacgccaaa
     9721 ggcctcgttg gcaggtgtct ccgggctgga cgccctcgtc ggtggtaact atatcggcat
     9781 gatgccaggt aaaggtaaag agcaggatca ctttgtcgca cttgataccc aaccgaaata
     9841 tcggctggat aacggcgatc tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa
     9901 cagcggttca ttggtctatt tccgcaagat cccggtggga aaagtctacg actatgccat
     9961 caatcccaac aagcaaggcg tggtgattga tgtcctgatc gagcggcgtt ttaccgacct
    10021 ggtgaaaaaa ggtagccgtt tctggaacgt ttccggcgtt gatgccaacg tcagtatcag
    10081 tggcgcgaag gtgaaactgg aaagtctggc ggcactggtt aacggtgcga ttgccttcga
    10141 ttcacctgaa gagtcgaaac ctgccgaggc ggaagatact tttggtctgt atgaagatct
    10201 ggcccacagc cagcgtggcg taataataaa actggaactg ccgagtgggg ccggattaac
    10261 cgccgactcg acgccgttaa tgtatcaggg gctggaagtc ggacagctaa ctaaactgga
    10321 tttaaatcct ggtggtaaag tcactgggga aatgaccgtt gatcccagcg tcgttaccct
    10381 gcttcgtgaa aatacccgca tcgaattacg taatccgaaa ttatccttta gcgatgccaa
    10441 tctcagcgcc ctgctgactg gcaaaacctt cgagttggta cccggcgatg gcgagccacg
    10501 caaagagttc gttgttgtgc caggcgaaaa agcactgctg catgaacctg atgttctgac
    10561 gctgaccctg accgcgccgg aaagttacgg tattgatgcg ggtcagccgc tcattcttca
    10621 cggcgtgcag gtaggccagg ttatcaatcg taaactcacc agcaaaggcg tcacctttac
    10681 cgtcgccatc gagcctcagc atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa
    10741 cagccgtgtc gatgtgaagg tggggctgga tggcgttgag tttctcggtg ccagcgcctc
    10801 agaatggatt aacggcggga tacgtattct gccgggcgat aaaggcgaga tgaaagccag
    10861 ctatccactg tatgccaatc tggaaaaagc gctggagaac agccttagcg atttacccac
    10921 cacaaccgtg agtttgagtg cagagacgct gccggatgtg caggcaggat cggtagtgct
    10981 gtaccgtaaa tttgaagttg gtgaagttat taccgtgggt ccgcgagcta acgcgtttga
    11041 tatcgatctg catattaagc cggagtatcg caaccttctg accagcaata gcgtgttctg
    11101 ggcagaaggc ggggcgaaag ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc
    11161 gctctccaga gcattaaagg gagccattag cttcgataac ctcagcggtg ccagcgccag
    11221 tcagcgtaaa ggcgataagc gtattctgta tgcttccgaa acagcggccc gtgcggttgg
    11281 cgggcagatt acgcttcacg ctttcgatgc cggaaaactg gcggtcggga tgcctattcg
    11341 ctatctcggt attgatatcg ggcaaatcca gacgctggat ctgattaccg cgcgcaatga
    11401 agtacaggca aaggcggtgc tctatccgga atatgtccag acctttgctc gcggtggtac
    11461 gcgcttctca gtggtcacac cgcaaatttc ggcagctggc gttgagcatc ttgatactat
    11521 cctccagccg tatatcaacg tcgaaccagg ccggggcaat cctcgccgcg actttgaatt
    11581 acaagaggcc accattactg attcgcgtta cctggatggc ttaagcatta ttgttgaagc
    11641 gccggaagcc ggttcgttag gcatcggtac gcctgtgctg ttccgtggtc tggaagtcgg
    11701 tacggttacc ggaatgacgc tggggacatt gtcagatcgc gtgatgattg cgatgcgcat
    11761 cagtaaacgc tatcaacacc tggtgcgtaa caattccgtc ttctggttgg catcgggtta
    11821 cagtctggac tttggtctga cgggcggcgt agtgaaaacc ggcaccttta accagtttat
    11881 ccgtggcggc atcgccttcg ccacgcctcc gggtacgcca ctggcaccga aagcccagga
    11941 aggcaagcac ttcctgttgc aggaaagtga accgaaagag tggcgtgaat ggggaactgc
    12001 gcttcccaaa taatgcccac tgctccggcg tgcctgcgcc ggagcgttta tgctaaactg
    12061 cgcgcctgtt tttttgccag tggtatatgc tcgtggccca acacaccgtt tatttcccgg
    12121 acgcctttct gacgcaaatg cgcgaagcga tgccttcgac gctctctttt gatgattttc
    12181 ttgctgcctg tcagcgcccg ttgcgccgca gcattcgcgt taatacgctg aaaatctccg
    12241 ttgctgattt cctgcaatta accgctcctt atggctggac gcttacgcca attccgtggt
    12301 gtgaagaagg tttctggatt gaacgcgaca atgaagatgc attgccattg ggtagtaccg
    12361 ccgagcattt aagtggcctg ttttatattc aggaagccag ttcaatgttg cccgtcgccg
    12421 ccttgtttgc tgacggtaat gcaccacagc gggtgatgga tgtcgctgct gcgccaggct
    12481 ccaaaacgac gcaaattgcc gcgcggatga ataacgaagg ggcaatcctt gccaatgagt
    12541 tttccgccag tcgggtaaaa gtgttacatg ccaatatcag ccgctgtggt atcagtaatg
    12601 ttgcgctcac acattttgat ggccgcgtgt ttggtgcggc attgccagaa atgttcgatg
    12661 ccattttgct ggacgctccc tgctccggcg aaggcgtggt gcgtaaagat cccgatgcgc
    12721 taaaaaactg gtcaccagaa agcaatcagg aaatcgcagc tacacaacgg gagcttatcg
    12781 acagcgcctt tcatgcatta cgtcctggtg gtacgctggt ttactcgacc tgtaccttaa
    12841 accgggaaga aaacgaagcc gtttgcctgt ggcttaaaga gacttaccct gacgcagtag
    12901 agtttttacc acttggcgat ctcttccctg gtgcaaacaa agcgctgacc gaagaaggct
    12961 ttttgcatgt tttcccacaa atttacgact gcgaaggctt cttcgttgct cgtctgcgta
    13021 aaactcaggc cattcccgcc ttacccaccc caaaatacaa agtgggcaat ttcccgttca
    13081 gcccggtgaa agatcgcgaa gccgcacaaa ttcgtcaggc ggctgcaggt gttggcttaa
    13141 actgggatga aaacctgcgc ctctggcagc gtgacaaaga actgtggttg ttcccggtgg
    13201 gcattgaagc cctgatcggt aaagtccgat tttctcgcct ggggattaaa cttgccgaaa
    13261 cgcacaacaa aggttatcgc tggcagcatg aagcagttat tgcccttgcc acccccgaca
    13321 atgtgaacgc ttttgaactg acaccgcagg aagcggagga gtggtatcgc gggcgcgatg
    13381 tttacccgca agccgcgcca gtggcggatg acgtgttggt tactttccag catcagccga
    13441 ttggtttagc caaacggatt ggttcgcgac tgaaaaacag ctacccgcgt gaactggtgc
    13501 gcgatgggaa actttttacc ggtaacgcct gacagcgcac aaaaaaacgc actttttgac
    13561 tggcacattc ggctgcctca actaggctga aaaatggtgc gatcggactg gtcgtaccac
    13621 aaccggcagc taaatggaga gcacgaagat gaaaaccagt gtgcgcatag gcgcttttga
    13681 aatcgacgac ggcgaattac acggcgaatc gccgggtgat cgaacgttaa ccattccttg
    13741 taaatctgac cccgatttat gtatgcaact ggatgcctgg gatgctgaaa ccagtatccc
    13801 tgccctgcta aatggcgaac actctgtcct ttaccgtacc cgttacgatc aacaatctga
    13861 tgcctggatt atgcgtcttg cctgatccaa aaagaacccg tcggcatggc gggttatttg
    13921 tcctggttat tcccccgttg taaaatctct cctaaactta acggtacggc accacacttc
    13981 ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc tggatggcgg ttgtgaagat
    14041 attgttgtgg atgtctacaa cacggaacag cagtgtcttt attctatgag cgatcaacgg
    14101 atccgccatg gcggttgttt tccgattgag gattttatag atggtttctg gcgaccagca
    14161 caggagtatg gtgattttta attattgcaa ttgcacgaaa gtcagttcgc ccccaaagac
    14221 agcaccggta tcaatataat gctggttgcc aatatccacg cgatggcgca acggtgtatg
    14281 accaaaccag aaatgatcag cacctgtaat tccctgccct ttttggcgtt cacttaatcg
    14341 cgagcggctc cacaagacct gatgcaaatc aacgtccttt tgccattcat aaacatcatc
    14401 tggataatcg gcatgagcaa taacatgttt gccggtgcgg ctgtgtactt caagaataaa
    14461 gggcaaatgc tgacatttcc ccagcgccgt tttcgcttgt ttctgttgat tatctgccag
    14521 cgcaataaac cagtcgccgc cattcatcaa ccataaagac atctgctggg atgccagcgc
    14581 atccatcgcc atctgttcat gattgcctct taccgcacga acccaatgtt gttccagtaa
    14641 ctgcagacaa cgtaaacttt gcggcccacg atcgataacg tctcccactg agataagtaa
    14701 atctcgccac ggatcaaaac gacaatgcca taatttgcgg cgcaactgct caagacaacc
    14761 gtgtatatcg ccagaaagcc agatatgtcg ccattgatga cccgcaattc tctgataaac
    14821 gggcgcaggc tgtttcatca atattttcct cccgcgctaa agatcacata atcttaacaa
    14881 gaatgttaaa aaatgctgga ctcagacagt agagtgtgtg ttatggttga ctataaagtc
    14941 agcgaaggaa atgcttctgg cttttaacag ataaaaagag accgaacacg attcctgtat
    15001 tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag
    15061 gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca
    15121 aaatggtcaa taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg
    15181 tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag
    15241 agtttttcag cccgttctat aaacggtgcc agactcattt tttcgccggg attgttggga
    15301 tcatcaatct gaatcaccga aatgggttgg gctttagtct tcccactggc aacttccttc
    15361 tgtgcgatat cgtttaaagg atactgcacg agggtacttg ggttaatgac atacaaagca
    15421 ttacccggtc tgcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc
    15481 acttcaaaac ggctgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg
    15541 agtaacagaa acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg
    15601 agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta
    15661 gtcatccaca caaaccaggg tgccgcacga tcttcatctt gcctcatgcg cggtacaaga
    15721 acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat
    15781 aagagttggc cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta
    15841 agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga aaaacgcatc
    15901 agcgcctgaa tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc
    15961 ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga
    16021 tggattttcg ccgttacccc ttcattcagc gtcgcatggc cgactcccgc cagcagaata
    16081 aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg
    16141 acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt
    16201 tgcaaaacag cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac
    16261 ccgccctgaa cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt
    16321 tgtaaacgca gaaaacgctt cgccagcaaa cgacgaatcg ttaagggtgc cagccaggca
    16381 ccgtacattg cgaagccaaa gaccagcatc agcgaggtga aatggataaa tcgcagcgcg
    16441 atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta
    16501 tgcccgtcca ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg
    16561 gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta
    16621 atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc aacaccttcc
    16681 gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac
    16741 tgatgcgata aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt ggtcaggatt
    16801 gccagcgcgt agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat
    16861 aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct
    16921 tgccatccag gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc
    16981 tgaagaatct ggcaaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg
    17041 cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac
    17101 gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg
    17161 cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacagag
    17221 ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat
    17281 ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata
    17341 tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat catctggagt ttgtgagggc
    17401 cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga
    17461 ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta
    17521 tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg
    17581 ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca
    17641 gagtcatggt gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc
    17701 gcaaggtatg gatatggacc cgacctgttc actgtttacg acagggcaat gtctggggga
    17761 acccgaccta ctggcatcta cccgccgctt acaatttttt tcacatcagt actcgattgc
    17821 cgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat
    17881 cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca
    17941 aggcgatatc attccattac gctaggcttt tcggcctgga gcatgccatg ttgcgcatta
    18001 tcgatacaga aacctgcggt ttgcagggag ggatcgttga gattgcctct gttgatgtca
    18061 ttgacggaaa aatcgtcaac cccatgagcc acctggtgcg ccccgatcgt cctattagtc
    18121 cacaagcgat ggcgattcat cgcatcaccg aagccatggt cgccgataaa acgtggattg
    18181 aagatgtgat cccacactat tacggtagtg aatggtatgt cgcgcataac gccagctttg
    18241 accgccgcgt actgcctgag atgcctggtg agtggatttg cactatgaaa ctggcccgtc
    18301 gtttgtggcc agggatcaag tacagcaata tggcgttata taaaacacgc aagctcaatg
    18361 tacagacgcc gccgggcctg catcatcacc gcgcgttgta tgactgttat atcaccgccg
    18421 cgttgcttat cgatattatg aacacctccg gctggacggc agaacagatg gccgatatca
    18481 ccggacgtcc gtcgttgatg acgaccttca cctttggcaa ataccgtggc aaagcggttt
    18541 ccgacgttgc cgaacgcgat ccgggctatc tgcgctggtt atttaataac ctggacagca
    18601 tgagcccgga gctgcgttta acactgaaac attatctgga aaatacttaa tccgcaggcg
    18661 tagcgggtaa tgttccctgc gccagcgcga ccagaaaagc atattccatc gctacgcctt
    18721 cgtacgattt aaagcgacca gatttaccgc catggcctga gtccatgtcg gtacagagca
    18781 ataaaagatg gttatcggtt ttcagctcgc gcaacttagc gacccatttt gccggttccc
    18841 aatattgcac ctgagaatcg tgtaaaccgg tcgttaccag taaatgtgga taagcctgtg
    18901 cggtgacgtt gtcatacggg ctgtagcttt tcatatactc gtaatattgc ggatcctgcg
    18961 gattccccca ctcttcaaac tcaccagtgg taagaggaat tgattcatca agcatcgttg
    19021 ttacaacatc aacaaacggt acctgggcga taacgccgtg gaataattcc gggcgttgat
    19081 taattgcaac gcccatcaac atgccccccg cactcccgcc catcgcataa caaagcgaag
    19141 gagagccata gcccagtttt aacaatgcat cgcaggcatc aagataatca ttaaacgtat
    19201 ttttcttctt cagaaattta ccgtcttcgt accattgttg ccccagctca ccgccgccgc
    19261 gaacatggac aatggcgtag acaaagccac gatctaacaa actcaagcgg ctaaaactga
    19321 aatcggcatc aatacttgcg ccgtaagaac cgtagccata caccagcaac gggttgtgcc
    19381 ctttgcgaaa atgtttgcga tggtagacca acgaaaccgg aacttcgaca ccatcacggg
    19441 cgactatcca caggtgttca ctgcggtaat tcgccgcatc aaaaccagga acttctgttt
    19501 gttttaatac acgacgctca ccggtatcca tatccagttc aaacaaagtg tctggtgtgg
    19561 tcatggaaga ataaccataa cgcaatcgcg cggtttcagg ttctggattg taggcaatcc
    19621 aggtcacata ggctggatca tcaaaggcaa taccaatgac ttcccgggtc ttgcggttaa
    19681 tttggcgcaa actggttaac ccgcgctgac gctcttcaac caccagccag tcggtaaaca
    19741 gcgtaaaccc ttccagcatg atgttctcgc gtggcggaat taactcttcc cactgttgct
    19801 catcacgcat acgggtacgg tataagccaa agtttttgcc gtggcggttg gaacgcagat
    19861 aaaaccgatg ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc ggcagaaaaa
    19921 caaacggctc ggcatcggcc atttccgcgt ccagcaggcg aacttcactg gtggtggcgc
    19981 tggccaaatg aatgactaca tagtgcttcg aggtcgtttt atgcaggctg acgtaatagg
    20041 tatcgtcttt ttcttcgtaa atcagtttat cttgcgatgc tggcgtaccg atggcgtgac
    20101 gccagacctg ataaggcagc agcgtcaccg gatgcttgcg aacatagtag aaagtccagg
    20161 agtcatttgc ccagacaaag ctgggttcaa cgttatccag cagttccggg taccagttac
    20221 ctgtttccag attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa tcttctgcca
    20281 gcgccataat ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa aactcactat
    20341 gagctgcgcg cttattggca tcgagcaatg tttcccactc atcccactct tcactgaatg
    20401 ccgattgacg ctggtagata gcatattcac agcctggttc ataaatatgc cgatagcggt
    20461 agccattttt gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg atgatttcct
    20521 ttaagatgcg atcctgcaag gcttgttgtg aggccatcac ccgatgaccg taactatttt
    20581 cttgttgcag gtagtccagg acttctggct gagaacgcgt atcgtcccgc agccagtagt
    20641 aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg gcggcttttg
    20701 gtagcatgtt attgttcttt ctggttgaaa catcttataa gggtggcaaa actcaccggg
    20761 gatgcaagcg aaacagggga gtcattgctt agatgatgac aggtaatggc gcggatatcg
    20821 actgttatgc agacagagaa aatcagcctg ttcaaatggc tgtgcggttc tggatagtcc
    20881 gaaatagtca acttcaggct atccagagag cggaattatt ccgccaaagt gcgtttttgc
    20941 tgttcgagat cgcgttcaat gccttcacga acatcctggg ggattttcag cgcgtcaccc
    21001 agtgcattca ggtaactgcg ttccataaaa tggtcaatat caatagccgc gcaactcagg
    21061 aaatagattt ccagcgcctc ttcttcattg cggaccccgg tagccaggcg ttgtggatcc
    21121 agcggttgtt cgattgcctg ctcaatgagt acccgcccct gctcttccac gccggcttca
    21181 cgcaattgct ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc acttttagcg
    21241 gcaaagacca gcgcaaggat caaacgttcc gtacgctcat cgagcggcgt actttgcgcg
    21301 ccaaactgcg gttcgtcctg atgcgccgcg cgaattttat ctttgtattt attccacagc
    21361 accgtacccg ctaccgctcc gccgccaacc agtaacgcgt tggtgccata tttcgtaaga
    21421 agtttacgtg ctgatttatt tgcgaccagc agcccagcca gtccgcctaa tgcgcctggc
    21481 actaacagtt tgcccaatcc ctgatccgca gacgaggagg tagaagaact gctttgccca
    21541 agcagggatt gcagttgatt taaccagtta gccatatttg ctcctcaata accattttta
    21601 gtgcctgtag catagcggat gaggatgtca gaaaatgtat atcgagacaa aagatgcgca
    21661 aataagcggg gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg tatttactgt
    21721 atatcaggtg ataagggtat tttaggtgaa agtagtttgt cgttttgagt ggcattgttt
    21781 gatgctgatt gcccgatgcg acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt
    21841 ctggggcgga caaggcactc gcgccgcatc cgccaacggt caatcgaatt atcagaaccc
    21901 attaacgccg ctgatattcc gccattcccg ctttgcagtc cacgctgacc tggtaatgaa
    21961 tatcggcact tttaccacgc acggttagcg gtactgacca tttatcatct ttaccctgaa
    22021 tgtcctgcaa actgacccaa gccacgggat cggcctgacc gacaattttt tgatcatctg
    22081 cccaacgcgc cacgcgattt tgttgataat cacgttttac gctcgcggca attccggcag
    22141 catccagacc ttcacacttt gggaaagtga ccgacttgct ggtttcatta ttggcagcga
    22201 aaactgatgc gcaggcagaa accaacaaca gccctaaaaa cgcccctctt tttttcatgt
    22261 ttttctccat agcacaatga ttcaggagaa agcatggtac aaattgtcag gagcgcaagt
    22321 tgcttcaggc cgcgtgtgag gcatcttcct cttcggattc agcaccgacg acctgtgggg
    22381 acggtaattt gccggttttc agaatggtgc tcagcacatc tttttgttct gccagccata
    22441 gcgaaagggc ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc agccaggtgt
    22501 caagcagatc cgccgtatca agcattttgt cataagcatc ggcttccttt ttgctggtaa
    22561 acgacatttt ctcttcgccc tcacgaatga ctacgtattt aacttcaacc gccatttgca
    22621 gcctctcata ataactgtga ttttatacag tatatttctt ttcggttgag aaatcaacac
    22681 cagcaataaa gacacacgca aacgttttcg tttatactgc gcgcggaatt aatcagggga
    22741 tattcgttat gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat
    22801 taggctccgg tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg
    22861 tgattgccgt cgatcgctat gccgacgcac cagccatgca tgtcgcgcat cgctcccatg
    22921 tcattaatat gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt
    22981 atatcgtgcc ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaag
    23041 gactgaatgt tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc
    23101 gtcgcctggc ggcagaagag ctgcagctgc ccacttccac ttatcgtttt gccgatagcg
    23161 aaagcctttt ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta aaaccggtga
    23221 tgagctcttc cggcaagggg cagacgttta ttcgctctgc agagcaactt gctcaggcat
    23281 gggaatacgc tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg
    23341 ttaagtttga cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc gtccatttct
    23401 gtgcaccagt aggtcatcgc caggaagatg gcgactaccg tgaatcctgg caaccacagc
    23461 aaatgagccc gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac
    23521 ttggcggtta tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca
    23581 gtgaggtctc ccctcgtcca catgataccg ggatggtgac gttaatttct caagatctct
    23641 cagagtttgc cctgcatgta cgtgccttcc tcggacttcc ggttggcggg atccgtcagt
    23701 atggtcctgc agcttctgcc gttattctgc cacaactgac cagtcagaat gtcacgtttg
    23761 ataatgtgca gaatgccgta ggcgcagatt tgcagattcg tttatttggt aagccggaaa
    23821 ttgatggcag ccgtcgtctg ggggtggcac tggctactgc agagagagtt gttgacgcca
    23881 ttgaacgcgc gaagcacgcc gccggacagg taaaagtaca gggttaaacc cggcaaaaaa
    23941 acgctacaaa aatgcccgat cctcgatcgg gcattttgac ttttacagct tagcgccttc
    24001 tacagcttca cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc
    24061 cggaaccagc caggaaccac cgatgcacag cacgcttttc agcgccaggt agtcacggta
    24121 gttagcggga gaaataccac ccgtcgggca gaaacggacc tgggagaacg gacccgcgat
    24181 cgcctgcagg gctttcacgc cgccgttagc ttcagccggg aagaatttga actctttcaa
    24241 accgtagtcc atacccagca tcagttcgga aacagtgctg atccccggaa tcagaggaat
    24301 agtcccttcg gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg
    24361 tgcacccgct tcagtgactt ctgccagctg ctgtggattc agcaccgtac cggcacccac
    24421 aatcgcttca ggcacttctt tggcgatagc acggatagcg tcaactgcac actcggtacg
    24481 cagagtcact tccagaacgc gcaccccacc agcaaccaac gcttttgcca tcggcaccgc
    24541 gtgttccagt ttttttacca cgataaccgg tacaaccggg ccggtggtca ggattgattc
    24601 tgcacttgtt ttccagtttt tcatcagagt tttctctcgc ctgattacaa atttgtcgtc
    24661 ttaaaaagtg atacaggttg cgccctgttc ggcaccggac agtttttcac gcaaggcgct
    24721 gaataattca cgtcctgttc ccacgcgtga cgcgctcagg tcaggaatgt gtggttcgcg
    24781 agcagccagt tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc cattcacacg
    24841 aatgatgtcc ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt ctggtgttac
    24901 gtggatagct gacggcactt tacctgaagc gccggagagt cgtccatcgg taactaacgc
    24961 aattttgaaa caccggtcca ataatacacc aagtggcggc atgagtttat gtaattctgg
    25021 cattccgttc gcttttggcc cctgatgacg gacaacaacg acacaatcgc ggtccagcaa
    25081 acccgcttca aaggccggca taacgtcatg ctggctttca aaaacaaccg ctggcgcttc
    25141 aatcacctgg ttctcaaccg gcacggcaga ggttttcata accgcacggc ccaggttacc
    25201 gcttaacact tttgtcccac catgatgaga gaaaggttgt tcgaaggaag cgatcacatt
    25261 gctgtcgagt gatttttccg ccccttcccg ccagtccagt tcaccattat tcagccatgg
    25321 ttcaagggta taacgagaca gaccaaagcc tgccaccgta ttgacatctt catgcagcag
    25381 gcctgctttg agcagttcac gcaccagaac cggtacgcca cctgccgcct ggaagtggtt
    25441 aatatcggcc ggaccgttcg ggtagagacg tgccatcagc ggtacaacat cagaaaggtc
    25501 agagaagtca tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat
    25561 ggtgtggtta gtggaaccac cggtcgccag cagtgcaacg ataccgttca ccaccacttt
    25621 ctcatcgatc atcttaccga tcggcatcca ttcattacca ttaccggtca tgcgtgtaac
    25681 ctgacgcgca gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga
    25741 agagcctggc aactgcatcc ccataaactc caccaccatc tggttggtgt tggcagtacc
    25801 gtagaaagtg catgttcccg gcgcatggta agacgcggct tctgactcca gtaaggccat
    25861 gcggtccact ttaccttcgg cataaagctg gcgaatacgc actttttctt tatttggcaa
    25921 accgcttgcc atcggtccag acggcacaaa caccgcaggc aaatgaccaa acgacagggc
    25981 tgccatcgtc agacccggga caatcttgtc gcacaccccg aggaacagag caccatcaaa
    26041 catgttatgg gacagcccga ccgccgcaga catcgctatc acttcgcggc ttagcagcga
    26101 caattccatt ccatcctgcc cctgggtgac accatcacac atcgccggaa caccgcccgc
    26161 aacctgacca accgcattcg cttcatgcag ggctttacga atgatttctg gatagtgttc
    26221 ataaggctgg tgagcggaga gcatgtcgtt ataggaggtg atgatggcga tattgttacg
    26281 caacatgctt ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag
    26341 gttaccgcat gccaactgcg aacgatgaac ggtcgacgtt ttcgcttgtt ctatccgggc
    26401 gagataagca gagcgagtct cgcgcgaacg ttcaatgatt cgatttgtta cgcgtaacaa
    26461 ttgtggattc ataaaggctc ctgaaattga gttgtcagag caggatgatt cacaacgcgt
    26521 ttcattcaga ggatttatga gtgaaacgcc tgtaaccgga gctcataggg caaaaacgtt
    26581 tcagtcagtg taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa
    26641 aattgttcta caatctgtgc aagatcatgt taccggtaaa ataaccataa aggataagcg
    26701 cagatattac tcaaactcat tccaggaacg accatcacgg gtaatcatcg ccaccgaggc
    26761 aacgggtccc caggttccgg cctgatacgg tttcggcgca tcattgtcca tcgcccacgc
    26821 ctcagtaatg gagtctaccc atttccaggc ttcttccact tcatcgcgac gtacaaacag
    26881 tgcctgaata ccacgcatgg tttccagcag caaacgttca taggcatccg ccagatgcgt
    26941 ctgattaaag gtttctgaat agctcagatc cagcttggtg atttgcaggt tatgtttgtg
    27001 gtcaaggcca ggaactttat tcagtacctg gatatccacg ccttcatcag gttgcagacg
    27061 gatagtcagt ttattctgcg gcagatcctg ccacgactct ttaaacagat tcagttcagg
    27121 tgttttgaaa tagaccacga cttcagaaca tttggtcggc agacgtttac cagtacgcag
    27181 gtagaatggc acaccggccc agcgccagtt atcaatgtcg acgcggatcg ccacgaaggt
    27241 ttctgtattg ctgctcttgt tcgcgccctc ttcttccaga tatcccggca cttttttgcc
    27301 ctgggcgaag cccgcagtat attgcccgcg tacggttttt tcgcgtacgt tggagcggtc
    27361 gatgcggcgc agagacttca gtactttcac tttttcatcg cggatgctgt ctgcgctcag
    27421 gtcagacggc ggagacatcg caatcatgca aagaatttgc agcaggtggt tctggatcat
    27481 gtcgcgcatc tgaccggctt tatcaaaata gccccagcgc ccttcgatcc ccacttcttc
    27541 tgccacggta atctcaacat gatcaatggt gcgattgtcc cagttattca caaacaggga
    27601 gttagcaaaa cgcagcgcca acaggttcag caccgtttct ttaccaagat agtggtcgat
    27661 acggtaaacc tggcactcct cgaagtattc gccaacctga tcattgattt cctgcgaggt
    27721 cgccagcgac gtccccagcg gtttctccat gactacgcgt gccggtttag cattcagttt
    27781 tgcctcgcca agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt
    27841 aatggtgata cgattttttt gatccagcat cgcgccgaga cggctgaatg cagcagtgtc
    27901 attgacatcg agattacaaa aatccagacg tgcactcagg gtgtcccata aaccttcatc
    27961 aatggtttct ttcatgaaag tttcgagcgc ctcgcggaca actttggtat atgccgcttt
    28021 atcccagtca gcacgcccta cgccgataat ccgggtgtcc gggttgagct gaccggcttt
    28081 ttccagttga tacagggaag gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa
    28141 aatgaccagg tcacaggcct gggctgtttg cgttaccgcc atgtcattct ccttaagtta
    28201 actaacccgg tacttaagcc agggtatact tgtaattttc ttacggtgca ctgtactgct
    28261 tttacgagct tgcgaaaact gtaaacgctt atccacccgt gcgattacgg taaaagcgcg
    28321 taaagtgcgg caaaactgat aaaaaaatca tcgttttcca ttgggtaaaa atctgacact
    28381 gatcatgtta tgaaaaaaaa taacaacttt tttatctgct tttgtcatta acacagcaca
    28441 caggcgtaat atctgacaaa actgcatttc gatttctttc agtgcggaaa tcgtcattac
    28501 ccgtgagtct ctttacatca tgaatatgct ggaaaaaatc cagtctcagc tggaacattt
    28561 gagcaaatca gagcgcaaag ttgccgaggt cattctggct tcgcccgata acgcgatcca
    28621 ttcgagtatt gctgctctgg cgctggaagc caatgttagc gaaccgacgg tgaatcgttt
    28681 ctgtcgcagc atggacacgc gcggttttcc tgattttaaa cttcatctgg cacaaagtct
    28741 ggcgaatggc actccctatg ttaatcgcaa cgtcaatgaa gatgacagcg ttgaatcata
    28801 cacggggaaa atatttgagt ccgcaatggc aacgctcgat catgtccgtc attcactgga
    28861 taaatctgcc atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt
    28921 cggattaggc tcttcagccg ccgttgccca cgatgcgatg aataagttct ttcgttttaa
    28981 tgttccggtg gtgtactccg atgatatcgt gctgcaacgc atgagttgta tgaattgtag
    29041 cgacggagac gtggtggtgc tgatttctca caccggaaga acaaaaaatc tggtcgagct
    29101 ggcgcagctg gcacgcgaaa acgacgctat ggtgattgcc ctcacctctg cgggtacccc
    29161 gctcgcccgg gaagcaacgc tggcaattac cctcgacgta ccggaagata ctgacattta
    29221 tatgcccatg gtttctcgac ttgcacagct gaccgtgata gatgtgctgg cgacaggatt
    29281 tactttgcga cgcggtgcaa aattcagaga taacttgaag cgggtcaagg aagcgctgaa
    29341 ggaatcgcgt tttgataagc agttacttaa tttaagtgac gatcgctaaa agcgactgtc
    29401 actgttctaa tcttatacga catccgaatg agattaattt atcgccatcg cggcgttatt
    29461 tcattcggat ttcatgttca agcaacacct ggttgtttca gtcaacggag tattacatgt
    29521 ccagaaggct tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca gatcgcgata
    29581 ataatcttga aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg
    29641 gctcgcctga agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg
    29701 ggcgtcatgt ggctattctg ggtgacctcc aggggcccaa aatccgtgta tccaccttta
    29761 aagaaggcaa agttttcctc aatattgggg ataaattcct gctcgacgcc aacctgggta
    29821 aaggtgaagg cgacaaagaa aaagtcggta tcgactacaa aggcctgcct gctgacgtcg
    29881 tgcctggtga catcctgctg ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc
    29941 agggcatgaa agtgttcacc gaagtcaccg tcggtggtcc cctctccaac aataaaggta
    30001 tcaataaact tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca
    30061 ttaagactgc ggcgttgatt ggcgtagatt acctggctgt ctccttccca cgctgtggcg
    30121 aagatctgaa ctatgcccgt cgcctggcac gcgatgcagg atgtgatgcg aaaattgttg
    30181 ccaaggttga acgtgcggaa gccgtttgca gccaggatgc aatggatgac atcatcctcg
    30241 cctctgacgt ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc gacccggaac
    30301 tggtcggcat tcagaaagcg ttgatccgtc gtgcgcgtca gctaaaccga gcggtaatca
    30361 cggcgaccca gatgatggag tcaatgatta ctaacccgat gccgacgcgt gcagaagtca
    30421 tggacgtagc aaacgccgtt ctggatggta ctgacgctgt gatgctgtct gcagaaactg
    30481 ccgctgggca gtatccgtca gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg
    30541 aaaaaatccc gagcatcaac gtttctaaac accgtctgga cgttcagttc gacaatgtgg
    30601 aagaagctat tgccatgtcg gcaatgtacg cagctaacca cctgaagggc gttacggcga
    30661 tcattaccat gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc
    30721 taccaatttt cgccatgtcg cgccatgaac gtacgctgaa cctgactgct ctctatcgtg
    30781 gcgttacgcc ggtgcacttt gatagcgcta atgacggcgt agcagctgcc agcgaagcgg
    30841 ttaatctgct gcgcgataaa ggttacttga tgtctggtga cctggtgatt gtcacccaag
    30901 gcgacgtgat gagtaccgtg ggttctacta ataccacgcg tatattaacg gtagagtaag
    30961 tacgttgccg gatgcggcga aaacgccaca tccggcctac agttcaatga tagttcaaca
    31021 gatttcgaat attctgaagc aaacttgaac ttatcatcag gcgaaggcct ctcctcgcga
    31081 gaggcttttt tatttgatgg gataaagatc tttgcgctta tacggctgga tttcgcccgg
    31141 tttgcgagtt ttcagcaatt ttagtatcca ggtgtattgt tctggtcgcg gaccaacaaa
    31201 aatctcgact tcttcattca tccgccgcgc aatcgtatga tcatccgcct ctaacagatc
    31261 atccatcggt gggcgcacct gaatcgtcag acgatgcgtc ttgccatcat aaatcggaaa
    31321 cagcggtaca acgcgcgcac ggcacacttt catcaaacga ccaatcgcgg gcaacgtcgc
    31381 tttataggtg gcaaagaaat caacaaattc gctgtgctct gggccatgat cctgatcggg
    31441 taaataatat ccccagtagc cctgacgtac cgactggatg aatggtttaa taccatcatt
    31501 tctcgcatgc agacgaccgc caaagcgacg acgcaccgtg ttccagacat aatcaaaaac
    31561 cgggttgccc tgattatgga acatcgctgc cattttctgc ccttgcgagg ccatcagcat
    31621 ggcaggaata tcgacggccc aaccgtgcgg caccagaaag ataactttct cgttattacg
    31681 ccgcatctct tcgatgatct ccagcccttg ccagtcaacg cgcagctgaa ttttctccgg
    31741 cccgcgtatt gccaactcag ccatcattgc catcgcttgc ggcgcggtgg caaacatctc
    31801 atcaacaatc gcttcgcgtt cagcttcact acgttctgga aagcagagcg acagattgat
    31861 taacgcacga cggcgtgagc tttttcccag tcgtccggca aaacgtccca gccgtgccag
    31921 aatgggatca cggaactttg gcggcgttaa agcgataccc gccatcgctg ctacgcccag
    31981 ccatgctccc cagtagcgcg ggtggcgaaa ggatttatca aactcaggaa tgtattcgct
    32041 attatttttt ttcgtttcca tgcttttcca gtttcggata aggcaaaaat caatctggtg
    32101 atagtgtagc ggcgcaactt gccccgcacc aaataaaaaa gccggtactg actgcgtacc
    32161 ggctgcgaat ggatgttaat taatcaaacc gtagctgcgg cacaatctct ttggcctgcg
    32221 ccaggaattc gcgacgatcg gagccggtca gcccttcggt acgcggcagt tttgccgtca
    32281 gcgggtttac ggcctgctgg tttatccata cttcatagtg cagatgcggc ccggttgaac
    32341 gtccggtatt accggaaagc gcgatacggt cgccacgttt caccttctgt cccggtttca
    32401 ccagaatctt gcgcaagtgc atataacgcg tggtgtagct gcgaccatga cgaatagcca
    32461 cataataacc tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa
    32521 gcactggcgt accctgcggc atggcgaaat caacacctct gtgtggtgca acgcgaccgg
    32581 tcaccggatt agtacgacgc gggttaaagt tagacgagat acggaactgt ttcgccgtcg
    32641 ggaatcgcaa gaatcctttc gccagaccag taccgttacg atcgtagaat ttgccatcct
    32701 cagcgcggat tgcgtaataa tctttacctt ctgaacgcaa acgtacgccc agcagctggc
    32761 tttgctcacg tttaccatca agcatttctc gagacattaa taccgcaaat tcatcgcctt
    32821 ttttcagttt gcggaaatcc atttgccact gcatggcttt aatcactgcg ctcacttcgg
    32881 cgctggttaa accggcgttt ctggcgctag caacaaagct ccccccgacg gtacctttca
    32941 gcagattgtt aacccactct ccttgctgca tttcgctggt cattttaaaa ccgttagcag
    33001 cagtacggtc ataggttcgg gtttcacgac gagacacttc ccaggtgagg cgctgcagtt
    33061 cgccgtccgc ggttaatgtc caggagagtt gttgaccgat tttcaggtta cgcaattctt
    33121 tgtcggccgc agccagttgg gtgatatcac ccatatcaat accatactga ttgagaatgc
    33181 tgcttagcgt atcgccagtg gaaacaacat attcatgcac gcccgcttca ccggcaattt
    33241 tgtcatccag ttcgtcctgg ggaatggctt catcttcttg tgcagcttga tcaatcggtt
    33301 cactggcttc aggtaagagc gaacgaattt cgttctgttc cagctcaatg gttttgacaa
    33361 ttggcgtggc atcgcggtga taaacatagg gccgccagac agcgacggcc agagtaagaa
    33421 cggtgagcga ccccaacata acgcggtgtg gtcgcggtaa attattaaac gccagggcga
    33481 cagagcgggc tatctgttgc acgtaatcac ttcctcatta atctcctttc aggcagctcg
    33541 catactggtt ggctaattga ctcaggaatt ctgaatagct tgttttaccc agtttgatat
    33601 tcgtccccag gggatccaac gttcccatac gaacggatgt ccctcgtgcg acgctctcaa
    33661 cgaccgctgg cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact
    33721 gtgttcttat ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta acggtaaaat
    33781 gaccaagcgg tgtcagtccg aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga
    33841 aataaccttt ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg gttgaagcta
    33901 attgtgcctc aaaatccttc aggttggcgt caagtttggc tcgactttgc ggcataagtt
    33961 ccactaattt tccatggatt gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa
    34021 gatgcatgtt gaaatcgccg tgatggtgat cttcgtcact tttttccgcg tggtcgtgat
    34081 cctcatcatc gccgtgaata cttttcatca gtagcggttt cacatcttca agctgcgcaa
    34141 tcgttacctg cttcgcttct ggtaatttgc ttaccggttt ttgcataaac gcttccatct
    34201 ccgggccaac ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc gatggacgca
    34261 gtgaataatc atgttctgaa gcgccgtcag gaagtaaaac ctccgtttct gttaccccat
    34321 cagcaatggc agaagcgatg aacccaacgg gtttaagcga agcgacaacg gcggcatctg
    34381 cggcctgtgt tgcacctccc cagagagcgg cggataatgc tgcgaaaaga agcgtttttt
    34441 tatgtaacat aatgcgacca atcatcgtaa tgaatatgag aagtgtgata ttataacatt
    34501 tcatgactac tgcaagacta aaattaacat gacaagtctg gtttccctgg aaaatgtctc
    34561 ggtttctttt ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa
    34621 aattttgact ttacttgggc caaatggcgc aggtaagtcg acactggtac gggtagtgct
    34681 cgggctggta acacccgatg aaggggttat caagcgcaac ggaaaactgc gcatcggcta
    34741 tgtaccgcag aagctgtatc tcgacaccac gttgccactg accgtaaatc gttttttacg
    34801 cttacgccct ggtacacata aagaagatat tttgcctgca ctgaaacgtg tccaggccgg
    34861 gcacctgatt aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc gtgtactgtt
    34921 agcgcgagca ttgttaaatc gcccgcaatt attagtgctg gatgaaccca ctcaaggcgt
    34981 ggatgtaaat ggccaggtgg cgttatatga ccttattgac caactgcgtc gtgaactgga
    35041 ttgtggcgtt ttaatggttt ctcacgatct gcatctggtg atggcaaaaa ccgatgaagt
    35101 gctgtgcctg aatcaccaca tttgttgttc cggcacaccg gaagttgttt ccctgcatcc
    35161 ggagtttatt tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca
    35221 tcataatcat cgtcacgatt tacagggacg aattgttttg cgtcggggaa atgatcgctc
    35281 atgattgaat tattatttcc cggttggtta gccgggatca tgctcgcctg tgccgcgggt
    35341 ccgctgggtt cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac gctggctcat
    35401 gcctcattac ttggtgtcgc gtttggtttg ttgctggacg tgaatccatt ctatgcggtg
    35461 attgccgtta cgctgctgct ggcgggcggt ctggtatggc tggagaagcg tccacagctg
    35521 gcgatcgaca cgttattagg gattatggcg cacagtgccc tgtcgctggg cctggtggtc
    35581 gttagtctga tgtctaacat tcgtgttgat ttgatggctt acctgttcgg tgatttactg
    35641 gcagtgacgc cagaagatct catctctatt gcaattggcg tggtcatcgt ggtggctatt
    35701 ttgttctggc aatggcgcaa tttgctgtcg atgacgatta gcccggatct ggcgtttgtt
    35761 gatggtgtga aattacagcg agtgaaattg ttgttgatgc tggtgacggc attgacgatt
    35821 ggtgtagcga tgaaatttgt cggcgcgttg attattactt cgctgctgat tattcctgca
    35881 gctactgcac gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc tgttttggtg
    35941 gggatggtgg cagtaactgg cggtttaacc ttttccgcat tttacgatac gccggcgggt
    36001 ccgtcggtgg tgctatgtgc ggcactgtta tttattatca gtatgatgaa aaagcaggcc
    36061 agctaatcta tcgctgaaca catttgtcgg atgcggcgcg agcgcctttt ccgacctacg
    36121 gttcgctatc tctggtatat gcctgataag acgcgaatag cgtcgcatca ggcacactgc
    36181 caatgtcgga tgcggtgcga gcgaccaatc cgacttacgg catttctggc ggcgttatgc
    36241 caaagtgatt ccacgcccgc gtcgtcgcca tacgcccgcg cggtgtacgc tgcaaaaagc
    36301 cttgctgaat caaataaggt tccagcacat cctcaatggt ttcacgttct tcgccaatag
    36361 ccgccgccag gttatccaga cctaccggcc caccaaagaa cttatcgatt accgccagca
    36421 acaatttgcg gtccatataa tcgaaacctt cagcatcgac attcaacata tccagcgcct
    36481 gagcagcgat atctgccgag atggtgccat cgtgcttcac ttcggcgaaa tcacgcactc
    36541 gacgcagcag acggttggca atgcgcggcg taccgcgagc gcgacgagca acttccagcg
    36601 cgccgtcatc actcatctca agccccataa agcgtgcgct gcgactgacg atatattgca
    36661 gatccggcac ctgataaaac tccagacgtt gcacaatacc aaaacggtcg cgcaacggtg
    36721 atgtcagcga acctgcgcgc gtggttgcac caatcagggt aaacggcggc aaatcaattt
    36781 taatggagcg tgccgccgga ccttcaccaa tcatgatatc cagttggtag tcttccattg
    36841 ccgggtacag cacttcttca acaactggcg ataaacggtg gatctcatca ataaacagca
    36901 cgtcatgcgg ttcaaggtta gtgagcatcg cagccaaatc gcccgccttt tccagcaccg
    36961 gaccagaagt cgtgcgtaaa ttaacgccca tttcattggc gacaatgttg gcaagcgtag
    37021 ttttacccaa ccccggagga ccaaaaatca acaaatgatc gagggcatcg ccgcgcagtt
    37081 tcgctgcttt gatgaaaatc tccatctgtg aacgaacctg cggctgacca acatactctt
    37141 ccagtaattt agggcgaatg gcgcgatctg ctacatcttc cggcaaagtg gtaccggcag
    37201 aaatcagacg gtctgcttca atcatccttt acctcataac gcggcgcgta gggcttcgcg
    37261 aattaatgtt tcactgctgg cgtcagggcg agcgattttg ctcaccatgc ggcttgcttc
    37321 ttgtggttta tagcccagcg ccaccagcgc ggcaaccgct tcttgttcag catcgtcggt
    37381 cgccgggctg gcaggagacg tgagtaccag gtcggcggct ggcgtaaaga gatcgccatg
    37441 caaaccttta aatcggtctt tcatttcaac aatcaagcgt tcggcggttt ttttgccaat
    37501 acccggcagt ttcaccagtg cccccacttc ttcacgctca acggcattaa cgaactgctg
    37561 cgctgacatt ccggagagga tcgccagcgc caacttcggg ccgacgccgt tggttttgat
    37621 caactctttg aacaatgtgc gctcttgttt attgttaaaa ccgtacagca gttgcgcgtc
    37681 ttcacgcacc acaaagtggg tgaaaacgat cgcttcctga cccgcttcag ggagttcata
    37741 aaaacaggtc atcggcatat gcacttcata gcctacgccg cccacttcaa ttaacaccag
    37801 cgggggttgt ttttcaatga tgatgcctct gagtctgcct atcacatgac gctcctgcgt
    37861 aatgaatcaa agataatgct gtatgataaa aaaatgctgg atagatatcc agcgaaggat
    37921 gaagaaaact tgcgaggtgt ctcgatgaac tgaaaaatgg cacagtataa tttattctac
    37981 aggttatatt ggaagcaaat attttaatat tacatattca gtgaagaaat gcgtaataaa
    38041 aatatacatt gcgcctcctg aaaaaataaa ttttttatgc tattacgtat attcgtatct
    38101 atttcaatgg aatgacaacg tgaatattaa ttaccctgct gaatatgaaa ttggagatat
    38161 cgtctttaca tgtataggtg ctgccttatt tggtcaaata tcagctgcat caaattgctg
    38221 gagtaatcac gtcgggatca ttatcggtca taacggtgaa gacttcctgg ttgcagaaag
    38281 ccgggttccc ctttcaacca tcaccacgct atctcgtttt attaaacgtt cgtctaatca
    38341 acgctatgct ataaagcgat tagacgctgg gctgacagaa caacaaaaac aacgaattgt
    38401 tgaacaggtt ccctcccggc tacgcaaact ttaccacacc ggttttaaat acgaatcttc
    38461 gcgccagttc tgttcaaaat ttgtttttga tatttataaa gaagcgctat gtattccggt
    38521 gggtgaaata gagacgtttg gcgaattgtt aaatagcaat ccgaatgcaa aactcacttt
    38581 ctggaaattc tggttcctgg gttctattcc gtgggagcgt aaaaccgtca cgccagccag
    38641 tttgtggcat catccaggtt tggtgttgat tcatgcggag ggagtggaaa cgcctcagcc
    38701 ggaactgacc gaggcggtat aacttaacgc agtcgccctc tcgccaggtt cagccgcgat
    38761 tcgctcatct gcatcgcatt ctgactaacg tggcagtggg tgatggcaat cgccagcgca
    38821 tcggcggcat ccgcctgtgg attagcgggc agtttcagca aggtgcggac catatgctgc
    38881 acctggcttt tttcggcact accaatacct accaccgttt gctttacctg acgtgccgcg
    38941 tattcaaata ctggcaactc ctgattcacc gccgccacaa tcgccacgcc gcgcgcctgg
    39001 cccagtttca gggctgagtc agcgttcttt gccataaaga cttgttcaat ggcgaaataa
    39061 tcaggctgga actgggtgat gatttccgtc acgcccgcat agatgagttt cagacgagac
    39121 ggtaaatcat ccactttggt gcggatgcat ccgctaccca ggtaggacag ttgcctacct
    39181 acctggcgga tgacgccgta gccggtcacg cgcgaacccg gatcaatgcc gagaataata
    39241 gccatcacgc gtctccgttt tgctgtttag caggcctcat cagagagtcg ctgcgacctc
    39301 atcagagatt tcaccgttat ggtaaacttc ctgcacgtcg tcgcaatctt ccagcatatc
    39361 gatcagacgc atcagtttcg gtgcggtttc tgcatccata tcagctttgg tagacgggat
    39421 catggaaact tccgcgctgt ctgctttcag acctgccgct tccagtgcgt cgcgcacttt
    39481 acccatttct tcccatgcag tgtagacatc aatcgcgccg tcatcatagg tcacaacgtc
    39541 ttcagcacct gcttccaatg ctgcttccat gatggtgtct tcatcgcctt tctcgaagga
    39601 gatcacgcct tttttgctga acagataggc tacggaacca tcagtaccga ggttaccgcc
    39661 acatttgctg aatgcatgac gcacttcagc aacggtacgg ttgcggttgt cagacagaca
    39721 ttcaatcatg attgccgtgc cgccaggacc gtaaccttcg tagatgatgg tttccatgtt
    39781 cgcatcatca tcaccgccca caccacgtgc aattgcgcgg ttcagcgtgt cacgggtcat
    39841 gttgttagac agtgctttat caattgctgc acgcagacgc gggttagcgt ccggatcgcc
    39901 accgcccagc ttagccgcgg ttaccagctc acgaatgatt ttagtgaaga ttttaccgcg
    39961 cttagcatcc tgcgcagctt tacgatgtct ggtgttggcc catttactat gacctgccat
    40021 aaaaatatct ccagatagcc ctgcctgttc aggcagcgtt aattacaaac tgttcaatcg
    40081 cctgccggtt gctccaggac ttagtgagcg ccgccgcagc aggcgcatca agccacttgt
    40141 aagccagatg ttcagtgaaa acgatctgcc gctcgtgcgg aagcgcaaga cagaaccatg
    40201 attccgtatt acgcgtcacg cccggcgcat agcgatgacg taaatgtgaa aaaatttcaa
    40261 actctaccgt gcgctgacag tcaattaagg tcagttgttc agcgacaaca tcaatggtga
    40321 cctcttcctt tacttcgcgc atggcagctt gcggcgcggt ttcaccctct tccacgctgc
    40381 cggttaccga ctgccagaaa tcggggtcat cacgccgctg caacatcagc acccgtttcg
    40441 tatcttgtgc gtagatgacc actaagatcg aaacgggacg cttatacact ttatccttca
    40501 cgctgcctct tcgttgactg ccttcgctca tcccattcac atagttatct atgctcatgg
    40561 gagttcactc agttgccgcc tcgatgcaac gcgaatgatt tcgtgtattt gagtcatatc
    40621 agttattctc agccttcttc acaacctgaa tgctcagctc agccagtgca gtcgggttag
    40681 caaagctcgg tgcttcagtc atcagacacg ctgccgccgt ggttttcggg aaggcgataa
    40741 cgtcacggat attgtcggtg ccggtcagca gcatggtcag acggtcaaga ccgaatgcca
    40801 gacctgcgtg cggcggagta ccgtatttca gagcgtcgag aaggaagccg aatttctcgc
    40861 gctgttcctc ttcgttgata cccagaatac caaacaccgt ctgctgcata tcaccattat
    40921 ggatacgtac tgaaccaccg cccacttcgt aaccattgat gaccatatcg taagcgttcg
    40981 ccaccgcatt ttccggtgca gctttcagct ctgcagccgt catgtctttc ggtgaggtga
    41041 acggatggtg cattgccgtc aggccgcctt caccgtcgtc ttcaaacatc gggaagtcga
    41101 taacccacag cggtgcccat ttgctttcgt cggtcagacc aaggtcttta cccactttca
    41161 ggcgcagtgc gcccatcgcg tcggcaacaa ttttcttgtt gtcggcaccg aagaaaatca
    41221 tatcgccatc ttgcgcggca gtacgatcca ggatggcttc gatgatttct gcattaagga
    41281 acttcgctac cgggctgttg ataccttcca gacctttcgc gcgttcgtta actttgatgt
    41341 aagccagacc tttcgcaccg tagatcttaa cgaagttacc gtattcgtcg atctgcttac
    41401 gggtcagcga tgcgccgccc ggaacgcgca gagcggcaac acggcctttc ggatcgttcg
    41461 ccggacctgc aaatactgca aactcaacag atttcagcag atcagcaacg tcggtcagtt
    41521 ccatcgggtt acgcagatcc ggtttatcag aaccataacg gcgttcggct tccgcaaagg
    41581 tcattaccgg gaaatcgccc agatccacgc ccttcacttc cagccacaga tggcgcacca
    41641 gcgcttccat cacttcacgc acttgcggcg cggtcatgaa agaagtttcc acatcgatct
    41701 gagtaaattc aggctgacga tcagcgcgca ggtcttcgtc acggaagcat ttaacgatct
    41761 gatagtagcg gtcaaagccg gacatcatca gcagctgttt gaacaactgt ggggattgcg
    41821 gcagtgcgta gaatttacct ttgtgcacac gagaaggcac caggtagtca cgcgcgcctt
    41881 ccggcgtggc tttggtcagc atcggagttt cgatgtcgag gaagccgtgg tcatccataa
    41941 aacggcgcac caggctggtg attttagcgc gggttttcag gcgctgagcc atttccgggc
    42001 gacgcaggtc gaggtagcgg tatttcagac gcgcttcttc ggtgttgacg tggttagagt
    42061 caagcggcag aacatctgcg cggttgatga tagtcagcga ggacgccagc acttcgattt
    42121 cgccggtcgc catatcgcgg ttaatatttt tttcgtcacg cgcacgtacg gtgcccgtga
    42181 cctgaatgca gaactcatta cgcagttcag aggccagctt taacgcgtcc gcacgatccg
    42241 gatcgaaaaa tacctgcacg ataccttcgc ggtcgcgcat atcgatgaag atcaggctac
    42301 caagatcacg acgacggttg acccaaccac acagagtcac ctgctgcccc acgtgggaca
    42361 aacggagctg tccacaatat tctgtacgca tgagatatcc cttaacttag ctgccggcgg
    42421 atgccccctg ctgcgcaggt gaccaagtcg cagcgttagc tgtatgtcac aactgaatga
    42481 aaaaaggcgg ctattatact ggaaattctg ccgcaccgta agagcctggc ccgcgctgga
    42541 acgcctcgtt accactttat atcgggcctg aaatcagact ctacgccagt ttgctataaa
    42601 ggtgttgccc gaactcataa aaattaacaa aatttgtcgt tccgccatcg gctaatcgca
    42661 ttaaggtgtg aggcacgatt ttgttttttc aggagtcatt atgcttgaac ttaatgctaa
    42721 aaccactgcg ctggtggtga tcgatttaca agaaggcatc ttaccttttg ccggtggtcc
    42781 acatactgcc gatgaggtag ttaatcgcgc cgggaagctg gcggcgaaat ttcgcgccag
    42841 cggtcagccc gtgtttctgg tgcgcgttgg ctggtctgcc gattacgccg aagcattaaa
    42901 acagccggtt gatgccccct cccccgctaa agtgttgccc gaaaactggt ggcaacatcc
    42961 agctgcatta ggtgcaactg acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt
    43021 ttacggtacg gatctggagt tgcaattacg ccgccgaggt atcgatacaa tagtgttatg
    43081 tgggatctcg accaatatcg gtgttgaatc caccgcccgc aatgcctggg aacttggctt
    43141 taatctggtg attgccgaag acgcctgtag tgccgccagc gccgagcaac acaataacag
    43201 cattaatcat atctacccgc gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc
    43261 gttatgattt acatcggtct accgcaatgg tcgcatccta aatgggtgcg gttggggatc
    43321 accagtcttg aagagtatgc ccgccacttt aactgcgtgg agggcaacac cacgctttac
    43381 gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac agaccacaga tgacttccgc
    43441 ttctgtttta agtttccggc gaccatttcg catcaggcag cattacgaca ttgcgatgat
    43501 ttagtgactg aatttttgac ccgcatgtca ccgttggctc cgcgcattgg gcaatactgg
    43561 ctgcaactgc ctgccacatt cggcccacgg gagctgcctg cgctttggca ttttctcgat
    43621 tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc atccacagtt tttcgccaaa
    43681 ggggaagagg aacaaacgct taatcgcggt ttacatcagc gcggcgttaa tcgggtgatt
    43741 ttagacagcc gcccggttca tgcagcacgt ccacacagtg aagctattcg tgacgctcaa
    43801 cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg cgacaaatcc gctgatccgt
    43861 tttatcggta gtgatgatat gacgcaaaac cgggaattat ttcaggtctg gttacaaaaa
    43921 ttagcgcagt ggcatcagac cactacgcct tatctttttt tacatacgcc agatattgcc
    43981 caggccccgg aactggtaca taccctgtgg gaagacttac gtaaaacgct tccagagatc
    44041 ggagcagttc cggctattcc acagcaatct tctcttttct gaatttgcca cctatcatag
    44101 acaggtgcca tcggccattt taaagggagt ttgtatggta agcgcgctgt atgccgtttt
    44161 aagtgcgttg ttattaatga agttctcttt tgatgtcgtt cgcttgcgtt tgcagtaccg
    44221 cgttgcctat ggtgacggtg gttttagcga actgcaaagc gctattcgca ttcatggtaa
    44281 cgcggtggaa tatattccca tcgcgattgt gctgatgctg tttatggaaa tgaatggcgc
    44341 agaaacctgg atggtgcata tttgcggcat cgttttgctt gctggtcgtc tgatgcatta
    44401 ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc ggcatgagcg ccacctggtg
    44461 tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg ccctgggagt tggttttctc
    44521 cctgcgttag cgcacaatac gccactttct ttttcccgga tttttacgtt atgtctcacc
    44581 gcgacacgct attttctgcc cctatcgcca gactgggcga ctggaccttt gatgaacggg
    44641 tagctgaagt cttcccggat atgatccagc gttccgttcc cggctattcc aatattattt
    44701 ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg tacgcaggtt tacgatctgg
    44761 gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa cattcatcat gataattgca
    44821 aaattattgc catcgacaac tccccggcga tgattgaacg ctgccgtcgt catattgacg
    44881 cctataaagc ccctacgcca gtagacgtca ttgaaggtga tattcgcgat atcgccattg
    44941 aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt cctggaacct tccgagcgcc
    45001 aggcattact ggataaaatt tatcaaggcc tgaacccggg cggcgcgctg gtgctttcgg
    45061 aaaaattcag tttcgaagat gccaaagttg gtgaactgtt gttcaacatg caccacgact
    45121 ttaaacgtgc caacggttac agcgaactgg agatcagcca gaagcgcagc atgctggaaa
    45181 acgtgatgct gaccgattcc gtggaaaccc ataaagcacg cctgcataaa gccggttttg
    45241 agcatagcga gctgtggttc cagtgcttta actttggttc actggtggca ttaaaagcag
    45301 aggacgctgc atgatcgact ttggtaactt ttattctctg attgccaaaa atcatctttc
    45361 acactggctc gaaacgctgc ccgcgcagat tgctaactgg cagcgcgagc agcagcacgg
    45421 gttgtttaag cagtggtcaa atgcggtgga gtttctacct gaaattaaac cgtatcgtct
    45481 tgatttattg catagcgtta cggcagaaag cgaagagcca ctgagcgcag ggcaaattaa
    45541 acgcattgaa acgctgatgc gcaacctgat gccgtggcgc aaagggccgt tctcactgta
    45601 tggcgtcaac atcgataccg aatggcgttc cgactggaaa tgggatcgtg ttctgcccca
    45661 tctttctgat ttaaccgggc gcaccattct tgatgtcggc tgtggcagcg gttatcacat
    45721 gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg atcgacccta cgcaactgtt
    45781 tctgtgccag tttgaagcgg tgcgtaaact actgggtaat gatcagcgcg cgcatttgtt
    45841 accgttaggt attgaacaac ttccggcact gaaagccttt gataccgtct tttcgatggg
    45901 cgtgctttat catcgtcgtt caccgctgga gcatctctgg cagttgaaag atcaactggt
    45961 gaatgagggt gaactggtgc ttgaaacgct ggttattgat ggcgatgaga acacggtgct
    46021 ggtgccgggc gatcgttacg cgcaaatgcg taatgtctat ttcatccctt ccgcgctggc
    46081 gctgaaaaat tggctgaaga agtgtggttt tgtagatatc cgcattgcgg atgtgagcgt
    46141 taccactaca gaagagcagc gacgcactga atggatggtt accgagtctc tgtccgattt
    46201 cctcgaccca catgatccga gtaagacagt tgaaggttat cctgcgccta aacgcgcggt
    46261 gctgattgcg cgcaagccgt aaaggtctgg tattactgcc ggatgcggcg tgaacgcctt
    46321 acccagccta caaaaccttg ctaattcaat atattgcagg gactatgtag gcctgataag
    46381 catagcgcat caggcagctt tatgtttgca taacctcagc gcccgtttcc gggcgctttt
    46441 cacgtcttac gcctgaacag ctggctgatc aaacgccgtt aacttcggtg cgttacccgt
    46501 atatttttcg atatacacca gcgcagagtt accggcacag gcatttgcca gctgcgagct
    46561 ggggatatcc gccgttaaca cgttcgcact gccgttttta cacaagccat tttccagatc
    46621 tggccatgca ccttcatgca cgcataccac gccttttttg atcccgtcag tcaccacagc
    46681 gcctgtcagg atctgaccac gtttattcca gacacggacc agatcgccat tcgcaatacc
    46741 aaagcgagca gcatcttcgg tgtgaatagt gattggttca cgatcggcaa ccgcatattt
    46801 tttacgcagt tccgcatagt taagctgact gtgtaaacgg tgtgccggat gtgcggtcag
    46861 aagctgcaac tgcttctcgt cggcggtacc cttccactca tcaggcgcaa gccaggttgg
    46921 gtgtgccggg caatccttat agccaaattt ttccagcgtt ttggagtaaa tctcaatttt
    46981 gccgcttggc gtacccagcg catttttcac tggatcggcg cggaaatcac cataacgaac
    47041 atactgttcg ttcttctcgc tgcggcgcat ttcgatcagt ttattttgct gccagaaggc
    47101 attaaacatt ggcatagtga cgcgttgcgc acgggcacct ttctgagcgg catcatagaa
    47161 aaatttcagc cacgccattt catctttacc ttcggtatag atctcttttc caccaggttt
    47221 cagtaattca gcaagatcgg caaacacgtc aaaatcgtta cgcgcttcaa attgcggagc
    47281 gacagcctgc ttcatcggta caatatgctg gttgctgtaa tcaccggtca tcgtcaagtc
    47341 attgcgctca aacgatgtgg tgatcggtaa tacgatatct gcatgtttag cagctgcggt
    47401 ccagtagcat tcagaaacga cgatcatctc cggtttctgc catgctttga tcagacgatt
    47461 agtatcctgg tgatgggtaa agttaccgcc acccgcccac cagatcattt tgatattcgg
    47521 atacgtttgt tctttaccgt tatgctgata tttgccgcca ggattttcca gcgcatcgac
    47581 gatacgggca acaggaatcg ccgtcattcc gccatcatca gcggcctcgc tggcctgtcc
    47641 ggcaatcgcc gcagacattt ccggcagcac gccgccaaca cgcgtcgggt tgccaccatt
    47701 ggagtaatga taagagaaac caaaaccgcc gcccggtgta ccaatctgcc ctaacatcgc
    47761 cgccagcgtc accagcatcc agtgtttctg ttcaccatat tgctggcgct gaattcccca
    47821 gcctgccatc agcattgtgc gattcgccgc catcagttca gcgagtttga ctatctgcgc
    47881 ttccggtacg ccagtaattt ccgccgccca ggccgcgctc ttcggagtat tatcgctctt
    47941 acctgtcaga tattcttcaa actgcggata cccggtggtg tatttttcaa ggaacacttt
    48001 atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct aacatcagtg ccacgtcggt
    48061 gcccatattc ggcgcgatcc aggtggcatt atcgccaaag aattcgatgg tttcggagcg
    48121 gataggatca atggcaatca ctggtttgcc agattttttc agctgatgga agtattccag
    48181 cccttgctca tcggtactgc tccaggcaat ttttaaggta ttcagcgggt tcatccccca
    48241 cagcaccaca acctggctgt tttccagaat cagcggccag gaggtctgct gttcatacac
    48301 ctctacagaa ccgaccacat gcggcatgat cacctgtgcc gccccggttg aataatcgcc
    48361 gctatgcccg gaataaccgc ccgccaggtt catataacgt tgcagtaagg tttgcgcttt
    48421 atgcaacacg ccagaagagc gccagccgta tgatccggca aaaatagccg atggtccgtt
    48481 agctttgcga atgcgatcgt gttgctcatg aatcagtttt aatgcttgct cccagctcac
    48541 ctgtacatag gtatcttcgc cacgaccttt cgccggttgc agtggattat cgagatagct
    48601 ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt acctgatccg ccgccgtaga
    48661 ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc gaagaaacaa tcttgccgtc
    48721 cttcacttct acgttcatcg ctccccaacg tcccgcggtg aggattttac cgcccttctc
    48781 ttctgcccat gcgggtagcg gtgctgccga tgtcaccacc agcgctccag cggcaatacc
    48841 gctgtgttta ataaattcac gtcttgttaa tgtcataact tcctccctga tcaacgagga
    48901 tcactgtttc tcggtaatat ctttggcgtt gtactggaaa taccgcgtta aaatgtccag
    48961 ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg gcaatggacg gccacgcatt
    49021 gacggtgtaa tggtcggcgg caataggggc atgacaacca gcgcaatagg tatcgtcaag
    49081 tttttcagcg tattgccata gcggtttacg gtccgctaat gcgggatcgg taagcgcacc
    49141 ctgtaaagac gcctgacgcc attgattgcc gtattcgtca gcctgccatt ccccgtttac
    49201 cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc gccagccgtt gacctgccgc
    49261 caggtagagc gtgttttcac tgccctgcat ttgataaccc tgcaacagaa cgatcggctg
    49321 tttgccactg gcatcaacga cggtgagatc ggtaccagga ttcacggtag ccagctcgcc
    49381 tatgcgagaa gttttgaaag gataaatatg tgcgccatta gtcactgaag tagcggcttg
    49441 actttccagc tcatgcgccg cgttgtcatc catttttatt tctggcggaa aatgggcaat
    49501 gcctttatga caatcgatac aggtttcgcc gcccttttgt gctttgttat gcattttctg
    49561 cgcagattca ctttgcgagg caatatccat ggcatcaaaa gaatggcaac tacggcacgt
    49621 tgcagagtca gttgctttta attctttcca tactgtttcg gccatttcct ggcgatgagc
    49681 ttcgaactta tcgtcactgt ctattttgcc gctaacaaat tcatgataaa tatctttaga
    49741 tgcttttaat ttagcgaata aataatccat ccctgacttt ggaatatggc aatcggcaca
    49801 ttccgcacgt atccctttct ggttcgaaaa gtggacagtt ccctgatatt cctcaaaagg
    49861 tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc gacgttttat gtaaggcttt
    49921 ttgcgccagc aagaacccgc caccaccaac cacaaccgct atcagcaaaa acaataaccc
    49981 aatgcgtttt ttccctcgca tagattcctc taattaaatt ccagaacacc gaaatcattg
    50041 tataaataaa tatacaacga attg
//
LOCUS       DAPGRN010000021        49130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPGRN010000021 DAPGRN010000000
VERSION     DAPGRN010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.021"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1163
                     /locus_tag="RHW33_001346"
     CDS             <1..1163
                     /locus_tag="RHW33_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV1644869.1"
                     /translation="PIWNWKISQYNVSDIKFETIIKPQFADRTYFSNCSPVDPTSTRP
                     TYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDT
                     NVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLK
                     RIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMA
                     NHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHTIINGKKFKTSRAI
                     SSRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNAR
                     DSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1529..2596
                     /locus_tag="RHW33_001347"
     CDS             1529..2596
                     /locus_tag="RHW33_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1644870.1"
                     /translation="MDKSKRHLAWWVVGLLAVVAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2593..5328
                     /gene="rbbA"
                     /locus_tag="RHW33_001348"
     CDS             2593..5328
                     /gene="rbbA"
                     /locus_tag="RHW33_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV1644871.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5328..6452
                     /locus_tag="RHW33_001349"
     CDS             5328..6452
                     /locus_tag="RHW33_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1644872.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6525..6800
                     /locus_tag="RHW33_001350"
     CDS             6525..6800
                     /locus_tag="RHW33_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV1644873.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKIPVPQGIKWSDIESQVKALGGEI
                     KEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6797..7156
                     /locus_tag="RHW33_001351"
     CDS             6797..7156
                     /locus_tag="RHW33_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV1644874.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7206..8066)
                     /locus_tag="RHW33_001352"
     CDS             complement(7206..8066)
                     /locus_tag="RHW33_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HDV1644875.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTQEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(8098..8367)
                     /locus_tag="RHW33_001353"
     CDS             complement(8098..8367)
                     /locus_tag="RHW33_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV1644876.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            complement(8357..9865)
                     /locus_tag="RHW33_001354"
     CDS             complement(8357..9865)
                     /locus_tag="RHW33_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDV1644877.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWPLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDILIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            complement(9858..11216)
                     /locus_tag="RHW33_001355"
     CDS             complement(9858..11216)
                     /locus_tag="RHW33_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV1644878.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNLIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(11293..11574)
                     /locus_tag="RHW33_001356"
     CDS             complement(11293..11574)
                     /locus_tag="RHW33_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV1644879.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(11571..12044)
                     /locus_tag="RHW33_001357"
     CDS             complement(11571..12044)
                     /locus_tag="RHW33_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV1644880.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(12069..12815)
                     /locus_tag="RHW33_001358"
     CDS             complement(12069..12815)
                     /locus_tag="RHW33_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1644881.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(13014..13415)
                     /gene="nikR"
                     /locus_tag="RHW33_001359"
     CDS             complement(13014..13415)
                     /gene="nikR"
                     /locus_tag="RHW33_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV1644882.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(13421..14227)
                     /gene="nikE"
                     /locus_tag="RHW33_001360"
     CDS             complement(13421..14227)
                     /gene="nikE"
                     /locus_tag="RHW33_001360"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV1644883.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLRAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            complement(14224..14988)
                     /gene="nikD"
                     /locus_tag="RHW33_001361"
     CDS             complement(14224..14988)
                     /gene="nikD"
                     /locus_tag="RHW33_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV1644884.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAAALGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMQTHA
                     RETCQALGKSADDATLTAALDAVGLENAVRVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(14988..15821)
                     /gene="nikC"
                     /locus_tag="RHW33_001362"
     CDS             complement(14988..15821)
                     /gene="nikC"
                     /locus_tag="RHW33_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV1644885.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(15818..16762)
                     /gene="nikB"
                     /locus_tag="RHW33_001363"
     CDS             complement(15818..16762)
                     /gene="nikB"
                     /locus_tag="RHW33_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV1644886.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLLDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(16762..18336)
                     /gene="nikA"
                     /locus_tag="RHW33_001364"
     CDS             complement(16762..18336)
                     /gene="nikA"
                     /locus_tag="RHW33_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV1644887.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSIAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDEAQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(18447..19034)
                     /gene="acpT"
                     /locus_tag="RHW33_001365"
     CDS             complement(18447..19034)
                     /gene="acpT"
                     /locus_tag="RHW33_001365"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV1644888.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(19089..20138)
                     /locus_tag="RHW33_001366"
     CDS             complement(19089..20138)
                     /locus_tag="RHW33_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV1644889.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            20270..21487
                     /locus_tag="RHW33_001367"
     CDS             20270..21487
                     /locus_tag="RHW33_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1644890.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLALPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(21491..22048)
                     /gene="dcrB"
                     /locus_tag="RHW33_001368"
     CDS             complement(21491..22048)
                     /gene="dcrB"
                     /locus_tag="RHW33_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV1644891.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(22121..22786)
                     /locus_tag="RHW33_001369"
     CDS             complement(22121..22786)
                     /locus_tag="RHW33_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV1644892.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            23007..23252
                     /gene="tusA"
                     /locus_tag="RHW33_001370"
     CDS             23007..23252
                     /gene="tusA"
                     /locus_tag="RHW33_001370"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV1644893.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            23397..23705
                     /locus_tag="RHW33_001371"
     CDS             23397..23705
                     /locus_tag="RHW33_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000385055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1644894.1"
                     /translation="MDYLIPVCLGLLGLFAMFAPNWLMPVLIAVGGWLGVLACGWLWL
                     MIFLPLLNGVGLAYALWLMFKRPSFTSRLVATCCVISVILMLALYYTFLTMPPFPAQH
                     "
     gene            complement(23738..25936)
                     /gene="zntA"
                     /locus_tag="RHW33_001372"
     CDS             complement(23738..25936)
                     /gene="zntA"
                     /locus_tag="RHW33_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV1644895.1"
                     /translation="MSTPDNHGKKAPQFSAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEQQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPENATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(26010..26636)
                     /locus_tag="RHW33_001373"
     CDS             complement(26010..26636)
                     /locus_tag="RHW33_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV1644896.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            26777..27136
                     /locus_tag="RHW33_001374"
     CDS             26777..27136
                     /locus_tag="RHW33_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV1644897.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVIPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(27139..27408)
                     /locus_tag="RHW33_001375"
     CDS             complement(27139..27408)
                     /locus_tag="RHW33_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV1644898.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(27398..27994)
                     /gene="rsmD"
                     /locus_tag="RHW33_001376"
     CDS             complement(27398..27994)
                     /gene="rsmD"
                     /locus_tag="RHW33_001376"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV1644899.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            28144..29640
                     /gene="ftsY"
                     /locus_tag="RHW33_001377"
     CDS             28144..29640
                     /gene="ftsY"
                     /locus_tag="RHW33_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV1644900.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAKEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            29643..30311
                     /gene="ftsE"
                     /locus_tag="RHW33_001378"
     CDS             29643..30311
                     /gene="ftsE"
                     /locus_tag="RHW33_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV1644901.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            30304..31362
                     /gene="ftsX"
                     /locus_tag="RHW33_001379"
     CDS             30304..31362
                     /gene="ftsX"
                     /locus_tag="RHW33_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV1644902.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            31607..32461
                     /gene="rpoH"
                     /locus_tag="RHW33_001380"
     CDS             31607..32461
                     /gene="rpoH"
                     /locus_tag="RHW33_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV1644903.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            32732..33835
                     /gene="livJ"
                     /locus_tag="RHW33_001381"
     CDS             32732..33835
                     /gene="livJ"
                     /locus_tag="RHW33_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV1644904.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGVKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            33985..34251
                     /locus_tag="RHW33_001382"
     CDS             33985..34251
                     /locus_tag="RHW33_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV1644905.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAVKRLQGM"
     gene            34258..34785
                     /locus_tag="RHW33_001383"
     CDS             34258..34785
                     /locus_tag="RHW33_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1644906.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(34782..35165)
                     /gene="panM"
                     /locus_tag="RHW33_001384"
     CDS             complement(34782..35165)
                     /gene="panM"
                     /locus_tag="RHW33_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV1644907.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            35589..36698
                     /gene="livK"
                     /locus_tag="RHW33_001385"
     CDS             35589..36698
                     /gene="livK"
                     /locus_tag="RHW33_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV1644908.1"
                     /translation="MKRNAKTIIAGMIALAISHTALADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKDDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKVDKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            36746..37672
                     /gene="livH"
                     /locus_tag="RHW33_001386"
     CDS             36746..37672
                     /gene="livH"
                     /locus_tag="RHW33_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV1644909.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            37669..38946
                     /gene="livM"
                     /locus_tag="RHW33_001387"
     CDS             37669..38946
                     /gene="livM"
                     /locus_tag="RHW33_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV1644910.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            38943..39710
                     /gene="livG"
                     /locus_tag="RHW33_001388"
     CDS             38943..39710
                     /gene="livG"
                     /locus_tag="RHW33_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV1644911.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            39712..40425
                     /gene="livF"
                     /locus_tag="RHW33_001389"
     CDS             39712..40425
                     /gene="livF"
                     /locus_tag="RHW33_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV1644912.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            40544..41290
                     /locus_tag="RHW33_001390"
     CDS             40544..41290
                     /locus_tag="RHW33_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV1644913.1"
                     /translation="MHYAVVVMLELLCCIHAYRTGQERYWIFIIFCFPVIGCVAYFVI
                     VMLPETGADRHGHTLLMRLQDKLNPERHLRKLTEELAIAETNQNHYALANELARLGRY
                     HEAVPHYQQALSGIFAHEAAMMLSLAQAQFAIQEFAACQQTLEDVMRYNPDFQSADGH
                     LLFARTLAAQEKYADAESEFEVLISYYPGPQARIYYAEMLAKMSRLREANEQYVAVVD
                     TAKRSRPHYRKHHREWIKTANERLKQSVVQ"
     gene            41650..42966
                     /gene="ugpB"
                     /locus_tag="RHW33_001391"
     CDS             41650..42966
                     /gene="ugpB"
                     /locus_tag="RHW33_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV1644914.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            43064..43951
                     /gene="ugpA"
                     /locus_tag="RHW33_001392"
     CDS             43064..43951
                     /gene="ugpA"
                     /locus_tag="RHW33_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV1644915.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYIDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            43948..44793
                     /gene="ugpE"
                     /locus_tag="RHW33_001393"
     CDS             43948..44793
                     /gene="ugpE"
                     /locus_tag="RHW33_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV1644916.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            44795..45865
                     /gene="ugpC"
                     /locus_tag="RHW33_001394"
     CDS             44795..45865
                     /gene="ugpC"
                     /locus_tag="RHW33_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV1644917.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGCKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            45862..46605
                     /gene="ugpQ"
                     /locus_tag="RHW33_001395"
     CDS             45862..46605
                     /gene="ugpQ"
                     /locus_tag="RHW33_001395"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV1644918.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYGKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARELWAGMTPPLLSSFEIDALEAAQQAV
                     PELPRGLLLDEWRDDWRELTARLGCDSIHLNYKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(46592..47032)
                     /locus_tag="RHW33_001396"
     CDS             complement(46592..47032)
                     /locus_tag="RHW33_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV1644919.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPGTPQPDIPLKTIGP"
     gene            47152..48897
                     /gene="ggt"
                     /locus_tag="RHW33_001397"
     CDS             47152..48897
                     /gene="ggt"
                     /locus_tag="RHW33_001397"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV1644920.1"
                     /translation="MIKPTFLRRVAIAALFTGSCFSTVAAPPAPSPVSYGVEEDVFHP
                     VRAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLI
                     RSKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALD
                     KYGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLK
                     KGDKLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVER
                     TPISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYA
                     YADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQT
                     THYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGG
                     DANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAE
                     ATNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELY
                     GASDPRSVDDLTAGY"
BASE COUNT        11616 a        12543 c        13465 g        11506 t
ORIGIN      
        1 aacccatctg gaactggaaa atatcgcaat acaatgtatc tgatattaaa ttcgaaacaa
       61 ttataaaacc ccagttcgct gacagaacct atttctccaa ttgttcacct gttgatccca
      121 caagtaccag accaacctat tttggtgata ccgatggctc agttggtgca gttttgtacg
      181 cgctttttgc aacgggacat ttgggaatca tggctgaagg agaaaatttt cttagccagt
      241 tgcttaatat agaagatgaa gttctcaacg tattgctaag ggaaaacttt aatgagcaac
      301 ttgacactaa tgttaataca ataatcagta tcctgaacag aagagacatt attttagaaa
      361 gtcttcagcc ttatttagtt attaataaag acgcagtcac gccatgtacg tttctgggcg
      421 accaaaccgg cgatcgattt agcaatatct gtggcgatca atttatcatc gatttgttaa
      481 aacgcataat gagtatcaat gaaaatgtcc atgtattagc tggaaatcat gaaacgaatt
      541 gcaatggtaa ctatatgcaa aacttcacgc gcatgaaacc acttgatgag gatacctacg
      601 acgggataaa agattatcct gtatgtttct atgaccccaa atacaaaata atggcaaatc
      661 atcatgggat aacttttgat gatcagcgaa aacgttacat tataggaccg ataacagtct
      721 ctattgatga aatgaccaat gctctcgatc cggtcgaatt agcggaaatt attaataaaa
      781 agcatcatac tataattaat ggtaaaaagt ttaaaactag ccgggctatc tctagtcgat
      841 cctttaatcg ttatttttct gtttcgacag attacagacc taaacttgaa gcactattag
      901 catgctcaca aatgttaggt ataaatcaag ttgttgcaca taacggtaat ggtggaagag
      961 aacgtatagg tgaaacgggg acagttttag gtcttaacgc ccgtgacagt aaacatgctg
     1021 gaagaatgtt cagcatgcat aattgccaaa ttaaccctgg tgcagggcct gagataacca
     1081 ctccctggaa gtcttaccag catgaaaaaa acagaaatgg acttatgcct ttgattagaa
     1141 ggcgcacaat gttacagctc tgaaccatag acaacatgaa tattaatgtt actatctggt
     1201 tgagactgtt gataaaacgt aaaaaggaat gctctgttcc tgcaactgaa caggcgtgaa
     1261 acgctatcca acaggatgga taccgttttg tcagccagtt ttatagatca catttatgag
     1321 tatgcgtgtt tcatttacgg ccaggaatgc tgatgatgac aacattacta cccgttttca
     1381 ctaagccttc cccgttggcg ctcaatgctc tgcgcgctgg tcgtatttgt cgtttccttc
     1441 ttatcccgga tggaataatc cgctgaacgt cgttcggcgg cagttgtaac acgcgtcgtt
     1501 tgcttacagg gattatggcg ggtcgattat ggataagagt aagcgccatc tggcgtggtg
     1561 ggttgtcgga ttactggcgg tggttgctat cgtggcgtgg tggctgttgc gcccggcagg
     1621 tgtgccggaa ggctttgccg tcagcaatgg gcgcattgaa gcgacggaag tggatatcgc
     1681 cagcaaaatt gccgggcgta tcgataccat tctggtgaaa gaaggccagt ttgttcgcga
     1741 aggcgaagtg ctggcgaaga tggatactcg cgtgttgcag gagcagcgac tggaagccat
     1801 cgcgcaaatc aaagaggcac aaagcgccgt tgccgccgcg caggctttgc tggagcaacg
     1861 acaaagcgaa actcgtgccg cgcagtcgct ggttaatcaa cgccaggccg aactggactc
     1921 cgtggctaaa cgtcatacgc gttcccgctc gctcgcccaa cggggggcta tttctgcgca
     1981 acagctggat gacgatcgcg ccgccgctga gagcgccaga gcggcgctgg aatcagcaaa
     2041 agctcaggtt tcggcttcta aagccgctat agaagcggcc cgtaccaata tcattcaggc
     2101 ccaaactcgt gtcgaagcgg cacaagccac tgaacggcgc attgccgcag atatcgatga
     2161 cagcgaactg aaagccccgc gtgacggacg cgtgcagtat cgggttgccg agccaggcga
     2221 agtgctggcg gcaggcggtc gggtgctgaa tatggtcgat ctcagcgacg tctatatgac
     2281 cttcttcctg ccaaccgaac aggcgggcac gctgaaactg ggcggtgaag cccggctgat
     2341 cctcgatgcc gcgccagatc tgcgtattcc tgcaaccatc agttttgtcg ccagtgtcgc
     2401 ccagttcacg ccaaaaaccg tcgaaaccag cgatgaacgg ctgaaactga tgttccgcgt
     2461 caaagcgcgt atcccaccgg aattactcca gcagcatctg gaatatgtca aaacgggctt
     2521 gccgggcgta gcgtgggtgc gggtgaatga agaacttccg tggcctgacg atctcgtggt
     2581 gaggttgccg caatgacgca tctggaactg gttcccgtcc cgcctgtcgc gcaactggcg
     2641 ggcgtgagcc agcattatgg aaaaaccgtt gcgctgaaca atatcacact cgatattccg
     2701 gcccgctgta tggtcgggct gattggtccg gatggcgtcg ggaagtcgag tttgttgtcg
     2761 ttgatttccg gagcccgcgt cattgagcag ggcaatgtga tggtgctggg cggcgatatg
     2821 cgcgacccga agcatcgccg cgacgtctgc ccgcgcatcg cctggatgcc gcaggggctg
     2881 ggcaaaaacc tctaccacac cttgtcggtg tatgaaaacg tcgatttttt cgctcgcctg
     2941 ttcggtcacg acaaagcgga gcgggaagtc cgaattaatg agctgctgac cagcaccggg
     3001 ttagcaccgt ttcgcgatcg tccggcaggg aaactctccg gcgggatgaa gcaaaaactc
     3061 gggctgtgct gcgcgttaat ccacgacccg gaactgttga ttcttgatga gcctacgacg
     3121 ggggttgacc cgctctctcg cgcccagttc tgggatctga tcgacagtat tcgccaacgg
     3181 cagagcaata tgagcgttct ggtcgccacc gcctacatgg aagaggcgga acgcttcgac
     3241 tggttggtgg cgatgaatgc cggagaagtg ctggcaactg gcagcgccga agagctgcgc
     3301 cagcaaacgc aaagcgccac gctggaagaa gcatttataa atctgttacc gcaagcgcaa
     3361 cgccaggcgc atcaggcggt agtgatccca ccgtatcaac ctgaaaacgc agagattgcc
     3421 atcgaagcgc gcgatctgac catgcgtttt ggttccttcg ttgccgttga tcacgttaat
     3481 ttccgcattc cacgcgggga gatttttggt tttcttggtt cgaacggctg cggtaaatcc
     3541 accaccatga aaatgctcac cgggctgctg cccgccagcg aaggtgaggc gtggctgttc
     3601 gggcaaccgg ttgatccaaa agatatcgat acccgccgtc gggtgggcta tatgtcgcag
     3661 gcgttttcgc tctataacga actcaccgtg cggcaaaacc ttgagttaca tgcccgtttg
     3721 tttcacatcc cggaagcgga aattcccgcg cgagtggctg aaatgagcga gcgttttaaa
     3781 ctcaacgacg ttgaagatgt tctgccggag tcattgccgc tcggcattcg ccagcggctt
     3841 tcgctggcgg tggcggtgat tcatcgcccg gaaatgttaa tcctcgatga acctacttct
     3901 ggcgtcgatc cggtggcgag ggatatgttc tggcagttga tggtcgatct ctcgcgccag
     3961 gacaaagtga ctatcttcat ctccacccac tttatgaacg aagcggaacg ctgcgaccgt
     4021 atctcactga tgcacgccgg aaaagtgctc gccagcggta caccgcagga actggttgag
     4081 aaacgcggag ccgccagtct ggaagaggca tttatcgcct atttgcagga agcggcagga
     4141 cagagcaacg aagccgaagc gccgcccgtg atacacgaca ccacccacgc gccgcgccag
     4201 ggatttagcc tgcgccgtct gtttagctac agccgccgcg aagcactgga attgcgccgc
     4261 gatccggtac gttcgacgct ggcgctgatg ggaacagtga tcctgatgct gataatgggt
     4321 tacggcatca gtatggatgt ggaaaacctg cgctttgcgg tgcttgaccg cgaccagacc
     4381 gtcagtagcc aggcgtggac gctcaacctc tccggttccc gttactttat cgaacagccg
     4441 ccgcttacca gttatgacga gcttgatcgt cggatgcgtg cgggcgatat cacggtggca
     4501 attgagatcc cgcccaattt cgggcgtgat atcgcgcgtg gtacgcctgt ggaactcggc
     4561 gtctggatcg acggagcgat gcccagtcgt gccgaaacgg taaaaggtta cgtgcaggcc
     4621 atgcaccaga gctggctaca ggatgtggcg agccgacaat ccacacccgc cagccaaagc
     4681 gggctgatga atattgagac gcgttatcgc tataacccgg acgtaaaaag cctgccagcg
     4741 attgttccgg cggtgatccc gcttctgctg atgatgatcc cgtcaatgct aagcgccctt
     4801 agcgtggtgc gggaaaaaga gctagggtcg attatcaacc tttacgtgac ccccaccacc
     4861 cgtagcgagt ttttgcttgg caaacagctg ccgtacatcg cgctggggat gctgaacttt
     4921 ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg cagtttcctg
     4981 acgcttaccc tggcggcgct gctgtatatc atcatcgcca ccggaatggg gctgctgatc
     5041 tccaccttta tgaaaagcca gattgccgcc attttcggta cggcgattat cacgttgatc
     5101 ccggcgacac agttttccgg gatgatcgat ccggtagctt cgctggaagg gccgggacgt
     5161 tggatcggcg aggtttaccc gaccagtcat tttctgacta tcgcccgcgg gacgttctcg
     5221 aaagcgctgg atctgactga tttgtggcaa ctttttatcc cgttgctgat agccataccg
     5281 ctggtgatgg gcttaagcat cctgctgctg aaaaaacagg agggatgatg cgccatttac
     5341 gcaatatttt taatctgggt atcaaagagt tgcgcagtct actcggtgat aaagcgatgc
     5401 tgacattgat tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc gttacgccag
     5461 gatcgttgaa cctggcaccg atcgccattg ccgatatgga tcaatcgcag ttatcgaatc
     5521 ggatcgttaa cagcttctat cgcccgtggt ttttgccacc ggagatgatc accgccgatg
     5581 agatggatgc cggactggac gccgggcgct ataccttcgc gataaatatt ccgcctaatt
     5641 ttcagcgtga tgtcctcgct ggacgccagc cagatattca ggtgaacgtc gatgccacgc
     5701 gcatgagcca ggcatttacc ggcaacgggt atatccagaa tattatcaac ggtgaagtga
     5761 acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg gaaacccgga
     5821 tgcgctttaa cccgaacctt gatcccgcgt ggtttggcgg ggtgatggcg atcatcaaca
     5881 acattaccat gctggcgatt gtattgaccg gatcggcgct gatccgcgag cgtgaacacg
     5941 gcacagtgga acatttactg gtgatgccga taacgccgtt tgagatcatg atggctaagg
     6001 tctggtcgat ggggctggtg gtgctggtgg tatcgggatt atcgctggtg ctgatggtga
     6061 aaggcgtgct gggcgtaccg attgaaggct cgatcccgct gtttatgctg ggcgtggcgc
     6121 tcagtctgtt tgccaccacg tcaatcggca tttttatggg gacgatagcg cgttcaatgc
     6181 cgcaactggg gctgctggtg attctggtgc tgctgccgct gcaaatgctt tccggtggct
     6241 ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc atgccgacga
     6301 cacactttgt tagcctcgcg caggccattc tctaccgggg tgccggattc gaaatcgtct
     6361 ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt tttcaccatt gcgctgctgc
     6421 gattcaggaa gacgattggg acaatggcgt aaggaaggga aacttgactt catactgtga
     6481 tgtcatacta ttgatgtcat aaagtgaagt cataaggaca tgccatgaga accaaggtac
     6541 aggctttacg gaagaaacaa aaaaatacat tggatcaaat atttaaaatt cctgttcctc
     6601 aagggatcaa gtggtctgat atagagtcac aggttaaagc attaggcgga gaaattaagg
     6661 aaggaagagg ttcgcgttgt aagctcatac taaatatgag cgttgcgtgt ttccatcggc
     6721 ctcatccgtc gccagatacc gataaaggcg ctgtagaaag cgtgcgtgac tggttactaa
     6781 gtataggggt aaaaccatga tcaaactgaa gacgccaaac tctatggaga ttgccgggca
     6841 acctgctgtt atcacttacg tgccagagct gaatgcattt cgtggcaaat tcctgggctt
     6901 gtcaggttat tgtgattttg tctctgacag cattcagggg ctgcaaaaag aaggggaact
     6961 ttccctgcga gagtatcttg aggattgtaa agctgcgggt attgagccgt atgcgcgtac
     7021 agaaaagatt aagaccttta cgttacgcta tccagaatcg ctgagtgaac gccttaataa
     7081 tgcggctgcg cagcaacaag tctctgttaa tacctatatt attgaaacac tgaatgagcg
     7141 tttgaatcat ttataactta tagcggccca acaatttctg ttgcgccgct atatttttgt
     7201 ggctcttaag atgcctttct aaccaaccga tgcccctcat tcatcatcag gattttgcta
     7261 tacacatctt cctcgacggc gttttttgct gatgccatga ctcgtgccag attcgcctcg
     7321 ttgtgattat tcacatacgc tttgccgacg gcggtgataa acgctttgcg cagatcgctg
     7381 gcgatattta ctttagcgac gcgataatta acaaagctgc gtaatatctc cggggcgata
     7441 ccggagccgc cgtgaataac caacggcacc ggacagactt cggcaatgcg cttcagcagg
     7501 gggatatcga tacgcggtat atcgtccagg ccatgaacgt tgccaataga aactgccagc
     7561 atgtcgcaac cggtacgctc gacaaaggtc ttcacctttt ccggttccgt tttgcaatcg
     7621 gcttcgctga catgatcatc ttctttgccg agaatcgccc ccagctccgc ttcaaccggt
     7681 acaccatagg atttacagaa atcgacagct tcctgggtga aagcgatatt ctcttcaaaa
     7741 gagaatgcgg caccgtcaat catgaccgag gtaaagcccg cgcgcaccgc ctgtttcaca
     7801 tcctcctgag ttttcccgtg atccagatga agactgaccg gaacatccat tttatcggcg
     7861 tgacgtttca cgatttcata aatgtaatcg tatcccgaga gttgtgcatt ggttggcgca
     7921 atctgaatga agttgggtaa tcccgaacgc tcaatagcgt ttaaaattga aattgtcgtc
     7981 tccagattgg tggtattaaa tgcgcccgct aatacccgtt tttcctgaat gcggtcgagc
     8041 agtatccgtc cattaactaa tggcatattg aaaacctcat cttttaatat cagttaatta
     8101 ttcagcttca ccgaagcggt cgcgaaataa gtcggtgaga gtctgtatgg cctcgttttc
     8161 atcatcaccg tcgcagcgta tttccaccgt ttttccacct tttattccgg cgcgcataat
     8221 attcatcatg ctggtggcat taatatcatt accctcatag tgcagcgtca ctttacagct
     8281 atattttttg gcggctttcg ccagacaggc cgccggacgt gcatgcaggc cagtactgtt
     8341 tctgacctcc acggttttat tcaacatagt gttcctctcg atgatgtaat ggcatcacgc
     8401 cttcttgttg taatttcaac tgataccagc ttttccattt gtgctgataa cgctgtaatt
     8461 gatggtatct cgcccgattc ggtaacaaac gttgggctat agcgactggc ggcggtggaa
     8521 tatccggtgc ggcggttaat aatgctccga cagaaaccgc ctcgttaaat gcaattgctt
     8581 caaccggacg ctgtaaaata tccgccttta attgcagcca aagaggatta tgggttgccg
     8641 gaccgatcac ttttaacgcg ctggtgccag gcacgttaaa acaggcgaag cagttggcaa
     8701 attccatggt taatcccagt gagacgctgg caattaatat gtcaatattt aacgtatcgc
     8761 caagcccata aaataatcca cgggtatggc gatttttata gggcgaaccc gaaccgcgaa
     8821 gatgcggaat aaagacggga atgtcatcga gcggccagtt ccccgctagg taatccgcat
     8881 gtccgcgagt aagtgatgcg gcgatctcct catcggttag ccggaacgtg ttgcgcagcc
     8941 actccagcgc gaaaccgccc acgggcagcg acgcaaacag ggtgaactgg ttggcaagtg
     9001 agtaacagcc gtttgccagc ttgtcacgtt tagcctgttc atccagcgtc ggttgtgtat
     9061 ccagttgcag aatgccttcc gtggtccccg tcgagttaag gatatcgcct ggcatcatct
     9121 gcaacgctcg cgcacccacc atatggtcat gtccggccag cgtcacgctg accggttgcg
     9181 agaacccaag tgtcttgcaa agtgactcgc tgacccatcc ggcgtgctcg cctggctgca
     9241 ccaatggcgc aaatgccgta cagggaacgt gtaacaacgc cgccgctttc gctgaccatt
     9301 cgcctttgcg cacgtccaga cacagcgtgc ggctggctaa ggtgatatcc gtgcgctgtt
     9361 cgccagtcag ccgccagagc agcacttccg gcgcatgtag ccaacgcagg ccgcggcgat
     9421 tacgcagcgg gtaatgttcc agcaaccagc gcattttgaa ggcagagtaa ttgctgtgta
     9481 gtggtagacc gcagatgtca taaagtgccg cactgtctgc ctcgctaagc gttgccagat
     9541 actcttcacc gcgacggtca taccatgcca gcattggcgt caggatctcg ccatgctcgt
     9601 caaggaacac gcctgattcg ccaaaactgg caatgctgat ccgtctgact ggcagcggcg
     9661 aagcggcgtt cagttgcgct atcgcctggc ggacctcctg ccagagggcg tcgatatcga
     9721 aatcgacatc gccctgtggg gagatctgct ttgccgtcac gaatttatgc gcgcccaacg
     9781 tcgttgcgtc caggcaggaa aagcaggtga ctttgcaatt ggtggtgcca atatcgataa
     9841 cgatagctgc gctgttatca ggcataaacc gtgctccgcg tcattcggaa aatgccatac
     9901 agtaccaggg cgataaccgg caccagacca atggcgatga gattgccgtt gaagatttcc
     9961 agcagccaga agcggaacgg gttaccgcca tcaaggaaac tggaaacctg gccgctggag
    10021 ccttcggcaa atttgaagtt aacgtctttc gccatgccgg taataaacgg tgcgatttta
    10081 gtcgcaatcc acaggtcggc aataatcacc gggatcgccg ccagaacggt gcggaaaata
    10141 ttgccacggc tggctaaagc aatcatcgac gccattaccg ccaggttggc aaggtcgcca
    10201 agcggcagga cttcattacc tgggaggaca aacgcgataa agaccgagat tggcgttagc
    10261 agcagggcgg tggagataat cgccggatta cctacggcaa cggcgatatc cagtccgata
    10321 tagagatcgt cacggtcagg gtatttggca ttgagatact ttttaatggc ttcagacagc
    10381 ggtaataaac cttccatcag gatgcgaacc atgcgtggca ggatgaacat cacaccgcca
    10441 atgctgatgc caagcagcaa cacttttttg aaatcgtatc ctgcaatcac gccgagcagt
    10501 atgcccagaa tagtgccaac catcatcggt tcgccgaaga tgccaaactt tttctgtacg
    10561 gtttccgggt cgatatgaat acgattgagg ccggggatat ggtcgatgat tttgtcgacc
    10621 agcagaccga ccgggaagaa caccaccgaa gagagcgtcg gcaatgagat cccttccagt
    10681 ccaaagtatt tttgtaccag tggcgcagac cagtcggcga gtttaagtac taccaccgca
    10741 gcaatcgccg ccgccagcaa accaaagaac aggctgccgg tgctgtaata aaccaacgta
    10801 ccagcaaggg caaaatgcca gtagttccac atatctacgt caacggtacg ggttttattt
    10861 aacgccagca tcgccacgtt aatcagaatg gtcaggggaa taacaaacgg ggcaattggc
    10921 gaaccccatg taatagcgga aagcggcggc cagcctaaat ctaccaccgg taaattaatt
    10981 ccggtgcgtt caaccatcgc ctgggccgct ggcccgacat tgctggtcag aatatcgaaa
    11041 atggcataaa taccgacaaa accaatgccg actgttaatg acgaacgtaa ggctttagcg
    11101 ggttttactt taaaacagag agcaagaata aataacacca acggcaacat taccgtcggg
    11161 ccaaatccca atatatattg cacaatatta tagagggtat gcgcgatatc gttcataata
    11221 aacctctttt tcaggcgaga gcgcacctcg cgttagcgcc tgatggagtg ggttgaaatt
    11281 aattattatt aattaatttt taaggatcga aacaatttgc gccagtactt tctcttcgcc
    11341 aataccggta ataatcggca agccgttaac taccgggacg tcgagttcat aaggcacttt
    11401 agtcgttgag actaccagtg ctacgccatc agtatttcca ggtaaagagg caacgttggt
    11461 ttgggaataa ttgacgttaa gaccgtgctc tttacaatat tccatgactt tttctgttac
    11521 cgccgtggat gtggcaatac ccgtggcgca gacaaataaa atggtttgac tcatatttca
    11581 cctcgttctt tcattttgtt taagattact gtgtgtattt ctgttgtttc catcgcaagc
    11641 agggcttgca taaagggcgt atcctgaatc atttccataa tccagcccag ggcatttaat
    11701 tgtttattac tttcgcttaa ggcgagcaaa aaaatcaccc tgacaggtac ggggtcagga
    11761 tcgccgccca tatcggcaaa ctctaccggt tccggcagga tgccgacggc aattgcattg
    11821 tgcctgacat gtttatggtc cgtgtgtgga atagcgacgc acggtggttc ggtaggcagc
    11881 cccgttgggt acgtcgcctc acgtttcagt acgtgatcgg caaagtcagg gaccaccagc
    11941 tcggcggcaa ccaacggttg cgctaactga cggatgatct gttcaacgct taaccctgcc
    12001 ggaagtcgtt gaatataatt ttcctgaaac tgtatgcctt gcattttatt cctcttaaaa
    12061 aagtgtactt aactcacttc ccgccgttgc agcacggtgc caagataata tttatttcct
    12121 tttaaccaga cgttgccaaa ctcaacgggt aatcctcggg agaaaaagac taactgctcc
    12181 agatgcaaaa cgggtgcatc ttcactgata tctaaaaaat gaccgcgttc attaccaatt
    12241 aatcgcgcgg catagcgact ttccgagtaa cgaatttttc tttgcgacaa actttctatt
    12301 gttggaaata aattgtgttg attaaaatcg atttccgcga tgccggggca tagctcaata
    12361 ttgatacggt tctctatcag catcgctttt tcatcaccaa tcgaacgtaa acgttcaagg
    12421 taaagaatat cctgaccggg cgttattctt aatttctctg ctacataacg attagccggt
    12481 tcaatccgtg acgtaataac ttcagtggta aaatgtattt tctggctttc cagcgattcc
    12541 gcgaatgaca ataatccttc acctaacgga taggccacgt tctctttttt gacaaatgtt
    12601 cccttgccct ggacctgaat caacacgcct tcttttacca gttgggcgac cgctttttta
    12661 acggttcccc gactgacttc cagcatatcc ataatctggt attccgaagg gatgcgatcg
    12721 tcttccacca gatctccggt ataaatactt tctcttatcc actccaccac ttgttgatag
    12781 agcggtattt gtgacgactt acttaatgat ttcatggcgc gcacctgaac atttacagtt
    12841 gtctatggac aatcatggac aacttcaagc gtatagtagg tgaacaggcg aaaaagagga
    12901 tcgaagcgtc tcacattttg agggaccaaa tcgtgatcaa aaaaatgtga tggaagtcga
    12961 agtttggcag gaatttactg cgccgtgagg aaataacggc gcgttgggag gtgttaatct
    13021 tccttcggca ggcactgtaa atgcccgtgc cgcacgccgc gctgggcgat aacgtcatcg
    13081 gcaaaatgct gcacgtcacc catgtcacct ttcagcacgg cgatttccag acagtcgtcg
    13141 tggttgatat ggacatgcag cgtggcgacg gagaggtcgt ggtgatgatg ctgggtggaa
    13201 acaatacggc tggctaagtc gcgtttttcg tgttcataca catacgacag caccgcgaaa
    13261 ccttgtgtgc cgtgctgctg ggtagcttcc tgcgccaggg cactacgcag aatatcgcgg
    13321 atagcttcgg aacggttgtt ataaccacga cgctggctca ggctgtccag cgtctccagt
    13381 aaatcgtcat caagcgtgat ggtgactcgt tgcatttgcg ttaaaccttt tctgaggtgc
    13441 gacggcgcac ggggaatgcg ggtaataccg cgttttgtag cacacgtccg gcgtcagagg
    13501 aaaaggttaa tttatctccc accgcctggg tttcgacgat ttgtccgttg tccatcacca
    13561 tcactcgctg gcaaaagcgt tccaccaggc gtaagtcgtg ggtgatgaac aggcaggcag
    13621 tgccaaactg ttgttgtagc tttttcagca ggcgaatgac gcccgcctgt aacacgagat
    13681 caaggttaga aacggcttca tccagaatca gtagtttcgg ttcgaccgcc agtgcgcgag
    13741 ccaggcagac gcgctggagc tggccgccgc ttaactgcgg tgggcgcttg tcgagcacgc
    13801 tgtcgtcgag atcgaccgcg cgcaacattt cgctggcgcg cgccagttgt tcggattttt
    13861 tcagtgatag caggtggcgc atcggttcac gcaggatctc gcgcacggtt ttgcgcggat
    13921 tcacggcgct gatggagtcc tgaaatacca tctgaatatc gcggcggaac gctttacgct
    13981 gggcgcgatt gagctttgcc aacgattcgc cgcgccagct aatattccct tgtgagggag
    14041 actctaagcc taccagcagc cgcgcgaggg tgcttttccc gcagccgctg cgccccagca
    14101 gggcgacggt ttcgccgctt ttcagggtca gggaaacgtt attaagcacc gcctggtgct
    14161 gatgttttcc gctaaatcca ccgtgcgcat agtgatggga aaggccggaa acgttaagta
    14221 aagtcatgat gccagctcca taccgtagag ggcgagatga gcggaaacca ggctgcgcgt
    14281 caccgcatgt ttgggggcgt taaacagcgt ttcgacatcg ccctgttcga caatcttgcc
    14341 gtcagacatt accgccacgt catccgccag acgcgccacc acgcccatat catgagtgac
    14401 cagcagcatc cccggcgctt gtttttgcat aatgctttcc agcagatcga gaatgcgcgc
    14461 ctgtgctacc acgtcgaggt cggtggtcgg ttcatcggcg atgataaacg gcgactcgca
    14521 cagcaccgcc atcgcaatca tcatgcgctg caacatgccg ccgctcatct cgaacggata
    14581 cagctttagc acgcgcacgg cgttttccag ccccaccgcg tctaaagcgg cggtaagcgt
    14641 ggcgtcatct gcggatttcc ctaacgcctg gcaggtttcc cgcgcgtggg tctgcatggt
    14701 gcgtagcgga ttaaaggcgc tgcgcgggtt ctgcatgatg gtggcaattt tgatgccgcg
    14761 cagggcgcag ggagaaaccg gtttgccatc ggctaaaatt tcccccgccg tctggcgaac
    14821 gcctgcgggc agaattccca gcgccgcggc gcaggttaat gatttcccgc tgccgctacc
    14881 gccgactaac gccagcacgc gcccgcgttg cagggttaac gatacgccgt gcacaagcgg
    14941 ctgcgcggcc tgtagcgcga tattacgtag ttcaatctgt tgcggcatta gtgtgcgtgc
    15001 tccgtcacca gatgaggatc cagatgatcg cgcagtgcgt cacccaccag gttaaaggcc
    15061 atcacgctga taaacagcgc cagccccggc cagaacattt gcagcggctg ggtccagata
    15121 tactggcgcg cgtcgttaat catcacgccc cattcggcgg tcggcgcggt cacgccgagg
    15181 ccgaggaagg acatcccggc aacgtgcagc atcatatggc cgatatccag cgttgccagc
    15241 accagcagcg aggggatcac cgcacctgcc agatgatcaa caaacacccg cacatggcct
    15301 gcgccagaaa gccgtgatgc cagcacaaac tcgcgctggc gcagtgaaat caccaggctg
    15361 cgcaccatgc gtgcatacca cgcccagtgc gacagggcga tggcgataat tacgttggtc
    15421 agcccagtac cgagcacgcc aaccataaag aatgacagaa tcgaggtcgg gaaggtcata
    15481 aacatatcgg cgacgcgcat ggtagcctga tcaacgcgcc cgccaatcag cccggcgctg
    15541 ccgccaataa ccagccctaa tgtcagcacc agcagcaggc aggccattac cgaaccgagc
    15601 gacacgcggg tcgctgccat cagccgcgag aaaatatcgc gccctaagtg atcggtgccc
    15661 aaccagtgct gcgcatccgg cgaaagcagg cgcgacggca aatcaatcgc ctgggggtca
    15721 tacggcagcc accactggct ggtgagcgca atcagcgcca gtagggcgat aatgatcagc
    15781 gccaggcgta ccgaccagcg ggaagagagg aaaaagttca cgcgtgcgct ccttcatgac
    15841 gacgaatgcg cgggtccagc gcggcgttga gcaaatcgac aatcaaatta cagaccacaa
    15901 aaaccaccac catcatcagc gtaaagcact ggatcaccgg atagtcacgg ttaaaaatcg
    15961 ccgacaccgc atagcgcccg acgccaggcc aggcaaagat gttttcgata atcatcgtcc
    16021 cgccaatcag ttcgccaatg tgcatcccca cggcggtgat catcggcagc gaggcattgc
    16081 gcagaatatg acggcgttcg gtctgtttgt cattcaggcc gcgtagccgc gcccaggtga
    16141 cgtgacgctg accggcgacg tccaacatac tggcgcgcag taaacgcgcg ttaatcgcca
    16201 gcgacataaa ggcaatggaa accgcaggca aaatgatgtg ctgccagccg ccgtaaccca
    16261 tcgcgggtag ccattgcaga tacaccgaaa acgccatcac cagtaaaaac gccagccaga
    16321 agttaggcat cgacacgcca agaaacgcga tgaaacgtac ggcgaaatcc agcagacggt
    16381 cgcgatggcg cgccgcccag ataccgagcg gtacggaagt gagcagaatt aataccagcg
    16441 ccgcacctgc aagttccagc gtggcgggca ggaagttcag catatcgtcc agtaccgggc
    16501 gttggctggc gaatgagata ccaaagtcaa ggtgcagcgc cttccacaac caggtgccgt
    16561 actggacgta cagcggctga tccagtccca gcatggtgcg ggtagaggcc agcatctccg
    16621 gcgtcggcgg caggttagac agacgcaaat aatcgagcgc cgggtcgccg gtaccgaggc
    16681 gcagcatcag aaaaatgatc accgaggcgg cgagcaccat cgggatcagc agcagaaagc
    16741 ggcgtaatac gtaacgcaac attaaggttt caccggttta atctgttcga acggaatttc
    16801 ggtggcgatc ggcgcgtagg ggatgttacc cagctccggt tttgatacca ccatcattga
    16861 gatgtaactg ataggcagat aaaccgcctc gtcatgcaga cgggtcagaa tgtcgcgata
    16921 cagtgcctga cgttgtgctt cgtcatgggt cgccagcact tcgccgatct ctttatcaat
    16981 cagcggcttg tcggctaatc cttgctgtgc ctggaagtca gcgtgcgacg gtacgcgcat
    17041 tgaactcagg aaggcgtgtg gatcgtatgg cgcgccccag gtgcggtgga aaatcatgcc
    17101 aaaacgaccg tcgcgctgac gagcatagat actgctctct tcttcgccaa tcagcgagac
    17161 atctgcgcca atctggcgca tatcagcctg aatgatttcc gctatcgatt tgcttaacgc
    17221 atcggtgccg atgaacgaca gttcaatgcg cagcggctga ccatttttct cgcggatgtc
    17281 tttgcccgca ggcagcgtcc aaccggcttt ttccagcaac gctttcgctt tctgcggatc
    17341 gtactggcgc ggtttcaggc cgaggttggc gtagggcaca gaaggggcaa acagggtgtc
    17401 ggcgacctgc tgggtgccat acaacgcgtt atcaatcagc gattttttgt ttaccgcgta
    17461 attaagagct tcacgtactg ccagctcatt ggtgggggct ttggcggtat taagcgccag
    17521 catcacggtt tcgatcggct gtgacagttg ggtatggtaa gccgggttct ggctaaagcg
    17581 ggcgaaggta tcgagcggta ataacccttc gtttccgtac agcaggtcga tatcgccagt
    17641 ttcaaacgcc accgcgcggg tagtcgggtc cgggatgacg ttaaaagtga tcttcttaat
    17701 tgccggcttt tcgccccagt agttttcgtt acggacgaag acatcgtact gattcagttt
    17761 cgattcctgc aaaacccacg gtccggtgcc aatcggcgct ttaattccgt tcatggtttc
    17821 atggttttta aactgcgagg gggcgataaa gcggaaaggg cgcggcaggg ccagttcttg
    17881 caggaaagga tagtaggcgc ttttcagggt gatttgcagc tctgttttac tgagtgcttt
    17941 aacatcaaca atctggtttg ccagctccag ccaagcgtga cgttgacggt tatcgagcac
    18001 tgcgcggaag ttttctgccg ccgcctctgc atcaaacggt tcgccgttag agaatttcac
    18061 gtcatcacgc agggtgaagg tccaggtttt accatcttct gaatgagtcc agctttttgc
    18121 cagccacggg atcaccgaac cgtctgcctg atatttcacc aatggttcat aaaccatgct
    18181 ctgggcgaac atctggttag gcgtgtaaag gtgcgggttt agtggcccga cattcaccgg
    18241 ccaggcggtg gtgatttcat ctggtgcagc ggcatggacg ataaaagacg cacaagccag
    18301 cagcgcaaat agagtgcggc ggagtgtgga gagcatggta accccaatgg attaaaatag
    18361 atggcggaaa taagtatgac gattttaagt attcgtcata ctgattacct gttctggatc
    18421 aatagatggg cagtcgggtg ggcggatcag ttaactgaat cgatccattg cacactgtcg
    18481 gcggtgagcg taaagggggt gggggtgcag atcgcaaggc ttaaattttc tagctgacaa
    18541 tggctgactg acagcgagga gtgataggtg ctgtctacgc tgacgatttg ccaggcgctg
    18601 ccgccacgct gtttaacgat ggcttctttg cgcgtccaga tgcgccagaa catttccagt
    18661 tgctgatcag gatgcacggc gtccatttca gcgtgttccc cgaggctgaa cacggcgttc
    18721 gccagccagc gccagttggc gcgcgggcga atcacttcga tatcgcagcc gacttcgcct
    18781 tcatcactca acagcagggc gatatcgtca ccgctatggc ttaagttgaa ccatagcggc
    18841 gtttccggcg cgaatgcagg tttgccttgt tcgccataga tgatctccgg tagcggggaa
    18901 agcgtgtgcg aaagcaatgc acgccccgcc agccagcgtt cgcgtcgtgg accttgcgga
    18961 gcttgctcgc gtaaacccgg tggcagtgga gctgcgctta aggtcgaaac tttccccaga
    19021 actatccgat acatatcagg gccaacgtat aatggaaaat gaaagtgcgt atcgtatcac
    19081 ttgtcgcctc atcccggtaa ccgacttttc ggtctgcccg gccccagtaa aatcgccagt
    19141 ttgctgccgc ctttcgtcgt ttccatccag attttacaca cgctggttaa tggcaccgaa
    19201 agtagcatcc ctaccgggcc gagcagccat ccccaaatta acaatgaaag aaataccacc
    19261 atggtggaca tccccaggcg atggcccatc atccgtggtt ctaaaatatt gccgataacc
    19321 atatggacaa ctaaaaacaa tgcgccgacc agaatacatt cgtaaacacc attaaacagc
    19381 agcacctgaa tcattggcgg tacggcggaa attaccgcgc cgatattggg cacgtagttg
    19441 agcaaaaacg ccagtactgc ccacatcagc gcaaactgca cccccatcag ctccagcccc
    19501 agccagacga tgacacctgt ccataaactg agtagcgtct tcaatgcaag atagtgcgaa
    19561 acacctttta aggcgcggtg tagtccggcg atgtgaatct gtgggttatt cagcgcaaaa
    19621 cgcattttgt aagggacgtg gcgcacttca aatagcatga aaacaacggt catcaccagc
    19681 aaaagcacgc tcgccattgc cccggaaagc ccggtcatta gcgccgtggt gaaggtaacc
    19741 actttttccg agtccatccg ctgcagcatt cgctccggcg acatatgcaa attaagaaaa
    19801 ggcaacatct cctgcaattt aaaaagtttg cgcgtcagct ccttattaaa cttcggcagc
    19861 atagagataa attcgttaaa cgatgccgcc agtacgccaa ccagcgcggt tagtgcgatc
    19921 agcatcacca ccactacaat cgtaatggca acggggcgtt gtactccccg acgaataaac
    19981 caggtgacga gcgggttgag gacgatggca aaaaacagcg ccagtaacag ctgcacaatg
    20041 atatctgccg ctgcgtgaat gcccgcgagg atcactacca gcgaggccag cttgagcaga
    20101 atgtgcatgc ccgttttatc gggttgaggg gtttccattg gggcttcctt gtgacttttt
    20161 gtattaagtg tagcgggagt cacgccagca atattctgat tcttactgct gattttcacc
    20221 tcagcacatg gtaaaaatga aacactgttg taaaaatgtg gtgatcctca tgcccgaacc
    20281 cgtagccgaa cccgcgctaa acggattgcg cctgaatttg cgcattgtct ccattgtcat
    20341 gtttaacttc gccagctacc tcaccatcgg gttgccgctc gctgtattac cgggctatgt
    20401 ccatgatgtg atgggcttta gcgctttctg ggcaggattg gttatcagcc tgcaatattt
    20461 cgccaccttg ctgagccgtc ctcatgccgg acgttacgcc gatttgctgg gccccaaaaa
    20521 gattgtcgtc ttcggtttat gcggttgctt tttgagcggt ctgggatatc tgacggcagg
    20581 attaaccgcc agtctgcccg tcatcagcct gttattactt tgcctgggac gcgtgatcct
    20641 tgggattggg caaagttttg ccggaacggg atcgaccctg tggggcgttg gcgtggttgg
    20701 ctcgctgcat atcgggcggg tgatttcgtg gaacggcatt gtcacttatg gggcgatggc
    20761 gatgggtgcg ccgttaggcg tcgtgtttta tcactggggc ggcttgcagg cgttagcgtt
    20821 aatcattatg ggcgtggcgc tggtggccat tttgttggcg ctcccgcgtc cgacggtaaa
    20881 agccagtaaa ggcaaaccgc tgccgtttcg cgcggtgctt gggcgcgtct ggctgtacgg
    20941 tatggcgctg gcactggctt ccgccggatt tggcgttatc gccaccttta tcacgctgtt
    21001 ttatgacgct aaaggttggg acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt
    21061 tgtcggtacg cgtttgttat tccctaacgg cattaaccgt atcggcggct taaacgtagc
    21121 gatgatttgc tttagcgttg agattatcgg cctgctgctg gttggcgtgg cgactatgcc
    21181 gtggatggcg aaaatcggcg tcttactggc gggggcaggg ttttcgctgg tattcccggc
    21241 attgggtgta gtggcggtaa aagcggttcc gcagcaaaat cagggggcgg cgctggcaac
    21301 ttacaccgta tttatggatt tatcgcttgg cgtgaccgga ccactggctg ggctggtgat
    21361 gagttgggcg ggcgtcccgg tgatttatct ggcggcggcg ggactggtcg caatcgcgtt
    21421 attactgacg tggcgattaa aaaaacggcc tccggaacac gtccctgagg ccgcctcatc
    21481 atcttaaaac ttactgaata accagcgtat taatgatgtt ttctgcggtg gtctgcgctt
    21541 tttgctgatc gtcagctggc agagtaattt gcatggtcag cagttgatta cccacgttac
    21601 ccagaataac ggaagagtac gccgtctggc ctttcgcgga gataatactg tctaactgct
    21661 gcattttgtg acctttcagc tcaatggctt tgttggtcac tacttgcagc tgcggatcgc
    21721 ggctacgttg ctgatcttcc aggcgcttcg ccagcaccgc cagatcttct ttcggatcat
    21781 cgcccatgat gacgatgact gctttctgcc cggtggcgtc ggaccagaca tgcatgttat
    21841 tggcctgcgt tcccagctta ccgctctggt cggtcatatc cgctggcagc gagaaactta
    21901 acttgccatc aagcaggttg acgggattcc cggtagcgtt actttccgcg accgaaccct
    21961 gcgccgtagc gttagtgtct ttatcatcac aggccgcaag ccccataacc agcaggccaa
    22021 ttccgacata tttaaccaga ttgcgcattg acttcttcct ttcgataaac ggccataacg
    22081 gctcattcat ccatcttatc acaactctga taacgaacct ttaactcgcc tgcaaagcgt
    22141 tgatttcgga tttatctgcc agtcttttca acagcatatt gagtaatacg ccatacattg
    22201 gcaggaagaa aacgatactg attaacactt tgaaacagta atcgaccagc gcgatttcca
    22261 tccagtgttc agccataaag gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca
    22321 gcgtgtcgct gacgttaccg aacagtgtgg acgctgtcgg tgccagccac cagcggcgac
    22381 tctgacgcag gcggttaaaa acgtgcacgt cgaggatctg ccccagcgcg taggccatga
    22441 aactggcggt ggcgatacgg gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct
    22501 gccaggaacc catatagaat agcgacgaga tgacgtagga gatcagcaac gccgggatca
    22561 tcaccgcaaa gatgatacga cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg
    22621 tagcaaggaa aataaacggg aagctaaacg cgccccaggt ggtatggaaa ccaaaaatgg
    22681 agacgggaag ctgaaccaga tagttactgg aggtgatcac cagcagatga aataacgata
    22741 accagaacaa cgccttatag cgttgagttt gcgagaaaac gttcatattg tacctttttg
    22801 attaaccatt ggggtgaggg aacccaatac gtacgacacg tctgttacct taacataata
    22861 acgtgcttac ccttttttcg agccgccgca tgatactgct ttacgttgac aatgcaatgg
    22921 ttaattttca cgcaatcgtt aacctggttt gcttacggac tcacagggcg taaactagcg
    22981 ccgttttttt atgtgatgag aagaaaatga ccgatctctt ttccagccct gaccacacac
    23041 tcgacgcact tggcctgcgc tgcccggaac cagtaatgat ggtgcgcaaa acagtgcgca
    23101 atatgcagcc aggtgaaacg ttgctgatta ttgctgacga tccggcgacg acccgcgata
    23161 tccctgggtt ttgtaccttt atggaacacg aactggttgc caaagagacg gatggactgc
    23221 cttatcgtta tttgattcgt aaaggcggtt aaggtggatc acggactgtt accctaaccc
    23281 tctccccaga ggggcgaggg gaccgatcgc actcaatgtt gctgcttgaa accggtacgt
    23341 aggacggata aggcgttcac gccgcatccg gcaagcaagt gtaatggaat acacaaatgg
    23401 attatttaat tcctgtttgc ctgggattgc tgggattgtt cgcgatgttc gcccccaatt
    23461 ggctgatgcc tgttttaatc gccgtggggg gatggctggg ggtacttgcc tgtggttggc
    23521 tctggctaat gattttttta cctttactta acggcgtcgg gcttgcttat gccctgtggt
    23581 tgatgttcaa aaggccatca ttcacgtccc gcctggttgc tacatgttgt gttatctccg
    23641 tcattctgat gttagcgcta tattacactt tcctgaccat gccaccgttc ccggcccagc
    23701 actaaccctg atagccagag gctatcaggc aacaccttta tttcctgcgc aacaacctta
    23761 acgcattcgc cgttaccagc accgtcgcac ccgtatctgc cagcaccgcc agccatagtc
    23821 cggtcattcc taacagcgtg gtgacgagga agatcccttt cagccccagc gcaatagtga
    23881 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accagaccgc
    23941 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccgctgc
    24001 ccattgcaat cccgatggcg gcagctttca tcgctggcgc gtcgttaata ccgtcaccga
    24061 ccatcgccag cggcgcatgt tgattcagct cggtcaccgc tttgacttta tcttccggca
    24121 acaggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
    24181 tatcgccggt gaggatcacc cctttgacgc ccagcgcgtt cagttcactg atggcagttg
    24241 cagcatcggc gcgcagggta tcctgtaacg caatgacacc aagcacgtca tcgttacgta
    24301 ctaccagcac taccgtttgc ccggcgcttt ccagttcgtt aattaaacca gcaaatgcat
    24361 cagcgggatg tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa
    24421 tgccagaccc gaccagcgcc cgctgtgatt cggcggcggg aatggcgagt gcagcaacct
    24481 gtgcttctcg cacgatggcc tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
    24541 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    24601 gtttaccgac ggtcagcgta ccggttttat caaacgccac ctgggtaaca cgacctagct
    24661 gctccagcgc cgcaccgcct ttaattaacg ccccacgacg tgccgctgct gccagcccgg
    24721 aggtaatcgc cgcgggcgtg gagataacta acgcacacgg gcagccaatc agcagcagcg
    24781 tcagcccttt ataaatccac tcttgccagc tggcggcaaa cagcagcggc ggcaccagcg
    24841 ttaccagcag ggcgacggcc ataatcgctg gcgtgtaaat acggctgaaa cggtcgataa
    24901 accgctcaat gggagcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    24961 tggcgctggc tcccggttcg gacaatactt ccaacgtcac cagacggtct acgctggtgg
    25021 cgcctgcagg aactttatcg cccgtcgcac gctccaccgg aatggattcg ccggtcaggg
    25081 cgctttcatc aaaactggca aagggtgaga gcagtttacc gtcggcaggc aaacgcccac
    25141 ccgccgcaac ttcaatcaca tcgccagggc gcaggctgtt aattgccacc tcttcacgct
    25201 caccgttacg caggcgcgtg gcgttttctg gtttcagcgc cattaacgcg ctgacgccct
    25261 gacgcgcgcg gctggcggcc caaccttcca gtcgttcacc aatcaaaaac agcagcaaca
    25321 ccatcgcagc ttcagccgtt gcgccaataa acagcgcacc aatagcggct acgctcatta
    25381 acgtttcaat ggcgaagtaa ctgcctgatt tgatcagccg taatgcctga cgagcaatcg
    25441 ggtacagccc aaccagcgtg gtcgcgataa acgccagttg cccgaacgga tgattgaact
    25501 gctccagacc ccagctgatt gccatcatca cgattagcgt aatcagcggc agattctctt
    25561 tcaggcgcga ttcttgttgt tcttcggcgg cctgttcatc gcgtagggaa tagcccgctt
    25621 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatccacc accagttttt
    25681 cggtggcgaa caacacctgc acctgattca cgcctgcaag ctggcgcacg gcattttcta
    25741 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    25801 agacgttttc agagagagtt ggcgtgctgg aacatgcgcc gtcgcagcaa cagtcgttgg
    25861 cgttctgtac cgtggttagc ggtttgaacg cagaaaattg aggggctttc ttgccgtgat
    25921 tgtcaggagt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    25981 tctggagtcg actccagagt caagatttat cagagataca gcgagcggac gatgaggaag
    26041 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
    26101 tggctaccca gccagacaaa gttactgata aacagcaacg aagcgccgac aaacgcagag
    26161 agtgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    26221 gtcatgccga taaacgtgca gataggccaa cgatactctt ccagccgcgt ccagataatc
    26281 gccagtaaca gcgcgcccag aaccagcagt accaacggca gaggccagaa gaaagagagc
    26341 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    26401 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    26461 agccctgcca gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac
    26521 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
    26581 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    26641 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    26701 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt
    26761 ccgatgaggg ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat
    26821 catcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
    26881 ggctccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
    26941 tcgccgttcg cgccagcagg aagtgattcc ggcaggcacc agtatgcgct atgaggcaag
    27001 cttcaaaccg caaagcggag gaatggagca aacgtttcgc ctcgacgccc agcagtacca
    27061 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgttagctt
    27121 tgttggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    27181 aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt
    27241 cggtaaagtg gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag
    27301 cgccacgtta acgaagatat tcagcgggcg tgggaaggga tgcaccaggt tgaggattaa
    27361 aaatccccaa acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    27421 gtgcttcgcg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    27481 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    27541 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    27601 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt gcctttttgc gccaggaatg
    27661 acatcgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    27721 ttaactgctg agaaaccgcg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg
    27781 ataacgcttc cagtcccagc gcgccgcttc cggcgaagca atccagacat tgggcgtcaa
    27841 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    27901 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    27961 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    28021 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    28081 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
    28141 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    28201 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    28261 caggcgcaag agcctgtgaa ggcttcggaa caagccgttg aagagcagcc gcaggcgcat
    28321 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct
    28381 gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac aaccggaacc ggtcgtagaa
    28441 gaaacgccgg agccagtggc tatcgaacgt gaagagctgc cgttgccgga agacgtcaac
    28501 gccaaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    28561 gcggcggaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    28621 ctggctgccg aagcggcaga agaagcggtg atagtggttc ctccggcaga agaagagcag
    28681 ccggtggaag aaatcgctca ggagcaggaa aaaccaacca aagaaggttt cttcgcgcgc
    28741 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
    28801 cgcggtaaaa aaatcgacga tgatctgttt gaagagctgg aagaacagct gttgatcgcc
    28861 gatgtgggcg tggaaaccac acgtaaaata atcaccaatc tgacggaagg cgcatcccgc
    28921 aagcagcttc gtgatgccga ggccctttat ggcctgctga aagaagagat gggcgagatt
    28981 ctggcgaaag tcgatgagcc gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg
    29041 gtgggcgtca acggtgtggg taaaaccacg acgattggta agctggcgcg tcagtttgag
    29101 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc agcggttgaa
    29161 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
    29221 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
    29281 ctgattgccg ataccgccgg acgcctgcag aacaaatcgc acctgatgga agagttgaag
    29341 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
    29401 attgatgcca gcaccgggca gaacgcggta agccaggcca aactgttcca cgaagctgtt
    29461 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
    29521 tcggtggctg accagtttgg tattcctatc cgctatattg gtgtcggcga acgtattgag
    29581 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
    29641 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
    29701 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
    29761 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
    29821 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc
    29881 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
    29941 tggcgatccc gctgattatc gccggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
    30001 cggcgctgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttcgg
    30061 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaacaagccc gcggtactgc
    30121 tggcggacga accgactggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
    30181 ttgaagagtt taaccgcgta ggggtaaccg tattgatggc aacgcacgac atcaacctga
    30241 tctcgcggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    30301 gccatgaata agcgcgatgc aatcaatcat attcggcagt ttggcgggcg tctggatcgc
    30361 ttccgtaaat cggtcggcgg ctcaggcgac ggcggtcgta acgcgccgaa acgcgcgaaa
    30421 tcctcgccaa aaccggtaaa tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc
    30481 ttccacggcg cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg
    30541 gttatcgcca tctctctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaac
    30601 caggcggcga cgcagtatta tccgtcaccg caaatcactg tttatctgca aaaaacgctg
    30661 gacgatgacg ctgctgcggg cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa
    30721 gtgaattatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    30781 ggtgcgctgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    30841 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    30901 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    30961 ctggtcgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    31021 atcggtaaca gtgtacgcct gagtatcttt gctcgccgtg actccatcaa cgtgcagaaa
    31081 ctgattggtg cgacggatgg tttcatcctg cgtccgttcc tgtacggtgg cgcactgctg
    31141 ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa ttctggtgct gcgattgtca
    31201 tcggcggttg cggaagtggc acaggttttc ggaacgaatt ttgatatcaa tggtttatcg
    31261 ttcgatgaat gcctgctgtt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    31321 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    31381 tttccctgca atgggttccg tagcagggaa agagaccccg ttgtctcttc ccggtatttc
    31441 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    31501 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    31561 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    31621 gcaaagttta gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc
    31681 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    31741 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    31801 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    31861 cctgatgaaa gcagtgcgcc gttttaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    31921 cgttcactgg atcaaagcag agatccacga atacgtcctg cgtaactggc gtatcgtcaa
    31981 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    32041 tctgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    32101 caaagacgta cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    32161 ttccgacgac gattccgaca gccaaccgat ggcaccggtg ctctatctgc aggataaatc
    32221 atctaacttt gccgacggca ttgaagatga taactgggag gagcaggcag caaaccgcct
    32281 gaccgacgcg atgcagggtc tggacgaacg cagccaggac atcatccgtg cgcgctggct
    32341 ggacgaagac aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga
    32401 acgtgtacgc cagttggaaa agaacgcgat gaaaaaactg cgcgctgcta tagaagcgta
    32461 atttccgcta ttaagcagag aaccctggat gcgagtccgg ggtttttgtt ttttgggcct
    32521 ctacaataat caatttcccc tccggcaaaa cgccaatccc cacgcagatt gttaataaac
    32581 tgtcaaaata gctattccaa tatcataaaa atcgggtatg ttttagcaga gtatgctgct
    32641 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aacaacaaca tcacaacaca
    32701 cgtaataacc agaagaatgg ggattctcag gatgaacata aagggtaaag cgttactggc
    32761 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    32821 cgtgggcgca atgtccggtc cggtggcgca gtacggtgac caggagttta ccggcgcaga
    32881 gcaggcggtt gcggatatca acgctaaagg cggcgttaaa ggcaacaaac tgcaaatcgt
    32941 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    33001 cgacggcatt aaatatgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    33061 catctacgaa gacgaaggca ttttaatgat caccccagcg gcaaccgcgc cggagctgac
    33121 cgcccgtggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    33181 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    33241 acagcaatac ggcgaaggtc tggcgcgtgc ggtgcaggac ggcctgaaga aaggcaatgc
    33301 taacgtggtg ttctttgatg gcatcaccgc cggggaaaaa gatttctcaa cgctggtggc
    33361 gcgtctgaaa aaagagaata tcgacttcgt ttactacggc ggttatcacc cggaaatggg
    33421 gcaaatcctg cgtcaggcac gcgcggcagg gttgaaaact cagtttatgg ggccggaagg
    33481 cgtggctaac gtctcgctgt ctaacattgc gggcgagtca gcggaagggc tgctggtgac
    33541 caagccgaag aactacgatc aggttccggc aaacaaaccc attgttgatg cgatcaaagc
    33601 gaaaaaacag gacccaagcg gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt
    33661 gcaggcgggc ctgaatcagt ctgacgatcc ggctgagatc gccaaatacc tgaaagcgaa
    33721 ctccgtggat accgtaatgg gaccgctgac ctgggatgag aaaggcgatc tgaaaggctt
    33781 tgagttcggt gtgtttgact ggcacgccaa cggcacggcg accgatgcga agtaacggat
    33841 agataaatga aaatgcccga tgcgcttaac gtccatcggg tttttttatg tttgtcgaaa
    33901 tggttgtaga aagattaaac aatccaggat atgcttattg tacggtgatg tgccgtacat
    33961 attatacgga gcaggagagt tcacatgtct gctgttaaaa agcagcgtat cgatctgcgt
    34021 ttaactgacg atgacaaaag tatgattgag gaagcggcag cgatatctaa tcagtccgtc
    34081 agccaattta tgttgaacag cgcttcgcag cgggctgcgg aagtgattga acagcatcgg
    34141 cgggtgatcc tcaatgagga atcctggacg cgggtgatgg atgcgctgag taatccaccg
    34201 tcaccaggtg aaaagctaaa acgtgcggta aaacgtcttc agggaatgta ataatttatg
    34261 gatgatctga cgatagagat tctgaccgat gatgcagatt atgatctaca gcgattcgac
    34321 tgcggcgagg aagcgttaaa tctctttctg acgacacatc tcgttcgtca acatcgcaac
    34381 aaaattctgc gagcgtatat cctttgtcgc aacactccag aacgtcaggt gctgggatat
    34441 tacacattat gcggcagttg ttttgaacga gccgcattgc cctcgaaatc gaaacagaaa
    34501 aaaattccct acaaaaatat tcccagcgtt actcttgggc gtctggcaat tgatcgttca
    34561 ttacaggggc agggatgggg agcaacactg gttgctcatg ccatgaaagt cgtctggtca
    34621 gcctctttag cggtaggtat tcacggtctt tttgtcgagg cgctgaatga aaaagcccat
    34681 acgttttata aatcgctggg ctttatccct ttagtcgggg aaaacgaaaa tgcgttattt
    34741 ttcccaacca aatccattga actgcttttt acacagagcg attaacgctt ctcccagccg
    34801 ccctgctgtg ccgtaaatcc cagtgcctgc ataaacgccg tcatcacacc gcgatcttcc
    34861 acgcctgcat ccgccatcca ccagcatgaa acgccaggat tgttacgcaa aacctcttcc
    34921 agcagatatt gccccacacc gcgacgccgg gtgacttccc gcacgcgcag agaatccagt
    34981 gctccctcgg tgccgcttaa ggttacccgc acggcagcaa gcaggcgctc gttaaaacgc
    35041 gcggcgtaga tgcggtggtt atcgtcaacc tgtaaagagg aaggggaata ctccggccag
    35101 atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt ctaatcgaat gatggtcagc
    35161 ttcatgggta acccgtgtaa atcacaaaag tggaaccagt gtaacgaaat aatttaatcg
    35221 gaggctttct cttttttatt tcttttggca ggtgattaat tttttttcag caataattac
    35281 aaagttaaaa cattagagaa tgaaaaatac ccagcataat cccctgaatg gtagtgaatt
    35341 attccgccct ttgtgccgtt attttatgct gacaaaggca cttttttctg tttatctatc
    35401 aacatattca gaatattatc tgttcttaat cgactgaaaa atagggactt taatcgctat
    35461 tatcacaaaa tactgcgcta aacccttaat cagacaggca aaaacagtgc agtataaaaa
    35521 aagaacagtc tgatttgtta acacataaaa acaaagcaac acaacatcac gaatggggat
    35581 ttttgactat gaaacggaat gcgaaaacta tcatcgcagg gatgattgca ctggcaattt
    35641 cacacaccgc tttagccgac gatattaaag ttgccgttgt cggcgccatg tccggcccga
    35701 ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca ggcgattaaa gacattaatg
    35761 ccaaaggggg aattaaggac gacaagctgg ttggcgtgga atatgacgac gcctgcgacc
    35821 cgaaacaagc cgttgcggtc gccaacaaaa tcgttaatga cggcattaaa tacgttattg
    35881 gtcatctgtg ttcttcttct acccagcctg cgtcagatat ctatgaagac gaaggtattc
    35941 tgatgatctc gccgggagcg accaacccgg agctgaccca acgcggttat caacacatta
    36001 tgcgtactgc cgggctggac tcttcccagg ggccaacggc ggcaaaatac attcttgaga
    36061 cggtgaagcc ccagcgcatc gccatcattc acgacaaaca acagtatggc gaagggctgg
    36121 cgcgttcggt gcaggacggg ctgaaagcgg ctaacgccaa cgtcgtcttc ttcgacggta
    36181 ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg cctgaaaaaa gaaaacatcg
    36241 acttcgttta ctacggcggt tactacccgg aaatggggca gatgctgcgc caggcccgtt
    36301 ccgttggcct gaaaacccag tttatggggc cggaaggtgt gggtaatgcg tcgttgtcga
    36361 acattgccgg tgatgccgcc gaaggcatgt tggtcactat gccaaaacgc tatgaccagg
    36421 atccggcaaa ccagggcatc gttgatgcgc tgaaagtaga caagaaagat ccgtccgggc
    36481 cttatgtctg gatcacctac gcggcggtgc aatctctggc aactgccctt gagcgtaccg
    36541 gcagcgatga gccgctggcg ctggtgaaag atttaaaagc taacggtgca aacaccgtga
    36601 ttgggccgct gaactgggat gaaaaaggcg atcttaaggg atttgatttt ggtgtcttcc
    36661 agtggcacgc cgacggttca tccacggcag ccaagtgaaa atcccaccgc ccgtgaaatg
    36721 cgggcgggtt tagaaaggtt accttatgtc tgagcagttt ttatatttct tgcagcagat
    36781 gtttaacggc gtcacgctgg gcagtactta cgcgctgatt gccatcggct acaccatggt
    36841 ttacggcatt atcggcatga tcaacttcgc ccacggcgag gtttatatga tcggcagcta
    36901 tgtctccttt atgatcatcg ccgcgctgat gatgatgggc attgataccg gctggctgct
    36961 ggtggcggcg ggattcgttg gcgcaatcgt cattgccagc gcctacggct ggagtatcga
    37021 acgggtggct taccgcccgg tgcgtaactc taagcgcctg attgcgctca tctcggcaat
    37081 cggcatgtcc atcttcctgc aaaactacgt cagcctgacc gaaggttcgc gcgacgtggc
    37141 gctgcccagc ctgtttaacg gtcagtgggt ggtgggccat agcgaaaact tctctgcctc
    37201 tattaccacc atgcaggcgg ttatctggat tgttactttc ctcgccatgc tggcgctgac
    37261 aattttcatt cgctattccc gcatggggcg cgcctgtcgt gcctgcgcgg aagatctgaa
    37321 aatggcgagc ctgcttggca ttaacactga ccgggtgatt gcgctgacct ttgtgattgg
    37381 cgcggcgatg gcagcggtgg cgggcgtact gctcggtcag ttctacggcg tcattaaccc
    37441 ctatatcggc tttatggccg ggatgaaagc ctttaccgct gcggtgctcg gtgggattgg
    37501 cagcattccg ggagcgatga ttggcgggct gattctgggg attgcggagg cgctctcttc
    37561 tgcctatctg agtacggaat ataaagatgt ggtctcattc gccctgctga ttctggtgct
    37621 gctggtgatg ccgaccggta ttctgggtcg cccggaggta gagaaagtat gaaaccgatg
    37681 catattgcaa tggcgttgct ctctgccgcg atgttttttg tcctggcggg cgtctttatg
    37741 ggcgtacaac tggagctgga tggcaccaaa ctggtggtcg acacggcttc agacatccgt
    37801 tggcagtggg tgtttatcgg cacggcggtg gtctttttct tccagctttt gcgaccggct
    37861 ttccagaaag ggttgaaaag cgtttccgga ccgaagttta ttctgcccgc cattgatggc
    37921 tccacggtga agcaaaagct gtttctcgtg gcgctgctgg tgcttgcggt ggcgtggccg
    37981 tttatggtct cacgcgggac ggtggatatc gccaccctga ccatgatcta cattatcctc
    38041 ggactcgggc tgaacgtggt tgttggtctt tctggtctgc tggtactggg gtacggcggc
    38101 ttttacgcca tcggcgctta cacttttgcg ctgctcaatc actattacgg tttgggcttc
    38161 tggacctgtc tgccgattgc cgggttaatg gcggcggcgg cgggcttcct gctcggcttt
    38221 ccggtgctgc gtctgcgcgg tgactatctg gcgatcgtta ccctcggttt cggcgaaatt
    38281 gtgcgcatat tgctgctcaa taacaccgaa attaccggcg gcccgaacgg tatcagtcag
    38341 atcccgaaac cgacactctt cggactcgag ttcagccgta ccgctcgtga aggcggctgg
    38401 gacacgttca gtaatttctt tggcctgaaa tacgatccct ccgatcgtgt catcttcctc
    38461 tacctggtgg cgttgctgct ggtggtgcta agcctgtttg tcattaaccg cctgctgcgg
    38521 atgccgctgg ggcgtgcgtg ggaagcgttg cgtgaagatg aaatcgcctg ccgttcgctg
    38581 ggcttaagcc cgcgtcgtat caagctgact gcctttacca tcagtgccgc gtttgccggt
    38641 tttgccggaa cgctgtttgc tgcgcgccag ggctttgtca gcccggaatc cttcaccttt
    38701 gctgaatcag cctttgtact ggcgatagtg gtgctcggcg gtatgggctc gcaatttgca
    38761 gtgattctgg cggcaatttt gctggtggtg tcgcgcgaat tgatgcgtga tttcaacgaa
    38821 tacagcatgt taatgctcgg tggtttgatg gtgctgatga tgatctggcg tccgcagggc
    38881 ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg gcgcagcgaa aggagagcag
    38941 gcatgagtca gccattatta tctgttaacg gcctgatgat gcgcttcggc ggcctgctgg
    39001 cggtgaacaa cgtcaatctt gaactgtacc cgcaggagat cgtctcgtta atcggcccta
    39061 acggtgccgg aaaaaccacg gtcttcaact gcctgaccgg tttctacaaa cccaccggcg
    39121 gcaccatttt actgcgcgat cagcatctgg aaggtttgcc ggggcagcaa atcgcccgca
    39181 tgggcgtggt gcgtaccttc cagcatgtgc gtctgttccg tgaaatgacg gtaattgaaa
    39241 acctgctggt ggcgcagcat cagcaactga aaaccgggct gttctctggc ctgttgaaaa
    39301 cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg cgccgccacc tggcttgagc
    39361 gtattggttt gctggaacac gccaaccgtc aggcgagtaa cctggcctat ggtgaccagc
    39421 gccgtctgga gatcgcccgc tgtatggtga cgcagccgga gattttaatg ctcgacgaac
    39481 cggcagcagg tcttaacccg aaagagacga aagaactgga tgagctgatt gccgaactgc
    39541 gtaaccatca caacaccact atcttgttga ttgaacacga tatgaagctg gtgatgggaa
    39601 tttcagaccg aatttacgtg gtcaatcagg ggacgccgct ggcaaacggt acgccggagc
    39661 aaatccgtaa taacccggac gtgatccgtg cctatttagg tgaggcataa gatggaaaaa
    39721 gtcatgttgt cctttgacaa agtcagcgcc cactacggca aaattcaggc gctgcatgag
    39781 gtgagcctgc atatcaatca gggcgagatt gtcaccctta ttggcgcgaa cggggcgggg
    39841 aaaaccacct tgcttggcac attatgcggc gatccacgcg ccaccagcgg gcgaattgtg
    39901 tttgatgata aagacattac cgactggcag acggcgaaaa tcatgcgcga agcggtagcg
    39961 attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg tggaagagaa cctggcgatg
    40021 ggcggcttct ttgccgaacg cgaccagttc caggagcgca taaagtgggt gtatgaactg
    40081 ttcccgcgtc tgcatgagcg ccgtgttcag cgggcaggca ccatgtccgg tggtgaacag
    40141 cagatgctgg cgattggtcg cgcgctgatg agcaacccgc gtttgttgtt gcttgatgag
    40201 ccatcgctcg gtcttgcgcc gattatcatc cagcaaattt tcgacaccat cgagcagctg
    40261 cgcgagcagg ggatgactat cttcctcgtc gagcagaacg ccaaccaggc gctgaagctg
    40321 gcggatcgcg gctacgtgct ggaaaacggc catgttgtgc tctccgatac cggcgatgcg
    40381 ctgctggcga acgaagcagt gagaagtgcg tatttaggcg ggtaaattat cgaatacccg
    40441 gcagccacgt tctgccgggc tcttcgtccg tatacagact tgtttcaact gaccgggagg
    40501 attaaaacca gaccacttat ttgccacgga aaggttccct cccatgcatt atgccgttgt
    40561 tgtcatgctt gaactgcttt gctgtattca cgcttaccgt accggacaag agcggtactg
    40621 gatttttatc attttctgtt ttccggtgat tggctgcgtg gcgtatttcg tcatagtgat
    40681 gctgcctgaa acgggcgctg accgtcacgg gcatactttg ctgatgcgcc tgcaggacaa
    40741 actcaaccct gagcgccacc tgcgcaaatt aaccgaagaa ctggcgattg ccgagacgaa
    40801 tcaaaaccat tatgcgttgg ctaatgaact ggcgcgtctg gggcgttatc acgaagctgt
    40861 tccccattat cagcaggcgt taagcgggat attcgcccat gaagcggcaa tgatgctaag
    40921 cctggcgcag gcacagtttg ctattcagga gttcgccgcc tgtcagcaga cgctggaaga
    40981 tgtgatgcgt tataaccctg attttcagtc ggcagacggg catttgttat ttgccagaac
    41041 gttagccgca caagaaaaat acgctgatgc ggaaagcgag tttgaagtat tgatttctta
    41101 ttaccccggc ccgcaggcgc gtatttacta tgcggaaatg ttggcaaaaa tgagtcggtt
    41161 acgtgaagcc aacgagcagt atgttgctgt agttgatacg gctaaacgct caagacctca
    41221 ttatagaaaa caccaccggg aatggattaa gaccgccaat gaacggctga agcagagtgt
    41281 ggtgcaatga aaatcttctt tgccgttaga ccagtgtcgc ggcaatcttt tgttaaaatt
    41341 gaaaaatgta gctctcatcg ggcagcaaaa ctggacgcag aatttgctgc catttttgct
    41401 cttcatggta aggagttctg gcgattagct ggccgttaat cgcctctttt cagtgcattc
    41461 tcgggatatc cgtgccccat ctcccatttt ccctcacccg taccgtcacc gccttgtcat
    41521 ctttctgaca ccttactatc ttacaaatgt aacaaaaaag ttatttttct gtaattcgag
    41581 catgtcatgt taccccgcga gcataaaacg cgtgaattcg cgcattcggt acaacaagag
    41641 agataaacga tgaaaccgtt acattataca gcttcagcac tggcgctcgg actggcgtta
    41701 atggggaatg cacaggcagt gacgaccatt ccgttctggc attctatgga aggggaactg
    41761 ggtaaagagg tggattctct ggcccaacgt tttaacgccg aaaacccgga ttacaaaatt
    41821 gtaccgacct ataaaggcaa ctacgaacag aatttaagcg cggggattgc cgcatttcgt
    41881 accggcaatg ctccggctat tttgcaggtt tatgaagttg gcaccgccac catgatggcg
    41941 tcgaaagcca ttaaaccggt atatgacgtg tttaaagagg cggggattca attcgatgag
    42001 tcgcagtttg tgccgacggt ttcaggttac tattccgaca gcaaaaccgg ccacttactc
    42061 tcccagccgt tcaacagctc gactcccgtt ctctattaca acaaagacgc cttcaagaaa
    42121 gcggggttag acccggaaca accgccgaaa acctggcagg atctggcgga ctatgccgca
    42181 aaactaaaag cctccggcat gaagtgcggc tacgccagcg gctggcaggg ctggatccaa
    42241 ctggaaaact ttagcgcctg gaatggtctg ccgtttgcca gcaaaaacaa cggctttgac
    42301 ggcacggatg cggtgctgga gttcaacaag ccggagcagg tgaaacacat cgccatgctc
    42361 gaagagatga acaagaaggg cgatttcagc tacgtcgggc gtaaggatga atccaccgag
    42421 aagttctata acggtgattg cgcgatgacc accgcctctt ccggttctct cgctaatatt
    42481 cgtgagtacg ccaaatttaa ctatggcgta ggcatgatgc cttacgacgc cgatgcgaaa
    42541 gatgcaccgc aaaacgccat tatcggcggg gccagcctgt gggtgatgca gggcaaagat
    42601 aaagaaacgt ataccggcgt ggcgaagttc ctcgacttcc tcgctaagcc agaaaatgct
    42661 gccgagtggc atcagaaaac tggttatctg ccaatcacca aagcggcgta tgacttgact
    42721 cgtgagcagg gcttttatga gaaaaaccca ggggcggata ccgcgacgcg tcagatgctg
    42781 aataagccgc cgttgccgtt caccaaaggg ctgcgtctgg gcaacatgcc gcagatccgc
    42841 gtgattgtgg atgaagagct cgagagcgtg tggaccggca agaagacgcc acagcaggcg
    42901 ctggataccg ccgttgagcg tgggaatcag ttgctgcgcc gttttgagaa atcgacgaag
    42961 tcttaatcag tgtaatgtcg gatgcgtttc gcttatctga cctggcatcg cgtgtaggcc
    43021 ggataagcga agcgcatccg gcacagttca ggaattaacc gtaatgtcat catcccgtcc
    43081 ggtgttccgc tcgcgctggc tgccttatct gctggtcgcg ccgcagctca tcatcaccgt
    43141 tatctttttt atctggcctg cgggcgaagc gttgtggtac tcgctgcaaa gcgtcgatcc
    43201 gtttggtttc tccagccagt ttgtcggcct ggataacttc gtcgcgctgt ttcatgacag
    43261 ctactatatc gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg
    43321 tttgctggta tcgctgttct ttgcggcgct ggtggagtac atcgtgcgcg gcagtcgttt
    43381 ctatcaaacc ttgatgctgc tgccgtatgc cgtggctccc gccgttgccg ccgtattgtg
    43441 gatatttttg tttaaccccg gtcgcgggct gatcactcat tttctcgccg agttcggcta
    43501 cgactggaac cacgcgcaaa acagcggtca ggcaatgttt ctggtggtgt ttgcctcagt
    43561 atggaagcaa atcagctaca acttcctgtt cttctatgcc gcgctgcaat ccattccccg
    43621 ttcgttgatt gaagccgcag ccatcgacgg tgcagggccg attcgccgct tctttaagat
    43681 tgcgctgccg cttatcgctc cggtgagttt cttcctgctg gtagtgaacc tggtgtatgc
    43741 cttcttcgac accttcccgg tgatcgacgc cgccacgtcc ggtgggccgg tgcaggccac
    43801 cacgacgctg atttataaga tctaccgtga aggctttacc ggactggatc tggcttcgtc
    43861 tgccgcacag tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg
    43921 ctatgttgaa agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacgatattc
    43981 agccatacca tgctgatcct cgggatcgcg gtgatcctat tcccgctgta cgtggcgttt
    44041 gtcgcggcga cgctggataa acaggccgtc tacgccgcgc cgatgacgct catccccggc
    44101 acgcatctgc tggaaaacat ccacaacatc tgggtaaacg gggtaggcac aaatagtgcg
    44161 ccgttctggc ggatgttgct taacagcttc gtgatggcgt tcagcattac gctcggcaaa
    44221 attaccgtct cgatgctctc ggcattcgcc attgtctggt ttcgttttcc gctgcgtaac
    44281 ctctttttct ggatgatttt tatcaccctg atgttgccgg ttgaagtacg tatcttcccg
    44341 acggtggaag tcatcgccaa cctgaagatg ctcgacagct acgcaggttt aacgctgccg
    44401 ctgatggcct cggcgaccgc gacgtttctt ttccgccagt tctttatgac cctaccggat
    44461 gaactggtgg aagccgcgcg gatcgacggc gcatcgccaa tgcgcttctt ttgcgacatc
    44521 gtttttccgt tgtccaaaac taatctggcg gcgttgtttg tgatcacctt tatttacggc
    44581 tggaaccagt atttgtggcc gttgttgatt attaccgacg tggatctcgg caccaccgtg
    44641 gcagggatca aagggatgat cgccactggc gaaggcacca cagaatggaa ctcagtgatg
    44701 gcggcgatgt tgttaaccct gatcccacca gtagtgattg ttttagtgat gcagcgtgcc
    44761 ttcgtgcgcg gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt
    44821 aaccaaaagc tgggatggca aaacccaggt gattaaaccg ctgacccttg atgtggcgga
    44881 tggcgaattt atcgtgatgg tcgggccgtc aggctgcggg aaatcgacgc tgctgcgcat
    44941 ggttgccggg ctggagcggg tgacggaagg cgatatctgg atcaacgacc agcgggtgac
    45001 cgaaatggag ccgaaagatc gcgggattgc gatggtgttc cagaactacg cgctttatcc
    45061 gcatatgagt gttgaagaaa acatggcgtg ggggctgaaa attcgcggca tgggcaagca
    45121 gcaaattgcc gagcgcgtta aagaggcggc gcgcattctg gaactggacg gtctgcttaa
    45181 gcgccgcccg cgcgagcttt ccggcggtca gcgtcagcgt gtggcgatgg gccgcgcgat
    45241 tgtgcgcgat ccggcggtgt tcctgtttga tgagccactc tctaacctcg atgccaagct
    45301 gcgcgtacag atgcgtcttg aactgcaaca gctgcaccgt cgcctgaaaa cgacatcact
    45361 ctacgttact cacgatcagg ttgaggcgat gaccctcgcc cagcgagtaa tggtgatgaa
    45421 cggcggcgtt gccgaacaga ttggcacacc agtagaagtc tacgaaaagc ccgccagcct
    45481 gtttgtggcg agttttattg gcagcccggc gatgaatctg ctgacaggcc gcgtgaataa
    45541 cgaaggcacg catttcgaac tggacggcgg tattgtgcta ccgctaaacg gtggctaccg
    45601 tcagtatgcc gggtgtaaaa tgactctcgg cattcgcccg gaacatattg cgctaagctc
    45661 gcaggcagaa ggtggcgttc cgctggtgat ggacacgcta gagatcctcg gcgcagataa
    45721 cctggcgcac ggacgctggg gcgaacagaa gctggtggtg cgactggcgc atcaggagcg
    45781 cccgacggca ggcagcacgc tgtggctgca tctggcggaa aatcagctgc atctttttga
    45841 tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgcacatcgt
    45901 ggcggcggta agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg ggcaaaatac
    45961 ggtcataaga tgatcgaatt tgacgcgaag ttgtcgaaag atggcgaaat cttcctgctc
    46021 catgacgaca atctcgaacg taccagcaac ggctggggcg tcgcgggtga actgaactgg
    46081 caggatttac tacgtgtgga tgcaggcagt tggtacggca aagcgtttaa aggtgagccg
    46141 ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac atgggatgat ggcgaatatc
    46201 gaaatcaaac ccaccactgg caccgggcca ttaacgggca aaatggtggc gctggcggca
    46261 cgcgaactgt gggccggtat gacgccgccg ctactgtcat cgtttgagat tgatgcttta
    46321 gaagccgcac aacaggcagt accagaactg ccgcgcggtt tgttgctgga tgagtggcgc
    46381 gacgactggc gagaactgac cgcgcggctg ggctgcgact ctattcatct caattataaa
    46441 ttgcttgata aagcacgagt gatgcagttg aaagacgccg gattacggat tctggtttat
    46501 accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt
    46561 accgatgcga ttgatgtgat tggtccgaac tttacggccc aatagttttc aacggtatat
    46621 caggctgcgg cgtgccgggc tgattcagca tgtcgccgtt tctcctttcc ggcaacatat
    46681 gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg ccattggtgt
    46741 tgggcagcat ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct
    46801 gagaattatt gtttatctgg ttttctaaat gctgttgttg caactgagtt tgcgttttca
    46861 gttgctgatt cagcatccct ttttgctgga tttgctgcgt ttgcatctgg gtttgcatcc
    46921 gctgctgact ggggatctga taccccggct ggttagggtt gttcagagta ttaatgggct
    46981 gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc
    47041 ctcctctgaa gatattcttt aagtttactc ctttcctgca atcatcagga ttaattcaga
    47101 gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa
    47161 ccgacgtttt tacgccgggt ggccattgct gctctgttca caggaagttg ttttagtacg
    47221 gtagccgcgc cacctgcgcc gtctcctgtt tcttacggcg tggaggaaga tgtctttcat
    47281 ccggtacgcg cgaaacaggg gatggtggca tctgtagatg ctactgccac tcaggtgggg
    47341 gtggatatcc tcaaggaggg cgggaatgcc gttgatgccg ccgtggctgt gggctacgcg
    47401 ctggcggtaa cgcatccgca ggcagggaat ctgggcggtg gtggttttat gctgatccgc
    47461 tcgaaaaatg gcaataccac cgctatcgat ttccgcgaaa tggcacccgc caaagcgacc
    47521 cgcgatatgt tccttgatga ccagggcaac ccggacagca aaaaatcact cacttcgcat
    47581 ctggcttccg gcacgcccgg tacggtcgct ggtttctcgc tggcgttgga taaatacggc
    47641 acaatgccgt tgaacaaagt ggtgcagcca gcgtttaaac tggcacgcga tggttttatc
    47701 gttaacgacg cgctggctga cgatctcaaa acctacggga gcgaagtgct gccgaatcac
    47761 gaaaacagca aggctatctt ctggaaagag ggcgagccgc tgaaaaaggg cgacaagctg
    47821 gtgcaggcga acctggcaaa gagcctggag atgattgccg aaaacggccc ggacgaattc
    47881 tacaaaggca cgatagcgga acagattgcc caggagatgc agaaaaacgg tggcctgatc
    47941 actaaagaag atttagcggc gtataaagcg gtcgaacgca caccgataag cggcgattat
    48001 cgcgggtatc aggtttactc catgccaccg ccatcctccg gcgggatcca tatcgtacaa
    48061 atcctcaata ttctggaaaa cttcgatatg aagaaatacg gttttggcag cgccgacgcg
    48121 atgcagatca tggcagaagc ggagaaatat gcctacgctg accgctcgga atatcttggc
    48181 gacccggatt ttgtgaaagt cccgtggcag gcgctgacca ataaagccta tgccaaatct
    48241 attgccgagc aaatcgatat caataaagcg aaaccgtcca gcgagattcg ccctggcaag
    48301 cttgcgcctt atgagagtaa tcaaactacc cattattcgg tggtggataa agacggtaac
    48361 gcggtggcgg tgacctatac gctgaacacc accttcggta cgggtattgt cgcgggcgag
    48421 agcggtattc tgcttaataa ccagatggat gatttctccg ccaaaccggg cgtaccgaac
    48481 gtttacgggc tggtgggcgg tgatgccaac gccgtcgggc cgaacaaacg cccgctgtcg
    48541 tcgatgtcgc cgactattgt ggtgaaagac ggtaaaacct ggctggttac cggtagccct
    48601 ggcgggagtc ggattattac caccgtgctg caaatggtgg tgaatagcat cgattatggc
    48661 atgaatgtcg ctgaagcgac caatgcaccg cgtttccacc atcaatggtt gccggacgag
    48721 ctgcgtgtcg aaaaagggtt tagcccggat acgctcaagc tgctggaagc aaaaggtcag
    48781 aaagtggcgc tgaaagaggc gatgggcagt acacaaagca ttatggttgg gccggacggt
    48841 gagttgtacg gcgcatccga cccgcgctcg gtggatgatt taacggcggg gtactaaggt
    48901 tagcggccct cttcgtggga agagggctgt tttcttgaac aagataaatt agtgtctgga
    48961 ttgcttttac tattgctccg taatatataa tcataattat cctgaatgct tttaaggggc
    49021 ttaggttatg ctttcttttc aagagtatgc agaatagttt ttcatttacc gtgaaaatag
    49081 gacgtgcgat aattacttcc tgatattcag gaaaggaatt aactatgcta
//
LOCUS       DAPGRN010000022        48915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPGRN010000022 DAPGRN010000000
VERSION     DAPGRN010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.022"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            372..1589
                     /gene="alaA"
                     /locus_tag="RHW33_001398"
     CDS             372..1589
                     /gene="alaA"
                     /locus_tag="RHW33_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV1644921.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1673..2272
                     /gene="yfbR"
                     /locus_tag="RHW33_001399"
     CDS             1673..2272
                     /gene="yfbR"
                     /locus_tag="RHW33_001399"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV1644922.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2331..4163)
                     /locus_tag="RHW33_001400"
     CDS             complement(2331..4163)
                     /locus_tag="RHW33_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV1644923.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLMMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNSVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHIM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4250..4900)
                     /gene="hxpA"
                     /locus_tag="RHW33_001401"
     CDS             complement(4250..4900)
                     /gene="hxpA"
                     /locus_tag="RHW33_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV1644924.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4911..5405)
                     /locus_tag="RHW33_001402"
     CDS             complement(4911..5405)
                     /locus_tag="RHW33_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV1644925.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5488..5943)
                     /locus_tag="RHW33_001403"
     CDS             complement(5488..5943)
                     /locus_tag="RHW33_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV1644926.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6281..7483
                     /gene="ackA"
                     /locus_tag="RHW33_001404"
     CDS             6281..7483
                     /gene="ackA"
                     /locus_tag="RHW33_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV1644927.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7558..9702
                     /gene="pta"
                     /locus_tag="RHW33_001405"
     CDS             7558..9702
                     /gene="pta"
                     /locus_tag="RHW33_001405"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV1644928.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9892..11412
                     /gene="yfcC"
                     /locus_tag="RHW33_001406"
     CDS             9892..11412
                     /gene="yfcC"
                     /locus_tag="RHW33_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV1644929.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11445..11987)
                     /gene="yfcD"
                     /locus_tag="RHW33_001407"
     CDS             complement(11445..11987)
                     /gene="yfcD"
                     /locus_tag="RHW33_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV1644930.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12045..12596)
                     /gene="yfcE"
                     /locus_tag="RHW33_001408"
     CDS             complement(12045..12596)
                     /gene="yfcE"
                     /locus_tag="RHW33_001408"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV1644931.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12652..13296)
                     /gene="yfcF"
                     /locus_tag="RHW33_001409"
     CDS             complement(12652..13296)
                     /gene="yfcF"
                     /locus_tag="RHW33_001409"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV1644932.1"
                     /translation="MSKPAITLWSDAHCFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGTKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13432..14079
                     /gene="yfcG"
                     /locus_tag="RHW33_001410"
     CDS             13432..14079
                     /gene="yfcG"
                     /locus_tag="RHW33_001410"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV1644933.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGEIYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14136..14498
                     /gene="folX"
                     /locus_tag="RHW33_001411"
     CDS             14136..14498
                     /gene="folX"
                     /locus_tag="RHW33_001411"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV1644934.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14519..15412
                     /locus_tag="RHW33_001412"
     CDS             14519..15412
                     /locus_tag="RHW33_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV1644935.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVMTRNPQKASSVLGPQ
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTQVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15460..16362)
                     /gene="rpnB"
                     /locus_tag="RHW33_001413"
     CDS             complement(15460..16362)
                     /gene="rpnB"
                     /locus_tag="RHW33_001413"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV1644936.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16559..17332)
                     /gene="hisP"
                     /locus_tag="RHW33_001414"
     CDS             complement(16559..17332)
                     /gene="hisP"
                     /locus_tag="RHW33_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV1644937.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFSNPQSPRLQQFLKGSLK"
     gene            complement(17340..18056)
                     /locus_tag="RHW33_001415"
     CDS             complement(17340..18056)
                     /locus_tag="RHW33_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1644938.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18053..18739)
                     /gene="hisQ"
                     /locus_tag="RHW33_001416"
     CDS             complement(18053..18739)
                     /gene="hisQ"
                     /locus_tag="RHW33_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV1644939.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFLRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18829..19611)
                     /gene="hisJ"
                     /locus_tag="RHW33_001417"
     CDS             complement(18829..19611)
                     /gene="hisJ"
                     /locus_tag="RHW33_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV1644940.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19832..20614)
                     /gene="argT"
                     /locus_tag="RHW33_001418"
     CDS             complement(19832..20614)
                     /gene="argT"
                     /locus_tag="RHW33_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV1644941.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKEFAFAGSSVKDKKYFGDGTG
                     VGLRKDDTELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20880..21449)
                     /gene="ubiX"
                     /locus_tag="RHW33_001419"
     CDS             complement(20880..21449)
                     /gene="ubiX"
                     /locus_tag="RHW33_001419"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV1644942.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21544..23061)
                     /gene="purF"
                     /locus_tag="RHW33_001420"
     CDS             complement(21544..23061)
                     /gene="purF"
                     /locus_tag="RHW33_001420"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV1644943.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23098..23586)
                     /gene="cvpA"
                     /locus_tag="RHW33_001421"
     CDS             complement(23098..23586)
                     /gene="cvpA"
                     /locus_tag="RHW33_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV1644944.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24118..24780)
                     /gene="dedD"
                     /locus_tag="RHW33_001422"
     CDS             complement(24118..24780)
                     /gene="dedD"
                     /locus_tag="RHW33_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV1644945.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVVPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24770..26038)
                     /gene="folC"
                     /locus_tag="RHW33_001423"
     CDS             complement(24770..26038)
                     /gene="folC"
                     /locus_tag="RHW33_001423"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV1644946.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKTLPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26108..27022)
                     /gene="accD"
                     /locus_tag="RHW33_001424"
     CDS             complement(26108..27022)
                     /gene="accD"
                     /locus_tag="RHW33_001424"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV1644947.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27178..27837)
                     /locus_tag="RHW33_001425"
     CDS             complement(27178..27837)
                     /locus_tag="RHW33_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1644948.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27920..28732)
                     /gene="truA"
                     /locus_tag="RHW33_001426"
     CDS             complement(27920..28732)
                     /gene="truA"
                     /locus_tag="RHW33_001426"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV1644949.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28732..29745)
                     /locus_tag="RHW33_001427"
     CDS             complement(28732..29745)
                     /locus_tag="RHW33_001427"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV1644950.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFVQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29811..30947)
                     /gene="pdxB"
                     /locus_tag="RHW33_001428"
     CDS             complement(29811..30947)
                     /gene="pdxB"
                     /locus_tag="RHW33_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV1644951.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGQYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31046..32041
                     /gene="flk"
                     /locus_tag="RHW33_001429"
     CDS             31046..32041
                     /gene="flk"
                     /locus_tag="RHW33_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV1644952.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSTLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32038..33216)
                     /locus_tag="RHW33_001430"
     CDS             complement(32038..33216)
                     /locus_tag="RHW33_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HDV1644953.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33491..34711)
                     /gene="fabB"
                     /locus_tag="RHW33_001431"
     CDS             complement(33491..34711)
                     /gene="fabB"
                     /locus_tag="RHW33_001431"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV1644954.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34870..36876
                     /gene="mnmC"
                     /locus_tag="RHW33_001432"
     CDS             34870..36876
                     /gene="mnmC"
                     /locus_tag="RHW33_001432"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV1644955.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRSDLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLSLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSDEDQQQNRQRLIDCFPQAQWAKEVDISEKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDKAVSAPVYDDLFMLAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36997..37275)
                     /locus_tag="RHW33_001433"
     CDS             complement(36997..37275)
                     /locus_tag="RHW33_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV1644956.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37309..37857)
                     /gene="epmC"
                     /locus_tag="RHW33_001434"
     CDS             complement(37309..37857)
                     /gene="epmC"
                     /locus_tag="RHW33_001434"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV1644957.1"
                     /translation="MNSTHHYEQLIEIFNSCFADDFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDARTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPARPARFIKA
                     LQNYYHTPEITAEQFPWPEALN"
     gene            complement(37857..38666)
                     /locus_tag="RHW33_001435"
     CDS             complement(37857..38666)
                     /locus_tag="RHW33_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV1644958.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFIGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38666..39490)
                     /gene="mepA"
                     /locus_tag="RHW33_001436"
     CDS             complement(38666..39490)
                     /gene="mepA"
                     /locus_tag="RHW33_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV1644959.1"
                     /translation="MNKTAIALLALLASSVSLAAAPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39494..40579)
                     /gene="aroC"
                     /locus_tag="RHW33_001437"
     CDS             complement(39494..40579)
                     /gene="aroC"
                     /locus_tag="RHW33_001437"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV1644960.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAETFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40614..41546)
                     /gene="prmB"
                     /locus_tag="RHW33_001438"
     CDS             complement(40614..41546)
                     /gene="prmB"
                     /locus_tag="RHW33_001438"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV1644961.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            41712..42263
                     /gene="smrB"
                     /locus_tag="RHW33_001439"
     CDS             41712..42263
                     /gene="smrB"
                     /locus_tag="RHW33_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV1644962.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42431..43273)
                     /locus_tag="RHW33_001440"
     CDS             complement(42431..43273)
                     /locus_tag="RHW33_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV1644963.1"
                     /translation="MKLLRNFMGLLTLMVLSLTAQAESFHMETPMGYPSAFSRIVFSL
                     EVVTASGVYTGTYQSSKPPLLVVGNLTLLSWNGPSSIPAPTIYLNHSDNTSMSNCPGL
                     SSVPGTDGSWECIMAELEVTVTGDAQGCPWLVSSQITTTDLGSVGATYVGPKVHNSTC
                     PSIPLTTYDVSWDENTVAHKKTLNLQSTGGIIETTLSTYLMESQKLCDGSLFADRGAS
                     CRFVSQMITFTASGCDDAKVTVTPVPHPITDRQLHDIVVRVDTSALQPIDSTCRFQYV
                     LNMF"
     gene            complement(43275..43799)
                     /locus_tag="RHW33_001441"
     CDS             complement(43275..43799)
                     /locus_tag="RHW33_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644964.1"
                     /translation="MKTKTGCMLLTGLLLAASGTTLAADNLHFYGNLLSKSCTLVVDG
                     ELLAEVHFPTVSSQDLMVAGQSSRVPVVFKLKDCKGPADYQVKVTLTGTEDSEQPGFL
                     ALDPASGAQGVGIGMEKSDGTWVPLNDSAGATFNLSNGANNLNFNAWLQAKSGRDVTS
                     GDFTASLTATFEYI"
     gene            complement(43796..44281)
                     /locus_tag="RHW33_001442"
     CDS             complement(43796..44281)
                     /locus_tag="RHW33_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644965.1"
                     /translation="MKRLALLLICGVCGGVQASPDITFHGMLVSPPACTINEGRTIEL
                     EFRDVLIDSIDGNYARQEVPYTIKCDSNVRNEAWEMTLTWTGTQTSYNDAAIETDVPG
                     FGIQLLQNGQRFQLNTPLTINATDLTQKPKLEAVPVKASGVTLNDGAFSAYATLRVDY
                     Q"
     gene            complement(44278..44769)
                     /locus_tag="RHW33_001443"
     CDS             complement(44278..44769)
                     /locus_tag="RHW33_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1644966.1"
                     /translation="MRRILLAGSGIALLAWNVASAETRPVDLELRVRVDAPPPCTVTG
                     SAVEFGDVLIASIDGNNYRQNAGYTLDCSGRLEDDLRMQLKGTTTTVNGETVLDAGIA
                     GFGIRIENAADNSLFSIGENNWTPFAFNSLPQLKAVPVKQNGVQLAPAEFNASMTMVV
                     DYQ"
     gene            complement(44785..45534)
                     /locus_tag="RHW33_001444"
     CDS             complement(44785..45534)
                     /locus_tag="RHW33_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1644967.1"
                     /translation="MTNLFSAKTVATTLALLITATSLQAQASVTPDRTRLIFNESDKS
                     ISVTLRNNDPKMPYLAQSWMEDEQGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDIN
                     KLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENVSMKNPWQHKVT
                     LTRSGQEFTVNNPTPYYVVISNASAQKNGNPAAGFSPMVISPKTSVPLNVKMDSVPVL
                     TYVNDYGARMPLYFKCGGDTCQVDAEQSRKG"
     gene            complement(45557..48196)
                     /locus_tag="RHW33_001445"
     CDS             complement(45557..48196)
                     /locus_tag="RHW33_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1644968.1"
                     /translation="MPDHSLFRLRVLPCCVALAMSGSYVNAWAEDEIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLTEEYDIYWYASENDASKTYACLTPELV
                     AQFGLKEDVAKNLQWIHDGKCLKPGQLEGIDIKADLSQSALVISLPQAYLEYTDINWD
                     PPSRWDDGISGLIADYSITAQTRHEENGGDDSNEISGNGTVGVNLGAWRLRADWQTDY
                     QHSKSNDDDVINGDDTLKNWEWSRYYAWRALPSLKAKLGLGEDYLNSDIFDGFNYVGG
                     SISTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDINTASMPFLTRPGQVRYKLMMGRPQEWGHHVEGGFFSGG
                     EASWGIANGWSLYGGALADEHYQSAALGVGRDLSVFGAVAFDITHSHTRLDKETAYGK
                     GALDGNSFRVSYSKDFDELNSRVTFAGYRFSEENFMTMSEYLDASDSEMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDRDEQTNYNVMLSHYFNLGSIRNMSVSMTGYR
                     YEYDNQTDKGVYISLSMPWGDSSTISYNGNYGSGSDSSQVGYFSRVDDATHYQLNVGT
                     SDNHSSVDGYYSHDGSLAQVDLSANYHEGQYTSAGISLQGGATLTAQGGALHRTQNMG
                     GTRLLIDADGVAGVPVEGNGAAVYTNMFGKAVVADVNNYYRNQAYIDLNNLPENAEAT
                     QSVVQATLTEGAIGYRKFSVISGQKAMAVLRLQDGSHPPFGAEVKNDNAQNVGLVDDD
                     GNVYLAGVKPGEHMIVSWGGVAHCDIHLPDPLPADLFNGLLLPCQQTGAISSPVHNEI
                     KPVIQEQTQQVTPTEPPVSVSAN"
     gene            complement(48278..48841)
                     /locus_tag="RHW33_001446"
     CDS             complement(48278..48841)
                     /locus_tag="RHW33_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1644969.1"
                     /translation="MSKFVKTAIAATMVMGAFASTSTIAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGSGILKNNGTSTPKAFQIHLQDCVFDTQTTMTTTFTGNASSTN
                     SGNYYTIYNTDTGAAFNNVSLAIGDAQGTSYKSGAGIEQKIVNDTATNKGKAKQTLDF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11899 a        13273 c        12304 g        11439 t
ORIGIN      
        1 tagtttcaca ccttgccccc attgcgacac ctcggaaatc atcacgctgt taaactctcc
       61 gtaatgcgga aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac
      121 acaattatct tacagataaa aaaaccatac ttacaattaa gaatcagaac aattcactat
      181 ataacattgc atgtaaagca tatacacctc attattttgt cattattaag ttattaacag
      241 cacaatcgag ccctcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct
      301 gggggagtac attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa
      361 ggtcaagatt catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca
      421 tccgtggtcc ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga
      481 aactgaacat cggcaaccca gccccgttcg gttttgacgc gccagatgaa atcctcgttg
      541 acgtgatacg caacctgcct accgctcaag ggtattgcga ttccaaaggt ctttactccg
      601 cgcgtaaagc catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag
      661 atatttacat cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga
      721 acagcgggga cgaaatgttg gttcctgcac cagattaccc actatggacc gcggcggttt
      781 cgctttccag cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg
      841 acctcgatga tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc
      901 caaataaccc aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg
      961 ctcgtcagca taatctcatt atcttcgccg atgaaattta tgacaaaatt ctctacgacg
     1021 acgctgagca tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaatg
     1081 gactgtcgaa aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc
     1141 cgaaaaaaca cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt
     1201 gtgctaacgt tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca
     1261 gtgaatttat tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga
     1321 tcaacgatat tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga
     1381 aaatcgacgc caaacgcttt aacattcacg acgatcagaa aatggtgctg gatttcctgt
     1441 tgcaggaaaa agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact
     1501 tccgcattgt cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc
     1561 gtttcctttc tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg
     1621 cagtgttatc ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca
     1681 gagccatttt tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctcatgcg
     1741 caacgtgcgg acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc
     1801 gctggcagct atcaaaaatc ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt
     1861 actggcgatg taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa
     1921 atacttcaat tcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa
     1981 actggtcgat atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca
     2041 tgcatatagc gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct
     2101 gaaatgtctg gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact
     2161 ggaagcgacg cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt
     2221 tcccagcttc catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg
     2281 agtctgcgtc gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga
     2341 aacagcatcg ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc
     2401 aacttcacaa agtcactgaa gctgtaattt cccggaccta aaaccagtgt gttaacaggt
     2461 gaagaaaccg gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcgaatgga
     2521 taaggcgaca cccccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc
     2581 gtcgcggtat tagagataaa tagcccaata accgccgaca agacaaacaa acaacccagc
     2641 attatatgtg gcccataacc gccgccaatg tccatcagcc ctttcaccgc cagcgcgaca
     2701 cctcccgttt tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc
     2761 ggccagtgaa tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc
     2821 aggcaggcga tgatagcggc aacaggatta ggaatttcat ctgtcagcat taacgccacc
     2881 attagcacca gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc
     2941 acctcttccg gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc
     3001 agtttccagt tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc
     3061 gaaccttcca gcgccacgct attgcgcttt agccccacca cattcagtcc gtagcgggta
     3121 cgaaaaccaa tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc
     3181 tctgccatgc ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc
     3241 tccagcaatt gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata
     3301 agcaaaacgt cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga
     3361 aaacgccgcc agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga
     3421 tcgtccagcc gttgaccaat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc
     3481 ccggtcagtc gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc
     3541 tgcggggtct gggtatcccc tttcagcatg aaacgcatca ttaacatata caaaataccc
     3601 agcaccagca cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct
     3661 tcacgcagca attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg
     3721 ccgctaatca gcccggcaaa acttaacggc atcatcagac gtgacggcga cgtttgcata
     3781 cgcatggcaa cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata
     3841 aacgcgccaa gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg
     3901 cccgcaactt tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca
     3961 tcgccaataa taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat
     4021 acttctggga ctgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc
     4081 gcatccatac gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga
     4141 acccaaatca attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc
     4201 tgccataaaa aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa
     4261 taacatcgcc atttggctgt ttagtcacag taatttgctc cagactgtgg aggaccaaat
     4321 cgacctcatt caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg
     4381 ccgccaggcc agaaagcacg ccagcgggag catcttccac caccacacac tcctgcggcg
     4441 caagccccag cagctgcgcg cctaacagat acgcatcagg ttctggtttt ccgcgcttca
     4501 ctcgctcagc ggttacaaac acctctggtg cgggaagccc agctatttta tggcgcgctc
     4561 gcgctaccgg catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat
     4621 gattgagtaa ggcgattgcc cccggaagcg cagtaatacc ttcggtttcc gtggcctcga
     4681 tttgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat
     4741 ggcgcagaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta
     4801 acccatgacg tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca
     4861 gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg
     4921 ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc atgccacaca
     4981 ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc gtgagtgccg
     5041 gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa
     5101 cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc
     5161 tggtcctgca aattagacca ggaaacatgc aacgcatgat acatctccat aatgtcgatg
     5221 atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccagttc acgcatctgt
     5281 aatccataac cacgctcaat gattgtttgc aggcgacggt aacgttcagc atttgccgga
     5341 tcgagcatag tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttagtcatt
     5401 tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct
     5461 tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg
     5521 tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg
     5581 tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc agttgaggat
     5641 agcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct gcatgggtaa
     5701 acttaaggcg aacagcgcag tggcaactgc cggcccaagc tgaccgccca gggcaatctg
     5761 ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt
     5821 gataacgcga ttttcgacaa agaccggggc aaggcgtttt tccagcggcc acgtctttga
     5881 gtaatgctgt ccccggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga
     5941 catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag
     6001 cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc
     6061 tgaaggccta agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg
     6121 ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt
     6181 tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc
     6241 ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact
     6301 ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga
     6361 agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
     6421 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct
     6481 caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc
     6541 tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga
     6601 gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca acccggctca
     6661 cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc
     6721 tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc
     6781 gtacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
     6841 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat
     6901 cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga
     6961 cacctctatg ggcctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat
     7021 cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa
     7081 caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg
     7141 ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg
     7201 ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
     7261 tgttgtattc accggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg
     7321 caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg
     7381 caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga
     7441 agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc
     7501 agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg
     7561 tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt
     7621 ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct
     7681 cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct
     7741 tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc
     7801 aatcagaaag atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac
     7861 gctgaagtcg ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct
     7921 ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc
     7981 accgacaccc cagaacagct gaaagagcgt atcgaactga cccgcaacag cttcggcggt
     8041 gccaaaaaca ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa
     8101 cagggtcgta ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta
     8161 aacaatgttg atccggcgaa gctgcaagaa tccagcccgc tgccggttct cggcgctgtg
     8221 ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg
     8281 accatcatca acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca
     8341 cgcagcattc cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca
     8401 gaccgtcctg acgtgctggt tgccgcttgc ctggctgcca tgaacggcgt agaaatcggt
     8461 gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt
     8521 gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca gacttctctg
     8581 agcctgcaga gcttcaacct ggaagttcca gttgacgatc acgagcgtat cgagaaagtt
     8641 caggaatacg ttgctaacta catcaacgct gactggatcg attctctgac tgccacttct
     8701 gagcgcagcc gtcgtctgtc tccgccagcg ttccgttatc agctgactga acttgcgcgc
     8761 aaagcgggca aacgtatcgt actgccggaa ggtgacgaac cgcgtaccgt taaagcagcc
     8821 gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc
     8881 aaccgtgttg cagcgtctca gggtgtagaa ctgggtgcag gcattgaaat cgttgatcca
     8941 gaagtggttc gcgaaagcta tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg
     9001 accgaaaccg ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg
     9061 gaacaggatg aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc
     9121 cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc ttctgtgttc
     9181 ttcatgctgc tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg
     9241 accgcagaac agctggcaga aatcgcgatt cagtccgctg attccgctgc agccttcggt
     9301 atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac
     9361 gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc
     9421 gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc taaagcgccg
     9481 aactctccgg ttgcaggtcg cgctaccgtg ttcatcttcc cggatctgaa caccggtaac
     9541 accacctaca aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag
     9601 ggtatgcgca agccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac
     9661 accatcgcgc tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccgcag
     9721 ctttgcgctg cggatatctg aaccggaaat agtcactatt tccggttttt tattctctta
     9781 atttgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca
     9841 tttcactttg catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca
     9901 atcactgaat ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt
     9961 tttgttgcta ttttaaccag ccttgccacc tgggtagttc cggtggggat gtttgacagt
    10021 caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac
    10081 tcatttcgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc
    10141 acgacgggcg atgaacgccc gggcctgatg aacttcccgt ttgaagggtt aacctcagga
    10201 tcgaaatacg ggacagccgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt
    10261 ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc
    10321 cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg
    10381 gtatttggta tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc
    10441 cggctgggct atgacagtat taccaccgtc ctggtgacct atattgccac gcaaatcggt
    10501 tttgccagtt cgtggatgaa cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt
    10561 ccggtgcttt ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tctgattggc
    10621 ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg
    10681 catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc
    10741 tttggtgact ggctggtatt gattgtcctg accgccgtaa tggtctgggt gatttggggc
    10801 gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg
    10861 gtgattggca tcatcggcgt tgttttccgc cttaacggca tgacggttaa taccatggct
    10921 tcatccttta ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc
    10981 aaagggattt tgctgctggt tggtaatggt gaagcaggtg atgccagcgt gttaaatact
    11041 atcctcaaca gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt
    11101 atgttgctct tccaggcggt atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg
    11161 ttaaccatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc
    11221 gtgctggcgt tccagtttgg tgatggcttc agtcacatca tttacccaac ctcagcttcg
    11281 ttaatggcaa cgctcggtgt ttgccgggtg gacttccgta actggctgaa ggtgggcgcg
    11341 accctacttg gactgctgtt tattatgtcc agcgtcgtgg tgatcggcgc tcagttgatg
    11401 ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca
    11461 gtctctacgg cttcattttt ggcattgcgc ttcatccaca acgctagcgc tttcagcgag
    11521 tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca
    11581 ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcagctgaa caatgcgccc
    11641 cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg
    11701 gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc
    11761 tgaaccacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta
    11821 cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga
    11881 cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca
    11941 ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct
    12001 tctttttctg gcgctcttac ggcgcattat gagttgtggg taaattacgg attaatcgcg
    12061 acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc
    12121 atgccataac tcgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg
    12181 aaaatctccc cccgctgttc tgccactggc agatgggtat ggccgtacac cagcacatcg
    12241 ttctggttta aagcaggcag attttccggg ccaaaaagat gaccatgcgt caaaaacaga
    12301 cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt
    12361 tgatccactt cgctgtcgca gttgccgcgc acagcgataa ccttatgtgc cacttcatta
    12421 agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga
    12481 ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttgggcaaa caactccaga
    12541 acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact
    12601 cactcctcgt cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga
    12661 ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgttgc
    12721 ataatccacc aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag
    12781 cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtactaa
    12841 caaatgttct gccatcgcga acagtttctc tgcactggct tttccctcgg ccgttagtgg
    12901 cgcttttttc gtccccgcaa agacaacatc cgtcggacgc tcttcgcgga tgggcatcag
    12961 atcgctgcgc agccaggcct gaatctgtcg tgcacgtgca cgattttcta aatcaagcgg
    13021 ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg caatggcaga
    13081 agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg tctgaccgta
    13141 accttgccac gtcggttgca aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa
    13201 cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg agaaacagtg
    13261 ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa taaagggggc
    13321 tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg
    13381 ccgcgctttc tatacttgtg gtgttcagat gattagacaa ccggagctgc aatgatcgat
    13441 ctctatttcg ccccgacacc caatggccac aaaattacgc tgtttctcga agaagcaggg
    13501 ctggattatc gcttgataaa ggtagacctg gggaaaggcg gtcagtttcg cccggaattt
    13561 ttgcgcattt cgcctaacaa caaaattccg gcaattgttg atcattctcc agccgatggc
    13621 ggcgaaccgc taagcctctt tgaatctggt gccattttgt tgtatctggc tgagaaaacg
    13681 gggctctttt tgagtcatga aacgcgtgaa cgcgccgcca cattacagtg gttattctgg
    13741 caggtaggcg gactggggcc gatgcttggg caaaatcatc attttaatca cgcagccccc
    13801 caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct gtaccatgta
    13861 ctgaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agatctacag cattgcggat
    13921 attgcctgct ggccgtgggt taatgcctgg actcgccagc gaattgacct cgcaatgtat
    13981 ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg
    14041 ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt tctcgtgtat
    14101 tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc gctattattc
    14161 gtataaagaa ccttcgttta cgtacattta tcggaattaa ggaagaagaa attaacaacc
    14221 gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg cgcactagcg
    14281 aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt cagcatgtag
    14341 agaataaccg tttctctttg ctggaaaaat taactcagga tgtgctcgat atcgcacgtg
    14401 aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg ctgcgctacg
    14461 ccgattcggt atccatgacc ttaagctggc agcgttaacc gccatatcgg gaggctgcat
    14521 gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc cacgtttgct
    14581 ggagctgggc catcaaatca cggtaatgac gcgtaacccg cagaaagcca gttccgtgct
    14641 cgggccgcag gtgacgctat ggcaagggct tgccgatcaa agcaacctca acggcgttga
    14701 tgcggtaatc aacctcgccg gagaaccgat tgctgataaa cgctggactc acgagcaaaa
    14761 agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa ctagtcgatt tgattaatgc
    14821 cagcgacacg ccaccgtcgg tactcatttc cggctcggca acgggctatt atggcgactt
    14881 aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttaccc ataaactctg
    14941 cgcccgctgg gaagaaattg cctgccgggc gcaaagtgac aaaacgcaag tgtgcctgct
    15001 gcgtaccggt gtagtgctgg caccggatgg cggtattctc ggtaaaatgc tgccgccgtt
    15061 tcgtcttggc ctgggcggac cgattggctc tggtcggcag tatctggcct ggattcatat
    15121 tgatgatatg gtcaacggta ttctctggct gctggataac gagctgcgcg ggccatttaa
    15181 tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg gtcatgcgct
    15241 gcatcgcccg gccattttgc gcgtccccgc gaccgccatt cgactgttaa tgggcgaatc
    15301 ttcagtactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag aagcgggttt
    15361 tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct gatgtggttt
    15421 actgcaaaca tccgccagtt aactcccggt gttacagggt tagtggcttt gcgcgataag
    15481 atcgtctggt gaaagtcggg tcaccatcat aactaactct cggtctaaac ctctatccag
    15541 catctcctga gcaatacgca gggcttcttc gcgtttgcca agaattaatc catcattccg
    15601 cccttcttca cgtaatctgt cagcaatggt catcagtttc tccttttcct gtggtgcgcg
    15661 ttctgctatc tcaccaataa atgcacggaa acgctgggca tctcccgttt gtaatacata
    15721 attaaacagg gcttttagct gtctgtcatt agtgttccct gtaactagca gcgaaacaat
    15781 ttggtcgact aatcccaaca gatcgcgctg acgaatatgt ttctgaatta actccaacag
    15841 cgccattttg cgatgttgca taatctcgtc atccggcacc acggtaatat ccaccaacgg
    15901 aaaagccgat gaatatattt tacgggcaat cgcaggctcg gcaaattcat caagccagca
    15961 gagtgaataa ggataaggac ttctgcaacc atgataaaac agcatcggga tcaccaatgg
    16021 aagctcttta tagcccgcat caagatggtt ttgcattgcc gcaatggaat aacgcatcat
    16081 gcgaaaagcc attaattctt ccggcttact ttggtgctct atcactacat aaatataacc
    16141 cactccctcc tgcgttttca cagaccacaa gaggtcggaa taatattgcc gcaggtcttc
    16201 atcaataaaa ctgtttggtt ccagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg
    16261 cgcgggaaga tgaatatcaa taaaatcccg cgcggtgtct ggatggcgta aaaaagattt
    16321 aaataccgca tcatgcggcg tggaagttgt cgatattgtc atggcgttcc gtcacccctc
    16381 aaatttaaga tgacgcgaca ataaccacga ttaacaccca atgcagcagg cagtttttat
    16441 ctttggacag cgaattcaga agtattttta gcggactgca acttttacat ttcagcggaa
    16501 gctgactcgc aaattcccct tgaaaatact ccggcagcgt tagccaccgg agaggcaatt
    16561 atttcagcga acctttaagg aactgttgca gacgagggct ttgcgggttg ctaaataact
    16621 gctccggcgc gccttcttcc tctattttcc cctgatggag gaaaatgacg tgagtagaaa
    16681 catgacgagc aaagcccatt tcgtgagtca ctaccaccat ggttttcccc tcttctgcca
    16741 gttgctgcat aatgcgcaac acttcgccta ccagttcagg atcgagcgcc gaggtaggtt
    16801 catcaaacag taaaacttcc ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt
    16861 gctgaccgcc ggaaagatgc accggatatt tcccctgtgc acgttcgtct atcccgactt
    16921 ttgccagata cttcaccgcc cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa
    16981 tcggcgcttc catgacgttt tccagcaccg tcatatggct ccagagattg aagtgctgga
    17041 ataccatcgt caggcgtgtg cgcagtaagc gcagttgatt tttatcggcg actttgagtt
    17101 gaccgtcttt gtcgcgcacc agattgatcg tctggccatt gaccacgatc gacccttcac
    17161 tcggtttttc gaggaagtta atgcagcgca gaaaggtact tttccccgat cccgacgatc
    17221 cgatgatact tatgacatct ccggcattcg cttgcagtga gacccctttc agcacttcgt
    17281 gttcgccgta gcgtttgtgc aaatcgataa cgtttaattt attctcggac atcgtattct
    17341 cagtgcgttg aagaaggttt cacatgctgc aaccagcgtt tttccgctct gcgaaagagg
    17401 ctgatcagga cataagagat gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa
    17461 ggttgatacg tggcggcgtt aatatcgcgg gctattttca gcagatccgg cacagtggca
    17521 gtaaacgcca gcgcagtaga atgcagcatc aggatcactt cgttgctgta tgccggtaac
    17581 gcaatacgca gcgcagaagg caaaataatg cagcgataca ttttaaaggt tgagaaaccg
    17641 taggctctgg cggcttcaat ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc
    17701 tcggtagtgt aagcgcaggt gttaagcgtc agcgccagca cggtacagtt caggccactg
    17761 cggaaaaagg cgttaagaaa ctcggtcccc ttaacaatct ccagcgtata catgccggaa
    17821 tagaacacca gcaactgaac atacagcggc gtaccgcgaa aaatataggt aaataaccag
    17881 attggaaact ggatgtattt attactggag acacgaccaa tcgccagaaa cagcgccagg
    17941 actccgccta tcactaccga caaaataagc agccacagag tgatcgccac accagtaaag
    18001 cgataaccgt cggtccacag cagcggtttc cagtattcat gtaagatttc gatcacaggt
    18061 cagccctctt cacacccacg gagtagcggc gctcaaggaa cagcagcaca ccattggaaa
    18121 cggtggtgaa aaccaggtaa atcacgccac agacgatggc gaaatagaac ggttcccagg
    18181 tacttttgcc tgccagctgc gtggctttga ccacatcttc caggccgagt aacgaaacca
    18241 gtgcggtaga tttgaggatc acctgccagt tgttgccaat gcctggcagg gcgtaacgca
    18301 tcatcgccgg aaacatgatc cgcagaaaca cttgcccacg agtaaaaccg aacgccgtcg
    18361 ccgcctctat atgtcctttc ggcactgcca taaaagcacc acggaacgtt tcggtaaaat
    18421 aagcaccgta gataaaaccg agagtgataa taccagcgac catcggatcg atatcaatct
    18481 gcccgacgcc catcgcctcc gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca
    18541 gcagcatcaa cactaagtcc ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca
    18601 gccccgaaag ccgattttgc gagagcttac cgccagcgcc aattaaaccg atgattacag
    18661 cgagcactac agagctgata gccagctcca gcgtgacgag cgcaccctgt aaaataacac
    18721 ctgaaaaccc atacaacatg cagcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt
    18781 ctccctccgg ttacccgaag ggagcgggct ttctttcacc gatggtgatt agccaccata
    18841 aacatcaaaa tcgaagtact ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc
    18901 agcaaaggct ttgttcagtg cctcgcgcag ttcgttatct tctttacgca ggcccatgcc
    18961 ggtccctacg ccaaacagtt tttcatcttt aacagacggg ccaccgaatt tgtaatcttt
    19021 accgacaggt tgtttgagga aaccttcgct ggcagcgacc tcatcctgga acgcggcatc
    19081 aatacgtccg gcagtcaggt cagaataaat gttgtcctgc ccctgatacg agacgatttc
    19141 aatgcctttt ggtgcccaat gttcattacc gaacgtctcc tgggtggtgc cctgcaatac
    19201 gcctacccgt ttgcctttca gcgactcgac tgtcggctga atgtcagaat ttttcgccac
    19261 caccagacga gaatcagcag cgtacagttt gtcggtgaag gctatttctt gctgacgttt
    19321 ttccgtaatg gaaagcgatg acatgatggc gtcaatcttc ttcgctttta aggacgggat
    19381 taacgcatcc agcggatttt cgacaaacgt acattgcgta ttgatgcgtt tgcataattc
    19441 ctttgccaga tcgatatcga agccaaccag ttcgccttgt gaattttttg attcaaatgg
    19501 cgcataggtc gggtcggtac cgatgcggat gttttgcgga atcgcagcaa atgccgcagt
    19561 tgcgctggag aaggccagaa ccagagagag cgatagcacc agttttttca taatgtcctc
    19621 aactgacagt ctttttatag ggaatattta caggtttgtt acacttatcg tgccataaaa
    19681 ttgaccatca aggcaaaata tcccgccccg ttagacgtat tatttttcat taaaaatgct
    19741 taagtatgca tttcaatgca ccacgatagt gcaacaaacc tggcgtgcac catgatgatc
    19801 attccatcag gcacagcttc cctgcgacgt atcagtcacc gtagacatta aagtcgaaat
    19861 actttttcgc catcttgtcg taggtgccgt cctgacgcag ctctccaagc gccttattaa
    19921 acgcagccgt cagttcagta tcatctttac gtagccctac accggtgcca tcaccgaagt
    19981 attttttgtc tttcactgat gagccagcaa aagcgaactc tttaccggcg ggttgcttga
    20041 ggaaaccttc gctggccgcc acttcatcct gcagtgcggc atccagacgt cctgcagcca
    20101 gatcggaata gaccaaatcc tggttggcat aggccaccac atccacgcct ttgctacgcc
    20161 aggtctcgtt agcgtaagct tcctgggttg atccctgcag cacacccaca tgtttacctt
    20221 tcagtgaatc cagcgttggc tgaatcggcg aacctttggc ggcaatcaaa cgagaatctg
    20281 cggcgtacag cttgtcggag aaggcaatct cctgctgacg tttatcggta atggaaagcg
    20341 acgaaataat ggcgtcgatt tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac
    20401 tggcaaccca ggtacatttc acctgcatcc gtttgcacat ctcgttaccg agatcgatat
    20461 caaagccaac aaaatcacct ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg
    20521 ttccgatacg taccgtctcc ggtagcgccg catagctgga agccgctgcg gagagaccga
    20581 ctaacaaaga cagagcgaga atcgacttct tcatacataa ccctcaagtg aaaatggctt
    20641 ttttatgttg tgtattgtgt tgtgtgtttg caggctcttt catgcaggtc ttatgccatc
    20701 ttgacgacaa cagtaacatt caacgttaaa tatgttaata agacgttgca ttattgtcct
    20761 aaagttgaag atagcaggta tagcggttga atcgcagcgt ttcgattgaa tggcagaaac
    20821 aaaatgtcga ggatttgatc gcggttgcaa aattgccctg aaacagggca acagcggagt
    20881 tatgcgccct gccagcgggc aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga
    20941 acacgattaa ccgtctgatt tatcacatca tcaagggatt gcgggcgatg ataaaacgcc
    21001 ggaacgggag gcataatcac cgcaccgatt tctgctgcct gcgtcattaa acgcagatgg
    21061 cctaagtgca atggtgtttc acgcacgcag agcaccaacg ggcgacgctc tttcagcaca
    21121 acatctgccg cacgggtcag taagccatca gtatagctat ggacaatgcc ggacagggtt
    21181 ttgattgaac agggtaaaat caccatcccc agtgtctgga aagaaccgga agagatgctg
    21241 gcggcaatat cgcgcgcatc atgcgtgaca tcggctaatg cctgcacttc gcgcagggaa
    21301 aaatccgttt cgagggataa ggtctggcgc gctgcctggc tcatcaccag atgcgtttcg
    21361 atatctgtga catcgcgcaa aacctgtaat aagcgcacgc cataaatcgc gccgctggca
    21421 ccgctgatgc ccacaataag tcgtttcata aatagttgcc ctgtcagact tgcaggcaga
    21481 ctttgcagga tttcggcggg agttgcaagt cagggtgcca gaccggcacc ctgagcgaag
    21541 gcatcatcct tcgttatgca tttcgagatt ttccacttcg ttctgacgtt gcactgcttt
    21601 ggcgtcgtca ttacgtaacg tatcgaggaa atcgaggtag ccctgatcaa catctttggt
    21661 gacgtagacg ccgttgaaca ccgagcattc aaactgctgg atatccgggt tttcagcgcg
    21721 aacggcgtcg atcagatcgt tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg
    21781 gcgaatttca tctacttcgc gaccgtgagc gatcagttcc gtagcgctcg gcatatcgat
    21841 accgtaaacg ttcgggaagc gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc
    21901 tccggcttcg cgtgccatct cgataatctg ctcagaagtg gtgccacgga cgatggagtc
    21961 gtcgaccagc aggacgtttt tatcgcggaa ctcggcgcgg ttggcgttca gtttacgacg
    22021 cacggactta cgacgcagct gctggcccgg catgatgaag gtgcggccaa catagcggtt
    22081 tttaacgaag ccctggcggt acggtttgcc cagaatacga gcaatttcca gcgcgatatc
    22141 acacgaggtt tccgggatcg ggatcaccac gtcgatatcc agatcttccc attcgcgggc
    22201 aattttctcg cccagtttcg tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc
    22261 gatgaacgag tccgggcgag caaagtatac atactcaaac aggcacggat tgctgaccgg
    22321 attgtcagca cattgacggg taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc
    22381 tggcgcgaca tcacgcagga aatcaaagcc cagtgtatcg agcgctacgc tttcggaagc
    22441 gaccatatat tctgtgcggt tctcgtcaat atcacgtttt cccagaacca gcggacgaat
    22501 accgttcgga tcgcggaaag caaccatacc gtggccgata atcatcgcca cacaggcata
    22561 cgcgccgcgg attaagcggt ttgtagcagc gatggcggcg aaaatgttgt cggcttccag
    22621 cgggtagtgg cggaagttgt ccagctcgct ggcgaagata ttaagcagaa tttccgagtc
    22681 ggaagtggtg ttgatgtggc ggcgtttttc ttcaaacagt tttttacgca actcgtgagc
    22741 gttggtcaga ttgccgttgt gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg
    22801 ctgcgcttca gaggcgctgg agctgccagc cgtggggtaa cgcacatgac caatgcccat
    22861 attgccctgc aaacgctgca tatggcgagc ttcaaataca tcgctcacca gcccgttcgc
    22921 tttacgcaaa cggaagcagt tattggcatc tatggtgatg atgccggcgg catcctgacc
    22981 gcgatgctga agcaccgtta aggcatcata aatcgactgg ttaaccggca taacaccggc
    23041 gataccgaca ataccgcaca tacgtctttt tcctcgttaa gccacatctc agagcactta
    23101 cgctctgggc aagaaacttg acgagctttg cagataatca aaaaaccatc tgatgataaa
    23161 actgaactgc gggatcagct gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa
    23221 ggagtcgaga aagaagagaa tggcagcaac aatcaacaca ccgcgcaacg caccgaaaca
    23281 gacgcccagc acccgatcgg tgcctgacaa ccccgttttc tccaccagct ggcctatcac
    23341 gaagttcacg atagcaccaa cgatcagggt agcgataaac agtaccgcga tggcaatccc
    23401 atttcgaacc agttcgtctt caaagcccgt aaaccagact gacaggtaag tgtagtaatg
    23461 actggcaaca aagaaagcac acccccatgt caccagcgat aacgcttcac gaacaaagcc
    23521 gcggatcagg ctaaccagag aggaaaaagc aatcaccgcg attatggcgt aatcaatcca
    23581 gaccatatgt gtcccacgat tttacgccct gtcatcctgt tcggggcgca ttctaacaga
    23641 aaaagaaaac gtttgcgtag ggatttcctt cccgcgcatc aataaaaatg gcgctgaaaa
    23701 aatattcaac gccatcgact ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc
    23761 ggcgtaaacg ccttatccga cctacggttc tacccctgcg taggcctgat aagacgcacc
    23821 agcgtcgcat caggcaacgg ctgtcgaatg cggcgtgaac gccttatccg acctacggtt
    23881 ctacccctgc gtaggcctga taagacgcac cagcgtcgca tcaggcaacg gctgtcggat
    23941 gcggcgtgaa tgccttatcc gacctacggt tctacccctg cgtaggcctg ataagacgcg
    24001 ccagcgtcgc atcaggcaac ggctgtcgga tgcggcgtaa acgccttatc cgacctacgg
    24061 ctctacccct gcgcaggcct gataagacgc gccagcgtcg cataaggcaa gaccgtatta
    24121 attcggcgtg tagcccatta ccacgccact taagccagaa agttgcttca actcacccag
    24181 cgatcccttc agcttatctt tcgaggcatc tggcccaacc agaatacggg taattttacc
    24241 ctgcactggc gtggatggcg acgtataaac ccgataaccg gcaccgcgca gcttaccgac
    24301 aatctcattc actttatcgg catttttcag cgcgcccagt tgcacaacat aggctttacc
    24361 cgttggcgct gctttttctt ccacgaccgg cttcggttct ggcttcggcg caggtggtgc
    24421 ttcaaccttc ggcttaggcg gctccaccgg tttcggcttc ggtgggacga ccggtgcagg
    24481 ttccggttca aactcggtgt tattggctgc aatagtggtc ggatcgagcg acggtgctgc
    24541 ggcatcacct gcccgcacct cttccgctgc gccttccggc ggctgcgtcg gcaacgcctg
    24601 ggtggcggct ggcatcatat caggctcatc gcgatcgccc gcttttggaa ccagcgggat
    24661 agccgcaaac tcatcctgat aatgtttttt ctgcccgtcc agcagccctg gaagtacaat
    24721 cacccccagc gccaccagca cgatcgtgcc cactaaccga ttctgaaact tacttgccac
    24781 cgcttctcct cgcgtcaatc acttccatga catgtgcgac cgtgtggaac gaaccacaca
    24841 ccagcacggt gtcttccgct ttagcgtccg ccattgcggc atcccatgcc tgcgcaacgc
    24901 tatcaaatga tttgccgtta cccaaatgct caagcagttg ttctgccgtg gcaccgcgcg
    24961 gcccttccag tggcgcacaa taccagtcat caaccacgct tttcaaccag gccagagttc
    25021 cggcaatatc tttatcatgt agcataccga taaccgccag cacgcgcccg tttttcggta
    25081 gcgttttcat acgcccggtg agatattccg ccgcatgtgg attatgcgcg acatcaaaaa
    25141 taacgcgtgg cgactcgctc acaatctgga aacgtcccgg caaaattgcg ctggcaatcc
    25201 cgtcgcgaat ggcattttca ctgacctcca gcccgctggc acgcagtgcc gccagcgccg
    25261 ttgcggcatt cggttgcggg acaagcggca acggcaggtt ttccagcgtg ccgtgagcat
    25321 cgctaaacgc ccagtcatga tcggtgacgg aatagttcca ctcaacgccc cgacgttgta
    25381 acagtgcacc tttttcctgc gccacatcag caatggtaga aggcatttcc ggctcaccga
    25441 caattgccgg tttttcgctg cgaaagatgc ctgctttctc gcgaccaata ctttcgcgat
    25501 ctggccccag ccagtcggta tgatccagcg caatactggt tactaccgcg acatcggcgt
    25561 cgacaatatt ggttgcgtcc agacgaccgc ccagccctac ttccagaatc accacgtcaa
    25621 gttgtgcctg tttgaacagc cacaacgccg acagcgtacc gtactcgaaa taggtcaggg
    25681 aaatatcacc gcgtgccgat tcaatctccg caaaagaggc ggtgtgggcc gattccggca
    25741 attcctgacc ctgcacacgt acgcgctcgg tataacgcac cagatgaggc gaactgtaga
    25801 cgcccacttt gtaccctgcc gccatcagaa tcgactccag cgtacggcag gtggtgcctt
    25861 tgccattcgt acccgcaacg gtaaacacaa atggcgctgg tttcagaacg ccaagacgcg
    25921 ccgcgacctg gctcacgcgc tcaaggccga gatcgatagt tttactgtgc aggttttcca
    25981 gataagaaag ccacgaagcc agaggcgacg cggcttgagg agtgcgtttg ataatcatgg
    26041 tatccgctga ttcgttacgg tgagaatagc aaaagggcag agccagtggc cctgccctta
    26101 tcagttatca ggcctcaggt tcctgatccg gtaccggggg taccactacg ccttcacgcg
    26161 gcgcttcagg attcggcgct ggcagattca tcaacttcgc cagaatgctc gccagtttca
    26221 ggcgcatttc cggacgacgg acgatcatgt cgatcgcgcc tttctcgatc aggaattcac
    26281 tgcgctggaa tcctggcggc agtttttcgc gaacggtctg ttcgataaca cgcggaccgg
    26341 caaagccgat taacgctttc ggttcagcga tgttgagatc gcccagcatg gcgaaacttg
    26401 cagaaacacc gcccatcgtc gggtcggtca gcacggagat gtacggcaag ccgcgctcct
    26461 gcatttttgc cagtgccgca gaggttttcg ccatctgcat cagcgacatc agtgcttcct
    26521 gcatacgtgc gccaccagag gcggagaagc agatcagcgg gcagttatct tccagcgcct
    26581 gctcaacggc acgcacgaaa cgcgcaccca caacagaccc cattgaaccg cccataaagg
    26641 cgaactcgaa tgccgcagcg acaaccggca ttccatacag agtgcctttc atcaccacca
    26701 gcgcatcttt ttcgccagtt tctttctgcg cagatgccag acggtcttta tacttcttgg
    26761 agtcacgaaa cttcagcaca tctttcggct caagctcgct acccagctcc acaaggcttc
    26821 cttcatctaa caggctatgc aggcgattac gcgctgtcat acgcatgtga tggtcacact
    26881 tcggacagac ctcaagatta cgttccagct cagcgcggta taaaacctga ccgcagctat
    26941 cacacttagt ccacacccct tcaggaatgc tcgccttgcg ggtgggagta atgttgcttt
    27001 taattcgttc aatccagctc attagggacc tttctgtctg aacctggttc gatgccagtt
    27061 ttatctttgg ggacgcataa tgccattttt gcccccaaca gaccatgaat gttgcacatt
    27121 aaaacataac agcccgaaac tttggataaa aaagtggtcg aaccgcggag ttacttttta
    27181 ttttgcggcg cgtgccgcag cgcgtttgtg acggattatt tcgatgacgc ccggcaaaat
    27241 agaaaccaca ataatcccga cgatcagcag cttaaggtta tcctgaacca tcgggattgt
    27301 accgaagaaa tagcctgcgt aggtaaaaag cagtacccac aacagtgcgc cgatcacgtt
    27361 ataagcggcg aaatgacggt acgacatgtg gcccattccc gcaacaaacg gggcgaacgt
    27421 tctgacgatc ggcacaaaac gggcgagaat aatcgttttg ccgccatgtt tctcataaaa
    27481 ctgatgggtt ttgtcgagat aactgcgccg gaaaattttc gaattgggat tactgaataa
    27541 cttttcaccg aacaaccgcc cgatagtgta attgaccgcg tcacccacaa tcgcggcgat
    27601 cagcatcagc accaccatca tatggacgtt tagatcgttg gtttccagtg atgccaacgc
    27661 tccggcaaca aacagcaatg aatcacccgg taaaaacggc gttaccacca gaccggtttc
    27721 acagaacaaa attaagaaca aaatggcata aacccagacg ccgtactccg cgaccagctc
    27781 tgccagatgc acgtcaatgt gcaggataaa atcgatgagg aaataaatca ggtccatatt
    27841 tgcctatgcc ttgtactcgt catctttcag gctgtaacta cgttggctac agcctgaaat
    27901 gttccgagca ttattctcgt tagtccgcca gaaatagcgg gcccattggc ggttttggaa
    27961 gatcataccg gtcagggtaa tccaccgcga ccagatacag cccttccgct tttgccgttg
    28021 ctgccgccag cgttctgtcc tttgccgcca gcaactctgc tatccagctc tccggctggt
    28081 tgtgggcacc gacttccatc aggctgccga caatattcct gaccatatga tgtacaaagg
    28141 cattcgcttt gatatcaacc accacataag gaccgtgacg cgtgacatta atatgcataa
    28201 cgttgcgcca cggggttcgg gactggcact gcaccgcacg gaacgaggtg aaatcattct
    28261 cgcccagcaa gcattgcgca gcccgatgca tccgttcagc gtccagcggt tcgtaaaaat
    28321 gggttacccc tttactcagt accgccgggc gcagccgatg attgtagatg atatagcgat
    28381 aacggcgagc cgtggcgcta aatcgggcat gaaaatcatc aggtacagct ttaacccaac
    28441 gcacggcgat gtcaccaggt aaattcgcat ttacgcccaa cgtccacgca gcgtctttgc
    28501 gctgcgcggt tgtttcgaaa tgcacaacct gcccggtacc gtgtacccct gcgtcagtac
    28561 gcccggcgca gaagacggtg atgggttcgt tcgccacctg ggagagcgcc ttttccagct
    28621 tctcctgcac actgcgaact tcgttctgcc gttgccagcc gtaatactta ctgccgtcgt
    28681 actcaatgcc cagcgcaatt ttataaactg gcggttgttg ctggtcggac attagtacag
    28741 atactcctgc accagtttct cggcgatttt tactgccatc agcgcgccgc caaagcgaac
    28801 gttatcggcc accgaccaga actggacttg ctccggcata ccgtagtcat tacgcacgca
    28861 gccaacagaa agatgcggcg taccagaagc atcacctacc tgagttggga attcgttctc
    28921 ttcagagagc acaatatctt ccccttgaac aaacgcatca cgcgcttctt ctgctgccag
    28981 tggacgcaga gcttcaaagt tgaccatctg ggcatgaccg tagaataccg gtgcctggac
    29041 gacgcttgcc gaaatcatca gtccttcgtc ctgcaggatt ttgcgtactt cgtcaacgat
    29101 acgacgttct tcacgcacgc taccttcgct atccggcagt aacggcagca tgttgaacgc
    29161 cagctgacgc ccgaagaaat cttcttcgtc aatcggaatg ccgttgagca atttagcact
    29221 ctgccccgct aacgcatcga ccgctttttt gccctgggcg gaggctgaaa tcaggctggt
    29281 aacgctgata cgtgataaac cgccctgatc gattaacggt ttcagtgccg ccagcaactg
    29341 gctggtcaga ctgtccggta cggcgatgac attccggttg cggtaatcgg tcagtacaaa
    29401 cgggtttact tccggcacca ccagcggtac gtcgggttcg agagcaaaca atccgctgct
    29461 gtcgatcacc aggcaacctg agttggtcgc ttcttcaacc caggcagcgg tagcttcttt
    29521 gcctgcgaca aaaaatgcca gctgcgcctg cgtccagtcg aattcagcgg catcctgcac
    29581 ggtgattgtc ttaccaccaa agcgcagttg ttcgcctgcg ctttcgttac gtgccagtgc
    29641 ataaatttcc ccaaccggga actgacgttc agccagcgtt tcaagcaggg cttcgcccac
    29701 agcgccagtt gcgcccagga cggcaatgtt ccagccttca gacatggtgg tttactccag
    29761 aaatagcaaa gctccctgcc aatgttacag cagagagcat gaagaagaga ttaacgtgcc
    29821 ggatgatgaa cggcgttaaa acccagttta cacagcaatg atgccgcact ggcgtcatca
    29881 caaattacat acagagatga ccattcacgg cgctcaagat agtttttgcg cagtttatcg
    29941 aactcacccg gtatcccggc gactttacgc agcggtgcgt catcgcggcg cacatcatac
    30001 accaaatgca ccagcctttt cagcgtcggt tgatcgagcg ggccatgcag cgtaatgcga
    30061 ccaaactctg gcgcgggcag taatgtatcc agcgcaacgt gctgttcatg cccaataaac
    30121 ttgctatagg cttcaaacac ttgcgtagta ccgcgtgctt taccttccag ggtatagcct
    30181 gcaatatgcg gcgtgccgat atccactttt gtcagcaatt ccacattaag ttccggctcg
    30241 ccttcccaga catccagcac cacgcttaac ttctggcctt cattcaggca ggtcagcagc
    30301 gcggtattat cgacgactgc gccacggcag gcgttaatca gaatcgctcc cggtttcagg
    30361 ctacggatca gtttttcatc cgccagatgc agcgttttgt actgcccgtc tttgaagagt
    30421 ggcgtatgga aagtcagaat atccgcatgc tgaactaatt catccagcga gcggaaatca
    30481 ccctcatccc cacggtcggc gcgaggcgga tcgcaaagta aggttttaat ccctaacgct
    30541 tccagtcgtg cctgtaaacg ccgaccaacg ttaccaacgc ccacgatccc cacggtacgc
    30601 tcgtgcagtg aaaatccatc gcgttcggca agcatcagca gggaggagaa aacatattcc
    30661 accaccgcaa tcgcattaca gccaggtgca gcggaaaaac caattcccgc ctgcttcaac
    30721 catgcttcat cgacatggtc ggtccccgca gtggcagtgc caacaaattt aatgggtttt
    30781 cctgccagca aagattcatt cactttcgtg accgaacgca ccatcagcgc atccgcgtct
    30841 gccagttgag cgacggggat tggacgcccg ggaaccgcga tcacctcacc caaacggcta
    30901 aataagtcgc gggcataagg catattttca tcaacaagga ttttcacgtt tgtgttacct
    30961 gtatgagacg agagttaacc ggacaagtgt gccataatct cgcggccagg catacttgcg
    31021 aagatttcag gtataaggat acgtaatgat acaacctatt tccggccctc ctcctgggca
    31081 accaccaggt cagggagata acctgccgtc tggcgcgggc aatcagcctt tatccagtca
    31141 gcaacgtact tcgctggaaa gcttaatgac gaaagtgacc tcactgacgc aacagcaaag
    31201 agcagaactg tgggcgggta tcaggcacga tattggtctg tcgggagatt caccgctgct
    31261 ttcgcgtcac ttccctgccg ctgagcataa tctggcgcaa cgtctgctgg ccgcgcaaaa
    31321 aagccattct gcccgccagc ttttagcgca attaggggag tatttacgtc tggggaataa
    31381 tcgtcaggcg gtcacggatt atatccgtca taactttggt cagacgccgc tgaatcagct
    31441 ctcaccggag caattaaaaa ccattctcac cctgttgcag gaagggaaga tggttattcc
    31501 gcaaccgcag cagcgcgagg cgaccgatcg tcctttatta ccggcggagc acaatgcgct
    31561 caaacagctg gtgaccaaac ttgcggcggc aacgggggaa cccagtaaac agatctggca
    31621 atcgatgctg gaactttccg gggtgaaaga tggcgagtta attccagcga aactgtttaa
    31681 ccatctggtg acctggctgc aggcgcgtca gacgctaagc cagcaaaata cgccgacgct
    31741 ggaatcacta cagatgacgc taaaacaacc tttagatgcc agtgaactgg cggcgttatc
    31801 ggcatatatc cagcaaaaat atggcctttc tgcgcaatca acgctttctt ctgcccaggc
    31861 cgaggatatt cttaatcagc tttatcaacg gcgggttaaa ggaattgatc cgcgtgatat
    31921 gcaaccgctg cttaatcctt ttccaccgat gatggacacg ttgcaaaata tggcaacgcg
    31981 tcccgcgctg tggatactgt tagtcgcgat tatcctgatg ctggtctggc tggttcgtta
    32041 accccgacga aatgacagta tcgtgacaat aattcccagc accgcagaga tcgccccggc
    32101 aagaaatacc gaagagtaac caaacgtggt cgccagcatt cccgccagcg gcccggagac
    32161 gccgagggcg atatcctgaa acgcggcgta accgcccagt gcggtgccgc gaacttgtga
    32221 ggggacacgt ttaaccacct ccacgcccag cgcaggaaag ataagcgaac atccggctcc
    32281 ggttaacgcc gcgcccgcta atgcgaccca cgcacccggg gcttgccaga gcagcaacaa
    32341 gcccaccgtt tctacaagca gagagacgat cgccactttc acgccgccaa aacgatccgg
    32401 catccagcca aacatgacgc gcatcacgac aaatgcgccg ccaaacgcgg taagagtaaa
    32461 gcccgccatc gcccatcctt tgctggcaaa gtagagcgaa acgaaagtcc cgataaccgc
    32521 aaaaccaacg ccttgtagtg ccagccctaa ccctggtttc cagataagcc cgacaacgct
    32581 ccacagcgat ggacgttctc ccgccagggc cggtactttg cgcactgtgc cgttacaggc
    32641 ccacgccagt aagggtaata ccattgtggt gatcgccagt gcggcaaaac cgtaatggct
    32701 atgaatcaac aggccaagcg gagcaccaac agcgagggca ccgtaaatcg ccattccatt
    32761 ccatgacatc actttgccag agtgttttgg ccctacgatg cctaaccccc aggtcagagc
    32821 gcctgtcagt aactggcttt caccaaagcc aagaatcaaa cgcccgatga ccaacagggc
    32881 aaatttgaac ggtgcggaga caggcaaaat cgccgccagc agcaacgcgc cgccagccag
    32941 accacaagct aacatcccct gaagcgccga acgttttgcg ccatattgat cggccagtcg
    33001 cccggcatag ccacgcgtca gcaccgtagc cagaaactga atcccgacgg caatgccgac
    33061 catggtattg ccatagccca gttcatgatg aacaaacagc gggataaccg gcaacggcag
    33121 ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg cggaagagcg aaaaattggc
    33181 agaagatcgt gtttcggttt ggcttacagc agtcatgcat tactccagaa tgcagcgcaa
    33241 ggcgaggagt atccccgtct catctctctg gtttcagggt tacggtgcgt tggcaggaat
    33301 taacgcgtac gtcttttcag aaggaaatcg acaaagcggg aagtttgcct ggaacaggcg
    33361 gcgattgtca atgatgtgaa aaagggaacc atcgggttcc ctttggcgtt agtgccggag
    33421 gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt cttatccgac ctactccggc
    33481 ctactgcgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttggtg ccgccgaagc
    33541 cgaagctgtt agacataaca gtggtcagtt cgcgatcggt cgtttcggtc acgatgttca
    33601 gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata aagccgtgtt
    33661 ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccaga gagtgaccgg
    33721 tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca cggatagctg
    33781 ccagctcttt cacgtcgcca accggagtcg aagtgccgtg ggagttcagg taatcgattg
    33841 gggtatcaac gccatgcatc gccatctgca tacaacgtac tgcgccttcg ccagacggag
    33901 caaccatgtc tgcgccatca gacgttgcgc cgtagccaac gatttcagca tagatgtgag
    33961 caccacgcgc cagcgcgtgt tccagctctt caaccaccac cataccgccg ccgccagcga
    34021 taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg gtgtcgttgt
    34081 atttagtaga cagcgcaccc attgcgtcga attcgcaagc catttcccag cacagctctt
    34141 cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct actgcgttac
    34201 cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg ccatgaattt
    34261 taaacggcgt ggcgagacag gcagaaacgc cggatgccat cgctttggtg accacatacg
    34321 ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac acctggaaac
    34381 gcggagagcc gccgccggaa cctgcaatca ggccaacgcg cgggttattc tggtaagctt
    34441 ccggagagag gcccgcatca gcgattgcct gctccataga aaggaatgca taaatggatg
    34501 cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc agttttacgt
    34561 tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa gtgatccctg
    34621 aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg ctggaaacaa
    34681 tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg cacatagagt
    34741 aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc agccaattaa
    34801 tagagaaatt tgcgcagcct tatacacatc gctaagatcg agccaccgcc tgtaagacga
    34861 gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa tgctgagggt
    34921 acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg gctggaagag
    34981 acgcgttatg tttttctcgg aggtaaccaa ttagaggcac gctttcctga gcatccacat
    35041 cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct gacgctatgg
    35101 caggcatttg atcagtttcg cgaagcgcat ccgcaagcgc aattacaacg cttacatttc
    35161 attagttttg agaaatttcc cctcacccgt tcggatttag ccttagcgca tcaacactgg
    35221 ccggaactgg ctccgtgggc agaacaactt caggcgcagt ggccaatgcc cttgcccggt
    35281 tgccatcgtt tattactcga tgaaggccgc gtgacgctgg atttatggtt tggcgatatt
    35341 aatgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc ctggtttctg
    35401 gacggctttg cgccagcgaa aaacccggat atgtggacgc aaaatctgtt taatgccatg
    35461 gcaaggttgg cgcgtccagg cggcacgctg gcgacattta cgtctgccgg ttttgtccgc
    35521 cgcggtttgc aggacgccgg attcacgatg caaaaacgta agggctttgg gcgcaaacgg
    35581 gaaatgcttt gtggggtgat ggaacagaca ttatcactcc cctgctccac gccgtggttt
    35641 aaccgtacgg gcagcagcaa acgggaagtt gcgattatcg gcggcggtat tgccagcgca
    35701 ttgttgtcgc tggcgttatt acggcgcggc tggcaagtaa cgctttattg cgcggatgaa
    35761 gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc tgtatccgct cttaagtaaa
    35821 cacgatgagg cgttgaatcg ctttttctct aatgcgttta cttttgctcg tcggttttac
    35881 gaccaattac ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt aggctgggat
    35941 gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg atttacccgc agaactggct
    36001 gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa ttgcagcggc
    36061 attacttatc cgcaaggtgg ttggctgtgc cctgcagaac tgacccgtaa tgtgctggaa
    36121 ctggcgcaac agcagggttt gcagatttat tatcaatatc agttacagga tttatcccgt
    36181 aaggatgact gttggttgtt gaattttgca ggagatcagc aagcgacgca tagtgtggtg
    36241 gtactggcga acgggcatca aatcagccgt ttcagccaaa catcatctct gccagtatat
    36301 tcggttgccg gtcaggtcag ccatattccg acgacaccgg aactggcaaa actgaagcag
    36361 gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcagca tcattgtatt
    36421 ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca gtgatgaaga tcagcagcag
    36481 aatcgccagc ggttgattga ttgtttcccg caggcacagt gggcaaaaga ggttgatatc
    36541 agtgagaaag aggcgcgctg cggtgtgcgt tgtgccaccc gcgatcatct gccaatggta
    36601 ggtaatgttc ccgattatga ggcaacactc gtggaatatg cgtcgctggc ggagcagaaa
    36661 gataaggcgg taagcgcgcc ggtttatgac gatctcttta tgcttgctgc tttgggttcg
    36721 cgtggtttgt gttctgcccc gctgtgtgcc gagattctgg cggcgcagat gagcgacgaa
    36781 ccgattccga tggatgccag tacgctggcg gcgttaaatc cgaatcggtt atgggtgcgg
    36841 aaattgttga agggtaaagc agttaaggct gggtaaccag ctttggcatt gtttgtcgga
    36901 tgcggcgtga acgccttatc cgacctacgt gtgacgtgta ggcctgataa gacgcgataa
    36961 gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac tgctgtgatg cctgctgaaa
    37021 caggttttcc cacatatcgg tcaccagcgc ctggtcacgc ggcgacaatt caccagcacc
    37081 aatggctttt tccagactct ggctaacggt cgtatgcacc gcctgagcgg agtggtcatc
    37141 accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc cactggcaaa
    37201 caactcatca tcactggcat ggtcaaccat accgtcgatt aatgccagaa tgcgtgattc
    37261 aaactccgcg atcatcttct ttcctcattg taaaacgtgg cgctggcttc agttgagcgc
    37321 ttccggccac gggaactgtt ccgccgtaat ttccggcgtg tgataataat tttgtagtgc
    37381 tttaatgaaa cgtgccgggc gtgccggaat gccatttgtt agataatcca tcacctgcgc
    37441 atgtactcgc cgttgaaaca ccacgcggtc cggttcgaag tcgccttcca gattgtcgca
    37501 actgacgtta aacggatatc cagccgccac gcagaacaac cagtcgagcg cctgcggctt
    37561 cacttcaaca tcttcaaact ggctttgcgt tcgggcatcg cgtccgtccg ggcaatacca
    37621 gtagccgaaa tcaaccagct cacggcgcgc ttttccggca atacaccagt gcgaaatctc
    37681 atgaattgcg ctggcataaa agccatgagc aaagacgatt cggttatacg gtacttccgc
    37741 atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat taaaatcatc
    37801 ggcaaagcag ctattaaaaa tttcaatcaa ctgctcgtag tggtgtgtac tgttcattag
    37861 ttcatcccca accagtggag gatctcctgt ccatggctat cgtaaagtaa tttggcactc
    37921 atcaccgccg agacgataac gatcatcggg cggatcagtt tttgtccttt gctcaacacc
    37981 agtcttgaac ccatgcgcgc gccgaggaac tgccccacta gcatcacaaa gcccgtcgcc
    38041 caaatcactt tgccgccgag aataaacagc agtaaaccac cgatgtttga cgttgcgttg
    38101 agtaatttgg cgtgagccgt ggctttggcg aggttgaatc cgcacagcgt aacaaaggcc
    38161 agcgcgtaaa acgatccggc tgccggacca aagaatccgt cataaaaacc gacgcagcca
    38221 ccggcaatta acgcgaaggg taaaccgtac atccggcgct ggcgatcttc ttcacccagc
    38281 tttggcatca gcaagaaata gaggccgata caaatcacca gaatgggcaa aatctgccgt
    38341 aagacatcag cctgaacgta ttgcaccagc agtgcgccgc tcattgagcc gataaaggtc
    38401 atggcgatat tgagtttctg atcacttaaa ctaaccactt tgcggcgaat aaagtagata
    38461 gtggcggaaa tagagccgcc gcaggcttgc agtttattgg ttgccagcgc attagcggga
    38521 gacatccccg ctgccatcaa tgccggaatg gtgagtaacc caccgccacc ggcaatcgag
    38581 tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcaacag cggggaaacc
    38641 ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc gcctggcagg
    38701 aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt tttggaggtt
    38761 caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa ggttgatctt
    38821 cacactccag actatcggca gggcaacgta atcgtacatg catatgcgcg cgatgctgga
    38881 accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg caaagttgtt
    38941 gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg gcgagtttga
    39001 tcaagctgaa aatttccggc ttccacagcg tggagacaac gtgtttaccg tcgcgggaaa
    39061 ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt ccagcgagtt ttcggcagtt
    39121 gcaggaagat atcaacatcc agtccggtct ggtggctggc atgaccgccg ttgaaacgcc
    39181 caccagcggg catccccata tcgccaatca gcaccgtacc catgcccaga ttgctcacct
    39241 ggctactcag acgctggata aacatcacca ggtccgggtg accgaaatag cgacgctgat
    39301 cggtacgcat gacctgataa tgttcggact gtatcggcag cgtgtcagcg ccgacgatac
    39361 agccattaga aaaactgcct atcgattgtg cgctacctgg cacaggttgg gttatttttt
    39421 gccacggcgc cgctgccagg ctgacgctac tggcaagcag ggccagcagc gcaatcgcgg
    39481 ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt tgcgcccgct
    39541 gccgtagcag gtgatccatt aaaacgatcg ccagcatcgc ttcagcgatc ggcaccgcgc
    39601 ggatcccgac acagggatcg tgacggcctt tggtgatcat ctcaacttct tcgccaaagc
    39661 ggttaatggt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc atatgggcaa
    39721 tgatttgctg cccgctgctg ataccgccga gaatgccgcc cgcatggttg ctctggaaac
    39781 cgtctttggt gatttcgtcg cgattctggc tgccgcgcag cgccactacg tcaaaaccgt
    39841 cgccaatttc cacgcctttc accgcgttga tgctcatcag cgcatgggcg atgtcggcat
    39901 ccaggcggtc aaagaccggc tcgccaagtc cggcggggac accactggct acaacggtga
    39961 ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg catcagttca tctaacgcgt
    40021 cgattttgtc cgggtccggg cagaagaacg gattttgttc aacctgcgac cagtctttga
    40081 tttccagcgg aatgtcgccc atctgggtca ggcagccgcg gatttcaata ccaaatgtct
    40141 cggcgagata ttttttggca atcgcccctg ccgccacgcg catggcggtt tcgcgggcgg
    40201 aagaacgtcc accgccgcga taatcgcgca gaccgtattt ttgttcgtag gtgtaatcgg
    40261 catggccagg acggaaaacg tccttaatcg cactataatc ctgagaacgc tgatcggtat
    40321 tttcgatcaa caaaccaatg ctggtgccgg tagtaacacc ttcaaaaaca ccggagagaa
    40381 ttttgacctg atccggctca cgacgctggg tggtatagcg cgatgtccca gggcgacgac
    40441 gatcaaggtc atgttgcagg tccgcttccg tcagcggaat gcctggcgga acaccatcga
    40501 cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt ggttacgcga aagagttgtc
    40561 caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt tacttaatct
    40621 ttataaatcg cgaaatgttc acgggccgcc agcagctgct ctttggtgag cataaacaca
    40681 ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata ttgttccata
    40741 agatgtacca tgctgttgcc gacttcacaa atcagcacac catcatcagc aaggtaatct
    40801 gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt cagtgccaga tgccaggccc
    40861 agttccggct cgtgacggta ttcgtttggc aggtcggaca tatcttccgc atcgacatac
    40921 ggcgggttag tgacgatcag gtcgtactgc actttcggca aatcacggaa cagatcggaa
    40981 cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc ggcaaccgcc
    41041 agcgcgtcgg gagagatatc caccgcgtcg acttccgcat ccgggaaggc ataagcacag
    41101 gcaatggcga tgcagccgct accggtacac atatctaaaa tatgctgcgg ttgcttgctg
    41161 ataagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg caccagcaca
    41221 cgttcatcga cgtaaaattc atggccgcag aaccacgctt tgttggtcag gtaagcaacc
    41281 ggaatgcgtt cgttgacgcg gcggatcacg cgttcaacaa tacggtgttt ttcgctggag
    41341 gtcagacgcg cggtgcgcat atcttccgga atatccagcg gcaggtagag cgacggcaat
    41401 accagctgca cggcttcatc ccacgggtta tcggtgccgt gaccgtacca gatatttgcc
    41461 gcgctgaagc ggcttaccga ccagcgcaac atgtcctgaa tggtttgcag ctcatttact
    41521 gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca ataatttcgg
    41581 cggctagttt gccatgaaga tgacgataaa tcagcattca cgcgcggtga gtgaggaaaa
    41641 atacgtttaa aacgatcgat tgcgctacga gtcgggtaaa ctgtaggaaa attagaaata
    41701 gagacagata aatgaaaaag aaaacaacac ttagcgagga ggaccaggct ctgtttcgcc
    41761 agttgatggc ggggactcgc aagattaagc aggacacgat tgtccaccga ccgcagcgta
    41821 agaaaatcag cgaagtgccg gtgaaacgct tgatccagga gcaggctgat gccagccatt
    41881 atttctccga tgagtttcag ccgttattaa ataccgaagg tccggttaaa tatgttcgcc
    41941 cggatgtgag tcattttgag gcgaagaagt tgcgacgcgg tgattattcg ccggaattgt
    42001 ttttggattt acacggtctg acgcagctgc aggccaagca ggaactgggc gcgttgattg
    42061 ccgcctgccg ccgtgaacat gtgttttgcg cttgcgtgat gcatgggcat ggcaagcata
    42121 ttttgaaaca gcaaacgccg ctgtggctgg cgcagcatcc gcatgtgatg gcatttcacc
    42181 aggcaccgaa agagtacggc ggagatgcag cgttgctggt gttgattgaa gtggaagagt
    42241 ggttgccgcc ggagttgccg tgaatattaa gtgccggatg tggcactggc accgaatgcc
    42301 ggatgcggcg tgaacgcctt atccggccta caaagtgtgc aaattcaata cgttgtcatt
    42361 catttgtagg cctgataaga cgcgtcagcg tcgcatcagg catcagcaca caaccgccgg
    42421 atgcggcgtg ctagaacata ttcaacacat actgaaagcg acacgttgaa tcaataggct
    42481 gcaatgcgct ggtatctacg cgcaccacaa tatcatgcag ttgcctgtca gtaatcggat
    42541 gcggaaccgg cgtcaccgtc acttttgcat cgtcgcagcc tgatgcggta aaggtgatca
    42601 tctgcgaaac aaaacggcaa ctagccccgc gatcggcaaa caggctacca tcacacaact
    42661 tctgactttc cattaaatag gttgacaggg ttgtctcaat gatcccccct gtggattgca
    42721 gatttaatgt ttttttatga gccaccgtat tctcatccca ggaaacatca tacgtcgtca
    42781 gcggaattga cgggcaggtg gagttatgga ctttagggcc gacataggta gcccctacac
    42841 tccccaggtc cgtagtggta atctggcttg acactaacca cggacacccc tgcgcatccc
    42901 cggttacagt gacttctaat tcagccataa tacactccca actaccgtcg gtacctggta
    42961 cactggacag gccagggcaa ttagacatag atgtattatc tgaatggttc aaataaatgg
    43021 ttggtgcagg tatggaagac ggaccattcc agctaagcaa ggttaagttg ccaaccacaa
    43081 gcagaggcgg tttactcgat tgatatgtac ctgtatatac ccccgatgca gtaacaacct
    43141 ccagagaaaa cacaatcctg gaaaaagcgc ttggataacc cataggggtt tccatatgaa
    43201 acgactcagc ctgagccgtc aggctcaata ccatcagcgt caacaacccc ataaaatttc
    43261 gtagtagttt catcttaaat gtactcaaaa gtggcagtca ggctggcggt aaagtcacca
    43321 gaagttacgt cacgaccgct tttcgcctgc aaccaggcat taaaattgag gttattcgca
    43381 ccgttactga ggttaaaggt ggcaccggca gagtcgttaa gcggcaccca ggtaccgtcg
    43441 gacttttcca taccaatccc taccccctgc gcccccgaag ccgggtcaag cgccagaaat
    43501 cctggctgtt cgctgtcttc cgtaccggtc agcgtgactt tgacctggta atccgccggg
    43561 cctttgcaat ctttcaactt aaagaccacc ggcaccctgg aagactgccc cgcaaccatt
    43621 aaatcctgac tgctaacggt ggggaaatgc acctctgcca gcagttcgcc atccaccacc
    43681 agggtgcagg atttactcaa taaattgccg taaaaatgca gattgtccgc cgccaatgtg
    43741 gttccgctgg cggcgagcag caggcctgtc aggagcatac atccggtctt tgttttcatt
    43801 gataatccac ccgtaatgtt gcgtacgctg aaaacgcgcc gtcatttaat gtgacgccgc
    43861 tcgctttaac cggcacagcc tccagcttcg gcttttgtgt caaatccgtg gcgttaatgg
    43921 tcagcggggt attgagctga aaacgctgcc cgttctgcaa aagctgaata ccgaatcccg
    43981 gaacatcggt ttcgatggct gcgtcgttgt agctggtttg cgtgcccgtc caggtgagcg
    44041 tcatctccca ggcctcattg cgaacgttgg agtcacattt gatggtgtac ggcacctcct
    44101 ggcgggcgta gttgccatca atactgtcga taagcacatc gcgaaattcc agctcaatgg
    44161 tccggccttc gttgatggtg caggcaggcg gcgacaccag catgccgtga aaggtgatat
    44221 ccggtgaggc ctgaacgcca ccgcatacac cacaaatcag caaaagtgcg agtcgtttca
    44281 ttggtaatcc accaccatgg tcatactggc gttaaattcc gctggcgcga gttgcacgcc
    44341 gttctgtttt accggcacag ccttcagttg cggcagagag ttaaatgcaa aaggcgtcca
    44401 gttattttca ccgatggaaa acagtgaatt atcggcggcg ttttcgatac ggatgccaaa
    44461 cccggcaatg cctgcatcca gcacagtttc accgttcaca gtggtggtag tgcccttcag
    44521 ttgcatccgt aggtcatctt ccagacgccc cgaacagtcg agggtgtagc ctgcattctg
    44581 gcggtagttg ttgccatcaa tactggcaat cagcacatcg ccaaactcca ctgcactacc
    44641 ggtaacggta cagggcggtg gcgcatccac cctgaccctg agttccaggt cgacgggtct
    44701 tgtttccgca ctggcaacat tccaggccag cagcgcaata cccgagcctg ccagtaaaat
    44761 tcttctcata tggctctccc ggcatcagcc tttgcggctc tgctctgcgt ccacctggca
    44821 ggtatcgccg ccacatttaa aatacagcgg catccgcgcg ccatagtcgt tgacgtaggt
    44881 cagcaccggg acgctatcca ttttgacatt cagcggcacg gaggtttttg gcgatatcac
    44941 catgggggaa aatcccgctg ccggattgcc atttttctgc gcactggcgt tactgataac
    45001 cacgtagtac ggcgtcgggt tattgacggt aaactcctga ccgctgcggg ttaaggtcac
    45061 cttatgctgc cacggatttt tcatgctgac gttttccagc gcttttggtc gccagaacag
    45121 cttgatgcgc gtttgcagcg caatctgtaa cgtgttgggt ttgttcgatt ttggcggaat
    45181 ttcccgcacg ttgaagtaga acacgctctc gcgatcggca ggcagcttgt tgatatccgg
    45241 catgccctgt accttcacct gaccgttcat catggaatcg atgcgctgca ccggcggcag
    45301 taccgtcagt ggcgagctaa tcttgttacc ctgctcgtct tccatccagc tttgtgccag
    45361 atacggcatt ttcgggtcgt tattgcgcag cgtgacgctg attgatttgt cgctttcatt
    45421 aaaaatcaaa cgggtacggt ctggggtgac agaggcctgg gcttgcaagc tggtggcagt
    45481 tatcagtaac gcaagcgtgg ttgcgaccgt tttggcagaa aatagattcg tcatggatca
    45541 ttccttaatc acgttattaa ttggctgata ccgatactgg cggttccgtt ggcgtgacct
    45601 gctgagtctg ctcctggatc accggcttaa tttcattgtg taccggggaa gatatcgccc
    45661 ctgtttgctg gcatggcaat aacaggccat tgaacagatc ggctggcagc gggtcaggca
    45721 gatgaatgtc gcagtgggcc acaccgcccc atgaaacgat catatgctcg ccaggtttga
    45781 caccggcgag gtagacgttg ccgtcatcgt caaccagacc aacattctgc gcgttgtcgt
    45841 ttttcacttc cgcgccaaac ggcggatggc tgccatcctg cagacgcagt accgccatcg
    45901 ctttttgccc gctgatcacc gagaacttac ggtagccaat ggccccttcg gtgagcgtgg
    45961 cctgcaccac agactgagtg gcttcggcgt tttccggcag gttgttaagg tcgatatacg
    46021 cctggttgcg gtaatagttg ttgacgtctg ccactacggc cttaccgaac atattggtgt
    46081 aaaccgccgc gccatttcct tccaccggaa caccggccac gccatcggca tcaattaaca
    46141 gacgcgtgcc gcccatattc tgggtacggt ggagcgcgcc gccctgcgcg gtgagcgtcg
    46201 ccccgccctg gagtgaaatc cctgccgagg tgtactgccc ttcatggtag ttggcgctga
    46261 gatcgacctg cgcaagcgat ccatcgtggc tgtaatagcc gtcaacgctg gaatgattgt
    46321 cactggttcc cacgttcaac tggtaatgag ttgcgtcatc gacacggctg aaatacccca
    46381 cctgactgct gtcggaaccg ctgccgtagt tgccgttata gctgatggtg ctgctgtcgc
    46441 cccacggcat actgagcgaa atgtacacac ctttatcggt ctggttgtca tactcgtagc
    46501 ggtatccggt catggaaacg ctcatattgc ggatactgcc taaattgaag tagtgcgaga
    46561 gcatgacgtt gtagttggtc tgctcgtcgc gatcccagta ggtatggcgg gtgtagttga
    46621 gatagacaga cacaccggca tccctgaagt tctggttata ggtggcggta tacatctctt
    46681 tgtcgttgcc ggtgcgcacc atttcgctgt cgctggcatc gagatactcg ctcatggtca
    46741 tgaagttctc ttccgagaag cggtatccgg caaaggtgac gcggctgttc agttcatcaa
    46801 agtctttgga atagctcacg cgaaacgagt taccgtccag cgccccttta ccgtaggcgg
    46861 tctctttatc cagacgggta tgcgagtggg tgatatcaaa ggccaccgcg ccaaagacag
    46921 acagatcgcg accgacgcca agcgccgccg actgatagtg ttcatcagcc agcgccccgc
    46981 cgtacagcga ccagccgtta gcaatccccc aggaggcttc gccgccggag aagaaaccgc
    47041 cttctacatg atgcccccac tcctgcgggc ggcccatcat cagtttatag cgcacctggc
    47101 cggggcgggt caggaacggc atcgaggcgg tgttgatgtc atattcctgc acctggccgt
    47161 tctgctcttc aatgcggata tgcaacgtac cggagacgga atcgcccaga tcctggatgc
    47221 ggaaaggccc cgccggaacc tgggtttcgt aaatcacgcg gcccatctgg ctgacggtca
    47281 ctttcgcggt ggtgtgcgcc acgccggaga tatccggcgc atagccgcgc agattcggcg
    47341 gcagcatttg atcgtcggtg ctgatactgc cacccacgta gttaaagccg tcgaaaatat
    47401 cagaattcag gtagtcttcg ccaaggccaa gtttggcttt tagcgacggt aaggcccgcc
    47461 aggcgtagta gcggctccat tcccagtttt tcagcgtgtc atcaccgttg ataacatcgt
    47521 catcattgct tttgctgtgc tggtagtcag tctgccagtc ggcacgaaga cgccatgcgc
    47581 cgaggttcac cccaaccgtc ccgttaccgc taatttcatt gctgtcatcc ccgccatttt
    47641 cttcatgtcg tgtctgggcg gtaatactgt agtcagcaat taaaccagat ataccgtcat
    47701 cccagcgtga aggcggatcc cagttgatgt cggtatattc aaggtaagcc tggggtaatg
    47761 aaatgactaa cgctgactga ctcaggtcag ctttaatatc aatgccttcc agttgaccgg
    47821 gtttcaggca tttgccgtcg tggatccatt gcaggttttt tgccacatcc tcttttaagc
    47881 caaactgcgc gaccagttca ggcgtcaggc aggcataggt tttactggca tcgttctcag
    47941 aggcgtacca gtaaatatcg tattcttccg tcagcggctg tttatttagt tgaacctgta
    48001 aattgtattt cccaggctcg acataaccct ggctggaaaa tcgcttcaga tcgatttttg
    48061 tgtcgccttt taactccaga aaacgggaat caaactgaat ttcatcttca gcccaggcat
    48121 tgacataact cccggacatt gccaatgcaa cgcagcaggg aagtacccga agtcggaaaa
    48181 gagaatggtc aggcatacac gtatccatcc gtaattatta taatttgcgc gaattccatt
    48241 taaaaacccc atagcccgaa agctatgggg gtatcactta gagataagta atctggaagg
    48301 tcgtgttggc ttcaaaatta cctaaatctg gcgcatcagc agcgcccacc agccaggctt
    48361 taaagtccag cgtctgcttc gctttgcctt tgttggtcgc cgtatcgttt acgattttct
    48421 gttcgatacc cgcgccgctt ttataagagg ttccctgagc gtcaccaatg gccaggctga
    48481 cattgttaaa tgccgcacca gtatcggtat tgtaaatggt gtagtaattg ccgctgttgg
    48541 tagaagacgc gttaccggtg aaggtagtgg tcatcgttgt ctgggtgtca aacacacagt
    48601 cttgcagatg gatctggaaa gctttcggtg tagaagtccc gttgtttttc aggatccctg
    48661 aaccaatgtc acccatatca acttccagtt tgtgatcatc aggaacgatg gagcacgggg
    48721 aatcttcaat ggtgccgtag aaacgtgctg taccattgtt gccagcggcg attgttgaag
    48781 tagaagcgaa cgcacccatt accattgttg cagcaatagc tgtttttaca aacttactca
    48841 ttatttgtcc ttaaaccata aataattaaa aatataaaca ttgcaataca ttgattcagt
    48901 caatagcaat tattt
//
LOCUS       DAPGRN010000023        47743 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPGRN010000023 DAPGRN010000000
VERSION     DAPGRN010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.023"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..704
                     /gene="comR"
                     /locus_tag="RHW33_001447"
     CDS             72..704
                     /gene="comR"
                     /locus_tag="RHW33_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV1644970.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTGKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(766..1305)
                     /locus_tag="RHW33_001448"
     CDS             complement(766..1305)
                     /locus_tag="RHW33_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV1644971.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RHW33_001449"
     CDS             complement(1532..2836)
                     /gene="ndh"
                     /locus_tag="RHW33_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV1644972.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3236..3778)
                     /locus_tag="RHW33_001450"
     CDS             complement(3236..3778)
                     /locus_tag="RHW33_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1644973.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3801..4826)
                     /gene="nagZ"
                     /locus_tag="RHW33_001451"
     CDS             complement(3801..4826)
                     /gene="nagZ"
                     /locus_tag="RHW33_001451"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV1644974.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(4837..5661)
                     /gene="thiK"
                     /locus_tag="RHW33_001452"
     CDS             complement(4837..5661)
                     /gene="thiK"
                     /locus_tag="RHW33_001452"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV1644975.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLHDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5642..6283)
                     /gene="lpoB"
                     /locus_tag="RHW33_001453"
     CDS             complement(5642..6283)
                     /gene="lpoB"
                     /locus_tag="RHW33_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV1644976.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6297..6674)
                     /locus_tag="RHW33_001454"
     CDS             complement(6297..6674)
                     /locus_tag="RHW33_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV1644977.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6677..7036)
                     /gene="hinT"
                     /locus_tag="RHW33_001455"
     CDS             complement(6677..7036)
                     /gene="hinT"
                     /locus_tag="RHW33_001455"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV1644978.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7370..9559
                     /gene="fhuE"
                     /locus_tag="RHW33_001456"
     CDS             7370..9559
                     /gene="fhuE"
                     /locus_tag="RHW33_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV1644979.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9619..11052)
                     /gene="ptsG"
                     /locus_tag="RHW33_001457"
     CDS             complement(9619..11052)
                     /gene="ptsG"
                     /locus_tag="RHW33_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV1644980.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11347..12144)
                     /locus_tag="RHW33_001458"
     CDS             complement(11347..12144)
                     /locus_tag="RHW33_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1644981.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12155..13159)
                     /gene="holB"
                     /locus_tag="RHW33_001459"
     CDS             complement(12155..13159)
                     /gene="holB"
                     /locus_tag="RHW33_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV1644982.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGVDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13156..13797)
                     /locus_tag="RHW33_001460"
     CDS             complement(13156..13797)
                     /locus_tag="RHW33_001460"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV1644983.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13787..14809)
                     /gene="yceG"
                     /locus_tag="RHW33_001461"
     CDS             complement(13787..14809)
                     /gene="yceG"
                     /locus_tag="RHW33_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV1644984.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRYLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14812..15621)
                     /gene="pabC"
                     /locus_tag="RHW33_001462"
     CDS             complement(14812..15621)
                     /gene="pabC"
                     /locus_tag="RHW33_001462"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV1644985.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15741..16982)
                     /gene="fabF"
                     /locus_tag="RHW33_001463"
     CDS             complement(15741..16982)
                     /gene="fabF"
                     /locus_tag="RHW33_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV1644986.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17070..17306)
                     /gene="acpP"
                     /locus_tag="RHW33_001464"
     CDS             complement(17070..17306)
                     /gene="acpP"
                     /locus_tag="RHW33_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV1644987.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17517..18251)
                     /gene="fabG"
                     /locus_tag="RHW33_001465"
     CDS             complement(17517..18251)
                     /gene="fabG"
                     /locus_tag="RHW33_001465"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV1644988.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18264..19193)
                     /gene="fabD"
                     /locus_tag="RHW33_001466"
     CDS             complement(18264..19193)
                     /gene="fabD"
                     /locus_tag="RHW33_001466"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV1644989.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPALMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19209..20162)
                     /gene="fabH"
                     /locus_tag="RHW33_001467"
     CDS             complement(19209..20162)
                     /gene="fabH"
                     /locus_tag="RHW33_001467"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV1644990.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLEANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20230..21300)
                     /gene="plsX"
                     /locus_tag="RHW33_001468"
     CDS             complement(20230..21300)
                     /gene="plsX"
                     /locus_tag="RHW33_001468"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV1644991.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21381..21554)
                     /gene="rpmF"
                     /locus_tag="RHW33_001469"
     CDS             complement(21381..21554)
                     /gene="rpmF"
                     /locus_tag="RHW33_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV1644992.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21606..22127)
                     /gene="yceD"
                     /locus_tag="RHW33_001470"
     CDS             complement(21606..22127)
                     /gene="yceD"
                     /locus_tag="RHW33_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV1644993.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22326..22910
                     /gene="yceF"
                     /locus_tag="RHW33_001471"
     CDS             22326..22910
                     /gene="yceF"
                     /locus_tag="RHW33_001471"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV1644994.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPHSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23022..23981)
                     /gene="rluC"
                     /locus_tag="RHW33_001472"
     CDS             complement(23022..23981)
                     /gene="rluC"
                     /locus_tag="RHW33_001472"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV1644995.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDDGLKRCLQKLRNAH"
     gene            24554..27739
                     /gene="rne"
                     /locus_tag="RHW33_001473"
     CDS             24554..27739
                     /gene="rne"
                     /locus_tag="RHW33_001473"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV1644996.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAVPA
                     QPGLLSRFFGALKALFSGGEETKPSEQAAPKAETKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQTQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVTAVKPEITIEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27934..28887)
                     /gene="flgL"
                     /locus_tag="RHW33_001474"
     CDS             complement(27934..28887)
                     /gene="flgL"
                     /locus_tag="RHW33_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV1644997.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNTQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGEYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28899..30542)
                     /gene="flgK"
                     /locus_tag="RHW33_001475"
     CDS             complement(28899..30542)
                     /gene="flgK"
                     /locus_tag="RHW33_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV1644998.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTIDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMVNGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDEGEDFFAIGKPA
                     VLQNTKNNGNVAIGATITDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30608..31549)
                     /gene="flgJ"
                     /locus_tag="RHW33_001476"
     CDS             complement(30608..31549)
                     /gene="flgJ"
                     /locus_tag="RHW33_001476"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV1644999.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31549..32646)
                     /gene="flgI"
                     /locus_tag="RHW33_001477"
     CDS             complement(31549..32646)
                     /gene="flgI"
                     /locus_tag="RHW33_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV1645000.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32658..33356)
                     /gene="flgH"
                     /locus_tag="RHW33_001478"
     CDS             complement(32658..33356)
                     /gene="flgH"
                     /locus_tag="RHW33_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV1645001.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33409..34191)
                     /gene="flgG"
                     /locus_tag="RHW33_001479"
     CDS             complement(33409..34191)
                     /gene="flgG"
                     /locus_tag="RHW33_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV1645002.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVLLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34363..35118)
                     /gene="flgF"
                     /locus_tag="RHW33_001480"
     CDS             complement(34363..35118)
                     /gene="flgF"
                     /locus_tag="RHW33_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV1645003.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALLQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35138..36343)
                     /gene="flgE"
                     /locus_tag="RHW33_001481"
     CDS             complement(35138..36343)
                     /gene="flgE"
                     /locus_tag="RHW33_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV1645004.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWEVYTQDSSDP
                     TGTADRAMTLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36368..37063)
                     /gene="flgD"
                     /locus_tag="RHW33_001482"
     CDS             complement(36368..37063)
                     /gene="flgD"
                     /locus_tag="RHW33_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV1645005.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37075..37479)
                     /gene="flgC"
                     /locus_tag="RHW33_001483"
     CDS             complement(37075..37479)
                     /gene="flgC"
                     /locus_tag="RHW33_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV1645006.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37483..37899)
                     /gene="flgB"
                     /locus_tag="RHW33_001484"
     CDS             complement(37483..37899)
                     /gene="flgB"
                     /locus_tag="RHW33_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV1645007.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38054..38713
                     /gene="flgA"
                     /locus_tag="RHW33_001485"
     CDS             38054..38713
                     /gene="flgA"
                     /locus_tag="RHW33_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV1645008.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSSQLAGISDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLGAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38789..39082
                     /gene="flgM"
                     /locus_tag="RHW33_001486"
     CDS             38789..39082
                     /gene="flgM"
                     /locus_tag="RHW33_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV1645009.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNPRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTSKIADALINEAQQDLQSN"
     gene            39087..39503
                     /gene="flgN"
                     /locus_tag="RHW33_001487"
     CDS             39087..39503
                     /gene="flgN"
                     /locus_tag="RHW33_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV1645010.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(39543..41078)
                     /gene="murJ"
                     /locus_tag="RHW33_001488"
     CDS             complement(39543..41078)
                     /gene="murJ"
                     /locus_tag="RHW33_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV1645011.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41188..42111)
                     /locus_tag="RHW33_001489"
     CDS             complement(41188..42111)
                     /locus_tag="RHW33_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV1645012.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITEMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42113..42760)
                     /locus_tag="RHW33_001490"
     CDS             complement(42113..42760)
                     /locus_tag="RHW33_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV1645013.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42771..43355)
                     /gene="rimJ"
                     /locus_tag="RHW33_001491"
     CDS             complement(42771..43355)
                     /gene="rimJ"
                     /locus_tag="RHW33_001491"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV1645014.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43591..44799
                     /gene="mdtH"
                     /locus_tag="RHW33_001492"
     CDS             43591..44799
                     /gene="mdtH"
                     /locus_tag="RHW33_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV1645015.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44863..45510
                     /gene="grxB"
                     /locus_tag="RHW33_001493"
     CDS             44863..45510
                     /gene="grxB"
                     /locus_tag="RHW33_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV1645016.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45644..46204
                     /locus_tag="RHW33_001494"
     CDS             45644..46204
                     /locus_tag="RHW33_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1645017.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46310..47356
                     /gene="pyrC"
                     /locus_tag="RHW33_001495"
     CDS             46310..47356
                     /gene="pyrC"
                     /locus_tag="RHW33_001495"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV1645018.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEHQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47430..47675
                     /gene="dinI"
                     /locus_tag="RHW33_001496"
     CDS             47430..47675
                     /gene="dinI"
                     /locus_tag="RHW33_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV1645019.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        11523 a        12920 c        11872 g        11428 t
ORIGIN      
        1 ctggactggt gaacggaggt gattattttt ataataacct ttcattcaca gtcaccaggt
       61 acaacggcat catggcaact gactcaacac aatgtgtaaa aaaaagccgt ggccgcccaa
      121 aagtgttcga cagggatgcc gcgcttgata aggccatgaa attgttctgg caacacggtt
      181 atgaagcgac ttctcttgcg gacctcgtcg aagcgaccgg agccaaagcg cccacgctat
      241 acgcggaatt taccaacaaa gaggggttat ttcgtgccgt tctcgaccgc tatatcgatc
      301 gttttgccgc taagcatgaa gcacagctgt tttgtgaaga gaaaagcgtg gagtctgcgc
      361 tggctgacta ttttgctgcc atcgccaact gctttaccgg caaagacacc ccggcaggct
      421 gcttcatgat caacaactgc accaccctct ccccagattc aggagatatc gccaatacgt
      481 tgaaatcacg ccatgcgatg caagagcgca ctttgcagca gtttttatgt caacgacaag
      541 cgcgcgggga aatcccgacc cactgtgacg tgacacatct ggcagaattc cttaattgta
      601 ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc gctggaaaaa ctgatgcaga
      661 ttgccggaac gactttgcgt ttatggcccg aactggtgaa ataagtcgca aaaaaagccc
      721 ctcagcctga gcgaatgagg ggccaaattg cagagtacag ccttgttata ctttgttatt
      781 caaaatcagt tgcccgttac tatctagcgg gatctgcgta cccggatcgc ggtccatgcg
      841 gattttgccc tgctgatcgc caatcttata ggtcacatca taaccgagca ttttttctga
      901 cttgtcatac accgttttac aacgctgttg cgtagtcgtg taagtatcgc tttcctggag
      961 agagccctgg atctggttac cggcatatcc cccccccagc gcccccacaa cagtggcgac
     1021 atctttacca cgaccaccac caaactgatg ccctatcacg ccgccagcaa cagcgccgag
     1081 caccgacccg gtaatgcgat tttcatcctg caccggtcga cgatgggtca ctgtgacgtt
     1141 gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt ggggttgcag aaacaacctg
     1201 agcgtattgc gggccacgtt caaacacgtt cagactggcc actgccgcta cgccaagcgc
     1261 agctgcgaca ccaatcccga tacccgccaa cattgattta ttcacgggaa tacctccttc
     1321 tttgcagtta tgccaatcat gcaagcgaag gagaaaagca gctatcagac agaagatgaa
     1381 aagcgcggga atggctggaa atacttaagg attcggagga gtctgaagga atgcctgatg
     1441 cgacgcttaa cgcgtcttat caggcctacc gctcgtacca tttgtccagg gcggatgagg
     1501 cgtttatgcc acatccgcca gtgtacgtcg attaatgcaa cttcaaacgc ggacggataa
     1561 cgcggttaat actccccacc agcatcatta atccggtttt aaagtaacca tgcagcgcaa
     1621 tctgatgcat tcggtatagc gagatatata caaagcgcgc aattcgtcct tcaatcatca
     1681 ttgagccgcg cgtcaggtta cccatcaggc taccaacggt ggagaagttc gacagcgata
     1741 ccagtgaacc atgatcttta tactgataat ttttcagcgg cttaccgttc atctgcgcca
     1801 gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc ctgagcacgc ggcggaacaa
     1861 agcccccttc cggacgcggg catgacgcgc agtcgccaat agcgtaaata tctggatcgc
     1921 gggtggtttg cagcgtcggt tccaccacca gctggttgat acggttagtt tcaagaccac
     1981 cgatatcttt caggaagtct ggcgctttga tcccggctgc ccataccatc agatcagcct
     2041 caatatattc accatcttta gtgtgcaggc cgccttcatc agcactggtg accatggttt
     2101 gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg ggccgcagca gagatacgtg
     2161 gcggtaatgc aggcaaaata cgttctcccg cttctaccag cgttacgttc agggcttcgt
     2221 tggtcaggcc tttgtaaccg tagctgtgca gttgcttgac cgcgttgtgc aattcagcgg
     2281 agagttctac acccgtcgcg ccgccgccga caatcgcaat gttcactttg ccattcgcgc
     2341 ccaggttggc ggagtatttc aggaacagat tcagcatctc ctggtggaag cgacgcgcct
     2401 ggtgcgggtt atcgaggaaa atgcagttct ctttgacacc tggcgtattg aaatcgttag
     2461 aggtgctacc cagcgccatt accagggtgt cataggcgat tttacgttcc ggaaccagca
     2521 gttcaccttt ctcatcgcgc agttctgcaa tagtgattgt tttcgcttca cgatcaatat
     2581 caatgacgga acccagctgg aactggaaac catgattgcg cgcatgggcc agatagctca
     2641 acgcatcgac gccttcatca agcgagccag tcgccacttc gtgcagcagc ggtttccaca
     2701 ggtggctgtg attacgatcg accagcgtaa ttttggcttt tttcttgcgt cccagcttat
     2761 gccccagctg tgttgccatt tccagcccac cagcaccgcc gccgacaatc acaatttttt
     2821 tcaatggcgt agtcaacgtg acccccttaa atttattaac caattgttaa ttaatggtta
     2881 ttagcatagc tcttaattaa caacaggtta cagcaatgaa aatgttcttc gctattgaga
     2941 ataacatgaa tggtgcattg gtcataccaa aattgatgtt aatcaagttt tgttgctgaa
     3001 aagataggcg actttaccgg gtgagatcaa aaaacagagt ttattgttta ttatttgagc
     3061 taatttgtga gagtgatata gagtgagggg tgtatgtagg gcgttcggta ggcctgataa
     3121 gaggcgcatc aggcattgat gctcgactgt cggatgcggc gtaaacgcct tatccgactt
     3181 acagaatccg gagcgcatca ggtaccggca aggttacctg atgcgacgag ggcatttacc
     3241 cgagggtttt gaacgcttta atgcgctgta aatgcgggga gatattcttg aatttgtgcg
     3301 tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc
     3361 tgttaagcgc ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga ttcttctcac
     3421 ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc
     3481 cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg atgtcgcaaa
     3541 gaaaaccaat ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt agcggacgct
     3601 cgtcaacgtt cagttgcaac attttgtcca cttctttaag cagatgctgc atatcatgtt
     3661 tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta
     3721 agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg
     3781 cttcctcaca taagccaggg ttagtgacct gctttctctt cctgccagcg ttcatgtaac
     3841 tgattcagac gggcgctgat cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa
     3901 aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga cagattatct
     3961 aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca acccgcatcc
     4021 agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa
     4081 tcgtcagaga aaatcacgcc gtcaaaaccc agttcctgac gcaaaacggt tttcagccag
     4141 taggaagaac cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata
     4201 atggcatcga gtttattctc gcggattaac gtgctgaaaa ccgacatatc tttggcacga
     4261 atttccgctt gcgggcgtgg gtcgcacggc gtctctttgt gtgaatctgc agttactgca
     4321 ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat cccatcaata
     4381 aaccgactgg cgattgccag ggctttttct gggtcggcat gataagaacg ctcgccaatc
     4441 gccgcgctga tatgtcctac atccagcact ggcgcaaagc tgatatcaat atccatagcg
     4501 atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt
     4561 cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc gcgaaaacgc
     4621 tggacgcgtc caccttcttg atctaccgcc accaccagat gattgcgcga tgctgcgcgg
     4681 atctggcgca ccagttcacg caactgggca ggatcatgat agtttcgcgt aaagagaatc
     4741 agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag ttcgtaacct
     4801 tcgacatcca acattactgg acccacactg ctctccttat tgttttatta atagctgccg
     4861 ccaggtgtca tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc agcggtactc
     4921 aaaccaccct gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa
     4981 ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc gatgctgttc
     5041 aatattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc
     5101 ccagtcgatg agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat
     5161 atgcaatgga cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat
     5221 tcgcagccaa cccactgtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa
     5281 cggcaacaac gttattcgcc agccaaaacg tggttgttga tgtagataat acagcaagcc
     5341 tgccagttcg ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac
     5401 catccagtca tggagatata aatgcggctt cggtgcaatg catgcgggta gttgtgataa
     5461 agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac
     5521 aacaaaacgc tggccctgat gttcaatgag aaaactcccg ccactaagcc cactattaaa
     5581 cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg
     5641 attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca
     5701 tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgctggaa tacagcacgt
     5761 aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtgcct aaactatcct
     5821 gcggcgacaa acctaactgt tgcttagcca tcgacagctg ctgggcggaa accagggtaa
     5881 atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg
     5941 aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt
     6001 cagccccaag catcttactg accatcggct gcattgcgcc attccagtca taatggcgaa
     6061 tatgcggcgc aggcggtgca gtttgatctt cgtgctcaat tgggcctggc tgctgcggga
     6121 tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg
     6181 ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca
     6241 tcgccagcgc agtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac
     6301 aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctacc gtacagcgtt
     6361 accgccgaat gtgcgggaat ggtaacgctg cgtggccttt ccagaggatg catctccagc
     6421 cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta
     6481 taaagcgttg aggatgctga aggttgaata tcagacgtcg aaaggaccgg cttttctgca
     6541 ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga
     6601 atttccggat gtgaacgaca gcccaccagc agtaacaacg ccagagacac caacccaaag
     6661 catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac
     6721 ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg gtgttcatga
     6781 tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga
     6841 tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc ggaatgagga
     6901 tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta
     6961 ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata
     7021 tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg
     7081 ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact
     7141 cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc
     7201 tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg
     7261 tatatttctc atttgcattt acaaacaaaa ttattcgcac actaaatata accgctcatc
     7321 accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac
     7381 acaatttaac agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg
     7441 catagcattg gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt
     7501 gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc
     7561 tacctctgcg ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt cggtcactat
     7621 tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa
     7681 cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgcgg
     7741 attccagatc gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa
     7801 tctgggcgac gcactttctg atatggcact gtttgaacgc gtagaagtag tgcgtggcgc
     7861 gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca
     7921 cgcgaccagt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga
     7981 acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat
     8041 tgtcggcggc taccagaata acgactcatg gctggaccgc tacaatagtg aaaagacctt
     8101 cttctccggc attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga
     8161 atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct ggaatactga
     8221 tggcagcagc aacagttacg atcgcgcacg cagtaccgca cctgactggg cgtacaacga
     8281 taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc
     8341 gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc
     8401 ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt
     8461 tgattatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt
     8521 gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag
     8581 ttacagcaaa caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat
     8641 tggcagtttc tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct
     8701 ggcgcaggac gataccacac atatgaaatc gttatatgct gccactcgtg tcacccttgc
     8761 cgatccgctg catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac
     8821 ttacagcatg gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga
     8881 caactggtcg acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag
     8941 ttcaggcaaa tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga
     9001 ctggatgaat agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt
     9061 cgctcagtcc accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt
     9121 ggatgggaca gtcagtaaag gtgtggaatt tgaactcaac ggcgcaatta ccgacaactg
     9181 gcagctgaca tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa
     9241 tcctaatctg cctcgcacca cggttaaaat gttcaccagc taccggttgc ctgtcatgcc
     9301 agagttgacg gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac
     9361 accgtatggc accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg
     9421 ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac
     9481 ctacgatacc aacgtggaag gttctatcgt ctacggcgca ccgcgtaatt tcagcattac
     9541 cggcacgtat caattctgaa taacacctgt aaaaaaggca gccatctggc tgccttagtc
     9601 tccccaacgt cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt
     9661 atcggattta gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc
     9721 gcccagtttc ttcaggccgg cctgatccac tttagacaca tcagcaacgc tgacgcgcag
     9781 acgggtaata catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag
     9841 agccggtgcc atttcgctgg tacctgtcgc ttttgcatct tcagtcgcgt cttcacgacc
     9901 cggcgttttc agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc
     9961 ataaccgata ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacaat
    10021 gaagtcgata agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat
    10081 tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat
    10141 gaaggagaac tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat
    10201 cataataccg cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc
    10261 ggcagctggc agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg
    10321 gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta
    10381 ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag
    10441 gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc gggttctggt aagcagccca
    10501 ctgagagaag gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc
    10561 agtaaagatg gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc
    10621 aagatactca ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc
    10681 ggagataatc cctccgagta cgcctgtatc cgccaggtgt ttagaggcga tttcttcagc
    10741 aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc
    10801 aacaactgcg gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat
    10861 cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga
    10921 tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat
    10981 aggcagtacg gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc
    11041 attcttaaac ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg
    11101 tggcaagggg ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac
    11161 agagtaaaat aattcagtgc caatttgttt gacggctatc acgtttcaac ttcagatcat
    11221 tggagaaatt gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac
    11281 cgcccaattt tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa
    11341 actcattcaa cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt
    11401 atcggtggtt acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc
    11461 tgcaacgtca cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag
    11521 gtaaggtgag tcagtttcca ccagtaaccg atccaggggg acataacgcg cagcatcgcg
    11581 cagttgctcc gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc
    11641 cagtaatttg cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc caccgcaatc
    11701 cgtcactttt tcttcgcgca gaattgccag cgtatcggcg cgggcgtcac gggtatggac
    11761 gataaccggc ttgttcagtt cacgaccaat ttggatatga tggatgaagg actcttgctg
    11821 acgtacttta gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac
    11881 accctcttct gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag
    11941 tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatgtg
    12001 taaataaccc ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc
    12061 tttcgccaga acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg
    12121 gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa
    12181 cgcccggttg caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt
    12241 tgatgcctgt gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct
    12301 gcaggcgcga gggagaaaga tggtttgcca gttcgacgac caggcccggc acatcaacat
    12361 tggtcacctg cgcagcgcca tgatggcgtt ttagcgcatc catcagcaat gttgccagcc
    12421 agtgcaaacg cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc
    12481 ccgatggcac gctatatgcc aacgcctgac acaatgtttc acgagcctgc cagttatctc
    12541 cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat gcggcaagta
    12601 atgcatcctg tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg
    12661 gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct
    12721 cgcgggtagc caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg
    12781 cgttagccgc ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac
    12841 ctaagcgtgc gtgctcattc agcttttcgg tgacctcacg taccgcatca acgcccagcg
    12901 tatttttccc tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca
    12961 actgacatcc acgacagtga ccgcaacttt tgtggccctg cggttgttgg cagagtaaat
    13021 aacggctcag ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagta
    13081 gcgcatggtg acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta
    13141 accatggata ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat
    13201 tgcatccatc acggcctcca gcggctgggt ggcatcaatg gtatgaatac ttttatcttg
    13261 tgcagccagt tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc
    13321 aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc
    13381 gagatagagc gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag
    13441 catatgttgg tcaataccac gtccgccgcc ctgatacgcc tgagtggaga gatcgtggcg
    13501 atcgccaatc acccaggtgc cgttagccag cgcaggtttg atgaccgttt ctaccagttg
    13561 aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac
    13621 cgatttgata tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc
    13681 ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc gtctcaacca ccacattacg
    13741 cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt
    13801 tttccttaag cactttcaga taatcctgca cagacttgtt atgactggca agattggtat
    13861 taaacgtgtg accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat
    13921 gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat tgcacctggc ggcaaaccgg
    13981 taatggtata ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat
    14041 tataacgctc tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc
    14101 gtaaacggtt gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt
    14161 tttcgataat tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg
    14221 cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta
    14281 acgcgacatc ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa
    14341 tccactcagg gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca
    14401 gcgtgtgctt gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc
    14461 cttctaccag tcgcagcggg aactgtgctt ctttaccgct ttccaacaat ttcagcatct
    14521 cgcgcacggt catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg
    14581 gttcgatacg cagcaaccat tgaaaaaccc gtgggcgatt gatgatctta tcggcataaa
    14641 gctgttcacc aagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt
    14701 tgataagcaa tttgctgtcg gctagatagc gaaccttcca gacgcccaca ccagcggcga
    14761 tacccagtac caccagcaat aacaagatta tcaataacac ttttttcatg actaattcgg
    14821 gcgctcacaa agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc
    14881 acaggcacgt acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg
    14941 caacgcctct tccagagaag cttgcacttc gacaagctga taagaggact gtgccagcaa
    15001 acggatacag aattgtcgca taatgccgtt aacacctgcc tgatccagtc gcggcgtata
    15061 aactacgttg cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc
    15121 gctgtcaagg accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac
    15181 ttgctcaagc cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac
    15241 cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataagc
    15301 cgtaacggag agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc
    15361 actaccgcga ctgatcacga ctttcagtac accattttgc tgttctgctg ccagcgtttt
    15421 catctcttgt tcaagctgag gccagaagtc acaggaaatc atcaaccgct gacaagcatc
    15481 ctgtagtcgc tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt
    15541 ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt
    15601 atgaccgtta attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg
    15661 ttcactacgg aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta
    15721 taagggtgga aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg
    15781 aagccgaagg agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt
    15841 acgaaatcca gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc
    15901 tgatcgcgca gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg
    15961 tgaccggtca tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg
    16021 gttttcaccg cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgagcgtta
    16081 acgtagccaa tctgactcgc ttcaatgcct gcatcacgta gagcatttgc cattgccaga
    16141 gctgcgcctg cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca
    16201 aagccgacga gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca
    16261 agtaccagca taccggcacc atcgcccagt acgaagccat cacgctcttt atcccacggg
    16321 cggctcgccg cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca
    16381 ccaacaccca gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg
    16441 ccatacgcga taatacgcgc agcatggcca atgttgtgca cgccggaagt acaggcggtc
    16501 gcgatagaga tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg
    16561 ttcacaatcg ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga
    16621 gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca
    16681 atgcgggttg cgttctcttc cgttatttca aggccagaat cctgcatggc ctgaacgcca
    16741 gcgacaattc catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg
    16801 tcctcacagt taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg
    16861 aaatggtcga ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct
    16921 acggtattgc cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac
    16981 acgtttgtcc tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg
    17041 cggtcgaacg accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg
    17101 gcagcctgaa cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct
    17161 tcttccagag ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg
    17221 aaagaagcat tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc
    17281 ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg
    17341 atggttttcg tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac
    17401 cacgatttta cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat
    17461 tattactcat aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag
    17521 accatgtaca tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg
    17581 gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga
    17641 acctgcgcca ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca
    17701 ataaagcccg gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt
    17761 gatttactga agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt
    17821 cccatggtac caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata
    17881 gcgcgcatta ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg
    17941 ttccactctt catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg
    18001 accaggatat ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat
    18061 gccgggtcgg tcacattcaa catcagacct ttgccgttag cacctaaata atcactgatc
    18121 gcctgagcgc cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc
    18181 gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct
    18241 tcaaaattca tgattttcct cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg
    18301 ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt aagcactttg
    18361 cccgggccga cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc
    18421 gtccactgaa ccgggttata caactgacgt accagtgcgt cacgaatggc atcaccattg
    18481 gtttcgcatt tcacatcaac gttattcaca acaggaactg ttggtgcgtt aaaggtgatt
    18541 ttcgctaatt ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt
    18601 acgctcactg gtaacggcag agcgcgtttt gcgcccgcag ctttacaggc agcgccagca
    18661 cgctcaaccg cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc
    18721 ggagaaacga cctgaccttc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc
    18781 agaccgatga ttgccgccat agcgcccgtg ccttccggta cggcttcttg cataaacttg
    18841 ccgcgcatct caaccagacg caccgcatca gcgaaatcaa tcacaccagc gcaaaccagc
    18901 gcggagtatt cccccaggct gtgaccggcc atcaatgccg gtgctttacc gccctgctgc
    18961 tgccatacgc ggtacagcgc aacagatgca gtcaacagcg ctggctgggt ttgccaggtt
    19021 ttattcagtt cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc
    19081 gcagaagctt cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc
    19141 attccaacgg tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa
    19201 tccttatcct agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca
    19261 agcagaacca actgccccgg cttaatacgc ccgtcgcgta cggcttcatc cagcgcgcac
    19321 ggtacagaag cggcagaggt attaccgtgg cgatccagcg tcacgacgac attgtccatt
    19381 gacatgccga gttttttcgc cgttgcgctg ataatacgca ggtttgcctg atgcggaacc
    19441 agccagtcca gttgggaacg gtcaagatta tttgcttcca gcgtctcatc aacgatgtgc
    19501 gccagttccg ttaccgcgac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa
    19561 ttctctggat tcacgcggtc ggcgtttggc agcgtcagca attcaccata actaccgtcg
    19621 gcatgcagat gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc
    19681 gcgccatcgc caaaaataat aatagtccca cgatctgttg gatcgcaggt gcgcgccagt
    19741 acatcggaac cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct
    19801 acgcttaatg cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct
    19861 ttaatgccca acatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa
    19921 gtcgttgcca caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg
    19981 cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt
    20041 tcgcggatac cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa
    20101 tcggcgtttg tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac
    20161 atgtacgctc agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc
    20221 agcgtcctgc taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg
    20281 gtatacagat tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac
    20341 tgcctgttca atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac
    20401 cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa
    20461 tcgcctcgtc aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt
    20521 tttcccttca ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt
    20581 ctttaatgtg acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa
    20641 caactcattg gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc
    20701 tgaggcatcc cgaatactat cgagaccctt tacttcttct tcaccaatat tgagcaacgc
    20761 cacgcgagga ttgggaattt ccaccacctc ttcagccagg actgagccca taatggcaaa
    20821 ttgcaccaac attgtgctgt cacaatcgac gttggcccct aagtcaagga ccaccgtttt
    20881 gcccttttgc tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg
    20941 tttgagtaat aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc
    21001 ttgcgctcga ccttctttca ccagctccag ggccacgcgc attgaactcc cacgactggc
    21061 gcggatagct tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg
    21121 cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt
    21181 gccgactaaa agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac
    21241 tgtcacggaa gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa
    21301 ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc
    21361 ttcatcacgc agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg
    21421 gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg
    21481 actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc
    21541 tgttgtacgg ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc
    21601 accaattact tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcgcttct
    21661 tcaggcagtt caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc
    21721 accggaacta ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt
    21781 tcaccgaatt cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg
    21841 tctgaacgca caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg
    21901 caacgctgac actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt
    21961 tggttatcga tagcgaacga catggagcat tccacatcac tgtccacact gactacggat
    22021 tcggcgacgc gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga
    22081 gccgtacgaa ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat
    22141 taactttgta acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata
    22201 attcgcacac attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac
    22261 ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa
    22321 aaaatatgcc taaacttatt ttagcctcca cctcgccctg gcgtcgcgct cttctggaaa
    22381 aactgcaaat ctcttttgaa tgtgcagcac ccgaggtcga cgaaactcca cacagcgacg
    22441 aatcaccgcg acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac
    22501 gttatccgga tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca
    22561 ccggtaaacc gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata
    22621 tcgtcacctt ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag
    22681 aagtggagcc ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg
    22741 tgcgtaaaga gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta
    22801 ctttgtttga gcgtttagag gggcgtgacc ctaacacgct ggttggttta ccgctaatcg
    22861 cgctgtgtca gatgttacgt cgggaaggga aaaacccgct gatgggatag ttttttacgc
    22921 cggatgcggt tacttcgcat ccggcaatcg gtgctggcgg cctgatgcgg cgtaaacgcc
    22981 ttatcaggcc aaccgcccat tattaggctt atatgtttgt atcagtgcgc gttacgcagc
    23041 ttttgcaaac aacgcttcaa accatcatcc atcggcgctt cgatacgcat cacctcaccg
    23101 gtccccggat gagtaaactt caacgctgca gcgtgcagga acaggcgatt taatcccgtg
    23161 cctgcttcag tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc
    23221 ggatgacccg catattgtgt atgcacacgg atctgatgag tacgccctgt taccgggcta
    23281 caacgcacca gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt
    23341 ttgccttcct gactcacacg cacgatacgt tcgccgcttt gcagaatatt tttcagtaac
    23401 ggtgcttgaa cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct
    23461 ttttgcatcc ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc
    23521 gctaccagca acacacctga ggtgtcccgg tcaagacgat gaaccagttc aaggaaccgc
    23581 gcttccgggc gcaacgcccg caaaccttca ataacgccga agcttaaacc actgccgcca
    23641 tgtaccgccg taccggaagg tttattcagc accaggatgt gatcatcttc atataagatg
    23701 acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca
    23761 gcaacgcgaa ccggtggaat acgcacctca tcacccgctt cgagtttata ttcaggctta
    23821 atacgttttt tgttcacccg cacttcgcct ttacgcaaaa tacggtaaat catacttttt
    23881 ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg
    23941 gtgatagcaa caatttttac ggatggagtc tctgttttca tggttggcga ttctaattag
    24001 ccaacaggat tcgcgccact catttttcta tgcttatatt tacttttgca ccttattact
    24061 tcactgcgtg atcactttat tgatggttat taaaccaatc accagcaaga agtgaaaaaa
    24121 ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc tataacaagg cttgcagtgg
    24181 aataatgagg ccgtttccgt gtccatcctt gttaaaacaa gaaattttac ggaataaccc
    24241 attttgcccg accgatcatc cacgcagcaa tggcgtaaga cgtattgatc tttcaggcag
    24301 ttagcgggct gcgggttgca gtccttaccg gtagatggaa atatttctgg agagtaatac
    24361 ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc aaccgacact
    24421 ctgcgcctct ttgagctgac gataaccgtg aggttggcga cgcgactaga cacgaggcca
    24481 tcggttcaca cccggaaagg cgttactttg cccgcagctt agtcgtcaat gtaagaataa
    24541 tgagtaagtt acgatgaaaa gaatgttaat caacgcaact cagcaggaag agttgcgcgt
    24601 tgcccttgta gatgggcagc gtctgtatga cctggatatc gaaagtccag ggcacgagca
    24661 gaaaaaggca aacatctaca aaggtaaaat cacccgcatt gaaccgagtc tggaagctgc
    24721 ttttgttgat tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa ttgcccgcga
    24781 atatttccct gctaactaca gtgctcatgg tcgtcccaac attaaagatg tgttgcgtga
    24841 aggtcaggaa gtcattgttc agatcgataa agaagagcgc ggcaacaaag gcgcggcatt
    24901 aaccaccttt atcagtctgg cgggtagcta tctggttctg atgccgaaca acccgcgcgc
    24961 gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc gaattaaaag aagcactggc
    25021 aagccttgaa ctgccggaag gcatggggct tatcgtgcgc accgctggcg tcggcaaatc
    25081 tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa cactgggaag ccatcaaaaa
    25141 agccgctgaa agccgcccgg ccccgttcct gattcatcag gagagcaacg taatcgttcg
    25201 cgcattccgc gattacttac gtcaggacat cggcgaaatc cttatcgata acccgaaagt
    25261 gctcgaactg gcacgtcagc atatcgctgc attaggccgc ccggatttca gcagcaaaat
    25321 caaactgtac accggcgaga tcccactgtt cagccactac cagatcgagt cgcagatcga
    25381 gtccgccttc cagcgtgaag tccgtctgcc atccggtggt tcgattgtta tcgacagcac
    25441 cgaagcgtta acggccatcg acatcaactc cgcacgcgcg acccgcggcg gcgatatcga
    25501 agaaaccgcg tttaacacta acctcgaagc tgccgatgag attgctcgtc agctgcgcct
    25561 gcgtgacctc ggcggcctga ttgttatcga cttcatcgac atgacgcctg tacgccacca
    25621 gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc gtattcaaat
    25681 cagccatatt tcgcgctttg gcctgctgga aatgtcccgt cagcgcctga gcccatcact
    25741 gggtgaatcc agtcatcacg tctgcccgcg ctgctccggt accggtaccg tgcgtgacaa
    25801 cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga aagagaacac
    25861 ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct tacctgctga atgaaaaacg
    25921 ttctgcggta aatgccattg aaactcgtca ggacggtgtg cgctgtgtaa ttgtgccaaa
    25981 cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg cgtaaagggg aagaaacccc
    26041 aaccttaagc tacatgctgc cgaagctgca tgaagaagcg atggcgctgc cgtctgaaga
    26101 agagttcgct gaacgtaagc gtccggaaca acctgcgctg gcaacctttg ccatgccgga
    26161 tgtgccgcct gcgccaacgc cagctgaacc tgccgcacct gttgtagccc cagcaccaaa
    26221 agctgcaccg gcaacaccag cagttcctgc gcaacctggg ctgttgagcc gcttcttcgg
    26281 cgcactgaaa gcgctgttca gcggtggtga agaaaccaaa ccgtccgagc aagcagcacc
    26341 gaaagcagaa acgaaaccgg aacgtcaaca ggatcgtcgc aagcctcgtc agaacaaccg
    26401 ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt actgaaggca gcgataatcg
    26461 cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag actgccgaga cgcgtgagag
    26521 ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc aagcgccgcg
    26581 tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag acgcaacaag aagcgaaggc
    26641 gctgaatgtt gaagagcaat ctgttcagga aaccgaacag gaagaacgtg tacgtccggt
    26701 tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc aaagcgtagc
    26761 cgaagaagcg gtagtcgcac cggtggttga agaaactgcc gctgccgaac caattgttca
    26821 ggaagcgcca gctccacgca cagaactggt gaaagtcccg ctgccagtcg tagcgcaaac
    26881 tgcaccagaa cagcaagaag agaacaatgc cgataaccgc gacaacggtg gcatgccgcg
    26941 tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc gtcgctatcg
    27001 tgacgagcgt tatccaaccc aatcgccaat gccgttgacc gtagcgtgcg cgtctccgga
    27061 actggcctct ggcaaagtct ggatccgcta tccgattgta cgtccacaag atgtacaggt
    27121 tgaagagcag cgcgaacagg aagaagtgca agtgcagccg atggtgactg aggtccctgt
    27181 tgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt gaagaagtgg ctgaagtcgt
    27241 agaagccccc gttcagattg ccgaaccgca accggaagtg gttgaaacga cgcatcctga
    27301 agtaattgcc gccgcggtaa ctgaacagcc gcaggtgatt accgagtctg atgttgccgt
    27361 agcccaggaa gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag agacggcaga
    27421 tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag ttgttgctca
    27481 acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtaa ccgcggttaa
    27541 accagagatc accattgagc ataaccacgc taccgcgcca atgacgcgcg ccccggcacc
    27601 ggaatatgtt ccggaggcac cgcgtcacag tgactggcag cgccctactt ttgccttcga
    27661 aggtaaaggt gccgcaggtg gtcatacggc aacacatcat gcctctgccg ctcctgcgcg
    27721 tccgcaacct gttgagtaat aattagctca agtaatcaag ccctggtaac tgccagggct
    27781 tttttatttc atctttgaat caacatatta aaattcatcg aatggcatcc ttgctaacca
    27841 acaatgcaaa ataggcaaaa aaatgcccgc cccggcgatc ccagagcggg cagacaaaac
    27901 atacccagtt tcatgatatg ttttaaagcg agcttatttg ttgagctgga agagcgacaa
    27961 tccctgcata tcggtaaatg ctttatacga tgcctgcaat gccgtttgct gcatgatgta
    28021 agatgaaata gttgcattcc agtccacatc aaccagatcg ctcatctgct gcgtttgccc
    28081 taaagcgcga tcgctaccta atgaatccag cgactccagt tcgttcagct gcgtgcctaa
    28141 ttccgcgcga acggtcagca cattgttcag cgagtttttc agtccgcggt tggttttatc
    28201 taacgccgca gcggcggttt ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc
    28261 tgcgatggca ctatccagca tggcaaaaag attggtttca gaagcgctac cgtctggttc
    28321 cgctaccgcg ttgctggtaa tactgtcgaa aattttgtca cccgtgtgcc ctatcaccat
    28381 cgaacgcgaa gcatcgacct gttgtttaat actttctgct ccgccaacat attcgccgtc
    28441 tgcctcacta aacggcgcag tttctgtttt ataaccggca aaaatgtagc gcccgttacc
    28501 gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat ccgtagccag
    28561 cgaggcccgg tcatcgtcac tcaaagtgcc attgctggcg tagacaattt tttcctgagt
    28621 attctggata gctgtggtga cctggctaag tacactctct tccagtgaca ctttttgagt
    28681 ggcgaaagta cgcgccagcg tgtactggct gttttgcgcc tgtgcctggg agagaactac
    28741 ggcttgtgat gctgcaatgg gatcgtcaga agggttaacg actcgcttac ctgtcgacat
    28801 ctgctcgccg tacttcatcc attccgcctg agaattggtg atgccacgca tgttttgctg
    28861 gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt aatcagcgca
    28921 tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa
    28981 cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg
    29041 gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt cgcggtttta
    29101 ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc accgcccacc
    29161 gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttgtc gctatccccc
    29221 gcatcttctt cgctcgccat cgctattttc gcttcgtcgg tgattaatac atccatgttg
    29281 acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg cgttcctgta
    29341 aacgtcaact ccagaccatc aaatgccact ttaccgttgg catctggcgt caccgtaaaa
    29401 gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga gattttgtaa
    29461 tctgtcgcca gtaccgcgga ggcatcagtt atcgtggcac cgatcgcaac gttaccgttg
    29521 tttttagtgt tttgcagaac cgcgggctta ccgatagcaa agaaatcttc accctcatcg
    29581 ccgttggcat caaatccggc tttgtgttgg gtgttgaaag cctcagcaaa tgccagcgcc
    29641 agttgtccaa gcgtattacg cgtctggtcc agatcctgag aacggaatgt cagaatgccg
    29701 cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc cgtcccatca
    29761 acataagcga cagtcgtacg agaagggtcg gcgctggaag gaactgccgc cagttgccgc
    29821 gccgtacttc cctgaaccag tgagtaacca ttgaccatcg tgatgttata agtgccgcca
    29881 tcctgaacgc tgacttctac accaacaatc tggtttaatt cgctcaccag ttgatcgcgt
    29941 tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg ggagatttga
    30001 tcgttcaggc tggcgatttg tttagcgtaa ttgttgatct gatcaacgct ggcacctatc
    30061 gcgatattga cctgtttgtc ctggtcgcga agatactgat cgatggtttt aaactgattc
    30121 accaaccctt ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc
    30181 accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga agaggtactg
    30241 gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcagtcag gccgctactt
    30301 tgcgtctgcg ccgcacgtaa ctggttggta ataaacgcat catactcacg ctgcacacca
    30361 gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt ggcctgcgcc
    30421 ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt attacttgcc
    30481 gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat caagctggac
    30541 atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc cgccggactt
    30601 gagttattca gaacagatta tcaatgttca tactgtaggt tttgctcacc ttgtcgctta
    30661 tcgatttcat ctgctgaatc atgttggtga gtttacgggc atagtgagga tcggtggcat
    30721 agcccgcgtc ctgtagggcc tgcgccccct gctccgcact cgcggcggtc gtcacggcgg
    30781 cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc agatacgagc
    30841 tgtagacccg aaacttcgct tttactttct tcgcttcgcc attttcatat tcagtcgtgg
    30901 tgatttcagt gactggccct ttccagttgc cagaggcttt gacaccaaac aggttatagc
    30961 tcggctcgcc gttttcgcgg cggatttgcc gttgccccca gccagattcc agcgccgcct
    31021 gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg gcgggcagcg
    31081 agagttgcgc gaggaatgct ttactgtcac ctggcagcga atcatcgtag ttacgtggca
    31141 cggccttttg caccagctgc gaaagcgcct gattttgata acgcaccacg gtttcgagcg
    31201 ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct ggcgtcatct
    31261 gcttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt tgggcaatct
    31321 gctggtcata catactggta tacaggcgag tgtgctcgct gctgaacagg ccatcttttg
    31381 gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc acctgacggg
    31441 ccaccggacg gatatttgcc gccgggtctt cgcccgcttt cgcctttagt tcgttgagtg
    31501 attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc agatgatttc
    31561 cagttttgcc cgcagacatc ccgcactttg cattgattgc aaaatagaca tcagatccat
    31621 cggcgtagcg cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg
    31681 tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac
    31741 agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac gattgactgt
    31801 aactgagaga ttcccctgcg ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac
    31861 caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga cattaacctg
    31921 catattctgg atatcggcaa ggaaacggac ctgggaactg ttgccactcg gtacgcgcac
    31981 ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc ggttgatggt
    32041 gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt
    32101 cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg tgatccgtcc
    32161 accgttcagt tggttcacct gaacactgct accgccagcg gaggctcctg cgccgccaac
    32221 cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa gcggtgtcat
    32281 caacaacgta cctccacgca agcttttggc attacccatg gaagaaacca ccacatcgat
    32341 ggtttgcccc tgacgtccaa acggaggaag tgacgctgtc accattaccg cagcgacgtt
    32401 ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga gcatgttatt
    32461 aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccggtgccat ccagccccac
    32521 caccaggcca tagccaatca gtgagttttg ccttaccccc tgaacactgg tgagatcgcg
    32581 aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag agagaaattt
    32641 aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg caaccagccc
    32701 atattttgcg cttcgttaat gtagccattg cctacgtatt caatacgtgc atccgccacc
    32761 tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac gccagagaag
    32821 cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac atgcaggttg
    32881 ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca
    32941 ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg
    33001 ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt agttttaccg
    33061 tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg caacacgatg
    33121 gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg atagccatag
    33181 ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc
    33241 ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt
    33301 agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt ttgcatggct
    33361 atcatcttca aaaatcaata aaccggtgaa cctgccaccg gttatgcctt agagttgcgt
    33421 cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt cgtaagcgcg
    33481 ttgcacctga atcatattga ccagttcttc cgccacattg acgttagacg tttcaacata
    33541 cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg gtgcaccgga
    33601 ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agcccagtgt cattcataaa
    33661 ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt gggttacgct
    33721 gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg tgatcgctgg
    33781 ctgcacctga aaaccaccag ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc
    33841 gtcacgggta taggctgatg agccatctgg caacaacacc tggaaaaagc cctgcccttt
    33901 aatcgcgaca tctttgctgt tgttggtctg cgacaggttg ccctggctgt gtaagcgttc
    33961 agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc
    34021 ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg
    34081 ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt
    34141 ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca taaaatgtcc
    34201 tgtagtggat tagtgacaaa gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta
    34261 tccggcctac aagagctcgc aaaatcgata aattgcgatt gcccccatag acccgataaa
    34321 cttgcgcatc aggcaactta tacttgtcat gtatcctttc aattaactca tcgacagcag
    34381 ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa
    34441 acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc
    34501 cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtaccggtc cacgtgtagc
    34561 ctggctttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctggactt cgctgcccgt
    34621 ggctttcacc agcttcagac gccccactgg cgcaaccgta tttgccggat cgcccggatt
    34681 cagcgccgag attgtgccat cggcagctat cgtgatttcc gccccttccg gcacagcaat
    34741 tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac
    34801 ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca
    34861 gccatcctgc agcaacgcaa cgtccagcgg gcgcgaggtg taatccattt tgccgggcgt
    34921 catgtctgca cccggtgttg acgccgttac caacgtgcgc gtgggcagtg aaagcccttc
    34981 caccggcact gcgcgcaagg cgtttaactg cgcacgaaaa ccgggcgtag aggcattggc
    35041 cagattactg gcggttaccg cctgttgatt cagcgtctgg ctggctgcac ccatcgcagt
    35101 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    35161 tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    35221 cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc gcaccgttgg
    35281 tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    35341 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    35401 gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccaaca accgtaccgt
    35461 catcattgat ttgataactc accagatcgc ccggtttgta tccgttctgg gtggttgcca
    35521 caatgttgtt agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg
    35581 cggggtctgc gccgttaatt gcgccagtgg taatattctc tgttggattt gacgtcagaa
    35641 cgccattggc attaaacacc agcgtcattg ctcggtcagc tgtgcctgtt ggatcactgc
    35701 tatcctgggt gtagacctcc cagttattat ccccggtctt cacaaagtac acgctcatgt
    35761 catgagcatt accctgacta tcgaaaacag tcaccgaacc ttttttgtta tagctatccg
    35821 cattgctggc atcgaatgcg ttaacagagg gaagcggatc actggaattc aggttgatct
    35881 gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg
    35941 tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct
    36001 gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc
    36061 tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa
    36121 cgtccagacc acgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    36181 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    36241 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    36301 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    36361 tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    36421 ccagcgtatt accgttgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    36481 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacgttgtaa gaaccgttcg
    36541 gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtggact ccggcggtca
    36601 gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa
    36661 ctttgtctgc cgcctgttgc aactcaacac caaacggcgt ggtcgtggtc acagcccctt
    36721 cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccgatca
    36781 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg
    36841 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    36901 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc aaagtcagaa
    36961 aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    37021 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    37081 accgagcgta agggttttca gcatcatgct tttcaccgtg ttgagcactt caacattggc
    37141 ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg
    37201 catttttacg tagccctttg catctgccag cggattaccc ggttcataaa ccagtttgtc
    37261 cggtgcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc
    37321 agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcgg gaccggtcac
    37381 gctatcagca ttcgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa
    37441 cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa
    37501 aacgttcatc atgcctttga tttgcccgct caacgcacta aggctcatct ggtattgcag
    37561 gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga
    37621 aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg
    37681 aatgtgttgc gttgaggtca tcgtcagagc aaccacactg gttgcatccc gtccacgttg
    37741 catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc
    37801 ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc
    37861 ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa
    37921 attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg
    37981 ggtcgctatt tatgccgttg atggtcattg cggacaggta caattcacgt tgtagaaatg
    38041 gctgggggcg aaaatgctgg caataaaacg tagcgtggcg atcatcgcga tactgttcag
    38101 tccgttaagt gcggcgagca atctcacatc gcaattgcaa acctttttta gctcccagct
    38161 cgcagggata agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc
    38221 gtgcgagcag ccattgcttt cgatgagcaa taattcccgc ttgtggggca atgtgaatgt
    38281 gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtgcagg ccacaggaaa
    38341 ttatgtggtt gccgcgatgc ccattgtgcg aggaggaaag ctgggagctg gcaatgtcaa
    38401 actgaaacgc ggacggctgg ataccctgcc gccgcgtacg gtgctggata tcaatcaact
    38461 tgttgatgcc gttagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt
    38521 tcgccaggca tggcgggtaa aagcgggaca acgcgtcaac gtgatcgcca gcggtgatgg
    38581 gtttagcgcc aatgctgaag gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg
    38641 ggtgcgcatg gtgtcgggac aggtagtcag cggcgttgtt gatgcagatg ggaatattct
    38701 tataaacctg taatctgtta aagattaccc gtcccttgcc gataaataag caacacatga
    38761 taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc
    38821 cgtaagcacc gttcaaccgc gcgaaaccac tgacgcgccg gtaacgaacc cccgggcggc
    38881 aaaaacaaca gcatccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat
    38941 gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg
    39001 taacggtgaa ctaaaaatgg acaccagcaa aattgccgat gcgctgatca acgaagcgca
    39061 gcaagacttg cagagtaatt aatcgtatga cgcgtcttgc agagatcctc gaccagatgt
    39121 ccgctgtgct taacgatctc aaaacggtaa tggatcaaga gcagcaacat ctctctatgg
    39181 ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcaa
    39241 cgttggatta cctcgaacag ttacgcagga aagaacccaa tactgcaaat agcgttgata
    39301 ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa atgaatcaac
    39361 ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga
    39421 aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg
    39481 gcagtaaaaa gatttcgatc tgaagattat ctccggcctg cactgcaggc cggaatgcat
    39541 tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg
    39601 aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagcaagcg ccagggcatg
    39661 gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc
    39721 agtaccgcca ccaccagacg caacagaaac gccatccagc caggttgcgg ggtaaagatt
    39781 ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata
    39841 gaaagtgaca gcccggcatg tttcaacgga ccaataaacg ccaggttcat caattgcgtt
    39901 aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag
    39961 ccaggagcca acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac
    40021 gcccgctggg tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg
    40081 gtcaacggac ctgaaagaat gcccaacgca accgcactcg gcagcgccaa caggaaacaa
    40141 agacgcagcc cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt
    40201 ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc ggacggaaac
    40261 tccattaagc ggtcggcgta atacatccac gacaccgaac cggaagcaag aaacgaggca
    40321 aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat tgccggtccc
    40381 atctgtttca ccacacgcat tgctccggca tcgtggaagt taatgcgtgg caaaaccagc
    40441 atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggatgcc gccgaccgta
    40501 acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa
    40561 ccaatcatgc tgatgttaag cagtgttgga gcaaacgccg gaatcgagaa gcggttccac
    40621 gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta
    40681 atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg
    40741 gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgcg
    40801 agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcatcttc accctgctta
    40861 cttttatatt ccgccagaat cggtacaaat gcctgggaaa atgccccttc ggcaaagata
    40921 cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg
    40981 ccaaagattc tggcgacaat tgcgtcacgt gcgaagccaa gcacacgcga aaacatggtc
    41041 atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac
    41101 gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg
    41161 aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc
    41221 aacgatacgt tgcgccagca ctgcctgttc gccggcggtt tgcggaactg tctgattttg
    41281 cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gcgtgctctg
    41341 ccaaccagga atcggtttat tcactacacc ctgcccacgc tcctcacgcc attcgcgcat
    41401 ttccgtaatg tcgatgagcg ccccgtcagt cacggcctgc acggtttcac gctgacttcc
    41461 ggcacggcga tgcatacaag tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc
    41521 aaacagcatt tcgccagcgt cgttagtcaa tagcgtaccg ccatccagag aggctttgcc
    41581 gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata
    41641 aagatcgtgt ggcccgacgc tattgctacg atgtttatcc attcttagcg aggctgcggt
    41701 ggcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat
    41761 caacgtcagt tttttccgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc
    41821 tgccagcggt ttatcgacac agacatgtac ccccgcattg agtaacgtac tgaccacgtc
    41881 aaagtgactg gcggtgctgg aatgtacaaa aaccgcatcg caactggcgg caaggctgga
    41941 taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt
    42001 aggcgaccag gctccttgta acgtccagtc agacgctgcc gccagcaccg gtaaccacgc
    42061 tttttgcgca atgccgccta atcccactac gccgatacgt aattttttca ctttaatctc
    42121 ccaggtgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg
    42181 cttcaacgcg ggcctgtaaa tcaccgtcaa cggcgctcga catatccgtt accgacggct
    42241 gatcctctac ctcaccgctg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc
    42301 gggccagacg caccacaaaa ggaccatctt cgcgattcgc cagttgttcc agcgtcaact
    42361 ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg
    42421 gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt
    42481 tcagatcgcc aaattctgaa ttacaaaaac gttgctcata tttggtgact cgattaccaa
    42541 agccactcac cgtgcgcaga taatgacgtt tgaccagatt atccagctgt tcctgcactt
    42601 cagattcact cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta
    42661 caccattgac tgagagcgga tattgctccg gcgtggtcac ctgcttttcc agcaagcagc
    42721 caatcacgcg ggcttccaga gcagtaagtt gatatttcat gagattctcc ttagcggccg
    42781 ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc
    42841 aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg
    42901 ctacgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc
    42961 atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc
    43021 ccaatcgaat aaccgagata gcaggcatga aaagagccgc gaacaacatt ggaaaaattg
    43081 gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct
    43141 tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg ataacagtgg
    43201 ctttcgtctc gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc
    43261 gcaagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag
    43321 cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg
    43381 tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa
    43441 atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg
    43501 ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagata
    43561 gtgctcatct tttatttccc gggaggggaa atgtcccgcg tgtcgcaggc gaggaacctg
    43621 ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc
    43681 ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt
    43741 gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg agcaattgcc
    43801 gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc
    43861 gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga
    43921 ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca
    43981 caacaacgtg gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt
    44041 ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg
    44101 gcagttttgt ttgtgctgtg tgcagcgttc aatgcgtggt tgttaccggc atggaaactc
    44161 tccaccgtac gcacgcccgt tcgcgaaggc atgacccgag tgatgcgcga caagcgtttt
    44221 gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg
    44281 ctgccaatta tggtcaacga cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc
    44341 attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat
    44401 tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg
    44461 gtgggcatgg tcagcggcct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg
    44521 tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct
    44581 cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggtggcgc tattggttat
    44641 atcggtggcg gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg
    44701 atgatgctgg gcattattgg catcttcact ttccttgcgc tgggttggca gtttagccag
    44761 aaacgcgccg cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc
    44821 ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct atatatttac
    44881 gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc
    44941 gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa
    45001 caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt
    45061 cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgcaatt
    45121 gaagagtggc tgcgcaaggt caatggctac gccaacaaac tgctgttgcc gcgttttgcc
    45181 aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa
    45241 gaggccagcg cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat
    45301 atcagcgatg atttacgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc
    45361 gaactttcgg aagatgatat tcagctattt ccactactgc gtaatctgac gctggtagcc
    45421 ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa
    45481 atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc
    45541 gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc
    45601 gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt
    45661 tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac
    45721 catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat
    45781 cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacctgacca gccaaattgg
    45841 tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct
    45901 gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa
    45961 agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga
    46021 aaaaatgcaa acggtgatgc aaacgttgct tccctatctg aaccaggcat tacgcaatta
    46081 ctttaaccag caacctgctt acgtcctgcg cgaagatggc agccagggcg aagcaatggc
    46141 gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga
    46201 ttaatcacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt
    46261 atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc
    46321 atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga
    46381 catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc
    46441 caatctggct ccgcccgtga ccaccgttga ggctgccgtg gcgtatcgcc aacgtattct
    46501 tgacgccgta cctgccgggc acgatttcac cccattgatg acctgttatt taacagattc
    46561 gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg ctgcaaaact
    46621 ttacccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat
    46681 gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac
    46741 acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc
    46801 tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc
    46861 tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct
    46921 gatgtttaac cgcaaccata tgctggttgg aggcgtgcgt ccgcacctgt attgtctacc
    46981 catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa
    47041 tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg
    47101 cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga
    47161 agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc cgcaattcta
    47221 tgggttgccg gtcaacgaca cattcatcga actggtacgt gaagagcatc aggttgctga
    47281 aagcatcgca ctgactgatg acacgctggt gccattcctc gccggggaaa cggtacgctg
    47341 gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt acctgtataa
    47401 ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt
    47461 ctccattgcc agctggggct attgacgccc tggctggcga actttctcgc cgtattcagt
    47521 atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat
    47581 cggttattgg cgcgacaaaa gaagataaac agcgcattag tgaaattctc caggaaacgt
    47641 gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg
    47701 ggtcgccccg gcttttttat tttacttacg aattttgagt aca
//
LOCUS       DAPGRN010000024        45712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPGRN010000024 DAPGRN010000000
VERSION     DAPGRN010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.024"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..675)
                     /locus_tag="RHW33_001497"
     CDS             complement(<1..675)
                     /locus_tag="RHW33_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV1645020.1"
                     /translation="MPQRILVLGASGYIGQHLVQALSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGESGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAV"
     gene            complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="RHW33_001498"
     CDS             complement(686..1687)
                     /gene="ltaE"
                     /locus_tag="RHW33_001498"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV1645021.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGIYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="RHW33_001499"
     CDS             complement(1724..3442)
                     /gene="poxB"
                     /locus_tag="RHW33_001499"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV1645022.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="RHW33_001500"
     CDS             complement(3575..4543)
                     /gene="hcr"
                     /locus_tag="RHW33_001500"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV1645023.1"
                     /translation="MTMPTNQCPWRMQVHHIKQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFITGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAQEFYAPVGTTLLEALESNKVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="RHW33_001501"
                     /gene_synonym="priS"
     CDS             complement(4555..6207)
                     /gene="hcp"
                     /locus_tag="RHW33_001501"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV1645024.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="RHW33_001502"
     CDS             complement(6351..7250)
                     /gene="lysO"
                     /locus_tag="RHW33_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV1645025.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7707..8402)
                     /gene="aqpZ"
                     /locus_tag="RHW33_001503"
     CDS             complement(7707..8402)
                     /gene="aqpZ"
                     /locus_tag="RHW33_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV1645026.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            8828..10486
                     /locus_tag="RHW33_001504"
     CDS             8828..10486
                     /locus_tag="RHW33_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV1645027.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDRRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10483..11439)
                     /locus_tag="RHW33_001505"
     CDS             complement(10483..11439)
                     /locus_tag="RHW33_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDV1645028.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11590..12705
                     /gene="macA"
                     /locus_tag="RHW33_001506"
     CDS             11590..12705
                     /gene="macA"
                     /locus_tag="RHW33_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV1645029.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12702..14648
                     /gene="macB"
                     /locus_tag="RHW33_001507"
     CDS             12702..14648
                     /gene="macB"
                     /locus_tag="RHW33_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV1645030.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14721..14945)
                     /gene="cspD"
                     /locus_tag="RHW33_001508"
     CDS             complement(14721..14945)
                     /gene="cspD"
                     /locus_tag="RHW33_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV1645031.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15268..15588
                     /gene="clpS"
                     /locus_tag="RHW33_001509"
     CDS             15268..15588
                     /gene="clpS"
                     /locus_tag="RHW33_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV1645032.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15619..17895
                     /gene="clpA"
                     /locus_tag="RHW33_001510"
     CDS             15619..17895
                     /gene="clpA"
                     /locus_tag="RHW33_001510"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV1645033.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18304..18391)
                     /locus_tag="RHW33_001511"
     tRNA            complement(18304..18391)
                     /locus_tag="RHW33_001511"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18355..18357),aa:Ser,seq:gga)
     gene            complement(18645..18863)
                     /gene="infA"
                     /locus_tag="RHW33_001512"
     CDS             complement(18645..18863)
                     /gene="infA"
                     /locus_tag="RHW33_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV1645034.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19148..19852)
                     /gene="aat"
                     /locus_tag="RHW33_001513"
     CDS             complement(19148..19852)
                     /gene="aat"
                     /locus_tag="RHW33_001513"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV1645035.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19894..21615)
                     /gene="cydC"
                     /locus_tag="RHW33_001514"
     CDS             complement(19894..21615)
                     /gene="cydC"
                     /locus_tag="RHW33_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV1645036.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLSRYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAVIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21616..23382)
                     /gene="cydD"
                     /locus_tag="RHW33_001515"
     CDS             complement(21616..23382)
                     /gene="cydD"
                     /locus_tag="RHW33_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV1645037.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23505..24470)
                     /gene="trxB"
                     /locus_tag="RHW33_001516"
     CDS             complement(23505..24470)
                     /gene="trxB"
                     /locus_tag="RHW33_001516"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV1645038.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            25015..25509
                     /gene="lrp"
                     /locus_tag="RHW33_001517"
     CDS             25015..25509
                     /gene="lrp"
                     /locus_tag="RHW33_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV1645039.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25644..29711
                     /gene="ftsK"
                     /locus_tag="RHW33_001518"
     CDS             25644..29711
                     /gene="ftsK"
                     /locus_tag="RHW33_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV1645040.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     DITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPEPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFTLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29866..30477
                     /gene="lolA"
                     /locus_tag="RHW33_001519"
     CDS             29866..30477
                     /gene="lolA"
                     /locus_tag="RHW33_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV1645041.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30488..31831
                     /gene="rarA"
                     /locus_tag="RHW33_001520"
                     /gene_synonym="mgsA"
     CDS             30488..31831
                     /gene="rarA"
                     /locus_tag="RHW33_001520"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV1645042.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31922..33214
                     /gene="serS"
                     /locus_tag="RHW33_001521"
     CDS             31922..33214
                     /gene="serS"
                     /locus_tag="RHW33_001521"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV1645043.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            33454..35898
                     /gene="dmsA"
                     /locus_tag="RHW33_001522"
     CDS             33454..35898
                     /gene="dmsA"
                     /locus_tag="RHW33_001522"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV1645044.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLLDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGIHNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            35909..36526
                     /gene="dmsB"
                     /locus_tag="RHW33_001523"
     CDS             35909..36526
                     /gene="dmsB"
                     /locus_tag="RHW33_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV1645045.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            36528..37391
                     /gene="dmsC"
                     /locus_tag="RHW33_001524"
     CDS             36528..37391
                     /gene="dmsC"
                     /locus_tag="RHW33_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV1645046.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(37427..38053)
                     /locus_tag="RHW33_001525"
     CDS             complement(37427..38053)
                     /locus_tag="RHW33_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1645047.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCLAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            38368..39516
                     /locus_tag="RHW33_001526"
     CDS             38368..39516
                     /locus_tag="RHW33_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645048.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            39726..41156
                     /locus_tag="RHW33_001527"
     CDS             39726..41156
                     /locus_tag="RHW33_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1645049.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(41157..41267)
                     /locus_tag="RHW33_001528"
                     /pseudo
     CDS             complement(41157..41267)
                     /locus_tag="RHW33_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(41366..42106)
                     /gene="pflA"
                     /locus_tag="RHW33_001529"
     CDS             complement(41366..42106)
                     /gene="pflA"
                     /locus_tag="RHW33_001529"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV1645050.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(42298..44580)
                     /gene="pflB"
                     /locus_tag="RHW33_001530"
     CDS             complement(42298..44580)
                     /gene="pflB"
                     /locus_tag="RHW33_001530"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV1645051.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(44635..45492)
                     /gene="focA"
                     /locus_tag="RHW33_001531"
     CDS             complement(44635..45492)
                     /gene="focA"
                     /locus_tag="RHW33_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV1645052.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        11247 a        11415 c        12114 g        10936 t
ORIGIN      
        1 caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc cggcggcttc
       61 gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc accagatagt gcagcaagtt
      121 ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta acactggcag
      181 gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc
      241 ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga atgtccgccg tagcctgacg
      301 cgcgcgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga gaaagattaa
      361 ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct ggcgctcctg
      421 agcgataaaa tcgccgcttt cgcccatgct gtgcaccaga aaatagacgg tatcgatatc
      481 ctgtaacagg gccggaaggt tatccggcca gctgagatcg actttatggc aactgacgtt
      541 tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga tctgatgccc
      601 ttgctggctg agtgcctgca ccagatgttg accaatgtag ccactggcac caagaactaa
      661 aatgcgttgc ggcacgtctc cctccttaac gcgccaggaa tgcgcgccag tgggcggcga
      721 cttctgccag ttgttcgcgc gagacatcaa gatgcgtcac caggcggaca atcggcgagg
      781 cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca ttttcttcgc
      841 cgacgcggac aaacagcata ttagtgtcct gacgcattac atccgcgcct gcttcacgca
      901 gctgctccgc catccaggcg gcgttgtcgt gatcttcctg caagcgtgcg acgttatttt
      961 tcagggcata tatcccggcg gcagccagaa tgccggactg gcgcatcccg ccacctgtca
     1021 ttttccgcca gcgaatggca cgtttaatgt aatcacgatt accgacgagt aatgaaccga
     1081 ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat tgcgtgatct
     1141 ctttcagttc gcagccgtaa gccaccacgg cgttaaagat gcgcgcaccg tcaacatgca
     1201 gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca cgcggcaaca
     1261 ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa tggatatcgt
     1321 cgggtttgat tttcatcgcc actttatcca gcggcagcgt gccgtcggca gccgcgtcga
     1381 tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac agatagttat
     1441 gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc agagcgacca
     1501 ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa agctctgctg
     1561 catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg
     1621 cggccatcat cgcttcgagc atggcgcggc tcggtcgggt tacggtatca ctgcgtaaat
     1681 caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt agccagttcg
     1741 ttttcgccag ttcgatcact tcatcgccgc gtccgctgat gattgcgcgc agcatataca
     1801 ggctgaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac tcttctttgg
     1861 cgaccaccac atccaccaac accggaccgt cgatggagaa ggcgcgttgc agggcttcat
     1921 cgatttcaga ggctttttct acacgaatac ccgtaattcc gcacgcttcg gcaatgcggg
     1981 caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta gcctccggct ttcatctcca
     2041 tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcaccggc agtttcatct
     2101 gcactaccga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg cacatggcga
     2161 ccacctgacg ttccggctcg gttgcctgcg cgcccagcgc ctgcggcatg gcgttagcca
     2221 tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttc agataacgcg
     2281 ctgcccacac cgttggcgta ccaacgtcac aggtgaaaat ggcgtcatcg gcggcaaaat
     2341 gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt ttagctaaat
     2401 cgtccagccc tttgcgggca tcgcggtaat cttccagcgc tttatccaga aatttgcgat
     2461 cggctttttc ttccaccagt ggcagcaatg cacgcagggt tgatttgata tcgccgacca
     2521 gtgccatatc caccttgctg tgagcgccga tgctggctgg attgatatca atctgaataa
     2581 ttttggcatc ggtcgggtag aacgcacggt aggggaactg agtaccgagt agcactaacg
     2641 tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg gtcattccaa
     2701 catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga acaataggtg
     2761 ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag ccgctgccac
     2821 acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt aactcttctt
     2881 cttccggcgt cacgaccggc tgtggcgcat gataccagtg ggtagttgcc ccttctggcg
     2941 caggttttaa cgccacgtcg cccggtaaca cgacaacgga aacgccacgg ttaagcaccg
     3001 ctttgcgcat ggcaatcgcc agcacttgtg ggatctgctc cgggctggaa accagctcgc
     3061 aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc
     3121 ctatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc
     3181 aatcgaacag gccgttgatc aagtgcaggt tgcctgggcc gcacgatccg gcacagaccg
     3241 ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact tcttcatggc
     3301 gggtggacat ccactcgatg gtgcccatgc gattaagact gtcgctaaga ccgttcagag
     3361 agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt ttggcgatat
     3421 aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt
     3481 tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac ggttaaatag
     3541 cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa tccccctgcg
     3601 gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg
     3661 tactgctcac cgtatattca ccggaaacca ctttcgtctt acagcaaccg caaacacccg
     3721 cgcggcaggc ggcgacaacc ggaactttat tgctttccag cgcctccagc agcgtggtgc
     3781 caaccggagc gtaaaattct tgcgccggtt gcagtttggt gaatttcaga ccgctggtcg
     3841 ccgcttccgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt
     3901 tcacttcctg ctctacccaa tccatatacg gagccgggcc acaggtcatc acggtacgtg
     3961 aagctaagtc aggaacgcga gtcaggagtt cgcgagtgag acgaccagtg ataaagcctt
     4021 cggtaacgtt attttccgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa
     4081 taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt ggacggttct
     4141 tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca
     4201 ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga
     4261 gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc
     4321 gccgcacggt cagggtgata tattcactca cacctggcgt ggaggaaata gtgtaagcac
     4381 gcagcgtttc cgctgagtta cgcacgctga ccagtgcata ttgcccggcg cgatatgggt
     4441 agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc ttaatgtgat
     4501 gaacctgcat ccgccacggg cattgattcg ttggcatcgt catctgctaa ctccttacgc
     4561 gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc cgaatttctc
     4621 gttcagcacc gccagcagat ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat
     4681 atttttcacg cccagagaaa gcagcgtcag cagaatgaca atcgctttct gttcaaacca
     4741 ggagagcacc agcgacagcg gcagatcgtt gacaccgcag cccagttttt ctgccagcgt
     4801 aaccgccaga ataattgctg agtaagcatc gttacactga ccggcatcca ccagacgagg
     4861 cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac aggcgagagt
     4921 caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt gacgttcgcc
     4981 gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac ggctcaccag
     5041 atcaatcagc gtatccgctg cgccaagcag cgtctggcgg ccaaagccca cggtgatcag
     5101 gtgcggaatt tcgctgtatg ggaagcctgc catttgttgc gcctgggcaa tgaccgcaga
     5161 gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc gggtccagat
     5221 acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg
     5281 gccagggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt agttaccgac
     5341 cagatgcttg aatttacgca gctccgggta gccgtgggca ggcagcattt cgccgtgggt
     5401 gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc
     5461 gtgaccggaa atcaggatgc atttacccgc cgtcgctttg acgttgacct gggttggcgt
     5521 cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt tgaagttcat
     5581 ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag
     5641 ccacgccatg attttatgat actgggcata aatatcgttg tcgtattgac cgagaacgtg
     5701 tgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc
     5761 gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg
     5821 ttgcagttcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg
     5881 ggcgttggca tctacagcca ggcattgtgc tttaagcgcc tcgcgcaagg caatcgcttc
     5941 acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa
     6001 ggcgcgtggc gcgaagctgt cgacatcgtg gttgatgatg ccgtattcac gcgcttttac
     6061 cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaggtttc
     6121 cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg gagtacggat
     6181 agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca
     6241 tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca actttaaggg
     6301 agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta ttacgcagag
     6361 aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac aattgccgcc
     6421 gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc catcgacgtc
     6481 gcaccgcata agcccagtgc ggtagagcgg ctgcggcgaa tcagcccagg gatcaacata
     6541 attgcaatca gctcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca
     6601 aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccaga ggccattgcc
     6661 agcgcggtat tgatgggtag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa
     6721 ctggcaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag
     6781 gtcatgccat tattgcgcaa ctgaataccg acgaggaaaa gtagcagaat taaggtgtat
     6841 tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcaggccaat ggcaaaacca
     6901 atcactacta cgccgcacag ttttagcgac tcaagcgcca tcgcaatacg cgacgggagt
     6961 ttttcttgct gatggtggtt gcgccacggc agaccgcgct ccagccacat aagggcggca
     7021 atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg
     7081 ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa
     7141 accatccagc ttaatagctg attaataact cttaatgcag ctttttggcg aagcggaatg
     7201 aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaacccag
     7261 cccttgcaaa gaaagaagtc gaaatgcgcg atgacacact actgaaagcg gaaggacgag
     7321 taaagttgca attaaaagga aatgttatgc atcagagggg gatttttaat tctatatgag
     7381 tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact ggcgcaggga
     7441 gcgcgcaggg ggcggccagt cgccgccgcc ccctgtcccc cgggctctgg cgaaaaaaat
     7501 cgccgctgcg cggtaccttc agcttatccc cttctgctac cgggtcgggc gcgaggtaac
     7561 gtcctgtaag acgcgccctc agcccacatc catgtgggct gccccggcct tcggggaacg
     7621 cttcagcgat tttgacgcca ccaacacccg tgcggtttgt aacgtcgaag ataaaacaac
     7681 cagggcagat aaaatgccaa tgctaattaa ttacgttttt ccagcagggt ccggtaaatc
     7741 agaccaccga taatgccgcc gacaattggc accacccaga agaaccacag ttgttctaat
     7801 gcccagccgc cctggaagat ggcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta
     7861 gtcaccggaa tactgattaa gtgaatcagg gttaaggcca gaccaatagc gatcggcgca
     7921 aaacctgccg gagcgaattt gtcggttgcg ccgtggatca ccaacaggaa acctgcactc
     7981 aataccagtt caactaccag cgcggaaagc atggaataac cgcctggtga atgctcgcca
     8041 taaccgttag aagcaaaacc gctggctgcc gcgtcaaaac ccgttttacc actggcaatt
     8101 aaatacagca gcgccgctgc aacaataccg ccgacaacct gggcaattac gtagccaacg
     8161 acttcttttg ccggaaaacg tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa
     8221 tgaccaccag aaatatgacc aacagcgaag gccatcgtca gaacggtcaa accgaatgcc
     8281 agcgccacgc cggcaaaacc aatgcctaat tccgggaagc ctgcggccag tacagcacta
     8341 ccacagccac caaaaacaag ccagaaagta ccaaaacatt cagctgctaa ttttctgaac
     8401 atatccacct caattaaaaa ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg
     8461 taaaaaagtg acgacggaaa taatgcgcgg ctattttaaa aacgaaggcg agtcattcac
     8521 cagataaata aatccagtaa atttgattta gggcaacagc gggttgcccc atatagtcat
     8581 ttgtctgatt gacagtgtag tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat
     8641 aatttatcat gctaatcatt tgattttttt gtttttgcag acttatcagc aagagggagt
     8701 ataacgcgat tattcgctca tttttcagac atttgccatg cttaaatgtg atgtcatcac
     8761 gtattagcaa ggcctttccc gttatactgc cagcgtaaag gataagtcac atatttctgg
     8821 aggggatatg attcttgagc gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc
     8881 gttgatgctg gaacaaaaca acgtcctgat tggggagaac gcgtggggta aatccagctt
     8941 gctggacgcc ttaaccctgc tgctatcgcc agaatcagat ctctaccatt ttgagcgcga
     9001 cgatttctgg ttcccgccgg gagatatcaa cggtcgggaa catcatctgc atattatttt
     9061 gaccttccgc gaatcgctgc caggccgaca tcgggttcgc cgttatcggc cgctggaagc
     9121 gtgctggacg ccatgcaccg atggctatca ccgtattttt tatcgtctgg aaggggagag
     9181 tgcggaagac ggcagcgtga tgacactgcg cagttttctc gataaagacg gacatccgat
     9241 tgatgttgag gatattaacg atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg
     9301 cttgcgtgat gcccgtttta tgcgccgtat tcgtaacggc acggtgccaa atgtccccaa
     9361 tgtggaagtc accgcgcgcc agctcgattt cctcgcccgt gagttgtcct cacatccgca
     9421 aaatctctct gatggacaga ttcgtcaggg actttccgca atggtgcagt tgcttgagca
     9481 ttatttctct gagcaggggg ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag
     9541 caatgagcaa cgaagctggc gctatctgga catcatcaac cggatgattg accgacctgg
     9601 agggcgctcg tatcgggtta ttttgctcgg cctgtttgct actttattgc aggcaaaagg
     9661 cacattgcga ctggataaag acgcccgtcc attgttgctg atcgaagatc cagaaacgcg
     9721 tttacacccc attatgcttt cagttgcctg gcatctgttg aatcttttgc ctttacaacg
     9781 tattgctacc accaactctg gcgagctgct ttcgttaact ccagtggaac atgtttgccg
     9841 cctggtgcgt gaatcttctc gtgttgccgc ctggcgtctg ggaccaagtg gcttgagcac
     9901 cgaagacagg cgccgcatct cttttcacat tcgttttaac cgtccgtcat cgctgtttgc
     9961 tcgctgctgg ctgctggtgg aaggggaaac ggaaacctgg gttatcaatg aactggcgcg
    10021 tcagtgcgga catcattttg atgccgaagg aatcaaggtc attgagtttg cccagtccgg
    10081 gctaaagcca ctggttaaat ttgcccggcg aatggggatt gaatggcatg tactggtcga
    10141 tggcgatgaa gcagggaaga aatatgccgc tacggtacgc agcctgttga ataatgatcg
    10201 ggaagccgaa cgagaacatt taacggcgtt accggcgctg gatatggaac attttatgta
    10261 tcgccaggga ttttccgatg tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat
    10321 gaatctgcgc aaaattatct cgaaagcgat ccatcgctct tccaaacccg atcttgccat
    10381 tgaagtggca atggaggccg gacgtcgtgg tgtggactcc gtaccgacgc tgctgaaaaa
    10441 aatgttctca cgcgtgctgt ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt
    10501 ggccatttgt ggctgaatag cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt
    10561 tttactggcg atttcggcaa tctcttttcg agctatctgt gctggaaggc ggtaatggcg
    10621 ttcagcatca catacgccgc caaccgattc ccagaaagcg ttgtaatcgg catggatctt
    10681 gccttcttta tcgcgataac gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat
    10741 aatctgctct acctgcaaac gttgggcaaa cagacaggcc gcttccatca cgaggcgttt
    10801 gggaaatagc ccgtggcagg ctttcgtcgc attctggatt tcctgatgtg gaatttccca
    10861 ttttgcgcct tgcaatccac caataaacat cgttcttttc ccctgatatt cacacagggt
    10921 aaacgtgatt tctgccagag gaataccttc gctgttgcgg aacaggattg tgctatcacc
    10981 ttctttatcc attgagatca tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc
    11041 ttccagcttc gccagttgca gcccgggggt attcaaatat aagctgaatt cttccgccga
    11101 catacacccg cggagtaacg cataatggta acgtaacgcc tccagcaatt gcttacggct
    11161 aagattcgcc gcaaggtaag ggcgatgcag acgtacaggc agtcgcggct ggcgcgttaa
    11221 caacacattg agattaggcc agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg
    11281 cataatcaac gagcgcagca aaaatttctg gcgaaaacta cggcgatgcc agaatttacc
    11341 cggccgacac tgtccacgtg ccagactaag aaaaagtgac aggctgctga gagattcaga
    11401 tgacgtaaag gtccgttcag ttagctgcga catattcatg aaatcaatgg ttatacatga
    11461 cgtcgatttc accattgcgt atcttaacca aacatcaata gtgtgattac taacgtaaat
    11521 tttagggttt tgttgatatt tcgttgaagt taatgacccg gattggcata tggagtattc
    11581 agaatattta tgaaaaagcg gaaaaccgtg aagaagcgtt acgttattgc gctggtgata
    11641 gtcatcgccg gactgattac gttatggaga attcttaacg cacccgtgcc gacttatcag
    11701 acactgattg tgcgccccgg tgatttacag caaagcgtgc tggcgaccgg aaagctggac
    11761 gcgctgcgta aggttgacgt gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg
    11821 gcgattggcg ataaagtaaa aaaagaccag cttttagggg ttattgatcc tgaacaggct
    11881 gaaaaccaga tcaaggaggt cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag
    11941 gcggaagcgg agctgaaact ggcgcgtgtg acgtattccc gtcagcaacg tctggcacaa
    12001 acgcaggcgg tttcacagca ggatctcgac accgccgcga cggagatggc tgtgaaacag
    12061 gcgcaaattg gcaccattga cgcgcaaatc aagcgcaatc aggcttctct cgatacggct
    12121 aaaaccaatc tcgattacac gcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc
    12181 accactctgc aaggccagac ggtgattgcc gcacaacaag caccgaacat tctgacgctg
    12241 gcagatatga gcaccatgct ggtaaaagcg caggtttctg aagcggatgt aatccacctg
    12301 aagccggggc aaaaagcctg gtttacggta cttggcgatc cactgactcg ctacgagggg
    12361 caaatcaagg atgtactacc gacgccggaa aaggttaacg acgctatttt ctattacgcc
    12421 cgttttgaag tccccaaccc caatggtttg ctgcggctgg atatgactgc gcaagtgcat
    12481 attcagctca ccgatgtgaa aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg
    12541 gttggcgata atcgttataa agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa
    12601 gtgacgattg gcgcacgtaa cgataccgat gttgagattg tcaaagggct ggaagcgggc
    12661 gatgaagtgg tgattggtga ggccaaacca ggagctgcac aatgacgcct ttgctcgaat
    12721 taaaggatat tcgtcgcagc tatcctgccg gtgatgagca ggttgaggtg ctgaagggca
    12781 tcaccctcga tatttatgct ggtgagatgg tggcgattgt tggcgcttcg ggttccggta
    12841 aatcgaccct gatgaatatt ctcggctgtc tggataaggc caccagcggc acctatcgcg
    12901 tcgccggtca ggatgttgcc acactggacg ccgatgcgct ggcgcaacta cgccgcgagc
    12961 atttcggctt tattttccag cgttaccatt tgctttcgca tttaaccgcc gagcagaacg
    13021 ttgaagtacc cgccgtctat gctggtcttg agcggaaaca gcggctgctt cgcgcccagg
    13081 agttgctgca acgactgggg ctggaagacc gtacagagta ttatccggca cagctttcgg
    13141 gtggtcagca acagcgcgtt agcatcgcgc gggcattgat gaacggcggt caggtaattc
    13201 ttgccgatga accaaccggc gcactggaca gccattctgg cgaagaggtg atggcgatcc
    13261 tgcatcagct gcgcgatcgc ggacatacgg tgattatcgt cacccacgat ccgcaggtcg
    13321 ctgctcaggc cgagcgggtg atcgaaattc gcgacggcga aattgtgcgc aatcctcccg
    13381 ccattgaaaa agtgaatgtt gctggcggga cggaacctgt tgtcaacacg gtgtctggct
    13441 ggcggcagtt tgtcagcggt tttaacgagg cgctgacgat ggcatggcgg gcgctggcag
    13501 cgaataaaat gcgtacttta ctgaccatgc tggggattat tatcggtatt gcgtcggtgg
    13561 tttccattgt cgtggtaggt gacgccgcca aacaaatggt gctggcggat attcgttcta
    13621 ttggtacgaa tactattgac gtctaccccg ggaaagattt tggcgatgac gatccgcaat
    13681 atcagcaggc gctgaagtac gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct
    13741 cagccacacc tgccgtctcg caaaacctgc gcctgcgtta taacaatgtt gatgttgctg
    13801 ccagtgccaa tggcgtgagc ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag
    13861 ggaacacctt taatcaggag cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta
    13921 atactcgccg ccagcttttc ccccataaag cagatgtggt tggcgaggtg attctggtcg
    13981 gcaatatgcc cgccagagtc attggtgtgg cggaagaaaa acagtcgatg tttggtagca
    14041 gtaaagtgct gcgtgtctgg ctaccttaca gcacgatgtc agggcgagtt atgggccagt
    14101 cgtggcttaa ctccattact gtcagggtga aagaaggatt tgacagcgcc gaggcggaac
    14161 agcaactcac gcgtttactt tcactgcgcc acggtaagaa ggatttcttt acctggaaca
    14221 tggacggcgt cttgaaaacc gttgaaaaga ccacacgtac tttacaactg tttctgacgc
    14281 tggtggcggt gatttcgctg gtggtgggcg gtattggtgt aatgaatatt atgctggtgt
    14341 cagtgactga gcggacgcgg gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg
    14401 atgttttgca acagttcctg atcgaagccg tactggtttg cctggtcggt ggcgcgttgg
    14461 gaataacact gtcactgtta attgctttca ccttgcagct tttcttaccc ggctgggaga
    14521 ttggtttttc accgttggcg ctgctgctgg cgtttctctg ctcgacggtc accgggattt
    14581 tatttggctg gttacccgca cgaaatgcgg cacgactgga tccagtagat gctctggcac
    14641 gagagtaatt tttgagataa aaatgccagc cgatcgggct ggcattttgc ctttaggatg
    14701 tacacaatga gacagaagag ctatgcgact gccgcttcta cttcgacggg cacaataaca
    14761 ctggcgtgat tgccttttgg cccctggtgg acatcaaact gaacggattg tccagctttt
    14821 agcgttctgt aaccatccat ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg
    14881 ccttcagggc agatgaaacc aaaccctttg gcattgttga accacttaac agtacccttt
    14941 tccatgcttc gacatccttc gcaaatctta tacaagtaag atggaataaa ccggggtcag
    15001 agagggggct gttcaaaacc tcgccaactc tagaaataca atttagagaa ttagggcgag
    15061 ccgtcaagca tttgacaggg gccagggggc aggtatgaat caaaaatttg aagcagttaa
    15121 cgctattgac aggaatgtga cagatgtcgc tgatgccaac gatagatgat agttatctat
    15181 catgtggagt agattggtca ggcaaataag ctcttgtcag cggcagggcg ttctgccgat
    15241 aaccgtaacc gaagatgata actgacaatg ggtaaaacga acgactggct ggactttgat
    15301 caactggcgg aagaaaaagt tcgcgacgcg ctaaaaccgc catctatgta taaagtgata
    15361 ttagtcaatg atgattacac tccgatggag tttgttattg acgtgttaca aaaattcttt
    15421 tcttatgatg tagaacgtgc aacgcaattg atgctcgctg ttcactacca ggggaaggcc
    15481 atttgcggag tctttaccgc cgaggttgca gaaaccaaag tggcgatggt gaacaagtac
    15541 gcgagggaga atgagcatcc attgctgtgt acgctagaaa aagcctgaat gcaggcataa
    15601 aaattggggg aggtgcctat gctcaatcaa gaactggaac tcagtttaaa tatggctttc
    15661 gccagagcgc gcgagcaccg tcatgagttt atgaccgtcg agcacttgtt actggcgctg
    15721 ctcagtaacc catctgcccg ggaggcgctg gaagcgtgtt ctgtggattt ggttgcgctc
    15781 cgtcaggaac tggaagcctt tattgaacaa accacacccg ttctgcctgc cagtgaagag
    15841 gagcgcgaca cacagccgac gctgagtttt cagcgtgtac tgcaacgtgc ggtcttccat
    15901 gtccagtcct ccggtcgcaa tgaggttacc ggtgcaaacg ttctggtcgc tatctttagc
    15961 gaacaggagt cgcaggcggc atatctgttg cgtaaacatg aagtcagccg tctcgatgtg
    16021 gtgaacttta tctctcatgg cacgcgtaaa gacgagccga cacagtcttc tgatcctggc
    16081 agccagccaa acagcgaaga acaagctggt ggggaggaac gtatggagaa tttcacgacg
    16141 aacctgaatc agcttgcgcg cgtgggcgga atcgacccac tgattggtcg tgagaaggag
    16201 ctggagcgtg ctattcaggt tctctgccgt cgccgtaaaa acaacccgct gctagtgggg
    16261 gaatctggtg tcggtaaaac cgcgattgca gaaggtcttg cctggcgaat tgttcagggc
    16321 gatgtgccgg aagtgatggc tgactgtacg atttactctc tcgatatcgg ttctctgtta
    16381 gcgggcacaa aatatcgcgg cgactttgaa aaacgtttta aagcgttgct caagcagctg
    16441 gagcaggaca ctaacagcat cctgtttatt gatgagatcc acaccattat cggtgcgggt
    16501 gcagcgtctg gtggtcaggt cgatgcggct aacctaatca aaccgttgct ctccagcggt
    16561 aaaattcgcg taattggttc gacaacctat caggagttca gcaacatttt cgagaaagac
    16621 cgtgctctgg cgcgtcgctt ccagaaaatt gatattactg aaccgtcgat cgaagaaact
    16681 gttcaaatca tcaatggcct gaaaccgaag tatgaagcgc accacgacgt gcgttatacc
    16741 gcaaaagcgg tgcgtgcggc ggtagagctg gcggtgaaat acattaacga tcgtcatctg
    16801 ccggataaag cgattgatgt tatcgacgaa gcgggcgctc gcgcacgcct gatgccggta
    16861 agcaaacgca agaaaaccgt taatgtggcg gatattgagt ccgtggtggc ccgtattgca
    16921 cgcattccag agaagagtgt ttctcagagt gaccgcgata ccctgaaaaa cctcggcgat
    16981 cgcctgaaaa tgctggtctt cggtcaggat aaagccattg aggcgctgac tgaagccatt
    17041 aagatggcgc gtgcaggttt aggtcacgaa cataaaccgg tcggttcgtt cctgtttgcc
    17101 ggtcctaccg gggtcgggaa aacagaggtg acggtacagc tttcgaaagc tttgggcatt
    17161 gagcttctgc gctttgatat gtccgagtat atggaacgcc ataccgtcag ccgtctgatt
    17221 ggtgcgcctc cgggatacgt tggttttgat cagggcggtt tgctgactga tgcggtcatc
    17281 aagcatccac atgcggtgct gctgctggac gaaatcgaga aagcgcatcc ggacgtgttc
    17341 aatattctgt tgcaggtgat ggacaacggt acgctgaccg ataacaacgg acgcaaagcg
    17401 gacttccgta acgtggtact ggtgatgacc actaacgccg gggtacggga aactgagcgc
    17461 aaatccattg gtcttatcca ccaggataac agcaccgatg cgatggaaga gatcaagaag
    17521 atctttacac cggaattccg taaccgtctc gacaacatta tctggttcga tcatctgtca
    17581 actgacgtga tccatcaggt ggtggataaa ttcatcgtcg agttgcaggt tcagctggat
    17641 cagaaaggtg tttctctgga agtgagccag gaagcgcgta actggctggc cgagaaaggt
    17701 tacgaccggg caatgggcgc acgtccgatg gcgcgtgtca tccaggacaa cctgaaaaaa
    17761 ccgcttgcca acgaactgct gtttggttcg ctggtggacg gcggtcaggt caccgtcgcg
    17821 ctggataaag agaaaaatga gctgacttac ggattccaga gtgcacaaaa gcacaaggcg
    17881 gaagcagcgc attaatctga ttgttaagta ggttggtgaa gtccgtaatc tcgaaagagg
    17941 ttacggactt tttatttatg gggtggaacg gtgaaaaccc tatttttgga ggtgaaggta
    18001 agttgttgat aattagtgct gctggaaaag gtaaggataa aaaagggtga cagcaggaaa
    18061 atgagatggt tttgctttat taacaacggg ctaaacgtgt agtatttgag ttcactgccg
    18121 tacaggcagc ttagaaaaac ctaccgtctt ggctagcggt tgcagcgaac gttcactgcc
    18181 gtacaggcag cttagaaatt cactggtaac atactccacc cgcccaccat gttcactgcc
    18241 gtacaggcag ataaaatgcg aaaaaaaaaa gctcgcactt tcgtacgagc tcttctttaa
    18301 atatggcggt gaggggggga ttcgaacccc cgatacgttg ccgtatacac actttccagg
    18361 cgtgctcctt cagccactcg gacacctcac caaattgttt tgttgcctga cctcatgggg
    18421 ggcaacgggg cgctactata gggagttgga gtaaaacggt caagaagaat tttaatgata
    18481 attattgttt gctcatactg taaacaagtt gtgcagtata tctacatcga gacaagttat
    18541 ggacttatac ttccaaagta cttcctacat atcacaaaat aaaaaggccg gttaaaccga
    18601 ccttttactc gttctttctc ttcgcccatc aggcggtaaa acaatcagcg actacggaag
    18661 acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa cagtcacttt gtcgcccgtc
    18721 aggatgcgga tgtagttttt gcgcatttta ccggagatgt gtgcagtaac cacgtgaccg
    18781 ttttctaact ctacgcggaa catggtatta ggcaacgttt caagaacggt accttgcatt
    18841 tcaatattgt cttctttggc catctaatcc tctggggtat cactaccgta atttgaaccg
    18901 gcaagataat gccgaagttc tgtaaataag taaagatttg cgcgctaaat cgcaacaaac
    18961 aggttcggca cattactccg aaaacacacg gctaagccgc accaaaagcg caacgtataa
    19021 gggagcggtg agataaacga tgggcgttac ctgacgcgaa aaattcctta tcggcagcgg
    19081 ggtaatgagc gtaaccaact ctgcgaccgc aattataaca ctctggggag aaatgtgccg
    19141 aaaacattca ttcttgtggt gaaaacaagc accgtggtac ccagaaatta ttcggcaatc
    19201 gtccgaggcg catttgattg agataattaa ggtaatcccg gcggggaatt tcgcaggcac
    19261 caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat aagcttaccg ccatgaccga
    19321 taaattcctc acagaatacc agaagcgccg ttttagacgc attttccatc cggctgaaca
    19381 tggactcgcc acaaaatagc gttccctggg ccacgccgta cataccgccg acaagctcat
    19441 cttcacgcca gacttcaatg gagtgggcat gaccgagttc gtgaaggcga tggtaggctt
    19501 cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc gctggcacag ccttcaatga
    19561 cctgaccaaa agcgtaattc atcgtgacac gatagggcga gcgtttatga aatcgcttca
    19621 tactacggct gatatgcagt gattctggcc atagcaccgc gcggggatcg ggcgaccacc
    19681 agaggatggg gtcgcctgga gaaaaccacg gaaaaatacc acgctggtaa gccattaaca
    19741 ggcgcgcagg gctaagatcg cccccaagtg ccagcaggcc gttaggctca cgtaatgcgc
    19801 cttccgggga agggaaggct attgaatggc gagaaagctg aaccaggcgc atgaccgcaa
    19861 aactccacgc aagtcggatc gttcaataat agcttacaaa ccctgcttga actggtaata
    19921 acgcccctgt ctggcaagca gttctgcgtg agtaccttgc tcaataattt gcccgttgtc
    19981 catcactatt atttgttgga aacgagagag tccgcgaagt cgatgggtga ccattaacac
    20041 cgttttctca cgcatcattt ctgcaagcaa ttcaaggatc tggctttcgg ttgtggcatc
    20101 taagccttcg gtaggttcat ccagcaacac cagtggcgca tcatgtaaca gcgcacgggc
    20161 gatagccaga cggcgcagtt caccaccgga gagctggcgt ccgccttcac ctaaccaact
    20221 gttgagacct gcatcctcga gcagcttctc cagaccaacg cgacgtaaga tcaccgccag
    20281 agcctcatca ctactgccag gcgaggcgag taacagatta tcacgcagcg tggcgctaaa
    20341 cagatgcact cgctgaggaa caacgctgat ggtctgtcgt agagccgctt cattcaggct
    20401 ggctatgggg ctatcgttaa gcaaaatctc gccctgttgc gggtcccatg cgcgggtcag
    20461 cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg agaatcgcaa tatgttcccc
    20521 ggcgtttacc tgaagagaaa tccctttaag tgcctgttga gattgctccg gataagtgaa
    20581 ctgaacatcc cgtaacgtca gcgaaacgcg atcggcaaca cgagtttggg tatcagggaa
    20641 ggtgacctcc ggtttttgat ccgttaaatc agtgatccga acggcagagg caatgacttg
    20701 ccccagatgc tgaaatgcac ccgttactgg tgccagtgct tcaaacgcgg ctaacgcgca
    20761 gaagacaaac agggcaatta acgcgccggg ttgagcattg ccgccaacgc cgccagacgc
    20821 catccacagc atcaggatca ccgctaacgc gccaatgagc agcattatcg cttgcgacaa
    20881 tgcggtcagt tcagattgac ggcgttgcgc ttccagccat tgaatttctg tattctctag
    20941 ttgcgtgcga taacgatcgc tggcaccaaa aatggtaagc tcagcttgcc cttgcagcca
    21001 ggccgtcagt tgttggcgat actgtccgcg aagatgagtc agattttgcc cggtgctttt
    21061 tcccgcacga taaaacagcg gtggcatcag gaaaagcgtc agtaacataa tgccgcccag
    21121 cgtaaaggcg agggtgaaat caaggaaact taacccgatt gtcaccacca taatcaccac
    21181 aaaagcgccc accagcggcg agataacgcg caggtaaaga tgatcgagcg tatcaacatc
    21241 cgccaccacg cggttgagca attcgccctg acgatagcgc gacagtccgg caggggagag
    21301 gggcaacaat ttgctgaagg tgtaaatgcg cagatgctgc aacacgcgga aagtcgcgtc
    21361 gtgacttacc agacgttcaa aataacgccc ggcagtacgg gtgattgctg caccacgcac
    21421 gcccgcagcg ggtagcatat agttgaagct gtacagtccg gcaacccccg caaccgctga
    21481 ggccgagagg aaccagccgg aaagtgtcaa cagaccgata ctggcgagca gcgtcacaat
    21541 tgccagcaca ataccaagac ttaacatcca tttatgacgt ttatacagtg ccagataggg
    21601 tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc agtaatgtgg cgaatgggcc
    21661 accagccaca cttaattccg cgtaacgtcc ttgctcaata atccggccat cctgcataac
    21721 ccaaatgaca tcccagtcag caagatcttc taactggtgg gtgaccatta acgttgtctg
    21781 gcgcagagag gcggcattca gcgcctccat tacgcgctgt tcactgtgag catcaaggct
    21841 ggcagcgggt tcatccaaca gtaatagcga acagggattt agtaacgcac gggccaccgc
    21901 cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg tcgccaacag gcgtatcaac
    21961 gccttgtggc aggagcggta gaaactcgct gacccaggcg ttatccagcg ctgcttgtaa
    22021 ttcttgttcg ctggcatcag gtcgcgccag tagtacgtta tcccgcaatg ttgctgccgg
    22081 taattgtggg ttttgcccaa cccaggagag atgtttacgc catgattctg gtgataaatc
    22141 gcgtaattct atcccgttga ttcgtaatga tccctgatat gagagaaaac cagaaagcgc
    22201 gttcagcagt gagcttttac ctgaaccgct gcgaccaacc aacactgcac gttggcctgc
    22261 tggcaaagta aagttcagcg gtccggccag cgttttacct tccggcgacg tgataaacag
    22321 atcctcggcc tcaatggtca ccggatcggt cgatgctaat tccgcctcac cgcgctgcgg
    22381 atgggcgagc ggggtttcca taaacgtttt cagactgtca gctgcgccaa cagcctgggc
    22441 tttagcatga taaaacgtac cgagatcgcg taatggctgg aaaaactctg gcgcaaggat
    22501 cagggccaga aaacccgcag ccagcgtcac gccagtatcg tagtgaccaa aatccagctc
    22561 gccgagatag gaaaaaccaa agtagaccgc caccagagca attgacagcg aggtaaaaaa
    22621 ttcgagaatg ccggaggata aaaacgccag ccgtagcact tccattgtcc gttggcggaa
    22681 atcttccgaa gcagaacgaa tactttcaat ttcagcttca ccacgaccaa aaatacgcaa
    22741 tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt aagcgagcaa gagcgagaaa
    22801 gttacgtcgg ttagcatcgg cagcccccat cccaaccagc gccataaaca acggaattaa
    22861 cggtgcagtg cccagcagaa tgagcgccgc agcccagtta gaggggaaga tcgccaccac
    22921 aatcagcaac ggcaccgaca ctgccagcgc catttgcggc agatagcgtg catagtaatc
    22981 atgcatatcg tcaatttgct cgagtaccag cgtcgcccag ctccccgcag gtttaccctg
    23041 aatccacgct ggccctgctt gttgcagacg gtcgagaacc tgacggcgga tggcaaagcg
    23101 gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac cagaccaccc atgcgcgcag
    23161 tacaaaggtc agaaccagta acgtaaaggg aagcagcagg gcttcacggg gaatattctc
    23221 cataatcata tgttgcagaa ttcgcgccat gaaccaggcc tgggcaatga tcaatatgcc
    23281 gctcacaaag cccagcagac gagaaatatt cagccaacgt tgggagatga cgctttgctg
    23341 ttttaaccag cgggttaact ctttttgacg agatttattc attgcacgct tagcaggtga
    23401 gttatcagaa ttatttgcag agcaatgtta caacggggaa aaaataaagg cgacccatag
    23461 tcgcatggtg tcgccttctt tacttttgtt actgatttgt aaaattattt tgcgtcagct
    23521 aaaccatcga ggtagcgttc cgcatcaagt gctgccatgc agcctgtacc ggccgaagta
    23581 atagcctggc gataaatgtg atccatcacg tcgcctgcgg caaagacgcc aggaatgctg
    23641 gtctgggtgg cattaccatg aatacccgac tgtactttga tgtagccgtt ttccagttcc
    23701 agctgccctt cgaaaatcgc agtattcggg ctgtgaccga tagcaacaaa cagaccggca
    23761 acgtcgagtg actcgatgtt atcgctgttt tgcgtatcgc gcagacgaac gccagtgaca
    23821 cccatttgat cgccggtcac ttcttccagc gtacggttgg tgtgcagaat gatgttgccg
    23881 ttctccactt tatccatcag acgcttaata aggatttttt ccgcgcggaa accgtcacgg
    23941 cggtgaatca gatgcacttc cgaagcgatg ttagacagat acagcgcctc ttcaaccgcg
    24001 gtattgccgc cgccgatgac cgcaactttc tggttgcgat agaagaaacc gtcacaggtt
    24061 gcacaagcag aaaccccacg gcctttaaag gcttcttcag agggcaggcc gagatagcgt
    24121 gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt attcgccgct atcgccagtc
    24181 agacggaacg gacgattctg caaatcaact ttgttgatgt gatcaaagat gatttcagtt
    24241 tcaaatttgg tggcatgttc gtgcatgcgc tccattaata acggaccggt cagatcgttt
    24301 ggatcgccag gccagttttc cacttccgtg gtggtggtca gttggccgcc tttttccatg
    24361 ccggtaatca gcacaggttg caggttggcg cgcgccgcgt agacagcagc ggtgtatccc
    24421 gccgggcctg aacccaggat aagcagttta ctgtgtttgg tcgtgcccat gagatcccca
    24481 tagttgttgg cagacaatgg gcaggattgt agggaattta cagacgtaaa aaaagagtat
    24541 gacgattttg ttaacaattt gtgcaatcgg cagcatcgat aagcaggtca aattctcccg
    24601 tcattatcac ctctgctact taaatttccc gctttataag ccgattaaat gatgaataaa
    24661 cgcccctgtt aatgaatatc tggcatgttg tactaaaaat cgatgttttg ctttgacaat
    24721 cccctggtgt tttgcgaaaa cattcgagga agaaaaaaaa cagtattctt atatgcgcat
    24781 aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa tctacgtatg gcgtggacag
    24841 acgccattcg tgatgtcgat agctgccaca aggcaacggt cttctcaccg tagacccagg
    24901 cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg acgggtagcg actctgaaca
    24961 gtgatgtttc agggtcagac aggagtaggg aaggaataca gagagacaat aataatggta
    25021 gatagcaaga agcgccctgg caaagatctc gaccgtatcg atcgtaacat tcttaatgag
    25081 ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta aacgtgtggg actttcccca
    25141 acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag ggtttattca gggctatacg
    25201 gcgctgctga acccccatta tctggatgca tcacttctgg tattcgttga gattactctg
    25261 aatcgtggcg caccggatgt gtttgaacaa ttcaataccg ctgtacaaaa acttgaagaa
    25321 attcaggagt gtcatttagt atctggtgat ttcgactacc tgttgaaaac acgcgtgccg
    25381 gatatgtcag cttaccgtaa gttgctgggg gaaaccctgc tgcgtctgcc tggcgtcaat
    25441 gacacacgga catacgttgt tatggaagaa gtcaagcaga gtaatcgtct ggttattaag
    25501 acgcgctaac acggaacagg tgcaaaatcg gcgtattttg attacactcc tgttaatcca
    25561 tacagcaaca gtactggggt aacctggtac tgttgtccgt tttagcatcg ggcaggaaaa
    25621 gcctgtaacc tggagagcct ttcttgagcc aggaatacac tgaagacaaa gaagtcacat
    25681 tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt gctgatcctt attgtcctgt
    25741 ttgccgtctg gttgatggct gccttactaa gctttaaccc ttcggacccc agctggtcgc
    25801 aaacggcctg gcatgaacct atccataatt taggtgggat gcccggtgcg tggttggcag
    25861 atacgctgtt ctttattttt ggcgtgatgg cttacaccat tcccgtcatt attgtcggcg
    25921 gttgttggtt tgcctggcgt catcagtcca gcgacgaata cattgattat tttgccgttt
    25981 cgctacgcat cattggcgtt ttggcgctca tccttacctc ctgtggtctg gcggcaatca
    26041 acgctgacga tatctggtat tttgcctccg gtggcgtcat tggcagctta ctaagtacta
    26101 cgctacaacc actgctacac agtagcgggg gaactattgc gctgctctgc gtttgggctg
    26161 cgggcctgac gctgttcacc ggttggtcat gggtgaccat tgctgaaaaa ctcggcggct
    26221 ggattttaaa cattctcacc ttcgccagta atcgtacccg tcgcgatgat acctgggtcg
    26281 atgaagatga atatgaagat gacgaagagt atgaagatga aaaccacggc aaacagcatg
    26341 aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg tcgtaaacgg ttggcggaaa
    26401 aattcattaa tccgatgggg cggcaaacag atgctgcgct gttctccggt aagcggatgg
    26461 atgatgaaga agatattacc tacactgcac gcggtgtggc tgccgaccct gacgacgtcc
    26521 tattttcggg caatcgtgca acgcagccag aatatgacga atacgatcca ttattaaatg
    26581 gtgcgccaat taccgaacct gtcgctgtag cagctgctgc taccacggcg acacaaagct
    26641 gggctgcgcc ggttgaacct gtgactcaga cgccgcctgt tgcctctgtt gatgttccac
    26701 cttcgcaacc tacagtagcc tggcagcctg aaccgggtcc acaaacggga gagccggtta
    26761 ttgctcctgc accggaaggt tacccacagc agtcacaata tgcgcagccc gcagtgcaat
    26821 ataatgagcc gctgcaacaa ccagtacagc cgcagcagcc gtattatgca cctgcagctg
    26881 aacaacctgc gcaacaacct tactacgctc ctgcggcgga acaacctgtt cagcaaccgt
    26941 attacgcaac tgcgccggaa cagcctgcac aacagccgta ttatgcccct gcgccagaac
    27001 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    27061 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    27121 agccgcaacc tgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    27181 aacccgcgcg tccgccgctt tactactttg aagaagtgga agagaagcga gcccgtgaac
    27241 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    27301 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    27361 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    27421 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    27481 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    27541 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    27601 cccagcgcaa tcagtacgat tctggcgatc agtacaacga tgatgaaatc gatgcgatgc
    27661 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    27721 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    27781 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    27841 atccgttcac gctggatgat tttgaatttt caccaatgaa agcgttgctg gatgatggtc
    27901 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    27961 cgccacagca gcaatatcag cagccgcaac agccggttgc gccgcagccg cagtatcagc
    28021 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccagttgcgc
    28081 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
    28141 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
    28201 atggcgacag ccgtccgttg cataaaccga cgacgccgct gccttctctg gatttgctga
    28261 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggctcgcc
    28321 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgtcaat tactctccgg
    28381 ggccggttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    28441 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    28501 ttattcctgg caaaccctat gtaggtctgg agttaccgaa taaaaaacga caaaccgttt
    28561 atctgcgcga agtattggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    28621 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    28681 tgttggttgc ggggactacc ggttccggta agtctgtcgg tgtgaacgcg atgatcctga
    28741 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    28801 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    28861 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    28921 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    28981 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    29041 atccggtgct gaaaaaagaa ccatacattg tggtgttggt ggacgaattt gccgacctga
    29101 tgatgacagt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    29161 ccgcgggtat ccacctagta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    29221 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    29281 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    29341 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    29401 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    29461 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    29521 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    29581 tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    29641 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    29701 cgttcgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    29761 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    29821 tagagggaac tcccggtcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgcc
    29881 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    29941 gatctgaaaa gtcgcctgga taaagtcagc agcttccacg ccagctttac acaaaaagtg
    30001 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    30061 aacttattca actggcatat gactcaacct gatgaaagca ttctggtttc tgacggtaaa
    30121 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    30181 accggtaata caccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    30241 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    30301 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    30361 caggacgatc agcgcagcag ttatcaactg aaatcccagc aaaatggggc tgtggatgca
    30421 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    30481 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    30541 gcgtatgcgg ccagaaaatt tagcacagta tatcggccag caacatttgc tggctgcggg
    30601 gaagccgctg ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc
    30661 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    30721 ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc
    30781 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    30841 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    30901 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt ctcgtgcccg
    30961 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    31021 ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg aaacacgacg
    31081 tgccattgca gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    31141 ggcggatatg gccgaagtcg atgatagcgg taagcgggtc ctgaagcctg aattactgac
    31201 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    31261 gatttctgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    31321 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    31381 tgcgtctgaa gacgtcggta atgccgatcc acgagcgatg caggtggcaa ttgcggcctg
    31441 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    31501 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    31561 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    31621 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    31681 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    31741 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    31801 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    31861 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    31921 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    31981 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    32041 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    32101 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    32161 agagctggat gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc
    32221 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    32281 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    32341 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    32401 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    32461 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    32521 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    32581 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    32641 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    32701 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    32761 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccggaaga
    32821 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    32881 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    32941 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgcgagatct cttcctgctc
    33001 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgtcgcagca agtcggacaa
    33061 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    33121 tgcggttatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    33181 tccgtatatg aacggactgg aatatattgg ctaataccta atttttctga atctaaaaag
    33241 cgcctgcggg cgcttttttt tgtctccctt tgataccgaa caataattac tcctcactta
    33301 cacgtaatac tactttcgag tgaaaatcta cctatctctt tgattttcaa attattcgat
    33361 gtatacaagc ctatatagcg atctgctata gaaataatta cacaatacgg tttgttactg
    33421 gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta
    33481 ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca
    33541 atggccagca gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc
    33601 attccaacaa aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt
    33661 cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat
    33721 accggcgatg acaattacga cggcctgcac caggttcgcg cctgtttgcg tgggcgttcc
    33781 atgcgtcgcc gtgtctataa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg
    33841 cgcggtgaag gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc
    33901 aatatgcagc gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc
    33961 ggtacgctgg gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgg
    34021 ctgatgaact gctgcggcgg ctatctgaac cattacggcg actactcctc cgcgcaaatt
    34081 gcggaaggtc tgaactatac ctacggcggc tgggcagatg gcaacagccc gtcggatatc
    34141 gaaaacagta agctggtagt gctgtttggt aataaccctg gcgaaacgcg aatgagtggc
    34201 ggtggggtga cttactatct tgaacaggca cgccagaaat ctaatgcccg catgatcatc
    34261 atcgatccgc gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt
    34321 ccgggaacag atgccgcact ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg
    34381 ttggatcagg cattcctcga taaatattgc gttggctacg atgagaaaac cctgccagcc
    34441 agtgcgccga aaaatggcca ctataaagct tatattctgg gtgaagggcc agatggcgtg
    34501 gctaaaacac cggaatgggc ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa
    34561 ctggctcgtg aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag
    34621 cgtcacgcta acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt
    34681 aacgttggta ttaatggagg gaacagcggc gcgcgtgaag gttcatacag cttaccgttt
    34741 gtccgtatgc cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc
    34801 gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat
    34861 aagctggatg tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag
    34921 cattctgaaa tcaaccgtac ccatgaaatc ctccaggatg ataagaagtg cgagctgatt
    34981 gtggttatcg actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac
    35041 tgcaccgctt ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac
    35101 gtgattttca acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg
    35161 accagcgaac tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacccag
    35221 gaagagtgga tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg
    35281 tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt
    35341 gcttataaag ccttccgtga agatccgcag gcaaatccac tgaccacgcc atcgggtaaa
    35401 attgagattt attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc
    35461 gatgtgatcg atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg
    35521 aacaaacagt atccgctgca gcttacaggt ttccactata aatctcgcgt tcactcaact
    35581 tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt
    35641 gatgcccaaa aacgcggtat ccacaacggc gataaagtca ggatctttaa cgatcgtggt
    35701 gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc cgggtgtggt cgcactgggt
    35761 gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata agggtggttg tattaacgta
    35821 ctgaccactc aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt
    35881 caggttgaaa aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc
    35941 cagccgttgc accggttgca aaacctgcga gctggcttgt aaagactaca aagatttgac
    36001 accagaagtc agcttccgcc gcatttatga atatgctggt ggcgactggc aggaagataa
    36061 cggtgtctgg caccagaacg tgtttgccta ctatctgtcg atttcgtgca accactgcga
    36121 agatccggct tgtactaaag tctgcccgag cggtgcgatg cataaacgtg aagatggttt
    36181 tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac tgccatatgg cttgcccgta
    36241 tggcgcaccg caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga
    36301 ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct tgtccgctgc gggcgctgga
    36361 tttcggccct atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt
    36421 gccgcgagct cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc
    36481 gaccggggat accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat
    36541 ggcatgaatg gccgctgatg atcttcacgg tcttcgggca atgtgtagcg ggtggtttta
    36601 tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta
    36661 tcgcctgcat gtttggctta tgggtgctga tgggcattgg ctttatcgcc tctatgctcc
    36721 atcttggttc accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca
    36781 gtaacgaaat cgccagcggt tccatctttt ttgccgtagg cggcatcggc tggttgctgg
    36841 caatgctgaa aaagctgtca ccggcattgc gtacgctgtg gctggtagtg acgatggttc
    36901 ttggcgtagt ctttgtctgg atgatggtgc gtgtctataa cagcattgat accgttccga
    36961 cctggtacag catctggacg ccgatgggct tcttcctgac aatgtttatg ggcggcccgt
    37021 tactgggtta cctgttgctg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc
    37081 cagccatttc tgtacttgca ctggtagtga gtggcgtgat gtcagtgatg cagggcgcag
    37141 agctggcgac tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg
    37201 cgctgatgtc ctggcggatc gtgcttttgg ctgttgccct gtgcttgtgg attgcaccac
    37261 agctaaaagg ttatcagcct gcggttccgc tactatctgt ctcattcatt ctgcttctgg
    37321 caggggaatt aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg
    37381 tcgcaagcta atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg
    37441 ttaacgtgtc ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg
    37501 tcgccagtcc ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc
    37561 atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt
    37621 cattaaaggt ccctgacgcg tcagtcacaa cgaagacatc aaacccttct tcaatggctg
    37681 aaagcgccgg gaatgccaag caaacttcgg ttaccacacc ggcaataatt aactgttttt
    37741 tacctgtcgc tttgactgct tttacaaaat cttcgttatc ccaggcgtta atattgcccg
    37801 ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag ttctggaact aatgggccgt
    37861 taggaccggt ttcaaaacta gtggtaagaa tagttggtaa gttgaaatac ttagccaggt
    37921 cacccagcgc cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa
    37981 gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat
    38041 acggtttggt catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac
    38101 gcgatgcatt gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt
    38161 ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt
    38221 aatcatctct tcagtattag cgttatgaat gagtaatata aatcgataat accgccgttg
    38281 cgatatgcgc gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa
    38341 atttgaactt cctcacggtt ttaattcatg tccacgtata cccggcctgt catgcttttg
    38401 ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt
    38461 tggctcgccc aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt
    38521 accggcaacc ttgtcggtac attgctgaca gggtatgtca ttaaacgcat tggctttaac
    38581 cgcagctatt atctggcctc cttcattttt gccgctggct gtgccggcct tggcctgatg
    38641 attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt
    38701 tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg
    38761 cttgctgcgt atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc
    38821 aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta caggtttgac gttggcaggg
    38881 atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg
    38941 tcaattacgt caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc
    39001 tcaggaatcg ttctgggatc tctatatggc ctgatgccgt tgtacctcaa tcacaaaggg
    39061 gtgagcaatg ccagcattgg tttctggatg gcggtactgg tcagtgcggg tatcctcgga
    39121 caatggccga tcggacgtct ggcggataag tttggtcgac tgctggtgtt gcgtgttcag
    39181 gtatttgtcg tcattctcgg cagtatcgcg atgcttagcc aggcggcgat ggccccagcg
    39241 ttattcatcc tcggtgccgc tggctttacg ctatatccgg tggcgatggc ctgggcttgc
    39301 gagaaagttg aacatcatca actggtggcg atgaaccagg ccttactgtt gagctatacc
    39361 gtgggaagtc tgcttggccc gtcatttacc gctatgctaa tgcagaattt ctccgataat
    39421 ttattgttta tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gcttcgcaac
    39481 gccggtcata cgccgaaacc cgttgctcac gtgtaaatga attcaagcag agtgtgaact
    39541 tactgtttca cactctgctt ttttgtttct tctatctgac ttgctttatt ccaaatttta
    39601 ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc atttcgctat ttccgcactt
    39661 cttatttgcc gcgcataatc cctcgtttta cagatgcccc tttaattttg gcgaaggatt
    39721 tgtctatggc tgggaatgtt caggaaaaac agctgcgatg gtacaacatt gcgctgatgt
    39781 cttttatcac tgtctggggt tttggcaacg ttgttaataa ctatgccaac caggggctgg
    39841 tggttgtttt ttcatgggtg tttatctttg cgctctattt cacaccttat gcgctaattg
    39901 ttggacagtt aggctcgacc ttcaaagatg ggaagggcgg ggtcagtacc tggattaaac
    39961 acacgatggg acccggactg gcttacctcg ccgcgtggac ctactgggtg gtgcatattc
    40021 cctatctggc acaaaaaccc caggcaatac tgattgcgct cggttgggcg atgaaaggcg
    40081 acggttcgct aatcaaagaa tattcagtcg tagcgttaca ggggttaacg ctggtgctgt
    40141 ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc gctgaaaatc gtcggttctg
    40201 tggcagggat tgcgatgttt gttatgtcgc tcctgtatgt ggcgatggcg gtaaccgcgc
    40261 ctgcgattac tgaagtgcat attgcgacca caaacattac ctgggaaacg ttcattcctc
    40321 atatcgactt tacctacatt accactattt caatgctggt tttcgcggtt ggcggagcag
    40381 agaagatttc tccttacgtt aatcaaacgc gcaacccagg aaaagaattt ccaaaaggga
    40441 tgttatgcct ggcggtgatg gttgcggttt gtgccattct gggctcactg gcgatgggga
    40501 tgatgtttga ctcgcgtaat atcccggatg acttaatgac taacggtcag tattacgcct
    40561 ttcagaagct gggcgagtat tacaacatgg gtaatacttt aatggtgatt tacgccattg
    40621 cgaataccct gggacaggta gctgcgctgg tattctcgat tgatgccccg cttaaagtgc
    40681 tattaggcga tgctgatagc aaatatattc cagccagttt atgtcgtacc aacgcttctg
    40741 gtacgcccgt taatggctat tttctgaccc tggtgctggt ggcgatcctg attatgctgc
    40801 cgactctcgg catcggtgat atgaacaatc tctataaatg gctgttgaat cttaactcgg
    40861 tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt tattgcagtc gttcgcctgg
    40921 cgcagaaata taaaccagaa tatgtcttta ttcgtaataa gcctctggca atgaccgtcg
    40981 ggatttggtg ttttgccttt accgcctttg cctgtttgac ggggatcttc ccgaaaatgg
    41041 aagccttcac tgcagagtgg accttccagt tggcgctgaa tgttgcaacg ccgtttgtgc
    41101 tggtaggatt aggactgata ttcccgctgc tggcgcgtaa agcgaatagt aaataattat
    41161 tgtggtcggt gacgctgact ctggcagatc tgacccgttt ttacataatc gagaaatgcg
    41221 cgcaagccgc aggacatata ctggcggttt gggtaataca tttggaagca gtgctgtaag
    41281 ggcgttagaa atagagctct gaattattta tgcagcgcgg aataaaaaag cggcaactca
    41341 ataaagttgc cgctttacgg ggaaattaga acattacctt atgaccgtac tgctcaagaa
    41401 tgcctttcac gcgttccatg gtctctttct tcggtggttt aacaccatca agtttgtatt
    41461 cttcacccat cgccacccat ttgtgtttgc ccagttcgtg gtaggggaga agttcgattt
    41521 tctcaacgtt acccatatca cgggtaaatt caccgaggcg atgcgccgaa tcgtcatcgt
    41581 cagaccagcc tgggacaaca acgtagcgga tccacacctt cacatttttg ttcgccagat
    41641 atttagcgaa ctccagcgtg cggtggttgg aaactccaac cagattttgg tggatctcgt
    41701 cgttcatctg tttgagatcg agcattacca ggtcggttac ttccagcagt tcatcaatca
    41761 ccggatcgta acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg ccttcttttt
    41821 tgcaggcgcg gaaccagtca cgaacaaact cagcttgcag gattgcttca ccgccggatg
    41881 cggtaacgcc gccgccggaa gcgttcataa agtggcgata ggtcaccact tccttcatca
    41941 aatcttcaac ggtaacttct ttaccgccat gcgtgtccca ggtgtcgcgg ttatgacaat
    42001 acaggcagcg catcaggcag ccctggaaaa aggtgataaa gcgaatacct gggccgtcta
    42061 cggttccaca ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc
    42121 agatgtggcc catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt
    42181 tggcagtcac cttaaagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc
    42241 cactttcgtg gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta
    42301 catagattga gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt
    42361 gaaacgtact gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc
    42421 catcgcgtca agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc
    42481 gatggatgct tcgtggtgga agtaaccatc catcagacct gccaggttgg tcttacgaac
    42541 ttcgtcgtct ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt
    42601 agcgtaagca aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc
    42661 acgaccgtgc atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg
    42721 ggtgttacca gttttcttac catacacaac gttagacgtg atggtcagaa cagactgagt
    42781 cgggatagcg tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag
    42841 gtcaacagcc aggtcatcta cacgcggatc gttgttacca aactgcgggt attcgccttc
    42901 gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc ggtttaactt tcgcatattt
    42961 gattgcagac agggagtcag cagcaacgga cagaccagcg ataccacacg ccatggtgcg
    43021 gataacgtca cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat
    43081 gtagtgaatg atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc
    43141 catgcgctcc atcacttcat cgtagttcag gagatcgcct ttgatcggtt cagacttcgg
    43201 accaacctgc attttcagtt tttcgtcaac gccgccgttg attgcgtaca gcatggtttt
    43261 cgccaggttt gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac
    43321 gcagcaagcg atagcgtagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc
    43381 atactgcaga gaagatgtgt cgatggacac tttagcggcg aatttcttga agttcagcgg
    43441 cagtttttca gaccacagaa tggtcatgtt cggttccgga gacggaccca tggtgtacag
    43501 ggtgttcagg aaacggaagc tgtttttggt aaccagggta cgaccgtcga ggcccatacc
    43561 accgatagat tcggttgccc agatcgggtc gccagagaac agttcatcgt attccggagt
    43621 acgcaggaag cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc
    43681 ttgttcggtg atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga
    43741 ggtacgaccg aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa
    43801 gtaagtccac tggatagctt cctgagcgtt ggttgccgga ccagagatgt cgtagccgta
    43861 tttcgcagcc atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg
    43921 cagacggata gtctgttcca ggtttacgcc gttttccaga tcagcctgca gagaggtgaa
    43981 ctgagcgtat ttgtctttca tcaggtagtc gataccgtac agcgcaacgc gacggtagtc
    44041 accgatgata cggccacggc cataagcatc tggcagaccg gtcagaacac cggatttacg
    44101 gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta
    44161 ttcagtgaag atttttttga tcatcgggtc cagttcgcgg ttgtacgctt tgcaggaacc
    44221 ttcgatcatt ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg
    44281 cagaccaaca actttttcca acgctttgtt gatgtagcca gcgtcgtgag aggtgatggt
    44341 ggaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca gtttaacgcc
    44401 ttccattact ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc
    44461 gtcaccctcg tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg
    44521 ccagtcacct ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat
    44581 gtaacaccta ccttcttaag tggatttttt atttactgcg tacttcgaca accattaatg
    44641 gtgatcgttt tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccgcc
    44701 gataatatta ccgatcgtaa ccggaatcag gttatcagtg atgaagttca tcacggtcag
    44761 gtgagaaaaa ttttccggtg cagaaccgac agcggtccag aattccggag atgcgaagtc
    44821 gcggattaca atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct
    44881 ggcaacaaac atcgcgaccg gcagcaccat gataaacgct ttgtccatca ggctgcggcc
    44941 agaataactc atccatactg ccagacatac catcaggttt gccaggatac caaggcagac
    45001 ggcctcaata aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca
    45061 ttgaccattt gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc
    45121 gccgaccagg ttgccaaaat agacatttag ccagttttta gccaactgac cccaggtgat
    45181 gcgcccactc gccttagcaa caacaatcaa cactgtggaa gtaaagagat cggctccgca
    45241 gacaacacaa agaatcagcc ccagagagaa gcaaatgccg ccaaccagtt ttgccatgcc
    45301 gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat
    45361 gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata
    45421 gacacccgcc tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt
    45481 gtcagctttc acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt
    45541 atagatgaga aattgatata gatcatatct cgcctggctt ataggcccgt aactcgcatg
    45601 gtttttatgc aaatacggag taaatatttg attatccaaa taaaaataaa ttttaaaaat
    45661 taacaaatga gttgaatttt ttccgcatcc tccgctaaaa cagttaatta aa
//
LOCUS       DAPGRN010000025        45214 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPGRN010000025 DAPGRN010000000
VERSION     DAPGRN010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.025"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1200)
                     /locus_tag="RHW33_001532"
     CDS             complement(<1..1200)
                     /locus_tag="RHW33_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1645053.1"
                     /translation="MMKTITKQPILFTDVPVADLRNSMKQDLNQNLIERLWNKIRDFF
                     LDSDKQKAFKSIHKYINTLSVLNYNSALNPDPNFNIDASSDLDSYLNLKFDNLSPKQK
                     QTTLCCFWNKIASSLPEPYNSTIKHNIIFYKVGENLMMRGTISIVNEVVKTYSLPIEK
                     DDNGYYDFSGLYLAHSNISGKDPNKDPDIDFGIDLGNCNCSNVNFEHTYFDSVKFTNT
                     NCTNANFNSCRFIKCDLTNMNCTGAILDNAVIYGKEKEPEMQYPEADQIIQRITYQKS
                     HGNETKGMILTNCSCVKTTFNWADLSESDCQYVDFSGANLSNTILPDIAMMKDTKLYR
                     TDLFNPILKTEAESTEEKDISPLAKIILDYIESDKNPESLNFEEKSTVIKIKQDIDNF
                     IFYNQHLK"
     gene            complement(1431..2405)
                     /gene="yajO"
                     /locus_tag="RHW33_001533"
     CDS             complement(1431..2405)
                     /gene="yajO"
                     /locus_tag="RHW33_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV1645054.1"
                     /translation="MQYNPLGKTNLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFALALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(2460..4322)
                     /gene="dxs"
                     /locus_tag="RHW33_001534"
     CDS             complement(2460..4322)
                     /gene="dxs"
                     /locus_tag="RHW33_001534"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV1645055.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRLMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(4347..5246)
                     /gene="ispA"
                     /locus_tag="RHW33_001535"
     CDS             complement(4347..5246)
                     /gene="ispA"
                     /locus_tag="RHW33_001535"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV1645056.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKK
                     LAEQSLDTSALEALADYIIQRNK"
     gene            complement(5246..5488)
                     /gene="xseB"
                     /locus_tag="RHW33_001536"
     CDS             complement(5246..5488)
                     /gene="xseB"
                     /locus_tag="RHW33_001536"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV1645057.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5694..7142
                     /gene="thiI"
                     /locus_tag="RHW33_001537"
     CDS             5694..7142
                     /gene="thiI"
                     /locus_tag="RHW33_001537"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV1645058.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(7196..7786)
                     /gene="yajL"
                     /locus_tag="RHW33_001538"
     CDS             complement(7196..7786)
                     /gene="yajL"
                     /locus_tag="RHW33_001538"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV1645059.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLMVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYHE"
     gene            complement(7749..8660)
                     /gene="panE"
                     /locus_tag="RHW33_001539"
     CDS             complement(7749..8660)
                     /gene="panE"
                     /locus_tag="RHW33_001539"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV1645060.1"
                     /translation="MNITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIFNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8828..9319
                     /gene="yajQ"
                     /locus_tag="RHW33_001540"
     CDS             8828..9319
                     /gene="yajQ"
                     /locus_tag="RHW33_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV1645061.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(9448..10812)
                     /locus_tag="RHW33_001541"
     CDS             complement(9448..10812)
                     /locus_tag="RHW33_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645062.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETAGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(10961..11851)
                     /gene="cyoE"
                     /locus_tag="RHW33_001542"
     CDS             complement(10961..11851)
                     /gene="cyoE"
                     /locus_tag="RHW33_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV1645063.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(11863..12192)
                     /locus_tag="RHW33_001543"
     CDS             complement(11863..12192)
                     /locus_tag="RHW33_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV1645064.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(12192..12806)
                     /locus_tag="RHW33_001544"
     CDS             complement(12192..12806)
                     /locus_tag="RHW33_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV1645065.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(12796..14787)
                     /gene="cyoB"
                     /locus_tag="RHW33_001545"
     CDS             complement(12796..14787)
                     /gene="cyoB"
                     /locus_tag="RHW33_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV1645066.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(14809..15756)
                     /gene="cyoA"
                     /locus_tag="RHW33_001546"
     CDS             complement(14809..15756)
                     /gene="cyoA"
                     /locus_tag="RHW33_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV1645067.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(16216..17691)
                     /gene="ampG"
                     /locus_tag="RHW33_001547"
     CDS             complement(16216..17691)
                     /gene="ampG"
                     /locus_tag="RHW33_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV1645068.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LFGGILMQRLSLFRALMIFGILQGASNAGYWLLSVTDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTAYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(17735..18313)
                     /locus_tag="RHW33_001548"
     CDS             complement(17735..18313)
                     /locus_tag="RHW33_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1645069.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            18618..18935
                     /gene="bolA"
                     /locus_tag="RHW33_001549"
     CDS             18618..18935
                     /gene="bolA"
                     /locus_tag="RHW33_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV1645070.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            19279..20577
                     /gene="tig"
                     /locus_tag="RHW33_001550"
     CDS             19279..20577
                     /gene="tig"
                     /locus_tag="RHW33_001550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV1645071.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            20823..21446
                     /gene="clpP"
                     /locus_tag="RHW33_001551"
     CDS             20823..21446
                     /gene="clpP"
                     /locus_tag="RHW33_001551"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV1645072.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            21572..22846
                     /gene="clpX"
                     /locus_tag="RHW33_001552"
     CDS             21572..22846
                     /gene="clpX"
                     /locus_tag="RHW33_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV1645073.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            23034..25388
                     /gene="lon"
                     /locus_tag="RHW33_001553"
     CDS             23034..25388
                     /gene="lon"
                     /locus_tag="RHW33_001553"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV1645074.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMHVVTAK"
     gene            25597..25869
                     /gene="hupB"
                     /locus_tag="RHW33_001554"
     CDS             25597..25869
                     /gene="hupB"
                     /locus_tag="RHW33_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV1645075.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            26061..27932
                     /gene="ppiD"
                     /locus_tag="RHW33_001555"
     CDS             26061..27932
                     /gene="ppiD"
                     /locus_tag="RHW33_001555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV1645076.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDVAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            28083..28454
                     /locus_tag="RHW33_001556"
     CDS             28083..28454
                     /locus_tag="RHW33_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV1645077.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            28560..28958
                     /gene="fadM"
                     /locus_tag="RHW33_001557"
     CDS             28560..28958
                     /gene="fadM"
                     /locus_tag="RHW33_001557"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV1645078.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(29010..29705)
                     /gene="queC"
                     /locus_tag="RHW33_001558"
     CDS             complement(29010..29705)
                     /gene="queC"
                     /locus_tag="RHW33_001558"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV1645079.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     AVMAAMKQKTGLK"
     gene            complement(29770..31470)
                     /locus_tag="RHW33_001559"
     CDS             complement(29770..31470)
                     /locus_tag="RHW33_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV1645080.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEKALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSADGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            31570..32388
                     /gene="cof"
                     /locus_tag="RHW33_001560"
     CDS             31570..32388
                     /gene="cof"
                     /locus_tag="RHW33_001560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV1645081.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            32541..32999
                     /gene="decR"
                     /locus_tag="RHW33_001561"
     CDS             32541..32999
                     /gene="decR"
                     /locus_tag="RHW33_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV1645082.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            33029..34801
                     /locus_tag="RHW33_001562"
     CDS             33029..34801
                     /locus_tag="RHW33_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV1645083.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            34794..36575
                     /locus_tag="RHW33_001563"
     CDS             34794..36575
                     /locus_tag="RHW33_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV1645084.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYEQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDNNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            36756..37094
                     /gene="glnK"
                     /locus_tag="RHW33_001564"
     CDS             36756..37094
                     /gene="glnK"
                     /locus_tag="RHW33_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV1645085.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            37124..38410
                     /gene="amtB"
                     /locus_tag="RHW33_001565"
     CDS             37124..38410
                     /gene="amtB"
                     /locus_tag="RHW33_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV1645086.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(38459..39319)
                     /gene="tesB"
                     /locus_tag="RHW33_001566"
     CDS             complement(38459..39319)
                     /gene="tesB"
                     /locus_tag="RHW33_001566"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV1645087.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            39537..40109
                     /locus_tag="RHW33_001567"
     CDS             39537..40109
                     /locus_tag="RHW33_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HDV1645088.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(40140..40451)
                     /locus_tag="RHW33_001568"
     CDS             complement(40140..40451)
                     /locus_tag="RHW33_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV1645089.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            40614..40710
                     /gene="ffs"
                     /locus_tag="RHW33_001569"
     ncRNA           40614..40710
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RHW33_001569"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            40830..41183
                     /locus_tag="RHW33_001570"
     CDS             40830..41183
                     /locus_tag="RHW33_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HDV1645090.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(41225..42775)
                     /gene="pdeB"
                     /locus_tag="RHW33_001571"
     CDS             complement(41225..42775)
                     /gene="pdeB"
                     /locus_tag="RHW33_001571"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV1645091.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(42939..43409)
                     /locus_tag="RHW33_001572"
     CDS             complement(42939..43409)
                     /locus_tag="RHW33_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV1645092.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(43525..44076)
                     /gene="maa"
                     /locus_tag="RHW33_001573"
     CDS             complement(43525..44076)
                     /gene="maa"
                     /locus_tag="RHW33_001573"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV1645093.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(44248..44466)
                     /gene="hha"
                     /locus_tag="RHW33_001574"
     CDS             complement(44248..44466)
                     /gene="hha"
                     /locus_tag="RHW33_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV1645094.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(44492..44866)
                     /gene="tomB"
                     /locus_tag="RHW33_001575"
     CDS             complement(44492..44866)
                     /gene="tomB"
                     /locus_tag="RHW33_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV1645095.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT        11387 a        11129 c        11883 g        10815 t
ORIGIN      
        1 ctttaaatgc tgattgtaga aaataaaatt atcgatatct tgttttattt taataactgt
       61 tgatttttct tcaaagttta atgactcagg gtttttatct gattcaatat agtcaagaat
      121 aatttttgct aatgggctaa tgtctttttc ttctgttgat tctgcttctg tttttaatat
      181 cggattaaaa aggtcggtac ggtaaagttt cgtgtctttc atcatggcga tgtcaggaag
      241 aatagtgttt gaaagattag ccccgctaaa gtccacatat tggcaatcac tttctgataa
      301 atctgcccaa ttgaaagtgg ttttaacaca ggagcagtta gttagaatca tccctttcgt
      361 ttcattccca tgtgatttct gataagttat cctttgtatt atttgatctg cttctggata
      421 ttgcatctca ggttcttttt ctttaccata tatcaccgca ttatcaagaa ttgcgccagt
      481 gcaattcata tttgttagat cgcatttgat aaatctacaa ctgttaaaat tcgcatttgt
      541 acaattcgta tttgtaaact tgacgctatc aaaataagta tgttcgaagt tgacatttga
      601 gcagttacag tttcccagat ctataccgaa atctatatcg ggatctttat tgggatcttt
      661 accggaaata ttgctgtgtg ctagatatag accgctgaaa tcgtaatatc cattgtcatc
      721 cttttctatt gggagtgaat aagtctttac tacctcgtta acaatagaga tagttccacg
      781 catcatcaga ttttctccga ctttataaaa aataatgtta tgtttgatgg tgctgttgta
      841 tggctctggc aatgaggatg caattttatt ccagaaacaa caaagcgtgg tctgcttttg
      901 tttgggcgag aggttatcaa atttcaggtt taaatatgag tctaaatctg acgatgcatc
      961 tatattaaaa ttgggatctg gatttaacgc tgaattataa tttagaactg acaatgtatt
     1021 gatgtattta tgaatcgatt taaatgcctt ttgtttatca ctgtccagaa aaaaatcacg
     1081 aattttgttc catagccttt caataagatt ttgatttaaa tcttgcttca ttgagtttct
     1141 taaatctgca accggtacat cagtaaacag gatcggttgt ttggtgatgg ttttcatcat
     1201 gattgtaatc ctcttacgtt gactaaataa gacagtttta ggggatatta cctggagaaa
     1261 ctatcaaaaa tcgctcaaag ggatgaagac gatgaatagc tgcaaactca ttcacgaaga
     1321 atttctcttg cagaggtgga ttatggttac agaaggtgat gggacgtacc ttgtccccat
     1381 catcgcagac gatggggaat tcagcagata aaatgggtga aaagcgaaat ttacttaaat
     1441 cctacgaccg gatgcggttt atacggcgtt tccagttcag caatctgttc cggcttcaac
     1501 gtgatatcca ccgcgttcag tagttcatca agttgttctt cccgcgatgt accaataatg
     1561 ggtgcagcaa tacctggttt gctcaacaac caggccagcg caacttgtgc tcgtgtcgcg
     1621 cccagttcct cactgacgcc cgttaagcgt tctgcaattt gcgcgtcatt ttcatcgctt
     1681 tctttgtaga gatttttccc cacctcatcc gacaccaggc gtgcagtggt ttctccccac
     1741 ggacgagtca gacggccccg cgccagcggg ctccacggaa tcaccgccac gccctcctga
     1801 taacacagtg gcagcatctc gcgctcttct tcgcgataaa tcagattgta gtgatcctgc
     1861 atactgacaa actgcgccca gccgtgctgt ttttgtagtt ccagcgccag agcaaactgc
     1921 gaggcgtgca ttgatgacgc gccgatataa cgcgctttcc cggcttttac gacgtcgttc
     1981 agagcttcca gcgtctcttc gatcggtgtg ttgtaatccc agcgatgaat ttgcaggata
     2041 tcgacataat ccatgccgag acggcgcagg ctgtcgtcga tagagcgcaa aatttgtgca
     2101 cgggataatc cttccggtaa atcaccaacg cgatggaaca ctttggtcgc aacaaccacg
     2161 tcttcacgac gggcgaaatc ccgcagtgcg cgaccgacga tctcttcgct gctgccgtca
     2221 gaataactgt tggcggtatc aaagaaattt atgccgcctt ccagcgcgcg tttaattatg
     2281 ggacggctgc tttcttccgg cagtgtccat gcgtgattac cacgatcagg ctcgccaaag
     2341 gtcatacagc cgaggcaaag tcgggaaacg cgaaggttgg tttttcctaa ggggttgtat
     2401 tgcatgctgc cactcctgcc atgaataatt tcttaagcat agcaggagtg gagtagggat
     2461 tatgccagcc aggccttgat tttggcttcc ataccggcgg catcgaggcc gagttcggcg
     2521 cgcatttctt cctgagttcc ttgcggaata aagaagtccg gcagaccaat gttcagcacg
     2581 ggtactggtt tacgatgggc catcagcact tcgttcactc cgctacctgc gccgcccata
     2641 atggcgtttt cttctacggt gaccagcgct tcatggctgg cggccatttc cagaattaac
     2701 gcttcatcaa gcggtttcac aaaacgcata tcgaccagcg tagcgttcag cgattcggcg
     2761 actttcgccg cttctggcat cagcgtacca aagttaagga tcgccagttt ctcgccacga
     2821 cgcttcacaa tgcctttgcc aattggtagt ttttccagcg gcgtcagttc cacgccaacc
     2881 gcgttgccgc gcgggtagcg caccgcggac gggccatcgt tatagtgata gccggtatag
     2941 agcatcaggc gacattcgtt ttcatcgctc ggagtcataa tgaccatttc cggtatgcag
     3001 cgcaggtaag agagatcaaa agcgccctga tgggtttgac cgtcagcacc aacaatgccc
     3061 gcacggtcga tggcgaacag gaccggaagc ttttgaatcg ccacgtcatg caacacctga
     3121 tcataggcgc gttgcaggaa agtggagtaa atcgcgacaa tgggtttgta cccaccaatc
     3181 gccagaccgg cggcaaaggt caccgcgtgt tgctcggcaa ttgccacatc gaagtagcga
     3241 tccgggaatt tacgtgaaaa ctcgaccatg ccggaacctt cacgcatcgc cggagtaatc
     3301 gccatcagct tgttatcttt cgctgcggtt tcgcacaacc agtcgccaaa gatttttgaa
     3361 tagctcggca aaccgccgct acttttcggc aaacaaccgc tggagggatc aaatttaggc
     3421 acggcgtgga aagtgattgg gtctttttct gccggttcat aaccacgacc ttttttggtc
     3481 atgatatgca ggaactgcgg gcctttcagg tcgcgcatgt tcttaagcgt ggtgataagc
     3541 cccagcacat cgtgaccgtc aaccgggccg atgtagttaa agcccagctc ttcaaacaac
     3601 gtgccaggta ctaccatgcc tttaatatgt tcttcggtac gtttgagcag ctctttaatg
     3661 ggtggcacgc cagagaaaac ttttttcccg ccttcgcgca gtgaagagta aagcttaccg
     3721 gaaagcagct gcgccagatg gttgttaagc gcgccgacat tttcggaaat cgacatttcg
     3781 ttgtcgttga ggaccaccag catatcagga cggatatcgc ccgcgtgatt catcgcttca
     3841 aacgccatgc ctgcggtaat cgcgccatcg ccaatcacac agacggtgcg gcgatttttg
     3901 ccttctttct cggcagcaac cgcaatacca attccggcac tgatggaggt tgatgaatgc
     3961 ccgacgctta atacgtcata ttcgctttcg ccgcgccacg ggaacgggtg cagaccacct
     4021 ttctgacgga tggtgccgat tttgtcgcgg cgtccggtca aaattttatg cggataagcc
     4081 tgatgcccca catcccaaat caattggtca aacggagtgt tatagacgta gtgcagcgcc
     4141 acggtcagtt cgaccgtgcc cagcccggag gcgaagtgcc cgctggaacg gctcacgctg
     4201 tcgagtaaat agcggcgcag ttcgtcgcag agtttcggta aactctcttt cggcaacagt
     4261 cgtaactcct gggtggagtc gaccagtgcc agggtcgggt atttggcaat atcaaaactc
     4321 atcaggggcc tattaatact tattgtttat ttattacgct ggatgatgta gtccgctagc
     4381 gcttccagtg ccgaggtatc gagtgactgt tcagccagtt ttttcagcga ctggcgggca
     4441 tcgtcgatca gatcccgggc tttcttccgg gcttgctcaa gacccagaag tgcagggtag
     4501 gtacttttac caagttgctg gtcggcaccc tggcgttttc ccaacgttgc agtatctcct
     4561 accacatcca ggatgtcatc ctgaacctgg aaggcaaggc cgatgctctc tgcgtacttg
     4621 tcgagtactg gcagagcacg acgcccttta tctccggcgc ttaatgcacc aaggcgaacg
     4681 gcggcgcgaa tcaatgcgcc agttttatga cgatgtatac gctcaagcgc gtccagaggt
     4741 acgtgtttgc cttccgcgtc taaatctaat gcctgaccac cgcacattcc ggcaataccg
     4801 ctggcgctcg ccagttcaga aatcatcgaa attctgtcgc gatccgacac ttccggcata
     4861 tcggcatcgc ttagaatcga gaacgccagc gtttgtaaag catcgccagc gagaatcgcg
     4921 ttcgcttcgc caaacttcac atggcaggtc ggcaagccgc gacgcagatc gtcatcatcc
     4981 atcgccggta aatcatcatg aattaatgag taagcgtgga tacactctac ggcggcagcg
     5041 ggtgcgtcca gcgtgtttgt gctaacgccg aacatatgac cggtggcata aaccaggaaa
     5101 ggtcgcaggc gcttaccacc taataatgcg ccatactgca tggtttcgac cacgggagtg
     5161 ttctgaaagg gcagtggggc gataaaacgg ctcagcgcct ggttggcctg cttaacgcag
     5221 gcttcgagtt gctgcggaaa gtccattact cattgtccgg tgtaaaaggg gttagagagg
     5281 cgtcttcatt gtcagacagc agaatttgta cgcgctgttc ggcttgttgt aatttggcct
     5341 gcccctgacg tgccagctgc acgccgcgtt cgaactcgtt cagcgcctct tccagcggca
     5401 ggtcgccact ttccagacgg gttacaatct gttccagctc gctcagcgcc ttttcaaagc
     5461 tggcgggcgc ctcatttttc ttcggcataa tgaatgtctg actctcaata tttttcgccc
     5521 cgtcatggta acggactcag ggcaaatagc aaataacgcg caatggtaag gtgatgtgcg
     5581 cagcaaagcg atgttagtgg tatacttccg cgcctggatg cagccgcagg tgtgggctgc
     5641 tgtatttttc cctatacaag tcgcttaagg cttgccaacg aaccattgcc gccatgaagt
     5701 ttatcattaa attgttcccg gaaatcacca tcaaaagcca atctgtgcgc ttgcgcttta
     5761 taaaaatcct taccgggaac attcgtaacg ttttaaagca ctatgatgag acgctcgctg
     5821 tcgtccgcca ctgggataac atcgaagttc gcgcgaaaga tgaaaaccag cgtctgacta
     5881 ttcgcgacgc cctgacccgt attcctggta tccaccatat tctcgaagtc gaagacgtgc
     5941 cgtttaccga catgcacgat attttcgaga aagcgttggt tcagtatcgc gatcagctgg
     6001 aaggcaaaac cttctgcgta cgcgtgaagc gccgtggcaa acatgatttt agctcgattg
     6061 atgtggaacg ttacgtcggc ggcggtttaa atcagcatat tgaatccgcg cgcgtgaagc
     6121 tgaccaatcc ggatgtgact gtccatctgg aagtggaaga cgatcgtctc ctgctgatta
     6181 aaggccgcta cgaaggtatt ggcggtttcc cgatcggcac ccaggaagat gtgctgtcgc
     6241 tcatttccgg tggtttcgac tccggtgttt ccagttatat gttgatgcgt cgcggctgcc
     6301 gcgtgcatta ctgcttcttt aacctcggcg gcgcagcgca tgaaattggc gtgcgtcagg
     6361 tggcacatta tctgtggaac cgctttggca gctcccaccg cgtgcgtttt gtcgctatta
     6421 atttcgagcc ggtcgtcggg gaaattctcg agaaaatcga cgacggtcag atgggcgtta
     6481 tcctcaaacg tatgatggtg cgtgccgcgt ctaaagtggc tgaacgttac ggcgtacagg
     6541 cgctggtcac cggcgaagcg ctcggccagg tgtccagtca gacgctgacc aacctgcgcc
     6601 tgattgataa cgtctctgat acgctgatcc tgcgtccgct gatctcttac gacaaagagc
     6661 acatcatcaa cctggctcgc cagattggca ccgaagactt tgctcgcacg atgccggaat
     6721 attgcggtgt tatctccaaa agcccgacgg tgaaagcggt taagtcgaag attgaagcgg
     6781 aagaagagaa gttcgacttc agtattctcg ataaagtggt tgaggaagcg aataacgttg
     6841 atatccgcga aatcgcccag cagaccgagc aggaagtggt ggaagtggaa accgtcaatg
     6901 gctttggccc gaacgacgtg atcctcgata tccgttctat cgatgaacaa gaagataagc
     6961 cactgaaagt tgaagggatt gatgtggttt ctctgccgtt ctataaactg agcaccaaat
     7021 ttggcgatct cgaccagaac agaacctggc tactgtggtg tgagcgcggg gtgatgagcc
     7081 gtctgcaggc gctctatctg cgcgagcagg gctttaacaa tgtgaaggta tatcgcccgt
     7141 aatttgtagt ttttacgtcg catctggtca gatgcgacgt ttgccgcatc cgacactact
     7201 cgtgataatt ataaatccct gccgccatca ccagttgtga tgccacttca tgggctttct
     7261 cgcgcccaac cagcaggtcg ataattttca gaccaaagtc gatagctgta cccggcccct
     7321 ggctggtcag caattttacc cgtgcatccc agacgacgcg cttgtcctgc cattgttcag
     7381 cgggaatttt gtctttcagc gtcgggaagc cggtcatatt gccaatcgga aagatatcgt
     7441 gcggcaccag cacggtggct ggcgcggcgc aaatagccgc gacgatacgc ccggaacggt
     7501 ggaactgttt aacggtttca accagcaggg tactatcgcg aaaacactct gcgcctttaa
     7561 tgccaccagg cagtacgatc acgtcatatt cgccatcagc cacttcgacc agcggcgcat
     7621 ccgccagcag cttcacgccg cgcgagcagg taattgccag gttgccatcg ctggctacgc
     7681 tggcagtggt gactttgata ccgccgcgaa ccatgaggtc gatcgtggtg acggcttcag
     7741 tctcttcact accaggggcg aggcaaacca gtgccgatgc gctcatattc actctccttt
     7801 ctttttacca tttcaaacag gcgggtgttt tccggtacgg caatcccatg cgcgcgggcg
     7861 cggcgtaaga gaaaaccatt gatatagtcg atttcggtgt ggcgcagcgc gcggatatcc
     7921 tgcaacatcg acgagatatt ttccgctgtg gcatcaatca cctgcatcac gtaatcacgc
     7981 aaatcttctg ctgaagtatg atgcccttcg cgttcgatca ccgccgcgac ttcttcgcat
     8041 atctgcataa tttcttgcgg atgatgacgt aattcaccgt tcgggcaatt gaagatggcg
     8101 gtcagtggat taatcacaca gttgactgcc agcttgcgcc acagctcggc gcgaatattg
     8161 ttatgccagg cgacgtcagg caacactgtc tgcaaaatat ccgccagata gctgtaatca
     8221 ccgtcctgtt gccgtgccgg gccaatatgc gtgataccgt ttgccacatg aataatgaca
     8281 ttgccgtcgc ggcgggcggc atgggtggtg gtgcccatca gtaatggctg ctgaatgttt
     8341 tgcaactctt cgatggtgcc catgccgttg tgaattaaca gtattggcgt ggttacaggc
     8401 agtgtggacg cgaggctttt gacggcatcg gaaacctgcc atgctttcag cgtcaccagg
     8461 agcagatcgc tggtggcaag aaaatcggga tcgttggcgg tcagcgattc gttaaatatc
     8521 gaaccatcag tctcaaccag attcacacta caataaggtt gcggtacgcg cagccagccc
     8581 tgaacttcgt gaccctgttt acaaagtgct gtaagccata attgccctaa ggcaccgcat
     8641 cccaatacgg taatattcat tgttcctcct cacccgcaac cactccgggt gttcaataag
     8701 gctatccctt aattgtgcat gctgttgcga ctatgcacaa ttaagggata cgtcctggtg
     8761 caggactgtt ggttatttaa ctttgcaggt attatgcttc gcatcaaaaa tgaagggaga
     8821 ggaaaagatg ccatctttcg atattgtctc tgaagttgat cttcaggaag cacgtaacgc
     8881 ggtcgataac gcgagccgcg aagtggagtc ccgttttgac ttccgtaacg ttgaagcctc
     8941 atttgagctg aacgacgcca gcaaaaccat caaagtgttg agcgagtccg acttccaggt
     9001 caatcagttg ctggatattt tgcgtgccaa gctgctgaag cgcggcattg aaggcagttc
     9061 actggatgta ccggaaaata tcgttcatag cggtaaaacc tggtttgtgg aagcgaaact
     9121 gaaacagggc attgagagcg ccactcagaa aaaaatcgtc aagatgatca aagacagcaa
     9181 actgaaagtg caggcgcaaa ttcagggcga tgagatccgt gtgacgggca aatctcgtga
     9241 tgatttgcag gctgtcatgg cgatggtgcg cggtggcgat ctgggtcagc cgttccagtt
     9301 caaaaacttc cgcgattaat cgcgacaacc tgcgctttgt tcatgtcaga tgcggcatga
     9361 acgcctggtt cggcctacaa aggtttgtaa ttcaggaact ttgtaggcct gataaacgta
     9421 gcacagcagg caatttcgcc tgttttttta cgcctggcga attgcctgct caacctcaaa
     9481 gcgattcgtc actttgctgt cgattttcac ataagctgaa tgttcttctt ctgcaatcaa
     9541 cacttctttg acacccgcag tttccagcaa acgcactttt aacgcctcgt ttgcggcaat
     9601 gtccgccggg atttcaatgc gtaagctgct gacatatggc ggttctttca tggtactggc
     9661 gactgccagc cacactgctg ccagcattgc gccagcgaga aataccccct gaccgtcaaa
     9721 catgccgtca atccagccgc ccagcgaacc gccaatcgcc acgccaagaa actggctggt
     9781 ggagtaaaca cccatcgccg tacctttgta acctgctggc gactctttac tgataagtga
     9841 gggcaggagg gcttccatca aattaaacgc cacaaagaaa agctgcacgc cgaccaccag
     9901 ttgccagaac tgcgtttgcg cgttccacaa cacaatttcc gcaaccacga tcaacccgac
     9961 gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa tgaaaggcac
    10021 gaccgagcca aaggcgatta gcattgtcgc cagatagacc ttccagtgtt cagccgccgg
    10081 gaaccccgca tcagccagtt gtccgggcag ggcaacaaac gtcgacatca gcaaaatatg
    10141 cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca ctttactgaa
    10201 actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt tgggcacaac
    10261 ccaaatggtc aacgcaatgc cggtcgttgc cagaatagcg atcatccaga acagcgcgtg
    10321 cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa aggtaatgcc
    10381 aaagctcacg ccgataaacg ccattgcttt ggtgcggttt tgttcgcgcg tgagatcgga
    10441 aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgcac ggcccagaat
    10501 aattccccag atggagtcag aaagcgcagc gataacgcta ccggcggcaa acaccgccag
    10561 cccaccgaca attaatggtt tgcgaccaat gcggtcggaa agcaggccaa acggaatctg
    10621 aaaaacggcc tgcgtcagac cataaatacc aatggcaata ccgattaatg cttcgctggc
    10681 accttgcaga gccatgccgt acgtggtcag aaccggcaga accatgaaca tgcccagcat
    10741 gcgcaacgag aatacggtcc ctaaacccca ggtcgcgcgc cgctcacctg gcgtcatttt
    10801 ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg gcaaacgaat
    10861 ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgtttc taatgctaag
    10921 aaaaaaggtg ccgtagcacc tttttaatag agaggttttg ttaccacaca gcagccagca
    10981 gcgtatgcga gtccggcacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg
    11041 cgatgataga gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac
    11101 cgcgcagggc catacctaac caccagacgc tcaccgccgc ggcgaccacc agatatttat
    11161 atccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca
    11221 gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg
    11281 ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca
    11341 ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt
    11401 agccaatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagactgagt
    11461 ggcgtttcat gtacaggcta taaacgccga cataaaccac aaagcccatc acccccagcc
    11521 agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg
    11581 tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg
    11641 tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg
    11701 ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca atgctgccct
    11761 ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt
    11821 tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt
    11881 gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagcga tgattagcac
    11941 ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa
    12001 gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac
    12061 ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga
    12121 caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt
    12181 agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc
    12241 acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgctggtc
    12301 aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg
    12361 tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccggg
    12421 cccatgccgt taacaatcag gtgatggaat tcatagattt ccatcccgat aaatccggcg
    12481 ccaaacaacc aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg
    12541 gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga
    12601 acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga
    12661 acggcatagg tagcaaacaa gatagagaac agaatgcagt cgctcatcag gtagatccaa
    12721 aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtgggcagtc
    12781 gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg
    12841 ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa
    12901 gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat
    12961 atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga tacctgcacc
    13021 gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt
    13081 ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata
    13141 gaacggaggc ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt
    13201 caggtcacgg ttctggtcgc ggtcacgaat agaaacgtac atctgaataa cgaggcagag
    13261 aatacctagc gcaatcagta ctgcaccgct ggctgcaatc atcagcatgg tgtggaactg
    13321 cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg cgtacagtgg
    13381 cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac cccaggtttc
    13441 gttcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga agcagccgaa
    13501 gaccacgccg ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgtag
    13561 aacgaagtcc gcgcccggta ctgccagcag cacgccagtc ataccgccca ccgagaaggt
    13621 gacgataaaa ccgatagtcc acagcatcgc agaatggaac acgatgcggc cctgatacat
    13681 ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat
    13741 accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac
    13801 gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag
    13861 acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag
    13921 gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc
    13981 acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac
    14041 ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca
    14101 ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac
    14161 gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca
    14221 gtaatcgaca ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca
    14281 gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac
    14341 ggtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccaatctg
    14401 cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga agaagatcat
    14461 aatcacgccg tgcgcggtaa agatctgatc gtagtggtga ggtggcagga agcccgcttc
    14521 gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag
    14581 caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt
    14641 cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc
    14701 cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac
    14761 tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga
    14821 ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcgctgtgct caccttctgg
    14881 ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaatcacat cagcaaacaa
    14941 gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt
    15001 ttcgaacgca gccatgtcag acatggtgtt cggcgactgt ttcgcttttg cgacccattg
    15061 gtcgaatgcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg
    15121 gccgctgtag ctggcggaga taccgtcata agtgccgggt tcgttggcga tcagatgcag
    15181 gcgagtctgc ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga
    15241 gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat
    15301 ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc agtccatgga
    15361 aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc
    15421 gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca
    15481 gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt
    15541 gctcgcacgg tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat
    15601 caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt
    15661 gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa
    15721 ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat
    15781 ctggtggcgt ttaaagtgtg tgggcatttt acgggaaggt tacattactg taaacatgat
    15841 taaattcctg ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaggtt
    15901 gattttaaga agctgttgca aaagggcgtt ataacaaaag gggagtgcag aaggagtctg
    15961 ggcgggcaat tggtataacc aatgcgaaat aaaacaatta tttaacaaat aattatcatt
    16021 ggagtgacaa ctgggtggtg agaaaataaa gactcattag ctgaatcgtg taagaaaaat
    16081 agaatttata aatggcgcca ataatttcca aacgctaagc cgcacaaaag aacaaatatt
    16141 aataagcgat gctgatgttt atttagccgt aataattacg gcgagtgatt actacagcta
    16201 aataatattt acagattacg tcagatgcgt ttttcgtagc gccagataat ccagcaaacc
    16261 accaagcacg acgccagaaa gggcgactaa aaccccgact tccagcagag caggcaggaa
    16321 agagaagtgc gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca cggccaacag
    16381 gctgacgccc gccgccagtg tccacatggc aaaggcataa cctgccggat atgcggtacg
    16441 ggagataaag ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa ccagcagcaa
    16501 aataagccct ggtacggcag cggcgacgga gaatagatag aatgtcgacc agccgtgagc
    16561 ttcaacaaac caacctgcta ctggtcctac ataaacccgt cctacagcag aaagtgccga
    16621 gagcagagca aactgggtgg cggaaaatga cttattacac aaagtcatta acagcgctac
    16681 aaacgccgat gtacccatcc caccacacag gttttcgaaa aagacggccg cgcccatgct
    16741 gtagagatgc ttatcagtca cggaaagcag ccagtaacct gcgttggacg cgccttgtaa
    16801 aatgccgaaa atcataagcg cacggaacaa tgacaggcgc tgcatcaaaa ttccgccgaa
    16861 caatgcgccg acgatggtcg ccagcagacc gagcgttttg ttaactacac ccacttcgcc
    16921 agcatcaaat ccgacgccgc gaatcaaaaa ggtggttgtc aggctcatgg cgaaagcatc
    16981 gcccagcttg tacagaacga ttaacagcag aatcagccac gcattattgc ggccaaagaa
    17041 atctcgcaga ggtgcaacaa ccgcttgttc cagcgttttt ggcacaggaa tggtgtcggt
    17101 tggttctggt gcaagcaacg tcgcgataat acaagggatc aacagcgccg ccatcaacca
    17161 gtacatgccc tgccagccca gccatttatc tgccagccac agggctaggc cgccggaaac
    17221 cagcatccct aaacggtaac ccagaacgct gattgccgcg cccgcaccac gttcttctgc
    17281 cgggagcaca tcggttttcc acgcatcgaa gacaatatcc tgggaggcag agcaaaaagc
    17341 gatcaccacc gccagcgccg ccatccagcg gagttgggtg cctggttcga gaaaacccat
    17401 cgccgcaatg gcgactaata acaggatttg cgtggcgagc agccaaccgc gccgccgccc
    17461 aaaaaatggg ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa aaacgtaagc
    17521 ctggcctacc agagagaaga aaccaatggt tttgagatcg atattctcga ccgtcatcca
    17581 ggcctgtaag gtgccggatg tcagggcgag cggtagcccg gaagcaaaac ccaggatcag
    17641 caatatggct gaacgcggct gttgaaaaat acgtaaatat tgactggaca tgggcgtcta
    17701 caggcccggc ttgcaccggg ccagagggca gaaattaacg cgcgttctgc ttgatgaatt
    17761 cgtgaatgct ggtgtcctga gacatatcag cgatggtatc ggtcagcaca ctgttaaccg
    17821 catcggcgat atttttgttg gaggcctgga acgcaccttc aacgttgtag ctggcacgat
    17881 agtttttggt cattttgttg ccattctgcg cggtagcgat gatggcgata tccgctttgg
    17941 tagcgatgtt gtagcgcacg ttgccctggg acacgtcagc atacagttgg ctaacgatga
    18001 tttgcagatt aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc atctgttttt
    18061 ccagcacttc ttgcagcagg aaacgcagat cgcgggaggc ggtcagggta acgatttgat
    18121 tatcgcgggt gacttttgcc agcgcctgat cggtacgctg atcggcacca ttaatgctta
    18181 cggtgacgcc catcaggctt ggatcctgct gtggcagtgt aatcgtcggg gaaacttcaa
    18241 tagttgttgg cggttttgcg catcctgcaa gcataaacag agcaactaac gggaagagga
    18301 tttttttgaa catgttcggg ctctcagaga ctcttaagcg tgtttggtaa aaattcccgc
    18361 catcataaca ttgccaacgg cgaggggaag taggtaaggc atgtaaattc atcatgttga
    18421 cgaaataatc gcccctggta aaagaaacgc tgatgcgagg cctgtgtttc aatctttaaa
    18481 tcagtaaact tcatacgctt gacggaaaaa ccaggacgaa acctaaatat ttgttgttaa
    18541 gctgcaatgg aaacggtaaa agcggctagt atttaaaggg atggatgaca tctcagcgtt
    18601 gtcggaggag atatttcatg atgatacgtg agcggataga agaaaaatta agggcggcgt
    18661 tccaacccgt attcctcgaa gtagtggatg aaagctatcg tcacaatgtc ccagccggct
    18721 ctgaaagcca ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa cgttttctga
    18781 atcgtcatcg aatgatttac agtactttag cggaggaact ctctactacc gttcatgcgc
    18841 tggctctgca tacttacact attaaggagt gggaagggtt gcaggacacc gtctttgcct
    18901 ctcctccctg tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac tgtcggcgct
    18961 tttccagtat gttgctaaag attttatgaa aaacggcctg cgggccgttt tgttttgtct
    19021 ggattttgcg ctttttgccc agcattcaga cgaaaattgc ccgggaattg tgaaaaaata
    19081 cgcgacagcg cgcaataacc gttctcgact cataaaagtg atgccgctat aatgccgcgt
    19141 cctatttgaa tgctttcggg atgattctgg taacagggaa tgtgattgat tataagaaca
    19201 tcccggttcc gcgaagccaa caacctgtgc ttgcggggta agagttgacc gagcactgtg
    19261 attttttgag gtaacaagat gcaagtttca gttgaaacca ctcaaggcct tggccgccgt
    19321 gtaacgatta ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga gctggtcaac
    19381 gttgcgaaaa aagtacgtat tgacggcttc cgcaaaggca aagtgccaat gaatatcgtt
    19441 gctcagcgtt atggcgcgtc tgtacgccag gacgttctgg gtgacctgat gagccgtaac
    19501 ttcattgacg ccatcattaa agaaaaaatc aatccggctg gcgcaccgac ttatgttccg
    19561 ggcgaataca agctgggtga agacttcacc tactctgtag agtttgaagt ttatccggaa
    19621 gttgaactgc agggtctgga agcgatcgaa gttgaaaaac cgatcgttga agtgaccgac
    19681 gctgacgttg acggcatgct ggatactctg cgtaaacagc aggcgacctg gaaagaaaaa
    19741 gacggcgctg ttgaagcaga agaccgcgta accatcgact tcaccggttc tgtagacggc
    19801 gaagagttcg aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca gggtcgtatg
    19861 atcccgggct ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt caccatcgac
    19921 gtgaccttcc cggaagaata ccacgcagaa aacctgaaag gtaaagcagc gaaattcgct
    19981 atcaacctga agaaagttga agagcgtgaa ctgccggaac tgactgcaga attcatcaaa
    20041 cgtttcggcg ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg taaaaacatg
    20101 gagcgcgagc tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat cgaaggtctg
    20161 gtaaaagcta acgacatcga cgtaccggct gcgctgattg acagcgaaat cgacgttctg
    20221 cgtcgccagg ctgcacagcg tttcggtggc aacgaaaaac aagctctgga actgccgcgc
    20281 gaactgttcg aagaacaggc taaacgccgc gtagttgttg gcctgctgct gggcgaagtt
    20341 atccgcacca acgagctgaa agctgacgaa gagcgcgtga aaggcctgat cgaagagatg
    20401 gcttctgcgt acgaagatcc gaaagaagtt atcgagttct acagcaaaaa caaagaactg
    20461 atggacaaca tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt actggcgaaa
    20521 gcgaaagtga ctgaaaaaga aaccactttc aacgagctga tgaaccagca ggcgtaattt
    20581 acgcagcata acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg tgggcttttt
    20641 tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta gcgtaacaac
    20701 aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg actagctgat
    20761 aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc caggagacgg
    20821 aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg gtgccgatgg
    20881 tcattgaaca gacctcacgt ggtgagcgct cttttgatat ctattctcgt ctacttaagg
    20941 aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg attgtggcgc
    21001 agatgctgtt cctggaagca gaaaacccag aaaaagatat ctatctgtac attaactccc
    21061 caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt atcaagcctg
    21121 atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg ctgaccgcag
    21181 gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac caaccgttgg
    21241 gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt ctgaaagtta
    21301 aagggcgtat gaatgaactt atggcgcttc atacgggtca atcattagaa cagattgaac
    21361 gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac ggtctggtcg
    21421 attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct atacttatcc
    21481 agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt cgtcgtgtgc
    21541 ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag atggctcagg
    21601 caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca agctgattgc
    21661 cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca tcattcgcga
    21721 agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc cgcatgaaat
    21781 tcgtaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag tgctggcggt
    21841 cgcggtatac aaccactaca aacgtctgcg caacggcgat accagcaatg gcgtcgagtt
    21901 gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc tgctggctga
    21961 aacgctggcg cgcctgctgg acgtcccgtt caccatggcc gacgcaacca cgctgaccga
    22021 agccggttat gtgggcgaag acgttgaaaa catcattcag aagctgttgc aaaagtgcga
    22081 ttacgacgta cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg acaagatttc
    22141 tcgtaagtca gacaacccgt ctattacccg tgacgtttcc ggtgaaggcg tacagcaggc
    22201 actgttgaaa ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg ggcgtaaaca
    22261 tccgcagcag gaattcttgc aggttgatac ctctaagatc ctgtttattt gtggcggtgc
    22321 gtttgccggt ctggataaag tgatttccca tcgtgtagaa accggctccg gcattggttt
    22381 tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc tggcgcaggt
    22441 tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc gtctgccggt
    22501 tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca aagagccgaa
    22561 aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg atctggaatt
    22621 ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa ccggtgcccg
    22681 tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc tgccgtccat
    22741 ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa gcaaaccgtt
    22801 gctgatttat ggcaagccgg aagcgcaaca ggcatctggt gaataattaa ccattcccat
    22861 acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc tctattctcg
    22921 gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag atggcgtgaa
    22981 gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc tctatgaatc
    23041 ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg gtggtttatc
    23101 cgcacatggt catcccctta tttgtcgggc gggaaaaatc tatccgttgt ctggaagcgg
    23161 cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca acggatgagc
    23221 cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag atgctgaaac
    23281 tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt atttctgcgc
    23341 tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg ccgaccattg
    23401 atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa ggctacatca
    23461 agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc gacgatccgg
    23521 cgcgtctggc ggataccatt gctgcacata tgccgctgaa actggctgac aaacagtccg
    23581 ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg atggaatcgg
    23641 aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag cagatggaga
    23701 aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa gaactcggtg
    23761 aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac gcggcgaaaa
    23821 tgccgaaaga ggcaaaagag aaagcggaag cagagctgca gaagctgaaa atgatgtctc
    23881 cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta caggtaccgt
    23941 ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc cttgataccg
    24001 accattatgg tctggagcgc gtgaaagatc gcatccttga gtacctcgcg gttcaaagcc
    24061 gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg gtaggtaaaa
    24121 cctccctggg ccagtccatc gccaaagcca ccgggcgtaa atatgtccgt atggcgctgg
    24181 gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc ggttctatgc
    24241 cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg ttcctgctcg
    24301 atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca ctgcttgaag
    24361 tgctcgatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg gattacgatc
    24421 tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca ccgctgctgg
    24481 atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg aacatcgcca
    24541 aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt gagctgaccg
    24601 tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg ggcgtgcgtg
    24661 gtctggagcg tgaaatctcc aagctgtgcc gtaaagcggt taagcagtta ctgctcgata
    24721 agtcattaaa acatatcgaa attaacggcg acaacctgca tgactacctt ggtgttcagc
    24781 gtttcgacta tggtcgcgct gataacgaaa accgtgtcgg tcaggtaacc ggtctggcgt
    24841 ggactgaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg gggaaaggca
    24901 aactgaccta taccggttcg ctcggcgaag tgatgcagga gtccattcag gcggcgttaa
    24961 cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac gaaaaacgcg
    25021 acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt gctggtattg
    25081 ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc gatgtggcaa
    25141 tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg aaagaaaaac
    25201 tactggcagc gcatcgcggc gggattaaaa cagtgttaat tccgttcgaa aataaacgcg
    25261 atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct gtgaagcgca
    25321 ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcat gttgtgactg
    25381 caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg ctaattcggg
    25441 cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc ccttattaag
    25501 gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac cttgaaggat
    25561 tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca attgatcgac
    25621 aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt agatgctatt
    25681 attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt aggttttggt
    25741 acctttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac cggtaaagag
    25801 atcaccatcg ctgccgctaa agtaccgagc ttccgtgcag gtaaagcact gaaagacgcg
    25861 gtaaactaag cgttgtcccc agtggggatg tgacgaagtt caagggcgca tctactgatg
    25921 tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa ttgcccccgt
    25981 ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca cagtcatcta
    26041 cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac agtctcgtgc
    26101 tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg agtggttacc
    26161 tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc agccgtgggc
    26221 aattcgagaa tgccttcaac agcgagcgta atcgcatgca gcaacagctg ggcgatcaat
    26281 actccgagct ggcagcgaac gaaggctata tgaaaaccct gcgtcaacag gtgctgaatc
    26341 gtctgatcga cgaggcgctg ctggatcagt acgcacgtga gctgaaactg ggtatcagcg
    26401 atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac ggcaaatttg
    26461 ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat cagtacgccc
    26521 aggcgctgcg taaccagctc actacccaac agctgattaa cggcgttgcc ggtaccgatt
    26581 ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgctcaacaa cgcgtggtac
    26641 gtgaagcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc gaacaggaaa
    26701 ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa ttccgcgtga
    26761 gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg gatatccaga
    26821 gctactacga ccagcatcag gatcaattca cccagccgca gcgtacccgc tacagcatca
    26881 tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat aaaggcggtg
    26941 attttgctgc gttagccaaa gaaaaatctg ccgatattat ctctgcccgt aacggcggcg
    27001 atatggggtg gttagaagat gccactatcc cggacgaact gaaaaatgct ggtctgaaag
    27061 aaaaaggcca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt gtacgtctgg
    27121 acgacattca gccagcgaaa gtgaaatcgt tagacgaagt gcgtgacgat gtcgcggcga
    27181 aagtgaaaca cgaaaaagcc ctcgatgcgt actacgcgct gcagcagaaa gtgagcgatg
    27241 cggcaagcaa cgacaccgag tctctggccg gtgcagagca agcagccggc gttaaagcca
    27301 ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc aagccggttg
    27361 ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc atcaactctg
    27421 acatcatcac cgtcgatggc gaccgcgcat tcgtgctgcg catcagcgag cacaaaccgg
    27481 aggcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt cagcacaaca
    27541 aagctgaaca acaggcgaaa gtggatgctg aaaaactgct ggttgatttg aaagccggca
    27601 aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa accttaagcc
    27661 gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca gcgaaagaca
    27721 aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg gcgctggatg
    27781 aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag ggtatcaccc
    27841 agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa gaggcgaaaa
    27901 tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca aaaaaatgct
    27961 ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga acattgccat
    28021 ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa ggagaaaaca
    28081 gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt gccggaatgt
    28141 ctcatagcgc gctggcggca gctcctgcgg cgaaaccgac ggcggtagaa accaaagcgg
    28201 aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac gaagaaggca
    28261 cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg aatggcgttg
    28321 gcctgaagaa agcgcaggcg attgtcagtt atcgtgaaga gtatggtccg tttaaaacgg
    28381 tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt aatctggcgg
    28441 tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa gaggtcatac
    28501 cagttatgac ctctgtactt ataacagttt ttataaaaac aacgtaaggt tattgcgcta
    28561 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
    28621 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
    28681 ttcactggat gacggcccat aacatcgctt tcgtggtggt gaatatcaat attaactatc
    28741 gtcgcccggc ggtattaagc gacctgttaa ctattaccag tcagttacag caattaaacg
    28801 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtagtagcgg
    28861 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
    28921 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
    28981 caagccgcat ccggcgacac ccggaataat tacttcaacc cggttttctg cttcatcgct
    29041 gccatcaccg ccggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
    29101 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
    29161 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
    29221 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
    29281 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcgcagacg
    29341 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
    29401 aaaatattac gccctgggac aaacgtattc gggatgccat cggcttcagg ttcataatca
    29461 ggcaccggaa tgctgtcacg cgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
    29521 tcaagaacct tatgcgcgcg tgcccccagt ttcagcgcca gttcgcgtgc cacgtcgatt
    29581 tctgcgcgat ggcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
    29641 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
    29701 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
    29761 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
    29821 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
    29881 cagcggcgtc agcgtcgcat ctgtcattaa ctggctaaaa accgctttca gggcattaaa
    29941 tcggttttcc tcatcaggca ttacttgcac cgcgtccagt gtcgattgca aatgggcata
    30001 tgctggagcg tcgaaaacat gtggccacag tggatcgcaa cgtagccatt gctccagagt
    30061 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
    30121 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
    30181 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
    30241 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccaatgcg gaatagtcca
    30301 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
    30361 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgtgg
    30421 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
    30481 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
    30541 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
    30601 acgtaaatgg taataatcat ggctttccag gcgcaccagt tcagctgtga attgtgttaa
    30661 gcgaaaagga cccgtgccaa tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
    30721 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
    30781 atgggtgacc tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
    30841 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
    30901 tagggttgaa cgaagataaa agtcccagcg taacccgtca gcagaggttt cccagtgatg
    30961 cgctaaatcg ccaatcgggc gctgggtatt attatcgaag cgggtcaggc cggaaaatat
    31021 ctgtccggcg agatgctgct cggcacggcc gggcaaaaag cccggttgta gcggttcgag
    31081 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
    31141 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
    31201 ctgctttccg gtttccagtg ccttttccat catcgcattg cgtagcgatt ctggcgtgac
    31261 cagaaagcgt agttgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
    31321 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
    31381 ggccagttcg ctgacggtga cggtttgcgg ctttccggcg gaaggttgcc acagacgttg
    31441 atactggtta agacggttga gcagtcgcat ataaacccgg aacaatatta tttaactatt
    31501 cactattact tccgtatata tcaggtgata ctcaatcacc attaaccgtg tcacagagtg
    31561 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
    31621 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
    31681 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
    31741 ctggatgcgt atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
    31801 ttacatcgtg atgatttacc tgcggacgtc gcggagctgg tgctgtatca gcaatgggat
    31861 acccgagcaa gcatgcatat cttcaatgac gatggttggt ttaccgggaa agagatccct
    31921 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
    31981 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
    32041 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccacg
    32101 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcactgac ggtgctgacc
    32161 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
    32221 gaaatgttag gcagcgtcgg tagcggattt attatgggca atgcgatgcc gcaactgcgc
    32281 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
    32341 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
    32401 cagcaccggg caattgcccg gttttttttg cgttgaattt gtcattttgt gccgtggtgt
    32461 ttaaaccgca cagaataaat tgtcgtgatt tcacctttaa aataaaatta aaagagaaaa
    32521 aaattctctg tggaagggct atgttagata aaattgaccg taagctgctg gccttactgc
    32581 agcaggattg caccctctct ttgcaggcac tggctgaagc cgttaatctg acaaccaccc
    32641 cttgctggaa gcgcctgaaa cggctggagg acgacggtat ccttatcggc aaagtcgccc
    32701 tgctggatcc ggaaaaaata ggcctcggcc tgaccgcctt tgtgctgata aaaacgcaac
    32761 atcacagcag cgaatggtat tgccgctttg tcacggtggt taccgaaatg ccagaagtgc
    32821 tggggttctg gcgcatggct ggtgaatacg attatctgat gcgcgtccag gttgccgaca
    32881 tgaaacgcta cgacgagttt tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg
    32941 tcacttccag cttcgcgatg gaacagatta aatacaccac ttctttaccc atcgaataaa
    33001 tatccagaat caagtcagga cacaacgcgt gcgattattt gctcaattaa gctggtattt
    33061 ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg cttgtcatta tcgcgatgct
    33121 gcaactggtt ccgccaaaag tggttggtat tgttgtcgat ggcgtaacag aacaacactt
    33181 tactaccggg cagatcctga tgtggatcgc caccatggtg ctgattgccg ttgtggttta
    33241 tctcctgcgt tacgtctggc gcgtattgct gtttggtgcg tcttatcaac tggctgttga
    33301 actgcgtgaa gattattacc gtcagctaag ccggcagcat cctgagtttt acctgcgtca
    33361 tcgcacgggt gacctcatgg ctcgtgcgac taatgacgtc gatcgcgtcg tgtttgccgc
    33421 cggagaaggg gtgctgacgc tggtggattc actggtaatg ggctgcgctg tgttgattat
    33481 gatgtctacg caaattagct ggcagttgac cttatttgcc ctgttaccga tgccagtgat
    33541 ggcgatcatg attaagcgca acggcgatgc tttgcatgaa cgctttaagc tggcacaggc
    33601 ggcgttttcc agtcttaatg accgcaccca ggaaagcctc accagtatcc gcatgatcaa
    33661 agcctttggt ctggaagatc gccagtcggc gttatttgcc gcggatgccg aagataccgg
    33721 caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc gacccgacca tctatatcgc
    33781 gattggtatg gcgaacttgc tggcgattgg cggcggtagt tggatggtgg tgcagggcag
    33841 tttaacgctg ggccagctca ccagttttat gatgtattta ggtctgatga tttggccaat
    33901 gctggcgctg gcatggatgt ttaacattgt ggaacgtggt agtgctgcgt acagccgtat
    33961 tcgcgcgatg ctggcggaag cgccggtggt gaacgatggt agcgaacccg tgccggaagg
    34021 gcgtggcgaa ctggatgtaa atattcacca gttcacgtat ccgcagactg accatcctgc
    34081 gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct gcgggccgac
    34141 tggttccggc aaaagtaccc tgttgtcgct cattcagcgt catttcgacg tcagcgaggg
    34201 ggatattcgc tttcatgata ttcctctgac gaagttacaa ctcgatagct ggcgtagccg
    34261 cctggcggta gttagccaga cgccattcct tttttctgac actgtggcga ataacatcgc
    34321 gctgggttgc ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt tagccagcgt
    34381 acatgacgat attttgcgtc taccgcaagg ttacgataca gaggtgggcg agcgcggtgt
    34441 gatgctttcc ggcgggcaaa aacagcgtat ctccattgct cgtgcgttat tagtcaacgc
    34501 ggaaatcctc atccttgatg atgcgctttc ggcggtggac ggacgcactg agcaccagat
    34561 cctgcataac ctgcgccagt ggggacaggg aagaacggta atcatcagtg cccatcgcct
    34621 ttcggcactg acggaagcca gtgaaattat tgtgatgcag cacggacata tcgcccagcg
    34681 tggcaatcat gatgtgctgg cacaacaaag cggctggtat cgcgatatgt atcgctatca
    34741 acaactggag gcggcgctcg acgacgctcc ggaaaatcgc gaggaggccg tcgatgcgta
    34801 gttttagcca actgtggccg actctcaagc gcctgttagc gtacggttca ccgtggcgta
    34861 aaccgctggg gattgcggtc ctgatgatgt gggttgcggc ggcggcagaa gtcagtgggc
    34921 cgctgcttat cagctatttt atcgacaata tggtagcgaa aaataacctg ccgttgaaag
    34981 tggttgcagg gctggctgcg gcgtatgttg ggctacaact gtttgccgcc gggctacatt
    35041 acgagcagtc tctgctgttt aatcgggcgg cagtaggcgt agtgcaacag ttgcgtaccg
    35101 acgtgatgga tgctgcgtta cgccagccat taagcgagtt tgatacccaa cccgtcgggc
    35161 aggtgatttc ccgcgtcact aatgacactg aagtgatccg cgatctctac gttaccgtag
    35221 tggcaactgt cctgcgcagc gccgcactgg tgggcgcgat gctggtggcg atgtttagtc
    35281 tcgactggcg aatggcgctg gtggcgataa tgattttccc ggtggtgctg gtggtaatgg
    35341 tgatatacca gcgttacagc acgccgattg tccgccgtgt gcgcgcctat ctggcggata
    35401 tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt tatccagcag ttccgtcagc
    35461 aggcgcgatt tggcgaacgt atgggtgagg ccagtcgttc acactatatg gcgaggatgc
    35521 aaaccctgcg cctcgacggt tttctgctgc gtccgctgct gagtctgttt tcatcgctca
    35581 ttctttgtgg cttgttgatg ctgtttggct tctccgccag cggcaccatt gaagtgggcg
    35641 tgttgtatgc gtttatcagc tatcttgggc gacttaacga acccttaatc gaactgacca
    35701 cgcagcaggc gatgctgcaa caggctgttg ttgctggtga gcgcgtgttt gagctgatgg
    35761 acggaccgcg ccagcaatat ggcaatgatg atcgcccgtt acagagtggc accatcgaag
    35821 tcgataacgt gtcatttgct tatcgcgata acaatctggt gctaaagaac attaatctct
    35881 ctgtgccttc gcgcaatttt gtggcgctgg tcgggcatac cggcagtggc aaaagcaccc
    35941 tcgccagttt attgatgggc tattacccgc taacggaagg tgagattcgc cttgatggtc
    36001 gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg cgtggcaatg gtgcagcaag
    36061 atccggtggt gctggcggat accttcctcg ccaacgtgac gctggggcgt gatatctccg
    36121 aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc ggagctggcg cgtagcatga
    36181 gcgacggtat ttacacgccg ctgggcgagc aggggaataa tctctctgtc gggcaaaagc
    36241 aactgctggc actggcacgc gtgttggtgg agacgccgca aatcctgatc cttgatgagg
    36301 caaccgccag cattgactcc ggtactgaac aggcgattca acatgctctg gcggcggtgc
    36361 gtgaacatac cacgctggtg gtgattgctc atcgcttatc gaccattgtt gatgccgaca
    36421 ccattctggt gcttcatcgt gggcaagccg tggagcaggg cactcaccag caactgctgg
    36481 cggcccaggg acgctactgg cagatgtatc aactgcaact tgcgggcgaa gagctggcag
    36541 ccagcgtgcg tgaagaggaa tcattgagcg cctgaatagc gcaatatttc atcgttggtg
    36601 caaaaatgta acgcactgtg cactgtcata gtgcgttttc attttcaaac ttcttaactt
    36661 cctgctctct ttctcgtttt tcatttctgg cacaccgctt gcaatacctt cttcgtgtag
    36721 cagaaccatt accgaattct gaccggaggg gatctatgaa gctggtgacc gtgataatca
    36781 aaccattcaa gctggaagac gttcgtgaag cgttatcttc cattggtatt cagggcctga
    36841 ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca tgccgagctg taccgggggg
    36901 cggaatacag cgtcaacttc ctgccaaaag taaaaattga tgtggcgatt gctgatgacc
    36961 aactcgatga agtgatcgat atcgtcagta aggcggctta caccggaaaa attggcgacg
    37021 gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat tcgtaccggc gaagccgacg
    37081 aagcggcgct gtaatctctg gcacacagca acaggaacga aaaatgaaga tagcgacgat
    37141 aaaaactggg cttgcttcac tggcgatgct gccgggactg gtaatggctg cacctgcggt
    37201 ggccgataaa gccgacaatg cgtttatgat gatttgtact gcgctggtgc tgtttatgac
    37261 tattccgggg attgccctgt tttacggtgg gttgattcgc ggcaaaaacg tgctgtcgat
    37321 gctgacgcag gtgacggtga catttgcact ggtctgtatt ctctgggtgg tttacggtta
    37381 ctcgctggcg tttggtgagg gcaacaactt cttcggcaac attaactggt tgatgctgaa
    37441 aaacatcgaa ctgacggcgg tgatgggcag catttatcag tatatccacg tggcgtttca
    37501 gggatcgttt gcctgcatta ccgtaggctt gatagttggg gcgctggcgg aacgaatccg
    37561 cttctcagct gtgttgattt tcgtggtggt atggctgacg ctctcttaca ttccgattgc
    37621 gcatatggtc tggggcggtg gtttgctggc ttctcacggt gcgctggatt tcgcgggtgg
    37681 caccgtggtg cacattaacg ccgcaatcgc cggtctggtg ggcgcgtatc tgataggaaa
    37741 acgcgtgggc ttcggtaaag aggcgtttaa accgcacaac ctgccgatgg tcttcaccgg
    37801 gactgccatt ctctatatcg gttggtttgg ctttaacgcc gggtcagcgg gcacggcgaa
    37861 tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca acggcggcgg caattcttgg
    37921 ctggatcttc ggtgaatggg cgctgcgtgg taagccttca ctgctgggag cgtgttctgg
    37981 cgcgattgcc ggtctggtcg gcgtgacgcc agcctgtggc tacattggtg ttggcggcgc
    38041 gttgattatc ggcgtggtag ctggtctggc gggcttgtgg ggcgttacca tgctcaaacg
    38101 cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg cacggcgttt gtggcattgt
    38161 cggctgtatc atgaccggga tttttgccgc cagctcgctg ggcggcgtgg gcttcgctga
    38221 aggtgtgacg atgggccatc agttgctggt acagttggaa agcattgcca ttacgattgt
    38281 gtggtctggt gtggtggcat ttatcggcta caaactggcg gatctgacgg ttggtctgcg
    38341 tgtgccggaa gaacaagagc gagaagggct ggacgtcaac agtcacggcg agaatgccta
    38401 taacgcgtaa caagcactgc aaaaaacagc cggacggttt tcacctccgg ctattttttt
    38461 aattgtgatt acgcatcacc ccttcctgaa cggtcgaggc aaccagtacg ccgtcctggg
    38521 tataaaactc accgcgcaca aagccacgtg cgctggacgc cgaggtgctc tccacgctat
    38581 acagcagcca ttcattcaaa ttaaacgggc gatggaacca catggaatgg tcaatggtgg
    38641 caatctgaat ccccggttcg agaaaaccga taccgtgcgg ctgtagagct accggcagga
    38701 agttaagatc agaagcgtaa ccgagcagat actgatgaac gcgcaggtca tccggcacgc
    38761 taccatttgc gcggatccac acctgacgat gtggttctgc gacgtgacct ttcagtgggt
    38821 tatgaaactc caccggacgg acttccagcg gacgatcgca gatgaattta tctttcagca
    38881 ctggcggcag caggtgcgcc agcgactggg cgatttgcgt ttccgaaggc aggccatcag
    38941 gcgctggcgc ggacggcatt gttttttgat gttcgaaacc cgcttctggt gcctggaaag
    39001 aggccgtcat ataaaaaatc ggtttgccgt tttgaatagc agcaacccgg cgggcgctga
    39061 agctgttacc gtcacgcagc gtttcgacat cataaataat cggcttctta ctatcgccag
    39121 ggcgaagaaa gtagctgtga aacgaatgta ccagccgctc ttcagggacg gtctcttttg
    39181 cagcatacaa ggcctgaccc acgacctggc cgccaaatac ctggcgtaaa cctaaatctt
    39241 cactctggcc gcgaaagagt ccttcctcaa ttttttccag atttaacaat gtcagtaaat
    39301 tttttagcgc ctgactcata taactctcca gtaacaaagc tgccgcagca agccaaagtg
    39361 agttgagtat aacgcaaatt tgctactggt ccgatgggtg caatggtctg aattacgggc
    39421 taattacagg cagaaatgcg tgatgtgtgc cacacttgtt gacgttacta ttttgttaac
    39481 cactcttccg gcgaggaaag ttagcccgct ggtgcattga taataaggag aaatgaatga
    39541 aactcgtgca catggccagt ggtttagcgg ttgcgattgc gttggcggct tgcgcagata
    39601 aaagcgcgga tattcagacg ccagccccgg ctgcaaatac gtctatttca gcaacacaac
    39661 aacctgctat ccagcaaccg aatgtctccg gtaccgtctg gatccgtcag aaagtcgcac
    39721 tgccgcctga tgctgtgctg accgtgacac tttctgacgc gtcgttagcc gatgcaccgt
    39781 caaaagtgct ggcgcagaaa gcggtgcgta ctgaaggtaa acagtcacca ttcagctttg
    39841 ttctgccatt taacccggca gatgttcagc cgaacgcgcg tattctgttg agtgcggcga
    39901 ttaccgtgaa tgacaaactg gtatttatca ccgataccgt tcagccggtg atcaaccagg
    39961 gcggaactaa agccgacctt acattggtgc cggtacagca aaccgccgtg ccggttcagg
    40021 ccagcggtgg cgcaacgact accgtacctt cgacttcacc aactcaggtg aatccgtctt
    40081 cggcagttcc agctcctacg caatattaag cgacgttaac cctctccgcc cggagagggt
    40141 tagtagttcc agcgataacg ctgcaagtcg atttgcccgc ttcccgatac catcacacct
    40201 tctgccagta atgcctgtcg ctgacgctgt aaatccggtc cggttagcga aattgtgccg
    40261 tggcgattaa ccacccggtg ccagggtaag gtgctgcctt cagggagacg ctttaacaca
    40321 ccgcccacct ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc accgtaagtg
    40381 gtgacatagc cttcgggaat agcggcgacg atttgccaga cgcgttgggg aaatgaatct
    40441 tctttttcca tcttttcttc ctgaggtaat ttttcagcat aatctggaaa aacgcccgag
    40501 ttaagtcgca ttgcgcaaga aaccagcatc tggcacgcaa tgggttgcaa ttagccgggg
    40561 cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt
    40621 tctcccgcaa cgctactctg tttaccaggt caggtccgga aggaagcagc caaggcagat
    40681 gacgcgtgtg ccgggatgta gctggcaggg cccccaccca tttctgcctc ccaccgtttc
    40741 gtcaaaaaat cccaacatgg ctaaacttta accacaactg acgtcgcaag aattgtctgg
    40801 ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt tgatggtttt gtggttgccg
    40861 ttcctgccga taaaaaggat gcctatcggg aaatggccgc taaggccgcg ccattgttta
    40921 aagagtttgg cgcgcttcgt attgtcgaat gctgggccag cgatgtaccg gatggcaaag
    40981 tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga agaggttgtc tttagctgga
    41041 ttgaataccc ttcaaaagag gtccgcgacg cagctaatca aaagatgatg tcggacccac
    41101 ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa gcgaatgatc tatggcggat
    41161 tcgagtcaat catcgacgaa taggtagcgt gacgggctgt gcggcgtgcc gtcagcccaa
    41221 aaattcacaa atgttgctcg gcccagcgta agaaatcttc tttcggtaat gccttgctgt
    41281 agagccagcc ctgaccgtag tgcacgccat gctggcgtag ccactcttct tgtttactgg
    41341 tttcgattcc ctccgctacc attttcagtt tcagtgtttt tgccatttcg atgatatgcg
    41401 gcgtgacatt tttatattcc agcgcatcaa cgaaagattt atcgatcttc agaatgtcga
    41461 catccagatc ctgtaaataa cttaaacttg aataccccgt accaaaatca tcaagataaa
    41521 tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc gctggttttc ggatcggcaa
    41581 actcgcgttc agtgagttca agcgcgatct gtctgggatt aacctgatag tgattgatca
    41641 tttcacgcag caattgcggg attttttccg aggtgagcac ggtggattca agattgatcg
    41701 aaatatgctg ctgtggatgc tgacgcagcc agtcgcccat atcttcaaag acgcttctta
    41761 taatcagtag cgtcaatggc tcagaaaggc ccgtttgctg tgccagcgga ataaaactat
    41821 ctggtgacaa ccaactaccg tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa
    41881 ttttgccatt ggctaaggag acaatcggct gatagtgcac gcaaatatca cgattttcga
    41941 tagcatcctg cagccgatga tgcggtgact ggatgcggcg caaaatacgc agcacaaaca
    42001 tcgctgccag caggccaatc accaacccga gcggtaacca aataaagact tgccgatgcc
    42061 agcctttctg caacattttc gttgaagccc aggtgatgat cgaaatattc atctccggga
    42121 agggcaggat atcgtaaatg attccattat tttcgatatg ctcaccgggt gttttttgta
    42181 gcctggtaat aattccctga gcaatttcat cgctgctggt tatgacaaca ttatgggcat
    42241 tgccaataat ggcggcatca atttgccatg agctataggg aatgacatca ataaaggaag
    42301 cggggtcgat catgacgaca taatgtgccg ttcccatggc gaccatgtaa cggataatgc
    42361 ctaaatcgtt atgcgatgtt aaccagacac gataaccatc tttcgaaatt ttacctggct
    42421 cggggaaggt atcgggcgga ctctcatgct ccagagacga acattgggga acgttgttat
    42481 cgatataaac cacttcctga atatagcgat aactgtaaga tacccgacgc atttccatga
    42541 gatgggcttc gctacaggca gcgccttgcc atctttccag ctcttgcagc gcatctttcc
    42601 cttgtgtcgc caccttattg gcccgaatag cgacgcggga ggaataggta tccagctctt
    42661 caataaacga tgtttctacc tgctgatggg ccagccagat gcttaagccg acaggcagca
    42721 atactgaaag aatcagtact cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca
    42781 tatccttatt aacacagtgt agggaaagta tatctgattg tggaaaaagg ctggcaggag
    42841 ataaggaggt agagctggta tttcatgaag tgatgggtgg aaacgatcgt tgtcatttga
    42901 ccaggcgcag agctttacgc ctggtatgta atcctaactt aagatgttga ttcggcgcga
    42961 gcgagggtaa aaatatcgta aaaagacagt tcacctttac ggacgatcat tttttgcagt
    43021 tgttccttat gaacatccgc gacacgcgga gagttcaaga tggcttcaac cagcgcggca
    43081 ggtacaaagc gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg
    43141 atatcttcat agcgatagcg tgggtagaca tcgaaaaaga agaaaccatt aagcaggata
    43201 aataatacaa ccagtagcca gacagagcct gaaagcgttt cggactgcaa catcaccgcc
    43261 agggtggcaa aaaaagcaac gtacataccg ataaacagcc ccggatgttt acggataaaa
    43321 ctgatactaa agcggggttt gttgtcgcgt ttttcgcggg tattcagaga ctcaatcgtt
    43381 tcggtcagca ggcgttgtat ttcggtcatc gtttgccttc gtggtatctg caactttcca
    43441 gggaacaccc tattttaacg tgggaatagt agagggaaag taacagattt accacaattt
    43501 tgcataacag ttgcgaaaaa ccgattacaa ttttttaatt attctggctg gattaccgcc
    43561 cacgacaacg ttgtccggga catcttttgt gacaactgca cctgaggcta ccacgacgtt
    43621 atcaccaatg gtcacaccag ggttaatgac cgcgcgtccg ccaatccaga cgttattacc
    43681 gatggtgacg ggtttcccca gttcagcacc gctattacgt gctacagggt cgatgggatg
    43741 tgttgccgtg tagatatgaa cgcctggtgc caacatacag ttatcaccga tgcgaatagg
    43801 gcagacatca agcatcacgc aatcgaagtt ggcgaaaaaa ttattaccga gaaaaatgtt
    43861 atagccatag tcacagcgaa acgttggctc aatataagcc tctgtcacct gaccgaataa
    43921 atcagcgaga atttgctggc gtaatgtgtg ctcttccgcc agggaatgat tgtatcggtg
    43981 aataagctga cgagcgcgca ggcgatcgcg agataacgtc tcatctgccg agcgatacaa
    44041 ctcaccagca atcatctttt ctttttctgt gctcattact gacctccggt tgctgaaaat
    44101 gcggcaacac agtaagacga aaagtcagtg aagagaacgt tcccattaca tctgtgtgat
    44161 aaagatcaca tagggtatgt cttcatggcg aaaaagtata aaattcttaa taaacagccg
    44221 gttatagctc cgaaagcgaa ttatttatta gcgaataaat ttccatactg aggaagggat
    44281 cttgtcgtac agtttattca tggtcaattc ggcgaggcgg tgatctgcgg ctgagtaaaa
    44341 taccgccagt tcattatctg ataattcgta tttatttttc tcgataacac gctccagcgt
    44401 gtcaattgtc tggcaacgac gtaaacgcat taaataatcg gttttcgtta aaggtttttc
    44461 ggacatactt ctacccatgg ttgtaataat tctaacaaga taaactcgca ggattctctt
    44521 tcgtcgcatt gacaaaacaa cggaatagtc gattacctga cttccgccat ttctgaagat
    44581 cctgcatatt aataccataa ctgctgaaca acataaaggt gtcatccaga tattcgtcga
    44641 tctgctcaat gagcttattg tcttcattat acttaatttt gtaattaagt gcgaaggtcg
    44701 caatatgctc aatcagttca ttcaactgga ggttgatcgc cgaggttggg tcgtttaccc
    44761 agccatgatt gctttcttca aggtttgcaa ggcagtcatg atacagggtt tcacagagaa
    44821 acttaagctg tgcgatatca tgtcttttgg gtgagtattc atccataacg cgtccccttc
    44881 ttagcggttg aactaacgga cacctttcgg gatggaaaaa acttactgac ctggacttgc
    44941 cctctcgttt gttagcttaa ctataagcca ctctttgcag gttttcatcg cattattacg
    45001 aaaaattaca attaggaagg aagcgggaca ggactcactt tttcatacta gcactaaccc
    45061 gcagcaggta aaagcagttt gctcgaacta ttcataacat ttcaggatta tcgccagatg
    45121 aaattggtga cactaatacc aggattgctc tgaatatgac gtaataaccg aggaatgaat
    45181 aaagaattac cgcaaataat taagaatagc ctct
//
LOCUS       DAPGRN010000026        44370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPGRN010000026 DAPGRN010000000
VERSION     DAPGRN010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.026"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(73..1356)
                     /locus_tag="RHW33_001576"
     CDS             complement(73..1356)
                     /locus_tag="RHW33_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV1645096.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFTMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1469..3403)
                     /gene="yeaG"
                     /locus_tag="RHW33_001577"
     CDS             complement(1469..3403)
                     /gene="yeaG"
                     /locus_tag="RHW33_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV1645097.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3839..4585
                     /gene="mipA"
                     /locus_tag="RHW33_001578"
     CDS             3839..4585
                     /gene="mipA"
                     /locus_tag="RHW33_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV1645098.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4675..5529
                     /gene="yeaE"
                     /locus_tag="RHW33_001579"
     CDS             4675..5529
                     /gene="yeaE"
                     /locus_tag="RHW33_001579"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HDV1645099.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASKCKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELAMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5580..6464)
                     /locus_tag="RHW33_001580"
     CDS             complement(5580..6464)
                     /locus_tag="RHW33_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV1645100.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAERGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6548..7543)
                     /gene="gapA"
                     /locus_tag="RHW33_001581"
     CDS             complement(6548..7543)
                     /gene="gapA"
                     /locus_tag="RHW33_001581"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV1645101.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7885..8298
                     /gene="msrB"
                     /locus_tag="RHW33_001582"
     CDS             7885..8298
                     /gene="msrB"
                     /locus_tag="RHW33_001582"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV1645102.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8340..8612
                     /locus_tag="RHW33_001583"
     CDS             8340..8612
                     /locus_tag="RHW33_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV1645103.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARNNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8982..10058
                     /locus_tag="RHW33_001584"
     CDS             8982..10058
                     /locus_tag="RHW33_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV1645104.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNAWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10085..11464
                     /locus_tag="RHW33_001585"
     CDS             10085..11464
                     /locus_tag="RHW33_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645105.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRHTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFIFLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11481..12524
                     /locus_tag="RHW33_001586"
     CDS             11481..12524
                     /locus_tag="RHW33_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDV1645106.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12545..13381
                     /locus_tag="RHW33_001587"
     CDS             12545..13381
                     /locus_tag="RHW33_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDV1645107.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEVNIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            13386..14333
                     /locus_tag="RHW33_001588"
     CDS             13386..14333
                     /locus_tag="RHW33_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HDV1645108.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVNIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            14343..15323
                     /gene="ydjG"
                     /locus_tag="RHW33_001589"
     CDS             14343..15323
                     /gene="ydjG"
                     /locus_tag="RHW33_001589"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HDV1645109.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREEVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFYTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPQQVRENVAALNINLSDADATLMREMAEALER"
     gene            15460..16218
                     /locus_tag="RHW33_001590"
     CDS             15460..16218
                     /locus_tag="RHW33_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1645110.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16336..17694
                     /locus_tag="RHW33_001591"
     CDS             16336..17694
                     /locus_tag="RHW33_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645111.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPAMVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFMLGGIGILLAWLLSGKYFIESPRWLAGKGQIAGAECQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17787..18428)
                     /gene="pncA"
                     /locus_tag="RHW33_001592"
     CDS             complement(17787..18428)
                     /gene="pncA"
                     /locus_tag="RHW33_001592"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV1645112.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(18439..19455)
                     /gene="ansA"
                     /locus_tag="RHW33_001593"
     CDS             complement(18439..19455)
                     /gene="ansA"
                     /locus_tag="RHW33_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV1645113.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19609..21465)
                     /gene="sppA"
                     /locus_tag="RHW33_001594"
     CDS             complement(19609..21465)
                     /gene="sppA"
                     /locus_tag="RHW33_001594"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV1645114.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            21626..22177
                     /locus_tag="RHW33_001595"
     CDS             21626..22177
                     /locus_tag="RHW33_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV1645115.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22294..23337
                     /gene="selD"
                     /locus_tag="RHW33_001596"
     CDS             22294..23337
                     /gene="selD"
                     /locus_tag="RHW33_001596"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV1645116.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            23342..25303
                     /gene="topB"
                     /locus_tag="RHW33_001597"
     CDS             23342..25303
                     /gene="topB"
                     /locus_tag="RHW33_001597"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV1645117.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            25430..26470
                     /locus_tag="RHW33_001598"
     CDS             25430..26470
                     /locus_tag="RHW33_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV1645118.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKSIEPLSIAKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(26587..27930)
                     /gene="gdhA"
                     /locus_tag="RHW33_001599"
     CDS             complement(26587..27930)
                     /gene="gdhA"
                     /locus_tag="RHW33_001599"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV1645119.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            28166..28438
                     /locus_tag="RHW33_001600"
     CDS             28166..28438
                     /locus_tag="RHW33_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HDV1645120.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(28404..28811)
                     /gene="nudG"
                     /locus_tag="RHW33_001601"
     CDS             complement(28404..28811)
                     /gene="nudG"
                     /locus_tag="RHW33_001601"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV1645121.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            28898..29518
                     /locus_tag="RHW33_001602"
     CDS             28898..29518
                     /locus_tag="RHW33_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV1645122.1"
                     /translation="MLDRHLHPRIKPLLHQCVRALDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(29527..30834)
                     /gene="ynjE"
                     /locus_tag="RHW33_001603"
     CDS             complement(29527..30834)
                     /gene="ynjE"
                     /locus_tag="RHW33_001603"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV1645123.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(30901..31554)
                     /locus_tag="RHW33_001604"
     CDS             complement(30901..31554)
                     /locus_tag="RHW33_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV1645124.1"
                     /translation="MLCVKNVSLRLPESCLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(31554..33089)
                     /locus_tag="RHW33_001605"
     CDS             complement(31554..33089)
                     /locus_tag="RHW33_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1645125.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCTRLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGFTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDSDQQTKGALASLLLMLLLAAYVLLGYLLWCTWRRTIPRVDGVRKP
                     ATPLLPGITLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLTATFIALILIL
                     AWLECGSSRRHFWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWIL
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLILRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(33062..34228)
                     /locus_tag="RHW33_001606"
     CDS             complement(33062..34228)
                     /locus_tag="RHW33_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1645126.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTDWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSILDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(34238..34786)
                     /locus_tag="RHW33_001607"
     CDS             complement(34238..34786)
                     /locus_tag="RHW33_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV1645127.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLTTHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(34786..35493)
                     /locus_tag="RHW33_001608"
     CDS             complement(34786..35493)
                     /locus_tag="RHW33_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV1645128.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSAMAGAALCFFIARVLGREVVEKLTGKTVLNSMDGFFTRYGKHTILVCRLLPFVPF
                     DPVSYAAGLTSIRFRQFIIATGIGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(35508..36185)
                     /gene="ydjY"
                     /locus_tag="RHW33_001609"
     CDS             complement(35508..36185)
                     /gene="ydjY"
                     /locus_tag="RHW33_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HDV1645129.1"
                     /translation="MLQHYSVSWKKGLTALCLLAVAGLSGCDQKENTAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKTYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(36190..36900)
                     /locus_tag="RHW33_001610"
     CDS             complement(36190..36900)
                     /locus_tag="RHW33_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV1645130.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSMLVIAGGIVFGPFLGTLLSLIAATLASSCSFLLARW
                     MGRDLLLKYVGHSHTFKAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTAIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRNPLTHPKNEG"
     gene            complement(37067..37873)
                     /gene="xthA"
                     /locus_tag="RHW33_001611"
     CDS             complement(37067..37873)
                     /gene="xthA"
                     /locus_tag="RHW33_001611"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV1645131.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            38319..39539
                     /gene="astC"
                     /locus_tag="RHW33_001612"
     CDS             38319..39539
                     /gene="astC"
                     /locus_tag="RHW33_001612"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV1645132.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNIINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACERFVSGGSS"
     gene            39536..40570
                     /gene="astA"
                     /locus_tag="RHW33_001613"
     CDS             39536..40570
                     /gene="astA"
                     /locus_tag="RHW33_001613"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV1645133.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTTPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            40567..42045
                     /gene="astD"
                     /locus_tag="RHW33_001614"
     CDS             40567..42045
                     /gene="astD"
                     /locus_tag="RHW33_001614"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV1645134.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAALVGQACR
                     AARAAFPRWARLSFAERQAVVERFAALLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTCEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPHLLQAGTSLLTPGIIEMTCVAGVPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFEQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            42042..43385
                     /gene="astB"
                     /locus_tag="RHW33_001615"
     CDS             42042..43385
                     /gene="astB"
                     /locus_tag="RHW33_001615"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV1645135.1"
                     /translation="MNAWEVNFDGLVGLTHNYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQAQLLAN
                     LRARVNSFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            43378..44346
                     /gene="astE"
                     /locus_tag="RHW33_001616"
     CDS             43378..44346
                     /gene="astE"
                     /locus_tag="RHW33_001616"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV1645136.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT        10876 a        10805 c        11674 g        11015 t
ORIGIN      
        1 atgtatgtaa taaatgagat aatttacagt gagctaaaaa tcagccaggc gataatgacc
       61 cggctgatag ttttagtctt tagctgttgc attttgttta tgaaacagtt cacggaacac
      121 cggataaata tcatcctggt cgcggatgtg ctgcatcgta aagttgtcga aagtagattg
      181 cagatgctca tattctcgcc acaatgtctg atgtgcacga cgagtaattt cgatatagct
      241 gtaataacga acaacaggta ataatttttt cgccaggatt tcatggcaaa gcggagagtc
      301 atcggcccag ttatcgccgt ccgatgcttg tgcagcgtaa atattccact gtgccgggtt
      361 ataacgctct ttcactacct catccatcag tttcagggcg ctggaaacaa tggtgccgcc
      421 agtttcctgc gagtagaaaa actcatgttc atcgacttct ttcgcctggg tatgatggcg
      481 gatgtatacg acttccacgt tcttatacgt tctgctgagg aacagataca gcagaatata
      541 aaaacgctta gccatatctt tagtggattg atccattgaa ccggaaacgt ccatcaggca
      601 aaacatcact gcctggctgg agggatcggg ccgcttctcg tagttcttgt aacgtaaatc
      661 gaaggtgtca ataaaaggga cgcgttcaat tttggcacgt aattctgcaa tttctttgcg
      721 cagacgttcc tcttccagca gctgcgcagg ttcactgttg ctgatgatgg ccaaattctc
      781 ttccagtgca tgaagttccc gccgcttgcc tgccgtcatg gctgtgcgtc gcgccagtga
      841 gttctgcaat gaacgcacaa cgctgatatt ggccggaacg ccgttagcgg tataacccgc
      901 ccgatgcgtt ttatattcgg tcagctggcg ttgttggttt tgtttcagat tcggtaaggc
      961 caaatcttca aagagcagat caagatactc atctttcgaa atctgaaaga cgaattcatc
     1021 ctgaccttca ccatcctggc tggcctggcc ctgaccactg ccggaacctc cgccgccacc
     1081 ctggggacgt tcaattcggt cgttctggac gaaatggtca ttgcccggat gtacgcggtg
     1141 gcgcaggccg ccacgcccct gatgaaacat cggttcgcta atatcttccg tgggaatgga
     1201 tacggattcg ccgctgtcga cgtcagtcac cgaacgctta ttaatggcct cggagatcga
     1261 ctgtttaatt tgcgctttat aacggcgtaa aaaacgctgg cgattcacca tgcttttgtt
     1321 tttgccgttc agacgccggt caataaacca ggtcatatgc cccccgtact acatttgcca
     1381 actgtatgct acgcctgtgt tgccatttct ctggcccacg gttcgctgtc gttgtaggcc
     1441 cggtagcatg tgtaccgggc cagggttgtt aagacgattt acgtacgcgc aaataccatt
     1501 cgcacagtaa acgcacctgt ttacgggtgt agcctttttc catcatacgg tcgacaaagt
     1561 cgtcgtgttt cttctgctcg tcggttgacg ttttggcgtt aaacgagata accggcaaca
     1621 gctcctcggt attggagaac attttcttct cgataaccgt gcgcagtttt tcatagctgg
     1681 tccagttcgg attgcgtccg ctgttattgg ctctggcgcg cagtacgaag ttaacaatct
     1741 cgttgcggaa atcttttgga ttactgatcc ccgccggttt ctcgattttc tccagctcgg
     1801 cgttaagaga ctcgcggtca aacagctgcc cggtatccgg atcgcgatac tcctgatcct
     1861 gaatccagaa atccgcgtag gtaacataac ggtcgaaaat gttttgccca tattcggaat
     1921 aggattcaag gtaggccgtc tggatctctt tgccaataaa ctcggcatat ttcgggatca
     1981 gataaccttt caggaactcc agatagcgtt ctgcctgctc ttgtgggaac tgctcgcgct
     2041 cgatctgctg ttccaggacg tagaacagat ggaccgggtt tgccgccact tctacatgat
     2101 cgaagttgaa cacgcgggag aggatcttaa acgcaaaacg cgtcgacaga ccgttcatcc
     2161 cttcatcgac accggcgtag tcacgatatt cctgatacga cttggctttg ggatcggtgt
     2221 ctttcagact ttcgccatca taaacccgca tctttgaata aatgctggag ttttctggct
     2281 ctttcaggcg cgaaagaatg gaaaaacgtg acagtgtttc gagcgtgcca ggggcgcatg
     2341 gggcgtgagt caattcactg tgattaagca atttctcgta gattttgatc tcttcggaaa
     2401 tgcgcaagca atacggcacc ttcacgatgt aaacacgatc gaggaaggct tcgttgtttt
     2461 tgttattacg gaaagtgacc cattcggact cgttcgagtg ggcaagaata atcccgttga
     2521 acggcagggc ggagatacct tccgtcccgt tgtagttacc ttcctgagtg gcggttaaca
     2581 agggatgcag cactttaatc ggtgctttaa acatctcaac gaattccatg atcccctggt
     2641 tggcgcggca cagtgcgccg gaatagccat aggcgtctgg gtcattctgc gcgtagtgtt
     2701 cgagtttacg aatatcgact ttccctacca gcgcggagat gtcctggttg ttctcatcac
     2761 cgggctctgt tttggcgata gcaatttgtt gcagaatgga cggccagacc ttcactaccc
     2821 ggaacttagt aatatcgcca ccaaattcat gcagacgttt tgccgcccac ggcgacatga
     2881 tggtgccgag ataacggcga ggaatgccat actctttttc cagaatctgc gcatcttcct
     2941 gcggattgaa aagacagaac ggatgatcgt tgaccgggct acgctcaccg ttggcgctca
     3001 atacataaat cggtacgagc tgcattaatg atttcagtcg ctcagcaagc gatgatttac
     3061 ccccacccac aggccccagc agatacagga tttgtttctt ctcttccagc ccctgagccg
     3121 cgtgtttcag ataagagaca atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa
     3181 acgccggata acgtgcaatg acccggttag aaaagagtcg agaaagtctg ggttcctggg
     3241 ctgtatcgac catgacaggc tcaccgatag ccatcaatag ccgctcagcc gcgttggcat
     3301 aagcactgcg atcttgccga caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct
     3361 tggcagcttc atatcgctgg cgatagtgat cgaatatatt catggtatgc cgtcctttcg
     3421 ttttttagca caggttaaga gccgttcata tgaatagtag aggctcccgg aagaggtaaa
     3481 ctgaacgacg tctcttgcta aaacacagca actctcatgc cattttatcg acgatgagaa
     3541 caacgtgccc ggtgatacac cttcaaaatt aagcgtagat ggcaaatgga aaacttgcct
     3601 acacggcttt cttaatttca atggcatatc aacaactcat ccataaattt tgcataatta
     3661 atgtaaagac caggctcgcc agtaacgcta aattcatttg gctgtaagcg cggtgtcatc
     3721 cgcgtcagga aaattaaaca gttactttaa aaaatgaaaa cgtaaaaagg ttgggtttcg
     3781 atgtattgac gggtaaactt tgtcgcccgc taaacatttg tttaaggaat gattaattgt
     3841 gaccaaactc aaacttctgg cacttggagt gcttatcgca acgtctgcag gcgtagcgca
     3901 cgctgaaggt aaattttccc tgggcgcagg cgtaggtgtc gttgaacacc catataaaga
     3961 ttacgatacc gatgtttacc cagtaccggt aatcaactat gaaggcgata acttctggtt
     4021 ccgtggctta ggtggtggtt actacctgtg gaatgacgca acggataaac tttcaattac
     4081 cgcttactgg tcgccgcttt acttcaaagc taaagacagt ggcgatcacc aaatgcgtca
     4141 cctggatgac cgtaagagca ccatgatggc tggtctgtct tatgctcact ttacccagta
     4201 cggttacctg cgtaccaccc tggctggcga taccctggat aacagcaacg gcatcgtctg
     4261 ggatatggcc tggttgtatc gttacaccaa cggcggcctg accgtgactc cgggtattgg
     4321 tgtgcagtgg aacagcgaaa accagaacga atactattat ggcgtatcgc gcaaagagtc
     4381 cgctcgcagc ggtctgcgtg gctataaccc gaacgacagc tggagccctt acttggagct
     4441 gagcgccagc tacaacttcc tcggcgactg gagtgtttac ggtaccgcgc gctacacccg
     4501 tctgtctgat gaagttactg acagcccgat ggtggataaa tcctggactg gcctgatttc
     4561 taccgggatc acctacaaat tctgataatg catctttcca gggcattttg tgcataaaaa
     4621 cagggcgtca cgccctgttt tgcattacgg tggtgcaata cctgggagga aacaatgcaa
     4681 caaaaaatga ttcaatttag tggcgatgtc tcactgccag ccatagggca gggaacatgg
     4741 tatatgggcg aagatgccag taagtgtaaa acagaagttg ctgcactacg cgcgggcatt
     4801 gagctcggtt taaccctcat tgataccgcc gaaatgtatg ccgatggtgg tgccgaaaag
     4861 gtggttgggg aagcattaac cggtctgcgt gataacgtct ttctcgtctc taaagtctat
     4921 ccgtggaatg ctggcgggca aaaagcgata aatgcatgcg aagccagttt acgccgtctc
     4981 aatactgatt atctcgatct ctacttatta cactggtctg gcagtttcgc ttttgaagag
     5041 actgtcgcag cgatggaaaa attgatcgcc cagggaaaaa tccgccgctg gggcgtttcg
     5101 aaccttgatt atgctgatat gcaggaactc tggcagctgc cggggggaaa tcagtgtgcc
     5161 actaatcagg tgctttacca tctcggttca cgagggattg agtacgatct actcccctgg
     5221 tgccagcaac agcagatgcc ggtgatggct tacagtccgt tagcccaggc cgggcggttg
     5281 cgcaatggac tgttaaaaaa cgcggtagtc aacgaaattg cacatgctca caatatcagc
     5341 gcggcacaag tattgttggc gtgggtaatc agccatcagg gtgtgatggc gattccaaaa
     5401 gcggccacga ttgcccatgt ccaacagaat gcggctgtgc ttgaggtcga actttcgtca
     5461 gcggaattag ccatgctgga caaagcatat ccggcaccaa aaggaaaaac tgcgctggat
     5521 atggtttgat gttgatacgt gccggatgca tcgcacccgg cacacaacat taacgtaaat
     5581 taacgtttcg caacgcgaat ggattgcgcc agatgtgcag gtttttcttt ggtcactttt
     5641 tgcgtttctg aagcgtaagc cgtttctaca caaacaaagg tcttgtagcc atcgtccggc
     5701 atatcgccca tgctaactga aagcgccggt cccgggttcc agcctacaac gttcagatga
     5761 tgctggtggc ctacggcgat aatacgattc agcgcttcat cattaatcac gctgcaatct
     5821 tgcggattca gatacacgcg atcggtacgg tcagggaagg tctgaatacc gtcggtcagt
     5881 acatcttctt tcgcgtcatt tactttatca atgaagcgat cgcccagccc actgacgctt
     5941 accttagcga tatcacccac gttaaagtag gtatgcaggg cagaggtggt ttcaaattcg
     6001 ccgtgtgctt caagatcgat ttcacaggtt ttacccacgc ggaaatgcgc taacagcgta
     6061 aagtcgtgag gccagaattt tttcgtctct tcgctttgtg tcaattcaaa agtcagcgct
     6121 acgccatcag catcttcacg atgtgatttc agcgtccacg gcaggttgcg ggcaaaaccg
     6181 tgcgcaggca ggcctcgttc tgccgccgga ccaaaccacg gccagcaaac cggtacgcca
     6241 ccgcgaatgg cgacgccatt tttgaacggt gtgttgttgc tcaaccacag aacttcttct
     6301 tcacccgcag gtttccacga gagaaggtgt gcgccctgta atgcaaaaga ggcttttacc
     6361 tggggatgat cgactacaat gaggtccagt tcatccagtt tacgacggga gaggacaggg
     6421 gagatttgtt cgatgaccgg aagggcaaaa attttcttaa tcatgacgca gtcctttaac
     6481 ttcattttat caggtaaaaa aaagagcgac cgaagtcgct ctttttagat cacagtgtca
     6541 tctcaactta tttggagatg tgagcgatca ggtccagaac tttgttggag taaccggttt
     6601 cgttgtcgta ccaggatacc agtttcacga agttgtcgtt cagagcgata ccagctttag
     6661 catcgaacac ggaagtgcaa acttcgccgt tgaaatcggt agatactacg tcatcttcgg
     6721 tgtagcccag aacgcctttc atttcgcctt cagcagcagc tttaacggca gctttgatct
     6781 gctcgtaagt tgcagctttt tccagacgaa cggtcaggtc aactacagat acgttcgggg
     6841 tcggaacgcg gaacgccata ccagtcagtt tgccattcag ttctggcagt actttaccta
     6901 cagctttagc agcaccggta gaggacggga tgatgttctg ggaagcgccg cggccgccgc
     6961 gccagtcttt gtgagacggg ccatcaacgg ttttctgagt agcggtagta gcgtgaacgg
     7021 tggtcatcag accttcgatg atgccgaagt tatcgttgat aactttagcc agcggagcca
     7081 ggcagttggt ggtgcaggaa gcgttggaaa cgatgtcctg gccagcatat ttgtcgaagt
     7141 tagcgccttt aacgaacatc ggagtgttgt ctttagacgg accagtcata accactttct
     7201 tcgcaccagc ggtgatgtgt ttacgagcag tttcgtcagt caggaacaga ccagttgctt
     7261 cagcgacaac gtcaacacca acttcgtccc atttcaggtt agccggatca cgttcagcgg
     7321 taacacggat ttttttaccg ttaacgatca gatgaccgtc tttcacttca acggtaccgt
     7381 cgaaacggcc gtgagtggag tcatatttca gcatgtatgc catgtaatca gcgtctaaca
     7441 ggtcgttgat tgcaacgatc tcgatgtcag aacgtttctg agcagcacgg aaaacaatgc
     7501 gaccgatacg gccaaaaccg ttgataccta ctttgatagt catatattcc accagctatt
     7561 tgttagtgaa taaaaggttg cctgtaaaat tacaaaaacc ttacgcagcg tcaagcggaa
     7621 tcgtgtcaat cattgcgaca aatcaatcct gtgcctaagc ataacgcgac tgactcgcct
     7681 cactcttcct ttgggcttga gaccacatgg ggtcggcgcc ccgaatttta aagggcaatt
     7741 acgataaaaa tgtgattttc atcacgattt aggtgcagcc atttcgtcga gatcaagttt
     7801 aggacaaaat tttgccagtt ggtgttaatg ttttgttaga atcagtcacg taatgtgagc
     7861 acgattaaag tgagatgtga gcaaatggct aataaacctt cggcagaaga actgaaaaaa
     7921 aatttgtccg agatgcagtt ttacgtgacg cagaatcatg ggacagagcc gccatttacg
     7981 ggtcgtttac tgcataacaa gcgtgacggc gtatatcact gtttgatctg cgatgccccg
     8041 ctgtttcatt cccaaaccaa gtatgattcc ggctgtggct ggcccagttt ctacgaaccg
     8101 gtgagtgaag aatccattcg ttatatcaaa gacttgtcac atggaatgca gcgcatagaa
     8161 attcgttgcg gtaactgtga tgcccatctg gggcatgtct tccccgacgg gccgcagcca
     8221 acgggcgaac gttattgtgt taactctgct tctttacgct tcactgatgg cgaaaacggc
     8281 gaagaaatca atggttgaaa aaacgattca gcaacttatt ccacaggagc ggaaaaatta
     8341 tgaatcttga tgacattatc aacagcatga cgcctgaggt ataccagcgt ttgtcgaccg
     8401 ccgttgaact ggggaaatgg cctgatggcg ttgcgttgac cgaggaacaa aaagagaact
     8461 gcctgcaact ggtgatgctg tggcaagccc gcaataatac cgaagcacag catatgacaa
     8521 ttgacacaaa tggtcagatg gtcatgaaga gcaaacaaca gttaaaagaa gattttggta
     8581 tctcagcaaa gcctattgcc atgtttaagt aagtggtttg tccccttttt gccgcggtta
     8641 taaaggggat agccaacaat aaccccacga ctcatctcaa ttatctcgtc acgaccaggc
     8701 aaagccccag cccggtctct tgccgtcgcg ttcacgcctg aatgacttct cctgaactta
     8761 agtctcagac ctatttggcc ggtaatccct ctcaaatttg ctcattttgc gttccgcctt
     8821 ccatcaacct gtaacagaac cagccatttt ctgcgtaatt gtgatctgag ttgtaaaacg
     8881 cagatcatta tctgtgtttt acagatttaa tgttgacctc aaaaatcatg tgggtaagta
     8941 tgatgaccat ctaagcaaaa caaaacacag aggatgacgc aatgaaagca ctggctcggt
     9001 ttggcaaagc ctttggcggc tacaagatga tcgatgtgcc acaacccatt tgtggcccgg
     9061 aagatgtagt gattgaaatt aaagccgcgg caatctgcgg cgcagacatg aagcactaca
     9121 atgtcgatag cggttctgat gagtttaact ctatccgcgg ccatgagttc gcaggttgta
     9181 ttgcgcaggt tggtgaaaaa gtcaaagact ggaaagtggg gcaacgcgtc gtgtcggata
     9241 acagcggtca cgtctgcggc gtttgtccgg cctgtgaaca aggtgatttt ctgtgttgta
     9301 cagaaaaagt aaaccttggt ctggataata atgcctgggg cggtggtttt tccaaatatt
     9361 gtctggttcc tggtgaaata ctcaaaattc atcgtcatgc gttgtgggaa atccctgatg
     9421 gtgttgatta tgaggacgca gccgtacttg accctatctg taatgcctac aaatccatcg
     9481 cgcagcaatc gaaattcctt cctggtcagg atgtggtcgt catcggcact ggcccactcg
     9541 ggctgttctc cgtacaaatg gcgcgaatta tgggggcggt aaatatcgtc gtcgttggtc
     9601 tgcaagaaga tgtggcggtc cgcttcccgg ttgcaaaaga actgggtgcg acggcagtag
     9661 taaatggttc taccgaagat gtggtggcgc gctgccagca aatttgtggc aaagacaatc
     9721 tgggtctggt gattgaatgc tccggtgcca atatcgcact gaaacaagcc atcgaaatgc
     9781 tccgcccgaa cggggaagtg gtacgcgttg gaatgggctt caaacctctt gatttctcga
     9841 ttaatgacat taccgcctgg aacaaaagca tcattgggca tatggcctat gactccacct
     9901 catggcgtaa cgctatcagg ctattagcca gcggcgctat caaagtcaaa ccgatgatca
     9961 cgcatcgtat cggcctgtcg caatggcgcg aagggtttga tgcgatggtc gataaaaccg
    10021 caatcaaagt gatcatgact tacgactttg atgaataacc actctcctga aggaacgtta
    10081 tcgcatggaa cagataacaa aaccgcattg tggtgcacgg ctggatcgct taccggattg
    10141 ccgctggcat tcatcaatgt ttgccatcgt cgcgtttggc ttgcttgtct gctggagtaa
    10201 tgccgttggt ggcttgatcc tcgcgcagct gaaagcgttg ggctggacag ataattccac
    10261 cacagccaca ttctcagcaa tcacgaccgc cggaatgttt ctcggtgcgc tggtgggcgg
    10321 catcattggc gacaaaaccg gtcgcagaaa tgcgttcatc ctctatgagg ccattcatat
    10381 tgcctcgatg gttgtcggtg ctttctcacc gaatatggat ttccttattg cctgccgttt
    10441 tgtgatggga gttgggctgg gagctttact ggttacgctg tttgctggtt tcaccgaata
    10501 tatgcccggt agaaatcgtg gaacgtggtc aagtcgggtt tcctttattg gcaactggtc
    10561 atatccgctc tgttcattga tagcgatggg actcacgccg ctgattagtg cagagtggaa
    10621 ctggcgggta caactgctta tccctgcaat attgtcgctt atcgctacgg cgctggcctg
    10681 gcgctacttt cctgaatccc cgcgctggct ggaatcgcgc ggacggtatc aggaagccga
    10741 gaaagtcatg cggagtatag aagaaggcgt catacgccat acaggtaaac ctttgccgcc
    10801 cgtggttatt gctgatgacg gtaaagcacc acaagcggtg ccatattcag ccttactgac
    10861 aggagtatta ctgaaacgcg tgatattagg ctcttgtgtg ctgattgcca tgaacgttgt
    10921 gcagtacaca ctaattaact ggttgccaac gatattcatg acccagggga ttaatttaaa
    10981 agactcgatt gttttaaata ccatgagtat gtttggcgcg ccatttggca tttttattgc
    11041 catgctggtg atggataaaa ttccgcgtaa aacaatgggt gtggggctat taatcctgat
    11101 tgcggtgctc ggatatatct attcactgca aaccagcatg ttgctcataa cgttgattgg
    11161 tttcttcctg attactttcg tctatatgta cgtttgctat gcctcggcag tgtatgtccc
    11221 tgagatctgg ccgacagagg ccaaactccg tggctccggt ctggcgaatg cggtagggcg
    11281 aatcagtggt attgccgcac cttatgccgt tgcagtgctg ctcagtagtt atggcgttac
    11341 gggagtcttt atttttctgg gggcggtttc aattattgtc gcaattgcta tcgccaccat
    11401 tggaatcgaa accaaaggtg tctccgttga aagtttaagt attgatgcag tagccaataa
    11461 ataatacggg tggattcaaa atgaaaaatt caaaagcaat attgcaggtg ccgggcacaa
    11521 tgaaaattat ttcagcagaa ataccagtgc ctaaagaaga tgaagttttg attaaagtag
    11581 aatatgtcgg tatttgtggt tcagatgtac atggttttga atcaggtccg tttattccgc
    11641 ctaaagaccc aaatcaagaa attggcctgg gtcatgaatg cgccgggacg gttgtggctg
    11701 tgggaagccg cgtgcgcaaa tttaaaccgg gggatcgggt aaatatcgaa cctggcgttc
    11761 cttgcggtca ctgtcgttac tgtctggaag gcaaatataa catctgcccg gacgttgatt
    11821 ttatggcgac acaacccaac taccgcggcg cattaacgca ctatctgtgt catccggaga
    11881 gctttactta caaactgccc gacaatatgg acacgatgga aggggcgctg gtggagcctg
    11941 ccgcagtcgg gatgcatgcc gcgatgctgg cagatgttaa accgggtaag aagataatta
    12001 ttctgggagc gggttgtatt ggtttgatga cgttgcaagc gtgcaaatgc ctgggagcaa
    12061 cggaaattgc cgtcgttgat gtgctggaaa aacgtctgac aatggcggaa cagcttggtg
    12121 cgacagtggt tattaacggc gcaaaagaag acactattgc acgctgtcag caatttaccg
    12181 aagacatggg cgcagatatt gttttcgaaa cagcgggttc tgcggtcacc gttaaacagg
    12241 caccttatct ggtaatgcgc ggcggtaaaa ttatgattgt tggtactgta cccggcgatt
    12301 cggcaatcaa tttcctcaaa ataaatcgcg aagtcactat ccagacggta ttccgctatg
    12361 ccaatcgtta tccggttacg attgaagcta tttcttcagg tcgattcgat gtgaaatcga
    12421 tggtgacgca tatttacgat tatcgggatg tacaacaggc atttgaagag tcggttaaca
    12481 acaaacgcga cattattaaa ggcgttatta aaattagcga ttaattaata cgggagaaca
    12541 gaacatgctc gcagatatca gatattggga aaacgatgcc acaaataaac attatgcaat
    12601 tgcccatttt aacgtatgga atgcagaaat gttgatgggc gttatcgacg cagccgaaga
    12661 agcgaaatca ccggttatta tttcttttgg cacaggtttt gtcggcaaca cctcatttga
    12721 agatttctct cacatgatgg tatcaatggc acgaaaagca actgtgccgg taataactca
    12781 ttgggatcat ggtcggagta tggagattat tcataacgcc tggactcatg gtatgaattc
    12841 attaatgcgt gatgcttccg catttgattt cgaagtaaat attcgcttaa ccaaagaggc
    12901 tgtcgatttc ttccatccgc tgggtattcc ggtagaggcg gaattagggc atgtcggtaa
    12961 tgaaaccgtt tatgaggaag cgctggcggg ctatcactat accgatcctg accaggcggc
    13021 tgaatttgtt gaacgtacgg gctgtgactc attggctgtc gccatcggca accagcatgg
    13081 ggtttatacg tcagagccac aattgaactt tgaggtcgtc aaacgcgtac gcgatgccgt
    13141 gtctgttccg ctggttttgc atggcgcatc ggggatcagt gatgccgaca ttaaaactgc
    13201 aatttcgttg ggtatcgcga aaatcaacat ccatacggag ctctgtcagg ccgcaatggt
    13261 cgcagtgaaa gaaaaccagg atcagccttt cctgcattta gaacgtgaag tacgtaaagc
    13321 tgtaaaagaa cgggcattgg aaaaaattaa actgttcggt tctgatggca aagcggaata
    13381 atgatatgga taatctcgac gttatttgta taggtgccgc tattgttgat attccattgc
    13441 aaccggtcag taaaaatatc tttgatgtgg attcttatcc tcttgaaaga atcgcaatga
    13501 ccaccggggg tgatgcaata aatgaagcaa caattatttc tcgtctgggc catcgtacag
    13561 cattaatgag ccgtattggt aaagatgccg cagggcagtt tattctcgac cattgccgca
    13621 aagagaatat tgatattcaa agcctgaagc aggacgtcaa catagatacc tctattaatg
    13681 ttggactggt gacggaggat ggcgagcgga cgtttgtcac aaaccgtaac ggcagtctgt
    13741 ggaaattgaa tattgacgat gttgattttg cgcggttttc ccaggcgaaa ttattatcac
    13801 tggccagtat tttcaacagt ccactattgg atggtaaagc gctaacagaa atttttacgc
    13861 aagctaaagc ccggcagatg attatctgtg ccgatatgat caaaccgcgg ttgaatgaaa
    13921 cgctggatga tatttgcgaa gcattgagtt atgtcgatta tctgtttcct aattatgccg
    13981 aggcaaaatt actcaccggg aaagagacac tggatgaaat tgctgactgc tttcttgcgt
    14041 gcggcgtaaa aacggtggtg attaaaacgg gtaaagacgg ctgctttatc aagcgtggcg
    14101 acatgacgat gaaggtgccg gcggtcgcag gaataaccgc catcgacacc attggcgcgg
    14161 gcgataactt tgcttcaggt tttattgcgg cactgttaga aggaaaaaat ctgcgtgaat
    14221 gcgcacgctt tgccaatgca acggcggcta tctcggttct aagcgtcggt gccaccaccg
    14281 gcgtaaaaaa cagaaagctg gtcgaacaat tgctggaaga atacgaagga taatgaaggc
    14341 aaatgaaaaa gataccttta ggcacaacgg atattacgct ttcgcgaatg gggttgggga
    14401 catgggccat tggcggcggt cctgcatgga atggcgatct cgatcggcaa atatgtattg
    14461 atacgattct tgaagcccat cgttgcggca ttaacctgat tgatactgca ccaggatata
    14521 actttggcaa tagtgaagtt atcgtcggtc aggcgttaaa aaaattgccc cgtgaagagg
    14581 ttgtagtaga aaccaaatgc ggcattgtct gggaacgaaa aggaagttta ttcaacaaag
    14641 ttggcgatcg gcagttgtat aaaaaccttt ccccggaatc tatccgcgaa gaggtagaag
    14701 ccagcttgca acgtctgggt attgattaca tcgatatcta catgacgcac tggcagtcgg
    14761 tgccgccatt ttatacgccg atcgctgaaa ctgtcgcagt gcttaatgag ttaaaggctg
    14821 aagggaaaat tcgcgctata ggcgctgcta acgtcgatgc cgaccatatc cgcgagtatc
    14881 tgcaatatgg tgaactggat attattcagg cgaaatacag tatcctcgac cgggcaatgg
    14941 aaaacgaact gctgccgcta tgtcgtgata atggcattgt ggttcaggtt tattccccgc
    15001 tagagcaggg attgttgacc ggcaccatca ctcgtgatta cgttccgggc ggcgctcggg
    15061 caaataaagt ctggttccag cgtgaaaaca tgctgaaagt gattgatatg cttgaacagt
    15121 ggcagccact ttgtgctcgt taccagtgca caattcccac tctggcactg gcgtggatat
    15181 taaaacagag tgatttaatc tccattctta gtggggctac tgcaccgcaa caggtacgcg
    15241 aaaatgtcgc ggcactgaat atcaacttat cggatgcaga cgcaacattg atgagggaaa
    15301 tggcagaggc cctggagcgt taaatactca acacgattca cctctgtttc tgtgaaaact
    15361 gtgatgaaaa tctgtgattc aaacaggtta ttttgaaagt aaacatcggt tatgcgataa
    15421 tcgcgctaat gtgatgtaaa aagatacagg ggtgtaatcg tggcggcaaa agacaggatt
    15481 caggcaatta agcaaatggt tgccaacgat aaaaaggtga cagtctcaaa tttgagtggg
    15541 atttttcagg taaccgaaga aaccattcgc cgcgatcttg agaagctgga agatgaaggc
    15601 tttttgacca gaacctatgg tggtgctgtt ttaaatacag cgatgttgac ggagaatatc
    15661 catttttata agcgcgcttc atcgttttat gaagagaagc agcttattgc acgcaaggca
    15721 ctacccttta tcgacaataa aaccaccatg gcagccgatt ccagtagtac cgtaatggaa
    15781 ttgctcaaat tgttacagga ccgtagtgac ctgacgttgc taaccaactc ggcggaagcg
    15841 attcatgttc tggctcagtc agaaattaaa gtcgtttcaa cgggtgggga actaaacaaa
    15901 aatacacttt cactgcaagg aagaattact aaagagatca tcagccgcta tcatgtcgat
    15961 atcatggtaa tgagttgtaa aggtcttgat attaacagcg gcgcgctgga ctctaatgaa
    16021 gcggaagctg aaatcaaaaa gacaatgatc cgtcaggcga cagaagttgc tttattggtt
    16081 gatcactcta agtttgatcg caaagctttt gtccagttag ctgactttag tcatattaat
    16141 tacataataa ctgataaatc accgggtgca gagtggatag cattttgcaa agacaataat
    16201 atccagctgg tgtggtaatt ttagcacacg ttgtcaatat tatttgttcc tgtgcctgga
    16261 aataaaatga tgataaagat gcggcaagtg aatttgccgc aattgctacc ttttttagca
    16321 aatccgaggc acccaatgga acaatatgat caaattggcg caagactgga ccgcttgcct
    16381 ttggcccggt ttcattatcg tatatttggt attataagct ttagtctgtt attaacaggg
    16441 tttttgagtt actccggtaa tgtcgtctta gcaaagctgg taagcaatgg atggtcaaat
    16501 aatttcctca atgccgcctt tacctcggca ttaatgtttg gttatttcat cggctctctt
    16561 accggtgggt tcatcggtga ttactttggg cggcgcaggg cgtttcgcat aaatcttctc
    16621 atcgtcggta ttgctgcaac aggggccgct tttgtccctg atatgtactg gctcatcttc
    16681 tttcgcttcc tgatgggaac aggaatgggg gcgctgatta tggttggcta tgcctcattt
    16741 acggagttta tccccgcgat ggtgcgtgga aaatggtccg cgcggctctc atttgttggt
    16801 aactggtcgc ccatgctgtc tgcggcgata ggtgtggtgg ttatcgcttt ttttagttgg
    16861 cggataatgt ttatgttggg tggtattggc atactgttag cctggcttct ctcaggtaaa
    16921 tactttattg agtcgccacg atggctggca gggaaagggc aaatcgcagg tgcagaatgc
    16981 caacttcgtg aagtagagca gcaaattgaa agagagaaga gtattcgttt acccccgctt
    17041 acttcgtatc agagcaacag caaggttaaa gtaatcaagg gtactttctg gctcctgttt
    17101 aaaggtgaaa tgttacgacg tacattagtc gcgattactg ttttaattgc aatgaacatt
    17161 tcgctttata ccatcaccgt atggataccg accatatttg ttaactccgg cattgatgtc
    17221 gataaatcaa tattaatgac cgctgttatt atgattggcg ctccggtagg aatatttatt
    17281 gcggcattaa ttattgatca ttttcctcgt cggttatttg gctccacctt acttattatt
    17341 attgccgtgt taggctatat ctattcaatt cagactacag agtgggcgat tttaatctat
    17401 ggcctggtga tgatcttctt tttatacatg tatgtttgct tcgcgtcggc ggtttatatc
    17461 ccggagcttt ggccaacgca tttacgcctg cgcggttcgg gtttcgttaa tgccgtcgga
    17521 cggatcgtcg cagtcttcac gccctatggc gttgcggcat tattaacaca ttatgggtcg
    17581 atcacggtgt ttatggtact tggtgtcatg ttattgctct gtgcgctggt tctctccatt
    17641 tttggcatcg aaacgcggaa ggtgtcgttg gaagagattt ctgaggtgaa ttaaatcttt
    17701 cgttgatggc atcaattgcc agatgtgcca ctgacgtgtt ttatttggcc tacaatgcca
    17761 gtacagaatt gtaggccagc gtaaaattac ccctgcgtct cttcccagtc tgccagcgta
    17821 tatagcgttg ccccagctgc tgacatctcc ataaacgcgt gcgcactgtc ctggggctgg
    17881 atattcacgc cacgacaacc atcggtaatc acgtttacct tataacctaa ctgtaacgcg
    17941 tccagcacgg taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg
    18001 atttcatgat cgcgtaacca gtcatcgaga gaggttttct gccgacggcc gttatcaaaa
    18061 aaggcactgt aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatcgct
    18121 ttttggttca gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgatctggc
    18181 cagaaggttt gtggcaaacc gtcgagttgg cctggcgtat aaggctctac accgtgctgg
    18241 ctggcaaaac tgccgtgatt cgccgggtgc cagtcctgac tggcgataac cgcttcaccg
    18301 cgcgactggc accagtcaat caggcggtta gcgacatcca ccgtactgtc accttccggc
    18361 acggcgagcg cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga
    18421 gggggcattg cagtctcctt aatcatctgg cgtcagttcg ccgcgcaggt tttggctcat
    18481 ggccttgcga atggtttcag tatccagttc ctggctaagc aggtaatgca gtttggttag
    18541 cgtggcttct acagtcatat ctgcaccgcc aataacgcca gcatgggcga gggcgttacc
    18601 ggtggcataa ccacccatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac
    18661 aataccgcga tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt
    18721 acccacgcca taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac
    18781 gacgtcagca gaaatccctg gataaatcgt cactacgcca attggttgtg gggtgattgg
    18841 atgaacgatt aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg
    18901 gatacctgct tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc
    18961 atgggctttg gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc
    19021 gttgatcgga taattcgccg caacgtacaa cgcattcagc agattaattt gtccgtcaga
    19081 gcgtaactca gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattttc
    19141 gagcatgaac gacagcgcag aggcggtata tgccatcgta tcggtgccgt gcagaatgac
    19201 aaaaccatca tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc
    19261 cggcgtcata tctgaagaat ccatcagcgg cgtatattca tgaatggtga aatctggcat
    19321 ctccgggcga tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg
    19381 tatataaccc tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat
    19441 tgatttcttt tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga
    19501 ggcaggggga gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa
    19561 gaggattaaa aatatggccg acgatctgtc ggccactcag tacaagactt aacgcacgtt
    19621 ggcgcaggtg aggcaaaacg cataacggtt ttgtgggtcg ttaaacgcgg ccagcttgtc
    19681 actttcactt ttaacggtag aggctaccga ggccagcggt gcaggtaaca tggcctggaa
    19741 cgcatctggc aacattgccc ggacagaacc agacatgttg tccatcactt tgtcgaagaa
    19801 ggtcggttca tcaacgtagt attccagatg ccactgtttc acttttgcca gctctgctgc
    19861 tttggcaacc gcatcatcga aatccccgag actatcgacc agcccgttag cttttgcatc
    19921 ctgaccggtc cagacgtggc cctgggcgat tttatcaatc tgctccggcg tcgaatgacg
    19981 cgcatcagca accagcgtga taaagcgttt atagccattc tcaatgctta actgcatcat
    20041 ctgctgcgct tccggcggca gtgccctggt gatagaaaca tctgccagcg gtgaagttga
    20101 gacaccatca gtatgaacac caatcgaatc cagactattt tctacggtgg tgatcacgcc
    20161 gaagataccg atagaaccgg tcagggtgct ggggttagcc acaatgtaat tagctggcgt
    20221 agaaatccag taaccaccag atgccgccat gccgcccatc gatacaacca caggcttacc
    20281 cgctgcacgg gctgctgcca gttcagcgcg aatcacttca gacgcggtaa cgctgccgcc
    20341 tgggctatta acacgcagga caatcgcttt cactttcggg tcaaggcgag cgtcgcggat
    20401 ttgtgccgca gtggtatcac cgccaacatt cccctgagtt tcctcgccat ccataattgc
    20461 gccattagcg aagacgacac cgatgctgtc accggtatct gccggcgttt tcaatgcgta
    20521 atcgtaataa ctgatggcgc gataattttt atcagtctta ctccagccga actctttggt
    20581 cagtgctttt tcgatttccg cactcgatgc cagtgcatcg accagcttgt tttccagtgc
    20641 atatttcgcg gtatcgccac cggttttggt taaaccctca agcaaccctt gcgcgccagg
    20701 gaatacctgc tgagcaggga tctgtcggtt agcggcaaca gtattcagat agttttgcca
    20761 cagctcacca atccagcggc tgtcagcttc gcgggctgcc ggtgacatgt catcacgaat
    20821 aaacggttca acggcagatt tatacgtacc cacgcggaac acatgggtgg aaactttcag
    20881 cttatccagc aacgatttgt agtacagacc gttggtagca aaaccgtgca aatccaccac
    20941 gccttgcggt gacagccaaa ttttattggc gaaactggcg agataatatt gcccctggct
    21001 gtagttctcg ccgacggcat aaaccggttt cccgctgtca cgaaactctt tcagggcttt
    21061 gccgatgtac tgcatagacg gttggtcgcc gcctgcgaag tttttcagat ccatcacgat
    21121 accggtgata ttgcggtcgt ccttcgcctg gcgaatggtg ttgacgatat caaacagtga
    21181 gttttcctgc agacgatcgg aactggcacc aagtagttgg cggcttaatt tactaaaccg
    21241 ctgagaactg tcgggtttat cgacgatcac accagaaatg tccagcagca gtgcaccacg
    21301 actggccgtt tctttcgaat caccaccact gacctgcatc cagatcccca caccaaccag
    21361 tacgagaaaa ataaagaaca ggttaagtac catttcacgg acgaaattca gcagacgcca
    21421 cgtccattta aaaaatccgg caataaatcg ccaaagggtt cgcatgtatt ctcccaactt
    21481 aaggtcacac ctgtcacagg cgcaatagat gtggttatcg taatgaccca accggtactt
    21541 gtcagcagga attgcggact gtgctgtaac aaaatccact ctcgtgttaa ttttgtgaat
    21601 aaatatcacg acaggagtta atcaaatgga tgcactcgaa ctattgatca atcgccgtag
    21661 cgcctcccgc ctggctgaac ccgcgccaac gggtgaacaa ctgcaaaaca tcctgcgtgc
    21721 gggtatgcgt gcgccggacc acaagtccat gcaaccgtgg catttttttg tgattgaagg
    21781 ggaagggcgc gagcgtttca gcgccgtact ggaacagggg gcgattgctg ccggtagtga
    21841 tgacaaagct atcgacaaag cccgtaatgc gccgttccgc gcaccgctca tcatcacggt
    21901 ggtggcgaaa tgcgaagaaa atcataaagt cccgcgctgg gaacaggaaa tgtctgccgg
    21961 atgcgcggtc atggcgatgc aaatggcagc cgttgcccag gggtttggcg gcatctggcg
    22021 cagtggcgca ttaactgaaa gtccggtagt gcgtgaagca ttcggttgcc gtgagcagga
    22081 taaaattgtc ggttttctct acctcggtac gccgcagctg aaagcatcta cgtcgattaa
    22141 cgtcccggac ccgacgccgt ttgtaactta tttctgataa ttgtcgcgaa actgtctgga
    22201 ttgtgagcaa aggcgcagga attcagacac tctcacttat cacttcacgg aatgagggtt
    22261 accatacccg gattgcattg acaggagatg tccatgagcg agaactcgat tcgtttgacc
    22321 caatacagcc acggagctgg ttgcggctgt aaaatttccc caaaagtgtt ggaaaccatc
    22381 ctgcacagtg agcaggcgaa gtttgttgat ccgaatttgc ttgtgggtaa tgaaacccgc
    22441 gacgatgcgg cggtgtacga tctgggcaat ggcaccagcg ttatcagtac caccgacttc
    22501 tttatgccga tcgttgataa tcctttcgat tttggccgca ttgcggcgac taacgccatc
    22561 agcgatatct tcgcgatggg gggcaaaccg attatggcga ttgcgatcct cggctggccg
    22621 attaacaaac tttccccgga aattgcccgc gaagtaaccg aaggtggacg ctatgcatgc
    22681 cgtcaggcgg gtattgcgct ggctggcggt cactccatcg atgcgccgga gccgattttt
    22741 ggtctggcgg taacggggat cgtaccgacc gagcgggtga agaaaaacag taccgcacaa
    22801 gccggatgca aactgttcct gacgaaaccg ttggggatcg gcgttcttac cacggctgag
    22861 aaaaaatcac tgttgaaacc agaacatcag ggactggcga cggaagtgat gtgccggatg
    22921 aacatcgcag gcgcgtcctt tgccaacatc gaaggcgtaa aagcgatgac cgacgttact
    22981 ggttttggtc tgctgggtca tttgagcgaa atgtgtcagg gcgcaggtgt gcaggcgcgc
    23041 gttgactatg acgcgatccc aaaattgccg ggggttgaag agtacattaa gttgggcgca
    23101 gtgcctggcg gcaccgaacg taactttgcc agctacggtc atctgatggg tgaaatgccg
    23161 cgtgaagtgc gcgatttgct gtgcgatccg caaacttctg gcggtttgct gctggcggtc
    23221 atgccggaag cagaaaatga ggttaaagct acagccgccg agtttggcat tgaactgacg
    23281 gcgattggcg aactggtgcc agcgcgcggc ggtcgtgcca tggttgagat ccgttaattc
    23341 aatgcggttg tttattgccg aaaaaccgag tctggcgcgc gccattgctg atgtcctgcc
    23401 caaaccgcac cgtaaaggcg atggctttat cgagtgcggt aatggtcagg tggtgacctg
    23461 gtgtatcggt cacctgcttg agcaggcgca gccagacgcc tacgacagcc gctatgcgcg
    23521 ctggaatctt gcggatttgc cgattgtccc ggaaaagtgg caattacagc cccgaccctc
    23581 cgtgaccaaa caacttaacg tcatcaaacg gttcctgcat gaagccagcg aaatcgttca
    23641 cgccggggac ccggatcgcg aagggcaact gctggtggat gaagtgctgg actatctaca
    23701 actggcaccg gaaaagcgcc agcaggtgca gcgttgcttg ataaacgacc tgaacccgca
    23761 ggcggttgag cgggcgatcg accgtcttcg ttccaacagt gagtttgtgc cattgtgcgt
    23821 ttctgcgctg gcgcgagcgc gtgccgactg gctatacggc atcaacatga cccgtgcgta
    23881 taccattctc ggtcgcaatg ccggttatca gggcgtactt tccgtgggac gcgtgcagac
    23941 acccgtgctc gggctggtgg tgcgccgcga tgaagagatt gaaaacttcg tggcgaaaga
    24001 cttctttgaa gtcaaagcgc atatcgtgac acctgccgat gagcggttta ccgctatctg
    24061 gcaaccgagc gaagcgtgtg aaccgtacca ggatgaagaa gggcgcttgt tacatcgtcc
    24121 actggcggag catgtggtta accgcattag tggtcaaccg gctatagtca ccagctataa
    24181 cgataaacgg gaatcagaat ccgcgccgct gccgttttcg ctttcggcgt tgcagattga
    24241 agcggcaaaa cgctttggtc tgagcgcgca gaacgtgctt gatatctgcc agaagctgta
    24301 cgaaacgcac aagctaatca cttatccgcg ttccgattgt cgctatttgc cagaagaaca
    24361 ttttgccgga cgccacgcgg tgatgaatgc catcagcgtt catgcaccag atctgttgcc
    24421 gcagccagtg gtagatccag atatacgcaa ccgctgttgg gatgacaaaa aggtcgatgc
    24481 gcaccacgcc atcattccga ccgcacggag ttctgcgatc aacctgacgg agaacgaagc
    24541 gaaggtctat aacctgattg cccgtcagta tctgatgcag ttctgcccgg atgcggtgtt
    24601 ccgcaagtgt gttatcgaac tggacattgc caaaggcaaa tttgtcgcta aagcccgttt
    24661 tcttgctgaa gcaggctggc gcacgctgtt aggcagcaaa gagcgcgatg aagaaaacga
    24721 cggtacgcca ttgcctgtgg tggcgaaagg cgatgagttg ctgtgtgaaa aaggtgaagt
    24781 ggtagagcgg caaacccagc cgcctcgcca ttttaccgat gcaacactgc tttcggcgat
    24841 gaccgggatc gcgcgctttg tgcaggacaa agatctgaaa aagatccttc gtgcgaccga
    24901 tggtctgggg acagaagcaa cgcgtgccgg gattattgaa ctgttgttca agcgtggttt
    24961 cctcaccaaa aaagggcgct atatccactc caccgacgcc ggaaaagcgt tattccattc
    25021 gctgccagaa atggctacgc gaccggacat gaccgcgcac tgggaatcgg tgctgacgca
    25081 aatcagcgaa aagcagtgtc gctatcagga ctttatgcag ccgctggtgg ggacgctata
    25141 tcagcttatt gatcaagcca aacgtactcc ggtgcggcag tttcgcggca ttgtggctcc
    25201 gggcagtggt ggcagtgctg ataagaaaaa ggctgcaccg cgtaaacgta gtgcgaaaaa
    25261 aagtccgcca gcagatgaag ccggaagcgg ggcgatagcg taagcgagcg aatctttcgt
    25321 gctattcgag tcatattctg aaatatccag cggatcaaga aaattcgttg gatatttttt
    25381 tgcatggata aaattatcgc ctctaaagta tgtaataaca gggaatgtga tgaaaaaagt
    25441 tttattacaa aaccatcctg ggagcgagaa gtattctttt aatggctggg aaatatttaa
    25501 tagtaatttt gaacggatga ttaaagaaaa taaggccatg ctgctttgta agtggggggg
    25561 ttatttaaca tgtgttgtcg ctgtaatgtt tgtattcgca gcgataacat ccaacggttt
    25621 gaatgaaaga ggcctgatta ctgcgggatg ctcttttctt tatctattaa ttatgatggg
    25681 gcttattgtt cgggccggtt ttaaagcaaa aaaagaacaa ctgcattatt atcaggctaa
    25741 aagtattgag ccgctcagta tcgcgaagtt acaggcgcta caattgatcg caccttatcg
    25801 attctatcat aagcaatggt ctgaaacgct ggagttctgg ccgcgaaagc ctgaacctgg
    25861 caaagatacc ttccaatatc atgtgcttcc ttttgactcg atcgatatca taagtaaaag
    25921 acgcgagtct ttagaggatc aatggggtat cgaagatagc gaaagttatt gtgccttaat
    25981 ggagcatttt ctttctggcg accatggagc caataccttt aaagcaaaca tggaggaagc
    26041 cccagagcag gttatcgcct tgttgaataa atttgctgtt tttccctcag actatatctc
    26101 tgattgcgct aatcatagct ccggtaaatc ctcggcgaag ctaatatggg cggcggaatt
    26161 atcatggatg atctcgatat caagcacagc ttttcaaaac gggacaattg aagaagaact
    26221 ggcctggcat tatataatgc ttgcttctcg aaaggcgcac gagttgttcg aaagcgaaga
    26281 agattatcaa aaaaatagtc taatgggatt tctttactgg catatctgct gctatcgcag
    26341 aaagttaacg gatgctgaac ttgaagcgtg ttatcgttac gacaagcagt tttgggagca
    26401 ctacagtaag aaatgccgtt ggcccataag aaatgttccc tggggagcat catccgttaa
    26461 atactcataa acgcctgaaa ttttgccggg ggcgctgcgc ttgcccggcc tacattagag
    26521 cgtaactgca atgaattgtg cccatttgta ggcctgataa gcgtagcgcc atcaggcatt
    26581 tacaacttaa atcacaccct gcgccagcat cgcatcggca accttcacaa aaccggcaat
    26641 gttcgcgccc tgcacgtagt tggtttgctc accttcacca ccatgctcaa cacaggcatg
    26701 gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact ttctcggctt tccagcccag
    26761 gcgcgcagcg ttttgtgcca tttccaggcc tgacgttgct acaccgccag cattagccgc
    26821 tttacccggt gcaaacagta cgcctgcctg ctggaacagt tcagtcgctt cgatggtggt
    26881 cggcatattt gccccttcgg cgacggcttt aacgccattg gcgataagct gatgcgcggc
    26941 gtcaacatcc agttcgttct gggtagcgca aggcagggcg atatcaaccg gtacagacca
    27001 cggctgttgg ccttcgagat agaccagacc aaattctttg gcataatctg cgactcgacc
    27061 atcgcggctg gctttgattt cgataagacg tgccagtttc tctttcgtga atccgctttc
    27121 atcaactaca gtgccgctgg agtctgacgc agtgatcaca cgagcaccaa attccatcgc
    27181 tttttcgata gcgtactggg cgacgttgcc ggagccagaa acggaaacgc gcattccttc
    27241 aaaacccata ccgtggcgtt ttagcattgc ttctgtgaaa taaaccagac cgtagccggt
    27301 agcttccggg cgaataagac tgccgccaaa tgaaagaccc ttaccggtga atacgcaggc
    27361 ggtattgttg gagagctttt tcatcatccc cgccataaag ccgacttcac gaccaccaac
    27421 cccgatatca cctgccggaa cgtcggtatc cgcgcccagg tggcgataca gttcagtcat
    27481 cagcgcctgg cagaaacgca tcacttcgcc ttcgcttttc cctttcgggt cgaaatcgct
    27541 gccgccttta ccaccgccca tcggcagagt agtcagggca tttttgaagg tttgttcgaa
    27601 gccgaggaat ttaagaatgg agaggttaac tgacggatgg aagcgcatac cgcctttgta
    27661 cgggccgatg gcagagctga actgcacacg ccatgcacgg ttgacctgta cctgattgcg
    27721 atcatcaacc cataccacgc gaaactggat cacgcgctcc ggttcaacca gacgctccag
    27781 taatgacatc tggcgatatt ttggattttg ttcaagaaaa ggccagagtg tggtcattac
    27841 ttcacgaacg gcttgcgcga actcggtttg attcgggtcg cgcttttgga catggttgag
    27901 gaatgactcc agagaatatg tctgatccat agatataaaa cccttatata ttaatatgat
    27961 tgtgcttatg ttgtttatgt catgtgcttt tgcagttttc gactatacca tcaagaatgt
    28021 gacggtaagc aagaaaaaaa tgacccagga aagcaaatta ataacgagag taatctcata
    28081 accaaatgcg ataactcaga cgctaaagtt ttgccacccg gctaccgtta tcagtagcag
    28141 agacaataac gttaaggagt tccctgtgag tcgagcattg ttcgccgtag tattagcatt
    28201 tccattaatt gcactggcta acccgcatta tcgtccggac gtggaggtaa acgtaccgcc
    28261 agaagtgttc agttcaggcg ggcagtcagc gcagccctgt actcagtgct gcgtttatca
    28321 ggataaaaat tattctgaag gggcggtaat taaagccgaa ggaatactgc tgcaatgcca
    28381 gcgggatgac aaaaccctta gcactaatcc gctggtctgg cggcgcgtaa agccataaac
    28441 gcctctaata atggaatgtc agcaggggcc agcggatatt gcagcgcctc ttcaggtgag
    28501 caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc cgtggaagtc gggtacgtgc
    28561 caggcatgaa gatggataat ccgccccgaa acttctcgct gatggctggc aacatactca
    28621 cccacagttg cttcgatgcc caattcttcg tttaactcac gcaccagcgc ctgctgctgg
    28681 ctttcatcca gctcgacttt accaccggca aactcccata atcccgcctg atcgctctgg
    28741 gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa tgatggcggc aacaacttca
    28801 atcattttca taaattacac acgggcagat aagcgaaaaa tgccataata tccgcactgt
    28861 tgctcaatca gaaaggcttt atatttaagg agtgcctgtg ctagaccgcc atcttcaccc
    28921 ccggattaaa ccgttgttgc atcagtgcgt gcgggctctt gataaaccgg gcattacgcc
    28981 agatggttta acattagtgg gatttgccat cggcgtgctg gctctgccgt ttctggctct
    29041 gggctggtat ctggcggcgc tagtcgtcat tttgttgaat cgtctgcttg atggtctgga
    29101 tggcgcactg gcgcggcgca gagggcttac cgacgcgggc ggctttctcg atatttctct
    29161 cgattttctc ttttacgcgc tggtgccgtt tggttttatt cttgctgcac cggagcaaaa
    29221 tgcgctggca ggcggctggc tgctgtttgc gtttatcggt acgggcagca gttttctcgc
    29281 ttttgctgcg ctggcggcga aacatcagat tgataatccc ggttatgcgc ataaatcgtt
    29341 ttattatctg gggggattaa ccgaaggcac agaaacgatc ttactgtttg tgctgggatg
    29401 tttattccct gcatggtttg cgtggtttgc gtggatcttc ggtgcgttgt gctggatgac
    29461 gacctttacg cgggtgtgga gtgggtatct gacgctgaag tcgctccagc gtcagtaatt
    29521 gcaatgttat ttgctgctgt ccgggccgcg ttcaccggtt gctaccggat tttttggatc
    29581 gctgctccat tcgtaccagc cgccgtcgta aacggagacg ttattccagc ccattgctcg
    29641 tgcgtacata aaggtttcgg acgcgcgcca gccggtgccg cagtagaatg aaacttgctg
    29701 atctggtttg atattccatg ctttccacat agcggtaata tcatcggcgc tacgcatggt
    29761 gccatccgga ttatggaaat cttccatatg cgtcgagtcg ctaccagcgt gtccccaacg
    29821 tgctccggct atttcacctt ttggtttaat atagctgtaa ccgctggtcg taccgataaa
    29881 ttctggccac gaacgaatgc tcaccagcga tgcatcctgg cgatgcagca gtccacgggc
    29941 ctgttccata tcgagcatca actgcggttg tgccgggatc ttcacgccga aatccggttc
    30001 cgctttcact ttcggtggcg taccgcgctc aacgggcaga cccgcgtcgg accaggtttg
    30061 ccagccgcca tccagcaggc gcacatcttt cacgccggca taaagcataa tctgcgccac
    30121 acgcgctgca gcgtatacgt cacgcccgta cagaatgacc gtggtgtcat ggcgaatgcc
    30181 gtgttttgcc agcatcgctt ttagttgttc atcagaaact ttgttccaca gcggttcgct
    30241 ttccacttcg ttggtatcga tgtagtcagc accgggaata tggctgataa ggtaaagctt
    30301 aggagcgccc caggccgctt caatgacttt ccagtcaccg gcaggtttcg ccgtaacctc
    30361 tttaccttgt tgcaggtcgt gcagccattg cggataaacc agctgctcaa agtgcggcag
    30421 tttttgtaga cgggaaggtt cgcttagcgc gtcactcaga atggagatat gcgtaaaacc
    30481 tgctttttgc agtcgcgttt tgacggcgtc gacatctttg ccattaccgt acagcgccac
    30541 cggggcatcg gctttcaggt tatgttgctt gatccacgcg ttgagctgtt cggtgctcat
    30601 tttgtcaagc cagctagcag agaggtttaa ggcggcaggt tcatgaccag aagggccatt
    30661 taaggtttgt ggccagccgt tataaaacgc gctggggcga gtatctatcg ctttgccatt
    30721 ttgttgttga agctggtcaa gtgtaagagg cttcgccagt tcagcggccc acgaagaagc
    30781 gcaagccagc cctaaagcca ttgccagcgc ggtcatttga gaaacacgtt tcatcgaaaa
    30841 tcccgacatt gtagttttga gtgcgccatt ctgaactgac gcattgagaa aaactttgcg
    30901 ttatcgcagt ttgttgtaat tttctgacca ctgcgccata tccagaacag aactatcagc
    30961 aggaacatcc tggagatcgt gcgttacctg aacgacgggg atcgccagtt cgcgaacttc
    31021 gctgaacacc cactggcgaa aattatcgcg cagagccaca tcaagacggc tgaatggctc
    31081 atccagtagc aacgcttttg gctgagcgag aagggcgcga agcagagcaa cgcgcgctcg
    31141 ctgaccgcca gacaaagtgg caggatcctg atggaaagct ccgtcaaggc ctgaacgctc
    31201 aagtgcatca ttcacggcat tacgtcgggc attcccctta agtgtcgccg gtagcgccag
    31261 cagtaaattt tgcccgacac tgaactggtc aaataacagt gcatcctgaa aaagaatgcc
    31321 aatctgacgc tgtgcggtgg gtagcatgtc aatccgttgc tcattgagcc atagctcacc
    31381 tgtacaagaa aactgtccag ccagtgcacc aatcatccat gaaaacagag tggattttcc
    31441 acagccagac ggccccatta acgtgacaat gtcaccttta tccaccgtaa agttaacgtt
    31501 tgtcagcaag caactttctg gtaaacgtag cgaaacattt ttcacgcaga gcattagcgg
    31561 agtccttgtc tgacataacc tacccatttt gcgactaacg cggtcagggc aaaaataata
    31621 agcggtaata gcagttgcca taaagcctgg gcggcgagaa taccgttgct gccgccgctg
    31681 cttaatgcca ccgcctcagt ggtgagcgtc ggaaaacgcc ccgcgcccag ccacagtgtc
    31741 ggcatatact gcgcaatact gactgaaaat cccaccgcga aggcaatcag cgtagggcgt
    31801 agaataagcg gacatttcac gtaaaagaag attttggccc gcgaccagcc cagtgtttgc
    31861 gcaatcaaaa ttaaccgtga atcaatgcgc tgccaggcgg gttgcaggat aaacagtatc
    31921 cacggcatta cccacagcag atgcccccag accaccgccg tccagctccc atccaggttc
    31981 agccagagcg ccagcgtgta ctggcccgcc accagtggca gagcgggtaa taaaatgggc
    32041 aaccatagcc agaagtgccg acgtgacgat ccacattcca gccacgccag tatcaggatt
    32101 aaagcgataa acgtcgcggt aagtcccatt gtcaggctgt tgatgagcgc ttcactattg
    32161 atcgtcgact gatccgcgag gatcgccagc agaaccacac acagcacacc ggtcaagggt
    32221 aaaaaactcg ccagggtaat gcctggcaat aaaggcgtgg caggcttacg aacgccatct
    32281 acgcggggaa tagtacgccg ccaggtacac catagcagat agcccagcaa aacgtaggcg
    32341 gcgagtaaca acatcagcag caggctggcg agtgcgcctt tcgtttgttg atcggagtcg
    32401 ccctgagtta accactgcca gctaattact gccagcgtcg gtggattacc tggcccaaga
    32461 ataatcgcca catctacgac cgacagcgac caggcaacaa tcgccagcat cgccattgcc
    32521 agcgcaggcg cgacggaggg caacagcagc cagttcaggc attgccagcg gctgtagccc
    32581 agcgaatcca gcacgatgac ttgctgtaaa agccgttttt cactcaatac ggctgctaag
    32641 atccacagca gaaatgcgct ttctttcacc gccagcgtga agcccagccc aatacccaat
    32701 ttgtccattg gcggagtgaa atacgggaaa tagtcataaa gcagccctcc gtcagcaaag
    32761 agcagaaggg cgctggtggc aaaagccaca tggggaatgg cgagcagcca cggcagacgg
    32821 gtgcacatac gctgccattt cggcccaggc cacagcgcca caatcaccag cagggcaatc
    32881 aacaacgccc cgaccgccgc gatggttgtc gacaccagcg ttgccagtaa tgcctgcggt
    32941 aactgtggat cggcaaataa cgcctgccag tgtgtcaacg acaaagcagg cgatatcagc
    33001 gtgagagctg ccgggatcag cggtgcataa atcaccgcca ccatcgccca cagcaaaaag
    33061 attaatgcgt accgtaacgg tgtagccatt cttgttccag cgcatttacc caacctgcgt
    33121 gcggttcagc cagtaccggc ggcagatctt gcggcattct tgattgcaat gtttcgcgct
    33181 gcccgtcagg cagtttttgc ggatcgagaa tggaaggatc tccccagaca gcagggtctg
    33241 ctttacgcag ttgcgcatcg ggtgagagca ggaagttggc aacgactttt gccgcagcac
    33301 tggcattcgc gttggcagga atggtgacga aatgcacgtt gccaatcatc ccctcgcgaa
    33361 agccaaaact gtaactgctt gcaggcaaat cgccgctggc gattttttgc tgcgcatgcg
    33421 cggggttaaa ggtcagcgac aggcgcaatg tgccggcttt cagcagagca tccatccgcg
    33481 cgggcgaagg cgggaaatcc tttccttcgc gccacaaata cggatgcagc gcatcaagat
    33541 attgccacaa gggagcagtg acacgggcga aagttgcatc gtccggcgct tcttttaatg
    33601 cggcgggatc gggcgtcagc ataatcagca actgttcaag aaacgccgtg ccggtaaagt
    33661 ccggtgggcg cggataggta accgtgccgg gattggcttt agcaaactcc agtaaggctt
    33721 gcggcgtttg tggtggctgt ggcgtaacat cgcggcgggc gataaatgtc agttgtgcgc
    33781 ccccccaggg cgattcagcc ccttctgtag gcactgaaaa atcttcccgc accggcagct
    33841 gtgtgtcgac atagcgccag ttgggcagag tctctgccca gtcggtttgc agtaaattgg
    33901 cctcttttaa ggtgcggaag ttttcgccgt tcacccagag cagatccacc gagccgcccg
    33961 ttttacgtcc ggctgcggct tcggtctgaa tgcgcttcac cgcgtctgcg gcatccgcca
    34021 gacgaacaat cttcaggttt atagcgtaat gggttttcat ctcgccgcta acccagtcga
    34081 gatagcggtt aattgcggta tcgccgcccc aggcgttaaa ccagacagtt tgccctttgg
    34141 cctcattttt aatttcttgc cagtcactgg catgtgttgc cagagaaaac agcgataaca
    34201 atcccagcaa ccacccacaa tggcgcatca tctctcctta agaacgtttt tccggaatac
    34261 ggcacagccc ctgagcggga atgtccatgg cagaattaaa acgggctgac agattttgca
    34321 agccgatcaa tgccgtcagt tcggtgagcg cctgggcgtc aaaatgcgta gtcagtcggg
    34381 tgcgcagggc atcatcgacc gttggcggtg tgacgcttgc ggcttcggcg tactccagcg
    34441 ccagccgttc ttcatcgcta aagagcgggc tttgtcgcca gtcagctacc gccagcaatt
    34501 tatcggtgct gccggtgcgc tcggcgactt tcatactggt gatatccacg caaaactcac
    34561 acagacacat ttgcgcgata cgcgcgctga caagcgatcg caccactgga tcgagcggtg
    34621 agcgtttgcg ctccagccag ccaacaaaca tcgacaccag ataaaagata aacgggatcc
    34681 gcccccacca gcgaatcggg cttagcacct cgccgtaatg gcgacgatgc agccacgcct
    34741 gcggacgtaa aatgaaagga attttgctaa gcggcggtaa acccatcagg cattcctctt
    34801 ctggcgctca agccatattt tcttcgccat aaaaatcacc acggtcaggg caaacagaat
    34861 aaacagcccg gtgacaaacc agaatgtgcc gcccgttaac atgctgcctg cccaggaata
    34921 gacaatggtt gcgggtaact ggccgatccc ggtggcgatg ataaattgcc gaaaacgaat
    34981 tgaagtcaga ccggcagcat agcttacggg atcaaaaggc acaaaaggca ataaccggca
    35041 taccagaatg gtgtgtttac catagcgggt aaaaaagccg tccatactat tgagtaccgt
    35101 tttacccgtt aacttttcca ccacttcgcg gcccagcact ctggcgataa aaaagcatag
    35161 cgccgcgccc gccatcgcgc tggtccacga tagtagtccg ccccagaacg caccaaacag
    35221 cgaggcattg gcaaaggtga tcaaaaacgc aggtagcggt gcagcaatgg cctgtaaaat
    35281 catcaacaag aacgagacaa ctgccgccag tgcgccgtaa gactgaataa agcgttctat
    35341 accctgttgg tccacggcgg caaacgccgc aacgctgcga ttgataaact catggacgcc
    35401 gggaagcagc gcccatgcca gcagcatagc gaacaacagg atgatgaggg ttatacggta
    35461 gtaccagatt ttacgcgatt gcatcttcat catcctttat cctggcttta ttcggtgatt
    35521 ttaaaggtaa ccgttgccag cgtgttatcc gccgggagaa ccgaggcatt gcctttgaat
    35581 ttaacttcac ctcgtttttc aaccgcacca taagtgtatg ttgcattgct gacgatgccg
    35641 accgggcagc tatccagaca caccaggcaa cccgttttct tctcttctgc tgccgttaaa
    35701 ttaccgccaa agcgcatgtc cagtttcttg ccattactgt caacaatcac ttcatcgaag
    35761 gaatacgttt ttgccgcccc ttgccagttg accgaaatat ccagtttgct gcccgtgaca
    35821 tgagtcgttt ctttattatc catcgtcatg ttttcgcccg gcgtaccacc tgcctctttc
    35881 agggcttcat aaaatgcttt cggggtcgca taacccataa acagcgattt atgtccgttg
    35941 gagccatctt taaacacaat accgtgacga gtgtcgtcgg tgaggaaacg accattaatt
    36001 tgcaccagca tggttaccgt atggttattg gcatcgacgc gcagcggttg gctgttcgaa
    36061 agtccgtcgt attccacttt tgccgccgta ttctcttttt gatcgcagcc gctaagccca
    36121 gcaacagcca gtaagcagag tgcagtcagt ccttttttcc atgacactga ataatgttgc
    36181 aacatcgttc tatccttcat tttttgggtg agtaagtggg ttgcggcgcg aagcagacag
    36241 atccacatgt ttgtgtcggg cgtagagttt ggcgagctgg acgagaataa acagcgccag
    36301 gcccgccaga cagagttgta aaataaagcg cagcgtaatg ccttcattgg cgagatcgct
    36361 tgccatcacg gtataaatta cgattccggg cagggtggtt aatgccgaaa taagggtata
    36421 gggccagaaa gcaatagcgg ttaatccgta agcgtaattt tgaatattgt acggaaacag
    36481 cggaattaag cgggtcagaa taagaaaatc gatgccgtta cgcgcaatgc ctttttctat
    36541 agccttgaag gtatggctat ggcccacgta tttcagcagt aaatcacgtc ccatccagcg
    36601 cgccagcagg aatgagcacg aggaggccag cgtggcggca attaatgaga gtagtgtccc
    36661 taagaacggg ccaaaaacta ttcctcccgc gatcaccaac atgcttcctg gtaacagaaa
    36721 gagggtggca atgatgaata acagaatata gaggctataa ccgaacaatc cgctctggcg
    36781 aatgagtgtc tgtaagtggg gtaaatcggt gagcagatcg aataaaccga aagcgtggat
    36841 agcgtaaatg accagcgcaa aaataagaca ggcaaaaaga aatttacgct cagcgttcat
    36901 cgtggagttt cctatatcaa acttgataat agagaagtgg tcagacaact aaaaacgcag
    36961 tgacggtata acaaaggacg gcaggcaaca aatcattact gacagtttgt tgatttttct
    37021 caaggttaat tctcctgacc cagtttgagc caggagagct gctaagttag cggcggaagg
    37081 tcgcccagac gggggcgtga tcggacggtt tttccatgct gcggatttca tagtcgatgc
    37141 cggtttctac gcaacattct gccagcggtt ggctggcgag cagcaggtcg atgcgcagac
    37201 cacggttatc gtcaaaacct tttgagcggt aatcaaacca tgagaaacga tctgctgttt
    37261 gcggattcgc atggcggaag gtatcaacca atccccagct catcagtctc tccatccatt
    37321 cgcgttcctc cggaaggaaa gaacattttc cggtacgcag ccagcgctta cggttctctt
    37381 cgccaatgcc aatatccagg tcgccaggac tgatattcat atcgcccata atcagcaccg
    37441 gattttcacg cttgagttcg gtttccagat agttttgcag attctgataa aactgcgcct
    37501 ttgccgggaa ttttatcgga tggtcgcggc tttcaccctg cgggaagtaa ccgttgatca
    37561 cggtgacatt acccagcggt gaggggattt ccgccataat aatccgccgc tgcgcctctt
    37621 cgtcgtcacc gggaaagccg cgacgcacgg caatcggcgt ctctttggtc agcagcgcca
    37681 cgccataatg gcctttctgc ccgtgataaa acacgttgta gccgagcttc gccacctctt
    37741 cgagcggaaa catatcgtca tgaacttttg tctcctgcag gccaatcaca tccggttggt
    37801 gcttttcgac gatggcttca agctggtgag gtctggcgcg caggccgttg atattaaaag
    37861 agacaaattt catagtcgct gccatttatt cagataaaga gtgcgtggat ggtagcagaa
    37921 tttcgctttg cttaccgcct gttgttaagt gatttgatcg atggttgtta atgatgtcaa
    37981 cgatggcgca aaaaatgccc gcttttggtg cgagctgcgt cagaatggcg cagtaatttc
    38041 cagtaaattt cgtcaatgat cacaattcca gaattatatt ttacttctgc ataacattgc
    38101 gttttttatt ctttttattc acccacatcg caaacgtatt cacttaatat gcactttaaa
    38161 tgcatatggt ttggttataa ctccttgatt tccagttagc ctccgcggtt tatgcacttt
    38221 tatcactggc tggcacgaac cctgcaatct acatttacag cgcaaacatt acttattatt
    38281 aacatataaa taacgaatta tttactgtag aggtcgctat gtctcagcca attacgcgtg
    38341 aaaactttga tgaatggatg atacctgttt acgctccggc accgtttata ccggtacgtg
    38401 gcgaaggttc gcgcttgtgg gatcagcagg ggaaagagta tatcgacttc gcgggtggta
    38461 ttgcggtgaa cgcgctgggc catgcgcatc cggaactgcg tgaggcgctg aacgaacagg
    38521 cgagtaagtt ctggcatacc ggcaacggtt acaccaacga gccggtactg cgactggcga
    38581 aaaaattgat cgacgccacg tttgccgatc gcgttttctt ttgtaactcc ggtgcggaag
    38641 ccaacgaagc ggcgctaaaa ctggcgcgta aattcgctca cgaccgctac ggcagccata
    38701 agagcggtat cgtggcgttt aaaaatgcgt ttcatggtcg cacgctgttt actgttagtg
    38761 cgggtgggca gccagcctat tcacaggatt ttgcgccact gccaccagat atccgtcatg
    38821 ctgcatataa cgatattaac tctgccagcg cgctgattga cgactctacc tgtgcggtga
    38881 ttgtcgaacc catccagggg gaaggcggtg tggtgccagc cagcaacgca tttttacaag
    38941 gtctgcgtga attgtgtgat cgccacaatg cgctgttgat ttttgatgaa gtacaaaccg
    39001 gcgtcgggcg caccggggaa ctgtatgcct atatgcacta cggcgtgacg cctgatctgt
    39061 taactaccgc caaagcgctg ggcggcggtt tcccggtcgg tgcgttgtta gcaaccgaag
    39121 agtgcgccag cgtgatgacc gttggcactc atggcaccac ctatggcggc aatccgctgg
    39181 cctcggcggt ggcaggcaaa gtgctggagc tcatcaatac gccagagatg cttaacgggg
    39241 ttaagcaacg tcacgactgg tttgttgagc gtcttaatat tattaatcac cgctatggtt
    39301 tgttcagtga agttcgcggc ttaggtttgc tgattggctg tgtactgaat gccgattacg
    39361 ccggacaagc gaaacagatc tctcaggagg cggcgaaagc gggcgtgatg gtgctgattg
    39421 ctggcggtaa tgtggtgcgt tttgcgcctg cgctcaatgt cagcgaagaa gaggtgacga
    39481 ccggactgga tcgctttgct gcggcctgtg aacgctttgt gagcggaggt tcatcatgat
    39541 ggtcatccgt cccgttgaac gctcagatgt ctcggcgctg atgcagcttg ccagcaaaac
    39601 gggcggcggc ctgacgtcgc ttcccgccaa tgaagccacg ctttcggcgc gtatcgaaag
    39661 ggcaatcaaa acctggcaag gcgaactgcc caaaagtgag cagggctatg tgttcgtgct
    39721 ggaagatagc gagacaggca ccgtggcggg gatttgtgcc attgaggtgg cagttgggct
    39781 gaacgatccc tggtacaact atcgcgtcgg cacgctggtt cacgcttcga aagagctgaa
    39841 tgtctataac gcgctgccga cgctgtttct cagtaacgat cacaccggca gcagcgagct
    39901 gtgcacgctg tttctcgacc cggactggcg caaagagggc aacggctatt tgctgtcgaa
    39961 atcgcgcttt atgtttatgg cggcttttcg cgacaagttt aatgacaaag tggtcgccga
    40021 aatgcgcggg gtgattgatg aacacggcta ttcaccgttc tggcaaagcc tcggcaagcg
    40081 cttcttttcg atggatttta gccgcgccga ttttctctgt ggcaccgggc aaaaggcatt
    40141 tattgcagaa ctgatgccga aacatccgat ctatacccac tttttatccc aggaagccca
    40201 ggacgtcatc ggtcaggtac atccgcaaac cacgcctgcc cgcgcggtgc tggagaaaga
    40261 aggttttcgc taccgtaact atatcgacat cttcgacggt gggccgacgc ttgagtgtga
    40321 catcgaccgc gtgcgcgcca tccgtaaaag tcggctggtg gaagtggcag aagggcagcc
    40381 tgcgcagggc gattttccgg cctgcctggt cgccaatgaa aattatcacc atttccgcgt
    40441 ggtgctggtg cgtaccgatc cggcaaccga gcgtttgata ttaaccgccg cacaactgga
    40501 tgccctgaaa tgccacgccg gggatcgcgt tcgtctggtg cgcctgtgcg cagaggagaa
    40561 aacagcatga ctttatggat taacggtgac tggataacgg gccagggcgc atcgcgtgtg
    40621 aagcgtaatc cggtatcggg cgaggtgtta tggcaaggca atgatgccga tgccgctctg
    40681 gtcgggcagg cttgtcgggc agcccgtgcg gcgtttccgc gctgggcgcg gctctcattt
    40741 gctgaacgtc aggcagttgt cgaacgcttt gccgcactgc tggaaagcaa taaagccgaa
    40801 ttaaccgcga ttattgcccg agaaacgggt aagccgcgct gggaagcggc aaccgaagtg
    40861 acggcgatga tcaataaaat cgcgatatca attaaggcgt atcacgttcg tacctgcgag
    40921 cagcgtagtg aaatgccgga cggcgcggcg agcctgcgac atcgcccgca cggcgtgctg
    40981 gcggtgtttg ggccgtataa tttccctggt catttgccga acggacatat cgttccggca
    41041 ttgctggcag gtaacaccat tatctttaaa cccagcgaac tgacaccgtg gagtggcgaa
    41101 gcggtaatgc gtttatggca gcaggctggc ttgccgccgg gcgtgctgaa cctggtgcag
    41161 ggcgggcgtg aaacgggtca ggcgctgagt gcgctggagg atctcgacgg tttgctgttt
    41221 accggcagcg ccaataccgg ctaccagttg catcgccagc tctctggtca gccggagaaa
    41281 attcttgccc ttgagatggg tggtaataat ccgctaatta tcgatgaggt ggcggatatc
    41341 gatgccgctg tccatctgac cattcagtcg gcgtttgtca cagctggtca acgctgcacc
    41401 tgcgctcgcc gtttattgtt gaaaagtgga gcgcagggcg atgcgtttct tgctcgtctg
    41461 gttgccgtta gccagcggtt aacgccgggc aactgggatg acgaaccgca gccgtttatt
    41521 ggcgggctga tttctgaaca ggccgcacag caggtggtta ctgcctggca gcaattggaa
    41581 gcgatgggcg gacgaaccct gcttgcgccg cacttgctgc aagccggaac atcgttgctg
    41641 acgccgggga tcattgaaat gacgtgcgtt gctggcgtgc cggatgaaga agtgtttggg
    41701 ccactcttgc gcgtctggcg ttatgacaat ttcgatgagg cgatcaggat ggcgaataac
    41761 actcgcttcg gcctctcttg cggtctggtt tcccccgagc gggaaaagtt cgaacaactg
    41821 ttgctggaag cgcgggcggg gattgttaac tggaacaaac cgcttactgg tgctgccagt
    41881 accgcgccat tcggcggcat tggtgcatcc ggtaaccatc gccccagcgc ctggtatgcc
    41941 gcagattact gcgcatggcc tatggcgagc ctggagtcgg actcgttaac tttgcccgca
    42001 acgcttaacc ccgggctgga tttttccgat gaggtggtgc gatgaacgcc tgggaagtta
    42061 atttcgacgg gctggtaggg ctgacgcaca attacgctgg cctgtcgttt ggtaatgaag
    42121 cctctacccg tcaccgtttt caggtgtcta acccgcgact ggcggcgaaa cagggcttac
    42181 tgaaaatgaa aacccttgcc gatgcgggat tcccccaggc cgtgatcccg ccgcacgagc
    42241 gtccgtttat tccggtgctg cgtcagttgg gattcagtgg tagcgatgag caggtactgg
    42301 aaaaagttgc acgccaggca ccgcactggc tttccagcgt cagctccgct tcgccaatgt
    42361 gggtagccaa tgcggcaacg atcgcgccat ctgccgatac gctggatggc aaagtgcatc
    42421 tcaccgttgc caacctgaac aataaatttc accgttcgct ggaagcgccc gtcactgaat
    42481 cgctgttaaa agctattttt aacgacgaag agaaatttag cgtccattcg gcgttgccgc
    42541 aggtagcgtt gctcggtgat gagggggcgg caaaccacaa tcgtctcggc ggtcattacg
    42601 gtgaaccggg tatgcaactt tttgtctacg ggcgagaaga aggcaatgat acccggcctt
    42661 cccgctatcc ggcgcggcag actcgcgaag ccagcgaggc ggtggcaagg ctgaatcagg
    42721 tgaatcccca acaggtgatt ttcgcccagc aaaacccgga cgttatcgac cagggcgttt
    42781 ttcataatga cgtgattgcc gtgagtaacc gccaggtgct gttttgtcat caacaggcgt
    42841 tcgctcgaca ggcgcagtta ctggcaaacc tgcgtgctcg ggtcaatagc tttatggcaa
    42901 tagaggtgcc cacggcgcag gtttccgtgt cggatgcggt gtctacatat ctgtttaaca
    42961 gccaactgct gagccgcgat gatggttcca tgatgttggt gctgccgcag gagtgtcggg
    43021 aacacgccgg agtatggggt tatctcaatg aactccttgc cgctgacaac ccgattagcg
    43081 aactaaaagt ctttgattta cgtgaaagca tggcaaacgg cggcggtccg gcgtgcctgc
    43141 ggttgcgcgt ggtattgaca gaagaagaac gccgggcggt gaatccggca gtgatgatga
    43201 acgatacgct gtttaatgcg ctcaatgact gggtggatcg ttactaccgc gatcgtctta
    43261 ctgctgccga tctggccgac ccgcaattgc tgcgcgaagg gcgggaagca ctggatgtat
    43321 tgagccaatt actgaatctc ggttcggttt atccgttcca gcgcgaggga gggggcaatg
    43381 gataattttc ttgctctgac cttaacgggt aaaaaaccgg ttatcaccga gcgagaaatc
    43441 aacggcgttc gctggcgctg gctgggcgat ggtgtgctgg aactgacgcc attaacgcca
    43501 ccgcaaggcg cactggtgat ttcagcggga atacacggta atgagacggc acctgtggag
    43561 atgctggacg cgttgcttgg cgcgatatct cacggcgaga tcccgttacg ttggcggttg
    43621 ctggtgatcc tcgggaatcc tcctgcgctg aagcaaggga aacgttattg ccatagcgat
    43681 atgaaccgaa tgtttggcgg tcgttggcag ctgtttgctg aaagcggaga aacctgtcgg
    43741 gcgcgcgaac tggaacagtg cctggaagat ttttatgacc agggtaaaga atctgtgcgc
    43801 tggcaccttg atctacatac cgcaattcgt ggctccttgc atccgcagtt cggtgtatta
    43861 ccgcaacgcg acataccctg ggacgagaaa ttcctgacgt ggctgggtgc ggcgggtctg
    43921 gaggcactgg tgttccatca ggagcctggt ggtacgttta cccatttcag cgccagacat
    43981 tttggcgcgc tggcctgtac gctggaactc ggcaaagcgt tgccctttgg acaaaacgat
    44041 cttcgccagt ttgcagtaac tgccagcgca attgctgcgc tgctatctgg tgagagtgtc
    44101 ggtatcgtga gaacaccgcc gctccgttat cgggtggttt cgcaaattac tcgccactcg
    44161 ccgtccttcg aaatgcatat ggcaagtgac acgctgaatt ttatgccgtt tgagaaagga
    44221 acattgctgg cgcaggacgg ggaggaacgt tttaccgtaa cccatgatgt agagtatgtg
    44281 ttattcccta atcctttggt agcgttggga ttacgcgcgg gattaatgct cgaaaaaata
    44341 agctaatact tacccgcaga aatcattctg
//
LOCUS       DAPGRN010000027        43636 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPGRN010000027 DAPGRN010000000
VERSION     DAPGRN010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.027"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            137..886
                     /locus_tag="RHW33_001617"
     CDS             137..886
                     /locus_tag="RHW33_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1645137.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            1155..1373
                     /gene="osmB"
                     /locus_tag="RHW33_001618"
     CDS             1155..1373
                     /gene="osmB"
                     /locus_tag="RHW33_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV1645138.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(1499..1825)
                     /gene="yciH"
                     /locus_tag="RHW33_001619"
     CDS             complement(1499..1825)
                     /gene="yciH"
                     /locus_tag="RHW33_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV1645139.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(1825..2562)
                     /gene="pyrF"
                     /locus_tag="RHW33_001620"
     CDS             complement(1825..2562)
                     /gene="pyrF"
                     /locus_tag="RHW33_001620"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV1645140.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLITPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(2755..3924)
                     /gene="lapB"
                     /locus_tag="RHW33_001621"
     CDS             complement(2755..3924)
                     /gene="lapB"
                     /locus_tag="RHW33_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV1645141.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(3931..4239)
                     /gene="lapA"
                     /locus_tag="RHW33_001622"
     CDS             complement(3931..4239)
                     /gene="lapA"
                     /locus_tag="RHW33_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV1645142.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(4388..5152)
                     /gene="pgpB"
                     /locus_tag="RHW33_001623"
     CDS             complement(4388..5152)
                     /gene="pgpB"
                     /locus_tag="RHW33_001623"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV1645143.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5322..5912
                     /gene="ribA"
                     /locus_tag="RHW33_001624"
     CDS             5322..5912
                     /gene="ribA"
                     /locus_tag="RHW33_001624"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV1645144.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(5976..8651)
                     /gene="acnA"
                     /locus_tag="RHW33_001625"
     CDS             complement(5976..8651)
                     /gene="acnA"
                     /locus_tag="RHW33_001625"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV1645145.1"
                     /translation="MSSTLREASKATLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VARSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVLCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(8815..8910)
                     /locus_tag="RHW33_001626"
     CDS             complement(8815..8910)
                     /locus_tag="RHW33_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645146.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(9024..9191)
                     /locus_tag="RHW33_001627"
     CDS             complement(9024..9191)
                     /locus_tag="RHW33_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645147.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(9194..9358)
                     /locus_tag="RHW33_001628"
     CDS             complement(9194..9358)
                     /locus_tag="RHW33_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV1645148.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(9653..10627)
                     /gene="cysB"
                     /locus_tag="RHW33_001629"
     CDS             complement(9653..10627)
                     /gene="cysB"
                     /locus_tag="RHW33_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV1645149.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(10837..13434)
                     /gene="topA"
                     /locus_tag="RHW33_001630"
     CDS             complement(10837..13434)
                     /gene="topA"
                     /locus_tag="RHW33_001630"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV1645150.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            13814..14065
                     /locus_tag="RHW33_001631"
     CDS             13814..14065
                     /locus_tag="RHW33_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV1645151.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(14101..15150)
                     /gene="sohB"
                     /locus_tag="RHW33_001632"
     CDS             complement(14101..15150)
                     /gene="sohB"
                     /locus_tag="RHW33_001632"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV1645152.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            15370..16128
                     /locus_tag="RHW33_001633"
     CDS             15370..16128
                     /locus_tag="RHW33_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV1645153.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCRSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            16125..16715
                     /gene="cobO"
                     /locus_tag="RHW33_001634"
     CDS             16125..16715
                     /gene="cobO"
                     /locus_tag="RHW33_001634"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV1645154.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADPSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(16755..17627)
                     /gene="rluB"
                     /locus_tag="RHW33_001635"
     CDS             complement(16755..17627)
                     /gene="rluB"
                     /locus_tag="RHW33_001635"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV1645155.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17840..19735)
                     /locus_tag="RHW33_001636"
     CDS             complement(17840..19735)
                     /locus_tag="RHW33_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HDV1645156.1"
                     /translation="MAGIYRCILLLIVAFFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPDGVLDIEENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYRIISVTRDGIPEP
                     WHLNWHIGLMSIVVGDKHSFLPQGDYDYQIHYQVKNAFLREGDSDLLIWNVTGNNWPF
                     EIYKTRFSLKFPDIAGNPFSEIALFTGEEGDTYRNGRILEDGRIESSDPFYREDFTVL
                     YRWPHSLLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILIRV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSSF
                     YRPDTFLQWGGVAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLPFLFCSLELLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQTGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALPGWAVSAN
                     LGREWVARITPSLTAAVSAPEIARSGVLFSLQMHLSLGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(19763..20383)
                     /locus_tag="RHW33_001637"
     CDS             complement(19763..20383)
                     /locus_tag="RHW33_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV1645157.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(20380..21261)
                     /gene="yciV"
                     /locus_tag="RHW33_001638"
     CDS             complement(20380..21261)
                     /gene="yciV"
                     /locus_tag="RHW33_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV1645158.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHLLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRAHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HDGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            21399..21443
                     /gene="trpL"
                     /locus_tag="RHW33_001639"
     CDS             21399..21443
                     /gene="trpL"
                     /locus_tag="RHW33_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV1645159.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    21420..21513
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            21535..23097
                     /gene="trpE"
                     /locus_tag="RHW33_001640"
     CDS             21535..23097
                     /gene="trpE"
                     /locus_tag="RHW33_001640"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV1645160.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQSLSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSLLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            23097..24692
                     /gene="trpD"
                     /locus_tag="RHW33_001641"
     CDS             23097..24692
                     /gene="trpD"
                     /locus_tag="RHW33_001641"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV1645161.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            24693..26054
                     /gene="trpCF"
                     /locus_tag="RHW33_001642"
     CDS             24693..26054
                     /gene="trpCF"
                     /locus_tag="RHW33_001642"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV1645162.1"
                     /translation="MMQTVLAKIVADKAIWVEARKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIASGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVLDKAKVLSLAAVQL
                     HGNEDQQYIDTLRGALPANIAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            26066..27259
                     /gene="trpB"
                     /locus_tag="RHW33_001643"
     CDS             26066..27259
                     /gene="trpB"
                     /locus_tag="RHW33_001643"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV1645163.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTKTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDEEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            27259..28065
                     /gene="trpA"
                     /locus_tag="RHW33_001644"
     CDS             27259..28065
                     /gene="trpA"
                     /locus_tag="RHW33_001644"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV1645164.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            28446..28625
                     /locus_tag="RHW33_001645"
     CDS             28446..28625
                     /locus_tag="RHW33_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV1645165.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQNGGNKSGKS"
     gene            28711..29211
                     /locus_tag="RHW33_001646"
     CDS             28711..29211
                     /locus_tag="RHW33_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV1645166.1"
                     /translation="MNMKTMEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKESLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            29257..29763
                     /locus_tag="RHW33_001647"
     CDS             29257..29763
                     /locus_tag="RHW33_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV1645167.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(29823..30461)
                     /gene="ompW"
                     /locus_tag="RHW33_001648"
     CDS             complement(29823..30461)
                     /gene="ompW"
                     /locus_tag="RHW33_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV1645168.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            30701..30790
                     /locus_tag="RHW33_001649"
                     /pseudo
     CDS             30701..30790
                     /locus_tag="RHW33_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            30818..31561
                     /locus_tag="RHW33_001650"
     CDS             30818..31561
                     /locus_tag="RHW33_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV1645169.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITMVLGHVFSPS
                     DAQLAQLNDGVPVGGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            31591..32130
                     /locus_tag="RHW33_001651"
     CDS             31591..32130
                     /locus_tag="RHW33_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV1645170.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            32235..32633
                     /gene="yciA"
                     /locus_tag="RHW33_001652"
     CDS             32235..32633
                     /gene="yciA"
                     /locus_tag="RHW33_001652"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV1645171.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(32673..33392)
                     /gene="tonB"
                     /locus_tag="RHW33_001653"
     CDS             complement(32673..33392)
                     /gene="tonB"
                     /locus_tag="RHW33_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV1645172.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            33616..33912
                     /locus_tag="RHW33_001654"
     CDS             33616..33912
                     /locus_tag="RHW33_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV1645173.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            34212..35465
                     /gene="kch"
                     /locus_tag="RHW33_001655"
     CDS             34212..35465
                     /gene="kch"
                     /locus_tag="RHW33_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV1645174.1"
                     /translation="MSHWATFKQTATTLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(35520..35693)
                     /locus_tag="RHW33_001656"
     CDS             complement(35520..35693)
                     /locus_tag="RHW33_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV1645175.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            35836..37296
                     /gene="cls"
                     /locus_tag="RHW33_001657"
     CDS             35836..37296
                     /gene="cls"
                     /locus_tag="RHW33_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV1645176.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            37331..37660
                     /locus_tag="RHW33_001658"
     CDS             37331..37660
                     /locus_tag="RHW33_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV1645177.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(37713..38717)
                     /gene="oppF"
                     /locus_tag="RHW33_001659"
     CDS             complement(37713..38717)
                     /gene="oppF"
                     /locus_tag="RHW33_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV1645178.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTHPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(38714..39727)
                     /gene="oppD"
                     /locus_tag="RHW33_001660"
     CDS             complement(38714..39727)
                     /gene="oppD"
                     /locus_tag="RHW33_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV1645179.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(39739..40647)
                     /gene="oppC"
                     /locus_tag="RHW33_001661"
     CDS             complement(39739..40647)
                     /gene="oppC"
                     /locus_tag="RHW33_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV1645180.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(40662..41582)
                     /gene="oppB"
                     /locus_tag="RHW33_001662"
     CDS             complement(40662..41582)
                     /gene="oppB"
                     /locus_tag="RHW33_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV1645181.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(41668..43299)
                     /gene="oppA"
                     /locus_tag="RHW33_001663"
     CDS             complement(41668..43299)
                     /gene="oppA"
                     /locus_tag="RHW33_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV1645182.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT        10978 a        11171 c        10465 g        11022 t
ORIGIN      
        1 gttgctgaat ctacgccaga catcccgaag gcaccgtggg agatttaaca atcgtgcctt
       61 caggttttcc tatttgagga tgcccggcgc gtcagtattt tttattaagt attataacgt
      121 tataagaatt acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca
      181 gggtcaggtt agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg
      241 ccaggatctc aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt
      301 ttcgcttgat agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga
      361 actcgccgag tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg
      421 cagcagcaat gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat
      481 cacggtcagc agctacatcg cgcatttgct gaaagacgcg ccttgtgaag ttattttgct
      541 cggtggcgtg taccagaaaa aaagcgaaag tatggttggc ccgttgacac gccagtgcat
      601 ccaacaggta catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg
      661 attcaccggt cgcgatatga tgcgtaccga tgtggtgaat gccgtgctgg aaaaagagtg
      721 cgaagcgata gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg
      781 tcccgttgag cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat
      841 gcatcttgag cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc
      901 tacggatgat ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta
      961 agactattta cctgttatta acactctcaa gatataaaat tattatcagc gatataacag
     1021 gaagtcatta tcacctgcgt gatataaccc tgcgcgcgag cggatttcac ggaataattt
     1081 caccaggctt attcttagct attatagtta tagagagctt acttccgtga atcataaatt
     1141 caggagagag tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta
     1201 ccctggcaat gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa
     1261 tcggcgcggg tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta
     1321 cattaggtgg cgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa
     1381 taaatgtcga ttatcgactc gcaattataa tacgcttgat tctgttcagt acgaaataac
     1441 tgaataatac acacagtaaa atattcgaaa gccacggata tatccgtggc tttttatgtt
     1501 aaccgcctgc gagttttacc ttcatccctt tcgcttcaag tagtgatttt aataaatcac
     1561 gcttatcgcc ctggatttca ataactccat ctttaactgc tccgccgcag ccgcattttt
     1621 tcttcagttc cgctgcaagt tttgtcagtt cggcatcatc gagatcgata ccggtaatca
     1681 ggcaaacgcc cttaccttta cgtccactgg tctgacgctg aatacgcacc acaccatcgc
     1741 ctttagggcg tactggggcc gctttgggct catcaatacg cccggtttct gttgagtaga
     1801 ccagacggct gttggaatca ctcatcatgc actccgctgt aaagaggcgt tgatcgcttt
     1861 cagcgtctgc gctggatcta ccgattgcgt taccgggcga ccaatcacca tataatcaac
     1921 accagccgcc aacgcctgtt ctggcgtcat aatgcggcgc tggtcacctg cttcactccc
     1981 ctgcggacga atgcccggtg taatcagttt gaactcctga ccgaatacct gtttaaagcg
     2041 cacagcttcc tgagcagaac acaccacacc atcaaggcca catttttgcg tcagtgccgc
     2101 cagacgttct gcataatctg caggtgacag tgtcacgcca agatcggcca ggtcgctggc
     2161 ttccatgctg gtcaacactg tcacagcaat caaaagcggt gcatctttgc caaacggaac
     2221 cagtgcctca cgcgctgcgg tcatcatacg cgccccacca gaggcatgaa cattcaccat
     2281 ccacacgcct aagtcagctg cagcagcgac agcgtgcgct gcagtgttgg ggatatcgtg
     2341 gaatttcagg tcaagaaaga tatcaaaacc acgctgttga agttcgcgca caaactgtgg
     2401 cccaaacaat gtaaacatct ctttgccgac cttcagacga caatcgcgtg ggtcgatctt
     2461 gtcgacaaag gccagcgcgg catcacgatt atgataatca agggcaacaa ccacaggaga
     2521 attcgtaaca gcgcgggaag aagatgaagc agttaacgtc atgaccagac cttcttgatg
     2581 atgggcaccg gaagtggcgc gaaacaggta aacggcgagc attctacccg tggaaataaa
     2641 aaattaacag ttgcgatttc tcccctggca ggctttttct gcgccgtcgt tgccggagca
     2701 caatgggaca tcaataacta gtatgttgta actaaagtcg gtttttttaa aaaattacag
     2761 gccatcaaga ccgcgaatcg gtttaatagt tgaccaggca cgacaagacg gacaatgcca
     2821 gtagagggtg tatgcggtaa aaccacattt ctggcagcga taacgaggct tactacgtac
     2881 cttctcgcca accatgtcac gcagcaccat caggctctct ttggcacgcc cttcttccgc
     2941 ttcatttaag tggtaatcca ttaacttatg gaacacacgc atggtcggat gacgctgaag
     3001 ctggcgcgta atatagacct gtgcagcctc actaccgtcg cgcgcttcga tgatatccgc
     3061 aagcatcaat tcagcatcgg caccggtgtt ctcttccacc gcgcgctgca ggaattctgc
     3121 ccattcggca gttttaccca actgctgata gcaggtttgc aacatttcca gcgtttcgct
     3181 gaccagttct ctgtcctggg atatcacgcg ttgcagactt tcgacggctt tggcgtattc
     3241 tcctttcgcc ataaacacgc gtcccatcat gatggatacg cgggcgctgt ttttatctgc
     3301 cgccgcccct ttttttagca aggtcatggc acgatcgaga tcgtcgctgg ccatatgctg
     3361 cagggctaac tcacagtaga aatgggcaat ttcgacgcgc tgtttatctt tacccagctt
     3421 caccaggcgt tcggcaacat caattgcttt ctgccactca ctggtagcct ggtagatttg
     3481 tagcaactgt tgcagcgcgc caatgcggaa gtcagtttca tcggtcagct gattgaacat
     3541 gtcttccgcg cggtcatata acccggcggc catgtaatca cgccccagtt gttgaatcgc
     3601 caacagacgc tgttcatagg tcagcgaggc gctttccatt agggtctgat ggatgcgaat
     3661 agcgcgatca acttcgccac gcgaacggaa caggtttccg agcgtaaggt gggcttcaac
     3721 ggtgcctgta tcctctttaa gcatatcgag aaacaggtct accgctttat cctgttgatt
     3781 actaagcagg aagttaaccc ccgctacgta atcacgcgac aagcggttgg cttcatcttg
     3841 cttgttttgt tgcgcacttc tgcggcccat ataccagcca taggcagcgg ctacaggcaa
     3901 aagcagaaac aacaactcca gcatagaaag ttattccttc gccgctgacg agtgcggcac
     3961 tacagccacg tcagtcgcgg gtgaaagctg gttttccagt cgctttattt tacgttcagc
     4021 gcgcgccagg gaaacacgaa ctcgcagcca gaacaggcca caaatcaacc aaccgatagc
     4081 aaaccccgca gcaaacaata ccgccagcaa tgtggaaata cggtactccc cttgcgctaa
     4141 cagataatta aacgtcacct gttgatcgtt ctgcgcaccc aacgtgaccg aaatcacgaa
     4201 gatcgctaac accagtaaga aaatgagtaa atatttcaca ttacttcccg ttatgtggtt
     4261 agagcgaata aattatggtc aacttaccgc aatagccctt taaagtacca ttttcgcgcc
     4321 gtctgcgaaa tggcatccac gcgcattgtt gatgatttaa gggctaaatt cggaaaatca
     4381 accagcgtta actttcttgt tctcgttgcg ctatttcgcg attttcttcc gcaggtggtg
     4441 ttaatggccc acaaattcgt tgcgcaagcc acgttgccac cgccaccagc gcccacgaaa
     4501 tcaacgtagc tactaccaga tcgcgtggcc aatgcatccc gagcagcagg cggcttccca
     4561 tgactccagt tgcccagacc agcaagatag caatggttaa cgttcgccga cgcggccaca
     4621 gcaaaccaac ggccagcagt gcccaactgg cagcaaacat cgtgtgaccg gaaggaaagg
     4681 caaaccccgt ctctttctgc cagtgtgaac gcaaatattg tgggatattt ttctcttcag
     4741 ccaactgttc tttcactaga tttccgcgtt ctgctcgctt taaagtgtag aactcatcaa
     4801 ccggaatatg atgtgttttt tccagccaga taacaaaagg tcgtggttcc tggactttgt
     4861 ctttgatcca ggatttaacg ccttgtccca caaggattgc ggccgccaga atggcaaata
     4921 atacaaaggc agccttaatg cgaaaacgca gacaccagag aaaccagccg aataaaatca
     4981 aatgtgtaat gacgccccag ggctgggtga cagtttcagt aacccaaaaa gccgctttta
     5041 gtagccaact ttgttctcca ggttgccaac gccagccaga aatccatacg gctactggca
     5101 taacaagcaa tagtgcagct cccactgcgg tacgtcgggc aatcgaacgc atggcctctc
     5161 ctttttgata agtcccacaa tcataactga aaatgccagt tccaggaaaa attgacagat
     5221 ttgtgccatt ccgtgaacga tcgacgcgtc gtgattaggt gaaccccttc tcgttatggc
     5281 aaaataagcc aatacagaac cagcattatc tggagaattt catgcagctt aaacgtgtgg
     5341 cagaagccaa actgccaacc ccatggggcg atttcctgat ggtgggattt gaagaactgg
     5401 caaccggaca cgatcatgtc gcgctagtct atggcgatat ttccgggcat accccggtac
     5461 ttgcgcgcgt ccattccgaa tgtctgaccg gtgacgccct gttcagcttg cgctgcgatt
     5521 gtggcttcca gctcgaagcg gcattgacgc aaattgccga ggaaggccgt ggtattttgc
     5581 tgtatcaccg tcaggaaggt cgtaacattg gtctgctgaa taaaatccgc gcttacgcac
     5641 tgcaggatca aggttacgat actgtagagg ctaaccacca gttaggcttc gccgctgatg
     5701 agcgcgactt cactctttgc gctgatatgt tcaaactcct tggcgtcaat gaagtccgct
     5761 tattaaccaa taacccgaaa aaagtagaaa ttctgaccga agcagggatt aatattgttg
     5821 aacgcgtacc attgattgta ggtcgtaacc ccaataatga acattatctc gataccaaag
     5881 ccgagaaaat gggccatttg ctgaacaaat aaccctcttg cattgtgtaa ttcatttgct
     5941 tgccggaagc aaaataaccg gcaagcaaat acttgttact tcaacatatt acgaatgaca
     6001 taatgcaaaa tgccgtcgtt ctggtagtag gtcaactccg tcgcggtgtc gatacgacaa
     6061 cggcagagta cgacttcctg gctaccatcc gcgcgcgtaa gcgtcaccgg aaccgtcgcg
     6121 ccgggttgta gattttgcag atcgccaata tcaatcttct cttccccggt tagccctaac
     6181 gttttacgcg ttacgccttg cggaaattcc agcggcagga tgcccatgcc aattaaattc
     6241 gaacggtgaa ttcgttcaaa cgactcggca atcaccacac gaattccaag cagacgagga
     6301 ccttttgccg cccagtcacg gctggagcct gatccatact ctttcccggc aatcaccgcc
     6361 agcggcgttt gctcctgctt atagcgcatc gcggcatcat agatagagac aacatcgctg
     6421 tcaggtaaat gccgcgtcat ccccccttca acgccaggca ccatttcatt acggatgcga
     6481 atattggcga aggtgccgcg catcatcact tcatggttac cacgccgcga accgtaggag
     6541 ttaaagtctt tccgctcaac accccgacct tgtaggtatc gacccgctgg gctgtcgggc
     6601 ttaatactgc ccgccggaga gatatggtca gtggtgactg aatcccccag cattgcgagg
     6661 atccgcgcac cgtgaatatc ttccacaggt gctggtgttg cctgcatttc atcaaagaaa
     6721 ggcgataaac gaatataggt tgaatcctcc tgccatccgt aggtatcgga tcgtgcgacg
     6781 ttaatttctt tccactctgc agtgccttca aagacttctg cgtactcttt gcggaacatt
     6841 tctgtggaga cttgttctac cgcacgggca atttcttgtg ccgatggcca gatatctttc
     6901 agataaaccg gatcgccttt acgatcatga ccaataggct cagaagccag gttgatattc
     6961 atatttcccg ccagcgcata ggcaaccacc agcggcggcg aggccagcca gttagtttta
     7021 accagcggat ggatacggcc ttcaaagtta cggttgccgg acagcactgc accgacggtt
     7081 aaatcgcctt ttttgattgc cgtttcgata ggatcgggca gcggcccaga gttaccaata
     7141 caggtggtac aaccgtatcc cacaaggtta aaccccagtt cgtcgagata cggtgtcagt
     7201 ttcgcttttg ccagataatc agaaacgact ttcgaacctg gtgccagcga cgctttgacc
     7261 cacggttgcc gcttgaggcc cagagttacg gcttttttcg ccagcaagcc tgcggccatc
     7321 agcacacttg ggttagaggt gttggtgcac gaggttatcg cagcaatgac cacagcgcca
     7381 tcaggtaact gatactgatg tccgttcata acataatcga ccggctggcg atctttatgc
     7441 gtggcattca cttccagttc gttactggcg gcaaatgctt ttggtacatc gggcagtgca
     7501 acgcgatcct gtgggcgttt tggccctgcc aggctcgctt caacgtcatt catatccagt
     7561 tctaacgtac tggtaaaaat cggttcatcg cccgggttac gccacatgcc ctgcgctttg
     7621 gcatattttt cgaccaactc gacctgatct tcgctgcgcc cgcttaaacg catgtaatcg
     7681 agggttacag catcgattgg gaagaagcca caggtggcac catattctgg cgacatattg
     7741 gcaatggtgg cgcgatccgc caacggtagt gaatccagac catcaccata aaattcgacg
     7801 aatttcccga ccacgccatg tttgcgcagc atttgggtaa cagtgagaac caggtctgtg
     7861 gcggtaatac cttcacgtaa ttttccggta agtttgaagc ccactacgtc cgggataagc
     7921 atggaaaccg gctggcctaa cattgcggct tctgcttcga tcccaccaac gccccacccc
     7981 agcacgccaa ggccgttgat catggtggtg tgcgagtcag taccaacgag tgtatccgga
     8041 taagcaatcc attcaccgtc ctgcaattca ctccacactg ctttgccgag atattcgagg
     8101 ttaacctgat ggcaaatgcc tgtgcctggc ggcacgacgc taaaccgact gaacgcctgc
     8161 tttccccatt tcaggaaaac ataacgttcg tggttgcgct ccatttccag gcgtacgttt
     8221 tcttcaaatg cctcatcatc accaaaacga tcgacggtca ccgagtggtc aatgaccagg
     8281 tcgaccggtg agagcgggtt aacctttgca gtatcgccgc cgaggcgttt aaccgcttcg
     8341 cgcattgccg ccagatcaac aacggcaggt acgccggtaa agtcctgcat cagcaccctt
     8401 gccgggcggt aggcaatttc acggtcagca tgggcatttt tcagccatcc tgccagcgcg
     8461 tggatatcct cttcggtaac cgagttacca tcctgccagc gcagcaggtt ttcgagcaaa
     8521 actttgagtg acttgggtag acgggtgata tcgcccagtg atttagcagc aagcggcagg
     8581 ctgtagtagt ggtaagtttt atctttggcc tgcaacgtgg ccttactggc ttctcgtagg
     8641 gttgacgaca tagctcctcc ttaatgacag ggttgcgaag ccctgattct cttcagggtg
     8701 aatattaaag ataacacaaa catcgcgtaa cgatttgata acaacccaaa ttgataaaag
     8761 aggagaaagc ttgtggcaaa gcaaatgaca accccgccgc agcggggtca aggatcagtg
     8821 agtaatgatc cagaggagtt cggcccagaa gagcatcgac agagaaaacg cgcccatcca
     8881 ggaccaatat ttcatatttg tgttgatcat aggctacacc cttaacggtt attattttgt
     8941 gttgagataa ctcaagctgt tattgctgcg gtgtattaaa agccctttaa ttcattaaaa
     9001 atgggtaata agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct
     9061 ataaaggctt gctgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc
     9121 cgtttcgttt ctgtttcatt gtcactatgc tggcataatg gtgacgactc cagttgctcc
     9181 tgaccgacca tagttatccc cacactttcc aaatcagcag tgcgacaacc acccagaaaa
     9241 gtgcagaacc aagaaaaacc gccagccagg ctttacgttt taattcagga tcgcggcggg
     9301 gttcctgatt tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga
     9361 cgttgaacga ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca
     9421 aaaatccagc gaatttacga atcgtctcca gtgaataatt caatcttttg acctgaaata
     9481 gatatttgaa aactggagtt tgcgaggcgg gtaattagac actactttac aggtaattta
     9541 ccctataaat atgagggata aaaatattgt gcacggtata gaaataagta acaaaagaga
     9601 atatattccg gcaccttcgc tacataaaag gtgccgaaaa taacgcaaga aattattttt
     9661 ccggcagttt tatatcttta aacatgacct caatttcttc attagagcgc aatgcgacag
     9721 ccgcatcaac gacatcacgc gttaaatgcg gtgcaaaacg ctgaatgaaa tcatacatat
     9781 aactgcgcaa gaaagtacta cggcgaaaac caattttggt tgtactgtgg ctgaagatat
     9841 cgtgagcatc aacacgcaca aggtcgggat cggcgaccgg atccaccgcc atgctggcaa
     9901 tgacccctac ccccagccct aaccggacgt aagttttaat gacgtcagca tccgttgccg
     9961 tgaagacgat acgcggcgtt aaccctgcgc ggttaaaggc agtatccagt tctgaacgtc
    10021 cggtaaagcc gaaggtatat gtcaccaacg gatattgcgc cagttcttca atggtaatgg
    10081 cttttttgcc tgccagcggg tgatccggag tgactacaat agcccgattc cagtggtagc
    10141 acggtaacat cactaaatct tcatacagat gcagcgcttc tgtggcgata gcgaaatcag
    10201 cattgccttt agagacggca tcagcaattt gtgtcggcga gccctggtgc atatgcaaag
    10261 aaacgcgggg ataacgctca ataaagcctt tgataacgtt tggtaacgcg tagcgtgcct
    10321 gggtatgcgt ggtggcgata tacagcgagc ctttatccgg ccaggtgtgc tcaccggcaa
    10381 ccgattttat ggcatcgact ttcgacagga cttcgcgagc gatacgaatt atttcttgtc
    10441 ctgctggcgt tacctgcgtc aggtgcttgc cgcttcggga aaaaatttga atgcctagct
    10501 cgtcttccag cattctgact tgtttactga tcccgggttg tgatgtgtaa agtccttccg
    10561 ctgttgatga gacattcagg ttatgattga ccacctcaac aatatagcga agttgttgta
    10621 atttcatgtt aaaccatcca cttagacgcc atcagaacat cgttttttcg tcacacctgg
    10681 cgggtgacta atacgattta ataataaaaa aggtgctaac tataaccact atatcattta
    10741 tagccttagt gtataggttt gaagaaaaaa taatatgcaa taaaaaaggg ccgctttcgc
    10801 gaccctttgt ttataaaaac ctgacagaat taaaggttat ttttttcctt caacccattt
    10861 gccatcaaca taaaatgctg accagccagt cgcctttccg tctttttccg aagagacata
    10921 ttgctgtttg gttttacggc taaagcgaac catggtctta ttaccttccg gatcctgctg
    10981 tggcgcatcg gccagataac gcagtttttc cggcagacgg tcgcgaaagc gataaagctc
    11041 ttccaccagt ggcgcacgcg tttcacgcga tttcgggaaa gtgttggcag ccaggaacac
    11101 accggcagca ccgtcacgca gcacgaaata ggcatctgat ttttcgcacg gcagctccgg
    11161 taatggtacc ggatcttctt tcggtggagc cacttcgccg ttacgtaaaa tcttacgcgt
    11221 gtttttacac tcttcgttgg tgcaggccat atatttaccg aaacgcccca ttttcaggtg
    11281 catttcagaa ccacattttt cacactcaac gatcgggccg tcataacctt taatgcggaa
    11341 ttcgccctct tcgatctcgt aaccgtcgca ggttgggtta ttaccacaga catgcaactt
    11401 acgtttcgga tcgatgagat agctgtccat cgccgtgccg catttcgggc aacgacgttt
    11461 tgcgcgcagc gcgttggttt cagcgtcttc gccttccagc acgttcagca cttcgttttc
    11521 cggcaccagg ttaatggttg ttttgcaacg ctctttcggc ggcagcgcat agccagaaca
    11581 gccaaggaat accccggtgc tcgctgtgcg aatacccatt ttgcgaccac aggtcgggca
    11641 gtcgatgctg gtcagaacca tctggttcgg acgcataccc ccctcttccg gatctttttc
    11701 agctttatct aactgctgag tgaaatccga gaagaagtga tccagtacag ctttccactc
    11761 tgcttcgtga tttgccacct ggtcgaggct gttttccatc tgcgcggtga agtcgtagtt
    11821 cattaactcg cggaaattct cttccaggcg gtcggtgacg atttcgccca ttttttccgc
    11881 atagaaacga cggttttcta cacgcacata gccacgatcc tgaatggtcg aaatgatcga
    11941 cgcataggta gacggacgac cgataccgcg tttttcaagt tctttaacca gcgatgcttc
    12001 actgaaacgg gctggcggtt tggtaaagtg ctgggctggt gtcagttcaa cgagcgtcag
    12061 agcatcgcct ttatcgactg ccggtaagat acgatcttca tcgcctttac gcagtgcagg
    12121 catcactttt gtccagccat caaagcgcaa aatacgaccg cgtgctttca ggcggaaatc
    12181 gcccgcacca acggtcagcg tcgtggagtc atatttcgct ggggtcatct ggcaagcaac
    12241 gaactgacgc cagatcaact ggtacagttt ctgcgcatct gcttccatat ccttcaacga
    12301 ttccgccatc acattgacgt cagaaggacg aatcgcttcg tgcgcttcct gtgagttttc
    12361 tttgctggcg tactgattcg gactttccgg cagatatttc ttaccaaaat tatcgctgat
    12421 ataaccgcga accatattta ccgcgtcctg actcaggtta gtagagtcgg tacgcatgta
    12481 agtgatatag cctgcttcat acaaacgctg cgccatcatc atggtttttt tcacgccaaa
    12541 tccaagacgg gtgctggcag cttgttgcag cgtagaggta ataaaaggag cgccaggttt
    12601 actggttgtc ggtttgtctt cacgttccag cacgctgtag cgcgcttttt ccagcagact
    12661 taccgcagcc tgagtttgtt ctttgttgac cggacggaac ggtttgtcgt tctgatgagt
    12721 cacctgcaac gccaacgctt caccagatgg cgtggtcgtg ctggcatcga cttcccagaa
    12781 ctcttccggc acgaacgctt taatttcacg ctcacgctcg accaccagac gaaccgccac
    12841 cgactgcaca cgaccggcag acagaccacg agcgatcttt ttccatagca gcggcgaaac
    12901 catatacccc accacgcggt ccataaagcg acgcgcctgc tgggcattaa cacgatcaat
    12961 attcagctca cccggtttgt taaatgcctg gcggatcgcg tttttagtaa tttcgttaaa
    13021 caccactcgg ctatagcgcg catcatcccc cccaataact tcccgcaggt gccatgcaat
    13081 ggcttcccct tcgcggtcaa ggtcggttgc gagatagatg tggtcggctt tttcagccag
    13141 ttgtttcagt tcagagacga ccttctcttt accaggcaac acttcatagt gcgcctccca
    13201 attgtgccac gggtcaaccc ccatacggtt gacgagagcg ccacgttcat cctttttagg
    13261 ctttttagcc gtcttggtgg aggtagagtc ggcactcttt ttggcagctg agccactggt
    13321 cggcaaatcg cggatgtgac cgacgctgga tttcaccacg tagtcactac ccagatactt
    13381 gttgatcgtt ttggcttttg ccggggactc aacgatgaca agagctttac ccatattcac
    13441 cttacctaat ttgatccttc caggaatgcg tcgcacgttg ataaaccttc cactggcaac
    13501 gagttaccga tatggactcg ctctctgcgg atatcaaccc cttgtcgtga ctctgttcac
    13561 gaaaattcag gtgactgagt taacaggtct tggcctacag gcgctatagc acgacggaat
    13621 tttggtcgaa tgtcaagcaa atctattgcc agattgacga aagcgtcaca ctgtacctga
    13681 taaaatccga tacgcaactt tattagcatg caaaatccaa agttgccagt aaggcgctct
    13741 tgccaccagc gtggtatatt tgtctgcaag ccatgcctgg cgtagactaa tgtccttgtt
    13801 taaggagtaa ataatgaaca aagagacaca acccatcgac cgcgaaacgc tgttgaaaga
    13861 ggccaacaaa atcattcgtg agcatgagga tacgctcgca gggattgaag ccaccggcgt
    13921 cacgcagcgt aatggcgtgc tggtatttac cggcgactac tttttagatg aacaaggatt
    13981 gccaacagcc aaaagcaccg cggtgtttaa tatgttcaaa cacctggccc atgtgctttc
    14041 agaaaaatat cacctggttg attaaagcaa aacgcgaggt cttaccctcg cgtttgtctt
    14101 ttacatcaat ggcttttgcc cccgctgcca ccagcgtaac aacaatcgat cggcgctctc
    14161 tgccgcgctg ccggtgaatc ggtcaatgag tcgtttacgc tgcatatagc gtacattgac
    14221 cacttctcgg ccttccatca ggctaagaat aacttcatca ctggtgttga tttcatcaac
    14281 caggcctttc tctaccgcct gttgtccgta ccagtgttca cccgttgcca cctgttcaat
    14341 atccagagac ggacgcatac gcttcacaaa atctttaaat aactgatgcg tttcgttcag
    14401 ctcttcgcgg aatttctccc gcccttcttc ggtattttca cccagcaagg tcagcgtacg
    14461 cttatactgc ccggcggtgt gaagttcgat atcaatatct ttgcttttca ggaagcggtt
    14521 aaagttgggc atttgcgcca ccaccccaat ggaacccaca atagcaaacg gtgcggaaac
    14581 aattttgtcc gccacacagg ccatcatgta accgccgctg gcagcgactt tgtctaccgt
    14641 aacagttaaa gggatgtttt tatcacgcag acgctgcagc tgcgaagccg ccaacccgta
    14701 accatgcacc atgccaccag ggctttccag acgtagtaca acctgatcct gcggtttgaa
    14761 tgctgcgagt acagccgtta tctcttcacg tagcgagttc acttcatggg cgtccatgct
    14821 gcctttaaaa tccagcaccc agacgcgggg tttactgtca gttgccacct ctcccagttt
    14881 ggctttcgct tttgctgctt tcgcttcttg cttatgcttt ttcttctgcg ctttgtgcca
    14941 ctgtttttgc tgatgtgagt ccatcagcgc cgcggccagt tcttctttca tctccttata
    15001 ctgttcgctg agattgttga cccgtaactc gccacgctgg cgtttattac gttgagcaac
    15061 attgacaata atggcggcaa tcgccgcaat cgctagcaca acggtaacga ttttcgccaa
    15121 aaacaaacca tattcagaca acaattccac gagtccccac cttggttaaa caacgctttg
    15181 gctcacagtt tacaacaggc atgttaagcc gtctcgcaga gatgttattg cagcgaaagt
    15241 atgacaaagt atccattttg cggtgttatc gccccaggct gattgaaatg cccttctgtt
    15301 tcaggcataa agccccaaag tcataaagta cactggcagc gcgacagaca acgtcgttga
    15361 ggagtagcca tgcattacca gccaaaacaa gatttactca atgatcgcat tatcctggtg
    15421 acgggagcca gtgatggtat tggtcgtgaa gccgcgatga cgtatgcacg ctatggtgcg
    15481 acagtgattc tgttgggccg taatgaagaa aaattacgtc aggtagccag ccacataaac
    15541 gaagaaactg ggcgtcagcc acagtggttt attctcgatt tgctgacctg caggtccgaa
    15601 gattgccaac aactggcaca gcgcattgcc gttaattatc cgcgtctgga tggtgttttg
    15661 cataatgccg gattgctcgg cgatgtttgt ccaatgagcg aacaaaatcc acaggtctgg
    15721 caggacgtca tacaggtcaa cgttaatgcc acctttatgc tcacccaggc actgcttcct
    15781 ttattactca aatcggacgc cggttcactg gtctttactt catcaagcgt tggacgtcag
    15841 ggacgagcca actggggcgc atatgcagcg tcgaaatttg ccaccgaagg gatgatgcag
    15901 gtactggccg atgaatatca gcagcgcctg cgcgtcaact gcattaaccc aggcggtacg
    15961 cgcaccgcaa tgcgtgccag cgccttcccg accgaagatc cacagaaact taaaacaccc
    16021 gctgatatca tgccgctcta cctctggctg atgggcgatg acagccgccg taaaaccggc
    16081 atgacctttg acgcccaacc gggccgtaaa ccaggaattt cccaatgagt gatgaacgct
    16141 accaacagcg tcagcagcga gtgaaagaaa aagtagatgc tcgtgtggcc caggcccagg
    16201 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
    16261 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
    16321 aaggcacctg gcctaatggc gaacgcaatc tgctggagcc acatggcgtt gagtttcagg
    16381 tgatggcaac gggctttacc tgggatacac aaaatcgcga gtctgatacc gctgcctgcc
    16441 gtgaagtctg gcaacatgca aagcggatgc ttgccgatcc ttcactggat atggttttgc
    16501 ttgatgagct gacttatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
    16561 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
    16621 atattcttga actggcggat acggtgagtg agctgcgccc catcaaacat gcgtttgatg
    16681 ccggtgtaaa agcgcagata gggatcgatt attaatctgt aaaaatgccg gatatgacgt
    16741 ccggcattct ttgattaacc gttattacgc ccgccagaac gacgaccgcc gctcacctga
    16801 ctgtgacgtt tcaccgctcg acgaatctga ttcgccttca tgcgacgacg gtctttttct
    16861 acagcgactt tagagctggt ttctggcggt agctccacca gttcgcgcag atagttagtc
    16921 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
    16981 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
    17041 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
    17101 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccaactgcac gccacgactc
    17161 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcata ttcacgttca
    17221 acttcacggc ttgggtgcat taaacggttt gccagttcac catcggtggt gaacagcagc
    17281 agaccacagg tattaacgtc caagcgaccc acggcaatcc agcgtgcgcc acgcagtttt
    17341 ggcagacggt caaacaccgt tggacgtcct tccggatcgt tacgggtaca caactcacct
    17401 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
    17461 aggtgaccat cgatacggat tttcaatcca ggggtaactt caacgcgatc gccgagttta
    17521 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcga
    17581 gaaccgtggc cggcacgcgc cagcactttc tgtagctttt cgctcataga gcttcctttt
    17641 ggtgtcgcct tcccaggcgt ctgatatgct tttgtgcaac agtgagtcag aacttacatc
    17701 tgactcactg atagtaaagt ctgtttgaac tcttcaggcc ttaagtaaac gactcgacgt
    17761 tttaactggc ctggctttga ggcgcaacat actacactat ctttatggtt aaagcttgtt
    17821 tcgattaaca tcagcttaat taccagccac caccgcctcc gccacccgcg ccgccgccag
    17881 caccaccacc ggaataactt cgccccaaca aactggtatt ggcccccaga ctcaggtgca
    17941 tctgtaatga gaacaaaacg ccactacggg caatttccgg tgcgctaaca gccgctgtga
    18001 gtgatggggt gatacgtgct acccattctc ttccaaggtt tgctgagact gcccaacctg
    18061 gcaacgcata atccagtttc cgtgtttccc ctttccgacg ccgcccactg tgcgttgccg
    18121 cctctttacg cgcaagatag ttaacgatag cttcaccgtg ggcataacgt tgttgtccag
    18181 tttgggtaaa ctttggcaga aaaatatacc ccatgccagt gagataaccg gtcaggaaaa
    18241 cagcgctaaa ataacccgct ggcatatacc agtaaaagaa tacatctccg acattgatat
    18301 agagagaata gaaggctacg cctgaacaaa tcagtggtaa aaacaaagtg atgattacac
    18361 gattaacgca aggcttgtca tcatcgcggg caaacaaaag ctcaagggaa caaaataaaa
    18421 aaggaagaag ccagcagatc ataataaaca tatcgcccac cagactcatt cctgcccagc
    18481 ctacaggact caggttaccg taaagaatga ccgccaatat tgccactccc ccccattgca
    18541 aaaaagtatc aggccggtaa aacgaagact ttttacgcgg cagataaaat ttctgcatcc
    18601 gtaaaaatgc cctgcgtaag actctgttgc gtctccccgt aagaacgacc ttatcgcctt
    18661 tacgaaataa cgcttccagt aataactgct ctgcgggcag taatacctca ttatttctgg
    18721 tcgcgccttc attaacacga atcaggattt gctggttctt atcatactga tcttccagat
    18781 gaatttttcc tttcacaatc agatttacga tatcggcaca aaacccttta tcatcgtaaa
    18841 ccagcttcgc atcgagacgt aacatgccgg gcgtgtaatc tggcggaatg acatcggttt
    18901 caatcacatc taccggcgta aattgcggcc tgcgcttcca gagatataac catccacaaa
    18961 ccaggaatag acacggaaac caaattaaca acgatgacgt ggagggtaaa agaagatggc
    19021 tgaaaatatt cgtcgtttgt ggagccgggg cattgctaag taacgagtga ggccaacggt
    19081 agagtaccgt gaaatcttca cgataaaacg gatcgctgga ttcaattctt ccgtcctcaa
    19141 ggatgcggcc atttcgatat gtgtcgccct cttctccagt aaagagagcg atttcgctaa
    19201 atggattacc cgcgatatca gggaacttga gtgaaaatcg ggtcttataa atttcaaacg
    19261 gccagttgtt accagtcacg ttccagatta gcaggtcaga gtcaccctca cgcaggaaag
    19321 catttttaac ctgataatga atttgataat catagtcacc ttgaggcaag aaactatgtt
    19381 tatcacccac gacaatactc attaacccga tatgccagtt aagatgccag ggttcaggaa
    19441 taccatcacg ggtgacgcta ataatgcgat agttcagcag cgccgcgtca ccatcaggct
    19501 gcatccatag tcgtggtaaa tcacgataaa atccgtgctt aatctgctga tttcgcgcct
    19561 gaaaatgaat gttttcttcg atgtcgagca ccccatccgg ttgcagggta gcctcaacat
    19621 caaagagcga gatcccttct tcataagaag ggatctccgt gtttttcgca taactcaacg
    19681 aagagaaaaa aaaagcaact atcaacagca aaatacagcg atatatccct gccataatct
    19741 tccatccgtg gaacgcaatt tgttataaga aaggcttcac atcacctacg ccttcacgca
    19801 ccacgaccgg cgtatcatca gtaagatcaa taaccgttgt tggtttctgc ccgagatagc
    19861 caccgtgaat aatcaaatcc acctgttttt ccagacggtc tttaatttct tccggatcgg
    19921 attcggtaaa ttcgctgcct ggcagcatta gcgaagtgga aagcatcggt tcgcccaatg
    19981 cttcaagcaa agcctgcgca atcgggttag aaggcacacg cataccgatg gttttgcgtt
    20041 tttcctgcaa cagacggcgt ggcacttctt tcgtcccttt caggatgaag gtgtagttac
    20101 ccggcgtgtt gtttttcatc aaacgaaacg caacgttatc gacaaatgaa taggtcgaga
    20161 gttcagaaag atcgcgacac atcagggtaa agttgtgacc gtccggcagc tggcgaatac
    20221 gacaaatacg ctccatcgcg tttttatctt caattttaca gccgagcgca tagccggaat
    20281 cggttggata aacaatcacc ccgcctttac gcacgatctc caccgcctgg ttgatcagac
    20341 gttgctgcgg gttatcagga tgaatataaa aaaactggct cataattccc tctctgtggt
    20401 gttctgcggc tgttcccata gctgccagac gccttcaacg cctgcgggca gccagagttt
    20461 acgacccagt tcgatccacg ggcatggctg atgaaaatca gatccttgtg atgcccataa
    20521 atgatgctga cgcgcaagtg tcgccagctg ggtacgttca ttgggcgatt gctggcactg
    20581 cgcgacttcc atcgcgtcgc cgtcgtgttc ggcaaaatgc gcaaccaatc ttttcagcca
    20641 tttagcacta agattgtacc gcccaggatg agctagcacc gccttaccgc cagaatgatg
    20701 aatgacatca atagcttgtt ctattgtaca ccactgtggc ggaacgtatc cggttttccc
    20761 gcgcgccaga tactttttaa agacatccgc cattgaactg gctttgccgc actcaacaag
    20821 gaaacgcgca aaatgagcgc gcgtcactgc gccgccctgc gccagtcgtt gtgcgccttc
    20881 cagcgcccca ggtatttgcg ctttttcaag tctttcggca atgagctgcg cccgctgatt
    20941 gcgccgttct gtctgctgcg cgaggaactc acacattagc agatgagtaa tatcaatatt
    21001 cagcccgaca atatgaattt catgattttc ccagaccgtg gaaatttcca cgccgggaat
    21061 aagattcagc gccagtcccg aacgtgaaat ttcctctctt gctggcgcga ttgcagctgt
    21121 ggtgtcatgg tcggtgatcg ccagggtgcc gacgcgcatc tcgactgcac ggtgcaccaa
    21181 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
    21241 tgcgtaattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
    21301 tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tcgaactagt
    21361 taactagtac gcaagttcac gtaaaaaggg tatcgacaat gaaagcaatt ttcgtactga
    21421 aaggttggtg gcgcacttcc tgaaacgggc agtgtattca ccatgcgtaa agcaatcaga
    21481 tacccagccc gcctaatgag cgggcttttt tttgaacaaa attagagaat aacaatgcaa
    21541 acacaaaaac cgactctcga actgctaacc tgcgaaggcg cttatcgcga caatcccacc
    21601 gcgctttttc accagttgtg tggggatcgt ccggcaacgc tgctgctgga atccgcagat
    21661 atcgacagca aagatgattt aaaaagcctg ctgctggtag acagtgcgct gcgcattaca
    21721 gctttaggtg acactgtcac aatccagtcc ctttccggca acggcgaagc cctgctgaca
    21781 ctactggata acgccctgcc tgcgggtgtg gaaaatgaac aattaccaaa ctgccgtgtg
    21841 ctgcgcttcc cccctgttag tctactgctg gatgaagacg cccgcttatg ctccctttcg
    21901 gtttttgacg ctttccgctt attgcagaat ctgttgaatg taccgaagga agaacgagaa
    21961 gccatgttct tcggcggcct gttctcttat gaccttgtgg cggggtttga agatttaccg
    22021 caactgtcag cggaaaataa ctgccctgat ttctgttttt atctcgctga aacgctgatg
    22081 gtgattgacc atcagaaaaa aagcacccgt attcaggcca gcctgtttgc tccgaatgaa
    22141 gaagaaaaac aacgtctcac tgctcgcctg aacgaactac gtcagcaact gaccgaagcc
    22201 gcgccgccgc tgccggtggt ttccgtgccg catatgcgtt gtgaatgtaa ccagagcgat
    22261 gaagagttcg gtggcgtagt gcgtttgttg caaaaagcga ttcgcgctgg agaaattttc
    22321 caggtggtgc catctcgccg tttctctctg ccctgcccgt caccgctggc ggcctattac
    22381 gtgctgaaaa agagtaatcc cagcccgtac atgtttttta tgcaggataa tgatttcacc
    22441 ctgtttggcg cgtcgccgga aagttcgctc aagtatgacg ccaccagccg ccagattgag
    22501 atctacccga ttgccggaac acgtccacgc ggtcgtcgcg ccgatggctc gctcgacagg
    22561 gaccttgaca gccgtattga actggaaatg cgtaccgacc ataaagaact ttcagaacat
    22621 ctgatgctgg tagatctcgc ccgaaatgac ctggcgcgca tttgcacccc cggcagccgc
    22681 tacgttgccg atctcaccaa agtagaccgt tattcctatg tgatgcacct cgtctcccga
    22741 gtggtcgggg aactgcgcca cgatctcgac gccctgcatg cttaccgcgc ctgtatgaat
    22801 atgggtacgt taagcggtgc gccgaaagta cgcgctatgc agttaattgc cgaggctgaa
    22861 ggtcgtcgcc gcggcagcta cggcggcgcg gtaggttatt tcaccgcgca tggcgatctc
    22921 gacacctgca ttgtgatccg ctcggcgctg gtggaaaacg gtatcgccac cgtgcaagcc
    22981 ggtgctggcg tagtccttga ttctgttccg cagtcggaag ccgacgaaac ccgtaataaa
    23041 gcccgcgctg tactgcgcgc tattgccacc gcgcatcatg cacaggagac tttctgatgg
    23101 ctgacattct gctgctcgat aatatcgact cttttacgta caacctggca gatcagttgc
    23161 gcagcaatgg gcataacgtg gtgatttacc gcaaccatat tccggcgcaa accttaattg
    23221 aacgcctggc gacgatgagc aatccggtgc tgatgctttc tccaggaccc ggtgtgccga
    23281 gcgaagctgg ttgtatgcct gaactgctta cccgcctgcg cggtaagctg cctattattg
    23341 gcatttgcct tggtcatcag gcaattgtcg aagcttacgg gggctatgtc ggtcaggcgg
    23401 gcgaaattct ccacggtaaa gcctccagca ttgaacacga cggtcaggcg atgtttgccg
    23461 gattaacaaa cccgctgccg gtggcgcgtt atcactcgct ggttggcagt aatattccgg
    23521 ccggtttaac catcaacgcc cattttaatg gcatggtgat ggcggtgcgt cacgatgcag
    23581 atcgcgtttg cggtttccag tttcatccag agtccattct tactacccaa ggtgctcgcc
    23641 tgctggaaca aacgctggcc tgggcgcagc agaaactaga gccaaccaac acactgcaac
    23701 cgattctgga aaaactgtat caggcgcaga cgcttagcca acaagaaagt caccagctgt
    23761 tttcagcggt ggtgcgtggc gagctgaagc cggaacaact ggcggcggcg ctggtgagca
    23821 tgaaaattcg cggtgagcac ccgaacgaga tcgctggggc agcaaccgcg ctactggaaa
    23881 acgcagcgcc gttcccgcgc ccggattatc tgtttgctga tatcgtcggt actggcggtg
    23941 acggcagcaa cagtatcaat atttctaccg ccagtgcgtt tgtcgccgcg gcctgtgggc
    24001 tgaaagtggc gaaacacggc aaccgtagcg tctccagtaa atccggctcg tcggatctgc
    24061 tggcggcatt cggtattaat cttgatatga acgccgataa atcgcgccag gcgctggatg
    24121 agttaggcgt atgtttcctc tttgcgccga aatatcacac cggattccgc cacgcgatgc
    24181 cggttcgcca gcaactgaaa acccgcaccc tgttcaatgt gctggggcca ttgattaacc
    24241 cggcgcatcc gccgctggcg ttaattggtg tttatagtcc ggaactggtg ctgccgattg
    24301 ccgaaacctt gcgcgtgctg gggtatcaac gcgcggcggt ggtgcacagc ggcgggatgg
    24361 atgaagtttc attacacgcg ccgacaatcg ttgccgagct gcacgatggc gaaattaaga
    24421 gctatcagct caccgcagaa gactttggcc tgacacccta ccaccaggag caactggcag
    24481 gcggaacacc ggaagaaaac cgtgacattt taacacgctt gttacaaggt aaaggcgacg
    24541 ccgcccatga agcagccgtc gctgcgaacg tcgccatgtt aatgcgcctg catggccatg
    24601 aagatctgca agccaatgcg caaaccgttc ttgaggtact gcgcagtggt tccgcttacg
    24661 acagagtcac cgcactggcg gcacgagggt aaatgatgca aaccgtttta gcgaaaatcg
    24721 tcgcagacaa ggcgatttgg gtagaagccc gcaaacagca gcaaccgctg gccagttttc
    24781 agaatgaggt tcagccgagc acgcgacatt tttatgatgc gctacagggt gcgcgcacgg
    24841 cgtttattct ggagtgcaag aaagcgtcgc cgtcaaaagg cgtgatccgt gatgatttcg
    24901 atccagcacg cattgccgcc atttataaac attacgcttc ggcaatttcg gtgctgactg
    24961 atgagaaata ttttcagggg agctttgatt tcctccccat cgtcagccaa atcgccccgc
    25021 agccgatttt atgtaaagac ttcattatcg acccttacca gatctatctg gcgcgctatt
    25081 accaggccga tgcctgctta ttaatgcttt cagtactgga tgacgaacaa tatcgccagc
    25141 ttgccgccgt cgcccacagt ctggagatgg gtgtgctgac cgaagtcagt aatgaagagg
    25201 aactggagcg cgccattgca tcgggggcaa aggtcgttgg catcaacaac cgcgatctgc
    25261 gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc gccgaaactg gggcacaacg
    25321 tgacggtaat cagcgaatca ggcatcaata cttacgctca ggtgcgcgag ttaagccact
    25381 tcgctaacgg ttttctgatt ggttcggcgt tgatggccca tgacgatttg aacgccgccg
    25441 tgcgccgggt gttgctgggt gagaataaag tatgtggcct gacgcgtggg caagatgcta
    25501 aagcagcgta tgacgcgggc gcgatttacg gtgggttgat ttttgttgcg acatcaccgc
    25561 gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc ggcaccgttg cagtatgttg
    25621 gcgtgttccg caatcacgat attgccgatg tgttggacaa agctaaggtg ttatcactgg
    25681 cggcagtgca actgcatggt aatgaagatc aacaatatat cgacacattg cgtggcgcgc
    25741 tgcccgcaaa cattgccatc tggaaagcat taagcgtcgg tgaaaccctg cccgcccgcg
    25801 agcttcagca cgttgataaa tatgttttag acaacggcca gggtggaagc gggcaacgtt
    25861 tcgactggtc actgttaaac ggtcaatcgc ttggcaacgt tctgctggcg gggggcttag
    25921 gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc cggacttgat tttaattctg
    25981 ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt ggcctcggtt ttccagacgc
    26041 tgcgcgcata ttaaggaaag gaaaaatgac aacattactt aacccctatt ttggtgagtt
    26101 tggcggcatg tacgtgccac aaatcctgat gcctgctctg cgccagctgg aagaagcttt
    26161 tgtcagcgcg caaaaagatc ctgaatttca agcccagttc aacgacctac tgaaaaacta
    26221 tgccgggcgt ccaaccgcac tgaccaaatg ccagaacatt acggctggga cgaaaaccac
    26281 gctgtatctg aagcgcgaag atttgctgca cggcggcgcg cataaaacta accaggtgct
    26341 cggtcaggct ttactggcga agcggatggg taaaaccgaa atcatcgccg aaaccggggc
    26401 cggtcagcat ggcgtggcgt cggcccttgc cagcgccctg ctcggcctga aatgccgtat
    26461 ttatatgggt gccaaagacg ttgaacgcca gtcgcctaac gtttttcgta tgcgcttaat
    26521 gggtgcggaa gtgatcccgg tacatagcgg ttccgccacg ctgaaagatg cctgtaatga
    26581 ggcgctacgc gactggtccg gcagttatga aaccgcgcac tatatgctgg gcaccgcggc
    26641 tggcccgcat ccttacccga ccattgtccg tgagtttcag cggatgattg gcgaagaaac
    26701 caaagcacag attctggaaa gagaaggtcg cctgccggat gccgttatcg cctgtgttgg
    26761 cggcggttcg aatgccatcg gtatgtttgc agatttcatc aacgaaaccg acgtcggcct
    26821 gattggtgtg gagcctggtg gtcacggtat cgaaactggc gagcacggcg caccgctaaa
    26881 acatggtcgc gtgggcattt atttcggtat gaaagcgccg atgatgcaaa ccgaagacgg
    26941 gcagattgaa gaatcttact ccatctccgc cggactggat ttcccgtctg tcggcccaca
    27001 acacgcgtat cttaacagca ctggacgcgc tgattacgtg tctattaccg atgaagaagc
    27061 ccttgaagcc ttcaaaacgc tctgcctgca tgaagggatc atcccggcgc tggaatcctc
    27121 ccacgccctg gcccatgcgt tgaaaatgat gcgcgaaaac ccggaaaaag agcagctact
    27181 ggtggttaac ctttccggtc gcggcgataa agacatcttc accgttcacg atattttgaa
    27241 agcacgaggg gaaatctgat ggaacgctac gaatctctgt ttgcccagtt gaaggagcgc
    27301 aaagaaggcg cattcgttcc tttcgtcacg ctcggtgatc cgggcattga gcagtcgctg
    27361 aaaattatcg atacgctaat tgaagccggt gctgacgcgc tggagttagg catccccttc
    27421 tccgacccac tggcagatgg cccgacgatt caaaacgcca cactgcgtgc ctttgcggca
    27481 ggtgtgaccc cggcgcagtg ctttgagatg ctggcactca ttcgccagaa gcacccgacc
    27541 attcctatcg gccttttgat gtatgccaat ctggtgttta acaaaggcat tgatgagttt
    27601 tatgcccagt gcgaaaaagt cggcgtcgat tcggtgctgg ttgccgatgt gccagttgaa
    27661 gagtccgcgc ccttccgcca ggccgcgttg cgccataatg tcgcacctat ctttatctgc
    27721 ccgccgaatg ccgatgacga cttgctgcgc cagatagcct cttacggtcg tggttacacc
    27781 tatttgctgt cgcgtgcggg cgtgaccggc gctgaaaacc gcgccgcgtt acccctcaat
    27841 catctggttg cgaagctgaa agagtacaac gctgcacctc cattgcaggg atttggtatt
    27901 tccgccccgg atcaggtaaa agcagcgatt gatgcaggag ctgcgggcgc gatttctggt
    27961 tcggccattg ttaaaatcat cgagcaacat attaatgagc cagagaaaat gctggcggca
    28021 ctgaaagttt ttgtacaacc gatgaaagcg gcgacgcgca gttaatccca cagccgccag
    28081 ttccgctggc ggcattttaa ctttctttaa cgaagctgga aaaatcctaa attcatttaa
    28141 tatttatctt tttaccgttt cgcttacccc tgtcgaacgt caacttacgt catttttccg
    28201 cccaacagta atataatcaa acaaattaat cccgcaacat aacaccagta aaatcaataa
    28261 ttttctctaa gtcacttatt cctcaggcaa ttgttaatat atcaagaacg ttcctcataa
    28321 tatatttttc cactttcttc tcgttacgct taatttgact aattctcatt agcgactaat
    28381 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    28441 ataacatggc cgaacatcgt ggtggttcag gaaatttcgc cgaagaccgt gagaaggcat
    28501 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    28561 gcgcatctga agcgggtaaa aaaggcggtc aacaaaacgg tggtaataaa tcaggtaaat
    28621 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    28681 tggctcgcag tgattccact ggaggaaaat atgaatatga agaccatgga agatgtattt
    28741 attcacctac tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    28801 ctcgcaagag caacatcaaa cgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    28861 acacatggac agattgaacg tattgatcaa gttgtggaat cagaatcgaa tctgaaaatt
    28921 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    28981 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    29041 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    29101 aaagcagcga agcttctgaa agagtccctg gaagaagaaa aggccaccga catcaaactg
    29161 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaac
    29221 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    29281 gactggttac gtgacgccca tgcaatggaa aagcaagccg aatctatgct tgaatccatg
    29341 gccagccgta tagataatta tcctgaacta cgtgctcgta ttgaacaaca tcttagtgaa
    29401 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    29461 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    29521 ccttctgatg aaatagtcaa aggctctatt agcggatatg tcttcgagca atttgaaatc
    29581 gcctgttaca cctctctatt agcagcagca aaaaatgccg gtgatacagc ctcaattccg
    29641 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    29701 ccgcaaacaa ctgagaaatt tttaattcgc tctgaaactg atggcgtaga agcgaagaaa
    29761 taataccctt tatgccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    29821 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatca
    29881 tgttgctgtg caccgcccag tttataattg gcggtggtat cgatatccat gtaccacact
    29941 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    30001 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    30061 ccattatcaa agaaggtggt gtagttaata cctgccccaa cataaggacg gaatttgctg
    30121 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatgatg aacggttgca
    30181 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    30241 acaccaatgt tgtcggtcgc catataagta aacgtcaggc ccagttgagt gttattggtc
    30301 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgtagg acgtacggtt
    30361 gcagaacctg cacgcataaa aaattctcct gcttcatgcg caaaggcact gccagagaga
    30421 agtgttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    30481 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    30541 ttacatatga taattcaccc tttattgatc tggattaatt tagaagttgc attgagaatc
    30601 ctggttaatg tgatttaaat caatttttaa atattttcgc tacgcaaata ttaacttgtt
    30661 cgccacttgc cgcggtttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    30721 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    30781 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    30841 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    30901 gctatgtgcg tttatcacaa tggtgttagg gcatgttttc tcacccagtg atgcacagct
    30961 tgcgcagctc aatgacggcg tgcccgttgg cggcagtagt gggttgttcg acctggttca
    31021 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcat ccactttttc
    31081 aggattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    31141 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgccaaa
    31201 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    31261 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgt tggtacagga
    31321 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    31381 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    31441 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    31501 cctgatttca gccgtattgc tcatctatct gttccgtctg tatatgttga ttcgccaata
    31561 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    31621 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc ggcgctgatc
    31681 gtcgccacgg cgattgtgct tatatatagc tgggttcgct ttcgtaaggt tgagaagatg
    31741 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    31801 gatgagttta taaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    31861 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgggtaa agaactcacg
    31921 ctgccgcaac cggtatggtc gaagctgaat ctggcctggg ctgttttctt tatcctttgc
    31981 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    32041 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    32101 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    32161 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    32221 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    32281 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    32341 cacaaatgga tattggcggc gctattctgg cgaaagaaat tgcccacggt cgcgtagtga
    32401 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    32461 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    32521 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    32581 agtatgtcgc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taataatcaa
    32641 aagcctccgg tcggaggctt ttgactttat gcttactgaa tttcggtggt gccgttaatt
    32701 ttaaagagga tgttcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    32761 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    32821 acgttatcca cgcgaccatc cggcgtgaca tcaaatttaa ctttaacctg cccttcaatg
    32881 cgcaatgcct gtgcccgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    32941 gaagccacac tggtaaccgg cttgctggtc gcagccgttg ccgtacttga tgtcgggcgt
    33001 gccggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    33061 tttggctgtt cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    33121 ttttcaatga ccaccggagc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    33181 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    33241 gcaaccatcg tgacagaaat tggctgcgca ggcgcaggta gttcaataac ctgatgtacc
    33301 gaggtataga gcagacccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    33361 caggggaagc ggcgaggtaa atcaagggtc attgaagtca taatcatttc agtagaaaaa
    33421 ccaggtctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    33481 cagtgaaaat gtcattttcg gcgtaattga ccttacttta tgcatggtct taacaaagac
    33541 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    33601 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    33661 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    33721 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    33781 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    33841 gatgctgacc cgtacgtagc ggcaggtgtc tatgaacacg tttcagtgaa accatttaag
    33901 aaagtgttct gaatagagat tcaatattta agggcacggt ttttgcaacc gtgttcttgt
    33961 ttggcctttc cttatagccg gtcagtgcat tcaccaccac aagcagataa taactttatc
    34021 tgtatttctt tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt
    34081 gctcgctcaa aacctttgat tactgcggat aaagtcatct actattgttt aacacattca
    34141 cgcgctgcat gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta
    34201 cagaggtatc agtgagtcac tgggctacat tcaaacaaac agcaacaact ttatgggtga
    34261 ccctacggca cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa
    34321 caatctatgg tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt
    34381 tggatctctc cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga
    34441 actccattgg cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt
    34501 tgatagcgct aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt
    34561 gcatttttac gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg
    34621 cagccgggac aatttttgca tttattagtt tcacgacgtt actgttttac tccacctacg
    34681 gtgcgcttta tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct
    34741 atttttcgat agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat
    34801 cagcacgatt gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta
    34861 tgacctcaat ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca
    34921 atcatacaat gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca
    34981 atacgatcct gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg
    35041 aagatgatat caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg
    35101 acagtaatga cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg
    35161 cgctgagtga taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca
    35221 gtgatgtcaa aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg
    35281 tacatccgga tatcattctc tcaccgcaac tatttggcag cgaaattctg gcgcgagttt
    35341 taaatggtga agagattaat aatgatatgc tcgtttcaat gttgttgaac tcaggtcatg
    35401 gtattttcag cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcggcgcaaa
    35461 aatagagttt aaagacggaa gagtaaactt attgggcacc gcaatggtgc ccagtacatt
    35521 tagatattgt agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg
    35581 atgctgtgga tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg
    35641 ctagcctgac gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact
    35701 actctatgca ataacagaat ggtcattata cccccgcccc cgacgctgac cagtgctttt
    35761 cccgcgtttt tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa
    35821 aaggatttct aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga
    35881 tactggttgc tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc
    35941 tccgcgatgg cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat
    36001 cttgccgttg gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg
    36061 ccttccaccg caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa
    36121 aatagcagtg tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg
    36181 gtcaaaggga atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc
    36241 cgcgacatcc agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc
    36301 ggcatggcgg accaggtggc tgaatcatta atggcggctg cgcgacgcgg cattcattgc
    36361 cgattgatgc tcgactccgc cgggagtgta gcttttttcc gcagcccgtg gcccgagcta
    36421 atgcgtaatg ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt
    36481 ttacgccgta tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg
    36541 tacaccggca gcatgaatat ggtcgaccct cgctacttca aacaagatgc gggcgtaggg
    36601 caatggattg atctaatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt
    36661 tattcctgcg actgggagat tgaaacaggg aaacgtattc taccgccacc acctgatgtc
    36721 aatattatgc cgtttgagca ggccagcggt cataccattc acacaattgc ttctggcccc
    36781 ggctttccgg aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa
    36841 tatttgatca tgaccacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc
    36901 acggcggcgc agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg
    36961 ctggtcggct gggccagtcg cgcattcttt acagaactgc ttgctgctgg ggttaaaatt
    37021 tatcagtttg aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt
    37081 ctggttggca ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg
    37141 gcaatcgacg ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg
    37201 cgttcacgtc tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc
    37261 gagcgactgt tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca
    37321 ggtagtgact atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca
    37381 ggcctacgat atttttctcg aactggctgc cgacaatctc gatccagctg atgttctgct
    37441 gttcaatctt cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg
    37501 gcaggaacat gttgatttcg atttgaaccc agactttttt gccgaagtgg tgattggtct
    37561 ggcggacagt gaagatggtg aaatcaacga tgttttcgcc cgcattctgt tatgccgcga
    37621 aaaagatcac aaactttgcc atatcatctg gcgggaataa ataatcactt tcggcgtgaa
    37681 aaagggctga caactgtcag ccctttttgt gtttaaagcg gatcgacttt caggcaagaa
    37741 acggcgtgtc tgaaactccc ctccagcaca ggatgtgttt tggcacactc cggaccggca
    37801 atcgggcaac gggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc
    37861 ccttccagta actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc
    37921 gacatcaatg ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc
    37981 agttctaccg catggccgag atacatcact aacacacgat cggaaatgtg ttttaccacg
    38041 gccaggtcgt gagcgataaa aattaatgac aatcccatct cacgttgtaa ctgctggagc
    38101 agattgacca cctgcgcctg aattgacacg tccagcgccg acaccggctc atcacagata
    38161 atcagcttcg gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg
    38221 gagaactcat gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc
    38281 gccttcacgc gctcgcgaac ttcctggcgt gacattttcg gatgataggt acgcagcggt
    38341 tcagcgataa tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa
    38401 atcatctgaa tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct
    38461 ttacctaacc aggcaacatg accgtcggtc gccttgacca aaccgatgat agcgcgagca
    38521 aaggtggact taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg
    38581 cgaagagtga caccatcgac ggctttgagc gttttcggcg gttgccagaa ccactgtttg
    38641 ccatctttga tttcaaagtg cactttcaga tcggcgattt caaggaggac ttttcttcct
    38701 tcggttacag cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag
    38761 gcgtaaactc ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg
    38821 gctggaacgg gcaacctttc ggtaatcgca gcaggtttgg cggattaccc ggaatagtca
    38881 acattgtttc accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag
    38941 gatgaacggg ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt
    39001 acattaccag cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtgatcataa
    39061 tgatggcggt attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta
    39121 cggtgacgtc cagcgcagta gtgggttcat ccgcaatcag cagcttaggc cgacatagca
    39181 atgccatcgc aatcatgact cgctgacgca tgccgccaga aaattcgtgc gggtacattt
    39241 tcatgcgttt acgcgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa
    39301 acgcctcagc tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga
    39361 cgcgcatata gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag
    39421 cgcgcagttt attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg
    39481 ccgatccgcc aatgcgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg
    39541 atttacccga accagactca cctacaatgc ccagcgtttc tccggcacgt agggaaaaat
    39601 tcaaatcatt gaccgccgtg acgtcgccgt ccggggtact aaaggtgaca cgcaaatctt
    39661 tcacgttcag cagtgcgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta
    39721 cgctcatggc tgcactcctt aacgatcttt cgggtcgagg gcatcacgca agccatcgcc
    39781 gataaagttg aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acagtaacca
    39841 tggagagact tccatcgagt tcgcgccatc actcagcaat gccccccagc tgcttaacgg
    39901 ctcttgcgta cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac
    39961 cagtagcgat gcgtagacca ccaccacacc gagtacgttc ggcacaatgt ggcgaataac
    40021 aatgcccggc gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag
    40081 actcagggtt tgcccacgca caatacgagc catatccagc caggaaacca tgccaatcgc
    40141 cacgaaaatc agcaggatgt tttggccgaa aaaggtcacc agcaaaatga cgaagaacat
    40201 gaatgggaag gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc
    40261 cagataaccg gaaagcgaac cataaagtgt ccccacaacc actgccacca gtgccgcagc
    40321 aacaccgacc atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc
    40381 ggatgagtca gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc
    40441 ccagtcagta tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc
    40501 gatcagcacc agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg
    40561 tgcgtcctgc cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt
    40621 ctcgctgttt ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg
    40681 tcgataaccg catatagcac atcgacaatg gcattaaaca aaatggttaa cgcaccaacc
    40741 aggatggtca ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt
    40801 tgcccaatcc ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg
    40861 acaaatgcgg ggcccatata ggagagcacg ggtaacagag caggttttaa tgcgtggcgt
    40921 aaaatgatcc gccgcatagg taaccctttc gcccgggcag tacgaataaa attggagtgt
    40981 aatacttcaa tcatagaacc acgggtaata cgcgcaatac tggcgatata ggcgagtgac
    41041 aacgccacca ttggcaatat catgaattta agcgccccac cattccagcc accgcccggc
    41101 agccaatgta aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg
    41161 ataacaaccc cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt
    41221 aatgcggcaa taacgccagc actaacaccc agtattaccg caaggaaaaa tgctgcggct
    41281 cccagtttgg cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta
    41341 tatttaaacg atggaccgaa atcgccatgc gccagttgtt tcaggtagct gaaatactgt
    41401 gtcatgatcg gatcattaag atgatatttc gcttcgatat tggccattac ttccggcggt
    41461 aaagtacgtt cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata
    41521 gtaataagaa taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac
    41581 attgccctac cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc
    41641 aggacactcc tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtata
    41701 ggtattatcc agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc
    41761 attcacgtag taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt
    41821 agtgtacaga gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct
    41881 gtcaaaggcc gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt
    41941 caggaaggaa gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt
    42001 gccctggtga cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac
    42061 accaatgttt ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcgga
    42121 ggtgttatac agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag
    42181 cagttttttc gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt
    42241 caattttgcg ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc
    42301 tttcacttta ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg
    42361 cacatcgttg aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acagatatgg
    42421 atcaacgtga acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat
    42481 gttgttatac gtcatgtcga tttcaccact acggtagcgg ttaacatcgg taacttcaga
    42541 agcaataggc aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg
    42601 gctgcgttca agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt
    42661 ggtgacgata ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg
    42721 caccggtgaa gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa
    42781 ggtgacttct aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt
    42841 cccttcaaga atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg
    42901 agaagcagtg ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac
    42961 tggcgtgcca tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc
    43021 tttattatcc caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag
    43081 taagccttca aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg
    43141 atctaatgac tgaacttctg aaccattgtt acgtaccagc gtttgttttt ccgccagtgt
    43201 gacgcctgcg ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac
    43261 gccagctgct actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt
    43321 ataattactg gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga
    43381 gactttctgc tgtcaggtta ctttatcgtt acggattgct atcaccgact ttatttattc
    43441 tggtgattca gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat
    43501 tattctttcg cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgcctg
    43561 gcggttgccc ggaaagtacc aaatgcgctt cttactcgcc aatacatttt gcaaaattgt
    43621 taagcaattc tctttt
//
LOCUS       DAPGRN010000028        42428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPGRN010000028 DAPGRN010000000
VERSION     DAPGRN010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.028"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            195..737
                     /gene="ecpR"
                     /locus_tag="RHW33_001664"
                     /gene_synonym="matA"
     CDS             195..737
                     /gene="ecpR"
                     /locus_tag="RHW33_001664"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV1645183.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            812..1399
                     /gene="ecpA"
                     /locus_tag="RHW33_001665"
                     /gene_synonym="matB"
     CDS             812..1399
                     /gene="ecpA"
                     /locus_tag="RHW33_001665"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV1645184.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1457..2125
                     /gene="ecpB"
                     /locus_tag="RHW33_001666"
                     /gene_synonym="yagY"
     CDS             1457..2125
                     /gene="ecpB"
                     /locus_tag="RHW33_001666"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV1645185.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAAKGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2151..4676
                     /gene="ecpC"
                     /locus_tag="RHW33_001667"
                     /gene_synonym="yagX"
     CDS             2151..4676
                     /gene="ecpC"
                     /locus_tag="RHW33_001667"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV1645186.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGGDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4666..6309
                     /gene="ecpD"
                     /locus_tag="RHW33_001668"
     CDS             4666..6309
                     /gene="ecpD"
                     /locus_tag="RHW33_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV1645187.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPASEFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINTCQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNISI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWRPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6278..6988
                     /gene="ecpE"
                     /locus_tag="RHW33_001669"
     CDS             6278..6988
                     /gene="ecpE"
                     /locus_tag="RHW33_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV1645188.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPQDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7301..7630
                     /locus_tag="RHW33_001670"
     CDS             7301..7630
                     /locus_tag="RHW33_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV1645189.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7877..8490)
                     /locus_tag="RHW33_001671"
                     /pseudo
     CDS             complement(7877..8490)
                     /locus_tag="RHW33_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
     gene            complement(8537..8746)
                     /locus_tag="RHW33_001672"
     CDS             complement(8537..8746)
                     /locus_tag="RHW33_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645190.1"
                     /translation="MMMGAGLLLELYDAKTDKETCDCQLCITLQIYVTFNIKIGRNDG
                     AYILWRLDNHLVIQNNTVLYIYLVL"
     gene            8908..9597
                     /gene="paoA"
                     /locus_tag="RHW33_001673"
     CDS             8908..9597
                     /gene="paoA"
                     /locus_tag="RHW33_001673"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV1645191.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAAAAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLSGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9594..10550
                     /gene="paoB"
                     /locus_tag="RHW33_001674"
     CDS             9594..10550
                     /gene="paoB"
                     /locus_tag="RHW33_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV1645192.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIIAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10547..12745
                     /gene="paoC"
                     /locus_tag="RHW33_001675"
                     /pseudo
     CDS             10547..12745
                     /gene="paoC"
                     /locus_tag="RHW33_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
     gene            12755..13711
                     /gene="paoD"
                     /locus_tag="RHW33_001676"
     CDS             12755..13711
                     /gene="paoD"
                     /locus_tag="RHW33_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV1645193.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGCDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            complement(13890..15017)
                     /locus_tag="RHW33_001677"
     CDS             complement(13890..15017)
                     /locus_tag="RHW33_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645194.1"
                     /translation="MTLCVFVLIASEFMPVSLLTPITRDLGVTEGLAGQGIAISGALA
                     VLTSLTLSTLAGKMNRKFLLLEMTVLMAVSGLIIALASNYLMYMVGRAMIGVAIGGFW
                     SMSAATAIRLVPQHQVTRALAIFNAGNALATVVAAPLGSYLGATVGWRGAFLCLVPIA
                     VVAFIWQCISLPSMDANKSHASFGAVFRLFSRRMVVVGMIACGLFFMGQFALFTYVRP
                     FLESVTRVNSLGLSLILLIIGVAGFIGTLVVSTFLNRKFYPTLMAIPGLMAVIAIGLM
                     LTGHHVWTVSLLLGLWGMLATAAPTGWWTWIARTLPDNAEAGGGLMVAVIQLSIALGS
                     TAGGIVFDRLGWQSTFAMSSVLLLCAGVLTFVTARQKAGAL"
     gene            complement(15159..16217)
                     /locus_tag="RHW33_001678"
     CDS             complement(15159..16217)
                     /locus_tag="RHW33_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1645195.1"
                     /translation="MSYADSTNPNAPVSMTDKWDKTFAESQKVDHRKVSFPNRYGITL
                     VGDLYLPKDRGDRKLAAIAVSGPFGAVKEQSSGLYAQTLAERGFVTLAFDPSYTGESG
                     GYPRNVASPDINTEDFSAAVDFLGLQKEVDRNRIGLLGICGWGGMALNDAAMDTRVKA
                     VATSVMYDMSRAMGHGVGDGEDRYSTADRRAVLQYLNEQRWKDAQSGTFAHGGHDINV
                     DSNGKVRAGERILPETLPANPHPVLKEFFDYYRMPRGFHERSVNSTGAWTATMPLSFM
                     NMPLLSYASEITIPTLIVTGEKAHSRYFAEDAYKVVGSNDKELVVVPGANHVDLYDNV
                     AGKIPFAKFEQFFQTKLK"
     gene            16482..17366
                     /locus_tag="RHW33_001679"
     CDS             16482..17366
                     /locus_tag="RHW33_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1645196.1"
                     /translation="MAKRENYNELYLFMQVVREGSFTAAAQRLGLAQSGVSRSVRELE
                     ERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINAS
                     QHAIDKCLLPKLAVFKQRYPDIRLELINESRFVDIIEERFDAGVRLGPEVGQGMVAVR
                     ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHR
                     PEGQWALSDSYMEAEAARLGLGLAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYY
                     PHRNVSPALRMVIDTLKI"
     gene            complement(18068..18343)
                     /locus_tag="RHW33_001680"
     CDS             complement(18068..18343)
                     /locus_tag="RHW33_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645197.1"
                     /translation="MPMYIKLILSIVRRINMNTICSLIVVVALSLLSFSSLAKTITAV
                     GSTINSTEKNFFTGRKQGKSYKILGAFFKNRVYMIAKLTPVSKNDAS"
     gene            complement(18507..19229)
                     /locus_tag="RHW33_001681"
     CDS             complement(18507..19229)
                     /locus_tag="RHW33_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1645198.1"
                     /translation="MDKVILITGASSGIGEGIARELGTTGAKTLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQVALDSWGRVDVLINNAGVMPLSPLAAGKQNE
                     WELTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
     gene            19328..20227
                     /locus_tag="RHW33_001682"
     CDS             19328..20227
                     /locus_tag="RHW33_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1645199.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGHLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     KQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            complement(20354..20429)
                     /locus_tag="RHW33_001683"
     tRNA            complement(20354..20429)
                     /locus_tag="RHW33_001683"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20394..20396),aa:Thr,seq:cgt)
     gene            complement(20544..21797)
                     /gene="proA"
                     /locus_tag="RHW33_001684"
     CDS             complement(20544..21797)
                     /gene="proA"
                     /locus_tag="RHW33_001684"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV1645200.1"
                     /translation="MLEQMGIAAKQASYKLAQLSCREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(21809..22912)
                     /gene="proB"
                     /locus_tag="RHW33_001685"
     CDS             complement(21809..22912)
                     /gene="proB"
                     /locus_tag="RHW33_001685"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV1645201.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            23199..24254
                     /gene="phoE"
                     /locus_tag="RHW33_001686"
     CDS             23199..24254
                     /gene="phoE"
                     /locus_tag="RHW33_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV1645202.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(24293..24694)
                     /gene="crl"
                     /locus_tag="RHW33_001687"
     CDS             complement(24293..24694)
                     /gene="crl"
                     /locus_tag="RHW33_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV1645203.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(24752..25996)
                     /gene="frsA"
                     /locus_tag="RHW33_001688"
     CDS             complement(24752..25996)
                     /gene="frsA"
                     /locus_tag="RHW33_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV1645204.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(26088..26546)
                     /gene="gpt"
                     /locus_tag="RHW33_001689"
     CDS             complement(26088..26546)
                     /gene="gpt"
                     /locus_tag="RHW33_001689"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV1645205.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            26807..28264
                     /gene="pepD"
                     /locus_tag="RHW33_001690"
     CDS             26807..28264
                     /gene="pepD"
                     /locus_tag="RHW33_001690"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV1645206.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPTK"
     gene            complement(28321..28842)
                     /locus_tag="RHW33_001691"
                     /pseudo
     CDS             complement(28321..28842)
                     /locus_tag="RHW33_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(28841..29044)
                     /locus_tag="RHW33_001692"
                     /pseudo
     CDS             complement(28841..29044)
                     /locus_tag="RHW33_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            complement(29290..29742)
                     /locus_tag="RHW33_001693"
     CDS             complement(29290..29742)
                     /locus_tag="RHW33_001693"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1645207.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASEHYSPQQIAVWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            complement(29739..30794)
                     /gene="dinB"
                     /locus_tag="RHW33_001694"
     CDS             complement(29739..30794)
                     /gene="dinB"
                     /locus_tag="RHW33_001694"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV1645208.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(30865..31650)
                     /gene="lafU"
                     /locus_tag="RHW33_001695"
     CDS             complement(30865..31650)
                     /gene="lafU"
                     /locus_tag="RHW33_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV1645209.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            31622..33334
                     /locus_tag="RHW33_001696"
                     /pseudo
     CDS             31622..33334
                     /locus_tag="RHW33_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(33543..34040)
                     /gene="rayT"
                     /locus_tag="RHW33_001697"
     CDS             complement(33543..34040)
                     /gene="rayT"
                     /locus_tag="RHW33_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HDV1645210.1"
                     /translation="MSEYRRYYLKGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKR
                     ARPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVCDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(34216..34965)
                     /locus_tag="RHW33_001698"
     CDS             complement(34216..34965)
                     /locus_tag="RHW33_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDV1645211.1"
                     /translation="MSLPSIPSFVLSGLLLICLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDKGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            35175..35435
                     /gene="dinJ"
                     /locus_tag="RHW33_001699"
     CDS             35175..35435
                     /gene="dinJ"
                     /locus_tag="RHW33_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HDV1645212.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNKLTIQSIKNSEAGVGVHKAKDADDLFDKLGI"
     gene            35438..35716
                     /gene="yafQ"
                     /locus_tag="RHW33_001700"
     CDS             35438..35716
                     /gene="yafQ"
                     /locus_tag="RHW33_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV1645213.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNILPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLRFERTGTHAALFG"
     gene            35872..36612
                     /locus_tag="RHW33_001701"
     CDS             35872..36612
                     /locus_tag="RHW33_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV1645214.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(36583..37350)
                     /locus_tag="RHW33_001702"
     CDS             complement(36583..37350)
                     /locus_tag="RHW33_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV1645215.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(37556..38134)
                     /gene="lpcA"
                     /locus_tag="RHW33_001703"
     CDS             complement(37556..38134)
                     /gene="lpcA"
                     /locus_tag="RHW33_001703"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV1645216.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            38374..40818
                     /gene="fadE"
                     /locus_tag="RHW33_001704"
     CDS             38374..40818
                     /gene="fadE"
                     /locus_tag="RHW33_001704"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV1645217.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKDLGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(40861..41307)
                     /gene="ivy"
                     /locus_tag="RHW33_001705"
     CDS             complement(40861..41307)
                     /gene="ivy"
                     /locus_tag="RHW33_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV1645218.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            41488..42258
                     /gene="yafV"
                     /locus_tag="RHW33_001706"
     CDS             41488..42258
                     /gene="yafV"
                     /locus_tag="RHW33_001706"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV1645219.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWRDADEFRLR"
     gene            complement(42310..42428)
                     /locus_tag="RHW33_001707"
                     /pseudo
     CDS             complement(42310..42428)
                     /locus_tag="RHW33_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10479 a        11063 c        10924 g         9962 t
ORIGIN      
        1 gggaaaaaaa cataaattta agcccaatcg aaaataatta aacttaatct cgtttaacct
       61 ttattgatat gtactacgta tcttatttac ttccggttta ctaaggaaac tgaatgcacc
      121 tgtaaaaatt acaggtttgg aaagtagtga catggcaaag tgattacagc agggactatg
      181 aggttaaaaa ccatatggaa tgtcaaaacc gttctgataa atacatctgg tctccccatg
      241 acgcctactt ctataaagga ctatctgaac tgattgtgga tatcgacaga ttaatttatc
      301 tatcgttgga gaaaattaga aaagatttcg tgtttatcaa tctcaatacg gattctttat
      361 ctgaatttat aaaccgtgat aatgaatggt tatccgcggt aaaggggaaa caggtcgtat
      421 tgattgcggc cagaaagtca gaagccttag caaattattg gtattacaac agcaatatta
      481 ggggcgtggt atacgctgga ctgagtcgtg atattagaaa agaactggcc tatgtgatta
      541 atggcaggtt cctgagaaaa gatattaaga aagataaaat cactgaccgg gaaatggaaa
      601 ttatccgcat gacggcccag ggaatgcaac ctaaatcgat tgccagaatt gaaaattgta
      661 gtgtgaagac agtgtatacc catcggcgta atgctgaggc caagctgtac tcaaaaatat
      721 ataagttggt tcagtaaact ccaggcaagt tagttttaaa aaatgactca ctgggacatc
      781 acgtcctcaa ttcaactcgg gaagaaatgc aatgaaaaaa aaggttctgg caatagctct
      841 ggtaacggtg tttaccggca tgggtgtggc gcaggctgct gacgtaacag ctcaggctgt
      901 agcgacctgg tcggcaacag ccaaaaaaga caccaccagt aagctggttg tgacgccact
      961 cggtagcctg gcgttccagt atgccgaagg cattaaaggt tttaactcac agaaaggtct
     1021 atttgacgtg gctatcgaag gtgactcaac ggctaccgcc tttaaactga cctcacgtct
     1081 tatcaccaac acattaaccc agttggatac ctcaggttcc acactgaatg tgggcgtgga
     1141 ttataacggc gcggcagtcg aaaaaactgg cgataccgtg atgatcgata ccgccaacgg
     1201 cgtactgggc ggcaacctta gcccgctggc taacggttac aatgccagca atcgtaccac
     1261 cgcacaggat ggtttcacct tcaccatcat cagcggcacc accaatggta ccaccgcagt
     1321 aaccgactac agcactctac cggaaggcat ctggagcggc gacgttagcg tacagttcga
     1381 cgcgacctgg accagttaat ctctctgatg taccagcagg ggtagccccc ctgctttatt
     1441 cctggacgga attgttatga aaaagcacct tctgcctctc gctctgctgt tttccggaat
     1501 atctccggcc caggcgctgg atgtcggcga tatatcatcg tttatgaaca gtgacagcag
     1561 cacgctgagc aaaacgatca aaaacagtac cgacagtggt cgccttatca atatccgtct
     1621 cgaacggctc tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca agccggatga
     1681 gttgctactc actcccgcca gcttgctgct acccgcccaa gccagcgaag tgatccgctt
     1741 cttctataag ggacccgcag atgaaaaaga gcgctactac cgcattgtct ggtttgatca
     1801 ggccctcagt gatgcgcagc gcgataatgc caaccgcagc gctgtggcca ctgcttccgc
     1861 ccgcatcggc accattctgg tcgtcgcccc tcgccaggcg aactaccact ttcagtacgc
     1921 caacggctcc ctgacaaata caggaaatgc gacgctgcgg atcctcgcct acggcccttg
     1981 cctgaaagcc gccaaaggta aggagtgtaa agagaattac tacctgatgc cgggcaagtc
     2041 gcgtcgtttt acccgcgtgg acactgcgga taacaaagga cgggttgcac tttggcaggg
     2101 tgataagttc attcccgtga aatagatagc tgtgcagatg gataacgaca atgcctttac
     2161 gacggttctc cccaggactg aaagcccagt ttgccttcgg catggtcttt ttgttcgttc
     2221 agcccgatgc cagcgctgct gacataagtg cgcagcaaat aggtggggtg attattccgc
     2281 aggccttcag tcaggcgctt caggacggca tgagcgtccc gctctatatt catctcgccg
     2341 gtagccaggg tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg
     2401 gacagctacg catccggaaa atacagctgg aagagagtga agataacgcc agtgtcagcg
     2461 aacaaactcg acagcagctg atggctctgg cgaacgcccc gttcaatgag gcccttacca
     2521 tccccctgac tgacaacgcg cagttggatc tcagcttgcg ccaactgctg ctgcagctgg
     2581 tggtcaagcg cgaagcgctg ggcaccgtac tacgctcacg tagcgaagac atcgggcagt
     2641 ccagtgttaa caccctcagc agtaatctga gctataactt gggcgtctat aacaaccagt
     2701 tgcgtaacgg cgggagcaac acatccagct atctgtcgct gaataacgtt actgcgctgc
     2761 gcgaacatca tgtggtgctc gacggctcgc tgtacgggat cggtagcggt caacaggaca
     2821 gtgaattata taaagcgatg tatgaacgcg attttgccgg tcaccgattt gccggtggaa
     2881 tgctcgacac ctggaacttg cagtccttag ggccgatgac cgccatttca gcagggaaga
     2941 tttacggcct ttcctgggga aaccaggcca gctccaccat cttcgacagc agccagtcag
     3001 ccacgccagt gatcgccttt ttaccggcgg cgggcgaagt acatctcacc cgtgatgggc
     3061 ggttactaag cgttcagaac ttcaccatgg gcaatcatga agtggatacc cggggtctac
     3121 cgtacggtat ttacgatgtg gaagttgagg tgatcgttaa cggtcgcgtg atcagcaaac
     3181 gcactcaacg ggtcaataag ctgtttagcc gggggcgcgg cgtcggtgca ccactggcgt
     3241 ggcagatatg gggcggtagc tttcatatgg atcgctggtc ggaaaacggg aaaaagacgc
     3301 gaccagctaa agagagttgg ctggcaggtg cctcgacctc cggctcattg agtacgctta
     3361 gctgggcggc aacgggatat ggatacgata atcaggcggt gggtgaaacc cgtctgacgc
     3421 tgccgcttgg gggggcaatc aacgttaacc tgcaaaatat gctggccagt gacagctcat
     3481 ggagcaacat cgccagcatc agcgccactc taccgggagg ctttagttcg ctgtgggtta
     3541 accaggaaaa aacccgcatt ggcaatcaat tgcgacgtag cgatgccgac aaccgtgcaa
     3601 tcggcggcac actcaacctg aactcactgt ggtcgaagct gggcacattc agcatcagct
     3661 acaatgatga ccgccgttac aacagccatt attacacggc agattactat caaaatgtct
     3721 acagcggtcc ctttggttcg cttggcctgc gggccggtat tcagcgctat aacaacggcg
     3781 acagcaacgc caatacaggg aaatatatcg ctctcgatct ctcgctacca ctgggcaact
     3841 ggtttagcgc agggatgact catcaaaacg gctacaccat ggcaaacctg tcagcacgca
     3901 agcagtttga tgaagggacc attcgcactg ttggtgccaa tctgtcacga gccatctccg
     3961 gcgataccgg tggtgacaaa actctcagtg gtggggcgta tgcacagttc gacgctcgct
     4021 acgccagcgg aacgctgaac gtcaatagcg cggcggacgg ctacgtcaat accaatttaa
     4081 ccgccaatgg cagcgtcggc tggcagggta aaaacattgc tgccagcggg cggactgatg
     4141 gcaacgctgg ggtgatattc aacaccgggc tggaggacga cggtcagatc agcgccaaaa
     4201 tcaacgggcg gattttcccg cttaacggca agcgtaacta tctcccgctc tctccctatg
     4261 gaagatatga ggtggagtta cagaacagca aaaactcact cgacagttac gatatcgtca
     4321 gcggtcgcaa aagtcatctg actctctatc caggcaatgt cgctgtcatt gagccagagg
     4381 tgaagcagat ggttaccgtc tccggtcgta tccgtgcgga agacggcaca ctgctggcta
     4441 acgcacggat taacaaccat atcggccgaa cccgaaccga tgaaaacggc gagtttgtca
     4501 tggacgtgga taagaaatac cccactatcg attttcgcta cagtggcaat aaaacctgcg
     4561 aagtggctct ggaactcaac caggcgcgcg gtgccgtctg ggtcggtgat gtggtctgca
     4621 gcggcctctc atcgtgggcg gcggtgacgc agacaggaga agagaatgag agttaaccta
     4681 ctaataacga tgataatttt cgcgctaatc tggccagtaa ctgcgctcag agcggcagtg
     4741 agcaaaacaa cctgggcgga tgcaccggca agcgagtttg tgtttgtcga aaacaactca
     4801 gacgacaact ttttcgtcac gcctggcggg gcgctggatc cgcgcctgac cggtgccaac
     4861 cgctggaccg gtttaaaata cactggctca ggaaccattt atcaacaaag cctcggctac
     4921 attgataacg gttacaacac cggcctctat accaactgga agtttgatat gtggctggaa
     4981 aattcgccgg tttcatctcc tttaactggc ttacgctgca tcaactggta cgcagggtgt
     5041 aatatgacca ccagtcttat cctgccgcaa accaccgacg ccagtggatt ttatggcgcg
     5101 acggtgacca gcggcggcgc gaaatggatg cacggcatgt tgtcagacgc gttttaccag
     5161 tatatgcaac aaatgcccgt cggcagcagc tttacaatga ccatcaatac ctgtcagacc
     5221 tctgtgaact atgacgccag cagcggcgcg cgttgtaagg atcaggcctc cggaaactgg
     5281 tatgttcgca atgtcaccca tacgaaagca gcaaatctgc ggttaataaa tacccactcg
     5341 ctggcggagg tatttatcaa cagcgacgga gtaccgactc tgggcgaagg gaacgccgac
     5401 tgccggacgc aaaccatcgg cagcctttca ggattaagtt gtaagatggt caactacacc
     5461 ctgcaaacaa acggactcag caacatctca atccatatat tcccggcgat cgccaactca
     5521 tcattagcct cggccgtcgg ggcgtacgat atgcagttca gtctgaatgg cagttcatgg
     5581 agaccagtga gcaataccgc ctattactac accttcaacg agatgaagag cgcagactcg
     5641 atctatgttt tcttctcgag caacttcttt aagcagatgg tgaacctcgg gatcagcgat
     5701 atcaacacca aagatctatt caactttcgc tttcagaaca ccacatcacc ggagtctggc
     5761 tggtatgaat tttctacctc caacacgctg attatcaaac cccgtgattt cagcatcagt
     5821 attatctccg atgaatatac tcagacaccg tcgcgggagg gatatgttgg cagcggcgag
     5881 tcggcactcg atttcggcta tatcgtaacc accagcggta aaacagctgc cgacgaagtg
     5941 ctgatcaagg tgaccggacc cgcgcaggtg attggcgggc gctcctattg tgtcttcagc
     6001 tccgatgacg gtaaggcgaa agtaccgttc ccggcgacac tttcctttat tacccgcaac
     6061 ggagctacaa aaacctacga tgccgggtgc gatgatagct ggcgggatat gaccgatgcg
     6121 ctgtggttga ccacaccgtg gactgatatc tctggcgaag tggggcagat ggataagacc
     6181 acagtcaaat tttcgattcc aatggataac gccatttcgc tgcgtacggt agatgataac
     6241 ggctggtttg gcgaagtcag cgcttcagga gaaattcatg ttcaggcgac gtggcgtaac
     6301 attaactaag gccctgctga cagcggtctg tatgctggcg gcacctttga cacaggcgat
     6361 ttcggtcggc aatctgacat tttcgctgcc gtccgagact gactttgtca gcaaacgtgt
     6421 agtgaataac aacaaaagcg cacggatata ccgtattgcc atcagtgcta ttgatagccc
     6481 gggcagcagt gaattgcgca cccgaccggt ggatggtgaa ctgcttttcg ccccccgcca
     6541 gctggcgttg caggctggtg agagcgagta ttttaaattt tactatcatg gtccacagga
     6601 taaccgcgag cgctactacc gggtctcatt tcgcgaggtc cccactcgta acctgacaaa
     6661 acgcagccct accggcggtg aggtcagcac ggagccggtg gtggtgatgg ataccattct
     6721 ggtagtacga ccacgtcagg ttcagtttaa atggtccttc gacaaggtga caggaacggt
     6781 aagtaacact ggcaacacat ggtttaagct actgattaag ccaggatgtg attcgaccga
     6841 agaggaaggc gatgcctggt atctacgtcc gggagacgtg gttcatcagc ctgagttacg
     6901 tcagccgggg aatcattatc tggtctataa cgacaaattc attaagatta gcgattcctg
     6961 tccggctaag cccccttcgg cggactgata cttatccatc ggtcgaggga agaattccac
     7021 ctcagagact caaaaaacgt ttaaatgatg tggaagcgat cgtcaatgtc acctgtaacg
     7081 atgggctttc tcatatgcac accatagtgt tcaggagaca cagaaaggta tcaggctgcc
     7141 tgctaacgag tggtttattc acgaaatagt acgtaaaatc ggtaacggct ggaaatcatt
     7201 caatactcac actatcgaaa gtacaccagc caaccgcggt acgttcttac atacgatgta
     7261 ccgctgttct ctttacgatt tatagctgta ctggtgaatt atgagcaatc tgaatccatg
     7321 catgacgtgt ggtgcctgtt gtgcattttt ccgcgtctct ttttactggg ccgaagccga
     7381 cgatgctggc ggtaccattc ccgccaggct cactgaacaa atatcccctt ttcaccgatg
     7441 catgagcggt accaatcaga aaaacccccg atgtattgcc cttgcaggaa ccccaggcaa
     7501 aaatgcctgc tgcacgatat ataaaaatcg atcgtccacg tgcagagaat tcgccatgtc
     7561 tggtgagaac ggagaagtca atgaggcttg caatcgtgca agggctaaat acgggctgac
     7621 accgttataa atatacaata attaattgca ctgccccgcc agcgataata gcggggcttc
     7681 gttttcaggg acaacaaaac ccgaaacttc ttgtatttgc cagcaacaat gcctctcttc
     7741 tgtagcgttc ttcggatcta catcatcctg aagtagcacg atatactcac tgactatctg
     7801 aatcagaata taaaaagcaa tgtttttaac ctataaaaat ggcgctgtat ttgcgccatt
     7861 tttatcattc aatgcattat ctgtttgagc ctaaagggat ctcagggtct ggctcatgag
     7921 taattctgtt tcgtaaatct ctgcgaataa tttcaatgga ccagaaccag actaaatgtc
     7981 caaaaatttc agaaacattc tcataccacg ggagatcaaa cagaggtggt gtcagtccca
     8041 tgagagggaa tgaaatcata tgaacaaaaa gttgggctaa agcacctgcc agtaagccct
     8101 gccagagttt aatttttgga aatacttcag cgaccacaca ataaccgaca gcaaacacta
     8161 tcgaaaagat aatgtgcgta acaccaaccc agttaaagac atgcccggca aaggtataaa
     8221 cagccgcatt gggatctgtc agccccagcc agtctcgaag aaaaatatac ggaggattga
     8281 gaaaattacg cgagcaatca atttgcccag cagccctgat taaggattcc gggccacacg
     8341 ctgcattaaa catatccacc gggctacgtg gcggcaatgg aacttcagcc cccacttcac
     8401 aaatgcggaa acaacgccag caatcagccc aatgaatgca gcaagaccat aacgtctgcg
     8461 gttaggtgga gtttgttcaa atatattcat atctaccctg cttgtaccat tatgttatac
     8521 acctctttag gagtattcat aaaacaaggt aaatgtaaag aactgtattg ttttgtataa
     8581 caagatggtt gtctaatcgc caaagaatat aagcaccatc atttctccct atttttatat
     8641 taaaagtgac atagatttgc agggtgatgc agagctgaca atcacaggtt tccttatcgg
     8701 tttttgcatc gtacaactca agcaataaac cagctcccat catcatcaac atcccggcga
     8761 acatgaattt attagccggg ctcatagctg cattcgcgtt ttaagaataa tcctcctgct
     8821 gtcgccgact atgcttaacg tttaaaaaag catcagcact ctcgcaacgc actcttattt
     8881 tcccctttag aataccggag gcctggtatg agcaaccaag gcgaataccc cgaagataat
     8941 cgggttggga agcacgagcc gcacgattta agtttgaccc gtcgcgatct gattaaagtg
     9001 agcgccgcaa cggcggcggc cgccgtggtt tatcctcatt ctacgcttgc ggcaagcgtt
     9061 ccggcagcta cacccgcgcc tgagataatg cccctgacac ttaaggtgaa cggcaaaacc
     9121 gagcagcttg aagtggatac ccgaaccacg ctactggacg ctttgcgtga aaatctgcat
     9181 ttgagcggta ccaagaaagg ttgcgatcac ggacagtgcg gagcctgtac cgtgctggtc
     9241 aatggtcgca ggcttaatgc ctgcctgacg cttgcagtca tgcatcaggg ggccgagatc
     9301 accaccattg aaggcctggg ttcaccagat aatcttcacc ccatgcaggc ggcctttatc
     9361 aagcatgatg gcttccagtg cggctactgc acctccgggc aaatttgctc atcagtagcg
     9421 gtgctaaaag agattcagga cggcattccc agtcacgtca cggtcgattt ggtttccgct
     9481 ccagaaacaa ctgccgatga gatccgtgaa cgtatgagcg gcaacatctg tcgctgtggt
     9541 gcatacgcta acatccttgc cgccattgaa gatgctgcgg gggagataaa atcatgaagg
     9601 cgtttaccta tgaacgagtg aacaccccgg ccgaggcggc acttagcgct cagcgcgtac
     9661 ccggcgcaaa atttattgcg ggcgggacca atctgctgga cctgatgaag ctggaaattg
     9721 aaacgcccac ccaccttatc gatgtgaacg ggctcgggct cgataagatt gaagtgaccg
     9781 acgcgggtgg gctgcgcatc ggcgcactgg tacggaacac cgacctggcg gctcacgagc
     9841 gcgtgcgtcg tgattacgcg gtactctccc gcgccctgct cgctggcgcg tctggtcagt
     9901 tacgcaatca ggcaaccacc gcaggtaatc tgctccagcg cacgcgctgc ccctattttt
     9961 acgacaccaa tcagccctgc aataagcgcc tgcccgggag cggctgcgcg gcgcttgaag
    10021 gctttagccg tcagcacgcg gtggtaggcg taagcgaagc ctgcattgcc acccatccga
    10081 gcgatatggc ggtcgcaatg cggttgctgg atgcggtggt ggaaaccatc acgccggagg
    10141 gaaagactcg cagtatcaca ctggctgatt tttatcaccc accggggaaa acgccgcaca
    10201 ttgaaaccgc cctgcttccc ggtgagctta tcattgcggt gacgttacct ccgccgctcg
    10261 gcggaaaaca tatctaccgt aaggtgcgcg atcgcgcctc ctacgccttt gccctggtat
    10321 cggtcgcggc gattattcag cctgacggca gcgggcgcgt cgcgctgggc ggagtagcac
    10381 ataagccctg gcgcattgag gctgcggatg ctcagctatc ccagggggcg caggccgtat
    10441 atgacgcgct gttcgccagc gcccatccca ccgctgaaaa cacctttaaa ctcctgttgg
    10501 cgaagcgaac gcttgcctcc gtactggctg aagcgagggc acaagcatga aatttgataa
    10561 acccgcaggg gaaaacccga tcgatcagct gaaggttgtc ggtcgtccct atgaccgcat
    10621 cgacggaccg ctgaaaacta ccggcacggc acgctacgcc tacgaatggc atgaagaatc
    10681 ccccaacgcc gcctatggct atatcgtcgg ttccgccatt gccaaaggac gcctcaccgc
    10741 ccttgatacg gacgccgcgc aaaaagcgcc gggcgtactg gctgtcatta ccgccagtaa
    10801 cgccggggca ctcggcaaag gcgacaaaaa caccgccagg ctgttaggcg gtcccactat
    10861 tgagcactat catcaggcca ttgcgctggt agtggcctag accttcgaac aggcgcgagc
    10921 ggcggcctcg ctggtgcagg cgcactatcg ccgtaataaa ggagcttact ccctggcgga
    10981 cgaaaaacag gccgtcagtc agccgccgga agacacgccc gacaaaaacg tcggtgactt
    11041 tgacggggca ttcacctccg ctgcggtgaa gattgatgct acctacacga ccccggacca
    11101 gagccatatg gcgatggagc cgcatgcctc gatggccgtc tgggatggaa ataagcttac
    11161 tctctggacc tcaaatcaga tgattgactg gtgccgcacc gatctggcaa aaacgctaaa
    11221 agtgcccgtg gagaatgtgc gtattatctc cccgtatatc ggcggagggt ttggcggcaa
    11281 gctcttcctg agaagcgatg cgctgctggc agccctcgcc gcccgagcgg tgaaacgtcc
    11341 ggttaaagtg atgctccccc gccccactat tcccaataac accacgcacc gccccgccac
    11401 ccttcagcac ttgcgtatcg gtgccgacca gagcgggaaa atcaccgcta tctcacatga
    11461 aagctggtcc ggaaacctgc ccggcggcac gccggaaacg gcggtacagc aaagcgaatt
    11521 actctacgcc ggggcaaacc gtcataccgg cctgcggctc gccacgcttg atttgccgga
    11581 agggaacgcc atgcgtgcgc ccggcgaagc ccccggtctg atggcgctcg aaatcgcgat
    11641 cgacgaactg gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac
    11701 tcaggttgac cccgccgacc cgacgcgccg cttctctcgc cgtcagctta tcgagtgctt
    11761 gcgcaccgga gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtacg
    11821 cgacggagag tggctagtcg gccacggcgt cgcggcgggc tttcgcaata atctgctgga
    11881 aaaatcgggg gctagggttc acctcgaacc aaacggcacc gttaccgtgg aaacggacat
    11941 gaccgacatt ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg
    12001 cgtaccgctg gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg
    12061 ttctggtgga caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtgtgaa
    12121 gcttcgcgaa atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga
    12181 cggcaagatt accaacggta cccgaagcgc cacgctaaat gaggccaccg caggcggcag
    12241 actgacagcg gaagagagca ttgaattcgg aacactgagc aaggagtacc agcagtcgac
    12301 ctttgccggg cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg
    12361 gcgtatgctc gctgtgtgtg ctgcaggacg catcctgaat ccgaaaactg cacgcagcca
    12421 ggtcattggc gcaatgacta tgggcatggg cgcggcactg atggaggagc tggcggtgga
    12481 tgaccgtttg ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc
    12541 ggatatccca aaacaggaag tgattttcct ggatgatacc gaccccatat cctccccgat
    12601 gaaggccaaa ggtgtcggtg agctgggcct gtgcggcgtg agcgcggcta tcgccaacgc
    12661 ggtgtataac gccaccggta ttcgggtacg cgattatccc atcactctgg ataagctgct
    12721 cgataagctg ccggatgtgg tttaaggagg aacaatgtca tacccgcttt ttgataaaga
    12781 cgaacactgg cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg
    12841 cttcgccgta gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat
    12901 acgcggcggc gcggcgcgcc cgctcggggc gcagatggtg gtgcgcgaag atggtcgtta
    12961 ctgcggtttt gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat
    13021 gatgggctca ggctgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat
    13081 cgttctgccc tgcggcggtg ggatcacgct gacgctccat aaactacgct cggcacagcc
    13141 tctgctcgcc gtgctgaacc gcctggaaca gagaaaaccg gcggggctgc gctacgatcc
    13201 gcaagcacaa tcgttggtgt gcctgcccac acaaacccga acgggctgga atctcaatgg
    13261 ctttgaggtg gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc
    13321 gcaggcaacc gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt
    13381 tccggcctca gccagcgctc agatcgatac cgatacggcg gtcattttgc tgtgccatga
    13441 tctcaaccgg gagctgccag tgttgcaggc cgcgcgagaa gcaaaaccct tttatctcgg
    13501 cgcattgggc agctatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg
    13561 gtccagggag gagacagcgc aaattcgggc acccgtcggg atattcccca aagcccggga
    13621 tgcgcatact ctggcactct ccgtgctggc agaagtcgcc tctgtacgtc tccatcagga
    13681 ggaggattca tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg aaagcgcttt
    13741 ctcacctccg ggggaaatac cataaattaa gagaaaaatt tgcagtagcc gttttagaaa
    13801 atcacctaaa tcgtttaaaa gagcgcccta cccatagtgg ttgctcatcg ccttgtgtaa
    13861 ctttttcagc aacgcgcgcc ctcgccggac taaagcgcac cggccttctg gcgtgctgta
    13921 acaaaggtca gtacgcccgc gcaaaggagc agcacactac tcatggcaaa cgtactctgc
    13981 cagccaaggc ggtcaaacac gatgcctcct gccgttgatc caagggcgat tgagagctga
    14041 atcaccgcga ccatgagccc gcctccggct tcggcgttgt caggcaacgt acgcgcaatc
    14101 catgtccacc atccggttgg cgcggcggtg gccagcatgc cccagagtcc taacagcagc
    14161 gagaccgtcc agacgtgatg cccggtaagc atcagaccga tggcaatgac ggccatcagt
    14221 ccgggtatgg ccatcagcgt ggggtagaat tttctgttga gaaatgtcga aacaaccagc
    14281 gtgccaataa aaccggccac gccgataatc agcaaaatca acgacaagcc caaggaatta
    14341 acccgtgtca ccgactccag aaacgggcgc acataggtga atagcgcaaa ctgccccatg
    14401 aaaaacaggc cacaagctat catgccgacc acgaccatgc ggcggctaaa taagcggaat
    14461 accgcgccaa atgacgcgtg gcttttattc gcatccatgc tgggcagact aatacactgc
    14521 cagataaagg cgacaaccgc gataggcacc aggcacaaga aagcgcctcg ccatccgacc
    14581 gtagccccca gatagcttcc cagcggtgcg gccacgaccg ttgccagcgc attaccggcg
    14641 ttgaaaatcg ccagcgcacg cgtgacctga tgctgcggca ccagacgaat cgccgttgcc
    14701 gctgacatcg accagaatcc gccaatggcc acaccaatca tcgcacgacc gaccatatac
    14761 atcagataat tggaagccag cgcaataata agccccgata cggccatcag aaccgtcatc
    14821 tcaagcagca ggaacttacg attcatcttt ccagctagcg ttgaaagcgt caggctggtc
    14881 agaaccgcca gcgcgccgga gatagcaatc ccctgccctg caagcccctc cgtcacgcct
    14941 aaatcgcggg taatcggcgt gagcaggctg acgggcataa attcagaagc aatcagcaca
    15001 aacacgcaca gggtcatggc aaaaataccg ccccagtatg cgcgctgatg atgaggtgat
    15061 ggtgtttggc ttagtgtcga catagtgaaa tttcacaaag atcaggataa aagccacctg
    15121 ccatagaccg gtggctttgc atcaattcag gagaggtttt atttcagttt ggtctggaaa
    15181 aattgttcga acttcgcgaa aggtattttt ccggccacgt tatcgtacag gtccacgtga
    15241 tttgctcctg gtaccaccac cagctctttg tcgttactgc caaccacctt gtaagcatct
    15301 tcagcaaaat agcgtgaatg cgctttctca ccggtcacaa tcagcgtcgg gatggtgatt
    15361 tcactggcgt agctcaacag cggcatattc ataaatgaca gcggcatcgt tgccgtccac
    15421 gccccggtag agttaacgga acgttcgtgg aagccgcgcg gcatgcgata gtagtcaaag
    15481 aactctttca gtaccggatg aggatttgcc ggcagcgttt ctggcagaat acgctcgccc
    15541 gccctgacct tgccgttgct atcgacatta atatcatgac caccgtgagc gaacgttccg
    15601 ctctgcgcat ccttccagcg ctgttcattc agatattgca gaacagcacg acggtcggcg
    15661 gtagaataac ggtcttcgcc atcccccaca ccatggccca tcgcccggct catgtcatac
    15721 atcacgctgg tagcaaccgc tttaacgcgg gtatccatcg ccgcgtcgtt cagcgccatg
    15781 ccgccccagc cacaaatacc gagcagcccg atacggttgc gatctacctc tttttgcagc
    15841 cctaagaaat ccaccgccgc gctgaaatct tcagtgttga tatccggtga ggcgacgttt
    15901 cgcggatagc cgccgctttc cccggtgtaa gaaggatcga acgccagggt aacaaaccct
    15961 cgttccgcca gcgtctgcgc atacaagccg ctggattgct ctttcaccgc gccaaatggc
    16021 ccactgaccg caatcgccgc cagcttgcga tcgccacggt ctttaggcag gtaaagatcg
    16081 cccactaagg tgatgccata tcggttcggg aacgatactt tacggtgatc gactttctgg
    16141 ctctcggcga acgttttatc ccatttatct gtcatggaca caggggcatt aggattggtt
    16201 gaatcagcat aactcattgt cgtgactcca cttaatgatg cgaaaagcag catggccggc
    16261 atcgcggttt ttagcgttcg ggtgaaggct ttcataaaaa tccccataaa aaacaacaga
    16321 gagatacagg tttccgacgg acaacgctgc ggtgctttac tttgatgggg tcattatagt
    16381 gagcagaatt agtgcagatt agggggcgat taatgctaag attaatacca tattgttata
    16441 aattaatgcg aagattgccg gcatgaaggg gggatgtttc aatggcgaaa agggagaact
    16501 acaacgagct gtacctgttt atgcaggtgg tgcgagaagg cagctttacc gcagcggctc
    16561 agcggctggg tcttgctcag tcaggagtca gccgttctgt tcgcgagctt gaagaaaggc
    16621 tgggtgtcca gctgctggtg cgcaccacgc gtaaactgtc gctgacacaa gcaggcgagc
    16681 agctctacca gaagacggca tctggatttg aaatgttaga tcttgggctt gccacgctgg
    16741 cgcactatcg agagacgccc tccggcacgg tgcgtatcaa tgccagccag cacgcgattg
    16801 ataaatgcct gctgccaaag ctggcggtat ttaaacagcg atatcctgat atccggcttg
    16861 aactgataaa tgagagccgg ttcgtcgata tcatcgagga aagattcgat gcaggtgtcc
    16921 gcctggggcc tgaagtcggc cagggcatgg tcgcggtacg catcacgccc gatatggaga
    16981 tggcaattgt ggggactccg gaacattttc gtcgctatgg ttttccgcaa accccggcgg
    17041 atttaaaggc gcatccctgt atcgcctatc agtttgccga cggcagcgta taccagtggg
    17101 aactcaatca ggatgataaa aaaatcactc atcgccctga agggcaatgg gccctatccg
    17161 acagctatat ggaagcagaa gccgcccggc tgggcctcgg attggcctat gttcctgttg
    17221 aactggtcgc ggatgattta gaacacggta agcttatcag ggtgttgcag cgttacagcc
    17281 tgcgaatgga gggattgttt ctctattacc ctcatcgcaa cgtctccccc gctctgcgga
    17341 tggtcattga tacattgaaa atctgaacgt taacaacgac cgactcttca ctgtgcaata
    17401 ttgtgtcagg tagtcactct gcgttcaatt ccaggggccg ctttgagcga gaaagagcgg
    17461 acttatctgt acaaggggac tatccagcaa tggagatcat ccaaaagaaa aaaccacctg
    17521 gctcccctcg acaaccttaa tccgggcatt tagcctcaat caacattttc atatctgtca
    17581 ggttacttca ttatgaaaac cgatcaatat cgttgttttt gttgcaacag tagaatacgc
    17641 gtatttatcc ctccatttct attaataacg gcaataaact gttcatttca ctgatattta
    17701 aaatatgcat ccttttcaag ctgttcagcg gcataagaaa gcgtattaaa tatgaattcg
    17761 acgatttaac catttctgtc tgattggtaa tcatctgtga ttaatgctgg tctattgatt
    17821 acgccagcat taaccgctta gggatgtatt cttcctgatc atatacagcc ctcgaaaaag
    17881 agagaaaata aaactgtgtc caatattact ctaccactcc agaccgaagc atcggtagaa
    17941 ttagcagaac gactggtaca gaaggtgggt atagcgtcat ttgtcgggca ttccattgcc
    18001 atcataatga taattttgaa tcaatagacg tgcttcttga tcatgtctta aaattatacc
    18061 atgagcctta tgaagcatca tttttactga ctggtgttaa ctttgctatc atataaactc
    18121 tgttcttaaa aaacgcgccc agaattttat atgatttccc ttgttttctg cctgtaaaga
    18181 aatttttttc agtgctgtta atggttgaac cgacggcagt aatcgttttg gcgaggctac
    18241 tgaatgataa taaagacagt gcaaccacaa caattaaaga acagatagta ttcatgttaa
    18301 ttctcctgac aattgagagg atgagtttta tatacatagg tattaaacat gagtcatatt
    18361 taccccaaga gaaaacactc cttctgtaat taaaccctca tctccttcgg ggtgagcaat
    18421 acataatgca tcttgctatg caaaacaggt tgatataaat tttgcttctg acatatttaa
    18481 taaaacgtga atgtaaaaat tattcttcag tttgcagatg cagtgggtct gatggtaatt
    18541 tcggttgtat caacgctttc gggagattca ataatatgcc ttaccgcacg ggcaatatcc
    18601 gcaggttgaa gtgcaacaga acggtaggcg tccattgcct tcatggtttc cacatgggta
    18661 atggttgaag ccagttcact ttcgacaacg ccaggattta cgcaggtcac acggattttc
    18721 gagctttcct gccttaagcc atcagaaata gcccgtactg caaattttga tgcacaatag
    18781 actgcgcctg tgggcacaac agaaagggca ccaatagaac caagattgat tatctgaccg
    18841 gaaccctgtg cttccatcac cggaagtaca gcgccaattc cccacagtac acctttgata
    18901 ttcacgtcaa tagtgagctc ccattcattc tgttttcctg ctgcaagcgg tgaaagcggc
    18961 ataacgcccg cattattgat aagaacatca actcgccccc agctatccag cgctacttgc
    19021 acgaaatctg ccatggactg tcggtctgtg acatccaact cccgagcttt agcaattcct
    19081 cctgcgcggc aaatttcggt tgcaatggct tcgatacgct caactctgcg tgctcccagt
    19141 aaagtctttg cacctgtcgt tcccagctct ctagcaatac cttccccaat accacttgat
    19201 gcgccggtga ttaatatgac cttatccatt acgttctcct tcaaatgtgt tcggagagag
    19261 aatagaactt tatgattggt tccagtatcc tgctttcaat gtactcagtg gttagcggca
    19321 ttaaacaatg aaaatcgatc ttaatctttt acccattttt atcgcggtag cagaagagag
    19381 taatttcagt aaagccgccg cacgactggg cgttacccgt tcagcggtca gtcagggaat
    19441 aagacgtctt gaagatgcat tcgggacaat gcttgtaatg cggaccacgc gctcagttaa
    19501 tttgacggaa gcgggagaac ggctgcataa atcgttgtca ttacctttat ccggtattga
    19561 ggcagcgttc gaagagatgg tctctgacca tgtaccacgt ggacatctca gaatcgcggt
    19621 gacgtcgata gcagaagaat ttctttcagg tccgctaatc gcttcgtttg cagctgccaa
    19681 tccagccgtt acgcttgata ttgttgtcac ggatgaggaa ttcgatatcg ttgccgcagg
    19741 ttacgatgct ggcgtaaggc tcggagaggt cattgaaaaa gacatgattg ctgttcccct
    19801 taccggtaag cagcgtgaaa tggtcgttgc ctctccctcc tatctcgcag ccaacagcac
    19861 acctgttcat ccgcgtgaac ttgtgaatca taagtgtatc ggctggcgtc aatccccgga
    19921 tgttgcgcct tatcgctggc catttgaaga aaacgggaga gctttcgacc ttgcgattga
    19981 accacagatt acgactaatg atctgcgtct gatgttgagg ctggctcttg ccggcggagg
    20041 aataacaatt gccactcagg aaactttcag gccgtatatt gaaagcggta agcttgtatc
    20101 tctgcttgat gactttcttc cacaatttcc gggcttctat ctgtatttcc cacagcgtcg
    20161 caatattgca ccaaagctcc gcgccctgat tgaccacgtc aaggaatggc ggcagcaatt
    20221 ggcttaaatg tctgcacctg cattgcctga tgtcagaaca gtattttgat taattgccag
    20281 ggttacaatg gcataaatac ggcacaggag gaaacgtggt gtatttgaat atggggtaac
    20341 tacttgattt aaatggtgcc gataatagga gtcgaaccta cgaccttcgc attacgaatg
    20401 cgctgctcta ccaactgagc tatatcggcc ctgaaaggac atgttcacga acgtgaatca
    20461 cggtggacaa ggttaaaact aaccgggcga tgcgtcaatg gccttgtgaa tcaaatggct
    20521 acttttgcat cacccggttt tatttacgca cgaatggtgt aatcaccaat gccgatccac
    20581 ttgtaagtgg tcagcgcttc cagccccatt gggccacgcg cgtggagttt ttgtgtgctt
    20641 accgccactt ccgcacccag tccaaactgg ccgccgtcgg taaaacgcgt agaggcgtta
    20701 acgtaaacag cggacgaatc cacttcgtta acaaaacgct gggcgttgcg catatcgcgg
    20761 gtcaggatcg catcggagtg ttgtgtgccg tgttcacgaa tatgggcgat ggcatcgtca
    20821 agatcgctga cgattttgac gttcaaatct aatgacagaa actcatcgtc atactcttcg
    20881 gctttaaccg ccaccacctt cgcggggcct gcctgcaact gtgtcagcgc tgctgcatct
    20941 gcgtgtaatg tcacgccgct ttccgccatt tgtttgctta atgcgggcag gaagctatcg
    21001 gcgatgtttt tattcaccag caacgtttct accgtattac atgtgctcgg acgctgagtt
    21061 ttcgcgttga cgatcacttt taatgcttca gcgatctctg cactttcatc aacgtaaata
    21121 tggcatacgc ctataccacc tgtgatcacc gggatcgtcg actgttcgcg gcacagttta
    21181 tgcaaaccag ccccgccgcg cgggatcagc atgtcgatgt atttatccat acgcagcatt
    21241 tcactgacca gcgcacggtc aggattatca atcgcctgca cggcacccgc cggtaagcca
    21301 caggatttca gggcgtcctg aatcaccgcc accgttgccg cgttagtgcg acaggtttct
    21361 ttgccaccac gcaggatcac cgcattaccg gttttcaggc acagtgaagc aacatcaacc
    21421 gtcacgttcg ggcgcgcttc ataaatcacg ccaataaccc ccagcggtac gcgacgacgc
    21481 tcaagacgca ggccgctgtc cagtacgccg ccatcgatta cctgccccac cggatcggcg
    21541 aggttacaca cctggcgcac atcgtcggca atgcctttca gccgtgcggg cgtcagtgcc
    21601 agacggtcaa gcatcgcttc gctaaggcca ttggctcgcg cgtcagcaac atcctgggcg
    21661 ttagcgttga ggatgatttc gctttgtgct tccagttcat cggcgatttt ttccagcacg
    21721 cgattttttt cgcggcagga gagttgcgct aatttatacg aggcttgctt cgcggcaatg
    21781 cccatttgtt ccagcatcgg ccagctcctt aacgggtaat catgtcatca cggtgaacgg
    21841 caaccgggcc gtattcatat cccagtattg catcaatttc ttgcgagtgg tgtccggcaa
    21901 tacggcgtaa tgcatcgctg ttgtaacgac tgacgccgtg ggcgatatcg cgaccttcga
    21961 ggttacaaat gcggatgact tcaccacgcg agaagttgcc agtcacgctt ttaatgcctt
    22021 tcggcaacag ggagctgccg cgttcaagaa tggcggcggt tgccccttca tctaccgtga
    22081 tttcacccgc cggcggcgca ccgaaaatcc agcgtttacg gttttcaagc ggagtcgcct
    22141 gggcatggaa cagcgtaccg acggaaatgc cttccatcac atcaccaatg acgcccggct
    22201 tgctgcccgc ggcaataatg gtgtcgatac ccgcacggca agccacgtcc gcggcctgca
    22261 atttggtact catgccgcca gttccgaggc ctgaaacgct gtcaccggca atcgcgcgca
    22321 gtgcgtcatc aataccgtaa acatctttta tcaattctgc ctgcggatta ctgcgcgggt
    22381 cagcggtata caaacctttt tgatcggtca gtaacaacag tttatcggca cccgccagaa
    22441 tcgccgccag tgcagaaagg ttgtcgttat cgccgacttt aatctctgcc gtagcgacag
    22501 catcgttctc attgattacc ggaacgatat tgttatcgag caacgcacgc agggtgtcgc
    22561 gggcgttcag gaagcgttca cggtcttcca tatcagcacg ggtcagcagc atttgcccga
    22621 cgtgaatgcc ataaatcgaa aacagctgtt cccacagttg aatcagtcga ctctgcccta
    22681 ccgccgccag cagttgtttc gaggcgatag tcgctggcag ttccgggtaa cccaggtgct
    22741 cacgtccggc ggcgatcgcg cccgacgtca caataacaat ccgatgcccg gcggcatgta
    22801 actgcgcgca ctggcgaaca agttcaacga tatgggcacg gttcaggcgg cgcgatccgc
    22861 ctgttagcac actggtgccg agttttacca ccagcgtctg gctgtcactc atgattctct
    22921 gccattcaat tttaggaaaa atgatatcaa acgaacgttt tagcaggact gtcgtcggtt
    22981 gccaaccatc tgcgagcaaa gcatggcgtt ttgttgcgcg ggatcagcaa gcctagcggc
    23041 agttgtttac gcttttatta cagatttaat aaattaccac attttaagaa tatcattaat
    23101 ctgtaatata tctttaacaa tctcaggtta aaaactttcc tgttttcaac gggctctccc
    23161 gcagaatatt cgcgcgttaa ttaaaatcag gaatgaaaat gaaaaagagc actctggcat
    23221 tagtggtgat gggcattgtg gcatctgcat ccgtacaggc cgcagaaata tataacaaag
    23281 acggtaataa actggatgtc tatggcaaag ttaaagccat gcattatatg agtgataacg
    23341 acagtaaaga tggcgaccag agttatatcc gttttggttt taaaggcgaa acacaaatta
    23401 acgatcaact gactggttat ggtcgttggg aagcagagtt tgccggtaat aaagcagaga
    23461 gtgatactgc acagcaaaaa acgcgtctcg cttttgccgg gttgaaatat aaagatttgg
    23521 gttctttcga ttatggtcgt aacctgggcg cgttgtatga cgtggaagcc tggaccgata
    23581 tgttcccgga atttggtggc gactcctcgg cgcagaccga caactttatg accaaacgcg
    23641 ccagcggtct ggcgacgtat cggaacaccg acttcttcgg cgttatcgat ggcctgaact
    23701 taaccctgca atatcaaggg aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg
    23761 gcttcggcac gtcattgaca tatgattttg gcggcagcga tttcgccatt agtggggcct
    23821 ataccaactc agatcgcacc aacgagcaga acctgcaaag ccgtggcaca ggcaagcgtg
    23881 cagaagcatg ggctacaggt ctgaaatacg atgccaataa tatttatctg gcaacttttt
    23941 attctgaaac acgcaaaatg acgccaataa ctggcggctt tgccaataag acacagaact
    24001 ttgaagcggt cgctcaatac cagtttgact ttggtctgcg tccatcgctg ggttatgtct
    24061 tatcgaaagg gaaagatatt gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg
    24121 tcggggctac atattatttc aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc
    24181 aactggatag cgataacaaa ttgaatatta ataatgatga tattgtcgcg gttggcatga
    24241 cctatcagtt ttaatgatta ttgccggatg tgatgcatcc ggcacatttc actcacgccg
    24301 ttaacttcac cggctcgtca cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg
    24361 tcagcagttc acgcagcttc tcgtgaaact cacgcagggt gtgctccagt cgttcaacca
    24421 cttcagtgtc ttttaccgga acgctcttcc agtcgcctgc tttatcgaac agaccaaact
    24481 ggtaactgta ggtaaaacgg gattcctgcg cttcaagctc catccaccag ccccagaact
    24541 cacgcacttc cggtgccggt ttcacgttga cgcatacagc cagacaatcg aaaaagaatc
    24601 gattatcttc acacttacct tcacgaatat acgggcctag tgcggtaaat tttttgatca
    24661 atctgctctt cgggtgtcca ctcggtaacg tcattgctat ctcctgttgt gatgcaactg
    24721 ttttaccaaa ttggcaaaat ttagcaaatt tttaacacaa gcgtttttcg atccagtcgg
    24781 tgatttcctg aagacctttg tcaaaattcc gatacaccgg gttaaatggg atctctaata
    24841 atttaccgtc agcagatgat gaggtgatta agcgtgagtc ctcttccggg ctgaacggat
    24901 cgttcttcca gtagcctgat aacattggcg ttgggcagcg acgtcccagc aatccttgca
    24961 cttttaatga atagcgattc agctccacgc gcaacgcttc gtcggaagca tcatgcatcc
    25021 ccaaacgact cgccagaacg tcaagataca tttccggcac ctgttgctgg cacttaaaat
    25081 cactcaacag ggtatgaact accggaccaa gacaggcaac cgctttcaga cgcggcgatt
    25141 caagatatgc cagacgcacg gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc
    25201 gagtgtgatc cacccacggt acgttaggca gcgcctttaa gacgtgctga tgcaacaggc
    25261 tggagtcctg ggtgagcttc cattttgatg aaaagcccac cgacggcata tcaatagtca
    25321 gcatcgcaat gccgcgcggc gcaaaataac gttcatacag gctgtaatag tccgtctgca
    25381 tcgcatccag accaccacac attaatactg tcgggaacgg gccatcgcct ttcggcatat
    25441 gcaaaaagcc ggtgatgggc gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc
    25501 ccggcagacg ctgagcggcc tcttcatagg cgcggtttga caaagcctgc gcttgctcgg
    25561 ccaggtcatc tcctttcaga tgaggatagg cggcaatgtt gtacaacgta gccgcatgca
    25621 gccagtgacg accactgagt tgcggatctt cttccgcaca ggctttttgt tgccacacca
    25681 tcgcctgggt ggcccactca taaatccagt tgccgccacg gtagccaatc accgtatcgt
    25741 ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat acgtgcctgg acgtcgagga
    25801 tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg gttaatcatg cgataccagt
    25861 gagggatggt tttaccatca agggccgact gcacaggcgg ttgtgcgccg tgattaaagc
    25921 ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg tttgaacagg gtttcgctca
    25981 ggtttgcctg tgtcatggat gcagcctcca gaatacttac tggaaactat tgtaacccgc
    26041 ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt gaaaagatta gcgaccggag
    26101 attggcggga cgaatacgac gcccatatcc cacggctgtt cgatccaggt atcttgcggg
    26161 atatcaacaa cataatcatc aaccagcgga cgaccagccg gttttgcaaa gatggtgaca
    26221 aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag taccaccggt atctaccagg
    26281 tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg
    26341 cgctggttgt cgtgatcgta gctggaaata caaacggtat cgacatgacg aatacccagt
    26401 tcacgcgcca gtaacgcacc cggtaccaga ccgccacggc ttacggcaat aatgcctttc
    26461 cattgttcag aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc
    26521 caggtgacga tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa
    26581 aaagtgttcg aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc
    26641 agtatttcac atgagtccgc gtctttttac gtactgcctc tccctgacgc gggataaagt
    26701 ggtattctca aacatatctc gcaagcctgt cttgtgttga caacattttc tgctaaccct
    26761 gtgacctgca atactgtttt gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc
    26821 tcaattatct ccacagccgc tgtgggatat ttttgccaaa atctgttcta ttcctcaccc
    26881 gtcctatcat gaagagcaac tcgctgaata cattgttggt tgggcaaaag agaaaggttt
    26941 ccatgtcgaa cgcgatcagg taggtaatat cctgattcgt aaacctgcta cagcaggtat
    27001 ggaaaatcgt aaaccggtcg tcttgcaggc ccacctcgat atggtgccgc agaaaaataa
    27061 cgacaccgtg catgacttca cgaaagatcc tatccagcca tatattgatg gcgaatgggt
    27121 taaagcgcgc ggcaccacgc tgggtgcgga taacggcatt ggtatggcct ctgcactggc
    27181 ggttctggct gacgaaaacg tggttcacgg tccgctggaa gtgctgctga ccatgaccga
    27241 agaagccggt atggacggtg cttttggctt acagagcaac tggttgcagg ctgatattct
    27301 gattaacacc gactccgaag aagaaggtga aatctacatg ggttgtgcgg ggggtatcga
    27361 cttcacctcc aacctgcatt tagatcgtga agcggttcca gctggttttg aaaccttcaa
    27421 gttaacctta aaaggtctga aaggcggtca ctccggcggt gaaatccacg ttggcctggg
    27481 taatgccaac aaactgctgg tgcgcttcct ggcgggtcat gcagaagaac tggatctgcg
    27541 tcttattgat ttcaacggcg gcacactgcg taacgccatc ccgcgtgaag cctttgcgac
    27601 cattgctgtc gcagctgata aagtcgacgc cctgaaatct ctggtgaata cctatcagga
    27661 gatcctgaaa aacgagctgg cagagaaaga gaaaaatctg gccttgttgc tggactctgt
    27721 agcgaacgat aaagctgccc tgattgcgaa atctcgcgat acctttattc gtctgctgaa
    27781 cgccaccccg aacggtgtga tccgcaactc cgacgtggca aaaggtgtgg tcgaaacctc
    27841 cctgaacgtc ggtgtggtga ccatgactga caataacgta gaaattcact gtctgatccg
    27901 ttctctgatc gacagcggta aagactacgt ggtgagcatg ctggattcgc tgggtaaact
    27961 ggctggcgcg aaaaccgaag cgaaaggcgc atatcctggc tggcagccgg acgctaattc
    28021 tccggtgatg catctggtac gtgaaaccta tcagcgcctg ttcaacaaga cgccgaacat
    28081 ccagattatc cacgcgggcc tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga
    28141 catggtttct atcgggccaa ctatcaccgg tccacactct ccggatgagc aagttcacat
    28201 cgaaagcgta ggtcattact ggacactgct gactgaactg ctgaaagaaa ttccgacgaa
    28261 gtaattattt gatttgctgc cggatggcgt tcaatcacct tccggcagtt tcatccttca
    28321 ttatccttcg ataaaagcca tccctgtaaa tgtccgtcgc gggttgccac gttcaatctg
    28381 gtgatggatc attcgccgct gtgatttcag cgccgcgcta ttttcctgtt gctgctgctc
    28441 cagcctccag gcaatcagca atcgcgccag ccgtttgttg gcatgctggc tgcgctctga
    28501 ctgaaccttc acgctaattc cggtagccag atgcgtggcg cgtaccgccg agtcggtttt
    28561 attgacatgt tgaccgcccg gccccgacga acgcagcgtc tcataacgga ccgcgtcgga
    28621 ttgctcctgc tcatcagcag taaaacgccc aatgcccaga aaccagtttt tgcgtccatg
    28681 atgaggccga tacggactcg gacaaatcca ctgaatagtg ccgcaccaac tttcgcttaa
    28741 cgcccatgcg ttgtcgccat ccagagaaat cagcgccgaa cgcagcgtgt cagagtagcg
    28801 gcccgtttct gtttccagca ccgttaccgc gacgtcttgt cgggtaaaaa cgaccaacag
    28861 agaagaacgc tgcgccaatc gggtagccgc gtccggggtg taaatctggc atccccacta
    28921 cgcgctgcat gttgggtaaa tttgccgtgg tgtgtaattg ttggatcgct aaactctcga
    28981 tccacaggtc atcagatgcg atggtaaata ccgcatcgga taagggacga atatatttgc
    29041 ccatgtacca ttccatttat actcgtcata cttcaagttg catgaactgc gactcgaatt
    29101 atttagagta tataaatata aaaatcagga aatggcagac gaatccaggg caagaaaaaa
    29161 agaattaatc ctggaaagat ctgcaaagag tcattgataa aggatttgtc atgatttacc
    29221 tccttgcagt ttgtggaatc agaaaacgcg cacataccag gcgggcgtta ttttcattgc
    29281 aagctggatt taatgttgcg gtttatatcg catataaaaa ttagtaaacc acgctccccg
    29341 gcattcaacg cgctgctgct taactgtctg aaaaccataa cgttcaaaaa aaggttttgc
    29401 ggttatgctt gcgtccactg taagttcgga ttcagactta atcaaaggtt ttaacaaagc
    29461 actggcaacc ccacggcggg tgtattcagg gtcaacaaat aacatatcga tataatgttc
    29521 aatgcgggaa ataaaaccaa ccggttgtgc attaatgact gcaacccgca cttgtgattt
    29581 cgcgagtttc tccttccagc gagattcgtc aatctgcgcc cagacggcaa tttgttgtgg
    29641 tgaataatgc tcactggctg tcatcgtaac cgctctaatg aaaatagcgc atagttgctg
    29701 aaaatcgcca ggctgatagt ttcttatttg tatgttattc ataatcccag caccagttgt
    29761 ctttccattt gagggtcaag caacgtcaca tgcagcccca ccagtcgcac accgcgcccg
    29821 ccgcggcgtt catcccaggt tttacgcgcg gtggcgatta agtcagcttt attcagccgc
    29881 ggccagacgt gctcctgggt ggtttgctga aaatcgtcga actttaattt caccccctgg
    29941 cgagcaatca gtaaatcagg ttttaccttt gccagacggc gttcaagttc cggatacagc
    30001 cgctcgataa tcgcttcaca ttcagaccag tgatgaatat cttccgccat cgtgcgttcc
    30061 acgccgacgg attttcgtaa ccgttcgctg ttaacgtcgc gctcgtcaat cccctgacta
    30121 cgctcccaca aaatgcggcc aaatttgcca aagcgtttaa gcagaatcac cagatcacac
    30181 ttttgtacat caccgcaggt ccgcagcccc atcgcttcca gttttgccgc cgagactttg
    30241 ccgacgccgg ggatttttgc cagcggtaag gtttgcagaa atgccggaac ttctgccgga
    30301 gtaatcacaa actggccgtt gggtttattc atgtcggagg cgattttggc gagaaacttt
    30361 accggcgcaa cgcccgcaga cgccgtcagt tgcagctcgt tgaaaatcgt ctggcggatt
    30421 tcctgggcaa tgagggtcgc agaaccgtgg caatggacgc tatcagtgac atcgagatag
    30481 gcttcatcca gtgacaacgg ttcaatacgc gaggtgtagc gcgagaagat ttcacggata
    30541 tgatttgagg cttctttgta ggcgtcaaag cgccccggaa gcaaggtgag atgtgggcat
    30601 aatttgagcg ccatccctgt cggcatagcg ctacgtacgc caaatttacg cgcgggataa
    30661 ttggcggtgc tgatcacccc ccgacgttcg cggctaccgc caatagcaat agggatatcg
    30721 cgcagggcgg gattgtcgcg catctccacc gctgcgaaaa agcagtccat atccacatga
    30781 atgattttac gcattgctca cctctcaaca ctggtaaagt atacagtgat ttcagggttt
    30841 gagaaatgcg taaagattca gcatttagcg cattcgctgc gaaagcacct gctgcttatc
    30901 cagcttttgc accagcggct gcaccacttt atccccatgc tgaccaaagt attgatacag
    30961 cgtatcggac gcacttttgg tcagcaccat aatctcaata cgccgattgc ccgcgctttg
    31021 cggatttttg gcatccagca gcatctggtc cgccattgcg cttacctgca tcactttatc
    31081 ttccggcatt ccggcctctt ccagcacccg acgagctgaa agcgcgcgat cgcccgaaag
    31141 gttccagttg ttgtagatat tgtttttgta ggccatcgca tcggtatgcc cggtaataat
    31201 aattttgtta tcgagcgagt cgaacactgg cgcgagctcc accagcaaag ttttaaagaa
    31261 cggcataatc tgcgcgctgc cgcgttcgaa catattgcga ttctggtcgt ctttaatcag
    31321 cacgcgtaat ccctgcggga caatctccat ctccagattc gcttccatat gcgcatcacg
    31381 ggcaatggtg ttgatactgg ttgccagctc gccaagctcg gtggcggatt tcttcttcag
    31441 cagcgccgtt ttctcattca catcacgcgc ttttttctcc gtctcttccg gcaccgccac
    31501 ggtggcaggt tttggcggat gagacgggct gattttattt aacggcgaca gcccaccacc
    31561 gttgaaaatc gactgcccgt gcagcgcggc gataatgctt tcacgctcag atttactgac
    31621 gctgttgaca atcaacttta tcgtcgtcac caaaggggcc gagcgtattt ccgaggtttc
    31681 tgcccgcttt accctggacg cgatgcccgg caagcagatg gcgattgacg ccgatcttaa
    31741 cgctgggttg atcaaccagg cgcaggcgca aacccggcgt aaagatgtcg ccagcgaggc
    31801 cgatttctac ggcgcgatgg acggggcatc aaagtttgtg cgcggggatg ccatcgccgg
    31861 gatgatgatt ctggcgatca acctgatcgg cggcgtctgt atcgggatct ttaaatacaa
    31921 cctgagcgcc gacgccgcct tccagcaata tgtgctgatg accatcggcg acgggctggt
    31981 ggcgcagatc ccttccctgc tgctctccac cgcggcggcg attatcgtca cccgcgtcag
    32041 cgacaacggc gatatcgccc atgacgtgcg ccaccagctg ctggcaagcc cgtcggtgct
    32101 ctacaccgct accgggatca tgttcgtgct ggcggtggta ccggggatgc cgcacctgcc
    32161 gtttttgctg ttcagcgccc tgcttggctt taccggctgg cgaatgagca aacgcccgca
    32221 ggcagcagag gcggaagaga aaagcctcga aacactgacc cgcaccatca ccgaaaccag
    32281 cgagcaacag gtcagttggg aaaccattcc gcttatcgag cctatcagtt taagcctcgg
    32341 ctacaagctg gtggcgctgg tggacaaagc gcagggcaac ccgctcaccc agcgtattcg
    32401 cggcgtacgg caggtgatat ccgacggtaa cggcgtgctg ctgccggaga tccgcattcg
    32461 ggaaaacttc cgccttaagc cgagccagta cgccattttc atcaacggta ttaaggctga
    32521 tgaagcagat attccggcgg ataaactgat ggccctgccc tccagcgaaa cctacggcga
    32581 gatagacggc gtgctgggga acgacccggc gtacgggatg ccggtgacct ggatccagcc
    32641 cgcgcagaaa gcgaaagcgc tgaatatggg gtatcaggtg atcgacagcg ccagcgtgat
    32701 tgccacccat gtgaacaaga ttgtccgcag ctatattcct gatttgttta actatgatga
    32761 catcacgcag ttgcataacc gtttgtcgtc gatggcaccg cgcctggcgg aggatttaag
    32821 cgcggcgctc aattacagcc aattgctgaa agtgtaccgg gcgctgctga ccgaaggcgt
    32881 ttccctgcgc gatatcgtca ccatcgccac cgtgctggtc gcaagtagca cagtaaccaa
    32941 agatcatatt ctgctggcag ctgatgtgcg cctggcgctg cggcgcagca ttacccatcc
    33001 gttcgttcgc aagcaggagc tgacggtgta tacgctgaat aatgagctgg aaaatctgct
    33061 gaccaacgtg gtgaatcagg cgcaacaggg cggcaaagtg atgctcgaca gcgtgccggt
    33121 cgacccgaac atgctcaacc agttccagag cacgatgccg caggtgaaag agcagatgaa
    33181 agcggcgggg aaagacccgg tgctgctggt gccgccgcag ctgcgccctt tgctggcgcg
    33241 ctacgcaagg ttgtttgcgc cggggctgca tgtgctgtcg tataacgaag tgccggatga
    33301 gctggagttg aagattatgg gggcgttgag ttgattggcc tttaaggggc gcatccggca
    33361 ttggtgcaca attgccggat gcggcgtgaa cgccttatcc ggcctaccga tccggcacag
    33421 gtttgtaggc atgataagac gcgccagcgt cgcatcaggc atccgcgcac aattgccgga
    33481 tgcggcacaa gtttgtaggc atgataagac gcgctagcgt cgcatcaggc atctgcgcac
    33541 aattataaaa tgatacgctc cccagcactc aaatccgtta tgtctcccgc ccaatcgatg
    33601 ggatataacc ctcgcgcaac atcgcgatgg aacgttgaga atggccaatc acacacctgc
    33661 tttacccagc catgtttcac tggatttata taaatataat caacatgatg ccgataatct
    33721 tttgtattac ggatggcatg ctcccaaaaa cgcggttgcc agatattttt caatccgcaa
    33781 gcatgggtga actgcttttt aatctctctc cagcgcgagg aaaaatcatc atcgctttca
    33841 ggtaatgtcc agatacagtg catatgctcc ggcaaaacta cccaggcatt aatttcaaaa
    33901 ggtctggctc gcttaacttt aataatggcg ttacggagcg tctggtactg ggtagtcaaa
    33961 agttggcttc gacgattccg taaattcacc gtgaaaaacc agcttccccc cttgaggtaa
    34021 taacgacgat attcagacat agcgactcct ttttctcaac ggtaagaaga aaaggaaagg
    34081 ttggcaatga ggttgtttta tatcgggaaa gcggctcgca aatattcgcg ggctcgtagg
    34141 catgataaga cgcgccagcg tcgcatcagg cacctgcgta caactgccgg atgcggcgtg
    34201 aatgccttat ccgtcctaca aaatcgtctc ctccgtcaaa atccggcgcg cgcctaaaaa
    34261 atgatcctgc cagaaaggtt cagctaaccg acttacccga atggtttccc cggtgcgcgg
    34321 tgactcaata aactgaccgt cgcccagata cacccccata tggtcggcaa tctcgcggct
    34381 gtggatatgg aaaaacagca aatctccccg gcgcaggtcg ttattcgcca caatcgttgc
    34441 ccgatggtag tgatacatct cattagccgt acgcgggagc ttcgcctcga ggatcttgtt
    34501 gtaggcataa aaaaccaacc cgctacagtc gaagccttta tcgggccgcg tgccgcccca
    34561 gacgtaaggc ttgccaagct gttgctccag gcggtgaatc gccacttcgg tgatgttatg
    34621 caggtgatcg ctgcttaaaa agtggttgtt ttccgccagc gcctgccagc gtctgtcact
    34681 cgccttgagc ggcgcaggaa accattctgg atgctgtttt atctgctccc ggttgtgctg
    34741 gcgtagcgcc cttctgcttt cggcattgcc ttccacaata tagctggcct gttttttcag
    34801 atgggtttgg tactgtttta ataaacgcgc acggttttgc atccgctggc gggcggcgta
    34861 gctgaaagag atatgtgatg tggtggcgct ggcaaatgaa gaaaacggca aacaaatcaa
    34921 cagtaatccc gacaatacaa aggatggaat cgacggcaag gacatactca ataatggctg
    34981 gtcatgcttt aatgttgcag tgtatcccgg atcgtttctg aacaaactta ataaaccatt
    35041 attataaaat taacatatat atttcagtga attatgtaaa tacatcagtt tattgaattg
    35101 ctcataaatt gccgctgttg ctcaattgag ctacaattca agctgaatat atatacagca
    35161 caggagatac cccaatggct gcaaatgcat ttgttcgcgc ccgaatcgat gaagatctga
    35221 agaatcaggc ggcggacgta ctggccggga tggggctgac catctctgac ctggttcgca
    35281 taaccctcac aaaggtcgcg cgtgaaaagg cattgccgtt tgatttacgc gagcctaata
    35341 aattaaccat tcaatcaatc aaaaacagcg aagctggcgt tggtgttcat aaggccaaag
    35401 acgccgatga tttatttgat aaattaggaa tttaaatatg attcaaaggg atattgaata
    35461 ctcgggacaa ttttcaaagg atgtaaaact tgcacaaaag cgtcataagg atatgaataa
    35521 attgaaatat cttatgacgc ttcttatcaa taatatttta ccgcttccag ctgtttataa
    35581 agaccacccg ctgcaaggtt catggaaagg ttatcgcgat gctcatgtcg aaccggactg
    35641 gctcctgatt tacaaactta ccgataaact tttacgattt gagagaactg gaactcacgc
    35701 ggcgctcttt gggtaaagcc cgctgccgat gaaatttgta aaccctgcgc cgacaggctt
    35761 gcgtaaaact cttttggtga taatgtgagc cttccaaatc cagactattc cgatttggga
    35821 tccattttgc cgtcctggcg tgtgtaaccg ttttatcaag gaataagcag tatgcgtaaa
    35881 atcgcattaa ttcttgcgat gttgttgatc ccgtgcgttt cgtttgccgg tttgctgggc
    35941 agcagtagct cgaccacgcc agtgagcaaa gagtataagc agcagttgat gggatcccct
    36001 gtctacatcc agatcttcaa ggaagaacgt acgctcgatc tctacgtcaa aatgggcgag
    36061 caatatcaac tgctcgacag ctataaaatc tgtaaatatt ccggcggctt agggccaaaa
    36121 cagcgtcagg gcgatttcaa aagcccggaa gggttttata gcgtccagcg taatcaatta
    36181 aaaccagaca gccgttacta caaagcgatt aatattggat tccccaatgc ctatgaccgt
    36241 gcgcatggtt atgaagggaa atacctgatg atccacggcg attgtgtttc catcggctgc
    36301 tacgcgatga ccaatcaggg cattgacgag atcttccagt tcgttactgg cgcgctggta
    36361 tttggtcagc cgagcgtgca ggtgagcatt tacccgttcc gcatgaccga cgctaatatg
    36421 aagcgccaca aatattccaa ctttaaggac ttctgggagc aactgaagcc aggctacgac
    36481 tactttgagc aaacccgtaa gccaccgacc gtttctgtgg tcaatggccg ttacgtggtc
    36541 agcaagccgt taagccacga agttgtgcaa ccacaactgg catcaaacta cacgctcccc
    36601 gaggcaaaat aagcgccatt cgcctggcat aatcttttgc caggtttcat tgcccgtcag
    36661 aggctgtgtc gcaatcaccg tgaccacatc attcggtgtg gtctgcgagc tgaagtcgat
    36721 ttctacatcc tgatccagca acgttgccac gccaaacggc gcgcggcggg tgatccagtg
    36781 taaattagtc gagcaatacg ccattacata gcgcccgtca gaaagcagca tgttgaaaac
    36841 gcccttctgc cgcagttcat ccgccagtga ggcgatatat ttaaataccg ccgccatgtt
    36901 gcccggtgtg cgcgggtaac gctgcgttaa tttatgcagg agccagcaga aggctttttc
    36961 gctgtcggtt tcgcctaccg ggcggaagtt gccggtttcc agtgatttgt agcccgtcag
    37021 ttgtccgtta tgggcataag tccagttacg cccccataac tcgcgggtaa atgggtgagt
    37081 attttccagc gccacctcgc cccgattagc ctggcgaata tgagccacca ccgaacagga
    37141 ttttatcggg tagtcctgga ccagtttggc gataggtgag ttaaagctgg gttgcggatc
    37201 tttaaatgtg cgacagcctt taccttcgta aaaggtaatg ccccagccat ctttatgtgg
    37261 cccggttcca ccaccacgct gcaccagacc ggtgaaacta aagcagatat cggtagggac
    37321 gttggcgctc attccgagca attcgcacat gggtaacctc cacaacagcg ggagcgcatg
    37381 gcccccggcg aagattgatt taagaatttt caggtcggat gcggcgtgaa cgcattattc
    37441 ggcctacggt tcgcgtacag atttgtaggc atgataagac gcgtcagcgt cgcatcaggc
    37501 atcagcgcac aaatgccgga tgcggcgtaa acgtcttatc cggcctacgc cagacttact
    37561 taaccatctc tttttcaatt aactggatca ggatatggat aactttaatg tgaatttcct
    37621 gaatgcggtc ggcataacca aagtgcggaa cgcgaatttc gatatccgcc gtgccagcca
    37681 ttttgccgcc gtctttacca gtcagggtga tcactttcat ccccttctca cgcgccgctg
    37741 cgatcgcttt gatcacgttt gcagaattac cggaggtgga gatccccagc agtacatcgc
    37801 cttcgcgacc taccgcttca acgtaacggg agaaaatatc attgaaaccg aaatcgttac
    37861 cgacgcagga aatatgacta acgtcagaaa tagcaatcgc cgggtagccc ggacggtttt
    37921 cacggtagcg accggtcaac tcttcggcaa agtgcatggc gtcgcaatgg gagccgccgt
    37981 tgccgcagga aagcactttg ccaccggctt taaagctatc tgctaacagg accgccgcgc
    38041 gctgaatggc gtgaatattg gcgtcatctt ttaaaaagtt agccagcgtt tccgccgctt
    38101 cgttcagttc gttacgaata agatcctggt acatgaggat atccttcagc ataaatgtaa
    38161 tagacaaaat gcagtgtacc ggataccgcc aaaagcgaga agtacgggca ggtgctatga
    38221 ccaggacttt ttgacctgaa gtgcggataa aaacagcaac aatgtgagct ttgttgtaat
    38281 tatattgtaa acacattgct aaatgttttt acatccacta caaccatatc atcacaagtg
    38341 gtcagacctc ctacaagtaa ggggcttttc gttatgatga ttttgagtat tctcgctacg
    38401 gttgtcctgc tcggcgcgtt gttctatcac cgcgtgagct tatttatcag cagtctgatt
    38461 ttgctcgcct ggacagccgc cctcggcgtt gctggtctgt ggtcggcgtg ggtactggtg
    38521 cctctggcca ttatcctcgt gccatttaac tttgcgccta tgcgtaagtc gatgatttcc
    38581 gcgccggtat ttcgcggttt ccgtaaggtg atgccgccga tgtcgcgcac tgagaaagaa
    38641 gcgattgatg cgggcaccac ctggtgggag ggcgacttgt tccaaggcaa gccggactgg
    38701 aaaaagctgc ataactatcc gcagccgcgc ctgaccgctg aagagcaagc gtttctcgac
    38761 ggcccggtag aagaagcctg ccggatggcg aatgatttcc agatcaccca tgaactggcg
    38821 gatctgccgc cagagttgtg ggcgtacctt aaagagcatc gcttcttcgc gatgatcatc
    38881 aaaaaagagt acggcgggct ggagttctcg gcttatgccc agtctcgtgt gctgcaaaaa
    38941 ctctccggcg tgagcgggat cctggcgatt accgtcggcg tgccgaactc attaggcccg
    39001 ggcgagctgc tgcaacatta cggcactgac gagcagaaag atcactatct gccgcgtctg
    39061 gcgcgtggtc aggaaatccc ttgttttgcg ctaaccagcc cggaagcggg ttccgatgcg
    39121 ggcgctatcc cggataccgg gattgtctgc atgggtgaat ggcagggcca gcaggtgctg
    39181 gggatgcgtc tgacctggaa caaacgctac attacgctgg caccgattgc gaccgtgctt
    39241 ggtctggcgt ttaaactctc cgacccggaa aaattgctgg gtggtgcaga agatttaggt
    39301 attacctgtg cgctgatccc gaccaccacg ccgggcgtgg aaattggtcg tcgccacttc
    39361 ccgctgaacg tacctttcca gaacggaccg acgcgcggta aagatgtctt cgtaccgatc
    39421 gattacatca tcggcggacc caaaatggcc gggcaaggct ggcggatgct ggtggagtgc
    39481 ctctcggtag gccgcggcat caccttgcct tccaactcaa ccggcggcgt gaaatcagta
    39541 gcgctggcaa cgggtgcgta tgcgcacatt cgtcgtcagt tcaaaatttc tattggtaag
    39601 atggaaggga ttgaagagcc gctggcgcgt attgccggga acgcttatgt aatggatgct
    39661 gcggcatcgc tgattaccta cggcattatg ctcggcgaaa aacctgccgt gctgtcggct
    39721 atcgttaagt atcactgtac ccaccgcggg cagcagtcga ttattgatgc gatggatatc
    39781 actggtggca aaggcattat gctcgggcaa agcaacttcc tggcgcgtgc ttaccagggc
    39841 gcaccgattg ccatcaccgt tgaaggggct aacattctga cccgcagcat gatgatcttc
    39901 ggacaaggag cgattcgttg ccatccgtac gtgctggaag agatggaagc agcgaagaac
    39961 aatgacgtta acgcgttcga taaactgttg ttcaaacata tcggtcacgt cggtagcaac
    40021 aaagttcgca gcttctggct gggcctgacg cgcggtttaa ccagcagcac gccaaccggc
    40081 gatgccacta aacgctacta tcagcatctt aaccgcctga gcgccaacct cgccctgctt
    40141 tctgatgtct cgatggcagt gctgggcggc agcctgaaac gtcgtgagcg tatctcggca
    40201 cgtctggggg atattttaag ccagctttac ctcgcctctg ccgttctgaa gcgttatgac
    40261 gacgaaggcc gtaatgaagc cgatctgccg ctggtgcact ggggcgtaca ggatgcgctg
    40321 tatcaggcag aacaggcgat ggacgactta ctgcaaaact tcccgaaccg cgtggttgcc
    40381 gggctgctga atgtggtgat cttcccgacc ggacgtcatt atctggcacc gtctgacaag
    40441 ctggatcata aagtggcgaa gattttacaa gtgccgaacg ccactcgttc acgtattggt
    40501 cgcggtcagt acctgacgcc aagcgagcat aacccggttg gtttactgga agaggcgttg
    40561 gtggatgtga ttgccgccga cccgattcat cagcggatct gtaaagatct gggtaaaaac
    40621 ctgccattta cccgtctgga tgaactggcg cacaacgcgc tggcgaaggg gctgattgat
    40681 aaagatgaag ccgctattct ggtgaaagct gaagaaagcc gtctgcgcag tattaacgtt
    40741 gatgactttg atccggaaga gctggcgacg aagccggtaa agttgccgga gaaagtgcgg
    40801 aaagttgaag ccgcgtaatt gaataacgga gccgaaaggc tccgtttctt tatccgctaa
    40861 ttatttaaaa ttaaagccat ccggatggtt ttccaggctg ccggtcaacg ccgcgaacag
    40921 caccgtttta ccatcaatcg aaagcgcatc gttcacattc aaccaggtga gtttctcttg
    40981 cgacgttttc tcatcaatag tcgagaacag ccccgtcatc tgattagatt tctcggacca
    41041 catcacagcg atacgttgcg agccacagtc atgcggtttg cacgcgctca tcacctgata
    41101 cgtctcatct cccaacgtta cggtttgtgc gggagtataa gtaccgcctt tcatcaccca
    41161 tgcaggtagc ttatgccctt gcaccatctg attaaacgca gctttggtgg tttcgccctt
    41221 tgcaaggctg ctaatggtta aatcatcctg cgccattgca ctggtggcga tgaccagagc
    41281 ggcgactgtc gttattgcct taaacatcat tcctcccgag cttatcctgc ccatgttatt
    41341 aacctcctaa agcctggcga taacacgata atttgcaaat cagatatttc caatatcagc
    41401 atttgttatc aatttattct gcgctaaggc aactgcctga taaggtgtta aagttataaa
    41461 acccttcgca taaaggagtt gtacaccgtg cctggtttga agattacgct tttgcagcaa
    41521 ccgttggtgt ggatggatgg tcctgccaac ctgcgtcatt ttgatcgtca actggaaggt
    41581 attaccgggc gcgatgtgat cgttctaccg gagatgttta ccagcggctt tgccatggaa
    41641 gcggcagctt cgtcgctagc acaaaatgac gtagtgaact ggatgacagc taaagcgcag
    41701 cagtgcaatg cgctgattgc aggcagtgtt gcattacaaa cggagtctgg ttcggttaac
    41761 cgctttttgc tggttgagcc gggcggcacg gtacattttt atgataagcg tcatctgttc
    41821 cgcatggcag atgagcatct acattataaa gcgggcaatg cgcgagtgat tgtggaatgg
    41881 cgcggctggc gtattttgcc gctggtgtgc tacgacttac gttttcctgt gtggtcgcgc
    41941 aatctcaacg attatgacct cgccctgtac gtcgccaact ggcctgctcc gcgctctctg
    42001 cactggcagg cattgctgac ggcccgcgcg attgagaatc aggcgtatgt ggcgggatgc
    42061 aatcgcgtcg gcagcgatgg caacggctgc cattatcgcg gtgacagccg ggtgattaat
    42121 ccacaaggcg agattatcgc tactgccgac gcgcatcagg caacgcgcat tgatgcggag
    42181 ctgtcgatga tggcattgcg ggaatataga gaaaagtttc cggcatggcg ggatgcggat
    42241 gagtttaggt tgcggtgagt gggtgttgtg tttagggccg gggagagtcg gggtatgact
    42301 acgaaagccc gctctcctgc gaccactttt atcataggag tgtggaagcg cttttccatc
    42361 aatcgcgatg acgtctttgt catttgatga atggcagtca cgcatccagt taataaagca
    42421 ctcgtgaa
//
LOCUS       DAPGRN010000029        42274 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPGRN010000029 DAPGRN010000000
VERSION     DAPGRN010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.029"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            506..592
                     /gene="leuL"
                     /locus_tag="RHW33_001708"
     CDS             506..592
                     /gene="leuL"
                     /locus_tag="RHW33_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV1645220.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            685..2256
                     /gene="leuA"
                     /locus_tag="RHW33_001709"
     CDS             685..2256
                     /gene="leuA"
                     /locus_tag="RHW33_001709"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV1645221.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2256..3347
                     /gene="leuB"
                     /locus_tag="RHW33_001710"
     CDS             2256..3347
                     /gene="leuB"
                     /locus_tag="RHW33_001710"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV1645222.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     IELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3350..4750
                     /gene="leuC"
                     /locus_tag="RHW33_001711"
     CDS             3350..4750
                     /gene="leuC"
                     /locus_tag="RHW33_001711"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV1645223.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4761..5366
                     /gene="leuD"
                     /locus_tag="RHW33_001712"
     CDS             4761..5366
                     /gene="leuD"
                     /locus_tag="RHW33_001712"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV1645224.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMR"
     gene            complement(5474..5605)
                     /gene="sgrT"
                     /locus_tag="RHW33_001713"
     CDS             complement(5474..5605)
                     /gene="sgrT"
                     /locus_tag="RHW33_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV1645225.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            5694..7352
                     /gene="sgrR"
                     /locus_tag="RHW33_001714"
     CDS             5694..7352
                     /gene="sgrR"
                     /locus_tag="RHW33_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV1645226.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHARP
                     THCEKPAGLEHLTLTFYQDHIEHRVIARIMQQILASHQVTMEIKEISYDQWHKGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPLDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDPE"
     gene            complement(7581..8150)
                     /gene="espY2"
                     /locus_tag="RHW33_001715"
     CDS             complement(7581..8150)
                     /gene="espY2"
                     /locus_tag="RHW33_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspY2"
                     /protein_id="HDV1645227.1"
                     /translation="MKVRNPEQISIPASNTTKDPGLTNSQIIRMANLVKKTENMNIFE
                     ELWETLRNLFRSDKHSQTAARQILKDAFYFQNSDDYSKYFTGAVDGKVRDQLTHWLIK
                     FNELKEYAKDPENMAAKASLSPEGTLCVSFFIGDEAIFTLELQLKKSTRTGGIDLSNA
                     YFNGVVICGIDCLEVDLSNAETNNSRWYD"
     regulatory      8392..8491
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8522..9505
                     /gene="thiB"
                     /locus_tag="RHW33_001716"
     CDS             8522..9505
                     /gene="thiB"
                     /locus_tag="RHW33_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV1645228.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDIFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDHYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEKLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9481..11091
                     /gene="thiP"
                     /locus_tag="RHW33_001717"
     CDS             9481..11091
                     /gene="thiP"
                     /locus_tag="RHW33_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV1645229.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWS
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVTALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNSTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            11075..11773
                     /gene="thiQ"
                     /locus_tag="RHW33_001718"
     CDS             11075..11773
                     /gene="thiQ"
                     /locus_tag="RHW33_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV1645230.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(11887..12651)
                     /locus_tag="RHW33_001719"
     CDS             complement(11887..12651)
                     /locus_tag="RHW33_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1645231.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLVRHPLMPVYIDILRKVVGV"
     gene            complement(12737..13615)
                     /gene="araC"
                     /locus_tag="RHW33_001720"
     CDS             complement(12737..13615)
                     /gene="araC"
                     /locus_tag="RHW33_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV1645232.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13953..15653
                     /gene="araB"
                     /locus_tag="RHW33_001721"
     CDS             13953..15653
                     /gene="araB"
                     /locus_tag="RHW33_001721"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV1645233.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCTTGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15664..17166
                     /gene="araA"
                     /locus_tag="RHW33_001722"
     CDS             15664..17166
                     /gene="araA"
                     /locus_tag="RHW33_001722"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV1645234.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAAEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17366..18061
                     /gene="araD"
                     /locus_tag="RHW33_001723"
     CDS             17366..18061
                     /gene="araD"
                     /locus_tag="RHW33_001723"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV1645235.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLNKH
                     YLRKHGAKAYYGQ"
     gene            18136..20487
                     /gene="polB"
                     /locus_tag="RHW33_001724"
     CDS             18136..20487
                     /gene="polB"
                     /locus_tag="RHW33_001724"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV1645236.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQARGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNVWWAETLQKQQLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20651..23557
                     /gene="rapA"
                     /locus_tag="RHW33_001725"
     CDS             20651..23557
                     /gene="rapA"
                     /locus_tag="RHW33_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV1645237.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23569..24228
                     /gene="rluA"
                     /locus_tag="RHW33_001726"
     CDS             23569..24228
                     /gene="rluA"
                     /locus_tag="RHW33_001726"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV1645238.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            24441..24613
                     /locus_tag="RHW33_001727"
                     /pseudo
     CDS             24441..24613
                     /locus_tag="RHW33_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24646..25398)
                     /locus_tag="RHW33_001728"
     CDS             complement(24646..25398)
                     /locus_tag="RHW33_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP pyrophosphate phosphatase"
                     /protein_id="HDV1645239.1"
                     /translation="MKVSVPGMPATLLNMSNNDIYKMVSGDKIDVKMNIFQRLWETLR
                     HLFWSDKQTEAYKLLFNFVNKKAGNINASKYFTGAVNENEKEKFINSLELFNELKTCA
                     KNPDEMVAKGSMSWVAQTFGDIELSVTFFIENKEICTQTLQLHKGPGNLGVDLGEAYL
                     PGVDMRDCYLGLKTMKGHNKLLYLEPGWNANLDGATLDGSILDGATVDGATHLYDEVI
                     IINKITPKKIATEEIATKQSTAEEIIANAIIE"
     gene            complement(25693..26508)
                     /gene="djlA"
                     /locus_tag="RHW33_001729"
     CDS             complement(25693..26508)
                     /gene="djlA"
                     /locus_tag="RHW33_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV1645240.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            26763..29132
                     /gene="lptD"
                     /locus_tag="RHW33_001730"
     CDS             26763..29132
                     /gene="lptD"
                     /locus_tag="RHW33_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV1645241.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSNPQNNKLADSVNRVMPQFKVDGKMVFERDMNLLAPGFTQT
                     LEPRVQYLYVPYRDQSDIYNYDSALLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSR
                     IYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLR
                     GGIQYDTRLDNVATSSSSIEYRRDEDRLVQLNYRYASPEYIQATLPRDSRDKRWNAPQ
                     YKDGISQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERK
                     LNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            29185..30471
                     /gene="surA"
                     /locus_tag="RHW33_001731"
     CDS             29185..30471
                     /gene="surA"
                     /locus_tag="RHW33_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV1645242.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            30471..31460
                     /gene="pdxA"
                     /locus_tag="RHW33_001732"
     CDS             30471..31460
                     /gene="pdxA"
                     /locus_tag="RHW33_001732"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV1645243.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            31457..32278
                     /gene="rsmA"
                     /locus_tag="RHW33_001733"
     CDS             31457..32278
                     /gene="rsmA"
                     /locus_tag="RHW33_001733"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV1645244.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            32281..32658
                     /gene="apaG"
                     /locus_tag="RHW33_001734"
     CDS             32281..32658
                     /gene="apaG"
                     /locus_tag="RHW33_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV1645245.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            32665..33513
                     /gene="apaH"
                     /locus_tag="RHW33_001735"
     CDS             32665..33513
                     /gene="apaH"
                     /locus_tag="RHW33_001735"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV1645246.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(33510..33824)
                     /locus_tag="RHW33_001736"
     CDS             complement(33510..33824)
                     /locus_tag="RHW33_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HDV1645247.1"
                     /translation="MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFI
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(33827..34060)
                     /locus_tag="RHW33_001737"
     CDS             complement(33827..34060)
                     /locus_tag="RHW33_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HDV1645248.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(34146..34625)
                     /gene="folA"
                     /locus_tag="RHW33_001738"
     CDS             complement(34146..34625)
                     /gene="folA"
                     /locus_tag="RHW33_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV1645249.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34817..36679)
                     /gene="kefC"
                     /locus_tag="RHW33_001739"
     CDS             complement(34817..36679)
                     /gene="kefC"
                     /locus_tag="RHW33_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV1645250.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36672..37202)
                     /gene="kefF"
                     /locus_tag="RHW33_001740"
     CDS             complement(36672..37202)
                     /gene="kefF"
                     /locus_tag="RHW33_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV1645251.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAVYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(37313..38644)
                     /locus_tag="RHW33_001741"
     CDS             complement(37313..38644)
                     /locus_tag="RHW33_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645252.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38702..38989)
                     /gene="fixX"
                     /locus_tag="RHW33_001742"
     CDS             complement(38702..38989)
                     /gene="fixX"
                     /locus_tag="RHW33_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV1645253.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38986..40272)
                     /locus_tag="RHW33_001743"
     CDS             complement(38986..40272)
                     /locus_tag="RHW33_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1645254.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAIRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(40323..41264)
                     /locus_tag="RHW33_001744"
     CDS             complement(40323..41264)
                     /locus_tag="RHW33_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV1645255.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(41279..42049)
                     /locus_tag="RHW33_001745"
     CDS             complement(41279..42049)
                     /locus_tag="RHW33_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV1645256.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDV
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10151 a        11504 c        10990 g         9629 t
ORIGIN      
        1 aatccattgc cgtggaaagc gtcatttacc acagcataat aatccataat gttcttgttg
       61 cgtaaacaat ctaatcataa ctaccgcgaa tactcaatca tccacaaaat ggattaaatg
      121 tgctttttaa taatttattt atatgtttaa tgcattaatt cttaacatta attgatcaat
      181 aatattcacc aaatcaatat caaaaaatcc gcaaaacata taattcaata caaatcatca
      241 ggataggttt tgcaacgcgt gcattttgtc ccctttttcc tcgtggatta gatgcaaaaa
      301 tttattctga aatatgtcaa ccgatgaaaa gcatcggtag ttaagcagaa attaatatcg
      361 cttgttttaa ccacagcagc acaattagct aatcttacgg atgcagaact catgctggcg
      421 ggacgttttt attgcgtcag ggttgacatc cgtttttgta tccagtaact ctaaaagcat
      481 atcgcattca tctggagctg atttaatgac tcacatcgtt cgctttatcg gtctactact
      541 actaaacgca tcttctttgc gcggtagacg agtgagcggc atccagcatt aagccagcac
      601 gcagtcaaac aaaaaacccg cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct
      661 ctaaaagaga caaggaccca aaccatgagc cagcaagtca ttattttcga taccacattg
      721 cgcgacggtg aacaggcgtt acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg
      781 ctggcccttg agcgtatggg tgttgacgtg atggaagtcg ggttccccgt ctcttcgccg
      841 ggtgattttg aatcagtgca aaccatcgct cgccaggtta aaaacagccg tgtatgcgcg
      901 ttagctcgct gcgtggagaa agatatcgac gtggcggccg aatccctgaa agtcgccgaa
      961 gccttccgta ttcatacctt tattgccact tcgccaatgc acatcgccac caagctgcgc
     1021 agcacgctgg acgaggtgat cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac
     1081 accgatgatg ttgaattttc ttgcgaagat gccgggcgta cacccattgc cgatctggcg
     1141 cgagtggtcg aagcggcgat taatgccggt gccaccacca tcaacattcc ggacaccgtg
     1201 ggctacacca tgccgtttga gttcgccgga atcatcagcg gcctgtatga acgcgtgcct
     1261 aacatcgaca aagccattat ttccgtacat acccacgacg atttgggcct ggcggtcggc
     1321 aactcactgg cggcggtaca tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc
     1381 ggcgagcgtg ccggtaactg ttccctggaa gaagtcatca tggcgattaa agttcgcaag
     1441 gatattctca acgtccacac cgccattaat caccaggaga tatggcgcac cagccagtta
     1501 gttagccaga tttgtaatat gccgatcccg gcaaacaaag ccattgttgg cagcggcgca
     1561 ttcgcacact cctccggtat ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa
     1621 atcatgacac cagaatctat tggtctgaac caaatccagc tgaatctgac ctctcgttcg
     1681 gggcgtgcgg cggtgaaaca tcgcatggat gagatggggt ataaagaaag tgaatataat
     1741 ttagacaatt tgtacgacgc cttcctgaag ctggcggaca aaaaaggtca ggtgtttgat
     1801 tacgatctgg aggcgctggc cttcatcggt aagcagcaag aagagccgga gcatttccgt
     1861 ctggattact tcagcgtgca gtctggctct aacgatatcg ccaccgccgc cgtcaaactg
     1921 gcctgtggcg aagaagtcaa agcagaagcc gccaacggta acggtccggt cgatgccgtc
     1981 tatcaggcaa ttaaccgcat cactgactat aacgtcgaac tggtgaaata cagcctgacc
     2041 gctaaaggtc acggtaaaga tgcgctgggt caggtggata ttgtcgccaa ctacaacggt
     2101 cgccgcttcc acggcgtcgg cctggccacc gatattgtcg agtcctccgc caaagccatg
     2161 gtgcacgtac tgaacaatat ctggcgcgcc gcagaagtcg aaaaagagtt gcaacgcaaa
     2221 gctcaacaca acgaaaacaa caaggaaacc gtgtgatgtc gaagaattac catattgccg
     2281 tattgccggg ggacggtatt ggtccggaag tgatgaccca ggcgctgaaa gtgctggatg
     2341 ccgtgcgcaa ccgctttgcg atgcgcatca ccaccagcca ttacgatgta ggcggcgcag
     2401 ccattgataa ccacgggcag ccgctgccgc ctgcgacggt tgaaggttgt gagcaagccg
     2461 atgccgtgct gtttggctcg gtaggcggcc cgaagtggga acatttacca ccggaccagc
     2521 aaccagaacg cggcgcactg ttgcctttgc gtaagcactt caaattattc agcaacctgc
     2581 gtccggcaaa actgtatcag gggctggaag cattctgtcc gctgcgtgct gacattgccg
     2641 ctaacggctt cgacatcctg tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc
     2701 caaaaggccg cgaaggtagc ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc
     2761 gttttgagat tgaacgtatc gcccgcatcg cgtttgaatc tgctcgcaag cgtcgccaca
     2821 aagtcacctc aatcgacaaa gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg
     2881 tcaacgagat cgccacggaa tacccggata tcgaactggc gcatatgtac atcgacaacg
     2941 ccaccatgca gctgattaaa gatccatcac agtttgacgt cctgctgtgc tccaacctgt
     3001 ttggcgacat tctgtctgac gagtgcgcaa tgatcactgg ctcgatgggc atgttgcctt
     3061 ccgccagcct gaacgagcaa ggttttggtc tgtatgaacc ggcaggcggc tcggcaccag
     3121 atatcgcagg caaaaacatc gccaacccga ttgcgcaaat tctgtcgctg gcactgctgc
     3181 tgcgctacag cctggatgcc gatgatgcgg cttccgccat tgaacgcgcc attaaccgcg
     3241 cattagaaga aggcattcgc accggggatt tagcccgtgg cgctgccgcc gttagtaccg
     3301 atgaaatggg cgatatcatt gcccgctatg tggcagaagg ggtgtaatca tggctaagac
     3361 gttatacgaa aaattgtttg acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct
     3421 gttatatatc gaccgccacc tggtgcatga agtgacctca ccgcaggcgt tcgatggtct
     3481 gcgcgcccac ggtcgcccgg tacgtcagcc ggggaaaacc ttcgccacca tggatcacaa
     3541 cgtttcaact cagaccaaag acattaatgc ctgcggtgaa atggcgcgca tccagatgca
     3601 ggagctgatc aaaaactgca aagaatttgg cgtcgagctg tatgacctga atcaccccta
     3661 tcaggggatc gtccacgtaa tggggccgga acagggcgtg accttgccgg ggatgaccat
     3721 tgtctgcggc gactcgcata ccgccaccca cggcgcgttt ggcgcactgg cctttggtat
     3781 cggcacttcc gaagttgaac acgtactggc aacgcaaacc ctgaaacagg gccgtgcgaa
     3841 gaccatgaaa attgaagtcc agggcaaagc cgcgccgggc attaccgcaa aagatatcgt
     3901 gttggcaatt atcggtaaaa ctggcagcgc aggcggcacc gggcatgtgg tggagttttg
     3961 cggcgaagca atccgtgatt taagcatgga aggtcggatg accctgtgca atatggcaat
     4021 cgaaatgggc gcgaaagccg gtctggttgc accggacgaa accaccttta actatgtcaa
     4081 aggtcgtctg catgcgccga aaggcaaaga tttcgacgac gccgttgcct actggaaaac
     4141 cctgcaaacc gacgaaggcg caactttcga taccgttgtc actctgcaag cagaagagat
     4201 ttcgccgcag gtcacctggg gcaccaatcc aggccaggtg atttccgtga acgacaatat
     4261 tcccgatccg gcttcgtttg ccgatccggt tgaacgtgcg tcggcagaaa aagcgctggc
     4321 ctatatgggg ctgaaaccgg gtattccgct gaccgaagtg gctatcgaca aagtgtttat
     4381 cggttcctgt accaactcgc gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg
     4441 gcgaaaagtc gcgccaggcg tgcaggcact ggtggttccc ggctctggcc cggtaaaagc
     4501 ccaggcggaa gcggaaggtc tggacaaaat ctttattgaa gccggttttg aatggcgctt
     4561 gcctggctgc tcaatgtgtc tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg
     4621 tgcctccacc agcaaccgta actttgaagg ccgccagggg cgcggcgggc gcacgcatct
     4681 ggtcagcccg gcaatggctg ccgccgcggc tgtgaccgga catttcgccg acattcgcaa
     4741 cattaaataa ggagcacacc atggcagaga aatttatcaa acacacaggc ctggtggttc
     4801 cgctggatgc cgccaatgtc gataccgatg caatcatccc gaaacagttt ttgcagaaag
     4861 tgacccgtac gggttttggc gcgcatctgt ttaacgactg gcgttttctg gatgaaaaag
     4921 gccaacagcc aaacccggac ttcgtgctga acttcccgca gtatcagggt gcttccattt
     4981 tgctggcgcg ggaaaacttc ggctgtggct cctcgcgtga gcacgcgccc tgggcattga
     5041 ccgactacgg ttttaaggtg gtgattgcgc cgagttttgc tgacatcttc tacggcaata
     5101 gctttaacaa ccagctgctg ccggtgaaat taagcgacgc agaagtggat gagctgtttg
     5161 cgctggtgaa agccaatccg gggattcatt ttgatgtaga cctggaagcg caagaggtga
     5221 aagcgggtga gaaaacctat cgctttacca ttgatgcctt ccgccgccac tgcatgatga
     5281 acggtctgga cagcattggg cttaccttgc agcacgacga cgccattgcg gcttatgaag
     5341 cgaagcaacc ggcgtttatg cgttagtttg tacaccgtcc ggtactcaat cgccaaaaag
     5401 aaaaaagcca gcagataatc actgctggcg tggcgattaa caccaatact cagtcacaca
     5461 tgatgtccgc aaatcagtag tcagaatgac tcccctccca ctgcatcagt tcttcaagca
     5521 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     5581 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     5641 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     5701 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     5761 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgcaccc
     5821 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgtggta
     5881 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     5941 acctgctgga gcaggaccgt atcgaccaac tagtgcagtt agttggcgac aaagcgactg
     6001 tgaggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     6061 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     6121 aaacccatat cgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg
     6181 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     6241 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     6301 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgctgac attgtgtcgc
     6361 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttattgc
     6421 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     6481 gccatcccat cggcaccggt ccgtatgcgg tgattcgcaa cagcaccaat caactgaaaa
     6541 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     6601 ttctgccgga aattgccgac gagccagccg gagggctgat gctaaaaggt ccacagggcg
     6661 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgatagcc
     6721 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     6781 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggtttccg gcctatgggc
     6841 tgctcccccg ctggcaccac gcccgcccaa cacattgcga aaaacctgcc gggctggaac
     6901 atctcaccct gacgttttat caggatcata ttgaacatcg ggtgattgcc aggatcatgc
     6961 agcagattct ggcgagtcat caggtcacga tggaaatcaa agagatcagc tacgatcaat
     7021 ggcacaaagg tgagatcgaa agcgacatat ggcttaacag cgccaacttt acgctgccgc
     7081 tggatttttc gctgttcgcg cacttgtgcg aagtacccct gctgcaacac tgtattccgc
     7141 tcgactggca agccgacgcc gcccgctggc gcaatggcga gatgaatctg gcgaactggt
     7201 gccagcaact ggtcgccagt aaagcaatgg tgccacttat ccaccactgg ctgatcattc
     7261 aggggcaacg cagtatgcgc ggcctacgca tgaacaccct cggctggttc gattttaaat
     7321 cagcgtggtt tgcgccgccg gatccagagt agcttgcgcc ttttggcggt catgagcgca
     7381 ttttttatat tcagacggaa tgataaaata cattactttt atattttctc tgttttatgc
     7441 atcgtctgat tagcgtcgca aataagatta aagaaggggc attccagccc ctctttcata
     7501 ttctgagtta atcaataacg gcgcagtcta gccttgttct ggccggaaag gtcagctttt
     7561 tcaggttaac ctcgcgcagg tcagtcatac caacggctat tgttcgtttc agcattgctc
     7621 aggtcaacct cgagacagtc aataccacaa atgacaaccc cgttaaagta agcattactc
     7681 agatcaatcc ccccggtcct ggtgctcttt ttcagctgca actccagagt aaatatcgcc
     7741 tcatcgccaa taaaaaaact gacgcataac gtaccttctg gcgagagtga ggccttcgcg
     7801 gccatatttt ccggatcttt tgcgtattct ttgagttcat taaattttat cagccagtga
     7861 gttaactgat cacgcacctt cccgtcgaca gccccggtaa agtattttga gtagtcatca
     7921 ctattctgga aataaaatgc atccttcaga atttgccttg ccgcagtctg ggaatgttta
     7981 tctgaccgga agagattgcg taacgtctcc catagctctt caaagatatt catattctcg
     8041 gtctttttga ccaagttagc catcctgata atctgagaat tagtaaggcc tggatcttta
     8101 gtggtattac tagcagggat actaatctgt tctgggtttc ttactttcat gctgatagct
     8161 ccactctgag taaaaaactc agaataagcg tcctgaataa gaatactgtc aaaaatcgct
     8221 cattcacgca ataaacggtt acgttaccta ttttgactgt agcgaaggga ttctttcgga
     8281 tcaacgctct ccagctatgc aaatacgctc ttgatagctg aacgaatatt ggctttttta
     8341 atacgccttc tctatggatt gctggttgat aacaaaatca ctacactaac gccgttctca
     8401 acggggtgcc acgcggacgc gtgcgctgag aaaatacccg tcgaacctga tccggataac
     8461 gccggcgaag ggatttgagg ctccttctca agtcctttgc cactcttttt tgaggtgcaa
     8521 agtgttaaaa aaatgtcttc ccctgctgtt gctgtgcaca gcgcccgttt tcgctaaacc
     8581 cgttctgact gtttatacct acgattcctt cgccgccgac tgggggcctg gtccggtggt
     8641 taaaaaagcc tttgaagccg actgtaattg cgaactgaaa ctggtggcgc tggaagatgg
     8701 cgtttcgctt ctcaaccgtc tacggatgga aggcaaaaac agtaaagccg atgtggtgct
     8761 ggggctggat aacaacctgt tagacgccgc cagtaaaacc ggactgtttg ccaaaagcgg
     8821 tgtggcagcg gatgccgtta acgttcccgg cggctggaat aatgacattt tcgtaccgtt
     8881 tgattatggc tacttcgcct tcgtttatga caagaacaaa ctgaaaaacc cgccacaaag
     8941 cctgaaagaa ctggttgaga gcgatcaaaa ctggcgggtg atttatcagg atccgcgcac
     9001 cagtacaccg gggctgggtc tgttgctatg gatgcaaaaa gtctatggcg atgacgcccc
     9061 acaagcctgg cagaaactgg cgaagaaaac ggtcacggtc accaaaggct ggagcgaagc
     9121 ctacggcctg tttttaaaag gtgaaagcga tctggtactg agttacacca cctctccggc
     9181 ttatcacatt ctcgaagaga agaaagatca ctacgccgcc gcgaacttca gcgaaggtca
     9241 ctatctgcaa gtggaagtcg ccgctcgcac cgctgccagc aagcagccgg agctggcgca
     9301 aaaattcctc cagtttatgg tttctccggc tttccagaat gcgatcccaa ccggcaactg
     9361 gatgtatccg gtggcaaacg tcacgctgcc tgccggtttt gaaaaattga ccaaacccgc
     9421 aaccacgctg gagttcacgc cagccgaagt ggcggcacaa cgtcaggcat ggattagcga
     9481 atggcaacgc gccgtcagcc gttaattcca ggctggttaa ttccaggtgt aagcgccgcc
     9541 acgctggtgg tagcggttgc gctggcggcg tttctcgccc tgtggtggaa cgcgccgcag
     9601 ggtgactggt cggcagtctg gcaggacagc tacctgtggc atgtggtgcg cttctccttc
     9661 tggcaggcgt ttctctcggc gctactctct gtcgtacccg cgatattcct cgcccgtgcg
     9721 ctctatcgca ggcgctttcc gggtcggctg gcgctgttgc gtctgtgcgc aatgaccttg
     9781 atcctcccgg tgctggttgc tgttttcggc attcttagcg tctatggtcg ccagggctgg
     9841 ctggcatcgc tctgccaatc gctcggtctg gagtggacct tttcgcccta cggcctgcaa
     9901 ggtattttgc tggcgcacgt attttttaat ctaccgatgg cgagccgctt attactccag
     9961 gcactggaaa acattcccgg cgaacaacgt cagcttgccg cccagcttgg gatgcgcggc
    10021 tggcattttt tccgcttcgt cgaatggccg tggttacggc gacaaatccc gccggttacc
    10081 gcgcttatct ttatgctctg tttcgccagc ttcgccaccg tactgtcgct gggcggcggt
    10141 ccgcaggcaa ccactatcga gctggcaatc tatcaggcgc tgagttacga ctacgatcct
    10201 gcccgcgcgg cgatgctggc gctgatccag atggtgtgtt gccttgggct ggtactgttg
    10261 agtcagcgat tgagtaaggc cattgcgcca ggcaccacgc tgctgcaagg ctggcgcgac
    10321 ccggacgatc gtctgcatag ccgcatttgc gacacggtgt taattgtgct ggcgctgctg
    10381 ctgttgcttc caccgttact ggcggtgatc gtcgatgggg taaatcgcca gttgccggaa
    10441 gtgctggcac aaccggtgct gtggcaggcg ctgtggactt cgttgcgtat tgcgctggcg
    10501 gcaggtgtat tgtgcgtagt gctgaccatg atgctgctat ggagcagtcg cgaactgcgg
    10561 gcgcggcaaa aaatgctggc gggccaggcg ctggagatga gcggcatgtt gatcctcgcc
    10621 atgccgggga ttgtgctggc aacgggcttc tttttactgc tcaacagcac cattggcctg
    10681 ccgcaatctg ctgacggcat tgtgattttc accaatgcgt taatggcgat cccctacgcc
    10741 ctgaaagtac tggaaaaccc gatgcgcgat atcaccgccc gctacagcat gttgtgtcag
    10801 tcgctgggga ttgaaggctg gtcgcgcttg aaagtggtgg aactgcgcgc tctgaaacgt
    10861 ccactggcgc aggcgctggc ctttgcctgc gtgctgtcga ttggtgattt tggcgtggtg
    10921 gcgttgttcg gtaacgatga tttccgcacc ctgccgtttt atctctacca gcaaattggc
    10981 tcctatcgca gccaagacgg cgcggtcacc gcgttaattc tgctgctact ctgttttctg
    11041 ctgtttaccg tgattgaaaa aataccgggg cgaaatgtta aaactgactg atatcacctg
    11101 gctttaccaa catttgccga tgcgttttag cttaacggtg gaacgcggcg agcaggtggc
    11161 gatcctcggg ccaagcggcg cgggtaaaag taccctgctg aatttgatcg ccggttttct
    11221 gacgccagcc agcggttcgc tgactatcga tggcgttgat cacaccacga cgccgccgtc
    11281 acgccgtccg gtgtcgatgc tgtttcagga gaacaacctg ttcagccacc tgacggtcgc
    11341 gcagaacatc gggctggggc taaatccggg attgaaactg aacgcggcac agcaggagaa
    11401 aatgcacgcg attgcccgtc agatggggat tgataattta atggcgcggt taccgggcga
    11461 gctttccggc ggtcagcgac agcgcgtggc gttagcgcgt tgtctggtac gcgaacagcc
    11521 gattttattg ctcgatgaac cgttctctgc gctcgatccg gcgttacgtc aggagatgtt
    11581 gacgctggtg agcacgagct gccagcagca aaaaatgacg ttattgatgg tgtcgcacag
    11641 cgtggaagat gcggcgcgga tcgccacgcg ctcggtggtg gtcgccgacg ggcgcatcgc
    11701 ctggcagggt aaaaccgatg agttgttaag cggtaaggcg agtgcttcgg cgctgttggg
    11761 aattacaggt tagtatgcac tgtcggatgc ggcgtgaacg ccttatccga cctacgggta
    11821 gtgcgttttg taggcctgat aagacgcggc aagcgtcgca tcaggcaacc cgcacaagac
    11881 tatctcctaa accccaacca ctttacgcaa aatatcgata tacaccggca tcaacggatg
    11941 gcgaactaac accaccagcg ccaccacgcc gatggcagaa atcaacggcg tcagccacaa
    12001 caaacgaccg cgggacaaat aatgactcaa acggtcagtc gctttaccac tgcgccataa
    12061 ccgccagcac agccagccac caacccacaa aaataccgcc gtcgccagca gcaaccattt
    12121 aaactcaccg ctctgcattc cggcaggaat atcgatcgcc gcgcccgcca gaatccctgg
    12181 caggaagtaa aacggcggcc acagcaggca gccgataata ttcggcgtaa taaatttagc
    12241 caccggcaga tccagcattc ccgccaccat tggcaccagc ggacgcgtcg ggccaacaaa
    12301 acgaccgacc agaatggtga acatgctgtg ttgatgcaac gcatgttcag ttttatcgag
    12361 taatgctttg ttcttcttca gaaatgacca gcgatgcaat ggctttttaa aacgccagcc
    12421 cagccagaag gagatccagt cacccagcag acaaccgacg atccctgcca gccaggcgtg
    12481 ccagaaactt aactcgccgc tgccaatcag cgctcccagc cccgccatca gcaccgtacc
    12541 gggtagaatc aaaccgacca gcgccagcga ctccagaaag gccaccaaca ccaccgccat
    12601 caatgaatac acggtggatt gggtaataaa gtgttccagc aatgcttgca taatgtgcct
    12661 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg
    12721 tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac
    12781 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatactcgc gagaaataga
    12841 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
    12901 aaagcagctt cgcctggctg atgcgttggt cctcgcgcca gcttaagacg ctaatcccta
    12961 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
    13021 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
    13081 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
    13141 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
    13201 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
    13261 aacccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
    13321 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
    13381 tctctcctgg cgggaacagc aaaatatcac ccggtcggca gacaaattct cgtccctgat
    13441 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
    13501 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
    13561 gatgggcgtt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
    13621 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga tattgccgtc
    13681 actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt attaaagcat
    13741 tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg tctataatca
    13801 cggcagaaaa gtccacattg ataatttgca cggcgtcaca ctttgctatg ccatagcatt
    13861 tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct ctactgtttc
    13921 tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc ctcgattttg
    13981 gcagtgattc tgtgcgagct ttggcggtgg actgcaccac cggtgaagaa atcgccacca
    14041 gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg aataaccagt
    14101 tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa accgtgcttg
    14161 cagaacttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac agtaccggct
    14221 cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg gagtttgccg
    14281 aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa gaagcggaag
    14341 agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctat attggcggta
    14401 tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag gacagcgccg
    14461 tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct ctgctttccg
    14521 gtaccacgcg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat aagtcgctat
    14581 ggcacgaaag ctggggcggc ctgccgccag ccagtttctt tgatgagctg gacccgatcc
    14641 tcaatcgcca tttgccttcc ccgctgttca ctgatacctg gactgccgat attccggtgg
    14701 gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg gtgatttccg
    14761 gcggcgcgtt tgactgccat atgggcgcag ttggtgcagg cgcacagcct aacgcactgg
    14821 taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag agcgttggcg
    14881 agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag cgtggtgcct ggatttatcg
    14941 gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggttcggt cgcgtactcg
    15001 gctggccgct ggaacagctt gccgcccagc atccggaact aaaagagcaa atcaacgcca
    15061 gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg tctctggatc
    15121 acctgccggt ggtgctcgac tggtttaacg gccgccgcac accgaacgct aaccaacgcc
    15181 tgaaaggggt gattaccgat cttaacctcg ctaccgacgc tccgctgctg ttcggcggtt
    15241 tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc gatcagggga
    15301 tcgccgttaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag gtcattatgc
    15361 aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac cagtgctgtg
    15421 cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac atcccctcag
    15481 cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc gaacaggcac
    15541 aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc gaacaacact
    15601 atcttccaac ttccgccccg gcacaggctg cccaggccgt tccgactcta taaggacacg
    15661 ataatgacga tttttgataa ttatgaagtg tggtttgtaa ttggcagcca gcatctgtat
    15721 ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa tgcgctaaat
    15781 acggaagcga aactgccctg caaactggtg ttgaaaccgc tgggcaccac gccggatgaa
    15841 atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct ggtggtgtgg
    15901 ctgcacacct tctccccggc caaaatgtgg atcaacggcc tgaccatgct caacaaaccg
    15961 ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt gggacagtat cgatatggat
    16021 tttatgaacc tgaaccagac cgcgcacggc ggtcgcgagt tcggcttcat tggcgcgcgt
    16081 atgcgtcagc aacatgccgt ggttaccggt cactggcagg ataaacaagc ccatgagcgt
    16141 atcggctcct ggatgcgtca ggcggtctct aaacaggata cccggcatct gaaagtctgc
    16201 cgttttggcg ataacatgcg tgaagtggcg gtcaccgatg gcgataaagt tgccgcacag
    16261 atcaagttcg gtttctccgt caatacctgg gcggttggcg atctggtgca ggtggtgaac
    16321 tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg ctacaccatg
    16381 acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc ggcgcgtatt
    16441 gagctgggga tgaaacgttt cctggaacaa ggtggcttcc acgcgttcac caccaccttt
    16501 gaagatttgc acggtctgaa acagcttcct ggtctggccg tacagcgtct gatgcagcag
    16561 ggttacggct ttgcgggcga aggcgactgg aaaactgccg ccctgcttcg catcatgaag
    16621 gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac ctatcacttc
    16681 gagaaaggca atgacctggt gctcggctcc catatgctgg aagtctgccc gtcgatcgcc
    16741 gcagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa ggacgatcct
    16801 gcccgcctga tcttcaatac ccaaaccggt ccagcgattg tcgccagctt gattgatctc
    16861 ggcgatcgtt accgtctact ggttaactgc atcgacacgg tgaaaacacc gcactccctg
    16921 ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac cggatctgcc aactgcttcc
    16981 gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct tcagccatgc gctgaacctc
    17041 aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat tgataacgac
    17101 acccgcctgc cagcgtttaa agacgcgctg cgctggaacg aagtgtatta cgggtttcgc
    17161 cgctaagtag ccgcatccgg tatgtaacgc ctgatgcgac gctggcgcgt cttatcaggc
    17221 ctacacgctg caattttgta ggccggataa gcaacgcgca tccggcattc agcgcctgat
    17281 gcgacgctga cgcgtcttat ctggcctaca cgctgcgatt ttgtaggccg gataagcaac
    17341 gcgcatccgg cacgaaggag tcaacatgtt agaagatctc aaacgccagg tattagaagc
    17401 caacctggcg ctgccaaaac ataacctggt aacgctcacc tggggcaacg tcagcgccgt
    17461 tgatcgcgag cgcggcgtct ttgtgatcaa accttccggc gtcgattaca gcatcatgac
    17521 cgccgacgat atggtcgtgg tcagcatcga aaccggtgaa gtggttgaag gtacgaaaaa
    17581 gccctcctcc gacacgccaa ctcaccggct gctctatcag gcattcccgt ccattggcgg
    17641 cattgtgcac acgcactcgc gccacgccac catctgggcg caggcgggtc agtcgattcc
    17701 agcaaccggc accacccacg ccgactattt ctacggcacc attccctgca cccgcaaaat
    17761 gaccgacgca gaaatcaacg gtgaatatga gtgggaaacc ggtaacgtca tcgtagaaac
    17821 cttcgaaaaa cagggtatcg atgcagcgca aatgcccggc gtcctggtcc attctcacgg
    17881 cccatttgca tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg tgctggaaga
    17941 ggtcgcttat atggggatat tctgccgtca gttagcgccg cagttaccgg atatgcagca
    18001 aacgctgctg aataaacact atctgcgtaa gcatggcgcg aaggcatatt acgggcagta
    18061 atgactgtat aaaaccacag ccaatcaaac gaaaccaggc tataatcaag cctggttttt
    18121 tgatggaatt acagcgtggc gcaggcaggt tttatcttaa cccgacactg gcgggacacc
    18181 ccgcaaggga cagaagtctc cttctggctg gcgacggaca acgggccgtt gcaggttacg
    18241 cttgcaccgc aagagtccgt ggcctttatt cccgccgatc aggttccccg cgctcagcat
    18301 attttgcagg gtgaacaagg ctttcgcctg acaccgctgg cgttaaagga ttttcaccgc
    18361 cagccggtgt atggccttta ctgtcgcgcc catcgccagt tgatgaatta cgaaaagcgc
    18421 ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc gcccgccaga acgctatctg
    18481 atggagcggt ttatcacctc accggtgtgg gtcgagggtg atatgcacaa tggcgctatc
    18541 gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg ggtttctatt
    18601 gatatcgaaa ccacccgcca cggtgagctg tactgcatcg gcctggaagg ttgcgggcag
    18661 cgcatcgttt atatgctggg gccagagaat ggcgacgcct ccgcgctcga ttttgaactg
    18721 gaatacgtcg ccagtcgccc gcagctactg gaaaaactta acgcctggtt tgccaactac
    18781 gatcctgatg tgatcatcgg ttggaacgtg gtgcaattcg atctgcgaat gctgcaaaaa
    18841 catgccgagc gttatcgtat tccactgcgg ctggggcgcg ataacagcga gctggagtgg
    18901 cgcgagcacg gttttaaaaa cggtgtcttt tttgctcagg ctagaggtcg gctaattatc
    18961 gacggtatcg aggcgctgaa atccgcgttc tggaatttct cttcattctc gctggaaacc
    19021 gtcgcccagg aattgttggg cgaaggtaaa tctatcgata atccgtggga tcgaatggac
    19081 gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg caacttataa cctgaaagat
    19141 tgcgagctgg tgacgcagat cttccataaa actgaaatca tgccattttt actcgaacgc
    19201 gcgacggtga acggcctgcc ggtagaccgg cacggcggtt cggtggcggc gtttggtcat
    19261 ctctattttc cccggatgca tcgcgccgga tatgtcgcgc ctaatctcgg cgaagtgccg
    19321 ccgcacgcca gccctggcgg ctatgtgatg gattcgcggc cagggcttta tgattcggtg
    19381 ctggtgctgg actacaaaag cctgtacccg tcgatcatcc gcaccttttt gattgatccc
    19441 gtcgggctgg tggaaggaat ggcgcagcct gatccagagc acagcaccga aggctttctc
    19501 gatgcctggt tctcgcgaga aaaacattgc ctgccggaga ttgtgactaa catctggcac
    19561 gggcgcgatg aagccaaacg tcagggtaat aagccgcttt cgcaggcgct gaagatcatt
    19621 atgaatgcct tttatggcgt gctcggcacc accgcctgcc gcttcttcga tccgcgcctg
    19681 gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc aaaccaaagc gttgattgaa
    19741 gcgcagggct acgacgtgat ctacggcgat accgactcaa cgtttgtctg gctgaaaggc
    19801 gcacattcgg aagaagaagc ggcgaaaatc ggtcgtgcac tggtgcagca cgttaacgtc
    19861 tggtgggcgg aaacgctgca aaaacaacag ctgaccagcg cactggaact ggagtatgaa
    19921 acccatttct gccgttttct gatgccaacc attcgcggag ccgataccgg cagtaaaaag
    19981 cgttatgccg ggctgattca ggagggcgac aaacagcgga tggtgtttaa agggctggaa
    20041 accgtgcgta cggactggac gccgctggcc cagcaatttc agcaggagct ttacctgcgt
    20101 atcttccgca acgagccata tcaggagtac gtccgcgaaa ccatcgacaa actgatggcg
    20161 ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc gccgtccgct gagcgaatat
    20221 cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga aaaccaaaag
    20281 cgcggtcgcc ccttgcaata tcagaaccgc ggcactatta agtacgtatg gaccaccaac
    20341 ggcccggagc cgctggacta ccaacgttca ccactggatt atgaacacta tctgacccgc
    20401 cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt tgctacactt
    20461 atgaccgggc aacttgggct attttgagca aaaaaagagt tcgccagata ccattttgat
    20521 gcgtgacgaa tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata
    20581 acaccactac ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag
    20641 ccgaacacat atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt
    20701 gggacttgga accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac
    20761 tggtgaaaac cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc
    20821 tggtgatacc attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga
    20881 aaatggcttg ctgacctata tcggtactcg cctggatact gaagagtccg gcgtggccct
    20941 gcgtgaagtt ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc
    21001 cgggcagatt gaccgtatgg accgctttgc gctgcgttat cgtgcgcgta agtattccag
    21061 cgaacagttc cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgatcccgca
    21121 ccagctcaac atcgctcatg atgttggccg ccgccacgcg ccgcgcgtcc tgctggctga
    21181 cgaagtgggt ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactactctc
    21241 tggcgctgct gaacgtgtgc tgattatcgt cccggaaacc ttacagcatc agtggctggt
    21301 agaaatgctg cgccgtttca acctgcgctt tgccctgttt gatgatgagc gttatgccga
    21361 agctcagcac gatgcttaca acccgttcga caccgaacag ctggtgattt gctcgctgga
    21421 ttttgcccgt cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct
    21481 ggtggtcgat gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca
    21541 ggccattgaa caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga
    21601 acagctgggg atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca
    21661 cgattttgcg cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc
    21721 catgctgctg gcaggtaaca aactgagcaa tgacgaactg aacatgctcg gcgagatgat
    21781 cggcgagcag gatatcgagc cgctgttgca agcagcaaac agcgacagcg aagatgccca
    21841 gagcgcccgt caggaactgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct
    21901 gttccgtaac acccgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa
    21961 gctgccgcta ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg
    22021 taaaagtgcg gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt
    22081 tgaaggtgat aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta
    22141 cctgaccagc catcgctctc agaaagtgct ggtgatctgc gctaaagctg ccactgcgct
    22201 gcaactggag caagtactgc gcgaacgtga aggtatccgc gctgctgtgt tccacgaagg
    22261 catgtcgatt attgagcgtg accgcgctgc cgcctggttt gctgaagaag acaccggcgc
    22321 acaggtactg ctgtgctcgg aaatcggttc tgaaggacgt aacttccagt tcgccagcca
    22381 catggtaatg tttgacctgc cattcaaccc ggatctgctg gagcagcgta ttggtcgtct
    22441 ggatcgtatc ggtcaggcgc acgatattca gatccatgtg ccttatctgg aaaaaactgc
    22501 tcagtcggtg ctggtgcgct ggtatcacga aggtctggat gcattcgagc acacctgccc
    22561 gaccggacgc actatttacg atagcgtcta caacgatctg attaactatc tggcttcacc
    22621 ggatcaaacc gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct
    22681 gaaagcacaa ctggaacagg ggcgtgaccg cctgctggaa atccactcca acggtggcga
    22741 aaaagcccag gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc
    22801 cttcgccatg aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat
    22861 gatcgtgctg acgccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga
    22921 tggcatcacc atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg cacagtttat
    22981 tacctgggag catccgctga tccgcaacgg tctggatctg atcctttctg gcgatactgg
    23041 tagcagcacg atttcactgt tgaaaaacaa agcgttgccg gtaggtacgc tgttggtgga
    23101 actgatttac gtggttgaag cccaggctcc gaagcagttg cagctcaacc gcttcctgcc
    23161 accgacgccg gtacgtatgc tgctggataa aaacggcaac aacctggcgg cgcaggtaga
    23221 gtttgaaacc tttaaccgtc aacttaacgc ggttaaccgt cacaccggca gcaaactggt
    23281 taacgccgtg cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa
    23341 atctgcccgt gcattgattg atgccgcacg taacgaagcc gacgaaaaac tgtctgccga
    23401 gctgtctcgt ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg acgaactgac
    23461 cgccattgag agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gctggcgtct
    23521 ggatgccctg cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa
    23581 aactacaatc caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg
    23641 gtggtcaaca agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagac
    23701 agcgtgatga cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat
    23761 atggctacta gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa
    23821 cgccagttcc gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca
    23881 tcccctgcgg aaggtctggt agatctgccg ctgatttgcg actggccaaa ccgcccgaaa
    23941 cagaaagtct gttacgaaac aggtaaacct gcgcagacgg aatatgaagt ggtggagtat
    24001 gcggcggata acacggcaag agtggtgtta aaaccgatta ccggtcgttc gcatcaattg
    24061 cgtgtgcata tgctggcgct gggtcatccg attctcggcg atcgttttta tgcatcaccg
    24121 gaagcgagag cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat
    24181 ccggcgtatg gcaatagtat gacgtttaaa gcgccagcgg atttttaacc tttcctgtac
    24241 gtttataccc tcatcctaag ggctgcctct tgtacataaa tccccgagtc aggggatttt
    24301 ttgtttctgc caaactgctt tcagttgttg cgtgtggcca cgctttccat tcggtaattc
    24361 ccttcctgct ccacagcgaa tttttgaact ttttcttcac ccgttgatcg tctctactat
    24421 ttcattttat cgggtctgtt atggtccttt ctgattgtta ttcatgggct aattagcagt
    24481 ctggttggcg atgcccatct cggtgatccg ggtcgtgcac gacgccttgc gtcacgagcc
    24541 tcttctctcg ctcatcatgc aggtctttct aattgtgaaa tcatcccacg ctactgctaa
    24601 ggttgaaggg gcgtcccagc cccttcaacc ttagcttatt ctggatcatt caataattgc
    24661 gttggcaata atttcttcag cagtactttg tttagtagca atttcttcgg tagcaatttt
    24721 tttgggagtg attttattaa taataattac ctcatcatat aggtgggtag caccgtccac
    24781 ggtagcaccg tccaggatag aaccgtccag ggtagcaccg tccaggttag cattccaccc
    24841 aggttccaag taaagtaatt tattgtgacc tttcattgtt ttgagaccaa ggtaacaatc
    24901 cctcatgtca acgccgggaa ggtaagcctc tccaagatca acgcccaagt tacctgggcc
    24961 tttatgcagc tgcaacgtct gagtacatat ctccttattt tcaataaaaa aagtgacact
    25021 taactcgata tcaccgaagg tctgggcgac ccagctcata ctgccctttg cgaccatctc
    25081 atccggattt tttgcgcatg ttttgagttc attgaataat tccaggctat tgataaactt
    25141 ttctttctca ttctcgttga cagccccagt aaagtatttt gaggcgttga tgttgccagc
    25201 ctttttattc acgaaattga acagaagttt ataagcctca gtctgtttat ctgaccagaa
    25261 cagatgacgt aacgtctccc acaagcgttg aaagatattc atcttcacgt caatcttgtc
    25321 cccgctcacc atcttataaa tatcgttatt gctcatattt aaaagcgtag ccggcatgcc
    25381 tggaactgat actttcatgg taatagcctc gcattgagtt aaaaactcat gatgcgcgct
    25441 ctgaataaat atactttcaa aaatcgctca ttcagatatg tgttttttat ttttaagacg
    25501 taaaaatata attccgctcg tcgtaaagct ctcaacctta agcagggtca acgcgataaa
    25561 accaacagaa accctcccgt aaaccgtgta ttgagcctgt ccaccactct gagtaaacgc
    25621 cttttctcaa agaagtgacc gccagggcgg ccacctttaa tcgtgggcag ttactcagca
    25681 tcatttacag ggtcacttaa accctttctg ctgcttaatc aattcatagg cctgctgaat
    25741 ttcctgcgct ttttgcttcg ccatctccat catctcaggc ggcaaacctt tcgccaccaa
    25801 cttatcggga tggtgttcac tcatcagctt acggtaggca cgtttgatgg tggtcgcatc
    25861 atccgtcggc ttcacgccca aaacattaca ggcatcttcc agcgtcggac cacgctgcgc
    25921 ttgctgccag ttaccaccgc cagattgctg ctgataaccg ccgccaaact gtgcaccgcc
    25981 ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag atccctaatt cttcagcaat
    26041 gacatacagc accgcccgtt cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat
    26101 ctggatctcc agaaacatac gaattaagtc aaaacgacca aagcagacac tgcgaaactg
    26161 gcgcatcttt tcgcgcagcg ggtaattgtc tgattttccc acccggaacg cgttttgcgc
    26221 cgcagtacgg gaagcgccat gaaggttcat tcggtccatc aactggctgg cgatatgaat
    26281 atcagcctcc gtgacgcgac ctttggattt ggttaaatgc cccatcactt caaaagtggt
    26341 ggcaaaaaac agcgcctgac gctcacgctg gttggcaaac cacgccattt tacggctacg
    26401 ggctttatca aacatatggc caattaacag gcctaacact acgccccaaa agccgccgcc
    26461 catcagtaag gccacggcca cgccaatgat ttttccccaa tactgcatat attccccaaa
    26521 tcgacacacg gatatcaggg ctatctccca caatataaag gtgcttttac cgttttccgg
    26581 ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat catacctgtc attcaatgcc
    26641 gtgcctaaca ccactgacgc tattcggaca ggattaacac tagcgtagag atgacgagta
    26701 cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac
    26761 gtatgaaaaa acgtatcccc actctcctgg ccaccatgat tgccaccgcc ctttatagtc
    26821 aacagggact ggcagccgac ctcgcctcac agtgcatgtt gggcgtgcca agttatgacc
    26881 gtcctctggt acagggcgat accaatgact tacccgtgac tatcaatgct gaccacgcga
    26941 aaggggacta cccggatgac gccgtgttta ctggcagcgt ggatatcatg cagggtaaca
    27001 gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga ggcaccagga caaccggagc
    27061 cggtacgtac cgttgatgcg ctcggtaatg tccattacga cgataaccag gtgatcctca
    27121 aagggccgaa aggctgggcg aatctgaaca ccaaagatac caacgtctgg gaaggtgatt
    27181 accagatggt gggtcgccag ggccgcggta aagcggacct gatgaaacaa cgtggcgaaa
    27241 accgctatac cattctggat aacggtagct ttacctcctg tctgccgggt tctgacacct
    27301 ggagcgtggt aggtagcgaa attattcatg accgcgaaga acaagttgcg gagatctgga
    27361 acgcccgctt taaagttggc ccggtaccga tcttttatag cccctatttg cagttgccgg
    27421 tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc caagtacacc accaccaact
    27481 actttgagtt ctacctgcca tattactgga acatcgcgcc aaatatggat gccaccatca
    27541 cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga gaacgaattc cgctacctct
    27601 cccaggcggg cgctggcttg atggaactgg actatctgcc ttcagataaa gtctatgaag
    27661 atgaacaccc gaacgatgac agttcacgtc gttggttatt ctactggaac cactccgggg
    27721 tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa ggtcagcgat cctagctact
    27781 tcaatgattt cgataacaag tacggttcca gtactgacgg ctacgcaacg caaaaattca
    27841 gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc aaccaagcag ttccaggtct
    27901 tcagcgaaca gaacaccagt agctactcgg cagagccgca gttagacgtt aactactacc
    27961 agaatgatgt tggtccgttt gatacgcgta tttacggcca ggcagtgcac tttgttaaca
    28021 ccagagacga catgcctgaa gcaactcgtg ttcacctgga accgaccatc aatttgccgc
    28081 tctctaataa ctggggcagc atcaataccg aagcgaagtt gctggcaacc cattatcagc
    28141 aaaccaatct tgactggtat aacagcaatc cacaaaacaa taagttggca gattctgtta
    28201 accgtgtaat gccgcaattt aaagttgacg gtaaaatggt cttcgaacgt gatatgaatc
    28261 tgctggcacc gggctttacc caaacgctgg aaccgcgcgt gcagtatttg tacgtgccgt
    28321 atcgcgatca aagcgatatc tacaactacg actctgcctt attgcaatcc gattattcgg
    28381 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
    28441 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttctg
    28501 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
    28561 atgacgacaa aacgggctca ctggtgtggg caggcgacac ctactggcgt atctccgagc
    28621 gttggggatt gcgtggcggg attcagtacg acactcgcct ggataatgtg gcgaccagta
    28681 gttctagtat tgagtatcgc cgagatgaag accgtctggt gcagctgaac taccgttacg
    28741 ccagcccgga atatattcag gctacactgc caagagacag tagagataaa cgttggaatg
    28801 cccctcagta caaagatggt atttcgcagg taggtgctgt cgccagctgg ccaattgccg
    28861 atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag caagctgact
    28921 ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga
    28981 agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta
    29041 acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt
    29101 cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt
    29161 gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc
    29221 atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc
    29281 aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac
    29341 gctgctcagg caaggcagca acttcctgat gacgcgacgc tgcgccacca aatcatggaa
    29401 cgtttgatca tggatcaaat tatcctgcag atggggcaga aaatgggagt gaaaatctcc
    29461 gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat
    29521 cagatgcgca gccgtctggc ctacgatggt ctgaactaca acacctatcg taaccagatc
    29581 cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc
    29641 ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact
    29701 gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg
    29761 aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc
    29821 ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc
    29881 tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa
    29941 ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac
    30001 ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg
    30061 aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggaaca gattgctgct
    30121 gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca
    30181 ggttctgcta accagggcgg cgatctcggc tgggctacac cagatatttt cgatccagcc
    30241 ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca
    30301 ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg
    30361 cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc
    30421 tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta atggttaaaa
    30481 cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc
    30541 agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta
    30601 ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cacttattcc cccaactctc
    30661 ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg
    30721 tcactgcggg gcagttagcg gttgaaaatg ggcattatgt ggtggaaacg ctggcgcgag
    30781 cgtgcgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag
    30841 gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc gaagagcgtt
    30901 cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa
    30961 cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcgctt ttgcacgaag
    31021 tgattgctat tttgcatcac gatttgcgga ccaaatttgg tattgccgaa ccgcgcattc
    31081 tggtctgcgg gctgaatcca cacgcgggcg aaggcggtca tatgggtacg gaagagatag
    31141 acaccattat tccggtgctc gacgagttgc gggcgcaggg gatgaaactt aacgggccgc
    31201 tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga
    31261 tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca
    31321 ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg
    31381 cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa
    31441 tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt
    31501 cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc
    31561 gcaaaagggc caggcgatgg tcgaaatcgg ccccggtctg gcggcattga ccgaaccggt
    31621 cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatcttg cggcacgtct
    31681 gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt
    31741 taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc
    31801 ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga
    31861 catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa
    31921 ggcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc cagtgctgga
    31981 agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt
    32041 tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac
    32101 cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt
    32161 cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc
    32221 gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg
    32281 atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct
    32341 tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga
    32401 gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc
    32461 gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac
    32521 cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa
    32581 atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt
    32641 cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt
    32701 tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa agataccctc
    32761 tggctgacag gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg
    32821 aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg
    32881 gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg
    32941 ccggatgccg acgagctgct taactggctg cggcgccagc ctctgctgca aatcgacgaa
    33001 gagaaaaagc tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc
    33061 aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctc
    33121 gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga
    33181 ctgcggttta tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg
    33241 gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg
    33301 attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa
    33361 ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca
    33421 ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataaa
    33481 gatatgggtg agggagaggc ggtcgcgtct taaatcccgt cgagcataaa atctaatgct
    33541 cctttaatca acgttcggtg cgcgctggca tcgcaaaatt tcgttcccag cgcgttaact
    33601 ggaacagttg cagtctcatg cgtcatgagc acatattctt tgccgtctac cagtaaaagg
    33661 attgggttaa gccgttctgg tgggcgaata cggataaatc gttcaatagg tgttaatgga
    33721 ataacaatac ggcgattaat cacaccaata atgtcgctgg taacatcgat aacaaaggga
    33781 aacgcggcgt tcctgctgcg actgcggtat accgtaaatt gcatcgctag aagctccggt
    33841 actcatcaga gaaacatccg gtttcatcag ccaattgatt taacgcagcg atagcttctg
    33901 cgttctcttc acgccaacgt gttgctgcat gttttttaag ttcagcattg agcgcaaccg
    33961 taagggtggc gctcatattt aagcctgcat cacgagcgcg attgactaac tcacggtcga
    34021 cggtgacggt cacactttgt gtggtacgtt ttgcagtcat gatatcgtgc tcctgtacaa
    34081 tttatacgtc tagtatacac ggatatctgc acgtatcagt gatgccggaa ttctaatata
    34141 caaaattacc gccgctccag aatctcaaag caatagctgt gagagttctg cgcatcggcg
    34201 tcgtggaatt cgctgaatac cgattcccag tcatctggct cgtaatccgg gaaatgggtg
    34261 tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tttgcgcttt tggcaggaac
    34321 tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc acacgccgcg
    34381 atggcttcat ccaccgattt cacccacgtt acgcgatcgt ccgtacccgg ttgactgctg
    34441 aggataatat ttttgcgtcc tggcaacgga cggccgatgg attcccaggt atggcggccc
    34501 ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc aggcaggttc
    34561 cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc aatcagactg
    34621 atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg taaaccgtcg
    34681 tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga gtccacgctc
    34741 tccctctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg taaagtgtga
    34801 tttacgtcac tctttattag gatgagggtt tcgtttccgg ttcatccgcc atgttgccgg
    34861 tatgtttacc ttcttctgtt ccctgccagc catgtcgttg aattaatgac agatgttcgc
    34921 ggtcctcggt gatgatctca cttaacatcg cgctggtgcg tttatagacc gccgcgcggg
    34981 ctttggtgtc attctcaacc attgccatct cttccaccat ctgaatatta aagcggcgga
    35041 acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc agcgccagac
    35101 gcccggtttt cagcgcacct tcaaaggttt cacgctccgg cttttcaacg cctgcctgac
    35161 gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa tgcgggaaat
    35221 gttctttcac catctccgtc agttgcaggt tggtttgcgg atcgtcaata gcgttaatca
    35281 gcacttccgc tttcgctgct ccggcagatt ccagtaaatc catccgcgtg gcatcgccgt
    35341 aaaacacttt cataccaaat ttacgcagcg tttcgatatg gtccggatcg tgatcgagca
    35401 ccaccatttt caccccgctg gagagcagta aacgtccggt gatctgccca aaacgaccga
    35461 atccggcaat gattacccgc ggctgttctt cgtcgatctc atcggcttca cgcgcctctt
    35521 cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt gctgccatcg
    35581 acagcgccac cgccagggtc agcgatttcg cccactccgg ctccagcaca ttcgccatct
    35641 gcgccgcgcc aaataccaca aaggcaaact cactgccctg ccctaacaac accgcaaacc
    35701 agcgacgctg tttatttggc acctgcaacg gtcgggcaat cagccacagc atggcgattt
    35761 tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa tgggttttca agcagcgtgc
    35821 caaaatctat cgacatgcca acaccgatga aaaacagccc caacagcaaa cctttaaacg
    35881 gttcgatatc gctttccagc gcatgacggt attccgagct tgccagcagt acgcccgcca
    35941 gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca aagccaaaca
    36001 caaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggca acaaaacgca
    36061 gcgccggacg cgtaacatag cgccccagca ataccaccag taccagcgca cccgccactt
    36121 ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc actgctcgcc gccagtagcg
    36181 ggatcatcgc caccagcggg attgccgcga tatcctggaa cagcagcacc gcaaaggcac
    36241 tgcggcccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg gcaatcgccg
    36301 ttgatgagag cgccagcgtc ataccgatca attccgcgac ctgccagcgc agcccaagta
    36361 acatacagaa cagccccagc aggccaccgc aaatcaccat ctgcaatgcg ccaccgccga
    36421 acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg ataataaaca
    36481 gcatcagcac caccccaatc tcggcaaagt gcagaataga ttcggcatcg gtcaccagtc
    36541 gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagtacc gatcccagac
    36601 caagacgtac cgcaatgggt acaatcagcg ccgccgatcc gaggtagatc agcgcctgaa
    36661 ccagcgtatg gctatccatg atgtgcctcc tgccattcca gcagacgttg cttatagtga
    36721 cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat ggcaaacggt
    36781 ggcagccagt tcagcccgca gtagactgcc gtcgcctgta gcggctgcga cagcacatca
    36841 aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt caccgcccac
    36901 agcaaatgtt tgccatgcag cgccgtgccg ccgtgaccgt aagcccagcc gtgcgagaag
    36961 actttatcga tccaaagttt aaggagcgga ggaatgctgt accactgcat cgggtgctgc
    37021 cagacgatca gatcggcgcg agacagcgcc tcctgctcgg cggcaatatc gatattgaag
    37081 tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct tgcctgttca
    37141 agcatccgtt tattcgcatg ggaatgatgc ggatacggat gcgcataaat tatgaggatc
    37201 atagttatta gcccgcgtgt ttacgccttt gttttcccac aaagtgtagt catatattca
    37261 cgcggcttat agtcaataat attgattcag aaaagagaga aatctgaaca aattatccca
    37321 ttctctgccc gcggatcgtc atattgctgg tctgcgccag tgacattcct cgagtctccg
    37381 gggcaaacgc tacggaaatc aacaagccaa atagcgagat acccgccccc attagcatcg
    37441 tgttactgat accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc
    37501 caatacgact taaggacata atcacgccca cggcagaggc gcggatatcc gtcgggaaga
    37561 gttcattagg atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg
    37621 caaaggccat cactaccagc cagatcccca tatccgggat tagccccaaa accgccagcg
    37681 ccagcgtcat catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttagcc
    37741 acagcatcgg cggaatacag ccgagcataa agaacaggct aatcaccaca ttccctagtg
    37801 ccgcgttttt gccaaccccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa
    37861 tggcgaacat tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa
    37921 aatggcggcg attaaacaga tcgcgaaaac gggtttgctg cggctgctct tcatcgaaag
    37981 ccaccggttc gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc
    38041 gtaataacca gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg
    38101 ggatcgctgc gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc
    38161 cgaccagatc ggcacaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa
    38221 acgcccgctg acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg
    38281 caccgatgac aatgccgatg agtacccgca tcaccaacag ttcgacgggg gatgaaacaa
    38341 acatcgtcgc caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc
    38401 gtccgacttt atcggagata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg
    38461 ttcccgcgcc cagcaagcca atccagtcag cgtccagttt cagcgccgga gtcagttgct
    38521 ccagcgccac gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc
    38581 acagcaaaat gcggcggtga atagaggaga atttgagatc gtcaaagttt ctggacggtt
    38641 gcatggatta ttcctgtttt ttgcaatgag agtcagtacg gcgttacgta tcaaaccaac
    38701 atcagccgta acggaactcc acaccaaagg tgccgcgcgg gtattcccac tgttccagcg
    38761 ccgaccccag ccccagaatg cgacaggtgc cgcactccag acatccggcg taatcgaagc
    38821 gcacactgcc gtcatcctgc ttcttgtaca gacctgcggg gcacgctttc accagcagct
    38881 ccagggcctg tttatcggca tcggccttca caacaatgtg tggatgctct tcatcgacat
    38941 tgaatttatt gacgcccagt ttgacgtcca cattgacggg agaagtcata aaacggtcac
    39001 tcctttcatg ccatccttga tcagattgat gaagcccact ttcttgccgt ggcggaggat
    39061 tttcttgcgc atcagttccg gcgcactgcc gtcaatggtg aacaggtcac gcgccacgcc
    39121 caccgccagt tccgggtagc cgctaaacat gcgtgggtta tcaaggaacg ccggtagttt
    39181 ctggtacatg cgcatatcgc gcagcgggcc actctccaga tgctgacgat attccgcgag
    39241 tttttgctta ctgaaatcgt cgcttttcat cgctgaaagc acggtttttg ctgcggcttc
    39301 cccggcggca atcgccagat ccataccgcg aatggtaaaa ccgaggttca tacacattcc
    39361 ggcggcatca ccggcaatca atacgccgtc accaaccaac tccggcagca tgttgatgcc
    39421 tgcttccggc actacgtgag cggaatattc caccagcttg ccacccgcga tcagcggtgc
    39481 aacggccgga tgttgtttga aatcttccag catttgcggc accgattttt tcgcgtcatg
    39541 cagatgatgc agaccacaaa ccagccccag cgacagggta ttttcattgg tataaaggaa
    39601 gccgccgccc atcaggccat cggtgggcga tcccgcaaac agacaagccg cgccctgatt
    39661 accctgcaac tgaaaacggt cttcgattac cgacttcggt aactcgatca gttccttcac
    39721 gccaaccgcc acatccgtcg gtttgacgcg tattgccatc cccaattttt cggcaaggat
    39781 ggagttcacc ccatcagcaa ggatcaccgt tttcgcttca atcacatcgc catcggcttc
    39841 tacaccgacg actttgccat cgcgctgtac gaggttatcg acgcggatcc cggtaattaa
    39901 ctgcgcgccc gcttcttcgg cctgctccat caaccaggca tcaaatttac tgcgcaaaac
    39961 ggagtaagaa cgctgggatg gcgaggtttc gtcaccgttg cagtagtcca tagtcatcgc
    40021 tgacttttcc gtcataaacg cgagtttttc atgggtgatc aggcgttcta cgggggcgga
    40081 ttcggcgaaa cctggaataa tgtgttccag actgtgggca tagagacgcc cgccggtgac
    40141 gttcttggca cctgcggaat tgccacgctc gataactaac acttgcgcac cttcgcgggc
    40201 gagcaccagt gcggcaaccg aaccggcaag ccctgcaccg acgatgatgg cgtcaaagat
    40261 atcttcggac ataagagctc cctgtcagcg gcagggccaa aatgcagccc tgccagagtg
    40321 gatcaacgcg ctaaagctgc ggtcagtgcc ggaaggatct tcacggcatc gccaacaatg
    40381 ccgtaatccg cgtactggaa gatcggcgca tttttatctt tgttgatggc gaaaatggtt
    40441 tgcgatgcat tagcaccaac catgtgctgg atctgcccgg agatccccac cgccaggtac
    40501 agttcaggtt tcagcatcag gttggagata ccgacatagc gttcgtgctc catccatttt
    40561 tcgttttccg ccaccggacg agaacaggcc aactccgcac ctatcgcctt gcaaagctgt
    40621 tctgccagcg caatgttctc tttgcttcca atgccacgac cgacgctgac caccagacgg
    40681 gctttgtcga gatcgacgct gttgctctgg cgtgcctgag ttgccgtgcg ggtaatcgcc
    40741 acagccggag cctgccactc cacggtgtgc gtttcgccag tgcgtgacgc gtctggctga
    40801 gccgcatcga acgtgccact gctgatggtc agtaccgcat acggcgtggc aatgcgttct
    40861 tcgccaatcg ccagaccgcc gtaaaccatg tgtttcactg tcgctttacc gtcctgtacg
    40921 ctgacggtgc tggcatcgtt agacaccgcc gctttaaggc gataacccag ttttgccgcc
    40981 agtaatttgc cgcgacgggt gtttggcagc agcaccagac cgtctgcgcc gtgctggcga
    41041 atagtgtcag ccatgacacc ggcgtaatct tcgatcatcc gatcgtccgg tttgccgttt
    41101 aatttccaga catgattagc gccgagctgg attgcctgtg cgccgtcggc atcattgagg
    41161 acaaaggcat tgatttgatt agctaaagcc tgcgcaccgt tcatcagttc cggcagacga
    41221 gaaggggtat cgctgaatac ccagacttga gaaaacgtgt tcatagcatc ccctgtaatt
    41281 aaatgacttt gcgaagattt tcagcaaatg cggcgatctg ttcttcgccg tcgccttcaa
    41341 tcacgatgcg ctgacgctcg cgctgtttcg gcgcggcaac ctgttgttca gaccagacgt
    41401 ccacggcgtt aaaaccaata tccgccgccg accatacctg gactggcttt ttcgccgcgc
    41461 cgagaatggc tttcatcgaa ggaatttgtg gggagttgat atcagtggaa acagcaacaa
    41521 ccgcaggcag cggaatgctt aacgtttctg tttcatcttc cagttcgcgc tcaacggtga
    41581 gggtatctgc cgtcagggag ataattttgc tgacgccgtt aactgccgga atattgagga
    41641 tttcgcccac cagcagacca acctgctggg cataaaggtc ggaagaacca tcgccacaga
    41701 ggatcagatc aaagcctgct ttctgggcgg ctgcagccag tgcgctcgcc gtttgttgcg
    41761 gcagtgcctg ctcgaactgg tcatcaatca ccacaatcag ttcatccggg ccgcgcgata
    41821 gcacatcttt acgccctttg gcgttggtca gggctttacc gcccacactt aaggctgtca
    41881 cctgcgcctc tgctgcctgt tgctttagct ggcaagccgc ttcaatagcg ttgagatcgt
    41941 attggcttat tttggcatcg gctttgctga agtctaatga accatcagca ttattgaccg
    42001 caatatcctg ttcatcaggc acgcacttat agcaagtaat aatcttcatt gcatctccag
    42061 aaatcatgaa ggaaacgtcg ttcatatcgg tggtgttaat tacagatatc acgcccttta
    42121 atttggaaat aaaactttaa tcaccaatat tgaaaatgtc acacgcagat aaatccaact
    42181 ttcaatattg ttaagttcct caccaatatt gaaaacatgg cgtagcaaaa agaaattttc
    42241 aatattgttt tatagatcac caatattgaa agtc
//
LOCUS       DAPGRN010000030        40513 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPGRN010000030 DAPGRN010000000
VERSION     DAPGRN010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.030"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            454..2739
                     /gene="nrdA"
                     /locus_tag="RHW33_001746"
     CDS             454..2739
                     /gene="nrdA"
                     /locus_tag="RHW33_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV1645257.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2973..4103
                     /gene="nrdB"
                     /locus_tag="RHW33_001747"
     CDS             2973..4103
                     /gene="nrdB"
                     /locus_tag="RHW33_001747"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV1645258.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            4103..4357
                     /gene="yfaE"
                     /locus_tag="RHW33_001748"
     CDS             4103..4357
                     /gene="yfaE"
                     /locus_tag="RHW33_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV1645259.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4411..5061)
                     /gene="inaA"
                     /locus_tag="RHW33_001749"
     CDS             complement(4411..5061)
                     /gene="inaA"
                     /locus_tag="RHW33_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV1645260.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVTPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5142..6332)
                     /locus_tag="RHW33_001750"
     CDS             complement(5142..6332)
                     /locus_tag="RHW33_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645261.1"
                     /translation="MKEKLWTKDFWAITIISFIIFFVFYVLLTLLPIYISDRLHASPD
                     KAGLLVTLFLIAAIVIRPFAGQWVGKYSNKTILVLSSLAFLVVTALYPFCHSIESLLF
                     IRVLHGITFGVITTVKGTISARLIPASRRGEGISFFSLAMGLAMVVGPWIGLNMARWE
                     AFNMAFWLCTGVAAVGIILSLIMTVPPVISHADGSKPKMGFAAMFDRAALPFAMVTFF
                     MTFSYAGVSAFLALYARELNLMSAASNFLLCYAIFLMICRTFTGNVCDKKGPKYVVYP
                     CLLFFTVGLVVLGYTQGSVMMVVSGALIGIGYGSVTPVFQTQIISSVEPHKIGVANSL
                     FFNAMDAGLALGACVMGMMVAHTGYRMIYLLGALLVVVAGGVYALQMKGKSGVALVVA
                     KEIH"
     gene            6497..7375
                     /locus_tag="RHW33_001751"
     CDS             6497..7375
                     /locus_tag="RHW33_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1645262.1"
                     /translation="MTLTQIHTLLAVLDYGGFTEASKRLFMTQSAVSQAIATLEQELG
                     VEILIRERRKALQLTDAGSRIASHLRAITREVNAVKEIAEQEKHNPTRTLRIGCFPSA
                     CACILPGIIRYFETYHPQIKIIPYEENSSEIIDSVQNETIDAGFVHFPVTGMYSVPVY
                     QDKFTVVLPDAHPLAKNNAISVEELVGEPLIISKGRYELSIMALFKEKNITPVIKYEF
                     NHPDTAISFIRQGLGIALLPELTLKAVAGELCSVPLEPTFYRQISLLVKEAPVEGSPL
                     FLLQMCMETLVVKGIL"
     gene            complement(7420..8499)
                     /gene="glpQ"
                     /locus_tag="RHW33_001752"
     CDS             complement(7420..8499)
                     /gene="glpQ"
                     /locus_tag="RHW33_001752"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV1645263.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLHKDN"
     gene            complement(8504..9862)
                     /gene="glpT"
                     /locus_tag="RHW33_001753"
     CDS             complement(8504..9862)
                     /gene="glpT"
                     /locus_tag="RHW33_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV1645264.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYRNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHQELLQKRN
                     GG"
     gene            10135..11763
                     /gene="glpA"
                     /locus_tag="RHW33_001754"
     CDS             10135..11763
                     /gene="glpA"
                     /locus_tag="RHW33_001754"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV1645265.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11753..13012
                     /gene="glpB"
                     /locus_tag="RHW33_001755"
     CDS             11753..13012
                     /gene="glpB"
                     /locus_tag="RHW33_001755"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV1645266.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQAVTDIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELGLAVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            13009..14199
                     /gene="glpC"
                     /locus_tag="RHW33_001756"
     CDS             13009..14199
                     /gene="glpC"
                     /locus_tag="RHW33_001756"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV1645267.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            14392..15387
                     /locus_tag="RHW33_001757"
     CDS             14392..15387
                     /locus_tag="RHW33_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV1645268.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPKLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CDDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     QGKLEGLQKGKLEGLQEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCH"
     gene            15388..15597
                     /locus_tag="RHW33_001758"
                     /pseudo
     CDS             15388..15597
                     /locus_tag="RHW33_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15638..16441)
                     /gene="yfaU"
                     /locus_tag="RHW33_001759"
     CDS             complement(15638..16441)
                     /gene="yfaU"
                     /locus_tag="RHW33_001759"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HDV1645269.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVDGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(16459..17748)
                     /locus_tag="RHW33_001760"
     CDS             complement(16459..17748)
                     /locus_tag="RHW33_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645270.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQIAALLGTKVGFT
                     ASVVTAIPWIAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(17805..19010)
                     /gene="rhmD"
                     /locus_tag="RHW33_001761"
     CDS             complement(17805..19010)
                     /gene="rhmD"
                     /locus_tag="RHW33_001761"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HDV1645271.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKLVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(19025..19807)
                     /locus_tag="RHW33_001762"
     CDS             complement(19025..19807)
                     /locus_tag="RHW33_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDV1645272.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCNAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTKLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPATVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(20027..21229)
                     /locus_tag="RHW33_001763"
     CDS             complement(20027..21229)
                     /locus_tag="RHW33_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV1645273.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(21329..21871)
                     /locus_tag="RHW33_001764"
     CDS             complement(21329..21871)
                     /locus_tag="RHW33_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645274.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            22150..22575
                     /gene="nudI"
                     /locus_tag="RHW33_001765"
     CDS             22150..22575
                     /gene="nudI"
                     /locus_tag="RHW33_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV1645275.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(22614..23216)
                     /locus_tag="RHW33_001766"
     CDS             complement(22614..23216)
                     /locus_tag="RHW33_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV1645276.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            23524..24663
                     /gene="arnB"
                     /locus_tag="RHW33_001767"
     CDS             23524..24663
                     /gene="arnB"
                     /locus_tag="RHW33_001767"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV1645277.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNHALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLQGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENIARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            24667..25635
                     /gene="arnC"
                     /locus_tag="RHW33_001768"
     CDS             24667..25635
                     /gene="arnC"
                     /locus_tag="RHW33_001768"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV1645278.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            25635..27617
                     /gene="arnA"
                     /locus_tag="RHW33_001769"
     CDS             25635..27617
                     /gene="arnA"
                     /locus_tag="RHW33_001769"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV1645279.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFGYAGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQASDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            27614..28504
                     /locus_tag="RHW33_001770"
     CDS             27614..28504
                     /locus_tag="RHW33_001770"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV1645280.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            28504..30156
                     /gene="arnT"
                     /locus_tag="RHW33_001771"
     CDS             28504..30156
                     /gene="arnT"
                     /locus_tag="RHW33_001771"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV1645281.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLTIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            30153..30488
                     /gene="arnE"
                     /locus_tag="RHW33_001772"
     CDS             30153..30488
                     /gene="arnE"
                     /locus_tag="RHW33_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV1645282.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            30488..30874
                     /gene="arnF"
                     /locus_tag="RHW33_001773"
     CDS             30488..30874
                     /gene="arnF"
                     /locus_tag="RHW33_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV1645283.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(30868..31134)
                     /gene="pmrD"
                     /locus_tag="RHW33_001774"
     CDS             complement(30868..31134)
                     /gene="pmrD"
                     /locus_tag="RHW33_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV1645284.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(31244..32599)
                     /gene="menE"
                     /locus_tag="RHW33_001775"
     CDS             complement(31244..32599)
                     /gene="menE"
                     /locus_tag="RHW33_001775"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV1645285.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYASARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(32596..33558)
                     /gene="menC"
                     /locus_tag="RHW33_001776"
     CDS             complement(32596..33558)
                     /gene="menC"
                     /locus_tag="RHW33_001776"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV1645286.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETREEAQSVLLAWVNNWLAGDCELPQMPSVGFGVSCALAELADTLPQAA
                     NYRTAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPVVEVDALERLL"
     gene            complement(33558..34415)
                     /gene="menB"
                     /locus_tag="RHW33_001777"
     CDS             complement(33558..34415)
                     /gene="menB"
                     /locus_tag="RHW33_001777"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV1645287.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(34430..35188)
                     /gene="menH"
                     /locus_tag="RHW33_001778"
     CDS             complement(34430..35188)
                     /gene="menH"
                     /locus_tag="RHW33_001778"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV1645288.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCLEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILRFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFLALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(35185..36855)
                     /gene="menD"
                     /locus_tag="RHW33_001779"
     CDS             complement(35185..36855)
                     /gene="menD"
                     /locus_tag="RHW33_001779"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV1645289.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETVLADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(36944..38239)
                     /gene="menF"
                     /locus_tag="RHW33_001780"
     CDS             complement(36944..38239)
                     /gene="menF"
                     /locus_tag="RHW33_001780"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV1645290.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGETAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(38318..38623)
                     /gene="elaB"
                     /locus_tag="RHW33_001781"
     CDS             complement(38318..38623)
                     /gene="elaB"
                     /locus_tag="RHW33_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV1645291.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(38678..39139)
                     /locus_tag="RHW33_001782"
     CDS             complement(38678..39139)
                     /locus_tag="RHW33_001782"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1645292.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEIYEEDGIPHIGMAREVIQA"
     gene            39204..40121
                     /gene="rbn"
                     /locus_tag="RHW33_001783"
     CDS             39204..40121
                     /gene="rbn"
                     /locus_tag="RHW33_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV1645293.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            40309..>40513
                     /locus_tag="RHW33_001784"
     CDS             40309..>40513
                     /locus_tag="RHW33_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HDV1645294.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDIL"
BASE COUNT        10010 a        10870 c        10070 g         9563 t
ORIGIN      
        1 tttaacaata accgaataga aaacaaccat ttcgacatca acaatcgctt acattcgctt
       61 atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag atcaattttt
      121 gcaatcatta gcaaaaagat taataaacca tctatatcaa tttatccaac ctattctgcc
      181 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
      241 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      301 aagttatgca cttgcaagag ggccattttc acactatctt gcagtgaatc ccaaacatac
      361 cccctatata tagtgttcta agcagcttcc cgtactacag gtagtctgca cgaaactatt
      421 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      481 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      541 gcagaaggac tgcataacgt ttcgatttcc caggtcgaac tgcgctccca cattcagttt
      601 tatgacggta tcaagacctc tgacatccac gaaactatta tcaaggctgc cgcagacctg
      661 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat cttccacctg
      721 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt ggtgaaaatg
      781 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      841 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      901 caactggaag gcaaatatct ggtacagaac cgcgtgaccg gcgaaatcta cgaaagcgcc
      961 cagttccttt atattctggt tgctgcgtgc ctgttctcga actacccgcg tgaaacgcgc
     1021 ctgcaatacg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc gctgccgacg
     1081 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
     1141 tgcggcgaca gcctggactc catcaacgcc acctccagcg cgattgttaa atacgtatcc
     1201 cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag cccgattcgc
     1261 ggtggcgaag cgttccatac cggttgtatt ccgttctaca aacatttcca gacagcggtg
     1321 aaatcctgct ctcagggcgg cgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1381 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga aggcaaccgc
     1441 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg tctgctgaaa
     1501 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1561 gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga cagcatccgc
     1621 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcatctacc
     1681 ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt tgatccggcc
     1741 atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac caaaccgctg
     1801 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1861 ggcgcaatta atagcctgga tgaactggaa gagctggcaa ttctggcggt tcgtgcactt
     1921 gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg agcgatgggt
     1981 cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa gcatggtaaa
     2041 cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc gattcagtat
     2101 tacctgctga aagcgtctaa tgagctggcg aaagagcaag gcgcgtgccc gtggtttaac
     2161 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatacc
     2221 atcgctaatg agccgctgca ttacgactgg gaagctctgc gtgagtcaat caaaacgcac
     2281 ggcctgcgta actccacgct ttctgctctg atgccgtccg agacttcttc gcagatctct
     2341 aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc gtcgaaagac
     2401 ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta tgagctgctg
     2461 tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca gaaatttatc
     2521 gatcagtcga tctctgctaa caccaactac gatccgtcac gcttcccgtc aggaaaagtg
     2581 ccgatgcagc aattgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2641 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt gccgtcaatc
     2701 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2761 gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc gccagcgtcg
     2821 cctcaggctc cgggtgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2881 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2941 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     3001 aaaatgatca gctcaaagaa ccgatgtttt ttggtcagcc ggtcaacgtg gctcgctacg
     3061 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     3121 gtccggaaga agttgacgtc tcccgcgacc gtatcgatta ccaggcgctg ccggagcacg
     3181 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggtc
     3241 gtagcccgaa cgtggcgcta ttgccgctta tttctattcc ggaactggaa acctgggtag
     3301 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3361 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3421 gtgcggaagg gatctccagc tactacgatg agctgatcga aatgaccagc tactggcacc
     3481 tgctgggcga aggcacccac actgttaacg gtaaaaccgt gaccgttagc ctacgcgagc
     3541 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg atccgcttct
     3601 acgtcagctt tgcttgttcc tttgcctttg cagaacgcga actgatggaa ggcaacgcca
     3661 aaattattcg cctgattgcc cgcgacgaag ccctgcacct gaccggcacc cagcatatgc
     3721 tgaatctgct gcgcagcggc gcggacgatc ctgagatggc ggaaattgcc gaagagtgta
     3781 agcaggaatg ctatgacctg tttgtccagg cggcacaaca ggagaaagac tgggcggatt
     3841 atctgttccg tgacggttcg atgattggtc tgaacaaaga cattctctgc cagtacgtgg
     3901 agtacatcac caatatccgt atgcaggcgg ttggtttgga tctgccgttc cagacgcgct
     3961 ccaacccgat cccgtggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     4021 aggaagtgga agtcagttct tatctggtcg ggcagattga ctcggaagtg gacaccgacg
     4081 atttgagtaa cttccagctc tgatggctcg cgttaccctg cgcatcactg gcacacaact
     4141 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgtggcggt
     4201 tgagtaccag tgccgcgaag gttactgcgg ttcctgtcgc acgcgccttg ttgcaggcca
     4261 ggtggactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4321 ttgccgggca aaaggcgata ttgaaatcga gatgtgaatt ggttgtagtg ccagatacaa
     4381 cgcttatgcg tattatctgg cctacaacga ttacatggcg tagtaatacg cttttacctg
     4441 ctcccagtcc gctttgggga tcggctccag atatttttcc agctggcgga agtcatgatt
     4501 aatcgcttta tcgcgacgca agcggcgacg acttttctcc agatcaagaa aaccagcttc
     4561 tgccttacct tctgttttca catagatatg gcgaacataa caacagccat gctgacgatt
     4621 aacgctatgc attttcttaa acgccagcgc cactgctttc aacatggctt gccgtacttc
     4681 gtcagaatag ggcgttactg catgctgggc ataccagtca gcaatgctga tgaaccccgc
     4741 catatcttca gtcaccagca acgcgcgcca ttccccctca attttcaccg cttcaccaaa
     4801 aacgatcttc ggcacgatga caccggcccg ttccagttct ttaattaccg caacctcacg
     4861 gacaatcgtt ggtcggccga aaggataacg tacggaatga aacagatgat gcgtcatgcg
     4921 ctttacatat agctttttgc cgttgcgctc gacgcattgc accccgctca taccattacg
     4981 gcgatagtta ggctcttcaa cccagtcgcc ctctgttgcc caccagtgat taaattcgtc
     5041 gtgctttgct gaaactgcca taccctatcg cctgtcattt tttattaacg acaatgacta
     5101 taggtggtta cctgcgaaaa atcttaatga aacgtgtcgt attaatgaat ttcttttgcc
     5161 actactagcg cgacaccgct tttacccttc atttgcagcg catagactcc accagccact
     5221 accactaata gtgcgcccag cagataaatc attcggtagc cagtatgtgc aaccatcatc
     5281 cccatcacac aggctcccag cgccaggcct gcatccatcg cattgaagaa gagggagttt
     5341 gcgacaccga ttttatgcgg ttccactgaa ctgataatct gcgtctgaaa aactggcgtc
     5401 acggaaccat acccgatacc aatcaacgcg ccagaaacga ccatcattac gctgccctgg
     5461 gtgtagccga gaaccaccag cccaaccgta aagaacagca ggcaggggta aaccacatat
     5521 ttcgggcctt ttttgtcgca aacattgccg gtgaaggtac ggcagatcat caggaagatg
     5581 gcgtagcaga gcaggaaatt actggccgcc gacatcagat taagttcgcg ggcgtaaagc
     5641 gccagaaagg cagaaacccc ggcatacgaa aaggtcataa agaatgtaac catggcaaac
     5701 ggcaatgccg cgcgatcgaa catggcggcg aagcccatct ttggctttga accgtcggca
     5761 tggctgataa ccggcggcac ggtcataatc agcgacagga taatccccac cgccgcaacg
     5821 ccagtacata accagaaagc catattaaag gcctcccagc gcgccatatt caggccaatc
     5881 cacggcccga ccaccattgc cagccccatt gccagagaga aaaaactgat gccctcccca
     5941 cgtcgggagg ccgggatcag ccgcgcggaa atcgttccct ttaccgttgt gataaccccg
     6001 aaggtaatac catgaagcac cctaataaaa agcagtgatt ctattgagtg gcaaaaagga
     6061 tacagcgcag tgaccaccaa aaaggccaga gaagagagca ccagaatagt tttattcgaa
     6121 tatttaccca cccattgccc ggcaaagggt cgaataacaa tcgccgcaat taaaaataat
     6181 gtcaccagca gaccagcttt atcaggggat gcatggagac gatcggagat ataaataggt
     6241 aataaggtca gcaacacata aaagacgaaa aagataataa agctaataat ggtgattgcc
     6301 caaaaatctt tcgtccataa tttttctttc atactaaacg acccgttcaa atggcgcaca
     6361 aggacaccgc tcagcatcac aggctgaacc actacaggtg cgtcaaaatg tgatttactc
     6421 gatagaaata gagtataaat ggatgcactc tataagagaa attaatcgtt ttaatcatat
     6481 taattagata ttactaatga cactaaccca aatccacacc ctgctcgccg tactggatta
     6541 tggcggcttt accgaagcca gtaagcgcct gtttatgacg caatccgccg taagccaggc
     6601 cattgccacc ctggagcaag agcttggcgt agagatcctg atccgcgagc ggcgaaaagc
     6661 actgcagctg actgacgccg gctcacgcat cgcaagccat ctgcgggcga ttacccgtga
     6721 agtgaacgct gtgaaagaaa ttgccgagca ggaaaaacat aacccgacca gaacgctgcg
     6781 catcggctgc tttcccagcg cctgcgcgtg tattttaccg ggtattattc gctattttga
     6841 aacttatcat ccgcagataa aaatcattcc ttacgaagag aacagcagtg aaattattga
     6901 ttcggtgcaa aacgaaacta tcgatgccgg ttttgtccat tttccggtaa ccggaatgta
     6961 ttccgtcccg gtatatcagg ataaatttac cgtggtgctg ccagatgctc atcccctggc
     7021 aaaaaataac gctatatcgg tggaagaact ggtgggagaa ccgctcatta tcagtaaggg
     7081 gcgttatgaa ttaagcatta tggcgttatt taaggaaaag aatattacgc cggtcattaa
     7141 atatgaattt aaccatccag atacggcaat aagttttatt cgccagggat taggaattgc
     7201 tctgttaccg gagctgacgc tgaaagccgt cgccggtgag ttatgctctg tcccgctgga
     7261 gccgacgttt tatcggcaaa tttcattgct ggtcaaagag gcgccggtag aaggcagccc
     7321 attgttttta ttgcaaatgt gtatggaaac cctggtggtc aaaggcatat tgtgaaaagg
     7381 gcatccggtc aggttttgcc ttcccggatg cctgtgtcat taattatctt tatgcaggaa
     7441 tttaaccgct ttatcaggga aatcagtaaa caacccattt acacccgctt tgttatacag
     7501 agcatcatat aactgattca catcagttgt gtattcaggc agtttatctg accgcacggt
     7561 ataaggatgc actaccagct tgttctgctg agcatcttgc accatgccag tgagtttgat
     7621 attacccggc tgcgatgtct cctcaatcaa catatggtaa tccggaccaa taccatctgc
     7681 atattccgcc acctgtttca tggcacccgg cttaaacatc cagtcgtagt tgtaattaac
     7741 ccagcttccg tccggctgtt tctgctgcgt ttcattccag tcggtatagg caatcagctg
     7801 taccagattg agatccatgc ccattttggg ttccagctca ttcttaatac gcttcagctc
     7861 atcagcatca aaacattgca aataaacttt atcgtcttta ccggtgtaac catatttctt
     7921 cagcacttcc agcgtttttg ccgcaatatc cttcccttcc tgatgatgga accacggcgc
     7981 tttgatttct ggatagatac cgatattttt cccggtagag tgatttaacc cctgaacaaa
     8041 ttcaatctct tcttcaaagg tgtgcacccg gaagtcggac ttacccattg ggaaacgccc
     8101 tggataagtc tgcacttttt taccgttttc aatatcgaaa ccttcggtaa atttcaacga
     8161 cttaatttca tccagcgtga aatctatcgc gtagtaacga ccgtctttgc gcgcccgatc
     8221 cgggaaacga tcggcaacat cagtaacacg atcgaggtaa tggtcatgca gaacaaccag
     8281 atggtcgtct ttggtcatca ccaaatcctg ttccagataa tccgctccct gcgcatacgc
     8341 catcgctttt gctggcagcg tatgctccgg caaatatcca ctggcaccgc gatgggcgat
     8401 gactattttt tcgttgctgt ccgccgccat tgcactgctt cccatgacta tagtgctcat
     8461 catgatcgcc atgctaaggt ttttcagcgt caatttcatg ccattagcct ccgttgcgtt
     8521 tttgcagtaa ttcctgatgg cgacgttttt cgccaatcat cacaacaatc aacaagataa
     8581 ccgccagaat gctgccgcca atcattacca taaagccgcc atcccagccg aagaagtcca
     8641 cggtgtagcc aacaatcgcg ctcgccgcca ccgaaccgcc caggtaacca aacagaccgg
     8701 taaagcccgc tgccgttcct gccgcttttt tcggtgccag ttccagcgca tgcagaccga
     8761 tcagcatcac agggccgtaa atcaggaagc cgataacaat catacaaatc atatcgacgg
     8821 ttgggttacc tgccgggttc atccagtaaa cgatagtcgc gatggtcacc agtgtcataa
     8881 agaaaacgcc ggttgcccca cggttgccac ggaagacttt atccgacatc cagccgcaca
     8941 gcagagtgcc cggaataccc gcatactcat agaagaagta ggcccaggag gatttatcca
     9001 gcgcgaaatg cttaacctct ttcagataag tcggtgacca gtcgaggatg ccgtaacgca
     9061 gcagataaac gaacacgttg gcgatggcga tgtaccacag cagtttgttc ggcagcacgt
     9121 actgcatgaa gatttgcttc gcagtcagct cctgttccgc tttttcgtta tagtcgtccg
     9181 gataatcatt tctgtactct tcgatcggcg gcaggccaca ggattgcggg gtatcgcgca
     9241 tcatcgcaaa ggcgaataat gccaccagaa tggcgcagaa agcgggcata tagagcgccg
     9301 cgtgccagtc attgaaccag gccatcccca gcaggaacag cagcggcgga ataccaccac
     9361 cgacgttgtg cgcacagttc cacactgaca caatgccgcc acgttctttc tgcgaccacc
     9421 agtgcaccat agtacgacca cacggcggcc accccatccc ctggaaccaa ccgcagagga
     9481 acaacagtac aaacatcacc gcaatgctcg acgtcgccca tggcacaaag cccataaaca
     9541 acatcactgc cgccgccaga atcaaacctg cgggcaggaa aacgcgcgga ttcgagcgat
     9601 ccgataccga acccatgatg aatttcgaaa atccataggc gatcgagatc ccagaaagcg
     9661 caaaacctaa atcaccgcgt gagaatccct gctccaccag gtaaggcata gcgagcgcaa
     9721 agttcttacg aaccaaatag taagcagcat aaccaaagaa tatccccagg aaaatttgcc
     9781 agcgcaatcg acgataagtc ggatcgatct ccgcggcagg taagcgcgct ttgtgtggcg
     9841 ctggtttaaa aatactcaac attgatagcc tccgtggccc gtgatcttat ttatgattaa
     9901 cagcctgatt cagtgggaga acctgccgtt tcttgagttg ccgcgatgtt aagaaaacat
     9961 tcataaatta aatgtgaatt accgcacaca ttattaaata cgatggtgaa aatgttcaga
    10021 atgacgcatg aaatcacgtt tcactttcga attatgagcg aatatgcgcg aaatcaaaca
    10081 attcatgttt ttactatggc taaatggtaa aaaacgaact tcagagggat aacaatgaaa
    10141 actcgcgact cgcaatcaag tgacgtgatt atcattggcg gcggcgcaac gggagccggg
    10201 attgcccgcg actgtgccct gcgcgggctg cgcgtgattt tggttgagcg ccacgacatc
    10261 gcaaccggtg ccaccgggcg taaccacggc ctgctgcaca gcggtgcgcg ctatgcggta
    10321 accgatgcgg aatcggcccg cgaatgcatt agtgaaaacc agatcctgaa acgcattgca
    10381 cgtcactgcg ttgaaccaac caacggcctg tttatcaccc tgccggaaga tgacctctcc
    10441 ttccaggcca cttttattcg cgcctgcgaa gaagcaggga tcagcgcaga agctatagac
    10501 ccgcagcaag cgcgcattat cgaacctgcc gttaacccgg cactgattgg cgcggtgaaa
    10561 gttccggatg gcaccgttga tccatttcgt ctgaccgcag caaacatgct ggatgccaaa
    10621 gaacacggtg ccgttatcct taccgctcat gaagtcacgg ggctgattcg tgaaggcgcg
    10681 acggtgtgcg gtgttcgtgt acgtaaccat ctcaccggcg aaactcaggc ccttcatgca
    10741 cctgtcgtgg ttaatgccgc tgggatctgg gggcaacaca ttgccgaata tgccgatctg
    10801 cgcattcgca tgttcccggc gaaaggatcg ctgctgatca tggatcaccg cattaaccag
    10861 catgtgatca accgctgccg taaaccttcc gacgccgata ttctggtgcc tggcgatacc
    10921 atttcgctga ttggcaccac ctctttacgt attgattaca acgagattga cgataatcga
    10981 gtgacggcag aagaggttga tattctgctg cgtgaagggg aaaaactggc ccccgtgatg
    11041 gcgaaaacgc gcattttgcg ggcctattct ggcgtgcgcc cgctggttgc cagcgatgac
    11101 gacccgagcg gacgtaacgt cagccgtggc atcgtgctgc tcgaccatgc tgaacgcgat
    11161 ggtctggacg gatttatcac catcaccggt ggcaaactga tgacctatcg gctgatggct
    11221 gaatgggcta ccgacgcggt atgccgcaaa ctgggcaaca cgcgcccctg tacgaccgcc
    11281 gatctggcac ttccgggttc acaggaaccc gctgaagtta ccttgcgtaa agtcatctcc
    11341 ctgcctgccc cgctgcgcgg ttctgcggtt tatcgtcatg gcgatcgcac gcctgcctgg
    11401 ctgagcgaag gccgtctgca ccgtagcctg gtatgtgagt gcgaagcggt aactgcgggt
    11461 gaagtgcagt acgcggtaga aaatttaaac gttaatagcc tgctggattt acgccgtcgt
    11521 acccgtgtgg ggatgggtac ctgccagggc gaactctgcg cctgccgcgc tgctggactg
    11581 ctgcaacgtt ttaacgtcac gacgtccgcg caatctatcg agcaactttc caccttcctt
    11641 aacgaacgct ggaaaggcgt gcaacccatc gcctggggag atgcactgcg cgaaagcgaa
    11701 tttacccgct gggtttatca gggactgtgt ggtctggaga aggagcagaa agatgcgctt
    11761 tgatactgtc attatgggcg gcggcctcgc cggattactc tgtggcctgc agctgcaaaa
    11821 acacggcctg cgctgtgcca ttgtcacgcg tggtcaaagc gcgctgcatt tctcatccgg
    11881 ttcgctggat ttgctgagcc atctgccaga tggtcaagct gtgacagaca ttcacagtgg
    11941 actggaatct ttgcgtcagc aggcacctgc acatccttac tctcttctcg ggccacaacg
    12001 cgtgctcgat ctcgcttgcc aggcgcaggc attaatcgct gaaagcggtg cgcaattgca
    12061 gggcagcgta gaactggctc accagcggat tacgccgctc ggcactctgc gctctacctg
    12121 gctaagttcg ccagaagtcc ccgtctggcc gctgcccgcg aagaaaatat gtgtagtggg
    12181 aattagcgga ctgatggatt ttcaggcgca ccttgcggca gcttcgttgc gtgaactcgg
    12241 ccttgccgtt gaaaccgcag aaatagagct gccggaactg gatgtcctgc gcaataacgc
    12301 caccgagttc cgcgcggtga atatcgcccg tttccttgat aatgaagaaa actggccgct
    12361 gttacttgat gcgcttattc ctgtcgccaa tacctgcgaa atgatcctga tgcccgcctg
    12421 cttcggcctg gctgatgaca aactgtggcg ttggttgaat gaaaaactac cttgttcact
    12481 gatgcttttg ccaacgctgc cgccttccgt gctgggcatt cgtctgcaaa accagttaca
    12541 gcgccagttt gtgcgtcagg gtggcgtgtg gatgccgggc gatgaagtga aaaaagtgac
    12601 ctgtaaaaat ggcgtagtga atgaaatctg gacccgcaat cacgccgata tcccgctgcg
    12661 tccacgtttc gcggttctcg ccagcggcag tttctttagt ggcggactgg tagcggaacg
    12721 taacggcatt cgagagccga ttctcggcct tgatgtgcta caaaccgcca cgcggggtga
    12781 atggtataag ggagattttt ttgcgccgca accgtggcag cagttcggtg taaccactga
    12841 tcagacgcta cgcccatcac aggcagggca aaccattgag aacctgtttg ccatcggttc
    12901 ggtgctgggc ggatttgatc ccatcgccca gggatgcggc ggcggtgttt gtgccgtcag
    12961 tgctttacat gccgctcaac agattgccca acgcgcagga ggccaacaat gaatgacacc
    13021 agcttcgaaa actgcattaa gtgcaccgtc tgcaccaccg cctgcccagt gagccgggtg
    13081 aatcccggtt atccaggacc aaaacaagcc gggccggatg gcgagcgtct gcgtttaaaa
    13141 gatggcgcac tgtatgacga ggcgctgaaa tattgtatca actgcaaacg ttgtgaagtc
    13201 gcctgcccgt ccgatgtgaa gattggcgat atcatccaac gcgcgcgggc gaaatatgac
    13261 accacgcgcc cgtcgctgcg taattttgtg ttgagtcata ccgacctgat gggtagcgtt
    13321 tccacgccgt tcgcaccaat cgtcaacacc gctacctcgc tgaaaccggt gcggcagctg
    13381 cttgatgcgg cgttaaaaat cgatcatcgc cgcacgctac cgaaatactc cttcggcacg
    13441 tttcgtcgct ggtatcgcag cgtggcggct cagcaagcgc agtataaaga ccaggtcgct
    13501 ttctttcacg gctgcttcgt taactacaac catccgcagt taggcaaaga tttaattaaa
    13561 gtgctcaacg caatgggtac cggtgtacaa ctgctcagca aagaaaaatg ctgcggcgta
    13621 ccgctgatcg ccaacggctt taccgataaa gcacgcaaac aggcaattac gaatgtagag
    13681 tcgatccgcg aagctgtggg agtaaaaggc attccggtga ttgccacctc ctcaacctgt
    13741 acatttgccc tgcgcgacga atacccggaa gtgctgaatg tcgacaacaa aggcttgcgc
    13801 gatcatatcg aactggcgac ccgctggctg tggcgcaagc tggacgaagg caaaacgtta
    13861 ccgctgaaac cgctgccgct gaaagtggtt tatcacactc cgtgccatat ggaaaaaatg
    13921 ggctggacgc tctacaccct ggagctgttg cgtaacatcc cggggcttga attaacggtg
    13981 ctggattccc agtgctgcgg tattgcgggt acttacggtt tcaaaaaaga gaactacccc
    14041 acctcacaag ccatcggcgc accactgttc cgccagatag aagaaagcgg cgcagatctg
    14101 gtggtcaccg actgcgaaac ctgtaaatgg cagattgaga tgtccacaag tcttcgctgc
    14161 gaacatccga ttacgctact ggcccaggcg ctggcttaaa ctcctttctg atgcccggta
    14221 agcatgtggt taccgggcat ttttgcgtac acgattccgt gcccaatgta tgcgttgcaa
    14281 cgcagtgaaa attcctctga aaacgtctcg caaaggctga aactggcaga tgtcaaaggc
    14341 ctgggataac cgtaatgtcg cgtcatcata aatatcaggt gacggacaac catgaccgaa
    14401 tcaacaacct cctccccgca tgatgcagta tttaaaacct ttatgttcac acccgaaacc
    14461 gcacgggatt ttctcgaaat acatttacca gaaccactgc gcaagctttg caacctgcaa
    14521 accttacgcc tggaacccac tagttttatt gaaaaaagtt tacgcgctta ctactcggat
    14581 gttttgtggt ccgtggaaac cagcgacggt gacggctata tctactgcgt gattgaacat
    14641 caaagctctg cagaaaagaa tatggctttt cggctaatgc gctatgccac tgccgccatg
    14701 cagcgtcacc tggacaaagg ctatgacaga gtcccgctgg tggtgccgtt gctgttttat
    14761 catggcgaaa cctcgcccta cccgtactca ctcaactggc tggatgagtt tgacgatccg
    14821 aaacttgccc ggcagttgta caccgaagct tttccgttgg tggatattac catcgtacct
    14881 gacgatgaga tcatgcaaca tcggcgtata gctctgctgg aactgattca aaagcatatt
    14941 cgcgaccgcg atttaatcgg tatggtcgac aggatcacca cgcttttggt tagaggcttc
    15001 actaatgaca gccagctaca aacactgttt aattatctgc tgcaatgcga cgatacctcc
    15061 cgtttcaccc gttttattca ggagattgcc gaacgttcac cactacaaaa ggagagatta
    15121 atgactattg ctgaacggct acggcaggaa ggacaccaaa ttggctggca ggaaggtaaa
    15181 attgaaggct ggcaagaagg taaattagaa ggtttgcagc aaggtaaatt ggagggtctg
    15241 cagaaaggta aattagaagg tttgcaggaa ggcatgcatg agcaagccat taaaattgcc
    15301 ttgcgcatgc tggagcaggg ctttgaacgt gagattgtgc tggcgacaac ccaactcact
    15361 gatgctgata ttccgaactg tcattaaaag gagagattga tgactattgc tgaacggcta
    15421 cggcaggaag gacaccaaat tggttggcag gaaggtaaat tagaaggttt gcaggaaggt
    15481 atgcatgaac aagccattaa aattgctttg cgcatgctgg aacagggctt tgatcgtgac
    15541 ctggtgctcg cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta
    15601 acacaggccc acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg
    15661 gcccattttt gcctgattta aacatcgcca gtcgttgatc cagagcatcg ctgtagagca
    15721 tcgtgtcaac gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat
    15781 caggagccac agccagaaag ccagccgctt tacccgcagc acggatccgc cgaatactgg
    15841 tttcaataat tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag
    15901 aaagatccgc aggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca
    15961 ggttatccag tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt
    16021 gcgccatgta attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac
    16081 cacgttcacc gtagggagga tagcgcgtgg cagacaccac ctgacgtgcc tgatcggcag
    16141 tatcgaccat cgggatcagt agagtttgcg cgccaatatc caggacttgt ttaatcagcg
    16201 acttactgcc atccaccgga cggatcacgg gttggctggc atagggcgct accgcctgta
    16261 gctggtgata aagatcctga atggtgtttg gcgcgtgctc cccatcaatc agcaaccagt
    16321 cataaccgga agtggcggca atttctgcca tataggcagt tgttgagctt aaccacagac
    16381 caatttgcac ttcgcccttg cgtaaacgtt ctttaaaggg attggttaat aatgcgttca
    16441 tcgtgttcct taacctattt aatgatgtgc cacgtcggtc tgcgcaacag tacgatttac
    16501 acgcagagtg aaaataatca gcgaaccgat gaccgccacc gctgccagcg tcagtaatcc
    16561 cgccgcatcg ctggaaaata acgtttctgc tttcacgcgc aggatcgggg caataaaccc
    16621 gcccactgca ccaaacaggt ttacaaaacc aatccccgca gccagcgccg taccggaaag
    16681 cagttgtgtc ggcatcgtcc agaacaccgg ctgcacggca ataaacccga tggctgcaac
    16741 acacagcgct acgatcgcca gtactggaga aagcagcccg gacagaccaa taccaatgcc
    16801 cgccgccagt aatgtcagcg ctgcgacatt acgccgttcg ccggttttat cggaatagcg
    16861 cggaataagc caggtcccaa acaaggccgc aatccacgga atggcggtga ccaccgacgc
    16921 tgtaaagccc acttttgttc ccagcaatgc cgcaatctgg gtcggcagga agaaaattaa
    16981 tccgtaaacc gccacctgaa tggtcaggta gataatcgcc agttgccaga ctcgcccatt
    17041 acgcagcgca tcgctcagcc gagaagtcac tttctgttgt tcttcacttg ccagttgatt
    17101 gataagcaac gttttttctt gtttactcag aaaacgtgcc tgctccggtg tgtcatcaag
    17161 ccaaaagaat gtgaataccc cagcgccgac tgccaacaat ccttcaatca caaacatcca
    17221 gaaccagccg ggatgcccca taaatccatg catctccagc agcgcgccgg aaagcggtga
    17281 tcccagtgtt aacgccagcg gtgcgcccat atagaacagc cccataatgc tggcgcgatt
    17341 acgctgcgga aaccattgcg aggtgagata aatcatacca gggaaaaatc cggcctccgc
    17401 agcaccaagc agagtgcgaa ctatcagaaa tttcgcttca gtatcggccc atgccatggc
    17461 tgccgaaaga aatccccaca gcagtgttgt cgtaccaatc caggttctgg cccccagttt
    17521 gcgcatcaaa agattcgccg gaacacccag aaacgcatat accacaaaga aaatgcctgc
    17581 tcccagcgca taagcttcat tactcaaccc ggtatcaatc tggtaggtct gtttggcaaa
    17641 accgatattc gaacggtcga gaaacgccag cacatacagc gccaacataa acggaattaa
    17701 acgcacacgg tttttcttca ccacggcgtc aagcaaagcg gtgctcataa aaagctcctt
    17761 agaatatgtg ggcgttaccg ttgtcacagc aacgcccgga gtgattagtg gctgtagggg
    17821 cgtttcaaat tgcagtcacg attgagttcg acgccaaaac cgggtttatc aagcactaat
    17881 ttatgaatac gaccattcac cggaaccggc tcattgagca gaatcgggtc aaactgcgga
    17941 cgcatcgttg aacaatccgg actggtcatc aggaattcgc tgaatggcgt attagtgaag
    18001 gtgatcaccg catggtggga gtaaacagac gaaccgtgcg gcaccaccag ttgccccctg
    18061 gatttggcga ttgcggcaat ttccaccagc gtggttaagc caccgcacca gccaacatcc
    18121 ggctgcataa tgtcgatacc ggtttctgaa agcgtacgaa aagattgcag tgtgccgtgg
    18181 tgctcaccgc tggtgaccat cattccgact ggcgcgttgc gtttcagttc gcgataacct
    18241 tcatactgct gtggcggcag gcactcttcg atccatttca ggttataggg cgcgcaagcg
    18301 tgggccagtt tggtggcata gttcacgtcc tgactcatcc agcagtcgag cattaaccag
    18361 aaatcctcac cgcatttttc acgcatatcc gcgaccatag cggcatcttt gcggatcccc
    18421 gcatcgccat catgtggccc ccagtgcgtc ggcattttgc caccgataaa gcccatctct
    18481 tttgccagat ccggacgcgc acctgtggcg tagaactgaa tctcatcacg aacagcgccg
    18541 cctaaaagtt tataaaccgg aagcccgacc actttgccga acagatccca cagagccaga
    18601 tcgacacagg aaatcgtatt catcaccagg ccaccagagc cggagtaata cagggtggca
    18661 ttgagcattt gatcgtggat cagtttgata tcactgacac atttaccctc aatgaaacgg
    18721 ttaaggtgtt tttcgacgat aaaacagccc atttcaccgg ctgtcgaaac agcgaatccg
    18781 gtctggccgt tttctgcttc gacttcaaca accaacgtgc caagaacgtt aatgccaaat
    18841 gactggcgtg actgctcgta atcgcggtat ttactcatcg gggtggcaat atgatcgtct
    18901 atccagtgat tcgccccctg gtcgtgataa tcaccgccgc cagcgccttt ttctgctgtc
    18961 gcaccgccag taaaccaggc gcgaacctgt ttaatttttg gtagggtcat gatgttctcc
    19021 attgttatga ggcttgtaag tcaaagggac ttttccatcc caacagacgt gaaatatccc
    19081 tggcgcaggc aatggccttg cccgccagat aatcacggta ttcttcattg atttgtaagc
    19141 gggtaccgac caccgagatc gcagcggtaa gctcgttatt ggcgttaaac accggcgcag
    19201 cgacacaacg gacatcggcg taatcttcgc cgttgtcata gctccagccc tgacggcgaa
    19261 tacgcgccag ttcttcgtga agttgctgtg gatgagtaat cgttgtgggt gtcgcctgct
    19321 cccagaccag cccttcgata atactttgct gtacagttgc aggttgccag gcaagcaggc
    19381 atttacctat tccggagcga taaagcgaaa ggctttttcc ttcatgtgaa cgcacgctga
    19441 tagtggctga tgactccact ttcaaaatgt aataagcgct gccgttatca ataatcccca
    19501 ggtgacataa cagcccggtg gtatccatca gttgcgtcaa acgcggtcga gccagttccc
    19561 ggagatccat cttgcttaac gcatgaccgg aaagctccac cagcttggtc cacaaacaaa
    19621 aattttcctg gtgatcaagg ctaagaaaac gctgacgcct gagttcatta agcagcaaat
    19681 aggccgtgct tttagggatc cccaatgtgt caatgatcgt cgcagcatta caggggccaa
    19741 tgcgggcaat caaattgaga atatcaatag cccgtgtcag ggcgggaact ttgcttgatt
    19801 ccaacatact ggactccagt cttagatact ggaatcagtc tcgttgctca gggggagtaa
    19861 aattgtgaag cgcatcaaat cccgctcctc gtccaacgga ctggagaaat tacgcacaaa
    19921 tttgtgaatc agcgcacgga ttttttctta ttgagtacaa caggttggac aaattttgcc
    19981 ggagggggaa tgtgccggtg cagtaaaccg gcacagagtg gcggaatcag acggataagg
    20041 tcatggactc aacaacctca atccagccat gctcactggc gatgtcctgg ccgtttaacc
    20101 aacggcgcag catattcagt gccatcattg cgcacacttc ctgacggata gccaggctat
    20161 agcgtgtagt gctgaaacgc acacgcagag cgaaagtgcc atctggcgtg gctagcgcaa
    20221 agttgagatg ctcgttctcg aaacccgaaa cagccagtgc cagcccggca aaatggttgg
    20281 cccgccgttc tgtaatccag tgcgcagttt gcgccagggt ttcctcctgt gaaggaacca
    20341 cttcacacgc cagcagtgga gcacctgcgc gagaaagttg caaagccaat aaaccaccgg
    20401 tgaattgctc gctcaacgtc aggctgaact ggcggttttg caattcgcga ctgatctgcg
    20461 cgggcagtcc ttcggtgcct tcgaaaatca cgctctgtcc ggcaacacgt ttaacgtcca
    20521 gccacagttt ttccatcgcc tgttgctcgc ttgccggtcc ggtgagtttc agttcgatga
    20581 tcggcattga ggagcgatag cccattgtta cccccggcgg cagttgtaga gtgtccaggc
    20641 tttgtgccag atcgctttcc gaacgaccaa aggtagttaa acgcagacaa accggcggct
    20701 gtggtaaaga aaaacgctcg cgcagacgcg gcaggatttc gtgctcgacc atcaccttaa
    20761 attctgacgg tacgccggga gtaaagaaca tcaggcaacg attaagctgc acggcaaaac
    20821 cacaggcggt gcctaccggg ttattgataa attcagcact ggcaggcagc tccgcttgtt
    20881 tacggttgct cggtgccatt actcgtccac gttcgtggaa atagcgttcc atctctttga
    20941 gccaggcttc atgcagcacc aggccttcac cttttgctgt cgcagcggcg agtgcgctta
    21001 aatcatcgct ggtcggtccc agcccgccgt taacgatcag cacatcggcg tgctgactac
    21061 gttcgcgaag aatggtgact aagtcatcaa ggttatcccc caccgtattg cggcgagata
    21121 atggcaaccc ctgatgaaag aaaaaatcgg ccagccaggc agcgttagtg tcaacgattt
    21181 gcccgtgtaa cacttcatcc ccggtggata gcatttccac ttttaacatg gttatctcct
    21241 gcttttgtta tcgaatcact ataacgcaaa gggagataag gcgtagtaag aaactgaatg
    21301 gcgcgacaga atgccgcgcc ggaagggatc agaaactggc gttaacccca acatacgggc
    21361 cgtcagccac ggcgttgtcg cggttaccgt ctttacccga cagattcagg tagcgataac
    21421 ccgcctcaat actgactgga cgcataatgg tgtaacgcgc gccagcattc gcttcctcat
    21481 aacttttaat accgctggag agtgaatccg gagagtagta atactcgcca aacaaacgga
    21541 agctgttgcc aattttccac tgcaaaccac ctcctaccgc tgcggcataa ccttcatcgc
    21601 cgtaattcgg gttggtgtaa acgccttttc cgccaacggt cgccattaac ggcccgagag
    21661 gcagattcaa ccccagcccc acgccagcga cgtcaccgtc gtcgtcgtta tgtgtccagt
    21721 taccgcttaa cgccaggccc gtcgattcag tcccaaaacc gacaccaata ttggtgtatt
    21781 ctttacccgc ctggccgctg atgctcattg cattgaccga tgcagaaacc aacagcattc
    21841 cggccagacc tgctagcgca atttttttca ttatcccctt tcccacaagc aattaatcaa
    21901 aatccccaaa gctgcgggat tgtactgcct ggcgcagagg attcaatgag cgaaaaaagg
    21961 gatctagtca cttattgtaa ctaaatactt cttaaataat taaacgccga aaaaataaac
    22021 acactttacc gatacacaca aaaataataa taagtgcagt tatcatcgca aaagagatta
    22081 ctgtcacttt cacatatttc tgagttaccg tattctcatg gtttcttttt atttataagg
    22141 aatacccctg tgcgacaacg gactattgta tgccctttga ttcaaaatga tggtgcttat
    22201 ttgctgtgta aaatggccga cgatcgcggc gttttccccg gtcaatgggc gctttcgggt
    22261 ggcggcgtgg agcccggcga acgaattgaa gaggcactac gccgcgaaat tcgcgaagaa
    22321 ttgggagaac agctgctttt gacagaaatc acgccgtgga ccttcagcga tgatattcgc
    22381 accaagacgt atgcagatgg tcgcaaggaa gagatttata tgatttacct gatttttgac
    22441 tgcgtttctg ccaaccgaga agtgaaaata aacgaagagt ttcaggacta cgcgtgggta
    22501 aaacctgaag atctggtgca ttatgatttg aatgttgcca cccgaaaaac gttacgtttg
    22561 aaaggtcttc tttaacaatg atgacagcca tcactggctg tcagattgaa aatttagtgg
    22621 ttaacgaatt ccccatccag ataaactttg cctttttcca catgcatgac taaaccatcc
    22681 agataatcag gtttaaatgt cgcatcacgc ttatctttag caatatatgt caggcaatga
    22741 ttatgggtaa aaataacgat atttttatca ggtgcttttc tttgtaagtc tttaattgca
    22801 ctataaatct cattaccgca ttgaagaagt cgtttatcta ccgtgagctt tttacccgct
    22861 gaaaaccagg tcgcggactg aatggtccgg acggtattac tggaataaag atcgaaatca
    22921 gggatatcag cactaaaagc gttgcccagt tcacgggcat cctgggtacc tttgaccgta
    22981 atacctgttt tatctgacaa gcattgattg gttgaacgat cgcaacgttc agcatggcga
    23041 aacaaaacga caaccgggtg ctgctgtgcc agtctggcca gtgttttatt gtcgatacgt
    23101 ggcaaaccat tgctactcca ggcggcatga gtacccagtc cggcaattgc agcgagcgcc
    23161 agtaaaatga tgaaatattt ttttgacttc aacgaagagc ggcaaaaagc taacacgtaa
    23221 actccaccta tagacaagcg caaccagaca attaccgtga aattgagcga catttctggc
    23281 gataattagc agttggtgta atattaaaaa tcctgcgatg ccggcaaaat gcctcaaaat
    23341 tttgccaaat gcaaagccta aataagaaaa aatataaaaa tttcaatatt tacgtctaat
    23401 attagtttct taaggttaag ttaatattct atccttaaaa tttcgctcca aatggcaaaa
    23461 tatacacaac actctttata gcaaatataa gtggacaggt attcaatggc ggaaggaaaa
    23521 gcaatgtcag aatttttgcc tttttcgcga ccagcaatgg gcgtggagga actcgctgca
    23581 gttaaagagg ttctcgaatc cggttggatc acaaccggtc cgaagaatca cgcgcttgaa
    23641 caagcctttt gccagttgac aggaaatcag catgccatcg cggtcagttc agccaccgcc
    23701 ggaatgcata tcacgctaat ggcgttggaa attggcaagg gcgatgaagt gattacgcct
    23761 tccctgacct gggtttcaac cctcaatatg atttccttgc agggtgcaac gccggtaatg
    23821 gtggatgtcg atcgcgatac gctgatggtc acgcctgaag cgatcgaatc agccattacg
    23881 ccacgcacta aagccatcat tccggtgcat tatgccggtg cgccagcaga tattgacgcc
    23941 attcgcgcca ttggcgaacg ttacggcatc gcagttatcg aagatgctgc ccatgccgtc
    24001 ggtacgtatt acaaagggcg acatattggc gcaaaaggta ccgctatttt ttcatttcat
    24061 gccattaaaa atattacctg tgcggaagga ggcctgattg ttactgataa tgaaaatatt
    24121 gcccgccagc tacggatgct gaaatttcac ggcctgggtg tcgatgccta tgacagacaa
    24181 acctggggcc gtgcaccgca ggctgaagtc ttaacaccgg gctataagta caatctgacc
    24241 gatattaacg ccgcgattgc cctgacacag ttagccaaat tagagcacct caacacccgt
    24301 cggcgcgaaa ttgcccagca atatcagcaa gcactggcag ctctcccctt tcagccatta
    24361 agccttcccg cctggccgca cgttcacgcc tggcatctgt ttattattcg tgtcgatgaa
    24421 caacgttgtg gtatcagtcg cgatgcgttg atggaagcgt taaaagaaag aggtattggt
    24481 accgggttac atttccgcgc cgctcacaca caaaaatatt atcgcgagcg ttttcccacg
    24541 ctgtcgttac cgaataccga atggaatagc gaacgcatct gttcgttgcc gctgttcccg
    24601 gatatgacta ccgccgatgc cgaccgtgtc atcacagccc ttcagcaact cgcaggacaa
    24661 taagccatgt ttgaaatcca ccctgttaag aaagtctcgg tggttattcc cgtttataac
    24721 gagcaggaaa gcttaccgga attaatcagg cgcaccacca cagcctgtga atcgttgggg
    24781 aaagagtatg agatcctgct gattgatgac ggcagtagcg ataattccgc gcatatgctg
    24841 gtcgaagcct cacaagcgga gaacagccat attgtgtcta ttttgcttaa ccgcaattac
    24901 gggcaacatt cagcgattat ggcgggattc agtcacgtta ctggcgactt aattattacc
    24961 cttgatgccg atctccagaa tccgccagag gagatccccc gcctggtggc aaaagccgat
    25021 gaaggttatg acgtggtagg gactgtacgc cagaaccgcc aggacagctg gtttcgtaaa
    25081 acagcttcga agatgattaa ccggcttatt cagcgcacca ccggcaaagc gatgggtgac
    25141 tacggttgta tgctgcgcgc ctatcgccgt catattgtcg atgcgatgtt gcactgccat
    25201 gaacgcagca cctttatccc gattctggcg aatatcttcg cccgccgtgc cattgaaatt
    25261 ccagtacatc atgccgagcg tgagtttggt gaatccaaat acagttttat gcgcctgatt
    25321 aatttgatgt acgacctggt gacctgcctt accacaacgc cgctacgtat gctgagtctg
    25381 ctcggcagca ttattgcgat tggaggtttt agcattgcgg tgctgctggt gattttacgc
    25441 ctgaccttcg gaccacaatg ggcggcagaa ggcgtcttta tgctatttgc cgtgctgttt
    25501 acttttattg gcgctcagtt tatcggcatg ggattactcg gtgaatatat cggcaggatc
    25561 tacaccgatg tccgcgcccg cccccgctat tttgttcagc aagttatccg tccatccagc
    25621 aaggaaaatg aataatgaaa accgtcgttt ttgcctacca cgatatggga tgcctcggta
    25681 ttgaagccct gctgtctgcc ggttacgaaa ttagcgccat ttttacccat actgataatc
    25741 ccggtgaaaa agccttttat ggttcggtgg ctcgtctggc ggcggaaaga ggcattccgg
    25801 tttatgcgcc ggataacgtt aatcatccgc tgtgggtgga acgcattgcc caactctcac
    25861 cagaggtgat tttctctttt tattatcgcc atcttatttg cgacgaaatt ttgcagctcg
    25921 ctcccgcagg tgcatttaat ctgcatggtt cgctgttacc aaaatatcgt ggtcgcgcgc
    25981 cgctgaactg ggtgctggtc aacggtgaaa cggaaactgg cgttacattg caccgaatgg
    26041 tgaaacgtgc cgatgccggg gccattgtgg cgcaactgcg cattgccatt gcgccagacg
    26101 atatagctat tacgctgcat cataaattgt gccatgccgc gcgccagcta ctggaacaga
    26161 cattacccgc cattaaacac ggtaatattc tggaaatcgc ccagcgcgaa aacgaagcca
    26221 cctgttttgg tcgcagaacg ccggatgaca gtttcctcga atggcataag ccggcatccg
    26281 tactgcacaa catggtacgt gccgttgccg atccgtggcc gggtgccttc ggctatgctg
    26341 gcaatcagaa attcaccgtc tggtcgtcgc gtgttcatcc tcatgccagc aaagcacagc
    26401 cggggagcgt gatttctgtt gcgccactgc tgattgcctg tggcgatggc gcgctggaaa
    26461 tcgtcaccgg acaggcgagc gacggcatta ctatgcaggg ctcgcaatta gcgcagacgc
    26521 tgggcctggt gcaaggttca cgcttgaata gccagcctgc ctgcaccgcc cgacgccgta
    26581 cccgggtact catcctcggg gtgaatggct ttattggcaa ccatctgaca gaacgcctgc
    26641 tgcgcgaaga tcattatgaa gtttacggtc tggatattgg cagcgatgcg ataagccgtt
    26701 ttctgaatca tccgcatttt cactttgtcg aaggcgatat cagtattcat tccgaatgga
    26761 ttgagtatca cgtcaaaaaa tgtgatgtcg tcttgccgct ggtggcgata gccacgccga
    26821 ttgaatatac ccgcaacccg ctgcgcgtat ttgaactcga ttttgaagag aatctgcgca
    26881 ttatccgcta ctgcgtgaag taccgtaagc gaatcatctt cccgtcaact tcagaagttt
    26941 atggcatgtg tagcgataaa tacttcgatg aggaccattc taatttaatc gtcggcccgg
    27001 tgaataaacc acgctggatt tattcggtat caaaacaatt acttgatcgg gttatctggg
    27061 cctatggcga aaaagagggt ttacagttca ccctcttccg cccgtttaac tggatgggac
    27121 cacgactgga taaccttaat gcggcacgaa tcggcagctc ccgcgcgatt acgcaactca
    27181 ttctcaatct ggtagaaggt tcaccgatta agctgattga tggcggaaaa caaaaacgct
    27241 gctttactga tattcgcgat ggtatcgagg cgttataccg cattatcgaa aatgcgggaa
    27301 atcgctgcga cggcgaaatt atcaacattg gcaatcctga gaacgaagcg agtattgagg
    27361 aactgggcga gatgctgctg gcgagcttcg aaaaacatcc gctgcgccat catttcccac
    27421 cgtttgcggg ctttcgcgtt gtcgaaagta gcagctacta cggcaaagga tatcaggacg
    27481 tagagcatcg taaaccgagc atccgcaatg cccaccgctg cctggactgg gagccgaaaa
    27541 ttgatatgca ggaaaccatc gacgaaacgc tggatttctt cctgcgcacc gttgatctta
    27601 cggataaacc atcatgacca aagtaggctt acgcattgat gtcgatacct ttcgtggcac
    27661 ccgtgaaggc gtgccgcgtc tgctggaaat cttgagtaag cataatattc aggccagcat
    27721 ttttttcagc gtcggcccgg acaatatggg ccgccatctc tggcgactgg tgaagccaca
    27781 gtttttgtgg aagatgctgc gctcaaacgc ggcatcgctt tatggctggg atattttact
    27841 ggcaggtacg gcctggccag gtaaagagat tggtcatgcc aatgccgata tcattcgtga
    27901 agcggctaaa catcacgaag tcggcctgca cgcctgggat caccatgcct ggcaagcccg
    27961 tagcggtaac tgggatcggc aaacaatgat cgacgatatt gcacgcggcc ttcgcactct
    28021 ggaagagatt atcggtcaac cggtaacctg ttctgccgct gcgggctggc gtgccgacca
    28081 gcaggtgatc gaagcaaaag aagcgttcca tttgcgctac aacagcgatt gtcgtggggc
    28141 catgccgttc cgtccactgc tcgaatcagg aaaccctggc actgcgcaaa ttccggtgac
    28201 cttacccacc tgggatgaag tgattggtcg ggatgtgaaa gcagaagatt ttaacggttg
    28261 gttactcaac cgcatcctgc gagataaagg cacgccggtt tataccattc atgcagaagt
    28321 tgaaggctgc gcttatcagc ataattttgt ggatctcctc aaacgcgcag ctcaggaagg
    28381 cgtgacattt tgccctttaa gcgaactgtt atcagagacg ttgccgctcg gacaagttgt
    28441 tcgcggaaat attgccggac gtgaaggatg gctgggttgc caacaaattg cgggtagtcg
    28501 ctgatgaaat cggtacgtta ccttatcggc ctcttcgcgt ttattgcctg ctattacctg
    28561 ttaccgatca gcacgcgtct gctatggcaa cccgatgaaa cgcgttatgc ggaaatcagt
    28621 cgggaaatgc tggcatccgg cgactggatt gtgccccatc tgttagggct acgttatttt
    28681 gaaaaaccca ttgccggata ctggattaac agcattgggc aatggctatt tggcgcgaat
    28741 aactttggtg tgcgggcagg cgttatcttt gcgaccctgt taactgccgc gctggtgacc
    28801 tggtttactc tgcgcttatg gcgcgataaa cgtctggctc tactcgccac agtaatttat
    28861 ctctcattgt ttattgtcta tgccatcggc acttatgccg tgctcgatcc gtttattgca
    28921 ttctggctgg tggcgggaat gtgcagcttc tggctggcaa tgcaggcaca gacgtggaaa
    28981 ggcaaaagcg caggattttt actgctggga atcacctgcg gcatgggggt gatgaccaaa
    29041 ggttttctcg cccttgccgt gccggtatta agcgtgctgc catgggtagc gacgcaaaaa
    29101 cgctggaaag atctctttat ttacggctgg ctggcggtca tcagttgcgt actgacggtt
    29161 ctcccctggg gactgacgat agcgcagcgg gagcctgact tctggcacta ttttttctgg
    29221 gttgagcata ttcaacgctt tgcactggat gatgcccaac atagagctcc gttctggtac
    29281 tacgtgccgg tcatcattgc cggtagtctg ccgtggctgg gattactccc cggtgcacta
    29341 tacacaggct ggaaaaaccg caagcattcc gcaaccgtct atttgttgag ctggacgata
    29401 atgccgctgc tgtttttctc cgtcgctaaa ggcaaattgc ccacctatat tctttcttgc
    29461 tttgcacctc tggcaatgct gttggcgcat tacgctttgc tggcagcaaa aaataatcct
    29521 ctggcgctgc ggattaatgg ctggattaac atcgcttttg gcgtcactgg cattattgcc
    29581 acatttgtgg tctccccctg gggaccaatg aacacgccgg tgtggcaaac cttcgagagc
    29641 tataaagtct tttgtgcctg gtcgattttt tcgctatggg catttttcgg ctggtacacc
    29701 ttaacaaacg tcgaaaagac ctggtctttt gccgcgcttt gcccgctggg gctggcgttg
    29761 ctggtaggat tttcaattcc tgacagagtc atggaaggaa aacatccgca attttttgtc
    29821 gagatgacac aagaatcact tcagccaagc cgctatattc ttactgatag cgtcggtgtt
    29881 gccgcaggtc tggcatggag cctgcaacgc gatgacatca tcatgtatcg ccagacaggt
    29941 gagttgaaat acggccttaa ttatccggat gcgaaaggga gatttgtcag cggtgatgag
    30001 ttcgcaaact ggcttaatca acatcgtcag gaggggatta ttacactcgt gctttcggtt
    30061 gaccgcgatg aagatatcaa cagtctcgcc attccgcccg cagatgccat cgatcgtcag
    30121 gagcgtctgg tgctgattca gtatcgtccc aaatgatctg gctaacatta gtctttgcca
    30181 gcttgcttag cgttgccggg cagttgtgtc agaaacaggc aacctgcttt gtggcgataa
    30241 acaaacggcg caaacatatc gtgctgtggc tgggactggc gctggcttgt cttggtcttg
    30301 ccatggtgct ctggctgctg gtcttgcaga acgtaccggt aggcattgcc tacccgatgt
    30361 taagtctgaa ttttgtctgg gtgacgctgg ctgcagtaaa actgtggcac gaaccggtat
    30421 cgccgcgtca ctggtgtggg gtggcgttca ttattggtgg cattgtgatc ctcgggagta
    30481 cggtgtaatg ggcctgatgt ggggattatt cagcgtcatt attgcctcag ttgcgcaatt
    30541 aagcctgggt tttgcggcga gtcatctgcc gccgatgacg cacttatggg attttatcgc
    30601 ggctctgtta gcttttgggc tggatgcccg gatactgtta ctcgggctgc tgggttatct
    30661 gctttccgtt ttttgttggt ataaaacgct acataaactt gcgctaagca aagcctatgc
    30721 cttgctcagt atgagttatg tgctggtatg gattgcttca atggtacttc ccggctggga
    30781 agggactttt tcgctgaaag cactactggg agtagcctgt attatgagcg ggttgatgct
    30841 gattttcctg cccacgacaa aacaacgtta ctgagttttc cctgccactt tacactgccg
    30901 ttcccactca tcggggctat aactgcttgc cgagaggact ttcaccgtat gcagcttttc
    30961 acgattaata caataaagcg cattctgcaa aggcgagagt aaatccccca ctttcacggc
    31021 gaagtcactc ttcacttcgg cgatcatttt tatcgcgccg ccagcatcgc agagcataag
    31081 tacatgtctg ttatcttgtt tattgcagca cgattttttg accagccatt ccattgcatt
    31141 atcctgtttg ctaagagttt tcacatcaat ttacagatta acagataaac tctttatcaa
    31201 cggcaatgga atggctgaaa tgagaagtga aacggagggt taattattgc tgacgttgca
    31261 cccactcttt tagcgcctga cgtgaaattt taataccgcc gtttttcagt tcaggcggca
    31321 acgttaacca acgtaccggt tgctgaaaac gggccagctt atctttcacc cattcactaa
    31381 gatcaacgct ctcgtggtca tactccatca ccgccaccgg tcgatgacca aactccttgt
    31441 cggcaacggg gacgataaac acctgcaaaa ccgcaggatg cgcagcaatt acgcgctcga
    31501 cttcttccgg ctgaataccc tctccgccac tgaagaatag attgtctaaa cgtccgacaa
    31561 tggtcagctt gccattatgc atctcaccgc gatcgcgcgt agcgtaccag ccttcgtcat
    31621 taaccagtga aaccagttgc ccgttacgcc agtaaccttc tgccatactg gcagcccgca
    31681 gccacacttc attattaacg attttcactt cccgacccgg cagcggcgaa ccaacgtctg
    31741 ccaggccgtc ggcttctttc gcacacaccg tggaggcaaa ctcggtcaga ccatagccgc
    31801 aaaagcaacg aatcccctgc tcgcgcgcct gttccgtcaa ctcgaccggg atagccgcgc
    31861 cgccaagtaa caccgctttc agggaaacgg aactgcggtt aaccagcaaa cgccagagtt
    31921 gtgttggcac cagtgaagcg tgcgtacagc ctgccagcat ttgctccaat ggttgtttat
    31981 cacgtaccgt catccgcgca ctagcgtata accagcgcca cataattccc tgaccggaga
    32041 cgtgaaacag cggtaaagag agcaaccagt catcgtgatc gccaaacgga atcaacgata
    32101 acaccccttc ggcactggca agatgggcct gataagtatg gacagcggct ttcggcaaac
    32161 cggtagaacc cgaggtcaac gtcattgagc acagacgcgt cggctgccac gtagcggcat
    32221 gtgcgccttc aacccgctga atgtgcagcg acgttaacgc cggaaacgtg ttttccccat
    32281 ccggcaccag cgcaaattgc agcgtcagat tgggcagcaa ttcttcaagc aacggttgcg
    32341 gcagctgagg gttcacgggc aacacccgcg ccccgcattg cagtaacgcc agccaggcga
    32401 gcagcgtttg cggcgtattc cacgcccgca acatcacgcc gctgccctca accacaccct
    32461 gcaccgcaaa tccggaggct aattcatcga cgcgagcaca aagctcgcgc cagttgagtt
    32521 gctcgtcatt aagacgtaag gcgatggctt ctccccgcac ttgccgccag tgacgccacg
    32581 gccagtcaga gaagatcata acaaccgctc cagtgcatca acttccacga caggcagcgg
    32641 gctacccggc cagcgacgaa cctgctgcgc ctgcatcaga tccagcgtgt ccagccccgg
    32701 aatggtgtct ggcgttaacc aggcggcaat ccgcgccagt tgcgttaagc ctaagctcga
    32761 ctcaatagaa gaactgatca ccgccgtcag ccccaacgcg tgcgccgcct gcacctgctc
    32821 gcgtacttta tccaggctgc ccgtgagcgt gggcttgata actaccgcgc gcacaccttc
    32881 ttccgccaca aaggcaaaat ctggctcacg caggctttca tcccaggcaa tggcaatccc
    32941 ggtttcacgg gcaaacgcac gcgaatcatc gcgggttttg cacggctctt cgagaaacgc
    33001 gatgcggtgg cgataatccg ggttaacata tttggcaaac tgctgacctt tcagcggtgt
    33061 ccaggcgcga tttgcgtcaa gacgcaaatg cagatccgga attgcctcca gcaacagatt
    33121 caccaccatg ccgtcgcgca ccgcttcgta caatcccact ttgaccttcg ccactttctc
    33181 gcctggcata tctgcaagtt tgaggatcag atcgtccgga tcgccattac acagtggtgc
    33241 cgtacggtaa ttggccgcct gcgggagcgt atctgccagc tccgccaatg cacaacttac
    33301 gccaaagcca acggaaggca tctgcggtaa ttcgcaatcg cctgccagcc agttatttac
    33361 ccaggcaagc agcacgcttt gcgcctcttc cctggtttcc tggctaaagc ccggcagtgg
    33421 ggagatctcc ccccaccctt cgcgctcgcc ctcacgcagg caaacgtaca gcccgtcgcg
    33481 ggtttttaac cgcctgtcgc gcagaaccac ccccgcgtcc atggggatct gccagcggta
    33541 tacctgcgcg ctacgcatta cggattccgt ttgaatttgc tgaagtcagg ctgacgtttc
    33601 tggttgaagg cgttgcgacc ttcctgacct tcttccgtca tgtagaacag catggtggcg
    33661 ttgcccgcca gctcctgcag acctgcctga ccgtcacagt cggcgttcag tgctgctttc
    33721 aggcagcgca gcgccatcgg gctgttttgc agcatttcgc ggcaccaacg aacggtttct
    33781 ttttccagat ccgccagcgg taccacggtg ttcacaaggc ccatatccag cgcctgtttt
    33841 gcgtcgtact gacggcacag gaaccagatt tcacgcgctt ttttctgccc gacgatacga
    33901 gccatgtagg aagcgcccca gccgccgtcg aaggaaccga ctttcgggcc agtctgaccg
    33961 aagatggcat tatctgccgc gatagtcagg tcgcacatca tgtgcagaac gtgaccaccg
    34021 ccgatggagt agccagccac catcgcgaca accggtttcg gacaggtacg gatctgacgc
    34081 tggaagtcca gcacgttcag gtgatgtacg ccggaatcat ctttatagcc gccgtaatca
    34141 ccacgcactt tctggtcacc accggagcag aacgctttat cgcctgcgcc agtcagaatg
    34201 atcacgccga cgttatcgtc ataacgcgca tctgccagtg cctggatcat ctcttttacc
    34261 gtcagaggac ggaaggcatt gcgcacctgc ggacgattaa tggtgatttt tgcgataccg
    34321 tcggtggatt tttcataacg aatgtcctcg aaaccttcgg agcagtcgtg ccattcaacc
    34381 ggtgcgtaaa gcattgcttc atcaggataa atcatattgt gtcctttagt cagaaacgca
    34441 agatctgcgc cagacttgcg ataacgccag cgggattttc ccgatgcgcg ttatgtccgg
    34501 cgcgaggaat gacatggcag tcggcagcca gttccgccgc cagggcgagg aatttgctgt
    34561 cacgttcacc acataaataa taaaacgcaa atgtgcgggc gctaaggtta gcacgtaaat
    34621 caggctggac ggcgagagaa gtcgcctcca gcatggcggc aagggttgcg ccattattgt
    34681 tgctgcgcag cgccaccagc tcccggcgtt gatcgtcatt gagcgaggca aaaacaggct
    34741 gttgatacca gtcggcaaat accgccgtta acggttctgt gcgaaaacgc tgcgcccatt
    34801 ggcgatcgga acgctgacgt tccgcacgtt gttcagcatt ttgcagcccc ggatgcccgc
    34861 cttcgacaat aaccccacaa agccccgcca gcccctggca agcggccatc atcgccaccc
    34921 gtccgccaag cgagtacccc accagccaga atctaaggat gttgtaacta accagggttt
    34981 tacgcagtaa gtcggtgaca tcatcaaatc catcgacgct aatcgccgcc gaaccaccgt
    35041 gacctgggag atcaacatac aaccgtgagt agtcggcaaa cgcctcgccg acttcctgcc
    35101 attcgaggca atcgccggaa aaaccgtgca aaaacaccag ccagggtaaa cctggtttgc
    35161 cgtgttttgc ctgcgcgtgc aggatcataa atggcttacc tgcgccagca gttgctggag
    35221 cgtttgcgcg ccgtcggtgt cgttaaccac catttcaatt accgtggtgg tcggagtacg
    35281 ccaggcgtcg gcaagtaccg tttcaagttc ctgccagttt tgcggacgat gatatttcag
    35341 ctcaaacatc gcggcagcgt gctcaaaatg gacgttttgc ggcatcagat aaaaacgctc
    35401 gcgctcgctt ttcggcgtcg gcagcagcga gaaaatttgc ccgccgttgt tgttcaccac
    35461 aattaatacc agcggcgcgg aaacctggcg caataacgcc agcgcattca ggtcgtaaag
    35521 tgcggagaga tcgcccacaa tcgccagcgt cggtttgccg cttgcccgct gaacgccggc
    35581 ggcagtcgag agcagcccgt cgataccgct ggccccacgg ttgctgtaca ccgggtaacc
    35641 tgccggaagt tgcgaaagcg catcaatcag acgtaccacc aggctgttac cgacaaacaa
    35701 ttgcccctgt tcaggcagat agtcgctgat gcgatgcgcc agttgcgctt cgccaaacgc
    35761 atcgcgacgg gcaataaccg cctgcattgc ctgttccgcc aggcgcggga tttcaacgca
    35821 ccagggctgg cgtttttctg ccggatgcag ctccagccag tcggcaatat tggcaattaa
    35881 gcggcgtcca cgatggtgtg ccggatcaag tcgcccttca atgtcatcca caatccagta
    35941 ctcttcgggt tcacagcttg cctgccattg caggagccgc ttgccagtca ggctgcttcc
    36001 cagttgcacc acaatttgcg cctgttgcag ttcgctggtc gctttggcat tgcctaacca
    36061 gagatcggca cacggcagcg gctgtccggt ttgcgacagc acgtcgccaa tcagcggcca
    36121 gccaagagtt tgcgcccaca gtgcaacttt tttgccctct tccgcactca tgcgcccggc
    36181 aaccaccacg ccgcgttttt gccgccagaa gaaccagtcg cgctgttttt cactttccag
    36241 acgaggcgct tcacgcagcc acggcttatc gtcctgccac cagtcgccca gacgctgttg
    36301 ccagctaagc ccggtatcgt ccatttcgcc atacagcggt tcagcaaacg ggcagttgat
    36361 atggactcca cccgcatgaa gcgtaccgag agcgtggtcg atggtagaaa ccagccaacg
    36421 tgcggggata tcctgggtcg ggcgcggcaa tgaaatactg tgcgtggggt gagaggcgaa
    36481 cattcccggc tggcgaattg cctgattcgc gccgcagtca attagctccg gcgggcgatc
    36541 ggcggttaag agaatcagtt tttctccggt taacccggct tcaatcagtg ccggatagag
    36601 atttgccacc gccgtaccgg aggtgacaat caccgccacc ggctgcttgc tgactttcgc
    36661 cagccccagc gccagatgcc ccagtccacg ctcatcgaaa tgggtgtggt gaatgaatgc
    36721 ggaattctcc gccgccgcta acgttaacgg tgtagaacgc gagccagggg cgatacagat
    36781 gtgtcttacg ccgtgacgcg ttaatgcttc cagaatgacc gccgcccagc gtcggttaaa
    36841 tgcgcttact gacatgagtt tgtccggtat caatattgcg gctaagtata aggagctaca
    36901 aaaatagatt attgatatga atcggtaatg atgcgactca ttactattcc atttgtaata
    36961 aagtacgcag ccctgccgct ttgttgtcga tttcctgcca ctcttgctcg gggtcggaac
    37021 cacggacaat gcccgcgcca gcatatagcc gcacgacatt gccgctgatt tttgctgagc
    37081 gcagggaaac gcagaattcg ctttgttgta atgagagata gcccgcagaa ccagcgtacc
    37141 attctcgggt gaacggttcg tgacgggcga taaactgtcg cgccagatcg cgcggtaaac
    37201 cagcaactgc tgccgttggc tgcaactgat gtaaacagat cacatcatcc gctttgttga
    37261 gtgaagtcca gatacagcgg cgaagatgct gcactttacg cagacgcagt acctgcggcg
    37321 gtaaaacatc cagcgtctgg gtatcggcct gtaatcgttg gcagatatct tccaccacta
    37381 acatattctc acgctgattt ttatcatccg ccatcagcca ctcacctaac tgctgcgcct
    37441 gcttatcatc aggatgattt gctactgttc ccgccagcgc ttcagtacgc agcgctttgt
    37501 cacgccgccg ccataaccgt tccggtgaag agccaagaaa agcagtttcg ccatcaaagg
    37561 ccatgtaaaa atggtagcaa ttgagattca gtcgacgact ggcagccatc atcgccgccg
    37621 cattgaccgg acttgcgaaa tgcaggtcag ttgcccgagc aagcaccact ttgtcgagct
    37681 caccttcggc gatggttttc gttgccagtt cgattaattg cgtccagccc gttttgtccg
    37741 gccagtgttg ttctcgcgta gtggttaaat gtaacccagg caagggcttg atactcacca
    37801 gtgtggcgat aaattctctt gccttaatcg catcgtgctg aagggagctt tcgctgaata
    37861 gcgtcagccg cagcgtggct ttaccgccac agcgtcgcca ttccaggcgt ggtaaaagta
    37921 aattgccctg cgacgggtca aacgcattca gcccccaaat gcgtaagtcg gcgtgttccg
    37981 ggtgctggcg aagaaaacgt tgtgcctggt ccaacgacgt aaaacgggta atcgcgccca
    38041 ggacggcagc ttcttcatca ccattacgtt gttgccagta gaattgcggg tacgtttgct
    38101 gactggccag ccagctcaag gcatcaaaag cgtctttgag agggaaagga atatcgataa
    38161 cccgaatgcc gggtgtcgcc ggaatctctt gcgacaaatg gcgcagtaaa ttttccagcg
    38221 ccgtagtaag tgattgcacg cggacctctc cctgttaaaa gcctcacatt atacggggta
    38281 ctacaaaaaa aatgcagtac cccggtgtag ggaggtttta acggcgtgcc agcaacagtc
    38341 ctagtaccag cccaacggct gcgcccacac caattccttg ccagggtttt tcgtggacgt
    38401 agtcatcagc acgataaaca gcctgcttcg cccgatagta ataactgtct gaagcctggc
    38461 taacccgttt tttcacatca tccagcgctt tttctgcacg cgctttcagc tcaacatatt
    38521 tctgatcggc gggatcgcca gaggagcgga gcacctcttc cagtgtttca ctaagcagcg
    38581 tcaggtcgtc atcgatacgt gtatcaccaa actgattaga cattctcgtt ctccatttgc
    38641 gtaaaacctg tcactaacta tagacaaggg ttattgatta cgcctgaatt acctcgcgtg
    38701 ccatcccaat gtgtgggata ccatcttctt catagatctc cgtcacaggg ataaaaccga
    38761 aactctggta gaagttttgc agatgcgcct gcgccccgag gtagacaggc ttatcaggcc
    38821 agtgatgcgt gcaagtgtcg agggttttac tcatcagttg ctgccccact ttttcgccac
    38881 gcaacgcttc actgacaatc acccgaccaa taacgaccgg ctcaagatca tcatcacttt
    38941 tcagaatcct cgcatacgcc accagttcat catttttcca accgagaata tggcgattat
    39001 cccccgtcaa atcatcacca tcgatatcct gataagggca attctgctcg acaacaaaca
    39061 ccgcgcaacg cagttgtaat aaggcataaa gctgggaaac cgataattca gagtgatgca
    39121 gatcttgcca ttcaatcata atgctctcct tttagatgta tgccttatac tagccggaat
    39181 attttttgaa acgtgatgaa ctcatggaat taattttttt aggtacttcg gccggtgtgc
    39241 caacccgcac gcgcaatgtc acggcaatat tgcttaacct gcaacatccg acccagagcg
    39301 gactttggtt gtttgactgc ggtgaaggca cccagcatca gctactgcat accgccttta
    39361 accctggaaa actggacaag atttttatca gtcaccttca tggcgatcat ctttttggtt
    39421 tacccggctt gctgtgcagt cgttctatgt caggcattat ccaaccctta acgatttatg
    39481 gtccgcaagg tatccgtgaa tttgtggaga ccgcgctgcg gattagcggc tcatggaccg
    39541 attatccgct ggaaattgtc gaaattggcg ctggcgaaat tctcgatgat ggcctgcgca
    39601 aagtgaccgc ttatccaatg gaacacccac tggaatgtta tggctatcgt attgaagaac
    39661 atgataaacc gggtgcatta aatgcccagg cattaaaagc tgctggcgtg ccgcctggcc
    39721 cactgtttca ggaattaaaa gcgggcaaaa caatcatgct ggaagatgga aggcagatta
    39781 acggcgcaga ttacttagct gctccagtgc caggtaaagc gctcgctatt ttcggcgata
    39841 ccggcccctg cgatgccgca cttgacctgg ctaaaggtgt cgatgtcatg gtgcacgaag
    39901 cgacgctgga tataaccatg gaagccaaag ccaatagtcg cggtcatagc tctacacgcc
    39961 aggctgcgac actggcccgt gaggctggtg tcggtaagct aatcattacc cacgtcagct
    40021 cgcgctatga tgacaaaggt tgtcagcacc tgttacgtga atgtcggtca attttcccgg
    40081 cgactgaact ggcgaatgat ttcaccgtgt ttaacgttta actctgctta ttattaaaaa
    40141 cagggcgaaa cttgccctgt tatcgcaacc cgcgctacat atatccaccc acacgggcat
    40201 attgagcgat ttttgatatt tattcatctc cttacgcact atattcagag caataagttg
    40261 cctcttttta tcttcattca gcacaacctt tgcaactaaa aggagcatat gatggttaca
    40321 gttgtcagca attattgtca attatctcaa acccaactca gtcagacatt tgcagaaaaa
    40381 tttactgtga ccgaggaatt actgcagtct ttaaaaaaaa cagcgttatc cggagatgaa
    40441 gaaagtattg agttactgca taatattgcg ttaggttatg atgaatttgg aaaaaaagct
    40501 gaagatattc ttt
//
LOCUS       DAPGRN010000031        39970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPGRN010000031 DAPGRN010000000
VERSION     DAPGRN010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.031"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            216..1589
                     /gene="aroP"
                     /locus_tag="RHW33_001785"
     CDS             216..1589
                     /gene="aroP"
                     /locus_tag="RHW33_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV1645295.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            1748..3154
                     /locus_tag="RHW33_001786"
     CDS             1748..3154
                     /locus_tag="RHW33_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645296.1"
                     /translation="MNSSKLSVREKIGYGMGDAGCNIIFGAIMLFVNYFYTDIFGLAP
                     ALVGVLLLSVRVIDAVTDPIMGAIADRTQSKYGRFRPWLLWIAFPYALFSILMFTTPE
                     WSYSSKVIYAFVTYFLLSLTYTAINIPYCSLGGVITNDPQERVACQSYRFVMVGIATL
                     LLSLTLLPMVDLFGGGDKAKGYQLAMTVLAIIGMCMFLFCFATVRERIRPAVQTHDDM
                     KNDFKDVWKNDQWVRILLLTLCNVCPGFIRMAATMYYVTWVMGESTQFATLFISLGVV
                     GMMIGSMLAKVLTDRWCKLKVFFWTNIALAIFSCAFYFFNPHATVMIVVLYFLLNVLH
                     QIPSPLHWSLMADVDDYGEWKTGKRITGISFSGNLFFLKLGLAIAGAMVGFLLSWYGY
                     DAGAKAQSATAINGIVLLFTVIPGVGYLITAGVVRLLKVDRELMKRIQTDLEKRRTNY
                     RELNDYQEINAVETVRKA"
     gene            3154..4104
                     /locus_tag="RHW33_001787"
     CDS             3154..4104
                     /locus_tag="RHW33_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDV1645297.1"
                     /translation="MQDWPNPFIEQRADPFILRDGNHYYFIASVPEYDRLEIRRADSL
                     VGLRSATPVVVWRKPQSGPMSELIWAPELHHIAGRWYIYFAATHTQALDKLGMFQHRM
                     FALECCDADPLSGTWIEKGQVKTPFDTFALDATTFHHQGKQWYLWAQKAPDIAGNSNI
                     YLAEMENPWTIKGEPVMLSKPEYDWECRGFWVNEGPAVVMHGERLFISYSASATDENY
                     CMGLLWIDIDADPRVPENWHKSPRPVFSTSYENRQYGPGHNSFTQTPEGEDVLVYHAR
                     NYTEIEGDPLYDPNRHTRLKLIRWDESGMPDFGIPPAETY"
     gene            complement(4134..4988)
                     /gene="ampE"
                     /locus_tag="RHW33_001788"
     CDS             complement(4134..4988)
                     /gene="ampE"
                     /locus_tag="RHW33_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV1645298.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(4985..5536)
                     /gene="ampD"
                     /locus_tag="RHW33_001789"
     CDS             complement(4985..5536)
                     /gene="ampD"
                     /locus_tag="RHW33_001789"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV1645299.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            5624..6517
                     /gene="nadC"
                     /locus_tag="RHW33_001790"
     CDS             5624..6517
                     /gene="nadC"
                     /locus_tag="RHW33_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV1645300.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            6720..7160
                     /gene="ppdD"
                     /locus_tag="RHW33_001791"
     CDS             6720..7160
                     /gene="ppdD"
                     /locus_tag="RHW33_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV1645301.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            7170..8555
                     /gene="gspE"
                     /locus_tag="RHW33_001792"
     CDS             7170..8555
                     /gene="gspE"
                     /locus_tag="RHW33_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV1645302.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVAQGLTTFEE
                     LIRVLGMPHGE"
     gene            8545..9747
                     /gene="hofC"
                     /locus_tag="RHW33_001793"
     CDS             8545..9747
                     /gene="hofC"
                     /locus_tag="RHW33_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV1645303.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(9782..10825)
                     /gene="guaC"
                     /locus_tag="RHW33_001794"
     CDS             complement(9782..10825)
                     /gene="guaC"
                     /locus_tag="RHW33_001794"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV1645304.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVKNTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(10981..11025)
                     /locus_tag="RHW33_001795"
     CDS             complement(10981..11025)
                     /locus_tag="RHW33_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645305.1"
                     /translation="MIIKTLKMSARKRN"
     gene            11050..11670
                     /gene="coaE"
                     /locus_tag="RHW33_001796"
     CDS             11050..11670
                     /gene="coaE"
                     /locus_tag="RHW33_001796"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV1645306.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            11670..12413
                     /gene="zapD"
                     /locus_tag="RHW33_001797"
     CDS             11670..12413
                     /gene="zapD"
                     /locus_tag="RHW33_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV1645307.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            12423..12620
                     /gene="yacG"
                     /locus_tag="RHW33_001798"
     CDS             12423..12620
                     /gene="yacG"
                     /locus_tag="RHW33_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV1645308.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(12836..13225)
                     /gene="mutT"
                     /locus_tag="RHW33_001799"
     CDS             complement(12836..13225)
                     /gene="mutT"
                     /locus_tag="RHW33_001799"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV1645309.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGIIPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(13285..15990)
                     /gene="secA"
                     /locus_tag="RHW33_001800"
     CDS             complement(13285..15990)
                     /gene="secA"
                     /locus_tag="RHW33_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV1645310.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLESLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(16052..16564)
                     /gene="secM"
                     /locus_tag="RHW33_001801"
     CDS             complement(16052..16564)
                     /gene="secM"
                     /locus_tag="RHW33_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV1645311.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(16795..17712)
                     /gene="lpxC"
                     /locus_tag="RHW33_001802"
     CDS             complement(16795..17712)
                     /gene="lpxC"
                     /locus_tag="RHW33_001802"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV1645312.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(17813..18964)
                     /gene="ftsZ"
                     /locus_tag="RHW33_001803"
     CDS             complement(17813..18964)
                     /gene="ftsZ"
                     /locus_tag="RHW33_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV1645313.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(19025..20287)
                     /gene="ftsA"
                     /locus_tag="RHW33_001804"
     CDS             complement(19025..20287)
                     /gene="ftsA"
                     /locus_tag="RHW33_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV1645314.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(20284..21114)
                     /gene="ftsQ"
                     /locus_tag="RHW33_001805"
     CDS             complement(20284..21114)
                     /gene="ftsQ"
                     /locus_tag="RHW33_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV1645315.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLQPEESTQQQNQAQAEQQ
                     "
     gene            complement(21116..22036)
                     /gene="ddlB"
                     /locus_tag="RHW33_001806"
     CDS             complement(21116..22036)
                     /gene="ddlB"
                     /locus_tag="RHW33_001806"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV1645316.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(22029..23504)
                     /gene="murC"
                     /locus_tag="RHW33_001807"
     CDS             complement(22029..23504)
                     /gene="murC"
                     /locus_tag="RHW33_001807"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV1645317.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(23558..24625)
                     /gene="murG"
                     /locus_tag="RHW33_001808"
     CDS             complement(23558..24625)
                     /gene="murG"
                     /locus_tag="RHW33_001808"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV1645318.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(24622..25866)
                     /gene="ftsW"
                     /locus_tag="RHW33_001809"
     CDS             complement(24622..25866)
                     /gene="ftsW"
                     /locus_tag="RHW33_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV1645319.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(25866..27182)
                     /gene="murD"
                     /locus_tag="RHW33_001810"
     CDS             complement(25866..27182)
                     /gene="murD"
                     /locus_tag="RHW33_001810"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV1645320.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYLFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(27185..28267)
                     /gene="mraY"
                     /locus_tag="RHW33_001811"
     CDS             complement(27185..28267)
                     /gene="mraY"
                     /locus_tag="RHW33_001811"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV1645321.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(28261..29619)
                     /gene="murF"
                     /locus_tag="RHW33_001812"
     CDS             complement(28261..29619)
                     /gene="murF"
                     /locus_tag="RHW33_001812"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV1645322.1"
                     /translation="MISVTLSQLTDILNGELQGADITFDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSLDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(29616..31103)
                     /gene="murE"
                     /locus_tag="RHW33_001813"
     CDS             complement(29616..31103)
                     /gene="murE"
                     /locus_tag="RHW33_001813"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV1645323.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(31090..32856)
                     /gene="ftsI"
                     /locus_tag="RHW33_001814"
     CDS             complement(31090..32856)
                     /gene="ftsI"
                     /locus_tag="RHW33_001814"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV1645324.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(32872..33237)
                     /gene="ftsL"
                     /locus_tag="RHW33_001815"
     CDS             complement(32872..33237)
                     /gene="ftsL"
                     /locus_tag="RHW33_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV1645325.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(33234..34175)
                     /gene="rsmH"
                     /locus_tag="RHW33_001816"
     CDS             complement(33234..34175)
                     /gene="rsmH"
                     /locus_tag="RHW33_001816"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV1645326.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(34177..34635)
                     /gene="mraZ"
                     /locus_tag="RHW33_001817"
     CDS             complement(34177..34635)
                     /gene="mraZ"
                     /locus_tag="RHW33_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV1645327.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(35237..36241)
                     /gene="cra"
                     /locus_tag="RHW33_001818"
     CDS             complement(35237..36241)
                     /gene="cra"
                     /locus_tag="RHW33_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV1645328.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(36323..36409)
                     /locus_tag="RHW33_001819"
     CDS             complement(36323..36409)
                     /locus_tag="RHW33_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV1645329.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(36421..36912)
                     /gene="ilvN"
                     /locus_tag="RHW33_001820"
     CDS             complement(36421..36912)
                     /gene="ilvN"
                     /locus_tag="RHW33_001820"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV1645330.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(36915..38639)
                     /gene="ilvI"
                     /locus_tag="RHW33_001821"
     CDS             complement(36915..38639)
                     /gene="ilvI"
                     /locus_tag="RHW33_001821"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV1645331.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(38957..39901)
                     /gene="leuO"
                     /locus_tag="RHW33_001822"
     CDS             complement(38957..39901)
                     /gene="leuO"
                     /locus_tag="RHW33_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308107.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV1645332.1"
                     /translation="MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9589 a        11119 c         9792 g         9470 t
ORIGIN      
        1 agctttacaa acggtttctt tttaagcaac tcatcttcaa ccatgcataa agcgggtgca
       61 ttcgctgccg cataccatta ttcttgatct gacggaagtc tttttgtaac aattcaaact
      121 tctttgatgt aaacaaatta atacaacaaa cggaattgca aacttacaca cgcatcactg
      181 cgtagatcaa aaaaacaatc accgcacgag gtttcatgat ggaaggtcaa cagcacggcg
      241 agcagctaaa gcgcggcctt aaaaaccgcc atattcagct tatcgcgctg ggtggcgcga
      301 tagggacagg gttattcctg ggtagcgcct ccgtaataca gtccgcaggg ccagggatta
      361 tcctgggtta cgccattgct ggttttatcg cctttctgat catgcgtcag ctgggtgaaa
      421 tggtggtcga agaacctgtc gcaggctcct ttagccactt tgcttataaa tactggggca
      481 gctttgctgg cttcgcttct ggctggaact actgggtact gtacgtttta gttgccatgg
      541 cagagctgac tgctgtgggt aaatacattc agttctggta tccggaaatc ccaacctggg
      601 tttctgccgc cgtgttcttt gtggtgatta acgccatcaa cctgaccaac gtaaaagtgt
      661 ttggtgagat ggagttctgg tttgccatta tcaaagttat tgcggtagta gcgatgatca
      721 tcttcggcgg ctggctgctg ttcagtggta acggcggtcc gcaggcaagc gttagcaacc
      781 tgtgggatca gggtggtttc ctgccacacg gcttcaccgg gctggtgatg atgatggcga
      841 ttatcatgtt ctcgttcggt ggtctggaac tggtggggat caccgcagca gaagctgata
      901 acccggagca aagtatcccg aaagcgacta accaggttat ctaccgcatc ctgattttct
      961 atattggttc gttagccgtt ctgctctcac tgatgccgtg gacccgcgtt accgccgata
     1021 ccagtccgtt tgtgctgatc ttccacgagt taggcgatac ctttgtggcg aatgcgctga
     1081 acatcgtggt actgactgcg gcgctctccg tgtacaacag ctgcgtatat tgcaacagcc
     1141 gtatgctgtt tggtctggca caacagggta atgcgccaaa agcgctggcg tctgtcgata
     1201 aacgcggcgt accggttaac accattctgg tgtctgcgct ggttacagca ttgtgcgtat
     1261 tgattaacta tcttgctccg gaatccgcat ttggcctgtt aatggcactg gtggtatccg
     1321 cactggtgat caactgggcg atgatcagtc tggcgcatat gaagttccgt cgcgccaagc
     1381 aggaacaagg cgtggtaact cgcttccctg ctctgcttta tccgctgggt aactggatct
     1441 gcctgctgtt tatggcggcg gtactggtga ttatgctgat gaccccagga atggcgattt
     1501 cggtatacct gatcccggta tggctgatcg tgttaggtat cggctatctg tttaaagaga
     1561 aaaccgccaa agccgtaaaa gcgcattaat ctctctacgc cctcacccgt acagggtgag
     1621 ggttgttacc tgcctcacac ttttccgtgc aaagcgattt tatccatatc agcggcgtta
     1681 tcctgcaacg caatttatcc atagcgagcg aataattatc ctcatcactt gatggggagt
     1741 cttctctatg aatagcagta aattatcagt aagagaaaag atcggctacg ggatgggcga
     1801 cgcaggatgc aacatcatct ttggcgctat catgctgttt gttaactatt tttatacgga
     1861 tatttttggt ctggccccgg cactcgttgg cgtattactg ctctctgtac gggttatcga
     1921 tgccgtcact gaccctatta tgggggccat tgccgaccgc acccaaagca aatatggtcg
     1981 ttttcgcccc tggctattgt ggattgcctt cccctacgcg ctattcagca tcctgatgtt
     2041 taccacgcca gaatggagct atagcagcaa ggttatctat gcctttgtta cctatttcct
     2101 gctctccctg acctataccg ccattaacat tccttactgc tcgttaggtg gcgtgattac
     2161 taatgaccca caagagcgcg tggcctgcca gtcgtaccgt tttgtgatgg tgggcatcgc
     2221 tacccttctg ctctcgctga cgctattgcc gatggtggat ctatttggcg gcggcgacaa
     2281 agcgaaaggg tatcagctgg ctatgaccgt gctggcaatc atcggtatgt gtatgttttt
     2341 gttctgtttc gccacggttc gtgagcgcat tcgcccggcg gtacaaaccc atgacgacat
     2401 gaagaacgat tttaaagatg tctggaaaaa cgatcagtgg gtgcgcattc tgctgctcac
     2461 cttgtgcaac gtctgccctg gatttatccg catggcggca accatgtact atgtcacctg
     2521 ggtaatgggt gagagtactc agtttgcaac cctctttatt agcctcggcg ttgtcggaat
     2581 gatgatcggc agtatgttgg cgaaagtgtt aaccgaccgc tggtgcaaac tgaaagtctt
     2641 tttctggacc aacatcgcgc tggcaatttt ctcctgcgcc ttttatttct ttaatccgca
     2701 cgccacggtg atgattgtgg tgctctactt cctgctcaac gttctgcacc agatcccctc
     2761 cccgctgcac tggtcgctga tggcagacgt cgacgactac ggcgaatgga aaaccggtaa
     2821 acgcatcacc ggcatcagct tctccggtaa ccttttcttc ctcaaactgg gcctggccat
     2881 tgccggggcg atggtgggct ttctgctctc atggtacggc tacgatgccg gtgcgaaagc
     2941 ccaaagtgcc acggcgatta acggtattgt actgctcttt accgtcattc ctggggtggg
     3001 ctatctgatt accgcaggcg tcgtccgcct actgaaagta gaccgcgaac tgatgaaacg
     3061 catccagacc gatctggaaa aacgtcgcac caattaccgc gaactgaatg actatcagga
     3121 aataaacgct gtagaaacag taaggaaagc ctaatgcaag actggccgaa tccatttatc
     3181 gagcaacgcg ccgatccctt tatactgcgc gatggcaacc actattattt tatcgcctcg
     3241 gtgccggagt acgaccggct ggaaattcgc cgggcagact cgctcgtcgg gctgcgcagc
     3301 gccacgcctg tcgtggtgtg gcgtaaaccg caaagcggcc ccatgagcga acttatctgg
     3361 gcgccggagc tgcatcatat tgcaggtcgg tggtatatct attttgccgc cacccatact
     3421 caggcgctcg acaaactggg gatgtttcag caccggatgt ttgcccttga atgttgtgat
     3481 gccgatccgc tcagcggaac gtggattgaa aaaggacaag ttaaaacacc atttgatact
     3541 tttgccctgg atgccaccac ttttcaccat cagggtaaac agtggtattt gtgggcgcaa
     3601 aaagcaccgg atatcgccgg gaactccaat atttacctgg ccgaaatgga aaatccgtgg
     3661 acgatcaaag gcgagccggt gatgttgagc aaaccggagt atgactggga atgtcgcgga
     3721 ttctgggtca atgaaggtcc ggctgtcgtg atgcatggcg agcggttatt catcagctac
     3781 tcagccagcg ctaccgacga gaactactgc atggggttat tgtggataga catcgacgct
     3841 gacccgagag tgcctgagaa ctggcataaa tcgccgcgtc cggtcttttc gaccagctat
     3901 gaaaatcgcc agtatggccc cggacataac agttttaccc aaacgcccga gggtgaggat
     3961 gtgctggtct accacgcacg taattacacc gaaatagaag gcgatccact gtacgatcct
     4021 aatcgccata cgcgcttaaa attgattcgc tgggatgaaa gtgggatgcc tgactttggc
     4081 atccctcctg cggagactta ctgattacaa tgcccggtgt atatcgccgg gcactacacc
     4141 aacgccccgt aaatcgtcag tagcgcaatc accaccacga ccacgaacga ggttttcttc
     4201 gccattgaaa ccgctgcctt cggcgtctcc accttatcga catgcggttc acgcgccaga
     4261 gagaactgcg ccagacgcgt taacacctga tactgcgaag tatggaaatc ccccagcgaa
     4321 gcaaaccagg ccggtaacgc tttctcacca tgaccgatca aggcatatac cactcccgca
     4381 agacgaaccg gcacccaatc cagtacatga agcactgcat caatgccgga ctgtaaacga
     4441 tgatgcggcg tctggtatcg cgccagccag tattgccatg cacgcaagaa cgcatacccc
     4501 atcagcgtaa cgggtcccca ggttcccccc acaatcagcc agaacagcgg tgcaagataa
     4561 aaacgaaagt taatccacag caatgcattt tgcagctcac gcaaatactc acgttcgtcg
     4621 cagcctgccg ggacgccgtg aatcatggtg agttcgccag ccatcgtggc acgggcatgg
     4681 ctatcattac gtgaagcagc tgtcagataa gcatgataat gaagacgaac tttacctgcg
     4741 ccaatacaca gcaaaccaat cagcagccac accagcagcg tgggaacgtt gaacaatact
     4801 ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg caataatggt catgcctaac
     4861 gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg atgatcaagc
     4921 tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa ggttgtaaat
     4981 agcgtcatgt tgtctccttg ctgaccagcg cacgaaaccg tgcccaatca aatgcaggac
     5041 cgggatcggt tttccgatcc ggcgcaatat cacaatggcc cgtcatgtta ttagcgatat
     5101 ccggatagca atcaatcagt gcccgcgtaa ccgccgcaag ctgttgatac tgcgcatcgg
     5161 tatacgccag cgtatcggtg ccttcaagct caatcccaat agaaaaatca ttgcagcgtt
     5221 cgcgcccctg atactgagag actcccgcat gccatgcacg tttatcgaaa ggaacatact
     5281 ggactatttc accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa tgggcgatct
     5341 cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt gaataatgcg tcgatccacg
     5401 gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac caccagcagg gtgggtgttt
     5461 cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca accagccacc
     5521 cctgttctaa caacatgcag gatctcctta tatgtggtgc taatacccgg ttcagagtag
     5581 catgtttcta ccttatgatt cgttagctat ctggagtttt aacatgccgc ctcgccgcta
     5641 caaccctgac acccgacgtg acgagctgct ggaacgcatt aatctcgata tccccggcgc
     5701 ggtggcccag gcgctgcggg aagatttagg cggaacagtc gatgccaaca atgacattac
     5761 ggcaaaactt ttaccggaaa attctcgctc tcatgccacg gtgatcaccc gcgagaatgg
     5821 cgtattttgc ggcaaacgct gggttgaaga ggtgtttatt caactggcag gcgacgatgt
     5881 caccataatc tggcatgtgg atgacggcga tgtcatcaac gccaatcaac ccttgttcga
     5941 acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc actgcgctta attttgtgca
     6001 aaccctttca ggagttgcca gtaaggtacg ccactatgtc gaattactgg aaggcaccaa
     6061 cacgcagttg ttggatacgc gcaaaacctt acccggcctg cgttcagctc tgaaatacgc
     6121 ggtactttgc ggcggcggag cgaatcaccg tctggggctt tctgatgcct tcctgatcaa
     6181 agaaaaccat attattgcct ccggctcagt gcgccaggcg gtcgaaaaag cgtcctggct
     6241 gcatccggat gcgccagtag aagtcgaagt agagaatctg gaagaacttg atgaggccct
     6301 gaaagccgga gccgatatca tcatgctgga taacttcgaa acagaacaga tgcgcgaagc
     6361 cgtcaaacgc accaacggca aggcgctact ggaagtgtct ggcaacgtca ctgacaaaac
     6421 actgcgcgaa tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc taactaaaca
     6481 cgtacaagca ctcgaccttt caatgcgttt tcgctaatag ctgacagatt tatccgctcc
     6541 atactgggcg gataatctcc ccttccccaa ccttcttcgt aacgcctcgc aaatttcttg
     6601 tctttcgctg caacgaaata aaaaacctcg gaagcagctt tcccgtttcg ttttttgccc
     6661 ctcgcctgtg ctgatggcgt ttgccaaagt agcaccaacc aaatcaagga gcgaaacaga
     6721 tggacaagca acgcggtttt acacttatcg aactgatggt ggttattggc atcattgcca
     6781 ttttaagcgc cattggtatt cccgcttatc aaaactacct gcgcaaagcc gcactcaccg
     6841 acatgctaca aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg ctggaacatg
     6901 gtggattaga tacctgcgac ggtggcagca atggcattcc ctcgcctacc accacccgct
     6961 atgtttcagc catgagtgtg gcaaagggcg tggtgtcgct gaccgggcaa gaaagtctca
     7021 atgggctaag cgtcgtcatg acgccaggtt gggataacgc aaacggcgtc accggctggg
     7081 cgcgcaactg caatattcaa agtgacagcg cattgcagca agcctgcgaa gatgtcttcc
     7141 gctttgatga cgccaactaa ggagcggcaa tgaatattcc acagctcacg gccctgtgtc
     7201 tgcgttatca gggagtcttg ctggatgcca gcgaagaagt ggttcatgtt gcggtggtcg
     7261 atgccccctc acatgagttg ctggacgcat tgcatttcgc taccaccaaa cgtattgaga
     7321 tcacctgctg gacgcgccaa caaatggaag gtcacgccag tcgcacacaa cagacattgc
     7381 ccgtagctgt tcaggagaag catcagccca aagcagagtt gctgactcga acgttacaat
     7441 ctgcgctgga acaacgcgcg tctgatattc atatcgaacc agcggacaat gcctaccgca
     7501 tccgcttgcg tatcgacggc gtattgcatc ctttaccgga tgtttcaccg gatgccggag
     7561 tcgcattaac cgccagatta aaagtgctgg gaaacctgga tattgcggaa catcgcctgc
     7621 cgcaggacgg gcaattcact gtcgaactgg caggaaacgc cgtctcattt cgtattgcga
     7681 ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt gttacagcag gtgagccagg
     7741 cactggatgt caacacgctg ggaatgcagc cgttacaact ggcggacttt gctcatgcct
     7801 tgcaacaacc acagggactg gtgctggtaa ctggccctac cggcagcggc aaaacggtca
     7861 cgctttatag tgccctgcaa acgctgaata ccgctgacat taatatttgt agcgtcgaag
     7921 atccggttga gatccccata gccggactaa accagacgca aatccatccg cgtgccggac
     7981 tcacctttca gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc atcatgatcg
     8041 gagagatccg cgatggcgaa acggcagaga tcgctattaa agccgcgcaa accggtcacc
     8101 tggtgttgtc taccctacac accaattcca cctgcgaaac gctggtacgt ttacagcaaa
     8161 tgggagtcgc ccgctggatg ctctcatcag cgcttacgct ggtaatagcc cagcgtctgg
     8221 tacgtaaact ttgcccacat tgtcgccagc agcaagggga gcccatccat attccagaca
     8281 atgtatggcc gtcgccgctg ccccactggc aggcacctgg ttgtgtacat tgctaccacg
     8341 gtttttatgg tcgcacggcc ttatttgaag ttctgcccat aacaccggtc atacgtcagc
     8401 ttatttccgc taataccgac gttgaatcgc tggaaactca cgcccgacag gcgggtatgc
     8461 gtacgctttt tgaaaacggc tgcctcgccg tggcgcaagg cttaaccacc tttgaagagt
     8521 taatccgcgt actggggatg ccgcatggcg agtaagcaac tctggcgctg gcatggcata
     8581 accggcgacg gcaatgcgca agatgggatg ctatgggcag agagccgtac tttactgctt
     8641 atggcactac agcaacagat ggttacccca ctaagcctga agcgaatcgc catcaattct
     8701 gcgcagtggc ggggagataa aagcgcggaa gtcattcatc aactggcgac gctactcaaa
     8761 gcagggttaa cgctttctga agggctggct ctgctggcgg aacagcatcc cagtaagcaa
     8821 tggcaagcgt tgctgcaatc gctggcgcac gatctcgaac agggcattgc tttttccaat
     8881 gccttattac cctggtcaga ggtatttccg ccgctctatc aggcgatgat ccgcacgggt
     8941 gaactgaccg gtaagctgga tgaatgctgc tttgaactgg cgcgtcagca aaaagcccag
     9001 cgtcagttga ccgacaaagt gaaatcagcg ttacgttatc ccatcatcat tttagcgatg
     9061 gcaatcatgg tggttgtggc aatgctgcat tttgttctgc cggagtttgc cgctatctat
     9121 aagaccttca acaccccact accggcacta acgcagggga tcatgacgct ggcagacttt
     9181 agtggcgaat ggagctggct gctggtgttg ttcggctttc tgctggcgat agccaataag
     9241 ttgctgatgc gccgcccgac ctggcttatc gcgcggcaga aattgctgtt acgcatcccg
     9301 attatgggtt cactgatgcg gggacaaaaa ctcacgcaga tctttacgat tctggcgctg
     9361 actcaaagtg caggcattac ttttttacag ggcgtagaga gcgtcagaga aacaatgcgc
     9421 tgcccgtact gggtgcaact tctgacacaa atccagcacg atatcagtaa cggtcatccc
     9481 atctggctgg cgctaaaaaa tgccggtgag tttagcccgc tctgtttgca attagtgaga
     9541 acgggagagg cctccggctc gctggatctc atgttagaca acctcgccca tcatcatcgg
     9601 gataacacaa tggcgctggc ggataaccta gcagccttac tggaaccggc gttgctgatc
     9661 ataacgggag gaattatcgg tacgctggtg gtggcgatgt atctgccaat tttccattta
     9721 ggcgatgcga tgagtgggat gggataacgc tggcgtgttg ctccacacca gcgttggaag
     9781 attacaggtt gttgaagatg cggttttctt gttcctgcac acggataaat gtggtgcgct
     9841 tggtcagttc tttcaggcgt gaagccccaa cgtatgtaca agctgaacgc aggccgccca
     9901 aaatatcacg cgcggtattt ttaaccgggc ctcgcagcgg cagcttaacg gttttacctt
     9961 ctgctgcgcg atattccgca acgccgccaa cgtgacgttt catcgcagac tcggagctca
    10021 tgccgtagaa cagcataaat ttctcgccgt tctcctcaac gatgcgaccg ccgctctctt
    10081 cgtggcccgc cagcatgccg ccaagcatga cgaaatcggc accgccgcca aaagctttcg
    10141 cgacatcgcc cggcgtggtg caaccaccat cgctgacgat cattccgccc agaccgtgcg
    10201 cagcatcggc acattcgatt accgcagaaa gttgcggata accgacgcct gttttgacac
    10261 gagttgtaca aacagaacct gggccaatgc caactttaac gatatcggca ccggagagga
    10321 taagctcctc acacatttca ccagtcacta cgttgccagc acaaatggtt ttggtcggcc
    10381 acgcttcacg cgctttcgca acgaactgca cgaagtgttc ggaataacca ttcgccacat
    10441 caatacaaac gaagtttaat gccgggttca ggtcgagaat ctgtttagtt ttttcgaaat
    10501 ccgcatcaga cgtaccggta gaaaccatca catgtttcag cacatcagcg gaagaattgt
    10561 tgataaacgc ttgccactct tcgacagaat agtgtttatg cacagcagtc aaaatatcaa
    10621 aagaagccag cgcagaggcc atagaaaatg tgcctacggt gtccatattt gcggcgataa
    10681 tcggcacgcc ggaccagctc tgacctgaat gtttgaaggt gaattgacgt tccagttcaa
    10741 catcggaacg gcttttaaga gtggagcgtt tagggcggat gagaacgtct ttaaaaccta
    10801 acttcagatc ttcttcaata cgcatgtgcg gattcctggg gttaatggct ataagggtaa
    10861 gagcgcaact ccagtgacgc tatcatacgc actaatcaat gcggcgcaag actgcgaaat
    10921 tgtctttttt tacgctacaa tcccataaat ttacctggta aatagtgagc gaacgtttca
    10981 tcaatttcgc tttcgtgcgc tcatttttaa cgttttgata atcatgatga attcccggga
    11041 atataaatta tgaggtatat agttgcctta acgggaggca ttggcagtgg caagagtacc
    11101 gttgccaatg cgtttgctga tctcggaatt aacgtcattg atgccgatat tattgcgcgt
    11161 caggtggttg aaccaggcgc acctgcgcta catgccattg ctgatcactt tggcgctaac
    11221 atgattgctg ctgatggaac attgcagcgc cgggccttgc gcgagcggat cttcgccaac
    11281 ccggaagaga aaaactggct taacgccctg ctgcatccgc tgattcagca agagacgcaa
    11341 caccagatcc agcaagcaac ctccccctat gtactgtggg ttgtgccatt gctggtagaa
    11401 aactcactgt ataaaaaagc gaatcgagtg ctggtggtgg atgtcagccc agaaacgcaa
    11461 cttaagcgca caatgcagcg cgatgatgta actcgcgagc atgtcgaaca aatccttgct
    11521 gctcaggcaa cgcgcgaagc ccgccttgcc gtggcagatg acgtcattga taataacggc
    11581 gcaccggatg ctatcgcatc ggatgttgcc cgcctgcacg cacactattt gcagcttgcg
    11641 tcgcagtttg tctcacagga aaaaccgtaa tgcagaccca ggtccttttt gaacatccac
    11701 taaatgaaaa aatgcgtaca tggctgcgca ttgagttttt gattcagcaa ctcaccgtta
    11761 atttacccat cgttgaccac gctggcgcgc tgcatttctt ccgtaatgtc agtgaattac
    11821 tggatgtttt cgagcgcggc gaagtccgca ctgagctgtt gaaagaactt gaccggcagc
    11881 aacgtaaact ccagacctgg attggcgtgc ctggcgtgga ccagagccgt attgaagcat
    11941 taattcagca gttaaaagcg gcggggagcg tattaatctc cgcgccgcgt atcgggcaat
    12001 ttctacgtga agatcgtttg attgctctgg tgcgtcagcg actgagcatc ccaggcggct
    12061 gttgcagctt tgatttacca acattgcaca tttggctgca tctaccccag gcgcagcgcg
    12121 acagccaggt agaaacctgg attgccagcc tgaatccgct cacccaggca cttaccatgg
    12181 tgctggattt aattcgccag tcggccccct tccgtaaaca aaccagcctg aatggttttt
    12241 atcaggataa cggtggcgat gccgacttgc tgcgcctgaa tctgtcgctc gattcacagc
    12301 tttatccgca aatttccgga cataagagcc gttttgccat tcgttttatg ccgctggaca
    12361 ctgaaaacgg acaggtaccg gaacgtctgg atttcgaact ggcctgttgc taaggagtaa
    12421 aaatgtcaga aactattacg gtgaattgcc caacctgcgg gaaaacggtg gtgtggggtg
    12481 aaatcagccc gtttcggcca ttttgctcca aacgttgtca gttgatcgac ctcggagaat
    12541 gggctgctga agaaaaacga atccccagca gtggcgatct ttccgaaagc gatgactgga
    12601 gcgaagaacc aaagcagtga catttgtctg atgccgcacg taggcctgat aagacgcgga
    12661 cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc
    12721 tacaaaaaca aatttatcaa tgagttagaa atccctttag gcctgataag acgcgccagc
    12781 gtcgcatcag gcatcgtgtg cgaatgtcgg atgcggcgaa aacgccttat ctgacctaca
    12841 gacgtttaag cttcgcaatt accggttcat tggctggcgg aaaatcatcg gcattaagac
    12901 cgaccagcga catccactta ccgggttgcc cttctttacc ccacggctcc ccttcccagc
    12961 tttcgaccag ccagaaccac agagttatat gcctgtccgg gaattcatat tccagttttt
    13021 caaatagcga aaaatgttgt gggataatcc cgacttcttc ctgaagttca cgcaccaccg
    13081 cctgttccgg cgtttcaccc atttcaattt taccgccggg aaactccagt ttattcgcca
    13141 tgtgcgcatc tgctgcgcga cgcgttataa agatttcatt gttctcgttg cgaataatac
    13201 ctaccgcaat ttgcagcttt ttcattgtct taaacctata aaaaaggcgc agaatcctgc
    13261 gccttttact tcaactgtta gcttttattg caggcgacca tggcactgct tgtatttttt
    13321 accagaaccg cacgggcaag gatcgttacg tcctactttg cgttcaccgg tttgcgccgc
    13381 cagtgcagct gcggctgcag agtcgtcatc ctgatggcta agctgctgca tttgcgctaa
    13441 acgctcggct tccatacggc gctgttgttc cagctcctca acctcttcag gcatacgtac
    13501 ctggactttg ctcaacgtgc tgataacttc atattttaac gactccagca tcgctgcaaa
    13561 catggagaac gattcacgtt tgtattcctg cttcggatct ttctgtgcgt agccacgcag
    13621 gtggataccc tgacgcagat agtccatcgc tgccaggtgc tctttccaca gggagtcaag
    13681 cgtttgcagc atgacgcctt tctcgaagtg acgcatcatc tcagcaccaa ccacttcttc
    13741 tttacgctga tacacttcga tggactgcgc cagaatgcgc tcacgcagcg tctcttcatg
    13801 cagttctggt tctttatcca gccactcggc aattggtaaa tcgaggtcga aatcgttctt
    13861 cagacgttcc tgcagccccg gaatatccca catttcttcc agcgactgtg gcggaatgta
    13921 ggcatcaatg gtggctttga acacatcttc acgaatgctg ttgatggttt cgctcacatc
    13981 gctgacatcc agcagttcgt tacgctggga gtaaatggcg cgacgctgat cgttagccac
    14041 gtcatcatat tccagcagtt gcttacgaat gtcgaagtta cgactttcaa ctttacgctg
    14101 ggcgttggca atcgctttag tcacccacgg gtgttcaatg gcttcgcctg gcttcatacc
    14161 cagtttacgc atcattccgg atactcggtc ggaagcaaaa atacgcatca gcgcatcttc
    14221 catcgacagg tagaaacggg aagaaccagc atccccctga cgaccagaac gaccgcgcaa
    14281 ctggttatcg atacgacggg attcgtgacg ctcggtaccg ataatatgca ggccacctgc
    14341 tgccagtacc gcatcgtgac gtacctgcca gtcggcttta attttttcaa tttgctctgc
    14401 ggtcggattt tccagcgcgg caacttctgc ctgccagcta ccaccgagca caatatctgt
    14461 accacgaccc gccatgttgg tcgcgatagt caccgcagcc ggataacctg cctgagcaac
    14521 aatcgccgct tcgttagcgt ggaatttggc gttcaggaca ttgtgcttaa taccggcttt
    14581 ggtcagttcg tttgacacca actccgattt ttcgatggag atagtaccca ccagcaccgg
    14641 ttggcctttc gcagtacgtt ctttgatatc ttcaatgatc gcctgaattt tttccgcttc
    14701 agtcatgtag accaggtccg gcagatcttt acgaatcatt ggacggttgg tcggaacaac
    14761 gacggtatcc agtttgtaga tggagctaaa ttcgaaagct tcggtatcag cagtaccggt
    14821 catccccgcc agtttttcat acagacggaa gtagttctgg aaggtgatcg aagccagcgt
    14881 ttggttttcg ttctggatct gcacaccttc tttcgcttcc acagcctggt gcagaccatc
    14941 ggaccagcga cggccctgca tggtacgacc ggtgtgttcg tcaacgatga taacttcacc
    15001 atctttaacg atgtagtcga cgtcacgggt aaacagcgca tgagcgcgca gcgccgccgt
    15061 tacgtggtgc atcagcatga tgttggccgg agagtacaga gactcccctt catccatgat
    15121 gccctctttc accagcagtt cttcaatcag caccagacca cgttcggtca ggttcacctg
    15181 gcgagatttt tcgtccaccg agaagtggcc ttcgccctgg aaggtttcgg agtcttcttt
    15241 ttcctgacgg atcaggtgcg gaataatttt attcacgcgt ttatacattt ccgagctgtc
    15301 ttctgccggg ccggaaatga tcagcggtgt acgcgcttca tcgatcagga tggagtccac
    15361 ttcgtccacc agcgcatagt gcagtttacg ctgtacacgt tcttcagggc tgaacgccat
    15421 attgtcgcgc aggtagtcaa agccgtattc gttgttcgta ccgtaagtga tgtcagctgc
    15481 gtaagcttcg cgctttgccg gtgctggcat gcccggcagg ttgataccga cagtcaggcc
    15541 aaggaattca aacagcggac ggttgttttc ggcgtcacgt tgcgccaggt agtcgttgac
    15601 ggtaactaca tgcacgcctt taccggttag tgcgttcagg taagcaggca gcgttgcggt
    15661 cagggtttta ccttcaccgg tacgcatttc ggcgatgcag cgttcgttaa gaaccatacc
    15721 gccgagtaac tgaacgtcga agtgacgcat accaaagacg cgcttacttg cctcacgtac
    15781 cacggcgaaa gcttccggga tcagactttc cagcacttcg cctttttcca gacgtgcacg
    15841 aaactctgcg gttttccctt tcagttcttc gtcggagagt ttttccatct ccggttccat
    15901 ggcattgatg atgttgacca ctttgcgcat ccggcgcagg gtgcgatcgt tacgactacc
    15961 gaaaacttta gttaataatt tgattagcat aataaaatct caaacgcccc gcgttgcgga
    16021 gttaataaaa tgaagtaaag gtttattgtt gttagctgag gcgttgaggg ccagcacgga
    16081 tgccttgcgc ctggcttatc cagacgggcg tgctgaattt tgcttgtggg gtaaaatgcg
    16141 cataatcaat gcgataaccc ttttcagacg gcgtgccttc ctgggtcagc agcgcgctga
    16201 gcgtatccag taatgcaaga tgttgcgcct gaagaggcaa agattcttca gcaacgggca
    16261 gcgtttgcgg tgccattgcg aaggaaagat gacggattac cgtgcgaatg gcatgttgat
    16321 gccagtaatc aacggaatag ttcgaattcg ggcggcgcgt gttcgcttcc agcaaggcca
    16381 attgaccaaa gttaactttg gctgaaggct cgtggttgcg ggttgtcgct tttgcgggcg
    16441 cgtttggttc ggcggcgttg ctgagcgcag gcaaacctaa actcgccgcg accatcccta
    16501 acaagagatg cggccagaag tagcgtttac caaactgtcg ccagcgcgtc agtattccac
    16561 tcacgttatt gccatcccga tcgtattcaa acgtatttag cgcacaaatc ttatcattaa
    16621 agcctgaagt ccacagcacg aatgaagata agttgtgcgt aaaagtgctt aagaaaacag
    16681 cgttcgcacc gttttttata gctcttataa aattcaaaaa aaaacgactg gttcacctgg
    16741 ccggagagag tgccagattt gccagtcgaa ttttatacga cagtataaat gtcgttatgc
    16801 cagtacggct gaaggcgctt tgaaggccaa cggcagttct gcgtcgtcct ggaaggtcac
    16861 atattcccag gcttcctgtt tcgccaggac agcctgcagc agtttgttat tcagtgcatg
    16921 accggattta taagcggtaa atgcaccaat aatattgtga ccacacatga acaagtcacc
    16981 gatcgcatcg agcattttgt gacgcacaaa ttcgtcttca aaacgcaggc cgtcttcgtt
    17041 cagtacgcga taatcgtcaa caacgatggc acaatcgaag ctgccgccca ggcacaaacc
    17101 acgggactgc agatattcga tatcacgcat gaaaccgaac gtacgcgcac ggctgatctg
    17161 gcgcataaac gcatcagcgg agaagttcat cgcatagcgc tggttgctgg aatcaatagc
    17221 cggatggtta aaatcgatgg tgaaatccag cgaaaaacca ttgtacggct taaattcagc
    17281 ccacttatcg ccatcttcga cacgaacagt ctctttgatg cgaacaaatt ttttggcgca
    17341 gttcaactcg tcgataccgg cgtcaagcag caggtataca aacggagcgg cgctgccgtc
    17401 catgatcggg atttccggcg cgttaacttc gataacaatg ttatcgatgc ccaggcccgc
    17461 gagagcagca ttgaggtgct ctacggttga aatccgtaca tcatgctcgt tgaccagaca
    17521 cgtacagagc atggtatcac gcacagattt ggcatcggcc gggaaatcta ccggtggatt
    17581 caagtcggtg cgacgataga tgaccccggt gttggccggc gcagggcgta acgtcagggt
    17641 gactttcttg ccggtatgta aaccgacacc cgtcgcctga acgatacgtt taagtgtcct
    17701 ttgtttgatc atcgtattat ctcgccaaat tacctatcca accgaagtgt actatacatt
    17761 cggcgggcca gtttagcaca aagagcctcg aaacccaaat tccagtcaat tcttaatcag
    17821 cttgcttacg caggaatgct gggatatcca gataatccgg ctctttcgca gtttgcggcg
    17881 cattgtcatt cacgacttta gcaaccggct tctgctcctg ggtcagcgga gccatcccat
    17941 gctgctggta gcgatccatc actggctgct gaacctgctt attggtcacc agagtgattt
    18001 caggacgttt gtccatgccg atacctgtcg caacaacagt tacgcgcagc tcgtcattca
    18061 tatccgggtc aagagaagta ccgataacca cggtcgcgtt gtcggaagca aatgcacgga
    18121 tggtgttacc taccgtttcg aactcatcca gacgcaggtc gaagcccgct gtgatgttaa
    18181 ccagcacgcc gcgtgcgcca gacaggtcga tatcttccag cagcggagaa gagatagcca
    18241 tttcagcagc ttcttccgca cggtcttcac cgcttgccac gccagaaccc atcattgcgt
    18301 agcccatttc agacattacg gtgcgtacgt ctgcaaagtc cacgttcatc aaacccggac
    18361 gagtaatcag ttcggcgata ccttgcacag cgcctttcag tacatcgttc gctgcgccaa
    18421 acgcatccag cagggagata ccgcggccca gaactttcag cagtttgtcg ttcgggatag
    18481 tgatcagaga gtccacatgc ttggacagtt cagtgatccc ctgctcggcg aatgccatac
    18541 gcttcttgcc ttcaaagttg aaaggcttag tgacgacagc aacggtcagg atacccaaat
    18601 cttttgccac ttcagcgacg actggtgctg cacctgtacc ggtaccacca cccatacccg
    18661 cagcaataaa gaccatgtct gcaccttcca gcgccgcacg cagtgcatcg cgatcctcat
    18721 cagccgcatt gcggccaact tctggattag cgccagcgcc caatcctttg gtgataccgc
    18781 taccgatttg aatcgtctgt ccaaccgctg ttttacgcag cgcttgtgca tcggtattta
    18841 ccgcgaagaa ttcaacacct tcaatgcgct cgcgcaccat gtgttcaaca gcattaccgc
    18901 cgccgccgcc gacgccgatg actttaatca ccgcgtcatt ggtaagttcc attggttcaa
    18961 acatagtttc tctccgattt gtgcctgtcg cctgaggccg taatcatcgt cggcctcata
    19021 aaaattaaaa ctcttttcgc agccaactat tgagtcgctt gatccacgag ccaactgatg
    19081 ctgtaacacg tttttctact tcagcttcac cgttaagatg tgactctttc ccatagtgaa
    19141 gcaatcccac cgccgtcgaa taatacggct cctgagcata atccgttaaa ccggtaatgt
    19201 tcagcggcgc gccgatacgc acttgcgtat gaaacacgcg ctgagcacag gctgcaagac
    19261 cttcgatctg cgctgcgcca ccggttaata caatgcctgc cgccaggtga tgtttaaccc
    19321 cttgttggcg aagcttttcc tgcaactgca atatctcttc gttgaccagg ttgagcagct
    19381 cggtatagcg cggctcgatc acctctgcca gggtctgacg ttgcagactc cgcggcggac
    19441 gaccacctac gctcggcact tccacgctct catcttttcc aacgatggaa cccagcgcac
    19501 aaccgtggcg aactttaatc gcttcggcgt cgcttggcgg cgtgccaaag gcgtaagcga
    19561 tatcactggt cacgacattg ccagcataag gaattacctt agtgtggcgc aatgccccac
    19621 cggtataaac ggcgatatcc attgtaccac caccgatatc gacgacgcag acacccagtt
    19681 cacgttcatc ttccgtcaat accgaataac ttgatgccag accggcaaat atcagttggt
    19741 caactttcag cccacaacgt tcaaccgctt tgacgatgtt tttcgccata tcgttgtgac
    19801 atgtgatcag gtgcactttt gcctgcatcc gcacgcccga aagtcctacc ggattcttga
    19861 tcccttcctg atagtcaatc gcatactctt gcgggatcac atgcagcaca cgatgctcat
    19921 cgcgcacacg caccgatttc gcggtatgga cgacgttttc cacatcttct tgcgtcactt
    19981 cttcttcaga aataggcacc ataccaattt cattctggca gctgatgtgc ttaccagaaa
    20041 gcgccagata taccgatgag atctgacaat ctgccatcaa ttctgcctgg tcaatggcac
    20101 gttgtacgca cttgaccacg gattcgaggt cgttcacccc gcctttatcc ataccacgcg
    20161 acgggcagct gcccacgcca atgatattga ccataccgtc gggcagaact tcccctacta
    20221 aagcggcaac cttcgcggta ccaatctcca gtcctactac cagttttctg tccgtcgcct
    20281 tgatcattgt tgttctgcct gtgcctgatt ttgttgctga gtagattcct ctggctgcaa
    20341 tggcgcccag cctactgccg ctccagagtc ataacgcaaa tcaacgtagc taatccgttt
    20401 gccatcggtt tgcgcctgct gctgtaaaac cggataaagt tctacaaagc gagccaaacg
    20461 tttcatcgta tcgccccggc caagattgag cttaatatcg ttattcagcg tcaactgcca
    20521 ggaacgccgc gcggtcatcg ccgcttcctt cagagtaaat ctgtcctttg ccagcatctg
    20581 ccccatttcg cgatagccct gcaacacttc attggcgctg ccttccgggc catacagcat
    20641 tggaagcacc tgcttgctgg tgcgatctgg cggcacgctg aaggtatttc cttccgcgtc
    20701 taccatgtgt tgatcattcc accgcgcaat cggcacatat tcaaccagat gaatcttcaa
    20761 ttcatcaggc cactgctttc tgacgctcac ctgcttaatc cacggcaggc gttgttctat
    20821 ttgcgtctgg atgatgttga catcctgggt cataaaggta cccggctcac ccaatgccag
    20881 gatcgactgc cggatatcgt cattacgcgt gtaatggcgt tcaccggtca acaccagctt
    20941 tgagagcggc aggcgttgcg catcttccat ccagcccaac acgacccagc cgctcaccaa
    21001 cactgtcgtt aaaacggtca gcaggaaaag gatccccgcc agacgcgttc cattattgcg
    21061 gcgagaagaa acctcttctt cgctgtttcg cgtgttcaga gcagcctgcg acatattagt
    21121 ccgccagttc cagaattcgt actaccaact gcgagaagct catacctgcc tgacgtgccg
    21181 ccatcggcac caggctgtgg ctggtcatac ccggtgaggt attggcttcc agcagataaa
    21241 actgtccatc gctgtccagc ataacgtcaa tacgtcccca tcctttgcaa cctaacgtcg
    21301 tccatgcttt cagcactaat gcctgcaaat tggcctcttg tgacgcttcc agaccagcgg
    21361 ggcagaaata ctgtgtctca tcagagagat acttcgcctc ataatcatag aaggttccgg
    21421 ccggttgaat acgtattgac ggtaaaattt cttcaccgag tatcgcaacc gtgaactccg
    21481 gcccacttag ccatttttca atcaatactt cttcatcgtg ctgaaatgcc aatcttaatg
    21541 catcttgtag agcattttct gctaccactt ttgacattcc cacactggaa ccttcgcggc
    21601 tcggcttaac gataaccggc aaacccagag cagaaatttc tgctaactgc ttatcgctaa
    21661 ggcctttttc aaactctgcg cgggttaacg ctacccacgg cgcgaccggt aaaccagcac
    21721 cttgccacag cagtttgctg cgtagtttat ccattgaaag cgcagatgcc atcactccgc
    21781 tgccggtata aggcaagccc atcagctcga gcatgccctg cagcgtacca tcttcaccgc
    21841 cgcgaccgtg tagcgcgata aacactttct gaaagcccat cgactttagt tgcgtcacgt
    21901 cgacttcttt cgggtcgaca ggatacgcat caataccgcc ttcacgcagc ccggctaaca
    21961 ccgctgcgcc agaattcaga gaaacttccc gctcagcgga ggtcccaccc aacaggaccg
    22021 cgattttatc agtcatgttg ttcttcctcc ggagtttgcg gcttcagttt gatttcagct
    22081 aaagaacggg caatttttcc gatattacca gccccctgaa cgagaatcag gtcgttaccg
    22141 gttaataccg gtgccagcat ctcggctacc tgcgccggat ccggcaccag aatgggatct
    22201 attttcccac gtccacgaat tgtgcgacac agcgaacggc tgtccgctcc cggaattggc
    22261 gcttcgccag ccggatacac ttccagcatc aacagggtat caacctgcgt cagcacattg
    22321 gcgaaatcat catacaggtc gcgcgtacgg gtaaaacggt gcggctgaaa cagcattacc
    22381 aggtttttat ccggccagcc tgcacgcgct gctttaatag tggcgtccac ttccgtcggg
    22441 tggtggccgt agtcatcgac cagcatcgcc gtaccgcttt taccattcac tggctccagc
    22501 gggaattcac cgaggaaatc gaaacggcgc cccgtcccct ggaagctctc cagcgcacgc
    22561 aaaatagcct cgtcgtcaat gccctcttcc gtagcaaccg caaccgcagc tgcggcgttc
    22621 agcgcgttat gacggcctgg cgcattcagg gtgacgcgca tcggctcttt gtcctggcgc
    22681 agcagcgtaa agtgcccctg cgggccaatc tgctgataat cttctacacg cacgtcggca
    22741 tcttcgctga agccgtaagt cgtggtctga cgtcccacac gcggtaacaa ttcgcggatc
    22801 accggatcat caacacacat caccgcacga ccgtaaaacg gcaggttgtg cagaaaatta
    22861 ataaaagtct gttttaaatt ctcaaagtcg ccctggtagg tatccatgtg gtcggcttcg
    22921 atattggtga caatcgccac catcggttgc agatgcagga acgatgcatc actctcatct
    22981 gcttcggcaa tcaggtaccg accatgcccc aaacgcgcat gaacccccgc ggcttttacc
    23041 agcccgccgt taacgaaggt tgggtcgagc cccgcttctg cgtagatgct ggagaccatc
    23101 gcggtagttg tcgttttgcc gtgcgttcct gcaatggcga tgccatgacg aaaacgcatt
    23161 aactcagcca gcatttcggc acgacggatc accggaatac gcgcttcatg agctgcgaca
    23221 atttccgggt tatcggcaga aatagcgctg gaaacaacga ccacgctggc atcacgtacg
    23281 ttttccgggc gatggttgaa ataaatcgtc gctcccagat tcattaactg ctgcgtgacc
    23341 ggatttggcg ctaaatcgga accactgatc tgataacctt cattggccag aacttcggca
    23401 ataccgccca taccggcacc accgatgccg acaaaatgta tgtgccgaac gcgacgcatt
    23461 tcgggcacga tggaacgcag ttttgccaat tgttgtgtat tcattcttta cgccattaac
    23521 ttcttaaatt catacgatgc aaaaggcatc gctacaatta cgcccgggca gcccggctca
    23581 cttcatttgc cactcgctcg gtggcatccg gaatggatgc agcgcgggcg cgttctgcca
    23641 tggttaataa ggtttctcgc gaccacccgg ccagggtgtt ggcgacagca tccacgctaa
    23701 gctgtggctg ctcgataatt ttggctgcgc ccgctttttc cagcggtagc gcattccagt
    23761 attgctggcg gtctttatgt tgaaacggca caaacaacgc cggtagtcct gccgcggcga
    23821 tttcactcac cgttaacgca ccggagcggc aaacgacgac atccgcccac gcatacgccg
    23881 ccgccatatc atcaataaat tccgtcactt tatgctgcgg ttgccccgct tcggcatacg
    23941 cctgttcaac ggattgttgc gaacctttgc cgctctgatg ccagatagtg actgaatcac
    24001 ccagtttcgc agcaacctgc ggcattgtct ggttaagaat gcgtgcgccc tgggaaccac
    24061 ccactaccag cacacgaacc ggaccttcac gtccagccaa acgttgctgc ggcaacggta
    24121 gcgccaacac atcggtacgc accgggttac ccaccacttc cgcattgggg aaagcacctg
    24181 gaaacgcctg catcactttg gtggcaatct tcgccagcca tttattggtt aagcccgcaa
    24241 taccgttttg ttcatgaagt acaaccggaa tgcctaacga ccacgcggcc agaccaccag
    24301 gacctgatac gtagccgccc ataccgagca ccacgtcagg tttgtacgct ttcataatcg
    24361 cccgcgcctg acgccaggca ttgaagatac gcagcggcgc agctatcagt gcttttatac
    24421 cttttccacg cagaccagag atacgaatga aatcaatttc gatgccatgt tttggcacta
    24481 agtccgcttc catacggtcg gcagtcccca gccagcgaac ttgccaaccc tgagccatta
    24541 gatggtgcgc aaccgccagt cccgggaata catgtccacc ggttccgcct gccatcacca
    24601 ttaatcgctt tccttgacca ctcatcgtga acctcgtaca aacgcctgcg ctttctccag
    24661 acgcgtttca taatcaatac gcaacagcat catgatggct gtcgacataa tcagtaagct
    24721 cgaaccaccg taactgatca gcggcaatgt cagacctttg gtcggtaaca tccccgccgc
    24781 cgcgcctacg ttaactagcg cctggaagct aaaccagatg ccaatagaac aggcgagaaa
    24841 accggaaaaa cggtggtcaa tttctaatgc tttacggcca atcgacatcg cgcgaaaagc
    24901 gacgaagaat accattaaaa gtgccagcac cacaccgaca taccccagtt cttcgccgat
    24961 aatggcgaaa ataaagtcag tgtgcgcttc cggcagatac tccagttttt gtaccgagtt
    25021 acctaaacct tgcccccaaa gttcgccgcg accaaacgcc atcagcgatt gcgttaactg
    25081 atagccgctg ccaaagggat cttcccacgg gttccagaat gcggtaacac ggcggatacg
    25141 gtacggttcg gcgaggatca gcaacacaac cgctgaaatg cccataccga taatggcaat
    25201 gaactgccac aatttcgctc ccgccaggaa caacatcgcc agcgtagtca caaacaacac
    25261 caccaccgta ccaaggtctg gctgtgccag cagtaacact gccaacacca gaatcacgcc
    25321 catcggtttc aggaagccgc gcaggttatt acgtacttcg tcgcctttac gcaccagata
    25381 gttggcgata tagcaaaaca gcgacagttt tgtcagctcc gcaggctgga tacgcagcaa
    25441 accgagatcg atccaacgcg atgccccttt aaccgagctc cccactacca ggacgatcat
    25501 cagcaggatg atagatccga gcagcatcgt ggcactgtag cgttgccaga actccatcgg
    25561 cagacgcagc gtaatgatcg ccagaataaa cgccaaaatc agatagacgc catcacgctt
    25621 cgcgaagaag aacggatcgt tggttaagcg ttgccctatg ggcattgacg ccgaggtcac
    25681 catgataaag ccaatcgccg cgaggccgaa ggtcagccac agtaaggtgc gatcgtacat
    25741 gatcaggctg tcggtatctt tttcccgcga gcccatcacc cagcccttta gcgccgtgga
    25801 gatccagacc aggatactga atcctggcag gcgcggcatt ttcaggcgag ggagagataa
    25861 acgcatcaac ctaactcctt cgccagacgg gcaaactcat tgcctcgttg ttcaaagttc
    25921 ttgaactgat caaggctggc acaggccggg gagagcagaa ccatatcgcc cggctgaaca
    25981 cgcggagcca gcaagcgcat cgcctgttcc atagtttcgg tttgttctgc cacttccggg
    26041 cgtagcgccg ccagctgcgc gccgtcacga ccgaaacaat acagacgtac gttatcgcca
    26101 ttcaggtaac gcgctagtgg gctaaagtcc gccgatttac catcgccgcc cagcaacaaa
    26161 tgcagcgtgc cgtctacgtg caggccattc agcgccgctt ccgtactgcc gacgttggtc
    26221 gcttttgaat cgttaatcca acgtacgccg ttatgctcca gcacaacttc aaagcgatgc
    26281 ggcagaccag tgaatgtggt taatgctttc aggctgctgg cgcgcggcaa accagctgcg
    26341 tctgccagtg ccagtgctgc gagcgcattg gtgtagttat gctgtccgga aagtttcatc
    26401 tctttcacgt tcagcacttt ctcgccttta acccgcagcc aggtttcgcc ctgctgatga
    26461 ttcaggtgat agtcacccat gttgacgcca aagctgacgc agcgttcatc cgcaccgcga
    26521 atcggcattg ttaaggcatc atcagcatta accacgcaaa ctttcgcgtt ttcgtaaatg
    26581 cgcagttttg ctgcacgata ctgttgtaaa ccaaacagat agcgatccat atgatcttca
    26641 gtcacgttca gaatggtcgc tgctaccgcc tgtaagctgg aggtggtttc cagctggaag
    26701 ctcgacagtt ccagcacgta cagttcacac tcatcatcca gtagcatcaa cgcaggcagg
    26761 ccaatattgc cacccacacc aacgttaacc cccgccgctt tcgccatttc acccactagc
    26821 gtggtgaccg tacttttgcc gttagaaccg gtaatcgcca caatcggtgc ttgtgcttcg
    26881 cgacagaaca gctcaatatc gccaacgatt tcgattccgg catcagcggc agcgcttaag
    26941 gatggatgcg ccagtgcaat accgggactg gcgacaatca gatctgccgc catcagccat
    27001 tcatcattca gactgcccgt gtggcgttct acggcttcgg gtaatttatc caggccaggc
    27061 ggtgtcatac gcgtatccat aacgcgcggc gtcacaccgc gagcgaggaa aaagtccacg
    27121 caggaaagcc cggtgaggcc caggccgata atgacaacat ttttaccctg gtaatcagcc
    27181 atgattaacg taccttcagc gttgccagac caatcaggac cagcatcagc gaaataatcc
    27241 agaaacgcac aatgacgcgc ggttccggcc agcctttcag ttcatagtgg tgatgaatcg
    27301 gtgccatgcg gaaaatacgt tgtccgcgca gtttaaagga gccgacctgc aggatgacag
    27361 aaagcgtttc taccacgaac acgcccccca taatcaccag caggaattcc tgacgaagca
    27421 gtacggcgat aatgcctaac gcaccaccta acgccagcga acctacatcg cccataaaga
    27481 cctgcgccgg ataggtgtta aaccacagga agcccagtcc tgccccgact atcgcggtac
    27541 agacaataac cagttccccg gcgtgtcgca gatacggtat gtgtaaatag ctggcaaagt
    27601 tcatgttgcc ggtcgcccac gccaccagcg caaaaccacc ggcgacaaat acggtcggca
    27661 taattgccag accgtcaaga ccatcggtca ggtttaccgc gttgccagta cccacaatga
    27721 cgaagtaagc cagcagaatg tagaacagcc ccagttgcgg catcacatct ttaaagaatg
    27781 ggaccaccag ctgcgttgcg ggcgtgtctt tgccaacaag gtacagcgcg aaggcgacac
    27841 ccagcgcaat gaccgacatc cagaaatact tccaacgagc gatcaaccct ttggtgtctt
    27901 tacgcactac tttgcgataa tcatcaacaa agccaataac accgtaacct accagcacca
    27961 ccaacacgca ccagacgtac ggattggacg ggtaagccca cagcagtacg gagatcacaa
    28021 tcgccgtcag gatcataatc ccgcccatgg tcggcgtacc gcgcttgctg aagtgtgatt
    28081 caggaccgtc gttacgcacc acctgaccaa aggaaagttt ttgcaaatga gcaatcatac
    28141 gcgggcccat ccacaatgag atgaacagcg cggtcagcag gctgacgatg gcgcgaaacg
    28201 tcagatagga aaagacgtta aagccggaat aatatttgac caaatgttcg gccagccaaa
    28261 ctaacatgtc ccattctcct gtaaagcgcg tactacctct tccatggcgg cactacgtga
    28321 acccttaact aaaatcgtaa ttacctgttg ctcagcaatc agtgatttaa gacgcgcgat
    28381 aagcgctgtt ttatctgcaa aatgttcgcc aacgccgctg gcggtgctga tagcatggct
    28441 ttgtttaccc acgcttaaca cacggtcaat accagcagct tttgccgcct cgcccacctg
    28501 tacatggcag gcttcgcttt cagcgcccag ttccgccata tcgcccacca ccagcacgcg
    28561 gtagcccggc atttcagcca gtacctggac tgctgcggtc attgaaccga cattggcgtt
    28621 gtaggagtcg tcgagcagca actggttttc tgccagttgg atggggaaca gacggcctgg
    28681 aacagctttc agatttgcca gccccgcttt gatagcatca agcgttgcgc ccacggacat
    28741 ggagagcgca gcggctgcca gcgcattcgc aatattgtga cgccccggca acggcagcag
    28801 aacatccagg ctaccggttg gggtttgcag ggtaaattcc gtaccgtgcg aagtcacatg
    28861 gatattggtg gcggtgaaat cgctgttggc ggcattaggt gagaaacgcc acactttgcg
    28921 tgagccaatt acgctctgcc agttcagcca gtcgttgttg tcggcgttca taatggcgat
    28981 accgttttcc ggcaggccgc taaagatttc acctttcgct ttcgcgacac ccgcaagcga
    29041 gccaaaacct tccagatgcg ccgctgccag gttgttgacc agcgccgctt ccgggcgagt
    29101 cagactcaca gtccaggcaa tttcgccctg atggttcgcg ccaagttcaa taactgcgta
    29161 atcgtattcc ggcgttaagc gcaacagcgt catcggtaca ccgatgtcgt tgttgagatt
    29221 gcctgccgta taaagcgtgt tgccgcactg gcttaaaatc gccgccgtca tctctttaac
    29281 ggaggttttg ccggaggacc ccgtcagagc aaccacgcgc gccggaactt gctggcgaac
    29341 ccatgcagcc agttcaccaa acgccagacg cgtatccttg acgattaact gcggcaggtc
    29401 gatgtccagc ggacggctaa ccagtagtgc gcctgcgccg ccagctttcg cctggtcggc
    29461 aaaatcatga gcatcgaaac gttcgccttt cagggcaaca aacaggcagc ccggcgtcag
    29521 ttttcgggta tcagtggtta cagcatcaaa ggtgatatct gcaccttgca gttcaccgtt
    29581 gagaatgtcg gtaagttggc taagggttac gctaatcatg caatcacccc cagcagacgc
    29641 gccaccgtga cgcgatcgga gtagtccaga cgctgattgc caacaatctg gtaatcttca
    29701 tggcctttgc ccgcgaccag caccacatcg ttctctttag cctgcataac ggcgcaagtc
    29761 accgcttcag cacggccttc catcactttg gcatgtccgg catctaacat tcccgccaga
    29821 atatcgttga tgatggcacg cggttcttcg gtacgcgggt tatcgtccgt caccaccgcc
    29881 acgtcagcaa actcttcggc aattgcgccc atcagtggac gcttaccttt atcgcgatcg
    29941 ccaccacagc caaagacaca ccacagcttg cccgcacagt gcagacgcgc cgcctgtaag
    30001 gctttttcca gtgcatccgg tgtatgcgcg taatccacca ccaccgtcgg tttgcctggc
    30061 gcagtgaaca cttccatacg tccgcaaacc ggttgcagac gcgcggcggt tttcaacaga
    30121 tcagccaatg gatagccgag tgccaacagt gtcgccagcg cgagcagcag gttgctgacg
    30181 ttaaaagcgc ccatcagatg gctttcaatt tcgccatcgc cccaacttga gctaaagcga
    30241 atcgtcgcac cgctgtcgtg atagttcacg tcgatcgctt ttaaccagcg tccgtgacag
    30301 ttcggattga tatgatcttc cattgatacc gcaaccgcgt ccggcagttt tgccagccag
    30361 cggcggccca cttcatcgtc ggcgttaata atcgcctgac cgcaatgatg ttcagaataa
    30421 agcagccatt tcgcagcttc gtagtgttcc atatcaccat gataatcaag gtgatcgcgg
    30481 cttaagttgg taaagaccga cgccgcaaat ttcaatgccg ccacacggtg ctgtaccagc
    30541 ccgtgggagg aaacttccat tgcgcaaaaa gtcgcgccct gatccaccag ccccgccagc
    30601 tcatgctgaa catcgactgc cgaaccggta gtattttctg tcgggatcac tttccccagc
    30661 agaccgttac caacggtgcc cattaccgcg ctagtttcgc caagcagttg gctccactgc
    30721 gccagcagct gggtcgtcgt ggttttgccg ttggtgcccg ttacgcctac gagacgtaaa
    30781 ttgtcagagg gttcatggta aaagcggccc gccagtgcag ataaacgctc attgagctgg
    30841 ctgagataga tgaccggtac gccgtgcatt tcacggattt caccgtcggt cgcctcatct
    30901 ttcgcctctg caataatggc agccacacct tgcgctatcg cctgcgggat atatcgacgc
    30961 ccgtccgcct gatgacctac tacagctaca aagagatcgc ccgccgcagc cacacggctg
    31021 tcgagtgtca tctctcgcag tgctcgcgaa ggtgcgtctg gcacccacgg agcaagaagg
    31081 tcgcgcaaat tacgatctgc cacctgtccc ctcgccttga ttaatcacaa attcattttt
    31141 atcgcccgtt gtcagcgcat ccggctcgat gttcatggta cgcaatacgc cgcccatgat
    31201 ggcaccaaag accggcgcgg aaacggcgcc gccgtagtat ttacccgcct gcggatcgtt
    31261 gataacaaca accagcgcga agcgcggctg actcgcaggc gcaacgcctg cggtataagc
    31321 aatatattta ttgatgtagc gaccgtccgg cccgaccttt ttcgcggtac cggttttaat
    31381 ggcgatacga tagcctttaa tcgccgcctt cacgccgccg ccgcccggta gcgccacgct
    31441 ttccatcata tgcaccacgg tgcggacaat ggattccggg aagacacgtt caccgggaac
    31501 cggggggtca actttggtaa tcgacagtgg gcgataaatg ccgtagctgc cgatagttgc
    31561 gtagactcgc gctaactgta acggtgttac cattagcccg tagccgaaag agaaggtggc
    31621 cctctctatg tcagaccacc gttgtttttg aggatataag ccactgcgtt ctccgaccaa
    31681 ccccaaattg gtcgcttttc ccagtccaaa acgtgagtaa gtatctacta acgctgagga
    31741 cggcatcgct aacgccagct tggaaacacc gacgttactc gacttctgta ataccccggt
    31801 cagggttaat tcgctgtagc gtgccacatc tttgatttcg tggccgttaa ttcgataagg
    31861 aacggtattg agtaccgagt tttcccgcac cacgccacgt tgcaacgcag tcatcaccac
    31921 catcggttta accgttgagc ccggttcaaa cacgtcggtg atggtacggt tacgcatcgc
    31981 ctctttcggc gtgccactca gattgttagg gttgtatgat gggctgttag ccatcgccag
    32041 cacttcaccg gtgttgacat ccaccagcac ggcgctacca gattcagcct tgttaaaggc
    32101 caccgcattg ttcagttcgc gataaaccag cgcctgcagg cgttcatcaa tactcagcgc
    32161 caggttgtgc gcagcctggc tgtcagtaga agaaatatct tcaattacgc gaccatagcg
    32221 gtctttacgc acaatgcgct cacccggctg cccggtaagc catttatcga aactcttctc
    32281 gacgccctca atcccctggc tatcgacgtt agtaaagccg atgaggtgag cagtcacttc
    32341 gccggatgga taataacggc gagattcttc acgcagatga atccccggca gtttcagttt
    32401 tttgatgtag tccgccatgt cagggtttac ctgacgcgcc agataaataa agcgcccttt
    32461 cgggttggcg ttaatgcggg ctgaaagctg atccagcgga atattgagcg cgttagccag
    32521 cgccttccag cggtcaccga cgctgatacc gccagcgtca tgcacttctt tcgggtcagc
    32581 ccaaatcgct tttaccggca cgctcaccgc taacgggcga ccagaacggt cagtaatcat
    32641 gccgcgggag gtggaaactt gctgaacgcg aagagaacgc atgtcgccct ctttcaccag
    32701 catatccggg gagataactt gtaaccacgc tacgcgtccg agcagaaaag ccagcgccag
    32761 gagaatacag ccgcataaca acgcaaaacg ccaactgata aagttggcat gttcttcctg
    32821 acgttttggt ttctgcgttt tcgccgctgc tttcatgcgt cgcgtttatc cttatttttg
    32881 cactacgata ttttcttgtg acggatcaac atgctgcatt tgcagctttt ccgtggcgat
    32941 cctttccacc cggctatggt cgccgagcgc attctcttca aggatcaggt tgcgccattc
    33001 aatgtctaaa gcatctcgct ccagcaccag ttgttcgcgc tgagcggtca gtaaacgggt
    33061 atggtgcgcc gtggttacca cggtcaccgc cgtcaaaata atgcaaatga acaggcagag
    33121 tggcagcttc ccaaatcgca aaagatcgtc accgataaca ccaggcaatg catggcgctc
    33181 gtggcttccc atcgatcctt taactttgct tagagcttct gtcactctgc tgatcatgcg
    33241 ttcgtcctct ctgcaatacg cagaactgaa ctacgggcac gaggattctc agccacctct
    33301 tcttcgcccg gcattaactt gcctagtgct cgcagctgac ggccacccag ttttttgagc
    33361 tgctcttcag tcatcggtaa ccctgccgga acttgcggac cgcggctgtt ttcacgcata
    33421 aaacgtttca caatacggtc ttccagcgag tggaagctga tgatcgaaag ccgcccaccc
    33481 ggggccagca cgttgagcga gctttttagc gcctgctcta tctcctccag ttcactgttt
    33541 acccaaatgc gcaccgcctg gaaggtacgg gtcgcgggat gtttaaactt atctttcacc
    33601 ggcgttgcag cagccacgac ttccgccagt tctttggtgc gggtcatcgg ctgttcgcgg
    33661 ttacgctcga caatggcgcg ggcaatgcgt ttggcaaaac gctcttcacc ataggttttc
    33721 aatacccagg cgatatcggc ttcttctgcg gtttgtagcc attcagcggc tgactgccca
    33781 cgggttgggt ccatacgcat gtccagcgga ccatcgcgca taaaggaaaa gccgcgctca
    33841 gcatcatcaa gttgcggtga agagacgcca agatcgagga gaatgccgtc gatcttgccg
    33901 ataagatcgc gctcggcaac gtattcgccc agcgcggaaa aaggtccgtg gatgatggag
    33961 aagcgtggat catcaatagt cttcgcaacg gcgatagcct gcgggtcgcg atcgatcgcc
    34021 agcaaacgcc cctcttcgcc aagctgcgag aggatcagac gtgagtgacc accgcgacca
    34081 aaagtcccat caatgtagat gccatcagga cggatattga ggccattaac ggcttcatcc
    34141 aacagcaccg tagtatgttt atagttttcc atcattttat agagacaagt cctgcagtcg
    34201 ctccgataag tctccggtag ccaactgctc tgcgtcgata tcttccttga cctgttgatg
    34261 ccaggttgtt tcatcccaca gctcaaactt gttgaactgt ccaaccagca tcacttcttt
    34321 tgtcagcccg gcatgttgcc gcagtactgg cgcgattaac aatcgacctg cgccatccat
    34381 ctgacattcg ctggcatgac ctaacagtag gcgctgcaca cggcgctcaa ccgggttcat
    34441 gctcgacaga cgcgataatt tttgctcgat aatttcccat tcaggcaggg ggtaaagcag
    34501 caggcacggg tgatgaatgt caatggtgca aaccatttga ccggcagcgt tctcaagcag
    34561 ctgttcccga taacgggtag gcactgataa gcgccctttg ctgtcgagat tgactaacgt
    34621 tgctccccgg aacatgccag cctcacccct tattcccact ttgccccaca atttcccact
    34681 gaaaggagtt tacggagcgg aggaaaagct tgtcaagcca gtcacaaggc ataccgaggt
    34741 aaaaaacccg atgtttacgg ctaatatcag cgttgtttat tttacgtcca acgtgcgaag
    34801 caaattaacg ttatgaatag ctgtaagaaa aaaaacctca tcgtcatcac cgttttaaaa
    34861 tgactcacta ttttccacag caaatataaa gtgtcagttt gcgacgcgag cggcatttta
    34921 ggacatatct tccccggtta acagtccctg ttgcgtctgt gtagcgcttg atgccagcgg
    34981 gtttgcgcaa aagcaaagaa aagtgcttgt taaatcggca attcatctct gcatgtaaca
    35041 aaataatgca aaaacgcgct gtcgatatca cttaattatt gtgaatttca gctggtgagt
    35101 taattaacgg gcgggtttaa aagcatttat caataatagt caggaatagt cttatttact
    35161 ttaaggatat tgatgtccag tcccgtactc tacgcgccag agtgaaattc acctggcgcg
    35221 tatttttgtt cgcagcttag ctacggctga gcacaccgcg gcgatagaga ttacgtttaa
    35281 tgcgcgttaa accaggtttt ggcttacgcg gttcgtccag gcttgccagg acaatctcca
    35341 gcacacgctc tgcgacatcg cggtgacgtt gagccactgc cagcaccggg cactgtaaga
    35401 agtcgagcag ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttaccat
    35461 cgcgacgtag cgtgacatcc atcacccctt gcaacaacgc aaacgacgtt gtgaacagcg
    35521 cctgcggcat cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct
    35581 catagctgtt ggcatacagg aaatgcactt cgcgcggatc atctttccag gcagtacgga
    35641 aaccttgttc acgcaggaag ctgacagaaa gctccggcag cgcgccaaga taaagcaccg
    35701 tctcggcggg aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac
    35761 caaccacgct ggtaaagtgt tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg
    35821 ggtcgttagc ccagcgttga taaaaaggat gctcaggagg caacgacgtc gaaacaataa
    35881 tggcatcgac ctgacgctgt aaaaggtgct caatacagcg catttcgttg tctggctgat
    35941 cttctgagca ggcaatcagc agttgataac cccgttgccg cgcctggcgt tcaagatagt
    36001 tagcgatgcg ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacgtg
    36061 tgcgtccagc acgaagccca gctgccacgg cgttcgggtg gtaattgtgc tcacgcacga
    36121 cagccatgac tttttcaacg gttttgtcgc tcacacggta ttgcttcgct ttgccgttaa
    36181 taacatagct tgcagtggtc cgcgacactc ccgccagccg agcgatttca tccagtttca
    36241 caattgcccc ttgcgtaaaa tgtaaaaacc atagccattg tactggtatg ggttaaattc
    36301 attaacatct aagcgcagaa aattaactgc ggcaactgct tttattccag gttcccactg
    36361 atttcgcaaa aaaagcccaa cgtgacatgt tgggctgtaa attgcgcatt gagatcattc
    36421 tcaacgcatt attttatcgc cgcgcgaaag tccgaccaca ccagagcgag caacttccac
    36481 aattttcgcc acatcgcgaa tcgatgctaa aaatgcatca agcttatcgc tggtgcctgc
    36541 taattgaacg gtataaagcg agggtgtgac atcgataatt tgcccacgga atatttccgt
    36601 attacgtttc acttcgtcac gcccgtaacc gctggcctga attttcacca gcatgatttc
    36661 ccgctcaaca tgcgcgccct gccccaactc actcacgcgc aagacatcga ccagcttgtg
    36721 taattgcttt tcgatctgct caagtacttt ttcatcgccc acggtctgga tggtcatacg
    36781 cgataatgtc ggatcgtcgg ttggcgcaac ggtcaggctt tcaatgttgt agccacgctg
    36841 ggaaaaaagg ccaatcacgc gggataacgc gcctgattca ttttcgagta agactgataa
    36901 tatccggcgc ataatcaggt tctctccgtt ttgcttaacc acatttcatc cattccgccc
    36961 ccgcgaatct gcatcgggta gacgtgctcg ctgccatcga cggtaacatc aacaaacacc
    37021 aggcgattat tgcgcacctg ttccagcgcc tcgctaagtt tgctttccag ctcatgcgga
    37081 tgagaaatct ggatcccgac atgcccatag gcttccgcca gacggacgaa atcgggtagt
    37141 gattgcatat aagattgtga atgacggccg gaatagatca tgtcctgcca ctgcttcacc
    37201 atccccagat agcggttatt gagattcacc accagtacgg gcaactcgta ttgcaacgcg
    37261 gtagacagtt cctggatgtt catctgaata ctgccgtcgc cagtgacgca aaccacggtt
    37321 tcttctggca acgccatttt gacgcccagt gccgcaggta aaccaaaacc catcgtgccg
    37381 aggccaccgg aattgatcca gcgacgcggt ttgtcgaatg gataataaag tgcagcaaac
    37441 atctggtgct gcccgacatc ggacgtcacg taagcgtctc ccttcgtcaa ccgccaaaga
    37501 gtctcgatca ccgcctgcgg tttaatcttt tcactgtgag tgtcatattt caggcactga
    37561 cgagcgcgcc actgttcaat ttgctgccac cagtcgcgga tctcatccag tggttgatgg
    37621 gcggattctt gcgacaagag ttcaagcatt tgttcgagga cctggcgagc atcccccaca
    37681 atcgggatat ccgcagtcac ggttttagaa atggaagtag gatcaatatc gatatgcaga
    37741 acagtggcat ttgggcagta ctttgccaga ttgttcgtcg ttcggtcatc aaatcgtacc
    37801 ccgacggcga aaatcacatc cgcgttatgc atcgtcatat tggcttcgta ggtaccgtgc
    37861 attcccagca tgcccagtgc ctgacgatgc gttgccggaa acgcccccag ccccatcaat
    37921 gaggaaacaa cgggcagatt caacgcctcc accgtttctt tcaactgctg atggcagccc
    37981 gccgtgattg ccccaccgcc tacgtagaca accggttttt ttgccgctac cagcgtttgc
    38041 agagcacgct taatttgccc tttatgtccg gtagtagtgg gattgtaaga acgcatactg
    38101 accgactccg gccagacata gggtaatttg ttcgccggat taagaatatc tttcggtaaa
    38161 tcaacgacca ctggtcctgg gcgaccactt gccgccagcc agaaagcctt tttcagcacc
    38221 tgcggaatgt cttccgtttg cttaaccaga aaactgtgtt taaccaccgg tcgcgaaatc
    38281 cccaccatgt cgcactcctg aaaggcatcg taacctatca acgaggtcgc tacctgcccg
    38341 gaaaggacaa ctaatggaat ggaatccata taagcggtgg cgatgccagt aatcgcattg
    38401 gtcgcccctg gacccgacgt taccagcacg acgccgactt ccccggtcgc gcgcgccaga
    38461 ccatcggcca tatgcactgc cgcctgctca tgacgaacta aaacatgatc gataccaccc
    38521 acggtatgca atgcatcgta aatatcaagg actgcgcctc cgggataacc gaatacttgt
    38581 ttaacgccct gatcgataag cgatcggacg accatctcgg ctccagacaa catctccatg
    38641 gcctgcctca ctgtttgacg gaaaaaatgt gtaaaacaca ttaaccgtcc gatcttgctt
    38701 aagcaattgg cagccagaga cataaacgcc gggtaataag aataaacagg aggaaaggtg
    38761 aaaagaaacg ataaaatcct ccattacgaa ggataaaaat gataataaat cggctaaatc
    38821 ccaccactta cagcaatttc tctatttttt taattaatcc taaaataagg agcccacata
    38881 ttcagaataa aattctacat tcaataaaaa atgcagaata aaccagacat tcatgtctga
    38941 cctattctgc attcagttag cgtttgcaaa ttgagactaa ttgctcttcc atccactgat
    39001 ggcctttatc gcgcccggca gcttcatgcc aggagagata acaggttctg ctatttagtt
    39061 ttaacggcag cggtaatacc tgtaattcta aggattcagc gaactcttca gccagccaac
    39121 gcggcgcaat agcgaccaaa tgcgtttgcg acaccacgct aagtacgctc atcattgcca
    39181 tgccctgata cgcgatactg gcttgcttat ctaccgtgtc ataccaaggt tgactaaatg
    39241 acgcgaaacg atcgagcgaa accgccgcat gttgttcgtt ataaacatca tgtttcagta
    39301 acgggccttt aattgttgga tgatttttgc tggctaccag caccatttca tctttaaata
    39361 atggcacgct ggtaaattca ggacgatgga agtcttcata gctaatcaca aactccgttt
    39421 cctgataacg cagctgatgt tcagtgttct gatttaatga agacttgaac ataacatgta
    39481 tatttggcgc aatctgctca atgtgattat aaatctgcga ggtcagaata ctgtctaacg
    39541 ggctgcaaac acaaagatga aatacacgtt cactgctcgc gggttcaaaa cctgaaccag
    39601 gcaattcatt ttgtactagt tgcaatgcct gacgaactga accaaaaagt tgaaatgcgc
    39661 gagcagtcgg ttgaatacca cggccataac gaacaaaaag ctcgtcatta aacatcacct
    39721 tcaggcgtgc aacagcgtta ctgaccgcag gttgcgacat tcccagaaca tgagcagcac
    39781 gggtaatgtt ttgctcctgc atcacggcat cgaaaacggt taataagttg agatcgacca
    39841 tgcgtagctg tggcttgctg aactccgccg tctctggatg atctgtttgt acctctggca
    39901 tacttaactc cactgtcaca cttaactccc tttcccttat tggaatgcag agaaataatc
    39961 ctcgagaata
//
LOCUS       DAPGRN010000032        39314 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPGRN010000032 DAPGRN010000000
VERSION     DAPGRN010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.032"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            179..1834
                     /gene="aslA"
                     /locus_tag="RHW33_001823"
     CDS             179..1834
                     /gene="aslA"
                     /locus_tag="RHW33_001823"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HDV1645333.1"
                     /translation="MEFLFSPKRLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIKQLPFSKDDVHAVRGGEQQAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYGDCMVEMNDVFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(2002..3237)
                     /locus_tag="RHW33_001824"
     CDS             complement(2002..3237)
                     /locus_tag="RHW33_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV1645334.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRYYIAASEPQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAAREGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMVDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(3384..3460)
                     /locus_tag="RHW33_001825"
     tRNA            complement(3384..3460)
                     /locus_tag="RHW33_001825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3424..3426),aa:Pro,seq:tgg)
     gene            complement(3503..3589)
                     /locus_tag="RHW33_001826"
     tRNA            complement(3503..3589)
                     /locus_tag="RHW33_001826"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3553..3555),aa:Leu,seq:cag)
     gene            complement(3610..3685)
                     /locus_tag="RHW33_001827"
     tRNA            complement(3610..3685)
                     /locus_tag="RHW33_001827"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3650..3652),aa:His,seq:gtg)
     gene            complement(3744..3820)
                     /locus_tag="RHW33_001828"
     tRNA            complement(3744..3820)
                     /locus_tag="RHW33_001828"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3784..3786),aa:Arg,seq:ccg)
     gene            complement(3923..5308)
                     /locus_tag="RHW33_001829"
     CDS             complement(3923..5308)
                     /locus_tag="RHW33_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1645335.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5499..6239)
                     /gene="rffM"
                     /locus_tag="RHW33_001830"
     CDS             complement(5499..6239)
                     /gene="rffM"
                     /locus_tag="RHW33_001830"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV1645336.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6242..7594)
                     /gene="wzyE"
                     /locus_tag="RHW33_001831"
     CDS             complement(6242..7594)
                     /gene="wzyE"
                     /locus_tag="RHW33_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV1645337.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7591..8670)
                     /gene="rffT"
                     /locus_tag="RHW33_001832"
                     /gene_synonym="wecF"
     CDS             complement(7591..8670)
                     /gene="rffT"
                     /locus_tag="RHW33_001832"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV1645338.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKSR
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMEPSLNTMANDRQREGKMTVLVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8667..9917)
                     /gene="wzxE"
                     /locus_tag="RHW33_001833"
     CDS             complement(8667..9917)
                     /gene="wzxE"
                     /locus_tag="RHW33_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV1645339.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     IAYYVSYRFGGYEGALLGLALIPALVVMPAAVMLIKRGAIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9919..11049)
                     /gene="rffA"
                     /locus_tag="RHW33_001834"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9919..11049)
                     /gene="rffA"
                     /locus_tag="RHW33_001834"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV1645340.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(11054..11728)
                     /gene="rffC"
                     /locus_tag="RHW33_001835"
                     /gene_synonym="wecD"
     CDS             complement(11054..11728)
                     /gene="rffC"
                     /locus_tag="RHW33_001835"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV1645341.1"
                     /translation="MPVRASIEPLTWENDFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11706..12587)
                     /gene="rfbA"
                     /locus_tag="RHW33_001836"
     CDS             complement(11706..12587)
                     /gene="rfbA"
                     /locus_tag="RHW33_001836"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV1645342.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12606..13673)
                     /gene="rffG"
                     /locus_tag="RHW33_001837"
     CDS             complement(12606..13673)
                     /gene="rffG"
                     /locus_tag="RHW33_001837"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV1645343.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKSLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIAHELGWLPQETFESRMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(13673..14935)
                     /gene="wecC"
                     /locus_tag="RHW33_001838"
     CDS             complement(13673..14935)
                     /gene="wecC"
                     /locus_tag="RHW33_001838"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV1645344.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14932..16062)
                     /gene="wecB"
                     /locus_tag="RHW33_001839"
     CDS             complement(14932..16062)
                     /gene="wecB"
                     /locus_tag="RHW33_001839"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV1645345.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(16118..17164)
                     /gene="wzzE"
                     /locus_tag="RHW33_001840"
     CDS             complement(16118..17164)
                     /gene="wzzE"
                     /locus_tag="RHW33_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV1645346.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(17176..18279)
                     /gene="wecA"
                     /locus_tag="RHW33_001841"
     CDS             complement(17176..18279)
                     /gene="wecA"
                     /locus_tag="RHW33_001841"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV1645347.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18519..19778)
                     /gene="rho"
                     /locus_tag="RHW33_001842"
     CDS             complement(18519..19778)
                     /gene="rho"
                     /locus_tag="RHW33_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV1645348.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19863..19964)
                     /gene="rhoL"
                     /locus_tag="RHW33_001843"
     CDS             complement(19863..19964)
                     /gene="rhoL"
                     /locus_tag="RHW33_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV1645349.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(20105..20434)
                     /gene="trxA"
                     /locus_tag="RHW33_001844"
     CDS             complement(20105..20434)
                     /gene="trxA"
                     /locus_tag="RHW33_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV1645350.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20565..21830
                     /gene="rhlB"
                     /locus_tag="RHW33_001845"
     CDS             20565..21830
                     /gene="rhlB"
                     /locus_tag="RHW33_001845"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV1645351.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21966..23450
                     /gene="gppA"
                     /locus_tag="RHW33_001846"
     CDS             21966..23450
                     /gene="gppA"
                     /locus_tag="RHW33_001846"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV1645352.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENTLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(23497..25518)
                     /gene="rep"
                     /locus_tag="RHW33_001847"
     CDS             complement(23497..25518)
                     /gene="rep"
                     /locus_tag="RHW33_001847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV1645353.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAERDPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            25735..26184
                     /locus_tag="RHW33_001848"
     CDS             25735..26184
                     /locus_tag="RHW33_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV1645354.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGPFSTTYYDGRIPPEMIKN
                     RLLVVLNGKINGNACIVVPLSTTRDHDKLKRGMHVEIARNVINDLQFFDQQIRWAKTD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            26383..26664
                     /gene="ppiC"
                     /locus_tag="RHW33_001849"
     CDS             26383..26664
                     /gene="ppiC"
                     /locus_tag="RHW33_001849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV1645355.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(26710..28185)
                     /gene="ilvC"
                     /locus_tag="RHW33_001850"
     CDS             complement(26710..28185)
                     /gene="ilvC"
                     /locus_tag="RHW33_001850"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV1645356.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            28335..29228
                     /gene="ilvY"
                     /locus_tag="RHW33_001851"
     CDS             28335..29228
                     /gene="ilvY"
                     /locus_tag="RHW33_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV1645357.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(29280..30824)
                     /gene="ilvA"
                     /locus_tag="RHW33_001852"
     CDS             complement(29280..30824)
                     /gene="ilvA"
                     /locus_tag="RHW33_001852"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV1645358.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(30827..32677)
                     /gene="ilvD"
                     /locus_tag="RHW33_001853"
     CDS             complement(30827..32677)
                     /gene="ilvD"
                     /locus_tag="RHW33_001853"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV1645359.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHSDRKQLFLNAGKRIVELTKRYYEQNDDSALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(32742..33671)
                     /gene="ilvE"
                     /locus_tag="RHW33_001854"
     CDS             complement(32742..33671)
                     /gene="ilvE"
                     /locus_tag="RHW33_001854"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV1645360.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKEQGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(33691..33954)
                     /gene="ilvM"
                     /locus_tag="RHW33_001855"
     CDS             complement(33691..33954)
                     /gene="ilvM"
                     /locus_tag="RHW33_001855"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV1645361.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(33951..35597)
                     /locus_tag="RHW33_001856"
     CDS             complement(33951..35597)
                     /locus_tag="RHW33_001856"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV1645362.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(35600..35650)
                     /locus_tag="RHW33_001857"
     CDS             complement(35600..35650)
                     /locus_tag="RHW33_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645363.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(35737..35835)
                     /gene="ilvL"
                     /locus_tag="RHW33_001858"
     CDS             complement(35737..35835)
                     /gene="ilvL"
                     /locus_tag="RHW33_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV1645364.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            36188..37708
                     /locus_tag="RHW33_001859"
     CDS             36188..37708
                     /locus_tag="RHW33_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV1645365.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(37733..38071)
                     /gene="maoP"
                     /locus_tag="RHW33_001860"
     CDS             complement(37733..38071)
                     /gene="maoP"
                     /locus_tag="RHW33_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV1645366.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            38190..39029
                     /gene="hdfR"
                     /locus_tag="RHW33_001861"
     CDS             38190..39029
                     /gene="hdfR"
                     /locus_tag="RHW33_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV1645367.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(39125..39200)
                     /locus_tag="RHW33_001862"
     tRNA            complement(39125..39200)
                     /locus_tag="RHW33_001862"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39165..39167),aa:Trp,seq:cca)
     gene            complement(39209..39285)
                     /locus_tag="RHW33_001863"
     tRNA            complement(39209..39285)
                     /locus_tag="RHW33_001863"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39249..39251),aa:Asp,seq:gtc)
BASE COUNT         9639 a        10718 c         9893 g         9064 t
ORIGIN      
        1 actgcgccag aggtaggatt gaaaacgctc tcctgatttt ccaattcatt ttctggataa
       61 ataaataatt tatttttgtc actattattt atgtaatcat cctgtcaggg agagggatct
      121 caattatcaa tgcttaatta cgtcatcatt ttgatgacat gaaaccggag cattttgcat
      181 ggaatttttg ttttcaccca aacgtcttgt tgttgctgtc gccgccgctc ttcctctcat
      241 ggccagcgca gcagataccc cgtcaactgc caccgcacgc aaaggctttg ccggatacga
      301 tcacccaaac cagtatctgg ttaaaccggc gaccactatt gccgacaata tgatgccagt
      361 aatgcagcat ccggcgcagg ataaagaaac ccagcagaag ctggcagaac ttgagaaaaa
      421 aaccggtaag aaaccgaatg tggttgtttt cttgctggac gatgtgggct ggatggacgt
      481 cggttttaac ggtggcggcg tggcggtggg taaccctaca ccagatatcg acgccgttgc
      541 cagccagggg ctgattttaa cttcggcgta ttctcaacca agctcttccc caacccgcgc
      601 caccattctc accggacaat actccatcca ccacggcatt ctgatgccgc caatgtacgg
      661 gcaaccgggc gggctgcaag ggttaaccac gctgccgcag ttgctgcacg atcagggcta
      721 cgtcactcag gccatcggga aatggcatat gggggaaaac aaagagtcgc agccgcagaa
      781 cgttggcttt gatgatttcc gtggctttaa ctcggtgtct gatatgtaca ccgaatggcg
      841 cgatgttcac gtaaacccgg aagtggccct gagtccggac cgttctgaat acatcaagca
      901 attaccattt agcaaagatg acgttcatgc ggtgcgcggc ggcgaacaac aggccattgc
      961 cgacattacg ccgaaatata tggaagatct ggatcaacgc tggatggaat atggcgttaa
     1021 gttcctcgac aagatggcga agagtgataa gcctttcttc ctctattacg gcactcgcgg
     1081 ctgtcacttc gataactacc cgaacgccaa atatgcgggt agctctccgg cacgcacctc
     1141 gtatggcgac tgcatggtgg aaatgaacga tgtgttcgct aatctgtata aagcactgga
     1201 gaaaaacggt cagcttgata acacgctgat tgtctttacc tccgataacg gaccggaagc
     1261 cgaagtaccg ccgcacggac gcacaccgtt ccgtggtgcg aaaggatcta cctgggaagg
     1321 cggcgttcgc gttccgactt tcgtgtactg gaaaggcatg atccaaccgc gtaaatctga
     1381 cggcattgtt gatctggcag atctcttccc gaccgcgctg gatctggcag ggcatcctgg
     1441 cgcgaaagtg gcgaatttag tgccgaaaac cacctttatc gatggtgtgg accagacatc
     1501 tttcttcctg ggaacaaatg gtcagtctaa ccgtaaggcc gagcactact tcctcaatgg
     1561 taaactttct gctgtgcgta tggatgagtt caagtatcac gtcctgattc agcaacctta
     1621 cgcttatacc cagagcgggt atcagggtgg attcaccggc acagtaatgc aaacggcggg
     1681 atcgtcggtg tttaacctct acaccgatcc gcaggaaagc gactccatcg gcgtgcgcca
     1741 tattccgatg ggtgtaccgc tacagaccga aatgcacgcg tatatggaga tcctgaaaaa
     1801 gtatccacca cgcgcgcaga ttaaatctga ttaagccggc gatatcgctg agatctgcct
     1861 ttgccggatg cgatgctgac gcatcttatc cagcctacag aacgctgcaa tttattgaat
     1921 ttgcacgatc atgtaggccg gataaggcgt ttacgccgca tccggcaatc aaccgcaggc
     1981 ggccacaaaa tggccgcttt tttacttact caccaccagc aaatgcgcat gcataatgtc
     2041 gctggccgga cgaccgtgcg ccagcaaatc agccatagct ttaagatatg gcggtagatg
     2101 gcggaaataa cgctgatatc cggcacacaa ataattcagc cccggtttac cgctagcatc
     2161 gagcatgaag cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt tgcaactgcg
     2221 acactgcgcc ggtaactgct taaatttatc ttcaccaaaa gcctgctgct gcggagaatc
     2281 taccatttct gcaattgttt gctggtgcat attccccaac cgatattgcg gataaacata
     2341 gtgatcgcag gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg
     2401 ctgatgatgg caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc attcgatatt
     2461 catcacaaaa atcttgccga catcacgttt gatccagtgg tcgaaaatcg ccaccagaaa
     2521 ttcaccaaat tcatcggggc gcaccgacca ttccgttagc tcaccctgaa tatcaccagg
     2581 cgcatgcaat ttcagtcctt cgcgggcagc cgtttcatca gccaagcgct cgaccaccgg
     2641 aataaactgg atgaattcca ctcccgcatc gcacaaaaaa tcatatacct gcaacggttg
     2701 ctgcgcgctg gtgcgattaa cgcagaccag cacgttatag tcgacatgat gtttttgcag
     2761 cagcgtcagg gcacgcatca ccagcttatg cgttggtctg ccacctttgg tcacgcgata
     2821 ttgattgtgg atctcagccg gaccatccag cgataaccca acaagaaaat gattttctgc
     2881 cagaaatgca caccattcgt catcgagcag cacgccgtta gtctggaagc tgttacttat
     2941 cttcctgcca gcaccatatt tcgcctgtag cgctacggca cggcggtaaa actccagccc
     3001 gagtagcgtt ggttcgccgc cctgccaggt aaaagccact tcgttttgcg gttcgctggc
     3061 agcgatatag taacggacat acgcttccag cgtgtcatcg tccatatgcg tgactggttt
     3121 ttcgcggtaa agggattgtt tttcgagata aaaacagtag tcacagttca gattacaatc
     3181 ggaaccgctc ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt
     3241 cgctccttgc ctgaagtcat ccttcaatac gttaagggcg aaatcatacc gtggggcaat
     3301 gagttattac gggcgtttat tgcgaaggaa gggtgataga atgcacagaa aacaaaaaac
     3361 cccgccgaag cggggttcaa aattggtcgg cgagagagga ttcgaacctc cgacccactg
     3421 gtcccaaacc agttgcgcta ccaagctgcg ctactcgccg aaatactgct ttttgaattt
     3481 ttagttcaat tctttaaagt cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa
     3541 ggacactaac acctgaagct agcgcgtcta ccaattccgc caccttcgca ttgtcacaac
     3601 ttctaataat ggggtggcta atgggattcg aacccacgac aactggaatc acaatccagg
     3661 gctctaccaa ctgagctata gccaccacta caaatcttgt tacgcggtat tactaccacc
     3721 gcagctcaag cgccgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc
     3781 ctccggaggg cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggtg
     3841 gataatacgg acttcccacc cctctgtcta gtcccttttt aaataaattg cgcgtttggt
     3901 tacggtttgt gcgttttgct gcttattcct ccagtttatg cgctttcccg tggcgattaa
     3961 ggccaaaaac tttataaatc gccgtcaccg ccagcataaa gatgatgcca acaaacagcg
     4021 acatacgcgt atcttcatta aagtacatgc cgatcaaaac gcaaatcagg aatgccattg
     4081 ttacgtagtt ggcccacggg aacaggattg agcggaacgg atggctggca atcgccgctt
     4141 tatgtgcacg ccgaaaacgc agctggctta tcaatatcac aaaccatggc accatccccg
     4201 gaagcacgct ggcactgtag acgtagacaa acacacgctg cggattggga atgatgtagt
     4261 tcaggcatga gccaattagc agaatagcaa tagatactgc cacacccgca accggtacgc
     4321 cgtgacggga aactttcgcc atcgccgccg gtaactgacg gtttttcgcc agtgcgtaga
     4381 gcatacgtcc gcaactgtac atgccgctgt tacagccaga gagcgcagcc gtcagcacca
     4441 caaagttgat aatgcccgcc gctgcggtaa taccgatttt ggcaaaagtc agtacgaacg
     4501 ggctgccgtt gctgcctatt tcattccacg ggaagatggt gacgataacg aaaatcgcgc
     4561 ctacgtagaa aatcaggatc cgccacagca ccttgcctac ggcactgcgc agcgtcacct
     4621 gcggattctt cgcttcaccg gcagtaatgc caatcagctc cacgccctgg taggacgcca
     4681 ccacaataca cagcgcggtc aggaaccctt tccagccacc cgcaaagaaa ccgccatgct
     4741 ctgtgagatt gctaaaacca atcgactgcc cgccattgcc aaagccaaag aaaatcacgc
     4801 ccaggccaat gacaatcatc acgataatcg tggtgacttt gatcatcgcg aaccagaact
     4861 cgatttcgcc gtacaaccgc accgccgcca gattcgccaa cgccaccagc gccaccgcga
     4921 tcaatgcggg tatccactgc gccatctccg ggaaccagaa ctggacataa acgccaatgg
     4981 cggtgatttc tgagatcccc acggccatcc acataaacca gtaagaccag gcggtgagat
     5041 agccaaaaaa cgggctcata taacgatgcg cataaacggc gaacgaaccg gtaaccggtt
     5101 cgaggaacaa catttcgccc attgaacgca tgatgaaaaa gacgaatagc ccggcgatga
     5161 tataggccaa caatacggat ggcccggccc atttcagggt actggcggcc cccataaaca
     5221 agccgacgcc aatggtgccc cccagggcga tgagttcgat atgtcgagct tccagcccac
     5281 gctgtagctc tggtttgtta tctgccataa atcctcgtgt tgtgtttgca tgctttccgg
     5341 tgttaccggt tatcgttatg ggtacatcga gtgttgcgaa tgttttcgta attcaggaga
     5401 aatggcaaat aaagcattaa aaatttgaat gctttgtgta ataaaaaagc agacaggcga
     5461 cggagtgacc actccgtcgc tttacaaaga gaggaaaatc agaggttgcc ggtgtagtgc
     5521 cagcgtaaat aacgcagcaa acgaagctga cgcttaatgc ggctcggctg cgaaagcagg
     5581 cggtagagcc actccagccc cagcgtttgc cagattttcg gtgcgcgttt tacgtgaccg
     5641 gtgaaaacat cgtaagtccc gccaacgccc atatacagcg catctggatg caccagacgg
     5701 cagtcgcgca tgaagatctc ctgctttggc gatcccatcg caacggtgac gatttgcgct
     5761 ccgctggcat gaatgcgttc aaacagcgcc tgacgctgct cgggtttaaa ataaccatcc
     5821 tgactgccaa cgatattcac attccattgg ttgcgcagtt tagcttcagt ttgcgccagc
     5881 acttcaggtt taccgcccac aagaaatacc ggcgtccctt ctttgcctgc gcgcgccatc
     5941 agctcttccc agagatcggc accggcaacg cgggaaacct gcgcctgcgg gtacttttta
     6001 cgtactgaac gtacaacgct gatgccatcc gcatatttaa attcggcagc gttaattaac
     6061 tccctgacct cggcgttatc ttcaatggtc agcatttttt cagcattaat ggcaaccagc
     6121 gttccctgct taagctgccc gtcagcaaac agataatcga gggcatgctg catatcacgc
     6181 caaccaatca actgtaagcc acgcagcgta taggtcggtg ccgtggtgtt gttattcatt
     6241 gttatccttc aacctgcgtc cggagcgatg attttgtacg tttatgaatg agtccggcgc
     6301 tttcaaaaag ccagtacaac agttttgcga tcatcagaca tgcgccgaag accacgataa
     6361 aaaagactac gcgtgagaca aacgaatcca gcccttcacg cgccagcacg atcatattga
     6421 agatcgcccc aaagcagaaa ctgtgcaata tcgcagcctt atagcgatta gtctcgcggt
     6481 tgcccagctc atacagccag tcgaaccatt tgatgatcag tccaaccaca atcgccccga
     6541 gcgggatgaa caacgcgccg cccatcacca ccagtgagcc tataagcgta ggcgagatcg
     6601 ccagtccgga gtggttattc agcacttccc aggtaaagta gttggctgag ttcagcacca
     6661 tactcgggcg acccggccac agccaggaag ggataaagac atagaaatcg cggacaattg
     6721 gagccaggcc ctggaagtcg atgttgtcgt agttctgcaa cagcaacgcc agattctccc
     6781 acggcgagaa ggtgtcgcga gtgagataga gaaacgtata gaacgcttca tcgccgctca
     6841 cattcattcc atagcgtttt agcgccagcc agaacatgcc aacaatcccc agcacgcccg
     6901 ccgccgccag catccacaac gaaatccagc cgcgaataat gccaataaac aggaagatag
     6961 cgaatgcgat gatgatattg gcgcgagtgc cgccgacaat catataagtc agcaagccaa
     7021 aggcgaccgt gctgacgagg aaaaacagcc acgctttgct gtcctggcgc agaaagtaga
     7081 ccaccagcat cgccgggatg aaaaagtaaa agaagcgttt taacgccacg ccggagactt
     7141 cactggaaaa gatctgactg taggagttaa gccggaacag caaaaagccg ttgtgcatga
     7201 agaagatgcc gacgcttacc agcgcgatac ccatcaggat cacccacgta agattggtct
     7261 ccacgcggtt catggtaaac agcggacggc gcggtacatc agcaacgcgt ttgcgtaggc
     7321 gggttttgta ggtgacatag taaaccgcgt agaagcagcc cgcagaaagc aacgcctgca
     7381 acaagatttc tggcggcgcg acaccaacat caaagcgaaa taccagcacg ctggtcagcg
     7441 ggaagccgaa gaaaaaggtg agcaaaaaca gcaatgaaaa gaagacattg aagttaaagc
     7501 gcacacggcg aaactcaaac caggtcagcg tggcaataaa cagcgtgcag agcagccaga
     7561 ccacaaacag gccactgaat tgcagcagac tcatgcgacc tccccggcgg caatcgccaa
     7621 cgcccgctgc cagccttgta gatagttagg gctaaagaag gcaatggtgt ttttatccac
     7681 cgacgccaac tggcgctgcg cttcacgcac aatatcctcg ttgagatcgt cggtagtaaa
     7741 cagcactggc aaatgctgtt ccgtcatatc ctgccagaac ggattttccc ggttaagcac
     7801 acaaggaatg cccgcctgaa tcagtaagca cagcgtacca atgccctgct ggcgggcaaa
     7861 aataaagtaa ccgagatcgc actgacgaag tagcgccaga taggcgtcaa attccagttt
     7921 ttcgctcaga acttgcagat tttcttcgct gaataactcc agccccgcct gacgaacttc
     7981 ctcaatgtac gcttcgttat taggcggata tcccatcggc accaccactt ttaccgtatc
     8041 gccaaattgc tgatgaacgg cacgtaaggc ggcgacatgc tcattgctgc ggtcaccgga
     8101 gttccccacc agaacggtca ttttcccttc acgttgccga tcgttcgcca tcgtattgag
     8161 agaaggctcc atccgcgtcg ggaagtacag cagttcgccc cgcacctttg ggtgcgtttt
     8221 ggcaaaaaag ctcaaatcac cgcgggtggc aaatacacag ccgactcgct tttgcgccag
     8281 gcgacgtagc gggtaaaaaa gcttatatct caagccactg gaaagctcat acagatctgc
     8341 cccccagata tgccagaaaa actggcggga cttaatgcca ccactcagca gagccagcca
     8401 cagtttggga ttgaactgac cgtggaaaaa aaatcgctgc tgacggttag cttttgcttt
     8461 cgcgatgacc gcttccgcca gcgatttttt cccagggaaa aattgcacag aaagcgccgg
     8521 acagctatca cttaagccgt cgtccttgcc aacaaccata aactcgcgcg cgtgctcgct
     8581 cgtcgcggcc agcgcatcat tgaagaaccg caaaacggtt cggttatggt gagggatatc
     8641 cgatcccagt acgtgaatca gtacagtcat gcccgcctac gccagagtaa aaacacgcca
     8701 caacaaagag aaaaatagac gatataagtt gccatatatg cctgcgccgc gcccagtgca
     8761 ccatgcgcag ggattagcca gtgggcaaat accatcaata aagtgaactg gctgatttcc
     8821 gccagaatat aaaaccgcaa tgacgctttg gcgatcacca gataaccaaa gacataagcg
     8881 cccactttta acacatcacc caccaactgc caggcaaaga gatcgcgcat agcggtaaat
     8941 ttattcgata acagcaacca gatagcaaaa tcacgcagca accagacggt aaaactcgcc
     9001 gccgccactg ccggtaagac gaatttcagc gatttaacca cttcccgggt gatatcgcgc
     9061 ttttccgtta gccgcgacaa cgtgggcagc aaatatacgc tgaacgatgc cgtaataaat
     9121 tgcaggtagg catcggaaat actgctcacc ccttgccaga tccccacttc atcccagcta
     9181 tactgcgccg ccagcagttt acgcatcatg atgtaagcaa caggcaaggt caccgacgta
     9241 atcaacgcca tgagcgtaaa tttgctcaac tgccctgcca gaccgttatc ccaactgggt
     9301 ttcagatagc ttaacgggat agcgccacgc ttaattaaca tgacggcggc aggcattacc
     9361 accagcgcgg gaatcagcgc cagaccaagc aacgcgcctt cataaccgcc aaaacggtaa
     9421 ctgacataat aagcaataac gccaatcagg ctgccgacaa tcagcgataa cgcattaccc
     9481 gcggcatcgc gaaagccttt cattagcgcc agcaacaggt tgccccaggc gatccccatt
     9541 tgcaccagcg ccactaaacg caccagcccc tgatagtcgg tattaccaaa caatccctgg
     9601 ctgattggcg cagccgccag cacaaaaacc agcgccatca gcgtggagaa gccaagcacc
     9661 atcgctgatg aagtgccgac cacgcggcgc agctgttgcg gattatcatg gtactgggca
     9721 acgtatttgg ttacaccgtt aaagatgcca gccccggcaa gcacgccgag cacggtaatc
     9781 aactggcgga aatttgccgc cagcccaagc cccgccggac caaatgacac cgccagcaac
     9841 ttaccgacca gtaacccggc accaatcttg accagtgtac tggccgccgt ccacaaggac
     9901 gcttttgcca acgacatatc aggagaagta gttcaacaaa gtcgcaatta ccgtacgctg
     9961 attgacgggc gacaggttgt agaacagcgg caggcgcagc aggcgctcgc tctctttggt
    10021 ggtgtagcga tcttcaccgt ggaactcacc aaagcgttcc cccgcagggc aaccgtgcag
    10081 cggaatgtaa tggaacaccg ccataatttc cgcttctttc agaaagttaa tcaacgcgct
    10141 ccggtcatca atatcccgca gtttaatgta gaacatatgc gcgttctgca cgcagccatc
    10201 gggaatcgac ggcagctcga tacgcccggc tttcgccaga ggcgctaacg catcgtagta
    10261 gttttgccac agcgccagac gttgctggtt gatacgatcc gctgcttcca gttgcgccca
    10321 caggtacgca gcttgcagat cggacatcaa atagctggag ccgatatcgc gccaggtata
    10381 tttatcgacc tgaccacgga agaactggct gcggttagtg cccttttcac ggatgatctc
    10441 ggctcgttcg attaacgctt tatcgttaat cagcgtcgcg ccgccttcac cacccgccgt
    10501 gtagtttttg gtttcatgga agctaaagca gccaatatga ccaatggttc ccagtgcacg
    10561 ccctttgtaa gtggacatca cgccctgagc ggcatcttcc accacaaaca gattatgctt
    10621 tttcgccaac gccataatgg tgtccatttc gcaggccacg cccgcgtaat gcaccggcac
    10681 gataacgcgc gttttgtcgg tgatcgccgc ttcaatcaac gtttcgtcga tgttcatggt
    10741 gtccgggcga acatccacaa aaacgatttt tgcgccacgc agcacaaagg cattggcggt
    10801 ggagacaaag gtgtagctcg gcatgatcac ttcatcgcca ggctggatat cgagcagcag
    10861 cgccgccatc tccagcgaag cggtgcagga cggcgtcagt aacactttgg cgctgccaaa
    10921 acgttgctcc agccactgct ggcagcgacg ggtaaaaccg ccatcgccac acagtttgcc
    10981 gctacccatt gccgactgca tatagtcgag ttcggttccc accaccggcg gtgcgttaaa
    11041 tggaatcatg tgatcacctg tataaccagt acgcggtgct ttctacattc gcaccacttt
    11101 gtatgtatcg tttaagcgcg gcggtgttgc ccatttgggt cgccacccgc aaagttgttt
    11161 taccgcgagc atacgcccag tttagcgccg tttgcatcag ctcagcacct gcaccgcgtc
    11221 cagccagcag gccaattcgc gcatctgtcg cattgagttc ccgtaaagag acataaccgc
    11281 gaatatcgcc ggacgccgca cgtaaaatca gacattgatg atcaaaggtg ccgcgcacgg
    11341 cattttcaat ccactgtgca taaaagcgac tgctggcgtc aggcgcatac catggcgcac
    11401 gaaaacggct ttgcgcaaat gcggcgctgg ctaactgacg taatgcggga atatcggtct
    11461 cttgtgccac tacagcaccg ctatcactgg cattgttcac gggtagcgcc aaatcaactt
    11521 caccttctac cagggagaat cccagctgtt gcagggcatc cagttcaccc gtatttgatg
    11581 ccgcaatttt ggcctgcacc cgtgaccacg gcgctaacgc gtctggcgtc aggagcggtg
    11641 cttcagacgt aatgcgcacg atggcgctgt taacaccaaa gaagtcgttt tcccaggtta
    11701 gtggctcaat actggcgcgg acgggcacga agtaactcca gcagatattg gccgtagcca
    11761 gttttcgcta atgaactggc agcacgcttc acaccctcgt catcgagcca gccgttacgc
    11821 caggcgatct cttccaggca ggcaatctta aagccctggc gtttttctac tgtctgcaca
    11881 aaggtactgg cttcaatcag gctgtcgtga gtgccagtgt ccagccaggc aaatccgcgc
    11941 ccgagcagtt caacggtcag gttgcccgcc tcgaggtaca tctggttgat ggaggtaatc
    12001 tccagttcac cacgctccga cggcttcacc tgctttgcgt actccacgac tttactgtcg
    12061 tagaaataaa gcccggtcac cgcccagttt gacttcggct gttttggctt ttcttccagc
    12121 gagatagcgc ggaaattgtc gtcaaactcc accacgccaa agcgttccgg gtccatcacc
    12181 tgatagccaa aaaccgtcgc cccttccgtg cgcgccgcaa catgacgcag cttcggactg
    12241 aagccctgac cgaagaagat gttatcgccc agcaccagac aagaaggttc accattaagg
    12301 aaggtttcac cgatgataaa ggcctgcgcc agaccgtccg ggctgggctg ttcggcatat
    12361 tccagctgga taccgaactc actaccatcg cccagcaggc gctggaaata acctttatct
    12421 tccggcgtag tgatgatgag aatttcgcgg ataccggcca gcatcagcac cgacagcgga
    12481 tagtaaatca ttggcttatc gtaaatcggc aatagttgct tcgatacgcc gcgcgtaatc
    12541 ggatgcaatc gggtaccgga accgcccgcc aggataatac ctttcatgtt tgcctccgct
    12601 gaaaattagc ctttcagacc taaacgctcg ccctgatagc tgccgtcctg cacctgcttc
    12661 caccagcttt cattagccag ataccactgc accgttttac gcattctact ttcaaaggtt
    12721 tcctgcggca gccagccaag ttcatgggca attttcgaag catcaatggc ataacgcaga
    12781 tcatgccccg gacggtcagc gacaaaggtg atcaggtcac gatagtgcgc cacgccgtgc
    12841 ggcttgttcg gagccagttc ttccagcagc tcgcaaatgg tttccacaac atcgagattc
    12901 ttacgctcgt tgtgaccacc aatattatag gtttcaccga ctttcccggt ggtcgccacg
    12961 caatacagcg cgcgggcatg atcttccaca tacagccagt cacggatttg ctgcccgttg
    13021 ccatataccg gcagcgattt acccgccagc gcgttgagga tcatcagcgg gatcagtttt
    13081 tccggaaagt ggtaaggacc gtagttattc gagcagttgg tgataagcgt tggcagacca
    13141 taggttcgca gccaggcgcg caccagatgg tcgctgctgg cttttgacgc ggaataaggg
    13201 ctgctcggcg catacggcgt ggtttcggtg aagaaatcat ccgtcgagtg caggtcacca
    13261 tatacttcgt cagtggagat atgatgaaaa cggaacgctg attttttatc ttccgtcagc
    13321 gcattccagt aagcccgcgc cgcttcaagc aatgtataag tcccgacaat gttggtttca
    13381 ataaacgctg ccgggccgtc aatagaacgg tcaacatggc tttcggctgc cagatgcatg
    13441 acacagtctg gctgatgctc agtgaatacg cgcgccagtt ctgcccgatc gcagatatca
    13501 actttctcaa aggcaaagcg ctcgctttgc gcgaccggtg ccagcgacat caggtttccg
    13561 gcgtaggtca gcttatcgac cactaccacc gcatcgctcg tttcgttgat gatataacgc
    13621 accagcgccg agccaataaa cccggcacca cctgttatca gaatttttct catcagcgcc
    13681 agactccttt ggcatcgacg acatactgct gatggacatt gtcgccattg ataactttga
    13741 actgactatg atcgaccagc atcaccagca cgtctgccgt tgccagcgcc tcgtcaagct
    13801 gcgccagagt acaaagcccg gtcagttttt tcggcaactg gtggatgtta ggctcaacaa
    13861 ccagcgtttc gccgctatgc cactgggcga tcagttcagc gatttccatc gccgggcttt
    13921 cgcgcaggtc atcaatattc ggtttaaacg ccagaccaaa gcaggcgatt ttcagttcac
    13981 tggcgcgttt atcggtagcc gccaggcaat cagccaccgc cgctttcacc tgatcgataa
    14041 cccagaacgg tttgtgatcg ttcacttcgc gcgcggtacg gataagccgc gcctgctgcg
    14101 ggttctgtgc cacaataaac cacggatcaa cagcgatgca gtggccgccc acgccagggc
    14161 caggctgaag aatattgacg cgagggtgac gattcgccag gcgaatcagt tcccagacgt
    14221 taatcccctg atcggcacaa atcagcgaca attcattagc aaaagcgata ttcacatcgc
    14281 ggaagctgtt ttcggtaagc ttacacattt ccgccgtccg cgagttagtg acgacacact
    14341 caccttcgag gaaaattttg tacagttcgc tggcgcgggc cgaacaaacc ggcgtcatac
    14401 caccaatcac gcgatcgttt ttaatcagct cgaccataac ctggcccggt aacacgcgtt
    14461 ccgggcagta agcaatgttg acgtccgcct gctcgcccac ctgctgtggg aaagtgagat
    14521 ccggacgcat ctctgctaac cattctgcca tcttctcggt tgaccccacc ggcgaggtgg
    14581 attcaaggat caccagcgcg ccttttttca gcactggcgc aatggagcga gcagccgatt
    14641 caacgtaggt catatctggc tcatgatcgc ctttaaacgg cgtgggtaca gcaatcagcc
    14701 aggcatccgc ttcaactggc gtcgtgctcg ctcgtaaaaa accgccttct acggcagttt
    14761 ttactacgct cgccaaatca ggctcgacga tatggatttc gccacgattg atggtatcaa
    14821 ccgcgtgttg gttgatatcg acaccaatca cctgtttttg ccgtgaggca aacgctgctg
    14881 ctgtaggcag cccgatgtaa cccagtccga taacagaaat ggtcgcaaaa ctcatagtga
    14941 tatccgatta ttttttaacg cttccagaat gcgagagcat gcctgaccat caccatacgg
    15001 gttatgggcg cggctcatag cttgatattc gttttcgtct tttaaaagac gcgtcacttc
    15061 ctcgacaatt cgctgcttat ccgtgcctac cagacgcacc gtacccgccg tcaccgcttc
    15121 cggacgctca gtggtatcgc gcatcaccag caccggtttc cccagcgaag gcgcttcttc
    15181 ctgaatgccg cctgagtcgg tcaaaatcag ccaggcgtgg ttcatcagcc agacaaacgg
    15241 taaatactcc tggggatcga tcagaatgac atttttcaca tgccccagaa tgcgattgac
    15301 cggctctctg acgttcgggt tgagatgcac cggatagaca atctggatgt cctggtgcgt
    15361 ggtggcgatg tctgccagcg cgtggcagat ttcttcaaag ccacgaccga aactttcacg
    15421 cctgtgaccg gtcaccagaa tcatcttttt atcggggtcg ataaacgggt aatttgccgc
    15481 cagttctgaa cgcagcgtgt cgctgctcat cacctggtca cgcacccata acagtgcatc
    15541 aatgactgta ttaccggtaa tgaagattcg gctatccgca acgttttcac gcagcaagtt
    15601 ttgccgggaa gtttcggttg gagagaagtg atacatcgcc agatgcccgg tcaatgtacg
    15661 gttagcctct tccggccacg gcgaatagag atcgcccgtg cgtaaaccag cctcaacgtg
    15721 accaacagga atacgctgat aaaacgccgc caggctggtt gccagcgtcg tcgttgtgtc
    15781 gccgtgaacc agcacgacgt ctggtttgaa ctcggcaaga ataggtttta gcccttccag
    15841 aatccgacag gttatctctg tcaggccctg tcctggctgc attatgttga gatcgtagtc
    15901 aggtacaatg gaaaagagtt tcagcacctg atcgagcatc tcccgatgct gcgcagtgac
    15961 gcaaacttta gcctcaaaaa aaggatcttt tgccaacgca tgcaccaacg gagccatctt
    16021 gatggcttcc gggcgcgtac caaatacagt cagtactttc acatcgattc tcttcgaata
    16081 agcggcgagc gcctttgcgc tcaccgcagc agtgttgcta tttcgagcaa cggcgggtta
    16141 atgcgacacc agccccgatc agccccccga caatgcccca cataatcatc aggaaggcac
    16201 gacgtgggct atcgcgtttt accggttctt ccggcgtacg caaatagcga taggtctgaa
    16261 aacgcggatc cagggttgga ccaacattca gggtgtttaa catggcccga ttctgatcat
    16321 agtcgagatc aaaggccgga ccgacggcct gtaaattttc cagtcgagcc tggagcattg
    16381 gacgcccaag caggaacatt tctgaatcag gtaattcctc ggcaggcaca tctgtcgcac
    16441 tgcgcgaaat attatgctgc tcagcaattt tcagcgcctg ctcaatgctg ttcatccggc
    16501 ggtcgtagat ggctttcgcc acctcttcct gacgcttcac ctgagctttc atctggatgg
    16561 tgcgtgccgc ccatgcgcct ttcagctcat cattcagatg gctggctgca cgctggctgg
    16621 caaaagcaac atactgacgt aacaggttat tagcgtcagg cgcggtttcg gcaataagct
    16681 tcacgctgtc attgaccgcg cgggtaaagt ctccggggat aaactggatg ttgttaatca
    16741 tttcatccag caacgccgca tcggctttgc tgttgcccac catccgctgc ttgtaatagt
    16801 cggtttgcag ccagaactct ctgcgggtat cccacgaggc cagttgcata acaaactctt
    16861 tataggcttc gtccatgacc gatggttggt cggcagaagc catgtttgaa cggacatcca
    16921 ggttacgcaa aaattgctgc tgcgagtaat atccccccag catattcacc gttggacgat
    16981 cggtaatcgc cgtcgagctc cactcctgac gagcaaaaaa agtatacgcc agcgcgatta
    17041 acgcaaacgc cagccccatg ccaataatcc atagcttccc agcccacaag gtacgaaaca
    17101 acccacgaat atccagttca ttttcagcgt cttcggccgg tttcccaggc attggttgtg
    17161 tcatcacatc ctcatttatt tggttaaatt ggggctgcca ccacgatttc tacgcagtct
    17221 gcgttttacg cgcttaataa agcgagcaac tttccaggca cgcttaatgc aatatccata
    17281 gaggaagaat gctagcaaaa agagcaccag catgacccac tccgggacaa aatgagaata
    17341 ttctgccagc acgccaatgg aagcgagcag tgctgcggca agggtaatca gcacaaacgc
    17401 ctgacgggaa gtaaacccgg cacgcatgat caaatggtga atatgctgac ggtcaggaga
    17461 gaatgggctc atgcctttac gcaggcgacg gtacataatc gccaccatat ccattagcgg
    17521 aatggcgatt atccacaaag cggtaaccgg gctgatggga tgggttttac cctgggtcgt
    17581 ttcgagcagg atccagatca cggtaaaacc aatcagcgta ctgcccgcat cacccataaa
    17641 gactttgtag cggcgaccca ggataccaag gttaagcatg atgtatggca ggatggcggc
    17701 gatcatcgca aagcaccaga ttgcgaggct ggtttgcccg tcgaaccaca aaatcatacc
    17761 gattgctgca aacgagacgc aggataaccc gcccagcaag ccatcaatgc catcaaccat
    17821 gttgaacgca ttaatggccg cccagacggc aaatagcgtc aggaagtaac caaacggtcc
    17881 gagcaccatc tcccaggagc caaagatata acccaggcta ctgagataaa gtttgccgaa
    17941 caccatcata acaatgccaa cagcggcctg tatggtggca cggattttta cgctgatatc
    18001 aaaacggtca tccagcgcgc caatgaaaac aagcacaccg gcacaagcga gatagagaga
    18061 tgcatgcgga atatagtaat cgacaattcc gaacgtgaag caaatccctg cgtaaaccga
    18121 aatcccccca acgagaggta tcaatccctg gtgacgtttg cggaagtttg gtttatccac
    18181 taaaccgact ttttttgcca ccttacgggc aaaaaacaga aacagtgtcg tgaataaaaa
    18241 aatactgatg agatcagtac tcactgtcag taaattcaca atgcatgctc tcagagaaaa
    18301 ttattagcag aagtataacc acgaagacct ttattcagaa gggaaagtcc gttccgaacc
    18361 caccagtcct gtgcaaaaat taaccatttg atatgcaaat tgcataacat gccagcaaat
    18421 tccaggaatt ttcctaacat cgccagcgcg gcattaagat tacagatata aaagcaaaac
    18481 gccacgtaaa cacgtggcgt ttttggcata agacaaattt atgagcgttt catcatttcg
    18541 aagaaatcgt cattggtctt ggtcattgcc agtttattaa tgaggaattc cattgcatcg
    18601 atttcgccca tcgggtgaat gattttgcgc aggatccaca ttttctgcag ttcttcctga
    18661 gtcgtgagca gctcttcttt acgggtaccg gaacggttgt agtcgatagc cgggaagacg
    18721 cgtttttcag cgatcttacg agagaggtgc agttccatgt tgcctgtacc tttaaactct
    18781 tcgtagataa cttcgtccat tttagaacca gtatcgataa gcgccgtcgc gataatggtc
    18841 aggctgccgc cctcttccac gttacgcgcc gcaccaaaga agcgtttcgg acgatgaagg
    18901 gcattggcgt ccacaccacc ggtcaacact ttacctgacg ccggaacaac ggtgttgtaa
    18961 gcgcgcgcca gacgagtgat ggagtcgagc agaatgataa cgtctttctt gtgctcaacc
    19021 aggcgtttcg ccttctcgat caccatttcc gcaacctgaa cgtggcgaga tgcgggttcg
    19081 tcaaaggtag aagcaacaac ttcacctttt accagacgct gcatctcggt tacttcttcc
    19141 ggacgttcgt cgatcagcag aaccatcagc acacaatccg ggtggttgta agcaatgctc
    19201 tgagcaatgt tctgcagcag catggtttta cctgctttcg gcggtgccac aatcagacca
    19261 cgctgaccac gaccgatagg tgatgccaga tccagtacgc gagcggttaa atcttcggta
    19321 gaaccgttac cacgttccat acgcagacga gagtttgcgt gcagcggggt taagttctca
    19381 aagaggattt tgttgcgggc gttttcaggt ttgtcgaagt taacttcgtt aactttcagc
    19441 agcgcaaaat agcgttcacc ttctttcggc gggcgaatct taccagagat ggtatcacca
    19501 gtgcggaggt tgaaacggcg gatttggcta ggggaaacgt agatgtcatc aggaccggcg
    19561 aggtaggagc tgtctgcgga acggaggaaa ccaaatccat cctgcaatat ctccagtacg
    19621 ccatcaccaa agatatcttc gccactcttt gcgtgctgct tcaggatggc aaaaataatg
    19681 tcctgcttac gcatacgagc caggttttcc agccccatat tttcgccgag agtgatcagc
    19741 tcagaaaccg gcgtattctt taattcggta agattcatag tggtgtgagt tcttaaactt
    19801 ggggtaaatc tcgaacttaa tgttgtgaat ggtatggcag gatcatccat gcctgtttac
    19861 ggtcatcgac tcatgtcttt tcgctgcctg gtcacaggag agtacgcaga actgaaacga
    19921 caagacggat tgagtgacga gccagaaatc tgttcacttc gcatttaaga taaaacggga
    19981 agcgttggaa attacaagat tcaaacttaa taaggtatgt ttaatacgaa gtcaacacta
    20041 agttagcatg actcacgccg ggcgtccagt ttttagcgac ggggcacccg aacatgaaat
    20101 tcccttacgc caggttagcg tcgaggaact ctttcaactg acctttagac agtgcgccca
    20161 ctttggttgc cgccacttca ccgtttttga acagcagcag agtcgggata ccacggatgc
    20221 catatttcgg cgcagtgcca gggttttgat cgatgttcag ttttgcaacg gtcagtttgc
    20281 cctgatattc gtcagcgatt tcatccagaa tcggggcgat cattttgcac ggaccgcacc
    20341 actctgccca gaaatcgacg aggatcgccc cgtccgcttt gagtacatcc gtgtcaaaac
    20401 tgtcgtcagt caggtgaata attttatcgc tcatatataa ctccacagga ataagcctgg
    20461 cgtgttggtt tcgttgttgg tgtaacatta accaactaaa ggttgacttt atttcaccgg
    20521 atacgctttc gtaaagcaat agtaagctga tattctacca cactatgagc aaaacacatt
    20581 taacagaaca gaagttttcc gacttcgccc tgcatccgaa ggttgtagaa gcccttgaaa
    20641 aaaaagggtt tcataactgt acgcccattc aggcactggc ccttccgctg acgctggcgg
    20701 gtcgtgacgt agccgggcag gcgcaaaccg gtaccgggaa aacgatggcg tttcttacgt
    20761 caacgtttca ttatcttctc tctcatcctg cgattgccga tcgcaaggtg aatcagccgc
    20821 gtgccttaat tatggcaccg acgcgtgaac ttgccgtgca gatccatgcc gatgcggaac
    20881 cgctggcgga agctaccggc ctgaagctgg gtctggctta cggtggtgat ggttacgaca
    20941 aacagctgaa agtgctggaa agcggcgttg acattctgat tggcaccacg ggtcgtttaa
    21001 ttgactacgc caagcagaac cacattaacc tcggtgccat tcaggtagtg gtactggacg
    21061 aagccgatcg catgtacgat ctgggcttta ttaaagatat ccgctggctg ttccgccgta
    21121 tgccgcctgc aaaccagcgc ctcaacatgc tgttctccgc cacgctttcg taccgggtac
    21181 gtgaactggc gttcgagcag atgaacaatg ccgaatatat tgaagtggaa ccggaacaga
    21241 aaacgggcca ccgtattaaa gaagagcttt tctacccttc taacgaagaa aaaatgcgtt
    21301 tgctgcaaac gctgatcgaa gaagagtggc cagacagagc gattattttc gccaatacca
    21361 aacaccgttg tgaagagatc tggggccacc tggcggcaga tggtcatcgt gtcggtttgt
    21421 tgacaggcga tgtcgcgcag aaaaaacgtc tgcgtattct tgatgaattt acccgtggcg
    21481 atctggatat tctggttgcc accgacgttg ccgcgcgtgg tttgcatatt ccggcagtga
    21541 cacacgtctt taactacgat ttacccgatg actgtgaaga ttacgttcac cgtattggtc
    21601 gtacaggtcg cgcaggcgca agcggtcact ctattagcct ggcgtgtgaa gagtatgcat
    21661 tgaatttgcc tgctattgag acctatattg gtcactcaat tccggtaagc aaatacaatc
    21721 cggacgcatt gatgaccgat ctgccaaaac cgctgcgcct cacgcgcccg cgcacaggca
    21781 atggtccgcg tcgtactggc gctccgcgta atcgtcgtcg ttcaggttaa ataaaaaatg
    21841 tcatactcaa aatcattcaa actgtatcaa gatggcaaga gaatgaatcc cgatgagctt
    21901 actgtagtaa gtgattcagg tgaaagaatg cagccaacac agagacagat tgaaggatga
    21961 agagtatggg ttccacctcg tcgctgtatg cagccattga tctcggttcg aatagttttc
    22021 atatgctggt tgtgcgcgag gtggctggaa gcatccagac gctgacgcga attaaacgca
    22081 aagtgcgtct ggctgctggc ctgaatagcg aaaataccct ctctaatgaa gcaatggagc
    22141 gcggttggca atgtctgcgc ctgtttgctg aacgtctgca agatatccct ccctcgcaaa
    22201 ttcgtgttgt cgccacggcg acgttacgcc ttgccgtcaa tgcgggtgat tttattgcca
    22261 aagcgcagga aatcctcggt tgtccggtac aggtgatcag cggtgaagag gaagcacgtc
    22321 tgatttatca gggtgttgct cacaccactg gcggtgccga tcagcgcctg gtagtggata
    22381 tcggcggtgc cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc tcgttgttca
    22441 gcctgtcgat gggctgcgtc acctggctgg aacgctattt tgccgatcgt aatctggggc
    22501 aggaaaattt tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg gttgccgatg
    22561 aattacggta tcacggctgg aaagtgtgcg ttggtgcttc cggcaccgtg caggcgttac
    22621 aggaaatcat gatggcacag gggatggatg aacgcattac cctggaaaag ttgcagcaat
    22681 tgaaacagcg agccattcat tgcggtcggc tggaggaact ggagattgac gggctgacgc
    22741 tggaacgtgc gttagtgttc ccgagtggtc tggcgatcct gatcgctatt tttaccgaac
    22801 tgaatattca gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg gtctacggca
    22861 tgttgcatct ggccgtcgag caggatattc gcagccgtac gctgcgtaat attcaacgcc
    22921 gctttatgat cgatattgat caggcacagc gcgtagccaa agttgcggct aacttcttcg
    22981 atcaggtgga aaacgaatgg catcttgaag caataagccg cgatttgctc atcagcgcct
    23041 gtcagcttca tgaaatcggc ctgagcgttg acttcaaaca agcgccgcaa cacgctgctt
    23101 atctggtgcg taatctggat cttcccggtt ttacccccgc acagaaaaaa ttgctggcga
    23161 cgctactgct caaccagact aatccggtcg atctctcatc gctgcatcag caaaatgccg
    23221 taccgccgcg cgtcgcagaa caactctgcc gtttactccg cctggctatc atttttgcca
    23281 gccgtcgccg tgacgatctc gtgccagaga tgacattaca ggctaaccat gaactgttga
    23341 ccttgacgct tccgcaaggt tggctaaccc aacatccgct gggtaaagag attattgctc
    23401 aggaaagcca gtggcagagc tatgtccact ggccgctgga agtgcattaa tgagtaagtg
    23461 ccggatgcga tgctgacgca tcttttccgg ccttgattat ttccctcgtt ttgccgccat
    23521 catcgctttc agattcgcca gatggctttg ccctttctgc atccgttctt cggcgctgac
    23581 cactttgcgc tcctgttccc aaatcagatc atcctgcggc agctccagca aaaagcggct
    23641 cggctccggg cgcaccagtt cgccgtactg acggcgttct ttacacagcg taaaggtcaa
    23701 ttccttctgg gcgcgggtaa tgccgacata ggccagccgc cgctcctcat cgatattatc
    23761 ttcatcgatg ctgctctggt gcggcaaaaa cccttcttcc ataccgacca tgtagacata
    23821 aggaaactcc agtcctttcg acgcgtgcag agtcatcagt tgcacctgat ccagctcttc
    23881 ctcactctcg ccgcgctcca tcatgtcgcg caaagtaaag cgcgtcacca cctgggtgag
    23941 cgtcatcggc tcatccagtt cgctgccttc cagcatctcc gtcatccagc taaacagttg
    24001 gttgacgttc ttcatgcgca tttcggcagc tttcgggctg ggcgatgttt cgtatagcca
    24061 ggattcataa tccatgccgt ggatcagatc gcgcaccgcg gcaatcggat cccgctccgc
    24121 cagacgctgg atttctgcca gccagtgagt aaagcgggtc aacgcctcat aaccacgtcc
    24181 gctaagcgtc tgactcaatc ccatatcaaa gctggcggta aacatgcttt tattgcgcgt
    24241 catcgcccat tcacccaact ttttcagcgt cgccgggcca atctctcgct tcggcgtgtt
    24301 aacgatccgc aaaaacgcgc tgtcatcatc cgggttagtc agtacgcgca gataagccag
    24361 caagtccttg atttccggac gggagaagaa cgacgtgcca ccagatattt tgtacgggat
    24421 gcggttttgc atcaggaatt tttcaaatac ccgcgactga tggttgccgc gataaagaat
    24481 ggcgtaatct ttgtactgcg ttttattgac gaagtgatgc gcaatcagtt caccagtgac
    24541 gcgctcggct tcatgttctt cgttattcgc gcttaaaact tttagctctg tgccgtaacc
    24601 cagttcggag aacagacgct tttcaaagac gtgcgggtta ttggcgatca ggatgttcgc
    24661 cgctttcaga atacgcccgg aagagcgata gttctgctca agcttaatca ccttcagcgc
    24721 cggaaaatcc tgactcagca gcaccaggtt ttgcggacgt gcaccgcgcc aggagtagat
    24781 cgactggtca tcgtcaccca ccacggtaaa gcgcgcgcgg ctgcccacca gcagtttcac
    24841 cagttcatac tggctggtgt tggtgtcctg atactcatcc accagcaggt agcgaatttt
    24901 gttctgccag cgttcgcgga cttcttcatt gcgttgcagc agcaacgtcg gcaataaaat
    24961 caggtcatcg aagtcgagaa cgttacaggc tttcaggtgt gcatcataca gcccataaca
    25021 atgggcaaaa atacgatccc gctcgccctt tgcctctgcc gccgcctgcg ccggagtttt
    25081 gagatcgttc ttccagttag agatggtcga aatcagttgt tgcaggagaa ctttgtcatc
    25141 ttcaatcagc ccctcggtca actctttaag caaagcaagc tgatcggtat cgtcaaacaa
    25201 cgagaagttc gctttcatcc caagcgccgc atactcgcgt ttgatgatat ccagccccaa
    25261 cgtatggaaa gtggagatca tcagcccacg cgcctctttg cgccccagcg tctgccctac
    25321 acgctctttc atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga tatgtcgcgc
    25381 ctgataaccg caaccgcgga tcagatgggc gattttattg gtgatcacac gagttttacc
    25441 ggaacccgcg cccgccagca ccaggcaggg gccggtaacg aattcgacag cttgttgttg
    25501 gccggggttt agacgcatag gtgtattgct caatcttcga acggggggag gattgtagca
    25561 gaatcgcatt tgaatgtgac cgcgtgtaag ccgctaaccg gttggctacg gatgtttaaa
    25621 tcagagagag aagaccgaaa tcggagttca tcgtgaatcc attagactta gaaaatatcg
    25681 ggtcgtaacg ttgtgttatt actttttcag agtttactaa ggagaggttt actcgtggcg
    25741 attaatttca gccctaaggt tggtgaaata ctggaatgca actttggtaa ctatcctgtt
    25801 agccaaaatg gaccgttcag cacgacgtat tacgatggcc gcattcctcc tgaaatgata
    25861 aaaaacaggt tacttgtcgt gctcaacgga aaaattaatg gcaacgcatg catcgtcgtt
    25921 ccattatcaa ccactcgcga ccatgacaag ttaaaacgtg gaatgcatgt tgaaatagcc
    25981 aggaatgtta tcaatgacct gcaattcttt gatcagcaga ttcgttgggc caaaaccgac
    26041 ctggtacaac aagtcagtcg aaacagatta aacagagcaa gaacttacag agggtatctt
    26101 aatcaatgtt tgccacatga gttggttgca gacatacaga gggcggtcat caagtcaatc
    26161 aatgccatat cgctcataaa ttgacaataa aatcaccatc cccgataatg actttgtagc
    26221 ccatagaggc cctaagtttt ctccccttga gggacctgcg aagcccggat tttccgggct
    26281 tttgccatat atgtaccagc ccctccctga ctgacaatta cgttagcatc tgcggatcat
    26341 gctaaaatcg ccgcctgaca attattcact caaggcacga tcatggcaaa aacagcagca
    26401 gcactgcata tccttgtaaa agaagagaaa ctggctctgg atcttctcga gcagattaag
    26461 aacggggccg attttggcaa gctggcgaag aaacactcca tttgcccatc aggcaaacgc
    26521 ggcggtgatt taggtgaatt ccgccagggt cagatggttc cggcgttcga taaagttgtg
    26581 ttctcttgtc cggtactgga gccaaccggc ccgctgcaca cccagttcgg ttaccacatc
    26641 attaaggtgc tgtaccgtaa ctaatagcaa ggccttctcc gggagaaggc cttgaactct
    26701 gaatcgctat taacccgcaa ctgcaatacg tttcatatca gtcatgtagc cgcgcagttt
    26761 cttacctacc tgctcaatcg catggctgcg aatcgcttcg ttcacatcac gcagttgcgc
    26821 gttatctacc gcaccttccg gaatagcttt acccaaatcg cccggttgca gctctgccat
    26881 aaacggtttc agcaacggca cacaagcgta agagaacaga tagttaccgt actcagcggt
    26941 atcagagata accacgttca tttcgtacag acgcttacgg gcgatggtgt tggcaatcag
    27001 cggcagttcg tgcagtgatt catagtaagc agattcttcg atgatgccgg aatcaaccat
    27061 ggtttcaaac gccagctcaa cgcccgcttt caccatcgcg atcatcagta cgcctttatc
    27121 gaagtactcc tgctcgccga ttttgccttc atactgcggc gcggtttcaa acgcggtttt
    27181 gccggtctct tcacgccagg tcagcagttt cttatcatcg ttggcccagt ccgccatcat
    27241 gccggaggag aattcgccgg agatgatatc gtccatatgt ttctggaaca gcggtgccat
    27301 gatctctttc agctgttcag agagcgcgta agcacgcagt ttcgccgggt tagagagacg
    27361 gtccatcatc agggtgatgc cgccctgttt cagcgcttcg gtgatggttt cccaaccgaa
    27421 ctgaatcagt ttttctgcgt atgccggatc ggtgccttct tccaccagct tgtcgaagca
    27481 cagcagagag ccggcctgca acataccgca cagaatggtt tgctcgccca tcaggtcaga
    27541 tttcacttcc gcaacgaaag aggattccag cacgcccgca cgatgaccac cggttgccgc
    27601 cgcccatgct ttagcgatcg ccatgccttc gcctttcgga tcgttttccg ggtgaacggc
    27661 aatcagcgtc ggtacgccga atccacgttt gtactcttca cgtacttcgg tgcctgggca
    27721 tttcggcgca accatgacta cggtgatgtc tttacggatc tgctcgccca cttcgacgat
    27781 gttgaaacca tgcgagtaac ccagcgccgc gccgtctttc atcagtggct gtacggtgcg
    27841 cactacatca gaatgctgct tgtccggcgt caggttaacc accaaatccg cctgcgggat
    27901 cagttcttcg taagtaccca ctttaaaacc attttcggtc gctttacgcc aggacgcgcg
    27961 cttctcggca atcgcttctt tacgcagagc gtaggagata tcgagaccag aatcacgcat
    28021 gttcaggccc tggttcagcc cctgtgcgcc acagccgacg atgaccactt ttttaccctg
    28081 aaggtagctc gcgccatcgg cgaattcgtc gcggcccata aagcgacatt tgcccaactg
    28141 tgccagttgc tggcgcaggt tcagtgtatt gaagtagtta gccatggtga ttcctcgtga
    28201 tgttgtgctt cttattgttc ggttcgcgtt tgcgagattg aattcactat atgacaggaa
    28261 atttattgcg gaaattgata tattcacaac gtcacattgc aatttatgca acgtcaacat
    28321 tgagggctgc gcctgtggat ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc
    28381 gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc tcacggcaga
    28441 ttcagcgcct ggaagaagat ctcggccaac cgctgtttgt gcgcgataac cgcacggtaa
    28501 cgctgacgga agcgggcgaa gaactgcgcg ttttcgccca gcaaacactg ttgcagtatc
    28561 agcagctgcg ccacaccatc gatcagcaag ggccgtcgct ctctggcgaa ttacatatct
    28621 tctgctcggt gaccgctgcc tacagccatc tgccgccgat tctggatcgc ttccgcgcgg
    28681 aacatccgtc ggtggagatc aaactcacca ctggtgatgc ggcagatgca atggaaaaag
    28741 tggtcacggg agaagcggat ctggcgattg ccgggaaacc ggagacgcta cccggcgctg
    28801 tggcgttttc gatgctggag aatctggcgg tagtgctgat tgcgcccgcg ctgccctgcc
    28861 cggtgcgtaa tcaggtctcg gtagagaagc cggactggtc aacggtaccg tttatcatgg
    28921 ccgatcaggg gccagtacgc cgccgcattg aactgtggtt tcgacgcaat aaaatcagca
    28981 acccaatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg gtggcgctcg
    29041 gctgtggtgt ggcgctgttg ccggaagtgg tgctggaaaa cagcccggag cctgtacgca
    29101 accgggtaat gattttagat cgcagcgatg agaaaacgcc gtttgaactg ggcgtgtgtg
    29161 cacaaaaaaa gcggctgcat gagccgctga ttgaggcgtt ctggaggatt ttgccgaacc
    29221 acaaatgacg ttgtcgcgcg agtaggcctg ataagcgaag cgctatcagg catttttccc
    29281 taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca atcgtagccc
    29341 agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa cgccgccagt
    29401 acacgcccgt agtcggtacc gtgactgcga taatggaaca gagaaatatt ccagtgcgta
    29461 cccagcgtgt tgaggaagcg cagcagcgca cccggcgatt ccggaaattc gaagctgtag
    29521 agacgttcct gcaacggatg cgatggacgc cccccaacca tatagcgcac gtgtaacttc
    29581 gccatttcgt cgtcggagag atcaaccacg ctgtagccgc cgtcgttgag catctgcaaa
    29641 atttctttgc gctcttcgag gccacggctt aagcgcacac cgacaaagat gcaggcgttt
    29701 ttggcatcgg caaagcggta gttgaattcg gtgaccgaac gtccgccaag cagttggcag
    29761 aatttgagga agctaccttt ctcttccgga atggttactg ccagcaacgc ttcacgctgt
    29821 tcacccagtt cacaccgttc cgagacgtag cgcaggccgt ggaagttcac gttggcaccg
    29881 gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata ttttttcatt
    29941 cccgccagcg ccagcgcgcc agagggttcc gccaccgcgc gcacatcttc gaataaatcc
    30001 ttcatcgccg cacagatcgc atcactatcg acggtgatga tatcgtcgag atactcctgg
    30061 cacaaacgga aggtttcgtc gccgatgcgt tttaccgcaa cgccttcagc aaacagcccc
    30121 acgcgcggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca ggcggagtct
    30181 tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag caccgccacg
    30241 cccgcagcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg ggcgtcctgc
    30301 tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg atcgaacggc
    30361 ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttggcttt cgcttcatca
    30421 aagttcgcgc cgtggagcag cacttcgccg ccgaagccgc gcaccgcgtc gactttgata
    30481 tcggcggtgg cggttggcat gacgatcagc gccttcacgc ctaaccgtgc ggaagaaaac
    30541 gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc tttttgttct
    30601 tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg cactggctgg
    30661 cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt ttccattttt
    30721 tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc tcttaaatat
    30781 tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa ccccccagtt
    30841 tcgatttatc gcgcaccgcg cctttgtcgg cgctggttgc caggctggca taagcacgca
    30901 gggcaaagga gacttgacgt tcacgatttt tcggcgtcca ggctttgtca ccccgagctt
    30961 cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga atgccacggt
    31021 tcggaatgtc gatggcgatc aggtcgccgt cttcaatcag gccaatgctg ccgccgcttg
    31081 ccgcttccgg tgagacgtgg ccgatagaaa ggccagaggt gccgccagag aaacgaccgt
    31141 cggtgatcag cgcacaggct ttgccgaggc ccattgattt caggaagctg gttgggtaga
    31201 gcatttcctg catgccaggg ccgcctttcg gaccttcata gcggatcacc accacatcgc
    31261 ccgcgacaac cttgccgccg agaatcgctt ctaccgcgtc gtcctggctt tcgtatactt
    31321 tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggtttta acgatgcagc
    31381 cgttttctgc gaaattaccg tagagcactg ccaggccgcc gtctttgcta taggcgtgtt
    31441 ccagcgagcg gatacagcca ttggcgcgat cgtcgtccag cgtatcccaa cggcaatctt
    31501 gcgagaatgc ctgggtggta cgaatgcccg ccgggccggc gcggaacata ttttttaccg
    31561 cgtcatcctg ggtcagcata acgtcgtatt gttccagcgt ttgcggcaac gtcaggccaa
    31621 gtacgttttt cacatcacgg ttcagtaacc ccgcacgatc cagctcgccg agaataccga
    31681 taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc ggcgcgactt
    31741 tgcataactg cggaaccttg cgggaaagct tatcgatatc actcatggtg aagtcgattt
    31801 ccgcttcctg tgccgccgcc agcaggtgaa gtacggtgtt ggtagagccg cccatcgcga
    31861 tatcgagcgt catagcgttt tcaaacgccg ccttactggc gatattacgc ggcagtgcac
    31921 tatcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta ccagcattga
    31981 ggaacagctg cttacggtca gagtgggttg ccagcagcga gccgttgccc ggctgcgaca
    32041 gacccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg gagcaggaac
    32101 cgcaggttgg gcatgcggaa cgttcaacct gatcgctctg ggagtcagat actttcgggt
    32161 ctgcgccttg gatcatcgca tcaaccagat cgagcttgat gatctgatcg gacagtttgg
    32221 ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc aggcgcagag
    32281 aggccatcag catccccggg gtgattttgt cacagttaga gatgcagacc atggcgtcgg
    32341 cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga gatggcagtg
    32401 aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg gtgttgaact
    32461 ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg agatcgcgca
    32521 gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata atcggcttgc
    32581 caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcacct gccatattac
    32641 gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc ccagtgtctg
    32701 tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt aacttgatct
    32761 aaccagcccc atttatcttc ggtttcgccg gtaaagaggc caaagaaggc ttgctgaatg
    32821 cgtttggtga ccgggccaca acggccttcg ccaacctgaa taccgtctac actgcgcact
    32881 ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaggta cagggattcg
    32941 cgcgacagca cctgctcacg cacttcaatt ccctgctctt tcgccagttt gatgatggcg
    33001 tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag cacgccgtct
    33061 ttcacttcaa acaggttttc gcctgcacct tcagagatgt agccattcac atccagcgcg
    33121 ataccttcct gataaccgtg gcggcgcgct tcactaccca ccagcaggga agagagatag
    33181 ttaccaccgg ctttcgccgc ggttgggatg gtgtttggtg ctgcacggtt ccaggaggaa
    33241 accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc ccacgggaaa
    33301 gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttacgcccat gccaacatca
    33361 ccaacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg gatcacgtca
    33421 cgacaagctt ccatcagctc atcaatgctc tgcgaaaccg ggaagcgata gattttggcg
    33481 gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc tttgtgcgag
    33541 tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg cgacatcaca
    33601 tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta atcagctttc
    33661 ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg atgtggttgt
    33721 gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac taaacagtaa
    33781 gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg catcgctggc
    33841 ggcggccata ttcattgagc agacgtggaa accacgatga cgcaccacgc gtaaaacacg
    33901 ttctaaggtt tctggattga agcgagccga tacattgacc tgatgttgca tcatgataat
    33961 ttctccaaca tttctgaatt actggcgcca ggcggcacca gcggccagac gttctcaagt
    34021 tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt gtcgagtgcc
    34081 gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc gctggctaac
    34141 atgaggaaat cggggttatc agtaagggtg gtttcgctgt atcgttcctg aaaaaacagt
    34201 tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat tttcaacggt
    34261 aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga gccgtcaccg
    34321 gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaaccgc cgccggtaaa
    34381 ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg agtgtgggcg
    34441 atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac gcaatccgca
    34501 ggtttacgat ccgacagttg ttttaacaac aacggcgcgt agatagcgtc accgggatgg
    34561 tcgtaacgcc aggcatgttc atcacgcagc tgcgcgcagt gtagctgcca gtcattgata
    34621 tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa tgccacatgt
    34681 gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac actggcgtgt
    34741 ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc cacggcgatc
    34801 agcaagtcgc actcctgcac cgcgaagttc gccgctttgg tgccatgcat tcccagcatg
    34861 cccagatagt acggataatc tgcttcaact gcgcccagcc ctttcagcgt gcaggtggca
    34921 ggcatttttg tggtagcgag aaattctcgt aaagcaggaa ctgcctgcgc catacccacg
    34981 ccaccaccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg cgcttgctca
    35041 acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg ttccaggtcg
    35101 ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg acgacctgag
    35161 ctggcaacgt cgaatgcttc agccataatg cgcggcaact cttccagcga ctgcaccaga
    35221 aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg aaatgcgtca
    35281 gtgccgataa acggtgcgga cacttgaccg gtgatggcaa caacagggat agaatctaac
    35341 agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt ggcgatacat
    35401 acgccagttt tgccggtagc acgggcataa ccgatagccg ccattgccgc accctgctca
    35461 tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac cggcataatt
    35521 gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc atgtaccacc
    35581 cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag aattttgtgc
    35641 tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaacc cccgaacctt
    35701 tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc gtccaagtgc
    35761 agccccgcac ggtgggataa taatcaccac cacgctaatc acgaccaggc taatcactcg
    35821 tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc taaagagtta
    35881 ccatagattt tgcgaatagc acaagatatt ttttggccgt tttttatgat ggctttaata
    35941 atttcaatag aaattgttgt tttttaaaaa gaaaatagtg agtgaaaaaa attcaatttc
    36001 agtacgaatg ttgttccctc cctcgtagtt catcctttgt ccgctgcatt tttgcgagca
    36061 tcattccacc gccagaacaa ctcaacttca cttgcacaaa taaacaggaa tcagccacta
    36121 aaattcacga attagcgggc tacagcagcg atatcttatg cacataatgg tgactcaagg
    36181 agggcttatg tcactgtcaa ttgttcatac ccgcgcagcc ctgggagtaa atgcgccccc
    36241 aatcactgtt gaggtacata tcagtaaagg tctacccggt ttaacgatgg tgggcttacc
    36301 agaaacaacg gtaaaagaag cccgcgatcg cgtgcgcagc gccattatca atagcggata
    36361 tgaatatccg gccaaaaaaa ttaccatcaa cctggctcca gccgatctac caaaagaagg
    36421 gggacgatat gatttaccta tcgccattgc gttgctggcg gcctcagaac agcttacagc
    36481 caataagtta gatgaatatg aattagtcgg agaactggcg cttacaggcg ctctgcgtgg
    36541 cgttcccggt gcaatctcca gtgcaactga agctattaag tcgggcagaa aaattatcgt
    36601 cgcgaaagat aacgaagatg aagtggggct aattaacggt gaaggatgcc tgatagccga
    36661 tcatctgcag gctgtctgtg cgtttctgga aggtaagcac actctcgaac gcccgaaacc
    36721 aactgatgca gtatcccggg cgctacaaca tgatctcagt gatgttgtcg gtcaggagca
    36781 aggaaagcga ggactggaaa ttaccgccgc tggtgggcac aaccttttac tgattggacc
    36841 gccgggaaca ggtaaaacaa tgctcgccag ccgtattaat ggcctgttgc cagatttaag
    36901 caatgaagag gcactggaga gcgctgcgat attaagtctg gtaaatgctg aatcagtaca
    36961 aaaacaatgg cggcagcgcc cgttccgctc acctcatcac agtgcgtcgt taactgcgat
    37021 ggtgggcggt ggtgcaattc cagggccagg tgaaatttcg ctggcgcata acggcgtgct
    37081 ttttcttgat gagttacctg aatttgaacg gcgtacactg gatgctttgc gagagccgat
    37141 tgaatccggg cagatccatc tttcacgcac acgagcaaaa ataacctatc cagcccgttt
    37201 ccagcttgtt gcggcgatga atcccagccc taccggacat tatcagggaa accataaccg
    37261 ctgcacgcca gaacagacat tacgttatct caaccggctc tcggggccct ttctcgaccg
    37321 cttcgatctc tcactggaga tcccattacc accccccggc attttgagta aaacggtagt
    37381 gccgggagaa agcagcacca ccgttaaaca acgtgtaatg gctgccagag agcgccaatt
    37441 taagcggcag aataagttga acgcctggct ggatagtcca gaaatacgca aattctgcaa
    37501 gcttgagagc gaagatgcgc agtggctgga agaaacgctg atccatctgg ggttatcgat
    37561 tcgtgcctgg cagcggttat tgaaagttgc acgaaccatt gctgatattg atcagtctga
    37621 cattatcaca cgtcagcatt tgcaggaggc agttagctat cgtgcgattg accgtttgct
    37681 catccatctg caaaaactac tgacataaaa aaagggcatt tcgccctttt tattaatcgt
    37741 cagaatcggt gtagtcttca gcaccttcaa cctgcggttt accgccggaa agggtgtgaa
    37801 aacgttttgg acgcttgata cgcgtcatat acttagacca cacgcgttct gcttctgtca
    37861 ctggctcacg ttcgccacgg catactgcta cgaagagttt ctcttcctcg gtaaccggct
    37921 cgcgtttgcc aagatccaac tcattgaagg cataaccatg acgctcaagc agttgtgcct
    37981 ctttgatggt gaaatcacca tggcgagaga atccacgtgg ataatgttta ttgtcgaaat
    38041 atcgattagt cgtcgtaaag ctttccgcca tcctgcacgc tcctaattct ttgaccgagc
    38101 tagttatggc gcggagtatt agttacgctt gacagagtgt aaaacaaaac atttaaatca
    38161 taacgacaaa taattttgcg gagagcactg tggatacgga attgttaaaa actttcctgg
    38221 aagttagccg aacgcgtcac tttggtcgag cggctgaatc gctctatctg acccagtcag
    38281 cagtgagctt tcgaatcaga caactggaaa atcaactggg tgtgaacctt ttcacccgcc
    38341 acagaaacaa tatccgttta accgctgccg gtgaaaaact actgccttat gcagaaacgc
    38401 tcatgagcac ctggcaggcc gcccgtaagg aggtggcgca tacctcacga cataacgagt
    38461 tttctatcgg tgccagcgcc tcattgtggg aatgtatgct taatcagtgg ctgggacgtt
    38521 tgtatcaaaa tcaggatgcc catacaggct tacagttcga agcgcgaatt gcccaacggc
    38581 agtctctggt aaaacagctg catgaacgcc agcttgatct tcttattacc actgaagcgc
    38641 ccaaaatgga cgaatttagt agtcagttgc tggggtattt cactttagcg ctttatacca
    38701 gtgccccttc taaactaaag ggagatctta actatctgcg actggagtgg gggccagatt
    38761 ttcaacagca tgaggcaggt ttgatcggtg ctgacgaagt gcccattctg acaaccagtt
    38821 ctgctgaact ggcacagcaa cagattgcga tgcttaatgg ttgcacctgg ctacccgtca
    38881 gctgggcgcg taaaaaaggc ggcctgcata ccgttgtcga tagcacaaca ctttcacggc
    38941 cgctttatgc catatggctg caaaatagcg ataaaaatac gttgattcgc gatctattga
    39001 aaattaacgt gctggatgaa gtgtattaat ctgaatggct ggcaaggatg ccggtagaag
    39061 gatttacttc agagagggtt atttcagata aaaaaaatcc ttagctttcg ctaaggatga
    39121 tttctggcag gggcggagag actcgaactc ccaacacccg gttttggaga ccggtgctct
    39181 accaattgaa ctacgcccct aattagggtg gcggaacgga cgggactcga acccgcgacc
    39241 ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac
    39301 aaccaccggt ttta
//
LOCUS       DAPGRN010000033        39243 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPGRN010000033 DAPGRN010000000
VERSION     DAPGRN010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.033"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            299..1558
                     /gene="codB"
                     /locus_tag="RHW33_001864"
     CDS             299..1558
                     /gene="codB"
                     /locus_tag="RHW33_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV1645368.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            1548..2831
                     /gene="codA"
                     /locus_tag="RHW33_001865"
     CDS             1548..2831
                     /gene="codA"
                     /locus_tag="RHW33_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV1645369.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(2964..3863)
                     /gene="cynR"
                     /locus_tag="RHW33_001866"
     CDS             complement(2964..3863)
                     /gene="cynR"
                     /locus_tag="RHW33_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV1645370.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAAHEQMALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            3973..4632
                     /gene="cynT"
                     /locus_tag="RHW33_001867"
     CDS             3973..4632
                     /gene="cynT"
                     /locus_tag="RHW33_001867"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV1645371.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHPDLPSKAAAMVRENVIAQLSNL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4663..5133
                     /gene="cynS"
                     /locus_tag="RHW33_001868"
     CDS             4663..5133
                     /gene="cynS"
                     /locus_tag="RHW33_001868"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV1645372.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARQVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5139..6320
                     /gene="cynX"
                     /locus_tag="RHW33_001869"
     CDS             5139..6320
                     /gene="cynX"
                     /locus_tag="RHW33_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV1645373.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSSRQTASSHRTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LALQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWVFHALCVVGLMIITLRFAPARFLQLWVKE
                     A"
     gene            complement(6423..7034)
                     /gene="lacA"
                     /locus_tag="RHW33_001870"
     CDS             complement(6423..7034)
                     /gene="lacA"
                     /locus_tag="RHW33_001870"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV1645374.1"
                     /translation="MNMPMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHYELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(7100..8353)
                     /gene="lacY"
                     /locus_tag="RHW33_001871"
     CDS             complement(7100..8353)
                     /gene="lacY"
                     /locus_tag="RHW33_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV1645375.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAILLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(8405..11479)
                     /gene="lacZ"
                     /locus_tag="RHW33_001872"
     CDS             complement(8405..11479)
                     /gene="lacZ"
                     /locus_tag="RHW33_001872"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV1645376.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAL
                     TNRPSQQLRSLNGEWQFVWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLGLNVENPKLWSAEIPNIYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTSATDIICPMYARVD
                     EDQLFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPMNVAPQGKQVIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISALQQWRLAENLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            complement(11602..12684)
                     /gene="lacI"
                     /locus_tag="RHW33_001873"
     CDS             complement(11602..12684)
                     /gene="lacI"
                     /locus_tag="RHW33_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV1645377.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAITVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPKT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            complement(12721..13674)
                     /locus_tag="RHW33_001874"
     CDS             complement(12721..13674)
                     /locus_tag="RHW33_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HDV1645378.1"
                     /translation="MRPLTVAIIAVAGFSPFHLSVPFIVFSEKMAEKKHFHVIICAEK
                     PGNVDSADGFSVTATHDYTAVIQADIVIIPYWGTITQKPPQKLLKALTTARDNGAQIV
                     GLCLGTFVLGYAGLLKNKRAATHWEFEREFQARFPQTHLDINALYVDDDGIITSAGTA
                     AALDCCLYIVRQHFGSDYANHIARRMVVPPYRTGGQAQFIEQPVPKNTHDERINLLLD
                     YLRQNIAQQHDLDSLAQRVMMSRRTLTRHFMKATGSSIAEWLITERLRRSQELLGSSQ
                     LPVERIAAEVGFLSPVTWRQHFKSHFGVSPAEWRKTFRGMA"
     gene            complement(13694..14470)
                     /locus_tag="RHW33_001875"
     CDS             complement(13694..14470)
                     /locus_tag="RHW33_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDV1645379.1"
                     /translation="MKITQIRNATQIIQYAGKTFLIDPLLAPKGSYPGFAGTARAEIR
                     NPTVELPCDLTTLLNVDALIVTHLHEDHWDETASRVVPKDKPIYVQNEQDAESLNKQG
                     FTHLTVLTDETKFGDITLHKTTGQHGSDRTYADPAMAKRLGAACGVVFQHPTEKTLYL
                     AGDTLWRDDVEVTMLTFQPEVVVLNAGFAHVLGHGAIIMGAEDILKTHFTLPEAQIVA
                     THMEAVNHCLLTRDALKEYARDNQIIEFVNVPEDGETLTF"
     gene            complement(14585..15532)
                     /gene="mhpR"
                     /locus_tag="RHW33_001876"
     CDS             complement(14585..15532)
                     /gene="mhpR"
                     /locus_tag="RHW33_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV1645380.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGACVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLASRPGDDYQLAR
                     EPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15609..17273
                     /locus_tag="RHW33_001877"
     CDS             15609..17273
                     /locus_tag="RHW33_001877"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV1645381.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            17275..18219
                     /gene="mhpB"
                     /locus_tag="RHW33_001878"
     CDS             17275..18219
                     /gene="mhpB"
                     /locus_tag="RHW33_001878"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV1645382.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            18237..19103
                     /gene="mhpC"
                     /locus_tag="RHW33_001879"
     CDS             18237..19103
                     /gene="mhpC"
                     /locus_tag="RHW33_001879"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV1645383.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADT
                     FNQLVLNFLARA"
     gene            19113..19922
                     /gene="mhpD"
                     /locus_tag="RHW33_001880"
     CDS             19113..19922
                     /gene="mhpD"
                     /locus_tag="RHW33_001880"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV1645384.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GQPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAAAFSSAAPKGSLS"
     gene            19919..20869
                     /gene="mhpF"
                     /locus_tag="RHW33_001881"
     CDS             19919..20869
                     /gene="mhpF"
                     /locus_tag="RHW33_001881"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV1645385.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVVRVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            20866..21879
                     /gene="dmpG"
                     /locus_tag="RHW33_001882"
     CDS             20866..21879
                     /gene="dmpG"
                     /locus_tag="RHW33_001882"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV1645386.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGADLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            22056..23267
                     /gene="mhpT"
                     /locus_tag="RHW33_001883"
     CDS             22056..23267
                     /gene="mhpT"
                     /locus_tag="RHW33_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV1645387.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQV
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLTSLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            23369..23908
                     /locus_tag="RHW33_001884"
     CDS             23369..23908
                     /locus_tag="RHW33_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV1645388.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(24033..24866)
                     /gene="fghA"
                     /locus_tag="RHW33_001885"
     CDS             complement(24033..24866)
                     /gene="fghA"
                     /locus_tag="RHW33_001885"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV1645389.1"
                     /translation="MELIEKHASFGGWQNVYRHYSASLKCEMNVGVYLPPKAAGEKLP
                     VLYWLSGLTCNEQNFITKSGVQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFSATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKEAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(24959..26068)
                     /gene="frmA"
                     /locus_tag="RHW33_001886"
     CDS             complement(24959..26068)
                     /gene="frmA"
                     /locus_tag="RHW33_001886"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV1645390.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            complement(26103..26378)
                     /gene="frmR"
                     /locus_tag="RHW33_001887"
     CDS             complement(26103..26378)
                     /gene="frmR"
                     /locus_tag="RHW33_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV1645391.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            complement(26538..27584)
                     /gene="fbpC"
                     /locus_tag="RHW33_001888"
     CDS             complement(26538..27584)
                     /gene="fbpC"
                     /locus_tag="RHW33_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDV1645392.1"
                     /translation="MSQKNFVELRNVTKRFGSNTVIDNINLTIPQGQMVTLLGPSGCG
                     KTTILRLVAGLEKPSEGQIFIDGEDVTHRSIQQRDICMVFQSYALFPHMSLGENVGYG
                     LKMLGVSRSEVKQRVKEALAMVDLEGFEDRYVDQISGGQQQRVALARALILKPKVLLF
                     DEPLSNLDANLRRSMRDKIRELQKQFNITSLYVTHDQSEAFAVSDTVLVMNKGHIMQI
                     GSPQDLYRQPASRFMASFMGDANLFPANFSEEYVDIYGYRLPRAAHFPAQGSGTVGVR
                     PEAITLSNHGEESQRCVIRHVAYMGPQYEVTVEWHGQEILLQVNATRLQPDIGEHYYL
                     EIHPYGMFVLADTA"
     gene            complement(27596..29674)
                     /locus_tag="RHW33_001889"
     CDS             complement(27596..29674)
                     /locus_tag="RHW33_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1645393.1"
                     /translation="MSQTLALHPVKKRDAIFLWVLLGWLAFILLPSWSLDYGLLESTS
                     DEILEAYGWSHFNISWLWYLLPSLLLVRPFREATREQRSRHYFDASWALLCMAFIVIS
                     ATVAGRGLGYAAIVLFVELGAIMTLALTRLEWLGGDRFVIGSLIAIVALIGIFIVWPS
                     IAIFIPMFTNDAGEFAPLAFMTVLSQAHIVQVIVNSIVLSIAVGIGCTFFGLVLAIYT
                     TRIAKRSAIIGRIFSILPIVTPPFVVGLGVTLMMGRSGYVTEFMVEWFGLTNTNWLYG
                     FTGIWLAQVLAFTPMAFMILDGAIKTIHPSLEEASYTLRASRWQTFNGVFVPLLKPAL
                     ANAFLIVVVQSLADFSNPLVLGGNFDVLATQIYFYITGSQLDYQAASTLGAFLLLFSL
                     LVFCIQYMWIGKRSYVTVSGKSYRGDVQPLPVTLVWSVVAILAIWIAFNALLYGSIFY
                     GSFTVNWGVDYTLTLDNFIKLFGQGMSDGAWPSLLDTLLYAGIAAPITAIFGLLIAWV
                     VVRQQFKGKKTIEFTTMLCFAVPGTVAGVSYILAFNSAPVYLTGTAAIVIISMVMRNV
                     PVGIRAGIAGLGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAIT
                     TVSAIVFLVTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFLFDWLIGEAR
                     ISRSKAKNQA"
     gene            complement(29743..30774)
                     /locus_tag="RHW33_001890"
     CDS             complement(29743..30774)
                     /locus_tag="RHW33_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1645394.1"
                     /translation="MKMTFTSTLIASAVALATLTGAAQAKGRLVVYCSATNEMCEAET
                     KAFGEKYDVKTSFIRNGSGSTLAKVDAEKKNPQADVWYGGTLDPQSQAGEMGLLQAYK
                     SPNLEQIMEKFRDPAKVKGNLSSAVYVGILGFGVNTDRLKEKNLPVPKCWKDLTKPEY
                     KGEIQIADPQSSGTAYTALATFAQLWGEDQAFDYLKQLNANVSQYTKSGIAPARNAAR
                     GETAIGIGFLHDYSLEKEQGAPLELISPCEGTGYEIGGVSILKGARNLDNAKLFVDWV
                     LSKEAQELAWKKGKSYQILTNTTAETSPNSLKLDDLKLINYDMDKYGSTDVRKALINK
                     WVSEVKMGK"
     gene            complement(30771..32087)
                     /gene="uhpC"
                     /locus_tag="RHW33_001891"
     CDS             complement(30771..32087)
                     /gene="uhpC"
                     /locus_tag="RHW33_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV1645395.1"
                     /translation="METTMHARSAREINQCYRTLRPRLLMCMIIGYAAFYLTRKSVNY
                     VLPALQTDLGLDKGDIGLLGSLFYLSYGLSKFTAGLWHDSHGQRGFMGVGLFATGLLN
                     VVFAFGESLTLLLVVWTLNGFFQGWGWPPCARLLTHWYSRNERGFWWGCWNMSINIGG
                     AIIPLISAFAAHWWGWQAAMLTPGIISMALGIWLTLQLKGTPQEEGLPTVGHWRHDPL
                     ELRQEQQSPPMGLWQMLRTTMLQNPLIWLLGVSYVLVYVIRIALNDWGNIWLTESHGV
                     NLLSANATVMLFEVGGLLGALFAGWGSDLLFSGQRAPMILLFTLGLMVSVAALWLAPV
                     HHYALLAVCFFTVGFFVFGPQMLIGLAAVECGHKAAAGSITGFLGLFAYLGAALAGWP
                     LSLVIERYGWPGMFSLLSVAAVLMGLLLMPLLMAGITTTHARRIKQ"
     gene            complement(32160..33701)
                     /locus_tag="RHW33_001892"
     CDS             complement(32160..33701)
                     /locus_tag="RHW33_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HDV1645396.1"
                     /translation="MSRNLRHWVISLFIVLAWGTGWLMLWTLSFYLTNNGQQAVLFLP
                     QGVYLALVILLSRRYWPALVLPPLLMMFWLHSEQLLNGYLMLATPVISLFPVLLAQNF
                     WHRFPLYWQRLTLLLATVTAASLLNTALLSPFMSGPIMLPGLTSFTGGVLLTPFVYLI
                     FEFLRQQHRYQLLGLDTHNPPLRTSLIIWCSLFFIIGIGTQMVLSPEIERLLLIVVFL
                     PNVVMAWKFGWQGGVLSGLLGSMMITIARQIGVGFSNLVELEIFLATQALLGIGLGIA
                     ISRQQHLALNLHHYRQRLEAELAARRALAEKLIHTEEDTRKNLARELHDEIGQNITAI
                     QIQSQLVKRARDPAQIQSAASQINELARRIHLSTRQLLRQLRPPALDELTFREALLHL
                     INEFAFSERGIHCQFAYQLNSTPENETVRFTLYRLLQELLNNICKHAEASEVTIILRQ
                     QGEVLHLEVSDNGVGIASGKMAGFGIQGMRERVSALGGDLTLEKQHGTRVIVNLPTFL
                     QQIAG"
     gene            complement(33701..34329)
                     /locus_tag="RHW33_001893"
                     /pseudo
     CDS             complement(33701..34329)
                     /locus_tag="RHW33_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            34632..35594
                     /gene="tauA"
                     /locus_tag="RHW33_001894"
     CDS             34632..35594
                     /gene="tauA"
                     /locus_tag="RHW33_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1645397.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTTELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            35607..36374
                     /gene="tauB"
                     /locus_tag="RHW33_001895"
     CDS             35607..36374
                     /gene="tauB"
                     /locus_tag="RHW33_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV1645398.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALTANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            36371..37198
                     /gene="tauC"
                     /locus_tag="RHW33_001896"
     CDS             36371..37198
                     /gene="tauC"
                     /locus_tag="RHW33_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV1645399.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            37195..38046
                     /gene="tauD"
                     /locus_tag="RHW33_001897"
     CDS             37195..38046
                     /gene="tauD"
                     /locus_tag="RHW33_001897"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV1645400.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLSFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(38153..39127)
                     /gene="hemB"
                     /locus_tag="RHW33_001898"
     CDS             complement(38153..39127)
                     /gene="hemB"
                     /locus_tag="RHW33_001898"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV1645401.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         9045 a        10369 c        10757 g         9072 t
ORIGIN      
        1 cgacaccatg cgccgatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaggcctg
       61 aaccgtaggt cggataaggc gctcgcgccg catccgacac cgtgcgcagt tgcctgatgc
      121 gacgctgacg cgtcttatta tgccgaccca ctgtttttac accgataatt tttcccccac
      181 ctttttgcac tcattcatat aaaaaatata tttccccacg aaaacgattg ctttttatct
      241 tcagatgaat agaatgcggc ggattttttg ggtttcaaac agcaaacgga ggaatttcgt
      301 gtcgcaagat aacaacttta gccaggggcc agtcccgcag tcggcgcgga aaggggtatt
      361 ggcattgacg ttcgtcatgc tgggattaac cttcttttcc gccagtatgt ggactggcgg
      421 cactcttgga accggtctta gctatcatga tttcttcctc gcagttctca tcggcaatct
      481 tctcctcggt atttacactt catttctcgg ttacattggc gcaaaaaccg gcctgaccac
      541 ccatcttctt gctcgcttct cgtttggtgt taaaggctca tggctgcctt cactgctgct
      601 gggcggcacc caggttggct ggtttggcgt tggcgtggcg atgtttgcca ttccggtggg
      661 taaggcaacc gggctggata ttaatttgct gattgccgtt tccggtttac tgatgaccgt
      721 caccgtcttc tttggcattt cggcgctgac agttctttcg gtgattgcgg ttccggcgat
      781 cgcctgtctt ggcggttatt ccgtgtggct ggctgttaac ggcatgggcg gcctggacgc
      841 cttaaaagcg gtcgttcccg cacaaccatt agatttcaat gtcgcgctgg cgctagttgt
      901 ggggtcattt atcagcgcgg gtacgctcac cgctgacttt gtccgctttg gtcgcaatgc
      961 caaactggcg gtgctggtgg cgatggtggc ctttttcctc ggcaactcgt tgatgtttat
     1021 tttcggtgca gcgggcgctg cggcactggg gatggcagat atctctgatg tgatgattgc
     1081 tcagggcctg ctgctgcctg cgattgtggt gctggggcta aatatctgga ccaccaacga
     1141 taacgcgctc tatgcgtcgg gtttaggttt cgccaacatt accgggatgt cgagcaaaac
     1201 cctttcggta atcaacggta ttatcggtac ggtctgtgca ttatggctgt ataacaattt
     1261 tgtcggctgg ttgaccttcc tttcggcagc tattcctccg gtgggtggcg tgatcattgc
     1321 cgactatctg atgaaccgtc gccgctatga gcactttgcg accacgcgta tgatgagtgt
     1381 caattgggtg gcgattctgg cggtcgcctt ggggattgct gcaggccact ggttaccagg
     1441 aattgttccg gtcaacgcgg tattaggtgg cgcgctgagc tatctgatcc ttaacccgat
     1501 tttgaatcgt aaaacgacag cagcaatgac gcatgtggag gctaacagtg tcgaataacg
     1561 ctttacaaac aattattaac gcccggttac caggcaaaga ggggctgtgg cagattcatc
     1621 tgcaggacgg aaaaatcagc gccattgatg cgcaatccgg cgtgatgccc ataactgaaa
     1681 acagcctgga tgccgaacaa ggtttagtta taccgccgtt tgtggagcca catattcacc
     1741 tggacaccac gcaaaccgcc ggacaaccga actggaatca gtccggcacg ctgtttgaag
     1801 gcattgaacg ctgggccgag cgcaaagcgt tattaaccca tgacgatgtg aaacaacgcg
     1861 catggcaaac gctgaaatgg cagattgcca acggcattca gcatgtgcgt acccatgtcg
     1921 atgtttcgga tgcaacgcta actgcgctga aagcaatgct ggaagtgaag caggaagtcg
     1981 cgccgtggat tgatctgcaa atcgtcgcct tccctcagga agggattttg tcgtatccca
     2041 acggtgaagc gttgctggaa gaggcgttac gcttaggggc agacgtagtg ggggcgattc
     2101 cgcattttga atttacccgt gaatacggcg tggagtcgct gcataaaacc ttcgccctgg
     2161 cgcaaaaata cgaccgtctg atcgacgttc actgtgatga gatcgatgac gaacagtcgc
     2221 gctttgtcga aaccgttgct gccctggcgc accgtgaagg catgggcgcg cgagttaccg
     2281 ccagccacac cacggcaatg cactcctata acggggcgta tacctcacgc ctgttccgct
     2341 tgctgaaaat gtccggtatt aactttgtcg ccaacccgtt ggtcaatatt catctgcaag
     2401 ggcgtttcga tacgtatcca aaacgtcgcg gcatcacgcg cgttaaagag atgctggagt
     2461 ccggcattaa cgtctgcttt ggtcacgatg atgtcttcga tccgtggtat ccgctgggaa
     2521 cggcgaatat gctgcaagtg ctgcatatgg ggctgcatgt ttgtcagttg atgggctacg
     2581 ggcaaattaa cgatggcctg aatttaatca cccaccacag cgccaggacg ttgaatttgc
     2641 aggattacgg cattgccgcc ggaaacagcg ccaacctgat tatcctgccg gctgaaaatg
     2701 gatttgatgc gctgcgccgt caggttccgg tacgttattc tgtacgtggc ggcaaggtga
     2761 ttgccagcac acaaccggca caaaccaccg tatatctgga gcagccagaa gccatcgatt
     2821 acaaacggtg atcggctggg ctgtgtgttg acaaagcgag cgtagtttat gccggatgcg
     2881 gcgtgaacgc cttatccggc ctacgtagag cactgaactc gtaggcctga taagcgtagc
     2941 gcatcaggca attgtgtcag tggttaccgc gattcattcc cgccaacaac cgcgcattta
     3001 tccaacgcca tgtgcaaaaa tgccttcgcg gcggctgtct gccagctatt tttccgccgc
     3061 aacaaaaccg ccgttctctc cagtagtggc ggggcaagag aaatagcttt aagcccgtca
     3121 tgttgtgtgg caatcgctgc tggtaacaat gtggaaaggg aagtgcggcg aatcagctcc
     3181 agaaccgcgc taattgagtt cgcctcaatg accacctgtg gatgcagccc cgctttctcg
     3241 cagtagtggt caatttgctc tctggtggca aattccgcgc tgagcaggac cagtttttca
     3301 tcatgcaagc gactcaacgc catctgttca tgggcggcca gcggatgatg ttgcgccacg
     3361 actaacgcta aactttctgt cagtaaagga attgcctcca gctccggcga atgcacaggc
     3421 gcgaaggcaa tcccaacgtc caactcgtcg cggcaaagca gatcttcgat tttctcctgc
     3481 gacatttcct gtagctggag cgtgatgccg gggtagcgcg catagaaatc cgccattaag
     3541 gggccgataa agtagctcgt aaaggtgggg gtgacggcga tacgcagcga tcctctcgtc
     3601 aaatcggcaa catcatgaat cgcccgttta cccgctccca gttcctgtaa cgcccggctg
     3661 gcgtactgtc gccagacttc tcctgcatca gtgagacgaa tcgttcgccc gctacggtca
     3721 aacagcggca cgcctaaact ctcctctaac tggcgaatct gctgggaaag cgcaggctga
     3781 gagacgtgca acgcactggc ggcacgggtg aagctgccat gttcagccac ggcaagaaaa
     3841 taattgatat gtcgagagag cattcgccaa cctataagta aatccaatag aactcatcat
     3901 aaatgagact tttaccttat gacaatcggc gagtaatctg cctctcattc cagagacaga
     3961 cagaggttaa cggtgaaaga gattattgat ggattcctta aattccagcg cgaggcattt
     4021 ccgaagcggg aagccttgtt taaacagctg gcgacacagc aaagcccgcg cacccttttt
     4081 atctcctgct ccgacagccg tctggtccct gagctggtga cgcaacgtga gcctggcgat
     4141 ctgttcgtta ttcgcaacgc gggcaatatc gtcccttcct acgggccgga acccggtggc
     4201 gtttctgctt cggtggagta tgccgtcgct gcgcttcggg tatctgacat tgtgatttgt
     4261 ggtcattcca actgtggcgc aatgaccgcc attgccagct gtcagtgcat ggaccatatg
     4321 cctgccgtct cccactggct gcgttatgcc gattcagccc gcgtcgttaa tgaggcgcgc
     4381 ccgcatcccg atttaccgtc aaaagctgcg gcgatggtac gtgaaaacgt cattgctcag
     4441 ttgtctaatt tgcaaactca tccatcggtg cgcctggcgc tcgaagaggg gcggatcgcc
     4501 ctgcacggct gggtctacga cattgaaagc ggcagcatcg cagcgtttga cggcgcaacc
     4561 cgccagtttg tgccactggc cgctaatcct cgcgtttgtg ccataccgct acgccaaccg
     4621 accgcagcgt aaccttattt ttaaaccatc aggagatgca ccatgattca gtcgcaaatt
     4681 aatcgcaata ttcgtcttga tcttgccgat gccattttgc tcagcaaagc taaaaaagat
     4741 ctctcatttg ccgagattgc cgacggcacc ggtctggcag aagcctttgt gaccgcggct
     4801 ttgctgggtc agcaggcgct tcctgccgac gccgcccgcc aggtcggggc gaagctggat
     4861 ctcgacgaag acgccattct actgttacag atgatcccac tgcgtggctg cattgatgac
     4921 cgtattccaa ccgacccaac gatgtatcgt ttctatgaaa tgttgcaggt gtacggcaca
     4981 accctgaaag cgttggttca tgagaagttt ggcgatggca ttatcagcgc gattaacttc
     5041 aaactcgacg ttaagaaggt ggcggacccg gaaggtggcg aacgtgcggt catcacctta
     5101 gatggtaaat atctgccgac caaaccgttc tgacagccat gcgcaaccat caaaagacgt
     5161 tcacgatgct gctggttctg gtgctgattg gtcttaatat gcgaccactg ctcacctccg
     5221 tcgggccact gctaccgcaa ttgcgccagg caagcggaat gagctttagc gtggctgccc
     5281 tgttgaccgc tctgccggtg gttaccatgg gcgggctggc gctggccgga agctggcttc
     5341 atcagcatgt cagcgaacgt cgcagcgtcg ccatcagttt gttactgatt gccgtcggtg
     5401 cattgatgcg tgagctttac ccccaaagtg cgctgctgct tagcagcgca ctgctcggtg
     5461 gggtggggat cggcatcatt caggcagtga tgccttcggt gattaaacgg cggtttcagc
     5521 agcgcacgcc actggtgatg gggctgtggt ccgcggctct gatgggcggc ggtggacttg
     5581 gtgccgccct gacgccctgg ttagttcaac atagcgaaac ctggtatcaa acactcgcct
     5641 ggtgggcgct gcctgccgtt gttgcgctct ttgcctggtg gtggcaaagc tcccgccaga
     5701 ccgcttcttc ccacaggaca acaaccactc cggttcgcgt tgtattcact ccccgcgcgt
     5761 ggacgctggg tgtttacttc ggtctgatta acggcggtta cgccagcctg attgcctggt
     5821 tacccgcctt ctatattgag atcggtgcca gcgcgcagta cagcggttcc ttgctggcat
     5881 tgatgacgct tgggcaagcc gcaggagctt tgctgatgcc tgctatggct cgccatcagg
     5941 atcggcgcaa acttttgatg ctggcgctgg cgttacaact ggtgggtttc tgcggcttta
     6001 tctggctgcc gctacaatta ccagtattgt gggcgatggt gtgtggttta ggtctgggcg
     6061 gcgcgtttcc gctctgtttg ctgctggcgc tcgaccactc tgtgcaaccg gctattgctg
     6121 gcaagctggt ggcgtttatg caggggatcg gttttatcat cgccgggctt gccccgtggt
     6181 tttctggcgt gctgcgtagt atcagcggca attacctgat ggactgggtg tttcatgcgc
     6241 tgtgcgtcgt tgggctgatg attataaccc tgcgttttgc accagcacgt tttctgcagc
     6301 tgtgggtcaa agaggcatga tgcgacgctt gtttctgcgc tttgttcacg ccggatgcgg
     6361 cctacgtaga tcactgaatt tgtaggcctg ataagcgaag cgtatcaggc aatttttata
     6421 atttaaactg acgattcaac tttgtaatct ctgaaataat attgcttatc ccggtcgttt
     6481 atttcgcgaa taacccggca aggaacgcca gcagccacga cgtttggtgg aatgtctttt
     6541 atgacgacac tacccgcgcc aataacagaa ttatccccga tggtgacgcc tggattaata
     6601 accacatgac ttccgatcca gacgttattg ccaatcgtta tcggaaaaga gtacatctca
     6661 ccgttttttc tcaattcata gtgtacaggg tgtcccgtaa cggaaagagt aacattgggt
     6721 gcaatcaata cgttatcacc gatcgttacc gtgtagtcgt cgacaatggt taaattgaaa
     6781 tttgcataaa aattgcggcc tatatggatg ttggaaccgt aagagaaata gacaggtggt
     6841 tctatccagg cgttttcccc taccgtggca aacatttctt taatcaggct ttctcttttt
     6901 tcaacttctg atggatgcga gtgattaaac tcatacatta acgttttccc acgaagtctc
     6961 ttttccggta agccttcgca catatcggta aatagcttgc ctgcttttat tctttcggtc
     7021 attggcatgt tcattgcgat cattccgtta tgatatgttg gtcggataag gcgctcgcgc
     7081 cgcatccgac attgattgct taagcgactt cattcacctg acgacgcagc agggaaagcg
     7141 ggccggggcc gctaagcgtg aacacggaaa ttaaggtgaa gcccagcgcc accagaccca
     7201 gcaccagata agcgccctgg aaaccgatgc tttcatacat attgcccgcc agtacagaca
     7261 taaaaatcat cgccagttgc ttaaagaagc agaaacagac cagataaatc gtcgctgaaa
     7321 aacgcacttc aaactggctg gtaatatatt taaaacagcc caccagcagg aacggtactt
     7381 caaacatatg cagcgttttc agaataacta cttccagcgc tgaggtggcg aacgatgagc
     7441 caataatacg tacagacata atagtgccag ccagcagcag ggcgtttttc ccaccgatgc
     7501 gattaatgat cagtggcgca aagaacataa tcgaggcgtt aagtaattcg cccattgtcg
     7561 ttacgtagcc aaatacccgc gtcccctgtt caccggtagc aaagaacgaa gtaaagaaat
     7621 tagcaaactg ttggtcaaaa acatcgtagg tgcaggaaac gccaataaca tacagtgaca
     7681 aaaaccacag ttttggctgt ctgaacagtt ccagcgccag tttaaggcta aatgccgaat
     7741 ggttggcacc taccgcattg gcaaccgtgg cggaagaggg cgcatccgtt ttggcgaaaa
     7801 agagtaaaat ggcgaggatg agtgcacacc cagaacccag ccagaaaacg aactgattat
     7861 tgatggtgaa catgatgccg acaattgagg cacacagcgc ccagccaaca cagccaaaca
     7921 tccgcgcgcg accaaattcg aaattactgc gacggctgac tttctcgata aatgcctcta
     7981 ctgcgggcgc accggcgtta aaacaaaagc caagataaat accaccaaca atcgatccta
     8041 ctaaaatgtt gtattgtaac agtggcccga agataaaaat aaagaacggc gcaaacatca
     8101 ctaacatgcc ggtaataatc cacagcaggt atttgcgcag cccgagtttg tcagaaagca
     8161 gaccaaatag cggttggaat aatagcgaga acagagaaat agcggcaaaa ataatacccg
     8221 tatcactttt gctgatatgg ttgatgtcat gtagccaaat cgggaaaaac gggaagtagg
     8281 ctcccatgat aaaaaagtaa aagaaaaaga ataaaccgaa catccaaaag tttgtgtttt
     8341 ttaaatagta cataatggat ttccttacgc gaaatacggg cagacatggc ctgcccggtt
     8401 attattattt ttgacaccag accaactggt aatggtagcg accggcgctc agctggaatt
     8461 ccgccgacac tgacgggctc caggagtcgt cgccaccaat ccccatatgg aaaccgtcga
     8521 tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt tccatcagtt
     8581 gttgttggct gtagcggctg atgttgaact ggaagtcgcc gcgccactgg tgtgggccat
     8641 aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg tacggggtat
     8701 acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg cggtcgggat
     8761 agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc gccagctggc
     8821 aggtcaggcc aatccgtgcc ggatgcggtg tatcgctcgc tacttcaaca tcaacggtaa
     8881 tcgccatttg accactacca tcaatccggt aggttttccg gctaataaat aaggtttttc
     8941 cctgatgctg ccatgcgtgg acagtggtaa tcagcaccgc gtcggcaagt gtatctgccg
     9001 tgcactgcaa caacgccgct tcggcctggt aatggcccgc cgccttccag cgttcgaccc
     9061 aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc agcggtgcac
     9121 gggtgaactg atcgcgcagc ggcgtcagca gttgtttttc atcgccaatc cacatctgtg
     9181 aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt atccagctcg atgcaaaaat
     9241 ccgtttcgct ggtggtcagt tgcgggatgg cgtggggcgc ggaggggagt gtcacgctga
     9301 ggttttccgc cagacgccac tgctgcaagg cgctgatgtg tccggcttct gaccatgcgg
     9361 ttgcgttcgg ttgcactacg tgaaccgtta gccagagttg tccggcgctc tccggctgcg
     9421 gtagttcagg cagttcaatc acctgtttac cttgtggagc gacattcata ggtacttcac
     9481 cgcttgccag cggcttgcca tccagcgcca ccatccagtg caggagctcg ttatcgctat
     9541 gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg aactggaaaa
     9601 actgctgctg gtgttttgct tccgtcagcg ccggatgcgg cgtgcggtcg gcaaagacca
     9661 gaccgttcat gcagaactgg cgatcgtttg gcgtatcgcc aaaatcaccg ccgtaagccg
     9721 accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag tcccagacga
     9781 agccgccctg taaacggggg tactgacgaa acgcctgcca gtatttagcg aagccgccaa
     9841 gactgttacc cattgcgtgg gcatattcgc aaaggatcag cgggcgcgtc tctccaggca
     9901 acgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagagctgg tcttcatcca
     9961 cgcgcgcgta catcgggcaa ataatatcgg ttgcggaggt gtcggctccg ccgccttcat
    10021 actgtaccgg gcgggaagga tcgacagatt tgatccagcg atagagtgcg tcgtgattag
    10081 cgccgtggcc tgactcattt cccagcgacc agataatcac actcgggtga ttacgatcgc
    10141 gctgcaccat tcgcgttacg cgttcgctca tcgcgggtag ccagcgcgga tcatcggtca
    10201 gacgattcat tggcaccatg ccgtgggttt caatgttggc ttcatccacc acatacaggc
    10261 cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa cagcgcacgg
    10321 cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc tcatccatga
    10381 cctgaccatg cagaggatga tgctcgtgac ggttaacgcc gcgaatcagc aacggcttgc
    10441 cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca tcgcaggctt
    10501 ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga tagatattcg
    10561 ggatttcggc gctccacagt ttcgggtttt cgacgttcag acctagtgtg acgcgatcgg
    10621 cataaccgcc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg ctggcgacct
    10681 gcgtttcacc ctgccacaaa gaaaccgtca cccgcagctc atcgcgcagc tcgccgtaca
    10741 tctgaacttc tgcctccagt accgcgcggc tgaaatcatc attaaagcga gtggcaacat
    10801 ggaaatcgct gatttgtgtg gtcggtttgt gcagcaacga gacgtcacgg aaaatgccgc
    10861 tcatccgcca catatcctga tcttccagat aactgccgtc actccagcgc agcaccatca
    10921 ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca gacggcaaac
    10981 gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac gccgagttaa
    11041 cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca acattaaatg
    11101 tgagcgagta acaacccgtc ggattctccg tgggaacaaa tggcggattg accgtaatgg
    11161 gataggtcac gttggtgtag atgggcgcgt cgtaaccgtg catctgccag tttgagggga
    11221 cgacgacagt atcggcgaca ggaagatcgc actccagcca gctttccggc accgcttctg
    11281 gtgccggaaa ccagacaaat tgccactcac cattcaggct gcgcaactgt tgggaagggc
    11341 gattggtgag ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
    11401 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaatacg acggccagtg
    11461 aatccgtaat catagtcata gctgtatcct gtgtgaaatt gttatccgct cacaatttca
    11521 cacaacatac gagtcggacg cataaagtgt aaagcctggg gtgcctaatg agtgagctga
    11581 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaactt gtcgtgccag
    11641 ctgcattaat gaatcggcca acgcgcgggg agaagcggtt tgcgtcttgg gcgccagggt
    11701 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
    11761 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
    11821 ggtggttaac ggcgggatat aacacgagct gtcttcggta tcgtcgtatc ccactaccga
    11881 gatatccgca ccaacgcgca acccggactc ggtaatggcg cgcattgcgc ccagcgccat
    11941 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcatttagca tttgcatggt
    12001 ttgttgaaaa ccggacatgg cactccagtc gccctcccgt tccgctatcg gctgaatttg
    12061 attgcgagtg agatatttat gccagcccgc cagacgcaga cgcgccgaga cagaacttaa
    12121 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
    12181 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggagtctggt cagagacatc
    12241 aagaaataac gccggaacat tagcgcaggc agcttccaca gtaatggcat cctggtcatc
    12301 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
    12361 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
    12421 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
    12481 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
    12541 tatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
    12601 gctggcctga ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
    12661 atcgtatagc gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
    12721 tcatgccata ccgcgaaagg ttttgcgcca ttcggcgggg ctgacgccga agtgagattt
    12781 aaaatgctga cgccaggtca caggtgagag aaaacccacc tcagccgcta tccgctcaac
    12841 gggcaactga ctggatccca acagttcctg gctacggcgt aagcgttcag taatgagcca
    12901 ttcggcgata ctcgaaccgg tcgctttcat aaaatggcga gttaatgtgc ggcgactcat
    12961 cattactcgc tgcgccagcg agtcgagatc atgctgttgc gcaatgtttt gccgcaggta
    13021 atccagcagg aggtttatgc gttcatcatg ggtatttttc ggcaccggtt gctcaataaa
    13081 ctgcgcctga ccgccggtgc gatatggcgg tacgaccatc cgtcgggcaa tatggttagc
    13141 atagtcgctg ccaaaatgtt gccgaacaat atacaaacag caatccagcg ccgcggcagt
    13201 accggcagag gtaataatgc cgtcatcgtc tacgtacaac gcgttaatat ccagatgtgt
    13261 ttgtggaaaa cgtgcctgaa attcacgctc gaactcccag tgcgtggcgg cacgcttatt
    13321 tttcagtaaa cctgcatagc cgagcacaaa cgtgcccagg caaagcccga caatctgtgc
    13381 gccgttatcc cgtgcggtcg ttaaggcttt cagcagtttt tgtggcggtt tttgtgtaat
    13441 tgttccccag taaggaatta tcacaatatc cgcctggatg actgcggtat agtcatgggt
    13501 ggcggttacg gaaaacccat cggcagagtc cacgtttccc ggcttttcag cgcagataat
    13561 tacgtgaaag tgttttttct ccgccatttt ttcgctaaat acgataaacg gtacggaaag
    13621 gtgaaaagga ctaaaaccgg caacggcgat aatggcaaca gttagcgggc gcataacact
    13681 ctttatctcc gaattagaat gtcaacgttt caccatcttc tggtacgttc acaaattcga
    13741 taatttgatt atctctggcg tattccttga gcgcatcacg cgtcagcaaa cagtggttta
    13801 cggcctccat atgtgtcgcg acaatttgcg cttctggcag ggtaaaatgt gttttgagaa
    13861 tatcttctgc tcccataatt atcgcgccat gccccagaac atgcgcaaat ccggcattca
    13921 acacaaccac ttctggttgg aaagttagca ttgtaacctc gacgtcgtca cgccagaggg
    13981 tatcgccagc gagatagagc gttttctctg tcgggtgctg gaaaacaacg ccgcaggctg
    14041 cgcctaaacg cttcgccata gccgggtcgg cataggtgcg atcggagccg tgctgccctg
    14101 tcgttttatg cagagtaata tcgccaaatt tagtctcgtc ggtaagtacg gtaagatgcg
    14161 taaatccttg tttattcagt gattcagcat cttgctcatt ttgcacataa atcggtttat
    14221 ctttagggac gacgcgggag gctgtttcgt cccagtgatc ttcatgcaaa tgggttacga
    14281 ttagggcgtc aacgttgagt aacgttgtta aatcgcacgg taattctacc gttggattgc
    14341 ggatttctgc gcgtgccgtt ccggcaaaac ccggataact tcctttaggc gcgagtaacg
    14401 ggtcgataag aaatgttttg ccagcgtatt ggataatttg ggtggcatta cgaatttgcg
    14461 taattttcat gatttgctcc acatcacagt attgatgtca ttagcgtagc gtgcagcccg
    14521 tactgtgcat gtgggcaaat gggcgaataa cgatgagaat gggccaaatt acacattggc
    14581 gtaactagcg taaatgcacg ccgcttcgcc gtccggctac cagaatagcc tgtgattcaa
    14641 ccccttcttc gatctgtttt gctacccgtt gtagcgccgg aagatgcttt tccgctgcct
    14701 gttcgatggt cattgcgctc gccatataca ccagattcag acagccaatc acccgttgtt
    14761 cactgcgcag cggtacggcg atagaggcga tcttctcctc ctgatcccag ccgcggtagt
    14821 tctgtccgta accctctttg cgcgcgcgcg ccagaatggc ttccagcttt aacggttctc
    14881 gtgccagttg atagtcatca ccggggcggg aggctaacat ttcgattaat tccttgcgtt
    14941 cttgttccgg gcaaaaggcc agccaggtca ggcccgaggc ggttttcaga agcggcaaac
    15001 gtcgcccgac cattgcccgg tgaaaggata agcggctgaa acggtgggtg gtttcgcgta
    15061 ccaccattgc atcaacatcc agcgtggaca catctgtcgg ccataccact tcgcgcaaca
    15121 gatcgcccag cagtggggcc gccagtgcag aaatccactg ttcgtcacga aatccttcgc
    15181 ttaattgccg cactttgatg gtcagacgaa aactatcatc ggagggacta cgccggacat
    15241 atccctcttc ctgcagcgtc tccagcagtc gccgcacagt ggtgcgatgc aggccgctga
    15301 gttccgccag tagcccgacg caggcaccgc cgtcaagttt atttaacata tttaataaca
    15361 ttagaccgcg ggttaaaccg cgcacggttt tgtattccgt ctgctcattg ttctgcatat
    15421 taattgacat ttctatagtt aaaacaacgt tgtgcacctg gtgcacattc gggcatgttt
    15481 tgattgtagc cgaaaacacc cttcctatac tgagcgcaca ataaaaaatc atttacatgt
    15541 ttttaacaaa ataagttgcg ctgtactgtg cgcgcaacgg cattttgtcc gagtcgtgag
    15601 gtactgaaat ggcaatacaa caccctgaca tccagcctgc tgttaaccat agcgttcagg
    15661 tggcgatcgc tggtgccggt ccggtcgggc tgatgatggc gaactatctc ggccagatgg
    15721 gcattgacgt gctggtggtg gagaaactcg ataagttgat cgactacccg cgtgcgattg
    15781 gtattgatga cgaggcgctg cgcaccatgc agtcggtcgg cctggtcgat aatgttctgc
    15841 cgcatactac gccgtggcac gcgatgcgtt ttctcacccc gaagggccgc tgttttgctg
    15901 atattcagcc aatgaccgat gaatttggct ggccgcgccg taacgccttt attcagccgc
    15961 aggtcgatgc ggtgatgctg gaaggggtgt cgcgttttcc gaatgtgcgc tgcttgtttt
    16021 cccgcgagct ggaggccttc agtcagcaag atgacgaagt gaccttgcac ctgaaaacgg
    16081 cagaagggca gcgggaaata gtcaaagccc agtggctggt ggcttgtgat ggtggggcaa
    16141 gttttgtccg tcgcaccctg aatgtgccgt ttgaaggtaa aactgcgcca aatcagtgga
    16201 ttgtggtaga tatcgccaac gatccgttaa gtacgccgca tatctatttg tgttgcgatc
    16261 cggtgcgccc gtatgtttct gccgcgctac ctcatgcggt acgtcgcttt gaatttatgg
    16321 tgatgccagg agaaaccgaa gaacagctgc gtgagccgca aaatatgcgc aagctgttaa
    16381 gcaaagtgct gcctaatccg gacaatgttg aattgattcg ccagcgtgtc tacacccaca
    16441 acgcgcgact ggcgcaacgt ttccgtattg atcgcgtact gctggcgggc gatgccgcgc
    16501 acatcatgcc ggtatggcag gggcagggct ataacagtgg tatgcgcgac gcctttaacc
    16561 tcgcatggaa actggcgttg gttatccagg ggaaagcccg cgatgcgctg ctcgatacct
    16621 atcaacaaga acgtcgcgat cacgccaaag cgatgattga cctgtccgtg acggcgggca
    16681 acgtgctggc tccgccgaaa cgctggcagg gtacgttacg tgacggcgtt tcctggctgc
    16741 tgaattatct gccgccagta aaacgctact tcctcgaaat gcgcttcaag ccgatgccgc
    16801 aatattacgg cggtgcgctg gtgcgtgagg gcgaggcgaa gcactctccg gtcggcaaga
    16861 tgtttattca gccgaaagtc acgctggaaa acggcgacgt gacgctactc gataacgcga
    16921 tcggcgcgaa cttcgcggta attggctggg gatgcaatcc gctgtggggg atgagcgacg
    16981 agcaaatcca gcagtggcgc gcgttgggca cacgcttcat tcaggtggtg ccggaagtgc
    17041 aaattcatac cgcacaggat aaccacgacg gcgtactacg cgtgggggat acgcaaggtc
    17101 gcctgcgtag ctggttcgcg caacacaatg cttcgctggt ggtgatgcgc ccggatcgct
    17161 ttgttgccgc caccgccatt ccgcaaaccc tgggcaagac cctgaataaa ctggcgtcgg
    17221 tgatgacgct gacccgccct gatgccgacg tttctgtcga aaaggtagcc tgatatgcac
    17281 gcttatcttc attgtctttc ccactcgccg ctggtggggt atgtcgaccc ggcacaagag
    17341 gtactcgatg aggtcaatgg cgtgattgcc agcgcccgcg agcgtattgc ggctttctcc
    17401 cctgaactgg tggtgctgtt tgcgccagat cactacaacg gctttttcta tgacgtgatg
    17461 ccaccgttct gtttaggcgt tggagcgacg gcaattggtg atttcggcag tgcggcagga
    17521 gagctgccag tgcctgtgga gctggcggag gcctgtgcgc atgccgtcat gaagagcggg
    17581 atcgatcttg ccgtttctta ctgtatgcag gtggaccacg ggttcgccca gccgctggag
    17641 ttcctgctcg gtgggctgga taaggtgcca gttctgcctg tgttcatcaa cggtgtcgcc
    17701 acgccgctgc ccggtttcca gcgtacccgc atgttgggtg aagccattgg acgtttcacc
    17761 agcactctca ataaacgcgt gctgttcctg ggttccggtg ggctttccca tcagccgccg
    17821 gtgcccgaac tggcgaaagc cgatgcccat atgcgcgacc gtctgttggg gagcgggaaa
    17881 gatttacccg ccagtgagcg cgaattgcgt cagcaacggg tgattagcgc cgctgagaag
    17941 tttgttgagg atcagagaac tctgcatccg ctcaacccga tttgggataa ccagttcatg
    18001 actttgctgg agcagggacg catacaggaa ctggatgccg tcagtaacga agagctttcc
    18061 gccattgccg gaaagtcgac acatgaaatc aaaacctggg tcgccgcttt tgccgctatt
    18121 tctgcatttg gcaactggcg tagcgaaggg cgttattacc gcccaatccc ggagtggatt
    18181 gccggatttg gctcgttaag cgccagaaca gagaactgaa tatgcaggag aagatgatga
    18241 gttatcagcc acaaaccgaa gccgccacca gccgttttct gaatgtagaa gaagcgggta
    18301 aaacgctgcg catccatttt aatgactgcg gacaaggcga cgaaaccgtt gtcctgctgc
    18361 atggttccgg cccgggtgct actggctggg cgaacttcag ccgcaatatc gatccgctgg
    18421 tagaggcggg ctatcgggtg atcctgctgg attgtccggg ttggggcaag agcgattcga
    18481 tcgttaatag tggttcgcga tcggatctta atgcacgaat cctgaaaagc gtggtggatc
    18541 aactggatat cgccaaaatc cacctgctgg gcaactcgat gggtggccat agttctgtgg
    18601 cgttcaccct taactggccg gagcgcgtcg gcaaactggt gctgatgggc ggcggtacgg
    18661 gcggcatgag tttgtttacg ccgatgccaa ccgaaggtat taagcgactg aatcagcttt
    18721 atcgtcagcc gactatcgaa aacctgaagc tgatgatgga tatcttcgtt tttgatacca
    18781 gcgatttgac cgacgccctg tttgaagcgc gcctgaataa tatgctgtcg cgccgcgatc
    18841 acctggaaaa ctttgttaag agcctggaag ccaatccgaa acagttcccg gattttggcc
    18901 cacgtctggc ggaaatcaaa gcgcaaaccc tgattgtctg ggggcgcaac gaccgctttg
    18961 tgccgatgga tgcggggctg cgtctactgt ccggcattgc tggttctgaa ctgcatatct
    19021 tccgcgactg tggtcactgg gcgcagtggg aacatgccga cactttcaat caactggtgc
    19081 tgaatttcct cgcacgtgct taaggaacgg tcatgacgaa gcatactctt gagcaactgg
    19141 cggcggattt acgccgcgcc gcagagcagg gcgaagcgat tgcgccgctg cgcgatctga
    19201 ttggtatcga taacgctgaa gcggcctacg ccattcagca cataaatgtg caatatgacg
    19261 ttgcgcaggg gcgtcgcgtg gtagggcgta aagtgggcct gacacatccg aaagtgcagc
    19321 aacaactggg cgttgatcaa ccggatttcg ggacattatt tgccgacatg tgttatggcg
    19381 ataatgaaat cattcctttt tcccgtgttc tgcaaccccg cattgaagcg gagatcgcac
    19441 tggtgttgaa ccgcgatttg cccgcaaccg atatcacctt cgacgaattg tataacgcca
    19501 ttgaatgggt acttccggcg ctggaggtgg tgggtagccg cattcgcgac tggtcgattc
    19561 agtttgtcga taccgtggca gataacgctt cctgcggggt gtatgtcatc ggcggtccgg
    19621 cgcaacgtcc ggcggggtta gacctgaaaa actgcgccat gaagatgacg cgtaataacg
    19681 aagaggtttc cagcgggcgc ggcagcgaat gcctgggaca tccgcttaat gccgccgtct
    19741 ggctggcacg caaaatggcc agtctgggtc aaccgctgcg cgctggagat atcattctta
    19801 ccggggcatt aggcccgatg gtggcggtga atgcgggcga tcgttttgaa gcccatattg
    19861 aaggcatagg ttcagttgct gcggcatttt caagcgcagc cccaaaagga agtctgtcat
    19921 gagtaagcgt aaagtcgcca ttatcggttc tggcaacatt ggcaccgatc tgatgattaa
    19981 aattttgcgt cacggtcagc atctggagat ggcggtgatg gtcggcattg atcctcagtc
    20041 cgacggtctg gcgcgtgcca gacgtatggg cgtcgccacc acccatgaag gggtgatcgg
    20101 gctgatgaac atgcctgaat ttgctgatat cgacattgta tttgatgcga ccagcgccgg
    20161 tgctcatgtg aaaaacgatg ccgctttgcg cgaagcgaaa ccggatattc gcttaattga
    20221 cctgacgcct gctgccatcg gcccttactg cgtgccggtg gttaacctcg aggagaacgt
    20281 cgatcaactg aacgtcaaca tggtcacctg cggcggccag gccaccattc caatggtggc
    20341 ggcagtttca cgcgtagtgc gtgttcatta cgccgaaatt atcgcctcta tcgccagtaa
    20401 atctgccgga cctggcacgc gtgccaatat cgatgaattt acggaaacca cttcccgagc
    20461 cattgaagtg gtgggcggcg cggcaaaagg gaaggcaatt atcgtgctta acccagcaga
    20521 gccaccgttg atgatgcgtg acacggtgta tgtattgagc gacgaagctt cacaagatga
    20581 tatcgaagcc tcaatcaatg aaatggctga ggcggtgcag gcttacgtac cgggatatcg
    20641 cctgaaacag cgcgtgcagt ttgaagttat cccgcaggat aaaccggtca atttaccggg
    20701 cgtggggcaa ttctccggac tgaaaacagc ggtctggctg gaagtcgaag gcgcagcgca
    20761 ttatctgcct gcctatgcgg gcaacctcga cattatgact tccagtgcgc tggcgacagc
    20821 ggaaaaaatg gcccagtcac tggcgcgcaa ggcaggagaa gcggcatgaa cggtaaaaaa
    20881 ctttatatct cggacgtcac gttgcgtgac ggtatgcacg ccattcgtca tcagtattcg
    20941 ctggaaaacg ttcgccagat tgccaaagca ctggacgatg cccgcgtgga ttcgattgaa
    21001 gtggcccacg gcgacggttt gcagggttcc agctttaact atggtttcgg cgcacatagc
    21061 gaccttgaat ggattgaagc ggcggcggat gttgtgaagc acgccaaaat cgcgacgttg
    21121 ttgctgccag gaatcggcac tattcacgat ctgaaaaatg cctggcaggc tggcgcgcgg
    21181 gtggttcgtg tggcaacgca ctgtaccgaa gctgatgttt ccgcccagca tattcagtat
    21241 gcccgcgagc tcggaatgga caccgttggt tttctgatga tgagccatat gaccacgccg
    21301 gagaatctcg ccaagcaggc aaagctgatg gaaggctacg gtgcgacctg tatttatgtg
    21361 gtggattctg gcggtgcgat gaacatgagc gatatccgtg accgtttccg cgccctgaaa
    21421 gcagtgctga aaccagaaac gcaaaccggc atgcacgctc accataacct gagtcttggc
    21481 gtggcgaact ctatcgcggc ggtggaagag ggctgcgacc gaatcgacgc cagcctcgcg
    21541 ggaatgggcg cgggcgcagg taacgcgccg ctggaagtat ttattgccgc cgcggataaa
    21601 ctgggttggc agcatggggc cgatctctat gcgttaatgg atgccgctga cgacctggtg
    21661 cgtccgttgc aggatcgacc ggtacgagtc gatcgcgaaa cgctggcgct gggatacgct
    21721 ggtgtttact ccagcttcct gcgtcactgt gaaacggcgg cggcgcgtta tggcttaagc
    21781 gcggtggata ttctcgttga gctgggcaaa cgccggatgg ttggcggcca ggaggatatg
    21841 atcgtcgacg tggcgctgga tctgcgcaac aacaaataat attgactgca cgagaacgtg
    21901 cattttgtat gcgcatccca gcacgactgc cggatgcggc gtgaacgcct tatccggcct
    21961 acatttcgcc cgtaggcagt cattaaatag ttctgattac gcggctggca atgtatcagt
    22021 cgcgattcac ctgcactcgc aacgaggttc atcttatgtc gactcgtacc ccttcatcat
    22081 cttcatcccg cctgatgctg actatcgggc tttgtttttt ggtcgctctg atggaagggc
    22141 tggatcttca ggcggctggc attgcggcgg gtggcatcgc ccaggttttc gcactcgata
    22201 aaatgcaaat gggctggata ttcagcgccg gaatactcgg tttgctaccc ggcgcgttgg
    22261 ttggcggaat gctggcggac cgttatggtc gcaagcgcat tttgattggc tcagttgcgc
    22321 tgtttggttt gttctcgctg gcaacggcga ttgcctggga tttcccctca ctggtctttg
    22381 cgcggctgat gaccggtgtc gggctggggg cggcgttgcc gaatcttatc gccctgacgt
    22441 ctgaagccgc gggtccacgt tttcgtggga cggcagtgag cctgatgtat tgcggtgttc
    22501 ccattggcgc ggcgctggcg gcgacactgg gtttcgcggg ggcaaactta gcatggcaaa
    22561 cggtgttttg ggtaggtggt gtggtgccgt tgattctggt gccgctatta atgcgctggt
    22621 tgccggagtc ggcggttttc gctggcgaaa aacaggctgc gccaccactg cgtgcgttat
    22681 ttgcgccaga aacggcaacc gcgacgctgc tgctgtggct gtgttatttc ttcactctgc
    22741 tggtggttta catgttgatc aactggctac cgctgctttt ggtggagcaa ggattccagc
    22801 catcgcaggc ggcaggggtg atgtttgcac tgcaaatggg tgcggcgagc gggacgttaa
    22861 tgttgggcgc attgatggat aagctgcgtc cagtaaccat gtcgctactg atttatagcg
    22921 gcatgttaac ttcgctgctg gcgcttggaa cggtgtcgtc atttaacggt atgttgctgg
    22981 ccggatttgt cgcggggttg tttgcgacag gtgggcaaag cgttttgtat gccctggcac
    23041 cgttgtttta cagttcgcag atccgcgcaa caggtgtggg aacagccgtg gcggtagggc
    23101 gtctgggggc tatgagcggt ccgttactgg ccgggaaaat gctggcatta ggcactggca
    23161 cggtcggcgt aatggccgct tctgcaccgg gtattcttgt tgctgggttg gcggtgttta
    23221 ttttgatgag tcggagatca agaatgcagc cgtgtgccga tgcctgacgc gccttattaa
    23281 gcaggggcga aagggagttg cccccaatac acccacggcg tttacaggta tactcgctaa
    23341 aaattattca gcgggtttgg aaacaaagat ggcaaaactt accttacaag agcagttgct
    23401 caaagcagga ttagtcacca gcaaaaaagc ggcgaaggtc gagagaacgg cgaaaaaatc
    23461 gcgcgttcag gcgcgtgaag ctcgggctgc ggtagaagaa aataaaaagg cacagcttga
    23521 gcgtgataaa cagcttagcg aacagcaaaa acaagcggcg ttggcgaaag aatataaagc
    23581 ccaggtgaag cagctcattg aaatgaaccg aatcaccatt gccaatggcg atattggttt
    23641 taacttcact gacggcaatc tgattaagaa gatttttgtc gataagctca cacaggcgca
    23701 gttgattaat ggtcgtctgg cgattgcccg cttgttggtc gataacaata gcgaaggtga
    23761 atacgccatt attcccgcca gcgttgccga taaaattgct cagcgtgatg ccagcagtat
    23821 tgtgttgcac agcgcgctca gtgccgaaga gcaggatgaa gacgatccgt atgccgactt
    23881 caaagtgcct gatgatttga tgtggtaaac atagataagt cgtgcgcaga tgcctgatgc
    23941 gacgctatgc gcgtcttatc atgcctacgg cctgcgggcg aaatgtaggc ggataaggcg
    24001 ttcacgccgc atccggcagt cgtgcactat tatcaacgca tattcagttt attggcgtgg
    24061 taggcaatat gctcgccaat aaaactggag acaaaataat agctgtggtc gtaaccctcg
    24121 tgataacgga ttaacgtctt gatattcatc tcctggcaga tcttttcaag atttggagtc
    24181 cgcagctgtt ctgcgtaaaa atcatcactc aacccctgat caaccatgat ttccgcaacg
    24241 cgttgacctt gtgaaataag actcaccgga tcgtaatcca accaggcctc tttattttca
    24301 ccaagatatg cggcaaaggc ttgctgtccc cacggtactt gcgatgggga gacaatgggc
    24361 gaaaacgccg agacgctgac atattcatct gggttacgta acgccagcac cagcgcgccc
    24421 agcccgccca tagaatgacc tgagatagac tttctggtcg ttgccgaaaa atgttgcatc
    24481 actaaatccg gcagctcgtt gcggatatag tcatacattt tgtaatgttc attccacggc
    24541 gcttgcgtcg cgttcaggta aaacccggca ccttgcccga gatcgtaacg gtctgcatct
    24601 gcgacatgac tgcctcgcgg actggtgtcc ggcgcaacaa caataatgtt gtgctcagcc
    24661 gcgtaacgct gcacccccga tttagtaatg aaattctgct cattgcaggt caggcccgaa
    24721 agccagtaca gcaccggtaa tttttcacct gcagcttttg gtgggagata gacgccgaca
    24781 ttcatttcac atttcagtga tgcggaataa tgccgataca cattttgcca gccgccaaag
    24841 ctggcatgtt tttcaatgag ttccatcagt cattatctca cggttaatcc cccggacggc
    24901 tgcccgggga gagtattaag aaccccgtct gaagtggacg gggtaaacca gcgggaaatc
    24961 agtaacgaat tacggttcga atggatttgc cttcatgcat caggtcgaag gcgtcattaa
    25021 tttcatccag gctcatggta tgcgtgacaa acggttccag atcgatatca cctttcatcg
    25081 catcttcaac catacccggt aactgggaac gacctttcac gccgccaaac gcggaacctt
    25141 tccatacgcg accagtgacc aactggaatg gacgggtgga gatttcctga ccggaacccg
    25201 cgaccccgat gatcaccgac tgaccccagc cgcggtgcgc actttccagc gccgcacgca
    25261 tcacgttgac attaccgatg cattcaaagg tatggtcgat accccatttg ttgatatcca
    25321 gcaggacatc tttaatcggt ttgtcgtagt cattcgggtt aatgcagtcg gtggcaccga
    25381 agcgacgcgc cagatcgaat ttcttcgggt tggtatcgat agcgataatc cgtcccgctt
    25441 tcgcctgacg cgcgccctga accactgcca gaccaatcgc gccaagacca aacacagcaa
    25501 cagaatcccc tggctggact ttagctgtgt tgtgtaccgc gccaataccg gtggtcacgc
    25561 cacagcccag caggcagacg tgttcatggt ttgcttctgg attaattttt gccagagaca
    25621 cttccgcgac tacggtgtat tcactgaatg tagagcaccc catgtagtga taaagcggct
    25681 gcccgttgta agaaaaacgg gtggtgccgt ctggcatcag gcctttaccc tgggtttcgc
    25741 gaaccgcaac acagaggtta gttttgccag aacgacagaa ctcacactcg ccgcactctg
    25801 cggtgtaaag cgggatcaca tggtcgcctg gtttgacgct ggttacgcct tcaccgactt
    25861 caaccacaac gccagcccct tcgtgaccga gaaccaccgg gaatacacct tccgggtcat
    25921 cgccggagag ggtaaatgcg tcggtatggc aaacgccggt atgggtgact ttaatcagca
    25981 cttcaccttt tttcggtggt gcaacgtcaa tttcaacgat ttccagcggt ttaccgggag
    26041 caaatgcaac ggcagcacgt gatttcatct ctcgctcttc ctcaatatgg taatagattc
    26101 agctatttaa gataggcacg aaccagttca atagtgtcgt caacggattg gctgacttcg
    26161 cggctgtagc agtcatttcg gtcaaacgtt tcccggatat ggctttcaag cacttctgcc
    26221 atcagcccat tagccgcgcc ccgaacggca gcgatctgtt ggagtatggc acggcattcg
    26281 gcatcaccct ccagcgaccg ttccagagca tcaatctgcc cccgaatacg acgaactcga
    26341 gtaaggacct ttttcttctc ttccggagta ctgggcattt cgcacctcat catctgcacg
    26401 caatatacta taggggggta tgctatatgt caatacatac ccccctatag tatataacac
    26461 tggagaataa ttgttgtccg aagcctaacc aggtgaacgg tccctcaaga cagatgggga
    26521 ccgtttatgt gctattttta tgccgtatcc gctaaaacaa acatcccgta aggatggatt
    26581 tcgaggtaat agtgctcacc aatatcgggc tgtaaacggg tggcgtttac ttgtaataaa
    26641 atctcctgtc catgccactc tacggtcact tcgtactgcg gccccatgta ggcgacatgg
    26701 cgaataacac aacgctgact ctcttcgccg tgattgctta aggtgatggc ttccgggcga
    26761 acgccgacgg tgccgctacc ttgcgccggg aaatgcgccg cgcgcggcag gcgataaccg
    26821 tagatatcga catactcttc gctaaagttc gccgggaaca ggttggcgtc gcccataaaa
    26881 cttgccataa atcgggaggc tggctgacga tagagatcct gcggtgagcc aatctgcatg
    26941 atgtgacctt tattcatcac cagcacagtg tcggacaccg cgaaagcttc actttgatcg
    27001 tgagtgacgt agagcgacgt gatattaaac tgcttttgca gctcgcggat cttatcgcgc
    27061 atactgcggc gcaggttggc atcgaggtta cttaacggct catcaaacag cagcaccttt
    27121 ggcttgagga tcaacgcgcg ggccagtgcc acacgctgtt gctgaccacc ggaaatctga
    27181 tcgacatagc ggtcctcgaa gccttccaga tccaccattg ccagcgcctc cttcacccgc
    27241 tgtttcactt cgctacgcga gacgccaagc atcttcagcc cgtagccaac gttttcaccc
    27301 agcgacatat gcgggaacag agcgtatgac tgaaacacca tgcagatatc gcgctgttga
    27361 atggaacgat gcgtgacatc ttcgccatca ataaaaattt gcccttcgct cggtttttcc
    27421 agcccggcaa ccagacgcaa aatggtggtt ttgccacagc cggaaggacc gagcagcgtc
    27481 accatttgcc cttgtggaat ggtgagattg atattgtcga taaccgtatt actgccgaat
    27541 cgtttagtga cgttgcgcag ttcaacaaaa tttttctgac tcatattgcg ctccattacg
    27601 cctggttttt ggctttagaa cgggagatac gagcttctcc gatcagccag tcaaagagga
    27661 aaataatcgc cagcatcacc acgatcagaa tcgaaccata ggcgatggct acgccatatt
    27721 cgccgtcttc aacgcggtta agaatgtagg cggttgccac gcgggtgtcc ggcgtgacga
    27781 gaaagacgat ggcgctgacc gtcgtgatgg cccgcacaaa gctgtagatc agggccgaga
    27841 gaatggccgg acgcagcagc ggcagtaaaa tgtgggttat ggttcgcaag gaacccgcgc
    27901 gcaggctgag tgaggcttca tccagcgatt tgtcgatctg ccccagaccc gcaatcccgg
    27961 cgcgaatacc caccggaacg ttacgcatca ccatcgagat aatcacaatt gccgccgttc
    28021 cggtgagata aaccggagcg ctgttaaagg caagaatata cgaaacgccc gccaccgtac
    28081 ccggtacggc aaagcacagc atggtggtga actcgatggt ctttttgcct ttgaattgtt
    28141 gacgcaccac cacccaggcg atgagcaggc caaagatagc ggtaatggga gcggcaatcc
    28201 cggcgtagag cagggtatcc agcagtgaag gccacgcgcc gtcgctcatt ccctgaccaa
    28261 atagcttgat aaagttatcc agcgtcaggg tgtagtccac gccccagttc accgtgaagc
    28321 tgccgtagaa aatgctgccg tagagcaggg cgttaaaggc aatccatatc gcaagtatgg
    28381 cgaccacgct ccacaccagg gtgaccggta gcggctggac gtcgccacgg taggatttgc
    28441 cggaaaccgt gacgtaagag cgtttaccaa tccacatata ctggatgcag aacaccagca
    28501 gcgagaacag cagcaggaaa gcgccaaggg tgctggcggc ctgataatcc agctgcgagc
    28561 cggtaatata gaagtaaatt tgcgtcgcca gtacgtcgaa gttaccgcca agtaccagcg
    28621 ggttactgaa atcagccagt gactgtacca caacgattaa aaaggcattg gcgagtgcgg
    28681 gcttcaacaa cggcacaaac acgccgttaa aggtttgcca gcggctggcg cgcagggtgt
    28741 aggaggcctc ttccagcgag ggatgaatgg ttttaatggc cccgtcgagg atcataaatg
    28801 ccattggcgt gaatgccaga acctgtgcca gccagatccc ggtaaaaccg tacagccagt
    28861 tggtgttggt cagcccaaac cactccacca taaattccgt aacatagccg gagcgcccca
    28921 tcatgagcgt cacgcctaag ccgaccacaa acggtggcgt aacgatgggc aaaatggaaa
    28981 agatgcgacc aataatggcg ctgcgtttag cgatgcgcgt ggtataaatc gccagcacca
    29041 ggccgaagaa agtacagcct atccccaccg ctatcgacaa gacgatagag ttgacgatca
    29101 cctgaacaat atgtgcctgc gagagcacgg tcataaacgc cagcggggcg aactctcccg
    29161 cgtcgttggt gaacatcggg ataaaaatgg cgatgctcgg ccagacgata aaaatgccaa
    29221 tcagcgccac aatcgcaatc agtgagccga tcacgaagcg gtcgccgccc agccattcga
    29281 ggcgggttaa ggccagcgtc ataatcgcgc ctagttcaac aaacagcacg atggcggcgt
    29341 agcctaaacc gcgtccagcc acggtggcgc tgatgacgat aaacgccata cagagcaatg
    29401 cccagctggc atcaaaataa tgacgactac gctgttcgcg cgtggcctca cgaaacgggc
    29461 gaaccagcaa cagactcggt aacaaatacc acagccagct gatattaaaa tgcgaccagc
    29521 cataagcctc gaggatctca tcactcgtgg actccagcag gccataatcg aggctccagc
    29581 tcggcaacag tataaatgcc agccagccca gcaaaaccca aaggaaaatc gcatcccgct
    29641 ttttcacggg atggagtgca agtgtctgtg acatagcagt tccggtatgg ttggccgggg
    29701 caggcgtttg tgctgcaccc ggtaaatacg cctaatgcgg gtttatttac ccatcttcac
    29761 ttcgctgacc catttattaa tcaatgcctt acgaacatcc gtggaaccat atttatccat
    29821 gtcgtagttg attaatttca ggtcgtcgag cttcagcgaa tttggcgacg tttcggcggt
    29881 ggtattggtc aggatctgat aagacttccc tttcttccac gccagttcct gagcttcttt
    29941 tgataatacc cagtccacaa acagtttggc gttgtcgagg ttgcgcgcgc ctttcagaat
    30001 gctgacgccg ccaatttcgt agcccgtacc ttcacacggg gaaatcaact ccagcggcgc
    30061 gccctgttct ttttccagcg agtagtcatg caggaagcca ataccaatcg ccgtttcgcc
    30121 acgggcggcg ttacgtgccg gggctatgcc tgatttggtg tactgagaga cgttggcgtt
    30181 cagttgtttt agataatcaa aggcctgatc ttccccccac agctgcgcaa aagtagccag
    30241 ggcggtgtag gcagtaccgg aactttgcgg atcggcaatt tgaatttcgc ctttatattc
    30301 cggtttggtg agatctttcc agcacttcgg caccggcagg tttttctctt tcagacgatc
    30361 ggtgtttacg ccaaaaccga ggatccccac ataaacggca gaggacagat tccctttcac
    30421 ttttgccgga tcgcggaatt tctccatgat ctgctcaagg ttaggggatt tatacgcctg
    30481 tagcaggccc atctcgccag cctgcgattg cgggtctaaa gtgccgccgt accagacgtc
    30541 agcctggggg tttttctttt cagcatcgac ttttgccagg gtgcttccgg aaccgttgcg
    30601 gataaacgag gtttttacat catatttctc gccaaacgct ttggtttcgg cctcacacat
    30661 ttcattggtg gcgctgcaat agaccaccag ccggcctttt gcctgcgcgg cacccgttaa
    30721 agtcgccagc gcaacggcag aagcaatcag agtagaggta aaggtcattt tcattgtttt
    30781 atccttcgag cgtgagtggt ggtgatgccc gccatcagta acggcatcag cagtaaaccc
    30841 ataagtactg cggcgaccga gagtaaactg aacattcccg gccagccgta gcgttcgatg
    30901 accagcgaca gcggccagcc tgccagcgcc gcgccaaggt aagcgaacag gccgagaaat
    30961 ccggtgattg aacccgccgc agctttgtgc ccacactcca ccgccgcaag tccaatcagc
    31021 atttgtgggc caaagacgaa aaagcccacg gtaaaaaagc acactgccag cagagcgtag
    31081 tgatgaaccg gtgccagcca cagtgcggcg actgacacca tcaaccccaa cgtgaacagc
    31141 aaaatcattg gcgctcgttg cccgctaaac agcaaatccg atccccatcc ggcaaataac
    31201 gcgccgagta agccgcccac ttcaaacagc attaccgtgg cgttggcgct gagcaggttg
    31261 acaccgtggc tttccgtcag ccagatattg ccccagtcgt taagcgcaat ccggatcaca
    31321 taaaccagta cataagagac gcccagtagc cagatcagcg gattttgcag catggtggtg
    31381 cgcaacattt gccacagccc catcggcgga ctttgttgtt cctgacgtaa ctccagcgga
    31441 tcgtgacgcc agtgaccaac tgtcggtaat ccctcttcct ggggcgtgcc tttgagttgt
    31501 agcgttaacc agatccctaa tgccatgctg attatccccg gtgtcagcat cgcggcctgc
    31561 cagccccacc agtgggcggc aaaggcgcta atcagcggaa taatcgctcc gccaatgttg
    31621 atcgacatat tccagcagcc ccaccagaaa ccgcgctcat tgcgtgagta ccagtgggtc
    31681 agcaagcggg cacacggcgg ccatccccag ccctgaaaaa aaccgttcag tgtccagacc
    31741 acaagcagaa gcgtaagtga ttcgccaaag gcaaacacca cgttcaataa cccggtggcg
    31801 aacaagccga cgcccataaa accgcgttgt ccgtggctgt catgccacag tccggcggta
    31861 aatttcgaca atccgtagct gagataaaac agcgaaccca gcaggccaat gtcgccttta
    31921 tccagtccga ggtcggtttg tagcgccgga aggacgtaat tcacgctttt tcgcgtcaga
    31981 taaaatgcgg cgtagccaat aatcatgcac atcagcaatc gcggacgtaa tgtccggtag
    32041 cattgattaa tttctctcgc tgaacgcgcg tgcatggttg tctccatgtt gctgactata
    32101 tgaaaatgaa agtgaaaggg gatgagagaa aatccgggat acctaagact ttttcctggt
    32161 tatccggcga tttgttgcaa aaatgtgggc aagttaacaa ttacccgcgt gccgtgctgt
    32221 ttttcgagcg ttaagtcgcc gcccagggcg ctgacgcgct cacgcatccc ctgaatgcca
    32281 aatccggcca ttttcccgct ggcgatcccc acaccgttgt cgctgacttc cagatgcaat
    32341 acctctccct gctggcgcag gataatcgtc acttcgctgg cttcggcgtg tttacagatg
    32401 ttattgagca actcctgcaa taaccgatac aaggtaaagc gcaccgtttc gttttctggt
    32461 gtggagttca gttgataggc aaactggcaa tgaatgccgc gttcactaaa ggcaaattca
    32521 ttaatcagat gcagcaacgc ttcgcgaaat gtcagttcat caagcgcggg aggccgcaac
    32581 tggcgcagca attggcgggt ggagagatga atgcggcggg cgagctcatt gatttggctg
    32641 gctgcggatt gaatttgcgc cggatcacgc gcccgcttca ccagttgcga ctggatctga
    32701 atggcggtga tgttctgacc gatttcgtcg tgcagctctc gcgccaggtt ttttcgcgtg
    32761 tcctcctcgg tgtggatcag cttttccgcc aatgcccgcc gcgctgccag ctcggcctcc
    32821 agccgttgtc ggtaatggtg caggtttagc gccagatgtt gctggcgact gatggcaatg
    32881 cccaggccaa ttcccagcaa ggcctgggtc gcgaggaaaa tctccagctc caccaggtta
    32941 ctaaagccca cgccaatctg gcgggcgatg gtgatcatca tgctgcccaa aagcccggag
    33001 agtacgccac cttgccagcc aaatttccac gccatcacca cattgggcaa aaagaccacg
    33061 atcaacagca gacgttcaat ttcaggtgac agcaccatct gagtgccgat accaataata
    33121 aaaaacaggc tacaccagat gattaaagag gtacgcaagg ggggattatg cgtatcgagt
    33181 cccaggagct ggtagcgatg ctgctggcgc agaaactcaa agataagata gacaaacggc
    33241 gtcagtaata cgccgccggt aaatgaggtc aaacccggca gcattattgg gcctgacata
    33301 aaaggggaga gcagggcggt attcaacaat gaagctgccg tgacagtggc taacagcaac
    33361 gtcagacgct gccagtaaag tggaaaacgg tgccagaagt tttgcgcaag gaggacagga
    33421 aacaggctaa taaccggcgt tgccagcatg agataaccgt tcaatagctg ttcactatgg
    33481 agccagaaca tcatcagcaa aggggggagc accaacgcag gccagtagcg gcgagagagc
    33541 aaaataacaa gggcgagata aactccctgc gggagaaaca acaccgcctg ttgaccgtta
    33601 ttggtcagat aaaagctgag cgtccacagc atcagccagc cggttcccca ggccagcaca
    33661 ataaacagcg aaatcaccca atgacgaaga ttgcgcgaca ttaatgcccc gccagcagat
    33721 ggtggtcgag cgcaaaatgt accagttcaa tggtgctatg gcagttcagt tttcccagca
    33781 cattggcgcg atgcacatgt accgttttat gactgagttc gagtttaaag gcgatctctt
    33841 taacgctgtc tcctttgacc agtaaatcaa acacttcacg ttctcgtggg gtgagaattt
    33901 ccagcgcctg ggcgggttgc ccgccgccgc gtaaagcccg aatcgcatcg gcacagagat
    33961 aatgcccgcc cagcccgacc gagcgcaccg cctgtactaa ctcttccggc ccacagcgtt
    34021 tggttaaata cccgctggca ccggcatcca gcgcgctttg cacaaaggtt ggcgcgtcat
    34081 aaatgctaag aataatggcg cgaaattgcg gtttttgtgc acgcaggcgc ttaagcaaac
    34141 tgaggccgtt ttcgtccggc atggcaatat caatgacggc aacattgacg tcatcacgga
    34201 ttaatgccga ccatgcctgc gcggactact gtactgacca atgacctcca gatcgtcttc
    34261 cagagagagt aattgtgcga agccagagcg caccaccaca tggtcatcca ccagcactac
    34321 ccgaatcata cttttcttct cttattatct aattctccta tcatgcatca cggcgcggcg
    34381 ctggcaatca accagcgaca gatatggaat ggatgtaaca aaatggcaat aatttgccgg
    34441 agcgcgcaca tcttatctgg cttgtgaacc ggcgggatac gttcgccaaa accgcaaaat
    34501 gcgcaaactc gctaaccatt gccgattttt ttctaaaaac gctttgttct taacaagacc
    34561 ttttaattcc tttatcaaac tgacccgcaa cgaaatactg ctcccataac aagacagggg
    34621 agcagacaat catggcaatt tcatcgcgta acactcttct tgccgcactg gcattcatcg
    34681 cttttcaggc gcaggcggtg aacgtcaccg tggcgtatca aacctcagcc gaaccggcga
    34741 aagtggctca ggccgacaac acctttgcta aagaaagcgg agcaaccgtg gactggcgta
    34801 agtttgacag cggagccagc atcgtgcggg cgctggcttc tggcgacgtg caaatcggca
    34861 accttggttc cagcccgtta gcggttgcag ccagccaaca ggtgccgatt gaagtcttct
    34921 tgctggcgtc aaaactgggt aactccgaag cgctggtggt aaagaaaact atcagcaaac
    34981 cggaagatct gattggcaaa cgcatcgccg taccgtttat ctccaccacc cactacagcc
    35041 tgctggcggc gctgaaacac tggggcatta aacccgggca agtggagatt gtgaacctgc
    35101 agccgcccgc gattatcgct gcctggcagc ggggagatat tgatggtgct tatgtctggg
    35161 caccggcggt taacgccctg gaaaaagacg gcaaggtgtt gaccgattct gaacaggtcg
    35221 ggcagtgggg cgcgccaacg ctggacgttt gggtggtgcg caaagatttt gccgagaaac
    35281 atcctgaggt ggtgaaagcg ttcgccaaaa gcgccattga tgcccagcaa ccgtacattg
    35341 ctaacccaga cgcgtggctg aaacagccgg aaaacatcag caaactggcg cgtttgagcg
    35401 gcgtgccgga aggtgacgtt ccgggactgg tgaaggggaa cacctatctg acaccgcagc
    35461 agcaaacgac agaactgacc ggaccggtga acaaagcgat catcgacacc gcgcagtttt
    35521 tgaaagagca gggcaaagtc ccggctgtag cgaatgatta cagccagtac gttacctcgc
    35581 gcttcgtgca ataaaaggag gcgcagatgc tgcaaatctc tcatctttac gccgattatg
    35641 gcggcaaacc ggcgctggaa gatatcaacc tgacgctgga aagcggcgag ctactggtgg
    35701 tgctggggcc gtccggctgc ggtaaaacca ccctgctgaa tctgattgcc ggttttgtgc
    35761 cttatcagca cggtagcatt caactggcgg gtaagggtat tcagggaccg ggagcagagc
    35821 gtggcgtagt ttttcagaat gaagggctac taccgtggcg caatgtacag gacaacgtgg
    35881 cgttcggcct gcaattggca ggtatagaga aaatgcagcg actggaaatc gcgcaccaga
    35941 tgctgaaaaa agtggggctg gaaggcgcag aaaaacgcta catctggcag ctttccggtg
    36001 gtcaacgtca gcgggtgggc attgctcgcg cgctgacggc aaatccccag ctgttattac
    36061 tcgacgaacc gtttggtgcg ctggatgcct tcacccgcga ccagatgcaa accctgctgc
    36121 tgaaactctg gcaggagacg ggcaagcagg tgctgttgat tacccacgat atagaagaag
    36181 cggtgttcat ggcgactgaa ctggttctgc tttcacccgg ccctggccgc gtgctggagc
    36241 ggctgccgct caactttgct cgccgctttg ttgcgggaga gtcgagccgc agcatcaagt
    36301 ccgatccaca attcatcgcc atgcgcgaat atgttttaag ccgcgtattt gagcaacggg
    36361 aggcgttctc atgagtgtgc tcattaatga aaaactgcat tcgcggcggc tgaaatggcg
    36421 ctggccgctc tcgcgtcagg tgaccttaag cattggcacg ttagcggttt tactcactgt
    36481 atggtggacg gtggcggcgc tgcaactgat tagcccgctg tttttgccgc cgccgcaaca
    36541 agtactggca aaactgctca ccattgccgg accgcaggga tttatggacg ccacgctgtg
    36601 gcagcatctg gcagccagcc tgacgcgcat tgtgctggcg ctactcgcgg cggtgttgat
    36661 cggcattccg gtcgggatcg ccatggggct aagcccgacg gtgcgcggca ttctggatcc
    36721 ggtaatcgaa ctttatcgtc cggtgccgcc gctggcttat ttaccactga tggtgatctg
    36781 gtttggtatt ggcgaaacct cgaagatctt actgatctat ttagcgattt ttgcaccggt
    36841 ggcgatgtcg gcgctggcgg gggtgaaaag cgcgcagcag gttcgcattc gtgccgccca
    36901 gtcgctgggt gccagccgtg cgcaggtgct gtggtttgtc attttgcccg gcgcgctgcc
    36961 ggaaatcctc accggattac gtattggtct gggtgtgggc tggtctacgc tggtggcggc
    37021 ggagctgatt gccgcgacgc gcggtttagg atttatggtt cagtcagcgg gtgaatttct
    37081 cgcgactgac gtggtgctgg cggggatcgc ggtgattgcg attatcgcct ttcttttaga
    37141 actgggtttg cgcgcgttac agcgccgact gacgccctgg catggagaag tacaatgagt
    37201 gaacgtctga gcattacccc gctggggccg tatattggcg cacaaatttc gggtgccgac
    37261 ctgacgcgcc cgttaagcga taatcagttt gaacaacttt accatgctgt actgcgccat
    37321 caggtggtgt ttctgcgcga tcaagccatt acgccgcagc agcaacgcgc gctggcccag
    37381 cgttttggcg aactgcatat tcaccctgtt taccctcatg ccgaaggggt tgacgagatc
    37441 atcgtgctgg atacccataa cgataatccg ccagataacg acaactggca taccgatgtg
    37501 acatttattg aaacgccacc cgcaggggcg attctggcag ctaaagagtt accttcgacc
    37561 ggcggtgata cgctctggac cagcggtatt gcggcctatg aggcgctctc tgttcccttc
    37621 cgccagctgc tgagcgggct gcgtgcggag catgattttc gtaaatcgtt cccggaatac
    37681 aaataccgca aaaccgagga ggaacatcaa cgctggcgcg aggcggtcgc gaaaaatccg
    37741 ccgttgctac atccggtggt gcgaacgcat ccggtgagcg gtaaacaggc gctgtttgtg
    37801 aatgaaggct ttactacgcg aattgttgat gtgagcgaga aagagagcga agccttgtta
    37861 agttttttgt ttgcccatat caccaaaccg gagtttcagg tgcgctggcg ctggcaacca
    37921 aatgatattg cgatttggga taaccgcgtg acccagcact atgccaatgc cgattacctg
    37981 ccacagcgac ggattatgca tcgggcgacg attcttgggg ataaaccgtt ttatcgggcg
    38041 gggtgatacg agagtggacg gtcccctcgc ccccttgggg agagggttag ggtgaggggg
    38101 cgttcaccgt actttcaaca ggttaactcc ccctttctga gaggaaacaa aattaacgca
    38161 gaatcttctt ctcagccaaa tccagcgcaa agtagctgaa aatcagatcc gcacctgcac
    38221 gcttaatcga acctaagctt tcgagcacga ctttctcttc atctatagca cccgccagcg
    38281 cggcgaactt aatcatcgca tactcaccgc tcacctgata cgcgccaatc ggcaattcag
    38341 tacgttcacg cagctcacgc acgatgtcga ggtacgctcc agcaggttta accatcaggc
    38401 agtctgcgcc ctgggcttca tccagcagtg actcacgaat cgcctcacga cggttcattg
    38461 ggttcatctg atagcttttg cggtcgcctt ttaatgcgct tccggcagct tcacggaacg
    38521 gaccataaaa ggaggaggcg aacttggtcg aatacgacat aatcgccgta tctttaaagc
    38581 cagcagcgtc cagcgcctgg cgaatcgcct gtacctggcc atccatcgcg gcggaagggg
    38641 cgatgaagtc tgcacctgca gcagctgcaa ccacggcttg cttgcctaaa ttttccagag
    38701 tcgcgtcgtt gtcgacgcca tgctcgcaca gcacaccgca gtgaccgtga gaagtgtatt
    38761 cacagaaaca ggtgtctgac ataacaatca tttctggcac tgtctgcttg cagatgcgcg
    38821 acatacgcgc caccagtcca tcttcccgcc aggcatcgct gccggtttca tcggtatggt
    38881 gagagatgcc aaaagtcatc acggaacgaa taccggcgtt ggcgatgcgt tcaatttcgc
    38941 gtgccagatg tttctctgga atgcgcatca cgcctggcat ggcttcaacg gctttgtagt
    39001 cgtcaatttc ttcttcaaca aagatcggca acaccaggtc gttaaggcta agtgttgtct
    39061 cttcaaacat agcgcgcagc gcaggagatt tgcgcaggcg acgagggcgt tggattaagt
    39121 ctgtcatggt ctgcctgatg tttgtggaat taaggtgcac agtatacctg aagcggggca
    39181 ggagtgtttt acgaaagttg tcacgatgtt gacaagaaga gaatgaaaga gagaccaggg
    39241 cat
//
LOCUS       DAPGRN010000034        38915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPGRN010000034 DAPGRN010000000
VERSION     DAPGRN010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.034"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..236)
                     /locus_tag="RHW33_001899"
     CDS             complement(<1..236)
                     /locus_tag="RHW33_001899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645402.1"
                     /translation="MSDNTRLNVWERNSIPVMEFCSLKRAAELLNCQIDDLIHFAQIG
                     IIEFCLELNGFEAALMMMRKGGDFKDWENMYPPRV"
     gene            complement(311..1582)
                     /locus_tag="RHW33_001900"
     CDS             complement(311..1582)
                     /locus_tag="RHW33_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV1645403.1"
                     /translation="MALNELSPKKVEKAKPKETDYKLTDGGSLYLLVKKNGTKAWRMN
                     YRFNGKQATLALGVFPDVSLALARKRRDEARQLLADGVDPRVSKKAAPTEPASATFQT
                     IALEWHKGSIGHPSWKEITRTKILREMENHIFPYIGNKPIDSLKTSDLLPLLVRMSDQ
                     GIGATTGRVKTTLASVFRYAIQRGIVEYNPTHDLRGAISSPKIKHRPALPLERLPELI
                     SKIDSYTGRPLTKLAVLFSLHTFVRSSELRHARWEEINFENAMWTIPAQRKRIDGVKY
                     SERGAKMGSIHYVPLSNEAIDVLEAIKKISGEYELIFPGDSNPYKPMSENTVNKSLRI
                     MGYDTQVDICLHGFRAMACSALTESGLWSRDAVERQMSHQERNEVRAAYVHLAQHMHE
                     RRRMMQWWSNYIEANTDRYIAPYKMKKLRVV"
     gene            complement(1855..2055)
                     /gene="torI"
                     /locus_tag="RHW33_001901"
     CDS             complement(1855..2055)
                     /gene="torI"
                     /locus_tag="RHW33_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HDV1645404.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2113..2280)
                     /locus_tag="RHW33_001902"
     CDS             complement(2113..2280)
                     /locus_tag="RHW33_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645405.1"
                     /translation="MYFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHQTA"
     gene            complement(2353..2637)
                     /locus_tag="RHW33_001903"
     CDS             complement(2353..2637)
                     /locus_tag="RHW33_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV1645406.1"
                     /translation="MANLQLSVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDIPYDGYEIKTITHPHFGDKPVNVFAIKVNIGNE"
     gene            complement(2630..3268)
                     /locus_tag="RHW33_001904"
     CDS             complement(2630..3268)
                     /locus_tag="RHW33_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
                     /protein_id="HDV1645407.1"
                     /translation="MSAITKERIKLFIKNPLDNGLTRGEQMELARIALASLEREQIRH
                     EHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWADMQFLLWDAQRRAGISDA
                     EITAAMEDKLKINMERQWPEPKDGEPRLHIKEPGNYPVTPDGWISCSERMPDDGQHVI
                     ILCDGAFVLYAQYRDGEFFDVVRDGDEFFETQSRNVTHWMPLPEPPQEVNRG"
     gene            complement(3270..3791)
                     /locus_tag="RHW33_001905"
     CDS             complement(3270..3791)
                     /locus_tag="RHW33_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV1645408.1"
                     /translation="MSKIDYQALREAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVVLALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEAEKQRAKDLFMENARLKSGIAGLIHLGIRYADVEVMRIAGDAQLSTPCTDSI
                     INSIATGIRIKGE"
     gene            complement(3788..3955)
                     /locus_tag="RHW33_001906"
     CDS             complement(3788..3955)
                     /locus_tag="RHW33_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDV1645409.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRKGGSQ"
     gene            complement(3952..4233)
                     /locus_tag="RHW33_001907"
     CDS             complement(3952..4233)
                     /locus_tag="RHW33_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645410.1"
                     /translation="MKLNIDLGKYVITGTKHDLILSERGIIKEGDNAGKETLSRIGYY
                     SKFEHLVKELCNREILLSQAQTLQDIQQHIETLGVSLSIAIDQFVESKP"
     gene            complement(4253..4546)
                     /locus_tag="RHW33_001908"
     CDS             complement(4253..4546)
                     /locus_tag="RHW33_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDV1645411.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDLIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(4570..4953)
                     /locus_tag="RHW33_001909"
     CDS             complement(4570..4953)
                     /locus_tag="RHW33_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645412.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNDPQQQSSNQQNTPVYDDSIPF"
     gene            complement(4953..5558)
                     /locus_tag="RHW33_001910"
     CDS             complement(4953..5558)
                     /locus_tag="RHW33_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV1645413.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(5815..5967)
                     /gene="kil"
                     /locus_tag="RHW33_001911"
     CDS             complement(5815..5967)
                     /gene="kil"
                     /locus_tag="RHW33_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDV1645414.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(5952..6083)
                     /locus_tag="RHW33_001912"
     CDS             complement(5952..6083)
                     /locus_tag="RHW33_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HDV1645415.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            complement(6108..7076)
                     /locus_tag="RHW33_001913"
     CDS             complement(6108..7076)
                     /locus_tag="RHW33_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HDV1645416.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVRDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAERERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(7253..7504)
                     /locus_tag="RHW33_001914"
     CDS             complement(7253..7504)
                     /locus_tag="RHW33_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645417.1"
                     /translation="MEFHESAIYDFRANANSVKPQPIAVLFKTMGAWAVLCFASDDTD
                     ARMAIGQEMEMDPTNDEFIIYGAPSNYLLDTCNIYNKAA"
     gene            complement(7513..7818)
                     /locus_tag="RHW33_001915"
     CDS             complement(7513..7818)
                     /locus_tag="RHW33_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645418.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKAEEVESKQNRIYYRKPRSEMGVTCSGRQKQRGKSIPAYYD"
     gene            complement(8186..8554)
                     /locus_tag="RHW33_001916"
     CDS             complement(8186..8554)
                     /locus_tag="RHW33_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645419.1"
                     /translation="MVGTAIASFFGMLAISTIYGLAHAFIAKSLSEKISQAWAHRSAR
                     FMILVIIAIQGISAFILYGSSLYLLYQGATFTPYTSDYGTLYDGSEDITVAWIVFGLS
                     MAVSVVADIIKVILVLTFAD"
     gene            complement(8572..9288)
                     /locus_tag="RHW33_001917"
     CDS             complement(8572..9288)
                     /locus_tag="RHW33_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1645420.1"
                     /translation="MENKKSLTTEQLEDAKRLKALYESKKKELGITQYSIADELGITQ
                     GAVGHYLNGRNALNVEVASGFARLLQVSIADFSQSIAAKVAEQAESLKSDANVRYAGE
                     YRAGKRYPVLSSIQAGSWCEACEPYTIKDIDVWLESDAHIQGNAFWLKVEGDSMTAPV
                     GLSIPEGTFVLFDTGREAINGSLVIAKLSDSNEATFKKLIIDGGNKYLKGLNPAWPLV
                     PINGNCKIIGVAIETKLRLV"
     gene            9395..9589
                     /locus_tag="RHW33_001918"
     CDS             9395..9589
                     /locus_tag="RHW33_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDV1645421.1"
                     /translation="MIRMTLADYAKIHGQAKAASDFGVIQCAISKAILAGRNIMVTVK
                     PDGSVIGEEVRPFPSNKKNK"
     gene            9698..9976
                     /locus_tag="RHW33_001919"
     CDS             9698..9976
                     /locus_tag="RHW33_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV1645422.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVVDDEMARLAQQVASILTKEKRPAVGSSLDA"
     gene            10011..10415
                     /locus_tag="RHW33_001920"
     CDS             10011..10415
                     /locus_tag="RHW33_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645423.1"
                     /translation="MPGQTKQVNTGIKAGDKFETVYPFIFVCTDYQSYDGNVHTDERW
                     IGGCHKNYEPADCGYGDQCFYTADAEGKRILEVLAVVEMPGNWQRRIIYSCHLIEPEG
                     KERKGRKAYTVTESRFLKMSTGYFADYEVEEA"
     gene            10415..10546
                     /locus_tag="RHW33_001921"
     CDS             10415..10546
                     /locus_tag="RHW33_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HDV1645424.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQK"
     gene            10554..11414
                     /locus_tag="RHW33_001922"
     CDS             10554..11414
                     /locus_tag="RHW33_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDV1645425.1"
                     /translation="MSNLATVTHLRSSQRPVERRVAEVEDGYTRLANALYEELIGADL
                     TKNQSKVAHAICRKTYGYGKKMDRISDSQLAQITRLPRQKVNKAKNELIAMKVILREG
                     QQIGPNKNIEEWQIEGCHYSGDNVTALVTKSVTKTVTALSPKQGHTKETITKEKRNNK
                     NTMSESVRTECEKSPDRHEETDKAFEEIFWCAGMRKAGKKNAASAFRTQFREWRKTTR
                     GTASEFATMLAEDIACRNGKQFGFDRLLPSSYLNGQRWNDEKPETIQPQSKPSSAITV
                     SKTGYVFFDR"
     gene            11525..12016
                     /locus_tag="RHW33_001923"
     CDS             11525..12016
                     /locus_tag="RHW33_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 motif-containing HNH endonuclease"
                     /protein_id="HDV1645426.1"
                     /translation="MSLEVGEYIPGYEGKYSVTKDGRVYSHLRGRFLNPSPDNKGYLR
                     VTLCSDGIQKTLKVHRLVAQLFIPNPYCKPEVNHINGDKSDNAIWNLEWCTCSENLKH
                     AFSIGLKSAKGVFNGRAKLSETDVIDIRNSVGMKLSELSTKYGVSEMQISTIRRGNAW
                     RHL"
     gene            12013..13893
                     /locus_tag="RHW33_001924"
     CDS             12013..13893
                     /locus_tag="RHW33_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV1645427.1"
                     /translation="MSPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDAKR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVNGHGKTEVVGHMALEAMRQGVKTCIA
                     SLELKPGILLKRLTRQATCCKMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFD
                     YARRRYGIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDS
                     EEKPTGKMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGERMSEKDEQLLASPAS
                     VLMLEKQRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVT
                     EY"
     gene            13970..14176
                     /locus_tag="RHW33_001925"
     CDS             13970..14176
                     /locus_tag="RHW33_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645428.1"
                     /translation="MAEFMLVALKCVGVGWILLTFFIVLHSYIRLVNDGKDPWYTLFG
                     AAFVWVIIGVMPVAVAKMAWRFVS"
     gene            14194..14379
                     /locus_tag="RHW33_001926"
                     /pseudo
     CDS             14194..14379
                     /locus_tag="RHW33_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001527427.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14672..15082
                     /locus_tag="RHW33_001927"
     CDS             14672..15082
                     /locus_tag="RHW33_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV1645429.1"
                     /translation="MKQTIFLRTKQQQQAAINAILETPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIACQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            15079..15261
                     /locus_tag="RHW33_001928"
     CDS             15079..15261
                     /locus_tag="RHW33_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDV1645430.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARKTR"
     gene            15258..15428
                     /locus_tag="RHW33_001929"
     CDS             15258..15428
                     /locus_tag="RHW33_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDV1645431.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCSDCCAELMSD
                     PNSSMYEEEDDE"
     gene            15421..16146
                     /locus_tag="RHW33_001930"
     CDS             15421..16146
                     /locus_tag="RHW33_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDV1645432.1"
                     /translation="MNELINGNAIKMTSIEIAELVESRHSNVKVSIDRLVKRGVIKPP
                     ALQHTNIINDLGVITGKRDFYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGAT
                     AKIPQTFSEALRLAADLEDQKAELEKQLALAAPKVDFADRVGEASGILIGNFAKVVGI
                     GPNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITG
                     RGQQWLTRKLLDNGMLKVTGEAA"
     gene            16146..16436
                     /locus_tag="RHW33_001931"
     CDS             16146..16436
                     /locus_tag="RHW33_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HDV1645433.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSDCHAEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            16433..16795
                     /locus_tag="RHW33_001932"
     CDS             16433..16795
                     /locus_tag="RHW33_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV1645434.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LKLDIFTKSRLRIKVIADAPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            16792..16980
                     /locus_tag="RHW33_001933"
     CDS             16792..16980
                     /locus_tag="RHW33_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HDV1645435.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRP"
     gene            16977..17600
                     /locus_tag="RHW33_001934"
     CDS             16977..17600
                     /locus_tag="RHW33_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV1645436.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDITKTEQLGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            18034..18357
                     /locus_tag="RHW33_001935"
     CDS             18034..18357
                     /locus_tag="RHW33_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDV1645437.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            18341..18817
                     /locus_tag="RHW33_001936"
     CDS             18341..18817
                     /locus_tag="RHW33_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDV1645438.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            18814..19251
                     /locus_tag="RHW33_001937"
     CDS             18814..19251
                     /locus_tag="RHW33_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV1645439.1"
                     /translation="MSRLTAIISALVICVIACLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTEASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLR"
     gene            19239..19391
                     /locus_tag="RHW33_001938"
     CDS             19239..19391
                     /locus_tag="RHW33_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645440.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSESRLNISLLMLDIEHLA
                     RGKGLR"
     gene            19596..20117
                     /locus_tag="RHW33_001939"
     CDS             19596..20117
                     /locus_tag="RHW33_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV1645441.1"
                     /translation="MNIVPLNYKGEPIRFNTDGWINATDIAKRFGKRLDHWLSNAETL
                     EYVRALDEVYSGEPSKILHTRDSGYVKTSKARKDRGGGTWLHPKLSVAFARWCDPKFS
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            20321..20599
                     /locus_tag="RHW33_001940"
     CDS             20321..20599
                     /locus_tag="RHW33_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645442.1"
                     /translation="MLTIILFGAGWKGDVWALESYSPVIKAPDRTFRLLKSSGGAPIF
                     EDAEFDVFEYQLGDEIYLVGCHGNKPSDDVIMSHIQHGDPKPKPYKTL"
     gene            20727..20969
                     /locus_tag="RHW33_001941"
     CDS             20727..20969
                     /locus_tag="RHW33_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HDV1645443.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNATAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            21049..21474
                     /locus_tag="RHW33_001942"
     CDS             21049..21474
                     /locus_tag="RHW33_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HDV1645444.1"
                     /translation="MATEKKNVGRPSDYLPEVADDICALLASGESLVKVCKRPGMPAK
                     ATVFRWLSEHDEFRDKYAKATEARADSIFEEIFEIADNAIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            21471..22886
                     /locus_tag="RHW33_001943"
     CDS             21471..22886
                     /locus_tag="RHW33_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDV1645445.1"
                     /translation="MTSINPIFEPFIEAHRHKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIKDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            22888..25086
                     /locus_tag="RHW33_001944"
     CDS             22888..25086
                     /locus_tag="RHW33_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDV1645446.1"
                     /translation="MAEKKMNDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEEQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLKN
                     KNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTGE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQAAIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILKKSTQQQPQQ"
     gene            25177..26070
                     /locus_tag="RHW33_001945"
     CDS             25177..26070
                     /locus_tag="RHW33_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HDV1645447.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQYQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLDKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            26089..27342
                     /locus_tag="RHW33_001946"
     CDS             26089..27342
                     /locus_tag="RHW33_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HDV1645448.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRPGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKAPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RVPKGTATSITLAAAPAHKVEAKDANDMPVDNRQGTITVSAEGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPPDNADVASRPYANVDANAASSAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            27384..27572
                     /locus_tag="RHW33_001947"
     CDS             27384..27572
                     /locus_tag="RHW33_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645449.1"
                     /translation="MARTMLYKPGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            27553..28014
                     /locus_tag="RHW33_001948"
     CDS             27553..28014
                     /locus_tag="RHW33_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head DNA stabilization protein"
                     /protein_id="HDV1645450.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADCAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            28024..29442
                     /locus_tag="RHW33_001949"
     CDS             28024..29442
                     /locus_tag="RHW33_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HDV1645451.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVDGSGRVSMAHGLT
                     SQAIGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFSDSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYAGRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            29442..30143
                     /locus_tag="RHW33_001950"
     CDS             29442..30143
                     /locus_tag="RHW33_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1645452.1"
                     /translation="MADSNLNTPVIVQATRLDTSILPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILVDHEERIRQLRIEVDDHEIRIAANTAAIAELD
                     VRLTTAEGEIVTLQADVSALDGRVTTAESNISALQDDYVSKTATATQSLASPLNVTTS
                     YSVGGTKVIGARQTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVASLATGLQQARQR
                     ILALETALRLHGLID"
     gene            30143..30598
                     /locus_tag="RHW33_001951"
     CDS             30143..30598
                     /locus_tag="RHW33_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HDV1645453.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPDCRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            30601..31293
                     /locus_tag="RHW33_001952"
     CDS             30601..31293
                     /locus_tag="RHW33_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV1645454.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     KQDQQFNTIMNNLKPFTPLAEKYVGSLENLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQQWLSGQMNNYQNLANVGLGALQGQANAG
                     QTYANNMSQISQQSAALAAANANRPSAMQSAIGGGASGAIAGAGLAKLIGSSTPWGAA
                     IGGGLGLLGSLF"
     gene            31303..32718
                     /locus_tag="RHW33_001953"
     CDS             31303..32718
                     /locus_tag="RHW33_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV1645455.1"
                     /translation="MATWQQGINSGGFLAGIGMQNENAPKANDINATLGLIRENNELA
                     RSGANNVGLTALRGLAGVADIYNQEQQQKAISAFNKVHADAWASGDPSGLFKFAQENP
                     AFVAQAQQAFSGLNDQQRNDMGDLAMRANVALSQGPEAYSKFITDNKDRLNRVGANAD
                     WMIQTGIQNPEQLSHMLTTMSLGALGPEKAFAVQDKMAGREIDRGRLAETIRSNQAGE
                     ALQARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNME
                     KGIETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLR
                     IRQNALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQ
                     FNEFKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSLNTDAAPAQDSTPS
                     GEPRDQVGSYTSKSGIQFTVE"
     gene            32718..34556
                     /locus_tag="RHW33_001954"
     CDS             32718..34556
                     /locus_tag="RHW33_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HDV1645456.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGAAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANVKPGTPAYERALNQAANAVRPLLTNEATRQ"
     gene            complement(34576..34905)
                     /locus_tag="RHW33_001955"
     CDS             complement(34576..34905)
                     /locus_tag="RHW33_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645457.1"
                     /translation="MLEIVVLALGISCCVLYAGLAALKKQVKELDRSHEIDTKIARLT
                     EENKHLKNSIRALTDDNYKLSHALAKWEIVSYERMTDMIFSSYMATKSPETSGKAIIA
                     AIEKRIK"
     gene            35024..35446
                     /locus_tag="RHW33_001956"
     CDS             35024..35446
                     /locus_tag="RHW33_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645458.1"
                     /translation="MKRIIGVVAGAILLSGCATIVGDETQLVQVNSNPSGASFKVKDE
                     SGVIVAQGKTPQGVTLAKSDGSYFGKKSYQITMEKDGYEPVTLPIKANANGWYIGGNL
                     VFGGLIGWLAVDPFNGGMYTLKPKEANASLIPSTKQDQ"
     gene            complement(35460..35711)
                     /locus_tag="RHW33_001957"
     CDS             complement(35460..35711)
                     /locus_tag="RHW33_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDV1645459.1"
                     /translation="MARDDPHFNFRMPMEVREKLKFRAEANGRSMNSELLQIVQDALS
                     KPSPVTGYRDDAERLADEQSELVKKMVFDTLKDLYKKPT"
     gene            35802..35963
                     /locus_tag="RHW33_001958"
     CDS             35802..35963
                     /locus_tag="RHW33_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDV1645460.1"
                     /translation="MKGMSKMPQFNLRWPKEVLDLVRKVAEENGRSVNSEIYQRVMES
                     FKKEGRIGA"
     gene            36032..36910
                     /locus_tag="RHW33_001959"
     CDS             36032..36910
                     /locus_tag="RHW33_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HDV1645461.1"
                     /translation="MKSIAKAQNDFTIFKFGDSEIRVINKCGEPWFVAKDVCDALTLT
                     NSRKALTALDDDEKGVTLSYTLGGEQNLSIVSESGMYTLVLRCRDAVNKGSVPHKFRK
                     WVTAEVLPSIRKHGEYVKGKKTTVEERTPLRDAVNMLVGKKGLRYDDAYNMVHQRFGI
                     DSIDELSIEQIPLAVEYIHRVVLEGEFIGKQEKKANELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WMRLKNKELPPSVHNY"
     gene            37011..>38915
                     /locus_tag="RHW33_001960"
     CDS             37011..>38915
                     /locus_tag="RHW33_001960"
                     /inference="COORDINATES: protein motif:HMM:NF023640.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645462.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYMENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGAQQFY
                     FPNVLKYEPDQLRLELESSGGAGLIGGVSKPITFFGAIGDGLTNNTQAFNDAEISEFN
                     DIYIPDGVYNVNNIKLNKRYWGPGIVVINYQTEYSGSGVNDMTVSGIYNGQQDLNAIV
                     RIKEASNPDKLEFSTDNGVTWRDTIDVYNPIDDSVSPQPIVILAGGVDFFVSGLKINF
                     SSTTGHTVNDSWSFFIPSNPSVINTGSGSIVKSGSVIFNVSGNNDTNTSAGKDSLGGG
                     NVGANNTAYGWKSLHSNTTGYANTASGIQSMENNKTGKNNCAYGADSLRANVSGSDNV
                     AVGVFSLGANTTGYGNTGLGNDANRYNETGFGNTAAGVQALYHNKAGNENTAFGEYAL
                     RGGNSSLPTGTSIQYSVAVGAKAGFNALGNNNTAIGYEALYSASGSDNVGIGFDAGFK
                     VTAGAFNVFLGSNSGNVSEQATYVTNCVLLGDNTKSTGSNAVAIGSGAIAAQNTVAIG
                     NSEHELFLHYGKITPQIDNAFDIGGPYARYKTIYATTGMINTSNEMLKTKLTDIDVAE
                     KKAALEIKDNI"
BASE COUNT        10609 a         8835 c         9528 g         9943 t
ORIGIN      
        1 actctgggtg ggtacatgtt ttcccaatct ttaaaatctc cacctttacg catcatcatt
       61 agcgcagctt cgaaaccatt taattctaag caaaattcga taattccaat ttgagcaaaa
      121 tggataaggt catcaatttg gcaattaagt agttcagcag ctcgttttag ggagcaaaat
      181 tccattacag gaatactatt cctttcccaa acatttaatc gtgtattatc gctcatcacg
      241 tcacctgtat gcacatatgg taaaatgggg cgaatagcag actttactac tattagccat
      301 tttcttattt tcaaacaacg cgtagttttt tcattttata gggggcaata tatctatcag
      361 tgttagcttc aatataatta gaccaccatt gcatcatccg acgacgttca tgcatatgct
      421 gagctaagtg tacataagcg gctcgcactt catttcgttc ttgatggctc atttgccgtt
      481 ctactgcatc cctcgaccat aagcctgatt ctgtcagggc tgagcacgcc attgccctaa
      541 atccatgaag gcaaatgtcc acctgcgtgt cgtaccccat aattcttaat gacttattaa
      601 ctgtgttctc actcattggt ttgtaagggt tactatctcc ggggaaaata agttcatact
      661 caccacttat ttttttaata gcttcgagaa catcaatagc ttcattagat aatggaacat
      721 aatgaatact gcccattttt gccccacgct ctgaatattt taccccatca attctttttc
      781 tttgagcagg aatagtccac attgcatttt caaaattaat ttcctcccat cttgcgtgtc
      841 tcagttcact ggagcgaaca aaagtatgaa gagaaaataa tactgccagc ttagttaacg
      901 gcctccctgt atagctatca atttttgata ttaattcggg taatcgttct aacggaagtg
      961 cagggcgatg ctttattttg gggctggaaa ttgctcctct tagatcatgg gtaggattgt
     1021 attcaacaat tccacgctga atagcgtacc ggaaaacgct tgcaagagta gttttcactc
     1081 ttcctgttgt tgctccaatt ccctgatcac tcatcctcac caataatggc aacaggtcac
     1141 ttgtttttaa tgagtcgatg ggtttatttc cgatatatgg gaaaatatga ttttccattt
     1201 ctcttaaaat tttagtgcgg gtaatctctt tccatgaggg atggcctata gagcctttat
     1261 gccactctaa agcgattgtc tggaatgtag cagaggcggg ttctgttggc gctgcttttt
     1321 tagatactct gggatcaaca ccgtcggcta aaagttgcct tgcttcatct ctacgcttac
     1381 gggcaagtgc cagcgaaacg tctgggaaaa cgcccaaagc cagcgtagct tgtttgccgt
     1441 taaatctgta gttcattcgc catgccttgg tcccgttctt tttaactaaa agatacaggc
     1501 ttcccccatc cgtcagtttg tagtctgttt ctttgggttt agctttttca actttcttgg
     1561 gtgatagctc atttagtgcc atggagagta atctttgttg tcagttgatg gtatcggttt
     1621 atcgaaccgt gagtaccatc aacgatacca tcagaatcat gtaatgtcat gaaatcaact
     1681 gaaaccatgt tcaaggcgac aggccctgat tttgctggtt ttaagggggg aggtgaagtg
     1741 tgttgaaacg tgatgaaatg aaaaggtatt gtcccctgca gacatctact tgacgcggca
     1801 ggggattgat tggaatggtg ttttttagat gtgagaaata ttttacccgc tattttaccc
     1861 attggcgcgg cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc
     1921 tcgcccgtgg ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa
     1981 ggttttacca aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggctg
     2041 tagttcgtgt tgcatgagtg ctatctccga atagggaatc gaacctgcaa atcaggcaat
     2101 aaaaaaccgc catcaggcgg tttggtgttc tttcagttct tcaattcgaa tattggttac
     2161 gtctgcatgc gctatctgcg cccatatcat ccagtggtta tagcagtcgt tgatgtcctc
     2221 tgcttcgata accctgtcga atggctctcc attccattca cctgtgactc ggaagtacat
     2281 ttatcatctc cataaaacaa agctcgccgt agcgagttca gataaaagaa atccccgcga
     2341 atgcgaggat tgttattcat tgccgatatt tacctttatc gcgaatacat ttaccggttt
     2401 atcaccgaag tgcggatgtg tgattgtctt gatttcatat ccgtcatacg gaatatcaat
     2461 tctgcggctg gaatcgtcgc gcttcggata tccctttgtg ataatcaggc ggtcatactc
     2521 ccggaacata attcgcttat tccagtagtc attacacagg cgatactctt ccgttttctc
     2581 tccacgaatc atggcatcga agtattcacc tttgacggaa agttgcaggt tagccacggt
     2641 taacctcctg cggcggttct ggtagcggca tccagtgtgt gacgttgcgg ctctgcgttt
     2701 cgaaaaattc atcaccatca cggacgacat caaaaaactc accgtctcga tattgcgcat
     2761 aaagaacgaa tgcgccatca cataaaataa ttacgtgctg accatcatct ggcattcgct
     2821 cactacagct tatccaacca tccggagtta ccggatagtt gccgggttct ttaatgtgca
     2881 agcgaggctc accatctttt ggctcaggcc actggcgctc catgttgatc ttcaatttat
     2941 cttccatagc agcggtaatt tcagcatcgc tgatgccagc acggcgctgt gcatcccaca
     3001 acagaaactg catatcagcc cactcgctaa gatcgtctgg ttcggctgcg gcttccagtg
     3061 cctcttttga gagatgtttc agcggaccaa tggggccaac gcagccaaat gtggagtcag
     3121 accatttggc atgctcgtgg cgaatctgtt cgcgttccag tgatgccagt gcaattcgtg
     3181 ccagttccat ttgttcgcca cgagtaagtc cgttatcaag cggattttta atgaataatt
     3241 tgatacgttc tttggttata gcgctcatat cactctcctt tgatgcgaat gcctgttgca
     3301 atgctgttta tgatgctgtc agtgcatggg gtagaaagct gggcatctcc agcaattctc
     3361 atgacctcaa catctgcata tcgaataccg aggtgtatca gaccggctat acctgactta
     3421 agccgagcat tttccataaa cagatccttt gcccgctgtt tttctgcctc aagctcaacg
     3481 cgcaacttcc ctaccgttag cgcaatatcc tcattctcct ggtcgcggcg tttgatgtat
     3541 tgctggtttc tttcccgttc atccagcagt gccagaacta cttcagggtt gaaagctgcg
     3601 atataacgag cgttgttctc tgcgttgttc tgtccatcaa agccggtcca tttgataacg
     3661 tcttcacatc gtttatcacc aggtgtatgc accgcatatg taccagtacc agacgaaata
     3721 aatgcgaccc attcaccctg tgttgccagt tgcgccgcct cacgcagtgc ctgatagtca
     3781 atcttgctca ctggctgcct cctttgcgca acatcgcatt cagatatttg ttttgattca
     3841 ctgaaggaaa agaatttctc ttaagcaatt cctctctcga tggcattggc tttacgcgtt
     3901 ggcgaataat catttctgcc ggaagaatgc cgggattgta tgcaagtcct ctcatggttt
     3961 actctccacg aactggtcaa tagctatgct aagtgaaaca cctaaagtct cgatatgctg
     4021 ctgaatatcc tgtagcgtct gcgcctgaga taacaggatt tcacggttac atagctcttt
     4081 aaccagatgc tcaaacttgc tgtaataacc gatacggctt agtgtttctt tccctgcatt
     4141 atcaccttct ttgataattc ctctttcact aagaatcagg tcgtgtttgg ttccggtaat
     4201 aacgtatttg ccgaggtcga tgtttagctt cattgttttc attgttaatt cctcagtcat
     4261 tactgatagc accatagcgt gagcggtaat tacgcaggcg cgggtcaatt tcagggaagt
     4321 gtgtatatgt tgctttgcgg aatggtcgga ttgatgtctg gtaaattcgc tcgcgttctt
     4381 ctttctctgc aagccatata cagtggcgaa attccttttc ctctttcgtt tcctgcggta
     4441 gagacattat caggtcgtag ttttttctga atttatccag cacctccgat acggaattgc
     4501 cggaacagcg gcgcgggtca tccgcaccat acagaggcgc tggcatgatt aaatccttat
     4561 ttttctgaat cagaatggga tggaatcgtc gtatacagga gtgttctgct ggttactact
     4621 ttgctgctgc gggtcatttc ctgaagctgc aaatccaatc tttgcattca gtaattcaag
     4681 agtgattgat tgaccatttt gcccctgata aacatcaacc ctgatgtttt ctccggtaat
     4741 ttctacaatg ccaccttcaa caagaacact acggtagtaa tccgcttgcg ctcccggctt
     4801 ggcaaataca acggcgctgt agtttgtcca ttctttcttt tttgtctggc gatcgtaata
     4861 ctgaacgcca gcacggatgt tgaatccgat attttccccg gcctgaaact ctcttgcggg
     4921 cttgtttagt cttacagtaa tcgaatgtgc cattaagcag ccgctccttc taattcgtct
     4981 cgtctgatgt tgtaaacgtc ctgcgctttg tgctgctccg gtgtgccttc gagcatcttc
     5041 cacgctttgg cgaacgcctg tttaagctct tccacggtgt ttttctgcat tgctgcgtca
     5101 gtgaatgctt ttagaacctg ttcaggtgta ggtgatggtt ttgattgctt tgctgctgcg
     5161 ttctgctgat gtttatgctc gtctgtatct gcatctttcg catcatcaat gccgaataaa
     5221 ccattgaggc aatacttgcg tgcataagag cttgtagctc ccgtaacttg tgcagaatcc
     5281 attcctttct tgctttcttc ctctcgtgca agagcggttg ccgtatgact gttttcgcca
     5341 tcggtaatag ttgccgtggc tttcacgtaa taccgatcac caatcaacac aacttcatcg
     5401 ctgattgata aaaacaggcc attcagtaac ggcttaacac cctcaagaat gtcttcgcag
     5461 cttctgtatt tatatttgcc gaatgagtta tattgattct ttggcgcgtt cagattctcc
     5521 tgaatagctg ccagtcttgc gtaaaattct ttgctcatat gattgttctc agaatggaca
     5581 tggcccaagg aaataacgct gatttaatac ttcgactcgg gacaaattaa ggcatacccg
     5641 cattccttcg cggtcgccat tatggcgata ccagagagct ttctgcgtgt acatgcgtct
     5701 ctgtaacttg ctctccttca ctgtggttgc aagtgacatg aatatctcct tcgttgccga
     5761 ttaattcttt catcagacga atgaattctt cgtctgacca gttatctgta aaactcattt
     5821 cctgcgatac cacggaaggt tgatagctga tttcatcgct ttatttgctt caagccacat
     5881 ttttgaatca ccaataaatc tggctattac tgctttgttc tgtgcagcac gaagcatctg
     5941 gtgattgatg gctatttcat tgcgcataat aagacctcaa ctcttttcca tccgtcacgt
     6001 aatttacggg tgattcgttc aagtaaagat tcatttagtt ggaaggcacc catgcgagcg
     6061 cctcccgcga ttgcgtaaat catgggtggt tccttatgtt ggttttatta gtaggttatt
     6121 tttgttgcga atacttcgcc ttttacgatg gctgttatga tatttttagc aacatcttct
     6181 gatgcgccaa ccttgataag gtcagcaagt attttgttat ttacttcttt ccggtgagct
     6241 ttatcctttg ctctacgctc ttcttcgtcc ttgattcttt tttcttctgc tattctggct
     6301 tgctcttttg cttcagcctc gcgcctgatt cgttcagcct cctcctgtgc ttttcggcgt
     6361 tctgcttcaa ttgccgcctg cttttctctt tcagctcgtt ctgctgcctc ttttgcttcg
     6421 cgctgtgctc gttgctcggc ttcaatgcgt tcacgctctg cacgttccgc tgcggcctta
     6481 gcttctgctt ctcgccttgc tgctgcttca atttcggctt ttgcctttgc ttcggcttca
     6541 gctctggctt tctcttcagc ttctcttttt aagcgttctt catgctctcg cttttcctgc
     6601 tccgctttga gtcttgcctc ttctctttgg cggtcaaatt cgcgatccat caaaatcgct
     6661 atttcatggt cagactcaat ttgctttgcg agagcttcag ctgccgcctt agcttcttct
     6721 tcggctttaa tccgcgcctg ttcttcctca taatcagtaa gaggctggcg tgccttggct
     6781 ttcagctcat caaggcgatc acgcactgtc ttgcggttgg catcaattag ctttggaatt
     6841 tccttcagtt cagcaacaag gtctttgcca agaccatcga gatatgtttt cgtctgcgca
     6901 actttatacg ccagagaagc gatctccttt ctgccctttg ccgttgtgat atcaggcaca
     6961 aaggacataa cttcacgttc aaccttttgg agaatttctt caatctggtc ggcagactga
     7021 aatacagtca ttgcatttgc tttttcaata acaactaaat ctgttacttc actcatatat
     7081 cctccatcaa aaaaaattgc cctcacactg gagggcaaag aagatttcca ataatcagaa
     7141 caagtcggct cctgtttagt tacgagcgac attgctccgt gtattcactc gttggaatga
     7201 atacacagtg cagtgtttat tctgttgttt atgccaaaaa taaaggccac catcaggcag
     7261 ccttgttgta aatgttgcag gtatcaagta agtaattaga tggagcgcca taaattatga
     7321 attcatcgtt tgtcgggtcc atctccatct cttggcctat tgccattctt gcgtcagtgt
     7381 catcagaggc gaagcataaa acagcccacg cccccattgt tttaaaaaga actgcaattg
     7441 gctgtggttt tactgaattt gcgttagcgc gaaaatcata aatcgcactt tcatgaaatt
     7501 ccatatctca cctcaatcgt aataagctgg aattgatttt ccgcgttgct tctggcggcc
     7561 tgagcaagtc acacccattt cactgcgtgg cttgcggtag taaatacgat tctgtttact
     7621 ctcgacttct tctgccttct tgcagcgaag gcttccgagt gatgctgctt tatctgctct
     7681 gacgcaacca gagagcttta gcgcaatttt tcgcgccagt cgctgctctt gcattgcctg
     7741 ttcacgttga gcctgtctgc gtgctctgcg gcgatttctg gcgttatcgt cagccagata
     7801 tgtaatgact actgtcatgt tgacctccga tgattgactt tggcggtgac gcgccgggtg
     7861 cttatcttcc ggttgccgtc gtgcagctgc acttcacgtc accccaaagc caactactct
     7921 ttggttcccg catttcggcg ggacaatccc atcaatgtta aagagcctgc caatctgttc
     7981 cgtttggctt ccagcgtcct gctgatggct aaagaatact gtaggtattt tattgtgtaa
     8041 atacccaagg tatttatttt tgatgaaata atgataagca aatgaataca aaggatattt
     8101 attttttcgg tgtctgcttg ttcagtgctt tttatgcggg atatgtgaag tggatcccga
     8161 tagctattgc tgccgggatt ataggttagt cagcgaaggt taagacgaga attaccttaa
     8221 tgatgtctgc tacaacagac acggccatag ataaaccaaa gacgatccaa gccacagtga
     8281 tgtcttcact accatcgtat agagttccgt aatcactggt gtaaggcgta aatgtcgcgc
     8341 cttgatacaa taggtataag cttgatccat agaggataaa tgcagatatc ccttgtattg
     8401 ctatgatcac tagaatcatg aaacgagctg atctatgcgc ccaagcctgg cttatttttt
     8461 ctgatagaga tttcgcaata aaagcatgcg ctaagccgta aattgtcgag attgccaaca
     8521 tcccaaaaaa gcttgctata gcggttccaa ccataagcgc cccttgcgtg atcaaaccag
     8581 ccttagtttt gtctcaattg caacgcctat aatcttgcag tttccattga ttggcacgag
     8641 aggccatgca gggttaagtc ccttgaggta tttatttccg ccgtcgatta tcagcttctt
     8701 gaatgttgct tcgttagagt cagaaagttt tgctatgacc aagctgccat tgatcgcctc
     8761 ccttccggta tcgaaaagaa cgaatgttcc ctctggaatg cttaacccaa ccggtgccgt
     8821 cattgaatca ccttccactt taagccagaa cgcattacct tgaatatgcg cgtcagactc
     8881 aagccaaaca tctatgtctt taatggtgta tggttcgcat gcttcacacc acgagccagc
     8941 ctggatactg cttaacaccg gatacctctt tcctgctctg tattcccctg cataccttac
     9001 gttggcatcg ctcttaaggc tttctgcctg ttctgcaacc ttggcagcaa ttgactggct
     9061 aaaatcagca attgagactt gcaacaatcg tgcaaaacca gatgcaacct caacgtttag
     9121 cgcgtttctg ccattaagat aatgccctac cgctccttgg gtgataccca gttcatcagc
     9181 gattgagtat tgggttattc ccaattcttt cttttttgac tcatacaaag ccttaagccg
     9241 cttagcgtct tcgagctgtt ctgtcgtcag tgatttttta ttttccatag cttaattcta
     9301 atagctaagg tacttaaact aaaaataccc tgagtattga ttgctttgaa tacctgtagt
     9361 attctttgtt catggttaat aacggagagt gcatatgatt cgaatgacac ttgccgatta
     9421 cgccaaaatc catggacagg ctaaagcagc cagtgacttt ggtgtaatcc agtgcgctat
     9481 cagcaaggcc attctggcag gccgtaacat catggttacg gtaaagcctg atggcagtgt
     9541 gattggagag gaagttcgtc ctttcccaag caacaagaaa aacaaatagt aacaccgctc
     9601 tttaacagtc atggtcctca ttcccgccga aatgcgggaa tacaacgcgc ataatttgat
     9661 gcgcataact tcttatttgt taaggaaata cttacatatg caacttacaa gtactcgcaa
     9721 gaaagcgaat gcaattacaa gcaacatcct gaatcgaatt gctgtacgtg gtcagcgaaa
     9781 ggttgccgac gcgttaggga ttaatgaatc gcaaatttcg cgatggaaag acagcttcat
     9841 cccaaaaatg gccatgcttc tggctgttct tgaatgggga gttgttgatg atgagatggc
     9901 gagacttgct cagcaggtgg catcgattct cacaaaagaa aaacgcccag ctgttggtag
     9961 cagtctggac gcttaagcac actgtgttac gccaagtaac aggagtaatt atgccaggac
    10021 aaaccaagca agtaaacacc ggcataaagg ccggtgacaa gttcgaaacc gtatatccat
    10081 ttatcttcgt atgcacagat tatcagtctt atgacggcaa cgtgcatacc gatgagcgct
    10141 ggattggcgg ttgtcacaaa aattatgagc cagccgattg tggttatggt gatcagtgtt
    10201 tttacaccgc tgatgctgaa ggaaagagaa ttcttgaggt tctggcagtg gtggaaatgc
    10261 caggcaactg gcagcgcaga atcatttatt cgtgccacct catagagcct gaaggcaagg
    10321 agaggaaagg aaggaaggct tacacagtca ccgaatcaag gtttttaaaa atgtctactg
    10381 gttacttcgc tgattacgaa gtagaggagg cttaatgaca aaacaactca gtccttacca
    10441 ggacaaaatt cacaaacaca tactacgtga tcgcttcctg tccagcttca agcagcctgg
    10501 tcgattccgg gctgagttgg aaaaagtgaa gctgatgcag aagtagaaag gtcatgagta
    10561 atcttgcaac cgtaacacat ttaaggtctt cacaacggcc tgtggagcgt cgtgtggcag
    10621 aagttgaaga tggttatacc cgtcttgcaa atgccctgta tgaagagctt atcggcgcag
    10681 atttaacgaa aaatcagagc aaggttgccc acgccatatg ccgtaaaaca tacggctacg
    10741 gtaaaaagat ggatcgcatc tctgatagtc agttagctca aattaccagg ctgccaagac
    10801 agaaggtaaa caaggccaag aatgagctta tcgcgatgaa ggtaatcctt cgcgaaggcc
    10861 agcaaatcgg gcctaacaag aacatcgagg aatggcaaat cgaagggtgt cactactctg
    10921 gtgataatgt cactgcattg gtgacaaaaa gtgtcaccaa aacggtgaca gcgctgtcac
    10981 caaaacaggg acacacaaaa gaaactatta caaaagaaaa aagaaataat aaaaacacta
    11041 tgtccgaaag tgttcggacg gagtgtgaaa aatcacctga ccgtcacgaa gaaaccgaca
    11101 aggcattcga ggaaatattc tggtgtgcag gcatgcggaa agctgggaag aaaaacgcgg
    11161 cttcggcatt cagaacacag ttcagggaat ggcgtaaaac tacaaggggt acggcaagcg
    11221 agtttgccac gatgctggca gaagacatcg catgcaggaa tggtaagcag ttcggattcg
    11281 acagattgtt accatcgagc tacctgaacg gtcagcgctg gaacgacgaa aagccagaaa
    11341 ccattcaacc acaatccaaa ccatcatccg caatcaccgt atcgaaaact ggctacgtgt
    11401 ttttcgacag gtgaaccatg aaatcaaaaa tcaaatcgct actggtcgct ggttataacc
    11461 acggctggtt aagtatttcg tttgtcgatt tctggtttaa aaatctcaat ctgagggaat
    11521 catgatgagc cttgaagttg gagaatatat acctggttat gagggtaaat actctgtaac
    11581 caaagacggt cgtgtttatt cacatttaag aggcaggttt ttgaatccat ctccggataa
    11641 caagggttat ctaagagtca ctttgtgttc cgatgggatt cagaagacgt taaaagttca
    11701 tcgtcttgta gctcaattat ttatcccaaa tccgtattgc aagccagaag taaatcatat
    11761 caacggcgac aaatcggata acgccatttg gaacttggaa tggtgtacct gctcagagaa
    11821 tttaaaacat gcttttagca tcggactgaa aagcgcaaag ggagttttta atggtagggc
    11881 aaaactatct gaaactgatg tgattgatat caggaattct gttggtatga aactaagcga
    11941 actatccact aaatacggag tgagtgaaat gcaaatttcc acaatccgca gaggaaacgc
    12001 atggaggcac ctttgagtcc atcagaactt agcgacctgc tttgggcgca ggttgacagg
    12061 gtggctccgc acctgttgcc aaacggcaag aaagaggggc atgagtgggt tgccggtaac
    12121 gtcaacggcg acaagggaaa cagccttaag gtcaacctta gcggcaagaa aaaatgggct
    12181 gatttcgccg agggagacgg cggtgacatg cttgatttgt ggatggcatg tcgtggaatt
    12241 aacctgcatc aggctatgca ggaagcgaaa gcatttctcg gtatcaagga tgacgatcac
    12301 catttcgatg ccaaacgtga gaagaaattc tccagacctg atcgcaagaa aatcgcccgc
    12361 tacgttacca gaacagaatc ccatcttgag tacctgcaat cgcgtggcat atcgccagaa
    12421 gtcgtaaagc gctacgaggt tgtcagcggc aaggtgtgga atggagaacg agaactggat
    12481 gctctggttc ttccgtacaa acgcgatggt gagttgttgc aggtcaagcg aatcagcact
    12541 gagcgcccgg acgggaagaa agtcattatg gcagaaggtg attgcgaacc ttgtctgttc
    12601 ggatggcagg ctctggacgc tggcgtgagg gcggttgtgc tttgcgaagg cgaaattgat
    12661 tgtatgagct atgcgcaata cggcatctcg gcgttatccg tgccgtttgg tggagggaaa
    12721 ggcgctaagc aacagtggat tgagtttgag tatcacaacc tcgacaggtt tgaggaaata
    12781 ttcatctcga tggacgttga tgatgttggt cgtgaagccg caagggaaat cgcaagccga
    12841 ctcggtgaac atcgttgccg tcttgttact ctgccgtaca aagacatcaa cgaatgcctg
    12901 atgaacggtg ttaccgagga tgaaatctgg cagtacatcg gcacggcatc ctacttcgat
    12961 cctgaagaac tctacagcgc gcgagagttt taccaggaca ctatcaacgc tttctacggc
    13021 aagcagcagt atctgtttaa tccaccgtgg gaatctctgg cagataaatt ccagttccgt
    13081 gaggccgagt tgacgctggt caatggtgtg aacggtcacg gaaaaacgga ggttgtcggg
    13141 catatggcac ttgaggcaat gcgtcagggt gtgaagacgt gcatcgcgtc acttgagctg
    13201 aagccaggca ttctccttaa acgacttacc cgtcaggcga cgtgctgcaa gatgccgcca
    13261 gtgctggaaa ttgactctgc atttaaattt tatgacgaaa gactttgggt gtttggtctg
    13321 accggaacgg cgaaagccga caggctgatc gaaatattcg actacgctcg ccgccgatac
    13381 ggcatccagt tattcatcat cgacagcctg atgaaatgtg gcataggcga cgatgactat
    13441 aacgggcaga aggcgtttgt tgactcgatt tgcgatttca aaaacaaaac aaactcccac
    13501 gtcattctcg ttactcactc gcgaaaaggt gacagcgaag aaaaaccaac cgggaaaatg
    13561 gacgtaaaag gctctggagc gataacagac ctgacagaca accttttcat catctggcgt
    13621 aacaaggctc gcgagagagc gttacagaga gttcagagtg gtgaaaggat gtcagagaag
    13681 gacgaacagc tactggcatc tccggcatct gttttgatgc ttgaaaagca acgtaacggc
    13741 gaaggttggg aaggtggtgt cccgttgttc cttgacgagc aatcgcacca gttcctgcaa
    13801 cttgaatcag gatcgccata tagctacatc gccaatatgc cgaaatcgga atatgacgag
    13861 gcgtggcgac aggaaaacgt gacggagtat taaatgacca tctacatcac tgagctaata
    13921 acaggcctgc tggtaatcgc aggccttttt atttggggga gagtaaatcg tggctgagtt
    13981 tatgctcgtc gcactcaaat gcgttggcgt tggatggatt cttctgacgt tttttattgt
    14041 tctgcatagc tacattcgtc ttgtgaatga cggtaaagac ccatggtata cgttgtttgg
    14101 cgctgcattt gtctgggtga ttatcggtgt tatgcctgtc gctgtagcaa aaatggcgtg
    14161 gcgttttgtg agttgaactg agggtaagta tcgatggacg aatcaagaaa gcagtttgag
    14221 gaatacgttg ccaaaaaatt gagattacca ttcgagatga taaccgaggc aagaaatggt
    14281 gataggtact tcgcattttc aagcatggat attcgtcact ccttaaatga gtggtggact
    14341 ttatggcagg catcgcgagc agctattgaa attgagttgc ctgtatggtt tgtcagtgat
    14401 gctattgctg tgtacgaccg tgacgatatc gatgaatcca tccgcgctgc tggaatcaaa
    14461 gtgaaggagt gagcatgagt cgacgaagta gctttttggg gtttgtaata ttcctgtcct
    14521 gcactggtta catcgtaatc tggtcaattt cgaacattga ccgtggcgtg gcatatctca
    14581 ttgtaatgtt ctttcctttg tttcttgggt ggtacgccgc aaggttgctg gaagaatggg
    14641 gttacaggca taaaaaataa aggagtgttc agtgaagcaa acaatattcc tccgaactaa
    14701 gcaacaacag caagccgcaa taaatgccat cctcgaaact cctctcgata aagacaagcc
    14761 agtcaccatc cgcattactg actacaagcg caaccttgac cagaacgcaa aatttcacgc
    14821 gatgctggcg gatatcgctt gtcaggttca atggtgcggc aaatggttaa aaccagaaca
    14881 atggaaggtt ttgttgataa gcggtcatgc agtggcaaca aagcaggaag ctgatgtttt
    14941 gcccggcctt gaaggcgaat atgtcaacat tcgcgaaagc agcgcgcaga tgagcgtgaa
    15001 gcgtatggca agtctgatcg agtacacaac agcctgggct attggtcagg gtgtcagatt
    15061 taccgacagg aggtacgaat gagacgacag cgacgaagtt tcaccgacat catctgcgaa
    15121 aactgcaaat accttccaac gaaacgctcc agaaataaac gcaagccaat cccaaaagaa
    15181 tctgacgtaa aaaccttcaa ctacacggct cacctgtggg atatccggtg gcttagagaa
    15241 cgtgcgagga aaacaaggtg attgacccaa atcgaagtta cgaacaagaa agcgtcgagc
    15301 gggctttaac gtgcgctaac tgcggtcaga agctgcatgt gcttgaagtt cacgtgtgct
    15361 ccgattgctg cgcagagctg atgagcgatc cgaatagctc aatgtacgag gaagaagacg
    15421 atgaatgagt taataaatgg caatgccatc aaaatgacaa gcattgaaat cgctgagttg
    15481 gtagaaagcc gccatagcaa tgtaaaagta tccatagata gattggtgaa acgtggcgtt
    15541 atcaagcctc ctgcattgca gcacactaac ataatcaatg atttaggtgt tattaccggg
    15601 aagcgtgatt tctacgtctt cgaaggcgaa caaggaaagc gagacagcat tattgtcgtc
    15661 gcccagttgt cgccagagtt caccgctcgc cttgttgacc gctggcgaga gcttgaaggg
    15721 gcaaccgcga aaatcccaca aaccttctct gaagcattgc gcctcgcggc tgaccttgaa
    15781 gaccagaagg ctgaactgga gaaacagctt gctctcgcag cacctaaagt tgattttgcc
    15841 gatcgcgttg gcgaggccag tggaattttg attggaaact ttgcaaaggt tgttggtatt
    15901 ggtccaaaca aactgtttgc gtggatgcgc gatcacaaaa tccttattgc ttcaggttcc
    15961 cggcgcaatg tgccaatgca ggaatatatg gatcgcggct atttcacagt gaaagaaaca
    16021 gcggtcaaca caaatcacgg aatacagata tcgttcacta caaaaatcac cggacgtggc
    16081 caacagtggc tgaccagaaa gctgctcgat aacggaatgc tgaaagtaac aggggaggct
    16141 gcttaatggc taacctacgc aaagaagcgc gcggaagaga atgccaggta cgtatttacg
    16201 gcgtatgcaa tggcaaccct gaaactacag ttctggcaca ttaccggatg gctggaattt
    16261 gcggaacggg gatgaagcct gacgacctga tcggtgcatg ggcttgtagt gactgccacg
    16321 cggagatcga ccgacgcacc cataacctcg acaacaaaga cgccagactt taccacctcg
    16381 aaggcgtgat caggacgcag gcgatactgc tgaaggaggg gaagattaag tcatgaacga
    16441 atatcagttt gtgcttccat acccgccgtc ggtgaacacc tactggcgaa gacggggaag
    16501 ccaatactac atcagcgata aaggccagaa ataccgaaaa gacgttcagc aaatcatccg
    16561 ccaactcaag ttagacattt tcaccaaatc acgactccgc atcaaagtca tcgcagacgc
    16621 tccagactcc cgccgccgcg acctcgacaa catcctgaaa ggtttactcg actcccttat
    16681 ccacgccgga tttgcggaag acgacgagca attcgatgac attcgcgtaa ttcgtggtgt
    16741 gaaagtacca ggtggaaggc ttggaataaa aatcaccgaa ctggagaacg catgaacgcc
    16801 acaattcaaa cgataccaga gcttcttatc cagacacgag gcaatcagac cgaagtggcg
    16861 aggatgcttt cctgcgcaag aggaacagtg ctcaagtaca accgagacag caaaggcgag
    16921 cgtcacgtaa tagttaacgg cgtcctgatg gtcaaacagg gcaagagggg aagaccatga
    16981 gactcgaaag tgtagctaaa tttcattcgc caaaaagccc gatgatgagc gactcaccac
    17041 gggccacggc ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc
    17101 aatcacaagc cggattcgga atggctgcat tctgcggtaa gcatgaactc agccagaacg
    17161 acaaacaaaa ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc
    17221 gtggtgtggc aaagctcgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat
    17281 tcgcttatgc ggattattgc cgtagtgctg cgacgccggg cgcaagatgc agagattgcc
    17341 acggtactgg ccgggccgtt gatattacca aaacagagca gttggggaga gttgttgaga
    17401 aagaatgcgg aagatgcaaa ggtgtcggct attcaagaat gccagcaagc gccgcatatc
    17461 gcgctgtaac gatgctaatc ccaaacctta cccaaccaac ctggtcacgc actgttaagc
    17521 cactgtatga cgctctggtg gtgcaatgcc acaaggaaga gtcaatcgca gacaacattt
    17581 tgaatgcggt cacacgttag cggcatgatt gccacggatg gcaacatctt tacggcatga
    17641 tattgacttt ttgaataaag ttgggtaaat ttgacccaaa gatggataaa tgcactcgtt
    17701 aaataaagcc ctgagttaat agctcggggc tttttgcgtt ttaatcacga cctttctgaa
    17761 agcacatcaa accaaatacc agacagacca aaataatcac cttatccgct gtggctacgg
    17821 tgcggtgtgc tttgcataaa agaaaaccag cgcaatggct ggcttcgtga aagcgggtgg
    17881 catgaggttg cgctaacaac ctcctgccgt tttgcccgtg catatcggtc acgaacaaat
    17941 ctgattacta aacacagtag cctggatttg ttctatcagt aatcgacctt attcctaatt
    18001 aaatagagca aatcccctta ttgggggtaa gacatgaaga tgccagaaaa acatgacctg
    18061 ttagccgcca ttctcgcggc aaaggaacaa ggcatcgggg caatccttgc gtttgcaatg
    18121 gcgtaccttc gcggcagata taatggcggt gcgtttacaa aaacagtaat cgacgcaacg
    18181 atgtgcgcca ttatcgcctg gttcattcgt gaccttctcg acttcgccgg actaagtagc
    18241 aatctcgctt atataacgag cgtgttcatc ggctacatcg gtactgactc gattggttcg
    18301 cttatcaaac gcttcgctgc taaaaaagcc ggagtagaag atggtggaaa tcaataatca
    18361 acgtaaggcg ttcctcgata tgctggcgtg gtcagaggga acagataacg gacgacagaa
    18421 aaccagaaat catggttatg acgtcattgt tggcggagag ctattcactg attactccga
    18481 tcaccctcgc aaacttgtca cgctaaaccc caaactcaaa tcaacagcag ccggacgtta
    18541 ccagcttctt tcccgttggt gggatgccta tcgtaagcag cttggcctga aagacttctc
    18601 tccgaaaagc caggacgctg tggcactgca acagattaaa gagcgtggcg ctttgccgat
    18661 gattgatcgc ggtgatatcc gtcaggcaat cgaccgttgc agcaatatct gggcttcact
    18721 gccgggcgct ggttatggtc agtttgagca taaggctgat agcctgattg caaaattcaa
    18781 agaagcaggc ggaacggtca gagagattga ggtatgagca gattaaccgc gattatctcc
    18841 gctctggtta tctgcgtcat cgcctgcctg tcgtgggcgg tcaatcatta ccgtgataac
    18901 gccatcgcct acaaagagca gcgtgataag gccacatcca tcatcgctga catgcagaag
    18961 cgtcaacgtg atgtagcaga actcgacgcc agatacacaa aggagcttgc tgatgctaac
    19021 gcgactatcg aaagtctccg tgctgatgtt tctgctggtc gtaagcgcct gcaagtcgcc
    19081 gccacctgtg caaagtcaac gaccgaagcc agcagcatgg gcgatggaga aagcccaaga
    19141 cttacagcag atgctgaact caattattac cgtctccgaa gtggaataga caggataacc
    19201 gcgcaggtta actacctgca ggaatacatc aggacgcaat gccttcgatg atagcgataa
    19261 ttttactcat catccttcac atctggctct gtagacaggg tggcgatcac ttctggagtg
    19321 aatccagatt aaacatctca ttgctgatgc ttgatattga gcatctggcg cgcggtaagg
    19381 ggctgcgttg agataagagc cagtcattac aaataccagg atttagcctc gcatttgcgg
    19441 ggctttttta catctgcagt aaaccgcgca tcgcagcgcg taacaatccc gagtctttca
    19501 gaaagctgag cctgagaact gccgtatatg gtggcgacca tctcggggcg gcttttctgt
    19561 gcgaacaggc tcactttcta aaaggtaaag acgctatgaa tatcgttcca ctgaattaca
    19621 aaggcgaacc tatccgcttc aatactgatg gctggattaa tgccactgat attgcaaaac
    19681 gtttcgggaa gcgtctggat cactggttgt ccaacgctga aactctcgaa tacgttagag
    19741 ctctggatga ggtttattca ggtgaaccat cgaaaattct acatacccgt gattccgggt
    19801 atgtaaaaac aagcaaggca cgaaaggaca ggggcggcgg aacatggctg catccaaagt
    19861 tatcagttgc ctttgcaaga tggtgcgatc cgaaattctc cgtatggtgc gacctgcaca
    19921 ttgatagtct gcttcgcggt gaactgactg agcagcagaa atatgagcaa gcatgtcgca
    19981 ttcgtgatga ccggaaatca aaagccagca atggggcaag agagatggct cgctggcgat
    20041 gggataagcc ggttattgaa gcaaatgttg agtactggcg cgagcaactg cagttgactc
    20101 tcgatatcgc gtgctgatgg caaacgcaaa actgcgttat cggaaaaatc aaagcattac
    20161 gagaactgag caacggctat ccattacaaa gcccatctgc gggtgggctt gataatgggg
    20221 atgaatatcg caatttttat tgtggttgac ataattagct gatttgcaat gattattttg
    20281 ctgttggtat aattaatcct ctactacgag agggatgact atgctgacaa taattttgtt
    20341 tggtgctggc tggaaaggtg atgtttgggc tctcgagagc tacagtcccg ttattaaggc
    20401 tcctgatcgg acatttaggc ttctgaaaag ctctgggggt gccccgatct tcgaggatgc
    20461 tgagtttgat gttttcgaat atcagcttgg tgatgaaatt tatctggttg gctgtcatgg
    20521 gaataagcct agtgacgatg tgattatgtc acacatccag catggtgacc ctaagccgaa
    20581 accatacaaa acactgtaac cgcctccggg cggtttttta ttgccatcac aaaagccatt
    20641 ccttgcagaa tggctttgat aatggcttat accctacacg ggataactta actgatatcc
    20701 cttttaacgg ataaacggag ccaacaatgg cagagattat tcccatgact gaagaacaga
    20761 aattccagtt agagatttac aaactggtca tgaaccagaa cgcaaccgca gaagaagcat
    20821 ttcagttcat tggcactgac gagctgaagc ttgagttatt caaaattcac ttccagtcag
    20881 gcggcgctaa ttcagatatc acgacccgca ctatcgaagc ggtgcgtaaa tcgaaggaag
    20941 cgttagacct gttcactacc ggagcataaa catggcaact caaggtttcg acaacccatc
    21001 caaattccgc gatgaatggg ataagcaagc agaagggaaa taatcaatat ggcgactgag
    21061 aaaaagaacg tcggtcgccc ttcggattac ctgccggagg tggctgatga tatctgtgcg
    21121 ctgcttgcct ccggggaaag tctggttaag gtttgcaagc gccccggcat gccagcaaag
    21181 gctactgtat ttcgctggct gtcagagcat gacgaattta gagacaagta cgcgaaggca
    21241 actgaggcac gagctgattc tattttcgaa gagatattcg aaattgctga caatgcgatt
    21301 ccagatgctg ctgaggtggc aaaggcaaga cttcgcgttg atacccgcaa atgggcgctg
    21361 gcccgaatga atccacgtaa gtatggcgac aaggtaacta atgagcttgt cggcaaagac
    21421 ggcggcgcaa tccagattga aacatcaccg atgagcactc tattcggaaa atgacctcga
    21481 ttaatcctat ctttgaaccg ttcattgagg cgcatcgcca caaagtcgcc aaaggcggtc
    21541 gaggtagcgg taagtcatgg gcaattgcga ggctgcttgt tgaagcggcg cgtcgtcagc
    21601 ctgtgcgtat tctctgcgct cgtgagctgc aaaacagtat cagcgattcg gtaatccggt
    21661 tgcttgaaga taccatcgag cgtgaagggt attcggctga gtttgaaatt cagcgttcaa
    21721 tgattcgtca tctcggaacg aatgctgagt tcatgttcta cggcatcaaa aacaacccga
    21781 cgaagattaa atcgctagaa ggcattgata tctgctgggt ggaagaagcg gaagcggtaa
    21841 cgaaggaatc atgggatatc ctgataccaa ccatccgtaa gccgttctct gaaatatggg
    21901 tgagctttaa cccgaagaac atactcgacg atacctatca gcggttcgtc gtaaatcctc
    21961 ctgatgatat ttgcctgctg acggtgaact acaccgacaa cccgcacttt cctgaagttc
    22021 tccgtctgga gatggaagag tgtaaacgca gaaatccgac actgtatcgt cacatctggc
    22081 ttggtgagcc ggtaagcgca agtgatatgg caatcatcaa acgtgaatgg cttgaagctg
    22141 caaccgatgc gcacaagaaa ctcggatgga aagcgaaagg cgctgttgtt tctgcacatg
    22201 acccgtcaga tacagggcca gatgctaaag gttatgcatc gcgtcacggt tcggtagtta
    22261 agcgcattgc cgaaggtctg ctgatggaca tcaacgaggg tgctgactgg gctacttcgc
    22321 tggctattaa agacggcgct gaccattacc tgtgggatgg tgatggtgtt ggtgccgggc
    22381 tacgcagaca gacaacggaa gcgttctccg gtaagaaaat caccgccacg atgttcaagg
    22441 gcagcgaatc gccattcgat gaagatgcgc catatcaggc cggagcatgg gctgatgaag
    22501 tcgtacaggg cgacaatgtt cgcactattg gcgatgtatt ccgcaataag cgagcgcaat
    22561 tctattacgc gctggctgac aggctgtatc tgacatatcg ggcggttgtt cacggtgagt
    22621 atgcagaccc agacgacatg ctgagtttcg acaaagaagc gataggcgag aagatgctgg
    22681 agaagctgtt tgcagaactg acgcagattc agcgcaaatt caataataac gggaagctgg
    22741 agcttatgac taaggtcgaa atgaagcaga agctcggtat tccatctcct aacctggctg
    22801 atgcgctgat gatgtgtatg cattgcccgg agtcggctgc gcaacccgac tattccagtt
    22861 actcaattcc ttgtggtgta ggttgatatg gcagaaaaaa agatgaatga ctggcatcgc
    22921 aaggtgctgt gcaactttga taatgcctgg tcagcaacgc aggatatgcg tgagcagatt
    22981 attgaggctc aacgtttcgt ccgggtatcc ggcgcacagt gggaaggcag cacaaacgct
    23041 ggttactcat ttgatgaagg caggtttgag cattacccgc gctttgaact gaataagatt
    23101 gcccgtgaat gtgatcgcat cattggcgag tatcgacaga atcgcatcag cgttaaattc
    23161 aggccgaagg acgataaggc atcggaagcg ttagccgaaa agatgaacgg caaattccgc
    23221 gctgactatc aggaaacatc aggtggcgaa gcgtgtgata acgcatttga tgatgctgta
    23281 acgggcggat tcggttgttt ccgcatgtgt gccgattacg aagatgaaat ggacccaagt
    23341 aacgagcagc gacgcatcag ccttcttcct gtttacgacc cggcgacatg cgtcttcttc
    23401 gatcaggaca gcaaacaata tgaccgctct gatgctatgt gggctatgga aatgttctcc
    23461 atgacgccta aagcgttcga ggctgaatac cctgattcca ttgcggcaag cctttctcgg
    23521 gatgacactg gtactcagta tgactggtca acgcctgacg ccatctatgt tggacgttac
    23581 tacgaagttc gcatagagaa ggtgaagctc acggcgtggc gcaaccctgt tagcggagaa
    23641 acggcaatct atgatgaaga gcaaatcaaa gatattgtcg acgagctgac cgatggtgca
    23701 ttcgaactga ttggcgagcg aacggtgaag aagcgccgcg tttattgcgg tcttctgtct
    23761 ggcgctgaat ggctggaaga accgaagcgt attccgggtg aacatattcc tctcatcccg
    23821 gtatatgggc gtcgctcatt tgttgataat caggagcgaa tcgaaggcca cgcagcaaaa
    23881 gcgatggatg cacagcgtct tgagaacctg atggtttcca tgattgcaga taacgctact
    23941 caggctggcg gtgatggcat tcctgtagtt gatgttgaca tgattcctgg tcctctcgcc
    24001 actcattggg cggagcgcaa caaaaagcgc ccggcgttcc tgccgatggt cagtctgaaa
    24061 aacaaaaacg gagatattac tgcgcaggct caggtcagca gttatacgcc tccgacacaa
    24121 atgcctccag ctcttgccgg gctattgcag tacaccggaa cggctattca gcaaattaca
    24181 ggtgcgtcgc agcttgagaa catgccgagc aacgtcgcta ctgataccgt tgatagcatt
    24241 tttaaccgga tggacacgca gtcctatatc tacatggaca acatggctaa atccatgcgt
    24301 cgcgctggcg ttgtgtggct ttctatggcg cgtgaggtct atggcagtga tacgccgatg
    24361 cgtatcgtta atgaggacgg cagcgatgac gtggcgctga tgactggtga agtggttgac
    24421 cgtcagacag ggcaggttat cgcgcttaac gacctttcgc agggtaacta tgaagtgact
    24481 gtcgatgttg gtcagtcgtt cgctactcgc cgtgatgcaa cggttaagtc gttactttcc
    24541 atgctggcac ttatcccgcc cggaacgccg aagcacgacc ttgtatcgtc gatgattctc
    24601 gacaatatgg acggcgaagg gatggacgac ctgaaagaat acaaccgcaa tcagttgctt
    24661 ctgtctggag ttatcaagcc gagaacacca gaagaacagc agatggttga gcaggcgaaa
    24721 caacaacagg ccagtcagcc agatccggct atggttgctg cgcaaggtca gcttcttgct
    24781 ggtcaggctg aattgcagaa agcgcagaac gaacaggcag ccattcaggt taaagcattc
    24841 caggcacaga ctgatgctca ggttgcagcg gcaaatgttg tgaaaatcct cgcatctgcc
    24901 gatagccagc agaaatctga tatccgcgag gctctgaaac tgctcggaca gttccagcaa
    24961 cagcaaggag acaatgcccg tgctgatgca gagcttgtcc tgaaaagtca ggcacagggc
    25021 catgcgcagc gcatggacat cagcagcatc ctgaaaaaat caactcagca acaaccacag
    25081 cagtaattaa cccataacgt gcaatggctg tctttatgag gcctggcacc ctattgcctt
    25141 ccgatgggct gaacatcgag taaacagggg taacaaatgg accagatggc agaaaacaca
    25201 ccagaagttg aaatcgaaac cgacgcgtca gagcagattc ctgatgatgt cgaactggct
    25261 gaagaagtcg aaacagaaga tggcagtgag tcctccggca atgatgcaga ggaagctact
    25321 gaaactgatg acgacgaatc agaacaggaa ttctactttg gtgacgaaaa gctggattcg
    25381 ccaaccagcg aagatggcgc agagcatgga ctggtaaaac acctgcgcaa gacgattaaa
    25441 gagaaagacc gtgagctgaa agagctgatg cgtcagtatc agaaacccgt cgagcagcag
    25501 ccggtaatca ctcaaccacc gcgaatgcca aaactggatg atgaggacat cggtttcgat
    25561 gaagaaatct accagcaacg catggctaag tgggcagagg ataacggcaa gtaccagcaa
    25621 caggagatgg cccgcaagca gaaggagcag gagcttcagg ctgcttatca agagcgatta
    25681 tccaaatatc agcaacgtgt taaggctctc aaggttcctg gctatcagga agcagaacag
    25741 gccgtactcg aggaaatccc catcgagaca caaaacgcga tcctgtttga gtcagagaag
    25801 ccggaaatcg ttgttctggc actcggtcgc aatgctgaac tgcgcaagca actggcagaa
    25861 gctaccaacc ccgtagcaat tggtcgtctg ctggaacgta tcgaatctaa ggccagaatc
    25921 atgccaaaag caaaaaccac ggccgccaca accccgacag ttaaggggag caacggcgca
    25981 gtaatcaaca acctcgacaa attgaaagcc aaggcgctgg aaactggtga ctggacgccg
    26041 tatttcgccg ctaaaaaggc aaaaaaataa cctatcggag cattaagcat ggctaaccaa
    26101 ttagcaaaag accttgaaat catgttcgaa aactacgttg aaggctttga ggccgcctgc
    26161 gtagtttccc gtaacgctaa aaaattccgt cccggtgata cagcaatgca gcgagcaggt
    26221 gatgttctgt atcgtccgca gcattaccac atgaacattg aggaaggcct agatctcagc
    26281 ggcaaagcgc caacagcact ggttcagcgc cttgttcctt ctgtgttcaa ggagccgaaa
    26341 aacattctgt acactctgga tgcgcgtgaa atgcgtgacc cggaacataa aactgaagct
    26401 ggtcgcgccg caggtatgcg ccttgctgca cagattgact ctgacctgat ttccatggtc
    26461 acgcagcgtg ctactaacgt gatcacaatg gctgactcaa ccactggttc acagggccgt
    26521 gatttgtgga actgtgcggc aggtattgat gccaccatga cggcgattgg tgtacctcag
    26581 ggtatcaacc gtcgctcttt ctggaacccc ttcaactaca aagaccttgc tggcgagctt
    26641 ggtcaccgtg cctatgctca gggcgcaacc ctgacagcat acgaaaaagc gcaaatccct
    26701 ccggttgcgt ccttcgatag ctacaagacc gatatttctg gtcgtgttcc gaagggtaca
    26761 gcaacttcca ttacgctggc agcagcacct gcgcacaagg ttgaagcgaa agatgctaac
    26821 gatatgccag tggataaccg acaggggacc attacggtat ctgctgaagg tttgcaggtt
    26881 ggcgatgcgt ttaccatcgc aggggtgaat tctgtacacc agatcaccaa agataccacc
    26941 gggcagccgc aggtattccg cgttctggca gtaagcggaa cgacagtaac tatctccccg
    27001 aaaattctgc cgcctgacaa cgcggatgtc gccagccgtc catatgcaaa cgttgatgct
    27061 aatgcggcaa gtagcgcagc aatcaccatt ctcaacaaaa atgccgcacc ggctaacctg
    27121 ttctgggctg atggttctgt tgaactgatg tacggcaaac tggcgttccc aactggtcag
    27181 ggtccacagg taatgacggc aaccaccgag cagggcgcta cgctgatcat gtcttacgcc
    27241 ttcgaccaca tcaaaggcgt aaccactgcg cgtttcacca ctctgtacgg ttgctctgta
    27301 ctggttcctg aatatacggg catcgttatt gccgggcagt aattttggtg gggcttcggc
    27361 cccattttta ttgggagaag acaatggcac gaacaatgct ctataagcct ggcaacatga
    27421 tcacctgtgg tcagtttgct gtcgattaca tcattgttga tgacgaagaa gttaaatctc
    27481 acctgaaaaa aggttgggta aaaactcctg aagaaaccgc aacgaagcaa aaagtggcta
    27541 aggcggaaga agatggcgaa aacgaagggt gatctcgttc taaaggcttt acgaaaagcc
    27601 gggctgtatt ccaatgccac gttgacagat gctgaccctc aggcaattga agatgccatt
    27661 aatgacctcg aagacatgat ggcagcatgg caggcgaaag gtatcgagct tgggtatcag
    27721 tttgctgata cagaaaacgg catcatgccg ttacctgacg atgattcagg tatccctgca
    27781 tgggcaaatg atggcgtcgc tttgaaactc gctgtgcaag tgtgcatgga taacgtcatt
    27841 cagccgtcag acgctctcct tactgctgct gactgtgcat atcagacaat ctgtatcgct
    27901 ttaaccaaaa taccaccact tgagcggcga aatgacatgc ctcgcggtag tggtaacaaa
    27961 agcgcgttta cgtggaatcg gttttacatc gagaaagatg atccgagtac gtgaggtgaa
    28021 taaatgccga ttcagcaact tccgcttatg aaaggtgtcg gcaaagactt tcgaaacgcc
    28081 gactatatcg actatctgcc agtgaatatg ttggctacac ccaaagaaat cctcaacagc
    28141 agcggatatc ttcgctcatt cccgggcatt gccaaacgtt ctgatgtgaa cggcgtatcg
    28201 cgaggcgtcg agtacaacat ggcgcagaat gctgtttatc gtgtgtgtgg cggcaagctc
    28261 tacaaaggcg aaagtgaagt cggtgacgtt gacggaagtg gtcgtgtatc catggcgcat
    28321 ggtctgacat cacaggcgat aggcgttaat ggtcaactgg tcgagtatcg ctatgatggc
    28381 acggttaaaa ccgtctcaaa ctggcctaca gacagcggat tcacacagta cgagttaggt
    28441 tcagttcgcg acattacgcg cttacgtggg cgttatgcgt ggtcaaaaga cggaactgat
    28501 tcatggttta tcactgacct tgaagacgaa tcgcaccctg accgatacag tgcacaatat
    28561 cgcgcagaat cgcagccgga cggcatcatc ggtatcggca catggcgaga cttcatcgtc
    28621 tgctttggtt catcgacgat tgaatatttc tccctgactg gtgcaaccac agttggtgct
    28681 gctttgtatg tcgcccagcc atcgctgatg gtgcagaaag gcattgccgg gacttactgc
    28741 aaaacgccat tctctgattc atatgcgttc atcagcaatc cggcaacagg tgcgccgtct
    28801 gtgtacatca tcggctccgg tcaggtgtca ccaatcgcca gtgcgagcat tgagaaaatc
    28861 ctccgctcct acactgctga tgaactggct gatggcgtga tggaatcgtt gcggtttgat
    28921 gctcatgagt tgctgattat ccatcttccg cgtcacgtcc tcgtgtacga tgcatcttca
    28981 agcgccaatg gtccgcaatg gtgtgtgctg aaaacaggac tgtatgatga tgtgtaccgc
    29041 gctatcgact tcatttacga aggcaatcag ataacgtgcg gcgataagct ggaatccgtg
    29101 accgggaaac tgcaattcga catcagcagc cagtacgaca agcaacagga acacctgctg
    29161 tttactccac tgttcaaagc ggataacgcc agagtgttcg accttgaggt tgaatcgtca
    29221 actggcgttg cgcagtatgc tggccgcctt tttctctctg caaccactga cggcatcaat
    29281 tacgggcgtg agcagatgat tgagcagaat gaaccgttcg tttacgacaa acgcgttttg
    29341 tggaagcgag tagggcgcat caggaaaaat gtcggcttca aattgcgcgt tatcacgaag
    29401 tcacctgtca ctctgtctgg ctgccagata aggattgagt aatggcggat tcgaatctca
    29461 atacacctgt tattgttcag gcgacgcggc tcgatacatc aatccttcca cgcaatatct
    29521 tctcgcagtc atatctgctt tacgttatcg cacagggtac tgatgttggt aacgtggcga
    29581 acaaggccaa cgaggccgga cagggcgctt atgacgcaca agtcaggaac gatgagcagg
    29641 atgtgattct ggtcgatcac gaagaaagaa ttcgtcagct ccgcattgaa gtagatgacc
    29701 atgaaatccg tattgctgcg aatactgcgg caattgcaga gctggatgtc agactaacca
    29761 cggctgaagg agaaatagtc actttacagg ctgacgtcag cgctcttgat ggccgggtta
    29821 cgactgctga gagcaacatt tctgcattac aggacgacta tgtatcgaaa acagccactg
    29881 caacacaatc gctggcatct cccctcaatg taacaacatc ctattcggtc ggcggcacca
    29941 aggttattgg tgctcgtcag acagggtgga cagcagccac aggtacgcca cttcttggct
    30001 cattcaacgc taaccagtca tacactgtcg gcactacgta cacacaatcc gaagtcgcat
    30061 ctctcgctac aggtttgcag caggcgcggc agcgtattct ggcgcttgaa acggcactta
    30121 gattacatgg gctgattgac tgatgattac attcaaacca acgcgaaaca ttgacctgat
    30181 cgaagcagtc ggaaatcacc ctgacattat cgccgggagc aacaacggtg atggatacga
    30241 ctacaaacct gattgccgtt actttgaggt gaacgtgcac gggcagttcg gcggaattgt
    30301 ttactatcag gagattcagc ctttgacctt tgattgccac gccatgtacc tgccagagat
    30361 tcgcggattc agcaaggaaa tcgggctggc gttctggcga tacattctga ctaacaccac
    30421 cgttcagtgc gtcacatcgt tcgctgcacg caaattccgc cacggtcaga tgtactgcgc
    30481 aatgattggc cttaagcgtg taggaaccat caagaaatac ttcaaaggcg tggatgacgt
    30541 gacgttttac agcgccacac gcgaagaact aatcgacttc ctgaatcacg ggagatagcc
    30601 atgttatatg catttaagct gggcagaaaa ctgcgcggcg aggaacctta ttgccctgaa
    30661 aaaggcggga aaggtggcag ttctgataaa agcgcaaagt atgccgcaga agctcagaag
    30721 tatgcagcag acaagcaaga tcagcagttc aacaccatca tgaacaacct gaagccgttt
    30781 actcctctgg ctgagaagta tgtcggcagc ctcgagaact tatcgtctct ggaggggcaa
    30841 ggtcaggcac ttaaccagta ttacaattct cagcagtata aagaccttgc aggtcaggct
    30901 cgctatcaga gtctggcggc agcggaagca acaggtggat tgggttccac cgcaaccagt
    30961 aatcagttag caacaatcgc accaacgctt ggtcagcaat ggctatctgg tcagatgaac
    31021 aactaccaga atctggcaaa tgttggtctt ggcgcactgc aaggtcaggc aaacgccggg
    31081 cagacatatg ccaacaacat gagtcagatt tcgcagcaaa gtgcggctct tgcagcggca
    31141 aatgccaaca gaccatcagc aatgcaatct gctattggcg gaggtgcgtc tggtgctatt
    31201 gctggggctg gacttgcgaa attaattggt tcatccactc cgtggggggc tgcgatcggc
    31261 ggcggtcttg gtctgcttgg ctcgttgttt taaggggtaa tcatggctac gtggcaacag
    31321 ggtattaatt ctggtggttt tctggctggc atcggtatgc aaaatgagaa tgcgccaaag
    31381 gcaaacgaca ttaacgcaac gcttggtctg atccgcgaaa acaatgaact ggctcgctca
    31441 ggtgcaaata acgttggcct gaccgcgtta cgtggtctgg ctggagttgc tgatatttac
    31501 aatcaggaac agcaacagaa agctattagt gcgttcaata aggttcacgc tgatgcatgg
    31561 gcttctggtg atccatcggg actatttaag tttgcccagg aaaatccagc gtttgttgca
    31621 caggcacaac aggcgttttc cggtcttaat gatcagcaac gcaacgatat gggcgattta
    31681 gccatgaggg ctaacgtcgc tctttctcag ggaccggaag cctacagtaa attcattact
    31741 gacaacaagg acaggttaaa tcgcgttggt gctaatgctg actggatgat tcagacaggt
    31801 atccagaatc cagagcagct atcacacatg ctgactacta tgtctctcgg tgcgcttgga
    31861 ccagaaaagg cgtttgctgt tcaggataag atggctggtc gtgaaattga ccgaggcagg
    31921 ctggcagaga caatccgcag caatcaggct ggtgaagcac ttcaggcgag agggcaaaac
    31981 ctttcctatc agtcagcaat gactgggcac aatatcgcag cacaacgctt ggctctggat
    32041 cagcaagagt tcgggtttaa gatgcagcaa gcgcaggaaa aggctcagca gttgattagc
    32101 gaagcaccta agctgtcagt aaacatggaa aaaggcatcg agacggctgt aaacaatgct
    32161 acagcatcat caaactcagc caattctatg agtgcgcttg ctcaacagtt cagagcagaa
    32221 aaaccaacga caggtttgtt cggtaacgca cagaacatgt tcgcaaaact taccggaagc
    32281 gatacaacat tgcgtgattt gcgcattcgc caaaatgccc ttgttaacag tcaggttctt
    32341 aaattcctac ctcccggccc agcaacggat aaagacgttg agatcgttcg acagggtgcg
    32401 ccaactgaca tggataaccc tgagacggtc gcaagatggc ttgatgcaat ggcaaacctt
    32461 gagcgacgaa acgcgcagtt taatgagttt aaagccgagt ggatgagcgc gaatggcaac
    32521 cctggacaat cgcgtaatgg cggtcagata ttggggttgg atgttaaaaa aggtgaatca
    32581 ttggggagtg ccgttaagcg gtatatgtca ttgaatactg acgcagcgcc agcacaagat
    32641 tcgacacctt caggagaacc acgggatcag gttggatcat atacctcaaa atcaggcatt
    32701 caatttacgg tggaatgatg aaagtaactg caaacggtaa gacatttacc ttccctgatg
    32761 gtacgagcac ggaagatatt ggagccgcca ttgatgagta ttttgctggt caggctgttc
    32821 agcaacaaac agttaatcag gccaataatg caccaacacg ggaagaacca tcattgatgc
    32881 aacaagctgg cgattggctc actggtggtc aaagtgcagg gcaaattgca gaacaggctg
    32941 gtcgtggtct ggtaaacata ccatttgacg tattgcaggg tggcgcaagt ctgattaatg
    33001 caatcagtca ggggcttggt gggccaaaag ttttggatga tgtttatcgt ccagtagata
    33061 aaccgacaga cccatacgca caagccggtg aaacaattgg tgggtattta gttccaggag
    33121 ttggaacggc aggaagcatg gctattggat cgctggcaga ggccgcaaat cagaaaggcg
    33181 atttcgcaca aaatgcagct aaaaatgccg gagttaacct tgccgctcag ggtgttcttt
    33241 ccgcagcagc aaagggaata gggcgtggaa taacggctat aaaaggtgat attgcgccag
    33301 aagtggcgaa gaaaattgcc acatcagaat cgatgggcgt gacaccaatg acatctgatg
    33361 ttatcccgcc gaaaaatgct ttcactcgcg gccttactca ggatgccgag ggggctttgc
    33421 tcgggacggg ctcaaagcga gcagagcaat atgcaacgcg tagtaagctg gtaagcaatt
    33481 attttgaccg ttttggtgag tacaaccctg atgatgtggt gaaatctctg accaccacgt
    33541 taagggggcg gaaggatgct gctggcgctg ttatcaatga cgtcaccaat aaaatgggta
    33601 atgccgcagt tgatactaca aataccatga atgctctgaa tacagcgatc gcaagacagg
    33661 aacggcttgg gacttcagcc aatcaaagcc tgcttacatc cttgcgtaac ctgcgtgaag
    33721 aattagcaaa ccctgcaact gatttggatg ttacgtttga tctcttgcgt cagcacagaa
    33781 cagcatttag atctaatgtt cagggagatg ctatggtctt ccccaaccag gcaaaagcag
    33841 ctaccaatat ggtagagaat gcaatgtcaa aagaccttcg taacgcagtt gcaaaaaacc
    33901 tcggtgcgtc agacgcagca aaatacctta aagcaaattc cgattatgca aacgtttata
    33961 ataaggtgct taataaaaac attgctaaca agctcaacaa ggcaagcagt gaagccagtc
    34021 ctgaacttat aaatactgtt gtattaagca gaaaaccatc tgacgtgaaa cgaatctgga
    34081 gcgcattgga tgataaaggg aaagatgcta tgcgtgcagc ttacgtcagc aaaatagcgg
    34141 aaaaggccgg tgactctcca gccaagttca tcactgaagt taataagctg aaatctcagt
    34201 caggcggtga aatttacaac actatttttt ctggaaagca catgaaagag cttgattctc
    34261 ttcatgaagt tctacagcaa acagcaaggt cagacaccgc aaatgtagta actcagacgg
    34321 ggcaatcgca agccaaccgg ataaggacga ttggcgcaac tgcgactctt ggcgtatcaa
    34381 tggggcttga ggctggcttt ggtgcaatga tgcgtttgta tgagtctaaa gcagcaagaa
    34441 acatgcttct ccgccttgca aacgtcaagc ctggaactcc ggcatatgag cgagcgttaa
    34501 atcaggctgc taacgccgtt cgccctctcc taactaacga agctaccagg cagtagaaat
    34561 gaacgccaag gaaggctatt taattctctt ttcaatggct gcaattattg cttttcctga
    34621 tgtttcagga gattttgtag ccatataaga cgaaaaaatc atgtcggtca ttctttcata
    34681 acttactatt tcccacttag ccaatgcatg ggacagtttg tagttgtcat cagttagtgc
    34741 ccttatggaa ttttttaagt gtttattctc ttctgttaat cgcgcaattt ttgtatcaat
    34801 ttcatgtgag cgatctaatt ccttaacctg tttcttgagg gcagctaacc ctgcatatag
    34861 tacgcaacag gatatcccaa gagcgagtac gactatttct aacacaccaa cctccttagt
    34921 ttttcgcagg ataccatgaa aaaagtaaac atcttttgcc tacttcacat ttgaatggtt
    34981 tgtcattagg atgtttccgg ttttttcaaa tatggaaatt gatatgaaga ggattatcgg
    35041 cgttgttgct ggcgctatat tgttatctgg gtgcgcaact attgttggtg acgaaacaca
    35101 gctcgtgcaa gtgaacagca atccttccgg tgcgagcttt aaagtaaaag acgaatcagg
    35161 cgtgattgtt gcgcaaggca agaccccgca aggagtaact cttgccaagt cagatggtag
    35221 ttattttggc aaaaagagct accagatcac tatggaaaag gatgggtacg aaccagttac
    35281 cctgccaatc aaagccaatg ctaatggttg gtatattggt ggaaaccttg tgtttggtgg
    35341 gttaattggt tggcttgctg tagatccttt taatggtggg atgtatacct tgaagccaaa
    35401 agaggcaaac gcatctctta taccatcaac aaagcaagac caataaatag gacccacctt
    35461 caggtgggtt ttttgtacaa atccttcagc gtatcaaaca ccatcttctt aacaagctct
    35521 gactgctcat cagcgagtcg ttctgcatcg tcgcgatatc cagtcacagg cgatggtttt
    35581 gatagagcat cttggacgat ttgtaacaac tcggagttca ttgatctccc attcgcctcc
    35641 gccctgaatt ttaatttctc ccttacttcc ataggcatac ggaagttaaa gtgcggatca
    35701 tctctagcca tgccatcact ccaagttagt gtattgacat gatagaagca ctctactata
    35761 ttctcaatag gtccacggtg gacctatatt gtgaggtgaa tatgaaagga atgagcaaga
    35821 tgccgcagtt caatttgcgg tggcctaaag aagtattgga tttggtacgc aaggtggcgg
    35881 aagagaatgg tcggtctgtt aactctgaga tttatcagag agtaatggaa agctttaaga
    35941 aggaagggcg cattggcgcg taaagttgaa gccccaactg cgggaacagt cagggcttcg
    36001 gtatcaacaa atcggattag gaaatattga catgaaaagt atagcaaagg cacaaaacga
    36061 tttcaccatc ttcaaattcg gcgacagtga aatccgcgtc atcaacaaat gcggtgagcc
    36121 gtggtttgta gcaaaagatg tttgtgatgc tttaaccctg actaactcac gcaaggcgct
    36181 tactgcactt gatgacgatg aaaagggagt aactttaagt tacacccttg gtggtgagca
    36241 gaatctaagc attgttagcg aatcaggtat gtatacattg gttctgcgct gccgcgatgc
    36301 tgtcaataaa ggttcggtcc cgcacaaatt ccgcaagtgg gtaacagcag aagttctacc
    36361 ttcaattcgc aaacatggcg agtatgtgaa aggcaagaaa accactgttg aggaaagaac
    36421 gccgctacgc gatgcagtaa acatgctggt aggaaagaaa ggacttcgct atgacgatgc
    36481 atacaatatg gttcatcagc gttttggtat tgacagcatt gatgaacttt caattgaaca
    36541 aatcccgctg gccgtagagt acatccacag ggtagtgctt gaaggcgagt ttatcggcaa
    36601 acaagagaag aaagcaaacg agctttctgc aaaagaagca aacagccttg tatggctatg
    36661 ggattatgct aaccgctcac aggcattatt ccgcgaactg tatccggcat taaaacaaat
    36721 tcaatcgaac tattccggca gatgctacga ctacggtcat gaattctcgt atgttatcgg
    36781 aatggcgaga gacgttttaa taaaccacac acgagatgtt gatattaatg agccagatgg
    36841 accaacgaat ctttccgcat ggatgagact caagaataaa gaattacctc cttcagtaca
    36901 taactactga cagataacca acgcaacgac ccagcttcgg ctgggttttt ttatgcccaa
    36961 aattcaccgt agccatgctg cggcgattcc ttgtatctgg agcaaattaa atgacagaca
    37021 ttacagccaa tgtgatcgta tcgatgcctt cgcaactttt cactatggcg cgttctttta
    37081 aagcggttgc caatggcaaa atttatatcg gtaaaattga cacggacccc gtaaatccag
    37141 aaaaccagat tcaggtttat atggagaatg aagatggctc tcatgttcct gtttcgcaac
    37201 caatcatcat taacgctgct ggatatccgg tatataacgg acagattgcc aaattcgtta
    37261 ccgagcaagg ccattctatg gctgtttatg atgcatatgg tgctcagcag ttttatttcc
    37321 ctaatgtgtt gaagtatgag ccggatcaat taaggttgga gcttgaatca tctggtggag
    37381 caggattgat aggtggtgtt tcgaagccta ttactttttt tggtgctatt ggtgatggat
    37441 taacaaacaa tacgcaggca tttaacgatg cagagatatc agaattcaat gacatataca
    37501 ttcctgatgg tgtttataat gtaaataata ttaaattaaa taagagatat tgggggcctg
    37561 gcatagtagt gattaattac cagactgaat attctggctc tggtgttaac gacatgaccg
    37621 tgtcaggaat atacaatgga cagcaagatc taaatgcaat tgtaagaata aaagaagctt
    37681 caaatcctga taagcttgaa ttcagtacag ataatggagt tacatggcgt gacacgatag
    37741 acgtgtacaa cccaatcgat gacagtgtat ctccgcaacc aatcgtaata cttgcaggtg
    37801 gggtggattt tttcgtaagt ggattgaaga taaacttttc ttcaaccact ggacatacag
    37861 taaatgactc atggtcattt ttcattcctt ccaatccatc agtaataaac acaggaagtg
    37921 gcagcatcgt taaatcagga agcgttattt ttaatgttag cggaaacaat gatactaata
    37981 cttccgctgg aaaagactca ttaggcggtg gaaacgtagg agctaacaat accgcatatg
    38041 ggtggaagag tttgcactca aacactacag gatacgcaaa taccgcgtct gggattcagt
    38101 ctatggaaaa caacaagacc ggtaaaaaca attgtgcata cggtgctgat tctctaagag
    38161 ctaacgtatc tggttctgat aacgtagcgg ttggggtttt ttcgttaggt gcaaatacaa
    38221 caggctacgg taatactggc ctcggtaatg acgccaatag gtacaatgag acaggattcg
    38281 gaaataccgc agcgggtgtg caggcactgt accacaacaa ggccggtaat gagaacacag
    38341 ctttcggtga atacgcatta cgcggaggaa attccagcct acctacggga acatccattc
    38401 agtattccgt cgctgtagga gctaaagccg gtttcaacgc gttaggtaat aataatacag
    38461 ccataggtta cgaggcacta tattctgcat caggatctga taatgttggt attggattcg
    38521 atgcagggtt taaagtaacg gctggtgctt tcaatgtatt tttaggttct aattctggta
    38581 acgtgtcaga gcaggctaca tatgtgacaa actgcgtcct tctcggagat aatacaaaat
    38641 caaccggaag taacgcagta gctatcggta gtggggcaat tgccgctcag aatactgtag
    38701 ctataggtaa tagtgagcat gaattattct tgcattatgg gaaaattaca ccacaaattg
    38761 ataatgcttt tgatattggt gggccgtatg cgagatataa aacaatatat gcaactacag
    38821 gaatgataaa tacatcaaat gaaatgttga aaacaaaact aacagacatt gatgttgctg
    38881 aaaaaaaagc tgcactagaa ataaaagata atatt
//
LOCUS       DAPGRN010000035        38767 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPGRN010000035 DAPGRN010000000
VERSION     DAPGRN010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.035"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..938)
                     /gene="waaO"
                     /locus_tag="RHW33_001961"
                     /gene_synonym="rfaI"
     CDS             complement(<1..938)
                     /gene="waaO"
                     /locus_tag="RHW33_001961"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HDV1645463.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKTLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCA"
     gene            complement(954..1751)
                     /gene="rfaP"
                     /locus_tag="RHW33_001962"
     CDS             complement(954..1751)
                     /gene="rfaP"
                     /locus_tag="RHW33_001962"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV1645464.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQQDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(1744..2868)
                     /locus_tag="RHW33_001963"
     CDS             complement(1744..2868)
                     /locus_tag="RHW33_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV1645465.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVEANCGEAIVEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2865..3923)
                     /gene="rfaQ"
                     /locus_tag="RHW33_001964"
     CDS             complement(2865..3923)
                     /gene="rfaQ"
                     /locus_tag="RHW33_001964"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV1645466.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            4336..5613
                     /gene="waaA"
                     /locus_tag="RHW33_001965"
     CDS             4336..5613
                     /gene="waaA"
                     /locus_tag="RHW33_001965"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV1645467.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            5621..6100
                     /gene="coaD"
                     /locus_tag="RHW33_001966"
     CDS             5621..6100
                     /gene="coaD"
                     /locus_tag="RHW33_001966"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV1645468.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(6139..6948)
                     /gene="mutM"
                     /locus_tag="RHW33_001967"
     CDS             complement(6139..6948)
                     /gene="mutM"
                     /locus_tag="RHW33_001967"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV1645469.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(7046..7213)
                     /gene="rpmG"
                     /locus_tag="RHW33_001968"
     CDS             complement(7046..7213)
                     /gene="rpmG"
                     /locus_tag="RHW33_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV1645470.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(7234..7470)
                     /gene="rpmB"
                     /locus_tag="RHW33_001969"
     CDS             complement(7234..7470)
                     /gene="rpmB"
                     /locus_tag="RHW33_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV1645471.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(7687..8355)
                     /gene="radC"
                     /locus_tag="RHW33_001970"
     CDS             complement(7687..8355)
                     /gene="radC"
                     /locus_tag="RHW33_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV1645472.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            8527..9747
                     /gene="coaBC"
                     /locus_tag="RHW33_001971"
     CDS             8527..9747
                     /gene="coaBC"
                     /locus_tag="RHW33_001971"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV1645473.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            9725..10183
                     /gene="dut"
                     /locus_tag="RHW33_001972"
     CDS             9725..10183
                     /gene="dut"
                     /locus_tag="RHW33_001972"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV1645474.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            10290..10886
                     /gene="slmA"
                     /locus_tag="RHW33_001973"
     CDS             10290..10886
                     /gene="slmA"
                     /locus_tag="RHW33_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV1645475.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10923..11564)
                     /gene="pyrE"
                     /locus_tag="RHW33_001974"
     CDS             complement(10923..11564)
                     /gene="pyrE"
                     /locus_tag="RHW33_001974"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV1645476.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(11630..12346)
                     /gene="rph"
                     /locus_tag="RHW33_001975"
     CDS             complement(11630..12346)
                     /gene="rph"
                     /locus_tag="RHW33_001975"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV1645477.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            12473..13336
                     /locus_tag="RHW33_001976"
     CDS             12473..13336
                     /locus_tag="RHW33_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV1645478.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            13556..14380
                     /gene="dinD"
                     /locus_tag="RHW33_001977"
     CDS             13556..14380
                     /gene="dinD"
                     /locus_tag="RHW33_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV1645479.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            14671..15288
                     /locus_tag="RHW33_001978"
     CDS             14671..15288
                     /locus_tag="RHW33_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV1645480.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(15285..16967)
                     /gene="ligB"
                     /locus_tag="RHW33_001979"
     CDS             complement(15285..16967)
                     /gene="ligB"
                     /locus_tag="RHW33_001979"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV1645481.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKIALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTTGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDEQS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            17225..17848
                     /gene="gmk"
                     /locus_tag="RHW33_001980"
     CDS             17225..17848
                     /gene="gmk"
                     /locus_tag="RHW33_001980"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV1645482.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17903..18178
                     /gene="rpoZ"
                     /locus_tag="RHW33_001981"
     CDS             17903..18178
                     /gene="rpoZ"
                     /locus_tag="RHW33_001981"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV1645483.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            18197..20305
                     /gene="spoT"
                     /locus_tag="RHW33_001982"
     CDS             18197..20305
                     /gene="spoT"
                     /locus_tag="RHW33_001982"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV1645484.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            20312..21001
                     /gene="trmH"
                     /locus_tag="RHW33_001983"
     CDS             20312..21001
                     /gene="trmH"
                     /locus_tag="RHW33_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV1645485.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITEEALALADRDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            21007..23088
                     /gene="recG"
                     /locus_tag="RHW33_001984"
     CDS             21007..23088
                     /gene="recG"
                     /locus_tag="RHW33_001984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV1645486.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARMAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGKRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(23264..24469)
                     /gene="gltS"
                     /locus_tag="RHW33_001985"
     CDS             complement(23264..24469)
                     /gene="gltS"
                     /locus_tag="RHW33_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV1645487.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            24748..26139
                     /gene="xanP"
                     /locus_tag="RHW33_001986"
     CDS             24748..26139
                     /gene="xanP"
                     /locus_tag="RHW33_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV1645488.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            26260..27969
                     /locus_tag="RHW33_001987"
     CDS             26260..27969
                     /locus_tag="RHW33_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV1645489.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYSLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTGQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDIRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PAQ"
     gene            complement(28022..30340)
                     /gene="yicI"
                     /locus_tag="RHW33_001988"
     CDS             complement(28022..30340)
                     /gene="yicI"
                     /locus_tag="RHW33_001988"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV1645490.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAV
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACTWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARIGNTITVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNAMTITL"
     gene            complement(30350..31732)
                     /locus_tag="RHW33_001989"
     CDS             complement(30350..31732)
                     /locus_tag="RHW33_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV1645491.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            32025..32119
                     /locus_tag="RHW33_001990"
     tRNA            32025..32119
                     /locus_tag="RHW33_001990"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32059..32061,aa:Sec,seq:tca)
     gene            32198..32359
                     /locus_tag="RHW33_001991"
                     /pseudo
     CDS             32198..32359
                     /locus_tag="RHW33_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            32756..33940
                     /locus_tag="RHW33_001992"
     CDS             32756..33940
                     /locus_tag="RHW33_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV1645492.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPIEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            34051..34974
                     /locus_tag="RHW33_001993"
     CDS             34051..34974
                     /locus_tag="RHW33_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1645493.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMDQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(34978..35796)
                     /gene="nlpA"
                     /locus_tag="RHW33_001994"
     CDS             complement(34978..35796)
                     /gene="nlpA"
                     /locus_tag="RHW33_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV1645494.1"
                     /translation="MKLTTHHLRVGAALLLAGILLAGCDQSSSHAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITENPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            36005..36298
                     /locus_tag="RHW33_001995"
     CDS             36005..36298
                     /locus_tag="RHW33_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645495.1"
                     /translation="MKPTMLLMITVFLTFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDESNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            36600..37097
                     /locus_tag="RHW33_001996"
     CDS             36600..37097
                     /locus_tag="RHW33_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone"
                     /protein_id="HDV1645496.1"
                     /translation="MESANLDLEENKEIASKFERALGMGATLAELHGITPDTLEGVYA
                     YAYNFYEKGRLDEAELFFKFLCIYDFQNYNYLKGYAAVCQLKKDYQKAFDMYHICLML
                     SPDNDFSLVYYMGQCQMGLKNIKMATELFNTVVTYSQNEKVKEMATAYLELLTANTEE
                     EVQTE"
     gene            37345..>38767
                     /locus_tag="RHW33_001997"
     CDS             37345..>38767
                     /locus_tag="RHW33_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /protein_id="HDV1645497.1"
                     /translation="MSAPITGQTITFEQISETLRTQYSDAEKRLQDSSKTQVDPMRLN
                     KNPKSLDNDIRARLENKPMLAPPAMQVSDSDNATTAKTNDARLTMILGNLTGIADQDI
                     TTRLHNNLDSTLLRHEMAHNKFRELSDAYASSLDSAQKADDIMHQANNNYNAVDKKVQ
                     SLEKKVNTLNQELSQLQPGDPQYNKVLTQKNAAEKTLTLSLQKKSLAEKSLNTAIMDA
                     DAAIGQSMEIFDEIQQQEQINNFTTNICLTQENQKNRNATATFILLITSVMEVIGDTN
                     CDSIKNQSEVMKEINHVRENKLNETARKYTTTTKVLKIVNECVTVVTIAMSAILIVVG
                     LLAAVPSGGSSIAGALALIGGIAGAVVLGVDITCQIALGTTATGWILGKVVEGLSAAI
                     KTVDPTLLAITALLDVIGVDQNTIDLVKNIYASAAASIIMATVMIGAAVICSVAIGAV
                     VSALSKTAAEEVTKEITNTIKSTI"
BASE COUNT         9835 a        10010 c         9386 g         9536 t
ORIGIN      
        1 ttggcgcaat aacgagcata gtttgagtta attggccgca taaacggttc atttttccag
       61 ggcgatgctt ctttggcatt gataaaaggt tgcgcacttg gataaccggc ccagtaatgc
      121 catggttttg tcgggccgac ataatgaatt aatacggttt catcattaat tggacaaaca
      181 aatgattttt ttaattcata atttaaacta aattgcgtat tgtatttagc atcaatgaat
      241 ttaactttcc ctgacaggat aagattaagg atatcttgat ccatataggt taaacgggaa
      301 acgacggctt tatcagcaag catcgacatc gctttagcag aaacggactc ctgcgcccac
      361 gctagtgtat taattaacag gacacctgaa ttaaagtaac ccttttcaag ttcattacac
      421 tgcagacttt gagcccgtaa cgaccaccag ttagcatcgc gttcagtaac aacagcagca
      481 acgttattgg caagatccat tgttatcagc gtctttaagt ttccctgaca ggcgatatca
      541 gcatccaggt aaaggatctt atcttgttga tcaataaagt aatctgcaat tacaaaacgg
      601 aaatacatgg caatagacca atttttggtc gtcggtaatg ccttaagccg ttcacaattt
      661 actagatgga tctggataca ggtacgatag cttttcgcca attgggctaa acgctggata
      721 tcggcttcag ggatatcatc aataaaaacg tggaaaacaa aactcacgtc gttgttatgt
      781 aacaaaactg acgtgattga aacaccacaa ccaaaaagaa agtttttatc aatgccataa
      841 gcaacatgga atgatgatgc cactgacgcc gctggccttt catcgaagat gattgtctta
      901 ttgatcatct cttgtggatt aaaatagtgg gcactcatat ttctctccgg aaattacaac
      961 gattttcgaa tcgttctttc cctgattttt gtggcttttg cttctgcttg cgacagcagt
     1021 ccttgttcct gcttgagaat gtctttaagc ggggcggcaa aatacacttt cataaaccgc
     1081 cagatatcct gctgagtcag gccgatattc atcgaagaaa aataaagccc aataagatct
     1141 ttatcgcgcc aacgacgtgg aacgcgcgtg cgaagctgcg cccggtgcag gtcaattacc
     1201 gaaattttta actcctcttc cttaccggaa aaaggcaagt gcagcaggaa atgacagata
     1261 taacagtcac ggtggttaat gcccgcagca tgcatatcgc gcaccatcgt cgcgacacgc
     1321 ttaataagca tacgctttac gcgaacatct ggagggttag tcgcccagtc agcacagtaa
     1381 tcttccagac ttatggttgg tgtcagatct tcggtaataa taaatgaagt tctggtcagc
     1441 ggattcatgc ctttttcgcc aaatgccacc ccatacatag tatcaacgcc gacatcccgc
     1501 agtcgatgaa tcgcattcca ttcgcggtct gcgcctaata ctggcatccg caatgagagt
     1561 aaatttttga ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa atagcttttg
     1621 cccgccattt caaagcgcag agtacggcga gtttccagtt cacgaaatac ctcaccctgc
     1681 aaggttttaa cttcctcaaa aggatcttta ccgcgccata acgtggcaaa cggctcttta
     1741 agttcaacca tccagaccac ccgttatgat atccgccgct ttctctggca gactgtataa
     1801 atcttgtgta tcagcataat gtcgcgcatt ttccgcccag gcctggcgca atgaagattg
     1861 cgttaacgct ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct caacaatagc
     1921 ctcgccgcaa ttggcctcga caatataatg cgcatagcca caaacggctg ttgttagtac
     1981 tggtaatcct gcagtaatcg cttccagcag cacaattccc gccgcttcct ggtaggcagg
     2041 atgcagtaat aaatccgccg ccgccattaa ttccgagaca tcgttgcgcc ccgagaagaa
     2101 gtgaacatta ctgcgcacgc cgcgtttttc tgccagcgcc tcaaattttc gcggtttatc
     2161 ctgcccaaca acatatagca atgtgttgtg gcgcagcgaa tccggtaacg aagcaagtgc
     2221 ttcaatggaa cgatcgacac ctttacgcgt gaagtctgaa ccgacctgca acaataaata
     2281 ttgttgttcg gttattccat tcttcttacg gaagatttca cggctattgg ctggctgctg
     2341 gctatattta cgatcaggat aaatccccgg tggcagaata tgaaaacgct ccgcttcagt
     2401 ctgataatgt ttctggaaat cggcgatttg cttatctgtc agcattagca gctgtgtcgg
     2461 cttgccctgt tcgaaggttg ctcgctcaaa ggcggcataa tggcgataac gtgacgtcag
     2521 gcgatagaaa aagccttttt cctgcgctac tttctcggca taacaaacat cagcggcata
     2581 ataaacgtcc agccccggca ttttgttgaa tccaacgact ttatcgacgg gatgttcgcg
     2641 taaatgtttt tgcacccagg caaaatactc cgcattgcgc ccgtgattgg tatgcgattt
     2701 aaccggcact ttgatcagtt caaatacatc agggcattcg ccttcccacg actgggtata
     2761 aacccgaaca tgatgacctc gggcggcgac tgtctgagca atacgcataa aatcgcgctg
     2821 caaaccgcca aagggaaaat atttatataa acaaaaagca acgatcataa cgacgtacct
     2881 gtcgtggagg agggcagtaa tttatcgaca gcagcaatga catctgccgc cggaataacc
     2941 gaaagataca tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg gtaatctccc
     3001 gcccagaatt gaatcatgtt atttgaccag ggacgccaga aaatatggtc tgtcgcacca
     3061 aacagcgata tcagcggcgt attaactgca gcggcaatat gcgccggtgc ggaatcaacg
     3121 ccaataaaca gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt cacctttcca
     3181 gccagcgccg ttactggtgg cgtctggcat ccctgcgcaa tttcattgac gcaggccaga
     3241 tcgtctttat ctgggccgga cgtcagaacg acttcataac cacgagcatg taaggcatca
     3301 atcacagcgg aaaacttggc gttgtcccag catttgaaga tttgccgcgc cgtaggttgg
     3361 ataaccacat aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat acgtttccag
     3421 cttgcaggcg ggtaactcat ggttgtctgc ttcaccaacg aatcaagtcc caatggtgtc
     3481 agcacggata agttactttc caccacattt ccaccctgca acggcaccaa atgggtgaaa
     3541 cttttacgcc aaaaagcaga ctgccgatga tgataatcct gggaaatttt cacacgggca
     3601 tttaataagc gaaccagtat agcaaccatc cattgatcgg taagattgac gataaggtca
     3661 tacttattgg cacgtaatac cttgatgaga tgaaaaaagt tggcaatttt ttctgaggct
     3721 tttgcttttt tattttttat gccgtagagc gcgttaatct ctggattttc agacaggatc
     3781 gggatggtgt cctgataaag cagcacatcg atttttgcgt cagggtaatt ttttttcagc
     3841 gaactaatga cgggagtagt taataacata tccccatgaa aacgcatctt aatgagcaaa
     3901 attcttcgaa atggcttatc cacaagtaac tctttttgtt gagattatcc gattaaggtg
     3961 aagcagaaaa agcacgctac cgccccaggc tcaacagcta cctgaatact gataccgcac
     4021 tgatattgac gcgctagtgt atcacttctt tcgcggcaat ccgacccgaa tgatttttaa
     4081 gcacattgtt gattttcaat aaagaaatca atcaaataaa atcattagca accatattct
     4141 ttcactgttg ctctgcgatt ccttcctgca cgtacagcag aaatcaatga atcgcataaa
     4201 aatacaaaaa aagtatagat gttcagcaag aattggcggg taaattgcta tttaagtcaa
     4261 aaacgggaaa agtaatggta aagccacagc taaatacata gaatccccag cacatccata
     4321 agtcagctat ttactatgct cgaattgctt tacaccgccc ttctctacct tattcagccg
     4381 ctgatctgga tacggctctg ggtgcgcgga cgtaaggctc cggcctatcg aaaacgctgg
     4441 ggtgaacgtt acggttttta ccgccatccg ctaaaaccag gcggcattat gctgcactcc
     4501 gtctccgtcg gtgaaactct ggcggcgatc ccgttggtgc gcgcgctgcg tcatcgttat
     4561 cctgatttac cgattaccgt aacaaccatg acgccaaccg gttcggagcg cgtacaatcg
     4621 gctttcggga aggatgttca gcacgtttat ctgccgtatg atctgcccga tgcactcaac
     4681 cgtttcctga ataaagtcga ccctaaactg gtgttgatta tggaaaccga actatggcct
     4741 aacctgattg cggcgctaca taaacgtaaa attccgctgg tgatcgctaa cgcgcgactc
     4801 tctgcccgct cggccgcagg ttatgccaaa ctgggtaaat tcgtccgtcg cttgctgcgt
     4861 cgtattacgc tgattgccgc ccaaaatgaa gaagatggtg cacgttttgt ggcgctgggc
     4921 gcaaaaaata accaggtaac cgttaccggt agcctgaaat tcgatatttc tgtaacgccg
     4981 cagttggctg ctaaagccgt gacgctgcgc cgccaatggg caccacaccg cccggtatgg
     5041 attgccacca gcactcacga aggcgaagag agtgtggtga tcgccgcaca tcaggcattg
     5101 ttacagcaat tcccgaattt attgctcatc ctggtacccc gtcatccaga acgcttcccg
     5161 gatgcgatta accttgtccg ccaggctgga ctaagctata tcacacgctc ttcaggggaa
     5221 gtcccctcca ccagcacgca ggttgtggtt ggcgatacga tgggcgagtt gatgttactg
     5281 tacggcattg ccgatctcgc ctttgttggc ggttcactgg ttgaacgtgg tgggcataat
     5341 ccgctggaag ctgccgcaca cgctattccg gtattgatgg ggccgcatac ttttaacttt
     5401 aaagacattt gcgcgcggct ggagcaggca agcgggctga ttaccgttac cgatgccacg
     5461 tcgcttgcaa aagaggtttc ctctttactc accgacgccg attaccgtag tttctatggc
     5521 cgtcatgccg ttgaagtact gtatcaaaac cagggcgcgc tacagcgtct gcttcaactg
     5581 ctggaacctt acctgccacc gaaaacgcat tgaggttgtt atgcaaaaac gggcgattta
     5641 tccgggtact ttcgatccca ttaccaatgg tcatatcgat atcgtgacgc gcgccacgca
     5701 gatgttcgat cacgttattc tggcgattgc cgccagcccc agtaaaaaac cgatgtttac
     5761 cctggaagag cgtgttgagc tggcacagca ggcaaccgcg catctgggga acgtggaagt
     5821 ggtcgggttt agtgatttga tggcgaactt cgcccgtaat caacacgcta cggtgctgat
     5881 tcgtggcctg cgtgcggtgg cggattttga atatgaaatg cagctggcgc atatgaatcg
     5941 ccacttaatg ccggaactgg aaagtgtgtt tctgatgccg tcgaaagagt ggtcgtttat
     6001 ctcttcatcg ttggtgaaag aggtggcgcg ccatcagggc gatgtcaccc atttcctgcc
     6061 ggagaatgtc catcaggcgc tgatggcgaa gttagcgtag cgtttatgcc ggatggtatg
     6121 ccatccggcg cgcatgaatt acttctggca ctgccgacaa taaaacgttg cccgctgcgc
     6181 atgtttagtc gccacaatcg gcgtaccgca cacccgacag ggctcgcctt ttcgcccgta
     6241 aacctgcaat tcctgcgcga aatagcccgg tttaccatca ctttgcagaa aatctttcag
     6301 cgttgtacca ccctgctcaa tcgaacgcag caacaccgct ttaatcaccc gagctaacaa
     6361 ttcacactct gccagcgaca gtgatgacgc cagccgatcc ggatggatcc ccgccgcaaa
     6421 cagtgattcg ctggcataga tattccctac ccctaccacc agcttgttat ccatcagcca
     6481 cggtttgatc gccgtttttt tcttcgcgca cttctgatgc aggtactcac cgttgaaatc
     6541 gtcgctaagc ggctccggtc caagatgggc cagcacatta tgcccttcca gctctttggt
     6601 ccacagccag gcaccaaagc ggcgcggatc ggtgtagcgc agcactttac cgttgctcat
     6661 caccaaatcc acatggtcat gcttttcagg gggaagttct tctggaagga tgcgcaggct
     6721 gccagacatc cccaaatgaa tgataatcca gccctcaggc agctccagca gcagatattt
     6781 agcccgccgc tgcacgctaa gcactggttg atcgcttaat cggtagatct cttctgaaac
     6841 cggccagcgc aagcgtccgt tgcgcaccac cgcatgaaga atggttgcac caacgagatg
     6901 cggttctatg ccgcggcggc tggtttcaac ttcgggtaat tcaggcatag catctccagg
     6961 aatgaacaga tgcagtcaat atgggggcaa gcagataaca aaaaaccccg ccggagcgag
     7021 gttttttgtt acatcaaagc ggaaattatt tgattttcgc ttctttgtag atcacgtgct
     7081 ggcgaacaac tggatcgaat tttttcagtt ccagtttttc cggcttagta cgtttgttct
     7141 tcgtagtggt atagaagtga ccagtaccag cagaagaaac cagcttgatt ttctcacgaa
     7201 tacctttagc catgatttat ttcctctaag tacttagtac ttttcgccac gggcacgcag
     7261 ttcagccaga actgtatcga tgcctttttt atcgattaca cgcatacctt tagcagatac
     7321 gcgcagggtg acaaaacgct tctcgctctc aacccagaaa cggtgagagt gcaggttcgg
     7381 caggaaacgg cgtttagtcg cgttcagtgc gtgggaacgg ttgttaccgg tcaccggacg
     7441 cttgccagta acttggcaga ctcgggacat gtctattctc caaaaatcaa attagctcga
     7501 gcttcgtatg gggtattggc gcctcgtcag gctttacagc ccggtcatcg cagttctatg
     7561 tgaactctcg attgccaggc ccaaatgcca aacccgagat tctcaaaggt ggcgtagtat
     7621 acgctgactc agcgatgtgc tcaagtcccg aacagacaaa gatcccgaag gatcgcgcat
     7681 agcgggttaa atccagccgc gttcggcaaa agaaacatac tctccacgcc caatcacgat
     7741 atggtcgagc acgcgtaaat ccatgaactg acaactcttt attatccgtt cagtaatgag
     7801 tttatccgct ttactgggtt cagcacaacc cgaagggtga ttatgtgcaa ggatcagcgc
     7861 cgaggcgttt atttttatcg cttcgcggat aatttcccga ggatggactt caacatggtt
     7921 tagcgtgccg gaaaaaagac ggctatgcgt tataacccgg tgttgtgagt cgagaaagat
     7981 caccataaag atctcccgct cctcacccgt gagctggctt tgtaaaaatt ccctcgtcat
     8041 ctccgggctg agtaaagggc tttcttcacg catccgcacg ttgtagtaac gccgcgccaa
     8101 ttcagcaatc ccctttaact gggcaaattt cgccacgcca attccatgaa caccactaaa
     8161 ttgttcatat tcagaggtta acaagccata aagagagccg aaattctcca gcatctcttt
     8221 tgccagggtt aatacatctt taccgcgcgt tccggtacgc agaaatagcg ccagcagctc
     8281 gacatccgtt aaggcgctaa taccaaactt cagcattttt tcgcgcggca tcaacagctg
     8341 tgcattgttt ttcaccttca cctcctttgt ggtgctcgca tcctgtcata gccgttccgt
     8401 taattcgacg gccagttttc aatcctggaa agcgcctcgc aaagtgattc tcaggtgaag
     8461 gcacgacaac aaaagtattg tgataaaatc gccaacttct ggtgtcaccc aacaggaaaa
     8521 atcatcatga gcctggccgg taaaaaaatc gttctcggcg ttagcggcgg tattgctgcc
     8581 tataaaaccc ctgaactggt gcgtcgtttg cgcgatcgcg gggccgacgt ccgcgtagcc
     8641 atgaccgaag cggcaaaagc ctttatcacc ccacttagct tgcaggcggt ttctgggtat
     8701 cccgtttccg acagtctgct tgacccggca gccgaagccg ctatgggcca tattgagctg
     8761 ggtaaatggg ctgatttagt gattctcgcc cctgccacgg cagatttgat tgcccgtgtt
     8821 gctgccggaa tggcgaatga cctggtatcg acgatttgtc tggctacacc tgcgcctgta
     8881 gccgtgctcc ccgccatgaa ccagcagatg taccgtgccg ctgccacgca gcataattta
     8941 gaggtgcttg cttcccgtgg tttgctcatc tgggggccag acagtggcag tcaggcttgt
     9001 ggtgatatcg gtcctgggcg aatgctcgat ccgttaacca ttgtggatat ggcggtggcg
     9061 catttttcgc ccgtcaacga cctgaaacat ctgaacatta tgattaccgc cggcccgacg
     9121 cgtgaaccgc tcgatccggt gcgttatatc tctaatcaca gctccggcaa gatgggtttt
     9181 gctatcgccg ccgccgctgc ccgtcgtggc gcgaacgtca cgctggtatc aggtccggtt
     9241 tcactaccga cgccaccgtt tgttaaacgt gttgatgtga tgactgcgct ggaaatggaa
     9301 gccgccgtga atgcttctgt acagcagcaa aatattttta tcggctgcgc cgccgtggcg
     9361 gattatcgcg cagctaccgt ggccccagaa aaaatcaaaa agcaggccac gcagggtgat
     9421 gaattaacaa taaaaatggt taaaaacccc gatatcgtcg caggcgttgc cgcactaaaa
     9481 gaccatcgac cctacgtcgt tggatttgcc gccgaaacaa ataatgtgga agaatacgcc
     9541 cggcaaaaac gtatccgtaa aaaccttgat ctgatctgcg cgaacgatgt ttcccagcca
     9601 actcaaggat ttaacagcga caacaacgca ttacaccttt tctggcagga cggagataaa
     9661 gtcttaccgc ttgagcgcaa agagctcctt ggccaattat tactcgacga gatcgtgacc
     9721 cgttatgatg aaaaaaatcg acgttaagat tctggacccg cgcgttggga aggaatttcc
     9781 gctcccgact tatgccacct ctggctctgc cggacttgac ctgcgtgcct gtctcgacga
     9841 cgccgtagaa ctggctccgg gtgacactac gctggttccg accgggctgg cgattcatat
     9901 tgccgatcct tcattggcgg caatgatgct gccgcgctcc ggattgggac ataagcacgg
     9961 tatcgtgctt ggtaacctgg taggattgat cgattccgac tatcagggcc agttgatgat
    10021 ttccgtgtgg aaccgtggtc aggacagctt caccattcaa cctggcgaac gcatcgccca
    10081 gatgattttt gttccggtag tacaggctga atttaatctg gtggaagatt tcgacgccac
    10141 cgaccgcggt gaaggcggct ttggtcactc tggtcgtcag taacacatac gcatccgaat
    10201 aacgtcataa catagccgca aacatttcgt ttgcggtcat agcgtgggtg ccgcctggca
    10261 agtgcttatt ttcaggggta ttttgtaaca tggcagaaaa acaaactgcg aaaaggaacc
    10321 gtcgcgagga aatacttcag tctctggcgc tgatgctgga atccagcgat ggaagccaac
    10381 gtatcacgac ggcaaaactg gccgcctctg tcggcgtttc cgaagcggca ctgtatcgcc
    10441 acttccccag taagacccgc atgttcgata gcctgattga gtttatcgaa gatagcctga
    10501 ttactcgcat caatctgatt ctgaaagatg agaaggacac cacagcgcgc ctgcgtctga
    10561 tcgtgttgtt gctcctcggt tttggtgagc gtaatcctgg cctgacccgc atccttaccg
    10621 ggcatgcgct gatgtttgaa caagaccgcc tgcaagggcg catcaaccaa cttttcgagc
    10681 gtattgaagc gcagctgcgt caggtattgc gtgaaaagag aatgcgtgag ggtgaaggtt
    10741 acaccaccga tgaaaccctg ctggcaagcc agatcctggc cttctgtgaa ggtatgctgt
    10801 cacgttttgt ccgcagcgaa tttaaatatc gcccgacgga tgattttgac gcccgctggc
    10861 cgctaattgc cgcgcagttg cagtaatatg acgccggatg acttttcatc cggcgagttt
    10921 ctttaaacgc caaactcttc gcgataggct ttaaccgccg ccagatgttc cgccatttct
    10981 ggcttctctt ccagataggc aatcagatct ttcagggtaa tgatagagat caccttgcag
    11041 ttgtaatcac gctcaacttc ctgaatcgcc gaaatctcgc cgcgcccccg ttcctgacga
    11101 tcaagcgaaa tcaatacgcc agcaagcgtc gcgccattag cctgaataat ctccatcgac
    11161 tcgcgaatcg ccgttccggc ggtgatcaca tcatctacca gcattacgcg tccttgtaac
    11221 gcgctaccaa ccagattacc gccttcaccg tggtcttttg cctctttgcg gttaaagcag
    11281 tacggcaggt caaggtcatg atgctccgcc agcgccacgg cggtagtggt ggcaatcggg
    11341 atccctttgt aagcagggcc aaacaacaga tcgaactcaa tgccggaatc caccaatgct
    11401 tcagcgtaaa aacggcctaa cagtgccagg tcgcgcccgg tattaaacag cccggcgttg
    11461 aagaaatagg ggcttttgcg cccggatttc agcgtaaact cgccaaactt taacacctgc
    11521 ttgctaagcg caaattcaat aaactggcgc tgatatggtt tcatgccttc gctcctcatc
    11581 ttacttttct acagacaaaa aaaaggcgac tcatcagtcg ccttaaaaat cagtttgcca
    11641 gcgccgcctt ctgcgtcgct acaatggatt cgattccccc tcgggccagc gccaacaagg
    11701 tgagtaactc ttcatgggtg aacggctcgc cttctgccgt cccctgcact tcaatgatgc
    11761 gcccgtcttc ggtcatcact acgttcatgt cggtctctgc ggctgagtct tcaacgtatt
    11821 ccagatcgca aattgcttcg ccgttaacaa taccgacaga aaccgcggct accatccctt
    11881 tcattggatt ggttttcagc ttgccgtttt ccaccagctt ctgtagcgca tctgccagcg
    11941 ccacgcaggc acccgtaatc gacgcggtac gcgtgccacc atcagcttga agcacgtcgc
    12001 agtccaacgt aatggtgaac tcacccagcg ctttcaaatc tactgccgca cgaagagcac
    12061 gggcgatcag acgctggatt tccattgtgc gtccaccctg cttacctttc gccgcttcac
    12121 gagcgttacg ggtgtgggta gaacgcggca gcataccgta ctctgcggtg atccagccct
    12181 ggccctgacc tttcaggaag cgcggcacgc cttcttcaat agaggcggta cacaacactt
    12241 tggtatcgcc aaattcgacc agcaccgagc cttctgcatg ttttgtatag ttacgagtca
    12301 gggtaacggg acgcacctga ttattgctac ggcctgctgg acgcatattg aaatctccgg
    12361 cttgaaacaa atgtggctgc gcattatacg gacttccggc ggttattcct atcctgagaa
    12421 ggcatcgatg gctataatcc ttccacctct ccttttataa acaggaacgt ctatgatccg
    12481 cagtatgacc gcctacgccc ggcgtgaaat caagggtgaa tgggggagcg caacctggga
    12541 aatgcgctcg gtaaaccagc gttatctgga aacttacttt cgtctgccgg agcagttccg
    12601 tagccttgaa cctgtcgttc gcgagcgtat tcgttctcgc ctgacacgcg gtaaagtgga
    12661 atgtaccctg cgctatgagc cagatgttag cgcgcaaggt gagctgatcc tcaacgaaaa
    12721 actggctaaa cagctggtaa ctgccgcgaa ctgggtaaaa atgcagagtg acgaagggga
    12781 aatcaacccg gttgatattc tacgctggcc gggcgtgatg gcagcccagg agcaggatct
    12841 cgacgcgatt gccgctgaaa ttctcgcggc gttggatggt acgctggacg actttatcgt
    12901 cgcgcgcgaa accgaaggtc aggcactgaa agcattgatc gagcagcgtc tggaaggcgt
    12961 caccgccgaa gtggtcaaag tccgcgccca tatgccggaa atcctgcaat ggcagcgtga
    13021 gcgtctggtc gcgaagctgg aagatgctca ggtgcagttg gaaaacaacc gtctggagca
    13081 ggagttggtt ctgctggcac aacgaattga cgttgccgaa gagctggacc gcctcgaagc
    13141 gcatgttaaa gagacctaca acattctgaa gaaaaaagaa gcggttggcc gtcgtctgga
    13201 ctttatgatg caggagttca accgcgagtc gaacactctt gcgtcgaaat ctatcaatgc
    13261 cgaagtgaca aactctgcca tcgagctgaa agtgttgatt gagcaaatgc gtgagcagat
    13321 tcagaacatc gaataactcg taacgccaat tcttactttt ccgccttcac aaatgccgcc
    13381 actcaaacag agcggcattt ttcttccccg caacattcaa atctgttttg cgtgcctgct
    13441 ccagattttg cgatgttttt tgcgcagcac agtgagaacg tgagatactc acaactgtat
    13501 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
    13561 cgaacatcat caaccttttg aagaaatacg gcattatggc actgaagggc aagaattttg
    13621 gtcagcgaga gaattggcac cgttactgga ttatcgggac tggcgcaatt ttcaaaaggt
    13681 actggcccgc gcgactcaag cgtgcgaagc cagcaatcaa gctgcatctg accatttcgt
    13741 tgaaaccacc aaaatggttg tattaggttc aggggctcaa cgagaacttg aagatgttca
    13801 tctctcccgc tatgcctgtt acctggtagt acaaaacggc gaccctgcga aaccggttat
    13861 tgcggcaggg caaacttatt ttgctatcca gacccgacgg caggagctgg ctgacgatga
    13921 agctttcagg caacttcgtg aagacgaaaa gcgtcttttt ctgagaaatg aattgaaaga
    13981 acataacaaa caattagttg aggcagcgca gcaagctggg gtagcgacag ctatcgattt
    14041 cgccatattt cagaatcatg gttaccgggg gctgtatggt ggattagatc agaaagccat
    14101 ccatcagcgg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
    14161 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatggcgt
    14221 gaattcaaaa cagcaagcta acaccacaca ctttgacgtg ggtcgcaagg tgagacaaac
    14281 cattcaggaa cttggcggaa ccatgcctga ggagttgccg accccgcagg tcagcatcaa
    14341 gcaattggaa aacagtgtaa aaattacaga gaagaaatag cagaaactaa aaactctttt
    14401 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    14461 atattgcacc tttcttttct cccctcagtt ttaacctatt ttttcttatg cattttctca
    14521 gacaagaagt cagaagaatg catctctgct acagaaaata gcgatttcac ataactacaa
    14581 gttatcaatt tcccctcacc ttaaaaaatc tcaatcgtga caatgcgcac aaatcgctac
    14641 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    14701 attactgccg aagccatgac gggggctctg gcggccgggc gacgccgcat ggatacattt
    14761 ggcgtaatta ttattgcgac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    14821 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    14881 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    14941 gtgctcgatg cgctcggact ggtagtcttt tctatcatcg gcgcacaggt tgcactggat
    15001 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc
    15061 gttttgcgcg atatgttctg taaacgcatc cctctggtat tccagaaaga gttgtatgcc
    15121 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    15181 gatgtggtga ttatctccac cctggtattc ggcttttttg cccgtttact ggcgctacgt
    15241 cttaagctgg gattaccggt tttttactac agccacgaag gtcactaagg ttcaaaacct
    15301 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    15361 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgttct
    15421 gtgctaaata aaagttgcga ccaggactgt tcatcactgg cattaagcgc cgcccgggtt
    15481 agcggtattc ccattgccat cacccagcga gtaaaaggct gcttacgagc cagattaaac
    15541 tgatgccata gctgcgcact tttacttttc gcgatccccg tcgtgttctg taattgctct
    15601 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    15661 cagccggcct caccaatgcc atccagccca agaacctgtt ttgaccccag ccagactaag
    15721 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgagttaaag
    15781 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    15841 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggtgc aatatcccac
    15901 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    15961 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    16021 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    16081 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    16141 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    16201 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    16261 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac gcccagagaa
    16321 ttcagcgtgt cgctatcgtc ctggcgcatc atcagaccag caacttttgc gcgggcattt
    16381 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    16441 gtactgttgg ctaaaggccc gctaacggtt tgcggcacag cggaaattaa gcgaactttc
    16501 tgcgtccagt cctcgccttt caggccattg ccgcgactga ttgctttgtt cagtttcccg
    16561 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    16621 cgttcgcgca tccacagact taatgcgatt ttatccacca ttttgcgtac acccgtatgg
    16681 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    16741 ccaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    16801 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    16861 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    16921 gattgccagc acaagatact tattaatatc gccatccata ctttcatcat cacctccgct
    16981 gtagatagtc aggcagatat acaacgtgat gaaagcaaag ccgagtggca aaaacggagt
    17041 ctgcgaggac gcttcctgaa aatcgtcttt attgcagtga atgacaggca aatgcggaag
    17101 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    17161 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    17221 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    17281 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    17341 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    17401 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    17461 taattactat ggcacttcgc gtgaggccat tgagcaagtc ctggcgaccg gtgttgatgt
    17521 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    17581 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    17641 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    17701 ttacgccgaa tatgattacc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    17761 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    17821 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    17881 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    17941 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    18001 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    18061 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    18121 agagcaggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    18181 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    18241 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    18301 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    18361 tctggccgag atgaaactcg actatgaaac gctaatggcg gcgctgctgc atgacgtgat
    18421 tgaagataca cccgccacct accaggacat ggaacagctt tttggtaaaa gcgtcgccga
    18481 gctggtagag ggggtgtcga aacttgataa gctcaagttc cgcgataaga aagaggcgca
    18541 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcattctcat
    18601 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    18661 tcgccgcatc gcccgtgaaa ctctcgaaat ttatagcccg ctggcgcacc gtttaggtat
    18721 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaatcgtta
    18781 ccgcgtaatt aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    18841 aatcctctct gaaatcgaag ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg
    18901 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    18961 ctcgatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    19021 cgtgctcggc cagatgcata gcctgtataa gccgcgtccg ggtcgcgtga aagactacat
    19081 cgccattcca aaagcgaacg gctatcagtc gttgcacacc tcgatgattg gcccgcacgg
    19141 cgtgccggtt gaagtccaaa tccgtaccga agatatggat cagatggcgg agatgggtgt
    19201 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc acgactgcac aaatccgcgc
    19261 ccagcgctgg atgcaaagcc tgctggagct gcaacaaagc gccggtagct cgtttgaatt
    19321 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    19381 gcgcattgtc gaactgcctg ccggtgcaac gcctgtcgac ttcgcttatg cagtgcatac
    19441 cgatatcggt catgcctgcg tgggcgcacg cgtcgaccgt cagccttacc cgctgtcgca
    19501 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    19561 cgcgtggctg aactttgtcg ttagctcgaa agcgcgcgcc aaaattcgtc agttgctgaa
    19621 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    19681 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgtat
    19741 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    19801 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagtca
    19861 cggacatctg cccattaaag gtgccgatgg cgtgctgatc acctttgcga aatgctgccg
    19921 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    19981 ccatgaatcc tgccgtaaca tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    20041 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    20101 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    20161 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    20221 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    20281 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    20341 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    20401 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    20461 cgtctggcct ggtagccgca tgcgtaccat ggcctctgcg gcggcgggta gcaacagttg
    20521 ggtgcaggtg aaaacgcacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    20581 catgcaaatt ctggcaaccc atctttctga taacgctgtc gattttcgcg aaattgatta
    20641 cactcgcccg acctgcattt tgatgggtca ggagaaaacg ggcatcactg aggaagcgtt
    20701 ggccctggcg gatcgggaca tcatcattcc gatgattggc atggtgcaat cgctgaatgt
    20761 ttccgttgcc tcagccctca ttctttacga agcccagcgc cagcggcaaa atgcgggcat
    20821 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    20881 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttacc cccacgtcaa
    20941 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    21001 agtgccatga aaggtcgcct gttagatgct gtcccgctca gttccctaac gggcgttggc
    21061 gcagcgctta gtaacaagct ggcgaaaatc aacctgcaga ctgtgcagga tctactctta
    21121 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactaccg
    21181 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    21241 cggatgatga cctgccagat cagcgacggt tccggcattc tcaccatgcg ctttttcaac
    21301 ttcaacgcgg caatgaaaaa tagcctggcg acaggccgcc gcgtgctggc ctatggagaa
    21361 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    21421 ctcagtacgc cagagttgca ggaaacgctc acgccggttt atccaacaac cgaaggcgta
    21481 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    21541 attgaagaac tcctgccgcc ggaactgtca caagggatga tgacgctacc ggaagcgttg
    21601 cgcactttgc accgcccgcc gccgacgctg caacttagcg atctggaaac cgggcagcat
    21661 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc ataacctcag catgttagcc
    21721 ttacgcgccg gagcgcaacg tttccatgcc cagccgctga gcgccaatga cacgctgaaa
    21781 aataaacttc ttgccgcctt accgttcaag ccaacgggcg ctcaggctcg cgtggtggcg
    21841 gagatcgagc gcgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    21901 ggttccggta aaacgctggt cgctgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    21961 caggtggcat tgatggcacc aaccgaatta ctcgccgaac agcacgccaa taacttccgt
    22021 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggtaaa
    22081 gcgcggatgg cacagcagga agccatcgcc agcggtcagg tgcagatgat tgttggtacg
    22141 cacgccatct tccaggaaca ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa
    22201 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    22261 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgact
    22321 gcgtatgccg atctcgacac ctcggtcatt gatgaactgc cgccaggccg cacgccagtg
    22381 actacggtcg ctattcctga tactcgccgt accgacatca ttgaccgcgt gcgccacgcc
    22441 tgcatgactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcggaattg
    22501 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagagttg
    22561 aatgttggcc tggtacacgg gcggatgaaa cctgccgaga aacaggcggt gatggcgtcg
    22621 tttaaacaag gtgaactaca cctgctggtt gccactaccg ttattgaagt cggtgttgat
    22681 gtgcctaacg ccagtctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    22741 caccagctgc gcgggcgtgt aggtcgtggt gcggtggctt ctcactgcgt gctgctctac
    22801 aaaacgccgc tctccaaaac cgcgcaaatt cgcctgcaag tgctgcgcga cagcaacgac
    22861 ggttttgtga ttgcacagaa agacttagag attcgcggac cgggtgaact gttaggtaag
    22921 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    22981 ccggaagttc agcgcctggc acgccatatt cacgaacgtt acccacaaca ggcaaaagcc
    23041 ctgatagaac gctggatgcc ggagacggaa cgttactcga atgcgtaaaa ggcggcggtg
    23101 agaagaccgc catttcaggt taccctacct tcctgccgga tgtgcttcgt cctacaaaaa
    23161 cttataaatt caataaattg tgaatcaatg cgcagccttg ataagcgaag cgtatcaggc
    23221 atttgtcttt gttgcggcgg cacttctacc gccgcttcat cggttaaccc gcaaaaatcg
    23281 gcaacatcaa atacagctta atcaccagcg cgttaacgat atcgataaag aacgcaccga
    23341 ccatcggcac caccagaaac gccatgtgcg acgggccaaa gcgttcagtg atcgcctgca
    23401 tgttggcgat tgctgttggc gttgcaccga ggccaaaacc acagtgaccc gcagccagca
    23461 ctgccgcatc gtagtttttg cccatcatgc gccaggtaac gaagatggca tacaacgcca
    23521 tgaagatggt ctgtaccacc agaatcgcca gcatcggcag cgccagcgaa gccagctccc
    23581 acagtttcag ccccatcaac gccatcgcca ggaacaacga caggcttacg ttacccagca
    23641 cggataccgc acgctcaaag acgcggtaaa agcccatcat tgacagaccg ttgctcagaa
    23701 tcacgccaac aaacaggaca cagacgaagg tcggcagttc aaaagcagtg ccagccaaaa
    23761 gttgcgcaac aattttcccc accgtcaggc agatagcaat cagcgcgata gtttcaatca
    23821 gcaccagcga ggtgatcatg cggcccacat ccggcttttc aaacgccgtc gggacttcct
    23881 ggtcatccgg aataccgttc ggcgtggtgg agtgtttcac cagatagcgc gccaccggac
    23941 cgccaatcaa gccgcccagc accaaaccga acgttgcaca ggccatcgcc acttccgtcg
    24001 cattggtgaa gccataacgt tcaatgaaca atttactcca tgcagctccc gtaccgtgac
    24061 cgccggaaag cgtaatagaa ccggccaaca gccccatcag cggatcaagc cctaacaggc
    24121 tagccatacc aatgccaatg gcattttgca tcaccaacag accaacaacc acaatcagga
    24181 agatgccaac cacacgccca ccggcacgca aactggcaat gttggcgttc aggccaatgg
    24241 tggcgaagaa tgccagcatt aacggatcgc gcagggacat atcaaagttg acttcccagc
    24301 ccatgctttt tttcagtacc agtagcgcca gtgccaccaa caaaccaccc gcaacaggtt
    24361 ccggtatggt gtatttcttc aaaaaggaga cggaatggac caacttacgc ccgagcagca
    24421 acgtcagcgt tgcggcaaca agcgttgcta aagtatcgag atgaaacata gttactcctt
    24481 tgtgtccgca tcactctttc atactcgtca tacttcatcg cgcagtggca acttatgaac
    24541 tatgacgggt atcgagttat gctttcctgg gtcaaattct cagctgttaa tgaacaaatg
    24601 gcatggattt taagcagaaa aataccaaaa gttatataaa aaagtgcatt tatctcattt
    24661 aaatatttag atgaggatgt ggcaaacgtt tgcttttctc tttcgctcag ctaaaatgcc
    24721 cgctttgcta ccacgggatt gttttcgatg tctgtttcca ccctcgagtc agaaaatgcg
    24781 caaccggttg cgcagactca aaacagcgaa ctgatttacc gtcttgaaga tcgtccgccg
    24841 cttcctcaaa ccctgtttgc cgcctgtcag catttgctgg cgatgttcgt tgcggtgatc
    24901 acgccagcgc tattaatctg ccaggcgctg ggtttaccgg cacaagacac gcaacacatt
    24961 attagtatgt cgctgtttgc ctccggtgtg gcatcgatta ttcaaattaa ggcctggggt
    25021 ccggttggct ccgggctgtt gtctattcag ggcaccagct tcaactttgt tgccccgctg
    25081 attatgggcg gtaccgcgct gaaaaccggt ggtgctgatg ttcctaccat gatggcggct
    25141 ttgttcggca cgttgatgct ggcaagttgc accgagatgg tgatctcccg cgttctgcat
    25201 ctggcgcgcc gcattattac gccgctggtt tctggcgttg tggtgatgat tatcggcctg
    25261 tcgctaattc aggtggggct gacctcaatt ggcggcggtt acgcagccat gagcgataac
    25321 accttcggcg caccgaaaaa tctgctgctg gcaggcgtgg tcttagcctt aattatcctg
    25381 cttaaccgtc aacgtaaccc ttacttacgc gtggcctcac tggtgattgc gatggcggcc
    25441 ggatatgcgc tggcgtggtt tatgggcatg ttgccagaaa gcaacgaacc gatgacgcaa
    25501 gaactgatta tggtgccaac gccgctctat tacggtcttg gcattgaatg gagtctgctg
    25561 ctgccgctga tgctggtctt tatgatcact tcgctggaaa ccattggcga tatcacggcg
    25621 acctctgacg tttccgaaca gccagtgtcc ggtccgctgt acatgaaacg cctgaaaggc
    25681 ggcgtgctgg caaacggcct gaactcgttt gtttcggcgg tgtttaacac cttcccgaac
    25741 tcctgcttcg ggcagaacaa cggagtgatc cagttgaccg gtgttgccag ccgctatgtc
    25801 ggttttgtcg tcgcgctgat gttgatcgtg ctgggtctgt tcccggcagt gagcggtttt
    25861 gtacaacaca ttccagaacc ggttctgggc ggcgcaacgc ttgtaatgtt tggcaccatc
    25921 gccgcctccg gtgtgcgtat cgtttctcgt gagccgctga accgtcgggc gattctgatt
    25981 atcgcgctgt cgctggcggt cggcctgggc gtgtctcagc agccgctgat tttgcagttt
    26041 gcccctgaat ggctgaaaaa cctgctctcc tccgggatcg ccgcgggcgg tattactgcc
    26101 atcgtgctga atctgatttt cccaccagaa aaacagtaat tcactggcgg cggtaagata
    26161 attaccgccg cagtctcacc tctttcacca ttcccgcctt gaggattgcg cgtaaatcgt
    26221 gcataactcc cttatgtgca tttcacggga tggaagacca tgaaatttat tgggaagctg
    26281 cttctctaca ttctcatcgc tctgttagtg gtgatcgctg gcctctattc tcttctgcaa
    26341 acccgctggg gagcagaaca tatcagcgca tgggtttccg agaatagcga ctatcatctg
    26401 gccttcgggg cgatggatca ccgtttttcc gcgccatctc atatcgtgct ggagaacgtc
    26461 acatttggtc gtgatggtca gcccgcgacc ctggtggcaa aaagtgtcga cattgcgcta
    26521 agcagtcggc aactgaccga accacgccat gtcgatacca tcctgctgga aaacgggacg
    26581 ctgaatctca ccggccagac cgcgccgcta ccgttcaaag ccgatcgtct gcaactgcgt
    26641 gatatggcgt ttaatagccc gaatagcgaa tggaaactga gcgcgcagcg ggtaaatggc
    26701 ggcgtggttc cgtggtcacc agaagccggt aaagtgctgg gtacgaaggc gcagattcag
    26761 tttagtgccg gatcgctttc gctcaatgat gttcctgcca ccaatgtact gattgaaggc
    26821 agtattgata acgatcgcgt tacgctgact aacctgggtg ccgacatcgc ccgcgggaca
    26881 ttaaccggaa acgcgcagcg taacgccgac ggcagctggc aagtggaaaa tctgcgcatg
    26941 gcggatatcc gtctacaaag cgaaaaatcg ctaaccgact tctttgcgcc attacgctct
    27001 gtcccgtcgt tgcagattgg tcgcctggaa gtgatcgatg ctcgtttgca aggtccggac
    27061 tgggcggtga ccgacctcga tctcagcttg cgcaacatga ccttcagtaa agatgactgg
    27121 cagacacagg aaggcaaact gtcgatgaac gccagcgagt tcatttatgg ttcgctgcat
    27181 ttatttgacc cgattataaa cgcggaattt tccccgcagg gcgtagcgct gcgccagttc
    27241 accagccgct gggaaggggg tatggtcaga acgtcaggga actggctgcg tgacgggaaa
    27301 acgttgatcc ttgatgatgc ggcaattgcc gggctggaat ataccttgcc gaaaaactgg
    27361 caacagttgt ggatggaaac gacacccggt tggttaaaca gcctgcaact gaagagattt
    27421 agcgccagcc gcaatctgat cattgatatc gaccctgact tcccgtggca gctcaccgcg
    27481 ctcgatggtt acggtgccaa tctgacgctg gttaccgatc ataaatgggg cgtctggagt
    27541 ggctcggcga atctgaatgc cgccgccgcg acgtttaatc gtgttgatat tcgtcgccca
    27601 tcgctggcgc taaccgccaa cagcagcacg gtgaatatca gcgaactgag tgcatttact
    27661 gaaaaaggca ttctggaagc caccgccagt gtttcacaaa cgccacaacg tcagacccat
    27721 atcagcctga atggacgcgg tgtgccggtg aatattttgc aacagtgggg atggcctgaa
    27781 ttaccgttga ctggcgacgg caatattcag cttaccgcca gtggcgatat tcaggccaat
    27841 gtcccgctga aacctacggt tagcgggcaa ttgcatgccg tgaacgccgc aaagcagcaa
    27901 gtgactcaaa ccatgaatgc gggcgtagtt tccagtggtg aagttacgtc gacggagccg
    27961 gcgcagtaaa aatcaatctc gccgggtacg actcagacgt acccggcatt ccatcaatag
    28021 attacaacgt aattgtcatc gcattccctt gaggagtcac caccagtccc tgctcacttt
    28081 cagcctgcga accgccttgc agaccattta ctttcacaac attgcgcaaa cacagtgtcc
    28141 agttcttcgc ctcgcccgta ccagtcacag taatcgtgtt gccaatacgt gctgctttta
    28201 aagtaaagat caccgaaccg tcagcagtgg gcacttcaca gacggcttca tgcccgtctt
    28261 gcagattaaa gaggtggaat gctgtacctt cgtgccacgc ataatcggga cgttgatcgt
    28321 tgttgcccag cgccagtagg gtgctatcac gcacataaac gggcagactc aggaagctgt
    28381 gctgctgttt atgccagcga ctaccgtcga gttcatcgtt gtgccacagg tgtgtccaac
    28441 gaccttccgg caggtagaac tgcacatcgc ccgcttcagt gaacacaggc gcaaccatca
    28501 cgttgtcgcc taacatgtat tgacggtcaa ggtaatcaca agccggatcg tccgggaact
    28561 ccatcatcat ggcccgcatc atcggcgtac cccgggcgtt tgcacgtgca gcttcacgat
    28621 acagatacgg catcatgcgg catttcagtt gcgtgaagaa gcgcaccaca tcacaggact
    28681 catcatcgta ggcccacggc acacgataag atttgctgcc atgtaaacga ctatggctgg
    28741 agagcaaacc aaacgcgcac cagcgtttgt aaacgtgcgc cggcgcggta ttctcaaagc
    28801 cgccgatatc gtggctccag aagccaaaac ctgaaaggcc aatggacaaa ccaccgcgca
    28861 ggctttccgc cattgattcg tagttagcgt aacagtcgcc accccagtgt accgggaatt
    28921 tctgcgcacc gacggaggcc gagcgggcaa acaagacggc ttcttcctca ccaacggtgt
    28981 ccttgagcac gttccacacc agttcgttgt agatgtacgc ataatggtta tgcattttct
    29041 gcggatcgga accgtcaaac cactgaacat cggttgggat acgttcgcca aagtcggtct
    29101 taaagcaatc aacgcccatc gcgaccagac ctttcagttt gtcggcgtac catgtgcagg
    29161 catccggatt ggtaaagtca taaatcgcca gacctggctg ccatttatcc cactgccaca
    29221 gcgaaccgtc cgggcgtttg agtaaataac ctttctcttg taactcttta aagacggggg
    29281 atttttgccc aatatagggg ttaatccaga cgcagatttt cagccctttc gctttcaggc
    29341 ggcggatcat cccttccggg tccgggaaag tcagcgggtc ccactcaaaa tcgcaccact
    29401 ggaaggcttt catccagaaa cagtcaaagt ggaaaacatg cagcggcaga ttgcgttccg
    29461 ccataccatc aataaagcta tttaccgtcg cttcgtcgta gttggtggta aatgaagtgg
    29521 ttagccacag accgaaggac cacgcgggcg gcagcgccgg acgaccagta aaacgggtat
    29581 aacgatcaag taccgctttc ggcgtcggac cgtcgataac aaagtattcg agatattcgc
    29641 tctcaacgct gaactgcact ttggagactt tctccgatcc tacttcaaaa gagacacact
    29701 gggggtgatt gaccagcacc ccatagccac ggttagtcat atagaacggg atatttttat
    29761 acgcctgttc agtacttgtg ccgccgtccc ggttccaggt ttctaccgtc tgaccattgc
    29821 gcaccagggc agtaaagcgc tctcccagac cataaactgt ttcgccaacg ccaagatcaa
    29881 gccgctcaaa catataattt cgttgattat tcgtgtcctg cacgtagcca ttatttttca
    29941 cctgactacc ggtaatacgt tcaccgttgc gcagaaaatc gagtgaccag aactcacctt
    30001 tgctgacacg cgcgcttaag ttgccacttt taaacacagc ataacgttct gtgttttcga
    30061 ttgtgacctt cacgtcctgc aaaatattga gcggataatg agggccgtta ttcagcgccc
    30121 cctgaaaatg ctcaatccgc accccgacaa taccttcctg tggggagaaa aagcgcagcg
    30181 taaataaagg cgtgtcaagc tgccaggtac gttcacgtac atcacggggg gcagcataga
    30241 ccaccatttc attaccctgc tgttcaacct cgaacacctg aagcgggtga atcaaattga
    30301 ggccaggttg aatcaaccag tttccatcgc taattttcat tgccgttcct tagttctgca
    30361 cttcttgaga ggtgaattgt tgctggcaac ggcgtttacc ctgagccagc tgttccataa
    30421 cggttttcag attgtgcgtc gtgagtgagt agtagcgttt agcgataatc gcgctcagca
    30481 aataacagat cgccggaaca atcgtgaata gcgcgataat gatgctaatc gtggcgctgt
    30541 tctgcgcttt ttctgccgca tcatatccgc cataagccag catccagccg ataagagcgc
    30601 cgccgaaggc caaccccagt ttgagcacaa acagcgtgcc agcaaaactg atcccggtca
    30661 ggcgtttacc attgcaccac tcgccgtagt cgacggtatc ggacatcatt acccactgga
    30721 taggtgtcac cagttgatgc aacacaccaa tcacgaagat gaagacaaac atagtgatgc
    30781 tggcctgcat gggaacaaag aacatcgcaa ggctaatcac tgccagcagg gcgttcgtcc
    30841 accagaagat agtgacttta catttccagt cggtcagcgg ttttgccagt gcggaaccaa
    30901 tcaggttacc cacgcaataa gtggtgagaa aagcgacaaa cacttccggc gtacccagaa
    30961 tccatgtgac gtaatacatc atcgctccac cgcgtacgca caccgccagg atattgaaaa
    31021 tggttagtaa accgacaatc cgccactggt cgttttgcca gatatcacgt aaatcttccc
    31081 gcatagacgt cgttgtaggt ggtgcttcaa cgcgttcttt agtggtgaag aaacagaatg
    31141 ccagcatcat gaatgccacc acggaaagga ccgcgatacc gccctggaaa ccgagtggtt
    31201 tattatcacc accaattaaa ttaaccaatg gcatcatcag aaccgtagaa agcatgcctc
    31261 ccgcggtcgc cagcacaaaa cgccaggatt gcagcgagat acgctgcgtc gggtcattgg
    31321 tgattacacc acccaatgcg caataaggga tattgacgac ggtataaagt aaggtaagta
    31381 gggtgtaagt aattgctgca taaatcattt ttccgttcat actgagatct ggcgtgctat
    31441 aggccagtac acagacgatc ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt
    31501 taccccagcg agagcgcgtt cgatcggcca acaaccccat gcaaggatcg gaaatcgcat
    31561 ccagtgcacg agcgaccaaa aacatagttc cgacaaatcc ggcaggaatg ccaaaaatat
    31621 cggtataaaa gaacatcata tataacatta cgttatcgaa aataatgtgg ctggcggcgt
    31681 ctcccatgcc ataaccaatt ttctctttaa cggacaacac ttcactcttc atctgctttt
    31741 ccttgatgct ataggggtgc tgagaccgat aacgcatttt gtaaaccatc cctgtgagag
    31801 cgaatattcc gatatctggt tattaattta tgattcttgt tttatgtgat cgtggtagcg
    31861 ttaattccgc tcatatatca ttgtaaaata tgggttttat atgaactata atgctttcgt
    31921 gataatactc tgcgtgtatt aggcggaaaa aactgatctg ggggatgtag aaactcaagg
    31981 aagtagctat aatgcgcccc gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc
    32041 cggtgaggcg gctggacttc aaatccagtt ggggccgcca gcggtcccgg gcaggttcga
    32101 ctcctgtgat cttccgccaa aatgcctctt catacgttct ccaacccctc gtaatactgt
    32161 caatccagat attttatact gctcaatatt caatcggctt caggaatacc tggtcaaagg
    32221 ttttgtgatg gacgatgagc ggctgaagaa tccgcctgtc ggttcttctg tcctcgaacg
    32281 catccgcgat attcgcgcca gcatgcgccg tgtttattta caggtgattt tcccccctgc
    32341 tgactctcag ccaactgtat cgccattaag gtgaagatgt tgttttacac gcttctgcat
    32401 accctttttt atacgactgt tctattcctt taacagctgg caaaaaaact gacctataaa
    32461 cctcaccaat aatcagactg ccatcaagat taatttgttc atttaatata tcaaatcaac
    32521 ttgttttatt tttactatca aaaatagctc attgccttac tataattaat atataaaatc
    32581 ggtgattaat tcttaacatg cttttgctaa taatctcaat tgctggccct ataatattgc
    32641 gctagcattg ttttctggtt gtatcagcga tactcaaaaa ttcttaatac aatactcatt
    32701 cgactggtac ttatttgtaa ctcagttata ttttgtcgcc cggtgattca gaagaatgca
    32761 aaaaacggct accactccat caaaaatact tgatctcact gccgcggcat ttttacttgt
    32821 cgcctttctg acgggtattg cgggcgctct tcagactcct accctaagta tattcctcgc
    32881 agatgaactg aaagcccgtc ctataatggt aggttttttc ttcaccggta gcgctattat
    32941 gggaattctg gtcagtcaat ttctggcaag gcactccgat aaacaaggcg accgtaaatt
    33001 actgattctg ctatgttgct tatttggagt gctggcctgc acgctttttg cgtggaatcg
    33061 caactacttc attctcctct caacgggcgt acttctgagt agttttgctt ccaccgcaaa
    33121 cccgcaaatg ttcgccctcg cccgtgaaca cgccgacaga acaggccgtg agacggtcat
    33181 gttcagtaca tttttacgtg ctcagatctc gcttgcctgg gttatcgggc caccgctcgc
    33241 ttatgaactg gcaatgggat ttagttttaa agtgatgtat ctcaccgctg ccatcgcatt
    33301 tgttgtttgc ggactgatag tctggttgtt tttgccatca atacaaagaa atattcctgt
    33361 cgttacccaa cccatagaaa ttttaccctc cacccacagg aagcgggata cgcggctact
    33421 ttttgtggtc tgttcaatga tgtgggcggc gaataatctc tacatgataa atatgccgct
    33481 atttattatt gatgaactac atctaaccga taaactggct ggagaaatga ttggtatcgc
    33541 tgccggtctg gaaattccga tgatgttaat cgcaggctat tacatgaaac gtattggcaa
    33601 gcgactatta atgctcattg ctatcgtgag tggtatgtgt ttttacgcca gcgtactcat
    33661 ggcgacgact ccggcggttg agctggaatt gcaaattctt aatgccatct tccttggtat
    33721 tctctgtggt atcggcatgc tttattttca ggacctgatg cctgaaaaaa taggctctgc
    33781 gacaacgtta tatgcaaata cttcacgcgt cggctggatt atcgccggct ctgttgacgg
    33841 aattatggtt gaaatctgga gctaccacgc gttgttctgg ctggcgatag ggatgttggg
    33901 tattgcgatg atttgcctgc tgtttattaa agatatttag ctcaccagtc tggactaaga
    33961 aaaatcggcg tggcgcaaac agagccatcc tcgccatttt tgctatcatg cctgcataca
    34021 taaacgacaa aacagtatgc agagggaaaa atgggttcca ccagaaaggg gatgctgaac
    34081 gttctgattg ccgccgtgtt gtggggaagt tcaggggtct gcgcgcaata catcatggat
    34141 caaagccaga tgtcgtcgca gtttttgact atgacgcgtt tgatattcgc cggtttgatt
    34201 ctgctgacgc tgtcatttgt tcatggcgat aaaatctttt ctattattaa caatcacaaa
    34261 gatgccatta gtctgctcat tttttccgtg gttggcgcgc taaccgtaca gctcaccttt
    34321 ttactgacca ttgaaaaatc gaatgctgcc acggcaacgg tgctgcaatt cctctcaccg
    34381 acgattatcg tcgcctggtt ctcactggtg cgtaaatccc gcccgggcat tctggttttt
    34441 tgcgctattt tgacatcgct ggtcgggact tttttattgg tgacacacgg taatccgaca
    34501 tcattatcga tctctcctgc cgcgttattc tggggcattg cctcggcatt tgctgctgca
    34561 ttctatacca cctatccctc aacgctgatt gcccgctatg gcacgttacc agtcgtcggc
    34621 tggagtatgc tgattggcgg tctgattctg ttgccttttt atgccagaca aggaacaaac
    34681 tttgtcgtta acggcagttt gattctggcg tttttttatt tggtggtcat tggtacatct
    34741 ctgacattta gcctgtacct gaaaggagca caattaattg gcggtccaaa agccagcatt
    34801 ttaagctgtg cagaaccatt aagtagcgcg ctgctctctt tgctgttgct ggggatcacg
    34861 ttcacattgc cggactggct gggaacgctg ctgattctgt catcggtaat tttgatttca
    34921 atggattccc gtcgccgcgc cagaaaaata aatcgtccag cgcggcatga gtgagaatta
    34981 ccagccaggc accgcgccac cgttaaagat ggtttctgcc gcttttgcga cttcgggtga
    35041 ttgataagat tgcagaaatt ccttcacgtt ttctgcattc ttattatctt cccgtgccac
    35101 caaaatattc acatacggcg aatttttatc ttcaataaat acgctgtcgt gcaccggaga
    35161 aagcccggtc tgctgaatgt aagtggtgct gataatcgcc acatcaactt tgggatcatc
    35221 cagtacgcgc gggagctgcg ccccctcgag ttccataatc tgcaaatgac gaggattctc
    35281 ggttatatcc agcgcggtag gtagtaaccc ttttccctct ttcagagtaa ttagcttctc
    35341 tttttgcaac agtaaaagcg cgcgaccaag gttggtcgga tcgttgggga tcgccactgt
    35401 cgcgccttct tttatttgcg ccacggtttt tatttttttg gaataacccg ccatggggaa
    35461 gacaaacgta tttcccaccg ctaccagttt ataaccatgc gcctgattat cttgttcaag
    35521 aaagggtcga tgctggaaga cgttcgcatc aagctcgcca tgatttgtcg catcgttcgg
    35581 tagcaacgaa ccgctaaaac ctaccagttc aacatcgagg ccatatttct cttttgccac
    35641 ctttttagcg acttccgcga catcttgttc tgcgccattt ataacgccaa ctttaatgtg
    35701 ttttgcatgg ctgctgctct ggtcgcaacc tgccagcaga attcctgcca gcaataatgc
    35761 ggcccccacc cgtagatgat gtgttgtcag tttcattttt tatccttttt ttaatcatta
    35821 actgactata atggcaagat cactacgatt taaaaaacga aaagctatcg aatagaagaa
    35881 aaaggtataa aaaactggca cttcttactg accacgcaaa cacctttcgt acagcatgat
    35941 gagagcggca aaagcaaact acacttatgc ttcattcgat gcacatttgc agaaggttgc
    36001 cactatgaag ccaacgatgc tactcatgat taccgtattc ctgacttttc cagccatttc
    36061 tcaggccgag tcgccattca gttcgctaca gtcggcaaaa gagaaaacca cggtattgca
    36121 agatttacgt aaaatttgca cgccacaggc gtcattatca gacgaagcgt gggaaaaatt
    36181 aatgttgtct gatgagagta acaaacaaca tatccgcgaa gccattgtgg cgatggagcg
    36241 taataatcag aataactact gggaagcgct gggtaaggtg gaatgcccgg atatgtgaat
    36301 ttcgcacacc tccgcgctca ggccagcagc cgctgactta agctcgcaca tcagtaaatc
    36361 acctgctttt tcaaattcca atcaaaattg aaccagttca tcaggattgt ggataaattt
    36421 ccctctacat tgttttcatt tcatcacata gaaaatctta tctcattgaa acaatacttg
    36481 tttttaatgc agaaaacgaa gcccaacgga tgggcataaa aatgagcgaa ttttgatatc
    36541 accaccctct aaaaaatggt gaagtctgaa caaagaatgt tcgaaaggag agagtagaga
    36601 tggaaagcgc aaatctggat ttagaagaaa ataaagaaat tgccagtaag ttcgaacgtg
    36661 cactaggtat gggagcaacg cttgctgagc tgcatggcat tactccggat acccttgaag
    36721 gcgtttatgc ttacgcttat aacttttatg aaaaaggaag acttgacgaa gctgaactct
    36781 tttttaaatt tctctgtatt tatgatttcc aaaattataa ctacttaaaa ggatatgccg
    36841 ccgtttgtca gttgaagaaa gattatcaaa aggcattcga catgtatcat atctgtttaa
    36901 tgctatctcc cgataacgat ttctctttgg tttattatat gggccaatgc cagatgggct
    36961 taaaaaacat taaaatggcc acggagttat ttaatacagt cgtaacctac agtcaaaatg
    37021 aaaaagttaa agagatggct actgcttatc ttgaactttt gacagccaat accgaagagg
    37081 aagttcaaac agagtgagtc ggcaaaatga acataaacgg gaaaaaatct tcttttacgc
    37141 ctgaagagtt aaccaggaaa tatcatgagg aagccccggt tgatttaaaa aataaccatg
    37201 tcgataaaat aaatgagaag tataacaata aacacattac ttactttccc gttgatgcca
    37261 ttttagatgg gaataaacat ttgccatgag ttaaataaaa aagaattaat tacacccggt
    37321 gatagcttaa aaaggaggtg aattatgtcg gcaccgatca cgggacaaac gatcacgttt
    37381 gaacaaatca gcgaaacatt gcgtacacag tatagcgatg cggaaaaaag actgcaggac
    37441 agcagcaaaa cgcaagttga tcccatgcgg cttaataaaa acccgaaatc ccttgataac
    37501 gatattcgcg ctcgtcttga aaataaacct atgcttgctc cgcctgcaat gcaggtttca
    37561 gattcagaca acgccactac agcaaagacg aatgatgccc gcctgacaat gattttgggt
    37621 aatttaacag gcattgccga tcaggatatt acaacacgat tgcataataa ccttgacagc
    37681 accctgcttc gacacgaaat ggctcataat aagtttcgtg aattaagcga cgcctacgcc
    37741 tcgtcattag attctgcgca aaaagctgac gatatcatgc atcaggccaa caataattac
    37801 aatgcggtcg ataaaaaggt gcaatcgctg gagaaaaaag tcaatacatt gaatcaagag
    37861 ctgtcgcaac tgcaacctgg cgatccgcaa tataataaag tactgacgca aaaaaatgcg
    37921 gctgaaaaaa cactcacgct gtcattacaa aaaaaatcgt tagctgagaa atcgttaaat
    37981 acagccatta tggatgctga tgctgcgatc ggccaaagta tggaaatttt tgacgaaatt
    38041 caacagcaag aacagattaa taactttacc accaatattt gcctgaccca ggaaaaccag
    38101 aaaaacagaa acgcgacagc cacatttatc cttttgatta cctcagtaat ggaagttatt
    38161 ggtgatacca attgcgactc tattaaaaat caatctgagg taatgaaaga gattaaccat
    38221 gtcagggaaa ataaacttaa tgaaacagcg cgtaaatata ctaccacgac taaagtttta
    38281 aaaatcgtta atgagtgtgt aactgtagtt accattgcca tgagcgcaat cctaattgtt
    38341 gttggtcttc ttgctgcagt tcctagtggt ggttcaagta ttgcgggtgc actggcgctt
    38401 attggtggaa tagcgggcgc agtggtatta ggtgttgata tcacttgtca aatcgctctg
    38461 ggtaccacgg ctaccggctg gattttaggg aaagttgttg aaggtctttc ggctgctatt
    38521 aaaacagttg atcccactct gctcgcaatc actgcgcttc tggacgttat tggtgtcgat
    38581 caaaatacga ttgatctggt taaaaatatt tacgcaagtg cagccgcttc tattatcatg
    38641 gcgacagtaa tgatcggtgc agctgtaata tgttctgtag caataggggc cgttgtttct
    38701 gcattatcaa aaacagccgc cgaagaagtc acaaaagaaa taacaaacac tatcaaatca
    38761 accattg
//
LOCUS       DAPGRN010000036        38321 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPGRN010000036 DAPGRN010000000
VERSION     DAPGRN010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.036"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..914
                     /locus_tag="RHW33_001998"
     CDS             3..914
                     /locus_tag="RHW33_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645498.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFCNNIHHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKSGYQVLSSKNETVHAMDKFLISFSLKDNGAEYIMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIRQ
                     AIGDVSLGYALLDDIVSKLKRGKFELQGPGGGIKQCDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     DITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(1004..1558)
                     /locus_tag="RHW33_001999"
     CDS             complement(1004..1558)
                     /locus_tag="RHW33_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV1645499.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            1883..2089
                     /locus_tag="RHW33_002000"
     CDS             1883..2089
                     /locus_tag="RHW33_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV1645500.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(2168..3511)
                     /locus_tag="RHW33_002001"
     CDS             complement(2168..3511)
                     /locus_tag="RHW33_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV1645501.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKTTALSPKLPDAKDKEESVA"
     gene            complement(3834..4472)
                     /gene="msrA"
                     /locus_tag="RHW33_002002"
     CDS             complement(3834..4472)
                     /gene="msrA"
                     /locus_tag="RHW33_002002"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV1645502.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            4678..6411
                     /gene="tamA"
                     /locus_tag="RHW33_002003"
     CDS             4678..6411
                     /gene="tamA"
                     /locus_tag="RHW33_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV1645503.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6408..10187
                     /gene="tamB"
                     /locus_tag="RHW33_002004"
     CDS             6408..10187
                     /gene="tamB"
                     /locus_tag="RHW33_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV1645504.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEINSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            10190..10531
                     /locus_tag="RHW33_002005"
     CDS             10190..10531
                     /locus_tag="RHW33_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV1645505.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            10743..10994
                     /gene="chpS"
                     /locus_tag="RHW33_002006"
     CDS             10743..10994
                     /gene="chpS"
                     /locus_tag="RHW33_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDV1645506.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            10988..11338
                     /gene="chpB"
                     /locus_tag="RHW33_002007"
     CDS             10988..11338
                     /gene="chpB"
                     /locus_tag="RHW33_002007"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDV1645507.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLHTRLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(11415..11945)
                     /gene="ppa"
                     /locus_tag="RHW33_002008"
     CDS             complement(11415..11945)
                     /gene="ppa"
                     /locus_tag="RHW33_002008"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV1645508.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAD
                     AAKAEIVASFERAKNK"
     gene            12255..13211
                     /gene="ytfQ"
                     /locus_tag="RHW33_002009"
     CDS             12255..13211
                     /gene="ytfQ"
                     /locus_tag="RHW33_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV1645509.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13351..14853
                     /locus_tag="RHW33_002010"
     CDS             13351..14853
                     /locus_tag="RHW33_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1645510.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETCELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            14867..15889
                     /locus_tag="RHW33_002011"
     CDS             14867..15889
                     /locus_tag="RHW33_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1645511.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            15876..16871
                     /gene="yjfF"
                     /locus_tag="RHW33_002012"
     CDS             15876..16871
                     /gene="yjfF"
                     /locus_tag="RHW33_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV1645512.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(16904..17902)
                     /gene="fbp"
                     /locus_tag="RHW33_002013"
     CDS             complement(16904..17902)
                     /gene="fbp"
                     /locus_tag="RHW33_002013"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV1645513.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            18078..19451
                     /gene="mpl"
                     /locus_tag="RHW33_002014"
     CDS             18078..19451
                     /gene="mpl"
                     /locus_tag="RHW33_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV1645514.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(19607..20158)
                     /locus_tag="RHW33_002015"
     CDS             complement(19607..20158)
                     /locus_tag="RHW33_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV1645515.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            20252..21604
                     /gene="pmbA"
                     /locus_tag="RHW33_002016"
     CDS             20252..21604
                     /gene="pmbA"
                     /locus_tag="RHW33_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV1645516.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDIEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            21788..22174
                     /gene="cybC"
                     /locus_tag="RHW33_002017"
     CDS             21788..22174
                     /gene="cybC"
                     /locus_tag="RHW33_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV1645517.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNSYHKKYR"
     gene            complement(22219..22683)
                     /gene="nrdG"
                     /locus_tag="RHW33_002018"
     CDS             complement(22219..22683)
                     /gene="nrdG"
                     /locus_tag="RHW33_002018"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV1645518.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(22841..24979)
                     /gene="nrdD"
                     /locus_tag="RHW33_002019"
     CDS             complement(22841..24979)
                     /gene="nrdD"
                     /locus_tag="RHW33_002019"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV1645519.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(25373..27028)
                     /gene="treC"
                     /locus_tag="RHW33_002020"
     CDS             complement(25373..27028)
                     /gene="treC"
                     /locus_tag="RHW33_002020"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV1645520.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKKLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(27078..28499)
                     /gene="treB"
                     /locus_tag="RHW33_002021"
     CDS             complement(27078..28499)
                     /gene="treB"
                     /locus_tag="RHW33_002021"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV1645521.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(28618..29565)
                     /gene="treR"
                     /locus_tag="RHW33_002022"
     CDS             complement(28618..29565)
                     /gene="treR"
                     /locus_tag="RHW33_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV1645522.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            29750..29803
                     /gene="mgtL"
                     /locus_tag="RHW33_002023"
     CDS             29750..29803
                     /gene="mgtL"
                     /locus_tag="RHW33_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV1645523.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            29944..32640
                     /gene="mgtA"
                     /locus_tag="RHW33_002024"
     CDS             29944..32640
                     /gene="mgtA"
                     /locus_tag="RHW33_002024"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV1645524.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(32846..33232)
                     /gene="ridA"
                     /locus_tag="RHW33_002025"
     CDS             complement(32846..33232)
                     /gene="ridA"
                     /locus_tag="RHW33_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV1645525.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(33305..33766)
                     /gene="pyrI"
                     /locus_tag="RHW33_002026"
     CDS             complement(33305..33766)
                     /gene="pyrI"
                     /locus_tag="RHW33_002026"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV1645526.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(33779..34714)
                     /gene="pyrB"
                     /locus_tag="RHW33_002027"
     CDS             complement(33779..34714)
                     /gene="pyrB"
                     /locus_tag="RHW33_002027"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV1645527.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(34718..34852)
                     /gene="pyrL"
                     /locus_tag="RHW33_002028"
     CDS             complement(34718..34852)
                     /gene="pyrL"
                     /locus_tag="RHW33_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV1645528.1"
                     /translation="MVQYVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(35133..35528)
                     /locus_tag="RHW33_002029"
     CDS             complement(35133..35528)
                     /locus_tag="RHW33_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV1645529.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGS
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDNIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(35659..36372)
                     /gene="bdcA"
                     /locus_tag="RHW33_002030"
     CDS             complement(35659..36372)
                     /gene="bdcA"
                     /locus_tag="RHW33_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1645530.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            36443..37036
                     /locus_tag="RHW33_002031"
     CDS             36443..37036
                     /locus_tag="RHW33_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV1645531.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVTEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            37181..37633
                     /gene="tabA"
                     /locus_tag="RHW33_002032"
     CDS             37181..37633
                     /gene="tabA"
                     /locus_tag="RHW33_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV1645532.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            37756..>38321
                     /locus_tag="RHW33_002033"
     CDS             37756..>38321
                     /locus_tag="RHW33_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645533.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIVGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKEIARLLNN"
BASE COUNT         9324 a         9453 c        10257 g         9287 t
ORIGIN      
        1 atatgttacc caggatcaga cacaataatt ttattggtgc ggtggagtta tttgtaaagt
       61 cttcgtatac aaaaacacat tcaaacaatt tttgtaacaa catccatcat gcatttaaga
      121 aaaaagattg gatttcgaat tatgatagcc tgttaacttt gagggagttc tttcgttgcg
      181 caactcagat tgataaaagt ggttatcagg tgctttcatc aaaaaatgaa actgttcacg
      241 ccatggataa gtttcttatc agtttcagcc ttaaagataa cggtgccgaa tatataatga
      301 cattgcgagg cagtggattt gaatacgaag aaattcccat aacaataaat gagtataact
      361 cattcatgga tttcaaaaac cgcgagtttc cgttagaaca aaacagaaga ttgtatgcct
      421 gggatatttt gcagaaaaaa cagtctgaca taccgaaaag gattaaaggt tatattcgcc
      481 aggcgattgg tgacgtgtct ttaggttatg ctttattgga tgatatagta agcaaattaa
      541 aacggggtaa atttgaacta caagggccag gaggtggaat taaacagtgc gatggatggt
      601 atatttacga aaaaataatt gatgataatt ttgccatagt catagaatct ttgggctttg
      661 cgcttaaaat ctatggaggg gatgaacgtt ttcgtaatgg ttcctctgtt gttttggagg
      721 atgaagatta ctctcttatt tataattttc tggttaatgc aggttgccaa caagtcgaat
      781 tagccgaaca agtggatgcg atagtaagtg cgaatctggc cgcagatagt gatattacta
      841 aagaaaagat ttgcgagaaa tataaatcga ccattgaggc gtttaaaaaa gagcaactag
      901 cgttaccagt atgagttcgg cgtaagaact cagagactta attcccctat cccatagata
      961 acgatagggg aaaattattt ttgcagaatt tatgttattg ccattatttg agcaactttt
     1021 gcagcaggtc catcacttgc tgcacctctt ccggtgtgag cgccccgtct ttggcccatt
     1081 gcacgcgacc gtctttatcc agcaccacta cagcggaact ctcttcatcc agctgccagg
     1141 caccgcgcgc gacgccattg ctatcgacaa taaactgcga ccagggataa agctttttat
     1201 tactctccag actgctgcgc acaaacatgc cggaacccgg aatcgcgtcg tcggtgttaa
     1261 caatggtggt ggtctggtaa cgatcgtgcg gtaacttcgc tgatttaatc gcttcaatca
     1321 gcgtcgcgtt tttctctttt gcagaggtgc gaccagcaat atgttgcagt actcgcactt
     1381 ttcccactaa ctgcgcgctg ttccaggttt tgtagctaaa ctgatcttta tcaagcacca
     1441 actcgccccg atcggtaatg ccaatcggcg gcactcgctg accggtttcg aactgatgtg
     1501 cggaagccat catcggcaac agcaggcagg tgagtgccag aatcttgcgt agggtcatgg
     1561 tgtttccttc ttatgatatg caggtgatcc gaccacttgg gtctgagttt taatcatatg
     1621 tgctatttat cgaatttccc gcaagtgtga tgccagtttg cggtcaagcg cacaaatcat
     1681 atgaaaaatg aatgcttata ctgaagaccg cgcttcggta aaaagataat tctgaataat
     1741 tgtaaccttt aggtaaaaaa agttatacgc ggtggaaaca ttgcccggat agtctatagt
     1801 cactaggcat taaaatttgc gcctcataat agttgggccg attgtggcac cgcacaggcg
     1861 taatactcag caggagataa caatgaaaat tttccaacgg tacaacccac ttcaggtggc
     1921 gaagtacgta aagatcctgt tccgtggacg gttatacatc aaggacgttg gcgcttttga
     1981 attcgataag ggtaagattc ttatcccaaa agtgaaggat aagctgcatt tgtcagtgat
     2041 gtccgaagtt aaccgtcagg ttatgcgtct gcaaacagag atggcttaac caaagtgcta
     2101 tgcagtaaaa agtgctatgc agtaataaga cggctcctga ttcaggagcc gttgatgttt
     2161 ctgggggtta cgcgacgctt tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg
     2221 cggtggtctt gctgtcgatc cgggtcacca gcagctggtc gatgcggtag ttatcgatat
     2281 ccaccacttc aaatttgtag ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac
     2341 gcagcataaa catcataaag ccgccgatgg tttcgtagtt gcccgactgc gggaactcgt
     2401 caatatccag cacgcgcatg acgtcgtcaa tcggagtgcc gccgtcaatc agccatgagt
     2461 tctcatcacg ggcgacaatc tgctcttcca gtccctgacc gaccagatcg cccatcagcg
     2521 tggtcatcac gtcgttgagg gtgatgatcc ccactaccag cgcatattcg ttcatgatca
     2581 ccgcgaagtc ttcacctgcg gttttaaaac tttccaacgc ctctgaaagg gttaacgtat
     2641 ccggcacaat cagcgtgttg cgaatttgta cgccgctgtt cagtgccagg ctttggttag
     2701 ccagcacgcg gttcagcagg tctttagaat cgacatagcc gatgatgtgg tcaatatctt
     2761 cattacagac gaggaactta gagtgcggat gttccgccac cttatttttc agactttgct
     2821 catcttcgtg gagatcaaac caaatcacgt tttcacgcgg tgtcattgaa gacggaacgg
     2881 tacgggattc cagctcaaag acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc
     2941 ccgccagcgc accggcttcc actaccgcgt agatgtcatc agaagtgatg tcatctttac
     3001 gtaccattgg cagtttgaaa atacggaaga tcatgttcgc caggccgttg aagaaccaca
     3061 ccagcggggt gcaaacgtac aggcagaagc gcatcgggtt gatgatacgc aaagccaccg
     3121 cttctggcgc aatcataccg atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc
     3181 cagtcactaa cgagaaagag agaataaagc tcagttgctc agagagctct gccgacatat
     3241 agcgggagaa caggctgtga aaagctggag aaaatgccgc atcaccgacg ataccgccga
     3301 gaatcgccac tgcgttcaga ccgatttgga ccacggtaaa gaacatgccg ggattttcct
     3361 gcatattcag aacgcgttgg gcatttatat tgccttcatc agccagcagt ttaagtttga
     3421 ttttgcgtga ggcggcaagc gagatctcgg acatcgagaa gaacgcactt acagcgatca
     3481 agcagagtat gactaaaata ctgtttaaca tatcttatcc gacctttcag gccagatcct
     3541 cggaagggga agttgattaa tttgtgtgaa atacacattg aaagccgatt cgaagaaagt
     3601 gaaccggcat tttcagtggc tagtatagcg taaggtactg taaagtcgcc agagggttaa
     3661 aattcagact gctgataaat gcaatactgc ctgatgcgct atgccgatca ggcctacgca
     3721 gtccttgcaa tatattgaat ttggacgatt ttgtaggcct ggtaaggcgt ttacgccgca
     3781 tccggcataa acaaaacgca ctttatcaac aatctaaatt tgtacccgta acgctatgct
     3841 tccggcggca gacagacgcc aattccgcca attccacagt aaccatacgg gtttttatgc
     3901 agatattgct ggtggtcatc ttcggcataa taaaacggtg tggcgttagc gatttccgtg
     3961 gtgatgcgac gatcgtcatc ggcggcaagc atcgccgcct gaaaacgttc cagactggcg
     4021 cgagctgcgg catcctgttc tggggtcagc ggataaatcg ctgaacgata ctgcgtgccg
     4081 tggtcattgc cctgacgcat tccctgggcg ggatcgtgat tctcccaaaa tacctgtagc
     4141 aactgctcat agctgatgac ggaaggatcg taaacaatgc gtaccgcttc ggcatgaccc
     4201 gtatcaccgg agcacacttc ccgataagtc ggatttggcg tatagccgcc ggtatagcct
     4261 gcggcggtgc tgtaaacgcc gggtaactgc cagaacagac gctccacacc ccagaaacaa
     4321 cccatcgcaa aaatggcaat ctccattccg tcaggtacat tggtcattga gtgaccgttg
     4381 accgcatgca gcgtggctac gggcatcggg gtgttacgtc caggcagggc atcggcgggg
     4441 gaaaccagat gctttttatc aaataaactc atggtgtcgc tctcccgaaa atcggtcatt
     4501 ggggttaagg ttgtaacaag aggcgtattt gcacacaata accaccgtga ataggtctaa
     4561 agtaaaacat aagaaatatt tggggtttag tctgcttttt aatccatatt actggatttt
     4621 tgttaagccg tttaacggcg ttccaggggc aggaaaaaag gatattcagg agaaaatgtg
     4681 cgctatatcc gacagttatg ctgtgtaagc ttactctgct taagcggatc tgccgtcgcc
     4741 gcgaacgtcc gtctacaggt cgaggggtta tcgggacagc tggaaaagaa cgttcgtgcg
     4801 cagctttcta cgattgaaag tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc
     4861 gatgatgcca tccgcgaagg tctgaaagcg ctgggttatt accagccgac cattgaattt
     4921 gatctccgtc caccgccaaa gaaagggcgg caggtattga tcgccaaagt cacgccaggc
     4981 gtgccggtgt taattggcgg caccgatgtg gtattgcgcg gcggcgcgcg gaccgataaa
     5041 gactatttga aattgctcga tactcgcccg gctattggca cggtactgaa ccagggcgat
     5101 tatgaaaatt tcaaaaagtc cttaaccagc attgcgttgc gtaaaggtta tttcgatagc
     5161 gaatttacca aagcgcagct gggcattgcg ctcggcctgc ataaagcctt ctgggatatt
     5221 gattataaca gtggcgaacg ttaccgcttt gggcatgtga cctttgaagg atcacaaatc
     5281 cgcgatgaat acctgcaaaa tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa
     5341 gatctggcag aactgaaccg ccgactttct gctaccggct ggtttaactc ggtggtggtg
     5401 gctccacaat ttgataaagc gcgcgaaacg aaagtattac cattgacggg cgtggtttcg
     5461 ccgcgaacag aaaacaccat cgaaaccggg gtcggttact ctacggacgt gggaccgcgc
     5521 gtgaaagcga cgtggaaaaa gccgtggatg aactcttatg gtcacagtct gaccaccagt
     5581 accagtattt ccgcgccgga acagaccctc gacttcagct ataaaatgcc gctgctgaag
     5641 aatccactgg aacaatatta tttggtgcag ggcggtttta agcgcactga cctgaacgat
     5701 accgagtctg actccactac gctggtggct tctcgctact gggatctctc cagcggctgg
     5761 cagcgtgcca ttaacctgcg ctggagtctc gaccacttta ctcagggtga aattaccaac
     5821 accacgatgc tgttttatcc tggggtgatg attagccgca cgcgttctcg tggtggcctg
     5881 atgccaacct ggggcgactc gcaacgctac tctatcgact actccaacac ggcctggggt
     5941 tcagatgtcg atttctccgt tttccaggcg cagaacgtct ggatccgcac actgtacgat
     6001 cgccatcgtt ttgttacacg cggcacgctg ggctggattg aaaccggtga tttcgacaaa
     6061 gtaccgccgg atctgcgttt cttcgccggg ggcgatcgca gtattcgcgg ctacaaatac
     6121 aaatctatcg ccccgaaata cgctaacggt gacctgaaag gggcctcgaa gttgataacc
     6181 ggatcgctgg agtaccagta caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat
     6241 agtggcgaag cggtaagcga tattcgccgc agcgacttta aaaccggtac cggggtcggc
     6301 gtacgctggg aatcgccggt cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat
     6361 aaagacgaac acgggttaca gttttacatc ggtctggggc cagaattatg agtttatgga
     6421 aaaaaatcag cctcggcgtg gttatcgtta tcttactgtt gctgggatcg gtggcgtttc
     6481 tggtgggcac caccagcggc ctgcatctgg tatttaaagc ggcagatcgc tgggtgccag
     6541 gactggatat tggcaaggtc accggcggct ggcgcgatct caccttgtct gacgttcgtt
     6601 atgagcagcc aggcgtggcg gtaaaagcgg gtaatctgca tctggcggtc ggtcttgagt
     6661 gcctgtggaa cagtagcgtt tgtattaatg acctggcgtt gaaagacatt caggtcaaca
     6721 tcgacagtaa aaaaatgcct ccttctgaac aggttgaaga agaggaagat agcggtccgc
     6781 tggatctctc cacgccgtat cccatcaccc tgacacgggt ggcactggac aacgtcaaca
     6841 tcaagattga tgacaccacg gtatcggtga tggacttcac ctccggcctg aactggcagg
     6901 agaaaaccct gaccctgaaa ccgacgtcgc tgaaaggcct gctgattgct ctgccgaaag
     6961 tggcggaagt ggcgcaggaa gaagtggtcg aaccgaaaat cgaaaatccg cagccggatg
     7021 aaaagccgct cggcgaaacg ctgaaagatc tcttttctcg cccggtattg ccggaaatga
     7081 ccgacgtgca tttgccgctt aacctgaaca ttgaagagtt taagggcgag cagctgcgcg
     7141 tgacgggcga cacggacatc accgtgagca ccatgctgct gaaagtgagc agcattgacg
     7201 gcaatactaa actggacgcc ctggatatcg attccagtca agggatcgtc aacgccagcg
     7261 gcacggcgca gctgtcagac aactggccgg tggatatcac cctcaacagc acactgaacg
     7321 tggagccgtt gaaaggtgaa aaggtgaagc tgaaagtggg cggcgcgctg cgcgaacagc
     7381 tggagatagg cgttaacctt tccggtccgg tggatatgga tttacgcgcc cagacgcgac
     7441 tggcggaagc cggattgccg ctcaacgtgg aaataaacag caaacagctt tactggccgt
     7501 tcactggtga gaagcagtat caggcggatg atctgaaact gaaacttacc ggcaaaatga
     7561 ccgattacac gctctctatg cgtacggcag tgaagggaca ggagatcccg ccagccacca
     7621 ttacccttga tgccaaaggt aatgaacagc aggtcaatct cgacaaactc accgtcgcgg
     7681 cgctggaagg gaaaactgaa ctcaaggcgt tgctcgactg gcagcaggcc attagttggc
     7741 gcggtgagct aacgcttaac ggcattaaca ccgccaaaga gatcccagag tggccgtcga
     7801 aactcaatgg cttgattaaa acccgcggta gcctgtacgg cggcacctgg cagatggagg
     7861 tgccagaact gaagctgacc ggtaacgtca aacagaacaa agtgaacgtt gacggcacgc
     7921 tgaaaggcaa cagttatatg cagtggatga tccctggact gcatctggaa ctcgggccaa
     7981 acagtgccga agtgaaaggc gagctggggg taaaagatct taatcttgat gccaccatca
     8041 acgcgccggg gctggataac gcgctgccgg ggcttggcgg tacagcgaaa gggttggtga
     8101 aagtacgcgg cacggtggaa gcgccacaac tactggcaga tatcaccgcg cgcggcctgc
     8161 gctggcagga actttccgtg gcgcaggttc gcgtggaagg cgacattaaa tccaccgatc
     8221 agatcgccgg gaaactcgac gtacgcgttg agcaaatttc gcagccggat gtaaatatca
     8281 acctcgtcac cctgaatgcc aaaggcagcg aaaagcagca cgagctacag ttgcggattc
     8341 agggcgagcc tgtctccggg cagcttaatc tggcaggaag ttttgatcgc aaagaagaac
     8401 gctggaaggg aactcttagc aatacccgct tccagacgcc ggtcggcccg tggtcgctga
     8461 cccgcgatat tgcgctggat taccgcaata aggagcaaaa aatcagcatc gggccacact
     8521 gctggcttaa cccgaatgcg gaactgtgcg tgccgcaaac tatcgatgcg ggggccgaag
     8581 ggcgtgcggt ggtgaatctc aaccgcttcg acctcgccat gctgaaaccg tttatgccag
     8641 aaaccactca ggccagcggt atcttcacgg gtaaagcgga cgttgcctgg gacaccacca
     8701 aagaggggct gccgcagggc agtatcaccc tttcggggcg taacgtgcag gtaacgcaaa
     8761 ccgtcaacga tgcggcgctg cccgtggcgt ttcagacgct gaatctgacg gcggaattgc
     8821 gtaacaaccg tgccgaattg ggctggacca tccgcctgac caataacggc cagtttgatg
     8881 gacaggtgca ggtgaccgat ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc
     8941 gtaacttcaa ccttgcgatg ataaacccca tctttacccg tggggaaaaa gcagcgggga
     9001 tggtgagtgc caacttgcgt ctgggtggtg atgtgcaaag cccgcagttg tttggtcagc
     9061 ttcaggttac gggtgtggat atcgatggca actttatgcc gtttgatatg caaccgagcc
     9121 agcttgcggt caactttaac ggtatgcgct cgacgcttgc cggtacagta cggacccagc
     9181 agggtgaaat ctacctgaac ggtgatgccg actggagcca aattgaaaac tggcgggcgc
     9241 gggtaacggc gaaaggcagt aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg
     9301 tatcgccaga tgttgtattc gaggctacac caaacctgtt taccctcgat ggtcgcgtgg
     9361 atgtcccgtg ggcgcgcatc gtggtgcacg atctgccgga aagcgcagta ggcgtctcca
     9421 gcgatgtggt gatgcttaac gataacttgc aaccggaaga gccgaaaacg gcgtcgattc
     9481 cgattaacag taacctgatt gtccacgttg gcaacaatgt gcgcattgac gcctttggcc
     9541 tgaaagcgcg gctgacgggc gatcttaacg tcgttcagga caaacaaggg ctgggcctga
     9601 acgggcagat caacatccct gaagggcgct tccatgccta tggtcaggat ctgattgtgc
     9661 gtaaaggtga gttactgttc tctggtccgc cagatcaacc gtatcttaat attgaagcta
     9721 ttcgtaaccc ggatgctaca gaagacgacg taatcgccgg agttcgcgtc actggtctgg
     9781 cggacgaacc gaaagcggag atcttctctg acccggcgat gtcgcaacaa gctgccttgt
     9841 cttatttgct acgtggacaa gggctggaga gcgatcagag cgacagtgcg gcaatgacct
     9901 cgatgctgat tggtctgggg gttgcgcaaa gtggccagat tgtgggtaaa atcggcgaga
     9961 cgtttggcgt aagcaattta gcgctcgaca cccagggagt aggcgactcc tcccaggtag
    10021 tggtcagcgg ctatgtattg ccaggtctgc aagtgaaata cggcgtgggt atatttgact
    10081 ctatagcaac actcacgtta cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt
    10141 ctggtgtaga ccaggcactg gatttgctct atcagttcga gttttagcaa tgcgaatatt
    10201 tgtctacggc agtttacgcc acaaacaagg caacagtcac tggatgacca atgcccagtt
    10261 actgggcgat ttcagtatcg ataactacca gttgtatagc ctgggccact atccaggcgc
    10321 agttccgggg aacggaacgg tacacggtga agtttatcgt attgacaacg ccacgctggc
    10381 cgaacttgat gccttgcgca ccaggggcgg tgaatacgcg cgccagttga ttcagacgcc
    10441 gtacgggagt gcatggatgt acgtttatca acgacccgtc gatggattaa agctaattga
    10501 aagcggcgac tggttagaca gggataagta ataataaaag cacgccacct tcgggtggcg
    10561 ttgttttttg cgagacgact cgcattctgt tttgtaattc cctcaccttt tgcttttctc
    10621 tccgagccgc tttccatatc tattaacgta taaaaaactc tgctggcatt cacaaatgcg
    10681 caggggtaaa acgtttcctc tagcaccgtg agttatactt tgtataactt aaggaggtgc
    10741 agatgcgtat taccataaaa agatggggga acagtgcagg tatggtcatt cccaatatcg
    10801 taatgaaaga acttaactta cagccggggc agagcgtgga agcgcaggtg agcaacaacc
    10861 aactgattct gacacccatc tccaggcgct actcgcttga tgaactgctg gcacagtgtg
    10921 acatgaacgc cgcggaactt agcgagcagg atgtctgggg taaatccacc cctgcgggtg
    10981 acgaaatatg gtaaagaaaa gtgaatttga acggggagac attgtgctgg ttggctttga
    11041 tccagcaagc ggccatgaac agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca
    11101 agcctttaat caactgggaa tgacgctggt ggcccccatt acgcagggcg gaaattttgc
    11161 ccgttatgcc ggatttagcg ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt
    11221 gctggtgaat caggtgcgga tgatggatct acacacccgg ctggcaaagc gtattggtct
    11281 ggctgcggat gaggtggtgg aagaggcgtt attacgcttg caggcggtgg tggaataagt
    11341 gtttatttat cgcgggcata aaaaaaccct tactaaccga agcccggtgt tcagggttat
    11401 tgcgccagaa gaacttattt attctttgcg cgctcgaagg aggcaacgat ttcagcttta
    11461 gcggcatctg cgttttccca accttcaact ttcacccact tgcctttttc gaggtctttg
    11521 tagtgctcga agaagtgagc gatctgtgct ttcagcagtt ctggcagatc gtttacgtct
    11581 ttaatgtgat cgtattcttt gctcagcttg ctgtgcggaa ccgcaaccag tttcgcatct
    11641 tcaccggctt cgtcggtcat tttcagaacg ccaaccggac ggcaacggat cacagaaccc
    11701 ggctgcagcg ggtacggagt cgggaccagt acgtcaaccg ggtcaccgtc cagagacagg
    11761 gtgtggttga tgtaaccgta gttgcatgga tagaacatcg cggtggacat gaagcggtca
    11821 acgaacagtg cgccgctctc tttgtcgatt tcgtatttga tcggatctgc gttagccggg
    11881 atctcaataa caacgtagat gtcttccggc agatctttac ccgcagggac gttgagtaag
    11941 ctcatgtctg tttcctttaa aaatatgtgg caaacaagtg ccgagtatta tagccaactc
    12001 gcgccgaatg tcttcgcttg ttttcgtctt cattttcctt tttcgccaat tttaagacgg
    12061 tatgcagagc aggaaaatcc ataaccgtag ccgcattttt catagtgaga tgaaagcgat
    12121 tacaaacttg tgattaacgt tttatttact tttttgaagt gtgatgtaac gcaatccgtt
    12181 acataacgaa ttgtctatag ttttttcgcg aacatctttt caccaataat aattactccg
    12241 acgaggacaa ccctatgtgg aaacgcttac ttgtagtctc tgcagtctcg gcagccatgt
    12301 cgtctatggc gttggccgct ccattaaccg taggattttc gcaggtcgga tcggaatccg
    12361 gctggcgcgc cgcagaaacc aatgtggcga aaagtgaggc cgaaaaacgc ggaattacgc
    12421 tgaaaattgc cgatggtcag caaaagcagg aaaaccagat taaagcggta cgttccttcg
    12481 ttgcacaagg ggtggatgcg atctttatcg ctccggtggt cgcgacaggt tgggaaccgg
    12541 tattaaaaga ggcgaaagat gccgaaatcc cggtcttctt gctcgaccgt tccatcgatg
    12601 tgaaagacaa atctctctat atgaccaccg ttactgccga caacatcctc gaaggtaagt
    12661 tgatcggtga ctggctggta aaagaagtga atggcaaacc atgcaacgtg gtggagctgc
    12721 agggcaccgt tggggccagc gtcgccattg accgtaagaa aggctttgcc gaagccatta
    12781 agaatgcgcc aaatatcaaa atcatccgct cgcagtcagg tgacttcacc cgtagtaaag
    12841 gcaaagaagt catggagagc tttatcaaag cggaaaacaa cggcaaaaac atctgcatgg
    12901 tttacgccca taacgacgac atggtgattg gtgcaattca ggcaattaaa gaagcgggcc
    12961 tgaaaccggg caaagatatc ctcacgggtt ccattgacgg tgtaccggac atctacaaag
    13021 cgatgatgga tggcgaagcg aacgccagtg ttgaactgac gccgaacatg gcgggacccg
    13081 ctttcgatgc gctggagaaa tacaaaaaag acggcaccat gcctgaaaag ctgacgttaa
    13141 ccaaatccac cctttacctg cctgataccg caaaagaaga gttagagaag aagaaaaata
    13201 tggggtattg agggttgcta tgcctgatgc cgattcgtag gccggataag gcgctcgcgc
    13261 cgcatccggc gatggtgcac tgaagcctga tgcgacgctt accgcgtctt atcatgccta
    13321 ctgggagcac gctttacacc gggggaaacc atgacgaccg accaacacca ggaaatcctc
    13381 cgcaccgaag gattaagtaa atttttcccc ggcgtcaaag cgttagacaa cgttgatttc
    13441 agcctgcgcc gtggcgaaat catggcgctg ctcggtgaaa acggggcggg aaaatcaacg
    13501 ctaatcaaag cattaactgg tgtataccac gccgatcgcg gcaccatctg gctggaaggc
    13561 caggctatct caccgaaaaa taccgcccac gcgcaacaac tcggtattgg caccgtctat
    13621 caggaagtca acctgctacc caatatgtcg gtcgctgata atctatttat aggccgcgaa
    13681 cccaaacgct tcggccttct acgccgcaaa gagatggaaa agcgcgccac cgaactgatg
    13741 gcatcttacg gtttctccct cgacgtgcgc gaaccgctca accgcttttc ggtggcgatg
    13801 cagcaaatcg tcgccatttg tcgggccatc gatctctccg ccaaagtgct gatcctcgat
    13861 gaacccaccg ccagcctcga cactcaggaa gtagagttac tgtttggcct gatgcgtcag
    13921 ttgcgcgatc gcggcgtcag cctgatcttt gtcactcact ttctcgatca agtctatcag
    13981 gtcagtgatc ggatcaccgt cttacgcaac ggcagtttcg taggctgtcg ggaaacgtgc
    14041 gagctaccgc agatcgaact ggtaaaaatg atgctggggc gcgagctgga tacccacgcg
    14101 ctacagcgtg ccgggcgaac attgttgagc gacaaacccg ttgccgcgtt caaaaattac
    14161 ggcaaaaaag gaacgatcgc accgtttgat ctcgaagtac gccccggcga gatcgtcggt
    14221 ctggctggat tgctgggatc aggacgtacc gaaaccgccg aagtgatctt cggtatcaaa
    14281 cctgctgaca gcggcacggc gttgatcaaa ggcaaaccgc aaaacctgcg atcgccacat
    14341 caggcttcgg tactgggcat tggcttctgc ccggaagaca ggaaaaccga tggcatcatc
    14401 gctgccgcct cggtgcggga aaatatcatc cttgctctcc aggcccagcg cggctggcta
    14461 cgtcccattt cccgcaaaga acagcaagag attgccgaac gctttatccg ccagcttggc
    14521 attcgcacac cttcaactga acaaccgatt gaatttctct ccggcggcaa tcagcaaaaa
    14581 gtgttgcttt cacgttggct actgacccga ccgcaatttc tgatcctcga tgagccaacc
    14641 cgcggcattg atgttggtgc ccacgccgag atcatccgcc tgattgaaac gctatgcgcc
    14701 gatggtctgg cgctgctggt gatctcctcc gaactggaag agctggtggg ctatgccgat
    14761 cgggtgatta tcatgcgcga tcgcaaacag gtggcggaga tcccgctggc agagctttcc
    14821 gttccggcga tcatgaacgc cattgcggcg taaggagaac agtgtgatgc ctcaatctct
    14881 cccggacact acgccgccaa aaaggcgctt tcgctggcca acgggaatgc cgcaactggc
    14941 ggcgctattg ctggtgctgc tggttgacag cctggtagcc ccgcatttct ggcaggtagt
    15001 gcttcaggat gggcgtttgt tcggtagccc catagacatt cttaaccgtg cagctcccgt
    15061 tgcgttactg gcgattggca tgacgctggt gatcgccacc ggtgggattg atctctccgt
    15121 aggggcggtg atggctatcg ccggagccac aacggctgcg atgacggtcg cgggattcag
    15181 cctgccgatt gttttgttaa gcgccctggg caccggcatc ctggcgggat tgtggaacgg
    15241 catactggta gcgatcctca aaattcagcc gtttgtcgcc actctgatcc tgatggtcgc
    15301 cgggcgcggc gtggcgcaac tgatcaccgc cgggcagatc gtcacgttta actcgccgga
    15361 tctctcatgg ttcggcagtg gatcgctgtt gttcctgcca acgccggtca ttatcgcggt
    15421 gctgacgctt atcctgttct ggctgttgac ccgcaaaacg gcgctgggga tgtttatcga
    15481 agccgttggt atcaacattc gggcggcaaa aaatgccggg gtaaacacgc gaatcatcgt
    15541 catgctcact tatgtgttga gcgggctgtg tgcggcgatt gcgggcatta tcgtggcggc
    15601 ggatattcgc ggtgccgatg ccaacaacgc tgggttatgg ctggagctgg acgccattct
    15661 cgcggtggtg attggcggcg gatcgctgat gggcgggcgc tttaacctgc tactttcggt
    15721 ggtgggggcg ctgattattc aggggatgaa caccggaatt ttgctttcgg gctttccgcc
    15781 agagatgaac caggtggtga aagcggtggt ggtgctttgc gtgctgattg ttcagtcgca
    15841 acgctttatc agtctgatta aaggagtacg tagccgtgat aaaacgtaat ttgccactga
    15901 tgatcaccat cggcgtcttt gtgttgggtt atctttactg cctgacccag tttcccggtt
    15961 ttgcttccac aagagtgatc tgcaatatcc tgaccgataa cgcctttctg gggataattg
    16021 ccgttggcat gacctttgtg atcctctccg gtgggatcga tctctccgtt ggttcggtga
    16081 tcgcctttac cggcgtgttt ctggcaaaag tgattggcga tttcggcctc tcgccgttgc
    16141 tggcatttcc gctggtgctg gtaatgggct gtgcctttgg cgcatttatg gggcttttga
    16201 tcgacgccct gaagatcccg gcatttatca ttacccttgc agggatgttc tttttgcgcg
    16261 gcgtcagcta tctcgtttcg gaagagtcga tcccgataaa ccatcccatt tatgacacgc
    16321 tctcaagcct tgcgtggaaa atccctggcg gcggtcgctt aagtgcgatg ggactgctga
    16381 tgctggcggt ggtggttatc ggcatattcc tcgcgcatcg tacccgcttt ggtaatcagg
    16441 tgtacgccat tggcggcaat gcgacgtcgg caaacctgat ggggatttcc actcgcagca
    16501 ccactattcg catttatatg ctctctaccg gactggcgac actggcgggg attgtcttct
    16561 cgatttatac ccaggccgga tatgcgctgg cgggcgtagg tgtggaactg gacgctatcg
    16621 cctcagtggt gattggcggt acgcttttga gcggtggcgt tggaacggta ttagggacgc
    16681 tttttggcgt ggcgattcag ggactgattc agacttacat aaactttgat ggcacgctga
    16741 gctcatggtg gacgaaaatc gccatcggta ttttgttgtt tatttttata gcattacagc
    16801 gtgggttaac ggtgctgtgg gagaatcgtc agagttcgcc agtgacaaga gtcaacattg
    16861 cgcagcaata aaaacgcctc tccgtgtgga gaggcgcagg agattacgcg tccgggaact
    16921 cacggataaa gcgttcgaca tcttcaacca tatggtcgtt gccgacaaag aacgaacggc
    16981 gctggtgcag ggtttccggg ataatatcca gaatacgctc tttgccatcg ctcgctttac
    17041 cgcccgcttg ttccgccagg aatgccatcg ggttgcactc atacagcaaa cgcagtttgc
    17101 cgtccgggtg gctggcggtg cttgggtaga gataaatacc gcctttcagt aggttacggt
    17161 ggaaatccgc aaccagtgag ccgatataac gtgaggtata cgggcggttg gtggatttat
    17221 cttcttcctg gcagaattta atgtacttct tcaccccgtt cggaaactta atgtagtttc
    17281 cttcgttgat ggagtaggtt ttgcctttct ccgggaagcg catccgttcc tggcacaggc
    17341 agaaaacgcc gagcgaagga tcgtaagtaa aggcgtgaac accgcatccg gtggtgtaaa
    17401 ccagcatggt agaggagccg tataccacgt aacctgccgc aacttgtttg ttaccaggct
    17461 ggaggaaatc ttcttccgtt accggcgtgc caacaggcgt aacgcggcgg tagatggaga
    17521 aaatggtacc gacagagacg ttaacatcga tgttggacga gccatccagg gggtccatca
    17581 gcaccacgta ttttgcgtgt tcacagcctt caaagacgac aatctcatct tcttcttcag
    17641 aggcaatgcc cgcaacgata tcgcgtgctt tcagtgcggc tttcagtttt tcattagcga
    17701 acaagtcgag tttctgctga acctcgccct gcacgttctc agcaccgctg gcacccagga
    17761 tatcaaccag tcctgctttg ttgatatcgc gatggataat cttggcgccc agttttattg
    17821 ccgacagcaa agcagtgagc tcaccggtag catgagaaaa ctcgtgctgc ttttcgacaa
    17881 taaattcacc taacgttttc ataaaacttt ccctgcaatg tttatggagt aaagcgaccg
    17941 caacaatctt aacaaataat ctcaatgttg cgctcaggtg aatcgcgcca gcaaattacg
    18001 gattatcctg aaatgcgttt ctcacttgcc cgacatatgc gtaaaatgag cggcagatta
    18061 aaaaaggata gtgacgtatg cgcattcata ttttaggaat ttgtggcacg tttatgggcg
    18121 gtctggcgat gctggcgcgc cagttaggcc atgaagtaac gggttcggac gccaatgtgt
    18181 atccgccgat gagcacctta cttgagaagc aaggcattga gctgattcag ggttacgatg
    18241 ccagccagct cgatccgcag ccggatctgg tgattattgg caacgccatg acccgtggaa
    18301 atccgtgtgt ggaagcggta ctggaaaaaa acatccctta tatgtcaggt ccacagtggc
    18361 tgcacgattt tgtgctgcgc gaccgctggg tgctggccgt tgccggtaca cacggcaaaa
    18421 ccaccaccgc gggaatggcg acctggattc tggaacagtg tggttacaaa ccgggctttg
    18481 taatcggcgg tgtgccgggg aactttgagg tttcggcgcg tctgggcgaa agcgacttct
    18541 ttgttatcga agcggatgag tacgactgcg ccttcttcga caaacgctct aaatttgtcc
    18601 attactgccc gcgtacgctg atcctcaaca accttgagtt cgatcacgcc gatatctttg
    18661 acgacctgaa agcgatccag aaacagttcc accatctggt gcgtatcgtt ccggggcagg
    18721 gccgtattat ctggccggaa aatgacatca acctgaaaca gaccatggcg atgggctgct
    18781 ggagcgagca ggagctggtg ggcgagcagg ggcactggca ggcgaaaaag ctgaccaccg
    18841 atgcttccga atgggaagtc ttgctggatg gcgaaaaagt gggcgaagtg aaatggtcgc
    18901 tggtaggcga acataatatg cacaatggcc tgatggcgat tgcagcggct cgccatgttg
    18961 gtgtagcgcc ggcagatgcc gctaacgcgc tgggttcgtt tattaatgct cgtcgccgtc
    19021 tggagttgcg tggtgaagcg aatggcgtca cggtatatga cgattttgcc catcacccga
    19081 cggcgattct ggcaacgctg gcggcgctgc gtggcaaagt tggtggtacg gcgcgcatta
    19141 ttgctgtgct ggagccgcgc tcgaatacca tgaaaatggg gatctgcaaa gacgatttgg
    19201 caccttcatt aggtcgtgcc gatgaagtct tcctgctgca accggcgcat attccgtggc
    19261 aggtggcaga agtggcagaa gcctgcgttc agcctgcaca ctggagtggc gatgtggata
    19321 cgctggcaga tatggtggtg aaaaccgctc agcctggcga ccatattctg gtgatgagca
    19381 acggcggttt tggtgggatc catcagaaac tgctggatgg tctggcgaag aaggcggaag
    19441 ctgcgcagta attcggcctc agccagatat agtgtgcgta ggccggataa ggcgttcacg
    19501 ccgcatccgg catttgagca tagtgcctga tgcgacgctt gatgcgtctt atcaggccta
    19561 caggtgcacc gtatccggca aaccatactc acatcaacaa cgaaaattac ccttcattct
    19621 ccgccagttc gcgcagatac tggaaaatct ggcgtgcgga tttcggcggc ttattccctt
    19681 ctttctcttt cttcgcgtta cggatcagag tacgcagctg ctgacgatcg gcatcgggcc
    19741 acagatttaa tacctcagcg atggcgtcat caccttgatc gatcagacga tcgcgcaggt
    19801 tttccagttt atggaacagc accacctgct ggttgtgacg gtttttcagc ttatccagcg
    19861 cctgacggat cggttctaca tcgcgctggc gcagcatctt accgatgagc tgcaactggc
    19921 ggcggcgacc ttccatctta atacgctggg cgagctcaat agccgcgcgc agatccgcat
    19981 ctaacgggat cttatccagc gcgtttttcc ccagatcaac aatttccgcg ccaaggcgtt
    20041 ttagctcctc ggcatcacgt ttaatttcac ttttactgac ccagataatt tcatcgtctt
    20101 cgtcttcgat gtcatcaccg ggaacgtcgt cgagccagtc ttcgggctgc ttagtcatct
    20161 caggctcctt aaaaaaagag gctaatgtta ccagttaaga tgcgcactga aaaacggttc
    20221 tctgttagac ttcagagaaa ctctctacat tatggcactt gcaatgaaag taatctctca
    20281 agttgaagcg cagcgcaaga ttctggaaga agcagtttcc actgcgctgg agttggcctc
    20341 aggcaaatcg gacggtgcgg aagttgccgt cagcaagacc accggcatta gcgtaagcac
    20401 gcgttatggt gaagtggaga atgttgaatt caatagcgat ggcgcgctgg ggatcactgt
    20461 ttatcaccag aaccgcaaag gtagcgcatc atccaccgat ttaagcccgc aggccattgc
    20521 ccgtactgta caggcggcgc tggatattgc ccgttatacc tcgccagatc cctgtgccgg
    20581 cgtggcagac aaagagctgc tggcctttga cgcaccggat ctcgacttgt tccaccctgc
    20641 ggaagtttcc ccggatgaag ccattgaact ggcggcccgc gcagaacagg cggcattgca
    20701 ggcggacaaa cgcatcacca ataccgaagg tggcagcttt aacagccact acggtgtcaa
    20761 agtttttggc aacagccacg gcatgttgca gggttactgc tcaacgcgtc attcgctctc
    20821 cagctgtgta attgccgaag aaaatggcga tatggagcgt gattacgcct acaccattgg
    20881 tcgtgcgatg agcgatctgc aaacgccaga gtgggttggg gccgactgtg ctcgccgtac
    20941 tttatcgcgc ctgtcaccgc gtaaactctc caccatgaaa gcgccggtca tttttgccaa
    21001 tgaagtggca accgggcttt ttggccatct ggtgggggcg atagcgggtg gatcggttta
    21061 ccgtaaatct acctttctgc tggattcgct gggtaaacaa attctgccgg actggctgac
    21121 cattgaagag catccgcatc tgctgaaagg gctggcgtcg acgccattcg acatcgaagg
    21181 tgtgcgcacc gagcgtcgcg atattattaa agatggcatc ctgactcagt ggctgctgac
    21241 cagctattcg gcgcgaaaac tggggctgaa aagcaccgga catgcgggcg gtattcacaa
    21301 ctggcggatt gccggacaag gtctaagctt cgagcagatg ctcaaagaga tgggcaccgg
    21361 gctggtggtg acggaattga tgggccaggg cgtgagtgca attaccggtg attattcccg
    21421 tggtgcggcg ggtttctggg tagaaaacgg cgaaattcag tatccggtta gcgaaatcac
    21481 catcgcaggt aatttaaaag atatgtggcg caatattgtc accgtcggta acgatattga
    21541 aacacgcagt aatatacagt gtggttctgt gctgttgccg gagatgaaaa tcgccggaca
    21601 gtaaaaaaac tggcgcgacc tgccgcgcta taaactaaaa aattacacaa aatcattcgc
    21661 actgcatcga gacggaaagt gaatgagggc cggggagcgt actcgcagta cgtgacccgg
    21721 tcgaatgagc gtagtcgtcg atgaggcagt gtgaaggatg aagtgtaaat aaaaaaggaa
    21781 gtgagcaatg cgaaaaagcc tgttagctat tcttgcagtc tcctcgttgg tattcagttc
    21841 ggcgtcgttt gctgctgatc ttgaagacaa tatggaaacc ctcaacgaca atttaaaagt
    21901 ggtcgaaaaa gccgataacg cggcgcaagt caaagacgcg ttaacgaaga tgcgcgccgc
    21961 agcgctggat gcgcaaaaag caacgccgcc gaagctcgaa gataaatcac cggacagccc
    22021 ggaaatgaaa gatttccgcc acggtttcga cattctggtc ggtcagattg acgacgcgct
    22081 gaagctggca aatgaaggta aagtaaaaga agcgcaggct gctgcagagc aactgaaaac
    22141 gacccgcaat tcatatcaca agaagtatcg ttaattcctc atttccctgt tgcctgcact
    22201 caggtaacag ggaacttatc atcgcaaatg atgcaccacc tgattgctgc tgccgcgcca
    22261 gatcagggaa gggtctttta aatcctgcac aaatttgccg tcgaccagca cgttaatcaa
    22321 atcaacaacc tgcatttgcg cagcgttgag ttcgtcgagt ttatagcctg tccacaccca
    22381 gatgtcttta cccggacact cggcgcggat gcgttgtacc agtttcagaa tatccggcac
    22441 gttttgcgga tgcagcggat cgccgccgga gagggaaata ccctggcgtt tgatacgagt
    22501 gtcattcaga tcgttaatga tctggtcttc cattgcttta gtaaatggct gaccggaatt
    22561 tacccgccat gtgcttttgt tatagcaacc ggggcattca tgaacacacc cggagacaaa
    22621 cagggtgcag cgagtgccgg gaccgttgac gatgtcgaca ggatagtact gatgataatt
    22681 cattatttcg cctggcttat cagtccgcgt gtaggctgca tctggtaatc aatgcctgat
    22741 gcgacgctgt cgcgtcttat caggcctaca gcaattgcca aatgtaggcc ggataaggcg
    22801 tttacgccgc atccggcatt tgcttagctc agagtgaaga ttaaccgatc tgcccattcc
    22861 ccaaatgttt aacgcggcgc ttaacttctt cctgcttacc ggcgttaaac ggacgtgcat
    22921 ccgggctacc taaatatccg cacacgcggc gagttaccga cacacgggag gcgtcatggt
    22981 taccacattt cgggcaagtg aagcctttgc tggtgcactc gaactcaccg gtaaagccac
    23041 actcgtagca ctcatcaatc ggtgtattgg tgccgtaata cggtacatgc tgatagctgt
    23101 aatcccagac atcttccagc gccttcaggt tgtgctgaat gtttggatac tcgccgtagc
    23161 aaatgaaacc accgttcgcc agcggcgggt aaggcgcttc aaagtcgatc ttgtcgtacg
    23221 ggttcacctt cttctccaca tcgaggtgga aactgttggt gtagtaacct ttatcggtca
    23281 cgcccggcac cacgccaaac tcagcagtat cgagacggca gaagcgatcg cacaggtttt
    23341 cactcggcgt gctgtagaga ctgaaaccat aacccgtttc ttctttccac tgatccactg
    23401 cctgacgcag acgttcaaca atggcgatac ctttcgcgcg aagctgctcg ttgtcgtaaa
    23461 catgctcgcc gccgaacagc gcgttaatgg tttcgtggat gccgatgtaa cccagcgaaa
    23521 tagacgcacg accgtttttg aagatttcag aaacatcatc gtcagcattg agacgcacgc
    23581 cacaagcacc ttccatatag aggatcgggg ccacgcgcgc tttcacgcct tcgagacgag
    23641 cgatacgggt catcagcgcc ttacgtgcca gcaccagacg ttcatccagc agcttccaga
    23701 aggtggcttc atcgcctttt gcttccagag caatacgcgg caggttcagg ctgatcacgc
    23761 cgaggttgtt acgaccatcg tggatctgct cgccgttttc attttcccac acgccgagga
    23821 agctgcggca gcccatcggg gttttaaacg aaccggtgac tttcactacc tgatcgtagt
    23881 tcaggatatc cggatacatg cgcttgcttg cgcactccag cgccagctgt ttgatgtcgt
    23941 agttcggatc gcctttttta tggttcaggc catcgcgaat cgcaaacacc agtttcggga
    24001 acaccgcagt tttacggttt ttaccgaggc ctgcgatacg gttacgcagg atagattcct
    24061 gaatcaggcg cgactcccag ctggtgccca gaccaaaacc aaaggttaca aacggcgtct
    24121 gaccgttggc ggtgtgcagg gtgtttactt cgtactccag cgactggaag gcatcgtagc
    24181 actctttaat ggtacgagag ttagcgtagc cttcggcgtc cgggatgttc cactcttctg
    24241 cggtcttgcg atgtttgttg tagctggcgg tgacaaacgg tgccagcact tcatcgatac
    24301 ggttaatggt ggtgccgcca taaatatggc tggcaacctg agcaataatc tgcgcagtta
    24361 cagcggttgc cgtagagatc gacttcggcg gttcaatctc ggcgttcccc atcttaaagc
    24421 cctgggtcag catgcctttc aggtcgatca acatgcagtt gaacatcggg aagaacggcg
    24481 agtaatcgag atcgtgatag tgaatatcgc cacgctcatg tgcctgcacc acgtcacgcg
    24541 gcagcaggtg ctgacgtgca tagtgtttag ccacgatccc ggccagcagg tcgcgctggg
    24601 ttggaatcac cttgctgtct ttgttggcat tttcgttgag taacgaggcg ttggtctgct
    24661 cgaccagacc acggatctcc tgattcaggc gaccgcgttt ttcacgttca atgtcgcgat
    24721 cgtgacggta ctcgatgtaa gcacgagcca gttgtttgta tggacccgac atcagctggt
    24781 tttcaactgc ggtctggatc tcgttgatat ccacctggtt gcggccctgc atctgctcgc
    24841 tgacaaccgc ggcaacagtg gcgcaataat cggcatcatc gacttccgct gctttagctg
    24901 cacgcagaat cgcttctttg atgcgctctg atttaaacgg cactttgcag ccgtctcgtt
    24961 tcatcacatg cggtgtcatg atcgctccat atttttaaga acaggttatc cacagaaatt
    25021 gggaaagacg ttttccggtt ttttcgtttc gttttccggt gctttccgca atcgccatcc
    25081 gcttatccac aacgccacac ctttttcgtg gggagctgta gtagcaatta tagtcgatta
    25141 atacaacata ttgggttggg acgcatttta aagtctatat atagtgcttt gcatcaagga
    25201 tgtttgaact ttttttgatg tagctcaaag taaaaagcag agcgtacgga tgacgggcgc
    25261 tacagcgtta tgtaaatttt ttaatgaatt tgctggttga aaaatcaaca aaaacaacat
    25321 gctgacagac aaaaccccgg aatgaccggc attaccgggg cttagggaag atttacttct
    25381 gtaaccacca gacagcctca aaaggccgta aattcatggc acagggttgt ggtgaggctt
    25441 cttcgtagtt atgcatcaca agctgccagt tgccgcgcat ttgccctggc tgccagggtt
    25501 ggatctcacg gctaaggttg gcaatgacca gcaaggtttg ccccttccat tcacggcgat
    25561 agcaccacaa tacagggctg tttggcagca gatcctggta attgccccat gtcaggatgg
    25621 cttcctgctt acgcagtgcg attaactttt ggtaggtgta aaacaccgag gaatcatcgg
    25681 ccagcgcggc ttctacgttg atttgttgat agttatcgcc caggccaatc cacggttcgc
    25741 cagccgtaaa cccggcatta tcgccgttgc tccattgcat gggcgtgcgg ctgttgtcac
    25801 gggatttact ggcgaggatt gccaataact cgtcggcatc acgcccatcg ttgcgcagct
    25861 cggcaaacat attgaggctc tctacgtcgc gatagtcagt aatgcgcgtg aaatgcgggt
    25921 tggtcatgcc aatctcttcg ccctggtaga tatacggcgt tccctgcatg ccatgcagca
    25981 ccatcgccag catttttgcc gcaggcacgc ggtattcacc ttcatcacca aagcgagaaa
    26041 caatgcgcgg ctgatcgtgg ttacaccaga acaaggcatt ccatgccacg ttgtgcattc
    26101 cttgttgcca gtggcggaac aattttttca acgccacaaa gtcaggttta gccagcgtcc
    26161 atttttcacc accgggataa tcgaccttca ggtgatgaaa attaaaggtc atcgacaatt
    26221 cactgcctgt cagagccgcg tatcgctggc aatgctcaag gctggtggag gacatttcac
    26281 ctacggtcat taacccgcgt ggcgtaaaca catcgcggtt catctcgtgc aaaaactcgt
    26341 gtgctcgtgg cccgtcggtg tagaagcgac gcccgtcgcc atccaggtca tcagggaaac
    26401 gcgggtcttt ggagatcaga ttcaccacat ccaggcgcaa cccgtcgacc ccacgatcgg
    26461 cccagaactc acagactttt ttcagctctg cgcgtaccgc tggattctcc cagttgagat
    26521 ccgcttgttc tggtgcaaag agatgcaaat agtactgttc gctttccgca tgccagcgcc
    26581 acgcactacc gccaaatttt gaacgccagt tgttcggtgg cgtttctggt tcgccatcgc
    26641 gccagatata aaactggcgg taagggcttt ctttgttcag cgcctcgcga aaccaggcat
    26701 gttgggtaga ggtatggtta aacaccatat cgagaatgat acgaatcccg cgcgattttg
    26761 cctgcgtcac cagttcgtca aaatcgtcca gcgtgccgta ggtgggatca atcgccgtat
    26821 agttcgctac atcgtaaccg ttatcgacct gcggagagac ataaaagggg gttagccaga
    26881 tggcatcaac gcccagttta tgcagatagt ccaggcgttg gataacgcca cgtaaatcgc
    26941 cggtgccgct acccgtggtg tcctgaaaac tctttggata aatctggtag ataacgccgt
    27001 tttgccacca gtggggaaga ttagtcatta cgttattcct gcaaatgcga gggggcgcaa
    27061 ttgcgccccg aagaaaatta aacaatgtcc agcgtgccca ggcggtattt ccgctgatag
    27121 ataaacgagg tgagtacaat cgggatgatg atggcgatag ccattgccag cgcaaacacc
    27181 tgccagtagc tcggttgaat cgagagaatt cccggcaggc cgcctacgcc gatgccattc
    27241 gccataacgc cgttcaggcc gcatagcaat cctgccagac cagaaccaat catcgcgcac
    27301 agcatcggga agcgatattt caggttgatg ccgtacattg caggctcagt gaccccaagc
    27361 caggcggaga tcgccgcagg cacggagatc tcgcgttcat tgtgcttgcg gctggaaatg
    27421 ataatgccta tcacggcgga gccctgagcg atattcgaca gcgcaatcag cggccacact
    27481 ggcgtaccac ccatgctttg aatcatctgc aaatcaatag caagcgtggt ctggtgtaca
    27541 ccggtgatca ccagcggggc gtacaggaag ccaaacaatg ctgcgccaat cggagcaaag
    27601 ctgccggtca tcaggtgacg taccgcaaag gcaacaccat cgccaatcat gcgaccaaac
    27661 ggaccaatca gcgcatgggc gaggaacacc gcgaggatca gcgaacagac gggcaccacc
    27721 accagataga ggtaatctgg cacgatgcgt ttaaggcgag tttcaataac gcccagcgcc
    27781 agcccggcta acagtgccgg gatcacctgc gcctgatagc ccactttggc gatgctgaac
    27841 atgccaaagt cccacacttc cggcagctgc tgcccgagca gataagcgtt catcagctgt
    27901 ggagaaacca gtgtcacgcc aagcacgata ccgaggatcg gcgtgccgcc catttttttc
    27961 accgctgacc agcaaatccc gaccggcagg tagaagaaga tcgcttcacc gatcaaccac
    28021 agaaaatcgt agatcgtttg cagggaaggg tacatttgtg ccagcgtttg accgttgctc
    28081 atgggcaaat cgccgatcac attgcgaaaa ccgaggatca aaccgccgct aatcaacgcg
    28141 ggcagcaacg ggaagaagat ctctgcgaaa tgagagatca actgctcatg ccatttcata
    28201 ttctgccggg cggctttttt tacctgctct ttatcaacct gcgcctgtcc ggttgacgct
    28261 atcagtgctt gatagtaatc acccacgttg gtgccaatca ccacctgaaa ttgcccggca
    28321 ttggtgaaac agcctttcac catggggagt tgctcaattt ctttcggtct ggcattggcc
    28381 ggttggttga ggacaaagcg caggcgagta atacagtggc tcaccgtcgc aatattgccg
    28441 cgcccgccga ccagttcaat caaccgatcg atatccgttt ggtttatttt gctcatcata
    28501 aagccccatg gcagatgaca tttttggttg gctgcagaat atattgcgcg gaaaaattta
    28561 aaaacgggaa cgttcccgaa acgcagcgaa gatcacaatt tatcgttcag gaaacgatca
    28621 ggacagggtg gcggggatga tgatttgttg cggttcgctg cgcccggtta cctgtgcgat
    28681 caactggcag gccgcctggc gtccagcttc ggcgtaaccg ggatctacgg tgacgatctc
    28741 cggatggagg aatttcatta acggcgtatt accgacgctc gccagttgca aggtgtcgat
    28801 gcgttgctct tgcaggtatt tacttgcgcc aagtgccagc gtgtcggttg cgcacagtaa
    28861 ggcggtagtt tcaggcgtaa tcacttttgc aacgttctca tagccttgct tcatagcaag
    28921 ccctggcagg gcggcaacgg gatgcagttt atgcgctttg cagaacgcca ggtaggcttc
    28981 gtgacgtcgc ttaccggttg tcacgtcact gtgcggcacg ccgagataac tgatattacg
    29041 atgcccctgg tcatacagcc gttgcatcag gatcttgatt gccccttcgt cgtcataaca
    29101 gaccgaagca aagccttttg cgtcacgcgc cagcagaacc agcgatgact gccagtgggc
    29161 taacatttct tctgttatgc cagtaaaacc gaacagcact acgccgtcga tattacgccg
    29221 tttcagcacc cccaaatgtt cggcaactaa ttgcggggaa aactgacttt ccatcatgat
    29281 tggatcgtaa ccttgttcat agaacgctgg cagcatggtt tgaacggcga gattttctga
    29341 caacgaatcc agacgggtaa caatgatggc gaccacttta tcgctttgcc cacgcatagc
    29401 gcgcgcagag cgggaagggg aaaatccatg ctgattcatc actgcttcaa cacgctcgcg
    29461 ggtgcgctgg ctcacgccgc tttcgttatt cagcacccgg gaaactgtag atttccccac
    29521 gccgcttaag cgcgcgatgt ctttgatggt cagccgattt tgcatcctgt tgtcctgtaa
    29581 cgtgttgttt aattatttga gcctaacttt acccgtgcat tcagcaatgg gtaaagtctg
    29641 gtttatcgtt ggtttagttg ccagcaggta ttatatcgcc atagatgcta cgaatattat
    29701 tggattctcc ttattatttg cggcgctttt ttcacttacc ggaggttata tggaacctga
    29761 tcccacgcct ctccctcgac ggagattaaa acttttccgg taagcccgtc ttttcacggc
    29821 gttaccggat gcgtaaggcc gtgacgtttt aacgtccctg ctcagcttta ttaccttcag
    29881 gtaaggcttc gccacgcctg aagacatttc tgtactgttt cagacagtgc ggagggactc
    29941 cttatgttta aagaaatttt tacccggctc attcgccatt taccttcccg tctggttcat
    30001 cgtgatccat tgcctggcgc gcagcagaca gtgaatacgg tggtcccgcc gtccttaagt
    30061 gcgcattgcc tgaaaatggc ggtgatgccc gaagaagaat tgtggaaaac gttcgacacc
    30121 catccggaag ggttaaatca ggcggaagtg gaatctgccc gcgaacaaca tggtgaaaat
    30181 aaattacccg cacaacaacc gtcgccgtgg tgggtacatt tatgggtctg ctatcgcaac
    30241 ccctttaata ttttactcac cattctcggc gctatttctt acgccacgga agatttattt
    30301 gctgcgggcg ttatcgcgct aatggtcgct atttcaacgt tgctgaactt tattcaggaa
    30361 gcacgttcca ctaaagcggc agatgccctg aaagcgatgg tcagcaatac cgcgacggtg
    30421 ctgcgcgtaa ttaacgacaa aggcgaaaat ggctggctgg agatcccgat cgaccagctg
    30481 gtgcccggcg atattataaa actggcggcg ggggatatga tcccggcaga tttacgtatc
    30541 ttgcaagcgc gggatctgtt cgtcgctcag gcgtcgttaa ccggtgagtc tctgcccgtg
    30601 gaaaaagccg ctaccactcg ccagccggag cacagcaatc cgctggagtg cgacacgctg
    30661 tgttttatgg gcaccaccgt ggtgagcggc acggcacaag caatggtgat tgctacaggt
    30721 gccaatacct ggtttggtca actggcgggg cgtgttagtg agcaggaaag cgagccgaat
    30781 gcctttcagc aagggatcag ccgcgtcagt atgctgctga ttcgctttat gctggtgatg
    30841 gcaccggtgg tgctgttaat caatggttac accaaaggcg actggtggga agcggcgctg
    30901 tttgcgcttt cggtcgcggt aggcctaacg ccggaaatgt tgccgatgat tgttacctcg
    30961 acactggcgc gcggcgcagt aaagctgtcg aaacagaaag tgatcgtcaa acatctggat
    31021 gctattcaga actttggcgc aatggatatt ctgtgcactg ataaaaccgg caccctgaca
    31081 caggataaaa ttgtgctgga gaatcatacc gatatctccg gtaaaaccag cgaacgcgtg
    31141 ctgcatagcg cgtggttgaa cagtcattat cagaccggac ttaaaaacct gctcgataca
    31201 gcggtgctcg aaggtacgga tgaagagtca gcgcgctcgc tggccagtcg ttggcaaaaa
    31261 atcgatgaga ttccgtttga tttcgagcgt cgccggatgt cggtggtagt ggcagaaaat
    31321 accgagcacc atcagctggt ttgcaaaggc gcattgcagg aaatcctcaa tgtgtgttcg
    31381 caagtgcgtc acaatggcga gattgtaccg ctcgatgaca ccatgctgcg taagattaag
    31441 cgggttactg atacgctgaa tcgtcagggg ctgcgcgtgg ttgcggtggc gacgaaatac
    31501 ctgccagcgc gtgaaggaga ttaccagcgg gcggatgaat ccgacctgat cctcgaagga
    31561 tatattgctt tccttgatcc gccaaaagag acaaccgctc cggcactgaa ggcattaaaa
    31621 gcgagtggaa ttaccgtaaa aatccttact ggcgacagtg agttagttgc agcgaaagtg
    31681 tgccatgaag tggggctgga cgcgggagag gtggtcattg gtagtgatat tgaaacgcta
    31741 tctgacgacg aactggcaaa tcttgcacag cgcaccacgc tgtttgcccg cctgacgccg
    31801 atgcataaag aacgcattgt gaccttactg aagcgcgaag ggcatgtggt tggctttatg
    31861 ggcgatggta ttaatgatgc gcccgcctta cgcgctgcgg atatcggcat ttctgtggac
    31921 ggcgcggtag atattgcccg tgaagcggct gatatcatcc tgctggaaaa aagcctgatg
    31981 gtgctggaag agggggttat tgagggacgt cgcactttcg ccaacatgct gaaatacatc
    32041 aaaatgacgg cgagttctaa cttcggtaat gtgttcagcg tgctggtggc gagtgctttc
    32101 ttgcccttcc tgccgatgtt gccgttacac ttgcttattc agaacctgct gtacgatgtg
    32161 tcacaggtgg cgatcccgtt tgataacgtc gacgacgagc aaattcaaaa gccgcagcgt
    32221 tggaatccgg cggatctggg gcgctttatg atcttcttcg gaccgatcag ctcgatcttc
    32281 gatattttga cgttttgcct gatgtggtgg gtattccatg ccaacacgcc ggaaacgcaa
    32341 acgctgttcc agtcgggatg gtttgtggtg ggcttactgt cgcaaacgct gattgtgcat
    32401 atgatccgca cccgccgtgt gccgtttatt cagagctgtg catcgtggcc gttaatgatc
    32461 atgaccgtga tcgtgatgat tgtcgggatc gcattgccgt tttcaccgct ggccagttat
    32521 ctgcaattac aggcgctgcc gttaagctat ttcccgtggc tggttgcgat tctggcaggg
    32581 tatatgacat taacccagtt ggtgaaaggg ttctatagcc gtcgttacgg ctggcaataa
    32641 agaataaact gggcacgata gccccgattc agattgcaga caaagtaagc tttgttcatg
    32701 ccggatgcgg cgtaaatgcc ttatccggcc aacaaattca ataaattgca ggaatcacgt
    32761 aggcctgata agcgtagcgc atcaggcgat tttgcgtttg tcatcagact taatccgggc
    32821 atgatagccc ggatttccat caagattagc gacgaacagc gatcgcttcg atctcaatct
    32881 tcacgtcttt cggcagacgg gcaacttcaa cgcaagaacg tgccgggaag gtggcgttgt
    32941 gttcggtgaa gaaggcttcg taagtggcgt ttacggttgc gaagtcgttc agatctttca
    33001 caaacacggt ggttttaacg atgtcgccca ctttcaggcc agcggcttcg acgatcgctt
    33061 ttacgttatc cagcgactga cgtgcctgtg cagcgacgtc tgccggtact tcgcccgttt
    33121 tcggatttac cgggatctga ccggaggtga tgatcatatt gcccagatca acgccctgta
    33181 cgtaaggacc gatagctgcc ggtgcatttt ccgtcgcgat agttttgctc atgatttctc
    33241 ctttattaca gcggtaaaaa gtctggctca ttatagggag ccagactttt attaccaacc
    33301 gcaattaatt ggccagcacc acattatggg aaaactcttt ttcacagtat ttgcatttga
    33361 gcgcgatatc attggcgcgt tttcgcacgg caaagctgga agaaaccggt tcggcatggc
    33421 tgatacagtt gctgttcggg cagaccagca cattgtcgat gcgctccggc agacttgggc
    33481 gcgatttacc caccacttca tagttgtcga tacggttaac cgtggcttgc ggcgcataca
    33541 atgccagttg atctacctgg tcttcactca aaaaggtatt ttcgattttg atcagatctt
    33601 tgcggcccat ctcgccagaa ggcaggttca gaccgatggt gatgcgctga tccgtttcgg
    33661 tcagcttgaa cagactcaac agcttaaaac cgatctgggc ggggatatgg tcaattaccg
    33721 tgccgcgttt aatagcttca acctgcaatt tattatcgtg tgtcatctct atttcccctt
    33781 acagtaccag atcgcgattc agaaccagtg ccagtaacgc ctggcgagcg aaaatcccgt
    33841 tgcctgcctg ctggaagtac caggcgtgtg gcgttttatc aacatccgtc gcaatctcat
    33901 caacacgcgg cagcggatgc agcactttca tattggcttt ggcgttgtgg agatcgctgg
    33961 cgcgaagaac aaactgcgct ttcacgttgg cgtactcgga cgggtccaga cgctcttttt
    34021 gcacgcgggt catgtacagg atgtcaactt ccgccatcac ttcttcaata gagctgtgca
    34081 gactccaggc gatccctttt tcatcaagca tatccagaat gtattgcggc attgccagcg
    34141 cgtccggcgc gatgaagtaa aaacggttgc cgtcgaactt cgctaacgcc tgagtcaggg
    34201 agtgaacggt gcggccatat ttcaggtcac caaccattgc gacgtggaga ttgtccagac
    34261 gcccctgggt ttcctgaata gtgaataagt ccagcaaggt ttgcgtcgga tgttggttgg
    34321 agccatcacc ggcattcagt accggtacat tgccggaaaa ctcggtggcc aggcgcgccg
    34381 caccttcctg cggatgacgc atcactatcg catcaacgta agtgctgata accgaaatgg
    34441 tatcggccag cgtttcgccc tttttaccca gtgatgtatt ggcgctgtcg gaaaagccca
    34501 ccacgctggc acccaggcgg tgcatggaag tttcgaaaga gaggcgggta cgggtagagg
    34561 cttcgaagaa acagctggca atgactttgt gcttcaacag ctctggttgc gggtttgctt
    34621 tcagtttcgc cgctgtcgcc agcaccagat taaggtcatc gcgactaagg tcgtttatgg
    34681 aaatgatatg tttctgatat agcggattag ccatctttta tctcctgacg cctgggcaaa
    34741 aaaaagcccc tcgattgagg ggctgggaat gggtgatcaa cgggaagaaa aacggcaggc
    34801 cagcgtcttt tttcagacgc ggtaagacaa aatgtcgaac atactgaacc atacatcctc
    34861 ccggcaaatt gtccggcatt atactcatcg tcagaagcgg tgcaagcatt tgatgcaata
    34921 ttttgtcagc gcaaacggtt tatatgaatt taaagtcaaa atatatgcat ttttatttac
    34981 tatgattctg gaggggaact gtcgttcggt atcaataatt gcagacattt cctgcttttt
    35041 tctttttcac tttcacgcaa tcagatatgc attttattcc attcatccga cttatagggc
    35101 ggagtgtgaa aacgaacggc taacactatt gcttacagct cagggatgcg cgctatcact
    35161 ttaatttcaa aatcaaagcc tgccagccat gtaacgccca ccgccgtcca gtttggataa
    35221 ggtggggcgc taaatatttc atttttcacc gtcatgatgt cttcgaattg tttttctgga
    35281 tcggtatgaa agctcgtaac atcaatgata ttgtcaaaag tgcatcccgc agcagccagg
    35341 gtcgcatgca aattatcaaa tgccagtctg acttgttgct gaaaatcggg ttctggtgat
    35401 ccgtcctctc gacttcctac ttgcccggaa acaaacagca aatcgccgga acgaatagcc
    35461 gcagaataac gatgttcagc atacagtgaa tgtcggccag cagggaaaac agcggttctt
    35521 tctaccattt ggttatcctc aagatttacg acttgaacag aagatttata tttaccagga
    35581 gccgctttta gcggacgacg tgagtaaaca aaacccagac atcatgggaa atggctgggc
    35641 ttaattgagc gtagtcggtt atgcgccaaa cgcgccatca atggtatgca tcgcgccggt
    35701 aacaaaactg gcttctggcc ctgctaacca tgcgaccata ccagcgacct cttccggttg
    35761 cccatgtctt ttgatagcca taaaaccatg caacatatcg cgcattggcc cgttggcggg
    35821 attagcgtcg gtatcaattg gccctggctg gacgacgttg atggtgatcc cacgcggtcc
    35881 aaaatcacgg gccagcccgc gcgccatgcc ttgcagggca gatttactgg cggcataagc
    35941 agccatgcct gcaacaggca tacgatcgcc attcacggag ccgatgatta agatgcgccc
    36001 gccttcgggc atctgcctgg cggcttcaac agaggcatgg taaggagcat gaatattgat
    36061 tttgaaaagg cgatcaatat cgtcggcatt taattccagg gcatcgccaa agacgccaat
    36121 acctgcattt accaccagga tatccaatgc gccgctctta cgaacgacat caataacagc
    36181 gtctctgtca gcactatctg tgaatactgc tgtcgctcca gtctcttgtg ccaggcgttc
    36241 agcggcatct ttcgaccccg cataggtgaa tcgtacattg gccccatcgg tgacgaaacg
    36301 acgtacgata gcggcaccga taccacgact gccaccgagg atgagaactg tcttacctgt
    36361 aaaagcgccc ataaggactc cttgatttat tatgtaacat gcattacaaa actgttttaa
    36421 ctttctgtca acaagttttg taatggtcac taaaaaacaa tctcgcgttc caggtcgtcc
    36481 cagacgtttc gctcctgagc aggcagtctc tgcggcaaaa gtgctttttc accaaaaagg
    36541 tttcgatgct gtcagtgtta ctgaagttac tgattatctt ggtattaacc ccccgagcct
    36601 ctacgcggct tttggcaata aggctgggtt atttagccgt gtactcaatg aatatgtcgg
    36661 tacggaagct attccgcttg ccgatattct tcgtgatgat cgtccggtag gggagtgcct
    36721 ggctgaggta ttaaaagaag cggcgcgcag atatagccaa aacggcggct gcgctggctg
    36781 tatggttctt gaaggcattc atagtcatga tccacaagcg cgtgatattg ccgttcaata
    36841 ttatcacgcc gcagaaacgg ccatttatga ctatatcgcc aggcggcatc cacaaagcgc
    36901 acaatgtgtg actgatttta tgagtaccgt gatgtcaggg ctttctgcga aggcacggga
    36961 ggggcactcc atcgaacaac tctgtgcaac agctgcactg gcgggggaag cgataaaaac
    37021 tattctcaag gagtgatgcg ggccttgacc cgaaaggcgg gaacgcgcct gccgataagt
    37081 tgtgataaga caataattca cgcgttaagg ctagcggaat tgatcatctt ttcgtataac
    37141 gatagaaatg aaacgttgtt ttaattaagg agtggaaaag atgatcatcg gaaatattca
    37201 taatcttcag ccgtggctac cccaggagtt acgccaggcg attgagcata tcaaagcaca
    37261 cgttacggta gaaacgccaa agggcaagca cgatatcgaa ggcaatcgtc tgttttatct
    37321 tatctcggaa gatatgaccg agccgtacga agctcgccgt gcggagtacc atgcccgcta
    37381 tctcgacatt cagattgtgt taaaaggtca ggaaggcatg accttcagca cacaacctgc
    37441 aggtacgccg gataccgact ggttagctga taaagacatc gcatttttgc cggaaggcgt
    37501 tgatgagaaa acagttatcc ttaatgaagg tgattttgtt gtgttttatc cgggggaagt
    37561 gcataaaccg ctgtgcgcag tgggtgcacc agcccaggtt cgcaaagcag tagtgaagat
    37621 gctgatggcg tgatgacgtt tcgccgtaaa taactccagg tttacggcga gtttgtgaaa
    37681 agagcgtttt ttgatatttt tttgtgagta aaatttgtaa tgcttagacg ttcttattaa
    37741 ctcaaggagt tcgtcatgag caaaatatca gatttgaatt attctcaaca cattacatta
    37801 gccgacaatt ttaaacaaaa aaatgaagcc ttagatacct ggtatgtggg aatgaatgat
    37861 tttgcccgaa ttgtcggggg gcagaatagc agaagcaata ttctttctcc tagagcattt
    37921 ttggagtttt tggcaaagat atttaccctg ggttatgtgg attttagcaa acgctccaac
    37981 gaagcgggta gaaatatgat ggctcatatt gagtcctcat cttattctaa agatactgat
    38041 ggcaatgaaa aaatgaagtt ttacatgaat aatcctgaag gggagcgagc ggatttatct
    38101 aaggtgaaga tagaaattac acttgcctca gcttctacta aagggattcg tgaaggacat
    38161 acagtcatta tatttaaaca atctgatggt tcgactaatc gttatgaagg gaagtctttt
    38221 gaaagaaaag atgatagttc attacacctg attactaaca aggttctggc gtgttaccaa
    38281 cgcgaagcta acaaggaaat agcgcgtcta ttaaataatc a
//
LOCUS       DAPGRN010000037        37932 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPGRN010000037 DAPGRN010000000
VERSION     DAPGRN010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.037"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..2366
                     /gene="pdeF"
                     /locus_tag="RHW33_002034"
     CDS             123..2366
                     /gene="pdeF"
                     /locus_tag="RHW33_002034"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV1645534.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDVKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERIAALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="RHW33_002035"
     CDS             complement(2405..3946)
                     /gene="ppx"
                     /locus_tag="RHW33_002035"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV1645535.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMELGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="RHW33_002036"
     CDS             complement(3951..6017)
                     /gene="ppk1"
                     /locus_tag="RHW33_002036"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV1645536.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6189..6827)
                     /gene="purN"
                     /locus_tag="RHW33_002037"
     CDS             complement(6189..6827)
                     /gene="purN"
                     /locus_tag="RHW33_002037"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV1645537.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6827..7864)
                     /gene="purM"
                     /locus_tag="RHW33_002038"
     CDS             complement(6827..7864)
                     /gene="purM"
                     /locus_tag="RHW33_002038"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV1645538.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8189..8815
                     /gene="upp"
                     /locus_tag="RHW33_002039"
     CDS             8189..8815
                     /gene="upp"
                     /locus_tag="RHW33_002039"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV1645539.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8901..10190
                     /gene="uraA"
                     /locus_tag="RHW33_002040"
     CDS             8901..10190
                     /gene="uraA"
                     /locus_tag="RHW33_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV1645540.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10240..10986
                     /gene="hda"
                     /locus_tag="RHW33_002041"
     CDS             10240..10986
                     /gene="hda"
                     /locus_tag="RHW33_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV1645541.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11124..11483)
                     /gene="arsC"
                     /locus_tag="RHW33_002042"
     CDS             complement(11124..11483)
                     /gene="arsC"
                     /locus_tag="RHW33_002042"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV1645542.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11504..12967)
                     /gene="bepA"
                     /locus_tag="RHW33_002043"
     CDS             complement(11504..12967)
                     /gene="bepA"
                     /locus_tag="RHW33_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV1645543.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13180..14241
                     /locus_tag="RHW33_002044"
     CDS             13180..14241
                     /locus_tag="RHW33_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV1645544.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14279..15127)
                     /locus_tag="RHW33_002045"
     CDS             complement(14279..15127)
                     /locus_tag="RHW33_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV1645545.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15149..17161)
                     /gene="hyfR"
                     /locus_tag="RHW33_002046"
     CDS             complement(15149..17161)
                     /gene="hyfR"
                     /locus_tag="RHW33_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV1645546.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLKKDCDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLIQTLQTRQPTLTHRADDLFLWQRDPLLHLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17191..17604)
                     /gene="hyfJ"
                     /locus_tag="RHW33_002047"
     CDS             complement(17191..17604)
                     /gene="hyfJ"
                     /locus_tag="RHW33_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV1645547.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYKDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(17597..18355)
                     /gene="hyfI"
                     /locus_tag="RHW33_002048"
     CDS             complement(17597..18355)
                     /gene="hyfI"
                     /locus_tag="RHW33_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDV1645548.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIHRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18352..18897)
                     /gene="hyfH"
                     /locus_tag="RHW33_002049"
     CDS             complement(18352..18897)
                     /gene="hyfH"
                     /locus_tag="RHW33_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV1645549.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(18907..20622)
                     /gene="hyfG"
                     /locus_tag="RHW33_002050"
     CDS             complement(18907..20622)
                     /gene="hyfG"
                     /locus_tag="RHW33_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV1645550.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGDSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALRIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20612..22192)
                     /gene="hyfF"
                     /locus_tag="RHW33_002051"
     CDS             complement(20612..22192)
                     /gene="hyfF"
                     /locus_tag="RHW33_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HDV1645551.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHCLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22197..22847)
                     /gene="hyfE"
                     /locus_tag="RHW33_002052"
     CDS             complement(22197..22847)
                     /gene="hyfE"
                     /locus_tag="RHW33_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV1645552.1"
                     /translation="MTGSTIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22859..24298)
                     /gene="hyfD"
                     /locus_tag="RHW33_002053"
     CDS             complement(22859..24298)
                     /gene="hyfD"
                     /locus_tag="RHW33_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HDV1645553.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     SQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSQLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24315..25262)
                     /gene="hyfC"
                     /locus_tag="RHW33_002054"
     CDS             complement(24315..25262)
                     /gene="hyfC"
                     /locus_tag="RHW33_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV1645554.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLSMALPLFIAVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     DSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQEVQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25273..27291)
                     /gene="hyfB"
                     /locus_tag="RHW33_002055"
     CDS             complement(25273..27291)
                     /gene="hyfB"
                     /locus_tag="RHW33_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HDV1645555.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISALPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYSRENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27291..27908)
                     /gene="hyfA"
                     /locus_tag="RHW33_002056"
     CDS             complement(27291..27908)
                     /gene="hyfA"
                     /locus_tag="RHW33_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV1645556.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEKNAPTQHALLRWEPGVQTVAVKCDLCDFLPQGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28161..28631)
                     /gene="bcp"
                     /locus_tag="RHW33_002057"
     CDS             complement(28161..28631)
                     /gene="bcp"
                     /locus_tag="RHW33_002057"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV1645557.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28631..29203)
                     /locus_tag="RHW33_002058"
     CDS             complement(28631..29203)
                     /locus_tag="RHW33_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV1645558.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29349..30227
                     /gene="dapA"
                     /locus_tag="RHW33_002059"
     CDS             29349..30227
                     /gene="dapA"
                     /locus_tag="RHW33_002059"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV1645559.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30244..31278
                     /gene="bamC"
                     /locus_tag="RHW33_002060"
     CDS             30244..31278
                     /gene="bamC"
                     /locus_tag="RHW33_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV1645560.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRAATTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31491..32204
                     /gene="purC"
                     /locus_tag="RHW33_002061"
     CDS             31491..32204
                     /gene="purC"
                     /locus_tag="RHW33_002061"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV1645561.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32372..33235
                     /locus_tag="RHW33_002062"
     CDS             32372..33235
                     /locus_tag="RHW33_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV1645562.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33250..35265
                     /gene="tmcA"
                     /locus_tag="RHW33_002063"
     CDS             33250..35265
                     /gene="tmcA"
                     /locus_tag="RHW33_002063"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV1645563.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALS
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDGQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSNAL
                     VFDDENFTHTPQGNIVISAFEQTLWRIEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDGALSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35339..36037
                     /gene="ypfH"
                     /locus_tag="RHW33_002064"
     CDS             35339..36037
                     /gene="ypfH"
                     /locus_tag="RHW33_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1645564.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAINNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36118..36318)
                     /locus_tag="RHW33_002065"
     CDS             complement(36118..36318)
                     /locus_tag="RHW33_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV1645565.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36346..37473)
                     /gene="dapE"
                     /locus_tag="RHW33_002066"
     CDS             complement(36346..37473)
                     /gene="dapE"
                     /locus_tag="RHW33_002066"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV1645566.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(37477..37833)
                     /locus_tag="RHW33_002067"
     CDS             complement(37477..37833)
                     /locus_tag="RHW33_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV1645567.1"
                     /translation="MVTLYGIKNCDTIKKARRWLETNNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRYKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9220 a         9819 c        10004 g         8889 t
ORIGIN      
        1 actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag
       61 tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat
      121 ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt
      181 tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt
      241 ccggtgaggt ttttctcttt tatctgcctc tggcactgat gatcagcatg atgatgtttt
      301 tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc
      361 ttggttttta cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt
      421 ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt
      481 taatttccca gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt
      541 tccagtttgc tgcgtttgta ggattactgg cgagcagaga aaatctggtc ggcgtcatgc
      601 cttttaacct cgggacctta atcaattatc aggccttact ggtgggcaat ctgattggtg
      661 tcccgctgtg ctacttcatc attcgggtag ttcgaaatcc gttttattta cgtagttatt
      721 attcgcaatt aaaacagcag gttgatgtca aagtcaccaa aaaagagttc gcactctggc
      781 tactggcatt aggtgcttta ctgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa
      841 tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc
      901 gctatggtta taagctgatt tcactgctct gggcggtcgt gttgatgatc agcatccaca
      961 gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct
     1021 caagttatct ggtattctct tttattgtca attatatggc tgtactggca acccgtcaga
     1081 gagcggtagt cagacgcatt caacggcttg cgtatgtgga cccggtggtt catctgccaa
     1141 atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac
     1201 gcatccctgg catggaaatg ctggttaaaa actatggcat catgctgcgg attcaataca
     1261 agcaaaaact ttctcactgg ctgtcaccat tgctggaacc gggtgaagat gtttatcagc
     1321 tttcgggtaa cgatctcgcg ctgcgactga atacagaatc gcaccaggag cgcattgccg
     1381 cactggatag ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc
     1441 agattggcgt cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg
     1501 gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc
     1561 gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac
     1621 tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc
     1681 gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatcg
     1741 gcccggatag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt
     1801 gggtcattga gcatacgctg caatttatgg ctgagaacag agcgaagatg cccgctcacc
     1861 gttttgctat taatctgtct ccaacctcgg tatgccaggc tcgttttcct gttgaagtca
     1921 gtcagctgct ggctaaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa
     1981 gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag
     2041 gctgccagat tgcgattgat gatttcggca ccggctacgc cagctatgcg cggcttaaaa
     2101 atgtgaatgc cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata
     2161 gtctggatta tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg
     2221 tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg
     2281 attatatgca gggttatctt attggtaagc cgcagccgtt aattgatacg ttgaatgaaa
     2341 tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa
     2401 agcattaagc ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca
     2461 cgccttccca gtattcttgc tccttttcca gatcaagcag taccaacgca ttctgactaa
     2521 accagtcatg cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg
     2581 gcggtgtggt ggttgcctga cgttgattgt tgagtaatac gccaaggcgc aatagctgaa
     2641 tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagtttaa
     2701 tcgctttacg gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac
     2761 ccggcaagtc gctgttttgt agaatataag cggagtgacg atgcaaaccg ctgtggttga
     2821 tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat
     2881 gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca
     2941 cccgtcgggc ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac
     3001 tacgcacatc ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg
     3061 ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg
     3121 ccagtcccgg aacgaagact gttttccgct cttcggaaag acctggtaaa ctcagcgatg
     3181 cgaaattacg gtgccgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta
     3241 tcccgtcttt ctcacccaat tccattaaca cttcatgggc ggcttttatg gtaccggaag
     3301 cgcccattgc cacgttccag ccttgaattc gaaattgcca ggttaaagtt tccagttttt
     3361 gtgctgccgc catgcgagca cgctgaaaat tctctttatt gatgactccg ccagggaaat
     3421 aaagctgggc aaagctgaca caccccatcc ggcggctttc aacgagaatc ggttcgaaat
     3481 tttcaccaat caccagttct gtagaaccgc cgccaatatc aataaccagt ttgcgacctt
     3541 tttccggttg ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa
     3601 taatttcaat cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca
     3661 gcgcctgacg cagcgtatgg gtaccaacta tacagacgct ggcaggagaa aacccttgta
     3721 gccgttcggc aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca
     3781 tattatctgg ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct
     3841 gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga
     3901 ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt
     3961 tgttcgagtg atttgatgta gtcataaatc gccaactgcg cccgtacttt gcggcgattg
     4021 ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg
     4081 ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggc
     4141 gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag
     4201 acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca
     4261 acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac
     4321 attccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca
     4381 accaggcctt tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc
     4441 tgcgcgttgg cgatctcgcg gtccaccatt tcatacaaca ggcggcggga gttttgcggc
     4501 gacaccatta aataatcaaa tgtcaccgga cggtatgggt tttcaataaa gttaaatacc
     4561 cgccgtactt cgttggtgat gcgcgcatcg gcggtcagca acgaatagtc agtataaaga
     4621 cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cataacgcac cacttcaccg
     4681 ttttctttac gtgaaatcag aaacagtttg gcgtgaattt tcagccccgg cgcagagaag
     4741 ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtca
     4801 aaacgtgcct gtagctcaac caccacggtg actttcttac cgttgtgtgc ggcgtggatc
     4861 atcgagtcga tgatgcgtga atcttttgcc acacggtaaa tgttgatttt aatcgccagc
     4921 acgctcggat cgaacgaggc ctgacgcagc agttccagca catgctcaaa ggtgtgataa
     4981 ggataataga gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct
     5041 ttatcaaacc aaatatggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg
     5101 acattgggga aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag
     5161 tcgtagcggg aaatagtcag tttttcgcgt aaaacttcaa ccagcgcatt gggcatatcg
     5221 cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc
     5281 aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc
     5341 ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg
     5401 cagtaacgca gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg cgcttctggc
     5461 ggtaaattca caaagcgcgg cactttatct gatgggatct ccagaagcgc gtaacggatg
     5521 gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact
     5581 aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa
     5641 taatgacgca gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc
     5701 tggttgcgcg ccatctccag caatagctcg ttgtagaggc cgtcgaattc ctgatcggct
     5761 ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt
     5821 tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca
     5881 aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc
     5941 gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat
     6001 agcttttcct gacccattac cacttttact ccgttttatt cacgggacac tgcctggata
     6061 ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct
     6121 ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta
     6181 cgggggcatt actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac
     6241 gcggcgtttt cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa
     6301 atggcgtgtt cctgggtttg cacgcgggcg gtgacgtcat cttccgtatc accagcaaat
     6361 accgggactt tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc
     6421 accgatgtac cgtgctcttc atcgccattt tccagcgctt gacgatgggt gtgtaatccg
     6481 ggatatttcg gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca
     6541 aacgccgggc tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg
     6601 tcgatttcat gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggcgatg
     6661 agcgtatgcg ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta
     6721 ttgctgaaaa ctgcccgtac ggtgccttta attttgttgg ttttacaggc gtcaataatt
     6781 gcctgtaaat tacttccgtt gccggaaata agcaccacaa tattcattat tcgataacca
     6841 cgcgttgttc ggaatcagag gctttgatga taccgatttt ccacgcgttt tcaccgttgg
     6901 cattgagcag ggcgagggct ttgtccactt ccggagcagg cagggcgata atcatcccga
     6961 cgccgcagtt gaaggtgcga tacatttcat ggcgctcaac gttacctgcc gtttgcagcc
     7021 agttgaacac ttccggccac tgccaggaag attcatcaat cactgcctgg gtattatctg
     7081 gcaatacgcg cggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca
     7141 catcgacctt ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca
     7201 gatgatcggc taatggctta ccatcaagtt cggtggtttg cggatcacaa ccgctgactt
     7261 caagaatttt gcgcaccagc gaataaccgt tcgagtgcgg accgctggaa ccgagtgcaa
     7321 tcagcacatc gccgtcgctg actttagagc cgtcgatgat ttctgatttt tctaccacgc
     7381 ccacgcagaa acccgcgaca tcgtaatctt caccgtgata catccccggc atttctgccg
     7441 tttcgccacc caccagagaa cagcccgatt gcagacaacc ttccgcaatg ccgctgatca
     7501 ccgctgaagc ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg
     7561 gttccgcacc ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg
     7621 tgtcgtgacg ttttaagtcc attgccagac gcagcttggt acctacgccg tcagtgccgg
     7681 aaaccagcac gggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca
     7741 gaccgcccat cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca
     7801 gagcattacc cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg
     7861 tcactgcttg ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta
     7921 acagggaaag caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttcccc
     7981 aggcgcgcgg cgaaagaaga cttgtgccag ggcaaaggtt agttttcgga tggaataatc
     8041 ttctttcata accatttgaa tataaaataa ctttatctca aactgttatc attttgacta
     8101 aagtcaacga aaataatatt gccgccttga agaaaggagg tataatccgt cgattttttt
     8161 tgtggctgcc cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt
     8221 caaacacaag ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact
     8281 cgcttccgaa gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa
     8341 agtaactatc gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat
     8401 taccgttgtg ccaattctgc gtgcgggtct ggggatgatg gacggtgtgc tggaaaacgt
     8461 tccgagcgcg cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc tggagccggt
     8521 accgtacttc cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc
     8581 aatgctggca accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcaggctg
     8641 cagcagcatc aaagttctgg tgctggtagc tgcgccagaa ggtatcgctg cgctggaaaa
     8701 agcgcatccg gacgtcgaac tgtatactgc atcgattgac cagggactga acgagcacgg
     8761 atacattatt ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat
     8821 aaaaataatt aaagccgact ttatgagtcg gctttttttt gagtaaagcg cctataacac
     8881 ataatacaga ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac
     8941 ttttacagac aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc
     9001 tggtgcccgt cttatttcat attaacccgg cgactgtact gttatttaac ggtattggaa
     9061 cgctgctgta tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg
     9121 cctttatttc accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct
     9181 ttattatgtg cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg
     9241 gctggctgga cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc
     9301 tggagctggc gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc
     9361 cagactccaa aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg
     9421 tgctgttccg tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtggggtacg
     9481 cgctctcttt cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg
     9541 cgctgccaac cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag
     9601 cggcgttagt ggttattgcc gaacacgtag ggcacctggt agtaacggct aatatcgtca
     9661 aaaaagatct gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcttgtcga
     9721 ccgtgatctc cggcttcttt ggctccacgc caaacaccac ctacggggaa aacattggcg
     9781 tgatggcgat cacccgtgtt tacagtacct gggttatcgg cggggcggcg attttcgcta
     9841 tcctgctttc ctgcgtcggt aaactggctg ctgctatcca gatgatccca ttgccggtga
     9901 tggggggggt ttcgctgctg ctttacggtg tcatcggtgc ttccggtatt cgtgtgttga
     9961 tcgaatcgaa agtggattac aacaaagcac agaacctgat cctgacttcc gtgattttga
    10021 tcatcggcgt cagtggcgcg aaggtaaaca ttggcgcggc ggagctgaaa ggtatggcgc
    10081 tggcgaccat cgtcggtatc ggcttaagtt tgattttcaa actgatcagt gtgctgcgcc
    10141 ctgaagaagt ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc
    10201 gggcagtgtt gctgcccggt tcaaacatca tggcattctg tggtaaactt ctcgcgattt
    10261 tgtgaaatcc tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat
    10321 cttcctgacg acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggcc
    10381 gcgctgcaaa acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa
    10441 ggcgcggggc gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat
    10501 gcggtgggct atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt
    10561 atggagcatt tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg
    10621 tgggagatgg cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacggttg
    10681 ttgatcaccg gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg
    10741 cgactcgact gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag
    10801 gcgctacagt tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc
    10861 ttgctgaagc ggctcgacag agaaatgcgc acgctattta tgacgttgga tcagttggat
    10921 cgtgcgtcga ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaag
    10981 ttgtagataa ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa ctacgcgaag
    11041 ttaatccata gtattcgtag gccggataag acgtggcaag cgtcgcatcc ggcaatgtgc
    11101 ttcaactagc gatggagcac atcttaaccc acgatttcca gtacctgctc cggtggacga
    11161 ccaatccgcg ctttgccatt cgcgaccaca atcgggcgct ccatcagctt cggattatcc
    11221 accattgcct gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt cagttcttta
    11281 taaagatcct ctttctggcg catcaattca cgggcactat tcatccccag cattttcagc
    11341 aaatcgcgca gcgttgccgc atcggcgggt gtctcaaggt agagcaccac ttctggctcc
    11401 acgccgtttt ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg
    11461 tagattttta cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag
    11521 gcttaaagcg ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgtgcttgtt
    11581 gcaggctgcc taatttcacc tgcgaactgg cgctactcaa caaggaaata gcctgatcga
    11641 gtcgcccggc gagcgcataa ccttctgctc gtgcagccag ctcctgatcg cggttattta
    11701 gcgcggcttc cgcctgtgcc agtaaatccc agccgttgct gtcatcttta ttattaaagg
    11761 tgtagcgatt gagaatattg gccgcttctt gtggttgacc gccttgcaga taagcgtttg
    11821 ccaggttgag ctgcaacacc ggattggtgc gcaaatcacg ggcatttttc aggcgattga
    11881 ttgcctcatt ggctttgttt tgcccaagat cgatatcggt agccagatcg agataccatg
    11941 cgttgccagg ttctgccgcc agtaacggtt gcagcgtttt tcgcgcctcg tcgtatttat
    12001 tggcttccat cgcctgtaaa gcacgaccat attgcgccgc tcgttgctga cgaacgtttc
    12061 ctttcgccca ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc
    12121 ccagtgtgcg cgctttcgcc agatagaaat cttccgacga ctgcaccacc atcgggcgca
    12181 tctgattagc acggttgcgg gcatctgcca gacgactttc cggcaacggg tgagtcaata
    12241 aaatttccgg cgggcgcgag gagtaacgcg cctgatcgag taatttttcg aggaaggttg
    12301 gcatcgcctg aggatcgaat cccgagcgtt gcagcacctg aataccaatg cggtccgctt
    12361 cctgttcatt ttgctgggtg aaactgatca tcccctgacg cgttcccgcc agtgtaccgg
    12421 tcagcgccgc catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc
    12481 cgacccaggt cagcggcgcg ctgcgctgct gatcttccat cgctcgcgcc aggtgacgtt
    12541 gggtgacgtg ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat
    12601 aacggaacag ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt
    12661 cgtcgttgtt gatcagaaag aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca
    12721 gacgcatccc cagcgaatta atatattgcg ttaacagcgg gtcattaatt aacggcgcgc
    12781 tgccgcgtaa ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg
    12841 tgcttcctgc ggaggttccc atatccggca aggtgtctgc gctgtcggca aacgccgggg
    12901 ctacctgacc aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc
    12961 tgaacataac ctctatcctg tatttctgaa gatagtcatt tgaccgatac tgtgaaatat
    13021 cgttcccacc gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgactttt
    13081 cagtttcctg aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc
    13141 ggatcgcaat cccgctcttg aggttcaggg aaggtcttta tgctcgaaat gttgatgcaa
    13201 tggtatcgcc gccgttttag cgacccggaa gcgattgcct tgctggttat tttagttgcc
    13261 ggatttggca ttatcttttt ctttagtggc ctgcttgctc cgttgctggt ggctattgtg
    13321 ctggcctatt tgctggaatg gccaaccgtg cgcctgcaat ctattggctg ctcccgccgc
    13381 tgggcgacgt cgattgtatt ggtggttttt gtcggtatat tgctactgat ggcgttcgtg
    13441 gtactgccta tcgcctggca acagggcatc tacttaatcc gcgatatgcc ggggatgctc
    13501 aataagcttt ctgactttgc cgccacgttg ccgcgccgct atccggcgtt aatggacgcg
    13561 ggcattattg atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat gggcgattcg
    13621 gtggtgaaaa tttccctcgc ctcgctggtc ggtttgctga ccatagccgt ctatctggtg
    13681 ctggtaccat tgatggtctt cttcctgctg aaagacaaag agcagatgct gaacgccgtt
    13741 cgtcgggtgc tgccgcgtaa ccgtgggctg gcaggacagg tgtggaagga gatgaatcaa
    13801 caaatcacca actatatccg cggcaaagtg ctggagatga tcgtggtggg gatcgccacc
    13861 tggctggggt tcttgctctt tgggctgaac tattcgctgc tgctggcggt gctggtcggc
    13921 ttctcggtac ttattccgta cattggcgca tttgtggtga ccattcctgt ggttggcgtg
    13981 gcgctattcc agtttggtgc aggcacggaa ttctggagct gcttcgcagt gtatctgatt
    14041 attcaggcgc tggacggtaa tctgttagta ccggtgttgt tctccgaagc ggttaacctg
    14101 catccgctgg tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc
    14161 gtattcttcg ccattccatt ggcgacgctg atcaaagccg tgatccacgc ctggcccgat
    14221 ggtcaaatag cgcaagaata atcttcagcc cgacgaactg tttcgccggg ctgatttttc
    14281 aggattcctg acgtaaataa atagcccgat aacatatact caccagcact gcaccgccga
    14341 taatattacc cagcatcacc gggagcagat tggcggtaat aaaatggctt accgtcagtg
    14401 ccggaaaatt gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc
    14461 gaatggcaat ggcgaacgga atcacaaaca aattggcgat acagtgctca aagccactgg
    14521 cgacaaacag ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgcccgc
    14581 aataactcat ccacagcgcc aggcaaacca tcagattgca cataatgccg aggctgacag
    14641 attcagtaaa tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca
    14701 tcgcgttttc actcatcacc agcccggaaa accagattaa caaactgaaa caggcaatac
    14761 ctgccagatt accgcaggcc accagaagtg cgttaatcag ccaagttcgc caactaataa
    14821 cgccccgact ttttgccatc accgtcatta ccgacgaggt gaacaggctg gtgccgcaaa
    14881 ccgccagcaa aataaagccg agtgtaaagc aaaggccgcc caccagatgg gttaatgcct
    14941 gtgaagacgg ggcatcggcg ataacggaaa ggtaaaaagt aaatccgatc gccataaaca
    15001 cgccagcgga catcgccatt aaaaagaccg acactttact gcgggcaatt ttatgggcgg
    15061 caatccgttc agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt
    15121 ttctcatgca tactcctgca aaaacagatt acaacacctc gcgaaccgag atccccagcc
    15181 gctgcattcg tgacagcagc gtggtgcgct tcatccctaa tcgcgtcgct gcgccacggg
    15241 gcccggcaac aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt
    15301 cgtcattctc tggcgtcgtc gggtgcagta actgcgccat tgaactgcga agcgctgaat
    15361 cttcatttag cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt
    15421 taccacgagt caacagtacc gcccgctcaa tcacgttttc cagctcgcgc acgttgcccg
    15481 gccaatccca cgacatcaac tggcgtagcg cctcggtcgg gatggcgtca attgcgcgat
    15541 tcatatggcg cgccattttt tgcgtgaaat gttttgctaa aagagggata tcttccggac
    15601 ggtcgcgtag cggcggcaat tccagtggga agacattcag gcgataaaag agatcgctgc
    15661 gaaactggcg atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga
    15721 cattcaccgg gatcgttcta ctcccgccga gacgctcaat ctcccgctcc tgcaatacgc
    15781 gcagcagttt aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtgc
    15841 cgccatcggc aatttcaaaa cgaccacgat gggtattaat cgcaccagta aacgcccctt
    15901 tgtcatgacc gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt
    15961 tgaccagcgg cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg attacctctt
    16021 tgccagttcc ggtttcaccg caaatcaaca ccgtactgtc gctcttcgcc acaatatcta
    16081 cctgctggag caggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat
    16141 tcgaaaggag ctgctcgcta agctggtggt tttcttgctg caaactttcc tgcaaatcgg
    16201 tcatgctacg ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gctatgtgtt
    16261 gtagtagctg gcagttttcc tcactaaaga gagtggaaga ggtatgcgcc agcaacaatg
    16321 cacccggtgt atggttgcca aaggtaagcg gtataaggag cgcagattcg cagccgttag
    16381 atgcaagtaa gtgtaataac gggtcgcgtt gccagagaaa cagatcgtct gcacggtgcg
    16441 tcaaggtcgg ttgtcgggtt tgtagcgttt gtatcagcaa tacactgtcg ccaggcatat
    16501 tgcgtggcag acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct
    16561 cgttctttcg atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga
    16621 tctcacgggc gacgtcagcg atcagatcgt cgagatcaag atgtgacagc actgcattag
    16681 taatatcaac cagtacccga tgatgatcgc aatctttctt cagcaaacgc agcgccacct
    16741 gctgttcttc atcattgagc catgattgca gaaccggggt gataatgcgt gccagccagt
    16801 taaacagtgt gcaatcctgg ggagtaaaac gactgccgtg ggtgcgaata aagcgtagct
    16861 ccgccattcc actactgccg agaaggacgg cgtgacgcga ataatcgacc atctcgccct
    16921 gatactctac tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagaagttgc
    16981 ttttgagaac atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga
    17041 aggcgcgtaa taaagacgcc ttgccgtgtt tctgcgctaa ccgctccgcg agcattccgt
    17101 ttaccgcttc aattgttatt ccttgcggcg gggcaaacat cgcctcgtct gacatagcca
    17161 tcaacagtcc ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat
    17221 agccggttcc agcacaattt catccaacag atgaatgaat tgaatgctac gcgcttgcca
    17281 cacagacgtc gcgtcatctg ccagtggcgc gaatgcccgg gtcaacaggg cggtgtggct
    17341 ggcgtcaata acaatctcac caaaagtcat cacccccagc atcttacgtc gggcttgctc
    17401 ctcttcgacc agtgcaagcc aatctttgta ttccgtcagt gggcagcgga aggcgacatt
    17461 cagacaatca atcacgccaa cgtgatgacc gatagccagc gagtaataca ttacctggga
    17521 gctgtgttcc ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac
    17581 aatttccccg cactcctcag ccatgtaacc cccggagaac gcgaaagagt tgctgaacga
    17641 tactattgag acgtggatcg tcggcatcgc gcaaccaggt gttaacccga tttcctgtag
    17701 gatcgtcgct taaatggcgc aggagccgat cgcaaatttc tcgcccctga cgatagcccg
    17761 ccagccgccg cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgcggccaca
    17821 acggttgcat agtcaccccg gtgggatcgc gataatccac cgcgtgaatc ttctgttgca
    17881 gcaaaccgag cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg
    17941 ggatccaaac atcaatgggg acaatggtgt cgctgccgcc ccagacgctg tagagatcgt
    18001 ggaaaatacc gccgccgaca ccgcacgcgc cgtaggaaac acagatttta tgatcggggg
    18061 cagactcata cgcccgaagt gcaggcatac gcatcgcccg ggtgactgcg ccagtaaata
    18121 acaaaatatc ggcgtgacgc ggtgatgaaa caaccttaat gccaaaacgt tctgcgtcga
    18181 atactggtgt gatggcggca aaaatttcga tttcacaggc gttgcagccg ccgcaatcga
    18241 cgcgataaac gtaagccgag cgatggatat cctgtagcaa atgccgcttc atcttttgcg
    18301 tttgctcgtc cagcgtgatg ggctggctga catgttgtgt aagcactgga ctcatagctg
    18361 ctccttagcc accagcggta catctgtatc gtcgttgatc agcgtcgcgc gttgtttgca
    18421 ctccgggcag acgctcgctt gcgcccgcaa catttcgcgg ttttgtggcg cattttgttg
    18481 ctgtgccaac aattcagcag ccagtgcgac ggttttttgc ggggcaaacg ggcgttcgca
    18541 acggctgcaa cgttgtagat ggaacgtcgc gcgggtatag agatcggctt tattggtgac
    18601 ggtcagttca aagttattgg taagctggat ggctctggtc gggcacactt cttcacaacg
    18661 tccgcagtaa atacaacgcc ccagatagag ctgccaggtg cgcgaatttt gctggtcgtc
    18721 ggtctggata gtcagcgcat ttgccggaca agcacaggcg caggcaccac aggcaataca
    18781 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
    18841 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
    18901 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
    18961 cggcacggtt tttgactggc gcttgcgcac atcaaccagc gtcacacggt cggtacagga
    19021 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgtag
    19081 catgtaacgc agcaccggcc agttggcgta ggtggcggca cgacagcgcc agcggaacaa
    19141 tttttgatta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
    19201 gccgagtgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagcg gggtatccgg
    19261 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
    19321 catcacgcgg gagaaaacat cgccgccggt gaaggtaaac agtgtttttg gaatattgcc
    19381 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
    19441 cggtcctacg gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
    19501 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
    19561 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
    19621 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
    19681 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaaac caacaaaatg
    19741 gcaggagagg ccaaggttga gcaaatgact gtgtagccgt tcgacttcca gcagaatcga
    19801 gcgaatggta tgcgctcgtt gcggcacctc aatcctcagt gcattttcaa ccgagttggt
    19861 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata agaaggtcac
    19921 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
    19981 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
    20041 atcggaggtg atatgcagcg ggccgacagg gattacccgc gcgtcgctgt cgccctcgtt
    20101 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
    20161 gtctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca ggcgacgctg
    20221 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
    20281 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
    20341 cttcaccacg atccagcatt tttcggcccc ttccatagaa agggcataat aaacggcgta
    20401 atgaccgtta agtgtccgct cgtcgttgcc aaacaggacc ggaagccagc catcatgttg
    20461 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
    20521 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
    20581 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
    20641 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
    20701 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
    20761 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
    20821 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gcagccatcc
    20881 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcagat
    20941 gattacgtgc cagtccggcg gtaacggtca taaattcgct aagaaaaatg ttgaagggcg
    21001 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
    21061 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
    21121 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc agcgcggcaa
    21181 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagtcccatg ttctccacgc
    21241 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
    21301 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggcct
    21361 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
    21421 agagtagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
    21481 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
    21541 ttaatgttgg atcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
    21601 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
    21661 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgcttccagc gatgaacgct
    21721 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
    21781 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
    21841 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgagccact tcatgacgca
    21901 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
    21961 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
    22021 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagcaatgta
    22081 atacggtcac cgtgcgagtc gcagagtgcc cccgtttccg gcaggcaaaa cagagcagcg
    22141 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
    22201 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
    22261 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
    22321 agttccggtg cacgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
    22381 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
    22441 agaaaatgac ctaacgccac cgccagcgct ggtttcagcc cggtagccat cggtagcttc
    22501 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
    22561 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
    22621 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
    22681 tgctctgcgg cgaacaggca cgagagggtg gcgaaaatag acaccagcac cagtgactgg
    22741 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
    22801 agcatcatca gtcccgccag attatttacg atcgtagaac cggtcattca tcatctcctt
    22861 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
    22921 gcaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
    22981 cggggacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
    23041 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccggcag
    23101 caaacagcgg gaatttactg aagaagccgt tgaacggcgg tacgccggta atcgccagcg
    23161 ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca cgcagacgcg
    23221 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
    23281 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc tgcgagccga
    23341 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
    23401 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa tccataaaga atggtgacca
    23461 gggccatgac catgccaaca ccgccaatca catgcgggat attgccgccg tcgataatgg
    23521 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcgctga
    23581 tcggtgttgg cgcttccatt gcgtccggta gccacgcttg catcggtagc tgggccgatt
    23641 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
    23701 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
    23761 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa cgctgtgatt
    23821 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
    23881 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacaaca
    23941 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
    24001 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
    24061 gcgtactcac gcgatcaatg accaggccaa acaatgccac atcccccacg ttgaccaacg
    24121 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
    24181 gcgtggtcag cgcggcgaac aaaacccccc attcggcggc ccgacgttgt ggcgaaaacg
    24241 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
    24301 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
    24361 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
    24421 gcgatagagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    24481 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    24541 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgccagcgc cagcccggca
    24601 ccggaatatt ctgtcagcgg gccttcctgt acttcctgtt ctgcttcagc aacatcaaag
    24661 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    24721 gttagcggcg agttccagcc catcgccagc gtattgctga tcatctcgat atgcgtggaa
    24781 tccgctatca gtgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    24841 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagcccggaa
    24901 agagcaaaga aaaaacgaaa cagggcaaga agataaataa gggtgatcag atcgccgccg
    24961 cccgcaaaag gggaaacggc aataaatagt ggtagggcca tcgacagcac cagcatgctg
    25021 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcaacttcc
    25081 tgccgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg tccgcggcgg
    25141 gagtgcattc gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    25201 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    25261 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    25321 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    25381 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    25441 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    25501 tagacgaaat aacgcagaga agaccacacg cagcggctgc atcacgccat tgcctgacgg
    25561 agccatcgcg ttttccctgc tgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    25621 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    25681 taacagcaac agtaatgagg gggtgacctg tgtatgaaac gtgccgggta caagtgctat
    25741 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatat gcattatctt
    25801 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    25861 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac tgcgcggcgc
    25921 accacagaaa gtaataccgt acatttttac gaagcacatt actgccagcc caccagtgac
    25981 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    26041 tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaag
    26101 ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    26161 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cgcccgcgcc
    26221 gagaaatagc agccctttga acagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    26281 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    26341 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    26401 ttccgccagc gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    26461 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    26521 gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt gcgatggtgc
    26581 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    26641 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    26701 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat
    26761 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga ttgacgtttt
    26821 atcctgcctg gcaatgacca gaaaccagga agacagtgac atcatttcaa acagcacgat
    26881 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    26941 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    27001 gagcgaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    27061 cattcgcagg gttaaatcgg caaagtcaaa aggggtggca aaacgggcga ctaaagttac
    27121 gcctgcatgt aattgcgtaa ttccgctgat aatgccaatc acgccgccca ccagcgatgt
    27181 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    27241 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    27301 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    27361 tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    27421 gggcaagcgc gaacgcaggc cggaccttgt ggcaagaaat cacacaggtc gcatttcacc
    27481 gcgacggtct ggacacccgg ttcccagcgc agcaaagcat gttgtgttgg cgcgtttttt
    27541 tcgccgtctt tgagtgagcc ctcagcttga aaaacatatt gcgcaggggc attcaccgga
    27601 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    27661 atacagaggc tttcgttgag ttggatcgca tcatccctct gggagatggc attgaccggg
    27721 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    27781 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    27841 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    27901 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcataat gcataaagga
    27961 tgccagtatg attaatttaa tatattcaat aagttatgaa ttaactgaat tctgaacggt
    28021 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    28081 aaagtgagaa tgtgatcttg atcacgataa gtgaggtgtg ctgaccgcaa gcgcagccag
    28141 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    28201 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    28261 aagctgatgc gatgaatacc atcgtaggtt ttgcccatga aggacttttc cccccagacg
    28321 ccgaattgct cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    28381 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    28441 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    28501 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    28561 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    28621 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    28681 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    28741 cgtccgcaga tgcggggctg tgggcagtaa tctgaatatg caactgcgca gcccgttcat
    28801 tttcagcagg ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    28861 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    28921 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    28981 gttcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    29041 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    29101 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    29161 ccaacgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    29221 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    29281 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    29341 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    29401 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    29461 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    29521 atgtggtgat gatgacgctg gagctggctg acgggcgcat tccggtgatt gccgggaccg
    29581 gtgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    29641 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    29701 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    29761 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    29821 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    29881 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    29941 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    30001 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    30061 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    30121 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    30181 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    30241 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    30301 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    30361 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    30421 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    30481 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    30541 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    30601 gtggttagcg tgctgcaggc gaaaaactac accattaccc aacgtgatga tgctggtcag
    30661 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    30721 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    30781 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    30841 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    30901 gcgcaaaatc gtgccgccac cactatggac gtacaaagtg cagctgatga caccggttta
    30961 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    31021 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    31081 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    31141 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    31201 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    31261 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    31321 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    31381 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    31441 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    31501 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    31561 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    31621 atcgcaaagg tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    31681 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    31741 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac
    31801 gtcttggaat cgaagaaggt attgagctga acccgccgct gtttgatctg ttcctgaaaa
    31861 acgacgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    31921 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    31981 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggaattt ggtctgtaca
    32041 aaggcgaagt ggtactgggt gatgagttct ccccggacgg tagccgcctg tgggacaaag
    32101 aaacgctgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgagg
    32161 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    32221 cttgccgtgc tgctggcacg gcaagacaac cattctcgta agatgtgcat tgaacgctca
    32281 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    32341 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    32401 caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc ccggtttccg
    32461 tctgccgagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    32521 tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tctcaacagc aatcaacgcg
    32581 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    32641 ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct
    32701 ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca taatggggcc
    32761 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    32821 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    32881 tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    32941 gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    33001 cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg atctggaaga
    33061 ggcgctgaac gctgcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    33121 agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct ggtttaaacg
    33181 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    33241 ggggcaggga tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa
    33301 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    33361 aagttacgtg atgccttatc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    33421 aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg gcatgcggta
    33481 ttcgacgccc gccacggctt tgatgccgct gcctttgccg cacttagcgg aacgttgaaa
    33541 gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat
    33601 gccgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag
    33661 catttcaaac gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg
    33721 ttctcgttgg cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa
    33781 ccagaacaac agcaactctt acagcagcta ctgaccatgc cgtcgggcgt ggcagcggta
    33841 actgctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc tcgtattgcg
    33901 ggtagtgcga ttgtcactgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg
    33961 ggcgagaagt ttcgctttat tgcgccggat gccttgttag ccagcgatgg gcaagccgac
    34021 tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    34081 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt
    34141 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg
    34201 atccgctggg cacagggatg cccgctggaa aaaatggtta gtaatgcact ggtttttgac
    34261 gatgaaaact tcacccatac accacaaggc aatattgtca tttccgcatt tgaacagacg
    34321 ttatggcgaa tcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac
    34381 taccggactt cgccactgga tttacgccgc atgatggatg caccagggca acatttttta
    34441 caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga gggtggatta
    34501 tctcaagaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg
    34561 gcccagtcgc tggcggcgca cggcagcaat ccactggcgg cgacattgcg cggacggcgg
    34621 gtcagccgga ttgcagttca tccggcgcgt cagcgggaag gcacagggcg gcaacttatt
    34681 gctggtgctt tgcaatatac gcaagacctc gactatcttt cggtaagttt tggttacacc
    34741 ggggagttat ggcgtttctg gcaacgctgc ggttttgtgc tggtgcgaat gggtaatcat
    34801 cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag tgatgcgggt
    34861 aaacagctgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag
    34921 tggaatggcg aaatgcttcc tgttgatcca ctaaacgatg gcgccctttc tgacgacgac
    34981 tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc attaggttgc
    35041 ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag
    35101 aaaaacgcca gtgacgcgca attatgtacc acacttaaac tttcaggccg caagatgtta
    35161 ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga tgttcgcact
    35221 gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct
    35281 cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat
    35341 gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct
    35401 tttccacggt gtcggggata acccggtggc aatgggggaa ataggcagct ggtttgcgcc
    35461 gctatttcct gatgcgctgg tggtgagtgt cggaggcgcg gagccgagcg gtaatccggc
    35521 ggggcgtcag tggttttcgg tgcagggtat tacggaagat aatcgccagg cgcgtgtgga
    35581 tgcgatcatg ccgacgttta ttgaaacggt acgctactgg cagaaacaga gtggagtagg
    35641 ggctaacgcc acggcgctaa ttggtttctc acaaggcgcg attatggcgc tggagagcat
    35701 taaagccgaa ccgggccttg cctcacgcgt cattgccttt aacggacgct atgcgagcct
    35761 gccggaaacc gcgtctacag ccacgacaat tcacctgatt catggcggtg aagatccggt
    35821 tatcgatctg gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt
    35881 gacgctggat atcgtggagg atttaggcca tgcgatcaac aatcgcagta tgcaatttgc
    35941 cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg
    36001 taagcctggc gatgatgatg tgattgaaat gatgtaataa tagccgggta ggcctgataa
    36061 gaccgtatca ggcatcatgg ccggatgcgg cgtgaacgcc ttatccggcc actcagctta
    36121 cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc
    36181 acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttatggtcga cgcgcttgag
    36241 atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata
    36301 tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcacg cgacgagctg
    36361 ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc
    36421 attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg
    36481 agcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt
    36541 atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg
    36601 ctgcccggaa agccaccaat ccaccgtata gcgcagttga tgtttttcaa gcagggcaag
    36661 cacctgcgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac
    36721 aaacagttca cccggaataa cgttgttact gcccgtgccc gcctgaatat tggcaatctg
    36781 catactggtc gccgggaaga attcattgcc ttgatcccac tcaatagcca ctaattcatt
    36841 aaggaaaggt gctgcgcgat gtaccggatt gtcagccaga tgtgggtagg caacatgccc
    36901 ctgaacgcca tgaatggtta ggttgcaggt taacgatcca cgacgaccat ttttcaccac
    36961 atcacctacc acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt
    37021 acgtgccatt aacgcttcga cgacttttac cgtaccgttg tgtgcactgg cttcttcatc
    37081 agaggtgatc agaaatgcca gtcgccccgt atggttggga tgttgtgcga caaaacgttc
    37141 tgctgctacc accattgccg ccagcgagcc tttcatatct gccgcaccgc gcccgaataa
    37201 catgccgtca cgaatggtcg gttcaaacgg cggattgatc caacggtcgg cgtcgccagg
    37261 cggcaccacg tcggtatgcc cggcaaaggc caacgtttca ccttgcccac gccatgccca
    37321 aaaattctgc gtatcggcaa aatccatgcg ttcaacggta aaaccgatcg cctgcaaacg
    37381 ttcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag
    37441 ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg
    37501 ataactggaa tcactgaaac ccagcagcat aggcttaccg ggcgcgcaga gcaatggacg
    37561 tttgataatt gcaggcattt cagtcattaa tgccgccgca gaggccgcat cggtgatttt
    37621 atagcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttga gtaacgcttc
    37681 ccagcctaat tcgttgataa aaccgttcaa taattcgctg tccagcccat cgacgcggta
    37741 atcatgaaaa cgatagtcga tgttattggt ttccagccaa cggcgagcct ttttaatggt
    37801 gtcacaattt ttgatgccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa
    37861 taaatactca tttaagagtg aaaacatttt gcctgcaaat gattatcagg aaatataaca
    37921 ttatcgaagt at
//
LOCUS       DAPGRN010000038        36876 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPGRN010000038 DAPGRN010000000
VERSION     DAPGRN010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.038"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..260)
                     /locus_tag="RHW33_002068"
     CDS             complement(<1..260)
                     /locus_tag="RHW33_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645568.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFTINFATSPI"
     gene            783..2711
                     /gene="thrS"
                     /locus_tag="RHW33_002069"
     CDS             783..2711
                     /gene="thrS"
                     /locus_tag="RHW33_002069"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV1645569.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2715..3257
                     /gene="infC"
                     /locus_tag="RHW33_002070"
     CDS             2715..3257
                     /gene="infC"
                     /locus_tag="RHW33_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV1645570.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3354..3551
                     /gene="rpmI"
                     /locus_tag="RHW33_002071"
     CDS             3354..3551
                     /gene="rpmI"
                     /locus_tag="RHW33_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV1645571.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3604..3960
                     /gene="rplT"
                     /locus_tag="RHW33_002072"
     CDS             3604..3960
                     /gene="rplT"
                     /locus_tag="RHW33_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV1645572.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4077..4201
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4083..4127
                     /gene="pheM"
                     /locus_tag="RHW33_002073"
     CDS             4083..4127
                     /gene="pheM"
                     /locus_tag="RHW33_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV1645573.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4410..5393
                     /gene="pheS"
                     /locus_tag="RHW33_002074"
     CDS             4410..5393
                     /gene="pheS"
                     /locus_tag="RHW33_002074"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV1645574.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5408..7795
                     /gene="pheT"
                     /locus_tag="RHW33_002075"
     CDS             5408..7795
                     /gene="pheT"
                     /locus_tag="RHW33_002075"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV1645575.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQYKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTKGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     ARIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7800..8099
                     /gene="ihfA"
                     /locus_tag="RHW33_002076"
     CDS             7800..8099
                     /gene="ihfA"
                     /locus_tag="RHW33_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV1645576.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8200..9180
                     /gene="btuC"
                     /locus_tag="RHW33_002077"
     CDS             8200..9180
                     /gene="btuC"
                     /locus_tag="RHW33_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV1645577.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9243..9794
                     /gene="btuE"
                     /locus_tag="RHW33_002078"
     CDS             9243..9794
                     /gene="btuE"
                     /locus_tag="RHW33_002078"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV1645578.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9794..10543
                     /gene="btuD"
                     /locus_tag="RHW33_002079"
     CDS             9794..10543
                     /gene="btuD"
                     /locus_tag="RHW33_002079"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV1645579.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10621..11085
                     /locus_tag="RHW33_002080"
     CDS             10621..11085
                     /locus_tag="RHW33_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1645580.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11332..12045
                     /gene="ydiV"
                     /locus_tag="RHW33_002081"
     CDS             11332..12045
                     /gene="ydiV"
                     /locus_tag="RHW33_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV1645581.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12108..13544
                     /locus_tag="RHW33_002082"
     CDS             12108..13544
                     /locus_tag="RHW33_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV1645582.1"
                     /translation="MTLSFITRWRDELPATYTAPSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGPGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLNDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASRLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13548..13739)
                     /gene="hemP"
                     /locus_tag="RHW33_002083"
     CDS             complement(13548..13739)
                     /gene="hemP"
                     /locus_tag="RHW33_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV1645583.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQTIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13871..14917)
                     /gene="aroH"
                     /locus_tag="RHW33_002084"
     CDS             complement(13871..14917)
                     /gene="aroH"
                     /locus_tag="RHW33_002084"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV1645584.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(15074..15907)
                     /gene="ppsR"
                     /locus_tag="RHW33_002085"
     CDS             complement(15074..15907)
                     /gene="ppsR"
                     /locus_tag="RHW33_002085"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV1645585.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16240..18618
                     /gene="ppsA"
                     /locus_tag="RHW33_002086"
     CDS             16240..18618
                     /gene="ppsA"
                     /locus_tag="RHW33_002086"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV1645586.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLNEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            19108..20508
                     /locus_tag="RHW33_002087"
     CDS             19108..20508
                     /locus_tag="RHW33_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV1645587.1"
                     /translation="MSCYLKVLICAIVALAIWFSPVPDGLTLLTWHILAIFITTVVAF
                     ILQPLPVGAIALIAISFIMLTGMMKTSEALKGFSSTTVWLIVAAFLYAKGFIKTGLGR
                     RIAYLLIRGFGGSSLRLGYTLALSDMIIAPATPSNTARAGGILFPIVRSVSNSFGSEP
                     DQGPRKIGAYLMQTVFHSNCLSSSMFMTASAPNALIVSLAASTLFVDISWGMWTLSAL
                     VPGIIAFITMPLVIYKLYPPEIKKTPEAKALAQEELIKMGPVTRDERVTIGIFLLSLL
                     AWSTSKWTGLDATAVALSGVCLMLMTRIITWQDVQSEKGAWDILVWLGVMICMADKLN
                     QLGLFKWFAVTTSALFTGIPWEITLTVLLIVYCYSHYFFAGSTPHVVAMYAAFGSVSV
                     AAGAPPMMAALSLAFVTNLMSGISHYGNGPAVIYYGAGYVSQREWWRLGFIVMLLNIV
                     IWFGLGAVWWKILGLW"
     gene            20544..21503
                     /locus_tag="RHW33_002088"
     CDS             20544..21503
                     /locus_tag="RHW33_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDV1645588.1"
                     /translation="MNLKDIQVAIVGKLSDRLMAKVESTFTAYRLWELTDADAFYTQI
                     APDIQALVTSGNPVMGASRALIEKFPNLKIIVSNGVGYDPIDIVAAREHGVIVTNTPG
                     VLNDCVADIGMALLLNVARRINIADRYVRDGRWPNEGRFPMATKVSGKRCGIVGLGNI
                     GNAVARRAAAFEMEIHYFDPKPHNKPGWTAHDSLVSLAQAVDFLVLTLPGGASTRGVI
                     DKTVLQALGGTGHLISISRGSVVNETDLIDALQNNIIAGAALDVYANEPHVPEQLMSL
                     DNVVLTPHIASGTSETFNAMADLVFDNLQAFFSGQPVITPVTQ"
     gene            21514..21993
                     /locus_tag="RHW33_002089"
     CDS             21514..21993
                     /locus_tag="RHW33_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV1645589.1"
                     /translation="MIYGDITYLSEQQKRTFHPVIARVIEYLSQHDLNSVPVGEKVFI
                     QDDSIFYTVVEPMTKPLHECRPEVHRKYLDVHFLIAGEERICVDFDDGENSVVESHLG
                     DRDVIFYADERINDRIITLTPGQFVVVFPEEVHRACYKVDNVEKIKKTVVKIDMSLL"
     gene            22006..22749
                     /locus_tag="RHW33_002090"
     CDS             22006..22749
                     /locus_tag="RHW33_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1645590.1"
                     /translation="MTKIALVTGGSKGIGYAAARALYDIGYTVIVAARNEETLQQCAQ
                     ELAKERFIPLVADVTDESAVVALFAEIKARFGRLDLLFNNAGNNSPAKSIEEVEFTEW
                     IRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVT
                     GLTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAA
                     IQMMAQLPVTTNVLEITIMANNMPYVGRG"
     gene            complement(22800..24440)
                     /gene="fadK"
                     /locus_tag="RHW33_002091"
     CDS             complement(22800..24440)
                     /gene="fadK"
                     /locus_tag="RHW33_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV1645591.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKIFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARIVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIVQRLEQSCVEA"
     gene            complement(24497..24790)
                     /locus_tag="RHW33_002092"
     CDS             complement(24497..24790)
                     /locus_tag="RHW33_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV1645592.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(24787..26076)
                     /locus_tag="RHW33_002093"
     CDS             complement(24787..26076)
                     /locus_tag="RHW33_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1645593.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(26132..27070)
                     /locus_tag="RHW33_002094"
     CDS             complement(26132..27070)
                     /locus_tag="RHW33_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HDV1645594.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLAQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSSLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(27090..27854)
                     /locus_tag="RHW33_002095"
     CDS             complement(27090..27854)
                     /locus_tag="RHW33_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV1645595.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            28170..29081
                     /locus_tag="RHW33_002096"
     CDS             28170..29081
                     /locus_tag="RHW33_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV1645596.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWLLTNTELSQAEISWRVGYENVDHFGKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(29124..30275)
                     /locus_tag="RHW33_002097"
     CDS             complement(29124..30275)
                     /locus_tag="RHW33_002097"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV1645597.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPEPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(30289..31884)
                     /locus_tag="RHW33_002098"
     CDS             complement(30289..31884)
                     /locus_tag="RHW33_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase subunit
                     alpha/beta"
                     /protein_id="HDV1645598.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQHGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(32028..32786)
                     /gene="aroD"
                     /locus_tag="RHW33_002099"
     CDS             complement(32028..32786)
                     /gene="aroD"
                     /locus_tag="RHW33_002099"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV1645599.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(32817..33683)
                     /gene="ydiB"
                     /locus_tag="RHW33_002100"
     CDS             complement(32817..33683)
                     /gene="ydiB"
                     /locus_tag="RHW33_002100"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV1645600.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(33695..34960)
                     /locus_tag="RHW33_002101"
     CDS             complement(33695..34960)
                     /locus_tag="RHW33_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645601.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(35187..36401)
                     /locus_tag="RHW33_002102"
     CDS             complement(35187..36401)
                     /locus_tag="RHW33_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645602.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRSPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRNKTR
                     HHSLKENVAPGG"
     gene            complement(36501..36857)
                     /locus_tag="RHW33_002103"
     CDS             complement(36501..36857)
                     /locus_tag="RHW33_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV1645603.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
BASE COUNT         9352 a         8771 c         9383 g         9370 t
ORIGIN      
        1 tggatgggtg aagtggcaaa gttaatggta aaaagggcag tggaataaca attttgtgat
       61 atttttcgac agttagcggt aaattcctga gtaacacagt atgacagcat cgaacttaag
      121 tcagaggtat caagtagggt gatgagttgg cgcgcaaatt gattaatagg ctctgatgaa
      181 ggcgttggta tgtcgctatc gatgtgcatt ctatgaggag acttataatg agttctgata
      241 ataatgtcgt tttgactcat aatctattcg ccttatatat gtctggaggt tttgaggatg
      301 atgattaatt attatcgcca gaacgaaatg tgcacgaaaa attgctataa gaattgtgtc
      361 acttttatca ttgtgatatg accacttcgt cattaatctc atgatcgttt aaatactacc
      421 gccaaatcat gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag
      481 taaacctttc agacgctccg tgatgttatc agttgttctt taagcgtttt gctggtgtac
      541 tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt
      601 tttagtgtta acatcgctca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt
      661 acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat
      721 aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata
      781 aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgatcac gctgtaagcc
      841 ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg
      901 ttaatggcga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca
      961 ttaccgccaa agacgaagaa ggtctggaga tcattcgtca ctcctgtgcg cacctgttag
     1021 ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca
     1081 acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac
     1141 tcgagaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca
     1201 gctggcacga agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtctccattc
     1261 ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg
     1321 atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga
     1381 aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg
     1441 gtacggcgtg ggcagacaaa aaagcactta acgcttacct gcagcgcctg gaagaagccg
     1501 cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag
     1561 cgccgggtat ggtattctgg cacaacgacg gctggaccat cttccgtgaa ctggaagtgt
     1621 ttgttcgttc taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg
     1681 accgtgtcct gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca
     1741 cgtcttctga gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa
     1801 ttttcaacca ggggctgaag tcttatcgcg atctgccgct gcgtatggcc gagtttggta
     1861 gctgccaccg taacgagccg tcaggttcgc tgcatggcct gatgcgcgtg cgtggtttta
     1921 cccaggatga cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat
     1981 gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac
     2041 tctccactcg tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg
     2101 acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg
     2161 ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt
     2221 gcggtacagt acagctggac ttctccttgc cgtctcgtct gagcgcctcc tatgtgggtg
     2281 aagacaacga gcgtaaggta ccggtaatga ttcaccgcgc aattcttggg tcgatggaac
     2341 gtttcatcgg tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccgg
     2401 ttcaggttgt tatcatgaat attaccgatt cacagtctga ttacgttaac gaattgacgc
     2461 aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct
     2521 ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag
     2581 aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg
     2641 acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat
     2701 tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca
     2761 atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta
     2821 ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga
     2881 tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg
     2941 aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa
     3001 ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc
     3061 gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg
     3121 cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg
     3181 cagtggtcga atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc
     3241 ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagccttcg ggcttcacag
     3301 gctttgttcg cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa
     3361 aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta
     3421 agcacaagca cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc
     3481 acctgcgtcc gaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc
     3541 cgtacgcata agccgttaac gtttttaact ttttaattag aatatagata caggagagca
     3601 catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa
     3661 caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt
     3721 atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa
     3781 ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc
     3841 atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca
     3901 gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa
     3961 gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac
     4021 ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag
     4081 caatgaatgc tgctattttc cgcttctttt tttactttag cacctgaatc caggaggcta
     4141 gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg
     4201 tatgcgcgtt ttgaaaaata aaaagccagt ctatcaggcg taattgtcgc agccagtctg
     4261 gacacggcca gtgcggagaa accggagcgt acttaagtac gtgaggattt cgagcactgc
     4321 ccgggaccaa aatggcaagt aaaatagcct gatgggatag gctctaagtc caacgaacca
     4381 gtgtcaccac tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg
     4441 cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat
     4501 atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag
     4561 aagagcgtcc tgcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc
     4621 tgaatgcgcg taaagcggaa ctggaaagcg ctgcactgaa tgcgcgtctg gcggcggaaa
     4681 cgattgatgt ctctctgcca ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc
     4741 gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc
     4801 cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg
     4861 cgcgcgctga ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct
     4921 ctggcgtaca gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg
     4981 gccgtgttta tcgtaacgac tacgaccaga ctcacacgcc gatgtttcat cagatggaag
     5041 gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc
     5101 tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg tccttcctac ttcccgttta
     5161 ccgaaccttc tgcagaagtg gatgtcatgg gtaaaaacgg taaatggctg gaagtgctgg
     5221 gctgcgggat ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact
     5281 ctggtttcgc cttcgggatg gggatggagc gtctgactat gttgcgttac ggcgtcaccg
     5341 acctgcgttc attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga
     5401 atagattatg aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag
     5461 cgatgcgctg gcaaatcaaa tcactatggc gggcctggaa gttgacggtg tagagccggt
     5521 tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa
     5581 cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt
     5641 ctgtggtgcg ccaaactgcc gtcagggcct gcgtgtggcg gtagcgacca ttggtgctgt
     5701 tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat
     5761 gctgtgctcc ttctctgaac tgggaatttc tgacgatcac agcggcatta tcgaactgcc
     5821 tgcagatgcg ccgattggca ccgatatccg cgaatacctg aaactcgatg acaacaccat
     5881 cgaaatcagc gtaacgccaa accgtgccga ctgtttaggt atcattggtg ttgcgcgtga
     5941 cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac
     6001 catcgacgac acgctgccga ttacagtcga agcgccggaa gcctgcccgc gttatcttgg
     6061 ccgtgtggta aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact
     6121 gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtgctgct
     6181 cgaactgggc cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt
     6241 gcggatggcg aaagagggtg aaacgctggt actgctcgac ggcactgaag cgaagctgaa
     6301 tgctgacacc ctggttatcg ccgaccacaa caaggcgctg gcgatgggcg gcatttttgg
     6361 tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc ctgctggaat gcgctttctt
     6421 cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca
     6481 ccgttatgag cgtggcgttg atccggcact gcagtacaaa gcgatggaac gtgcgacccg
     6541 tctgctgatc gacatctgcg gtggtgaggc tggtccggta attgatatca ccagcgaagc
     6601 aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg
     6661 ccatcatatt gcggatgagc aggtaactga cattctgcgt cgtctcggct gcgaagtgac
     6721 caaaggcaaa gacgagtggc aggcagttgc gccgagctgg cgtttcgaca tggagattga
     6781 agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc
     6841 ggtacaggca agcctgatta tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt
     6901 gaaaacgctg ctcaatgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc
     6961 gaaagtgcag cagatgatcc atccaggcgt tgaagcgtta ctgctgccaa gcccgatctc
     7021 tgttgaaatg tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta
     7081 caaccagaac cgtcagcaga accgtgcgcg cattttcgaa agcggtctgc gtttcgtacc
     7141 agatactcag gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg
     7201 taaccgttac gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa
     7261 aggcgatctt gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc
     7321 agaagcgaat ccggcactgc atccggggca atccgcagcg atttatctga aaggtgaacg
     7381 tattggtttt gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg
     7441 cactctggtg ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg
     7501 cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt
     7561 tcccgcagcg gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt
     7621 aaacttattt gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat
     7681 aagcctgatc ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt
     7741 cgccaaatgt gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta
     7801 tggcgcttac aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc
     7861 gggatgccaa agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg
     7921 gcgaacaggt gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc
     7981 cgggacgtaa cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct
     8041 tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat
     8101 ctgatctaac taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc
     8161 gtaaaatcaa cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa
     8221 caacagcgac aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt
     8281 ctcttaagcc tttgcgccgg tgaacaatgg atttcgccag gtgactggtt ttctcctcgt
     8341 ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt
     8401 gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa
     8461 cctggactac ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt
     8521 gggcaagggc aactccccaa ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc
     8581 atcactttaa tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg
     8641 gctggcgttg cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc
     8701 acctctgttg atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac
     8761 tggcggcaaa gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag
     8821 tccaggccga tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc
     8881 ctgtggttct ggcgcaatgt gctggtggca gcgaccggct ggatggttgg cgtcagtgta
     8941 gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc cacatattct tcggttgtgt
     9001 ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg
     9061 ctgctggccg atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg
     9121 gtcaccgcaa cgttgggtgc gccggtgttt atctggttat tgttaaaagc aggacgttag
     9181 ccgcaaaaag acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa
     9241 cgatgcaaga ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc
     9301 tggagaagta cgccggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa
     9361 cgccgcaata tgagcagttg gagaatattc agaaagcctg ggccgatcga ggttttgtgg
     9421 tgctgggatt cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta
     9481 aaacttactg taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta
     9541 atggcgaagg acgccatcca ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg
     9601 cgccggaaga gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc
     9661 ctgatgatat tttatggaat tttgagaaat ttttagttgg cagggacgga ttggtcatcc
     9721 agcgtttttc cccggatatg acgccggaag accccatcgt gatggaaagc attaaactgg
     9781 cgttggcaaa ataatgtcta ttgtgatgca gttacaggat gttgcggaat caacccgtct
     9841 ggggccgctt tctggcgagg ttcgggctgg ggagatcctg catctggtgg ggccgaatgg
     9901 cgcgggtaag agtaccttac tggcacgaat ggccggaatg accagcggta agggaagcat
     9961 tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc
    10021 ctatctttca caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact
    10081 gcatcagcac gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct
    10141 tgatgacaaa ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg
    10201 tcttgctgcg gtggtgctgc aaatcacacc acaagccaat cccgcaggcc aattgctgct
    10261 tcttgatgag ccgatgaaca gtcttgatgt tgctcaacaa agtgcgttag acaaaattct
    10321 gagcgcgctg tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac
    10381 attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg
    10441 cagagaagag gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg
    10501 tctggatatc gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga
    10561 aaatcgggta attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga
    10621 atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccatcataaa
    10681 gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac
    10741 cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg
    10801 gactgttcgg gatttgtggt tgtgacgatg cgcgatcgtt tcgatttgca gctgccccga
    10861 gaaaccaaag aacaagcctc tatcggcacg caaattgata aagacgagtt gctgcctggt
    10921 gacctggtct ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat
    10981 accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat
    11041 aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaattgg
    11101 acggcgatga tacatcgccg tccatttact ccagaataca gtagtatttt gcagacaaaa
    11161 attccgtaaa tggattattc gtttataatc aggataatct aaaaaaagga cccctgaaaa
    11221 agtggttaac atggaacaaa tgaaagttac tccttatgta attgttatgt cttatggcct
    11281 catccctttt agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt
    11341 tttttggaga atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa
    11401 gaccttgtcg gcgttgagtt aattacccat ttctccagtg aagatggcac ggtgcgtatc
    11461 ccgacgagtc gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag
    11521 cagttggaat tactaaaatc atgccaacac ttttttattc aacataaact ctttgcctgg
    11581 cttaatttaa cgccacaagt ggctacatta ttattagaca gggataatta cgcaggcgaa
    11641 ctattaaaat atccgtttat tgaactgttg attaatgaga attacccgca tctcaatgaa
    11701 ggaaaagata atcgcggtct tttatcctta tcgcaagtgt atccccttgt gttaggtaat
    11761 ttgggagcag gcaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg
    11821 ctggataaaa gttttattca gcaacaaatt acgcaccgct ccttcgaacc gtttattcgt
    11881 gcgatccagg cgcaaatttc cccttgttgc aattgcatca tcgcgggggg cattgatacg
    11941 ccagaaatat tggcacagat tacccccttc gatttccatg ctctccaggg atgcctgtgg
    12001 cctgccgttc ccataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg
    12061 gtgtttaaga cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt
    12121 ttattacccg ctggcgcgat gaattgccag caacctatac agcaccttcc cctacgcctt
    12181 taaataatgc ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat
    12241 cgtcgctgtt taaaaatggc ccaggtgtct ggggcggcga aaccctactg cctggtatgt
    12301 caccactggc gcaggtttac agtggtcatc agttcggcgt ctgggcgggg caactgggtg
    12361 atgggcgcgg cattttactt ggcgagcaac tgcttgctga tggcactaca atggactggc
    12421 atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac
    12481 gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga
    12541 cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagccag
    12601 gcgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt
    12661 actatcgccg cgagccggaa aaggttcgtc agttggctga ctttgccatt cgtcattact
    12721 ggtcacatct tgaagatgat gaggacaaat accgtctctg gtttaacgat gttgtcgcac
    12781 gtaccgcatc gttaattgcc caatggcaga cggtcggctt tgcacatggg gtgatgaata
    12841 ccgacaacat gtcgctgctg ggactgacgc ttgattacgg gccgtttggt tttcttaatg
    12901 attacgaacc cggttttatt tgtaatcact cggatcatca agggcgttac agctttgaca
    12961 atcaacctgc tgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg
    13021 ttgccgtaga tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt
    13081 atggacaacg gatgcggcag aaactgggct tcatcacgga gcaaaaagag gataacgcgc
    13141 tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacatttc
    13201 gcatgctgag tctgaccgag cagcacagcg cggcgtcacg gctacgtgat gagtttattg
    13261 atcgtgcggc atttgatgac tggtttgccc gttatcgagg gcgtttgcaa caagacgagg
    13321 ttagcgatag tgagcgtcag caactgatgc aaagcgttaa cccagctctg gtattgcgca
    13381 actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgact gaattgcacc
    13441 gcctgcatga ggcgttgcga aatcctttca gcgacagaga tgatgactat gtcagtcgtc
    13501 cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac
    13561 agcagcttgc cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc
    13621 agtttgccat ccggacctaa cagtgtctgg ctggaaattc gccgaatagt ctgcggggaa
    13681 gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata
    13741 aaaattaata ataattgata taacaatgat tcttattatc aatattatgg ctgaccagag
    13801 caagcagtaa tgtgttgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat
    13861 gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg
    13921 tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgactgc
    13981 cgacgatttt ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg
    14041 tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct
    14101 gcttttggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt
    14161 gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac
    14221 gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt
    14281 tgtctggcga gaggaacata tggctggcgc gggctgcgcg gatagcatcc acagcaatcc
    14341 gcgtattgcc atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt
    14401 cacggtggat ctgactttcg gtagtacgtg cgccaatcgc gccccaactg attaaatcag
    14461 caataaactg accggtcacc atatcgagga actcagtcgc ggttgggacg cccagctcat
    14521 ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt
    14581 ttaaatctgg atcggagatt agtcctttcc agccgacaac agttcgtggt ttttcaaaat
    14641 aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg
    14701 tggcgtactc cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca
    14761 gtcgcttatc ttcaccattg agtatttttt caattctgcg gcgggagtcg gtgacatggg
    14821 tggcgacgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct
    14881 caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc
    14941 ttctacggca ggactgccgg gaagtgatgc gcatcacgat aaaccaatcg ctgctgattt
    15001 caagtgcgag gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaataagcta
    15061 atgcactagt tctctagtac attcggcgac taaggcccat gatatcgagg atcttggtgg
    15121 caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca
    15181 aggcttccac ttccgcgact tccatcctgc actgacgaag cgaggcatag cgactgttct
    15241 ccctacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt
    15301 tatgctgaag cggtttgagc gacgcaggta gcaccagatt atccatatcg tcggcaataa
    15361 aggggtagtt tgccgcgcgg ataccaaatt gcattgccag atacagactg gtgggggttt
    15421 taccacagcg agaaacaccg agcaggatca cctgagcctg atccagattg cgcaacgaaa
    15481 tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga
    15541 gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct
    15601 cttgttgtag cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca
    15661 agatgatggc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacgcctg
    15721 tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga
    15781 caaacggcag cgtgatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta
    15841 atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat
    15901 tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg
    15961 cacagaagcg tagaacgtta tgtctggttt ataaaatgaa ccttcaattt tattttttat
    16021 gaaaacagca tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg
    16081 aagagagcag atttgcgcaa cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct
    16141 tgaacgattc accgtttttt tcatccggtt aaatatgcaa agataaatgc gcagaaatgt
    16201 gtttctcaaa ccgttcattt atcacaaaag gattgttcga tgtccaacaa tggctcgtca
    16261 ccgctggtgc tttggtataa ccaactcggc atgaatgatg tagacagggt tgggggcaaa
    16321 aatgcctccc tgggtgaaat gattactaac ctttccggaa tgggtgtttc cgttccgaat
    16381 ggtttcgcca caaccgccga cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag
    16441 cgcatttatg aactgctgga taaaacggat attgacgatg ttactcagct tgcgaaagcg
    16501 ggcgcgcaaa tccgccagtg gattatcgac actcccttcc agcctgagct ggaaaacgcc
    16561 atccgcgaag cctatgcaca gctttccgcc gatgacgaaa acgcctcttt tgcggtgcgc
    16621 tcctccgcca ccgcagaaga tatgccggac gcttcttttg ccggtcagca ggaaaccttc
    16681 ctcaacgttc agggttttga cgccgttctc gtggcagtga aacatgtatt tgcttctctg
    16741 tttaacgatc gcgccatctc ttatcgtgtg caccagggtt acgatcaccg tggtgtggcg
    16801 ctctccgccg gtgttcaacg gatggtgcgc tctgacctcg catcttctgg cgtgatgttc
    16861 tccattgata ccgaatccgg ctttgaccag gtggtgttta tcacttccgc atggggcctt
    16921 ggtgagatgg tcgtgcaggg tgcggttaac ccggatgagt tttacgtgca taaaccgaca
    16981 ctggcggcga atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa aatccgcatg
    17041 gtttacgcgc cgacccagga gcacggcaag caggttaaaa tcgaagacgt accgcaggaa
    17101 cagcgtgaca tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa acaggccgta
    17161 caaattgaga aacactacgg tcgcccaatg gatattgagt gggcgaaaga tggccacacc
    17221 ggtaaactgt tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg
    17281 gagcgttata cgctgcattc acagggtaag attatcgccg aaggccgtgc tatcggtcat
    17341 cgcatcggtg cgggtccggt gaaagtcatc catgatatca gcgaaatgaa ccgcatcgaa
    17401 cctggtgacg tgctggtcac tgacatgacc gacccggatt gggaaccgat catgaagaaa
    17461 gcatctgcca tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt
    17521 gaactgggca ttccggcggt agtgggctgt ggtgatgcaa cagaacggat gaaagacggt
    17581 gagaacgtca ctgtttcttg tgccgaaggt gataccggtt acgtctatgc ggagttgctg
    17641 gaatttagcg tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg
    17701 atgaacgtcg gtaacccgga ccgagctttc gacttcgcct gtctgccgaa cgaaggcgtg
    17761 ggacttgcgc gtctggaatt tatcatcaac cgtatgattg gcgtccaccc acgcgcactg
    17821 cttgagtttg acgatcagga accgcagttg caaaacgaaa tccgcgagat gatgaaaggt
    17881 tttgattctc cgcgtgaatt ttacgttggt cgtctgaatg aagggatcgc gacgctgggt
    17941 gccgcgtttt atccgaagcg cgtcattgtc cgtctctctg attttaaatc gaacgaatat
    18001 gccaacctgg tcggtggtga gcgttacgag ccagatgaag agaacccgat gctcggcttc
    18061 cgtggcgcgg gacgctatgt ttccgacagc ttccgcgact gtttcgcgct ggagtgcgaa
    18121 gcagtgaaac gtgtgcgcaa cgacatgggg ctgaccaacg ttgagatcat gatcccgttc
    18181 gtgcgtaccg tagatcaggc gaaagcggtg gttgaggaac tggcgcgtca ggggctgaag
    18241 cgtggtgaga acgggctgaa aatcatcatg atgtgtgaaa tcccgtccaa cgccttgctg
    18301 gccgagcagt tcctcgaata tttcgacggc ttctcaattg gctcaaacga tatgacgcag
    18361 ctggcgctcg gtctggatcg tgactccggc gtggtgtctg aattgttcga tgagcgcaac
    18421 gatgcggtga aagcattgct gtcgatggct atccgtgccg cgaagaaaca gggcaaatat
    18481 gtcgggattt gcggtcaggg tccgtccgac cacgaagact ttgccgcatg gttgatggaa
    18541 gaggggatcg atagcctgtc tctgaatccg gacaccgtgg tgcaaacctg gttaagcctg
    18601 gctgaactga agaaataaaa taaatacccg gcggcgtttt gtcgccgggt ccatttctat
    18661 atccccacct tttaacaacc tacttcaggc aaacgcgctc gtgcaatata aaaagcttcc
    18721 gagcatcatc tgaataattg gctggtgttt tttctgattt taattcgttt gagatcttct
    18781 ccacagaata attctcgata ccattttttc tcaaagtcga atgtgaccac tcgtcaattt
    18841 ccttaagcat atgcgtaatt ttttcgtcca gagttcgttg gattgcacga agtcactggc
    18901 gagtcccgtt tttatactgt cttcaaccac agcataaaaa gagatatggc tatgaaaaat
    18961 taccgcaccg ttattcagac ataagcgcag attatgtaat tcagagaatt tattcgaact
    19021 aaagccgatt ttctgatttg tctggctgta cagaaaacag gataaattcc ctccacgagc
    19081 ggagggaaac agctacaggg attgcatatg agttgctatt tgaaagtcct gatttgtgcg
    19141 attgtcgcac tcgctatatg gttttccccc gtccctgacg gtttaacact cttaacctgg
    19201 cacattctgg caatattcat caccactgtg gtggcgttta ttctgcaacc gttgccggta
    19261 ggcgctatcg cgttaatagc catcagtttt attatgctta ccggcatgat gaaaaccagc
    19321 gaagccttaa aaggtttcag cagcaccacg gtatggctta ttgtcgccgc attcctgtac
    19381 gcgaaaggat tcatcaaaac ggggttagga cgacgtatcg cctatttact gattcgcgga
    19441 tttggcggca gctcgttgcg tctgggttat accttagcgc taagtgatat gattatcgcg
    19501 cccgcaacgc catcaaacac agcgcgagca ggggggattt tgttcccgat tgttcgcagt
    19561 gtctctaaca gctttggatc tgaaccggat caagggccgc gtaaaatcgg cgcttatctg
    19621 atgcagacgg tgttccattc caactgtctc tcatcctcga tgtttatgac ggccagcgcg
    19681 ccaaatgcgc taattgtatc tctggcggcc agcaccttat ttgttgatat ctcatggggg
    19741 atgtggacgc taagtgcact ggtaccgggc attatcgctt ttatcactat gccgttggtc
    19801 atttacaaac tttacccgcc agagatcaaa aagacgccag aagccaaagc gttggcacag
    19861 gaagaactta tcaaaatggg gccggtaacc cgtgatgagc gcgtgacgat tggcattttc
    19921 ctgctttcat tattggcctg gagtacatcg aaatggactg gcctggatgc gacggctgtc
    19981 gcgctttccg gcgtctgttt aatgttgatg acccggatta tcacctggca ggatgtgcag
    20041 tcagagaagg gggcctggga cattctggtc tggctgggag tcatgatctg tatggcagat
    20101 aagctcaatc agcttggcct gttcaaatgg tttgccgtca caacctcggc gctgttcacc
    20161 ggcattccct gggaaataac actgacggtt ttgctgatag tttactgcta ttcccactat
    20221 ttctttgccg gaagtacgcc gcatgtagtg gcgatgtatg ccgcttttgg cagcgtctcg
    20281 gttgctgcag gcgcaccgcc gatgatggca gctctgtcac tggcgttcgt cactaacctg
    20341 atgagcggca tttcgcatta tggcaacgga cccgcagtga tctattacgg cgcaggctat
    20401 gtctcgcaac gagaatggtg gcggctgggc tttatcgtca tgttgttgaa cattgtcatc
    20461 tggttcggac tgggggcggt gtggtggaaa attcttggcc tgtggtaagt ggtctgcggc
    20521 aaacatcaat caaggaatag tgtatgaact taaaagatat tcaggtggcg atcgtcggca
    20581 aattaagcga ccgcttaatg gctaaagttg aatctacctt caccgcatat cgcctgtggg
    20641 aactgaccga cgcagacgct ttttacacgc agatcgcgcc ggatattcag gcgctggtga
    20701 ccagtggtaa tccggtaatg ggagcttctc gggcgctcat tgaaaaattt ccgaatctga
    20761 agattattgt cagcaacggt gtgggctatg accctattga tatcgtggca gcaagggagc
    20821 atggcgttat cgtcaccaac acccctggcg tgcttaatga ttgtgttgca gacattggca
    20881 tggcgttgtt actcaatgtt gcccgacgta ttaacattgc cgatcgttat gttcgtgacg
    20941 gccgttggcc aaacgaaggg cgtttcccga tggcgacgaa agtgagtggt aagcgttgcg
    21001 gaattgttgg tctggggaac attggtaacg ctgtagcccg ccgtgcagca gcatttgaaa
    21061 tggaaattca ctatttcgac ccaaaaccgc ataacaaacc tggatggacg gcgcatgatt
    21121 cgctggtttc tctggcgcaa gcggtggatt tcctcgttct gacgctgcca ggcggggcaa
    21181 gtacacgcgg cgttattgac aaaactgtgc tgcaggctct gggggggacc gggcatttga
    21241 tcagtatttc ccggggttca gtggtgaacg aaactgatct gattgatgct ctgcaaaata
    21301 acataattgc tggtgcggca ctggacgttt atgccaacga accacatgtg ccagaacaat
    21361 taatgtcgct ggataacgtt gtccttacgc cacatatcgc cagtggtacg agcgaaacgt
    21421 ttaatgcgat ggcggatttg gtttttgata acttacaggc tttctttagc ggacagccag
    21481 ttattacccc tgttactcaa taaggcggaa aatatgattt atggtgatat cacttatctt
    21541 agtgaacaac aaaaacgaac atttcatcct gtcatcgctc gtgtcattga gtatctttca
    21601 caacatgatc tgaatagtgt tcctgtaggg gagaaggttt ttattcagga tgactcaatt
    21661 ttttataccg tggttgaacc gatgactaaa ccactacatg aatgccgacc agaagttcat
    21721 cgtaaatatc ttgatgtcca tttcttaatt gccggagaag agcggatttg tgtggatttt
    21781 gatgatggag aaaactcggt tgtagaaagc cacctgggcg accgtgacgt tattttttat
    21841 gctgatgaac gtattaatga tcggataatc acgttgacac caggacagtt tgtggtggtc
    21901 ttcccggaag aggtacatcg ggcatgttat aaagtcgata acgtggagaa aatcaaaaaa
    21961 acagtcgtga aaatcgacat gagtctatta taggtggagc tggttatgac aaaaattgcc
    22021 ctggtgacag ggggaagtaa aggtatcggc tacgcggcag caagggcgct ctatgacatt
    22081 ggatatactg ttattgttgc cgcgcgtaac gaagaaacct tacagcaatg tgcacaagaa
    22141 ttagccaagg aacgctttat tcctctggtt gctgatgtaa cggatgagtc cgctgtcgtt
    22201 gcactatttg ccgagattaa ggctcgtttt ggtcgactgg atctcttgtt caataatgcg
    22261 ggcaataata gtccggcaaa aagtattgaa gaggttgagt ttactgagtg gatacgcgta
    22321 tttaacgcca atattcactc gacatttttg tgttcgaaag aggcgattaa aattatgaaa
    22381 gcgcagcagc caatgggagg acgtattatt aacaatgcgt cgatttccgc actgacgccg
    22441 cgcctctata ctgcgccata cacggcgtcg aaacatgctg ttaccgggtt gaccaaggcc
    22501 atttccctgg attgtcgcaa atttaatatc gcttgcgggc aaattaatat cggcaatgcg
    22561 gaaacgacat tatcgaatcg gatgcgtaag ggcgtttatc aggcagatgg cgctattaaa
    22621 gcggaagata tgatgaatgt tgctgatgtt gcagcagcca ttcagatgat ggcccaattg
    22681 ccagtaacca cgaatgtgct ggaaataacg attatggcaa ataatatgcc atatgtcgga
    22741 cgtggttaat cgggattgaa taaagcacct tgcgtataag gtgctttatt atttatgact
    22801 tatgcctcaa cgcatgattg ttccagccgt tgaacaatat ctttccgtaa caaaaacttt
    22861 tgtattttac ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat
    22921 ttatattttg cgacccgttt acggctaaaa aaagccacca cctcttccag cgataatgaa
    22981 tgatgcggcg ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc
    23041 attgcaacca cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca
    23101 cggctgctaa tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt
    23161 atatagccag cttcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc
    23221 agcgcacggg cggttaattc aggttcatca aaatatccca taaacacatt ggggccacgc
    23281 gaggcttctt caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta
    23341 atctctacac ctgcggcagc gtaaccatcg gtgtgcataa agcgcgacaa agggtcgtcg
    23401 agattcacca ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg
    23461 ccgcgctgct ggcattcacg cgccactttt ttgggaattg tggtaccgcc gcaaagaaag
    23521 aaacgcagcg ctgaaaggtc tgcgggctgt ttctctagta aattcaaaag atcatagaca
    23581 aacggcgttg cgccgagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca
    23641 tcaggagtga aaatatctaa caacacgatg cgagcaccaa tcaaaaatgg tgctgttacg
    23701 ccatgcagaa agcccgttgc gtggccgagt ggcgcaggca tcataaagac atcctgccag
    23761 gtcagattca gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc
    23821 atcacgccct ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg
    23881 ccgtgggtcg ttatcgccgt ggtcagtggg gtattgtcgg cgataatctg acttaatgag
    23941 agggaagagg tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc
    24001 tgattttgaa gcggcaggat taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca
    24061 aagaatattt ttgcctgaca cttattaagc acccagacca gttcagcttc tcgccaggaa
    24121 ggcaacaacg gtacggaaac cgcaccggtt ttcaggcagg caagatagat aacagtaaat
    24181 tcacaccagc caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac
    24241 atccagtttg ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgctcca
    24301 tgattatcga ccacggcaat tttgtcaggc atcgcacgag cggtctgctg ccagtaatca
    24361 gccagcgaag catcgcccca taacccttgc tgacgatacg ccgcacgacg ttgttcgtta
    24421 aacgttaatg tcactttcat tttggactct cgaaacagaa catcaggccc gagatgcggg
    24481 cctgtgggat gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt
    24541 gccactgttc gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac
    24601 cggcggaatc aaaatgaatg ttgccggcat catcctgctt ataaagtccg gcagggcagg
    24661 ctttcattaa tttacggaat tcattgatat cgggatttgc cgccaaaatg atatgcgggt
    24721 ggccctcatc aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt
    24781 tctggctcat agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat
    24841 tttcttcgcg tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt
    24901 gaacatatcg ttcatgatgt cggcgaccat gcgcggatac tgactaaaca ggcgcggatt
    24961 ttccatcagc gccgggatct tgcgaaaatg ctgcatatcg cgcataacac aactttgttc
    25021 cagctcacgt ttgtattgcg caagactgct ggcggagaaa tccgcgcgtt ctttggcggc
    25081 gatcaccgtt gtggcggcag cctgagccga tgcaatggct aaatccatgc cgcggaccgt
    25141 aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc cctcgttaac
    25201 cagttgcggc accattgcca gaccgccttc cggcaccata tgcgcggaat attcaagcaa
    25261 cttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg
    25321 cggcacgctt ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaacgatat
    25381 ggaatcctta ttggtataga ggaatccgcc acccatcagg ccgtcagaag gggaaccggc
    25441 aaacagccag gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg
    25501 tgtgaggcca ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac
    25561 cattcccagc gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc
    25621 ttcgagaata tcatccccag cctggacgcc agtgaccttg tttccttcac gaaccagcgc
    25681 atcgacgcga actcccggga taaactgtgc gcccgcctgc tcggcttgtt ccatcaacca
    25741 cgggtccaga cgattacgca atacggtata agatgcgtgt tgcggaacat ctggttgctc
    25801 gcggtgaaaa tcgagggtaa cggcgctctc ttcggttaag aaggagattt tctcgcgtgt
    25861 gaccttgcgt tctaccggcg ctgatgctgc aaagcctgga atgattgctt caagtgtgtg
    25921 ggcataaaga cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac
    25981 caggacatcc agcccggctc gtgccatgac cagtgcggca acgctaccag caacgcccgc
    26041 accgaccaca atggcatcaa atttgtcatc cgacatgcgt tatgctcctt tcccgctgcg
    26101 tttacagcgg gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag
    26161 ggacgacttt gtagatatcg cccaccagac cgtagtcggc atagttgaag attggcgcat
    26221 ttttatcttt attgatggcg acaatcactt ttgcgccgtt gccgccaacc atatgttgga
    26281 tctgcccgga gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac
    26341 cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga
    26401 cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca
    26461 gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt
    26521 tcgcacggcg ttcgcgacag agaatttcat gacgcggagc aacgtaaggt accgtttctg
    26581 tcgggcactg atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa
    26641 tgatcgccag cgagctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt
    26701 gttcggcgca aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc accagcgccg
    26761 cattcagttg cacacttaac cgtgctgcca gagcttttcc acgtttcgtc gcggccagta
    26821 gcaacatagc ggggtgttta tctttcagca gagcagcaat gctttcggcg taattttcag
    26881 tgcgttgcag cgcgtcgttt tgcgcaagaa cataaataca ttttggacca taaggcataa
    26941 ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag
    27001 cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta
    27061 attgactcat cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc
    27121 agctcggcaa tggcctccgg cgaatcgtta tcgagaatga tgtgcttacg ttctgtttgc
    27181 ggcggtacgc gaatgccggt cagttcagca agtggtgcgc tctggctcca gccaatatca
    27241 cttgcctgcc actgattcac cggtttttta cccgcgccaa gaatggcttt catcgaagga
    27301 atgcgtggca cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata
    27361 acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata
    27421 gcactcaccg cattaatcac cggaagttgc agaatttctc cgaccagcaa gccaacctgc
    27481 tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca
    27541 actgctgccg ccagcgcttt tgcggtatcg agaggcagtg catgctcaag ttgtgcatcc
    27601 tgcaccatat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc
    27661 tgcaacaatg agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcaagctgg
    27721 cttgcagctt caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa
    27781 ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag
    27841 gttattattt tcatgatttc tcctgtgagt cgcatacgga accatctccg atggaaggta
    27901 aaaatggcct gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag caagctgttt
    27961 tccttgccaa caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc aatttgcggc
    28021 aatatagtta ttccatgacc gtacaatctt gcattcagtg taaataatct tgtttagctc
    28081 tcgctaaacg cggttgtata accgcaatac acgccgtatt ttatccggca tatattgttt
    28141 ttgcttgctc atggaatagg gaggttgtca tgtatcaacg ctgttttgat aatgccagtg
    28201 aaacgctgtt tgtcgccggt aaaacgccac ggctttcacg ttttgcattt agcgatgatc
    28261 caaaatggga gtctggacat cacgttcatg acaatgaaac cgagctgatt tacgtcaaga
    28321 aaggggtggc aaggtttacc atcgattctt cgttatatgt cgcgcatgca gatgatatag
    28381 tggtgataga acgcggcagg ctgcatgcgg tggcctctga cgttaacgat ccggcaacga
    28441 cgtgtacctg tgcgctgtac ggctttcagt ttcagggggc tgaggaaaat cagctactgc
    28501 aaccgcattc ttgtccggta attgccgcgg ggcagggaaa agaagtcatt aaaaccttat
    28561 ttaatgagct aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct tcgttatggg
    28621 atgcatttgc ctatacatta gcaattcttt actacgaaaa ctttaaaaat gcttatcgtt
    28681 cggagcaggg gtatattaaa aaagatgttc tgataaaaga tattcttttc tatctgaata
    28741 ataattatcg cgaaaaaatc actttagaac agttatcgaa aaaatttcgt gccagcgtca
    28801 gttatatttg ccatgaattt accaaagagt atcgtatttc cccaattaac tatgttattc
    28861 aacggcgtat gacggaagcg aaatggttac tcactaatac tgaattatca caggcagaga
    28921 tctcctggcg tgtgggttat gaaaatgtcg atcactttgg caaactgttt ttgcgccatg
    28981 tcggctgttc acctagcgat taccgcaggc aatttaaaaa ctgttttgcg gaacaagaaa
    29041 tcctatctga atttcctcaa ccggtaagtc ttgttggata aatctggcgg cgcagtttgt
    29101 taagaagctg cgccgcctgc agattatttg ttctgatagt ctttcaggat ctgccgacct
    29161 gctacgtaaa tcataatttc gtctgtaccg ccgccgatac gttcacaacg gacatcacgc
    29221 cagaagcggg agacgcgcgc ttcatcggta tagcccagac cgcccatgat ttgaatcgca
    29281 tcatcaatga cttccattgc ggtacgtgcg caatacagtt ttgccagcgc cgcgctggtg
    29341 cgcagtgact gatgctgatc ggcttgccat gccactttca gcaccatgtt gcgcatgttg
    29401 tcgatcttga tcgccatcag cgccagtttt tcctggatca tctggttatg accaatgggc
    29461 ttaccaaaag cgatacgttg gttggcatag cgggcggcat cttcaaaggc gcattcggca
    29521 aagccggtgc tgcgcgcggc gttgatcagg cgctccatct caaagttgta catcacattg
    29581 aggaaaccca ttccttcttc gcccaccatg tcgctctctt caacttccac gttgtcgaga
    29641 tagacttcgc aggtgctgag catatgccag ccgattttat gtagcgggtt tatcttaatg
    29701 ccgggcttac tggagtcgac ccaccacagg gtgaaggctt ttttgggatc tttcggttca
    29761 ggatcgcgcg ccagcaccag catatacggg tactctttcg caccggtaat aaaggttttc
    29821 tgtccgttga tgtaaacctt gccgttttta cgcgtgtaag tggtagtggc actgttgttg
    29881 tctgagcctg cgcctggctc cgtcaacgcc agggcatagg cgggatcacc cgtttccagg
    29941 gtgctttctg ccgttttacg tagctgctct gcagaaccga aacggcgcat actgtgaata
    30001 cattgaccgt tggtgatcaa aaatgccgga gcaccgcatt ttgacacttc catcagcgcc
    30061 agcatttggg tgacgtaatc cgcagggata ccaccaaatt cttccggcac gccaagcatg
    30121 gaaataccgt tatccgccag cgcccgcata aactcacgcg gatatgtccc gttttgatcg
    30181 caggtgcgga aatactcttc cggaaagtta gtcgtaatca gttcgcgaat actggccagc
    30241 agcagttctt gttcttcagt taaagaaaaa tccatcatta tactcctgtt aatgagccgc
    30301 ttcaggcagg acaaaaccca tcgccgcatc gataaataat ctttcgtcca tcagtttgag
    30361 ttctggcgaa atcactgggg tgaaatccat tttgtcgaga atatcttttt gtaaatcgac
    30421 gccaggggcg atttcgatta aatgcaggcc gtcttctttc agcgtgaata ctgcgcgctc
    30481 agtgatataa cgaacatcca gccctcgctc gagagcgatt tttccgctga aagtaatttc
    30541 cggtagttcc cgaataaatt tcttcacccg tccttcctgg acgatattta atttgccgtc
    30601 ggtaatttct gtttttaaac tgcccgcagt taatgtgccg cagaaaatga ttttcttcga
    30661 agtggcactg atatcaataa atccaccggt gcccatgatt ttaccattga atttatgcac
    30721 gccgacgtta ccgtgctggt cgacttcagc aaaactcaaa taacaaacat ccagaccgcc
    30781 accgtgataa aaatcaaact gggacgtcat atccagaatg gcacgggtat tcacgttcgc
    30841 gccaaaggcg atcccctgtg aagtaattcc gccaatcgga cctgtttcaa ccgtcagaat
    30901 aaagtcatca gcacaacctt cttctcgggc gaccaggcca atgccgtcag caataccaac
    30961 gccgacattc cccaccgcgc ctttacgcat ttcgaataat gcgcgccgcg caactaattt
    31021 acgttggttt aggggcaggc taagtttggt actgtcatca agggtgaagt caccagaaat
    31081 aaagcggtta accggtgcac cgccatacag ttgggtttga tccggatcga ccaccacaat
    31141 atccaccaga taacccggaa tacggacaga tttaggatgc agtgtggctt tcttaaccat
    31201 tttctgcacc tgcatcatca caataccgcc gttattgtgc accgcctggg caataaccaa
    31261 tgcgtcgaga tacatcacct catcttcaaa agtggcgtag ccttcactgt cgcaggtggt
    31321 agcgcgaatg aaagcaatat ctggcgcaat cgctttgtaa tagagatatt ctttgttatc
    31381 aaactcgacc agtttaatca ggtcttcttt agtgacttca ttcagtttgc cgccatgctg
    31441 gcgtggatcg acaaatgtcc cgatgccaat atcactaata ataccaggct ggtgggctgc
    31501 ggcggcgcgt aaggtttgtg taagcacacc ttgtgggtag ttataagcaa taattttatt
    31561 ttgttctgcg agttcagaaa tacgcggcga ttgtccccag tgaccacata atgcccattt
    31621 caccagacct tcttgcgcca gaggactaat accgcggtcg gcgcgatcgc caagccctgt
    31681 tggactaata atcgataaat tacgtggcgt ttgagtctgt ttatatttat cagcaagagc
    31741 agtaattaac gtggtggctt ccagaatacc gccgccagcg cctaacacac aaagtgttgc
    31801 ttcgtcggga atataattca ccgcttcctg tgccgacagg accggcactc gtccattaat
    31861 acgaggtggt tttacaggtt tcattgttaa ttcctgattc agatgtgatt ttccctacgc
    31921 cagatggaga attagcgcac agagactcac gaaatatttt tattaaatac ggttaaatat
    31981 attttttaag ttcggcgggg agggtgttcc cgccgaaata ttattgctta tgcctggtgt
    32041 aaaatagtta ataccgtgcg caaatcattt accgagattt gccctggtgc agaggctttt
    32101 tttaccgcac caaaagttgc cgccgagcca aatacttcac cagccagacg agaaattacg
    32161 ccagtttttg ccatcgacat cgtgatgatt ggacgatcgg catactgctc ctgcatctcc
    32221 agggtcgcgg caagcaacgt cagcacatca ctggtacttt gcggcatcag cgcaatctta
    32281 ggaatatcgg cgtcgaagga ctgcattttg cgcagacggg caatgatttc ttcggcttcc
    32341 ggcgttttat ggaagtcatg gttggacatg acaactttca catcatgcgc gtgggcgtag
    32401 gcgacggttt ctttaacctg atcatcgccg gtaaataact ccagatcgat catatcaacc
    32461 aggccgctgt cgatggctgc acgattgagt gcaatataag cctcggtgga aatcgcctgc
    32521 tcgccgcctt ctttggcact gcggaaggta aacagcagcg gtttttctgg catggtttca
    32581 cggagaattt ttgctgccgc catgacagac tccacattgg agagatcggc atagtggtcc
    32641 acacgccatt ccagaatatc aaagtccgct tcacgatagg cgagagcttc ggatttcacg
    32701 ctggcgatat ctttcgccat cagcgagacg atgattttag gtgcgcccgt accaatgacg
    32761 agatctttta cagttacggt tttcattttt taccctttct gcacgcggtc agcctgtcag
    32821 gcaccgaacc ccatgacctg tttaacatat tccagaggga aatctttgcc agtccataac
    32881 gtgaactgtt cagccccttg ccacaacaac atgccgtatc catcaatcgt tttgcaacca
    32941 gcttgttgcg cctgctgcaa taacttcgtc atatgcgggt tatacacgca ttcagtgacc
    33001 agaagtcccg gatgtaatag actgatatca ttaactaatg attcattctc aaggggtttc
    33061 atacccactt ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc agcaaaggct
    33121 tgctgatcgg cgagatcggt aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc
    33181 gcgaaggcga gggctttatc gaagaactca tcccgacggt taaagagttt aatttctttt
    33241 aaaccttcaa ttgccccctg cgcgccaatc gccgttgagg cacctccggc ccctaacagc
    33301 accattgttt tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg
    33361 ccgtcggtgt tatagccgcg caggtagcca tcatcattaa cgatggtgtt gatggcaccc
    33421 accagtttgg ctgctggcgt taattcatca acatattcac acgccagttg tttgttcggc
    33481 attgatacac cagttccgcg catttttaga gcttttaatc cttcaattgc tccgggaaag
    33541 ctatcgttat ccacttcgaa ggccatatag gtaaatggca atcccgcttt ttctaaggct
    33601 ttattctgca tttcaggcga taaactgtgg cggataggat aagccatcaa cccaatcaat
    33661 tcgtattttg cggtaacatc cataattaac tcctttaacc tctatgctta attgtgttta
    33721 accgggtgct aaatttgcgc tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc
    33781 ggtaatagcg gataaaaaca ataattgcgg taatcagggc cagcagcgcg aaagtaaaat
    33841 cgagaacaat gatatattgc aggccgatgt tcgacagata accagtaatc agcggaataa
    33901 caaagttagc cagtccaccc atcatcatat aaatactggt gactttggct ttgctttttg
    33961 ggaaaaactc tgacatgacc gaaacaccga gctgtaaaat gccgccagct gctgagaaac
    34021 cgataacaaa ggcgccggca ttacacacca gtggagaagg gtacaggtag ataatggctg
    34081 ctgttattgt tgccagtgca gagttaaata cattagccca gatgggccgg accatttttt
    34141 tcagtagtgc ggcaaaaata aagacacaga ccaacgagcc catactgtaa taagagatgg
    34201 tttttaatgc ctcagcttct gacataccag caaaagccat cgcatatttg ggcatccaca
    34261 ccacaatcac ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt
    34321 ccagccagac taacggtttg ctgttcattt gcggtaattc attagctacg ctggcgtcca
    34381 ccaactggct ggggaatttg cttttcaaca gcatcagcgt gatcagtaca aacagaatac
    34441 ccggaataat cagcccatag ccgtaccaga tattattgag caacatatag ctcaccagca
    34501 ttgggtagaa catttgcccg aatgacacca tcgctttaac cagtatgacc gccgaaccag
    34561 aggctttcgg aaagcattcc atgagcgcgg ggtagccacc cgtatccagc gctgagttag
    34621 cgatacctac gcacactgcc agaccgtagg cgagagttaa attcgggcaa gcgggaatac
    34681 caaagaagaa tagcagatac attattactg ccattaatat caccgcccga cgaccaaact
    34741 tatcggagat cacaccgaag aataaaatac tgatcaatcg ccccaaaccg ataccggaaa
    34801 ttaagtaggc aatgcccgcg ttgtcagtgg aaaacttttc cgccagagat gacatatttt
    34861 gggcaagcgt aataacacta atgccgtgca ggaagtagct gaagtaaata caaagaacag
    34921 ccaggataaa tggcgtgctg aaagccttat tttgagacat tttcacagct ccttgctgaa
    34981 agataatttc gttaccgtac gtattgttct ccgacggttt acataattac tatactgaag
    35041 atgattttcc ctgggtacat ggcataaagt ttatgtttcc aggaatattg catgtcatag
    35101 tttgtttttg atcgcgattt tccttagtta gcgcagaaag agttatttat ccgtaagatc
    35161 ttgcatgacc tgaaaagaat attgcattac ccacccggag cgacattttc ctttagcgag
    35221 tgatgccgcg ttttattgcg gctgcgtaag ccgataaaca gtgccagtaa aaagccgatg
    35281 gcagcgatgg cggtatcgaa ccacataata tcggcaatac ttctttggga cagatgagcc
    35341 gtaatcaacg gaatagtaaa ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct
    35401 ttacctttag cgtaagggaa acgttcagcc attaacgtca agccaatttg cacaacacct
    35461 ccagcagagg taaaaccaat gacaaaagca aatattatca ccacataaaa tgtgggatgc
    35521 aggcagacgg taaacagagc aataaatgag ataaaggtgt acagcatcag taatgttgtt
    35581 gggcgaacgg tattacgaat gagtggagcg gtaataaata cacaaagcag cgaacccacg
    35641 gtgtagatac tgagtagttt gatcgacata gtgtatgaca tgcctgcaac aaattgtccg
    35701 tactgtgcca gccactggct aaccagataa aacgttgcca ttgagatata gccatataag
    35761 gtataactgg ctaaatcgat aattgaacag cgatgttccg tagagctggt ggttttcttt
    35821 atgacaggtg agcgacgacc cggatggggt gggaacgtac aacggtataa aaacagagcg
    35881 ttaataaaca taatgcctgc agcaatcata aaggaccaac cgaaccacag ttcagcccac
    35941 actaacaggc taatgattag cggtaataaa aattgtccgc tggaaacaaa tgctttaatt
    36001 aaaatattgg ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg ataagtgcct
    36061 gcatcgagaa aactgtttgc cattcccgcc agaaagccaa aaacataagc gataatgatg
    36121 ttattggtgt gcaggatgcc aaaaaagaag gccatatagc agcacatccc gagcatgata
    36181 aaagggcggc gaccaaagcg atcggataat aatcctgcaa aaagcaagac acttaatcga
    36241 ccaatgccca gcgatgagat aactatcgag acacccgcgg cattagtctg ccaaagtgtc
    36301 tccagcgagg ccatattcag gctcatcaaa atgacgccca taccatgcac caagtaatta
    36361 aaatacaacc caagtgcggt agggtaatag ggatttttca taggttctac ttagcaaagg
    36421 tgctttaaaa agtgtagcga tgttgtgtca gtatccgttc attattgttg taacaacgac
    36481 aattcctttc tgtggaaaaa ttaacaaagc cgctccagat cgtgtgcata taattcagcg
    36541 gctaccgatt gatagatctt ccagtcaata aaattgccat cagggtagac ttgttgaaac
    36601 gcggttaagt cggggaaaaa acgcatggtg ttgttgccga tgcggttatt tattttctct
    36661 atgatggcat gaagatgttt ttttctttgc tgacgcatag ccaacagttg ctcgactaca
    36721 caatcaggaa tggcacactt gccattttcc cattgacgcc agctttcact gtcaccggtc
    36781 tgggcaatcc aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg gagtgcctgg
    36841 agttcataag cgttcatcgt gtaatggctg catgaa
//
LOCUS       DAPGRN010000039        36783 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPGRN010000039 DAPGRN010000000
VERSION     DAPGRN010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.039"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            272..2977
                     /gene="malT"
                     /locus_tag="RHW33_002104"
     CDS             272..2977
                     /gene="malT"
                     /locus_tag="RHW33_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV1645604.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            3044..3322
                     /locus_tag="RHW33_002105"
     CDS             3044..3322
                     /locus_tag="RHW33_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV1645605.1"
                     /translation="MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACRE
                     AQDILLDQRLFILDDEQYDAFLAALDAPITAERQAKINALMNRKSPWE"
     gene            3313..3798
                     /locus_tag="RHW33_002106"
     CDS             3313..3798
                     /locus_tag="RHW33_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1645606.1"
                     /translation="MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRV
                     YVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAPESLPVVVLGRLAIDQAWAGKGL
                     GVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVSSPFQSLTLQYPITL
                     E"
     gene            complement(3848..4876)
                     /gene="rtcA"
                     /locus_tag="RHW33_002107"
     CDS             complement(3848..4876)
                     /gene="rtcA"
                     /locus_tag="RHW33_002107"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV1645607.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKMGIHQQTTLLRHGFY
                     PAGGGVVATEVSPMASFKTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAASFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGQFTVAHPSCHLLTNIAVVERFLPVRFTLAETDGVTR
                     VMITKLTD"
     gene            complement(4880..6106)
                     /gene="rtcB"
                     /locus_tag="RHW33_002108"
     CDS             complement(4880..6106)
                     /gene="rtcB"
                     /locus_tag="RHW33_002108"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV1645608.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDGSDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            6293..7891
                     /gene="rtcR"
                     /locus_tag="RHW33_002109"
     CDS             6293..7891
                     /gene="rtcR"
                     /locus_tag="RHW33_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV1645609.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFASSGRITLEVVEDEINRLRYNWQES
                     RPSALTQLLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASINDA
                     DRLRKYLARFGLTWAALQDQHSSS"
     gene            complement(7873..8631)
                     /locus_tag="RHW33_002110"
     CDS             complement(7873..8631)
                     /locus_tag="RHW33_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV1645610.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(8648..9478)
                     /gene="glpG"
                     /locus_tag="RHW33_002111"
     CDS             complement(8648..9478)
                     /gene="glpG"
                     /locus_tag="RHW33_002111"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV1645611.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(9523..9849)
                     /gene="glpE"
                     /locus_tag="RHW33_002112"
     CDS             complement(9523..9849)
                     /gene="glpE"
                     /locus_tag="RHW33_002112"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV1645612.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            10039..11544
                     /gene="glpD"
                     /locus_tag="RHW33_002113"
     CDS             10039..11544
                     /gene="glpD"
                     /locus_tag="RHW33_002113"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV1645613.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLNVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAVSDLGEDFGHEFYETE
                     LKYLVDHEWVRRADDALWRRTKQGMWLSAEQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(11738..12340)
                     /locus_tag="RHW33_002114"
     CDS             complement(11738..12340)
                     /locus_tag="RHW33_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645614.1"
                     /translation="MMNIIRLYIYPLFLGFVIVPLLVWPMVVALAACIITLSFLGEML
                     FSIPLMIRRISLLQLQLWLSAEYALFFCVMCCVGWQFTRRTQSELKSRLHCWLVFAPV
                     YLWLILWNIIFYIAPAKIALLESIRNFFLLVVWLPLNFSPFWPQPWTDFIGPISAQLG
                     FALGYYCQWRSKNRCQIRKWSEWGTCLSFVTLGMMAWALR"
     gene            complement(12340..13332)
                     /locus_tag="RHW33_002115"
     CDS             complement(12340..13332)
                     /locus_tag="RHW33_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1645615.1"
                     /translation="MNKFTGVLLLGAALLAGCVDREGYYNSVREEESHGLTSLRGQPA
                     LRYSDDWSRWPRVYGATALYPLYASAYYKLVPEPKDKDRTSLAWQAYGLQQTRTAEAY
                     DSLIKGSATVIFVAQPSEGQKKRAEEAGVKLKYTAFAREAFVFIVDINNPVNSLSEHQ
                     VKDIFSGKTSRWNKVGGSDEHIKVWQRPEDSGSQTIMKGLVMQDTPMLPAKKSTVIDL
                     MGGLITEVADYQNTPSSIGYTFHYYVTRMNDNMLKMRKQIKLLAINGVAPTEENIRNG
                     TYPYIVDAYMVTRENPTPETQKFVDWFISQQGQQLVEDVGYVPLYEASPESSGQ"
     gene            complement(13358..14866)
                     /locus_tag="RHW33_002116"
     CDS             complement(13358..14866)
                     /locus_tag="RHW33_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1645616.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTIYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPALLIL
                     VGWIIALHLADYQFRQMGSGWLADLMLPWLGVLLVSLVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTWEYLE
                     NSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFARESFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASVMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGITPTAENI
                     RNGKYAYIVDAFMVTRENTTSETQKLVEWFLTPQGQSLVEDVGYVPLYPTME"
     gene            complement(14991..17438)
                     /gene="glgP"
                     /locus_tag="RHW33_002117"
     CDS             complement(14991..17438)
                     /gene="glgP"
                     /locus_tag="RHW33_002117"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV1645617.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(17457..18890)
                     /gene="glgA"
                     /locus_tag="RHW33_002118"
     CDS             complement(17457..18890)
                     /gene="glgA"
                     /locus_tag="RHW33_002118"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV1645618.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMANGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(18890..20185)
                     /gene="glgC"
                     /locus_tag="RHW33_002119"
     CDS             complement(18890..20185)
                     /gene="glgC"
                     /locus_tag="RHW33_002119"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV1645619.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(20203..22176)
                     /gene="glgX"
                     /locus_tag="RHW33_002120"
     CDS             complement(20203..22176)
                     /gene="glgX"
                     /locus_tag="RHW33_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV1645620.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(22173..24359)
                     /gene="glgB"
                     /locus_tag="RHW33_002121"
     CDS             complement(22173..24359)
                     /gene="glgB"
                     /locus_tag="RHW33_002121"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV1645621.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(24632..25735)
                     /gene="asd"
                     /locus_tag="RHW33_002122"
     CDS             complement(24632..25735)
                     /gene="asd"
                     /locus_tag="RHW33_002122"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV1645622.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            25927..26520
                     /gene="yhgN"
                     /locus_tag="RHW33_002123"
     CDS             25927..26520
                     /gene="yhgN"
                     /locus_tag="RHW33_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV1645623.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(26566..27861)
                     /locus_tag="RHW33_002124"
     CDS             complement(26566..27861)
                     /locus_tag="RHW33_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV1645624.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDKQSANSQLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(27768..29864)
                     /locus_tag="RHW33_002125"
     CDS             complement(27768..29864)
                     /locus_tag="RHW33_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HDV1645625.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRGIDSAVGILLCGSEDRAIHKFFRESAFPAEAQIHEILNVLSVNDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELAD
                     VESYITTKECKMQFLRRALDEPGAEHCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVAAGAFTQYGFKGNLPASLQGSTGRILSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(30004..31344)
                     /gene="gntU"
                     /locus_tag="RHW33_002126"
     CDS             complement(30004..31344)
                     /gene="gntU"
                     /locus_tag="RHW33_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV1645626.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISDPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(31348..31875)
                     /gene="gntK"
                     /locus_tag="RHW33_002127"
     CDS             complement(31348..31875)
                     /gene="gntK"
                     /locus_tag="RHW33_002127"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV1645627.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(32014..33009)
                     /gene="gntR"
                     /locus_tag="RHW33_002128"
     CDS             complement(32014..33009)
                     /gene="gntR"
                     /locus_tag="RHW33_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV1645628.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(33233..33928)
                     /gene="yhhW"
                     /locus_tag="RHW33_002129"
     CDS             complement(33233..33928)
                     /gene="yhhW"
                     /locus_tag="RHW33_002129"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV1645629.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(34051..35088)
                     /locus_tag="RHW33_002130"
     CDS             complement(34051..35088)
                     /locus_tag="RHW33_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1645630.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLNEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLVK"
     gene            35422..35910
                     /gene="yhhY"
                     /locus_tag="RHW33_002131"
     CDS             35422..35910
                     /gene="yhhY"
                     /locus_tag="RHW33_002131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1645631.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGTVVGHLTIDVQQRPRRSHVADFGICVDARWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYFMAR
                     VK"
     gene            36121..>36783
                     /locus_tag="RHW33_002132"
     CDS             36121..>36783
                     /locus_tag="RHW33_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4225 domain-containing protein"
                     /protein_id="HDV1645632.1"
                     /translation="MGTAAISHLRYDLNKYALSLRKTATLASTFFIESPLVRFEYLQE
                     IENTINDITHRFNSSYDINEKARLINELKMESETARKEYQLFRQGNYDKYITTDIFEE
                     HGLIKYVNLSFDIVASVGQVVGGVGALKFGKVVHSNRIKGIGVTLVAHGANNFYESLS
                     PLFFNEYDSGPIRELYRVIAKKMGGDINSSDYAYSIVDFSITAYGGYSGMKIVPKYNR
                     LIR"
BASE COUNT         9186 a         9828 c         9000 g         8769 t
ORIGIN      
        1 ctttttagat agcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat
       61 gaacataagg aaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata
      121 aagatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca
      181 ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat
      241 ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaat taagtcgtcc
      301 ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa
      361 caacttccgg ctggcgctga tcacaagtcc tgcgggctac ggtaagacca cgctcatttc
      421 ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggacgaag gtgataacca
      481 gcaagagcgt ttcgccagct atctcattgc cgccgtgcaa caggcaacca acggtcactg
      541 tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc
      601 ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta
      661 tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc
      721 agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct
      781 gcgtgttcgt gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga
      841 agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag aaagcagtcg
      901 gatttgcgat gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg
      961 gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca
     1021 tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt
     1081 tctgttgaaa agcgccattt tgcgctcaat gaacgatgca ctcatcaccc gtgtgaccgg
     1141 cgaagaaaat gggcaaatgc gcctcgaaga gattgagcgt caggggctgt ttttacagcg
     1201 gatggatgat accggcgagt ggttctgcta tcacccgctg tttggtaact tcctgcgcca
     1261 gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag
     1321 ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc
     1381 gctgatgctg cgcgatattc tgcttaatca cgcctggagt ctgttcaacc atagcgaact
     1441 gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agcttgctgg aaaatccgca
     1501 gttggtgtta ttgcaggcgt ggctgatgca aagccaacat cgctacggcg aagttaacac
     1561 cctgctagcc cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga
     1621 atttaacgct ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg
     1681 gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc
     1741 aacctcggtg ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct
     1801 aatgcagcaa accgaacaga tggcacgcca gcacgacgtc tggcactacg ccttatggag
     1861 tttaattcag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac
     1921 gcaggaaaaa gcgttccagc tgatcaacga gcagcatctg gaacagttgc cgatgcatga
     1981 gtttctggtg cgcatccgag cacaactgtt atgggcctgg gcacggctgg atgaagccga
     2041 agcgtcggcg cgtagcggga ttgaagtctt atcgtcttat cagccacagc aacagcttca
     2101 gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag
     2161 ccagttgaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc
     2221 taacgccaac aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc
     2281 caactggttg cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca
     2341 atggcgcaac attgcccgcg cacaaatctt gctgggcgag tttgaacctg cggaaattgt
     2401 cctcgaagaa ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa
     2461 cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgtgt
     2521 gttgctggac gcattaaaac tggcgaatcg caccggattt atcagtcatt ttgtcatcga
     2581 aggcgaagcg atggcgcaac aactgcgcca gctgattcaa ctcaatacgc tgccggaact
     2641 ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt
     2701 cgcccatttc gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact
     2761 gatccgcacc agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg
     2821 ttacagcaac gaacaaatag cgggtgaact ggaagtggcg gcaaccacca tcaagacgca
     2881 tatccgcaat ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg tgcagcacgc
     2941 gcagaaactg ctgaagatga tgggttatgg ggtgtgagtt tgtatagcgt ttttatgtac
     3001 actctttata ctgtatataa aatcgcacta caggctcatg aatatgaaac cggaaagcaa
     3061 agaagcgcca atcaatatca gagcgaaagc ctcacagcgt gacctgattg atatggcagc
     3121 gaatctggtc gcaaaatcgc gcactgattt tatgctggat gccgcatgcc gtgaagcgca
     3181 agacatctta ttagatcaac gactattcat ccttgatgac gagcaatacg atgcgtttct
     3241 cgctgccctg gacgccccca tcactgctga gcgtcaggcc aaaattaacg cattgatgaa
     3301 tcgtaaatcg ccatgggaat aacggctcct actcccctaa cgtctgaaca taatctggct
     3361 gacttttgct gttccgatca cgggatgaat gaatggctaa aaaagaaagc gttaaaaaat
     3421 cacagctccg gtctgtctcg tgtttacgtt atctgtattg ccaatacccg tcaggtaatc
     3481 ggctattact gcctttctac aggcagtatt caacgtaatt tggcccctgg agccatgcga
     3541 cgtaatgctc ccgaatcgtt gcccgtagtc gtattaggca gactggcaat tgatcaagcc
     3601 tgggcaggta aagggttggg agtcgcattg ttgaaagacg cagtctatcg cacaatgtcc
     3661 atagctcaac aggtaggggt tcgtgcgctc attgttcatg ccttagacga ttcagtacgc
     3721 aatttctatc tgaagtatgc ctttgtatct tcaccattcc agtcacttac cttacagtat
     3781 cccattactc tggagtaatt tacctctacc ggatagtcac actgatgagt ctgatccggg
     3841 agggatttta atcagtgagt ttggttatca tcacccgcgt tacgccatct gtttcagcca
     3901 gagtaaaccg caccggcaag aaacgctcca ccacggcgat attggtcagc agatggcatg
     3961 agggatgggc gaccgtaaat tgtcccgcgc ccgccagcgc cattggcaac actagctggt
     4021 cagcaaggta ttcccccact gccgccgggc ttgccaggta gcgtttcacc tctttcacca
     4081 actgtgccgc aaccacctcg gcactgacgc gcttttcacc gacgacaaaa aagcgttcgg
     4141 tgatattttc actttcgact tcaagcgaga cggtattacc cggcccctgg tcgcgcggca
     4201 ggttatgaat attctgctca tgcagggaaa aacttgccgc cagcgtagcg atttcacgct
     4261 cagcgacatg gcgcggcacg ccagctaata acacctctcc acgcatctgc acaatgttcc
     4321 cgcgctcgcc aagttgcaag gttttaaacg atgccattgg cgagacttct gttgccacca
     4381 cgccgcctcc ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaattccca
     4441 ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggtggg gccgacgggt
     4501 tatcggtgcc tccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca
     4561 gcaccgtttg cagcaccagc gtacagcttc cggcactacc gatagcaaag cggtaatcgc
     4621 cgccgcgcac ggtgccaggc cggaagagca gacgctgcga tcccagctcc gcgccttcca
     4681 ccgttgccct acaaatttcc gcagctgctt ttaccgcggt cagatgctgg cgcaacagtc
     4741 ccggttttgc ccgcccggca cgaatgccgg tgatggtaaa tggctggccg gttatcatcg
     4801 acagactcag cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg ccatccagcg
     4861 caatcatcct tttcatcatt tatcctttta cacacaccac ctgacgcagg gtatagataa
     4921 cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga
     4981 tttcgtcgat cacttcggca tctttacggc attctacatg cgcggtggca cgaatttgat
     5041 cttccacgct gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc
     5101 tgcacgaaca gaacgactct tcatttccca gtccacgaac gataaagctt tttgccccca
     5161 tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca
     5221 catagatctc ttcaccaaag tgctgttctt tttgcacata gttgtggtga caattgatct
     5281 cttccatcgc cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg
     5341 ttactacgtt ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaggcca
     5401 cggctttcag gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg
     5461 acggcaatgt ttcaagcgtt tcctgcatct ctttttgcgc cagatcgata aagtaagtcc
     5521 cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc tgatccgacc
     5581 catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg tgtttatagt
     5641 tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg gcctccagct
     5701 cagcccattt cgcatcgacg ttaacaggcg ggttttccca tgcgccttta tcacgtttac
     5761 aacgtccggt agtacgcccg tgcggcacgg ccgtttcaat cgcctgacgc agctccgcca
     5821 ggttttcagg caggtcagcc gccgttaacg cggtacgcag cgcgttcatt ccacagccaa
     5881 tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg
     5941 tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca
     6001 tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc
     6061 acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt
     6121 ttcgttggta tgtcctgata attgcaaccg tcgtgccaga aaattaaata agcagattaa
     6181 ttttttaatt cattgtttta aaaaagatta tatctttacg tccgtaaccg gagatttccc
     6241 gcaaagccga tttaccgata atgaaatatc gtcttttata aggatatcta agatgcgtaa
     6301 aacagtggct tttggctttg tcggaacggt gctggattat gccgggcgcg gcagtcagcg
     6361 ctggtcgaaa tggcgtccga cgctttgttt atgccagcaa gaatcgttgg tcatcgatcg
     6421 actggaatta ttgcatgacg cccgctcgcg ctcgctattt gaaacgctta aacgcgatat
     6481 cgccagcgtt tcgccagaaa cagaagtggt gagcgttgag attgaactgc ataacccgtg
     6541 ggatttcgaa gaggtttacg cctgcctgca tgatttcgcc cgtggttacg agtttcagcc
     6601 agaaaaagaa gactatttaa ttcacatcac caccggcacc cacgtggcgc agatttgctg
     6661 gtttctgctg gcggaagcgc gttacctgcc cgcccggctg atacaatctt cacctccgcg
     6721 caaaaaagaa caaccgcgcg gcgcaggcga agtgacgatt atcgatctcg atctaagccg
     6781 ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg attttcttaa
     6841 gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga ttgaaaaagt
     6901 ggcgattaaa tcccgcgcac cgatcctgct taacggcccg accggggcgg gtaagtcatt
     6961 tctggcgcga cgcatctttg agttaaaaca ggcgcggcat cagtttagcg gtgcgtttgt
     7021 ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt ttggtcatgt
     7081 gaaaggcgct tttaccgggg cgcgggaatc gcgtgaaggc ttattacgca gcgccaacgg
     7141 cggaatgttg tttcttgatg agatcggcga actgggcgca gacgaacagg caatgctgct
     7201 taaagccatt gaagaaaaaa ccttttatcc gtttggcagc gatcgccagg tgagcagtga
     7261 ttttcagctt atcgccggaa cggtgcgcga tttacgccag ctggttgccg aaggcaaatt
     7321 ccgcgaagat ctatacgcgc ggatcaatct ctggaccttc accctgccgg ggctacgcca
     7381 gcgccaggaa gatattgaac cgaacctgga ttatgaagtg gagcgccacg cttcactcac
     7441 cggcgacagc gtgcgtttta acaccgaagc gcggcgtgcc tggctggcct ttgcgacatc
     7501 tccccaggca acatggcgcg gtaattttcg cgagctttct gccagcgtca cgcggatggc
     7561 tacctttgcc tctagcggac gcattactct ggaagtggtt gaagatgaga taaaccgtct
     7621 gcgctataac tggcaggaga gtcgcccctc cgcgcttacg cagctactgg gcgcagaggc
     7681 agaaaacatc gatctcttcg accgtatgca actggaacac gttatcgcaa tctgccgcca
     7741 ggcaaagtcg ctttctgcag ccggacgaca gctttttgat gtttcgcgcc agggcaaagc
     7801 cagcatcaac gacgcggatc ggctacgcaa atacctggcg cgttttggtc tgacgtgggc
     7861 agcattgcag gatcagcaca gctccagttg aatatggtgg tccgtcagca cctgcatcac
     7921 gcttactggc ggaggagcgt cggtgtagac ggcatcaacc atgctgatgc tacccatatt
     7981 gaccatcgcg ttacggccaa atttcgagtg atcgaccacc agcataacgt ggcgcgagtt
     8041 ctcaataatg gcgcgtttgg tgcgaacttc gtggtaatcg aactccagca gcgagccgtc
     8101 gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg agataaaatc
     8161 gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac cggcgagaat
     8221 aatgcggaaa tcttctttta ccatcaacgt gttagcgacg ttgagattgt tggtgacaat
     8281 acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg tgccaatatc
     8341 gataaacagc gtcgagccgt tggggatttg ctccgccact ttacgggcga tgcgctcttt
     8401 ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg ttaaccgaac tggaaggcag
     8461 cgccgcaccg ccatgatggc gcaggatcag gttttgctcc gccagctcat tgaggtcgcg
     8521 gcgaatagtc tgcgggctga cggagaagtg ctccaccagc tcctcggtac tgacataacc
     8581 ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca tttctaaatc
     8641 cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc gctaaaccca
     8701 cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa tcaaaccatc
     8761 cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa atgccacttt
     8821 gcggatcgcg ttcgccacgt agccagacgt agcccatcag cgcatacacc acgccagaaa
     8881 gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac agggcgctaa
     8941 tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgcg ccgccgagat
     9001 accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt aacgcgtggg
     9061 tgaagtaacg ccagaactca aatttcagtg ctggatcgaa tggccaggcc agccataaca
     9121 tcacttcctg atcgccgaga atttgcatgg caataaacac caccacgcag gcgatcatca
     9181 tcacccaggt taccggacct gcgcgttcac gcaaggcggc aaagaaagga taacggcgat
     9241 aatgcaggcc actgccggta tggcctgcct gccagctcgc cgccagataa cgcggatctg
     9301 ccgggttttc gagaaaacgc gccagctccg cccgtacgcg ctcggcctgg gactcatccg
     9361 ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc tgcgtcgcca
     9421 tgtaatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc atcaacatcg
     9481 ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc cgtacgccac
     9541 ctctgcggga aactgacgtt gccaggcttc aaagccgcca tcaatgctat agaccacatc
     9601 gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattaccgt ggtaacacat
     9661 caccatcacc ggtgtgtcaa agtcgttatc acgcataaac gcgcccagtg tgtcgttggt
     9721 taaatggaaa gcctgcaccg catgccccat tgcgaaactc tgtggatcgc gaatatcgac
     9781 cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa tacattcgaa
     9841 ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc acagtttacg
     9901 tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt tttttcaatg
     9961 ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg agctttaacg
    10021 aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg cggcatcaat
    10081 ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat gctggaggcg
    10141 caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg tggcctgcgc
    10201 taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg tgaagtgctg
    10261 ctgaaaatgg ccccgcatat cgccttcccg atgcgttttc gcctgccaca tcgtccgcat
    10321 ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct gggtaaacgc
    10381 accagcttgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt aaaaccggaa
    10441 attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct ggtactcgcc
    10501 aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg cgccacctct
    10561 gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac cggcaaaaaa
    10621 tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa acagttcttc
    10681 gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg cagccatatt
    10741 gtggtgccgc gcgtgcatac ccaaaagcag gcctacattc tgcaaaacga agataaacgt
    10801 attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac cgatgtggaa
    10861 tacaaaggcg atccgaaagc ggtgaagatt gaagagagtg aaatcaatta cctgctgaat
    10921 gtgtataacg cgcactttaa aaagcagtta agccgtgacg atatcgtctg gacctactcc
    10981 ggtgtgcgac cactatgtga tgatgagtcc gactcgccgc aggctattac ccgtgattac
    11041 acccttgata ttcatgatga aaatggcaaa gcaccgctgc tgtcggtatt cggcggtaag
    11101 ctgaccacct accgaaaact ggcggaacat gcgctggaaa aactaacgcc gtattatcag
    11161 ggtattggcc cggcatggac gaaagagagc gtgctaccgg gtggcgccat cgagggcgac
    11221 cgcgacgatt atgccgctcg cttacgccgc cgttatccgt tcctgaccga atcgctggca
    11281 cgtcattacg cgcgtactta tggcagcaat agcgagctgc tgctcggcaa tgcgggagcg
    11341 gtaagcgatc ttggggaaga tttcggtcat gagttctacg aaacggagct gaaatatctg
    11401 gtcgaccacg agtgggtccg ccgcgccgac gatgccctgt ggcgtcgcac aaaacaaggt
    11461 atgtggctaa gtgcggaaca acaatctcgt gtgagtcagt ggttggtgga gtatacgcag
    11521 cagaagctgt cgctggcgtc gtgatttaat gtaaggtggc caggtcaaat ttcaatctga
    11581 cctgagactg atgacaaaca caaaattgcc tgatgcgtgg tttatgcaat atattgaatt
    11641 tgcatgattt tgtaggcctg ataaggcgtt cacgccgcat caggcatgaa caaagtgcac
    11701 tttgtcaaca atctgaccta ctttttgagt cagggaatta ccgcaaagcc cacgccatca
    11761 tacccaacgt aacaaaactt aagcacgttc cccactcact ccacttccgt atctggcatc
    11821 tatttttgct gcgccactgg caataatatc ccaacgcaaa accaagctgt gcgcttattg
    11881 gaccgataaa atccgtccac ggctgaggcc aaaatggcga aaaattcagg ggtagccaga
    11941 caacaagcag aaagaaattg cggatacttt ccagcaacgc aattttcgct ggcgcgatgt
    12001 aaaaaatgat attccagagg atcaaccata aatagaccgg tgcgaatacc aaccaacaat
    12061 ggagcctgct tttcaattca gattgagttc tacgagtaaa ttgccaacca acgcaacaca
    12121 ttacacagaa gaaaagcgca tattcagccg ataaccaaag ttgtaattgc agaagtgaaa
    12181 tacgcctgat catgagcggg attgagaaca gcatttcccc cagaaaactc agcgtgataa
    12241 tacaagcagc cagcgcaacc accatcggcc acaccaacaa cggcacaata acaaagccga
    12301 gaaataacgg ataaatataa agtctgatta tattcatcat tattgtcctg atgattcggg
    12361 ggatgcttca tacagcggca cataccccac atcctctacc aactgttgcc cctgctgact
    12421 tataaaccag tcaacaaatt tctgcgtttc cggcgtggga ttttcacgcg tcaccatata
    12481 ggcatccaca atgtatggat aagtgccgtt gcggatattt tcctcggtag gcgcaacgcc
    12541 atttatagcc aaaagtttaa tctgcttgcg cattttgagc atattgtcat tcatacgagt
    12601 gacgtaatag tggaaggtgt acccaatgga agatggcgtg ttttgatagt cggcaacttc
    12661 agtaattaaa ccgcccataa gatcaatcac agtggatttt ttagctggca gcattggggt
    12721 gtcttgcata accaacccct tcataatcgt ttggcttcca gaatcttcag ggcgctgcca
    12781 gacttttata tgttcgtcac taccacctac tttattccag cggctagttt tgccgctaaa
    12841 aatatcttta acctggtgct cagagagaga atttaccggg ttattaatat caacgataaa
    12901 gacaaaggct tcgcgggcga aagcggtata tttcagttta acacccgctt cttctgcacg
    12961 ttttttctgt ccttccgacg gttgtgcaac aaaaataacc gtcgcggaac ctttaatcag
    13021 actatcgtag gcttcagctg ttcgggtttg ctgcaaacca tacgcctgcc aggccagcga
    13081 ggttcgatcc ttatcttttg gctcgggtac taatttataa tacgcggagg catacagcgg
    13141 gtataaggct gtagcgccgt acactctcgg ccatcttgac caatcatcgc tgtaacgtaa
    13201 tgcaggttgc ccccgcagag acgtcagtcc atggctctct tcttccctga cgctgttata
    13261 gtacccttcc cggtcgacac atcccgccag taacgccgcg cctaatagta aaacaccggt
    13321 gaatttattc atctcgctga ttcctttctc tcgagcgtta ttccattgtt ggatacagcg
    13381 gcacatatcc cacatcttct accagactct gcccctgcgg cgttaaaaac cattcgacca
    13441 gtttttgtgt ttctgacgtt gtattttctc tcgtcaccat aaatgcatcg acgatgtacg
    13501 catatttgcc gttgcgaatg ttttccgccg tcggtgtaat accgttaatc gctagcaatt
    13561 ttatattttt atcagcattc atttgcgtcg cgtaatagcg gaaggtatag cctattgcgt
    13621 tgtttgtatt acggtattcg gcaacgactt taatcattcc ctccatcacg cttgccactt
    13681 ccgtttcctg cggcgagatc atgcggacat ttttcatgac ctgtgattgc atcactgtct
    13741 ggctgccaga gtcttccggg cgctgccagg tctggatctc ctgatcgtta ccgccaacag
    13801 tacgccagtt ggtaattgca ccactgaaaa tgtcacgcac ctgttgttca gtcagggaat
    13861 taaccggatt atccgcattg acgatgaaaa caaatgattc acgggcaaat ggcgtgtata
    13921 gcaaagtgac gcccgattcc tcagcgcgtt ttttctgccc accggagggt tgcgccacaa
    13981 aaataatatc ggcatcacct ttaacaatcc ggttatatgc atcgggggta cgagagttct
    14041 ccagatattc ccaggtgtgg aaatcttccg gtataacgct caacgcataa aaggcagagg
    14101 cataaattgg gtacgccgcc gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa
    14161 tttgcggttt accacgcagt ggtgtcagtt gattattgag tttatcgggt cgccaggccc
    14221 aggtatcaat attttcgcgc acaccgacgc cgggattgag ctgtttatat aaataaccct
    14281 gataaccagc gacaaaacca agcatgagaa ggataaacag gagggaatta cggcaacgta
    14341 gcccggatgt accggttaaa ggatgtctgg ttggccggcc gtaccccagc gaaaaactga
    14401 tatgtgcgcc aacgggtata atgactatcc accagtactc acctccgact aatgagacta
    14461 acaaaacgcc cagccagggg agcataagat cagccaacca acccgatccc atctggcgaa
    14521 attgataatc tgccagatgc agcgcgatta tccagccaac caggattaat aaagcgggta
    14581 ctaaaaaaag acattgtcgc cagacggcta tttctctttt ttctggtgca ctatcgcctg
    14641 cccatactcg ccccgagaaa aatagcatta atgcgcccag cgccagcata aaaatgttga
    14701 gcagaaggta aattgttggc gtgataagga tattacaaac ttcaataata ccggcaagtc
    14761 cgacacccag catagcaaca accgccgcca aaaattgcgt cagtatgggg atgccgaaaa
    14821 aagtcattac cagcgaggac aaaatccatt ttctgttttg cattattctt tccattcttt
    14881 ctgaatggcg aaattatact ccccgagtcc ccttgcccct tctggacact tttccgaaat
    14941 gatggcgaaa aaaacgggac cctttggccc cgttctgttt tttagcgaac ttacaatctc
    15001 accggatcga tatgccagat atgatcagcg tactctttga tagtacggtc agaagagaag
    15061 tagcccatat tggcaatgtt cagcatcgct tttgcggtcc actcttcctg aagctcgtag
    15121 agttcatcga ctttatcctg acaatcgaca tagctgcgat aatccgccag tacctggtag
    15181 tgatcgccga agttgatcag cgaatcaacc agatcgcgat agcgacccgg atcttccgga
    15241 ctgaatacac cgctgccgat ttgcgtcagc acctgatgca gctcctcatc tttctcatag
    15301 tattcacgcg gtttgtagcc ctgacgacgc agttcttcca cttcttccgc tgtgttacca
    15361 aaaataaaga tattgtcagc accgacatga tccagcatct cgacgtttgc accgtccagc
    15421 gtaccgatag tcagcgcgcc gttaagcgca aacttcatgt tactggtgcc ggaagcttcc
    15481 gtccccgcca gcgaaatctg ttcagacaga tcggccgccg gaatgatcaa ctgcgccagg
    15541 ctaacgctgt agttcgggat gaacacgact ttcagcttgt cgccaatctg cggatcgttg
    15601 ttgatcactt tcgctacgtc attgatcaaa tggataatat gcttcgccat gtaataggcc
    15661 gaagccgcct taccgccaaa aatattcacg cgcggtaccc acttcgcatc cgggtcggcc
    15721 ttgatgcggt tatagcgggt aatcacatgc aacacattca tcaattgacg tttgtattcg
    15781 tgaatacgtt tgatttgtac atcgaacaac gcctttggat tcaccaccac attcagctgc
    15841 tgggcgatat actccgccag acgctttttg ttctccagct tcgcctgatg cacagcgtga
    15901 ttaaccattg ggaaatcaca gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc
    15961 cagttacggc ccaggtgttc gtccagcacg gctgaaagcg atgggttcgc taccgccagc
    16021 cagcgacgcg gcgtcacacc gttggtgacg ttggtgaaac gacccgggaa gattttcgca
    16081 aagtcggcaa acaacgattg caccatcaga ttagagtgca gttccgatac accgttaact
    16141 ttgtggctca caacaaccgc cagccaggcc atacgcacac gacgaccgtt ggattcatca
    16201 atgatcgacg cccgtcccag cagatcggta tcgttcggat actgttcctg caaggttttc
    16261 aggaaatagt cgttgatttc aaagatgatc tgcaggtgac gcggcagaat tttacccagc
    16321 atatcaaccg gccaggtttc cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag
    16381 acctgacaac acacctcaaa cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga
    16441 cgcatcatct caggaatcga cagtaccgga tgggtatcat tgagatgaat cgcgatttta
    16501 tccgccaggt tatcgtaggt tttatgcaac tgataatggc ggcttaaaat gtcctgaatg
    16561 gtcgaggaaa ccaggaagta ttcctgacgc aggcgcagct cacgcccgga gtaggtggag
    16621 tcatccggat acagtacgcg agatacgttc tcggagtggt ttttatcttc cactgccgcg
    16681 aagtagtcac cctggttgaa tttaccgagg ttaatttcgc tactggcttg cgcactccac
    16741 aaacgcaacg tgttggtcgc gtcggtgtcg taccccggga ttatctgatc gtaagcgact
    16801 cccagaatct cttcggtttc aatccagcgc gtttttttac cttcctgctg aatgcgacca
    16861 ccaaaacgga ctttatagcg cgtgttgtgg cgtttgaatt cccacgggtt accatattcc
    16921 agccagtagt caggcgactc tttctggcta ccgttaacga tgttctgctt gaacataccg
    16981 tagtcatagc ggatgccgta accgcgcccc ggcaacccta acgtcgccag agaatcgagg
    17041 aagcaagccg ccagacgtcc caggccaccg ttaccgaggc ccgggtcatt ttcttcatca
    17101 atcagctctt cgagatttaa ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt
    17161 cctagcgaca acatggcgtt ggagagcgta cggccaatca aaaactccat cgacaagtag
    17221 taaacctgac gagtttcttg cgacaactgg gcacggtttg aacgtaacca gcgctccacg
    17281 agacgatcgc gcacagcaaa taacgttgcg ttcagccatt catgtttatt ggcgacgacc
    17341 gggtcctttc caatcgtaaa catcagcttg taagcgatag agtgcttaag agcttctacg
    17401 ctaagcgtgg gcgatgaata tgtaaacgga gcattcatat aggcgtttcc tgaaaactat
    17461 ttcaagcgat agtaaagctc acggtacgac ttcgccgcga cctgccagct aaaatccatt
    17521 gccatagcct gacgttgcac aaaccgccac agtgaaggac gggaccacag tacaaaagca
    17581 cgtcgaatag cccgtaatag cgaccaggca ttactatctt caaagacaaa cccactggcg
    17641 acgccatctg caaggttctc aagagaacag tcagaaaccg tatcagcaag cccaccggtg
    17701 cgccgcacta acggcagcgt accgtacttc aatccataaa gttgcgttaa gccgcacggt
    17761 tcaaaacggc tgggtaccag aatgacgtcc gcgccaccca taatgcgatg cgaaaatgct
    17821 tcgtgatagc caatctgaac gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa
    17881 ccttcctgca gcaccggatc gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga
    17941 aggcccggta aggcttccag caccaggtcg agacctttct ggctggtcag acggctcacc
    18001 actgcaaaaa gcggcacttt atcgtcaacc ttaagcccca ttgcgatttg taactggcgc
    18061 ttattttccg ctttatcttc caacgtatcg cgtgtgtaac gcgaggccaa cagtaagtcc
    18121 gtctctggac tccagatttt ctcgtccacg ccgttcagta cgccggaaag acgcccttca
    18181 cggtgacgct gttgcaacag accttccata ccgtaggcaa actgcggttc ggtgatctcg
    18241 cgagcgtagg ttggactgac cgccgtaatg tgatcggcat agtacagacc ggccttcagg
    18301 aaagagattt gtccgttgaa ttccagccca tgaatattaa agaatgacca tggcaattgg
    18361 atgtcattca tgtgatgtgc ataaaacatg ccttgatagg ccaggttgtg cacagtaaac
    18421 accgacttcg ccggacgccc gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag
    18481 tcgtgcgcat gcaccacatc aggacgccag aatgggtcaa gcccgttggc catttctgcc
    18541 ccaacccacc ccagcagcgc aaaacgcaat acgttgtcgg tataggcaaa taagttggta
    18601 tcgtgatacg ggcttcccgg acgatcatag agatgcggcg cgtcaatcag gtatatgcca
    18661 accccgttgt aatgaccgaa caacagcgtg atatgcccgg cgaaggtatc acgacgggat
    18721 actacctgcg catcggtcac gccacggcga atatcgggaa atgcaggcaa cagtacgcga
    18781 gcgtcaacgc cgtctgcgat ttgtgctgcg ggtaatgccc caataacatc agccagaccg
    18841 ccggttttaa gcagcgggaa catctctgaa catacatgta aaacctgcat tatcgctcct
    18901 gtttatgccc taacttccgt agcatttcgc gcgttaccag cacgatgcct tcttctgaac
    18961 gatagaaacg acgtgcatct tcctctgcgt tttcaccaat caccatgcct tccggaataa
    19021 cacaagcacg atcaatgacg cagcggcgca gacggcacga gcgacctacc catacttccg
    19081 gtaacaatac ggcggaatca atgttgcaga atgaattcac gcgaacgcgc gagaacagaa
    19141 cggactgcac caccaccgaa ccggagatca cacaaccgcc ggaaaccagt gagttaaggg
    19201 tcatcccgtg gctaccggag cgatcctgca cgaatttcgc tggcggtaat gattcattgt
    19261 aggtgcgaat tggccaattg cgatcgtaca tatccagttc cggcaccaca gaggccagat
    19321 cgaggttcgc tttccagtaa gcttccagcg tacccacatc gcgccagtac ggctcggcat
    19381 ccgggtcgga ttgtacgcaa gagagcggga acgggtgcgc ataggccaga ccggcttcgg
    19441 tgatcttggg aatcaaatct ttgccaaagt cgtggctgga gttctcatcg cgatcgtctt
    19501 cttccagcag ttcatacaga tagtcggcgt caaagacgta gatacccata ctcgccagag
    19561 atttgctcgg atcgttcggc attgacggcg ggttagcagg tttttcaacg aattcgataa
    19621 ttttatcgtt ctcatcaacc gccataacgc caaatgcgga ggcttcttca atcggtactg
    19681 gcatacaagc aacggtgcaa cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt
    19741 agtcttgctt gtagatatgg tcgcccgcca ggatcaccac gtattccgct ttataacggc
    19801 ggataatatc gagattttgg gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt
    19861 tcattctctg ctgtgctggc agcagatcga caaactcgtt catttcttca ttgaagaatg
    19921 accagccgcg ctgaatgtgc tgcaccagag tgtgggactg gtactgggtg atcacgccca
    19981 tacgacggat cccggagttg atgcagttag acagcgcaaa gtcgataatg cggaacttac
    20041 cgccgaagtg tacggccggt tttgctcgct tattggttaa atccttcagg cgggtaccac
    20101 gtcctcccgc cagtatcagg gcaacagatt tcaatggcag ctggcgcgcc aacattaagt
    20161 gatcgttctt ctctaaacta accatgacta actccttttt tatcatctct ggaacacaca
    20221 caatccgtgt gcaggtccct gccagacagc cgtaatcact gggttatcct ctccagcgaa
    20281 tgggggaatg gcgtgccact ccccagcagg taaaacaatc tctgttacct caagcgtggc
    20341 gttaattgcg atcaaaaagc gatccgagag cagaatttgc agctgtttcg gcccgttttg
    20401 ccactcatcc gtgcttaaag gttgagcata tcgatttagc caacggacat tgccgtcgcc
    20461 ttcttcccac cagcgattct ccaccaaagc gggaatacgc ttgcgcagat ggattaacgc
    20521 ggcggtaaat gcggttaaac cactgcttgc ctgcgaccag tccaaccagg ttaattggtt
    20581 atcctgacag taggcattgt tattgccatg ctggctgtga ccatgttcgt caccggccag
    20641 taacatcggc gtgccctggg agagcaacaa cgttgttaac agggcatgaa tgctgtcgcg
    20701 ccgccgttca accagatcaa gagtaccgcc taacccttct ttaccatgat tgttactgta
    20761 attgttgttg gtcccgtcgc gattttcttc tccgtttgct tcattgtgtt tatggttgaa
    20821 gcaaacgcag tcgcgaagcg taaaaccgtc atgtgcggtg acgagattaa tcgcggcact
    20881 cggcagacga ccattacgtt taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc
    20941 ccccagaggc aaatcataat gcagccagaa acgacgggca gcatcgcgga aatgatcgtt
    21001 ccactcggca aaaagcggcg ggaaatttcc cacctgataa ccaccaggag cgatatccca
    21061 cggttcagca attaacttca cctgcgagag caccgggcag ttctggatag cggtaaacaa
    21121 cggcgcatcc tgacggaact ctggcgtacg gcccatgact gccgccagat caaagcggaa
    21181 accatcgacg tggcaggttt ctacccaata acgcaggcag gcgctggcat aatccaccac
    21241 cgccgggtga ctcaaattga gcgtgttgcc acaaccggtc cagttgtgat aatcgccgtc
    21301 ttctcttatc caataatagc tacggttgtc gatcccacgc agcgagaata acgggccgtc
    21361 gaggtccagt tccgcactat ggttgagcac gatatcaaga atgacttcga tacccgcttt
    21421 atgcagtgct ttgattgcat cgcgaaactc atccagcgcc gtttctggcg agcaggcata
    21481 cgccggatgc agcgcaaaca tcgccaccgg gttgtaaccc cagtagttac ttagtcccat
    21541 gcgttgcaga cgtggttcac tggcaaactg cgccaccggc agcagttcca gcgcggtaat
    21601 gcccaactgt ttcaaatagt tgatcatcac cggatgcccg agggctttat aagtgccacg
    21661 gatctcgacc gggatctccg ggtgcaggta cgttaatcct ttgacatggg cttcataaat
    21721 gatggtgctg ccccacggcg tgcgcggcgg agcatcatct tcccagtcat agtgatcaac
    21781 caccactacg catttcggcg caatggcggc gttgtcgcga tagtcaggtt cattatgacc
    21841 ggcgtgcagc agcgggttat ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat
    21901 caacaacttc gccgggttaa agcgatgccc ctcggcgggt tgccaggggc catgaacgcg
    21961 ataaccataa cgcaaacccg ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact
    22021 gtgccctggc aagtcatagc gatgttcctg gccattggcg tcaaagacac acagctctac
    22081 ccgctcggca tgagcggaga aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc
    22141 gaggggagcg ggtttgccaa tggcgagttg tgtcattctg cctcccgaac cagccagata
    22201 gtggccagcg gtggtagcgt caggcttagt gaatgctgac gaccgtggct ggcaatctca
    22261 tcgctgtgta ccgtgccgcc attgcctgca ttactgccgt gatagtgcat ggaatcggta
    22321 ttgaggatct cacgccattt gcctggctga ttaatgccga agcgataatc atgacgcggc
    22381 accggcgtaa agttactggc aacgatgatt tcgttaccct ctttatcgcg acgcacaaag
    22441 atcagcaccg agcgttcttt gtcatccacc accagccatt caaagccgta cgggtcaaaa
    22501 tccagttcat gcatcgcttt atggtggcgg taggtgaggt tcagatcgcg caccagacgc
    22561 tggacaccgt ggtgccagtt atcgccgcct tccaacagat gccagtcgag gctggcgtca
    22621 tggttccact cgcggccctg ggcaaattcg ttccccatga acagcagttt cttgcccgga
    22681 aatgcccaca tccagccata gtaagcgcgc aggttagcga atttctgcca tgcgtcgccc
    22741 ggcatgcggt cgagaatcga ttttttaccg tggaccactt catcatgcga caacggcagg
    22801 acgaagtttt cagtgtagtt gtagagcatc ccgaaggtca gtttatcgtg atgatactga
    22861 cgataaatcg ggtcgagctt catgtagtcc agggtgtcat gcatccagcc gaggttccac
    22921 ttgtaccaga agcccagacc gcccatatcc tgcggacgag aaacgccagg gaaatcggta
    22981 gactcctcag ccattgtcac cgcaccggaa acctgctcac caagaatacg gttggtatta
    23041 cgcaagaatt caatcgcttc aagattctcg cgcccgccaa attcgttcgg gatccactcc
    23101 ccctctttac ggctgtagtc acgataaatc atcgacgcca ccgcatcgac gcgcagcgca
    23161 tcaataccaa aacgttcaat ccagtaaagc gcgttaccga cgaggaagtt actgacttca
    23221 cggcgaccat agttgtaaat cagcgtgttc cagtcctgat gatagccttc gcgcggatcg
    23281 ctgtgttcat acaagttcgt accatcaaat tcggcaagcg caaagtcatc agtcgggaag
    23341 tggcctggca cccagtcgag aatcacgttc agaccagctg cgtgtgcagc atcaatgaaa
    23401 taacggaagt cgtcgcgagt accaaaacgg cgggtcggtg catacaggcc ggttggctga
    23461 taaccccaac tgccatcgaa gggatgctcg ttaatgggca gtagttcgag gtgggtaaag
    23521 cccatccatt tagcataagg caccagttga tcggccagct cgcggtagct caaccagaaa
    23581 ttgttgtcgg tgtggcgacg ccaggagccc aggtgaactt cataaataga gattggcgca
    23641 tcaaactgat tcgctttttt gcgctcttca gtctgcacaa ccttttccgg tagcccgcaa
    23701 atgagagacg cggtttccgg gcgcatttgc gcttcgaagg cataagggtc ggacttcaga
    23761 cgcaagttgc cattggcatc aatcatctcg tatttataga gctgaccgtt atgcgcccca
    23821 gggataaaca gttcccaaat gccgctctct ttacgcagac gcatcgggtg acggcgaccg
    23881 tcccagtagt tgaattgccc aaccaccgag acccgacggg cgtttggagc ccagacagag
    23941 aaacgcgtac ctgtgacgcc atccatagta tctgcatgcg cgcctaaggt ttcatacggg
    24001 cgcaggtgag taccttcaga taatagccag gcatccattt cctggattag cggaccaaaa
    24061 cggtaaggat catcaatcag gttttgctga ccatgccaga caacagccaa ctgatagcgg
    24121 aaaaaattct tacgtcgcgg aatgacgccg ctaaagaatc cccgtgagtc gagacactcc
    24181 agttttgcga gtttgcgccc ggttttcggt tcaatcaccc acacatcggt agcgtcgggt
    24241 aaaagggcac ggacttccag tcccgcggtg gttttatgca ttcccagtac ggaaaaagga
    24301 tccgcaaaat ggcctgcaat tagcgcgtta atcacgtctc tatcgatacg atcggacatg
    24361 cttgtcttcc tgttttattg tgtcacccca tccaattgga tttttgactt ctggttgtga
    24421 catttttttg acctgaacgg cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc
    24481 aggtaaggct gtgaatactc atgtattccg ccacccttaa agaatagcca atgctctatt
    24541 taactcccgg taaatcatga aacatctgcg cttactcctg tattacgcac taacaggggc
    24601 ggcatcgcgc cccagattta atgaataaag attacgccag ttgacgaagc atccgacgca
    24661 gcggctccgc ggccccccac agcagctggt cgcccacggt aaaggctgac aggaactctg
    24721 gtcccatatt cagcttacgc aggcggccta ccggcgtggt cagcgtgccg gtaacggcag
    24781 ctggggttag ctcacgcata gtgatttccc gatcgttcgg aacgactttc gcccacggat
    24841 tgtgcgcagc cagcagttct tccacggtcg gaatcgacac atcttttttc aatttaatag
    24901 tgaatgcctg gctgtggcag cgcaatgccc cgacacgcac acataaacca tctaccggaa
    24961 ttacggaaga tgtgttgagg atcttgttgg tttccgcctg ccctttccac tcttcgcggc
    25021 tctgaccgtt atcgagctgt ttgtcgatcc acggaatcag gctacccgcc agcggcacgc
    25081 caaagttatc caccggcagc tcaccgctac gggttaaggt tgtgactttg cgttcgatat
    25141 cgagaatagc agaggacggg gtcgcgagtt catctgctac atggccatac agatggccca
    25201 tctgggttaa taactcacgc atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg
    25261 caacggacac ccaatcaaca agatcattgg cgaataaacc acccaacgac atcaacatca
    25321 ggcttacggt acagttaccg ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa
    25381 tgacgtcctg attgacgggg tcaagaatga tgatggcgtc atctttcatg cgcagagacg
    25441 atgctgcgtc aatccagtaa ccctgccatc cgctttcacg aagctttgga tagatttcgt
    25501 tggtataatc gccgccctga caggtcacaa tgatatcgag ggcctttagc gcctccagat
    25561 caaaggcatc ctgaagtgtg ccagtggttc cgccaaaaga cggcgcagcc tgaccaagct
    25621 gagaagtaga aaagaagaca gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt
    25681 gcatgagaac ggagccgacc ataccgcgcc agccgataaa accaacattt ttcataagcg
    25741 ttttttcctg caaagatgtg tgctgtataa atgtgccggt ctcctcttgg cacatctttc
    25801 accatacaaa aagcagccaa agtcgcaagt gaaattaatc aatgatagcg aagccatcag
    25861 taatgcgact tatcctgctt tgttagcacg cagaaagtcc gcggcaatta tcagggaatt
    25921 tgagttatga atgaaatcat ttctgcagca gttttattga tcctgattat ggatccgctc
    25981 ggaaacctac ctattttcat gtccgtactg aaacatactg aaccgaaaag acggcgggca
    26041 atcatggtgc gagagttgct tattgctctc ctggtgatgc tggtgttcct gtttgcgggt
    26101 gagaaaattc tggcatttct cagcctgcga gcagaaaccg tctccatttc tggcggcatc
    26161 attctgtttc taattgccat taagatgatt ttccccagcg cttcaggaaa tagcagcggg
    26221 cttccggcag gtgaagagcc atttatcgtg ccgctggcaa ttccgctggt cgccgggcca
    26281 actattctcg ccacgctgat gttgttgtct catcagtacc cgaatcagat gggacatctg
    26341 gtgattgctc tgctgctggc ctggggcggc acctttgtca tcctgctaca gtcttcgcta
    26401 tttttacgtc tgctgggcga gaaaggggtg aacgcacttg aacgcctgat gggattgatt
    26461 ctggtgatga tggcaaccca gatgttcctc gacggcattc gaatgtggat gaaggggtaa
    26521 ggttcaaaca ggtgccgcac acaaccagac gcggcacctg ttttattacc tgaactgata
    26581 atgaccttcg cccaatcgga taacccttcc ctcttcttct gcgcggttca gccacacact
    26641 aatttgttcg atggttaaac cagtacaaga cgccagcctt tcaatactta tcgattcctt
    26701 tgcaaggatc gccagttccg ccacaaagag ttgataaaaa tcgacaggcg tatacgcggg
    26761 cattactgcg taatttgcgt catcttcatg tagcgaaaac agttctggtt gagcgttacg
    26821 gagtgctctg tcatacatca gcccaggaac gtctggcagt ctgatcgtca cattctcagc
    26881 ctgttcagca ctccatgacg ccccattttc tactaattgt gaattggctg attgcttatc
    26941 ctgatttggt ttaacctgca caggcagcca ctgatgtttt aatgcctcca tcgccccggt
    27001 tatcgttcca ccggtcattc ccgcacgtac aaccattgcg ctttcagcaa ggcaataaat
    27061 atatttattt cgcgccatcg cattgccgac ggtgaaacgt acctctgggt aaaacggcga
    27121 aatcaacacc aggttgcctg ctataagccc ttcacgccat ttcactaacg tactcgtttt
    27181 taacaagcta tcagctaata cgccaacggc agttcccccg gcctccagtg ccgacgccat
    27241 tgcacattca tcaatacctc gcgcaccacc agagataacg caaatcccct gttgggccag
    27301 tttcgcggcc agttgttggg tatagcgcaa atcgtcagtc ggagcatctc gcgagccaac
    27361 aatcgccata ccttccgctt gcagtaatgc tttattcccg cagccaaata aaacgggagg
    27421 tgcatccgtt cgcaatcggt ttttcaagcg aacgggataa tcagcatctc cgcgggttaa
    27481 gatccataaa ccggcgcgtt gccacttatc taccgccaac gccagactat gaccacgcgc
    27541 cagcaagcca agaatacgct cctgagaaat acgcggatcg ctccagcctg taagtttctc
    27601 ttgcggttgc ggcaccagaa gctcggcggg actgatacgt tggtgcttca gccataatgc
    27661 aaacttcccc cattcactat tactaagagg tttatactca ctcgccgccg cgcgagaaaa
    27721 atcgctggtt agcaggagag ttgcttgtgc attggctgaa agattcatca attttttacc
    27781 gaggtagaag caagggcaag aggataaacc gtcgggcaac ctgcctggcg taacagtgct
    27841 gtcagaactg tcagcgtcca tgcagaatct acgatatcgt caaccagcaa cgccggacca
    27901 ggcatcaaag gaggggtaat aacaaacgcc ccgtcgagat tttgacactg gtggaaacgg
    27961 ttttgctgca ttttctgcgg tggattgtcc acgacttttt caatggcatc aataaaaggt
    28021 aagccaagtt tcgccgccag tcgccgggca aaatcaggaa ccaggtcgag gtgctttaat
    28081 gaaggaacgc agcataccca ggttggttca ggatgcggat tccagcgttg gcgaaccatt
    28141 tccgcacatg cctccaccag ttcatcactg aatcgccccg tttttttctc ctgtgctacc
    28201 tgctttcccc acccggaatc tccccaacga gaaaggattc gccccgtaga tccttgtaaa
    28261 ctcgcaggca agttcccttt aaatccgtat tgcgtaaaag ccccagctgc cacctgttta
    28321 ttgagattta atggcaggtc agcgtgttta ataaataagt ttgccgcatg gagtaagccg
    28381 ctatcaatgt cgggcgacaa caacggatgc tgaagacaac tgctacattt accacaatgt
    28441 tcggcacctg gctcatcgag tgcgcggcgc agaaattgca ttttgcactc cttggtcgtg
    28501 atatagcttt caacgtctgc cagctcactt tttctctgat tcatcaaatg gttaattcgt
    28561 tcatggtcag gagaaaaact gacaattgtt ctgcgccata atttctcggt atacaccaca
    28621 ggcgatggat tttcagctac caataatttc agtgcttttt ctatttgccc ataacggaga
    28681 ttcgtccgtt gttcaatgcc tcgtaaggta agaccgtcat tcacgctaag tacgttgagt
    28741 atttcatgaa tttgcgcctc cgcaggaaag gcgctttcac ggaagaattt atgaatagca
    28801 cggtcttcac tgccgcaaag cagtatgcca acagcagaat cgataccgcg accggcacgc
    28861 cccacctgtt ggtagtaacc aactatcgaa cctggcatct ggtaatgaat aacaaacccc
    28921 aaatccggtt tgtcaaagcc cattcccagc gccgttgtcg caaccagcac tttaatttta
    28981 tttgccagca acgcctgctc cagatattcc ctggcagtat tagaatcttc cgcgccttca
    29041 caagtcacgc cgctgtagta agcaaatgca ctgataccat ttttccttaa ccactcggcg
    29101 acaagttcgg catcgcgagt agtcagggta taaacaattc ccgatttgga aaactgaggg
    29161 ataaccgttg ctaaccatgc cagacgggat gactgttcac ctaagactaa ggcatcaagc
    29221 gccaaacttt cgcgagccag cgttccacgc tgaatcacaa tgtcacccaa ttgctgacgg
    29281 atatcctcaa cgacacggtt attcgctgtc gcggttgtac ccagaacagg ggtattcgca
    29341 ggtagttggc gcaaaatatc taatatacgt cggtaatccg ggcgaaaatc gtggccccaa
    29401 tcagagatgc agtgcgcctc gtcgaccaca agcaagccaa tacgatcggc tataggatat
    29461 aaaaccgttt cgataaagtc ctggtttgcc aaacgttccg gggaaaccag caagcaatcg
    29521 acctcacctt gcagcaactt atcgctaatg cgctgccatt cttctctgtt tgtagaattc
    29581 aacgtttcag ccgtaatacc taaccgttca gcggcggcaa cctggttacg catcagggca
    29641 agcaaaggag agatgataat tgtcgggcca gcgccgcgat cgcggaagat tttactggcg
    29701 atgaagtaca cagcactttt cccccagcca gtgcgttgga caaccagcag ttttcgccgt
    29761 tggttcacca ccgcgtctat ggcttcccat tgcccttccc tgaaagtagc ggtggtattt
    29821 cctaacatcc gctgtaaaag caattcagct gcgtgctttt ccatctttat ctcctgcgat
    29881 ctttaggcag tgattagaac ggattatttg caggtaaatt tgcgagagag atcaaaaaga
    29941 tgaagagagg agaaaagaga aatgaattac tgcgagcaag ctcgcagtaa tttgtcgcag
    30001 taattaactc aacagctgga acgcaatcat cccaacgatg gcaccgacag tgccgaggat
    30061 ggtttccatc atggtccagg ttttcagcgt ttcggcttcg gtcgcgccgg taaatttacc
    30121 gaacaaccag aaaccagcgt cgttaacgtg gctgacaaca atcgaaccac cggcgataca
    30181 aatcgacagc gccgccattt gcgcaccgga gtagttcagt tgttcaataa ccggcatcac
    30241 cagtcccacc gccgttaaac aggcaacggt tgcagaaccc tgaatgatgc gcactgcagc
    30301 ggccagcacg aagcaggtga tggcaatcgg caggcccatg ccggttaacg cttcacccag
    30361 cgccggacct acgccagagt caaccagcac ctgtttgaac acgccgcctg caccaatcac
    30421 cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa atctccatca ctttgtcttt
    30481 tggcatgccc tgacgcattg ccaggccata aatcgctacc agacaagcaa ccagaatcgc
    30541 ggtaaacgga tgaccaataa actcgaacca ttcgtaagcg gtagagcctt ccggcacaaa
    30601 acgcgcggca atggttttca gccccaccag caccagcggc agcaggatca gcgacaggct
    30661 gaatccgaag gacggcattt tgccttcgcc gagatgcggg tcgctgatgt cgtcaggaat
    30721 atgcagctca acgtaacggc tgatgaagtt accccacagc ggcccggcaa taatcattcc
    30781 cggaattgcc gcacacaggc caatcaggat catccagcca aaatcggcgt tcatctgcga
    30841 tgccagcagc attggcgctg gccctggcac caggaacgcc gccgctgccg ccacgcctgc
    30901 gaataatggg attaccagct tcaccaggtt cgtaccggtg tggcgcgcca ttgagaaagc
    30961 aacgctaatc agcagaacaa tcgccacttc aaagaacagc ggtagcgcac agaccagccc
    31021 cgcaaggccg atggcataat gcgcgcggct gtgaccgaag gatttaagca ttttgacggc
    31081 aatctgatcg actgcgccgg tttcatgtaa gatcttgcca aacatagctc ccagggcgac
    31141 aaccaccgcc aggaagccga gggtgcctcc catccctttt tccatcgtcg ctgcgatttt
    31201 atcgagcggc ataccggaaa aaaggccagc ccccatggac accaccatta aagccaggaa
    31261 agcgtgcata cgcgccttca tgactaaaaa cagcagcagt aaaacagacc ctactgctgt
    31321 taaaacaagc gttaatgtag tcactactta tttgcctttt ttaataacct caatggtgct
    31381 tgccacaaca ccttccagcg gttgatcgat atccaccacc agtacatcgg tttcgtccgc
    31441 acccggctcc tgcagcgttt caaactgcgt caccaacatt tgggttttaa agaaatggcc
    31501 tttgcgcgct ttcaggcggc tttcaatcac atcaaaatcg cctttcaggt agatgaaaga
    31561 gagattcgga ttaccttcac gcagcaagtc gcgatagtgt tttttcaatg cagaacagac
    31621 gatcagcgac actttattag tgcgctgcat agcaaacgcg gcgtcgttca gcgcctgcaa
    31681 ccacggtttg cgatcgtcgt cattcagcgg ttcgccagac gccatttttt cgatattgcg
    31741 ccgtggatgg aggaaatcgc catcaagaaa cgcggcatga agttgatgcg ccacttcact
    31801 tgcgaccgca gatttgccgc tgcccgatac gcccatcaag acgtaaatgt ggtgatcatg
    31861 gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat taaaaatgtt acgggtaact
    31921 gttatcggta acattctccg gccggacaat atcagaagca atatccatcc gtgccttaag
    31981 tgtataagtg tgagctactt caaatttgtg ggcttaaata gatccgcccg gtgacaaggt
    32041 gaaacctaaa tctaacattt taggtgtcac agattcgcca cgaatacgcg ccagaaggcg
    32101 ttcagcgcca atactgccca tccgctcacg cggcgtcagc acgctcgcaa gtcgtggctc
    32161 catcacctga ccaatgtcat gaccgtggaa accggcaatc gccatatcgt caggaacttt
    32221 taaccccaga cgctgacatt caaacgccgc gccgaccgcc agatcatcat tagtacagaa
    32281 cacgccatcc agctgcggat attcccgccg cgcctggcga atcagttcaa taccggaaga
    32341 gtaagaagaa gattgctcaa ccatcacgct atacggcacc aggcctgcat cccgcatcgc
    32401 ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg agacgtgcgc cgagataggc
    32461 aatatggcga tgcccgcgag caataatggc ggcggtcatc tggcgtgccg cttcaaagtt
    32521 atcaaaaccg acggcaatat cgaggcatgg cgacttgctg tccatcagtt ccaccacggg
    32581 aatccccgcc acttcaatca tctttaaggt gcgcggcgtg tgggtacgtt cggtgaggat
    32641 aagtccgtcg atattccatg agagcattga ttcgagacgt tcttgctcca tttccggttt
    32701 ataaccgtag tgggccagca tggtctgata accgtgcgcg tcggtgacgc tttcgattcc
    32761 gcgtaatact tccgcgaaaa cctggttggt gagagaaggt aacaggacgc caatcgcccg
    32821 gctggtggcg ttagagagga tatcgggcgc gcgattggga atatagccca gttcatcaag
    32881 agccgcggca atcttgccgc gtagagcgac ggaaacctgc tccgggttgc gtaaaaaacg
    32941 gctgaccgtc attttggtca cgcctacacg gtcagccaca tcctgaagta cgggtctttt
    33001 ctttttcatc gtcctgaagg tacaaaagag atagattctc ttagtttaac acggacgcat
    33061 cagaaccttc ccggggaaaa cagtgcttgt ttacaattat tagatataca ctaaatcaat
    33121 tgcatcgcat tgtgctaacg acgcggcaaa accccggaag cgtacatcag cacgcgaccg
    33181 gagcgagtga ggaagcctgc aaaagggcag gcttcaaaga tgccgttgag ttttaaaccg
    33241 gcggcagatc gaacagtaac acttcgctgt cgctatccgc atggatggag attgcctgct
    33301 catcccagat tgccagacca tcgctggtcg aggctttcac gccgttaatg gtgacattgc
    33361 ctttcaccac ctggatccag acgcggcgtt cagcggcaat ctgatgcacc gactgctcat
    33421 ctttcagcaa cgcccagcgg tacagttcca tatcctgatg cactttcaat gaaccatcac
    33481 gcgcatctgg cgaaagcacc agttgtttgc cctgcactgc atcgaagcga cgctgttcat
    33541 aacgcggcgt aataccgttt tcttcaggca tgatccagat ctgatacaga tgcagacgct
    33601 cggtgctgct tgggttgtac tctgagtgac gaatacccgt accagcactc ataatctgga
    33661 actcgcccgc cggaacctgc tctttattgc ccatgctgtc ctggtgctca acagtacctt
    33721 ccagcacgta ggtcaaaatt tccatatctt tatgcgggtg agtgccgaag ccctgccctg
    33781 cttcaatcac gtcgtcgtta atcacgcgca gcgcggagaa gcccataaag ttcggatcgt
    33841 aatagttggc aaaagagaaa gtatgccagg agtccagcca gccatgattt gcatgaccac
    33901 gttcatttgc tttgcgtaag tagatcattt gtattcgccc cctgaatgat ttcgatgggc
    33961 ttagtgtgga cccgatctgc ctgggatgat agagggtgaa aattgacccc tctgttcaaa
    34021 aaaattgaac tattcgaggg gccatcaaac ttacttcacg agagtcacgg tggagggaga
    34081 ggcttgctcg aatccgcgtt caaggatttc cagattcgta agaacttcag attccttgac
    34141 gtaatttggc gcaccgtggg tgatggtttg atacaacgca tcataaacgc gcccgtaatc
    34201 gcccatctcc ggcttcatct cttctctgac cgtcacgccc tcgtcattga catactccag
    34261 cacaccgacc gaatcatccg ctgcgaatcc cggttcgccc ggcataatat tagccttcag
    34321 gctggtttcc tgctggtcga taccgtattt aataaacgaa cctttcttac cgtgaacgat
    34381 aaatttcgga taatcgattt tcaccagatg gctggttttg acgatggctt ttagatcgcc
    34441 ataaaacagt tgcgcttcga aagtatcgtc aggattggct ttattgcgca ggctgcggat
    34501 gtcataagcg acgtgatccg ggcgaccgaa cagagaaata atctggtcca tcgtatgcac
    34561 accaaggcca tagaacgcgc catcctgcgg cagcccaggt ttggtttctg ccaccgggcg
    34621 gtaatagtca aaatggcttt ccacttcgac aatctctccc agcttgccgc tttcaatcgc
    34681 ttttttcgcc gtcaggaagc aggaatcaaa gcgacgattc tgatatggcg tgacggtcag
    34741 ccctttgctt ttcgccagtg caaacagctc tttcgcctgc gcaagtgtcg gagtgaacgg
    34801 tttttcgacc agcacatttt tcccggcttc cagcgcgcgc ttcgcgtact cgaagtggct
    34861 gtccgcatgg gtgcagacaa caaccagctt aacatcggga tcgtttagca cttcgttgag
    34921 atcgctggtg aaatggatat gagagtaaat gggagcttgt tcttccggct ttgcatggcg
    34981 gcgaaaaata tgcgcgacat gccagctatc cttgcggtta agtacatacg gcagatggta
    35041 acgggtggtg cttttgccga agccaataaa ggcgcagttg atgaccatga tttcgtcctt
    35101 tttaaggtgg ttatttacac cttagcgcaa agcggacgtg gttcctactg gagtgcgcat
    35161 aacgaacaca agcacttccc gaggataaat tgagaacgaa agatcaaaaa aaaagccagc
    35221 acccggctgg ctaagtaata ctggaagcaa tgtgagcaat gtcgtgcttt caggttctcc
    35281 gcgagggtct tcctgatcgc gagacaataa taatcattct cattcgcact tgtccaacac
    35341 tttttgcaaa aaaatgcatt tgactcgcat tcgaaagtca atgatgttga aagggacatt
    35401 taccccaaag aggacaaagg aatgagtgag atagtgatac gccatgctga aacgcgcgat
    35461 tacgaggcca tcaggcagat tcacgcccag ccggaggtgt attgcaacac actacaggtg
    35521 cctcatcctt ccgatcatat gtggcaggag cgactcgccg atcgtcctgg catcaaacag
    35581 ttagttgcct gtattgatgg aaccgtcgtg ggtcacctca ccattgacgt gcaacagcgc
    35641 ccacgccgca gtcatgttgc cgattttggt atctgtgtcg acgcccgctg gaagaatcgc
    35701 ggcgtcgcca gcgccctgat gcgagagatg attgaaatgt gcgacaactg gttgcgggta
    35761 gatcgtattg agctaacggt gttcgtcgat aacgcgccag caattaaggt ctataaaaaa
    35821 tacggctttg aaattgaagg gactggtaag aagtacgcat tgcgtaatgg tgaatatgtt
    35881 gatgcgtatt ttatggcgcg ggtgaagtaa agaatattct caaattgcct gttaccaatg
    35941 tgaataatta gttttgttca tctaatttga gtttttcctt atattaccac catcattttg
    36001 aaacaatttg taacatcaag agacttttga atcattactc gttaatattt cttattaaag
    36061 agaacatatt ttcgcaagca atagttatta ctgtttgttt atggatagag agtctactta
    36121 atgggtacag cagctataag tcatttacga tatgatctta acaaatatgc cctttcttta
    36181 cgcaaaaccg caacacttgc ttcaacattt tttattgagt ccccattagt tagattcgag
    36241 tatctgcaag aaatagaaaa tactattaat gatattactc acagattcaa ttcttcatat
    36301 gacatcaatg aaaaagcacg acttatcaat gaattaaaaa tggagtcaga aacagcaaga
    36361 aaagaatatc aacttttcag acaaggaaat tatgataaat atatcactac tgatattttc
    36421 gaagaacatg gtttgataaa atatgtgaac ttaagtttcg atattgttgc atctgtagga
    36481 caggtcgtag gcggggttgg cgcgttgaaa tttggtaagg tagtgcattc aaaccgaata
    36541 aaaggtatag gcgtcacatt agttgctcat ggggcgaata atttttatga aagcttatca
    36601 cctttatttt ttaacgagta tgattcaggt ccaatacgtg aactctacag agtaatagca
    36661 aaaaaaatgg gaggtgatat taattcttct gattatgcat acagcatcgt ggacttttcg
    36721 attactgcat atggtggata ttcaggcatg aaaatcgtac ctaagtataa tagattgatt
    36781 cgt
//
LOCUS       DAPGRN010000040        36203 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPGRN010000040 DAPGRN010000000
VERSION     DAPGRN010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.040"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RHW33_002133"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="RHW33_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV1645633.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RHW33_002134"
     CDS             complement(2817..3548)
                     /gene="pgeF"
                     /locus_tag="RHW33_002134"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV1645634.1"
                     /translation="MSKLIVPQWPLTKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEATVACFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RHW33_002135"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="RHW33_002135"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV1645635.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="RHW33_002136"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="RHW33_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV1645636.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="RHW33_002137"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="RHW33_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV1645637.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="RHW33_002138"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="RHW33_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV1645638.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="RHW33_002139"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="RHW33_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV1645639.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="RHW33_002140"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="RHW33_002140"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV1645640.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="RHW33_002141"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="RHW33_002141"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV1645641.1"
                     /translation="MQKDALNNVHITDEQVLMTPAQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..10239
                     /locus_tag="RHW33_002142"
     CDS             9874..10239
                     /locus_tag="RHW33_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV1645642.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYTRGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASAIRLN"
     gene            10389..10907
                     /locus_tag="RHW33_002143"
     CDS             10389..10907
                     /locus_tag="RHW33_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV1645643.1"
                     /translation="MRFSHRLFLLLIILLTGAPILAQEPSDVAKNVRMMVSGIISYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISDCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10897..12131
                     /gene="dgcN"
                     /locus_tag="RHW33_002144"
                     /pseudo
     CDS             10897..12131
                     /gene="dgcN"
                     /locus_tag="RHW33_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            12147..12629
                     /locus_tag="RHW33_002145"
     CDS             12147..12629
                     /locus_tag="RHW33_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV1645644.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAKGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12706..13053)
                     /gene="rplS"
                     /locus_tag="RHW33_002146"
     CDS             complement(12706..13053)
                     /gene="rplS"
                     /locus_tag="RHW33_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV1645645.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13095..13862)
                     /gene="trmD"
                     /locus_tag="RHW33_002147"
     CDS             complement(13095..13862)
                     /gene="trmD"
                     /locus_tag="RHW33_002147"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV1645646.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13893..14441)
                     /gene="rimM"
                     /locus_tag="RHW33_002148"
     CDS             complement(13893..14441)
                     /gene="rimM"
                     /locus_tag="RHW33_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV1645647.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14460..14708)
                     /gene="rpsP"
                     /locus_tag="RHW33_002149"
     CDS             complement(14460..14708)
                     /gene="rpsP"
                     /locus_tag="RHW33_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV1645648.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14845..16206)
                     /gene="ffh"
                     /locus_tag="RHW33_002150"
     CDS             complement(14845..16206)
                     /gene="ffh"
                     /locus_tag="RHW33_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV1645649.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16373..17164
                     /locus_tag="RHW33_002151"
     CDS             16373..17164
                     /locus_tag="RHW33_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV1645650.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17186..18472
                     /locus_tag="RHW33_002152"
     CDS             17186..18472
                     /locus_tag="RHW33_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV1645651.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18527..19120)
                     /gene="grpE"
                     /locus_tag="RHW33_002153"
     CDS             complement(18527..19120)
                     /gene="grpE"
                     /locus_tag="RHW33_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV1645652.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPDASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19243..20121
                     /gene="nadK"
                     /locus_tag="RHW33_002154"
     CDS             19243..20121
                     /gene="nadK"
                     /locus_tag="RHW33_002154"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV1645653.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20207..21868
                     /gene="recN"
                     /locus_tag="RHW33_002155"
     CDS             20207..21868
                     /gene="recN"
                     /locus_tag="RHW33_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV1645654.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGSINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22017..22358
                     /gene="bamE"
                     /locus_tag="RHW33_002156"
     CDS             22017..22358
                     /gene="bamE"
                     /locus_tag="RHW33_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV1645655.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22420..22710)
                     /locus_tag="RHW33_002157"
     CDS             complement(22420..22710)
                     /locus_tag="RHW33_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV1645656.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22700..23137)
                     /gene="ratA"
                     /locus_tag="RHW33_002158"
     CDS             complement(22700..23137)
                     /gene="ratA"
                     /locus_tag="RHW33_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV1645657.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLNGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23308..23790
                     /gene="smpB"
                     /locus_tag="RHW33_002159"
     CDS             23308..23790
                     /gene="smpB"
                     /locus_tag="RHW33_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV1645658.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24005..24367
                     /gene="ssrA"
                     /locus_tag="RHW33_002160"
     tmRNA           24005..24367
                     /gene="ssrA"
                     /locus_tag="RHW33_002160"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24572..25813
                     /locus_tag="RHW33_002161"
     CDS             24572..25813
                     /locus_tag="RHW33_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV1645659.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKKRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEIDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAILEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWADF
                     VMAADHGNMIGDGIRGIRLVG"
     gene            complement(25884..26747)
                     /locus_tag="RHW33_002162"
     CDS             complement(25884..26747)
                     /locus_tag="RHW33_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645660.1"
                     /translation="MFSIEIFSEEISRKRSALSSEIKDCEWRMYAQILDEKHTYMQAE
                     LTMMKERAWNSKKFGPGILLYSSSYLKKHRYTDSHKDFPSNEKSIELATLIERYWSSL
                     EAELLRDNVAVIIPEYISMDELFKFHLRYAQKCYKWGELVISDNEDVAFEAFKKASEL
                     FDKSMGMVWYKTSVDKRNKLLSIRKESGRKGGMKKAEVYGIIQGKLVELINVLAPEQG
                     WGSKAAAVRVLIEPLWEFVEESDFMIDNQSKKYRLATASQDALAETIIKSWSQNNESI
                     KQAFDANVRKK"
     gene            26873..27085
                     /locus_tag="RHW33_002163"
     CDS             26873..27085
                     /locus_tag="RHW33_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV1645661.1"
                     /translation="MNTSSAVRILRLPAVIHKTGISRATIYDWLNPRSPRYDPTFPKK
                     RKLGTQSVGWLESEIDEWLLHRSLIN"
     gene            27112..28626
                     /locus_tag="RHW33_002164"
     CDS             27112..28626
                     /locus_tag="RHW33_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV1645662.1"
                     /translation="MSKKNSSTQNKELGLKFQTLPTFAQRLISYIHYKTGAAAELILI
                     VLLGVMALACQDKFDIQLKNGRTFTSLYLMLLARSGSRKSTVFRLLMGEIYQLEKELR
                     NDFLLKEKIHKLKLVSWETELKELKKQFGKAVRQKVDVTEALEALEECQRRKPSVPER
                     KYLTKNDSTSEGLRKALALGSPSLMLGSDEAGGVIDSNLFRNISVFNSLWGEGRISDS
                     RASRDSYDIDDVRLTVLLLLQPELFNDFISKQGKKLRKSGFLARLLLIDLEQLPELCN
                     IPDACSWSDEPGLDGFFSILVKHLQDGIERREKNEERICITLSEEAKASWDTQSKRIR
                     ELMQKGEELHHYDDFASRIMEQATRIAAVMQMFITPDSPIITKETLMSATKITQWIIN
                     HLITKVDTTRKPTTAEKLVWFMEEHLVSNGSYDFKRNDIIKKGPYSVRRSESLNSALE
                     KLESEGIVQLFERGGVNYVKFIGSKMEPSELAEKTNIPLLDSGTVAMSKLRLPE"
     gene            28888..29586
                     /locus_tag="RHW33_002165"
     CDS             28888..29586
                     /locus_tag="RHW33_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV1645663.1"
                     /translation="MESQYSLTNDEIDDLVEIASRKYSSPIDLNILNPLLNMVYGTLE
                     QNNRILGIRSDLRFAQSHVPGEPDLPLCFQRDDAQAITRSFESLKSQLRADHNRSGRP
                     GNPTLLSYGWCRERDTSEHPHYHLMLLFNADVYGYLGNYQELNAKNMATRIQKAWCSA
                     IGLAHEDYSTLAEFPPNAVYRFSRFDALDRSTVYWDFLIRLAYLAKTRTKDAHSGYRN
                     FGASQCPRLGGLAC"
     gene            complement(29948..31102)
                     /locus_tag="RHW33_002166"
     CDS             complement(29948..31102)
                     /locus_tag="RHW33_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDV1645664.1"
                     /translation="MANEFWIAGAGSGKTYKIIQDAIKVIEDGGRVLVVTYTTNNQAE
                     LRSRFVELYGKSSDDFVVKGLFSFYLEDLVRPYQNVVFPERISTTAFTEHNPHLRPGT
                     NNWHPNRGEKIGGALNPLHYLTPCKTKAYTGLLAKLATRIASSTKNAPAKRLKDIYHR
                     VFFDEVQDLVGWDYDVIKALSKIMPDTICCVGDFRQTIYTTTFGHKAPQTPAQKIHYF
                     QKMNFEQRSLPRNRRCIQKICDLSDTIHAGLYEKTLSEVENVPEEMVHHYGAFVIIPS
                     QVSDYLAMFKPQVLRWSSSMGRAYLPPDLACHTFGSSKGLGFDRVLIISPEKHLKYLG
                     GDLRVFDNDKTEESRNKLYVAITRARYSLAFVVEEEHLADIKLPRWVGLV"
     gene            complement(31095..32753)
                     /locus_tag="RHW33_002167"
     CDS             complement(31095..32753)
                     /locus_tag="RHW33_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1645665.1"
                     /translation="MRVKSIKLTNFKKFKDDHLEFNDDVNIFVGDNNAGKSTILEALE
                     IVLNYNYRGRPFNSEFTPDIFNKDAVQQFLASDKSAKYLPSLTLEAFIDGVPEYRGSN
                     NSLKVDAQGITVQVRFDATLEDVYADYLQTNPNITSIPIEFYKLEWLDFGWNPIKAVA
                     KKFKALYIDPTRIHPTLGKNQYISTILNTALNKEELAKLTLNYRENQQVFNNSGEVKM
                     VNTGLDTDHLITDKKLSIAASTLPAGSIQTSLQLEVDDVPFQFIGKGEQSNVQIKLAI
                     QNKSKDIDLVMMEEPENHLSHTNLNKLVHYIERQRGTKQLFLTTHSSYVLNKLSIDKI
                     CLVHSGYKRLHKLDPAVVKTLKRLPGYDTLRVALARNVILVEGPSDELVLKKIYHRKH
                     DRLPEQDGIDIIVVRGVGFDTFISVGMEIGTSINVLRDNDGAYDENVVKARDAYAAYP
                     NIKLISSAKNEEFSLEPAMIYANASDLKTLNAFAKEVLSKQTYNLYAEVAGLNKRREF
                     LIEWFRSVKGNGKGARKVDSAIKLFDGNLDFKYPAFLDEVFNFG"
     gene            complement(32808..33263)
                     /locus_tag="RHW33_002168"
     CDS             complement(32808..33263)
                     /locus_tag="RHW33_002168"
                     /inference="COORDINATES: protein motif:HMM:NF014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV1645666.1"
                     /translation="MDDVVNGYWILRGIQQQIALAKDNENTLTERLMLIKARTDGGAL
                     DETDFERQRSELAATIANLIELQSQEGVATNQLLLMLNEHPGALKDLLQYKSDSLLPG
                     ALPQLALGIPSDVALNWPDIMACLYRLLQYAYRRKVTSSLQVDTGGSAR"
     gene            33586..34686
                     /locus_tag="RHW33_002169"
     CDS             33586..34686
                     /locus_tag="RHW33_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HDV1645667.1"
                     /translation="MALAHLMRDTLSLVKRDGSRTDGIKGNVQKDKIYIYRSDIAIGE
                     GDLLIRSMPHGGVEEYVVIEPNFRQGLGSIPAGYQAEVRRTTEATTGHVISSTSTAVP
                     ESVISLVQSFIALCITIVNDDASHPEKHVTAYHSLRQEILELNNVMTIPSWIHFSQTP
                     AAVKNSVGLNVQGGSGGWDRRRQYLQSEMMNMLSSYNNSSFAGHSVSINTIKTTEQVS
                     GLTTMASDSVRNVKKKVFIVHGHDEHLKNEVFIFLSKEGFEPVILHHEINEGLTIIEK
                     LEKHIDTVSYAVVLYTACDKGKARNDAELSDRARQNVVFEHGWLISKLSRKNVAAIVE
                     ESVEFPGDLSGLIRIPKSEWKYQLSKELSVIR"
     gene            34723..35853
                     /locus_tag="RHW33_002170"
     CDS             34723..35853
                     /locus_tag="RHW33_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HDV1645668.1"
                     /translation="MAYTRNQHVLSQWVLRNFRSDDTALRPKDKQRVWCHVVVPSAEG
                     ENDIKDIPLPISSVAICKDCFRLKDRDTGEFFDIEHELSEYEQDMSVLVRELVQHHQF
                     ARLANCHIHDFPVEKLSSFAVLQMILNLNNPQSRFPDKDKLKATLITPLLNNIDEHIS
                     AALSLPVTQPALASEPIYQKLMRIAGSSSEVEEKAHAMFVVYSLLALQKKPTPVGTAA
                     YLRDEIFSGIYGIDVFHTGHELNSTEPRPVFTVSPNVFCPLIDEKVIYLPLSHNFALR
                     FNQRPGKGFYSDPIIRIFSPAPQVLCCENHSQVRIYQCSYDYIDQVMSTIDMFNVGFS
                     NIIYSSWQLSDVENYLRLQDEHPDTYYLPERPVRWAAVADKS"
BASE COUNT         9560 a         9067 c         8311 g         9265 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctgaattgc acgtttcaga
      241 ggacgtgcac catagaccgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttat acagacgttt caattgaatc
      361 tgcgcaatcg acgcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcgata
      421 cggttaatga attccggacg gaagttatgg ctaaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcagat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgccca
      601 tcatccagta cctgcaacag aatattgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct ctgttttccc cacaccagtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccagccc cgcacggcta cgacgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaattac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtcac ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccagacg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacc
     1981 cggcggcctt tcaacccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cagctcgttc ggtaagatcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgcccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgttcaac accgacgctc
     2941 gccagacgct gccgggcaag cagataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgtac tcgctttggc gtctgctgcc ataaacgcct cgcgaacctc cgcccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg caactgtcgc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc aggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagtag agctacaggc cgcaacacct tttgtcagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatctttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgata ccggagatcg
     3661 gatgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaattta cgcagcgtgg
     3721 agataaacgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 ttggttcatc tactgtaccg ccagcggtca tatgaccaat tgccaccgct tcatactcac
     4021 gagtaatttc acgcctttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgcct gcacgcggta
     4141 catcagcaat gggcggatag taatgaagta acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc agatcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cgttgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgtccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctat gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactcag tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggca tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa cacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tattgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagatttcca tcacacattt tgacatcagg aacggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgta
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctcgcagagc ggcgcgaact ggccgtcgag
     6361 gtgggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccaacaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggctcc
     6601 tattcacatc ttgccgctcg tcagtacgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaatg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttagtc
     6841 tccggcacga ctgatttatc caccatcaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatccg cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc ggattgaagc gaatcagcga
     7081 caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc tgatcaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccgtaagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcaaccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgcaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 cggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttt
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggcttaa tggaaaagcg gccttcagtt
     9601 gtgccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacatttttc tttacataag
     9781 caatttattc gcgctttcaa cccttgattc tgtatagata aatttacacc acctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgccagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttataccc gaggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatgccag cgcaatacga ttaaattaat taaaacccca caaataaatt
    10261 aaacataaga ttttactcat tatgttttat ttctcacaat gatgacgacc gcatgaaata
    10321 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
    10381 atccctgaat gcgtttttct caccgactgt tcttactgct gattatcctg ctgacgggag
    10441 caccaatact cgctcaggaa ccttctgacg ttgcgaaaaa cgtgcgtatg atggtgtccg
    10501 gtattatcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
    10561 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
    10621 ctgtcatcat tcacactcaa caagaggcga tgatttcaga ctgcaatggt ttttattttg
    10681 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
    10741 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctaatcatcc
    10801 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
    10861 tcaacccgga tgtcttgatg ctcgcacgga agaaaaatga tggataacga taattctctt
    10921 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
    10981 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
    11041 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
    11101 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
    11161 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcattctg gcatcctggc
    11221 attacacccg taaggatcca ggcgatactt tcagcaattt cataagccac tggctcttcc
    11281 ctgcccccat cattcagccg attcgtcaca atggcgaaac cattggcgaa gtacgcttaa
    11341 ccgctcgcga cagttcaatc agcaatttta tctggttttc gctcgccgta ctgaccggtt
    11401 gtattctgct ggcatcagga atcgcaatta ccctcacccg ccatttgcac aatggcctgg
    11461 tggaagcact gaaaaatatc accgatgtcg tacatgatgt gcgttccaac cgcaattttt
    11521 cccgacgagt ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca
    11581 gtctgctgga tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc
    11641 tgcgtaccgc gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg
    11701 gcatcaatac gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg
    11761 atggcgataa tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct
    11821 tgattgaaat cgcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc
    11881 tgggcggcga tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc
    11941 agatatgctc agcactgaca caaatcttta atcgcccgtt tgatcttcat aatggccatc
    12001 tgaccactat gacattaagc attggttacg cgatgaccat tgcgcacgcc tctgcggaaa
    12061 aattacaaga gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccaaaaagc
    12121 tggtgagata acaaggatat atcgatatga taaagcacct ggtagcaccc ctgattttca
    12181 cctcactaat actgactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg
    12241 ccgctatgca atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag
    12301 atgccattct gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacagatcc
    12361 aaaccatggc agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca
    12421 ccgataacta tggtgaagat agttacaacg aaggcttatc attgaaacgg gcgaatgtcg
    12481 tggccgatgc atgggctaag ggtggacaaa ttccgcgcag caatctcacc acacagggtt
    12541 taggaaaaaa atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc
    12601 gcgtcgcagt ggtgattact accccttaaa acacgagcaa acagataaaa aaagggccag
    12661 ccaattggcc agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc
    12721 tttgatacga gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac
    12781 agcaccacga cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg
    12841 ctcaacgcct tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt
    12901 acgaatagcg ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac
    12961 ccatactttc acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg
    13021 ctcttgttca agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact
    13081 gatatctcgg gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt
    13141 ttgaactccg ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca
    13201 ggtcttctaa gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca
    13261 tggtttcccg acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta
    13321 tagtgcgggc aatccagcaa tccttcagca aaggaatctt ccgttgccga ggcttcatgt
    13381 cccagtactc ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca
    13441 ccaccactga gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg
    13501 cgctcatcta taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagt
    13561 tcgctgacgc ccgcttgatc aagcttgcgt ccctgtggtg aaagataaat cacctttgcg
    13621 ccttcacccg ccgcggcttt tgctgcatga atggcgtccc gcaagggttg caccatcatt
    13681 aacatccccg gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgcgtg
    13741 aaatcgcgag gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt
    13801 accccgtaat cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac
    13861 atagcgccgt cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg
    13921 attgaacgag tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga
    13981 cgttccttga taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat
    14041 ccggtttcca tcatatcgac gactttaccg agatcgtagc cttcagtggt tactacctgg
    14101 caacccatca ggtctttcca gtagtagtca ccctcttcaa gctgaggcag ttgcgatgaa
    14161 tccacgacaa tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc
    14221 agcttgatga tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac
    14281 tgacccgcct tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg
    14341 gaagaaaaca ctctgagcca cccacgaata ccgtaagacg aacccatttt tcccaaaacg
    14401 atgggatcaa caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat
    14461 taagctgctt tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc
    14521 tggccaaccc agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta
    14581 gcgattgggt tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg
    14641 tcagcgacaa caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata
    14701 gttaccataa catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc
    14761 ggtgtcatat taaaagcccg aaaattttac tgatttctgg agaaaatgca atcagcagtt
    14821 aataactctg tcgcataagg tcatttagcg accaggaaat cctggtggca tcatcccttt
    14881 catgcttctc atcatcttcg ccattccgcc cttcttcatt ttcttcatca tgcgctgcat
    14941 gtcgtcgaac tgtttcagaa gacggttaac gtcctgcacc tgcataccgc aaccggcagc
    15001 aatacggcgt ttacgcgaac ctttgatgat ttctggctta gcgcgctctt tcatcgtcat
    15061 cgagttgatg atggcttcca tacgcaccag cactttatcg tccatctgtg acttgacgtt
    15121 atccgggatc tgccccatgc ccggcagctt gcccatcaga ctagccatgc cgcccatatt
    15181 tttcatctgg cgcagctgct caagaaagtc gttgagatcg aagccgtcgc ctttttttag
    15241 cttgctggct aatttctctg cctgcgcgcg gtcaactttg ctttcgatat cttcgatcag
    15301 cgacagtacg tcgcccatgc cgagaatacg cgacgcgatg cggtccggat ggaacggctc
    15361 cagcgcctca gttttctcgc caacaccgag gaacttgatc ggtttgccag tgatgtgacg
    15421 aatagagagc gccgcaccgc cgcgggcatc gccgtccact ttggtcaaca ctacgccggt
    15481 cagcggtaac gcttcattga atgcttttgc cgtattggcc gcatcctgac cggtcatggc
    15541 gtcaaccaca aacagggttt caaccggatt tatggacgca tggacttgtt tgatctcgtc
    15601 catcatcgct tcgtcaacgt gcagacgacc agcggtatcc accagcagca cgtcgtagaa
    15661 tttcagtttg gcttctttca gcgccgcgtt aacgatatct accggcttct gaccaacatc
    15721 agaagggaag aaatcaacgc ccacctgctc tgccagcgtc tcaagctgtt tgattgccgc
    15781 cgggcgataa acgtcggcag aaaccaccag cactttcttc ttgtgcttct cgcgcaggaa
    15841 cttaccgagt ttaccaacgc tggttgtttt accggcacct tgcaggcccg ccatcagtac
    15901 gaccgcaggc ggttgcgcag ccaggttcag ggtctggttc tcttcgccca tcgccgcaac
    15961 cagttcgtta cggacgattt tgacgaactc ctgccccggc gtcaggctct tattaacttc
    16021 atgaccaacc gctttctctt ttacgcgatt gataaactca cgcactaccg gcagagcgac
    16081 gtccgcctcc agcagcgcca tgcgcacttc gcgcagcgta tcttttacgt tgtcttcagt
    16141 gaggcgtcca cggccactga tattgcgcag cgtgcgcgac aaacgatcgg ttaaattatc
    16201 aaacattgtc tctcgcctgg ggtggaaacg gttggccgca atcgcgacac atcatcagta
    16261 ttttgccgca gtataacatg aaggcgtctt tgttgttatg caacggttgg agcagcgttc
    16321 acctgacgct atactgcttc tctttcttat tgctcaaact gtcgacatca ctatgcccgt
    16381 ttttgctctg ctcgcgcttg tcgcctactc cgtcagtctt gcgctgattg ttcccggtct
    16441 gctgcaaaaa aacggcggct ggcggcgcat ggctattatt tctgcggtca ttgcgctggt
    16501 ctgccacgca atcgctctgg aagcccgcat cctgcccgac ggtgatagcg gacaaaacct
    16561 cagcctgctg aacgttggtt cattggtcag tttgatgatc tgtacggtaa tgaccattgt
    16621 ggcctctcgc aatcgtggct ggttgctgct acccattgtc tatgcctttg cgcttatcaa
    16681 cctggcgctg gcgaccttca tgcccaatga atacatcacc catctggaag ctacgcctgg
    16741 gatgctggtg cacattggct tatcgctctt ttcctatgcc acgctaatta tcgccgccct
    16801 gtacgcgctg caactggcgt ggattgatta ccaactgaag aacaagaagc tggcgtttaa
    16861 ccaggaaatg ccgccattga tgagtatcga gcgtaaaatg ttccacatca cgcagattgg
    16921 cgtggtgctg ctaacgctca cgctttgcac tggcctgttc tacatgcaca acttatttag
    16981 catggaaaat atcgacaagg ctgtgctctc tatcgtggcg tggtttgtct atattgtgct
    17041 gctgtgggga cattatcatg aaggatggcg tggacgtcgc gtcgtctggt ttaacgttgc
    17101 gggtgcagtc attctgacac tggcctactt cggcagccga attgtccagc agttaatcag
    17161 ctaaacccag aaaaggagtt tccccctgga acacatttct actactacgt tgatcattat
    17221 tctgatcatc atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac
    17281 cctcaaccgc tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt
    17341 cgaaaaattg ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct
    17401 ggtcaatatt cttgcctccg cgctcggcac tattgttggg atgcgtttgt acggcgatgc
    17461 gggcgtggca attgcgactg gtgtgctgac ttttgtcgta ctggtttttg ctgaggtatt
    17521 gccgaaaacc attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct
    17581 ggctccgctg caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat
    17641 gctgatgcgc atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga
    17701 agagttgcgc actatcgtgc acgaatcgcg ctcacaaatt tcccgtcgca atcaggatat
    17761 gctgctgtcg gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag
    17821 tgaaattatc ggtattgata tcaacgatga ctggaaatct attctgcgcc aactctccca
    17881 ctcacctcac gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct
    17941 gcgagtacgt gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat
    18001 gctgcgcgcc gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt
    18061 ggtgaagttt cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat
    18121 tcaggggctg gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc
    18181 gatgtcgcca acacttgccg aagaagtcac gccgcaaaac gacggttcgg tgattattga
    18241 tggcactgcc aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga
    18301 tgcccgcacg gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac
    18361 ccgcgtgcgt attggcgagt acgatataga tattctcgac gtacaggaca atatgattaa
    18421 gcaggtaaaa gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg
    18481 gccctgcatt cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt
    18541 tcgctacagt taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca
    18601 taatgcccag tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat
    18661 gcacattcgg gtccagcggc acgttagttt cggcgatcac ttcaacgcca aacttacgca
    18721 caacatccag catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg
    18781 ggttagcttt atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt
    18841 cgttgatgaa tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgac
    18901 gacgcaggtt ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg
    18961 cttcagccag ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag
    19021 cagaagcgtc tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg
    19081 gggcttgccc ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt
    19141 cgcattcatc tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc
    19201 actcacattg tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag
    19261 tgtattggca ttgtgggaca cccacggcac cctactgcac tgacaacaca tgaaatgctc
    19321 taccgctggc tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa
    19381 ttgcaactga agaatgtgaa aactggcacg ctcgcggaga ttgggcaact ggctgatctc
    19441 gcggtagtcg ttggtggcga cggtaatatg ctgggcgcgg cgcgcacgct cgcccgttac
    19501 gatattaaag ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc
    19561 gataacgccc agcaacaatt agccgatgtg ctggaaggtc actacatcag cgagaaacgt
    19621 tttttgctgg aagcgcaagt ctgtcagcaa gattgccaaa agcgcatcag caccgcgata
    19681 aatgaagtgg tacttcatcc aggcaaagtg gcgcatatga tagagttcga agtgtatatc
    19741 gacgagatct ttgcgttttc tcagcgttct gatgggctaa ttatttcgac gccaacaggc
    19801 tccaccgcct attccctctc tgcgggcggt cctattctga ccccctctct ggatgcgatt
    19861 accctggtgc ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc
    19921 agcacgatcc gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc
    19981 cagatagcac taccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat
    20041 ctgaatctga ttcatccgaa agattacagt tatttcaata cattaagcac caagctcggc
    20101 tggtcaaaaa aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta
    20161 tactgtacac aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact
    20221 gaccatcagc aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac
    20281 cgtaataact ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggtctttg
    20341 tctcggtggt cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg
    20401 cgcccgtttt tctctgaaag atacgccagc ggccctgcgc tggctggaag aaaaccagct
    20461 tgaagacggg catgaatgtt tgcttcgtcg cgtaatcagc agcgatggtc gctctcgtgg
    20521 ttccatcaac ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat
    20581 tcagatccat ggtcagcacg ctcatcagtt actcaccaaa cccgagcacc aaaagttcct
    20641 gcttgatggc tatgccaatg aaacctctct actccaggaa atgaccgcac gttatcagtt
    20701 gtggcatcaa agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgctgc
    20761 ccgtgcggaa ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg
    20821 agagtttgag caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac
    20881 caccagccag aatgcattgg cattaatggc cgacggtgaa gacgcaaacc tgcaaagtca
    20941 gctttacacg gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg
    21001 cgtacttgat atgctggaag aagctaccat ccagattgct gaagccagcg acgaactgcg
    21061 ccactactgc gatcgtttgg atctcgatcc caaccggctg tttgaacttg aacaacgcat
    21121 ctcaaaacag atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat taccacagta
    21181 ttaccagtcg ctactggaag aacagcagca actggacgat caggccgact cacaagaaac
    21241 gctcgcgctg gcggtaacga aacatcatca gcaggcactg gaaaccgcgc gcgcattaca
    21301 ccaacaacgc cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc
    21361 gctctcaatg ccgcatgggc agtttacgat cgatgttaaa tttgacgagc atcacctggg
    21421 cgctgacggt gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca
    21481 gcctattgcc aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caatccaggt
    21541 catcacggcg cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat
    21601 tagcggtcca acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca
    21661 ggtgatgtgt gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt
    21721 cagcaaagaa accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaagc
    21781 gcggttacaa gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta acacactggc
    21841 gaatgcgaaa gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca
    21901 gcaaaacgcc gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat
    21961 cggcatatta cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc
    22021 gctgtaaaac gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca
    22081 ctctggagcg agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg
    22141 acgtatccaa aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac
    22201 cgctgatgtc cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag
    22261 gtcatgaagg tgtaacgcag caaacgctga cgctaacctt taacagtagc ggtgtgttga
    22321 ccaatattga taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac
    22381 gttaaaaaag gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc
    22441 tgctcgttgc ctgcgcagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg
    22501 atcgccatca tgcacactat cgctcagttt tgccggacgg ctataaatgc cgactttgtt
    22561 tttagttaaa tcgatatcgg tccgcaattc cagcaagcca ctggcgcgaa tagcttcttc
    22621 aaccgtcgcg ccctcctgca gcgtcacgcg ctgcaggtac tgcttctcag gtagcgcata
    22681 agccacctca acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag
    22741 cctggaccat attagacgcc agctctttaa acacgcgacc aaaggcgagc tcaatcaact
    22801 tattggtaaa ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg
    22861 taaacttcca tccaccgttc aatttcttga acggcccatc caccagattc ataagaatac
    22921 tttggttact ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga
    22981 catctaccgc cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac
    23041 ccggcaaaaa ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc
    23101 tgtagggtac cagtgcggtc cggctaatct gaggcataac aatttccatc aacaaaaatc
    23161 caacaaataa tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat
    23221 cgggttcatg ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt
    23281 tcagattacc gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca
    23341 gcgaccatcg cgcttaataa gcgcgcccgt cacgaatact ttatcgaaga agagttcgaa
    23401 gcgggacttg ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc
    23461 agcgacagct acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg
    23521 ccaatggccg tggcctccac gcatgtggtg tgcgacccta cccgtacccg caagttactt
    23581 ctcaaccagc gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta
    23641 gtggcgctct ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa
    23701 ggtaagaaac agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa
    23761 gcacgtatca tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc
    23821 accgcgcctc cctctccggc ggcgcgaatg aacatcttat tggctattac atccgacaca
    23881 aatgttgcca tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt
    23941 aacgataacg ccattgaggc tggtcatggc gctcataaat ctggtatact tacctttaca
    24001 cattggggct gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc
    24061 ggttggcctc gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca
    24121 gcttaataac ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag
    24181 agaggtcaac cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact
    24241 taatcaggct agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact
    24301 gactaagcat gtagtaccga ggatgtagga atttcggacg cgggttcaac tcccgccagc
    24361 tccaccaaaa ttctccatcg gtgattacca gagtcatccg atgaagtcct aagagcccgc
    24421 acggcgcaag ccctgcgggc ttttttgtac ctgcaatttg tcccgcgaag tctgatgcca
    24481 actaattaaa tccgaacctt ttaggcacct tgttaggcac ctcgtaaagc tttattgttt
    24541 ttgaggtgcc taaaactatg gaaacccggc aatggcaaga caaaccaaac ctctatccgt
    24601 taaagaaatc gaatctgcta aacccaagga agcggactac gttctctatg atggcgatgg
    24661 ccttgagcta ctgattaaat ccagcgggag taaaatctgg cagtttcgct acattcgccc
    24721 tgtcaccaag aaacgtgcga agaagagcat aggcccctac ccgtcagtta cgcttgccga
    24781 tgccagaaac tatcgagcag aatctcgctc tctcctggcg aaacaaatcg atcctcagga
    24841 gcatcagcaa gaacaactgc gcagttcgct tgaagccaaa acaaatactt tccagctcgt
    24901 agctgaacga tggtggaatg tgaagaaagc cagcgtgaca gaggactatg cggaagatat
    24961 ctggcgctct cttgaaagag atgtctttcc tgcgattggc gacgttagcg ttacagatat
    25021 taaagctcac acactggttc aggccgtcca accggttcag gccagaggag cactggaaac
    25081 cgttcgtcgc ctgtgccagc gcattaatga ggtcatgatc tatgcccaaa acacagggct
    25141 gattgatgct gttcccagcg ttaatattgg taaagccttc gagaagcctc agaaaaagaa
    25201 catgcccagc attcgaccgg atcagctacc tcaattgatg cagacaatgc gaacagccag
    25261 cattagcctt tccacacgct gcctgttcat gtggcaactt cttactatta cccgccctgc
    25321 cgaagcggct gaagctcgct gggaagagat agacatagaa gcacaagagt ggaagattcc
    25381 tgcagcacgc atgaaaatga accgcgacca tactgttcca ttgtcagatg aagcaattgc
    25441 gatactagag atgatgaagc cgttaagtgg aaatcgagaa tttatctttc ccagccgcat
    25501 caagccaaac caacctatga acagtcagac cgtaaacgca tcgctaaaac gcgcaggttt
    25561 tggtggagtg ctcgtttcac acggtctgcg atctatcgcc agtacagccc ttaatgaaca
    25621 aggctttccg cctgatgtta ttgaggcagc actggcccac gtggataaga atgaggtacg
    25681 tcgagcatat aaccgcagcg attatcttga gcagagacgc ccgatgatgc agtggtgggc
    25741 tgacttcgtt atggctgctg atcacggaaa tatgattgga gatggcattc gaggtataag
    25801 gctcgtcgga tagtatttat ataagcgcct caaactggcg cttatatctt tcactcaaat
    25861 ccgaagctcc gattaataac tgattatttt tttcttacat tcgcatcaaa agcttgttta
    25921 atactttcat tattttgcga ccaagatttt atgatagttt cagctaaagc atcttgacta
    25981 gcagttgcta atctatattt tttactttgg ttatcaatca taaaatctga ttcctcaaca
    26041 aattcccaca aaggttcaat gagaactctc acggcagcag ctttacttcc ccatccctgc
    26101 tctggggcta aaacattaat aagctccaca agctttcctt ggataattcc ataaacttca
    26161 gcttttttca tccctccttt tcgcccagac tcctttctga tacttaaaag tttatttcgt
    26221 ttatctacag aagttttata ccaaaccatc cccatgcttt tatcaaataa ctcagatgct
    26281 tttttaaatg cttcaaatgc aacatcctca ttgtctgata taactaattc accccattta
    26341 tagcacttct gtgcatacct aagatgaaat ttaaagagtt catccataga gatatattca
    26401 gggataatga cagcaacatt atccctcaga agttcagcct ctaatgaact ccaataacgt
    26461 tcaataagcg tagctaactc aatgcttttc tcatttgatg gaaagtcctt atgagagtca
    26521 gtgtacctat gctttttcag ataagaggat gaatacaata atattcccgg accaaatttt
    26581 ttggagttcc aggcccgctc tttcatcata gtcagttcag cctgcatgta tgtatgcttt
    26641 tcatccagaa tttgggcata catgcgccat tcacagtctt taatctcact ggagagagca
    26701 ctgcgttttc gactaatttc ttcactaaaa atttcaatag aaaacattta gccccacaat
    26761 atcaacaagt taaacctgtt aaaaataaac ctttgctagc cgtatttttt cgaagaatcc
    26821 ttgcagctat tctttgctgg aaccaaactg agctaacaag aggcaaacta gtatgaacac
    26881 ttcatcagca gtcaggatac tccgcttacc tgctgttatc cataaaacag gtatctcgcg
    26941 agccacaatt tatgactggt tgaacccgag gtcgccacga tatgatccaa cttttccgaa
    27001 gaaaagaaag cttggaacac aatcagttgg atggcttgaa tcagaaattg atgaatggtt
    27061 gttacatcgt agtcttatta attaattata aagaggagtt agataggatg tatgtctaaa
    27121 aaaaacagct caacgcaaaa taaagaactt ggactaaagt tccagacgct tcccactttt
    27181 gcacaacggt taataagcta catacattat aaaacaggag ccgccgctga acttatttta
    27241 atagtgttac ttggggtaat ggcacttgcc tgccaggaca aatttgatat tcagcttaaa
    27301 aatgggagaa cctttacatc actttatctg atgctattgg cacgctcagg tagcaggaaa
    27361 tcaacggtat tcaggctgtt gatgggggag atttatcaac tggaaaaaga gctgcgaaat
    27421 gattttctgt taaaagagaa aattcataag cttaaactag tatcctggga aaccgagcta
    27481 aaagagctta aaaaacaatt tggcaaagct gttcgtcaga aggttgatgt aactgaggca
    27541 cttgaggcgt tggaggagtg ccaaagaaga aaaccatctg tcccagaaag aaagtatctt
    27601 actaaaaatg attcgacaag tgaggggctt agaaaggcat tagcgctggg ttctccatca
    27661 ttgatgctcg gctctgatga agccggtgga gtgattgaca gcaatctttt tcgcaatata
    27721 tcagttttta actctctttg gggagaaggg agaatctctg acagcagagc atctcgtgac
    27781 agctacgata ttgatgatgt ccggctgacc gtattgctgt tgttacagcc tgaattgttt
    27841 aacgacttta taagtaagca ggggaaaaaa ttaagaaaat caggattctt agcgcgttta
    27901 ttattgattg atctggagca acttcctgaa ctatgcaaca tccctgatgc gtgttcatgg
    27961 tctgatgaac caggacttga tggttttttc tctattctcg ttaagcattt gcaagacggg
    28021 atagagcgta gagaaaaaaa tgaagaacgt atctgtatta ctttatctga agaggcgaaa
    28081 gcgtcatggg atacacaaag taaacgaatc agggaactaa tgcaaaaagg agaggagttg
    28141 catcactatg atgatttcgc ctcaagaatt atggaacaag ctacgagaat cgcagcagta
    28201 atgcaaatgt ttattacacc cgactcacca ataattacca aggaaacgtt aatgtcagct
    28261 actaaaataa cacaatggat tattaatcac cttatcacta aggtcgatac tacccgaaaa
    28321 cctacaactg cagaaaaatt ggtatggttc atggaggagc atcttgttag taatggttct
    28381 tacgatttca agaggaatga tatcattaaa aaaggtccat attcagtcag gcgttcagaa
    28441 agtctgaatt ctgctttaga aaaacttgaa tctgaaggaa tagtacaact atttgaacga
    28501 ggtggggtta attatgtcaa atttattggt tccaaaatgg aacctagtga actagctgaa
    28561 aagactaata tcccgttgtt agattcagga acagttgcga tgagtaaact acgtttacct
    28621 gaataacttc agagtaagca ggctcttcct gattaatatt tattttgttc ttaagccgag
    28681 taacactaag gattcagctt aaattattga ggaggacagc tcatgagttt taatcggtat
    28741 atgtaatata cctttatcaa aggtctactc ctgcacaaac aatcatcaag agtgacaacc
    28801 cctacgggga ttatatccaa catcacatgg gtagtgtgag agctacccat tatctaatga
    28861 ttaatattta cagaatgaag gattattatg gaatcacaat attcgcttac caacgatgaa
    28921 attgatgact tggtagaaat agcctccaga aagtatagtt cacccattga cctcaatatt
    28981 ctcaacccac tacttaacat ggtctacggc actttggagc agaataaccg aatactaggt
    29041 attcgttctg atctgagatt tgcccagtct catgtaccag gagaacctga tctacccctg
    29101 tgcttccagc gcgatgatgc acaggcaata actcgctcct tcgagtcact gaaaagccag
    29161 ctacgcgcag atcataaccg gtccgggaga ccgggtaacc caacgttgct atcttatgga
    29221 tggtgcagag aacgtgacac aagcgagcat ccccattacc acctgatgtt gttgtttaat
    29281 gcagatgtgt atggatactt gggcaattac caagaactca atgctaaaaa tatggcaacc
    29341 cgaatacaga aagcctggtg cagtgctata gggcttgctc acgaagatta ctcaacatta
    29401 gctgaattcc cacctaatgc tgtttacagg tttagcagat tcgatgcttt ggatcgtagc
    29461 acagtctact gggatttcct gattcgactg gcgtacttag ccaaaaccag aactaaggat
    29521 gcccacagcg gttaccgcaa ctttggcgca agccagtgtc cacgcttagg cggattagcc
    29581 tgttaatagc actctgggtg aggtatttat cctcaccctg tccattaaca taattgatac
    29641 atttctcggt aaaaaattcg atacagtgtc tcggtaccct gctacaacct gctacatgta
    29701 gcagctgtag cagctttcag agatcaggac atacattttt gggctaataa ttacaggtat
    29761 gacgctgtct aagaactagg ttccttattg atgattgcat caagttggca atgtccgctt
    29821 tggcactgac caacctccca tctggagcaa cgccctcctt ctttaaggat agtgttgcat
    29881 cgccccgtta aactcacagc gaaaaatgga agttaaggaa gttagatggt tagacgttag
    29941 catcaactta cacgagccca acccaacgtg gcagtttgat atctgctaga tgctcttcct
    30001 caacgacaaa cgccaagctg taacgagctc gggtaatagc cacgtacagc ttgtttctag
    30061 actcctcagt tttatcgttg tcaaataccc tgaggtcgcc gcctagatac ttgagatgtt
    30121 tctccggtga aataatcagg acacgatcga aaccgagacc tttgctggag ccaaaggtgt
    30181 gacaggccaa gtcaggcggc aaataggctc tccccatgga tgatgaccat cgcagaacct
    30241 gaggtttgaa catggcgaga taatcgctca cctgtgaagg tatgatgacg aaggcaccgt
    30301 aatgatggac catctcttca ggaacatttt ccacctctga cagagtcttt tcatagagac
    30361 ctgcatgaat cgtatcggac agatcgcaga ttttttgaat acatctgcgg tttctcggaa
    30421 gcgagcgctg ctcaaaattc atcttctgaa aatagtggat cttttgcgca ggagtttgcg
    30481 gtgccttatg gccgaaggta gtagtgtaaa tagtttgacg gaaatcgccg acacagcaaa
    30541 tcgtgtcagg cattatttta cttagcgctt tgataacatc gtaatcccaa ccaactaaat
    30601 cctgaacctc atcgaaaaag acccgatgat agatatcttt caacctcttg gcaggagcat
    30661 tctttgtcga gctggcgatc ctggtagcca gcttagctag taggccggta tatgcttttg
    30721 tcttgcaggg ggtcagatag tggagtgggt ttagagcccc accaattttt tctcccctat
    30781 tgggatgcca attattcgta ccaggcctca agtgagggtt atgctcggtg aacgctgtgg
    30841 tggaaatgcg ctcagggaac acgacatttt ggtagggtct cactaagtct tccagataaa
    30901 aggaaaatag gccttttacg acaaaatcat cactgctttt gccatatagt tccacgaaac
    30961 gagaacggag ctcggcttgg ttgttcgtag tataggtaac caccaataca cgccctccat
    31021 cctctatcac cttaatagcg tcctggatga ttttataggt tttacctgat cctgctccgg
    31081 cgatccaaaa ttcgttagcc aaagttgaat acctcatcga ggaaggccgg atacttgaaa
    31141 tctaggttcc catcaaacag cttgatagcc gaatcgactt tacgagcgcc cttaccgttc
    31201 cccttaacac ttcgaaacca ttcaatcaga aattcgcgcc gtttgttcaa gccagcaacc
    31261 tctgcgtaga ggttgtaggt ctgtttggat aacacttcct tagcgaaagc attcagcgtt
    31321 tttaagtctg atgcattggc ataaatcatc gctggctcaa gcgaaaactc ctcatttttc
    31381 gcagaagaga tgagcttgat attgggatag gcagcatagg cgtcacgggc cttcacgacg
    31441 ttctcatcat aggctccatc gttatcccgg agcacgttga tactggtacc aatctccatg
    31501 cccacggaaa tgaaagtatc gaacccgact ccgcgcacca caatgatatc tattccgtcc
    31561 tgctccggca ggcggtcgtg tttgcggtgg taaatctttt taagcaccag ctcgtccgac
    31621 ggtccctcca ctagaatcac attgcgcgcc aatgccacac gcaatgtatc atagccaggc
    31681 aatcgcttaa gggttttgac gacggcagga tcaagcttat gaaggcgctt atatcccgag
    31741 tggacaaggc aaattttatc aatgctcagc ttgttgagca catacgagct gtgagttgta
    31801 aggaatagct gtttggtgcc ccgctgcctc tcgatgtagt gcaccagctt attaagattg
    31861 gtgtgcgaaa ggtggttttc gggctcctcc atcatcacca aatcgatatc cttcgatttg
    31921 ttctgaatcg cgagtttgat ctgcacgtta ctctgctcac ccttgccgat gaactggaaa
    31981 ggtacgtcat ctacctcaag ttgaagactg gtctgaatag atccggctgg taaggtactc
    32041 gcggcaatgg agagtttttt gtccgtgatc aagtggtccg tatctaaacc agtattgacc
    32101 atcttgacct cacccgaatt gttgaatacc tgctgattct cgcgataatt gagagtcagc
    32161 ttagccagct cctctttgtt caaagcagtg ttcaaaatgg tcgagatgta ctggttctta
    32221 cccaaagttg gatggatgcg tgtcggatcg atgtacaatg ccttgaattt cttggctaca
    32281 gccttaatgg gattccatcc gaaatccaac cactccagct tgtagaactc gatcggaatg
    32341 cttgtaatat tagggtttgt ctgcaagtag tcagcataca cgtcctcaag cgtagcatcg
    32401 aagcgcactt gcaccgtgat cccttgagca tcgaccttca aggaattgtt cgaccctcga
    32461 tattcaggca ctccgtcgat gaatgcctcg agcgtcaagc taggcagata cttggctgat
    32521 ttgtctgaag ccaagaactg ctgaacagca tccttgttga aaatatcagg tgtgaactcg
    32581 ctgttaaagg gacggccacg atagttgtaa ttaagaacta tttccaaggc ttccagaatc
    32641 gtacttttac cggcgttgtt atcgcctaca aagatgttta cgtcatcgtt gaactcaaga
    32701 tgatcatctt tgaatttctt gaaattggtc aacttaatag atttaacgcg cactctaaat
    32761 ccctcaagat actgtcaaat aatgactaaa tgatcccctt aaagacatta cctcgccgat
    32821 ccaccagtgt ccacttgcag gctcgaagtc acctttctcc tgtaggcata ctgaagcagt
    32881 cgatataaac aggccatgat atcaggccag tttaaggcga catccgaagg aatccccaat
    32941 gctaattgag gtaaagcgcc tggcaacagg ctgtctgatt tgtactggag caaatcttta
    33001 agtgcaccgg ggtgttcatt cagcataaga agcaactggt ttgtcgccac accttcctgt
    33061 gactgcagct ctattagatt tgctattgtc gcggccagtt cgctgcgctg gcgttcgaaa
    33121 tcagtctcat caagagctcc gccatcggtg cgggctttga tcagcatcaa acgttcagtg
    33181 agggtattct cattatcctt agccagtgca atttgctgct gaataccccg cagaatccag
    33241 tagccgttta ccacatcatc cacaatgctt aaccggggca agctcaagta gcgccttctg
    33301 aggatcgctg tcagcttcag aggctttcac agcacggctg atgtgtcccc acaagtcaat
    33361 ctcccagata aaatccagtt ccccttggta ccagtcataa ggttcagcca taagttgtat
    33421 cagttcattc ggattcagat gtgcttcttt cagtgctctg gttgaaggac tgttctcact
    33481 ttgttgtgcg cgggtagcgt tcgggaaaga attgagagcg agaagttatt cattccatct
    33541 gctatgctaa cgcccaaatc agtttgacta tttccggggt aatacatggc cttagcacat
    33601 ttaatgcgtg acactttatc ccttgttaag cgggatggaa gcagaaccga tgggataaaa
    33661 gggaacgttc agaaggataa gatatacatt tatcgaagcg atattgctat tggagaaggt
    33721 gatcttctta ttcgcagtat gccgcatggt ggtgttgaag agtatgtcgt tatcgagcca
    33781 aatttcagac agggattagg gagtattccc gcaggttatc aggcagaggt tagacgaacg
    33841 acagaagcaa ctacgggaca cgttatatcg tccacctcaa cggcggttcc ggagtcagtt
    33901 atttccctag tgcagtcgtt cattgcatta tgtattacca tcgtaaatga tgatgcatct
    33961 caccccgaga agcatgttac tgcctatcat tcattgcgcc aggaaatcct tgaactgaac
    34021 aatgtcatga ctatcccttc ttggatacac ttctcccaaa ctcctgcagc agtgaagaat
    34081 agcgtgggtt taaacgtcca gggaggcagt ggtggatggg atagaagacg tcaatattta
    34141 cagagtgaaa tgatgaacat gctttcgagc tacaataatt catcgttcgc cggacattct
    34201 gttagtataa ataccattaa aaccactgaa caggttagtg ggttaactac aatggcaagc
    34261 gattcagtac gtaatgtaaa aaagaaagtc ttcattgttc atgggcatga tgaacactta
    34321 aaaaatgaag tttttatctt tctcagcaaa gaagggttcg agcctgtcat tcttcatcat
    34381 gagataaatg aaggactgac tattatagaa aaactggaaa agcatatcga tacggtttca
    34441 tatgccgttg tgctttatac tgcctgtgat aagggcaagg ccagaaatga tgcggaattg
    34501 agtgaccggg cacgacaaaa tgttgtattc gaacacgggt ggcttatttc caaactctca
    34561 cgtaaaaatg tcgcggcgat cgtagaggaa agtgttgaat ttcccggtga tctttcaggc
    34621 cttatcagaa taccgaagtc tgaatggaag tatcagctct caaaagagtt aagcgttatc
    34681 agataatctc cgggttctga aacaatagtc agaagggaaa acatggcata caccagaaac
    34741 cagcatgttc tttcgcagtg ggttctgcga aactttcgta gtgatgacac ggcactgagg
    34801 ccgaaggata aacagcgtgt ctggtgtcat gtcgtggtgc ccagtgcgga aggcgaaaat
    34861 gacatcaagg atatcccgct acccatatcc agcgtagcca tctgtaagga ctgttttcgt
    34921 ctgaaggatc gtgatacagg tgagtttttc gatattgagc atgagctcag cgagtatgaa
    34981 caggatatgt ccgttcttgt ccgggagctt gtgcagcatc atcaatttgc ccgtctggca
    35041 aattgccata tacatgactt tccagtggaa aaactgtcaa gctttgctgt tttacagatg
    35101 atacttaacc ttaataaccc gcaaagtcga tttcctgaca aggataaatt aaaagcaacg
    35161 ttgataaccc ctttgcttaa taacattgac gaacatatat cagcagcgct ttcacttcct
    35221 gtaactcaac ctgctcttgc ctctgagcct atttaccaaa aactgatgcg catcgctggt
    35281 tcttcatccg aagtggagga aaaagcacat gccatgtttg ttgtttattc cttgctggct
    35341 ctgcaaaaaa aaccgacgcc cgtgggaaca gctgcctatc tgagggacga gatattctcc
    35401 ggtatttacg gcattgatgt ttttcacact ggccatgaat taaacagtac cgaaccaaga
    35461 ccagttttta ctgtttcccc taatgtattt tgtcccttaa ttgatgagaa agtcatttat
    35521 ttaccacttt ctcataactt cgctctgcga tttaatcagc gaccggggaa aggtttctat
    35581 tcggatccga taatcaggat tttcagccca gcccctcagg tcctttgctg tgaaaatcat
    35641 tctcaagtga gaatatacca gtgctcctat gattatattg atcaggttat gtcaactata
    35701 gatatgttta acgtaggttt ttcaaacatt atatattcga gctggcagct cagcgatgtt
    35761 gaaaactatc tgaggctgca ggacgaacat cctgatacct attatcttcc cgaacggccg
    35821 gttcgctggg cagcggttgc ggataaaagc tgatgggttt cactccggaa caagttgccc
    35881 gctgcaggca acgtcacgct atggaaagct ttaatttgaa tagccagctt gccggtgaaa
    35941 gctgagagca tctggatgtg gcacggagcg gactgaagcc aacatcataa ggtctgcaat
    36001 gagcgagaag cggacattga aatggagaaa gccttactta gattaagatt attctgtttc
    36061 tttttgcaga agagactttc ttgccaccga aattaatttt cttcagagag gtgttctcta
    36121 cataagaggt caaaaaagtg tacaatagca ttattaaaga gattatttcc catgtaacca
    36181 attgataaac attgattttc tat
//
LOCUS       DAPGRN010000041        35042 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPGRN010000041 DAPGRN010000000
VERSION     DAPGRN010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.041"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..661
                     /locus_tag="RHW33_002171"
     CDS             65..661
                     /locus_tag="RHW33_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1645669.1"
                     /translation="MKKALLAAALVMASGSALAVDGGHIDFNGMVQSGTCKVGVVDTG
                     MHSVTTDGVVTLDTANVTDTFAEVSATAVGLLPKEFMISVECDPGAPKNAELTMGSAS
                     YANTSGTLNNNMNITVNGIAPAQNVNIAVHNMKNKAGAAEIKQVHMNNSSEVQELTLD
                     AEGKGQYVFNASYVKAPNSPAVTAGHVTTNALYTVAYK"
     gene            748..1473
                     /locus_tag="RHW33_002172"
     CDS             748..1473
                     /locus_tag="RHW33_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV1645670.1"
                     /translation="MFPLVKKTVSALFVSTLLASAPAFADIIISGTRIIYNADKKDVN
                     VRLENKGNRPLLIQNWLDTGDDNADPSQIKVPFTATPPVSRVEPKRGQTVKVMYTGAT
                     ALPKDRESVYWFNVLEVPPKPKDAEADKNLLQLAFRTRIKLFYRPSGLQGEPAEAPAK
                     ITWKLNNAQLQANNPTPYYVSFNEVKLESGGKTYNVNSSMVAPFSQASFGVTGLPGSV
                     SSGKVVFKAINDFGGNIDGSATF"
     gene            1515..4115
                     /locus_tag="RHW33_002173"
     CDS             1515..4115
                     /locus_tag="RHW33_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HDV1645671.1"
                     /translation="MYQFTHQKSRIPKKTLLAACCALFYSGNSSAADTVEYDSSFLMG
                     TGASTIDVKRYAQGNPTPPGLYNVRVFVNGLATSSLEIPFVDIGENSAAACLTRKNLA
                     QLHIKQPEQPVTLLAREGEEEDCLDLAKSYEKADVRFDSSDQFLDLTIPQAYVLKNYG
                     GYVDPSLWESGINAATLAYTLNAYHTSSDNNNSDSVYGAFNSGINLGAWHFRARGNYN
                     WTTDNGSDFDFQDRYLQRDIPAIRSQIIMGDAYTTGETFDSVNVRGVRLYSDSRMLPS
                     ALASYAPTIRGVANSNAKVTVTQNGYKIYETTVPPGEFVIDDLSPSGFGSELVITIEE
                     ADGSKRTFTQPFSSVVQMQRPGVGRWDFSAGKVIDDNLRSEPNMGQASYYYGLNNLFT
                     GYTGIQFTDNNYLAGLLGVGINTSIGAFAVDVTHSRAEIPDDKTYQGQSYRVTWNKLF
                     QDTGTSFNLAAYRYSTQDYLGLHDALVLIDDAKHLSADEDKNTMQTYSRMKNQFTVSI
                     NQPLNIAYEDYGSLFISGSWTDYWAANNSRTEYNVGYSKSVSWGSFSVNLQRSWNEDG
                     EKDDAMYVSVSVPIENILGGKRKSSGFRNLNTQLNTDFDGSHQLNVNSSGNTENNLVN
                     YSVNAGYSLDKNAGDLASVGGYLNYESGLGGISASASATSDNSQQYSISTDGGFVLHS
                     GGLTFTNNSFSSNDTLVLINAPGAKGARINNSNNEIDRWGYAVTSSVSPYRENRVGLN
                     IETLENDVELKSTSAITVPRSGSVVLTRFETDEGRSAVLNITAANGKSIPFAAEVYQD
                     EVMIGSMGQGGQAFVRGINDSGELIVRWYENNQTIDCKLHYQFPAQPQTLGSSNTLLL
                     NNLTCQVANH"
     gene            4129..4677
                     /locus_tag="RHW33_002174"
     CDS             4129..4677
                     /locus_tag="RHW33_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1645672.1"
                     /translation="MKRILLTSALIGLGLPAVEAATTLDVDFTATVLATTCTITIVED
                     GGPAVTGSNDEYSLTIPDVGLDKVVTGDVAAQANFKLKAKDCSNGYSKIFTTLTGTTI
                     SGKLIVNEASSGATGVGMGIKRRDTADSTFFTPNNTDKFEWSTDEKANGVPLTVALRE
                     TTAGAGRTGAFQAKATFNFTYE"
     gene            4692..5303
                     /locus_tag="RHW33_002175"
     CDS             4692..5303
                     /locus_tag="RHW33_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1645673.1"
                     /translation="MKLKVIATLIATVAVGASVSNNFTYASTTSSSLTVNSNLTMGTC
                     SAKVMDTSDNVINTVDIGNVYISELAAKSKVKPFKIRFSDCAGLPGKSAQLVLSPRNI
                     GCAGGMSNTAGFANKLNSTTEGGSSRAAVEVWTTTTPGGNDSQQFSCVQRNVINVDLS
                     SASTTQPYDFDLSARMIIADGRTVNDVLPGSFLSPTTFTITYQ"
     gene            5324..5914
                     /locus_tag="RHW33_002176"
     CDS             5324..5914
                     /locus_tag="RHW33_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1645674.1"
                     /translation="MLCRHHKLVRFLGLATALITPFGYAGQDVDLTAQIVASTCQVEV
                     SNNGVVDLGTVTLDYFADNVTPTTDYAGGKTFNVNVVSCDNIQTTQSQMKLDFQPQAG
                     ALAQANNQIFSNEYEQQATGAKNVGIVIFSAQPNQQTFNVRGTDGSSTAIYSVAPGNA
                     VPSTWTFYSRMQRVNNALPPESGMVRSQVIVNVSYE"
     gene            5926..7035
                     /locus_tag="RHW33_002177"
     CDS             5926..7035
                     /locus_tag="RHW33_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1645675.1"
                     /translation="MQRKGNRLLIQLCSAILLFFTSSWYALANECYIESTAEGDYHMK
                     ISSTQLSLVSQMVEAPQEIAAATWDVNIQLRGDAIGCKSLGDSKAVHFLNTADPSLIS
                     TYTTANGAALLKTTVPGIVYSVELLCINCGAADELDLWLPAQSGADNFIPSTQTKWAY
                     EYSDTSWYLRFRLFITPEFKPKTGVSSGTTIAGKIASWYIGTNNQPWINFYIDDDSLK
                     FFVDEPTCSTVALAQDLGNVSGNQVTLGNSYVSEVKNGLTREIPFSIRADYCYASKIT
                     VKLKAANKPSDGTLVGKTTGSASGVAVKVHSTYENSKVLLKADGSNTVDYNFAVWSNN
                     LLNLPFTAQLVPDGSGNAVGVGTFSGNATFSFTYE"
     gene            7171..7965
                     /gene="panB"
                     /locus_tag="RHW33_002178"
     CDS             7171..7965
                     /gene="panB"
                     /locus_tag="RHW33_002178"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV1645676.1"
                     /translation="MKPTTIASLQKCKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALVIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7977..8828
                     /gene="panC"
                     /locus_tag="RHW33_002179"
     CDS             7977..8828
                     /gene="panC"
                     /locus_tag="RHW33_002179"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV1645677.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9202..10074)
                     /gene="rpnC"
                     /locus_tag="RHW33_002180"
     CDS             complement(9202..10074)
                     /gene="rpnC"
                     /locus_tag="RHW33_002180"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV1645678.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGETTPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEERGRQEERQEFVLRLLNKGMSREDVAE
                     MANLPLAEIDKLIN"
     gene            10348..10728
                     /gene="panD"
                     /locus_tag="RHW33_002181"
     CDS             10348..10728
                     /gene="panD"
                     /locus_tag="RHW33_002181"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV1645679.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10732..11961)
                     /locus_tag="RHW33_002182"
     CDS             complement(10732..11961)
                     /locus_tag="RHW33_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV1645680.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQTIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     EPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12025..12465)
                     /locus_tag="RHW33_002183"
     CDS             complement(12025..12465)
                     /locus_tag="RHW33_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV1645681.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVVFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12570..13340)
                     /locus_tag="RHW33_002184"
     CDS             complement(12570..13340)
                     /locus_tag="RHW33_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1645682.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13337..14263)
                     /locus_tag="RHW33_002185"
     CDS             complement(13337..14263)
                     /locus_tag="RHW33_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1645683.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14372..15034
                     /gene="can"
                     /locus_tag="RHW33_002186"
     CDS             14372..15034
                     /gene="can"
                     /locus_tag="RHW33_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV1645684.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15075..15611)
                     /gene="hpt"
                     /locus_tag="RHW33_002187"
     CDS             complement(15075..15611)
                     /gene="hpt"
                     /locus_tag="RHW33_002187"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV1645685.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15817..18207
                     /gene="gcd"
                     /locus_tag="RHW33_002188"
     CDS             15817..18207
                     /gene="gcd"
                     /locus_tag="RHW33_002188"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV1645686.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18409..19959)
                     /gene="cueO"
                     /locus_tag="RHW33_002189"
     CDS             complement(18409..19959)
                     /gene="cueO"
                     /locus_tag="RHW33_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV1645687.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20125..20472
                     /locus_tag="RHW33_002190"
     CDS             20125..20472
                     /locus_tag="RHW33_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV1645688.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20578..21444
                     /gene="speE"
                     /locus_tag="RHW33_002191"
     CDS             20578..21444
                     /gene="speE"
                     /locus_tag="RHW33_002191"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV1645689.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVDF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21460..22254
                     /gene="speD"
                     /locus_tag="RHW33_002192"
     CDS             21460..22254
                     /gene="speD"
                     /locus_tag="RHW33_002192"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV1645690.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22292..22654)
                     /locus_tag="RHW33_002193"
     CDS             complement(22292..22654)
                     /locus_tag="RHW33_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV1645691.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22829..25426)
                     /gene="acnB"
                     /locus_tag="RHW33_002194"
     CDS             complement(22829..25426)
                     /gene="acnB"
                     /locus_tag="RHW33_002194"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV1645692.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25781..27634
                     /locus_tag="RHW33_002195"
     CDS             25781..27634
                     /locus_tag="RHW33_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV1645693.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPLSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTVPVIPSN
                     TVSAANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDNYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVHEKMQTNPQNQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTARLNNEQRSAVRERLSERGARRLER"
     gene            complement(27705..29129)
                     /gene="lpdA"
                     /locus_tag="RHW33_002196"
     CDS             complement(27705..29129)
                     /gene="lpdA"
                     /locus_tag="RHW33_002196"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV1645694.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29337..31229)
                     /gene="aceF"
                     /locus_tag="RHW33_002197"
     CDS             complement(29337..31229)
                     /gene="aceF"
                     /locus_tag="RHW33_002197"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV1645695.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31244..33907)
                     /gene="aceE"
                     /locus_tag="RHW33_002198"
     CDS             complement(31244..33907)
                     /gene="aceE"
                     /locus_tag="RHW33_002198"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV1645696.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34068..34832)
                     /gene="pdhR"
                     /locus_tag="RHW33_002199"
     CDS             complement(34068..34832)
                     /gene="pdhR"
                     /locus_tag="RHW33_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV1645697.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8624 a         9255 c         8578 g         8585 t
ORIGIN      
        1 aaaaactttg agtaatatcc gctcgcaata ttaatttcat ataatttttg taaggatgca
       61 tgtaatgaaa aaagcacttc tcgcagccgc tctggttatg gcttctggtt ccgccctggc
      121 tgtagatggt ggtcatatcg actttaacgg tatggtacag tccggtacct gtaaagtggg
      181 tgtggtagat actggtatgc acagcgttac cactgatggc gtggttaccc tggatactgc
      241 gaatgttact gatacttttg ctgaagttag cgcaactgct gtcggtttac tgccgaaaga
      301 gttcatgatt tctgttgagt gtgatccagg tgctccgaag aatgctgagt taactatggg
      361 ttctgcaagc tacgcgaaca ccagcggtac cctgaataac aatatgaaca tcactgttaa
      421 cggtattgca ccggctcaga acgtaaacat tgcagttcat aacatgaaaa acaaagctgg
      481 cgctgctgaa attaagcagg tccatatgaa caactcttct gaagttcagg aactgacatt
      541 agacgcagaa ggtaaaggcc agtacgtatt taacgcatct tacgttaaag caccgaacag
      601 cccggctgta actgctggtc atgtaaccac taacgcgctg tacaccgttg cttataagta
      661 attgatggtg tgttgagcat aaggtttgcc agcaggcaaa ccttattttc gcgaatttaa
      721 tctttctaca gacagcaagg agtgcttatg tttcccttgg ttaaaaaaac ggttagtgca
      781 ttatttgtga gtactctgtt agccagtgca ccagcattcg ctgacattat tatttccgga
      841 acacgtatca tttataacgc tgataaaaaa gatgttaacg ttcgcctgga aaataaaggg
      901 aatcgtccct tactgattca aaactggttg gatacgggcg atgacaatgc cgatcccagc
      961 cagatcaaag tgccgtttac cgcgaccccg cccgtttcac gcgtagagcc aaaacgcggt
     1021 caaaccgtaa aagtgatgta caccggtgca acagccctgc cgaaagaccg tgaaagcgtc
     1081 tactggttta acgtgctgga agtgccgcct aagccgaaag atgctgaggc tgacaaaaac
     1141 ttgttacagc tggctttccg cacacgcatc aaactctttt atcgcccgag tgggttacag
     1201 ggagaaccgg ctgaagcacc ggcgaaaatc acctggaaat taaacaacgc tcagttacag
     1261 gcaaataacc caacgccata ttacgtgtcc tttaatgagg tcaagctgga aagcggcggc
     1321 aaaacctaca acgtcaattc cagcatggtc gcgccgttca gccaggcaag ctttggcgta
     1381 accggtctgc caggaagcgt ttcatccgga aaagtggtct ttaaagcgat caacgacttt
     1441 ggcggcaaca ttgatggttc cgcaacattc taaaaaataa acggattctt tttaacatcg
     1501 ccaggaatgc aaccttgtac cagtttactc atcaaaaaag ccgtatcccg aaaaaaacgc
     1561 tacttgcggc ctgttgcgcc cttttttaca gcggcaacag ttctgcggcg gacaccgtgg
     1621 aatatgacag ttccttctta atgggaactg gcgcatcaac gattgatgtt aaacgttatg
     1681 ctcaaggcaa cccgacaccg ccgggtctct ataatgtccg cgtgtttgta aacggtctgg
     1741 ctacgtccag cttagaaatt ccgtttgtgg atattggcga aaacagtgcg gcggcctgtc
     1801 ttacccgcaa aaacctggcg cagctacaca ttaagcaacc cgaacagcct gtcactttac
     1861 tcgccaggga aggtgaagaa gaggattgtc tggatctggc aaagtcatac gaaaaggcgg
     1921 atgtgcgctt tgacagcagt gaccagtttc tcgatctgac tatccctcaa gcttatgttc
     1981 tgaaaaacta cggtggttat gtcgacccct cactgtggga gtcagggatt aacgctgcca
     2041 cactggcata taccctgaac gcgtatcaca caagttcaga taacaacaat agtgacagcg
     2101 tctatggcgc gtttaactca ggtatcaact taggtgcctg gcacttccgg gcgcgtggta
     2161 actataactg gacaacagat aacggcagcg atttcgattt ccaggatcgt tacttacaac
     2221 gtgacattcc ggcaatccgg tcacaaataa tcatgggtga cgcctacacc accggtgaaa
     2281 cgtttgactc tgtcaacgtc cgtggtgttc gtctatacag cgatagccgt atgctgcctt
     2341 ccgcgctggc cagttacgct ccgaccatcc gcggtgtggc aaactccaac gccaaagtca
     2401 cggtgacgca gaacggttat aagatctatg aaaccaccgt tccgcccggt gagtttgtta
     2461 tagacgacct cagcccctcc ggctttggta gcgaactggt cattaccatt gaagaagcgg
     2521 atggttccaa acgcaccttt acgcagccct tctcctccgt tgtgcaaatg cagcggcctg
     2581 gcgtaggtcg ttgggatttc agtgcgggta aagtcattga tgacaatctg cgctcagaac
     2641 cgaatatggg gcaagcctct tattactatg gtctgaataa cctcttcacg gggtataccg
     2701 gcattcagtt caccgataac aactatcttg ccggactgtt aggtgtgggt attaacacca
     2761 gtatcggtgc ttttgcggta gacgttaccc actcccgtgc tgagattccg gatgataaaa
     2821 cctatcaggg gcaaagttat cgcgtgacct ggaacaaact tttccaggat accggaacat
     2881 catttaacct cgcggcatac cgctattcca cccaggatta cctgggcctg catgatgcgt
     2941 tagtcctcat tgacgacgcc aaacatttgt ctgccgatga agacaaaaac accatgcaga
     3001 cgtactcacg tatgaaaaac cagtttaccg tgagtattaa ccagccattg aatatcgcct
     3061 atgaagatta cggttcgctg tttatttccg gtagctggac agactactgg gcggctaaca
     3121 atagccgcac tgaatataat gttggttaca gtaagagcgt ttcctggggc agcttcagcg
     3181 tcaacctgca acgtagctgg aatgaagacg gcgagaaaga tgacgcgatg tacgtcagtg
     3241 ttagcgtacc tattgagaat attttaggtg gcaaacgtaa gtcttctggt ttccgcaatt
     3301 taaatactca gctcaatacc gatttcgatg gttcacatca gttgaatgtt aacagttccg
     3361 gtaacactga aaacaatctg gtgaactaca gtgtcaacgc aggttatagc ctcgataaaa
     3421 acgccggcga tttagcctct gttggtggtt atctcaacta tgaatcaggg ttaggcggta
     3481 tttccgcttc ggcctcggcc acttcggata acagccaaca gtactccatc tccaccgatg
     3541 gcggctttgt gttacacagt ggtggtttga cattcactaa caacagcttc agcagtaacg
     3601 atacgctggt gttaatcaac gccccaggtg ctaaaggcgc acgaatcaat aacagtaata
     3661 acgaaatcga tcgctgggga tatgccgtaa cgtcctctgt cagcccctac cgtgaaaacc
     3721 gggtaggtct gaacattgaa acgctggaaa acgatgttga actgaaaagt accagcgcaa
     3781 tcaccgtacc acgtagtggc tccgttgttt tgacccgatt cgaaactgac gaggggcgtt
     3841 ctgccgtgct gaatattacc gctgccaatg gcaaatccat tccgtttgct gcggaggttt
     3901 atcaggatga agtgatgatc ggcagcatgg gtcagggtgg tcaggcattt gtgcgcggta
     3961 ttaacgatag cggggaatta atcgtgcgtt ggtatgaaaa caaccaaacc attgactgta
     4021 agttgcacta ccagttcccg gcccaaccac aaactctggg aagcagcaat accttattac
     4081 ttaacaatct tacctgtcag gtagcaaatc actaatatgg aaaattctat gaaaaggata
     4141 ctactaacat ccgcgttaat aggtctgggt ttaccggcgg ttgaagctgc aacaactctt
     4201 gatgtcgatt ttacggccac cgtattagcc accacctgca cgataactat agtggaagat
     4261 ggtggtccag cagtaaccgg tagtaacgat gaatacagcc tgacaattcc tgatgttggc
     4321 ctggataaag ttgtcactgg agatgttgca gctcaggcaa actttaaatt aaaggccaaa
     4381 gattgcagca atggttactc aaagattttt accacactta caggaacaac gatttccggt
     4441 aagttgattg ttaatgaagc aagctccggc gctacgggtg tgggtatggg gattaagcgt
     4501 agagataccg cggactcaac tttctttacg ccaaataata ctgacaaatt tgaatggtct
     4561 accgatgaga aggctaacgg tgtgcctctg accgtagcac tgcgcgaaac aacggcggga
     4621 gcaggacgca ccggtgcgtt tcaggcaaaa gccaccttta acttcaccta cgaataacag
     4681 ggacagccac catgaaatta aaagttatcg ctacactgat tgctactgtt gccgtgggtg
     4741 ccagcgttag caacaacttc acttatgcga gtacaacatc ctcctcttta accgtaaaca
     4801 gtaacctgac tatgggtacc tgtagtgcga aggtaatgga taccagtgat aacgtgatca
     4861 ataccgttga cattggcaat gtctatattt ctgaactggc agccaaaagc aaagtaaaac
     4921 cttttaaaat ccgctttagt gattgtgccg gccttccagg aaaaagtgct caactcgtat
     4981 tgtcgcccag aaatatcggt tgcgctggcg gcatgtctaa cactgccggc tttgcaaata
     5041 aacttaactc caccaccgaa ggtggttcaa gcagagcagc ggtcgaagta tggacgacta
     5101 caaccccagg gggaaatgac agccagcaat tcagttgtgt tcagcgaaac gtcattaatg
     5161 tcgatctttc tagtgccagc actacacagc catatgattt cgatttaagc gcacggatga
     5221 ttatcgcaga tgggagaacg gttaatgatg tattaccggg tagtttcctc tcccctacga
     5281 ctttcacaat cacttatcag taatagcgga taaggatatt agaatgctat gcaggcatca
     5341 taaattggta cgttttttgg ggctggcaac agcccttatt acgccttttg gctatgctgg
     5401 tcaggatgtg gatctcaccg cgcagattgt ggccagcacc tgtcaggtag aagtcagtaa
     5461 taatggcgtg gtagatctcg gcactgtgac gctggactat tttgctgaca atgtgacccc
     5521 aacgacggat tacgcgggtg gcaaaacgtt taatgttaat gtggtctcct gcgacaacat
     5581 tcaaacgaca cagtcgcaaa tgaaattaga ttttcagccg caggcgggag cgcttgcgca
     5641 ggcgaataat caaatattta gtaatgaata tgaacagcag gctaccgggg caaaaaacgt
     5701 cggcattgtg atattttcgg cccagccgaa tcagcagaca tttaatgtgc gcggaacgga
     5761 tggttcctca acagcaattt attcggtcgc gcctggcaat gcagttccct caacctggac
     5821 cttttattcc cgtatgcagc gagtgaataa tgcccttcct cccgaatcag ggatggtgag
     5881 aagtcaggtt attgttaacg tatcttacga ataataagga taattatgca aaggaaaggc
     5941 aatagactgt tgattcagtt atgcagtgcg atactgctat tttttacatc gtcctggtat
     6001 gcattggcga atgaatgtta tatagagagc actgctgaag gggactatca catgaagata
     6061 agctctactc agcttagtct ggtgtcgcaa atggtcgagg ctccgcaaga aatagccgca
     6121 gccacatggg atgtaaatat tcagctaagg ggcgatgcca tagggtgtaa atctcttggg
     6181 gatagcaagg cagttcactt tcttaataca gctgacccaa gtttaatatc cacgtacacc
     6241 acagcgaatg gtgcagcatt attaaaaaca actgttccgg gcattgtgta ttctgtcgag
     6301 ttattatgca ttaattgtgg tgccgcagat gaacttgatt tatggctacc tgcacaaagt
     6361 ggcgcagata acttcatacc aagcacccag acgaaatggg cctatgaata cagtgataca
     6421 agttggtatt tacgttttcg cttattcata actccagaat ttaaacccaa gactggtgtt
     6481 tccagcggaa caacgatagc aggaaagatt gcgtcatggt atataggtac caataatcag
     6541 ccgtggataa acttttacat tgacgatgac tctttaaagt ttttcgtcga tgaacctacc
     6601 tgttcaacag ttgccctggc tcaagacctg ggtaatgtca gcggcaacca ggtaacgctt
     6661 gggaacagct atgtttcgga agtgaaaaat gggcttacgc gggaaattcc tttttctatc
     6721 cgtgctgatt attgttatgc cagtaagatt acggttaagt tgaaagcggc aaataaaccc
     6781 agcgacggca cactggtagg taaaacaacg ggctctgctt caggtgtggc tgtaaaagtg
     6841 cattcaactt acgaaaatag caaagtctta ttaaaagcag atggtagcaa cacggttgac
     6901 tacaactttg ccgtctggtc aaacaacctg ctgaatttgc cctttacggc acagttggta
     6961 ccggatggta gcggtaatgc tgtcggtgtt ggaacctttt caggtaacgc gaccttctcc
     7021 tttacctacg aataagtaaa acgtcattcc ttcctcatcg caccagagtt ctgctctggt
     7081 gcgaacattt tccccacact aaaaaccatt gcccccctga tttcgactgt tagaatgcga
     7141 tcgatctgac tttagccatc aggatacgtt atgaaaccga ccactattgc ttcattgcag
     7201 aaatgtaagc aggaaaaaaa acgcttcgcc accatcactg cctacgacta cagcttcgcc
     7261 aaactgtttg ctgacgaagg tctgaacgtg atgctggtgg gtgattcgct gggcatgacc
     7321 gttcagggcc acgactccac tctgccagta accgttgccg atatcgccta ccacactgcc
     7381 gctgtacgtc gcggcgcgcc aaactgcctg ctgctggctg acctgccgtt tatggcatat
     7441 gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg tgctaacatg
     7501 gtcaaaattg aaggcggtga gtggctggtc gaaaccgtaa aaatgctgac cgaacgtgcc
     7561 gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt cggtggctac
     7621 aaagttcagg ggcgcggcga tgaagcgagc gatcgactgc ttagcgatgc attagcctta
     7681 gaagccgctg gtgcacagtt gctggtgctg gaatgcgtgc cggttgaact ggcaaaacgt
     7741 attaccgaag cactggtgat cccggttatt ggcattggcg caggcaacgt cactgacggg
     7801 cagatcctcg tgatgcacga tgcgttcggc attaccggcg gccatatccc taaatttgcc
     7861 aaaaatttcc tcgctgaaac gggcgacatc cgcgcggctg tgcggcagta tatggctgaa
     7921 gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt gacgttgtgt
     7981 taattatcga aaccctgcca ctgctgcgcc agcaaattcg ccgcctgcgt atggaaggca
     8041 agcgcgtggc gctggtacct accatgggca acctgcatga tggccacatg aagctggtcg
     8101 acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac ccgatgcagt
     8161 tcgaccgccc ggaagacctg gcacgttatc cacggacctt gcaggaagac tgcgaaaagc
     8221 tgaacaagcg caaagtggat ttagttttcg cgccctcggt aaaagagatc tacccgaacg
     8281 gtactgaaac ccacacttac gttgacgttc ctggcctttc gaccatgctg gaaggcgcca
     8341 gccgtccggg gcattttcgt ggtgtgtcga ctatcgtcag caagctgttc aacctggtcc
     8401 agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgctg atccgcaaaa
     8461 tggttgctga tatgggcttt gatattgaga ttgttggcgt cccgattatg cgcgccaaag
     8521 atggccttgc gctcagttcc cgtaacggtt atctgacggc ggaacaacgt aaaattgcgc
     8581 ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc gaacgggatc
     8641 tcgatgaaat tattgctatt gccgggcaag aactgaatga aaaaggcttc cgcgccgatg
     8701 atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc aaacgagctg
     8761 tgattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaag atggtcgaac
     8821 tggcgtaata cttaactggc gctacggctg atggcgccag ttattaattt gccctacgtc
     8881 atggcgatga cgtctgtaag tgattattca tatcgctggc aatttagtta gagttaatta
     8941 gcttatcaat ctctgtaaga ggtaaagttg tcacctctgc aacgtcttcc cgagacatcc
     9001 ctttacttag aaaacgctgg gcgaattcct gtcttacctt ctggagttcc tcttgatatc
     9061 ccttctgaaa tcccttctct tcaaaccact gcgccaacgt ctgcctggtt tctctccgtg
     9121 tctttatttc cataggttcc cttaattatc tggtcagtta acaataacaa ttctctggct
     9181 ataactgatg atgcagacaa cttagttaat cagcttatca atctcggcaa ggggtaaatt
     9241 tgccatctct gcaacgtctt cccgagacat tcctttattc agaagacgca ggacgaattc
     9301 ttgcctttcc tcctgtcttc ccctctcttc aaaccactgc gccagcgtca tcatagactc
     9361 ccctcccgtt tccctgtctc tcagcacacc ataaaacaaa tccgcttgtt cagtatgacc
     9421 gcgttgcagc atatagtttt gcatggcaac taactgactt ccgctagtgt acccttcgtc
     9481 aatcagcgtg accagttgct ccagcaataa cattaagtcg cgctggcgaa tatgcttttg
     9541 cagtagttcg agaatcgcaa tccgccggtg ctgcatgatt tcgtcatccg gtgtgatagt
     9601 gatatccacc agcggaaaag gactgttata gacgcgtcgc gccagctccg gcgagtaaaa
     9661 catatcaaac cagcacattg agagcggata aggtgtggtc tcgccctgat aaaacagtat
     9721 cggcaccacc agcggcagct tatcgtgatc cgcctccaga tgccggtgca tggcagcaat
     9781 agaataacgc atcatgcgaa aggccatttt cttatccggc ttgctttggt gttcaatcac
     9841 aacatgcaga taaccgggat tgccctgcat ttgcacggaa tagagcacgt ccgtgctgtg
     9901 tcctttcagg ctctcttcaa tgaaactccc cgactctaaa tgaagcgtgt tgaggtcaca
     9961 aagttctcgt aattccactg gcaaatgtat ctccagaaag tcgcgagccg tctccgcgtg
    10021 cattaaaaat tgtttaaaca ctgcatcatg cggtgtggta ctcggtgcat ccatcttccc
    10081 tgccttccgt attgtcttgc gtgaaggcag tatccagtca aggtgtacca tcgtcagcca
    10141 cctctttagt gtttaccgag cagcgttcag agaaatttca attcgttaca gccgttacat
    10201 ggcacgcttg ggcgcggtac gcaaactgct gatgcaaatg ccgtcagatg agtagacact
    10261 aaacaaaaat cgggcaatac tgcgtgagaa tttcctcaaa gcaggcaccg cctgcttcgt
    10321 taacgacagg gtagaaaggt agaagttatg attcgcacga tgctgcaggg caaactccac
    10381 cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt cttgcgccat tgaccaggat
    10441 tttcttgacg cagccggtat tctcgaaaac gaagccattg atatctggaa tgtcaccaac
    10501 ggcaagcgtt tctccactta tgccatcgcg gcagaacgcg gttcgagaat tatttctgtt
    10561 aacggtgcgg cggcccactg cgccagtgtc ggcgatattg tcatcatcgc cagcttcgtt
    10621 accatgccag atgaagaagc tcgcacctgg cgacccaacg tcgcctattt tgaaggcgac
    10681 aatgaaatga aacgtaccgc gaaagcgatt ccggtacagg ttgcttgatt gttatccctg
    10741 cggctggtta ctcaccagcc gcgacatcgt ctccagcgaa tccgttctta agatataaag
    10801 tcgttttagt aacaacggat tatcccccgg ttttactttg cctttcatgg ttgtcaccgc
    10861 caggtgaaat ccggcatcgt ttgctgcctt cacggctttg tcattaaagc cgccaaacgg
    10921 atacgaaaga taccagacat gcggattaaa ttgcgccaga gcgcggcgtg aacgtgcaaa
    10981 atcaaacaga atattgtgct cactacggct cagtaatatg ggtcggcgat aaccatctac
    11041 ccgatgcaaa aaatgggtat gtgactggaa atcaaataca tcgcgaattt cgttaagctc
    11101 agaaacgctc ataaattgca gcgattttgg ttcccacttc tgcgggtgac gtttgatgcg
    11161 cgaggtaaca ataaacgccg tcgccttcat gccatattgt ttcaacacag gatacgcata
    11221 gcggctcacc gacttgaggc catcatcaaa ggtaatcacc accgctcgcg cagggagatt
    11281 gatcttattc ttcacgtaac cttccagctg caccatgctc agtgtcgcgt atcccctgtc
    11341 gcgcagccag gccatctggt tattgaaagc gcgtaccgag gtggtcgtcg aagtatggcg
    11401 aaaacgggtg ttttcttcgt cgcgcagaat atggtgataa gttagcaccg gaaggccatt
    11461 atcgggttgg gcatccagcg cgctgatata ggccagtcga tcgccaatac ggacctgata
    11521 ccaggtttga tttaacctgt ctttcagttt atgcaaaatc gggtagcgca aattgtccgc
    11581 cagtacccca aatggcgcac tgcccacact cggcgcgtta tagaccggcg tatctttcca
    11641 ggtaactaag ttctgattac tcagcggctt gttgaggtca cccaaaccgt cttcaacttt
    11701 ttgtcgcccc tgaaccggct caagatgacc tttatcgata aaccctttac caaagccaaa
    11761 attaaatgcg taataacttg cggcagtggg ctccacggca atggtttgtc cggcccgaat
    11821 attccccacg gtcaccatct tgtcaccaat ttgcgcccag accgcagcat tttcgatggt
    11881 ttgcatataa cgggcaggta acgcggcact gacacttgcg gtaaacagca tcagcaggag
    11941 aataacagct tgtttgtaca tgaaaaaata accgagggtg aaacaacgct tttattgtag
    12001 caaaagcgct ataagaatcg tgggttaata ctcatacaaa ttatgtttga ggacgaaagg
    12061 cggatttttt tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat
    12121 acgctcgcga aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa
    12181 cggtaacgtg acaccagaaa tcacttcgca acgggagtgt ttgtggctta ataatgaagc
    12241 cacgcggtac ggtggcgctc cggctatatc ggttaagaag acgacaccct caccagagtc
    12301 cgcttcatgc agagcatcgc acatcatgcg gctgagcata ttagaactta atccgcgcca
    12361 gaaattcacg gcccggcact gaagaagcgc cccatgtttt ttctccagcg catccagtat
    12421 ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag
    12481 ccgaaaatca gtctaccctg accattgttg ataaagggtg atagccgtca aaactcctct
    12541 cccccaaatc cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc
    12601 agcgaccaac agatcaaata aaacgccaca ataaagacca ccagtacgcc aaaggtagtg
    12661 accagcggaa catcattgat accgaggaag ccgtagcgga aaccactgat catataaacg
    12721 attgggttca ggtgcgacag cccttgccag aacggcggca acaaagtcag tgagtaaaac
    12781 accccgccca aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg
    12841 aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc
    12901 accagggtta aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa
    12961 attgccgtca ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg
    13021 gcaataatga cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaat
    13081 ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcgt tggtgatcac cgacatcatg
    13141 atcagccccg gtacgatgaa ctgcatgtag ctgaagccat gcatatcgcc aatgcgcgaa
    13201 ccaatcaggt taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc
    13261 gtctgcaccc agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc
    13321 acccagtaaa gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca
    13381 gctcttccag acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta
    13441 actgcgtaaa tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg
    13501 tatcgaccag tcgatactga tagccatcga gcttcggtaa cggacttttc ggtgcgagat
    13561 cgagaataaa ggtttccgat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca
    13621 ccagctcacc gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt
    13681 agtgtgtggt gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca
    13741 ttgagcggcg aagttcaata tccacgcctg cagtcggctc gtcgagaatc agtagtttag
    13801 gttcatgcat taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatgc
    13861 gcgcacgttc gttgcgtttt ccccataaat cgagctgctt aagatatttt tcgctgcgga
    13921 tgtacgcctc tttgcgctcc acaccgtagt accctgcctg attcaccaca atttgctgca
    13981 cggtttcaaa cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt
    14041 tcacgacatc tttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta
    14101 ccagagagct gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg
    14161 cataaaaatc acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg
    14221 gataggtttt tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt
    14281 acgttcttac actttatctg tggtttaaat cgtcccggag ttgccctata ttagccaaac
    14341 gtaattattt ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa
    14401 caatgcacta tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc
    14461 acaagcgcaa aaaccgcgct ttctatggat tggatgttcc gacagtcgcg ttcctgcaga
    14521 acgtttaacc ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt
    14581 cattcacacc gacctgaact gcctttccgt ggttcagtat gcagtggatg tactcgaagt
    14641 tgaacacatt attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa
    14701 cccggaactg gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca
    14761 tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa
    14821 cgtcatggaa caggtgtata acctgggcca ctccaccatt atgcaatcag cgtggaaacg
    14881 cgggcagaaa gttaccattc acggctgggc ctacggtatt cacgacggct tgctgcgtga
    14941 tctggatgtt actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc
    15001 caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc
    15061 cggcaacttc acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg
    15121 taacgctgtg cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg
    15181 ataaattcta ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc
    15241 gccagcgact tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc
    15301 gagtcgatga tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct
    15361 ttgaggattt tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata
    15421 aagtcgactt catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag
    15481 ccacgcagca gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc
    15541 tgacgaccca gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca
    15601 gtatgtttca tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt
    15661 tggcaccgca acaaagcgca agacacgcag tataccagca aaacgattca ttcgcggaat
    15721 ctgttaataa agctcaaatt gtgatgacga tcacacatgt taaaacctat aattaattgc
    15781 tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg
    15841 cgacgattac tcgtcacgct aacagccctt tttgcagcgc tttgcgggct gtatctactc
    15901 attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt
    15961 gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac
    16021 gccgccctgc tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg
    16081 gcgctgactc cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt
    16141 gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg
    16201 ctgattagcg gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc
    16261 accttaagcg ccgatgccac acctgcggaa gctatctcac ccgttgccga tcaggactgg
    16321 cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc
    16381 gataacgtcc ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg
    16441 aacgatccgg gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac
    16501 ctgtgtaccg ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg
    16561 cattacgatc ctgagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc
    16621 tcttatcatg aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt
    16681 cgtatcattc ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa
    16741 ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc
    16801 aaaccgggtc tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg
    16861 gcaggttcag ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt
    16921 gatgtcaaca ccggggagct gctgtgggct tttgatccgg gcgcgaaaga tccgaacgca
    16981 atcccgtctg acgaacacac ctttaccttt aactcgccaa actcgtgggc accagcggcc
    17041 tatgacgcga agctggatct ggtgtatctg ccgatgggcg tgaccacgcc ggatatctgg
    17101 ggcggtaacc gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc
    17161 acaaccggga aattggcgtg gagctaccag accgttcacc acgacctgtg ggatatggat
    17221 cttccggcac agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt
    17281 tacgctccgg cgaaaaccgg caacattttt gtgctcgatc gtcgtaatgg cgaactggtg
    17341 gttccggcac cggaaaaacc ggttccccaa ggtgctgcca aaggcgatta cgttacccct
    17401 actcaaccat tctcggaact gagcttccgt ccgacgaaag atttgagcgg tgcggatatg
    17461 tggggagcca ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat
    17521 gaaggcattt tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga
    17581 atgtttgaat ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca
    17641 atggcactgc cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag
    17701 ccgaaagatg ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg
    17761 tatggtgtca cgctcaaccc gttcctttca ccatttggtc tgccatgtaa acagccagca
    17821 tggggttata tctcggcgct ggatctgaaa actaatgaag tggtgtggaa gaaacgtatt
    17881 ggtacgccgc aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt
    17941 atgccgatgc tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg
    18001 gcagataact acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt
    18061 ttaccagcgg gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg
    18121 gtgatctccg caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct
    18181 tatgcgctgc cggatgatgt gaagtaagac ttgctcagat tgctgacaac gtgcgcgttg
    18241 ttcatgccgg atgcggcgtg aacgccttat ccagcctaca aaattgtgca aaatcaatgg
    18301 attgcacagg tagcgtaggc ctgataagcg tagcgcatca ggcagatttg cgtttgtcat
    18361 cagtttaatg cccggagaga tccgggcata ttcccgaata cggtcttttt ataccgtaaa
    18421 ccctaacatc atccccgtat cttcatgctc cagcagatgg cagtgcgcca tataagcatg
    18481 ttctttcggt gcgtcgtgat taaacttcac cagcacttca ctgacattgc cttctacctt
    18541 aacggtatct ttccagcccg cgcgatgagc cgctggcggt ttgccatttt ctgacaagat
    18601 acggaactgc gtgccgtgaa tatggaacgg atgcagcatc atgtcgccca cgccagagat
    18661 aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc ggcttattca tatcaaacgc
    18721 ctgaccgttg attttattgg catggtggaa atcgaacttc ccgctgtggt tcatatgatt
    18781 catattgccg tgccccatat ggcccatcat ctggctgtga tccattccgg ccatcgcctg
    18841 atcgccatat ttctccatta gcatctgcat ccccatcata tcgagcatcg ggtccataga
    18901 aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt aacgcaggca ggctacttaa
    18961 tgtgtctggc aaagcaccgg aggcactaat agcaatcggc tgaatccgca ttaccggatg
    19021 cggcttgtca aacggtgcaa tcgccatccc catctggctg accggcagcg tcaccaggtc
    19081 aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa cgctcgccca tcagcaccgg
    19141 cagctcgttc accttcaccg gttcaggtag cagaccaccg tcgctggcaa tcacataaag
    19201 cgggcgattg tcgctggtgg cgaaattgag cgagcgggca ttacagccat tgagcaaacg
    19261 caggcgcagc caaccacgcg gggcagcgtg ttgcgggtag attgcaccgt tggtcagcaa
    19321 cgtatcgcca aaccagccca cggcggcggt catcacatcc agttgataat caatctgccc
    19381 gtcggcgcta aatttcttat cctgaacgat caccggaaca tcatcgatac cccactgttt
    19441 tggcagcatt aatttcagga tctcgtcatc ttcaatcacc accagaccag ccagccccat
    19501 cgccacctgt cgcccggtct tgccatgttg atgtggatgg aaccagcagg tagcggcagg
    19561 ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc ggaataattc cctgtgggcc
    19621 gccgtcgact tcacccggta cttccagccc gtgccagtgc aacgtcgtct cttccgtcag
    19681 ttggttatag atatcaaccg ttaccgcttt gccgcgctgt aatttcaccg ccggccccag
    19741 cagattgccg ttatagcccc aggtagttgc agttttctcg ccaaaggtag actgacctgc
    19801 gccaatagtt aactgaatgc gattacgggc atcggtcgtg agcaaatcag ggatcggtaa
    19861 cgttgggcgt tccgccgcaa agactgcacg gctccacagt ggcaaggctg aagccacacc
    19921 cagtgcgacg gaatatttca agaaatcacg acgttgcata gttatttcct tattcttaag
    19981 caggcgaaac aaaatcaaac gttgagcata gtccttcccc ttacgggaag gtcaagccgc
    20041 agacacaatt taatcgacaa taatggtcgt cgcctcgctc gcagtgtgct aacgtttatc
    20101 ttctttaagc ccctggtaga agcaatgaag acgtttttca gaacagtgtt attcggcagc
    20161 ctgatggccg tttgcgcaaa cagttacgcg ctcagcgagt ctgaagccga agatatggcc
    20221 gatttaacgg cagtttttgt ctttctgaag aacgattgtg gttaccagaa cttacctaac
    20281 gggcaaattc gtcgcgcact ggtctttttc gcccagcaaa accagtggga tctcagtaac
    20341 tacgacacct tcgacatgaa agccctcggt gaagacagct accgcgatct cagcggcatt
    20401 ggcattcccg tcgctaaaaa atgcaaagcc ctggcccgcg attccttaag cctgcttgcc
    20461 tacgtcaaat aatccctgat acctttttgt agaaatattg accgtgcatc cgcggtcaat
    20521 gttagctatt atgttgcgcc ctttttttac gggtgttaac aaaggaggta tcaacccatg
    20581 gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt ttgggcagta ctttgcggta
    20641 gataacgttc tgtatcatga aaagaccgat caccaggatc tgatcatttt tgagaacgct
    20701 gcatttggtc gcgtaatggc gctggatggc gtagtacaaa ccaccgagcg cgacgagttt
    20761 atctatcatg agatgatgac ccatgttccg ctactggccc acggtcacgc gaaacatgtg
    20821 ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag taacccgaca taaaaacgtt
    20881 gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtgt cgttctgccg tcagtatcta
    20941 cccaaccata acgccggtag ctacgacgat ccgcgcttta agctggtgat cgacgatggc
    21001 gtcaatttcg ttaatcaaac cagccagacc tttgatgtca ttatctccga ctgcaccgat
    21061 cctatcggtc ccggcgaaag ccttttcact tcggcatttt atgaaggctg caaacgttgc
    21121 ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct gctttttaca gcaggaagaa
    21181 gccatcgaca gccatcgtaa actcagccat tacttcagcg acgttgactt ttatcaggcg
    21241 gcgatcccga cctattacgg cggtatcatg acttttgcat gggcgacaga taacgacgcc
    21301 ttacgccatc tctcaaccga aattattcag gcgcgttttc tcgcctctgg cctgaaatgc
    21361 cgttattaca atccggcagt ccatacggca gcttttgcct taccccagta tctgcaagac
    21421 gcactggctt cacagccgtc ctaaggagaa gataagaaat tgaaaaaact gaaactgcat
    21481 ggctttaata atctgaccaa aagtctgagt ttttgtattt acgatatctg ctacgccaaa
    21541 actgccgaag agcgcgacgg ttatattgct tatatcgatg aactctataa tgccaaccgt
    21601 ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg ctaatattct taacatcgcc
    21661 cgccaggatt acgaaccaca gggtgccagc gtcactattc tggtgagtga agaaccggtt
    21721 gacccgaaac ttatcgacaa aacagaacac cccggcccac tgccagaaac ggtcgttgcc
    21781 catctcgata aaagtcatat ttgcgtacat acctacccgg aaagtcatcc tgaaggcggt
    21841 ttatgtacct tccgcgccga tattgaagtc tctacctgcg gcgtgatttc tccgctgaag
    21901 gcgctgaatt acctgatcca ccagcttgag tccgatatcg taaccattga ttatcgcgtg
    21961 cgcggtttta cccgcgacat taacggtatg aagcacttta tcgaccatga gattaattcg
    22021 attcagaact ttatgtctga cgatatgaag gcgctgtatg acatggtgga tgtaaacgtc
    22081 tatcaggaaa atatcttcca taccaagatg ttgcttaaag agttcgacct taagcactac
    22141 atgttccaca ccaaaccgga agatttaacc gacagcgagc gccaggaaat taccgctgcg
    22201 ctgtggaaag aaatgcgcga gatttattac gggcgcaata tgccagctgt ttaacggctc
    22261 tggcggagct cccaggctcc gccagattta tttacttctg ctgcacgaaa ttgcggtaag
    22321 ccgccacgac ctgcagaaaa tcctcaacgc cgcatagcga caggctttct tcgtcgtagt
    22381 agttcatccc ctcttccatt tcatcgccag cgaattccag ttgattggcg cgaaccatca
    22441 cctcttcacc gtccatccac agggtgtatt catgccctgc ccgttgccag gaccgttcgc
    22501 tacctttcag tgcgtgcgct gcttgttcca cttcatcaag caaggccagg ttttctttca
    22561 cctcttcatt aaaccagtgc ccgaccactt catgccccat ggacatacgc acctttacca
    22621 ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg ttcctctgct cccggcaatg
    22681 caccaaaatc gcattgcgcc attgatgtaa ttatcgcagc cttaccggag aaaaacagcg
    22741 gaacggcgat ggtttagaaa taaaaaatgc ccgggaatct cattcaccgg gcattatgtc
    22801 gtttatgcgc agcgcgtgcg ctgacttttt aaactgcagt ctggaaaatc acaccatcgg
    22861 ctttttcagt gtactgagaa agctggttga agttcaggta acggtaagta tcaacggcag
    22921 ttttatccac ctgcgccacg taggtctggt actcttccgg cgtcggtagt ttgccaatca
    22981 gcgccgcaac agcagccagt tccgcagaag ccaggaagac attcgcgcca gtccccagac
    23041 ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc accgtctgct acacgcgcct
    23101 ggttacccat acacagggaa cagccaggga tctcgatacg agccccgctc ttaccgaaga
    23161 cgctgtagta gccttcttca gtcaactgtg cggcgtccat acgggttggc ggtgccaccc
    23221 acaggcgggt cggcaactgg cctttatgcg catccagcag tttacccgca gcacggaagt
    23281 gaccgatatt ggtcatgcag gaaccgataa acacttcgtc gatcttctca ccctgtaccg
    23341 cagacagcgg acgcgcgtca tccgggtcgt tcggagcaca caggattggc tctttaatat
    23401 ccgccagatc gatgtcgatc actgccgcgt attccgcatc tgcatcggct tccagcagct
    23461 caggattcgc cagccatttt tccatgccct gaatacgacg ttccagggtg cgacgatcgc
    23521 cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt agagttcagg tattcgatga
    23581 tcggttcttt gttcagcttg atggtacaac cggcagcaga acgctcggcg gacgcatcgg
    23641 tcagttcaaa tgcctgctca actttcagat ccggcagacc ttcaatttcc agaatgcgac
    23701 ctgagaagat gtttttcttg cctttcttct caacggtcag cagaccttgt ttgatcgcat
    23761 acagcggaat ggcgtgtacc agatcgcgca gggtgatgcc cggctgcatt ttgcctttga
    23821 agcgcaccag aacggattcc ggcatatcca gcggcattac gcctgttgcg gctgcaaatg
    23881 ccaccagacc agaacccgcc gggaaagaga taccgatcgg gaaacgggta tgggagtcac
    23941 caccggtacc gacggtatcc ggcagcagca tacggttcag ccaggagtga atgacgccgt
    24001 cacccggacg cagcgacaca ccgccacggt tcataatgaa gtccggcagc gtgtggtgcg
    24061 tgttcacgtc aactggcttc ggatacgccg cggtgtggca gaaagactgc atcaccaggt
    24121 cagccgagaa gcccaggcac gccaggtctt tcagttcatc acgggtcatc gggcctgtgg
    24181 tgtcctgaga gcctacagaa gtcattttcg gttcacagta cgcgcccgga cgaatgcctt
    24241 tcacgccaca ggcacggcct accatttttt gcgccagcga gaagccgcga tcgctctcag
    24301 caacatcttt cgcctgacgg aacacatcac tgtgcggcag accaagtgct tcacgcgctt
    24361 tggtggtcag gccacgcccg ataatcagcg gaatacggcc gccagcacgc acttcatcaa
    24421 tcagcacgtc ggttttcagt tcgaaggtcg ccagcagttc gcctgtttcg tggttacgca
    24481 cttcgccttt gtacggataa acgtcaatta cgtcgcccat gttcaggtta gagacgtcca
    24541 cttcaattgg cagtgccccc gcatcttcca tcgtgttaaa gaagatgggt gcaattttac
    24601 cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg tggaatatca tcgcccataa
    24661 accacagaac ggagttggtg gcggatttac gcgaagaccc cgtacccaca acgtcaccga
    24721 catatgccag cgggaaacct ttctgctgca gagcttcgat ttgcttaatc ggaccaacaa
    24781 cgcctggctg atctggctca ataccttcac gggcgttttt cagcatcgcc agcgcgtgca
    24841 gtgggatatc cgggcgtgac cacgcatccg gtgccggaga aaggtcatcg gtgttggttt
    24901 cgccagtgac tttgaagacg gtaacggtca gtttttcagc cagcgccggg cgattcagga
    24961 accattcggc atccgcccag gactgcatta cctgcttcgc atattcgttg cctgctttcg
    25021 ctttctcttc tacgtcatag aagttatcga acatcagcag tgtgtgagaa agtgctttgg
    25081 cagcgatcgg tgccagtttg gcatcatcca gcgcgtcgat cagcggatga atgttataac
    25141 caccctgcat agtgcccagc agttcaatgg ctttttctgg agtcagcagt ggggatttgg
    25201 cttcgccttt ggcgacagca gccaggaagc ctgctttaac ataggcggct tcatcgacgc
    25261 caggaggaac acggttggtt aacagatcta acaggaattc ttcttcgccc gcgggcgggt
    25321 ttttcagcag ctctacaagt gcggccattt ggtttgcatc caggggtttg ggcgcaatcc
    25381 cctcagcggc acgctcagct acgtgcttac ggtattcttc tagcacgacg gttctcctcg
    25441 ctctcattgt catagtgcgg caggcgattc tcttcacgct cctgtgagac agcagtttgt
    25501 agggtaaatg cccagtaccg caacgggcag aatagcagga ttttgacgat gtgttaatct
    25561 gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta aggatggtct agagattgtt
    25621 tccctccgta aaaacttcgt taattcgcat agtgataatc acctttctca acgaatccca
    25681 agattagtca aaatttaaac taccgcctct ttatactcag atttacagca cctgcgggtg
    25741 gcagttcgcc gaccattgcg atttccttga gatccgaatt atgaaaatga ctttgccgtt
    25801 taaaccccat gtgctggcgc taatttgcag tgccgggctt tgtgccgcct ctgccgggct
    25861 atatataaaa agccgcacag tggaagcgcc tgtggaaccg ctatcgacac aactggctgt
    25921 gtctgacgct gccgcggtta cgcttcctgc aacggtttcc gcgcctcccg taacacccgc
    25981 cgtcgttaaa tccgcattca gcactgcaca aatcgatcaa tgggttgcac ctgtcgcgct
    26041 ataccccgac tctctgcttt cacaagtgtt aatggcatca acctatccgg caaacgttgc
    26101 tcaagcagtg caatggtcac acgataatcc acttaaacaa ggcgatgctg ctattcaggc
    26161 ggtatctgac cagccgtggg acgctagcgt caaatcactg gtggcctttc cacaattgat
    26221 ggcgttgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt ttctggcgca
    26281 gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac aaaccggttc
    26341 gctgaagtca tcaaccgaac agaaagttat taccacaacg aagaaagctg taccggtaaa
    26401 acagacagtc acggttcccg tcataccatc caataccgtt tcagctgcca accctgtcat
    26461 tacagagcct gctacaaccg tcatttccat tgagcccgcc aatcctgatg tggtctatat
    26521 tcccaactac aacccaaccg tggtttacgg gaactgggcc aatactgcgt atccgccggt
    26581 ttatctgcca ccaccagccg gagaaccgtt tgttgacagc tttgtgcgcg gattcggcta
    26641 tagcatgggc gttgctacca cgtacgcact attcagcagc atcgactggg atgacgacga
    26701 tcatgaccat catcatcatg acgatgataa ttatcatcac cacgatggcg gtcatcgtga
    26761 cggtaatggc tggcaacata acggcgacaa catcaatatc gacgtcaaca atttcaaccg
    26821 tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc caaactaccg
    26881 taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa ctgatgtcaa
    26941 cggcggaatg agcgccacgc agctacctgc tccaacacgc gacagccagc gtcaggcggc
    27001 agcaagtcag tttcagcaac gaacacacgc cgcccccgtc attacacgag atacccaacg
    27061 tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg acgacttccg
    27121 cgacttcagc cgtcgccaac cactgaccca gcaacaaaag gacgccgctc gtcagcgtta
    27181 tcagtcagct tctcctgagc agcgccaggc agttcacgag aaaatgcaga ctaacccgca
    27241 gaaccagcag cgaagagagg cagcgcgtga gcgcattcag cccgcctcgc ctgagcagcg
    27301 ccaggcagtc cgcgagaaaa tgcagactaa cccacagatc cagcagcgaa gagacgcagc
    27361 gcgtgagcgt attcagtcag cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca
    27421 gcagcgccca ctgaaccaac agcaacgtga taacgcccgc cagcgtgttc aatcagcatc
    27481 acctgaacaa cgtcaggttt ttcgggagaa agttcaggag agccgcccac aacgtctaaa
    27541 cgacagtaac catactgcca ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct
    27601 ctctgagcgc ggagcaaggc gactggaaag gtaaattaca ggcgtaaaaa aagcggcgtg
    27661 gttagccgct tttttaattg ccagatgtcc cggcaaacga aaaattactt cttcttcgct
    27721 ttcgggttcg gcaggtcagt aatgctacct tcgaacactt ctgccgccag gcccacagac
    27781 tcgtgcagag tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt
    27841 tcgattgcca ggccgatttc acccagcagc tcgccgccgt tagtaccgac aatcgcacca
    27901 ccgatcacac ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg
    27961 gaagcgatag cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct
    28021 ttctctttcg cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg
    28081 atggacggga taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca
    28141 acgtgacctt cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat atcgccgata
    28201 gcaaagatgt gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg
    28261 tcaacttcca caccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc
    28321 gctaccagca cggcgtcgta acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc
    28381 acataaatgc cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg
    28441 aatttcttgc tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata
    28501 acctggtcga acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta aacggtgccc
    28561 atttccagac cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt
    28621 tccagcgcgt cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttga
    28681 atcgggcgag aacccgctgc aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc
    28741 tcaccttcaa cttccagggt gttagccccg gtaaatttac ccagaccgtt gaccactttg
    28801 actttgcggc ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct
    28861 ttccaggtac gaatcttgtc gatatcggtt ttcggttcgc cgaagacgat accgtgttca
    28921 gccagcgctt tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata
    28981 cagccgacgt tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc
    29041 agacctaaat cagcgcaacg gaaggcagcg gagtaacctg cggggcctgc cccaagtacc
    29101 acgacctgag ttttgatttc agtactcatc atgacctcta tatatttatc tccggcgggt
    29161 ctgacgtttt ttttatgacg cccattatcc accgggtcgt tctatccgtc ggtattttac
    29221 aaaattgtta acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc
    29281 tcaatacatt catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac
    29341 atcaccagac ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca
    29401 tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc
    29461 acgaactctt taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc
    29521 acttccggcg cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag
    29581 atggtgaagc aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta
    29641 gaaatagtca tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat
    29701 accggaacaa ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc
    29761 agggtcagac gctgaccgtc ttccgacagc gaactgttga agcgaggcat ctgctcaagc
    29821 gctgcagcaa cggctttcat gatgaagaca actggggtga tcttcacatc cagcttacgt
    29881 ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg
    29941 tcgaagtgag taacatgcgg gatcatcacc cagttacggc tcaggttcgc accagagatt
    30001 ttctggatgc ggcccagttc cacttcttcg atttcaccaa acttgctgaa gtccaccttc
    30061 ggccacggca gcatgcctgg gataccgccg ccagttgccg ccggagctgc ttctgcacgt
    30121 ttgatagctt ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca
    30181 gtgcccttca ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcgga
    30241 gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt tgccttccgc tttcgcagcc
    30301 ggtgctgctg ccggggcttc agcttttgct gccggagccg gcgctgccgc ttcctgtttc
    30361 gcaggagctg ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgagccagtt
    30421 ttcactttat cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccgga
    30481 acttccatag aagctttgtc gccttctacg gtaatcagtg actgttcagc ggcaactttg
    30541 tcgcccactt tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg
    30601 ttaacttctt tcacgccagc tgctggtgca ggggccgctg ccggagccgc ttcctgttta
    30661 gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg
    30721 gtagacactt tgtcacccac gttcactttg atctctttca cggtacctgc aaacggagcc
    30781 ggaacttcca tagaagcctt gtcgccttct acggtgatca gcgactgttc agcttcaact
    30841 ttatcgccaa ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga
    30901 acgttaacgt cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc ttctttcttc
    30961 tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc
    31021 agtgcgccgg tctgggtttt atcgccaaca gagactttga tctctttaac aatacccgcc
    31081 tgcggagacg gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg
    31141 gcttcaactt tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg
    31201 atgtccggta ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt
    31261 taactttatc tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga
    31321 tttcgccacg tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt
    31381 cgaagtggtg acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta
    31441 cgcggtagtc gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt
    31501 cggtagatgc cactgccgga gcgtcgttca tcacctgagc gatatacgga acgcgcggag
    31561 tttccagcgg gtgcagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg
    31621 tgaaggaggt cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg
    31681 ccgcttcacg gacgtgacgc aggatagaac cggagcccag cagctgaact ttacctttgc
    31741 taccttcaat ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg
    31801 gcattgccgg catgtggtag ttttcgttca gcgtggtgat gtagtagtaa acgttctctt
    31861 gtttttcacc gtacatacgc tccagaccgt catgcataat gacagcaact tcgtaagcgt
    31921 aagccgggtc gtaagagata cagttcggaa tagtcagcga ctgaatgtgg ctgtgaccat
    31981 cttcgtgctg cagaccttca ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga
    32041 agccacgcgc ttgctggtcg ccagccgccc agcacagatc gccgatacgc tggaagccga
    32101 acatcgagta atagatgtag aacggaatca tcggcagatt gttggtgctg taagaggtcg
    32161 ccgctgccag ccaggaacaa cctgcgccca gctcgttgat cccttcctgc agaatctgac
    32221 ctttctcgtc ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac
    32281 cgttcgggct gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt
    32341 cgtcggcgat gatcggtacc agacgatctt tgatcgactt gttcttcagc atcacgttca
    32401 gggcacgaac gaaagcgata gtggtagaga tctctttgct ttgctcttcc agcagcgcgc
    32461 cgaagtcttg caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca
    32521 gataaccgtg cagtttctga cgctgagcgt gcagataggt atgctcttca gaaccttccg
    32581 ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt
    32641 cgcggatgtg acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac
    32701 cttcagccgc gtcgcccatg ccgtaacctt taatggtatg agcaaggatt accgtcgctt
    32761 tgcctttggt ttcctgcgct ttcttaaatg cagcgtagat tttcttcgga tcgtgaccac
    32821 cacggttcag tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag
    32881 gatatttacc gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtctggt
    32941 agtcgccgtc aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca
    33001 gcagttcatc ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga
    33061 agataccttc cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct
    33121 gcaggttaca gttgataacg aagaccaggt tatccagttt ttcacgggta gcgatggtga
    33181 tcgcaccttt ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct
    33241 gtttagaggt atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa
    33301 tagcaccaat cggacccaga cccatagata cggtcgggaa ctgccagaat tccggcatca
    33361 gtttcgggtg cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca
    33421 gctgctcctg agtcagacga ccttccagga aagcacgtgc gtatacgccc ggggagatgt
    33481 ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt
    33541 taaagcacac atcataaatg gtcgcggaag actggaagga cgccatgtgg ccgcccagtt
    33601 cgaggtcttt tttcgacgca cgcagaaccg tcatgatggc gttccagcgg atagctgaac
    33661 gaatacggcg ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg
    33721 tgttgatgta gttgctgata ccggtgcctg cggctacgtt gacaccgcct ttgcgggctt
    33781 cagcaagcag ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg
    33841 attcgatcgc ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt
    33901 ctgacatggg ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt
    33961 gaactctcgc cggaaagccc aataagacag gttctacgtt tagttgccgc gcttttatat
    34021 gcgcttgatt tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt
    34081 tgctccagac gacgcagaga acgctcacgg cggctttctt cacggcttct gtcgagcaaa
    34141 atttcttcga taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg
    34201 gccataatcg cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga
    34261 cgcgaataga gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac
    34321 cttagcagat gaagcagaac cacattgtgg gccgcttcgg tgacggcaat ctgatactgg
    34381 agtacggcgt ttgattccgc gtccagatcg ccagactgct gcgccagctc tatggcgtgg
    34441 tggagttcac ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg
    34501 gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat actgtgattc aggatggtcg
    34561 gagagcagct ctaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg
    34621 ccgccaccct ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc
    34681 aaggagggac gggagacgtc aaactgtttt gccagttcgc gttccggtgg gagtttttcg
    34741 cccgggcgga gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag
    34801 agttttggtt ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccgaa
    34861 gtcaattggt cttaccaatt tcatgtctgt gacgctaaag taacaaagta ttcaccttat
    34921 gtccatacag gttttgattg aaatcatgaa actgtgcaca ttttaacaac ttgacatata
    34981 taacgtttca aagttgtaac tatgcacaaa tgtagacttt acgtaggaaa gggatttttt
    35041 aa
//
LOCUS       DAPGRN010000042        34803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPGRN010000042 DAPGRN010000000
VERSION     DAPGRN010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.042"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            420..641
                     /locus_tag="RHW33_002200"
     CDS             420..641
                     /locus_tag="RHW33_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645698.1"
                     /translation="MGLWLKIFKIVVGENENYLKDVMMQLEAKNNEGKSVISQANGNP
                     VFKELFWKAIDEFNFPQEELNRLNQYRSL"
     gene            complement(780..2144)
                     /gene="mnmE"
                     /locus_tag="RHW33_002201"
     CDS             complement(780..2144)
                     /gene="mnmE"
                     /locus_tag="RHW33_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV1645699.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGISEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(2250..3896)
                     /gene="yidC"
                     /locus_tag="RHW33_002202"
     CDS             complement(2250..3896)
                     /gene="yidC"
                     /locus_tag="RHW33_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV1645700.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNADEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3899..4156)
                     /gene="yidD"
                     /locus_tag="RHW33_002203"
     CDS             complement(3899..4156)
                     /gene="yidD"
                     /locus_tag="RHW33_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV1645701.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4120..4479)
                     /gene="rnpA"
                     /locus_tag="RHW33_002204"
     CDS             complement(4120..4479)
                     /gene="rnpA"
                     /locus_tag="RHW33_002204"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV1645702.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRHHCRLARGS"
     gene            complement(4496..4636)
                     /gene="rpmH"
                     /locus_tag="RHW33_002205"
     CDS             complement(4496..4636)
                     /gene="rpmH"
                     /locus_tag="RHW33_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV1645703.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4866..4946)
                     /locus_tag="RHW33_002206"
     CDS             complement(4866..4946)
                     /locus_tag="RHW33_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645704.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            5243..6646
                     /gene="dnaA"
                     /locus_tag="RHW33_002207"
     CDS             5243..6646
                     /gene="dnaA"
                     /locus_tag="RHW33_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV1645705.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6651..7751
                     /gene="dnaN"
                     /locus_tag="RHW33_002208"
     CDS             6651..7751
                     /gene="dnaN"
                     /locus_tag="RHW33_002208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV1645706.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7751..8824
                     /gene="recF"
                     /locus_tag="RHW33_002209"
     CDS             7751..8824
                     /gene="recF"
                     /locus_tag="RHW33_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV1645707.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8853..11267
                     /gene="gyrB"
                     /locus_tag="RHW33_002210"
     CDS             8853..11267
                     /gene="gyrB"
                     /locus_tag="RHW33_002210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV1645708.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSNEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            11507..11905
                     /locus_tag="RHW33_002211"
     CDS             11507..11905
                     /locus_tag="RHW33_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV1645709.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQPVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12020..12832
                     /gene="yidA"
                     /locus_tag="RHW33_002212"
     CDS             12020..12832
                     /gene="yidA"
                     /locus_tag="RHW33_002212"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV1645710.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12878..13534)
                     /locus_tag="RHW33_002213"
     CDS             complement(12878..13534)
                     /locus_tag="RHW33_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645711.1"
                     /translation="MKLNFKGFFKAASLFPLALMLSGCISYALVSHTAKGTSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(13780..14844)
                     /gene="cbrA"
                     /locus_tag="RHW33_002214"
     CDS             complement(13780..14844)
                     /gene="cbrA"
                     /locus_tag="RHW33_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV1645712.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLTVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFATLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            14945..16159
                     /locus_tag="RHW33_002215"
     CDS             14945..16159
                     /locus_tag="RHW33_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV1645713.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSDKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPDGERVSFTYNDHVMHQLDSALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPSSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKATGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(16161..16493)
                     /locus_tag="RHW33_002216"
     CDS             complement(16161..16493)
                     /locus_tag="RHW33_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV1645714.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    16733..16805
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            16799..17212
                     /gene="ibpA"
                     /locus_tag="RHW33_002217"
     CDS             16799..17212
                     /gene="ibpA"
                     /locus_tag="RHW33_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV1645715.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            17324..17752
                     /gene="ibpB"
                     /locus_tag="RHW33_002218"
     CDS             17324..17752
                     /gene="ibpB"
                     /locus_tag="RHW33_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV1645716.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            17948..19609
                     /locus_tag="RHW33_002219"
     CDS             17948..19609
                     /locus_tag="RHW33_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV1645717.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIRTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(19606..20322)
                     /locus_tag="RHW33_002220"
     CDS             complement(19606..20322)
                     /locus_tag="RHW33_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1645718.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            20618..22234
                     /locus_tag="RHW33_002221"
     CDS             20618..22234
                     /locus_tag="RHW33_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV1645719.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22234..23556
                     /locus_tag="RHW33_002222"
     CDS             22234..23556
                     /locus_tag="RHW33_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDV1645720.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVHYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPQQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGKSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(23553..24446)
                     /locus_tag="RHW33_002223"
     CDS             complement(23553..24446)
                     /locus_tag="RHW33_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV1645721.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            24613..26328
                     /locus_tag="RHW33_002224"
     CDS             24613..26328
                     /locus_tag="RHW33_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV1645722.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            26325..27818
                     /locus_tag="RHW33_002225"
     CDS             26325..27818
                     /locus_tag="RHW33_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV1645723.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPSEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARTEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYELGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVNTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDARPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(27865..28314)
                     /locus_tag="RHW33_002226"
     CDS             complement(27865..28314)
                     /locus_tag="RHW33_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645724.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVERKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            28423..28770
                     /locus_tag="RHW33_002227"
     CDS             28423..28770
                     /locus_tag="RHW33_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV1645725.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            28760..29125
                     /locus_tag="RHW33_002228"
     CDS             28760..29125
                     /locus_tag="RHW33_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV1645726.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIEKRVA"
     gene            29122..29619
                     /locus_tag="RHW33_002229"
     CDS             29122..29619
                     /locus_tag="RHW33_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV1645727.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDTLQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDSTLQKWCGSENP
                     QSRRDCQACDWHRLCPHAREETPDSMLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(29627..30811)
                     /gene="emrD"
                     /locus_tag="RHW33_002230"
     CDS             complement(29627..30811)
                     /gene="emrD"
                     /locus_tag="RHW33_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV1645728.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNGGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(31091..31180)
                     /gene="tisB"
                     /locus_tag="RHW33_002231"
     CDS             complement(31091..31180)
                     /gene="tisB"
                     /locus_tag="RHW33_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV1645729.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            31745..31843
                     /gene="ivbL"
                     /locus_tag="RHW33_002232"
     CDS             31745..31843
                     /gene="ivbL"
                     /locus_tag="RHW33_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV1645730.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            31949..33637
                     /gene="ilvB"
                     /locus_tag="RHW33_002233"
     CDS             31949..33637
                     /gene="ilvB"
                     /locus_tag="RHW33_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV1645731.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPVRVRELAEKVQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRRGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            33641..33931
                     /gene="ilvN"
                     /locus_tag="RHW33_002234"
     CDS             33641..33931
                     /gene="ilvN"
                     /locus_tag="RHW33_002234"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV1645732.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            34193..>34803
                     /locus_tag="RHW33_002235"
     CDS             34193..>34803
                     /locus_tag="RHW33_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001626294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645733.1"
                     /translation="MASPINSGIMMSNLCSSTFLDKNINIAAELDITNNEKKYSPGSN
                     FAKWMLQEIKRLIANIMSGSRSVNTEILNYFHPMPGTENNGNRTWMAATGENEYTVIE
                     QTGGKSFNVFQVGAGKNNIKEILGNTSYSGVAVATIIKSLSEKTAALETHSADTVLRK
                     KLVNSIVMMNTDCSYEMPADIVSNTYDLLNLRIQKDEGHLPVQ"
BASE COUNT         8434 a         8776 c         9180 g         8413 t
ORIGIN      
        1 tgtttaaaaa tgaattaaat aaagaagata tattgaaact gatggcagca agagaaaaag
       61 tggcgggagc atcagacaaa tggacgaagg catcaggctt atattctgcg atagtgaaag
      121 ggcatacgga gattgttgct gcctggatgg agacagctga agtgatagcc agccattatg
      181 aaaatgataa agatgtagtg agagaactac tgtcgttaag cagaaataat gctgcctgct
      241 ctttgcatat tgcaagcttt aagaaaatga gtaaagaggt cattgacgta tatctgaatg
      301 cggcgattca cctggcgttg caacacgggt tcacttttga tgaaattttg gatcagttta
      361 cccgtgactt tgatgggaag tcattctctc atgtgataac taacgaggat gatatacata
      421 tgggactttg gctgaaaata ttcaaaattg tggttggtga aaatgaaaat taccttaaag
      481 atgtgatgat gcaactggaa gcgaagaata atgaaggtaa atctgtaata agtcaggcaa
      541 atggcaatcc tgtatttaaa gaactattct ggaaagcaat agacgaattt aactttccgc
      601 aagaggagct taatcggtta aaccaatatc gttctctgta aagacaggac tgcaagatgt
      661 tgaaagtcgc tccagccagt agtaaaggca gttttggtgc aggatgctca taaaaaaaca
      721 agcgctgttc cgttctgata gtagaacgga acagtgccct ttatatcaat taagacgact
      781 tacttaccaa tacagaagct ggagaaaatc cgccccagca ggtcgtcaga ggtaaattcc
      841 ccggtgattt cgcttaagtt ctgctgcgcc aggcgcaact cttccgccag tagttcgcct
      901 gcccaggcac ctaacagttg cgctttacct tgctgcaaat gctccgctgc ctgttccagc
      961 gcctgtaggt ggcgacggcg cgccaggaag ccgccttcca tgttggtgtc aaagcccatg
     1021 ctctgtttga gatggttacg cagcacgtcc acgccttcgc cagtccttgc agacagacga
     1081 attaacgcgt gaccgttcac ttcacttatt cccagcgttt cgccggtgat atcggcttta
     1141 ttgcgcacca cggtgatcgg cagtttcgct ggcagacggg cgataaattc cggccagatc
     1201 tctgccggat caacggcgtc tgtcgtggtg ccatcgacca taaacagcac gcggtcggcc
     1261 tgttcaattt cctgccacgc gcgctcgatc ccaatacgtt caacttcgtc actggcttca
     1321 cgtagcccgg cggtatcgat gatatgcagc ggcattccgt caatatggat atgctcacgt
     1381 agcacgtcac gcgtggttcc ggcgatatcg gttacgattg ccgcttcacg gcccgccagc
     1441 gcgtttaaca ggctcgattt accggcatta ggacgcccgg caatcactac tttcatccct
     1501 tcgcgcaaca aactaccctg acgtgcttca gcacgcactg catcgagatc ggcaatcacg
     1561 tcattgagct gggcttcaat tttgccgtcg gagaggaaat cgatctcttc atcagggaaa
     1621 tcaattgccg cttcgacgta aatgcgcaag tgggtgaggg cttccaccag atggttgacc
     1681 cgtgcggaga atgcgccttg cagcgagtta agtgccgaac gggctgcctg ttccgaactg
     1741 gcatcgataa gatcggcaat cgcctcggcc tgggctaagt cgagtttatc gttaagaaac
     1801 gcgcgttcgg aaaactcacc agggcgagca atccgcaggc cgggaatggt cagaatgcgt
     1861 tttaacagca ggtcgaggat caccggaccg ccatgacctt gcagttccag cacatcttcg
     1921 ccggtgaacg agttcgggcc agggaaccat agcgcaatcc cctgatcgag cacgctgccg
     1981 tcggcgtctt taaacggaag ataatcggcg tagcgcggct taggcagttt acccagcacg
     2041 gtttcggcaa cttcacgggc tttgaagccg gagatgcgca ggatgccaac gccgccacgt
     2101 cccggaggcg tggcctgggc tacgatagtg tcattatcgc tcatgatgtt cctgttgctt
     2161 tgtgtggcgg atgcgcggtg cttatccgcc ctacgaaaag aaaaaaggcg gtcaactgac
     2221 cgcccttatt ttagcgaaaa ctcaccgaat caggattttt tcttctcgcg gctatgcagg
     2281 ccacgttttt ccagaccacg gtaaatcagc tgctgctgaa taatggttac caggttgctg
     2341 acgatatagt acagcaccag acctgacggg aaccacagga agaacacggt gaagatgacc
     2401 ggcataaagg tcatgatctt ctgctgcatc gggtcggtca cagtggtcgg cgacatcttc
     2461 tgaatgaaga acatcgttac gcccatcagg atcggcagga tgtagtacgg gtcctgtgca
     2521 gacaggtcgt ggatccacag tgcaaacggt gcctgacgca gttcaacgga acccatcagc
     2581 atgtagtaca acgccaggaa gattggcatc tggatcagca gcgggaagca gccgcccagc
     2641 gggttaacct tctcagcttt gtacagcgcc atcatttcct ggctgatacg ctgtttgtca
     2701 tcgcccagac gctcacgcat tgcctgaatc ttcggctgca gcatacgcat cttcgccatg
     2761 gaggtgtact gagctttggt cagcgggtac atgatgccac gaacgataaa ggtgatgatg
     2821 ataatggaga agccccagtt acccacaaag ctatggatcc atttcagcag tttgaacagc
     2881 ggctgagaga tgaaccacaa ccaaccgtaa tcaacggtca gatccaggtg cggagcaaca
     2941 gctgccattt tgtcctggat ttccgggcca acccacaggg tgctgttcat cgcgccagtc
     3001 tgaccaggct gaaccagtac cggctgagat ttatagccga tagcggcgat gccgttaccc
     3061 agattagcgg tatagaagtt attggtaccg tcgttatgcg ggatccacgc cgtcgcgaaa
     3121 tactgttgca gcattgccac ccaaccacct ttcgaagaga tgttcaggtt ttcgttatcg
     3181 gcaatggtat cgaacttgta tttctcgtac ttctcgtcag gcgtagagta cgccgcgcca
     3241 cggaaggtgt gcagtgcgaa gttgctgctt ccggtatcga gatgcggtgg cagagtgatg
     3301 gattgcttca actgaccaaa cgaggagatt tccagcggtt tctcgtcagc gttctgcacg
     3361 ttgtagttga cgttgacagc gtaatcaccc cgtttcagga caaacgtttt ggtaaacgtg
     3421 ttgcctgccg cgtcggtata cgtcatcggc acctgcagtt cgttttgacc ttcagccagc
     3481 acataagcgt ctttttcaac gttatacagc ggacgcgggc cgttagccgg gttatccggg
     3541 ccatcacgac cggtcagacc gctctgtgcc tgataaataa actgcggtga agtttccagc
     3601 agctggaacg gctgggtaga gttcagctct ttcgggtaag caggcagcag agcttgctca
     3661 acatcaccac cacgggtgtt gatggtcaga tcaagcacgt cggtcttaac cgagatcagt
     3721 ttcccctggc cactggccgg tacgccctgg tcggcggcgc tacccgctgc ggtggtcgtt
     3781 gtctgcgtgg tctgttgggc ctgaggttgc gggtttttat cctgctccca ggcttgccag
     3841 atcatgaaag acacgaacag caaagcgatg actaaaagat tgcgttgcga atccatcgtt
     3901 agtgttctct ggtatcaaat ggtccgggcg ggacgggatc gtcaccacca gggtgtaaag
     3961 ggtggcattt taatacgcgt ttcaccgtca accaactgcc ttttatcact ccaaacctgc
     4021 gcaatgcctc aattccgtag cttgaacagg ttggagtgaa acgacaatgc ggcccgagta
     4081 gcggactaat caggcgttga tagacccgaa tgagggctat caggacccgc gagccaggcg
     4141 acagtggtgg cgccataatt tttccaacgc ttccgagaga gcacggttat cgaggtcggc
     4201 aacccctttt ttcgccacca ccacgaaatc catagccggg agttcatgtt ggcgcagacg
     4261 gaagctttca cgcgtcagac gtttaatccg attgcgttca tgggcgcgtc gaacgttttt
     4321 cttggcgact gtaagaccga tacggggatg ccccagcgaa ttcaggcggc cgagaatggt
     4381 aatttgcggc gtgccagccc gttgtggctg ctggaagacg aatgtgaatt gactgggagt
     4441 taacaagcgt aactccctgg gaaatgcgag cttaaccact caggggttag ctttattact
     4501 tagaaacggt cagacgagcg cggcctttag cacgacgacg tgccagaacc tgacgaccat
     4561 ttttagtagc catacgagca cggaagccgt gagaacggtt gcgcttcagt acagacggtt
     4621 gaaaagtgcg tttcatggcg atttctacct aaacttgaat aaattcaatg gctttattgg
     4681 atatccgccg aaaaatgaaa cgatggacac cgaagccatg ggtgattaaa gaggccggat
     4741 tgtaataatt gtacactccg gagtcaattc tctttcctta tttaccgcgc ttttccgcac
     4801 cttttcgcag ggaaaacgta cggcctcaca ccagtgaaaa ccagcatggc gcgccgggtg
     4861 gaggattata cgggctgacg ggtaaagcgc aaggatcgtc ctggatcttt attagatcga
     4921 ttaagccaat ttttgtctat ggtcattaaa ttttccaata tgcggcgtaa atcgtgcccg
     4981 cctcgcggca ggatcgttta cacttagcga gttctggatc atcctgtgga taaatcggga
     5041 aaatctgtga gaaacagaag atctcttgcg cagtttaggc tatgatccgc ggtcccgatc
     5101 gttttgcagg atcttgatcg ggcatataac cgcagacagc ggttcgtgcg tcaccctcaa
     5161 gcagggtctt ttcgacgtac gtcaacaatc atgaatgttt cagccttagt cattatcgac
     5221 ttttgttcga gtggagtccg ccgtgtcact ttcgctttgg cagcagtgtc ttgcccgatt
     5281 gcaggatgag ttaccagcca cagaattcag tatgtggata cgcccattgc aggcggaact
     5341 gagcgataac acgctggccc tgtacgcgcc aaaccgtttt gtcctcgatt gggtacggga
     5401 caagtacctt aataatatca atggactgct aaccagtttc tgcggagcgg atgccccaca
     5461 gctgcgtttt gaagtcggca ccaaaccggt gacgcaaacg ccacaagcgg cagtgacgag
     5521 caacgtcgcg gcccctgcac aggtggcgca aacgcagccg caacgtgctg cgccttctac
     5581 gcgctcaggt tgggataacg tcccggcccc ggcagaaccg acctatcgtt ctaacgtaaa
     5641 cgtcaaacac acgtttgata acttcgttga aggtaaatct aaccaactgg cgcgcgcggc
     5701 ggctcgccag gtggcggata accctggcgg tgcctataac ccgttgttcc tttatggcgg
     5761 cacgggtctg ggtaaaactc acctgctgca tgcggtgggt aacggcatta tggcgcgcaa
     5821 gccgaatgcc aaagtggttt atatgcactc cgagcgcttt gttcaggaca tggttaaagc
     5881 cctgcaaaac aacgcgattg aagagtttaa acgctactac cgttccgtag atgcactgct
     5941 gatcgacgat attcagtttt ttgctaataa agaacgatct caggaagagt ttttccacac
     6001 cttcaacgcc ctgctggaag gtaatcaaca gatcattctc acctcggatc gctatccgaa
     6061 agagatcaac ggcgttgagg atcgtttgaa atcccgcttc ggctgggggc tgactgtggc
     6121 gatcgaaccg ccagagttag aaacccgcgt ggcgatcctg atgaaaaagg ccgacgaaaa
     6181 tgacattcgt ttgccaggcg aagtggcgtt ctttatcgcc aagcgtctac gatctaacgt
     6241 acgtgagctg gaaggcgcgc tgaaccgcgt tattgctaac gccaacttta ccggacgggc
     6301 gatcactatc gacttcgtgc gtgaggcgct gcgcgacttg ctggcattgc aggaaaaact
     6361 ggtcaccatc gacaatattc agaagacggt ggcggagtac tacaagatca aagtcgcgga
     6421 tctcctttcc aagcgtcgat cccgctcggt ggctcgtccg cgtcagatgg cgatggcgct
     6481 ggcgaaagag ctgactaacc acagtctgcc agagattggc gatgcgtttg gtggtcgtga
     6541 ccacactacg gtgcttcatg cctgccggaa gatcgagcag ctgcgtgaag agagccacga
     6601 tatcaaagaa gatttttcaa atttaatcag aacattgtca tcgtaaacct atgaaattta
     6661 ccgtagaacg tgagcattta ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc
     6721 gtcctacgct accgattctc ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc
     6781 tgaccggtac tgatctcgag atggaaatgg tggcacgcgt tgcgctggtt cagccacacg
     6841 agccaggagc gacgaccgtt ccggcgcgca aattctttga tatctgccgt ggtctgcctg
     6901 aaggcgcgga aattgccgtg cagctggaag gtgaacggat gctggtgcgc tccgggcgta
     6961 gccgtttttc gctgtctacc ctgccagcgg cggatttccc gaacctcgat gactggcaga
     7021 gtgaagtcga atttaccctg ccgcaggcga cgatgaagcg tctgattgaa gcgacccagt
     7081 tttctatggc gcatcaggac gttcgctatt acttaaacgg tatgctgttt gaaaccgaag
     7141 gtgaagaact gcgcaccgtg gcgaccgacg gccaccgtct ggcggtctgt tcaatgccaa
     7201 ttggtcaatc tttgccaagc cattcggtga tcgtgccgcg taaaggcgtg attgaactga
     7261 tgcgtatgct cgacggcggc gataatccgc tgcgcgtgca gattggcagc aacaatattc
     7321 gcgcccacgt tggcgacttt atcttcacct ctaaactggt ggatggtcgc ttcccggatt
     7381 accgccgcgt tctgccgaag aatccggaca aacatctgga agcaggctgc gatctcctca
     7441 agcaggcgtt tgctcgcgcg gcaattctct ctaacgagaa attccgcggt gtgcgcctgt
     7501 atgtcagcga aaaccagctg aaaatcaccg ccaacaaccc ggaacaggaa gaagcggaag
     7561 agatcctcga cgttacctat agcggtgcgg agatggaaat cggcttcaac gtcagctatg
     7621 tgctggatgt cctgaacgcg ctgaaatgtg aaaacgtccg catgatgctg accgattcgg
     7681 tttccagcgt gcagattgaa gatgcagcca gccagagcgc ggcttatgtt gtcatgccaa
     7741 tgagactgta atgtccctca cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc
     7801 ggatctcgct ttatctcccg gctttaactt tctggtaggt gccaacggca gtggcaaaac
     7861 cagcgtgctg gaagccatct atacgctcgg ccatggtcgg gcgtttcgca gtttgcagat
     7921 tggtcgcgtc attcgccacg agcaggaggc gtttgttctc cacgggcgtt tgcagggcga
     7981 agagcgcgag acggcgattg gtttaaccaa agacaaacag ggcgacagca aagtccgtat
     8041 tgacggtacg gacgggcata aagtcgcgga actggcgcac ctgatgccga tgcagctgat
     8101 aacgccagaa gggtttactt tactcaacgg cggccccaaa tacagaagag cattcctcga
     8161 ctggggatgc tttcacaacg aacccggatt tttcactgcc tggagcaatc tcaagcgatt
     8221 gctcaagcag cgcaatgcgg cgctgcgcca ggtgacacgt tacgaacagc tacgcccgtg
     8281 ggataaagaa ctgatcccgc tggcggagca aatcagcacc tggcgcgcgg agtatagcgc
     8341 cggtatcgcg gccgatatgg ctgatacctg taagcaattt ctccctgagt tttctctgac
     8401 tttctctttc cagcgcggct gggagaaaga gacagaatat gctgaggtgc tggaacgtaa
     8461 ttttgaacgc gatcgccagc taacctacac cgcgcatggc ccgcataaag cggacttacg
     8521 cattcgcgcc gacggtgcgc cggtggaaga taccttatcg cgtgggcagc ttaagctgtt
     8581 gatgtgcgcc ttacgtctgg cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg
     8641 tctctacctg atagatgatt ttgcctctga gcttgatgat gagcgtcgcg ggctgcttgc
     8701 cagccgctta aaagcgacgc aatcgcaggt ctttgtcagc gcgatcagtg ctgaacacgt
     8761 tatagacatg tcggacgaaa attcgaagat gtttaccgtg gaaaagggta aaataacgga
     8821 ttaacccaag tataaatgag cgagaaacgt tgatgtcgaa ttcttatgac tcctccagta
     8881 tcaaagtcct gaaagggctg gatgcggtgc gtaagcgccc gggtatgtat atcggcgaca
     8941 cggatgacgg caccggtctg caccacatgg tattcgaggt ggtagataac gctatcgacg
     9001 aagcgctcgc gggtcactgt aaagaaatta tcgtcaccat tcacgccgat aactctgtct
     9061 ctgtacagga tgacgggcgc ggcattccga ccggtattca cccggaagag ggcgtatcgg
     9121 cggcggaagt gatcatgacc gttctgcacg caggcggtaa atttgacgat aactcctata
     9181 aagtgtccgg cggtctgcac ggcgttggtg tttcggtagt aaacgccctg tcgcaaaaac
     9241 tggagctggt tatccagcgc gagggtaaaa ttcaccgtca gatctacgaa cacggtgtac
     9301 cgcaggctcc gctggcggtt accggcgaga ctgaaaaaac cggcaccatg gtgcgtttct
     9361 ggcccagcct cgaaaccttc accaatgtga ccgagttcga atatgaaatt ctggcgaaac
     9421 gtctgcgtga gttgtcgttc ctcaactccg gcgtttccat tcgtctgcgc gacaagcgtg
     9481 acggcaaaga agaccacttc cactatgaag gcggcatcaa ggcgttcgtt gaatatctga
     9541 acaagaacaa aacgccgatc catccgaata tcttctactt ctccaccgaa aaagacggta
     9601 tcggcgtcga agtggcgttg cagtggaacg atggcttcca ggaaaacatc tactgcttta
     9661 ccaacaacat tccgcagcgt gacggcggta ctcacctggc aggcttccgt gcggcgatga
     9721 cccgtaccct gaacgcctac atggacaaag aaggctacag caaaaaagcc aaagtcagcg
     9781 ccaccggtga cgatgcgcgt gaaggcctga ttgcggtcgt ttccgtaaaa gtgccggacc
     9841 cgaaattctc ctcccagacc aaagacaaac tggtttcttc tgaggtgaaa tcggcggttg
     9901 aacagcagat gaacgaactg ttggcggaat acctgctgga aaacccaact gacgcgaaaa
     9961 tcgtggttgg caaaattatc gatgctgccc gtgcccgtga agcggcgcgt cgcgcgcgtg
    10021 aaatgacccg ccgtaaaggt gcgctcgact tagcgggcct gccgggcaaa ctggcagact
    10081 gccaggaacg cgatccggcg ctttccgaac tgtaccttgt ggaaggggac tccgcgggcg
    10141 gctctgcgaa gcaggggcgt aaccgcaaga accaggcgat tctgccgctg aagggtaaaa
    10201 tcctcaacgt cgagaaagcg cgcttcgata agatgctctc ttctcaggaa gtggcgacgc
    10261 ttatcaccgc gcttggctgt ggtatcggtc gtgacgagta caacccggac aaactgcgtt
    10321 atcacagcat catcatcatg accgatgcgg acgtcgacgg ctcgcacatt cgtacgctgc
    10381 tgttgacctt cttctatcgt cagatgccgg aaatcgttga acgcggtcac gtctacatcg
    10441 ctcagccgcc gctgtacaaa gtgaagaaag gcaagcagga acagtacatt aaagacgacg
    10501 aagcgatgga tcagtaccag atctctatcg cgctggacgg cgcaacgctg cacaccaacg
    10561 ccagtgcacc ggcattggct ggcgaagcgt tagagaaact ggtatctgag tacaacgcga
    10621 cgcagaaaat gatcaatcgt atggagcgtc gttatccgaa agcaatgctg aaagagctta
    10681 tctatcagcc gacgttgacg gaagccgacc tttccaacga gcagaccgtt acccgttggg
    10741 tgaacgcgct ggtcagcgaa ctgaacgaca aagaacagca cggcagccag tggaagtttg
    10801 atgtccacac caatgccgaa caaaacctgt tcgagccgat tgttcgcgtg cgtacccacg
    10861 gagtggatac tgactatccg ctggatcacg aatttatcac cggcggcgaa tatcgtcgta
    10921 tctgcacgct gggtgagaaa ctgcgtggct tgctggaaga agatgcgttt atcgaacgtg
    10981 gcgagcgtcg tcagccggta gccagcttcg agcaggcgct ggactggctg gtgaaagagt
    11041 ctcgtcgcgg tctctccatc cagcgttata aaggtcttgg cgagatgaac ccggaacagc
    11101 tgtgggaaac caccatggat ccggaaagcc gtcgtatgct gcgtgttacc gttaaggatg
    11161 cgattgctgc cgaccagctg ttcaccacgc tgatgggcga cgccgttgaa ccgcgccgtg
    11221 cgtttattga agagaacgcc ctgaaagcgg cgaatatcga tatttaatgg cgttaaccat
    11281 gcgagcgtgc ctgatgcgct acgcttatca ggcctacgag actcctgcaa tgtattgaat
    11341 tttcatgatt ttgtaggccg ggtaagacgt taacgtcgca tccggcaaga agctataaga
    11401 aaagggcgga gatcatctcc gcccttttta tttctgcaat ccgcaattat catcccgcag
    11461 ttctatactt tttgggtcaa cacgtaacgc tttacaggtg attatcatgg ggctttttga
    11521 tgaagttgtc ggcgcctttc tgaaaggcga tgcggggaaa tatcaggcga ttttaagttg
    11581 ggttgaggag cagggcggca ttcaggtgct gctggaaaaa ctgcaaagtg gcggcttagg
    11641 tgccattctc tcaacctggt tgagtaatca acagagcaac caaccagtta gcggcgagca
    11701 actggaatca gcactcggca caaatgcggt gtccgatctc gggcaaaaac ttggcgtgga
    11761 taccagtaca gcttccagtt tactggcaga acaattgccg aagattattg atgcactctc
    11821 accgcaaggt gaagtgtcac cacaagccaa taacgatctg ctttccgcag gcatggaact
    11881 gctgaaaggg aaactcttcc gctaagcaaa tggggagcat gcaacccgcg ttgcatcccc
    11941 cttttccctc gggtgttgta ctgatttttg agcggaatcg cgttagcatg ggtcaggaac
    12001 caatctacct ggggaactca tggctatcaa actcattgct atcgatatgg atggcaccct
    12061 tctgctgccc gatcacacca tttcacctgc cgttaaaaat gcgattgctg cagctcgcgc
    12121 ccgtggcgtg aatgtcgtgc taacgacggg tcgcccttat gcaggtgttc acaattacct
    12181 gaaagagctg catatggaac agccgggcga ctactgcatt acttataacg gcgcgctggt
    12241 acagaaggcc gctgatggta gcactgtggc gcaaaccgct ctcagctatg acgactaccg
    12301 tttcctggaa aaactctctc gcgaagtcgg ttctcatttc cacgcactgg accgcaccac
    12361 gctgtacacc gccaaccgtg atatcagcta ctacacggtg catgaatcct tcgttgccac
    12421 tattccgctg gtgttctgcg aagcggagaa aatggacccc aatacccagt tcctgaaagt
    12481 gatgatgatt gatgaacccg ccatcctcga ccaggctatc gcgcgtattc cgcaggaagt
    12541 gaaagagaaa tataccgtgc tgaaaagtgc gccgtacttc ctcgaaatcc tcgataaacg
    12601 cgttaacaaa ggtacggggg tgaaatcact ggccgacgtg ttaggtatta aaccggaaga
    12661 aatcatggcg attggcgatc aggaaaacga cattgcaatg attgaatatg caggcgtcgg
    12721 tgtggcaatg gataatgcta ttccttcggt gaaagaagtg gcgaactttg tcaccaaatc
    12781 caaccttgaa gatggcgtgg cgtttgccat tgaaaagtat gtgctgaatt aatctgtggg
    12841 cgggcaaaca gcataaacgc gtttgcccgc ttactgatta ttggtttact gcatatacaa
    12901 ccagaaattg cagcggtgca gtaatgatgt ccagcgtaac agtcactggc agtaatgctg
    12961 cggaaacacc atccggaatg cctctgggtg aatagtattc ataaaatcgc actttaaatg
    13021 gatggtagaa cgccatcacc tttgagtaat ccatgttttt atttttctca tggattgtgc
    13081 cttttaggtt ttccagtgac ctggtacagt gttgaacacc acaggcaaaa ccataatgcg
    13141 ttgccagtgc cttttcttct tcgttattcc agtagtagta aagcgatatt gtgccggtaa
    13201 atttcttttt ccccgcattt aaaacaaatc ttgcgtcatc agcaacctga atattgtgcc
    13261 ggttaagtgc tggatcattg agcatcttaa cgatgtcatc agcaccatct gtgataaggt
    13321 aatccagcga ttcccctaca aaaacatagc cttttgttcc attactatcc tttgcctgcg
    13381 atagccccgt gatggtgtct gactgcgatt gatactttcc tgaagtaccc tttgcggtat
    13441 gggaaaccag agcatacgag atacagcctg aaagcatcag cgccagtggg aataaactgg
    13501 cagccttaaa aaatccctta aaattcaact tcatgattaa tcctttaaac gagtagctaa
    13561 gccgttgaaa gcatactctt ctttccctcc tgtttagcgc ctgatctccc ttttcagatt
    13621 tctgcccgat ataagacctt ttcttatgag gtgttttttt gtggactaat atctgttgca
    13681 aggctatggg gtcgccccgg cactggttta tatctccgcc gtcgcgttta ttggcgcgct
    13741 ctcttatatc ctgctggtgg gcgatgtgaa gcgcgttggc taatccttca actgtggaat
    13801 atgtgccaca ccactgcgca taatccactt tcttaaagcc ggtgcggtca ggcatcggct
    13861 ttttactatc ttgccgaaga gttttaaacg cagtttgcgg gtggcgcgcc agtaagcggt
    13921 attgagcttc tctggctgct tcagtaacac cgaacgcaga atgtctgcgc tatccagcgc
    13981 atagctaatc ccctccagcg agctggcgct gataaatccc gccgcttcac caatcagaaa
    14041 ggcgttgtcc ttaccgcaga caaaatcctg ccagcgtgag ggaaacagca ccgtgcattt
    14101 ttcgcttttc accgccttac caaactggaa ctgaaaggcg ctcattttct ctttcagcgt
    14161 cgcgaaacgc gtctgaccgt ctttcattgg ataggcaccg ccaaagataa aataaccgtc
    14221 tttgctgata ctccatgagt aacagtcagt tatcgcatta tcaaagatgc aggagtagaa
    14281 cggcaccgga tgtttctctg cgaaccactg ctggattgcg acatatttac ggatttgatg
    14341 atccgggtag agatgtcgcc gcaccatcga gttggcacca tcggcaccga ccagatagcg
    14401 ggcggtaata tgctgctccc aaccgtctgc acgaaaaatg acatgccatt tatcatcctc
    14461 acgccagatt tttcggcaca ggctatcgtg ataaacctca acgctggcgg ggatcagcga
    14521 tttcatccac aagtcgaaag catggcgatt aatattgata tagcttcgct ggtaattacg
    14581 cgtgagcgat gcggcgacgt cgacggtttt gacgctgaaa atctgcggat tagcgatcac
    14641 atcgacagta agcgtcagcc catcgcgaat aaaagatcgc tgcgcgtccg gtgccagcag
    14701 accgccacaa ggtttgctga aaccttcagt accatgctgg tgctttttat ccagcgcgat
    14761 cacctgcatt ttgccggcta actttcgtgc caacgccgac cctgccgggc cgaggccaat
    14821 aatcgccacg tcgaaatgtt ccatcttttc actcacattc atcacgatgg caaaagacta
    14881 ttgtctgaaa gtgaagtcct tgtgaagtat gatggctgga cccgttttgt atcaggaccg
    14941 cgccatgaaa cagataacct ttgctccccg taatcacctg ctcaccaata ccaatacctg
    15001 gacgcccgac agccagtggc tggtatttga cgtgcgtcct tctggggcgt cgtttaccgg
    15061 cgagaccatt gagcgtgtga atatccatac cggcgaggtc gaggttatct atcgcgcgtc
    15121 acagggcgca cacgtcggcg tggtgaccgt tcatccgaag tcagataaat atgtctttat
    15181 tcacggccct gaaaatcctg atgaaacatg gcattacgat ttccatcatc gacgcggagt
    15241 cattgctgaa ggcggcaagg tgagcaatct cgatgcaatg gatattactg caccgtacac
    15301 cccaggagcg ctgcgcggcg gcagccatgt acatgtcttt agcccggacg gtgaaagggt
    15361 gagctttacc tataacgacc atgtaatgca tcaactcgat tcggcgctgg atttgcgaaa
    15421 cgtcggtgtt gcggcaccgt ttggcccggt caacgtacaa aagcagcatc cgcgcgaata
    15481 cagcggtagc cactggtgcg tgctggtgag taaaaccacg cccacgccgc agcctagcag
    15541 cgatgaaatc aatcgtgctt atgaagaagg atgggtagga aatcacgcgc tggcgtttat
    15601 tggcgacaca ctttcgccaa agggcgagaa agtgccggag ctatttatcg ttgagttacc
    15661 gcaagatgaa gctggctgga aagcgacagg tgatgcgccg ttaagtggaa cggaaacaac
    15721 cctgcccgcg ccaccgcgtg gcgtcgtgca gcgacgttta acctttaccc accatcgggc
    15781 ttatccgggg ttagtcaacg tcccgcgcca ctgggtgcgc tgtaatccgc agggtacgca
    15841 aatcgcgttt ttaatgcgtg atgataacgg cattgtgcaa ctgtggctta tctcgccaca
    15901 gggcggcgag ccgcgccagt taacccataa caaaacggat attcagtctg catttaactg
    15961 gcatccatca ggagaatggt tgggctttgt gctggataat cgaattgctt gtgcccatgc
    16021 gcaaagcggc gaggttgagt atttaaccga aaaccacgcc aatccgcctt ctgcggacgc
    16081 tgtggtcttc tcgccggatg gtcagtggct ggcgtggatg gaaggcggcc aactgtggat
    16141 caccgaaact gatcgctaaa tcagttatca ggcattctgg ccggtggaat aaccgcattg
    16201 gtggcttgcg cgttggcttc gcttttttca acgcgtgagc gcaccgagct gtctttgcgg
    16261 aacacatccc acggcagtaa aagcgtgtcc atcacagcgg taaacggcat atcgagaatc
    16321 gccagcgact tagtgcccca gttggtttca tcgtcgccaa tcatggtggc gctggcgcgg
    16381 gtgccgggat acgttccttc tttccccccg gtatgggaca tcacgctgga gcatcctccg
    16441 agtaatgtca ttccgctgca tatcatgaac gctaacaaca catttcttat cataataaat
    16501 tcatctgttg atcgtgggtg ttggcctgat gagttatagc gatcccttgc tgaaaataac
    16561 atcatcatta cgtcgctctg tggcggctat cgcactttaa cgtttcgtgc tgccccctca
    16621 gtctatgcaa tagaccataa actgcaaaaa aaagtccgct gataaggctt gaaaagttca
    16681 tttccagacc catttttaca tcgtagccga tgaggacgcg cctgatgggt gttctggcta
    16741 cctgacctgt ccattgtgga aggtcttaca ttctcgctga tttcaggagc tattgattat
    16801 gcgtaacttt gatttatccc cgctttaccg ttctgctatt ggatttgacc gtttgtttaa
    16861 ccacttagaa aacaaccaga gccagagtaa tggcggctac cctccgtata acgttgaact
    16921 ggtagacgaa aaccattacc gcattgctat cgctgtggct ggttttgctg agagcgaact
    16981 ggaaattacc gcccaggata atctgctggt ggtgaaaggt gctcacgccg acgaacaaaa
    17041 agagcgcacc tatctgtacc agggcatcgc tgaacgcaac tttgaacgca aattccagtt
    17101 agctgagaac attcatgttc gtggggctaa cctggtaaat ggtttgctgt atatcgatct
    17161 cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc gaaatcaact aattccctaa
    17221 ggccgcctgg cgcggcctga catctccatg ctcgccgtca gggagcatat gcgaatcttc
    17281 ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta acttcgattt
    17341 atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac tgcaaaacgc
    17401 cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa agcgacgata accactaccg
    17461 cattaccctt gcgctggcag gtttccgtca ggaagattta gagattcaac tggaaggtac
    17521 gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa gagaaaaaat ggctgcatca
    17581 agggcttatg aatcagccat ttagcctgag ctttacgctg gctgaaaata tggaagtctc
    17641 tggcgcaacc ttcgtaaacg gtttactgca tattgattta attcgtaatg agcctgaacc
    17701 catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg ttaaatagct aactagctat
    17761 tctctttgcc ccgccattct ggtggggctt tttttgtctt tcttcttcaa caatgcgtta
    17821 aatttcgact gtttaagata tttcggcacg ttctttcccg tgataatgtg cgctataagg
    17881 caaatctgct tcaccgattc agccataatc cgtagcaatt aaaatgttat ctcaagagaa
    17941 atggacgatg agtgatatag cattaacggt cagtattctg gctttggtgg cagtcgtcgg
    18001 tttgtttatc ggcaacgtca aatttcgcgg cataggatta ggtattggcg gcgtgctgtt
    18061 tggtgggatc atcgtcggcc attttgtttc tcaggcgggg atgacattaa gtagcgatat
    18121 gctgcatgtt attcaggaat ttggcctgat cctgttcgtt tatactatcg ggattcaggt
    18181 agggccgggc ttctttgcct cattgcgcgt ctccggatta cgcctcaacc tgtttgctgt
    18241 tctgatcgtc atcatcggtg gtctggttac cgccatcctg cataaactgt ttgatattcc
    18301 actgccggta gtgctgggga ttttctccgg tgcggttacc aatacgccag cgctgggggc
    18361 agggcagcag atcttgcgcg acctgggtac accaatggaa atggtcgatc agatggggat
    18421 gagttacgcg atggcgtatc cattcggcat ttgcgggatt ttgttcacca tgtggatgtt
    18481 gcgggttatt ttccgcgtca atgtcgagac agaagcccag cagcacgaat cttcacgcac
    18541 caatggcggc gcgctgatca ggactatcaa tattcgcgtt gagaacccta acctgcatga
    18601 tttagccatt aaagatgtac cgattctcaa cggcgacaaa attatctgct cgcgtctgaa
    18661 acgcgaagaa accctaaaag ttccttcgcc agataccatt atccaactgg gcgatttgct
    18721 gcatctggtg ggtcagccag cggatttaca taatgcgcaa ctggtgattg gtcaggaggt
    18781 cgatacttcg ctgtccacga aaggcactga tttgcgcgtc gagcgtgtgg tggttaccaa
    18841 tgaaaacgtg cttggtaagc gtattcgcga cctgcatttt aaagaacgct atgacgttgt
    18901 tatctcgcgc ctgaaccgtg ccggggtcga actggtcgcc agtggcgata tcagcctgca
    18961 gttcggcgat atcctcaatc tggtggggcg tccgtccgca attgatgccg ttgccaatgt
    19021 gctggggaat gcgcagcaaa aactgcaaca ggttcagatg ctgccagtgt ttattggcat
    19081 cgggctaggc gtattgttag gttctattcc cgtctttgtg ccgggattcc cggccgcgtt
    19141 gaaactgggg ctggcgggcg gtccgctgat tatggcgttg atcctcgggc gtatcggcag
    19201 tatcggcaag ctgtactggt ttatgccgcc aagtgccaac ctcgcgctgc gggagctggg
    19261 gatcgtgctg ttcctctcgg tcgttggtct gaaatctggt ggggattttg taaataccct
    19321 ggtcaatggc gaagggctaa gctggattgg ttatggtgcc ctgatcaccg ccgttccgct
    19381 gattactgtt ggcattctgg cgcggatgtt agccaaaatg aattacctga ccatgtgcgg
    19441 gatgctggca ggttccatga ccgatcctcc ggcgctggcg tttgctaata atcttcatcc
    19501 aaccagcggt gcggcggcgc tctcttacgc cactgtctat ccgttggtga tgttcctgcg
    19561 cattatcacc ccccaattac tggcggtgct cttctggagt atcggttaac tcttttccgg
    19621 gtggagtcgc cgtaaatggt actccacctg gtattcgctg gcatttctga acatcactga
    19681 ataattcaaa aactccccgc tctcgctata tgacaacgag gtgatccgca gcagtggcgt
    19741 gtgttccgct accttcattt gccgcgccag cagtctatcg gcgagcaccg gcgtcaggct
    19801 ttcataatta ccgccaatca gaatcccgca ctcttgttca atatattcaa acttcgaccc
    19861 ttccagatgt tgcagcgaaa ggttacggaa cagttttacc ggcatatagc tgtcttccag
    19921 catcagcggt ttcccttcca caaaacgaac gcgacgggag aagtagatct gctcgttgat
    19981 ttgaatccgt aactggctgg caatggccgg aggcgcaggc atgatttcaa aaatcagcac
    20041 ctggctggtg accgtttttc cctgacgttt taacacctcc accagcccgg tcaggctggc
    20101 ggtttgatgc agtacatctt tgcgtaccag gtaagtgccg ctgccgtggc ggcggaccac
    20161 cagcccccac gctaccagca ggtcaatcgc tttacggatg gtcatccgcg ataccgcaaa
    20221 ctcttctgcc agcttttttt caccgggaag aaggctgttt agcgtgaaat ctgcggaatt
    20281 aagtctaatt cttaaccgct ccgcaatgct tttgtagatc atctctagat ctcttttttg
    20341 cctgcaagcc cgggtaatgc gcccacaagt tctgcttaat cgattgaaaa aacatctttt
    20401 ttttaaagat gtgttcgatg ctgtgtacct gtgctcacag aggtctactt tttcgcgaaa
    20461 acgtagatct ctaccgccca acgaaaagca tgaaagcgat cacgaatccc attgggtcgt
    20521 catgttctgc cgatcgcatc ttcctatcct cgctccaggc ctgccgcata accaatcagg
    20581 cttcctactt acagaattga gaaaagagga tgtggaaatg ctcagtcaaa ttcaacgctt
    20641 tggcggcgcg atgttcacgc cagtgctgct gtttcccttc gccgggattg tggtgggtct
    20701 tgccatcttg ctgcaaaacc cgatgtttgt cggggaatca ctgaccgatc caaacagttt
    20761 attcgcgcaa atcgtacaca ttattgaaga gggcggttgg acggtattcc gtaatatgcc
    20821 gctgattttt gctgtcggtt tacccattgg ccttgctaag caagcgcagg ggcgtgcttg
    20881 tcttgcggtg atggtgagtt tcctgacctg gaactatttc atcaatgcga tgggaatgac
    20941 ctggggaagt tacttcggcg tcgatttcac tcaggacgcg gtggcaggta gcggtctgac
    21001 aatgatggcc gggattaaaa ccctcgatac cagcattatc ggcgcaatta tcatttccgg
    21061 cattgtgacg gcgctgcata accgtctgtt cgataaaaaa ctgccggtgt ttctcggcat
    21121 tttccagggg acgtcttatg tggtgatcat cgccttcctg gtgatgatcc cctgtgcctg
    21181 gctgacattg ctcggctggc caaaagtaca aatggggatt gaatctctgc aagcgttcct
    21241 gcgttcggcg ggtgcacttg gggtctgggt ttacaccttc ctcgaacgta ttctgatccc
    21301 aaccggttta caccacttca tctacggacc gtttatcttt ggtccggcag ctgttgaagg
    21361 cggcattcag atgtactggg cgcagcatct gcaagagttc agtttgagcg ccgagccgct
    21421 gaaatcgttg ttcccggaag gcggttttgc cctgcacggt aactcaaaaa tctttggtgc
    21481 cgtgggcatt tctttagcga tgtacttcac tgccgcaccg gaaaatcggg taaaagtggc
    21541 gggtttgctg attcccgcaa ccttaaccgc catgctggtg ggaattaccg aaccgctgga
    21601 atttaccttc ctgttcattt caccgttgct gtttgcggta cacgctgtgc ttgcggcctc
    21661 aatgtcgacc gtgatgtatc tctttggtgt ggtgggcaac atgggcggag gtctgattga
    21721 ccaggtttta ccgcaaaact ggatcccgat gttcagcaac cacgcggata tgatgctgac
    21781 ccaaatcgcc attgggttgt gctttaccct gctgtacttc gtggttttcc gcaccctgat
    21841 tctgcaattc aacatgtgca cgccgggacg tgaagatgcg gaagtgaaac tctactcaaa
    21901 agccgaatac aaagcctcgc gaggccaaac caccgcggca gagccaaaaa aagagctgga
    21961 tcaggctgcc ggtatcctgc aagccctggg cggggtcggc aatatctcca gcatcaacaa
    22021 ttgcgcgacg cgtctacgta ttgcactgca tgacatgtca caaacgctgg atgacgaagt
    22081 ctttaaaaag ctgggagcgc acggcgtctt ccgtagtggc gatgccattc aggtgatcat
    22141 tggtctgcat gtatcccagc tgcgtgaaca gctcgatagc ttaattaatt ctcatcaatc
    22201 agcagaaaat gttgccatta cggaggcagt ataatgacca aattctcagt ggttgtcgct
    22261 ggcggtggaa gcacctttac gccaggcatc gtgttgatgc tcctggcgaa tcaggaccgt
    22321 ttcccgcttc gtgcactgaa attttatgat aacgatggcg cgcggcagga agtgattgcc
    22381 gaagcctgta aagtcatcct taaagagaaa gcgccggaca ttgcgtttag ttacaccacc
    22441 gatcctgaag tggcgttcag cgacgttgat tttgtcatgg cgcacatccg cgtcggcaag
    22501 tacccgatgc gcgaactgga tgaaaaaatc ccgctgcgcc acggcgttgt tggtcaggaa
    22561 acttgcggac ccggcggaat agcgtacggc atgcgttcca ttggcggcgt cctggaactg
    22621 gtggattata tggaaaaata ttcaccaaac gcctggatgc tcaactactc caacccggca
    22681 gccattgtcg cagaagccac gcgtcgtctg cgcccgaatg cgaaaatcct caacatctgt
    22741 gacatgccaa tcggtattga aagccggatg gcgcaaattg ttgggctgca agatcgcaaa
    22801 cagatgcgcg tgcactacta cggcctgaac cactttggct ggtggacatc aattgaagat
    22861 ttgcagggca acgacctgat gccccagctg cggcaatatg tctcgaagca cggttatgtt
    22921 ccaccgcagc aagatacgca tactgaagcg agctggaacg acacctatgc aaaagcgcgg
    22981 gatgtccagg cactggaccc ggatacattg ccgaacacct atctgaaata ttatctcttc
    23041 ccggattatg tcgttcagca ttccaaccct gaacataccc gcgcgaatga ggtgatggaa
    23101 catcgcgaga aacaggtttt cgatgcttgc cgcgccatta cggcggcagg aaaatcagcg
    23161 gcgggcaagc tggaaattga cgaacatgcg tcatacatcg tcgatctggc ggcggcaatt
    23221 gccttcaaca ctcaggagcg gatgttgctg attgtgccta acaatggggc aattcataac
    23281 tttgatgatg aagcgatggt cgagatcccg tgtctggtcg ggcacaacgg accagaacca
    23341 ctggtggtcg gcgatatccc gcagtttcag aaagggttaa tgagtcagca agtggcggtg
    23401 gaaaaactgg tcgtggatgc ctgggaacag cgttcatatc agcacctgtg gcaggcgatt
    23461 acgttgtcga aaacggtacc gagcgcctcg gtcgccaaag ctattctgga tgagttgctg
    23521 gaggccaaca aagcgtactg gccagagtta cgttaattgt gtggactggc atccgttggg
    23581 tgctggtccg tattctttag cacggctgaa ggcgcataac caaatttatg tttaaaggct
    23641 ttgctgaaat gaaaggagtc gaagaagttg agcgtatcag cgacttcgcc aaccgagttt
    23701 ccttgttgct ttaatagcga tagcgccaga tccagacgcg catcgaggta atattctttc
    23761 ggcgtctttc cggtatagcg taaaaacaga cgacgcaacc aggcttcgct acaggggatc
    23821 gtggcagcca tatcggctac gctccagcgt tgttgcagac tggcatgtaa agtggcaatt
    23881 aatttttcaa tttgccgccg ttgtggatct tttttaccgt ctgcgtacat aagacaaatc
    23941 cactgataaa taattttcgt cagaaaagcg actgccagat tattttttat tgcttctggt
    24001 gaagttatta actcagcaac ttcggtgagt tcctgattat aaatttcgcc attataaata
    24061 acgctttgct gaccaacggg aatatccatc atactggtgg gggtaaattc catccagtac
    24121 tgttcccaga ctaaaccttc acagtgataa gagtgaatat ccattggctt taaaaatata
    24181 atacagttgc catgcagtgt tatttgttcg ccattcttta aaagaatttt cccacatcct
    24241 tgtacggtat acaccgcaac ccatgagttg gcgataagcg gtttcttctt gtcccgcggc
    24301 cagatcaccc ggtaatgctc gtcggcgtag gtatgccaga gcgagatcag agaaattccg
    24361 ctggagatca cattttcgtt aatcaataag ggaatattgt acggagtttc gttttttaca
    24421 tctgaacttt gcaattttcc attcatttta atatccctcc ggcgaattat gctcacaacc
    24481 tgaacttaac agaaaacaat ccgttcaggg atacgacgtt tgtattttaa gaatctgact
    24541 gcctgacccg acgcatttta cctctcccta tattcatgcg tccgggacat aaacataaat
    24601 aagggctatg agatgaattc gttacaaatc ttgagttttg tcggttttac gctgctggtg
    24661 gcgatcatca cctggtggaa ggttcgcaaa acagataccg gatcgcaaca aggctatttt
    24721 cttgccggac gttcactaaa agcgccggtt attgccgctt cgttaatgtt aaccaacctt
    24781 tccacggaac aactggttgg tctttccggg caggcctaca aaagcggcat gtcggtgatg
    24841 ggctgggaag tgacttcagc ggtgacgctg atcttcctcg cgctaatctt tttaccgcgc
    24901 tatctgaagc gcggcattgc caccatcccc gattttctgg aggaacgtta tgataaaacg
    24961 acgcgtatta tcatcgactt ctgcttcctc attgccaccg gcgtctgctt tctgccgatt
    25021 gttctctact ccggcgcgtt ggcgctcaac agcctgtttc acgtcgggga atcgctacag
    25081 atttctcacg gtgcggctat ctggctactg gtaattttgc ttggtctggc gggaattttg
    25141 tatgcggtga tcggcggact gcgcgcaatg gcagtggcgg actccatcaa cggtattggg
    25201 ctggttatcg gcgggttgat ggtgccggta tttggcctga tcgcgatggg caagggcagc
    25261 tttatgcagg gcattgagca aattaccacc gttcacgccg agaaattaaa ctcagtcggt
    25321 ggcccgaccg atcccttgcc gattggcgcg gcatttaccg gtttaattct ggtgaacacc
    25381 ttttactggt gtacaaatca gggcatcgtg caacgcacgc tggcgtcaaa aagcctggcg
    25441 gaagggcaaa agggggcgct gttaacggcg gtgctgaaaa tgctcgaccc gctggtactg
    25501 gtgctgccag ggttgattgc gtttcatctg tatcaggatc taccgaaagc cgacatggcc
    25561 tacccgacgc tggtcaataa cgttctgcca gtgccactgg tgggtttctt cggcgcggtg
    25621 ttatttggtg cggtgatcag taccttcaac ggctttctga atagcgccag tacgttattc
    25681 agtatgggta tttaccgtcg catcattaac cagaatgccg agccgcagca gctggtcacc
    25741 gtcgggcgca aatttggttt ctttatcgct atcgtttcgg tgctggtagc gccgtggatc
    25801 gccaacgcgc cgcaggggct gtatagctgg atgaaacagc tcaacggcat ttacaacgtg
    25861 ccgctggtta ccatcatcat tatgggcttt ttcttcccgc gcatcccggc gctggcggca
    25921 aaagtggcga tggggattgg cataatcagc tacatcacca tcaactatct ggtgaagttc
    25981 gacttccatt tcctctatgt gctggcctgt acgttctgca tcaacgtggt cgtgatgctg
    26041 gtgatcggtt ttatcaaacc gcgcgccacg ccgttcacct tcaaagatgc gtttgcggtg
    26101 gacatgaaac cgtggaaaaa cgtcaagatc gcgtcaattg gcatcctgtt cgcgatgatt
    26161 ggcgtctatg ccgggctggc tgaattcggc ggctacggta cgcgctggtt agcgatgatc
    26221 agttatttca ttgccgccgt agtgattgtc tacctgattt ttgacagctg gcggcatcgt
    26281 cacgacccag ccgtaacctt tactcccgac gcaaaggata gcctatgaaa cgccccaatt
    26341 ttctgttcat catgaccgat acccaggcca ccaatatggt cggttgctat agcggtaagc
    26401 cgctgaatac gcaaaatatt gatagtctgg cggcggaagg tattcgcttt aactccgcct
    26461 acacctgttc accggtttgt acaccggcgc gcgccgggct gtttaccggt atctacgcta
    26521 accagtccgg cccgtggacc aacaacgtcg cgccgggtaa aaacatctcc actatggggc
    26581 gctactttaa ggatgccggc tatcacacct gttacatcgg caaatggcat ctcgacggtc
    26641 atgactattt cggcactggc gagtgtccgt cggagtggga cgctgattac tggttcgatg
    26701 gggcgaacta tcttagcgaa ctgacggaaa aagagattag cctgtggcgc aatggcctaa
    26761 acagcgtcga agatttacag gcgaaccata ttgacgaaac cttcacctgg gcgcaccgca
    26821 tcagcaatcg agcggtggat tttctgcaac agcccgcgcg caccgaggaa cccttcctga
    26881 tggtggtttc gtatgatgag ccgcatcacc cgttcacctg tccggtggag tatttagaga
    26941 aatacgctga tttttactac gagctgggcg agaaagcaca ggatgacctg gcgaacaaac
    27001 cagaacatca ccgcttatgg gcgcaggcga tgccatcgcc agtcggtgat gacgggcttt
    27061 atcaccatcc gctctatttt gcctgtaatg actttgttga tgaccaaatc ggacgggtca
    27121 tcaatgcctt aacgccagag caacgtgaaa atacgtgggt tatttataca tccgatcacg
    27181 gcgaaatgat gggcgcacat aagctgatca gtaaaggagc ggcgatgtat gacgacatca
    27241 cccgtattcc gctgatcatc cgttcgccgc aaggggagcg gcgacaggtc aatacgccag
    27301 tcagtcatat cgatttactg ccgacaatga tggcgctggc agatattgaa aaaccagaga
    27361 ttctgccggg ggaaaatatc cttgccgtga aagagccacg cggcgtgatg gtggaattta
    27421 accgctacga gattgagcat gacagctttg gcggttttat tccggtgcgt tgctgggtga
    27481 cggatgactt taaactggtg ctcaacctct tcaccagtga tgaactttac gatcgccgta
    27541 atgatccaaa tgaaatgcat aacctgatcg atgatatccg ttttgccgac gtgcgcagca
    27601 aaatgcatga cgccttattg gattacatgg ataaaattcg cgatccgttc cgcagttacc
    27661 aatggagtct gcgtccgtgg cgtaaagatg cacgaccgcg ctggatgggg gcgtttcgtc
    27721 cacgcccaca agatggctat tcgccagtgg tacgcgacta tgacaccggc ctaccgacac
    27781 aaggggtgaa ggtggaagag aaaaaacaga agttctgacg ataatgcccg ctgatgatca
    27841 cccggcgggc attattcagg ctgcttaaac cagtgaaccg ccggtcagga aggaaaagat
    27901 aaggaatatc agcaaaatgc tgcacacgat gccgatacca aaaagcaagg tatggaaagc
    27961 cagcgtcccg ccgccaaaaa ccagcgcgcc gcgaatgccc cagcgatttt ccttacgcat
    28021 accgccaata aatgcgcaaa gtagcgcagc aatggccagt gcgataccgc tttgatcgag
    28081 gattttatcg acatccacgt cttcttttct ctccacggtg gtaatttttt cgccttttat
    28141 tccggcgaga agacctcttt taatttcagc cgttttgtcc gccacgatgc tttcaagcgt
    28201 tggcggtggt gcggaaaaag ggccgaatga aaagtggata atccccatca ttaaagcgat
    28261 ggcaccgaat agcatgccca atgaactgat tttattttgc gctatgatac ccatctgacc
    28321 tccttgtagc ggatggcaaa agcatatcag gaaacgggcg gacaaaattc ttaaaaaaaa
    28381 ccgctaaaga tagagcgtta gtgaaattgc aaaggagcaa caatgaaaat ttcccgcctc
    28441 ggagaagcgc ctgattaccg cttctcgctg gctaatgagc gtacttttct ggcgtggatc
    28501 cgtactgcac taggatttct ggcggcgggg gttgggcttg atcaacttgc gccagatttc
    28561 gccacgcccg tcattcgcga actgctggcg ttgttgttgt gtctgttttc cggcgggtta
    28621 gcgatgtatg gctacttgcg ctggttacgc aatgaaaaag cgatgcgtct gaaagaggac
    28681 ctgccctaca ccaacagctt attaatcatc agcttaatat tgatggtcgt agcagtgatc
    28741 gttatgggac tggtgttgta tgccggatag ccgcaaagcc agacgcattg ccgacccagg
    28801 gctgcagccg gagcgtacat cactggcgtg gtttcgtacc atgctgggct acggcgcgtt
    28861 gatggcgttg gctatcaaac acaactggca ccaggcgggc atgttattct ggatttcgat
    28921 tggcatcctc gccatcgtgg cgctgatcct ctggcactac acccgtaatc gcaatttaat
    28981 ggatgtcacg aatagcgatt tttcccaatt tcacgtagtc cgtgacaaat ttttgatctc
    29041 cctcgcggtg ttatctctcg caatactgtt tgctgtaacg catatacatc aacttatcgt
    29101 atttattgag aagagagtcg catgacagga agtcaggtca ttgatgctga agaggacagg
    29161 cataaactgg ttgtagagta taaggatact ctgcaacctg ccgattttta tcacaatttc
    29221 aaacagcggg gcatccgctc cgtacagctt attccccatc ttgaatttga tgaccggggc
    29281 gatatgacgg ccgcttcggt gacagcagag ctgtggggga aatttttaat tgccctgttc
    29341 gagtgttggg tgcgggcaga tatcagtcgt atctcgattg agctttttga ctccacttta
    29401 caaaaatggt gtggcagcga aaacccgcaa tcgcgccgtg actgccaggc gtgcgactgg
    29461 catcggctgt gtccgcacgc tcgggaggag acgccagaca gcatgctatg tgcgggctat
    29521 caggcgtttt attcttactc cgcaccgcat atgcgggtga tgcgtgatct gataaagcag
    29581 catcgttctc ccatggagct gatgacgatg ctgcggtgat gtgcgcttaa acgggctgcc
    29641 cctgatgcga catccgcgtt gccagcggta gccagcacag cacgatcaac aatcccatta
    29701 aggtcatcaa caaccccagg ctgccctgac cggtttgcgg caacatcgct gagagcgacg
    29761 ccagcacgcc ggaaccaatg ttttgcagac cgccgaccag cgcgccagcc gtgcccgcca
    29821 ggaaggggaa cggctccatc gcgccgctgg tcgccagcgg aaacagcatc ccggcaccga
    29881 aaaagaacag cgcggcggga acaagcagcg tccagacatt catcacgcca aaccagtcgg
    29941 ggatccacat cagcaagcca gccagcaggc agcagataac cgactgccac atcaacgttg
    30001 agaagcgttt attaggacgc ccggcaaacc atgccccaaa aaatgccgcc ggaatcggca
    30061 gaataaacaa aatactgacc gtcatactgc tcagccctaa caccgcgccc atcagcacgc
    30121 cggagcaggc ttcaaaggcg gcaatcccgg ccagaccgcc aatcagcatc agcaaataac
    30181 agttaaaacc gccgttaccg aaaagcgttt tataactggt aagcaggcgc gtgcgcggtg
    30241 catcgaccgg acgcgtttcc ggcatccagc gggccatact gaaggtcaca ccagcacaaa
    30301 gaaccaacaa aaagagataa caggcgcgcc agttccacat cgtatccagc agaccgccga
    30361 ttagcggtgc gagcaacgga ctaacaagaa tccccatgtt taacaggctg ttagcatggc
    30421 gcaactgtgt ccgttcatat aaatcacgcg gcaaagtacg cgccattacg ccgccaacgc
    30481 cggtgcccat cccctgcatc gcgctggcgg caatcaatac cgtcaaactg gaggtcgtga
    30541 ccgcgaccag cgttgccagc ataaaaatgg acattccgac gaggatcact ggtcggcgac
    30601 ccacgcggtc ggatatcggg ccataaaaca gctgcgagac accgtaagtc agcagataag
    30661 cgcccattac gctctgcacc gccccttcac ggacattgag atcgcgcgcc atatcggcaa
    30721 tagctggaat ataaatggtt tgcgccatct gaccgacggc cacgagtaat accaacatca
    30781 ataacaaatt gacgtttcta tgccttttca ttatcacgga tgcttttata aaaataatta
    30841 gaataagtca ctacgcattc ccataaatgc gggagaggaa tctatcacat tgataaatgt
    30901 tagccagata tacgccagga atggggaatt gtttagcggc agaatatgta aacaaaagcg
    30961 gcaataaatg ttgccggaag ggatggtagg tcggagaaga tgcgtcagca tcgcatccga
    31021 caccaactca cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact
    31081 tgaatctgaa ttatttcagg tatttcagaa cagcatcaag tagttgcagt gctgcaacaa
    31141 tgagtttgag gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag
    31201 gagcactgcc agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat
    31261 ctcaaggaga aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg
    31321 ctgacggcac cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc
    31381 ataacacatt gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt
    31441 agacaaaacg taaactgtga tcgatactca actatgtact aaatacgtca aaattcgtgc
    31501 cgaaattgcg cgttctgcgc ggaacaggta tactttcagt gttgacataa tacagtgtgc
    31561 tttgcggtta ccagccgcag gcggctgacg aaacctcgct ccggcggggt tttttgttac
    31621 ctgcaattca gtataaaacg tgatcaaccc ctcaattttc ccttgctgaa aaattttcca
    31681 ttgtctcccc tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc
    31741 cccaatgact acttccatgt tcaacgcaaa gctactacca actgcgccat ccgccgcagt
    31801 ggtcgtcgtg cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac
    31861 gattccaaaa ccccgccggc gcaaaccggg tggggttttt cgtttaagca cctcccggga
    31921 agtcggccca gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa
    31981 gcgctttacc ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt
    32041 gacgggcatt ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca
    32101 aatccgccat attctggctc gccatgaaca gggcgcgggg tttatcgctc agggaatggc
    32161 gcgcaccgac ggtaaaccgg cggtctgtat ggcctgtagc gggccggggg cgactaacct
    32221 ggtgaccgcc attgccgatg cgcggctgga ctccatcccg ctgatttgca tcactggtca
    32281 ggttcccgcc tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc
    32341 tatccccatc accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat
    32401 gagcgatgcc ttccgcattg cgcaatcagg ccgccctggc ccggtgtgga tagacattcc
    32461 taaggatgta caaacggcag tttttgagat tgaagctcag cccgcggtgg cagaaaaagc
    32521 cgctgcaccc gcctttagcg aagaaagcat tcgtgacgcg gctgcgatga ttaacgccgc
    32581 caaacgcccg gtgctttatc tgggtggcgg tgtgatcaat gcgcctgtgc gggtgcgtga
    32641 actggcggag aaagtgcaac tgcctactac catgacctta atggcgctgg gtatgttgcc
    32701 aaaagcgcat ccgctgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcactaacta
    32761 tatcttgcag gaggcggatt tactgattgt gctcggtgca cgttttgatg accgggcgat
    32821 tggcaaaacc gagcagttct gtccgaatgc caaaatcatt catgtcgata tcgaccgtgc
    32881 agagctgggt aaaatcaagc agccgcacgt ggcgattcag gcggatgttg atgacgtgct
    32941 ggcgcagttg atcccgctgg tggaagcgca accgcgtgca gagtggcacc agttggtagc
    33001 ggatttgcag cgtgagtttc cgtgtccaat cccgaaagcg tgcgatccgt taagccatta
    33061 cggcctgatc aacgccgttg ccgcctgtgt cgatgacaat gcaattatca ccaccgacgt
    33121 aggtcagcat cagatgtgga ccgcgcaagc ctatccgctc aatcgcccac gccagtggct
    33181 gacctctggc gggctgggca cgatgggctt tggcctgcct gcggcgattg gcgcggcgtt
    33241 ggcgaatccg gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat
    33301 tcaggagatg gcgaccgcca gtgaaaacca actggatgtc aaaatcattc tgatgaacaa
    33361 cgaagcgctg gggctggtgc atcagcaaca gagtctgttc tacaagcagg gcgtttttgc
    33421 cgccacctat ccgggcaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac
    33481 ctgtgatttg aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccg
    33541 tggcccggcg ctgatccatg tgcgtattga tgccgaagaa aaagtgtacc cgatggtgcc
    33601 gccaggtgcg gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcatga
    33661 caacgtgatt ctggaactca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg
    33721 cctttttgcc cgtcgcgctt ttaacgttga gggcattctt tgcttgccga ttcaggacag
    33781 cgacaaaagc cacatttggc tactggtcaa tgacgaccag cgtctggagc agatgataag
    33841 ccagatagac aagctggaag atgtggtgaa agtgcagcgt aatcagtccg atccgacgat
    33901 gtttaacaag attgcggtgt tttttcagta aggagcgact ttaaaccgct cggggggtac
    33961 tgatgagcga tttttgatag atccgttcag atatataggg tgaaattgtg ccagagacca
    34021 tctcaggcac aatttattag ccctgaatgt taatttaagt cgctgcacca gtaatttggg
    34081 ggttcacagt tagggctaaa atttccagcg atggtgttaa attctgattg ctgtctatac
    34141 caatggtcat gaccagcatt ctattaatta atggttcatc gtgaggtata ttatggcaag
    34201 ccctataaac agcggaataa tgatgtcaaa tctctgctct tccacctttt tggataaaaa
    34261 tataaatatc gctgctgagc ttgatattac aaataacgag aaaaaatatt cgcccggcag
    34321 taattttgca aaatggatgt tgcaggaaat aaagcgctta atagcaaata ttatgtccgg
    34381 ttctcgcagt gttaacaccg aaatccttaa ctatttccac cctatgccag gcactgaaaa
    34441 taatggcaat cgtacctgga tggcggcaac tggagagaac gaatacaccg taattgaaca
    34501 aacaggtggc aaatccttta atgtttttca ggttggtgca ggtaaaaata acataaaaga
    34561 aatattgggg aatacatcat attctggagt ggctgtagcg actattatca aatcgctgtc
    34621 tgaaaaaact gctgcgttag aaacacattc tgctgacacg gttttgagga aaaaattagt
    34681 taatagtatt gttatgatga atacggattg tagttacgaa atgcctgcag acattgtgag
    34741 taatacatat gatttattaa acctgcgaat tcaaaaagat gaggggcatt tacctgttca
    34801 gga
//
LOCUS       DAPGRN010000043        34718 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPGRN010000043 DAPGRN010000000
VERSION     DAPGRN010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.043"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            249..941
                     /locus_tag="RHW33_002236"
     CDS             249..941
                     /locus_tag="RHW33_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV1645734.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VINHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
     gene            964..2391
                     /gene="mdtD"
                     /locus_tag="RHW33_002237"
     CDS             964..2391
                     /gene="mdtD"
                     /locus_tag="RHW33_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV1645735.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASATMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="RHW33_002238"
     CDS             complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="RHW33_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV1645736.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="RHW33_002239"
     CDS             complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="RHW33_002239"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV1645737.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="RHW33_002240"
     CDS             complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="RHW33_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV1645738.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="RHW33_002241"
     CDS             complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="RHW33_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV1645739.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="RHW33_002242"
     CDS             complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="RHW33_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV1645740.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="RHW33_002243"
     CDS             complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="RHW33_002243"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV1645741.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8392..10260)
                     /gene="kup"
                     /locus_tag="RHW33_002244"
     CDS             complement(8392..10260)
                     /gene="kup"
                     /locus_tag="RHW33_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV1645742.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10483..11979
                     /gene="ravA"
                     /locus_tag="RHW33_002245"
     CDS             10483..11979
                     /gene="ravA"
                     /locus_tag="RHW33_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV1645743.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11973..13424
                     /gene="viaA"
                     /locus_tag="RHW33_002246"
     CDS             11973..13424
                     /gene="viaA"
                     /locus_tag="RHW33_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV1645744.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRNRLKDARVPQELTEEVMCYQQSQLLSTPQFIVQLPQIMDLLHRL
                     NSPWAEQARQLVDTNSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTLVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="RHW33_002247"
     CDS             complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="RHW33_002247"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV1645745.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14574..15032
                     /gene="asnC"
                     /locus_tag="RHW33_002248"
     CDS             14574..15032
                     /gene="asnC"
                     /locus_tag="RHW33_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV1645746.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15122..15565
                     /gene="mioC"
                     /locus_tag="RHW33_002249"
     CDS             15122..15565
                     /gene="mioC"
                     /locus_tag="RHW33_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV1645747.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15944..17833
                     /gene="mnmG"
                     /locus_tag="RHW33_002250"
     CDS             15944..17833
                     /gene="mnmG"
                     /locus_tag="RHW33_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV1645748.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17897..18520
                     /gene="rsmG"
                     /locus_tag="RHW33_002251"
     CDS             17897..18520
                     /gene="rsmG"
                     /locus_tag="RHW33_002251"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV1645749.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            19137..19517
                     /gene="atpI"
                     /locus_tag="RHW33_002252"
     CDS             19137..19517
                     /gene="atpI"
                     /locus_tag="RHW33_002252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV1645750.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            19526..20341
                     /gene="atpB"
                     /locus_tag="RHW33_002253"
     CDS             19526..20341
                     /gene="atpB"
                     /locus_tag="RHW33_002253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV1645751.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            20388..20627
                     /gene="atpE"
                     /locus_tag="RHW33_002254"
     CDS             20388..20627
                     /gene="atpE"
                     /locus_tag="RHW33_002254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV1645752.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            20689..21159
                     /gene="atpF"
                     /locus_tag="RHW33_002255"
     CDS             20689..21159
                     /gene="atpF"
                     /locus_tag="RHW33_002255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV1645753.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            21174..21707
                     /gene="atpH"
                     /locus_tag="RHW33_002256"
     CDS             21174..21707
                     /gene="atpH"
                     /locus_tag="RHW33_002256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV1645754.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            21720..23261
                     /gene="atpA"
                     /locus_tag="RHW33_002257"
     CDS             21720..23261
                     /gene="atpA"
                     /locus_tag="RHW33_002257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV1645755.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            23312..24175
                     /gene="atpG"
                     /locus_tag="RHW33_002258"
     CDS             23312..24175
                     /gene="atpG"
                     /locus_tag="RHW33_002258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV1645756.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            24202..25584
                     /gene="atpD"
                     /locus_tag="RHW33_002259"
     CDS             24202..25584
                     /gene="atpD"
                     /locus_tag="RHW33_002259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV1645757.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            25605..26024
                     /gene="atpC"
                     /locus_tag="RHW33_002260"
     CDS             25605..26024
                     /gene="atpC"
                     /locus_tag="RHW33_002260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV1645758.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            26375..27745
                     /gene="glmU"
                     /locus_tag="RHW33_002261"
     CDS             26375..27745
                     /gene="glmU"
                     /locus_tag="RHW33_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV1645759.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLEGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALVISRVPQTQKEGWR
                     RPVKKK"
     gene            27907..29736
                     /gene="glmS"
                     /locus_tag="RHW33_002262"
     CDS             27907..29736
                     /gene="glmS"
                     /locus_tag="RHW33_002262"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV1645760.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVGSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            30005..31045
                     /gene="pstS"
                     /locus_tag="RHW33_002263"
     CDS             30005..31045
                     /gene="pstS"
                     /locus_tag="RHW33_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV1645761.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNSVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVLPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            31131..32090
                     /gene="pstC"
                     /locus_tag="RHW33_002264"
     CDS             31131..32090
                     /gene="pstC"
                     /locus_tag="RHW33_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV1645762.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            32090..32980
                     /gene="pstA"
                     /locus_tag="RHW33_002265"
     CDS             32090..32980
                     /gene="pstA"
                     /locus_tag="RHW33_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV1645763.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            33071..33844
                     /gene="pstB"
                     /locus_tag="RHW33_002266"
     CDS             33071..33844
                     /gene="pstB"
                     /locus_tag="RHW33_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV1645764.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            33859..34584
                     /gene="phoU"
                     /locus_tag="RHW33_002267"
     CDS             33859..34584
                     /gene="phoU"
                     /locus_tag="RHW33_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV1645765.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDPKE"
BASE COUNT         8503 a         8850 c         8971 g         8394 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttctg
      121 accgcgcatt ttttcagcaa aagccccgtt aatgtgccat aaaacaagca atttgccgtt
      181 tttgcaaccg taatcacact tcctggtggc atactaagga ggaataaaaa gaaaaaggaa
      241 gataagtaat gcctttaagc gcacaacagt tagctgcaca gaaaaacctt tcgtatgttc
      301 tggctgagaa gctggcgcaa cggatcttaa aaggtgaata tgaacccggc accattttgc
      361 ctggtgagat tgagctgggc gagcaatttg gagtgagtcg tacagcggta cgcgaagcgg
      421 tcaaaacgtt aacggcaaaa gggatggttt taccgcgacc gcgaattggt acccgggtca
      481 tgccacaatc gaactggaat tttcttgatc aggaattgct tacctggtgg atgacagaag
      541 agaactttca tcaggtcatc aatcactttc tggttatgcg tatctgtctg gagccacaag
      601 cttgcctact ggcagcaacg gttggcaccg cggaacagaa agcgcatctc aatacgttaa
      661 tggccgaaat ggcggcatta aaagagaact ttcgccgtga acgctggatc gaagtcgata
      721 tggcctggca tgagcatatc tatgaaatga gcgccaatcc gtttttgacc tcattcgcct
      781 cgctattcca ttcggtttat cacacttact tcacgtcaat taccagcgac acagtgataa
      841 agctggatct gcatcaggcg atagtcgatg cgattatcca aagcgatggc gacgcggcat
      901 ttaaagcttg tcaggcgctg ctacgctcac ctgataagtg ataacccgat aacaggacat
      961 tgaatgagcg ataaaaagaa gcgcagtatg gcgggtttgc cgtggatcgc ggcgatggcc
     1021 ttcttcatgc aggcacttga tgccactatt ctgaataccg ccttacccgc aatcgctcat
     1081 agccttaatc gttctcctct cgcgatgcaa tcagccatca tcagttatac gctgacggtg
     1141 gcgatgctta ttccggtaag cggatggcta gccgatcgct tcggtacgcg tcgcattttt
     1201 acccttgccg tgagtctgtt cacattgggt tctctggcct gcgcactttc taattcgcta
     1261 ccacagctgg ttgtcttccg ggttattcag gggataggcg gcgcaatgat gatgcctgtt
     1321 gctcggctgg ccttactgcg cgcttatcct cgtaatgaac ttcttccagt attgaatttt
     1381 gtcgccatgc cgggtctggt ggggccaatt ttaggccccg ttcttggcgg cgtgctggtc
     1441 acctgggcaa cctggcactg gatattttta atcaatatcc ccataggtat tgcaggcctt
     1501 ctttacgcgc gcaaacatat gcccaatttc accaccgcac gacgcagatt cgatatcact
     1561 ggctttttgt tgtttggcct cagccttgtt ctcttctcaa gcggaataga gctattcggg
     1621 gaaaagattg tcgccagctg gattgccttg acggtaattg tcaccagcat cgggttactg
     1681 cttctctata ttctccatgc acgacgcacg ccaaacccat taatttcatt agatttattt
     1741 aaaacccgca ctttctcgat cggtatcgta ggcaatattg caacccgtct ggggaccggc
     1801 tgtgtaccgt tccttatgcc attgatgtta caggtaggat ttggttatca ggcgtttatt
     1861 gctggctgta tgatggcacc gacagcgtta ggttccatta ttgcaaaatc gatggttacc
     1921 caagtcttac gtcgtctggg ctatcgccat acgttagtgg ggatcacggt gattattggg
     1981 ctaatgatcg ctcagttctc tttgcaatca ccggcaatgg cgatatggat gctgatcttg
     2041 ccgttgttta tattagggat ggctatgtcg acgcagttta ccgcaatgaa taccatcacg
     2101 cttgccgacc tgaccgatga caacgccagc agcggtaaca gtgttctggc ggtcacgcag
     2161 caactgtcga tcagtttagg cgttgctgta agtgcggccg tccttcgcgt ttatgaaggg
     2221 atggaaggca caacgactgt cgaacaattc cactatacgt ttatcacgat gggcattatt
     2281 actgttgctt cagcaacaat gttcatgctt ctgaaaacaa ccgatggtaa taatttgatc
     2341 aaaagacagc gtaaatctaa gccgaaccgc gttccatcag aatcggagta agttgtaatc
     2401 gttgttgctg aagagttggc tgggttatcc gatggatgag tacatcaatc gccagctccc
     2461 ccagttcatc tttcggttgg tggatagtgg ttaatggtgg cgtcataaag cttgccagtt
     2521 cgatatcgtc atagccaatc accgcgatat cctgcggaac ctgtaactct gcctgatata
     2581 acgcctggta aacgccaaca gccatagcgt catttccggt aaagacggcc tgaggacgca
     2641 gcggatgtga tagcagttgg cgcatagcgt caaacccacc gttaaattca aaatcaccag
     2701 tgacttcata gccatcagga atgttgagac ccgcacgttt catcgccgcc cgataacctt
     2761 ccaaccgcag gcgcgccgga gttttatcca gcgggccggt aatacaggcg atacgggtat
     2821 gacctttatc gatcagatat tgcgttgcta agtctccgcc cagcaacgag ttatcctgaa
     2881 taagatcgct gtcgccatcg aacggagccc agtccatcat cacggtaggc actgtcggat
     2941 aacgttgcat gatttcacgc gaaggctgat gcgtttcggt gcacagtaac agcaagccat
     3001 caacgcgttt ttgcatcagc gtttccagat tgcgattcat ccgctgttca tcgccttcgg
     3061 tattgcaaag gacgagacta taaccgcgtt cgaagcagct gcgttcaacg ccacgcacca
     3121 gttctgaata gaaaggattg gtactggcag tgatcaacat gccaatggta tgtgtttgat
     3181 tgagtttgag gctacgcgcc agagctgatg gcgcgtaatt gagttcttta atcgccgctt
     3241 caactttggc ggtaatcgct tcactgacga agcgatcttt attgataacg tgagaaactg
     3301 ttgaggtaga aacgcccgcc aggcgggcaa catctttcat tgtagccaag cgtcacctct
     3361 gcctgtctaa aaatgcgtcg atctcttcac gccacggtac ggaaggttgt gcgcctttac
     3421 gtgttacggc aatcgcagcg gcagcatggg caaaccgaat cgcctctggc aatggttttt
     3481 cttccagcaa cgccgtgatt aacgcgccgt taaaggtatc tccggcagca atggtatcga
     3541 cagcctgcac ccggaatcca ggaacgcgct gaccttcacc attcacgcta gcccatacac
     3601 cacgacttcc taaagtaatc agtacagtac ggataccttt ttcatgcagt acctgcgccg
     3661 ccttcgctgc atcttcatca ttttcaacac gaataccggt gagcttttct gcttccgttt
     3721 cgtttggcgt aataatgtcc accagcgcca gcagttcgtc aggaagttcg cgagccggag
     3781 ccgggttaag cgcaacgata gtcttatttt gatgggcgat tttcgccgct gccatcacac
     3841 tttcgagtgg tgattccagc tgcattaata atgctgacgc gttggcaata cgctcacgtt
     3901 gcgcttccac cagcgccggg gaaagggcag cattagcgcc ggcatgaata ccaatgacat
     3961 tctcaccttc gccattaaca aaaatcagcg ccacacctgt tgattcgcct ttgatcacgc
     4021 tgaccggagt aatatcgatg ttatcagtgg cgagctgctg gcgaacgctc tcaccaatgc
     4081 tgtcatcgcc agtgcaggca ataaacgcga tattcgcacc gctacgccca gcagccacag
     4141 cctgattcgc gcctttgccg ccaaatgcaa cctgatagtg gttaccggtt acggtttcgc
     4201 ctggagtagg aaaagattga agattaagaa tgtggtcagc attaatgctg ccaagaacaa
     4261 cgaggctgcc tgcgttttgc atattcggga tgtccataaa atgcgccacc gtgttagggt
     4321 ggcgcgtgcc ctgcttttct ttatattaca cgttcatctg tcgatgacgt atttatgtca
     4381 ccatcaggtc atacaacctg attaaaacta ctgcttaaca accagtttta gatcaaccgg
     4441 atacttagcc tgaactttct cgcctttcag cactttatct gcggtttcaa cgcctttcgc
     4501 gccaatctga tcgggtagct gagcgatagt cgctgctagt ttgccatcat tcaccgcttt
     4561 ttcgccatcc ggtgtaccgt caaatccgac gaccatcaca tccgatttac cggcagtttg
     4621 cagtgcgcgc agcgcgccca gcgccatttc atcattctgc gcaaatacag cctgaacatc
     4681 cggatgagcg gtcaacaggt tctgcattac gttcaaacct ttggtgcgat caaaatctgc
     4741 tggctggctg gcaagaacat taaacttgtg agcagctacg gcctgctgga agccttcgcc
     4801 acgttcacgg gctgcggatg taccagcaat gccttgcagc tcgataactt tggcaccttc
     4861 acccgctttc ttcgcgatgt aatcaccggc gattttgccg cccagtacgt tatcagatgc
     4921 aatgtggctc accacttcac ctttcgttgc ctggcggtca agagtgataa ccgggatgtt
     4981 cgcctggtta gccatcttca cagcattacc cactgcgtcg gagtcggtcg ggttaatcag
     5041 cagaattttt gtgccgcgaa cggttaagtc ctgcacgttc gccagctctt tcgccgggtt
     5101 gttctgggag tccagcacca ccaggttata gccaagttta tccgcctctt tctgcgcgcc
     5161 atctttcagc gatacaaaga acgggttgtt aagcgtggag accaccagcg cgatggtgtc
     5221 ttttgccatc gcattcgcac tgacggtggc gcttagcgca acagcggaaa ccagggtagc
     5281 cagttttttc atgttcatat tcaagatgtc ctgtagtcgt tattactgct ttttgttgtc
     5341 taccagcacc gccagcaaaa tcaccaccgc tttgacgatc atctggtaat aggaggaaac
     5401 acctaacaaa ttcaatccat tattaaggaa gccaagaatt aatgcgccga tcaacgtccc
     5461 aacaatgcga ccttttccgc ccgccagact cgtaccgccc agaaccaccg cagcaatagc
     5521 atccagctca tagccagtcc ccgccgtggg ttgtgcggag gagagacgcg ccacttcaat
     5581 aatcccggcc agcgatgcca gcagaccaca aagagaatag acgatgattt tgattttatt
     5641 gacgttgata ccagaaagac gcgttgccgc ttcgttgccg cccagcgcgt agatgtaacg
     5701 ccccagacgc gtgtgatgca gcatgtacca ggccgcgagg aagacaatcc ccatgatcca
     5761 gactggcgtc ggtacaccca gcggacgacc aataccaaac cagccaaaca gatcggcgtt
     5821 ctcagtaaag ccggtattca ctgggctacc gttggtataa accatggtca cgccgcgcag
     5881 taaaagcatc ataaccagcg tagcgataaa cgcctggacg cgacctttcg ctacaatcac
     5941 cccggttacc gcaccaattg cggcacctaa cgcgagagca gcagcgacag ccaccagcgc
     6001 attgacttca atgccgacga tagatgcagc aactgcgccg gttagcgcca acagagaacc
     6061 taccgacaag tctatgcccg acgtcaggat caccagcgtc atcccgaccg ccataatggc
     6121 gttcaccgaa gtttgctgga gaatattgaa taagttattg atggtgaaaa agttcggact
     6181 taacgtcgag acaatcgcga tcagcaccag cagagcgata agcgatttct gctccatcag
     6241 ccacgctttc gtgaaataac ggcgaccaga gacagtctgg gttgtcattt ttttactcct
     6301 gattcacgcg attaagcttg cccacagcgg cagccattaa cacttcctgg gtggcctgct
     6361 cacgagtaaa ttccccgctg agatgccctt catgcatgac gatgatgcga tcgctcatgc
     6421 ctaatacttc tggcatctcc gatgacacca gaatgatgct caagccatcg gctttgaact
     6481 ggttaatcag ttgatagatc tcttttttcg cgccgacatc tacgccacgg gtaggctcat
     6541 cgaggatcaa caccttgggg cgtgtcatca gaccgcgggc aatcgccact ttttgctgat
     6601 tgccaccgga aagcagacca attgcctgtt ccatcgacgg cgttttcaca ttaaacagac
     6661 gaatgaaatc acttactgcc tgctgttcat cggcatgctt caaactgccg ccagcgcggc
     6721 tgaagtagcg cagcgctgtc agcgacatgt tctcttttac tgacatgccc aacactaaac
     6781 cgtcacgttt acggtcttcg gagatataca caatgccgtt tgccaggcca tcctgcggtg
     6841 aacgggtaac gacttcatgc ccatccaggg tgacgtaacc gctggtgcgc ggtagtgcgc
     6901 cgtagagcac tttcatcagt tcggtacgac ctgcgcccat caaaccagag acgccaagaa
     6961 tttcgccttt gcgtaaagta aaagagacat cgttaacgcc aggtccgcag agattatcga
     7021 ctttcaggcg gatatctccc ggcgctttgt ccaggtgcgg atattgatct tccagcttgc
     7081 gacccaccat catctcaatc agcgaatctt cggtcagtga tgccacttcg cgctcagcaa
     7141 taaattgtcc gtcacgaaaa acggtaacgt catcgcaaat ctcgaagatt tctttcatac
     7201 ggtgggagat atagacaata ccgcggcctt gcgatttcag ctcgcggatg acgcggaaca
     7261 gggattcggt ttcggtatcg gtcagcgcat cggtcggttc atccataatg atgactttcg
     7321 actcaaagct cagcactttg gctatttcaa ccatttgctg gtcaccgatg gaaagatcgc
     7381 ccaccagctt gtcgctttta aagcgcaggt taagtttagc cagcaattta tccgcttcgg
     7441 catacatggt tttccagtca attttgccaa agcgattaac aaactcacga ccgaggaaaa
     7501 tgttttcggc aatggttaac tgcgggatca ggttcagttc ctgatggata atcccaatcc
     7561 cggcttcctg ggaagatttc ggcccggtaa atgtcgtttc tttccccagc cataaaagcg
     7621 tgccggcatc gcgagtatag ataccagtaa gcactttcat catggtggat ttacccgcgc
     7681 cgttttcgcc caccagcgcc atcacgcggc ccggatagac atttaacgca gcgcccgaga
     7741 gggcttttac gcccgggaag gctttatcga tgcctttaag ctgaagtaat gcttccatga
     7801 cggcctcaga acgtcacgcc agcacagaga atgatattcg catacggaga acattctccg
     7861 ctgcgaatta ccgcctgact ttctgcggtt tgttgtttga attgttcatg cgtggtgtaa
     7921 cgaatttcaa tggtatttcc ctggtgtttt tgcagctgct caaggtgagt gagcaacgtt
     7981 tcgtggagtt gcggattatg ttgtttgatc tcttccgcga taatggccgc ctcgacctgc
     8041 atttcatttg tgacgacgcc cagcacctgc ataaaagaag gtacaccctg ggttaatgcc
     8101 atatcgatac gcgttgtact tttggggatg ggtaacccag catcacacac caccagcgta
     8161 tcggtatgtc ccagacggga gatcaccgat gaaatatcag aattaagaac ggtgcctttt
     8221 ttcatttttt tctccatcag cgaaacgttt cgctgacccg cagtttaatc ctgactgagt
     8281 tgggaaaacc atcgtgacgt tacggaaatg tgatcaacct cgaaacgttt tacatggtga
     8341 ttaaccatga aaacaaaaac gcccccttat aaagaaagga ggcgtctggc gttagatttc
     8401 gacctgagta cccagttcga taaccctgtt tggcgggatt tcaaattgat ctggtgcacg
     8461 cagcgcatta cgttgcagca gcaagtacag cttgccgcgc agacgcaaat accacgggcg
     8521 tttgccgagg atcaacgact catgcgacat aaagaaggag gtttccatca tccggcaact
     8581 taatccttcc agaccgcagc ggtggaaaac ttcttccacg tttggcgttt ctcgccaacc
     8641 ataacttgcc accacgcgcc agaaagtggg cgacagttgt tcaatctgta cccgacggac
     8701 gttatggaca tatggcgcgt cttcggtgcg cagagttaac agaatcaccc gctcatgcaa
     8761 taccttgtta tgtttaaggt tatgcatcag cgcaaaggga atgacgttga ttgcacgcga
     8821 catatacacc gcggtcccgg gcacgcgaac aggcggtgat ttctccagcg aagcaatcat
     8881 cgcttccaga gagttaccat gttcatgcat ccgccgcagc aagcggaaac gctcgctctt
     8941 ccaggtggtc atcacgataa acatcacagt accgaggctc aatggcaacc agccgccaga
     9001 gagcagttta tcgaggttag cggtgaacaa tggaatatcg acacaaagga aagcaatcag
     9061 gatcagcgca acaaaatact tattccagtg ccagttctga cgtgccacgg tagtcgagag
     9121 aatagacgtc agcaccatgg ttccggtcac cgcaatcccg tacgccgccg ccaggttgct
     9181 ggagtgctca aagctgacaa tcacaatcac gaccgcgaca tagagcatcc agttcacaaa
     9241 cggaatatag atttgccctg actccatttc ggaggtgtga ataatgcgca tcggcgacaa
     9301 atatcccaga cgtaccgcct gacgcgtcaa tgaaaagacg ccagagataa ccgcctgcga
     9361 ggcgattacc gtcgccagtg cggcgatgat cagcagcggg atcagcgccc agtccggtgc
     9421 caaaaggaag aacgggttct taatcgcttc cgggttcttt aacaacagcg ctccctggcc
     9481 gaagtaatta agagttaagg aaggtaatac gacagtgaac cacgccagac gaatagggaa
     9541 cttaccaaag tgtcccatat cagcatacag cgcctcgacc cccgtaatcg acagcaccac
     9601 tgcccctaat gcaataaaag aaaccgtttt gtattcaagg aagaaatgca ccgcccacat
     9661 cggattcagc gcatgcagca cttccgggtt agcaataatg ctacgtaacc ccagtcctgc
     9721 cagaatcaaa aaccaggtca gcatgatcgg cgcaaacagc ttaccgacca tagccgtgcc
     9781 atgcttttga atcataaata ataaagtgag aacgataatt gagaggggaa ctatccaggt
     9841 atccagctgc ggggcgacga tttccagacc ttcaatggct gacatcaccg aaatagcggg
     9901 tgttatgacg acttcaccat agaaaaagct gccgccgatt agccccataa tcaccagcat
     9961 tgatgtagtt cgcgccgacg tattacgccc ggcaagcgac atcaacgtca ggatcccccc
    10021 ttcaccggcg ttatctgccc gcatcacgaa ggtgagatat ttaatggaaa ccacaaagat
    10081 tagcagccag aagatcagcg ataaaaagcc aaacacggca tcgcgttcaa cgccaaaacc
    10141 aaactggccg gacaaacatt cacgaagtgt atataacggg ctggtaccaa tatcgccgta
    10201 gacaactcca atcgccgcga gggtaatcgc gggcaatgat tgcttattat cagtgctcat
    10261 agactagtct ttcgttgaaa tatgaaatgt gtgcttagtc ccttggccca caaaaaagcg
    10321 cacagtatgc acgattaacg gcaaaatcgt actcctaaat gcggccacat taacctggct
    10381 caaagaaaaa taccccccct ttgagactat tttagccacc agcaagaaac gtctatactc
    10441 gcaatttacg cagaactttt gacgaaagga cgccacttca ttatggctca ccctcattta
    10501 ttagcggaaa gaatttcccg cctgagcagt tcgttggaaa aggggcttta tgaacgtagc
    10561 cacgccatcc gcttgtgttt attagcggca ttaagtggtg aaagtgtgtt cctccttggc
    10621 ccgccaggta ttgccaaaag tttgatcgcc cggcgcttaa aattcgcctt tcagaatgcc
    10681 cgcgcgtttg aatatctgat gactcgcttc tccacgccgg aagaggtttt tggtcccctt
    10741 tctattcagg cgctaaaaga tgaagggcgc tatgaacgtt taaccagcgg ttacctgccg
    10801 gaagcagaaa tcgtctttct ggatgagatc tggaaagcgg gtccggcaat tcttaatacc
    10861 ctgctcaccg ccattaacga gcgccagttc cgcaacggtg cacacgtaga aaaaatcccg
    10921 atgcgtctac tggtggcggc ctccaacgag ctgccggaag cagacagcag tctggaagcg
    10981 ttatatgacc gcatgctgat tcgtctgtgg ttagataaag tgcaggataa agcgaatttc
    11041 cgctccatgc tgaccagtca acaggatgaa aatgacaatc cggttccagc ctccttgcag
    11101 gtcacagatg aagaatatga acgctggcag aaagagattg gtgaaattac gctgcccgat
    11161 catgtatttg agctgatttt tatgctgcgc cagcaactgg ataaattacc ggatgcgcct
    11221 tatgtctcgg atcgtcgctg gaaaaaagcg attcgattat tgcaggccag cgcctttttt
    11281 agcggtcgca gtgctgttgc cccggttgat ctcattttgc taaaagattg cctgtggtat
    11341 gacgcgcaaa gcctgaattt gatacaacaa caaattgatg tattgatgac cggtcacgcc
    11401 tggcaacagc aagggatgtt gactcgcctt ggcgcgattg tgcaacgtca cctgcaactc
    11461 cagcagcaac aaagcgataa aacagcctta acggtaattc gtctgggcgg cattttcagc
    11521 cgtcgtcagc agtatcaact tcctgttaac gttactgctt ccactctgac tctgctgctg
    11581 caaaaaccgt taaaattgca tgatatggaa gtggttcata tctcctttga gcgtaacgcg
    11641 ctggaacagt ggctgagtaa aggtggtgaa attcgcggca aactgaacgg tatcggcttt
    11701 gcccagaaat tgaatctgga agtggatagc gcccaacatc ttgttgtacg cgatgtaagt
    11761 ttacaaggca gtacgctggc acttcctggt tcatcggctg aaggtctgcc aggtgaaata
    11821 aaacaacaac tggaagagct tgaaagtgac tggcgcaagc aacacgcttt attcagcgaa
    11881 cagcaaaaat gtctgtttat ccctggcgac tggttaggtc gcattgaagc cagcctacag
    11941 gatgtcggcg cacagattcg ccaggcacaa caatgctaac gctggatacg cttaatgtga
    12001 tgctggccgt cagcgaagag ggattgatcg aagagatgat catcgcgctg ctggcctcac
    12061 cgcagctggc ggtcttcttt gaaaaattcc cacgactgaa ggcggcaatc actgatgatg
    12121 ttccccgctg gcgtgaggcg ctgcgtaatc ggctgaaaga tgcccgagtc ccgcaggaac
    12181 tcaccgaaga ggtgatgtgc tatcagcaaa gccagctcct ctccacgccg cagtttattg
    12241 tgcagctacc acagatcatg gacttactgc atcgtctgaa ttccccatgg gcagaacaag
    12301 cccggcagtt ggttgatact aacagcacga tcacttcagc gttacacacg ctttttctcc
    12361 agcgttggcg tttaagtctg atcgtgcaag caacgacgtt aaatcaacag ctattagaag
    12421 aagaacgcga acaactgttg agtgaagttc aggaacgcat gacgctgagc ggacaacttg
    12481 aaccgattct cgcagataac aataccgcag cgggtcgtct gtgggatatg agcgccggtc
    12541 agcttaaacg tggcgactat cagttgattg tgaaatacgg tgaatttctt aacgaacagc
    12601 cggaactgaa acgcctggca gaacagctgg ggcgttcccg ggaagccaaa tcaataccgc
    12661 gcaacgatgc gcagatggaa accttccgca ccctggtgcg cgaaccggcg acggttcctg
    12721 agcaggttga tggtctgcaa caaagcgatg atattttacg tcttctgccg ccagaactgg
    12781 cgacactagg gataacggaa ctggagtatg agttttaccg tcggctggtg gaaaaacagt
    12841 tgctcaccta tcgcctgcac ggtgagtcat ggcgtgaaaa agtgatcgaa cgcccggtgg
    12901 tgcataaaga ttacgacgaa cagccgcgcg gaccgtttat tgtctgtgtg gatacttccg
    12961 gctcaatggg cggctttaat gaacagtgtg cgaaagcgtt ctgcctggcc ttgatgcgca
    13021 ttgctctcgc tgaaaaccgg cgctgctata ttatgctatt ttccaccgag atcgtccgtt
    13081 atgagctttc tggcccacaa ggcatcgaac aagcaatccg ttttttaagc cagcagtttc
    13141 gtggcggcac cgatcttgcc agttgttttc gcgccattat ggaacgcttg caaagcaggg
    13201 aatggtttga tgccgatgcg gtggtgattt ctgattttat cgctcagcgg ttgcctgacg
    13261 acgtgacgag taaagtgaaa gagctgcagc gggtacatca gcatcgcttt catgccgtgg
    13321 cgatgtcggc acacggcaaa cccggcatca tgcgcatttt cgatcatatc tggcgctttg
    13381 ataccgggat gcgaagccgc ctgctcagac gctggcggcg ataaattatt acagcagaga
    13441 agggacgctc tcgcgaacag cagctggcca tactccacac tgaacctggc cgatatgcgg
    13501 cagttgcagc agcagcatgg tcaaacgaga ctggccgata ccgccgccga tggtctgcgg
    13561 catttcaccg cgcagcaacg cctgatgcca ttccagctgc agacgatctt catcaccggt
    13621 cagcgccaac tgatgcttca acgtgtcggc atccacgcgg atccccatgg aagaaagctc
    13681 aaacgcatct tccagtaccg ggttccacac cagaatatcg ccgttcaggc ccgcatggcc
    13741 cagctctgac ggggtgctcc agtcatcata atccggtgcg cgcacgtcgt gacgatgacc
    13801 atcactcaac ttgccgccaa tcccgacaag gaataccgca ccaagatctt tcgctatcgc
    13861 ccgctcacgc cctttggcat caagatccgg ataacgagac agtaactcct ggctgtgtac
    13921 gaagtggatc tgatccggca ggaacggtgc caggccaaac tcttcgctaa ccgcagcttc
    13981 ggttgcttta attcccgccc agatcgcctc taccgtgctt ttcagcgtcg agaattgacg
    14041 ctcaccgtcg cccattacgc gttcccagtc ccactggtca acatagaccg agtgcaacgg
    14101 agaaagacgg tcttcatcgg ggcgaagggc tttcatgtgc gtgtacagcc cttcgcccgc
    14161 gctgaagtcg tgttgcccta aggtctgacg tttccacttc gccagtgaat gaaccacttc
    14221 gaactgggca tcaggcagag ctttcacttt tacctgcacc gctttttcac agcccgacaa
    14281 gttatcctgc gtgccatccc ccacacggct aagaatcggc gcctggactt cgatcagccc
    14341 cagacgttct tccagttggc gagaaaagtg agatttcacg aagctaattt ggcgttgttt
    14401 ggcaatgtaa gcggttttca tttttttata ctcctgcgtc ctgttgctta tgattaagca
    14461 acaaaaaccg acacacatgc aattatcatt caataaaaag cctaacacac ttttgattca
    14521 ttaaaaaaga aggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa
    14581 attatctgat cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca
    14641 ccgcttacgc cgaactggcg aaacaatttg gcgtcagtcc agggacgatt cacgttcgag
    14701 tagagaaaat gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc
    14761 agctcggtta tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactatc
    14821 cttccgcgct ggcgaaactg gaaagcctcg atgaagtcac tgaagcctat tacacaaccg
    14881 gccactacag catctttata aaagtgatgt gccgttcgat cgatgctctc cagcatgtac
    14941 ttatcaacaa gatccaaaca attgatgaaa ttcagtccac cgagacactg atcgtcctgc
    15001 agaacccgat catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca
    15061 caggtagatc ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt
    15121 tatggcagat atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga
    15181 acacctggct gaaaagctgg aagaggcggg ttttaccacc gaaacgctgc acggtccgct
    15241 gttggaagat ttatctgcct cggggatctg gctggttatc agctcaaccc acggtgccgg
    15301 agatattccg gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct
    15361 ttctgcggtc cgctttggcg caatcggtat tggtagtcgt gaatatgaca ccttttgtgg
    15421 ggctattgat aaactcgagg ctgaactcaa aaattccggt gcaaaacaga cgggcgaaac
    15481 actgaagatc aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg
    15541 atcgtggatt aatttactca aatgagtata cagatcgtgc gatctactgt ggataacttt
    15601 gtcaggaagc ttggatcaac cggtagttat ccaaagaaca acggttgttc agtttttgag
    15661 ttgtgtataa cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac
    15721 agttaatgat cctttccagg ttgctgatct taaaagccgg atccttgtta tccacagggc
    15781 agtgcgatcc taataagaga tcacaataga acagatctct aaataaatag atcttctttt
    15841 taatacccag gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg
    15901 ggcttcaatc cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc
    15961 ttttgacgtc atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc
    16021 gcgtatgggt caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag
    16081 ctgcaacccg gcgatcggag gtattgggaa gggacatctg gtaaaagaag tggatgcact
    16141 cggcggtctg atggcgaaag cgatcgatca ggcgggtatc cagtttagga tactaaacgc
    16201 aagcaaggga ccggcagttc gcgctacccg agctcaggcg gatcgtgtgc tctaccgtca
    16261 ggcggtacgt acggcgctgg agaaccaacc gaatctgatg atcttccagc aggcggttga
    16321 agatcttatt gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt
    16381 ccgtgccaaa gccgtcgtgc tcaccgttgg gacgttcctc gacggtaaaa ttcatatcgg
    16441 tctggataac tacagcggtg gccgtgctgg tgatccgccg tccattccgc tttctcgccg
    16501 tttgcgtgaa ctgccgctgc gcgttggtcg tctgaaaacc gggacaccac cgcgtattga
    16561 tgctcgaacc atcgacttta gcgtactggc gcaacagcat ggcgataacc caatgccggt
    16621 attctcgttt atgggcaatg cgtcccagca tccccagcag gtgccgtgtt atatcactca
    16681 taccaacgag aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtacgc
    16741 aggggtgatc gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg
    16801 cttcgccgac agaaatcagc atcagatctt ccttgaaccg gaagggctga cctctaacga
    16861 aatttatccg aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg
    16921 ctctatgcag gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga
    16981 cttcttcgat cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt
    17041 ctttgctggt cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct
    17101 ggccggtctt aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcgcgttc
    17161 tcaggcgtat ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta
    17221 tcgtatgttt acctcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct
    17281 gcgtttgact gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt
    17341 taacgagaaa cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac
    17401 cccgtcggcg gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga
    17461 agccagtggt gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct
    17521 gacgccgttt gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt
    17581 taaatacgaa ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga
    17641 gaacaccttg ctacccgcga cactggatta ccgccaggta tccggtcttt ctaacgaagt
    17701 gatcgccaaa cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg
    17761 cgtcacgcct gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg
    17821 tcgtagcgca taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca
    17881 agaacaggta atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc
    17941 gcttaccgat caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa
    18001 caaagcgtac aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct
    18061 cgatagcatt gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg
    18121 tcctggactg cctggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt
    18181 ggatagcctt ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga
    18241 gaatattgag ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg
    18301 cgtaattagc cgcgcttttg cctctctgaa cgatatggtg agctggtgtc accatcttcc
    18361 tggtgagcaa ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt
    18421 gttgcccgaa gaatatcagg ttgaatcagt ggttaaactt caggttcctg ccctggatgg
    18481 cgaacgccat ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata
    18541 aaaaattgac cggttagact gttaacaaca accaggtttt ctactgatat aactggttac
    18601 atttaacgcc acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca
    18661 gaaaataagt cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt
    18721 ggctgttaaa acattattaa aaatgtcaat gggtggtttt tgttgtgtaa atgtcattta
    18781 ttaaaacagt atctgttttt agactgaaat atcataaact tgcaaaggca tcatttgcca
    18841 agtaaataaa tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca
    18901 ccagtgttta cgcgttattt acagtttttc atgatcgaac agggttagca gaaaagtcgc
    18961 aattgtatgc actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg
    19021 aaatcacggg ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa
    19081 agaaagtttt atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt
    19141 ctgtgtcgct cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg
    19201 tgatagcaag tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa
    19261 gcgggggcct ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc
    19321 aggcgcatac accagcgaaa ggccgagtgg cctggacatt cgcatttggc gaagctttca
    19381 aagttctggc gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct
    19441 tgccgctgat cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa
    19501 ttaacaacaa agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat
    19561 aggacaccac ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca
    19621 aaacccccca gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct
    19681 gggtctgttg ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc
    19741 aggtaagttt cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga
    19801 catgtaccat ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt
    19861 attcctgatg aacctgatgg atttactgcc tatcgacctg ctgccgtaca ttgctgaaca
    19921 tgtactgggt ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc
    19981 tatggcactg ggcgtattta tcctgattct gttctacagc atcaaaatga aaggcatcgg
    20041 cggcttcacg aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa
    20101 cttaatcctt gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt
    20161 cggtaacatg tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg
    20221 gtcacagtgg atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca
    20281 agccttcatc ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta
    20341 atttaccaac actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga
    20401 atatggatct gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg
    20461 cgatcggtat cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc
    20521 tgattcctct gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga
    20581 tgatcgctgt aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt
    20641 tatttaaaga gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac
    20701 gcaacaatcc tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac
    20761 gtatggccgc cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt
    20821 gcttccgcag aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag
    20881 ctgaaaaaag cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg
    20941 cagattctgg acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag
    21001 gcgcaggcag aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt
    21061 gctatcctgg ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct
    21121 aacagcgaca tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg
    21181 aatttattac ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc
    21241 aaagtgtaga acgctggcag gacatgctgg cgtttgccgc cgaggtaacc aaaaacgaac
    21301 aaatggcaga gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg
    21361 cagtttgtgg tgagcaactg gacgaaaacg gtcagaacct gattcgggtt atggctgaga
    21421 atggtcgtct taacgcgctc ccggatgttc tggagcagtt tattcacctg cgtgccgtga
    21481 gtgaggctac cgctgaggta gacgtcattt ctgctgccgc actgagtgaa caacagctcg
    21541 cgaaaatttc tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa
    21601 tcgataagtc tgtaatggca ggcgttatca tccgagcggg tgatatggtc attgatggca
    21661 gcgtacgcgg tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca
    21721 tgcaactgaa ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg
    21781 ttgtgagtga agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca
    21841 ttcacggcct ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg
    21901 ctatcgcact gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtatgctg
    21961 accttgccga aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc
    22021 gtggcctgct gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc
    22081 tggatcacga cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt
    22141 ccgtagatca gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc
    22201 gtggtcagcg tgaattgatc atcggtgacc gtcagaccgg taaaaccgca ctggctatcg
    22261 atgccatcat caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga
    22321 aagcgtccac catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca
    22381 ccatcgttgt ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg
    22441 ccggttgcgc aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg
    22501 atgacctgtc taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc
    22561 caggacgtga agcattcccg ggcgacgtat tctacctcca ctctcgtctg ctggagcgtg
    22621 ctgcgcgtgt taacgccgaa tacgttgaag ctttcaccaa aggtgaagtg aaagggaaaa
    22681 ccggttcact gaccgcgctg ccgattatcg aaactcaagc gggtgacgtt tctgcgttcg
    22741 ttccgaccaa cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca
    22801 acgccggtat tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag
    22861 cacagaccaa gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc
    22921 gtgaactggc agcgttctct cagtttgcat ccgaccttga cgatgcaact cgtaagcagc
    22981 ttgaccacgg tcaaaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg
    23041 ttgcgcagca gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac
    23101 tgtcgaaaat cggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc
    23161 cgttgatgca agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga
    23221 aaggcatcct cgattccttc aaagcaaccc aatcctggta acgtctggcg gcttgcctta
    23281 gggcaggccg caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa
    23341 gatcgcaagc gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc
    23401 caaaatgcgt aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg
    23461 caaagtgatt ggtcaccttg cacacggtaa tctggaatac aagcaccctt acctggaaga
    23521 ccgcgacgtt aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg
    23581 tttgaacatt aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg
    23641 cgttcaatgc gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg
    23701 cggcaatgtt gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat
    23761 cggtccggta aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat
    23821 tgtcagcaac aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc
    23881 gttaccggca tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc
    23941 cgatccgaag gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca
    24001 gggcgtggtt gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc
    24061 gaccgacaat ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca
    24121 ggccagcatt actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg
    24181 ttatttcgta gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta
    24241 gttgacgtcg aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa
    24301 aatggtaatg agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt
    24361 accatcgcaa tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa
    24421 cacccgatcg aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt
    24481 gaaccggtcg acatgaaagg cgagatcggt gaagaagagc gttgggctat tcaccgcgca
    24541 gcaccttcct acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt
    24601 atcgacctga tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt
    24661 gtaggtaaaa ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt
    24721 tactctgtgt ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa
    24781 atgaccgact ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg
    24841 ccgggaaacc gtctgcgcgt agctctgacc ggtctgacca tggctgagaa attccgtgac
    24901 gaaggtcgtg acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg
    24961 gaagtatccg cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg
    25021 gaagagatgg gcgttctgca ggaacgtatc acctccacca aaaccggttc tatcacctcc
    25081 gtacaggcag tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt
    25141 gcgcaccttg acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg
    25201 gccgttgacc cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa
    25261 cactacgaca ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac
    25321 atcatcgcca tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt
    25381 gctcgtaaga tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt
    25441 tctccgggta aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa
    25501 ggcgaatacg atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct
    25561 gtggaaaaag ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc
    25621 acctggacgt cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg
    25681 taacgggtag cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca
    25741 ttaagcctgg tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt
    25801 ctggcggcat tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc
    25861 gcggccagga tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca
    25921 ttagcagctc tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga
    25981 tcgcgcagct gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc
    26041 acaaaagcca gtctggaaac aggctggctt tttttgcgcg tgtgacccgt cctgaatagc
    26101 gttcacatag atcctgctga tataaacccc cctgttttcc cgtttattca ttgatcgaaa
    26161 taagagcaaa aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagac
    26221 aaaaggcgtg acgatggtcg aaaatggcac tttcgtcacc ggggataatc cgttattgaa
    26281 caatttatcc tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt
    26341 ttaaattcgt tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt
    26401 gatccttgcc gcaggcaaag gcacgcgcat gtattctgat cttccgaaag tgctgcatac
    26461 ccttgccggg aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcggc
    26521 gcacgttcac ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga
    26581 caacctgaac tgggtgcttc aggcagagca gctgggtact ggtcatgcaa tgcagcaggc
    26641 cgcacctttc tttgccgatg atgaagacat tttaatgctt tacggcgacg tgccgctgat
    26701 ctctgtggaa acactccagc gcctgcgtga tgctaaaccg cagggtggca ttggtttgct
    26761 gacggtgaaa ctggatgatc cgaccggtta tggacgtatc acccgtgaaa acggcaaagt
    26821 taccggcatt gttgagcaca aagatgccac tgacgagcag cgtcagattc aggagatcaa
    26881 cactggcatt ctgatcgcta acggcgcaga tatgaaacgc tggctggcga agctgaccaa
    26941 caataatgct cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg
    27001 acgtgaaatc gttgctgttc atccacaacg tttaagcgaa gttgaaggcg tgaataaccg
    27061 cctgcaactc tctcgtctgg agcgcgttta tcagtccgaa caggctgaaa aactgctgtt
    27121 agaaggcgtt atgctgcgcg atccggcgcg ttttgatctg cgtggtacgc taactcacgg
    27181 gcgcgatgtt gaaattgata ctaacgttat catcgagggc aacgttactc tcggtcatcg
    27241 cgtgaaaatc ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat
    27301 tagcccatat accgtcgtgg aagacgcgaa tctggcggcg gcctgtacca ttggcccgtt
    27361 tgcccgtttg cgtcctggtg ctgagttgtt ggaaggtgca cacgtcggta acttcgttga
    27421 gatgaaaaaa gcgcgtctgg gtaaaggctc gaaagccggt catctgactt acctgggcga
    27481 tgcggaaatt ggcgataacg ttaacatcgg cgcgggaact attacctgca attacgatgg
    27541 tgcgaataaa tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagtt
    27601 ggtggccccg gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg
    27661 taatgtcggt gaaaatgccc tggtgatcag ccgtgtgccg cagactcaaa aagaaggctg
    27721 gcgtcgtccg gtcaagaaaa agtaattctg gccgggtaat ccggtcatat gggatgagga
    27781 gataacataa tctcccgctc acaagcagta actataaaaa taaccccact ctctacaagg
    27841 ctcggggcgc ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca
    27901 aaaactatgt gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt
    27961 gaaggtttac gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat
    28021 gcagaaggtc atatgacccg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca
    28081 gcggaagaac atcctctgca tggcggtacc ggtattgctc acactcgctg ggcgactcat
    28141 ggtgaacctt cagaagtgaa tgcgcatccg catgtttctg aacacattgt ggtggtgcat
    28201 aacggcatca tcgaaaacca tgaaccgctg cgtgaagagt tgaaagcgcg tggctatacc
    28261 ttcgtttctg aaactgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa
    28321 ggcgggactc tgcgtgaggc tgttctgcgt gctatcccgc agctgcgtgg tgcgtacggt
    28381 acagtgatca tggactcccg tcacccggat actctgctgg cggcacgttc tggtagcccg
    28441 ctggtgattg gtctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgctg
    28501 ccggtgaccc gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt
    28561 tcggtaaaca tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat
    28621 ctgcaatatg acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac
    28681 gaacagccga acgcgatcaa aaacaccctt accgggcgca tcagccacgg tcaggttgat
    28741 ttaagcgagc tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc
    28801 ctcgcctgtg gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg
    28861 gcaggtattc catgcgacgt cgaaatcgcc tctgaattcc gctatcgcaa atctgccgtg
    28921 cgtcgtaaca gcctgatgat caccttgtca cagtctggcg aaaccgcaga taccctggcg
    28981 ggtctgcgtc tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg
    29041 ggttcttctc tggtgcgcga atccgatctg gcgctgatga ccaatgctgg tacggaaatc
    29101 ggcgtggcgt ccacgaaagc attcaccacc cagttaactg ttctgttgat gctggtggcg
    29161 aagctgtctc gtctgaaagg tctggatgcc tccattgaac atgacatcgt gcatggtctg
    29221 caggcgctgc cgagccgtat tgagcagatg ctgtctcagg acaaacgcat tgaagcactg
    29281 gcagaagatt tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca
    29341 atcgcgctgg aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac
    29401 gctgcgggcg aactgaaaca cggtccgctg gcgctgattg acgctgatat gccggttatc
    29461 gtcgtcgcgc cgaacaacga gttgttggaa aaactgaaat ctaacattga agaagttcgc
    29521 gcgcgtggcg gtcagttgta cgtcttcgcc gatcaggatg ctggttttgt ggggagcgat
    29581 aacatgcaca tcatcgagat gccgcatgtg gaagaggtta ttgcaccgat cttctacacc
    29641 gttccgctgc aactgctggc ttaccacgtc gcgcttatca aaggcactga cgttgaccag
    29701 ccgcgtaacc tggcaaaatc ggttacggtt gagtaataca catgccctgc tttcgcaggg
    29761 catttttcta tcccctcact catcgtctca tcttcagcta ttttttaact gtcacagaaa
    29821 gaaaattttt ctgccaaatt gctgtcattc tcgctgctaa cttattgatt tttagccata
    29881 caaaaagact tttcttctgt gtcataaaac tgtcataaac cttacatata actgtcacct
    29941 gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc tcccaggaga
    30001 cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct tatcgatgag
    30061 cgctttctct gtatttgcag aagcaagcct gacaggtgca ggtgcaacct tccctgcgcc
    30121 ggtgtatgcc aaatgggctg acacttacca gaaagaaacc ggtaataaag ttaactacca
    30181 gggtatcggt tcttccggtg gcgtaaaaca gattatcgcc aataccgttg atttcggtgc
    30241 ctctgatgcg cctctgtctg acgaaaaatt ggctcaggaa ggtctgttcc agttcccgac
    30301 cgtgattggc ggcgtggtgc tggcggttaa cattccaggt ctgaagtctg gtgagctggt
    30361 gctggatggt aaaaccctcg gcgatatcta cctgggcaaa atcaaaaagt gggatgatga
    30421 agccatcgcc aaattgaatc cgggtctgaa actgccttca cagaacattg ctgtagtgcg
    30481 ccgcgcagat ggctccggta cctccttcgt cttcaccagc tacctggcga aagtgaacga
    30541 agagtggaaa aacagcgttg gtactggctc taccgtaaaa tggccgatcg gtctgggcgg
    30601 taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa ttggttacgt
    30661 tgaatatgct tatgcgaagc aaaacaacct ggcgtacacc aaactgatct ccgctgatgg
    30721 taaacctgtt ttgccgactg aagaaaactt cgctaatgca gcaaaaggtg cagactggag
    30781 caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcgtggc ctattacctc
    30841 taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca cagaagtgct
    30901 gaaattcttc gactgggcgt acaaaaccgg ggctaaacag gcgaacgacc tggattacgc
    30961 cagcctgccg gatagcgtag ttgaacaggt tcgcgctgcg tggaagacca atattaaaga
    31021 cagtagcggc aagccgctgt actaataaaa ctccaggccg gataaggcat taacggctta
    31081 tccggcatta caaaatgact ttgtaaacgc gtttaactga agagtaactt atggctgcaa
    31141 ccaagcctgc ttttaaccca ccgggtaaaa agggcgacat aattttcagc gtgctggtaa
    31201 aactggcggc gctgattgtg ctattgatgt tgggtggcat tattgtctct ctgatcatct
    31261 cctcatggcc gagcattcag aaatttggtc tggctttcct gtggaccaaa gagtgggatg
    31321 caccgaatga tatctacggg gcgctggtgc cgatctacgg tacgctggtg acttcgttta
    31381 tcgcgctgct gatcgccgtt ccggtgagtt tcggtatcgc cctgttcctg actgagcttg
    31441 cgcctggctg gctgaaacgc ccgctgggta tcgccattga gctgctggca gccattccaa
    31501 gtatcgttta cggcatgtgg ggcctgttta tctttgcgcc gctgttcgcc gtttacttcc
    31561 aggagccggt cggcaatatc atgtcgaata tcccgattgt cggcgcgctg ttctctggcc
    31621 cggcatttgg tatcggtatc ctcgcggcag gcgtgatcct cgccatcatg attattccgt
    31681 acattgcggc ggtaatgcgc gatgtgttcg aacaaacccc ggtgatgatg aaagagtcgg
    31741 cctacggtat tggctgcacc acctgggagg ttatctggcg tatcgttctt ccgttcacca
    31801 aaaatggtgt tatcggcggc attatgctgg ggctgggccg cgcgcttggt gaaaccatgg
    31861 cggtgacctt tatcatcggt aacacctacc agctcgacag cgcctcgctg tacatgccgg
    31921 gcaacagtat cacctctgcg ctggcgaacg aatttgccga agcggaatcc ggtctgcacg
    31981 ttgccgcact gatggaactg ggcctgatcc tgtttgtgat taccttcatc gtcctcgccg
    32041 catcgaagtt tatgattatg cgcctggcta agaatgaggg ggcacgctaa tggctatggt
    32101 tgaaatgcaa accactgcgg cgctggcgga atcccgccgc aaaatgcagg cgcgtcgccg
    32161 cctcaagaac cgtattgcac tgacgctctc aatggcgacg atggccttcg gtctgttctg
    32221 gctgatctgg attttaatgt ccaccatcac tcgcggtatc gatggtatgt cgctggcgct
    32281 gtttactgaa atgacgccgc cgcccaatac ggaaggcggt ggtctggcga acgccctggc
    32341 aggtagcggg ctgttaattt tgtgggccac ggtattcggt acgccgctgg gcattatggc
    32401 ggggatttat ctggctgaat atggtcgtaa atcctggctc gcagaagtta ttcgcttcat
    32461 taacgacatt ctgctctccg cgccgtcgat tgtggttggt ctgtttgttt acaccattgt
    32521 ggtggcgcag atggagcact tctccggttg ggcgggtgtg attgccctgg cgctgttgca
    32581 ggtaccgatt gttatccgca ccaccgagaa catgctgaaa ctggtgccgg acagcctgcg
    32641 tgaagcggct tatgcgctgg gtacgccgaa gtggaagatg atctccgcga ttacgctgaa
    32701 agcgtcggtg tccggaatta tgaccggtat cctgctggcg attgcccgta ttgccggtga
    32761 aaccgcgccg ctgctgttta ccgcgctctc caaccagttc tggagcacgg acatgatgca
    32821 gccgatcgcc aacctgccgg tgacgatctt taagtttgcg atgagcccat ttgcagaatg
    32881 gcagcaactg gcctgggccg gggtgttgat tattaccctg tgcgtcctgc tgctgaacat
    32941 tctggcgcgt gtggtatttg cgaagaataa acacggttaa cactttgccg gatgcggcgt
    33001 aaacgcctga tccggcctac ggtaagcctg attagcgaag tgcatcaggc acgatgagga
    33061 aaagattgca atgagtatgg ttgaaactgc cccgagtaaa attcaggttc gtaatttgaa
    33121 cttctactac ggcaaattcc atgccctgaa aaacatcaac ctggatatcg ctaaaaacca
    33181 ggttacggcg tttatcgggc cgtccggctg cggtaaatcg acgctgctgc gtaccttcaa
    33241 caaaatgttt gaactgtacc cggagcagcg cgcagaaggt gaaattctgc ttgatggcga
    33301 caacatcctg accaactctc aggatatcgc cctgctgcgt gcgaaagtgg gcatggtgtt
    33361 ccagaaaccg acgccgttcc cgatgtccat ctacgacaac atcgcctttg gcgttcgtct
    33421 gtttgagaag ctctcccgtg ccgacatgga cgagcgcgtg cagtgggcat tgaccaaagc
    33481 cgcattgtgg aacgaaacca aagataaatt gcatcagagc ggttactctc tctctggcgg
    33541 tcagcaacag cgtctgtgta ttgcgcgtgg tatcgccatt cgcccggaag tgctgctgct
    33601 cgacgaaccg tgttcggcgc tcgaccctat ctctaccggg cgtattgaag agctgatcac
    33661 cgaactgaag caggattaca ccgtggtgat cgtcacccac aacatgcagc aggctgcgcg
    33721 ttgttccgac cacacggcgt ttatgtacct gggcgaattg attgagttca gcaacacgga
    33781 cgatctgttc accaagccag cgaagaaaca aacagaagac tacatcaccg gtcgttacgg
    33841 ttgattcagg agtgcgttat ggacagtctc aatcttaata aacatatttc cggccagttc
    33901 aacgccgaac tggaaagtat ccgcacgcag gtgatgacca tgggcggcat ggtggagcag
    33961 cagctttctg atgcaatcac cgcgatgcac aaccaggaca gcgatctggc gaagcgcgtc
    34021 atcgaaggcg acaagaacgt caacatgatg gaagtggcga tcgatgaagc ctgcgtgcgc
    34081 attattgcca aacgccagcc gacggcgagc gacctgcgac tggttatggt gatcagtaaa
    34141 accattgccg agctggagcg tattggtgac gtggcggaca aaatctgccg tactgcgctg
    34201 gagaaattct cccagcagca tcagccgttg ctggtaagtc tggagtcgct gggccgtcat
    34261 accatccaga tgctgcacga cgtgctggac gcgttcgcgc ggatggacat tgacgaagcg
    34321 gtacgtattt atcgtgaaga taaaaaagtc gatcaggagt atgaaggtat tgttcgtcaa
    34381 ctgatgacct acatgatgga agattcgcgt accattccga gcgtacttac cgcgctattc
    34441 tgtgcgcgtt ctatcgaacg tattggcgac cgctgccaga atatttgtga gtttatcttc
    34501 tactacgtga aggggcagga tttccgtcat gtaggcggtg atgagctgga taaactgctg
    34561 gcgggaaaag acccgaaaga gtagtgctaa ctgtaggcca gataaagctt tttgccgcat
    34621 ctggcaatca gcccggtcat acatagttac cgggctgata aactttctca ctgaaatatt
    34681 gccacacaaa ctcacctcga aactctcccc agtacatt
//
LOCUS       DAPGRN010000044        34651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPGRN010000044 DAPGRN010000000
VERSION     DAPGRN010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.044"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            105..719
                     /gene="evgA"
                     /locus_tag="RHW33_002268"
     CDS             105..719
                     /gene="evgA"
                     /locus_tag="RHW33_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV1645766.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            724..4317
                     /gene="evgS"
                     /locus_tag="RHW33_002269"
     CDS             724..4317
                     /gene="evgS"
                     /locus_tag="RHW33_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV1645767.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPEHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTALV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIVPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQNN"
     gene            complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="RHW33_002270"
     CDS             complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="RHW33_002270"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV1645768.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="RHW33_002271"
     CDS             complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="RHW33_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV1645769.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="RHW33_002272"
     CDS             complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="RHW33_002272"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV1645770.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8354..9604)
                     /gene="frc"
                     /locus_tag="RHW33_002273"
     CDS             complement(8354..9604)
                     /gene="frc"
                     /locus_tag="RHW33_002273"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV1645771.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPDWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10116..10748)
                     /locus_tag="RHW33_002274"
     CDS             complement(10116..10748)
                     /locus_tag="RHW33_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV1645772.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11044..11319
                     /locus_tag="RHW33_002275"
     CDS             11044..11319
                     /locus_tag="RHW33_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645773.1"
                     /translation="MKVNLMLFSLFLLVSIMACNVFAFSISGSGSEASYKETEKTSAM
                     TTTHSTKFQPSQAILFKMREDAPPLNLTEETTPTYPTKASYLIHPVR"
     gene            complement(11396..11638)
                     /locus_tag="RHW33_002276"
     CDS             complement(11396..11638)
                     /locus_tag="RHW33_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV1645774.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11991..12911
                     /gene="lpxP"
                     /locus_tag="RHW33_002277"
     CDS             11991..12911
                     /gene="lpxP"
                     /locus_tag="RHW33_002277"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV1645775.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13267..13338
                     /gene="ypdK"
                     /locus_tag="RHW33_002278"
     CDS             13267..13338
                     /gene="ypdK"
                     /locus_tag="RHW33_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV1645776.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13403..14641)
                     /gene="alaC"
                     /locus_tag="RHW33_002279"
     CDS             complement(13403..14641)
                     /gene="alaC"
                     /locus_tag="RHW33_002279"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV1645777.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15018..16715
                     /gene="ypdA"
                     /locus_tag="RHW33_002280"
     CDS             15018..16715
                     /gene="ypdA"
                     /locus_tag="RHW33_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV1645778.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            16730..17464
                     /gene="ypdB"
                     /locus_tag="RHW33_002281"
     CDS             16730..17464
                     /gene="ypdB"
                     /locus_tag="RHW33_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV1645779.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            17477..18334
                     /locus_tag="RHW33_002282"
     CDS             17477..18334
                     /locus_tag="RHW33_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1645780.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVCFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRLVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18337..20832)
                     /gene="ptsP"
                     /locus_tag="RHW33_002283"
     CDS             complement(18337..20832)
                     /gene="ptsP"
                     /locus_tag="RHW33_002283"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV1645781.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASTSDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIILRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQAFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(20857..21894)
                     /gene="ypdE"
                     /locus_tag="RHW33_002284"
     CDS             complement(20857..21894)
                     /gene="ypdE"
                     /locus_tag="RHW33_002284"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1645782.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTTWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(21894..22979)
                     /gene="ypdF"
                     /locus_tag="RHW33_002285"
     CDS             complement(21894..22979)
                     /gene="ypdF"
                     /locus_tag="RHW33_002285"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1645783.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVLISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDATHTLATIVNEIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKTSFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(22994..24241)
                     /locus_tag="RHW33_002286"
     CDS             complement(22994..24241)
                     /locus_tag="RHW33_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV1645784.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(24263..24589)
                     /locus_tag="RHW33_002287"
     CDS             complement(24263..24589)
                     /locus_tag="RHW33_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HDV1645785.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(24808..25773)
                     /gene="glk"
                     /locus_tag="RHW33_002288"
     CDS             complement(24808..25773)
                     /gene="glk"
                     /locus_tag="RHW33_002288"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV1645786.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPIEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            25977..27233
                     /locus_tag="RHW33_002289"
     CDS             25977..27233
                     /locus_tag="RHW33_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV1645787.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            27348..27674
                     /locus_tag="RHW33_002290"
     CDS             27348..27674
                     /locus_tag="RHW33_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV1645788.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(27815..29053)
                     /locus_tag="RHW33_002291"
     CDS             complement(27815..29053)
                     /locus_tag="RHW33_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV1645789.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            29389..30591
                     /gene="nupC"
                     /locus_tag="RHW33_002292"
     CDS             29389..30591
                     /gene="nupC"
                     /locus_tag="RHW33_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV1645790.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(30641..32830)
                     /locus_tag="RHW33_002293"
     CDS             complement(30641..32830)
                     /locus_tag="RHW33_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV1645791.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(33038..33113)
                     /locus_tag="RHW33_002294"
     tRNA            complement(33038..33113)
                     /locus_tag="RHW33_002294"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33078..33080),aa:Ala,seq:ggc)
     gene            complement(33153..33228)
                     /locus_tag="RHW33_002295"
     tRNA            complement(33153..33228)
                     /locus_tag="RHW33_002295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33193..33195),aa:Ala,seq:ggc)
     gene            33449..33808
                     /locus_tag="RHW33_002296"
     CDS             33449..33808
                     /locus_tag="RHW33_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV1645792.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            33810..34202
                     /locus_tag="RHW33_002297"
     CDS             33810..34202
                     /locus_tag="RHW33_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV1645793.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(34242..>34651)
                     /locus_tag="RHW33_002298"
     CDS             complement(34242..>34651)
                     /locus_tag="RHW33_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1645794.1"
                     /translation="DLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQL
                     AYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARN
                     ILAAGHAVLACGGMVQSGRSLKQEPTEMIQATA"
BASE COUNT         9089 a         8748 c         8095 g         8719 t
ORIGIN      
        1 aggaagctca aattttctta tttttattga gaaaatgaga tgatgcctta tgtctgtatt
       61 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
      121 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
      181 aaatcttagc agagttgact gaaggcggaa gtgccgttca gcgggtggaa acacttaagc
      241 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
      301 cgctgaggaa gcgccaatat agcggaatta ttattatcgt ctccgctaaa aatgaccatt
      361 tttacgggaa acattgtgct gatgctggcg ctaatggttt cgtgagtaaa aaagaaggca
      421 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
      481 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac tccttatcga
      541 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgctg
      601 aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg atggaaaaat
      661 tagaatgtaa atcactgatg gatctttaca cattcgcaca acgtaacaaa atcggctaac
      721 cacatgaagt ttttacccta tatttttctt ctctgttgtg gtctttggtc gaccataagt
      781 ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
      841 gatccactac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa aaatcttgtg
      901 attgcagtac acaagtccca aacggctacg ttgttgcata ccgattcgca gcaacgggtt
      961 cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat caaattaaca
     1021 ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg tgaagtcgat
     1081 atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc tgcaaccaaa
     1141 ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat gcgaccgctt
     1201 acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga cgaggtaatt
     1261 catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     1321 tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac cagcagtatg
     1381 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc accgcgtcaa
     1441 tataacttct tcttaaccag aaaagaatct gtcattctta atgaagtact caatcgattt
     1501 gttgatgctt taacaaatga agttcgctat gaagtatcac aaaattggct tgatacagga
     1561 aacctggcct ttctgaacaa gccattagaa ctcactgaac atgaaaaaca gtggattaag
     1621 cagcatcccg atttaaaggt gctggaaaat ccttactcgc ccccctattc tatgacggat
     1681 gaaaatggct cggttcgggg cgttatgggg gacattctta atattattac cttgcaaaca
     1741 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgctggaac acagctgaat
     1801 cccggcggat gggatatatt acccggtgct atttatagtg aagatagaga aaataatgtt
     1861 ttatttgctg aagccttcat aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac
     1921 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagcttcat
     1981 tcgcaattaa aagagatgta tccggaggta gagtggataa aagtcgataa cgccagcgct
     2041 gcatttcaca aggtcaagga aggcgaactt gatgctctgg tcgcgacaca gttaaattca
     2101 cgttacatga tcgaccatta ctatcctaat gaactttatc attttcttat tcccggcgtt
     2161 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     2221 aataaagcac tgaatgcaat ccccccaagc gaagttctgc gcctgaccga aaaatggatt
     2281 aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt ttatattgtt
     2341 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     2401 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccggaaa
     2461 gcgctctcgg actccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     2521 agtcacaata gtgcatttga acattatttc actgctgatt actacaaaaa tgcaatgttg
     2581 ccattagaaa acagtgaatc accctttaaa gatgtttttt ctaatacgca tgaagtcaca
     2641 gcagagacga aagaaaaccg aacaatatac acacaggtat ttgaaattga taatggcatc
     2701 gagaaaagat gcattaatca ctggcataca ttatgtaatc tgccagccag cgaacatgct
     2761 gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca tgcactcgaa
     2821 gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct ggcaacaatg
     2881 agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact tctgtcgggt
     2941 tctggtctta gcaaggaaca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     3001 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     3061 caacttcaac cacaatgggt cgatattcct actttagtcc agaacacttg tcactctttc
     3121 ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacgtt tcctgaacat
     3181 tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt actgagtaat
     3241 gctctcaaat ttaccaccga gggggcagta aaaattacga ccgccctggt tcacattgat
     3301 gacaaccacg ctgtaatcaa aatgacgatt atggattctg gaagtggatt atcacaggaa
     3361 gaacaacaac aactgtttaa acgttatagc caaacaagtg caggtcgtca gcaaacaggt
     3421 tctggtttag gcttaatgat ctgcaaagaa ttaattaaaa acatgcaggg tgatttgtca
     3481 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattacc
     3541 caacaagtgg cggctgtcga ggcaaaagca gaacaaccta tcacactacc tgaaaagttg
     3601 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     3661 ctattaggat atgatgttga cgaagccact gatggtgtgc aagcgctaca caaagtcagt
     3721 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     3781 ttgactcgca aactccgtga gcaaaattcg tccttaccca tctgggggct tacagccaac
     3841 gcacaggcta acgaacgtga aaaagggtta aattgcggca tgaacttatg tttgttcaaa
     3901 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     3961 gtacctcagt atcgccacct tgatatcgag gccctgaaaa ataatacggc gaacgatcta
     4021 caactgatgc aggagattct catgactttc cagcatgaaa cacataaaga tttacccgct
     4081 gcgtttcatg cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     4141 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
     4201 ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa
     4261 gaacacattg cagagctgga ccaggagatt gctgttttct gtcaacaaaa taactaaata
     4321 gtggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg
     4381 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     4441 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cctccggctt
     4501 caatcaacat atttctcgcc tgagtttgtg gcaaattaat ggcctcagcc acacttaata
     4561 acggcgctac gggcacaccg acttcatgta ttttggctaa ccaaacttca gctgcctgcg
     4621 tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     4681 tgctgctaaa tctgggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
     4741 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta tcgaaaacat
     4801 caaaaggtgc catgtaggga tggcgatttc ccaggcgttg tggtgacttc cctgtcgcaa
     4861 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     4921 catgcgcccc tctttggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
     4981 aataaacacc gccgcataga tccgcaagag aggtaccaac acgcactggc ggagcatcag
     5041 ggtaccctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
     5101 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
     5161 tgatttcttg taacctttcc catgaaaacc ccagtttttc cattgtacct gggcgaaaat
     5221 tctcagctaa tacatcagct tgtttaagca tatttataaa tatactttta tcgtgatcat
     5281 tctttaaatc aagaaccaca ctctctttac catgattaat aaaactgtaa tagagtgact
     5341 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacctggt ggctcaactt
     5401 taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca ttaaggacat
     5461 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
     5521 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
     5581 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgaca
     5641 aaacccagta cgctcacagc caatgacgct gtaccagtgc gggtgtagac attaaaccgg
     5701 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     5761 agatgttcgc tgttcaaatg acatgccata ccaacaagaa gcagcgccag tggcatcaga
     5821 atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     5881 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     5941 gttgggtccc atgctgccgg aatttttacc ccaaccagca ccaggatcgt tgccagaaca
     6001 ggtgcccata ctacaggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     6061 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     6121 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
     6181 ggatcgagaa ctgcaaatcc caggaatcca atggtaggtg aacctgcaat taatgcacat
     6241 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
     6301 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     6361 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
     6421 agtttattaa atgcccgggc ttgatcttct gaaaaagttt ctcgtctgcc gctaaaataa
     6481 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     6541 gtatttccga taagggtccc ctcaccttcg cataatgagt tggcaagagg agacgatgtc
     6601 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     6661 cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg aataccggtg
     6721 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     6781 tccattaatt tgtcatacct tgcatggtgc aacagatctg ttggtgatgg tgcaccagcg
     6841 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     6901 accggcaggt tatatcgaca aatcgtttca atctccatcc cactgaaacc aaaagcacta
     6961 tcaccttcaa tggcgacaac cggagaacca gaggtcacgc tagcaccgat ggcatagccc
     7021 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
     7081 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
     7141 tggttctcac gcaatacatc gcgcacagca cttaatgcat taaagtaatt taatggttgg
     7201 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
     7261 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
     7321 tgcatactgg atgcaatatc gccaacgact ggcacagcaa tggggcggtt gctgtcaatt
     7381 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
     7441 tgtgccagta accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
     7501 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcgaggat ccctttcgcc
     7561 atagacattg gcaggaatgg aatctgggca ctttcaataa attcacgaag ctgttcgtca
     7621 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
     7681 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga cggattttca
     7741 acttttacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
     7801 ggcaaatcaa gataaactcc gccagggcga cccgatactg agacccggat agcgcgtgcc
     7861 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggcttt
     7921 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
     7981 tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg
     8041 gtcaaaccat tgaggaatcc tggcgcagac actgtcaggc agatccctgg tttttgggta
     8101 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
     8161 cgaatgcctt ccgcctgggc atggcgcgcc atatccgtca caggaatacc tacaacacca
     8221 taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
     8281 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
     8341 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
     8401 aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca gctttaatat
     8461 ctggcgtaaa ggcagagaat ttcattggac aaccaactgt cagatatttt ccacgcaacg
     8521 gttgttccac ttcgacaaca ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt
     8581 tcatacttaa aaccggtgca caaggaatat caaattgagt caaataggcc accgcttcat
     8641 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
     8701 gtcgggcatg ggctgtactg tatgccggat cggtaatcca atctggtttg ccgatggctt
     8761 tgcaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
     8821 tttcccaacc tttacatttc aggatccagc caggctgacc gccaccaccc gcattacctc
     8881 cgcggggaac tgcatcacca aatgtgccat tcggatactg cgggtattct tccagataac
     8941 ccaatttatc gagacgctgc tggtcgcgta atttcacgcg gcaaaggttc aatacggcat
     9001 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
     9061 caagtaaacc gatcagtaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
     9121 ccagcggcgg accgtcccaa aaacccgtag tggatgccgc gccacccgct gcctgagcaa
     9181 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
     9241 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaaaccc atgtgatcaa
     9301 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
     9361 cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta ctgttaagca
     9421 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc gttacgtcac
     9481 caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc atttgagtac
     9541 aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga agtggagttg
     9601 acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat ttaaggcata
     9661 acgcctctat aaataatccg caaaatatcg tcgtagctgc actcgatatt tttacattcc
     9721 tgtatcaagt gctgaaaatc atttcatcgc tacttccaat tcctttctgc atggtaagta
     9781 gtgaggacga aaagaagaat atgataaata ttggttcgtg gtaatcagag agagtcgcga
     9841 catttgtgat gttattctta caactgtaag aagcgccggt gatttacaag taagggtatg
     9901 attacgattt ttaaagctta ccataaaata taacatatta aatgatttgt gatgacacgg
     9961 gattattgcg gtttactttg aggaatataa aatgcgagtt tatagaacag cgagcgccaa
    10021 ataattttat gaccacaacc tgtcgtattg tggtcataaa aaattgtcgg ctggagggag
    10081 ttaaatcctt tacctcaata tactgccagt cagcattagt tcacaaacag tgcttcgcta
    10141 tcgacgatga ttccatcttc cgcaccttta agcgccaggt ttgcctcgta gtattgcttt
    10201 ttatcaagca gtttctgcgc atcagcaagt gcattacgag tctgtttaag cggcatcaga
    10261 tattggtttt ccattacacc aacaccggcc aggcgaagct cttccattgc gcccttttta
    10321 tcccctttcg ccattttttc attggcaatt tttatcgcgg cttctttttc gggtgtcgct
    10381 acataactct cagaaattcc gacagatgca ttaatgacaa tatattggtc atcattgaga
    10441 ttggtctttt tacctggctt agcgaacttt gcccattcgg tactgtcatc tgacaacaaa
    10501 gcggaagctt cattggtcag ttcttttgcc ttttccggat cgccatgaaa cagagccagg
    10561 cgcgctacct gcacgtcacg catcgcatat aaaccttgtt cagagatacg ctctgctgct
    10621 gccgttttct gggtttgctg aacttgctga gtttgctgtt gtgccgctac cggtgcatta
    10681 tcagcagccc atacagttga agatgccaga atagctgtga ccatcgtagc cataattaaa
    10741 cgtttcatac ttacctcatt ctttaacagt tttagaaaac gccgccatca tattgggtga
    10801 gcatgaaatg agaaatcaga gagcaacggt cattttgaaa gggttatctt acacttgtag
    10861 ttattatcgc aagaagtatt ttaatcttac agttgtagga atctgcttcc tgtcacagag
    10921 aactattctt atataccaca aagagatgtc atttattata tcgatgtaaa tactgattaa
    10981 ttatatgtaa atacagccat aacgtcgtag gttttcagtg aaccattttc aggataactt
    11041 tctatgaaag taaacttaat gctttttagc ttatttttat tggtctctat tatggcatgc
    11101 aatgtttttg cattttctat ttctggtagt ggtagtgagg cgagctataa agagacggaa
    11161 aaaacgtcag cgatgacgac aactcactct acaaaatttc agccatcaca ggcgattttg
    11221 tttaagatga gagaagatgc gccaccatta aacctcacag aagaaaccac gcccacttat
    11281 ccgacaaagg cgagttatct tattcatcct gtgcgatagc gtctgtcaca tggcaggtga
    11341 gcaatcatgt ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactacc
    11401 gcctgccata ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa
    11461 atatccccgt caccagttca ttaccgcctg ttaaccacat ggtgaagtaa taaatgagtg
    11521 cagcgaggat caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac
    11581 cgatgtagcc gcttacgcag acaatacaca gggcgagaaa catccataaa taaatcatag
    11641 ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc
    11701 cttaacctct ggcaattttt gctaagcatc aatgaaaaca gcatgttaaa tgcaacacta
    11761 ttgcatacgg aaaatttttt actttgcacg attaataatc agtagctgaa agcagtcagc
    11821 cagtgataaa gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt
    11881 acactcttta caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt
    11941 cgacatggaa tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac
    12001 aatgcaaatt ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttgggcttg
    12061 gtgtactctg gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta
    12121 ttggcgcaat ggcgcgacca ttcctgaaac ggcgtgaatc tatcgcccgt aaaaacctgg
    12181 aactttgctt cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc
    12241 gttcactcgg catggcgctg gtagaaaccg gcatggcgtg gttctggccc gacagtcgcg
    12301 tacgtaaatg gtttgatgtt gaagggctgg ataaccttaa acgcgcacaa atgcaaaatc
    12361 gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga actgggcggt cgcgtgatgg
    12421 gactttgtca gccaatgatg gctacctatc gtccacataa taatcagctg atggaatggg
    12481 tgcagacccg tggtcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg
    12541 gcattgtcag tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg
    12601 gtcgtaaagg cagctccttc gcgccgttct ttgcggtaga aaatgtcgcc acaaccaatg
    12661 gcacctatgt tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa
    12721 aagcggatta cagcggatat cgtttgttca tcaccccaga gatggaaggc tacccgacag
    12781 atgaaaatca agccgctgcc tatatgaaca agattatcga gaaagagatc atgcgcgcac
    12841 cggagcagta cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt
    12901 tatacattta acaaactgct ctgactcttt acctcctcac ataagccata ataacctcat
    12961 ggtttttatg gcttttttct taaggttttt tccttcgctg aatctctaat tattaccgtc
    13021 agaaattaaa ctgtcgccga tatgagacac ctgtaagtta ctaaaagtaa ataagaaaat
    13081 gcctcttgtc tcgtttcagt tttaggcgta cattagcgcc gtctcgagca gtttagctca
    13141 gaattctggg gtgatgccca cggtaaaata caggactttt aaggtcgttt tgcccggtgt
    13201 caactcactg tatcaggtgt aatgaagtca ttcaggcgta acagcaatta cgcggagaga
    13261 tgtaaagtga aatatttctt tatgggcatt tcttttatgg tcatcgtttg ggccggtact
    13321 tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa caggagccat caggctcctg
    13381 tttgcatttc tgaggtcggt gtttattccg cgttttcgtg aatatgtttg ctgctggcgg
    13441 gtagtaaacc gtcggcacgg aacatcgctt taatcccacg aatcgcctga cgaatacggt
    13501 cgcggttttc aatcagtgca aagcgcacat gcgtatcacc gtagtcgcca aagccaatcc
    13561 ccggagagac acagaccttc gcttcgttaa gcaatttctt ggcaaattcc agcgagccca
    13621 tggcagcata cggttccggt attttcgccc agacatacat cgaagccttc ggcatttcga
    13681 ccatccagcc cgcttcatgc agccctttaa ccaatacatc acggcggcgt ttgtactgtt
    13741 cggcaatgtc gcgcacgcac tgttgatcgc cctcgagtgc cgcaatcgcc gccacctgca
    13801 acggcgtaaa ggtgccgtaa tcgtgatagc ttttaatacg tgccagggcg ctgaccagcg
    13861 ttttgttgcc aaccataaag ccgatacgcc agcccgccat gttgtagctt ttcgacagcg
    13921 taaagaactc gaccgccaca tcgcgtgcac ctggcacctg catgattgac ggcgctttcc
    13981 agccatcgta gacgatgtcc gcataggcca ggtcatggac caccagcaca tcgtagcgtt
    14041 tcgccagcgc cacaaccttt tcgaagaact ccagttccac gcattgcgcg gttgggttcg
    14101 acgggaagcc gaggatcatc atcttcggtt tcggataact ttcgcgaatg gcgcgctcca
    14161 gttcattgaa gaaatcgaca ccttccacca gcggcactga gcgtacctga gccccggcaa
    14221 tcaccgcgcc gtaaatatga atggggtaac ttggattcgg caccagcact gtgtcaccgt
    14281 gatccagtgt cgccagcatc agatgcgcca ggccctcttt cgaaccaata gtgacgatgg
    14341 cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc
    14401 gacgtagccg cggaataccg cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca
    14461 cagtacataa tttttcgacg atatgcggcg gagtcgcacc gtccgggtta cccatactga
    14521 aatcgataat atcttcgccg cgccgacgcg cagccatttt cagttcagcg gtaatgttaa
    14581 aaacataggg cgggagacga tcaatgcgcg taaagcgacg ttcagggcga gtgtcagcca
    14641 taatttcctc agattaacgt tagcgcccgg accgtccgag cgacgctgcc acgatggtgg
    14701 ctgttttgaa aatagcctga ttaatttctg tctgtccagc gttttagaac atttttatta
    14761 atgaccttaa atgaggaact ctgctttaaa aacaggaaat ttatctgcgc taacaagaaa
    14821 attaccactc attttattaa cataataatt tcaataaatt tactaaccag aagtgcatta
    14881 tcaagagatg cattgctaat acaccattta tctttcaatc cccttttaaa attgtggttt
    14941 ttcctcaaaa acggcctgct ggtcatcagc cttcaggtta cctatcatag aggtttaatc
    15001 cttattcaga gtcacccgtg cacgaaatat tcaacatgct gctggcggtc ttcgatcggg
    15061 cagcgttaat gcttatctgc ctgttctttc tcatccgtat ccgcctgttt cgcgaactgt
    15121 tgcacaagtc ggcacactca ccgaaagagt tgctcgccgt taccgccatt ttctcgctgt
    15181 tcgccctgtt cagcacctgg tccggcgttc ccgtagaagg ctcgctggtg aacgtacgta
    15241 ttatcgcggt gatgtccggc gggattctgt ttggcccgtg ggtgggcatc attaccggcg
    15301 tgattgcggg tattcatcgt tatttaattg atatcggcgg cgtgacggcg atcccctgct
    15361 ttatcaccag cattctggct ggttgtatat cgggctggat caacctgaaa atccccaaag
    15421 cacagcgctg gcgcgtcggt attctcggcg gcatgttgtg tgaaacgttg accatgattc
    15481 tggtgattgt ctgggcacca actaccgcgt tggggatcga tatcgtctct aaaatcggga
    15541 ttccaatgat cctcggcagc gtctgtatcg gctttattgt gcttctggtg caaagcgttg
    15601 agggcgaaaa agaggccagc gccgcgcggc aggccaagct ggcgctggat atcgccaaca
    15661 aaacgctacc tctgtttcgc catgtcaata gcgagtcatt acgcaaagtt tgtgaaatta
    15721 ttcgcgatga cattcacgcc gatgcggtgg caattaccaa taccgatcat gtgctggcct
    15781 atgttggcgt gggtgaacat aactatcaga atggcgatga cttcattagc ccgactaccc
    15841 gtcaggcgat gaattacgga aaaatcatca ttaaaaacaa tgatgaagcc caccgcacac
    15901 cagagattca ttccatgctg gtgatcccat tgtgggagaa aggcgttgtg accggaacgc
    15961 tgaaaattta ctactgccac gcgcatcaga taacctcgtc attacaggaa atggcggttg
    16021 gtctgtcgca aatcatctcc acgcaactgg aggtttcacg cgccgagcag ctacgtgaaa
    16081 tggcaaataa ggcagagctt cgcgccctgc aaagcaaaat taatccccat ttcctgttta
    16141 acgctttgaa cgctatttca tcgtcaatcc gtctgaatcc ggatactgct cgccagctga
    16201 tctttaatct gtcgcgttat ctgcgctata acattgaatt aaaagacgat gagcaaatcg
    16261 atatcaaaaa agagctgtat caaattaaag actatattgc cattgagcag gcccgctttg
    16321 gcgacaagct gacggttatc tatgatattg atgaagaggt gaattgctgc attcccagcc
    16381 tgctgatcca gccgttggtg gagaatgcca ttgtccacgg tatccagcct tgcaaaggta
    16441 aaggcgttgt caccatcagc gttgcagagt gcggcaatcg ggtacgcatt gccgtgcggg
    16501 ataccgggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat gaaatgccgg
    16561 gcaataaaat tggcctgctg aatgttcatc atcgcgtgaa gttattgtat ggcgaggggc
    16621 tgcatatccg ccgcctggaa ccaggaacgg aaattgcgtt ttacattcct aaccaacgca
    16681 ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg tgaaagtcat
    16741 cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta aagagcacag
    16801 ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt ttttgcagca
    16861 taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg gtgtgttgct
    16921 ggcacaaaac atcagccagt tcgcccataa accgtttatt gtgtttatca ccgcgtggaa
    16981 agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca aaccgtatca
    17041 ggagtcacgc attaccggga tgctgcaaaa actggaagcg gcctggcagc aacagcagac
    17101 cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc tggtgaaaga
    17161 tgagcgaatc atcgtcacgc caattaacga tatctattac gccgaagcgc acgagaaaat
    17221 gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta ccgaattttg
    17281 cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg tcaatctgaa
    17341 caaaatccgc gagatcgaac cgtggtttaa taacacctac attctgcgac tgaaagatct
    17401 ggattttgaa gtgccggtca gccgcagcaa agttaaagaa ttccgccagt taatgcatct
    17461 ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat ttttcgccga
    17521 tctgttcagc ggcctggtgc ttaacccgca actactgggg cgcgtctgtt ttgccagcca
    17581 gcctgcctcg ttgccggtgg gcagtttatg tattgatttt ccccgtctgg atatcgtgct
    17641 gcgcggcgaa tacggcaatc tgctggaagc aaagcagcaa cgtttggtgg aaggagaaat
    17701 gctgtttatt ccggcgcgcg cggctaattt accggtcaac aacaaaccgg tgatgctgtt
    17761 aagcctggtg ttcgctccga cctggcttgg gttatcgttt tacgatagcc gcaccacgtc
    17821 gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg aaggtgaagc
    17881 gatgcttacc gccctcaccc atcttagccg ttcgccgctg gagcaaaata tcattcagcc
    17941 gctggtgtta agtttgctgc atctttgccg taacgtggtg aatatgccgc cgggcaattc
    18001 gcagccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg ataattatgc
    18061 ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca atcatctgtc
    18121 aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt gggtgcgaat
    18181 ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag tggcacagcg
    18241 ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt ttggtctgac
    18301 gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt ctgtttccag
    18361 cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga agtttttatt
    18421 caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt cgttagcacc
    18481 cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt caaccggttt
    18541 cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg tgtgcgggat
    18601 cgccacgcca aaaccaacgc cagtggtgac aatctcttcc cgctgccaga catcctcttc
    18661 cagctcgaac ggatgttcag tgcgcccgtt aacgccgagg ttaccgcaca ggaactggat
    18721 cgcctgctct ttattgctaa aggcctgatc aacgaagata ttctccagcg ccagcagcgg
    18781 gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct gtgcgctgcg
    18841 acattcacac gcctgacgtg ccagttcccg acacgcctcg ctatccagtt gacgaagctg
    18901 gcttttcacc gccggaatgc gcgggctact catactcagc tcatccaggc ccagcccaag
    18961 cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta cccatttgcc
    19021 tcgctgatgc gcagtggtaa ctatctgctg caacatgcgc aggaacgatg gcgtaatcgg
    19081 gttatacagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat actgggtcat
    19141 atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga tgatgtagca
    19201 caccgacggc acttccacca tgatacccag cgtaatcgtc tctgcatgac gcaggccatc
    19261 gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga tctgatcgag
    19321 gctgtgaacc atcgggatca tcaactgggc gttgccgaaa ctggcggcgc gcaaaatggc
    19381 ccgcagttga gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg cgcgatagcc
    19441 gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat cgcctccgat
    19501 gtccattgtg cgcaagataa tcggcttgtc gcccgctgcc agtagcacct gctggtaggc
    19561 ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg
    19621 gaacagcccg acgccttccg cgccgttggc aaacgcgcca ggcgcttcca gagcggtgcc
    19681 aatattcgcc gcgatatcaa tacgcttgtt atcacgggaa taagccagct gcgcggcagc
    19741 ctgcgcctgt tgtttttggc gtttatccgc cagcgtttgc gcgacctgat aataaccgct
    19801 caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg
    19861 cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga tcgccgaggc
    19921 gcgggccaga atcagggtat gcgaggtgcg cccggttttc tccagaatca tgcccgcaag
    19981 attttttaaa tccaggctta aaaactggct tggggttaaa tcctcggcca ccagaatagt
    20041 cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtaa tatgcagcaa
    20101 ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcgc tggtagaagc
    20161 agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca gcccctgatg
    20221 ctgttctgtc atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa
    20281 atgggcgctg aggatagttt tgctttcgcc gtcacgctca cgcaattgct ggttcagttg
    20341 ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat
    20401 tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc
    20461 gcttgccagc acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag
    20521 cggatgcgcc gtcagttctg cctgcgtagg ctgaacgctg tcgctgtcga taaagcgcac
    20581 ctggatgtac tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag
    20641 gctgcaactg tcattaaata gggtgccggt gccaatcagc gccagcgagc ttttggcatc
    20701 ggcctttgcg ttctggcgat ggttaataaa agtgatttcg ctttgccact ggctgcactg
    20761 ttctttaagt tcccacgccg gacgagcatg tagaccgtta ggcaaaggac agagaaattg
    20821 aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg tcagttgaac
    20881 aaccgtctca cgcgtaagac gttgaataag ggcagataaa agttgctcca tctgcaaaat
    20941 gtcgcggcaa tcggcaatcg acgcggcgca atgtccgtgg cgggtggcag gccccatcac
    21001 cactgtgggt acgccagtac cggttaagtg caccgctcca ccgtccgtgc cgccgttact
    21061 gaacatatcc gcctgtaacg gcacgccaat ttctgccgcc acggtttcga tccaggtggt
    21121 gagttttggc ggcgcaatca gtgacttgtc gcttaacacc agcatcgggc cgttaccaat
    21181 ctggcgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg tatcaaggac
    21241 aatggcgacg tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta atcccacctc
    21301 ttcgctggaa cttgccacca gccacacttc cgcaggcagt tcggcgtcgt gcagctcacg
    21361 tagtaacgtc accagcagat agcaaccgag gcggtcatca aaggctttcc ccatcactcg
    21421 ctggtggggg agaacctgaa aagtggtatc gaacgtgacg cgatcgcctg gacgaattcc
    21481 cgcctgcatc acttcgtcat acgagcgcgc gccaatatcc acgcgcatgg cgctgacgtc
    21541 attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac gggtggtgat
    21601 gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca gcacatcaat
    21661 tgcgccttcg cgggagatac tgcgcaccat aaatcccact tcgtccatat gcgcacagat
    21721 catcaccttc ggacctgtcg attcattcag gcggatcagc accgatccca gaccatcaaa
    21781 tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca cttcctgttc
    21841 cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat ccattatgcc
    21901 tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc ttgcggggtg
    21961 accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat ccccggctcc
    22021 acggtcagta acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa gcgcggatct
    22081 tcatgtactt caatgccgat agcatgaccg gtgttatgac cgaaatagtc gccataacct
    22141 gcttctgtaa tgacccggcg cgcggcgtcg tcaacctgtt ggcaacgcac gccggggcga
    22201 atcgcggaga ttgccgcgag ctgtgcctgc aaaacaatct gatagacgtt aaacagcggg
    22261 tgagattcgg cacttacccc ttcgccattc accagcaagg tgcgcgtcat atcagagcag
    22321 tacccctgat agagcgcacc aaaatcaaga gtgacaaact ctcccgctgc aacaatcttg
    22381 tcgctggctt tgccgtgcgg cagcgcccca cgccagccac tggcgacaat ggtgtcaaaa
    22441 gaggtttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc tatctcgcgc
    22501 tcgctcatcc ccgcctgaat aaagcggcga atatgctctg caccgcgatc ggcaatccca
    22561 caggcaaggc ggattttctc cacctcctct ggcgttttga tttgccgcag cacatccggc
    22621 gtggcgctaa ccagtttcgc attgagttca gactgccagc ggtgcgcggt ttcccaactc
    22681 acctgctggc cttcaaaacc gagcgtttgc aactgttcat cggcaatgat ttcattgacg
    22741 atggttgcaa gcgtgtgtgt cgcgtcaagc aaatgcagct ggtaaccttg cgtacgggct
    22801 tctacatccg cgtaatagcg cgagtccacc agaatgtgcg cactttcacg gctaatcagc
    22861 acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg tgaggagaga
    22921 agcactgcat ccagttgttg cgccttaagc cagtcgcgca gcgaagcgag taatgtcatc
    22981 tggattttcc ttcttacagg ctatcgatta acaatttgcc tttgcggaac atcatcagac
    23041 gcaggaacac caccatcagc gcagtaatga ttgcccccag cgcgatcccc gccatataga
    23101 cgcccaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac cattgcactg
    23161 cgcccagcca gacagcggcg gttgagccga caatagcgcc gaccatatac gacggaatgg
    23221 cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc ataaacgcca
    23281 ggaacatggc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt ttgccggtta
    23341 aacgacggtc gataatggtc gccagcccca gaccaatcgg cggaataacg attgcgatag
    23401 aacgtgcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg aaacctgcgg
    23461 ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc atcgcataca
    23521 ttagcgcgcc cttctcacct gcggcagtca gcacggtacg gataccaccg ttgatccagc
    23581 cgccaaaggg ggtgatcacg tagtacatcg ccagcatgac aaaaatagcg gaaagaatcg
    23641 gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt ttctggttca
    23701 tccatttcac aaggtaacca gcaacgatag atataatcag cgcaccaatg aaggtggacg
    23761 gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc agttgcgtcg
    23821 gctgggtaga catcaagccg ccgataaaac cagccggaaa cgccagtttg ccaccgatag
    23881 agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg ccgccgaagg
    23941 actgcgacaa ccaggcaaat ttcagtaagg agaggtcgaa accggagaat ttcccgctat
    24001 taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa gcaatcagct
    24061 ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt gaaatccctg
    24121 acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc ggattcttaa
    24181 ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt ttaatggcca
    24241 taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct tcgatttctt
    24301 tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa acgtcgcgtg
    24361 attcgaaacg ttcgttatct tccggggtaa ctgccacgga gtggatgatg atggtcgctt
    24421 cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg gtttcaattt
    24481 tcacttcata accggcttct accgccgctt cttccagcgc ctgagcggcc ataaaggtgt
    24541 gagccaggcc catcgggcag gcacataagg caatcagttt cttactcatc gttaattcct
    24601 cgttcagtga tagttgacga gtgaaattat gaacgtgcaa aacaaatcgc cgttaccaga
    24661 caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg caggtcggcc
    24721 ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaaagat tatcgggaga
    24781 gttacctccc gatataacag gaaggattta cagaatgtga cctaaggtct ggcgtaaatg
    24841 tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg gaatatcatg
    24901 gacatattct ttaaagcgcc ctttatcttc aaatgcggca cggaaaccag aggctttgaa
    24961 gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa atgtcccgag
    25021 attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg acaacgcacg
    25081 gcggcaatcg gtgcagctgt cagccagcgc gcgttcggta atatcttttg gcttgagatt
    25141 ttctggcagg cggttgtcgg ctttaacaat tgcgcgatac aaattcacca gccctgggcc
    25201 agaaagcacg cgttccgccg aaacatgacc aatttccgca cgcagtattt cgaggataat
    25261 ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg gcaagcttac
    25321 ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cccgttccgg caccgtaaac
    25381 cgcaataggc ttaccttcta tcggctctgc gccaccaaac tgaatcagat gctctttttt
    25441 cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca gatggctaaa
    25501 accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg tcattgccac
    25561 ccagtctccg gtaatcgggc aagcgatggc aatacagccg tctttcacct cgaccttatg
    25621 ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc ctgaataggt
    25681 cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg cgttggtgcc
    25741 gcccacatca ccgactaatg catactttgt cattcttcaa ctgctccgct aaagtcaaaa
    25801 taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga aaggcaggct
    25861 cccagtaaat atcgatctgg gtcacacaat tactttatcg tttcagcacc aattgcagcg
    25921 atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa tagaacatgc
    25981 tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca attgggattg
    26041 cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag aatttgctct
    26101 ggcaacgact gccgggaact ctgggaatag cacaggattc acctctctgg atcatcggtg
    26161 tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag ggtcatgccg
    26221 gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccggt tccgccctct gcgctaccag
    26281 gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg ccggaacatc
    26341 cgatcatgac cgtcaatatc gcccttgcgg ttgccattgg cgctcgtctg ttaccgcgcg
    26401 tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg ctgtttggta
    26461 cacctgttgc ggcggcgttg atattttcgc aaaccttaaa tggcagtagt gaagttccgc
    26521 tatgggatcg tctttttgcg ccgttaatgg cggcagcagc tggtgcactt actaccggat
    26581 tatttttcca tcctcatttt tcattgccca ttgcgcatta cggacagatg gaaatgaccg
    26641 atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg gtcgccgtat
    26701 ggtgcttacc acggttgcac gcgatgatgc atcaaatgaa aaatccggtg ctcgtgctgg
    26761 gtattggtgg atttattctc ggtattctgg gggttattgg tggaccagtt tcgctgttta
    26821 aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc agcgattact
    26881 ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt ggctttcgcg
    26941 gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg ctgcatgagc
    27001 acgttcccgc cgtaccagcg gcaataaccg tttcctgcgc tattctcggc atcgtgctgg
    27061 tggtaacacg cgatggctgg ttaagtcttt ttatggcggc agtcgttgta cccaatacca
    27121 cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta gcaggtaagc
    27181 cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt tagcaaaaaa
    27241 tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct ccccgtaata
    27301 ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg ttcaggtcac
    27361 tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca ggcgaaatca
    27421 ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt aattactggg
    27481 acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgaatgg cgtaaaaacc
    27541 gctggtggcg tcatgataat ggctaccatc gtggctggga taagcgtaaa gcatatgagc
    27601 gtggctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc cgcggacatg
    27661 gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac gctagcgcgt
    27721 cttatcaggc atacaaaccg gctctttaac gcgtagggcg gataaggcgt ttacgccgca
    27781 tccgccaatg gtgccagatg cgacaccagt tgaactatag ccccagcgcc gtacccacta
    27841 acaaccagat attcagcgct actaccagca ccacaatcac ccagcctgtc tgttttacgc
    27901 gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc agcagtggaa
    27961 ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga atccgtgtcg
    28021 gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta cgacgcaccc
    28081 acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc agtgtcccca
    28141 ccaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt gccgcagcat
    28201 ggcttaacag cggttgcagc gtcaaataag cctcatcaag atcggcaaca ccagtatgac
    28261 cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca aaaccggcaa
    28321 tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga cgcgaaccgc
    28381 catgtaaatg ctgagtgagc gaagagtgca aataaatcac atgcggcata atcgtcgccc
    28441 ctaacacgcc tgctgccagg aagaccgcct ccgaagtagg taaactcggg atcaccattc
    28501 ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg taagccgcgg
    28561 caacaaacaa cagtaacccg ccaatcactt tctccagcgg tttttgcccg cgacgttgca
    28621 gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac aacgaaacac
    28681 caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg gttgccatcg
    28741 caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag tgatcgcgaa
    28801 tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg atctgaatca
    28861 gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg aagctggcac
    28921 ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc gcaatgaacg
    28981 caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta ctctcaacgc
    29041 gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat gctataccaa
    29101 acgagaatga ttatcaaatt catttaaatg ggttgtggtg atttctctga tagaccagga
    29161 ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg ttagcacatt
    29221 tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagct cactattttt
    29281 gaagcttgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct taaccatgtt
    29341 acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat ggaccgcgtc
    29401 cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt aagcagcgac
    29461 cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt gttactggcg
    29521 tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc cgaaatgttc
    29581 gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag catgaatgat
    29641 caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat ctctgcactg
    29701 atcggtattc tccagcacat tcgcgtgttg ccggtgatta tccgcgcaat tggtttcctg
    29761 ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag ctccctgatt
    29821 ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat ctcccgcaat
    29881 cgtatgtaca ccatggcagc aacggcgatg tccaccgtat ctatgtccat cgttggtgca
    29941 tacatgacca tgctggagcc gaaatatgtc gttgcggcgc tggtactgaa catgttcagc
    30001 acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga agaaaacatt
    30061 cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga atacattctg
    30121 gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat cgccctgatc
    30181 gccgcgctga acgcactgtt tgctaccgtg actggctggt ttggctacag catctccttc
    30241 cagggcatcc tgggctacat cttctatccg attgcatggg tgatgggtgt tccttccagt
    30301 gaagcactgc aagtgggcag tatcatggcg accaaactgg tttctaacga gttcgttgcg
    30361 atgatggatc tgcagaaaat tgcctctaca ctctctccgc gtgctgaagg catcatctct
    30421 gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg tgcagttaaa
    30481 ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaagct ggtttacggc
    30541 tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta agaccataca
    30601 taaaaaagcc ggggataatt ccccggtttt tttacgcctg ttaatcagct aatggctgcg
    30661 ggcgaccaat caaatacccc tgcagatatt gcaccccgag cttatgcaat aacgcctgct
    30721 gctgttgcgt ctcgacaaac tccgcgacca cactcaatga cttcgctttc gccagatcgg
    30781 taattgatct cacaatcatc gcatccagcg tattcgtgac aatatctttc acaaagacgc
    30841 catcaatttt gatgatatca gcctgcaaac gctttaaccg ttcgtagttg gcatagccgg
    30901 tgccaaagtc atcaatcgca atccggaaac caaacttatg cagctgctcg atgttgtaca
    30961 tgctgctttc tgcgttagaa aacgcctgct cctcggtgat ctcaagaatg accgcctgcg
    31021 gggagatgtg ataacgctta aatagacgaa taatccgccc ggcaatattc ttttgcagca
    31081 gcgtgagcgg cattaaatta actgaaaagc gcgggccttt tttgtcgcaa gggtgtgttg
    31141 ccagccactt caacaaggat tccagcactt gcaaatcaaa acgcgcgcta aggttaaact
    31201 gagcaataag gggcagaaac ttatccgggg tcataatgcc gccgtcatat ttcagtcgcg
    31261 cgaggatctc atcataacct tcaccctctt tgttgcgaat tggctgggcg tagagcagca
    31321 aatcaccctg atctaacgcc gttcgaatgg tattcagcaa tagcacctgt ttagtggtct
    31381 gcccggaaac catctcctct ctgctatcca gcgccagcac atgatgatgt gcacaggatt
    31441 gctccgccag ccagcttaac tgccccaaca agggttgcag ggtttcctga tttccatcaa
    31501 aacgccccca ggcagcacca tagcccatat ccagcccggt attgttccag tgaatttgcc
    31561 gactattcag gatgttaacc atatgctgga gtcgcccttc cgtttccggc ccgctcagca
    31621 ctaacagcag ttcactaccc ggcaattgat acaacttttc gttttcctgc atcaacggca
    31681 gcagcgtacg gtaaattgat cggatacagt gaacgcgcat cattaacccg taatgacgac
    31741 tcataaactc aagattatca atgcgcaggc agcaaaaact cttgcccgcc tcttgctccg
    31801 gcgcttgttc caacgcacga aaattgggta ggagcgttaa cggatcggtc agcgcctgca
    31861 aatgccagcg tcgattaagc cattcactgc gatgataaat gcgcaccata tagagcaggc
    31921 aaacgctaaa ggaaatcagc accgcgagaa taaatgccag cgaatattcg gtttccaccc
    31981 cttgcaaaaa gttctggttg taattcagca ggcaaagcgt tgaaacagcc caagtgagat
    32041 ttaaaaacgg atagcgaagc ttaccgaccc cgagggtaaa gatgatgaag aaaacgggta
    32101 ccaggtaacc ggcaataaag tcgttttcat aaggtgtgca caacagaagt agcagcacgc
    32161 ttaacgctgc cagccagctt aaggtaaatg cgcgtttctc tttgcccaac gacggagcga
    32221 tatccctgcg ccacaatatc tgcgcaaagt ggggacttac aatcatgcga gtgagatagt
    32281 agaaaagcat gttgtaaatc agcacagcgg tgaaaaggct tagcaaatca acgaccgtga
    32341 aaatggcatc cgcatcgccg aaaaaggtgg atatctttag cgggaaatca aagaagctcc
    32401 ccacaagata catgctgcat ttgatgccga tcggcgtcac caaaccaagc cagaacagac
    32461 gttgccagac atatcggctg gtcaatccat aacgccaacg cgtccccagc tgccagcgta
    32521 atattgcgca ggcacacaac acgacaaacg tctggctacc gagcatgacc gcagtctgta
    32581 aaaaagagag atgaaagttc cacagattgg tgcaaaacat cccgcataaa acgggaacga
    32641 caccgcgcca gccaaagata aacaagattg acagcattac gcacagtggc atccacgcca
    32701 gaaagatata actggaatgg ataatggcaa gtggcgaaat aaaacgggaa agctgaataa
    32761 gtaccacggt gagcgtaaac gctagtgtga atatcttgat attttttatc aggttatgct
    32821 ccacaaacat gagtacacta ctctcactga tcggggtttt caggcacaca aataataagt
    32881 ttattcgcat cgcgaagcaa acaggggcca gcctgttgcc gatttttcat ccaatgatgc
    32941 tttcaccagg cggcgctaac ccggacgtag actgtattac aaaagcggca aaaagcagag
    33001 acaaaaaacc cccgctttgc agcgagggtt ggaaatttgg tggagctaag cgggatcgaa
    33061 ccgctgacct cttgcatgcc atgcaagcgc tctcccagct gagctatagc cccacgatgc
    33121 gtttacgtac caaatttgct gggtgcaaaa tttggtggag ctaagcggga tcgaaccgct
    33181 gacctcttgc atgccatgca agcgctctcc cagctgagct atagccccgt cacgtaaagc
    33241 ttgtcgagtt gacgggcggc atcatatgaa ttccgcccga atgtgtcaac ggcaaattgc
    33301 aacgtgtagt ttcaatcgct gaaaaatcag gcaaatgaac aattttggaa aaccgctcgc
    33361 attcagtagt tattcatgtc acggtttcct gtaaagtggt gctataaaat gaactactaa
    33421 tagacccaca tacattcagg gaattgttat gttcaaggag aggatgacgc cagatgaact
    33481 tgccagactg accggctata gccgccagac cattaataaa tgggtacgca aggaaggctg
    33541 gacaacgtca ccaaaacctg gcgtccaggg tggcaaagcc cgactggttc acgtcaatga
    33601 acaggttcgt gaatatattc gtaatgctga acgtccagaa ggtcagggag aagcgcctgc
    33661 cctttctggt gatgcgccgc ttgaagtatt gctggtgaca ctggcgaaag agatgacgcc
    33721 agttgaacaa aaacagttta catccttgct tctgcgggaa gggattatcg gattgttaca
    33781 acgcttaggg attcgcgata gcaaataata tgaaaagatt acgcaataaa atgaccaccg
    33841 aagaactggc tgaatgcctc ggtgtggcta aacaaactgt taaccgttgg atcagagaaa
    33901 aaggctggaa aacggaaaaa tttcccggcg tgaaaggcgg tcgtgccaga cttattctgg
    33961 tcgatacaca agtttgcgag tttattcaga acacgccagc cttccataac acaccgatgc
    34021 ttctggaagc cgaagaaccc cttgccgaat acgcaccggg gattcgcact cctgcttatc
    34081 ggcaaatcat cagcgcaata gacaacatga ctcacagtga gcaggaaaaa gttgcgcaat
    34141 ttttgtcacg cgaaggaatt cgtaatttcc ttgctcgtct cgacatagat gaatcagctt
    34201 gacatcctcc acgccctgaa tgacgaggat ccctgctacg ttcaggctgt cgcctgaatc
    34261 atttcggtgg gttcctgctt caacgagcgg cctgactgca ccatccctcc acaggcaaga
    34321 acggcgtgcc ccgccgctaa aatgttacga gcgccgttta catcggcgtt cgctgtatat
    34381 ccacatacct ggcatctgaa tttactttgt gacaggcgat tttctttcgc tgtatgacca
    34441 cagcacgcgc aacgctggct tgtgtacgct ggcggaacag caagcacctg accgccacgc
    34501 cagagctgct tgtacgcaag ctggcggcgc atttcatacc agccctgatc cagtatcgaa
    34561 cggtttaaac ctgattttgc ccggacattg cgccccggct ggctgaccgt acccgctgct
    34621 gactttgaca tgttgctgac cttcaaatcc t
//
LOCUS       DAPGRN010000045        33155 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPGRN010000045 DAPGRN010000000
VERSION     DAPGRN010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.045"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(177..2444)
                     /gene="adiA"
                     /locus_tag="RHW33_002299"
     CDS             complement(177..2444)
                     /gene="adiA"
                     /locus_tag="RHW33_002299"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV1645795.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2643..3551)
                     /gene="melR"
                     /locus_tag="RHW33_002300"
     CDS             complement(2643..3551)
                     /gene="melR"
                     /locus_tag="RHW33_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV1645796.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSRATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3834..5189
                     /gene="melA"
                     /locus_tag="RHW33_002301"
     CDS             3834..5189
                     /gene="melA"
                     /locus_tag="RHW33_002301"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV1645797.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYDAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5292..6713
                     /gene="melB"
                     /locus_tag="RHW33_002302"
     CDS             5292..6713
                     /gene="melB"
                     /locus_tag="RHW33_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV1645798.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGVVSDVKA"
     gene            complement(6852..7481)
                     /locus_tag="RHW33_002303"
     CDS             complement(6852..7481)
                     /locus_tag="RHW33_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV1645799.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7604..9250)
                     /gene="fumB"
                     /locus_tag="RHW33_002304"
     CDS             complement(7604..9250)
                     /gene="fumB"
                     /locus_tag="RHW33_002304"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1645800.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9328..10668)
                     /gene="dcuB"
                     /locus_tag="RHW33_002305"
     CDS             complement(9328..10668)
                     /gene="dcuB"
                     /locus_tag="RHW33_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV1645801.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11239..11958)
                     /gene="dcuR"
                     /locus_tag="RHW33_002306"
     CDS             complement(11239..11958)
                     /gene="dcuR"
                     /locus_tag="RHW33_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV1645802.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11955..13586)
                     /gene="dcuS"
                     /locus_tag="RHW33_002307"
     CDS             complement(11955..13586)
                     /gene="dcuS"
                     /locus_tag="RHW33_002307"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV1645803.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13767..13997
                     /locus_tag="RHW33_002308"
     CDS             13767..13997
                     /locus_tag="RHW33_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV1645804.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            14009..14281
                     /locus_tag="RHW33_002309"
     CDS             14009..14281
                     /locus_tag="RHW33_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1645805.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14508..14804
                     /gene="ghoS"
                     /locus_tag="RHW33_002310"
     CDS             14508..14804
                     /gene="ghoS"
                     /locus_tag="RHW33_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV1645806.1"
                     /translation="MEGKNKFNTYVVSFDYLSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14832..15005
                     /gene="ghoT"
                     /locus_tag="RHW33_002311"
     CDS             14832..15005
                     /gene="ghoT"
                     /locus_tag="RHW33_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV1645807.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15124..16641)
                     /gene="lysS"
                     /locus_tag="RHW33_002312"
     CDS             complement(15124..16641)
                     /gene="lysS"
                     /locus_tag="RHW33_002312"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV1645808.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16878..18335)
                     /gene="dtpC"
                     /locus_tag="RHW33_002313"
     CDS             complement(16878..18335)
                     /gene="dtpC"
                     /locus_tag="RHW33_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV1645809.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18394..20541)
                     /gene="cadA"
                     /locus_tag="RHW33_002314"
     CDS             complement(18394..20541)
                     /gene="cadA"
                     /locus_tag="RHW33_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV1645810.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20621..21955)
                     /gene="cadB"
                     /locus_tag="RHW33_002315"
     CDS             complement(20621..21955)
                     /gene="cadB"
                     /locus_tag="RHW33_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV1645811.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVI
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22321..23859)
                     /gene="cadC"
                     /locus_tag="RHW33_002316"
     CDS             complement(22321..23859)
                     /gene="cadC"
                     /locus_tag="RHW33_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV1645812.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     INYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24608..25180)
                     /locus_tag="RHW33_002317"
     CDS             complement(24608..25180)
                     /locus_tag="RHW33_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1645813.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     GSAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(25277..25474)
                     /locus_tag="RHW33_002318"
     CDS             complement(25277..25474)
                     /locus_tag="RHW33_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645814.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(25486..25974)
                     /locus_tag="RHW33_002319"
     CDS             complement(25486..25974)
                     /locus_tag="RHW33_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645815.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTRDVGESEWIHFT
                     GTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDANAECLPGLPTF
                     DW"
     gene            complement(25971..26348)
                     /locus_tag="RHW33_002320"
     CDS             complement(25971..26348)
                     /locus_tag="RHW33_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV1645816.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(26438..26806)
                     /locus_tag="RHW33_002321"
     CDS             complement(26438..26806)
                     /locus_tag="RHW33_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV1645817.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(26969..27190)
                     /locus_tag="RHW33_002322"
     CDS             complement(26969..27190)
                     /locus_tag="RHW33_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV1645818.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(27253..27729)
                     /locus_tag="RHW33_002323"
     CDS             complement(27253..27729)
                     /locus_tag="RHW33_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV1645819.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSPRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(27745..28230)
                     /locus_tag="RHW33_002324"
     CDS             complement(27745..28230)
                     /locus_tag="RHW33_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1645820.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFIAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTEYYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(28285..29103)
                     /locus_tag="RHW33_002325"
     CDS             complement(28285..29103)
                     /locus_tag="RHW33_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1645821.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            complement(29202..29435)
                     /locus_tag="RHW33_002326"
     CDS             complement(29202..29435)
                     /locus_tag="RHW33_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV1645822.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(29441..30118)
                     /locus_tag="RHW33_002327"
     CDS             complement(29441..30118)
                     /locus_tag="RHW33_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645823.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTTGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGIRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(30266..30946)
                     /locus_tag="RHW33_002328"
     CDS             complement(30266..30946)
                     /locus_tag="RHW33_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV1645824.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMAGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMTERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(31149..32033)
                     /locus_tag="RHW33_002329"
     CDS             complement(31149..32033)
                     /locus_tag="RHW33_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV1645825.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSHICATHPLNGCTRQAHRLTLQIGERRMTLVDLPGIGETPQH
                     DQEYRALYRQLLPEVDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGETIGKSFDVAVARFSFPAWMLQLLR
                     KARDRIIHLLITLWERLF"
     gene            32218..>33155
                     /locus_tag="RHW33_002330"
     CDS             32218..>33155
                     /locus_tag="RHW33_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645826.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKVEGPKRKRKLVKEREIINSRTKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFNSKAELDALSELYAIYSWVSYCKRCDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLS"
BASE COUNT         8475 a         7773 c         8317 g         8590 t
ORIGIN      
        1 gtacatattt ttttacaggg taaataacag gcaaaaaaaa gcgcggggtg gggccgcgca
       61 aaaagtatga catttttgtt tgcagcaatt tttatgaaaa cgttgccata ctcaaaaggc
      121 ctaaggccag gcggcgtaat gttatttaaa caattacgcc ttcagcggaa tagtggttac
      181 gctttcacac acataacgtg gtaaataccg tcaataattt cagtcccttc agtttcgtgt
      241 tcaaatccag ggaaatgatg gtcccaggat tgcagcgagc gtaaataact tacttgcgga
      301 ctgtttttat cgccgaagtt ttcaccagac agcagcatcg ggattcctgg cggatacggg
      361 ataactgagt ttgccgcgat acgtcctggc agattttcaa tggaaaccag ttcgacattg
      421 ttgtcgacaa tcgcgttgta cgcttcacgc ggggtgattt ccgccaccgg caggccggaa
      481 taggcttcgt tcaaccgtgc gccagggttg ttttctttca gccaggcaaa catggtgtca
      541 cccagatcgt gaatccccat gttcgcataa gtgtcaggat attgttcaac aagttccggc
      601 atcacctgcg ccagcggtgt gttggcgtca tagtggcgtt tgaaggagca aagggtgtta
      661 accagagtcc cccatttccc acgggttacg cccatagaga acaggaacat aatttggaag
      721 tcagtggtgc gggtaggcac aatgccgtgg cgaccaagcc aggcagtgac cagtgccgcc
      781 ggaacaccgg tttcttccag ttcaccatct tcacccattc ccggagcaag gatgctgact
      841 ttaatcgggt cgagcatact ccagttatcc ggaatatctt tgaagccgtg ccagctttcg
      901 cccggatgca ttacccagca gtcctgaacg gtggtcagca gtttggttgg tgcgtcagca
      961 aagtcatagg ttttgccggt ttgtgggtcg gtgacgactt ctttgttcca cggtttgaag
     1021 aaccagctac cgtcagcggt gaactcttta tacagccgcg ccatcgcctg acggaaatca
     1081 accgcttcgt cgatcacttc ctgcgtcagt gacaggccgc tgttgccgtc catcatcgac
     1141 accgccacgt cgttagatgc gcagatggca tacagcgggg aggtggtggc atgcatcatg
     1201 taggcctggt tgaagcggga gaagttaatc gccccacgac cttcacgtac atgaatataa
     1261 gaagcctgtg aaagcgcatt cagcagtttg tgggtggagt gggtggcgaa aacagtagga
     1321 ccgttgtgat cgccaggttc gccgcgcatg gcatagtgat cggcataaat cgggttgaaa
     1381 cgtgcatagc cgtaccaggc ttcgtcaaag tgcagacgat cggaggtttt ttccagcaga
     1441 tcctgcgctt ctttagcgtt ataacacacg ccgtcatagg tgcagttggt caccacgcag
     1501 taagacggtt tttgccctgc tttgtcttta gtcagcgggc tttcactgat tttcttctgc
     1561 aaggtttcag gttgcatttc ctgcggatag attggcccga taatgccgta gcggttgcgg
     1621 cttggcacca tatagaccgg tttcgcgcct gtcagcatca aaccttgttc gatggattta
     1681 tggcagttac ggtcaacaac cacgacatcg ttatcggtca tgcaagcctg catgatggtg
     1741 cggttagagc cggaagtacc gacgactacc gaccaggagc gatcggcacc aaatacgcgt
     1801 gcggcatatt tttcgctttc gccaaatgcg ccagtatggt caagcaaaga accgagggaa
     1861 gttcgttcga tgcccatgtc ggtgcggaac agattttcac catagtagtc atggtagaaa
     1921 cgtccggcgg gtgttttggt aaaaccaacg ccgccctggt ggcctggcgc agcccaggaa
     1981 tattcatgga tgtcgctata tttcatcagc gcgctgaaca gtggcggcaa cagctgctgg
     2041 cggtagcggg tcatcgcggc aacggcgcgt ccggcgataa agtcggcggt atcttccaga
     2101 atccaggcga attcatcgac aagctccagc agatcccggt ccattgcggc gagggctttt
     2161 tcccgatcac ccaacaggaa gaccggcacg ttttgttggc gctcatgaag cttaccgatc
     2221 aattgtctga cgttttgatg ttcgtccgga tgttccattt gatagctgaa catcaggcag
     2281 tcaatggctt cgtttgaaga gagaatggca aaaccatcat caaaggaggt ggatttaatc
     2341 acggtaacat tttgctggct taaagcatct gccagacgct caacggcgtt accgacccag
     2401 gtgtcttgat ggagaaactc gctttcaaca attaatactt tcatcattgc ttacccggtt
     2461 atgaaggaat cttcgttgcg ggcaagtatc ttccggcctt ttcgggctgt aaagcgcgtg
     2521 aaaaacaaac atttttgaaa tatttgtttt tataaacaat gtgttatgtg attattttaa
     2581 attattttca ctgcttatta tcgacggcta aactattttt ttggctgata ctgatatcgt
     2641 ctttagccgg gaaacgtctg gcggcgctgt tggctaagtt tgcggtattg ttgcggcgac
     2701 atgccgacat atttgccgaa cgtgctgtaa aaacgactac ttgaacgaaa gcctgccgtc
     2761 agggcaatat cgagaatact tttatcggta tcgctcagta acgcgcgaac gtggttgatg
     2821 cgcatcgcgg taatgtactg tttcatcgtc aattgcatga cccgctggaa tatccccatt
     2881 gcatagttgg cgttaagttt gacgtgctca gccacatcgt tgatggtcag cgcctggtca
     2941 tagttttcgg caataaagcc cagcatctgg ctaacataaa attgcgcatg gcgcgagacg
     3001 ctgtttttgt gtgtgcgcga ggttttattg accagaatcg gttcccagcc agagaggcta
     3061 aatcgcttga gcatcaggcc aatttcatca atggcgagct ggcgaatttg ctcattcgga
     3121 ctgtttaatt cctgctgcca gcggcgcact tcaaacgggc taagctgctg tgtggctcgt
     3181 gatttgatca ccatgccgtg agtgacgtgg ttaatcaggt ctttatccag cggccaggag
     3241 agaaacagat gcatcggcag attaaaaatc gccatgctct gacaggttcc ggtatctgtt
     3301 agttggtgcg gtgtacaggc ccagaacagc gtgatatgac cctgattgat attcactttt
     3361 tcattgttga tcaggtattc cacatcgcca tcgaaaggca cattcacttc gacctgacca
     3421 tgccagtggc tggtgggcat gatatgcggt gcgcgaaact caatctccat ccgctggtat
     3481 tccgaataca gcgacagcgg gctgcgggtc tgtttttcgt cgctgctgca cataaacgta
     3541 tctgtattca tggatggctc tctttcctgg aatatcagaa ttatggcagg agtgagggag
     3601 gatgactgcg agtgggagca cggttttcac cctcttctca gagaggcgag gggactgtcc
     3661 gaacatcatg aggccgaaaa ctctgctttt caggtaaatt attcccataa actcagattt
     3721 actgctgctt cacgcaggat ctgagtttat gggaatgctc aacctggaag ccggaggttt
     3781 tctgcagatt cgcctgccat gatgaagtta ttcaagcaag ccaggagatc tgcatgatgt
     3841 ctgcacccaa aattacattt atcggcgctg gttcgacgat tttcgttaaa aatattcttg
     3901 gtgatgtgtt ccatcgcgag gcgctgaaaa cggcgcatat tgccctgatg gacattgacc
     3961 ccacccgcct ggaagagtcg catattgtgg tgcgtaagct gatggattca gcaggggcca
     4021 gcggcaaaat cacctgccac acccaacaga aagaagcctt agaggatgcc gattttgtcg
     4081 tggtggcatt tcagattggc ggttatgaac cttgcacggt gactgatttc gaggtctgta
     4141 agcggcatgg tctggaacaa accattgccg atacgttggg gccgggcggt attatgcgcg
     4201 cgctacgtac cattccgcat ctgtggcaaa tttgcgagga catgacggaa gtctgccccg
     4261 atgccaccat gctcaactat gttaacccaa tggcgatgaa tacctgggcg atgtatgccc
     4321 gctatccgca tatcaaacag gtcgggctgt gccattcggt gcagggaacg gcggaagagc
     4381 tggcgcgtga cctcaatatc gacccggcta cgctgcgtta ccgttgcgca ggtatcaacc
     4441 atatggcgtt ttacctggag ctggagcgca aaaccgccga cggcagttac gtgaatctct
     4501 acccggaact gctggcggct tatgacgcag ggcaggcacc gaagccgaat attcacggca
     4561 atactcgctg ccagaatatt gtgcgctatg aaatgttcaa aaagctgggc tatttcgtca
     4621 cggaatcgtc agaacatttt gctgagtaca caccgtggtt tattaagcca ggtcgtgagg
     4681 atttgattga gcgttataaa gtaccgctgg atgagtaccc gaaacgctgc gtcgagcagc
     4741 tggcgaactg gcataaagag ctggaggagt ataaaaacgc ctcccggatt gatattaaac
     4801 cgtcacggga atatgccagc acaatcatga acgctatctg gacgggcgag ccgagtgtga
     4861 tttacggcaa cgtccgtaac gatggtttga ttgataacct gccacaagga tgttgcgtgg
     4921 aagtagcctg tctggttgat gctaatggca ttcagccgac caaagtcggt acgctacctt
     4981 cgcatctggc cgccttgatg caaaccaaca tcaacgtaca gacgctgctg accgaagcta
     5041 ttcttacgga aaatcgcgac cgtgtttacc acgccgcgat gatggacccg catactgccg
     5101 ccgtgctggg cattgatgaa atatatgctc ttgttgacga cctgattgcc gcccacggcg
     5161 actggctgcc aggctggttg caccgttaaa acgcgactaa acgctactgc gccgggggat
     5221 ttattccggc gcacacctct gacgatacca ataacagaag gcgggcgttg gtaacagcga
     5281 cccgataccc tatgagcatt tcaatgacta caaaactcag ttatggattt ggagcgttcg
     5341 ggaaggattt tgcgatcggc attgtgtata tgtacctcat gtattactac accgatgtcg
     5401 tcgggctgtc tgtgggtttg gtcggtactt tgtttctggt ggcgaggatc tgggatgcta
     5461 ttaacgatcc gattatggga tggattgtaa atgctacgcg atcgcgatgg ggtaagttca
     5521 aaccctggat cctgatcggt acgttggcaa actctgtaat cttatttctc ctctttagtg
     5581 cgcatctgtt tgaaggtact actcagattg tctttgtttg cgtgacctac atcctctggg
     5641 gcatgactta caccattatg gatattccct tctggtcgct ggttccaacc atcacgctcg
     5701 ataaacgtga gcgtgaacaa ctggttcctt atccacgttt ttttgccagt ctggcgggct
     5761 ttgttacggc aggtgtgacg ctaccatttg ttaattatgt cggcggtggc gatcggggat
     5821 ttggctttca gatgttcaca ctggtactga tcgccttttt tattgtttca accatcatca
     5881 ctctgcgcaa tgtgcatgaa gtcttttcgt cagacaatca accgtctgct gaaggaagcc
     5941 atctgacact taaagccatc gttgcgctta tttataaaaa cgatcagctt tcatgcctct
     6001 tgggtatggc tcttgcttat aatgtagcca gcaacattat taccggcttt gctatctatt
     6061 atttctcata tgttatcggt gatgcggatt tgttccccta ttatctgtcg tatgcgggag
     6121 ctgctaacct ggtgacgtta gtattcttcc cacgcttagt taaatcatta tcccgacgca
     6181 ttttatgggc cggagcatct attcttccgg tgttaagctg tggtgttctc ctgttaatgg
     6241 cattaatgag ctatcacaac gtcgtcctca ttgtgattgc gggtattttg ctgaatgtgg
     6301 gaacggcgct tttctgggta ttacaggtca tcatggtggc agataccgtt gattacggtg
     6361 aatataaact gcacgtacgc tgtgaaagca tcgcttactc cgtgcagact atggtggtga
     6421 agggcggttc agcctttgcg gcttttttca ttgcggttgt gttagggatg attggctatg
     6481 taccgaatgt tgaacagtct acgcaagccc tattaggtat gcagtttatt atgattgctc
     6541 taccgactct gtttttcatg gtaacgctga ttctctactt ccgtttctat cgcctcaatg
     6601 gcgacacgct gcgcaggatc cagattcatc tgctggataa atatcgcaaa gtaccgcccg
     6661 agcctgttca tgctgatatt ccggtcggtg tagtgagtga tgtgaaagcc tgacgtggac
     6721 aatagctaac gaaaatggcc tgatgcgata tgtttatcag gccacgtctt aatgtaatgc
     6781 tttggatttg caaattttgt agaccggata aggaattcac gccgcatccg gcatcaacaa
     6841 agtgcacgtt gttatccggt tatcaagcca aagcgccgta gctgacggca atgaaagcga
     6901 gcgaggaata tcaccgtggt taatgcgcca agcagcgcgc agaacatatc ggattgcgtg
     6961 tcccactggt cgccctgggt gccgagaaaa tcatccgctc cctggcccat tgccagcgcc
     7021 gcccaccact cgattaattc atacatcgcg cttatcgcca gcgccacgca acagaccaga
     7081 aacgccacca ttttacgtcc gcgaacgtac atcccgcgca caaggatctc tcgtgccacc
     7141 agtgcaggca ccagcccctg gaaaaaatgc cccagcttgt catacggatt acggctcaac
     7201 cccagccatt cctgcacctc aaaaccgatg gggactttcg cgtaggtgta ttgcccgccg
     7261 accatcagga tgatggcgtg aaagaaaatg agcgtataga gcagcggcgt taacggataa
     7321 cgtttagcgg tggcaagcag tagcggcacg acaataatca ccggtgtcac ttccatcagc
     7381 caggtgagtt tgtcatgggc cgaaatgccg gtataaatca ggattaacgt tagcgccagt
     7441 gcgccggtgt taagaattaa cggcttgagc gtgcgggtca tggtgagttc gcaagtcagg
     7501 gaaaatactg actattcacc agcaagcgtg aaattacaat cgcaaaagag gtggcccagg
     7561 gggatcacct ggcagcatgc tgccaggcgc tgggccgaag aggttactta gtgcagttcg
     7621 cgcactgttt gttgacgatt tgctggaaga agtcgttacc tttgtcatcg accaggataa
     7681 acgccgggaa atcttctact tcgattttcc agatagcttc catacccagc tccggataag
     7741 cgacgcattc cagatgcttg atgctctgct gcgccagtac cgccgccgga ccgcctatgc
     7801 taccgaggta gaagccgccg tgtttatgac acgcgtcggt aacctgctga ctgcggttac
     7861 ctttcgctag catgatcatg ctgccgccgt gggattgcag cagatccaca taggagtcca
     7921 tacgacctgc ggtggttggg cctagtgaac ctgatggata accggcaggg gttttcgccg
     7981 gacccgcgta gtagatcggg tgatctttga tgtactgcgg aagttcttta ccggcgtcaa
     8041 tcagctcttt cagcttggcg tgtgcaatat ctcgacccac aataatggtg ccggtgagcg
     8101 acaaacgagt ggaaaccgga tattgcgaaa gctgggcgag gatctctttc atcgggcggt
     8161 taaggtcgac cttcaccgct tcgccttcac cggcctggcg cagttcttgt ggaatgtact
     8221 gacctggatt gtgttccagt ttttcgatcc agataccttc gcggttgatt ttcgctttaa
     8281 tgttacggtc agcggagcag gagacgccca tgcctaccgg gcaggatgcg ccgtgacgtg
     8341 gcagacggat aacgcgaatg tcgtgcgcga agtatttacc gccaaactgc gcgccaagac
     8401 cgagtttctg ggcctcttcc agcagttcct gttccagctg gacatcgcgg aacgcctgac
     8461 catgttcgtt cccttccgtc ggcagttcat cgtaatagtg agcgcttgct aacttgacgg
     8521 ttttcaggtt ggtttccgca gacgtaccgc caatcacaaa cgcgatatgg tacggcgggc
     8581 aggctgcagt accgagggta cgcattttct cgacgaggaa gtttttcagt ttgccgggag
     8641 tcagcagggc tttggtttcc tggtagagat aggttttgtt ggcagagccg ccgcctttcg
     8701 caacgcaaag gaatttgtac tcatcgccat ctaccgcgta caggtcgatt tgcgcaggca
     8761 ggttagtgcc ggtgttgacc tctttgtaca tgtccagcgc cgcgttttgc gagtagcgca
     8821 ggttatcctc gatataggtg ttatagacgc ctttcgacag cgcttcttca tcaccgccac
     8881 cggtccacac gcgctggcct tttttaccga cgatgatcgc ggtgccggta tcctggcagg
     8941 tcggcagcac gcctttggcg gcgatttcgg agtttcttaa gaattgcagt gccacgtact
     9001 tgtcgttttc gctggcttct ggatcgtgaa gaatagccgc gacctgtttc tggtgtgccg
     9061 ggcggagcat gaaagaagcg tcgtgaaagg cttgctgcgc cagcagagtc agggcttctg
     9121 gttccacttt caggattgtt tcgccgtcga agtcggcaac gctaacgtaa tcggaagtga
     9181 gtagatagta ttcggtattg tctttcccca tcgggaaagg tgcctggtag ataaagggtt
     9241 tgtttgacat agcttccagc ctgtaactct gtatttgtta ttcgaaaatg gcgtgccgcg
     9301 tagtcacggg cggcacgcaa agtgcattta taagaacccg tacatcgcgg cgaagatcca
     9361 gccgaagacg cacgatacgc tcacaccaat caaccccggc agaataaagc tgtggttgat
     9421 gacgaagcga ccgatgtggg tggtgccgga acggtcaaac tgaatcgctg ccagatcgct
     9481 cggataagtc ggcaggatgt aataaccgta gcaagccggt gctgaagcca cgatgtatgc
     9541 cggatcaacg ccgatcgcca gcgcgaccgg aacaatcgcc gccagcgccg cagcctgaga
     9601 gtttacaaac ttggaaacca gcagcagaac aatggcatag gcccacggat actctttcac
     9661 catttcaccc agtacgccct gaatttcaga catatgcgca ccgaacatgg tttctgccat
     9721 ccatgcgata ccgtacaccg ccacgatggc gatcataccg gaacggaaga cttcgttttt
     9781 tgagatagac gcgggattgg ttttggtcag gataataatc agcgccccgg tcagcagcat
     9841 aaacatctga ataaccagta ccatcgacag cggtttgccg ccgaaggatg gacgcaggtc
     9901 cgaatcagca ccaagaagtg cgactacagc gattgccccg aggaaaatcc acattgccag
     9961 ccagttgctt ttcggcagtt ttttatcgag cagcgtcgcg gtatcaccgt aaacatactc
    10021 acggttttcc ggtacggaga tgaatttctg gaactcttcg tctttatcca gatctttacc
    10081 gcggaaccag ctgaagatac cgatcgccag gataccgatt aacgtcgatg gaatggtgat
    10141 tgccagcagg tcgaggaact caagatggcg accatcaaag gtgacattac ccagcattgc
    10201 aaccagagac acgaccgcaa ccgacaccgg actggcgata atccccatct gtgcaccgat
    10261 agaacttgcc gccatcggac gttccggacg gatgttgttc ttaatggcga cgtcgtagat
    10321 gatcggcaga atggtgtaaa ccacatgacc cgtaccgcaa agaatggtca gcgtacaggt
    10381 cacaaacggc gcgacaattg agacatattt cgggttgcgg cgcagcagct tctcggcaat
    10441 ttgcagcatg acatcaagac cgcccgaagc ttgcaaggtc gccgatgccg ccaccaccgc
    10501 aatgataacc agcatgacat caaccggtgg tttacctggc tgaaggtgga agacgaagac
    10561 cagaatgacc agaccgatac cgcctaataa acccagcgcg ataccaccct ttctggcacc
    10621 ataaaacaga catatcagta ttatgataag ttggatagta aataacatgt gtgaaccctc
    10681 gcgataatcc tatttaaatt tttgctgaat agatcacagt cacgttctgt tttgtatgaa
    10741 ctgttttcag agcctgacta aatatccgtt tggtcgttac cggctttagc aaatacctca
    10801 cagtgaatat tggctgatta aagcagcggc aaatttatgc actgtgataa gcggcttttt
    10861 cagagagaaa aactctctgg cgatgggttt aaagtttgta ctgcgcagat gttaatgttt
    10921 tgtttgctgt tatcttattg atatttatcg gtctattttt tgttctcagg gcagatatca
    10981 ttaggtttat agattaatct gatctaccca tttgtgggta aaaatacaca taatgcgggt
    11041 gacataatag ttaattaact tttgttagcg ttttgaaatt aaaaacaccg ttcacctgaa
    11101 gagatattaa tttttagcga tgatggaggg ataattatat ttgatctggc acaagtttta
    11161 ctgatgaagg atgtaacttg tgccaggggt attttgcatt acggtaataa ttattactta
    11221 cagataacca gcgatccgtt attggcaata ttgtttcagt agtgagtagt gttctgcctg
    11281 aatacggtaa cggtaaactg gacgccccgt gacgccataa tggatactgg tgaacaagat
    11341 gtggcagttg accagccaga tgaggtattt acggcaggaa acacgcgaaa tgttaacctc
    11401 gttggctagc tcgtcggttg aaaactcata gtcctgatgc gcgtcaatcc actggcacag
    11461 tgtgcgtaac gtctgcggcg ttaagccttt tggcaagcga cgaggatcct gctcgttgga
    11521 gctgctgccg tggattagct gatcaagctc ggcctggtca taatactgat gtttttccag
    11581 cgccattttc ttttgccgcc agccggtgag cgcctcttca aagcgggaag cctggaaggg
    11641 tttgatcagg taatccacga caccgtaatg cagcgaatct ttaatggttg ccgcatcggc
    11701 tgcggaggag atgacaatca catcactttt gcaacgcgcg ttatgcagga caggcagtaa
    11761 atcgagcccg ttctcttttt gcatatagat atcgagcaat atcaggtcga taggcgtatc
    11821 gctattgaag ataatctctt tggctttctc cagcgtcgag gctgttccac agcattgaaa
    11881 gcctgggatt tgtgctacgt atcggcgatt cagctccgcg accattgcgt cgtcatcgat
    11941 aattaataca ttgatcatct gttcgacctc tccccgtccc agggtatctg gacaaaaaat
    12001 tgtgtgaaaa tcccgggttc cgattccacg gcgatgctgc cgccgagatt ttctacctgt
    12061 tgtttgacaa gtgctaaacc gacgcctcgc tcgcttcctt ttgtcgagac acctttgtca
    12121 aaaatgtgat cgattttgtc gggtgcgatc cccggtccat catcgttaac ttcgcagtgc
    12181 agccagccgt gacggtagtg caatgttacg ctaatttcgc caccgggttc cggccctaat
    12241 gcttccagcg cgttttctat cagatttccc aatgtggtaa tcagcgtcgc gacctggtcc
    12301 tcactgccgc tgtctggcag ctggctttca ctgtttaaaa tcagcgtatg gcctaaatcg
    12361 gtcgcgcggt taatcttgct gattaaaaaa ccagcgataa ccggagattt gatcttaccc
    12421 agcagagagc caatctcttc ctgatagtta ttggctgttt tgagaatgta atcttccaac
    12481 tgcttataac tcttcagatg caataatccg agaatcacat gcaatttatt cataaattcg
    12541 tgggatcgtt cacgaagtgc gtcagcatag ttgaccagac catcgagtcg ctgcatcagt
    12601 ttacgtactt cagttttgtc cctgaaggtt gaaatggcac cgatgataac gccattactg
    12661 cgcaccggaa cggtgttgat cagtaatagc cgatctttaa tcgtaatctc ttcgtcgcgg
    12721 cgcggggtac cgtcgcgtaa cacttccgag acatctacca cctgtgacca cgagtggctt
    12781 agcgtcgaca gtttctcatc gtcctgcgac ttacggtaat tcagcaattc ttgtgcggca
    12841 tcgttgatca gcgtgacctc gccgcgatcg tccacggcaa cgacgccttc tttgatagac
    12901 tgcaacatgg cctggcgttg ctcaaacagc gtggagattt cgtagggttc caggccgaaa
    12961 aggatttttt tcagtacttt aaccagaatg caggtgccaa tcagtccgac cagcatgcca
    13021 aataatactg accagataat gctccagcga ctgtcattga tctgttgggt cacgcggctt
    13081 aactcaaggc cgatcgccac cacgccaatt tgtttatgat tttcatcgta gatgggggta
    13141 aatacgcgta aagcctgcgc cagaaaaccg cgattgatag cgacattttc ttcgccattc
    13201 agcgctttaa ggatgtcatc acctttaaat ggctgaccaa tacgctgggc ttcaggatgc
    13261 gagtagcgaa gactttgcat atcggtaacg acaataaaca gcagatcgtt gcgtttgcgt
    13321 acggcttccg cgatggcctg gatgccgctc tcctgcggtt ttttctgcaa gccctgacgg
    13381 atttccggcg agtcggcgag agtacgcgcc actgccagtg ccttgttggc tagcccgtct
    13441 cgcgtcatat cactgatttg cgagaagtaa atcagatgca ccaccaatag caccgagaac
    13501 agtaccgcgc tgaccattaa gatcactgtg gtactcaatt tcatcggacg tttgcgtaac
    13561 atgcggtagg gcaatgaatg tctcatcagc ttccttgtgt gacaaatttc ttaagcatta
    13621 tctctgatga ggcgggtaat tcaaagggag taagaatgat tggctatata ggggaagaga
    13681 ctctggcaac ggaaactgcc agtgctgtat gaagattcca gggctatgct tatagtgaaa
    13741 accatactga tgagagaggg aaggtcatgg atcaggcgct actggacggg ggttatcgct
    13801 gttataccgg cgaaaagatc gatgtctatt tcaacactgc gatatgtcag cattctggca
    13861 attgcgtacg tggcaacggc aagttattta atctcaaacg aaagccgtgg atcatgccgg
    13921 atgaagtcga cgttgccact gtggtgaaag tgattgatac gtgcccgagc ggcgcgctga
    13981 aataccgtca taaataagcg agggtaaaat ggaaatacgc gaaggccaca ataaatttta
    14041 cattaatgat gaacagggca agcaaatcgc tgaaattgtc tttgtgccga ccggagagaa
    14101 tttagcgatt atcgaacata ccgatgtcga tgaaagcctg aaagggcaag ggatcggtaa
    14161 acagctggtt gcgaaagtcg tggaaaaaat gcgtcgggaa aaacgaaaaa ttatcccact
    14221 atgcccattt gcgaaacacg aatttgataa aacgcgggag tatgatgata ttcgcagttg
    14281 atggaagagt acagagccac gatatttttc atgctctccg cggagtgatg caggagagca
    14341 tctgaagggt agggggatgc acaaagaatg ggcagagagc gcgttttttt gtcccaagtc
    14401 atccccttta ctgagcaaaa aaaagaatat ctcctatatg agaatcatca atcggggtta
    14461 ataagttttg cgtccccaga gtgttcaata ttgataggag tcatattatg gaaggtaaaa
    14521 acaagttcaa tacttatgtt gtttcttttg attatctatc atcttattcc tcagtgttct
    14581 taaggttaag atcattgatg tatgatatga atttctcctc tatcgtggct gatgaatatg
    14641 ggataccacg acaattgaat gaaaactcct tcgcaataac gacatcgtta gccgcaagtg
    14701 aaatcgaaga tttaatcagg cttaaatgct tagacttacc ggatattgac tttgacctca
    14761 acattatgac agttgatgac tatttccgtc agttttacaa atagccagga aggataagaa
    14821 gaacataaaa aatggcacta ttctctaaaa tattaatttt ttatgtgatt ggtgtgaaca
    14881 tatcctttgt cattatctgg tttatctcac atgagaaaac acatattcgt ttacttagtg
    14941 cattcctggt cggaataacc tggccaatga gtctgcctgt ggcattactt ttttctctct
    15001 tttaggagct gtagttgagt ggacccgctg gttatcgtga aacgaagcgt ctctcctcaa
    15061 aactggattt gcggaacgat gaacgaagca gtcgcctgac tgcttcgttc attaaagtga
    15121 aatttatttc tgtgggcgca tcgccgggaa gagaataacg tcgcgaatag tatgactgtt
    15181 tgtaaacagc ataatcattc ggtcgatacc aatacccaga ccagcggttg gcggcagacc
    15241 atattccagc gcagtcacgt aatcttcgtc atagaacatg gcttcgtcgt cacctgcagc
    15301 tttagcgtta acctgttcct ggaaacgttc agcctgatct tctgcgtcgt ttaattcgct
    15361 aaaaccatta ccgatttcac gaccaccgat gaagaattca aaacggtcgg tgatttccgg
    15421 gttaacatca ttacgacgcg ccagcgggga cacttctgcc ggatattccg taataaaggt
    15481 tggctgaatc agatgtgctt ctgccacttc atcaaagatc tccgtgacaa tacgtcccag
    15541 cccccagctt ttctctaccg taattccgat agattcagct aatgctttag cagcatcaaa
    15601 attatccagg tcggccatat cggtttctgg acgatatttt ttgattgctt cgcgcatggt
    15661 gagtttttca aacggtttgc cgaaatcaaa cacatgctcg ccataagtga ctttagtggt
    15721 accaagaacc tcttgtgcca gggtgcggaa cagggactct gtcagttcaa tcaaatcgtg
    15781 gtaatccgca tacgccatgt agagttccat cattgtgaac tcaggattat ggcgaacaga
    15841 aataccttca ttacggaagt tacggttgat ttcgaatacc cgttcaaaac cgcctacaac
    15901 cagacgtttc agatacagct ccggcgcgat acgcagatac atatctaaat ccagagcgtt
    15961 atgatgggta ataaacgggc gagcagatgc cccacctgga attacctgca tcatcggggt
    16021 ttctacttcc ataaagccgc gcgcgaccat gaactggcgg atagcggcca gaatttttga
    16081 acggacaaca aacgtttgac gggatttatc gttagcgatg aggtccagat aacgttgacg
    16141 ataacggact tcctgatcct gcagaccatg gaatttatct ggtaaaggac gtagtgcttt
    16201 agtcagcagg cgcaactcag tacagtgaat ggaaagctca cccgtttgcg tcttaaacag
    16261 cgtaccgcgg gcaccgataa tgtcacccag atcccatttt ttaaactgat cgttataaac
    16321 accttctggc aggctatctc ttgcaacgta cagttgaata cggccaccga catcctgcaa
    16381 cgttacaaag gaggctttcc ccatgatacg acgggtcatc attcggccag caaccgagac
    16441 ttcaatgttt aaggattcca gttcctggtt atccttcgca tcaaactctt cgtgcaactg
    16501 gtcagaggta tggtcgcggc gaaaatcatt gggaaacgcc acaccttgct gacgtagtgc
    16561 cgccagtttt tcgcggcgat ttctcagttc atcgttaaaa tcaatagcct cattggctcc
    16621 ccgtgtttct tgttcagaca ttttggttcc tctaaatcca gctttcaatt tagcgtagat
    16681 aaagagacag atcggtacac tgtattatct gcctcgacta ttaataactc agtttatctt
    16741 atcgaaacta acgcttacct caagttgata tcactaataa ataaccatca aaatggttta
    16801 aatgacttat taatatcgat ttgctagtgt tataaactga aagttaattt attcataact
    16861 aattgttttt acgacagtta atcgttgctc tcctgtatca tattcgtcgg cgtgctaaac
    16921 aggaaacggg tagcaaaggc caatacgacg atgatcgcga cacaagccaa cgtccactct
    16981 cccatctgag aaaagaatcg ctggtaagct gcaattgcca taccgctaat ttgcgactct
    17041 gtcgtctgct gtgctactac gcctgccagc cagttggcga ccgcgcctgt tgccagcata
    17101 taaatcccgg ttaatacgcc agacattttc agacgcgtga tttgcgcaat cgccaccggg
    17161 tcaataaaga gttcggcaaa gcccattagc gccaacccgg atatcatcac gcccattgac
    17221 gcttgaccgc cagccgctgc atgtcgggca tcaaatgcca gcaacataaa gccacaagcc
    17281 atcagaagta agccaaaggc aaacttcagc cagacgcgca atgttgagtt gccgcggctt
    17341 tctggcgatg ccagccaggc cagtacaacc ccagcgagca tcaccgcaat tgcattcacc
    17401 gactggaata gtgctgtagg tacttcaata ttgaatgcct gacgattcac aaagcgatcg
    17461 ataaacagac tgatggtact gccgccctgt tgtgccagta cccagaacaa tgtcccgaca
    17521 aacatcaaca atacaatttg ccaaagagca cggcggtgtt cggggaattt gatcatcatg
    17581 cgggcaatga tttgtgcggc aatgaggcaa acgatcgcca gcaaatatcc ggaccagtcg
    17641 ttctccagca gcagggtaaa aaatactggg gctaaacaga gcatcaccac taaccagctc
    17701 catactggta aggcaaattt gacgctagtg agcgcttttt tatccatact acgcgtggac
    17761 tggaaatgac gatgaccgct taagaaaatc aacaaaccga taaacatgcc gccacccgca
    17821 agggcaaagc caacatgcca tccataccac tgagcagcca ggccgcaggc gatgggggcg
    17881 gcgatagaac cgatattgcc cgcagcatac agcagcgaaa aaccgccatc acgtcgatga
    17941 tcgttctcgt cgtagagctc tccaagcaaa cagctgatgt ttgatttgaa taaaccgtag
    18001 ccacaaataa tgattgccag cgccagatac aggctaaagg ttgaatttgt atcaatgccc
    18061 agcaccacat ggccaagggt cattaacagc gcgccggcaa ttactgcagt gcggttgccg
    18121 agcaggcggt cggcaagcca gccgccgaga ataggggtaa cgtaaaccag agaagcatat
    18181 gcgctgaaca ggctgatggc atggttatca tcaaaaccaa gctgatgggt gagatagaga
    18241 atgagtaagg cacgcatgcc gtaaaaactg aagtactccc agatttggat cgccacgata
    18301 tagtatatcg cgcgcggctg tgagggtgtt ttcatgtgtt ctccttatga gcaaaaaagg
    18361 gaagtggcaa gccacttccc ttgtacgagc taattatttt ttgctttctt ctttcaatac
    18421 cttaacggta tagcggccat cagcctgacg gtatgcaccg tgaatatcgg tttcaaagcc
    18481 cggatagtga gcgccgattt cacacagcat ctgcaggaac tccagaaccg gacggctttc
    18541 ttcggtgatc atttcacccg gcattaccag aggaactccc ggcggatatg gaaggatcat
    18601 attggcgtta atacgaccta ccatttcgtc gaggtaaact tcttcggtca taccgtgcag
    18661 ctctttctgg aacgcagcat acggagtcat taccatcgtc ggcagcactt caaatgcgcg
    18721 atacatcaga tccggcagat tgtggtgaac aatcagtttg tggatattct gagccagttc
    18781 ctgaatacgc atgttttcat agaattcagg atcttcacga tacagagacg gcagcatgtt
    18841 tttcacacgc aggttcaggt cgaacgcacg tttgaagtca gtcagagcac gcagcaggct
    18901 cagtgctttg gtcttatcga taccgatgct gaacaggaac agcaggttat acggaccggt
    18961 tttctcaaca acgatgccat gttcgtcgag gtatttcgcc acgatgctgg ccggaatgcc
    19021 aaagtcgctc atggtgccgt ctttttccat ccccggagtc agcagggtga ctttgatcgg
    19081 gtcaagatac atgtgctcgt tatcgatgtt tttgaagccg tgccaggtgc tgtcagaacg
    19141 cagcggccag cattcagtcg tatcgatatg atccggctgc catacatcaa agaaccagcc
    19201 atcagattct gttctcagac gtttgatctc tttacggaat ttgatcgcac gttcaatgga
    19261 accgttgatc agacgcttac ctgcattgcc tttcatcatc gccgcagcgg tttcagtgga
    19321 cgccacgata ccgtagtgcg gagaagtggt ggtgtgcatc atgtaggctt cgttaaaggt
    19381 ttcttcgttt acgtcacctt taacgtggat catggaagcc tgagagaacg ccgccagcag
    19441 tttgtgagta gactgggttt cgtaaatcac tttcccttct acacggccac cgctcatacc
    19501 gcatttacct tcgtaaatcg gtgagaagtt ggtgtaaggc acccacgcgg agtcaaagtg
    19561 gatggatttc acatccagtg ttttcttgat gaagtcggtg ttgtacagca gaccatcata
    19621 ggtagagttg gtaattacag catgtaccgg ccaggttgcg tttggtgttt ctttcacgcg
    19681 cttagcaatg gtagcgtgct ggaattcact ctgtgggata ccaccaagaa taccgtaagc
    19741 gttacgggtc gggcggaaat agattggcgt aacatcgctc atcatcatca ggtgggtcag
    19801 cgatttgtgg cagttacggt caatcagaat ggtgctgcct gccggagcag agtacatacc
    19861 aacaattttg ttcgcagtgg aagtaccgtt ggtcaccatg tagctgcggt ctgcgttaaa
    19921 gacgcgagcg atatactgtt ctgcttcttt gtgtggacca ctgtgatcca gcagagaacc
    19981 cagttcagat actgaaatgg aaatatcaga tttcatggta ttcggaccaa agaaatcata
    20041 gaacaggcta cctaccgggc ttttctggaa tgcagtaccg cccatgtgac caggagtaca
    20101 gaaagtatat ttaccttcac gaacatattt aaacagtgct ttagtcagcg gaggcagaat
    20161 agtgttgata tattcgtcag tggtctgctt gatcttgtta gcaatatctt cagcagcacc
    20221 cagcgcatat tcaaagaagc taatctgtaa acgcaggtca ttcaggctta catcgagagt
    20281 ggaatacgta ttagcgaacg cgtacaacgg caggttctcg ttcattttgc taatttcttc
    20341 gcacagctcg agattatatt tatcccagtc aaaaataacg ccgcacagac gcgcattgtt
    20401 ttcgatcagt tttaataagt cgtcacggtc gttcgggtaa acaatctgga agttcagacg
    20461 ttcaagcgcg cgatgaagtt cacggatggg ttcttcttta aaataaaccc ccatgtgatt
    20521 caatattgca ataacgttca tagtcatatc tccaggtaaa aaaggcccct cccaacacat
    20581 gggacaaaat gaaaggagga gcctcggaaa atacttttaa ttaatgtgcg ttagacgcgg
    20641 tgtggttatc cattgagtgg ctctggcgct cgtgcatttt gcgagcgtag aacatcagga
    20701 taatcaggct gacgatgaag gtacctgcca gctcgaagga gcttgcgccc atcagcgcga
    20761 tgaagcagaa cacgcaaccc agtacagagc agatcaggct gacaaagttg cggatgttaa
    20821 cgccttcaaa acgaatcagg tcaacgcaag agtagaagta cggcagcata gtcagcagta
    20881 ctgcgatacc ggtcagttca ccgaacaggt cagatgcttt accaccggca gagttcatca
    20941 gagtgatgag gatcatcagg gcagtcattt tcactgcagc cagcagcaga ccttttttcg
    21001 gaataccgtt gctgtcgact tcaccataaa ctttcgggaa gttaccgtcg ttagcggcac
    21061 gtacacctgc ctggcctacc aacatcatcc aggagcccag agaagtcagg cacgcaaagg
    21121 cggtgaatgc agaaaccagc ggcgcagccc agttaccgag gatagttgaa gcactgattg
    21181 caaacggagc accggaagcc gccattacag aagacggata cataccggaa agcacctgag
    21241 tcgcagcgat ataaacaata cctgctaaac cagtacccag catggttgcc agcggaacgg
    21301 tacgtttcgg gtttttaacc ataccagtac ttactgctgc ggattcaaca cccacgaagg
    21361 cccacaggca gagcagaata cttttaatga tcgcatgacc atcagtggta tccgcagtat
    21421 tccagttagc tgcataagtt gccgcatcaa accaatgcca gccaacaata gcagttatca
    21481 ccacaggaat aagaaccagc accagaccaa tagtggttaa acggcttacc caagtaccgc
    21541 cgagcatatt tacaaaggta aatacccaga cgatagcaat acaggcgata cccgccggaa
    21601 caggatcatt taatactggg aagaaggtgg aaagataaga tacagcggta ataccaatcg
    21661 ccaggttacc aatccagtta gcatggtaat aaagaacacc tgtctgaaaa ccaaatgcag
    21721 gagaaatttc tccggcataa gcaattgggc caccttgttg cgggtttttt gttgccagtc
    21781 gggcatatac atacgccagc gacattgcac caataataga gataatccaa ccccagatag
    21841 caataccacc gatacttgct aggttcgcag gtaataatgc aataccgctc cccatcatat
    21901 taccggcaac aacaccggta caggcaaata gcccgatctt cttggcagaa ctcatgctct
    21961 tctcctaatt tcatttttga atttggagtc cgggtcatga tgtataacta tttcctgacc
    22021 agaccaaact ggcgataaga ttactcacga aaaaaggatt aatcctaaag attaggtgaa
    22081 ataacacaaa agtttctgta agtgagaatt tgaggttttt tattaacaca tcaggatcgc
    22141 aagttgatat catgaaaaga taaacattca atgtttacaa tggattgcgt gatattgtct
    22201 ggttaaattt atgtaataaa aattatgcgg caaataaatt gccgcagcat attataccaa
    22261 caggaacata caaaaactca acaacaaata ttcccgagca tgaatcaacc cgggggttac
    22321 ttattctgaa gcgagaaatt tgtcgagata aggtacaaca taaggaacag aagtctggaa
    22381 tataccattt tcaatccagt aaagggtgtt tgcccctggg cgtaaattaa aggcggtgag
    22441 atatgcatca gccgcttccc ggttcatccc cttcatttca taaaccttgc caagcaatac
    22501 ataatttatc caggacattt caagatcaat gccagtattt atcgcctggt aagactcatc
    22561 tgttttacct tttaccagag cactgaccgc ttttatttga tatataatgg acaggttatt
    22621 caattccggt agtgtaacaa tgttatctat ttctgtgttc agtgctgcta attgtttttc
    22681 atctaaagga tgttgagaat ggcgcacgat atcaactaat gctttttctg ctctcgcgta
    22741 ggtaaattct ggggatgatt gaacaatctc acctaataat tcactggcac gattcaatga
    22801 tttatcatcg ccatgcagta aataatcatg tgcctgataa aaattagtta ataacgcacc
    22861 acgatgaggc aaaatttgct ggagcgtctc ctgcattcgt tgtggccacg gttggtttaa
    22921 cgcttttgat aaactctcca gtaaatcatt ttgaatcgcc agctgattac cgttagtgat
    22981 gacataacgt ttatccagca tggttgaacc atctgcattg tctaccaatt ttatcgacat
    23041 aaagcattgt tgagcacggt attggcgctg attaacaaac gcaatagata atgttttacc
    23101 ggaactgctc ggttcatcaa tgttgtagtt gattttgtca tgcaccataa aggtggagaa
    23161 ggtgttaagt gatgtcgcca ccaaatcacc cacgcctatc gcgtaagaga gctgatacgg
    23221 ggaactccag ctgttacaac ttttatttac catattaatg tcaatatcgc gtggattgag
    23281 caaaatacgc gatttgctca taggaagacg tgtatcaaga cttgaaaacg ctaccagtgc
    23341 tacacagata cctaaggaca acaggaaaaa aaaccatacc caaaaggtag tgaatcgttt
    23401 gcttttaact ggggattgtt caggtggcgt tgcggtgttt tgaatgttaa gactgtggga
    23461 gggagaatct gtggcaggaa ccgcctctgg tataggggga ggcgaagata gcattatttc
    23521 ctctccctct tcttcgctgt accagataac cggcaccatt aatttatagc cgcgctttgg
    23581 tacagtagcg atatagacag gactatcttc atcattatct tttaatgact tacgtagttc
    23641 tgagatactc tgcgtcacaa cgtgattggt gacaatactt ctcttccaga cattatcgat
    23701 aagttcatcc ctgctaagta cttcgccact gtgttgagca aagaaaacca gaaggtcgat
    23761 taatctcggc tcaagggtaa gttgacgccc attgcggcta atttggttta tggacggagt
    23821 aacaagccat tcgccaacgc gaactacagg ttgttgcata atagaaactc attcgaaaag
    23881 ggaatgatgc aatgataatt gccacaacct atttttacca tctatagatg ggtttattta
    23941 catattattg gtgaatgcaa gacgttattt ttaccagcca taaacttctt gattacatgg
    24001 tattacgaaa ggattttact gagaaccaga agtaatattc cttaccatca aaattcatca
    24061 tctttgccaa agaaaaatgt tcagaaaata atccatggaa aattgtccgg agcgcttact
    24121 attttaatgg attgttagtc tttgcatgag caagcggact gatacatttc tctttgttct
    24181 cattcagaaa atctcatcag tcgccgttca gaccggtgtt gtgctttatg gatgcgttac
    24241 gctcctgatg acgtcatttg acgttcaaca gcatcacggg gtcgcacgac atttcacgtc
    24301 agttagtgct atcgctcagg aacaaatttt cccgaattgg gatatgcccg caaattgctg
    24361 gtgatgtggg gagaatctgg ttgagttcgg tagaattgat ttggaggcag aacgcttaaa
    24421 tcgtggcgtc ctgaaacgaa aaacggacct ccgtggaggt ccgtttatat gaatttggtg
    24481 ccggactcgg aatcgaagaa tgcttctatc atgttgatat tattttattt tgtttggttt
    24541 ggtgaaatat gtaccctctt ttgtacccgc agtccctttt gatatcgaaa gggaatcaaa
    24601 ctcggggtca ggctgagagc acagaaggaa agtgcctggc tattatatcg ttaattctgt
    24661 caatcagctc cagccttttg aaagtgatgt gtgcagatcc acccttctta aagtatttaa
    24721 tgatgaacat ctcatcttca tagcgtcctt taccctgaac ggactgtatg tggtcatcca
    24781 gattaatggt gatattccct cggttgtcgg ggatcggttt accgctgagc agatgcaaca
    24841 ttctttccag gtcagccagg cgatctgcct gcattccact gataagatga aatccccact
    24901 ggtcccatct caccagattg ttgacgataa ttttactgcc aaatttacag ggtgagttgg
    24961 ttttgtaatc ccagctcagc cctttgaaga cgttgatcac tccccgttca aacacttcat
    25021 ctttgttctg gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg atttccggaa
    25081 aattatcata ttccagtgac ctggacctga ctgaggtgga gcagataacg tccgtattct
    25141 cgttgaccac ttccggacgg gagactgtga tttcagacat aatcgatatt cctttttgca
    25201 gataaatgaa aacgccagcc gtgttcaggc tggcgtcagt ggaatgaagt ctggtgagtg
    25261 agatgatgag tgagactcac cggttctggt gctgaagctg cagttggcgc agggtacgga
    25321 tatcctctct ggcctgttcg atacagggca acagtgctgc tgaatccgtt ttatcctcat
    25381 tgttgtcaaa gataattccc gattcgcagt cgatattgtc ctgcagccac gcaatcagaa
    25441 tatccagcgc tgtttccgtg gttaatgatt tcatgttgtt tctggttacc agtcgaaagt
    25501 gggtaaaccc ggcaggcatt cagcgttggc atccagatga atgagactga cgccataacg
    25561 tcgggtaagt gtgacaacca gacgacggaa cgtttttgac aggcccaggc gcttaagccg
    25621 cagaaccggg tacgaccacg catcagtacg taacagataa ccggtacctg taaaatgaat
    25681 ccattctgat tcaccaacgt ccctggtctg gtgtgacagc gagtacaacc aggcgttgtc
    25741 cgtttccgtg atatgtgcgg tactgcagcg tatgccggca agggttgcaa atggcggagg
    25801 tgtgcaggtt gactgttggt cggattcctc caccacacgg agtgaataac cgttttcagc
    25861 gacgttatta atcagttcag tgagattaac accatcgacg tcaacgacaa tgcgccccat
    25921 gttcagtgcc tgcacgttaa cgctgtcagc ttccagcgtc agggccagtt tcatcgtttc
    25981 gcctcctgat acttacccag ggtaatgtta tttaccgttc ggtaattgtc gcgggtcatc
    26041 aggccagtcg cccggcgagc ccggaggata tcgatgctgt ttattaactg agagcgggta
    26101 caggcactga attccggctg gtcggtacgc accagtgcgt atttttcaac aagaaagttc
    26161 actgcatcgc acagtgaaat gccagcttca atatgctgct caatcacacg ttcatcggcg
    26221 aacggtgtgt cattcagtgt aaggccgtaa tgctggccca gtagtcggga gagcagagtc
    26281 tgccagatgg tgacgggaga cgggcagctc gatgcctccc gtacgtgtgt atcgggtaat
    26341 gttttcatac tgaaggtttt cctgatatgc agagatgaaa aatggaaaag tggcgtggtg
    26401 aaagcaccag gccggagcag aaggttattc cggtggttta ctttttcgtt tcgggcgtcg
    26461 gataaacagc cagataaacg taaccaccgc taccgagagt gtcagctttg caggtcagac
    26521 cttttgcata cagcgtgacg gtatgctgat ggcggggatt cagttcaccg ctggtgagca
    26581 tgagttccag ttgtttcatc agcagcggaa aggcctggtc caggtggtac gcatctgcat
    26641 cgctgaaccg gcctctgata ccggcacggt cggcaaggta atgcaaccgg ttaccctcct
    26701 gcaccagacg ggcgccgaaa cagggcgtca ctgtgcaggg tagtccccac cagggatggt
    26761 cgtcattgtc atcgggatgc gttgtcccgg aaagtgtgtc tgacacgata aaatcctcac
    26821 agaaaatcgg tgaatgatag tttaacgatg acgggtcagc cggcgcgcag accggcttcg
    26881 gtaaaggtgt attcgttgat gagcaggacc tcatccacag cggcgtctga gctcagccag
    26941 tcgtactcgt tttccgggtg acggaacatc agttcagggt gacgctcatc agccggacat
    27001 acggaccaaa actgtcctta cggcgttctg caaacacggc cagcacaccg ggaatatcct
    27061 gcacttcacg accggtatac gcttcagcac tgccgtgcca gcggtatttg ccggtacaga
    27121 acggaaaaag acgggatgcc ggatgctgac ggtgaatacg catggcttcg ccacgggtga
    27181 tgattttcat agtgggatac ctctgaagac agaagataaa agtgaaaaca ggtgtgatgt
    27241 ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggc tgccaccgac
    27301 tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca gacgttccgt
    27361 gataaggcgg tctgccttgc tgggtgtgac ttcaccggac gggtgattgt gtgccagcac
    27421 cacagcagcg gcattgtgat acagggcgcg tttaatcact tcacggggat ggacttccgt
    27481 gcggttgatg gtgccggtga agagggtttc accggcaatc aactgattct ggttgttcag
    27541 atacagcacc cggaactctt cacgctccag tcccgccatg ttcagaatca gccattcccg
    27601 tgccgcacgg ggggaggtga aggccacacc gggttcatga agatggcggt ccagggtttt
    27661 cagagcccgc agaatgagac tgcgctcgcc gggcgtcatt tctccgggca gaaaggaaag
    27721 ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc tgcattccgg
    27781 atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat aatactccgt
    27841 acgacaggcg tgatgactgt acgtcatcag acaggcagca atgccggcgg cttccgggct
    27901 catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgc catcatcgtc
    27961 atccggttcg ggcgctataa atgcgccacc gttgttcagt gtgtacagat tccagatacc
    28021 accgcagtag tcttcgcaaa gacggtccat ccagccgaag atacggggct ccagggtcac
    28081 ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt catcagggac
    28141 tatggtggca accagctgag gctggtcatt ccctgatgca gcggttacgg aaacagaaga
    28201 tgtggtggaa ttatgtgaaa tggttttcat gaaatgactc cttaaaggac aaataaatgc
    28261 gtggttttca gggtaatggc tgaatcagcg caggctctcc agcagcgttt ccgccatcac
    28321 ccacaatgcg cggttgagct taatgtcagt gtcaatgctg tgaatggcac gggtatggat
    28381 acgttttcct ttagcgctgc gacctgaaat tccgcctttc agcatattct cctgaatagt
    28441 ctgataagca ctccacaggt ccttaccgta atcctcccgg cgtcgtggcg tcagaatgtc
    28501 ggcggtggtg acgggctgat gttcgtcacc ataacggtaa gtcagtgccg cctgtgccag
    28561 cgcctggcgt gccggtggcg gcagaatcag cgactgcatg gcatcacgct tttcctcaat
    28621 ccggtcaaaa acccccacca cctcgtaagc cccttcaatg actctgtcca ccacatttcc
    28681 ccggtgcgga acacgcactt cccccagaga ctgaccacag acgcatccgt tctggcagac
    28741 gaacctgaag taacccggca gcatctggta gctggaggta ccgtcatgag agttgagcag
    28801 aataatttca gggacatgtt ctccgtttat ctctccggcc cgccgcagac gcagcatgtg
    28861 ttttgtgtat ccccggcggc ccgggtcgcg cacacgggtc tggcaggcga agaatggctg
    28921 aaagccttcc cgctgcaggc tttccagtac ggtgatggtg gggatgtacg tatagttctg
    28981 gcttcgggac gtgtgtttat cttccccgaa aatccccggc acatagtgca tcagttcttc
    29041 gtgtgtcagc ggacggtcac ggcgtatctg gttcgcataa ccaaaacgac tggctagtcg
    29101 cataatttgc tccttatcgg tggttaagat ttactggtgt aataaatgaa aaagccacgt
    29161 ctcccggaga agacgcggcc tgatggatga aatgaagacg tttattgcct gagaattccc
    29221 tcactggcga gatacgaatt tagctgtttt ccggcatcag gttcaaaatt ccagcaccgc
    29281 cagcgtagct gcccttcggc attgcggata accagccgga aatgcgttcc ctggtcatct
    29341 tcaataagca cattgcggta agctgttgtg acggcaaccg cctgttcgcg tgagaaaggg
    29401 ccttcgggca ataaaacagg gtggcagtcc gacataccgg ctattttccg ataaattcaa
    29461 cgctggcgcc ggtggcatta ccgtcagcac caaccgtgac ggtcactttg tcgtacgcac
    29521 ggtcttcagg attgaatact tcaaactcaa ccttattgcc ttttacttcg taagagccaa
    29581 aatcagcggt ttctgttgtt tcctcgtcat attcccgggc gtatgcctgc cgggcagcct
    29641 gcaggacgga atctttatta atagtggtcg tctgtttcgg ggaaaaaccg gcaagtgatg
    29701 tgacaaattt cggacagacc ttcagcatat caccgcgggt gaattccatt cgtataccaa
    29761 atgcctgtaa aacggacaca tcacgctctg ctatggagtg aacggctttt ttgaccattt
    29821 ccggcgcctg tccgatttcc acctcaacag ccctgaatcc cgtctgctga ttacacattt
    29881 gtgatgtggg gatcaggtta ccgttaatac tgatacggct gcccaggagc gtctgtacgc
    29941 cattcgggca tccctgcatc aggccggtaa taccagtcgt gccatttttc agcattacca
    30001 gcgcctgacc atcttccgtc atggtctgac accagccgtt tttgacagcc cagttgccgg
    30061 cggaggcgga gaatgatgcc acagtgaaca gcaggggaaa aagagctctg gtattcatga
    30121 ctgacctctg aaatgaacgt acggtgttat tgataatgat ggaaggtatg cagtaactgt
    30181 tcagggaatg acgtagccgt atatttagtt ttctgttcaa catattgtct ccatacctgt
    30241 cgggacggct ttggcaggcc ggcgatcaca ggttaaaggt gttgataagt ttatggatga
    30301 aagagaaatg tggcagggcg gagataaaac gctcttcagc gaccagactg tggatgtggc
    30361 ctctccgttc aaaccgttct gatgtcaggc tgactgattt gatatctgcc agaggaaaga
    30421 cctgcagttt tcccttctgt aacgcgatca gataccagct accgctgtaa taaatgagcc
    30481 ggcagggctc cagtgacgga taccatcgct cagtcatcag agagatgtgg atacactgtc
    30541 ttattgccag gatgagttgc gagaaacact cggggaacgt tgcagtgata tccggcgccg
    30601 gtagccagat aaggcaggga gagggctcct ggttgtcggt aagacaggtt atcagtcgtc
    30661 cgttccggag cggaagtatc cgtgcgatcc cggtattctg tataaaagaa agcatttcag
    30721 ggatcgcacc tgggctgctc tgtcgtctga ggctgtacct gccatttctg tactctaaat
    30781 ccaggtgaac cagacgctga tgaaaatcgc gctgtaaagt acgttctgta acgccaaatt
    30841 catctgacag tgtttttagt gacagagatt ctccggccat cagtcggctg ataatgagtg
    30901 ataagcgaac cgccaggcga tcatgccggg aacgattatc agacatagat gtgcactccg
    30961 taagaaaaca tcttttattt catgatgtta ttatgcatca tgatgttgaa aggatgtgtc
    31021 gcatccggat aatcaggaat aaaatgcagc agaaaaaata ttcttttaat tatctgattt
    31081 ttaagcatga tttcataaaa agaagaatgc tcgttaatcc agcgacacat cctgtcggcg
    31141 tgagtgtgtc agaacagacg ctcccacagt gtgatcagca ggtggataat gcggtcccgg
    31201 gctttacgca gaagctgtaa catccaggcc ggaaaactga aacgggcaac ggcgacgtca
    31261 aaacttttcc cgatggtttc accaaaagtc tgttgtgcgt gtttctgggc ctgttcagag
    31321 cggttttctc cccggatatg tgaatacacc gcgctggttg cctgtggtgg cagtgcgtgg
    31381 atcatcagcg acacaaacgc cggaaggttc cagccgacag gtgcagcaac ggagagtacc
    31441 ggaaatgatg aagggaacag ggttgccacc cgggctgtca ccgtcgccag tgagagttcc
    31501 tgctgacggg acgggcattt ttctgtggca ttccattctt cagcaggaaa cacgcgatcg
    31561 gcatggctga gaacaaacag aaaacgcgag ggatctgcgc cttcattcag taaaaactga
    31621 tgcatggtaa tgtcggcggc atacgcgcgt tcatctgccc gcaggatcca gataatcaaa
    31681 tccacttccg gcagtaactg acggtaaagc gcccggtatt cctgatcatg ttgcggcgtc
    31741 tctccaatgc cgggcagatc gaccagcgtc attctgcgtt caccgatctg cagggtaaga
    31801 cgatgagcct ggcgggtgca gccgttcagg ggatgcgtgg cacagatatg ggactgaaaa
    31861 atagcattac acaggctact tttgccggtg ccggatttac ccataatgcc aatcacgggt
    31921 tcgtaatgaa taacgctgtg gagatgttca agaattttct gacgaatttc ttcaggaaga
    31981 aacgtcagat atgatttcac ttgtgcggag agggaagaat ggctgaatga cataccggta
    32041 tcctgttgat ttcactgaac cggatcacac taatccggtt tatcattcag gtaatatggg
    32101 cgtgaaaaat gatggaatgt ctgaattacg gacattattg ttaaagtgta atggagtatg
    32161 gcgtcagttt ttcagaacgg gaactaatga catactgctg tttcaggaga tgagaggatg
    32221 accggacgta aaaacaaatg tgtcagaaaa aattgtaaca atcaggtttt ctcagtaaaa
    32281 atggcagtgg agaaaagact gtgcagaaaa cattatgaga ggttttgtga gctcaaaaaa
    32341 agaagagagc aaaatcctgt tcacagatta aatgaagcat tgccaagaaa ggtagaaggc
    32401 ccaaaaagaa agaggaaatt agtaaaagaa agagaaatca ttaacagcag gacgaaacat
    32461 aaacaggagc agggaccatc tctggctgaa cttaataaaa aagttcgtga gatactgaaa
    32521 accccggcat ggagaacgct ggaaacgaga attcgaagaa acggtatcac atttaattca
    32581 aaagcagagt tagatgcgct ttctgaactt tatgcaattt attcatgggt ttcatattgc
    32641 aaacggtgcg ataattattc tgtgtggttt ttgaattgtc cggatgtaaa tataaatctt
    32701 catgtgtgcg aatgccggtc ttctgaaagc gattgttatc tgccatcccg ttttattatt
    32761 cttccagctc cattaaggca aaaagtaaaa agaaaatact ctgctaattc gtctggggta
    32821 tcaggacata atgattcaaa aagtctttat aagacagttt tgtctgtttg tggtgggaaa
    32881 agagaaaccg aattgttctt ttattcactg ccagtaaaga ctaatcatca tccttactgg
    32941 attaaaagtg gtgtggttaa aagatttcct cctgtttttg gtttgttgtt ttatgaacag
    33001 gaaagattaa caggaggtgt ttttcgttat gtgttgataa ggttttataa tacttttaag
    33061 gagaatttgt tttttgttga gattgtctcg tgctgttttc ttgttgagct ttttcatgat
    33121 attgattcgt tttttataaa tcaacgatta agtaa
//
LOCUS       DAPGRN010000046        32645 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPGRN010000046 DAPGRN010000000
VERSION     DAPGRN010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.046"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(129..3278)
                     /locus_tag="RHW33_002331"
     CDS             complement(129..3278)
                     /locus_tag="RHW33_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV1645827.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHIVDHH"
     gene            complement(3301..4494)
                     /gene="acrA"
                     /locus_tag="RHW33_002332"
     CDS             complement(3301..4494)
                     /gene="acrA"
                     /locus_tag="RHW33_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV1645828.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQANALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4636..5283
                     /gene="acrR"
                     /locus_tag="RHW33_002333"
     CDS             4636..5283
                     /gene="acrR"
                     /locus_tag="RHW33_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV1645829.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5411..8773
                     /gene="mscK"
                     /locus_tag="RHW33_002334"
     CDS             5411..8773
                     /gene="mscK"
                     /locus_tag="RHW33_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV1645830.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATSALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8985..9146)
                     /gene="rsmS"
                     /locus_tag="RHW33_002335"
     CDS             complement(8985..9146)
                     /gene="rsmS"
                     /locus_tag="RHW33_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV1645831.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9160..9687)
                     /gene="priC"
                     /locus_tag="RHW33_002336"
     CDS             complement(9160..9687)
                     /gene="priC"
                     /locus_tag="RHW33_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV1645832.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLHEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9757..10134
                     /locus_tag="RHW33_002337"
     CDS             9757..10134
                     /locus_tag="RHW33_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV1645833.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10287..10838
                     /gene="apt"
                     /locus_tag="RHW33_002338"
     CDS             10287..10838
                     /gene="apt"
                     /locus_tag="RHW33_002338"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV1645834.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10967..12898
                     /gene="dnaX"
                     /locus_tag="RHW33_002339"
     CDS             10967..12898
                     /gene="dnaX"
                     /locus_tag="RHW33_002339"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV1645835.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12231..12295
                     /gene="dnaX"
                     /locus_tag="RHW33_002339"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12951..13280
                     /locus_tag="RHW33_002340"
     CDS             12951..13280
                     /locus_tag="RHW33_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV1645836.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13280..13885
                     /gene="recR"
                     /locus_tag="RHW33_002341"
     CDS             13280..13885
                     /gene="recR"
                     /locus_tag="RHW33_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV1645837.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13995..15869
                     /gene="htpG"
                     /locus_tag="RHW33_002342"
     CDS             13995..15869
                     /gene="htpG"
                     /locus_tag="RHW33_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV1645838.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16050..16694
                     /gene="adk"
                     /locus_tag="RHW33_002343"
     CDS             16050..16694
                     /gene="adk"
                     /locus_tag="RHW33_002343"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV1645839.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16826..17788
                     /gene="hemH"
                     /locus_tag="RHW33_002344"
     CDS             16826..17788
                     /gene="hemH"
                     /locus_tag="RHW33_002344"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV1645840.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17785..18744)
                     /gene="aes"
                     /locus_tag="RHW33_002345"
     CDS             complement(17785..18744)
                     /gene="aes"
                     /locus_tag="RHW33_002345"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV1645841.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18896..20200
                     /gene="gsk"
                     /locus_tag="RHW33_002346"
     CDS             18896..20200
                     /gene="gsk"
                     /locus_tag="RHW33_002346"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV1645842.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20330..22006)
                     /locus_tag="RHW33_002347"
     CDS             complement(20330..22006)
                     /locus_tag="RHW33_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV1645843.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALDLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22244..23464)
                     /gene="fsr"
                     /locus_tag="RHW33_002348"
     CDS             complement(22244..23464)
                     /gene="fsr"
                     /locus_tag="RHW33_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV1645844.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23682..25334
                     /gene="ushA"
                     /locus_tag="RHW33_002349"
     CDS             23682..25334
                     /gene="ushA"
                     /locus_tag="RHW33_002349"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV1645845.1"
                     /translation="MKLLQRGVALALLTTFTLASEAALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKLGNPENFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGDHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25371..25850)
                     /gene="ybaK"
                     /locus_tag="RHW33_002350"
     CDS             complement(25371..25850)
                     /gene="ybaK"
                     /locus_tag="RHW33_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV1645846.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26054..26848)
                     /locus_tag="RHW33_002351"
     CDS             complement(26054..26848)
                     /locus_tag="RHW33_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV1645847.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPARLLKKLKNADALIVEADVSTNDMPFANLPACEALEERISEEQLQKLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26986..27327
                     /locus_tag="RHW33_002352"
     CDS             26986..27327
                     /locus_tag="RHW33_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV1645848.1"
                     /translation="MKQATRKPTTPGYILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27542..30046)
                     /gene="copA"
                     /locus_tag="RHW33_002353"
     CDS             complement(27542..30046)
                     /gene="copA"
                     /locus_tag="RHW33_002353"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV1645849.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALF
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADFDEAQALRLAAALEQGSSHPLAR
                     AILDKASDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30308..31240
                     /gene="glsA"
                     /locus_tag="RHW33_002354"
     CDS             30308..31240
                     /gene="glsA"
                     /locus_tag="RHW33_002354"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV1645850.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGELVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEDGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31243..32535
                     /locus_tag="RHW33_002355"
     CDS             31243..32535
                     /locus_tag="RHW33_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV1645851.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
BASE COUNT         7923 a         8642 c         8573 g         7507 t
ORIGIN      
        1 tgaattttat ccgtggttaa tactggtttt cgtatgagat cctgagttgg tggttcaatt
       61 actccttaat gttcgtaggt tatgcataaa aaaggccgct tacgcggcct tagtgattac
      121 acgttgtatc aatgatgatc gacaatatgg ctgtgctcga tatcttcatt cttgcggcta
      181 aagcggcggc gaaccaccac aaagaatacc ggaacgaaga agattgccag taccgttgcg
      241 gtcaccatcc cgcccattac accggtacct actgcgttct gcgcgccgga accagcacca
      301 gtactgataa ccagcggcat aacgccgagg ataaacgcca gcgaggtcat caggatcgga
      361 cgtaaacgca tccgcaccgc atcaagcgtc gcttcaatca gacctttacc ttctttatcc
      421 atcaagtctt tggcgaattc gacgataagt atcgcgttct tcgccgacaa cccaatggtt
      481 gtgagcaggc ctacctggaa gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc
      541 aacgcaccga taacccccag cggaacgacc agcataacgg agaacggaat cgaccagctc
      601 tcgtacagcg ccgccagaca caggaatacg acaatcaacg aaatcgcgta cagtgaaggt
      661 gcctggttgc cggagaggcg ttcctgatag gacatccccg tccagtcata gccaacaccg
      721 gtaggcagtt tgctcgccag ttgttccatc agctccattg cttcaccggt acttttaccc
      781 ggtgccgcct ggcctaagat ttccatggat ggcaggccgt tgtaacgttc cagacgcggc
      841 gaaccgtact cccaacgaga agaggagaac gccgagaatg gcaccatctg accatcagca
      901 gcacgaacat accagtcgcc gatatcatcc ggcagcatac ggtatttcgc ttctgacatg
      961 acgtaaactt tcttcacacg accgcggtcg ataaagtcgt tcacatagct gccgccccat
     1021 gcagcgccca gagtggtgtt aatgtcgttg atagaaacac ccagcgcctg cgctttttcc
     1081 tggtcgatat caatcttaaa ctgcggggta tcttccagac cgtttggacg tacgctggtc
     1141 aacatatcag ggtgcttcgc tgcttctgca agcagctggt tacgcgcctg agtcagtttt
     1201 tcgtgaccaa ggccagcctg gtcaatcagc tcaaagtcaa agccggttgc agtacccagt
     1261 tccacgattg cgggcaggtt aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg
     1321 cgtgttgcac gcatggtaat cgcttcaact ttgttttctt cgcccggacg atcggcccag
     1381 tccttcaagg aaacgaacgc aataccggta ttctgaccac gtcccgcaaa gccgaagccg
     1441 ttaacggcga acaccgactc aacgttgttc ttttctttgg tcagatagta atgcgttacc
     1501 tcattgagca ctttctgtgt acgttcctgc gttgcacctg ctggcagctg aaccatggtc
     1561 ataaatacac cctggtcctc atctggcaag aaggagcttg gcagacgcac gaacagatag
     1621 gccatgccga ccacgatgat cagatacagc accagataac gccccgtact gcgcaaaata
     1681 ccgcctacgc tgtcggtgta gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg
     1741 aagaagcctt ttttaccttc cccgtgatcg cctttggcaa tcggtttcag catggtggca
     1801 caaagagctg gagtcaggat caacgccacc agtaccgaca gcgccattgc tgaaacaatg
     1861 gtaatagaga actgacgata gatagcacca gtagaaccgc caaagaaggc catcggtacg
     1921 aataccgccg acagtaccat cgcgataccg accagagcgc cctgaatctg ccccatcgac
     1981 ttacgggtgg cttcttttgg cggcaaacct tcttccgcca tcacacgctc aacgttttct
     2041 accacaacga tagcgtcatc caccaacagg ccgatggcga gcaccatccc gaacattgtt
     2101 agcgtgttta tcgagaagcc aaaggcggca aggacggcaa aggtcccgag caataccacc
     2161 ggtacggcaa tggtcggaat caacgtcgcg cggaagttct gcaggaacag atacataacc
     2221 aggaacacga ggatgatcgc ttcgaccagc gttttaacca cttcgtgaat agagattttc
     2281 acgaacggcg tggtgtcgta cgggtaaaca attttcagac ccgacgggaa gaacggttcc
     2341 atcttcgcca gttcagcacg gattgccgca gcggtatcca gcgcgtttgc accggtcgcc
     2401 agcttgatcc ccagaccgga agccggttgg ccgttaaact ctgcgatgat gtcgtagttc
     2461 tcaccaccca gctcaatctt cgccacatca cgcagcagca cgcgggaacc atcctgattc
     2521 actttcagca ggattttgcc aaactcttca gtagaggtca gacgcgtctg agcaataata
     2581 gaggcgttaa gctgttgccc tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc
     2641 tgggcgttct gcgctttgat ggctgtaata acatcaaccg gtgttagctg gaatttgttc
     2701 agttcattcg ggttcatcca gatacgcatc gcgtactgtg aaccgaacaa ctgaacgtca
     2761 cccacgcccg acgtacggct gatggcatct ttcatattcg ccgccacgta gtcggagata
     2821 tcctcctgcg tcatggtgcc atcggtgttg ataacgccga caaccatcag gaagctgctg
     2881 gatgatttct caacgctcac cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc
     2941 agctgcagtt tgttctgcac ctgaacctgc gcgatatccg catcagtacc agactcaaag
     3001 gtcagggtga tctgcacggt acccgtggag tcactgttag aggacatgta catcaggtta
     3061 tcgataccgt tcatattctg ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca
     3121 tcagcgccgg ggtaggaggc ggagatcgtt actgccggcg gtgcaatcgt aggatattgc
     3181 gccaccggca gtttgaggat cgccagcccc cctgccaaca tgatgataat ggcgatcacc
     3241 cacgcaaaaa tcgggcgatc gataaagaaa ttaggcatgt cttaacggct cctgtttaag
     3301 ttaagacttg gactgttcag gctgagcacc gcttgcggct tgctggttat tatcagcggt
     3361 aacttcttgt gcttttacct ggacaccagg acgcactttc tgcagcccac ttattactac
     3421 gcgatcgcct gctttcagac cttctgtcac cagccactta tcgccaatag cctggcttgc
     3481 aacgatcgga cgggtttcca ctttgtcatc cgcgccaact accagtacgg tggcatcgcc
     3541 acgcggcgta cgggttacgc cctgttgcgg gactaaaata gcgtttggat taagcccttc
     3601 ttccagacgt gcgcgcacga acatacccgg cagcagagtg tgatccgggt tcgggaagat
     3661 agcgcgtaga gtgatagacc cagtggtctg atcaacggta acgtcagaga attccagcgt
     3721 accgtcctgc gggaacttaa tgccgtcact ggtgatcagt gacactttgg ctttgccgtt
     3781 ctcttgtttc agcgtgccat tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga
     3841 ctgggtcaca tcaacgtaga tcggatcaag ttgctgcacg gttgccagcg cattcgcctg
     3901 accgttctgt accaatgcgc cttccgtcac gttcgactta ccaatgcgac cgctaatcgg
     3961 agaggtgact ttggtgtaag ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc
     4021 agttaccgca gcattcgcct gttgcgcatc agccagagcc tgatcgtact cttgcttact
     4081 gatgtactga gtaccgagca gtttctgata acgattcacc gtcaattgcg cgatattggc
     4141 tgcagcctgg gctttcgcca gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc
     4201 aggatcaatc tgatagagag agacacctgc ttcgatgtcg ctaccttctt tgaaattacg
     4261 cttcaggata atcccgctaa cttgaggacg aacttctgcg atccggtagg cactggtgcg
     4321 acccggaagc tcggttgtga tctgcagagg ttcagttttg actgttacta cgccaacggc
     4381 gggcatctgc tggccacctt gttgggcctg tttgtcgtca catcctgtta gggctaagct
     4441 gcctgagagc atcagaacga ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa
     4501 acctcgagtg tccgatttca aattggtcaa tggtcaaaag ttaataaacc cattgctgcg
     4561 tttatattat cgtcgtgcta tggtacatac attcacaaat gtatgtaaat ctaacgcctg
     4621 taaattcacg aacatatggc acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac
     4681 atcctcgatg tggctctacg tcttttctca cagcaggggg tatcatccac ctcgctgggc
     4741 gagattgcaa aagcagctgg cgttacgcgc ggtgcaatct actggcattt taaagacaag
     4801 tcggatttgt tcagtgagat ctgggaactg tcagaatcca atattggtga actagagctt
     4861 gagtatcagg caaaattccc tggcgatcca ctctcagtat taagagagat attaattcat
     4921 gttcttgaat ccacggtgac agaagaacgg cgtcgattat tgatggagat tatattccac
     4981 aaatgcgaat ttgtcggaga aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg
     5041 gaaagttatg accgtataga acaaacgtta aaacattgta ttgaagcgaa aatgttgcct
     5101 gcggatttaa tgacgcgtcg cgcagcaatt attatgcgcg gctatatttc cggcctgatg
     5161 gaaaactggc tctttgcccc gcaatctttt gatcttaaaa aagaagcccg cgattacgtt
     5221 gccatcttac tggagatgta tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa
     5281 taaccctgaa tctgactcca ggatttttcc tggacatttt cgtcgttgct attctggttc
     5341 actgcgtcgt gatattcttg cggtttgact ttttcaggtc gttcttcagg ttcagaaacc
     5401 ttcattcatc atgactatgt tccagtatta caaacgatca cggcattttg ttttttcagc
     5461 atttattgct tttgtttttg tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc
     5521 gaatggtgat ctgccgacaa aagcggacct gcaggcgcaa cttgactcac taaataaaca
     5581 aaaagatctt tctgctcagg acaaactggt gcagcaggat ctgacagata cattagccac
     5641 cctcgataaa atcgatcgcg taaaagaaga gaccgttcag ctacggcaaa aagtcgctga
     5701 agcgccggaa aaaatgcgcc aggcgacctc ggcgttaaca gcacttagcg atgtcgataa
     5761 cgacgaagaa acgcgcaaaa ttctgagcac gctttcgttg cgccagctgg aaactcgcgt
     5821 tgcccaggcg ctggacgatt tgcaaaacgc acaaaacgat ctagcgtctt ataacagcca
     5881 gctggtttcg ttacagacgc agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca
     5941 gcagctgcaa caaattcgca gtcgtctgga tgggactgat gtcggcgaga ctgccttacg
     6001 tcccagccag aaagtgttaa tgcaggccca gcaggctttg ctgaatgcgg agattgacca
     6061 gcagcgtaaa agcctggaag ggaataccgt cttgcaggat accttgcaaa agcaacgtga
     6121 ttacgtgacg gcgaacagcg cccgtctgga gcaccagttg cagctgttgc aagaagcggt
     6181 aaacagcaag cgcctgactt taaccgaaaa aacggcgcag gaagccgtct ccccggatga
     6241 agccgcgcgt attcaggcta atccgctggt gaagcaggaa ctggaaatta accagcagtt
     6301 aagtcagcgt ctgattaccg cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa
     6361 agtcaaaaac tggctggagc gggcgctgca atcggaacgc aatattaaag agcagattgc
     6421 cgtcctgaag ggcagcctgc tgttgtctcg tatcctttac caacaacaac aaacgctgcc
     6481 ctcggcggat gaactggaaa acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt
     6541 tgaagttaac cagcagcgtg atgcactctt ccagagcgat gcgttcgtca acaaactgga
     6601 agaaggtcac accaacgaag tcaacagcga agttcacgat gcgttattgc aagtggttga
     6661 tatgcgtcga gaattgctgg atcaactcaa caaacagttg ggtaaccaac tgatgatggc
     6721 cattaacctg caaatcaacc agcagcagtt aatgagtgtg tcgaaaaacc tgaaatccat
     6781 cctgactcag caaatctttt gggtgaacag taaccgtcca atggactggg actggatcaa
     6841 agcgttcccg caaagcctga aagatgaatt taagtcgatg aaaattacgg tgaactggga
     6901 aaaagcctgg cccgccgttt ttatcgcttt cctcgctggt ttgccgctgc tgttgattgc
     6961 cgggctgatc cactggcgtc tggggtggct gaaagcgtat caacaaaaac tggcttccgc
     7021 tgtgggttcc ctgcgtaacg acagccagct caacacacca aaagcgatcc ttatcgacct
     7081 gatccgtgca ctgccggtgt gcctgattat tctcgcggtt ggcctgattc tgttgaccat
     7141 gcagctcaac atcagcgaac tgctatggtc gttcagtaaa aaactggcga ttttctggct
     7201 ggtgtttggc ctgtgctgga aggtactgga gaaaaatggc gttgccgtgc gtcacttcgg
     7261 catgccggag cagcagacca gccactggcg tcggcaaatt gtccgcatca gtctcgcgtt
     7321 gctgcctatc catttctggt ctgtggtggc agaactttcc ccgctgcatc tgatggatga
     7381 tgtgctgggg caggcgatga ttttcttcaa cctgctgctg attgccttcc tggtatggcc
     7441 aatgtgccgc gaaagctggc gtgataaaga gtcgcacacc atgcgactgg tcaccattac
     7501 cgtgctgtcg attatcccga ttgcattgat ggtgctgact gccacgggtt atttctacac
     7561 cacgctgcgc ctctccgggc gttggattga aaccgtttat ctggtgatca tctggaactt
     7621 gctctaccag acggtattgc gcggcttaag cgtagcggcg cggcgtatcg cctggcgtcg
     7681 tgcgctggcg cgtcggcaga atctggtgaa agagggcgca gaaggtgccg aaccgccgga
     7741 agaacccacc attgcactgg aacaggttaa ccagcagacg ctgcgtatta ccatgttgct
     7801 gatgtttgcg ctgttcggtg tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt
     7861 cagctatctc gacagcatta cgctgtggca ttacaacggt actgaagctg gtgctgcggt
     7921 ggtgaaaaac gtcaccatgg gtagtctgtt gtttgcgatt atcgcctcaa tggtggcctg
     7981 ggcgttgatt cgcaacctgc ctggtttgct ggaagtgctg gtgctctcgc gactgaatat
     8041 gcgccagggc gcgtcgtatg ccattactac catccttaac tacatcatta ttgctgttgg
     8101 tgcgatgacg gtgttcggat cgctgggcgt ctcttgggat aaactccagt ggctggcagc
     8161 agcattatcc gtaggtcttg gttttggttt acaagaaatt ttcggtaact tcgtctccgg
     8221 tttgatcatt ctattcgaac gtccggtgcg tattggcgat acggtaacca ttggtagctt
     8281 ctcggggacg gtaagtaaga tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa
     8341 agaagtgatc atcccgaaca aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac
     8401 tgacactact acgcgtctgg tgatccgtct cggcgtggcc tatggctccg atctggaaaa
     8461 agtgcgtaaa gtgttactga aggcggcgac tgagcaccca agggtgatgc acgaaccaat
     8521 gccggaagtc ttctttacgg catttggtgc gagcacgttg gatcatgagc tgcgtctgta
     8581 tgtgcgtgaa ctgcgtgacc gtagccgtac tgtcgatgag ctgaaccgta ctatcgatca
     8641 gctgtgccgt gaaaacgaca tcaacattgc ctttaaccag cttgaagtgc atctgcacaa
     8701 cgagaagggc gatgaggtga cggaagtaaa acgcgactac aaaggcgacg atccgacgcc
     8761 agcggtaggg taataacgaa agggggcgac attttgttgc cccgagattg ctaacaaagt
     8821 gcgcgttgtt catgccggat gcggcgtgac cgccttatcc ggcctacgaa accgcaaaaa
     8881 ttcaatatat tgcaggagcg gtgtaggcct gataagcgta gcgcatcagg cagttttgcg
     8941 tttgtccgca accttagggg acatttagcc accccattta tttctcactt ttccgcctca
     9001 tcatcgcgcg ttaatttctt ttcataatcg cgttttacaa tatccagcgc gcgcagcacg
     9061 gtactggcag ggatctgatt ttcctccagc agcacaatca aatcgacagc cagtttgaca
     9121 tcgtcagggg cattttccag tgacatactc tctccattgc tagcgggtta aacgcgctaa
     9181 cctgttttcg attttttcca gcgcatggcg acaccgcgcc aggcgcgctt cataggcttc
     9241 cacttcacga tgcagcgttt gctgttccac gagatcggtc acccgcgcca ggcgggctct
     9301 gcgttcggca accatctcat gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg
     9361 ctgccagcgg gcaattttcg gtggcgcact gtcccactcg cgcaatgacc aggcggaggc
     9421 ttcacgcgcg atggcttcca gttgtgccgc cagatgttcc gccagccagg ccacttgcgg
     9481 cagctgttgc tgctcaactg catgacgaag cgcagccaga ttatcgcccg cctcgtcgag
     9541 acaagcctgt agtgttgtcg cacgagtctg aaaaagatgc ctgtcgaaac gagcacttag
     9601 cgtggcgaac tgtgacaccg gggcacaacg ctgacgcagc gtagcgagct gtccttccag
     9661 tttttccagc agcagggcgg ttttcacgat aagacctcaa tgaaaatgat aattgttatg
     9721 ctaaagtagc cactctgtaa gctgacaaca ctcaatatgc aacgaatcat tttaatcatc
     9781 attggctggc tggcggtagt gctgggtacg ctgggcgtgg tattaccggt attaccgacg
     9841 acgccgttta tcctgctggc ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc
     9901 tggttgctgt accgctcatg gtttggcagc tatctacgtt tctggcagaa acatcatgcg
     9961 atgccgcgcg gcgtcaaacc gcgggcgatt ttgcttattt tgctcacgtt tgccatttct
    10021 ctgtggttcg tccagatgcc atgggtgcgc atcatgttgc tggtaattct cgcctgtttg
    10081 cttttctata tgtggcgaat tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca
    10141 acaatcgcag ttgcaattat tgcgtacagc cagtacattc tggcgttttc gagcacaggc
    10201 gcaggcggtc aaaggttaaa caactgttac ttttgatacg tttaaaacgc gccgtgagta
    10261 ccaccgtaac aagcaggcat acacttatga ccgcgactgc acagcagctt gagtatctca
    10321 aaaatagcat caaaagcatt caggactacc caaaacccgg cattcttttc cgcgatgtca
    10381 ccagcttact ggaagacccg aaagcttacg ctctcagcat cgacttgctg gttgagcgtt
    10441 acaaaaatgc gggcattacc aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg
    10501 ctccggtagc tctgggtctg ggcgttggct ttgtgccggt ccgtaaaccg ggcaaactgc
    10561 cgcgtgaaac catcagtgaa acttacgacc tggaatacgg caccgatcag ctggagatcc
    10621 acgttgatgc catcaaaccg ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg
    10681 gcggcactat cgaagcgacc gttaaactga tccgtcgtct gggtggtgaa gtggctgacg
    10741 ctgcgttcat tatcaacctg ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca
    10801 ttaccagcta cagccttgtc ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc
    10861 cacgctacgg acagcacaag atgtgcattc agcctcgccg ttctgacggg gctgtgttag
    10921 cattacccct tcgtgaatcc accttccagc gtttcagagc ctgccaatga gttatcaggt
    10981 cttagcccga aaatggcgcc cacaaacctt tgctgacgtc gtcggccagg aacatgtgct
    11041 gaccgcactg gcgaacggct tgtcgttagg gcgtattcat catgcttatc ttttttccgg
    11101 tacccgtggc gtcggaaaaa cctctatcgc ccgactgctg gcgaaggggc taaactgcga
    11161 aaccggcatt accgcgacgc cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg
    11221 gcgctttgtc gatctgattg aaatcgacgc cgcctcgcgc accaaagttg aagatacccg
    11281 cgacctgctg gataacgtcc agtacgctcc ggcgcgtggt cgtttcaaag tttatctcat
    11341 tgacgaagtg catatgctgt cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga
    11401 gccgccggag cacgttaagt ttctgctggc gacgaccgat ccacagaaat tgccggtgac
    11461 gattttgtca cgctgtctgc aatttcatct caaggcgctg gatgtcgagc aaattcgcca
    11521 tcagcttgag cacatcctca acgaagaaca tatcgctcac gagccgcggg cgctgcaatt
    11581 gctggcacgc gccgctgaag gcagcctgcg agatgcctta agtctgaccg accaggcgat
    11641 tgccagcggt gacggccagg tttcaaccca ggcggtcagt gcgatgctgg gtacgcttga
    11701 cgacgatcag gcgctgtcgc tggttgaagc gatggtcgag gccaacggcg agcgcgtaat
    11761 ggcgctgatt aatgaagccg ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat
    11821 gctcggcctg ttgcatcgta ttgcgatggt acaactttcg cctgctgcac ttggcaacga
    11881 catggccgcc atcgagctgc ggatgcgtga actggcgcgc accataccgc cgacggatat
    11941 tcagctttat tatcagacgc tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg
    12001 ccgcatgggc gttgagatga cgctgctgcg cgcgctggcg ttccatccgc gtatgccgct
    12061 gcctgagcca gaagtgccac gccagtcctt tgcacccgtc gcgccaacgg cagtaatgac
    12121 gccaacccag gtgccgccgc aaccgcaatc agcgccgcag caggcaccga ctgtaccgct
    12181 cccggaaacc accagccagg tgctggcggc gcgccagcag ttgcagcgcg tgcagggagc
    12241 aaccaaagca aaaaagagtg aaccggcagc cgctacccgc gcgcggccgg tgaataacgc
    12301 tgcgctggaa agactggctt cggtcacaga tcgcgttcag gcgcgtccgg tgccatcggc
    12361 gctggaaaaa gcgccagcca aaaaagaagc gtatcgctgg aaggcgacca ctccagtgat
    12421 gcagcaaaaa gaagtggtcg ccacaccgaa ggcgctgaaa aaagcgctgg aacatgaaaa
    12481 aacgccggaa ctggcggcga agctagcggc agaagccatt gagcgcgacc cgtgggcggc
    12541 acaggtgagc caactttcgc taccaaaact ggtcgaacag gtggcgttaa atgcctggaa
    12601 agaggagagc gacaacgcag tatgtctgca tttgcgctcc tctcagcggc atttgaacaa
    12661 ccgcggtgca cagcaaaaac tggctgaagc gttgagcatg ttaaaaggtt caacggttga
    12721 actgactatc gttgaagatg ataatcccgc ggtgcgtacg ccgctggagt ggcgtcaggc
    12781 gatatacgaa gaaaaacttg cgcaggcgcg cgagtccatt attgcggata ataatattca
    12841 gaccctgcgt cgattcttcg atgcggagct ggatgaagaa agtatccgcc ccatttgatc
    12901 gtaagcacag cttacgttcg tcatccttaa cgtgattgag agagaaacct atgtttggta
    12961 aaggcggtct gggtaacctg atgaagcaag cccagcagat gcaagaaaaa atgcagaaaa
    13021 tgcaggaaga gatcgcgcag ctggaagtca ccggcgaatc tggcgcaggt ctggtaaaag
    13081 tgaccatcaa cggtgcacat aactgccgtc gcgtagagat cgacccgagc ctgctggaag
    13141 acgacaaaga gatgctggaa gacctggtgg ctgcagcatt caacgacgca gcacgtcgta
    13201 ttgaagaaac gcagaaagaa aaaatggctt ctgtatcctc cggaatgcag ctgccgcctg
    13261 gctttaagat gccgttctga tgcaaaccag cccgctgtta acacagctta tggaagcact
    13321 gcgctgtctg ccgggcgttg gcccgaagtc ggcgcagcgt atggcgttca cgctgcttca
    13381 gcgcgatcgt agcggcggga tgcgtctggc gcaggcgctc acccgggcga tgtcggaaat
    13441 cggccactgc gccgattgcc gcaccttcac cgaacaggaa gtctgtaaca tctgttcgaa
    13501 tccgcgtcgt caggaaaacg gtcaaatctg cgtggtggag agtccggcgg acatttacgc
    13561 cattgagcag acggggcagt tttcaggtcg ttattttgtg ttgatgggac atctgtcacc
    13621 gctggacggc atcggtccgg atgatatcgg ccttgatcgt ctggaacagc gtctggcaga
    13681 ggaaaaaatc actgaagtga tcctcgccac caatcccacg gttgaaggtg aagctaccgc
    13741 taactacatt gccgagcttt gcgcgcaata tgacgtggaa gccagccgaa tcgctcatgg
    13801 cgtaccggtt ggcggcgagc tggaaatggt cgatggcacc acgctgtcac actcccttgc
    13861 cgggcgtcat aagattcgtt tttaagcaaa cgagagcagg atcacctgct ctcgcttgaa
    13921 attattctcc cttgtcccca tctctcccac atcctgtttt taaccttaaa atggcattat
    13981 tgaggtagac ctacatgaaa ggacaagaaa ctcgtggttt tcagtcagaa gtgaaacagc
    14041 ttctgcacct gatgatccat tctctctatt ccaataaaga aatcttcctg cgtgagctta
    14101 tctctaacgc ctccgatgcg gcggacaagc tgcgtttccg tgcgctctct aacccggacc
    14161 tgtacgaagg tgatggcgaa ctgcgcgttc gtgtctcttt cgataaagac aagcgcacgc
    14221 tgaccatctc cgataacggc gtggggatga cccgcgacga agtgattgac catctgggga
    14281 ctatcgctaa atccggcacc aaatcattcc ttgaatcgct gggttctgac caggcgaaag
    14341 acagtcagct gatcggtcag tttggtgttg gtttctactc tgcgtttatc gtggccgaca
    14401 aagtgaccgt gcgtactcgc gcggcaggcg aaaaaccaga aaacggcgtc ttctgggaat
    14461 cggctggcga aggtgaatac accgttgccg acatcaccaa agaagatcgt ggtactgaaa
    14521 tcaccctgca tctgcgtgaa ggcgaagacg agttcctcga tgactggcgc gtgcgttcca
    14581 tcatcagcaa atactccgac catatcgcgc tgccggtaga gatcgaaaaa cgcgaagaga
    14641 aagatggcga aaccgttatc tcctgggaaa aaatcaacaa agcgcaggcg ctgtggactc
    14701 gtaacaagtc ggaaatcacc gatgaagagt ataaagagtt ctacaaacac atcgcccacg
    14761 actttaacga tccgctgacc tggagccaca accgcgttga aggtaagcag gagtacacca
    14821 gcctgctgta catcccgtcc caggctccgt gggatatgtg gaaccgtgat cataaacacg
    14881 gcctgaaact gtatgttcag cgtgtgttca tcatggacga cgcagaacag ttcatgccga
    14941 actatctgcg cttcgtgcgt ggtctgattg actccagcga tctgccgctg aacgtttccc
    15001 gtgaaatcct ccaggacagc acggtaacgc gtaacctgcg caatgcgctg accaagcgtg
    15061 tgctgcaaat gctggaaaaa ctggcgaaag acgacgcgga aaaataccag accttctggc
    15121 aacagtttgg cctggtactg aaagaaggtc cggcggaaga tttcgctaac caggaagcga
    15181 tcgccaaact gctgcgtttt gcttctactc ataccgattc ttctgcgcag accgtgtctc
    15241 tggaagacta cgtttcccgc atgaaagaag ggcaggagaa aatctactac atcactgcag
    15301 acagctatgc ggcagcgaag agcagcccgc acctggaact gctgcgtaag aaaggcatcg
    15361 aagttctgct gctttccgac cgcatcgatg agtggatgat gaactatctg actgagttcg
    15421 acggtaaacc gttccagtcg gtgtctaaag ttgacgagtc gctggaaaaa ctggctgacg
    15481 aagttgatga gagcgcgaaa gaagcggaga aagcactgac tccgttcatc gaccgtgtga
    15541 aagccctgct cggcgagcgc gtgaaagatg tccgtctgac tcaccgtctg accgatacgc
    15601 cagcgatcgt ttcgaccgac gcggacgaaa tgagcactca gatggcgaaa ctgttcgctg
    15661 cggcgggcca gaaagtgcca gaagtgaaat acatcttcga actgaacccg gatcacgtac
    15721 tggtgaaacg tgcggcagat actgaagatg aagcgaagtt cagtgagtgg gtagaactgc
    15781 tgctggatca ggcgttgctg gcagaacgcg gcacgctgga agatccgaac ctgtttattc
    15841 gtcgtatgaa ccagctgctg gtttcctgat gtaatgccgg atgaccttcg tgtcatccgg
    15901 catttttctt ttcattatct gcactttccg caaattatct cgccattaac cgtttcagcc
    15961 cctggtgcct ttcttgaggc aatcgcctga tggtggtatc gtttatcgct ttttcaaaaa
    16021 attcgacaca ttttaagggg attttcgcaa tgcgtatcat tctgcttggc gctccgggcg
    16081 cggggaaagg gactcaggct cagttcatca tggagaaata tggtattccg caaatctcca
    16141 ctggcgatat gctgcgtgct gcggtcaaat ctggctccga gctgggtaaa caagcaaaag
    16201 acattatgga tgctggcaaa ctggtcaccg acgaactggt gatcgcgctg gttaaagagc
    16261 gcattgctca ggaagactgc cgtaatggtt tcctgttgga cggcttcccg cgtaccattc
    16321 cgcaggcaga cgcgatgaaa gaagcgggca tcaatgttga ttacgttctg gaattcgacg
    16381 taccggacga actgatcgtt gaccgtatcg tcggtcgccg cgttcacgcg ccgtctggtc
    16441 gtgtttatca cgttaaattc aatccgccga aagtagaagg taaagacgac gttaccggtg
    16501 aagaactgac tacccgtaaa gacgatcagg aagaaaccgt gcgtaaacgt ctggttgaat
    16561 accatcagat gactgcaccg ctgatcggct actactccaa agaagcggaa gcgggtaaca
    16621 ccaaatacgc gaaagttgac ggtaccaagc cggttgctga agttcgcgct gatctggaaa
    16681 aaatcctcgg ctaatcaagc cgcagcagat ctgctttcgg gcaggtctgc tcatccacct
    16741 caggcaatac ctctcacagc aattagttct tcttcctcac ttttccgcta caattatcaa
    16801 caagttgaat cgataagagg cggtaatgcg tcagactaaa accggtatcc tgctggcaaa
    16861 cctgggtacg cccgatgccc ccacacctga agcggtaaaa cgctatctga aacaattttt
    16921 aagcgacaga cgcgtggttg atacctcacg gttgttatgg tggccattgc tgcgcggcgt
    16981 gattttgccg ctgcgctcgc cgcgtgtggc gaagctgtat gcctctgtct ggatggaagg
    17041 tggctcgcca ctgatggttt acagccgcca gcaacagcag gcgctggcac aacgtttacc
    17101 ggagacgccc gtagcgctgg gaatgagcta cggctcgcca tcactggaaa gcgccgtaga
    17161 tgaactcctg gcagagcatg tagatcatat tgtagtgctg ccgctttatc cgcaatactc
    17221 ctgttcaacg gtcggtgcgg tatgggatga actggcacgc attctggcgc gcaaacgtag
    17281 cattccgggg atatcgttta tacgtgatta cgccgataac cacgattaca ttaatgcact
    17341 ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa ccggatctgc tgctgctctc
    17401 ttatcatggc attccccagc gttatgcaga tgaaggcgat gattacccgc aacgttgccg
    17461 cacaacgact cgcgaactgg cttccgcact ggggatggca ccggaaaaag tgatgatgac
    17521 ctttcagtcg cgctttggcc gggaaccctg gctgatgcct tataccgacg aaacgctgaa
    17581 aatgctcgga gaaaaaggcg taggtcatat tcaggtgatg tgcccgggct ttgctgcgga
    17641 ttgtctggag acgctggaag agattgccga gcaaaaccgt gaggtcttcc tcggtgccgg
    17701 cgggaaaaaa tatgaatata ttccggcgct taatgccacg ccggaacata tcgaaatgat
    17761 ggctaatctt gttgccgcgt atcgctaaag ctgagcggta aagaactgag cgccgtcgcg
    17821 aagagcctcg tcggcggttt tcatcatccg tgaataatgc aaaaaggcgt gcagcgtgcc
    17881 tgggtagagt ttgaactcac agggctgctg atgcgccgct aacgtctggt aaagcagacg
    17941 gctgtcatcc agcagcggat cgaactccgc cccggcaata aaacagggcg gaacttcgcg
    18001 agtgagatca ttattaaaca gacagtaata cggcgactcg cggtccgcgt cgttgcttaa
    18061 atacgcctct tcatacatct gcaaatcctg ttgcgttaag ccatcccaga caccgcccaa
    18121 cagacgacga gtcacggaat cccgtaatcc gtaaagccca taccacagca aaacacccgc
    18181 aactttaccg caatcgatct gtttatcacg caaccacaac gcactggcga gcgccagcat
    18241 ggcacctgcg gaatcaccgg caaagccaat gcgggacata ttgatttgat aatcctccgc
    18301 ctgctggtgg aaataacaac aagcagccac aatttcctct atcgcttgcg gaaaacgcgc
    18361 ttcaggtgaa agggtgtaat caataccaat caccgtacat tggctgtagc ttgccagcag
    18421 gcgcatgatg cgatcgtggg tatcgagatt gccgagaata aaaccgcctc catgcaaata
    18481 aaatagcgtc gctgggctat ctggctgcgg acaaaagaga cgtgtttcca cctgcccata
    18541 ttttgttgga accatgtaag ctctggttgc catttctgga gcgcccgcat tccagaatcg
    18601 gcgctcaagc gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg gccagggcgg
    18661 taaatccggc tgaagagtat tcacaacggt cttcatttca gcagaaataa ggtccagaac
    18721 aggtaatttg ttttccggct tcatacaaaa actcctttca aattacgtca ttgtaaggaa
    18781 ccactgccat gaaaatgcga tcccgcctgc tgatattgaa actggctgcg tctcgcgcgc
    18841 tcccgtcaga ttgtgttaac attcgccgct cagttaacca cccgtaaaaa caaccatgaa
    18901 atttcccggt aaacgtaaat ccaaacatta cttccccgta aatgcacgcg atccgctgct
    18961 tcagcaattc cagccagaaa acgaaaccag cgccgcctgg gtagtgggta tcgatcaaac
    19021 gctggtcgat attgaagcga aagtggatga tgaattcatt gagcgttatg gattaagcgc
    19081 cgggcattca ctggtgattg aggatgatgt agccgaagcg ctttatcagg aactaaaaca
    19141 gaaaaacctg attacccatc agtttgcggg tggcaccatt ggtaacacca tgcataacta
    19201 ctcggtgctc gcggacgacc gttcggtgct gctgggcgtc atgtgcagca atattgaaat
    19261 tggcagctat gcctatcgtt acctgtgtaa cacctccagc cgtaccgatc ttaactatct
    19321 acaaggcgtg gatggcccga ttggtcgttg ctttacgctg attggcgagt ccggggaacg
    19381 tacctttgct atcagccctg gacacatgaa ccagctgcgg gctgaaagta ttccggaaga
    19441 tgtgattgcc ggagcctcgg ctctggttct cacctcttat ctggtgcgtt gcaagccggg
    19501 tgaacccatg ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat ataacgtacc
    19561 ggtggtgctg acgctgggca ccaagtttgt cattgccgag aatccgcagt ggtggcagca
    19621 attcctcaaa gaccacgtct ctatccttgc gatgaacgaa gatgaagccg aagcgttgac
    19681 cggagaaagc gatccgttgt tagcatctga caaggcactg gactgggttg atctggtgct
    19741 gtgcaccgca gggccaatcg gcttgtatat ggcgggcttt accgaagacg aagcgaaacg
    19801 taaaacccag catccgttgc tgccgggcgc tatagccgaa ttcaaccagt atgagtttag
    19861 ccgcgccatg cgccacaagg attgtcagaa tccgctgcgt gtctattcgc acattgcgcc
    19921 gtacatgggc gggccggaaa aaatcatgaa caccaacgga gcaggggatg gcgcactggc
    19981 agcgttgctg catgacatta ccgccaacag ctaccaccgt agcaacgtac caaactccag
    20041 caaacataaa ttcacctggt taacttattc atcgttagcg caggtgtgta aatatgctaa
    20101 ccgtgtgagc tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg gcttgccgga
    20161 gcgtgaagac agcctggaag agtcttactg ggatcgttaa gttatcgtcg gttcgtaggc
    20221 cagataaggc gttcacgccg catctggcat tttgctctca atgcctgatg cgacgctggc
    20281 gcgtcttatc aggcctacat atccgcgcct attatccggc aaccactgtt taccccgtca
    20341 ccacttcacc cgccggtggc gtttccagca attccagcat agtacgggca atttcacgct
    20401 cgcccatcac tacctgattc gcaccacgtt cggtgatata cgccacttca tcgtcataat
    20461 gggcgcgggc gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcgc
    20521 ctgcttcata accgttggga atcgtcagga tcagccattt tgcacattcc agatgcgcca
    20581 gttgcataat ttcttcgttc gccgcattgc ccaatactgc gcggacaccg cgctctcgca
    20641 gctcatcaac acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca
    20701 atttctcccc cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa
    20761 tatccactgg gatctgcttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg
    20821 tttcggtctt cgccagatat ttctccagca gcgcaaacag gaccgggttg agcataatcg
    20881 acaaaatcgc acctgccagt accaggttct gcccggcttg cggcaacaga tccagcgcca
    20941 tgcccagtcc cgccaggata aacgcgaact caccaatttg cgccaggctg gcggcgatag
    21001 ttaatgccgt acgttgggag tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact
    21061 taccaaacag aataatcgcc agcgtcgcca gcactgccag cggttgctga atcagaatta
    21121 acggatcaaa caacatcccg acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg
    21181 gcagcgtatc gtgggcggca cggtgactca gttcagactc gttcagtacc atcccggcaa
    21241 agaacgcacc gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta
    21301 acgccagcgc cagcaccgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg
    21361 ccataatcca cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca
    21421 ccttgccgat ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt
    21481 ccatcattcc tgccactgcg ggcagcaaca ccagcgtcag aaccattacc aggtcttcca
    21541 caatcaacca accgatggcg atttgcccac gctgactgtc aattaattgc cgttcttcaa
    21601 gtgcgcgcag taacaccacg gtactggcgg tggaaagaca taaaccgaac acgataccgg
    21661 tcattaacga ccagcccagc acggcagaga gcgccatacc cagcagcgtc gccacggcta
    21721 tctgggcgat cgcaccggga atggcgatgg cctttaccgc catcaaatcc ttcagcgaaa
    21781 agtgcagacc cacgccaaac atcaacagaa tgacgcccag ttcagccagt tccggcgcaa
    21841 gcttggtatc ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat
    21901 atcccaccag aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca
    21961 caaggccgcc aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc
    22021 ctggtggtta ttgtccattt ttggccggga aaaccaaaat tacaggtaat agtttatgac
    22081 aatttcattg atgatgttca tgaataatta ttgaattttg cagaaaaatg gaattagctg
    22141 caaaaaaagc acggatcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac
    22201 tgtaaaacga tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt
    22261 tatcaggcag gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga
    22321 ctaactcgat gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca
    22381 tgccgaaagc aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagcagct
    22441 cctgagcgta aaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg
    22501 ttaaaacccc cgtccagtgc aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc
    22561 cgaggataga gccccaaatc acatatttcc gcccaatttt atcccctaca ggcccgccga
    22621 tcaccgtacc tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga
    22681 tagataatcc gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat
    22741 agaaatattt cgagaaaatg aggattaaca gaatgctgac cgccagaacc actttgttgc
    22801 gcggcagtgg attgataatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg
    22861 agtaccaacg gctgatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc
    22921 aggcaacgtt gcctttgcca taaggcgcga taatcaccgc cgccagcaag ggtcccaggg
    22981 aactgccaaa gttgccgccg acctgaaaga ttgattgcgc caggccatgc cgcccgccag
    23041 aagccatacg ggccacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca
    23101 gcgctgccgc cagcagaact gcgccaaaac tgccagccag cgcaagcagc accagaccac
    23161 ttaaggtaaa gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt
    23221 agccgaccac tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa
    23281 tctgcataaa tgtcagagaa aattctgact gaagcagagg ataaatcgcc agaatcagcg
    23341 attggatcat gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg
    23401 ttcgggcctt ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg
    23461 ccataggaaa gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt
    23521 acccgactaa catacctgta tgcgccgcct gaaggaagtc tcaacgctga atacagaatt
    23581 tctaatctgg atgcagattt atcttcaccg gacgcagact tgtctatgat gtcgcgtcat
    23641 actatttttc aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg
    23701 gcgtggcgtt agcactgtta accacattta cactggcgag tgaagctgct ctggcgtatg
    23761 agcaggataa aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt
    23821 ggcgcaatga atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc
    23881 gcaaagaggt tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca
    23941 ctggcgtgcc cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg
    24001 tgggctatga cgcgatggcg atcggtaatc atgaatttga taacccgctc accgtattac
    24061 gccagcagga aaagtgggca aagttcccgt tactttctgc caatatctac cagaaaagta
    24121 ccggcgagcg cctgtttaaa ccatgggcgc tgtttaagcg tcaggatctg aaaattgccg
    24181 ttattggtct gacgacggat gacacagcga agttaggcaa cccagaaaac ttcaccgata
    24241 ttgagttccg taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag
    24301 aaaagccaga cattattatc gcggcgacac atatggggca ttacgataac ggcgatcacg
    24361 gttctaacgc accgggcgat gtggagatgg cacgcgcgtt gcctgccgga tcgctggcga
    24421 tgattgtcgg cggtcactcg caagatccag tctgtatggc agcggaaaat aaaaagcagg
    24481 tagattacgt tccaggtacg ccatgcaaac cggatcaaca aaacggcatc tggattgtac
    24541 aggcgcatga gtggggcaaa tacgtgggac gggccgactt tgagtttcgc aacggcgaaa
    24601 tgaaaatggt taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag
    24661 acgggaaaag cgagcgcgtg ctgtacaccc cggaaatcgc tgaaaaccag caaatgatct
    24721 cgctgttatc accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa
    24781 ccaatggtcg tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc
    24841 ggttgattct ggcagcacaa atggatcgca ctggtgccga ctttgcggtg atgagcggag
    24901 gaggaattcg tgattctatc gaagctggcg atatcagcta taaaaacgtg ctgaaagtgc
    24961 agccattcgg caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc
    25021 tgacagccgt cgcacaaatg aagccagatt caggtgccta cccgcaattt gccaacgtta
    25081 gctttgtggc gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc
    25141 cagcgaaaac ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggctatc
    25201 cgcgccttga taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga
    25261 aagcgtatat ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg
    25321 tgagctggca gtaatccgaa agtgccggat gttcgcatcc ggcacaatgc ttaatcgcgg
    25381 cgggcgatat cagcaaattt ggcatcgagg atcttcgcca gatcgcctgc cgccagttcg
    25441 atatccagtc cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg
    25501 tcgataatcg ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat
    25561 cccgtcgaac gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct
    25621 tttgctactt ttttaaggtc cagttgacct gcgaccggcg taacggccac ggcaaggtgt
    25681 ttcatatcac cgttcactgc gaccagcagc gttttgtaga cctgatccgg attcaaacct
    25741 aattttttga cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg
    25801 atttgaaacg aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc
    25861 ctgccttaaa caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc
    25921 atcttgccta agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg
    25981 cttaaagtga cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat
    26041 attggccatt tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg
    26101 caccgaccgc gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga
    26161 gatttcgctg atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta
    26221 tttcattatt ttgcggcggc gcattcagcc accagctcat catttgttgc agcaaccgtg
    26281 cgttggtatg ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct
    26341 ggagcaacat ggcaatctgg ttttccgccc cttccagttc aatcacgggt ttatgttgtt
    26401 gcttcgccgc ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt
    26461 gtgcctgcgt cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg
    26521 agggagaaat gcccatctcc tggctaatgt gctgcagttt ttggagttgc tcctcgctaa
    26581 tgcgctcttc cagcgcctcg caggcaggca aattagcaaa aggcatatcg ttggtggaaa
    26641 catccgcctc gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggcgggca
    26701 ggggagccat atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc
    26761 cggggagggt gatatcgatg gcaggccagg agtaatgatt accgcgcagc gcggcccaaa
    26821 gcgtttttac ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg
    26881 cgcggggagg gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag
    26941 tatgttcttg tatcgctatt cacagggaag caacagttaa aaactatgaa acaggcaaca
    27001 agaaaaccga cgacacccgg atatattctt ctctatgaat atctggaacc gctcgatttg
    27061 aaaatcaatg agttagcaga gttgctgcat gttcatcgta atagcgtcag tgcactgatc
    27121 aataacaatc gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc
    27181 acagtcgatt tttggctaaa cctccaggca gcagttgatc tttgggaagt tgaaaacaac
    27241 atgcgcaccc aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt
    27301 gaagagcgtg caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat
    27361 aaggcgttca cgccgcatcc ggcatccgag taccattgcc tgatgcgacg cttacgcgtc
    27421 ttatcaggcc tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc
    27481 cggcagtcat gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta
    27541 cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg gtaatcgacg
    27601 agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca
    27661 aaataccggc ggcgaccgga ataccgatac tgttgtagat aaacgcgccg agcaggttct
    27721 gcttcatgtt gcgcagcgtt gcgcgggaaa tagcgagcgc atccgcaacg cccatcaggc
    27781 tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg ccgcccattg
    27841 caatgccgac atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg
    27901 ccacctgacg tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tctggcagca
    27961 ccccggctat cacctcatca atcccggctt ctttggcgat cgcattggcg gtggttgggt
    28021 tatccccggt caacatcacc agacgatatc ccgctttatg cagacgttgc agcgccgcca
    28081 cgctatcact acgcaacgga tcgcgtacag ccagcagggc taccgctttc ccgtcaaccg
    28141 ccagcagcac aggcgttgcc ccttgcgatg cctgagctga aatatctgct tcaatcgctt
    28201 tagtatcaac ttgctggtca tttaacagcg cctgattgcc cagcaataac gcatgacctt
    28261 cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggcagct
    28321 gcatatcgct tgctttatcg aggatcgccc gcgccagcgg atggctggaa ccttgttcca
    28381 gtgccgccgc cagacgcagc gcctgcgctt cgtcaaaatc agcaaatgtt ttcactgcga
    28441 caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg
    28501 tactggcgcg ttgtagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc
    28561 cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga caggcaataa
    28621 tcagcaccgt ggtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat
    28681 accagattgc cgcactgaaa agcgcaatca ccactactac cggcacaaat acggctgaga
    28741 ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc accatacgaa
    28801 taatccttga tagcgtggta tggctgccaa ccgcactggc gcgaaacaga acgctaccgt
    28861 cctgtaccac tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggataggtt
    28921 cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctactg
    28981 gcacgcggtc gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca
    29041 cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt
    29101 tctccagcgc cttagaagaa cgctgacgtg cgcgcgcttc cagcatatga ccgagattaa
    29161 tcagaccgat aatcatcgca ctggcttcat agtaaagatg ccgcgcttcc atcgggaacc
    29221 actgcggcca caggttgacg ctcatcgaat agagccacgc cacacccgta cccagcgcca
    29281 ccagcgtatc catcgtcgcc gcaccgttca gcaggctttt ccatgcactg cggtaaaaat
    29341 ggccgccggc gaaaaccatt accgccaggg ttatcaggcc gataaccaac cacaggctgc
    29401 ggttgtcagc ggtgaccatc atgttatcgc cgatcatccc ccagaccatt accgggatac
    29461 ccaccgccag tgcaacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt
    29521 cttgctggcg ctcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt
    29581 tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct
    29641 ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcaacgca ttttgtacgc
    29701 gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat
    29761 cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg
    29821 actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga
    29881 tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca
    29941 catccgcttg ttccacatcc gggcgctgtt cgagactttc tttcacgcgt ttaacgcagt
    30001 gaccgcagga caggccgtcc agggtcaggt cgatagtttg tgacataaaa cactccttta
    30061 agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa
    30121 ttaataaccc aggcgagtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag
    30181 gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag
    30241 gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa aaaggagtta
    30301 acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt
    30361 tcactcactt aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg
    30421 tcaactggcg gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag
    30481 tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc ttgcgttaga
    30541 agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt
    30601 taactcagtt atcgccttag agttgcatgg cggcaaaccg ctgtcgccac tggtaaatgc
    30661 tggcgctatt gccaccacca gcctgattaa cgctgaaaat gccgaacaac gctggcagcg
    30721 aattttacat atccaacagc aactggctgg tgagctggta gcgctctctg acgaagtcaa
    30781 ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc tgtactccgc
    30841 cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctccac
    30901 gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg ggggggtgaa
    30961 tccgctgacg catgaacgcg ttcttcaggc cgacaacgtg ccgtatattc tggccgaaat
    31021 gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg
    31081 caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc
    31141 gttctcacca ccgctggacg aagatggcaa cagtgttcgc ggtcaaaaaa tggtggcatc
    31201 ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt
    31261 aataacggta acaaacctct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg
    31321 gtgggggcgg ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc
    31381 tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc ctatgcgcgt
    31441 ctgggggcga gctatcccag caatggcggc attatcgact tctttcgtcg cggattaggc
    31501 aacggcgtct tttcgctggc gctctcgtta ctgtacctgt tgacgctggc ggtgagcatc
    31561 gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag
    31621 gaggagcacc ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac
    31681 tccttaagca accatgcggt agggcggctg gaggtgatcc tcgtcggcat taaaatgatg
    31741 atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc
    31801 tctgcgcccc ccagctccgg tgcgttcttc tcctgtatcg ggataacctt ccttgcctat
    31861 gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg
    31921 ccacgggcgt ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg
    31981 gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac cgccgtagcg
    32041 caggcagctt ctccgctgct cgggcatgtg ggttatgtga tcgtcgttat cggcgcttta
    32101 ctggcgacgg cttcagccat caacgcgaac ctgttcgccg tgtttaacat catggacaac
    32161 atgggcagcg aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc
    32221 tggggcaaca tcattgtcgt ggtgttgatt atgctgatga cggcggcact aaatttaggc
    32281 tcactcgcca gcgttgccag cgccaccttt ttgatttgct atctggcggt gtttgtggtg
    32341 gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt aggtacgttg
    32401 gtgatgttgt tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg
    32461 ttgatatgga ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgc
    32521 aataaaaccg tataacacct ctctgcgcgc agtactgcct gtactgcgcc tttgtcttta
    32581 attcaaatta cctttcttat tcctttaata aataagtcac aaaaaaatga tatatatgcg
    32641 ataaa
//
LOCUS       DAPGRN010000047        32592 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPGRN010000047 DAPGRN010000000
VERSION     DAPGRN010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.047"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(87..1484)
                     /gene="ccp"
                     /locus_tag="RHW33_002356"
     CDS             complement(87..1484)
                     /gene="ccp"
                     /locus_tag="RHW33_002356"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV1645852.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1889..3538
                     /locus_tag="RHW33_002357"
     CDS             1889..3538
                     /locus_tag="RHW33_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV1645853.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3589..4191)
                     /locus_tag="RHW33_002358"
     CDS             complement(3589..4191)
                     /locus_tag="RHW33_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1645854.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNTSS"
     gene            4711..5610
                     /locus_tag="RHW33_002359"
     CDS             4711..5610
                     /locus_tag="RHW33_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1645855.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPHLSEFLYQHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5659..6672
                     /gene="yhjD"
                     /locus_tag="RHW33_002360"
     CDS             5659..6672
                     /gene="yhjD"
                     /locus_tag="RHW33_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV1645856.1"
                     /translation="MTQENEIKRPTQDLEHEPIKPLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7084..8406
                     /locus_tag="RHW33_002361"
     CDS             7084..8406
                     /locus_tag="RHW33_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV1645857.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8588..10648)
                     /locus_tag="RHW33_002362"
     CDS             complement(8588..10648)
                     /locus_tag="RHW33_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV1645858.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10718..11485)
                     /gene="pdeH"
                     /locus_tag="RHW33_002363"
     CDS             complement(10718..11485)
                     /gene="pdeH"
                     /locus_tag="RHW33_002363"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV1645859.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLSPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11717..12646
                     /gene="kdgK"
                     /locus_tag="RHW33_002364"
     CDS             11717..12646
                     /gene="kdgK"
                     /locus_tag="RHW33_002364"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV1645860.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGERLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12742..14238)
                     /locus_tag="RHW33_002365"
     CDS             complement(12742..14238)
                     /locus_tag="RHW33_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV1645861.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(14459..15745)
                     /gene="dctA"
                     /locus_tag="RHW33_002366"
     CDS             complement(14459..15745)
                     /gene="dctA"
                     /locus_tag="RHW33_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV1645862.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15928..17916)
                     /gene="hmsP"
                     /locus_tag="RHW33_002367"
     CDS             complement(15928..17916)
                     /gene="hmsP"
                     /locus_tag="RHW33_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV1645863.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(17998..21471)
                     /gene="bcsC"
                     /locus_tag="RHW33_002368"
     CDS             complement(17998..21471)
                     /gene="bcsC"
                     /locus_tag="RHW33_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV1645864.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVQGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWSSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTRESTNADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKTQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21453..22559)
                     /gene="bcsZ"
                     /locus_tag="RHW33_002369"
     CDS             complement(21453..22559)
                     /gene="bcsZ"
                     /locus_tag="RHW33_002369"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV1645865.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQATCTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22566..24905)
                     /gene="bcsB"
                     /locus_tag="RHW33_002370"
     CDS             complement(22566..24905)
                     /gene="bcsB"
                     /locus_tag="RHW33_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV1645866.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAIAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDVVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24916..27534)
                     /gene="bcsA"
                     /locus_tag="RHW33_002371"
     CDS             complement(24916..27534)
                     /gene="bcsA"
                     /locus_tag="RHW33_002371"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV1645867.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27531..28283)
                     /gene="bcsQ"
                     /locus_tag="RHW33_002372"
     CDS             complement(27531..28283)
                     /gene="bcsQ"
                     /locus_tag="RHW33_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV1645868.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28295..28483)
                     /locus_tag="RHW33_002373"
     CDS             complement(28295..28483)
                     /locus_tag="RHW33_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV1645869.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28756..30327
                     /gene="bcsE"
                     /locus_tag="RHW33_002374"
     CDS             28756..30327
                     /gene="bcsE"
                     /locus_tag="RHW33_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV1645870.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30324..30515
                     /gene="bcsF"
                     /locus_tag="RHW33_002375"
     CDS             30324..30515
                     /gene="bcsF"
                     /locus_tag="RHW33_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV1645871.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30512..32191
                     /gene="bcsG"
                     /locus_tag="RHW33_002376"
     CDS             30512..32191
                     /gene="bcsG"
                     /locus_tag="RHW33_002376"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV1645872.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNILTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32278..32385)
                     /locus_tag="RHW33_002377"
     CDS             complement(32278..32385)
                     /locus_tag="RHW33_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV1645873.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         7690 a         8935 c         8190 g         7777 t
ORIGIN      
        1 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
       61 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
      121 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
      181 cctacctgat agcgcaacat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
      241 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg acgcaattta
      301 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
      361 ttaaagtctt ttttcagccc caacggttcg aaggaacgtc cgccgagaat aattccacca
      421 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
      481 agcgcattct catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
      541 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggatagact
      601 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
      661 gattttgacg ccatttcaat cgggttcaac ggcggtccac cagcctgatc ctgcaatgtt
      721 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
      781 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
      841 gcgttcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
      901 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
      961 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     1021 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1081 cagtgtagcg cggtataacg cgttggcggc atagtttcat actgcatcac ccattcaatc
     1141 ttattcagat cgctttgcga aacgggttta tcagccagca gagccgcacg cacggcctcg
     1201 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     1261 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca gcctttttcg
     1321 cggagaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     1381 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     1441 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     1501 tggtgagtta ttgtgaccca gaaaattttc cagaatgctg caaacgtctc tgcgagacaa
     1561 agccatcata aaaaacaggg agatacactt tttgatagga atcaatcaat tacatgaata
     1621 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     1681 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acacgaaatc tgtattttta
     1741 taaattccat ggatattagt gcgttatatt ttttgacagg ttaataaacg tgatccaccg
     1801 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag cccgataatt
     1861 agcgacgaat ttcggaggtt ggatccttat gctcaatcag aaaattcaaa accctaatcc
     1921 agacgaactg atgatcgaag tcgatctctg ctatgaactg gacccgtatg aattaaaact
     1981 ggatgagatg atcgaggcag aaccggaacc cgagatgatt gaagggctgc ccgcctctga
     2041 tgcgctgacg cctgcggatc gctatctcga actgttcgag catgttcagt cggcgaaaat
     2101 tttccccgac agtaaaacct ttcccgactg cgcacccaaa atggacccgc tggatatttt
     2161 aatccgctac cgtaaagtgc gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa
     2221 tcacttctgg ctgccggagg tctactccag cgagtatgta tcggacccgc aaaattccct
     2281 gaaagagcat atcgaccagt tgtggccggt gctaacccgc gaaccacagg atcacattcc
     2341 gtggtcttct ctgctggcgc tcccgcagtc atatattgtc ccgggcggcc gttttagcga
     2401 aacctactat tgggattcct atttcaccat gctggggctg gcggaaagtg gtcgggaaga
     2461 tttgctgaaa tgcatggccg ataacttcgc ctggatgatc gaaaactacg gtcacatccc
     2521 caacggcaac cgcacctatt atttgagccg ctcgcaacca ccggtttttg cgctgatggt
     2581 ggagttgttt gaagaagatg gtgtacgcgg tgcgcgccgc tatctcgacc acctgaaaat
     2641 ggaatatgcc ttctggatgg acggtgcaga atcgttgatc cctaatcagg cctatcgcca
     2701 tgttgtgcgg atgccggacg gatcgctgct caaccgttac tgggacgatc gcgacacgcc
     2761 gcgtgacgaa tcctggcttg aggacgttga aaccgcgaaa cattctggtc gcccgcccaa
     2821 cgaggtgtac cgcgatttac gcgcgggggc ggcctccggt tgggattact cttcccgttg
     2881 gctgcgtgat actggtcgtc tggcgagcat tcgtaccacc cagttcatcc ccatcgatct
     2941 gaatgccttc ctgtttaaac tggagagcgc catcgccaac atctcggcgc tgaaaggtga
     3001 gaaagagaca gaagcactgt tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg
     3061 ttacctctgg gatgatgaaa acggcatcta ccgcgattac gactggcgac gcgaacaact
     3121 ggcgctgttt tccgctgccg ccattgtgcc actctatgtc ggtatggcga accatgaaca
     3181 ggccgatcgt ctggcaaacg ccgtgcgcag tcggttactg acacctggcg ggattctggc
     3241 aagcgagtac gaaaccggtg aacagtggga taaacccaac ggctgggcac cgttacaatg
     3301 gatggcgatt cagggattta aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg
     3361 aagctggctg aagacggtga atcagttcta tctggaacag cacaaactga tcgagaaata
     3421 ccatattgcc gatggtgttc cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg
     3481 gtttggctgg actaacggtg tggtacgccg tttaattggt ttgtacggcg aaccataata
     3541 tttttacagc cagccgctaa cttcctgctg gctgtaaaat tatcctcttc aggaggaggt
     3601 atttaacatc attgccgcct gggtgcgatt tttcacttcc agacgacgat acagggattc
     3661 cagatgcgct tttaccgttc cggtactgat attcagcgcc ctgccgatct ccttatttga
     3721 ttcgcccgcc gctaacatgg ttaaaatctc ccgctggcgg gcgcttaacg atttgagatc
     3781 tttaatgtcc ttttccggcg tcgtccgcca gtctccaggc agaaacatca tccccatcgc
     3841 tgcactattt accgccaacg caaatgtctc gacggttgaa tcacgaggca caatggccag
     3901 cacattaaaa tggataactt cctgtaacca ccgtttattg caatccgtcg ccgtaattaa
     3961 caccttaacc tcaggaaatt gcaccacggt tttttgcagc aaccagtagc aaaactcacc
     4021 atcctgatcg ccatcgagca taactaaggc ttcagggtaa ctttccagct tttgccataa
     4081 ctcgtctgcc tgactggccc cctgaatact cactcctgga atacgctgct gtaaactgat
     4141 tttcattcca tgaataaata ttgactgcct gtcaaacatg actatttgca tgactgaatc
     4201 tccacctgaa tacgttaaaa agacttaagt agtggaaggg tattacccgc gagaaaaaat
     4261 aagaattcgc catttggcgg tggccattct acagagatga cgcgtagaaa atagttaccg
     4321 atataaatag ttacagctaa acgcctgaaa ttacatgtcg agggcactat ttaaaacaat
     4381 tttgaggatt tccttatatt ggtggttagt acgcatgcaa ttaaaaatga aattccgcga
     4441 ccacaagcca aaataacaaa tggcaatgag acaaaaataa gcacaaatag ccaatacgtc
     4501 ctctattcac tttaaaggga atcgctgaaa aatacgctct gtttaagggg attcaccttt
     4561 ctcagaaagc tattccgccc ttttcctgct gagaaatcgc cacattcgtc atgacaacat
     4621 tgtgaaaccc ggcattagat gttagaaaaa acaataaaca atgcgatatg cgcgttactg
     4681 tttcgctgtg aacaacaatc ggtcaaagaa atggataaaa ttcacgcaat gcagttgttc
     4741 atcaaagtcg cggagctgga aagtttttcc cgcgcggcgg atttctttgc tttgccaaaa
     4801 ggaagtgttt cgcgccagat acaggcactg gaacatcaac ttggcaccca gcttctccag
     4861 cgcaccacgc gacgggtcaa actcacgcca gaaggcatga cctattatca acgagcaaaa
     4921 gatgtgttga gtaatctcaa cgaactggac ggtctgtttc agcaggatgc caccagtatc
     4981 agcggtaaat tacgcatcga catcccgcca ggaatcgcga aaagcctgtt actgccgcac
     5041 ctgtcggaat ttctctatca gcatccggga attgagctgg aactgagtag ccatgaccgt
     5101 ccggtagata ttcttcatga tggttttgat tgcgtgatac gcactggcgc gttaccggaa
     5161 gatggcgtta tcgcccgtcc cctcggcaaa ctgaccatgg tcaactgtgc cagtccgcac
     5221 tatctgacgc gctttggtta tccgcaaagc cccgatgatc tgacttcaca cgcaatagtg
     5281 cgttatacac cgcacctggg tgtacatccg ttaggttttg aggttgccag cgttaatggc
     5341 gtccagtggt ttaagtctgg cggcatgttg acggtaaaca gtagcgaaaa ctatctcgcc
     5401 gccggtcttg ccggtctggg gattattcag atcccgcgca ttgccgtgcg cgaagccctg
     5461 cgtgccgggc ggcttattga agtgttgcca ggctaccgtg ccgagccgct ctccctttcg
     5521 ctggtttatc cgcagcgtcg ggagctttcc cggcgtgtaa acctgtttat gcagtggctg
     5581 gctggcgtaa tgaaagagta cctggactga ccgactatac tttttaagaa tgaccacgac
     5641 agaaggacaa aagagcggat gacgcaggaa aacgagatca aacgtcccac ccaggatctg
     5701 gagcacgagc cgattaagcc gttggataat agcgagaaag gcggcaaagt tagccaggcg
     5761 ctggaaaccg tcaccaccac tgccgaaaaa gtccaacgcc aaccggttat tgcgcacctg
     5821 attcgcgcga cagaacgctt taacgatcgg ctgggtaacc agtttggtgc ggctatcacc
     5881 tatttctcat ttttgtcgat gatcccgatt ttgatggtgt cgtttgccgc agggggcttt
     5941 gtgctggcct cccatccgat gttgctacag gatatcttcg acaaaattct gcaaaacatc
     6001 agcgatccga cgctagccgc tacgttgaaa aacaccatca acaccgccgt tcagcagcgt
     6061 acgactgtag ggcttgtcgg cctggcggtg gcgctttatt ccggcatcaa ctggatgggt
     6121 aacctgcgtg aagcgattcg cgcccagtcg cgcgatgtct gggaacgctc gccgcaagat
     6181 caggagaagt tctgggtaaa atatctgcgt gattttattt cgctgattgg tttgttgatt
     6241 gcgctgattg tgacgctttc gatcacctcg gttgccggtt cggcgcagca aatgattatt
     6301 agcgccctgc acctgaacag cattgagtgg ctgaaaccga cgtggcggtt gattggcctg
     6361 gcgatttcca tcttcgccaa ctatctgctt ttcttctgga tcttctggcg gttgccgcgc
     6421 caccgtccgc gcaaaaaagc cctgattcgc ggaacatttc tggctgctat tggttttgaa
     6481 gtgattaaaa tcgtgatgac ctacaccctg ccatcgttga tgaaatcccc ctctggtgca
     6541 gcatttggtt ccgtgctggg gctgatggca tttttctact tcttcgcccg tttgacgctg
     6601 ttttgcgcgg cgtggattgc caccgccgaa tataaagacg acccgcgaat gccggggaaa
     6661 acacagcctt aaaacaggcc gggtgcagtt caaaacgcct tatccggcat acgaatttat
     6721 tcggcctagc tccccgtagg ccgaataaga tgcgccagca tcgcatccgg ctataatgcg
     6781 cacataacct cttgaaactc tttcccagag cctcttcagc catctattca ggagcaaaca
     6841 atttcatgcc aactcataac cccagcatat aaatccagtt ggtaactttt atttaacctg
     6901 aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa gaaaaatcac
     6961 tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat ttgttacaca
     7021 ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa ataagaaaag
     7081 attatgcaag caacagccac aacactcgac cacgagcaag aatacacgcc gatcaactcg
     7141 cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt cgacttttac
     7201 atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca gggcgatcca
     7261 acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc gcgccccatt
     7321 ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac gctggtcgcc
     7381 tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg ctatgccacg
     7441 attggtattt tcgccccgct gctgctggcg ctggctcgat ttggtcaggg tctgggctta
     7501 ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc gcgcaaacgt
     7561 gcactgtatg gctccttccc acagctgggc gcaccgattg gcttcttctt tgccaacggc
     7621 actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg gggctggcgt
     7681 gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg cgtgtcgctg
     7741 catgagtcac cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa gatcccgctg
     7801 ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat tatgctggca
     7861 acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac cgccgccgcg
     7921 ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat ggcagttatt
     7981 ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg tcgccgtaaa
     8041 agcatggtaa ttatcaccac gctgatcatc ctgttcgcgc tgttcgcctt taacccgctg
     8101 ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt aagtctgatg
     8161 gggctgacct tcgggccgat gggcgcgctg ttaccagagc tgttcccaac agaagtgcgt
     8221 tacaccggag catcattctc ttacaacgta gcgtcgattc tcggggcttc cgttgcgccg
     8281 tatatcgcag cctggttgca gactaactac gggttaggtg cggtggggtt atatctggcg
     8341 gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg acatcagtcg
     8401 ttgtaataag agtgaaaagc ctgatgcgct acgcttatca ggcctgaatt tccatacaat
     8461 atattgaatt cgcaaggatt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
     8521 caaagcacac tttgccaaca atctaaatct gtttaattgc cggatgtcag acatccggca
     8581 attatcttca cttcttcatc tgcgacaaaa tcgtccggca ctgattagcc tccccttcgg
     8641 aaggtgagat cagtgccagc aacgctgccg ctggcgtaac cagcgttgcc agtgccgcag
     8701 caacagcacc acgggcaatc aacggtccgg ctttcactcc agcctgcgga tttttaaacg
     8761 tcccacgcac atacagcggc gaacgcagtg tgataatccg aattccttta ctctccggat
     8821 caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaacg ttaatcaacg
     8881 cgttctcagt atcgaaagca aaaatctgcg ggcgtgccac gccgttggca atattcagat
     8941 tcgccgccgc gcagttcacc cgcacctcat catcaccaaa tatcgcaccg acaatgtagt
     9001 tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg tcattcatca
     9061 acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta ccgctaccgc
     9121 gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc acatcgggca
     9181 tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc atcggctttt
     9241 tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca aatttcagcg
     9301 gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg ctaatcggca
     9361 ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc atgacatccc
     9421 atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga acgctttttt
     9481 cgcccttctt ctgttcagac cgtttcgact tttcagcccc tttcccggaa tcaacgccaa
     9541 tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct tccagttttg
     9601 gtcgtggctt gccggtggtg tagatcagag aaccgtggat atcgctatcg ccaatgcgcc
     9661 cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc gctaccagcc
     9721 gaccatctgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt tcatagagat
     9781 cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc ttcattgggt
     9841 cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca gcctgtaccg
     9901 gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc gtaccagtga
     9961 gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aacgtaatcg cccacctttt
    10021 ctttatccgc tttacctttc gatccagtaa cttcgctgaa cggcagtggc ttgcctaacg
    10081 gatcgacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg atccgccctt
    10141 gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca ttcgcgtctt
    10201 tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag aggcgaatca
    10261 ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg gtcagcagcg
    10321 ccagcggggc cagcgttgcc tctacgcgtg gcaaatgcac catcgtgact tcgggaatat
    10381 ccggtggatt gccaagaatg atgtcttccg cgtgtacatg gggccacggc acccagctgc
    10441 gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagatcgcca cggatagcga
    10501 acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt cgattccagt
    10561 caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc cctgaaatcg
    10621 cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc ctgtgccagt
    10681 cctaaagata gtccagccag gcggaaaatg aggcagctta tagcgccaga accgccgtat
    10741 tcagcgtttc tatcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg aatgcgggcg
    10801 agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc accccgcgac
    10861 aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc ggcgactgac
    10921 gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact tcacttagtg
    10981 cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca aattcacaca
    11041 tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg aaacgcagcc
    11101 agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg atgagcgtag
    11161 ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg gctttttgcg
    11221 ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg atttcagtaa
    11281 aatagcgatc cggcgacagg cgttgcgaag ggttcaaggg atgcgtgacc accgttaata
    11341 gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg taagcacgct
    11401 cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca gggttgctta
    11461 ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg atgctgcccg
    11521 gactcgacaa agattatcgg cgcgcaaggg cagaacttta ccgcctggcc ggggtaaatt
    11581 tcccctctcc acaacggaat ctttcacgaa tacgttctgg ctcaaaaaaa taatggaaca
    11641 ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta attttttaca
    11701 gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca tgattgagct
    11761 ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gatacactga atacttccgt
    11821 ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa cggcgctggg
    11881 aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg ttgatacttc
    11941 cctgacccaa cggatggaaa accgtctgcc gggcctttac tacattgaaa ccgacagcac
    12001 cggcgagcgt acgttctact actggcggaa cgaagccgcc gccaaattct ggctggagag
    12061 tgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct acctgagcgg
    12121 gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc tgctgcgcga
    12181 atgccgcgcc aacggcggaa aagtgatttt cgacaataac tatcgtccgc gcctgtgggc
    12241 cagcaaagaa gagacacagc aggtgtacca acaaatgctg gaatgcacgg atatcgcctt
    12301 cctgacgctg gacgatgaag acgcgctgtg gggtcaacag ccggtggaag acgtcattgc
    12361 gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg attcttgcct
    12421 ggtgtccatt gctggcgaaa ggttagtgga tgttccggcg gtgaaactgc cgaaagaaaa
    12481 agtgatcgat accaccgcag ctggcgactc tttcagtgcc ggttatctgg cggtacgttt
    12541 gacgggcggc agtgcggaaa acgcagcgaa acgtgggcac ctgaccgcaa gtaccgttat
    12601 tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc agcatatgca
    12661 aaaggctgga taaagagtta cccctctatc cagcccatcc ataaatacct gatggttgtg
    12721 ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt caggatgtac
    12781 gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga tttgatccca
    12841 ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca gcagtattaa
    12901 cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca cagtcaggct
    12961 gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga caacctgatt
    13021 ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg cataggcggc
    13081 aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact cttcttccgg
    13141 cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt tgctgttcag
    13201 cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca gcacacggca
    13261 gtcaaatcca agaccgatgt ctttgctgtt actggcgctt aacgcttgct gaacatgcca
    13321 gaacagcgcc tcacgggcca ggtccgcacg ccagtagcgc agcagtgcgg ctgattcacg
    13381 aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca ccgcgtcagt
    13441 cataatactc acagcttccg cacgcagcgg agaaagcgtc ggcaccggag ccggtgtttc
    13501 acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg agcgcgcatc
    13561 cacgtttccc accaccagca gcgtcattgc atccggggtg taccatttct gatagaaatc
    13621 tttaatcttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt gacctaacaa
    13681 ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag gccaggttgc
    13741 caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatag tcagtttgcc
    13801 agtggcattt gccagataag agagcgcctc tttcagcaaa tcgttacggt tattgggcaa
    13861 actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg ggcgtttagg
    13921 gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac cgctttgcgt
    13981 tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt cggcgagcga
    14041 accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg gggtggtcag
    14101 cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat caggctggag
    14161 cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg ctaaaagtcg
    14221 aatttttgtg ccctgcatgt gaacccctga tcaactatcc tggtaataaa aaaactgccc
    14281 gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa gaacatgaaa
    14341 atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc aggcacaggg
    14401 gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag tgatgagatt
    14461 aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca tcgtccagtt
    14521 ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg ccgttaccga
    14581 ccaggttagt cagcgcacga gcttctgaca taaagcggtc gataccgagg atcagcgcca
    14641 gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg ataaagccac
    14701 tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac agcgtgattt
    14761 ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc gccattgtca
    14821 ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc agccccacca
    14881 ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc gccgactcgg
    14941 aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata aatttgaaga
    15001 tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac aggatacagg
    15061 taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg ccgtatttac
    15121 cgatggtaaa cgccattgcc ccgaacgcac caataggtgc cagacgcatg atcatattga
    15181 tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt tggcctttgc
    15241 tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc tgcagaatgt
    15301 taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg aaggcgacaa
    15361 tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca agcgttgccg
    15421 gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga ccaataatca
    15481 gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta cgaccgaccg
    15541 ccttcatgct ttccatgccc gcgatgcccg ttacgacggt acaaaagatg acaggagcga
    15601 tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc tcgcctattt
    15661 caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc tgaaagtaaa
    15721 ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag gtcttgtaag
    15781 gttatggggt acctgcggcc ttaaaataac acccagacaa catcacagaa atgtacctgg
    15841 atcataattg aaacaaaaag gttaaaaagt ttgagctggc tcgcacaaac cagcactttt
    15901 taaagttttg taatcagttt ggggtagcta cttttcttcc aggtaactct cttcgaagat
    15961 ttcaataggg agtgggcgag caaaaaggaa gccctgggca ataccaacgc ccgcttttgc
    16021 cagccagtcg cgttgtgctt cagtctccac gccttcggca atcatttgta agttcaggct
    16081 ctgcgccagc atgataattg cagcaatcat gctgctatct tccggcaagc cttcaacaaa
    16141 cattttgtcg attttcagaa cgtcgattgg caacgatttc atatgctgca gctgacgcag
    16201 ccctgcgtag cccatgccga aatcatccag cgccacccga actccggcat tacgcagcgg
    16261 acggaggatt gccaccgcag catgagggtc gtcaatacgt cggctttctg tcacttccag
    16321 aatcagtgtt cccggctgaa tgcgatagcg ggttaacagt tccagcatat ccgccaccat
    16381 atttgggtgc atcagttgca gcgctgagag gtttaccgac aagggcagca taatgccgcg
    16441 ctcttgccag gcagccaaca gtcgacagga ctcttccagc acccagtgac cgacggtaac
    16501 catcagccca cagcactcaa tgcgatcgat taagccatcc ggcaggtccc aactgccatc
    16561 cggttgctgg atacgcagta acacttccgc actgaccagt ttaccgctgg tcatctcgac
    16621 ctgtggctgt aaccaaatag caaactgatg attttccaat gcattaagga tatcgctctc
    16681 ttccgtcaac cgcttctggg cagcttccat ctgctgcgga tcaaagaact gaatctgatt
    16741 cttgccttta tggcgagcgg taaatgccgc agaaatagcg cgactgtaaa gctgttcggc
    16801 ggtgagatcg ccgtagaaca tcgccacgcc aatgctacag tgcggacgga gttgaatacg
    16861 ttcaatcggc aggcgctcgc tcatgatagt gagcacttgc tgacctaaag tgattgcgtg
    16921 ccacggttcc tgtacaccgt tggcaatgac agcaaagtca taaccgctaa tctgcgcgag
    16981 gatcatacgt ggcgacagta ccgatttgag tttttccacc agcgtcagca gcagaatttc
    17041 tcgttgcgcc tctttcagca cgcccgcagt atcacgcaga gtttcacagg tgatgatcat
    17101 cagcgcggtg gtttgtttac gcgcgacaac ctgctccagc atctccatca gcaaggcttt
    17161 gttcggcaaa tccgacaccg ggaagcgcat cgcattttcg ttctgttctt cataatggcg
    17221 ctgcagcaat tgctggttga ggttgtaact gcgcaccaac ataccgattt catcatcctg
    17281 atgcagacgc ggtaatgcca gttggtgacc aacaagctcc tgggctggga tggcgttaag
    17341 ttcgcgagca atattgcgta acggatgcaa aatcaggcgg ttaatgcacc agctaatcgc
    17401 caccgtcaat attaacgaca aaagtaagta aatggtcact aacgttgaga gggtgctcat
    17461 cacgaactta tacatacgga aggaatccgc ctgtagcacc agataggcaa ttggctgcgg
    17521 atttgccgga cgttcgagcg agtagacgcc cagcgagatt tgaaccggta gctcaaacag
    17581 gcgagtaacc attaccggca ccggacgctc tggaataaaa cttttacgca gcgcctggaa
    17641 ctggttaggc agcactacat cggcacggct gaccacgcct gccggtttaa tgctggcaag
    17701 aatagcttcc gcttcgggaa tatcaccttt caaaatggct gaagataaag gttcgcggac
    17761 agagcgagca atgctttcca gttgcgtagc cgtgttatag cgattctgct ggaccagatg
    17821 gaacagcaaa acggtgcaaa aaataaaaac gaacaccagg acaacggctg ccaccattgc
    17881 catctgcttg attgttaacg agcgacttac gcgcaaattg tctccccccg gaaatcccag
    17941 attgccgccc gagtataccc gatcgcagcg gcattaagag aggcgctatc tgaaaactta
    18001 ccagtcggcg taaggtatca gcggctgcgg cggtaaatcc atgtcaccct gccatccggc
    18061 ggcggaataa cgtacgtaga gcaggaaatg gctgggggcg taatctttcg cctgctgaat
    18121 atcaattgcc gtgccgacaa accagttgga agtaacacgt cgttcaagta atgcccgcgc
    18181 cgtatagccg aagccctgac tgctgccgcc atcgttggat tgccgcgcag cttcttcctg
    18241 ccagtcggtc gggatcagat tcatcagcgg ataacgcggc atggttttgg tgcgtgaatg
    18301 cgaccacgag ccagacgccc ccagctccca cgaccagttt tccgtgcgct cccgccacat
    18361 caccggtatg gcaaacgaca ggtattcctg cgggctgtag taaccgccct gaccgagtga
    18421 gtagccactc agatctttgt cgtaatgcca gatcatgttg ttcaggccga ttgtgacgcg
    18481 gcgattgttc tggttaatga ccttatagta atagcccgtc atccagcgca cgcgccagtt
    18541 atcttcgaca tttttaccgg ttaactggtc gccactaagc gatgcccaga cgccgtttgc
    18601 ttcaccttta tcgtagctca ggcttagccc cacgccgtcg gcacgcacgc cgccccattt
    18661 tttcccggta ttgctcgggg agtctttttg cccaccaaag gccagcaaag aactggagat
    18721 gggccgacgg tgggcgttaa cggtgtaacc cagcggcccg atatcatcgc tgtaactgat
    18781 gccgccgacc acatccacca cgttgaagcc catcggcgtg gtgccgatat cccagctcca
    18841 gacgtcattt cgccagccga ccgccacgct ggcaccggaa tccgactggc tgcggttgcc
    18901 gctacagtcc tgtaatgtac aggtgcccca gttgtcatcc catttgccat cggcattagt
    18961 ggagaaactg ccgacgttca tattgacgaa atcactgcga aagaacatcc gcccgtcaga
    19021 atacggcgca tccacctgca acatggtagt gtgcgctttc agatcggagt aaccaccggt
    19081 accgctcgaa ccccagtaat catgctcgag ggtgacgtta agatcctgct ggcgatagag
    19141 gtccgccgca tcgctgcgca cgccgcgttt cagccagtca tctttctcgt cgttacgggt
    19201 cagtcgggta aaggtgtcgt tatcctgcgg acgcgtcgta gtcacaccgg atgcgaccat
    19261 ggcgtctttg taggtttcca gcgcctgctt tggatcgccc gcctgcgctt caaacttcgc
    19321 accgtcgcgc agtaccatcg cgctttccat cgacggcggc tgagtttttg cctgcgggat
    19381 cagcttgtta aacgtctgct gcgccgctgc ggtatcgcca agctgcgcct gcgccagcgc
    19441 cacgcgccgc tgtgtgttca gcgaggcgtt atcggtggca ggcagtttcg ccagctggct
    19501 acgtgccgcc gctttgtcac cagcagcaat atccacttcc gtcagaccaa gaatggcatc
    19561 ggcgttagtt gactcccgcg tcaggacatt ctgatatgca gcgcgggcgg cggtgtaatc
    19621 acgtcgctgc tgcgcccagt cagccagcgt gaggtcaata cgcgaggaag gcggttgctg
    19681 gcgcagcatc gcttccgctt cagcttcttt gccgctttct cgcaggcgat tagcggtttc
    19741 cagcacctga tcgctttgca gtcgattaac cagctcctga atattgctgc tccactgcgc
    19801 acgcggcagg ctgttgatat gcgccagcgc cgctctgtcc tggtcatgac cagagaggta
    19861 cagcccgtaa gcgtaaacct gctccgggtc gttcggcttc tgctgcgcca gattgcgcat
    19921 taacgtatcg gcctggctgc gttgtccggc ctgccagaga tcctgcgaaa gtcggtaagt
    19981 aatccatacg ctgccggggt ccagcgccag tcgttgccgc tgaagtgctg ccgcctgcgc
    20041 ccatttgccc tggttttcca gtgcctctgc ctgctgtgcc agacggtcgt tttgcaggct
    20101 gcgttcgata tcatcaatgc tacgccgctg actggcagag agcgaggcga taaacgcttc
    20161 agctttttct ggcgattgct ggcggtaaat atttgccagc ccgcgcacgg cgttagtgtt
    20221 gccgctgtcc atacgcaagg tctgctgata ataacgttcg gcggcgggat aatcttttcg
    20281 cgccatcgcc acatcgccca gcccctgcac tgcataactg tcggtgttat cgacattacg
    20341 cgcctgctgg aacaggcgtt ctgcccggtc aggattattg gctttcagcg cagcatcgcc
    20401 ctgctggatc gccagccagt agcggtttac tttcagcaga ctgttccatt tgtcgttgtt
    20461 gctgctgtgc ggatccagtg cgagggcttt ttccagattc gccactgcat tggcgcgatc
    20521 gcctttctgt gaatatgcct ggcccagcgc ccccagagct tcactgtctt tcgggtttgc
    20581 ccgcactgcc tgttgtagtt cgggaatggc tttacccgcc ataccagagt ccaccgccgc
    20641 taaaccttgc gcacgagcgc ggaaagcagg atcggccagc tgtttttgct gttctgccag
    20701 ttgcgattgc gcagccgcca cgctatcgcc atcactaaag atcgaaagat attttttcag
    20761 cgccgacaca ctggcatcac tgacgggcat gtctttaatc tgcccgtacc agattttaga
    20821 ggccccttcg cgcccggcgt tcgatttcgc catctgttcc aggacggcaa aaccttcgtc
    20881 acggcgatcg ctactaaaca gcaatagcgc cagattgttt tgcaggcccg tattgcccgg
    20941 tgcatccgca ttgatgcgtt ttaactgatt aatcgcttcg ccacggcgag ccggaatttt
    21001 cgccaccgta ctccagtact cgacagcaat gtcaccttcc ggcggcgcac cgttgaacag
    21061 tttgttgtaa ctcgccacag cttcttccgc atgaccggta gtcgcctgca atcttgcctg
    21121 ttgcagtgcc tgacgaccat ccggcgtgga aagtagcatc gtagtccgcg acgatttata
    21181 cgcatttgaa ctcggcgcta actgcgacag ccgatcgagt tgtttttgcg cgccatcaat
    21241 atcgccctga cgtaacaaag aacggaaacg ggcggcaacg acgtccgggt tattcggatc
    21301 aataagttcc agccgataca acgactgctg caccaggtct tcacgatggg tcgcttcgcc
    21361 taaccgaact tgctccagca actgttgctg agcggttggt gctgcctcga ccatcggcat
    21421 gacggccaga ccgagggaaa gcgtgaatat gtttagtgtg aatttgcgca ttcctggccc
    21481 cagtcaggta ataactcacc ttttgtcgag aagcggaaac ggtgttgatc ccagccttgt
    21541 ccaaacaggg tcagcacata gttgtaatag gcatcgctgc cgggaaagtt atcggccacg
    21601 cgctggcgct gaacggcctg cgcatcacgg ttttgtaaaa agggcagcat ggcggcagaa
    21661 aaaccgaccg gtcctttacc ctgcgctttc cccgtagcca catccacttt ttccggcgga
    21721 taaccgtttt tctcagtgaa tgtcgccatc ggtttaaacc ggttgagcat ccgcgctttc
    21781 tgcggatcgc tgtcaggcat catgcctacc cacatgtaaa cgcggatagc gtcgtagctg
    21841 ctgatcaatg ttttttcggc ttttagctgc cagcctttgt ctttctcata gcgcacccag
    21901 tctggcgaaa agcctttcgg ggcggtttcc agcaataaac gttgattggt ttcgcgcagc
    21961 gtagtccacg gcgcgccaaa gcgggtgaaa tactgcgcca gcgtcggcgg caggtagctg
    22021 gggttaaaac gccagctgtt atcctcagca aaacccactt tgcccggtaa caacatggaa
    22081 cccagcccag gcaccgtcac cacttcctcc cgcgcaatac gttttagcaa cgcgctgccg
    22141 atgtcggtat aacgctgctc tttccacaaa cgccccgcct ccagcaacga ccaggccatc
    22201 cagacatcac catcggaggc cgaattgctg tccagcactt cccacttact gttctctttc
    22261 ttgccccaca gccaggcggg caaacgttct tttaaagaac cctgagcgag attgttctgc
    22321 gtccagtcga gaatattatc gaaagctgca cggtcgttag ccgccagggc aaagaacatg
    22381 ccgtaacttt gcccttcaga ggtggtgatt ttgcgcgcgt cgctggggtc gatgacgcgc
    22441 ccttcctgac tgatgtaatc ctttttaaac tgctcccagg caggccaggt acaggttgcc
    22501 tgaacactaa aggcagccaa cagcagcatc gtcacgagtc cactacgcaa cacattcatc
    22561 ttcaattact cgttatccgg gttaagacga cgacggctga taatacgcag caaacgccac
    22621 agtacccatg ccagcaatat cacactgata gccgccagca ccgccagcag aatcggatgg
    22681 tttgccagcg cataccacaa gcgctcgaac cacggcagat gacctacgta ataaacgtcg
    22741 ccaacacgta ggctgttgat accggactcg cggatcaccg cgaccgaacc gaacatggtg
    22801 gcgcgtttgc cgctatcgtt caccacatcg ttaagcattt cataaccgcg tgggctatct
    22861 gccagcagcg caatcacgct gcgctggtcg ttatacggcg actggaagcc aatcaccgcc
    22921 gccatcgcac cggaagaggt cagcgttgac tgggtttctg ccgcgcgatc gctctcgtcc
    22981 ggcacaatgc cggggaacgg ggtctggcgc atcggtgttt tcacccagct ttcggtcgcc
    23041 tgcaccaata ggtcgatctg cttatcgtct ttcagtttgt ccgggatacc accgatgatc
    23101 atgatgtcgg catctttgcc ctgaatggtg ctgccatcat cggtcaccgt cagattaatc
    23161 gccgggaagc ccgtctgtgc gccgataaaa ccaacagtat tcagcaacgt ttccatctgt
    23221 gcttcgttag gcgttttcgg catcacggtg atggtttgcg acagatccgc catccggctg
    23281 aacgggaagc ccgcgttagc aaaggcgcgt agatccggca tcgggatgaa gtggtaatac
    23341 ttcgagaagt cgatggtgga gtcgtcacca atcaccacat gattctgcac cggctggaag
    23401 gtaatacagt tatccaccga accgcccggc atcgggttca tatactcaaa gtcgaagcgc
    23461 agctggttgg tcgcgcccag tttcagcgcc ggaatagaga catccgtttt gccatccagt
    23521 aaaccttgta ataccggaat ccgcagcagc aggcggttcg cctcctgttt gctgctcagg
    23581 ttgaaggatt gcaggaactg gttattcagg ctgatatcca tccgcgaact gtctttcacc
    23641 ggcggcatgg tgtagcggta attaatatcc atatcaatgc cggtactgcg catcaggtag
    23701 agatctggcg gcaggtttag cgaaacatta atcgctgctg gctcaagacc gctggattgt
    23761 aactgttctt cataggtttt cagttcacca aaggtgaccg gacgatcggt acgcacccag
    23821 ttcggcgcat cgtacggctt acgcggtagc agcggtttca cttcattcac taccacgctt
    23881 tcaccacgga acaggatgtt accctgagcg atacctttcg ctgcctgcaa caggtctttg
    23941 tcgtcacgac caaacaccac cagcagtttg acgtaaggat tctgcggatg gttgatcatc
    24001 tcaatcaccg gggcttttac cgccggatga tcgcgcagga agtccggccg tttgtcgttg
    24061 gttgcaaaga caatggcatt acgatccggc agttggttat agagcaccgg gaagttctgc
    24121 ccacgccagc cagaacgcga accaaaccac gaggcgacaa tggcagatgc ttgttgcagc
    24181 ccaacatccg gcgcacccgc aaagaccatc ggcaaggtgt tagtacggtt atcgcgcggg
    24241 tcaaagaatg gtaccgggaa gtgtgacagg tcattcttca cattcagggt ctgataggtc
    24301 aaatccagtc cactgctccg cccaacatcc agccaaagcg tggtgctggc cgggttttcg
    24361 cacacgtcct gataatggcc gacaaactcc agccgcacac ggttgaagtc ggtaataaac
    24421 agtgggttaa tgggcatttg cgccagcgtt tttttaccca actgttcttt ggtcactggc
    24481 agcacgccca tcagctcatc attgagataa acctttaact gcgactggac aggcagtaac
    24541 gatggcgatg gcgtgtattc gaggttgagc atcgccttcg tcaccacttc atcgctgcgc
    24601 ataccaaact caatgctgcc gttcgggtta atgccacgta gcaccatgct gcccggcggc
    24661 ggcgcaattt gcgcaaaggt cagcttcaca tcacgcgaag gaccgttctg cgccacgact
    24721 ggcgcatcag cgccctgcac gcccggcatc acttgcccca cctgcggatt ttgttccgtc
    24781 tgggcggcta tagctggctc agcattgatc agtggttgcg ttgctggcgt cgcctgcgtc
    24841 atgaaagagg ggaacgcact catccccata gccactgcac aaatccagaa tagttttctt
    24901 ttcatcgcgt tatcatcatt gttgagccaa agcctgatcc gatggttgtg ccgtttcgct
    24961 ccgctccggg cggcgcggaa taaacgatac aacccaggaa accagagaag tcagcacacg
    25021 gaatatgccc ttcaccgaag aaggcgcaaa ctccgccaga tggcggtagc cacggaagcc
    25081 gagcttcaga atatccagca gactttccag cggcttatct tccggatagc tgtcctgcca
    25141 gagcgcccat gtatccgcac gggcaaacgt acactgcaca aaatcgatat gttgctgggt
    25201 ggtgagcggc attaatttca gcccaacttc attacccatc acgcgcgcca cctgggtcgg
    25261 gaagacgtat tcctgctgac cgcgtttaag caacagattc actttctgcc cttccagaat
    25321 ctgcgcctga ccgttgatct tgatccccaa accaccgtcg gagaaatcct gaacggtaca
    25381 cgagaagagg tgaccatctt cgcgggcaat tgccgcgggc atcgtcatct ccacgcggtg
    25441 cgatcggcgt acctgtttgc tttctaccga taccgcaact gcgccgccaa gaacaatcag
    25501 gttgtagaat acccacacca tactgacgac cacggtgagc atctcggttg gcgggccata
    25561 gaagtagcgc cagataccta ccgcaacgcc caccaggttg agcagaacaa ggaagatgta
    25621 gggccgcgag atcacccagt cgacgtactc ttcttccacc agtccacctt tggcggtgac
    25681 gttaaatttg cctttgtgcg ggttaatcag cgccaccagc gtcggtggtg cgatatacca
    25741 cgccagcacc gtttcgtaga tttcactcca gaaagagtgg cgatatttgc cctggatctt
    25801 ggagttggtc aggctggcat ggatcatatg cggcagcacg aacagggcga tcatcaacgc
    25861 tggcgcatag atgatgtagg catgaagcag caggaacgcc agcggcgcag tcaggaagat
    25921 cagccgtgga atgcccgaca agaagtggaa catggcgttg acgtaacata gccgctgagc
    25981 aaacttcagc cctttaccgg tgagcgggtt atcgagacgg aagatttgca ccatcccgcg
    26041 cgcccagcga atacgctgac cgatatgcgc cgacagactt tcggtcgcca gccccgccgc
    26101 ctgcggaata cgcatatacg cggaggtata gccacgacgg tgcaggcgca aagaggtatg
    26161 cgcatcttca gtcacggttt cgacagcaat gccgccaatt tcatccagcg gcttacggcg
    26221 aatcaccgca caggaaccgc agaagaaagt ggcatcccac atatcgttgc cgtcctgcac
    26281 cagaccataa aacagcgtgc cttcgttcgg cgttttacga aaacgcccca ggttgcgttc
    26341 aaacgggtcc ggcgagaaga agtggtgcgg cgtctgcatc atcgccagct gtttctcttt
    26401 caggaaccag cccatggtca tttgcaggaa cgatcgcgtt ggtacgtggt cgcagtcgaa
    26461 aatcgacacg aactcgcctt tggcatattt cagcgcattg ttgatgttgc ccgctttcgc
    26521 atgttcatga gtggtgcggg cgatatattt cacccccacg ttttgcgcaa actggcgaaa
    26581 ctcttccctg ccgccgtcat cgaggatcca gatgttcagt ttgtctttcg gccagtcgat
    26641 acccagcgag gcgtaaatgg tatttttcac cacgttgaga tcttcgttgt aagtcgggac
    26701 aaagatatcc accgacggcc acagcgacat atctttcggc aatggcaccg gctgacgatt
    26761 cagcggccat actacctgga agtagccgag caccagcaca atccacgcgt acgtttcagc
    26821 gaagagcaga ataagcccgc acaccaggct gaccggatcg tcccagttca gcgtagaggt
    26881 gtaacgccac cagatataac ggcaagaaac ggtcagcgac agcacaatca acattagcgc
    26941 cgagaagcgc cccggcatcc gccgtacgat cagcgctacc ccccacagca gcatcaggaa
    27001 gataaactgc gccagcgggt taaacggctg agtaacgcag attaacgcca gaatcagcga
    27061 gaaggtgacg atgataccga ggatcaaccg ccgcgccccg gcactcaaat gaccgagctc
    27121 ttttttctca tcaagatgct gtgttttatg gctaacgcgc tcaggcagct cgttcatcca
    27181 ttgatggtaa cgtccacgaa tattctgcag acctgaaaat gcccgcctgc gcggtttcgg
    27241 cgtttctttg cgcgatgcac cgatcagtaa ccagcatgtt tgaatgagat aacggaccgg
    27301 gtccagcgga cgcggacgcg aggcgttgat atgcggatac aggtttttat gttctgcgcg
    27361 aatacgctgc cagcgcgggt gctccagcgg aataaaaatc caggccagga tcatccagaa
    27421 acagccgagc gtcgcgctga aagccgacgc accgtgacga cgataatcgc gataacgccc
    27481 gataagccgc gcgttgaccg gcgggataag caaccaccgg gtcaggatac tcatgatgca
    27541 ctcccgactg gcgttttcag cccggaatag ttcaacaggc accagttcgc cagcgtcaat
    27601 atctcttcag ccgccagcgc atcactgcga tattcaccta ctggttgctt agccgccagg
    27661 cattcagcca tcgcttcatc acgatgaatg agcatcggca gtaatcggcg ctggctttgc
    27721 aaccaaagct ggtaaatatc gtcctgaacc tgactgccaa tacggaagtc attaatcaaa
    27781 atatgtgcgc catccggcag cgcttgctga tgcagtcgga tatggcagtt ggcatccaca
    27841 ttgacgattg ccagcgagtg atcgcacaaa ctcagcagct ggtgggttat ctgcgaggca
    27901 tcacgcggta agtcgattaa aatccactgg taacgcccgc tggcttttag ttgctgtaag
    27961 ccggagcaaa tatcgctcag ccgggtttgc cagtgctgtg gattttcttg ttcttcaatg
    28021 gataactgac caaaaggtag caaatcgagc tgcgaggtgt agcgcaaccc agcgtcacgc
    28081 cagtcctggc catccagcat agctctggcc cagccctgac ggtgggtaaa atcaacgtta
    28141 aatgacaggc gcaacaagtt gtccgggcag gcatcgacca ccaggacatt ttctcccaac
    28201 atttgtaatg accaggctaa tgcggcggtg atggttgttg tccccacgcc tccccgcacc
    28261 ccctgcaatc ccagtacggc cattcaccaa tcccctactt ttgttgcgca aactctgcca
    28321 gcaacggcca gcgttttaat gccgcggcca actgttcgcg ttgggaaata tcggcataat
    28381 caatatcagg cagtgaaaat gcctgcttta acgccaaaat atcattctgg aagatatagc
    28441 ctatcgcggg atcaggcaga gtatctggtt cattgttatt cattttgctt gatccctatg
    28501 aaataaccgt accgttcatg agacaattct aaccgtgagc atttcgccgg tttattgttt
    28561 acggcgagac gggaatttgc tcactttttg actttccgtt acgcaacaga aaaagttaaa
    28621 aacgaaaaga ctaaaatcac acgtcttgtg acctgctaaa ttgattcaca tgctaaatct
    28681 gataagtttt aatttcaatg gtaggtttat ttcttagctt tcgctagtaa actgataatc
    28741 agacaaaatg gtgacatgag ggacattgtg gaccctgtat tctctatcgg tatctcatca
    28801 ttatgggatg agctgcgaca tatgccagca ggcggcgtct ggtggtttaa cgtcgatcgc
    28861 catgaagatg ctatcagtct ggcgaatcaa acaattgcat cccaggctga aaccgcacac
    28921 gtcgcggtca ttagcatgga cagcgatccg gcgaaaatct ttcaattaga tgattctcaa
    28981 gggccggaaa aaataaaatt attttcaatg ctaaatcatg aaaaaggtct atactatttg
    29041 gcccgtgatt tgcagtgttc tattgatccc cataattacc tttttattct tgtttgcgca
    29101 aataacgcat ggcaaaacat tcctgccgag cggcttcgct catggttgga taaaatgaat
    29161 aaatggagca ggttaaacca ttgttcgctt ttggtaatta atcccggaaa taataacgat
    29221 aaacaatttt cattgttgct tgaggaatac cgttcacttt ttggtcttgc cagtttgcgt
    29281 tttcagggcg accaacattt gctggatatt gccttctggt gcaacgaaaa aggggtcagc
    29341 gcccgtcagc agcttagcgt tcagcaacaa aatggtatct ggacattagt tcaaagcgaa
    29401 gaggcggaga tccaaccacg cagcgacgaa aaacgcattc tgagtaatgt tgctgtactg
    29461 gaaggtgcgc cgccgctatc ggaacactgg caactgttca acaataacga agtcctgttc
    29521 aatgaagccc gtaccgctca ggcggcgacg gtggtctttt ctttacaaca aaatgcgcaa
    29581 atcgagccac tggcccgcag cattcatact ctgcgtcgcc agcgcggtag tgcgatgaaa
    29641 atcctcgtac gggaaaatac cgctagcctg cgcgccaccg atgaacgttt gttattggcc
    29701 tgcggtgcaa atatggttat cccgtggaat gcgccactct cccgctgtct gacgatgatc
    29761 gaaagcgtgc aagggcagaa gtttagtcgc tatgtgccgg aagatatcac taccttgctg
    29821 tcaatgaccc agccgctcaa actgcgtggt ttccagaagt gggatgtgtt ctgtaatgcc
    29881 gtcaacaaca tgatgaataa ccctctatta cctgcccacg gtaaaggcgt tctggttgcc
    29941 ctacgtccgg taccgggtat ccgcgttgaa caagccctga cgctgtgtcg ccctaaccgt
    30001 accggcgata tcatgaccat tggcggtaat cggctggtgc tgtttctctc attctgtcgg
    30061 attaacgatc tggataccgc gttgaatcat attttcccat tgcctactgg cgacattttc
    30121 tcaaaccgta tggtctggtt tgaagatgat caaatcagtg ccgagctggt gcagatgcgc
    30181 ctgcttgccc cagaacaatg gggcatgccg ctgcctttaa cacaaagttc taaaccggtc
    30241 atcaatgccg agcacgatgg tcgccactgg cgacgaatac cagaacccat gcgactgtta
    30301 gatgatgctg tggagcgctc atcatgatga ccatcagcga tatcattgaa attattgtcg
    30361 tttgcgcact gatatttttc ccgctgggct atctggcgcg gcactctttg cgacgcattc
    30421 gcgacacctt acgtttgttc tttgctaaac ctcgttatgt taaaccggct gggacgttac
    30481 gccgcacgga aaaagccagg gcaaccaaaa aatgactcaa tttacgcaaa ataccgccat
    30541 gccttcttcc ctctggcaat actggcgcgg cctttccggc tggaacttct attttctggt
    30601 taagttcggc ctgttgtggg cgggatatct taacttccat ccgctcctca atttggtgtt
    30661 tgccgcgttt ctgctgatgc ccattccgcg ctacagcctg catcgcttgc gccactggat
    30721 tgccctgccg atcggctttg ctttgttctg gcatgacacc tggttgcctg gcccggaaag
    30781 cataatgagc cagggttcgc aggtggcggg gttcagtacc gattatttaa tcgaccttgt
    30841 cacacgcttt attaactggc agatgattgg ggccattttt gttttattag tggcctggtt
    30901 attcttgtca caatggattc gcattaccgt ttttgtggtt gccatactgc tatggctgaa
    30961 catacttacc ctggcgggac caagtttctc cttgtggcca gccggtcaac cgacgaccac
    31021 tgtaacaacg acgggtggta acgcagcggc aaccgttgcg gcgacgggtg gcgcaccggt
    31081 agtgggtgat atgcccgcac aaactgcacc gccaacaacg gcgaacctta acgcctggct
    31141 gaataatttc tataacgcgg aggcgaaacg taaatcgatc ttcccgtctt cgctgcccgc
    31201 tgatgctcag ccatttgaac tactggtgat taatatctgt tcgctttcct ggtcggatat
    31261 agaagccgcc gggttgatgt cgcatccgct gtggtcgcat ttcgatattg agttcaagaa
    31321 ctttaactcc gccacctcct acagtggccc ggcagcgatc cgtttactgc gcgccagctg
    31381 cgggcagact tcgcacacta atctgtatca accggcaaat aacgactgct atctgtttga
    31441 taacctgtcg aaactgggct ttacccagca cctgatgatg ggacataacg gccagttcgg
    31501 cggttttttg aaagaagttc gcgaaaatgg cggcatgcag actgaattga tggatcaaac
    31561 aaatctgccg gttattttgc tgggctttga tggttcgccg gtttatgacg ataccgccgt
    31621 gcttaaccgc tggctggacg ttaccgaaaa agataaaaac agccgtagtg ccacgttcta
    31681 caacacgctt ccactgcatg acggcaacca ttatccgggc gtcagcaaaa cagcggatta
    31741 caaagcgcgg gcgcagaaat tctttgatga actggacgcc ttctttactg aactggagaa
    31801 atcgggtcgt aaagtgatgg tggtcgtggt gccggaacac ggcggcgcgc tgaagggcga
    31861 cagaatgcag gtatctggcc tacgtgatat ccctagcccg tctatcaccg acgtccccgt
    31921 tggggtgaaa ttcttcggca tgaaggcacc acatcagggg gcaccgattg tcatcgacca
    31981 accgagcagc ttcctggcta tctccgatct ggtggttcgc gttcttgatg gcaagatttt
    32041 caccgaagat aatgttgact ggaaaaaact caccagtggg ttgccacaaa cagcaccggt
    32101 ctccgagaac tcaaatgcag tagttattca ataccaggat aaaccgtacg ttcgcctgaa
    32161 cggcggcgac tgggtgcctt acccgcagta agcacgaaaa aggccgcaga gtttcacccc
    32221 tgcggcctgg tccgggcgca aattgccatt acggcagcct gacgcccgca tgacacgtta
    32281 cttccgctta ttcagccagt tcacgatcat actggcaaga atgccagcaa tgaccggagc
    32341 cgctaaatca tgccagaagg ccatgcccag ttctgcgagc gtcatatagc cgcctgtgtt
    32401 gtaatgacaa cgtttcgcgg ctattcttga gttgtttggg ttcaagatta gcccccgtga
    32461 tgttgtcagg tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac
    32521 cagggataaa gcccccgcaa cattgcgcct caccggaacc tttccggctt gccgctgatt
    32581 ctacgactgt tt
//
LOCUS       DAPGRN010000048        32421 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPGRN010000048 DAPGRN010000000
VERSION     DAPGRN010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.048"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..1123)
                     /gene="fabY"
                     /locus_tag="RHW33_002378"
                     /gene_synonym="yiiD"
     CDS             complement(182..1123)
                     /gene="fabY"
                     /locus_tag="RHW33_002378"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV1645874.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1168..1605)
                     /gene="dtd"
                     /locus_tag="RHW33_002379"
     CDS             complement(1168..1605)
                     /gene="dtd"
                     /locus_tag="RHW33_002379"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV1645875.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1602..2474)
                     /locus_tag="RHW33_002380"
     CDS             complement(1602..2474)
                     /locus_tag="RHW33_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV1645876.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2468..3067)
                     /gene="yihX"
                     /locus_tag="RHW33_002381"
     CDS             complement(2468..3067)
                     /gene="yihX"
                     /locus_tag="RHW33_002381"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV1645877.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGTWSDLTRIPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3166..3951)
                     /locus_tag="RHW33_002382"
     CDS             complement(3166..3951)
                     /locus_tag="RHW33_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1645878.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3985..4881)
                     /gene="yihV"
                     /locus_tag="RHW33_002383"
     CDS             complement(3985..4881)
                     /gene="yihV"
                     /locus_tag="RHW33_002383"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HDV1645879.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAT
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5049..5945
                     /gene="yihU"
                     /locus_tag="RHW33_002384"
     CDS             5049..5945
                     /gene="yihU"
                     /locus_tag="RHW33_002384"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV1645880.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5969..6847
                     /gene="yihT"
                     /locus_tag="RHW33_002385"
     CDS             5969..6847
                     /gene="yihT"
                     /locus_tag="RHW33_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HDV1645881.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6863..8104
                     /gene="yihS"
                     /locus_tag="RHW33_002386"
     CDS             6863..8104
                     /gene="yihS"
                     /locus_tag="RHW33_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HDV1645882.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            8171..9073
                     /locus_tag="RHW33_002387"
     CDS             8171..9073
                     /locus_tag="RHW33_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HDV1645883.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVTSPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCSDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GLDLLLLEPGKTHRLFFNIYGQHN"
     gene            9134..10009
                     /locus_tag="RHW33_002388"
     CDS             9134..10009
                     /locus_tag="RHW33_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV1645884.1"
                     /translation="MDFFTLFSPGGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLV
                     FGKKVAAIRQAEASK"
     gene            10117..12153
                     /gene="yihQ"
                     /locus_tag="RHW33_002389"
     CDS             10117..12153
                     /gene="yihQ"
                     /locus_tag="RHW33_002389"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HDV1645885.1"
                     /translation="MDTPRPQLPDFEFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDVAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGETFHGGEITVEAPIGKPPVFYRADSEWAALFASLKNI"
     gene            12199..13584
                     /locus_tag="RHW33_002390"
     CDS             12199..13584
                     /locus_tag="RHW33_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645886.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFIGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVNF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            13624..15027
                     /locus_tag="RHW33_002391"
     CDS             13624..15027
                     /locus_tag="RHW33_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645887.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILIGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            15094..15786
                     /gene="ompL"
                     /locus_tag="RHW33_002392"
     CDS             15094..15786
                     /gene="ompL"
                     /locus_tag="RHW33_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HDV1645888.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(15877..17142)
                     /locus_tag="RHW33_002393"
     CDS             complement(15877..17142)
                     /locus_tag="RHW33_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645889.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(17244..18224)
                     /locus_tag="RHW33_002394"
     CDS             complement(17244..18224)
                     /locus_tag="RHW33_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV1645890.1"
                     /translation="MMTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDCGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(18232..18942)
                     /locus_tag="RHW33_002395"
     CDS             complement(18232..18942)
                     /locus_tag="RHW33_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV1645891.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(19158..20981)
                     /gene="typA"
                     /locus_tag="RHW33_002396"
     CDS             complement(19158..20981)
                     /gene="typA"
                     /locus_tag="RHW33_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV1645892.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            21354..22763
                     /gene="glnA"
                     /locus_tag="RHW33_002397"
     CDS             21354..22763
                     /gene="glnA"
                     /locus_tag="RHW33_002397"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV1645893.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22937..23986
                     /gene="glnL"
                     /locus_tag="RHW33_002398"
     CDS             22937..23986
                     /gene="glnL"
                     /locus_tag="RHW33_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV1645894.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23998..25407
                     /gene="glnG"
                     /locus_tag="RHW33_002399"
     CDS             23998..25407
                     /gene="glnG"
                     /locus_tag="RHW33_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV1645895.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            25519..25629
                     /locus_tag="RHW33_002400"
     CDS             25519..25629
                     /locus_tag="RHW33_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV1645896.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(25818..27191)
                     /gene="hemN"
                     /locus_tag="RHW33_002401"
     CDS             complement(25818..27191)
                     /gene="hemN"
                     /locus_tag="RHW33_002401"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV1645897.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLHENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(27380..27886)
                     /gene="yihI"
                     /locus_tag="RHW33_002402"
     CDS             complement(27380..27886)
                     /gene="yihI"
                     /locus_tag="RHW33_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV1645898.1"
                     /translation="MKPSSPSRSKGHAKARRKTREELNQEARDRKRQKKRRGHAPGSR
                     AAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELLE
                     TDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQED
                     MMRLLRGN"
     gene            28468..29100
                     /locus_tag="RHW33_002403"
     CDS             28468..29100
                     /locus_tag="RHW33_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV1645899.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFNEMQPVEETQDGE"
     gene            complement(29482..32268)
                     /gene="polA"
                     /locus_tag="RHW33_002404"
     CDS             complement(29482..32268)
                     /gene="polA"
                     /locus_tag="RHW33_002404"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV1645900.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
BASE COUNT         7857 a         8347 c         8033 g         8184 t
ORIGIN      
        1 gataattgcc atacagctct acaaataata tttaaatgct cctgaacagt gatgtagcaa
       61 gacacgtatg gagccaacga agaataaaaa tttatagagt tcttattcaa tataaccgaa
      121 atagccaagg actaatataa atgacctgaa tgagagaggc agcaccgccc ctctccatca
      181 gctactcttc ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg ggcagaacga
      241 tatacgtgcc ttcaaacact gcacccggcg tctcgtcgcc aaagatttcg acctgcatct
      301 gcacccgtgc ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag gcaccaaggt
      361 cggctaccgc atggggttta ccgctaatcg gtttgctgta gcggatatgc gcatccgcca
      421 gaataatcgt tccgccgagg tggcgttcac gcagcatcag ccagataagc ccccaaccgg
      481 tgagcgtcgc cagtgagaat aaactcccgg caaacagcgt atggtgcgga ttctgattgc
      541 cggtttctgg catggtggtg ataaattttt gcccggtata ttgttgaatg cgcacgccca
      601 ttttttcgct taacgggata tgttcgtacc acgcctgttg cagctgcgcg caccagtcac
      661 cgcgatgcag aatgtcatcc agagtggcaa cgggtttaat catcaaaaaa tggcgaatcg
      721 gcgtggtagt tggggtggtg atctccccct gattaatgaa tcccagcttg gcgaaaaact
      781 ccaccgcgtc ttcacgggcg ctacaggtca cgcgcttaac gccttcctga cgcgccaccg
      841 actccagggt catcgccatc agcgtgccta accctttgtc ctgcacgtcg ggatgaacgg
      901 ccataaagcg aatggacgct tcattgtcgg cattaatata cagtcggcct accgccacca
      961 gattaccctg ctcgtcgacg accatctggt gatgcgccat cgcatcccac gcgtcgcgtt
     1021 ccgaaccttt tggttgatgc aggggcttac gcaacatttc ccagcgaaac tgatagtaac
     1081 gctctaattc ttcttctgtt tgtggaaccc gaaggtgata catagcgata ctctctcttg
     1141 tttcccgtga caaccctgga agctggctca tacctgcaac cagaatgtca cggggccatc
     1201 attgaccagc gatacctgca tatccgcagc gaagcgtcct gtttgcgtgt tcatctcttg
     1261 ctgacggcag cgttcgacga aatagtcata taacgcctct gcgcgatccg gtgatgcacc
     1321 tttggagaaa cttggacgca tcccccgttc ggtatctgcg gcgagggtaa actgggaaac
     1381 taccagcaca ctgccgcccg cctgttgcac gttgagattc atcttgcctt cggcatcgct
     1441 aaagatgcgg tagccgagca cacgctcgca cagacggttt gctttctgtt cgtcgtcatc
     1501 cttttcgaca cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca cttctccctc
     1561 cacggtgacg ctggcacggg ttacgcgttg aattaatgca atcatggttc gtcgtcttct
     1621 tcttgttcag ctgcttgttt tagtttgcgg tattccccga gagtgacagt aatttccgcg
     1681 ccaagcaaga cgatacacca tgtccagtag acccaaacaa agagaatggg gatcaccgcc
     1741 agcacaccgt aaatgagctg atatgacggg aacatggtga tataaagcgc gaaacctttc
     1801 tttcctgctt cgaacaggag cgcggcgaca aacgcgccga caatcgcgtc gcggttaggt
     1861 acgcggatgg taggaacaat gctgtacagc aaccagaagg agatccacga caacagcagc
     1921 ggaaaaatac gcagcacatt gtcgatgaca gtattgagat cgctcgccca gcgcagggag
     1981 agcaaatagg aactgatcgc cagactggcc cctgccagca gcggccccag cgttaaaatc
     2041 atccagtaca cggcgaacga gtaaattttt ggtcgagctc gtttactgcg ccagatggta
     2101 ttcaacgcgc tatcgatgga gtacatcaac aataacgcgg tgacgatcag cccgcacgcg
     2161 ccaacggcgg tcatcttgtt ggaattggca acaaattgtt cgatataccg ctgaataaca
     2221 tcgccagtag caggcagaaa gttggcaaaa ataaagtgac gcaactgaat gctgacgtcg
     2281 gaaaacatgg gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg cactaatgag
     2341 agcaacgaca cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatgcgt
     2401 tgccagagta gttttagcca ggcccatagc ggacgggtac ggtgcctggc tttgtcctga
     2461 atggttttta gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca
     2521 gaatactggt aatgcccagc tgattagctc cttctatatt atcggcgtta tcgtcgaaaa
     2581 agaccgtatc gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgagctt
     2641 caggtttgcg catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt
     2701 ccgggtattc ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca
     2761 cgcgatgccc ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa
     2821 caaacacagc ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat
     2881 gacacagcgc ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg
     2941 cctcccccat atgaaaactc ttcttaagcg tagccaacgg aatacgcgtt aaatcgctcc
     3001 aggttcccag cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat
     3061 agagcatttt tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt
     3121 tggctatgtg gcaaggtatg aagccaggtc aacggtcaca aaaatctacg cgtcttcctg
     3181 gggaagaatg atttcaattt ggctgtcttg taagcgtgat tttagcgcag cgggcggcag
     3241 ctcgtcggta aagagcgcgg tgacctgtgc cacgttacca atttcaaccg ccgccgaagc
     3301 atgatactta gtgtgatcgg cgaccagcag gatatttctc gcgtgcgcca tcatcgtttt
     3361 caccacgtta gcttcgttaa catcaaactc catcaacgcg ccatcgctct cgattgcgcc
     3421 tacgcttgtt accagataat cagcacgaaa atcggccaca aaggacgccg ctgaagggcc
     3481 aatgatcccg ctattatgag agcgcaacgt accgccgggc accatcactt caaagcgcgg
     3541 gttgtggtaa agaatatgcg ccacacgcag gctgttggtg attatccgca aatgattatg
     3601 gttaagtaac gcccgggcga catgctcaac ggtcgtacca atggtgataa atattgttga
     3661 accatcagga atatagtctg ccaccgcttc ggcaatcgct tttttttcct cggtttgcga
     3721 aacctcacgc tgctcgaacg ccgtattaac gacgctggaa gcccgacccg cgccaccgtg
     3781 atggcgcgta atcaggcctt gctcgcttaa tttacgaata tcccggcgca ctgtctgcgt
     3841 ggagacatcc agcagatttg ccagctcatc aatattcata tacccacgct cggcgatcag
     3901 catgaggagt tggtcgtgcc gcgggttacc ggttagttcg gtaaggctca tgaaaattcc
     3961 tcgaaaaacc atcaccctgg cattttatac aaaaagtgac aaaaaagatc gggtttgatc
     4021 acagtcaggg atcccggctc gtccaccggg acgcgtgcat ttcaacgccg ctacaccgct
     4081 ggcgaagcgg actgactccg ctaaatcccc acctgttgcc agcgccaccg ccaatgcgcc
     4141 gtgaaaaaca tcacccgcac ctgtggtatc taccacatca actttgaagg ccggttgatg
     4201 ttgacgcccg ccattttcca gccagtcaca gcctgcgctg ccctgggtca catagacatg
     4261 accatttgtg agcgtttgtg cctgttttag cgcactggcc atctctttca cgcccgttaa
     4321 gcgcgccaga cccggttctg aaaaggccgc atgatcgctt aatgccacca gctcgctgat
     4381 atcctgcggc gtaatatcac cgtccagaac ggtcatcacg cccgcctgcc gggccagagt
     4441 gaaggctttt ttagcgccgt cgtgccagcg tacatctgcc agcacaacat cccactgaga
     4501 gaaatcaatt tcctccaacc actctgcgtc aggcagcagg tccgggctgg ggtagttaat
     4561 gattatccgc tcgcctgtgg catccaccat gatggcggat tgcgatgatt tcgcctggtt
     4621 ataccgtttg gtgtaacggg tgttaacgcc ccaggattcc agctctgcca gcaggctgtt
     4681 gcccgtgtcg tcatcaccta cgcgaccaat aaaatcaacc tgcgccccca gccttgccgc
     4741 cgcaaccgct gccgtcgccg ctggcccgcc accaacttcc gtataatttc tcgccacgta
     4801 tttaccgctc tccgtcggta acccttccac gtaatagatg cgatccatca cggttatacc
     4861 tacacaagca acacgaatca tggtcattcc ttaaacattt tgggatgcgt tcattttaat
     4921 ttcaccaatt gtttaaaaag tgacggctgt caattttttg accataaatt acaaatacga
     4981 tcaaaaacag acaatcaaag cgtgtcataa atgacaaaaa gtgacatcac tgtattcagg
     5041 agaaggttat ggcagcaatc gcgtttatcg gtttaggaca aatgggttcg ccaatggcga
     5101 gcaatttatt gcagcaaggg caccaacttc gcgtctttga tgtgaatgcc gaggctgtgc
     5161 ggcatctggt agacaaaggc gcgactcccg ccgccaaccc ggcgcaggcc gctaaagatg
     5221 ccgaatttat cattaccatg ttgccgaatg gcgatctggt gcgcagcgtg ttgttcggtg
     5281 aaaacggcgt ttgcgaaagc ttatctaccg atgcgctggt cattgatatg tcgaccatcc
     5341 atccgctgca aaccgataaa ttgattgccg atatgcaagc caaaggcttc agcatgatgg
     5401 atgttccggt aggccgtact tctgcaaatg ccattaccgg tactctgtta ctgctggctg
     5461 gcggcaccgc tgaacaagtt gaacgtgcca cgccgatcct gatggcgatg ggcagtgagt
     5521 tgatcaacgc gggcggtccg ggcatgggga tccgcgttaa actcatcaac aactatatga
     5581 gcatcgcgct caatgcgctt tcggcagaag ccgccgtttt gtgcgaagcc ctgaatcttc
     5641 ccttcgatgt tgccgtcaaa gtgatgagcg gtaccgccgc cggtaaaggc cacttcacca
     5701 cttcctggcc aaacaaagtc ctcagcggag atctttctcc cgccttcatg atcgatcttg
     5761 cccataagga tcttggcatc gcccttgatg tcgccaacca gctgcatgtg ccaatgccgc
     5821 tgggggccgc ctcacgggag gtttatagcc aggcgcgcgc agcaggtcgc ggtcgccagg
     5881 actggtccgc cattctggaa caggtccgtg tcagtgccgg gatgactgcc aaagtaaaaa
     5941 tgtaacgact ggataaagga ataaatgaat gaataagtac accatcaacg acattacgcg
     6001 cgcatcgggc ggttttgcca tgctggcggt cgatcagcgc gaagccatgc gcatgatgtt
     6061 tgccgcggct ggggcacccg ctccggtagc cgatagcgtt ttaactgatt tcaaagttaa
     6121 cgcggcgaag accctctcgc cttatgcctc ggcgattctg gtagatcaac aattctgtta
     6181 ccgccaggtg gttgagcaaa acgcgattgc caaaagttgc gccatgattg tcgccgccga
     6241 tgagttcatt cctggcaacg gtattccggt agatagcgtg gtcattgacc gcaaaatcaa
     6301 tccgctacag atcaaacaag acggtggcaa agccttaaaa ctgctggtgc tgtggcgtag
     6361 tgatgaagat gcgcagcaac gcctggatat ggtgaaagag tttaacgaac tgtgccactc
     6421 acacggcctg gtaagcatta ttgagccagt cgtccgccca ccacgtcgtg gcgataaatt
     6481 cgatcgcgaa caagcgatca tcgatgccgc caaagagctg ggcgacagtg gcgctgacct
     6541 ctacaaagtt gaaatgccgc tttacggtaa aggtccgcaa caagagcttc tcagtgcttc
     6601 acaacgtctg aatgaccata tcaatatgcc gtgggtgatc ctctcttccg gtgtcgacga
     6661 aaaactgttc ccgcgtgccg tacgcgtggc aatgacagcg ggtgcatcgg gcttcctggc
     6721 tggtcgtgct gtctgggcat cggtcgtcgg tttaccagac aacgagctga tgctgcgtga
     6781 cgtttgcgca ccgaaattac aacaactggg cgatatcgtc gacgaaatga tggctaaacg
     6841 ccgttaagat ggggatacaa aaatgaaatg gtttaacact ttaagtcaca accgttggct
     6901 ggaacaggaa accgatcgca tctttgattt tggtaaaaat tccgtagtac cgactggatt
     6961 tggctggtta ggcaataaag ggcaaatcaa agaagagatg ggcacccatc tgtggatcac
     7021 agctcggatg ttgcacgttt attccgttgc tgcggcgatg ggtcgacctg gcgcttactc
     7081 gttggttgat cacggtatta aagccatgaa cggcgcactg cgcgataaaa aatatggcgg
     7141 ctggtatgcc tgtgtgaatg acgagggcgt ggtggatgcc tccaaacagg gctatcaaca
     7201 tttctttgct ctgctgggtg ctgccagcgc cgtcactacc ggtcatccgg aagcgcgcaa
     7261 gctgctcgat tacaccattg aaattatcga gaaatacttc tggagcgaag aagagcagat
     7321 gtgcctggag tcctgggacg aagccttcag caaaaccgaa gagtaccgcg gcggcaatgc
     7381 caatatgcac gcagtggaag ctttcttaat tgtttatgac gtaacgcatg acaaaaaatg
     7441 gctggatcgc gcgattcgcg tggcttccgt gattatccac gacgtcgcaa gaaataatca
     7501 ttatcgcgtt aacgaacact tcgataccca gtggaatccg ctgccggatt acaacaaaga
     7561 taacccggcg caccgcttcc gcgcgttcgg aggtacacca ggccactgga tcgaatgggg
     7621 ccgtttaatg ctgcacatcc acgcggccct ggaagcccgt tgcgaacaac cgccagcatg
     7681 gctgctagaa gatgccaaag gtctgtttaa cgccaccgtg cgtgatgcct gggcacccga
     7741 tggtgcggac gggattgttt ataccgttga ctgggaagga aaaccggtgg tccgcgaacg
     7801 tgtgcgttgg cctatcgtcg aagcgatggg tacggcctac gcgctctaca ccgtcaccgg
     7861 cgatcgccag tatgaaacct ggtatcagac atggtgggac tactgcatta agtacctgat
     7921 ggactatgaa aatggttcct ggtggcagga gctggatgcg gacaataagg tcaccaccaa
     7981 agtctgggac ggcaaacagg atatttatca cctgctgcat tgcctggtga tcccgcgtat
     8041 cccgttagcg ccaggcctgg ctccggcagt tgcggcgggt ctgctggata ttaacgcgaa
     8101 ataactgact aaaaaaacga catatcaggc tgcacgccgg gtatgtcgtt attcaataaa
     8161 ggtgcctcgt atgcaaatca ccaacatgca ttgcagtgga cagaccgttt cacttgccgc
     8221 gggggattat cacgcgacca ttgtgaccgt cggtgcaggt ctggctgaat taacctttca
     8281 gggctgccat ttagttattc cacataaacc cgaagagatg ccgctggcac atctgggcaa
     8341 agtccttatt ccgtggccga accggatcgc caacggctgt tatcgctatc aggggcagga
     8401 atatcagtta cccattaatg aacatggttc aaaagccgct attcatgggc tgctcgcctg
     8461 gcgtgactgg caaatcagcg aattaaccgc caccagcgtt accctgacag cttttctgcc
     8521 acccagttac ggctatccgt ttatgctggc ttcgcaggtt gtttattcgc tcaatgcacg
     8581 cacgggtctt tccgttgaga tcgcaagtca gaatattggt actgtgacct caccctatgg
     8641 tgtgggtatt catccttatt taacctgcaa cttaacatct gttgatgaat atctgttcca
     8701 gcttccggca aaccaggttt atgccgtcga tgagcacgct aatccgacaa cgcttcacca
     8761 cgttgatgag ctggatttaa atttcacgca ggcgaaaaaa atagccgcga caaaaatcga
     8821 ccacaccttt aaaaccgcta atgatctgtg ggaaataacc attacccatc cacaacaggc
     8881 gctttcggta agcctttgtt ccgatcaacc ctgggtgcag atttatagcg gcgaaaaatt
     8941 acagcgtcag ggactggcag ttgaacctat gagttgccca cctaacgcct ttaattcagg
     9001 tctcgactta ttattactgg aaccagggaa aacgcaccgc ctgtttttta atatttacgg
     9061 gcagcacaat taattttatt aagaaaagaa cgagatcatt gtttagggtt gtatttacac
     9121 aacaggaaat atcatggact tttttacgtt attttcaccg ggtggggtca tgctggaaac
     9181 aaacgtggta tgtatcgtta tcgcgttaat gtttctttat acctttgtgg ggatatgcgc
     9241 gggatttggc ggtggtttaa ccactatgcc gctcgtcacg ttaatgctcc ccgtaaaaat
     9301 ggcaacacca ctctcggtaa ttgtcggcac cgcgaccgcc ttgtacgcca cctggctgtc
     9361 acgtaaagaa acggattggc gatcggcaat ggtactgatt attttctcgt ttctgggaat
     9421 acccgtcggt ttatatgcgc tgtcgtattt acccgaccat tttatgaaag tgggcttagg
     9481 cggattcctt atcctgtact ccttttacag tatgtttatt ccacgcctgc cgatttatga
     9541 ccgtagctgg atcgccgcac ctatcggtgc agtagcaggc gcattaggcg cagctttctc
     9601 aaccaacgga ccaccgatgg ttatctacgg tatgctgcgt aacttaggcc cggcggcatt
     9661 tcgtggcact ctgaacgctt tcttcaccgc aaacaacatc gcggtgatcg gcgggctggc
     9721 gaccagcgga attttgactg tttcgatctt taaactggtg ctgttttgta ttcccaccat
     9781 gatcctcggt tcactggttg gacaatatgt gcataaacgt atttcagtca aagtgttccg
     9841 tgtgatggtc tttttattgc tgattgcctc cggttccatg ttgataaaag gcgcgctggg
     9901 aattcccgcc gctgccgcac ttctacctcc gtttaccttg ttagttatac tgcaactggt
     9961 atttggtaaa aaagtggcgg caatccgtca ggctgaagca agtaaataat cacttatgcg
    10021 ggtttaaatt tatatccgca cacattcttt aattaattaa acctgtaata acaggatgac
    10081 atgaaatatt agctttatac tctatggaga ttatttatgg atacgccacg cccacaatta
    10141 ccagattttg aatttcatca gaataacgac agttttaccc tacattttca acaacgtctt
    10201 attttaaccc atagcaaaga taatccttgt ttatggattg gttcaggtat tgcggatatc
    10261 gatatgttcc gcggcaattt cagcattaaa gataaactac aggagaaaat tgcgcttacc
    10321 gacgccatcg tcagccagtc gccggatggt tggttaatcc atttcagccg tggttctgac
    10381 attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct ggaactacaa
    10441 aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat
    10501 atctacggct gcggcgaaca gttttcctac ttcgatctgc gtggcaaacc gttcccgcta
    10561 tggaccagtg aacaaggcgt tggtcgcaat aaacaaacct atgtcacctg gcaggccgac
    10621 tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc tacgtttgtc
    10681 agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt cgactttagt
    10741 gccccggaat accatgaact ggcgctgtgg gaagacaaag caacgctgcg ttttgaatgt
    10801 gctgacacat acatctccct gctggaaaaa ttaaccgccc tgctgggacg ccagccagaa
    10861 ctgcccgact ggatttatga cggagtaacg ctcggcattc agggcgggac ggaagtgtgc
    10921 cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat ctgggcgcag
    10981 gactggtccg gtattcgtat gacctctttt ggcaaacgcg tgatgtggaa ctggaagtgg
    11041 aacagcgaaa actacccgca actggattcg cgtatcaagc agtggaacaa agagggcgtg
    11101 cagttccttg cctatatcaa cccgtatgtt gccagtgata aagatctctg cgaagaggcg
    11161 gcaaagcgcg gttatctggc aaaagatgtc gccggtggcg actatctggt ggagtttggc
    11221 gagttctacg gcggcgttgt cgatctcacc aatccggaag cctacgcctg gttcaaggaa
    11281 gttatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga cttcggcgag
    11341 tatctgccca ccgacacgta cctgcataac ggcatcagcg cggaaattat gcataacgcc
    11401 tggcccgcgc tgtgggcgaa atgtaactac gaagcccttg aagaaacggg caagctcggc
    11461 gagatcctct tctttatgcg cgccggttct accggtagcc agaaatactc caccatgatg
    11521 tgggcgggtg accagaacgt ggactggagt ctggacgatg gcctggcgtc ggttgtcccg
    11581 gcggcgctat ctctggcaat gaccggacat ggcctgcatc acagcgacat tggcggttat
    11641 accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc
    11701 gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt
    11761 gacggcgacg cggaaaccat cgcccatttc gcccgtatga ccaccgtctt caccaccctg
    11821 aaaccttacc tgaaagaggc cgtcgcgctg aatgcgaagt ccggcctgcc ggttatgcgc
    11881 ccgctgttcc tgcattacga agacgacgcg cagacctatt ccctgaaata tcagtacctg
    11941 ttaggtcgcg acattctggt tgctccggta cacgaagaag gtcgcagcga ctggacgctc
    12001 tatctgccag aggataactg ggtccacgcc tggaccggag aaaccttcca cggtggagaa
    12061 ataactgttg aagcgcccat cggcaagccg ccggtctttt atcgcgccga cagcgaatgg
    12121 gctgcactgt tcgcctcgtt aaaaaacatc taatcatgcc cgcccgtggg gaaactcacg
    12181 ggcaaatgga gaataacgat gagtcacatc acaacggaag atccggcaac tttgcgcctg
    12241 ccctttaaag agaaactctc ttacggtatc ggcgatctgg cctctaacat cctgctggat
    12301 attggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc tggcacctat
    12361 ggcggcatta tctttttgat ctcgaaattc tttaccgcct ttaccgatat gggaaccggc
    12421 atcatgttgg attcccgacg caagatcggt ccaaaaggta aattccgtcc ttttattctg
    12481 tatgcgtcat tcccggttac cttattggca atcgctaact ttatcggcac accgttcgat
    12541 gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact gtttttcagc
    12601 atgatgaact gctcctacgg cgcaatggtt cccgccatta ccaaaaaccc caacgaacgc
    12661 gcctcgctgg cagcatggcg tcagggcggc gctacgctgg gcctgctgct gtgcacggtg
    12721 ggattcgtgc cggttatgaa tcttatcgag ggtaatcagc aacttggcta tatcttcgcc
    12781 gccacgctgt tttcactgtt tggcctgctg tttatgtgga tctgctattc gggcgtgaaa
    12841 gagcgttatg tcgaaaccca acctaccaat ccggcgcaaa agccaggcct gttgcaatct
    12901 ttccgcgcaa ttgccggtaa ccgcccactg ttcattttgt gcattgccaa cctctgtacc
    12961 ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta cgtgcttaac
    13021 gatcccatcc tgttgtcata tatgggattt ttcagcatgg gctgtatttt catcggcgtg
    13081 ttcctgatgc ctggtgcagt caggcgtttt ggtaagaaga aggtctatat tggcggtctg
    13141 ctgatctggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc ggtcaacttt
    13201 gtggctttct cctgcctggc gttctttggt tcggcgtttg ttaacagcct gaactgggcg
    13261 ctggtttctg acaccgtcga gtacggcgag tggcgtaccg gcgttcgttc ggaaggaacg
    13321 gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc
    13381 ggctggatgc tgacgcaaat cggctatgtg ccgaacgtgg cgcaggctga ccacaccatt
    13441 gaagggttgc gccagctgat cttcatctac ccaagcgcac tggcggtagt caccattgtg
    13501 gcgatgggtt gcttctacag tctgaacgag aagatgtacg tccgcattgt tgaagaaata
    13561 gaagcccgta aacgcacggc gtaataataa acgacgccct gcggggcgtt ataaggagtg
    13621 attatgtctg accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat
    13681 ggtatgggcg acgttggttc gaatttaatg cttagcatcg gtactctgta tctcctcaaa
    13741 ttttataccg atgaattagg gatgccagct tactatggcg ggattatctt tctggtggcg
    13801 aagtttttta ccgcgtttac cgatatgctc accggatttt tactcgattc gcggaaaaat
    13861 attgggccta aaggcaaatt tcgtcccttt attttatatg cagcggttcc ggcggcgtta
    13921 attgcaacgc tccagtttat cgccaccacc tttagtttgc cggttaaaac gacgattgcc
    13981 accgcgctgt ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg
    14041 atgatcccgg caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag
    14101 ggtggtgcaa ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt
    14161 ttttctgact caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt
    14221 atttttatga tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcaccaacc
    14281 ggacataaag ccagtattct caaatctttt tgcgcgatat ttcggaatcc gccattgctg
    14341 gttttatgca ttgctaactt gtgtaccctg gcggcattta atatcaaact ggcgattcag
    14401 gtctattaca cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc
    14461 agtatgggat gcatcctcat cggcgtatta ctggtgccat taactgtaaa atgttttggt
    14521 aaaaaacagg tttatttagc tggcatggtg ctatgggcag tgggtgatat cctgaattat
    14581 ttctggggaa gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg
    14641 gcctttgtaa acagcctgaa ctgggcgctg gtaccagata ccgtagatta cggtgaatgg
    14701 aaaaccggta ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt
    14761 tctgccgcac ttgctggctt cttgccaggc attatgctga cgcaaatcgg ttatgttccc
    14821 aacatcgcgc aaagtgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct
    14881 tgtgcactgg caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa
    14941 cggtttgcgt tgattattga ggaaatcaac caacgcaaaa ataaagaaat tgagacagaa
    15001 gaaaaaacgg cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt
    15061 ggcggcctcc ctacgtttaa aaatggactt attatgaaaa atattaatgc aataatttta
    15121 ttatcttctc tgacctctgc ctcagtattc gctggcgcat acgtcgaaaa cagggaggca
    15181 tataatctcg cctccgatca gggcgaggtt atgctacgtg tgggttataa cttcgatatg
    15241 ggcgcgggta ttatgttaac caatacctac acctttcagc gagaagatga actaaaacat
    15301 ggatataacg aaatcgaagg ttggtatccg ttatttaaac caaccgataa attaaccatc
    15361 cagcccggtg gcttaattaa tgataagagt atcggttcgg gtggtgcagt gtatctggac
    15421 gtcaactata aatttgtacc atggtttaat ctgacagtac gaaatcgcta taaccataat
    15481 aactatagtt caacagattt gagcggggaa ctggataata atgacaccta tgaaattggt
    15541 acctactgga attttaaaat caccgataag ttctcctata catttgagcc acattatttt
    15601 atacgcgtaa atgactttaa tagcagcaat ggaaaagatc atcactggga aatcactaac
    15661 accttccgtt atcgcattaa tgaacactgg ctgccttatt tcgaattacg ctggttagat
    15721 cgtaatgtcg aaccgtacca ccgcgagcaa aaccagattc gtatcgggac gaagtatttc
    15781 ttctgatgtg aaactgccgg atgcggcgta aacgccttat ccggcctacg gttgatacgg
    15841 ttttcgttgg ccggattaag cacatccggc cattaattaa gcttcagccg ccagcatctc
    15901 tttcttacgt tggctaatac gcttaaccag cattacggaa accaccgcac cacagaatgc
    15961 gaagcaggcc ataatgccaa acacgatttt gtagccaata atccccggat ttaaatccag
    16021 aatgtagcca tacagactga agcagaacat cgccggggcg taaccaataa agctacccaa
    16081 cgccatcgcc gcgcctgttt tattttcagc aatttttgct tcgccgatag gtgcaaaaaa
    16141 tacggctcgc tgtgtaaaga ctatcgcgcc aaagcccagc gtacatgcca tccctaaata
    16201 gaccggcata ctttcgtgag gtagcataat caacagtacg agcgcagcgg tactgatgat
    16261 aaaggtgtag catagatatt tactcggcga tttcaggatc ttatctgaaa tcatgccacc
    16321 aatcggtcca ccaatcattt tcagacagta ttggttaatg atgccgtaag cccccaccag
    16381 cgcaacgggc aatagataga tgtttttcag gaatggaatg aagaatgtca ggccacagta
    16441 aaccgcgtaa acgaagaaga cgttgaaagc gataagccag atggttttgt ctctcagcac
    16501 cgaggtcata ctggtgtttt gcgatgctcc gtcttctttt ttcacctcaa cggacggtgc
    16561 ctcttctttg tcattcagga caaagaaaat aataatgcct acggcaatca caataaggga
    16621 atagaaccag atgcctgctt taaaacccaa taagccactg ccaaaccagg taaatactgc
    16681 caacgcagaa aatgccacca cggtatcgac aatgccacgc cctgtttcga agaagccaaa
    16741 caaccgacct tgttgttcgc tattgcccaa tcgacttacc gacttgagca agaccggcca
    16801 gttcatcatg tcgcaagtaa cgccaaatag cgcccagaca aagaggatgc cccaataccc
    16861 cggcattgtc gtcaaataaa cacccagtaa tcctgtcgca atgagtgaga atgacatggt
    16921 gtatctgcgc ggtagtttat cggcaaaata aatagacaga aaaaagccca ctgtggtgac
    16981 aaatgagttt accgacatgg cattaccaat ttgaccattg gtcaaatgga aatattcctg
    17041 catcgggata taaaacgcat ctttcagcga cggtaattta taaattgtac cgccacacag
    17101 tgttaataga ctaaataacg cccatttctt tttcgtgagc ataacatccc ctaagcgatt
    17161 tttaaaatca ctgttttaaa ggcaataaga taccgaccat tcatatggaa tggttttgtt
    17221 aactgcgtat gagttaaaac ttattagtgg ttcagaagat ggtttgcctc ttgtttgatt
    17281 tgttgtagta cgttacgcac atgatttatc tgattaattg catcttcttt ttctttacgt
    17341 aaccgcgcat ccagtaaatg attttctggt aggggtgttt cactcatctg gcgataaggg
    17401 atccacggat tatcatcttc gtcttcaaag cggaacgccg gcgcatagta atcaacctct
    17461 tcttccaggc catatgccgt cacctgcggc tcatcatcac caaggcagat aaggtgcgtt
    17521 aataacgctt tgaacggcat atcaccctga cctgaaatac aggctttatg acccaggcca
    17581 ccctgttctt taacgatcaa ggcatcttta atatggacct gggtaatatg cggtgccatc
    17641 gtttttaagg cgtcgatggg atgctcattt gcattgatca tgttcgcaaa atcaaacagt
    17701 aaggaaagag actccatctc actttctttg accagcgaca ccagctcatg actttttaaa
    17761 tcttcatgct gctcaatcgt aaaagtcagg ccgcagtcct gatacgtttc ccgtacatag
    17821 gcaatgtcgt tagcgataat cgataatacg tcgcgcaaat taccttcata acgtggataa
    17881 aaacgtacgg acgatgcccc agttttcaac gcgatggcga cggcttcgtc aatagatgcc
    17941 ttatctgagg cgctggtttc aatatgaata tcaaggttca gacggcgggc tttatctcca
    18001 aaggcagaga gttctttatc gtccatatta ccaagagaaa aacgctcgcc atccagaaca
    18061 tggattttga cgccacgtag attattttca cttgcgatat caagtaaatc agcaggcaac
    18121 actctttcca gccgcatatt taaatgaaag gcataagcat gtaaataaag aggaagagtg
    18181 tcgacacgtt gtagaatctt tcttgcgtta tttattgtca tcataaaaca cctacagatt
    18241 aatgatgagg tctacggcct caaatcgcca atattcaata tctatctgga caatattgcc
    18301 ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc atacccgcag aaccgccaag
    18361 atacttgctg atatcgcccg gcattctgac gggtttgaaa atcaattttt ttactaccgt
    18421 ctctttttgg tagcactttt cccagactgc agaaaatgag tggttttcaa gttgttcaat
    18481 aaaacccgga gcaacttcag gattaatata tgtttcgtga tagaaaactt tatgtccttc
    18541 cagcgccccc cagccggtga tgcgatagag ttcagttgat ggtgtcactg caatcagtgg
    18601 cgcgagcgtc tcgggaatat cgctggtacg gtttttctcg gtaaaccccc aggaaggctc
    18661 tcgcccttgc tcaattgcgg cccgctgaaa gctcgccgac agctccggac tgtaattaaa
    18721 tcgcggctgg gtcacgaacc accccttacg atctttacgg aagattttgg attctgcctc
    18781 gaggttcaac aacgcctggc gcagcgtcat acgtttaatg cccagcagtt ctcccagctc
    18841 tctttctgaa gggagttttc cacccggcgt ggtgatgcca tctttcaacc accgatccag
    18901 tttctctttt gcattttcta cggtggattg attctccgcc attgtttaat ccttttggtt
    18961 taaaccagat gaagcatctt acgcttacgc caaaaaagaa agcaattaac ggcgtatgac
    19021 tttgcagata attgtgacaa agctctctat tgacgtaatc acagacttat aagggagata
    19081 ctcgcattgg tgtgcgctca cgacaggtaa aaaaaacctg ccagcgatgg caggtttttt
    19141 ataactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcggcg acgatcgttt
    19201 tccgtcaggt gacgtttacg aatacggata gaggtcggag tcacttctac cagttcgtca
    19261 tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac cagaacaacg
    19321 gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag gcagtttaca
    19381 gtcaggtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac ttctgcaccg
    19441 tgaccgagga acagcttacc gcgatcctgc agaccgaaca gcgcgaacgc gaccgcttta
    19501 ccctgaccgt tagagatcag tacaccgttc tgacgctgac ccacttcgcc cggacgtacg
    19561 tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat ggtcatgaac
    19621 tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg tacgcggcct
    19681 ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc ctgcattaca
    19741 gaaccctgat gctgttcttc aacgtccaga gtcacgtttt catacggctc ttgtttacga
    19801 ccgtcgattt cacggaagat aactttcgga cgggataccg ccagttcgaa accttcacga
    19861 cgcatgtttt cgatcagaac agacaggtgc agttcgccac gaccagaaac gcggaacgca
    19921 tcagcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc tttgttcaga
    19981 cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa cggcgaggtg
    20041 ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg cagcgcttca
    20101 acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgccaagacc cgtgatcgcc
    20161 acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag gtggcccagc
    20221 actttaccga ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt aacctgctgg
    20281 ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacaccaac atagctgttg
    20341 taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc cggcgcagga
    20401 acgtggtcaa caatcgcctg gtacagcgga gtcatgtctt ccgccatatc ttcgtggtcc
    20461 agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag ctgctcgtcg
    20521 gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc aggacgcgcg
    20581 ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc aaacgctttt
    20641 ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac cagcagcact
    20701 gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg ccccggggta
    20761 tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc gaggatggta
    20821 atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt ttcggcacga
    20881 gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt accatggtct
    20941 acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc tcaggcatta
    21001 gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca caaacatcct
    21061 ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata aaaagggtta
    21121 tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact atattggtgc
    21181 aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac gccttttagg
    21241 ggcaatttaa aagttggcac agttttcgct ttatcttttt tacggcgaca cggccaaaat
    21301 aattgcagat ttcgttacca cgacgacaat gaccaatcca ggagagttaa agtatgtccg
    21361 ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat ttgcgcttca
    21421 ccgataccaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg aatgctgaat
    21481 tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa ggcattaacg
    21541 aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg ttcttcgccg
    21601 attctaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa ggctatgacc
    21661 gtgacccgcg ctccatcgcg aagcgcgctg aagattacct gcgttccact ggcattgccg
    21721 acaccgtact gttcggacca gaacctgaat ttttcctgtt cgatgacatc cgtttcggat
    21781 catctatctc cggttcccac gttgctatcg acgatatcga aggcgcatgg aactcctcca
    21841 cccaatatga aggtggtaac aaaggtcacc gtccagcagt gaaaggcggt tacttcccgg
    21901 ttccgccggt agactcggct caggatattc gttctgaaat gtgtctggtg atggaacaga
    21961 tgggtctggt ggttgaagcc catcaccacg aagtagcgac tgctggtcag aacgaagtgg
    22021 ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac aaatatgttg
    22081 tgcacaacgt agcgcaccgc ttcggtaaaa ccgcgacctt tatgccaaaa ccgatgttcg
    22141 gtgataacgg ctccggtatg cactgccaca tgtcgctgtc taaaaacggc gttaacctgt
    22201 tcgcgggcga caaatacgca ggtctgtctg agcaggcgct gtactacatt ggcggcgtaa
    22261 tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct tataagcgtc
    22321 tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcacgtaac cgttctgctt
    22381 ctatccgtat tccagtagtt tcttctccga aagcgcgtcg tatcgaagta cgtttcccgg
    22441 acccggcggc taacccgtac ctgtgctttg ctgccctgct gatggccggt cttgatggta
    22501 tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac ctgccgccag
    22561 aagaagcgaa agagatccca caagttgcag gctctctgga agaagcactg aacgagctgg
    22621 atctggaccg cgagttcctg aaagccggtg gcgtgttcac ggatgaagcg atcgatgcgt
    22681 acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat ccggtagagt
    22741 ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa caacgccggg
    22801 atttagttgc cgtggaaact ttcagcccat ctctgcatgg gcttttttct ccgtcaattc
    22861 tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaatg gtgcaacctg
    22921 ttcaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga tcctcaactc
    22981 gctgattaac agtattttgt taatcgatga caacctggcg atccattacg ccaaccccgc
    23041 cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacaccgt taccggaact
    23101 gttgagctac ttctcattaa atatcgagct gatgcaagaa agtctggagg cggggcaagg
    23161 ttttaccgat aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc tttctgtgac
    23221 ggcccagcgt atgccggacg gcatgatcct gctggagatg gcgccgatgg ataaccagcg
    23281 ccgcttaagc caggaacagc tacaacatgc tcaacaagtt gctgcccgtg atttagtgcg
    23341 cggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg cgcagctact
    23401 cagcaaagca ttacctgacc cgtcactact ggaatatacc aaagtgatta tcgaacaggc
    23461 ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg gtacgcgcgt
    23521 gaccgaaagt attcacaaag tagctgaacg cgtggtaacg ctggtgtcga tggaactgcc
    23581 ggacaacgtg cggttgattc gtgattacga tcccagcctg ccggaactgg cgcacgaccc
    23641 ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg cgctggggcc
    23701 ggagggtggt gaaatcattc tgcgtacccg caccgcgttt caactgacct tacacggcga
    23761 gcgctaccgg ctggcggcgc ggattgatgt ggaagataac gggccaggca ttccgcctca
    23821 tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca ccgggcttgg
    23881 cttatccatc gctcgtaatt tgattgatca gcattcaggc aaaattgaat ttaccagttg
    23941 gccagggcat accgagttct cggtttacct gcctatcagg aaataaaggt gacgtttatg
    24001 caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt gcttgaacgt
    24061 gcgctcgctg gagcgggttt aacctgtacg acatttgaga acggcgcgga ggtactggag
    24121 gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc gggaatggac
    24181 gggctggcgc tgctcaagca gattaaacag cgccatccga tgcttccggt catcattatg
    24241 accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc gttcgattat
    24301 ctgcccaaac cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc catcagtcat
    24361 taccaggaac agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac cgatatcatc
    24421 ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg gtcggctttc gcgttcttct
    24481 attagcgtgc tgattaacgg cgaatccggt accggtaaag aactggtcgc tcatgccctg
    24541 catcgccaca gtccgcgagc caaagcgccg tttatcgcgc tgaatatggc agctatccca
    24601 aaagatttga tcgaatccga actgtttggt cacgagaaag gcgcatttac cggcgcgaat
    24661 accattcgtc aggggcgttt tgaacaggct gatggcggta cattatttct cgatgaaatt
    24721 ggcgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga cggtcagttt
    24781 taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc ggattatcgc tgccactcac
    24841 cagaatctcg aacagcgggt gcaggaaggt aagttccgtg aggatctgtt ccaccgcctg
    24901 aacgttatcc gcgttcatct gccgccactg cgtgagcgtc gggaagatat tccccgcctg
    24961 gcacgccatt ttttacaggt tgctgcgcgc gaactgggcg tagaagcgaa gttgctgcat
    25021 ccggaaaccg aagctgctct gacgcgtctg gcgtggccgg gtaacgtacg ccagctggaa
    25081 aacacctgtc gctggctaac agtgatggcc gccgggcagg aagtgttgat tcaggatttg
    25141 cccggtgaac tgtttgaatc aacggttgcg gagagtactt cgcaaatgca accggacagt
    25201 tgggcaacgc tgttagcaca gtgggcagat cgggcgctac gttccggtca tcaaaatctg
    25261 ctttccgaag cgcagccaga gctggagcgg acgttactga cgaccgcgtt gcgacatacg
    25321 caggggcata aacaggaagc ggcgcggcta ctcggctggg gccgcaacac tctgacgcgt
    25381 aagttaaaag agttagggat ggagtgattc acggcttgtg tgtaaagatt gattattgag
    25441 cgcaaattgc tggtattttg cgctttactg ttccgataag ttcagtatga tcttgcccgg
    25501 aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag tggcgcagtt
    25561 gacagccaca caccgcaaac tgccgttgct gccgtgctgt gtgccgcgat gattgggctg
    25621 tttagttgag tttcagattg ctgatgaagt gctctttgtt catgcggatg cggcgtgaac
    25681 gccttatccg gcctacaaaa gcaatatttt caacaggttt tgtaggcctg ataagcgtag
    25741 cgcatcaggc aatcttgttc tcagcacatt gatggcgctt tcgttttcta ctttgtaaac
    25801 gaagcgccat tcactattta aatcacccgt gagaactgct gcatccgcgc tttctggcgc
    25861 agataggtat caaagcacat gcaaatgttg cggatcagca agcgaccttt cgccgtcacc
    25921 tgtattccct tctcatccac atccaccagc ccatcttttg ctaacggggc gagcagcttg
    25981 agatcttccg caaagtaatc agcgaagtgc aaatcccact gtttctcaat aggggcgtaa
    26041 tccagacgga agttgcagat gagcgactta atcacatcgc ggcgaataca gtcatcacgc
    26101 gttagcgcaa taccacgcca cagcgcattg ccttgttcat ccacttgctg atagtactgc
    26161 ttcaactctt tctggttctg cgcgtagcag tcgccaatca tgctgatggc ggaaacgccc
    26221 atccccagca gatcggtatc gccctgagtg gtgtagccct ggaaattacg atgcagcacg
    26281 ccttcacgct gggccaccgc cagctcgtca tccggacggg caaagtgatc cataccgata
    26341 aactgatagc ccgattgcgt caggaaggcg atggtttcct gcaggatatc gagtttttgc
    26401 tgcggactcg gcaggtcagc atctttgatt ttgcgctgag cagcaaaaat ggtcggcaga
    26461 tgcgcatagt taaagacgct cagacggtca gggtttagct ccgccacgcg tttcagggta
    26521 aaggcgaaac tttccggcgt ctgtttcggc aggccgtaaa tcaggtcgat gttggtggag
    26581 gtaaagccaa tctcacgcgc atggttaagc agcgcaaaga taaactcttc atcctgctcg
    26641 cggttaacca gacgttgcac ttctttgttg aagtcctgca cgcccatgct caggcgatta
    26701 aagccttcgg cgcgtaaatg atcgagtaca tccagttcga tttcccgcgg atcgacttcg
    26761 atcgaaatct ccgcatcggc attgaactgg aagttttcgt gcagcagctt catcaggcgg
    26821 ctgatttgcg ctttattcag atacgtcggc gttccaccgc cccagtgcag ctggctgacg
    26881 tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt gctccagcgc gtccagatac
    26941 tgatcggcct tgtgctgctg gcgagtaaca atcttattgc agccgcagaa gtaacaaagc
    27001 ttatggcaga acgggatatg tacgtagaga gataatggac gctcaggata gcgcgccacg
    27061 gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact ccagcgcggt cgggtacgag
    27121 gtgtatcgtg gcccggaata gttatatttc tggatcaggg ccagatccca gtcgatttgc
    27181 tgtacagaca tgctcactcc ttccgatggc gtctctggcg gctacggcgt accggcggat
    27241 gcccgtttcg ggtcatcagc cggttgcgca gccacttttg tcgccgcgac aaccgctgta
    27301 gtttaacgaa taaccacacc agataacata taaccggaag ggttatcagc agaacgggaa
    27361 ggcccacggc ggcaatccgt tagttgcccc gcagcagacg catcatgtct tcttgcttct
    27421 cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc gagtttctgc atcaactcat
    27481 caatacgatc cagtttagca tccacccagg attgctcttc ggcactcagg gtttcgcctg
    27541 cttccagacg ttccagcagc gcatccagac gctcatccgt ttccagtaat tccaactccg
    27601 cctgcggtga aagcataggt ttctcactct tcggtttgtg ctgtttggtg actttttcag
    27661 tcacgcccaa tggaataggg gttttactgc caatacgtgg atcttttggt gcgttctggc
    27721 ctttgctgcc tgacgtggtg ttaccgcccg ctgcgcggct gcccggcgca tgaccacgac
    27781 gttttttctg acgcttgcgg tcacgagctt cctgattcag ctcttcgcgc gttttgcgac
    27841 gcgcttttgc atgacctttg ctgcgtgatg gtgaagatgg tttcataatg gttcgtcttt
    27901 tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa
    27961 aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa
    28021 tccttgcagg ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc
    28081 catttccgtt taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct
    28141 cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg
    28201 tgctacttta cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt
    28261 cagagtttta tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga
    28321 attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt
    28381 acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca
    28441 aagctttgac tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt
    28501 gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt
    28561 gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa tcagaaaagc
    28621 ctggctcgta cctcaaaaac ccctggacgc acccagctta tcaacctgtt tgaagtggct
    28681 gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg
    28741 aagcgcaaat ggcagcgcgc gctcggcgag tacctcgaaa aacgtcagag cctgcaaggt
    28801 ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag
    28861 tgggcggtag acagcaatat cgccgtgctg gtactgctga ccaaagcgga caaactggca
    28921 agcggcgcac gtaaagcaca attgaatatg gtgcgtgaag cggtgctggc gtttaacggt
    28981 gatgtgcagg ttgaaacgtt ttcttcgctg aagaaacaag gcgtggacaa gctgcggcag
    29041 aaactggata cctggtttaa cgagatgcag cctgtagaag aaacgcagga cggcgaataa
    29101 ttttcttacc ttaatgctta tgccggatgt ggcgtatccg gcccataaat tcaatagatt
    29161 acaattctac cgttggcctg ataaacatgg tgcatcaggc attacggatc tttcctttcg
    29221 cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc
    29281 gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact
    29341 aactttactc ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg
    29401 cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa
    29461 aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc
    29521 caccagcaac ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc
    29581 gacggcatcg acatcatctt tatggacttc aaataccagt tcatcgtgta cctgcatgat
    29641 catacgtaca cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt
    29701 gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc
    29761 acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt
    29821 ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc
    29881 agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa
    29941 ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc
    30001 gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt
    30061 tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttgt cacgcgagag
    30121 atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc
    30181 tggcgcaata aacgcctggc ggatacggcg accttcttcg ttacgtaccg gaatgttttg
    30241 caggttagga tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt
    30301 atgtacacgc ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aggtcgattt
    30361 cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag
    30421 ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt
    30481 aatgccctgt ttttcaaaca gaatggtttg taactgcttg gtggaagaaa ggttaaattc
    30541 ctcacctgca atttcatgcg ctttcttttc cagctcagcc agacgcaagg taagctcttc
    30601 agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag
    30661 caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg
    30721 cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta
    30781 acgtccggcc tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc
    30841 aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tctgcgaggc tgtccatatc
    30901 gtgacggcca gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc
    30961 acgcagctca atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac
    31021 cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga
    31081 gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg
    31141 ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc
    31201 ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc
    31261 ttcatcaagg atggtgacgt agttgtcata agaaatcacc gttgccgtca cttctggtgc
    31321 ttcgtctgca acactggttt cctgtggctt cgcggcgggt tttgcccctt tggcctgtaa
    31381 ccatttgccc gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc
    31441 caacaactct tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc
    31501 aacgtcggtt ttaatcgtcg ccagctggta tgagagataa gcaacttctt tgttttgctc
    31561 gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc
    31621 ggcatacagc gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc
    31681 gacgcccggt acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaaatcgat
    31741 gatcagttct ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt
    31801 cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc
    31861 agtgctgatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac
    31921 gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc
    31981 gtgcaagggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta
    32041 atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg
    32101 cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc
    32161 ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata
    32221 agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc
    32281 tgtttatcag attatgggta aaggatgcca cagacaggat aaaaacatga gtttttcacc
    32341 gcttttcgtg aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc atgtggataa
    32401 ctttgtgcat aaattattta c
//
LOCUS       DAPGRN010000049        32362 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPGRN010000049 DAPGRN010000000
VERSION     DAPGRN010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.049"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..1235
                     /gene="yiaY"
                     /locus_tag="RHW33_002405"
     CDS             84..1235
                     /gene="yiaY"
                     /locus_tag="RHW33_002405"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV1645901.1"
                     /translation="MAASTFFIPSVNVIGADSLADAMNMMAEYGFRRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSIIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1365..2660
                     /locus_tag="RHW33_002406"
     CDS             1365..2660
                     /locus_tag="RHW33_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDV1645902.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVEVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLIRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            2768..4306
                     /gene="aldB"
                     /locus_tag="RHW33_002407"
     CDS             2768..4306
                     /gene="aldB"
                     /locus_tag="RHW33_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV1645903.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKNRYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(4525..4680)
                     /locus_tag="RHW33_002408"
     CDS             complement(4525..4680)
                     /locus_tag="RHW33_002408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645904.1"
                     /translation="MAKFMIKNRKTSTMQIKRIKPSIIYRLLLIVLGIPMVIYGLVCP
                     LTIETRD"
     gene            4851..5174
                     /locus_tag="RHW33_002409"
     CDS             4851..5174
                     /locus_tag="RHW33_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV1645905.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            5180..6316
                     /locus_tag="RHW33_002410"
     CDS             5180..6316
                     /locus_tag="RHW33_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1645906.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMVANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELNEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(6313..7287)
                     /locus_tag="RHW33_002411"
     CDS             complement(6313..7287)
                     /locus_tag="RHW33_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1645907.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            7411..8151
                     /locus_tag="RHW33_002412"
     CDS             7411..8151
                     /locus_tag="RHW33_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV1645908.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDTFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(8281..10251)
                     /locus_tag="RHW33_002413"
     CDS             complement(8281..10251)
                     /locus_tag="RHW33_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HDV1645909.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKVPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDSVFGPDESKLHGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPIAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEEKHCHP"
     gene            complement(10262..11662)
                     /locus_tag="RHW33_002414"
     CDS             complement(10262..11662)
                     /locus_tag="RHW33_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1645910.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            11888..12703
                     /locus_tag="RHW33_002415"
     CDS             11888..12703
                     /locus_tag="RHW33_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1645911.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDNDPRWLTHLS
                     VPQQTKVVDPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            12705..12845
                     /locus_tag="RHW33_002416"
                     /pseudo
     CDS             12705..12845
                     /locus_tag="RHW33_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
     gene            12947..13420
                     /locus_tag="RHW33_002417"
     CDS             12947..13420
                     /locus_tag="RHW33_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV1645912.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13571..14824)
                     /gene="avtA"
                     /locus_tag="RHW33_002418"
     CDS             complement(13571..14824)
                     /gene="avtA"
                     /locus_tag="RHW33_002418"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV1645913.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLHEKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRMSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15002..17032)
                     /gene="malS"
                     /locus_tag="RHW33_002419"
     CDS             complement(15002..17032)
                     /gene="malS"
                     /locus_tag="RHW33_002419"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV1645914.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADVIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLTVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVVWAGQQ"
     gene            17352..18176
                     /locus_tag="RHW33_002420"
     CDS             17352..18176
                     /locus_tag="RHW33_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV1645915.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            complement(18284..19462)
                     /gene="xylR"
                     /locus_tag="RHW33_002421"
     CDS             complement(18284..19462)
                     /gene="xylR"
                     /locus_tag="RHW33_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV1645916.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(19540..20721)
                     /gene="xylH"
                     /locus_tag="RHW33_002422"
     CDS             complement(19540..20721)
                     /gene="xylH"
                     /locus_tag="RHW33_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV1645917.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            complement(20699..22240)
                     /gene="xylG"
                     /locus_tag="RHW33_002423"
     CDS             complement(20699..22240)
                     /gene="xylG"
                     /locus_tag="RHW33_002423"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV1645918.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRNAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            complement(22318..23310)
                     /gene="xylF"
                     /locus_tag="RHW33_002424"
     CDS             complement(22318..23310)
                     /gene="xylF"
                     /locus_tag="RHW33_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1645919.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            23676..24998
                     /gene="xylA"
                     /locus_tag="RHW33_002425"
     CDS             23676..24998
                     /gene="xylA"
                     /locus_tag="RHW33_002425"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV1645920.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            25070..26524
                     /gene="xylB"
                     /locus_tag="RHW33_002426"
     CDS             25070..26524
                     /gene="xylB"
                     /locus_tag="RHW33_002426"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV1645921.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECALLEARVPLSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            26693..27034
                     /locus_tag="RHW33_002427"
     CDS             26693..27034
                     /locus_tag="RHW33_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1645922.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            27080..27517
                     /locus_tag="RHW33_002428"
     CDS             27080..27517
                     /locus_tag="RHW33_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV1645923.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(27559..28554)
                     /gene="wecH"
                     /locus_tag="RHW33_002429"
     CDS             complement(27559..28554)
                     /gene="wecH"
                     /locus_tag="RHW33_002429"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV1645924.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRALGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            28729..29028
                     /locus_tag="RHW33_002430"
     CDS             28729..29028
                     /locus_tag="RHW33_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HDV1645925.1"
                     /translation="MMMNSFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            29123..30034
                     /gene="glyQ"
                     /locus_tag="RHW33_002431"
     CDS             29123..30034
                     /gene="glyQ"
                     /locus_tag="RHW33_002431"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV1645926.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            30044..32113
                     /gene="glyS"
                     /locus_tag="RHW33_002432"
     CDS             30044..32113
                     /gene="glyS"
                     /locus_tag="RHW33_002432"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV1645927.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT         8055 a         7916 c         8336 g         8055 t
ORIGIN      
        1 gctaaacaat aatactgatc atattttcga atgaaaatcc tcagtaagct gcccgccctt
       61 ttttacactt tcaggagtgt gttatggcag cttcaacctt ttttattcct tctgtgaatg
      121 tcatcggcgc tgattcattg gctgatgcaa tgaatatgat ggcagaatat gggtttcgcc
      181 gtacattgat tgtcactgac agtatgttaa cgaaattagg tatggcgggc gatgtgcaaa
      241 aagcactgga agaacgcaat atttttagca ttatttatga tggcacccaa cctaacccca
      301 ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga aaataattgc gatagcgtga
      361 tctccttagg cggtggttct ccgcacgact gcgcaaaagg tattgcgctg gtggcagcca
      421 atggcggcga tattcgcgat tacgaaggcg ttgaccgctc tgcaaaaccg cagctgccga
      481 tgatcgccat caataccacg gcgggtacgg cctctgaaat gacccgtttc tgcatcatca
      541 ctgacgaagc gcgtcatatc aaaatggcga ttgttgataa acatgtcact ccgctgcttt
      601 ctgtcaatga ctcctctctg atgattggta tgccgaagtc actgaccgcc gcaacgggta
      661 tggatgcctt aacgcacgct atcgaagcgt atgtttctat tgccgccacg ccgatcactg
      721 acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa cctgccgtta gccgttgaag
      781 atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc ccagttcctt gccggtatgg
      841 cgttcaataa tgcttctctg ggttatgttc atgcgatggc gcaccagctg ggcggtttct
      901 acaacctgcc acacggtgta tgtaacgccg ttctgctgcc gcatgttcag gtattcaaca
      961 gcaaagtcgc cgctgcacgt ctgcgtgact gtgccgctgc aatgggcgtg aacgtgacag
     1021 gtaaaaacga cgcggaaggt gctgaagcct gcattaacgc catccgtgaa ctggcgaaga
     1081 aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa agaagaagat ttcgcggttc
     1141 tggcgactaa tgccctgaaa gatgcctgtg gctttactaa cccgatccag gcaactcacg
     1201 aagaaattgt ggcgatttat cgcgcagcga tgtaatcatc atttccacaa cggctggcaa
     1261 attgtcagcc gctttttcaa ctatctcaaa acttgctttt tttgagatag tttctctttc
     1321 ctgctataca ctatctcaat attgcgcttt tttgagataa gcacatggct atcaaatcac
     1381 ctcccggact tattccttta tcacacttat cgggtgagga actcctggcc cacctgcgct
     1441 ttaaccgggt taccgatgaa aaaggacgtt atctgccttt tgatgagttg cagtaccgta
     1501 tcaaaaaagg agaaaacgtc gaagtggcgt ggacgcttac ccgtctggcg cgcaacgcgg
     1561 cgatccaacg aattaattat tgcaatgaag cgggtgaaca ggccggattc aacattaccc
     1621 cggtgattgc agaagcgtgc gagcttgtcg acaaacgtgc taccgcgctg gcgctgaaag
     1681 atcagactga acggttacgc ggggccggag ccgagttaag ccagttacgc ctggaagagc
     1741 cgatcaccag ctcccagctg gaaggtgcga atactacaac gctggtggcg agaaaaatgc
     1801 tggaaacagg acggtcgcca cgcactgaag acgagcatat gattgcggga aatgcccggc
     1861 tgatggcgga aattccgcac ttactcgcag aaccgctgac acctgcgctt attcgccagt
     1921 tacatgccat tggtatgggt ggaatcaatg acgcgaaata tcgtccggga gaatttcgtg
     1981 aaacagatga tgttgtgatc gccgactatg atggcaatat agtgcatcaa ccgcccgctg
     2041 cggcattgtt accggaacgg ctggaaaaag tttgccagtg gttaaacagc catgaagggt
     2101 atattcaccc gttaatccgt gcctgcattc tgcattttat gctggcccat gaacatccat
     2161 ttcgcgatgg caacgggcgc accagtcggg cgctgtttta ctggtatatg ctgaaatcgg
     2221 gctacgatgt ctttaaatat atttccatca gccggttgct tcatgctgca ccggtcaaat
     2281 atgccgccag ctaccagtac actgaatcag atggtatgga tctgacttat ttccttgaat
     2341 atcaggcggg tgtcattaaa cgtgctctgc aaaactggca gcagcatatc gatgaaatta
     2401 cacaacgaag cgcaaaactc gatagtgtgc tgtttagctc tggtgtgctt aaacgcctga
     2461 atccgcgcca ggtaacgctc ctgaatgtga tgctcgccaa tccggggaaa gagtacacag
     2521 ttgctgaaat tagcgcgagc ctgggcgtct ctgataatac ggctcgagct gatttacgga
     2581 caattgttaa agaagggttt gcgcaagaga agaaaattaa tgaccagcaa gccgtgtact
     2641 tcgcgcatta tccgctgtaa cccttgcccg taaattcgtg atagccgtcg taaagctgtt
     2701 accgactggc gaagatttcg ccagccacgt ctacccttgt tatacctcac accgcaagga
     2761 gacgatcatg accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc
     2821 cctcaagtta aaaaaccgct atgacaactt tattggcggc gaatgggtag cccctgccga
     2881 tggtgagtat taccagaacc tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc
     2941 ttcgggcaaa cgagacatcg atctggcgct ggatgctgcg cacaaagtga aagataaatg
     3001 ggcgcacacc tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga
     3061 acaaaacctc gagctgttag cgacagctga aacctgggat aacggcaaac ccattcgcga
     3121 aaccagtgct gcggatgtac cgctggcgat tgaccatttc cgctatttcg cctcgtgtat
     3181 tcgggcgcag gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca
     3241 tgaaccgtta ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag
     3301 ctggaaaatg gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct
     3361 taccccgctt tctgtactgc tgctaatgga aattgtcggt gatttactgc cgccgggcgt
     3421 ggtgaacgtg gtcaatggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg
     3481 catcgccaaa gtggcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc
     3541 aacgcaaaac attattccgg tgacgctgga gctgggcggt aagtcgccaa atatcttctt
     3601 tgctgatgtg atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact
     3661 gtttgccttt aaccagggcg aagtttgcac ctgtccgagt cgtgccttag tgcaggagtc
     3721 aatctacgaa cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa
     3781 cccactcgac agcgtaacgc aaatgggggc gcaggtttct cacgggcaac tggaaaccat
     3841 cctcaactac attgatatcg gtaaaaaaga gggcgctgat gtgctcaccg gcgggcggcg
     3901 taagctgctg gaaggcgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg
     3961 tcagaacaat atgcgcgtgt tccaggagga gatttttggc ccggtgctgg cggtaaccac
     4021 cttcaaaacg atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc
     4081 aggcgtctgg agccgcaacg gtaatctggc ctataagatg gggcgcggca ttcaggccgg
     4141 gcgcgtgtgg accaactgct atcacgctta cccggcacat gcggcgtttg gtggctacaa
     4201 acaatcaggc attggacgcg aaacccacaa gatgatgctt gagcattacc agcaaaccaa
     4261 gtgcctgctg gtgagctact cggataaacc gttggggctg ttctgatgta agaagctggt
     4321 cgcatcgggt attaattgcc tgatgcgacg cttacgcgtc ttatcatgcc tacggggacc
     4381 tgaccgtagg ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt
     4441 acaatttacg ttttaaacct acgtgtgcaa taacccagac tacgccaaca aaagtgcgtt
     4501 tctggggcgt gttttgccag gagtttaatc tcgcgtttcg atggtgagcg ggcaaaccag
     4561 accgtaaatc accattggta ttccgaggac aatcagcaat aaacggtaga ttattgaagg
     4621 tttaatacgc ttaatctgca ttgtggatgt tttacggttt ttaatcataa atttggccaa
     4681 tagttccatt atttcaatat gataagcctc gacctgttaa aaagccagtc agcacacttc
     4741 aggaagtata taaaaaacaa tatgtcgtta tataaataag aaaattgcac tgcatcatgg
     4801 catttcataa tatgcgttaa ttgttctttt ttatattctc tgagggaaat atgttcctgg
     4861 actattttgc actgggagtg cttatttttg tatttctggt gatattctat gggattatta
     4921 ttttacatga tattccctac ctgattgcta aaaaacgtaa tcatcctcat gccgacgcca
     4981 ttcatgttgc tggttgggtg agtcttttta cgctgcatgt tatctggcca tttttgtgga
     5041 tttgggccac gctttaccgc ccggagcggg gatggggaat gcaaaaccat gactcctccg
     5101 ttgtgcaact acaacagcgt attgccgggc tggagaaaca gctcgccgac atcaaatcct
     5161 cttctgccga gtaatactta tggatttact gattattttg acctatgtgg ctttcgcatg
     5221 ggcaatgttt aagatcttca aaattcctgt aaataaatgg accattccca cagcggccct
     5281 ggggggcata tttattgtca gcggtttaat tctgttaatg aactataacc atccgtatac
     5341 ctttaaagcg caaaaagcgg ttatttctat tcctgttgtc ccacaggtga caggcgtggt
     5401 aatagaagtg acggataaga aaaatacgct gattaaaaaa ggtgaagtgc tatttcgact
     5461 ggacccgacg cgttatcagg cgcgggtgga tcggctgatg gcggatatcg ttaccgcaga
     5521 acataaacag cgggcgttgg gcgcagagtt agatgagatg gtggcgaata ctcagcaggc
     5581 aaaggccacg cgggataaat tcgctaaaga gtatcagcgt tacgcacgcg gcagccaggc
     5641 gaaagtaaac ccgttttcag aacgcgatat tgatgtggca cggcaaaatt atctggcgca
     5701 ggaagcctcc gtaaagtcat cggcagcgga acaaaaacag atccagagcc agctggatag
     5761 cctggtgttg ggtgaacatt ctcaaatcgc cagcctgaaa gcacagctcg cggaagcaaa
     5821 atataacctt gagcagacga tagtgcgtgc gccgagcgat ggttatgtga cccaggtact
     5881 gattcgtccg ggtacctatg ccgcgtcgct gccgctacgt ccggtgatgg tgtttatacc
     5941 cgatcagaaa cgacaaatcg tggcgcagtt ccgtcagaac tccttgctgc gactggctcc
     6001 tggcgacgat gcggaagtgg tgtttaatgc tctgccaggt aaggtattca gcggtaagct
     6061 ggcagccatt agtccagctg ttcccggcgg agcttatcag tcgaccggca ccttacagac
     6121 gttaaacaca gcgccgggtt cagatggcgt tatcgcgacc attgaactga atgagcacac
     6181 tgatttgagc gctttaccag acggtattta cgcccaggtg gcggtctact ctgatcattt
     6241 cagccatgtc tcggtgatgc gcaaagtact gttacgcatg accagctggg tgcattacct
     6301 ttatctcgat cattaatctt cgctttccgc cgcttgttca ggccgcagtt gctgcgactg
     6361 agcaagctgg tggcgaattt cacgcagcat ttcatccagc acatactgga cccgccaggg
     6421 ctggtattcc cgtttacgga agatagccac cagatccagc caccattcat acgggtgctg
     6481 aaagcagggc accagcgtgc cgtcttgcag gtcgctctgc acacttttgt ctggcgcaaa
     6541 cacaatcccc aggtgatttc gtgccagctc cagcgcagat tgcgtgttgt cgcaaacgta
     6601 atttcccttc acgcgataat cccgcacctc tttactcccg gcgacgttga agcgccagat
     6661 attcgcgtcg tcgatcatca tcgagtcgat caaaatacag gagtgatgtt cgagttcgtc
     6721 aggacggcta ataggatgtt tctcaagata gcgctggctg gcataagcgg ttacggcata
     6781 ttgggtaata aaactggcaa ccagcgattc atcttttggt ggggcatagc tgattaaaac
     6841 atcacaatca tcaggaaatt cgacgccttc ataaaaggca ttacgctcaa gattgcaggt
     6901 ttttagcgat aaggtgatat caccgatatc tttaatttta tcgattacat gtttggataa
     6961 ataggtaatt atgccggttg gggcgtagat ggtgacccga ccacgtttct catgcttata
     7021 atctgcaata aaattattaa gctgctcgtt tctgtccagc atgtcgttga tgtacggtaa
     7081 tagcgcggtg ccaaaaggtg tgagcatcag ttgtcgggtg gttcggtcaa aaacttttaa
     7141 acccactttt gattcaaaat cagcaagata tttgctgacg ttggcctgtg cgatgccaag
     7201 tacagtcgcg gcatggctga tattttcact ggcagcgatt accgagataa tttttaactc
     7261 ccggtactta agttgtaatt ttgtcatcgc aacagcccaa ttatatataa ttttatatat
     7321 tactatataa atatgaatct tgcaccggag aaattgtaag cattactatg ccgactcttt
     7381 tttatcttat tttaaaatgg aataccggac atgttaatta accgcaatat tgtggcgtta
     7441 tttgcgttac cttttatggc aagcgcaact gcttctgaat tatccattgg tgctggtgcg
     7501 gcttataatg aatcgcctta tcgcggttat aatgaaaata caaaggcaat tccgctgatt
     7561 agctatgaag gtgatacttt ttatgttcgt cagaccacgt taggttttat tctgtcgcaa
     7621 agtgaaaaaa atgaacttag cctgaccgca tcctggatgc cgctggaatt tgaccctgcc
     7681 gataatgacg attatgccat gcaacagctt gataagcgtg atagtacggc tatggcgggg
     7741 gttgcctggt atcaccacga gcgttgggga accgtgaaag cctctgcagc tgcggacgtt
     7801 ctggataaca gcaacggctg ggtgggggag ctatcggtat tccacaaaat gcagataggt
     7861 cgtctgtcgc tgacacctgc gctgggcgtt ctctattatg acgagaattt cagtgactat
     7921 tactatggca tttcagagag tgagtcccgt cgtagcggtc tggcaagtta ttccgcgcag
     7981 gatgcctggg tgccctatgt cagcctgacg gcaaaatacc cgataggaga gcacgtcgta
     8041 ttgatggcga gcgcaggata cagcgagctg ccggaagaga ttaccgacag cccgatgatt
     8101 gatcgtaatg agagttttac ctttgtcacc ggcgtgagct ggcgttttta attcaccggt
     8161 ggatgtcggt gcggctggcc tacgctctcg caccgacttg tatgcctgat aagacgtgtt
     8221 aagcgtcgca tcaggcaatg tgctccttac gttggatgcg gcgcgagtgc cttatccaac
     8281 ctacggatgg caatgctttt cctcattcac ccagatccgc atttcgcctt caccccggtt
     8341 ggcccagcta aaccacggga taaaggtcag agtttgcgtc tggcgtttgg ctggcgcgct
     8401 gtcgtaatgc cacagcggtt gctgctctgg attgctctgt tcataccggt aacccggtgc
     8461 ctggattaag atcttatggc taaacaatcc cttgccttca aatgtcgtaa atggtgcatc
     8521 ggtgggcagc cacagattat gcagtgactc gccgttgtcg gcctgttcca ggcaatacac
     8581 cagcgggccg cgctgaatcg ccacttttcc ggcgacgtga cgcaccagcg ggttaccgta
     8641 aacgcggcgt accggcatcg gcaaagtcag attcagcgta tcgccctcct gccattcgcg
     8701 ggtaatgtgc aaataccctt tacgaatatc ctgctcgacc tcttccccat tcaatatgat
     8761 ctgcggctgt gtgcaccagt ccggcagacg taaagccagc gtatgacgta ccggctgggg
     8821 cgattcaacc gcaatcgtca cctgctcctg ccacggatag ttcccgctaa cccgcaggcg
     8881 cagcgtgcca ttttctaccg gcacttccat gctgtttcct gcgtatatgt tgatatacaa
     8941 cgcatcttca cgcggcgtgt agagataatg accaatcgag gtcagcacgc gggcgatatt
     9001 tggcggacaa caagcgcagc caaaccaacg ctggcggatc ggtttaacgt gatcgtagat
     9061 atggttgaat ttcagcgatt ttggatgtac ttccagcgga ttcacataga agaaatgttt
     9121 gccatccagc gccatgccgc cgagcacggt gttgtacagt gcgcgctcca tcacatcggc
     9181 atattgactg tcgccttcca tttccagcat tcgtcgggca aacatcatca ggccgatgga
     9241 ggcgcaactt tcagcgtata ccgtgtcatt cggcagatcg taatcgctag tgaacgcttc
     9301 gccgctgctt tgcgagccaa tgccgccggt aatatataac tgacgctggg ccatattgtt
     9361 ccacagccgc aggcagtcct gacgcttgct gtcatcgtga cttaaacgcg cgagatgcgc
     9421 gacgccggtc atcagataga caaagcgtac tgcatgtccg atggcggttt gttgctgtgc
     9481 gatgggcaaa tgcgcctggc tgtaggcttt atctttcacc atccacgccg ggccgtaggt
     9541 gtgccagtgc gaggtctgcc cgcgcttttc atattcctgg tcgtaatagt gcggttgcgc
     9601 accacgctgt tcgacaaaat agttcgtcag tgccaggtag cgcggctctt cggtcacttc
     9661 atacagacgc atcagtgcca gttcaatttc cgggtgacca gggtaaccgt gtaacttact
     9721 ttcatctgga ccaaatacgc tgtcgatatg atcggccaga cggcaaacca cttccagcaa
     9781 gcgccgcttg cccgtggcct ggaagaaggc gactccggct tcaatcagat gaccggcgca
     9841 gtaaagttca tgacactccg ccagattgct ccagcgttct tcgggtactt ttaccgtaaa
     9901 gtaagtattg agatagccgt cttcgcattg ggcggaggcg atcagttcga ttacctcgtc
     9961 ggcggttttt tccagttcgg cgtccggctt ctggcacagc gaccaggcta ccgcttccag
    10021 ccatttggcg acgtcgctgt cctgaaacac catcccgtaa aattcaccct cctgaagtcc
    10081 ggcggcaata cgaaagtttt caatcgcatg gctgggttcc gcttctggga tacgatcgtt
    10141 caaggcatcc cactgataag gaatcaccac gtcgcggacc agttgttggt actgaccgag
    10201 gaacggatcg ctgaccgtca gtttatgcag atcgacttcc gaaatgttca tcttgtgctc
    10261 cttaaggctg tacatgacgc tgacgcaggt cggtagctat gcgtgacatc ataggattat
    10321 tgagtttgca aaagcggatc gcccctgcca gcagcaggtg gaatagcgca ggcagtaatg
    10381 tttccatcgc cgcgatcccg ccaagcgaac tggcggtctg gttttccacg ccaggctggt
    10441 aggcgacaaa aataaacagc aggctgatga tcccggcgct ggaggcccag gccagcttga
    10501 tgaaaaacag attgaaggcg aagttcatgc cggaagaacg tacgcgggtt ttccactcgc
    10561 cgtagtcatc ggcaaaggcc atcaatgaga agtgcagtgg caatgtgaag ccaagaatta
    10621 cgccattacc gaggatcacc gccagccaca gcgtttgata agccgggccg gagggcagga
    10681 accacatcaa caccgccagc gcagcgagga gcaggttggt gtagtagtaa attttgacgg
    10741 tatcgaaacg ccgcgttaac gggctgacaa tcaccgagcc gataatggag gcgaaggtga
    10801 ccatggtgaa gaacagcgac gtgtagcccg tactgccttg taagacgtag gtaatgaagt
    10861 acatataccc accgccgcga atgttaaaga cgttaatcag caggaaagac atgaccagca
    10921 tcagcagcag ttggtcgttg ttccgcagcc cggcaagatg ttcgcgcagg gtaaatttcc
    10981 ccattgtgga gagcggcacc cgttcacgaa cccagaagaa acagcacagg aacatcacca
    11041 cggcaatggc gcacaatacc ccgacgccca gttgatagcc gcgtgcagcg ttgccctgac
    11101 cgaagagatc taccatccac ggtaagccta ccgaaaccag aaatcccgct acgccgcaga
    11161 gaacgaatcg ccaggactgg caggagatca cttcattgtg gcgggtggtc atggtgttga
    11221 tcagcgcgca gtacggtacg ttgatggcgg tataaccgac tgaaaggagc aggtaagtac
    11281 cgaacgccca ggcgattttt actcccatgc tggcatctgg cacggtgaac gtcaggatgc
    11341 cgataatgcc gatgggaatg gcaatccata gctgccacgg acgaaagcgc ccccatcggc
    11401 tttgcgtccg gtcggcaatg acgcccatca ccgggtcgga gatggcatca aacacgcgca
    11461 gtgcaataaa cagcgtgcca accagcgccg gagttaatcc aaaaacgtcg gtgtagaaaa
    11521 aggtgagaaa attcatgatc aggcaagtta ttacggtgcc gcctgcgtca cccaggccgt
    11581 agcctatttt ttcccgtaca gataacgcat cgtcaatttt acctttagcg atatcagcgg
    11641 tagtaatcgg tgtagaggtc atagatattc ctcgtgaata gggagcaatt tttagtttta
    11701 ttgaaatgac ctgattaatt ctgccgtgaa cgggaatatc aacaaggcga gaaaagagta
    11761 aaaaaaggtg acgattccga cctgaattta aaagtgtgaa atggatcagg tgaaattaat
    11821 gtttttgatt aataatcaaa aggtaatgtg tttaatcatt aatgaataag cgggaaatta
    11881 catatcaatg ctcgaattat ccatgacgtt accgattaaa gtgcaaaatg gtggattatt
    11941 tatttcccgt ggcgtaggga ggcatcctgc aaggcggctg gagtcatggg aaattatctt
    12001 tgtcgaaaaa ggtcgattaa cgattcagga agaagaacgg atatttctgg tcgatgcggg
    12061 agagagttta ttactctggc cgaaccgaca acatgttggg gtggaggatt ttcccgccga
    12121 tctcaaattt tactggctgc attttgaggt taaagcgccg gacaacgacc ctcgctggct
    12181 tactcatctt agcgtgccgc aacaaacgaa ggttgtcgat ccgcaggcgc tggtggccct
    12241 gtttcgccag ttcttaagcg aacaggaaaa gcatcaacgc actccggcgc tggagtttat
    12301 tgtgctgctc attttgcagc agctcacccg tgacgcgcgt cttgatgaac aggccgatgg
    12361 cgcaggcacc acccttgcct ggaaagcgca gaaactgatc cgcacgcaat atcatcgccc
    12421 gctttctacg gcgcttctgg cccgcgagct gtactgcaat gccgattatc ttgggcgcgt
    12481 ctaccggcgg gtgtttcatc tgacgttaac agaagcaatc catcgccagc gggtgcgcga
    12541 agccgaaaag ctgttaatta gcgaagcgcg atcgctcacg gaagtggcgg cgttgtgcgg
    12601 gtttaatgac gttggctatt tccggcagat ttttcgcaaa cacaccggtt taacgccagc
    12661 agcgtggaag cggcgttaca gcaaggaaca tatcaattcg tagtgccggg gcgatttcgc
    12721 tttcgacatc acgtctgaaa caggtgggag agaaaaatct cctgaaacct ctgcgtgaaa
    12781 ccgcgcaggc tatttctaat gaactggggt ttactgtcag ggacgaccag ggcgcgatcg
    12841 cataacgctt tggacaagtg ccaaaacttt aacatttcct tcgttggatc aaagcagtag
    12901 gggcgcgttc tctggcactc tgctgtttta gtgcaaaggg gtgatcatga accggtttat
    12961 tattgcggat gcgacgaaat gtatcggttg ccgtacctgt gaagtggctt gcgcagtgtc
    13021 gcatcaggaa aatcaggatt gcgctgcgtt gtcaccagac gagtttattt cccgtattcg
    13081 tgttattaaa gaccacagct ggaccacggc agtagcctgt catcagtgtg aagatgcacc
    13141 gtgcgcgaat gtctgccctg ttgacgcgat aagccgcgaa catgggcata ttttcgttga
    13201 acaatcacgt tgcattggct gtaaaagctg tatgctggct tgcccgtttg gtgcgatgga
    13261 ggtcgtttct tcgcgcaaaa aggcgagggc gattaagtgc gatctgtgct ggcatcgggc
    13321 gacgggaccg gcctgtgttg aagcctgccc gacaaaggcg ttgcagtgca tggatgtcga
    13381 gaaagtgcag cggcatcggc tacggcagca gcctgtttga aacgttttcc ggcgtttctc
    13441 aacgccggaa tagtgcgctt tgtttatgcc agatgcggca taaacgcctt atccggccta
    13501 caaattcttc caaattcaat atattgcaga aatcatgtag gcctgataag cgtagcgcat
    13561 caggcaattt ttagtgactt tcagcccagg ctctttctat ctcttccgcc agaatcttca
    13621 cccccgcctc aattttctcc ggctctggta cgtagttcat gcgcatacat tgatgcgtat
    13681 gcggccacgg tttatccagc cctgggaaga agttgtgccc cggcaccatc agcacgccgc
    13741 gtgctttcag gcgctgatag agctgctcgg tcgtaatggg caaatcctta aaccatagcc
    13801 agaggaaaat ggctccttct ggtttatgaa tcaggcagcg atcttccggt aaatagcggc
    13861 gaatgatggc gatagtttcc tgaacacgct ggtagtaaaa cggtttgatg actgtttcag
    13921 acatgcgcag cagatcgtta cgcttaatca tttcacacat catcgccgga ccaataccgc
    13981 caggtgccag gctgataatg ccgttcatat tggtgatggc ggtgatgatt ttttcattgg
    14041 cgatgataat gccgcagcgg gagccaggta gacccagctt ggaaagactc atgcacagca
    14101 cgatattcgg attccatagc gggcgcgctt cactgaagat gatacccgga aacgggacgc
    14161 cataagcgtt atcaatcacc agcggaatgc cgtgttgatt cgccagcgcg tcaagcttca
    14221 gcaactcttc gtcagtaatc acattgcctg ttggattcgt cggccgggag acgcaaatca
    14281 tcccggtttc ttcgccaata tgcagatgct caaaatcgac gtggtattta aactggcctt
    14341 caggcagcag ttcaatattc ggacgcgcag agacaaacag atcttcttcc agtccggcgt
    14401 cagcgtagcc aatgtattcc ggtgccagcg ggaacagcac ttttttgacc cgaccatcgg
    14461 cacggcgtcc ggcaaacagg ttaaataagt agaaaaacgc gctctggctg ccgtttgtta
    14521 gtgcaatatt ctgtggttcg atatcccaac ccaacttctc gtgcagcatt ccggcaagca
    14581 gtgtgagtag ctccgttttc ccctgtggac cgtcgtaatt gcacagtgca tcagtcgctt
    14641 tgccactttc cagcatgtcg gtcagtagcg tctggaagta gtcctgcatt tccgggatct
    14701 gcgccggatt accgccgccg agcataatcg cgccaggcgt gcgtaaaccg tcgttcagat
    14761 cttccatcag cagcgtaatg ccggagtggc gggtaaattt gtcaccaaaa agggagaatg
    14821 tcatagcggg atatctgtcg aactctgtag caaggaaggt aacaataacg ctacatttgc
    14881 tggggtgcaa atcggtctgt tgaagggtga gcggtacttt atcctgcaac gttaattagg
    14941 gatgacattt tatcctggtt ggcggcttcc cgaggtactg acgaacggga agccggaaaa
    15001 gttactgttg ccctgcccag acgaccagca ctttatcgtc gccatgctca cgaacaaagc
    15061 cgtagccctg cttcagcgaa agtgtcgttt gtttgcccgc gccaattgcg ggatgacggg
    15121 cgcggaactg gctgattttc tgccagtgcg cgacgctggc ggcagattta ccgctaacat
    15181 cctgccagtt catatccgaa cgcgtacctt gcagcggatc agaaccggta ggaccgaacg
    15241 gacgcgagga ttcatcacca taaaagattt gtaccgcgcc tggcgctaat aataataact
    15301 ctgctgcttt gtcgccccct tcacggaaca ggcgggtatc atgcgacgag aggtagctca
    15361 acacgttgaa accctgcaat ttctccgcca tttgctgcca ggtcgtatcc atctgcgcca
    15421 gacaatcgac tgctttcgcc gcctgctcct gataatcgaa attgatcatc gcatcgaagc
    15481 cgtggcgata gtagtcactt tgcatcacgc cgtggcccca ggcttcaccg gtcatccaga
    15541 aaggtttgtc atctaatgct ttgtcggggt tagctttttt ccattcgcga agcgcggcgc
    15601 tggcttcggt tttcagttgc tgccaggcgg gcaactcaac atgtttggcg gtatcgactc
    15661 gaaaaccatc aatcccatag tcgcggaccc actgacttaa ccagtgggtt aagtaatcgc
    15721 gaggggtaaa gccgtcgatg actttagcgt gggtatccgt tttgttttta tagaacaccg
    15781 gcagaccaga agcggtcgtt gattcggttt tgatatccgg caaaaaggcc agcgacatag
    15841 tgagatcgtc aaatccagga ttgtcgtaat cgccgatatc cgttctgatc cagttttttc
    15901 cccaccattt atcccagcct gttttgtcgc tgaaattaat gtaatcgtta aagctatgcc
    15961 aggtttgccc ggcggcaggt ttccagtcac tccagcgttc acccagcgtt tttttcagct
    16021 cgtcaccaga aagatataac gcgccaaact gatactcctg catatccgcc agcgtggcat
    16081 agccggtgtg gttcatcacg acatcaaaga gaatacgaat accgcgctga tgcgcgctat
    16141 caaccagcgt ccgtagatcg gcttcgctgc ccatattggc atcaagattc gtccagtcct
    16201 gtgtgtaata accgtggtag gcataatgcg ggaaatcacc ttttgtaccg ccgccgaccc
    16261 agccgtgaat ttgctcaaat ggggcgctta tccacaaagc attgacgccc agctgctgga
    16321 gataatccag tttgttggtc aggccgcgta aatcgccgcc gtgaaaagta cctatttctg
    16381 ccataccgtc tttatgacgt ccgtaactct ggtcattact gggatcgccg ttttcgaaac
    16441 gatctgtcag cacaaagtaa accgtggcgt tatgccagtc gaaaggggca gaggcgtcag
    16501 tttctgcccg ttccagcagg agtaaaccgt tgctggtggc agcgggttgt aacgttattt
    16561 gaccgttctt cactatcgca atttgctggc tgtaataatc ccgtacggcg gctccttccg
    16621 ggaaagtggc gctgacatcc actgtgagcg gtaatccatc ccatttcggg cattcacgga
    16681 ccaggcttgc taccggttcg gcggcgttct ggatggaaat catcaatgtt ggcgtaccgg
    16741 agcgggtgtc tatttgcagc gtatattcgc cgtccctgaa caatcgccat tgaggcggcg
    16801 tgttgctaca aggttgcagg gaaagcatct gattgagttt tatcacatcc gcaggctgcc
    16861 agcactgttg gtcaaaattt agcgttagtg gacgcgtacc tttgggcaac tgcgcgtggc
    16921 tgacaaatgt tcccgttccc tgttcactaa aggcagggaa ccccggagaa gtccagctgg
    16981 cggcaacggc gaagccagga aggagtgtca gaaaacaggc ggcgagtttc atggcggatg
    17041 agtccttatt gctgcgattt cagacagttt gccagcgata agccaaacaa aactcatccc
    17101 tgagcggtaa gtccacagga tgagaagcaa ggttgagcga tctcgcgcaa aaaacggctg
    17161 aattttgcga taaaccccac attttctgcg atttagcgcc aatctgaatc gttaacacgt
    17221 gacatagttt cagatttgga ctattccttg gggtgctctt gacagctatt tttgatatga
    17281 tttgagattc aactctcaaa tttgtgaaaa atataaggtg ttggaatgat taaatccgac
    17341 caggagacct gatgattttg actcccatac gacgatatgg ggcgatgatt cttatgttac
    17401 tcactctggt gttttcgagt gaggtgttag cgaagacgca cacaacaaca gcgagtcaaa
    17461 agtcccactt aactaaagct agtaataaac aggtaagcag taaacaagag tattctcgca
    17521 atagtgcaaa gagtagttca cttcctgatt tgcgaaaata cccttccgga acaccaagga
    17581 aaaaggcgtt tctccggacc gtaatgcctt acattaccag ccaaaatgca gccattactg
    17641 cagaacgtaa ctggctgatt tcaaaacagt atcagggcca atggtcacct gctgagcgtg
    17701 cgcgtctgaa agacatcgcc aaacgctaca aggtgaagtg gtctggtaat acgcgaaaaa
    17761 tcccgtggaa taccttgctt gaacgcgtag acattatccc taccagtatg gtggcgacga
    17821 tggctgcagc agaaagcggt tggggaacgt cgaagctggc gcgcaacaac aacaacctgt
    17881 tcggcatgaa atgcatgaaa ggacgttgta ccaatgcgcc aggtaaagtg aaagggtact
    17941 cacagtttag ttctgtcaaa gaatcggtga gcgcctatgt cactaacctg aatacgcacc
    18001 cggcttactc ttcgttccgt aaatcgcgtg cgcagctgcg taaagctgat caggaagtga
    18061 ctgccacagc gatgattcac aagctgaagg gctactcgac caaagggaag agttataaca
    18121 actacctgtt cgcaatgtac caggataacc aacggttaat cgcggcgcat atgtaattgt
    18181 atttcctcga gccttatccg acttgtcggt cggataaggc ttttgatcgc atcaggtggt
    18241 tgtgctgagt tacctgatgc gacgccgaca attctcatca tcgctacaac atgacctcgc
    18301 tatttacatc gcgatactct tttggcgtcg tgtcatatgc ttttttaaaa acagagtaga
    18361 aatattgcag cgatggataa ccgcacattt gcgatatctc attgatcgac aaggtggttg
    18421 aaatcagcag actgcgcgct ttctccagct tctcggcatg aatcatggca tggatggttt
    18481 cacccacctc ttctttaaaa cgcttctcaa gattggagcg cgagatcccg accgcatcga
    18541 gtacctgatc cactttaatc cctttacagg cgtgattacg aatgtaatgc atggcctgaa
    18601 taacggcggg atcggtcagc gaacggtaat ctgttgagcg ccgttcaatg acgcgaactg
    18661 gagggaccaa aatccgctgt agcggcattt cttctttatc taataatcga tgcaacagtt
    18721 ttgccgcctg atagcccatt tgccgcgcgc cctgagcgac cgaagaaagg gcgacacgcg
    18781 acagatagcg ggtcagttct tcgttgtcga tgccaatcac gcataatttt tccggtacgg
    18841 gaatgtgtag atgttcacat acttgcagaa tatgccgcgc tcgggcgtca gtaacggcaa
    18901 taatcccggt ttgcggtggc agcgtttgta gccagtctgc cagccgattt tgcgcgtgtt
    18961 gccagttctc tggcgcggtt tctaacccct gataaaccac tccgcgatac ttttcttcgg
    19021 cgacaagctg acgaaatgca tattcgcgtt cagtggccca acgtttgccg cttgattccg
    19081 gaagaccata aaaagcaaag cggttaacgc ctttctcttt taaatgcaaa aatgcgcttt
    19141 caaccagcgc atagttatcg gtggcaatgt aatgaacggg tgggtaactt tctgcaaggt
    19201 gatacgagcc gccaacccca acaatgggga cgtcgacatc agccagcgct tgctcgattt
    19261 gtttgtcgtc gaagtcggca atgacgccat ctcctaacca gtccttgatt ttatcaatgc
    19321 gggcgcggaa atcttcttca atgaaaatat cccattccga ttgtgacgcc tgtaaatatt
    19381 cccctacgcc ctctactacc tgccggtcat aggctttatt ggcattgaac agtaatgtga
    19441 tgcggtgacg tttagtaaac atggttcttt tcctgctgaa tcatgcaaaa actcaaaacc
    19501 ggtaatacgt taccggcttt gagaaaattt ttatcaaaat caagaacggc gtttggttgc
    19561 ggagtccatc catactgcca gcaacagaat cgcaccttta acgatatact gccagaaggt
    19621 cggtacatcc atcatactca tgccgttatc cagtgaagcc atgataaatg cccccattac
    19681 cgctccagcc acgctcccga caccgccagc cagactggtg ccgccaatca cgcatgctgc
    19741 aattgcgtcc agctcggcga tattccccgc agaaggtgaa ccagcgccaa gtcgagaact
    19801 gaggattaat ccggcgatgg ctaccattaa tccgttaata gcgaacacgg caagtttggt
    19861 gcgttcaacg ttaatcccgg agagacgtgc tgcttccaga ttgccgccga tggcataaat
    19921 gcgtcgtcca aatgccgtcc gcgttgccat aaacattccg ccgagtaaca gcaacgtcag
    19981 cagcagaaca ggagtgggaa cgccacgata atcattcaac agccagattg cgcctaatac
    20041 gatgatagcg gttaaagcct ggcgaccgac tactgcggta gaggccggag actgcaaacc
    20101 caaagcctga cggcgcattc ttccgcgcca ttgccagcca acaaaagcca ttaagccaag
    20161 cgcgccaatg atgaagccgg tgctggcggg gagatagctt tgcccaattt gtgacatcgc
    20221 ggcgctggtg ggggatacag tcgtgccgtt ggtgatgcca atgagtatgc cgcgaaatgc
    20281 caacatgccc gcgagagtga caataaatga agggactttg cggtacgcga cccaccatcc
    20341 gttccaggca ccgagaagca gtcccagaac caacgtcaca atgatggtaa gtggcaaagg
    20401 ccagcctagc cagacgtcac aaatcgccgc gacgccacct aatagcccca tcattgagcc
    20461 gacggaaagg tcgatttcag cagaaattat gacgaacacc attcctaccg cgaggatgcc
    20521 ggtaatcgcg gtctggcgta acaggttgga gacgttacgg gcgcttaagt aggcaccatc
    20581 ggtggtccag gtaaagaaca gcatgattgc gatgatagct gcaatcatca cgaagacctg
    20641 caaattcagt gatttcagcc cggagaagcc accggatgtc ggtacggcca atttcacttc
    20701 agacggattg cttttcgaca tgatgttcgc tcctcaatgc ggcttccatc acctgctcct
    20761 gagtcaggtt atgatttatc aggttggctt ttagtttccc ttcatgcatc accagtacac
    20821 gatcgctaag gccgagcact tcaggtaatt cggaagagat gacaataacg gcaataccct
    20881 gctggacgag ttggttaatt aatttgtaga tctcatattt cgcgccaata tcaatacccc
    20941 tggtaggttc atcaagaatg agaatgcgcg ggttgagtaa cagacagcgg gcgaggatcg
    21001 ctttttgctg attgccgccg ctcaaacgtc caatagcaag gtcgggggac gacgttttaa
    21061 ctttgagttg ctggattgat tccagaatac atttttgctc tgccgcgtca tcaagctggc
    21121 taatgccacc ggtaaattta ttgagtgcgg cgagggtaat atttttacca accgccatta
    21181 ccggaacgat gccgtcgcgc tttctgtctt cggggaccat cgcaatcccc tgggcgatgg
    21241 cttgctgaca gttacgaata tctacctgtt tgccatcaat ataaattttt ccttcccatt
    21301 gtccgggcca cacaccaaac aggcactgaa tggtctcggt acgtccggca ccaacgagtc
    21361 cggcaatacc cagtatttcg ccacgtttca gggaaaacga gacatcatta actcgtttaa
    21421 tatgacgatt aaccggatgc catgccgtca gatgttcaat acgtaatatt tcatctccgg
    21481 tggtatgtgg ttcattaggg taaagcgcgg ttaactctcg cccgaccatc atggtgataa
    21541 tatcgtcttc actcattccg gcagcattac gcgtaccaat gtgctgtccg tcgcgaataa
    21601 cgcaaatcgt atcggaaatc gctttgactt cgttgagttt gtgcgaaata taaatacagg
    21661 cgataccgtg ctgttgtaga tcgcgaataa tatccagtaa aaccgacgtt tcctgctcag
    21721 ttaatgaggc tgtcggttca tcgagaatta acaagcgcac ctgtttatta agtgccttgg
    21781 caatttcaac cagttgttgt tgcccaagcc ctaaatcgcc aacgcgggta tcaggtgaaa
    21841 tggataaact gacctgtgcg agcagcttct gacagcgtag cgtcatcagg tcataatcca
    21901 taatgccatt gtgggttatt tcgttaccca ggaagatatt ttccagcacg gtcaattctt
    21961 tcaccagggc caattcctgg tgaatgatgg cgataccttt gcgttcggta tcgcggatgt
    22021 ggctcgcctg aatctcttct cccgcaaaaa taatttcgcc ttcgtaggag ccatggggat
    22081 aaataccaca cagcactttc atcagcgttg atttaccaga cccattttcc ccacagagtg
    22141 agacgatttc gccagcattc aaccgcaagc tgacgttatc aatcgccttc acactgccga
    22201 aggttttggt aatgttcttc atttcaagta gataaggcat aacgactcca cctaagccaa
    22261 ttcattcacg cggcatggag agaaatcacg cccccgctcc gcgctggggc gtaacgctta
    22321 cagctcgctc tctttgtgga atccgtcttt aattaccgta tctttgatgt tgtttttatt
    22381 cacatcgatc ggtgtcagga ggcgggaggg cacatctttc aggccattat tcagtgtggt
    22441 atctgctttt ggttcctgac cattgcccaa ctcaacggca atttctgcgg cagtatttgc
    22501 caacaacgta ataggtttat acaccgtcat agtttgcgta ccggcagcaa tacgtttaat
    22561 acctgcgaga tccgcatcct ggccggaaat tgctactttc cctgataaac cttgcgcgct
    22621 taatgcctga attgccccac ctgcggtggc atcgtttgag gcaactacag catcaatttt
    22681 gttattattg gcggttagcg cgttttccat aattttcaat gcgttttccg gtaaccagcc
    22741 atcaacccat tggtcaccaa cgactttaat ttttccggaa tcaacgtaag gttttaacac
    22801 tttcatttgt ccggcgcgga acagcttggc gttgttatct accggcgagc cgcccatcag
    22861 gaagtaatta ccttgtggaa caatatcgac cagggctttt gcctgcagtt caccgacttt
    22921 ttcgttatcg aaagaaatat aaaaatcgat atccgcatcg ttaatcatac ggtcgtaagc
    22981 taatacttta atgccttctt gtttggcttc ttttacaacg ttacttaata cctgaccgtt
    23041 atacggaata atgacaagaa catcgacacc ccggtttatc atgttttcaa tctgcgacat
    23101 ttgtgtttct tcattgccat ttgcagactg tacaaatact ttcgcgccga gagattctgc
    23161 ctttttcaca aagatatctc gatctttttg ccagcgttca agacggagat catcaatcgc
    23221 catacctatt ttgacttctt tggcgtgtgc agcaacgttg gtaagcagga gtgaggtgca
    23281 aagggtgagt agaatgttct ttattttcat ggtgtagggc cttctgtagt tagaggacag
    23341 ctttaataag taacaatcac cgcgataaac gtaaccaatt tttagcaact aaacagggga
    23401 aaacaattac agatttttat ctttcgatta cgatttttgg tttatttctt gatttatgac
    23461 cgagatctta cttttgttgc gcaattgtac ttattgcatt tttctcttcg aggaattacc
    23521 cagtttcatc attccatttt attttgcgag cgagcgcaca cttgtgaatt atctcaatag
    23581 cagtgtgaaa taacataatt gagcaactga aagggagtgc ccaatattac gacatcatcc
    23641 atcacccgcg gcattacctg attatggagt tcaatatgca agcctatttt gaccagctcg
    23701 atcgcgttcg ttatgaaggc tcaaaatcct caaacccgtt agcattccgt cactacaatc
    23761 ccgacgaact ggtgttgggc aagcgtatgg aagagcactt gcgatttgcc gcctgctact
    23821 ggcacacctt ctgctggaac ggggcggata tgtttggtgt gggggcgttt aatcgtccgt
    23881 ggcagcagcc tggtgaggca ctggcgttgg cgaagcgtaa agcagatgtc gcatttgagt
    23941 ttttccacaa gttacatgtg ccattttatt gcttccacga tgtggatgtt tcccctgagg
    24001 gcgcgtcgtt aaaagagtac atcaataatt ttgcgcaaat ggttgatgtc ctggcaggca
    24061 agcaagaaga gagcggcgtg aagctgctgt ggggaaccgc caactgcttt acaaaccctc
    24121 gctacggcgc gggtgcggcg acgaacccag atccagaagt cttcagctgg gcggcaacgc
    24181 aagttgttac agcgatggaa gcaacccata aattgggcgg tgaaaactat gtcctgtggg
    24241 gcggtcgtga aggttacgaa acgctgttaa ataccgactt gcgtcaggag cgtgaacaac
    24301 tgggccgctt tatgcagatg gtggttgagc ataaacataa aatcggtttc cagggcacgt
    24361 tgcttatcga accgaaaccg caagaaccga ccaaacatca atatgattac gatgccgcga
    24421 cggtctatgg cttcctgaaa cagtttggtc tggaaaaaga gattaaactg aacattgaag
    24481 ctaaccacgc gacgctggca ggtcactctt tccatcatga aatagccact gccattgcgc
    24541 ttggcctgtt cggttctgtc gacgccaacc gtggcgatgc gcaactgggc tgggacaccg
    24601 accagttccc gaacagtgtg gaagagaatg cgctggtgat gtatgaaatt ctcaaagcag
    24661 gcggtttcac caccggtggt ctgaacttcg atgccaaagt acgtcgtcaa agtactgata
    24721 aatatgatct gttttacggt catatcggcg cgatggatac gatggcactg gcgctgaaaa
    24781 ttgcagcgcg catgattgaa gatggcgagc tggataaacg catcgcgcag cgttattccg
    24841 gctggaatag cgaattaggc cagcaaatcc tgaaaggcca aatgtcactg gcagatttag
    24901 ccaaatatgc tcaggaacat aatttgtctc cggtgcatca gagtggtcgc caggagcaac
    24961 tggaaaatct ggtaaatcat tatctgttcg acaaataacg gctaactgtg cagtccgttg
    25021 gcccggttat cggtagcgat accgggcatt tttttaagga acgatcgata tgtatatcgg
    25081 gatagatctt ggcacctcgg gcgtaaaagt tattttgctc aacgagcagg gtgaggtggt
    25141 tgcttcgcaa acggaaaagc taaccgtttc gcgcccgcat ccactctggt cggaacaaga
    25201 cccggaacag tggtggcagg caactgatcg cgcaatgaaa gctctgggcg atcagcattc
    25261 tctgcaggac gttaaagcat tgggtattgc cggccagatg cacggagcaa ccttactgga
    25321 tgctcaacaa cgggtcttgc gccctgccat tttgtggaac gacgggcgct gtgcgcaaga
    25381 gtgcgctttg ctggaagcgc gagttccgct atcgcgggtg attaccggca acctgatgat
    25441 gcccggattt actgcgccta aattgctatg ggttcagcgg catgagccgg agatattccg
    25501 tcaaatcgac aaagtattat taccgaaaga ttacttgcgt ctgcgtatga cgggggagtt
    25561 tgccagcgat atgtctgacg cagctggcac catgtggctg gatgtcgcaa agcgtgactg
    25621 gagtgacgtc atgctgcagg cttgcgactt atctcgtgac cagatgccag cattatacga
    25681 aggcagcgaa attactggtg ctttgttacc tgaagttgcg aaagcgtggg gtatggcggc
    25741 ggtgccagtt gtcgcaggcg gtggcgacaa tgcagctggt gcggttggtg tgggaatggt
    25801 tgatgctaat caggcaatgt tatcgctggg gacgtcgggg gtctattttg ctgtcagcga
    25861 agggttttta agcaagccag aaagcgccgt acacagcttt tgccatgcgc taccgcagcg
    25921 ttggcattta atgtctgtaa tgctgagtgc agcgtcgtgt ctggattggg ccgcgaaatt
    25981 aaccggcctg agcaatgtcc cagctttaat cgctgcagct caacaggctg atgaaagtgc
    26041 cgagccagtt tggtttctgc cttatctttc cggcgagcgt acgccacaca ataatcccca
    26101 ggcgaagggg gttttctttg gtttgactca tcaacatggc cccaatgaac tggcgcgagc
    26161 agtgctggaa ggcgtgggtt atgcgctggc agatggcatg gatgtcgtgc atgcctgcgg
    26221 cattaaaccg caaagtgtta cgttgattgg tggcggggcg cgtagtgagt actggcgtca
    26281 gatgctggcg gatatcagcg gtcagcagct cgattaccgt acgggggggg atgtggggcc
    26341 agcactgggc gcagcaaggc tggcgcagat tgcggcgaat ccagagaaat cgctcattga
    26401 gttgttgccg cagctaccgt tagaacagtc gcatctacca gatgcgcagc gttatgccgc
    26461 ttatcagcca cgacgagaaa cgttccgtcg cctctatcag caacttctgc cattaatggc
    26521 gtaaacgtta tcccctgcct gaccgggtgg gggataattc acatctatat atctcagcaa
    26581 ttaattaata tttagtatga atttattctg aaaatcattt gttaatggca tttttcagtt
    26641 ttgtctttcg ttggttactc gtaatgtatc gctggtagat atggagatcg ttatgaaaac
    26701 ctcaaaaact gtggcaaaac tattatttgt tgtcggggcg ctggtttatc tggttgggct
    26761 atggatctca tgcccattgt tgagtggaaa aggctatttt cttggcgtgt taatgacagc
    26821 aacttttggc aactatgcat atcttcgcgc agaaaaactc gggcaactgg ataatttttt
    26881 tacccatatt tgccagttag ttgcgttaat cactatcggt ctcttgttta tcggtgtttt
    26941 aaatgcacct atcaatgctt atgaaatggt gatctacccc atcgcctttt ttgtctgctt
    27001 gtttggtcaa atgcgtttgt ttcgctcggt atgagcaaca taaagctctt atacattcag
    27061 gaatgaaagg aatactgtga tggacaacaa aatatcaacc tattcaccgg cctttagtat
    27121 tgtgtcatgg atagctctcg ttggtggtat cgttacctat ctgttagggc tatggaatgc
    27181 agagatgcag ttaaatgaaa aaggatatta ttttgccgta ctggtattag gactgttttc
    27241 tgcggcgtct tatcaaaaga ccgttcggga caagtatgaa ggcataccga ccacttccat
    27301 ttattatatg acctgcctga ctgtctttat tatctctgtt gcattactga tggtaggtct
    27361 gtggaatgcg acattattac tcagcgaaaa aggtttttat ggactggctt tcttcttaag
    27421 cttgtttggt gcagtagcgg tgcagaaaaa tattcgtgat gccggaataa acccaccaaa
    27481 agaaacacag gttacccagg aagaatacag cgaataactc acgtaagccc ggtcagtcca
    27541 atgtgaccgg gcttttactt aactcactaa tctgtttctg tcgattcgtt gtaccagcat
    27601 agaaagtaac aaactcgctg ccaacgtcgc gcaaaaaatc caaataatat ccagtattgg
    27661 ccaattttta agctcaattc cccgggtgcg cagcgcatgg ataatcaagg cgtgaaatcc
    27721 gtatataccc aacgaatggc gggagattaa gccaagtccg cgaatggtac gcgtatccag
    27781 cgtgttttta accagagtca atagcgcgat tgcgcagata aaaaccatcg gcccacagta
    27841 aagataccag gtatcggcaa aatttccgcg ccactgcaat tcatataatg tcccgcgaga
    27901 gataataaaa actcccgtcg caaacagtgc ggcgctcacc cacgacagtg ctttatgctg
    27961 tgtgtccatc atccctaaag cgcggcccaa catgccatac agaatgtagt aaaaagtatc
    28021 gccattgata tataagttaa ttggcagcca ttcaaaaccg tcgattttct gcggcacggt
    28081 gtttgggtta gcgatgatac caatcaccac cattagcacc agcaacattt ttccgccgac
    28141 gttcttcacc tgaatcagcg gtgaaaccag ataaatcacc gcaatcgcga agaaaaacca
    28201 taagtggtaa aacactggct tttgcagcag gtttttcagc gctaactcca cattaatgga
    28261 ggtaaacagt gcaatgtaga gcagcgcgat tgcgctataa aaaaacagac ataagccgat
    28321 acgcaagaaa tggcgcggct gggcgctgcg ttcgccaaaa aagagatagc cggaaatcat
    28381 gaaaaatagc ggcacgctga cacgagaggc agagttcaga acattggcga tatcccaggt
    28441 gacggggcta acactatgag cattggtcac ataccaggta gtggtgtgaa tcatcaccac
    28501 cattaaacac gctatccctc gcaggttatc aatccagtaa attttgggct gcatctgtgt
    28561 ctctgtatct ggttaaaaaa agtctgaccg ataaatcatt cagttggcgc actggaataa
    28621 tctgagtttt aacactacag cttatagagg cttaaggaaa ttcgtaagat atcagccact
    28681 ataccgatat aaataataag actcacctgc aaaccagacg gtaatttaat gatgatgaac
    28741 tctttctttc cggcaatggc gcttatggtg ctagtgggtt gttctacacc gtcacccgtg
    28801 cagaaagcac aacgggtaaa ggttgatcct ctgcgttcgt tgaatatgga agcgttatgc
    28861 aaggatcagg cggcaaaacg ttataacacc ggcgagcaaa aaatcgacgt caccgccttc
    28921 gaacagttcc agggaagcta tgaaatgcgc ggttatacct tccgtaaaga gcagtttgtc
    28981 tgttcttttg acgcagatgg ccattttttg catctttcca tgcgttaagc cctgcttttt
    29041 cccgtttcat actgtatatc ttccatccag cgggtatact gatcccttcc tttaaatcca
    29101 cacgtatcca gcacgaaata atatgcaaaa gtttgatacc aggaccttcc agggcttgat
    29161 cctgacctta caggattact gggctcgcca gggctgcacc attgttcaac cattggacat
    29221 ggaagtcggc gcgggaacct ctcacccaat gacctgtctg cgcgcgctgg ggccagaacc
    29281 gatggcggct gcttatgttc agccttctcg tcgcccgacc gatggtcgct acggcgaaaa
    29341 ccccaaccgt ttacagcact actatcagtt ccaggtggtc attaagccat cgccggacaa
    29401 tattcaggag ctgtaccttg gttctctgaa agagctgggc atggacccga ctatccacga
    29461 tatccgtttc gtggaagata actgggaaaa cccgacgctg ggtgcctggg gactgggctg
    29521 ggaagtgtgg ctaaacggca tggaagtgac gcagttcact tacttccagc aggttggtgg
    29581 tctggagtgt aaaccggtta ccggcgagat cacctacggt ctggaacgtc tggccatgta
    29641 cattcagggc gtagacagcg tttacgacct ggtctggagc gacggcccgc tgggtaaaac
    29701 cacctacggc gacgtgttcc atcagaacga agtggagcag tccacttaca acttcgaata
    29761 cgcggacgtg gacttcctgt tcacctgctt cgagcagtac gagaaagaag cgcagcagct
    29821 gctggcgctg gaaaatccgc tgccgctgcc agcttacgag cgtattctga aagccgccca
    29881 cagcttcaac ctgctggatg cgcgtaaagc catctccgtc accgagcgtc agcgctacat
    29941 tctgcgcatt cgcaccctga ccaaagcagt ggcagaagca tattacgctt cccgtgaagc
    30001 cctcggcttc ccgatgtgca acaaagataa gtaagaggcg gctatgtctg agaaaacttt
    30061 tctggtggaa atcggcactg aagagctgcc accaaaagca ctgcgcagcc tggcagagtc
    30121 ctttgctgcg aactttactg cggagctgga taacgcaggc ctcgcacacg gcaccgttca
    30181 atggtttgct gctccgcgtc gtctggcgct gaaagtagct aacctggcgg aagcgcaacc
    30241 ggatcgtgaa atcgaaaaac gcggcccggc gattgcccag gcgttcgacg ctgaaggtaa
    30301 accgagcaaa gcggcagaag gttgggcgcg tggttgcggt attaccgttg accaggctga
    30361 gcgtctgact accgataaag gcgaatggct gctgtatcgc gcccatgtga agggcgaaag
    30421 cactgaagca ctgctgccga atatggttgc gacttcgctg gcgaaactgc cgattccgaa
    30481 actgatgcgc tggggtgcaa gcgacgtgca cttcgtgcgt ccggtgcaca ccgtgaccct
    30541 gctgctgggc gacaaagtca ttcctgcaac cattctgggc attcagtccg atcgcgtgat
    30601 tcgcggccac cgctttatgg gcgagccgga attcaccatc gataacgccg atcagtatcc
    30661 ggaaattctg cgtgagcgtg ggaaagtcat cgccgattac gaagaacgta aggcgaagat
    30721 taaagccgat gccgaagaag cagcgcgtaa gattggcggt aacgctgact taagcgaaag
    30781 cctgctggaa gaagtggctt cgctggtgga gtggccggtc gttctaaccg cgaaatttga
    30841 agagaaattc ctcgcggtgc cgtctgaagc actggtttac accatgaaag gtgaccagaa
    30901 gtacttcccg gtgtatgcga acgacggcaa actgctgccg aactttatct tcgttgccaa
    30961 catcgaatcg aaagatccgc agcaaattat ctctggtaac gagaaagtcg ttcgtccacg
    31021 tctggctgat gccgagttct tcttcaacac cgaccgtaaa aaacgtctgg aagataacct
    31081 gccgcgtctg caaaccgtgc tgttccagca acagctgggt acgctgcgtg acaaaactga
    31141 ccgcatccag gcgctggctg gctggatcgc cgaacagatt ggcgctgacg ttaaccacgc
    31201 aacccgtgcg ggcctgctgt ctaagtgtga cctgatgacc aacatggtct tcgagttcac
    31261 cgacacccaa ggcgttatgg ggatgcacta cgcgcgtcac gatggcgaag cggaagacgt
    31321 agccgtcgcg ctgaatgagc agtatcagcc gcgctttgcc ggtgatgacc tgccgtccaa
    31381 cccggtagct tgtgcgctgg cgattgctga taagatggat actctggcgg gtatcttcgg
    31441 tatcggtcag catccgaaag gcgacaaaga cccgtttgcg ctgcgtcgtg ccgcacttgg
    31501 cgtgctgcga attatcgttg agaagaacct caaccttgat ctgcaaacgc tgaccgaaga
    31561 agcggtgcgt ctgtatggcg ataagctgac taatgccaac gtagttgatg atgttatcga
    31621 ctttatgctc ggtcgcttcc gcgcctggta tcaggacgaa ggttataccg ttgacaccat
    31681 ccaggcggta ctggcgcgtc gtccgactcg tccggctgat ttcgatgccc gcatgaaagc
    31741 ggtatcgcac ttccgtaccc tggaagcagc tgcagcactg gcggcggcga acaaacgtgt
    31801 atctaacatc ctggcgaaat ctgacgaagt gctgagcgat cgcgtgaatg cctctactct
    31861 gaaagagccg gaagaaatta aactggcgat gcaggttgtg gtgctacgtg acaagctgga
    31921 gccgtacttt gcggaaggtc gttaccagga tgcgctggtc gaactggcag agctgcgtga
    31981 accggtcgat gccttcttcg ataaagtgat ggtcatggtt gatgacaaag aattgcgtat
    32041 caaccgtctg accatgctgg agaaactgcg cgaactgttc ctgcgcgttg cggatatttc
    32101 gctgttgcag taataacgcc gttattaaaa ggcctgccat ctggcaggct ttttttatcg
    32161 ctaaataata cagcaacctt taataatctt ctgctgaata aagattatct catatattaa
    32221 ttttatgaga tttttttaga attatatcaa ggagaagaaa caaacttatt aagctagaat
    32281 agccacgggt gcttgagact gtttgtctca ggtattcacc gaaaggcaga cagagaaaag
    32341 ccccacctga ctataaatca aa
//
LOCUS       DAPGRN010000050        32201 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPGRN010000050 DAPGRN010000000
VERSION     DAPGRN010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.050"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(95..1855)
                     /locus_tag="RHW33_002433"
     CDS             complement(95..1855)
                     /locus_tag="RHW33_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HDV1645928.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1985..2677
                     /locus_tag="RHW33_002434"
     CDS             1985..2677
                     /locus_tag="RHW33_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV1645929.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2876..3964
                     /gene="serC"
                     /locus_tag="RHW33_002435"
     CDS             2876..3964
                     /gene="serC"
                     /locus_tag="RHW33_002435"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV1645930.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NVACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4035..5318
                     /gene="aroA"
                     /locus_tag="RHW33_002436"
     CDS             4035..5318
                     /gene="aroA"
                     /locus_tag="RHW33_002436"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV1645931.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALAALGVSYTLSADRTRCEIIGNGGPLHAEGTLELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEVENQ
                     HYQQFVVKGGQPYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5487..6251
                     /gene="ycaL"
                     /locus_tag="RHW33_002437"
     CDS             5487..6251
                     /gene="ycaL"
                     /locus_tag="RHW33_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV1645932.1"
                     /translation="MKNTKLLLAIVTSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6424..7107
                     /gene="cmk"
                     /locus_tag="RHW33_002438"
     CDS             6424..7107
                     /gene="cmk"
                     /locus_tag="RHW33_002438"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV1645933.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7218..8891
                     /gene="rpsA"
                     /locus_tag="RHW33_002439"
     CDS             7218..8891
                     /gene="rpsA"
                     /locus_tag="RHW33_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV1645934.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9051..9335
                     /gene="ihfB"
                     /locus_tag="RHW33_002440"
     CDS             9051..9335
                     /gene="ihfB"
                     /locus_tag="RHW33_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV1645935.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9542..11806
                     /locus_tag="RHW33_002441"
     CDS             9542..11806
                     /locus_tag="RHW33_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV1645936.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLASLTVFACVLVFIPF
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPVPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLRASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLVAGLIRSGQILLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHIWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     VDDGTNSILLTGDIEAPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVDFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11843..13591
                     /gene="msbA"
                     /locus_tag="RHW33_002442"
     CDS             11843..13591
                     /gene="msbA"
                     /locus_tag="RHW33_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV1645937.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13588..14574
                     /gene="lpxK"
                     /locus_tag="RHW33_002443"
     CDS             13588..14574
                     /gene="lpxK"
                     /locus_tag="RHW33_002443"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV1645938.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRTWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14611..15843
                     /locus_tag="RHW33_002444"
     CDS             14611..15843
                     /locus_tag="RHW33_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV1645939.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECNTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGHC
                     PQGLFTDCRTGWEIDPVA"
     gene            15895..16077
                     /gene="ycaR"
                     /locus_tag="RHW33_002445"
     CDS             15895..16077
                     /gene="ycaR"
                     /locus_tag="RHW33_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV1645940.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16074..16820
                     /gene="kdsB"
                     /locus_tag="RHW33_002446"
     CDS             16074..16820
                     /gene="kdsB"
                     /locus_tag="RHW33_002446"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV1645941.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKSAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKGLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            16974..17867
                     /locus_tag="RHW33_002447"
     CDS             16974..17867
                     /locus_tag="RHW33_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV1645942.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17844..18623)
                     /gene="elyC"
                     /locus_tag="RHW33_002448"
     CDS             complement(17844..18623)
                     /gene="elyC"
                     /locus_tag="RHW33_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV1645943.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIISAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18759..19544
                     /gene="cmoM"
                     /locus_tag="RHW33_002449"
     CDS             18759..19544
                     /gene="cmoM"
                     /locus_tag="RHW33_002449"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV1645944.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19541..20863
                     /gene="mukF"
                     /locus_tag="RHW33_002450"
     CDS             19541..20863
                     /gene="mukF"
                     /locus_tag="RHW33_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV1645945.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20844..21548
                     /gene="mukE"
                     /locus_tag="RHW33_002451"
     CDS             20844..21548
                     /gene="mukE"
                     /locus_tag="RHW33_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV1645946.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21548..26008
                     /gene="mukB"
                     /locus_tag="RHW33_002452"
     CDS             21548..26008
                     /gene="mukB"
                     /locus_tag="RHW33_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV1645947.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQACAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLTLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHMAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNNDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26269..28116
                     /gene="ldtD"
                     /locus_tag="RHW33_002453"
     CDS             26269..28116
                     /gene="ldtD"
                     /locus_tag="RHW33_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1645948.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGILPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28297..28845
                     /locus_tag="RHW33_002454"
     CDS             28297..28845
                     /locus_tag="RHW33_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV1645949.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28872..29519
                     /gene="gloC"
                     /locus_tag="RHW33_002455"
     CDS             28872..29519
                     /gene="gloC"
                     /locus_tag="RHW33_002455"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV1645950.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29570..30760)
                     /gene="aspC"
                     /locus_tag="RHW33_002456"
     CDS             complement(29570..30760)
                     /gene="aspC"
                     /locus_tag="RHW33_002456"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV1645951.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30945..32018)
                     /gene="ompF"
                     /locus_tag="RHW33_002457"
     CDS             complement(30945..32018)
                     /gene="ompF"
                     /locus_tag="RHW33_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV1645952.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKDNGVNSYGGNGDKTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQKGNK
                     TRLAFAGLKFADAGSIDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERAGIPERSNGDGVGGSISYEFEGFGIVGAYGAAD
                     RTDAQEAEFRGQGKKAEQWATGLKYDANNIYLAANYGETRNATPIEGGFANKTQDVLL
                     VAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVDYIINQ
                     IDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7834 a         7841 c         8672 g         7854 t
ORIGIN      
        1 taatgcaaat atatgtaaag cggggcatta aaaaaacgcc ccgtaatata actcagacga
       61 atcattaagc ctacattgcg taggctattt gattttattt tgcccaaaat gctgctttgg
      121 cgcgctgcag cttttcgtag gccttcaaca gcgactgatg tgcagcaaac gcgtgcagat
      181 cgctatctac tggttgcaag ccataaaacg ccgcttcgcc gctcattgcc gcactggcgg
      241 cttccacggc atctgcgccg tacatgcgaa caaacgcatt cagatattgc agcggctggc
      301 gatcttcttc ctgtgccagt aataacaacg tttgcaggca gcgataatag ttggcgcgtt
      361 ccgggctgaa taccgatgag ttaaactcca tcgtccattc ggtccagacc agagcctgtt
      421 ccagatcgcc accagccagc gccagcatgg cttttaattc accgatacgc agggtgtacc
      481 aaccgttatc cgacccggtc gccagaccca acagctcacg cacgcgggta aagtcatcaa
      541 aaccttcttc atccagttgc tcgatgaggt tcaggtaatc ttctttttcc cactcgctgc
      601 ctggtagcga aagaatcgtt tcacgtaaat ggctgcccat actgttattc gcgagccaca
      661 gatcttcagc cggataaata tcggacatgc caggcacgat aatacggcaa gcataaacgc
      721 ccagatgctc gtaatcggca atataaactt ctttatcttc tttgttgaag atagccatca
      781 gcgtagcaaa ctcttcttcc gtggtgccgg agaaattcca gtccacaaac ggataatcgg
      841 catcctgctt gaacaggtcc caggagatta aaccgctgga atcgataaag tgcgtttcga
      901 ggttggtatg ttcagcgact tcttcatcat cgaaggttgg cggagtaaac acatccaaat
      961 ctttcaggcc acgaccttgc agcagctcgg tcactgtacg ttccagtgct acgccaaaat
     1021 caggatgcgc accgaaagag gcaaagcagg tgccgttagc aggattaaac agtaccacgc
     1081 aaatcaccgg atactggccg ccaagggaac catcatatgc gaagatcggg aaaccttctg
     1141 cttccagtgt ttcgatcgct tcaaccaccg ctgggtaacg cgccagcacg tctgccggga
     1201 tttccggcaa gctgatgctt tcagcaataa tgcggttttt cacgtagcgt tcgaaaactt
     1261 cggacaaccc ctgaacgcgt gcttcgttac gggtattgcc agcagacata ccgttagaaa
     1321 cgtacaggtt accaatgata ttcatcggaa tataaacggt ctgattgtcg gactggcgcg
     1381 taaacggcag accgcaaata ccacgatctt cgttaccgga ttgtaggtca atcagcatac
     1441 tgccggtcaa ttcattctcc ggatcgtaaa atgcgcgcag acggtcatcg agcagccctt
     1501 ctggcacatc gtcattttcg gtcagtggga accatttttc gttgggataa tgcacaaatg
     1561 gaccgttggc gatggtttcg cccagccaga aatcagcaaa aaagtagttg gttgagagac
     1621 gctcgaaata ttcaccgagt gcagaagcca gcgccgcttt cttggttgcg cctttaccgt
     1681 tggtaaaaca cagtgcgcac tctttgtcgc gaatatgtac agaccagacg ttaggcacgg
     1741 gattcagcca ggaggcctct tcaatctgaa agccgaggtc tgaaagtttt tgctggaagc
     1801 gagcgatgga atcttccagt gcggcatctt tgccggggat aaatgtttgc gtcatgaaaa
     1861 tcactttagt cgtacggaaa gcgcgcaata atacgggttt tatctcaaag gcgctatcac
     1921 cgccgccatg ccgatgaact gttgactatg cttttagcgg ataaaccacg ttaagagcat
     1981 aaaaatgaag gcgttcgatc tccaccgtat ggcatttgat aaagtgcctt ttgatttcct
     2041 tggcgaagtt gcactacgta gtctgtatac ctttgtactg gtttttttgt tcctcaaaat
     2101 gaccggaaga cgcggagtgc ggcagatgtc gctgtttgaa gtgttaatca ttctgacgct
     2161 ggggtcagcg gcgggagatg tggcgtttta tgatgatgtg ccgatggtcc cggtacttat
     2221 cgtctttatc actctggcgt tgttataccg cctggtaatg tggttgatgg cacacagtga
     2281 aaaactggaa gatcttctgg aaggtaagcc ggttgtcatt attgaagatg gcgagctggc
     2341 ctggtcgaaa ctcaataact ccaacatgac ggaatttgag ttctttatgg agctacgatt
     2401 gcgcggtgta gagcagttgg ggcaagtacg tctggcgatt ctcgaaacca acgggcaaat
     2461 cagtgtctat ttctttgaag atgacaaggt gaaaccgggt ttacttattt tacccagtga
     2521 ttgtacgcag cgttacaaag tggtgccgga gtcggcggac tatgcctgta ttcgttgtag
     2581 tgaaatcatt catatgaaag cgggggaaaa acaattatgt ccgcgctgtg caaatccaga
     2641 atggacgaag gcaagtcggg caaaacgggt gacctgacag taaaaacatc ggctttttgc
     2701 taataatccg agagattctt ttgtgtgatg caagccacat ttttgccctc aacggtttta
     2761 ctcattgcga tgtgtgtcac tgaatgataa aaccgatagc cacaggaata atgtattacc
     2821 tgtggtcgca atcgattgac cgcgggttaa tagcaacgca acgtggtgag gggaaatggc
     2881 tcaaatcttc aattttagtt ctggtccggc aatgctaccg gcagaggtgc ttaaacaggc
     2941 tcaacaggaa ctgcgcgact ggaacggtct tggtacgtcg gtgatggaag tgagtcaccg
     3001 tggcaaagag ttcattcagg ttgcagagga agccgagaag gattttcgcg atcttcttaa
     3061 tgtcccctcc aactacaagg tattattctg ccatggcggt ggtcgcggtc agtttgctgc
     3121 ggtaccgctg aatattctcg gtgataaaac caccgcagat tatgttgatg ccggttactg
     3181 ggcggcaagt gccattaaag aagcgaaaaa atactgcacg cctaatgtct ttgacgccaa
     3241 agtgactgtt gatggtctgc gcgcggttaa gccaatgcgt gaatggcaac tctctgataa
     3301 tgctgcttat atgcattact gcccgaatga aaccatcgac ggtatcgcca tcgacgaaac
     3361 gccagacttc ggcaaagatg tggtggtcgc cgccgacttc tcttcaacca ttctttcccg
     3421 tccgattgac gtcagccgtt atggtgtaat ttacgctggc gcgcagaaaa atatcggccc
     3481 ggcaggcctg acaatcgtca tcgttcgtga agatttgctg ggcaaagcga atgtcgcgtg
     3541 tccgtcgatt ctggattatt ccatcctcaa cgataatggt tccatgttta acacgccgcc
     3601 gacatttgcc tggtatctat ctggtctggt ctttaaatgg ctgaaagcga acggcggtgt
     3661 agctgaaatg gataaaatca atcagcaaaa agcagaactg ctatatgggg tgattgataa
     3721 cagcgatttc taccgcaatg acgtggcgaa agctaaccgt tcgcggatga acgtaccgtt
     3781 ccagttggca gacagtgcgc ttgacaaatt gttccttgaa gagtcttttg ctgctggcct
     3841 tcatgcactg aaaggtcacc gtgtggtcgg cggaatgcgc gcgtctattt ataacgccat
     3901 gccgctggaa ggcgttaaag cgctgacaga cttcatggtt gagttcgaac gccgtcacgg
     3961 ctaatgccga aattttgctt aatccccaca gccagcctgt ggggttttta tttctgttgt
     4021 agagagttga gttcatggaa tccctgacgt tacaacccat cgctcgtgtc gatggcacta
     4081 ttaatctgcc cggttccaag agcgtttcta accgcgcttt attgctggcg gcattagcac
     4141 acggcaaaac agtattaacc aatctgctgg atagcgatga cgtgcgccat atgctgaatg
     4201 cattagcagc gttaggggta agctatacgc tttcagccga tcgtacgcgt tgcgaaatta
     4261 tcggtaacgg cggtccatta cacgcagaag gtaccctgga gttgttcctc ggaaacgccg
     4321 gaacggcaat gcgcccgctg gcggcagctc tttgtctggg tagcaatgat attgtgctga
     4381 ccggtgagcc gcgtatgaaa gagcgcccga ttggtcatct ggtggatgcg ctgcgcctgg
     4441 gcggggcgaa gatcacttac ctggaacaag aaaattatcc gccattgcgt ttacagggcg
     4501 gctttacagg tggcaatgtt gacgttgatg gttccgtttc cagtcaattc ctcaccgcac
     4561 tattaatgac tgcgccgctt gcgccggaag atacagttat tcgtattaaa ggcgatctgg
     4621 tttctaaacc ttatatcgac atcacactca atctgatgaa gacgtttggt gttgaagttg
     4681 aaaatcagca ctatcaacaa tttgtggtga aaggtggtca accttatcag tcaccgggta
     4741 cttatttggt cgaaggcgat gcatcttcag cttcttactt cctggcagca gcagcaatca
     4801 aaggcggaac tgtaaaagtg accggcattg gacgcaacag tatgcagggt gatattcgct
     4861 ttgctgatgt gctggaaaaa atgggcgcga ccatttgctg gggcgatgat tatatttcct
     4921 gtacgcgtgg tgaactgaac gctattgata tggatatgaa ccatattcct gatgcggcga
     4981 tgaccattgc cacggcggcg ttatttgcaa aaggcaccac cacgctgcgc aatatctata
     5041 actggcgtgt taaagaaacc gatcgcctgt ttgcgatggc aacagaactg cgtaaagtcg
     5101 gtgcggaagt agaagagggg cacgattaca ttcgtatcac tccaccggaa aaactgaact
     5161 ttgccgagat cgcgacatac aatgatcacc ggatggcgat gtgtttctcg ctggtggcgt
     5221 tgtcagatac accagtgacg attcttgatc ccaaatgtac ggccaaaaca tttccggatt
     5281 atttcgagca gctggcgcgg attagccagg cagcctgaat gaataacgtc caataaatag
     5341 ccaaaacttt ctttatcaaa acgtcggcac attgccggcg ttttttttcg gaccttgtga
     5401 gtcattttga ttaatggtag cgtcagttgt caatgtaagt tgttgataca taatatttat
     5461 atatgattaa tcaacggatg attcacatga agaatactaa attactgctg gcgattgtga
     5521 cctctgcagc attactgacg gggtgtcaaa atacccacgg tattgatacc aatatggcta
     5581 tcagctccgg tttaaatgcc tataaagcag caacattaag cgatgccgat gcaaaagcga
     5641 ttgccaatca gggctgtgcc gaaatggaca gcggcaatca agtcgcaagt aaatccagca
     5701 agtacggtaa acgtctggca aaaatcgcca aagcattggg taacaatatt aatggcacgc
     5761 cggtcaacta taaggtttat atgaccagcg acgtcaacgc atgggcgatg gcgaacggct
     5821 gtgttcgtgt ttacagtggc ctgatggaca tgatgaacga taacgaaatt gaaggcgttc
     5881 tgggccatga actgggccac gtcgcgttgg gtcactcgct ggctgaaatg aaagcttctt
     5941 atgcgatcgt tgccgcacgc gatgccattt cagctaccag cggtgtggct tcccagcttt
     6001 cccgctcaca attgggtgat atcgcagaag gcgctatcaa tgcgaaatac tcccgcgata
     6061 aagagtccga agcagatgat ttctcctttg atctgttgaa gaaacgtggc atcagcaccc
     6121 aggggctggt tggcagcttc gaaaaactgg ctagcctgga tggcggtcgc acccagtcca
     6181 tgtttgactc tcacccacca tcaacagagc gtgcacaaca catccgtgat cgtatcgcct
     6241 ctggtaagta aatcattgtc atctttcggg ctggtcttct gccagcccgc tataattgcg
     6301 caataaatcc ccatctgaat acatacaaaa ctggtttttg cacacaacgt taacgatttg
     6361 tggcgtcggc gcgtataatg cgcgcggtta tgttaacggt acgcctgttt taaggagata
     6421 aagatgacgg caattgcccc ggttattacc attgatggcc caagcggtgc agggaaaggc
     6481 accttgtgta aggctatggc ggaagcgttg caatggcatc tgctggactc gggtgcaatt
     6541 tatcgcgtac tggcattggc ggcattacat caccatgttg atgttgcgtc ggaagatgcg
     6601 ctggtaccgc tggcatccca tctggatgta cgttttgtgt caaccaatgg caatctggaa
     6661 gtgatcctcg aaggggaaga tgtcagcggc gaaattcgta ctcaggaagt ggcgaatgca
     6721 gcttcacaag tcgcggcatt cccacgcgtt cgtgaagcat tattgcgtcg ccaacgcgcg
     6781 tttcgcgaat taccaggcct gattgccgat ggccgcgaca tgggaacggt ggtattccct
     6841 gatgcgccag tgaaaatttt ccttgacgcc tcctcggaag aacgtgcgca tcgccgcatg
     6901 ctacagttgc aggagaaggg ctttagtgtt aactttgagc gccttttggc cgagatcaaa
     6961 gaacgcgacg accgcgatcg taaccgagcg gtagcgccac tggttccggc agccgatgct
     7021 ttagtgttgg attccaccac cttaagcatt gagcaagtga ttgaaaaagc gctacaatac
     7081 gcgcgccaga aattggctct cgcataagcg accgaatttg cagtaccccc gttgcaatgg
     7141 aatgacagcg ggtatgttaa acaaccccat ccggcatgga gccaggtgga cgttaaatat
     7201 aaacctgaag attaaacatg actgaatctt ttgctcaact ctttgaagag tccttaaaag
     7261 aaatcgaaac ccgcccgggt tctatcgttc gtggcgttgt tgttgctatc gacaaagatg
     7321 tagtactggt tgacgctggt ctgaaatctg agtccgccat cccggctgag cagttcaaaa
     7381 acgcccaggg cgagctggaa atccaggtag gtgacgaagt tgacgttgct ctggacgcag
     7441 tagaagacgg cttcggtgaa actctgctgt cccgtgagaa agctaaacgt cacgaagcct
     7501 ggatcacgct ggaaaaagct tacgaagatg ctgaaactgt taccggtgtt atcaacggca
     7561 aagttaaggg cggcttcact gttgagctga acggtattcg tgcgttcctg ccaggttctc
     7621 tggtagacgt tcgtccggtg cgtgacactc tgcacctgga aggcaaagag cttgaattca
     7681 aagtaatcaa gctggatcag aagcgcaaca acgttgttgt ttctcgtcgt gccgttatcg
     7741 aatccgaaaa cagcgcagag cgcgatcagc tgctggaaaa cctgcaggaa ggcatggaag
     7801 ttaaaggtat cgttaagaac ctcactgact acggtgcatt cgttgatctg ggcggcgttg
     7861 acggcctgct gcacatcact gacatggcct ggaaacgcgt taagcatccg agcgaaatcg
     7921 tcaacgtggg cgacgaaatc actgttaaag tgctgaagtt cgaccgcgaa cgtacccgtg
     7981 tatccctggg cctgaaacag ctgggcgaag atccgtgggt agctatcgct aaacgttatc
     8041 cggaaggtac caaactgact ggtcgcgtga ccaacctgac cgactacggc tgcttcgttg
     8101 aaatcgaaga aggcgttgaa ggcctggtac acgtttccga aatggattgg accaacaaaa
     8161 acatccaccc gtccaaagtt gttaacgttg gcgatgtagt ggaagttatg gttctggata
     8221 tcgacgaaga acgtcgtcgt atctccctgg gtctgaaaca gtgcaaagct aacccgtggc
     8281 agcagttcgc ggaaacccac aataagggcg accgtgttga aggtaaaatc aagtctatca
     8341 ctgacttcgg tatcttcatc ggcctggacg gcggcatcga cggcctggtt cacctgtctg
     8401 acatctcctg gaacgttgca ggcgaagaag cagttcgtga atacaaaaaa ggcgacgaaa
     8461 tcgctgcagt tgttctgcag gttgacgcag aacgtgaacg tatctccctg ggcgttaaac
     8521 agctcgcaga agatccgttc aacaactggg ttgctctgaa caagaaaggc gctatcgtaa
     8581 ccggtaaagt aactgcagtt gacgctaaag gcgcaaccgt agaactggct gacggcgttg
     8641 aaggttacct gcgtgcttct gaagcatccc gtgaccgcgt tgaagacgct accctggttc
     8701 tgagcgttgg cgacgaagtt gaagctaaat tcaccggcgt tgatcgtaaa aaccgcgcaa
     8761 tcagcctgtc tgttcgtgcg aaagacgaag ctgacgagaa agatgcaatc gcaactgtta
     8821 acaaacagga agatgcaaac ttctccaaca acgcaatggc tgaagctttc aaagcagcta
     8881 aaggcgagta attctctgac tcttcgggat ttttattccg aagattgttg agtttacttg
     8941 acagattgca ggtttcgtcc tgtaatcaag cactaagggc ggctacggcc gcccttaatc
     9001 aatgcagcaa cagcagccgc ttaatttgcc tttaaggaac cggaggaatc atgaccaagt
     9061 cagaattgat agaaagactt gccacccagc aatcgcacat tcccgccaag acggttgaag
     9121 atgcagtaaa agagatgctg gagcatatgg cctcgactct tgcgcagggc gagcgtattg
     9181 aaatccgcgg tttcggcagt ttctctttgc actaccgcgc accacgtacc ggacgtaatc
     9241 cgaagactgg cgataaagta gaactggaag gaaaatacgt tcctcacttt aaacctggta
     9301 aagaactgcg cgatcgcgcc aatatttacg gttaagtttt tactcaaact tgaactcgag
     9361 aaaagcacct gtcgggtgct tttttcattt ctctaatctg gaactggaag ctgcctcgca
     9421 gagttttgag cagttttcac cctctcgtta aattcttctg aatgtgcctc ggggaacgca
     9481 aaattcccac cagacaaccg ctcaacaaag ttgcacactt tccataaaca gggaggggtg
     9541 catgaaaata acgacagtcg gcgtatgcat aatttgcgga atttttccgt tgctgatttt
     9601 gccccaattg cctgggacat taacccttgc gtctctgacc gtcttcgcct gcgtactggt
     9661 atttatccct tttaaaaccg tccgttatat cgcgctgacg ttgctgtttt tcgtttgggg
     9721 catattagca gcaaagcaaa ttttgtgggc aggagaaacc ttaactggcg cgacgcagga
     9781 tgcaattgtt gagatcactg ctactgacgg tatgaccact cattacggtc aaattactca
     9841 tctacaaggt cgacgtatat tccctgtgcc aggcctcgtg ctgtatggcg aatatcttcc
     9901 gcaagcggtt tgtgctggac aacaatggtc aatgaaactc aaagttcgtg ccgttcatgg
     9961 tcaacttaat gatggcggct ttgatagcca gcgttatgcc attgcccagc atcagccgct
    10021 caccggccgt tttctgcggg caagtgtcat tgaaccgaat tgtagcctgc gtgcacagta
    10081 tctggcgtca ctacaaacaa cgctgcaacc ctatatgtgg aatgcggtta ttcttggttt
    10141 aggtatgggg gaacggttat ccgtccctaa agaaatcaaa aatatcatgc gtgatactgg
    10201 aacggcgcat ttaatggcga tatcgggatt gcacatcgct tttgcggcgt tgctggttgc
    10261 cggactcatt cgcagtggac aaattttgct gcctgggcgc tggatccact ggcaaatgcc
    10321 attaattggc ggaatctgct gtgctgcttt ttatgcctgg ctgacgggaa tgcaacctcc
    10381 tgcattgcgt accgtggtgg cgcttgctac gtggggaatg cttaagttaa gtgggcgaca
    10441 gtggagtggt tgggatgtat ggatatgttg tctggcggca attttgctga tggatcctgt
    10501 tgccattctc tcgcaaagtt tatggctctc tgccgctgcg gtcgcggcac tgattttttg
    10561 gtaccagtgg tttccctgtc ctgagtggca actgccgccg gtattgcgtg cagttgtttc
    10621 cctcatccat ctgcaactgg gaatcacact tctgcttatg cccgtgcaaa tcgtcatatt
    10681 tcatggcatt agtctgacct cgtttattgc aaatctatta gcaattccct tggtgacatt
    10741 tatcacggtt ccgttgatcc tcgccgcgat ggttgtgcat ttaagcgggc cgttaatcct
    10801 ggagcaaggg ttatggtttc ttgccgaccg gtctttggct ttacttttct gggggttaaa
    10861 gagtttgcca gaagggtgga tcaacattgc tgaacgttgg caatggctat cattttctcc
    10921 atggttctta ctggtggtat ggcgattaaa cgcctggcga acgttgccag caatgtgtgt
    10981 ggctgtaggc ttgctgatgt gctggccgct gtggcaaaaa cctcgacctg acgagtggca
    11041 agtgtacatg cttgatgtcg ggcaagggct ggcaatggtg atagccagaa acggcaaagc
    11101 gattctctat gacacgggac tggcctggcc cgaaggggat agtgggcaac aactgattat
    11161 cccctggctc cactggcata atcttgaacc ggaaggcgtt attctgagcc atgaacatct
    11221 ggatcaccgg ggagggctgg attcaatatt gcatatatgg ccgatgttat ggatcagaag
    11281 tccgttaaac tgggaacatc atcagccctg tgtgcgtggc gaagcgtggc aatggcaagg
    11341 attgcgtttc agcgcgcact ggcctttaca aggtagcaac gataaaggaa ataaccattc
    11401 ctgtgtggtt aaggttgatg acgggacgaa tagcattctt ctaaccggtg atattgaagc
    11461 cccagctgaa caaaagatgc taagccgtta ctggcagcaa gtgcaggcaa cattgcttca
    11521 ggtacctcac catggcagta atacctcatc atcgttgcca ttaattcagc gagtgaatgg
    11581 aaaagtggca ctcgcatcgg catcgcgcta taacgcatgg cgactgccct ctaacaaagt
    11641 taagcatcgc tatcaacaac agggttatac gtggcttgat actcctcatc aggggcaagt
    11701 aacggtcgat ttttcagcgc aaggctggcg gattagcagc ctcagggagc aaattttacc
    11761 tcgttggtat catcagtggt ttggcgtgcc agtggataac gggtagaata tgcggctatt
    11821 tcaacaaatg ctggtttttt gaatgcataa cgacaaagat ctctctacgt ggcagacatt
    11881 ccgccgactg tggccaacca ttgcgccttt taaagcgggt ctgatcgtgg cgggcgtagc
    11941 gttaatcctc aacgcagcca gcgatacctt catgttatcg ctccttaagc cacttcttga
    12001 tgatggcttt ggtaaaacag atcgctccgt gctggtgtgg atgccgctgg tggtgatcgg
    12061 gctgatgatt ttgcgtggta tcaccagcta tgtctccagc tactgtatct cctgggtatc
    12121 aggaaaggtg gtaatgacca tgcgtcgccg cctgtttggt cacatgatgg gaatgccggt
    12181 ttcattcttt gacaaacagt caacgggtac gctgttgtca cgtatcacct acgattccga
    12241 acaggttgct tcttcctctt ctggcgcact gattactgtt gtgcgtgaag gtgcgtcgat
    12301 catcggcctg ttcatcatga tgttctatta cagttggcaa ttgtcgatca ttttgattgt
    12361 gctggcaccg attgtttcga ttgcgattcg cgtagtatcg aagcgttttc gcaacatcag
    12421 taaaaacatg cagaacacca tggggcaggt gaccaccagc gctgaacaaa tgctgaaagg
    12481 ccataaagaa gtattgattt tcggtggtca ggaagtggaa acgaaacgct tcgataaagt
    12541 cagcaaccga atgcgtcttc aggggatgaa aatggtttca gcctcttcca tctctgatcc
    12601 gatcattcag ctgatcgcct ctttggcgct ggcgtttgtt ctgtatgcgg cgagcttccc
    12661 aagtgtcatg gatagcctga ctgccggtac gattaccgtt gttttctctt caatgattgc
    12721 actgatgcgt ccgctgaaat cgctgactaa cgttaacgcc cagttccagc gcggtatggc
    12781 ggcttgtcag acgctgttta ccattctgga cagtgagcag gagaaagacg aaggtaagcg
    12841 cgtgatcgag cgtgcgactg gcgacgtgga attccgcaac gtcaccttta cttatccggg
    12901 acgtgacgta cccgcattgc gtaacatcaa cctgaaaatt ccggcaggga agacggttgc
    12961 tctggttgga cgctctggtt caggtaaatc aaccatcgcc agcctgatca cgcgttttta
    13021 cgatattgat gaaggcgaaa tcctgatgga tggtcacgat ctgcgcgagt ataccctggc
    13081 gtcgttacgt aaccaggttg ctctggtgtc gcagaatgtc catctgttta acgatacggt
    13141 tgctaacaac attgcttacg cacggactga acagtacagc cgtgagcaaa ttgaagaagc
    13201 ggcgcgtatg gcctacgcca tggacttcat caataagatg gataacggtc tcgatacagt
    13261 gattggtgaa aacggcgtgc tgctctctgg cggtcagcgt cagcgtattg ctatcgctcg
    13321 agccttgctg cgtgatagcc cgattctgat tctggatgaa gctacctcgg ctctggatac
    13381 cgaatccgaa cgtgcgattc aggcggcact ggatgagttg cagaaaaacc gtacctctct
    13441 ggtgattgcc caccgcttgt ctaccattga aaaggcagac gaaatcgtgg tcgtcgagga
    13501 tggtgtcatt gtggaacgcg gtacgcataa cgatttgctt gagcaccgcg gcgtttacgc
    13561 gcaacttcac aaaatgcagt ttggccaatg atcgaaaaaa tctggtctgg tgaatcccct
    13621 ttgtggcggc tattgctgcc actctcctgg ttgtatggcc tggtgagtgg cgcgatccgt
    13681 ctttgctata aactaaaact gaagcgcacc tggcgtgccc ccgtaccggt tgtcgtggtt
    13741 ggtaatctca ccgcaggcgg caacggaaaa accccggtcg ttgtctggct ggtggaacag
    13801 ttgcaacagc gcggtattcg cgtgggggtc gtatcgcggg gatatggtgg taaggctgaa
    13861 tcttatccgc tgttattgtc ggcagatacc accacagcac aggcgggtga tgaacctgtg
    13921 ttgatttatc aacgcactga tgcgcctgtt gcggtttctc ccgttcgttc tgatgcggta
    13981 aaagccattc tggcgcaaca ccctgatgtg cagatcatcg taaccgacga cggtttacag
    14041 cattaccgtc tggcgcgtga tgtggaaatt gtcgttattg atggtgtgcg tcgctttggc
    14101 aatggctggt ggttgccggc ggggccaatg cgtgagcgag cggggcgctt aaagtcagtt
    14161 gatgcggtaa tcgtcaacgg cggtgtcccc cgcagcggtg aaatccccat gcatctgctg
    14221 ccgggtcagg cggtgaattt acgtaccggt acgcgttgtg acgttgctca gcttgaacat
    14281 gtggtggcga tggcagggat tgggcatccg ccgcgctttt ttgccacgct gaagatgtgc
    14341 ggcgtacaac cggaaaaatg tgtaccgctg gccgatcatc agtctttgaa ccatgcggat
    14401 gtcagcgcgt tggtaagcac cgggcaaacg ctggtaatga ctgaaaaaga tgcggtgaaa
    14461 tgccgggcct ttgcagaaga aaattggtgg tatttgcccg ttgacgcaca gctttcaggt
    14521 gatgaaccag cgaaactgct tgcgcaacta acctcgctgg cttctggcaa ctagttacgc
    14581 cgcggcagcg ttcgattgat ggagtcatga atgtcgctgc cgcacctctc ccttgctgat
    14641 gcgcgtaatc ttcaccttgc cgcgcaaggc ctgttaaaca aaccccgccg tcgagcgtcg
    14701 ttggaggata ttccggcaac gatctcccgc atgtccttgc tgcaaatcga taccatcaat
    14761 attgttgccc gtagcccata tctggtgctt ttcagtcgtc tgggaaatta tcctgcccag
    14821 tggctggatg agtctctggc gcgtggcgaa ttaatggaat actgggcgca tgaagcctgc
    14881 tttatgccac gcagcgactt tcgtcttatt cgccaccgca tgctggcacc tgaaaaaatg
    14941 ggctggaaat acaaagacgc ctggatgcag gaacatgagg cggaaattgc acagttaatt
    15001 cagcatattc atgataaggg gccggtacgt tcagccgatt ttgagcatcc tcgtaaaggt
    15061 gcaagcggct ggtgggaatg gaagccgcat aaacggcatc tggaaggttt atttactgcc
    15121 ggaaaggtga tggtgattga acggcgcaac ttccagcgcg tttatgattt aacccaccgt
    15181 gtcatgcctg actgggatga tgagcgcgat ctcgtttcgc aaacagaagc agaaatcatc
    15241 atgctggata acagtgcgcg tagcctggga atattccgcg aacagtggct ggcagattac
    15301 tatcggctga aacgtccggc actggcggcg tggcgtgaag cgagggctga acagcagcaa
    15361 atcattgctg tgcatgttga aaaattgggc aatctttggc tgcatgctga tttgctgccg
    15421 ctactcgagc gagcgctggc cggaaagctc actgcaacgc acagcgcggt actttcgcct
    15481 tttgatcctg ttgtctggga tcgcaaacgc gcagagcagc tttttgattt tagctaccgg
    15541 ctggagtgca atacccctgc gccgaaacgt cagtatggct attttgttct gccattatta
    15601 catcgtgggc aattagttgg gcgaatggat gccaaaatgc atcgccagac aggcatcctt
    15661 gaagttatct ctctgtggtt acaggaaggc attaaaccaa cgacaatgct gcaaaaaggg
    15721 ttacgtcagg cgattactga tttcgcgagc tggcagcagg caacgcgggt gacattagga
    15781 cactgcccgc aaggtctctt tacggattgc cgcaccggct gggaaataga ccccgtcgca
    15841 taaatgatta tgataagatt tatgcattca taacccgatc catctggagg aactatggat
    15901 catcgtctgc ttgaaatcat tgcctgcccg gtttgcaacg gaaaactttg gtataaccag
    15961 gaaaaacaag aacttatttg caaactggac aacctggctt tcccgctgcg cgatggcatt
    16021 ccagtgttgc tggaaacgga agcccgcgtg ctgactgctg atgagagtaa atcatgagtt
    16081 ttgtggtcat tattcccgcg cgctacgcgt ccacgcgtct gcccggtaaa ccattggttg
    16141 atattaacgg caaacccatg attgttcatg ttcttgaacg cgcgcgtgaa tcaggtgccg
    16201 agcgcatcat cgtggcaacc gatcatgagg atgtcgcccg cgccgttgaa gccgctggcg
    16261 gtgaagtatg tatgacgcgc gccgatcacc agtcagggac agaacgcttg gcggaagttg
    16321 tcgaaaaatc cgcgttcagc gacgacacgg tgatcgttaa tgtgcagggt gatgaaccga
    16381 tgatccctgc gacaatcatt cgtcaggttg ctgataacct cgctcagcgt caggtgggta
    16441 tggcgactct ggcggtgcca atccacaatg cggaagaagc gtttaacccg aatgcggtga
    16501 aagtggttct cgacgctgaa gggtatgcac tgtacttctc acgcgctacc attccgtggg
    16561 atcgtgatcg ttttgcaaaa ggccttgaaa ccgttggcga taacttcctg cgtcaccttg
    16621 gtatttatgg ctaccgtgca ggctttatcc gtcgttacgt cacctggcag ccaagcccgt
    16681 tagaacacat cgaaatgtta gagcagcttc gtgttctgtg gtacggcgaa aaaatccatg
    16741 ttgctgtcgc gcatgaagtt cctggcacag gtgtggatac ccctgaagat ctcgagcgcg
    16801 ttcgcgctga aatgcgctaa tttcacttca cgacactcca gccaattttg ggaggagtgt
    16861 cgtaccgtta cgattttcct caatttttct tttcaacaat tgatctcatt caggtgacat
    16921 cttttatatt ggcgctcatt atgaaagcag tagcttttat gagggtaatc tgaatggaac
    16981 agctgcgtgc cgaattaagc catttactgg gcgaaaaact cagtcgtatt gagtgcgtca
    17041 atgaaaaagc ggatacggcg ttgtgggctt tgtatgacag ccagggaaac ccaatgccgt
    17101 taatggcaag aagctttagt acgcccggaa aagcccgaca actggcatgg aaaaccacca
    17161 tgctggcaag aagtgggact gtccgtatgc cgactattta tggtgtgatg acgcatgaag
    17221 aacaccccgg ccctgatgtc ctgctactgg agcggatgcg tggtgtttcg gtggaggcac
    17281 cagcccgaac accagaacgc tgggaacagc tcaaagacca aatcgttgaa gccttactgg
    17341 cctggcaccg tcaggacagt cgcggttgcg tcggcgcggt cgacaatact caggaaaatt
    17401 tctggccctc atggtaccgg caacatgttg aagtgctatg gaccacgctc aatcagttca
    17461 ataacaccgg tctgacgatg caggataagc ggatcctgtt tcgcactcgc gaatgtctcc
    17521 cggcattatt tgaaggtttt aacgacaatt gtgtgctgat tcacggtaac ttctgtttac
    17581 gcagcatgct gaaagattcg cgcagcgatc agttactggc gatggtcggg ccgggactaa
    17641 tgctttgggc accgcgagaa tacgaactgt tccgactaat ggataattct ctggcggaag
    17701 atttgctctg gagttacctg caacgcgcgc cagtggcgga gtcgttcatc tggcggcgtt
    17761 ggttgtatgt gttatgggat gaagtcgcgc aactggttaa taccggacga tttagtcggc
    17821 gcaacttcga tctggcatca aaatcactct tgccgtggct cgcctgacgg acctttcagc
    17881 cactgccaga tgcggccaag cgtttcatag ccaacacgat cgctatgcat cagccagacc
    17941 ggagaaggga ttgcccgttc ccacggatta agcggcgagt cgatggccaa ctgattcgcc
    18001 ggggctggta gcggatttaa cccttcctgc tgaaaaaaga tcatcgcccg cggcagatgt
    18061 gatgcggagg tcaccagcag gaaaggggcg tcaccaatcg cctgtttcac tgccgcagct
    18121 tcttcttcgg tatcttttgg caaatccagg gtgataattt gctcgcgcgg tacgcccagc
    18181 gattgcgcaa ctctggcacc tacttccgct gtactcaccg tattggtttt tgctacgcct
    18241 cccgtgaaga tcagttttga acccggattt tcgcgccata aacggatacc ctcattcagg
    18301 cgtggcagac tgttattgat taaattagag ctcggtgccc actgcgggtt ccaggtataa
    18361 ccacctccga gcaccacgat atagtccact ttttgcgaat tattccatgt cggataggtg
    18421 ctttcgatgg gacgcagcag gcggtcggct accggttgca aactcaataa caagagtgcc
    18481 agccacccaa tgctaataaa aatcttcccg gttttttgaa aacggctaaa ccacagcagc
    18541 gccaggccag cgctaataat taacaacatc agcggaagtg gcagcaacat attgccaatc
    18601 acttttttca gtgtaaaaag catcctttct ggttcctttt ttaaccatat agcaagggat
    18661 cgccagcgat attacaccag acgggttcat tctcgccgtg gctgtgacaa aatagcgtct
    18721 ttatcagaaa acgtcttagc caaccggtgg agaaagcaat gcaggatcgc aattttgatg
    18781 atattgcgga aaagttttcc cgtaacattt acggcaccac caaagggcag cttcgacagg
    18841 ctattctgtg gcaggatctc gatcgcgtgc tggcggaaat gggtccgcaa aaactgcgtg
    18901 tgctggatgc gggcggtgga gaagggcaga ccgcaatcaa aatggccgag cgcgggcatc
    18961 aggtcatttt atgcgatctt tctgcgcaga tgatcgaccg cgcaaaacag gcggcagaag
    19021 caaaaggtgt gagcgacaac atgcaattta tacattgcgc cgctcaggat gttgcttcgc
    19081 atttggaaac gcccgttgat ctgatattgt tccatgcggt gctcgagtgg gtggctgatc
    19141 cccgcagcgt attgcagacc ctgtggtcag tgttgcgtcc aggcggcgtg ttgtcgttaa
    19201 tgttctacaa tgcgcatggt ttgttgatgc ataacatggt cgccgggaat tttgattacg
    19261 tgcaggcggg aatgccgaaa aagaaaaaac ggacgctttc gccagattat ccacgcgacc
    19321 cggcgcaggt ttatctgtgg ctggaagaag ctggttggca aattatgggt aagacaggcg
    19381 ttcgcgtgtt tcatgattat ctgcgcgaga agcaccagca gcgcgactgc tatgaagcat
    19441 tacttgaatt agaaacgcgt tattgccgtc aggaaccgta tattaccctg gggcgttata
    19501 ttcatgtcac cgcgcgcaaa ccgcagagca aggataaagt atgagtgaat tttcccagac
    19561 agtccccgaa ctggttgcct gggccagaaa aaatgacttc tccatctcgc tgccggtaga
    19621 ccgactctct tttctgctgg cggttgccac gctgaacggc gagcgtctgg atggtgagat
    19681 gagtgaaggc gagctggtgg atgcattccg ccatgtgagt gatgcgtttg agcaaaccag
    19741 cgaaaccatc ggcgtgcgcg ccaataacgc gatcaacgac atggtgcgtc aacgtctgct
    19801 gaaccgcttt accagcgagc aggcagaagg gaacgcaatt taccgtctga cgccgctcgg
    19861 catcggcatt actgactatt acatccgtca gcgcgagttt tctacgctgc gcctttctat
    19921 gcagttgtcg attgtggcgg gtgagctcaa acgcgcagca gatgccgccg aagagggcgg
    19981 tgatgaattt cactggcacc gtaatgtcta tgcgccactg aaatattcgg tagcagaaat
    20041 tttcgacagt atcgacctga cgcaacgtct gatggacgaa cagcagcagc aggtgaagga
    20101 cgatatcgcc cagttgctga acaaagactg gcgggcggcg atttccagct gtgaattgtt
    20161 gctttcggaa acttccggaa cgctgcgtga attgcaggat acgctggaag cggcaggcga
    20221 caaattgcag gctaatctgt tgcgcattca ggatgcgacg atgactcatg acgatctgca
    20281 ttttgtcgat cgtctggtgt tcgatctgca gagcaaactc gaccgtatta tcagttgggg
    20341 ccagcaatcc atcgatttgt ggattggcta cgaccgccac gtacataaat ttattcgtac
    20401 cgcgatcgat atggataaaa accgcgtctt tgctcagcgg ttacgtcagt cggtacaaac
    20461 ctattttgat gagccgtggg cgctaactta tgccaatgcc gatcgtctgc tggatatgcg
    20521 tgacgaagag atggcactgc gcgatgaaga agtgactggg gaacttccgc cagatttgga
    20581 atacgaagag tttaacgaga tccgcgaaca gctggcggcg atcatcgaag aacaacttgc
    20641 cgtgtacaaa accagacaag tgccgctgga tcttggtctg gtggtacgcg aatatctgtc
    20701 acagtatccg cgtgcacgtc actttgacgt tgcgcgcatt gttattgatc aggcggtacg
    20761 tcttggcgta gcgcaagcag atttcaccgg actgccagcg aaatggcagc cgattaatga
    20821 ttacggagcc aaggtacagg cgcatgtcat cgacaaatat tgaacaagtg atgccggtta
    20881 agctggcgca ggcgctggcg aatccgttat ttccggcgct ggacagcgcc ttacgttcag
    20941 gccgccatat tggcctcgac gaactggata atcatgcatt cctgatggat tttcaggaat
    21001 atctggaaga gttttacgcg cgttataacg ttgagcttat tcgtgcaccg gaagggttct
    21061 tctatttacg tccacgttcc accacgctga tccctcgttc cgtcttgtcg gaactggata
    21121 tgatggtcgg gaaaatcctc tgttatctct atctcagccc ggaacggctg gcgaatgagg
    21181 ggattttcac ccagcaggaa ctgtacgacg aactgctcac cctggccgat gaagcgaaac
    21241 tgctgaaact ggtgaacaac cgttcaaccg gttcagacgt tgaccgtcag aagttgcagg
    21301 aaaaagtacg ttcttcgctc aaccgtctgc gtcgtttagg tatggtgtgg tttatgggcc
    21361 acgacagcag caagttccgc attaccgaat cggtgttccg cttcggagcc gatgtgcgtg
    21421 ctggcgacga tccccgtgaa gcacagcgtc gcctgattcg tgatggcgaa gcaatgccga
    21481 ttgaaaatca tctgcaactc aacgatgaaa ccgaagagaa tcagccagat agcggagagg
    21541 aagaataatg attgaacgcg gtaaatttcg ctcactgacg ctgattaact ggaacggctt
    21601 ttttgcccga acttttgacc ttgacgagct ggtcacgacg ctttctggcg gtaacggggc
    21661 gggtaaatcc accaccatgg cggcgttcgt tacggcgctg attcccgacc tgaccctgct
    21721 gcatttccgt aacactacgg aagccggggc caccagcggt tcgcgcgata aaggtctgca
    21781 cggtaagctg aaagcgggtg tctgttattc gatgctcgac accattaact cgcgccacca
    21841 gcgcgtggtg gtcggtgtgc gtctgcaaca ggttgccgga cgcgatcgta aagtggatat
    21901 caaaccattt gccattcagg gactgccgat gtcggtgcag ccgacacagc tggtgaccga
    21961 aactctgaac gaacgccagg cgcgcgtgct gccgcttaac gagctgaaag acaagctcga
    22021 ggcgatggaa ggcgtgcagt ttaaacagtt caactccatt accgattacc actcgctgat
    22081 gttcgatctg ggcatcatcg cgcgtcgtct gcgctccgca tctgaccgta gcaaattcta
    22141 tcgtctgatc gaagcttcgc tgtatggcgg gatctccagt gccattaccc gttctctgcg
    22201 cgactacctg ttgccggaaa acagcggcgt gcgtaaagcg ttccaggaca tggaagcggc
    22261 gctgcgtgaa aaccgcatga cgctggaagc gattcgtgtc acccagtcgg accgcgacct
    22321 gtttaagcat ctgattagcg aagccaccaa ctacgtggcg gcggactaca tgcgtcacgc
    22381 caacgagcgc cgtgtccatc tcgataaggc cctggagttt cgtcgcgaac tacatacttc
    22441 gcgtcagcaa ctggcggcgg agcagtacaa acacgtcgat atggcgcgtg agctggcaga
    22501 gcacaacggt gccgaaggcg atctggaagc ggattatcag gcggccagcg atcacctgaa
    22561 cctggtgcaa accgcactgc gtcagcagga gaagatcgaa cgctacgaag cggatctcga
    22621 tgagctgcag atccgtctgg aagagcaaaa cgaagtggtg gcagaagcca tcgaacgcca
    22681 ggaagagaac gaggctcgtg cggaagctgc cgaactggaa gtggacgagc tgaaaagcca
    22741 gcttgctgac taccagcagg cgctggacgt ccagcaaacg cgcgcgatcc agtataacca
    22801 ggcgtgtgct gcgcttaatc gtgccaaaga actgtgccat ctgccggact taaccgccga
    22861 cagcgccgcc gaatggctgg aaaccttcca ggcgaaagag ctggaagcga ctgaaaaaat
    22921 gctcactctt gagcagaaaa tgagcatggc gcaaaccgcg cacagccagt ttgagcaggc
    22981 ttatcagctg gtggtggcaa tcaacggccc gctggcgcgt aacgaggctt gggatgtcgc
    23041 ccgcgaactg ctgcgcgaag gggtcgatca gcgtcacatg gcagagcagg ttcaaccgtt
    23101 gcggatgcgc ttaagtgaac tggaacagcg tctgcgcgag cagcaagaag ctgagcgtct
    23161 gctggcagat ttctgcaaac gccagggcaa gaattttgat atcgacgaac tggaagccct
    23221 gcatcaggag ctggaagcgc gtattgcctc tctttccgat agcgtgtcta acgcccgcga
    23281 agagcgtatg gcactgcgcc aggagcagga acagctgcag tctcgcattc agagtttgat
    23341 gcagcgtgca ccggtttggc tggcagcgca aaacagtctc aaccagttga gtgaacagtg
    23401 cggcgaagag ttctcctcca gccaggacgt cactgaatat ctgcaacagt tgctggagcg
    23461 tgagcgcgag gcgattgttg aacgcgatga agtaggcgcg cgcaaaaacg ccgtcgatga
    23521 agagatcgaa cgtttaagcc agcctggcgg ctctgaagat cagcgtctga acgcgctggc
    23581 ggagcgtttt ggtggtgtgc tgctgtcaga aatttatgac gacgttagcc tggaagatgc
    23641 gccgtacttc tcagcgctgt atggcccgtc acgccacgcc atcgtggtgc cagatctgtc
    23701 acaggtaacc gaacacctgg aaggtttgac cgattgcccg gaagatctct atttgatcga
    23761 aggggatccg cagtcattcg atgacagcgt gttcagcgtt gatgagctgg aaaaagcggt
    23821 agtggtgaaa atcgccgatc gtcagtggcg ttattcacgt ttcccggaag tgccgctgtt
    23881 tggtcgtgct gcgcgtgaaa gccgtattga aagcctccat gccgagcgtg aagtgctttc
    23941 cgaacgcttc gccacgctct cctttgatgt acagaaaacc cagcgtctgc atcaggcgtt
    24001 cagccgcttt atcggcagtc atctggcggt ggcgtttgag tctgacccgg aagcagaaat
    24061 ccgtcaactg aacagccgtc gcgtcgaact ggagcgggcg ttgagtaatc atgaaaatga
    24121 taaccagcag cagcgtattc agtttgagca ggcgaaagaa ggcgttacgg cgctgaaccg
    24181 cattctgccg cgtctcaacc tgttggctga tgacagcctg gcggatcgcg tcgatgaaat
    24241 ccgagaacgt ctggatgaag cccaggaagc cgcgcgtttt gttcagcagt ttggcaatca
    24301 actggcgaaa ctggaaccga tcgtttcggt attgcagagc gacccggaac agttcgaaca
    24361 gttaaaagaa gattacgcgt actctcagca gatgcagcgc gatgcccgtc agcaggcgtt
    24421 tgccctgacg gaagtggtgc agcgtcgtgc gcactttagc tattctgact cggcagaaat
    24481 gctgagcggt aacaacgatc tcaacgaaaa actgcgtgaa cgtctagaac aggcggaagc
    24541 ggagcgtacc cgcgctcgcg aagcgttgcg cggtcacgca gcgcagttga gtcagtacaa
    24601 ccaggtgctg gcttcgctga aaagttcgta tgacaccaaa aaagagctac tcaacgatct
    24661 gcaacgtgaa ttgcaggata tcggcgtgcg tgctgatagc ggggcagaag agcgggcgcg
    24721 tattcgccgt gacgagctgc atgcgcaact gagcaataac cgttcacgcc gaaatcaact
    24781 ggaaaaagcg cttaccttct gcgaagcgga gatggacaac ctgacccgca aactgcgcaa
    24841 gctggagcgt gattactttg agatgcgcga gcaggtagtg accgcgaaag cgggctggtg
    24901 tgcagtcatg cgcatggtga aagataacgg cgttgaacgc cgcttacacc gtcgtgagct
    24961 ggcttatctc tccgctgatg atttgcgttc catgtcggat aaggcgttag gtgcgctgcg
    25021 tctggcggtg gcggataacg aacatctgcg cgacgtgctg cgcatgtcgg aagatccgaa
    25081 acgtccggag cgtaaaattc agttcttcgt ggcggtttat cagcatctgc gtgaacgtat
    25141 tcgtcaggat attattcgta ccgatgatcc ggtggaagct atcgaacaga tggagatcga
    25201 acttagccgt ctgaccgaag agttaacctc ccgtgaacag aaactggcga tcagttcccg
    25261 cagcgtggcg aacatcattc gcaaaaccat tcagcgcgaa cagaatcgta tccgtatgct
    25321 caaccagggg ttgcagaacg tatcgtttgg tcaggtgaac agcgtacgtc tgaacgtgaa
    25381 cgtgcgtgaa acgcacgcca tgctgttgga tgtgctctct gaacagcacg agcagcatca
    25441 ggatctgttt aacagcaacc gtctgacctt ctcggaagcg ctggcgaaat tgtatcagcg
    25501 tcttaatccg cagattgata tggggcagcg cacgccgcag accattggcg aagaactgct
    25561 ggattaccgc aactatctgg aaatggaagt tgaggttaac cgtggttccg atggctggtt
    25621 gcgcgcagag tctggtgcat tgtcgaccgg tgaggcgatt ggtaccggta tgtcgattct
    25681 ggtgatggtg gtacaaagct gggaagatga atctcgccgc ctgcgcggta aagatatctc
    25741 tccttgccgc ctgctgttcc tcgatgaagc agcgcgactg gatgctcgtt ctatcgccac
    25801 gctgtttgaa ttgtgtgaac gtttgcaaat gcagctcatc atcgcagcgc cggaaaatat
    25861 cagcccggag aaaggcacca cctataaact ggtgcgtaaa gtcttccaga ataccgaaca
    25921 cgttcacgtc gtcggcctgc gaggatttgc gccgcaactc cctgaaacgc ttccaggaag
    25981 tgacgaagcg ccttctcagg cgagttaaaa ttaaggcggc agcaatgccg ccttttcttt
    26041 ttccgaaaac tccgtttctg cactaaaaaa gtggcacatt acggcgcgct tttctttaaa
    26101 cttctttaca ttaggttatg taaaaacgta tcggcgttta tatactgaag ataagcctga
    26161 tgagtaacag gcttgctcgt catactttcg tgagtattgg cgttgtacag gcaagtcgta
    26221 aaataacagc ctggctattc agagtatgat aaaaacaggg ggcaagggat gttgcttaat
    26281 atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc tggccgtaac attcgctcca
    26341 ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg gcgacagccc ggtggctgtc
    26401 agtgaacagg gcgaggcact gccgcaggcg caagccacgg caataatggc ggggatcctg
    26461 ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa tcgaatctca attacccgca
    26521 ggttacaagc cggtttatct taaccagctt caactgttgt atgccgcacg cgatatgcaa
    26581 cccatgtggg aaaaccgtga tgctgttaaa gccttccagc aacagctggc agaggtggcg
    26641 attgctggtt tccagccgca gtttaataaa tgggtagagt tactgaccga tcctggtgtt
    26701 aacgggatgg cacgtgacgt ggtgctctct gatgcgatga tgggctatct ccatttcatt
    26761 gcaaatattc cggtcaaagg cactcgctgg ctatatagca gtaaacctta tgcgctttca
    26821 acgccgccgc tttcggtgat taaccaatgg cagctagcgc tggataaagg tcaattgcct
    26881 acgtttgttg caggactggc accgcagcat ccgcaatatg cggcgatgca tgaatcgtta
    26941 ctggccttac tcagtgacac caaaccgtgg ccccaactga ccggcaaagc aacgttgcgc
    27001 ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa tattgcaacg cacaggcatg
    27061 ttggacgggg ggccgaaaat tactctacct ggcgatgaca cgccaactga cgcggtagtc
    27121 agcccatccg ctgttactgt tgaaacagca gaaactaagc cgatggacaa gcaaacgacg
    27181 tctcgtagta aacctgcgcc tgccgttcgt gccgcctacg ataatgaact ggtggaagcc
    27241 gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg gtgctattgg cccggcaacg
    27301 cgtgactggt taaacgtaac gcccgcccag cgtgctggcg tgttggcact caacatccag
    27361 cgattgcgct tgctgccaac agagctttct accgggatca tggttaacat tccggcctat
    27421 tcgctggtct actatcagaa cggcaatcag gtgctggatt cgcgagtcat tgtcggtcgc
    27481 cccgatcgca aaacgccgat gatgagcagt gcccttaaca acgtagtggt aaacccgccg
    27541 tggaacgtac caccaactct ggcacgcaaa gatattctgc cgaaagtgcg caacgatccg
    27601 ggatatctcg aaagccatgg ctatacggtg atgcgcggct ggaacagcag agaagcgatt
    27661 gacccatggc aggttgactg gtctacaatc acggcctcga atttaccgtt tcgcttccag
    27721 caggctccag gcccacggaa ctcgctgggg cgctataaat tcaatatgcc gagttcagag
    27781 gccatttatt tgcatgacac gccgaaccac aatctgttca agcgtgatac acgcgcattg
    27841 agctcaggct gtgtacgagt gaataaagct tccgatctgg cgaatatgct gttgcaggat
    27901 gcaggctgga atgacaaacg tatttctgat gcgctgaagc agggtgatac acgttacgtc
    27961 aatattcggc agtcgattcc ggtgaatctc tactacctga cggcctttgt tggtgcagat
    28021 ggtcgtaccc agtatcgtac agatatttac aattatgatc tgcctgcgcg atccagctcg
    28081 caaatcgtat cgaaagcgga acaattaatc aggtaaatga agtagttcgg ggaaatcagt
    28141 tgtcgtaata attagcatga ttgggggtga ttctctgcag cccccgtcac tgctggggtt
    28201 gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc tcttgtgcgc cagaagtgca
    28261 tataaacgat aacattgacc tgtagacttg attatcatgg acaaattcga cgctaatcgc
    28321 cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg ccatcctgcc gacccctgcg
    28381 tttgcaacac tctctacccc acgcccgcgc attttgacac tcaataatct tcataccgga
    28441 gagtcaatca aagcggagtt tttcgatggc agaggctata ttcaggaaga attggcaaaa
    28501 cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa agtccatcga cccaggatta
    28561 ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc gcaaaccggt gcaactcatt
    28621 tccggttatc gttccattga taccaacaat gaactacgcg cccgcagccg tggagtagcg
    28681 aagaaaagct atcacactaa aggccaggcg atggatttcc atattgaagg tatcgcgtta
    28741 agcaatattc gcaaagccgc gttatctatg cgcgcaggtg gtgtaggata ttacccacgt
    28801 agtaactttg tgcatattga tactgggcca gcacggcact ggtagtaatt gcttaacgaa
    28861 acaggggcag tatgaactat cgtattattc cggtcaccgc attctcccag aactgttcat
    28921 tgatctggtg tgaacaaacc cgcctggccg cactggtcga tcctggcggc gatgcggaaa
    28981 aaatcaaaca ggaagttgat gacagcggcc tgacactgat gcagatcctg ctgacgcatg
    29041 gtcatctgga ccacgttggc gcagcggcgg aactggcgca acattacggc gtgccggttt
    29101 ttggtccgga aaaagaagat gagttctggc tgcaaggctt gcctgcgcaa agtcgtatgt
    29161 ttggtctgga agagtgccag ccgctgacgc cagatcgttg gctgaacgaa ggcgatacca
    29221 tcagcatagg gaatgtgact ttacaggtgt tacattgccc tggacatacg ccgggtcatg
    29281 tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg cgatgttatt ttcaaaggcg
    29341 gagtagggcg cagtgacttc ccgcgtggcg atcataatca actgatttct tcaatcaaag
    29401 ataaattgct gccactgggg gatgacgtga catttattcc gggtcacgga ccattatcca
    29461 cacttggtta tgaacgcctg cataatccct tcctgcaaga cgaaatgccc gtctggtaag
    29521 gcacacataa aaaagcccgc ttttcagcgg gcttcattgt ttttaacgct tacagcactg
    29581 ccacaatcgc ttcgcacagc ggagccatgt tatctggtgt catcccggcc acgtttacgc
    29641 gaccagaagc aacagcatac acgccaaact cttcgcgcag acgcagcact tgttcttttg
    29701 tcaggccact gaaggagaac atgccgttct gtttgatgat aaagctgaag tcgcggtttg
    29761 cgcctttttc ctgcagcgta ttgacgaata actgacgcat acgctgaata cgctggcgca
    29821 tatcagtcag ctcttgttcc caaatcgcac gtaacgcatc gttgctcagg atggtggcaa
    29881 caacagaagc gccgtgtgct ggtgggttag agtagttagc gcgaatcgcc gctttcattt
    29941 ggctgaatgc gcgatcaacg gtttcactgt cggcagcaac cagagtacaa gcgccaacac
    30001 gctcgttgta caggccaaag tttttagagt aggaactggc aacaatcagc tctttatgca
    30061 tagctgcgaa agcgcgcagt ccttcagcat cttcttccag accacgggca aacccctggt
    30121 aagcgaagtc aaacagcggt aaccagcctt tctcaacgga gagttgtgcc agtgtctgcc
    30181 attgttccag cgtagggtcg ataccggttg ggttatggca gcagccatgg aacagcacta
    30241 catcgccagc ctgagcttcg ttcagactgt taatcagtgc atcgaagtca agggtgtgat
    30301 tttccgcgtc ataataagcg tattcacgaa cttccagacc tgcagagtta aagacgctct
    30361 tatggttcgg ccagcttggg ttgctcaccc acacacgctt aacgctggta ttttttgcca
    30421 ggaaatcggc agccacgcgt agtgcgccag tgccacccgg agtctgtgcc gtgcgagcac
    30481 gtttgtcatt gatcagggcg ctacctttac caaacagcag ttcctgagtg cagcgaccaa
    30541 attcagggat gccgtcaatg ccgaggtaat ttttggtggt ttcattttcg agcagatact
    30601 gttcagcctt tttcacgctg gtcagtaccg gggttttgcc cgtctcatct ttatagacac
    30661 caatcccgag gttaattttg ccgggacgtt catcggcacg aaacagatcg gccaggccca
    30721 gaatcgggtc ggcaggagcg gcggtaatgt tctcaaacat gacgaggttc cattatggtt
    30781 acagaaggga agtccgctat cagggtaacg ggagatttac aaaattccaa ctattactga
    30841 tgaaaacgca ggctgttttt gcaagacgtg agattgctct ggaaggcata aaaaaaacag
    30901 gaccaacgtc ctgttttttc ggcatttaac aaagaggtgt gctattagaa ctggtaaacg
    30961 atacccacag caacggtgtc gtcagaacct acgcccagtt tgttgtcaga atcgatctgg
    31021 ttgatgatgt agtcaacata ggtggacatg tttttgttga agtagtaggt tgcgcccact
    31081 tcaaagtagt tcaccagatc aacatcaccg ataccttcta cgtctttcgc tttagatttg
    31141 gtgtaagcga tggacggacg cagaccgaaa tcgaactggt attgcgcaac taacagaacg
    31201 tcttgcgttt tgttggcgaa gccgccttca attggcgtag cgttacgggt ttcaccgtag
    31261 ttcgctgcca ggtagatgtt gttcgcgtca tacttcagac cagttgccca ctgttcagct
    31321 tttttaccct gaccgcggaa ttcggcttcc tgggcgtcgg tacggtctgc tgcaccgtaa
    31381 gcacctacga taccaaagcc ttcgaattcg tagctgatag agccaccgac gccgtcgccg
    31441 ttggaacgct caggaatacc agcacgttcg tttttgccca ggtactgaac ggcaaagttc
    31501 aggccgtcaa ccagaccgaa gaagttggag ttacgatagg tagcaacgcc gcctacacga
    31561 ccaacgaaga agtcatcgct gtatgccgtg tcaccgccaa actcaggcag catatcggtg
    31621 taacccagtg cgtcgtaaac cacaccgtag ttacgaccgt agtcgataga acccgcatca
    31681 gcgaatttca gacctgcgaa tgccagacga gttttgttac ctttctgggc atcagcgcct
    31741 tcagagttgt taccctggaa gttatattcc cactgaccat aaccggtcag atcggaattg
    31801 atttgtgttt cccctttaaa accaagacgg gcataagttt tgtcgccgtt tccgccgtaa
    31861 ctgtttacac cattgtcttt agaaaaataa tgcagaccga cagctttacc gtacagatct
    31921 actttgttgc catctttgtt atagatttct gcagcgtttg cagtacctgc tactaacaga
    31981 gcagggacga tcactgccag aatattgcgc ttcatcatta tttattaccc tcatggtttt
    32041 ttttatgaca cctgccactg ccgtcaataa tttctgtcaa taaaaattta cggaactatt
    32101 gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt ccattcaaac taacgtttcg
    32161 ctaccgtgaa agtgctacaa agataaagat ttagtttcaa a
//
LOCUS       DAPGRN010000051        31812 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPGRN010000051 DAPGRN010000000
VERSION     DAPGRN010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.051"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(53..943)
                     /gene="rarD"
                     /locus_tag="RHW33_002458"
     CDS             complement(53..943)
                     /gene="rarD"
                     /locus_tag="RHW33_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV1645953.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(995..1462)
                     /locus_tag="RHW33_002459"
     CDS             complement(995..1462)
                     /locus_tag="RHW33_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV1645954.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1627..2496
                     /gene="pldA"
                     /locus_tag="RHW33_002460"
     CDS             1627..2496
                     /gene="pldA"
                     /locus_tag="RHW33_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV1645955.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2629..4458
                     /gene="recQ"
                     /locus_tag="RHW33_002461"
     CDS             2629..4458
                     /gene="recQ"
                     /locus_tag="RHW33_002461"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV1645956.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4522..5142
                     /gene="rhtC"
                     /locus_tag="RHW33_002462"
     CDS             4522..5142
                     /gene="rhtC"
                     /locus_tag="RHW33_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV1645957.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5204..5824)
                     /gene="rhtB"
                     /locus_tag="RHW33_002463"
     CDS             complement(5204..5824)
                     /gene="rhtB"
                     /locus_tag="RHW33_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV1645958.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5935..6957
                     /gene="pldB"
                     /locus_tag="RHW33_002464"
     CDS             5935..6957
                     /gene="pldB"
                     /locus_tag="RHW33_002464"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV1645959.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6965..7765
                     /gene="yigL"
                     /locus_tag="RHW33_002465"
     CDS             6965..7765
                     /gene="yigL"
                     /locus_tag="RHW33_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV1645960.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7841..8740
                     /gene="bioP"
                     /locus_tag="RHW33_002466"
     CDS             7841..8740
                     /gene="bioP"
                     /locus_tag="RHW33_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV1645961.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWSILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8628..9581)
                     /gene="metR"
                     /locus_tag="RHW33_002467"
     CDS             complement(8628..9581)
                     /gene="metR"
                     /locus_tag="RHW33_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV1645962.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQDLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9817..12078
                     /gene="metE"
                     /locus_tag="RHW33_002468"
     CDS             9817..12078
                     /gene="metE"
                     /locus_tag="RHW33_002468"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV1645963.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLDEVDEALALGHKVKPV
                     LLGPITWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12118..12933)
                     /locus_tag="RHW33_002469"
     CDS             complement(12118..12933)
                     /locus_tag="RHW33_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV1645964.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESIETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAASAEDGWQRMLEWFKQYGGKKSL"
     gene            13195..13956
                     /gene="udp"
                     /locus_tag="RHW33_002470"
     CDS             13195..13956
                     /gene="udp"
                     /locus_tag="RHW33_002470"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV1645965.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14097..15524
                     /gene="rmuC"
                     /locus_tag="RHW33_002471"
     CDS             14097..15524
                     /gene="rmuC"
                     /locus_tag="RHW33_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV1645966.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLTEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15619..16374
                     /gene="ubiE"
                     /locus_tag="RHW33_002472"
     CDS             15619..16374
                     /gene="ubiE"
                     /locus_tag="RHW33_002472"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV1645967.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16388..16993
                     /gene="ubiJ"
                     /locus_tag="RHW33_002473"
     CDS             16388..16993
                     /gene="ubiJ"
                     /locus_tag="RHW33_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV1645968.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16990..18630
                     /gene="ubiB"
                     /locus_tag="RHW33_002474"
     CDS             16990..18630
                     /gene="ubiB"
                     /locus_tag="RHW33_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV1645969.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18709..18978
                     /gene="tatA"
                     /locus_tag="RHW33_002475"
     CDS             18709..18978
                     /gene="tatA"
                     /locus_tag="RHW33_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV1645970.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18982..19497
                     /gene="tatB"
                     /locus_tag="RHW33_002476"
     CDS             18982..19497
                     /gene="tatB"
                     /locus_tag="RHW33_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV1645971.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19500..20276
                     /gene="tatC"
                     /locus_tag="RHW33_002477"
     CDS             19500..20276
                     /gene="tatC"
                     /locus_tag="RHW33_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV1645972.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20318..21100
                     /gene="tatD"
                     /locus_tag="RHW33_002478"
     CDS             20318..21100
                     /gene="tatD"
                     /locus_tag="RHW33_002478"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV1645973.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPVEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21097..21585)
                     /gene="rfaH"
                     /locus_tag="RHW33_002479"
     CDS             complement(21097..21585)
                     /gene="rfaH"
                     /locus_tag="RHW33_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV1645974.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGTFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21752..23245
                     /gene="ubiD"
                     /locus_tag="RHW33_002480"
     CDS             21752..23245
                     /gene="ubiD"
                     /locus_tag="RHW33_002480"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV1645975.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23291..23992
                     /gene="fre"
                     /locus_tag="RHW33_002481"
     CDS             23291..23992
                     /gene="fre"
                     /locus_tag="RHW33_002481"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV1645976.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24184..25347)
                     /gene="fadA"
                     /locus_tag="RHW33_002482"
     CDS             complement(24184..25347)
                     /gene="fadA"
                     /locus_tag="RHW33_002482"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV1645977.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPLNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25357..27546)
                     /gene="fadB"
                     /locus_tag="RHW33_002483"
     CDS             complement(25357..27546)
                     /gene="fadB"
                     /locus_tag="RHW33_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV1645978.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPNLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWHYKEDSKGKPKKEEDAAVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27736..29067
                     /gene="pepQ"
                     /locus_tag="RHW33_002484"
     CDS             27736..29067
                     /gene="pepQ"
                     /locus_tag="RHW33_002484"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV1645979.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29067..29681
                     /locus_tag="RHW33_002485"
     CDS             29067..29681
                     /locus_tag="RHW33_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV1645980.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29720..31171
                     /gene="trkH"
                     /locus_tag="RHW33_002486"
     CDS             29720..31171
                     /gene="trkH"
                     /locus_tag="RHW33_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV1645981.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31183..31728
                     /gene="hemG"
                     /locus_tag="RHW33_002487"
     CDS             31183..31728
                     /gene="hemG"
                     /locus_tag="RHW33_002487"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV1645982.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7368 a         8105 c         8707 g         7632 t
ORIGIN      
        1 atgcccactc tcatacaaga gtcggcatat tttttcagaa tatatttatt ttttatttgg
       61 acgttctacg ctgcgtataa atcgcatcca tcacaaaaat tgccagcgcc acccaaataa
      121 aggtgaaagt caccatctta tcggcacccg gtttttcacc ataaaacgtc acagccagca
      181 ggaacatcag cgtcgggcca atgtactgga aaaagcctaa cgttgagaga cgcagacgcg
      241 tggcagcggc agtaaaacac aacagcggta cggtagtgac aataccggcg gcgatcagca
      301 gtaaattcag cgacatcggg ttttgcccca tatggctggt tgagctgtcg gcaatagcaa
      361 acaggtaaat cgccgccacg ggcagcagcc acatggtttc aattaacatg ccggtttgtg
      421 cttctaccgc aatcttcttg cgcaccaggc cgtagaaggc aaaactaaat gccagcccca
      481 gcgcgataat aggtagcgaa ccaaaagtcc acagctggac taacacgcca catatcgcca
      541 gaatcaccgc cagccattgc atccggcgga atcgctcgcc gaggaaaatc atccccagca
      601 caatgttcac cagcgggtta ataaagtaac caaggctcgc ttccagcata tggtgattgt
      661 tcaccgccca gataaacagt agccagttgc cgccaatcag cacggcagag actgccagca
      721 taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg cggcaaatgc
      781 tcatcagcac caccataaag aaaaacgacc agatcacgcg atgcgtcagg atttcatcgg
      841 cgggcacgta gtaaatcaac ttgaagtacg ctggggctat accccaaata aaataagcgg
      901 caagagcgag taatacgccc tgccgcgttt gttttgcatc catcgggaat actcattttt
      961 aattggtaac agcagtttac ctgcttttat gtcttcaacc taccatatag gtggcggtgg
     1021 cactggcaat ataaagctgt tcttcattgt gtaattcaac gcgggcgacg gcgactttat
     1081 tgcctgcacg caacagacta ctggtagcag taaaacgctc gcccctgcct gggcgcagat
     1141 aatcaacgcg aagatcaatg gtccccatcc gcgatagccg ctggcgtagt tcatcttcac
     1201 tgatggtttc gtggcgggtt aaggtacttc ccacgcacac cagacctgcg gcgacatcca
     1261 gcgccgacgc aatgaccccg ccgtgcaaaa tgctttgcgc ccagttaccc accatcattg
     1321 gctgattttt aaaggccagc tgtgcgaact ctttttcgta acgctccagt tccatcccca
     1381 atgcgcggtt aaatggcatg tgataaacaa acatctcacc cactaatttc agggcttgtt
     1441 cagcggtcag tacggcagac atatcatcct tacacttcat tggttaatga aatgttgatt
     1501 ttatgcttct ttgttgttgg tttctacttt aggaagggat aactaacggc aatggagtta
     1561 agtatgtaaa atagcccgca gaaaaacatt caccttatca ataattcgtt acggagaaca
     1621 cgaccgatgc ggactctgca gggctggttg ttgccggtgt ttatgttgcc tatggcagta
     1681 tatgcacaag aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg tggcagtatt
     1741 atcgccaata tgctgcagga gcatgacaat ccgttcacgc tctatcctta tgacaccaac
     1801 tacctcatct acacccaaac cagcgatctg aacaaagaag cgattgccag ttacgactgg
     1861 gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga gcctggcatt tccgctgtgg
     1921 cgtgggattt taggcccgaa ctcggtgttg ggtgcgtctt atacgcaaaa atcctggtgg
     1981 caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga accgcaattg
     2041 ttcctcggtt ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga tgtggagatg
     2101 gggtataacc acgactctaa cgggcgttca gacccgacct cccgcagctg gaaccgcctt
     2161 tatactcgcc tgatggcaga aaacggtaac tggctggtag aagtgaagcc gtggtatgtg
     2221 gtgggtaata ctgacgataa cccggatatc accaaatata tgggttacta ccagcttaaa
     2281 atcggctatc acctcggtga tgcggtgctc agtgcgaaag gacagtacaa ctggaacacc
     2341 ggctacggcg gcgcggagtt aggcttaagt tacccgatca ccaaacatgt gcgcctttat
     2401 actcaggttt acagcggcta tggcgaatcg ctcatcgact ataacttcaa ccagacccgt
     2461 gtcggtgtgg gggttatgct aaacgatttg ttttgatgaa cggttgagtg gttggcaaat
     2521 ctggaatcca gcatccagga ttaccctctc agagactaaa agcattgcag tttctcgcgc
     2581 aggcgctgaa aatagcgcct gtttttattt caggcaatcg gggtgaatgt ggcgcaggcg
     2641 gaagtgttga atctggagtc cggagctaaa caggttttac aagaaacctt tggctaccaa
     2701 cagtttcgcc ccggccagga agaaattatc gacactgtgc tttccggtcg cgattgcctg
     2761 gtcgtcatgc ccaccggtgg cggaaaatcc ctttgctatc aaattcctgc cttattgcta
     2821 aacggcctta ccgtggttgt ttcaccgctg atttcgttga tgaaagatca ggtggatcaa
     2881 ctgcaagcca acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg cgaacagcaa
     2941 cttgaagtga tgacaggctg ccgcaccggg caaattcgtc tgctttatat cgccccggaa
     3001 cgcctgatgc tggataactt tcttgagcat ctggcgcact ggaatccggt gttattagcc
     3061 gttgatgaag cgcactgtat ctcccaatgg ggccacgatt tccgcccgga atatgccgcg
     3121 ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta tggcgctgac cgccacagcc
     3181 gacgacacca cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc gctgattcaa
     3241 atcagcagtt ttgaccgtcc gaatattcgc tacatgctga tggagaaatt caaaccgctc
     3301 gatcagttga tgcgctacgt gcaggaacag cgcggtaagt ccggcattat ctactgcaac
     3361 agccgggcga aagtagaaga caccgctgcg cgcctgcaaa gcaagggtat tagcgcggcg
     3421 gcctatcatg ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa attccagcgc
     3481 gatgacctgc aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa taaaccaaac
     3541 gttcgcttcg tggtccactt tgatattccg cgcaatatcg aatcctatta tcaggaaacc
     3601 ggtcgcgccg ggcgtgatgg cctgcctgcg gaagcgatgc tgttttacga tccggctgat
     3661 atggcgtggc tgcgccgttg tctggaagag aagccgcagg ggcagttgca ggatatcgag
     3721 cgccacaaac tcaatgcgat gggcgcgttt gccgaagcgc aaacttgccg tcgtctggta
     3781 ttgctgaact attttggcga agggcgtcag gagccgtgcg ggaactgcga tatctgcctc
     3841 gatccgccga aacagtacga cggttcaacc gatgctcaga ttgccctttc caccattggt
     3901 cgtgtgaatc agcggtttgg gatgggttat gtggtggaag tgattcgtgg tgctaataac
     3961 cagcgtatcc gcgactatgg tcatgacaaa ctgaaagtct atggcatggg ccgtgataaa
     4021 agccatgaac attgggtgag cgtgatccgc cagctgattc acctcggcct ggtgacgcaa
     4081 aatatagccc agcattctgc cctacaactg acagaggccg cgcgcccggt gctgcgcggc
     4141 gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa agcgatgcag
     4201 aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa actgcgtaaa
     4261 tcgattgccg atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc aaccttgatt
     4321 gagatggctg aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa cggcgttggg
     4381 atgcgcaagc tggaacgctt tggtaaaccg tttatggcgc tgattcgtgc gcatgtcgat
     4441 ggcgacgacg aagagtagtc agcagcataa aaaagtgcca ggatgaagac tccgtaaacg
     4501 tttcccccgc gagtcaaatg tatgttgatg ttatttctca ccgtcgccat ggtgcacatt
     4561 gtggcgctta tgagtcccgg tcccgatttc ttttttgtct ctcagaccgc tgtcagtcgt
     4621 tcccgtaaag aagcgatgat gggcgtgctc ggtattacct gcggcgttat ggtttgggca
     4681 gggattgcgt tgcttggcct gcatttgatt atcgaaaaaa tggcctggct gcatacgctg
     4741 attatggttg gcggtggcct gtatctctgc tggatgggtt accagatgct gcgtggcgcg
     4801 ctgaaaaaag aggcggtttc tgcacctgcg ccacaggtcg agctggcgaa aagtgggcgc
     4861 agtttcctga aaggtttact gaccaatctc gctaatccga aagcgattat ctattttggt
     4921 tcggtattct cattgtttgt cggcgataac gtcggcacta ccgcgcgctg gggcattttc
     4981 gcgttgatta ttgtcgaaac gctggcgtgg tttactgtgg tggccagcct gtttgccctg
     5041 ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg
     5101 ttatttgccg gatttggcat tcatttgatt atttcgcgtt gatgccagac gcgtcatcag
     5161 agtaagtcgg ataaggtgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg
     5221 atgctaacag cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca
     5281 tctgctttgg tcctttaatc catagagcaa tccgttgagc aagggtggcg taaccgatca
     5341 tcacaataat atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg
     5401 gctgttgcgg catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat
     5461 tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca
     5521 gcgatttaag atcaattgcg ccagcggcgc gccattgctg tattcccagc caaatcaagt
     5581 aagccgcgcc tgcccacttc aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc
     5641 ccaaccccac gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag
     5701 acgccaccgc accgcgataa ccgtggttga gcgaggtagt catggtgttg attgcgccag
     5761 aacctggcga caggcttaaa atgatcgatg tcagcagata ggcaaaccac cattctaagg
     5821 tcatgatgaa ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg
     5881 tgtcaggcac gctaaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt
     5941 cagcagcaaa aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg
     6001 ctgactgatt tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg
     6061 gtgcgctttg tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg
     6121 cgtattgaga gctacgtaaa atatgcggaa ctggcctacg atttgttcca tttggggttt
     6181 gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg
     6241 catctcgggc atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcaa
     6301 caggaggtcc agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt
     6361 gcgatctcca cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact
     6421 gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac
     6481 tgggccgaag cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc
     6541 gcgttgccgt ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta
     6601 cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc
     6661 gaaagtattc tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg
     6721 cttctcttgc aggctgaaga ggaacgcgtg gtggataacc gcatgcatga ccgtttttgt
     6781 gaactccgca ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt aattaaaggt
     6841 gcttaccatg agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatc
     6901 gttgattttt tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt
     6961 tcttatgtac caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac
     7021 gttatcccct tacgccaaag aaactctgaa gctgctcacc gcgcgcggca tcaactttgt
     7081 gttcgcgacc ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagattaa
     7141 gtcttacatg attacctcca atggtgcgcg cgttcacgat ctggatggta atctgatttt
     7201 tgctcataac ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatcc
     7261 ggacatcatt actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga
     7321 agagatgcgc ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc ctggattact
     7381 ggagccggaa ggcgtcagca aagtgttctt cacctgcgat tcccatgaac aactgctgcc
     7441 gctggagcag gcgattaacg ctcgttgggg cgatcgcgtc aacgtcagtt tctctacctt
     7501 aacctgtctg gaagtgatgg cgggcggcgt ttcaaaaggc catgcgctgg aagcggtggc
     7561 gaagaaactg ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgatgc
     7621 cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa
     7681 agaccttcat cccgagctgg aagtgattgg tactaatgcc gacgacgcgg tgccgcatta
     7741 tctgcgtaaa ctatatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact
     7801 tcgctacaat ggatactcgt taatcaaaga gttttccatt gtggcgctac ttatcatcac
     7861 cacgattctg tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga
     7921 tagctatttt gcggtgctgg tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct
     7981 gcgtacccgt ggcaatagcc tgaaaacagt cggcctgtat atgctggtgg gcgcgatgca
     8041 gctcggcgtg atgtatatgc tgagtttccg cgcttatctc tacctgacgg tttccgaact
     8101 gctgctgttc accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa
     8161 gcgccgtctg cgctggggat atgcttttag tgccttgttg gcggtgattg gtgccgggat
     8221 tattcgctat gatcaggtca ccgaccattt ctggactggc ttgttgctgg tgcaactctc
     8281 caatatcact tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat
     8341 gccgcagcat aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc
     8401 atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggagcattct
     8461 ggtgtttctt ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac
     8521 gcaggtggac gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct
     8581 ggtaaacctg gctatctggc accaacagcc gcactggcca acgtttatta caggcgcgct
     8641 ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac
     8701 ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa atgcttctgt aatcggctga
     8761 cgctgctcgc catcgcgcac ggcggcgtac agccggctcc acaagccttc gcccagggtt
     8821 ttggtcacca ccagatcctg gcgctcaaaa ctctctacta cccaatgtgg tagcgcggca
     8881 atacccatcc gcgcggcaac catctgaatc aacaataagg tgttatcgac gcttttcagt
     8941 gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc
     9001 accggataaa ttaatagcgt ctcgctggcg agatcttccg gtgtaatacg cgtttttgcc
     9061 gccagtggat ggtcaggtgc taacaccagg cgtacttcat aatcgaacat cggcgaataa
     9121 tgcaggccac tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc
     9181 aacgcgggct gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag
     9241 ttcttatgga aattttctaa cgcgggcgtc agccactgaa tacagctatg gcactcaatg
     9301 gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctaatttgc
     9361 ggcagcacct ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc
     9421 tggctcttac gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga
     9481 gacagggcgg attgcgtctg atgcaacgtc gccgcagcgg ctgcgagaga gccgcagttc
     9541 cgcaacgctt gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc
     9601 atgaataatt tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct
     9661 ggacggctaa aatccttcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc
     9721 ggtcacttac ttcagtaagc tcccggggat gaataaactt gccgccttcc ctaaattcaa
     9781 aatccatagg atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc
     9841 ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagagag ttattgggcg
     9901 gggaactcca cgcgtgaaga actgctggcg gtagggcgtg aattgcgtgc tcgtcactgg
     9961 gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat
    10021 catgtactga ccaccagtct gctgctgggt aacgttccgg cgcgtcatca gaacaaagat
    10081 ggttcggtag atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa
    10141 cctgcggcgg cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg
    10201 gagttcgtta aaggccaaca gttcaaactg acctggacgc agctgctgga cgaagtggac
    10261 gaggcgctgg cgctgggcca caaggtgaaa cctgtgctgc tggggccgat tacctggctg
    10321 tggctgggga aagtgaaagg tgaacagttt gaccgcctga gcctgctgaa cgacattctg
    10381 ccggtttatc agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt
    10441 gatgaacccg cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct
    10501 tacgacgcgc tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg
    10561 ccaaatctcg acacgattac tgcgctgcct gttcaggggc tgcatgtgga ccttgtacat
    10621 ggtaaagatg acgttgttga actgcacaag cgcctgcctt ctgactggct gctgtctgcg
    10681 ggtctgatca atggtcgtaa cgtctggcgc gccgatctta ctgagaaata tgcgcaaatt
    10741 aaggacattg tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc
    10801 cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc
    10861 gccctacaaa aatgccatga actggcactg ctgcgcgatg cgctgaacag tggtgacacg
    10921 gcagctctgg cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat
    10981 aacccggcag tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat
    11041 gtctatgaag tgcgtgccga agcccagcgt gcgcgtttta aactgccagc gtggccgacc
    11101 accacgattg gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa
    11161 aagggcaatc tcgacgccaa taactaccgc acgggcattg cggaacatat caggcaggcc
    11221 attgttgaac aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat
    11281 gacatggtgg aatactttgg cgagcacctc gacgggtttg tctttacgca aaacggttgg
    11341 gtacagagct acggttcccg ctgcgtgaag ccaccgattg ttattggtga cgttagccgc
    11401 ccggcaccga ttaccgttga gtgggcaaag tatgcgcaat ctctgactga taaaccggtg
    11461 aaagggatgc tgactggccc ggtgaccatt ctctgctggt cgttcccgcg tgaagatgtc
    11521 agccgtgaaa ccatcgccaa acaaattgcg ctggcgctgc gtgatgaagt ggcggacctg
    11581 gaggccgcag gaattggcat catccagatt gatgaaccgg cgctgcgcga aggtttaccg
    11641 ctgcgtcgta gcgactggga tgcatatctc cagtggggcg tggaggcctt ccgtatcaac
    11701 gccgccgtgg cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac
    11761 gacatcatgg attcgattgc ggcactggat gcagacgtca tcaccatcga aacctcgcgt
    11821 tccgacatgg agttgctgga gtcgtttgaa gagtttgatt atccgaatga aatcggtcca
    11881 ggcgtctatg acattcactc gccaaacgta ccgagcgtag aatggattga agccttgctg
    11941 aagaaagcgg caaaacgcat tccggcagag cgcctgtggg tcaacccgga ctgtggcctg
    12001 aaaacgcgcg gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag
    12061 aatttgcgtc gggggtaaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta
    12121 cagcgacttc ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttc
    12181 tgcagatgcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcgtccgg
    12241 gtacacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca tggtttcaat
    12301 gctctcctgc ggaatgctgt tatcctgacc gccatataag ccgagaaccg gcgcgttaag
    12361 atcggttgcg atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt
    12421 gccgtaccac gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat
    12481 acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga
    12541 agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac
    12601 cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta
    12661 aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat
    12721 atgttcatgc acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct
    12781 ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa
    12841 gcccgccaca atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc
    12901 aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg
    12961 ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct
    13021 tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgcatcactt ttggtgggta
    13081 aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc
    13141 ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc
    13201 aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc
    13261 atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt
    13321 aagctggcat ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt
    13381 atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca
    13441 cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt
    13501 aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac
    13561 ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa
    13621 gctgcgaaat ccattggcgc gacaactcac gttggcgtga cagcttcttc tgataccttc
    13681 tacccaggtc aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt
    13741 tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaaccctg
    13801 ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac
    13861 cgcacccagc aagagatccc gaatgctgag acgatgaaac aaactgaaag ccaagcggtg
    13921 aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg
    13981 acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga
    14041 cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg
    14101 atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt
    14161 ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt aactgaacgt gaagagatgg
    14221 tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt
    14281 gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg
    14341 atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc
    14401 gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta
    14461 tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt
    14521 tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta
    14581 aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg
    14641 cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc
    14701 agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag
    14761 ggtatgaata tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg
    14821 atgtcatcgt gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg
    14881 tcgcctatga acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg
    14941 aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc
    15001 tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt
    15061 tactggcgct tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc
    15121 tggttagccc gactacgctg ctggtggcgc tgcgcactat cgccaacctg tggcgttatg
    15181 agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga
    15241 tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata
    15301 actatcggca ggcaatgaaa aaactctctt cagggcgcgg aaatgtgctg gcgcaggcag
    15361 aagcgtttcg cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac
    15421 aggcggtgag ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg
    15481 aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt gggagtagtt
    15541 aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt
    15601 tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag
    15661 accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca
    15721 aaatacgatg tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc
    15781 acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc
    15841 ggcgacctga cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct
    15901 gatatcaatg aatccatgct caaaatgggc cgcgagaagc tgcgtaatat cggtgtgatt
    15961 ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt
    16021 gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt
    16081 tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca
    16141 attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt
    16201 ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg
    16261 catcccgatc aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac
    16321 tactacaatc tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag
    16381 accagaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt
    16441 cctgtatcgc tcacccgcgc tgaaaacggc ccgctcgcgt ctgttgggca aagtgctgcg
    16501 cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga
    16561 tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt
    16621 gccgaaactt cgcgatcgcc agcagcttgc cgccctgatt cgcagtggtg agctggaagt
    16681 gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga
    16741 ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc
    16801 catgcgcgga ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc
    16861 ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc
    16921 ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact
    16981 ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta
    17041 agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg
    17101 cgatactcat tattctggat gccaaatcgg cataaagaca aacctttagg tgagcgacta
    17161 cgactggccc tgcaagaact ggggccggta tggatcaagt tcgggcaaat gttatcaacc
    17221 cgccgcgatc tttttccgcc gcatattgcc gatcagctgg cgttattgca ggacaaagtt
    17281 gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg
    17341 gtagaagcgt ggtttgacga ttttgaaatc aagccactgg cttctgcttc tatcgcccag
    17401 gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg
    17461 gatattttgc cggttattaa agcggatctg aaacttatct accgtctggc tcgctgggtg
    17521 ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa
    17581 aagacattga ttgatgaact gaatttgctg cgggaatctg ccaacgctat tcagcttcgg
    17641 cgcaattttg aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt
    17701 gaagggatga tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg
    17761 gagaaaaacg gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact
    17821 caggtctttc gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc
    17881 tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta
    17941 aacaaagaag ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat
    18001 cgcaaagtgg cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa
    18061 gagttcgaat ttgctattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa
    18121 atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa
    18181 gtgcagccgc aactggtgtt actccagaaa accctgctct acgtcgaagg ggtaggacgc
    18241 cagctttatc cgcaactcga tttatggaaa acggcgaagc ctttcctgga gtcgtggatt
    18301 aaagatcagg tcggtattcc tgcgctggtg agagcattta aagaaaaagc gccgttctgg
    18361 gtcgaaaaaa tgccagaact gcctgaattg gtttacgaca gtttgcgcca gggcaagtat
    18421 ttacagcaca gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga
    18481 caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg
    18541 gtcagccgac ctgaatgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc
    18601 tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa
    18661 cgtataatgc ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc
    18721 agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag
    18781 ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc
    18841 gatgatgaac caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc
    18901 gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac
    18961 gataaagagc aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc
    19021 atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg
    19081 ggctggattc gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag
    19141 ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac
    19201 ctgacgcccg aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag
    19261 cgttcctacg ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac
    19321 ccggtggtga aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg
    19381 caggccagtt cgccggaaca gaagccagaa actacgccag agccggtggt aaaacctgct
    19441 gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca
    19501 tgtctgtaga agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc
    19561 tgaactgcat tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca
    19621 tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg
    19681 ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga
    19741 ttctgtcagc gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata
    19801 agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg
    19861 gcatggcgtt cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg
    19921 cgccggaagg ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc
    19981 tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga
    20041 tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat
    20101 tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgctg ttggcgatcc
    20161 cgatgtactg tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc
    20221 gaaatcggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc
    20281 aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt
    20341 gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg
    20401 agttaatggg ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct
    20461 ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca
    20521 gtggcaagct gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc
    20581 gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag agcaggaacg
    20641 cgcttttgtt gcccagctac gcattgccgc agatttaaac atgccggtat ttatgcactg
    20701 tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg
    20761 tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcatgg
    20821 aatttatatc ggcattaccg gttgggtttg cgatgaacga cgcggactgg agctgcggga
    20881 acttttgccg ttgattccgg tggaaaaatt actgatcgaa actgatgcgc cgtatctgct
    20941 ccctcgcgat ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca
    21001 tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac
    21061 ggatgctaat gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt
    21121 cttcacactg tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc
    21181 accatcgggt tcggtgaaaa tggcctgaaa gccttcgaac gtgccttcgg taataatcac
    21241 cttatctccc ggataagggg ttgccggatc gacaatgtct ttcggtttat ataccgatag
    21301 ctgatgaatc accgccgatg ggactatcgc tggcgacgcg ccaaagcgca cgaagtgact
    21361 gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tctggatcaa attccacaaa
    21421 caggtagttg ggaaacaatg gctcactgac tgcagtacgt tttccacgca cgattttttc
    21481 cagggtgatc atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt cctgggcacg
    21541 ttgaagttgc ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg
    21601 tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt
    21661 gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca aagatgaacg
    21721 ccgtataatg ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg
    21781 cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga
    21841 tccgcatctg gaaatcactg aaattgctga ccgcactttg cgtgccggtg ggcctgcgct
    21901 gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc
    21961 aaagcgcgtg gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa
    22021 attattggcg tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa
    22081 actgccgcag tttaagcaag tattgaacat gccgacaaag cgactacgtg gtgcgccttg
    22141 ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg
    22201 ctggccggaa gatgccgcgc cgctgattac ctgggggctg acagtgacgc gcggcccgca
    22261 taaagagcgg cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat
    22321 tatgcgctgg ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca
    22381 tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca cgattctcgg
    22441 tgcagtcact cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg
    22501 caccaaaacc gaagtagtaa agtgtatctc caatgacctt gaagtgcccg ccagtgcgga
    22561 gattgtgctg gaagggtata tcgaacaagg cgaaactgcg ccggaagggc cgtatggcga
    22621 ccacaccggt tactataatg aagtcgatag tttcccggta tttaccgtga cgcatattac
    22681 ccagcgtgaa gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc
    22741 ggtgctgggt gtcgcactga acgaagtgtt tgtgccgatt ctgcaaaaac agttcccgga
    22801 aattgtcgat ttttacctgc cgccggaagg ctgctcttat cgcctggcgg tagtgacaat
    22861 caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg
    22921 ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgttaacg cacgcgactg
    22981 gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt
    23041 agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc tgggttcaaa
    23101 aatggggctg gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc
    23161 catcaaaaaa gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat
    23221 ttttaacaac ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca
    23281 gagaaagcgc atgacaacct taagctgtaa agtgacctcg gtagaagcta tcacggatac
    23341 cgtatatcgt gtccgcatcg tgccagacgc ggccttttct tttcgcgctg gtcagtattt
    23401 gatggtagtg atggatgagc gcgacaaacg tccgttctca atggcttcga cgccggatga
    23461 aaaagggttt atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt
    23521 catggaccgc atcctcaaag atcatcaaat cgtggtcgat attccccacg gagaagcgtg
    23581 gctgcgcgat gatgaagaac gtccgatgat tttgattgcg ggcggcaccg ggttctctta
    23641 tgcccgctcg attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta
    23701 ctggggcggg cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt
    23761 gaagcatcct ggtctgcaag tggtgccggt ggttgaacaa ccggaagcgg gctggcgtgg
    23821 gcgtactggc accgtgttaa cggcggtatt gcaggatcac ggtacgctgg cagagcatga
    23881 tatctatatt gccggacgtt ttgagatggc gaaaattgcc cgtgatctgt tttgcagtga
    23941 gcgtaatgcg cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa
    24001 aaaacccgcc cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag
    24061 atctatgtcg catccggcat tcggcacacg atgcctgatg cgacgctggc gcgtcttatc
    24121 aggcctacat tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag
    24181 tggttaaacc cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc
    24241 cagaccaaac tgaacgtctt tgtgttccat cagattcagc agcgtggtgc tgatacgcgc
    24301 accggaacaa cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc
    24361 gtcaatctgc tcaattagtc ccagatcttt aatacatggc aggatctgcg cggcaaaggc
    24421 ttcattcatt tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag
    24481 cttcgaggcc ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat
    24541 cgaacgcaca cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac
    24601 cagcatggca gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt
    24661 taatggatca aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat
    24721 cacttcgtcg taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat
    24781 gatttcattt ttaaatgctg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc
    24841 ggcaaaggca tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt
    24901 taagcccatc atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac
    24961 gccgtgactc atcggcacat ggcccatatg ctccacgccg ccaaccagac atgcctgcgc
    25021 atcgccagtc atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa
    25081 gcgattgacg gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg
    25141 ggcgatgtta aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag
    25201 ggccgccgct tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag
    25261 atcttccgca cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat
    25321 tgcatcgaca atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac
    25381 cggacgggct ggctcaactg gtggatagta cggttcgtta tgacgcgctt tattacgcag
    25441 accttccggc acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta
    25501 tttcgcgctg ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc
    25561 caggccgtag accagcgcca tatccgcttc tgccggagtg gcgataatgc cttcttccag
    25621 acagcgcacc acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct
    25681 gaaatcgcgc ttcggctggc tcacttctgc cagcaggtcg tcaacagcgg cgtcttcttc
    25741 tttcttcggc ttacctttgc tgtcttcttt ataatgccag aaaccgaggc cgttcttctg
    25801 accaaagcgg ttggcatcaa acagcgcgtc gatggcatcg cgataatctt tctgcatccg
    25861 ctgtgggaag cccgctgcca tgactgcctg ggcgtgatgc gcggtgtcaa tgcccacaac
    25921 gtccagcaga tatgccggac ccataggcca gccaaactgt ttttccatca ctttgtcgat
    25981 cttgcggaaa tccgcgccgt cgcgcagcaa ctggctgaaa ccggcgaaat acgggaacag
    26041 cacgcggttg acgaagaagc cggggcaatc gttaaccaca atcggcgttt tacccatctt
    26101 gctcgcccag gcgacgactt tcgcaatagt ttcgtcggag cttttctcgc cgcgaataat
    26161 ttccaccaac ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg
    26221 acgttccagc gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac
    26281 ggtatccggg cgtactttct gctcggtttc tgccagtacg gcttttttca ctttcgggtt
    26341 ttcgacaacc gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg
    26401 gtggattgta gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg
    26461 cttgttcagc agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc
    26521 tttcatgaca accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat
    26581 gcctgcgccc agcacagcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc
    26641 tttgcctttt acatattgat cgttaaggaa tatgccgacc agtgcgcgag cttcgttggt
    26701 atgcgccagc gggacaaaac ttttgttttc caggtttaga gcttcttcac gaccaaaacg
    26761 ggccgcagct tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt
    26821 ttgtgcgacc atccctttag cgatggtgaa gctcatggta gcttcaatct tgctcagttt
    26881 tagcggttcc agcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg
    26941 gcgtaaaatc gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag
    27001 accgattttc agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc
    27061 actgtcagcg cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc
    27121 cagtttggtt tccggcagac cgatgcgcag attcggcgtc gccagacggt aatcggtcgc
    27181 cagcacgcat tcgcagccac cgcccagcgc atagccattg acggcagcaa tggtcggcac
    27241 cggcagatct tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg
    27301 ttcttcagga acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc
    27361 tgctttgttc gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat
    27421 ggcctcgccg aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc
    27481 aaataccagt tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta
    27541 aagcatgtca gtctcctgaa tccgcaaggt gatctggtcg taccagatga ctcgaagtgt
    27601 gtattttgtg ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc
    27661 ggcagaaatc acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa
    27721 acagaagggt aaaaaatgga atcactggcc tcgctttata aaaatcatat agctacctta
    27781 caggaacgga ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc
    27841 ggcgaactgt tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa
    27901 ttcaaagcgt gggtgccggt aacccaggtg ccaaactgct ggctgctggt ggatggcgtg
    27961 aacaagccga aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg
    28021 ccgacctcct tctggactga agatgtagaa gtgatcgcgc tgccgaaagc cgatggcatt
    28081 ggtagtctgt tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt
    28141 gcgctgcaac tgggtattga ggccagcaat atcaacccga aaggggttat cgactacctg
    28201 cattactacc gctccttcaa aaccgagtac gagctggcct gtatgcgtga agcgcagaaa
    28261 atggcggtca acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc
    28321 gatatcaata ttgcctatct gactgcgacc ggtcatcgtg ataccgacgt accttacagc
    28381 aacattgtgg cactcaacga acacgctgcg gtgctgcatt acaccaaact ggatcatcag
    28441 gcgccggaag agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcc
    28501 gctgacctga cccgtacctg gtcggcaaaa agtgacaacg attacgcaca gctggtgaaa
    28561 gacgtaaatg atgaacaact ggcgctgatc gcgaccatga aagctggcgt tagctatgtg
    28621 gattaccaca tccagttcca tcagcgcatc gccaaattgc tgcgtaaaca tcaaatcatc
    28681 accgatatga gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac
    28741 ggtatcggcc atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat
    28801 agcggtacgc acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc
    28861 cagccgggca tggtgttaac catcgaaccg ggtatctact tcattgaatc gctactggca
    28921 ccgtggcgtg aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa
    28981 ccgttcggcg gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac
    29041 atgacccggg atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca
    29101 cggtcgttga agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg
    29161 ttgaggcggc gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccggcacc
    29221 attgcgtggc gtgggtcgcg ggtgcgccgg atgattctca acagctgggt ttctctgacg
    29281 acggggagcc ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg
    29341 tcggggaaat taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg
    29401 ggttagtgaa agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac
    29461 gcaagacgcc attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg
    29521 aagcgttgct ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc
    29581 tgctgcgggt ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt
    29641 ttagccgtgg ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag
    29701 aactaaggaa gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt
    29761 catcttattt tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg
    29821 agcgggccgc gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctttg
    29881 gtggccgaac cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt
    29941 gctgttctgg accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc
    30001 gaacctcacg attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc
    30061 cactacgctg gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct
    30121 gcaatggttt ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg
    30181 cgtgggtggg atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat
    30241 gcgcccgcga attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt
    30301 cgcctgtgcg ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag
    30361 ctttgcgact atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga
    30421 tagcccgact attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg
    30481 tctgcacttt tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt
    30541 tcgcatgttt atcggcgtac agtttacgct ggtggttatt tgtaccctcg tactgtggtt
    30601 tcataatgtc tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtgtc
    30661 gatggcgaca accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc
    30721 ggtactgctt ttatgttcag cttttatcgg cggttgtgcc gggtcaacgg gcggtggcct
    30781 gaaagtgatc cgcatcctgc tgctgtttaa gcaggggaac cgtgaactga aacgactggt
    30841 gcatccgaac gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct
    30901 cgaagccgtt tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc
    30961 gattatcgcc acgggcgtgg atgacttttc tgcctttgcc tcggttgttg cgacattgaa
    31021 taacctgggg ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc
    31081 taaatggatc ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt
    31141 gctctttacc ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt
    31201 ttctcaacaa gggacggaca aacgcgcgag attgcctcct acttggcttc ggaactgaaa
    31261 gaactgggga tccaggcgga tgtcgccaat gtgcaccgca ttgaagaacc acagtgggaa
    31321 aactacgacc gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc
    31381 caggaatttg tcaaaaagca tgcgacgcgg ctgaattcga tgccgagcgc cttttactcc
    31441 gtaaacctgg tggcgcgtaa accggagaag cgcactccac agaccaacag ctacgcgcgg
    31501 aagtttctga tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg
    31561 cgttacccac gttatcgctg gtacgaccgt tttatgatca agctgattat gaagatgtca
    31621 ggcggtgaaa cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat
    31681 ttcgcccgag aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata
    31741 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct
    31801 ataatgcgcc ac
//
LOCUS       DAPGRN010000052        31632 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPGRN010000052 DAPGRN010000000
VERSION     DAPGRN010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.052"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..114)
                     /locus_tag="RHW33_002488"
     tRNA            complement(39..114)
                     /locus_tag="RHW33_002488"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(79..81),aa:Gly,seq:gcc)
     gene            complement(325..870)
                     /gene="orn"
                     /locus_tag="RHW33_002489"
     CDS             complement(325..870)
                     /gene="orn"
                     /locus_tag="RHW33_002489"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV1645983.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            965..2017
                     /gene="rsgA"
                     /locus_tag="RHW33_002490"
     CDS             965..2017
                     /gene="rsgA"
                     /locus_tag="RHW33_002490"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV1645984.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2113..3081
                     /gene="psd"
                     /locus_tag="RHW33_002491"
     CDS             2113..3081
                     /gene="psd"
                     /locus_tag="RHW33_002491"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV1645985.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3103..6426
                     /gene="mscM"
                     /locus_tag="RHW33_002492"
     CDS             3103..6426
                     /gene="mscM"
                     /locus_tag="RHW33_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV1645986.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6577..8079)
                     /gene="yjeM"
                     /locus_tag="RHW33_002493"
     CDS             complement(6577..8079)
                     /gene="yjeM"
                     /locus_tag="RHW33_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV1645987.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8298..9275)
                     /gene="epmA"
                     /locus_tag="RHW33_002494"
     CDS             complement(8298..9275)
                     /gene="epmA"
                     /locus_tag="RHW33_002494"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV1645988.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9600..11408
                     /gene="frdA"
                     /locus_tag="RHW33_002495"
     CDS             9600..11408
                     /gene="frdA"
                     /locus_tag="RHW33_002495"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV1645989.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11401..12135
                     /gene="frdB"
                     /locus_tag="RHW33_002496"
     CDS             11401..12135
                     /gene="frdB"
                     /locus_tag="RHW33_002496"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV1645990.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12146..12541
                     /gene="frdC"
                     /locus_tag="RHW33_002497"
     CDS             12146..12541
                     /gene="frdC"
                     /locus_tag="RHW33_002497"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV1645991.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12552..12911
                     /gene="frdD"
                     /locus_tag="RHW33_002498"
     CDS             12552..12911
                     /gene="frdD"
                     /locus_tag="RHW33_002498"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV1645992.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12974..14107
                     /locus_tag="RHW33_002499"
     CDS             12974..14107
                     /locus_tag="RHW33_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV1645993.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14196..14729
                     /gene="blc"
                     /locus_tag="RHW33_002500"
     CDS             14196..14729
                     /gene="blc"
                     /locus_tag="RHW33_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV1645994.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYHHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14726..15043)
                     /gene="sugE"
                     /locus_tag="RHW33_002501"
     CDS             complement(14726..15043)
                     /gene="sugE"
                     /locus_tag="RHW33_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1645995.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15219..15365)
                     /gene="ecnB"
                     /locus_tag="RHW33_002502"
     CDS             complement(15219..15365)
                     /gene="ecnB"
                     /locus_tag="RHW33_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV1645996.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15476..15601)
                     /gene="ecnA"
                     /locus_tag="RHW33_002503"
     CDS             complement(15476..15601)
                     /gene="ecnA"
                     /locus_tag="RHW33_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV1645997.1"
                     /translation="MMKRFIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15653..16219)
                     /gene="efp"
                     /locus_tag="RHW33_002504"
     CDS             complement(15653..16219)
                     /gene="efp"
                     /locus_tag="RHW33_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV1645998.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16261..17289
                     /gene="epmB"
                     /locus_tag="RHW33_002505"
     CDS             16261..17289
                     /gene="epmB"
                     /locus_tag="RHW33_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV1645999.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17684..18553
                     /locus_tag="RHW33_002506"
     CDS             17684..18553
                     /locus_tag="RHW33_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV1646000.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18746..19099)
                     /locus_tag="RHW33_002507"
     CDS             complement(18746..19099)
                     /locus_tag="RHW33_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV1646001.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(19237..20883)
                     /gene="groL"
                     /locus_tag="RHW33_002508"
     CDS             complement(19237..20883)
                     /gene="groL"
                     /locus_tag="RHW33_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV1646002.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20927..21220)
                     /locus_tag="RHW33_002509"
     CDS             complement(20927..21220)
                     /locus_tag="RHW33_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV1646003.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21496..22752
                     /gene="yjeH"
                     /locus_tag="RHW33_002510"
     CDS             21496..22752
                     /gene="yjeH"
                     /locus_tag="RHW33_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV1646004.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLSCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLTMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22768..23244)
                     /gene="fxsA"
                     /locus_tag="RHW33_002511"
     CDS             complement(22768..23244)
                     /gene="fxsA"
                     /locus_tag="RHW33_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV1646005.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            23580..25016
                     /gene="aspA"
                     /locus_tag="RHW33_002512"
     CDS             23580..25016
                     /gene="aspA"
                     /locus_tag="RHW33_002512"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV1646006.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            25134..26435
                     /gene="dcuA"
                     /locus_tag="RHW33_002513"
     CDS             25134..26435
                     /gene="dcuA"
                     /locus_tag="RHW33_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV1646007.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26551..26889
                     /gene="cutA"
                     /locus_tag="RHW33_002514"
     CDS             26551..26889
                     /gene="cutA"
                     /locus_tag="RHW33_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV1646008.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26865..28562
                     /gene="dsbD"
                     /locus_tag="RHW33_002515"
     CDS             26865..28562
                     /gene="dsbD"
                     /locus_tag="RHW33_002515"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV1646009.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVETKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28599..29174
                     /locus_tag="RHW33_002516"
     CDS             28599..29174
                     /locus_tag="RHW33_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1646010.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            29281..29356
                     /locus_tag="RHW33_002517"
     tRNA            29281..29356
                     /locus_tag="RHW33_002517"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29314..29316,aa:Phe,seq:gaa)
     gene            29554..30816
                     /locus_tag="RHW33_002518"
     CDS             29554..30816
                     /locus_tag="RHW33_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV1646011.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(31114..31626)
                     /gene="bmaE"
                     /locus_tag="RHW33_002519"
                     /gene_synonym="afaE-8"
     CDS             complement(31114..31626)
                     /gene="bmaE"
                     /locus_tag="RHW33_002519"
                     /gene_synonym="afaE-8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M agglutinin-type afimbrial adhesin BmaE/AfaE-8"
                     /protein_id="HDV1646012.1"
                     /translation="MNLKKIAIASSVFAGITMALTCHAVTVTATHTVESDAEFTIDWV
                     DAGPTTTDAKDGEVWGHLDMTQTRGTPTFGKLRNPQGETSPGPLKAPFSFTGPDGHTA
                     RAYLSSYGAPIHNYAGDNLANGVKVGSGSGNDPFVVGTTSRLTAKIFGDQTLVPGVYR
                     TTFELTTWTD"
BASE COUNT         7785 a         8237 c         7949 g         7661 t
ORIGIN      
        1 gcttgggtgg tctgtgcttt gcagcacttt tcaaattttg gagcgggaaa cgagactcga
       61 actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc ccgcgtacta
      121 cttaattttg ctttcgtaat tttcgcattt ctgcgtcgtt acgggaggcg cattatacga
      181 gaaatctttc cctctgcaac ccccctaaaa aatttttttt gagtttttat ctcaagtgct
      241 gaaattatca gcaaggcgtt caatttagcg tcaaaatcac gccgccgcaa gtggattccg
      301 gcgcttacgt gaccagcaaa aattttacag cttgataaaa tgctcgcggt agtaagccag
      361 ctccgccacc gattcacgga tatcatccat cgcctgatgc gtcccctgct tggtaaaacc
      421 atcaagaatt tccggcttcc agcggcgcgc cagctctttc agggtgctga catcgagata
      481 acggtagtgg aagtaggctt ccagctccgg catgtattta aacaggaagc gacgatcctg
      541 accgatgctg ttaccgcaaa tcggcgattt tcccgcaggc acccactgtt ttaagaattc
      601 gagcgttgcc agttcagctt cacgatcgcc catcgtgctc gctttcacgc gctccaccag
      661 cccgctggcg gtatgggtgc gcacgttcca gtcatccatc agcgccagct gttcatcaga
      721 ctggtgtact gcaatggtcg gcccttctgc cagaatattc aggttggcat cggtcaccag
      781 cgtggcaatc tcaataatgc gatcgcgctc gggatccaga ccggtcatct caagatcgat
      841 ccaaatcagg ttgttttcat tggcactcat gctattttcc acccattcac agtgactata
      901 ttgatccaca attgcgtgta tcatagatgt tttgcccatc aggggcgacc aggagtctgt
      961 acgattgagt aaaaataaac tctccaaagg ccagcagcgc cgcgtgaacg ccaatcacca
     1021 gcgtcgtctt aaaacgtcta aggagaagcc cgactacgac gacaatctgt ttggcgagcc
     1081 tgatgaaggt atcgtcatca gccgctttgg tatgcacgct gatgtggaat ccgccgatgg
     1141 cgacgttcac cgctgcaata ttcgccgtac catccgttcg ctggtgaccg gcgaccgcgt
     1201 agtctggcgt ccgggtaaac cggcggcgga aggcgtaaat gtcaaaggga tcgtggaagc
     1261 ggtgcatgag cgcacctcgg tgttaacacg cccggatttc tatgacggcg tgaaacccat
     1321 cgccgccaac atcgaccaga ttgtcattgt ctccgccatt ttgccggagc tgtcactcaa
     1381 tattatcgac cgttacctgg tggcctgcga aaccttgcag attgagccga ttattgtgct
     1441 caacaagata gacctgctgg acgacgaagg catggcgttc gtcaacgaac agatggatat
     1501 ctaccgcaat atcggttatc gcgtattgat ggtttccagc catactcagg atgggctaaa
     1561 accgctggaa gaggcgttga ccgggcgcat cagcattttt gccgggcagt ctggcgtcgg
     1621 caaatccagc ctgctgaacg cgttgctggg gctgcaaaaa gagatcctga ccaacgatgt
     1681 ctctgacaac tcggggctcg gtcagcacac cacgaccgcc gcacgcctgt atcacttccc
     1741 tcacggcggt gatgtgattg attcccctgg tgtgcgtgag ttcggcctct ggcacctgga
     1801 gccggaacaa atcactcagg gctttgtcga attccatgac tatttaggtc tgtgtaaata
     1861 tcgcgattgc aaacacgata ccgatccggg ctgcgctatc cgggaagcgg ttgaggaagg
     1921 aaaaatcgcg gaaacccgtt tcgaaaacta tcaccgtatt ctggaaagca tggcgcaggt
     1981 aaaaacgcgt aaaaactttt ctgatacgga tgactgacaa ttaagctaac cgtcgttaga
     2041 atcgtcccct tttttcagga tccggcatgt aggccggatc aggaacgaca aaacaatggc
     2101 ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac agtacattct gccgaaacta
     2161 tggcttactc gcctggcggg ttggggcgca agcaagcggg caggatggct gacaaaactg
     2221 gttatcgatc tgttcgttaa atactacaag gtcgacatga aagaggcgca aaagccggac
     2281 accgccagct accgcacctt taacgaattc tttgtccgtc cgctgcgtga cgaagtacgc
     2341 ccaatcgata ccgatccgaa tgtcctggtc atgcctgccg atggcgttat cagccagttg
     2401 ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc acaactacag cctcgaagcc
     2461 ctgctggcag gcaactatct gatggcggac ctgttccgca acggtacgtt tgtgaccact
     2521 tacctctccc cgcgtgacta ccaccgcgta cacatgccgt gcaacggtat tctgcgtgag
     2581 atgatctacg tgccgggcga tctcttctcc gttaaccatc tcacggctca gaacgtgccg
     2641 aatctgtttg cccgtaacga acgcgtgatc tgcctgttcg ataccgaatt tggcccaatg
     2701 gcgcagattc tggtcggggc gacgattgta ggcagcattg agactgtctg ggcgggcacc
     2761 attacgccgc cgcgcgaagg catcatcaag cgctggacct ggcctgccgg ggaaaacgac
     2821 ggttctgtgg cactgctgaa aggccaggaa atgggtcgct ttaaactcgg ttccaccgtt
     2881 atcaacctgt ttgcaccagg taaagtgaat ctggttgagc aactggaaag cctgtctgtc
     2941 acgaaaattg gccagccgct ggcagtatct accgaaacct ttgttacgcc agacgctgaa
     3001 cccgccccgc ttcctgctga agagatcgaa gcagaacacg acgccagccc attggttgac
     3061 gataaaaaag accaggtcta atccatcaaa ggaaacgctg acgtgcgcct gattatcact
     3121 tttctgatgg cctggtgcct cagttggggg gcgtacgccg cgacggcccc cgatagcaaa
     3181 caaatcaccc aggaactgga gcaggcaaaa gcggcgaaac ccgcgcagcc agaagtcata
     3241 gaggcactcc agtctgcctt aaatgcgctt gaggaacgga aaggttccct tgagcgcatc
     3301 aagcaatatc aagaagttat tgataattat ccgaaactct ccgctactct gcgcgcacaa
     3361 ttaaacaaca tgcgtgacga gccgcgcagc gtgtcgccgg ggatgtctac cgacgcgctg
     3421 aatcaggaaa ttctccaggt cagcagtcag ttgctggata aaagccgtca ggcccagcaa
     3481 gagcaggagc gcgcccgcga gattgccgat tcgctgaatc aactgccaca acagcaaacc
     3541 gacgcccgcc gtcagttaaa cgagatcgag cgccgcctgg ggacgcttac cggcaatact
     3601 ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact ctgcacgtct taaggcgctc
     3661 gttgatgaac tggagctggc gcagctgtct gccaataacc gccaggaatt agcgcgctta
     3721 cgctcagagc ttgcggaaaa agagagccag caactggatg cgtatttgca ggccttacgt
     3781 aatcaattaa acagccaacg tcagcttgag gcggagcggg cgctggaaag taccgaattg
     3841 ctggcagaaa acagtgccga tttgccgaaa gacatcgtcg cgcaattcaa aattaaccgc
     3901 gaactgtcgg cggctttgaa tcaacaggcg cagcggatgg atctcgttgc ctcgcaacag
     3961 cgtcaggctg ccagccagac gttacaggtc cggcaggcgt tgaatacgct gcgtgaacag
     4021 tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt tgcgggcgca ggtggcacgg
     4081 ctgccggaaa tgccgaaacc acaacagctt gataccgaaa tggcgcagtt gcgtgtgcaa
     4141 cggttacgtt atgaggatct gcttaataaa cagccgctgc tacggcaaat tcatcaggcc
     4201 gacggtcagc cgctgactgc cgagcaaaac cgtattctcg aagcacagct acgcactcag
     4261 cgtgagttgc tgaactcatt gttgcagggt ggcgacacac tactgctgga actgaccaag
     4321 ctgaaagtct ccaacgggca actggaggat gcgctgaaag aggtgaacga agcgacgcac
     4381 cgctatctgt tctggacctc tgacgtgcgc ccgatgacca tcgcctggcc gctggaaatc
     4441 gcccaggatc tgcgtcgtct catttcgctg gacaccttca gtcagttggg caaagccagt
     4501 gtgatgatgc tgaccagcaa agagacgatt ttgccgctgt ttggcgcgtt gattctggtc
     4561 ggttgcagta tttactcgcg ccgctatttt acccgttttc tcgaacgttc ggcggcgaaa
     4621 gtcggcaaag tgactcagga tcacttctgg ctgacgttgc gcactctttt ctggtcgatt
     4681 ctcgtcgcgt caccgctgcc ggtgctgtgg atgacgctgg gttacggctt gcgcgaggcg
     4741 tggccttatc cgctggcggt cgcgattggc gatggcgtaa cagccaccgt gccgctgctg
     4801 tgggtagtga tgatttgcgc tacttttgcc cgcccgaacg gcttgtttat cgctcatttt
     4861 ggctggccgc gcgaacgtgt ttctcgtggg atgcgctact acctgatgag catcgggctt
     4921 attgtgccac tgattatggc gctgatgatg ttcgataacc tcgacgaccg cgaattttcc
     4981 ggttcgctgg gacggctttg ctttatcctc atttgcggtg cgctggcggt ggtcaccctc
     5041 agcctgaaaa aggccgggat accgctgtat ctcaacaaag agggcagcgg cgacaacatt
     5101 accaaccata tgctgtggaa catgatgatt ggcgcgccac tggttgccat tctggcgtcg
     5161 gcggtgggtt atctggcaac ggcgcaggca ctgttagcga ggcttgaaac ctcggttgcc
     5221 atctggttcc tgctactggt ggtttatcac gttatccgcc gctggatgct gatccagcgc
     5281 cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag agatgttagc gcaacgcgcg
     5341 cgtggcgaag aggaagcgca tcatcacagt agcccggaag gggcaattga agtcgatgaa
     5401 agcgaagtcg atctcgatgc cattagtgcg caatccttgc ggctggtgcg ctcaattttg
     5461 atgttgatcg ccctgctttc ggtcattgtg ctgtggtcag aaatccattc cgctttcggc
     5521 ttcctcgaaa atatttcgct gtgggatgtc acctccacgg tacagggcgt agaaagtctg
     5581 gagccaatta ccctcggtgc ggtgctgatt gccattctgg tgtttatcat caccacgcaa
     5641 ctggtgcgca acctgcccgc gctgctggaa ctggcgattt tgcagcacct ggatttaacg
     5701 ccgggtacgg gttacgccat caccaccatc accaaatatc tgctgatgct gattggcggg
     5761 ctggtcggct tctcaatgat tggtattgag tggtcgaaat tgcagtggct ggttgccgcg
     5821 ctcggtgttg gtctcggttt tggtttgcag gaaattttcg ccaactttat ctctggcctg
     5881 atcatcctgt tcgaaaaacc gattcgcatt ggcgatacgg taacgatccg cgatcttact
     5941 ggtagcgtga caaaaattaa cacccgcgcc accaccatca gcgactggga ccgtaaagag
     6001 ataatcgtgc cgaacaaggc gtttattacc gagcagttta tcaactggtc gctctctgac
     6061 tcggtcacgc gcgtggtatt gacgattccg gcccctgccg atgccaacag tgaagaagtg
     6121 acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg tgatcgacaa cccggcaccg
     6181 gaagtcttcc tggtggatct gcaacagggg attcagattt tcgagctgcg tatttacgcc
     6241 gctgagatgg gtcaccgtat gccgctacgc catgagatcc accagctgat tctggctggc
     6301 ttccatgccc acggtatcga tatgccattc ccgcccttcc agatgcgtct ggaaagcctc
     6361 aacggtaaac aaacggggag aacgctgacg tctgcgggca aaggtcgtca ggcgggaagt
     6421 ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc atttagcagc ttaacatcat
     6481 cttcagttat caccggccct ggagagtatc atccatacgc attacttcct gacaactcat
     6541 cgccggagag acgatgagtt gcaggatttc atatgatcag tcgagagcta actcaggttt
     6601 attcgccatg cgactgcaat agttctggta aatcgccatc gctaacagcg agaagaagac
     6661 agggccgcca atcatccaca atgtgctgtc ccagtctccg gcttcaacca caggctgaat
     6721 aatggtgaag acgttcgcaa atgtcaccac cagaacgaca accaccgtcg caatcattgc
     6781 cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga tcctgacgtg ctttaaagaa
     6841 tgggaaagcc agcgcgagga acaggtaagg aagcgtcata gacacgttcg ccatcagcgt
     6901 cagcttgtta aagaacgccg atgcggtacc gccaccaaac gaaaccagca ggatgaagac
     6961 agtaaccaac ccgcactgca tccacatggc gatagacggc atccccatcg cattcaggcg
     7021 cgtcatcggt tccggccaca acgctttcgg cgtcccctgg atgatggctt tcagcggtga
     7081 atagcaaagc gtaaagaacg caccggtata agcgaggaac atcgaaagtc cggtaatacg
     7141 cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac aaatgcagtg cgttacccag
     7201 cgtcaccccg aggctcttca tcagcacata ggtaatattg ccaaggttaa cggaaccatt
     7261 acttaatacc tgctgccagt ttgtgctgac gccccataaa aatattgcca gcgaataacc
     7321 gattgaaata acaatagcgg caaaaacaat acctttggca aagttctttt ctggattttc
     7381 cgttttatcg accagaccac caaccgcttc aattccgcca taggcaaaaa tagcaaatac
     7441 cacaaacgat agcattgcca gaccggactg ataacccggg ttcggtgatg caaggaaatt
     7501 aatatcctgc gcgaaatgcc caccatttaa taacaaaata gtaatgctta ctaacagcaa
     7561 tactaaattc agacacatta ctgcaatacc gcccaccgca gtaatgcggg caattttatt
     7621 tatcccctta gaagcaacga cggtgaccag aatcatccat gccactgcca gcagaccaac
     7681 cacctgcgta ggctccagtc cggcaatacg ccagtgctgg gtcatgtcgc taccatagag
     7741 gaatgttgag aacggtaccc aaactttcgc ggaggtactc accatccaga tgatataaga
     7801 ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt ccgacactat tattcatcca
     7861 ggaatagata ccaccttctt ctttgcgata agcagctccc atttcagcca tcattaaggc
     7921 gaatggaata aagaataaca atgcagaaaa tatataaaag ggaatcgcac tataacccat
     7981 taagtaataa gccgatgggc tattggcaaa tccaaatacg gaagtaaaga tcatcaatat
     8041 gagtcctatc agactcatct tttttatcgt gtgaggcatg aaaccatcct tcatttcagc
     8101 gcgtgtataa aacgcgccca ctgaactgca ctgcccaaca ttgagcgggc agcgtaaaaa
     8161 ccaatacaga attgatacct ggatgatatc aaatatccag tcttaaattg tggcctttag
     8221 cggaaaaatg aaaacatatg ctacagaatg gcgcttatca cgccattctt cgctgttaat
     8281 tcagtaattt ttcagaatta tgcccggtca acgctaaagg cgatgacttc agccagtgtc
     8341 tccgccccca gcgccaacat caccagacga tcaacgccta atgccacgcc ggaacagtca
     8401 ggcataccga ctttcaaggc ttcaatcaga ttctggtcaa tggggtgctg cggcaaaccg
     8461 cgcgccgcgc gcttacggtt atcttgttca aagcgttgtt gctgctcacg ggcatccgtc
     8521 aattcatgga aaccattcgc cagctcaata cctttataat aaacctcaaa gcgttcagcg
     8581 acccgatgat cttcggtact gatttgcgcc agtgatgcct ggctggctgg aaagtggtac
     8641 acaaaggtcg gtttttcttt accaatattt ggctctacgc caaaggtaaa cagcaattgt
     8701 agcaacgtgt cgcggtcttc ttcggtatca gcaacattgc tcaaatccag tttcgctgcc
     8761 acttcccgca gttgcgtttt gtcggcagag agtgggtcaa tttccagata acgcaagaaa
     8821 gcttgttgat aagaaaggct ttctgctgcc gggcagtcca gtacctgttg taagaggtcg
     8881 tccacctcgt tcatcaaccg gtacatatca tagtgcggtc gataccactc aagcatagtg
     8941 aactcagggt tgtgataacg ccccatctct tcattacgga agctgcggca cagctggaat
     9001 accggcccac aaccagcaac cagcaggcgt ttcatatggt attcagggct ggtcattaac
     9061 cagagattca tcccctgcga atgcccgggg ccaacgaaac gtgtctcaaa cgggaccaaa
     9121 tgaatatcgg ttaccgtcgc ctggctcata caaggcgtct ccacctccag cactccacga
     9181 tcggcaaaga aacgacggat ctccgccata atcgccgcgc gttttaataa gttaggaatg
     9241 gatgcgctcg gctgccagga tgccgtttcg ctcatagtta aatctccagt ttttgacaag
     9301 ggcacgaagt ctactcgcaa cgcgacggcg agacaaattt tacgcaggaa tcaaacaacg
     9361 gtgggcagtg actaaaaaaa gcacgatctg atggtttagt aattaaatta atcatcttca
     9421 gtgataattt agccctcttg cgcactaaaa aaatcgatct cgtcaaattt cagacttatc
     9481 catcagacta tactgttgta cctataaagg agcagtggaa tagcgttcgc agaccgtaac
     9541 tttcaggcac ttaccctgaa gtacggggct gtgggataaa aacaatctgg aggaatgtcg
     9601 tgcaaacctt tcaagccgat cttgccattg taggcgccgg tggcgcggga ttacgtgctg
     9661 caattgctgc cgcgcaggca aatccaaatg caaaaatcgc actaatctca aaagtatacc
     9721 cgatgcgtag ccataccgtt gctgcagaag ggggctccgc cgctgtcgcg caggatcatg
     9781 acagcttcga atatcacttt cacgatacag tagcgggtgg cgactggttg tgtgagcagg
     9841 atgtcgtgga ttatttcgtc caccactgcc caaccgaaat gacccaactg gaactgtggg
     9901 gatgcccatg gagccgtcgc ccggatggta gcgtcaacgt acgtcgcttc ggcggcatga
     9961 aaatcgagcg tacctggttc gccgccgata agaccggctt ccatatgctg cacacgctgt
    10021 tccagacctc tctgcaattc ccgcagatcc agcgttttga cgaacatttc gtgctggata
    10081 ttctggttga tgatggtcat gttcgcggcc tggtagcaat gaacatgatg gaaggcacgc
    10141 tggtgcagat ccgtgctaac gcagtcgtta tggctaccgg cggtgcgggt cgcgtttatc
    10201 gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg ctaagccacg
    10261 gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg ccaggttccg
    10321 gtatcctgat gaccgaaggt tgccgtggcg aaggcggtat tctggtcaac aaaaatggct
    10381 accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag ccgaaaaaca
    10441 aatatatgga actgggtcca cgcgataaag tctctcaggc cttctggcac gaatggcgta
    10501 aaggcaacac catctccacg ccgcgtggcg atgtggttta tctcgacctg cgtcacctcg
    10561 gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa gcgtacgttg
    10621 gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac cgcacactac accatgggcg
    10681 gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc gtgggtgaat
    10741 gttcctctgt tggtctgcac ggtgcaaacc gtctgggttc taactccctg gcggaactgg
    10801 tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact gccggtaatg
    10861 gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg aaagatctgg
    10921 ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc ctggctatgg
    10981 aagaaggctg cggtatttac cgtacgccgg aactgatgca gaaaaccatc gacaagctgg
    11041 cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc gtattcaaca
    11101 ccgacctgct ctacaccatt gaactgggcc acggtctgaa cgttgctgaa tgtatggcgc
    11161 actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac gaaggttgca
    11221 ccgagcgtga cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat gctgatggca
    11281 cgactcgcct ggagtacagc gacgtgaaga ttactacgct gccgccagct aaacgcgttt
    11341 acggtggcga agcggatgca gccgataagg cggaagcagc caataagaag gagaaggcga
    11401 atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga agtcgatacc
    11461 gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt actggatgcg
    11521 ctgggctaca tcaaagacaa cctggcaccg gacctgagct accgctggtc ctgccgtatg
    11581 gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc atgtaaaacc
    11641 ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt cccgattgaa
    11701 cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat caaaccgtac
    11761 atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc ggcgcagatg
    11821 gccaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc cgcgtgcccg
    11881 cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc gcatcgttat
    11941 aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa cagccagaac
    12001 ggcgtatgga gctgtacttt cgtgggctac tgctccgaag tctgcccgaa acacgtcgat
    12061 ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct tatcgcgacc
    12121 ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg tacggccaat
    12181 gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc gcgaaggcac
    12241 ggcggttccg gctgtgtggt tcagcattga actgattttc gggctgtttg ccctgaaaaa
    12301 tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta ttgtgatcat
    12361 taacctgatc actctggcgg cagctctgct gcacaccaaa acctggtttg aactggcacc
    12421 gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa ttatcaaaag
    12481 tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc tgtactggta
    12541 aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta ttctggggcc
    12601 tcttcggggc cggtggtatg tggagcgcca tcattgcgcc ggtgatgatc cttctggtgg
    12661 gtattctgct gccactgggg ctgtttccgg gtgatgcact gagctacgag cgcgttctgg
    12721 cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt ctgccgctgt
    12781 ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaatccac gtacctgcgg
    12841 gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg attggtgtcg
    12901 ttacaatcta acgcatcgcc aatgtaaatc cggcccgcct atggcgggcc gttttgtatg
    12961 gaaaccagac cctatgttca aaacgacgct ctgcgcctta ttaattaccg cctcttgctc
    13021 cacatttgct gccccccaaa aaatcaacga tattgtgcat cgcacaatta ccccgcttat
    13081 agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta aaccttatta
    13141 ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc aaacgttgtt
    13201 tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgacg ctattgctcg
    13261 aggggaaatc aagttaagcg atcccgcaac aaaatactgg cctgaactta ccgctaaaca
    13321 gtggaacggg atcacgctat tacatctcgc aacctacacc gctggcggcc tgccattgca
    13381 ggtgccggat gaggtgaaat cctcaagcga cttgctgcgc ttctatcaaa actggcagcc
    13441 tgcatgggct ccaggaacac aacgtctgta tgccaactcc agtatcggtt tgttcggcgc
    13501 actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaactc gtgtcttcca
    13561 gccactcaaa ctcaaccata cgtggattaa tgtaccgccc gcagaagaaa agaattacgc
    13621 ctggggatat cgcgaaggta aggcagtgca tgtttcgcca ggggcgttag atgctgaagc
    13681 ttatggtgtg aagtcgacca ttgaagatat ggcccgctgg gtgcaaatca atttaaaacc
    13741 ccttgatatc aatgagaaaa cgcttcaaca agggatacaa ctggcacaat ctcgctactg
    13801 gcaaaccggc gatatgtatc agggtctggg ctgggaaatg ctggactggc cggtaaatcc
    13861 tgacatcatc gttaacggca gtgacaataa aattgcactg gcagcacgcc ccgtaaaagc
    13921 gattacgccc ccaactcctg cagtacgcgc atcatgggta cataaaacgg gggcgaccgg
    13981 cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatcg tgatgctggc
    14041 taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tggcagattc tcaacgctct
    14101 gcagtaaaat ttcatcgggt ccgaattttc ggaccttttc tccgcttttc cttgctgtca
    14161 tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct ctcgttgccg
    14221 cagcgacagc tgcatttctg gtcgttgcct gcagttctcc tacgccgccg cgtggcgtga
    14281 ccgtagtgaa taatttcgac gccaaacgtt atctcggtac ctggtatgag attgcccgtt
    14341 ttgatcaccg ctttgaacgt ggactggaaa aagtcaccgc aacatacagc ctgcgtgatg
    14401 acggcggcct gaatgtcatt aataaaggct ataaccctga cagaggaatg tggcagcaga
    14461 gtgaagggaa agcgtacttt accggcgcac caactcgcgc tgcgctgaaa gtgtcattct
    14521 ttggtccttt ctatggcggt tataacgtta ttgcactcga tcgggaatac caccatgcgc
    14581 tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca accatttctg
    14641 acgaagtgaa acaagagatg ctggcagtcg cgacccggga agggtttgat gtcagtaagt
    14701 ttatttgggt acagcagccc ggtagttaat gagtgctgag tttcagacca ataatcccca
    14761 atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcaccg agcagcacaa
    14821 tgccggtgat ggccgcgccg actgcgccaa tacccgtcca cacggcataa gccgtcccta
    14881 ctggtaacga tttcatcgcc caggcaagta gcgccatact gacgatcatc gccgtcacag
    14941 taataacact tggcgtcaaa cgactaaagc cgtgggtata tttcaggcca acggcccata
    15001 ccacttccag cagaccagca ataactaaga taatccagga catatcaggc tccggagaat
    15061 tggggccgtc cccggtgaaa agacgcgttt gcaggtcgtc ctgcaaagct gatggttgaa
    15121 cggatatttt gccaggtggc aggggaattt caactattac ctttgtgact atcgcagaaa
    15181 aaacaaacgg cacgacacag ctgtcgtacc gttattgctt attgctgcgc tttcgttgct
    15241 gcaccagaaa tcgcgttacc gccatcagaa atgtcttcac caacgccacg cgtggtgttg
    15301 caggcagtta atactgttga aagcaccaga acagaaaaga tcgctgcaat tgtcttcttc
    15361 accataacgt cttcctttta gataatgtta tttatgtttg cctatagcaa attaataata
    15421 gacaacatcg ccaaaaatga cggaaatctg atgatttttc ggaggagaag aaaaattagc
    15481 tggcagcgcg ggagatggag ttgccgagat gtttgatgtc ttcgccgaaa ccgcgagcgg
    15541 tgttacagcc cgtgagcagc gtgctggcaa gcaaaacaag aacgataaag cgtttcatca
    15601 tctctgacct gaataagtga tggtgcagcc tgtaggccgc accacaaccg cattacttca
    15661 cgcgagagac gtattcacca gagcgggtat ccactttgat gacttcgccg atttgtacga
    15721 acagcggaac tttaaccaca gcgccagtag acagggtagc cggtttgccg ccagtacctg
    15781 cggtatcacc tttcaggccc ggatcggtat caacgatttc cagttcaacg aagttcggcg
    15841 gagtaacgga gatcggctga ccattccaca gagttacgat acactctgcc tgatccagca
    15901 gccatttagc gttgtcgcca attgcttttg cgtcagcaga cagctgctcg aaagtttcgt
    15961 tgttcatgaa gtgccagaac tcaccgtcgt tgtacaggta agtcaggttc atatcgacaa
    16021 catcagcgcc ttcagcggaa tcagtagatt tgaaggtttt ttctacgcga gtaccggtca
    16081 gcagacgacg cagtttaacg cgagcaaatg cctggccttt acccggtttt acgaattcac
    16141 tcgcttcaac cgcgtaaggt tcgccgtcta acatgatttt aagaccagca cgaaaatcgt
    16201 tgctatagta cgttgccata aggccctctg aaatttgtta attggtagct aagccacaaa
    16261 atggcgcata ttgtaaccct aaatacccca tccagagaag attggttaac gcaacttgcc
    16321 gatgttgtga ccgatcctga tgaacttctg cgtcttttga atatagacgc ggacgaaaaa
    16381 ctgttggccg gacgcaacgc caaaaagctg tttgccctgc gtgtgccccg ctcatttatc
    16441 gatcgcatgg agaaaggcaa tccggacgat ccgcttttgc gtcaggtact tacctcgcaa
    16501 gatgagtttg ccatcgcgcc cggattctcc accgacccgc tggaagaaca gcacagcgta
    16561 gtgcctggtt tgttgcataa ataccacaac cgggcgcttt tgctggtcaa aggcggctgc
    16621 gcggtaaatt gccgctattg cttccgtcgc cacttcccct atgccgaaaa tcagggcaac
    16681 aagcgtaact ggcaaactgc acttgagtat gttgctgcgc atccggaact ggacgagatg
    16741 attttctccg gcggcgatcc gctgatggcg aaagatcacg agctggactg gttgctcaca
    16801 caactggaag ccatcccgca tatcaaacgt ctgcggattc acagccgtct gccgattgtg
    16861 atcccggcac gtatcaccga ggcgctggtt gaacgctttg cccgttctac gctgcaaatc
    16921 ttgctggtga atcacatcaa tcatgccaat gaggtagatg aaacattccg tcaggcgatg
    16981 gctaagttgc gccgggtagg cgttactttg ctgaaccaga gcgttctgtt acgtgatgtg
    17041 aacgataacg cacaaacgct ggcaaacctg agtaatgcgt tgttcgatgc cggcgtgatg
    17101 ccgtattacc tgcatgtgct cgataaagta cagggcgcgg cgcattttat ggtgagtgat
    17161 gacgaagcac ggcagattat gcgtgagttg ctgacactgg tgtcgggata tctggtgccg
    17221 aaactggcgc gagaaattgg cggcgaaccc agcaaaacgc cgctggatct ccagctacgc
    17281 cagcagtaac tgattaaaca gggaagtcaa acattaaaat tgcctgatgc gctacgctta
    17341 tcaggcctac atcgtgcatt gaaattgaat ggatttgcaa tgaactgtag gccggataag
    17401 gcgtttacgc cgcatccggc aagagcaaca aacgcaagta cagcaacctg tcattaaaca
    17461 gggaagttta atctttgccc gttgcataac ctttaattat tcatatgcat tgctgttgtt
    17521 actctttata taactaacac caataattaa cctttctttt ctgtttatat agagtgagta
    17581 cggctattta tgttatttta acaactatta ctaccaagcc catttatctg gcttataaat
    17641 taaaattaat tatttaattg agaaatggtt agggagaacc tacatggcca taagcatcaa
    17701 gggtgttaat actggtgtta ttcgcaagag taataatttc attgcactgg cgctgaaaat
    17761 taaagaacct cgcaataaag aatcgttgtt ctttatgtct gtcatggaac tgcgcgacct
    17821 gctgattgcg ctggaaagtc gtctgcatca aaagcataaa ctggatgctg ctgcacgcct
    17881 tcagtatgag caagctcgcg ataaagtcat taagaaaatg gcagaaaaca tcccagaaat
    17941 tctggttgat gagcttaaaa atgccgatat taatcgtcgg gttaacacgc tggaacttac
    18001 cgataatcaa ggcgaaaatc ttaccttcgt attaacgcta catgacggta gcaaatgtga
    18061 gttagtcgtc aatgagttgc agattgaaat gctggcgcga gcaatcattc atgcaattaa
    18121 taatgcggaa atgcgtgaac tggcattacg tattacctca ctgttagatt tcctgccgtt
    18181 atatgacgtt gactgccagc aaaatggcaa tctggaatat gacacctatt cccaaccgga
    18241 atggaaacat aacttgttcg accattatct ggcggtgctt taccgcttta aagatgaaag
    18301 tggcaaagaa caatttagcg gcgcagtggt gaaaacgcgt gaagcaacgc cgggtaaaga
    18361 aatagaggcg atcactcgcc gaatgcttga tttcagccca cgactgaaaa aactcgtggg
    18421 tgtgccatgt caggtctatg ttcgcaccgt agcggcgaat aacgcccaac cgctaaccca
    18481 ggatcaatgc ttacgcgcgc tgcatcactt acgtgttcaa tccaccagca aaactgcacc
    18541 acaggcgaaa taatctcatc tttgctaatg cgaggctgga gccatccagc ctgaaatgac
    18601 tgataacgtg atcgttggtc atgccggatg cggcgtaaac gccttatccg gcctacaaat
    18661 tctcaagcat tcaatcagtt gcaatgcctt atgtaggcct gataagcgaa gcgcatctgg
    18721 caattatacg tttgtcacca tcacatcagt tcgggcactt atatacctgg ccgataatct
    18781 ggctatccgt cgggacaaaa ctggacaaca ttccctgcga cgggctactg atgccataaa
    18841 tcacgttacc gcccattgca gccgcctggt tgcgcagatc gtttgctgcg ccgcgcatag
    18901 aaccgccttc ttctccgtgt tgcccggaaa gccagttgct ttgcttacct gtcgcagtac
    18961 caatcagctg gcactctgcg cctggctgct cgtccacaat gcgtacactc tgaccggcag
    19021 cactcaattc gttgctggag ctacaacccg ccatcagtag cgcggcaccg acaatccctg
    19081 ctaagtattt tacgtgcatg ttattcccca taatcaatga gctggacgcc actcgcgtcg
    19141 tccgtgtctg aatcttatac tagaaagatg accaaaagaa aaacccccag acatttctgc
    19201 ccgggggttt gtttatttct gcgaggtgca gggcaattac atcatgccgc ccatgccacc
    19261 catgccgccc ataccgccag cagcgcctaa gtcagctgca tcatttttcg gcaggtcggt
    19321 aaccatgcac tcggtggtga tcatcaggcc agccacagaa gccgcgtact gcagagcaga
    19381 acgggttact ttggttgggt ccaggatacc catgtcgatc atgttgccgt attcttcggt
    19441 tgctgcgttg taaccgtagt tgccgtcgcc gcctttaacg gtgttagcaa caacagacgg
    19501 ttcttcgccg cagttcagga cgatctgacg cagcggagct tccattgcac gcagtgcaac
    19561 tttgataccc acgttctggt cttcgttctg accacgcagg tcagccagtt tagacgctac
    19621 gcggatcagc gcaacaccac caccagcaac cacgccttct tctaccgcag cacgggtcgc
    19681 gtgcagggcg tcttcaacgc gtgctttttt ctctttcatt tcaacttcgg tagcagcacc
    19741 tactttgata actgcaacgc cgcctgccag tttcgctacg cgctcctgca gtttttcacg
    19801 gtcgtagtca gaagttgctt cttcaatctg ctgacggatc tgagcaacac ggccctggat
    19861 tgcagcttct tcgcccacgc catcgatgat ggtggtggtg tctttgttga tcacaacgcg
    19921 tttagcctga cccaggtctt ccagggttgc tttttccagc tccataccga tctcttcaga
    19981 gattacggta ccgccagtca gggttgcgat atcctgcagc atagctttac gacgatcgcc
    20041 gaagcccgga gctttaactg cagcaacttt cacgatgcca cgcatggtgt taacaaccag
    20101 agttgccagc gcttcgcctt ctacatcttc agcgatgatc agcagcggtt tgcctgcttt
    20161 ggcaacggct tccagaaccg gcagcatttc gcggatgttg gagattttct tgtcagccag
    20221 caggatgaac gggctttcca gttctactgc gccagtttcc ggcttgttga tgaagtaagg
    20281 agacaggtag ccacggtcga actgcatacc ttcaaccacg tccagttcgt cctgcagacc
    20341 ggtaccgtct tcaacggtga taacgccttc tttaccgact ttgtccatcg cttcagcgat
    20401 cagtttacct acggtttcgt cggagttagc ggagatagta ccaacctgag caatcgcttt
    20461 agagtcagag cacggtacgg acagcgcttt cagttcttca actgcagcgg taacagcttt
    20521 gtcgatacca cgtttcaggt ccatcgggtt catgcccgca gcaacagctt tcagaccttc
    20581 agtgatgata gcctgagcca gtacggttgc agtggtggta ccgtcgcctg cagcgtcgtt
    20641 cgctttagag gcaacttctt tcaccatctg cgcacccata ttttcgaact tgtcttccag
    20701 ttcgatttca cgagcaacgg aaacaccatc tttggtgatg gtcggtgcac cgaaagattt
    20761 atccagaact acgttacggc ctttcggacc gagggtaact ttcactgcat ctgccagtac
    20821 gtttacgccg cgcagcattt tcacacgagc gtcgttaccg aattttacgt ctttagctgc
    20881 cattatcttt attccttaaa ttcgtatgtt cagtgtcgtg cgaggattac gcttcaacaa
    20941 ttgccagaat gtcgctttcg gacatgatca acacttcttc attgtcgatc ttctcggatt
    21001 tcacaccgta gccatcgttg aaaataacga tgtcgccaac tttcacgtcc agcggcttca
    21061 cttcgccatt ttcaaggata cggccattgc cgacagccag cacttcgccg cgggtggatt
    21121 tagccgctgc agagccggtc agaacgatgc cgccagcaga tttagtttca acttctttac
    21181 gcttgacgat cacgcgatca tgcaatggac gaatattcat tgataactct cctttgagaa
    21241 agtccgtatc tgttatgggt gacgccggag cttacgtggt ttcccggctg gtgaccagag
    21301 aaatggggat gaggcttcgc cccttcaagg gggaaaaaga aaaaaaattc tgatcagcac
    21361 aaaatcgggt gaaaaccctg attcacctca catttcatcg caatttcttc atcgccgtca
    21421 taagcgaatc tgattgtgct accatcgaaa atctacgcag ttgcccaaaa tttgggcgca
    21481 atcggacatc aacccatgag tggactcaaa caagaactgg ggctggccca gggcattggc
    21541 ctgctatcga cgtcattatt aggcactggc gtgtttgccg ttcctgcgtt agctgcgctg
    21601 gtagcgggca ataacagcct gtgggcgtgg cccgttttga ttatcttagt gttcccgatt
    21661 gcgattgtgt ttgcgattct gggtcgccac tatcccagtg caggcggcgt cgcgcacttc
    21721 gtcggtatgg cgtttggttc gcggcttgag cgagtcaccg gctggctgtt tttatcggtc
    21781 attcccgtgg gtttgcctgc cgcgctacaa attgcagccg gattcggcca ggcgatgttt
    21841 ggctggcata gctggcaact gttgttggca gaactcggta cgctggcgtt ggtgtggtat
    21901 atcggtactc gcggtgccag ttccagtgca aatctacaaa ccgttattgc cggacttatc
    21961 gtcgcactga ttgtcgctat ctggtgggcg ggcgatatca aacctgcgag tatccccttc
    22021 cccgcgccag gaaatatcga acttaccggg ttatttgctg cgttatcagt gatgttctgg
    22081 tgttttgtcg gtcttgaagc atttgcccat cttgcctcgg aatttaaaaa tccagaacgt
    22141 gattttcctc gtgctttgat gattggtctg ctgctggcag gattagtcta ctggggctgt
    22201 acggtagtcg tcttacactt cgacgcctat ggtgaacaaa tggcggcggc cgcatcgctt
    22261 ccaaaaattg tagtgcagtt gttcggtgta ggagcgttat ggattgcctg cgtgattggc
    22321 tatctggcct gctttgccag tctcaacatt tatatacaga gcttcgcccg cctggtctgg
    22381 tcgcaggcgc aacataatcc tgaccactac ctggcacgcc tctcttctcg ccatatcccg
    22441 aataatgccc tcaatgcggt gctcagctgc tgcgtggtga gcacgttggt gattcatgct
    22501 ttagagatca atctggacgc tcttattatt tatgccaatg gcatctttat tatgatttat
    22561 ctgttatgca tgctggcagg ctgtaaatta ttgcaaggtc gttatcgact actggcggtg
    22621 gttggcgggc tgttatgcgt tctgttactg acaatggtcg gctggaaaag tctctatgcg
    22681 ctgattatgc tggcggggtt atggctgttt ctgccaaaac gaaaaacgcc ggaaaatggc
    22741 ataaccacat aatccggcgt ttcgacatta atcctggcga tcgtctttat gatcaaggcg
    22801 gtcgcgctca tcatcctttc gctggtactc accatcaaaa gtattaccgc caccggtccc
    22861 ggcgctaaaa ccgccgcctg gcatgcgaga aaagcgcaaa tgtggcatca acttcactgt
    22921 cagatgcttt tgcaccggcg gcaataaaag cagaagaccg aggaagtcgg tgaaaaagcc
    22981 cggtaataaa agcagcaaac cggcaatgat cagcgaaaca cttttaatca tctccgccgc
    23041 tgggttttca cccgccgcca ttttttgctg catcagcacg aaattcttaa agccctggtt
    23101 acgtaccagt gacataccga taactgacgt gaatataacc agcacgaggg tcagcaatac
    23161 ccccaataca tgtgcaacct gaataaagat tgaaatctca atatagacat aaaggaaaat
    23221 ggcaataaaa ggtaaccagc gcaaaggttt ctcctgtaat agcagccggt taacccggct
    23281 acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga
    23341 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata
    23401 attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag
    23461 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    23521 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    23581 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    23641 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    23701 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    23761 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    23821 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    23881 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    23941 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    24001 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    24061 ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    24121 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    24181 gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaac atccaacgta
    24241 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    24301 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    24361 gcgtaccggc tgaagacctg atcgaagcga cctcagactg cggcgcttat gttatggttc
    24421 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    24481 tctcttctgg tccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct
    24541 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    24601 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    24661 tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac attctgacca
    24721 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    24781 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    24841 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    24901 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    24961 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    25021 acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgagcagt
    25081 aacttaaaaa taacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    25141 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    25201 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    25261 gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    25321 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    25381 acccgaaata catcacgatc ctcgcaccga tcgtgaccta tttcctgact atctttgctg
    25441 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    25501 gcgttaaacc ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt gcgatcaccg
    25561 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    25621 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    25681 ccttcctggt cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc
    25741 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    25801 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    25861 tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc
    25921 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    25981 acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    26041 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg
    26101 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    26161 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    26221 tttctccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga
    26281 cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    26341 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    26401 gcttcggctt cgtgctgggt agcttcatgc tgtaatgacc aatcgcgggg cgttcacgcc
    26461 ccgctttctt tcccgccgac taacatcctt cccccgtccg ttgtatagtg acctctctct
    26521 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg
    26581 tctgtcgtgg tgctatgtac cgcaccggat gaagcgacag cccaggattt agccgccaaa
    26641 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    26701 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    26761 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    26821 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    26881 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    26941 gacgttcaca atttgtcccc gcggatcaag cctttgcttt tgattttcag caaaaccaac
    27001 atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    27061 gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg
    27121 aagatgagtt ttacggcaaa agtgagattt accgcgatcg gctgacgctt cccgtaacca
    27181 tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg
    27241 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    27301 cagcgtcaca gcctgtgtct gttccgcagc aagagcagca caccgcgcaa ttgccctttt
    27361 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    27421 tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag
    27481 cattgttgct gacctttatt tatgtgcagg ggatggctct gacttacacg gcgctgggtc
    27541 tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    27601 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac
    27661 aactcccctc ctcgctgcaa acgcgcctca cgctgatgag caatcgccaa cagggcggct
    27721 cacccggcgg agtgtttatt atgggggcga ttgccggact gatctgttca ccatgcacca
    27781 ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg
    27841 gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct
    27901 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    27961 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatatatggg
    28021 gattacgctt gtggtcggcg cttggtgtcg cattctttgg ctgggccttt atcaccagcc
    28081 tacaggccaa acgcggctgg atgcgcgtgg tgcaaataat cctgcttgca gcggcattgg
    28141 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga
    28201 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcaggcg ctcgttgaaa
    28261 ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt
    28321 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagctgac acggtcttac
    28381 ttcaggccaa cgtcacggcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    28441 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    28501 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    28561 gaacgacact tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    28621 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    28681 ctgaacgtgt ggattatcca ctggacgagc tgcgccgctt ctggccagac aaagaggcaa
    28741 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    28801 tgctggacga aacgcaaacc gccgaacaaa agctgctggc acgttatcag gcgttatcgg
    28861 agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc
    28921 ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    28981 tcacccacga actgttaacc acgctggaag ttgacgatcc ggcgatggta gcaaagcaga
    29041 tggaactggt gctggaaggt tgcctgagcc gaatgctggt gaatcgtagc caggcggatg
    29101 tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    29161 gcgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    29221 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    29281 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    29341 ccgagtccgg gcaccaaatt catatcaacg gacctccacg gaggtccgtt tttcgtttca
    29401 gaacaccatg atttaagtgt tcttcctcca aatcaattct accgaactca accaaattct
    29461 ccccacatca acctcattgt gtgggtataa ttgcgggcat accccagttc gacagaattt
    29521 gtaccctctt taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg
    29581 gctgcaaagc ccactgacaa ggcttataaa ctcactgacg gggccggcat gttcctgctg
    29641 gttcatccta atggttcccg ttactggcgt ctccgttatc gtattctggg taaggagaag
    29701 actctggcac ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat
    29761 gaggcccgaa aactgatttc agaggggatt gacccttgcg aacagaaaag agttaaaaaa
    29821 gttgtccctg atttacagct ctcttttgaa catattgcac gacgctggca tgccagtaat
    29881 aaacaatggg cacaatcaca cagcgataaa gtactcaaaa gcctcgagac acacgttttc
    29941 ccctttatcg gcaaccggga tatcacaaca ctcaataccc cggacctgct tatccctgtt
    30001 cgtgctgcag aagcaaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct
    30061 gctgtaatgc gttacgccgt acagtctggc atcatcagat ataacccggc tctggatatg
    30121 gctggtgcat tgaccacggt aaaacgccag catcgccctg ctctggagct ttcacgcctg
    30181 cctgaacttc tgtcgcgtat tgacagttat aaaggccagc ctgtcacccg actggcagtt
    30241 atgctgaatt tactggtttt tattcgttcc agtgaactca gatatgcccg ctggtcggaa
    30301 attgatattg acaattccat gtggactatt ccagccgaac gcgaacctct gccaggcgta
    30361 aaattctcac accggggatc caagatgcga acaccacatc ttgtgccact cagcaaacag
    30421 gttgtagcca tactggcaga acttcagaca tgggctggtg aaaatggtct gatctttacg
    30481 ggagcacatg acccacgtaa accaatcagt gaaaacaccg taaataaagc cctgagggtt
    30541 atggggtatg acacaaccca ggatgtctgt ggtcatggat tccgggcgat ggcatgtagt
    30601 gcgttaatag aatcaggttt gtggtcccgc gatgcagttg aacgtcagat gagccatcag
    30661 gaacgtaacg gtgtacgtgc tgcttacatt cataaagcag aacatctgga agaacggcga
    30721 ctgatgctac agtggtgggc agattttctg gatgcgaaca gagaacaatg tatcagcccg
    30781 tttgaatatg caaagattaa caatccatta aaatagtaat catcccgggc aaatgcccgg
    30841 gaacctactc caggattatt ttctttgtta aaaaaacaga caaaacggta ttaacttcgt
    30901 aagcgtacag cgagggcctt attagcgagg aagatttctc ttgtaggtag taatgtaacc
    30961 caaagataac ggcttcttta aacttttggg cagcaacatt tataatctca tggctacttt
    31021 tcactgccag gctgatttaa acacaaccat tttatgctgg taaatcaaca aagggagcat
    31081 atagccccct tctttcatca ggttaatttt ccatcagtcg gtccaagtag ttaattcaaa
    31141 ggttgtccgg tagactcctg gaaccaatgt ctggtctccg aagatttttg cagttagtcg
    31201 acttgttgtc ccaacaacaa atggatcgtt tccgcttcca ctacctacct tcaccccatt
    31261 agcaaggtta tcccctgcgt agttgtgaat cggtgcgccg tatgaactaa ggtacgctct
    31321 tgcagtatga ccatctggcc cggtaaaact gaatggcgcc ttcaacggtc ctggcgaagt
    31381 ctctccttga ggattgcgga gttttccgaa tgttggtgtt cccctggttt gagtcatatc
    31441 aaggtgcccc caaacctcac catcttttgc atctgtagtc gttggcccag cgtcgaccca
    31501 atctattgtg aattcagcat ctgattcaac tgtatgagtg gctgttactg tcacagcatg
    31561 gcaagttagc gccattgtta tacctgcaaa gacactgctt gcgatagcta ttttctttaa
    31621 attcatacct ta
//
LOCUS       DAPGRN010000053        31303 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPGRN010000053 DAPGRN010000000
VERSION     DAPGRN010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.053"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(294..369)
                     /locus_tag="RHW33_002520"
     tRNA            complement(294..369)
                     /locus_tag="RHW33_002520"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:Asn,seq:gtt)
     gene            complement(470..2113)
                     /gene="yeeO"
                     /locus_tag="RHW33_002521"
     CDS             complement(470..2113)
                     /gene="yeeO"
                     /locus_tag="RHW33_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV1646013.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2118..2193
                     /locus_tag="RHW33_002522"
     tRNA            2118..2193
                     /locus_tag="RHW33_002522"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2151..2153,aa:Asn,seq:gtt)
     gene            complement(2231..3181)
                     /gene="cbl"
                     /locus_tag="RHW33_002523"
     CDS             complement(2231..3181)
                     /gene="cbl"
                     /locus_tag="RHW33_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV1646014.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFREIFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3283..4200)
                     /gene="nac"
                     /locus_tag="RHW33_002524"
     CDS             complement(3283..4200)
                     /gene="nac"
                     /locus_tag="RHW33_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV1646015.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4526..4601
                     /locus_tag="RHW33_002525"
     tRNA            4526..4601
                     /locus_tag="RHW33_002525"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4559..4561,aa:Asn,seq:gtt)
     gene            complement(4657..5592)
                     /gene="ldtA"
                     /locus_tag="RHW33_002526"
     CDS             complement(4657..5592)
                     /gene="ldtA"
                     /locus_tag="RHW33_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV1646016.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5654..6733)
                     /gene="cobT"
                     /locus_tag="RHW33_002527"
     CDS             complement(5654..6733)
                     /gene="cobT"
                     /locus_tag="RHW33_002527"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV1646017.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLFDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6745..7488)
                     /gene="cobS"
                     /locus_tag="RHW33_002528"
     CDS             complement(6745..7488)
                     /gene="cobS"
                     /locus_tag="RHW33_002528"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV1646018.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREERLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7485..8030)
                     /gene="cobU"
                     /locus_tag="RHW33_002529"
     CDS             complement(7485..8030)
                     /gene="cobU"
                     /locus_tag="RHW33_002529"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV1646019.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAIHAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8164..8523)
                     /locus_tag="RHW33_002530"
     CDS             complement(8164..8523)
                     /locus_tag="RHW33_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDV1646020.1"
                     /translation="MHCRGCGFIREDWQQDFQPLNGGSVQSLSDAYSDYSVQLLIAAE
                     SEISVPEWLPHSEGTTVILIAEHNARLLTGPAGGAVALIHYLNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            8502..8798
                     /locus_tag="RHW33_002531"
                     /pseudo
     CDS             8502..8798
                     /locus_tag="RHW33_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization protein"
     gene            complement(8899..9228)
                     /locus_tag="RHW33_002532"
     CDS             complement(8899..9228)
                     /locus_tag="RHW33_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV1646021.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9400..10458)
                     /locus_tag="RHW33_002533"
     CDS             complement(9400..10458)
                     /locus_tag="RHW33_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV1646022.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(10657..11130)
                     /gene="sbmC"
                     /locus_tag="RHW33_002534"
     CDS             complement(10657..11130)
                     /gene="sbmC"
                     /locus_tag="RHW33_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV1646023.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(11249..12415)
                     /gene="dacD"
                     /locus_tag="RHW33_002535"
     CDS             complement(11249..12415)
                     /gene="dacD"
                     /locus_tag="RHW33_002535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV1646024.1"
                     /translation="MKRRLIIAASLFVFTLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDEIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            12624..14051
                     /gene="sbcB"
                     /locus_tag="RHW33_002536"
     CDS             12624..14051
                     /gene="sbcB"
                     /locus_tag="RHW33_002536"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV1646025.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENNDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGSLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(14094..14321)
                     /locus_tag="RHW33_002537"
     CDS             complement(14094..14321)
                     /locus_tag="RHW33_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV1646026.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(14335..15393)
                     /gene="yeeE"
                     /locus_tag="RHW33_002538"
     CDS             complement(14335..15393)
                     /gene="yeeE"
                     /locus_tag="RHW33_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV1646027.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWVGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(15572..16930)
                     /gene="plaP"
                     /locus_tag="RHW33_002539"
     CDS             complement(15572..16930)
                     /gene="plaP"
                     /locus_tag="RHW33_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV1646028.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(16920..16982)
                     /gene="yoeI"
                     /locus_tag="RHW33_002540"
     CDS             complement(16920..16982)
                     /gene="yoeI"
                     /locus_tag="RHW33_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV1646029.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(17197..18126)
                     /locus_tag="RHW33_002541"
     CDS             complement(17197..18126)
                     /locus_tag="RHW33_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1646030.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(18172..18996)
                     /locus_tag="RHW33_002542"
     CDS             complement(18172..18996)
                     /locus_tag="RHW33_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1646031.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            19423..19473
                     /gene="hisL"
                     /locus_tag="RHW33_002543"
     CDS             19423..19473
                     /gene="hisL"
                     /locus_tag="RHW33_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV1646032.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    19449..19572
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            19619..20518
                     /gene="hisG"
                     /locus_tag="RHW33_002544"
     CDS             19619..20518
                     /gene="hisG"
                     /locus_tag="RHW33_002544"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV1646033.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGITFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            20524..21828
                     /gene="hisD"
                     /locus_tag="RHW33_002545"
     CDS             20524..21828
                     /gene="hisD"
                     /locus_tag="RHW33_002545"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV1646034.1"
                     /translation="MSFNIIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            21825..22895
                     /gene="hisC"
                     /locus_tag="RHW33_002546"
     CDS             21825..22895
                     /gene="hisC"
                     /locus_tag="RHW33_002546"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV1646035.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAMIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFCTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            22895..23962
                     /gene="hisB"
                     /locus_tag="RHW33_002547"
     CDS             22895..23962
                     /gene="hisB"
                     /locus_tag="RHW33_002547"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV1646036.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            23962..24552
                     /gene="hisH"
                     /locus_tag="RHW33_002548"
     CDS             23962..24552
                     /gene="hisH"
                     /locus_tag="RHW33_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV1646037.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            24552..25289
                     /gene="hisA"
                     /locus_tag="RHW33_002549"
     CDS             24552..25289
                     /gene="hisA"
                     /locus_tag="RHW33_002549"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV1646038.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNNPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            25271..26047
                     /gene="hisF"
                     /locus_tag="RHW33_002550"
     CDS             25271..26047
                     /gene="hisF"
                     /locus_tag="RHW33_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV1646039.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKTYLATQGVEIRIC"
     gene            26041..26652
                     /locus_tag="RHW33_002551"
     CDS             26041..26652
                     /locus_tag="RHW33_002551"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV1646040.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLTTVIENLRKRHQ"
     gene            complement(26749..27729)
                     /gene="wzzB"
                     /locus_tag="RHW33_002552"
     CDS             complement(26749..27729)
                     /gene="wzzB"
                     /locus_tag="RHW33_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV1646041.1"
                     /translation="MRVENNNVSGQNLDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVFDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIGPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     VELEKDLKDNIALRMKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTGEDITQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIH
                     AYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(27876..29042)
                     /gene="ugd"
                     /locus_tag="RHW33_002553"
     CDS             complement(27876..29042)
                     /gene="ugd"
                     /locus_tag="RHW33_002553"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV1646042.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTIPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(29289..30695)
                     /gene="gndA"
                     /locus_tag="RHW33_002554"
     CDS             complement(29289..30695)
                     /gene="gndA"
                     /locus_tag="RHW33_002554"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV1646043.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILIKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(30813..>31303)
                     /locus_tag="RHW33_002555"
     CDS             complement(30813..>31303)
                     /locus_tag="RHW33_002555"
                     /inference="COORDINATES: protein motif:HMM:NF025072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV1646044.1"
                     /translation="NADAARFLCNEIYPSLQSMYMDVKVYIVGKNPPNDLLDSKNIIR
                     TGFVSDVSVYYRMATVFICPLKLGAGMKNKILEAMATGLPIISSDVGVEGIDGLTDGE
                     NYLRANTLHEYVDAITRVHNNPLLGDGLGRKAAKLISEKYDWQNIVDRYVTKLQDISD
                     TQ"
BASE COUNT         8314 a         8147 c         7372 g         7470 t
ORIGIN      
        1 agaaggcaag tggtaattac gtatagtttc ccgataaaga cacgacagca acatcccgca
       61 taaggcattg ttctctttat ctttatacgg tacttcatgg aacagagatt ttgaccttgc
      121 gaatcgtgat gtctgttggg ggcaatttgc tcactgaagc gtgagactcg attaagcgca
      181 cgaaacacag aaatcaaaaa acccggtcac ttttttacaa ggtaaccggg taaaaataat
      241 ttttattttt ttaactattt tgagactcat agagatgtct caaaactaaa atttggctcc
      301 tctgactgga ctcgaaccag tgacatacgg attaacagtc cgccgttcta ccgactgaac
      361 tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc
      421 tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca
      481 caactggctt tttttcacac ttttgcggct cggatcgagg gtatttccat agccaacgtc
      541 cagtaaccat tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca
      601 tccagacacc aaccaccccc cagccaagca tgatgcccag cacataaccg acgacaaccc
      661 ggcaacccca catgctcaac atagaaaccc acatagcgta acgggcgtca cgagccccct
      721 taaatccagc gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagcc
      781 aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac gatgccataa
      841 ccccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg gaaagccaga
      901 acacatgccg caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg
      961 taatgatcgt tgaagcagag ccgagcgcac ttccgggtaa gttgataaga gccgcaattg
     1021 aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg aacatttggg
     1081 ttaataaccg accactggta aataacactg attcgacact cgcgggaata ccaatcccca
     1141 tgacttccca gataatgcta aaattcagtg gcttaaaata gctctttaac gaaatcctta
     1201 gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag
     1261 aaatggttaa acccaaccca gccccgacaa atcccagtcc cggccaggag aaaaggccgt
     1321 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcagtagcg
     1381 gtattttcgt attccctgca ccacgaagtg ccccgctacc aattagagtg atggcagctg
     1441 ctggataact gagtaccgtc agctccagat aagtcaaagc cagtgcttta acttctgtcg
     1501 tggcatcacc cgcgacgaaa tcaataattt gctcgccaaa atgatgaata agcgttgcca
     1561 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     1621 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag
     1681 caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat
     1741 cttttcccag ccagctgacc agaaaagtgc tcaggacacc catcaacagg acacaggcat
     1801 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
     1861 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     1921 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag
     1981 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     2041 acggcaaaca gttggggaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
     2101 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     2161 atgtcactgg ttcgagtcca gtcagaggag ccaattttct gaaaagcccg cttttatagc
     2221 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctggcgct
     2281 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
     2341 gctgaagttg tcctcgcttg agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     2401 tatccaggcg gattaaattc ttttcctctt gttcaccgct ggattgttcg gcaactaatc
     2461 cgatccccag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     2521 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatgcgtgag cgccccgtaa
     2581 taccctgtcg gtaagtgatt aatggccact tcgctattga ttccagcgtc aatggtgtaa
     2641 tttgcgtcaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga
     2701 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     2761 cattttgcaa caacgtcgca atttcctgtg gcgtcccctg gattagctcg agccgaacct
     2821 ccgggaaaat ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     2881 gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     2941 gaacattact ggcttcattg agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     3001 cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatcct
     3061 ccagttcccg aatatgacgg ctgacgcctg actgtgaggt aaaaagcata ttcgcaacct
     3121 ctgtcaggtt gtaatcctga cgtgcagctt cgcggattat ctttagttgt tggaaattca
     3181 cggtaaactc cgggcagttc agatatcccg ttattgttaa agtctaacgc ccggcataac
     3241 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaattgcca
     3301 ctgccttttt tccatcactg gagaactaat cactgacatt aacaactctt tcactgcctg
     3361 tgcctgtggc gataagttcg ctctggcggg taaatttaat gacaaagaga gactcattga
     3421 aggcgtggta atacgtgaca tccacccatt tactgcgcca cataacgaac gcgcggccga
     3481 ttcgggtaat actgcaacgc ccatgccgct ggcaatcgct gcggtaagcg tggcaataga
     3541 ctcaatttca ccaatgactt ttgccgtgag tcgccgtagg gaaaacgcct catcaacacg
     3601 aagtctaaca gcgctgtaat cactggggag aaagaggttc atttgtgcaa tagcattaac
     3661 atcaacgctt tgccccgggc aatcttgagt tcctaccagg aaaagatctt ctttcagcaa
     3721 agcctgactg gataccccag ccacagggga atgctcataa atcaccgcca tatcgagttg
     3781 gtgatttatc agtttttcgt taagcactgc accgctattt tcatgaagat agataacgat
     3841 ctccgggaat tcagcgcgaa ccgcttgtaa taagggcatg gtgatagatg acgcagcggt
     3901 tcctggcgca aagccaatcg agacttgccc cgataatgcc tgaccaacgt tatgcaccgc
     3961 cagttgggcc tgctcacact gacgtaaaat ggcccgcgca tgggtataga gaatttttcc
     4021 ggcgtctgtt ggcgtaacgc cccgttttgt gcgaatcaaa agttgttgat ttaactcacc
     4081 ttcgagtgtg gcaacctgct ggctgagcgc tggttgtgcg atatgcaata cttcagcagc
     4141 ctgagtcagg ctaccaatat ctacaatttt tacgaagtat ttcaggcgtc tgaagttcat
     4201 gttgcctccg gtttttgaga atcggcccaa gtgccgccat tacttacaac cagactgcaa
     4261 gatgcttgcc agttttattt tggcgttgat gtacaagcta accaactgtc aaataagaga
     4321 ttatgataga ttcgtcattg gctcttttca ccagcagtcg cgttcccctg ccctataaaa
     4381 ggagggtatg caccacgatg gttcattacc aataagattg aaatctcatc actttgttga
     4441 aattggcagc aaacaaacaa aaaaatgcat ttcacccttt gacatcacca tgcactgcca
     4501 ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt
     4561 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aagcctgctt
     4621 tctagcaggc tttttgcttt ctaattacca actctcttaa aacatctgtc ttgaaccaga
     4681 actaatttgc acaggcattc ccgatcgacg ttgcaacgca gcatttgcgc gattcacatc
     4741 aacttcttgc ccgttgataa acgcccgcaa agatggggtt accggcaatg gcacttttcg
     4801 gtcagactca tattctgcac gattgcgcga caacggctca tgaacttcca gccagttcga
     4861 gccatctggt tcagtggtgt attttactgg ctggtcgata atttgcacac gcgtcccaac
     4921 aggaacatta tcaaacagat atttgatatc gtcattgcgc agacgaatac agccctgact
     4981 tacccggagc ccaataccaa aattggcatt ggtaccgtgg atggcataca acctgccaat
     5041 ataaatcgcg tacagcccca tgggattatc gggacccgca ggaacaaatg cgggcaaact
     5101 ctcccctcgt ttcgcatatt cgcgccgagt gttcggcgtt ggcgtccagg ttggagcttc
     5161 ttgtttacgt tcaacggtag tcacccagtt acgcggggtt tctcgcccgg cctgaccgat
     5221 tccaatagga aagacttcta cagtattact gtctggcggg tagtaataaa gacgcatctc
     5281 agcgacgtta accacaatcc ctttacgaac agtggcgggc aaaatcagtt gctgcggaat
     5341 ggtgagttgc gagccagact tcggcaaaaa aacatcagcg cccgggttcg cttcaagcat
     5401 gttacttaac ccttgcccgt attgtgcggc aaaagtctcc agcggctggg tattgtgatc
     5461 aggaacagtt acagtaaacg actgccccac taaacggcta ccctctggag gtaatggata
     5521 agttaccgcc aggctagtat ggctggcaaa aagcagagca aatgagcaaa gaatatttac
     5581 acgacgcatc atgtcccttt cctaagtcgc gaaagctatc cgttaagtat agcttttatc
     5641 agacttttcg tttttaactg ttcaaatcag aagtcgtatt ccccggtaga acaatattac
     5701 tggcagcaag ttcgcccatg ttgttatata tcgcacaggc agcttcgatg atgggcatcg
     5761 ccagagctgc gccactcccc tcacctaaac gcatctccat attgagataa ggctccagcc
     5821 ccaaatgcga cagcgctata cgcgcccctt tttctgccga caggtgagaa ggaatgagat
     5881 acggtttgat tgcaggagac atctggcagg ctgcgagcgc agcagcataa gaaagaaatc
     5941 catccagcaa cacgggtaaa ccgcaggaag cagcgcctaa catcacgcca gccattccga
     6001 ccaaatcaaa tccacccact tttgccagaa catcaacgcc atcctgcgga tttggttgat
     6061 tcaacgtaat cgcccggcgc acaacatcaa ttttattagc cagtttatct gtcggcaggt
     6121 ttgcgccaat cccaaccact tcttcaggat cccggccagt gattgtgctg actattgctg
     6181 ctgccggtgt cgtgtttgcc atccccagtt cacctacacc aaacagcgtg acaccgtttt
     6241 ttgccagctc ccgcgtataa catatgacgt cgaaaagcaa cttttcagcc tgacggcgac
     6301 tcattgccgg agctgaagca atattgccgc tacctcgtgc gacacgcatg ttgataagtc
     6361 cggggatagg ctcagcacta tcaataccaa catcaactac gtggacgtta gcgcccgctt
     6421 gtgctgccag cacacacacg ccggttgttc cgcgggtcat attttcagcc tgaatggctg
     6481 tcacttcttt tggggaaata gcgacccctt cctcccagac gccgtgatcg gcacacataa
     6541 ccagtaccgc ttttttgccc acatgcggta tgccattcaa ccccggcatt cccgccagtt
     6601 gtatggcaag cgcctccagc cttcccaggc taccaacagg tttgagtaac ccgtcaatat
     6661 gccgttgtgc acgcgacata gcggcaggat cgatagcagg gatcgtattc agtaaatcgg
     6721 caagtgtttg catgtcacgt ctctttatag cagtgccagc aggaagacca gttcaccaag
     6781 ctctatagca gctcccagcg tatcgccggt ttgcccgccc agcgtacgtt tgagaagctg
     6841 accgaggatg aaaatcgcca ccatcgtcac caccatagca gccacaccat gcatacccgg
     6901 caataatacg gcagcaaaaa tagcggctaa gccgagtgtg acgcaggttt gtcgcccatc
     6961 aattttgcca atgaacacat tgccaagcct ttcttcccgg gcataacgat gacgatacat
     7021 caataatgcg gcagtcccgc ggctgaccgc acatgctgcc gccagcgatg caaggatcgg
     7081 ttcgccacgc agagccaact cgcttaacac cagaatcttt gccagtacaa caaaaatcaa
     7141 tgccagaccg ccgtgggtgc ctaaacggct atctcgcatg atttccaaca tgcgatcgcg
     7201 gctacgtgca gaaaatacgc catcacaggt atcagcgagg ccgtccaggt ggaatccccc
     7261 ggtcatcagc gccagcacaa gtacgctaaa cagtgccgcc agtggtacgc cacaccatgc
     7321 ctgcagcacc atgaaaacca gcccgctaat cgcgccaagc aataatccaa ttaaaggaaa
     7381 agtaataata ccgcgagaat aatgctcgaa atccagtccc tgggaccagc gacgcggcac
     7441 aggcagacgc gtaataaaag agagcatcgc ccaaaataat ttactcattt gatttttact
     7501 ccaatacccg aaaccaccag ccacacttca tttgccgcag cggccagctg ctgatttacc
     7561 cgcccggcaa tatcacgaaa atgtcgtgcc agacgactct ccggcacaat ccccattccc
     7621 acttcgttag tcactaatac tacctttgcg gggcaacgtt ggcaggcagc aatcaatgac
     7681 tgaatctcag catgaatcgc ctgttccatc gcctgataat cccattcatc agggtcttta
     7741 tcgccgccat aatcaaacaa cagattagtc accattgtgg taacgcattc cagcaacaca
     7801 gcttcatgcg ggttaatgtc tgcatgaatt aattcatcaa gatgttgcca gcgctccact
     7861 gtgtgccagt gcgccgggcg gccttgccga tgatgttcta tccgtgcagc catctcatca
     7921 tctaggattt gtgaggtggc gatatacaga acctgtgaag agtccccaat aagcgcctct
     7981 gcatggcgac tcttcccgct ccgtgcgccc cccgtcacca aaatcatcat accgactcct
     8041 gattcaattg tatgatggta ttcattgaac agaacaatgg atcccccacc gttgctgacc
     8101 gcaaaacatt tacgcgatta atactgcgcg taatataatt aaaaatccag cattctcaat
     8161 ccattacgaa agaagatggc attgtggaat agccagactt cctcgtgtta acgtccgaca
     8221 aaatgcgtca ttgacagcgg tttctctgga atgacagatt tcattcagat aatggattaa
     8281 tgctacagcc ccaccagccg gaccagtcag taaacgggca ttgtgttctg caatcaaaat
     8341 taccgtcgtt ccctcagaat gagggagcca ttctggtacg gatatctcgc tttcagctgc
     8401 tatcaatagc tgcactgaat aatcgctata cgcatctgat aatgattgca cagacccacc
     8461 gttaagaggc tggaaatctt gctgccagtc ctcacggata aaaccgcagc ctctacagtg
     8521 cattgttggt cagtgcctgc gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac
     8581 cgtggtcacc tttttcgcca ggctttacct cgtcaatgaa tcgcccggtc attggagtag
     8641 taaccaaagc agatttagcc agcatggaag aaatatcttt ggtgaaatgt tggttacggg
     8701 aagctggagc gcacaacgtg ttagtaacca gtgcggttaa taacaataga gttgcagaac
     8761 tttttgcctt gctgcataca gaagaagtct gtcgctaatt tcgataggtt atcagatggg
     8821 aatgatgact ttgatacaca gaaaagaatc aatgagcaat gagggttgcc aggcaaccct
     8881 cattgaataa aacaggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag
     8941 ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc
     9001 aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct
     9061 catccccggc ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct tctggttgtc
     9121 acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag
     9181 acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct
     9241 gataatgtga gagtcggatt cgtttaagca acagctattc gttagggcca acattactca
     9301 tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct
     9361 gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca
     9421 aatcagattc gatagcaact caagctgatg cgccgtttcc atgttcagcc acacataacc
     9481 atagattggt gtttccacta ccttcaggtc atggtggtta ttgagcaact gacgcagctc
     9541 ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta
     9601 cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt
     9661 ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa
     9721 ctccagcata caaaccagat tgcgattaat ggtctggata ccttcatata tcgattttgg
     9781 aatacgagtt tctttgctgg cgggcgcaat cagcccacgc attttcacgg catcggtcaa
     9841 gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga
     9901 ttgatagacc cgattatact cggtcagact tttcgccagt tgaatacgcc agtggatgaa
     9961 cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc
    10021 gccgcttcgc cataacgccg tatcaatttc acctgtaggg gaacccacaa caattgccag
    10081 cgtcacccca atcaataaac cttgatacgg tttcttgccc agcgccagcc aaccgcaaag
    10141 aaacatagcc gccgcgcacc agactaacat cagcggtaac gaaattaact ccagttgcag
    10201 agcgataaga cctaaaatcg aacccaacac cgtaccgcca atacgttcga aggcgcgagg
    10261 gacaacgtta ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca
    10321 ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc
    10381 gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa acgggcttaa
    10441 tgacttatcg gcacgcacgc agaaactccg taatgaagat taaaagcgct cataactcat
    10501 ctgtcggcca gaaggcgcgc ctctgaatta tccgacggac aaaccgggaa cagtgtaatt
    10561 tcccggcagg agcgaaaaaa ttgaaaggcg catcttactc ttttcgcttt caaaaaaaag
    10621 agtggtcatc gcgttaacac accgccctga gatgaattag tgatgttttg gctgcaccgc
    10681 aacatacatt tcgatatccc agtacccatc ttccgcgcca ttgttcaaat aaacctcgaa
    10741 gcagggcttt ggtaacattt cataagcact gtcctgcaag aggctattaa agaactgata
    10801 ccagggttta gcaaaatcat caccgactac acgagctacc gccaccgcat actgaccacc
    10861 tgaaatttct gtcagaatga cgccctcact gttttcggga agcgtaaagt aacccggcac
    10921 cgtcacgacg gtgtcgcagc gtaatttttc ggcgggtgtt tcatctggat tgtcgtaata
    10981 gacagcaacc cactccttcg gcacaatatt tttgctatct acccacatca tcaactgctc
    11041 aaagcctttc tttaccgtct gttcccacgg gccaacgaga tggaaacctg caacggtacg
    11101 tttatcttcc tgcttaatct cgtagttcat gacgcctcca ttaatactgt ttttatatac
    11161 agtatagttg caaaattaaa accacaagga atgagtgttg attatgcgag cagactcgca
    11221 ctcctgccag tctgctgcaa aagaaaggtc aggccttatg gtggaaataa tcactcaggc
    11281 gagaaaacat gccgccttcc ccgacagatt ccagggtaac cagcggccag tgcgccacct
    11341 gtttatcacg gtcgtaaagt tcaatttccc ctacccgctg atgggcgcta attggcgcgg
    11401 tgagttcttt accatcaagg gtatatttgg ctttgatatg tggaatttcg gctttcggta
    11461 gcaccatcca gaactcttgt tccgttccca ggtcgatatt ttctttatcg ccataccaga
    11521 tgcgttccgt tccgaccttt ttcccacggt gcaaaatttg caccgtagta aagttttgtt
    11581 gcccccaacg cagtaatttt cttgcctctt cctcacgacc ttttgcactg tcagccccca
    11641 ttaccactgc aatgagacga cgctgcccat ctacagccga agcaatgaga ttaaacccgg
    11701 caccagaagt atgacccgtt ttcaggccgt caacattcat ggttttatcc cacaacaacc
    11761 cgttacggtt ttgctgggtg ataccgttcc aggtgagact tttctcactg tacatatgat
    11821 aaaactcggg ctcgccgtgg atgatagcgc gagaaagcac agctaaatca taagccgagc
    11881 tatgctgacc tggcgcatcc aggccatgca ctgtttcaaa atgcgtatcc ttgagatgca
    11941 gcttctcggc atagttgttc atcatttcaa caaactgccg ttgcccaccg gcaatatagt
    12001 cagccagtgc aacacaagcg tcatttccgg aatctacaat taaaccacgg cttaaatcac
    12061 gtaccgatac gcgatcgccc tctttcaaaa acatcagtga agaaccgaca aacaccggat
    12121 tatctttcgc ccacgcatcg cgccccacgg tgacaatttc gtctggcgta atgcgatgac
    12181 tatcgatagc gcgatccacg acataacccg tcatcagctt tgtcaggctg gcgggattgc
    12241 gctgttgatg ctcattaccc gcggtgagga tctgaccggt ggtgtaatcc atcaataccc
    12301 aggacccggc atgaatctca ggaggctgag gtgaaaaagg aatgttttcc gccgcaaaac
    12361 cagacgataa ggtaaaaacg aacaaagaag cagcaataat aagacggcgt ttcaacagca
    12421 aaccctcagg agtttcaaat agctgttctt tttacggaaa tacttatgaa ctggctggaa
    12481 taaagtgcaa gaaaatgtga ctaccctctc attttcatct gacatgatct gttgccactc
    12541 gctgccaaat tgtggcgcta aagctgatta gtacggtgat atttgatact ctggcagaca
    12601 gcagaaataa cggatttaac ctaatgatga atgacggtaa gcaacaatct acctttttgt
    12661 ttcacgatta cgaaaccttt ggcacgcacc ccgcgttaga tcgccctgca cagttcgcag
    12721 ccattcgcac cgatagcgaa ttcaatgtca tcggcgaacc cgaagtcttt tactgcaagc
    12781 ctgcggatga ctatttaccc cagcctggag ccgtattaat taccggtatt accccgcagg
    12841 aagcacgggc gaaaggagaa aacgaagccg cgtttgccgc ccgtattcac tcgcttttta
    12901 ccgtaccgaa gacctgtatt ctgggctaca acaatgtgcg tttcgacgac gaagtcacac
    12961 gcaacatttt ttatcgtaat ttctacgatc cttacgcctg gagctggcag catgataact
    13021 cgcgctggga tttactggat gttatgcgtg cctgttatgc cctgcgcccg gaaggaataa
    13081 actggcctga aaataatgac ggtctaccga gctttcgcct tgagcattta accaaagcta
    13141 atggtattga acatagcaac gcccacgatg cgatggctga tgtgtacgcc actattgcga
    13201 tggcaaagct ggtaaaaacg cgtcagccac gcctgtttga ttatctcttt acccatcgta
    13261 ataaacacaa actgatggcg ttgattgatg ttccgcagat gaaacccctg gttcacgttt
    13321 ccggaatgtt tggggcatgg cgcggcaata ccagctgggt ggcaccgctg gcgtggcatc
    13381 ctgaaaatcg caatgccgta attatggtgg atttggcagg agacatttcg ccattactgg
    13441 aactggatag cgacacattg cgcgagcgtt tatataccgc aaaaaccgat cttggcgata
    13501 acgccgccgt tccggttaag ctggtgcata tcaataaatg tccggtgctg gcccaggcga
    13561 atacgctacg cccggaagat gccgaccgac tgggaattaa tcgtcagcat tgcctcgata
    13621 acctgaaaat tctgcgtgaa aatccgcaag tgcgcgaaaa agtggtggcg atattcgcgg
    13681 aagccgaacc gtttacgcct tcagataacg tggatgcaca gctttataac ggctttttca
    13741 gtgacgccga tcgtgcagca atgaaaattg tgctggaaac cgagccgcgt aatttaccgg
    13801 cactggatat cacttttgtc gataaacgga ttgaaaagct gttgttcaat tatcgggcgc
    13861 gcaacttccc ggggtcgctg gattatgccg agcagcaacg ctggctggag caccgccgcc
    13921 aggtattcac acccgagttt ttacaaggct atgcagaaga gattcagatg ctggcgcagc
    13981 agtatgccga cgataaagaa aaagtggcgc tgttaaaagc gctttggcag tacgcggaag
    14041 agatcgtcta gtttgaagcc tccgccgctg atacggcgga ggacaatgtt gggtcaggct
    14101 ttttgaacgg tgatgctcca cgcagcatcg ccaatctgct gataatcggt gatggcatgt
    14161 ccttcttctg ccgcccactg tgggattgct tcagttgcct gggtgcaatc aaactcaatt
    14221 accaactcat cgccgctggc catttctgcc agtgctgctt ttgcttctat gagtggaaac
    14281 ggacagacct gcgtcaccac atccagcttt ttaatcgcca tctttacacc ttacttaatt
    14341 tgccgcagca gttgccagtc gcgccttacg ctgcggtcga acatacacaa gccaggacgc
    14401 agtccagact ccgagaatca taaatacaag gccaacccag ccctgccagg tcatcattgc
    14461 agtcataacc agtccattac cgatagagca accacctgca atactggcac cgaagcccat
    14521 cagtacacca cctaacccgc tacgtaatgt tgtttgtgca tcagctgcgc gaacgcggaa
    14581 ctcacggctc gctttggctg caataaatga ccccacgaag atccctaaca ccaggaaaac
    14641 gccccagtta atgtatttca cgtcacctgc gaccagaaat tgcagaatat tggccgttgg
    14701 ggaagtgatt cctaacccaa acatgcgtcc ggttgcttca ctcaggggcc aggctaaaag
    14761 cgcaatcaaa ccgatgagta cagcagtgac aaagggatgc cagcgttttt caaacagaat
    14821 atgagcgatc ccggttcggc gcggcggtaa ggtcgcgact ttcagttttg gcttcttcaa
    14881 ctctttcatc accacccaga gcgttatcac cagcaaaacg gtaaccaacg gccacacaga
    14941 caaattaaaa gtctcagcaa tagagttatg ttcagtactg tagtgctgca aggtttgatt
    15001 taaaccactg gcatgtggag aacgcatcac cgcactcatc accatataag tgaaaagcgc
    15061 gatccaactg ccgatcaatc cctcacccgc gcgataccag gtcccggtgg cacatccacc
    15121 cgccagaaca attcccagcc cgaagatata cccacctata acagtaccaa gccacgggaa
    15181 cgccccggct tcgtaagtca gtaggcccgc ctgaattaac gcaaaaaccc ccacgctttg
    15241 taccgaaatt gcaatcagca aggcgtaaaa catgcgatta tttttcgcga tatacatatc
    15301 gcgaaaacca ccagtcaggc aaaaacgccc acgctgcatg acaaatccca gcagagcacc
    15361 acaaattagc ccgcttaata tcattgaaaa cataaattaa ttaaccagat gaatgttaat
    15421 ggggaaatta ttcatgactg gtggaatgca gaccaataac caaattctct aaattagaac
    15481 aaatggttct taatgaggat ttcgataaaa aagaataaaa aaaccggagc caaatattca
    15541 ttcgactccg gttgattatt agaaaagatg attatgctac gtcttcgtac tgcggaaccg
    15601 ggttacggaa gctcttggtg acgcaagcca ggtaaatcag accgatagca gcccagatca
    15661 gacccagaac cattgagctt tcttccaggt taacccacag cgcaccaacg gtcagcgcac
    15721 cacacatcgg caggaacaga tactggaagt gatctttcag cgtcttgtta cgcttctcac
    15781 ggatccagaa ctgcgagatg accgacaggt taacaaaggt gaatgccacc agcgcaccaa
    15841 agttaatcag cgccgttgcc attaccaggt cgaagttgat tgccagcagg gcaatcgcgc
    15901 caaccaggat gatgttcatc gccggagtac gccatttcgg gtgtacataa ccgaagaagc
    15961 ttttcgggaa cacgccgtca cgacccatta cgtacatcag acgcgcaaca cctgcgtgcg
    16021 ccgccatacc ggacgccagt acggtaatgg tggagaagat cagcgcgcca acctggaacg
    16081 ctttacccgc aacgtacagc atgatttcag gctgtgacgc atccggatct ttaaagcgag
    16141 agatatccgg gaagtacagc tgcaggaagt aagttgcaaa gatgaagatc atgccgccaa
    16201 tcagcgcggt caggaaaatc gcacgcggga tcacgcgctc tgcatctttg gtttcttccg
    16261 acaggttgct gatgccgtca aagccggtaa aggagaagca caggattgtc gccccggtaa
    16321 tcatcgggat aacatgcgca tcgccagacc agaacggacg agtgctcgcc agcgtaccag
    16381 cgccttcacc ttcaaatacg ccataaacaa ccatgcccag aatcaccgca atcagcacta
    16441 cctgcaacac gacgattaca gtgttgaagt tcgctacgga tttcagacta cgcaggttaa
    16501 aggcggtcat aaaggccacc agcgccacca caaacatcca cgatgggatg gaaggcacca
    16561 gagcttcaaa atagattttc gccagcagaa tgttgatcat cggcgcgaac agatagtcga
    16621 gcagagaaga ccaacccacc ataaagccga cagtcgggct aatggatttc tgggcgtaag
    16681 tatatgcaga gccagcagaa ggatagcggc gaaccagctt cccgtagctc agagccgtaa
    16741 acaggatcgc aatcaatgcg aacgcatagg ctgtcggcac atgaccatcc gtgaggcctg
    16801 aaacgatacc aaaagtatca aacagcgtca tcggctgcat ataggcaaga cccatcatta
    16861 caaccggaac taacgtaagc gttttacgca attccacgcg agaggtgttt ggagtaacgt
    16921 tatgcgacat ggtcattctc ctttacggtg ataaccgtcg cgtaagcaaa aaattgcccc
    16981 atttttttgg attcctcagc gacaacaact gtcgattttt agtaaatatc tatccggtac
    17041 gaagcccggc ctcttggtat gaatgattgg tttgaagcaa aaaataaccg acgctgatga
    17101 aacgtcggtt tttagtcatt ttttgacagc ggcgcattgt gcctaaatgg gggggaaatg
    17161 acaagagaat gagaggcttg tcataataat ttttctttaa atggctgatt ttccgtcatc
    17221 agattttgca taaacaccgg cgatcgcact atttgctaaa atttcatccc gccaccagcc
    17281 actggcaagt cctgcggtct gttcattcca gccaatccag acgggttcaa agagagtttg
    17341 ggcgaccact ttcttctcga ttaacgcgcc actatcgaga aaacgttgcg ccagataacg
    17401 gggtaaatag ccgcagccga ggccgctaat ttgcagctcc agcttggttt taaaatcgaa
    17461 aacggtaatg gcttcttgtt cgtcgagaag ttgtgaggcc gtagaagcgc ctgcctgcgc
    17521 agtgtccccc accacaatcg cccgatatcg cttaatgata cgacggttta acggctcttc
    17581 ttcctgggct aacggatgat gaggcgcgac cgcaaagact tgttccagat cgcccaaccg
    17641 cgagaaacca aactcactgg aagatggtgg ctcatgcatc gcgccaacaa taatatccgc
    17701 tcgcccctga gtcagggcat cccaggaacc accgagtacg ccattgataa acttcaggcg
    17761 cgtcacgcta tgatgttgat aaaaagcttc aatcagtggc gcaagcagag aaaaaggaaa
    17821 cgtgtcgtcc acgccaatca ccagctcgtt ttcccagcct tcatgcagtt tgatcgcctg
    17881 cttttccagt tctcgcacgg tatgcagaac ttcccgcccc ttctctaata acatttttcc
    17941 ggtacgggtg aatttagcgc ggtgaccgct acgatcaagc aattgaatat taaggtcgct
    18001 ttccagcttg tgaacggtgt aactcagagc ggacggcgtc ttgtagagtt tggccgacgc
    18061 cgccgcaaaa ctgccttctt tttctaaggc atcgaggatc atcaacacat ccagcagtgg
    18121 tttcatacac gcccccttgc agtgcatgcg gcgtcctgct ggcgtgatcg tttactccag
    18181 cggcattacc agcggatcgg gatactggta ttcaaatccc agttcattac aaatccgact
    18241 gccatcaata atcttacctt tgccgctgtc cagactattt ctgaactgcg gaggttccag
    18301 ccccagtaaa cgggccatct gcggatagaa aacattacgc gcagggtgag ctggcgcaca
    18361 tatattatag atgtgtccgc ctttaggtgc ctgtaacaac agagtgatag cgccaataac
    18421 atcttctaaa tggactaaat taacaccatg ttcaccatca ggcgcggttt ttccggcaaa
    18481 gaagcgtccg ggatgacgtc ccggtccgac caggcccgca agacgcagaa tatcgaccga
    18541 agtaccgggt aaattgtgca gccagtcttc aagttcttct aatactcgtc cactgttggt
    18601 taccggatta cgcggggtgg tttctttcac cgtgccttgc gcgtcgccat agacagacgt
    18661 tgagctggta aaaataattc ggggaatacg atgggccagc gcgctatcca ctaactcttg
    18721 taccgcttgt aaatagaact catcgccggg gccgctacga cgtgccggaa gcgtaatgac
    18781 cagcgcatcg gcatccatca gggcatccag atcgtcagaa tcgcaaacta actcaggctc
    18841 catgcgaagc agatagctat caatgccact cattcgggcc gcttcgacac catcttgtgt
    18901 ggttttactc ccggtgactt gccagcctcg cgctgaaagt gacatcgcca gcggcatgcc
    18961 taaccaccct aacccgacaa ttgcgacctt tttcatccgt tatctcctga tacaccttag
    19021 atctataagg ctacgctagc gtatcaaaac tgacaattca ttctatgaat gaaatcgaaa
    19081 cggttagcaa atataccatt gcctgatgcg ctccgcttat cagtcctaca aaaacttcaa
    19141 tatattgaat ttacgcaaca tttgtagacc agataaggca ttcacgccgc atccggcagg
    19201 aacaaagccc actttagtta cagaacacaa tgttcttgag ggttttaaag acatattttc
    19261 acgattactt ttttaacgta gtcactccag gcaccctcct ttccccgctc attcattaaa
    19321 caaatccatc gccataaaat atataaaaaa gcccttgctt tctaacgtga aagtggttta
    19381 ggttaaaaga catcagttga ataaacattc acagagactt ttatgacacg cgttcaattt
    19441 aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt gctggaagac
    19501 attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat caccttccgg
    19561 gggctttttt attgcgcggt tgataacggt tcagacaggt ttaaagagga ataagaaaat
    19621 gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa gtgatgactc
    19681 acgcgaattg ctggcgcgct gtggcattaa aatcaatctt cacacccagc gcctgatcgc
    19741 gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgacgaca ttcccggtct
    19801 ggtaatggac ggcgtggtag atcttgggat tattggcgaa aacgtgctgg aagaagagct
    19861 gcttaaccgc cgcgcccagg gtgaagatcc acgctacttt accctgcgtc gtctggattt
    19921 cggcggctgc cgtctttcgc tggcaacgcc ggttgatgaa gcctgggacg gcccgctctc
    19981 cttaaacggt aaacgtatcg ccacctctta tcctcacttg ctcaagcgtt atctcgacca
    20041 gaaaggcatc acttttaaat cctgcttact gaacggttct gttgaagtcg ccccgcgtgc
    20101 cggactggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg aagcaaacgg
    20161 cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc ctgatacagc gcgatggcga
    20221 aatggaagaa tctaaacagc aactgatcga caaactgctg acccgtattc agggtgttat
    20281 ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgcc tggatgaagt
    20341 catcgccctg ctgccaggtg ccgaacgccc aactattctg ccgctggcgg gtgaccaaca
    20401 gcgcgtagcg atgcacatgg tcagtagcga aaccctgttc tgggaaacca tggaaaaact
    20461 gaaagcgctg ggtgccagtt caattctggt cctgccgatt gagaagatga tggagtgatc
    20521 gccatgagct ttaacataat cattgactgg aatagctgta ctgcggagca acaacgccag
    20581 ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcaccgt taacgatatt
    20641 ctcgataacg tgaaagcacg cggcgatgac gcactgcggg aatacagcgc gaagtttgat
    20701 aaaaccacgg ttaccgcgct gaaggtgtct gctgaggaga tcgccgccgc cagcgaacgc
    20761 cttagcgacg agctaaaaca ggcgatggcg gtggcagtaa aaaatattga aaccttccac
    20821 actgcgcaaa aactgccgcc ggtagatgta gaaacccagc caggcgtgcg ttgccagcag
    20881 gtcacgcgcc cggtagcttc agttggtttg tatattcctg gcggctccgc cccgctcttc
    20941 tcaacggtat taatgctggc aactccagcg cgaattgcag gttgtaaaaa agtggtgctg
    21001 tgctcaccgc cgccgattgc cgatgagatc ctttatgcgg cgcagctgtg cggtgtgcag
    21061 gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg tacggaatct
    21121 gtgccgaaag tggacaaaat cttcgggcca ggtaacgcct ttgtcaccga ggcaaaacgt
    21181 caggtgagcc agcgtctgga cggtgcggcg atcgatatgc ccgcaggccc gtcggaagtg
    21241 ctggtgattg ctgacagcgg cgctacgccg gatttcgtgg cttctgattt gctttcccag
    21301 gctgaacacg gcccggactc acaggtgatt ttactgacgc ctgacgctga tatggcgcgt
    21361 cgcgttgccg aagctgtcga acgccagttg gcagaactgc cgcgtgccga aaccgcccgt
    21421 caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg cgtagagatc
    21481 tccaaccagt acggcccgga gcacctgatc attcagaccc gcaacgcccg cgaactggtc
    21541 gatagcatca ccagcgccgg ttcggtattt cttggtgact ggtcaccgga atccgcaggt
    21601 gattacgcct ccggcaccaa ccacgttctg ccgacttacg gttacaccgc cacctgttcc
    21661 agcctcgggc tggcggattt ccagaagcgt atgaccgtgc aggaactgtc gaaagaaggc
    21721 ttctccgcgc tggcttcaac cattgaaaca ctggccgccg ccgagcgcct gaccgcccat
    21781 aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc aagcatgagc accgtgacta
    21841 ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg gcgcgtcgtc
    21901 tgggcggtaa cggcgacgtc tggctgaacg ccaacgaata ccccacagcc gtggagtttc
    21961 agcttactca gcaaacgctc aaccgctacc cggaatgtca gccgaaagcg atgattgaaa
    22021 attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagccgt ggcgcggacg
    22081 aaggtattga actactgatt cgcgcttttt gcgaaccggg taaagacgcc atcctctact
    22141 gcccgccaac gtacggcatg tacagcgtca gcgccgaaac gattggcgtc gagtgccgca
    22201 cagtgccgac gctggacaac tggcaactgg acttacaggg catttccgac aagctggacg
    22261 gcgtaaaagt ggtctatgtt tgcagcccca acaacccgac cggacaactg atcaatccgc
    22321 aggatttttg caccctgctg gagttaacgc gcggtaaggc gattgtggtt gccgatgaag
    22381 cctatatcga gttttgccca caggcatcgc tggctggctg gctggcggaa tatccgcacc
    22441 tggctatttt acgcacactg tcgaaagctt ttgctctggc gggccttcgt tgcggattta
    22501 cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt gattgccccc tacccgctct
    22561 cgacgccggt tgccgacatt gcggcccagg cgttaagccc gcagggaatc gtcgctatgc
    22621 gcgaacgagt gacgcaaatt attgcagaac gcgaatacct gattgccgca ctgaaagaga
    22681 tcccctgcgt agagcaggtt ttcgactccg aaaccaacta cattctggcg cgctttaaag
    22741 cctccagcgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt gatcagaata
    22801 aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa gaaagccagc
    22861 gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc tttttatcga
    22921 tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc gttttgacaa
    22981 actcgccttt gaaccgggcg tgatcccgga gctgctaaag ctgcaaaaag cgggctacaa
    23041 gctggtgatg atcactaatc aggatggtct tggaacacaa agtttcccgc aggcggattt
    23101 tgatggaccg cacaacctga tgatgcagat cttcacctcg caaggcgtac agtttgatga
    23161 agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc cgaaagtaaa
    23221 actggtggag cgttatctgg ctgagcaagc gatggatcgc gccaacagtt atgtgattgg
    23281 cgatcgcgcg accgacattc aactggcgga aaacatgggc attaatggtt tacgctacga
    23341 ccgcgaaacc ctgaactggc cgatgattgg cgaacaactc accaaacgag accgttacgc
    23401 ccatgtagtg cgcaacacca aagagacgca gattgacgtt caggtatggc tggatcgcga
    23461 aggtggcagc aagattaaca ccggcgttgg cttctttgat catatgctgg atcagatcgc
    23521 tacccacggc ggtttccgta tggaaatcaa cgtcaaaggc gacctctata tcgacgatca
    23581 ccacaccgtc gaagataccg gcctggcgct gggggaagcg ttaaaaattg ccctcggcga
    23641 taaacgcggt atttgccgct ttggttttgt gctgccgatg gacgaatgcc ttgcccgctg
    23701 cgcgctggat atctctggtc gcccgcacct ggaatataaa gctgaattta cctaccagcg
    23761 cgtgggcgat ctcagcaccg agatgatcga gcacttcttc cgttcgcttt catacaccat
    23821 gggcgtgacg ctacacctga aaaccaaagg taaaaacgat caccaccgtg tagagagcct
    23881 gttcaaagcc tttggtcgta ccctgcgcca ggccatccgc gtcgaaggcg acaccctgcc
    23941 ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg atactggctg cgccaacctg
    24001 aactctgtga agtctgccat tgcgcgccac ggttatgaac ccaaagtcag ccgtgacccg
    24061 gacgtcgtgt tgctggccga taaactgttt ttacccggcg ttggcaccgc acaagcagcg
    24121 atggaccagg tgcgtgagcg cgagctattt gatctcatca aagcctgtac ccaaccggtg
    24181 ctgggcatct gcttagggat gcaactgctc gggcgacgta gcgaagagag caatggcgtc
    24241 gacttgctgg gcatcatcga cgaagacgtg ccgaaaatga ccgactttgg tctgccactg
    24301 ccacatatgg gctggaatcg cgtttacccg caggcaggca accgcctgtt tcaggggatt
    24361 gaagacggcg cgtactttta ctttgttcac agctacgcaa tgccggtcaa tccgtggacc
    24421 atcgcccagt gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga taacttctac
    24481 ggcgtgcagt tccacccgga gcgttctggt gccgctggag ctaagttgct gaaaaacttc
    24541 ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg gtgcgtctcc
    24601 atcagggcga ttacggcaaa caacgcgatt acggtaacaa cccgctgccg cgcttacagg
    24661 attacgctgc gcaaggtgcc gaagtgttgc acctggtgga tctgaccggg gcaaaagatc
    24721 cggctaagcg tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac gttccggtgc
    24781 aggttggcgg cggcgtgcgt agcgaagaag acgtggcggc gttactggaa gcgggcgttg
    24841 cgcgcgtggt ggttggctcc accgcggtga aatcaccaga aatggtgaaa ggctggtttg
    24901 aacgttttgg tgccgatgcc ttagtgctgg cgctggatgt ccgtatcgac gagcaaggca
    24961 acaagcaggt ggcagtcagc ggctggcaag agaactcagg cgtttcgctg gaacaactgg
    25021 tggaaaccta tctgcccgtc ggcctgaaac atgtgttgtg taccgatatc tcgcgcgacg
    25081 gcacgctggc aggttctaac gtctctttat atgaagaagt gtgcgccaga tatccgcagg
    25141 tggcatttca gtcctccggc ggtattggcg acattgatga tgttgctgct ctacgtggta
    25201 ctggcgtgcg cggcgtaata gttggtcggg cattactgga aggtaaattc accgtgaagg
    25261 aggctatcgc atgctggcaa aacgcataat tccatgtctc gacgttcgtg atggtcaggt
    25321 ggtgaaaggc gtacagtttc gcaaccatga aatcattggc gatatcgtgc cgctggcaaa
    25381 acgctacgcc gaagaaggtg cagacgaact ggtgttctac gatatcaccg cttccagcga
    25441 tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg acattccgtt
    25501 ctgtgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt cctttggcgc
    25561 ggataaaatc tccatcaact cccctgcgct ggcagaccca acattaatta ctcgcctggc
    25621 ggatcgcttt ggcgtgcaat gcattgtggt cggcattgat acctggtacg acgctgaaac
    25681 aggtaaatat catgtgaatc aatataccgg cgatgaaagc cgcacccgcg tcactcagtg
    25741 ggaaacactc gactgggtac aggaagtgca aaaacgcggt gccggagaaa ttgtcctcaa
    25801 catgatgaat caggacggcg tgcgtaacgg ttacgacctc gagcagctga aaaaagtgcg
    25861 tgaagtttgc cacgtcccgc tgattgcctc cggtggcgcg ggcaccatgg aacacttcct
    25921 cgaagccttc cgcgatgccg acgttgacgg cgcgctggca gcttccgtat tccacaaaca
    25981 aataatcaat attggtgaat taaaaacgta cctggcaaca cagggcgtgg agatcaggat
    26041 atgttaacag aacaacaacg tcgcgaactg gactgggaaa aaaccgacgg actaatgccg
    26101 gtgattgtgc aacacgcggt atccggtgaa gtgttaatgc tgggctatat gaacccggaa
    26161 gccttagaca aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac taaacagcga
    26221 ctgtggacca aaggcgaaac gtcgggcaat ttcctcaacg tagtgagtat tgcgccggac
    26281 tgcgacaacg acactttatt agtgctggcg aatcccatcg gcccgacctg ccacaaaggc
    26341 accagcagct gcttcggcaa caccgctcac cagtggctgt tcctgtatca actggaacaa
    26401 ctgctcgccg agcgcaaatc tgccgacccg gaaacctcct ataccgccaa actgtatgcc
    26461 agcggcacca agcgcattgc acagaaagtg ggggaagaag gggtggaaac tgcgctggca
    26521 gcaacggtac atgaccgctt tgagctgacc aacgaggcat cggatttgat gtatcacctg
    26581 ctggtgctgt tgcaggatca ggggctggat ttaacgacgg tgattgagaa cctgcgcaaa
    26641 cggcatcagt gagttgcggg gtaagcggat gcgatgttgt tgtcgcatcc ggcaaaaaaa
    26701 acgggcaagg tgtcaccacc ctgcccgttt tctttaaaac cgaaaaaatt acttcgcgtt
    26761 gtaattacgc agagcgttac gccccagcac aatccctgcg ccaaccatgc cgcccagcag
    26821 caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag gtaacgttgg
    26881 tttcatcaca tagcggtaag catgaatatc aagatcatca acttttaagc tttcgatatc
    26941 cagcaggttt tgacgtgtct gatagtagtt tggtgagaac accaacggac gggtcgcctc
    27001 gtgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacaacgtat cttgtgtgat
    27061 atcttcgcca gtctgttgaa tttgcggttt tgtcacctgc gcctgattcg catactgcaa
    27121 cgcttcctga atctgacgga tacgcagatc tttctgctct tgcgcgacta cttcctgagt
    27181 tcttaaagag tcctgcaagt ttttcatacg cagcgcgatg ttgtctttca agtctttttc
    27241 cagctctacg tttacctggt cgtcaacttg ctggatatac ttagccagct gcttctgagc
    27301 accttcaggt gtttcaccaa cataagacac cgctaaaggt aatgactgat ttttaacggt
    27361 aggcccaatg gtcagctttt ctgcttcttc ctggttatcc agcgtttccg ctaatgctga
    27421 gaatgcagtg ctgtaacgtc cgataagcga agcctgaatg tcgaagactt tcggtacagc
    27481 cggaccgtaa atcacattca tcgcgttggt ataaccagcg atttgcccca catcaggttg
    27541 agtaacgata gctgtggaag tccacttttc tttggcaacg gccagatacc ctatagccag
    27601 ggcaatagcc acaatgacag aaatgatgat agtcatcttg ccacgccaca actgcaccag
    27661 taaatcaatc aaatcaatct gttccgggtc aaggttttgc ccagaaacat tattattttc
    27721 tactctcatc attaccctaa cggacgaaat aattttaagt cgccatagtt tagcgataat
    27781 cgccatgaat gctataggat aaatgataaa aacaatgaat tattacaaag ggaacataag
    27841 agaatcaggg cgaaaatcgc cctgataaca ggatgttagt cactgccaaa gagatcgcgg
    27901 gtgtagactt tgtctgccac atccttaagc tcttctgcca tacggttgga aataatgacg
    27961 tcggcttgtt gtttgaaggt ggcgagatct cgttccaggc gagagttgaa gaatgagtct
    28021 tctttcatca ccggttcgta aatgatcact tcaacgcctt tcgccttgat acgtttcata
    28081 atcccctgaa tggaagacgc gcggaagttg tctgaaccgc tcttcataat cagacgataa
    28141 atacccacca cttgcggctt gcgtgacaaa atggcatcgg caataaaatc tttacgcgtg
    28201 cggttagcat cgacaattgc cgaaatcagg ttattcggca cagactgata attcgccaat
    28261 aactgtttgg tatctttcgg caaacaataa ccaccataac caaacgacgg attgttgtaa
    28321 tggttgccga tacgcgggtc gagacaaaca ccttcgataa tctgacgcgt attcagaccc
    28381 aaactttctg catagctatc cagttcgtta aagtacgcca cgcgcatcgc cagataggta
    28441 ttagcgaaaa gtttaatcgc ttctgcttca gtggagtcgg taaacagggt cgggatattt
    28501 tgcttaatcg cgccttcctg taacaacgca gcaaaacgtt ctgcgcgttc tgaacgctca
    28561 ccgatgacaa tacgtgacgg atgaaggtta tcgtaaaggg ctttaccctc acggagaaat
    28621 tccggggaga atataatatt ttcagtacga tattttttat gcatcgctgc ggtaaaacca
    28681 acgggaatcg ttgacttgat caccataacc gcataaggat ttatctcaac tacgtcttta
    28741 attactgatt ctacactgga tgtattgaaa taattagttt taggatcata gtcggttgga
    28801 gtagcgatga tgacataatc agcatcccgg taggcttcat ttttatctaa tgtagcatta
    28861 aagtgtattt tatctgattg caaaaactgc tgaatttcct tatcaacaat aggagatatc
    28921 cgatcattca gcatagcaac gcgtgacggt aaaatatcta atgccacaac ctcatgattt
    28981 tgtgcgatta gaagcccgtt tgacaagcct acatagccag taccggaaat ggtgattttc
    29041 atttcatgct ctcagaatta acttaacagt gaatcatgat gtttttagca tcctgataag
    29101 agctaaaagt tttaacgcta cgttgtcaga tggtcaacgc aacgaaaaac aattcagatt
    29161 ttctttataa gaatagctga tatttattaa gttaatatta agcagtgaaa tctagtctat
    29221 aagataattg aaaaaaaaag cccgacgcaa tatacgccgg gcctcaattt attgttggtt
    29281 aaatcagatt aatccagcca ttcagtatgg aacacacctt ctttatcaat gcgcttataa
    29341 gtatgcgcac cgaaatagtc acgctgtgcc tgaatcaggt tcgcaggcag aactgcggcg
    29401 cggtagctgt cataataggc aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt
    29461 actgcataag cgacgacatc gcgcagcgcc tgctgatagt catcggcaat ttgcttgaag
    29521 tacggagcca gcagcaggtt agcgatctgc ggattttcgg cataagcatc ggtgattttc
    29581 tgcaggaact gcgcacggat gatgcaacca gcacggaaaa tcttcgcaat ttcgccgtag
    29641 ttcagatccc agttatactc ttcagacgcc gcacgcagct gagagaagcc ctgagcataa
    29701 gaaacgattt tgcccagata cagcgcacga cgaactttct cgataaactc agctttatcg
    29761 cctgctggct gcgcttgcgg accagagaga actttagacg cggcaacacg ctgatctttc
    29821 agagacgaga tataacgtgc aaacacagac tcggtaatca gcgacagcgg ttcgccgaga
    29881 tccagcgcgc tctggctggt ccatttaccg gtacctttgt tagccgcctc atccagaatc
    29941 acatcaacca ggtaattacc gtcttcatct tttttagtaa agatgtcttt ggtgatgtcg
    30001 atcaggtagc tgctaagttc accgttattc cactcggtaa aggtctgcgc cagttcttcg
    30061 ttggtgaggt tcaggccacc tttaagcagt gaataggctt cagcaatcag ctgcatatcg
    30121 ccgtattcaa taccgttgtg aaccatcttc acatagtgac cagcaccatc ggcaccaata
    30181 taggtaacac atggctcacc gtcttcagcc actgcagcga ttttgatcag gatcggcgca
    30241 accagttcat aggcttcttt ctgcccacca ggcataatgg aaggaccttt cagcgcgcct
    30301 tcttcaccac cggagacacc ggtaccaatg aagttaaagc cttcggcaga aagctcacgg
    30361 ttacgacgaa tggtgtcctg gaagaaggta ttaccaccat caatgatgat gtcaccttta
    30421 tcgaggtatg gcttgaggga atcaatagca gcatccgtgc ctgcacctgc tttcaccatt
    30481 aacaggatgc gacgaggcgt ttccagagat tcaacaaact ctttcacagt atagtaagga
    30541 accagttttt tgcctggatt ttcggcaatc acttcttccg tcttttcacg ggaacggttg
    30601 aaaatagaga cggtataacc acggctttcg atgttgagcg caaggttgcg ccccatcact
    30661 gccataccga ctacgccgat ctgttgcttt gacattgttt actcctgtca gggactctcg
    30721 aagaattcga gttggaaaga tacactcaat gaacttttat gatacatatc aaaatgatac
    30781 aataataaaa aaaagcaaac atactattat gattattgtg tgtcggaaat atcttgtagc
    30841 tttgtaacgt atctgtccac aatattttgc caatcatatt tttcagaaat aagcttagca
    30901 gcttttcttc caagcccatc gcctagcaat ggattattat gcactctagt aatggcatct
    30961 acatactcat gcaatgtgtt agctctcagg taattttcac catcagtaag tccatcaatt
    31021 ccttcaactc caacgtcgga tgatataatt ggcaatcctg ttgccatagc ttctaaaatt
    31081 ttatttttca ttcctgcgcc aagtttcaga gggcatataa aaacagtagc cattctgtaa
    31141 tacactgata catcactaac aaaaccagtc ctgatgatat tttttgaatc caataaatca
    31201 ttgggaggat ttttgccgac aatataaact tttacatcca tatacattga ctgtagcgac
    31261 ggatatattt cgttacataa aaaccttgcg gcgtccgcat tag
//
LOCUS       DAPGRN010000054        31176 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPGRN010000054 DAPGRN010000000
VERSION     DAPGRN010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.054"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..3784
                     /gene="yfaL"
                     /locus_tag="RHW33_002556"
                     /pseudo
     CDS             32..3784
                     /gene="yfaL"
                     /locus_tag="RHW33_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
     gene            complement(3925..4647)
                     /locus_tag="RHW33_002557"
     CDS             complement(3925..4647)
                     /locus_tag="RHW33_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV1646045.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4794..7421
                     /gene="gyrA"
                     /locus_tag="RHW33_002558"
     CDS             4794..7421
                     /gene="gyrA"
                     /locus_tag="RHW33_002558"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV1646046.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7570..9258
                     /locus_tag="RHW33_002559"
     CDS             7570..9258
                     /locus_tag="RHW33_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV1646047.1"
                     /translation="MSGEKKAKGWRFYGLVGFSAIALLSAGFWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLNKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9255..9878
                     /locus_tag="RHW33_002560"
     CDS             9255..9878
                     /locus_tag="RHW33_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV1646048.1"
                     /translation="MRHVLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKNNGFSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9902..14416
                     /locus_tag="RHW33_002561"
     CDS             9902..14416
                     /locus_tag="RHW33_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV1646049.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLDGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSNTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLMQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDKDGLILAGLSHAVSGKGSM
                     SHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSGKNLYMKWSMPTVYRVGDKPSAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14417..16066
                     /locus_tag="RHW33_002562"
     CDS             14417..16066
                     /locus_tag="RHW33_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV1646050.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TSVKPGVLNGRYRVTFANGNHITFVSHGETTLLTEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQAMAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16071..16847
                     /locus_tag="RHW33_002563"
     CDS             16071..16847
                     /locus_tag="RHW33_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV1646051.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIHGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGRSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(16921..18105)
                     /gene="atoB"
                     /locus_tag="RHW33_002564"
     CDS             complement(16921..18105)
                     /gene="atoB"
                     /locus_tag="RHW33_002564"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HDV1646052.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTTEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKSLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVMER
                     LN"
     gene            complement(18136..19458)
                     /locus_tag="RHW33_002565"
     CDS             complement(18136..19458)
                     /locus_tag="RHW33_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HDV1646053.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19455..20105)
                     /gene="atoA"
                     /locus_tag="RHW33_002566"
     CDS             complement(19455..20105)
                     /gene="atoA"
                     /locus_tag="RHW33_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HDV1646054.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVCAKTEARFEVAADLNTQRGDL"
     gene            complement(20105..20767)
                     /gene="atoD"
                     /locus_tag="RHW33_002567"
     CDS             complement(20105..20767)
                     /gene="atoD"
                     /locus_tag="RHW33_002567"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HDV1646055.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLNGKIWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVEIGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(20963..22348)
                     /gene="atoC"
                     /locus_tag="RHW33_002568"
     CDS             complement(20963..22348)
                     /gene="atoC"
                     /locus_tag="RHW33_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HDV1646056.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETYCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22345..24171)
                     /gene="atoS"
                     /locus_tag="RHW33_002569"
     CDS             complement(22345..24171)
                     /gene="atoS"
                     /locus_tag="RHW33_002569"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HDV1646057.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24338..27187
                     /gene="rcsC"
                     /locus_tag="RHW33_002570"
     CDS             24338..27187
                     /gene="rcsC"
                     /locus_tag="RHW33_002570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV1646058.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKADPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27387..28037)
                     /gene="rcsB"
                     /locus_tag="RHW33_002571"
     CDS             complement(27387..28037)
                     /gene="rcsB"
                     /locus_tag="RHW33_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV1646059.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28054..30726)
                     /gene="rcsD"
                     /locus_tag="RHW33_002572"
     CDS             complement(28054..30726)
                     /gene="rcsD"
                     /locus_tag="RHW33_002572"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV1646060.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7626 a         7711 c         8368 g         7471 t
ORIGIN      
        1 ccgtcttata taaaatgatg gtcagattaa tatgcggatt atctttctac gcaaggagta
       61 tttatctcta ctcccgtcaa tgattgcatc tcttttctct gctaacggtg tcgcggcggc
      121 cattgattca tgccagggat atgatatcaa agcgagttgt cacgccagca ggcaaagcct
      181 ttcaggcatt acgcaggcct ggagtatcgc cgatgggcaa tggctggttt tttcggatat
      241 gaccaataat gccagcggtg gggccgtatt tttgcaacaa ggagcggaat ttacattatc
      301 accagaaaat gaaactggaa tgactctgtt tgccaataac accgtttcag gagaatataa
      361 taacggcggg gcaatatttg ctaaagaaaa ctcaacgctg aatcttacgg atgttatttt
      421 ttctggtaac gtcgcaggcg gctatggtgg cgcaatctat tcttctggta ctaacgatac
      481 cggtgccatc gatttacgtg tcactaacgc cgtgtttcgc aataacatcg ctaatgacgg
      541 caaaggtggt gcaatttata ccatcaataa tgatatctat ttaagtgatg atgtttttaa
      601 caataaccag gcatatacat caacaagtta cagtgatggc gatggcggcg caatcgatgt
      661 cacagataat aatagcgaca gcaagcatcc ttcaggttat acgataataa ataacactgc
      721 ctttacaaat aacactgccg aaggttatgg cggggcgata tataccaata gcgcgacggc
      781 tccctatctt attgatattt ctgttgatga cagctacagc cagaacggag gcgtgttagt
      841 cgatgagaac aatagcgcag caggctatgg agatggtcct tcctctgcgg cgggtggctt
      901 tatgtatctc ggcttaagtg aagttacatt tgatattgcc gacggaaaaa cgctggttat
      961 tggcaataca gagaatgacg gagctgttga ctctattgct ggtaccgggt taatcaccaa
     1021 aacaggttcc ggcgatctgg tacttaatgc agataacaat gactttactg gtgagatgca
     1081 gattgaaaac ggtgaagtta ccctgggccg cagcaactcc ctgatgaatg tcggcgatac
     1141 gcattgccag gacgatccgc aagactgcta cggtctgacg atagggagta ttgataagta
     1201 ccagaatcag gcagagctaa atgttggctc cacccaacaa acctttgtgc actcattgac
     1261 gggctttcag aatggcactt taaatatcga tgctggtggc aatgttactg ttaatcaagg
     1321 cagttttgct ggcaccatcg aaggtgctgg tcagctcacc attgcgcaaa acggcagcta
     1381 tgtgctggcg ggggcgcagt cgatggcgct gaccggcgat atagtggtgg atgctggtgc
     1441 ggtgctttcg ctggaaggcg acgcggcaga tcttgccgct ctccaggacg atccgcagtc
     1501 gatcgtgtta aacggcggcg tgctcgatct ctctgatttc tccacctggc agagcggtac
     1561 atcatacaaa gatggccttg aagtcagtgg cagcagcgga acggttatcg gcagtcagga
     1621 tgtggtagat cttgcaggcg gaaacgatat gcatatcggc ggtgacggga aagatggcgt
     1681 ctacgtggtg gtcgatgcgg gcgacgggca ggtcagcctg gcaaatgaca atcaatacct
     1741 cggcacaacg caaattgctt ccggtacgct gatggtgagc gacaactcgc agcttggaga
     1801 tacccattat aaccgccagg ttatctttac tgataagcca caagaaagcg tgatggagat
     1861 tactgccaat gtcgatactc gctctacaac gactgagcat gggcgtgata ttgaaatgcg
     1921 cgccgacggt gaagtggcag ttgatgcggg ggtagacacg cagtggggcg cactgatggc
     1981 tgacagcagc gggcagcatc aggatgaggg tagcacattg acgaaaacgg gggcgggtac
     2041 gctggagctg accgccagcg gtacaacgca gtcggcggta cgtgtcgaag aaggcaccct
     2101 gaaaggtgat gttgctgata tccttcctta tgcttcgtca ctgtgggttg gtgatggggc
     2161 aacgttcgtt actggcgcgg atcaggatat tcagtcaatt gatgctactt ccagcggcac
     2221 tatcgacatc agcgatggta cggttttgcg cctgaccggg caggatactt ccgtcgccct
     2281 taatgcctca ctgtttaacg gcgatgggac gctggtgaat gccaccgatg gcgtgacgct
     2341 gacaggcgag cttaatacca gccttgaaac tgacagcctg acttaacttg ccgacgtgac
     2401 ggttaatggc aatctgacca atacgtccgg tgcggttagc ctgcaaaatg gcgtcgctgg
     2461 cgatacgctg acggtaaacg gtgattatac cggcggcggt acgctattgc tcgatagcga
     2521 attaaacggc gatgactcgg caagcgacca actggtattg aacggtaata ctgctggcaa
     2581 taccacggtt gtgattaatc ccattacagg gattggtgag ccgacatcta caggcattaa
     2641 agtggttgat ttcgcagctg atcccacgca gtttcaaaac aatgcgcagt tcagtctggc
     2701 aggcagtggc tacgtcaaca tgggagcgta tgactacacg ctggtggaag ataacaacga
     2761 ctggtatctg cgatcgcaag aagtaacgcc gccatcgcca cctgatcccg acccgactcc
     2821 cgatcctgat cccacgccgg atcctgatcc aacacccgac ccggaaccta cgcctgctta
     2881 ccagccggtg ttgaatgcca aagttggcgg ttatctcaat aacctgcggg cggcaaatca
     2941 ggcgtttatg atggagcgac gcgatcacgc aggtggcgat ggtcagacgc tgaatttacg
     3001 tgttatcggc ggagattatc attacacagc agcagggcaa ctggctcaac atgaagacac
     3061 ttctacggtg cagcttagcg gcgatctgtt tagcgggcgc tggggcacgg atggcgagtg
     3121 gatgcttggg attgttggtg gctacagcga taaccagggc gacagccgct cgagtatgac
     3181 cggaactcgc gccgataacc agaaccacgg ttatgccgtt gggctaacct caagctggtt
     3241 tcagcacggt aatcagaagc agggggcctg gctggataac tggctgcaat acgcgtggtt
     3301 taacaatgat gtttccgaac aagacgatgg cgcggatcat taccattcgt cggggattat
     3361 cgcctcgctg gaagccgggt atcaatggtt accggggcgt ggtgtggtga ttgaaccgca
     3421 ggcgcaggtg atttatcagg gcgtgcagca ggatgatttt accgccgcta accgtgcgcg
     3481 cgtgtcacaa tcgcagggtg atgatattca gacgcggctg ggtttacaca gcgaatggcg
     3541 taccgctgtt catgtcatac caacattaga tctgaattat tatcacgatc cccattcgac
     3601 ggaaattgaa gaggatggca gcactatcag tgacgatgcg gtgaagcaac ggggtgaaat
     3661 aaaagtggga gtcacgggca atatcagtca gcgagtgtcc ctgcgcggca gcgtggcgtg
     3721 gcagaaaggg agtgatgatt ttgcccagac ggcagggttt ttgtcgatga cggtgaaatg
     3781 gtaatcgctg tctgaccatc actattgccg gatgcggcgt aaacgcctta tctggcctac
     3841 ggagagcatt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcat tggcgcggtt
     3901 gccggatgaa tccaaacaac aacctcactt attctgcgtg tgcagcatat agttcacatc
     3961 cacgccgggg ccgagtttaa aagtattagt gatcgggttg taatgtagcc cggttatatg
     4021 tcgctcttta aggcttgtct gatctaccca gccaagcagc tctgccggtt taataaactt
     4081 cttcacatca tgcgtgcctt tgggcaccat gcgcaaaata tattccgcgc caaccaccgc
     4141 catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc cgcccggttt
     4201 caccagttgc gcacaggctc tgaccactga ctgcggatcg ggaacgtgct ccagcatctc
     4261 catacaggtc accacgtcat actgcccggc atgttttgcc gcgtgctctt ccacagtttc
     4321 ttgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agttttgcca cctgcaatgg
     4381 ctcaaagccc atatccagac cggtcaccgt cgcaccttcg cgcgccatac tctcggccag
     4441 aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc cagcacgctc
     4501 ggcaatataa cccagacgca gcgggttaat acggtgcagc ggtttgaact caccttcaag
     4561 atcccaccag cgggaggcaa cggcttcaaa tttagcgatc tcttcgtggt ctacgttatg
     4621 gtttaccggc gatttttcgg cattcattgg cacttctact ccgtaattgg caagacaaac
     4681 gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac tgcgcggctg
     4741 tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt tagatgagcg
     4801 accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc tcctatctgg
     4861 attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat ggcctgaagc
     4921 cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg aacaaagcct
     4981 ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc catggtgact
     5041 cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt tacatgctgg
     5101 tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca atgcgttata
     5161 cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa aaagagacgg
     5221 tcgatttcgt tgataactat gacggcacgg aaaaaattcc cgacgtcatg ccaaccaaaa
     5281 ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca accaacatcc
     5341 cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat gatgaagaca
     5401 ttagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg gcggcaatca
     5461 ttaacggccg tcgcggtatt gaagaagctt accgtaccgg tcgcggcaag gtatatatcc
     5521 gcgcccgtgc cgaagtggaa gttgacgcca aaaccggacg tgaaaccatt atcgtccacg
     5581 aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa ctggtaaaag
     5641 aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac ggtatgcgca
     5701 tcgtgattga agtgaaacgc gatgcggtcg gtgaagttgt gctcaacaac ctctactccc
     5761 agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat ggtcagccga
     5821 agatcatgaa cctgaaagac atcatcgcgg cgtttgttcg tcaccgccgt gaagtggtga
     5881 cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc cttgaagcat
     5941 tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat gcgccgacgc
     6001 ctgcagaagc gaaaactgcg ctggttgcta atccgtggca gctgggcaac gttgccgcga
     6061 tgctcgaacg tgcgggcgac gatgctgcgc gtccggaatg gctggagcca gagttcggcg
     6121 tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg gatctgcgtt
     6181 tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa gagctgctgg
     6241 atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg gaagtgatcc
     6301 gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact gaaatcaccg
     6361 ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgta gtagtgacgc
     6421 tctctcacca gggctacgtg aagtaccagc cgctttctga atacgaagcg cagcgtcgtg
     6481 gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac cgactgctgg
     6541 tggcgaacac tcacgatcat atcctgtgct tctccagccg tggtcgcgtc tattcgatga
     6601 aggtctatca gttgccggaa gccactcgtg gcgcgcgcgg tcgtccgatc gtcaacctgc
     6661 taccgctgga gcaggacgaa cgtatcaccg cgatcctgcc ggtgaccgag tttgaagaag
     6721 gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc ctcaccgaat
     6781 tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc gatgagctga
     6841 tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc gaaggtaaag
     6901 tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc ggtgttcgcg
     6961 gtattcgttt aggcgaaggt gataaagtcg tctctctgat cgtgcctcgt ggcgatggcg
     7021 caatcctcac cgcaacgcag aacggttatg gtaaacgtac cgcagtggcg gaatacccga
     7081 ctaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt aacggtttag
     7141 tggttggcgc ggtgcaggta gatgactgcg accagatcat gatgatcacc gatgccggta
     7201 cgctggtgcg tactcgcgtt tcggaaatca gcatcgtggg ccgtaacact cagggcgtga
     7261 tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt gctgaaccgg
     7321 ttgacgagga agatctggat accatcgacg gcagtgccgc ggaaggggac gatgaaatcg
     7381 ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc ttcatgccaa
     7441 aagggagcca tctccctttt ttggattgaa aagtccaggc tgcaaagtct gggcttttgt
     7501 cgtattaggg cgcggtaaag tttggctgtg tccgtaaaaa atggctggct atacacaagg
     7561 aatgtggcaa tgagtggtga aaaaaaggcg aaaggctggc ggttctatgg tcttgtaggt
     7621 tttagtgcaa tagcactgct ttccgctggc ttctgggcgt tgcaatatgc tggcagtggg
     7681 ccagaaaaaa cgttgtcacc gctggtggtg cataacaatc tgcaaatcga tctcaatgag
     7741 ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct tctcaccatt
     7801 ccgtttctcc acgatgttct gagtgaagat ttcgttttct attatcagaa tcatgccgat
     7861 cgtctgggca ttgaaggcag cattcgtcgc attgtctatg aacacgatct cacgctgaaa
     7921 gataagctct tttcgtcact cttagatcag cccgcgcagg cggcgctatg gcacgataaa
     7981 caaggccatc tttcacatta catggtgctg atccagcgca gtggtttaag caaactgctg
     8041 gagccattgt tatttgccgc taccagcgac agccagttaa acaaaacgga aatcagtagc
     8101 atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg caataacgtc
     8161 ctgatgttcg cgacatatca ggacaagatg ttggtgtttt ccagcacgga tatgctgttt
     8221 aaagatgacc agcaggatac ggaagccacg gcgatcgcaa gtgatttgtt gagcggcaaa
     8281 aaacgctggc aagccagctt tggcctggaa gagcgtactg ccgaaaaaac gccagtacgc
     8341 cagcgtatcg tagtcagcgc caggttgctg gggtttggct accagcgatt aatgccttct
     8401 tttgctggcg tacgcttcga aatgggtaac gacggctggc acagttttgt ggcgttaaat
     8461 gatgaatccg ccagcgtaga tgccagtttc gattttacgc cggtctggaa cagtatgcct
     8521 gccggggcca gtttctgtgt ggcggtgccg tattcacacg gtattgccga agagatgctt
     8581 tcgcacatca gccaggaaaa cgacaagttg aatggggcgt tagacggtgc cgcggggctg
     8641 tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt tgatggcact
     8701 gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc gcacgaaagc
     8761 aaagcgccag aaggtgtttt gccggtaagc cagactcagc agggcgaagc gcaaatctgg
     8821 cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc gcaacccgat
     8881 caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa aacgctgctt
     8941 ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa aacccgcccg
     9001 gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa tccacaaggc
     9061 gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct cgaaccggtt
     9121 ttttataatg ccgcacaaac tttattaatg ccgaagctgg acgctttatc tcaacaaccg
     9181 cgttatgtca tgaagctggc ccagatggaa cccggtgccg cctggcagtg gctacccata
     9241 acctggcagc cgctatgagg cacgtgctgc tggcgctgat ttgctggctg tgttgtgttg
     9301 ttgcccatag cgaaatgctg aatgtcgaac aatccgggct gtttcgcgcc tggtttgtgc
     9361 gcattgctca ggaacagctc cgccaggggc caagtccacg ctggtatcag caggattgtg
     9421 cgggcctggt gcgatttgca gcgaacgagg cgctgaaagt tcacgacagt aaatggctaa
     9481 aaaataacgg tttctctaac cagtatttgc cgccagagat gacgctaaca cctgaacagc
     9541 ggcaactggc gcaaaactgg aatcagggga acgggaaaac cggcccctac gtaaccgcga
     9601 ttaatttgat tcagtacaac agccaattta ttggccagga cataaaccag gcgctgcctg
     9661 gcgatatgat ttttttcgat cagggcgatg cccagcactt aatggtctgg atggggcgtt
     9721 acgtcatcta ccacaccgga agcgccacga aaactgacaa cggaatgcgc gcagtcagtc
     9781 tgcaacaact tatgacatgg aaggacaccc gatggatacc caacgattcc aatcccaatt
     9841 tcattggcat ttatcgttta aattttctgg cgcgatagca gcgtgcttgt ccctgtctct
     9901 tgtgggaaca gggcttgcta atgctgatga ttcgcttcct tccagtaact atgcgccgcc
     9961 cgccggggga acattctttt tgcttgctga cagcagtttt agcagcagtg aagaggcgaa
    10021 agtgcgactg gaagcgccgg ggcgtgatta tcggcgctat cagatggaag agtacggcgg
    10081 cgtggacgtt cgcctgtatc gtattcctga cccgatggca tttttgcgcc agcagaaaaa
    10141 cctgcatcgc attgtggtgc aaccgcaata tctgggcgac gggctgaaca atacgctgac
    10201 ctggctgtgg gataactggt acggcaaatc tcgccgcgtg atgcagcgta ctttctcttc
    10261 tcagtcacgg cagaatgtga ctcaggcatt acccgaatta catctcggca atgccattat
    10321 taaaccttcc cgttatgtac agaacaacca gttttccccg ctgaaaaaat atcccctggt
    10381 ggaacagttc cgttatccac tatggcaggc taaaccggtc gagccgcagc aaggggtaaa
    10441 actggacggc gcatccagca atttcatctc gccgcagccg ggtaacattt atattcctct
    10501 cggccaacaa gagccgggac tgtacctcgt tgaggcgatg gttggtgggt atcgggcgac
    10561 gacggtggtg tttgtttcca ataccgtggc gcttagcaaa gtgtcaggca aagagcttct
    10621 ggtgtggacc gcgggtaaaa aacagggtga agcgaagcca ggctcagaga tcttgtggac
    10681 tgacggtctt ggcgtgatga cccgcggtgt gaccgatgac agcggtacct tgcagttaca
    10741 acatatatcg ccagaacgtt catacattct gggtaaggat gctgaaggcg gcgtttttgt
    10801 ctccgagaac ttcttctacg aaagcgaaat ctacaacacc cgcttgtata tttttaccga
    10861 tcgcccgcta tatcgtgcag gcgatcgtgt cgatgttaaa gtgatgggcc gcgagttcca
    10921 cgatccgttg cattcatccc ccatcgtcag cgccccggcg aagctttcgg tgctggacgc
    10981 taacggcagt ctgatgcaaa ccgtcgatgt cacgctggat gcgcgcaatg gcgggcaggg
    11041 aagtttccgc ctgccagaaa atgccgttgc cggaggttat gagttacgtc ttgcttaccg
    11101 caatcaggtc tatagcagca gttttcgcgt ggcaaactac atcaagccac atttcgagat
    11161 tggtttagct ctcgacaaaa aagagttcaa aactggcgaa gcggtcagcg gcaaactgca
    11221 acttctctac ccggatggcg agccggtaaa aaatgcccgc gtgcagttaa gtttgcgcgc
    11281 tcagcaatta tcaatggtcg gtaacgattt gcgttatgcc ggacgtttcc ccgtgtcgct
    11341 ggaaggcagc gaaacggtgt ccgacgccag cggtcatgtg gcgttaaatc tccccgccgc
    11401 cgataaaccg agccgctatt tgttaaccgt ctccgccagt gacggcgcgg cgtatcgcgt
    11461 caccaccacc aaagagatcc tcattgaacg cggcctggcg cattactctt taagtaccgc
    11521 cgcacaatac agtaatagcg gcgagtcggt tgtgttccgt tatgccgcgc tggaatcttc
    11581 aaaacaggtt cctgttacgt atgaatggtt gcgtctcgaa gaccgcacga gccatagcgg
    11641 agatctaccg tcaggcggca aatcctttac cgtcaatttc gataaacctg gcaactacaa
    11701 tctgacgtta cgcgataaag acggcttaat tctcgccggg ttaagccatg cagtcagcgg
    11761 taagggcagc atgtcgcata ctggtacggt agatatcgtg gcagataaaa cgctgtacca
    11821 gcctggcgaa accgcgaaga tgctgattac cttcccggag ccaattgatg aagcattatt
    11881 gacgctggaa cgcgatcgcg ttgaacagca gtcgctgcta tcgcatccgg caaactggct
    11941 gacgttacaa cgtttaaacg atactcagta tgaagcccgt gttccagtga gcaattcctt
    12001 tgcgcctaac atcacttttt cggtgctgta tacccgtaac ggtcagtaca gttttcagaa
    12061 cgccgggatc aaagttgccg ttccccagct ggatatccgg gtgaaaacgg acaaaaccca
    12121 ttaccagcct ggtgaactgg tcaatgtcga attaacctcg tcgctgaaag gtaaacctgt
    12181 ttctgcgcag ctaacggtag gcgtggtcga tgaaatgatc tacgcgctgc aaccagaaat
    12241 cgcgccgaat atcggcaaat ttttctatcc gctggggcgt aacaatgtgc gtaccagctc
    12301 cagtctgtcg tttatcagct acgaccaggc gctctccagc gagccggttg cgcctggcgc
    12361 aactaaccgc agcgagcggc gagtaaaaat gcttgaacgt ccacggcgtg aagaggtgga
    12421 taccgcggca tggatgccgt cactcacaac cgataaacaa ggcaaagcgt atttcacgtt
    12481 cctgatgcct gattcgttaa cccgctggcg tatcaccgcg cgtgggatga acggcgacgg
    12541 gctggtcggg caagggcgtg cttatctgcg ttcgggaaaa aatctctaca tgaagtggag
    12601 tatgccaacg gtgtatcgcg tgggcgacaa accgtcggca ggactgttta tcttcagtca
    12661 gcaggataac gaaccggtgg cgctggtgac taaatttgca ggtgctgaga tgcgccagac
    12721 gctgacgctg cacaaagggg cgaattatat ttcgctggcg cagaacattc agcaatctgg
    12781 cttgttaagc gcagaactgc aacaaaatgg gcaagtgcag gacagcatta gcacaaaact
    12841 gtcttttgtg gataacagct ggcccgttga acagcagaaa aatgtcatgc tcggcggtgg
    12901 cgataacgcg ctgatgttgc ccgagcaggc gagcaatatc cggctacaaa gtagtgaaac
    12961 gccgcaggag attttccgca acaatcttga tgcgttagtc gatgaaccgt ggggtggggt
    13021 gatcaacacc ggtagccgtc tgatcccgct cagtctcgcc tggcgttcgc ttgccgatca
    13081 tcaaagtgcc gccgctaacg acattcgtca gatgattcag gataaccgtc tgcggctgat
    13141 gcaactggcg gggcccggag cgcgctttac ctggtggggt gaagatggca atggtgacgc
    13201 cttccttacg gcatgggcat ggtacgccga ctggcaggcc agccaggcgc tcggcgtaac
    13261 gcaacaaccg gaatactggc agcatatgct cgacagctac gcggagcagg cagataacat
    13321 gccgttattg catcgggcgc tggtgctggc gtgggcacag gagatgaatc tgccgtgcaa
    13381 aacgttgttg aaagggttgg atgaagctat cgcccggcgc ggaactaaaa ctgaagattt
    13441 ctctgaggaa gacccccgcg atatcaatga cagcctgatc ctcgatacac cggaatctcc
    13501 actggcagat gcggtggcaa acgtcttaac catgacgttg ctgaaaaaag cgcagttgaa
    13561 gtccacggtg atgccacagg ttcagcaata tgcgtgggat aaagcggcaa acagcaatca
    13621 gccgctggcg cacacggttg tgctgctcaa tagcgggggc gacgctaccc aggcggctgc
    13681 tattttaagt ggtttgaccg ctgagcaatc tactattgag cgcgcgctgg ccatgaactg
    13741 gctggcgaaa tatatggcga caatgcctcc agttgttttg cctgcgcctg cgggcgcatg
    13801 ggccaaacat aagttaactg gagggggcga atactggcgt tgggttggtc agggcgtgcc
    13861 ggacattctc tcttttggtg acgaattatc gccgcaaaat gtgcaggtcc gctggcgtga
    13921 accggcaaaa acggctcaac aaagtaacat tccggtgacc gttgaacgcc agttgtatcg
    13981 gcttatcccc ggtgaagaag agatgagctt tactctgcag ccggtgacca gcaatgagat
    14041 tgacagcgat gcgctgtatc tcgatgaaat tacgcttacc agcgagcagg atgcagttct
    14101 gcgctatggt caggtggaag taccgctccc gccgggagcc gacgttgagc gcacaacatg
    14161 gggcatttca gtcaataaac ccaacgccgc gaaacagcag gggcaattgc tggaaaaagc
    14221 gcgtaatgaa atgggcgaac tggcctatat ggtgccggtg aaagagctga cgggaacggt
    14281 cactttccgc catctactgc gcttctcgca aaaagggcaa ttcgttctac cacctgctcg
    14341 ctatgtgcgt tcctatgcac ctgcgcagca aagcgttgcg gcaggtagcg aatggaccgg
    14401 aatgcaggtg aaataagtga actggcgcag aatcgtctgg ctattggcgt tggttactct
    14461 gccaacgctt gcggaggaga cgccgttaca actggcactg cgtggtgcgc agcacgatca
    14521 actgtatcag ctgtcgtcat caggcgtgac caaagcctcg acattacctg actcgctcac
    14581 cacgccgtta gggagtttgt ggaaactgta tgtttacgcc tggctggagg atacccatca
    14641 accggagcag ccctaccagt gtcgcggaaa ctccccggaa gaggtttatt gttgtcaggc
    14701 tggggagagc ataacgcgcg ataccgcgct ggtacgttcc tgtgggctct attttgcgcc
    14761 gcagcggttg catattggcg cagatgtgtg ggggcaatac tggcaacagc gtcaggcacc
    14821 tgcgtggtta gcgtctctta ctacgctgaa gccagaaacc tcggtaacgg ttaaatcgct
    14881 gcttgattca ttagcgacgt tacctgcgca aaacaaagct caggaagtct tactggatgt
    14941 ggtgctggac gaggcgaaaa tcggtgtcgc atccatgctc ggtagtcggg tacgggtgaa
    15001 aacctggagc tggtttgccg atgataaaca ggaaatccgt cagggtggtt ttgccggctg
    15061 gttaaccgat ggtacaccgc tgtgggttac tggcagtggc accagcaaaa cggtactgac
    15121 ccgttatgcg acggtgctta atcgggtttt gccagtgccg acacaagttg ccagtgggca
    15181 atgtgtagag gtggagctat ttgcccgcta tccgctgaag aaaatcacag cggaaaagag
    15241 tacgacgtca gtaaaacccg gcgtacttaa tgggcgctat cgtgtgacat ttgccaatgg
    15301 taaccacatt acgtttgtca gccacggtga gacgacctta ctgaccgaaa agggcaaact
    15361 gaaattgcag tcgcatctcg atcgtgaaga gtacgttgcc agagttctgg atcgcgaggc
    15421 gaaaagtacg ccacctgagg cagcgaaagc catgaccgtg gcgatacgca cttttttaca
    15481 gcaaaacgcc aatcgggaag gcgattgcct gaccatcccc gacagcagcg ccacgcagcg
    15541 tgtttctgct tcgccagcta ccacgggggc gcgaacgatg accgcctgga cgcaggatct
    15601 catctatgcg ggcgatccgg ttcattatca cggcagtcgc gctaccgaag ggacgctttc
    15661 ctggcggcaa gccatggccc aggccgggca gggagagcgt tacgaccaga tactcgcgtt
    15721 tgcttatccc gacaacagcc ttagccgctg gggagcgcca cgctcaacct gtcagttatt
    15781 acccaaagcg aaagcctggc tggcgaaaaa aatgccgcag tggcggcgta tattacaagg
    15841 cgagacgggg tacaacgaac cagacgtgtt tgcggtctgt cgtttagtct caggtttccc
    15901 ctataccgat cggcagcaga aacggctgtt tattcgtaac ttctttacgc ttcaggatcg
    15961 gctggattta acccatgagt atctgcacct ggcctttgat ggttatccca ccgggcttga
    16021 tgagaactat atcgaaacgc tgacccgaca attattaatg gactaatgct atgcgaaaga
    16081 tttttcttcc gttgttactg gtggcgcttt cgcctgtcgc tcacagtgaa ggtgtgcagg
    16141 aagtcgagat tgacgcaccg ctctcaggct ggcatccggc agagggcgaa gatgccagtt
    16201 tttcacaatc gatcaattac ccggcgtcgt ccgtcaacat ggcagatgat caaaatatct
    16261 cagcgcagat ccacggcaaa ataaagaatt atgctgcggc gggtaaagtt cagcagggcc
    16321 ggctggtggt caacggtgcc agtatgccgc agagagttga atccgatggt tcatttgcac
    16381 gcccttatat tttcactgaa ggcagcaaca gcgtgcaggt catcagcccg gatgggcgaa
    16441 gccgacaaaa aatgcagttt tactcaacgc cgggtacagg aacgattcgt gcacgtttac
    16501 ggctggttct ctcgtgggat acggacaata ccgacctcga tcttcacgtt gtcacgcccg
    16561 atggcgaaca cgcctggtac ggtaacaccg tgctgaaaaa cagtggtgca ctggatatgg
    16621 atgtcacgac ggggtatgga cccgagattt tcgccatgcc agcgccagtt cacgggcgct
    16681 atcaggtgta tatcaactac tatggtgggc gcagcgaaac ggaattaacc accgcccaac
    16741 tgacgctgat taccgatgaa ggatcggtca atgagaaaca ggaaacattt attgtaccga
    16801 tgcgtaatgc tggcgagctg acgctggtga aaagttttga ctggtgatta atggggttac
    16861 cttcgccgtc gatgttcaag aaaacacccg ataactttcg ctatcgggtg tttttattga
    16921 ttaattcaac cgttccatca ccatcgcaat tccctggccg ccaccaatgc acagtgttgc
    16981 cagccccagc gttttatcgc gtgcctgcat ggcatgtaat agtgtgacca gaatacgagc
    17041 accactggca ccgataggat gcccgagcgc gatggccccg ccgttgacat tcactttctc
    17101 agaatcaaag cccagggatt tcccaacggc aaggaactgt gcagcaaatg cttcattagc
    17161 ctcaatgaga tcaatatccg ccagttgcag ccccgccagt tgtaacgctt tttgcgtggc
    17221 aggtactgga cccataccca tcaatgcggg gggcacgcca ccgctggcat aacttttaat
    17281 gcgagccagg ggggtaaggc ctgctgccag cgccgcagat tcttccataa tcaccagagc
    17341 ggcagcaccg tcgttaatac cagacgcgtt cccggcggtg actgttcctg ctttatcgaa
    17401 ggccgggcgc aatgcgccta acgcttcagt cgtagaatcc gctttcggga attcgtcttg
    17461 actgaagacg aaggttttct tccgagtgac aacatttacc gggacgattt cggctgtaaa
    17521 agcaccggac tcaattgcgg ctgccgcttt acgctgtgaa tgtagcgcca gttcatcctg
    17581 catttctcgg gtaattccgt actctttggc cacgttctcg gcggtaatcc ccatatgata
    17641 accatgggtg gcgcacatca gaccatcgcg caggattacg tcataaacct gtccgtctcc
    17701 aagacgataa ccagaacgtg cttttgcatc gagtaagtag ggcgctaaac tcatattttc
    17761 catacccccc gccacaatgc tttgcgcctg acctgcctga attgcctggg cggcaagcgc
    17821 cacactttta agacccgaac cacacacttt attgaccgtg aatccgcaca ccgtttctgc
    17881 cagcccgctt tttaacagtg cctgacgtgc cggattttgc cccaacccgg cttgcaacac
    17941 gttacccata atcacttcat caatgtgtag tgaatcgatt tttgcacgtt caatggcggc
    18001 tttaattact gtcgccccca ggtcgatggc gctggtggaa gcgagtgaac cgttaaaact
    18061 accgatagca gtacgtaccg cactgacgat gacacaattt ttcattttat attccttctg
    18121 tttgtagggg tgccgtcaga acagcgttaa accaatgacg aaaatgacgc cggagaagag
    18181 cagggcagtg atgcagtagc ccatgatgtc gcggacaccg agcccagcga ttgccagtgc
    18241 tggcagcgcc cagaatggtt gtgccatgtt catccattgc tcgccgtagg cgatcgccat
    18301 taccgattta ccgagatcag cgcccagcgc ctgggctgcg ggtatcacga aaggtccctg
    18361 aataacccag tgaccgccgc cagacggaac ggcgaagtta atcagtgcag aactaaaaaa
    18421 ggtcattacc gggaaggtgt ctttgttcgc aacattgatg aagaattcgg taatgagtcc
    18481 gcccagaccg gaatgctcca tcatcagttg gatcccagcg tagaagggga attgcaccag
    18541 aataccggca gtactgcgtg ctgccgcgct gatagcacgc atataagcca ttggcgtttt
    18601 atgtagcagc agacccgcaa tcataaacat caggttgacg gtattgatgg tgatgttgaa
    18661 gccatgttcg ctgaagtaca tcgccaggta agcgataccg agtgcgccga tgatcaacgt
    18721 cagaatgcgg ctttcttcca gtcgctcgga tggtggggca tctttcggta gctgcttttg
    18781 aaaatcagcc tcttccatga gtagtttcgg atcgatactc accacgtcag acggttttgg
    18841 catcatcatg cgggtgataa atggcatcac cacaatcaac gccacagtga tgaaaatgtt
    18901 aaaaccactg aacagagtat cgcccaccgg gatcagcccg gcgatatgct caaccgggtt
    18961 gcccggtgtt gcagccaaca gaggcattga gccagagaaa ccgccacccc aggtgagaaa
    19021 accaatgtag gcgcaggcaa tgagcaacgg ataatcagaa ccgggtactc ggcgggcgac
    19081 ttcacgggca aacattgcgc cgacaaccaa accaaatccc cagttgatga cacaagcgac
    19141 tgaaccgaag aaagtgacca gcatgacgcc ctgtacgggc gtctttgcgg cggaggcggc
    19201 agtacgcagc aaacttttca ccggagcaga gctggcaagg gcatgaccgg taacgatgat
    19261 aagcgccatc tgcataccaa acgccagcaa gttccagaaa ccgtcacccc acattttcac
    19321 catgctgatc ggcgtttgtg gtgttaacca aagcgcgatc acgaaagtta gcaatgtcag
    19381 caacatggca aagatcagtg gatcgggaag ccaccggctg acaaaacgcg tcataaaacg
    19441 cgatatgcga ccaatcataa atcaccccgt tgcgtattca gatcggcggc aacttcaaac
    19501 cgagcttctg ttttggcaca cacggtggct aaatcacacc cgtcggcaat ttcggtgagc
    19561 cacattttgc cgtcaataaa acgaaagaca gccagttcag taaccagcat atgcaccgca
    19621 tgttgcgcag tgagtggcat ggtgcagcgg cgcaaaattt ttgctgaacc atctttggcg
    19681 caatgttcca tggcgatgat cactttgcgc gacccggtca ccagatccat cgcgccaccc
    19741 ataccgggca ccattttccc aggcacaacc cagttcgcga ggtttgcttc ttcgtctact
    19801 tgcaaaccgc cgagcacgca ggcatcaata tgaccgccgc ggattagcgc aaatgacatg
    19861 gcgctatcaa acatggctgc accgggtaaa acaccgcacg gttgtccgcc agcgttcacc
    19921 agatctggat gcgctgtcgt gaccgggcct aaaccgagga agccgttttc cgattgcaga
    19981 gtgatatgaa taccctccgg taaataattg gcgaccattg tgggtaaacc gatccctaag
    20041 ttaacgatgt caccatcacg aagctcttgc gccacacggc gcgcaatacg ttgtttcgca
    20101 tccattattt gctctcctgt gaaacgatga tgtggtcgat aacggcacca ggggtgacaa
    20161 tatggtcagg ttgcagctcg ccgatttcga ccagttcatc tggctctacc agcgtgatat
    20221 cagccgcaag ggctatcagg gggttaaagt tgcgggcgct aagttgatag gtcaggttgc
    20281 caagtgtgtc gcaacgatga gcgcgaatta gcgccaggtc ggcgcgcagc gggcgttcga
    20341 gcagccagat tttaccgttg agtgtcagtg tctgtttgcc ttcctctacg acggtgccga
    20401 cacccgttgg ggtgagaaaa ccaccaagtc cagctccacc acagcgaatt tgctcgatta
    20461 gcgtaccttg cggcaccaga acgacgtcca tctcaccaga tatcatgcgc cgacctgttt
    20521 ccgggttggt gccgatatgt gaagcaatca ctttgcggac tcgaccattg acgatgagcg
    20581 gaccgatgcc ggtatcaaca aacgcggtat cattggctat caatgtcagg tcgcgaacac
    20641 cagattccag taatgcttca accaggcggg atggagtgcc aatccccata aatccgccca
    20701 ccatgatggt catgccgtca cgaaagaagc cggtggcgtc ttgtaatgtc atcaattttg
    20761 ttttcatttt ttatccctct tgcatacacc gttgtgggta cagtcaggga atagcaaggg
    20821 gagtgccaga atgagattat tgggtgtttt tatttaaaca attgaatgga aaaggataaa
    20881 ttagatgggg attcataagg agaataagcg gctatgcaga aaattgcgca ctgtgcaaat
    20941 ttctgcatag caagttttgg cgttatacat ccgccggatc gataccgtat tcctggagtt
    21001 tatacatcaa tgcacggcga ctgatgccca gcattaaagc agtgcgggtt cggtttcctt
    21061 cttgttgttc cagcacttcc ataatgatgc gtttttcgac gcgtttaatt tcctctttta
    21121 aattccgctc accgacaggg gctgtttttg cctcaccagc attacagact ggctgacgaa
    21181 tctgcggcgg aagatcctca gaaaaaatga tcgggcctga attcatcacg acggcgcgtt
    21241 caataacgtt ggaaagctct cgaatatttc ccggccatga ccaggcggta agcagtgaca
    21301 ttgccatcgg atcgatgtcg ataatatcgc gctgattctc actactgaat ttttgcaaaa
    21361 agtgattagc taacagggaa atatcttccc ggcgatcgcg cagaggcggc agtattaaat
    21421 gaataacgtt aaggcgataa aagagatctt cacggaaggt gccttctttt accattgcct
    21481 gcaagtcgcg gttggtggca gcaatgatgc ggatatcaac ttttatggtc tgatggccgc
    21541 caatccgttc aaattcccgt tcctgcagaa tgcgtagtaa tttggcttgc agtacgagcg
    21601 gcatttcgcc aatttcgtcg aggagcagag taccttcgtt ggcacgttca aataatccct
    21661 gacgcaaggt ttgtgcacca gtaaatgcac ctttttcatg accaaacagt tcactttcga
    21721 gcaacgattc cggcagcgcc gcgcagttga ctttaatgaa cggccccttt gcccgccgcg
    21781 aattgtagtg aatcgctctg gcaatcaact ctttcccggt gccgctttca ccgctaatca
    21841 agacgctggc ctgagaaagg gcaattttgg cggtgtcttt gcagatgtcc atcatcgccg
    21901 ggctgttggt gagaatgtgt ccccattgcc agctggtgct cagtgcctgg tgcagatgac
    21961 ggatctcttt tttcattgac tggagttgta aagcgcgctg aacgattaaa ttcaactcat
    22021 cgagatcaaa cggtttgata acatagtcga aggctccgca gcgtagcgct tcgacggcgg
    22081 tttccacttc cgcataggcc gtcatcagaa taacgggtgt ccgggtttca tggctgcgca
    22141 tctcctttag tgccttgatg ccgtccatct ctggcatgcg gatatccatc aacaccacgt
    22201 cagggtgaat atcggcaaac aggtgtaatg ctgtgcgtcc gttgttcgca caatatgttt
    22261 cgaatccttg tagtgcaaaa gcggtgctca gcatacggcg aacattatct tcatcatcca
    22321 caataaggat gcgattaata gcagtcatac agtctgattt ccctgcgggt taatcggtaa
    22381 aataagcgtg aaggttgcgc cgtagcccgg caaactggcg acgcgaatat caccctgatg
    22441 ggcattaatg atgcgttgac ttaacgccag accaagcccg gttcctgagg ctttggtggt
    22501 gaaaaaggga tcgaagatct ttttttgcag cgagagatca atgccacagc cgttgtcctc
    22561 tatcgaaatg gcctgttgtg agtcgctgta ctgccaggtt cgaatgcgaa ttttccctcg
    22621 tgcgctgata gcctggacgg cattgatcaa gatattcagt agtacctgtt tgagcagttc
    22681 acgatcggcg ttaatcgggc ttaattcatt atccagttcg cttatgaagt cgacccgcgc
    22741 ttgtacgccg gcggtttgta ccagtaccag agtttcttca accaatgcat tgagactgac
    22801 ttgttgccat tgactgtggc gtggacgtga aaattcgagc aattgctgaa taactttgtt
    22861 aattgaatcg atttctttga gtactacgga cagatattcc tgatgtatta ggtcgctggt
    22921 ttgttggcgc aagatctgta cataaccacg aatagccgtt agcggattac gtacttcatg
    22981 cgcgacgcca gccatcagct cacccagtgt ggcgaggcgt tctgcttgcg ccatgcggcg
    23041 ctgggtttct ttgcgggcag ttaaatcaga gaaaatcacc aaagcaccta tcatttcacc
    23101 gtgcgtgtta tgaatacgac tggtagtgac actgagttca atggtgcggt cacggcctgg
    23161 aaaactgatc tccagcgcca catgttcggt gccatgttcc agtgtatcca gtactggact
    23221 gtagaactga gtattgtcga acaacatgga ataaggctgc cctaccagtt catggcgttg
    23281 atagccagtg ataacttctg ctgccgggtt catggtggtt acatcaccct ggcggtcaat
    23341 ggcaatgacg ccatcggcag cgttttcaat aatcagatcg ttaagtgtcc gcgtttcacg
    23401 cagtgcctgg gcgaggttgt taacactctg actgatttgc cccatttcac cgggcaattg
    23461 tggtaatcga gtgggaatat tttgtgccag agtcgagagg ccatcggtga tgatatcaat
    23521 attggcgctc aggcgacggg agaaaaggac aatcaacagc aggcttatca gcaaaccggc
    23581 ggtgagcaca atgataatcc tcacatccat tttccaggcc tggcggcgaa tatcttcggt
    23641 taattcattg gcccagatat agccgaggat ttcaccatta cgctcaatgg ggatcattga
    23701 attcaaaata tcgccgcgca cctgcctgcc tgaataaacc aaaggggtat ttgtacgcat
    23761 gacttcgcga ccagggtgat ctgcggcaat ggtaacgccg acattattct gatatagcgc
    23821 tgaaggcgcg taggtgatta tcgcatccag cgttttgttg taataaccag cgccgatgcc
    23881 agggaaggcg tgagtgatat tttcggtaat gggggcaagt tctgcattta atgcgcggat
    23941 acgctcctca cgtggtaagt cgatgtagag atcatagcga tcgcctagtg cctgattaag
    24001 caggttgacc acggcagaaa gttttttctc tttttcagat aagactgctg aacgtccttc
    24061 cgtttctacg atataaccaa tagtaagcgt tgggacaatg accatcagga ttgccatcag
    24121 gatcatttga ttgcgtaagc ggcgtggata aatccacttc atataatgca tgctgaaatc
    24181 cactagtctt gtccggtata tgacgattat cagaggttaa ggtgatgatt tctcggcggt
    24241 gtatcatatt ccagagaaga gagaacattg cggtaacacg cttttaccgc taccttaacc
    24301 acactccatc ggtcacctga ggcggagctt cgcccctttg aaataccttg cttcttttcg
    24361 tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc tctggctgtt
    24421 gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag aatcggaaat
    24481 tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac gcacctctga
    24541 tgtgatgaaa gagctgaagt acatcgccga aaatcgctta tcggcagaaa acggtgtgct
    24601 ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc tgtttgccga
    24661 ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat tggcgtggtt
    24721 tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg tatttttaat
    24781 cggcagcgat aacctctgca tggccaattt cggtctgcgt gatatgccag tggaacgcga
    24841 taccgcgttg aaagctttgc atgaacgcat caataaatat cgaaatgcac cacaagatga
    24901 tagcggcagt aacctctact ggatcagcga aggtccgcgc cctggcgtcg ggtattttta
    24961 cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg tcgagcagac
    25021 catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta ccattcttga
    25081 tgaaaatggt cataccctga tttcgcttac cggaccagaa agcaaaatta aggccgatcc
    25141 tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg agctggtgct
    25201 gaagaaaaat ctgccaccct catcgctaag tatcgtgtat tcggtgccgg ttgataaggt
    25261 gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc ttgccggagc
    25321 tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg cggaaagcga
    25381 cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct ccgcgccagt
    25441 gggtatctgc attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg aactggcgca
    25501 tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacacaaa ttatctgtgg
    25561 gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc aaatcagctt
    25621 cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg atgtttcttc
    25681 gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac aggcgagcca
    25741 gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc tgtatggcat
    25801 tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg atcgtctggt
    25861 gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata ttctcgattt
    25921 ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac cgcgtgaagt
    25981 gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt taggcttgta
    26041 ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc gtttacagca
    26101 ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct gtatagtttt
    26161 gcatgtccgc gctgatggcg attatctctc aatccgtgtt cgcgataccg gcgtggggat
    26221 tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggtcggaa cgggcgtaca
    26281 gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga tcagcatgat
    26341 ggacggcgac atctcggtag attcagaacc gggaatgggc agccagttta ccgtgcgtat
    26401 tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggctga gtggtaaacg
    26461 ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca gtttgcagcg
    26521 cagcggcatc gtcgtaacaa catacgaagg gcaggaaccg actcccgaag atgtgttaat
    26581 cactgacgag gtagtgagta aaaaatggca gggcagagcg gtagtgacct tctgtcgtcg
    26641 acatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg tggctgcccc
    26701 gcatgagtta ccggcattgt tggcgcgtat ttatttgatc gagatggaga gcgacgatcc
    26761 tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aatgacgata tgatgattct
    26821 ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg gatcgttggg
    26881 ctatcaatgt aaaaccgcga atgatggcgt cgatgcgctt aatgtactta gcaagaatca
    26941 tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc gcttgacgca
    27001 acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta atgcgttggc
    27061 tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctctcga agccggtaac
    27121 gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga aatcgcggga
    27181 atcgtaggac ggataaggtg tttatgccgc atcaggcatt cagtgcaaat gccagatgcg
    27241 acgctgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat gggaatcgta
    27301 ggccggataa ggcgtttacg ccgcatccgg caatcggtgc aaatgccaga taagacacta
    27361 acgcgtctta tctggcctac agatgattag tctttatctg ccggacttaa ggtcacagaa
    27421 gagagataat tcagcagggc gatatcgttc tcgacaccca gcttcatcat cgcagatttc
    27481 ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc ggtcaccagg
    27541 aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg cttgtcaccg
    27601 taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt aaatttcttc
    27661 cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg tttcagcacg
    27721 atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt gttcatggtc
    27781 agaacaataa tcgacaggct tgggaaatgg cgcttgatgt acttaattaa ggtaatgcca
    27841 tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc atccagtttc
    27901 ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt cacccactca
    27961 atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc ggcaataatt
    28021 acgttcatat tgttcatgta ataggctacc ttgctacagc aagctcttga cataactgtc
    28081 aatgtcgctg atgtattttt ctattcctgg aacatccttc tcacgaatca gatgttccag
    28141 cgtttcacat aactgcttgc cgggtaccag atttagcatg gcaaatacgc ctttaagacg
    28201 atgagccgtt tgggctaacg cagcaaagtc actggttgct gcttcagtat acagcctctt
    28261 aacatcatcc ggtactgtgt ctacaaagag cgcataatag ccgctggcat ggagttgcgc
    28321 attttcatct ccgcccagag gcgattctgt cacctcttcc tgcgccagtt gcacttcaat
    28381 taattgtaag accgcttcct gcatagcgtt gctcatattg aagttgacgc acaattgacc
    28441 aggcccaatt tcccgtacgc cagactcatc atcgcttaaa agcaagccag aggcagtaag
    28501 attagacgga ttatccgtta aaaagatatc ataatcttga ctaattaatc tttcatcggg
    28561 tgtgatacag gttgcacccc aattttctaa ctggcgagtg acaatattcc gaatttctgc
    28621 cgaagtaaca tccaccatta cgcagacatc atccagtaaa cgctcttctt cctcttcaac
    28681 ttccgggtca gctgcgagca ttttgatatg cacagagtag cgtgtaccaa gcccatcccg
    28741 cgttttgatg tttaaatgac cgcccagttt acgtgccagt tgatcgctca gccagaatgc
    28801 cagcgggtcc gccttgccat agcgatcgtt ttgggtctgg ttgataaacg ggaagtgcaa
    28861 attatccatt tcatgaatgc ttacgccttc tccggtgtcc agaatgcgga acgtcaggcg
    28921 gtcttcggag gactcatcct gatcaacctc aagggtgatt tttcccaatt gcgttgaggt
    28981 cacggcatat tgcatcagta gcagcaaaat acgtcgtaag gcatcgcgat cgccgcggcg
    29041 catatcgtgt gctttcagat gattgttaat cagcagttgc agacctttac gcttgatggc
    29101 aggcaacact gaaggcacaa cttcatcaat taaatcctgc acggagaaca gcaccgtctc
    29161 acttttccag ctatcgtccg caagcatgtt cgctaactgt atttcatcga ccagccgcac
    29221 cagcacatct gcctgattcg ccagttgttt gctttccggg gcgttgagtt tagccgcgct
    29281 ctccgccagg gactgtgcag gttctttcag cgcatcgcca atgtttttca taaaggtcat
    29341 ccgcccctgc tggtttttct catacagacg ctgcgcctgc ttgagtttct tgtttaccag
    29401 cacttcgcga tcctgatcgc gaataatgaa aatttgtgtg cgcggcgcaa cctggctgcg
    29461 gaacatgcgg atctcataca gctcgttatt gatcgtcgcc tgaataatcc cctgatgctg
    29521 ttccgccatg gtggtgatgt tttgcagatt caaatgcggc agcaaatgat cggcaatttt
    29581 gttacttatg acagtgcggt tcgattcctg atcgtgaacc agcaggccga gcggcagcag
    29641 tgagactatc tcttcattga ttgcccgtaa aatgcgcaat tcgttattga ccgccgtgct
    29701 ggggacgctt tctgtactgc ggctggagaa atggcggaat gtggtatagc caaataacgc
    29761 cagcgccagc aaaccgatgt tcagcagcag tggcagcaga atgttttgca acgtatccag
    29821 cagtaaggtg ccataaggaa cctgccagac cagtcgcata tcggtagagt tgagtgccga
    29881 ggagatttca atcttcgtac tgttaaagtg gatactgacg ctatccgtcc cttctttctc
    29941 attatcattg tctcccgttg ccgtcgcgtc tggctcaagg cggaaactgt ccagcggcat
    30001 acccggtggg atcaaatcat taatcggcag atcaaaagcc acgaccgttg ccagatgtcc
    30061 cggctggttg aacgtggtac gcaaggtaaa gtaatgaccg ttctgccagg ccaggcggcg
    30121 catgttagaa aaactttcgc gttcatcgag ggcgttggcc tgttgcaaca tctctgcacg
    30181 acgtgaatca acaatgtcac tgacggtcga ttctttaaat ccggaggtga gatctttgag
    30241 gggcagggtt gagatcagca ccagactgtt atcctgacca ttcaggtaat acattgacca
    30301 cggtacattt tctgcgcccc acaatgtgtc cagataggtg gacatccgct gagtcatctc
    30361 aagcgttgag ttgtcgtgag agccaaagat taacgcttcc gttttgcgac gcggtttttc
    30421 cagatagtag acatcctgtt tcaggcgcgt ctcttgtaaa ccttcgccgg aggaggggga
    30481 ggtcgtcgcg gcaatgttgt cgtagatctg ccaggtcacg taacgccagg tatcgacgcg
    30541 cttttggata gcgtgggtaa tgtcgacaat ctggtaactt ttatctttca gccaggcgtt
    30601 aacggcgctt tgtaccatta cccccatcgt caccagtaac acaatgatca acagtaaaaa
    30661 gaagcgggta atgctccccg gtaggagtga aaagcgggtc gtggccgttg tctctttctg
    30721 acgcattcgt gtttatgacc tgttaaaact tcgcgaagtg atagggcagt ataaagggta
    30781 cagtgtgaaa ttccagactc ttacctgatt gatgattctc tattctcccg tgatatttat
    30841 catcctgcat tgggggaaat agatgtacaa gcgccatttt gctgtacaaa taacctacaa
    30901 aaagcccaac gaaaataatt gcaaataaac aaagattgct ggaaattatg ctgatgttaa
    30961 ttatttgtga aatagttaac aagcgttata gtttttctct ggtagcacag aataatgaaa
    31021 agtgtgtaaa gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa
    31081 cagacattca gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagttatt
    31141 ctagttgcga gtgaaggttt tgttttgaca ttcagt
//
LOCUS       DAPGRN010000055        30665 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPGRN010000055 DAPGRN010000000
VERSION     DAPGRN010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.055"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            256..639
                     /gene="secE"
                     /locus_tag="RHW33_002573"
     CDS             256..639
                     /gene="secE"
                     /locus_tag="RHW33_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV1646061.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            641..1186
                     /gene="nusG"
                     /locus_tag="RHW33_002574"
     CDS             641..1186
                     /gene="nusG"
                     /locus_tag="RHW33_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV1646062.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1345..1773
                     /gene="rplK"
                     /locus_tag="RHW33_002575"
     CDS             1345..1773
                     /gene="rplK"
                     /locus_tag="RHW33_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV1646063.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1777..2481
                     /gene="rplA"
                     /locus_tag="RHW33_002576"
     CDS             1777..2481
                     /gene="rplA"
                     /locus_tag="RHW33_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV1646064.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2773..3270
                     /gene="rplJ"
                     /locus_tag="RHW33_002577"
     CDS             2773..3270
                     /gene="rplJ"
                     /locus_tag="RHW33_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV1646065.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3337..3702
                     /gene="rplL"
                     /locus_tag="RHW33_002578"
     CDS             3337..3702
                     /gene="rplL"
                     /locus_tag="RHW33_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV1646066.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4022..8050
                     /gene="rpoB"
                     /locus_tag="RHW33_002579"
     CDS             4022..8050
                     /gene="rpoB"
                     /locus_tag="RHW33_002579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV1646067.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8127..12350
                     /gene="rpoC"
                     /locus_tag="RHW33_002580"
     CDS             8127..12350
                     /gene="rpoC"
                     /locus_tag="RHW33_002580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV1646068.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12563..13102
                     /locus_tag="RHW33_002581"
     CDS             12563..13102
                     /locus_tag="RHW33_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV1646069.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13512..14645)
                     /gene="thiH"
                     /locus_tag="RHW33_002582"
     CDS             complement(13512..14645)
                     /gene="thiH"
                     /locus_tag="RHW33_002582"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV1646070.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQIEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRP"
     gene            complement(14642..15412)
                     /gene="thiG"
                     /locus_tag="RHW33_002583"
     CDS             complement(14642..15412)
                     /gene="thiG"
                     /locus_tag="RHW33_002583"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV1646071.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASV"
     gene            complement(15414..15614)
                     /gene="thiS"
                     /locus_tag="RHW33_002584"
     CDS             complement(15414..15614)
                     /gene="thiS"
                     /locus_tag="RHW33_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV1646072.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINHQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15598..16353)
                     /gene="thiF"
                     /locus_tag="RHW33_002585"
     CDS             complement(15598..16353)
                     /gene="thiF"
                     /locus_tag="RHW33_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV1646073.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16346..16981)
                     /gene="thiE"
                     /locus_tag="RHW33_002586"
     CDS             complement(16346..16981)
                     /gene="thiE"
                     /locus_tag="RHW33_002586"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV1646074.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWWLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16981..18876)
                     /gene="thiC"
                     /locus_tag="RHW33_002587"
     CDS             complement(16981..18876)
                     /gene="thiC"
                     /locus_tag="RHW33_002587"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV1646075.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGTAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18957..19057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19109..19585)
                     /gene="rsd"
                     /locus_tag="RHW33_002588"
     CDS             complement(19109..19585)
                     /gene="rsd"
                     /locus_tag="RHW33_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV1646076.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19680..20453
                     /gene="nudC"
                     /locus_tag="RHW33_002589"
     CDS             19680..20453
                     /gene="nudC"
                     /locus_tag="RHW33_002589"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV1646077.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20493..21557
                     /gene="hemE"
                     /locus_tag="RHW33_002590"
     CDS             20493..21557
                     /gene="hemE"
                     /locus_tag="RHW33_002590"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV1646078.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21567..22238
                     /gene="nfi"
                     /locus_tag="RHW33_002591"
     CDS             21567..22238
                     /gene="nfi"
                     /locus_tag="RHW33_002591"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV1646079.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22281..22871
                     /locus_tag="RHW33_002592"
     CDS             22281..22871
                     /locus_tag="RHW33_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV1646080.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREASESNIGIIFQQ"
     gene            23058..23330
                     /gene="hupA"
                     /locus_tag="RHW33_002593"
     CDS             23058..23330
                     /gene="hupA"
                     /locus_tag="RHW33_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV1646081.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23343..24038
                     /locus_tag="RHW33_002594"
     CDS             23343..24038
                     /locus_tag="RHW33_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV1646082.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24040..24459)
                     /gene="zraP"
                     /locus_tag="RHW33_002595"
     CDS             complement(24040..24459)
                     /gene="zraP"
                     /locus_tag="RHW33_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV1646083.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            24697..26073
                     /gene="zraS"
                     /locus_tag="RHW33_002596"
     CDS             24697..26073
                     /gene="zraS"
                     /locus_tag="RHW33_002596"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV1646084.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEVWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26070..27395
                     /gene="zraR"
                     /locus_tag="RHW33_002597"
     CDS             26070..27395
                     /gene="zraR"
                     /locus_tag="RHW33_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV1646085.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27392..28681)
                     /gene="purD"
                     /locus_tag="RHW33_002598"
     CDS             complement(27392..28681)
                     /gene="purD"
                     /locus_tag="RHW33_002598"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV1646086.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28693..30282)
                     /gene="purH"
                     /locus_tag="RHW33_002599"
     CDS             complement(28693..30282)
                     /gene="purH"
                     /locus_tag="RHW33_002599"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV1646087.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVSIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7228 a         7958 c         8377 g         7102 t
ORIGIN      
        1 gcgttgtagc aaaagttctg agctaattgc cgataacatt tgacgcaatg cgcactaaaa
       61 gggcatcatt tgatgccctt tttgcacgct ttcgtaccag aacctggctc atcagtgatt
      121 ttctttgtca taatcattgc tgagacaggc tctgttgagg gcgtataatc cgaaaagcta
      181 atacgcgttt cgatttggtt tgcctcgcga tcgcggggtg aaaatgtttg tagaatactt
      241 ctgacaggtt ggtttatgag tgcgaatacc gaagctcaag gaagcgggcg cggcctggaa
      301 gcgatgaagt gggtcgttgt ggtggcattg ctcctggtgg cgattgtcgg caactacctt
      361 tatcgcgaca ttatgctgcc gctgcgtgcg ctggccgtag taattctgat tgctgcagcg
      421 ggtggtgtcg cgctgttaac gacaaaaggt aaagctaccg ttgcttttgc ccgtgaagcg
      481 cgtaccgaag tccgtaaggt catttggccg actcgccagg aaacattgca caccacgctg
      541 attgtggctg cggttaccgc agtaatgtca ctgatcctgt ggggactgga tggtattctg
      601 gttcgcctgg tatcctttat cactggcctg aggttctgag atgtctgaag ctcctaaaaa
      661 gcgctggtac gtcgttcagg cgttttccgg ttttgaaggc cgcgtagcaa cgtcgctgcg
      721 tgagcatatc aaattacaca acatggaaga tttgtttggt gaagtcatgg taccaaccga
      781 agaagtggtt gaaatccgtg gcggtcagcg tcgcaaaagc gaacgtaaat tcttccctgg
      841 ctacgtcctc gttcagatgg tgatgaacga cgcgagctgg cacctggtgc gcagcgtacc
      901 gcgtgtgatg ggcttcatcg gcggtacttc cgatcgtcct gcgccaatca gcgataaaga
      961 agtcgatgcg attatgaacc gcctgcagca ggttggtgat aagccgcgtc cgaaaacgct
     1021 gtttgaaccg ggtgaaatgg tccgtgttaa tgatggtccg ttcgctgatt tcaacggtgt
     1081 tgttgaagaa gtggattacg agaaatctcg tctgaaagtg tctgtttcta tcttcggtcg
     1141 tgcgaccccg gtagagctgg acttcagcca ggttgaaaaa gcctaaccca gcgatcaaaa
     1201 aagcggcgat ttaatcgttg cacaaggcgc gagattggaa tacaatttcg cgccttttgt
     1261 ttttatgggc cttgcccgta aaacgatttt ttatatcacg gggagcctct cagaggcgtt
     1321 attacccaac ttgaggaatt tataatggct aagaaagtac aagcctatgt caagctgcag
     1381 gttgcagctg gtatggctaa cccgagtccg ccagtaggtc cggctctggg tcagcagggc
     1441 gtaaacatca tggaattctg caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt
     1501 ctgccgattc cggtagtaat caccgtttac gctgaccgtt ctttcacttt cgttaccaag
     1561 actccgccgg cagcagttct gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag
     1621 ccgaacaaag acaaagtggg taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc
     1681 aaagctgccg acatgactgg tgccgacatt gaagcgatga ctcgctccat cgaaggtact
     1741 gcacgttcca tgggcctggt agtggaggac taagaaatgg ctaaactgac caagcgcatg
     1801 cgtgttatcc gcgagaaagt tgatgcaacc aaacagtacg acatcaacga agctatcgct
     1861 ctgctgaaag agctggcgac tgctaaattc gtagaaagcg tggacgtagc tgttaacctc
     1921 ggcatcgacg ctcgtaaatc tgaccagaac gtacgtggtg caactgtact gccgcacggt
     1981 actggccgtt ccgttcgcgt agccgtattt acccagggtg caaacgctga agctgctaaa
     2041 gctgcaggtg ctgaactggt aggtatggaa gatctggctg accagatcaa gaaaggcgaa
     2101 atgaactttg acgttgttat tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc
     2161 caggttctgg gtccgcgcgg cctgatgcca aacccgaaag tgggtactgt aacgccgaac
     2221 gttgctgaag cggttaaaaa cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac
     2281 ggcatcatcc acaccaccat cggtaaagtg gactttgacg ctgacaaact gaaagaaaac
     2341 ctggaagctc tgctggttgc gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac
     2401 atcaagaaag ttagcatctc caccaccatg ggtgcaggtg ttgcagttga ccaggctggc
     2461 ctgagcgctt ctgtaaacta atgcctttac gtgggcggtg attttgtcta caatcttacc
     2521 cccacgtatg ctaacgaaag ttagcagcta aaaagatttg ttcgttggag cctggcctat
     2581 ccaggcctcc gtcgaagacc gcaggagttt cgcaagaaac ttaatcccct gcgtagacgg
     2641 tgacagaacg ctaagattat tcttctatat tctggcttgt ttctgctcac cgtaattaag
     2701 acgctctctc cgtttggagg agtgaagtga gttccagaga ttttctctgg caaacatcca
     2761 ggagcaaagc taatggcttt aaatcttcaa gacaaacaag cgattgttgc tgaagtcagc
     2821 gaagtagcca aaggcgcgct gtctgcagta gttgcggatt cccgtggcgt aactgtagat
     2881 aaaatgactg aactgcgtaa agcaggtcgc gaagctggcg tatacatgcg tgttgttcgt
     2941 aacaccctgc tgcgccgtgc tgttgaaggt actccgttcg agtgcctgaa agacgcgttt
     3001 gttggtccga ccctgattgc atactctatg gaacacccgg gcgctgctgc tcgtctgttc
     3061 aaagagttcg cgaaagcgaa tgcaaaattt gaggtcaaag ccgctgcctt tgaaggtgag
     3121 ctgatcccgg cgtctcagat cgaccgcctg gcaactctgc cgacctacga agaagcaatt
     3181 gcacgcctga tggcaaccat gaaagaagct tcggctggca aactggttcg tactctggct
     3241 gctgtacgcg atgcgaaaga agctgcttaa tcgcagttat ctttttaacg cattcgctta
     3301 cgtataaact tattctgata ttcaggaaca atttaaatgt ctatcactaa agatcaaatc
     3361 attgaagcag ttgcagctat gtctgtaatg gacgttgtag aactgatctc tgcaatggaa
     3421 gaaaaattcg gtgtttccgc tgctgctgct gtagctgtag ctgctggccc ggttgaagct
     3481 gctgaagaaa aaactgaatt cgacgtaatt ctgaaagctg ctggcgctaa caaagttgct
     3541 gttatcaaag cagtacgtgg cgcaactggc ctgggtctga aagaagctaa agacctggta
     3601 gaatctgcac cggctgctct gaaagaaggc gtgagcaaag acgacgcaga agcactgaaa
     3661 aaagctctgg aagaagctgg cgctgaagtt gaagttaaat aagccaaccc ttccggttgc
     3721 agcctgagaa atcaggctga tggctggtga ctttttagtc accagccttt ttgcgctgta
     3781 aggcgccagt agcgtttcac actgtttgac tactgctgtg cctttcaatg cttgtttcta
     3841 tcgacgactt aatatactgc gacaggacgt ccgttctgtg taaatcgcaa tgaaatggtt
     3901 taagcgtgat agcaacaggc attgcggaaa gtgttccatt ttccggtcaa caaaatagtg
     3961 ttgcacaaac tgtccgctca atggacagat gggtcgactt gtcagcgagc tgaggaaccc
     4021 tatggtttac tcctataccg agaaaaaacg tattcgtaag gattttggta aacgtccaca
     4081 agttctggat gtaccttatc tcctttctat ccagcttgac tcgtttcaga aatttatcga
     4141 gcaagatcct gaagggcagt atggtctgga agctgctttc cgttccgtat tcccgattca
     4201 gagctacagc ggtaattccg agctgcaata cgtcagctac cgccttggcg aaccggtgtt
     4261 tgacgtccag gaatgtcaaa tccgtggcgt gacctattcc gcaccgctgc gcgttaaact
     4321 gcgtctggtg atctatgagc gcgaagcgcc ggaaggcacc gtaaaagaca ttaaagaaca
     4381 agaagtctac atgggcgaaa ttccgctcat gacagacaac ggtacctttg ttatcaacgg
     4441 tactgagcgt gttatcgttt cccagctgca ccgtagtccg ggcgtcttct ttgactccga
     4501 caaaggtaaa acccactctt cgggtaaagt gctgtataac gcgcgcatca tcccttaccg
     4561 tggttcctgg ctggacttcg aattcgatcc gaaggacaac ctgttcgtac gtatcgaccg
     4621 tcgccgtaaa ctgcctgcga ccatcattct gcgcgccctg aactacacca cagagcagat
     4681 cctcgacctg ttctttgaaa aagttatctt tgaaatccgt gataacaagc tgcagatgga
     4741 actggtgccg gaacgcctgc gtggtgaaac cgcatctttt gacatcgaag ctaacggtaa
     4801 agtgtacgta gaaaaaggcc gccgtatcac tgcgcgccac attcgccagc tggaaaaaga
     4861 cgacgtcaaa ctgatcgaag tcccggttga gtacatcgca ggtaaagtgg ttgctaaaga
     4921 ctatattgat gagtctaccg gcgagctgat ctgcgcagcg aacatggagc tgagcctgga
     4981 tctgctggct aagctgagcc agtctggtca caagcgtatc gaaacgctgt tcaccaacga
     5041 cctggatcac ggcccgtata tctctgaaac cttacgtgtc gacccaacta acgaccgtct
     5101 gagcgcactg gtagaaatct accgcatgat gcgccctggc gagccgccga ctcgtgaagc
     5161 agctgaaagc ctgttcgaga acctgttctt ctctgaagac cgttatgact tgtctgcggt
     5221 tggtcgtatg aagttcaacc gttctctgct gcgcgaagaa atcgaaggtt ccggtatcct
     5281 gagcaaagac gacatcattg atgttatgaa aaagctcatc gatatccgta acggtaaagg
     5341 cgaagtcgat gatatcgacc acctcggcaa ccgtcgtatc cgttccgttg gcgaaatggc
     5401 ggaaaaccag ttccgcgttg gcctggtacg tgtagagcgt gcggtgaaag agcgtctgtc
     5461 tctgggcgat ctggataccc tgatgcctca ggatatgatc aacgccaagc cgatttccgc
     5521 agcagtgaaa gagttcttcg gttccagcca gctgtctcag tttatggacc agaacaaccc
     5581 gctgtctgag attacgcaca aacgtcgtat ctccgcactc ggcccaggcg gtctgacccg
     5641 tgaacgtgca ggcttcgaag ttcgagacgt acacccgact cactacggtc gcgtatgtcc
     5701 aatcgaaacc cctgaaggtc cgaacatcgg tctgatcaac tctctgtccg tgtacgcaca
     5761 gactaacgaa tacggcttcc ttgagactcc gtatcgtaaa gtgaccgacg gtgttgtaac
     5821 tgacgaaatt cactacctgt ctgctatcga agaaggcaac tacgttatcg cccaggcgaa
     5881 ctccaacctg gatgaagaag gccacttcgt agaagacctg gtaacttgcc gtagcaaagg
     5941 cgaatccagc ttgttcagcc gcgaccaggt tgactacatg gacgtatcca cccagcaggt
     6001 ggtatccgtc ggtgcgtccc tgatcccgtt cctggaacac gatgacgcca accgtgcatt
     6061 gatgggtgcg aacatgcaac gtcaggccgt tccgactctg cgcgctgata agccgctggt
     6121 tggtactggt atggaacgtg ctgttgccgt tgactccggt gtaactgcgg tagctaaacg
     6181 tggtggtgtc gttcagtacg tggatgcttc ccgtatcgtt atcaaagtta acgaagacga
     6241 gatgtatccg ggtgaagcag gtatcgacat ctacaacctg accaaataca cccgttctaa
     6301 ccagaacacc tgtatcaacc agatgccgtg tgtgtctctg ggtgaaccgg ttgaacgtgg
     6361 cgacgtgctg gcagacggtc cgtccaccga cctcggtgaa ctggcgcttg gtcagaacat
     6421 gcgcgtagcg ttcatgccgt ggaatggtta caacttcgaa gactccatcc tcgtatccga
     6481 gcgtgttgtt caggaagacc gtttcaccac catccacatt caggaactgg cgtgtgtgtc
     6541 ccgtgacacc aagctggggc cagaagagat caccgctgac atcccgaacg tgggtgaagc
     6601 tgcgctctcc aaactggatg agtccggtat cgtttatatt ggtgcggaag tgaccggtgg
     6661 cgacattctg gttggtaagg taacgccgaa aggtgaaact cagctgaccc cagaagaaaa
     6721 actgctgcgt gcgatcttcg gtgagaaagc ctctgacgtt aaagactctt ctctgcgcgt
     6781 accaaacggt gtatccggta cggttatcga cgttcaggtc tttactcgcg atggcgtaga
     6841 aaaagacaaa cgtgcgctgg aaatcgaaga aatgcagctc aaacaggcga agaaagacct
     6901 gtctgaagaa ctgcagatcc tcgaagcggg tctgttcagc cgtatccgtg ctgtgctggt
     6961 agccggtggc gttgaagctg agaagctcga caaactgccg cgcgatcgct ggctggagct
     7021 gggcctgacc gacgaagaga aacaaaatca gctggaacag ctggctgagc agtatgacga
     7081 actgaaacat gagttcgaga agaaactcga agcgaaacgc cgcaaaatca cccagggcga
     7141 cgatctggca ccgggcgtgc tgaagattgt taaggtatat ctggcggtta aacgccgtat
     7201 ccagcctggt gacaagatgg caggtcgtca cggtaacaag ggtgtaattt ctaagatcaa
     7261 cccgatcgaa gatatgcctt acgatgaaaa cggtacgccg gtagacatcg tactgaaccc
     7321 gctgggcgta ccgtctcgta tgaacatcgg tcagatcctc gaaacccacc tgggtatggc
     7381 tgcgaaaggt atcggcgaca agatcaacgc catgctgaaa cagcagcagg aagtcgcgaa
     7441 actgcgcgaa ttcatccagc gtgcgtacga tctgggcgct gacgttcgtc agaaagttga
     7501 cctgagtacc ttcagcgatg aagaagttat gcgtctggct gaaaacctgc gcaaaggtat
     7561 gccaatcgca acgccggtgt tcgacggtgc gaaagaagca gaaattaaag agctgctgaa
     7621 acttggcgac ctgccgactt ccggtcagat ccgcctgtac gatggtcgca ctggtgaaca
     7681 gttcgagcgt ccggtaaccg ttggttacat gtacatgctg aaactgaacc acctggtcga
     7741 cgacaagatg cacgcgcgtt ccaccggttc ttacagcctg gttactcagc agccgctggg
     7801 tggtaaggca cagttcggtg gtcagcgttt cggggagatg gaagtgtggg cgctggaagc
     7861 atacggcgca gcatacaccc tgcaggaaat gctcaccgtt aagtctgatg acgtgaacgg
     7921 tcgtactaag atgtataaaa acatcgtgga cggcaaccat cagatggagc cgggcatgcc
     7981 agaatccttc aacgtattgt tgaaagagat tcgttcgctg ggtatcaaca tcgaactgga
     8041 agacgagtaa ttctcgctca aacaggtcac tgctgtcggg gtaaaacccg gcagcggatt
     8101 gtgctaactc cgacgggagc aaatccgtga aagatttatt aaagtttctg aaagcgcaga
     8161 ctaaaaccga agagtttgat gcgatcaaaa ttgctctggc ttcgccagac atgatccgtt
     8221 catggtcttt cggtgaagtt aaaaagccgg aaaccatcaa ctaccgtacg ttcaaaccag
     8281 aacgtgacgg ccttttctgc gcccgtatct ttgggccggt aaaagattac gagtgcctgt
     8341 gcggtaagta caagcgcctg aaacaccgtg gcgtcatctg tgagaagtgc ggcgttgaag
     8401 tgacccagac taaagtacgc cgtgagcgta tgggccacat cgaactggct tccccgactg
     8461 cgcacatctg gttcctgaaa tcgctgccgt cccgtatcgg cctgctgctt gatatgccgc
     8521 tgcgcgatat cgaacgcgta ctgtacttcg aatcctatgt ggttattgaa ggcggtatga
     8581 ccaacctgga acgccagcag atcctgactg aagagcagta tctggacgcg ctggaagagt
     8641 tcggtgacga attcgacgcg aaaatgggtg cggaagcaat ccaggccctg ctgaagagca
     8701 tggatctgga gcaagagtgc gaacagctgc gtgaagagct gaacgaaacc aactctgaaa
     8761 ctaagcgtaa aaagctgacc aagcgtatca aactgctgga agcgttcgtt cagtctggta
     8821 acaaaccaga gtggatgatc ctgaccgttc tgccggtact gccgccagat ctgcgtccgc
     8881 tggttccgct ggatggtggt cgtttcgcga cttctgacct gaacgatctg tatcgtcgcg
     8941 tcattaaccg taacaaccgt ctgaaacgtc tgctggatct ggctgcgccg gatatcatcg
     9001 tacgtaacga aaaacgtatg ctgcaggaag cggtagacgc cctgctggat aatggtcgtc
     9061 gcggtcgtgc gatcaccggt tctaacaagc gtcctctgaa atctttggcc gacatgatca
     9121 aaggtaaaca gggtcgtttc cgtcagaacc tgctcggtaa gcgtgttgac tactccggtc
     9181 gttctgtaat caccgtaggt ccatacctgc gtctgcatca gtgcggtctg ccgaagaaaa
     9241 tggcgctgga gctgttcaaa ccgttcatct acggcaagct tgaactgcgt ggtcttgcta
     9301 ccaccattaa agctgcgaag aaaatggttg agcgcgaaga agctgtcgtt tgggatatcc
     9361 tggacgaagt tatccgcgaa cacccggtac tgctgaaccg tgcaccgact ctgcaccgtc
     9421 tgggtatcca ggcatttgaa ccggtactga tcgaaggtaa agctatccag ctgcacccgc
     9481 tggtttgtgc ggcatataac gccgacttcg atggtgacca gatggctgtt cacgtaccgc
     9541 tgacgctgga agcccagctg gaagcgcgtg cgctgatgat gtctaccaac aacatcctgt
     9601 ccccggcgaa cggcgaacca atcatcgttc cgtctcagga cgttgtactg ggtctgtact
     9661 acatgacccg tgactgtgtt aacgccaaag gcgaaggcat ggtgctgact ggcccgaaag
     9721 aagcagaacg tctgtatcgc tctggtctgg cttctctgca tgcgcgcgtt aaagtgcgta
     9781 tcaccgagta tgaaaaagat gctaacggtg aattagtagc gaaaaccagc ctgaaagaca
     9841 cgactgttgg ccgtgccatt ctgtggatga ttgtaccgaa aggtctgcct tactccatcg
     9901 tcaaccaggc gctgggtaaa aaagcaatct ccaaaatgct gaacacctgc taccgcattc
     9961 tcggtctgaa accgaccgtt atttttgcgg accagatcat gtacaccggc tttgcatatg
    10021 cagcgcgttc tggtgcatct gttggtatcg atgacatggt catcccggag aagaaacacg
    10081 aaatcatctc cgaggcagaa gcagaagttg ctgaaattca ggagcagttc cagtctggtc
    10141 tggtaactgc gggcgaacgc tacaacaaag ttatcgatat ctgggctgcg gcgaacgatc
    10201 gtgtatccaa agcgatgatg gataacctgc aaactgaaac cgttattaac cgtgacggtc
    10261 aggaagagaa gcaggtttcc ttcaacagca tctacatgat ggccgactcc ggtgcgcgtg
    10321 gttctgcggc acagattcgt cagcttgctg gtatgcgtgg tctgatggcg aagccggatg
    10381 gctccatcat cgaaacgcca atcaccgcga acttccgtga aggtctgaac gtactccagt
    10441 acttcatctc cacccacggt gctcgtaaag gtctggcgga taccgcactg aaaactgcga
    10501 actccggtta cctgactcgt cgtctggttg acgtggcgca ggacctggtg gttaccgaag
    10561 acgattgtgg tacccatgaa ggtatcatga tgactccggt tatcgaaggt ggtgacgtta
    10621 aagagccgct gcgcgatcgc gtactgggtc gtgtaactgc tgaagacgtt ctgaagccgg
    10681 gtactgctga tatcctcgtt ccgcgcaaca cgctgctgca cgaacagtgg tgtgacctgc
    10741 tggaagagaa ctctgtcgac gcggttaaag tacgttctgt tgtatcttgt gacaccgact
    10801 ttggtgtatg tgcgcactgc tacggtcgtg acctggcgcg tggccacatc atcaacaaag
    10861 gtgaagcaat cggtgttatc gcggcacagt ccatcggtga accgggtaca cagctgacca
    10921 tgcgtacgtt ccacatcggt ggtgcggcat ctcgtgcggc tgctgaatcc agcatccagg
    10981 tgaaaaacaa aggtagcatc aagctcagca acgtgaagtc ggttgtgaac tccagcggta
    11041 aactggttat cacttcccgt aacaccgaac tgaaactgat cgacgaattc ggtcgtacca
    11101 aagaaagcta caaagtgcct tacggtgcgg tactggcgaa aggcgatggc gaacaggttg
    11161 ctggcggcga aaccgttgca aactgggacc cgcacaccat gccggttatc accgaagtaa
    11221 gcggttttgt acgctttact gacatgatcg acggccagac cattacgcgt cagaccgacg
    11281 aattgaccgg tctgtcttcg ctggtggttc tggattccgc agaacgtacc gcaggtggta
    11341 aagatctgcg tccggcactg aaaatcgttg atgctcaggg taacgacgtt ctgatcccgg
    11401 gtaccgatat gcctgcgcag tacttcctgc cgggtaaagc gattgttcag ctggaagatg
    11461 gcgtacagat cagctctggt gacaccctgg cgcgtattcc gcaggaatcc ggcggtacca
    11521 aggacatcac cggtggtctg ccgcgcgttg cggacctgtt cgaagcacgt cgtccgaaag
    11581 agccggcaat cctggctgaa atcagcggta tcgtttcctt cggtaaagaa accaaaggta
    11641 aacgtcgtct ggttatcacc ccggtagacg gtagcgatcc gtacgaagag atgattccga
    11701 aatggcgtca gctcaacgtg ttcgaaggtg aacgtgtaga acgtggtgac gtaatttccg
    11761 acggtccgga agcgccgcac gacattctgc gtctgcgtgg tgttcatgct gttactcgtt
    11821 acatcgttaa cgaagtacag gacgtatacc gtctgcaggg cgttaagatt aacgataaac
    11881 acatcgaagt tatcgttcgt cagatgctgc gtaaagctac catcgttaac gcgggcagct
    11941 ccgacttcct ggaaggcgaa caggttgaat actctcgcgt caagatcgca aaccgcgaac
    12001 tggaagcgaa cggcaaagtg ggtgcaactt actcccgcga tctgctgggt atcaccaaag
    12061 cgtctctggc aaccgagtcc ttcatctccg cggcatcgtt ccaggagacc actcgcgtgc
    12121 tgaccgaagc agccgttgcg ggcaaacgcg acgaactgcg cggcctgaaa gagaacgtta
    12181 tcgtgggtcg tctgatccct gcaggtaccg gttacgcgta ccaccaggat cgtatgcgtc
    12241 gccgtgctgc gggtgaagct ccggctgcac cgcaggtgac tgcagaagac gcatctgcca
    12301 gcctggcaga actgctgaac gcaggtctgg gcggttctga taacgagtaa tcgttaatcc
    12361 gcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag
    12421 catttgtcag aatatttaat gaatttctgg atactcataa tcaatgtaga gatgactaat
    12481 atcctgaaac tgactgaact aattgagtca agctcggcaa gaattcgata ctattcctgt
    12541 gtgactttct taaggaacga gaatgaaaca ggaagtggag aagtggcgac cttttggaca
    12601 tccggatggt gatattcgtg atttatcatt tcttgatgct catcaggctg tctacgttca
    12661 gcatcatgag ggcaaagagc ctttagagta tcgcttttgg gttacctact ctcttcactg
    12721 cttcacaaaa gattatgaac atcagacgaa cgaagaaaaa caatcgttaa tgtaccacgc
    12781 gcctaaagaa tctcgtccct tctgccagca ccgttataac ttagcgcgca cacacttaaa
    12841 aagaactatt ttggcgctgc cagaaagcaa cgttattcat gccgggtatg gtagctatgc
    12901 cgtgattgag gtggacttag acggaggaga taaggcattt tactttgttg cgttcagggc
    12961 tttcagggaa aagaaaaaac tccgtttgca tgtaactagc gcttatccca tttctgaaaa
    13021 acagaaaggt aaatcagtga aatttttcac cattgcctac aacttattga gaaataagca
    13081 gcttcctcag ccctcaaaat aacaaaaccc accttaaggt gggtttcgcc agagaattat
    13141 ctctggtatt cagaacgcca ttaccggact ttgccttgac cttgcgataa tcgcaggttg
    13201 cgggatgtct gaatttcttc agtctgctgc atcctggaag atgagaacat gtgttcttat
    13261 tttcgtctct atcatagttg agtatttact ctcttacaat cagatctctt tcattgctca
    13321 acaggcgatg gcttcagact ttgcattacg gaatttttaa gaaaggcagg gcgaaacgag
    13381 gaagaagctt ttcgccctgg acaacattcc tgctgacggt actaccataa aaaaacacaa
    13441 cgctttcaac aaccttgcgt ttttcatctt ccgtaacggc tcggaataaa tttacgttct
    13501 tacattctgt ctcatggcct ttgcgaggcg cgtcccagat agctgtccca gtctttccat
    13561 accggctgca aaccctgagc ggttaacgcg gcagcaaccg cttccggtct gcgatcgtcg
    13621 tgcggtgaga actgttccaa ctcggggtga ttatcagcat agccacctgg ctgcgttttc
    13681 gagaaggcgc tgacgttatt gatcgccagc ggaataacgc gatcgcgaaa ccacggtgat
    13741 tcccgcgtgg agagtgacag ttcaatctcc ggtgcaagca gccggaaggc gcagatggtt
    13801 tgcactaact ggcgttcatc cataatcgac gcaggctcaa tgccgccagt acatggacgc
    13861 aggcgtggga aggagacaga gtagcggctt tgccagtaat gctgttgcag ccatagcaaa
    13921 tgttctgcaa ccatatagca gtcaacgcgc cagctgtcgg aaaggccaat tagcgcgccg
    13981 aggcctatct tatcaatccc cgcacgcccc agccgatccg gcgtttccag ccgccagaag
    14041 aagtcctgtt ttttgccttt cagatgatgg cgggcataag tcgcctcgtg atatgtctcc
    14101 tgataaacca tcacgccatc cagaccaagt tgctttaact cggcgtattc cgtctccgcc
    14161 agcggctgta cttctatctg tagtgaagag aactgttcac gcagggcagg gagatgacga
    14221 cgaaagtaat ccatccccac tttcgcctga tgttcaccgg tgactaaaag cagatgttca
    14281 aagcccatct cccgtatagc ggcgctttcc ctggcaatat ccgcttcatc cagcgttttg
    14341 cgcttgatgc ggttgctcat agagaatccg cagtacgtgc agtcgttagc gcaaagattg
    14401 gaaagataaa gcgggacgta gaaactaact gtgttgccaa atcgctgacg ggtcagacgc
    14461 tgcgcccgtt gggccagttg ttccagatag ccactggcgg ctggcgataa cagcgccatc
    14521 atatcgtcgc gggtgagttg cgaggcattt agcgcccgct ctacgtcagc agccgttttg
    14581 ccgttgatac gcaggcggat gtcatcccag tccagttgtc gccagcgatc gctgaaggtt
    14641 ttcataccga tgcctccaga aatccggtca gcgggctggt ggcatgagca aaataactgc
    14701 ggctgcccgg tccggactga cgtgccagca ggcctgcttc tactgccaga cgaaatgcct
    14761 tcgccatgtt gacgggatcg tccgcgacgg caatcgccgt attcactaac accgcgtcgg
    14821 cccccatttc cagcgcctgc gcggcatggc tgggaacgcc gatgccagca tcgacaacca
    14881 ccggaactgt ggcctgctgg ataataatct ccagcatggc gcgggtttcc agcccctgat
    14941 tcgagccaat cggcgcgccg agcggcatca ccgctgcaca gccgacttct tccagacgtt
    15001 tacacaatac cggatcggcc ccgcagtaag gcagcacgac aaatccctgt tgtaccagcg
    15061 tttcggcggc tttcagggtt tcgatgggat cgggcaacag ccagcgggcg tcagggtgaa
    15121 tctccaattt tagccagttt gtgcctaacg cttcacgtgc cagatgggcg gcgaaaatgg
    15181 cttcttccgc tgttttcgcc ccggaagtat tcggcagcag ggtcacaccc gccgcgataa
    15241 gcggttcgag gatagcgtcg ttgtgctggc gcaagttgac gcgtttcatc gccagtgtca
    15301 ccagttgact gccggaagcg cggatcgcct ccaccatcag ttgtgaagag gcgaatttcc
    15361 cggtgccggt aaacagatgt gaatcaaacg ttttgtccgc aatatgtaac atctcaaccc
    15421 cctgcaataa cctgaaaaag caggatctgg tcgccatcct gcacgatatg ttgcgtccac
    15481 tgctcacgcg ggacgatttg atgattaatc gccagagccg cgcccgcttg tcgttggtcg
    15541 agttgctcca gtagttcgtg aacagtttgc ccggcggcgc actgcatcgg ttgatcgtta
    15601 aacaggatct gcattgcttc ctccgcatac cgggcaacca ctggcgcggc gcaacgccag
    15661 gctgcgccac tggctcgact taccgtcgaa cagtcggagt tctcccgcag gcgtctctat
    15721 accgcttaat aacttaatgg cttccagtgc ctgcaaagtg cccataaccc cgaccaccgg
    15781 gccaaccacg cccgccgtgc ggcagttgcg ttctggctcc tggttatctg gccacaggca
    15841 gcggtaacac ccctgctccc agggcggcgt cagtaccatc aactgaccgc caaatccgac
    15901 cgcgctggcg gtgataagcg gcgtgttgag tgccacgcag gcggcattaa tctcctggcg
    15961 agtcgccata ttgtcggtac agtcgagcac cacatcggct tgtgcaaccg catcttttaa
    16021 cgcctcaccc gttaaccgtt gttgtaatgc tgtcagttga atatcgggat tcaactgtgt
    16081 cagtcgctga tggctgacct gcgatttcgg gcgatcgata tcttcagtgg taaagaggat
    16141 ttgtcgttgc agattgctta aatgcacatc gtcgtcatct gccagtacca gcgtcccgac
    16201 gccagcgccc gccaggtata gcgcagcagg tgtacccagc ccgccaagac cgataatcag
    16261 cacctggctg tcgagcagtt tttgctgccc gtcaagagcg atatcgtcga gcaggatttg
    16321 gcggctataa cgcataaagt cacggtcatt catcgccaac tcctgcaatt tccagcaact
    16381 gtgccgttgc caaacgccag tctgcggctt gagtaatggc gctgacgacg gcgatactgc
    16441 cgacacccgt tgctatcacc gcaggcgcgc gtgccagact gataccgcca atcgccacgg
    16501 tgggataatc cgccaggcgc tcaacatgcc gtgccagctg ttccagcccc tgcggtgcag
    16561 aaggcatctg tttggtttgc gtcgggaaca catgtcccag cgcgatataa gaggggcgtg
    16621 ctgccagcgc gacgtcgatt tccatatcgt catgggtcga aacgcccagc cgcagaccag
    16681 ccgcacggat ggcgctgaga tcggtggctt gcaaatcttc ctgccctaaa tggacgccat
    16741 acgcctgatg cttgatcgcc agccaccagt aatcgttgat aaacaatcgc gcgttatagc
    16801 ggcggcccag tgcaattgcc gccacgacat cggcttccac ctcttcatcg cgccgatctt
    16861 tgatgcgtag ctggagagta cgtacgcctg catccaacag acgttcgatc cactgtacgc
    16921 tgtccaccac tgggtacagt cctaaacgaa aaggtacagg aggaaaatca ggctgataca
    16981 tcacgcttcc tccttacgca ggtagatttc tccgcctctg gcacggaagt tctccgacat
    17041 atccgccatt cccatttcaa tagtttgcgt ggcggcgtaa tcacgcactt cctggctgat
    17101 tttcatcgag cagaatttcg gcccacacat ggagcaaaaa tgggcgactt tacctgactc
    17161 ttgcggcagg gtttcatcgt gataagcgcg ggcggtaaac gggtcgaggg ccagattaaa
    17221 ctggtcttcc cagcgaaatt cgaagcgggc tttcgacatg gcgttatcgc gaatttgcgc
    17281 gcccggatgc cctttcgcca ggtcagcggc gtgggcggca atcttatagg tgataagccc
    17341 ctgcttaaca tcttctttat taggcagacc cagatgctct tttggcgtta cgtaacagag
    17401 catcgcgcag ccaaaccagc caatcatcgc cgcaccaatc cccgacgtga agtggtcata
    17461 gcccggcgca atatcggtag ttagcggccc cagagtgtaa aacggcgctt cgtggcagtg
    17521 ctctaactcc tcggtcatat tgcggcggat catctgcatc ggcacgtggc ctgggccttc
    17581 aatcatcacc tgcacatcat attcccaggc gatttttgtc agttcgccca gcgtatgcag
    17641 ctcggcaaac tgcgcttcat cgttggcgtc ctgaatagaa ccggggcgca gaccgtcgcc
    17701 cagcgacagc gaaacgtcat aagcggcaca gatttcacaa atttcgcgga agtgttgata
    17761 gaggaaattt tcctgatgat gggagaggca ccatttcgcc ataatcgaac cgccgcgcga
    17821 gacgataccg gtcaggcgtt tcgcggtcat cggcacatag cgcagcagca cccccgcatg
    17881 gatagtgaag taatccacac cttgctcggc ctgttccagc agcgtgtcgc ggaacgcttc
    17941 ccaggtaaga tcttcggcga tcccattaac cttctccagc gcctggtaga tcggcactgt
    18001 accaatcggc accgggctgt tacgcaaaat ccactcgcgg gtttcgtgaa tatagcgacc
    18061 ggtggagaga tccatcaccg tatccgctcc ccagcgcgtg gaccatacca gcttttccac
    18121 ttcttcttcg atggaagagg tgaccgccga gttgccgata ttggcgttaa cttttaccag
    18181 gaaattgcga ccaataatca tcggctccga ttccggatga ttaatgttgg ccgggataat
    18241 cgcacgtccg gcagcaactt catcacggac aaattccgca gtgatatttt ccggcagacg
    18301 tgcgccaaag ctcattcccg gatgctggtg gcgtaaaact tcgctacgga tgcgctcgcg
    18361 gcccatattc tcgcggatgg cgatgaattc catttccggt gtgatgatgc cctggcgggc
    18421 gtagtgcagt tgtgtgacgc ggcgtcctgc tttggcgcgt tttggcgtta atacgccgct
    18481 aaaacgcagc tcgtcgaggc catcatctgc cagccgcgct ttagtgtaat cggaactgcg
    18541 cacggtaagt tcttcggtat cgccgcgcgc atcgatccac ggctggcgta gttttgccag
    18601 cccttgctgc acgttaatgg cgatctgcgg atcgccatac gggccggagg tgtcgtagac
    18661 cggaatcgct tcgttttctt cgtactgcgg ctgttctttg ctaccgccaa ttagcgtcgg
    18721 gctaagctgg atctcacgca tcggcacgcg cacgccgggt tgtgtgccag tgatataaat
    18781 gcgttttgag ttgggaaagg cggtgccttc cagggtgtcg ataaaatgtt gggcctgggc
    18841 gcgttgttcg cgacgggtca gttttgttgc agacatagct cattccaaaa agttaaggac
    18901 gtggcttgtc agacgacgga tgaagcaaga gacgatcgcc gcaaaggcga tgcgatagca
    18961 gattaactct tgttcccttc gcaggtatta gcctgatcag gttccgcgga tcccgaataa
    19021 acggtctcag ccagttaagg cactccgaca agaaattagc cccacaaacg gggcgttgaa
    19081 tgtaaattac gcgttaacag cgcagaactc aagcaggatg tttgacgcgg gcggcgtcaa
    19141 gcaccagcag aatcagctta tcttccagca caaagcgcgc ttccagcgcc tcgccgatgt
    19201 cagataaaac ctgttgaaac tcaaggtaat tatcatgatc gatagcggtt tccaggctgg
    19261 aatcgtagta atccataatc tgttgggtat tggcttcgag ttgcggccaa atcttcgcgg
    19321 cgcgcgcgag ttgcccgttg ccttccagct tatgaagaat gcgctcataa atactgaaat
    19381 gcccggcaga caagtaatcg accaggctct gacaaaaatc atcaagggct ttttcgttta
    19441 gcctcatgta cgattctttg ccaggcttaa tgccaaccag attgtagtaa gccacgagca
    19501 gatgcttacg tacatgtagc cagcgatcaa ccagtttgtt acttcctctg acgcgctccg
    19561 tcaggttatc gagctggtta agcatgattg actccgcaag tttgtattca aaaactgctc
    19621 agtgagaaat gtaaaaacca tgttaaacat gccagtgatg caaaggtagt gcaagagcta
    19681 tggatcgtat aattgaaaaa ttagatcacg gctggtgggt cgtcagccat gaacaaaaat
    19741 tatggttgcc gaagggagaa ttgccatatg gcgaagcggc aaatttcgat cttgtgggtc
    19801 agcgcgcact acaaatcggc gaatggcagg gggaacctgt ttggttaata caacagcagc
    19861 ggcgtcacga tatggggtcg gtacgtcagg tcattgatct cgatgttggg ctgtttcaac
    19921 tggccggacg tggcgtgcaa ctggcggagt tttaccgatc gcataaatac tgtggttact
    19981 gcgggcatga aatgtatcca agcaaaaccg aatgggcgat gctgtgcagc cattgccgtg
    20041 agcgttacta cccgcaaatc gccccctgca ttattgttgc catccgtcgc gatgattcga
    20101 tcctcctcgc ccagcatacc cgccatcgta acggtgtcca tacggtactt gccggattcg
    20161 tcgaagtggg cgaaaccctc gagcaggcgg tcgcgcggga agtgatggaa gagagcggaa
    20221 ttaaagttaa aaacttgcgt tatgtgactt cccagccgtg gccgtttcct cagtctttaa
    20281 tgaccgcgtt tatggcggaa tatgacagcg gcgacatcgt gatcgacccg aaagaattgc
    20341 tcgaggcgaa ctggtatcgc tatgacgatt tgccgttact cccgccgccc ggcaccgtgg
    20401 cgcgccgtct gatagaagat acggtggcga tgtgtcgggc agagtacgag tgatgataca
    20461 ctgaccgcct gacgcactaa ggaacagcca aaatgaccga acttaaaaac gatcgttatc
    20521 tgcgggcgct gctgcgccag cccgttgatg tcactccagt atggatgatg cgccaggcgg
    20581 gtcgctatct accggaatat aaagccacgc gcgcccaggc gggcgatttt atgtcgctgt
    20641 gcaaaaacgc cgagctggcg tgtgaagtga ccttgcaacc gctgcgtcgc tacccgctgg
    20701 atgcggcgat cctcttttcc gatatcctca ccgtcccgga cgcgatgggg ttagggctct
    20761 attttgaagc cggagaaggt ccgcgtttta cctcgccagt cacctgcaaa gctgacgtcg
    20821 ataaactgcc aattccggac ccggaagatg aactggggta cgtgatgaac gcggtgcgta
    20881 ccattcgtcg cgaactgaaa ggcgaagtgc cgctgattgg tttttccggc agcccgtgga
    20941 cgctggcgac ctacatggtg gaaggcggca gcagcaaagc cttcaccgtg atcaaaaaaa
    21001 tgatgtatgc cgatccgcag gcgctgcacg ctctactcga taaactggcg aaaagcgtca
    21061 ctttgtatct gaatgcgcag attaaagccg gtgctcaggc tgtgatgatt ttcgacacct
    21121 ggggcggcgt gcttaccggg cgcgattatc aacagttctc gctttattac atgcataaaa
    21181 ttgttgatgg tttactgcgt gaaaacgacg gtcgccgcgt accggtcacg ctgtttacca
    21241 aaggcggcgg acagtggctg gaagccatgg cagaaaccgg ttgcgatgcg ctgggcctcg
    21301 actggacaac ggacatcgcc gatgcgcgcc gccgtgtggg caataaagtc gcgttgcagg
    21361 gtaatatgga tccgtcgatg ctgtacgcgc cgcctgcccg cattgaagaa gaagtagcga
    21421 ctatacttgc aggtttcggt cacggcgaag gtcatgtctt taaccttggt cacggcattc
    21481 atcaggatgt gccgccagaa catgctggcg tgttcgtgga ggcagtgcat cgattgtctg
    21541 aacagtatca ccgctaagga gcgattatgg atctcgcgtc attacgcgct caacaaatcg
    21601 aactggcttc ttctgtgatc cgcgaggatc gactcgataa ggatccgccg gatctgatcg
    21661 ccggagccga tgtcgggttt gagcagggcg gagaagtgac gcgagcggcg atggtgctgc
    21721 tgaaataccc ctcgcttgag ctggtggagt ataaagtggc ccgcatcgct accaccatgc
    21781 cttacattcc aggttttctt tccttccgcg aatatcctgc gctcctggca gcgtgggaga
    21841 tgctgtcaca aaagccggat ttagtgtttg tcgatggtca tgggatctcg catcctcgcc
    21901 gtcttggcgt cgccagccat tttggtttgc tggtggatgt gccgaccatt ggcgtagcga
    21961 aaaaacgact atgcggtaaa ttcgaaccgc tctccagcga accgggcgcg ctggccccac
    22021 tgatggataa aggcgagcag ctggcctggg tctggcgcag caaagcgcgc tgtaacccgt
    22081 tgtttatcgc taccggccat cgggtcagcg tggacagcgc gctggcgtgg gtacaacgct
    22141 gcatgaaagg ctatcgtctg ccggagccaa cgcgctgggc ggacgcggtg gcctcggaac
    22201 gtccggcgtt cgtgcgctat acagcaaatc agccctaatt caggtaaact gcggataatt
    22261 tccgtatttg agaactcaac atgttacaaa acccaattca tctgcgtctg gagcgcctgg
    22321 aaagctggca gcatgtcact ttcatggctt gcttatgcga acgcatgtac cccaattacg
    22381 ccatgttttg ccagcaaacc ggttttggcg atgggcaaat ttaccgccgt attctcgatc
    22441 tcatctggga aacgctgacc gtcaaagacg caaaagtaaa tttcgacagc caactggaga
    22501 aatttgaaga agcgattcct tcagccgacg atttcgatct gtacggcgtt tatccggcaa
    22561 tcgatgcatg cgtggcgtta agtgaactgg tccattcgcg tttgagtggt gaaacgctcg
    22621 aacacgcggt ggaagtgagt aagacctcca tcacgaccgt tgcgatgctg gaaatgactc
    22681 aggctggtcg cgaaatgagc gatgaagagc tcaaagaaaa cccagctgta gagcaagaat
    22741 gggacattca gtgggaaata ttccgacttt tagctgagtg cgaagaacgc gatatcgagc
    22801 tgataaaagg ccttagggca gacctgcgtg aggcgagtga gagcaatatt ggtataattt
    22861 ttcagcaata agaccagaaa acgtgattta acgcctgatt tgtcgtacct ggagtcttcc
    22921 ctttcgcccc ccgtctggtc tacatttggg gggcgaaaaa aagtggctat cggtgcgtgt
    22981 atgcaggaga gtgctattct ggcatttccg tcgcactcga tgcttagcaa gcgataaaca
    23041 cattgtaagg ataacttatg aacaagactc aactgattga tgtaattgca gagaaagcag
    23101 aactgtccaa aacccaggct aaagctgctc tggagtccac tctggctgca attactgagt
    23161 ctctgaaaga aggcgatgct gtacaactgg ttggtttcgg taccttcaaa gtgaaccacc
    23221 gcgctgagcg tactggccgc aacccgcaga ccggtaaaga aatcaaaatt gccgcagcta
    23281 acgtaccggc atttgtttct ggcaaggcac tgaaagacgc agttaagtaa gattgcgtgg
    23341 cagtgaacag ttttaacgaa ggggtggttt cacccctttt gtctttctgg cgtcgatcat
    23401 tgatgctggc tggcgctctg tttctcactg cctgtagcca caactcttca cttcctccct
    23461 ttaccgccag tggatttgct gaagaccagg gcgcggtacg catctggcga aaagacagcg
    23521 gcgataatgt gcatcttctt gccgtgttta gcccgtggcg cagtggcgat accacgacgc
    23581 gagagtatcg ctggcagggc gataacctca cgctcatcaa tatcaatgtt tacagcaaac
    23641 cgccggtgaa cattcgcgcg cgttttgacg atcgcggcga tctgagcttt atgcaacgtg
    23701 aatccgatgg ggaaaagcag cagctttcta acgaccaaat cgatttatac cgttatcgtg
    23761 ctgatcagat ccgccagatt agcgatgcct tacgtcaggg gagagtcgtg ctgcgccagg
    23821 ggcgctggca tgcgatggaa cagaccgtga ccacctgcga agggcaaacc attaaacctg
    23881 atttagattc gcaggcgata gcgcatatcg agcgccgcca gagccgctct tctgttgatg
    23941 tcagcgtggc atggctggaa gcgcccgaag gttcgcaatt actgttagtg gcaaactctg
    24001 atttctgtcg ctggcaaccc aacgagaaaa cgttctgatt taccagtggc ccatacccat
    24061 atgaccgcca ccaccgcagc cgccgtaacc cattccggta ccgcgcggaa tacccgcttc
    24121 agccatcgca atatctcgtt tcacccgtaa ctcatctaac gactgacgca aattctccat
    24181 ctctttggcg accgcgttaa ttttgctgct atccggtggg ttcgcggcta acagggcatt
    24241 gtattcataa cgcttcgtca ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc
    24301 attatggatt ttctgccacg ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg
    24361 ccacataccg tgtccgccgt gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag
    24421 cgccatcatt accagggcaa ttttcgtgtt ccgtttcatg gataatcctc cagtggctgt
    24481 cttacttcgg gtatagcatc ttccgtgcca acgatgaaac gctgatatga cgggtaatct
    24541 ggcatgataa acgagtaaaa atgactcgcc tgcggcgggt agcgagtcat ttttactcat
    24601 tgaaacttaa gcctttgtgt tacagcgcag ggtaagcgct gataaaagat ggcatgattt
    24661 ctgctgtcag aaagggatga gcaggcaaag aagaagatgc gttttatgca acgttctaaa
    24721 gactccttag ctaaatggtt aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg
    24781 ttgtttgcgg tgacggtgat tcgcgattat gggcgcgaga ctgccgctgc cagacaaacg
    24841 ctgctggaaa aaggcagtgt acttattcgt gctctcgaat ccggctcgcg cgtcggcatg
    24901 gggatgcgca tgcatcatgc gcagcagcag gccttactgg aagaaatggc cgggcagcct
    24961 ggagtacgtt ggtttgcggt cacggatgaa caaggaacaa tcgtgatgca tagcaactcc
    25021 ggcatggtgg gaaaacagct ttattccccg caggaaatgc agcagttaca tccgggagat
    25081 gaagaagtgt ggcggcggat cgatagcgca gacggcgagc ctgttctgga aatttatcgc
    25141 cagtttcaac cgatgtttgc tgctggaatg caccggatgc gccatatgca gcagtatgcc
    25201 gcgacaccac aagcaatttt catcgctttc gacgccagta atattgtgag tgccgaagat
    25261 cgtgagcaga gaaacaccct gattatcctc ttcgccctgg cgacggtctt gctggcaagc
    25321 gtattgtcat tcttctggta tcgccgctat ctgcgctcgc gccagcttct acaagatgaa
    25381 atgaagcgca aagagaagct ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa
    25441 atccgtaacc cactttcctc gattaaagga ctggcgaaat actttgccga gcgcgcgcct
    25501 gcagggggag aagcgcatca actggcgcag gtgatggcga aagaggccga ccgtttaaac
    25561 cgcgtggtaa gcgagttgct ggaactggtt aagccaacgc atctggcttt gcaggcggtg
    25621 gatctcaaca cgctgattaa ccactcatta cagctggtaa gccaggatgc aaacagccgg
    25681 gagatccagt tacgctttac cgccaacgac acattaccgg aaattcaggc cgatccggac
    25741 aggctgactc aggtcctgtt gaatctctat ctcaatgcta ttcaggcgat tggtcagcat
    25801 ggcgtgatta gcgtgacggc cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac
    25861 agcggtaagg gaattgcggc agatcagctt gaagccatct tcactccgta cttcaccacc
    25921 aaagccgaag gcaccggatt ggggctggcg gtcgtgcata atattgttga acaacacggt
    25981 ggtacaattc aggtcgcaag ccaggaggga aaaggctcaa cgttcaccct ctggcttccg
    26041 gtcaatatta cgcgtaagga cccacaagga tgacgcacga taatatcgat attctggtgg
    26101 tggatgatga cattagccac tgcactattt tgcaggcttt actgcgcggc tggggctata
    26161 acgtcgcgct ggcgaacagc gggcgacagg cgctggagca ggtgcgggaa caggtttttg
    26221 atcttgtgct ttgcgatgtg cgaatggcgg agatggacgg catcgccacg ctgaaagaga
    26281 tcaaagcgtt aaacccggca attccggtgc tgattatgac tgcgtactcc agcgtcgaga
    26341 cggcggtaga ggcactgaaa actggggcgc tggattatct catcaagccg ctggatttcg
    26401 ataacctaca ggcgacgctg gaaaaggcgc tcgcgcatac gcacagtatt gatgctgaaa
    26461 cacctgcggt gactgccagc cagttcggta tggtcggtaa aagcccggcg atgcaacacc
    26521 tgctcagtga aatcgccctc gtcgcgccat cggaagccac ggtgctgatc cacggcgatt
    26581 ccggcaccgg taaagagctg gtcgccaggg cgattcatgc cagtagcgca cgtagcgaaa
    26641 aaccgctggt aacgctcaac tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt
    26701 tcggtcacga aaaaggggcg ttcactggag ccgataaacg ccgggaaggg cgctttgttg
    26761 aggcggacgg cggcacgctg tttctcgatg aaattggcga tatctcgccg atgatgcagg
    26821 tgcgtctgct gcgtgcgatt caggagcgcg aagttcagcg tgtcggtagc aaccagacta
    26881 tctcggttga tgtccggttg attgcggcga cccatcgcga tcttgccgca gaggtgaatg
    26941 ccgggcgttt tcgccaggat ctctactatc gcctgaatgt ggtggcgatt gaagtaccat
    27001 cgctgcggca acggcgggaa gatatccctc tgctggctgg ccattttctt cagcgctttg
    27061 ccgagcgtaa tcgcaaggcg gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc
    27121 actacgactg gccgggaaat attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc
    27181 tgctgaccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc ggtacgccga
    27241 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtcgaaaaa gaagtgattc
    27301 tggcggcact ggagaaaacg ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca
    27361 cgcgcaaaac gctattggca aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc
    27421 agccgatatc tttccggcag aagcagtcgt cccagtgaat atcggtcatt aaggcataag
    27481 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27541 cgctggtcac tacctgctcg ttatccgcca gtttcgtgcc cgcgtggaac actttgccgt
    27601 cagccacttc ttccagcggc aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat
    27661 atccacccgc agccatcacc acgccgagag aagcacgttc atcccactct gacgttttct
    27721 cgtccagttt gccttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27781 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27841 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    27901 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacttcat
    27961 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt
    28021 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28081 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28141 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28201 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28261 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28321 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28381 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcagcgcgg aaggtatcga
    28441 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28501 gtgcgaaatc caacagcgcc gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg
    28561 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28621 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28681 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28741 ctcgtcggcg gcggcaatca cttcgtcatc acggatagaa ccgccaggct ggattacaca
    28801 ggtcacgccc gcagccgcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28861 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    28921 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    28981 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcacg
    29041 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29101 gcccagatcg cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat cgaggcccgg
    29161 aacacgctcg ccccactgac cgcaggtcag aacgcgtaca ttctgtttgg cggcggtgat
    29221 tttcagggct tcttcgctgg cggacggcgc aataatcact tcaacaaact gacgagaaat
    29281 gatggcctgt gcggtttccg catccagctc gcggttaaag gcaatgatgc cgccgaatgc
    29341 ggaggtcggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29401 cacgccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29461 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29521 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29581 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29641 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg taagccggaa ccatgctgcc
    29701 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29761 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29821 gtcgtagtcg ctgctcttca ccacgattga gacatcttta tggttcttgg cggcggagcg
    29881 caccatcgtt gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcaaccttc
    29941 acgggccacg gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30001 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30061 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30121 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30181 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30241 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30301 gactattaca gagagcgtta gctgaatttt tcgcaaaaaa ctcagctaac gcccctaacg
    30361 gggcatcttt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30421 ttctctttca atggtgatca caattttgac agtggttacc gtgggaaaaa tacagaaatt
    30481 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30541 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30601 taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30661 gaaga
//
LOCUS       DAPGRN010000056        30236 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPGRN010000056 DAPGRN010000000
VERSION     DAPGRN010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.056"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..1686
                     /locus_tag="RHW33_002600"
     CDS             103..1686
                     /locus_tag="RHW33_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV1646088.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRG
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(1960..3813)
                     /gene="asmA"
                     /locus_tag="RHW33_002601"
     CDS             complement(1960..3813)
                     /gene="asmA"
                     /locus_tag="RHW33_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV1646089.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLGGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTSQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3835..4416)
                     /gene="dcd"
                     /locus_tag="RHW33_002602"
     CDS             complement(3835..4416)
                     /gene="dcd"
                     /locus_tag="RHW33_002602"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV1646090.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4508..5149)
                     /gene="udk"
                     /locus_tag="RHW33_002603"
     CDS             complement(4508..5149)
                     /gene="udk"
                     /locus_tag="RHW33_002603"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV1646091.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5467..8784
                     /locus_tag="RHW33_002604"
     CDS             5467..8784
                     /locus_tag="RHW33_002604"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1646092.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNELLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLGGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8893..9741)
                     /gene="alkA"
                     /locus_tag="RHW33_002605"
     CDS             complement(8893..9741)
                     /gene="alkA"
                     /locus_tag="RHW33_002605"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV1646093.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVHGALGRLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRLYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9875..11227
                     /gene="yegD"
                     /locus_tag="RHW33_002606"
     CDS             9875..11227
                     /gene="yegD"
                     /locus_tag="RHW33_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1646094.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPETITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11240..13180)
                     /gene="yegI"
                     /locus_tag="RHW33_002607"
     CDS             complement(11240..13180)
                     /gene="yegI"
                     /locus_tag="RHW33_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV1646095.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATARPTAKAWVAALDSLRQQLKKCTVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FIDLGEEVITTGGNFVLAKVWAMVMASVAPPAVQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAESRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDAASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPAERQAVMAKMAAKRHRLESALTVDATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13177..13938)
                     /locus_tag="RHW33_002608"
     CDS             complement(13177..13938)
                     /locus_tag="RHW33_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDV1646096.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13935..14594)
                     /locus_tag="RHW33_002609"
     CDS             complement(13935..14594)
                     /locus_tag="RHW33_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV1646097.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14815..14874)
                     /gene="ibsA"
                     /locus_tag="RHW33_002610"
     CDS             complement(14815..14874)
                     /gene="ibsA"
                     /locus_tag="RHW33_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV1646098.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15147..15203)
                     /locus_tag="RHW33_002611"
     CDS             complement(15147..15203)
                     /locus_tag="RHW33_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1646099.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            complement(15475..15531)
                     /locus_tag="RHW33_002612"
     CDS             complement(15475..15531)
                     /locus_tag="RHW33_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV1646100.1"
                     /translation="MMKILIIVVLLVISYPAY"
     gene            15810..17057
                     /gene="mdtA"
                     /locus_tag="RHW33_002613"
     CDS             15810..17057
                     /gene="mdtA"
                     /locus_tag="RHW33_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV1646101.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLTNARHDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKSGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17057..20179
                     /gene="mdtB"
                     /locus_tag="RHW33_002614"
     CDS             17057..20179
                     /gene="mdtB"
                     /locus_tag="RHW33_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV1646102.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMNSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWAFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20180..23257
                     /gene="mdtC"
                     /locus_tag="RHW33_002615"
     CDS             20180..23257
                     /gene="mdtC"
                     /locus_tag="RHW33_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV1646103.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNDGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23258..24673
                     /locus_tag="RHW33_002616"
     CDS             23258..24673
                     /locus_tag="RHW33_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1646104.1"
                     /translation="MTDLSDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLTGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24670..26073
                     /gene="baeS"
                     /locus_tag="RHW33_002617"
     CDS             24670..26073
                     /gene="baeS"
                     /locus_tag="RHW33_002617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV1646105.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26070..26792
                     /gene="baeR"
                     /locus_tag="RHW33_002618"
     CDS             26070..26792
                     /gene="baeR"
                     /locus_tag="RHW33_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV1646106.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLSYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKLLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26983..27315
                     /locus_tag="RHW33_002619"
     CDS             26983..27315
                     /locus_tag="RHW33_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV1646107.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTARNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27558..27809
                     /locus_tag="RHW33_002620"
     CDS             27558..27809
                     /locus_tag="RHW33_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003915633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV1646108.1"
                     /translation="MARTMTVDLGDELREFIESLIESGDYRTQSEVIRESLRLLREKQ
                     AESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27811..28107
                     /locus_tag="RHW33_002621"
     CDS             27811..28107
                     /locus_tag="RHW33_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1646109.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28210..29571
                     /gene="yegQ"
                     /locus_tag="RHW33_002622"
     CDS             28210..29571
                     /gene="yegQ"
                     /locus_tag="RHW33_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV1646110.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDEVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29901..30218)
                     /locus_tag="RHW33_002623"
     CDS             complement(29901..30218)
                     /locus_tag="RHW33_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646111.1"
                     /translation="MNKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLSRGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6959 a         8073 c         7984 g         7220 t
ORIGIN      
        1 ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga
       61 attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat
      121 ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat
      181 aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cggccgcgca
      241 cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc
      301 tggctggtca ccctgacgca accgctgttc agcttccgct cgtttacctt tagcgcccgc
      361 gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac
      421 gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg
      481 ggggtagtga cacaaattgt ggtactggac gccatcttct cactcgactc agtgattacc
      541 gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc
      601 ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt
      661 attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc
      721 ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggcg
      781 ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca gacgctacgc
      841 cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg
      901 gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt taatccgcag
      961 gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtactgtcag cagcattatg
     1021 acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagacga gatccgccag
     1081 ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat
     1141 ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt cactgcgtgg cgaaccgctc
     1201 aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacct
     1261 gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga tgagtttggc
     1321 tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg
     1381 aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga cggttcctgg
     1441 acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct gccgctggat
     1501 gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca gcgtattcca
     1561 aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc
     1621 catcgcgtgc agaaggtgca gattattccg ctgcataagg atggcgagtt ggagtacgag
     1681 gtgtgatggt atcggtcatt ttacccgata gccgaaagga ataagatgag ggaaccgcgc
     1741 cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg
     1801 gcacgttcac cggcttgagc gttccatagt agtgaggaac atcaagtgaa cggaacagcc
     1861 ttcgatctta catattcaga atataatggt gccgccgcga acgcctgatg cgacgcttta
     1921 cgcgtcttat caggcctaca ggacaggtca cctggtaacc tacatcttct ccagcaactt
     1981 cttcacatct ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg
     2041 tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcagac tgtaattgag
     2101 ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag
     2161 tttgctttcc ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg
     2221 atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt gaccttgcat
     2281 gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg
     2341 cgttacgtta tcgaagtttt cagcggcctt cacatcacca ccattacgtt ctaccgcttg
     2401 ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc
     2461 ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc
     2521 agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc
     2581 aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc
     2641 cagcgtgccc ggcagtgaaa cctgtccacc gttaagtttg ccctgcagtt gagtaatttc
     2701 cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca
     2761 gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc cctgataggc
     2821 cggttcatca atacgcgaag aaatgaccgg gcgcggcaac gttgattggc tctgcccctg
     2881 ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt tgtcaagatt
     2941 cagttgcggg aattgcagtc tcagctgcca ttccggtttc tctgtcagcg tgacctgtgc
     3001 ttgcccgctc agcgtactgt cattggcggt caagctgatt tggttaaatg aaaggcgttt
     3061 atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt gttttggtaa
     3121 atcggcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat catgcggata
     3181 atcagaagca tctaccgtac cgttaaggga tattgtgaga tcgcgctgat cgcgattaac
     3241 ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt gcaggcggat
     3301 attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc tgtccgccac
     3361 cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt
     3421 gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg acgtcagttg
     3481 gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa
     3541 aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc
     3601 ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg
     3661 cccgccgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg
     3721 gaaatcattc ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccag
     3781 gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc
     3841 tttatcgatt cggctggcta ccgcgccctg ctggttgcga tacttcgcat cttcacggcg
     3901 gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat
     3961 gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcagcc
     4021 agaccagccc ggatcgatac ggtgtgcggt gacgtgcacc atcagcccca gacgcgccaa
     4081 agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagcgt
     4141 caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat
     4201 ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc
     4261 gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaggc gtacatccac
     4321 cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc
     4381 aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcgcatca
     4441 ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg tacacaattt
     4501 atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt
     4561 ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca
     4621 ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac
     4681 gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga
     4741 aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga
     4801 gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgtg tgttctacgt
     4861 aactgtaaac cggcaggtca attgccgaac cgcgtttcaa cgcttgtaaa tgctcaagca
     4921 acaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg
     4981 acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac
     5041 cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg
     5101 atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata tatttagcga
     5161 cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg
     5221 atttcatccg cctgatacca gtcgaatagc gttgccgcgc gctcagagtt aattgttgac
     5281 gaagaattcc cggtggcaaa ttacgttgat cagttttata caaggtaaaa aatgttatac
     5341 gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta
     5401 aaacttttgt actagcataa acacagaaac gaatactggc gacctggcct tgcggataaa
     5461 gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac
     5521 ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga gctttcgcag
     5581 tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat
     5641 cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg aaatatcgcc
     5701 gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac catcaatatt
     5761 gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta taaccccttg
     5821 caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc acctctgttg
     5881 gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata
     5941 tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa
     6001 ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta
     6061 gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc
     6121 attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ttttttgatc
     6181 ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct
     6241 acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg
     6301 cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc
     6361 gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat ggcgttagtg
     6421 ggcaccgagg gacaatggct gcaatccaac aaagcactct gccagtttct cggttacagt
     6481 caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa
     6541 gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa
     6601 cgttactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc tctggtgcgc
     6661 cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa
     6721 cgcaccgaac aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc
     6781 gggattggca tctgggagtg ggagctgaag ccgaatattt ttagctggga taagcggatg
     6841 ttcgaactgt atgaaattcc tccgcatatc aaaccgaact ggcaggtgtg gtacgagtgc
     6901 gtgctgccgg aagatcgtca acacgccgaa aaagtgattc gtgattcgtt gcaatcacgc
     6961 tcgcccttta aactggaatt tcgcattgcc gtgaaagatg gcatccgcca tatccgcgcc
     7021 ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg
     7081 gatatgaccg aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac
     7141 attacgctgg actccattgg cgaagccgta gtctgtattg atatggcgat gaaaattacc
     7201 tttatgaatc cggtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt
     7261 ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat ggagaacatt
     7321 tacagtgctg acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggagc
     7381 ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat
     7441 attggttcgg ttctggtgat tcaggacgtc accgaatcac gcaaaatgct acgccagctg
     7501 agctacagcg cctcccatga tgcactaacg catctcgcca atcgcgccag ttttgagaag
     7561 caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc actggtgttt
     7621 atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggcgacgct
     7681 ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga cgtgctggcg
     7741 cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga aagcgctcgt
     7801 tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg ggaaggccgg
     7861 gtacatcggg taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg
     7921 gctgaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggccggggc
     7981 cgggtgacga tttacgaacc gcagcaagct gccgcacata gcgaacgggc ggcgatgtcg
     8041 cttgatgaac agtggcggat gattaaagag aatcagttga tgatgctcgc ccacggtgtc
     8101 gcttcgccac ggatcccgga agcgcgtaat ttgtggctga tttcacttaa gctctggagt
     8161 tgcgaaggcg agattattga tgaacaaaca tttcgtcgta gcttcagcga tccggcgctt
     8221 agccatgctc ttgaccgccg ggtattccac gaatttttcc agcaggccgc aaaagcggtt
     8281 gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccaca
     8341 ctggtgaatg aactgcttga gcagctggaa aacagccctc taccaccacg gttattacat
     8401 ctgattattc cggcagaagc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg
     8461 gggggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg
     8521 aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt
     8581 aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg
     8641 aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc
     8701 gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat
     8761 agtagttatt tcgcgattaa ctgaggcgtg cttccccatc gcctgatgcg acgctaacgc
     8821 gtcttatcat gcctacaaat cgctcattcc ccaggccgga taaggcgctc gcaccgcatc
     8881 cggcgaccaa cgtcatgctt cgtctggttg ccagccttcc gtataccaga tatgcaacag
     8941 cgcataagaa cgccagggct tccagcgttc ggcataaagg cggatttgtg ccggtgtcat
     9001 tcccggaaat cgctgtttaa tcagataatc atccggcagg aaaacatctt tcgcctgcca
     9061 gccacgtaaa gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt
     9121 tttcatcgct tgttctacgt cgcccggtat tgtcattggt aaggtgccct ccagcgccgc
     9181 atttgccaga tgaatcagcg cctctgcccg tttcaacggc atgcctaacg cttttaatgc
     9241 ctgcgggtcg gctgctgcca gccgctgagg cgtggggaag cagatatact ccggaaaatc
     9301 atccagccgt tcgccataaa gctgtaccac tttggcagtc aattttgccg ccatcgccac
     9361 gctcaccagt tggcctaaaa tcgcccgcac gccctgctca aaagcatcaa cacagccggg
     9421 taaacgcaat ccgggccgcg ccgcgcctaa cctgcccaac gcaccgtgaa caatttgtgg
     9481 gttacattgc agatcaaaca ggcggctcat tttcgccaga cactctgcgg caacaggttc
     9541 taaacctgca cttaaattta tgtgcagagt atggcgggct atatccggaa tagcagtcac
     9601 tacgccgcga tattcgccca ccgccagact acgggcataa taattgtccg cgaccgtttc
     9661 cacaccactc actgcacggg cggcgagaaa tcccaacatc cacgaccagt catacggcgg
     9721 ctgccagttc agggtataca tcgcatctcc ttattcatcc gctttcagca taaacgttat
     9781 tcagacgcta cgctttgctt tcatattccg gttgtcgcga cggcaacatt tcgctaaagt
     9841 cacgcccctt cttcaccggc atggggatta attcgtgttt attggttttg attacggtac
     9901 agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg caattactga aaatggaaaa
     9961 cgacagcacg ctgctgcctt caatgctttg cgcgccaacg cgtgaggcgg taagcgaatg
    10021 gctgtaccgc catcatgatg ttccggcaga cgacgatgaa acgcaggcgc tgttacgtcg
    10081 ggcgattcgt tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg
    10141 tctttcctca ctggcacagt acattgatga tccagaagaa gtgtggtttg tgaaatcacc
    10201 aaaatcgttc ctcggtgcca gcggcttaaa accacagcag gtagcgctgt ttgaggatct
    10261 ggtctgcgca atgatgttgc acattcgcca gcaggcgcag gcacagctgc cagaaacgat
    10321 tactcaggcg gtgatcggtc gcccgatcaa cttccagggg cttggcggcg atgaagcaaa
    10381 cacccaggcg caagggattc tggaacgcgc ggcgaagcgt gccgggttta aggatgtggt
    10441 attccagtac gagccggtag cggcagggct ggattacgaa gcgactttgc aggaagaaaa
    10501 acgggtgctg gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg
    10561 gccgcagtgg cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca gtggttgccg
    10621 tattggcggt aacgatctgg atatcgcgct ggcgtttaaa aacctgatgc cgttactggg
    10681 catgggtggc gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt ggaatgcggt
    10741 tgccatcaac gacgttcctg cgcagagtga tttctacagt agtgccaacg gtcgtctgct
    10801 taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg gccctgttac agaaagtctg
    10861 gcgtcagcgt ttaagttatc gcctggtgcg tagcgcagaa gagagcaaaa ttgcgctttc
    10921 aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc gatgaactgg ctacgctgat
    10981 tagccagcaa gggctggaaa gcgccctcag ccagccactg acgcggattc tggaacaggt
    11041 gcaactggcc ctggataacg cccaggaaaa accggacgtt atctacctga ccggcggaag
    11101 cgcccgttct ccgctgatta aaaaagcgct ggcagaacag ttgccgggca ttccgattgc
    11161 aggcggcgat gactttggct ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt
    11221 tcgttaattg aaaaatggct cagcagcggc ttaaatctgc ctgcgcctga gccagttttt
    11281 ctgccgcctg acgtaacggt tccatcaacg gcatggtccg tgctggagca tgaaggcgga
    11341 atcgctgcaa ctctgtcgcg tcgacagtca acgccgattc cagccgatgt cgtttggcgg
    11401 ccattttcgc cattaccgct tgtctttctg caggcgttac cgcttcgttc ggcctgaaca
    11461 caaaacggcg ttcacagctc gctttccagt cgatgaccgc ctgggtcaga tgatcaccaa
    11521 accctttaac ttgcttaacc ccacgacggg taacatccgc ggctgtttca ataccaaaag
    11581 accgtaacgc cgctttacgc gcagggccaa cgccgggaat agaggcagca tcaataaaaa
    11641 atccctccag aaacttctgc ttctgccgtt cccttgcggt gtcgtgaagt gctgccagag
    11701 cacgtttttc ttcttccggt aacccgagca tttcgtcctt cattttttcg agcatcgtcc
    11761 gtttggcgat aaaaccttcc agcccgccca actgttggat ctggctgact aaatggtcat
    11821 agtccatttt cgcgcgatta aatgcctcac ggcgttgctg gatttctgct ttataagctt
    11881 tgcttgtcag actgccaata atccagatag ccgccagcac aggaaccaaa ataatataac
    11941 gcgattctgc ctgaaggccg caaagcaaca gcgataacgc tgacagtgcg atctcaagca
    12001 gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg cctgccagtc ggttggaaat
    12061 gatcgggtaa tggcaattgc actgctggcg gtgctactga cgccatcacc atcgcccaga
    12121 ctttcgccag cacaaaattc ccgccggtgg taatgacctc ttcgccgaga tcaataaaat
    12181 agataacgcc ttgattatcc agcgtacacc acgggcagtc agtcaaatga gcgggataaa
    12241 catgcattgc cgaaacggta catttcttta actgttggcg tagagagtcc agtgccgcta
    12301 cccacgcttt tgccgtcgga cgcgcggttg ccacgccact ttccgtgaat gcctgctgaa
    12361 acatggcttc aacatcgccc ggtaacatcg acagcggaat cgatcgtggc ggcggtttta
    12421 aaccacgtcg ctggttgtct gacgcgtagg cataacggaa atgggcaata tccgtctcca
    12481 gcgcattacc cgcatcagag ataagcggca caccagaata aggatgccgc ccaccaaaca
    12541 agacgtgaaa aatcagcaac gcaaggccaa aattatcgtg attcgcggta cgttcaaaac
    12601 cgacaaatga tggcaaggtt tgtagctctg gcggcgtaaa atgcgacacg ccgacttcgc
    12661 ataaatgcag tgtgccattg gcgttaatct gaaaggagtc actatcgatc aacaccactt
    12721 tgctgtcgcg acctaccata aagctgttct ggtttacgtc ccccacgacg tgcccgtgct
    12781 cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac atagagtaga aaatcccacg
    12841 cacaatgagg gtaactctgg cgacgatgtg ccgggctata gatcatatga atcggttctt
    12901 taccagaaac ttttggcatc ataaaaccga taacttttcc gcctcgccca ccgtgaagcg
    12961 ttgcctgcgg ccaggcgaca taattcaaca actgcgcgtc agctgtcgca gccataaagg
    13021 caagtttgtc ctgttttaag gcgggtggcg gcgtgtgata aatcttggcg acgctatcgg
    13081 caaactcctc gatatcataa accgcacctt caccgccttt gcccagttca cggcctggcg
    13141 taacgcactc accagtagca gtatataaag tgggtttcat ggtaaccaca gcgccagtgc
    13201 caacgtttta tcatcatcgg tacgctcgtt taccgccggg ctcgacaaaa actgctttaa
    13261 caattcaggt aataaatcga gttgttcctg cgttgctgcc gccagtccat tgaaaaacgg
    13321 ggtaaagaaa ggcacatgcg gagaattatc cagcatattt aacgccagtc gctgaatacc
    13381 atccgtaaat gctgcaactt tatgcgcacg ctcagtgctg gtgaaagttt ccagtctgga
    13441 aacagcatct tcatcgctaa tgaaatgcgt catattggca tattcaccca ccatcggcgt
    13501 gagcggcagt tccagaccac gaccaaaatc caccactaca ccgccgtcgc caatttgcat
    13561 aatcaaggtg ccgttagccg atgaaatcaa tcccagaaac gtgcaggcaa aatcgcgaac
    13621 tgctaatgct ttggcttctg cctcggcaaa aagtcgttga cgaatggtca gtaccatatt
    13681 tgtggcgagg acatcattaa gccccaattc accgtcctgt actttttgca ccataaacgc
    13741 catcgcttca ttgacggcga gcttcgcgcc ttctcctccc tgcgaaacgc tacctgcgcc
    13801 atcagcaagg aacatcacca gtaatggctg ctggtcattt agccaggcaa cctgcatctg
    13861 gcaggcatcc tgacaaggta aatcagcgct aatgtgcgac gttccaacag cggatgcata
    13921 aaccagacgc cagctcacac tgacgtccag ccttttggcg cttccagcac aacttccgtt
    13981 cccggcgtgg agcgggaaac cgaacgcagc gaactggata accagctaaa cagttcacgg
    14041 aattgtaatc cctgcagtgg caaaggctga cgaacgctga tttgtgccag agtcttcata
    14101 tccgccccct gaacaccgat ggaaaagaag gcaaacttct tatcttcttc cccctgaaac
    14161 actttgttgg cggcagcctg ccactcatcg gttggagcgc catcggtgat caggaaaatc
    14221 cacgggcgat aatacgaaat accatttgcg cggtactcac gcttcctctc ttcaaccata
    14281 tccagggctt tggtaatggc ggcccccata ggcgtatccc cctgggcaaa caggatgggc
    14341 gggaaaaagt tagcggcgct ggtaaagggt tgttccacat gcaccgggcc gaacgtcaca
    14401 atgccgagtt ccactctttt taaagccagc ggatcagcaa gcagttcatc acgaaaggta
    14461 accaatccgg cgttaagttc attaataggt ctgccgctca tcgagccaga aacatccagc
    14521 agcagaatgc aggggcaacg tggttccggg ttactggcaa aatcgctggt ggcaaacgtg
    14581 atttgttcgc tcataagaaa tcctttctta gagaattatt gaggtccggt atcataccga
    14641 ctgcaataat gaaataagag caaacgaaga aactttcaga attagtcccg gcggcgcaga
    14701 aaattttgct attcgcctcg caagaacgga gagccgtcga ttgacactaa gggcggagtg
    14761 acataatttc aggagtgagg gttagggaga ggtttccccc tccccctggt gttcttagta
    14821 agcctggaag ctaatcacta agagtatcac cagtatgatg acgtgcttca tcataaccct
    14881 ttccttattt aaggcccctt cctcgggagg ggctttcccg tttcagcatc ccgctgaaat
    14941 cgtcggctta cctcctttcg ccctgaatac agtctatcgc tgacttgcgg ataaaatagt
    15001 ttcctctgtg attattgcgt aacgtctcgc aaagaatatt tgctggcagg atcgcagact
    15061 gcaaagcctg cagattgaca atcttatcgt gaaggcatac tttcaggagt gagggtagag
    15121 cggggttttc ccccgccctg gtagtcttag taagccggga agcttatgac taagagcacc
    15181 acgatgatga tccacttcat catgaccctt tccttattta agaccccttc ctcgggaggg
    15241 gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc cctgaattca
    15301 gtctatcgct gacttgcgga taaaatagtt tcctctgtga ttattgcgta acgtctcgca
    15361 aagaatattt gctggcagga tcgcagactg caaagcctgc ggattgacat tctgaacgtg
    15421 aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt agtcttagta
    15481 tgctgggtag ctaatcacta agagcaccac gatgatgagt atcttcatca tgaccctttc
    15541 cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt
    15601 cggcttacct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata aaatagtttc
    15661 ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgcc attcagacga ttcccgatag
    15721 tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta tcattccgcg
    15781 aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc cgttgggtaa
    15841 tcgtaatcgt ggtggttatc gccgccatcg ccgcattctg gttctggcaa ggccgcaatg
    15901 actcccagag tgcagcccca ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc
    15961 gccgtggtat gcgttccggc ccattagccc cggttcaggc ggcgaccgcc gtagaacagg
    16021 cagtaccgcg ttacctcacc gggcttggca ctattaccgc tgctaatact gttacggtgc
    16081 gtagccgcgt ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag
    16141 caggcgattt actggcagaa attgacccca gccagttcaa agttgcatta gcacaagccc
    16201 agggccaact ggcaaaagat aaagccacgc ttaccaacgc ccgccatgat ctggcgcgtt
    16261 atcagcaact ggcaaaaacc aatctcgtct cccgccagga gctggatgcc caacaagcgc
    16321 tggtcagtga aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagc
    16381 tgcaactcga ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcagg
    16441 ttgatgttgg taaccaaatc tccagtggtg ataccaccgg gatcgtggtg atcacccaga
    16501 cgcatcctat cgatttgctc tttaccctgc cggaaagcga tatcgctacc gtcgtgcagg
    16561 cgcagaaatc cggaaaaccg ctggtggtag aagcctggga tcgcaccaac tcgaagaaat
    16621 taagtgaagg cacgctgtta agcctcgata accaaatcga tgccacaacc ggtacgatta
    16681 aagtgaaagc acgctttaat aatcaggatg atgcgctgtt tcccaatcag tttgttaacg
    16741 cgcgcatgtt agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa
    16801 tgggcaatga aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc
    16861 tggtgacgcc gggcattcag gacagtcaga aagtggtgat ccgcgcaggt atttctgcgg
    16921 gcgatcgcgt ggtgacagac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg
    16981 tggaagccca gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa
    17041 aaggagctcg ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct
    17101 gtttattatg cgtcctgtgg ccaccacgct gctgatggtc gcgatcttac tcgccgggat
    17161 tatcggttat cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca
    17221 ggtggtcacg ctctacccag gtgcgagccc ggatgtcatg aactctgccg ttaccgcgcc
    17281 gctcgaacgc cagttcgggc agatgtctgg tctgaaacag atgtcgtcgc aaagttccgg
    17341 cggtgcgtca gttatcactt tgcagttcca gctaacatta ccgctggatg tcgccgagca
    17401 ggaagtacag gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc
    17461 gccggtttac agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgttacctc
    17521 aaccgccatg ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat
    17581 ctcgcagatt tccggcgtcg gcctggtgac gctatccggc ggccagcgtc cggctgttcg
    17641 cgtcaaactt aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtacgcac
    17701 cgccattacc ggcgctaacg ttaactcggc taaaggtagc cttgacggcc cttcccgtgc
    17761 ggttacgctt tccgctaacg accagatgca atccgccgaa gagtatcgcc agctaatcat
    17821 cgcctaccag aacggcgcgc cgattcgtct gggcgatgtc gcaaccgtag agcaaggtgc
    17881 agaaaacagc tggctcggcg catgggcgaa caaagaacag gccattgtga tgaatgttca
    17941 gcgccagccc ggtgctaaca ttatctccac cgccgacagc atccggcaga tgctgccaca
    18001 gctcaccgag agtttgccga aatcggtgaa ggtgacggta ctttctgacc gtaccaccaa
    18061 tatccgcgca tctgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt
    18121 catgattatc tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgccgt
    18181 gccgctgtcg ttaatcggca cttttgcggt tatggtgttt ctcgattttt caatcaataa
    18241 cctgacgctg atggcgttaa ctatcgccac cgggttcgtg gtcgatgacg ccatcgtggt
    18301 gatcgaaaac atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa
    18361 aggcgcaggt gaaatcggct ttaccattat ctcgctgacc ttctcgctga ttgcggtgtt
    18421 gatcccactg ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac
    18481 cctggcggta gcgattttaa tctcagccgt ggtgtcgcta accctgacac cgatgatgtg
    18541 cgcacggatg ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga
    18601 aaaaatgttc gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca
    18661 tccgtggctg accttaagcg tggcgctcag cacgctgctg cttagcgttc tattgtgggc
    18721 gttcattccg aaaggtttct tcccggtaca ggataacggc attattcagg gcactttgca
    18781 ggcaccgcaa tccagctcct ttgccaatat ggcccagcgg caacgccagg tcgcggacgt
    18841 gattttgcag gatccggcag tgcaaagtct gacctcattt gttggcgttg atggcactaa
    18901 cccatcgctg aacagtgcgc gtttgcagat caacctcaaa ccgctggatg aacgtgatga
    18961 tcgggtgcaa aaagtcatcg cccgtctgca aacggcggtg gataaagtgc cgggcgtcga
    19021 tctcttcctg caaccaacgc aggacctgac catcgatact caggtcagcc gcacccagta
    19081 ccagtttacc ttgcaggcca cgtcactgga tgcgctcagt acctgggtgc ctcagttgat
    19141 ggaaaaactc cagcagctgc cacagctttc tgatgtctcc agcgactggc aggacaaagg
    19201 gctggtggcg tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc
    19261 ggacgtcgat aacgccctgt acaacgcatt tggtcagcgg ctgatttcca ctatttatac
    19321 tcaggctaac cagtatcgcg tggtgctgga gcacaacacc gaaaataccc cagggcttgc
    19381 ggcgctggat actattcgcc tgaccagcag cgacggtggc gtggttccgc taagttcgat
    19441 tgccaaaatt gagcagcgtt ttgcaccgct ctccatcaac catctggatc agttcccggt
    19501 aacgaccatc tcctttaacg tgccagataa ctattcgctg ggcgatgcgg tgcaggcgat
    19561 tatggacacc gaaaagacgc tgaatctgcc ggtggatatc accacgcagt tccagggcag
    19621 caccctcgcc ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt
    19681 ggcgatgtat atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct
    19741 ctcgacgcta cccacggcag gtgttggcgc cctgctggcg ttaatgattg ccggtagcga
    19801 actggatgtg attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc
    19861 catcatgatg atcgacttcg cgctggctgc tgaacgcgag caaggcatgt cgccgcgcga
    19921 ggcaatctac caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ctctggcggc
    19981 actgcttggc gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc
    20041 gttaggtatc ggcatggtcg gcggcctgat tgtcagccag gtgctgacgc tgtttaccac
    20101 gccggtgatt tatttgctgt tcgaccgcct ggcattgtgg accaaaagcc gctttgcccg
    20161 tcatgaagag gaggcgtaag tgaagttttt tgccctcttc atttaccgcc cggtggcgac
    20221 gattttactg tcggttgcca ttaccctgtg cggcatactg ggtttccgta tgctgccggt
    20281 cgccccgctg ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc
    20341 gtcgccggag acaatggcgt cttccgttgc cacgccgctg gagcgctcac ttgggcgcat
    20401 tgccggggtc agtgaaatga cctccagcag ttcgctcggc agcacgcgta ttattttgca
    20461 gtttgatttt gaccgggata tcaacggtgc ggcgcgtgat gtgcaggcgg cgatcaacgc
    20521 cgcacaaagt ctgctgccca gtgggatgcc cagccgcccg acttatcgca aagcgaaccc
    20581 gtcggatgcg ccaattatga tcctgacgct aacctccgat acttattcgc agggtgaatt
    20641 gtacgatttc gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgtcggtga
    20701 tgtcgatgtc ggcggcagct cgctgcccgc cgtgcgcgtc gggctgaacc cgcaggcgct
    20761 gtttaatcag ggcgtgtcgc tggatgacgt acgcactgcc atcagcaatg ccaacgtgcg
    20821 taaaccgcag ggggcgctgg aagatggcac tcaccgctgg cagatccaga ccaatgatga
    20881 gctaaaaacc gccgctgaat atcagccgtt gattattcac tacaacaacg gcggcgcggt
    20941 tcgtctgggc gatgtggcga cggtgaccga ctcagtgcag gatgtgcgca acgccgggat
    21001 gaccaacgcc aaaccggcta ttttgctgat gatccgcaag ttgccggaag ccaatattat
    21061 ccagacggtt gacagcatcc gggcaaaatt accggagttg caggaaacca ttccggcggc
    21121 gattgatctg caaattgccc aggatcgctc ccccaccatt cgcgcctcgc tggaagaagt
    21181 cgagcaaacg ctgattatct cggtggcgct ggtgattctg gtggtgtttt tattcctgcg
    21241 ctcgggtcgc gccactatta ttcccgccgt tgcggtgccg gtttcgctga ttggtacgtt
    21301 tgcggcgatg tacctgtgcg gattcagtct caataacctt tcgttaatgg cgctcaccat
    21361 cgctactggt ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg cacgtcatct
    21421 ggaagcggga atgaaaccgt tgcaagccgc actgcaaggt actcgcgaag tcggttttac
    21481 ggtgctgtcg atgagtctgt cactggtggc ggtgttcctg ccgctgttgt tgatgggcgg
    21541 attgccgggg cgtctgttac gcgaatttgc cgtgacgctt tctgtcgcca ttggtatttc
    21601 gttactggtt tccctgacgc taacgccaat gatgtgtggc tggatgctga aagccagcaa
    21661 gccgcgtgag caaaagcgac tacgtggttt tggtcgcatg ttggttgccc tgcaacaagg
    21721 ctacggcaag tcgctgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgttgct
    21781 tggcaccatt gcgctgaata tctggctgta tatctcgatc ccgaaaacct tcttcccgga
    21841 gcaggacacc ggcgtgttga tgggcgggat acaggcggat cagagtattt cgtttcaggc
    21901 gatgcgcgga aaattgcagg atttcatgaa aattatccgt gacgatccgg cagtggataa
    21961 tgtcaccggc tttaccggcg gttcgcgggt gaacagcggg atgatgttta tcaccctcaa
    22021 gccacgcggc gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc gggtgaaact
    22081 ggcgaaagaa ccgggggcga atctgttcct gatggcggta caggatattc gcgttggcgg
    22141 acgtcagtcg aacgccagtt accagtacac gttgttatcc gacgacctgg cggcactgcg
    22201 cgaatgggag ccgaaaatcc gcaaaaaact ggcaacgttg ccggaactgg cggacgtgaa
    22261 ctccgatcag caggataacg gcgcagaaat gaatctggtt tacgatcgcg acaccatggc
    22321 acggctggga atcgacgtgc aggccgccaa cagcctgtta aataacgcct tcggtcagcg
    22381 gcaaatctcg accatttacc agccgatgaa ccagtataaa gtggtgatgg aagtggatcc
    22441 gcgctatacc caggacatca gcgcactgga aaaaatgttc gtcattaata acgatggcaa
    22501 agcgatccca ctgtcgtatt tcgccaaatg gcaaccggcg aacgccccgc tgtcggtgaa
    22561 tcatcaggga ttatcggcag cctcgaccat ctctttcaac ctgccgaccg ggaaatcgct
    22621 ctcggacgcc agtgcggcaa tcgaccgcgc aatgacccag ctaggtgtac cttctaccgt
    22681 gcgcggcagt tttgccggaa cagcgcaggt gttccaggag acgatgaact cacaggtgat
    22741 cctgattatc gccgccatcg ccacggtgta tatcgtgctg ggtatccttt acgagagtta
    22801 cgtacatccg ctgacgattc tctccaccct gccctcggcg ggcgttggag cgctgctggc
    22861 gctggagctg ttcaatgccc cgttcagcct aatcgccctg atagggatca tgctattaat
    22921 cggcatcgtg aagaaaaacg ccattatgat ggtcgatttt gcgcttgaag cccaacggca
    22981 cggtaacctg acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat
    23041 tatgatgact accctggcgg cgctgtttgg tgcgctgccg ctggtattgt cgggcggcga
    23101 cggatcggag ttgcgccaac ccctggggat caccattgtc ggcggtctgg taatgagcca
    23161 gctcctgacg ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc tacggctacg
    23221 tttttcgcgt aaacctaaac aaacggtaac cgagtaaatg acagatcttt ccgacagcac
    23281 ccgctggcaa ttgtggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat
    23341 cgtaaacacc gcccttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca
    23401 catggtcatt gtctcttatg tactgaccgt ggcggtgatg ctgcccgcca gcggctggct
    23461 ggcggacaaa gtcggcgtgc gcaatatttt ctttaccgct atcgtgctgt ttactctcgg
    23521 ttcactgttt tgcgcgcttt ccggcacgct gaacgaattg ttgctggcac gcgcgttaca
    23581 gggcgttggc ggcgcgatga tggtgccggt cggaaggctt acggtgatga aaatcgtacc
    23641 gcgcgagcaa tatatggcgg cgatgacctt tgtcacgtta cccggtcagg tcggtccgct
    23701 gctcggtccg gcgctcggcg gcctgctggt ggagtacgcg tcgtggcact ggatcttttt
    23761 gatcaacatt ccggtgggga ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta
    23821 taccatgcag acgcggcgct ttgatctttc cggcttttta ttgctggcgg ttggcatggc
    23881 ggtattaacc ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc tggcgattgc
    23941 tggcctggtc gcagttggcg tggtggcact ggtgctttat ctgctgcacg ccagaaataa
    24001 caaccgtgcc ctgttcagtc tgaaactgtt ccgtactcgt accttttcgc tgggcctgac
    24061 ggggagcttt gccggacgta tcggtagcgg catgttgccc tttatgacac cagttttcct
    24121 gcaaattggc cttggcttct caccgtttca tgccggactg atgatgatcc cgatggtgct
    24181 tggcagcatg ggaatgaagc gaattgtggt acaggtggtg aatcgctttg gttatcgtcg
    24241 ggtactggta gcgaccacgc tgggtctgtc gctggtcacc ctgttgttta tgactaccgc
    24301 tctgttgggc tggtactacg ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc
    24361 gacccgtttc tcctccatga acaccctgac gctgaaagat ctcccggaca acctggcgag
    24421 cagcggcaac agcctgctgt cgatgattat gcaattgtcg atgagtatcg gcgtcaccat
    24481 cgccgggctg ttgctgggac tttttggttc gcagcatgtc agcgtcgaca gcggcaccac
    24541 acaaaccgtc tttatgtaca cctggcttag catggcgttt atcatcgccc ttccggcgtt
    24601 catctttgcc agagtgccga acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag
    24661 gagcgcgcaa tgaagttctg gcgacccggt attaccggca aactgtttct ggcgattttc
    24721 gccacctgca ttgtcttgct gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt
    24781 ggctttattg attacatcaa gcatggtaat gaacagcgat tacaactgtt aagtgatgcg
    24841 cttggcgagc agtatgcgca gcatggcaac tggcgcttcc tgcgcaacaa tgatcgcttt
    24901 gtctttcaga tcctgcgttc atttgaacac gataattcgg aagataaacc cggcccgggt
    24961 atgccaccgc acggctggcg tacccagttc tgggtggttg atcaaaacaa caaagtgctg
    25021 gttggtccgc gagcgccgat tccacctgac ggtacacggc gacccattct ggtcaacggt
    25081 gcggaagttg gcgcggtgat cgcctccccc gttgagcggt taacgcgcaa tactgatatc
    25141 aatttcgata aacaacagcg gcaaaccagc tggttgattg tcgctctggc aacgttactc
    25201 gcggcgctcg ccacctttct gctggcgcgc ggtcttttgg ccccggtcaa acggctggtg
    25261 gacggcacgc acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa
    25321 gatgaactgg gcaaactggc gcaagacttc aaccagcttg ccagcacgct ggagaaaaac
    25381 cagcaaatgc ggcgcgattt tatggccgat atttctcacg aactgcgtac gccattagcg
    25441 gtgctgcgcg gtgaactgga agccattcag gatggcgtgc gtaaattcac gccggagacg
    25501 gtggcgtctt tacaggcgga ggtcggtaca ctgaccaaac tggttgacga tctccatcag
    25561 ttgtcgatgt ctgatgaagg cgctctcgcc tatcaaaaag caccggtaga tttgatccca
    25621 ctgctggaag tggcgggagg cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg
    25681 caattttccc tgccagacag tattaccgta tttggcgatc gcgaccgttt aatgcagtta
    25741 ttcaataact tacttgaaaa cagcctgcgc tacactgaca gcggcggcag cctgaaaatc
    25801 tctgccgagc aacacgacaa aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc
    25861 agtgacgatc agctacaaaa attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac
    25921 cgtgccagcg gcggttccgg gctggggctg gcgatttgcc tgaacattgt tgaagcacat
    25981 aatggccgca ttattgctgc ccattcgcct tttggcgggg taagcattac agtagagtta
    26041 ccgctggaac gggatttaca gagagaagta tgaccgagtt accaatcgac gaaaacacac
    26101 cgcgtatttt gatcgtggaa gatgaaccga agctgggaca gttgctcatt gattatctgc
    26161 gtgctgcgag ctatgcgccg acgcttatca gccacggcga tcaggtactg tcgtacgtgc
    26221 gccagacgcc gccggatctg atcctgttgg atctgatgct ccctggcacc gatggcctga
    26281 cgctgtgccg ggaaattcgt cgtttttctg acattcctat cgtgatggtg acagcaaaaa
    26341 tcgaagagat cgaccgcctg ctagggctgg agattggcgc agatgactac atttgcaagc
    26401 cgtacagccc acgggaagtg gtagcgcgtg tcaaaaccat tttgcgccgt tgcaaaccgc
    26461 agcgtgagtt gcagcaacag gatgctgaaa gcccgttgat tatcgacgaa ggtcgttttc
    26521 aggcttcatg gcgtggcaaa ttgcttgacc tgacgcctgc ggaatttcgt ctgctgaaaa
    26581 cgctctctca tgaaccagga aaagtgttct cccgcgagca attgctcaat catctttatg
    26641 acgactaccg cgtagtaacc gaccgcacca tcgacagcca cattaaaaac ctgcgccgca
    26701 agctggaatc tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc gttggttacc
    26761 gctgggaagc cgacgcctgc cgcatcgttt agttttagcg acattacttt gttagccgga
    26821 gatggcgttc agaccagatc cggcaacatt atcccacgca tggtcagcaa actgacatta
    26881 tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcggtaa acagcacctt
    26941 tagatgctgt tttcgatacg caatttcaat caaggagtca ttatggctgg ttggtttgaa
    27001 ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa tggtgagacg
    27061 atcctcacca gcgagctgta tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg
    27121 cgtagcaaca gcccgcaaga agaacgctat gagaaaaaaa cggcaagaaa cggcaaattc
    27181 tatttcaatc tgaaagccgc caatcatcaa attatcggct ccagccagat gtacgccacc
    27241 gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag tcagacggta
    27301 aaagacaata cgtaattacg atgccgggcg tgggttgttg gcgtccggct ttatttccaa
    27361 ataacgctta tttatttacc tgcgccatta aaatagtgga cactgatgaa tactcattgt
    27421 gctttctttg tttaacatct ccatgcttat cctcctgaac atgttcatct gccaaaatat
    27481 cttctaatac tttcgtgacg ctatccggtt tatgacatac actgatgtaa gatttttact
    27541 tacaggagag cataaacatg gccagaacga tgacggttga tctcggcgat gagttgcgtg
    27601 aatttatcga gtccttaatt gaatccggcg actatcgcac ccaaagcgaa gtgatccgtg
    27661 agtcactgcg cttacttcga gaaaagcaag cagagtctcg cctgcaagcg ttacgtgaca
    27721 tgcttgcaga agggttgagt agtggtgaag ctcagccgtg ggaaaaggac gcgtttttac
    27781 ggaaggtgaa agcagggata aggaaataat gtgcgtatta ttaaactcat gccaaaagcc
    27841 aatgaggatt tagaaggcat ctggtattac agttatcacc atttcggcga accacaagcc
    27901 gacagatatg tggaacatct ttcagacgtt ctccagattc tgagtaacaa taatataggt
    27961 acgcccagac ctgaattagg cgaaggtatt tttgttcttc cttttgaacg ccacgtaatt
    28021 tatttcctgc aatcaccagg ggaaattatc gtcattcgta tccttaacca aaaccaggac
    28081 gccaccagac atttacactg gagctaaacc cgccgattgc gctacaatgc ccgcccttaa
    28141 agtgggggca ctcccctaac cgcttcatca ggtgaagcgg atctgacctg tcatcagaac
    28201 gagagaatta tgtttaaacc ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt
    28261 tacgctttcg cttatggcgc agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt
    28321 gtgcgcaaca acgaattcaa ccacgaaaat cttcagctcg gcatcaatga agcccacgcg
    28381 ctggggaaaa agttttatgt cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc
    28441 tttatccgtg acctgaaacc ggtggtggaa atggggccgg atgcgctgat tatgtccgat
    28501 ccagggctga ttatgctggt gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg
    28561 caggctaacg ccgtaaactg ggcgacggtg aaattctggc agcaaatggg cctgacccgc
    28621 gtgatcctct ctcgcgagct gtcgctggaa gagattgaag agatccgtaa tcaggtgccg
    28681 gatatggaga tcgagatctt cgttcacggc gcactgtgca tggcctactc cggtcgctgc
    28741 ctgctctctg gctatatcaa caagcgcgac ccgaaccagg gtacctgcac caacgcctgc
    28801 cgctgggagt acaacgtcca ggaagggaaa gaagatgaag ttggcaacat cgtacacaag
    28861 tacgagccga ttccggtgca aaatgttgag ccgacactgg gtatcggcgc gccaaccgac
    28921 aaagtgttta tgatcgaaga ggcccagcgt ccgggcgagt atatgaccgc gtttgaagat
    28981 gagcacggca cttacatcat gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc
    29041 cttaccaaaa tgggcgtgca ttcgctgaaa atcgaaggtc gtaccaaatc tttctactat
    29101 tgcgcacgca ccgcacaggt ttaccgcaaa gctatcgatg atgccgctgc gggcaaaccg
    29161 tttgacacca gcctgctgga aaccctggaa ggtctggcgc atcgtggcta taccgaaggt
    29221 ttcctgcgtc gtcatactca cgacgattat cagaactacg aatacggtta ttcggtttct
    29281 gaccgccagc agtttgttgg tgagtttacc ggtgagcgca agggggacct cgcggcggta
    29341 gcggtgaaaa ataaattctc cgttggcgac agccttgagc tgatgacgcc gcaaggcaac
    29401 attaacttta cccttgagca catggaaaac gccaaaggcg aagctatgcc ggtagcacca
    29461 ggcgatggtt atactgtgtg gctcccggtg ccgcaggatc ttgagctaaa ttacgcgctg
    29521 ctgatgcgta atttctccgg ggaaaccacg cgtaacccac acggtaagtg attaatttcg
    29581 attatttttc ccggatggaa aattcttaga aaccgatcac atacagctgc atttattaag
    29641 gttatcatcc gtttcgctga aaaacataac ccataaaatg ctagctgtac caggaaccac
    29701 ctccttagcc tgtgtaatct cccttacacg ggcttatttt ttatgcataa gccctatccc
    29761 tggtcaccgt cttccattga ccacatcgat agaatctccc ttcatagcac gatgcctttc
    29821 acgtaacggc atcgtgctcg cacaggttcc ggctaagcac aaccagaacg cgcatgtttg
    29881 acgcttacca aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
    29941 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
    30001 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggataaa
    30061 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
    30121 gcaaggccaa atttaccgat acttgtctca tcattatgca cagcacaacc agacataaga
    30181 aaaacactaa ttaatgatat agcagctatc ttgttcacct caccttcccc cattaa
//
LOCUS       DAPGRN010000057        29909 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPGRN010000057 DAPGRN010000000
VERSION     DAPGRN010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.057"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..1915
                     /locus_tag="RHW33_002624"
     CDS             131..1915
                     /locus_tag="RHW33_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV1646112.1"
                     /translation="MNTVKLSLLAVVVATAVSPSAFAGDTVEAAATELAAIQPGMSQV
                     EIDQKIGRFLERTGNSAAAQNYLIAHDYQTTTPQENTTASPVQPASTLNPITNQAQTV
                     RDNGQDTAIQDAQHAADWASQKADDAHHAIMVAQTDIDANKAAITDTRNDVSAVQSDV
                     TNIKADVAHAQSTADHANANANTALMNGVKLSGAVTENKNNIEQNRSDIADQQKRLAS
                     NEHKQTVRDNEQDTAIQDAQHAANWASLKADDAQHAITVAQTDIDANKAAIADTRNDV
                     SAVQSDVTNIKADVTHAQSTADHANANANTALINGVKLSGAVTENKNNIEQNRSDIAD
                     QQQQLDETRKTVAATGDVQTAARYQSMIDARQTAAANAQQEQADTQQQQMDDQQKQID
                     ATQKTVSALGDAQTNAHYQEMVNAGLRAQNDANARTAAEQKQKIDTLATNQATQQHID
                     SVQYGEQIQRLAQDSTQTHEQIDSLTQDVTQTHQQLSNTQKRVADNSQQITTLNNHFS
                     SLKNEVEDNRKEANAGTASAIAIASQPQVKTGDLMMVSAGAGTFNGESAVSVGTSFNA
                     GTHTVIKAGVSADTQSDFGAGVGVGYSF"
     gene            2001..2423
                     /locus_tag="RHW33_002625"
     CDS             2001..2423
                     /locus_tag="RHW33_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646113.1"
                     /translation="MMNSSIKSFSLLAVILLAGCSSPTSRIADCQAQGVSHDTCYLAE
                     QQRQTAILSASEAQAFKNAEAAQHAQAAKKAIYKGFGVTFRMSSKNFAYLNDSLCAID
                     EDNKDATVYQSGLYNVIVYHHTGKVALMKEGQFVGYLK"
     gene            2715..3938
                     /locus_tag="RHW33_002626"
     CDS             2715..3938
                     /locus_tag="RHW33_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646114.1"
                     /translation="MLFPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVLYDPHPYGL
                     IRSLCWQDVPVIRFWSEAGCPTCAEFVYSGFADDEQGAARFLSALENWNRPWSGFTDA
                     FAALTPLFSCLADGYYLLEDRELYPTDGNGHFFWAATDHSASNPATVAVWDPEYCYFC
                     NTAPCFLLPGQPPSHFNPERVTFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATRFNDEQQTLVFPGGECLHKTELLCHVPKLTEWKTLPSGAWESFGP
                     DKHISPSETWSEELQQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADVI
                     LQALFFTHSPLFIDFARFVISYPAYVSYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKR"
     gene            complement(3939..4346)
                     /gene="cueR"
                     /locus_tag="RHW33_002627"
     CDS             complement(3939..4346)
                     /gene="cueR"
                     /locus_tag="RHW33_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV1646115.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(4343..5518)
                     /locus_tag="RHW33_002628"
     CDS             complement(4343..5518)
                     /locus_tag="RHW33_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDV1646116.1"
                     /translation="MNGQLQYNRGLTEPRLPRSALAVRVTAVMLLCFLGWAWYFQLDE
                     VTTGSGTVEPSGREQVVQSLEGGILYHLNVKVGDIVEQGQPLAQLNRTKTESDVQEAM
                     SRLYAALATSARLRAEVSNKPLVFPDELNKFPELIESETALYNTRRDGLNKATTGLTQ
                     GISLVNRELAMTQPLVKQGAASSVEVLRLQRQANELENKLSDVRTQYYVQAREELAKA
                     NAEVETQRSVIRGREDSLTRLNFTAPVRGIVQDIDVTTVGGVIAPGGKLMTIVPLDEQ
                     LLIEAKISPRDVAFIHPGQKSLVKITAYDYSIYGGLPGEVAVISPDTVQDEVRRDVYY
                     YRVYIRTFSNHLENKSKQQFPIFPGMVATVDIRTGKKSVLDYLLKPFNKAQEALRER"
     gene            complement(5515..7677)
                     /locus_tag="RHW33_002629"
     CDS             complement(5515..7677)
                     /locus_tag="RHW33_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDV1646117.1"
                     /translation="MKKNAQSVEAWLEAMIAVARYYRLDFSQENVRATVNWERGSKRE
                     ELLTDMARQLGMGLRLVEFSADSLNPWRLPLIAVFDNQQIGVITRRDNHDNISVQFSG
                     DEGLETTLNVADVEDKIVELALLRPLSAIPDARVDDYIRPYQANWFWNLSLKDWRRYG
                     DIMLASLVANVLALAAMIFSMQVYDRVVPAQSYPTLWVLFAGVMMAILFEFCMRMVRT
                     HLSDVIGKRADLRISDRVFGHALRLKNNVRSKSTGSFISQIRELESVRELITSTTIGA
                     VADLPFFLLFVFILWMIGGWLVLVVLLALPLLVIPGLLVQRPLARLANEGMRESAVRN
                     ATLVEAVQSIEDIKLLRAEQRFQNQWNHTNDVASSISMKQRFLTGLLLTWTQEVQSIV
                     YVVVLLVGCFMVMNGDMTTGALVGTTILASRTIAPLSQISGVLSRWQQAKVARNGLDE
                     LMKRPVDQPEHGKLVHKAVLHGNYQFSNAIFYYDEEEKIADVAIGKLHIQAGEKIAIL
                     GRNGAGKSTLLQMLAGMRIAQQGQVLLDNISIGQLDPADLRRDMGLLSQTGRLFFGSL
                     RENLTMGMPEASDEDIERALTLSGALPFVQKQKNGLNYMIQEGGFGLSGGQRQTLLLA
                     RLLISQPNIVLLDEPSASLDEMAEAHLIEQLKQWIGHRTLIIATHRTAMLQLVDRIIV
                     MDQGRIVMDGAKEAILREQGEPTARRVVLQEKNKGSAA"
     gene            complement(7681..24963)
                     /locus_tag="RHW33_002630"
     CDS             complement(7681..24963)
                     /locus_tag="RHW33_002630"
                     /inference="COORDINATES: protein motif:HMM:NF033510.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV1646118.1"
                     /translation="MDLTPAAITINTIATDDVINAAEKGANLTLSGTTTNVEAGQTVT
                     VTFGGKNYTASVAGDGSWTATVPAADLAALPEGSASAQASVSNINGNSASAVHSYSVD
                     SSAPTIIINTVASDNIVNASEADAGVTVSGSTTAEAGQIVTVTLNSPTVQTYQATVQA
                     DGSWSINIPAADLAALTDGSHTLMATVNDKAGNPASTTHNLAVDLTVPVLTINTIAGD
                     DIINATEHGQALVISGSSTGGEAGDVVSVTLNSKTYTTTLDASGNWSVGVPAADVTAL
                     GSGPQTVTATVTDAAGNSDSETHTVTVNLTAPTIGINTIATDDVINATEKGADLQISG
                     TSNQPAGTTITVTLNGQNYTATTDASGNWSTTVPASAVGALGEASYTVTANVTDSAGN
                     SNSASHNVQVNTALPGVTINPVASDDIINAAESGVAQTISGQVTGAAAGDTVTVTLGG
                     KTYTATVQGNLSWSVDVPAADIQAIGNGDLTVNASVTNGVGNTGSGSRDITIDANLPG
                     LRVDTVAGDDVVNSIEHAQALVITGSSSGLAAGAALTVVINTVTYAATVLADGTWSVG
                     VPAADVGNWPAGTVNIIVSGTNTAETTTTITHPVTVDLAAVAISINTVSGDDVINAAE
                     KSADLTLSGSTSGVEVGQTVTVTFGGKTYTATVAGDGSWTTTVPAADLSALRDGDATV
                     QASVSTINGNTASATHAYSVDATAPTLAINTIATDDILNAAEAGNPLTISGSSTAEAG
                     QTVTVTLNGVTYSGTVQADGSWSVSVPTADLSNLTASQYTVSASVSDKAGNPASATHG
                     LAVDLTVPVLTINTVSGDDIINATEHGQALVISGSSTGGEAGDVITVTLNSKTYTTTL
                     DASGNWSVGVPAADVTALGSGPQIITAAITDAAGNSDDASRTVTINLTAPTIGINTIA
                     SDDVINATEKGADLQITGTSNQPAGTTITVTLNGQNYTATTDSNGNWSATVPASAVSA
                     LGEANYTVTASVTDTAGNSNSASHNVLVNSALPAVTINAVATDDIINAAEAGNAQTIS
                     GQVTGAAQGDTVTITLGGNTYTATVQANLSWSVSVPAADIQALGNGSLTVNASVTNGV
                     GNTGSGSRDITIDANLPGLRVDTVAGDDVINSIEHNQALVITGSSTGLTAGTALTVEI
                     NNVTYAATVLADGTWNLGVPAADVSNWPAGTVDITVSGTNSAGTTSTITHPVTVDLAA
                     VAITINTLSGDDVINAVEKGETLVVSGSTSGVEAGQTVAVTFGGKNYTTTVEANGSWT
                     VNVPPADLAALPDGAGNVQASVSNINGNNAQADRAYSVDATAPLVTINTIASDDTLNV
                     SEAGAGITISGTTTAQAGQTLTVTLNNNTYQTTVQADGTWSVNVPAADLSGLTASSYT
                     VTATVSDKAGNPASADHALAVDVTAPDLTINTVAGDDIINAIEHGQALVVSGTSTGAA
                     AGDVVTVNLNGKNYTTTLDASGNWSVGIPAADVTALATGSQTITASLSDRAGNSDSTT
                     HNVTVDLSGPTLTISIVSGDDIINNAEKTQDLTISGGSSGLATGTTVTVMLNGLAYSA
                     TTDSSGNWSVTVPASAVGALGEAVYQISASATDSAGNSGSTTHTVNVESLLPGVIINT
                     VAGDDIINAAEIAVAQTISGQVTGTAAAGNTVTVTIGGNQYTATVQPDLSWSVSVPAN
                     VLQALGNGELTISASVTNSANNTGTATHDIVIDANLPGLRVDTVAGDDVINSIEHTQA
                     LVITGSSSGLAAGAALTVVINSVTYGATVLADGSWSVGVPAADVTNWPEGTVNIAVSG
                     TNTAGTTTSITHPVTVDLAAVAITINPLSTDDVINAAEKGSDLQLSGTTSDVEAGQTI
                     TVIFGGKSYTTTVAADNTWGLTIPAADVATLPDGAANVQASVSNVAGNSAQATHAYSV
                     DATAPSVTINTIATDDILNAAEAGSALTISGTSTAEAGQTVTVTLNGVNYSGNVQADG
                     SWSVSVPTGDLASLTASSYTVNASVSDKAGNPASATHNLTVDLAAPVVTINTVAGDDI
                     INATEHGQAQIISGSATGATTGNTVSVTIGTTTYTTVLDANGNWSIGVPASVISALAQ
                     GDVNISATVTDSAGNSGTASHVVSVALGAPVLAINTIAVDDIINATEKGADLAISGTS
                     DQPAGTQITVTLNGQHYTTTADASGNWSVTVPASAVGTLGEATYTVTASATDADGNSG
                     SASHNVQVNTTLPGVTINVVATDDIINAAEAGVDQTISGQVTGAAAGDTVTVTLGGAT
                     YTATVQANLSWSVDVPASALQALGNGELTISASVTNSVGNTGNGTREITIDANLPGLR
                     VDTVAGDDVVNIIEHNQALVITGSSSDLAAGSNVTLTINGQTYVAAVLADGSWSVGVP
                     AADVSAWPAGKVTITASGNTTAGNPVSVTHPVTVDLTAVAVSINAITADDVINAAEKG
                     AALTLSGSTSGVEAGQTVTITFGGRTYSATVAANGSWSTSVPAADMAALRDGDASAQA
                     SVSNVNGNSATTTHAYSVDASAPTVTINTIAGDDILNAAEAGAALTITGSSTAEAGQT
                     VTVTLNGENYTGTVQTDGSWSVSVPQADVSALTASNYTVSAAVNDKAGNPASVNHNLT
                     VDTSVPVVTINTVAGDDVINATEHAQAQIISGSATGAATGSTVTVTIGTNTFTTVLDA
                     SGNWSVGVPASVVSALANGTVTINASVTDAGGNSGSTTHQVTVNTGLPTITFNTISGD
                     NVLNADEKGQPLTISGGSTGLATGAQVTVTLNGHNYSATTDASGNWTLTVPVSDLAAL
                     GQANYTVSASATSAAGNTASSQANLLVDSGLPAVTINTVAGDDIINAAEAGANQTISG
                     VVTRAAAGDTVTVTLGGNTYTTTVQGNLSWSVSVPAADLQALGNGDLTIIASVTNGNG
                     NTGSGTRDITIDANLPGLRVDTVAGDDIVNSIEHGQALVITGGSSGLNAGVPLTITIN
                     GTTYSATVQADGSWSVGIPAANVSAWPAGPLAVDVTGQSSAGNPVSVSHPFTVDLTAV
                     AISINTVASDDVINAAEKGTDLTLSGSTSGIESGQTVTVTFGGKTYTASVAANGSWSI
                     TVPAADLATLPDGAANVQASVSSASGNSASATHAYSVDASAPTLTINTIASDDILNAT
                     EAGNPLTISGTSTAETGQTVTVTLNGATYSGNVQADGSWRVSVPTSALGALTASNYTV
                     SATVNDKAGNPGSASHNLAVDTTAPVLTINTVAGDDIINDAEHAQALVISGTSTGGEA
                     GDVVSVVINGKTYTTTLDASGNWSVGVPAADVTALGSGAQTITASVSDRAGNSDDASR
                     TVTVSLSAPVISINTIAGDDVINATEKGSDLALSGTSDQPAGTAIIVTLNGQNYSATT
                     DASGNWSVTVPASAVSALGEATYSVTASVTNAQGNSSTASHNVQVNTALPGITINPVA
                     TDDIINAAEAGSAQTISGQVTGAAAGSTVTVELGGKTYTATVQADLSWNVSVPAADWQ
                     ALGNGELTVNASVTNAVGNTGSGTRDITIDASLPGLRVDTVAGDDVVNIIEHAQAQVI
                     TGSSSGFAAGTALTVVINNQTYAATVLANGTWSVGVPATDVSNWPAGTLNITVSGANS
                     AGTQTSITHPLTVDLTTVAVSMNSITSDDVINVAEKGAALTLSGSTSGVEAGQTVTVT
                     FGGKNYTTTVAANGSWSTTVPAADLAALRDGDASAQVRVTNVNGNSATATHEYSVDSA
                     APTVTINTLASDNIINASEAAAGVTVSGTSTAQTGQTLIVTLNGTNYQTTVQADGSWS
                     LTLPASDLTALANNGYTLTATVSDLAGNPGSASKGVTVDTTAPVISFNTVAGDDVINN
                     VEHTQAQIISGTATGAVAGDRLVVTIAGQQYVTSTDASGNWSVGVPASVISGLADGTV
                     TISATITDSAGNSSTQTHNVQVNTAAVSLSVSTISGDNLINAAEAGSALTLSGTGTNF
                     AAGTVVTVLLNGKGYSATIQSNGSWSVNVPAADVAALADGTSYTVSASAQDSAGNSAT
                     ASRSVTVDLTAPVISINTVSTDDRLNAAEQQQPLTLNGSTSAEVGQTVTVTFGGKTYT
                     ATVAANGTWALNVPAADLAALGQGAQTITASVNDRAGNPGQTTHALTVDTVAPTVTIA
                     TVAGDDIINNAEQLAGQTISGTTTAEVGQTVTVTFNGQSWTATVGSGGSWSVFIPAQQ
                     FAGLSDGSYTISATVSDQAGNPGSASRGVTLNGDVPTVTINTFAGDDVVNAAEHGSSL
                     VISGTTTAPVGQTLTLTLNGKTYTTTVQTGGSWSYTLGSADVTALADGNAYVINASVS
                     NAIGNTGSSNHTITVDLSAPAMGINIDSLQADTGLSASDFITSVSPVVVNGSLTAALA
                     SNETAQISIDGGTTWTTLTVTGTTWRYNDSRTLTDGNYLYQVRVIDAAGNVGATDSQN
                     VVIDTTAPDPAVKTIAISAITTDTGLITNDFVTSDTTLAVSGTLGAALSAGEFAQISI
                     DGGTTWQNLSVSGLTWTWLDGRTLTDGNYNYQVRVIDTAGNIGATASQIVTVDTTAPL
                     ASKTIAIAGISDDTGLSSSDFVTRDTTLTVRGTLGAALAADERAQISLDGGVTWTTLT
                     VVGTSWSYADGRTLTDGTWNYTVRVVDLAGNVGQTATQNVVVDTTSPEAAKSITITGI
                     SDDTGASSSDFITSDTTLTVRGVLGAALGANEFAQISTDNGATWVNVTLAADGLNWSY
                     VDGRTLTNGTTTWQVRVVDLAGNVGATGSQSAQIDTVNPAQVLTIASISTDTGSSATD
                     FITSDTTLTLTGSLGAGLASGEVAQISLDSGATWITLTTNGTQWTYTDSRTLTDGSYV
                     YQVRVLDLAGNTGPVVSKTVVVDTINPTATPTIVSYTDDVGQRQGTLSSSQATDDTTP
                     LLNGVLSAPLASGEVVYLYRNGLLLGAVTMVGALNWTYSDSGLVSGAYTYSARVVDLA
                     GNITSSSDFVLTVDTSIPTTLAQITSQTTRDTTPIISGVITAALASGQYVEVVINGKT
                     YTSEPGGAVVVDPAHNTWYVQLPDTDALTVSATAYTVTAQVKSSAGNGNNANISNGTV
                     TVNAAIDYTPTWTTASKTTAWGLTYGLDSHGMWTVLANQQVMQSTDPLTWSKTALTLY
                     QSGNNYATSSIADYDRNGTGDLFITRDDYGTGYINGFTNNGDGTFSSAIQVTVGTLTW
                     YGSIVAFDKEGDGYLDFWIGDAGGPDSNTFLWNNAGTLVGNSTTSNSGGSATVGGAVT
                     GYLSLNEGFGVDLNNDGRIDLIQHTYNLNNYYTLSSLINQGNGTFVWGQNTTNTFLSG
                     TGSGAMSSSVSMTWADFDGDGDMDLFLPASQGRANYGSLLFNTNGVLGSPVAVGATAT
                     TYASQFSLAVDWNHDGLMDIARIAQTGQSYLYTNVGGASNWTQSALGGSQSGTTSDVA
                     AMDYDWDGAVDVLVTKQSGSVFLIRNTNTVSYGTSLHLRITDPNGINVYYGNTVKLYN
                     SAGVLVATQIINPQSGMGVNDTSALVNFYGLNAGETYNAVLIKSTGTTASNIDQTVNT
                     SWGGLQATDATHAYDLSAEAGTASNNGKFVGTGYNDTFFATAGTDTYDGSGGWVYSSG
                     TGTWLANGGMDVVDFRLSTVGVTANLSSTAAQATGFNTSTFTNIEGISGSNFNDTLTG
                     SSGDNQLEGRGGNDTLNIGNGGHDTLLYKLLNASDATGGNGSDVVNGFTVGTWEGTAD
                     TDRIDIRELLQGSGYTGNGKASYVNGVATLDAQAGNIGDFVKVTQSGSDTIVQIDRDG
                     TGGTFATTNVVTLTGVHTDLATLLANHQLMVV"
     gene            complement(24984..28457)
                     /locus_tag="RHW33_002631"
     CDS             complement(24984..28457)
                     /locus_tag="RHW33_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV1646119.1"
                     /translation="MSLIIDVISRKTSVKQTLINPGDVTVVIYEPSVVQVHAQASAVA
                     RYVREGNDLLIYMQDGTVIRCNGYFLQAANTAEQSELVFADGQQLTHVTFADTAAGGL
                     APVELTAQTTAIESIAPFLDTVAQTSAFPWGWLAGAAVGGGALGALLASGGDGDSKTE
                     VINNPTPPAEPGNATPSFLVTDNQGDQRGILATNDITDDTTPTFSGSGQAGATIQIKD
                     SNGNTIASTQVDNNGHWSVSLPTQSAGEHTWSVVQIVGSTITDAGSITLTIDNSQASV
                     QVATTAGDNIINASEQAAGFTLSGTSSHLAQGTELTVTLNGKTYTTSVGANGAWSVQV
                     PTADAQALGEGNQAVLVSGKDTTGNTVTGAQLLTVDTQPPTLAINTIAQDNIISAAEH
                     NVALVLSGTSNAEAGQTVTLTVNGKSHTATVGSDGTWQVTLPATEVQALAEGNYAVNA
                     SVSDRAGNTTSHSANFTVDTSAPVVSVNTVAGDDILNNAEQAVAQIISGQVSGASPGD
                     TVTVKLGTHVLTGIVLADGSWNVALDPAVTRTLDRGANTIFVTVTDAAGNTGAASRAI
                     TLVGVSPLITINTVSGDDIISGAEKGAPLTLTGSTQQAETGQTVTVTLAGQSFTTTVQ
                     ADGSWSLTVPAAAMGNLPDGAVAITASVTDLSGNTGNTSRTITVDSQAPALSIDPLTA
                     DNIINAAESGQDLPITGTTDAQPGQTVTVTLNGQTYQGVVQPDGTWSVTVPAANVDAL
                     ADGNATVTASVNDVAGNPTSVSRVALVDATPPVVTINPVATDNVINTPEHTQAQIISG
                     TVTGAQAGDIVTVTLNNMDYTTVVDGSGNWSLGVPASVVSGLADGSYPVSVSVTDKAG
                     NTGSQSLTVTVNTAAPLIGINSIAGDDVINASEKGADVQITGTSDQPVNTAITVTLNG
                     QNYTATTDASGNWSVTVPASAVTALGQANYTVTVAVTSSIGNSATASHNVLVDSALPG
                     VTINPVATDDIINAAEAGVAQTISGQVTGAAVGDAVTVTLGGNTYTTTVQAGLSWSVD
                     VPADAIQALGNGDLTVNASVTNQNGNTGSGTRDITIDANLPGLRVDTVAGDDVVNIIE
                     HGQALVVTGSSSGLAEGTPLTVTINDVEYTTAVQADGSWSVGITAAQVSAWPAGRLIL
                     PYPGKVAPEIR"
     gene            complement(28566..29861)
                     /locus_tag="RHW33_002632"
     CDS             complement(28566..29861)
                     /locus_tag="RHW33_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HDV1646120.1"
                     /translation="MVMGEAAIAAPLPQWANAPAVTPVAQLSLQESILRAFARNPDVT
                     QQAAQIGIGEAQIDEAKSAWYPHVGLTGNAGPSRQTDSSGRLDNNVSYGITLTQLVYD
                     FGKTNNDINLQTAARDSYRFKLMATLTDVAEKTATAYMEVSRYQALCDAAQRNIHSLE
                     NVYNMAALRANAGLNSSSDELQAQTRIAGMRSTLEQYQAQMASAKAQLAVLTGVQPEA
                     IAAPPAELAEQPVSLKNIDYQSIPLVLAAENLRQSAQYGVEKTKAQYWPTLSIQGGKT
                     RYQTSDRSYWNDQLQLNVNAPLYQGGAVSAQVQQAEGQQKISASQVEQAKLDVLQRAS
                     VAYANWTGARGREEAGLAQSESAHKTRDVYQNEYKLGKRSLNDLLTVEQDVFQAQSAE
                     INANYDGWVAAVNYAAAVNNLIPLAGIKQGLYNDLPDLK"
BASE COUNT         5697 a         8765 c         8636 g         6811 t
ORIGIN      
        1 aatgcgcttt acatcacaca aatggcggcg tagatttcga tcaaattgca acgcagatta
       61 attcttataa caacgtttta tgttgcttat agaaacaaat atgtgactta ctttggacga
      121 gaaaaaatac atgaatactg taaagttgtc tttactggct gtcgttgttg ctaccgcagt
      181 aagtccatct gcgtttgcgg gtgatactgt tgaggcggca gcgacagaat tagcggcaat
      241 ccagccagga atgtcgcaag tggaaattga tcagaaaatt ggtcgatttt tagaaaggac
      301 aggaaatagc gcagccgcac aaaattatct gattgcgcat gattaccaga caacgacgcc
      361 tcaggaaaat acaactgctt ctcccgtaca gcccgccagt acgctgaacc cgataaccaa
      421 tcaagcgcag accgtccgcg acaacgggca ggacaccgcc attcaggacg cgcaacatgc
      481 cgccgactgg gcgtcgcaaa aagctgatga tgcgcaccat gccattatgg tggcgcagac
      541 ggatattgat gccaataaag ccgccatcac cgacacccgc aatgatgtct ccgcagtgca
      601 gtcagacgtc accaacataa aagccgatgt cgcacatgct cagtcaacgg ctgaccatgc
      661 caacgctaac gccaacaccg ctctgatgaa cggcgtcaaa ctttccggtg ctgtgacaga
      721 aaacaaaaac aatatcgaac agaaccgcag cgatattgct gaccagcaga aacggctggc
      781 atcaaacgag cacaaacaga ccgtccgcga caacgagcag gacaccgcca ttcaggacgc
      841 acaacatgcc gccaactggg cttcactgaa agctgatgac gcgcagcacg ccatcacggt
      901 ggcgcagacg gatattgatg ccaataaagc cgccatcgcc gacacccgta atgatgtctc
      961 cgcagtgcag tcagacgtca ccaacataaa agccgatgtc acacatgccc agtcaacggc
     1021 tgaccatgct aacgctaacg ccaacaccgc tctgattaac ggcgtcaaac tttccggtgc
     1081 tgtgacagaa aacaaaaata atatcgaaca gaaccgcagc gatattgctg accaacagca
     1141 acaactcgac gaaacccgga aaaccgttgc cgccaccggg gatgtgcaga ctgccgcccg
     1201 atatcagagc atgattgacg ccagacagac agccgctgcc aatgcgcaac aggaacaagc
     1261 ggacacccag cagcaacaaa tggacgatca gcagaaacaa atcgacgcga cgcaaaaaac
     1321 ggtttccgca cttggcgatg cccagaccaa cgcacattat caagagatgg ttaacgccgg
     1381 actgagagca caaaatgatg cgaatgcgcg tactgcagca gaacaaaaac aaaaaataga
     1441 tactctggcg actaaccagg caacgcaaca gcatatcgat agtgtgcagt acggggaaca
     1501 aattcagcgt ctggcgcaag actcaacgca aacgcatgaa caaattgaca gcctgacaca
     1561 agacgtaacc caaacgcatc agcagttaag caacacgcaa aaacgagtag cggataatag
     1621 ccaacagatt actacgctca ataaccattt cagttcgctg aaaaacgaag ttgaggataa
     1681 tcgtaaagaa gccaatgcgg gaactgcatc tgccatcgct atcgcctcac aaccccaggt
     1741 gaaaaccggt gacttgatga tggtgtcagc gggagcggga accttcaacg gtgaatctgc
     1801 ggtgtctgta ggaacatctt ttaatgccgg aacgcatacc gtgattaaag ccggtgtctc
     1861 tgcggataca caatctgatt tcggcgcggg tgtcggcgtg ggatattcgt tctaatattt
     1921 caatcctcaa tataaataag agcaaggaag cttgccggtt ttcacctctt cattaatttg
     1981 tacattattt aaggttaaca atgatgaata gctccattaa atcgttttcc ctgctggcgg
     2041 ttatattact ggctggctgt agttcaccca cttcccgcat cgcagattgc caggcgcagg
     2101 gcgtcagcca tgacacctgt tatctcgcag aacagcagcg ccagacggct attttaagtg
     2161 catcagaagc acaggcattt aaaaatgcag aagccgcaca acatgctcag gcggcaaaga
     2221 aagccattta taaaggattt ggcgtcacct tcagaatgag cagtaagaac tttgcttatc
     2281 tcaatgattc attatgtgca attgatgaag acaataaaga tgccactgtt tatcagtcag
     2341 gtctgtataa cgtcattgtt tatcatcaca caggaaaagt cgccttaatg aaagaaggcc
     2401 agtttgtggg ttatttaaaa tgaaggagca aaggaaaata cccctgacgc atattatgat
     2461 tatcggtgcg tttatttttg ccttcttgca agtagtatta ttagcctccc tggttcacgc
     2521 tgtgaatgtt aacaatgaaa tccaggaagg cttatttcaa tcggggcgca taatggtaga
     2581 aagtttgcag catattcttt cggtgcaaac ggggattaat gggtaaggat accgttacca
     2641 tttccagagc aatagtcacc agcgtaattt tctggcacag ttcagggttc tgaatattcg
     2701 gctaaggagt ggccatgctt tttccctttc gaaaacctga agtacgtctg ctgaaaaata
     2761 ccgctgcggg cgtcattttt tcggtgaagt ccggtaaagg atttctgcgt caatgtgtgc
     2821 tttacgatcc gcacccttat gggcttatcc gttcactctg ctggcaggat gtcccggtaa
     2881 tacgtttctg gtccgaggcc ggctgcccca cctgcgcgga atttgtctac agcggttttg
     2941 cggatgatga acagggagcc gcccggtttt tgtctgctct cgaaaactgg aatcgcccat
     3001 ggtctggctt tacggacgct tttgccgcgc ttacaccgct gttctcatgc cttgctgatg
     3061 gctattatct gctggaagat cgtgaacttt acccgacgga tggcaatggg catttctttt
     3121 gggcggcaac ggaccatagc gccagtaatc cggcaaccgt tgccgtctgg gatcctgaat
     3181 attgctattt ctgtaacacc gccccctgtt ttcttcttcc tggacagccg ccatcacatt
     3241 ttaatcctga gcgtgtgacg ttttatcgcg ataaaccgga cgcccgtgct ttggcctggt
     3301 atctccctga ttcctatctc tgcgttctgc tcgacgggca ccataaggcc actgctgccg
     3361 cactggaagg gcgaccgctc aaaacactgg tactcagcac ggcaacccgt tttaatgatg
     3421 agcaacaaac cctggtattt ccgggcggag aatgtctgca caaaacagag ctactgtgcc
     3481 acgttcccaa actcactgag tggaaaacat taccgtccgg cgcatgggag agtttcggcc
     3541 ctgataagca catcagccca tccgaaacct ggtcagaaga gttgcagcag agcgtcagcc
     3601 ggtacccttc actggatcaa gcctggcaaa ttgttgaggc cgggaatttg agcgaaactc
     3661 gtattaagag catgatccaa caaggtttgg gggaggatga aaaggctgat gtaattttgc
     3721 aggcgctgtt tttcacacac tcgccgcttt ttattgactt tgcccgattc gttatcagct
     3781 atccggcata cgtcagttat cgccccctca ccttccggtt gatggcgcaa aaccgaacgc
     3841 cgcaagcaga tgcttttttt ctggattttg ccatcaatga tgacggcgaa cgtccggagt
     3901 tgacgaaaat aatggacgac tattttcgca agcgttaatc accccgcccg atgatgacag
     3961 cagccggaga gattttcgat aatcgggcag tcggcgctgt catcaccggg gcaggcattc
     4021 gccagcgcca gtagctggtt gcgcatggat tgcagttcct caatgtgccg ttcgatctcc
     4081 gccactttct ccagcgtgcg ccgtttcacg tcggcgctgt ggcgctgcgg gtcgttaaac
     4141 agattcacca gctcgccgct ctcttccagg ttaaagccca cctggcgcgc ctggcgcagt
     4201 aaggtcaatt cgttgagatg ctgctgcgtg taggttcgat aaccattttc gctgcgcatc
     4261 ggcggcgtca ccagcccctt ctcttcatag aagcgaatgg ctttgctggt caggccggta
     4321 atttttgcta catcgctaat gttcatcgtt cgcgcaacgc ctcctgtgct ttgttaaagg
     4381 gtttcaacag ataatccagc acgctttttt tgccggtgcg gatatcgacc gtcgccacca
     4441 taccggggaa tatcggaaac tgctgtttgc ttttgttttc cagatggttg ctgaaagtgc
     4501 gaatgtatac ccgatagtaa taaacatcgc gccgcacttc atcctgcacg gtgtctggtg
     4561 atatcaccgc cacttctccc ggcaggccgc cgtagatgga gtaatcgtag gcagttattt
     4621 tcaccagcga tttttgcccc gggtggataa acgccacatc gcgcggggag atttttgcct
     4681 caatcagcag ttgctcatcc agcggcacga tggtcatcag tttaccaccg ggggcgatga
     4741 cgccgccaac cgtggtgaca tcaatatcct gaacaatgcc tcgcaccggc gcggtaaagt
     4801 tgagcctggt gagagagtct tcccgtccac ggatcaccga acgctgggtt tccacttccg
     4861 cattggcttt tgctaactct tcgcgcgcct gaacgtagta ttgcgtgcgc acgtcgctga
     4921 gtttgttttc cagttcattg gcctggcgtt gcaggcgcag cacttcgacg ctgctcgccg
     4981 ccccctgttt caccagcggc tgggtcatcg ccagttcacg gttgaccagg ctaatcccct
     5041 gcgtcagccc ggtggtggct ttgttcaacc cgtcgcggcg agtgttgtac aacgccgttt
     5101 ccgactcgat aagttcgggg aatttattca attcgtcagg gaagaccaac ggtttattgc
     5161 tcacttccgc ccgcagccgc gccgaggttg ccagcgcagc gtacagccgc gacatcgctt
     5221 cctggacgtc ggattccgtt ttagtgcggt taagctgcgc cagcggctgg ccctgttcga
     5281 caatatcgcc aactttcacg ttcagatgat acagaattcc gccttccagc gactgcacca
     5341 cctgttcgcg cccggagggt tctaccgtgc cgctgccggt ggtgacttca tcaagctgga
     5401 agtaccacgc ccagccgaga aaacagagca gcattacagc ggtaacgcgt accgccagcg
     5461 ccgaacgcgg caagcggggt tcagtcaggc cacggttata ttgcagttgt ccgttcatgc
     5521 ggcagacccc ttattctttt cctgcaacac cacccgtcgc gccgtgggtt cgccctgttc
     5581 acgcaaaatg gcctctttcg cgccgtccat tacaatgcgc ccctgatcca tcacgataat
     5641 gcggtccacc agttgcagca tcgccgtgcg gtgagtggcg ataatcagcg tgcgatgccc
     5701 aatccactgt ttcagttgtt cgattaaatg cgcttctgcc atttcgtcca gcgaggcgct
     5761 gggttcgtcg agcagcacga tattgggttg agagatgagt agccgcgcca gcagcagcgt
     5821 ttgccgctga ccaccagaga ggccaaatcc cccttcctga atcatgtagt tgaggccgtt
     5881 tttctgcttc tgcacaaagg gcagcgctcc gctgagggtt aacgcgcgtt cgatatcttc
     5941 atcactggcc tccggcattc ccatggtgag gttttcccgc agcgaaccaa agaacagtcg
     6001 cccggtctgg ctgagtagcc ccatgtctcg ccgcagatct gccggatcaa gctgcccgat
     6061 actgatgtta tccagcaaca cctgcccctg ctgggcgata cgcattccgg cgagcatttg
     6121 cagtagcgtc gatttacccg cgccgttgcg cccgagaatg gcgattttct ctccggcctg
     6181 aatgtggagt ttgccgatcg ccacatcggc aatcttctct tcttcgtcgt aataaaagat
     6241 agcgttgctg aactgataat taccgtgcag caccgcttta tgcaccagtt tgccgtgttc
     6301 cggctgatcc actgggcgct tcatcagttc atccaggccg ttacgcgcca cctttgcctg
     6361 ctgccagcgc gagagcacgc cggatatctg cgacagcggc gcgatggtgc gtgaagccag
     6421 gatcgtcgtc ccgaccagcg cgccggtggt catgtcgccg ttcatcacca taaagcagcc
     6481 caccagcagc accaccacgt agacgatgga ctgcacttcc tgggtccagg tgagcagcag
     6541 cccggtgaga aaacgttgct tcatgctgat ggacgaggcc acgtcgttgg tgtgattcca
     6601 ctgattctga aaacgctgtt cggcgcgcag cagtttaata tcttcaatcg actgcacggc
     6661 ttccaccagc gtagcgttgc gtactgctga ctcgcgcatt ccttcgttcg ccagccgcgc
     6721 cagcgggcgt tgcaccagca ggccggggat caccagcaac ggcagcgcca gtaaaaccac
     6781 cagcaccagc cagccgccga tcatccacaa aataaagaca aacagcagga agaatggcag
     6841 gtccgccacc gcgccgatgg tggtggaagt aatcagctcc cgcactgatt ccagttcgcg
     6901 gatctgcgag ataaacgatc cggtggattt cgatcgcacg ttgtttttca gccgtaatgc
     6961 gtgaccaaaa acgcgatccg aaatacgcag atccgcccgc ttgccgatca catcagaaag
     7021 atgcgtacgc accatgcgca tacagaactc aaacaggatc gccatcatca ccccggcgaa
     7081 aagcacccat agcgtcggat aggactgcgc cggaaccacc cgatcgtaaa cctgcatcga
     7141 gaaaatcatc gctgccagcg ccagcacgtt cgccaccagt gacgccagca taatatcgcc
     7201 gtatcgccgc cagtctttca gcgagagatt ccagaaccag ttcgcctgat acgggcgaat
     7261 atagtcatcc acccgcgcgt cagggatggc gctgagtggg cgcagtagcg ccagttcaac
     7321 gattttatcc tcgacatccg cgacgttaag cgtggtttcc agcccttcgt caccgctgaa
     7381 ctgaacgctg atattgtcat gattgtcgcg acgggtgatc acgccaatct gctgattatc
     7441 aaacaccgcg atcagcggca gacgccaggg gttaagcgaa tcggcggaaa actccaccaa
     7501 ccgtaacccc attcccaact gccgcgccat gtcggtgagc aactcttccc gcttgctgcc
     7561 gcgctcccag ttgacggttg cgcgcacatt ctcctgagaa aaatcaagcc gataatagcg
     7621 cgccacggcg atcatcgcct ccagccaggc ttccacggac tgggcatttt ttttcattat
     7681 ctacaccacc atcaactgat gattcgccag caaagtggcg aggtcggtgt gcacgcccgt
     7741 cagcgtgacc acgttagttg tcgcaaaagt gccgcccgtg ccgtcgcggt cgatctgcac
     7801 gatggtgtcg ctgccgctct gggtgacttt gacaaagtca ccgatgtttc cggcctgcgc
     7861 atccagcgtc gccacgccgt tgacatagct ggctttgccg tttccggtgt agccgctgcc
     7921 ctgcaacaac tcgcggatat caatgcgatc ggtgtcggca gtgccttccc aggtgcctac
     7981 cgtaaagcca ttcaccacat ccgaaccgtt gccgccagtg gcgtcgctgg cgttgagcag
     8041 tttatagagc aaggtgtcgt ggccgccgtt gccgatattg agcgtgtcgt tgccgccgcg
     8101 cccttccagt tggttatcgc cgctgctgcc ggtcagagtg tcgttaaaat tcgatccgga
     8161 aatgccttcg atattggtaa atgtcgaggt attaaagcca gtagcctggg cggcggtgct
     8221 gcttaagttc gcggtcacgc ccacagtcga aagccggaaa tcaaccacgt ccataccgcc
     8281 gttcgccagc cacgttccgg tgccggagct gtacacccag ccgccggaac cgtcgtaggt
     8341 atcggtgcct gcggtggcaa agaaggtgtc gttataaccg gtgccgacga acttgccgtt
     8401 gttgctcgcg gtacccgctt cggcgctgag atcgtaagcg tgagtggcat cggtggcctg
     8461 taaaccgccc cagctggtgt tgaccgtctg gtcgatattg ctggcggtgg tgccggtgga
     8521 tttgatcagc accgcgttgt aggtttctcc ggcattcagc ccgtagaagt tgaccagcgc
     8581 cgaggtgtcg ttaaccccca tccccgactg cgggttaatg atttgcgtgg cgaccagcac
     8641 acccgccgag ttgtacagct tcacggtatt gccgtaatag acgttaatac cgttaggatc
     8701 ggtaatgcgc aggtgcagcg aagtaccgta gctcaccgtg ttggtgttgc ggatcaggaa
     8761 cacgctgccc gactgtttgg tcaccagtac atcaaccgcg ccgtcccagt cgtaatccat
     8821 tgccgccacg tcgctggtgg taccgctctg gctgccgccg agggccgatt gcgtccagtt
     8881 gctggcacca ccaacgttag tgtaaagata cgattgtccg gtctgggcga tacgggcgat
     8941 atccatcagg ccgtcatggt tccagtccac cgccaggcta aactggctgg cgtaggtggt
     9001 tgccgttgcg cccaccgcca ccgggctacc cagtacgccg ttggtgttga ataacagcga
     9061 gccgtagtta gctcttccct ggctggcggg caggaagaga tccatatcgc cgtcaccatc
     9121 gaaatcggcc caggtcatgg aaacgctgct gctcatagcg ccactgcccg tcccgctcag
     9181 gaaggtattg gtggtgttct gcccccagac aaacgtccca ttcccctggt tgatgagcga
     9241 agatagcgtg taatagttgt tcaggttgta ggtgtgctga atcaggtcga tcctgccgtc
     9301 attgttcaga tcgacgccaa aaccttcgtt gagcgaaaga taccccgtca ccgccccgcc
     9361 caccgtggcg ctaccgccgc tgttcgacgt ggtggagtta cctaccagcg tacctgcgtt
     9421 gttccacagg aaggtgttgg agtccggccc gcccgcgtcg ccaatccaga agtcgaggta
     9481 gccgtcgccc tctttatcaa atgccacaat cgagccgtac cacgtcaggg tgccgacggt
     9541 gacctgaata gcgctggaaa aggtgccgtc gccgttattg gtaaagccgt tgatatagcc
     9601 tgtaccgtag tcatcacggg tgataaacag atcgcccgtg ccgttacggt cgtaatcggc
     9661 aatggagctg gtggcgtagt tgttgccgct ctgatacagc gtcagcgcgg tcttcgacca
     9721 ggtgagtggg tcagtcgatt gcattacctg ctggtttgcc agcaccgtcc acatcccgtg
     9781 cgagtcgagg ccgtaggtca gcccccaggc ggtggttttg ctggcggtgg tccaggtcgg
     9841 tgtgtaatca atcgccgcgt taaccgtcac cgtgccgttg ctaatattgg cgttattgcc
     9901 gttacccgcc gaacttttta cctgcgcagt aacggtatag gcggtcgcgg aaactgtcag
     9961 cgcatcggta tccggcaact gtacatacca ggtgttgtgc gccggatcga ccactaccgc
    10021 gccgcccggt tcagaggtgt aggttttgcc gttgatcacc acttcaacat actgcccact
    10081 ggcaagcgcg gcggtgatca ccccgctgat aatcggcgta gtatcgcgcg tggtctggct
    10141 ggtgatttgc gccagcgtgg tcggaataga ggtatcgacc gtcaggacaa aatcactgga
    10201 ggaggtgata ttccccgcca aatccaccac tcgcgcgctg taggtatagg caccgctcac
    10261 cagcccgctg tcgctgtagg tccagttcag agcgccgacc atcgtcaccg cacctaacag
    10321 cagcccgtta cggtagaggt aaaccacttc accgctggca agcggcgcgg agagtacacc
    10381 gttcagcagc ggcgtagtgt cgtcggtggc ctgcgaactg cttaatgtcc cctgccgctg
    10441 cccgacatca tcggtatacg acacaatggt tggcgtggcg gtagggttaa tcgtatcgac
    10501 caccaccgtt ttcgatacca ccgggccggt gttccccgcc agatccagca cccgcacctg
    10561 ataaacgtag ctgccgtcgg tcagcgtgcg gctgtcggtg taagtccact gtgtaccgtt
    10621 ggtggtgagc gtgatccagg tcgcgccgct atcaaggcta atctgtgcca cttcgccgct
    10681 ggcaagcccc gcccccagcg aaccggtcag cgtgagcgtg gtatcactgg tgataaagtc
    10741 ggttgccgaa ctccccgtgt cggtgctaat gctggcgatg gtgagcacct gcgccgggtt
    10801 aacggtgtca atctgcgccg actggctgcc ggttgcgcca acgttgcccg ccagatcgac
    10861 cacccgcacc tgccaggtgg tggtgccgtt ggtgagggtt cgtccgtcaa cgtaactcca
    10921 gttcagaccg tctgctgcta gcgtcacgtt cacccaggtt gcgccgttgt cggtactgat
    10981 ttgtgcgaac tcattagcgc cgagcgccgc gcccaacacg ccgcgcacgg taagcgtggt
    11041 gtcgctggta ataaaatcgc tgctgctggc tccggtgtca tcgctgatac cggtaatggt
    11101 gatacttttc gccgcttccg ggctggtggt gtcgaccacc acgttttgcg ttgcggtctg
    11161 cccaacgttc cccgccagat ccaccacccg caccgtgtag ttccaggtgc cgtcagtaag
    11221 cgtgcgacca tcggcatagc tccagctggt gccaacgacg gttaacgtag tccaggtgac
    11281 accgccgtcg agactgattt gcgcccgttc atcggcggca agcgccgcgc cgagcgtgcc
    11341 gcgcacggtc agcgtggtgt cgcgagtaac aaaatcgctg ctgcttaacc cggtgtcatc
    11401 gctgatgccc gcaatggcga tggttttgct cgccagcggc gcggtggtat ccacggtgac
    11461 tatctggctt gcggtggcgc cgatatttcc ggcggtgtcg atcacccgta cctgatagtt
    11521 gtagttgcca tccgtcagcg tacgcccgtc aagccaggtc caggtcaaac cgcttaccga
    11581 aagattctgc caggtggtgc cgccgtcgat actgatttgc gcgaactcac cggcagaaag
    11641 cgccgcgccc agcgtgccgc tcaccgcaag cgtcgtgtcg ctggtgacaa aatcgttagt
    11701 gatcaatccg gtatcggtgg tgatcgcgct gatggcgatg gttttcaccg cgggatctgg
    11761 cgcggtagtg tcgatcacca cattctggct gtcggtcgcg ccaacgttgc ccgctgcgtc
    11821 aatcacccgc acctgataga gatagttgcc atcggtcagc gtgcggctgt cgttatagcg
    11881 ccaggtcgtg ccggtaacgg taagcgtggt ccaggttgtg ccgccgtcga tacttatttg
    11941 cgccgtctcg ttactggcaa gcgcggcggt gagcgagccg ttaaccacca ccggactgac
    12001 gctggtgata aagtcgctgg cgctaaggcc agtgtcggct tgcagggaat cgatattaat
    12061 acccatcgcc ggggcgctga gatcgacggt aatggtgtga ttgctgctgc cggtattgcc
    12121 aatggcattg ctcaccgagg cgttaatcac gtaggcgttg ccgtccgcca gcgcggtgac
    12181 atcggcgctg ccgagggtgt agctccagct accgcccgtc tgcaccgtgg tggtgtaggt
    12241 tttgccgttt aaggtcaggg tcagcgtctg cccgacgggc gcggtggtgg tgccgctgat
    12301 taccagcgat gagccgtgtt ccgctgcatt caccacatcg tcgcccgcaa aggtgttgat
    12361 ggtgacggtg ggcacgtcgc cgttcagcgt cacgccacgg ctggcgctgc cggggtttcc
    12421 agcctgatcg ctgaccgtcg cgctaatggt gtagctgccg tcgcttaatc cggcaaactg
    12481 ctgcgccgga ataaataccg accagcttcc gccgctgccg accgtggcgg tccagctctg
    12541 accgttaaag gtgacggtca ccgtctggcc cacttcggca gtggtggtgc cgctgatggt
    12601 ctgcccggca agttgctcgg cattattgat aatatcgtca cccgccaccg tggcgatggt
    12661 gaccgtcggc gcaacggtgt cgacggtcag ggcgtgcgtg gtttgtccgg ggttaccggc
    12721 gcgatcgttc acgctggcgg taatggtctg cgcgccctgc ccgagcgcgg ccagatccgc
    12781 cgcaggcacg ttcagtgccc aggtgccatt ggcggcaacc gtggcggtat aggttttgcc
    12841 gccaaaggtg acggtgaccg tctgccccac ttccgcgctg gtcgagccgt taagggttaa
    12901 cggctgctgc tgttcggcgg cgttgagtcg gtcgtccgtc gaaacggtgt tgatgctgat
    12961 aaccggcgcg gtgagatcca ccgtcacgct gcgcgaggcc gtggcgctgt ttccggcgct
    13021 gtcctgagcg gaggcgctga ccgtgtagct ggtgccatcc gccagtgccg caacgtctgc
    13081 cgcaggcacg ttcacgctcc agctcccgtt gctctgaatg gtggcactgt aacctttgcc
    13141 gttaagcaac acggtcacca ccgtacctgc ggcaaagttc gttccggttc cgctcagggt
    13201 cagcgcactg cccgcttcgg cggcgttaat aaggttatcg ccgctgatgg tgctaaccga
    13261 gagcgacact gccgccgtgt tcacctgcac gttgtgcgtc tgcgtgctgc tgttgcctgc
    13321 gctatcggta atggtggcgc tgatggtcac cgtgccatcc gcaaggccag aaatcacgct
    13381 ggcaggcacg ccgacgctcc agttgccgct ggcatcggtg ctggtgacat actgctgccc
    13441 ggcgatggtc accaccaggc ggtcgcccgc caccgcgccc gtggcggtgc cgctgataat
    13501 ctgcgcctga gtgtgttcga cgttgttaat cacatcatcg cccgccacgg tgttaaaact
    13561 gatgacgggc gcggtggtat cgacggtcac acctttgctg gcgctgccag ggttacccgc
    13621 cagatcgctg accgtggcgg tcagggtata gccgttatta gcaagcgcgg ttaagtcgct
    13681 ggcaggcagc gttaagctcc agctgccgtc tgcctgcacc gtggtctggt agttagtgcc
    13741 gtttagcgtg acgatgagcg tttgccccgt ttgcgccgtg ctggtgccgg agaccgtcac
    13801 acctgccgct gcttcgctgg cgttgatgat gttatcgctg gcaagggtgt tgatggtgac
    13861 cgttggcgcg gcgctatcga cgctgtattc gtgcgttgcc gtggcgctgt tgccgttgac
    13921 gttagtcacc cgcacctggg cgctggcatc gccgtcacgc agcgccgcca aatccgcagc
    13981 cggaacggtg gtgctccagc taccgttagc cgccaccgtg gtggtgtagt ttttgccgcc
    14041 aaaggtgacg gtgacggttt gccccgcttc gacgccagac gtgctgccgg agagcgttag
    14101 cgccgcgcct ttttcggcga cgttaatcac atcatcgctg gtgatgctgt tcatgctgac
    14161 ggcgacggtc gtgagatcaa cagtcagcgg atgggtaatg ctggtttgcg ttccggcact
    14221 gttcgccccg ctaacggtaa tattcagcgt tcccgcaggc cagttactga catccgtcgc
    14281 cggtacgccg acgctccagg taccattcgc caacaccgtg gcggcgtaag tttggttatt
    14341 aatcaccacg gtcagcgccg tgcctgcggc aaagccggag ctgctgccgg tgatcacctg
    14401 cgcctgagcg tgttcgatga tattgaccac atcatcaccc gcgacggtat ccacccgcag
    14461 gccaggcaga ctggcatcga tggtgatatc gcgcgtgccg ctaccggtgt tgccaacggc
    14521 gttggtcacc gaggcattaa ccgttaactc accgttaccc agagcctgcc agtcggcggc
    14581 aggtacgctc acattccagc ttaaatcagc ctggacggtg gcggtgtaag ttttaccgcc
    14641 cagttcaacg gtgacggtgc tgcccgccgc cgccccggtg acctggccgc tgatggtttg
    14701 cgcactgcct gcttcagcgg cgttgataat atcgtccgtc gccaccggat taatggtgat
    14761 gcccggcagc gcggtattaa cctgcacgtt atggctggcg gtgctgctgt taccctgagc
    14821 attggtgacg ctcgccgtca cgctgtaggt cgcttcgccc agcgcactga ccgccgaggc
    14881 aggcacggta acgctccagt tgccggaggc gtcggtagtg gcgctgtagt tttgtccgtt
    14941 cagcgtgacg atgatcgccg tgcccgctgg ctgatcgctg gtgccagaaa gcgccagatc
    15001 agatcctttt tccgtcgcgt tgatcacatc atcgcccgcg atggtgttga tgctaatcac
    15061 tggcgcgctg aggctgacgg tcacagtgcg gctggcatcg tcgctgttgc ccgcccgatc
    15121 gctgacgctg gcggtgatgg tctgcgcgcc gctaccgagt gccgtaacat ccgccgccgg
    15181 tacgccaacg ctccagttac cggaggcatc cagcgtggtg gtgtaggttt tgccgttgat
    15241 cacaacgctc accacatccc ccgcttcccc gcccgtgctg gtgccggaga tcaccagcgc
    15301 ctgggcgtgt tcggcgtcgt tgatgatgtc atcgcccgcc acggtattta tggtaagaac
    15361 cggcgcggtg gtgtctaccg ccagattatg gctggcgctg ccggggttgc ccgctttgtc
    15421 gttgaccgtg gcgctgacgg tgtaattgct tgcggtcagc gcgcctaagg ctgaagtggg
    15481 aacgctgact ctccagctac cgtctgcctg cacattaccg ctgtaggttg cgccgttaag
    15541 ggtgacggtc accgtctgcc cggtttcggc ggtgctggtg ccgctgatgg tgagcggatt
    15601 tcccgcctct gtggcgttaa ggatatcgtc gctggcgatg gtgttaatgg tgagcgtcgg
    15661 cgcgctggcg tcaacgctat acgcatgggt cgccgaggca ctgttaccgc tcgcgctgct
    15721 gacgctggcc tgcacgttcg ccgcgccgtc tggcagggtt gccagatctg ccgccggaac
    15781 cgttatgctc cagcttccgt tggcagcgac gcttgcggtg taggttttgc cgccaaaagt
    15841 gacggtgacg gtttgcccgc tttcaatccc gctggtgctg ccggaaaggg tcaggtcagt
    15901 gcctttttca gcggcgttaa tcacgtcgtc gctggcaacg gtgttgatgc tgatcgccac
    15961 cgccgttaaa tcgacggtga acggatggct gacgctgacc ggatttccgg cgctgctctg
    16021 gcctgtcaca tcaacggcta acggccccgc aggccaggcg ctgacgtttg ccgccggaat
    16081 gccgacgctc cagctaccgt ccgcctgtac cgtggcggaa taagtcgtac cgttaatcgt
    16141 gatcgtcaac ggcacaccag cattcaggcc gctactgcca ccggtgatta ccagtgcctg
    16201 cccgtgttca atgctattga cgatatcatc gcccgccacg gtatccaccc gcagccccgg
    16261 caggttggcg tcaatggtga tatcccgcgt gccgctgccg gtgttgccat taccgttggt
    16321 gactgaggcg ataatggtca aatcaccatt gcccagcgcc tgtaaatcgg ctgctggaac
    16381 gctgacgctc cagcttaagt tgccctgtac cgtagtggtg taagtgttgc cgcccagcgt
    16441 cacggtgacc gtatcgccag cggcagcgcg agtcaccacc ccgctgatgg tttgattggc
    16501 acccgcttcg gcggcgttga taatatcgtc gcccgccacg gtgttaatgg tgacagccgg
    16561 caggccgcta tcgaccagta aattagcctg gctactggcg gtgttgcctg ccgcactggt
    16621 ggcgctggcg ctgaccgtat agttggcctg gcctaaagcc gccagatcgc tcaccggcac
    16681 ggttaaggtc cagttgcccg acgcgtcggt ggtggcgctg tagttatgac cgttgagcgt
    16741 gacggtgacc tgcgcgcccg tcgccagccc cgtactgccg ccgctgatgg tcaacggctg
    16801 gcctttttca tcggcgttca ggacgttatc gccgctgatg gtgttaaagg taatggttgg
    16861 caggccagta ttgaccgtca cctgatgggt ggtgctgccg ctgtttcctc cggcatcggt
    16921 gacgctggcg ttaatggtca ccgtgccgtt cgccagtgcc gagacgacgc ttgccggaac
    16981 gccgacactc cagttaccgc tggcatccag cacggtggta aaggtatttg tgccgatagt
    17041 caccgtcacg gtgctaccgg ttgccgcacc ggtggcggag ccgctgatga tctgcgcctg
    17101 ggcgtgttcc gtcgcgttga tcacatcatc gcctgccacc gtgttaatgg tgacgaccgg
    17161 aacagaagta tccaccgtca gattatggtt aaccgaggcc gggtttccgg ctttgtcgtt
    17221 caccgccgcg ctcacggtgt agttgctggc ggtcagggcg cttacgtcgg cttgcggcac
    17281 actgacgctc cagctaccgt ccgtttgaac cgtgccagta tagttttcgc cattgagggt
    17341 gacggtcacc gtctgccccg cttccgccgt gctgctgcca gtgatggtca gcgccgcgcc
    17401 ggcttcggcg gcgttaagaa tgtcgtcgcc cgcaatggtg ttaatggtca ccgttggcgc
    17461 gctggcatcg acgctgtaag cgtgagtagt ggtggcgctg ttgccgttaa cattgctgac
    17521 gctggcctgc gcgctggcat cgccatcacg cagggccgcc atatctgccg ccggaaccga
    17581 ggtgctccag gaaccattcg ccgccaccgt ggcgctgtaa gttctgccgc caaaggtgat
    17641 ggtgaccgtt tgtccggcct ccacaccaga agtgctgccg gagagcgtta acgccgcgcc
    17701 tttttcggca gcgttaatca cgtcatcggc agtaatggcg ttgatgctga ccgccaccgc
    17761 agtgagatcg acagtcaccg gatgcgtaac gctcaccggg tttccggctg tagtgttgcc
    17821 gctcgccgta attgtgacct tccccgcagg ccaggcgctg acatctgccg ccggaacgcc
    17881 gacgctccat gagccatccg ccagcaccgc cgcaacatac gtttgcccgt ttatggtcag
    17941 cgtgacgttg ctgcccgctg ccagatcgga gctgctgccg gtaatcacca gcgcctgatt
    18001 gtgctcgata atgttgacca cgtcatcgcc cgccacggta tcgacccgca gacctggcag
    18061 attcgcgtcg atggtgattt cacgcgtgcc gttgccggta ttgcctacgc tgttcgttac
    18121 cgaagccgaa atggtcagct caccgttgcc caacgcctgt agcgcggagg ccggaacatc
    18181 gacgctccag cttaaattcg cctgcaccgt ggcagtgtaa gtcgccccgc cgagcgtgac
    18241 ggtcaccgtg tcgcctgccg ccgcacccgt cacctgcccg ctgatggtct ggtccacgcc
    18301 cgcttcggcg gcgttaataa tatcgtccgt tgccaccacg ttaatggtga cgcccggcag
    18361 cgtggtatta acctgtacgt tatggctggc ggaaccgctg ttgccatcgg cgtcagtggc
    18421 ggaggcggtc acggtataag tcgcttcacc aagcgtacca accgcagacg ccggaacggt
    18481 cacgctccag ttcccggaag catcggctgt ggtggtgtaa tgttgcccgt taagcgtaac
    18541 ggtaatctgc gtgcccgcag gctggtcgct ggtgccggaa atcgccagat ccgcgccttt
    18601 ctccgtggcg ttgatgatgt catcgacggc aatggtgtta atggcgagca ccggagcgcc
    18661 gagcgccacg ctgacaacgt gcgaggctgt gccgctgttg ccggcggagt cggtaaccgt
    18721 ggcgctaatg ttcacatcgc cctgcgccag cgcggaaatg acgctggcag gcacgccgat
    18781 gctccagttg ccgttggcgt ccagcacggt ggtgtaggtg gtcgtaccaa tcgtcacgga
    18841 aaccgtgtta cccgtcgtcg cgcccgttgc cgagccgctg atgatctgcg cctgtccgtg
    18901 ttccgtggcg ttaatgatgt catcgcccgc taccgtgttg atggtgacga ccggagcggc
    18961 aaggtccacc gtcagattat gcgttgccga agccggattt ccggctttgt cgctgaccga
    19021 ggcgttaacg gtatacgagc tggcggtaag actagctaaa tcgccggttg gtacgctgac
    19081 gctccagctc ccgtctgcct gcacattgcc gctgtaatta acgccgttta gcgttacggt
    19141 caccgtctgc ccggcttccg ccgtgctggt gccgctgatg gtcagcgccg atcccgcttc
    19201 ggcggcgtta agaatatcgt ccgtggcgat ggtgttgatg gtaacggagg gcgcagtggc
    19261 atcaacgctg taagcatgcg ttgcctgggc gctgttccct gcgacgttgc tgacgctggc
    19321 ctggacgttt gctgcgccgt ctggcaaggt tgcaacatcg gcggcgggga tcgtcagccc
    19381 ccaggtatta tccgcggcaa ccgtggtggt gtagcttttg ccgccgaaga taacggtgat
    19441 ggtttgcccc gcttccacat cggaggtggt gccggagagc tgcaaatcag agcctttctc
    19501 agcggcgtta atcacatcgt cagtggaaag ggggttgatg gtgatcgcca cggctgcgag
    19561 atcgacggtc accggatggg taatactggt tgtcgttccg gcggtgttgg tgccagagac
    19621 ggcaatattg accgtcccct caggccagtt tgtgacatct gccgccggaa caccaacgct
    19681 ccagctacca tccgctaaaa ccgttgcgcc gtaggtgacg ctattgataa ccaccgtcaa
    19741 cgccgcgccc gccgccagtc cgctactgct accggtgatc accaacgcct gagtgtgctc
    19801 gatgctgtta atcacatcat cgcctgccac ggtatcgacg cgcagacctg gcaggttggc
    19861 atctatcacg atatcgtgcg tcgcggtgcc ggtattattt gcggaattgg tcaccgaggc
    19921 gctgatggtc agttcaccgt tacccagcgc ctgcaaaacg tttgcaggta cgctgacgct
    19981 ccagcttaaa tctggctgta cggtggcggt gtattggttg ccaccaatag tgaccgtcac
    20041 cgtgttaccc gcagccgccg ttcccgtgac ctgcccgctg atggtctgcg ccacagcgat
    20101 ttcagcggcg ttgataatgt catcgcccgc aacggtatta atgatcaccc caggcaggag
    20161 cgattcgaca ttcaccgtat gggtggtgct gccgctgttg cccgcgctgt cggtggcgct
    20221 ggcgctaatc tgatacaccg cctcacccag tgcgccgacc gccgaagcag gcacggtaac
    20281 gctccagttg ccggaactat cggtggtggc gctgtacgcc aggccgttca acatcacggt
    20341 gaccgtggtg ccagtcgcca gaccgctgga gccgccgctg atggtcagat cctgggtttt
    20401 ctccgcatta ttaataatgt catcgccgga gacgatactg atggtcaacg tcgggccgct
    20461 gagatcaacg gtcacattat gggtagtgct gtcgctgttg cccgcgcgat cgctcaggct
    20521 ggcggtgatc gtctggctac cggttgccag cgccgtgaca tccgccgccg gaatgcccac
    20581 gctccagtta ccggaggcgt ccagcgtggt ggtgtagttt ttaccgttca ggttaaccgt
    20641 gaccacatcg cccgccgccg cgcccgtact ggttccgctg acgaccagtg cctgaccgtg
    20701 ttcgatggcg ttgataatgt catcgcccgc aacggtgtta atggtgagat ccggcgcggt
    20761 aacgtccacc gccagcgcgt ggtcggcgct tgccgggtta cccgctttgt cgctcaccgt
    20821 ggcggtcacg gtgtaactgc tggcggttaa tccgcttaaa tccgctgccg gaacattcac
    20881 gctccaggtg ccatccgcct gaacggtggt ctggtacgtg ttgttattga gcgtgacggt
    20941 gagcgtctgc ccggcctgcg ccgtggtggt accgctgatg gttatccccg cgcccgcttc
    21001 gctcacgtta agagtatcgt cgctggcgat ggtgttgatg gtcacaagtg gcgcggtggc
    21061 atcaacgctg tacgcgcgat ccgcctgggc gttattgccg ttgatgttgc tgacgctcgc
    21121 ctgcacgttg cccgcgccat ccggcagcgc agcgagatcg gcaggcggca cattcaccgt
    21181 ccagctcccg ttagcttcca cagtggtggt gtaatttttg ccaccaaagg tgacagccac
    21241 cgtttgccca gcttccacac cgctggtgct gccgcttacg accagcgttt cgcctttttc
    21301 gacggcgttg atcacatcat cgccggaaag ggtgttaatg gtgatggcga ccgccgccag
    21361 atcgacagtg accggatggg taatggtgga ggttgttccg gcgctgttgg tgccgcttac
    21421 cgtaatatcc accgtacccg caggccagtt gctgacgtct gccgccggaa cgccaaggtt
    21481 ccatgtgccg tcggctaaca ccgtcgcggc gtaagtgacg ttgttaatct cgaccgttaa
    21541 cgccgttccc gctgttagac cggtgctact gccggtgatc accagcgcct gattgtgctc
    21601 aatgctgtta ataacgtcat cgcctgccac tgtatcgacc cgtaagccag gcagattggc
    21661 atcgatagtg atatcgcgcg agccgctgcc ggtgttgcca acgccattgg tgaccgaggc
    21721 gttgaccgtc agggaaccgt tgcccagcgc ctgaatatcc gccgctggta cgctaacgct
    21781 ccagcttaaa ttagcctgca ccgtggcggt gtaggtgttg ccgcccagcg tgatggtgac
    21841 cgtatctccc tgcgccgcgc ccgtcacctg cccgctgata gtctgtgcat tgcccgcttc
    21901 ggcagcgttg ataatgtcat cggtcgccac cgcgttaatg gtgacggcag gcaaggcgct
    21961 gttgaccaac acattatggc tggcggaatt gctgttgcct gccgtatcag tgacgctcgc
    22021 cgttaccgtg tagttagctt cacccagcgc gctaaccgct gacgctggca ccgtggcgct
    22081 ccagttgccg ttgctatcgg tagtagcggt gtaattttgc ccgttcaacg tcaccgtaat
    22141 ggtggtgccc gcgggctgat tactggtgcc ggtgatttgc aggtccgcgc ctttttccgt
    22201 ggcgttaatc acatcatcgc tggcgatagt gttgatgcca atggttggcg cggtgagatt
    22261 tatggtgacc gtgcggctgg catcatcgct gttgcctgcc gcgtcggtaa tggccgcagt
    22321 gatgatttgc ggaccgctgc ccagcgcagt aacgtctgcc gccggaacgc cgacgctcca
    22381 gttgccggag gcgtccagcg tggtggtgta ggttttactg tttaacgtga cggtgatgac
    22441 atcgcccgct tcgccgccag tactggaacc ggagatcacc agcgcctgcc cgtgttcagt
    22501 agcgttaata atgtcatcgc cggagacggt gttaatggtc agcaccggca cggtgagatc
    22561 caccgccagc ccgtgagtgg cggacgccgg gttacccgct ttatcgctta ccgaggcgct
    22621 gacggtatac tgactggcgg tcagattgct gagatccgcc gtcggtacgc tgacgctcca
    22681 gctcccgtcc gcctgaacgg tgccactgta agtcacgcca ttaagtgtga cggttaccgt
    22741 ctgccccgct tcggcggtac tgctgccgct gatggttagc gggttgcccg cctcggcggc
    22801 gttcagaata tcgtcagtgg cgatggtatt gatggcaagc gtcggtgccg tggcgtcgac
    22861 gctgtaggcg tgcgttgccg aagccgtgtt gccgttaata gtgctgacgc tggcctgtac
    22921 cgtggcgtcg ccgtcgcgta acgcgctgag atcggcggca ggcacggtgg ttgtccagct
    22981 accatcgccc gctacggtag cggtgtaggt tttcccgcca aaggtaacgg tgaccgtttg
    23041 ccccacttcc acgccggagg tgctgccgga aagggttaaa tctgcacttt tttcagcggc
    23101 attaatcaca tcgtcgccgg aaacggtgtt aatggaaatt gccacagctg ccagatcgac
    23161 ggtgactgga tgggtgatag tcgtagtcgt ttctgcagtg ttagtgccgc ttacaatgat
    23221 attcactgta cccgcaggcc agttgcccac gtctgctgcc ggcacgccaa cgctccatgt
    23281 gccatcggct aatactgttg cagcgtaagt gaccgtgtta atgactaccg tcagcgccgc
    23341 gcccgccgcc agcccgctgc tgctaccggt tatcaccagc gcctgagcgt gctcgatgct
    23401 attgaccaca tcatcgcccg ccacggtatc gacgcgcagg ccaggcaaat tggcgtcgat
    23461 agtaatatct cgcgaaccgc tgccggtatt gccaacgccg ttggtgaccg aggcgttaac
    23521 cgtcaggtcg ccattaccga tggcctgaat atccgcagcc ggaacgtcca cgctccagct
    23581 taaattcccc tgcacggtag cggtgtaggt tttcccgcca agcgttacgg taaccgtatc
    23641 gcccgccgcc gcgcccgtga cctggccgct gatggtctgc gccacgcccg attcggcagc
    23701 gttgataata tcgtcgctcg ccaccggatt aatggtgacg ccaggcagcg cggtattgac
    23761 ctgcacatta tggctggcgg aattgctgtt gcctgcgctg tcggtgacgt tcgccgtcac
    23821 cgtgtagctg gcttcgccca gcgcaccgac ggcagaggct ggcaccgtgg tgctccagtt
    23881 gccagaagca tcggtcgtcg cggtgtaatt ttgcccgttc agcgtcaccg taattgtagt
    23941 gcctgcgggc tgattactgg tgccggaaat ttgcagatcc gcgccttttt cggtggcgtt
    24001 aatcacgtca tcggtggcga tggtgttaat gccaatggtc ggcgcggtaa ggttaacggt
    24061 gaccgtgtgc gtctcactat cgctgttacc ggcggcatcg gtaacggtcg cagtcacggt
    24121 ttgcgggccg ctacccagcg cggtgacatc cgccgccgga acaccgacgc tccagttgcc
    24181 ggaggcatcc agcgtcgtgg tgtaggtttt actgtttagc gtgacgctga cgacatctcc
    24241 cgcttctccg ccagtgctgg aaccggaaat caccagcgcc tgcccgtgtt cggtggcgtt
    24301 tataatgtca tcgcccgcaa tggtgttgat ggtcagcacc ggaacggtga gatccaccgc
    24361 cagattatgc gtggtgctcg ccgggttgcc cgctttgtca ttgaccgtcg ccatcagggt
    24421 gtggctgcca tcggtcaagg ctgcgagatc tgccgccgga atattgatgc tccagctgcc
    24481 gtcggcctgt accgttgcct gatacgtctg cacggtcggg ctattgagcg ttaccgtaac
    24541 aatctgcccc gcttcagcgg tggtgctgcc gctcaccgtc acacccgcat cggcttcgct
    24601 ggcgttgacg atattgtcgc tggcgacggt attgataatg atggttggcg cgctgctgtc
    24661 gacgctgtag ctgtgcaccg ccgaggcgct attgccgttg atattgctga cgctggcctg
    24721 cgcggaggcg ctgccctctg gtaatgctgc cagatcggcg gcgggaacgg tggcagtcca
    24781 gctaccatcg cccgccacgc tggcggtgta atttttaccg ccaaaggtga cagtgacggt
    24841 ttgccctgct tctacgttag tggtggtgcc ggaaagagtt aaattagccc ctttttctgc
    24901 agcgttaatc acgtcatccg tggcgatggt gttgatggtg atcgccgccg gagtgagatc
    24961 caccgttacc ggatgcgtaa tgcttatcgg atttccggcg ctactttccc cggatacggc
    25021 aatattaacc gccccgcagg ccaggcgcta acctgcgccg ccgtgatgcc cacgctccag
    25081 ctgccatcgg cctgcaccgc agtggtgtat tccacatcgt taatcgtgac ggtaagcggc
    25141 gtgccttcag ccagccccga gctgctgccg gtgaccacca gcgcctgccc gtgctcgatg
    25201 atattgacca catcatcgcc cgccaccgta tcgacccgca ggccgggcag attggcgtcg
    25261 atggtgatat cccgcgtgcc gctgccggtg ttgccgtttt gattggtgac cgaggcatta
    25321 accgttaaat cgccattacc cagcgcctga atagcgtccg ccggaacgtc cacgctccag
    25381 cttaagccgg cctgcacggt ggtggtgtag gtattaccgc ccagcgtcac ggttaccgcg
    25441 tcgccaacgg cggctccagt cacctgcccg ctgatggttt gcgccacgcc cgcttcggcg
    25501 gcgttaataa tatcgtcggt tgccaccgga ttaatggtca caccgggcag cgcgctgtcg
    25561 accagcacgt tatggctggc ggtggcgctg ttgccgatac tgctggtcac cgccaccgtt
    25621 accgtgtagt tggcctggcc cagtgcggtt accgccgatg ccggaacggt gacgctccag
    25681 ttgccggagg cgtcggtcgt cgccgtataa ttttgcccgt tcagcgtcac ggtgatggcg
    25741 gtgttaacag gctgatcgct ggtgccggta atctggacat cggccccttt ttcgctggcg
    25801 ttaatcacat catcgcccgc aatgctgtta atgccgataa ggggcgcggc ggtattgacc
    25861 gtgacggtca atgactggct gcccgtgttt ccggctttgt cggttaccga gacgctgaca
    25921 ggataactgc cgtccgccag cccactgacc accgaggccg gaacgcccag actccagttg
    25981 ccggaaccat ccaccaccgt ggtgtaatcc atattattca gcgtcacggt gacgatatcg
    26041 cccgcctgag cgccagtgac cgtaccgctg atgatttgcg cctgggtatg ttccggcgtg
    26101 ttgatgacgt tatcggtcgc caccggatta atggtcacca ccggcggcgt ggcatcgacc
    26161 agcgccacgc gcgaaacgct ggtcgggtta ccagcaacat cgttcacgct ggcggtgacc
    26221 gtggcgttac cgtcagccag cgcgtcaacg ttggcggcgg gaacggtcac gctccaggtg
    26281 ccgtctggct gcacgacgcc ctgatacgtc tgcccgttca gcgtaacggt caccgtctgc
    26341 cccggctgag cgtcggtggt gccggtgatg ggcagatcct gcccgctttc ggcggcgtta
    26401 atgatgttat cggcggtcag tggatcgatg cttaaggccg gggcctggct atcgacggta
    26461 atggtgcggg aagtgttgcc ggtattgccg ctgagatccg tcacagaagc ggtaatcgcc
    26521 accgcgccgt caggcagatt tcccatcgcg gcggcaggta ccgtcagact ccaggagcca
    26581 tcggcctgca cggtagtggt aaaactctgt ccagccaggg ttactgtgac ggtttgtcct
    26641 gtctcagcct gttgagtgct accggtaagg gtcagtggcg cacctttttc tgcgccactg
    26701 ataatgtcat cgccggagac ggtgttaatg gtgatcaacg gagaaacacc gaccagcgtg
    26761 attgctcgag acgccgcgcc agtatttcct gcagcatctg tcacggtgac gaaaatcgta
    26821 ttggctccgc gatccagcgt gcgggttacc gccgggtcca acgccacatt ccagctgcca
    26881 tctgccagca cgatgcccgt caggacatga gtgcccaatt tcaccgttac cgtatcgcca
    26941 ggagaagcac cgctaacttg tccggagatg atctgcgcga cggcctgctc ggcattatta
    27001 agaatatcgt cgcccgccac ggtattaaca ctgaccacgg gtgctgaggt gtctaccgtg
    27061 aaattcgcgc tgtggctggt ggtgttccct gcccgatcgc tgacactggc attgacagcg
    27121 taattaccct ccgccagtgc ctggacttcc gtggcaggca gcgtcacttg ccaggttccg
    27181 tcgctaccga cggttgctgt atggcttttc ccgttgacgg tcagtgttac ggtttgcccc
    27241 gcttctgcat tcgacgtgcc gctcagtacc agcgcgacgt tatgttccgc agcactgatg
    27301 atgttgtcct gagcgatggt gttgatggca agcgttggcg gttgggtatc gaccgttagc
    27361 agctgcgcgc cggtgaccgt attgcctgtg gtgtctttcc cactgaccag taccgcctga
    27421 ttcccttcgc ctaacgcctg tgcatcggcg gtcggcacct gcacgctcca ggcaccgtta
    27481 gcgcctacgc tggtggtgta ggttttgccg ttaagtgtga cggtgagttc tgttccctgt
    27541 gccagatggc tactggtgcc agaaagcgta aacccggcgg cctgttcgct ggcgttaata
    27601 atgttatcgc ctgcggtggt ggcaacctgc acgctggcct gactattgtc gatggttaac
    27661 gttatcgaac cggcgtcagt gatggtactg ccgacaattt gcaccactga ccaggtatgt
    27721 tcacctgcac tttgcgtggg taacgagaca ctccagtgac cgttgttgtc tacctgagta
    27781 ctggcaatag tattgccgtt actgtcttta atctgaatag tcgcccccgc ctgcccgctg
    27841 ccgctaaagg ttggcgtggt gtcatcggtg atgtcattgg tggcgagaat gccgcgctga
    27901 tcgccctgat tatcggtaac taaaaatgat ggtgtggcgt tgccaggctc agcgggtggc
    27961 gtagggttat taatcacttc tgttttcgag tcgccatcgc caccgcttgc cagcagtgca
    28021 ccaagcgcac caccacctac cgccgccccc gccagccatc cccacgggaa ggcgctggtc
    28081 tgagcaacgg tatcaagaaa cggcgcaatg ctttcaatcg cagtggtctg ggcagtcagt
    28141 tctacggggg ctaatccacc cgcagcagta tcggcaaagg tgacatgggt tagctgttgc
    28201 ccatcggcaa acaccagttc cgattgttca gccgtattcg ccgcttgcag gaaataaccg
    28261 ttgcagcgga tcaccgtgcc gtcctgcata tagatcagca ggtcatttcc ttcacggacg
    28321 taacgcgcaa cggcagaggc ctgagcatga acctgcacca cggaaggctc ataaataaca
    28381 accgtgacat cgccaggatt aatcagcgtt tgtttgacgg atgttttacg cgaaataaca
    28441 tcaataatca ggctcataat ggcattcctg ataaatggcg cacatcctta gcgagtccat
    28501 ttatctaaat aagacaaagg taatcactta acaacgaggc atacctgata ttaataatgg
    28561 caggattatt tcaagtcggg taagtcgttg tataagccct gtttaattcc cgctaatgga
    28621 ataaggttat tcaccgcagc ggcataattt acggcggcga cccagccatc ataattggca
    28681 tttatttcag ccgattgcgc ctgaaagaca tcttgctcga cggttaatag atcattcaga
    28741 ctgcgtttac ccaatttata ttcattttgg tacacatctc gcgttttgtg cgcactttcg
    28801 gattgcgcta aaccggcttc ttcgcgaccc cgtgcgccgg tccagttcgc atatgccaca
    28861 gacgctcgtt gcagcacatc cagttttgcc tgttcgacct gcgaggcaga gattttttgt
    28921 tgcccttcgg cctgttgcac ctgggcagaa accgcgccgc cctgataaag cggcgcgtta
    28981 acgttcagtt gtagctgatc attccaatac gagcggtcgc tggtctggta gcgcgtttta
    29041 cccccctgaa tactgagcgt tggccagtat tgcgctttcg ttttttccac gccgtactgt
    29101 gctgactggc gtaagttttc tgccgccagc accagcggga tggactggta atcaatattc
    29161 ttcagcgata ccggctgctc ggctaattca gcaggtggcg cggctatcgc ctccggctgt
    29221 acgccagtga gcaccgccag ttgcgctttg gcgctcgcca tctgcgcctg atattgctcc
    29281 agcgttgagc gcattccggc aatacgcgtc tgggcctgta attcatccga cgatgagttc
    29341 aggccagcgt tagcgcgcaa tgccgccatg ttgtagacgt tttccagcga gtgaatattg
    29401 cgttgagccg catcgcacaa agcctgataa cgactgactt ccatataggc agtcgccgtt
    29461 ttctctgcaa catcggttaa ggtagccatc aatttaaagc ggtagctgtc acgggcggca
    29521 gtttgcagat tgatatcgtt gttagtttta ccaaagtcat acaccagttg tgtcagggtt
    29581 atgccatacg aaacgttgtt atcaagcctg ccgctggagt ccgtttgtcg ggacggcccc
    29641 gcgttgccgg ttaatccaac gtgcggatac caggcacttt tggcttcatc aatttgcgct
    29701 tcgccaatgc ctatctgcgc cgcctgttga gtgacgtcgg gatttcgcgc aaaggcgcgt
    29761 aatatacttt cctgtaaaga taattgggca actggcgtta ccgctggggc attcgcccat
    29821 tgtggcaacg gtgcagcaat tgcagcttct cccatcacca taaaacttat aaaaaaaata
    29881 agtccattat ttttacggtt tttaaactg
//
LOCUS       DAPGRN010000058        29062 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPGRN010000058 DAPGRN010000000
VERSION     DAPGRN010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.058"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            323..1189
                     /gene="folD"
                     /locus_tag="RHW33_002633"
     CDS             323..1189
                     /gene="folD"
                     /locus_tag="RHW33_002633"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV1646121.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1191..1403
                     /gene="ybcJ"
                     /locus_tag="RHW33_002634"
     CDS             1191..1403
                     /gene="ybcJ"
                     /locus_tag="RHW33_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV1646122.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1511..2032
                     /locus_tag="RHW33_002635"
     CDS             1511..2032
                     /locus_tag="RHW33_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1646123.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGLAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGILWWRRR"
     gene            complement(2068..3453)
                     /gene="cysS"
                     /locus_tag="RHW33_002636"
     CDS             complement(2068..3453)
                     /gene="cysS"
                     /locus_tag="RHW33_002636"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV1646124.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3627..4121
                     /gene="ppiB"
                     /locus_tag="RHW33_002637"
     CDS             3627..4121
                     /gene="ppiB"
                     /locus_tag="RHW33_002637"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV1646125.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4124..4846
                     /gene="lpxH"
                     /locus_tag="RHW33_002638"
     CDS             4124..4846
                     /gene="lpxH"
                     /locus_tag="RHW33_002638"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV1646126.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAGIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRTKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4963..5472
                     /gene="purE"
                     /locus_tag="RHW33_002639"
     CDS             4963..5472
                     /gene="purE"
                     /locus_tag="RHW33_002639"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV1646127.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGID
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5469..6536
                     /gene="purK"
                     /locus_tag="RHW33_002640"
     CDS             5469..6536
                     /gene="purK"
                     /locus_tag="RHW33_002640"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV1646128.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6675..7568)
                     /locus_tag="RHW33_002641"
     CDS             complement(6675..7568)
                     /locus_tag="RHW33_002641"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV1646129.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTDDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7565..8380)
                     /locus_tag="RHW33_002642"
     CDS             complement(7565..8380)
                     /locus_tag="RHW33_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV1646130.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRVINLMTESGELLTLHR
                     KGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDAHSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFAHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8391..9650)
                     /locus_tag="RHW33_002643"
     CDS             complement(8391..9650)
                     /locus_tag="RHW33_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV1646131.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPSWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9660..11327)
                     /locus_tag="RHW33_002644"
     CDS             complement(9660..11327)
                     /locus_tag="RHW33_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV1646132.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDAHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPTLRNQLIADLGAKPSVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11644..12693
                     /gene="allD"
                     /locus_tag="RHW33_002645"
     CDS             11644..12693
                     /gene="allD"
                     /locus_tag="RHW33_002645"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV1646133.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISV
                     ALYNTSYETKNPFAQ"
     gene            12715..13950
                     /gene="allC"
                     /locus_tag="RHW33_002646"
     CDS             12715..13950
                     /gene="allC"
                     /locus_tag="RHW33_002646"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV1646134.1"
                     /translation="MITHFRQAIEEALPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPDAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13961..14746
                     /gene="allE"
                     /locus_tag="RHW33_002647"
     CDS             13961..14746
                     /gene="allE"
                     /locus_tag="RHW33_002647"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV1646135.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14974..16119)
                     /gene="glxK"
                     /locus_tag="RHW33_002648"
     CDS             complement(14974..16119)
                     /gene="glxK"
                     /locus_tag="RHW33_002648"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV1646136.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16141..17442)
                     /locus_tag="RHW33_002649"
     CDS             complement(16141..17442)
                     /locus_tag="RHW33_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV1646137.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQVTFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17499..18860)
                     /gene="allB"
                     /locus_tag="RHW33_002650"
     CDS             complement(17499..18860)
                     /gene="allB"
                     /locus_tag="RHW33_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV1646138.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18920..20374)
                     /locus_tag="RHW33_002651"
     CDS             complement(18920..20374)
                     /locus_tag="RHW33_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV1646139.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20543..21421)
                     /gene="glxR"
                     /locus_tag="RHW33_002652"
     CDS             complement(20543..21421)
                     /gene="glxR"
                     /locus_tag="RHW33_002652"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV1646140.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETAHQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKTLALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21521..22297)
                     /gene="hyi"
                     /locus_tag="RHW33_002653"
     CDS             complement(21521..22297)
                     /gene="hyi"
                     /locus_tag="RHW33_002653"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV1646141.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22310..24091)
                     /gene="gcl"
                     /locus_tag="RHW33_002654"
     CDS             complement(22310..24091)
                     /gene="gcl"
                     /locus_tag="RHW33_002654"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV1646142.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCVADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24181..24996)
                     /gene="allR"
                     /locus_tag="RHW33_002655"
     CDS             complement(24181..24996)
                     /gene="allR"
                     /locus_tag="RHW33_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV1646143.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25074..25556)
                     /gene="allA"
                     /locus_tag="RHW33_002656"
     CDS             complement(25074..25556)
                     /gene="allA"
                     /locus_tag="RHW33_002656"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV1646144.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25786..26712
                     /gene="allS"
                     /locus_tag="RHW33_002657"
     CDS             25786..26712
                     /gene="allS"
                     /locus_tag="RHW33_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV1646145.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     RVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26781..27875
                     /gene="mnmH"
                     /locus_tag="RHW33_002658"
     CDS             26781..27875
                     /gene="mnmH"
                     /locus_tag="RHW33_002658"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV1646146.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVAGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27991..28356
                     /locus_tag="RHW33_002659"
                     /pseudo
     CDS             27991..28356
                     /locus_tag="RHW33_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(28877..29050)
                     /locus_tag="RHW33_002660"
     CDS             complement(28877..29050)
                     /locus_tag="RHW33_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646147.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
BASE COUNT         7158 a         7853 c         7229 g         6822 t
ORIGIN      
        1 gttagatgaa tacttccgcg caaaatatat tcaacacaaa tatagacctg aagcggtaaa
       61 ttaccaggct gaaaattctt tttatattgt caggtatttc ttaaattatc ttaatccttt
      121 gacaaggaaa taagtcagtt ccagatttac aacgccaaca tggacgaaaa atgaagcttt
      181 caatctcagc gacagtgcgc ctcaccttcg caagaggtcg cttcacgcga taaatctgaa
      241 acgaaacctg acagcgcgcc ccgcttctga caaaataggc gcatcccctt cgacctacgt
      301 aacagatgga atcctctctc tgatggcagc aaagattatt gacggtaaaa cgattgcgca
      361 gcaggtgcgc tctgaagttg ctcaaaaagt tcaggcgcgt attgcagccg gactgcgggc
      421 accaggactg gccgttgtgc tggtgggtag taaccctgca tcgcaaattt atgtcgcaag
      481 caaacgcaag gcttgtgaag aagtcgggtt cgtctcccgc tcttacgacc tcccggaaac
      541 caccagcgaa gcggagctgc tggagcttat cgatacgctg aatgccgata acaccatcga
      601 tggcattctg gttcaactgc cgttaccggc gggtattgat aacgtcaaag tgctggaacg
      661 tatacacccg gacaaagacg tagacggttt ccatccttat aacgtcggtc gtctgtgcca
      721 gcgcgcgccg cgtctgcgtc cctgcacccc gcgcggtatt gtcacgctgc ttgagcgtta
      781 caacattgat accttcggcc tcaacgccgt ggtgattggc gcatcgaata tcgttggccg
      841 cccgatgagc atggaactgt tgctcgcagg ttgcaccacc accgtgactc accgtttcac
      901 taaaaatctg cgtcaccacg tagaaaatgc cgatctactg atcgttgccg tcggcaagcc
      961 gggctttatt cctggtgact ggatcaaaga aggcgcaatt gtgattgatg tcggcatcaa
     1021 ccgtctggaa aatggcaaag ttgtgggcga cgtcgtgttt gaagacgcgg ctaaacgcgc
     1081 ctcatacatt acgcctgttc cgggcggcgt tggcccgatg acggttgcca cgctgattga
     1141 aaacacgcta caggcgtgcg ttgaatatca tgatccacag ggtgagtaac atggcgacat
     1201 tttctttagg taaacatccg cacgttgagc tgtgcgactt gctgaaactg gaaggctgga
     1261 gcgaaagcgg cgcgcaggcg aaaatcgcga ttgccgaagg ccaggtgaaa gtcgacggtg
     1321 cggttgaaac gcgcaaacgc tgcaaaatcg tcgccgggca gacagtgagt tttgcaggtc
     1381 acagcgtaca ggttgttgcc tgatcccttt cccggcctgt atcaacaggc cggttctctt
     1441 ttcagacttt tgccctctac gccccgttca tccatgctga tagcatcgac attttgccga
     1501 agcgaccatc atgccaaccg ttattacgca cgccgccgtt cccctatgca ttggtttagg
     1561 gttgggttcg aaagtcatcc ctccgcgtct gttatttgcc ggaatcatcc tggcgatgct
     1621 gcccgacgca gacgtattgt cgtttaaatt tggccttgct tacggcaatg tttttggtca
     1681 tcgcgggttt acccattcgc tggtgtttgc gtttgttgtc ccgctgttat gtgtgctgat
     1741 tggacgccga tggttcaggg ctgggctgat tcgctgctgg ctgtttttaa ccgtctcgtt
     1801 gctatcgcac agcatgctgg attcggtaac cactggcggt aagggtgttg gctggctgtg
     1861 gccgtggtca gatgaacgct ttttcgctcc ctggcaggtg attaaagtcg cgccgtttgc
     1921 attgtctcgt tacaccacag cgtacgggca tcaggtgatt atttctgaat tgatgtgggt
     1981 gtggttgccg gggatgctac tgatgggaat attgtggtgg cgcagacgat aaccggatgc
     2041 gaaaactcgc atccggcaat agcgcaatta cttacgacgc caggtggtcc cttgcgggcc
     2101 atcttccagc acgatcccca tctcgttaag acggtcacgc gccgcatctg ccgccgccca
     2161 gtctttcgct ttacgggcat ccagacgctg ttgaattaac gcttcaatct cagccacttc
     2221 gctgtcatct gcctgcgcgc cgctttgcag gaacgcttcc ggttcttgct ccagcaggcc
     2281 caatacagcg gaaagtttac gcaggtgaga tgccattgca ttcgccgctg ccatatcttc
     2341 tgctttcaga cggttcactt cacgcgccat atcaaacagc acggaatagg cttccggggt
     2401 gttgaaatcg tcgtccatcg cttcaataaa gcgcgcttca aacgcttcgc caccggcagg
     2461 cgcaacggtt ttatctgtgc cgcgcagcgc agtgtagaga cgctccagcg ccgcacgcgc
     2521 ctgcttcagg ttctcttcgc tatagttcag ctggctgcga tagtggcccg acatcaggaa
     2581 gtaacgcacg gtttccgcgt cgtagtattt cagcacatcg cgcacggtaa agaagttacc
     2641 cagcgatttg gacatcttct cgcggtcaac catcaccatt ccggagtgca tccagtagtt
     2701 aacatactga ccatcatggg cacaggtgga ctgcgcgatt tcgttttcgt ggtgcgggaa
     2761 catcaggtct gaaccgccgc cgtggatatc aaagtggtta cccagctgct tgcagttcat
     2821 tgccgaacat tcaatgtgcc agccaggacg acccgcgccc cacggagacg gccagctcgg
     2881 ttcgccctct ttcgacatct tccacagaac gaagtccatc gggttgcgtt tgtcgtcgac
     2941 cacgtcaacg cgcgcgcctg cctgcagctg gtcgagatcc tgacgcgaca gcacgccata
     3001 agttggatcg gttgggacgt cgaacatcac gtcgccgttg tccgccacat aagcgtgacc
     3061 tttggcgatc agttgttcag tgagttcaat aatttctgcg atatggtgcg tcgcgcgcgg
     3121 ctccatatcc gggcgcagaa tgttcaacgc atcaaaatct ttgtgcattt cggcgatcat
     3181 gcgatccacc agcgccacaa agctttcgcc attttcattg gcgcgtttga tgattttgtc
     3241 gtcgatatcg gtaatgttgc gcacatactt cagcttatag ccgaggaaac gcagatagcg
     3301 cgcaaccacg tcaaaagcaa caaaggtacg cccgtgaccg atatgacaga gatcgtaaac
     3361 ggtgattcca cacacgtaca tgccgacttc cccggcgtga ataggcttaa attcctcttt
     3421 ttggcgtgtc agagtattga agatttttag catcgaagat tccgtttaga catgtgtggg
     3481 taattgagtt gcgtataata cccatatttc ccgcgcgaat cagcatacat tgcgtgatga
     3541 tccgatcgtg cggttatgct ataacaccac cctatatatg acccgaaccg ggttgaagca
     3601 ccaatcaaac ggaacaggat gcaaaaatgg ttactttcca caccaatcac ggcgatattg
     3661 tcatcaaaac ttttgacgat aaagcacctg aaacagttaa aaacttcctg gactactgcc
     3721 gcgaaggttt ttacaacaac accattttcc accgtgttat caacggcttt atgattcagg
     3781 gcggcggttt tgaaccgggc atgaaacaaa aagccaccaa agaaccgatc aaaaacgaag
     3841 ccaacaacgg tctgaaaaat acccgtggta cgctggcaat ggcccgtact caggctccgc
     3901 actctgcaac tgcacagttc ttcatcaacg tggttgataa cgacttcctg aacttctctg
     3961 gcgaaagcct gcaaggttgg ggctactgcg tgtttgctga agtggttgaa ggcatggacg
     4021 tggtagacaa aatcaaaggt gttgcaaccg gtcgtagcgg tatgcaccag gacgtgccaa
     4081 aagaagacgt tatcattgaa agcgtgaccg ttagcgagta atcgtggcga cactctttat
     4141 tgcagatctt catctctgcg tggaagaacc ggcgatcacc gccggttttc tgcgtttttt
     4201 agcgggggaa gctcgcaagg ccgacgcgct gtatattctt ggcgatctgt ttgaagcgtg
     4261 gattggcgac gacgatccca atccactcca tcgccagatg gcggcaggga tcaaagcggt
     4321 gtccgattcc ggcgttccct gttatttcat tcatggcaac cgtgattttc tgctcggcaa
     4381 acgctttgcc cgtgaaagcg gcatgacgtt attgccggaa gaaaaggtgc tcgaacttta
     4441 tggtcgtcgg gtgctgatta tgcatggcga cacgctgtgc accgatgacg cgggttatca
     4501 ggcttttcgc accaaagtcc acaaaccctg gctgcaaacg ctattcctcg ctctaccgtt
     4561 gtttgtgcgc aaacgcattg ccgcgcgaat gcgcgcgaac agtaaagaag ccaacagcag
     4621 taaatcgctg gcgatcatgg acgttaacca aaacgcggtg gtcagtgcga tggaaaaaca
     4681 tcaggtgcaa tggctgatcc acgggcatac ccatcgcccg gcggtgcatg aacttatcgc
     4741 caatcagcaa cctgcttttc gcgtggtact gggtgcctgg catacggaag gttcaatggt
     4801 gaaagtcacg gcggatgacg ttgaactgat tcattttccg ttttaaatac ccgcgacttt
     4861 gctgatttca cagccacgca accgttttcc ttgctctctt tccgtgctat tctctgtgcc
     4921 ctctaaagcc gagagttgtg caccacagga gttttaagac gcatgtcttc ccgcaataat
     4981 ccggcgcgtg tcgccatcgt gatggggtcc aaaagcgact gggctaccat gcagttcgcc
     5041 gccgaaatct tcgaaatcct gaatgtcccg caccacgttg aagtggtttc tgctcaccgc
     5101 acccccgata aactgttcag cttcgccgaa agcgccgaag agaacggtta tcaggtgatt
     5161 attgcgggcg caggcggcgc agcgcacctg ccgggtatga ttgccgccaa aacgctggtg
     5221 ccagtgctgg gcgtgccagt acagagcgcc gcactgagcg gtatcgatag cctctactcc
     5281 atcgtacaaa tgccgcgcgg cattccggtg ggtacgctgg cgattggtaa agctggcgcg
     5341 gcaaacgcgg cgttactggc agcacaaatt cttgcgactc atgataaaga gctgcaccag
     5401 cgtctgaatg actggcgcaa agcccagacc gacgaagtgc tggaaaaccc ggacccgcga
     5461 ggtgcggcat gaaacaggtt tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc
     5521 aggcaggcga accgttaggc attgctgtct ggccagtcgg gctggacgct gaaccggcgg
     5581 cggtgccttt tcaacaaagc gtgattaccg ctgagataga acgctggccg gaaaccgcat
     5641 taacccgcga gctggcgcgc catccggcct ttgtgaaccg cgatgtgttc ccgattattg
     5701 ccgaccgtct gactcagaag cagcttttcg ataagctcca cctgccgacc gcaccgtggc
     5761 agttacttgc cgaacgcagc gagtggcctg cggtgtttga tcgtttaggt gagctggcga
     5821 ttgttaagcg tcgcactggt ggctatgacg gacgcggtca atggcgttta cgcgccgatg
     5881 aaaccgagca gttaccggct gaatgctacg gcgaatgtat tgtcgagcaa ggcattaact
     5941 tctctggtga agtatcgctg gttggcgcgc gcggctttga tggcagcacc gtgttttatc
     6001 cgctgacgca taacctgcat caggacggta ttttgcgcac cagcgtcgct ttcccgcagg
     6061 ccaatgcaca gcagcaagcg caagccgaag agatgctttc ggcgattatg caggagctgg
     6121 gctatgtggg cgtgatggcg atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg
     6181 aactggctcc gcgtgtgcat aacagcggtc actggacaca aaacggtgcc agcatcagcc
     6241 agtttgagct acatctgcgg gcgattaccg atctgccgtt accgcaacca gtggtgaata
     6301 atccgtcggt gatgatcaat ctgattggta gcgatgtgaa ttatgactgg ctgaaactgc
     6361 cgctggtgca tctgcactgg tacgacaaag aagtccgtcc gggccgtaaa gtggggcatc
     6421 tgaatttgac cgacagcgac acatcgcgtc tgtccgcgac gctggaagcc ttgatcccgc
     6481 tgctgccgcc ggaatatgcc agcggcgtga tgtgggcgca gagtaagttc agttaacctg
     6541 gtgctcattt gattgccgga tgcggcgtga acgccttatc cggcctacac cgatccggta
     6601 cccattgtag gcctgataag acgcgtcaag cgtcgcatca ggcatggtgc accgattgct
     6661 ggatgctctc atcactacag cgaaatacag gtccccgctt cgcccgccag cgtctcttca
     6721 attcgcgata acgccccaat ccacgcgggt ttaccacggc ttctgacata accactcacc
     6781 gccgttacct tcggccccat cgaaccatcg gctttggcaa atggcgctaa ctcatccggt
     6841 gtggcatggc gaatggcgcg ttgctgcggc gttccccagt tttcatatac cgcatcagca
     6901 tcggtgagga tcaccagtcc atcggcgtta atttgctcgg cgagcaacgc agcggcgaga
     6961 tctttatcaa tcactgcttc actccctgct ccgtcatccg tcacaggcac accgccgccg
     7021 ccactgcaaa tcaccacatg cccctctttg agcaacaact cgatggcttc gctgtcgaga
     7081 attttacgcg gttgcggaga cgccaccacc cggcgcaaat atttaccatc acgtttcatc
     7141 tgccagccgt aagccgcttc cagtgcctct tgttcttctg gctgataaac cggaccaata
     7201 aatttctctg gctgcaaaaa cgccggatca tcaggcgaaa cctcaatgcg cgtcagcacc
     7261 gtcgtgacag gcggcatctg cggctgtgcg ctcaagctct gcgccagcat atagccaatc
     7321 atcccctggc tttccgccac caacacatcc agcggatacg gttcgacctc tttccacgcc
     7381 agattctgta acgccagcag ccccacctgc ggcccgttgc cgtgaacaat cgccagccga
     7441 taagaacggg ccaggcgtgc cagcgcgggt acagcactgg cgatattgcg atattgattt
     7501 tctgccgtca gcgcctcacc gcgctggagt aaggcgttgc ccccaagagc tacaaccagt
     7561 gttttcatgg ttttccttgt aataattgtt gaccaagcca gaaccccagc agcgtatctg
     7621 cccctgacgt atgccccagc gccagcagcg aatcaatcgc aacagcggta cgtttcgggc
     7681 aactcagagc atgagcaaag tgcaggagtg gcgaggcgaa atatccttgc gcggcataac
     7741 gtaaataact gacgctcacc gctgtggtaa cgagttgaag attgtcggaa caggcaaaaa
     7801 actggcgacc ggagtgcgca tctaaagcac cataatacca ggccgccagc agcattccgc
     7861 tcagcgtgtc atcatgactt ggcgttaatc cggggccttt acccagccag tgccgccagt
     7921 cggtcttaac gccattgagc gcggcctgaa aacagtgacg aaactggcgt aactcagcag
     7981 gcagcggatc gcttgccgcc aacgccagtg gcccgaaaag cccggtttcc tccgcgcgtt
     8041 gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg cgtaatacgc agcaaacaat
     8101 aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat cccttgagcc acaacctgtg
     8161 gtcgttcatt gccgcataat ccaccgcata acgcatcgaa ttgcgcacgg cgaagcaccc
     8221 atcctccggg gccgaaacca ctacccttac ggtgcagcgt taacagttcg ccgctttccg
     8281 tcatcaggtt aatcacccgc cgccacactc ccgctaaccg ccaggactgg cgataattcg
     8341 gtgcgctacg gctggcaagc agaggatgga tgatcgtcat tgcgcgttcc tcaaccaatc
     8401 cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac acgccagcgg tgcccgcaca
     8461 gtgcctgcgc caatctgccc gatccccgcc tctttatggg cgataccggt attgatgagt
     8521 ggcgtaatgc cggtttctac cacgcgacga atatccagcc ccaggcacgc gccctggaaa
     8581 tcccagcttg ggatctgcaa ctgcatattg cgttcaaggt aaatttccgc catctcttca
     8641 gataccgccc tggccgcttc catgccaccc gcaccgacaa agcgcgttac gccaggcgct
     8701 gcgatcattg ccgcacctcc gataccaaag gtttcggtaa tcgcgctatc gcccatatcc
     8761 gggttcgcct gctcctgcga gaagccggtg aaaaacagac cttgcggagt gtttacaggc
     8821 gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc cgaacatatt gccgttgcgg
     8881 gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc cagcatccat cgccgccttg
     8941 cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca cgctgaggaa atccatcact
     9001 tccgcgatgt gctgtttatc atgatcgagg cgagcaattt gtggggccag cgcacgcatt
     9061 aacagtgctg aggaagcaat attgcgttga tggaactcat cgcccatggt aatgccctgc
     9121 gccatcatcg cggtaagatc gataccgcgc tccatgcgcc ccagcgccgc gcttaatact
     9181 ggcattaaca catcgcgcat ccagcgatgg cgagtcagga catcttcgcc gtaagcgcca
     9241 aaacgcatca ctttgccgat accttcgttg aggttgcagt acgcccggtt gccgtcggtc
     9301 acgttctcga ccaccagcat cggcatactg gcagaagtaa taccgcccat tggcccgacg
     9361 gcattcacat ggtgacaagg aatgaagttc acttccccct gctccagtat tgccagcgcc
     9421 tgcgcttcat ctttcgccca accttcgaac agacatgcgc ccacgcacgc ccctttcatg
     9481 ggtccggtca tctcctgcca gcgcattggc ggcccggcgt gaagcagtgt tttgccctcg
     9541 tttagttcgc tgataagtga agaagccggt tgcacatcca gccagtgtgg acgagcgcga
     9601 cgaatttgtt cgataaccgc agcattggct tgcgccactg atgtaaacat gggaacccct
     9661 tattgcaaac gttctaataa acgagccagt tttttattgc cgccggcgac tggcgaccat
     9721 tggtaatgca caaccggttt gctggcgctt tgtagctcca gcgcaaaact gcgtaatccg
     9781 atgttaatca cggcgacgtt ttccagtaat gacggtgtgt gttgctgtgc ggcaggcgag
     9841 agcggatgaa ttaacgctgc cgccagcaag gtggcttccg gtagcgaact cacgaccgca
     9901 atccccgcat cttccagcgt ggcgatttgc tgcgagcggc attgcgggtc acgttcggta
     9961 cctgtcaccg tggcaatggc atacagtggt tgattgtctg aacgcgcggc acaggctttt
    10021 tgccaggcgc tcaccagcga ggcggcagga tcggcggtcg cgccgaagcc aatcacgaca
    10081 tcaagtagca atacgcgcac tgacggttta gcgccgagat cggcaattaa ctggttgcgt
    10141 aaggttgggt cgatcatcgg atggggacgc ccgactgtgt agaaatcatc gccaaggtca
    10201 ataatctggt gaccatcggc gtcgagcatc atgccgtgat gatgggcatc atcggcttcc
    10261 acgccaaggt gtccggcaag taatcccgcc gcttcggcag ccagcgtacc gccggtatac
    10321 aaaccgcaaa taaatccgct gctggcaggc gttatcgcgt tacgtcgcgc cgtaacgcgt
    10381 gaaagcaggc aagccaggcg tgcggcctca tccagcgagg aggcaaacca gacattctcg
    10441 tcgcgggcca ccgccggggt ataacctaaa aacagcgcca ccgtcggttt gccggttgct
    10501 ttcatggcat taacaatttt cagacgcaca gcttcggcag gtggttttga aacaaatgcc
    10561 agcacttcgc ttttctcgtc tgcactgagc atttccagcg ctgttagcgc actgatgccg
    10621 cccacttcac ggctgaggtc gcggccgcca aggccaatcg cgtgagtaat tccctcccct
    10681 gccagcgcaa tctgcgagca cagctcctga atcccggtac cggaagcgcc aatgacgcca
    10741 atgttgcctt ccggcatcac gttagcaaaa gccagcggcg tgccggcaat catcgacgta
    10801 ccgcagtccg gccccatcac cagcaagcct ttttcccgcg cgcgggtttt aagttggatt
    10861 tcatcttcca gcgtgacgtt atcggagaac atcatcacgt tgaggttgcg atccagcgcc
    10921 tgattcgcca gctccgccgc atactcgcca gccactgaaa tcagcgccag attggcatcg
    10981 ggtaatttct gacaggcact gtcccagcga cgcacctgcg tcaaggactg gctgctgcct
    11041 gacccctgcg ccagttgttt tagcgcctct tcaagctgct gcataatcgc ctgcgcgatc
    11101 cccgcatccg ccgcttcgct acgaattgcc acgcaaatat cgttcggcgt ggcgttatta
    11161 aaatcgtcat gccagaaacc tgtggtatct aataacgctt tattggcggg cgtacccatc
    11221 attacggaaa catcatcaac attttctgat tcgctgagtt ttcgtgaaat aatcattaaa
    11281 ctgaccgaat cctgaaaaca ccctttttta ataaaggcgt ggatcataac aactccttaa
    11341 aaattgcacc catggcaatt gcacgagcag tgttaatgtg gcgattacgg tatcgatgac
    11401 aacgggtaaa taaggtgatg aatatcacac agggattatt aacaaaagaa ttcaaataac
    11461 cgccatgcta ttacgtgtta ttttttaata agcgtggttg ttttaaaaac caggttaaaa
    11521 gcgagcggat tatcaaataa attctaacta tatttttttg cctgtctgga tcacataatc
    11581 cagatatttt ccctgtatgt taattgcagt catgcttcac accgtcgtta aaaaggaaga
    11641 cagatgaaaa tcagtcggga aacactccac cagctaattg agaataaact ctgccaggct
    11701 gggttaaaac gtgagcacgc tgcaaccgtg gctgaagtat tggtttacgc cgatgccaga
    11761 gggatccact ctcatggcgc ggtgcgcgtg gaatactacg cggaacgcat ttcaaaaggc
    11821 ggcaccaacc gcgaaccgga gtttcgtctt gaggaaaccg ggccgtgctc ggcaatttta
    11881 catgccgaca atgccgccgg acaggtcgcg gcgaaaatgg gtatggaaca tgccatcaaa
    11941 accgcccagc aaaatggcgt tgcggtggtc ggtatcagcc ggatgggtca cagcggcgca
    12001 atctcttatt ttgtgcagca ggcagcccgc gccggattaa ttggcatttc gatgtgccag
    12061 tccgatccaa tggtggtgcc gtttggcggc gcggaaattt actacggtac taacccactg
    12121 gcctttgccg cgccgggaga aggcgacgag atccttacct ttgatatggc gactaccgta
    12181 caggcatggg gaaaagtgct cgacgcccgc tcgcgtaata tgtctatccc ggatacctgg
    12241 gcggtcgata aaaacggtgc accaacaacc gatccgttcg cggtacatgc tctgctcccc
    12301 gcagccgggc cgaaagggta tggcctgatg atgatgattg acgtcctctc aggcgtctta
    12361 ctcggcttac cgttcgggcg acaggttagt tcgatgtatg acgatttaca cgccgggcgt
    12421 aatttggggc aattacatgt agttattaac ccgaactttt tctcctccag cgaattattc
    12481 cgtcaacatc ttagccagac catgcgcgaa ttaaatgcca ttacccccgc gcccggtttt
    12541 aatcaggttt attatcccgg acaggatcag gatattaaac aacgcaaagc cgccgtcgaa
    12601 ggcatcgaaa ttgttgatga tatttaccag tatttaattt ccgtcgcgct ttataacacg
    12661 tcatacgaaa cgaaaaatcc ctttgcgcaa taattattga gacaggactt tcttatgatt
    12721 acacattttc gtcaagccat agaagaagcg ctgccctggc tttcctcatt tggcgctgac
    12781 ccaacgggtg ggatgacccg tttactttat tcgccggaat ggctggaaac ccagcagcaa
    12841 tttaaaaaaa gaatggccgc aagtgggctg gaaacacgtt tcgatgaagt ggggaattta
    12901 tacggtcgcc tgagtggcac cgaatatcca caggaagtgg ttctgagcgg ttcgcatatc
    12961 gataccgtgg ttaacggcgg taacctcgac gggcaattcg gcgcgctggc ggcgtggctg
    13021 gcaattgact ggctgaaaac tcaatacggc gcaccgctgc gtacggttga agtggtggcg
    13081 atggcggaag aagaaggcag ccgcttcccg tatgttttct ggggcagtaa aaatatcttt
    13141 gggctggcga atcctgacga cgtgcggaat atctgtgatg ccaaaggaaa tagttttgtc
    13201 gatgcgatga aggcttgcgg atttactctg ccggacgccc cgctaactcc gcgtcaggat
    13261 attaaagcct ttgtcgaact gcatatcgaa cagggctgtg tgctggaaag taatgggcaa
    13321 tcaattggcg tggtgaatgc aattgtcggg caacgtcgct atacggtgac gctgaacggc
    13381 gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc gtgatacggt ttacgctttc
    13441 agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga tgggcgatcc gctggttctg
    13501 acctttggga aagtagagcc gcgcccgaat acggtgaatg tggtgccggg taaaaccacg
    13561 ttcaccattg attgtcgtca taccgacgcc gccgtgctgc gtgatttcac ccaacagtta
    13621 gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg gtattgatat cgatttatgg
    13681 atggacgaag aacccgtgcc gatgaataag gacctggtcg ccaccctgac agaattgtgt
    13741 gaaagtgaaa aactgaatta ccgggtgatg cacagtggtg ccgggcacga cgcgcaaatt
    13801 ttcgcgcctc gcgtaccgac ctgcatgatt ttcattccca gcatcaatgg gatcagccat
    13861 aacccggcgg aacgcaccaa tattaccgac cttgccgaag gggtcaaaac gttggcactc
    13921 atgctttatc aacttgcctg gcagaaataa ggagtcataa atgggatatt taaataacgt
    13981 caccggttac cgcgatgatt tactggctaa ccgtgcgatt gtgaaacacg gtaatttcgc
    14041 actgttaaca ccagacggtc tggtaaaaaa tattattccg ggctttgaaa actgtgacgc
    14101 gacaatcctc tccacgccaa agctgggtgc ctcttttgtc gattatctgg tcacactgca
    14161 tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc attgaaacgt tcctgtatgt
    14221 gatctctgga aatatcactg ccaaagccga aggcaaaaca tttgccttaa gcgaaggtgg
    14281 ctatctttat tgcccgccag gctccttaat gacgtttgtt aacgcccagg ccgaagacag
    14341 ccaaatcttt ttatataagc gccgctatgt tccggtagaa ggccatgcac cgtggctggt
    14401 ttctggcaat gccagcgaac tggaacgcat tcattatgaa ggcatggacg atgttattct
    14461 gctggatttt ctgcccaaag agttaggttt tgatatgaac atgcatatcc tctcttttgc
    14521 accaggtgcc agccatggtt atatcgaaac acacgttcag gaacacggtg cctatattct
    14581 ttccggtcag ggggtttata acctcgacaa taactggatc ccggtgaaaa aaggcgatta
    14641 catctttatg ggcgcttatt ctttacaggc aggttatggc gtagggcgtg gtgaagcgtt
    14701 cagctatatt tactcgaaag attgcaaccg cgacgtagag atttaagtca taccactcgc
    14761 ctgattattt ttagctatgt tggattttgc cacggtttat accgtggctt tttttgtaac
    14821 attgcctgat gcgcttcgct tatcaggcct acatgatctc tgcaatatat agaatttgcg
    14881 cgattttcag gaaggatata gtactcatgc cgcatccggc atgagtactg cgctcttata
    14941 cgaaataaat agcatccgct ttaaaggcaa gagtcagttt ttaattccct gacctatttt
    15001 aatggcgcag gcaatatttc gcgcgctgtt gaagagattg gtttcaccgc tggcgagcac
    15061 ttcggctaaa ggtgccagac gtggcaaaat gctgaatacc gcatcaatgc cgtactggtg
    15121 caccacttcc acgccatcac ccaatacgcc agcaatccca atcaccggta cattaaactg
    15181 cttcgccacc gacgcaacgc ccagcggcgc tttaccgcct gccgtttgcg agtcgatgcg
    15241 cccttccccg gtgatcacca gcgccgcgcc ctgcaccgcc tgctccaggt tgaccgcccg
    15301 cagtacaatc tcaatacccg gtttaatatc cgcattgaga aataccgccg ccgcaatacc
    15361 cataccgccc gcagcgccgc cgcccgccat ctgacagaca ttaatttcag tttgctgttg
    15421 taaaacacgg gcgtaatttt gcagcccctg ttccagctct tcgaccatct ccggcgttgc
    15481 ccctttttgc gggccaaaca ccgccgccgc gccgcgtgcc cctaccagcg ggttatcaac
    15541 gtcacatgct acttcaatat ggctgttcgc cagacgcgga tcgcattcct ccatctcaat
    15601 gcttgccacg cgcgctaaat tgccgccgtt agctgccagc acctgaccgt cggcatcaag
    15661 gaaacgcaca ccaagcgcct gcgccatgcc cataccgccg tcgaccgtcg cactgccgcc
    15721 aatgccgaga ataatatggc gaatgccgtt atccagcgca tgacgaatta actcccccgt
    15781 accaaaactg gaggccagca acggattacg cttttcaggc gcgaccagca tcaggccact
    15841 tgccgccgcc atctcaatca ccgccgtttt cccgtcgccg gtaaggccat aaaaagcatt
    15901 cactttttcg cccatcggcc cgcagacttc aagcgtcacg atgttgccgc ccgtcgcggc
    15961 gaccatcgca tccaccgtcc cttcgccgcc atccgctatc ggcaaacaga tatagttcgc
    16021 atcgggaaag agggtcgaaa acccggcttt aattgcctga caacattttt ctgcacttaa
    16081 gctctcttta aaagagtctg gcgcaatgac aatcttcatt atcaatcctt tttcaacaaa
    16141 ttattcgatg cgttcccacg gaataaggtt atccataaga acagccagta aaatcccgac
    16201 caataagccg ttactgagca gaggacgaag cgttaatgga aggtcttgca gatacacagg
    16261 cggtaatgcc attaaaaata tgccgacaaa taacggcaat gcgagtcgat aaatattgcg
    16321 agcggtaaac gttacttgct ggctaaacac cagcgcggaa aagagtaaag gcagataaga
    16381 aaccagcatg accgcactac tcacgggtaa agggatactg caaaacagtc gcgtgagtgc
    16441 cggaaccagc gccaccagca ggcaaataac gctgccataa ataaacgaac gccgcgtgta
    16501 atcgccagtc tgggttaata aaccaatgga tgaaacaaac ggtgaaaatg gaattactgc
    16561 caacggcacg gttatcagtg tcataaatcc ggtcgccaca aagctacgac gataacgcgt
    16621 attccccgcg ccctgctgcg gataaaaaac atccgtgccc cgaatcgcac cgtaggtatt
    16681 gctgatattt accagacctg taatcaccgc cgtcaaaata atccccggcg acaaagcacc
    16741 gccactgccg agcgggaacc actgccagtg caactcaccg gagagcgaat gcgaagaagg
    16801 aaagcaaaag taccacaaca accagccggt tatcgtaccg accagcaggc cataacgggc
    16861 aaaacgttgc ggcaggaaaa taatcatcgc cagtaccagg cacatcaccg ccaccgagag
    16921 cgcgaacggc ggtaactgaa ttttaaaatt cgggtcggct atgccaaacg gcagcccgag
    16981 catacctttg aaaaagatag tggtcagctg cgcgcccagc atcaacataa acaagaccat
    17041 caccgacggc gtaaataacc gtgccaggcg atggcctaat ccgctaaaac caatcaacat
    17101 cgtcagcacg ccggagagcg caatccccac tgccaggctg gtggcgatat cgttgatcgg
    17161 tgtcccgcgc gatgcttcgc caagggtgat agttaggatg gttccccacc acaggccccc
    17221 cggcccttcc ataatagcgc gacgatggcc acagaacgcc tgagcgaagc aggccagtgc
    17281 ggtagcaaga aaagcatatt gtgtgagtgt aagcaggcta ctttgcggca actgaaaagc
    17341 agaaagtagc gtaggaggaa ccacaaccgt gttgcaaaaa ataaagaaaa accactgaaa
    17401 tcctgataac aggctttcgc ggctgactgc aaaattgaac ataaacacac cttaaccgga
    17461 gaatgcccgc cgcaaggacg ggcattgcag gggccagatt actgctgatg tttaaggata
    17521 aattgacctt tcggcgcaac agggaagccc tgttcaatgt cgtaaatcac atcaccacgt
    17581 aagatggttt tcgtgatacg cgcgccaatg gtacggccaa catacgggct gactttgtgg
    17641 cgatattcca gatcgtcatt ggtaagaaca tagctgctat tcggctgaat gaagacgaag
    17701 tcggcatctt ttcctggggc gatacggcct ttttgctgca gaccaaaaat atctgctgcg
    17761 ttagtcgcca ttaatttgcc gaacattggc agagacattc cgcgtttctg taccgcttca
    17821 tcgaacatca cgtccataca gttttgcaga ccggcaatac cgccccatgc ttccatgatg
    17881 ttgccggctt tcatttccgg agggcatggt gagtggtcgg aaaccaggca gtcgatttca
    17941 ccgttaaaca gtttttccca catgcctttc tggttttcca gatcgcggat cggcggtgaa
    18001 cacttcgcca gagtaccgat ttcttcgaac tgatcggtat ccagtacaaa gtaatgcggg
    18061 caggattcac aagtaacgtc ctgaccttcc tgacgtgcac gagtcacttc ttcaacgcct
    18121 tccgggctgc tgatatggca aacgtgcaga cggcaaccgg caactttcgc cagatacagc
    18181 acgcggcgaa tcgcttccac ttcggtaaat accggacgcg aagccacata gtcatgggca
    18241 gttacgcgac cttcacgttt cgcttcttcg cccagtgcgt cacagatcag cgcgttttcg
    18301 cagtgcacca gcaccggctg ccccagttcg cccagcttct gcgcaccttt gaagaactgc
    18361 cagtcgttta catcacggaa gtcgttgtcg ataccgcgat cgccacaggt cgcaacgaag
    18421 catttgaagc cgacaacgcc cacttcatcc agctcatgca gacggtcgat gttgtaagac
    18481 accaggccac cgagttgtgc cgcatcgata gtcagcttgc ctttagcggc atcgaacttc
    18541 agttcaattg aagcgcggtc aaccgttgca ggcagctggt tgagcggcat ttcgatcatg
    18601 gtggtgatac caccttttgc cgctgcgcga gtaccggttt cataaccttc ccagtggcta
    18661 cgacccggtt cagaaatatg ggtgtgcgca tcaaccatgc ccggcgaaac caccagacca
    18721 gacgcatcca taacttcttt tgcatcgccc agatcctgac cgatagcagc aattttgccg
    18781 cctttaacgg cgatatctac aacgcgagct tcgttttcta aaataacggt gccgttttta
    18841 atgattaaat caaaagacat aacaaactcc ttgtaaaata cagattggtt tttaactcaa
    18901 tgttcaatat cgggattaat taacctatgg ttttttgctc tcctgttttt tctgctgttg
    18961 tacgtttctt taataaggcg taggccgcaa aggcgacgat gacgccgaca aaccatgaaa
    19021 cacgcgataa cggttccata aagggaataa acttaccgcc aagagataaa ataacggcca
    19081 ccagagttac tgaaaacgcg gtgaggttaa aaccgttatc gtaatattta taatcgccag
    19141 gtgcggtgta cagttcatca agattaattt gcccacgcat caccacaaaa taatgcgcca
    19201 tcatgacacc aattaccgga cccagcattc cgccgatgat atcgaggaac agataaatac
    19261 tgtcctgatt ttccattaat ttccacgggc agatcagcag gctgataata ctggcaatca
    19321 gtacgccgtt tttataggtc agttttgtcg gtgcaatggc agcaatctga taaccggccg
    19381 gaataatatt accggtggcg ttagtggaaa tggtcgtcat cagaataacc agtaccgcaa
    19441 agaacgaggc aaacaggctg tcccaacgct gaacaatatc cagcacgttc caggtgtcag
    19501 cgccgtagtg aatactggct ccggcaataa tacatacccc agcgaccgca aacagaatat
    19561 aggccacaac taaacccagc gtttgcccca gcgcttgctc acgaaacgag tgggcgtttt
    19621 gcgtaaagtc ggatgcgctc accgccggtg ccgcccagac cgcaactacc gcgttaatca
    19681 ccaccaggaa caagaatcca ctgttttctg ctttctgaat accgctcgga atgtagtcaa
    19741 agattggacc gagcccgacc agcgaaatcg cccaaatcgc cataccgccg aaaacgatat
    19801 agatgcacgg gttaagaatg gcagtgaatt tatttaaaac tttgccaccg ccaaaaccta
    19861 taccaacgtt gaccagccag aagagtaaga aagtaattaa gcccggtaga gaaaggccta
    19921 acagagtgaa atcaccaccg agagtcaaaa atcccggcca gattttgcca atcagaatca
    19981 agcaggccag tgaccccgcg taacattgca ggccaaacca cataatggca gcaataccgc
    20041 ctcttaataa tccgggaaac agtgcgccac gtacgccgta agaagcacgc aggatcatgg
    20101 caaacggtac gccgtattta ctgcccgcag cgccgtttaa taccattacc gcggcaatga
    20161 aaaaggcgct gaggataatt gccagcataa tactaaaggt agacaagccg agaataaaaa
    20221 agccgccgac catcacataa ttgggaacgt tatgaaccga acccatccat aaggtaaaat
    20281 agttaaatgt tttccaggtc cgctggcttt gcgttttcgg caatagatct tcgctatagc
    20341 cgcgttgctg gaatagtttt ctctgatgtt ccataaatcc ctcttaattt cacgtatcga
    20401 ataacgttgt tttaatctag ctatgaggaa caaataaaat aaaaatatta tttttaatac
    20461 tgaagcaaca tctttggctg ctttcttttt taatattgct ggccgattca ttttctgata
    20521 tcggccattt ttattgcggg tatcaggcca gtttatggtt agccattaat tccagcgcct
    20581 gcactaacgc agagtgatcc aactggctgc caccgtttgc cgcacaggta ttaaataact
    20641 cctggcaggt cgcggtgttt ggcagattca gcgccagtgt cttcgcactt tgcagtgcca
    20701 ggttgagatc tttctggtgc agcgcaattt tgaaacctgg attaaaggtg cgtttaatca
    20761 tgcgctcacc gtgaacttcc agaatacgtg aggaagcaaa gccgcccatc agcgcctggc
    20821 gcacacgtac cgggtctgca ccggcttttg aagcaaacag cagagcttca gagaccgctt
    20881 caatattgag cgctacgata atctgatttg ccactttgca ggtttgaccg tcgccgttac
    20941 cgcccacaag ggtgatattt ttaccgagca gttcaaacag cggtttaaca cgttcaaata
    21001 ccgcttcgtc accgccaacc ataattgaca acgtcccttc acgcgcgccg atttcgccgc
    21061 cggagactgg cgcatcgaga taatcaccgc ccagttcatt cacctgacga gcgaaacgtt
    21121 tagtttcaat cggggaaatg gagctcatat caacaatggt tttgcccttc agcgaggctt
    21181 tggtacaacc attttcacca aacagaactt cttcaacctg tggtgtatcc ggcaccataa
    21241 taaaaatgat gtccgatgct tccgttacct ggtgcgcagt ttcaacactg acggcaccca
    21301 gtgacagtaa ttcatcagca accggaccaa tggttgtgac atgcagttga tgaccggcac
    21361 gcgccagatt aatggccatc ggtgtaccca taatgcctaa gccaataaat cccagtttca
    21421 taattaacct cttttaaatt ttgcttttcc tggattcaga caacacgatc ccctgccaac
    21481 ttagcggcct aaaaaagcat tgcctgaaca gcgttacgtt ttaacggtac ggatccatcc
    21541 agcgtaaacc ggcttccgtg gtggtttgtg gtttatattc acacccaacc caaccgttgt
    21601 agtcagaatt ttcaattacc gtaaagagat attcgtaatt aatttctccg gttcccggtt
    21661 cgccgcgatg cggattatcg gcaatttgca ggtgaccaat tttatcggcc cactgagtca
    21721 tggtgttggt taattcacct tccatccgct gcatgtgata aatgtcatac tgaattttca
    21781 aattgcagca accaacatca tcaatcaatt tcagcgcctg ccgagttccg gtgagatgga
    21841 aaccaggaat atcaaaatgg ttaataggtt caatcagtaa taaaatatct tctttcatca
    21901 acatattcgc ggcataacgc aggttttcta caagcgttgc gtgaatctgt tcactgctga
    21961 aaccagccgg cgttttaccg accagacagt taattttttt attacccagc gcacgggcat
    22021 aacgaatcgc tgctgctacg ccatcccgaa actcttcttc acggccagga atacaggcaa
    22081 taccgcgctc ccccgccgcc cagtcacccg ccggtaaatt gtgcagcgta tgttcgagtt
    22141 tattactcgc cagcacctgt tttaattctt caatgtcgta gtcataagga aacataaatt
    22201 caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac
    22261 caaataacat cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt
    22321 gcatgaagca ggtttcagtc ggtgcatccg ctgcgttatc ggcgatatct tcaaattcca
    22381 tgacgttatc cagttcgctg cccatcgaaa tattggtcac acgctcgaga ataacttcca
    22441 cgactaccgg tacccgatat tgcgccatta aggctttcgc ctgttcaaag gccggtgcaa
    22501 tatcttccgg tttgaagacg cgaatcgctt tacaacctaa accttccgct acttttacgt
    22561 ggtcgacgcc gtaaccgttc acttcgctgg agttgatatt ctcgaaagcg agttgcacgc
    22621 agtagtccat gtcaaaggcg cgttgtgact gacgaatcag gccgagatag gcgttattca
    22681 ccagcacatg gatgtacgga atgttgaact gcgcgccaac ggctaactct tcaatcagga
    22741 actggaagtc gaagtcaccg gaaatcgcca ccacattgcg tttcggatca gcgacacaaa
    22801 cccccagcgc cgccggaatc gtccagccta acggaccagc ctgaccacag ttgatccagt
    22861 ggcggtcttt aaagacatgc agcatttgtg ccgcagcgat ttgtgacagg ccaatggtgg
    22921 tgacataaca aacatcgcga ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt
    22981 tcaccggcac gttgtcgaaa tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt
    23041 cggcgaccca ttctttacga cacggcagac gacccgcttt ttgcatctcc tgcgccactt
    23101 caaccagcag tgtcagcgcc gctttagcat cagagacaat gccgagatcc ggacacagca
    23161 cgcggccaat ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct tcggtgtatt
    23221 tctctaccga accggtatga cggttagcaa aacggttacc gataccaaac accatgtcgg
    23281 acgccagcaa cgttgcgtta ccgtaacgat gcgcggtttg cagaccgacc atcccggcca
    23341 tcagttcatg atcgtccggg atacagcccc agcccatcag tgttgggatc accggaacgc
    23401 tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc agcattgatt accccgcccc
    23461 cggcaacaat caccggacgt tcggcctgga ttaacatttc tacagctttt tcgatctgca
    23521 tacggctggc agcaggtttg tagaccggca gcggttcgta catgtcagga tcaaactcga
    23581 tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac cggacccgga cgaccagaac
    23641 gcatcaggtg aaatgcctgt tgcagcacgc gaggcaccag cgccgcttca cgtactgtaa
    23701 ccgccatttt gctgaccggt ttagcaattg cttcaatatc tacggcctga aaatcttctt
    23761 tatgcagacg ggcgcgcgga gcctggccgg taatgcacag aataggaatg gaatcagcag
    23821 aagcggaata gagcgcggtg atcatgtccg tgcccgcagg accggaagtc cccagacata
    23881 cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca
    23941 catgacgcgc cagaatgtga cgaataccgc cgtgcttacg catcgctgag tagaacggat
    24001 tgattgcagc tcccggaaca ccgaaggcgg tggtgatacc ttctttctcc agcacataca
    24061 ttgccgcgtc aacggctctc atttttgcca ttttattcct acctctattt attggaaaat
    24121 ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat gcgacagaca
    24181 ttatggatgt gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag
    24241 ctcaccctga ctgacaaaac ggtcttctgt cagtcttgat gaaggcccgg agatggagat
    24301 agcggcaaca acactaccta catcatcgta aattgctgaa gctatgcaat tcagacctac
    24361 aacatgctct tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag
    24421 caaggtgggc atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat
    24481 gatgctcatc aactcctctt ccgccagcgg ataaagcagc gctttgcccg caccggaagc
    24541 atgcagtggc agacgactgc ccagtggcgc gcacatcctg accatcgatt tacactctaa
    24601 ctgaccaatt aataccgctt cattgccgtt acggatcgcg acattgaccg tttcgccgga
    24661 aagtaacatc aggcggcgca taaacggccc ggcgacggag aggacatcgc ggttatggat
    24721 gtacgctgca ccgacgttaa agacacctaa tcctatatgc caccagccta actgactgtc
    24781 ctgatagaca aaatccgctg cctgtaaaac cttcagcaag cgaaaggtcg tggagagcgg
    24841 caaatccaga ttgagagaaa tatcgctaac cgacgaactt cccccacttt tttccagata
    24901 ttgcaaaatc gcaattcccc gctctaatgc ctgtgcgccc ttctgtgcca caggctccgc
    24961 ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa
    25021 gtgagcataa acaatgaaca attagtttat cttatgcaag tcggttgcag gcgtcacgca
    25081 aaacagagtt cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg
    25141 atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga
    25201 tggtaattca ctccctgttc gccgttggtg ataaacgccc gcagcgttga caggtctggt
    25261 ttgtcgtcac ctaacgccac gaccaccaca aacacctcac ctttcatcgg gataaaggcc
    25321 tgagtaccca gcggatgacg ttcgagttcg tgaatggtca gcggcagatt cgccggttgc
    25381 gcgcggttaa tgctgataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg
    25441 tggtaacgct ccaccaggcc attgttaata tggaaaaaat cccgttgctg cgtttcgatt
    25501 acgtcgccat aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt
    25561 ttgtccttgt aacataattc cgttcaacca gcgccgaccg gtcagcttgg tttcaataac
    25621 aggcaatcta aaaccttaag agaacaacat caacacaaaa gttggaatat ttttccaaaa
    25681 ctagatttag tccacaaatt gaacagggtt atcatgtaaa aatactgata aatcagagga
    25741 taagaaaatc acgcaacttg cggatttaaa aggggatacc tgaagatgtt cgatccagaa
    25801 accttgcgga ctttcattgc ggttgctgaa acaggaagtt tttcaaaagc ggcagaacga
    25861 ttatgtaaaa ccacggcgac gatcagttat cgcattaaac ttctggaaga gaataccaga
    25921 gtagcgctgt ttttccgtac gactcgtagc gtaacgttga cagcggctgg cgagcatcta
    25981 ctttcccagg ccagagactg gctgagctgg ctggaaagta tgcccagcga gctgcaacag
    26041 gtgaatgatg gcgtggaacg ccaggtgaat attgtcatca acaacctgct ctataacccc
    26101 caggccgtcg cccagttgct ggcgtggctg aatgagcgtt acccctttac ccagtttcac
    26161 atctcccgac aaatctatat gggcgtctgg gactcgctat tgtacgaagg tttttcgctg
    26221 gctatcggcg tcacgggaac tgaggcgctg gcaaatacct ttagtcttga tcccttagga
    26281 tcggtgcaat ggcgctttgt catggcggcg gatcatccgc tagcgaacgt tgaagagccg
    26341 ctaacagaag cgcagttgcg gcgctttccg gcggtcaata ttgaagacag cgcccgcacc
    26401 ttaaccaaac gcgtcgcctg gcgattgcca gggcaaaaag agattattgt tcctgatatg
    26461 gaaacgaaaa tcgccgccca tctggcgggc gttggcattg gttttttgcc aaaatcgctt
    26521 tgccagtcaa tgatcgataa tcaacaactg gtcagccggg taatcccaac gatgcgccct
    26581 ccttcgccat tgagtctggc atggcgcaaa tttggcagcg gcaaagcggt agaagatatt
    26641 gtgaccttgt ttacccagcg caggccggaa atcagcggat ttttagaaat tttcggcaac
    26701 ccacgcagtt aaaaatcaag tttccttgcg cgttggcgcg taaaatagcg cgcaagctga
    26761 tttgaggttc acttttaact atgcaagaga gacacacgga acaggactat cgtgccctgc
    26821 tgattgctga tacgcccatt attgatgttc gcgcccctat agagtttgag caaggcgcaa
    26881 tgcccgccgc tatcaatctg ccgttaatga ataacgatga acgcgccgcc gttggcacct
    26941 gctataaaca gcaaggctca gacgcagcgc tggcgctggg acataaactg gttgcgggtg
    27001 aaattcgtca gcagcgcatg gacgcctggc gggcagcgtg cctgcaaaat ccgcaaggta
    27061 ttctctgctg cgcccgtggc ggtcagcgct cacatattgt gcaaagctgg ttgcatgcag
    27121 cggggattga ttatccgctg gtggcaggcg gttataaggc actgcgccag accgcgattc
    27181 aggcgactat tgaactggca caaaaaccga tagtgctgat tggcggttgt accggcagcg
    27241 gtaaaacgct gttagtgcag caacagccga acggtgttga tctggaaggg ttggcgcgtc
    27301 atcgcggttc ggcgtttggt cgcacgttac aaccacaact tagccaggcg agttttgaaa
    27361 acctgctggc tgccgaaatg ctaaaaaccg acgcccgtca gaatttgcgc ctgtgggtgc
    27421 tggaagacga aagccggatg atcggttcga atcacctgcc ggaatgcctg cgcgagcgaa
    27481 tgactcaggc ggcgattgcg gtggtagaag atccgtttga gatccgtctt gagcgcctga
    27541 acgaagagta tttcttgcgt atgcatcatg attttaccca cgcgtacggc gacgaacagg
    27601 gctggcagga gtattgcgaa tacctgcatc acggactttc ggctattaag cgtcggctgg
    27661 ggctacagcg ctataacgaa ctggctgcaa ggctggatgc agcgctgaca acgcaactca
    27721 ccaccggcag caccgacggt catctggcct ggctggtgcc gttacttgaa gagtattacg
    27781 acccaatgta tcgctatcag cttgagaaaa aagcggaaaa agttgtcttt cgcggtgagt
    27841 gggcggaagt ggcggaatgg gttaaggcgc ggtaatgagg gacgtatttg tattagtcgc
    27901 gacttgatct gacacatggc cttgaaaggt tgagagttac cggttttgat atgagtgtcg
    27961 aatccttatc caaaacaaga ggtaactctc atgcttcata ctgccaatcc cgtcatcaaa
    28021 cacaaagccg gtttgctcaa tctggctgaa gaactcagca acgtatcaaa agcctgtaaa
    28081 atcatgggcg tttcgcgtga tacgttttat cgttaccgcg aactggtcgc tgaaggcggt
    28141 gtggatgcgc agattaatcg tagtcgccgc gctcctaacc ttaagaaccg taccgatgag
    28201 gcaactgaac aggctgttgt tgattacgcc gtcgctttcc cgacacatgg tcagcaccgg
    28261 gccagcaacg agctgcgtaa acagggggtt tttatctccg acagtggtgt ccattctgtc
    28321 tggttactcc ataaccttga gaatctcaaa agacgatact gaacaaattt tatcaggtga
    28381 cgttccgtaa aaagttgtat ggagttctcg atgcagtcgg cgcgactatc agtacccccg
    28441 ctgaaccctg gactatcggg ccgccgtact ggggcatgtc accctggcgt gccgctggtt
    28501 ttccgctaat ggtttatttc ctctttcttt cccggcaaga atttatttta atccggaagg
    28561 attatttatt ttcagatagg gactcacatt aaacacgtaa cattttaatt aatgcatggc
    28621 tgaatatgta ttacacaatg tcagatatcg taaaagacca gtgcaattct atgtaaactc
    28681 tctgactgtt tattttattt gttgtttcag ggtcggaact caccctcaat aaacaactga
    28741 atacgagtag atattcatcg tctgagctat atggctttac acaatagccg acatgtcttt
    28801 tctcagtcag aaattttact tttcctcact acgaagttga cgacttcgat atgggataga
    28861 ctcttaattc aagcaattac tctgcacaag catcaggcaa tccagctcca atatttgtta
    28921 taaaataatg cgtaacatta atttcaccaa caaaaaaaag gtttacacca tccccttgcc
    28981 ctggtatctt accatattta caccacactt tttttgtatc tggatcattc tttgatacga
    29041 ttttcgccac ttgttccctg gc
//
LOCUS       DAPGRN010000059        27979 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPGRN010000059 DAPGRN010000000
VERSION     DAPGRN010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.059"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..719
                     /gene="acrS"
                     /locus_tag="RHW33_002661"
     CDS             57..719
                     /gene="acrS"
                     /locus_tag="RHW33_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV1646148.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFARHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(722..901)
                     /locus_tag="RHW33_002662"
     CDS             complement(722..901)
                     /locus_tag="RHW33_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV1646149.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(985..1869)
                     /gene="yhdJ"
                     /locus_tag="RHW33_002663"
     CDS             complement(985..1869)
                     /gene="yhdJ"
                     /locus_tag="RHW33_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV1646150.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLLEVIAECHRVLKKQGSMYIMNSKENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1955..2251)
                     /gene="fis"
                     /locus_tag="RHW33_002664"
     CDS             complement(1955..2251)
                     /gene="fis"
                     /locus_tag="RHW33_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV1646151.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2277..3242)
                     /gene="dusB"
                     /locus_tag="RHW33_002665"
     CDS             complement(2277..3242)
                     /gene="dusB"
                     /locus_tag="RHW33_002665"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV1646152.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3571..4452)
                     /gene="prmA"
                     /locus_tag="RHW33_002666"
     CDS             complement(3571..4452)
                     /gene="prmA"
                     /locus_tag="RHW33_002666"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV1646153.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4464..5915)
                     /gene="panF"
                     /locus_tag="RHW33_002667"
     CDS             complement(4464..5915)
                     /gene="panF"
                     /locus_tag="RHW33_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV1646154.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5905..6147)
                     /locus_tag="RHW33_002668"
     CDS             complement(5905..6147)
                     /locus_tag="RHW33_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV1646155.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6256..7605)
                     /gene="accC"
                     /locus_tag="RHW33_002669"
     CDS             complement(6256..7605)
                     /gene="accC"
                     /locus_tag="RHW33_002669"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV1646156.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7616..8086)
                     /gene="accB"
                     /locus_tag="RHW33_002670"
                     /gene_synonym="fabE"
     CDS             complement(7616..8086)
                     /gene="accB"
                     /locus_tag="RHW33_002670"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV1646157.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9064..10038)
                     /locus_tag="RHW33_002671"
     CDS             complement(9064..10038)
                     /locus_tag="RHW33_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV1646158.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            10190..12130
                     /gene="csrD"
                     /locus_tag="RHW33_002672"
     CDS             10190..12130
                     /gene="csrD"
                     /locus_tag="RHW33_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV1646159.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPTTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12435..13478
                     /gene="mreB"
                     /locus_tag="RHW33_002673"
     CDS             12435..13478
                     /gene="mreB"
                     /locus_tag="RHW33_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV1646160.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13544..14647
                     /gene="mreC"
                     /locus_tag="RHW33_002674"
     CDS             13544..14647
                     /gene="mreC"
                     /locus_tag="RHW33_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV1646161.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14647..15135
                     /gene="mreD"
                     /locus_tag="RHW33_002675"
     CDS             14647..15135
                     /gene="mreD"
                     /locus_tag="RHW33_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV1646162.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15144..15737
                     /gene="yhdE"
                     /locus_tag="RHW33_002676"
     CDS             15144..15737
                     /gene="yhdE"
                     /locus_tag="RHW33_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV1646163.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKARAGVAQTAKDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15727..17196
                     /gene="rng"
                     /locus_tag="RHW33_002677"
     CDS             15727..17196
                     /gene="rng"
                     /locus_tag="RHW33_002677"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV1646164.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17264..21064
                     /gene="yhdP"
                     /locus_tag="RHW33_002678"
     CDS             17264..21064
                     /gene="yhdP"
                     /locus_tag="RHW33_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV1646165.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGDV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIVLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLNSFDLTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21403..22848
                     /gene="tldD"
                     /locus_tag="RHW33_002679"
     CDS             21403..22848
                     /gene="tldD"
                     /locus_tag="RHW33_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV1646166.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22975..23904)
                     /gene="aaeR"
                     /locus_tag="RHW33_002680"
     CDS             complement(22975..23904)
                     /gene="aaeR"
                     /locus_tag="RHW33_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV1646167.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24087..24290
                     /locus_tag="RHW33_002681"
     CDS             24087..24290
                     /locus_tag="RHW33_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV1646168.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24298..25230
                     /gene="aaeA"
                     /locus_tag="RHW33_002682"
     CDS             24298..25230
                     /gene="aaeA"
                     /locus_tag="RHW33_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV1646169.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25236..27203
                     /gene="aaeB"
                     /locus_tag="RHW33_002683"
     CDS             25236..27203
                     /gene="aaeB"
                     /locus_tag="RHW33_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV1646170.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLCIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27295..27567
                     /locus_tag="RHW33_002684"
     CDS             27295..27567
                     /locus_tag="RHW33_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV1646171.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27623..27886)
                     /gene="yhcN"
                     /locus_tag="RHW33_002685"
     CDS             complement(27623..27886)
                     /gene="yhcN"
                     /locus_tag="RHW33_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV1646172.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6706 a         7016 c         7448 g         6809 t
ORIGIN      
        1 accttcgcta ttttctcact ctgtgtcgaa tatatttatt acctgaataa ttaatcatgg
       61 caaaaagaac caaagccgaa gctctgaaga cccggcaaga actgattgaa actgccatcg
      121 cccagtttgc gcggcatggc gtaagcaaga cgacgctcaa cgacattgcc gacgccgcta
      181 acgttacgcg tggcgctatc tactggcact tcgaaaacaa gactcaactg tttaatgaga
      241 tgtggttgca acagccttca ttgcgggagt taatccagga acacttgacg gctggattag
      301 agcatgaccc gtttcaacaa ttgcgtgaaa aattgattgt cggcttgcaa tatattgcca
      361 aaattccccg ccagcaggcg ttgctgaaaa tcttatatca caaatgtgaa tttaatgatg
      421 agatgctggc cgagggagtg atacgcgaaa agatgggctt taatccgcag actctccgcg
      481 aagtattgca ggcgtgtcag caacaaggtt gtgtagcaaa taacctcgat ttagatgttg
      541 tgatgattat tattgatggt gccttcagcg gaattgttca aaactggtta atgaatatgg
      601 cgggttatga tctttataaa caagcccccg ctctggtcga taacgtatta agaatgttca
      661 tgccagatga aaacataacg aaattaattc atcaaacgaa tgaattaagt gtcatgtaaa
      721 ttcacattct gtcctgaaaa ttcagttcat cacagttaac cagttttagt ggattaactg
      781 agttcatatt gcggcatttg tctgtttctg ttgccagcag ttcaatccac tgcatcagta
      841 aagtatcaaa cagaaaaacg aagacagcga aaacgacgag ccaccaatac ttgcgaatca
      901 ttacctgagc ctgtgcatgt ttaaccaagt ctggcaggaa atatacacga aatgaataat
      961 ctgaaaacca ggcttacact tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa
     1021 ccgacttcgt tttgacaggt tgccggtctt tctttttttc actttcgcca gttcttccgc
     1081 agagtaatgc gacgcgacat ccagccgtcg aagccccatt ttgatgtact cgctgttgat
     1141 ctcaatacca atgaattttc gcccgctgga gacggctacg gcaccggtag taaagctacc
     1201 ggcaaacggg tcgagaacga tatcgcctgg gttggaagag gcgagaataa tgcgtttcag
     1261 taaggcttcc ggtttttgcg tcgggtggtt ctcatattca tccatcaaat aacgcacgcg
     1321 cggaaaatcc cagacgttac ccggtacttt ttgatgattg taaggctgtg gaggattttt
     1381 gcgataatcg atcaacgcgc gctgcgatcc ggttttggct tctaccagaa tagcatcacc
     1441 gttgaatgtg tagttctttg cgtctttcac catcatcagg atgggttcgt acatggagcc
     1501 gtagtgtttt ttcgcctgca ctccagaact gtcatatgac cagacgatgc gacttttgat
     1561 ggtaaaaagc ttgcggcact ggagatcgat aaagggcatg ttttccttac tgttcatgat
     1621 gtacatgctg ccctgctttt tcagaacgcg gtggcactct gcaatcactt ctaacagcca
     1681 gtcgataaac agatcttctt tccaggcttc gatcagacca tcaaaatttt taccgatgtt
     1741 atacggtggg tcggcaaaga tcagatcgac gctttcagta ggtagctttt taagttcggc
     1801 aagggcatca ccgtgaataa tggtcttagc ttcattacca aaccgggtcg gttcacatcc
     1861 tgttctcatg gtcactccct ttgtgacacc tataaaaaag gcgcttcccc atgccgagta
     1921 gcgccttttt aatcaagcat ttagctaacc tgaattagtt catgccgtat tttttcaatt
     1981 ttttacgcag cgtaccacgg ttgatgccca tcatcagcgc agcacgggtc tggttaccac
     2041 gggtgtattg catcaccatg tccaacaggg gctgttctac ttcagccagt accagctcat
     2101 agaggtcatt cacatcctga ccattcagtt gagcaaaata gttcttcagt gcctgtttaa
     2161 ccgagtcacg caggggtttt tgggttacct gatcctgaga gttaacggta gaaacggtca
     2221 gtacgtcaga atttacgcgt tgttcgaaca tagttctgtc agctctttat ttctgtttac
     2281 gcaaaatttt cgaagtatgc ctccaacgcc tccagctgtt cgctggcatc ctcaatggcg
     2341 ttgaatgtgc gccgaaactg gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt
     2401 ttacgtgcaa ttcggtaccc ttttgccgga ccataaaagt catgcagttc ccgaacgtgc
     2461 gcgcaaagca agcgcttaac ctctgccaaa ggcagcgggg gcagcaactc cccagtgtcc
     2521 agataatgct ggatttcccg aaagatccag ggtcttccct gagctgcgcg gcctatcatc
     2581 agggcatccg cccctgtata gtcgagcaca gctctggctt taagcgggtc agtaatgtcg
     2641 ccattcgcga taaccggaat ggaaactttc tgcttaactg cccgaatact gtcgtactca
     2701 gcttctccat tgaacaaaca ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca
     2761 cagtcttcag ccagttgggc aatctcttcg cagttacggt gttccggtgc ccagccggtg
     2821 cgaatcttca gggtaacagg aacgtccact gcattgacga cctcggtaag gatcgattta
     2881 acgacatccg ggtactgcaa gagggctgaa cctgcgagct tgcgattcac ttttttagcc
     2941 gggcaaccca tattgatatc aataatctgg gcaccgcttt ccacgttaat acgtgctgca
     3001 tctgccattt ctttcggatc gctaccagca atttgcacgg tgcgaatacc gggttcatca
     3061 atgtgcacca tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc
     3121 atctcggata ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct
     3181 gtaatgccag ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc
     3241 atgagttaag aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca
     3301 cgagatgaaa ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa
     3361 atgagcgtag cgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat
     3421 ttacactcaa agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca
     3481 cgcaaaattg cgtaaataat cagcaaattt acgtgccttt tgtgagcttg ctcgcacttc
     3541 gccctgcgtc accctacggc gatgcgaagg ttaattctta cgaccggtaa tgcggcacca
     3601 ctcttctttt tccacgaccg ggtccagtgc gaagctgtcg gcataagctt cgcaaacgct
     3661 ctctgcctgg cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa ccggcaggac
     3721 gctgattaac ggtgccagtt cacgtaacgg gcctgcaagg atgttagcga ccaccacgtc
     3781 ggctttcatt tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc
     3841 attacgttcg gcgttatcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat
     3901 ggcttttgct gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa
     3961 gtcgatgact gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt
     4021 ggttggatgg gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt
     4081 ttcgtccggc acatcacgcc agctagggca gatccacagt cgttcaccaa agcgcatcgg
     4141 gtggaaatta tccatccatt cgcgctccca gtctttatct tctagttgtt cgattttatg
     4201 cgcgaagcct gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc
     4261 ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg
     4321 cagcggctca aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc
     4381 ttccatcagc gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg
     4441 gatccaaggc atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg
     4501 gatgtaccga aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg
     4561 atagggtgga agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg
     4621 ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc
     4681 accagcggcc acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg
     4741 atcatttctg gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt
     4801 accgccgaca tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga
     4861 tagagatctt taatgatcgt agcggaactt tgtagcaatt gggcgttaat tgtcgacatg
     4921 atcgcagcca taggtgcagc cagaaagatc ccggcagcaa acggtggcag cacttttacc
     4981 attaacgtcg ggatcaccag atccggtacg gtgagatcgg ggatcactgc acgacctaat
     5041 gctccggcca ggtgcatacc gaacatcagg attgcgacta caatcgtacc gatgatgatc
     5101 ccccggtgta ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg
     5161 cctataacgc caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata
     5221 tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg
     5281 ttgcttaagc cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc
     5341 atcacaagcc cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta
     5401 tataacgcaa tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg
     5461 gtttccagca ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc
     5521 agcaaactca aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata
     5581 tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata
     5641 ccgagtgaaa gccagactgc cggaagctga atcatcgcca gcaataccca gccaaggccg
     5701 tatttgtaag cagctccagg cccgccgata aacgaactgg cactgatata agtcgcggtg
     5761 agcgtcatcg ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg
     5821 aaggtgccgg tgctccgttt acgcatcgca taaacagaga taccgaacac caccaccaga
     5881 taggcgacca gcggtagaat tacttcaagc tgcatcgtca tcctccagtg ggatatcgcg
     5941 atagataaat ttcaccatcg cccagcacag tccaataaac agcagcggcg tcaggatgca
     6001 ggccatctca aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc
     6061 ggctactaac caaactgcca gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt
     6121 atgggcctga acaaaacgag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat
     6181 tgtaccttat gggggttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt
     6241 gacgcttttg cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt
     6301 agtgccacca tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt
     6361 tttgataccg tcgatgatca gctcctgcag cgcatttttc atgcgagcaa tcgccacgtc
     6421 acggttttca ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt
     6481 gtagcccgcg tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg
     6541 ggtgattttg cccgggcttg gcaggaaggt gttcggatct tcggcgttga tacggcattc
     6601 caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc
     6661 gatacgcagc tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc
     6721 tacctgaata cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc
     6781 gaaagtacct gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat
     6841 gtagcgacgc agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg
     6901 gcggcgctgc atggagcagt cacgttccgc cagatagata gcgttgccct gaccgtcagc
     6961 cagtacctga atctcgacgt ggcgaggatt ttccaggtat ttctccatgt aaaccatatc
     7021 gttgctgaaa gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc
     7081 gtcgccgcgc actacgcgca taccgcgacc accgccgccg ccggaggctt tgataatcac
     7141 cggataacca atgcgtttag caatggcacg gtttttatcc atatcgtcgc ccagcgggcc
     7201 gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc gccgcgattg cggatacttt
     7261 gtcgcccatc aggcgaatgg tttctgcttt cgggccaatg aagataaagc cggagcgttc
     7321 aacctgctcg gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac
     7381 tgcgccggtg atttcagcgg cgctgatgat tgccgggatg ttcagataac tttttactga
     7441 cggagcaggg ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc
     7501 cgcgctggag tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg
     7561 caatgcaatc tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact
     7621 cgatgacgac cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt
     7681 tcacggtacc ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc
     7741 acagggtatc gcccacgttg actttctgac ccacttcgat gaacgctttt gcgtccgggc
     7801 ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg
     7861 ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg
     7921 gctgctgcat cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag
     7981 ctgcacggct aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg
     8041 attcttcaac cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta
     8101 ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg
     8161 acagctgtat gcatagcgat aaattccagc aggccgggac gccggtctat tttgccccag
     8221 agtgtcaatg ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa
     8281 aacaaaatat accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag
     8341 ggaggacgag gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt
     8401 atcagggaag ataatcaaca gctaacatgt aaataacctt caacaccgtg taatttgcaa
     8461 caagccgcac aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt
     8521 gaaaagctgt aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcttcacaga
     8581 ccacaaataa tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg
     8641 ctggggatta cccaattttc actgcatcgt ctggatagat gtgaatgcta aagcacgcaa
     8701 aattaaccga ttgacaatgc tcagcattaa ataggttcga gtgattaatt tgtagtcaaa
     8761 caaaatcaga ttattcaccc catggtacca gaaatcagta acaggttaag cgtagcgtcg
     8821 cccaggcaaa gcaccataaa cctaacaggc gacggaggcg tatcaataat agctgtttag
     8881 tggagtatca atgcgcatca atttaaggtc agtcgaaatc atgactttcc agtttggatg
     8941 atgttgtaca acttcatgac caaggtgttc gacgaaagtt gtccggctaa ccgcttttcc
     9001 cgttaaccga ttgaataatg atttcaaaat gagttccctc tcttattaat ccttgctaaa
     9061 tggttagtta accttcacca gcgtgcgacc ctggatctgg ttattaatga tggcctcggc
     9121 aaacttcggt gcctctgcca gagatatctc ttttgccgcc tgggtataga atgattccgg
     9181 taaatcggcg accagtcgct gccaggcttg tgcgcggcgt tctggtggcg tcattactga
     9241 atccacccct tgcaaacgga cattacgcag aataaatggc atgaccgtgg ttggcagagt
     9301 aaaaccaccc gccagaccac aggccgccac gcagccgccg taattcattt gcgccagcac
     9361 ttttgccagc actttgtcgc caacagtgtc aattgcccca gcccagactt gtttttccag
     9421 aggacgggat tcggcaaact catcacgagg gagaatacgg ctggcaccta aacttttcag
     9481 atattcatgg gtactttcgc gaccggaaac ggcaacgacc tgataaccca acttatgcag
     9541 cagcgccacg gcggtactgc cgacgccacc actggcaccc gtcacgacaa tctccccgtc
     9601 ctgcgggcga acaccggcat cttccagcgc catcacacac agcatggcgg taaaaccggc
     9661 agtaccgata atcattgctt tacgcgcgtc cagcccttgc ggcatggcaa ccagccagtc
     9721 acctttcact cgcgcctgct ccgccagccc accccagtgg ttttcaccaa cgccccagcc
     9781 agtgagtaac acctcctgac cggcatgaaa acgcggatct tcgctggtgc gtacagttcc
     9841 ggcaaaatcg atcccaggaa tcatcggaaa attacggatg atttttccct taccggtaat
     9901 cgccagcgca tctttatagt tcaggctcga ccagtgaaca tcgaccgtga catcgccctc
     9961 cggcaggcga ctttcgtcca gagtctgtac tgatgcgaga gttttgccgt cctgctgttc
    10021 taaaagtaac gcctgcataa gtggtcctca tgtgcatgat ggattggaaa ataatttctg
    10081 aagactatac tcgctaatgg aaaagcaaag ccgatgaagc gcaataaatt gcgagataaa
    10141 tctgatttgc tagtatgccc gcttcctcac tatcggagtt aacacaagga tgagattaac
    10201 gacgaaattt tcggcctttg ttacgctgct caccgggtta acaatttttg tgactttgct
    10261 gggctgttcg ctaagtttct acaacgccat tcagtataag tttagtcatc gcgttcaggc
    10321 ggtggcgacg gcgattgata cccaccttgt gtcgaatgac ttcagcgcat taaggccaca
    10381 aattaccgaa ttaatggtgt cagcagatat cgttcgtgta gacctgctgc atggtgataa
    10441 acaggtttat accctggcca gaaatggtag ttatcgtccg gttggcacca gcgatctgtt
    10501 tcgtgaactg agcgttccgt tgataaagca tccggggatg tcgttgcgtc tggtttatca
    10561 ggatccgatg ggcaactatt tccattcgtt gatgaccacc gcgccgctca cgggggcgat
    10621 tggctttatc attcttatgc tctttctggc ggtacgctgg ttacaacggc aacttgccgg
    10681 gcaagaattg ctggaaaccc gggctactcg tatcttaaac ggtgagcgtg gctctaatgt
    10741 gttgggaacc atctatgaat ggccgcccac aaccagcagt gcgctggata cgctgcttcg
    10801 tgaaattcag aacgcacgcg aacaacacag ccgtcttgat acgctgatcc gctcttatgc
    10861 cgcacaggac gtgaaaaccg gcctcaataa ccgactcttt ttcgataatc agttagcaac
    10921 gttactggaa gatcaggaga aagtaggtac ccacgggatc gtgatgatga ttcgtctgcc
    10981 ggatttcaat atgttgagcg atacctgggg gcacagccag gttgaagaac agttcttcac
    11041 tctgacgaat ctgctgtcga catttatgat gcgctaccct ggcgcactgc tggcgcgtta
    11101 ccaccgcagt gattttgctg cgctgttacc gcaccggacg ttaaaagagg cagagagcat
    11161 cgccggtcag ttaatcaaag ccgtcgatac cttgccgaac aataaaatgc tcgatcgcga
    11221 cgatatgatc cacattggta tctgcgcctg gcgtagtggt caggataccg agcaggtaat
    11281 ggaacatgca gagtctgcca cgcgtaatgc gggattgcag ggcggcaata gctgggctat
    11341 ttacgatgac tcgttgcctg aaaaaggacg cggtaatgtt cgctggcgta cgcttatcga
    11401 gcaaatgctc agtcgcggcg gcccgcgcct ttatcaaaaa ccggcggtta ctcgcgaagg
    11461 tcaggttcat catcgcgaac tcatgtgccg catctttgat ggtaatgaag aggttagctc
    11521 ggcggagtat atgccgatgg tcttgcagtt tggcttatcg gaagagtatg accgtctgca
    11581 aatcagccgt cttattccac tattgcgtta ctggccagag gaaaatctgg cgattcaggt
    11641 taccgttgag tcgctgattc gcccgcgttt tcagcgttgg ctgcgcgata cgttaatgca
    11701 atgtgaaaaa tcacaacgaa aacgcataat tattgaactt gcagaggccg atgtaggtca
    11761 acatatcagt cgtttacaac ctgttattcg tttagtgaat gctttagggg tacgggtagc
    11821 cgtcaaccag gctggtttga cgctggtaag caccagttgg atcaaagaac ttaatgttga
    11881 gttactcaag ctccatccgg ggctggtcag aaacattgag aagcgaacgg agaaccagct
    11941 gctggttcaa agcctggtgg aagcctgctc agggaccagc acccaggttt acgccaccgg
    12001 tgtgcgttcg cgaagcgagt ggcagaccct gattcagcgc ggtgttacag gcgggcaagg
    12061 ggattttttc gcgtcctcac agccacttga tactaacgtg aaaaaatatt cacaaagata
    12121 ctcggtttaa cctgccgttt aatccgtttt cacgtagaat aacgcgcgct gcgtctcatg
    12181 ggagtgtgct tgtctgctcg ccagattgtt gcagcacata tgcagatgaa tgaccttacg
    12241 cggttgcaaa caggcgagga atgctgctga tgcattaagc ctttctggac tcaggcagag
    12301 atttgtaaca aaggaaacga actgcactaa ttttcaccgt agcagatgat ttttgcgcct
    12361 tgtcgctgct gcgtgtggtt ggtaaagtaa gcggattttc ttttccgccc cagctttcag
    12421 gattatccct tagtatgttg aaaaaatttc gtggcatgtt ttccaatgac ttgtccattg
    12481 acctgggtac tgcgaatacc ctcatttatg taaaaggaca aggcatcgta ttgaatgagc
    12541 cttccgtggt ggccattcgt caggatcgtg ccggttcacc gaaaagcgta gctgcagtag
    12601 gtcatgacgc gaagcagatg ctgggccgta cgccgggcaa tattgctgcc attcgcccaa
    12661 tgaaagacgg cgttatcgcc gacttcttcg tgactgaaaa aatgctccag cacttcatca
    12721 aacaagtgca cagcaacagc tttatgcgtc caagcccgcg cgttctggtt tgtgtaccgg
    12781 ttggcgcgac ccaggttgaa cgccgcgcaa ttcgtgaatc cgcgcagggc gctggtgccc
    12841 gtgaagtctt cctgattgaa gaaccgatgg ctgccgcaat tggtgctggc ctgccggttt
    12901 ctgaagcgac cggttctatg gtggttgata tcggtggtgg taccactgaa gttgctgtta
    12961 tctccttgaa cggcgtggtt tactcctctt ccgtacgcat tggtggtgac cgtttcgacg
    13021 aagctatcat caactatgtg cgtcgtaatt acggttctct gatcggtgaa gccaccgcag
    13081 aacgtatcaa gcacgaaatc ggttcggctt atccgggcga tgaagtccgt gaaatcgaag
    13141 ttcgtggccg taacctggca gaaggtgttc cacgcggttt taccctgaac tccaacgaaa
    13201 tcctcgaagc actgcaggaa ccgctgaccg gtattgtgag cgcggtaatg gttgcactgg
    13261 aacagtgccc gccggaactg gcttccgaca tctccgagcg cggcatggta ctcaccggtg
    13321 gtggcgcact gctgcgtaac cttgaccgtt tgttaatgga agaaaccggc attccagtcg
    13381 ttgttgctga agacccgctg acctgtgtgg cgcgcggtgg cggcaaagcg ctggaaatga
    13441 tcgacatgca cggcggcgac ctgttcagcg aagagtaatc ggatgcaggc aggggaagcg
    13501 tctgtttacc ctgcctggtc tgatacgaga atacgcataa cttatgaagc caatttttag
    13561 ccgtggcccg tcgctacaga ttcgccttat tctggcggtg ctggtggcgc tcggcattat
    13621 tattgccgac agccgcctgg ggacgttcag tcaaatccgt acttatatgg ataccgccgt
    13681 cagtcctttc tactttgttt ccaatgctcc tcgtgaattg ctggatggcg tatcgcagac
    13741 gctggcctcg cgtgaccaac tagaacttga aaaccgggcg ttacgtcagg aactgttgct
    13801 gaaaaacagt gaactgctga tgcttggaca atacaaacag gagaacgcgc gtctgcgcga
    13861 gctgctgggt tccccgctgc gtcaggatga gcagaaaatg gtgactcagg ttatctccac
    13921 ggttaacgat ccttatagcg atcaagttgt tatcgataaa ggtagcgtta atggcgttta
    13981 tgaaggccag ccggtcatca gcgacaaagg tgttgttggt caggtggtgg ccgtcgctaa
    14041 actgaccagt cgcgtgctgc tgatttgtga tgcgacccac gcgctgccaa tccaggtgct
    14101 gcgcaacgat atccgcgtaa ttgcagccgg taacggttgt acggatgatt tgcagcttga
    14161 gcatctgccg gcgaatacgg atattcgtgt tggtgatgtg ctggtgactt ccggtctggg
    14221 cggtcgtttc ccggaaggct atccggtcgc ggttgtctct tccgtaaaac tcgataccca
    14281 gcgcgcttat actgtgattc aggcgcgtcc gactgcaggg ctgcaacgtt tgcgttatct
    14341 gctgctgctg tggggggcag atcgtaacgg cgctaacccg atgacgccgg aagaggtgca
    14401 tcgtgttgct aatgaacgtc tgatgcagat gatgccgcag gtattgcctt cgccagacgc
    14461 gatggggcca aagttacctg aaccggcaac ggggatcgct cagccgactc cgcagcaacc
    14521 cacgacagga aatgcagcta ctgcgcctgc tgcgccgaca cagcccgctg ctaatcgctc
    14581 tccacaaagg gctacgccac cgcaaagtgg tgctcaaccg cctgcgcgtg cgccgggagg
    14641 gcaatagtgg cgagctatcg tagccaggga cgctgggtaa tctggctttc tttcctcatt
    14701 gcgctgttgc tgcaaatcat gccctggccg gataacctga ttgttttccg gccaaactgg
    14761 gtgttactca tcttgttgta ttggatcctg gccttgcccc atcgcgtaaa tgtgggcaca
    14821 ggttttgtga tgggtgccat actggatctg atcagcggct cgacgcttgg cgtacgcgta
    14881 ttggcgatga gcatcattgc ttacctggtg gcgctgaaat accagctttt ccgcaacctc
    14941 gcattatggc agcaggcgct ggtcgtcatg ttgctttcgc tggtggtgga tattattgtt
    15001 ttctgggcag agtttttagt gattaacgtc tctttcagac cggaagtgtt ctggagtagt
    15061 gtagtcaatg gggtgctctg gccgtggatt ttcttgctga tgcgtaaagt ccgtcagcag
    15121 tttgcagtgc aataaaggtt tctatgactt ctctgtattt agcttccggt tctccgcgtc
    15181 gtcaggagtt acttgcgcaa cttggcgtga cctttgaacg tattgttacg ggcattgagg
    15241 agcagcgtca gccgcaggag agcgcgcagc agtatgttgt gcgtctggcg cgcgagaaag
    15301 cacgggcagg tgtcgcgcaa acggcgaagg atctcccggt gctgggtgcg gatactatcg
    15361 ttatcctgaa cggagaagtg ctggagaaac cgcgcgacgc agagcatgcg gcgcagatgt
    15421 tgcgcaaatt atcgggtcag acccatcagg tgatgacggc agtggcgttg gccgacagcc
    15481 agcacattct cgattgcctg gtggtcaccg atgtgacttt cagaacgtta acagacgaag
    15541 acatcgcggg ctatgtcgcc agcggtgaac cgttagataa agcaggtgca tacggtattc
    15601 aggggctggg tggctgtttt gtcaggaaga taaatggcag ctatcacgcc gtagtcggct
    15661 taccgctggt tgaaacgtat gaattattaa gtaattttaa cgcactgcgt gagaaaaggg
    15721 ataaacatga cggctgaatt gttagtaaac gtaacgcctt cggaaacgcg agtggcgtat
    15781 attgatggcg gtattctgca ggaaattcat attgaacgtg aggcgcgacg cggaatagta
    15841 ggcaatatct acaagggtcg tgtaagtcgt gtacttccgg gtatgcaggc ggcttttgta
    15901 gatattgggc tggataaagc cgcgtttctt catgcatctg acatcatgcc gcacaccgaa
    15961 tgtgtggcgg gtgaagaaca aaagcaattc acggtgcgcg acatctcgga actggttcgt
    16021 caggggcaag atctgatggt gcaggtggtg aaagatccgc ttggcactaa gggggcgcgc
    16081 ctgaccaccg atatcacgct gccttctcgc tatctggtct ttatgccagg ggcttctcac
    16141 gttggggttt cccaacgaat tgaaagcgaa tcagaacgtg aacgcctgaa aaaagtggtc
    16201 gcagagtatt gcgacgagca gggcgggttt atcatccgta ccgcagcgga aggggttggc
    16261 gaggctgaac tggcctccga tgccgcttat ctgaaacgcg tctggaccaa agttatggag
    16321 cgtaaaaagc gcccgcagac ccgttatcag ctgtacggcg aactggcgtt ggcgcagcgt
    16381 gttctgcgtg atttcgccga tgccgaactg gaccgcattc gcgttgactc acgcctgact
    16441 tacgaagcgt tacttgagtt cacctcggag tacattcccg agatgaccag caagctggag
    16501 cattacaccg gacgccagcc gattttcgat ctctttgatg tcgaaaacga aatccagcga
    16561 gcgctggaac gcaaagtaga actgaaatcc ggtggttatc tgattatcga ccagaccgaa
    16621 gcgatgacca ccgtggacat caataccgga gcgtttgtcg gtcatcgcaa tcttgacgac
    16681 accattttca ataccaatat tgaagcgacg caggctatcg ctcgccagtt acggttgcgc
    16741 aatctgggcg ggattatcat tattgatttc atcgatatga ataatgaaga tcaccgccgc
    16801 cgagtgctgc actcgctgga gcaggcgttg agcaaagacc gggtgaaaac cagcgttaat
    16861 ggtttttcgg cgctggggct ggtggagatg acgcgtaaac gcacccgcga aagcattgag
    16921 cacgtactgt gtaacgaatg cccaacctgc cacggtcgcg gaacggtgaa aaccgtggaa
    16981 acggtatgct atgaaatcat gcgcgagatt gttcgtgtcc accatgctta cgactccgac
    17041 cgtttcctgg tctatgcttc tccggcagta gctgaagcct tgaaaggcga agagtcacac
    17101 tcgctggcgg aagtggaaat tttcgttggt aaacaggtta aagtacaaat tgaaccgctc
    17161 tataaccagg agcagtttga cgtcgtaatg atgtaaacag atgctgggcc gccatccggc
    17221 aaagggtttt tgagtcacat atttagcaga caaggagtga caggtgaggc gattgccggg
    17281 gattttactg cttactggag ccgcgctcgt tgtgatcgct gccctgctgg ttagcggcct
    17341 gcgtattgct ttaccgcatc ttgacgcctg gcgtccggaa atcctcaaca aaatagaatc
    17401 cgcgactggc atgccggttg aagccagtca gctctcagcc agctggcaga attttggccc
    17461 gacgcttgaa gcacacgaca tccgtgcaga actaaaagat ggcggcgaat tttcggttaa
    17521 acgcgttact ctggcgctgg atgtctggca gagcctgtta catatgcgct ggcagtttcg
    17581 cgacctcact ttctggcagc tgcgctttcg caccaacact cctatcacca gcggtggtag
    17641 cgatgacagt ctggaagcca gtcacatcag cgatctgttt cttcgtcaat ttgaccattt
    17701 cgatctccgc gacagtgaag tcagtttcct gacgccatcc ggtcagcgcg ccgagctggc
    17761 gatcccacaa ctcacctggc tgaacgatcc acgtcgacac cgtgcggaag gcctggtaag
    17821 cctctccagc cttaccggac agcacggcgt gatgcaggtg cgcatggatt tgcgcgatga
    17881 tgaggggttg ttaagcaatg gtcgcgtctg gctccaggcg gatgacatcg acctgaagcc
    17941 gtggctcggt aaatggatgc aggacaatat tgccctggaa acggcacagt tctcccttga
    18001 aggctggatg acgatcgaca aaggcgatgt aaccggcggt gacgtctggc tgaaacaggg
    18061 cggtgccagc tggttgggcg agaaggaaac gcatacgctg tcggtggata atctgaccgc
    18121 gcatattacg cgtgaaaatc cgggctggca gttctctatt cccgatacac ggatcacgat
    18181 ggacggcaaa ccctggccga gcggagcatt gacgctggcc tggataccgg aacaggacgt
    18241 tggcggcaaa gacaataaac gcagtgacga actccggatt cgcgccagta atctggagct
    18301 ggcaggcctg gagggcatac gcccgctggc cgcgaaactt tcacctgcac tgggtgatgt
    18361 ttggcgctcc acacaaccga gcggcaagat taacactctg gcgctggata tcccgcttca
    18421 ggcggcagac aagacccgtt ttcaggcatc gtggagcgat ctggcctgga agcaatggaa
    18481 attattaccg ggtgcggaac acttctccgg gacgctttcc ggcagcgtag aaaatggttt
    18541 gcttaccgcg tcgatgaagc aggcaaagat gccttacgaa acggtattcc gtgcgccact
    18601 ggaaatcgcc gacggccagg caactataag ctggctgaac aatgacaaag gtttccagct
    18661 ggatgggcgt aatattgacg ttaaagccaa agccgtccat gcgcgcggcg gttttcgtta
    18721 cctgcaacct gctaacgatg aaccctggct gggtattctg gctggcatca gtaccgatga
    18781 tggttcacaa gcctggcgct atttcccgga aaacttgatg ggtaaagacc tggttgatta
    18841 cttaagtggc gcgattcagg gcggtgaagc ggataacgcg acgctggttt atggtggcaa
    18901 tccgcaactc ttcccctata aacacaacga aggtcagttt gaagtgctgg tgccgctgcg
    18961 caacgcgaag tttgccttcc agccggactg gcctgcatta actaaccttg atattgaact
    19021 ggactttatt aacgacggtt tatggatgaa aaccgatggc gttaatctgg gcggcgtgcg
    19081 cgcgagtaat ctcaccgcag tgatccctga ctactcgaaa gaaaaactgc tgattgacgc
    19141 tgacattaaa ggtccgggta aagccgttgg cccttacttt gatgagacac cgctgaaaga
    19201 ttctctgggg gcgaccctgc aagaactcca gctcgacggc gatgtgaatg ctcgcttaca
    19261 tcttgatatc ccgctgaacg gcgaactggt aaccgcgaaa ggtgatgtga cgctgcgtaa
    19321 taacagtctg tttatcaaac cactcgacag caccctgaaa aatttgagcg gtaaattcag
    19381 ctttatcaat ggcgatctgc aaagtgaacc actgacagca agctggttta atcagccgtt
    19441 gaacgtggat ttttccacca aagaaggggc aaaagcctac caggtcgcgg tgaatctcaa
    19501 cggtaactgg caaccagcga aaaccggcgt tctgcctgca gcggtgaacg aagcattgag
    19561 tggcagcgtg gcgtgggatg gtaaagtggg cattgttctg ccttatcatg ctggcgcgac
    19621 gtataacgta gagctaaacg gcgatttgaa gaatgtgagc agtcacttac cttcaccgtt
    19681 agccaaacct gcgggtgaac cactgccggt aaacgttaag gttgatggca atctcaacag
    19741 ctttgattta accggacagg ctggtgcgga taaccatttc aatagccgct ggttgctcgg
    19801 tcaaaagctg acgctcgacc gtgctatttg ggcggcagac agtaaaacgc tcccgccgtt
    19861 gccggaacaa agtggcgttg aactcaatat gccgccgatg aatggtgccg agtggctggc
    19921 cctgttccag aaaggcgcgg cggagagtgt cggtggtgca gcgagtttcc cacaacacat
    19981 aacgttacgt acgcctatgt tgtcgctggg aaatcagcaa tggaataacc tgagtattgt
    20041 ttcgcaaccg acggcaaatg gcacccaggt tgaggcgcaa gggcgtgaaa tcaacgccac
    20101 gctggcgatg cgtaataacg cgccgtggct ggcgaatatc aaatatcttt attacaaccc
    20161 gagcgtggcg aaaactcgtg gtgattcaac gccgtcatca cctttcccga caacggagcg
    20221 cattaacttc cgtggctggc cggacgcaca aatacgatgc gcagagtgct ggttctgggg
    20281 gcaaaaattc ggtcgtattg acagtgatct caccatttct ggcgatacat taacgctgac
    20341 caatggactg attgataccg gtttctcgcg gcttactgcc gatggtgaat gggttaataa
    20401 tccggggaat gaacgtacct cgctgaaagg aaaactgcgc gggcagaaaa ttgatgccgc
    20461 cgcagaattt tttggtgtca cgacgcccat acggcagtcg tcatttaatg tggattacga
    20521 tttacactgg cgtaaagcac cgtggcagcc agatgaggcg acgttgaatg gcatcattca
    20581 tactcaactg ggtaaaggcg aaattaccga aatcaatacc ggacatgccg ggcaattgct
    20641 gcgcttattg agcgtagatg ccctgatgcg taagctgcgt tttgatttca gagacacttt
    20701 tggcgaaggg ttctattttg actccattcg cagcaccgcg tggattaaag acggcgttat
    20761 gcacaccgac gacacgctgg tggatggcct ggaggcggat atcgccatga aagggtcggt
    20821 aaatctggta cgtcgcgacc tgaatatgga agcggttgtc gcaccagaga tttctgcgac
    20881 ggtgggcgtg gctgcggctt ttgcggttaa ccccattgtt ggcgcggcag tgtttgccgc
    20941 cagtaaagtg ctggggccgc tgtggagcaa agtctccatt ttgcgctatc acatttcggg
    21001 tccgctggac gatccgcaaa tcaacgaagt gttgcgccaa ccgcgtaaag aaaaagcgca
    21061 atgatttgac gagggcgcgt aattgcccca atctcataag ataatcgttg ccaaaggcca
    21121 acgagccaga acataaccgt aggtcggata gggcgttcac gccgcatccg gcagccgttg
    21181 cctgatgcga cgctgtgcgt cttatcaggc ctacaaacgg aacataaccg taggtcggat
    21241 aaggcgttta cgccgcatcc gacagccgtt gcctgatgcg acgctgtgcg tcttatcagg
    21301 cctacaaacc ggaacataat cgtaggccgg ataaggcgtt tacgccgcat ccggcagccg
    21361 taaaaaatcc tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta
    21421 agtgaacaat tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt
    21481 caactggccg aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa
    21541 tcctgggttt tagaagaccg tattattaaa gatggttctt acaacatcga tcagggcgtt
    21601 ggcgtgcgtg caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg
    21661 ctggcgctgg aacagagtgc acaagcggcg cgcaccatcg tccgtgatag tggtgatggc
    21721 aaagtacaga cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg
    21781 caaagcatga gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgcccgc
    21841 gaagcggaca agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt
    21901 ctggttgcgg ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc
    21961 gtgagcgttc tcgtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt
    22021 cgttttggtt atgagttctt ccttgccgat ctcgacggcg aagtccgtgc ggatgcatgg
    22081 gcaaaagaag cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc
    22141 accatgccgg tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt
    22201 cacggtctgg aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg
    22261 gagctggtgg cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt
    22321 ggttcggtgg cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac
    22381 ggcattctga aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg
    22441 actggcaacg gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc
    22501 tatatgctgc cgggtaaatc gaccccgcag gaaattattg aatccgttga gtacggtatc
    22561 tatgcaccga actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc
    22621 acttcagaag catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg
    22681 ttgattggtt ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa
    22741 ctggataacg gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggtgtgggc
    22801 cagccaacgt tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcatttt
    22861 tttagaagga taattaatct ttctacgtgc acgaacggtc ccctcgctcc tctggggtta
    22921 gggtgagggg aacccgttag cacaggtttg tacaacgtaa cagtacaata tgaattactt
    22981 ctctttcccg cgcccgtgca tctcctgaaa caatttaccg acctcaacaa aataatccgt
    23041 cagcgagttg atcacgacct gtaccttcag cggcagctta tctttttcgg tatataacgc
    23101 ataaaccggg cgtggatctg actgataacg cggcagcagg atctccagct ccccacgatt
    23161 gatctcgttg atcacccaca tcagcggcac gtaggcgatc ccggcacccg ccgttagcca
    23221 gcgcaccagc gtcatcggat cattagtcac aaatctccct tgcgggatca ggcgagtcga
    23281 gatcccttcc ggtgcaatca gttcaaattc attgtcgggc cgcacgctgt attcaagcca
    23341 tgaatggcta cttaaatcgg cgggtttttc cggtatgccg tattgtgtga gatagctttt
    23401 cgcggcgcac accaccattg gcatcgcgcc aagacggcgg gaaaacaggc tggaatcctg
    23461 caacgcgccg acgcggatca ccacatccag accgtcggca atcaggtcgg gggctggaat
    23521 tccggtaacc agattgacgc tcaaacctgg gtattctttc agcattttgg ctgtcagccc
    23581 ggcgagaaca ttttgtgcca tagttgaaga acagccaatg cgtagcgtcc cgatgggggt
    23641 gttattgaag gcatacagtt gctcatgaac atcctgcact tcatgaagca tacgacggca
    23701 gccctggtag taaattctgc cggcttcggt caggccaatg ctgcgtgtgc tacggtttaa
    23761 cagctttacc tgcaactcat cttccagttt tgacaccgtc tgactgatgg acgaaacgct
    23821 catctgtagc tgtctggcgg cggcggtaaa agagccaaat tcaactactt tggcaaacac
    23881 cgacatgcgt tttagtcgtt ccattattca ctctgactta aaagtgattt agatcacata
    23941 atatagataa cagcataaca gttacgctaa tatattaaat atcaatctac agcaatgttg
    24001 ctctcgcccg gctttccatg cccttctctg cggcgacaga tgctgaaaat aataacgcct
    24061 gctctctcta tacaaccaag gtcaacatga gtctgtttcc cgttatcgtg gtgtttgggc
    24121 tgtccttccc accgatattt tttgaattgc ttttatcact ggcgattttc tggctggtgc
    24181 gccgggtact tgtgccaaca ggtatctacg actttgtctg gcatccggcg ttgttcaaca
    24241 ccgcgctcta ttgctgcttg ttttatttga tatcgcgact gttcgtttga ggttgaagtg
    24301 aaaacactaa taagaaaatt ctcccgtacg gccatcacgg tcgtattagt cattctggcc
    24361 ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat ccccctggac gcgtgacgcg
    24421 cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt ctggactcat tacccaggtg
    24481 aatgttcatg ataaccagct ggtgaaaaaa ggacagatac tgttcaccat cgaccagccg
    24541 cgctatcaaa aggcgcttga ggaagcgcaa gccgatgttg cttattatca ggtactggca
    24601 caggagaaac gccaggaggc cggacgtcgt aaccgtctcg gtgtgcaggc gatgtctcgc
    24661 gaagagatcg accaggccaa caacgtacta caaacggttc tgcatcagtt agcgaaagcg
    24721 caagcgaccc gcgatctggc aaaactggat cttgaacgca cggtgatccg cgcgccagcg
    24781 gatggctggg tgaccaacct caacgtctat accggtgagt ttattactcg tggatcaacg
    24841 gcggttgcgc tggtgaaaca gaactctttc tatgtactgg cctatatgga agaaactaag
    24901 ctggaagggg tgcgccctgg gtatcgtgca gagatcacgc cgcttggcag taacaaagtg
    24961 ctgaaaggga ctgttgatag tgttgccgct ggggtcacca acgccagcag cacgcgtgac
    25021 gataaaggga tggcgaccat cgactctaac cttgaatggg tgcgtttggc gcaacgtgtt
    25081 ccggttcgta ttcgtctcga caaccagcaa gagaacatct ggcctgcggg caccactgct
    25141 acagtggtgg tcactggcaa acaagatcgc gacgaaagcc aggattcgtt cttccgtaaa
    25201 atggcccatc gcctgcgtga gtttggttaa tcacgatggg tattttctcc attgctaacc
    25261 aacatattcg ctttgcggta aaactggcga ccgccattgt actggcgctg tttgttggct
    25321 ttcacttcca gctggaaacg ccacgctggg cggtactgac agcggcgatt gttgccgccg
    25381 gtccggcctt tgctgcggga ggtgaaccgt attctggcgc tattcgctat cgtggctttt
    25441 tgcgcatcat cggcacattt attggctgta ttgctggact ggtgatcatc attgcgatga
    25501 tccgcgcacc attattgatg attctggtgt gctgtatctg ggccggtttt tgtacctgga
    25561 tatcctcgct ggtacgaata gaaaactcgt atgcgtgggg gctggccggt tataccgcgc
    25621 tgatcattgt gatcaccatt cagccggaac cattgcttac gccgcagttt gccgtcgaac
    25681 gttgtagcga gatcgttatc ggtattgtgt gtgcgattat ggcggatttg ctcttttctc
    25741 cgcgatcgat caaacaagaa gtggatcgag agctggaaag tttgctggtc gcgcaatatc
    25801 aattaatgca actctgtatc aagcatggcg atggtgaagt tgtcgataaa gcctggggcg
    25861 acctggtgcg acgcaccacg gcgctacaag gcatgcgcag caacctgaat atggaatctt
    25921 cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa tacgctatcg ctgacgctga
    25981 ttacccaatc ctgcgaaact tatcttattc agaatacgcg cccggagttg atcactgata
    26041 ctttccgcga attttttgac acgccggtag aaaccgcgca ggacgttcac aagcagctca
    26101 aacgcctgcg gagagttatc gcctggaccg gggaacggga aacgcctgtc accatttata
    26161 gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg cggcgttatc agtaacacaa
    26221 aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc ggaagtcaaa gtagagtcag
    26281 ccgaacgtca tcatgcgatg gttaacttct ggcgaaccac actttcctgc attctgggca
    26341 cgcttttctg gctgtggacg ggctggactt ccggcagtgg tgcaatggtg atgattgcgg
    26401 tagtgacgtc actggcaatg cgtttgccga atccacgcat ggtggcgatc gactttatct
    26461 acgggacgct ggccgcgctg ccgttagggc tgctctactt tttggtgatt atccctaata
    26521 cccaacagag catgttgctg ctgtgtatta gcctggcagt gctgggattt ttcctctgta
    26581 tagaagtaca gaaacggcga ctgggctcga tgggggcact ggccagcacc ataaatatta
    26641 tcgtgctgga taacccgatg actttccatt tcagtcagtt tctcgacagc gcattagggc
    26701 aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct ggtgcgggat aaatcgcgcg
    26761 acaggactgg acgtgtactg cttaatcagt ttgtttctgc cgctgtttcc gcgatgacta
    26821 ccaatgtggc acgtcgtaaa gagaaccacc tcccggcact ttatcagcag ctgtttttgc
    26881 tgatgaataa gttcccaggg gatttgccga aatttcgcct ggcgctgacg atgattatcg
    26941 cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga ggatttatcg gcgtttcacc
    27001 gacaaatgcg ccgcacagca gaccatgtga tatctgcccg tagcgatgat aaacgtcgcc
    27061 ggtactttgg ccagttgctg gaagaacttg aaatctacca ggaaaagcta cgcatctggc
    27121 aagcgccacc gcaggtgacg gaaccggtac atcggctggc ggggatgctc cataagtatc
    27181 aacatgcgtt gaccgatagt taagtcaaaa ccgacgccaa aagcgtcggt tttttcatgg
    27241 ctatacttag cgatgcacgg cagaactcgc cgcgaaacgt gacggtggca acagatgaat
    27301 atttatacct ttgattttga tgagattgag agtcaggagg atttttatcg tgactttagc
    27361 caaacctttg gtctggcgaa agataaggtg cgcgatctcg actcactatg ggatgtgtta
    27421 atgaacgatg tcctgccgct accacttgag attgaatttg ttcatctggg agagaaaacg
    27481 cgtcgccgtt ttggcgcgtt aatattgcta tttgatgagg cagaggaaga gctggaaggg
    27541 catttgcgtt ttaatgttcg tcattagcgc aaaaaaaagc ccccgaaccg ggggcaatat
    27601 cgtcggacaa gacgatgagg gtttatttgt acagttcagc cgtagcgtgc caggtgtcac
    27661 cgctacgagc ttcagtaatc tggtaggccg ttgcgccttt ctcttccgct tttttgttca
    27721 gcatttcacg tatatccatt ggcgaagacg ccacaccact tacggatacg gtcccgattg
    27781 cttcacggtt ttgtgcttgt gcagcatcaa tggagtcggc agcgaatgca ccgaaagaga
    27841 gaacagaaag tacgcttaat gcagcaacag tggttttgat tttcatgatt tttacctcga
    27901 cataatcttt ttgctgggtc tttgtttcgt gacccttatc acaaaatcaa gtatacacta
    27961 attattggac taattaata
//
LOCUS       DAPGRN010000060        27579 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPGRN010000060 DAPGRN010000000
VERSION     DAPGRN010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.060"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            439..1578
                     /gene="wza"
                     /locus_tag="RHW33_002686"
     CDS             439..1578
                     /gene="wza"
                     /locus_tag="RHW33_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV1646173.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1584..2027
                     /gene="wzb"
                     /locus_tag="RHW33_002687"
     CDS             1584..2027
                     /gene="wzb"
                     /locus_tag="RHW33_002687"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV1646174.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2030..4192
                     /gene="wzc"
                     /locus_tag="RHW33_002688"
     CDS             2030..4192
                     /gene="wzc"
                     /locus_tag="RHW33_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV1646175.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4285..5124
                     /gene="wcaA"
                     /locus_tag="RHW33_002689"
     CDS             4285..5124
                     /gene="wcaA"
                     /locus_tag="RHW33_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV1646176.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVSALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKDCLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5127..5615
                     /gene="wcaB"
                     /locus_tag="RHW33_002690"
     CDS             5127..5615
                     /gene="wcaB"
                     /locus_tag="RHW33_002690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV1646177.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5612..6829
                     /gene="wcaC"
                     /locus_tag="RHW33_002691"
     CDS             5612..6829
                     /gene="wcaC"
                     /locus_tag="RHW33_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV1646178.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6804..8021
                     /gene="wcaD"
                     /locus_tag="RHW33_002692"
     CDS             6804..8021
                     /gene="wcaD"
                     /locus_tag="RHW33_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV1646179.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFQVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8032..8778
                     /gene="wcaE"
                     /locus_tag="RHW33_002693"
     CDS             8032..8778
                     /gene="wcaE"
                     /locus_tag="RHW33_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV1646180.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8794..9342
                     /gene="wcaF"
                     /locus_tag="RHW33_002694"
     CDS             8794..9342
                     /gene="wcaF"
                     /locus_tag="RHW33_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV1646181.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9371..10492
                     /gene="gmd"
                     /locus_tag="RHW33_002695"
     CDS             9371..10492
                     /gene="gmd"
                     /locus_tag="RHW33_002695"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV1646182.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10495..11460
                     /gene="fcl"
                     /locus_tag="RHW33_002696"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10495..11460
                     /gene="fcl"
                     /locus_tag="RHW33_002696"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV1646183.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRNELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHKVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11463..11942
                     /gene="gmm"
                     /locus_tag="RHW33_002697"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11463..11942
                     /gene="gmm"
                     /locus_tag="RHW33_002697"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV1646184.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVSEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11939..13162
                     /gene="wcaI"
                     /locus_tag="RHW33_002698"
     CDS             11939..13162
                     /gene="wcaI"
                     /locus_tag="RHW33_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV1646185.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVAEADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13165..14601
                     /gene="cpsB"
                     /locus_tag="RHW33_002699"
     CDS             13165..14601
                     /gene="cpsB"
                     /locus_tag="RHW33_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV1646186.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRTGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14794..16164
                     /gene="cpsG"
                     /locus_tag="RHW33_002700"
                     /gene_synonym="manB"
     CDS             14794..16164
                     /gene="cpsG"
                     /locus_tag="RHW33_002700"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV1646187.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQLLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16219..17613
                     /gene="wcaJ"
                     /locus_tag="RHW33_002701"
     CDS             16219..17613
                     /gene="wcaJ"
                     /locus_tag="RHW33_002701"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV1646188.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17615..19093
                     /gene="wzxC"
                     /locus_tag="RHW33_002702"
     CDS             17615..19093
                     /gene="wzxC"
                     /locus_tag="RHW33_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV1646189.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKILSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFMLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGGSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19369..20649
                     /gene="wcaK"
                     /locus_tag="RHW33_002703"
     CDS             19369..20649
                     /gene="wcaK"
                     /locus_tag="RHW33_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV1646190.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKHSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            20646..21866
                     /gene="wcaL"
                     /locus_tag="RHW33_002704"
     CDS             20646..21866
                     /gene="wcaL"
                     /locus_tag="RHW33_002704"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV1646191.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPAGKVAKLHHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGKLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMLGFKPSHEVKAMLDEADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALEQRLAAFSQLDTDELAPVVKRARDKVEHDFNQ
                     QVINRDLASLLQAL"
     gene            21877..23271
                     /gene="wcaM"
                     /locus_tag="RHW33_002705"
     CDS             21877..23271
                     /gene="wcaM"
                     /locus_tag="RHW33_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV1646192.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGKTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGKEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKATLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23446..24339
                     /gene="galF"
                     /locus_tag="RHW33_002706"
     CDS             23446..24339
                     /gene="galF"
                     /locus_tag="RHW33_002706"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV1646193.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGCS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIIEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGYSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            24712..25797
                     /gene="rfbB"
                     /locus_tag="RHW33_002707"
     CDS             24712..25797
                     /gene="rfbB"
                     /locus_tag="RHW33_002707"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV1646194.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFVHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KKLPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYCEQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            25797..26696
                     /gene="rfbD"
                     /locus_tag="RHW33_002708"
     CDS             25797..26696
                     /gene="rfbD"
                     /locus_tag="RHW33_002708"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDV1646195.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPD
                     GVAETIRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWVIHY
                     STDYVFPGSGDRPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPDVAGLYHLVAS
                     GTTTWYDYAALVFEEARKADIPLALNKLNAVPTTAYPTPACRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26754..>27579
                     /gene="rfbA"
                     /locus_tag="RHW33_002709"
     CDS             26754..>27579
                     /gene="rfbA"
                     /locus_tag="RHW33_002709"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV1646196.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALILGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIETRQGLKVSCPEEIAYRKGFISDEQMQIIASK"
BASE COUNT         6859 a         6716 c         7434 g         6570 t
ORIGIN      
        1 aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc cgaattgtta
       61 ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag agccaatatt
      121 caattaccat aatgtcacct tatattcgga agttaatttc cgcaataacc ctttctcatc
      181 aacgattgta tgcagagagg gtttgaaaaa ttaagctaaa tcagtgtatt ggtagctaaa
      241 aagccagggg cggtagcgtg tctggatgcc tgaaagacca gtctgaatta ttctgtcaat
      301 agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaatttgt attcaattaa
      361 ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata attactctgc
      421 caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt attggtgtca
      481 gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac gatgggcaaa
      541 gacgtcatca aacagcagga cgctgatttc gatctcgaca aaatggtgaa tgtttatccg
      601 ctgaccccgc gcctgattga ccaattacgc ccacgcccga atgtagcgcg ccccaatatg
      661 acgctggaaa gtgagatcgc gaattaccag tatcgcgtcg ggccgggcga cgttctgaat
      721 gtcaccgtct gggatcaccc ggaactcacc acgccagccg gtcagtaccg cagctccagc
      781 gacaccggca actgggtaca gcctgacggc actatgtttt acccgtatat cggcaaggtc
      841 cacgtagtcg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg cttagcgacg
      901 tacatcgctg acccgcaggt ggatgttaat atcgccgcct tccgctcgca aaaggcctat
      961 atctcaggtc aggtgaataa atccggtcaa caggcgatca ccaacgtacc actgaccatt
     1021 ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg caacgtggtg
     1081 ctaacacaca atggtcgtga agaacgcatt tctttgcagg cgctgatgca aaacggcgac
     1141 ctcaatcaga accgactgct ttaccccggc gatattctct acgtgccgcg taatgatgat
     1201 ctgaaagtgt ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat ggactttagc
     1261 ggcatgaccc tgactgaagc cctgggcaat gccgaaggta tcgacatgac cacctccaac
     1321 gccagcggca tctttgtcat tcgtccgctg aaaggcgagg gcgggcgtaa cggcaagatt
     1381 gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtgatggc gacagaattc
     1441 cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtttcccg ctggaaccgt
     1501 ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga tacagccagc
     1561 gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt gtcggcaata
     1621 tttgccgttc cccgacggcg gaacgcttac tgcaacgtta tcacccggag ctgaaagtgg
     1681 agtccgctgg actcggcgcg ctggtcggta agggcgctga tcctaccgct atcagcgtcg
     1741 ccgcagaaca tcaactgtct ctggaaggtc actgtgcacg gcaaatcagc cgccgcctgt
     1801 gtcgcaacta tgacctgatt ttgaccatgg aaaagcgcca tatcgaacgc ttatgcgaga
     1861 tggcacctga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac gaatgtgaaa
     1921 tccccgatcc gtatcgcaaa agccgggaaa cgtttgcagc ggtgtacaca ttacttgaac
     1981 ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa tgacagaaaa
     2041 agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg gtcgcctggt
     2101 cggcaccgtc attgaagcgc gctggtgggt gattggcatc accgctgtat ttgccctctg
     2161 tgctgtggtt tacactttct tcgccacgcc gatttatagt gccgacgcac tggtacaaat
     2221 cgaacaaaac agcggcaatt cgttagtgca ggacattggc tcggcattag ccaacaaacc
     2281 gcctgcatcg gacgccgaga tccagttgat tcgttcgcgt ctggtgcttg gtaaaacggt
     2341 ggacgatctc gacctcgata ttgcagtgag caaaaacacg ttcccgattt tcggtgcggg
     2401 ctgggatcgt ttgatgggac gtcagaacga gacggtgaaa gtgaccacct ttaaccgccc
     2461 gaaagagatg gcggatcagg tgtttacgct taatgtgctg gacaacaaaa actacaccct
     2521 gagcagcgac ggcggcttta gcgcccgggg gcaagcgggc cagatgctga aaaaagaagg
     2581 cgtcacgctg atggttgaag ccattcacgc cagcccgggc agtgagttta ccgtcaccaa
     2641 atactccacg ctggggatga tcaaccaact gcaaaacagc ctgacggtaa cggagaacgg
     2701 caaagacgct ggcgttttga gcctgactta taccggtgaa gatcgtgaac agatccgcga
     2761 cattcttaac agcatcgccc gtaactatca ggaacaaaat attgagcgca aatcggcgga
     2821 agcgtcgaaa agcctcgcct tcctcgcgca acagttaccg gaagtacgta gccgcttgga
     2881 tgttgccgaa aacaaactga atgccttccg tcaggataaa gattctgttg atctgccgct
     2941 ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcccagttga acgaactgac
     3001 ctttaaagag gcggaaatct ccaagctgta caccaaagtt cacccggcgt accgcacgct
     3061 gctggagaaa cgtcaggcgc tggaagacga aaaagccaaa cttaacggtc gcgtaacggc
     3121 gatgccgaaa acccagcagg agattgtccg tctgacccgc gatgtcgagt ctggtcagca
     3181 ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag ccagcaccgt
     3241 cggcgatgtg cgcattgttg acccggcaat cactcagcct ggtgtgctaa aaccgaagaa
     3301 agggctgatt atccttgggg cgattattct tggcctgatg ctctctatcg tgggtgtgct
     3361 gctgcgctcg ttgtttaatc gcggcatcga aagcccgcag gtgctggaag aacacggtat
     3421 cagcgtctat gccagcatcc cgttgtcgga atggcagaaa gcgcgcgata gcgtcaaaac
     3481 catcaaaggg attaaacgct ataaacagag ccagctactg gcggtgggga atccaaccga
     3541 tctggcgatt gaagccatcc gcagtctgcg taccagtctg cacttcgcga tgatgcaggc
     3601 gcagaacaat gtgttgatga tgaccggggt tagcccgtca atcggtaaaa cctttgtctg
     3661 cgccaacctg gcggcggtga tcagccagac caataaacgc gtgttgttga tcgactgcga
     3721 tatgcgcaaa ggctacaccc acgagctgtt gggcaccaat aacgtcaatg gcctgtcgga
     3781 aattctgatt ggtcagggcg atattactac cgcagctaaa ccgacctcta ttgccaaatt
     3841 tgacctgatc ccgcgcggtc aggtgccgcc aaatccttct gaactgttga tgagcgaacg
     3901 ctttgccgaa ctggtgaact gggcgagtaa aaactatgac ctggtgttga ttgatacgcc
     3961 gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgtcggaa ccacattaat
     4021 ggtggcgcgt tatgcggtca acacattgaa agaagtggaa accagtctga gccgctttga
     4081 gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc gcgccagcgc
     4141 gtatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat aacgacgcct
     4201 gcattcgcac cgccccgtag gccggataag gcgttcacgc cgcatccggc aagcaaacca
     4261 gctcataagc cgggagaaca acctatgaaa gacaatccgc tgatctcaat ctatatgccg
     4321 acctggaacc gccaacaact ggcgattcgg gcgataaaat cggtcctgcg ccaggactac
     4381 agtaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca actgcaacag
     4441 tacgtcagcg ccctcaacga tccgcgtatc acctacattc acaacgacat taacagcggg
     4501 gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatacat taccgggatc
     4561 gatgacgatg atgaatggac gcccaaccgt ctgagcgtct tcctcgccca taaacagcaa
     4621 ctggtcacac acgccttttt gtacgctaac gactacgttt gccagggcga agtctattcc
     4681 caaccggcaa gcttgccgct gtatcccaaa tcgccgtatt cacgccgcct gttctacaaa
     4741 cgcaatatca ttggcaatca ggtctttacc tgggcatggc gtttcaaaga ttgtttgttc
     4801 gataccgaac taaaagcggc acaggattac gacatcttcc tgcggatggt ggtggagtat
     4861 ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca cggtgaaatg
     4921 cagatcacct cgtcaccgaa aaaattctcc ggatacttcc acttttaccg caagcacaaa
     4981 gacaaatttg accgtgccag taaaaaatat cagctgttta ccctctacca gatccgcaat
     5041 aaacgtatga cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg caaacgtctg
     5101 gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgcgccaac agctggagtt
     5161 tacgtccgtg ctgcatggtt ctagcgtatc gcgtcgctca tttttgttcg gtgtggcgca
     5221 aaaagaacgt cctcaacaat atttgggcgg cccctctgct ggtgctgtat cgcattatca
     5281 ccgaatgctt tttcggttat gaaatccagg ctgccgcgac cattggccgc cgctttacta
     5341 tccatcacgg ttacgccgta gtgatcaata aaaacgtggt ggcgggggat gatttcacca
     5401 ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa catggcatgt ccacatattg
     5461 gcaacggcgt cgaactcggt gccaacgtta ttattcttgg tgatatcacg cttggtaaca
     5521 acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac gcgctggtgg
     5581 tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ttgcaattta atgtgcgact
     5641 ggcggaaggc ggggcagcag gtgtggcgtt agatctccac cagcgtgcgc tgcaacaggg
     5701 gctagcgtca cattttgtct acggttacgg caaaggcggc aaagagagcg tcagccatca
     5761 aaactatccg caggtcatca aacatacgcc gcggatgact gcaatggcga atattgtcct
     5821 gtttcgtctg tttaatcgcg atctgtttgg caatttcaat gagttatatc gcaccattac
     5881 tcgtacaccg ggtccggtgg tcctgcattt tcatgtgctg cacagctact ggctaaatct
     5941 taagagcgtg gtgcgctttt gcgaaaaggt gaaaaaccat aaaccggacg tcactctggt
     6001 ctggacgctg cacgaccact ggagcgttac cggacgctgc gcctttaccg acggttgtga
     6061 aggctggaaa acgggctgcc agaaatgccc gaccttaaat aactatccgc cggtgaagat
     6121 tgatcgcgcg caccagctgg tggcgggcaa acgccagtta ttccgtgaga tgctggcgct
     6181 gggctgtcag tttatttccc ccagccagca tgtggctgac gctttcaata gcctgtacgg
     6241 tccagggcgt tgccggatta tcaataatgg tattgatatg gcaaccgaag cgattctggc
     6301 ggatctgcct ccggtgcgcg aaacccaggg caagccgaaa atcgcggtgg tggcgcatga
     6361 cctgcgttac gacggcaaga ctaaccagca actggtgcgt gagatgatgg cgctgggcga
     6421 caaaattgaa ctgcatacct ttggtaagtt ctcgccgttc accgctggca acgtggttaa
     6481 tcacggcttt gaaactgaca agcgcaagtt gatgagcgcg ctcaatcaaa tggatgcgct
     6541 ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgc tatcgattgg
     6601 cgtgccggtg atcgccaccc atagcgatgc ggcgcgggaa gtgctgcaaa aatccggtgg
     6661 taaaaccgtc agtgaagaag aggtgctgca actggtgcag ttaagcaaac cggaaatcgc
     6721 gcaggcgata tttggtacca cgctggctga gttcagccaa cgcagccgcg ccgcctacag
     6781 tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc tacctgctgc
     6841 tgccgctgat ctacctgctg gttaacgtca aaatcgccca gcttggtgaa agtttcccca
     6901 tcactatcgt cactttttta cctgtcttgt tactgctgtt tttagaacgc atcagcgtta
     6961 aaaaattgat gattgcctta gggattggtg cgggactcac ggcgttcaac tatctgtttg
     7021 gtcagtcgtt ggatgccagt aaatacgtca cttcaaccat gctgtttgtc tatattgtga
     7081 tcattattgg catggtgtgg agtattcgct ttaaaacgat ttcgccacac aaccatcgca
     7141 agatattacg tttcttttat ctggtggtcg ggctggtggt ggcgctggcg gcggtggaga
     7201 tggcgcaaat tatcctcacc ggtggcagca gtattatgga gtcgatttcg aaatatctga
     7261 tttacagcaa cagctatgta ctgaatttca ttaaattcgg cggcaagcgc acgacagcac
     7321 tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc agcatcaaac
     7381 agtttggtat caaaacgcct aaaaccgatg ctatgattct cgcagggata atattatccg
     7441 gatcgttttc aggggttatg acctttatcc tgttttattt gctggagtgg gcatttcaat
     7501 atctgaataa agaggcgatt aagaaaaagt taccgttggc attgatttct ctggctgtat
     7561 tccaggttgg tgtggtaatc gcgtttcctt atatttccac ccgtctgggc gatttaggta
     7621 cggaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc ggttattctt
     7681 tgacccatat tgacggtgta gtcagatttg gctcacttta tgaatacgtc gcatcattcg
     7741 gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat ctgctgatta
     7801 tttatttttc ctggttcgca gtgtttttat cgctatggta catggggaaa gtgataaaaa
     7861 tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat ctttttactc
     7921 cggtgtcgct gttttttacc ggttcgatat ttagcccgga atatgcattt ttaatcgtct
     7981 gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta agaataagaa catgttgctt
     8041 agcataatca ctgtcgcgtt tcgtaacctc gaagggatag tcaaaacaca tgcctcgctg
     8101 gcgcatctgg cgcaggcgga agatatcagc ttcgaatgga ttgttgtcga tggcggttcc
     8161 aatgacggca cccgtgagta tctggaaaat ctcaatggta tctataacct acgctttgtc
     8221 agcgagccag ataacggtat ctacgacgcc atgaacaaag gcattacgat ggcacaaggc
     8281 aagttcgcgt tgtttctcaa ttcgggcgat atttttcatc aggatgccgc aaattttgtc
     8341 cgtaagttaa aaatgcaaaa agataacatg atgatcaccg gcgatgcgct gctggatttt
     8401 ggcgacgggc ataaaattaa acgtagcgcc aaaccgggct ggtatattta tcacagcctg
     8461 cccgccagtc atcaggcgat atttttccct gtatccggct tgaaaaaatg gcgttatgac
     8521 ctggaatata aagtttcttc tgattacgcg ctggcagcta aaatgtataa agcaggttat
     8581 gcatttaaaa aactcaatgg cctggtgtct gaattttcta tgggtggggt atcaaccacc
     8641 aataatatgg aattgtgtgc tgacgccaaa aaagtccaac ggcagatatt acatgtgcct
     8701 ggcttctggg cagaattatc ctggcattta cgccaacgta ctacctcaaa gacgaaagcc
     8761 ttatataaca aaagctgaat ataagggaaa acgatgcagg atttaagtgg attctcggtg
     8821 ccgaaagggt tccggggtgg caacgctatt aaagtgcaat tgtggtgggc agtacaggca
     8881 acattatttg cctggtcgcc acaagtattg tatcgctggc gggctttttt attacgttta
     8941 ttcggcgcaa aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat tacctatccg
     9001 tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg aggtcaattt atataccctc
     9061 ggtgaaataa ccattggcgc acattcggtg atatcgcaaa aaagttattt atgcaccggt
     9121 agccacgacc atgcaagtca acatttcacc attaacgcca cgcctattgt gattggcgag
     9181 aaatgctggc tggcaaccga tgtctttgtt gccccaggcg tcacaatcgg cgacggcacc
     9241 gtcgtggggg cgcgaagcag tgtttttaaa tcgcttccgg caaatgtggt ttgccggggg
     9301 aatcccgcag tggtgatacg cgaacgcgtt gaaactgaat aaattcaaaa aaaatacaga
     9361 ggaataagac atgtcaaaag tcgctctcat caccggtgta accggacaag acggttctta
     9421 cctggcagag tttctgctgg aaaaaggtta cgaggtgcat ggtattaagc gtcgtgcatc
     9481 gtcattcaac accgagcgcg tggatcacat ttatcaggat ccgcacacct gcaacccgaa
     9541 attccatctg cattatggcg acctgagtga tacctccaac ctgacgcgca ttttgcgtga
     9601 agtgcagccg gatgaagtgt acaacctggg ggcaatgagc cacgttgcgg tttcttttga
     9661 gtcaccggaa tataccgcag acgtcgatgc gatgggtacg ctgcgcctgc tggaggcgat
     9721 ccgcttcctc ggtctggaaa agaaaacccg tttctatcag gcttccacct ctgaactgta
     9781 tggtctggtg caggaaattc cgcagaaaga aaccacgccg ttctatccac gctctccgta
     9841 tgcggtcgcc aaactgtatg cctactggat caccgttaac taccgcgaat cctacggcat
     9901 gtatgcctgt aacggtattc tcttcaacca tgaatccccg cgccgcggcg aaaccttcgt
     9961 tacccgcaaa atcacccgcg caatcgccaa catcgcccag gggctggagt cgtgcctgta
    10021 cctcggcaat atggattccc tgcgtgactg gggccatgcc aaagactacg taaaaatgca
    10081 gtggatgatg ctgcaacagg aacaaccgga agatttcgtt attgctaccg gcgttcagta
    10141 ctccgtgcgt cagttcgtgg aaatggcggc agcacagctg ggcatcaaac tgcgctttga
    10201 aggcacgggc gttgaagaga agggtattgt ggtttccgtc accgggcatg acgcgccggg
    10261 cgtgaaaccg ggtgatgtga ttatcgccgt tgatccgcgt tacttccgtc cggctgaagt
    10321 tgaaacgctg ctcggtgacc cgaccaaagc gcacgaaaaa ctgggctgga aaccggaaat
    10381 caccctcaga gagatggtgt ctgaaatggt ggctaatgac cttgaagcag cgaaaaaaca
    10441 ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg ctggagtcat aagcatgagt
    10501 aaacaacgca tttttatcgc tggccatcgc gggatggtcg gttctgccat caggcggcag
    10561 ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc gcaacgagct gaacctgctg
    10621 gacagccgcg ccgtgcatga tttctttgcc agcgaacgta ttgaccaggt ctatctggcg
    10681 gcggcgaaag tgggcggcat tgttgccaac aacacgtatc cggcggattt catctatcag
    10741 aacatgatga ttgagagcaa catcattcac gccgcgcatc agaacgacgt gaacaaactg
    10801 ctgtttctcg gatcgtcctg catctacccg aaactggcaa aacagccgat ggcagaaagc
    10861 gagttgttgc agggcacgct ggagccgacc aacgaacctt atgccattgc caaaatcgcc
    10921 gggatcaaac tgtgcgaatc ttacaaccgc cagtatggac gcgattaccg ctcggtcatg
    10981 ccgaccaacc tgtatggccc gcatgacaac ttccacccga gtaattcgca tgtgatccct
    11041 gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg cgccggacgt ggtggtgtgg
    11101 ggcagtggta caccgatgcg cgaatttctg catgtcgatg atatggcggc ggcaagcatt
    11161 catgtgatgg agctggcgca caaagtctgg ctggagaaca cccagccgat gctgtcgcac
    11221 attaacgtcg gcacgggcgt tgactgcact atccgcgagc ttgcgcaaac catcgccaaa
    11281 gtggtgggtt acaaaggtcg ggtggttttt gatgccagta aaccggatgg tacgccgcgc
    11341 aaactgctgg atgtgacgcg cctgcatcag cttggctggt atcacgaaat ctcactggaa
    11401 gcggggcttg ccagcactta ccagtggttc cttgagaatc aagaccgctt tcgggggtaa
    11461 tgatgttttt acgtcaggaa gactttgcca cggtagtgcg ctccacaccg cttgtctctc
    11521 tcgactttat tgttgagaac agtcgcggcg agtttctgct tggcaaaaga accaaccgcc
    11581 cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca gaaagacgaa acgctggaag
    11641 ccgcatttga gcggctgacg atggcggaac tgggtctgcg tctaccgata acagcaggcc
    11701 agttttacgg tgtctggcag cacttttatg acgataactt ctccggcacg gatttcacca
    11761 cgcactatgt ggtgctcggt tttcgcttca gagtatcgga agaagagctg ttactgccgg
    11821 atgagcagca tgacgattac cgctggctga cgccggatgc actgctcgcc agtgacaatg
    11881 ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg tgccggagta cccggattat
    11941 gaaaatactg gtctacggca ttaactactc gccggagtta accggcatcg gcaaatacac
    12001 cggagagatg gttgaatggc tggcggcgca aggtcatgag gtgcgggtta ttaccgcacc
    12061 gccttactac ccgcagtggc aggtgggcga gaactattcc gcctggcggt acaaacgaga
    12121 agagggggcc gctacggtgt ggcgctgccc gctgtacgtg ccaaaacagc cgagcaccct
    12181 gaaacgcctg ttgcatctcg gcagttttgc cgtcagcagt ttctttccgc tgatggcgca
    12241 acgtcgctgg aagccggagc gcattatcgg cgtagtgcca acgctgtttt gcacgccggg
    12301 aatgcgtctg ctggcgaaac tctctggcgc gcgtaccgtg ctgcatattc aggattacga
    12361 agtggacgcc atgctggggc tgggccttgc cggaaaaggc aaaggcggca aagtggcaca
    12421 gctggcgacg gcgttcgaac gtagcggact gcataacgtc gataacgtct ccacgatttc
    12481 gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg gcggaaaacg tcatcttctt
    12541 ccccaactgg tcggaaattg cccgttttca gcatgtcgca gaggccgatg ttgatgccct
    12601 tcgtaaccag cttggcctgc cggataacaa aaaaatcatt ctttattccg gcaatattgg
    12661 tgaaaagcag gggctggaaa acgttattga agctgccgat cgtctgcgtg atgaaccgct
    12721 gatttttgcc attgtcgggc agggcggcgg caaagcgcgg ctggaaaaaa tggcgcagca
    12781 gcgtggactg cgcaacatgc aatttttccc gctgcaatcg tatgacgctt tacccgcact
    12841 gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc ggcgcggcag atgccgtatt
    12901 gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac gcggtgatta ccgcagaggc
    12961 ccacacagaa ctggggcagc tttgcgaaac ctttccgggc attgcggttt gcgtagaacc
    13021 ggaatcggtc gaggcgctgg tggcggggat ccgtcaggcg ctcctgctgc ccaaacacaa
    13081 cacggtggca cgtgaatatg ccgaacgcac actcgataaa gagaacgtgt tacgtcaatt
    13141 tataaatgat attcggggat aattatggcg cagtcgaaac tctatccagt tgtgatggca
    13201 ggtggctccg gtagccgctt atggccgctt tcccgcgtac tttaccccaa gcagttttta
    13261 tgcctgaaag gcgatctcac catgctgcaa accaccatct gccgcctgaa cggcgtggag
    13321 tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct ttattgtcgc ggaacagctg
    13381 cgtcaactga acaaacttac cgagaacatt attctcgaac cggcagggcg aaacacggca
    13441 cctgccattg cgctggcggc gctggcggca aaacgtcata gcccggagag cgacccgtta
    13501 atgctggtat tggcggcgga tcatgtgatt gccgatgaag acgcgttccg tgccgccgtg
    13561 cgtaatgcca tgccatatgc cgaagcgggc aagctggtga ccttcggcat tgtgccggat
    13621 ctaccagaaa ccggttatgg ctatattcgt cgcggtgaag tgtctgcggg tgagcaggat
    13681 acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga atctggaaac cgcccaggcc
    13741 tatgtggcaa gcggcgaata ttactggaac agcggcatgt tcctgttccg caccggacgc
    13801 tatctcgaag aactgaaaaa atatcgtccg gatattctcg atgcctgtga aaaagcgatg
    13861 agcgccgtcg atccggatct cgattttatt cgtgtggatg aagaagcgtt tctcgcctgc
    13921 ccggaagagt cggtggatta cgcggtcatg gaacgtacgg cagatgctgt tgtggtgccg
    13981 atggatgcgg gctggagcga tgtcggttcc tggtcttcat tatgggagat cagcgcccac
    14041 accgccgagg gcaacgtttg ccacggcgat gtgattaatc acaaaactga aaacagctat
    14101 gtgtacgccg aatctggcct ggtcaccacc gtcggggtga aagatttggt ggtagtgcag
    14161 accaaagatg cagtgctgat tgccgaccgt aacgcggtgc aggatgtgaa aaaagtggtc
    14221 gagcagatca aagccgacgg tcgccatgag catcgggtgc atcgcgaagt gtatcgtccg
    14281 tggggcaaat atgactctat cgacgcgggc gaccgctacc aggtgaaacg catcaccgtg
    14341 aaaccgggcg aaggcttgtc ggtacagatg caccatcacc gtgcggaaca ctgggtggtt
    14401 gtcgcgggaa cggcaaaagt caccattgac ggtgatatca aactgcttgg tgaaaacgag
    14461 tccatttata taccgctggg ggcgacgcac tgcctggaaa acccggggaa aattccgctc
    14521 gatttaattg aagtgcgctc cggctcttat ctcgaagagg atgatgtggt gcgcttcgcg
    14581 gatcgctacg gacgggtgta aacgtcgcat caggcgctgt ttgtcggatg cggcgtgaac
    14641 gccttatccg acctacggtt cggttttgta ggcctgataa gacgcggcag cgtcgcatca
    14701 ggcaatgaat gcgaaaccgc ggtgtaaata acgacaaaaa caaaattggc cgcttcggtc
    14761 agggccaact attgcctgaa aaagggtaac gacatgaaaa aattaacctg ctttaaagcc
    14821 tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag atattgcctg gcgcattggt
    14881 cgcgcctatg gcgaatttct caaaccgaaa accattgtgt taggcggtga tgtccgcctc
    14941 accagcgaaa ccttaaaact ggcgctggca aaaggtttac aggatgcggg cgtcgatgtg
    15001 ctggatatcg gcatgtccgg cactgaagag atctatttcg ccacgttcca tctcggcgtg
    15061 gatggcggca tcgaagttac tgccagccat aatccgatgg attacaacgg catgaaactg
    15121 gtgcgcgagg gggctcgccc gatcagcggt gataccggac tgcgcgatgt ccagctcctg
    15181 gcagaagcca acgacttccc tcccgtcgat gaaaccaaac gcggtcgcta tcagcaaatc
    15241 aacctgcgtg atgcatacgt tgatcacctg ttcggttata tcaacgtcaa aaacctcacg
    15301 ccgctcaagc tggtgattaa ttccgggaac ggcgcggcgg gtccggtggt ggacgccatt
    15361 gaagcccgct ttaaagccct cggcgcacca gtggaattga tcaaagtgca caacacgccg
    15421 gacggcaatt tccccaacgg aattcctaac ccgctgctgc cggaatgccg cgacgacacc
    15481 cgcaatgcgg tcatcaaaca cggcgcggat atgggtattg cctttgatgg cgattttgac
    15541 cgctgcttcc tgtttgacga aaaagggcag tttattgaag gctactacat tgtcggtttg
    15601 ctggcagaag cgttcctcga aaaaaatccc ggcgcgaaga tcatccacga tccgcgtctc
    15661 tcctggaaca ccgttgatgt ggtgactgcc gcaggtggca cgccggtaat gtcgaaaacc
    15721 ggacacgcct ttattaaaga acgtatgcgc aaggaagacg ccatctacgg tggcgaaatg
    15781 agcgctcacc attacttccg tgatttcgct tactgcgaca gcggcatgat cccgtggctg
    15841 ctggtcgccg aactggtgtg cctgaaaggg aaaacgctgg gcgaactggt gcgcgaccgg
    15901 atggctgcgt ttccggcaag cggtgagatc aacagcaaac tggcgcaccc cgttgaggcg
    15961 attaaccgcg tcgaacagca ttttagccgt gaggcgctgg cggtggatcg caccgatggc
    16021 atcagcatga cctttgccga ctggcgcttt aacctgcgct cctccaacac cgaaccggtg
    16081 gtgcggttga atgtggaatc gcgcggcgat gtaccgctga tggaagcgcg aacgcgaact
    16141 ctgctgacgt tgctgaacga gtaatgtcgg atcttccctt accccactgc gggtaagggg
    16201 ctaataacag gaacaacgat gacaaatcta aaaaagcgcg agcgagcgaa aaccaatgca
    16261 tcgttaatct ctatggtgca acgcttttca gatatcacca tcatgtttgc cggactatgg
    16321 ctggtttgcg aagtgagcgg actgtcattc ctctacatgc acctgttggt ggcgctgatt
    16381 acgctggtgg tgttccagat gctgggcggc atcaccgact tttatcgctc atggcgcggt
    16441 gttcgggcag cgacagaatt tgccctgttg ctacaaaact ggacgttaag cgtgattttc
    16501 agcgccggac tggtggcgtt caacaatgat ttcgacacgc aactgaaaat ctggctggcg
    16561 tggtatgggc tgaccagcat cggactggtg gtttgccgtt catgtattcg tattggggcg
    16621 ggctggctgc gtaatcatgg ctataacaag cgtatggtcg ccgtagcggg ggatttagcc
    16681 gccggacaaa tgctgatgga gagcttccgt aatcagccgt ggttagggtt tgaagtggtg
    16741 ggcgtttacc acgacccgaa accgggtggc gtttctaacg actgggcggg caatctgcaa
    16801 caactggtcg aggatgcgaa agcgggcaag attcataacg tctatatcgc gatgcagatg
    16861 tgcgacggcg cgcgagtgaa aaaactggtc catcaactgg cggacaccac ctgttcggtg
    16921 ctgctgatcc ccgatgtctt taccttcaac attctccatt cacgtctcga agagatgaac
    16981 ggcgttccgg tggtgccgct gtatgacacg ccgctttccg gggttaaccg cctgctaaaa
    17041 cgtgcggaag acattgtgct ggcgacgctg atcttgctgc tgatctcccc ggtgctgtgc
    17101 tgtattgcgc tggcggtgaa actcagttca cctgggccgg ttattttccg ccagactcgc
    17161 tatggcatgg atggcaagcc gatcaaagtg tggaagttcc gttccatgaa agtgatggag
    17221 aacgacaaag tggtgaccca ggcgacgcag aacgatccgc gcgtcaccaa agtggggaac
    17281 tttctgcgcc gcacctcgct ggatgaactg ccgcagttta tcaatgtgct gaccggcggg
    17341 atgtcgattg tcgggccacg tccgcacgca gtagcgcata acgaacagta tcgacagctc
    17401 attgaaggct acatgctgcg ccataaggtg aaaccgggca ttaccggctg ggcgcagatt
    17461 aacggctggc gcggcgaaac cgacacactg gagaaaatgg aaaaacgcgt cgagttcgac
    17521 cttgagtaca tccgcgaatg gagcgtctgg ttcgatatca aaatcgtttt cctgacggta
    17581 ttcaaaggct tcgttaacaa agcggcatac tgacatgagc ttacgtgaaa aaaccatcag
    17641 cggcgcgaag tggtcggcga ttgccacggt gatcatcatc ggcctcgggc tggtgcagat
    17701 gaccgtgctg gcgcggatta tcgacaacca ccagttcggt ttgcttaccg tgtcgctggt
    17761 gattatcgcg ctggcagata cgctttctga cttcggtatc gctaactcga ttattcagcg
    17821 aaaagaaatt agccaccttg aactcaccac gctgtactgg ctgaacgtcg ggctggggct
    17881 tgtggtgtgt gtggcggtgt ttttgttgag tgatctcatt ggtgacgtgc tgaacaaccc
    17941 ggacctggca ccgttgatta aaatattgtc gctggcgttt gtggtgatcc cccacgggca
    18001 gcagttccgc gcgctgatgc aaaaagagct ggagttcaac aaaatcggca tgatcgaaac
    18061 cagcgcggtg ctggcgggct tcacttttac ggtggttagc gcccatttct ggccgctggc
    18121 gatgaccgcg atcctcggtt atctggtcaa tagtgcggta agaacgctgc tgtttggcta
    18181 ctttggccgc aaaatttatc gccctggtct gcatttctcg ctggcgtctg ttgcaccgaa
    18241 cttacgcttt ggtgcctggc tgacggcgga cagcatcatc aactatctca ataccaacct
    18301 ttcgacgctg gtgctggcgc gtattctcgg cgcgggcgtg gcagggggat acaacctggc
    18361 gtacaacgtg gccgttgtgc cgccgatgaa gctgaaccca atcatcaccc gcgtgttgtt
    18421 tccggcattc gccaaaattc aggacgatac cgaaaagctg cgcgttaact tctacaagct
    18481 gctgtcggta gtggggatta tcaactttcc ggctctgctg gggctgatgg tggtgtcgaa
    18541 taactttgta ccgctggtct ttggtgagaa gtggaacagc attattccgg tgctgcaatt
    18601 gctgtgtgtg gtgggtctgc tgcgttcggt gggtaacccg attggttcgc tgctgatggc
    18661 gaaagcgcgg gtcgatatca gctttaaatt caacgtattc aaaacatttc tgtttattcc
    18721 ggcgattgtt attggtgggc agatggcggg cgcaatcggc gtcacgctcg gtttcatgct
    18781 ggtgcagatt atcaacacca ttctgagtta cttcgtgatg attaaaccgg ttcttggttc
    18841 cagttatcgc cagtacatcc tgagtttgtg gctgccattt tatctctcgc tgccgacgct
    18901 ggggggcagt tatgcgctgg gcattgtgct gaaagggcaa ctggcgctgg ggatgctgct
    18961 ggcggtgcaa atagccgcgg gcgtgctggc gtttgtggtg atgattgtgc tgtcgcgcca
    19021 tccgctggtg gtggaagtga agcgccagtt ttgtcgcagc gaaaaaatga aaatgctttt
    19081 acgggcgggg tgagttgttg ttttcggttc cgacaacggt tgccgggtgc agcgtaaaac
    19141 gccttatcca tcctacggtt ctgtgctcgg gacctgtagg cctgataaga ctcgtcagcg
    19201 tcgcatcagg cactgaatgc cggatgcggc gtgaacgcct tatccggcct acggttctgt
    19261 gctcgggtct tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaaa aaacggcaac
    19321 gaacacaaca aacacagcct cacctggcat caaatttatg aggaaaaaat gaaattactt
    19381 attctgggca accacacttg cggcaaccgc ggcgacagcg ccatcttgcg cggcttactt
    19441 gatgccatca acatcctcaa tccacacgcc gaagtggacg tcatgagccg ctatccggtc
    19501 agttcttcct ggctgctcaa ccgcccggta atgggtgatc cgctgttcct gcaaatgaaa
    19561 caacacaaca gcgcggcggg cgttgtcggg cgcgttaaaa aagtcctccg tcgccgctat
    19621 cagcaccagg tattgctctc acgcgtcacc gacactggca agctgcgtaa tatcgccatc
    19681 gcccagggat tcaccgactt cgtgcgcctg ctgtcaggtt acgacgccat tatccaggtc
    19741 ggcggatcgt tttttgtcga tctctacggc gtgccgcagt ttgaacatgc actttgcacg
    19801 tttatggcga aaaagccgct gtttatgatt ggtcacagtg ttggcccgtt ccaggatgag
    19861 caatttaacc aactggcgaa ctacgttttt ggtcactgcg acgcgctgat cctgcgcgaa
    19921 tcggtcagcc ttgatctgat gaaacacagc aatatcacca ccgcaaaagt ggaacatggc
    19981 gtcgataccg cgtggctggt cgatcaccac acagaagact tcaccgccag ctatgccgtt
    20041 caacactggc tggacgttgc cgcacaacag aaaacggtgg ccattaccct gcgcgaactg
    20101 gcaccgttcg acaaacgtct cggcaccact caacaagcgt atgaaaaagc ctttgccggg
    20161 gtggtcaatc gcattctcga cgaaggctat caggtgattg cgctctccac ctgtacgggc
    20221 atcgacagct ataacaaaga cgaccgcatg gtggcgctca atcttcgcca gcacatcagc
    20281 gatcctgccc gttaccacgt ggtgatggat gaactcaacg atctggaaat gggcaaaatt
    20341 ctgggggcct gtgaactcac cgtcggtacg cgcctgcact ctgccattat ctcgatgaac
    20401 tttgccactc cggcgattgc catcaactat gaacataaat ccgctgggat catgcaacag
    20461 ctgggactgc cagagatggc aattgatatc cgtcatttat tagacggcag cctgcaagcg
    20521 atggttgcgg ataccttagg tcagcttccg gcgctgaacg cacgacttaa cgaagctgtc
    20581 agtcgcgaac gtcagacggg aatgcagatg gtgaaatctg tgcttgaacg catcggggag
    20641 gcaaaatgaa ggtcggcttc tttttactga aatttccgct gtcgtcagaa accttcgttc
    20701 ttaaccaaat cactgcgttt attgatatgg gctttgaagt ggagattgtc gcgctgcaaa
    20761 aaggcgacac acaaaacacc cacgcggcat ggacgaaata caaccttgcc gccagaaccc
    20821 attggttaca ggacgaacct gcgggcaaag tggcgaaact gcaccaccgc gccagccaga
    20881 ccttacgcgg cattcatcgt aaaaatacct ggcaggcgct caacctcaaa cgctatggtg
    20941 ctgagtcgcg gaacctgatt ttgtctgcca tttgcggtca ggtcgcaaca ccatttcatg
    21001 ccgatgtctt tatcgctcat tttggccctg cgggggtaac cgcagcaaaa ctacgggaac
    21061 tgggtgtcat tcgcggcaaa atcgctacta tcttccacgg cattgatatc tccagccggg
    21121 aagtgctcaa ccattacact cccgaatatc aacaactgtt tcgccgtggc gacctgatgt
    21181 taccgataag cgatttgtgg gccggaaagc tgcaaaaaat gggctgcccg agggaaaaaa
    21241 tcgccgtatc gcgcatgggc gtggacatga cgcgctttag cccgcgtccg gtgaaagcgc
    21301 ccgcaacacc gctggaaatc atttccgtcg cacgcttaac cgagaaaaaa ggcctgcatg
    21361 tggcgatcga agcctgtcgt cagttgaaag agcagggcgt ggcatttcgc tatcgcatcc
    21421 tcggcattgg cccgtgggaa cgacgcctgc gcaccctcat cgaacaatat caactggaag
    21481 atgtggtaga gatgctgggc tttaaaccga gccacgaagt gaaagcgatg ctagacgaag
    21541 cggatgtctt cctgttgcca tcggtaacgg gtgcggacgg cgatatggaa ggcattccgg
    21601 tagcgctgat ggaggcgatg gcggtcggca ttccggtggt ttctactctg catagcggaa
    21661 ttcctgaact ggtggaggcc gataaatccg gctggctggt gcctgagaac gatgctcgag
    21721 cactggagca acgactggcg gcgtttagcc aactggacac cgacgaactg gcgccggtcg
    21781 tcaaacgtgc gcgcgataaa gtcgaacatg attttaacca gcaggtgatc aatcgagacc
    21841 tcgccagctt gctgcaagct ttatagagga gaacgcatgc catttaaaaa actctcccga
    21901 cgcaccttcc tgacggcaag ctcggcgctt gccttcctcc ataccccttt cgctcgcgcg
    21961 cttcccgccc gacaaagcgt taacattaac gactacaacc cacacgactg gatcgcctca
    22021 tttaaacaag ccttcagcga agggaaaacg gtcgtcgtgc ccgccggatt ggtttgtgac
    22081 aatatcaaca ccggcatctt cattcctcct ggaaaaacgt tacacatcct tggaagcctg
    22141 cgcggcaacg gcagagggcg ttttgtctta caggatggca gccaggtgac agggaaggag
    22201 ggcggcagca tgcataatat caccctggat gtgcgtggtt ctgactgcac cattaaaggg
    22261 ctggcaatga gcggctttgg cccggtaacg cagatttata tcggcggcaa aaacaaacgg
    22321 gtcatgcgca acctgaccat cgataacctc actgtcagcc acgctaatta cgccatctta
    22381 cgccagggat ttcataacca gattatcggt gccaacatca ccaactgtaa gttcagcgac
    22441 ttacagggcg acgccattga atggaacgtg gcaattaacg acagtgatat tttgatatct
    22501 gaccatgtca tcgagcgcat caactgtacc aacggcaaaa tcaactgggg cattggcata
    22561 ggccttgcgg ggagtactta tgataataac tacccggaag atcaggcagt gaaaaacttt
    22621 gtcgtggcga atatcacggg atcggattgt cggcagttga tccatgttga aaatggtaaa
    22681 cattttgtta ttcgtaatat caaagcccgc aatatcacac cggacttcag taagaaagca
    22741 ggtattgata acgccacggt tgctatttac ggttgtgaca atttcgtgat tgataatatt
    22801 gaaatgatta atagtgccgg gatgttaatc ggctatgggg taattaaagg caaatatctc
    22861 tcgataccgc aaaatttccg agtgaataat attcaactgg ataacaccca tcttgcttac
    22921 aaattgcgcg gcatccaaat ctctgccggg aatgctgtct cctttgtggc gctgactaac
    22981 attgagatga agcgtgcgtc gctggagtta cacaacaaac cgcaacacct ttttatgcga
    23041 aatatcaagg tgatgcagga atcctcagtt ggaccagcat tgagtatgaa ctttgacatg
    23101 cgcaaagacg ttcgaggcgt gtttatggcg aaaaaagcaa cactgctgtc tcttgcaaat
    23161 gttcatgcgg tgaatgaaaa agggcaaagc tccgtcgata ttgacaggat taatcaccat
    23221 attgttaatg tggaaaagat taactttaga ttgccggaac ggagggagta gatttgcgac
    23281 cattcctgga aaaatggagt catacttagg aacaatgcta ctgcaatcca caacgaagcg
    23341 gcgtaacatc acaagtaatt cagtaatcaa ttcagggtaa ttgatgctgg cgaaaaaaat
    23401 cgaacaagct ataattcagc aaccatttta caggtgggtg aaataatgac gaatttaaaa
    23461 gcagttattc ctgtagcggg tctagggatg catatgttgc ctgccactaa ggcgattccc
    23521 aaagagatgc taccgatcgt cgacaagcca atgattcagt acattgttga cgagattgtg
    23581 gctgcaggga tcaaagaaat cctcctggta actcacgcgt ccaagaacgc ggtcgaaaac
    23641 cacttcgaca cctcttatga attagaatct ctccttgaac tgcgcgtgaa gcgtcaactg
    23701 ctggcggaag tgcaatctat ctgcccgccg ggcgtgacca ttatgaacgt gcgtcagggc
    23761 gaacctttag gtttaggcca ctccattttg tgtgcacgcc ccgccattgg tgacaacccg
    23821 tttgtcgtgg tactgccaga cgttgtgatc gacgatgcca gcgccgaccc gctacgttac
    23881 aaccttgctg ccatgattgc gcgcttcaac gaaacgggct gcagccaggt gctggcaaaa
    23941 cgtatgccgg gtgacctctc tgaatactcc gtcattcaga ccaaagaacc actggatcgt
    24001 gaaggtaaag ttagccgcat tattgaattt atcgaaaaac cggatcagcc acaaacgctg
    24061 gactcagaca tcatggccgt tggtcgctat gtgctttctg ccgatatttg gccggaactt
    24121 gaacgcacgc agcctggtgc atggggacgt attcagttga ctgatgccat tgccgaactg
    24181 gcgaaaaaac agtccgttga tgccatgctg atgaccggct acagctacga ctgcggtaaa
    24241 aaaatgggtt atatgcaggc gtttgtgaag tatggactgc gtaacctaaa agaaggggcg
    24301 aagttccgta aagggattga gaagctgtta agcgaataat gaaaatctga ccggatgtaa
    24361 cggttgataa gaaaatcata acggcagtga agattcgcgg ggaaagtaat ttgttgcgaa
    24421 ttttcctgcc gttgttttat ataaacaatc agaataacaa agagttagca ataggatttt
    24481 cgtcaaagtt ttccaggatt ttccttgttt ccagagcgga ttggtaagac aattagcgta
    24541 tggatttttc gggtttggcg cgagtgggta acgctcgcca catcgtaggc atgcatgcag
    24601 tgctctggta gctgtaaagc caggggcggt agcgtgtgtt gatacctcta ttaattaaac
    24661 tgagggccgc ttatttcaca gcatgctctg aagtaatatg gaataaatta agtgaaaata
    24721 cttgttactg gtggtgcagg atttattggt tctgcggtag ttcgtcacat tataaataat
    24781 acgcaggata gtgttgttaa tgtcgataaa ttaacgtacg ccggaaacct ggaatcactt
    24841 gctgatgttt ctgattctga acgctatgtt tttgtacatg cggatatttg cgatgctgct
    24901 gcaatggcgc ggatttttgc tcagcatcag ccggatgcag tgatgcacct ggctgctgaa
    24961 agccatgtgg atcgttccat cactggccct gcggcattta ttgaaaccaa tattgttggt
    25021 acatatgtcc ttttggaagc cgctcgcaat tattggtctg ctcttgatag cgacaagaaa
    25081 aacagcttcc gttttcatca tatttctact gacgaagtct atggtgattt gcctcatcct
    25141 gacgaggtaa ataataaaga agaattaccc ttatttactg agacgacagc ctacgcgcca
    25201 agcagccctt attccgcatc caaagcatcc agcgatcatt tagtccgcgc gtggaaacgt
    25261 acctatggtt taccgaccat tgtgactaat tgctctaaca attatggtcc ttatcatttc
    25321 ccggaaaaat taattccatt ggttattctc aatgctctgg aaggtaaaaa attacctatt
    25381 tatggtaaag gggatcaaat tcgcgactgg ttgtatgttg aagatcatgc gcgtgcgtta
    25441 tataccgtcg taaccgaagg taaagcgggt gaaacttata acattggtgg acacaacgaa
    25501 aagaaaaaca tagatgtagt gctcactatt tgtgatttgc tggatgagat tgtaccgaaa
    25561 gagaaatctt attgtgagca aatcacttat gttgccgatc gtccgggaca cgatcgccgt
    25621 tatgccattg atgctgagaa gattggtcgc gaattgggat ggaaaccaca ggaaacgttt
    25681 gagagcggga ttcggaagac agtggaatgg tacctgtcca atacaaaatg ggttgataat
    25741 gtgaaaagtg gtgcctatca atcgtggatt gaacagaact atgagggccg ccagtaatga
    25801 atatcctcct ttttggcaaa acagggcagg taggttggga actacagcgt gctctggcac
    25861 ctttgggtaa tttgattgct cttgatgttc actctactga ttattgcggt gattttagta
    25921 atcctgacgg tgtagctgaa accataagaa gcattcggcc tgatattatt gtcaacgcag
    25981 ccgctcacac cgcagtagac aaagcagaat cagaaccgga gtttgcacaa ttacttaacg
    26041 caacaagtgt cgaagcgatt gcgaaagcag caaatgaagt tggagcttgg gttatacact
    26101 actccaccga ttatgttttc cctggcagtg gtgacaggcc atggctggaa acggatgcaa
    26161 cagcgccgct aaatgtttac ggtgaaacta agctagctgg ggaaaaagcg ttacaagaac
    26221 attgcgcaaa gcatcttatt ttccgtacca gctgggtata cgctggtaaa ggaaataact
    26281 ttgccaaaac gatgttgcgt ctggcaaaag aacgcgaaga gttggctgtg ataaacgatc
    26341 aatttggcgc accaacgggt gctgagctgc tggccgattg tacagcacat gccattcgtg
    26401 tcgcactgaa taaaccggat gtcgcaggct tgtaccatct ggtagccagt ggtaccacga
    26461 cctggtacga ttatgctgcg ctggtttttg aagaggcgcg caaagcagat attcccctcg
    26521 cactcaacaa actcaatgcg gtaccaacga cagcctatcc tacaccagct tgtcgtccac
    26581 ataactctcg acttaataca gaaaaatttc agcagaattt tgcgcttgtc ttgcctgact
    26641 ggcaggttgg tgtgaaacgc atgctcaacg aattatttac gactacagca atttaatagt
    26701 ttttacatct tgttcgtgat gatggagcaa gatgaattaa aaggaatgat gaaatgaaaa
    26761 cgcgtaaggg tattatttta gcgggtggtt ctggtactcg tctttatcct gtgactatgg
    26821 cagtcagtaa acagctgcta ccgatttatg ataaaccgat gatctattac ccgctctcta
    26881 cactgatgtt ggcgggtatt cgcgatattt tgattattag tacgccacag gatactcctc
    26941 gttttcaaca actgctgggt gacggtagcc agtgggggct aaatcttcag tacaaagtgc
    27001 aaccgactcc agatgggctt gcgcaggcat ttattatcgg tgaagagttt attggtggtg
    27061 atgattgtgc tttgattctt ggtgataata tcttttacgg tcacgatctg ccgaagttaa
    27121 tggatgccgc tgttaacaaa gaaagtggtg caacggtatt tgcctatcac gtaaatgatc
    27181 ctgaacgcta tggtgtcgtt gagtttgata aaaacggtac tgcaatcagc ctggaagaaa
    27241 aaccgttaca acccaaaagt aattatgcgg taaccgggct ttatttctat gataacgatg
    27301 ttgtcgaaat ggcgaaaaat cttaagcctt ctgcccgcgg tgaactggaa attaccgata
    27361 ttaaccgtat atatatggaa caagggcgtt tatctgttgc catgatgggc cgtggttatg
    27421 cgtggttaga cacggggaca catcagagcc tgattgaggc aagcaacttc attgccacaa
    27481 ttgaaacacg tcaggggcta aaagtttctt gtccagaaga gattgcgtat cggaaagggt
    27541 ttattagtga tgagcaaatg cagataatag cctctaaat
//
LOCUS       DAPGRN010000061        27495 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPGRN010000061 DAPGRN010000000
VERSION     DAPGRN010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.061"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..357)
                     /locus_tag="RHW33_002710"
     CDS             complement(<1..357)
                     /locus_tag="RHW33_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646197.1"
                     /translation="MIEGMIMRIFVFFISALLSFNLAAEECKFSFNESELISSIGIAP
                     VKQEIIKDEGITKRRQYEFRRELSSEEMLSDDADEKYEPQFYISVYNPSCPQKVIVWF
                     FKDNKNTMGLSNEVLAG"
     gene            complement(439..2271)
                     /locus_tag="RHW33_002711"
     CDS             complement(439..2271)
                     /locus_tag="RHW33_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV1646198.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKVMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDATGSNPFMIN
                     KASVPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFR"
     gene            complement(2413..2682)
                     /locus_tag="RHW33_002712"
     CDS             complement(2413..2682)
                     /locus_tag="RHW33_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDV1646199.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VKLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(2682..3038)
                     /locus_tag="RHW33_002713"
     CDS             complement(2682..3038)
                     /locus_tag="RHW33_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDV1646200.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIISLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(3038..4534)
                     /locus_tag="RHW33_002714"
     CDS             complement(3038..4534)
                     /locus_tag="RHW33_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDV1646201.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVATIASSIQDAINAVPTLPFTASSSA
                     GVVTLTARHKGFCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4518..4688)
                     /locus_tag="RHW33_002715"
     CDS             complement(4518..4688)
                     /locus_tag="RHW33_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HDV1646202.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDDKL"
     gene            complement(4697..5257)
                     /locus_tag="RHW33_002716"
     CDS             complement(4697..5257)
                     /locus_tag="RHW33_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646203.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            complement(5254..5760)
                     /locus_tag="RHW33_002717"
     CDS             complement(5254..5760)
                     /locus_tag="RHW33_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646204.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(5735..6145)
                     /locus_tag="RHW33_002718"
     CDS             complement(5735..6145)
                     /locus_tag="RHW33_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDV1646205.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VAFRAWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGEFTQSHGGSNGDSLFSR"
     gene            complement(6142..6465)
                     /locus_tag="RHW33_002719"
     CDS             complement(6142..6465)
                     /locus_tag="RHW33_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDV1646206.1"
                     /translation="MLLKMEEIKLQLRLDDDFSDEDELLELLGKAAQSRTENFLNRKL
                     YATADDRPADDPDGLVISDDVKLALLLLVSHFYENRSTVTDVEKMELPMSFNWLVVPY
                     RLIPL"
     gene            complement(6544..7773)
                     /locus_tag="RHW33_002720"
     CDS             complement(6544..7773)
                     /locus_tag="RHW33_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDV1646207.1"
                     /translation="MKLHELKQKRNTIATDMRALNEKIGDNAWTEEQRTEWNKAKSEL
                     EALDERIAREEELRRQDQAYIESNEEEQRQNLDPENNPQQDEKRAQVFDKWMRHGASE
                     LTSEERKALRELRAQGVAQDEKGGYTVPETFLAKVVEKMKSYGGIASVAQILTTSDGR
                     TMEWATADGTSEVGVLLGENEEAGEEDTDFGMGSLGALKMTSKIIRVSNELLQDSAID
                     MEAYLARRIAERIGRGEARYLIQGTGAGTPKQPKGLVASVTGTTQTAAANTVKWQEIL
                     ALKHSIDPAYRRGPKFRLAFNDNTLKLISEMEDGQGRPLWLPDIVGVAPASVLNVPYV
                     IDQEIDDIGAGKKFMFCGDFDRFIIRRVRYMILKRLVERYAEYDQTGFLAFHRFDCIL
                     EDTSAIKALVGKGSVGG"
     gene            complement(7784..8386)
                     /locus_tag="RHW33_002721"
     CDS             complement(7784..8386)
                     /locus_tag="RHW33_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDV1646208.1"
                     /translation="MNDREIRCYSGEVRAERHDDNPAHIIGYGSVFDCRSELIFGSFR
                     EIIRPGAFDDVLGDDVRALFNHDPNFILGRSAAGTLNLSVDERGLRYDIQAPETQTIR
                     DLVLAPMQRGDINQSSFAFRVARDGEEWYQDEDGVVIREITRFSRLLDVSPVTYPAYQ
                     EADSAVRSMKAWQEARNSGALQKAINQRMARERVLTLLNA"
     gene            complement(8379..9605)
                     /locus_tag="RHW33_002722"
     CDS             complement(8379..9605)
                     /locus_tag="RHW33_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1646209.1"
                     /translation="MLLDALFRSKSLENPSTPITGDAVDTDGLFRADVYVSPETAMKL
                     AAVYSCIYVLSSSLAQMPLHVMRRHNGKVEPARDHPAFYLVHDEPNTWQTSYKWRELK
                     QRHILGWGNGYTWVKRNRRGEVISLDCCMPWETTLMNTGGRYTYGLYNEYGAFAISPD
                     DMIHIRALGNNQKMGLSPIMQHAETIGMGMSGQKYTESFFSGNARPAGIVSVKSGLNK
                     ESWGWLKEQWQKASQALRRQENKTMLLPADLDYKALTVSPVDAQIIDMMKLNRSMIAG
                     IFNIPAHMINDLEKATFSNISAQAIQFVRYTMMPWVTNWEQELNRRLFTRAELAAGYY
                     VRFNLTGLLRGTPQERAQFYHFAITDGWMSRNEARAFEDMNPVEGLDEMLVSVNAANP
                     AGDFKPPKNDEGKTNE"
     gene            complement(9595..9756)
                     /locus_tag="RHW33_002723"
     CDS             complement(9595..9756)
                     /locus_tag="RHW33_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646210.1"
                     /translation="MKSLIADVIGLTGFGLLTCGVYLQFGMAPALILSGALLLVGALV
                     MVRRGTRAA"
     gene            complement(9753..11249)
                     /locus_tag="RHW33_002724"
     CDS             complement(9753..11249)
                     /locus_tag="RHW33_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDV1646211.1"
                     /translation="MPITLEPWQLFVICCAFGWVNKGTRLRRFREVYTEIPRKNGKSA
                     ISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPARLMCKRTPMLTEAFGIEVNAS
                     NMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHATDALYTTMLTGMGARRQPLMWA
                     ITTAGYNIEGPCYDKRREVIEMLNGSVPNDELLGIIYTVDEGDDWTDPQVLEKANPNI
                     GVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSARSAYFNLVSWQSCEDKSLTLE
                     QFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYYSVAPRFWVPYDTVYSVEKNED
                     RRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAANKISPVSESPIDPFGATGLSHD
                     LADEDLNPVTIVQNFANMSDPMKELEAAIESGRFHHDGNPIMTWCIGNVVGKNMPGND
                     DLVKPVKEQAENKIDGAVALIMTIGRAMLKEPDDFLSSLDPDDDLLIL"
     gene            complement(11483..11977)
                     /locus_tag="RHW33_002725"
     CDS             complement(11483..11977)
                     /locus_tag="RHW33_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDV1646212.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(12103..12453)
                     /locus_tag="RHW33_002726"
     CDS             complement(12103..12453)
                     /locus_tag="RHW33_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1646213.1"
                     /translation="MPPRTPKACRVRGCRHTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(12546..12866)
                     /locus_tag="RHW33_002727"
     CDS             complement(12546..12866)
                     /locus_tag="RHW33_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="negative regulator GrlR"
                     /protein_id="HDV1646214.1"
                     /translation="MKDGIYFVVFRSNQRDFGNGTVVVKNNAVNGGDFGFTYQGKVDG
                     SQLILRVSQHDLNATSVFPGVKNFELSLSLQERGRDYLLNGSVVGMPQMQISISAKYI
                     GDLI"
     gene            complement(13113..13505)
                     /locus_tag="RHW33_002728"
     CDS             complement(13113..13505)
                     /locus_tag="RHW33_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDV1646215.1"
                     /translation="MKMSYWALILTFIACVAGGLVWSANHYHGKFLEEQKRADAAEQR
                     ADSTEAITANVLRTMAITNIIQEANQHAKQQIALESQRTQKDIKVAVADDDCASRPVP
                     AAAADRLRKYANSLRPGSGSSVTSQPDG"
     gene            complement(13502..14116)
                     /locus_tag="RHW33_002729"
     CDS             complement(13502..14116)
                     /locus_tag="RHW33_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDV1646216.1"
                     /translation="MNQQLFQKAAGISAGLAARWFPHIDAAMKEFGITAPADQAMFIA
                     QVGHESMGFSAVVENFNYTPSALVATFGKRITQQQADALGRTSGHAARQDAIANLVYS
                     NRLGNKAPGDGWKYRGRGLIQITGLHNYRICGAALKLDLVTSPEQLEQELQAARSAAW
                     FYTSKGCMIYGADINRVTRIINGGLNGIEDRKVRYNKARAALLV"
     gene            complement(14116..14397)
                     /locus_tag="RHW33_002730"
     CDS             complement(14116..14397)
                     /locus_tag="RHW33_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV1646217.1"
                     /translation="MELNDPTATINALLCACVVITLMFYRRGDSRHRPWVSRLAWLIT
                     VTYSAVPLAYLCGIYPHSSWPIIVANTIFLSVLVAVRGNVARLVDHLRH"
     gene            complement(14384..14770)
                     /locus_tag="RHW33_002731"
     CDS             complement(14384..14770)
                     /locus_tag="RHW33_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV1646218.1"
                     /translation="MSDPISGTGLAGGALTGASVYGLLTGTDYGVVFGAFAGAVFYIA
                     TAADLSASRRLAYFIVSYIAGILCSGLVGSKLANLTGYSDKPLDAIGAVIVSALAVKI
                     LTFLNNQDIGSLVALITRRGGSGGAK"
     gene            complement(14861..15457)
                     /locus_tag="RHW33_002732"
     CDS             complement(14861..15457)
                     /locus_tag="RHW33_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646219.1"
                     /translation="MTWQNVPYAFEKTTGELTLVIEKLPPIEISSSFPFETLITALAG
                     VIAAGITGWVAYRAIKENFALATLQAHLNTNKELAQQIRFAGAEHVTDVIMLASTFEQ
                     WHLVGNKNMDILAKGVFPEEIQVPIKAAEISKNKLLLLIRPDEEGCKLITLTADLQKA
                     LKVCFTKGYFTPEEKKSFIDAQNAFIFGCHEYINQSLS"
     gene            complement(15564..16142)
                     /locus_tag="RHW33_002733"
     CDS             complement(15564..16142)
                     /locus_tag="RHW33_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDV1646220.1"
                     /translation="MINPSEVGKSGEMVRLRTLESIWIQGKLRMWGRWSYIGGGSGGN
                     MFNQLLASGKITKTAINEALRRMKIAGITKPELEAYLREILNSKNKSGLAFCSDEEGL
                     LVDGVIASVLMNDDYRSLYSVIVDRYRLRKSKLQMANELQAKHPDWPLITCRRRIDTW
                     LSLAESILYAPVCDAFGTNSDRFKLQSEQESA"
     gene            complement(16157..17146)
                     /locus_tag="RHW33_002734"
     CDS             complement(16157..17146)
                     /locus_tag="RHW33_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV1646221.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHAV
                     TMPELCWWLIRNDLAGALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(17154..17951)
                     /locus_tag="RHW33_002735"
     CDS             complement(17154..17951)
                     /locus_tag="RHW33_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDV1646222.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDIVH"
     gene            complement(17971..18360)
                     /locus_tag="RHW33_002736"
     CDS             complement(17971..18360)
                     /locus_tag="RHW33_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV1646223.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPGGKIEITISKYEKTAGAAA"
     gene            complement(18357..18683)
                     /locus_tag="RHW33_002737"
     CDS             complement(18357..18683)
                     /locus_tag="RHW33_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDV1646224.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENGRLRAAHWLH
                     ERGLKV"
     gene            complement(18680..19333)
                     /locus_tag="RHW33_002738"
     CDS             complement(18680..19333)
                     /locus_tag="RHW33_002738"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV1646225.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEVCGEAFLAQVRQQAEKLVREMAA"
     gene            complement(19333..19827)
                     /locus_tag="RHW33_002739"
     CDS             complement(19333..19827)
                     /locus_tag="RHW33_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV1646226.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQSVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMAAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(19824..20816)
                     /locus_tag="RHW33_002740"
     CDS             complement(19824..20816)
                     /locus_tag="RHW33_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646227.1"
                     /translation="MSLLMTSQPIVINRDLACRIGLNEAIVLQQLHYWLNETNSGTEH
                     GGIRWVYNTTEQWLEQFPFWSESTLKRTFASLKSLGVLRREQLNKSKRDMTNFYTINY
                     ESELLEEVKVNESIRSKCTSPSGQSDLMDGRKMTRSIGSKRHAVIGSKWPNDLTENTT
                     EITTENKTSSRPDASQPDTQVAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKK
                     IIALYEQAAECDGEVVRPKEPNWTAWANEIRLMCVQDGRNHKQICEMYSRVSRDPFWC
                     RNVLSPSKLREKWDELSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAGFRG"
     gene            complement(20773..20985)
                     /locus_tag="RHW33_002741"
     CDS             complement(20773..20985)
                     /locus_tag="RHW33_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HDV1646228.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEPVNDIPAHCDKS"
     gene            complement(21161..21718)
                     /gene="ymfL"
                     /locus_tag="RHW33_002742"
     CDS             complement(21161..21718)
                     /gene="ymfL"
                     /locus_tag="RHW33_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HDV1646229.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(21711..21971)
                     /locus_tag="RHW33_002743"
     CDS             complement(21711..21971)
                     /locus_tag="RHW33_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1646230.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEVSRG"
     gene            22069..22761
                     /locus_tag="RHW33_002744"
     CDS             22069..22761
                     /locus_tag="RHW33_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1646231.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDVKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            23484..23846
                     /locus_tag="RHW33_002745"
     CDS             23484..23846
                     /locus_tag="RHW33_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646232.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            23912..24736
                     /locus_tag="RHW33_002746"
     CDS             23912..24736
                     /locus_tag="RHW33_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDV1646233.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            24864..25400
                     /gene="yfdR"
                     /locus_tag="RHW33_002747"
     CDS             24864..25400
                     /gene="yfdR"
                     /locus_tag="RHW33_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV1646234.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATETYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            25391..25753
                     /locus_tag="RHW33_002748"
     CDS             25391..25753
                     /locus_tag="RHW33_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646235.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVIPKAPEAGA"
     gene            25753..26373
                     /locus_tag="RHW33_002749"
     CDS             25753..26373
                     /locus_tag="RHW33_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDV1646236.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALVSLEAEPVA
                     WLHSDNGLGIPAITRSKNIADSWLSKGWYVQPLYIAKPVPVVPDARPSLNNGIVGFDE
                     GWNACRAAMLNGAEPVSQTYKLNELSGNSPVTPDGWISCSERMPNDKQYVWCWGKFYG
                     WAECDTFEGYYDWSKNKWWAVTDDVEESASKVTHWMPLQEPPHEVK"
     gene            26853..>27495
                     /locus_tag="RHW33_002750"
     CDS             26853..>27495
                     /locus_tag="RHW33_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HDV1646237.1"
                     /translation="MKHLNDFPKRDRNHRIERLAISAFEHFLSDSNDFFIQSKDINDY
                     GVDYQLEITTNGQATNIRLFVQLKGTEQELNNDGSVSISVNRANLNYLLMHPYSFYVC
                     FHAPSDTLKITFADSVIRSYEQSEKKWIEQETITVRFIDNISESRLHEIAEVARLNAL
                     DNRNARIEFITKPISNVSAVINKTIPKIHIPEDKKLAHELLNSLYENNHDDVIS"
BASE COUNT         6890 a         7398 c         6406 g         6801 t
ORIGIN      
        1 accagcaagg acctcattac ttaaacccat tgtgttttta ttgtctttga aaaaccaaac
       61 aataaccttt tgtgggcatg atggattata aacagatata taaaactgcg gctcatattt
      121 ttcatcagcg tcatcactaa gcatttcttc agaagataat tctcttctga attcatattg
      181 ccgccgctta gttattcctt catcctttat tatctcttgc ttaactggtg caatacctat
      241 agaagagatt aattctgact cattaaagct gaacttacac tcttccgcag ccaagttaaa
      301 agataaaagt gcagatataa aaaaaacaaa gatacgcata atcatccctt caatcatttg
      361 taaggaatga ttatattaac tacttaaatc tgaaaaccca aattatgcca gacaaaaaca
      421 cattaatcat tttgtacact acctgaaccg cgtatagcca acatcatggc tgacatcaaa
      481 accgctggat cgcgtttcca taacccgcat acccggaggc gaattcacaa aagatacctt
      541 gatctcacca tcaacttttg gcacagaagc tttgttaatc atgaagggat tcgagcctgt
      601 ggcatcggag gcgttgtttg actgagccgg atccaccgcc ggataaggtg tgtatccccg
      661 cgccggtatt cccgtcccat aagcatcata agcacccgcg ccccactgcg cagagttaat
      721 ggcatcgacc gtgtcaccgg aactgtcggt aaaccactca ataattggct tcagcttgtc
      781 ccacatatcc tgaaaccact taacaaccgg tccccagtta ttgatcacca tccccagcgg
      841 cgaccaggca aaaaccttct tcagaagttc ccaacctgcc tcaaaataag gaccaatggt
      901 ttcccagagc ttcttgaaat aaggtccgac aacatcccag ttagtgataa ttaatcccgc
      961 agccagagca atcgccgtcg caatcatgcc aatcggcgtc atcgacataa tcctgctgac
     1021 aatactgatg gcactgccca cgcccatcaa tcccagtttc agaatcgcaa gaccggcagc
     1081 aagcccgacg acgccgcgaa taacccgggg attttcatcc gcaaacttcg tgaatttctc
     1141 ccccaactcc cccagccatt gtgtgatatt tttagcgtca ccagaaaatg cgccgccaat
     1201 agccgcaagg ccgttagttg cggtccctgt cattgcctcc cacaggttgg acagcgtacc
     1261 aagctgtgcc tgaacacgtt tattcaggct ggcctgttta ttcatcttct gctggatctg
     1321 atcgtagcca tcctttcctt tatcgattag tgcattgacc acctgaaggg tttcggcatc
     1381 atcaccaaat attgccttaa gtacacctgt tcgcttaacg tcggtcagtt ttcgcagctt
     1441 tgccagttgc ctgaacatgt tatcaagacc gccaaaactt cctttgccgt cagtaaaatc
     1501 gagctgtacc ccgagtttct ggcgggccat aactttatta acgtccctga ttttcttaac
     1561 gcttaatccg gactggataa cttttcgcag ggcattacct gccgactccc cgttcatccc
     1621 catctgatcc atcatgacgc tgatgggggc aaggctctgc gcagcctgaa gaccatcctt
     1681 gttcaccatc ttcagaacag aactggtttt agtgaagaag gacaacatgt tggtatcgtc
     1741 aacgcccaga taaaacgcct tctggatagt gtcgaacagc cccatcatgt cttctgacgc
     1801 cgttccggta gcatcctgca tctttgcagc aaactcagca gccgcttccg gtgttttttt
     1861 cagttgtacc gcaagataag ctgtcgcttt acccacacca cccagaatgt tttctgccgg
     1921 gatcccctga cgcaccagca tctgcatcat gttctggaaa tcagccgttg taccaggtag
     1981 ctggttaccc aggccaatag ccagtttatt gatgtcctga aagctctttc caacctcgcc
     2041 gttcgcatcc atcatggcga ctttcagccc ggtggcggcg ttttcctgat cggcataaga
     2101 tttcagggaa agcgtcagac ccgctgccag tccgccacca agcgccagcc caccctgtga
     2161 cgcttcttcc gcctggcgtt taaatccccg gattttcttt tgcattttcg acagcgcggg
     2221 agaaagcctg tcgacaccgg tgatcaacgc cttaagctca aattcagcca tgtgtgcgtt
     2281 tctcctgctc tatcctgttt gcctgactga ccagtaaggg aatttcactg atcggcatat
     2341 tcagcaattc gaaaggatta atgcgccagt agctggcgca gtcaaagaag cgatcagtga
     2401 ggtattcagc cgtcaggcct ggaggaaaaa accagccaca agccacgccg ctgcattcag
     2461 gtctgccgga gacatctggt cgacagagct ttgcggcact ttcgccagcc gcacaatgta
     2521 tttcgacacc acatgcgcca gaagtttgac tgactcatcc tgattcatct ggtagggata
     2581 ccccagctcg cggacatcct tcccggtggg ctcatcaaac tccagtacgg agagtgtctc
     2641 gccatgagcg gtaatcggtt tctttaactc aagctctttc attactggta atccccttct
     2701 tcaccgtgga actcaagatc aaccgtgcct tcttcggcat tatggttcgc ttcgccgtgc
     2761 agccaggcgg acgacaatac atagacctga ccgttcgcca gctcggcagt gatggtcatc
     2821 tcatcagacg aggtgatttt gctcaccgga aaattcttcg gcaccttgaa ggtccctttg
     2881 acataaggcg cacggtgagt ttccttgcgg tccactgaac cgtccaggct gatgatgtca
     2941 tcattgaccg tcctgttcat gggcacctca atgccgccgg tcagcgatag ctgctgaccg
     3001 tcaattttga aataacaggt tcccccgata cgggccatta tgcagactcc tctgaatact
     3061 gaagacggaa ctggttaacc acggcaaaga cacgcaactg gttaacatag tcaggcggga
     3121 acagcgtgtt caggcggttc ggatcgctgg catcacgctc cacaaccagg tactgcttaa
     3181 acagttcgta gttttccacg atccccgcac gctcaagctg acggtaggtt gccagcagtt
     3241 cccctttgat caccgccggt gtgacaatcg cctgaccggg accaaagcgg gtaccgtcgc
     3301 tggcaagctt gtgacgcccg tacttactgg taatgacgga tttcagtttg cgcagcacat
     3361 acgcgctggt atgcagcgtc tcgctgtcga ggtagctgtt atccgcaaca ccgtaagcgt
     3421 ttttcctgta cgtggtgaca tcacgctgaa tgcgcagtac cccgctttcg acatacgccg
     3481 ttgccacgcc atgagacagc agggtctgtt gttcggtcat cgtgaaccgt ttccccttcg
     3541 gcgcaggcag catacccacc agctcaccgg tctgcgtggg acgtgccgga tcgttgcgaa
     3601 taaacaccgc tgcgcgggcg gtacggcttg ccgccagctc gtcggcaggc gtctgggtct
     3661 ctttttcgta tcccgccagg gtaatgtgct gctggttaaa ctggtcacct gcggtcacca
     3721 gttctgacag cgtgccgatc tttgccgtat acacatgacc atacagctga cgcgcatagc
     3781 tccagcgacc gctggtatcg ttcatctcgg tcaccagcgt gttaacggag gccgtgtcgt
     3841 tgaacggcag gccgatataa tcaaacggct catccgccat tgcagccacc gcgccggtga
     3901 gaacaggagc gcccgttccg gcggtacccg tcgccacggc aatctgtacg ccagctggca
     3961 gcacttcgcc cccaccaaag ccgtagtaat tgaggctgac aggaatttca ttcccgcaaa
     4021 accccttatg acgcgcggtc agtgtgacca cgcctgccga agatgaggca gtaaacggca
     4081 gggtcggaac ggcattgatg gcatcctgga tactgctggc aatcgtcgcg acgttatcgc
     4141 cgttggtcac cggtgcctgc acgcgggtac gtcccacata aacattcacc gtgccggttt
     4201 cggttgccgc gccggtcacc gtcagcgtaa ctgttgccgc cgcgcccgtg gattcaggaa
     4261 cggcaatcac atacagttca ccaaacgggt cggtctggcg ataagcctcg accatacgcg
     4321 ccagctgact tcccgcacca caaatctggc gtgcatagtc tgccgatggc atcagcacca
     4381 gactgttggc aacaatctct gcaccgttat tggcatgacc aatcagcagc gatgctccgc
     4441 tgtcctgtgc agtattcgcc gccgagttat ccatttccgc ataaaaaatc ggaaccagcg
     4501 tattcgacgg aatggtgtta aagcttatcg tcatcggtat tcaccttttc attcacgcgc
     4561 cggatatcac ccgctgcttc acggcgcagc cagtagttgt tctcatcaac atttcgcccc
     4621 tcggcgggca aaaggtcgcc gcgggcaggg tcaggaactg accgcccttt aacaggtttc
     4681 acaaacatga agattctcag gaaggaaggg ttatttcggt gtgatgttcg atatcgccgt
     4741 caggcccgtt accgggatcg agataatcaa catcaatcgc cagcgttcgc agttcatcca
     4801 gactgttcag gtcatcctgc tggcgggtat cgtcttcggt cagttcgctg atgaccgaaa
     4861 aatcgaactg ataaatcagc tcatgacgat tcagatccag cagcgtgccg ccgtcatagg
     4921 taatcgggtt accgcacgct tccgggttcc agcccagcag agccttaaag agcatctgcc
     4981 ggacatcgtc caccacatca tacgaggcaa actgaccgcg ctcatcacgc ccgttactca
     5041 gtatgacaac cacggagaag ccctctttca gctcctgcca gtagtcggtc tggcttttgt
     5101 tttctcccgg agagtcatca cccggtacca catacgccgc cgggagtctc agctttccga
     5161 cctccggcag atttttgaac tgtgccgcgc ctgccacccg gttttcaaaa tacgggcagc
     5221 gggcacgcag cgcagcaata acaggcgtca gtttcatctg tgtcgtcgct ccggcttcag
     5281 tgatttacgc aattcccgcg ccagaaaata gcgtgtccag ctgcggttct tttcaagcgt
     5341 ttccaccata aagttattac gtggagccag ccgccagccg ctgccaccgg atgcaccacg
     5401 atgatgacta cgacgacgtt ttgctcctcc ccggacacca aaaaacagaa acgccggata
     5461 gaagtcacca gagatcatcc ggttcccctt cccgttgcgc tggttagggg caatgcgtgt
     5521 cataaaaccg gctcgctttt tactagctct cggcaccatg taaccaatcg aacgagccag
     5581 gcgtccggtc tgataaccgg ggttttcacc cggtgccgac cgcgcacggc gcatcaccag
     5641 ccgacgggca tcacgcatat gacgctgccc aatcgtgaca aacgcccgcc ggacacgggc
     5701 gcggttaaag cgcatctccg cgggctgctg aacatcaacg tgaaaaaagg gagtcgccat
     5761 tgctgcctcc gtgactctgc gtaaattcgc ccagttccgt acactccagc agcagaaagc
     5821 gccgcgcccc gttcagatcg cgctgacgtt tcacccggta cacactgtca tcacagacca
     5881 cctcataatc agcagtgatc ccccggcggt agcgaatggt gatgtaatgg gtgatggcgt
     5941 ctccggtctg cgcggtttcc tgccaggtgg tggcactggt ctggataacc ttcgcccatg
     6001 cccggaacgc aaccgggtat tgaggctcca cgccaaagtt atccgcgggc atatccaccc
     6061 gctggcggat caggacgcgt ttattcagtt cgccggggtc cggcagaatg taggttgcgc
     6121 tggtctgcgc ctgacgaatt ttcatagtgg tataaggcga taaggaacaa ccaaccagtt
     6181 aaaactcatt ggcaactcca ttttctcaac gtctgtaacc gttgagcggt tttcgtagaa
     6241 atggctgaca agtagcagaa gcgccagctt cacatcatca gatatcacaa gcccatcagg
     6301 atcatccgca ggcctgtcat ctgcggttgc atacaactta cggttaagga agttttccgt
     6361 acgactctga gcggccttac caagcagttc aagcaactca tcttcatcag agaaatcatc
     6421 atccagacgg agctgaagct taatctcttc catttttaac agcataaaac ctcctgtgcc
     6481 cgccagaacg cgggcacaaa aaaaccgcat tacgcggcgt gctgtattac gtaaaaagac
     6541 taatcaacca ccaacgctac ctttccccac cagcgcttta atggcagagg tgtcttccag
     6601 gatacagtca aaacgatgga aggccagaaa accagtctga tcatattccg cgtaacgctc
     6661 aaccagacgt ttaagaatca tgtatcgcac acgacggata atgaagcgat caaagtcacc
     6721 acagaacatg aattttttac ccgccccgat atcatcaatt tcctgatcaa tgacatacgg
     6781 tacattcaac actgaagcag gtgccacacc aacaatatcc ggcaaccata aagggcgtcc
     6841 ctgaccgtct tccatctcac tgatcagttt cagcgtatta tcgttaaacg ccaggcggaa
     6901 tttcggcccg cgacgatatg caggatcaat gctgtgtttc agagccagaa tttcctgcca
     6961 cttcaccgta tttgccgcgg cagtctgtgt tgtgccggtc acagatgcga ccagcccttt
     7021 gggttgttta ggcgtaccag caccagttcc ctgaatcaga taacgggctt caccacgacc
     7081 aatacgttca gcaatgcgac gggcaagata agcttccata tcgatcgcgc tgtcctgcag
     7141 caactcatta gacacacgaa tgattttcga tgtcattttg agcgccccaa gacttcccat
     7201 accgaaatcg gtgtcttctt caccggcttc ttcattttcg cccagcagaa caccaacttc
     7261 ggaagtacca tcagctgttg cccactccat agtgcgaccg tcagaagtgg tcagaatctg
     7321 cgccacactg gcgatgccac cgtaggattt catcttctca acaactttcg ccaggaatgt
     7381 ttctggtacg gtatatccgc ccttttcatc ctgagctaca ccctgggcac gaagttcacg
     7441 caacgccttt cgttcttctg atgtcagctc actggcaccg tgacgcatcc acttatcaaa
     7501 aacctgagct cgtttctcat cctgttgcgg attgttttcc ggatcaagat tctgacgctg
     7561 ctcttcctca ttgctttcaa tgtacgcctg atcctgacga cgcagttctt cttcgcgtgc
     7621 aattcgttca tcaagcgctt ccagttcgga ttttgctttg ttccactcag tgcgctgctc
     7681 ttccgtccat gcgttatcac caattttttc attcagggcg cgcatgtcag ttgcgatagt
     7741 attacgtttc tgtttcagtt catgcagttt catgatgttt cctttacgcg ttaagaaggg
     7801 tcaggacgcg ttcacgcgcc atacgttgat taatggcttt ctgtagcgcg ccactgttgc
     7861 gcgcctcctg ccatgctttc atggagcgaa cagccgagtc agcctcctga taggcaggat
     7921 atgtcacagg actgacatcc agcagacggg aaaagcgggt tatctcgcga ataacaaccc
     7981 cgtcctcatc ctgataccac tcctcaccgt cacgggcgac acggaaagcg aaagatgact
     8041 ggttaatatc tccacgttgc atcggggcca gcaccagatc acgaatggtc tgtgtctccg
     8101 gagcctggat gtcatagcgt aatccgcgct catcaactga aagattcagc gtgcctgctg
     8161 cactacgccc aagaataaaa ttaggatcgt ggttaaacag tgcgcgtaca tcatcaccaa
     8221 gcacatcgtc aaaagcgccg ggccggatga tttcgcggaa tgaaccgaat atcagctcag
     8281 aacgacagtc aaacaccgat ccataaccga taatgtgcgc cgggttatcg tcatgccgct
     8341 cagcacgcac ctcaccgctg taacaacgga tttcacggtc attcattggt ttttccctca
     8401 tcgttttttg ggggcttaaa atctcctgcc gggttagcag cattcacgct taccagcatc
     8461 tcgtccagcc cttcaaccgg attcatatcc tcaaatgcgc gggcctcatt acggctcatc
     8521 catccatcgg taatagcgaa gtgatagaat tgcgcgcgct cctgcggagt tccgcgtaaa
     8581 agccccgtca gattgaacct gacgtaatac ccggcggcta actcagcgcg ggtaaacaag
     8641 cgacggttaa gctcctgctc ccagttcgtc acccacggca tcatcgtgta gcggacaaac
     8701 tgaatcgcct gcgcagaaat attggagaag gtggcttttt cgaggtcatt aatcatgtgc
     8761 gcaggaatat tgaaaatacc ggcgatcatt gaacggttca gcttcatcat gtcaatgatc
     8821 tgagcgtcaa ctggcgacac agtcagtgcc ttgtaatcca gatcggctgg cagcagcatg
     8881 gttttgtttt cctggcggcg taacgcctgc gatgccttct gccactgttc tttaagccag
     8941 ccccagcttt ccttattgag tccgctttta acggatacta tccccgccgg acgggcatta
     9001 ccgctgaaga agctttctgt gtacttctga ccgctcatcc ccatgcctat tgtttcggca
     9061 tgttgcataa tcggactcag ccccatcttc tgattattac ccagcgctcg gatgtggatc
     9121 atatcgtcgg ggctgatcgc aaacgctcca tattcgttgt acaaaccgta ggtgtatcgg
     9181 ccaccagtat tcatcagcgt cgtttcccac ggcatacagc aatccaggga tatgacttca
     9241 ccgcgacgat tacgtttcac ccaggtatac ccattccccc agccaaggat gtgacgttgc
     9301 ttcagttcgc gccatttgta actggtttgc caggtattgg gctcatcatg aaccagataa
     9361 aacgccggat gatcgcgtgc gggctcaacc ttcccattgt gcctgcgcat aacatgcaac
     9421 ggcatctggg caaggctgga agacaggaca tagatacagg aatacaccgc agccagtttc
     9481 atcgcagttt caggactgac ataaacgtct gcccggaaca gcccatcagt atcaacggca
     9541 tccccggtta tcggggtgga aggattctcc agtgatttac ttctgaacag agcatcaagc
     9601 agcacgcgtc ccccttctga ccataaccag tgctcccacc agcagtaaag caccggacaa
     9661 aatcagagcc ggagccatac caaactgcag gtaaactccg cacgtaagca ggccaaaacc
     9721 agtcagcccg ataacatcag caattagtga tttcatagaa ttaagagatc atcgtccgga
     9781 tcaagagatg agaggaaatc gtcaggttct ttgagcattg cccgaccgat cgtcataatc
     9841 agtgcaaccg caccatcgat tttgttttcc gcctgctcct tgacgggctt cactaaatca
     9901 tcgttaccag gcatgttttt gccgaccaca ttgccgatac accaggtcat gatgggattg
     9961 ccgtcatgat gaaagcgtcc cgattcaatc gctgcttcca gctctttcat cggatcggac
    10021 atattggcga agttctgtac gatagtgacg ggattcagat cttcatcagc aaggtcatgt
    10081 gacagcccgg tcgccccgaa ggggtcgatg ggtgactcac tgaccgggct gattttgttc
    10141 gccgctttgg cctcttcgag gatgtagcga taatccacct ctgcaccatc ggtaacggtc
    10201 aggacgccca tttccaccca tttctgaaag cgttcggctg tccgtctatc ttcatttttc
    10261 tcgacgctgt acaccgtgtc atacggtacc cagaagcgcg gggccacact gtagtaatgc
    10321 gttttaccgt caatctcgcg ggtataaagt cgcgccatgc tgttcatatc cagcttacgc
    10381 gccaggtcaa aggccagaat gcacggctgc ccctcgaact gctcaagggt cagtgattta
    10441 tcctcgcagc tctgccagct caccaggttg aaatacgccg aacgtgccga cacccagata
    10501 ttgaggtgtt ttgttttaaa gacgtttgcc agacgggcgt tatttttcgc acgctgctgc
    10561 tgacttaaca aaaattcgcg ataaaccgac acgccaatat ttggattggc tttttccagc
    10621 acctgcgggt cggtccagtc gtcaccttca tcaacggtat agatgatccc gagcagttca
    10681 tcgttaggca ccgagccgtt gagcatctcg atgacttccc gccgcttgtc gtagcacggc
    10741 ccctcaatgt tgtacccggc ggtggtgatg gcccacatca gtggctgacg tcgcgccccc
    10801 atcccggtaa gcattgtggt ataaagcgca tcggtggcat gctcgtgata ttcatcaacc
    10861 acggcacagt ggggtgatga accatcaccg gggttgccga tcagcggttc aaaccgcgcg
    10921 ccatcctccg gacggttcat gtttgaggcg ttaacctcaa tcccgaacgc ttccgtcagc
    10981 atgggtgtgc gtttacacat cagtcgcgcc gggcgaaaga cttcccacgc ctgtttctct
    11041 gtcgtggcac cggaatacac ttccgcgcca aactcgttat cacaggcaaa acaatacagg
    11101 gcaacaccgg cagagattgc tgatttgccg ttcttacggg ggatttcggt atacacctcc
    11161 cggaagcggc gcaaccgggt gcctttattg acccagccaa acgcacagca gatcacaaat
    11221 agctgccacg gctccagcgt gatgggcatc cgtttgaatg cccactcacc cttggtgtgc
    11281 ggcaacagct gaataaattt cgcggcccgt tcagccaggt ccttgtcgaa gcggtaacga
    11341 aacgacttac ttttttccgc catcaggtca tcaagatggc gctggcaggc ctgaatcaca
    11401 aactggcagg ccacaatctt tccgcgcacg acatcacggg catactgatt ggcagcattt
    11461 acgttggggt aagatttccg gctcatgatt cgataatttt cagaaacggg ttagtggctt
    11521 tcttctgccc cgccaggcca atcagacgct ggcggctgct ggggtcgagt ccgagcattg
    11581 cccccgtgct gctcatctcg gactcctgtt cttttttggc ggtcagctcc ggatttttga
    11641 ccctgccgcc cattgcaccg gtgatggtgt tgccctgtat ggcaatattt ttcacggcac
    11701 gtcgccagaa ctcataggcc acgcaccacc gctcaagcac tgcgaggtca gtcacgcaca
    11761 gcaggccctg accgcagagt tctttggttg tcagttgcca catgatcgtg gcgagaggga
    11821 gatcttcttc agcgaaccac tctggtggct caacaccttt gatgggcgta aaaacaggtt
    11881 catctttgtt cagggctcgc ttgccggggt ttccggccag cgccttgcgc gccgttggct
    11941 tggggcgacg cccggaacgc cccgccgttc cagccatatg cggcactcct ggttaaattt
    12001 catttttcgc gggtataaaa aaacgatggg gcgggcagtc cggaagacgt caggctgcag
    12061 ggatttgacc cgcccctccc ctcaggcagt tgagaattat tatcacttta accgttcacg
    12121 ggccgtcttc gccttatgac acggccagca cagactctgc agattactgt cagcatcagt
    12181 gccgccatgc gctttaggga tgatgtggtc aacagttttc gcctcacgca ccacaccagc
    12241 acgcagacat aactgacaca gacctttgtc acgcttcaga acacgcgcgc ggatactgtc
    12301 ccacttcgaa ccgtagccgc gctgatgacg ggattgtcca ggtttgtatt gcttccagcc
    12361 ttcgcttttg tggctttcgc agtagcctga agggtcagta gtggtatggc ggcagccgcg
    12421 aacacggcag gctttcgggg ttcgtggcgg cattaatgct tccctttaag ttattacgat
    12481 ggaacagacc atagaaatgg caataaaaaa ccgcccggag gcggttcaat tatcatctcg
    12541 ataaactaaa tcagatcacc aatgtatttt gcactaattg aaatttgcat ctgaggcatt
    12601 ccgaccacag atccatttaa caggtaatca cgtcctcgtt cctgcaaaga aagactcaat
    12661 tcaaagttct ttaccccagg gaaaaccgag gtggcattta aatcatgctg cgatacgcgc
    12721 agaataagtt ggctaccgtc aacttttccc tgatacgtaa aaccaaaatc tccgccgttt
    12781 actgcattgt ttttgacaac tacggtacca ttaccaaaat cacgctgatt gcttctgaaa
    12841 acaacaaaat agataccatc tttcatgtgt aaagcccttt aaaagagtca ccaaaatcag
    12901 gtgctttgta tctattgggc catcacatat caaatcaagg aacaaaacaa agttaacatc
    12961 attttttttg catgatgtga ccacgctcaa cttcaatcct tctgatgtca gctttatcgg
    13021 tattacattg cgccaatgca gacaacaagg cgacattcag atctaagctc gagccccacg
    13081 taaaatgatt aggtaaatca ggctgagggg tttcagccgt caggctggct ggtaacggaa
    13141 ctaccggaac ctggacgtaa actgttcgcg tacttccgca accggtcagc agcggcagca
    13201 ggcacaggac gtgaagcaca atcatcatcc gcaacagcca ctttgatatc tttctgggtt
    13261 ctctgtgact ccagtgcgat ctgctgtttt gcatgttgat tcgcctcctg aatgatgttc
    13321 gttattgcca tagtacgcag aacattcgcg gtgatagcct cagtagaatc agctcgctgt
    13381 tccgcagcat cagcacgctt ctgctcctcc agaaactttc catgatagtg attcgctgac
    13441 cagacaagac caccagcgac acaagcaata aacgttaaaa tgagcgccca ataactcatc
    13501 ttcataccag cagcgccgcc cgcgccttgt tgtatcggac cttacgatcc tcaataccgt
    13561 tcaaaccgcc gttaatgatg cgcgtaacac ggttaatatc ggcaccgtag atcatgcaac
    13621 ctttagaggt gtagaaccat gcagctgagc gcgcagcctg tagttcctgt tccagttgtt
    13681 caggtgaagt caccagatct aacttcagcg ccgcgccaca gatgcgataa ttatggaggc
    13741 cagtgatttg aattaatcct ctaccgcgat atttccagcc atcaccgggt gctttgttac
    13801 ccagccggtt gctatacacc agattggcaa tagcatcctg gcgagctgca tgtccggatg
    13861 ttctgccaag ggcatcagcc tgctgctgtg tgatcctctt tccgaacgtc gccaccagcg
    13921 cagatggcgt gtagttaaaa ttttcaacta cggcgctaaa ccccatcgac tcatggccta
    13981 cctgagcgat aaacattgcc tgatccgctg gtgctgtaat gccgaattcc ttcatcgccg
    14041 catcaatgtg cggaaaccag cgcgcagcca gcccggcgct aataccagcc gccttttgaa
    14101 ataattgttg gttcattagt gcctcagatg atcaaccaga cgtgcaacgt tgcctctgac
    14161 agccaccagc acggaaagaa aaatagtgtt cgccacgata atgggccatg aggaatgggg
    14221 ataaatccca cagagatagg ccaacggaac agcactgtat gtaacagtaa tcagccaggc
    14281 taaacgtgaa acccaaggac gatgccgcga atcaccacga cgataaaaca tcagagtaat
    14341 aacaacacaa gcacataaca gcgcatttat agttgctgtc gggtcattta gctccacctg
    14401 aacctccccg gcgcgttatg agcgccacca gcgagccgat atcctgatta ttcaggaacg
    14461 tcaggatttt aacggctaaa gcagagacga ttacggcacc aatagcatcc agaggtttat
    14521 cactgtatcc ggtcaagttc gccagcttgg agccaaccaa cccagagcaa agaatcccgg
    14581 caatatatga cacgataaaa tatgccagtc ggcgcgatgc actcagatct gcagctgttg
    14641 ctatgtagaa tacagcccct gcaaatgcgc caaatacaac gccgtaatca gttccggtca
    14701 gcagtccata aacactggca cccgtcaggg caccaccagc cagcccagta ccggaaatcg
    14761 gatcggacat ttagccccct cttaattgct gttggtcctc tcagaacgag gggaaacaaa
    14821 aaaggccgca ttaaggcggc cttggtgagg tatagttttt ttaagataaa ctttgattga
    14881 tatactcatg acacccaaaa ataaaggcgt tttgtgcatc aataaatgac tttttttctt
    14941 ccggagtaaa ataccctttt gtaaaacata cttttagcgc tttttgaaga tctgccgtca
    15001 gggttatcaa cttacaacct tcctcatcag gccttattaa tagaagtaat ttatttttac
    15061 ttatttcagc ggcctttatt ggaacttgaa tctcctcagg gaaaacaccc ttagccaata
    15121 tatccatatt cttatttccg accagatgcc attgctcaaa tgtgctggcc aacataatta
    15181 cgtctgtcac atgctcagcg ccagcaaaac gtatttgttg cgccaattct ttattagtat
    15241 tcaagtgagc ctgtaatgtg gctaaggcaa aattttcttt gattgccctg tatgcaaccc
    15301 aaccagttat tcctgcggca ataacgcctg caagagcagt aatgagtgtt tcaaaaggaa
    15361 aggaagaact gatttcaatt ggcggtaatt tttctattac caaggtcaac tcgccagtag
    15421 ttttttcgaa agcgtacggt acgttttgcc aagtcatgaa gcctcctgag tttcagagga
    15481 atcataacaa aaaaacccgc tcaatggcgg gctcttaatg ttgtgttgct cagttcgctt
    15541 taacgtcccg agcctaccac aatttaagca ctttcttgct cactctgcaa cttaaatctg
    15601 tcgctatttg tgccgaatgc gtcacaaact ggagcgtaca ggatcgattc tgcaagactt
    15661 agccaagtgt caatgcgtcg acgacaggtg atcagcggcc agtcaggatg tttagcctgc
    15721 agttcattgg ccatctgcaa cttgctctta cgaagacgat accggtcaac aatcacgcta
    15781 tagagcgatc ggtagtcatc attcatcagt actgaagcaa tgacaccgtc cactaacaac
    15841 ccttcttcat ctgaacagaa cgccaggccg cttttatttt tgctgttgag aatttcacgc
    15901 aggtacgctt caagttcagg cttggtgata cccgctatct tcatccggcg tagcgcttca
    15961 ttgatagctg tcttggtgat tttcccggat gccagaagct ggttaaacat gtttccacca
    16021 ctaccaccgc cgatataaga ccagcggccc cacatgcgca acttgccttg tatccagata
    16081 ctttccagag tacgaaggcg aaccatttca cctgatttgc caacttcaga aggattaatc
    16141 atttagcatt ctccacttac gccagtacgc ctattgccag cgcacgatcg ataaaacgaa
    16201 atatcagctc cagctgggag ccatacttct cttcaaatgc cacggtatcc gcatgcagct
    16261 cgtcgtgatg ctttctgcac aaaggcaaca caaagaggtc atgcgctttt gtacccattc
    16321 caccctgacc gtgacctatc aggtggtggg gatcatcagc aggctttcca caacatgcac
    16381 acggctgtgt cttaacccag cgcgtgtact tttcattaac ccagcggcga cgttttgggc
    16441 gtaacataaa agactccggc gactccggat ccactttcag cgccagcacc ttttttgcct
    16501 tatcctggat gatgctggtg gcaggaaccg aaggcacaag gtcactttcc cgggtgacag
    16561 acggcacaac aggcttcggt aatctcagtg ccttacgggc tgcactttcc ggtaaggcac
    16621 ccgccaggtc attacgaatc agccaccagc acagttccgg cattgtcaca gcgtgactat
    16681 catcaaaacc gagatcccga cgcacaacag acaacaccca gcgggcacag ttatccgttg
    16741 ccattgattc cagccgttcc gtgaactgat cgcgcagctg gttatcgcag tgccagcaca
    16801 gacggattgc acccggcgcg tgtcgcattg tggtcatgtt ctcgctgtgc cagtcggaat
    16861 gaggccactg gcagcctttt tcacgaagta accagctttc aagacattcc acgccaccag
    16921 cacgacggat cactgcctca ttgcggaaca cggcccgaac ggcaggatca tccgccagcg
    16981 gttgtgatgc cgccggaaca gcaccactgg cgaaagatga ataacgttcc ggctcaggct
    17041 ccagcaggac acgcccctgc ataaacaggg gcatcagctc tgaacctggt ctgaacaata
    17101 cgatccccat acgcggggca atttcagggg tcagtagtgc tctcacggtc acctcaatga
    17161 acgatatcga gcagctttaa cagctcaggg aatcgggatt cgaagaaatg cggctgcgtc
    17221 tcgcgcggat ttgcgggact ggtgatgttc ttgccgaaca tgcagccttt cgctgtcagc
    17281 gaccagaatt ttttgatgtt gttaatcgcg gtacggctgt atcgttcgcg ctgctcgacg
    17341 atccccagct tcaccatctg gtgatatgcc tgattagccg tcaggcgtat accatactgt
    17401 ttcagcagtg cactcagtga cagtgtcggg cgacttgagc catcgtgtgc atcagcagga
    17461 gcatcaatgg catagcgcgg tgccagattc ggtaagccaa cagcctcctg gagtttctga
    17521 caggcaccaa gcactgaaga gttagacagg tttaattccc ggcgcataaa gtccagcaga
    17581 atcacaccag cctgcatctt gtcagcagcc tgtccggata atttttccgg tgcgctggtt
    17641 accatatcga aagtacggat caccttcaga tggaatgacg ggctgatcca cattgcatag
    17701 gcatacacca gttctttgca gacatacgtc ccctggttat ttccgccacg aataacgtta
    17761 actggctcta tattgaccga gttgcaaatc tgcaactcgc ttattaaacg ttcagtttgc
    17821 tcattgcgga gccagaatgc aggcttatgc ttatccagag aaccggcagc cctgtgcaga
    17881 tcgttcaggc tgtaacgccc ataagcatca cgacgaactt caataccatc aatgaccatc
    17941 agattattca tacttcgttt ctcctcttga tcaggcggct gcacccgccg ttttctcgta
    18001 cttactgata gtgatctcga tcttccctcc cgggataacc ggtccccact caaccagcat
    18061 tcttttcacc tgactgtcgt cttcccacac acccgcgtgg gtcagggcgt caaacagcgc
    18121 cttgttatag ttgtccagat cgcggatccg gttatccgga ggaaacaaca cgatctccac
    18181 tgaagcaggt gccgacgttg gtttcggcag gcgacgtaac tgctcaacta ttgctgcgca
    18241 cgccgcgctc tggaatttgc gccccgctgc gcttatcaga ctcttaccag caaacgcccc
    18301 tttgttgggg tgtcgccagt acgtgttcac gctgggcggg aaaggcaaga tcagcttcat
    18361 actttcaggc ccctctcatg taaccagtgg gctgcacgca gcctgccgtt ttcctcaccg
    18421 gcaagcagtg cgcggataat cccgaccgcc tcgctgtcgt cgtccttcac cgcggtatga
    18481 agcgttatcc cccgggccac gccacgcttt atcgtgatga cgcctttttt ctccagtgca
    18541 cgaagatgct ctaccgctgc attcactgaa cggtatccca gcatggttgc cacctcctga
    18601 ttggttggcg gaaagccacg ctctttctga taagaaatca gcatatccag cacctgctgc
    18661 tggcattgag ttaacgtcgt catgccgcca tctccctgac cagtttttcc gcctgctggc
    18721 gaacctgcgc cagaaacgcc tcaccacata cctcaagttc atcgcgcccg atgtagctga
    18781 ttgccggtcc cttccaggtc ttatcgaaaa cagcaatagc accagcgaag aaagcgcctg
    18841 tcggcacctg cttctcatcc ttcgggataa accaggcagg cagttcaaaa ccaatacgcc
    18901 cgcgaataaa agcaatatgg tctgcatctt ccggccacca cacttcgctg gtggcagctt
    18961 tgatcaggaa aacatagcgc ccgcctttat cacgcatagc actggcatgt ttcatgatgt
    19021 aacgcatgcc ggtgatgtat tgcccctcat gctgactggc gcggctgtac gggggattac
    19081 caaaggcagc acctttaagc tccgcaagac gttctgacca gtcatgcgcc agcgcgttgt
    19141 cttccgcagt gtaataagcg gcacatttgg cgttatcacc atcagtaaac agatccagaa
    19201 caaacgggcc aaacagggtg ttaattcccc agaaaatgtt gtccggcgtg cgccactgat
    19261 cgcccacttc cttcagttca tgggctggtt tgttccgcag ctccaccagc gcctggcaat
    19321 atttattact cattaagccc ccacgtaatt ccctgacaga taccactctt cacccgatgc
    19381 agcgcgcttg ctgcttttcc gtaagcaccg ctcacgatgc gccagaaaat tgtttcgttc
    19441 tggctgggag tggctttcac ggaatgccgc catccacacc gttgcagctc gacggaataa
    19501 gcccctggac tccagttctt cagcctggcg ggtcaggcac aaaatcaccc gggggtcgtt
    19561 agtgccgaca tagaaattgc gcacaggtct ggtttcacga acagattgtg gttccggctc
    19621 ctgcgctctc tcagtcaggc gcgggaaatg tctgcgtgta tctccttcac aacggtgagc
    19681 cacacgccca ctctgacgta acttgcttgc tgactgcaga acgcgctgcc gtgagtaacc
    19741 agcaaaagca tccgcaatgt ctccggaagt acacccagga tgggcttcaa tgaatttctg
    19801 aacgtcattt aacagactca tgatcacccc ctgaatcctg ccgggatctg gctgtagtcc
    19861 acgttgtcgt aactggattt gaagtacggg tcttcgcgtt tttcggtgta cgtgctgacg
    19921 gacggcgata agcgcaggga aagctcatcc catttttccc gcagcttcga cgggctgagc
    19981 acgttacggc accagaacgg atcgcgactg acgcggctgt acatctcgca gatttgttta
    20041 tgattacgac catcctgcac acacatcagg cgaatttcgt ttgcccaggc tgtccagttc
    20101 ggttctttgg gacgaaccac ctcgccgtca cattcggcgg cctgctcgta cagggcgatg
    20161 atttttttcc agagccactg tgcgcaggtc aaatcatcct gcgttcccca ctggcgcttt
    20221 ttagggctga atacaaccgc atcaggatgg cgagttaaaa aatcctgttc agccacctgc
    20281 gtgtccggtt gcgaagcgtc cggacgagaa gaggttttat tctctgtagt aatctctgtt
    20341 gtattctctg taagatcatt gggccatttt gacccgatga cagcgtgtcg ttttgaacca
    20401 atggatcgtg tcattttgcg cccatccatc aggtcacttt gacccgatgg agaagtgcat
    20461 tttgacctga tggattcgtt cactttgacc tcttctaaaa gctcactttc atagttgatc
    20521 gtgtagaaat tggtcatgtc acgcttcgat ttattgagtt gctcgcgacg caaaacccca
    20581 agtgatttca ggcttgcaaa tgtgcgtttc agagtggact ctgaccagaa cggaaactgc
    20641 tccagccact gttctgtcgt gttataaacc cagcgaattc cgccatgctc agtgcctgaa
    20701 ttcgtttcat tcagccagta atgaagctgc tgcaacacaa ttgcctcatt cagaccaata
    20761 cggcatgcaa gatcacgatt tatcacaatg ggctgggatg tcattaacag gctcatgacc
    20821 gacctctatt tccctgaatt tacgacgaaa ctgttcgagc gggctgaagc actcatgctc
    20881 atagccttca cggaggtaga taacccgttg tgtttccggt tcccaacgaa tgactctgac
    20941 gggcactccg tagtgatctt tgaaccagcg gttaacttgt cgcaaaggac tgtctccttc
    21001 tgccggttga aatcacccac agcccactca gcaaagctgt gggttacaat ttccctgtca
    21061 cctggtacat ttactgcata gcaatactcc accttcgctt ttccacccgg tacaggaagt
    21121 gcaatcagtt gcgagcgacg gtagtgtgtt gttaaactgt tcatgcgtta gtttctccac
    21181 aaccagaagc aatcgacgcc acgacgcccg gagctgcaca ctcgcgggcg tcattacttt
    21241 ctgaaatgca aaaaattttg tagacaagtg ctgcatgctc ctgcagcttc gaaattgaga
    21301 gatacagctc gtcgttgatt gctgtcttct catgcggttc caccacaccg tcttcgattg
    21361 ccgaacgaat ctgtttggaa taactgccaa tctgttcaat gacttccagc aggcgctggt
    21421 taatatcggc gttgtccaca tcctcgacgt caggaagaga cacaaagacg ccatttgccg
    21481 actgcgccac agcgtcagca atgaagtgag ttccaccagc acgttgcaaa atcattgccc
    21541 atcccagcgg gaaaatctga tcgccatcgg cacgaaggcg gttaaataat gcgttctctg
    21601 ttacatccag ccagtcagct gcttcagcgt aaccacccgg caactttgcg atagtttttc
    21661 tgacagcttt cacgtaccac tcaggctgtt tttctacttt ccagtgatgc ttacccacgg
    21721 cttacctcct gttcctgtgg tttaaaccca ttctggtttt ggctagattg aaaacgtgcc
    21781 ggataaagaa tctgcatttc gctgatttca cccttaaaaa aattggccag acgctctgca
    21841 agatcgatag atggaatttg ttccagtctc tcaatacgac tcagcgttgc tggattaacc
    21901 tgaacgcccg cagcaacatg ctgcaaagta aacccgtgcg ccttacgcac atttcgtaat
    21961 ggtgattgca tataacctcc acatattgcg tgatgagcat attatttcac gcaaatattt
    22021 tgcgcaagtt gatttgctta acgcgcaata aagaaatgta ataaacgcat gaacatagga
    22081 aaccgagtca gacaacttcg ccaggcgaag aacatgaaaa tcgccgatct cgctgaagca
    22141 ataggagtgg atgcggcgaa tatctcgcgc ctggaaacag gtaagcagaa acaattcact
    22201 gaacaagccc tgagtaatat tgccaggagc ttaggtgttg atattgctga tctctttacc
    22261 tcagacgtca aaagtaatac tgtatgtaaa aacagtatta gtgaggatgt tgcgcaggtg
    22321 aaggatgtat tccgtattga aatgctggat gtcagtgcca gtgcgggaaa tggccttatc
    22381 cagggcggtg atgtcattga tgtgattcat gccattgaat acagaactga taatgctgta
    22441 tcgatgtttg gcggacgacc agccaatcac attaaagtta tcaacgttcg tggggacagt
    22501 atgtgtccaa ccattgagcc aggagatctc atcttcgttg atgtcagtat caatcagttt
    22561 gatggagatg gtatctatgt atttggtttt gatgataaaa tttatgtcaa acgactgcaa
    22621 atgatacctg acaaactact ggtgatatct gataaccaga tttaccgtga atggggaatt
    22681 accagcgaaa atgaacaccg gtttatggtc tttggaaagg tcttaatcag ccagtcacaa
    22741 acccttaaac gacacaatta acccttactt cctcatcaat tagccacccg aaggtggctt
    22801 ttcattaccc atcaaattgc atatctcgca ataaaaacac ttgcataatg cgcaacttca
    22861 ttttatcttt ctttccagac caacaaacaa ggtactaaca aaatttggtt gtaacacggc
    22921 gtatggcaca tgcgtcgtta gcggtctggg gacgttaaag gggacaatcc actccttgct
    22981 cgggcaaaca aaccaggtag ccggaatgtg caagtcaatg atgatgctga taagacgcct
    23041 aaccagcgtg gcgattcggt ttgacgcctg ggaagagacc agggtgcaac gatgagggca
    23101 tttatggaac cgccacaaag tgtggtgccg taactggcta agtgctctca gcgttgtggt
    23161 gaatgcgcag gctgatgcgc gaaagacatt gcagctattg cggaaaagag ctgttcggcg
    23221 gggcaattaa acgcccgtga gagtctgaaa taaccgcaag ccggagatca gcaccggtca
    23281 ccacaacagc cactgctttg gcggtaccag tttgtacact tgcttccggc tggtaccgct
    23341 ctttttacaa aacagagaag agcatcaccg gacgacgggc tcataaccca atccacccgg
    23401 gcggctgcca ccgcaggtgt tcttctctgt tttgtggaga aaccaaccga ccttgcaggg
    23461 ccgatatgat gaggagcagc aaaatggcta gcgaacgcag tactgatgtg caggcattta
    23521 tcggggagct ggacggcggc gtatttgaaa ccaaaatcgg cgcagttctc agtgaagtcg
    23581 cttccggtgt gatgaacacg aaaaccaaag gtaaggtctc gctcaacctg gaaatcgaac
    23641 cgtttgatga gaaccgtgtg aaaatcaaac acaaactctc atatgttcgc ccgactaacc
    23701 gcgggaaaat ttccgaagaa gacaccaccg aaacgccgat gtatgtcaat cgcggtggtc
    23761 gcctgactat tctgcaggaa gaccagggac aattactgac tcttgccggt gaacctgacg
    23821 gaaaactccg cgcagcaggt cattaatatc gttcttaatt aactgattat ttatctcatc
    23881 actgaatatc tttatatagt gaggacttat tatgtctcag aacttagacg caaccgcaat
    23941 taatcaaatc catgccctta tttctgctca gggtgttaat gaaattatca gtaagattgg
    24001 tgccgacgca gtggcattac ctgaaaattt ccgcattcat gatctggaaa aatttaattt
    24061 aaatcgcttc cgtttccgtg gtgcgctttc cactgccagc atcgatgact ttacccgtta
    24121 ttctaaagat cttgcagatg aaggcacccg ctgctttatc gatgccgata atatgcgagc
    24181 cgtcagtgtg cttaacctgg gtactattga tgaaccaggt cacgcagata acaccgccac
    24241 tctcaaactg aaaaagacag caccgttctc tgccctgttg tctgttaacg gcgagcgtaa
    24301 ctcccagaag tcactggcag aatggattga agactgggcc gactaccttg tgggctttga
    24361 tgctaatggt gacaccattc aggcaacaaa agcggctgcg gcagtccgta aaataacgat
    24421 tgaagcaaac cagaccgctg attttgaaga taatgacttc agcggcaaac gctccctgat
    24481 ggaatctgtc gaagcgaaga ccaaagacat tatgccagtg gcatttgaat ttaaatgcgt
    24541 tccgtttgaa ggcctgaaag aacgtccgtt taaattacgc ctcagcatta tcactggcga
    24601 tcgtcctgta ctggttctgc gcattattca gctggaagca gtgcaggaag aaatggctaa
    24661 cgaatttcgt gatctgcttg ttgagaaatt caaagacagc aaagtagaaa cctttattgg
    24721 tacttttacc gcctgatttc cttactgcaa atgcccctgc gggggcattt atggaaacat
    24781 aattgactca ataatcgcct gatggcgagg gttttcttta accaaaattc tgcgcggtgc
    24841 agcgcatata cgtggagaac aaaatgtcat ttattaaaac tttttccggg aagcattttt
    24901 attatgacaa gataaataaa gacgacatcg tgattaacga tatcgcggtt tccctttcaa
    24961 atatctgtcg ctttgcagga catctttcac acttctacag tgtcgcccag catgcagtgc
    25021 tttgcagcca actggtgccg caggaatttg cttttgaagc gttaatgcat gatgcaacag
    25081 aaacgtattg ccaggacatc cccgcaccac tgaaacgact tcttcctgac tataaacgga
    25141 tggaagaaaa aatagacgcc gtaatccgtg agaaatacgg gttacctcct gttatgagca
    25201 cgccagtgaa atatgccgat ctcattatgc tggcaaccga acgccgcgat ctcggacttg
    25261 atgatggctc tttctggcct gtattggaag gtatcccggc aacagagatg ttcaaagtta
    25321 ttccactgtc tccaggtcat gcctacggga tgtttatgga acgttttaac gagttatcgg
    25381 agttacgcaa atgcgcatga atgttttcga aatggaaggg tttcttcgcg ggaaatgtgt
    25441 accgcgagat ctgaaagtga acgaaacaaa tgctgagtac ctagtacgta aattcgacgc
    25501 gcttgaagct aaatgtgcgg cactggaaaa caaaataata ccagtgtcag ctgaattgcc
    25561 acctgcaaat gaaagtgttc tgttatttga tgctaacgga gaaggctggc tgattggctg
    25621 gcgttctctc tggtacacct ggggacaaaa ggaaaccgga gaatggcagt ggacatttca
    25681 ggtcggggat cttgaaaacg tcaatatcac tcactgggca gtaataccaa aggcaccgga
    25741 ggcaggggca taatgaccac atttaccgac aaagaactga ttaaagaaat caaagagcgc
    25801 ataggcagtc tggatgtgcg agacaatatt gagcgccgtg cttatgaaat tgctttggta
    25861 tcgctggaag cagaaccggt ggcatggctg cattcagaca atggcttagg tattccggca
    25921 ataacacgga gtaaaaacat tgctgacagt tggttatcaa agggctggta tgttcagccg
    25981 ctatatatag ccaagccagt gccggtggtg ccagatgctc gtccgtcttt aaataatggc
    26041 atagtcggct ttgatgaagg ctggaacgcc tgccgcgccg ccatgcttaa tggtgccgaa
    26101 cctgtaagcc agacttacaa gttgaacgag ctgtcgggca actctccggt aactccggat
    26161 ggttggataa gctgtagtga gcgaatgccg aacgataaac agtatgtttg gtgttggggg
    26221 aagttttacg gctgggctga gtgcgatacc ttcgaagggt attacgattg gtcgaaaaac
    26281 aaatggtggg cagttactga cgatgtggaa gaatcggcat cgaaagtaac ccactggatg
    26341 ccgctacagg aaccgccgca tgaggtgaag taatgaacaa cttaatgaca actaaacaag
    26401 tcgccgactt ctgtggcgtt tcaatatcaa cagtgcttcg ctggaacagc ataaaccata
    26461 ggacaggtaa gaaatataga ccagactttc ctgatcctga tataaaatcc tgtcccaata
    26521 aatgggcttc acacaagatt tatagatttg caggtattat cgagtaacgt ttattagttt
    26581 cgatgttagc caacacatct atggcacaat gtcaaaccca atcttacttc ctgcgctgta
    26641 ctagaacggc cagccctgac ttcataaatt attaaccaac tgggaacaag ttagctagtt
    26701 ggtgatgggt gtcagttgtc gaaagtcccc ccccaccatc accactagct gagactcttc
    26761 agtgttacaa ttgctttaaa tggttgccaa agtactgaaa tcgagtctaa tagtaaagtt
    26821 tatatctcac aaacagtata tagggacaga gaatgaaaca tttaaatgat ttccccaaaa
    26881 gagatagaaa tcacagaatt gaaagacttg caatatcagc ttttgaacat ttccttagtg
    26941 attcgaatga tttctttatt caaagtaagg atataaatga ttacggcgta gactatcaac
    27001 tcgagatcac aacaaacggt caagcaacta acattagact gtttgttcaa ctaaaaggta
    27061 ctgaacaaga gttaaataat gatggttcgg ttagcatatc tgttaacaga gccaatctca
    27121 actatctatt aatgcaccct tattcgttct atgtgtgttt tcatgcgcca agcgacactc
    27181 taaaaataac attcgcggac agcgtgattc ggagttatga acaatctgaa aaaaaatgga
    27241 ttgagcaaga aactataact gtaagattca ttgataatat atctgagtct cgtttgcacg
    27301 aaattgcaga ggtggctagg cttaatgctt tagataatcg taatgcaagg atagaattta
    27361 taacaaagcc tatttcaaat gttagtgcag ttataaataa aaccataccg aagatacata
    27421 taccagaaga taaaaaacta gcccatgaat tgcttaattc gttatatgaa aataatcatg
    27481 atgatgtaat aagtg
//
LOCUS       DAPGRN010000062        27449 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPGRN010000062 DAPGRN010000000
VERSION     DAPGRN010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.062"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            246..1334
                     /gene="pdeL"
                     /locus_tag="RHW33_002751"
     CDS             246..1334
                     /gene="pdeL"
                     /locus_tag="RHW33_002751"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HDV1646238.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(1376..2308)
                     /locus_tag="RHW33_002752"
     CDS             complement(1376..2308)
                     /locus_tag="RHW33_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1646239.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(2400..2897)
                     /locus_tag="RHW33_002753"
     CDS             complement(2400..2897)
                     /locus_tag="RHW33_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV1646240.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            3155..3760
                     /locus_tag="RHW33_002754"
     CDS             3155..3760
                     /locus_tag="RHW33_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV1646241.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHMTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            3800..4663
                     /locus_tag="RHW33_002755"
     CDS             3800..4663
                     /locus_tag="RHW33_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV1646242.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAE
                     NFHLIAWQQWYDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRQAVIQAVNDKQNI
                     ASAVANMMGLGIGLTPSADDYLTGLVLILFICGHPAEKYKEEFYLGLQRGRKNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            4653..6200
                     /gene="fdrA"
                     /locus_tag="RHW33_002756"
     CDS             4653..6200
                     /gene="fdrA"
                     /locus_tag="RHW33_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV1646243.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            6200..7618
                     /locus_tag="RHW33_002757"
     CDS             6200..7618
                     /locus_tag="RHW33_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV1646244.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPILRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            7668..8102
                     /locus_tag="RHW33_002758"
     CDS             7668..8102
                     /locus_tag="RHW33_002758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646245.1"
                     /translation="MERESVGLISVAHQAFVTIAGCGVNALSGLQVAQEFVGLISVAH
                     QAFVTIAGCGVNALSGLRMAQESVGLISVAHQAFVTIAGCGVNALSGLRVARESVGLI
                     SVAHQAFVTIAGCGVNALSGPRVAREFVGLISVAYQAIWRWS"
     gene            8134..9084
                     /locus_tag="RHW33_002759"
     CDS             8134..9084
                     /locus_tag="RHW33_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV1646246.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPEQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            9094..10476
                     /locus_tag="RHW33_002760"
     CDS             9094..10476
                     /locus_tag="RHW33_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV1646247.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNGKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            10775..11185
                     /locus_tag="RHW33_002761"
     CDS             10775..11185
                     /locus_tag="RHW33_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV1646248.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGIKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            11439..12422
                     /locus_tag="RHW33_002762"
     CDS             11439..12422
                     /locus_tag="RHW33_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1646249.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            12471..13955
                     /locus_tag="RHW33_002763"
     CDS             12471..13955
                     /locus_tag="RHW33_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV1646250.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            13948..14919
                     /locus_tag="RHW33_002764"
     CDS             13948..14919
                     /locus_tag="RHW33_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646251.1"
                     /translation="MAELKKRHEFWLALLIVVMFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            14916..15872
                     /locus_tag="RHW33_002765"
     CDS             14916..15872
                     /locus_tag="RHW33_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646252.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            15959..17008
                     /gene="yahK"
                     /locus_tag="RHW33_002766"
     CDS             15959..17008
                     /gene="yahK"
                     /locus_tag="RHW33_002766"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV1646253.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGCVVAVGDQVEKHALGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            17251..18066
                     /locus_tag="RHW33_002767"
     CDS             17251..18066
                     /locus_tag="RHW33_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646254.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            18479..18724
                     /locus_tag="RHW33_002768"
     CDS             18479..18724
                     /locus_tag="RHW33_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646255.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVIPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(18741..19412)
                     /locus_tag="RHW33_002769"
     CDS             complement(18741..19412)
                     /locus_tag="RHW33_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV1646256.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            19559..19834
                     /locus_tag="RHW33_002770"
     CDS             19559..19834
                     /locus_tag="RHW33_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1646257.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(19934..21520)
                     /gene="prpR"
                     /locus_tag="RHW33_002771"
     CDS             complement(19934..21520)
                     /gene="prpR"
                     /locus_tag="RHW33_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV1646258.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALSKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLLRYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            21759..22649
                     /gene="prpB"
                     /locus_tag="RHW33_002772"
     CDS             21759..22649
                     /gene="prpB"
                     /locus_tag="RHW33_002772"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV1646259.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQAK"
     gene            22805..23974
                     /gene="prpC"
                     /locus_tag="RHW33_002773"
     CDS             22805..23974
                     /gene="prpC"
                     /locus_tag="RHW33_002773"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV1646260.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            24008..25459
                     /gene="prpD"
                     /locus_tag="RHW33_002774"
     CDS             24008..25459
                     /gene="prpD"
                     /locus_tag="RHW33_002774"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV1646261.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDTLREKINCFEDPSFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            25499..27385
                     /gene="prpE"
                     /locus_tag="RHW33_002775"
     CDS             25499..27385
                     /gene="prpE"
                     /locus_tag="RHW33_002775"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV1646262.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         6771 a         6694 c         7394 g         6590 t
ORIGIN      
        1 tatctgaatt ttaatattgc attcttgcgt gattatctcc tgagtttgac ttgtgattac
       61 ctttttaagg tatttagcgt aactgttttt gagcgagcat cagaggtaaa gataatcttc
      121 ttgatagtga tgtgggatgt tatacgtatg gcatcgctaa tgtttatggt taccccttat
      181 gtgtgctcag gaatccacag gtaatcactc atactgaaca gcgataaaag ataaaaggtg
      241 tgttcatgaa ttcatgtgat tttcgtgttt ttctgcaaga gttcggtaca acggttcatt
      301 tgtcattgcc tggtagcgta tccgagaaag aacgactgct actcaagctg ctgatgcagg
      361 gaatgtctgt aacagaaata tcacagtaca gaaatcgcag tgcaaagaca atttcacatc
      421 aaaagaaaca gctctttgag aaactgggga ttcagagcga tattactttc tggcgcgata
      481 ttttctttca gtacaatccg gagatcatat ccgccacggg tagtaatagt catagatata
      541 ttaatgataa tcactatcac catatcgtca cgcctgaagc catcagtctg gcgttggaaa
      601 accacgaatt caaaccgtgg atccaaccgg ttttctgcgc gcagactggg gtactgacgg
      661 gctgtgaggt gcttgtccgc tgggaacatc cacaaacggg aattatccca ccggatcagt
      721 ttattcctct ggcggagtca tccggtctta ttgtcataat gacccgccaa ctgatgaaac
      781 agactgcgga tattctgatg ccggtaaaac atttgctgcc ggataatttc catattggta
      841 tcaacgtctc ggcgggttgt tttttggccg cgggatttga aaaagaatgc ctgaacctgg
      901 tcaagaaatt aggaaacgat aaaatcaaac tggttctcga gctaacggaa cgtaacccta
      961 ttccggtaac gccagaagcc agagcgatat ttgacagcct tcatcagcac aacattacct
     1021 ttgcgctgga tgactttggt acgggttatg cgacctatcg ttatctgcag gcgtttccgg
     1081 tcgattttat taaaattgac aagtcgtttg tgcaaatggc gagtgtcgac gaaatatccg
     1141 gtcatattgt ggacaatatt gtcgaactgg cgcgtaagcc tggtctgagt atcgtggcgg
     1201 aaggggtaga aacccaggag caggcggatt taatgatcgg taaaggcgtt cactttttgc
     1261 agggctattt gtactctccg ccagtaccgg gtaataaatt tatctctgaa tgggtaatga
     1321 aagcaggtgg ttaatgtaaa ccgctattca cagcgcatcg ggaggttggc agcgattaat
     1381 tctcccgatg cagtaaatcc tgatagatcc ccgttaatat gccatttggc gcaaattgtt
     1441 ttttaatcca ctgcgtcacc tgacccgtag cagaatgctg agtcgccagc aacatgcgcg
     1501 aatcctggcg cggattatgg atttgtcggg taaccagcag ggattgcgcc atcgcctcac
     1561 ggaccatgta atccggcaaa aaaccaattc cttcacctaa aatctggcac tgacatttgg
     1621 tgttgaaatc tggcaccaga atcgactcct gcccatgcag caaccagccc acttttttat
     1681 taatcgtatg cgcggtgtcc tccaccatga tattagggta gagacgcagc tggctttcgg
     1741 cgatgggttc cggcacaaag gctaacgggt gatccggggc gatagcaaaa gcccagcgaa
     1801 tcgcgccaat ttcagtgtaa tcaataccgc cgccgtccag tagtgtatcg ggtgcgccga
     1861 tggcgatatt ggcctgatta ttaataatcg catcccagac gccgttatac acttcggtcg
     1921 tcacggtaat ctggcaggta ggaaactgct ttttcagcac ctgtaataag cgcgcggtgt
     1981 gtttgggcgt ataaagcagc tgattaatac aaatgcgcac acgcgcttct atgccctgcg
     2041 aaatagtatc tatgctacgt ttgatggcat aaaaatcatt cagcaggtcg gtagctttgc
     2101 ggaaaaaata acgcccggac tccgttaact caatgctgcg ggtactgcga gtgaacagca
     2161 ccacatccag ccccgtctcc atacgcttaa tggtgtagct aatggcggaa gtggttaaac
     2221 ccaactcttc tgcggcttta ctgaagctgc caaaacgcgc agcggtagta aatgccaaca
     2281 gattctcttc ggtaaaaatt gagttcatta aaccattccc gccattatct actatgaatt
     2341 tctccagcta tgactgtaga ggtatcggtt aaagatagat aatcattttt gaataacttt
     2401 taatacccgt cgcgtttagt cgtcgcttcg cttaattttt gtccaccata ttctgaaatt
     2461 atccccacgc agttacccgc cagaaaaccc agtaatgtca ccgtccagtt cataccgctg
     2521 gcaaaaaaca gcgtcatgcc gagaaagcca ccgggaataa atgacagtaa ccagaaacgc
     2581 ccttgccaga ccaccaccgc cgagaagggt acggtagtaa ttacgctcgc ccagaacgcg
     2641 gacagtccac ttacagaggc cagccaacca gcagccagcg cggcgaaaaa cgcccacacc
     2701 atcccggaat aattaaccgc cagacttctg ataaaccccg atttaccgcc gcctgcggca
     2761 taaaaactgc aaaaggcgac gaagccaatg gttcctaata attcccatcc ctgagagagc
     2821 ccgacatgtg aagacactaa ctgccaaagc cctgcacaaa taccgacggt gacacccgtt
     2881 gccgtgagtc cgttcatctt gttcccctct ggttagacat ggacgaaagc tactcttaca
     2941 tgtgcagact atagagaaat aaaacctgat tgagtgaaag ggtatcgggt caaagaaaca
     3001 aatattgcac taccgcacac tgcgaaaaga ttgttgaaca tcattcaaca aaaaggcgaa
     3061 atacggcgaa ttaaatttaa gcgggttctt ttatcagatt atcgttgcta ttctcaattc
     3121 atcgaaaaca gccacaaaaa ccaggggaat gtgaatgagt ataaaaaatc tacctgccga
     3181 ctatttattg gctgcacaac agggtgatat cgataaagta aaaacctgcc ttgcgctggg
     3241 tgtcgatatt aatacctgcg atcgtcaggg gaaaacggca attacgctgg caagtttata
     3301 tcagcaatat gcttgcgttc aggcattaat tgatgccgga gcggatatta ataagcaaga
     3361 tcatacctgt ttaaatcctt ttttaattag ctgtctgaac gatgatttaa cgctactacg
     3421 aattatttta ccggctaaac ctgatcttaa ttgcgtaacc cgttttggcg gtgtcggcct
     3481 gacgcctgcc tgtgaaaaag gccatttaag tattgtaaaa gagcttttgg cgcatacgga
     3541 aattaacgtt aaccagacca accatgtagg ctggacgccg ctactggaag cgattgtgct
     3601 taatgatggc ggtattaaac agcaggcgat tgtccagcta ttgctggaac acggtgccag
     3661 tccgcacatg acggataaat atggcaaaac gccgctggaa ctggcgcggg aacggggctt
     3721 tgaagagatt gctcagttgc tgattgccgc aggtgcataa accgggaggc ttgctatcaa
     3781 cacaccagaa aaacggtgtg tgtgggcgct aactgcggat gcggattttc tggcgcagcg
     3841 ggggcaagga caggttgaac aggtctttgc cagagcggta aatatcgcac tcccggctcg
     3901 ccagcagttg ctgacgctgc tttgtgaaga gtacgacaat gcgccaaaca gttgtcggtt
     3961 ggcactcact cactttgatg gtctgtttcg gcatggtgat aaggttcagt ttgacgatca
     4021 aggcattacg gttggtcaac atcttcatat agagatgagt cgttgtcggc gttggctgtc
     4081 cccaactttg caaatgaccg ctgaaaattt tcaccttatc gcctggcaac agtggtacga
     4141 cattattcat cagcacctgg gggaaaatga aaccctgttt aattatcgcg gcgataatcc
     4201 gttttatcag gcattaaata aagaattaca tattaaacga caggcagtta ttcaggccgt
     4261 aaacgataaa caaaatatcg cctcagcggt cgctaatatg atggggttag ggattggcct
     4321 tacgccatca gccgacgatt atctgaccgg gctggtactt attttattta tttgtgggca
     4381 tccggcagaa aaatacaaag aggaatttta tctcggtttg caacgcggca gaaaaaatac
     4441 cacgttatta agtgctatta cgctggaggc cgcattacaa caacgctgcc gggaaaatat
     4501 tcatcgtttt attcacaata ttatttatga catccctggg aacgcaactc aggcaataga
     4561 aaaaattaaa catattggct ccagttccgg ctgcgacatg ctgtatggca tggccgatgg
     4621 ttgtgcgctg agccaaacct acggagggaa ttatgtcagt taaaatagtc attaaaccga
     4681 atacctattt tgattctgtc tcgctgatgt ctatctccac gcgtgcaaat aaactcgacg
     4741 gcgtcgagca ggcatttgtg gcgatggcga ccgaaatgaa caaaggtgtg ctgaaaaatt
     4801 taggactgct gacgccggag ctggaacagg cgaaaaacgg cgacctgatg attgtcatca
     4861 atggtaaatc gggtgcggac aatgagcagt tgctggcgga gattgaagaa ctgttcaaca
     4921 ccaaagcgca aagcggctcg cacgaggcgc gttacgccac tattgccagc gccaaaaagc
     4981 atatcccgga aagtaacctg gcggtgattt cggtcaacgg tctgtttgcc gctcgcgaag
     5041 cgcgtcaggc gctgcaaaac gatctcaacg tgatgctgtt ttccgataac gtctcagttg
     5101 aagatgaact ggcgctcaag caactggccc acgaaaaagg gctgctgatg atggggccag
     5161 actgcggcac ggcgattatc aacggtgcgg cgctctgctt tggtaacgcc gtgcgtcgcg
     5221 gcaacatcgg tattgttggc gcatccggca ccggcagcca ggagctgagc gtccgcattc
     5281 atgaatttgg tggcggcgtt tcacaactca tcggcaccgg cgggcgcgac ctgagcgaga
     5341 aaatcggcgg cctgatgatg ctcgacgcca tcgggatgct ggaaaacgat ccgcaaactg
     5401 aaattatcgc gctgatttca aaacctcccg cgcctgcggt ggcccgtaaa gtgctggaac
     5461 gcgcccgcgc ctgccgcaag ccggtggtgg tctgcttcct cggtcgtggc gaaacgccag
     5521 tggatgagca ggggctacag tttgcccgcg gcagcaaaga ggcggcgcta aaagcggtga
     5581 tgctctccgg cgtgaaacaa gaacatctcg acctgcatac cctgaaccag ccgttgattg
     5641 cggatgtgcg tgcgcgtctg caaccgcagc agaaatacat tcgtggcctg ttctgcggcg
     5701 gcacgttgtg cgacgaaacc atgttcgcgg tgatggaaaa acatggcgat gtctacagca
     5761 acattcagcc cgatccggaa ttccgcctga aagatatcaa ccgcagcatc aaacacacct
     5821 tcctcgactt tggcgatgac gacttcacca acggcaagcc gcatccaatg attgacccca
     5881 ccaaccgcat cagtcgcttg atcgaagagg cgcgcgatcc agaagtggcg gtgatcgtga
     5941 tggattttgt gctcggattt ggatcgcatg aagatccggt cggctccacc atcgaggcga
     6001 tcaaagaagc gaaagcgatc gccgctgccg aaggacgcga gttgatcatt ctcgcctatg
     6061 tgctgggtac cgatcttgat acgccatcgt tagaacaaca aagccagatg ctgcttgatg
     6121 ccggagtgat tctggcgagc agcagcacca ataccggatt gctggcgcgt gaatttatct
     6181 gcaaagggga ggaagcctga tgagccagtc actgtttagc caaccattga acgttattaa
     6241 cgtcggtatc gccatgttta gcgatgacct gaaaaagcag catgtagaag tgactcaact
     6301 cgactggacg ccgccggggc agggcaatat gcaggtggtg caggcgctgg ataacattgc
     6361 cgattcgccg ctggcagaca aaatcgccgc cgctaaccag caggcgctgg agcgcattat
     6421 ccagtcgcat ccggtgctga ttggttttga tcaggcgatt aacgtggtgc cgggcatgac
     6481 gccgaaaacc attcttcacg ccgggccgcc gatcacctgg gaaaaaatgt gcggcgcgat
     6541 gaaaggcgcg gtcaccggag cgctggtgtt cgaaggactg gcgaaagatc tcgacgaggc
     6601 ggctgacctg gcggcttccg gggagatcac cttctcgccg tgtcacgagc acgactgcgt
     6661 gggatcgatg gcgggtgtta cctcggcctc gatgtttatg cacatcgtaa aaaacaaaac
     6721 ctacggcaac atcgcttata ccaacatgag cgagcagatg gcgaagattt tgcgtatggg
     6781 cgctaacgac cagagcgtga ttgaccgcct gaactggatg cgtgatgtgc agggaccaat
     6841 actgcgcgac gcgatgaaaa ttatcggcga aatcgatctg cgcttaatgc tggcgcaggc
     6901 gctgcatatg ggcgatgagt gccataaccg caataacgcc gggacgacgc tgctgattca
     6961 ggcgctgacg ccggggatca ttcaggcagg ttattccgtc gagcaacagc gcgaagtgtt
     7021 tgagtttgtt gccagtagcg attacttctc cggcccgacg tggatggcga tgtgtaaagc
     7081 ggcgatggat gcggcgcacg gcattgaata cagcaccgtg gtcaccacca tggcgcgtaa
     7141 tggcgtcgag ttcggcctgc gggtcagcgg cctgccgggg cagtggttta ccggcccggc
     7201 gcagcaggtc atcggcccga tgtttgccgg ttataagccg gaagattcag ggctggatat
     7261 cggcgacagc gccatcactg aaacctacgg cattggcgga tttgccatgg cgaccgcgcc
     7321 tgcaatcgtt gcgctggtgg gtggcacggt ggaagaggct atcgatttct cccgtcagat
     7381 gcgcgaaatc acccttggcg aaaaccccaa cgtcaccatt ccgctgctcg gttttatggg
     7441 cgtgccgtcg gcaatcgaca tcacccgcgt gggtagcagc ggcattctgc cagtgatcaa
     7501 caccgccatc gcccataaag atgcgggcgt cggcatgatt ggtgcgggca ttgtgcatcc
     7561 accttttgcc tgcttcgaga aagccattct tggctggtgc gaacgttacg gcgtctgacc
     7621 tacatgttca tgccggatgc ggcgtgaacg ccttatccgg cccacgaatg gaacgagaat
     7681 ctgtaggcct gataagcgta gcgcatcagg catttgtcac cattgccgga tgcggcgtga
     7741 acgccttatc cggcctacaa gtggcgcaag aatttgtagg cctgataagc gtagcgcatc
     7801 aggcatttgt caccattgcc ggatgcggcg tgaacgcctt atccggccta cgaatggcgc
     7861 aagaatctgt aggcctgata agcgtagcgc atcaggcatt tgtcaccatt gccggatgcg
     7921 gcgtgaacgc cttatccggc ctacgagtgg cgcgagaatc tgtaggcctg ataagcgtag
     7981 cgcatcaggc atttgtcacc attgccggat gcggcgtgaa cgccttatcc ggcccacgag
     8041 tggcacgaga atttgtaggc ctgataagcg tagcgtatca ggcaatttgg cgttggtcat
     8101 aaccccatca ccctctgtag cggacataac aacatgaaag aacttgtggt cgttgccatt
     8161 ggtggcaaca gcattatcaa agataacgcc agccagtcga ttgagcatca ggcggaggcg
     8221 gtgaaagccg tcgccgatac ggtgctggaa atgctggctt ccgattacga cattgtgctg
     8281 acccacggca acgggccgca ggtcgggctg gatttacgcc gtgcggagat tgcccacgag
     8341 cgcgaagggc tccccttaac gccgctggcg aactgtgtgg cagatacgca gggcggcatc
     8401 ggctatctga tccaacaggc gctgaataac cggctggcgc gtcacggcga gaaaaaagcc
     8461 gtcaccgtgg tgactcaggt ggaagtggat aaaaacgatc cgggatttgc ccatcccacc
     8521 aagcccatcg gcgcattctt tagtgacagc cagcgtgacg aattacaaaa ggcaaaccct
     8581 gactggcgtt ttgttgaaga tgccggacgg ggctatcgcc gcgtggtcgc ctcgccggaa
     8641 ccgaaacgta ttgtcgaagc acccgccatt aaggcgctga tccaacaagg ctttgtggtg
     8701 attggcgcgg gcggcggtgg aattccggta gtgcgtactg aagcggggga ttaccaaagc
     8761 gtggacgcgg ttatcgacaa agatctctct accgcgctgc tggcccgtga aattcacgcc
     8821 gacattctgg tgatcaccac cggcgtggaa aaagtgtgta ttcattttgg caaacccgag
     8881 cagcaggcgc tcgatcgggt ggatattgcc accatgactc gctatatgca ggaaggacat
     8941 ttcccgcccg gcagcatgtt gccaaaaatc atcgccagcc tgacgttcct ggaacagggc
     9001 ggtaaagaag tgattatcac cacgccggaa tgcctgcccg cggcgctgcg tggcgaaacg
     9061 ggcacccata ttattaaaac gtaaggacgt aagatgaaag aaagcaatag ccgccgtgaa
     9121 tttctgagcc agagcggtaa gatggtcacc gccgccgcgc tgtttggtac gtctgtgccg
     9181 ctcgcccatg cggcagtctc tggcacaacc cactgcgacg cgaataacac catgaaaatc
     9241 actgacccgc attactatct cgacaacgtg ctgctggaaa ctggatttga ctacgaaaat
     9301 ggcgtggcgg tgcagacccg cacggcgcgc cagaccgtgg agattcagaa cggcaaaatt
     9361 gtcgcgctgc gcgagaataa gcagcacccg gatgccacgc tgccgcacta tgatgctggc
     9421 ggtaagctga tgctgcccac cacccgcgac atgcatattc atctcgacaa aacgttttac
     9481 ggcgggccgt ggcgctcgct caatcgcccg gcaggcacca ccatccagga catgatcaaa
     9541 ctcgagcaga aaatgctgcc ggagctgcaa ccgtacactc aggagcgggc agaaaaactg
     9601 attgatttat tgcagtcgaa aggcaccacc attgcccgaa gccactgcaa tatcgaaccg
     9661 gtttccggcc tgaaaaatct gcaaaatttg caggcggtgc tggcgcgacg tcaggcgggc
     9721 tttgagtgtg aaatcgtcgc cttcccgcag cacggtttgc tgctgtcgaa atcggaaccc
     9781 ttaatgcgtg aagcaatgca ggcgggggcg cattacgtcg gcggcttgga cccgaccagt
     9841 gttgatggcg cgatggaaaa atccctcgac accatgttcc agattgcgct ggactacgac
     9901 aaaggcgtcg atattcacct gcacgaaacc actccggcag gcgtggcagc catcaattat
     9961 atggttgaaa cggtagagaa aacgccgcaa ctgaagggca agctgaccat cagtcacgcc
    10021 ttcgcgctgg ctacgctcaa cgaacaacag gtagatgaac tggcgcaccg gatggcggcg
    10081 cagcaaattt ctatcgcctc gacggtgccg attggcacgc tgcatatgcc gctcaaacag
    10141 ttgcacgaca aaggcgtaaa agtgatgact ggcactgaca gcgttatcga ccactggtcg
    10201 ccttatggtc tgggcgacat gctggaaaaa gccaatctgt acgcgcagct ctatattcgt
    10261 cctaacgaac agaacctctc ccgttcgctg tttttagcca ctggagatgt attgccgctc
    10321 aacggaaaag gcgagcgtgt gtggccaaaa gcgcaggatg acgccagctt tgtgctggtg
    10381 gacgcctcct gttctgccga ggcggtggcg cgtatctcgc cgagaaccgc aacgttccat
    10441 aaagggcaac tggtgtgggg gagtgtggca ggttgatgcg catttgaaac gcctgcaacg
    10501 gtgaatagta agagatttaa gccccaggga gtggggcggg aatggtttgc taatgaatga
    10561 tcctgaactt tataatctcc cggcataatt tccaggggat tatttgagag aaatacacaa
    10621 aagtgagttt taacaaaggt atttatcagg ccataaatgt gacctgcttc ggataaaaaa
    10681 gattcagtat cgaaaaacca atgatactct tgctgatggt taccaacagt aattaagatg
    10741 tgatttgttt ttccgtaatc gcaaaaatat tgccatgacc ataaaagaaa aattctcaca
    10801 aaaatatccc catgcttcct tttgtacttt tggcgattca gccgcgttgg ccgatcacct
    10861 cgcaacgttg attgcgactg gtattaaaac agcttcctgt ggttcgctgg cgggatgtat
    10921 tgaagacaac gcatttccga tgattggtga gtataagatt gtagaaaata gccgtggtga
    10981 gcctgtatgt gttatcagag tgattggcct gcatttactg cgcttttctg atgtcaccgc
    11041 ggagcttgcc cgtaaggaag gtgaaggtga cctcagtctt gaatattggc gtaatgaaca
    11101 ccgtcgtttt ttccaggcag aaggaagcta ttcaccagaa atggacgtta tctttgaaga
    11161 atatgccctt attgacgttg tgtagataac tatttatcta cacgaaataa gctcacaaac
    11221 ataaaatatc gctaattttg tttattttgc tgttttttac gtgatgaatg tcacacttat
    11281 tattgctgcc cgttttgtcc atatttataa ccatattttt aatagctgcc tgaaatagag
    11341 ctgtttattc tgcataaaaa tattcgaaaa ttcttattct ctccagcgcg ctaatttggg
    11401 cgaacacttc ctgactaccc tgcaatgagg ctgaaatgat gaataaacgt tttgttatca
    11461 atatggtgtc ttcgctgtta ttaggcgctg cgctaatttc tgcgccgctc caggcagcgg
    11521 aaaaagtcgt ggtgaatatt tctaaagtcg atggtatgcc gtggtttaat cgcatgggcg
    11581 aaggtgtggt tgaggcgggt aaagcctttg gtgttaatgc ctcacaggtg gggccttcaa
    11641 gtactgatgc tccgcaacaa gttaaaatta ttgaggattt aatcgcccgt aaggttaatg
    11701 caattactat cgtgccaaac gatgctaacg tgctggaacc ggtattcaaa aaagcgcgag
    11761 atgccggaat tgtggttctg accaacgaat cccccggtca gccaagtgct aactgggata
    11821 tcgaaatcat tgataacgaa aaatttgccg ctgaatatgt tgagcatatg gcgaaacgca
    11881 tgggtggcaa gggcggctat gtgatttacg tgggtagttt gaccgtgccg cagcataact
    11941 tatgggctga cttgctggtg aaataccaga aagagcatta cccggatatg catgaagtta
    12001 cccgccgcat gccggttgcc gagagtgtgg atgactcacg tcgtaccaca ctggacctga
    12061 tgaaaaccta tcctgacttg aaagcggttg tctcctttgg ttcaaacggt ccgattggcg
    12121 cgggacgtgc ggtgaaagag aaacgggcga aaaataaagt cgcagtctat ggcatgatga
    12181 tcccttcaca ggcggcatca ctgattaaaa gtggtgatat caccgaaggg attacttacg
    12241 atccggcctc tgcaggttac gcactcgcag ccgtcgccag cacattgctg aagggtgaag
    12301 agatcaagcc tggattagaa atgcagaatc tgggtaaggc cgacgtagat atggataagc
    12361 gaattatccg tttccataag gtattactgg tcaacaaaga caatatcgac tcactttatt
    12421 aatccatttc ttcggggcga aagccccgat tcttattgcc aggggatgtt atggaaacct
    12481 tcctttccct tcgtcatatc aacaaaacgt ttcatgccac gcgagcgctg cgcgatgttt
    12541 ctctcgactt tatgtcgggt gaagtgcact gccttgccgg gcaaaacggc tgtggtaaat
    12601 cgacgttgat taaaattatg tccggtgttt atcgcccgga tgagggagcg gaaattacgc
    12661 ttggtgggaa aaactggtca aagctgacac cagccgcttc ggtggcgcag gggattcagg
    12721 tgatttatca ggacctctct ttatttccta acctgagtgt ctgggagaat atcgccgtga
    12781 atcactatca ccacggtctg tttgttaacc gccgtcgtct gcgtgaggtg gcgcaggcgg
    12841 caatgaccag tattaacgtc acattgccac tggatacact cgtttctgag ctttcaattg
    12901 cccgctgtca gctggtggct atttgccgtg cactggcgca ggacgcacgg ttgatcgtga
    12961 tggatgaacc aaccgcttcg ctgacccatc aggaagtgca agggttatta caggtggtgc
    13021 atcaattgcg tgaacgcggg atctgcgtgg tctttgtcag tcatcgtctg gaagaagtga
    13081 tggaagtttc cgaccgtatt tcagtgctga aagatggtga gctggtcggg acttttccgg
    13141 ctgcagagat gaccacaaaa caactcggtt tcctgatgac cggccaggag tttgaatatc
    13201 aggtgcgaga gttgtggcag ggaaaatcca gcaccccggt gctggaggtg cgtaatttaa
    13261 gtcgtcatgg ggaatatcta aatattaact tgcgggtgga agcgggcgaa gtggtatcga
    13321 ttgttggcct gcttggtgcg gggcgcacgg aattatgtct gagtctgttt ggtatgaccc
    13381 gaccggatgc tggcgagatc ctcatcaacg gccagccggt tacactgcat agtaaccagg
    13441 acgccatccg ccacggtatt ggttatgttt cggaagatcg catgtcgcgc ggtttggtga
    13501 tggcgcaatc tattgaagac aacatcatta gcactgtttt tcacaaggta aaagatcgct
    13561 tcggctttct gagtgaaact aaagtgcgtg acctggtgga caggctaatc aaggcattga
    13621 ccatcaaagt ctctgatcct catctcccgg tgaatacgct ttctggtggt aacgcgcagc
    13681 gagtatcgat cgccaaatgg ctggcgattg ggccacgact gttgattctc gatagcccga
    13741 cagtcggtgt tgatatcgcc aacaaagcag gaatatacgg cattatcagc gatctggcgg
    13801 ctcatggcat tgcggtattg atgatctgcg atgaaattga agaagcgtgg tatcaaagcc
    13861 accggatttt ggtaatgcaa aaaggtcaga tcacccatag cttcctgcct gacagcagtt
    13921 ctcaggccag gattgcggag gtggtaaatg gctgaactga aaaaacgcca tgaattctgg
    13981 ctggcattac tgattgtggt gatgtttgtt ggccttgcct ggcgcagcga cgagtttctg
    14041 acattcggta atttgtacga tctcgccaat aactatgcca tgttgactat tctcgcctgt
    14101 ggtttgtttg tggtgttgat ttccggtgga attgatattt cgtttccagc aatgaccatc
    14161 attgcgcaat acggcatggt gctgttgctg caaaaaattg gtggcaactt cgctgtcgcg
    14221 tttgcactgg cgggctgcat cggcatttta cttggcttaa ttaacgcctt actggttaat
    14281 cgcctacggg tgccttctat catcatcact atctcgacgc tgaatatttt ctatggcctg
    14341 ctgttatggt tgagtaaagg tgtgtggctg tacgactttc cgccgtggtt tgagcagggg
    14401 gttatgttgt tcaagtatac cgatgctgat ggctatgact atggccttgg tctgccgctg
    14461 atcgccatga ttacggtggt gctgctaaca gcgtttatca tgaatttcac cagtgtaggg
    14521 cgcaaaattt atgcccttgg cgggaaccgc gaatcagcca gtcgcatcgg ctttagcgtg
    14581 ctgaaactgc aacttttcgt ctatggctat atgggattga tgtctggcgc tgcgggtgta
    14641 gtgcagtcgt ggacggtgat gactgtcgcc cccgactctc ttctgggtta tgagctgaca
    14701 gtactggctg cggtggtgct tggcggcact agtttgctcg gcgggcgcgg cacgttaacc
    14761 ggtactttgc tcggcgtggt gttgttggca gtgatgcaaa acgggctaaa tttattggga
    14821 gtctcgtctt actggcaaac attgatcacc ggcatcatca tcgttgccag cattagtgcc
    14881 acggcgtgga gtcagcatca gaaccggagt ctgctatgaa aaaatcgtgg cgtaataacg
    14941 ttgagtttta cttgatcggc ctgctggtgc taactgttgc cgcgttcagc attactatgc
    15001 cggagatttt ctggtcgata agtaatttcc agtccgtcgc ttcgcaaatg cccgtgctgg
    15061 gcattctggc gttggctatg gcggtgacca tgctttgcgg tggcattaac ttgtcaatta
    15121 tagccactgc taacgcctgt tcactggtga tggcgtgggt ggcgactcag tatccaccgg
    15181 gtattgctac ggttgtcgct acgttactgg cgggcgctgg cgcggcggtg ataatcggtt
    15241 tatgtaacgg cgtattgatt gctggtattc gagtgtcgcc aattcttgca acgcttggga
    15301 tgatgacgtt gctcaaaggg ataaatattc tggtgacagg tggtagcgcc attgccaatt
    15361 atccctcatg ggtgctgtgg ttgaatcatg cgcaatggtt cggtattccg ctaccgatgt
    15421 ggttgtttgc cgcagtggca ctggggctgt ggatattgct ggagaaaacg ccgctcggta
    15481 aagccattta cctgattggg tctaacgaac gagcaacact ttactccggc attaacactc
    15541 gtcgggtgct gatttgggtt tatgtcattt ccgccttgct ttgtgcggta gcggcatttt
    15601 tgatgatgtc gaaactcaac tcggctaagg cctcttatgg cgaatcgtac ttgctggtgt
    15661 caatcctcgc ggcagtactc ggcggggtta atccggacgg cggcagcggg cggataattg
    15721 gtatggtgct ggcgctgttt cttctgcaaa ttattgaaag cggcttcaat attctcggta
    15781 taagccccta cctgacgatg gccttatggg gaacgttgct gctctgtttc attcaggctc
    15841 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
    15901 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
    15961 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
    16021 ccggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
    16081 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttatccct gcgtgccggg
    16141 tcatgaaatt gtggggtgtg tggtagccgt tggtgatcag gtagaaaaac atgcgctggg
    16201 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgaaga
    16261 cgggctggaa aactactgtg atcacatgac cggcacctat aactcgccaa cgccggatga
    16321 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
    16381 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
    16441 caccacgtat tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
    16501 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
    16561 ggtggcattt accacttctg aggcaaaacg cgaagcggca aaagccctgg gggccgatga
    16621 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
    16681 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
    16741 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
    16801 cctgatcatg aaacgccgtg cgatagccgg ttctatgatt ggcggcattc cggaaactca
    16861 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
    16921 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
    16981 tatcgataat cgcacactaa cagactgaaa aaattaataa ataccctgtg gtttaacata
    17041 ttaacttcgc tctccactta actttttagt taaggagagc gaaataatat caaagtagca
    17101 gtaaaaccta taatgtaaat tcaaattgtt aaattaactc cctccagtac acaatacttc
    17161 acacgttagt tatgagcgat ttctgatagt gcctggttta atcagagctt tattatctgc
    17221 gacgtttatt tttatttaag agagatcgcg atgatatcat taaaagctcc gcacaataat
    17281 ttgatgccat atacgcaaca aagcatactt aatacggtta aaaacaatca gttaccagag
    17341 gatatcaaaa gctccctggt ttcctgtgtc gatatattca aggttttgat taaacaatat
    17401 tatgattatc cttatgattg tcgtgatgat ttagtcgatg acgacaaact cattcatctc
    17461 atggctgccg tacgagactg tgagtggtca gacgataacg cactcaccat aaatgtgcag
    17521 tttaatgatt tccctggatt ttatgactgg atggattacc ctgatcaccc ggttaagttt
    17581 gtttttcgca tacttgaaaa tcaaaaaggg acagtttggg tatatgacca ggatgatgcg
    17641 tttcttgata ttaaagcaaa cgtgcaggct ggacgcttta ccgggcttaa gaaattagtg
    17701 cagtttatcg attcagtgcg aacagattgc aaatgtattc ttcttgaaca ccatatgcct
    17761 ttgctgagac ttttccctaa agggaaagag tgtatgcatg tggaaaaatg gcttcgcgaa
    17821 atgtcgtcta ttccggagac agacgcgccc attaagcagg cgcttgcgca tggattatta
    17881 ttacacttaa aaaatatcta ccctgttttt cctgaatcac tggtgatgtt attgctctct
    17941 gttctggacg taaaaacata cagagatgat gcgcgattaa atcaatggat atctaataga
    18001 gtccaggaat taggtgacag atattatcct gtaaataagc acgttaaaat ccgctacact
    18061 ctttaggtca gaaagggttc cacctgcaag ttgttattcc accagagagt gaatcgcaat
    18121 gcaggtaatc atttcatctg tatatctgta tgccaactgg cattccatct cttttttcgc
    18181 cgatattttg ttgtgatcgg cgacacttcg cttaaaaaag caccagtagt ggtttcgcag
    18241 ccatgcggtg tataaaaaat gaactcatgc agatgttttg tgaatgtgtg ggttgacatt
    18301 catatgaaaa aaatcataat tccatcatgt ttgtgtgggg tcttttctgt accttacgca
    18361 tcgcactcaa gcctgacaga aaatatgctg taaggctcat atcaaaaacc gccattagct
    18421 catcaggaag agcagacgac aaccaggatt gttgtatggt acggggttcg aggcctcgat
    18481 ggcggtccaa cttttcaaaa ctcttctgaa tcagatcccc ttattatcat ccctgcaaag
    18541 tggaacattg ccattgtttg gctattcagg acgggtcata ccaatgaaaa aagcccacac
    18601 aggggagagt gggctgaaat gggaagctaa agactcaagt aaacttatcg gaaataagga
    18661 ccacgcatta cgggggctat catcgccgat ggtcgggatt cggcaaatca ggttgattac
    18721 gtagagagta aattatctgc tcaccgctgc gtcacacctt cgtaaatcag gcgtagcgcg
    18781 aatacaccaa taattgcacc aataacccga ctggcaacgc gttgcatacg cccataagca
    18841 cgacgcacag cgggcaaaga aaacgcctga ctaagaaaga ctcgccagat aactgatgcg
    18901 agcacaatcc ccgcccaggc cattaaacgt gcccatgttg gtgtttcggc atttaatgtt
    18961 actgagaaaa tactaataaa aaataaaacg gtttgcgggt tagagagatc ggtaattaat
    19021 ccgcggcgaa aaaagacata ccagggggcg ctaatcggtt gttgtagtgt gctcatttgc
    19081 ggtgttgact ggcggcgcat gctgcaccac gcaaaccata agagataagc gccgccgacg
    19141 attctgataa gcgaaaaaat ctcctcacac tgcgtaatta gcgttgcaag accaaacaaa
    19201 cccaacccgg aataaaatgc atcgcccagc gccacgccca gcccggtcag cacccctgcg
    19261 cgtcgaccgg aagccaggct ggtttgtact accacaaaga gattggctcc cggattaaaa
    19321 aaagtaatca cgaacagtcc tacggtcagg taaacggcgt gcaaaggatc catagtgatt
    19381 tcatccataa ataagtgaag taacttcatc atatttctac caaaaataat cgggtgcgag
    19441 agagatcaca aagtgtctta tttccggtta ctggcgttta tgccctgact gaactaatta
    19501 ttaatcaacc caataatgtg ggtgggtgat agtgtgataa caactctgga gccgtaatat
    19561 gaaaataatc tctaaaatgt tagtcggtgc gttagcgttt gccgttacca atgtctatgc
    19621 cgctgaattg atgactaaag cggaatttga aaaagtcgaa tcgcaatatg aaaaaatagg
    19681 tgatatttca accagtaatg aaatgtcgac cgcagatgcg aaagaggatt tgatcaaaaa
    19741 agcggatgaa aaaggggctg atgtgttggt actgacctct ggtcaaactg acaataagat
    19801 ccacggcacg gcaaatattt ataagaagaa gtaattctga accctatgta aacatcccga
    19861 tgcgtaagtt tatcggtgat catcatctat tgaaatttat gccggataaa gcgttcgcgc
    19921 tgcattcggc agttcagttt ttcagccgcc gccagaacgt cgtccggctg atgcctaaat
    19981 aattcgccgc tgctgtttta tcgcctttaa atttctccag tgcctgttgt ggtgtcagta
    20041 agcgtggagc gggagttttc gccgactcgc gcgccagttc cggcagtagc agctgcaaaa
    20101 attgcggcgt taaatccggc gtcggttcca cacttaaaaa tagcgccagt cgctccatca
    20161 tattgcgcag ttctcgaata ttgcccggcc agtcgtagcg cagcagcaca gtttcacttg
    20221 cctgtaaccc ctggcgtaat gcagcagaaa atggggcgga gagcgccgcc agagacactt
    20281 tcaaaaaact ttccgccagt ggcagaatat ctgccacccg ctcgcgcagt ggtggcaatt
    20341 gcagacgcaa aatactcagc cgataaaaca gatcacggcg aaactgccct tgccgcatat
    20401 cttcttccag attgcagtga gtggcgctaa tgacccgtac atctaccgga acaggctgat
    20461 gcccgccgac gcgggtgacc tctttttctt ccagcacccg cagcagccgg gtctgcaaag
    20521 gtagcggcat ttcgccaatc tcatccagaa acagcgtgcc accgtgggcg atttcaaaca
    20581 gcccggcacg acctccgcgt cgcgagccgg taaacgcccc ttcctcatag ccaaacagct
    20641 ccgcttccag cagcgattcg gcaatcgccc cgcagttgac ggcaacaaac ggatgcgact
    20701 ttttgccctg tcgcgcatcg tggcgggcaa aatattcccg atgaattgcc tgggccgcca
    20761 gctctttgcc cgtccccgtt tccccctcaa tcaacaccgc tgcactggag cgggcataca
    20821 gcaaaatagt ctgccgtact tgttccatct gtggtgattg accgagcata tcgcccagca
    20881 cgtaacgagt tctcagggcg ttgcgggtgg catcgtgagt gttatggcgt aacgacatgc
    20941 gcgtcatatc cagcgcatcg ctgaacgcct ggcgcacggt ggcggcggaa tagataaaaa
    21001 ttccggtcat tccggcttct tctgccagat cggtaatcag ccccgcaccg accaccgctt
    21061 cggtgccgtt agctttgagc tcgttaatct gcccgcgggc gtcttcttcg gtaatgtagc
    21121 tacgttgatc gaggcgcaaa ttaaaggttt tttgaaacgc caccagtgcc ggaatagttt
    21181 cctgataagt gacaacgccg atagaagagg tgagttttcc ggcttttgac agtgcctgta
    21241 acacatcgta gccgcttggt ttaatcaaaa taactggcac tgacaggcgg cttttcaggt
    21301 acgcgccgtt agagccagca gcgatgatgg cgtcacagcg ttcgtttgcc agtttcttgc
    21361 ggatgtaggt cactgctttt tcaaagccaa gctgaatagg ggtaatgttc gccaggtgat
    21421 caaactcgag gctgatatcg cgaaacagct cgaacaggcg cgttacagat accgtccaga
    21481 taaccggttt gtcgtcattc agccttggtg gatgtgccat agcgcaccgc aaagttaaga
    21541 aaccgaatat tgggtttagc cttgtttcat aattgttgca atgaaacgcg gtgaaacatt
    21601 gcctgaaacg ttaactgaaa cgcatatttg cggattagtt catgacttta tctctaacaa
    21661 attgaaatta aagatttaat tttattaagg taattgtggc acaccccttg ctttgtgttt
    21721 atcaacgcaa ataacaagtt gataacaaag gatgggctat gtctctacac tctccaggta
    21781 aagcgtttcg cgctgcactg tctaaagaaa atccattgca gattgttggc accattaacg
    21841 ccaaccatgc gctgttggcg cagcgtgccg gatatcaggc aatttatctc tccggcggtg
    21901 gcgtggcggc agggtcgctg gggctgcccg atctcggtat ttctaccctt gatgatgtgc
    21961 tgaccgacat tcgccgtatc accgacgttt gttcgctgcc gctgctggtg gatgcggata
    22021 tcggttttgg ttcttcggcc tttaacgtgg cgcgcaccgt gaaatcgatg attaaagctg
    22081 gtgcggcagg attgcatatt gaagatcagg ttggtgcgaa acgctgcggt catcgtccga
    22141 ataaagcgat cgtctcgaaa gaggagatgg tggatcggat ccgcgcggcg gtggatgcga
    22201 aaaccgatcc tgattttgtg atcatggcgc gcaccgatgc gctggcggta gaggggctgg
    22261 atgcggcgat cgagcgtgcg caggcctatg ttgaagcggg tgccgagatg ttgttcccgg
    22321 aggcgattac cgaactcgcc atgtaccgcc agtttgccga tgcggtgcag gtgccaatcc
    22381 tcgccaacat taccgaattt ggcgccacgc cgctgtttac caccgacgaa ttacgcagcg
    22441 ctcatgtcgc gatggcgctg tacccgcttt cagcgtttcg cgccatgaac cgcgccgccg
    22501 aacatgtcta caacgtcctg cgccaggaag gcacgcagaa aagcgtcatc gacaccatgc
    22561 agacccgcaa cgagctgtac gaaagcatca actactacca gtacgaagag aagctcgacg
    22621 acctgtttgc ccgtagccag gcgaaataaa aaacgcccgt tggttgtatt tgacaaccga
    22681 tgcctgatgc gccgctgacg cgacttatca ggcctacggg gtgaactgta ggtcggataa
    22741 gacgcatggc gtcgcatccg acaacaatct cgaccctaca aatgataaca atgacgagga
    22801 caatatgagc gacacaacga tcctgcaaaa cagtacccat gtcattaaac cgaaaaaatc
    22861 tgtggcgctt tctggcgttc cggcgggcaa tacggcgctc tgcaccgtgg gtaaaagtgg
    22921 caatgacttg cattaccgtg gttacgatat tcttgatctg gcggaacatt gcgaatttga
    22981 agaagtggcg catctgctga tccacggcaa actgccgacc cgtgacgaac tcgccgccta
    23041 caaaacgaaa ctgaaagccc tgcgcggttt accggctaac gtgcgtaccg tgctggaagc
    23101 cttaccggcg gcgtcacacc cgatggatgt tatgcgcacc ggcgtttccg cgctcggctg
    23161 cacgctgcca gaaaaagagg ggcataccgt ctctggcgcg cgggatattg ccgacaaact
    23221 gctggcatca cttagctcga ttctccttta ttggtatcac tacagccata acggcgaacg
    23281 tatccagccg gaaaccgatg acgactccat cggcggtcac ttcctgcatc tgctgcatgg
    23341 cgaaaagcca tcgcaaagct gggaaaaggc gatgcatatt tcgctggtgc tgtacgccga
    23401 acacgagttt aacgcctcca cctttaccag ccgggtgatt gcgggtactg gctctgatat
    23461 gtattccgcg attattggcg cgattggcgc actgcgcggg ccgaaacacg gcggggcgaa
    23521 tgaagtgtcg ctggagatcc agcaacgcta cgaaacgcca gacgaagccg aagcagatat
    23581 ccgcaagcgc gtggaaaaca aagaagtggt cattggtttt gggcatccgg tttataccat
    23641 cgccgacccg cgccaccagg tgattaaacg tgtggcgaag cagctctcgc aggaaggcgg
    23701 ctcgctgaag atgtacaaca tcgccgatcg tctggaaacg gtgatgtggg agagcaaaaa
    23761 gatgttcccc aatctcgact ggttctccgc tgtttcctac aacatgatgg gcgttcccac
    23821 cgagatgttt acaccactgt ttgttatcgc ccgcgtcacc ggatgggcgg cgcacattat
    23881 cgaacaacgt caggacaaca aaattatccg tccttccgcc aattatgttg gaccggaaga
    23941 ccgccagttt gtcgcgctgg ataagcgcca gtaaacctct acgaataaca ataaggaaac
    24001 gtacccaatg tcagctcaaa tcaacaacat ccgcccggaa tttgatcgtg aaatcgttga
    24061 tatcgtcgat tacgtcatga actacgaaat cagctccaaa gtagcctacg acaccgcaca
    24121 ttactgcctg ctcgacacgc tcggctgcgg tcttgaagct ctcgaatacc cagcctgtaa
    24181 aaaactgctg gggccaattg tccccggcac cgtcgtacct aacggcgtgc gcgtccccgg
    24241 aactcagttc cagctcgacc ccgtccaggc ggcatttaac atcggcgcga tgatccgttg
    24301 gctcgatttc aacgatacct ggctggcggc ggagtggggg catccttccg acaaccttgg
    24361 cggcattctg gcaacggcag actggctttc gcgcaacgcg gtcgccagcg gcaaagcgcc
    24421 gttgaccatg aaacaggtgc tgaccgcaat gatcaaagcc catgaaattc agggctgcat
    24481 cgcgctggaa aactccttta accgcgtcgg cctcgaccac gttctgttag tgaaagtggc
    24541 ttccaccgcc gtggtcgccg aaatgctcgg cctgacccgc gaggaaattc tcaacgctgt
    24601 ttcgctggcg tgggtggacg gtcagtcgct gcgcacctat cgccatgcgc cgaacaccgg
    24661 tacgcgtaaa tcctgggcgg cgggcgatgc cacttcccgc gcggtacgtc tggcactgat
    24721 ggcgaaaacg ggcgaaatgg gttacccgtc agccctgact gcgccggtat ggggcttcta
    24781 cgacgtctcc tttaaaggtg aatcgttccg cttccagcgt ccgtacggtt cttacgtcat
    24841 ggagaatgtg ctgttcaaaa tctccttccc ggcggagttc cactcccaga cggcagttga
    24901 agcggcgatg acgctctatg aacagatgca ggcagcaggc aaaacggcgg cggatatcga
    24961 aaaagtgacc attcgcaccc acgaagcctg tattcgcatc atcgacaaaa aagggccgct
    25021 caataacccg gctgaccgcg accactgcat tcaatacatg gtggcgatcc cgctgttgtt
    25081 cgggcgctta acggcggcag attacgagga caacgttgcg caagataaac gcatcgacac
    25141 cctgcgcgag aagatcaatt gctttgaaga tccgtcattt accgctgact accacgaccc
    25201 ggaaaaacgc gctattgcca atgccataac ccttgagttc accgacggca cgcgctttga
    25261 agaagtggtg gtggagtacc ccattggtca tgctcgccgc cgtcaggatg gtattccgaa
    25321 gctggtggat aaattcaaaa tcaatctcgc gcgccagttc ccgactcgcc agcagcagcg
    25381 cattctggag gtttctctcg acagagctcg cctggaacag atgccggtca atgagtatct
    25441 cgacctgtac gtcatttaag taaacggcgg taaggcgtaa gttcaacagg agagcattat
    25501 gtcttttagc gaattttatc agcgttcgat taacgaaccg gagcagttct gggccgagca
    25561 ggcccggcgt attgactggc agacgccctt tacgcaaacg ctcgatcaca gcaatccgcc
    25621 gtttgcccgt tggttttgtg aaggccgaac caacttgtgt cacaacgcta tcgaccgctg
    25681 gctggagaaa cagccagagg cgctggcatt gattgccgtc tcttcggaaa cagaagaaga
    25741 gcgcaccttt acctttcgcc agctgcatga cgaagtgaac gcggtggcgt caatgctgcg
    25801 ctcactgggc gtgcagcgtg gcgatcgggt gctggtgtat atgccgatga ttgccgaagc
    25861 gcatattacc ctgctggcct gcgcgcgcat tggcgctatt cactcggtgg tgtttggggg
    25921 attcgcctcg cacagtgtgg cggcgcgaat tgatgacgct aaaccggtgc tgattgtctc
    25981 ggcagatgcc ggagcgcgcg gtggcaaaat cattccctat aaaaaattgc tcgacgatgc
    26041 gataagtcag gcgcagcatc agccgcgcca tgttttgctg gtagatcgtg ggctggcgaa
    26101 aatggcgcgc gttagcgggc gggatgtcga tttcgcgtcg ttgcgccatc agcacatcgg
    26161 cgcgcgggtg ccggtggcct ggctggaatc aaacgaaacc tcctgcattc tctacacctc
    26221 cggcacgacc ggcaaaccta aaggcgtgca gcgtgacgtc ggcggatatg cggtggcgct
    26281 ggcgacctcg atggacacca tttttggcgg caaagcgggc ggtgtgttct tttgtgcttc
    26341 ggatatcggc tgggtggtgg ggcattcgta tatcgtctac gcgccgctgc tggcggggat
    26401 ggcgactatc gtttacgaag gattgccgac ctggccggac tgcggcgtgt ggtggaaaat
    26461 cgtcgagaaa tatcaagtta gccggatgtt ctcagcgccg accgccattc gcgtgctgaa
    26521 aaaattccct accgctgaaa ttcgtaaaca cgatctctcg tcgctggaag tgctctatct
    26581 ggctggagaa ccgctggacg agccgaccgc cagttgggtg agcaatacgt tggatgtgcc
    26641 ggtcatcgac aactactggc agaccgaatc cggctggccg attatggcga ttgctcgcgg
    26701 tctggacgac aggccgacgc gtctgggaag ccccggcgtg ccgatgtatg gctataacgt
    26761 gcagttgctt aatgaagtca ccggcgaacc gtgtggcgtc aacgagaaag ggatgctggt
    26821 ggtggaaggg ccgctgccgc cggggtgtat tcagaccatc tggggcgacg acgaccgctt
    26881 tgtgaagacg tactggtcgc tgttttcccg cccggtgtac gccacctttg actggggcat
    26941 ccgcgatgct gacggttatc actttattct cgggcgcact gacgatgtga ttaacgttgc
    27001 cgggcatcgg ctggggacgc gcgagattga agagagtatc tccagtcatc cgggcgttgc
    27061 cgaagtggcg gtggttgggg tgaaagatgc gctgaaaggg caggtggcgg tggcgtttgt
    27121 cattccgaaa gagagcgaca gtctggaaga tcgtgatgtg gcgcattcgc aagagaaggc
    27181 gattatggcg ctggtggaca gccagattgg caactttggc cgcccggcgc acgtctggtt
    27241 tgtctcgcaa ttgccaaaaa cgcgatccgg aaaaatgctg cgccgcacga tccaggcgat
    27301 ttgcgaagga cgcgatcctg gggatctgac gaccattgat gatccggcgt cgttggatca
    27361 gatccgccag gcgatggaag agtaaggtat tgtcggatgc gtcgcgtggc gcatccggca
    27421 ccatgcgccg atgcctgatg cgacgctga
//
LOCUS       DAPGRN010000063        26112 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPGRN010000063 DAPGRN010000000
VERSION     DAPGRN010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.063"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            384..4196
                     /locus_tag="RHW33_002776"
     CDS             384..4196
                     /locus_tag="RHW33_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDV1646263.1"
                     /translation="MAMINLSKEATVGKALTPIAILMMLSFPVASQAAGLVIKNGTVY
                     NANGVPVVDINKPNGSGLSHNIWDNLNVDKNGVVFNNSANESSTSLAGNIQGNSNLTS
                     GSAKVILNEVTSKNPSTINGMMEVAGDKADLIIANPNGITVNGGGSINTGKLTLTTGT
                     PDIQDDKLAGYSVNGGTITLGKLDNASPTEILSRNVVVNGKVSADELNVVAGNNYVNA
                     AGQVTGSVSATGSRNGYSVDVAKLGGMYANKISLVSTEKGVGVRNLGVIAGGVNGVSI
                     DSKGNLLNSNAQIQSASTINLTTNGTLDNTTGTVTSVGTISLNTNKNTIVNTRAGNIS
                     TMGDIYVNSGTIDNTNGKLAAAGMLAVDTNNATLINSGKGSSVGIEAGLVALKTGTLN
                     NSNGQIRGGYVGLESSALNNNNGDIQTTGDIAIISNGNVDNNKGLIRSSTGHIVIGAA
                     GSVNNGSTKTADTGSSDSLGIIADTGVEIGANNINNNGGQIASNGNVSLSSYSTIDDY
                     AGKILSNSKVIIKGSSLRNDTGGISGKQGIEVAVGGSLTNNIGVISSEEGDISLLANS
                     VDNHGGFMMGQNITMESMSGVNNNTALIVASKKLKINAFGNIENRDGNSFGNAYGLYF
                     GMPQQTGGMVGKEGIELSGQNIYNNNSRFIAEDGPLTLQAQNTFDNTRALVTSGADAS
                     IQVGGTYYNNYATTWSAGNLDIDATTLQNSSSGTMIDNNATGFIASDKNLSLEVVNTL
                     TNYGWISGKGDVDVTVNNGNLYNRNTIAAEKGLDIAALNGIENWKDISAGGDLTMNTN
                     RHVTNNSNSNMVGQNIVINAVNDINNRGNIVSDADLNVTTKGNLYNYLYMVGYGDIAL
                     SANSVANNNATIEATGDLIIDSKGNVGNNRGNLHALNGVLSVKGNNLNNDNGEIRGYG
                     DVTLALTGNYDSYKGSLTSETGDVTLTANIVDNAYGLIAGENVSVDAKSTIYNNTALI
                     AANKKLVINAGGNLENRDGNNFLRNNGALFGITDNVGGIVGKEGVTLSAQNVYNNNSS
                     IIAENGPLNLLSRGTLDNTRALLSSGTDAIIRAAGTFYNNYATTYSAGNLDVYAASLN
                     NASDGRLEDNTATGVIASDKNLDLSVDNSVTNYGWISGKGDVHFNVLKGTLYNRNAIA
                     ADNALTINALNGVENFKDIVAGTALTIDTQKYVTNNSNSNMLGQTIAINAVNDINNRG
                     NIVGDYSLGVKTTGNIYNYLNMLSYGVAGVSANKVTNSGKDAVLGGFYGLALEANETD
                     NTGTIVGM"
     gene            4285..5904
                     /locus_tag="RHW33_002777"
     CDS             4285..5904
                     /locus_tag="RHW33_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDV1646264.1"
                     /translation="MQLRFNFGLLLILCCFQVAAKDSLDNLIKQQKNADESTLRAHKL
                     EKKDVYSNAQRNAFTLGDLPQEKNCFIINRIELEDNFLGGQLISEVQKAVAGRCVGSA
                     GVQKIAAALQDYYINAGYITTRISIPSQDISKGKLRFQVNAGKIEKTIVEGNDISEWM
                     LPFQRNDILNIRDIEQGLENLQRVPDVDVKINIEPGTRDGYSIVHIDTHRGKNWSVRA
                     SYNNWGDEETGRYQTTAVGYLFNPAKMGDLFYLAGTRSTTGQYENVSSYYSIPVGYWE
                     YGFFYSKSKSQQVIPLSYISLDYVGTSEYLSAKATRTVYRDKTKKFAGSAELIRRKSN
                     YTLNGEELTLQARDMGNIKLGLNYKQQLPGAFWDSTLSWQRFLTWFGGEKTPDMQYGD
                     VSPVSNIFNFEGNYTRQLKNGYYNTAFFAQYAPRELTLQDQITVGDRWSIRGFENSVG
                     LSGNDGFYIKNTLAFPLPGMKANYYAGLDFGQVYQDASYGDESLVGAAVGIDGNIKSL
                     EYNFSVSTPLKYPATLDIDRVNVNFNFSYQM"
     gene            5920..6288
                     /gene="acpS"
                     /locus_tag="RHW33_002778"
     CDS             5920..6288
                     /gene="acpS"
                     /locus_tag="RHW33_002778"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV1646265.1"
                     /translation="MRIGTDIVEIARITKAIINGEMAFYERVYTAHERLLINPAEPDY
                     ERASGFWAAKEALVKAIGCGFRDGVRFQDIEIKHDAAKAPYFVISGRLQEIFIGQGLQ
                     NNSLSISHCRTHAIATVIVY"
     gene            6302..7063
                     /locus_tag="RHW33_002779"
     CDS             6302..7063
                     /locus_tag="RHW33_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1646266.1"
                     /translation="MNMYKYNDLNEKTVLVTGASGDIGLGICEKYLEQNCDVYALYKS
                     NDVQLTALKASHPAGDKLHIVQCDLACPQSVSALCEQIEREAGKIDVLVNNAGIVKDS
                     LFASMSYEDFTQVIETNMFSIFRLTKDALMLLRAAENPAIINVASIAALIPSVGQANY
                     SASKGAILGFTRTLAAEMAPWGVRVNAVAPGMIESKMVKKVSRAVVRAVTSTIPLRRL
                     GKCEEVANTIVFLSSSASSYIVGQTIVIDGGLVMR"
     gene            7067..7615
                     /locus_tag="RHW33_002780"
     CDS             7067..7615
                     /locus_tag="RHW33_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HDV1646267.1"
                     /translation="MSKYTIPSKIFLEMGGWRQPLLMVDKIADYKYGENGFVSVVKHV
                     TYNEPYLLGHFPEDPIMPGVIISEIFGQASEYLSFLTDICDIWRERFEEELKSLRDIH
                     ARIHRPEMLEIIRTRRSQVRGVLAAQNLKFKDIAYPGDSIDVVSKLAFSDASGFKHYS
                     VTAYVGKKLISQGTIINFRETK"
     gene            7637..7918
                     /locus_tag="RHW33_002781"
     CDS             7637..7918
                     /locus_tag="RHW33_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309314.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV1646268.1"
                     /translation="MSTMNDAIEQRKEVLMTIKTEIIQRLNIQRDEAQIDDDTALFGN
                     GFKLDSVDATEIVVLLDKVFDIKISESDDPSYMRSINSLATFVIQKKND"
     gene            7954..9114
                     /locus_tag="RHW33_002782"
                     /pseudo
     CDS             7954..9114
                     /locus_tag="RHW33_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309315.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
     gene            9188..11746
                     /locus_tag="RHW33_002783"
     CDS             9188..11746
                     /locus_tag="RHW33_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HDV1646269.1"
                     /translation="MKTNRKRVVITGLGILSSLAENIQDFREALLNKKNGVADSERFA
                     KWFENARAAEVLHDIEYSDLNDDVIDSLDNAALWAYKVGKDALSQAGLMDSRDTLKET
                     GMIVGVSSAGTEAFLPLFEQRMEDFSLRKALFSGAFSSCCSSVSTLLGLQGGVELVAT
                     ACTASPNAVGMAFDYIQNGKSKTMLAVGTEPIYLPTFAGFYALNVMHPDTCTPFSGQS
                     GMSIGEGAGAIVLEEYEHALARGATIYGEILSYATSCDAFHETGPDPRASGAVQVMNK
                     AMANAGVTPEQIDYVNAHGTGTEANDRIETLAMKKVFANHDKLLVSSTKSYFGHNIGA
                     AGIVELIACLATLPDNRVLPTLNFSQARPNCDLDYVPNEFRDRKVNIFMKNNYAFGGN
                     NCCMILSMEPASIPVSTYDSKRVAISGVGAVSAIGHTLNQVLENIWAQDHHVELGSVT
                     FPDDTLEEAKELLAVLDESNQFAELFDEEYSFSDATLPETASNFKTFQVSGLEPRKHL
                     RRFDQRKATRGGTFALIALTEALEQAKRKIKRDGDELGMIMGMSRGPQETTYKYLQSL
                     KPDPRKVRTSEFPGSLMNAIPTFCGISEGIKGYTTTLATGENSALGALTYGYEIVRQN
                     LQPQVLVGGADEYFPSMSLYMDAVTQKIHMTAEASDYQIYGKDPQGYVPGEGACMLLL
                     EDPQLAAARGAEVLAEVVGYGKSCSNTYFDASLLEEKSASMALAIERALTHAGISAGD
                     IDLVCGTSNGSDDHSRIEIDAIYNTFAAQNPAVPVVNYNACFGFVASAAGLLNLAVIT
                     DCIKKQAVPAIPNTVEFFDERVNFVRQSMKLALNHVLLVGATEGGNYYAFVIKG"
     gene            11751..12974
                     /locus_tag="RHW33_002784"
     CDS             11751..12974
                     /locus_tag="RHW33_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase"
                     /protein_id="HDV1646270.1"
                     /translation="MDNAAIISGYSVWMPYAEDCSQLIDNLKQGKRVARTPWFTSNEE
                     AIKCGFKGNPSVATLKQVDDSALDLLSQLIDEALEQAMLDKHCLAGRNVRVYLTGIGP
                     RIDGLDYKSFYNYNDVEDINLTQSITNLHASKMSQDTISSHLARKYRLQYLPPNMNCT
                     SNSSLTAVHLATQGIEQGGIDLAIVLNCSKIKTQDIWFLETQSMLDSEQVQPFGENSK
                     GVAFAEGFSALLLESARHRRARQRSEGVRVQTTYTQISAGRSNDASWLSTNVHKVMQA
                     AMKQAEIALDDLAAILPHGNGSAVSDNAEAKAITMFAGERQIPVLAYKGQIGYTATGS
                     GVVDLIIGHHSLTHHQLIAPVGNDVIIDSMASLVLTDGSVTNHSKRHLLKVGVGVDGS
                     VIGVVMSNMQAGRAK"
     gene            12971..13924
                     /locus_tag="RHW33_002785"
     CDS             12971..13924
                     /locus_tag="RHW33_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV1646271.1"
                     /translation="MTEKSIYLSSWALTEPDDIFYFYQPTWLNAWEAHMYERGLYGTR
                     ANGWGLKRFGVENDIYAPERIRNPQYMPYLEAMEQVTDSALQQMPTKERMKLMKSRTA
                     FIYADSWGESGPFEKISSALHIATIDTLPKNLLKKFSITEPTCKIRGEKQAFVQALRM
                     AQDYLAWDVFDNVVICAAYRAIPVLVFSEEDVGKPPKHLFPRRADEVNLSVERVGCFI
                     LSQRESAIRLQCGEYHSGTAPQHATDIDVMAFSERRQMPSRPAAGVIDLVAHYGESGC
                     LTPALSMDYLTRHIQPGGKMRTVIADKQFGYHYFDLEYLEG"
     gene            13935..14642
                     /locus_tag="RHW33_002786"
     CDS             13935..14642
                     /locus_tag="RHW33_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1646272.1"
                     /translation="MQSTIKSLPAISLSNIYKCYGAGAGKVDALKSINLDINKGEFLA
                     LCGPSGSGKSTLLNILSGIDKPTSGTVIFLNKLLNQLPEEQLAVIRGKHLGFIFQFFN
                     LMPVLNIFDNVYFPLVLNGQFSKKEATERTLHYLDSVGLSGFGDRKPGQLSGGQQQRV
                     AIARALAHEPMVVIADEPTGNLDLATGEAILDLLLTINQQTGTTFVISTHSSELKARA
                     RRVVEIQDGVLIHDSDA"
     gene            14626..15282
                     /locus_tag="RHW33_002787"
     CDS             14626..15282
                     /locus_tag="RHW33_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646273.1"
                     /translation="MTQMHKGFLLIGVFLYAAFLAPKLYAATPDAVNQVLQKGLPAQP
                     AALATLTEALKKEYEARHNVVALVFYAYGLLRQADGYAATNDFIHASEYAKSGFFWLD
                     EAVDLQEKNQRVRYLRARVDAYLPADSGRCVVTVKDTEHMLADPAIWATTIRDHILAM
                     RYRALRHCKDTTHANALLAQIKGQNAALAQSLTQDFNVVPEWDSEELTQVLLPLMKGE
                     "
     gene            15283..16593
                     /locus_tag="RHW33_002788"
     CDS             15283..16593
                     /locus_tag="RHW33_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646274.1"
                     /translation="MSGEILAVNSVLLLILFFVFYQVLFYSRDVKFAFLNLFRHKRRS
                     FSTITAIVLGGVAIFLYGGFIDYSFWILKEQTIRTNIGHVQIYNQHYFETSNKNKSLI
                     ADYAALKKAILSDPTLATDISTLSGQLEFTGVISHYESETSSYFSALGVEPLPALKLG
                     SFDKIIAGSDLSRVKGDEITLGSGLAKTLNAKYDDWLDVMVVNTAGGQGALSLKLRGI
                     FESGIKDYDDVAMKIPLDTAQRMMGTDGVSKVLILLKEDDTAAFSAKLRQFIERNHLP
                     LVVKDWRDVSLFYQQVEGLLSGIYFFIKLIVALIVIFMIGNSMAMNIVERTREITTLR
                     AIGLKPLHVTRLFLTEGIFIGVIGAVGSLLVGGVLAWIINLYGIAMPPSPGQTIGYTA
                     FIKTSNPELIWVTVVLPILTATGASVLPALRASRLNISDAFKFS"
     gene            16602..17390
                     /locus_tag="RHW33_002789"
     CDS             16602..17390
                     /locus_tag="RHW33_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HDV1646275.1"
                     /translation="MNMAFYGKWFACLWLATSCVQAASTDNKALEIIRRADEIRSPNK
                     PFRYTLTVTEYKAGVTQPENKQVLDISMRFMKPQGNEKADARSLVRFIYPPRDKGKIM
                     LSDWYDLWFYTPELRRPMPISRQQRLIGQISNGDVIVTNFEYAYDSTLVGEVSCAEKQ
                     CYKLALVRKSADITWPKVIYYVEKDGDNRPWKAAYYSQDDQLIKEVLYQDFQPVLGKT
                     RPMKITVTDVRHGNNYSVMEYSDVRLESLPEFHFTKEYIQRGAK"
     gene            17387..18775
                     /locus_tag="RHW33_002790"
     CDS             17387..18775
                     /locus_tag="RHW33_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646276.1"
                     /translation="MKRTLKISSLLCVALPLTVQADCLSGDEVAQNSDVTLYEQVSYV
                     NKQASAWQIAGKNPYTRHNGYQEAGIGINSGCSIIDNTLDLKLNLYGINEYALKPAGK
                     FETDDSRTRALINRLSLVYSASDSVQFEAGKFAAPSGMFFLRSPSDLQTHYYTGFQST
                     RLHDPKMTSAYQASSWGAKMNLDTRDYAFSASVIPKLATIDKRYVTSGNWSANQQGNS
                     DEAYLLSYSDHRFGEHTPTVNVRLGPSPSLALSDSYHYTPQLTLNVDAAYHRSQQWRH
                     LSHRETAQVEEYQFPDSLYETKDESGVELALGGQYTSDSFSVFGVEYYFQSEGYSRAE
                     QRQQRELIDFLNTTTGYAPLDQAFDSYKYLMASEISNTANQGMLQGKHYLNAWASLPL
                     AGESTLQPSLVVNLVDGSTLLGLHYSTPLSTISNQLEAYAGGYSALGSRYSEFALFGD
                     TLGLYLGFKYYL"
     gene            18789..19730
                     /locus_tag="RHW33_002791"
     CDS             18789..19730
                     /locus_tag="RHW33_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV1646277.1"
                     /translation="MNNASQPTVLLFAGQGNPVIGMGSDLWDLNASTKHIWDCASDIS
                     GLNLRRLCQKGPMNRLIQTTVQQLAVTAINVTLYTLCRERLENLTVVGSCGHSVGEYS
                     ALYAAGAISLETLFGMIHFRASLMHDESQRNKGAMLAVKGINREQLQTMITESGIALD
                     ISCDNTPRQQVIGGTQAALNEFATLLMAAGFEPVKLGVSGAWHTRLMEDGVQAMRDYL
                     AGLDIASPEHQVLMNVTAKSEVAPSIIKENLSLHLTHTVKWTESLDTFLNMPTSVAFL
                     EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
     gene            19727..20713
                     /locus_tag="RHW33_002792"
     CDS             19727..20713
                     /locus_tag="RHW33_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646278.1"
                     /translation="MIPDYLTFIRFQDKRSLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQRLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVIEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSRRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEVMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFYCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(21086..21173)
                     /locus_tag="RHW33_002793"
     tRNA            complement(21086..21173)
                     /locus_tag="RHW33_002793"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21137..21139),aa:Ser,seq:gga)
     gene            21407..22345
                     /gene="ghrA"
                     /locus_tag="RHW33_002794"
     CDS             21407..22345
                     /gene="ghrA"
                     /locus_tag="RHW33_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV1646279.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRMTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            22400..22804
                     /gene="ycdX"
                     /locus_tag="RHW33_002795"
                     /pseudo
     CDS             22400..22804
                     /gene="ycdX"
                     /locus_tag="RHW33_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
     gene            complement(22888..23193)
                     /locus_tag="RHW33_002796"
     CDS             complement(22888..23193)
                     /locus_tag="RHW33_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646280.1"
                     /translation="MKTKDIKIDFETVDDVREHRIDSTGCIFQLTNSPIGLIIYVAED
                     DVYAMWDGQDEFKGGNSHLINTHEGFDPTPVCMLAYHVTLDELPEASARIRKMKDIK"
     gene            23489..24043
                     /locus_tag="RHW33_002797"
     CDS             23489..24043
                     /locus_tag="RHW33_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646281.1"
                     /translation="MKEELTLTEISRLYGYTLNGGGKRWLERGLPMNTHTRRVPAKEG
                     CDWVLKNVINPLKQTSIKEAIDQERLRRERALADAAERENQEAMNLLIPVGYVEQQLA
                     EYCGKVKTTILQICTIDVLEILESATDQKTLKNKLREIIERRLNEVGDLFENADLGED
                     EEEELPLMDEPEEEPEEDDEFDVS"
     gene            24140..24424
                     /locus_tag="RHW33_002798"
     CDS             24140..24424
                     /locus_tag="RHW33_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646282.1"
                     /translation="MATFITYAVIITILAVISAYKLGTEPKERGELFLGAFVAFASLA
                     LIVEGISYIVGLVSMTLFNFHPTIAMHLMLTVLWVYCLIEFLTFVFKQTK"
     gene            complement(24707..25648)
                     /locus_tag="RHW33_002799"
     CDS             complement(24707..25648)
                     /locus_tag="RHW33_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646283.1"
                     /translation="MLFSANFFTNEVTNAKAVIKFGTEANKKIQPKVVAHVSFAQQKS
                     AFAGFKAHREALIAAGFGVDGKLPNGIRKAIDRVKPAKDVAVDHIVTFTVKNSSELFH
                     LVIAGDTAIVSAPNMDFQDTADCQITFGRRKEVTGKAKLNFTKSGNPYFAVFFNSHAT
                     EKEVVELVAYEGEEKERIESCEAFLELEAQVNAVSPVVEDRFAALEAQIAELKAALEE
                     KDQIIEAQKAEIEELKAKEVAPVAVVENAADEAANDEVEVKIDVMRDPMAALQLLRSF
                     KGKSHFSSDVEDEEEEQKRNAAMLHDEMYGTNFSYCQ"
     gene            complement(25763..25942)
                     /locus_tag="RHW33_002800"
     CDS             complement(25763..25942)
                     /locus_tag="RHW33_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646284.1"
                     /translation="MARPVKFTREMIVDIAKRFYGDTERGGENFFKKEGIDRSGFYKL
                     MKKYEIQVKINVQVC"
BASE COUNT         6876 a         5810 c         6594 g         6832 t
ORIGIN      
        1 tgatgatgct ttgttaatca attaacaaga tttaaattaa ttgtaactca ccaactttgt
       61 tgttttattc ggatttctga aatgatgtcg tacagaaaac tacatatcat ttcagatatc
      121 ctctaaattg gatttttttc acccaaggag caaggatggg agacttttaa attattgtgt
      181 tttagtaagt taggtacttc gccgttcgat gaaaaaacaa taactcttag ttgttttttt
      241 gagtccttat ttcagcttat ttttcgtgat tttttattgc gccaggtgat aggatttgcc
      301 gcaatcattt ttgctgatat tccgtgagtc atcaaaagtg atctgcatgt ccaaaatgaa
      361 tagcatcaat aaggaaaacg ttagtggcga tgataaattt aagtaaggaa gcaacggtgg
      421 gtaaagcatt aacccctatt gctatactta tgatgttgtc ttttcctgta gcttctcaag
      481 cggcgggatt agtcataaaa aatggaacgg tatataacgc caatggtgtg ccagtcgttg
      541 acatcaacaa acctaacggt agtggtttat ctcataatat ctgggataac ctaaacgttg
      601 ataaaaatgg tgtcgttttc aataacagcg ctaatgaatc cagtacttca cttgccggaa
      661 atattcaggg aaacagtaat ctgacctccg ggtcggcgaa ggtgatcctg aatgaggtta
      721 cttccaaaaa tccttcaacc attaatggga tgatggaagt tgcaggggat aaagcggatc
      781 tgattattgc caacccgaat ggtattactg taaacggtgg cggttcaatc aatacaggta
      841 aactgacctt aaccaccggg acgccggata tccaggatga caagctggcc ggttactccg
      901 tgaacggcgg taccattacg ctcggtaaac tggataacgc cagcccgaca gaaattctgt
      961 cccgtaacgt ggtggttaac ggcaaagtgt ctgccgatga gctgaacgtt gttgctggca
     1021 ataactatgt taatgccgca ggccaggtga ccggtagcgt atccgccacg ggttcccgta
     1081 acggttacag cgtagatgtt gccaaactgg gcggaatgta tgcgaacaaa atcagtctgg
     1141 tcagcaccga gaaaggtgtg ggggttcgca acctcggcgt tattgctggg ggggttaatg
     1201 gtgtcagcat cgattccaaa ggtaacctgt taaacagtaa cgcccagatt cagtctgcaa
     1261 gcacgatcaa cctgacaaca aatggtactc tggataacac caccggtacg gtgacatctg
     1321 taggcactat ctcgcttaat accaacaaga atactatcgt gaatacccgt gcgggtaaca
     1381 tctctacgat gggcgatatc tacgttaaca gcggtacgat tgacaatact aacggcaagc
     1441 ttgcggctgc aggaatgctg gcggttgata ccaataacgc cacgctgatt aactctggta
     1501 aagggagttc tgtcgggatt gaagcggggc tcgtggcact gaaaaccgga acgctcaaca
     1561 acagcaatgg tcagattcgc ggtggctatg tgggtcttga atcctctgcg ctgaataaca
     1621 acaacggtga tatccagacc accggcgata tcgccattat cagtaacggt aatgtggata
     1681 acaacaaagg tctgatccgt tcgtccaccg ggcatatcgt tattggcgcg gcaggtagcg
     1741 taaataatgg ttcaaccaaa accgccgata ccggcagttc tgactctctg ggcattattg
     1801 cagataccgg cgtagaaatt ggtgcgaaca acatcaataa caacggcgga cagattgcgt
     1861 ctaatggcaa cgtctccctg tcaagttaca gcacgatcga cgactatgcg ggcaaaattc
     1921 tgtccaacag caaagtgatt atcaagggaa gctcactacg taacgatacc ggggggatca
     1981 gcggtaagca gggcattgaa gtcgccgttg gcggcagcct gaccaataac attggcgtga
     2041 tcagctctga agagggtgat atctccctgt tagccaactc agtggacaac cacggcggct
     2101 tcatgatggg gcagaacatc acgatggagt cgatgtctgg cgtcaataac aacaccgcgc
     2161 tgatcgtggc cagcaaaaaa ctgaagataa acgcgttcgg taatattgaa aaccgcgatg
     2221 gcaatagctt cggtaatgca tatggtctgt acttcggcat gcctcagcaa acgggtggaa
     2281 tggtcggcaa ggaaggcatc gagctttccg ggcagaatat ctataacaac aacagtcgtt
     2341 tcatcgctga ggatggtcct ctgactctgc aggcacagaa tacgttcgac aacacgcgtg
     2401 ctctggtcac cagcggggcg gatgcatcta ttcaggttgg cggaacgtat tacaacaact
     2461 acgctaccac ctggagcgcg ggtaacctgg atatcgacgc gaccacactg caaaacagca
     2521 gtagcggtac gatgatcgat aacaatgcga ccgggttcat agcatctgat aaaaacctgt
     2581 cactggaagt ggtgaatacc cttaccaact atggctggat cagcggtaaa ggtgatgttg
     2641 atgtcacggt gaataacggc aacctgtaca accgcaatac tattgcggct gaaaaggggc
     2701 tggacattgc agcgctgaac ggtattgaaa actggaagga tatttctgct ggcggcgacc
     2761 tgacgatgaa caccaatcgc catgtgacca acaactccaa cagcaatatg gtggggcaga
     2821 atatcgttat taacgcggtt aacgatatca acaaccgtgg caacattgtc agtgacgctg
     2881 acctgaacgt gacgaccaaa ggcaacctgt ataactatct ctatatggta gggtatgggg
     2941 atatcgcatt gtcggcaaat agcgtggcga acaataacgc gaccatcgaa gcgacaggcg
     3001 atctgattat cgattcgaag ggtaacgtgg gtaacaaccg cggtaatctg catgcgttga
     3061 acggcgtgtt gtctgttaaa ggcaacaatc tgaacaacga taacggtgaa attcgtggtt
     3121 atggcgatgt cacgctggca ctgacgggca actacgacag ctataagggt tcgctgacct
     3181 ctgaaacggg tgacgtgact ctgacggcga acatcgtaga caacgcctat ggtttgattg
     3241 ccggtgagaa tgtttctgtc gatgctaaat cgacgattta caacaacact gcgctgatcg
     3301 cggcgaataa aaagctggtt attaacgctg gcggcaacct cgaaaaccgc gacgggaata
     3361 acttcctgcg taataacggc gcgctgtttg ggatcaccga caacgttggc ggcatcgtag
     3421 gtaaagaagg tgtcacgctt tctgctcaga acgtctacaa caataacagc agcatcatcg
     3481 ctgaaaatgg tccgcttaat ctgctgtcca ggggaacgct ggataatacc cgcgcgcttc
     3541 ttagcagtgg gactgatgcc atcatccgtg cggcagggac gttctacaac aactatgcca
     3601 ccacgtacag cgccggtaat ctcgacgttt atgcggcgtc gttgaacaac gccagcgatg
     3661 gtcgcctgga agacaatacc gccacgggcg tgattgcgtc tgacaaaaac ctggatctga
     3721 gcgttgataa cagtgtcact aactatggtt ggatcagcgg taaaggagat gtgcatttca
     3781 atgttctgaa aggcacgctg tataaccgta atgccatcgc ggcggacaac gcgctgacca
     3841 ttaatgccct gaacggtgtt gagaacttta aagacattgt ggcgggtact gcgctgacta
     3901 ttgatacgca gaagtatgtt accaacaaca gcaacagtaa tatgttggga caaaccatcg
     3961 cgatcaatgc cgtgaatgac attaataacc gtggaaatat tgtgggtgat tattctctgg
     4021 gtgttaaaac caccggtaat atttataact atctcaatat gctgagttat ggtgtcgctg
     4081 gcgtatcggc aaataaggtt acgaatagcg gtaaagacgc tgttctcggt ggcttctacg
     4141 gtttagcgtt agaagcaaac gaaactgata acaccggtac tattgtcggc atgtaagatg
     4201 taaccaaaga cagcagtgac ttttaatatt aatgcgtcat gttacgcagg ggttgctgct
     4261 gtctttattt agtttgagaa gataatgcaa ttacgattta attttggttt gctgctcatt
     4321 ctttgttgtt ttcaggttgc agcgaaagac agtcttgata atttgatcaa gcagcaaaaa
     4381 aatgcagatg aaagcacgtt gcgcgcacat aaattagaaa aaaaagatgt ttactcaaat
     4441 gcacaacgta atgcgtttac tctgggtgat ctgccgcagg aaaagaattg ctttatcatt
     4501 aatcggattg aactggaaga taactttctg ggtggtcagt tgatttctga agtgcaaaaa
     4561 gcggttgccg gacgttgtgt cggtagtgct ggtgtgcaaa aaattgctgc tgccttgcag
     4621 gattattata ttaatgccgg atatatcaca acgcgaatta gtatcccttc gcaggatatt
     4681 tctaaaggca agctacgttt ccaggttaat gccgggaaga tagaaaaaac gattgtcgaa
     4741 ggtaatgata tcagtgaatg gatgttacct tttcagcgta acgatattct caatattcgt
     4801 gatattgaac aggggctgga aaatcttcag cgagtacctg acgttgatgt gaaaattaat
     4861 attgaaccgg gaacacggga tggttacagc atcgttcata ttgatacaca ccgggggaaa
     4921 aactggagcg ttcgggcaag ttacaataat tggggcgatg aagaaacggg ccgttaccag
     4981 acaaccgcgg tgggttatct gtttaatcca gcgaaaatgg gggatctgtt ttatctcgcc
     5041 ggtacgcgta gtactaccgg acagtatgaa aacgtcagta gttattattc cattccggtg
     5101 gggtactggg agtacggctt tttctatagc aaaagtaaat cgcagcaggt gattccgctt
     5161 tcgtacatct cgctggatta tgttggcacc agcgaatact tgagcgcaaa ggcgacgcgt
     5221 accgtttatc gcgataaaac aaaaaaattc gccggtagtg cagaacttat tcgtcgaaag
     5281 tcgaattaca cgcttaatgg tgaggagctg acattacagg cgcgggatat gggaaatatc
     5341 aagctggggc taaattataa gcaacagtta cccggcgcgt tctgggattc aaccctctcg
     5401 tggcaacgct ttttaacctg gttcggtggt gaaaaaacgc cggatatgca atatggcgac
     5461 gttagcccgg tgagcaatat ctttaatttt gagggtaact atacccgaca actaaagaat
     5521 gggtattaca atactgcttt ttttgcccag tacgcaccgc gtgaactgac tttgcaagat
     5581 caaattacag ttggcgatcg ttggagtatt cgcgggtttg aaaatagcgt aggtttgtca
     5641 gggaatgatg gtttttatat aaaaaatacg ctggcatttc ctttgcctgg aatgaaagct
     5701 aactattatg ccggtcttga ttttgggcaa gtgtatcagg acgcttctta tggcgatgaa
     5761 tcactggtgg gcgcggcagt tggtattgat ggaaatatta aaagtcttga gtataacttt
     5821 tctgtttcta ccccattaaa gtatccggcg acattagata ttgatcgggt gaatgtaaat
     5881 tttaattttt cgtaccaaat gtaaaggata acgagactca tgagaatagg gacggatatt
     5941 gttgaaattg ccagaataac caaagccatt attaatggag agatggcttt ttatgagcgt
     6001 gtttatactg ctcatgagcg cttactcatt aaccctgcag aacctgatta tgaacgggcc
     6061 tctggattct gggcggcgaa agaagcgtta gttaaagcga ttggttgtgg ttttcgtgat
     6121 ggcgtacgtt tccaggatat tgaaataaag catgatgcgg ccaaagcacc ttatttcgtt
     6181 atttccggaa gactacaaga aatttttatc gggcaggggt tgcaaaataa ctccctgagt
     6241 atttcgcatt gccgtaccca cgccattgcg actgtcatcg tttattaact caacatatgt
     6301 gatgaatatg tacaaatata atgatttaaa tgaaaaaacc gtactggtga caggagccag
     6361 tggtgatatt ggactgggta tttgcgaaaa atatctggag cagaactgcg atgtttatgc
     6421 gctctataaa tccaacgacg tgcaattaac ggcgctgaaa gcaagccatc ctgcaggcga
     6481 taaactgcat attgtgcagt gcgatctcgc ctgcccccag agcgtgagcg cattgtgcga
     6541 acagatagag cgcgaagctg gcaagattga tgtgctggtg aataacgcgg gcatcgttaa
     6601 agacagcctg tttgcctcga tgagctatga ggatttcacc caggttatcg aaaccaacat
     6661 gttcagcatt ttccgtctga cgaaagacgc gctgatgttg cttcgtgcgg cagaaaatcc
     6721 cgccattatt aacgttgcct ctattgcagc gctgatccca agcgtcggtc aggcgaatta
     6781 cagcgcgtca aaaggggcga tcctaggctt cacccgcacg ctggcagcag aaatggcacc
     6841 ctggggcgtg cgcgtcaacg cggtcgcacc gggaatgatt gaatcgaaaa tggtgaaaaa
     6901 agtatcccgc gcagtggtcc gtgcggtaac cagtaccatc ccgttacgcc gtctggggaa
     6961 atgtgaagaa gtggcgaaca ccatcgtctt cttaagttcc tctgcctcca gctacattgt
     7021 cggccagacc atcgttatcg atggcggcct ggtcatgcgg tgagttatgt ctaaatacac
     7081 gattccttcg aaaatcttcc tcgaaatggg cggctggcgt cagccgctgc tgatggtcga
     7141 taaaattgca gattataaat acggtgaaaa tggctttgtc agtgtcgtga aacacgtcac
     7201 ctataacgaa ccgtatctac tggggcattt cccggaagat ccgattatgc ccggggtgat
     7261 tatttcggaa atcttcggcc aggccagcga gtatctctct ttcctgaccg acatttgcga
     7321 tatctggcgc gaacgttttg aggaagagct gaagtcgtta cgtgatattc acgcacgcat
     7381 tcaccgccct gaaatgctgg agattatccg tacccggcgc agccaggtac gcggcgtgct
     7441 ggcggctcag aacctgaaat ttaaagatat cgcctatccg ggtgattcta ttgatgttgt
     7501 cagcaaattg gctttttcgg atgccagcgg ctttaaacac tattccgtga ccgcgtacgt
     7561 cggtaaaaag ctgattagtc agggcaccat tatcaatttc cgtgaaacca agtaatccaa
     7621 taagagagta aatattatgt ctaccatgaa tgacgctatt gaacagcgta aagaagttct
     7681 gatgaccatt aaaactgaga tcatccagcg actgaatatt caacgcgatg aagcgcaaat
     7741 tgatgatgat actgcgctgt ttggtaatgg ttttaaactg gactccgtcg atgcaacgga
     7801 aattgtcgtt ctgctggata aagtatttga tatcaaaatc agcgaaagtg acgacccgtc
     7861 ctatatgcgc agtatcaata gtctggccac cttcgttatt caaaagaaaa acgattaact
     7921 acttattaaa gaattaatta tcaggacact tttatgaaat ctcttactga atttcacttg
     7981 aaaaataatg cggtaatggg ggtatataac aaccgtaccc taccttcttc ttatcacgat
     8041 gctatgaccg aatataaagc cgttcgtgaa aacgcgctgc tggtggacta ttctcacctc
     8101 tctattgtta gcgtcatggg cgatgatgcc tgggcgctga tcaaccagct ggtttccgca
     8161 gatgtctcca tcattcgtga tgagcaggca atctactcac tggtgttgaa tgaagagggc
     8221 accattcgcg gagacgtcta tgtgctgtgc agcatcgacg gttattacct gctgtctgaa
     8281 gatatttcgg ctaccgaact gatcgccagc atgaatacca ttctcgaaaa agcggaagaa
     8341 ctggatattc aatcgctgcc tgaaattcag gatatgcggg aaaacaactg gggggcaatc
     8401 ctgctcgaag gcccatactc ctgggagatc atgtctgaaa ttcatggctt tgacgtgatt
     8461 ggcctgcctt actacgaata catgaatacc gatgaggatc tgctgatctt ccgctgtggt
     8521 aaacacggtg agtatgcgta catgaccatc ggtgagcagg cgaaactggc ggagcagtgg
     8581 gaaaaactgt taaccgtcgg cgagaaatac ctgatgcaga ccggcggcct ggattatcaa
     8641 aaaatcgtac gcctcgaaaa tccatgctgg gatgccagcc tgtgggaagg tcaggcggtg
     8701 aatccggttc agttgcagat gcagtgggcg gtgcagtacg acaaagagga ttttattggc
     8761 aaggacgcgg tgacagagct ctcctaggag tataccggta ataaactcat cggcatgatc
     8821 gcacaggaag aatgcgaagg cattgaggct ggcgatcgcg tgctggtgga aggtcaagac
     8881 gtgggttatg tcgtgaaagc ggtattttcc ccggcattgc agcgtttcat tgccctgact
     8941 ctgctggaaa aggattacgc ctggtctgat atcagcggct acgaaattca aactgcccac
     9001 ggaattattc cagcgcaatc aaaatgtatg ccgtttattt ataacctcag catgttggtt
     9061 agcccaactg agcacagtta tatcgacgcg tcaaaaaata aaagcgcagc gtaatgaata
     9121 tcgccgcgtg accatagctt acgcggctta aattaacccc aattggcggg attgccaggt
     9181 attcttcatg aaaacgaaca ggaaaagagt tgttattacc gggctgggga ttttgtcctc
     9241 actggcggaa aatattcagg atttccggga agcattatta aataaaaaaa atggcgttgc
     9301 ggatagcgaa cgctttgcaa aatggtttga aaatgcgcgt gcggcagaag tgttgcatga
     9361 cattgaatac tccgatctga acgatgatgt tatcgattca ctggataacg cagcgctctg
     9421 ggcctacaaa gtgggtaaag atgcgctgag tcaggccggc ctgatggata gccgtgacac
     9481 cctcaaagaa acaggcatga tcgtcggtgt ttcgtccgcg ggaacagagg catttttgcc
     9541 gctgttcgaa cagcgcatgg aagatttctc gctgcgtaaa gcactctttt ccggcgcatt
     9601 ctcatcctgt tgttccagcg tttcaacgct gctgggctta cagggtgggg ttgaactggt
     9661 ggcgaccgcg tgtaccgcca gcccgaacgc cgtggggatg gcctttgact acatccagaa
     9721 tggtaaaagc aaaaccatgc tggcagtggg cactgagccc atctatctgc cgacctttgc
     9781 cggattctac gcactgaatg tcatgcatcc agacacctgt acgccgttct cgggtcagtc
     9841 cggtatgtcg attggtgaag gcgctggcgc tatcgtgcta gaagagtatg agcacgcctt
     9901 agcacgcggg gcgaccattt atggtgagat cctctcttac gccacttcct gcgatgcttt
     9961 ccatgaaacc gggccagatc cgcgtgccag cggagcggtg caggtcatga acaaggcaat
    10021 ggcaaatgcg ggtgtaaccc cagagcaaat cgattacgtc aacgcgcacg gtacaggtac
    10081 cgaagccaat gaccgtatcg aaacgctggc aatgaaaaag gtcttcgcta accacgataa
    10141 actgctggtg agctccacca aatcttactt tggtcacaac attggcgcag cggggattgt
    10201 ggaactgatt gcctgtctgg caacactgcc ggacaaccgc gtactgccga cgctgaattt
    10261 cagccaggca cgcccgaatt gcgatctgga ctatgtgccg aatgaatttc gcgatcgcaa
    10321 agtgaacatc ttcatgaaga ataactatgc cttcggcggc aacaattgct gcatgatcct
    10381 gagcatggag cccgcgtcca ttccggtcag tacctatgat tcgaagcgtg tggctatttc
    10441 gggcgtcggt gcagtatccg caattggcca cacgctcaac caggttctgg aaaacatctg
    10501 ggcgcaggac caccacgttg aactgggtag cgtaaccttc ccggacgata cactggaaga
    10561 agccaaagag ctgctggccg tgctggatga atcaaaccag tttgccgagc tgtttgatga
    10621 agagtacagc ttcagcgatg ctacgcttcc ggaaacggca agcaacttca aaacgttcca
    10681 ggtttccggg ctggagccgc gtaaacacct gcgtcgtttc gatcagcgta aagcaacccg
    10741 tggcggcacg tttgccctga tcgcactcac cgaagcgctg gaacaggcga agcgtaaaat
    10801 caaacgcgac ggcgatgagc tgggcatgat catggggatg tcccgcggcc cgcaggaaac
    10861 cacctacaaa tatctgcaaa gtctgaaacc cgatccacgc aaagtacgta cgtcggaatt
    10921 tcctggctcg ctgatgaatg cgatcccaac gttctgcggg atctctgaag ggatcaaagg
    10981 gtataccact acgcttgcga caggggaaaa ctcggcgctg ggcgcgctga cctacggtta
    11041 tgaaatcgtc cggcagaacc tgcaaccgca ggtattggtt ggcggcgcgg atgagtactt
    11101 cccgtctatg tcgctgtata tggatgcggt gacgcaaaag atccacatga cggctgaggc
    11161 gagcgactat caaatttacg gtaaagatcc gcagggttat gttccgggcg agggtgcctg
    11221 catgctgctg ctggaagatc cacagttggc tgcggcgcgt ggcgctgagg ttctcgcgga
    11281 agtggtgggc tacggcaagt catgtagtaa tacctacttc gacgcctcat tgttggaaga
    11341 aaaatccgcg tcgatggcgc tggccattga acgcgccctt acgcatgcgg gcatctctgc
    11401 tggcgatatt gatttggtat gtggtaccag caacggtagc gatgaccatt ctcgtatcga
    11461 aattgacgcc atttacaaca ccttcgccgc gcaaaacccg gcggtgccgg tagtgaacta
    11521 taacgcttgc tttggttttg tcgcctctgc cgctggtttg cttaacctcg ccgtgattac
    11581 tgactgcatt aagaagcagg cagtacctgc gattccaaat acagtggaat tctttgatga
    11641 gcgtgtgaat tttgtacgcc agtcgatgaa acttgcgctg aaccatgtcc tgcttgttgg
    11701 cgcaacggaa ggcggcaact actacgcgtt cgtcattaag ggataagact gtggacaatg
    11761 ccgctattat ttcgggttac agcgtgtgga tgccgtatgc cgaagactgt tcgcagttaa
    11821 ttgacaatct gaaacagggg aaacgcgttg cgcgtacacc ctggtttacc tccaacgagg
    11881 aggccattaa atgcgggttt aagggtaatc cgtcagtggc gacgttgaaa caggtggacg
    11941 atagcgcatt ggacctgctg tctcagttga tcgacgaagc cctggaacag gcaatgctgg
    12001 ataagcattg cctggccggg cgcaacgttc gcgtttacct gaccggtatt gggccgcgta
    12061 tcgacgggct ggattacaag tctttctata attacaacga tgtagaagat atcaacttaa
    12121 cgcaatccat cacaaatctg catgcctcaa agatgtcgca ggacacgatt tcaagccatc
    12181 ttgcgcgcaa atatcgcttg cagtatttgc cgcctaacat gaactgcacc agtaactcgt
    12241 cactgactgc ggtgcatctg gcgacgcagg gcattgagca gggcgggatt gatctcgcga
    12301 ttgtgctgaa ctgttcgaaa attaaaaccc aggacatctg gttcctggaa actcaatcga
    12361 tgctggacag cgaacaagtg cagccgtttg gtgaaaacag taaaggtgtg gcgttcgcag
    12421 aagggtttag cgcgcttctg ctggaaagcg ctcgtcaccg tcgggcgcga caacgaagtg
    12481 agggcgtaag ggtgcaaacg acttacaccc aaatcagcgc gggccgcagc aacgatgcct
    12541 cctggcttag cactaacgtg cacaaggtca tgcaggccgc gatgaagcag gctgaaattg
    12601 cgctggatga tcttgcagcc attcttccac acggcaacgg ttcggcagtg agcgataacg
    12661 cggaagccaa agccatcacc atgttcgcag gggagcggca gatccccgtt ctcgcctata
    12721 aagggcagat cggctatacc gcgaccggat ctggtgttgt cgatctgatc attggccacc
    12781 attcgttgac gcatcatcaa ctgatcgcac ctgttggcaa cgacgtcatt atcgacagca
    12841 tggcttcgct ggtacttacg gacggcagcg tgacaaacca tagcaaacgc catttgctga
    12901 aggttggtgt gggtgtcgat ggttcggtta ttggcgttgt tatgtcaaat atgcaagcgg
    12961 ggcgcgcgaa atgacggaaa agagtatcta cctgtcttcc tgggcgctta cggaaccgga
    13021 cgacattttt tatttttatc agccgacctg gctcaacgcc tgggaagcac atatgtacga
    13081 aaggggatta tacggcaccc gtgctaatgg ctggggactg aagcgctttg gcgtggagaa
    13141 cgatatttat gcgccagaac ggatccgtaa tccacaatat atgccttacc ttgaggcgat
    13201 ggagcaggta accgattctg cattacaaca aatgccgact aaagagcgca tgaagttaat
    13261 gaaatcgcgc acggcattta tttatgccga ttcctggggg gaatccggtc cgtttgaaaa
    13321 aatttccagt gcgctgcata tcgccactat tgatacgctt cccaagaatt tacttaaaaa
    13381 gttttcgata acggaaccga cttgcaagat tcgcggcgaa aaacaggcat tcgttcaggc
    13441 gctgcgtatg gcgcaggatt atctcgcctg ggatgtattc gataacgtgg tgatttgcgc
    13501 tgcctatcgg gcaattccgg tactggtatt ctctgaagag gatgtgggta aaccgcctaa
    13561 gcatttattc ccccgtcgcg ctgatgaggt gaatttatct gttgaacgcg tgggttgttt
    13621 tattctcagc cagcgtgaga gcgccatcag gctgcaatgc ggggaatatc attccggcac
    13681 tgcgccacag catgctacgg atattgatgt catggccttc agcgaacggc gacaaatgcc
    13741 gtcccggcct gctgctggcg ttatcgacct ggtggcccat tacggtgaaa gcggttgtct
    13801 gacgcccgcg ttgagtatgg attatttaac ccgtcatatt cagcccggtg gaaaaatgcg
    13861 tacggtcatt gccgataaac agtttggtta tcactacttt gaccttgaat accttgaagg
    13921 ataacgacag tcgtatgcaa tctactatta aatcgttgcc cgctatttcg cttagcaata
    13981 tttataagtg ttatggtgcc ggggcaggaa aggttgatgc gcttaaaagc attaatcttg
    14041 atatcaataa aggcgaattt ctcgcactgt gtgggccgtc aggcagtggt aaaagtactc
    14101 tgttgaatat tctctcgggt attgataaac cgaccagcgg aacggtgata tttttaaata
    14161 aattactgaa ccaactgccg gaagagcagt tggctgtgat tcgtggaaaa catttgggtt
    14221 ttatttttca gttttttaac cttatgccgg tattaaatat tttcgataac gtttatttcc
    14281 cactggtgct taatggtcag ttcagtaaaa aagaagccac tgaacggacg ctgcattatc
    14341 tcgatagtgt tggattaagc ggttttggcg atcgtaaacc tgggcaacta tcgggcggtc
    14401 aacaacagcg tgtcgccatc gccagggcac tggcccacga accgatggtg gtcattgccg
    14461 atgaaccgac gggaaattta gacctggcaa ccggcgaggc tattttggac ctgctgttga
    14521 cgattaatca gcagacaggc accacatttg ttatttccac gcactccagc gaattaaaag
    14581 cgcgggcacg gcgagtggtt gaaattcagg atggagtatt gattcatgac tcagatgcat
    14641 aaaggattct tgctcatcgg cgtctttctg tatgcggcgt ttttggcccc gaagctttac
    14701 gcggcaacac ccgacgccgt taaccaggtt ctgcaaaagg ggcttccggc gcaacctgcc
    14761 gcgctggcga cgctgaccga ggcgttaaag aaagagtatg aagccaggca caatgtggtg
    14821 gcgctggttt tttacgccta tggcttactg cgtcaggcgg acggttatgc ggcaacaaac
    14881 gattttatcc atgcctcaga atacgccaaa agcggctttt tctggctgga tgaagcggtg
    14941 gatttgcagg agaaaaatca acgcgtacgc tatttacgcg cccgcgtaga tgcctattta
    15001 ccggcagatt cagggcgttg cgtggtgaca gtcaaagaca ccgaacacat gctggctgac
    15061 ccggcgatat gggcgacaac gatccgcgac catattctgg cgatgcgtta tcgcgcatta
    15121 cgtcactgca aggacaccac ccacgcgaat gcgttgttgg cgcaaattaa agggcaaaac
    15181 gccgcgcttg cgcagtcgct gacgcaggat tttaatgttg taccagaatg ggatagtgaa
    15241 gagttaaccc aggtactgct gccgctgatg aaaggtgagt aaatgtccgg ggaaatttta
    15301 gctgttaata gcgttctgct gctgatcctg ttttttgtgt tttatcaggt tctgttttat
    15361 tcacgagatg tcaaatttgc atttttgaat ctctttcgcc acaagcgtcg ctctttttct
    15421 accataaccg ctattgtatt gggtggtgtg gcgatctttt tatatggcgg atttattgat
    15481 tactctttct ggatcctgaa agagcagacg attcgtacca atattggtca tgtgcagatt
    15541 tacaatcagc actattttga aacgtcgaat aaaaataaga gcctgattgc cgattatgcg
    15601 gcgttaaaga aagctatttt gtcggacccg acgctggcga cggatatttc cacgctctcc
    15661 gggcaactgg agtttactgg cgtaatttca cattacgaga gcgaaacctc cagctatttc
    15721 tctgcgctgg gtgtcgagcc attgcccgct ttaaagctgg gatcgttcga taagattatc
    15781 gcggggagcg acctttcccg ggtaaaaggt gatgaaatca ccctgggcag cggcctggcg
    15841 aaaacgttga atgcgaaata cgacgactgg ctggatgtga tggtggtcaa cacggcgggc
    15901 gggcagggcg cattgtcgct aaaactgcgt ggcatttttg agtcaggcat taaagattat
    15961 gacgatgtcg cgatgaagat cccgctggac accgcgcagc gcatgatggg tacggatggc
    16021 gtcagcaaag tgctaattct gcttaaagaa gatgacaccg cggcgtttag cgcgaagctg
    16081 cgtcagttta tcgaacgcaa tcatttgccg ctggtggtca aagactggcg tgatgtttca
    16141 ctcttttatc aacaggttga agggttgcta tcggggatct atttcttcat caagctgatt
    16201 gttgcgctga ttgtcatctt catgatcggc aactcgatgg caatgaacat tgttgagcgc
    16261 acccgtgaaa ttaccactct gcgtgccata ggcctgaagc cgcttcacgt cacgcgcttg
    16321 tttctgacag aaggcatttt tattggtgtg attggcgcag tcggcagcct gctggtgggg
    16381 ggcgtgttag cctggattat taatctttat ggtattgcga tgccgccatc gccgggacaa
    16441 accatcggct ataccgcctt tataaaaacc agtaacccgg aattgatttg ggtgacggtg
    16501 gtattaccta ttttgacagc aacaggtgcc tctgtgttac cggctttacg tgcttcccgg
    16561 ctgaatattt ctgacgcatt taaattctcg taggactgat tatgaatatg gctttttacg
    16621 gcaagtggtt tgcttgcctg tggctcgcta cgtcgtgcgt tcaggcggcg agcacagata
    16681 ataaggcgct ggagataatt cgtcgcgctg acgaaattcg ttcgccgaat aaaccgtttc
    16741 gttacacgct gacggtaacg gaatataaag cgggcgtaac ccagccagag aataaacagg
    16801 tcctcgatat ttccatgcgc tttatgaaac cgcagggtaa tgaaaaagcg gatgcacgct
    16861 cgctggtgcg ttttatttat ccgccgcgag ataaaggaaa aatcatgctt tccgactggt
    16921 acgatttatg gttttacacg ccggaactgc gacgtcctat gcctatttca cggcaacaaa
    16981 gattaattgg gcaaatctct aacggcgatg tcattgtcac taactttgaa tatgcttatg
    17041 actcgaccct ggtgggggaa gtctcttgtg ctgaaaagca gtgttacaag ctcgcgctgg
    17101 tgcgtaaatc ggcggatatc acctggccga aggttatcta ttacgttgaa aaagacggtg
    17161 ataatcgccc gtggaaggcg gcctattatt cgcaggatga ccagttaata aaagaagtgt
    17221 tgtatcagga tttccagccg gtgttgggga aaacgcgacc catgaaaata accgttaccg
    17281 atgttcgtca cggcaataac tattcagtga tggagtacag cgatgttcgt ctggaatcgc
    17341 tgccggagtt tcatttcacc aaagagtata ttcagcgagg tgcgaaatga aacgtacgct
    17401 gaagatctct tcgttgctgt gtgttgcgct gccgttgacg gtacaggcgg attgcctgtc
    17461 aggtgacgag gtggcacaaa acagcgatgt gacgctttac gagcaggtgt cttatgtcaa
    17521 taaacaggct tcggcctggc agattgcagg caaaaatccc tacacacgtc acaacggtta
    17581 tcaggaggcg gggattggca tcaatagtgg ttgttcaata atcgacaata cgctggacct
    17641 taagctcaat ctgtatggaa taaacgaata tgcgcttaaa cccgcaggga aattcgaaac
    17701 ggatgatagc cgcacgcgtg cgttaattaa ccgcctcagt ctggtctata gcgcctcgga
    17761 cagcgttcaa tttgaagcag gcaaatttgc cgcgccgtca ggaatgttct ttttgcgttc
    17821 gccttctgat ttgcaaacgc attattacac cggattccag tcgacacgtt tgcacgatcc
    17881 caaaatgaca tcggcgtatc aggcgtcatc gtggggcgca aaaatgaact tggatacacg
    17941 cgattatgcg ttttcggcga gtgtcatacc gaagttagcc accatcgaca aacgctatgt
    18001 gacgtctggc aactggtctg ctaatcagca gggaaacagc gacgaagcct atctgctgag
    18061 ctacagcgat catcgctttg gtgagcacac accgacagtc aatgtgcggc tcggaccatc
    18121 gccgtcactc gcgctgtctg acagttatca ctacaccccg cagcttacgc tgaatgtcga
    18181 cgcagcctac caccgctccc agcaatggcg gcatctttcc caccgtgaga cggcgcaggt
    18241 cgaggaatat cagtttccgg attcgctcta tgaaacgaaa gacgaaagcg gcgtggagct
    18301 ggccctgggg gggcagtaca ccagcgatag tttcagcgtg tttggcgttg agtattactt
    18361 tcagagcgag ggttattcgc gtgctgaaca gcgtcagcag cgcgagttaa ttgatttcct
    18421 caacaccaca accggttatg cgccactgga tcaggcattt gattcttaca aatacctgat
    18481 ggcttcagaa atcagcaata ccgccaatca ggggatgctg cagggtaagc actatctgaa
    18541 cgcctgggcc agtctgccgc tggcgggaga gtcgacgctg cagccttcgc tggtggtgaa
    18601 tctcgttgat ggcagcacgt tgctgggcct gcattattcc acgccgctca gcactatcag
    18661 taaccagctt gaagcctatg ccggtggcta tagcgcgctg ggaagtcggt attctgaatt
    18721 tgcgcttttt ggcgacacgt taggccttta tttgggcttt aaatactatc tataaggata
    18781 aagagatgat gaataatgct tcacaaccga cagtactgct ttttgccggt cagggtaatc
    18841 cggttattgg catgggcagc gatctttggg atctcaatgc cagtacaaaa catatctggg
    18901 attgtgccag cgatatttcc ggactgaacc tgcgtcgcct gtgtcagaaa gggccgatga
    18961 atcgccttat tcaaacgacg gtacaacaac tggcggtgac cgcaattaat gtaacgctct
    19021 ataccctgtg ccgcgagcgt cttgagaatt taacggttgt gggctcctgc ggacacagcg
    19081 ttggtgaata cagtgcgctc tacgctgccg gagctatttc tcttgagacg ctatttggca
    19141 tgattcactt cagagccagc ctgatgcatg acgaaagcca gagaaataaa ggcgcaatgc
    19201 tggcggttaa aggcatcaac cgcgaacaac tgcagacgat gattacggaa tcaggcattg
    19261 cgctggatat tagctgtgat aacacgccgc gccagcaggt tattggggga acgcaggctg
    19321 cgttgaatga atttgccacg ctgctgatgg ccgctggatt cgaaccggtt aaactcggtg
    19381 tgagcggtgc ctggcacacg cgtttgatgg aagacggcgt tcaggcaatg cgcgattatc
    19441 ttgccggact ggatatcgct tcgccagagc atcaggttct gatgaacgta acagcaaaaa
    19501 gcgaggtcgc tccgtcaatt atcaaagaaa atctctcatt acacttaact catacggtga
    19561 aatggactga atctctcgat acatttctga atatgccaac ctcggtagct ttccttgaaa
    19621 taagtaataa gccctatttg gggaatatgt taaacgattt cgctggcgtg gatcagcaac
    19681 gagttatgca ttgtcgtaaa gcatttagtg atgcgaaggt attcaaatga ttcctgatta
    19741 tttaactttt attcgctttc aggataaacg aagtctgata tacatttatg ctattggact
    19801 tattctgata ggcttttatt ggaaaaatgc aggatttact tttccatcag aggatattgg
    19861 tgtcgttagt gggattctgg ccttggtgct gtataatttt atttttgatc tcaaggcgta
    19921 ctgggcttat aaatgcgtca cgaagaatat cgatttttcg tggtttaaga aaaagcagaa
    19981 ccacaaaata gaattatttc ttacgcaacc tctggtggca ggatttctgt cgttaatcat
    20041 gttgagtgca atgagttggg ggctatacca gcgtctaccc tcgttatatg cgctgttcct
    20101 gatttcgtta cttgggccgt tggttatctt tctgctgttt cggatgatcc gcaccagtta
    20161 tgtcaagcag gtcgctattt cagtagcgaa aaaagtaaaa tataaaagtc tgactcgcta
    20221 tgtgctgctt tcggtgtgca tctcaacggt tgttaacctg cttactatca gcccgttgcg
    20281 taacagtgat tcttttgtga tagaggggca gtggttaacg tttaaatcga taattgcatt
    20341 gctcattctt tgtggcgtag tgttggcgat taatctgttt tttctgcgct tctccaggcg
    20401 gtacgctttc ctgggcaggc tttttttgca ggaaatcgat ctgtttttct ccagtgaaaa
    20461 tgcgttgtcg accttttttg ccaagccgct ttggcttcgg ttattcatat tgctggttat
    20521 tgaagtgatg tggattacgc tggtgtcggt attggcaacg cttgtggaat ggcggatttg
    20581 gtttgaagcc tattttttac tctgctatgt accgtgctta atttactatt ttttctattg
    20641 tcgatttctc tggcataacg attttatgat ggcatgtgac atgtatttcc gttgggggca
    20701 ttttaataag tgaggaagtg ataggaagtg accagataat acatatatgt tctgtactct
    20761 cttgcgcatt ttgattgttg actgagtaac cagacagttg atgtgcacga tttcccctcg
    20821 ccctaacaga cgtgggcggg ggcacccggt gtgtacaaca tacgaaaaaa agcccgtact
    20881 ttcgtacgag ctcttcttta aatatggcgg tgaggggggg attgactcgc ttcgctcgcc
    20941 ctgcgggcag cccgctcact gcgttcacgg tctgtccaac tggctgtcgc cagttgtcga
    21001 cctccggtcg gggcttctca tccctccggt gtgtgcaata tacgaaaaaa aagcccgtac
    21061 tttcgtacga gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt
    21121 tgccgtatac acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt
    21181 cgttcctgtc ttgctggaac gggcgctaat ttagggaaat catgacctga ggtcaacaaa
    21241 cttttttaaa aaatcgcgcg tttattcaaa cttcaatcaa tatgtggttt taataagcga
    21301 aatctgcttt ttttgccacc gaccacggat ttgttatgct ggtggtcttt gtagatcata
    21361 acgataagtg cgaataaatt tcgcacaacg cttttcggga gtcagtatgg atatcatctt
    21421 ttatcaccca acgttcgata cccaatggtg gattgaggca ctgcgcaaag ctattcccca
    21481 ggcaagagtc agagcatgga aaagcggaga taatgactct gctgattatg ctttagtctg
    21541 gcatcctcct gttgaaatgc tggcagggcg cgatcttaaa gcggtgttcg cactcggggc
    21601 cggtgttgat tctattttga gcaagctaca ggcacaccct gaaatgctga acccttctgt
    21661 tccacttttt cgcctggaag ataccggtat gggcgagcaa atgcaggaat atgctgtcag
    21721 tcaggtgctg cattggtttc gacgttttga cgattatcgc atccagcaaa atagttcgca
    21781 ttggcaaccg ctgcctgaat atcatcggga agattttacc atcggcattt tgggcgcagg
    21841 cgtactgggc agtaaagttg ctcagagtct gcaaacctgg cgctttccgc tgcgttgctg
    21901 gagtcgaacc cgtaaatcgt ggcctggcgt gcaaagcttt gccggacggg aagaactgtc
    21961 tgcatttctg agccaatgtc gggtattgat taatttgtta ccgaataccc ctgaaaccgt
    22021 cggcattatt aatcaacaat tactcgaaaa attaccggat ggcgcgtatc tcctcaacct
    22081 ggcgcgtggt gttcatgttg tggaagatga cctgctcgcg gcgctggata gcgggaaagt
    22141 taaaggcgca atgttggatg tttttaatcg tgagccctta ccgccggaaa gtccactctg
    22201 gcaacatcca cgcatgacga taacaccaca tgtcgccgcg attacccgtc ccgctgaagc
    22261 tgtggagtac atttctcgca ccattgccca gctcgaaaaa ggggagaggg tctgcgggca
    22321 agtcgaccgc gcacgcggct actaataaag catcaggatt cctgctatcc ttggcgggaa
    22381 ttgaatacag gagagagtta tgtatcccgt cgaccttcat atgcataccg ttgccagcac
    22441 gcatgcatat agcacattga gtgattatat tgcccaggcc aaacaaaagg gcattaaact
    22501 ttttgcgatc accgatcatg gcccggatat ggaagatgcg ccacatcact ggcacttcat
    22561 taacatgcgt atctggccgc gagtggttga tggggtaggg atcctgcgcg gcatcgaagc
    22621 taacattaaa aatgttgatg gtgaaattga ctgcagcggc aaaatgtttg attcgctgga
    22681 tctgattatt gccggttttc atgagccggt ttttgcgcca catgataaag cgaccaatac
    22741 acaagcgatg atcgccacta tcgccagcgg caatgtgcat ataataagcc atcccggaaa
    22801 cccgaacgta gtcaaccccc aataacaccc gatttccaat aaaaaagccc catcacaagg
    22861 acagggctat agaatcaatt acttacatca cttaatatct ttcattttac ggatacgcgc
    22921 gcttgcttcc ggtagttcat ccagtgttac gtgataagca agcatacaga caggagtcgg
    22981 atcgaatccc tcatgagtat tgatcagatg gctattcccg cctttaaact catcctgtcc
    23041 atcccacata gcgtacacgt catcttctgc tacatagata atcaatccaa taggggagtt
    23101 agtaagctgg aagatgcagc cagtagaatc aatacgatgt tcccgaacgt catcaacagt
    23161 ttcaaagtca atcttaatat ctttagtctt catgatttgc cctcgtgtga aaaatatttt
    23221 cgattattta taaaaagact tgacaaaatc aaaaggtact gttctacggg tgaaataaag
    23281 cgagttttcg gcaccccgtt atttgatgtg cgcgtcagga tctaaaacgt gatcacttca
    23341 ctcatggcgc ggtcgctacg cgactattaa cccacttcct aacccttacc attactggat
    23401 ctttcccttc gttagaatgc gatttaggcg cttagatttt gcgtaaaacc aataaataaa
    23461 attacgcaaa tttaatggag gataaccaat gaaagaagaa ttaaccctta cagaaatttc
    23521 ccgtttatac ggctatacat tgaatggtgg cggtaagcga tggttagaaa ggggactacc
    23581 gatgaacacc cacacgcgtc gtgtaccagc aaaggaaggt tgcgattggg tgctaaagaa
    23641 cgtaattaac ccacttaaac aaacttcaat caaagaagcg attgatcaag aacgtctccg
    23701 cagagaaaga gcattagcgg acgcagccga acgagagaac caggaagcaa tgaatctcct
    23761 gattcccgtc ggttatgtag aacagcaact agcggaatat tgcgggaaag ttaaaactac
    23821 catcttacaa atttgtacga ttgacgtttt agaaattctc gaatctgcca ctgaccagaa
    23881 aacattaaag aataagctaa gggagattat cgagcgtcgt ttaaatgaag taggggactt
    23941 gttcgaaaat gcagatttgg gagaagacga ggaagaagaa ttaccattaa tggatgaacc
    24001 ggaagaagaa ccagaagaag acgatgaatt tgatgtgtct taaaaattag atactaaata
    24061 catatgtgaa gggtgttgaa cgggtcaacg tctacggggg agaacatccc ccgcttaact
    24121 tttaagagga tataaaacta tggctacttt tattacctat gctgtcatca ttactattct
    24181 ggctgttatt tctgcttata aactcggaac cgaaccgaaa gaacgaggcg aattattctt
    24241 aggtgcgttt gttgcatttg ctagccttgc gttaattgtc gaaggaattt cttatattgt
    24301 tggcctagtt agtatgacac tttttaactt ccatccaact attgctatgc atttgatgct
    24361 gaccgtactt tgggtatatt gcttaatcga atttttaacg tttgttttca aacagactaa
    24421 ataatattgt aatctgcctt ttctccttat gtggttttaa cagggaattt tcgaaagaga
    24481 gttcccgatt aaaagccata aaacacattc agtacagtgt tttcatagtc agtgatctat
    24541 acaacataaa aacataacaa gtatagattg agtattaatc ctcccgccct tcggggcggt
    24601 tatttttctg gctttaaaga ataatgtagc attatgttca ttataacctc atctttgttg
    24661 cgcaaaccta acctaaactt actagggact cttcggagtc ccttttttat tggcagtaag
    24721 aaaagttagt accgtacatt tcatcatgaa gcattgcagc attgcgtttc tgttcttctt
    24781 cctcatcttc tacatcacta gaaaaatgag atttgccctt aaaagaacga agtaattgca
    24841 gtgcagccat cggatcacgc atcacatcga ttttcacttc tacttcatca tttgcggcct
    24901 catcagccgc gttttcaact actgccaccg gagcaacttc tttagctttc agttcttcaa
    24961 tctcagcttt ctgtgcttcg ataatctggt ctttttcttc cagtgcagct ttcagttctg
    25021 cgatctgtgc ttccagtgca gcaaagcgat cttcaacaac cggagaaaca gcattcacct
    25081 gtgcttccag ttccagaaat gcttcgcaag attcaatacg ttctttctct tcaccttcat
    25141 aagcaaccag ttctacaact tctttttcgg tagcgtggct gttaaagaaa acagcaaagt
    25201 aaggattgcc ggatttagtg aagttcagtt tagcttttcc ggtaacttct ttacgacgac
    25261 cgaaggtgat ctggcaatca gcggtatcct gaaaatccat gttaggagcg gaaacgatag
    25321 cagtatcacc agcaataacc aggtggaaca gttcagaaga gttcttaacg gtaaaagtaa
    25381 cgatgtggtc tactgcaacg tctttagcag gtttaacgcg gtcgattgct ttacgaatgc
    25441 cgttaggcag tttaccgtca acaccaaagc cagccgcgat cagtgcttca cgatgtgctt
    25501 taaagccagc gaaggcggat ttctgctgcg caaaggaaac atgagcaaca actttcggct
    25561 gaatcttctt gtttgcttcg gtgccaaatt tgataactgc tttagcgttg gttacttcgt
    25621 tagtgaaaaa gttagcggaa aataacatta taaatactcc tgtggaaagt tagttttaat
    25681 tgttcatttc aagcctcctt tgctgcaaca agggaggctt tttctttttt aaagccatca
    25741 tataattctc agtgaaagtg gattaacaaa cttgaacgtt aatctttacc tgaatttcgt
    25801 atttcttcat tagcttgtag aatccgcttc tatcaattcc ttccttctta aagaaattct
    25861 cgccgcctcg ttcagtatcg ccataaaagc gtttagcaat atcaacaatc atttcacgag
    25921 taaacttaac aggtctagcc ataagtagat tcctcaaatg tggattaata aggacaataa
    25981 aaagagcttt tcgccctcga tacttctatt tataaaagtc atttcagaaa accctgcaaa
    26041 accactgtat atctgatcag tgggagcacg aattgctaaa gagaagatta ataattaatc
    26101 aattcgtaca tt
//
LOCUS       DAPGRN010000064        26106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPGRN010000064 DAPGRN010000000
VERSION     DAPGRN010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.064"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..322
                     /gene="bhsA"
                     /locus_tag="RHW33_002801"
     CDS             65..322
                     /gene="bhsA"
                     /locus_tag="RHW33_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV1646285.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            complement(404..1366)
                     /gene="ldtC"
                     /locus_tag="RHW33_002802"
     CDS             complement(404..1366)
                     /gene="ldtC"
                     /locus_tag="RHW33_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV1646286.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVVLALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKNAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(1508..4954)
                     /gene="mfd"
                     /locus_tag="RHW33_002803"
     CDS             complement(1508..4954)
                     /gene="mfd"
                     /locus_tag="RHW33_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV1646287.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQRLPDLAVQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(5082..6152)
                     /locus_tag="RHW33_002804"
     CDS             complement(5082..6152)
                     /locus_tag="RHW33_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV1646288.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGIVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMLTASTSLWYLYALIVYFVVCKIF
                     SRLALPLFTLFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLAVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAGRWSPQVELALLLVYPFVSLLICTLAGLLVRKF
                     SQRAFSDLLFSPPSLPATSYSR"
     gene            6414..7613
                     /gene="lolC"
                     /locus_tag="RHW33_002805"
     CDS             6414..7613
                     /gene="lolC"
                     /locus_tag="RHW33_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV1646289.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            7606..8307
                     /gene="lolD"
                     /locus_tag="RHW33_002806"
     CDS             7606..8307
                     /gene="lolD"
                     /locus_tag="RHW33_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV1646290.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALDMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            8307..9551
                     /gene="lolE"
                     /locus_tag="RHW33_002807"
     CDS             8307..9551
                     /gene="lolE"
                     /locus_tag="RHW33_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV1646291.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQHLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            9580..10491
                     /gene="nagK"
                     /locus_tag="RHW33_002808"
     CDS             9580..10491
                     /gene="nagK"
                     /locus_tag="RHW33_002808"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV1646292.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            10507..11328
                     /gene="cobB"
                     /locus_tag="RHW33_002809"
     CDS             10507..11328
                     /gene="cobB"
                     /locus_tag="RHW33_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV1646293.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     LLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11484..12530)
                     /gene="potD"
                     /locus_tag="RHW33_002810"
     CDS             complement(11484..12530)
                     /gene="potD"
                     /locus_tag="RHW33_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV1646294.1"
                     /translation="MKKWSRHLLAAGVLALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12527..13321)
                     /gene="potC"
                     /locus_tag="RHW33_002811"
     CDS             complement(12527..13321)
                     /gene="potC"
                     /locus_tag="RHW33_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV1646295.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13318..14175)
                     /gene="potB"
                     /locus_tag="RHW33_002812"
     CDS             complement(13318..14175)
                     /gene="potB"
                     /locus_tag="RHW33_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV1646296.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(14159..15295)
                     /gene="potA"
                     /locus_tag="RHW33_002813"
     CDS             complement(14159..15295)
                     /gene="potA"
                     /locus_tag="RHW33_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV1646297.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15545..16771
                     /gene="pepT"
                     /locus_tag="RHW33_002814"
     CDS             15545..16771
                     /gene="pepT"
                     /locus_tag="RHW33_002814"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV1646298.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(16820..17941)
                     /gene="roxA"
                     /locus_tag="RHW33_002815"
                     /gene_synonym="ycfD"
     CDS             complement(16820..17941)
                     /gene="roxA"
                     /locus_tag="RHW33_002815"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV1646299.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(18017..19477)
                     /gene="phoQ"
                     /locus_tag="RHW33_002816"
     CDS             complement(18017..19477)
                     /gene="phoQ"
                     /locus_tag="RHW33_002816"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV1646300.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSTPKDE"
     gene            complement(19477..20148)
                     /gene="phoP"
                     /locus_tag="RHW33_002817"
     CDS             complement(19477..20148)
                     /gene="phoP"
                     /locus_tag="RHW33_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV1646301.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(20316..21686)
                     /gene="purB"
                     /locus_tag="RHW33_002818"
     CDS             complement(20316..21686)
                     /gene="purB"
                     /locus_tag="RHW33_002818"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV1646302.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(21690..22331)
                     /gene="hflD"
                     /locus_tag="RHW33_002819"
     CDS             complement(21690..22331)
                     /gene="hflD"
                     /locus_tag="RHW33_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV1646303.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(22367..23473)
                     /gene="mnmA"
                     /locus_tag="RHW33_002820"
     CDS             complement(22367..23473)
                     /gene="mnmA"
                     /locus_tag="RHW33_002820"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV1646304.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(23527..23988)
                     /gene="nudJ"
                     /locus_tag="RHW33_002821"
     CDS             complement(23527..23988)
                     /gene="nudJ"
                     /locus_tag="RHW33_002821"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV1646305.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(23998..24651)
                     /gene="rluE"
                     /locus_tag="RHW33_002822"
     CDS             complement(23998..24651)
                     /gene="rluE"
                     /locus_tag="RHW33_002822"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV1646306.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            24823..26073
                     /gene="icd"
                     /locus_tag="RHW33_002823"
     CDS             24823..26073
                     /gene="icd"
                     /locus_tag="RHW33_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV1646307.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         6330 a         6660 c         6806 g         6310 t
ORIGIN      
        1 tcatttagtt atcgatcgtt aagtaattgc ttgcgacgtc attcatctgc ataaggccac
       61 tattatgaaa aacgtaaaaa ccctcatcgc tgcggcgatt ttaagttcca tgtcatttgc
      121 cagctttgcg gctgtcgaag ttcagtcaac gccagaaggc caacaaaaag tcggtacaat
      181 cagtgctaac gcggggacaa atctgggatc gctggaagag cagctggcgc aaaaagcgga
      241 tgagatgggc gcaaaatctt tccgtattac ttctgtaacc ggtccgaata ccctccatgg
      301 aacagcagta atttataaat aagcattaac cctcattaat gcctgctact gctgattttt
      361 tccccgcgac atgccgtgtc gcggggattt ttttatccag gatttacaga gtttgtgggc
      421 tcacttgatg acggcggaca tccaccggca tcccggaacg gacatccatc acatgttgca
      481 tcacttcagc gtcagtttgt gctgcatttt taaatgattg cattgcgcta ttcagcgtaa
      541 ttggcagcag ctgcggatcg tcatcaatct tctctgacag cggctgatgt acttcaacca
      601 gacgcgcacc gtttggttcg gcagagactt ttatcggagt gttgatgata ttcactttgg
      661 tgcctggggt gacctggcta aagagtgttt tgatatcgtc atcccgcaga cgaatacagc
      721 cagaacttac ccgcatgcca atgccgaaat cggcgttcgt accatgaagc aaataaacgc
      781 cgccataggc cgccagacga atcgcatgat ggcccattgg gttatccggt ccagccggaa
      841 ccaccgcagg caactcaatt ccctgtgctt tataacgggc tcggatgttt gccgttggcg
      901 tccaggttgg gtttgcacgc ttgtcagaaa cggtggtcac cattgtcggt gtcagcgtgt
      961 caccacctaa ctgaccaata cctattggat acacggttac cgaattttta cccggcgggt
     1021 agtaatagag acgaagctcc gcaatgttga tcacaatgcc ttcgcgcggc gcgtctggaa
     1081 gtagggtttg caacgggatc gttaacacgc tgcccgcgcg cggtacgtaa ggatcaacgc
     1141 cggggttagc ctgtaataga gcgagaaagc cgacgttata ttttttggcg atggcttcca
     1201 gagaaccacc gtcattttcc accacatgaa atttgttttc gccaaccaga cgactgcccg
     1261 ctggcggcag cggccaggtg ttggcttttg ccggtagcgc cagcaccacg gcagcggcga
     1321 acgtaaaaaa cgttagccag cgagaaaaac gcgttttgat catcaccaaa aatccataat
     1381 aaatataagg ttattgtaat aaacagataa gcctgaatta tggatggtga cagtgtcgga
     1441 tagtgcaggg aagtgcaaag aatttgtaaa tgttgtagat ggggcgcaga aacgcccccg
     1501 atttacctta agcgatcgcg ttctcttcca gttcacgcat aaactggcga acccattcga
     1561 tacgcgtttt ccgctcgctc aaatcctgaa taaatttcag gcgcgtcgga ccatcaaggc
     1621 ggtaatgctg cggctgtttt tgcagcaaac caatcaacca ggccggatta acgtgattct
     1681 tctcggcaaa ttcgatcacc ccgcctttct cattaccttc cagcttcctg atccccagtt
     1741 tctgcgcttg ctggcgcaga cgggcaatat ccagcagggt acgcgccgga tccggcagca
     1801 ggccgaagcg atcgataagc tcgactttga tctcttccag ttcgttttcc gttttggcgc
     1861 tggcaatacg tttgtagaac gacagacgcg tgttcacgtc agggatgaaa tcatctggca
     1921 atagcgacgg catccgcagc tcgacttctg tttgctggct ggtgagatct tccagcgacg
     1981 gctcgcgtcc ggctttcagt gcatcgacgg cgttttccag caactccata tacagcgaga
     2041 aaccgatggt ttccattgag ccactttgtt cttcgccaag cagttcaccc gcgccgcgaa
     2101 tctccagatc gtgcgttgcc agcgcaaaac ctgccccgag atcttccagc gaggcaatcg
     2161 cttcaagacg tttttgtgca tcggtagtca tcgcttttgg atgcggtgtc agcaaccatg
     2221 catatgcctg atgatgcgaa cgtccgacgc gaccgcgtaa ctggtgcagc tgcgccagac
     2281 cgaagtgatc cgcgcgttca atgataatgg tgttggctgt cgggatgtcg atcccggttt
     2341 cgataatggt tgtacaaacc agcacgttga aacgttgatg atggaaatca ttcatcaccc
     2401 gttccagttc gcgctcgcgc atctgcccgt gaccgatggc gatccgcgct tctggcacca
     2461 gttctgccag ccgttcggcg gctttctgaa tgttttccac atcattgtag agataataaa
     2521 cctgtcctcc gcgcaaaatt tcacgcagga tcgcctcccg gaccaccagg ctgtcatact
     2581 cacggacaaa ggttttaact gccagacgac gggcgggcgg cgtggcgata atcgacaggt
     2641 cacgcattcc gctcattgcc atattcagcg tacgtgggat cggcgttgca gtaagcgtca
     2701 gaatatccac gttcgcgcgc atcgctttaa tgcgctcttt atgacgcacc ccgaagcggt
     2761 gttcttcatc gacaatcagc aggcctaaat ctttaaactt gacgtcactt tgcagcagtt
     2821 tgtgcgtacc gatcagaata tcgattttcc cttccgccac ttccgcaagg atttgcgtct
     2881 gctctttggc gctgcggaaa cgggagatca tttcgatgcg taccggccag ttggcgaaac
     2941 ggtcgcggaa gttgtcgtaa tgctgctgcg cgagaagggt ggtaggcacc agcaccgcca
     3001 cctgcttgtg gttatctacc gccaggaaag cggcgcgcat cgccacttct gttttaccaa
     3061 agccaacatc gccgcacacc agacgatcca ttgccagcgg ctgacacatg tcgctaagta
     3121 ccgcattaat ggcctgtgcc tgatccggcg tagtttcaaa cgggaagctg tcgcagaaca
     3181 actgatactg ctcacgatcg tgtttaaacg cgaagccctc tttggcggcg cgttgcgcgt
     3241 agatatccaa caattctgcc gccacatcac gcactttttc cgccgctttc tgccgcgcgc
     3301 gtgaccacgc atcgccgcca agtttatgca gcggggcgtt ttcttccgcg ccacctgcgt
     3361 aacggctaat cagatgcagt gacgacaccg gaacatacag tttggcgtcg ttggcatagg
     3421 tgagcatcaa atactcgcca gtaatgcctc cagcttcgag cgtggtcatt ccggcataac
     3481 gaccaacgcc gtgctccaga tggaccaccg gctgaccaat atgcagctcc gcgaggttgc
     3541 ggatcagtgt atcggggttg atggtgcggc gagagtcctg acgacggcgg gcaacgcgtt
     3601 caccgagcag atcgctttcg caaatcagcg ccaggttacg caccttatcg acaaaaccat
     3661 gttcggcagc gccaatcatc agataacgcc cacggtcgct ggcttcatca agacgcataa
     3721 tgcgttgcgg agcaatttta attcgtgcga gcagttcacc cagcgcttca cggcgacctt
     3781 cgctttctac cgagaacacc accggaccgt cgaaagactc gaggaactta cgcagcgcat
     3841 ccagcggcgc tttttgttgt gcctgaacgg ccaggtctgg cagcctctgg aaacctaaat
     3901 tggcattcgc tgcttttgtc ggtaaatgtt cagtttttag ctgcacgcgg ggccagtttt
     3961 tcagctctga gaagagctcg tccacccgca gccagagcga ttgtggtggc aacagtgggc
     4021 gcatcggatc gacgccgcga ttctcaaaac gcgccagcgt gtcagcctgg aaacgttcgg
     4081 cactgttttc cagatcgcca gtattcacca gcagcgtatt ggcaggaaaa taactgaaca
     4141 gtggcggcaa tggttcactg aagaatagcg gctgccagta ctcgatcccg gcaggtaatg
     4201 tgcctttact cacttgctga taaatatgtt ccgggtcgcg cttcacttcg aaggtatcgc
     4261 gccactggct gcggaacagt tcaatcgccg ctttatcggt cggaaattcg tgcgcgggca
     4321 gcagattgat cgcttctact tcctccagcg tgcgctggct gtcgacgtca aacacccgca
     4381 ggctgtcgat ttcatcatca aagaaatcaa gacgataagg cagctcactc cccatcggga
     4441 agagatccag caacgcgccg cgcgtggcgt attcgccgtg ctccatcacc tggtcaacat
     4501 ggcgataacc ggcgctgtcc agttgggttc gtaatgcatc tcgtgacagg cgctgacctt
     4561 ttttcatcac cagcgcatga ccgtggagaa aactgtgcgg gcaaacgcgc tgcataagcg
     4621 tattcaccgg aacaatcaat acgccacgct gcatcgtcgg tagctggtaa agggtggaaa
     4681 ggcgtgagga gataatgtcc tgatgaggcg aaaaactgtc gtagggaagg gtttcccagt
     4741 ccgccagatt catcaccatc tggtcggtga actggctgat ttcatcatgc aaacgcagag
     4801 cattttgcat atctggtgca atgagtacca ccggaccggc gtgacgttcg gcaatttccg
     4861 ctaccagcgt cgcgcaggca gcgccggtta actcgcccag cagacgctgc tcacccgctt
     4921 tgacgggcag cgtataacga tattgttcag gcataacggt tgtcagattc tcgttaggat
     4981 atgcctcaac atatgggggc atatctctga taagcaatgc tgttcattat ccgtaatcga
     5041 catggtttag caaatcgaat cgccccggta ggggcgatgg cttaacgaga gtaactggtg
     5101 gcgggcagag aaggtgggga gaacaacagg tcgctgaatg cgcgttgtga aaattttcgt
     5161 accagcaagc cagccagtgt acaaatcaac aaactgacaa agggataaac caacaggagc
     5221 gccagttcga cttgcggcga ccagcgcccg gcgttcattt gcggaatcag gcttaagctg
     5281 aatatctcca ccagaattct atgggtggtg tagatggcga tggtatttga accaatcaca
     5341 ttcagcaaac tggtggaacg catgccaaaa cgctgttcat actgataaaa caacttcatg
     5401 atcaccacaa tcgacaccag cgacaacagc agcgagatat taaacaacca ggcaccgacc
     5461 gccagaacag ccagcaaaga agccatcagc agatggcggc gcaacggcac cgctttaaca
     5521 caggtcatta acgttgcgcc aaaccatgcg ccaagactgt aataaggcaa attgcggatc
     5581 acgctgttca ttccccacca cggcgtggga acgaaattaa cagccacact caacagtaca
     5641 aacaaggtga atagtggcag cgccagacgg ctaaaaattt tacacaccac gaaatagaca
     5701 attaacgcat acagatacca caagctggtg ctggcggtga gcatcccgtg caggaactca
     5761 ccggtggaat cggcataagc agcattggag gcattgctta aatcgcgctc aggtgccagc
     5821 cactgattca gcgcacttaa cgccagccac tggacaatgc cccaaagcgc cagcacccag
     5881 aagatgctcc agatgcgttt atcgagacaa tttccccacg gcacgctgtc gatatagcgg
     5941 cgaatcaaat agccggaaat aaagaaaaaa accggcatac gaaagggcgc aaggtaaaga
     6001 ttgaaataga tccagcattt gctcaggact tctgataacg gatgctgaaa agtggtcata
     6061 tgcggataaa aggtaatgac cgagtgataa attactacca gacaaatgca taaccctttg
     6121 atctggttga tccatagctc tttttgtttc atcaggacaa cacgctcttt tttaatttac
     6181 atgcggttga tcctgcgggt tatgacccga ggtgtaatgg gtatctgtct gtattggatg
     6241 atttttcaga ttaagatcag gcggcaagat tgatgataaa acatggcaat ttagccgatt
     6301 gatttacggg ggcttttcag attagccctg acgatcactt acagttcaga cgtttaccca
     6361 tcttgctttc gcttatatac tcgtgtcttt gctacagcaa ccagacggat ttcatgtacc
     6421 aacctgtcgc tctatttatt ggcctgcgtt acatgcgtgg gcgggcagcg gatcgcttcg
     6481 gtcgtttcgt ctcctggctt tctaccatcg gcattaccct cggggtgatg gcgctggtca
     6541 cagtattgtc agtgatgaac ggctttgagc gcgagctgca aaacaacatc cttggcctga
     6601 tgccacaggc aattctctct tctgagcatg gctctcttaa cccgcagcaa ctcccggaaa
     6661 cggcagtcaa actggacggc gttaatcgcg tcgcacctat tactaccggt gatgtggtac
     6721 tgcaaagcgc gcgcagcgtg gcggtcgggg tgatgctcgg tatcgacccg gcgcaaaaag
     6781 atccactaac gccgtatctg gtcaatgtga aacaaactga cctcgagccg gggaaatata
     6841 atgtcatcct cggcgaacaa cttgcctcac agctaggcgt taatcgcggt gatcaaatcc
     6901 gcgtgatggt accatctgcc agccagttca cgccgatggg gcgtattcca agtcagcgcc
     6961 tgttcaatgt gattggtact tttgccgcta acagtgaagt cgatggctat gaaatgctgg
     7021 tgaatattga ggatgcctcg cgtctgatgc gttatccggc aggcaatatt accggctggc
     7081 gtttgtggct ggatgagccg ctgaaagttg actctttaag tcagcaaaaa ctgcctgaag
     7141 gcagcaaatg gcaggactgg cgtgaccgta aaggcgagct gttccaggcc gtacgcatgg
     7201 aaaaaaatat gatgggctta ctgctgagcc tgattgtcgc cgttgcggcg tttaacatta
     7261 ttacctcgct ggggctgatg gtgatggaga agcagggcga agtagcgatc ctgcaaacgc
     7321 aaggcttaac tccgcgacaa atcatgatgg tctttatggt gcaaggggcc agcgccggga
     7381 ttatcggtgc gatcctcgga gcggcgcttg gcgcactgct tgccagccag ttaaataatc
     7441 tgatgccgat aatcggcgtc ctgcttgatg gcgcggcgct gccggtggct atcgaacctt
     7501 tacaggtcat tgttattgcg ctggtggcga tggctatcgc gctgctgtct acgctttacc
     7561 cttcatggcg cgctgccgcc acacaacccg ctgaggcttt acgttatgaa taagatcctg
     7621 ttgcaatgcg acaacctgtg caaacgctat caggaaggca gtgtgcaaac cgatgtactg
     7681 cacaatgtca gtttcagcgt gggcgaaggt gaaatgatgg cgatcgtcgg tagctctggt
     7741 tccggtaaaa gtaccttgct gcacctgctg ggcgggctgg atacgccaac ctccggcgat
     7801 gtgatcttta acggtcaacc gatgagcaaa ctgtcttcgg cggcgaaagc agaactgcgc
     7861 aaccagaagc tgggctttat ttatcagttt caccacctgc tgccggattt tactgccctg
     7921 gaaaacgtgg ctatgccgct gctgattggc aagaaaaagc ccgctgaaat caacagccgt
     7981 gcacttgata tgttaaaagc ggtggggctg gagcatcgtg cgaatcaccg cccatctgaa
     8041 ctttctggcg gcgaacgcca gcgtgtggcg attgcccgtg cgctggtcaa taacccgcgg
     8101 ctggtactgg cggatgaacc taccggtaac ctcgatgcgc gtaacgccga cagcatcttc
     8161 cagttgcttg gggaattgaa tcgcttgcag ggcaccgcct tcctggtggt tactcacgac
     8221 ctgcaactgg cgaaacgtat gagccgccaa ctggagatgc gtgatggtcg tctgacggcg
     8281 gaactgagcc tgatgggggc ggagtaatgg cgatgccttt atcgttattg attggcctgc
     8341 gttttagccg cggacggcga cgcggcggca tggtgtcgct gatctccgtc atttctacca
     8401 ttggcattgc ccttggcgtg gcggtattga tcgtcggctt aagcgcgatg aacggctttg
     8461 aacgcgaact gaataaccgc attctggcgg tggtgccgca tggcgaaatc gaggcggtgg
     8521 atcagccgtg gactaactgg caggaagcac tggatcacgt gcaaaaagtg ccaggtattg
     8581 ccgccgctgc gccgtatatc aatttcaccg gactggtaga aagtggtgcc aatctgcgcg
     8641 caatccaggt gaagggcgtt aacccacaac aggaacagca tctgagcgca ttaccctcgt
     8701 ttgttcaggg ggatgcctgg cgcaatttta aagcgggcga acagcaaatt atcatcggca
     8761 aaggcgtggc ggatgcgctg aaagtgaagc agggcgattg ggtgtcgatc atgatcccca
     8821 actcgaatcc cgagcataaa ctgatgcagc caaaacgtgt gcgtttgcac attgccggta
     8881 ttttgcagtt gagtggtcaa ctcgatcaca gttttgccat gatcccgctg gcggatgccc
     8941 aacaatatct tgatatgggt tccagcgtgt caggtattgc ccttaaaatg acggatgttt
     9001 tcaacgccaa taagctggta cgcgatgcgg gtgaagtgac caacagctat gtttatatta
     9061 aaagttggat tggtacttac ggctatatgt atcgcgatat ccagatgatc cgcgccatta
     9121 tgtatctggc gatggtactg gtgattggcg tggcctgttt caacatcgtc tccaccttag
     9181 tgatggcggt gaaagacaag agtggcgata tcgcagtatt aagaacgctg ggggcgaaag
     9241 atggtttaat tcgcgccatc tttgtctggt atggattgct ggcagggctg ttcggcagcc
     9301 tgtgtggtgt gattatcggc gtagttgttt cactgcaact taccccgatt attgagcgga
     9361 ttgaaaagct gatcggtcat cagttcctct ccagcgatat ctattttatt gacttcctgc
     9421 catcggaatt gcactggctg gacgtcttct acgtactggt cacagcattg ttgctgagtc
     9481 ttttggcaag ttggtatccg gcgcggcgcg ccagtaatat tgaccctgcg cgagtcctta
     9541 gcggccagta aaggcagtac attaaaacaa ggagtggcaa tgtattacgg gtttgatatt
     9601 ggtggaacaa aaattgcgct tggcgtgttt gatagcggtc ggcagttgca gtgggaaaag
     9661 cgggtgccga caccgcgtga cagctatgac gcatttttag atgcagtgtg tgagctggta
     9721 gctgaagctg accaacgttt tggctgtaaa ggttctgtcg gcatcggtat tccgggaatg
     9781 ccggaaacag aagatggtac gctgtatgcc gctaatgtcc ccgctgccag cggtaaaccg
     9841 ctgcgtgccg acctgagcgc acgtcttgat cgcgatgtac gccttgataa cgatgccaac
     9901 tgttttgccc tttcagaagc ctgggatgat gaatttactc aatatccact ggtgatgggg
     9961 ttgattctcg gcactggcgt tggcggcggg ctgattttca acggcaaacc aattaccggg
    10021 aaaagctaca ttaccggcga gtttggccat atgcgtctgc cggttgatgc gttaaccatg
    10081 atggggttgg atttcccgtt acgccgctgc ggctgtggtc agcatggctg cattgaaaat
    10141 tatctgtctg gtcgcggttt tgcgtggctg tatcaacact attatcatca accgttgcag
    10201 gctcccgaaa ttattgcgct ttatgatcaa ggcgacgagc aggcaagggc gcacgttgag
    10261 cgttatctgg atttattagc ggtttgtctg ggaaatatcc tgaccatcgt tgaccctgac
    10321 ctggtcgtca ttggtggtgg cttatcgaat ttcccggcaa tcacaacgca actggcggac
    10381 aggctgcctc gtcatctctt acctgtagct cgtgttccgc gcattgaacg cgcgcgccac
    10441 ggtgatgcgg gaggaatgcg tggtgcggcc ttcctacatc taaccgatta aacaacagag
    10501 gttgctatgc tgtcgcgtcg gggtcatcgg ttaagtcgtt ttcgtaaaaa taaacgccgc
    10561 ctgcgcgagc gtttgcgtca gcgtattttt ttcagagata aagtggtgcc ggaagcaatg
    10621 gaaaaaccaa gagtactcgt actgacaggg gcaggaattt ctgcggaatc aggtattcgt
    10681 acctttcgcg ccgcagatgg cctgtgggaa gaacatcggg ttgaagatgt ggcaacgccg
    10741 gaaggtttcg atcgcgatcc tgaactggtg caagcgtttt ataacgcccg tcgtcgacag
    10801 ctgcagcagc cagaaattca gcctaacgcc gcgcatcttg cgctggctaa actgcaagat
    10861 gctctcggcg atcgcttttt gctggtgacg cagaatatag acaacctgca tgaacgcgca
    10921 ggtaatacca atgtgattca tatgcatggg gaactgctga aagttcgttg ttcacaaagt
    10981 ggtcagcttc tcgactggac cggagacgtt accccagaag ataaatgcca ttgctgccag
    11041 ttcccggcac ccttgcgccc gcacgtagtg tggtttggcg aaatgccact cggcatggat
    11101 gaaatttata tggcgttgtc gatggccgat attttcattg ccattggtac ttcggggcat
    11161 gtttatccgg cggctgggtt tgttcacgaa gcgaaactgc atggcgcgca caccgtggaa
    11221 ctgaatcttg aaccgagtca ggttggtaat gaatttgccg agaaatatta cggcccggca
    11281 agccaggtgg tgccagaatt tgttgaaaag ttgctgaagg gattataaag caggaagcat
    11341 tgcctgatgc gctacgctta tcaggcctac aagcggacca caataccgac gaaagagcgg
    11401 gataaaactt tactgtcctg actcattagc cacatccttg ctaactaaaa aacgggcggt
    11461 aataccaccg cccgcttgct gaattaacgt cctgctttca gcttctgata atactcttca
    11521 taaatgctgc tggctgcgcc aacgtcattt tgccattcac catttttaat ggtttcagca
    11581 tccgggtaga gcgtcttatc gttcgccact tctggactta acagcttacg cgccgcaagg
    11641 tttggcgttg gataaccgat agtttcagca acctgttttg ccacatccgg gcgcagcagg
    11701 aagttgatca atttcagcgc gccttctttg tttttggcat ttgccgggat cgccaggctg
    11761 tccatccaga atatgccgcc ttctttcggc cacaccacgt caataggcgt acccgcctgg
    11821 cgtgcaacga aagcagaacc gttccagatc atgccgaggt taacttcgcc ttccatgtac
    11881 gggttagccg ggttatcgga gttaaacgct gcgacgtttg gcatcagttt tttcagctcg
    11941 ttatatgcag cttcaatctc tttcggatcg gtggtgttac cggagtagcc cagcttacgt
    12001 agcgccatct ggaacacttc acgggcatcg tcggtcagca gcaggctgcc tttgtactca
    12061 ggcttccaca gatcggccca gctggtaacg gatttcggat ccaccgcatc accgttaacg
    12121 ccaatcgccg tcgcccccca gatatacgga atggagtagt cgttattcgg gtcaaacggc
    12181 ttgttgagca tgtctggatc gaggttgctg aaattgctta acttcgactt gtcgatcttc
    12241 tggatcatcc cttctttacg cattttatcg acgtaatagg ttgaaggaac caccaggtcg
    12301 taggcaccgt ctttgtacgt tttcagcttg gcgtacatgg tttcgttcga ctcgtaagtc
    12361 gaatagataa ccttaatacc tgtttctttg gtgaactgtt caagcagtcc tggcggcacg
    12421 tactcggtcc agttgtagaa atacagcgta ttgttgtcat cggcgtgagc ggcgctcatg
    12481 cccagtgcca gaacacccgc cgcgagcagg tggcgtgacc attttttcat ttaacgtccc
    12541 ctgtgttacc tttcgtttta tcacgagcaa taagctggct ggcaattacc atcaccagcg
    12601 acagcaccag taatatggtt gccagcgcgt taacttccgg cgatacgccg actttgacca
    12661 tcgaataaat ttttaacggc agaatttcat agcttggtcc ggtgacgaac gaagaaacta
    12721 ctacatcgtc catcgacagg gtaaagctta acacccagcc cgccgccacc gcaggcattg
    12781 ccagtggcag aatgattttt cgcagaatgg taaattcact ggcaccgaga tctttcgccg
    12841 cttccagcat tcgcacgtca aaacctttca ggcgcgaata caccgtcacc accacaaatg
    12901 gcaggcagaa ggtgatatgc gagaacagca gcgaccagaa gccaagctga atacccagca
    12961 gcataaacag caccagcaga gaaatcgcca tcacaatatc tggcgacatc atcaccacaa
    13021 acagcattcc gctaacgaac ggcttaccgc gaaagcgata acggtacagc gcaactgcag
    13081 tcagtgaacc gataagcgta gcaaacgtcg ctgaaaacac cgccatcgtc aatgaatgct
    13141 gagctgcctg caacagactg tcgttgttca tcagcaggct ataccattta gtggtaaaac
    13201 cctgccagtt gatgccaaag cgcgagctgt tgaaagagtt cacaatcaaa ataatgattg
    13261 gaatatacag gtacgcgtag atagcggtca taaaaccgcc gcgaagcagt cgaccgatca
    13321 ttcgagttcc acctttttat tcagcagacg agaagcgcgc cagtaaacca gcaacatcag
    13381 gcccattacg atagtcagcg taatgctggt ggctgcacca aacggccagt cacgaatatt
    13441 gaggaactgt accttgatga cgttaccgat cagcaggttt ttcgcaccgc ccatcaggtc
    13501 ggacacatag aacaggccca tcgctggcag catcaccagc agacatccgg caataatccc
    13561 cggcatcgtc agtggaataa tgatgcggat aaaagtctgt aacttgctgg caccgagatc
    13621 gcgcgccgcc tcaagcaacg gcttatccag tttttcgata ctggagtaca gcggcatcac
    13681 cataaacggt agcagaatgt aaaccagacc gataatcacc gcactgggcg tgaacatgat
    13741 acgaattggt gtgtcgataa cgcccagcca gagcaaaaac tcgttgagat agcctttggt
    13801 gctgaggaaa attttcagcc cgtagatgcg aatcagtgag ttggtccaga agggaacaat
    13861 cagcagaaac agcagcagcg gacgcacctt gtgtggcagc ttcgccagaa accaggcaaa
    13921 cggatagccc agcaccaggc aggcgagggt ggcgatcagc gccatattca gcgagtgcaa
    13981 tagcacttca aaatagagcg gatcgagcag acgcgtgtag ttatccagcg taaagaccat
    14041 tttgacaaaa ctggcgtcgt cgcgggtcaa aaagctggtg ccaatgatca tcaggttggg
    14101 cagaaagaca aacaacacaa gccaaccgac aatagtgaca atcactacat tctggaactt
    14161 acttgtgttc ttcatcagcc agtacgacct cccagctttc tacccaatta atggccattt
    14221 tttggtcgag agagtggtca aagtcaggat cgtcttcatt gaagaattcg ctgaccatca
    14281 ccatcttgcc attttccagt tcaacaaccg actccagcgt catgcctttg tagttacgct
    14341 cgcgaacgta accaatcagc ccttcagcgt ggttgtcgtc gttaatctct tcaacacgta
    14401 agtcttccgg gcgcagcaga acatgcagtt tttgccccgg ttcaacggcg aagttaacgt
    14461 agatattaca ttctcggcct tcaacgttgg cgcgtacgcg ctgctcgtcg agacgttcga
    14521 tgacagtggc gttaaacata ttgatttcgc caatgaaccc ggcaacaaac aggtttttcg
    14581 gctcttcgta gatttcacgc ggcgtgccgt cttgctcaat gcgaccatcg cgcatcacca
    14641 caatcctgtc tgacatggtg agtgcttctt cctgatcgtg agtcacgaag acgaatgtaa
    14701 tgccaagctt acgctgtaac gctttcagct cgttctgcat ttgcttacgc agtttgtaat
    14761 ccagcgctga gagcgactca tccagcaaca acagacgagg cttgttaacc accgcgcgag
    14821 caatggcgac gcgttgctgt tgaccaccag agagctgatg cggtttgcgt tgagcgaagg
    14881 tttccagctg caccatccgc agggcttcca tcacgcgggg cgtaatttca gcagcggggg
    14941 ttttttgcat gcgcaaccca aaggccacat tttcgaacac ggtcatgtgg gggaaaagtg
    15001 cgtagctttg gaaaacagtg ttcacatagc ggttttccgc cggaacgtgg gtgatgtcct
    15061 cgttatccaa catgatgcgt ccggaatcaa cagtttccag acctgcaatc aggcgaagaa
    15121 cggttgtttt accgcagcca gaagggccaa gcagcgtgag gaactcgcca ttgttgatag
    15181 tcagatccag ctggggaatg acctctttac catcaaagca tttgcgaatt cccgccaatt
    15241 gcaccagcgg tgaaagcgaa ctcggttgtt tattcaattt tttactctgt cccatgtaaa
    15301 cgcaacggat ggcttaccga tgcggggttt gtggttaacc accttggtga ctcttaatga
    15361 gggcggtaat tctacggcaa accgcttgaa tcgccaatct ttgttgtgaa ttactggctt
    15421 agctttatat tcattaaggt aatgctgata aatattcccg cttgcagggg taaaagtgac
    15481 ctgacgcaat atttgtcttt tcttgcttct taataatgtt gtcacaaaaa gtgagggtga
    15541 ctacatggat aaactacttg agcgattttt gaactacgtg tctctggata cccaatcaaa
    15601 agcaggggtg agacaggttc ccagcacgga aggccaatgg aagttattgc atctgctgaa
    15661 agagcagctc gaagagatgg ggcttatcaa tgtgacctta agtgagaagg gcactttgat
    15721 ggcgacgtta ccggctaacg tccctggcga tatcccggcg attggcttta tttctcatgt
    15781 ggatacctca ccggattgca gcggcaaaaa tgtgaatccg caaattgttg aaaactatcg
    15841 cggtggcgat attgcgctgg gtatcggcga tgaagtttta tcaccggtta tgttcccggt
    15901 gctgcatcag ctactgggtc agacgctgat taccaccgat ggtaaaacct tgttaggtgc
    15961 cgatgacaaa gcaggtattg cagaaatcat gaccgcgctg gcggtattgc aacagaaaaa
    16021 cattccgcat ggtgatattc gcgtcgcctt taccccggat gaagaagtgg gcaaaggggc
    16081 gaaacatttt gatgttgatg ccttcgatgc ccgctgggct tacactgttg atggtggtgg
    16141 cgtaggcgaa ctggagtttg aaaacttcaa cgccgcatcg gtcaatatca aaattgtcgg
    16201 taacaatgtt catccgggca cggcgaaagg agtgatggta aatgcgctgt cgctggcggc
    16261 acgtattcat gcggaagttc cggcggatga aagcccggaa atgacagaag gctatgaagg
    16321 tttctatcac ttggcgagca tgaaaggcac cgttgaacgg gccgatatgc actacatcat
    16381 ccgtgatttc gaccgtaaac agtttgaagc gcgtaaacgt aaaatgatgg agatcgccaa
    16441 aaaagtgggc aaagggttac atcctgattg ctacattgaa ttggtgattg aagacagtta
    16501 ctacaatatg cgcgagaaag tggttgagca tccgcatatt ctcgatatcg cccagcaggc
    16561 gatgcgtgac tgcgatattg aaccggaact gaaaccgatc cgcggtggta ccgacggcgc
    16621 gcagttgtcg tttatgggat taccgtgccc gaacctgttc actggcggtt acaactatca
    16681 tggtaagcat gagtttgtga ctctggaagg tatggaaaaa gcggtgcagg tgatcgtccg
    16741 tattgccgag ttaacggcgc aacggaagta agcgaaaagg gatgcggcat gtgatgccgc
    16801 atccggctta aatccaaact tacccttcga agaaccaata cccgctattg accagcgccg
    16861 cgagcatcgc gaggaatgac ggatcttcca gcgcatcgcc aaaattctcc gcagtcagcg
    16921 caatgttgct ggcgagtgca tccagtgccg gacggtgcgg ggaatcgatc ttctcaccat
    16981 tggcatacac gtcgtcgcca atgcgcaata cgcgcagacc acccaggcgc accagcacat
    17041 caccttgttt cagcgcatcg tagatttcat ccggctgata aggcggctct ggcggcgcga
    17101 tatccagttc atgacgtgac tgggatataa actccccaaa ccattgctta aagtgttccg
    17161 gctggttgat caattcgagc atcatctcac gcagtttatc catctcttgc ggcagaacat
    17221 ccgcaggatg agcgcgaggt ggaacatcag gatcgctgta gtagttgccg cccagttcac
    17281 gttgcagcac ataatcggca aatccactaa tcagttcccg cgtatttggc gcgcgaaaac
    17341 ccacggaata gttcatcgca ttttccagcg cgtagccttc atgcgggaat cctggcggaa
    17401 tataaagaat atcaccaggc tccagctctt catcgatgat ggcttcgaac ggatcgacct
    17461 gtaacagatc cggatgtggg cagtgctgtt tcatttgcag cttctcgccc actcgccagc
    17521 gacgacgtcc ggtaccctga atgataaaca cgtcgtactg atcgagatgc gggccgacgc
    17581 cgccgccagg tacagaaaaa gagatcatca gatcatcaat acgccagtcc ggtagttcac
    17641 ggaacggtcg catcagcgcg gcggtcggct catgccagtg gttcactgct tgcactaaca
    17701 atgaccagtt agtttcaccg agatgatcgt agctttcgaa cggaccgtgg ctgacctgcc
    17761 attttccatc ctggtgactg accagtcgac tgtcgacttc gctttccatc gccagacccg
    17821 ccaactcgtc tggagagatc gggtcaataa aattattaaa gccgcgtttt aacaccaccg
    17881 ggcgtttctg ccagtgacgt tcaagaaaat cgggccagtt aagagtgagt tggtattcca
    17941 tgttaagcat ccacaggctg gtatctgcaa ccgattataa cggatgctta acgtaatgcg
    18001 tgaagtatgg acatatttat tcatctttcg gcgtagaatg ctggcgacca aaaatcacct
    18061 ccatccgcgc accgccaagc atgctctctc cggcgacgat tttaccctca tattgctcgg
    18121 tgatttcgcg ggctaccgcc agccctacac cttgcccagg gcgtaaagta tcaacccgtt
    18181 gaccacggtc gaaaatgacc tctcgcttgc ttaatggaat accgggtcca tcatcctcga
    18241 ccacaatata gagatgctcg tcggtttgcc ttgcagaaat ttcgacaaac tcgaggcaat
    18301 atttacaggc attatccagc acattgccca tcacctcgac aaaatcgttc tgctcaccga
    18361 caaagctgat ctctggcgaa atatcgagag agatattgac ccctttgcgt tgatacactt
    18421 tgttcagcgc cgaggtgaga ttgtccagca gtggggcgac cggatgtagc tcgcggctaa
    18481 gcaatgtccc gccgcgcata ctggcacgat gcaggtagta gccaatttgc tgtgaaatgc
    18541 ggctgatttg ctccagcatt accggttcag catcactgac gctcatcttt tcactacgca
    18601 gagaacgcag cgtactttgc agcaccgcca gtggcgtttt cagactatgg gtcaggtcgg
    18661 tgagcgttgt acgatatttg tcgtaacgtt cgcgttcact ttttaacaat cggttcaggt
    18721 ttcgtaccag actggtcagt tctcgcgttg tggctggatt gagcaattcg cggttatgtt
    18781 cttccagttc gcggacttct tttgccaggg cttcgatggg gcgtaaactc caccaggcgg
    18841 cgacccacag cagcgggatc actaacagca gattggctga gagcacatag ataaaccagc
    18901 tccagaccat ataggaactt tttagctcca ccggaatggt atccaccacc acaatggtta
    18961 actttggcat ccgcgatgtt gccgggtaga cgtttaccgc caccgagtgg gtcatctccg
    19021 cgtcgtcatc atcttcccgc acttcctgca actgttgctg tatcgaatga tctccactca
    19081 gcaagaggct ggtatcgtta acatccgctt caatttcatg aaaaccattc gatttcagcc
    19141 agtcaggctg gatcatcttc atcagccagg gcacgtcacg ttgcgcccat aaaagctgcc
    19201 cgttctcatc ataaattagc gtcatggtgg ggctttgctt gtcgatattt tcgggtaact
    19261 cgacatgcaa cttattgttt tcccacttcg caagggtata gaacagattg ctctcgccac
    19321 gtaacagccg aaacgtagtt ttatcgaaac tgacgctata accgatcagc gcgaccattc
    19381 cgtaggcaag cgaaagcacc agtactaccg ctgccgttgc taacagaaaa cgtacccgca
    19441 gcgagagcgg gaaaaaaaga cgcagtaatt ttttcatcag cgcaattcga acaaatagcc
    19501 ctggccgcga acggtagtaa tcacttcttg cggatattgt gcctgaattt ttttgcgcag
    19561 acgtcccatc agtacatcaa tggtatggct ttcccgcagc tccgcatccg gatagagttg
    19621 tagcattaac gaatctttgc tgaccacttt gccattattg cgtatcaacg tttccataat
    19681 ggtgtattcg aacgcggtca gtttgatcac ttcgtcatta atagataatt cacggcgaga
    19741 gagatcaacc tgaaacgggg ggagcgaaat gacctgtgaa gccaggccgc tattacgccg
    19801 cattaatgcc tgcattcgcg ccatcacctc ttcaatatga aacggtttag tcacgtaatc
    19861 atcagcaccg gcacttaata cttcgacttt gtcctgccag ctttcacggg cggttaatac
    19921 cagaatcggc agtgaaacat cattgctacg ccagcggcga atcagtgaca gaccgtcctc
    19981 gtctggcaat ccgagatcga caatcgcaat atccggtaaa tgttcattga gataataatc
    20041 ggcttctttg gcatcttctg catcatcgac ctgatgacca gcatcctgaa tctgaacttt
    20101 aaggtggtga cgtaacaacg cattgtcttc aacaaccagt acgcgcattt ttatttctcc
    20161 ctgtcttaat tattaaaata gtgtaacgcg attatgtggt tatgggggta aacattaaat
    20221 aaaccagcgg ggaggggagg taaagtgaaa aaataaaaag cggataatct taataagcag
    20281 gccggacagc atcgccatcc ggcactgata cgagtttatt tcagctcatc aaccatggtg
    20341 atggcgcgac caatgtagtt tgccggcgtc atcgctttca ggcgggcttt ctcttcttct
    20401 ggcaacgcca gaccgtcgat aaactgcttc atgccttcgg cgtcaacgcg cttaccacga
    20461 gtcagctctt tcaatttctc gtacggtttt tcgatgccat agcgacgcat aactgtctgg
    20521 attggttctg ccagcacttc ccagttgtga tccagctcat ccagcagatg gtcacggttc
    20581 acttccagtt tgctcacgcc tttcagggtg gattgatacg caatcagcgc ataaccgata
    20641 cccacgccga ggttacgcag cacggtggag tcggtcaggt cacgctgcca gcgggaaacc
    20701 ggcagtttgc ttgccagatg ctgtaatacc gcgttggaaa ggcccagatt cccttcggag
    20761 ttctcgaagt cgatcgggtt aactttatgc ggcatggtgg aagaaccaat ctcaccggcg
    20821 atggttttct gtttgaagtg gttaagggcg atataacccc agacgtcacg gtcaaagtcg
    20881 atcagaatgg tgttgaagcg cgcaacgcaa tcaaacagtt cggcaatgta gtcgtgcggt
    20941 tcaatctggg tagtgtacgg gttccattga atacccagcg aggtgacgaa ctcttcgctg
    21001 aactgatgcc agtcaacttc cgggtaagcg gcgatgtggg cgttatagtt accgaccgcg
    21061 ccgttgattt tgccgaggat ctccacctgg ttaagctggc ggtactggcg ctccatacgg
    21121 taggcgacgt ttgccatctc tttaccgatg gttgacggcg tggctggctg accgtgggta
    21181 cgagacagca gcgggatatc gcggtactga acggcgagat ctttaatgcc atcaattagt
    21241 tgacgccagt acggcaggat cacttcatca cgcgcggttt tcagcattaa tgcgtgggag
    21301 aggttattga tatcttccga agtacaggca aagtggatga attcagaaac cgcgtgcagt
    21361 tccgggatct ccgccacttt ttctttcagg aaatactcaa ccgctttaac gtcgtggtta
    21421 gtagtacgct cgatggtttt gatgcgtgcg gcatcttctt cgctgaaatt ggcgacaatt
    21481 gcatcaaggt aaccgattgc gtcggcagca aaagcaggaa cttccttgat cgctgcgtgc
    21541 gcggccagtt tttgcagcca acgtacttca acttgtacac ggaatttcag caaaccatat
    21601 tcgctgaaaa tcccgcgcag cgcgctgact ttatcgccgt agcgtccatc gacaggggaa
    21661 acggcggtca gtgaggataa ttccatagat cacaactccg gggttaaatg agcaagaatt
    21721 tgttttgcct gagtggtcag gcgattacga gaaaacatca gttgcagacg tccgccgccg
    21781 acctggtgcc agagcacggc ggcgcgaatg cctgccagca gggttgcgcg aactttcgcc
    21841 tgcacttgtg ggctttgcag tacagcaggg gagccggtga cctgaatgcg cgggccaagc
    21901 gggctaatca catcaacata gatagcagcc atcgcgctca tcagcgtttc ggactgtaaa
    21961 tcgaagtgtt cgagctggcg ttgcaggccg ttgatccggt tgcccagagt gtcgagcgca
    22021 cctttcgctg aggagagttt gcgctcaagc accatcaggc tgagtgtgta gcgggttaat
    22081 tcggcgttta agccctggcg actgctggca ttgagcacgc cgagcagggt ttccagcccg
    22141 acgcgcaggt tggcttcgct accgccaaaa accgccagcg tcgagctggg gttcatatca
    22201 ataatactgt tgagtgagac gtgtagcgca tcggcatcac aatgcccctg gtgagcgagt
    22261 tgttgcacca ggcgtgccga ctgacaaata ccggccaggg cgagggtgat gtcatagtaa
    22321 ttctttgcca cgttcactgc ttccttgttt taagtaaaga taataatcag accggcagcg
    22381 gcagacgctg ctcaataata ccgccaccga ggcacacttc accgttatag aagacggcag
    22441 actggcccgg cgtcacggcg gcaaccggct catcgaaaat cacttcaatg cgatcatcgt
    22501 ccagcgcctt gacggtgcag gggatatcgg tctggcgata gcgggttttt accgtgcaac
    22561 gcatagtgcc ggtaaatggt tcgcgatcga cccagtgcag ctgctgggca atcagaccga
    22621 cagacatcag acgcgggtgt tcatggccct gcgcaacaat cagaatgttg ttttcgacgt
    22681 ctttatcgac cacataccac ggttcttcgg tgccttcttt ggtgccaccg atacccaggc
    22741 ctttgcgctg accgagggtg tgatacatca gcccctgatg ctcaccaatt tcatcgccat
    22801 cgacggtaat gattttgccc ggttgcgccg ggagataacg gcccaggaac tcgcggaatt
    22861 tacgttcgcc aatgaagcaa atgccggtag agtctttttt cttcgcggtg accagaccaa
    22921 gatcttcagc aatcttacgc acctgcggtt tttccagttc gccgaccggg aacaggcttt
    22981 gcgcaatctg ctcatggctg agcgtataaa ggaagtagct ctggtcttta ttgctgtcca
    23041 gaccacgcag caggcggctc ttgccatcga catcggcccg acgaacgtaa tgaccggtag
    23101 cgatataatc ggcacctaaa tcttcggcgg caaattcgag gaaggcttta aatttgatct
    23161 ctttgttgca cagaatatcc ggattcggcg tgcgaccggc tttatattcg gcaaggaaca
    23221 gttcgaagac gttgtcccag tactcggcag caaagttaac ggtgtgcagt tcaatgccga
    23281 gcttgtcgca gacagcctgg gcatcagcca gatccgctgc cgctgtgcaa tattcctcac
    23341 cgtcgtcttc ttcccagttc ttcataaaca ggccttcgac ctgatatccc tgttgttgca
    23401 acagccaggc agaaacggag gaatcgacac cgccggacat gccgacgatt actttttttg
    23461 cggtttcaga cattggatca ctcacgacat tgaacttcaa ggcggcgtat tctatcacgc
    23521 agccttttag atgacaccct ttgtaaaagg ccagttaaaa tcgccaatca tctccagcgg
    23581 atagcgttgc ccgctttgat aacaacgaat actttccgcc accagtggcg aacgaagatt
    23641 tgacgcctgt aaaatttctt cggcgctaac ccaacggcag cagtcgatat cgctgtcatg
    23701 aggctgggtc gggcatattt gctcaagctc aatggcaaaa aggaaacgca aaaacggcgt
    23761 tttatctggc gcaatccact gatgcatacg aataaagtgt tgcggctgcg cgctgatgcc
    23821 ggtttcttcc cacagctcac gggcggcggc ttccactaag gtttcatccg cttccagatg
    23881 ccccgccggt tggttccata acgctttacc attaatcgtc tcttcaacga ctaaaaattt
    23941 gccttctgcg tgcaccacgc aagcaacggt aacgtgcggt ttaaacattt tgcatcctta
    24001 atctgtcact tctcgccatt cgccattagc aagattatcc aaagagtaat cacccatcgc
    24061 atagcgaatc agtcgcagcg tggggaaacc aacatgggcg gtcatgcggc gcacctggcg
    24121 attacgtcct tcatataagg tgatcttcag ccagctggtg ggaatacttt tgcgttcacg
    24181 aattggtgga ttccgcggcc ataaccacgc aggttcgtca accagctccg cgccggcggg
    24241 cagggtaggg ccatcattta aggttacgcc attgcgcaag gcttcaagtg cgtcttgtgt
    24301 gggaatacct tccacctgca cataatagat ttttccggtg cgtttacccg gctgggttaa
    24361 acgcgcctgc aacgcgccgt tattggtcag caccagtaac ccttcgctat cgcggtcaag
    24421 acgacctgct gcataaacgc cctgaaccgg gatgaattct tttaatgttt tgcgtccggc
    24481 ttcatcggtg aactgcggaa gaacatcgta gggtttattg aacaggatca cacgcgtggg
    24541 ctggttttca ggtttacgcc tggtagaacg ttgtgagctg aatcgcttaa cctggtgatt
    24601 tctaaaagaa gttttttgca tggtattttc agagattatg aattgccgca ttatagccta
    24661 ataacgcgca tctttcatga cggcaaacaa tagggtagta ttgacaagcc aattacaaat
    24721 cattaacaaa aaattgctct aaagcatccg tatcgcagga cgcaaacgca tatgcaacgt
    24781 ggtggcagac gagcaaacca gtagcgctcg aaggagaggt gaatggaaag taaagtagtt
    24841 gttccggcac aaggcaagaa gatcaccctg caaaacggca aactcaacgt tcctgaaaat
    24901 ccgattatcc cttacattga aggtgatgga atcggtgtag atgtaacccc agccatgctg
    24961 aaagtggtcg acgctgcagt cgagaaagcc tataaaggcg agcgtaaaat ctcctggatg
    25021 gaaatttaca ccggtgaaaa atccacacag gtttatggtc aggatgtctg gctgcctgct
    25081 gaaacccttg atctgattcg tgaatatcgc gttgccatta aaggtccgct gaccactcct
    25141 gttggtggcg gtattcgctc tctgaacgtt gccctgcgcc aggaactgga tctctacatc
    25201 tgcctgcgtc cggtacgtta ctatcagggc actccaagcc cggttaaaca ccctgaactg
    25261 accgatatgg ttatcttccg tgaaaactcg gaagacattt atgcgggtat cgaatggaaa
    25321 gctgactctg ccgacgccga gaaagtgatt aaattcctgc gtgaagagat gggcgtgaag
    25381 aaaattcgct tcccggaaca ttgcggtatc ggtattaagc cgtgttctga agaaggcacc
    25441 aaacgtctgg ttcgtgcagc gatcgaatac gcaattgcta acgatcgtga ctctgtgacc
    25501 ctggtgcaca aaggcaacat catgaagttc accgaaggcg cgtttaaaga ctggggctac
    25561 cagctggcgc gtgaagaatt tggcggtgaa ctgatcgacg gcggcccgtg gctgaaagtt
    25621 aaaaacccga ataccggcaa agagatcgtc attaaagacg tgattgctga tgccttcctg
    25681 caacagatcc tgctgcgtcc ggctgaatat gatgttatcg cctgtatgaa cctgaacggt
    25741 gactacattt ctgacgccct ggcagcgcag gttggcggta tcggtatcgc ccctggtgca
    25801 aacatcggtg acgaatgcgc cctgtttgaa gccacccacg gtactgcgcc gaaatatgcc
    25861 ggtcaggaca aagttaaccc aggttctatt attctctctg ctgagatgat gttacgccac
    25921 atgggttgga ccgaagccgc agacctgatt gttaaaggta tggaaggcgc aatcaatgcg
    25981 aagaccgtaa cctatgactt cgaacgtctg atggaaggcg ctaaactgct gaaatgttca
    26041 gagtttggtg atgcgatcat caaaaatatg taatcactac atgtgttaaa tatttaaacg
    26101 ggcgta
//
LOCUS       DAPGRN010000065        26040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPGRN010000065 DAPGRN010000000
VERSION     DAPGRN010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.065"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            188..382
                     /gene="bfd"
                     /locus_tag="RHW33_002824"
     CDS             188..382
                     /gene="bfd"
                     /locus_tag="RHW33_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV1646308.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            455..931
                     /gene="bfr"
                     /locus_tag="RHW33_002825"
     CDS             455..931
                     /gene="bfr"
                     /locus_tag="RHW33_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV1646309.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(928..1395)
                     /locus_tag="RHW33_002826"
     CDS             complement(928..1395)
                     /locus_tag="RHW33_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV1646310.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1774..2085
                     /gene="rpsJ"
                     /locus_tag="RHW33_002827"
     CDS             1774..2085
                     /gene="rpsJ"
                     /locus_tag="RHW33_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV1646311.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2118..2747
                     /gene="rplC"
                     /locus_tag="RHW33_002828"
     CDS             2118..2747
                     /gene="rplC"
                     /locus_tag="RHW33_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV1646312.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2758..3363
                     /gene="rplD"
                     /locus_tag="RHW33_002829"
     CDS             2758..3363
                     /gene="rplD"
                     /locus_tag="RHW33_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV1646313.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3360..3662
                     /gene="rplW"
                     /locus_tag="RHW33_002830"
     CDS             3360..3662
                     /gene="rplW"
                     /locus_tag="RHW33_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV1646314.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3680..4501
                     /gene="rplB"
                     /locus_tag="RHW33_002831"
     CDS             3680..4501
                     /gene="rplB"
                     /locus_tag="RHW33_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV1646315.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4518..4796
                     /gene="rpsS"
                     /locus_tag="RHW33_002832"
     CDS             4518..4796
                     /gene="rpsS"
                     /locus_tag="RHW33_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV1646316.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4811..5143
                     /gene="rplV"
                     /locus_tag="RHW33_002833"
     CDS             4811..5143
                     /gene="rplV"
                     /locus_tag="RHW33_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV1646317.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5161..5862
                     /gene="rpsC"
                     /locus_tag="RHW33_002834"
     CDS             5161..5862
                     /gene="rpsC"
                     /locus_tag="RHW33_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV1646318.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5875..6285
                     /gene="rplP"
                     /locus_tag="RHW33_002835"
     CDS             5875..6285
                     /gene="rplP"
                     /locus_tag="RHW33_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV1646319.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6285..6476
                     /gene="rpmC"
                     /locus_tag="RHW33_002836"
     CDS             6285..6476
                     /gene="rpmC"
                     /locus_tag="RHW33_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV1646320.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6476..6730
                     /gene="rpsQ"
                     /locus_tag="RHW33_002837"
     CDS             6476..6730
                     /gene="rpsQ"
                     /locus_tag="RHW33_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV1646321.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6895..7266
                     /gene="rplN"
                     /locus_tag="RHW33_002838"
     CDS             6895..7266
                     /gene="rplN"
                     /locus_tag="RHW33_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV1646322.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7277..7591
                     /gene="rplX"
                     /locus_tag="RHW33_002839"
     CDS             7277..7591
                     /gene="rplX"
                     /locus_tag="RHW33_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV1646323.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7606..8145
                     /gene="rplE"
                     /locus_tag="RHW33_002840"
     CDS             7606..8145
                     /gene="rplE"
                     /locus_tag="RHW33_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV1646324.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8160..8465
                     /gene="rpsN"
                     /locus_tag="RHW33_002841"
     CDS             8160..8465
                     /gene="rpsN"
                     /locus_tag="RHW33_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV1646325.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8499..8891
                     /gene="rpsH"
                     /locus_tag="RHW33_002842"
     CDS             8499..8891
                     /gene="rpsH"
                     /locus_tag="RHW33_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV1646326.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8904..9437
                     /gene="rplF"
                     /locus_tag="RHW33_002843"
     CDS             8904..9437
                     /gene="rplF"
                     /locus_tag="RHW33_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV1646327.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9447..9800
                     /gene="rplR"
                     /locus_tag="RHW33_002844"
     CDS             9447..9800
                     /gene="rplR"
                     /locus_tag="RHW33_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV1646328.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9815..10318
                     /gene="rpsE"
                     /locus_tag="RHW33_002845"
     CDS             9815..10318
                     /gene="rpsE"
                     /locus_tag="RHW33_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV1646329.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10322..10501
                     /gene="rpmD"
                     /locus_tag="RHW33_002846"
     CDS             10322..10501
                     /gene="rpmD"
                     /locus_tag="RHW33_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV1646330.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10505..10939
                     /gene="rplO"
                     /locus_tag="RHW33_002847"
     CDS             10505..10939
                     /gene="rplO"
                     /locus_tag="RHW33_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV1646331.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10947..12278
                     /gene="secY"
                     /locus_tag="RHW33_002848"
     CDS             10947..12278
                     /gene="secY"
                     /locus_tag="RHW33_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV1646332.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12310..12426
                     /gene="rpmJ"
                     /locus_tag="RHW33_002849"
     CDS             12310..12426
                     /gene="rpmJ"
                     /locus_tag="RHW33_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV1646333.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12573..12929
                     /gene="rpsM"
                     /locus_tag="RHW33_002850"
     CDS             12573..12929
                     /gene="rpsM"
                     /locus_tag="RHW33_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV1646334.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12946..13335
                     /gene="rpsK"
                     /locus_tag="RHW33_002851"
     CDS             12946..13335
                     /gene="rpsK"
                     /locus_tag="RHW33_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV1646335.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13369..13989
                     /gene="rpsD"
                     /locus_tag="RHW33_002852"
     CDS             13369..13989
                     /gene="rpsD"
                     /locus_tag="RHW33_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV1646336.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14015..15004
                     /gene="rpoA"
                     /locus_tag="RHW33_002853"
     CDS             14015..15004
                     /gene="rpoA"
                     /locus_tag="RHW33_002853"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV1646337.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15045..15428
                     /gene="rplQ"
                     /locus_tag="RHW33_002854"
     CDS             15045..15428
                     /gene="rplQ"
                     /locus_tag="RHW33_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV1646338.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15535..15903
                     /locus_tag="RHW33_002855"
     CDS             15535..15903
                     /locus_tag="RHW33_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV1646339.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15914..16339
                     /gene="zntR"
                     /locus_tag="RHW33_002856"
     CDS             15914..16339
                     /gene="zntR"
                     /locus_tag="RHW33_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV1646340.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16395..16613
                     /gene="arfA"
                     /locus_tag="RHW33_002857"
     CDS             16395..16613
                     /gene="arfA"
                     /locus_tag="RHW33_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV1646341.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNDFFTTGLLLSGVC"
     gene            complement(16610..17020)
                     /gene="mscL"
                     /locus_tag="RHW33_002858"
     CDS             complement(16610..17020)
                     /gene="mscL"
                     /locus_tag="RHW33_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV1646342.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17150..18526)
                     /gene="trkA"
                     /locus_tag="RHW33_002859"
     CDS             complement(17150..18526)
                     /gene="trkA"
                     /locus_tag="RHW33_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV1646343.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18548..19837)
                     /gene="rsmB"
                     /locus_tag="RHW33_002860"
     CDS             complement(18548..19837)
                     /gene="rsmB"
                     /locus_tag="RHW33_002860"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV1646344.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19883..20830)
                     /gene="fmt"
                     /locus_tag="RHW33_002861"
     CDS             complement(19883..20830)
                     /gene="fmt"
                     /locus_tag="RHW33_002861"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV1646345.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWVEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20845..21354)
                     /gene="def"
                     /locus_tag="RHW33_002862"
     CDS             complement(20845..21354)
                     /gene="def"
                     /locus_tag="RHW33_002862"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV1646346.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21484..22608
                     /gene="dprA"
                     /locus_tag="RHW33_002863"
     CDS             21484..22608
                     /gene="dprA"
                     /locus_tag="RHW33_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV1646347.1"
                     /translation="MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22580..23053
                     /locus_tag="RHW33_002864"
     CDS             22580..23053
                     /locus_tag="RHW33_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV1646348.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23082..23624
                     /locus_tag="RHW33_002865"
     CDS             23082..23624
                     /locus_tag="RHW33_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV1646349.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23629..24201
                     /gene="tsaC"
                     /locus_tag="RHW33_002866"
     CDS             23629..24201
                     /gene="tsaC"
                     /locus_tag="RHW33_002866"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV1646350.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24206..25024
                     /gene="aroE"
                     /locus_tag="RHW33_002867"
     CDS             24206..25024
                     /gene="aroE"
                     /locus_tag="RHW33_002867"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV1646351.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFV
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQTLGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRTADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25021..25278
                     /locus_tag="RHW33_002868"
     CDS             25021..25278
                     /locus_tag="RHW33_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV1646352.1"
                     /translation="MNQAIQFPDREEWVENKKCVCFPALVNGMQLTCAISGDSLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25254..25808)
                     /locus_tag="RHW33_002869"
     CDS             complement(25254..25808)
                     /locus_tag="RHW33_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV1646353.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6684 a         6172 c         6751 g         6433 t
ORIGIN      
        1 gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc tttttgctgc
       61 ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat ggcgcaaata
      121 aaactgtgct attgtaatca taactattct catttacact ttgcgcagaa tttgaacggg
      181 agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc gccaggcagt
      241 ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg tgggaaatca
      301 atgtggtaag tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga tgcagatgcc
      361 agaatttaaa gagattgcct gaagcccacg cttatttttt gacatcccgc tagccccatc
      421 tacgctctaa tagtggaagc ggagggacta taaaatgaaa ggtgatgtta aaatcataaa
      481 ttatctcaat aaactattgg gaaatgagct tgtcgcaatc aatcagtatt ttctccatgc
      541 ccggatgttt aaaaactggg gactgatgcg tcttaatgac gtcgaatacc atgaatccat
      601 cgatgagatg aaacacgctg ataaatatat cgagcgtatt ttgtttttag aaggaatccc
      661 caacttacag gatttaggta agctagggat cggtgaagat gtcgaagaga tgctgcaatc
      721 cgacctgcga ttagagctgg aaggtgcgaa ggatctgcgt gaggctatcg cgtatgccga
      781 cagcgttcat gactatgtta gccgcgatat gatgatcgag atccttgccg atgaagaagg
      841 tcatatcgac tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc tacaaaatta
      901 cctgcaatca caaattaaag ttaaagatta acggcctatc agcaaactat cccagcctat
      961 aacgaaaccc gcagccgcca gaaatggccc aaaaggcagc gggtttttta atgattcttt
     1021 tcctctcatg agcagaccaa taactacggc tccgcaagcg aatgaggcag caaggaaaac
     1081 cagtcgtggt aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa acttcacatc
     1141 accatagcct aatccttctt tatggcgcag tatgcgatac ccccagtaaa tgacagcgaa
     1201 tgtgccgtaa ccaataattg ccccccataa cgcatcggct aaaccatcag gatgacaaac
     1261 ttgatagaac aaaagacctg accagagtaa cgggcacgta aatctgtcag gtaacaagcc
     1321 atgctttgca tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa ttagaaaggg
     1381 aagggtagtg tccataaaac atcctctgta attggagtat caccatcgaa taactccgga
     1441 tgctcagcaa cagagagagc gctgatttgt taacctgatt ccggtgaaaa acgctatctt
     1501 cacaacctta caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta
     1561 cccgatgctt gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg tagttgacag
     1621 caggttcaat ctgaacctca gttagccgaa tttggctacc taacaatgct cccaatcggg
     1681 gagctacgta agaacggtta cactctccca tcaatcgtaa tgggtctgag gagtaatcat
     1741 tttcgtttat aaaataattg gagctctggt ctcatgcaga accaaagaat ccgtatccgc
     1801 ctgaaagcgt ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt cgagactgcc
     1861 aagcgcactg gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc
     1921 actgttctga tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact
     1981 cacttgcgtc tggttgacat cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt
     2041 ctggatctgg ctgccggtgt agacgtgcag atcagcctgg gttaatcagg tcattgagcg
     2101 attgagaggt tgaaacaatg attggtttag tcggtaaaaa agtgggtatg acccgtatct
     2161 tcacagaaga cggcgtttct atcccagtaa ccgtaatcga agttgaagca aaccgcgtta
     2221 ctcaggttaa agacctggct aacgatggct accgtgctat tcaggtgacc accggtgcta
     2281 aaaaagctaa ccgtgtgacc aagcctgaag ctggccactt cgctaaagct ggcgtagaag
     2341 ctggccgtgg tctgtgggaa ttccgcctgg ctgaaggcga agagttcact gtaggtcaga
     2401 gcattagcgt tgaactgttt gctgacgtta aaaaagttga cgtaactggc acctctaaag
     2461 gtaaaggttt cgcaggtacc gttaagcgct ggaacttccg tacccaggac gctactcacg
     2521 gtaactcctt gtctcaccgc gttccgggtt ctatcggtca gaaccagact ccgggcaaag
     2581 tgttcaaagg caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc
     2641 ttgacgtagt acgcgttgac gctgagcgca acctgctgct ggttaaaggt gctgtcccgg
     2701 gtgcaaccgg tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg
     2761 gaattagtat tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt
     2821 gatttcaacg aagcgctggt tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag
     2881 ggtactcgtg ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc
     2941 cagaaaggca ccggccgtgc gcgttctggt tctatcaaga gcccgatctg gcgttctggt
     3001 ggcgtgacct ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa gaagatgtac
     3061 cgcggcgcgc tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc
     3121 gagaagttct ctgtagaagc gccgaaaact aagctgctgg cacagaaact gaaagacatg
     3181 gctctggaag atgtgctgat catcaccggt gagctggacg aaaacctgtt cctggctgcg
     3241 cgcaacctgc acaaggttga cgtacgcgat gcaactggta tcgacccggt tagcctgatc
     3301 gccttcgaca aagtcgtaat gactgctgat gctgttaagc aagttgagga gatgctggca
     3361 tgattcgtga agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt
     3421 ctactgcgat ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac gcgaccaaag
     3481 cagaaatcaa agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc
     3541 tggtagttaa agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga
     3601 aaaaagctta cgtcaccctg aaagaaggcc agaatctgga cttcgttggc ggcgctgagt
     3661 aagtcggagg agtaatacaa tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg
     3721 ccacgtagtt aaagtggtta accctgagct gcacaagggc aaaccttttg ctccgttgct
     3781 ggaaaaaaac agcaaatccg gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat
     3841 cggtggtggc cacaagcagg cttaccgtat tgttgacttc aaacgcaaca aagacggtat
     3901 cccggcagtt gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt
     3961 tctgtacaaa gacggtgaac gccgttacat cctggcccct aaaggcctga aagctggcga
     4021 ccagattcag tctggcgttg atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa
     4081 catcccggtt ggttctactg ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct
     4141 ggcacgttcc gctggtactt acgttcagat cgttgctcgt gatggtgctt atgtcaccct
     4201 gcgtctgcgt tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga
     4261 agttggcaat gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg
     4321 tggtgttcgt ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc cacatggtgg
     4381 tggtgaaggt cgtaactttg gtaagcaccc ggtaactccg tggggcgttc agaccaaagg
     4441 taagaagacc cgcagcaaca agcgtactga taaattcatc gtacgtcgcc gtagcaaata
     4501 attttagagg ataagccatg ccacgttctc tcaagaaagg tccttttatt gacctgcact
     4561 tgctgaagaa ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg cgcacttggt
     4621 cccgtcgttc aacgatcttt cctaacatga tcggtttgac catcgctgtc cataatggtc
     4681 gtcagcacgt tccggtattt gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg
     4741 caccgactcg tacttatcgc ggccacgctg ctgataaaaa agcgaagaag aaataaggta
     4801 ggaggaagag atggaaacta tcgctaaaca tcgccatgct cgttcttctg ctcagaaggt
     4861 tcgccttgtt gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg atattttgac
     4921 ctacaccaac aagaaagcgg ctgtactggt caagaaagtt ctggaatctg ccattgctaa
     4981 cgctgaacac aacgatggcg ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga
     5041 cgaaggcccg agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag atcgcatcct
     5101 gaagcgcacc agccacatca ctgtggttgt gtccgatcgc tgagactctg gagactagca
     5161 atgggtcaga aagtacatcc taatggtatt cgcctgggta ttgtaaaacc atggaactct
     5221 acctggtttg cgaacaccaa agaattcgct gacaacctgg acagcgattt taaagtacgt
     5281 cagtacctga ctaaggaact ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg
     5341 gctaagagca tccgtgtaac cattcacact gctcgcccgg gtatcgttat cggtaaaaaa
     5401 ggtgaagacg tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag
     5461 atcaacatcg ccgaagttcg taagcctgaa ctggacgcaa aactggttgc tgacagcatc
     5521 acttctcagc tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac
     5581 gcaatgcgtc tgggcgctaa aggtattaaa gttgaagtta gcggccgtct gggcggcgcg
     5641 gaaatcgcac gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct
     5701 gacatcgact acaacacctc tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg
     5761 tggatcttca aaggcgagat cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg
     5821 gctgctcagc ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta
     5881 caaccaaagc gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag
     5941 ggtacggatg ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact
     6001 gcccgtcaga tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag
     6061 atctggatcc gtgtgttccc ggacaaaccg atcactgaaa agccgctggc agtgcgtatg
     6121 ggtaaaggta aaggtaacgt ggagtattgg gttgccttga ttcagccggg taaagtcctg
     6181 tatgaaatgg acggtgttcc ggaagagctg gcccgtgaag cattcaagct ggcagcagcg
     6241 aaactgccga ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc
     6301 tgcgtgagaa gagcgttgaa gagctgaaca ccgagctgct gaacctgctg cgtgagcagt
     6361 tcaacctgcg tatgcaggct gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag
     6421 tgcgtcgcga tgtcgcacgc gttaagactt tactgaacga gaaggcgggt gcgtaatgac
     6481 cgataaaatc cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga aatccattgt
     6541 tgttgctatc gaacgttttg tgaaacaccc gatctacggt aaattcatca agcgtacgac
     6601 caaactgcac gtacatgacg agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg
     6661 cgaatgccgt ccgctgtcca agactaaatc ctggacgctg gttcgcgttg tagagaaagc
     6721 ggttctgtaa tacagtacac tctctcgata cgaataaacg gctcagaaat gagccgttta
     6781 ttttttctac ccatatcctt gaagcggtgt tataatgccg cgccctcgat atggggattt
     6841 ttaacgacct gattttcggg tctcagtagt agttgacatt agcggagcac taaaatgatc
     6901 caagaacaga ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc
     6961 aaggttctgg gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat caagatcacc
     7021 atcaaagaag caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg
     7081 gtgcgcacca agaagggtgt tcgtcgcccg gacggttctg tcattcgctt cgatggtaat
     7141 gcttgtgttc ttctgaacaa caacagcgag cagcctatcg gtacgcgtat ttttgggccg
     7201 gtaactcgtg agcttcgtag tgagaagttc atgaaaatta tctctctggc accagaagta
     7261 ctctaaggag cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac
     7321 cggtaaagat aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat
     7381 tgttgaaggt atcaacctgg ttaagaaaca tcagaagccg gttccggccc tgaaccaacc
     7441 gggtggcatc gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc
     7501 ggcaaccggc aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt
     7561 cttcaagtct aacagcgaaa ctatcaagta atttggagta gtacgatggc gaaactgcat
     7621 gattactaca aagacgaagt agttaaaaaa ctcatgactg agtttaacta caattctgtc
     7681 atgcaagtcc ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga agcgatcgct
     7741 gacaaaaaac tgctggataa cgcagcagca gacctggcag caatctccgg tcaaaaaccg
     7801 ctgatcacca aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc
     7861 ggctgtaaag taactctgcg tggcgaacgc atgtgggagt tctttgagcg cctgatcact
     7921 attgctgtac ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt
     7981 ggtaactaca gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga ctacgataaa
     8041 gtcgaccgcg ttcgtggttt ggatattacc attaccacta ctgcgaaatc tgacgaagaa
     8101 ggccgcgctc tgctggctgc ctttgacttc ccgttccgca agtaaggtag ggttactaaa
     8161 tggctaagca atcaatgaaa gcacgcgaag taaaacgcgt agctttagct gataaatact
     8221 tcgcgaaacg cgctgaactg aaagcgatca tctctgatgt gaacgcttcc gacgaagatc
     8281 gttggaacgc tgttctcaag ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc
     8341 gtaaccgctg ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc gggttgagcc
     8401 gtattaaggt ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct
     8461 ggtaattgtc accaattgaa tcacgggagt aaagacagat gagcatgcaa gatccgatcg
     8521 cggatatgct gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc
     8581 cttcctccaa gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag
     8641 attttaaagt tgaaggcgac accaagcctg aactggaact tactctgaag tatttccagg
     8701 gcaaagctgt tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc atctataaac
     8761 gtaaagatga gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt tctacctcta
     8821 aaggtgttat gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa attatctgct
     8881 acgtagccta atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg
     8941 ccggcgttga cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa aacggcgagc
     9001 tgactcgtac tctcaacgat gctgttgaag ttaaacatgc agataatacc ctgaccttcg
     9061 gtccgcgtga tggttacgca gacggttggg cacaggctgg taccgcgcgt gccctgctga
     9121 actcaatggt tatcggtgtt accgaaggct tcactaagaa gctgcagctg gttggtgtag
     9181 gttaccgtgc agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc tctcatcctg
     9241 ttgaccatca gctgcctgcg ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc
     9301 tgaaaggcgc tgataagcag gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc
     9361 gtcctgagcc ttataaaggc aagggtgttc gttacgccga cgaagtcgtg cgtaccaaag
     9421 aggctaagaa gaagtaaggt aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga
     9481 cccgcgcacg ccgcaagctc caggagctgg gcgcaactcg cctggtggta catcgtaccc
     9541 cgcgtcacat ttacgcacag gtaattgcac cgaacggttc tgaagttctg gtagctgctt
     9601 ctactgtaga aaaagctatc gctgaacaac tgaagtacac cggtaacaaa gacgcggctg
     9661 cagctgtggg taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct
     9721 ttgaccgttc cgggttccaa tatcatggtc gtgtccaggc actggcagat gctgcccgtg
     9781 aagctggcct tcagttctaa ggtagaggtg taagatggct cacatcgaaa aacaagctgg
     9841 cgaactgcag gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg
     9901 tattttctcc ttcacagctc tgactgtagt tggcgatggt aacggtcgcg ttggttttgg
     9961 ttacggtaaa gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg
    10021 caatatgatt aacgtcgcgc tgaataacgg cactctgcaa caccctgtta aaggtgttca
    10081 cacgggttct cgcgtattca tgcagccggc ttccgaaggt accggtatca tcgccggtgg
    10141 tgcaatgcgc gccgttctgg aagtcgctgg ggttcataac gttctggcta aagcatatgg
    10201 ttccaccaac ccgatcaacg tggttcgtgc aactattgat ggcctggaaa atatgaattc
    10261 tccagaaatg gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg ggaaataaac
    10321 catggcaaag actattaaaa ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca
    10381 caaggcaacg ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag agcgcgagga
    10441 tactcctgct attcgcggta tgatcaacgc ggtttccttc atggttaaag ttgaggagta
    10501 agagatgcgt ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct
    10561 gggtcgtggt atcggttctg gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa
    10621 gtctcgttct ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg
    10681 tcgtctgccg aaattcggct tcacttctcg taaagcagcg attacagccg aagttcgtct
    10741 gtctgacctg gctaaagtag aaggcggtgt agtagacctg aacacgctga aagcggctaa
    10801 cattatcggt atccagatcg agttcgcgaa agtgatcctg gctggcgaag taacgactcc
    10861 ggtaactgtt cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg aagctgctgg
    10921 cggtaaaatc gaggaataag tagcagatgg ctaaacaacc gggattagat tttcaaagtg
    10981 ccaaaggtgg cttaggcgag ctgaaacgca gactgctgtt tgttatcggt gcgctgattg
    11041 tgttccgtat tggctctttt attccgatcc ctggtattga tgccgctgta cttgccaaac
    11101 tgcttgagca acagcgaggc accatcattg agatgtttaa catgttctct ggtggtgctc
    11161 tcagccgtgc ttctatcttt gctctgggga tcatgccgta tatttcggcg tcgatcatta
    11221 tccagctgct gacggtggtt cacccaacgt tggcagaaat taagaaagaa ggggagtctg
    11281 gtcgtcgtaa gatcagccag tacacccgct acggtactct ggtgctggca atattccagt
    11341 cgatcggtat tgctaccggt ctgccgaata tgcctggtat gcaaggcctg gtgattaacc
    11401 cgggctttgc attctacttc accgctgttg taagtctggt cacaggaacg atgttcctga
    11461 tgtggttggg cgaacagatc actgaacgag gtatcggcaa cggtatttca atcattatct
    11521 tcgccggtat tgtcgcggga ctcccgccag ccattgccca tactatcgag caagcgcgtc
    11581 aaggcgacct gcacttcctc gtgttgctgt tggttgcagt attagtattt gcagtgacgt
    11641 tctttgttgt atttgttgag cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc
    11701 agcaaggtcg tcgtgtctat gctgcacaga gcacacattt accgctgaaa gtgaatatgg
    11761 cgggggtaat cccggcaatc ttcgcttcca gtattattct gttcccggcg accatcgcgt
    11821 catggttcgg gggcggtact ggttggaact ggctgacaac aatttcgctg tatttgcagc
    11881 ctgggcaacc gctttatgtg ttactctatg cgtctgcaat catcttcttc tgtttcttct
    11941 acacggcgtt ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat
    12001 ttgtaccagg aattcgtccg ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc
    12061 gcctgaccct ggttggtgcg ctgtatatta cctttatctg cctgatcccg gagttcatgc
    12121 gtgatgcaat gaaagtaccg ttctacttcg gtgggacctc actgcttatc gttgttgtcg
    12181 tgattatgga ctttatggct caagtgcaaa ctctgatgat gtccagtcag tatgagtctg
    12241 cattgaagaa ggcgaacctg aaaggctacg gccgataatt ggtcgcccga gaagttacgg
    12301 agagtaaaaa tgaaagttcg tgcttccgtc aagaaattat gccgtaactg caaaatcgtt
    12361 aagcgtgatg gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa acagcgccaa
    12421 ggctgatttt ttcgcatatt tttcttgcaa agttgggttg agctggctag attagccagc
    12481 caatcttttg tatgtctgtg cgtttccatt tgagtatcct gaaaacgggc ttttcagcat
    12541 ggaacgtaca tattaaatag taggagtgca tagtggcccg tatagcaggc attaacattc
    12601 ctgatcataa gcatgccgta atcgcattaa cttcgattta tggcgtcggc aagacccgtt
    12661 ctaaagccat cctggctgca gcgggtatcg ctgaagatgt taagatcagt gagctgtctg
    12721 aaggacaaat cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc
    12781 gccgtgaaat cagcatgagc atcaagcgcc tgatggatct tggttgctat cgcggtttgc
    12841 gtcatcgtcg tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta
    12901 agggtccgcg caaaccgatc aagaaataat cggggtgatt gaataatggc aaaggcacca
    12961 attcgtgcac gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca tatccatgct
    13021 tctttcaaca acaccatcgt gactatcact gatcgtcagg gtaacgcgtt gggttgggca
    13081 acagccggtg gttccggttt ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt
    13141 gcagcagagc gttgcgctga cgccgtgaaa gaatacggca tcaagaatct ggaagttatg
    13201 gttaaaggtc cgggtccagg ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc
    13261 cgcatcacta acattactga tgtgactccg atccctcata acggttgtcg tccgccgaaa
    13321 aaacgtcgcg tataacgcct cgttttccag gtttgttgga gaaagaaaat ggcaagatat
    13381 ttgggtccta agctcaagct gagccgtcgt gagggcaccg acttattcct taagtctggc
    13441 gttcgcgcga tcgataccaa gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt
    13501 aaaccgcgtc tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc
    13561 tatggtgtgc tggagcgtca gttccgtaac tactacaaag aagcagcacg tctgaaaggc
    13621 aacaccggtg aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt
    13681 atgggcttcg gtgccactcg tgcagaagca cgtcagctgg ttagccataa agcaattatg
    13741 gtaaacggtc gtgttgttaa catcgcttct tatcaggtta gtccgaatga cgttgtaagc
    13801 attcgtgaga aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct ggctgagcag
    13861 cgtgaaaagc caacctggct ggaagttgat gctggcaaga tggaaggtac gtttaagcgt
    13921 aagccggagc gttctgatct gtctgcggac attaacgaac acctgatcgt cgagctttac
    13981 tccaagtaaa gcttagtacc aaagagagga cacaatgcag ggttctgtga cagagtttct
    14041 aaaaccgcgc ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga
    14101 gcctttagag cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc
    14161 atcgatgccg ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag
    14221 caccaaagaa ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc
    14281 ggtgagagtt cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc
    14341 tgtgactgca gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt
    14401 gatctgccac ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg
    14461 tcgtggttat gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg
    14521 ccgtctgctg gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc
    14581 agcgcgtgta gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg
    14641 cacaatcgat cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga
    14701 agctttcgtt gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagagtt
    14761 cgatccgatc ctgctgcgcc ctgttgacga tctggaattg actgtccgct ctgctaactg
    14821 ccttaaagca gaagctatcc actatatcgg tgatctggta cagcgtaccg aggttgagct
    14881 ccttaaaacg cctaaccttg gtaaaaaatc tcttactgag attaaagacg tgctggcttc
    14941 ccgtggactg tctctgggca tgcgcctgga aaactggcca ccggcaagca tcgctgacga
    15001 gtaaccggat cacaggttaa ggttttactg agaaggataa ggtcatgcgc catcgtaaga
    15061 gtggtcgtca actgaaccgc aacagcagcc atcgccaggc tatgttccgc aatatggcag
    15121 gttcactggt tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa gagctgcgcc
    15181 gcgtagttga gccgctgatt actcttgcca agactgatag cgttgctaat cgtcgtctgg
    15241 cattcgcccg tactcgtgat aacgagatcg tggcaaaact gtttaacgaa ctgggcccgc
    15301 gtttcgcgag ccgtgccggt ggttacactc gtattctgaa gtgtggcttc cgtgcaggcg
    15361 acaacgcgcc gatggcttat atcgagctgg ttgatcgttc agagaaagca gaagctgctg
    15421 cagagtaatc tgaagcaacg taaaaaaacc cgccccggcg ggttttttta tacccgtagc
    15481 atccccactt atctacaata gctgtactct ttttgttcat cccctggagt atttatgtgg
    15541 ttacttgacc agtgggcaga gcgccatata gcagaagcgc aagcgaaagg tgagtttgat
    15601 aacctggcag gtagcggcga accattgata ctggatgatg attctcatgt gccaccggaa
    15661 ttacgtgcgg ggtatcgctt gctgaagaat gccggttgct taccgccaga acttgagcaa
    15721 cggagggaag caattcagct tctggatatc ctcaaaggta tccgtcacga tgatccgcaa
    15781 tatcaagagg ttagccgtcg attgtcatta ctggaattga agctgcgaca agctggattg
    15841 agtaccgatt ttttacgcgg cgattatgct gacaagttgt tgaataaaat caacgataac
    15901 tagtggagta tgtatgtatc gcattggtga gctggcaaaa atggcggaag taacacccga
    15961 cacgattcgt tattacgaaa agcagcagat gatggagcat gaagtgcgta ctgaaggtgg
    16021 gtttcgcctg tataccgaaa gcgatctcca gcgattgaaa tttatccgcc atgccagaca
    16081 actaggtttc agtctagagt cgatccgcga gttgctgtcg atccgtatcg atcctgaaca
    16141 ccatacctgt caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag tcgaagcacg
    16201 gatagccgag ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg cctgttgtgg
    16261 gaccgcccat agcagtgttt attgctcgat tcttgaagct cttgaacaag gggcgagtgg
    16321 cgttaagagt ggctgttgat tttttgcact gacagaatta cactcgcgcc gttaaataac
    16381 caactggagt ttttatgagc cgatatcagc atactaaagg gcagataaag gataatgcga
    16441 tagaagcatt actacatgat cccttattcc gacagcgagt agagaaaaat aagaaaggga
    16501 aaggcagtta catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag gccagtggca
    16561 agaaagtaaa tgactttttt accactggcc ttctgctttc aggcgtttgt taagagcggt
    16621 tattctgctc tttcagcaaa tcacgaattt ctgtcagtaa tacttcttct ttagttggtg
    16681 ctggtgcggc tgctggttct tcttttttcc gattcagttt gttgattagc ttaatcgcca
    16741 taaagatggc aaaggccaca atcagaaaat caaagacgtt ttgaatgaag acaccgtaat
    16801 gcatcacaac agcagggata tccccctgcg catcgcgtag cgtgacagca aactgtttaa
    16861 agtcgatccc gccaattaat aagcccagag gtggcatgat gatatcggca accagtgaag
    16921 atacaatctt cccgaatgcc gcaccgataa tgacacccac cgccaaatcc accacgttcc
    16981 cgcgcatcgc aaattcgcga aattctttaa taatgctcat gttattctcc ctatgaagcc
    17041 gacaaccata agtctaacaa atgttaagcc attttcctgc caggaatgaa tttaattaat
    17101 ccttgataat caaagggaaa taataaggcg tcattagacg ccttattaat tacaagaaga
    17161 aagggcttgg ctggaagagt ctttcgacgt cggtaataaa ttttttatct gtgaggaaca
    17221 taattacgtg atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc acgtcgtttc
    17281 cacgtaccac cgctccaata atcgttcctg gcggtagctt gatttcgtca atgactctgc
    17341 cgacaacgcg tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct tctgctacgc
    17401 cgcggcgcaa tgaggaaaca ccaacaatat ctgcttttcg cacatggcta agcaacgcag
    17461 aaatagttgc ttgttgtggt gaaatcgcaa tatcgatcac gctcccctga accagatcca
    17521 cataagcgcg acgctggatc aataccatca cctttttcgc acccatacgt ttggcaagca
    17581 tggcggacat gatattggcc tcgtcatcgt tggtgacggc aataaacaga tcaacttgat
    17641 cgatatgttc ttcggccagc agttcttgat ccgacgcatc accaaaaaag acgatcgtat
    17701 tctgcaactt ttccgccagt tcggcagcgc gctgctgatt acgttcgatg agtttaacgc
    17761 tgtaatcttt ttccagacga cgcgccagcc ctgcaccgat attaccgcca ccaaccagca
    17821 tgatccgctt atacggtttt tccagtcgct gtaattcact catcaccgcg cggatatgct
    17881 gtgaagcggc aataaagaac acttcatcac cagcctcaac aatggtcgaa ccttgcggac
    17941 gaatggggcg atcgtggcgg aaaattgctg ccacacgagt atcgatatgt ggcatatgtt
    18001 cgcgcatggt cgaaagtgca ttaccaatca gcgggccgcc ataataggct ttaaccacag
    18061 ccaggctgac tttaccctca gcgaagttca ccacctgcaa tgcgccggga tactcaatca
    18121 gtcggtaaat attatcgata accaactgct ctggtgcgat cagatgatca atcggtacag
    18181 catctgaatg aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg atacgagcga
    18241 tgcgattagg ggtgttgaaa agtgagtagg ctacctggca ggcaaccata ttggtttcat
    18301 ctgaactggt tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc cgcaatacgc
    18361 gtggatgaga gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc aaggtccgca
    18421 gacgctcacc gttggtatcg acaacagtga tatcgttgtt ctcgccaacc aggttttccg
    18481 ccagtgtgcc gccaacctgg ccggcaccca gaatgataat tttcatcagt cgcgacccgt
    18541 tatctcatca ctttttgatt agcttagcgt aaaagaagcc gtcgccctct tcggcaccag
    18601 gcagattttg tttacccggt tgctctggtg ttcctgtttc gcaaagttcg gcatcagcgg
    18661 tacgttgcaa aaaggcttta atctgcaggc tattctcttc cggtaacacc gaacaggtgg
    18721 catagaccag ggttccaccg gattttaaat gcggccaaat ggcgtcgaga atttcagact
    18781 gcaattgcgc gagttccggg atatcgcgat cgcgacgtaa ccatttaata tctggatggc
    18841 gacgaatcac accggttgct gaacaaggcg catctaataa aatgcgatca aactgttgct
    18901 cgccacacca ttgagaaggg taacggccat caccttgttt cacggtcgcc ttcataccaa
    18961 ggcgttttaa gttgtcgtaa acgcgagaga ggcgctgttc gtcaatatca accgcaagaa
    19021 cctgcgcttc tggtgccacc tcaaggatat gcgttgtttt accgccgggg gcggcgcaaa
    19081 gatccagaat gtgttcaccg ttttgtggcg caagccaggt catgcaacct tgtgctgatg
    19141 catcctgaac ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca ggtgcaggtg
    19201 tttccagccg tacagcatca gggtaatccg catgcgggaa acctttcatt cctgcttcat
    19261 ccagcaatgc aagccagctg tcgcgggaat gatgcgtacg attaatacgc agccacattg
    19321 gcggacgctg gttattggct tcgacgatgg attgccactg ctctggatac gctttttgca
    19381 gacgcttcag caaccaggaa ggatgcagat aacgtgcatc actggtatta aactcggcta
    19441 ataactcttc ttgctgacgc tggaactggc gtaatacacc gttaatcaac cctttaagtt
    19501 gcggacgctt aattgcgata gcgccttcaa ccgtttcagc cagcgcagca tgaggtggaa
    19561 tgcgggtata aagcagttga tacaaaccaa ccataatcag gtaatgcaca gtgcgctgtt
    19621 tgccggtcat cggacgggcc attaacttat taatcagcca gtctaactgc gaaagcgtac
    19681 gcagtacgcc aaagcacaac tcttgaagaa gtgctttgtc tttatcggaa actttttgct
    19741 ggagcggtgg cagaatgttg cttaatgatt gcccttgctc gacgacttgt tcaacggcct
    19801 gggccgccat gctacgtaaa ttacgttgtt ttttcataag tataaaaatg cccggcaaga
    19861 ccgggcttag aagagtggac tatcagacca gacggttgcc cggaacaaac cattcccgac
    19921 gagagttcag gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt aacgagagca
    19981 ggttcaggat gccatcaccc gtcgcaacct gaatgccttg tttgttggct tcaaggatcg
    20041 ttcctggtgc agcgttggtt gccgtatcaa tgaccgatgc tttccagact ttaaccggct
    20101 gtccttcaat ttctacccag ctcattggcc atggattgaa agcgcgaatg cagcgttcaa
    20161 gctgtgctgc cgaaagtgac cagtcaatac gcgcttcttc tttactcaac ttctcggcgt
    20221 aagtgacaag agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc aattgtttca
    20281 acgtggtgat aagcccttgt gggccaagct ctgccagctt gtcgtacagc gtaccactgg
    20341 tatcttctgc agtaatcggg caggagagct tatagagcat gtcaccggtg tctaaaccaa
    20401 catccatttg cataatggtc acaccagttt ctgcatcacc cgcccatagt gagcgttgga
    20461 ttggtgcagc accgcgccag cgtggcagca gtgaaccatg aacgttgata cagccaagac
    20521 gcggcatctc cagcactgct ttaggcagaa ttaagccata ggcgacgaca accataacat
    20581 cagcctgtag atcggcgacc agttgctggt tttcttgtgg acgcagggaa acaggttgaa
    20641 aaacgggcag acctttttcc tcagccagaa ctttaaccgg gctgggcatc agttttttac
    20701 cgcgtcctgc cggtcggtct ggctgggtga acacgccaac gacgttatga ccagaagaca
    20761 acagcgcgtc gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata atacgtagtg
    20821 attctgacac gttagttctt atccttaagc ccgggctttc agacgatcca gtttttcaac
    20881 tttctgacga atacgttgtt gtttcagcgg tgacagataa tccataaaca gtttgccgac
    20941 caggtgatcc atctcatgct gaatacagat ggctaacaga ccatctgctt ccagttcaaa
    21001 tggtttaccg tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg gcactaaagc
    21061 tcgttgttca gggatcgaca ggcatccttc ttcaatgcct gtttcgccgc ttttttctaa
    21121 aagctccgga ttgattaaca ccagccgttc gtcacggttt tccgaaacat caataacaat
    21181 gatacgttga tggatatcaa cctgggttgc cgccaggcca ataccttctt ctgcgtacat
    21241 cgtctcgaac atatcatcga cgatacgctg aatttctgca ttcacttctt ctaccggttt
    21301 agcaactttg cgaagccgct cgtccggaat atgtaacact tgcaaaactg acataaatct
    21361 ccagagatgt gttcaggagt tagaaagatt atttcttcta ttctagacaa atccccctct
    21421 gattgacagc atcactgacc aatcgcaaag attgctaagg ctgcttatgg cagggagata
    21481 aggatggtcg atacagaaat ttggctgcgt ttaatgagcg tcagcagctt gtacggcgat
    21541 gatatggtcc gtatcgctca ctggctggca aaacagtcgc atattgatgc ggttgtattg
    21601 cagcaaacag ggcttacatt gcggcaggca caacgctttc tttcatttcc acgaaagagt
    21661 atcgaaagct cactttgttg gttggagcaa cccaaccatc atttaatccc tgcggacagc
    21721 gaattttatc ctcctcaact tctggcgacg acagattacc ccggcgcact gtttgttgaa
    21781 ggagaactgc acgcgctgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca
    21841 tggtatggcg agcgatgggg acgattattt tgcgaaactc tggcgaagcg tggagtgaca
    21901 attacgagtg gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc cttacaggta
    21961 aatggcgtca gcattgctgt attggggaat ggacttaata ccattcatcc gcgtcgccat
    22021 gcccgactgg ctaccagttt gcttgaacat ggtggggcac ttgtctcgga atttcccctc
    22081 gatgttccac cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt
    22141 aaaggtgtac tggtggtgga agcggctttg cgcagtggtt cgctggtgac agcacgttgt
    22201 gcgcttgagc aggggcgtga agtttttgcc ctgccaggtc cattagggaa tccgggaagc
    22261 gaagggcctc actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt
    22321 ctggaaaact tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat
    22381 tcaccagatc aggaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat
    22441 gaggtaacac ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagtt
    22501 actcaactac tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc
    22561 cgattgagga gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca
    22621 cacagaagct gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg
    22681 atttgatcga gaagatatct acaatgccct gctttggctg gaaaagcttg cggattatca
    22741 ggaaggactg gcagaaccga tgcaactggc ctctgatcca ctctccatgc gtatttatac
    22801 ccctgaggag tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt tccttgagca
    22861 aattcaggtg cttaaccttg aaacccgtga aatggtgata gagcgagtgc tcgcgctgga
    22921 taccgcagag ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt tgttcaatat
    22981 tccgggctgc gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg
    23041 tatgctgcat taatcatttc tttaattcag cataagttgt tatggcgaaa tcagcactgt
    23101 tcacggtgcg taataatgag tcctgcccaa agtgcggggc tgaactggtt attcgatccg
    23161 ggaaacacgg tccgtttctt ggatgctcac agtatccggc gtgtgactac gtccgtcctc
    23221 tgaaatcttc agcggatgga catatcgtca aagttctgga ggggcaggtt tgccctgcat
    23281 gtggcgcaaa tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact
    23341 accctgaatg cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacatgtc
    23401 cccaatgccg gacgggccat ctggtccagc gccgctcccg ttatggcaaa acatttcact
    23461 cctgtgatcg ctacccggag tgtcaatttg ccattaactt caaacccata gctggagaat
    23521 gccctgagtg tcattatccg ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact
    23581 tttgtgccag taaacaatgt ggaaagccgg tttcggcgga ataataacgt gaataataac
    23641 ctgcaaggag acgctatcgc agctgcgata gatgttctca atgaagaacg tgtcatcgcc
    23701 tatccaacgg aagccgtttt cggtgttggg tgcgatcctg atagcgaaac agcagtgatg
    23761 cgactgttgg agttaaaaca gcgtccggtt gataaggggc tgattttaat cgcagcaaat
    23821 tacgagcagc ttaaacccta tattgatgac accatgttga ctgacgcgca gcgtgaaacc
    23881 attttttccc gctggccagg tccagtcacc tttgtctttc ccgcgcctgc gacaacaccg
    23941 cgctggttga cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg
    24001 gttgctttgt gccaagctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga
    24061 ttgccacctt gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc gttcccggtt
    24121 gtgcctggtg aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt
    24181 gaactgtttc gacaggggta acataatgga aacctatgct gtttttggta atccgatagc
    24241 ccacagcaaa tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc
    24301 ctatgggcgc gtgttggcac ccatcaatga tttcgtcaac acactgaacg ctttctttag
    24361 tgctggtggt aaaggtgcga atgtgacggt gccttttaaa gaagaggctt ttgccagagc
    24421 ggatgagctt actgaacggg cagcgttggc tggagcagtt aataccctca agcgtttaga
    24481 agatggacgc ctgctggggg acaataccga tggtataggc ttgttaagcg atctggaacg
    24541 tctgtctttt atccgtcctg gtttacgtat tctgctcatc ggcgctggtg gagcatctcg
    24601 cggcgtacta ctgccacttc tttccctgga ctgtgcggtg acaatcacta atcgaacggt
    24661 atcccgcgcg gaagagttgg ctaaattgtt tgcgcacact ggcagtattc agacgttggg
    24721 tatggacgaa ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat
    24781 cagtggtgat attccggcga tcccgtcatc gctcattcat ccaggcattt attgctatga
    24841 catgttctat cagaaaggaa aaactccttt tttggcatgg tgtgagcagc gaggctcaaa
    24901 gcgtactgct gatggtttag gaatgctggt ggcacaggcg gctcatgcct tccttctttg
    24961 gcatggcgtt ctgcctgatg tagaaccagt tataaagcaa ttgcaggagg aattgtccgc
    25021 gtgaatcagg ccatccagtt tccggacaga gaagagtggg tcgagaataa aaaatgtgta
    25081 tgttttcccg ctctcgtgaa tggcatgcaa ctgacatgcg cgatctctgg cgatagcctg
    25141 gcgtatcgct ttactggaga tacgccagag cagtggttag cgagttttcg tcagcatcgc
    25201 tgggacctgg aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggc
    25261 tgggtctggt taccctgatc cagatattcg tccttccatt tcacgtaatt attcgcggaa
    25321 tagcgtaacc cagccttctc ttcatcactt aacgggcgga tctgtttgac gggactaccg
    25381 agatacagat atccgctctc cagccgttta ttttgtggga ccagactacc cgcaccaatc
    25441 atcacatcat cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact
    25501 cgattgccaa tggtgcagcc gtggagcatc accttgtgac caacagtgac atcttcgcca
    25561 atggttaatg ggttgccatc tgggttgtac gaggatttat gagtgacatg caacatactg
    25621 ccatcctgga tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca
    25681 acgagcggcc agatccccac atcatcagcc agacgaacgt caccaatcac gacactgcta
    25741 tcgtcgatca ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg gcgtaaaaca
    25801 tcagacatac ttacctcagc aataaatgat ttactaatga ctttgggggc attattggcc
    25861 ttgtgcaagt cttttagtat gcaaaaaagc accgttttgt gtgcgattgc agcaaaaagg
    25921 gtgaaaaaac aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa aattgggatc
    25981 cctataatgc gcctccgttg agacgacaac gtgaaacact tcacaggatg gtcggaacaa
//
LOCUS       DAPGRN010000066        25506 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPGRN010000066 DAPGRN010000000
VERSION     DAPGRN010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.066"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(302..2119)
                     /gene="malZ"
                     /locus_tag="RHW33_002870"
     CDS             complement(302..2119)
                     /gene="malZ"
                     /locus_tag="RHW33_002870"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV1646354.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVVQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(2275..3648)
                     /gene="proY"
                     /locus_tag="RHW33_002871"
     CDS             complement(2275..3648)
                     /gene="proY"
                     /locus_tag="RHW33_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV1646355.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(3724..5043)
                     /gene="brnQ"
                     /locus_tag="RHW33_002872"
     CDS             complement(3724..5043)
                     /gene="brnQ"
                     /locus_tag="RHW33_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV1646356.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(5450..6745)
                     /gene="phoR"
                     /locus_tag="RHW33_002873"
     CDS             complement(5450..6745)
                     /gene="phoR"
                     /locus_tag="RHW33_002873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV1646357.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(6803..7492)
                     /gene="phoB"
                     /locus_tag="RHW33_002874"
     CDS             complement(6803..7492)
                     /gene="phoB"
                     /locus_tag="RHW33_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV1646358.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            7682..8884
                     /gene="sbcD"
                     /locus_tag="RHW33_002875"
     CDS             7682..8884
                     /gene="sbcD"
                     /locus_tag="RHW33_002875"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV1646359.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITEQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8881..12024
                     /gene="sbcC"
                     /locus_tag="RHW33_002876"
     CDS             8881..12024
                     /gene="sbcC"
                     /locus_tag="RHW33_002876"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV1646360.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCVDGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLKASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQTLQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMTLRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRTLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQKIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQHRQN
                     ELTALQNRIQQLTPILETLPQSDELPHCEETVVLENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALVDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            12150..13334
                     /gene="araJ"
                     /locus_tag="RHW33_002877"
     CDS             12150..13334
                     /gene="araJ"
                     /locus_tag="RHW33_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV1646361.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(13468..14376)
                     /gene="mak"
                     /locus_tag="RHW33_002878"
     CDS             complement(13468..14376)
                     /gene="mak"
                     /locus_tag="RHW33_002878"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV1646362.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGLRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEEND
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            14501..15412
                     /gene="rdgC"
                     /locus_tag="RHW33_002879"
     CDS             14501..15412
                     /gene="rdgC"
                     /locus_tag="RHW33_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV1646363.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(15490..15945)
                     /locus_tag="RHW33_002880"
     CDS             complement(15490..15945)
                     /locus_tag="RHW33_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646364.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(15942..18221)
                     /locus_tag="RHW33_002881"
     CDS             complement(15942..18221)
                     /locus_tag="RHW33_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HDV1646365.1"
                     /translation="MNRINILVICMVVFFMTGNACATEWISSEELITSDFHLMTADER
                     NVVKAATDDSMEAAYMLKDNIRWYYHNGDLSLPANFSNQNKLVVNGNLTISGDYDDYL
                     SGNGHLIVLGNVIVDNFINHDFAYVKGQMTAKGLVYADYNDHNFEVMKGISARGIIVS
                     DKATQFEVIKAEFYINEDGSGEGYNWDENIQKAYSLVTADLYDHTEIETDNISNAYPD
                     YDSVADNIVQGLPLFRDKAAPEINEKLKWIETGKLDNFPANKIKHQDPLVARFLTHTE
                     SLSPAVMLQLLQHPDDQTRESMAQSWPAQQMHLLTDELIKDEAVARGLVKNSNISADV
                     NTKLMSVPVESVQLEQARQDNLSPDIVASLSHSPFLSVRKTLLSHYDYAWLVPTAVAD
                     ELINNEDPELRERITGADLTAQQAVMLSKDKSLKVREALARTLTELKITQLSATLRTE
                     DIERIAEQMYLDNKENKNIVKALLIALPEMRQLSLAKEDVHNLREGARYLTSREVISY
                     LLTQHDVPTVWDELARDKLLPLEYKKQLWQRTLNLMMSKRQEDQEQAYEVQLALIDNG
                     VVDEEMLNNAIDLLVDLPAEYRYRMRNQLFDNKELPSGIINKLDQQYRFNSDWALAVV
                     SMKNSTRRQSERGLHRWNREDSDIFAKLATIKDKSDDEWWRALLQSRNDHLRQTALRN
                     AHTPASLLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSLLRDLVK
                     TGATRKIRSEARHRLEEKQ"
     gene            complement(18365..18649)
                     /gene="ppnP"
                     /locus_tag="RHW33_002882"
     CDS             complement(18365..18649)
                     /gene="ppnP"
                     /locus_tag="RHW33_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV1646366.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(18721..19398)
                     /locus_tag="RHW33_002883"
     CDS             complement(18721..19398)
                     /locus_tag="RHW33_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV1646367.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(19656..19847)
                     /gene="yaiA"
                     /locus_tag="RHW33_002884"
     CDS             complement(19656..19847)
                     /gene="yaiA"
                     /locus_tag="RHW33_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV1646368.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(19897..20421)
                     /gene="aroL"
                     /locus_tag="RHW33_002885"
     CDS             complement(19897..20421)
                     /gene="aroL"
                     /locus_tag="RHW33_002885"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV1646369.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(20604..21062)
                     /locus_tag="RHW33_002886"
     CDS             complement(20604..21062)
                     /locus_tag="RHW33_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV1646370.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            21182..21991
                     /gene="proC"
                     /locus_tag="RHW33_002887"
     CDS             21182..21991
                     /gene="proC"
                     /locus_tag="RHW33_002887"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV1646371.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(22008..23123)
                     /gene="adrA"
                     /locus_tag="RHW33_002888"
                     /gene_synonym="dgcC"
     CDS             complement(22008..23123)
                     /gene="adrA"
                     /locus_tag="RHW33_002888"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV1646372.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVMLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(23225..23545)
                     /gene="psiF"
                     /locus_tag="RHW33_002889"
     CDS             complement(23225..23545)
                     /gene="psiF"
                     /locus_tag="RHW33_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV1646373.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATVQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(23664..25079)
                     /gene="phoA"
                     /locus_tag="RHW33_002890"
     CDS             complement(23664..25079)
                     /gene="phoA"
                     /locus_tag="RHW33_002890"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV1646374.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTADQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(25180..25440)
                     /gene="iraP"
                     /locus_tag="RHW33_002891"
     CDS             complement(25180..25440)
                     /gene="iraP"
                     /locus_tag="RHW33_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV1646375.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         6447 a         6747 c         6225 g         6087 t
ORIGIN      
        1 atgggggctt tttttaaatt gttcttctgt tttagaaaag acatcatcct taaaaggtag
       61 caacttagaa ccagtaataa acggttttaa taaattcgaa tttgaaattg tcatatcttt
      121 gctccactac agggttatcc tggagcatgt tgtacataac atcatgggca aagaatacca
      181 aaaatcgctc atatcgatcg ggacaaatat ttaacaaatt cagccctgac attgagcagc
      241 ttttttgcgc tgcgtagtgt cagggctggt gactaacgac gtaacaaata aaacagatgc
      301 tttagttcat ccacaccgta gccgaaatgg caggcaaagc cagaatcccg tctgtcagtt
      361 gcccatggcc ttctttgcgt tgccattgca cgacattaag aaacggtgac gcgggtagca
      421 ccacttcaca ggcttcgcca cggttaattg ccaccagtac acgctgctga ttcagcacgc
      481 ggacaaatac caccacgtta tcttccgcat acagcacctg acagccgcca cgacgcagcg
      541 cctgactttt cttacgcagc acaatcattc gctggtacag cgcgaataac gccgtatcct
      601 gcttttccac ctgccaggga aacggtttac ggcaaaacgg atcgttttta ccatccagtc
      661 ccacttcatc accgtaataa atgcacggta caccaggcca ggtgaacagc cagaccaccg
      721 ccaggggcag gcgcgcaaca tcccgaccga gcagcgtttt aaatcgcgca gtatcgtggc
      781 tgtcgagctg attaaacata cgtaattgct gttgatgaga aagccctgcg cggtaattat
      841 ccatccaggc catacaggtt tgagcgtcaa tttgctgcgg atcgtaagag atgtcggtat
      901 tggcaagaaa tccccacaac gggaatgtga agccgcgata gttcatggcg gcatcttcca
      961 catcggcctg taaccattgc cgtgcatcgc caaaatgctc tccaactata tacgcttctg
     1021 gctgggtttc tttcgccgct tcggtgatcc cggcaacgtg ctgcaaatta ttgcgcgccc
     1081 cacccgcctc ccccagcata tgcaccacat ccagccgcca gccgtccata ttccacggcg
     1141 ctttcagcca gtggcggaca atactgtctt ccccgcgata aatttcattc accagacttt
     1201 ccgactgata atccagcttc ggcaagctgg catagccaag ccagtcgagc gccgtgccat
     1261 catcactaaa cgagtaccag tcgcgccagg gcgattcggg gttgtgacaa gctccccccg
     1321 tgccacgatt gtgcctgtca aaccaggcat gggaatcgcc actgtggtta aacacgccgt
     1381 ccagcaccag ccgcattccc agctgctgcg tattgtgtcg caaacgcagc aacgccccat
     1441 caccgccaaa ctgcggatcg acatggcgat aatcctcggt atcgtattta tgtacgctgg
     1501 gagctttaaa caccggattg agatacaacg ccgtcacgcc aagttttttc agatacggca
     1561 gtttttcgct tatcccgtcc agatcgccgc catagaacgt tgatccgccc gcctgcgccg
     1621 tgaccggttc atcccagtca cgcaatatga tctcttgtcc ggctgcgtga tggtaataga
     1681 catgatcctg ttcagcttca cgaggaaggc tacgggcgaa acgatcaggg aagatctgat
     1741 aaaaaatctg atccgcagcc cattgtgggc cgatatccgg tacatcgatg gcaaactgct
     1801 ccagtcgtgc tggcggcatt cggctgaagc cctgcggtgt aaaccaacgc tggcgatcgt
     1861 gccatagcaa tttgaaacta taacgccgcc gaggttgtcc gctggagaga tcgatcgccg
     1921 cccgccatgc ggtgacacca ggctgcggct gactgcgctg cttatgcatc ggtactgaca
     1981 tttcttcgtt atcgtgttct gtacgcagca taatgcgctg cggtgggtct tcgcccgtca
     2041 gccacagtgt aatgagcagt tgatctttgc tttgtttaac aaatgggggc accggcaggt
     2101 gccatgcatt taacatcata attcccctgt gatgaaaatc tgatcacctt gccatagcct
     2161 aatgcaggat cactcatcat ctgaaggata tttgggggag gaggacgggg gcggataact
     2221 ttcggaatag ccggatcagg atttcctcct gaaccggctt acagaacgca aggattactg
     2281 gttttcagcc agctgacgat cgtggcggcg tttaaacatc cagccaatca acagcacaac
     2341 aatccacgcg aaaccgacat acagcgagat acgcgtatcc gggtgataac caatcaaccc
     2401 gataataaag agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac ccggcacttt
     2461 aaatttcagc gccttaactt cttctggcgg caaacggcga cggaaggcaa tttgcgacag
     2521 caggatcata atccacaccc ataccgtggc gaaggttgcc agcgaagcga tcaccaggaa
     2581 gacgttttcc ggcatgatgt agttcagata caccgcaaac aatagcgcgg tagtcatcac
     2641 cagcaccgta acccacggaa taccgcgacg cgacgttttg ctgaaaattt tcggtgcgct
     2701 gccctgctct gccataccgt ggagcatacg gcctacgcca aatacgtcac tgttaattgc
     2761 cgacagcgaa gcagtcagca caacaaagtt aagaatgctg gcggcaaagg taatgcccat
     2821 atgctggaac gtcagcacga acgggctacc ggcagtgcca acctgattcc acgggtagat
     2881 agacataatg acgaacagcg taccgacgta gaacaccaga atacgcatcg gcacggagtt
     2941 aatcgcacgc ggtatcgatt tctcaggatc tttcgcttca ccggcggtaa tcccgataat
     3001 ttcgatccca ccgtaagcaa acatcaccat ttgcaacgac atcaccatcc caagccagcc
     3061 gttactgaag aagccgccgt tgctccacag gttatgaata ccggtcggtt gcccgccgtt
     3121 gccaatcccc cagatgatga tgccgaaacc ggcgacaatc atgatgatga tagtggcgac
     3181 tttaaagaac gagaaccaga attccagctc accgaatacc ttcacgctca tcaggtttac
     3241 ggcgcaaatg atcagcacca cgctcagtac ccaaatccag tgcggcaccg tcgggaacca
     3301 gacacccata tagataccaa aagcggtcac atcggcgatg gcgacaataa ggatttcaaa
     3361 gcagtaggtc caaccggtaa tgtaacctgc cagcgggccg aggttttcct gcgcataacg
     3421 cgagaaagag ctggcggccg ggttatgtac cgacatttcc cccagcgcac gcataatgat
     3481 atacgccgcg ataccaccga taatataggc caacaacacg ctcggaccgg ccattttgat
     3541 ggcgtctgcc gaaccgtaaa acagcccggt gccaattgct gaacccagtg ccataaagcg
     3601 tatgtggcgg gtacttagcc cacgctttag cttgttctta ctttccatca attcctaacc
     3661 catcaacaca ataaaaaacc acggggcatc gcagccccgt ggttaaaaca aatgttcagt
     3721 gatttagtga gcgctggagg tcacctgacg acctgccgcg cgatcccaga taatggccag
     3781 aaccaccatc accactgttg gcattaacca cgccagacct tgttcggcca gcggtaaacg
     3841 ctgcgcccag gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc
     3901 aaaaagcagg ctgataaaca tcggcggagc aatcacgcgg gacgaattat gccaccatga
     3961 gcgtgtaaaa cttaatacaa ccagtgcgat acacggcgga taaatggcgg tcagcaccgg
     4021 tacggagatc tgaatcagtt ggctcaagcc gaggttagaa accaccatcg agaagccgcc
     4081 gaggataaac accagcgtac gataagagag cggtacgtac tgggcaaaga attctgcaca
     4141 agcacaggtc aggccaactg ccgttaccag gcaggcgatg aagattaacg ccgccagcag
     4201 gaagctaccg ccgccgccaa aggtgtgctg aacgtaagca tgcagaatag ccgcgccgtt
     4261 tgcagactga tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg ccaggtacag
     4321 cagagtcaga ccaacacccg ccatcaggcc agcccagacg gtataacggg tcagcagacg
     4381 cgcttcggta acgccacgag aacgcgccgc gttaacaata acgataccaa acaccattgc
     4441 gcccagcgta tccatggtca gatagccgtt aacaaagccg ttagaaaacg cagcgttttg
     4501 ataagcctca gtcgccgtgc tgatagaacc cgccggccag acaatcgcgg caacagacag
     4561 gatgaccagc gcgataattt tcagcggcgc aaggaagttg cccacggtat ccagcagctt
     4621 gcccggatag agcgaaacca gaataacgat agcgaaatag accaggctgt agataaacag
     4681 cggcagcgcg gaatcacccg tcagcggcgc aatccccact tcaaaggaaa cggtagctgt
     4741 acgcggcgta gcgaaaagcg gccccaccgc caggtaacaa accgttgcca gcagtacgcc
     4801 agcgacttta ccgattggcg tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc
     4861 cactaccgtt aataccggca ggccaacggc agtaatgagg aagccgaatg ccgcagtcca
     4921 gacgtgttcg cctgcctgca agccgaccat tggagggaaa ataatgttac ctgcgccgac
     4981 gaacaacgca aatgtcataa agcccagagc gatgatatcg cgcgatctta attgatgggt
     5041 cataaatcat actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg
     5101 gggatgatac agcggtatat ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg
     5161 cgaagaatag tttttcgatt taccgcgtta atgcagtcag tatgacagca cctgaagcgc
     5221 aatttaaacg cttataacgt tttaaagcaa ggagggatct aaaaaccaga ggtatatagc
     5281 gataagaaat caccaaccag tataaaacac tatttccgtg aaaaagacat ggctaatcat
     5341 gcgaacaaaa aatcaccaac cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa
     5401 ataagccagc attggctggc ttatggcaat aaaagatgac aaaggcggat taatcgctgt
     5461 ttttggcaat taaacgttcc gggataacaa agctgaaacg tgttcctttt cctactgtac
     5521 tctcaatatt caggcgactt tcgtgatgat tcaccgcatg tttcacgatc gctaacccta
     5581 atccgctacc gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca
     5641 ggcgcggaat atgctccggt gcaatgcccg gtccgttatc ttcaacgcta aattcggcac
     5701 cgtgcggcac tcgctgccag cgtacggtga tatgcgtgcc ttctggagta tgattcacgg
     5761 cgttatagac caggttcgaa atcgcactgc gtagctgatc ttcgttgcca gacaccttga
     5821 ggccgttatc tatctcaaag gtaaatgtct gttttttctg actcagagtc tgagcctcac
     5881 gctcaacaac gcgcagcatc atcggcacat caaccttttc attgagcaaa tgcgtcggtg
     5941 cggcttctat tttcgacagc gtcagcaatt gcttcaccaa cccttccatc cgctgggtct
     6001 gctcgcgcat ggtgtgcaac gctttttcac gtaccgcgcc ttccagcggc tgctcatcca
     6061 tcatctccag gtaaccctgt aacacggtca atggcgtgcg taactcatgg cttacgttgg
     6121 cgaaaaagtt gcgccgcgcc ccttccagtt gatgcatttg cgtgacatca cgcgccacca
     6181 tcagcaactg tttgtgggta taaggcatga cgcgaatttc cagatgccgc ccggtgttga
     6241 gcaccagatt gagcgggcga gaaaaatcac gcgttttcag atattgcgta aactccgggt
     6301 aacgcagcag gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa ccaagaatct
     6361 gttgcgccag accgttacac cagaaaatac cgccctcttc cgtggtcagc accactgcgt
     6421 cgggcagcga ctccgcgccg ctacgaaagc gtttaatcag attgcccagt tcacggcggc
     6481 gttttttatt tcgcagctgc atctggtgta agccgtatag tagcggttcc cagctaccac
     6541 gccccggtgg cggggtcata ctacgatcca cccacagcca ccatgaaagg cgcaataaat
     6601 tccagaaatg ccagataagc agtcccgtta ccgatgccag caaaaaccag ggtaggtaac
     6661 caaaaaatgc acccaggatg aaagccggga ggcagcaaag tagcagctcc agcaccagcc
     6721 ttttccacga cagccgttcc agcacgtaag atactccagt taagaaatca taagccctgc
     6781 tctgcgtccg atgagcaagg cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca
     6841 cggtctgcac catgcggtca tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt
     6901 ggacatcgac cgtgcggtct tccacataca cgttagttcc ccagacgtgg tttaacagct
     6961 gctcgcggct gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg
     7021 tcggccccat ctccagcggc tcttcgcctg ccatcacccg gtgagatgtc gggtcgagac
     7081 ttaatccctg catctcaatc acctcttcca ccgccattgg cgaaatacgg cgcattaccg
     7141 ctttgattcg cgccaccagc tccttcggcg aaaacggctt ggtgatatag tcatccgcgc
     7201 cggtttcaag gccgcgcacg cgatcttctt cttcccctct ggcggtcaac atcaccactg
     7261 gaatatcccg ggtcatcgac tcgcgcttta ggtgtttgat gaactggata ccggagccgc
     7321 caggtaacat ccagtcaaga agaattaaat ccggccaggg ttcattcagt tgattcacag
     7381 cactgtcata atcttccgct tcgaccggct gaaagccatt ttgttcgagc acgaagcaga
     7441 ccatttcgcg aattggagct tcatcttcta cgaccagaat acgtctcgcc atgatttgcc
     7501 ctgttgtaat aaataggttg cgatcattaa tgcgacgtca ttatgcgtca gatttatgac
     7561 agatttatga aaagctcgtc gcacatatct tcaggttatt gatttccgtg gcgcagaaaa
     7621 aagcaaatgg cacatctgtt tgggtataat cgcgcccatg ctttttcgcc aggtaaccgt
     7681 tatgcgcatc cttcacacct cagactggca tctcggccag aacttctata gtaaaagccg
     7741 cgaagctgaa catcaggctt ttcttgactg gctgctggag acagcacaaa cccatcaggt
     7801 ggatgcgatt attgttgccg gtgatgtttt cgataccggc tcgccgccca gttacgcccg
     7861 cacgttatac aaccgttttg ttgtcaattt acagcaaact ggctgtcatc tggtggtact
     7921 ggcaggaaac catgactcgg tcgccacgct gaatgaatcg cgcgatatca tggcgttcct
     7981 caatacgacc gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg
     8041 gacgccaggc gcagtgctgt gccccattcc gtttttacgt ccacgtgaca ttattaccag
     8101 ccaggcgggg cttaacggta ttgaaaaaca gcagcattta ctggcagcga ttaccgatta
     8161 ttaccaacaa cactatgccg atgcctgcaa actgcgcggc gatcagcctc tgcccatcat
     8221 cgccacggga catttaacga ccgtgggtgc cagtaaaagt gacgccgtgc gtgacattta
     8281 tattggcacg ctggacgcgt ttccggcaca aaactttcca ccagccgact acatcgcgct
     8341 cgggcatatt caccgcgcac agattattgg cggcatggaa catgttcgct attgcggctc
     8401 ccccattcca ctgagttttg atgaatgcgg aaagagtaaa tatgtccatc tggtgacatt
     8461 ttcaaacggc aaattagaga gcgtggaaaa cctgaacgta ccggtaacgc aacccatggc
     8521 ggtgctgaaa ggcgatctgg cgtcgattac cgaacagctg gaacagtggc gcgatgtatc
     8581 gcaggagcca cctgtctggc tggatatcga aatcactact gatgagtatc tgcatgatat
     8641 tcagcgcaaa atccaggcat taaccgaatc attgcctgtc gaagtattgc tggtacgtcg
     8701 gagtcgtgaa cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact
     8761 cagcgtcgaa gaggtgttca atcgccgtct ggcactggaa gaactggatg aatcgcagca
     8821 gcaacgtctg cagcatcttt tcaccacgac gttgcatacc ctcgccggag aacacgaagc
     8881 atgaaaattc tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga atggaagatt
     8941 gatttcaccc gcgagccgtt cgccagcaac gggctgtttg ccattactgg cccaacaggt
     9001 gcggggaaaa ccaccctgct ggacgctatt tgtctggcgc tgtatcacga aactccgcgt
     9061 ctctctaacg tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc cgaatgtctg
     9121 gcggaagtgg agtttgaagt gaaaggtgaa gcgtatcgcg cgttctggag ccagaatcgg
     9181 gcgcgtaacc aacccgacgg taatttgcag gtgccacgcg tagagctggc gcgctgcgtc
     9241 gacggcaaaa ttctcgccga caaagtgaaa gataagctgg aactgacagc gacgttaacc
     9301 gggctggatt acgggcgctt cacccgttcg atgctgcttt cgcaggggca atttgctgcc
     9361 ttcctgaatg ccaaacccaa agaacgcgcg gaattgctcg aggagttaac cggcactgaa
     9421 atctacgggc aaatctcggc gatggttttt gagcagcaca aatcggcccg cactgagctg
     9481 gagaagctgc aagcgcaggc cagcggcgtc gcgttgctca cgccggaaca agtgcaatcg
     9541 ctgaaagcga gtttgcaggt acttactgac gaagaaaaac agttacttac cgcgcagcag
     9601 caagaacaac aatcactaaa ctggttaacg cgtctggacg aattgcagca agaagccagc
     9661 cgccgtcagc agaccttgca acaggcgtta gccgaagaag aaaaagcgca acctcaactg
     9721 gcggcgctca gtctggcaca accggcacga aatcttcgtc cgcactggga acgcatcgca
     9781 gaacacagcg cggcgctggc gcatactcgc cagcagattg aagaagtaaa tactcgctta
     9841 cagagcacaa tgacgcttcg cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa
     9901 ttacagcagc agcaacaaag cctgaatacc tggttacagg aacacgaccg cttccgtcag
     9961 tggaacaacg aactggcggg ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag
    10021 catctgcggc aatggcagca acagttaacc catgctgagc aaaaacttaa tacgcttgcg
    10081 gcgatcacgt tgacgttaac cgccgatgaa gttgctagcg ccctggcgca acatgccgag
    10141 caacgcacgc tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc ccaacaaaaa
    10201 cgtctggcgc agttaatggt cactatccag aatgtcactc tggaacagac gcaacgtaat
    10261 gccgcactta acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg
    10321 aaaaccattt gcgagcagga agcgcgcatc aaaacgctgg aagcccagcg cgcgcagtta
    10381 caggcgggtc agccttgtcc actttgtggt tccaccagcc atccagcggt cgaggcgtat
    10441 caggcgctgg aaccgggcgt taatcaggcc cggctattaa cgctggaaaa agaagtgaaa
    10501 aagctcggcg aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac gaagcagctt
    10561 cagcgcgatg aaaacgaagc gcaaagcctc cgacaagatg agcaagcact tactcaacaa
    10621 tggcaagccg tcacgaccag cctcaatatc accttgcagc cacaggacga tattcaaccg
    10681 tggctggatg cacaagatga gcacgaacgc cagctgcggt tactcagcca acggcatgaa
    10741 ttacaagggc agattgccgc gcataatcag caaattatcc agtatcaaca gaaaattgaa
    10801 caacgccagc agcaactctt aacggcattg gcgggttatg cactgacatt gccacaggaa
    10861 gatgaagaag agagctggtt ggcgacacgt caacaagaag cgcagagctg gcagcatcgc
    10921 cagaacgaat taactgcgct gcaaaaccgt attcagcagc tgacgccgat tctggaaacg
    10981 ttgccgcaaa gtgatgaact cccgcactgc gaagaaactg tggtgctgga aaactggcgg
    11041 caggttcatg aacaatgtct cgcattacac agccagcagc agacgttaca gcaacaggac
    11101 gttctggcgg cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc gctacaggcc
    11161 agcgtctttg acgatcagca ggcgttcctt gcggcgctag tggatgaaca aacactaacg
    11221 cagctggaac agctcaagca gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc
    11281 actcagacag cagaaacgct ggcacagcat cagcaacacc gacctgacgg gttggctctc
    11341 actgtgacgg tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa
    11401 aacaccacga gtcaaggcga gattcgccag cagctgaagc aggatgcaga taaccgtcag
    11461 caacaacaaa ccttaatgca gcaaattgct caaatgacgc agcaggttga ggactgggga
    11521 tatctgaatt cgctaatagg ttccaaagag ggcgataaat tccgcaagtt tgcccagggg
    11581 ctgacgctgg ataatttagt ccatctcgct aatcagcaac ttacccggct gcacgggcgc
    11641 tatctgttac agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga tacctggcag
    11701 gcagatgcgg tacgcgatac ccgtaccctt tccggcggcg aaagtttcct cgttagtctg
    11761 gcgctggcgc tggcgctttc ggatctggtc agccataaaa cacgtattga ctcgctgttc
    11821 cttgatgaag gttttggcac gctggatagc gaaacgctgg ataccgccct tgatgcgctg
    11881 gatgccctga acgccagtgg caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa
    11941 gagcgtattc cggtgcagat caaagtgaaa aagatcaacg gactgggcta cagcaaactg
    12001 gaaagtacgt ttgcagtgaa ataactattc agcaggataa tgaatacaga ggggcgaatt
    12061 atctcttggc cttgctggtc gttatcctgc aagctatcac tttattggct acagtgattg
    12121 gtagccgttc tggtggttgt gatggtggta tgaaaaaagt cattttatct ttggctctgg
    12181 gcacgtttgg tttggggatg gccgaatttg gcattatggg cgtgctcacg gagctggcgc
    12241 ataacgtagg aatttcgatt cctgctgccg ggcatatgat ctcgtattat gcgctggggg
    12301 tggtggtcgg tgcgccaata atcgcactct tttccagccg ctactcactc aaacatatct
    12361 tgttgtttct ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt
    12421 acctgatgct tgccattggt cgcctggttt ccggctttcc gcatggcgca ttttttggcg
    12481 tcggagcgat cgtgttatca aaaattatca aacccggaaa agtcaccgcc gccgtggcgg
    12541 ggatggtttc cgggatgaca gtcgccaatt tgctgggcat tccgctggga acgtatttaa
    12601 gtcaggaatt tagctggcgt tacacctttt tattgatcgc tgtttttaat attgcggtga
    12661 tggcatcggt ctatttttgg gtgccggata ttcgcgacga ggcgaaagga aagctgcgcg
    12721 aacaatttca ctttttgcgc agcccggccc cgtggttaat tttcgccgcc accatgtttg
    12781 gcaacgcagg tgtgtttgcc tggttcagct acgtaaagcc atacatgatg tttatttccg
    12841 gtttttcgga aacggcgatg acctttatta tgatgttagt tgggctaggg atggtgctgg
    12901 gaaatatgct aagtggcagg atttcaggac gttattcacc actgcgcatt gcagcagtga
    12961 ctgactttat aattgtactg gcactgctga tgctcttttt ctgcggcggc atgaaaacaa
    13021 cgtcgcttat ttttgctttt atttgttgcg cgggattatt tgccctttca gcacctctgc
    13081 aaatattgtt actgcaaaat gccaaaggcg gagagttatt aggtgccgca ggtgggcaaa
    13141 tagcgtttaa cctcggtagc gccgtcggcg catattgcgg aggtatgatg ctgacgctgg
    13201 ggctggcata taattacgtg gcgctgcctg ccgccctgct ttcgtttgct gcgatgtcat
    13261 cgttgctgct gtatggtcgc tataagcgcc agcaagcggc ggatagtccg gtgctggcga
    13321 aaccactggg gtagattgca gtcacggagc acgttgctta ttgccggatg cggcgtgaat
    13381 gccttacgcg gttctggcac attttgtagg cctgataaga cgcggcaagc gtcgcatcag
    13441 gcatgatgtg ccaattgcct gggtttttta ctcttgtggc cataaccacg cagcgccgcg
    13501 tacgccgctg gaatcaccat gcttcgcctt acgcaccggc gtttcacatt cgccgccgaa
    13561 gacaaattgt ttaatcaact gcggaaccgt ttgatataaa cggtctacat tgctcatccc
    13621 gccccccagg acaatcacat ccggatcgag aatattcacg acatgtgcca gcgattttgc
    13681 cagccgcagc tcgtagcgac gcaatgccag ttccgctacc ggatcgtttt cttcaaccag
    13741 gcggataatt tcactgcctt tcagcgcatg tccgctcaaa cgacgataat ccgtcgcgaa
    13801 tcccgtgccc gaaataaagg tttcaataca accttgttta ccgcaataac aagggacttc
    13861 ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc ggattgtgtc cccactcacc
    13921 tgccgtgcca ttgccgccga tatgcgcccg cccattgaat gccacgcccg cgccgcatcc
    13981 cgtgccgata atcacggcaa ataccgtctg cgctcccgct gccgcgccat ctactgcttc
    14041 tgaaaccgcc agacagttgg cgtcatttgc cagccgcact tcccgctgca acctcgcgct
    14101 taagtcttta tcgaatggct gaccgttgag ccaggttgaa ttggcattct tcaccacacc
    14161 ggtgtaaggc gaaattgagc caggaatgcc catacctacc gttccgcgca gccccgtcgc
    14221 ctgctccgcc atatcaacca acgtggcgat cgtttcaata gtctgccggt aatcatcacg
    14281 cggcgtgggc agacgatggc ggtacaactg ctcccctgca tcgcccagtg caatcacttc
    14341 agttttggtg ccgcctaaat cgatacctat acgcacggta ctctccttat ttttttcaat
    14401 atcaatagcg tagagacgga caaccggatt ggcaatgcaa ggccgccgac aattcgttat
    14461 catgcccgct aaatttaacg acaaggccgt ggaaattatc atgctgtggt tcaaaaattt
    14521 aatggtttac cgtcttagcc gcgagatttc gctgcgtgca gaagagatgg aaaaacagct
    14581 agcctcgatg gcatttaccc catgcggcag ccaggacatg gcgaagatgg gctgggttcc
    14641 tccgatggga tcgcacagcg atgcgttaac gcacgttgcc aatggtcaaa ttgttatctg
    14701 cgcacgcaaa gaagaaaaaa tcctcccgtc tccggtgatt aaacaggcgc tggaagcgaa
    14761 aatcgccaaa ctggaagcgg aacaggcgcg taagctgaag aaaaccgaaa aagattcgct
    14821 gaaagacgaa gtgctgcact ctctgttgcc gcgtgctttc agccgtttta gccagacaat
    14881 gatgtggatc gacacggtta acggtttgat tatggtggac tgcgccagtg ccaaaaaagc
    14941 ggaagatacg ctggcactgc tgcgtaaaag cctggggtcg ttaccggttg taccattgag
    15001 catggaaaac ccgattgaac tgacgctgac cgaatgggta cgttccggta gtgcggcaca
    15061 gggcttccag ctgcttgatg aagccgagct gaaatcgttg ctggaagatg gcggagtgat
    15121 ccgcgcgaag aaacaagatc tgaccagcga agagatcacc aatcacattg aagccggaaa
    15181 agtggtgact aaactggcgc tcgactggca gcagcgcatt cagtttgtga tgtgcgacga
    15241 tggttcgctc aagcgtctga agttctgcga cgagctgcgc gatcaaaacg aagatatcga
    15301 ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc ctgatgactg gtgaactggc
    15361 agcgttaatt caaaacctga ttgaaggatt aggtggcgaa gcacaacgtt aattgctgat
    15421 tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc cggcgtacga agccacgcca
    15481 ggcatataat tattcgctac ggcgggcaat aatttttagc gcagcaatat tatgcgtttt
    15541 acgccgtaac tgatctaatc gcctcatccc tattcgcctg tcagcagcac gcagaatatt
    15601 cactaacccc catttatcgc tggtaaattg ttttgttaac aactccgctt ttggggtgtc
    15661 aatatattca cgcaataaat cagaaattga acaaatatcc gtcaggtaag gataccagca
    15721 agtttcaccg tgaaaattac gcactccacc gttacctgca ataaagtctt tcgggtaatc
    15781 ccagatcacg tttttatcca acgtgatcca ataacgcggt aaatcggtgc ttccaccatt
    15841 ttggctgcgc atgggataac gggtacagtg aatttgcagg ttgatcgttg gcgtcagtaa
    15901 gtcatagatc tcccgctgta acttgctcca tggacgttgt gtcattgttt ttcctcaagc
    15961 cggtgacgcg cttcactgcg aattttccgc gttgccccgg ttttcactaa atcacgtaac
    16021 agcgaaagat cgggttgttc gacaaataag agtaatgatg gatcctcttt taaccacgcc
    16081 gttttcacat cggcagccag ctgtggatta ttaatagcca gcgaccgatc ctgaggctca
    16141 gtcaatgtcg ttaacaacga cgccggggta tgtgcgttgc gtaatgccgt ttggcgcaaa
    16201 tgatcattgc gactttgcag caatgcacgc caccattcat catctgattt atcttttatc
    16261 gtcgccagtt tcgcaaaaat atcgctatcc tcacggttcc agcggtgtaa tcctctttca
    16321 ctttgcctac gggtgctatt tttcatactg acaacagcca gcgcccagtc agagttgaag
    16381 cgatactgtt gatcgagttt gttgataatt ccggaaggta attctttatt atcaaataat
    16441 tggttacgca ttcgatagcg gtattcagcg ggtaaatcaa ccagtaaatc aatggcatta
    16501 tttaacatct cttcgtcaac aacaccatta tcaatcagcg ccagctgtac ttcgtaggct
    16561 tgctcttgat cttcttgccg tttgctcatc attaaattta atgtgcgttg ccataactgt
    16621 ttcttatatt ccagcggcaa taatttatcg cgtgccagct catcccagac ggtgggaaca
    16681 tcatgctgag tcagtagata actaattact tccctcgatg tcagataacg cgcaccttcg
    16741 cgcaaattgt gcacatcttc ttttgccaga gacagttgac gcatctccgg caacgctatc
    16801 aataacgcct ttacgatatt tttattttct ttattatcca gatacatttg ttctgcgata
    16861 cgttcaatat cctctgtacg cagggttgct gacagttggg taatttttaa ttcagtaagc
    16921 gtccgggcta atgcttcacg aactttcagt gatttatctt tactcaacat cacggcttgt
    16981 tgtgcggtta aatctgctcc ggtaattctt tcacgtaact ccgggtcttc gttatttatc
    17041 aactcatcgg ccactgcagt cggaaccagc caggcgtaat cgtaatggct gagtaatgtc
    17101 ttgcgtacac tgagaaaggg gctatgactt aacgacgcaa caatatcagg agaaagatta
    17161 tcctgtctgg cctgctccag ctgtacgctc tcgacgggta cagacattaa ttttgtattc
    17221 acatcggcgg aaatattact gtttttaacc agccctctgg caacagcctc atctttaatt
    17281 aattcatcgg taagcaaatg catctgttga gcgggccagg actgcgccat cgattcacgc
    17341 gtctggtcat cggggtgctg taacagttgc agcataacgg cgggtgataa actctctgta
    17401 tgagtcagaa aacgtgccac cagtggatct tgatgcttta ttttattcgc tgggaaatta
    17461 tcgagtttcc ccgtttcaat ccattttaat ttctcattaa tttcaggagc ggctttgtct
    17521 cgaaaaagcg gtaaaccctg aacgatgttg tctgcgacgc tatcatagtc aggatacgca
    17581 ttggaaatgt tatcggtttc gatttcagtg tgatcataaa gatcggcagt caccagtgaa
    17641 taggcttttt gaatattctc atcccagtta tatccttcac cacttccatc ttcattgata
    17701 taaaattcag ctttaatgac ttcaaattgc gttgctttat cggaaacaat aatgcctcgc
    17761 gcactaattc ccttcatcac ttcaaagtta tgatcattgt aatcagcata aactaaacct
    17821 ttcgctgtca tctgtccttt gacatacgcg aaatcatgat taataaagtt atcgacaata
    17881 acattaccta atacaatcag atgaccatta cctgacagat aatcatcata atcgccggaa
    17941 atcgttaaat taccgttcac caccagttta ttttgattgc tgaagttagc gggtaatgat
    18001 aggtcaccat tatgataata ccagcggata ttatctttta gcatataagc agcttccata
    18061 ctatcgtcgg ttgccgcttt aaccacgttg cgctcatctg ccgtcataag atgaaagtca
    18121 gaggtgataa gctcctcgct actgatccac tctgtggcgc atgcattgcc agtcataaaa
    18181 aatacgacca tacagatgac caatatatta attctattca taatcttcaa tgatatttat
    18241 atcaaccttg atgaattatg aatatatcgg aaattgaaaa aatcgcagca aaggaaatat
    18301 tattatgggt taaagagagg atatttcaga aaaatgccct cccgttcaag gggaaggcga
    18361 ggaattacag atagcggcac agataagagg tgggttcggc aacttgcaga tgaaactcac
    18421 tgtgaccggg aacattaaac accgaaccgg cttcatacac ttgccagtcg gtcgcgtcag
    18481 gcagtaacac attcagcgcg ccactgatta ccgtcatttc ttccggctca gcggtgctga
    18541 aggtgtattc gccttcaacc ataacaccca cgctggcgcg accagtgctg ctgctggaaa
    18601 agccgatcga tttcactttg ccggaaaagt actcattact ttgaagcata aactggccct
    18661 gtctggtagt tgaagatcta cttcactata ggggccagat gcgctggctg tcacgcaaaa
    18721 ttacaccagt aattccgcag ccagccgtgc aatcaatacg ttagagagca agacaggaac
    18781 atcgagctgt ttttgcagta aatcgcgatg acgctggtta aatcccaaac aatccagcat
    18841 gatgacatct gcgcctttcg ccagtaattc tttcccggca tcaatgattt tttgttctga
    18901 atcatgaatg gggttaccca atgaaaatac cggcggtttc tgcaaaattt gccatttttg
    18961 cgcctgaacg gtcagcaact cctcaaccgg tacgataacc cccacctgat gatcttcaac
    19021 aatagaggaa accagcggtg gcaatatccg cgacggctca agaaagatcg tattacgcgc
    19081 agtcatacta ctaatgtttg ccgtactcat taatataatg acgtcgtagc cctgattatc
    19141 gagcacttca accacacctt gcaggtcacg ctctactttg cgacgcgaaa catgggccag
    19201 atggttgtca tttaataatg tgagaattgt gtcttcgcct gcttctggcg cgtactccgc
    19261 catcacttct tcgcggctta acttccccag caggctatga tgggaaatat tatcttcgtc
    19321 aatgtattct gtcaggagcg gcaaaacttc ctgcataggt acaatgccga tggtcaggat
    19381 cgccaacgac gcactcattg ttcttccacc ttacttttat ttcgtcgtta attaccgggc
    19441 aagtgttaag caccactgct gacacttact tgtgctacaa atgacaaaaa gcgtagcagc
    19501 agacgtgcac ggcataatgt aaagattcca aatgattcca gtaatggatt gttattgttt
    19561 aatattctaa ttattagaaa aacatgaatt atgaaaaatg tgacgcagat cccttgtagt
    19621 ggcactcaga atcccttccg agcagtctga tgcggtcact ctttgtcagg gtcctcatag
    19681 cgttttttcg catccatcgc ttcctgagcg gtaggatgtt cactgatcaa cgagtctggt
    19741 ttaggatgat cggctctgag ttggtaccag gttaccgtct gtcctggctt tcctttttca
    19801 atcgtcacta tatatgcttc acgaggataa ggcggtttcg ttggcatcgt tcttccttat
    19861 ttcacgggat gaacgttaag tataggcgct cgaaaatcaa caattgatcg tctgtgccag
    19921 ggcgctgcga atttcagaaa tcacctggct gggttcgttt gttgcgtcga tgataatatg
    19981 cgcaacttcg cgatatagcg catcgcgttc ttccagcact tcctgaactt cttcgctcag
    20041 cggttttccc gttaaggttg gccgtaaatc ttcttccggt gcagcttgca gtcggttaac
    20101 caggactgat actggcgcac acaaataaac cacgatcccg ttattttgca tgaagtgacg
    20161 attaaattcc gtcagaataa tgccgccgcc tgtagcgata acggtggatg gcgcagttac
    20221 cgcttccagc gccgccgttt ctctggcgcg aaatcccgcc cactcttccc tttcgacgat
    20281 ctccgcgacc gtcatattga gctgtgattg caaccactga tcggtatcga caaaccgacg
    20341 attaagcgaa tcggcaaggg ccattccgac cgttgtttta ccacagcccc gaggcccgat
    20401 cagaaaaaga ggttgtgtca tcgtgggttt tccccaatag gtcgcaatgc ggcgaaagcc
    20461 ggtgtcatga gaatagcgat cataccatca aactagtaca atttcgattg taaagaaaaa
    20521 attccactta aagtgaaaat ctcaatacat cccttactat accaataaat attcaagaat
    20581 gaagtgtaaa taataaatta catttagcca cgactacgtt gcacttccag ccaccacttc
    20641 tccagctccg cggcaaaggc ctggcggtca cgttgtgaaa ggctatctgg tccgccggtc
    20701 tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg ctcacgaatg
    20761 gtcgctggcg tgtaacgttc accgcgcgga ttaagcgccg cagcgccttt ctcgatggct
    20821 tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca ctgccggaca
    20881 atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca gcgtacgaat aaatcgcgat
    20941 ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat acgttccgcc
    21001 gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac ccaaattgtc
    21061 ataaagtcat cctttgttgg gtaatcctct attgtgtcgc gcttttgcct tccggcatag
    21121 ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca ggagtgaggc
    21181 aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca ttctcggcgg
    21241 tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatacaccc cctccccgga
    21301 taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg cgcaagaagt
    21361 ggcgcaaatc gccgacatca tttttgctgc cgttaaacct ggcatcatga ttaaagtgct
    21421 tagcgaaatc acctccagcc tcaataaaga ctctctggtc gtttctattg ctgcaggtgt
    21481 cacgctcgac cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc gcgccatgcc
    21541 gaacactccc gcgctggtta atgccgggat gacctccgta acgccaaacg cgctggtaac
    21601 cccagaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag cggaagtaat
    21661 tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag cctacgtctt
    21721 tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac gtgcccaggc
    21781 gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg aaacgggaga
    21841 acatccaggg gcactgaaag atatggtctg ctcaccggga ggcaccacca ttgaagcggt
    21901 acgcgtactg gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga cgaagtgtat
    21961 ggaaaaatca gaaaaactca gcaaatcctg atgactttcg ccggacgtca ggccgccact
    22021 tcggtgcggt tacgtccggc tttctttgct ttgtagagcg ccaaatctgc cgatttcaac
    22081 cactcacgat agtgactcat ttgtgggttc agcggcgcaa cccccacact aatccgtaac
    22141 atcacctgcg gcgtattcgg caggcgtaac gtatttaatc cttcatgcac tcgtaacatg
    22201 gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ctgcaaactc atcgccgcca
    22261 aaccgaccaa tcacatcgct accgcgcagg gtaatttgta actgtcgggt aagcgccaca
    22321 atcgcttcat cgcccacatc atggccccag gtatcgttga tgctcttgaa atggtcgata
    22381 tcgataatca gtaacgttgc atcgcgatta tgccgccgac agttatcaaa ttcattgcgt
    22441 aacatagttt cccaatgacg tcggttatac acgcccgtca tgccgtcgcg ggtactcatg
    22501 acctgcaacc tgcgtttatg ttccgccagt ttagtcgccg tctggtagct gacccagcca
    22561 aacagcagag gataaatgac aataatggga agggagagcc accattccag cggcgcacta
    22621 ttgaacgaca cggtgatgcc cgtcagctcg agggtgacaa ggcaggaaac caccatcaac
    22681 accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag acacataatc
    22741 atcaacatcg cggtggaagg cagcacgttt acgcccatta cgcctaccca cattcccgct
    22801 aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac ggccctgctc
    22861 gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac cagccaccac
    22921 cagcccggcg gcgggtgtga aaccagcgtt gaagcaatcg gtaagaacat gccagccagg
    22981 ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg
    23041 ttttgaggag ttaaaggagg ttcctccccg tgcgccgccg cttttttgaa aaagttttca
    23101 tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga gacggggcta
    23161 aaggcagaag tatgaaattt cgggtgagcc attttagtag ctcacccgtt agcactatca
    23221 ggtatcaggc ggctttcttg aggcaggcgc tcataaactt attacgatca tcacctttca
    23281 gtgattgttg cgttgcttga ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac
    23341 ttttttcacc aggcgcagac ttgctgttct tcaggcaatc actcatgtag gtcttacgag
    23401 catccccttt caacgcctgt accgtcgcct gctgattaca ggaggtcata cgctgttgtt
    23461 gtggggttaa ggttctctcg gcagcgccga cggtggttaa aaaaaccaga ccgaaaagca
    23521 aggtaaccag taatgtaatt ttcatagcac catccctctt catgttttaa ccatgagcgt
    23581 atacgcccgt gatctgccat taagtctggt tgctaacagc aaaaaaacca cccggcagcg
    23641 aaaattcact gccgggagcg gttttatttc agccccagag cggctttcat ggtgtagaag
    23701 agatcggtct ggtcggtcag tccaacgaca ttggcggcat gcgggccata cgccgcaata
    23761 cgcaactgac tgccggtatg ttcttgtgaa tcctcttcgg agttcccgta actcatcacc
    23821 atcactgcgc catctttggt atttagcgcc tgggtgaggc ccggagcttt ggtatccggc
    23881 gcaacaatct ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc
    23941 tttttggcga attccagcgc ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt
    24001 tgcccacaag gattcgcagc atgatcctgt ttatcgattg acgcaccttc aacttgcagg
    24061 aaaaagcctt tctcattttt actcaacaat tcaatggctt tgtcggtcat ctgtgccagg
    24121 gtcggtacac tgtcattacg ttgcggatta ggcgtacagg tgactgcggg cttatcgata
    24181 ttaccgtggt acgttgcttt cggtcctagc cagcgcactg gcatattgcc gtcagcaaac
    24241 aggccaagca ggggtttttg ctgattcgct tccgtcaccg aattcagtga ggcagcatcg
    24301 ctcaccaact gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca
    24361 ccagcggttg ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac gtcggcacga
    24421 gcgttaagca gctgttcggt aatcgatcct tttccgcctt tttccagagc gttacccgga
    24481 catttttcac tggtcgcgct cggaccgtag catttgcgcg aggtcacatg cgccaccagc
    24541 gcggcgggcg tggcatcctg caactctgcg gtagaaacgt taccggtcgc cagacctgcg
    24601 gcttttgcca tttccagaat cgttgggtga tctttttcgt gaatatcgac gcccagcgcg
    24661 ccgttatagg ttttgacacc ggttgaccag gcggttgctg atgcagccga gtcggtgacg
    24721 tagtccggtt tgccggtttt tttattcagc gcatagtgag tgtattgccc ggtaagcggt
    24781 aaggcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg tgcggcagta
    24841 atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc aggtttatcg
    24901 ctaagagaat cacgcagagc ggcggtctga tcagccgtta agcggcgagc accgccgggt
    24961 gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg tgtccgggct
    25021 tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct ttgtttcact
    25081 ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat aagtctcggc
    25141 cgtgacaact ttatgacagc tgttgaaaag attaactttt tactgacgag gatgcttcaa
    25201 taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg
    25261 ctttacctcg tacagcgccc cctctacctg atcaatcaac cgttgttggt cattttgcgc
    25321 catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg cacacagttc
    25381 tttcgactct tcttcttttt gggcaagctt aaataacaac tcagcaatga gatttttcat
    25441 gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca acttcatagt
    25501 gtctgg
//
LOCUS       DAPGRN010000067        24161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPGRN010000067 DAPGRN010000000
VERSION     DAPGRN010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.067"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="RHW33_002892"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="RHW33_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV1646376.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="RHW33_002893"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="RHW33_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV1646377.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLTVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="RHW33_002894"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="RHW33_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV1646378.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="RHW33_002895"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="RHW33_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV1646379.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="RHW33_002896"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="RHW33_002896"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV1646380.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="RHW33_002897"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="RHW33_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV1646381.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="RHW33_002898"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="RHW33_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV1646382.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /locus_tag="RHW33_002899"
     CDS             complement(7754..8632)
                     /locus_tag="RHW33_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV1646383.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQAWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLV"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="RHW33_002900"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="RHW33_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV1646384.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQRAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFTHVAPIDCLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQMLLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQIEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALKAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTACMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            11259..11594
                     /locus_tag="RHW33_002901"
     CDS             11259..11594
                     /locus_tag="RHW33_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV1646385.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            11959..12402
                     /gene="yjdN"
                     /locus_tag="RHW33_002902"
     CDS             11959..12402
                     /gene="yjdN"
                     /locus_tag="RHW33_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV1646386.1"
                     /translation="MPLSPYISFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            12535..13323
                     /gene="phnC"
                     /locus_tag="RHW33_002903"
     CDS             12535..13323
                     /gene="phnC"
                     /locus_tag="RHW33_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV1646387.1"
                     /translation="MQTIIRVEQLSKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLIVGDKKPECRVELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRVEENAKAA"
     gene            13348..14364
                     /gene="phnD"
                     /locus_tag="RHW33_002904"
     CDS             13348..14364
                     /gene="phnD"
                     /locus_tag="RHW33_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1646388.1"
                     /translation="MNAKIIASLAFTSMFSLSTLFSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLARRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            14470..15249
                     /gene="phnE"
                     /locus_tag="RHW33_002905"
     CDS             14470..15249
                     /gene="phnE"
                     /locus_tag="RHW33_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV1646389.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMLVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRCLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            15270..15995
                     /gene="phnF"
                     /locus_tag="RHW33_002906"
     CDS             15270..15995
                     /gene="phnF"
                     /locus_tag="RHW33_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV1646390.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FAVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGIKEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKESQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            15996..16448
                     /gene="phnG"
                     /locus_tag="RHW33_002907"
     CDS             15996..16448
                     /gene="phnG"
                     /locus_tag="RHW33_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV1646391.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            16445..17029
                     /gene="phnH"
                     /locus_tag="RHW33_002908"
     CDS             16445..17029
                     /gene="phnH"
                     /locus_tag="RHW33_002908"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV1646392.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            17029..18093
                     /gene="phnI"
                     /locus_tag="RHW33_002909"
     CDS             17029..18093
                     /gene="phnI"
                     /locus_tag="RHW33_002909"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV1646393.1"
                     /translation="MYVAVKGGEKAIDAAHALQENRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVCEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            18086..18931
                     /gene="phnJ"
                     /locus_tag="RHW33_002910"
     CDS             18086..18931
                     /gene="phnJ"
                     /locus_tag="RHW33_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV1646394.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     VIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            18928..19686
                     /gene="phnK"
                     /locus_tag="RHW33_002911"
     CDS             18928..19686
                     /gene="phnK"
                     /locus_tag="RHW33_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV1646395.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYGMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            19797..20477
                     /gene="phnL"
                     /locus_tag="RHW33_002912"
     CDS             19797..20477
                     /gene="phnL"
                     /locus_tag="RHW33_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV1646396.1"
                     /translation="MINVQNVSKTFILHQQNGVHLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            20474..21610
                     /gene="phnM"
                     /locus_tag="RHW33_002913"
     CDS             20474..21610
                     /gene="phnM"
                     /locus_tag="RHW33_002913"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV1646397.1"
                     /translation="MIINNVKLVLENEVVHGSLEIQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLMQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAV
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            21610..22167
                     /gene="phnN"
                     /locus_tag="RHW33_002914"
     CDS             21610..22167
                     /gene="phnN"
                     /locus_tag="RHW33_002914"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV1646398.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYASAMLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            22154..22588
                     /gene="phnO"
                     /locus_tag="RHW33_002915"
     CDS             22154..22588
                     /gene="phnO"
                     /locus_tag="RHW33_002915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV1646399.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            22590..23348
                     /gene="phnP"
                     /locus_tag="RHW33_002916"
     CDS             22590..23348
                     /gene="phnP"
                     /locus_tag="RHW33_002916"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV1646400.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACVRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRENAPRNHCDLNTVLALNQVIRSPRV
                     VLTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(23425..23753)
                     /gene="yjdP"
                     /locus_tag="RHW33_002917"
                     /pseudo
     CDS             complement(23425..23753)
                     /gene="yjdP"
                     /locus_tag="RHW33_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
     gene            complement(23822..24139)
                     /locus_tag="RHW33_002918"
     CDS             complement(23822..24139)
                     /locus_tag="RHW33_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDV1646401.1"
                     /translation="MQPVFFIKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEGIDKET
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         5625 a         6863 c         6416 g         5257 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc attctcaccc tgccatgcct gacaccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc cactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccatcggt ggggtattga ttgccgccgt ttgctatgtg
     1681 ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcctcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cattgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagaattc ggtctggttt cttccgtgtc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctgacagtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaagat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggac tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgccttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cgctggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggtgtg cttgctgcgc tgattgcctg
     2821 ctggattaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactgc tgattttgct ggtcgccgca ctattttata aagactacgc
     2941 ctcgttgttc cgcaacaaca aagagctggt gaagtcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctgccgctgg tgcgaattgg
     3061 tgaagacgcg caccgcaacc cattaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 tgtcaccgcg ctgaatctgc ctgatcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtacc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgagtcgtt
     3781 aggtgaaaat ggcatctatc tgcacggttt gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgtctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggactga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggcaggt cattacagct tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atataccctg
     4261 ccggtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgccgg gctggatgtc
     4321 ggtgccgacg actatctggt gaagcctttt gcgctggaag agttacatgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaacatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgcttg tcgcgaatga ggaaaactaa ttgaatctga tgcgttttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tacgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaatc tggtagaaaa tgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gccgttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagttta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc tggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcagaccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcgctcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tatttagtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcaac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcgataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tctcttcgcc gacaatggtc gaaattaaca ccactacgcc agctcccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggggagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gtccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc atcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttagtg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacacc aaatttacag agaagatttc ctcgagcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggaccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg gcaggcatcg ccaaatgccc
     8101 cccagcgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgttc acctgcctgc accagtaggc
     8221 cccgttgcgt taacgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgttgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc aaacgcaggc taagcggcgc atttaacatc ccgccgttcg
     8461 tccatgcctg aagctgcaca cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcgcg
     8581 gatgaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctga gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ttgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcatgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaacc gtctctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt caagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
     9361 cttcagcgca tcttgctgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgatctgg tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacatctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcaa ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgccc gatttgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgagcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactc tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcaccaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattttt gaattaaaca atcgattggc gcgacatgtg taaaatgcag
    10501 taccggttct ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attgcgatta ggcagtacct cggtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatagta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgct cggcgatatt aggctgactc cagggtaaag cccgttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttattact tttatttagc caaattgttt ttgagttctg
    10921 ttttcggctt ttataattac tgcaagaaat aattttatat ttagagtgtt gttttttatt
    10981 agaataaata acatcttccg atacgtttaa aacgtcagaa agataaaaat atcatgtgaa
    11041 taaaaaaaag aacaagtaga gcattaacct tatcttaaat aataaatgga ggcaaaaaga
    11101 ttattttctt tttgcgtttc ctttcaaatg aaaacgatcg tcgtctaaaa tcagcagtac
    11161 ccccgacaaa ctcagggatt ttgtgtataa ttgcggcctt tttcggcaat ctgccgtttt
    11221 ttggcgcttt tgccctgctg acttttgagg aaatccacat gtcattacca cactgcccaa
    11281 aatgcaactc cgaatacacc tacgaagata acggcatgta catctgcccg gaatgtgcct
    11341 acgaatggaa cgacgcagaa cctgcacagg aaagcgacga gctgatcgtt aaagatgcta
    11401 acggcaatct gctggctgac ggcgacagcg ttaccatcat taaagatctg aaggtgaagg
    11461 gtagctcttc gatgctgaaa attggcacca aagtgaaaaa catccgcctg gttgaaggcg
    11521 accataacat cgattgcaaa atcgacggtt ttggtccgat gaaactgaaa tctgagtttg
    11581 tgaaaaagaa ctgattgtat tgtgatcggt aagccggata aggcgtttac gccgcatccg
    11641 gcaacggtgc cagatgcctg atgcgacgct tgcgcgtctt atcaggccta caaattcccg
    11701 caccctccgt aggccggata aggcgtttac gtcgcatcag gcaacagtgc cagatgcctg
    11761 atgcctgatg cgacgctcgc gcgtcttatc aggcctacaa attcccgcac cctccgtagg
    11821 ccggataagg cgtttacgcc gcatccggca acagtgccga ctgcctgatg cgacgctcgc
    11881 gcgtcttatc aggccgcctc tcatctgtat aaatttcgaa ctacacttaa ctggcttctc
    11941 ttcactgagg tcaccatcat gccgttaagt ccctacatct cttttgccgg taactgttcc
    12001 gacgcgattg cctattatca acgcacgttg ggcgcggaac tgctctataa aatcagcttc
    12061 ggcgaaatgc caaaatcagc gcaggacagc gccgagaact gcccttccgg aatgcaattt
    12121 cccgataccg ccatcgctca tgccaacgtg cgcattgccg gaagcgacat catgatgagc
    12181 gatgccatgc cgtcaggaaa agccagctac tccggcttta cgctggtgct cgattcgcaa
    12241 caggtcgaag aaggaaaacg ctggtttgat aaccttgccg ctaacggaaa aatcgaaatg
    12301 gcctggcagg aaactttctg ggcgcatggc ttcggcaaag tcaccgataa atttggcgta
    12361 ccgtggatga ttaatgtcgt caaacaacaa ccaacgcaat aatccgccgg gaggcccgcc
    12421 ctcccgcact gtcatcgaat tcccgttaac tcttcatctg ttagtcactt ttaattaacc
    12481 aaaccgtcac aataatccgc cacgatggag ccactttttt agggaggctc cagcatgcaa
    12541 acgattatcc gcgtcgaaca actgagcaaa accttcaatc agcatcaggc gctgcacgcg
    12601 gttgatctga atatccatca tggtgaaatg gtggctctgc ttgggccgtc cggttccggc
    12661 aaatccaccc ttttacgtca cttaagcggc ctgattgtcg gcgataaaaa gccagaatgc
    12721 cgcgtcgaac tgctgggccg cactgtccag cgcgaaggcc gtctggcccg cgatatccgc
    12781 aaaagccgcg ccaacaccgg ctacatcttc caacaattca acctggtgaa ccgcctgagc
    12841 gtactggaga acgtgctgat tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt
    12901 agttggttca cccgcgagca gaaacagcgc gcgttacagg cgctgacccg cgttggcatg
    12961 gtgcattttg cccatcagcg cgtttccacc ctctccggcg gccagcagca gcgtgtagcg
    13021 attgcccgcg cgctgatgca gcaggcgaag gtgattctcg ccgacgaacc gatcgcctct
    13081 ctggacccgg aatcggcgcg catcgtgatg gacaccctgc gcgacatcaa ccagaacgac
    13141 ggcatcaccg tggtcgtcac gctacatcag gtggattacg ccctgcgcta ctgcgaacgc
    13201 atcgtcgccc tgcgtcaggg gcacgtcttc tacgacggca gcagccaaca gtttgataac
    13261 gaacgctttg accatctcta ccgcagcatc aaccgcgtcg aagagaacgc gaaagctgcc
    13321 tgacatcccc atcattgagg aaaacgaatg aacgctaaga taattgcctc gctggccttc
    13381 accagcatgt tcagcctcag caccctgttc agcccggcgc atgccgaaga gcaggaaaag
    13441 gcgctgaatt tcggcattat ttctacggaa tcacagcaaa acctgaaacc gcaatggacg
    13501 ccgtttttac aggatatgga gaagaagctg ggcgtgaagg tcaacgcctt ctttgccccg
    13561 gactacgcgg gcattatcca gggaatgcgt ttcaacaaag tggatatcgc ctggtacggc
    13621 aacctgtcgg cgatggaagc ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc
    13681 gcggcggatg gatcgccggg ttactggagc gtgttgatcg tcaacaaaga cagcccgatc
    13741 aacaacctga acgatctgct ggccaggcgg aaagatctca ccttcggcaa tggcgatcct
    13801 aactccacct ctggcttcct cgtccccggt tactacgtct ttgccaaaaa caatatctcc
    13861 gccagcgact ttaagcgcac cgtcaacgcc gggcatgaaa ccaatgctct cgccgtcgcc
    13921 aacaagcagg tggatgtggc gaccaacaac accgaaaacc tcgacaagct gaaaacctcc
    13981 gcgccggaga agctcaaaga gctgaaagtg atctggaagt cgccgctgat cccaggcgat
    14041 ccgatcgtct ggcgcaagaa tctgtccgaa accaccaaag acaagatcta cgacttcttt
    14101 atgaattacg gcaagacgcc ggaagagaaa gcggtgctgg aacgcctggg ctgggcgccg
    14161 ttccgcgcct ccagcgacct gcaactggtg ccgattcgcc agctcgcact ctttaaagag
    14221 atgcagggcg taaaaagcaa taaaggactg aatgagcagg acaagctggc aaaaaccacc
    14281 gagattcagg cgcagctgga tgacctggac cgcctgaaca acgcgttaag cgcgatgagt
    14341 tcggtgagta aagcggtgca gtaaatcgta ggtcggataa gacgccccgg cgtcgcatcc
    14401 gacaatgtgc aggcgttgat gccggatgcg gtgcaagcac cttatccggc ctacagaccg
    14461 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gcagctggtt ctcgcttctg
    14521 agctgggccg ttgtactcgc cgtgttggtc gtctcgtggc agggcgcgga aatggccccg
    14581 ctcacgctga ttaaagacgg cggcaacatg gcgactttcg ccgccgactt cttcccgccc
    14641 gatttcagcc agtggcagga ttacctcacc gaaatgctcg tcacgctgca aatcgccgtc
    14701 tggggcaccg cgctggcggt ggttctctcc atccccttcg gcctgatgag cgccgaaaac
    14761 ctggtgccgt ggtgggtgta ccagcccgtg cgctgcctga tggacgcctg ccgcgcgatt
    14821 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gcctcggccc gttcgctggc
    14881 gtgttggcgc tgttcattca caccaccggc gtgctctcca aactgctttc cgaagcggtg
    14941 gaagcgattg aacccggccc ggtggaaggc attcgcgcca ccggcgccaa caagctcgaa
    15001 gagatcctct acggcgtgct gccgcaggtg atgccgctgc tgatctccta ctctctctat
    15061 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggcgc gggcgggatc
    15121 ggcgtcacgc tgtgggaagc gattcgtggt ttccagttcc agcaaacctg cgccctgatg
    15181 gtgctgatta tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
    15241 tttatctgat tagcgaggca ttgatagcta tgcacttgtc tacacatccg accagctacc
    15301 caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactaccgct
    15361 gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg ctttgcggtg aaccgccaca
    15421 ccctgcgtcg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggcg
    15481 tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
    15541 gccagaatct gctggatcag ggcagccatc ccaccagcga aaaactgctg tcggtgctgc
    15601 gcccggcctc cggtcacgtc gctgacgcgc tggggatcaa ggagggcgag aacgttatcc
    15661 acctgcgcac cctgcgccgg gtcaacggca tcgcgctctg tttgatcgac cactacttcg
    15721 cggacctcac cctctggccg acgctgcaac gcttcgacag cggctcgctg cacgattttc
    15781 tgcgcgagca aacggggatt gcactgcgcc gcagccagac gcggatcagc gcccgccgcg
    15841 cccaggccaa agagagccag cgtcttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc
    15901 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
    15961 gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc
    16021 agcactggat gtccgtactg gcgcacagcc aacctgcaga actggcagcc cgcctgaacg
    16081 cgctgaacat caccgccgac tatgaggtga tccgcgccgc tgaaactggc ctggtacaga
    16141 ttcaggcgcg gatgggcggc accggcgaac gcttttttgc cggcgacgcc acgcttaccc
    16201 gcgccgccgt acgcctgact gacggcacgc tcggctacag ctgggtgctg gggcgcgaca
    16261 aacagcacgc cgaacgctgc gcgctgattg acgcgctgat gcagcaatct cgccactttc
    16321 aaaacttatc agaaaccctt attgccccgc tggacgctga ccgtatggca cgcattgccg
    16381 cacgccaggc cgaagtaaac gccagccggg tcgacttctt tacgatggtt cgcggagata
    16441 acgcatgacc ctggaaaccg cttttattct tcccgtgcag gatgcccagc acagttttcg
    16501 tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgcatc aactcaaacg
    16561 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg cggataacga
    16621 cacgccggtg tggcttgccg cgccattaag taacgatatc gtcaaccaga gcctgcgttt
    16681 tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg tgacggatga
    16741 cggaatttcc agcgaacagc tcaacgccct ttccaccggc accgccgttg caccggaagc
    16801 gggcgcgacg ctgattttac aggtcgccag cctgagcggc gggcgcatgt tacgcctcac
    16861 cggcgcgggc attgccgaag aacggatgat cgccccgcaa ctgccggagt gcattctgca
    16921 cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctgatcc tgacctgcgg
    16981 cgagcgcctg ctggctatcc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc
    17041 gtgaaagggg gcgagaaggc gatcgacgcc gcccacgccc tgcaagagaa ccgacgccgg
    17101 ggcgataccg atttgcccga actgagcgtc gcccaaattg aacagcagct taatctcgcg
    17161 gtggatcgcg tgatgaccga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
    17221 aaacaggcca gcggcgataa cgtcgaagca attttcctgc tgcgcgccta ccgcaccacg
    17281 ctggcgaagc ttgcggtatg cgagccgctc gacaccacgg agatgcgtct cgaacggcgt
    17341 atttcagcgg tttataaaga tattcccggc ggccagttgc ttggcccaac ctacgactac
    17401 acccatcgcc tgctcgattt caccctgctg gcaaacggcg aagcgccgac gctgaccacc
    17461 gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
    17521 gcgaagtttg aagaagatag cggcgcacag ccggatgaca tcacccgcac gccgccggtt
    17581 tatccctgct cacgttcttc ccgtttgcag cagttgatgc gcggcgacga aggctatttg
    17641 ctggcactgg cctactccac ccagcgcggt tacggacgca atcacccgtt cgcgggcgag
    17701 atccgcagcg gctatatcga cgtatcgatt gtgccggaag agctgggatt tgcggtaaac
    17761 gtcggcgaac tgctgatgac cgagtgtgaa atggtcaacg gttttatcga cccgccggat
    17821 gaaccgccgc acttcacgcg cggctacggg ctggtgttcg gcatgagcga gcgcaaagcg
    17881 atggcgatgg cgctggttga ccgcgctctg caagccccgg agtacggcga gcacgcgaca
    17941 ggcccggcgc aggatgaaga gttcgtgctg gcacatgccg acaacgtcga agccgcaggc
    18001 tttgtctcgc acctcaaact cccgcactac gtcgatttcc aggccgaact ggagctactc
    18061 aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct
    18121 cgacgagcag accaaacgca tgatccgccg cgccatttta aaagcggtgg cgatccccgg
    18181 ttatcaggtg ccgtttggcg ggcgcgagat gccgatgcca tacggctggg gaaccggtgg
    18241 catacaactc accgccagcg tgattggcga aagcgacgtg ttgaaggtga ttgaccaggg
    18301 cgcggatgac accaccaacg ccgtatcgat tcgcaacttc tttaagcgcg tgaccggggt
    18361 aaacaccacc gagcgcacgg acgatgcaac ggtcatccag acgcgtcacc gcatccccga
    18421 aacgccgctg accgaagatc agataattat tttccaggtg ccgatccccg aaccgctgcg
    18481 ctttatcgag ccgcgcgaaa cggaaacccg caccatgcac gcgctggagg agtacggcgt
    18541 gatgcaggtg aagctgtatg aagatatcgc ccgcttcggt catatcgcca ccacctacgc
    18601 ctatccggtg aaggtaaacg ggcgctacgt gatggacccg tcgccaatcc cgaaattcga
    18661 taacccgaaa atggacatga tgcccgccct gcaactgttc ggcgcagggc gcgagaagcg
    18721 catctatgcg gtgccgccgt ttacccgcgt ggaaagcctc gatttcgacg atcacccgtt
    18781 caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga
    18841 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
    18901 ccgccaacag agcgaggcaa aaagccaatg aatcaaccgt tactttcggt caataacctg
    18961 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg
    19021 gaagtgctgg gcattgtcgg ggaatccggc tccgggaaga ccacgctgct gaaatcaatt
    19081 tccgcgcgtc tgacgccgca gcagggggag attcactatg aaaaccgttc cctctatggc
    19141 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat
    19201 ccgctcgacg gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg
    19261 gcgaccgggg cacgtcatta cggcgatatt cgcgccaccg cgcagaagtg gctggaagag
    19321 gtggaaattc ccgccaaccg gatcgacgac ctgccgacca ccttttccgg cggtatgcag
    19381 cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtatt tatggacgag
    19441 ccaaccggcg ggctggatgt gtcggtgcag gcgcgcctgc tcgacctgct gcgcggcctg
    19501 gtggtggagc tgaacctcgc ggtagtgatt gtcacccacg atttaggcgt cgcccgcctg
    19561 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
    19621 cgcgtgctcg acgacccgca ccatccgtat acacaactgc tggtgtcatc ggttttgcag
    19681 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
    19741 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
    19801 ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc gtgcacctgc
    19861 ccgtcttaca acgcgcctca ctcaccgtca acgcgggcga atgcgtggtg ctccatggcc
    19921 actccggcag cggaaaatca accctgctac gctcgctgta cgccaactat ctgcccgatg
    19981 aagggcaaat ccagatcaaa cacggtgacg agtgggtaga cctggtcacc gcgccagcgc
    20041 gcaaagtggt ggagatccgc aaaaccaccg tcggctgggt gagccagttt ctgcgcgtca
    20101 tcccgcgtat ctcagcactg gaagtggtga tgcagccgct gcttgatacc ggcgtaccgc
    20161 gtgaagcctg cgccgctaaa gccgcgcgtc tactcacccg cctgaacgtg ccggaacgcc
    20221 tgtggcgcct ggcgccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc
    20281 gcggctttat cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg
    20341 ccaaaaacag cgccgccgta gtggaactga tccgcgaagc caaagcccgt ggcgcggcca
    20401 tcgtcggcat cttccatgac gaagccgtac gcaatgacgt cgctgaccgc ctgcacccaa
    20461 tgggagcctc tgcatgatta tcaataacgt taagctggtg ctggaaaacg aagtggtgca
    20521 cggttcgctg gagatacagg acggtgaaat ccgcgccttt gccgaaagcc agagccgcct
    20581 gccagaagcg atggacggcg aaggcggctg gctgctgccg ggcctgattg agctgcatac
    20641 cgataacctc gataaattct tcaccccgcg cccgaaagtg gactggcccg cccattcggc
    20701 gatgagcagc cacgacgcgc taatggtggc aagcggcatc accaccgtac tggacgccgt
    20761 ggcgattggc gacgtgcgcg acggtggcga tcggctggag aatctggaga agatgatcaa
    20821 cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgtc tgcaccttcg
    20881 ctgcgagctg ccacaccaca ccacgctgcc gctgtttgaa aaactgatgc agcgcgagcc
    20941 ggtgacgctg gtgtcgctga tggaccactc gccaggccag cgccagttcg ccaatcgcga
    21001 gaagtatcgc gaatattatc agggcaaata ctcgctcacc gacgcgcaga tgcagcagta
    21061 tgaagaggaa cagctggcgc ttgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc
    21121 cgccgtgtgc cgcgcgcgaa aaattgcgct tgccagccac gatgacgcca cgcacgccca
    21181 cgttgccgaa tcccaccagc ttggcagcgt gatcgccgaa tttcccacca cgtttgaagc
    21241 agcggaagcc tcgcgcaagc atggcatgaa cgtgctgatg ggcgcgccaa atatcgtgcg
    21301 cggcggttcg cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga
    21361 tatcctctct tccgactact accccgccag cctgctggat gcggcgttcc gcgtcgccga
    21421 tgacgagagc aaccgcttta cgctaccgca ggcggtgaag ctggtgacca aaaatccggc
    21481 ccaggcgttg aatcttcagg atcgcggggt gattggcgag ggcaaacgcg cggacctggt
    21541 gctggcgcat cgcaagggca accacattca tatcgaccac gtctggcgtc agggtaaaag
    21601 ggtgttctga tgatgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagac
    21661 agcctgctgg cggaactccg cctgcgggaa caaactcagt tactggtggc gcatcgctac
    21721 atcacgcgcg acgccagcgc cggaagtgaa aaccatatcg ccctgagcga gcaggagttt
    21781 tttacccgcg cggggcaaaa cctactggcc ttaagctggc acgccaacgg cctgtattat
    21841 ggcgtcggcg tcgagatcga cctctggctg cacgccggat tcgacgtgct ggtcaacggc
    21901 tcacgcgccc atctgccgca ggcgcgggcg cgctatgcat cagcgatgct gcccatctgc
    21961 ttacaggtgt cgccggagat cctgcgccag cgcctggaaa accgtggtcg tgaaaacgcc
    22021 agtgaaatta acgcccggct ggcgcgcgct gcccgctata ctccacagga ttgccatacg
    22081 ctcaataatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gattcatcag
    22141 aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtacg acaccgacgc
    22201 ggtttacgcg ctgatttgtg agctaaaaca ggcggagttt gaccatcacg cgtttcgcgt
    22261 gggctttaac gccaacctgc gtgacccaaa catgcgctac catctggcgc tgctggatgg
    22321 cgaagttgtc ggcatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat
    22381 cggcgaaatt caggagctgg tggtgatgcc gcaggcgcgc ggtctgaacg tcggcagcaa
    22441 gttgctggcg tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgaactttc
    22501 caccaacgtg aagcgccacg acgcgcaccg tttctacttg cgcgaaggct acgagcagag
    22561 ccacttccgc ttcacgaagg cgctgtaaca tgagcctgac cctcacgctc accggcaccg
    22621 gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc ggcctgcgtc agagcgcggc
    22681 gctcaccgca gtatcgccgc caaccgtgca gcggcgtagt gaagtttaac gacgcaatca
    22741 ccctgatcga cgccgggcgg cacgatctcg ccgatcgctg gtcgcccgga tcgttccagc
    22801 agtttttgct gacgcactac catatggatc acgtgcaggg gctattcccg ctgcgctggg
    22861 gcgtgggcga tccgatcccg gtttacggcc cgccggatga acagggctgc gacgatctgt
    22921 ttaaacatcc gggcctgctt gatttcagcc acacggtgga accgtttgtg gtgtttgatt
    22981 tgcaggggtt acaggtcacg cccctgccgc tcaaccactc aaaactgacc ttcggttatc
    23041 tgctggaaac ggcacacagc cgggtggcgt ggctgtctga caccgcaggc ttgccggaaa
    23101 aaacgctgaa atttttacgc aataatcagc cgcaggtaat ggtgattgat tgcagccacc
    23161 cgccgcgcga gaatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc
    23221 aggttatccg ctcgccgcgc gtggttctga cccatatcag ccaccagttt gatgcgtggc
    23281 tgatggaaaa cgcactgccg tcagggtttg aggcggggtt tgatgggatg gagattgggg
    23341 tggcgtgatg agagggaatg tgcgtgctgg ccccctcacc ctaaccctct cccctgaggg
    23401 gcgatgcctg ctccataccc aacatcattg cccatactca tcttccattc tccgctcttc
    23461 atcttctagc tgccgacgct cctgatcaag ctggtgctgg cgatcgtcta gttgcctgcg
    23521 gcgatcttct aactggcggc ggcggtcgtc atattgtctg cgccgatcgt cgctcacttc
    23581 acgctgccag ccgtcgtcgc gcgaatcttc atagtcacgc ccacggtcag ggttataagc
    23641 gtcgttaatc gcctgctgaa tattgccaat ggtgtcgtcg ataatatcgg cctggccggt
    23701 acggtggtca gcgtgagcag ggtgaataaa agaaatagcg gaaagcgttt cattagccaa
    23761 cctcaaaaag aaactctatc cacattagtc attactcatc catgcaagta gtggatgaat
    23821 ctcaatttct ccgctgctct attgccgtaa tcgcctccac gcgttgttga tgacgaccgc
    23881 cttcgtactg tgcgcccagc cacgcatcca caatcatttt tgccagttcg aggccaacca
    23941 ctcgtgaacc aaaagccagt gcaacttcac tggcgtaatg cggataatca gtgcgctctg
    24001 acgacctggt ttctttatca atcccctcac tcattgacca tgaataaaag gctggcacag
    24061 caacacagaa tcctgcggtg ccagcgacat gaccgcccat tgctccggta cgttttttaa
    24121 taaaaaacac cggttgcatt gattaattgt gctcagtatt t
//
LOCUS       DAPGRN010000068        23715 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPGRN010000068 DAPGRN010000000
VERSION     DAPGRN010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.068"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..688
                     /locus_tag="RHW33_002919"
     CDS             <1..688
                     /locus_tag="RHW33_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646402.1"
                     /translation="SLLDLDLFRGISSNYRVVVQLLISSCLSGDSDTIYDIQEELPFS
                     WGWIPVSIWKDVFQKCWTYLEKQINDKTLALHILQPFIAFMNHRAHIDRRLAPIANML
                     LTYSESLPTGCDVLPTVSREQFNEAKQMLLRNPDSFGRISIFPKELWSSAITPELKSV
                     FNKLWIEDKYHSRLEKRFNLMLVAALLTQKDNNLIHQLSALFEFHYQQAPQQLGVIYQ
                     YYFEQAGVCH"
     gene            688..7002
                     /locus_tag="RHW33_002920"
     CDS             688..7002
                     /locus_tag="RHW33_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1646403.1"
                     /translation="MDVQQLHYAPLLNQLHQRAVRSVVSQLALRSKSLTHYLESQYFQ
                     PPGQEGSLLADPVFESTFGWMPADETMEDLRDKLISSPLVDALSKAPVPFRHQLTAWR
                     TILEDKKSLLVSSGTGSGKTECFMVPVLEDLIRQQKSTRQSLTGTQAIFLYPLNALIN
                     SQQERLREWTRGFKGKIRFALYNGETRHTKYEIQEDQLKVPEQALSREAIYEAPPSIM
                     VTNTTMLEYMLIRRKDAPIIEKSQGMLKYIVLDEAHSYIGSQAAELALLLRRVMQAFN
                     VGPGTDKPVQIIATSATIGEDSPEGNKELAKFVADLAGVTERDVKVVRGYRQIPRVSE
                     SLIRHEYPTSLTSLKSLSPQDLYQQLCHYRVAQQLRQALTHPGRQAVRLSELLNVARR
                     TWPDINHRELLQLLDLMARSREGELAFTPLRMHGFIRTLAGLWACSNKQCSHKAHELN
                     QSDWPFGQVWFEQRQYCDCGAPVFEVLRCSGCGSAYLSAKEEMRGDGTNWLVAQPAAA
                     EVDEFALDVDVYSEDEDNDELDNNSQFDRLIASDGEKYIISLEETTAGKIDSEAQKHY
                     EINLLRPESRGEGRNNSFACVCCGDTQRKNNPLFRPLRLGAPFFLNEIIPTLLEFSPL
                     PQTREEQLGPFNGRRLLTFTDSRQGTARISARLQQDADKATLRALCYQELANITEPSA
                     KLSPAHCALLEKVISQFSVLPLKNSIDALKKAMAANEELSPTDIQALKIIEPMVSSLS
                     TEHAEAMQILQANSGNQQKKTMSWQTLSEQLVGSVDLADRMRGSFKELSGLDLTKQQF
                     ADFCLYSEFGRRPKNAWTLESLGLVAIHYPFIDKVTTCPQDWKTLLPDPDKQLAEWRN
                     LLKITLDFFIRENSAVFYENEQYPRWMGARFPVKMLQGPDKKNKGQKRDQLWPQIRDR
                     HYNRVIKLLIAVFPAIQPEQAHWKSLVNHLMIEVWDAIRPCLRQFESGYQLDIKQQAE
                     FYSPKQVWRCPYTRRALDVTLLGYSPYLPGSKEIAPEKAVLIEMPELPVRHWRLSGGG
                     EIAREERLEWLESNALIQHAREEGLWSTRSDRLALKDSWYRLEEHSAQRTPEQNQFNE
                     KQFKSGKVNVLNCSTTMEMGVDIGGMSLVAMNNVPPAPANYLQRAGRAGRRGESASAA
                     ITLCKNTAHGMEVFKDPLWAFNTTASAPRVRFGSSSIVQRHVNALVLGLFLRAEVPDA
                     TKLSCKWFFEGDESQCLRFLHWLNHQADELADKLKRLTQGTVLMSLTATQLLTRTQAM
                     MQQVDIRWRSQLAILLENIEALKADNSAWEETPAGKAIAYQLRDYRGAYLFSKLISEA
                     FLPGHGFPVGVVNFNYLTADELEKRRAIKATQADPNEGGESFSRRIEKLPSRDLPTAL
                     REYAPGADVVLGGKVYRSSGIMLGKVLASGQELSGDHHIPWFWHCRKCGAGATSTTRP
                     VECSHCKADIQQLDVKRYLQPVGFATDIRYQAHNDVSMPAQLPWKDPRVLVPSSVWVS
                     LPDAGLGRYRFSHSGELFHFSEGEFGHGYAICLSCGRAESQTQPQRTPENLKNPEREN
                     THYLLRGGSNDRQGSNKLCHGHVHKDLWLGYSSRTDMVELQLNDDNGLLIRDEVAARS
                     LAVALREGLAHKLGIENTELGVTTQQARDINGYTGYSIFIYDNNAGGAGYAVQLIDHW
                     ADVFNYARKLLDCSCDKFCHHCLLSYDSQHYVNRLDRHHALTLLTNVRLQRLNLAPEY
                     QYFGDGSRVETNPLSLRIAQCLNSEIYDSCSLVLAGPQEQWDFAQWPLFKELLQFASS
                     GGNVELLVATPLANLTDSSRHQLSALAAMPGGRLQVKSIATAQLMQGKGRWLAQVTRE
                     GQSQQWAADDSATVAPGELWGQSASSPVVTLKGTSGKTFSGQTLSAEDLLPALPTGAV
                     RINLCEQLDGPLEGFGSRFWSLVTQQHAGWKQAFTRHKEITHVEYSDRYLNSPFTARL
                     LGEILTELVEQGMAERASLTVCVKKLDYNSRQHDALYNAWLNEEDRQQVVTTLLEEGY
                     LGPAWPGAISWLTGDNQSTEHGRELTVTFSDGSQHYVLLDMGLSYWRCIEDTFFDFAL
                     RVPQQVERLANTRARAVAPGNDLRSYIIAG"
     gene            7226..10084
                     /locus_tag="RHW33_002921"
     CDS             7226..10084
                     /locus_tag="RHW33_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1646404.1"
                     /translation="MNKQNYAPGMRVVIRDAEWRIRRADDSGDGGYLLTCDGISELVR
                     GKEGLFLTKLEQKVEILDPAKTHLVEDESANYQAAQLYIESQLRQRVPTDSKVHFGHL
                     AAMDSMPFQLDPTRMALAQPRQRILIADAVGLGKTLEAGILVSELIRRGRGKRILVLA
                     VKSMLTQFQKEFWSRFAIPLTRLDSAGLQKVRNRIPTNHNPFHYFDKTIISIDTLKQD
                     IEYRHHLENAWWDIIVIDEAHNVAERGTSSLRSKLAKLLAGRSDTLIMLSATPHDGKA
                     ESFASLMNMLDPTAIANPKEYEYADFADKNLVVRRFKKDVKDQMSGEFPERNIVKLTR
                     LASGAEEEAYRRLVESQFRDDDDEQAQSNKGRLFKITLEKALFSSPMACASVVANRLK
                     RLESRKDHNSQSQINELESLLLALNNIDASQFSKYQLLLDTIRKDLAWKANNTEDRLV
                     IFTESIKTLEFLEQQLRADLKLKDDQIATLRGDQGDTVLMETVEAFGKTQSPLRLLVC
                     SDVASEGINLHHLSHKMIHFDIPWSLMVFQQRNGRIDRYGQKHQPQIRYLLTEASEPQ
                     INGDMRVLEVLINKDEQAQKNIGDSSEFTGKFTQEEEEEQVAEFMMQDDGASLFDQLL
                     NSNVSESAEHDLFGEICSAVSSDASMVTEADTSLFASEQAYCERALGYLKASGQTIQY
                     ETLPDNTLSLVAPEELRRRFNQLPPEIAPENWQLYLSQDKTVITDAIARARGEQHAWP
                     DVQYLWQINPVVQWLDDKISSAFGRHQAPVIRLPYLLEPDEDHFILSGLFPNRKSHPM
                     VNPWIVVSFNRESLIGSQPFAEFLQRHPQLSNKLTNSGGKDRNHQRQQDLLEAAIAHA
                     REVFIHDRNAFETHINQQLNEHLQKLDVLRGRQLSQLELDFADNKQQLSVKQSRKEQR
                     QREIEHNFDSYIEWIEDTMTTEKEPYIQVIAVITGAEG"
     gene            10087..14991
                     /locus_tag="RHW33_002922"
     CDS             10087..14991
                     /locus_tag="RHW33_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDV1646405.1"
                     /translation="MALVGINNENEFYSNHYLGEVFTSDIRDVLEPWIAQENAAREAE
                     RAAREQGKDVEPGYRAPWNQFNSLATEFFRKLAEHEKQRQIPQRLADQRNRWQPLLKA
                     LGYEITPQIQMLDDDTPLPVLARYNSTDGSPWLWIVEAHDQEEGTLDPLALSLLTAQF
                     PADTDKHKRDSLRKKANGEYRSWQDLLSTAVFTQNEPPRFVLLLGNRQLLLLDRTKWA
                     QNRLLRFDFEEILSRRETDTLKATAVLLHKDSLLPGSGAPYLDSLDDNSHKHAFGVSE
                     DLKYALRESIELLGNEAMHYLIDRGLANYTGNRAVDPDELSRECLRYMYRLLFLFYIE
                     ARPELGYAPMTAKTYLQGYSLETLRDLEMIPLTSEEDRNGRYFHDSLNMLFKLVREGY
                     NGGVKMQSDLESGDRITIHSHQFSVPRLESHLFDANNTRILNRVVFRNETLQQIIQAM
                     SLSRPAKGRFNRRGRISYRQLGINQLGAVYEALLSYRGFFASEDLYEVKKAGEEFNEL
                     ETGYFVSKDEIGKYHEDEKVYEKDGSLRIHRKGSFIYRMAGRDREKSASYYTPEVLTR
                     SLVKYALKELFKEQIDPISDPHAKADAILNLTVCEPAMGSAAFLNEAINQLAEAYLFH
                     KQQAEGRRIPQDRYTQELQRVKMYIADNNVFGVDLNPVAVELAEVSLWLNAISGDAFV
                     PWFGYQLHCGNSLVGARRQVFNKSELTYKKAKDPSWLNSEPVELAMNTPREETQIFHF
                     LLPDGGMANYSDKTVKQRYPDDFKALDSWRKEFIKSFAGHEIADVQRISEKVEALWNT
                     YRQQLKAERLKTADSYPVWPAENSEQTRSSLSSKDETFSGRLEDNSAYQKLRWVMDYW
                     CALWFWPIDKADELPDRGTWLFEIETLLDGIVITEKVTEVAEHTTGDLFAEEGLLREE
                     SSLFSGAGRLKTEVLFRHLPRLAIVDALRKQHRFFHWDLEFCDLFAERGGFDLMLGNP
                     PWLKVEWQEAGVLGDYEPEFVLRKLSASKLATLRVDTFNQIPALEAAWRSEYEGCEGM
                     QNFLNAQQNYPVLRGVQTNLYKCFLPQAWRLGAEKGVAGFLHPEGIYDDPKGGQLRAA
                     VYPRLRAHFQFQNQRILFPIGDRVKYSINIYSSTKNDHVEFVNIANLFSAKTIELCFA
                     HDGSGEVGGIKDDETGSWNEAGHQSRILTVGMERLALFATLYDEEGTPALEARLPALH
                     AEPLVSVLEKFAKQPKRLGDLQGQYLSLEMWHETNQQSDGTIERHTQFPEDASQWILS
                     GPHFYVGTPLYKTPRAICTEKGHYDCLDLITLPDDYLPRTNYIPACDAQEYAKRTPRV
                     SWKEQDEDEPRKVTDYYRFVNRRMFGGSSERSFISAILPKGFANINTCVATAFESTNV
                     LVNFTGLSHSIVYDFILKSTGKSDLYGAQLGGFVYTESFGVKIRTLLLNALSSEYSEL
                     WQSCYTPDFNTQRWSRDLPQLPQDFFAKLTPEWQRNCALRSDYSRRQALVEIDVLVAQ
                     ALGLTLEELLTIYRVQFPVMRQYEADTWYDQNGRIIFTPSKGLVGVGLPRTARKADLK
                     NGFVFNVDSPEWTGGDCTDQAIGWDDVKHLKTGTVSVTFDDYTRSDEGERRTVTWQAP
                     FIKPDREDDYKVAWAFFAQDKESA"
     gene            14991..21332
                     /locus_tag="RHW33_002923"
     CDS             14991..21332
                     /locus_tag="RHW33_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV1646406.1"
                     /translation="MLPSVVSRQVADSVAAFLRAAFPLNSPLFNGENNDNVSMLEQFL
                     AQPETLLKGPYLSAQLPFRKSDLPLNFFPNLTLPFPPHAHQARAFQRLGSDAPQPTLV
                     ATGTGSGKTECFMFPLLNHCAGASQAGVKAIIIYPMNALATDQASRFAKTIASDPQLH
                     GKVTVGLFVGDSEIEPSKKMSAEKVITCKHTLRESPPDILLTNYKMLDYLLMRPGDQP
                     LWRYNQPGSLRYLVVDELHTFDGAQGSDLACLVRRLKHHIGVDNQRFACVGTSATVGD
                     ELGQLLDYAKTIFDQPFTDDAVIREDRYTAAEYLQDYAIAYSQYPGPEVASALDPQGY
                     ATPVAYLNGQIPLWFPDSDLQLPDDLESDDGREKRIALGSLLRRHSVMHVLLHDLQGG
                     ILSERECLENLQVMLAESADHARRVLQSILALIAQARLEVPETEHDRQKRLQAKKARP
                     VLPFVQLRSQLWLREMRRLVASVAKTPRLVFADDVAVEDREHYLPVIHCRDCHATGWA
                     SLRHGQSVQLETDLDGIYRQFFEKGRSIVLAFPDNNEKAVSGAHQKLCPTCLKLNRQS
                     SVQCGHCGHTELLQVLIPDMLKERKDELEFANECPYCNSKQGISILGSRAASLSAVMI
                     HQLYGSQFNEHKKLITFSDSVQDAAHRASFFTARTWPLMIRGQIASVLGEKETLPLDA
                     FAARVCQQTRERSPDDAHFAANFIAPNMQWLNDYQTLKNEGKLAENSDLPELVSLRLD
                     WEVYSEFTLRARIGRTLEATGYAVAGVDKERFNPAITALHQQLCEELGDEMSGVSVEQ
                     VAHLALGVIWYQRQAGAVMHPAFESYRRDGTTFMMTQKERTSRYMPSIGPKTRKPAYA
                     SFEKINGFHRLIGAKSNPSWYQHWINRTLSNGNNLFISSVAETVLRRLFTALKIAGVV
                     KDFDTKGREAWGLVPSALVVSRDVVQLNCSCCREVVRAPADQGWQWRNAPCLSLRCEG
                     RYQEVDNTATWRWENMDIARVQGAEHTGLLSREDREATEKSFYRGNQPWNINLLSATP
                     TLEMGIDVGDLSTVLLCSVPPAQANYLQRIGRAGRKDGNALNITVAEGNPHDQFFFEE
                     PLEMMQGQVQAPGVFLNATAILERQLAAFCMDNWVKTGVPESAICKNVKQMLDELEFG
                     RKSGFPYNLLRYIEQHHAEIAAQFTAIFPDLAAETRQQLLSYLQGAPGQRSLVQRIEE
                     ALKLLVEDRKSLRSRIDKLKRSIDKLENAPRDQNFDSDMRELTSERQALMALVNQINN
                     KQTLNFLTDEGLLPNYAFPEAGITLRSVLWRRKEGGEAREYQNTTFEYERPASTALAE
                     LAPLNNFYAGGHKVEIEQIDLKVSEPENWRICSHCNYSENIDQTGDQHKYCPKCGTPG
                     WADAGQKTTLLKLRQVYARASARDSQISDESDSREPAFFQRQLLVSFEKEDVSAAYAI
                     EEGEVPFGFEFLSKVTLRDINFGKMADDANELMIAGEAKKRTGFKVCLGCGMVQRPRD
                     HEPRHDLSCKYRTEPEKAKFEDYLYLYRQLESEALRILLPVSSYSNDRVVEASLGAAI
                     QLGLKHYFKGNVDHLKGVVYREPENEGESWRQYLVIYDTVPGGTGSLKELMRTPDNLL
                     KLLELAYQAIVECSCNNDTHKDGCYRCVYAYRDRGRMKYVSRDQARLLLAKILQARDS
                     IRVIDSIKNISLEAMMGSELEKRFISCLQENKNLIVSRSYAHQGAGWIINTRSEPMMS
                     WHLKAQVDLGAKEAVGIPCRPDYVLYPLMQSDAIKPVAIFLDGFAFHKNSVAEDVQKR
                     QAIRDSGNFWVWTVTWADLKEPGLKHVQDVLGLGHNPDMKQPKFYNLFHDTNFAALEA
                     SFRERNSFALLLDYLADPGGRTQLWQRMAAAHAWVWLDVKKSQDAGTKQKYAYEMQEN
                     APAWRLAELLPDEPFVFGGLLDSCNSSQQFIELASVLPQQAIKPTTSVAQMRSWLRLH
                     ICFDDRYTQDDGYEAGLNGFWRLVNLLQFLPDMTFTSRKAVHLPQESVDTKVSATPEA
                     VPETDESWAEIIEFGLLSAEEIALLQSHSLSAPTLGYELQNDEGEIIAEADLAWPSQK
                     WALIIDNQEFIPLFTTRGWHVAFGPIDENTLQHLSGGDK"
     gene            21329..23443
                     /locus_tag="RHW33_002924"
     CDS             21329..23443
                     /locus_tag="RHW33_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV1646407.1"
                     /translation="MTVQTPKVALSDGFLGAFARIPKAQQKKVQEFISKFRQDPTSNG
                     LNYEKIHDARSKNVHSVRIDQTYRGIVLKPEQGALYMLMWVDKHDEAYDWARRHDCSI
                     HPVTGAIQVIDISYIKPAPEAVVDKPKLFAAYSAEQILALGVPPVFIDQVMALTDEAG
                     LNQLESIMPAEAWEPLHWLAEGLDYQEVLEEFNDHRDEPVDTNDFMEAIERSKRRFHV
                     VENEQELLQMLNAPLEKWRVFLHPSQRKLVESPANGPVRVLGGAGTGKTVVAMHRARW
                     LSQRLADKPGKKVLFTTFTRNLAADIRANLQRLCTREEMARIDVVNIDAWMSDQLKRH
                     GYDFRVVYDSDEGRRKCWNYALQQAPAELGLPDNFYAEEWQRVVQPNAVYTREEYLKV
                     SRVGRGTALSRIQRAKIWPVFEEFRAQMARAKLREMGDAMHEAIVLFKEKQVQLPYSS
                     IVVDEAQDIGAPAFTLIRSLVPESPNDLFIVGDGHQRIYRNKVVLGQCGINVRGRRSK
                     KLKINYRTTEETRQFAVGLLTGVKVDNLDGEADTSNDYLSLLHGEKPMITHAADFKEE
                     AATLVKQIQALLANQVRSQDICITARTKHACDRYASALNDAGIETFNLGNDSGDSDAR
                     PGVRVATMHRIKGLEFQYVFLVGINEGVVPEIKALASDDPVEQRDALFNERALLHVAA
                     TRAVKGLFVSSSGVPSPLLVAD"
BASE COUNT         5991 a         5641 c         6366 g         5717 t
ORIGIN      
        1 atccttgctg gatctggact tgttcagagg tatttcgagt aattaccgcg tggttgttca
       61 attgttaata tcatcatgtc tttctggtga tagcgatacg atttatgata tacaggaaga
      121 attacccttt tcatggggat ggattcccgt ttcaatctgg aaagatgttt tccaaaaatg
      181 ttggacttat ctggagaaac agattaacga taaaacatta gcattacata tattgcaacc
      241 ctttattgct tttatgaacc atcgtgcaca tatcgatcgt cggctggctc cgattgcgaa
      301 tatgttactt acatatagtg agagcctacc aaccggttgt gatgtattgc caactgttag
      361 tcgtgagcag tttaatgaag ctaaacagat gctattaagg aaccccgaca gctttgggcg
      421 tatcagtatc ttccctaaag aactttggtc tagtgctatt actccagagt taaaatctgt
      481 ttttaataag ctttggattg aagataaata tcactcacgg cttgaaaaac gttttaattt
      541 gatgttagtc gcagcgctgt taacccaaaa agataataac ctgatacatc aactgtctgc
      601 gctttttgaa tttcactatc agcaagcccc acagcaatta ggtgtaatct atcaatatta
      661 ttttgaacaa gcaggagtat gtcattgatg gacgttcagc aactgcatta tgcgccactt
      721 ctaaatcagc ttcatcagcg tgcggtgagg tcggtggtaa gtcagttggc attgcggagc
      781 aagtcgttga ctcattatct ggaatcacaa tattttcagc ctcccggtca ggaggggtca
      841 ctgcttgcag acccggtatt cgaaagtaca ttcggctgga tgcctgcgga tgaaacaatg
      901 gaggatttgc gggacaagct gatctccagc ccgctggttg atgcattgag caaagcgcca
      961 gtgccatttc gtcatcaact tactgcgtgg cgaacgattc tggaagacaa aaaatccttg
     1021 ttagtgagca gcggtacggg atccgggaaa acagaatgct ttatggtacc ggttctggaa
     1081 gatcttattc gtcagcaaaa atcgacccgc cagtcactaa cagggacaca agctattttc
     1141 ttgtatccgt taaatgcatt aattaacagc caacaggagc ggcttcgcga atggacgcgc
     1201 ggatttaagg ggaaaatacg cttcgcgcta tataatggcg aaacccggca taccaaatac
     1261 gaaattcagg aagatcagtt gaaagtacct gaacaagctt tgagtcggga agccatttat
     1321 gaagcaccac cttccatcat ggtgacgaat accaccatgc tggaatatat gctgattcgt
     1381 cgtaaagatg ccccaatcat cgaaaagtct caggggatgt taaagtatat cgtcctggat
     1441 gaagcccata gttatattgg ttctcaggct gctgagttag cgctgctttt acgtcgtgtt
     1501 atgcaggctt tcaatgttgg acccggcaca gataaacctg tgcagattat cgcgacctct
     1561 gccactattg gtgaagactc tcctgaaggt aataaggagc ttgctaaatt tgtcgctgat
     1621 ctcgcgggcg ttacagaacg tgatgtcaaa gtggttcgcg gttatcgaca aataccgcgg
     1681 gtatcggagt cgctgattcg tcatgaatac cccacatctt taacttctct taaatccctg
     1741 agccctcagg atctttacca acagctttgt cattaccgtg tggcccagca attgcgtcag
     1801 gcgttaactc atcccggacg gcaggcagta cgtttgagtg agttgctcaa tgttgccaga
     1861 agaacctggc cagatattaa tcaccgtgag ctactgcaac tcctcgatct aatggcaaga
     1921 tcgcgagaag gtgaactggc ttttacgcca ttacgtatgc atgggtttat cagaacactg
     1981 gcgggtctat gggcatgttc aaacaaacaa tgctcacata aagcacatga attaaatcag
     2041 agtgactggc catttggtca ggtatggttt gagcagcgcc aatattgtga ctgtggagcg
     2101 ccggtatttg aagttttacg ttgctccggg tgtggttcag cttatttgtc tgccaaagag
     2161 gagatgcgcg gtgatggaac gaactggttg gtagcgcaac cagccgctgc cgaagtggat
     2221 gagtttgctc ttgatgtaga tgtttattct gaagatgaag acaacgacga attagacaat
     2281 aactctcaat ttgatcgtct gattgcgagc gatggtgaaa aatatatcat ttcactggaa
     2341 gaaacgacgg cggggaaaat tgatagtgaa gcgcaaaagc actatgaaat aaacctgtta
     2401 agaccggaat ctcgaggtga gggacgcaat aactcctttg cttgtgtttg ctgtggtgac
     2461 actcaacgga aaaataatcc tttattccgt ccgcttcggc tcggcgcgcc ctttttttta
     2521 aatgaaatta ttcctactct gctggagttt tctccattac cgcaaacaag agaagagcag
     2581 ttaggaccgt ttaatggtcg acgcttgctc acctttacgg attcgcgtca gggtaccgct
     2641 cgcatttcag cccgcttaca gcaggatgcc gataaagcga cgttgcgagc tctttgttac
     2701 caggagctgg caaatattac agagccttca gcaaaactca gcccggcaca ctgtgcactg
     2761 ttagagaaag ttattagcca gttttctgtc ctccctttaa aaaattccat cgacgcgctg
     2821 aaaaaagcga tggctgcgaa tgaggagctt agcccgacgg acattcaggc acttaaaatt
     2881 attgagccga tggtgagttc cttaagcacg gagcatgctg aagcgatgca aatacttcag
     2941 gctaactctg gcaatcagca gaagaaaacc atgtcatggc aaacgctgtc tgaacagctg
     3001 gttggcagtg tggaccttgc cgatagaatg agaggcagtt ttaaagaatt gtctgggctt
     3061 gatcttacta aacaacagtt tgcagatttt tgtttataca gcgagtttgg tcgccgccct
     3121 aaaaatgcct ggaccctcga aagtctgggt ctggtagcca ttcactatcc attcattgat
     3181 aaagttacaa cctgcccgca ggactggaag acattattac ccgatcccga taagcaatta
     3241 gcggaatggc gtaatttatt gaagattact cttgatttct ttatccgtga aaacagtgcc
     3301 gttttttacg aaaacgagca ataccctcgc tggatggggg cacgtttccc ggtaaaaatg
     3361 ctccaggggc cagataaaaa aaacaaaggg cagaaacgtg atcaactttg gccacaaata
     3421 cgcgatcgcc attataaccg ggtcattaag ctattaatcg cagtctttcc ggcaatacaa
     3481 cctgagcagg ctcactggaa atcgttagtt aaccacttaa tgattgaggt ctgggatgca
     3541 attcgaccct gcttacgtca gtttgagtcg ggttatcagc tggatattaa acaacaagct
     3601 gaattttaca gtccaaagca agtgtggcgt tgcccctata ctcgacgtgc gttagacgtc
     3661 acgttgctgg gatactctcc gtatttgccg ggtagcaaag aaattgcacc tgaaaaagca
     3721 gtgttgattg aaatgcctga gcttcctgta cgtcactggc gtttgtctgg tggtggagaa
     3781 atcgcccgag aagaacgtct ggagtggctg gaaagtaatg cgctgattca acatgcccgc
     3841 gaggaaggat tatggtctac tcgcagcgac cgactggccc tgaaagatag ctggtatcgt
     3901 ctggaggaac actctgcgca gagaacgcct gagcagaacc agtttaatga aaaacaattt
     3961 aagtcaggca aagttaatgt gctgaactgt tccacgacca tggagatggg ggtggatatt
     4021 ggtggtatga gtttggtggc aatgaacaac gttcctccag cgccggcaaa ttatttgcaa
     4081 cgtgcaggtc gcgcggggcg tcgtggagag agtgcatctg ctgccattac gttatgtaaa
     4141 aacacggcgc atggaatgga ggtattcaaa gaccccctct gggcgttcaa cactactgcc
     4201 agtgcgccac gggttcgttt tgggagcagt agcattgttc aacgacatgt taatgccctg
     4261 gttttaggtc tgtttttacg agctgaagtt cctgatgcga caaaactgag ttgcaaatgg
     4321 ttctttgaag gtgatgagag ccagtgtctt cgttttcttc attggttaaa tcaccaggcc
     4381 gatgagctgg cggataaact taaacgcctt actcagggaa ccgtcctgat gtcattgacg
     4441 gcaacccagc tcttgacccg cacacaagca atgatgcagc aggttgatat ccgctggaga
     4501 agccaacttg cgatactgtt ggaaaatatc gaagcgttga aggcggataa cagcgcgtgg
     4561 gaagaaacgc cagcaggtaa agccatcgct tatcagcttc gtgattatcg cggtgcatat
     4621 ctgttttcta aactcattag tgaagcgttc ctgccggggc atggtttccc ggttggcgtc
     4681 gtcaatttca attatctgac ggcagatgag ctggaaaaac gtcgcgcaat aaaagctact
     4741 caggctgacc ctaatgaagg gggagaaagc ttttctcgtc gtattgaaaa attgcccagc
     4801 cgtgatctac ctacagcatt acgcgagtat gcgcctggcg cagatgtggt attgggcgga
     4861 aaagtatatc gttcctcggg cattatgctt ggcaaggtac ttgccagcgg gcaggagctg
     4921 tcaggcgatc atcatattcc gtggttctgg cattgccgta aatgtggtgc gggggcaacc
     4981 agtactaccc gtcctgtcga atgtagtcac tgcaaagcgg atatacagca acttgatgtg
     5041 aagcgttatt tacagcctgt tggttttgcg acagatattc gttatcaggc acataatgat
     5101 gtatccatgc ctgcacaact tccctggaaa gatccacgtg ttcttgtacc gtcttcggtg
     5161 tgggtttctt tacccgatgc tgggctagga cgttaccgtt ttagccatag tggggaattg
     5221 ttccacttca gtgaagggga atttgggcac ggctacgcaa tttgcctctc atgtggtcgg
     5281 gcagaatctc aaactcaacc ccagcgtacc cctgaaaatc taaagaaccc agagagggaa
     5341 aatacgcatt acctgttgcg agggggcagt aacgatcgtc aggggagcaa taaattgtgc
     5401 cacggtcatg tgcataaaga tctgtggttg ggatatagca gtcgtacaga tatggttgag
     5461 ctgcaactca atgatgataa tggcctgttg atccgtgatg aggttgccgc gcgttccctt
     5521 gcggtcgctt tgcgtgaagg gttagcgcat aaattgggta tcgaaaatac tgagctgggc
     5581 gtgacgacgc agcaagcgcg cgatatcaat ggctataccg gatattccat cttcatttac
     5641 gacaataatg cgggtggtgc aggttatgca gtgcaattga tcgaccattg ggctgatgtc
     5701 tttaattacg ccagaaaatt gctcgattgc agttgcgata aattctgtca ccactgcttg
     5761 ctcagctacg acagtcagca ttatgtcaac agattagatc gtcaccatgc attaaccctg
     5821 cttaccaatg tacggttgca gcgacttaac ctcgcgccag aatatcagta cttcggcgat
     5881 ggtagccggg ttgaaactaa ccctctgagt ttacgtattg cgcaatgcct gaacagcgag
     5941 atttatgact cctgctcgct ggttcttgcg gggccgcagg aacagtggga ctttgcccaa
     6001 tggccattat ttaaagagtt gctccagttt gcctcctccg gtgggaatgt tgagttgctg
     6061 gtggccacgc cgctggcaaa cttaactgat agctccagac atcagctttc cgcgttggcg
     6121 gcgatgccgg gaggtcgctt acaagtgaaa tccattgcca ctgcgcagct catgcaagga
     6181 aaggggcgct ggcttgcgca ggtgactcgc gaagggcaaa gccaacagtg ggccgcagac
     6241 gacagcgcta ccgttgcacc tggtgaatta tggggacaaa gtgcgtcttc accagtagtg
     6301 acacttaaag gaacgtccgg aaaaaccttc tcgggtcaga ccttaagtgc agaagattta
     6361 ctgcccgcct tacccaccgg ggcggtgcgc atcaatctct gtgaacaact ggacgggccg
     6421 ctggaaggtt ttggttcgcg tttctggtcg ttagtcacgc aacagcacgc tggctggaag
     6481 caggcattta cccgccacaa agagataacg catgttgagt acagcgatcg ttatcttaat
     6541 agcccattca cggcgcgttt gttaggtgaa atcttaaccg agctggttga acagggaatg
     6601 gcggaacgcg ccagcctcac tgtttgcgtc aaaaaattgg attacaactc tcgccaacac
     6661 gacgctctct ataacgcgtg gcttaatgag gaagatcgtc agcaggttgt gaccacttta
     6721 cttgaagaag gatatctggg gcctgcgtgg ccgggagcaa ttagctggtt aacgggggat
     6781 aaccagagta cggagcatgg acgagaactg acagtcacct tcagcgatgg cagtcagcat
     6841 tatgtcctgc tcgacatggg gcttagctac tggcgctgta ttgaagatac cttctttgat
     6901 tttgccctgc gagtaccgca gcaagttgaa cggctggcaa acacgcgagc gcgagctgtt
     6961 gccccgggta atgaccttag gagttatatt atcgctggat aaataattgc tcccctgggg
     7021 atatcttcca ggggagttga cccagctcac tttttaataa tttttgtttg aaaatcatgc
     7081 gcaatttatg cgcattccct cccattttgt cactttgccg ggggccttca tgatatcttt
     7141 aaggccctct tttttatcgc agtgtgccgc gccacatttt tcaggatgac gttaccgcca
     7201 ctgccccaaa tagacagaaa aaccgatgaa taagcaaaat tatgcacctg gtatgcgggt
     7261 ggttattcgt gatgccgaat ggcgtattcg ccgggcggat gacagtggtg atggtgggta
     7321 tttgctgacc tgcgatggta tttcagagct tgtgcgcggt aaagaagggt tatttctgac
     7381 caaactggaa caaaaagtag agatcctgga tcccgcaaaa acgcatttgg tggaagatga
     7441 atccgccaat tatcaagcgg cgcagttgta tattgaaagt cagttacgcc aacgcgtacc
     7501 gacagacagt aaagtccatt ttggtcatct ggcggcgatg gattccatgc cgtttcagct
     7561 cgatcctacg cggatggcgc tggcacagcc gcgccagcgg atactcattg ccgacgctgt
     7621 cggtctgggt aaaacgctgg aagcgggtat tctggtttca gaactgatcc gtcgtgggcg
     7681 cggaaaacgt attctggttc tggcggttaa atcgatgctg acacaattcc agaaggagtt
     7741 ctggagccgt tttgcgattc cattgacccg cctggattct gctggtttac aaaaggtgcg
     7801 taaccgcatc ccaaccaacc acaacccgtt ccactatttt gacaagacca ttatctccat
     7861 cgatacgctg aaacaagaca ttgaatatcg tcaccatctg gaaaatgcct ggtgggatat
     7921 tattgtcatt gatgaggccc ataacgtcgc agaacgtgga accagttcgt tacgcagtaa
     7981 actggcaaaa ctgctcgccg ggcgtagcga tacgctgatt atgctttcag caacgccgca
     8041 tgatggtaag gctgaaagtt ttgccagcct gatgaacatg cttgacccga cggcgatcgc
     8101 caacccgaaa gagtatgaat acgccgactt tgccgataaa aatctggttg tccgtcgctt
     8161 caaaaaagac gtgaaagatc agatgtccgg cgagttcccc gagcgcaata ttgtcaaatt
     8221 gactcgcctg gcatccggtg ccgaagaaga agcataccgt cgtctggttg agagccagtt
     8281 ccgtgatgat gacgatgaac aggcccagtc taacaaaggt cgtctgttca agataaccct
     8341 cgaaaaagcg ctattttcca gcccgatggc gtgtgccagc gtcgtggcga atcgtctgaa
     8401 gcgtcttgag agccgtaaag atcataatag ccagagccag atcaacgaac tggagtcatt
     8461 gctgttggcg ctgaataaca ttgatgccag ccagtttagc aaataccagt tgctgctcga
     8521 caccatccga aaagacctcg cctggaaagc caataacacg gaagatcgcc tggtgatctt
     8581 caccgaaagt attaaaacgc tggagttcct cgaacagcaa ctgcgagcgg atctgaaatt
     8641 gaaagatgac cagatcgcca cgctgcgcgg cgatcagggc gatacggtat tgatggaaac
     8701 cgtagaagcc tttggtaaaa cgcagtctcc gttgcgtctt ctggtttgtt cggatgtggc
     8761 atctgaaggg atcaacttgc atcaccttag ccacaaaatg attcactttg atattccgtg
     8821 gtcgttgatg gtattccagc aacgtaacgg gcgtattgac cgctatgggc aaaaacatca
     8881 gcctcaaatc cgctatctgc ttactgaggc cagcgaacca cagatcaatg gcgatatgcg
     8941 cgtactggaa gtgttgatta acaaagacga acaggcgcag aagaacattg gggacagttc
     9001 ggagtttacc ggcaaattta cccaggaaga ggaagaagag caggttgcag agttcatgat
     9061 gcaggatgat ggtgccagcc tgtttgatca actgctgaac agcaacgtct cggaaagcgc
     9121 cgaacacgat ctgtttggcg aaatatgcag tgcggtttct agcgatgcct caatggtcac
     9181 cgaagcagac accagcttgt ttgccagcga acaggcgtac tgcgaaagag cattgggtta
     9241 cctgaaagcc agtggtcaaa ctatccagta tgaaaccttg cctgataata ccttgtcgct
     9301 ggtggcaccg gaggagttac gccgccgctt caaccagcta ccgcctgaaa ttgccccgga
     9361 gaactggcag ctctatttaa gtcaggataa aaccgtcatc accgacgcga ttgcacgcgc
     9421 tcgcggtgag caacatgcct ggcccgatgt gcagtatctc tggcaaatca acccggtagt
     9481 gcagtggctg gacgataaaa tctcttcggc ttttggtcgt catcaggctc cggttatccg
     9541 gctgccatac ctgcttgaac ccgatgaaga tcacttcatt ctttccgggt tattcccgaa
     9601 ccgtaaatca catccgatgg tgaacccgtg gatagtggtg agctttaacc gtgaatcgct
     9661 aatcggcagt cagcctttcg ctgagttttt acaacgtcat ccgcagttga gtaacaagct
     9721 gactaacagc ggcggtaaag atcgtaatca ccaacgccag caggatttac tggaagcggc
     9781 tattgctcat gcccgggagg tattcatcca tgatcggaat gcgtttgaaa cgcacattaa
     9841 ccagcaactg aatgagcatc tgcaaaagct ggacgttttg cgtgggcggc agttgagcca
     9901 acttgagctg gattttgccg ataacaaaca gcaattgtca gtcaagcaga gccgtaaaga
     9961 gcagagacaa cgcgaaatcg aacacaattt tgacagctat atcgaatgga ttgaggacac
    10021 catgacgact gaaaaagaac cctacattca ggtaattgct gttatcaccg gagcggaggg
    10081 ttaatcatgg cgctggtcgg tattaataac gaaaacgaat tttactctaa ccactatttg
    10141 ggtgaggttt tcaccagtga tatccgcgat gtgctggaac cctggatagc ccaggaaaat
    10201 gcagcgcgtg aagcggagcg tgccgctcgt gaacagggca aagacgtgga gccgggatac
    10261 cgcgctccgt ggaaccagtt taacagtctg gcgactgagt ttttccgcaa acttgccgag
    10321 cacgaaaaac agcgtcagat cccgcagcgt ctggccgatc aacgtaatcg ctggcagcca
    10381 ttgttaaagg cgctgggcta cgaaattacg ccacagatcc aaatgctgga tgacgataca
    10441 ccgctgccgg tactggcgcg ttacaacagc actgacggta gcccgtggct gtggattgtt
    10501 gaagcacacg atcaggaaga aggaacgctg gatccgctgg cgctctcctt actgaccgcg
    10561 caattcccgg cggataccga caaacataag cgcgacagcc tgcgcaaaaa agccaacggt
    10621 gaatatcgca gctggcagga tctgctctct acggcggtct tcacccaaaa tgaaccgccg
    10681 cgttttgtgc tgctgctcgg taaccgtcag ctcttgttgt tggaccgtac taagtgggcg
    10741 caaaaccgtc tgctacgttt tgattttgaa gagattttaa gccgtcgtga aacggatacg
    10801 ctgaaagcga ctgcggtgtt gttacataaa gattctctgc tgccgggcag tggggcacct
    10861 tatcttgact cgctggatga caattcgcac aaacatgcgt ttggtgtttc ggaagatctg
    10921 aaatatgccc tgcgcgaaag cattgagttg ctgggcaacg aagcgatgca ttatctgatc
    10981 gaccgtggcc tggcaaacta taccggtaac cgtgcggtgg acccggatga actgagccgc
    11041 gaatgtctgc gttacatgta ccgcctgctg ttcctgttct acattgaagc gcgcccggag
    11101 ctgggttatg cgccaatgac tgccaaaact tatctacagg gctacagcct ggaaacgttg
    11161 cgcgatctgg agatgatccc gctgaccagc gaagaagatc gcaacgggcg ctacttccac
    11221 gacagcctga atatgctgtt taaactggtg cgcgaaggct acaacggcgg cgtgaaaatg
    11281 cagagtgacc tggagagcgg cgaccggatc accatccata gtcatcagtt cagcgtcccg
    11341 cgtctggaaa gtcatctgtt tgatgccaac aacacgcgca ttcttaaccg cgtggtattc
    11401 cgtaacgaaa ccctgcaaca gattatccag gcgatgtcgt taagccgccc ggccaaaggg
    11461 cgctttaacc gccgcggacg tatttcttat cgccagttgg gtatcaacca gttgggtgcg
    11521 gtgtatgagg cgctgctctc ctatcgcgga ttcttcgcca gcgaagatct ctacgaggtg
    11581 aagaaagccg gggaagagtt taacgagctg gagacgggtt acttcgtcag taaggatgag
    11641 attggcaaat accacgaaga cgagaaggtc tacgagaaag acggcagtct gcgcattcac
    11701 cgcaaaggca gctttatcta ccgtatggct gggcgcgacc gtgagaaatc tgcctcttat
    11761 tacaccccgg aagtgctgac ccgctcactg gttaaatatg ccctgaaaga actgtttaag
    11821 gagcagattg atcccattag cgatccgcac gccaaagccg acgccatctt aaacctcacc
    11881 gtgtgcgaac cggcgatggg cagcgcggcg ttccttaacg aagccatcaa ccagctggcg
    11941 gaagcgtatc tgttccataa gcagcaggcg gaaggtcgcc gtattccgca ggatcgttac
    12001 acccaggagt tacagcgggt gaaaatgtac attgccgaca acaacgtttt cggcgtggac
    12061 ttaaacccgg tggcggtgga actggcggaa gtgtcgctgt ggctgaacgc cattagtggc
    12121 gatgcctttg taccgtggtt tggttaccag ctgcactgcg gtaactcact ggtgggcgcg
    12181 cgccgtcagg tgttcaacaa gagtgaactg acctacaaaa aagccaaaga tccgagctgg
    12241 cttaacagcg agccggtcga gctggcgatg aacacgccgc gtgaagagac gcagattttc
    12301 cacttcctgc tgcccgacgg cggtatggct aactacagcg ataaaactgt taagcagcgt
    12361 tatccggatg acttcaaagc gctggacagc tggcgcaaag agtttattaa aagctttgcc
    12421 gggcatgaga ttgctgatgt gcagcgtatc agcgaaaagg tggaagctct gtggaacact
    12481 tatcgccagc aacttaaagc agaacgtctg aaaaccgccg acagctaccc ggtgtggccg
    12541 gcagaaaaca gcgagcagac gcgttcttcg ctgagcagta aagatgaaac ctttagcggt
    12601 cgtcttgaag ataacagcgc ctaccagaag ctgcgttggg tgatggacta ctggtgcgcg
    12661 ctgtggttct ggccgatcga caaagccgat gagttaccgg atcgcggcac ctggttgttt
    12721 gagattgaaa ccctgctcga cgggattgta atcacggaaa aagtcactga agttgcggag
    12781 cacaccactg gcgatctgtt tgccgaagaa ggcctgctgc gagaagagtc ttcactgttt
    12841 tctggcgcag gtcgtctgaa aaccgaggtg ttgttccgtc atttgccgcg tctggcgatt
    12901 gtcgatgccc tgagaaagca gcaccgtttc ttccactggg atctggagtt ctgcgacctg
    12961 tttgccgagc gcggcggttt tgacctgatg ctcggaaacc cgccgtggct gaaagtggaa
    13021 tggcaggaag cgggcgtgct gggtgattac gagccggaat ttgtgctacg taagctgagt
    13081 gcatcgaagc tggcaacgtt gcgtgttgat acctttaacc agatcccggc actggaagcg
    13141 gcctggcgca gcgagtatga aggctgtgaa gggatgcaaa acttcctcaa tgcgcagcag
    13201 aactacccgg tactgcgcgg ggtgcagacc aacttgtata aatgcttcct gcctcaggcc
    13261 tggcgtctgg gggcagagaa aggcgtggca ggtttcctgc acccggaagg gatttatgat
    13321 gacccgaaag gcgggcagct tcgtgcggca gtgtatccga gactgagggc acattttcag
    13381 tttcagaatc agagaatatt gttccctatt ggcgatagag ttaagtacag cattaatatt
    13441 tattccagta caaaaaacga tcatgttgag tttgttaata ttgctaacct tttctcagcc
    13501 aaaacgatcg agctttgctt tgctcatgat ggttccggtg aagttggcgg cattaaagat
    13561 gacgaaacag gttcctggaa tgaagcgggg catcaaagcc gtattttgac cgttggaatg
    13621 gaacggcttg cactatttgc cactctttat gatgaagagg gaacgccggc tcttgaagct
    13681 cgactcccag cgcttcatgc tgaacctctt gtttctgtac ttgaaaaatt cgctaagcaa
    13741 cctaaacggc tgggggattt gcaagggcaa tatttgtctc ttgaaatgtg gcatgaaaca
    13801 aatcaacaaa gtgatggcac aatcgaacgg catacccaat tcccggaaga cgcatcccaa
    13861 tggattctgt ctggtccgca cttctatgtc ggaacaccgc tttataaaac gccgagagca
    13921 atttgcacag agaaaggaca ttatgactgc ctggacctga taactctgcc cgacgactat
    13981 ttgccgcgca ctaactatat tccagcatgt gatgcacagg agtatgcaaa gcgcacccca
    14041 cgggtttcgt ggaaagaaca ggatgaagac gagccgagga aggtgacgga ttattatcgt
    14101 tttgttaatc ggagaatgtt tggtggatct tctgagagat ctttcatctc cgctattctt
    14161 cccaagggat ttgcaaacat taacacatgt gtcgctacgg cctttgagtc aactaatgta
    14221 ctggtgaatt ttacgggcct ttcgcattca attgtttatg attttatttt gaaatcaact
    14281 ggaaaatcag atctgtacgg tgcacaactt ggtgggttcg tatataccga aagttttggt
    14341 gtaaaaataa ggactttatt attaaatgcc ctatcaagtg aatactccga gctctggcaa
    14401 tcctgctaca ccccagactt caacacccag cgttggagcc gcgatctccc gcagctcccc
    14461 caggatttct tcgccaaact gaccccagag tggcagcgta actgcgcttt acgctctgac
    14521 tacagtcgtc gtcaggcgct ggtggaaatc gacgtattgg tggcgcaggc gctggggtta
    14581 actctcgaag agctgcttac catttatcgc gttcagttcc cggtgatgcg ccagtacgaa
    14641 gcggatacct ggtacgatca aaacggtcgc attatcttta ccccaagcaa agggctggtg
    14701 ggcgttggct tgcctcgcac cgcgcgtaaa gctgacctga aaaacggctt tgtctttaac
    14761 gtcgacagcc cggagtggac cggcggtgac tgcaccgatc aagctatcgg ttgggatgat
    14821 gtcaaacatc ttaaaactgg taccgtcagc gtcacctttg atgactatac ccgcagcgac
    14881 gaaggcgagc gccgtaccgt cacctggcag gcaccgttta tcaagccgga tcgcgaagac
    14941 gactacaaag tggcctgggc gttctttgca caagataagg agagcgcctg atgctacctt
    15001 ccgttgtcag tcggcaggtc gccgacagcg ttgccgcctt tttacgggcg gcgttcccgc
    15061 taaacagccc gctgtttaac ggtgagaata acgataatgt ctcaatgctg gagcaatttc
    15121 tggcccagcc cgaaacgctg ctgaaaggac catacctttc cgcccagttg ccgtttcgta
    15181 aaagtgactt accgctgaat tttttcccta atctgacgct gccgttcccg ccgcacgcgc
    15241 atcaggcccg ggcatttcag cgtctgggaa gcgacgcgcc acagccgacg ctggtcgcta
    15301 ccggtaccgg ctccggtaaa acagaatgct ttatgttccc gctgctcaac cactgcgcgg
    15361 gcgcgtcaca agccggggtt aaagcgatta tcatctaccc gatgaacgcc ctggcaaccg
    15421 atcaggccag ccgtttcgcc aaaaccatcg ccagcgatcc acaactgcat ggcaaggtga
    15481 ccgtcggctt gtttgtcggc gacagcgaaa tcgaacccag caaaaaaatg tcggcagaaa
    15541 aggtcattac ctgcaagcac acgctgcgcg aaagcccgcc ggacatcctg ttgaccaact
    15601 acaaaatgct cgactacctg ctgatgcgcc ctggcgatca gccgctctgg cgttacaacc
    15661 agcccggttc attgcgttac ctggtcgtgg atgaattgca tacgtttgat ggcgcgcaag
    15721 ggtcggatct ggcctgtctg gtgcgacgtc tgaagcatca cataggtgtg gataaccaac
    15781 gattcgcctg tgtcgggaca tcagcgaccg tcggcgacga gctggggcaa ctgctcgatt
    15841 acgcaaaaac aatctttgac cagccgttta ccgatgacgc cgttattcgc gaagatcgct
    15901 acacggcagc ggagtacttg caggattacg ccattgcgta cagtcagtat ccggggccgg
    15961 aggtcgcttc ggccctggac ccgcagggtt acgctacccc ggtggcgtat ctcaacggac
    16021 aaatcccttt gtggttcccc gacagcgact tgcaactccc ggacgatctg gagagcgacg
    16081 acgggcgtga aaagcgtatt gcgctggggt cactgttacg ccgccacagc gtgatgcatg
    16141 tcttactcca tgatctgcaa ggcgggatcc tctctgagcg agagtgtctg gaaaatctcc
    16201 aggtcatgct ggcagaaagc gccgaccatg cccgacgcgt gctgcaaagc attctggcgc
    16261 tgatcgcgca ggcacgtctc gaagtaccgg aaacggaaca cgatcggcaa aaacgtctcc
    16321 aggcgaagaa agcgcgcccg gtgctgccgt ttgtgcaact gcgctcccag ttgtggcttc
    16381 gtgagatgcg tcgtctggtc gccagcgtgg cgaaaacgcc gagactggtg tttgccgatg
    16441 atgtggcggt ggaagaccgc gaacactatt tgccggttat ccactgccgt gactgccacg
    16501 caaccggctg ggccagcctg cgtcatgggc aaagtgttca gctggaaaca gacctcgacg
    16561 gcatttatcg ccagttcttt gaaaaagggc gctccatcgt ccttgctttc ccggataaca
    16621 acgaaaaagc ggtcagtggc gcacaccaga aactgtgccc tacctgtctg aaactgaacc
    16681 gacagtccag cgtccagtgt ggacattgcg gtcatacgga gctgttgcag gtactgatcc
    16741 cggatatgct caaagagcgt aaggacgagc tggagtttgc taatgaatgc ccgtactgca
    16801 acagcaaaca ggggatttcg attctggggt cacgggctgc cagtctgtcg gcggtgatga
    16861 ttcaccagct ttatggcagc cagttcaacg aacataaaaa gctgattacg ttttctgact
    16921 cggtacaaga cgccgcgcat cgtgccagct tctttactgc ccgtacctgg ccgctgatga
    16981 ttcgtgggca aatcgccagt gttcttggcg aaaaagagac attaccgctg gatgcgttcg
    17041 cggcgcgtgt ttgccaacaa acgcgtgaac gttcaccgga cgatgctcat tttgcagcga
    17101 acttcatcgc accaaatatg cagtggctga atgattatca gacactgaaa aatgaaggca
    17161 aactggcgga gaactccgat ctgccggagc tggtttctct gcgcctcgac tgggaggtgt
    17221 acagcgaatt taccctgcgc gcgcgtatcg gacgtacgct ggaagccacc ggttatgccg
    17281 ttgccggagt ggataaagaa cgctttaatc cggcaattac cgccctgcat caacagcttt
    17341 gtgaagagct gggtgatgag atgtccggtg tgagcgtcga gcaggtagcg cacctggcgc
    17401 tgggggttat ctggtatcaa cgccaggccg gggccgttat gcatccggcg ttcgaaagtt
    17461 atcgtcgtga tggcacgacc tttatgatga cgcaaaaaga gcgtacctcg cgctatatgc
    17521 cttctatcgg tccgaaaacg cgtaaaccgg cttatgcatc gtttgaaaaa ataaatggtt
    17581 tccatcgcct gattggcgcg aagtctaacc ccagttggta tcagcattgg atcaatcgca
    17641 cgctgagtaa cggcaataat ctgtttatca gtagcgttgc ggaaacggtg ttacgacgcc
    17701 tgtttacggc gttgaaaata gccggagtag taaaagattt tgacaccaaa gggcgtgaag
    17761 cctggggact ggtgccttcg gcgctggtgg tgagccgtga cgtcgttcag cttaattgct
    17821 catgttgccg tgaggttgtc cgggctccgg ccgatcaagg ctggcagtgg cgtaatgcac
    17881 cttgtctctc tttacgttgt gaaggtcgtt accaggaagt ggacaatacg gcgacctggc
    17941 gttgggagaa catggacatc gcgcgcgtgc agggcgcaga acacacgggg ctgcttagcc
    18001 gtgaagatcg cgaagccacc gaaaaatcgt tctaccgggg caaccaaccg tggaatatca
    18061 acctgttgtc ggcaacaccg acgctggaga tgggtattga cgttggggat ctttccaccg
    18121 ttttactctg ttctgtccca cctgctcagg ccaactactt gcagcgtatt ggtcgcgccg
    18181 gtcgtaaaga tggtaacgcg ttaaacatta ccgtggcaga agggaacccg cacgatcagt
    18241 ttttctttga agaaccgctg gagatgatgc agggccaggt gcaggctcct ggtgtcttcc
    18301 tcaatgcaac ggctattctc gaacgccagc tggcagcttt ttgtatggat aactgggtga
    18361 aaaccggtgt accagaatcc gctatctgta aaaacgtcaa acagatgctg gatgagctgg
    18421 agttcgggcg taaatcgggt tttccgtata acttactgcg atatatcgaa cagcatcatg
    18481 cggagattgc tgcacaattt acggcgatct tccccgatct tgccgcagaa actcgccaac
    18541 agctgttgtc ttatcttcag ggcgcgccag ggcaacgttc gctggtgcag cgtatcgaag
    18601 aggcgttaaa actgctggtg gaagatcgca aatctttgcg ctcacgcatc gataaactca
    18661 agcgcagtat cgataaactg gagaacgcgc cgcgcgatca aaactttgat agcgatatgc
    18721 gcgagttaac ttcggaacgg caggcgctga tggcgctggt caatcaaatc aacaataagc
    18781 aaacgctgaa tttcctcacg gatgaaggtc tgctgcctaa ctacgccttc ccggaagccg
    18841 gtattacgtt acgttctgtg ctctggcggc gtaaagaagg cggggaagca cgtgaatatc
    18901 aaaacaccac tttcgaatat gaacgcccgg catcaacggc gctggctgag ctggctccgc
    18961 tgaataattt ctatgccggt gggcataaag ttgaaattga gcagatagac ctgaaggtct
    19021 ctgagccgga gaactggcgt atctgtagcc actgtaacta cagcgaaaat atcgaccaga
    19081 cgggcgacca gcataaatat tgcccgaaat gtggcacacc ggggtgggcc gatgccggac
    19141 aaaaaaccac gctgctgaaa cttcgccagg tttatgcccg cgccagcgca cgtgatagcc
    19201 agatcagcga cgagagcgac agccgtgaac ctgctttctt ccagcgtcag ctattggtta
    19261 gtttcgaaaa agaagatgta tcggcggcat acgcgattga ggaaggcgaa gttccgtttg
    19321 gttttgaatt tttaagcaaa gtcaccctgc gggatatcaa cttcggcaaa atggcggatg
    19381 atgccaatga gttgatgatt gccggagaag cgaagaagcg aaccgggttt aaagtttgcc
    19441 tcgggtgcgg catggtgcag cgtcctcgcg atcatgaacc acgtcatgat ttgagctgca
    19501 aatatcgcac ggaaccggaa aaggcgaagt tcgaagatta tctctatctc tatcgtcagc
    19561 tggagtcaga agcgttgcgt attctgctcc cggtaagcag ttacagtaat gaccgtgtcg
    19621 tggaggcttc tcttggggcg gcgatccagc tcggccttaa gcattacttc aaggggaatg
    19681 tcgatcacct gaaaggcgtt gtgtaccgcg agccggaaaa tgaaggcgaa tcctggcggc
    19741 agtatctggt gatttacgat accgtcccgg ggggaactgg ttctcttaaa gagctgatgc
    19801 gcactcccga caatttgctg aaattacttg aactggccta tcaggcaata gtggaatgca
    19861 gttgcaacaa tgatacgcat aaagatggtt gttatcgttg cgtttacgct taccgtgacc
    19921 gtgggcgcat gaagtatgtc tcccgcgatc aggcgcgtct cttgctggcg aaaattttgc
    19981 aggcaagaga ttcgatacgg gtaattgatt ccatcaaaaa catttccctc gaagccatga
    20041 tgggcagtga gctggaaaaa cggtttatta gctgcctgca agaaaataaa aacctgattg
    20101 tcagtcgcag ttacgcgcat cagggcgctg ggtggattat taacacccgc agcgaaccga
    20161 tgatgagctg gcatcttaaa gctcaggtgg atttaggcgc taaagaggcg gtggggatcc
    20221 cttgtcgccc ggattacgtg ctatatccgc ttatgcagtc tgatgccatt aaaccggtgg
    20281 ctattttcct tgatggcttt gcgttccaca aaaatagcgt cgcagaggat gtgcaaaaac
    20341 gccaggcgat tcgggatagc ggtaatttct gggtgtggac agtcacgtgg gccgatctca
    20401 aagagcctgg gcttaaacat gttcaggatg tcctgggact tgggcataac ccggatatga
    20461 aacagcctaa gttttataac ctgtttcatg ataccaactt tgcggccctt gaagcgtcgt
    20521 tccgggaacg caacagtttt gcactgctgc tggattatct cgccgatccg ggcggaagaa
    20581 cgcagttatg gcagagaatg gctgctgcac atgcctgggt gtggttggat gtgaagaagt
    20641 cacaggacgc gggaacgaaa caaaagtatg cctatgaaat gcaggaaaac gcgccagcct
    20701 ggcggctagc agagcttttg ccagacgaac cgtttgtctt tggtggttta ctcgatagct
    20761 gtaactcatc gcagcaattt attgagctgg cttcggtgtt accacaacaa gccattaaac
    20821 cgacgaccag cgtcgcacaa atgcgcagct ggctgcgttt gcatatctgt tttgacgatc
    20881 gctatacgca ggatgacggt tatgaagcgg gactcaatgg cttctggcga ctggtaaacc
    20941 tgttacagtt cctgccggat atgaccttta ccagccgtaa agccgtgcat ttaccgcagg
    21001 aaagcgttga cactaaagtt tcggcaacgc cagaggcagt accagagact gatgagagtt
    21061 gggctgagat tattgagttt ggcttattaa gcgctgaaga gatcgcgttg cttcagtctc
    21121 attctctctc tgcgccgact ctcggctacg aattgcaaaa tgatgaaggt gaaattattg
    21181 cggaagccga tctcgcctgg ccatcacaaa aatgggcatt gattattgat aatcaagaat
    21241 ttattccatt attcacaacc agaggttggc acgttgcatt cgggccaatt gacgaaaata
    21301 cattgcaaca tctgtccgga ggtgacaaat gacggtgcaa acgcccaagg tggcactttc
    21361 tgatggtttt ctgggcgcat tcgcgcggat cccaaaggca cagcaaaaaa aggtgcagga
    21421 attcatctcc aaattccgtc aggacccgac cagtaatgga cttaactatg aaaagatcca
    21481 cgatgcgcgc agtaaaaatg tccattcggt gcgaatcgat caaacttatc gcggcattgt
    21541 tctgaaacct gaacagggtg ccctgtatat gctgatgtgg gtggataaac acgacgaagc
    21601 atacgactgg gcgcggcggc atgattgttc gattcacccg gttaccggcg cgattcaggt
    21661 cattgatatt agctatatca aaccggcacc ggaagcggtg gttgataaac cgaaactctt
    21721 cgcggcttac agcgcggaac agattctggc cctgggcgtg ccgcctgtct ttattgatca
    21781 ggtgatggcg cttaccgatg aagccgggct taatcaactt gaaagcataa tgccagcaga
    21841 agcctgggaa cctctgcact ggctggcaga agggctggat taccaggaag tgctggaaga
    21901 gtttaacgat catcgtgatg agcctgttga taccaacgac tttatggagg cgattgaacg
    21961 aagtaaacgt cgcttccatg tcgttgaaaa tgaacaggag ctgttgcaga tgcttaatgc
    22021 gcctttagaa aaatggcgtg tatttctgca tccgagccag cgaaaattgg tggagtcgcc
    22081 cgctaatggt ccggtacgag tactgggcgg tgcaggtaca gggaaaacgg ttgttgcgat
    22141 gcaccgtgcg cggtggttat ctcagcgtct ggcagataaa cccggtaaga aagtactttt
    22201 cacgaccttt acgcgtaacc tggcggcaga tattcgcgct aatctccagc ggttgtgtac
    22261 gcgagaagag atggcgcgta ttgacgtcgt taacattgat gcctggatga gcgaccaact
    22321 aaaacgccac ggttatgatt tccgcgtggt atacgacagc gatgaagggc gacgcaaatg
    22381 ctggaactat gccttgcagc aggcacctgc cgaactgggg ctacctgaca atttctatgc
    22441 tgaagagtgg caacgcgttg tccagccaaa tgcggtttac acccgggagg aatacctcaa
    22501 agtcagccgg gttggacgtg gtacagcgtt atcccgtatt caacgggcga aaatctggcc
    22561 agtctttgaa gaattcagag cacaaatggc gcgtgccaaa ctgcgcgaaa tgggcgatgc
    22621 catgcatgag gctattgttc tgtttaaaga aaaacaggtt caactacctt atagcagtat
    22681 tgtggtcgat gaagctcaag atatcggtgc cccggcattt actcttattc gcagcctggt
    22741 tcccgagtcg cccaacgatc tgtttattgt cggggatggg catcagcgta tttatcgcaa
    22801 taaggtcgtt cttggtcagt gtggtattaa cgtccgtggt cgtcgcagta aaaaattgaa
    22861 gattaactat cggaccacag aagagacgcg tcaatttgct gttgggctgt taactggtgt
    22921 gaaggttgat aacctcgatg gcgaagcgga taccagtaat gattatttgt cattactgca
    22981 cggggaaaag cccatgatta cgcatgcggc agatttcaaa gaagaggctg caacgctcgt
    23041 caagcaaatt caggcattgc tggcgaatca ggttcgttct caggatatct gcattactgc
    23101 gcgtactaaa catgcttgtg accgctacgc atcagcactg aatgacgccg gaattgagac
    23161 atttaattta ggcaatgata gcggtgacag cgatgcacgc cctggcgtgc gggttgcaac
    23221 gatgcaccgt attaaagggc ttgagttcca gtatgtgttc ctggtgggga ttaacgaagg
    23281 cgttgtgccg gaaataaaag cattagcatc cgatgatccc gttgaacagc gtgatgcatt
    23341 atttaatgag cgcgcgttac tgcatgtggc tgcaacccgt gccgtaaaag ggctgtttgt
    23401 ttcatcatct ggtgtcccta gtccgctatt agtggctgac tgataatatg tccaaaacag
    23461 gccaggagcc gaaacgctcc tggtctatat aaccgttctg gcaactggcg atatttatat
    23521 tgcttagctc ccaacagcca gacaatatca acttagaaat tatattcgct tatgcggttt
    23581 gcatattgca cagtacaacc tgttttctct atatctaacg taacgtcagg atgtggatga
    23641 tttttggatg gggtgacaca ctggacaaat atagctggca cgaaaaggct ggcatttcca
    23701 gctgaccaat agtct
//
LOCUS       DAPGRN010000069        23594 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPGRN010000069 DAPGRN010000000
VERSION     DAPGRN010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.069"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            59..1246
                     /gene="ompC"
                     /locus_tag="RHW33_002925"
     CDS             59..1246
                     /gene="ompC"
                     /locus_tag="RHW33_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1646408.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDLYGKVAGLHYF
                     SDDSGSDGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGF
                     GFGQNGTFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFF
                     GQVDGLNFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLG
                     AAYSNSDRTDNQVHKGTHNSRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAET
                     RNMTGYGDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGN
                     HHYTNKDLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLV
                     YQF"
     gene            complement(1247..1582)
                     /locus_tag="RHW33_002926"
     CDS             complement(1247..1582)
                     /locus_tag="RHW33_002926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646409.1"
                     /translation="MLWNIVVLMNIYIPDWWRKSLFHALFLQIFLYGKRVCYGLIHKE
                     MHLGVSLKSSWLMLLCQEGQGKVLYRYASAIACAAEGDEMSIKKPEFDEGRTQASVQF
                     YSNTPRRVG"
     gene            1838..2689
                     /gene="hchA"
                     /locus_tag="RHW33_002927"
     CDS             1838..2689
                     /gene="hchA"
                     /locus_tag="RHW33_002927"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV1646410.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2797..4155)
                     /gene="hprS"
                     /locus_tag="RHW33_002928"
     CDS             complement(2797..4155)
                     /gene="hprS"
                     /locus_tag="RHW33_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV1646411.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4155..4826)
                     /gene="hprR"
                     /locus_tag="RHW33_002929"
     CDS             complement(4155..4826)
                     /gene="hprR"
                     /locus_tag="RHW33_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV1646412.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLITTIRGMGYSF
                     VAVKK"
     gene            4959..5372
                     /gene="hiuH"
                     /locus_tag="RHW33_002930"
     CDS             4959..5372
                     /gene="hiuH"
                     /locus_tag="RHW33_002930"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV1646413.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5481..6485
                     /gene="msrP"
                     /locus_tag="RHW33_002931"
     CDS             5481..6485
                     /gene="msrP"
                     /locus_tag="RHW33_002931"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV1646414.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6486..7121
                     /gene="msrQ"
                     /locus_tag="RHW33_002932"
     CDS             6486..7121
                     /gene="msrQ"
                     /locus_tag="RHW33_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV1646415.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQQKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            7378..8028
                     /gene="zinT"
                     /locus_tag="RHW33_002933"
     CDS             7378..8028
                     /gene="zinT"
                     /locus_tag="RHW33_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV1646416.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8371..8901
                     /locus_tag="RHW33_002934"
     CDS             8371..8901
                     /locus_tag="RHW33_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV1646417.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(9471..9560)
                     /locus_tag="RHW33_002935"
     tRNA            complement(9471..9560)
                     /locus_tag="RHW33_002935"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9524..9526),aa:Ser,seq:cga)
     gene            9654..10451
                     /gene="mtfA"
                     /locus_tag="RHW33_002936"
     CDS             9654..10451
                     /gene="mtfA"
                     /locus_tag="RHW33_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV1646418.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            10552..10627
                     /locus_tag="RHW33_002937"
     tRNA            10552..10627
                     /locus_tag="RHW33_002937"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10585..10587,aa:Asn,seq:gtt)
     gene            10940..18922
                     /locus_tag="RHW33_002938"
     CDS             10940..18922
                     /locus_tag="RHW33_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV1646419.1"
                     /translation="MATKKRSGEEINDRQILCGMGIQLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AENMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNFSNREQSMVVVQAPTLSQKDSSVSLSTQTLSADSHSTATLTFIAHDAAGNPV
                     IGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNTAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLADENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVNSHDGIATATLTSLKNGDYT
                     VTASVSSGSQANQQVIFIGDQSTAALTLSVPPGEITVTDTAPQQLTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNGGKGMTDSNGTAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLNARNTNENGIAEVTLKGTVLGVYTAEATLPNGNRDTTTVNIAPDASNALVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNTNDSGVAEVTLKGTVLGVHT
                     VEATLLNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEVNNLPITFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVTLSNNGINTTNHDGYLYASF
                     TATKAGVYQVTATLDNGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     ITGGKSEQLVVNFTADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVT
                     LIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSST
                     SVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKADINSATITSLEIPE
                     GQVMVAQDVAVKAHVNDQFGNPILNESVTFSAAPPEHMTISQNIVSTDTHGIAEVTMT
                     PERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSANG
                     TPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQT
                     QTIVKVTGNSSTAHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKS
                     GSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADASKS
                     VLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYSK
                     NFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLP
                     TTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNWER
                     ITATPKSGGPNYVYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHSRSR
                     GIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAYA
                     TCYKNL"
     gene            complement(19184..20236)
                     /locus_tag="RHW33_002939"
     CDS             complement(19184..20236)
                     /locus_tag="RHW33_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDV1646420.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSIINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKSYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     DRTFSLSIAENLIEKIQSETDMPIVIVHGPKGEDKARELVNSYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            20551..21867
                     /gene="shiA"
                     /locus_tag="RHW33_002940"
     CDS             20551..21867
                     /gene="shiA"
                     /locus_tag="RHW33_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV1646421.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            21969..23423
                     /gene="amn"
                     /locus_tag="RHW33_002941"
     CDS             21969..23423
                     /gene="amn"
                     /locus_tag="RHW33_002941"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV1646422.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         6464 a         5595 c         5383 g         6152 t
ORIGIN      
        1 ttggataacg gcacttattg atatattcat gaagattata atcacaaggg aatacataat
       61 gaaaagaaaa gttctggcaa tgctggtccc ggcgttatta gttgctggcg cagcaaatgc
      121 ggctgaagtc tataataaag atggcaataa actggatttg tacggaaaag tagcgggtct
      181 gcactacttc tctgatgact ctggcagtga tggcgacatg tcatatgccc gtatcggttt
      241 caaaggtgaa acccagatcg ctgaccagtt cactggttat ggtcagtggg aatttaacat
      301 tggcgcaaac ggtcctgaaa gcgacaaggg taataccgca acgcgtctgg catttgcagg
      361 tttcggcttt ggtcagaatg gtactttcga ctatggtcgt aactacggtg tcgtatatga
      421 cgtagaagca tggaccgata tgctgccaga atttggtgga gatacctatg ctggcgctga
      481 caacttcatg aacggtcgtg ctaacagcgt agcaacctat cgtaacaatg gtttctttgg
      541 tcaagttgat ggtctgaact ttgcactcca gtatcagggt aacaacgaga aaagcggatt
      601 atttgatcaa gaaggttcag gtaacggtaa tggacgtaaa cttgctaaag agaacggcga
      661 cggttacggt atgtccactt cctatgactt tgactttggt ttaagtctgg gcgctgctta
      721 ctctaactct gaccgtaccg acaatcaggt tcacaaaggt actcataact ctcgttatgg
      781 agatcgcttc gatgcaactg ctggcggtga aacagcagaa gcatggaccg ttggtgctaa
      841 atacgacgct aataacgttt acttagcagc tatgtatgct gaaacccgta acatgactgg
      901 ttacggtgat gctgatgcca tcgctaacaa aacccaaaac tttgaagttg ttgctcagta
      961 tcagttcgac ttcggtctgc gcccgtccat cgcttacctg caatctaaag gtaaggattt
     1021 aggcggtgta aatagtgata acttcgacag ccagggtaat catcattaca ccaacaaaga
     1081 tctggttaaa tatgttgata ttggtatgac ctactacttc aacaaaaaca tgtccactta
     1141 tgttgattac aaaatcaacc tgctggacaa cgacgacgac ttctacaaag aaaacggcat
     1201 cgctaccgat gatatcgtgg ctgtgggctt agtctaccag ttctaatcag ccaacgcgcc
     1261 gcggcgtgtt actgtaaaac tgaacactag cctgagttcg tccctcatcg aactcaggct
     1321 tttttattga catttcatct ccctctgcgg cacacgcaat tgccgaagca tatcgataca
     1381 gcaccttacc ctgcccctcc tggcacaata acatcaacca gctactcttc aagctaacac
     1441 ccaaatgcat ttctttatga attaatccgt aacaaacacg cttcccatat agaaatattt
     1501 gcaaaaatag tgcatgaaat aagctttttc tccaccaatc cgggatataa atattcatca
     1561 gcaccacaat attccacagc actaaatctc tccccgccac cccgtacctc tgataatggt
     1621 ctaaaatcat tgaggccact tgcgacgacg ttgctcaata aggaaaattt ggcgtgaata
     1681 atattcaaca ccagtgcagc atcatcatta catacttaca cccgccgact accgctttcg
     1741 atatattgac tcaattttcc agcctggtta aaaataacga ccagctcagt cgcaaatata
     1801 gtgactaccc taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa
     1861 tccgcaggtc gatattgctg aagataatgc attcttccct tcagaatatt cgcttagcca
     1921 atataccagt cctgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa
     1981 gcataaaatt ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact
     2041 gttctcgacc ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc
     2101 aggtttcgaa ttcgaagtgg cgaccatttc cggcctgatg accaagtttg aatactgggc
     2161 tatgccgcac aaagatgaaa aagtaatgcc attctttgag cagcataaat cgttgttccg
     2221 caatccgaag aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc
     2281 aatctttgtt cctggtggtc atggcgcact tattggttta cctgaaagcc aggacgtggc
     2341 tgccgcttta cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggccc
     2401 ggcggctttt ctggcgcttc gccacggcga taacccactg aatggttatt ccatttgcgc
     2461 attcccagac gccgcagaca aacaaacgcc agagattggc tatatgccgg gtcatctcac
     2521 ctggtacttc ggcgaagaac tgaagaaaat gggcatgaat atcattaatg acgacatcac
     2581 cgggcgagta cataaggacc gtaaagttct caccggcgac agtcctttcg cagcgaatgc
     2641 gttgggtaaa ctggcggcgc aggaaatgct ggcagcttac gcgggttaat ccctgttacg
     2701 ttttaatgta agcgcataat caatcgcagc cgcgttagta ttcacgacgt ggctgcgttt
     2761 tttcacggtt aatttatggc gtactgaagc cctatgttaa tttctttgcg gtaacgtaat
     2821 ccggaacaca ttatgcttat tgagatagtg atacgtagca cttcccccat gcaattcggc
     2881 aatcgctttg actaaagaaa gaccaagtcc ctgacctacg gaatgacgcg aattatctcc
     2941 ccgccaaaat ctacggaaga gtttttcagg ctcattaatt ttcgttccag ggctggcgat
     3001 atcaatatta agatagctgt tggtatcaag aaaactggtt atatgaatac gcgatttttc
     3061 tggcgaatat cgaatagcgt taacaataag attcgataac attcgttgca gtaaaatttt
     3121 atccgcaaag atttgctgat tgcactcgac attaaagcaa atctctttct cgtctgaaag
     3181 atattcaaga tagtccaata aattttcgac ttccttattg agagaaagcg agtccagctt
     3241 caccaaaaca ttgtttttat ctgctctggc aagaaacagt atgttctctg ttaaccgcga
     3301 aatattttcc agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct
     3361 ggtttgactg agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc
     3421 atgagcgaga tcgtcagcaa actgacttag acgctcaaaa tctttgacta aagcatgatg
     3481 cattttattc aacgcctgcc caagaggttt tagttctctc ggtaatgcgc taacctcaac
     3541 aggctctcgg ctatcgttat aattcagcgc ttccgttaca ccactcaact ttttgatctc
     3601 tcgtaacccc gttcggatta acagcggact taatactgag cacagtataa tggcgacaat
     3661 gcaaattata atgctattga ttttatactg ttcaagcatg ttatgtctgg ctgaagccaa
     3721 tttagccaca gtaaccgtta atgatggcga aacttcatca atattaattc gtaaagcatc
     3781 tatctctgta tcattaataa tgcttctgta aatgccagct gcgctgattg tctcactagc
     3841 aggtatgtta tttaacatgc catcactgac atttgtccgg ttaacaattt tattgatgct
     3901 atcaccatga atgatcaaga tatcctgact aacatccatc atccggttaa agtacacagg
     3961 taacgtatct ggatttaccc catcaattaa caactgcttg atctgtgctg tccggttaat
     4021 gagtgttgta tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccagac
     4081 aattccagca ccagcaacag acagtaacaa tataaaaagc aaggttaaac ggacggttat
     4141 agacaatctt ttcattattt ttttaccgct acgaacgaat atcccatccc acggattgtg
     4201 gtaattagct tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca
     4261 tccaccgtat tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt
     4321 ctgggtataa tttcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc
     4381 gtcagtgtaa tactgatatt gtccctgctc acactttgac taacagagtc cattcttaag
     4441 ccgctgattt ctaatgttga attcaaagcg tgatgttgcc ttaattgtgc ccgaaccctt
     4501 gccagcaact cagaaaatga aaaaggtttt accagataat catttgcccc actgtccagc
     4561 cctctgactc tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt
     4621 gctgttctta acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga
     4681 atgatcaatg cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg
     4741 gcatcgatga cataacccgc ttcggaaagc ccctgcgtta cccactcctg ggtcctttga
     4801 ttatcttcaa taagtaaaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa
     4861 acagcctggt tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgctttttcg
     4921 ctatcctcaa aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac
     4981 tctccgtagt aacggctgca ttttcattac cttctttggt ctatgctgca caacaaaaca
     5041 ttcttagcgt gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca
     5101 ctcttgaaaa gaaagcggac aacggctggt tacaacttaa taccgccaaa acagataagg
     5161 atggacgaat taaggcactg tggcccgagc aaactgcaac tacgggcgat taccgtgtcg
     5221 tatttaaaac cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagatcc
     5281 ccgttgaatt tcatattaat aaagtgaacg agcattatca tgtgccttta ttacttagcc
     5341 aatatgggta ttcaacctat cgtggcagtt aatgcataaa tatgtaaaag cagatctctg
     5401 ctactgttag ggaggacact ccctgacaga ttaaaagtaa acggctcttg ctggctaaag
     5461 acaaaaaagt gtgatggctt atgaaaaaga atcaattttt aaaagaatca gatgttacgg
     5521 ccgagtcggt attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag
     5581 ctgcactttc tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg
     5641 atcgcccacc cgcccccgcc ggaaaagcgc tggagttcag caagcctgcc gcctggcaaa
     5701 ataacctgcc actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg
     5761 ggctggataa agccgatccc gccgctaatg ctggtagcct gaaaaccgat ccatggacac
     5821 tgaaaatcag cggcgaagtg gcaaaaccat tgaccctcga tcacgatgat ttaacccgtc
     5881 gcttcccgct ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg
     5941 tgccgtggat tggttttccg ctgcacaaat tgctggcgct tgccgaaccc accagcaatg
     6001 cgaagtatgt cgctttcgaa acaatttatg caccggaaca gatgccaggc cagcaggacc
     6061 gctttatcgg cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa
     6121 tgcatccact cacactgatg accgtaggtg tttatggcaa ggcgttaccg ccacaaaatg
     6181 gcgcgccggt gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg
     6241 tcagtattaa gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg
     6301 aatacggttt ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta
     6361 ccgaacgatt tattggttca ggcggcatcc tcgatgtaca gcgccagcca acgctactgt
     6421 ttaatggtta cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt
     6481 tctgaatgcg tctgacagca aaacaggtga catggctgaa agtttgcctg catcttgccg
     6541 gattgttgcc gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc
     6601 cggtgaaaga tattcagcat tttaccggtc gcactgcact gaaatttttg ctggcgacct
     6661 tgttaatcac acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc
     6721 tgttaggatt atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg
     6781 agctgggcgt gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa
     6841 cgttaggtat tatcagctgg gtaattttgc ttgctttagc gttcacttca acccaggcga
     6901 tgcagcaaaa actcggcaag cactggcaac agttgcataa tttcgtctat cttgtcgcga
     6961 tcctggcccc aatacattat ctgtggtcgg tgaagattat ttcaccgcag ccgctcatct
     7021 acgctgggct ggctgtactg cttttagcct tacggtataa gaagttgcgt tcccttttta
     7081 accggttacg caagcaagtt cacaacaaat tatctgtata acttcatatt gccgacaaag
     7141 tacgcgttat ccctgccgga tgtggcgcga gcgccctatc cggcctacgc agatcgcatc
     7201 aggttatgtt tcgtttgtca tcaacctctc atcaccagaa taatttattt tatttcaata
     7261 agatagctga atacacttga gaataccatg ctctcgtttc ctaagagttg ttgcattttg
     7321 tcatatgtta caatataaca ttacacatca tatacaatga ctctggagga aactgttttg
     7381 gcgattcgtc ttcacaaact ggctgttgct ttaggtgtct ttattgttag cgctccagcc
     7441 ttttcgcatg gtcatcactc acacggcaaa cccttaacag aggtcgaaca aaaagctgct
     7501 aatggtgttt ttgatgatgc caatgtacaa aaccgaacgc tcagtgactg ggatggagtc
     7561 tggcaatccg tttatccttt actgcaaagt ggcaaacttg accccgtctt tcagaagaaa
     7621 gcggatgcag ataaaactaa aacatttgct gaaattaaag attattatca caaaggttat
     7681 gcaacagata tcgagatgat tggcattgag gacggcattg ttgaattcca tagaaataat
     7741 gaaacaacat cctgtaaata tgattacgat ggatacaaaa tactcaccta taaatcaggc
     7801 aagaaaggcg ttcgttactt attcgaatgt aaagatcctg aaagcaaagc ccctaaatat
     7861 atacaattta gcgatcatat aattgcacca cgaaaatcat ctcattttca catttttatg
     7921 ggtaatgatt cacagcaatc tttattaaat gaaatggaaa actggccaac gtattatcca
     7981 tatcagttga gtagcgaaga agtggtcgag gaaatgatgt ctcattgagt attctcatga
     8041 taacgcctcg atgccgcttt agtaagtcat cataactgcc actggtccat ccacaaacgc
     8101 cactgaacgc aagctagcta cagacacgct catcactatg acgtgtctgt atattaataa
     8161 gctaacccgc attgagttaa ccaataacgg attccataca caatacggcc aacagagaaa
     8221 gatacctgtg ctcacgccat tgcttatatt ggctggttac aatgtgcact atcaattttt
     8281 taaataacaa attcttaaaa ccacatctgt ataaggaatt tttaaggttc gtgggatagc
     8341 ttgactgtga aaattacagg agctacaaaa atgaaccgat tctcaaaaac tcaaatttat
     8401 ttacattgga taacgctgct tttcgttgca ataacctatg ccgcgatgga actccgtggc
     8461 tggtttccta aaggtagtag tacttatctg ctgatgcgag aaacacatta caatgcgggt
     8521 atattcgttt gggtgttaat gttttcacgc ctgattataa aacaccgtta tagtgatcct
     8581 tctattgtgc caccgccacc tgcctggcaa atgaaagcgg cttcgctaat gcacatcatg
     8641 ctttatataa ccttccttgc acttcctctg ctggggattg ctttgatggc ttacagtgga
     8701 aaatcgtgga gtttccttgg tttcaatgtg tctccctttg ttaccccaaa cagcgaaatt
     8761 aaagcactga taaaaaatat tcacgaaacc tgggcaaata taggctactt tttaatcgca
     8821 gctcacgctg gcgcagcact ctttcatcac tacattcaga aagataatac tctgttacga
     8881 atgatgcctc gccgcaaata agtcgatccc ctgacaagga aatgtgcact atggacatca
     8941 acgcgtcata aatgtttacg caagtgcaca atgcaccctt tccactatca acaaggagga
     9001 gcagaaactg tgtaaatcgt acaatggtaa gacttatcaa aaatacaggt taattttggt
     9061 atttaaacct tttttacttg ttagtatgat acaaaggctt tcaaaaaagc tgggaaattc
     9121 aacaactctt ggaaattatg gactttgtca cgcggcttta atctgacggc cgcgttcctt
     9181 ttttgactca attattgacc ccttctctac gcaacttcag ttcccaccac caactttgcg
     9241 gcagtttcat aggatgaatg tctaaaagaa taatggtgag cgataagaaa acgactgaat
     9301 aactgcacat tttcgctcga aagcttcctg tcagatccat agcgaatcaa gtgctgaatg
     9361 tcacagtatc gaacagaaaa cagtgacgat ctaacccttc aagaatattc tacgattgtt
     9421 ctgtttagga aaagcaaggc gggaagtcgg gagataagtc attgataaag tggcggagag
     9481 agggggattt gaacccccgg tagagttgcc cctactccgg ttttcgagac cggtccgttc
     9541 agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat ttggcatttt
     9601 aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta
     9661 agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg caggaagcac
     9721 tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta gtcactcttg
     9781 ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa ctggattcat
     9841 taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta ggactggaat
     9901 ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc gatgatgaat
     9961 gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt cagagctggc
    10021 agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat gcttctggtt
    10081 ttaacctgat tattcatgaa gtcgctcata agctggacac ccgtaacggc gatcgtgcca
    10141 gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg
    10201 caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg agcattgatg
    10261 cttatgctgc cagtgatcct gctgaatgtt ttgccgtact ttctgaatat ttctttagcg
    10321 ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc caattttatc
    10381 aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt tcggcgacga
    10441 atgttcatta attaacaact ttgcagatta attaaccaat tgaaatgact tatgaaattt
    10501 agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga ttcctctgta
    10561 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
    10621 ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta gttgaacatt
    10681 gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt atggttgagg
    10741 gcgttttgct atggcgccat caccattttc ccctcgatta taaaacttga gttattcagt
    10801 agtctcccct cttgcaactc acacccaaaa ctgcctaacg aaaagttatt aattttcaat
    10861 catattgcta tcaggattta cattttttcg ctgtgctaga aagggcgcat ttatgttagc
    10921 tcgttcaggg aaggtaagca tggctacgaa gaagagaagt ggagaagaaa taaatgaccg
    10981 acaaatctta tgcgggatgg gaattcaact acgccgctta actgcgggta tctgcctgat
    11041 aactcaactt gcgttcccta tggctgcggc agcacaaggt gtggtaaacg ccgcaaccca
    11101 acaaccagtt cctgcacaaa ttgccattgc aaatgccaat acggtgccct acacccttgg
    11161 agcgctggaa tcggcccaaa gcgttgccga acgtttcggt atttcggtgg ctgagttacg
    11221 caaactcaac caatttcgta cgtttgctcg aggttttgat aatgtccgcc agggtgatga
    11281 actggatgtc ccggcacaag ttagtgaaaa aaaattaacc ccgccgccgg gtaatagcag
    11341 tgacaacctc gagcaacaga tagccagtac ttcacagcaa atcgggtctc tgctcgccga
    11401 agatatgaac agcgagcaag cggaaaatat ggcgcgtgga tgggcctctt ctcaggcttc
    11461 aggcgcaatg acagactggt taagccgctt cggtaccgca agaatcacgc tgggcgtgga
    11521 tgaagatttt agcctgaaga actcccagtt cgattttctc catccgtggt atgaaacgcc
    11581 tgataatctc tttttcagtc agcatactct ccatcgtact gacgagcgta cgcagattaa
    11641 caacggcttg ggttggcgtc atttcactcc cacatggatg tcgggcatca acttcttttt
    11701 cgaccacgat cttagccgtt accactcccg cgccggcatt ggcgcggagt actggcgcga
    11761 ctatctaaaa ttaagcagta acggctattt gcgactgaca aactggcgca gcgcacctga
    11821 actggacaac gattatgaag cacgcccggc caatggctgg gatgtacgcg cagaaggctg
    11881 gttacccgcc tggccgcacc ttggcggtaa actggtctat gaacagtatt atggcgatga
    11941 agtggccctg ttcgataaag acgatcggca aagtaatcct catgccataa ccgctggact
    12001 taactatacc cccttcccgc tgatgacctt tagcgcggag caacgtcagg gtaaacaggg
    12061 cgaaaatgac acccgttttg ccgtcgattt tacctggcaa cctggcagcg caatgcagaa
    12121 acagcttgac ccgaatgaag tcgctgcacg gcgtagcctt gcaggcagcc gttatgatct
    12181 ggtggatcgc aacaacaaca tcgttctgga atatcgcaaa aaagaactgg tccgcctgac
    12241 cctgacagac cccgtgacag ggaagtcagg agaagtgaaa tcactggttt cgtcgctaca
    12301 aaccaaatat gccctgaaag gctataacgt cgaagccacc gctctggaag ctgccggtgg
    12361 taaagtggtt acaacgggta aagatattct ggttaccctg ccggcgtacc ggttcaccag
    12421 tacgccagaa accgataaca cctggccgat tgaagtcacc gctgaagatg tcaaaggcaa
    12481 tttttcgaat cgtgaacaga gcatggtagt cgttcaggca cctacgctaa gccagaaaga
    12541 ttcctcggta tcgttaagta cccagacgtt gagcgcggat tcccattcaa ccgccacact
    12601 gacttttatt gcgcatgatg cagcaggtaa tcctgttatc gggctggtgc tctcgacgcg
    12661 tcacgaaggt gttcaggaca tcaccctttc tgactggaaa gataatggtg acggaagcta
    12721 tacccagatc ctgaccacag gagcgatgtc tggcacgctg acgctgatgc cacagctgaa
    12781 cggtgtggat gcggctaaag cccccgccgt ggtgaatatc atttctgttt cgtcatcccg
    12841 gactcactcg tcaattaaga ttgataagga ccgttatctc tccgggaatc ctatcgaggt
    12901 gacggtagaa ctgagagatg aaaatgacaa acctgttaag gagcaaaaac agcaactgaa
    12961 taccgcagtc agcatcgaca acgtgaaacc tggtgtcact acagactgga aagaaaccgc
    13021 agatggcgtc tataaggcaa cctataccgc ctataccaaa ggcagtgggc ttactgcgaa
    13081 gctgttaatg caaaactgga atgaagattt gcataccgct ggatttatca tcgacgccaa
    13141 cccgcagtca gcgaaaattg cgacattatc tgccagcaat aatggtgtgc tcgccgatga
    13201 gaatgcagca aacaccgtct cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga
    13261 tcataccgtc acgtttgcgg tattaagcgg atcggcaact tcctttaaca atcaaaacac
    13321 cgcaaaaacg gatgttaatg gtctggcgac ttttgatctg aaaagtagta agcaggaaga
    13381 caacacggtt gaagtcaccc ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt
    13441 cggcgactcg agtaccgcgc aggttgatct gcagaagtcg aaaaatgaag tggtcgctga
    13501 cggcaatgac agtgccacaa tgaccgcgac agtccgggat gcaaaaggca acctgctcaa
    13561 tgacgtcaag gtcaccttca atgttaattc agctgcagcg aaactgagcc aaaccgaagt
    13621 gaatagccac gacgggatcg ccacagctac gctgaccagt ttgaaaaatg gtgattatac
    13681 ggttacggcc tctgtgagct ctggttctca ggctaatcaa caggtgattt ttatcggtga
    13741 tcaaagtact gcagccctga ccctcagtgt gcccccaggc gagatcaccg tcaccgacac
    13801 agcgcctcaa cagctgactg caaccttgca ggataaaaat ggcaacccac taaaagataa
    13861 agaaatcacc ttctctgtgc caaacgacgt cgcaagtcgg ttctcgatta gcaacggcgg
    13921 aaaaggcatg acggatagca acgggactgc aatcgcctcc ctgaccggca cgttagcggg
    13981 cacgcatatg atcacggctc gtctggctaa cagcaatgtc agcgatacac agccaatgac
    14041 gtttgtggcg gataaagaca gagcggttgt cgttctgcaa acatcgaaag cggaaatcat
    14101 tggaaatggc gtggatgaga cgactctgac ggcaacagtg aaagatcctt ttgataacgt
    14161 ggttaaaaat ctgtcagtag tcttccgcac ctcccccgca gacacgcaac tgagtctgaa
    14221 cgcgcgtaat actaatgaga acggtattgc tgaagttacc cttaagggca cggttttggg
    14281 ggtttacaca gccgaagcca cactgcctaa cggcaacaga gatacgacga cagtcaatat
    14341 tgcgcccgat gccagcaacg cgctggtcac gctgaacatc ccggctcaac aggtggtgac
    14401 gaataacagc gacagcgtgc agctgacggc gacggtgaaa gatccgtcga atcatccggt
    14461 ggcgggaata acggtgaact tcaccatgcc acaggacgtg gcggcaaact ttacccttga
    14521 aaataacggt attgccatca ctcaggccaa tggcgaagcg cacgtcaccc tcaaaggcaa
    14581 aaaagcgggc acgcatacgg ttaccgcaac gctgggtaac aataatgcca gcgatgcgca
    14641 accagtcacc ttcgtggcgg ataaggacag cgcagttgtc gttttgcaaa catcgaaagc
    14701 ggaaatcatt gggaatggcg tggatgagac gactctgacg gcaacagtga aagatccttt
    14761 tgataacgca gtaaaagatc tacaggtcac cttcagtacc aacccggcag atactcaact
    14821 tagtcagagc aaaagcaata ctaacgacag tggtgtggct gaagttacgc ttaagggcac
    14881 ggttttgggt gttcatacag tcgaagccac actgcttaac ggcaatggct atacgacgac
    14941 agtcaatatt gcgcccgatg ccagcaacgc gcaggtcacc ctgaacatcc ctgctcaaca
    15001 ggtggtgacg aataacagtg acagcgtgca gcttacggcg atggtgaaag atccgtcgaa
    15061 tcatccggtg gcggggataa cggtaaactt caccatgcca caggacgttg cggcaaactt
    15121 tacccttgaa aataacggta ttgccatcac tcaggccaat ggggaagcgc atgtcacgct
    15181 gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg ctgggtaata acaataccag
    15241 tgattcgcag ccggtaacat ttgtggcgga caaaacctcg gctcaggttg tcctgcagat
    15301 gtcaaaagat gagatcacag gtaatggcgt cgataacgca acgctaactg caacggttaa
    15361 agatcagttc gacaatgagg tgaataatct tccgataaca ttcagctcag cctcttcagg
    15421 actcaccctg accccgggag taagtaatac caatgagtct ggcatcgcgc aggccactct
    15481 cgcaggcgtt gcctttggtg agcagacggt cactgcatca ctggctaata atggtgccag
    15541 cgacaacaaa actgtgcatt ttattggcga cacagcggcg gcaaaaatta tcgagttgac
    15601 gcctgtccca gacagcataa tcgccggtac cccgcagaac agctccggca gcgtcatcac
    15661 cgccacagtc gttgataata atggctttcc ggtgaaaggt gtgactgtga acttcaccag
    15721 caacgcagcg acagccgaaa tgacgaacgg cggtcaagcc gtgacgaacg aacagggtaa
    15781 ggctaccgtc acttatacca atacccgctc ctcgatagaa tcaggagcga gaccggatac
    15841 cgttgaggcc agtctggaaa atggtagctc cacgcttagc acatcaatta atgtcaacgc
    15901 tgatgcgtct acggcacatc tcaccttgct acaggcactt tttgatacag tctccgcagg
    15961 cgacactacc aatctgtata ttgaggtgaa ggataattac ggcaacggag taccccagca
    16021 ggaggtaacc ctcagcgttt cgccgagtga aggcgtgacc ctcagtaata acggtataaa
    16081 tactaccaac cacgacggct atttatacgc aagctttacc gctacaaaag ccggggttta
    16141 tcaagtgacg gcaaccctcg acaatggcga ttcgatgcaa caaacagtga cctatgtgcc
    16201 gaacgtcgcg aatgccgaaa tcacgctggc agcctcgaag gatccggtga ttgccgacaa
    16261 taacgatctc acgacactaa cagcaacagt cgctgataca gagggcaatg cgatagccaa
    16321 cactgaagta acatttactc tgccggaaga tgtgaaggcg aacttcacgc tgagcgatgg
    16381 cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc acgctgaaag gtacaaaagc
    16441 aggcgctcat actgttacag catcgataac tggcggtaag agtgagcagt tggtggtgaa
    16501 ctttactgcg gatacgctca ctgcgcaggt taatcttaac gttaccgagg acaattttat
    16561 cgctaataac gtcgggatga ccaggctgca ggcaacagtg actgatggaa acggcaaccc
    16621 gttagccaat gaggcggtga cattcacgct accggcagat gtgagcgcaa gctttactct
    16681 cggacaaggc ggttccgcca ttactgatat caacggcaag gctgaagtta cactgagcgg
    16741 tacaaaatcc ggcacctatc ccgtgacagt tagcgtgaac aattatggtg tcagtgatac
    16801 gaaacaggtg actttgattg ccgatgcagg taccgcaaaa ctggcttcct taacctctgt
    16861 atattcattc gtcgtcagca cgaccgaggg cgcaaccatg acggcaagcg tcactgacgc
    16921 taacggcaac ccggtagaag gtataaaagt taatttccgc ggaacttccg tcacgctaag
    16981 cagcaccagc gttgaaacgg atgatcgggg tttcgctgaa attcttgtga caagcaccga
    17041 ggtcggactg aaaacagttt cagcctctct ggcagataaa cctactgaag tcatctcgcg
    17101 attactgaat gcaaaagcag atattaattc tgcaacgatt accagtctgg agatacctga
    17161 aggtcaggtc atggtcgcac aagacgtagc agttaaagct cacgtcaacg accagtttgg
    17221 caatccgatt cttaatgaat ctgtaacatt cagtgcagcg ccaccagagc acatgaccat
    17281 cagccaaaat attgtctcta ctgatacgca tggtatagcc gaggtcacta tgacgcccga
    17341 aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat ggatcctctt atgagaagga
    17401 tctggtggta atcgatcaaa aactgacact ctcggcgtcc agcccgctta tcggtgtcaa
    17461 ttccccaaca ggtgcaactc tgacggcaac gctaacttct gcaaatggca ctccagtgga
    17521 gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg acgttaagtg gcggaaaagt
    17581 gagaaccaac tcttcaggtc aggctccagt cgttctgacc agcaataaag tcggtacata
    17641 tacggtgact gcatcgttcc ataacggtgt aacaatacag acacagacaa tcgtgaaagt
    17701 cactggcaac tcaagtaccg cccatgttgc tagctttatc gctgatccat cgactatcgc
    17761 cgccaccaac actgatttaa gtaccttaaa ggcaacggtt gaggatggca gtggtaacct
    17821 gatcgaaggt ttaactgtgt acttcgcctt aaaaagcggc tctgccacat taacgtcatt
    17881 aacagcggtg accgatcaaa acggaatcgc gacaacaagc gtgaaaggag cgatgacagg
    17941 tagcgtcacg gtaagcgcag tcacgaccgc tggtggaatg caaacagtag atataacgct
    18001 ggtggcaggc ccggcagacg cctcaaagtc agtccttaag aacaatcggt catcattgaa
    18061 aggagacttt accgatagtg ctgagctaca tcttgttctg cacgatatat caggcaatcc
    18121 gatcaaagtt tctgaagggc tggaatttgt gcagtcaggt accaacgtgc cctatgtgca
    18181 agttagtgca attgactaca gtaaaaattt ctcaggcgag tacaaagcca ctgttacagg
    18241 cggcggagag ggtatcgcaa cgctgatccc tgtattgaat ggtgttcatc aagctggtct
    18301 gagtaccaca atacaattca ctcgcgcaga agacaaaata atgagcggta cagtgtcagt
    18361 caatggtact gacctaccga caactacatt cccttcgcag ggattcaccg gggcgtatta
    18421 tcagttgaat aatgacaact ttgcccctgg aaaaacggcg gctgattatg agttttcaag
    18481 ctctgcctcc tgggtcgatg ttgatgctac cggtaaagtg acatttaaaa atgtcggcag
    18541 caattgggaa aggattacgg cgacgccaaa atcaggaggc cctaactatg tatacgaaat
    18601 ccgtgtgaag agttggtggg tgaacgccgg cgaggctttc atgatataca gccttgctga
    18661 aaatttttgc agcagcaatg gctacacgct ccccagagca aactatttaa accatagtcg
    18721 ttcccgaggc atcgggtcac tgtacagtga atggggagat atggggcatt acacgactga
    18781 ggctggtttt caatcaaata tgtattggtc atctagtccc gcaaactcaa acgaacaata
    18841 cgtagtttcc ctggcaacag gtgatcaaag cgtatttgaa aagcttgggt ttgcttatgc
    18901 gacatgttat aaaaacctct gattttccgt tataatttca taaagagtgc tccattcggg
    18961 gcactctttt ttacattccc tctatagagc ttgctcttct ctcaccgaaa atgaataaat
    19021 aaaaatgcga taccgccaga ttcgcgttcg gtgtgactat gatcgtatcc ggtgagtaca
    19081 gctttgatcc aaaggctggt tttctgtgat gattgaccag cctcagagta ttgatgacac
    19141 caccgttcag aacagatcgg cattactatg cataagtact gtctcatatt ctcgccaaaa
    19201 cactttttaa tgcctttgcg aattcatcca gacttatatt gtcaattttt tgcccaacga
    19261 cgactgactc cgaaacatcg gccattgcgg gccatcgcgt tttgtaatca gcataaattg
    19321 caacaacggg ggtattatag gcacttgcca tatgtaatat tgaagtgtca ggagttattg
    19381 cgatataagc atcttttatg attgctgctg aacgtttaat cgatggtgac aaggataaac
    19441 ggtagacatt attataacta ttcactagtt ccctggcttt atcttcccct tttggtccat
    19501 gaacgatcac aattggcata tctgtttcac tttgaatttt ttctattaga ttttctgcaa
    19561 tcgatagtga aaatgtacga tcctgcgagc taccttctaa attaagcgca atgtaagatc
    19621 ctaacgatcg catttcctcg cgcacctcat cgagtacatc ctcacttagt ggcaattcat
    19681 atattggcct gactgctgga aaacccgcct cacgcatcat aaatgcccat gtcatgggta
    19741 cgggagcccg gagtttctga tccatccggg acgcgttctt acacaaaggg gaatagctct
    19801 tcatggttaa accaacaacc tgaaaattcg tttttgcttt caactgactg ataaataaca
    19861 tcgttttcag gttcttttta cgcatcgcct ctatacaaag atccggcgta ccgtattgct
    19921 gtgttatgtt cttcgctaaa tcttttattt cttttaatgt tgcgtgatcc tgcatagtca
    19981 ttgtgactaa tgttaattta gtctgttcaa gtttgagcgc attaaagact tctaaattaa
    20041 ttatcgacgt aacaattagt agatgcttaa ttttatgcaa ttcaagcgcc cgaataactg
    20101 gaaagatggc catagcatcg ccaatctgat cgggaatatg gatgacaaca aaatctgttt
    20161 tttcaatatt gaaattataa gctttataat cgtagtaact aaatgcaata cgtctcaaca
    20221 atgatgctaa aaacatacct aacctcgcct ccctactggt tataatgcaa agcagtctat
    20281 cagactcatc agggtgccat tttgtgcata tgcggacttt tatgtttcat atctcgaacc
    20341 tgtgggtcat ctgcttaatc cttaaacaac accagcaact cccgcgcttt catcttccag
    20401 cgaatttttc atgttgccgc taatcagcca taaaatcatt tgcagatgcg ctctgtcgag
    20461 gtagtctcat aaggttcgtt tatagatcga cggcaatgtg agttaccttt tccatactaa
    20521 ttataaaaag acagtacaaa caggatcatt atggactcca cgctcatctc cacccgtccc
    20581 gatgaaggga cgctttcgtt aagtcgcgcc cgacgagctg cgttaggcag tttcgctggt
    20641 gccgtcgtcg actggtatga ttttttactc tatggcatta ccgccgcact ggtgtttaat
    20701 cgcgagtttt tcccgcaagt aagcccggct atgggaacgc tcgccgcatt tgccaccttt
    20761 ggcgtcggat tccttttccg tccgctcggc ggtgtcattt tcggtcactt tggcgaccga
    20821 ctgggacgta agcgcatgtt aatgctgacc gtctggatga tgggcatcgc gacagccttg
    20881 attggtattc ttccttcatt ctcgaccatt gggtggtggg cacctatttt actggtgacg
    20941 ctgcgtgcca ttcagggatt tgccgtcggc ggcgaatggg gaggcgcggc gttgctttcc
    21001 gttgaaagtg caccgaaaaa taaaaaagcc ttttacagta gcggtgtaca agttggctac
    21061 ggtgtaggtt tactgctttc aaccggactg gtttcattga tcagtatgat gacgactgac
    21121 gaacagtttt taagctgggg ctggcgcatt cctttcctgt ttagcatcgt actggtactg
    21181 ggagcattgt gggtgcgcaa tggcatggaa gagtccgcgg aatttgaaca acagcaacat
    21241 aatcaagctg ccgcgaaaaa acgcatcccg gttatcgaag cgctgttacg acatcccggt
    21301 gctttcctga agattattgc gctacggctg tgcgagttgc tgacgatgta catcgttacc
    21361 gcctttgcac ttaattattc aacccagaat atggggttac cgcgcgaact tttccttaat
    21421 attggtttgc tggtaggtgg attaagctgc ctgacaattc cctgttttgc ctggcttgcc
    21481 gatcgttttg gtcgccgcag ggtttatatc acaggtgcgt taatcggaac gttgagcgca
    21541 tttcctttct ttatggcgct tgaagcacaa tctattttct ggatagtttt cttctccata
    21601 atgctggcaa acattgcgca tgacatggtg gtgtgtgtgc aacaaccgat gtttaccgaa
    21661 atgtttggtg ccagttatcg ctatagtggt gctggagtcg gttatcaggt tgccagtgtg
    21721 gttggcggtg gatttacacc ttttattgcc gctgcgctca tcacttactt tgccgggaac
    21781 tggcatagcg tcgccattta tttgctggct ggatgtctga tttccgcaat gaccgctttg
    21841 ttgatgaaag acaatcaacg cgcttgatag tctggcgaag atcatccgat cttcgcctta
    21901 cacttttgtt tcacattcct gtgacatact atcggatgtg cggtaattgt atggaacagg
    21961 agacacacat gaataataag ggctccggtc tgaccccagc tcaggcactg gataaactcg
    22021 acgcgctgta tgagcaatct gtagtcgcat tacgcaacgc cattggcaac tatattacaa
    22081 gtggcgaatt acctgatgaa aacgcccgca aacaaggtct ttttgtctat ccatcactga
    22141 ccgtaacctg ggacggtagc acaaccaatc cccccaaaac gcgcgcattt ggtcgtttta
    22201 ctcacgcagg cagctacacc accacgatta ctcgccctac tctctttcgt tcgtatctca
    22261 atgaacaact tacgttgctg tatcaggatt atggtgcgca tatctcggtg caaccctcgc
    22321 agcatgaaat cccttatcct tatgtcatcg atggctctga attgacactt gatcgctcaa
    22381 tgagcgctgg gttaactcgc tatttcccga caacagaact ggcgcaaatt ggcgatgaaa
    22441 ctgcagacgg catttatcat ccaactgaat tctccccgct atcgcatttt gatgcgcgcc
    22501 gcgtcgattt ttccctcgcg cggttgcgcc attataccgg tacgccagtt gaacattttc
    22561 agccgttcgt cttgtttacc aactacacac gttatgtgga tgaattcgtt cgttggggat
    22621 gcagccagat cctcgatcct gatagtccct acattgccct ttcttgtgct ggcgggaact
    22681 ggatcaccgc cgaaaccgaa gcgccagaag aagccatttc cgaccttgca tggaaaaaac
    22741 accagatgcc agcatggcat ttaattaccg ccgatggtca gggtattact ctggtgaata
    22801 ttggcgtggg accgtcaaat gctaaaacca tctgcgatca tctggcagtg ctacgcccgg
    22861 atgtctggtt gatgataggt cactgtggcg gattacgtga aagtcaggcc attggcgatt
    22921 atgtacttgc acacgcttat ttacgcgatg accacgttct tgatgcggtt ctgccgcccg
    22981 atattcctat tccgagcatt gctgaagtgc aacgtgcgct ttatgacgcc accaagctgg
    23041 tgagcggcag gcccggtgag gaagttaaac agcggctacg tactggcact gtagtaacca
    23101 ctgatgacag gaactgggaa ttacgttact cagcttctgc acttcgtttt aacttaagcc
    23161 gggccgtagc aattgatatg gaaagtgcaa ccattgccgc gcaaggatat cgtttccgcg
    23221 tgccatacgg gacactactg tgtgtttcag ataaaccgtt gcatggcgag attaaacttc
    23281 ccggccaggc taaccgtttt tatgaaggcg ctatttccga acacctgcaa attggcattc
    23341 gggcaatcga tttgctgcgc gcagaaggcg accgactgca ttcacgtaaa ttacgaacct
    23401 ttaatgagcc gccgttccgg taataagttc tgttcctttt tctcagaaat agatgtgaag
    23461 gatttagtaa gtcatctggc aaatagcctg caataaagca ggctgtttgc ttccttaggc
    23521 ggatacgagt tttattatcg tcttaatgat tttcacatat taaaagcatg tatgctttca
    23581 aaacacaatt ataa
//
LOCUS       DAPGRN010000070        23290 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPGRN010000070 DAPGRN010000000
VERSION     DAPGRN010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.070"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1135
                     /locus_tag="RHW33_002942"
     CDS             <1..1135
                     /locus_tag="RHW33_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV1646423.1"
                     /translation="NKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSND
                     VSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSS
                     YNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLC
                     LASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDV
                     LTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDI
                     IQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQM
                     TISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1316..2806
                     /locus_tag="RHW33_002943"
     CDS             1316..2806
                     /locus_tag="RHW33_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV1646424.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYLDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIASQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2849..3352
                     /gene="yeaK"
                     /locus_tag="RHW33_002944"
     CDS             2849..3352
                     /gene="yeaK"
                     /locus_tag="RHW33_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV1646425.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGADFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     INFRRTA"
     gene            complement(3353..3457)
                     /locus_tag="RHW33_002945"
     CDS             complement(3353..3457)
                     /locus_tag="RHW33_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646426.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3627..4073
                     /locus_tag="RHW33_002946"
     CDS             3627..4073
                     /locus_tag="RHW33_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV1646427.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4030..4851)
                     /gene="nimR"
                     /locus_tag="RHW33_002947"
     CDS             complement(4030..4851)
                     /gene="nimR"
                     /locus_tag="RHW33_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV1646428.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4948..6129
                     /gene="nimT"
                     /locus_tag="RHW33_002948"
     CDS             4948..6129
                     /gene="nimT"
                     /locus_tag="RHW33_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV1646429.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQIGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6184..6531
                     /locus_tag="RHW33_002949"
     CDS             6184..6531
                     /locus_tag="RHW33_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV1646430.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNATQ
                     NHALVLADWLRSL"
     gene            complement(6553..6807)
                     /gene="yoaF"
                     /locus_tag="RHW33_002950"
     CDS             complement(6553..6807)
                     /gene="yoaF"
                     /locus_tag="RHW33_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV1646431.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6990..8015
                     /gene="dgcP"
                     /locus_tag="RHW33_002951"
     CDS             6990..8015
                     /gene="dgcP"
                     /locus_tag="RHW33_002951"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV1646432.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            8048..8146
                     /locus_tag="RHW33_002952"
     CDS             8048..8146
                     /locus_tag="RHW33_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV1646433.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8149..8223
                     /gene="yoaJ"
                     /locus_tag="RHW33_002953"
     CDS             8149..8223
                     /gene="yoaJ"
                     /locus_tag="RHW33_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV1646434.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8281..8529)
                     /locus_tag="RHW33_002954"
     CDS             complement(8281..8529)
                     /locus_tag="RHW33_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV1646435.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8677..8859)
                     /locus_tag="RHW33_002955"
     CDS             complement(8677..8859)
                     /locus_tag="RHW33_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV1646436.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8863..9222)
                     /gene="yeaR"
                     /locus_tag="RHW33_002956"
     CDS             complement(8863..9222)
                     /gene="yeaR"
                     /locus_tag="RHW33_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV1646437.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9395..10033)
                     /gene="leuE"
                     /locus_tag="RHW33_002957"
     CDS             complement(9395..10033)
                     /gene="leuE"
                     /locus_tag="RHW33_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV1646438.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10160..11083)
                     /gene="dmlR"
                     /locus_tag="RHW33_002958"
     CDS             complement(10160..11083)
                     /gene="dmlR"
                     /locus_tag="RHW33_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV1646439.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11186..12271
                     /gene="dmlA"
                     /locus_tag="RHW33_002959"
     CDS             11186..12271
                     /gene="dmlA"
                     /locus_tag="RHW33_002959"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV1646440.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            12522..14132
                     /gene="yeaV"
                     /locus_tag="RHW33_002960"
     CDS             12522..14132
                     /gene="yeaV"
                     /locus_tag="RHW33_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV1646441.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            14164..15288
                     /gene="yeaW"
                     /locus_tag="RHW33_002961"
     CDS             14164..15288
                     /gene="yeaW"
                     /locus_tag="RHW33_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV1646442.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15344..16309
                     /gene="yeaX"
                     /locus_tag="RHW33_002962"
     CDS             15344..16309
                     /gene="yeaX"
                     /locus_tag="RHW33_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV1646443.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALISSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16356..17471)
                     /gene="rnd"
                     /locus_tag="RHW33_002963"
     CDS             complement(16356..17471)
                     /gene="rnd"
                     /locus_tag="RHW33_002963"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV1646444.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMIETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17553..19238)
                     /gene="fadD"
                     /locus_tag="RHW33_002964"
     CDS             complement(17553..19238)
                     /gene="fadD"
                     /locus_tag="RHW33_002964"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV1646445.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19443..20024)
                     /gene="yeaY"
                     /locus_tag="RHW33_002965"
     CDS             complement(19443..20024)
                     /gene="yeaY"
                     /locus_tag="RHW33_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV1646446.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20064..20759)
                     /gene="tsaB"
                     /locus_tag="RHW33_002966"
     CDS             complement(20064..20759)
                     /gene="tsaB"
                     /locus_tag="RHW33_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV1646447.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20817..22727)
                     /locus_tag="RHW33_002967"
     CDS             complement(20817..22727)
                     /locus_tag="RHW33_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV1646448.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGELPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22859..23203
                     /locus_tag="RHW33_002968"
     CDS             22859..23203
                     /locus_tag="RHW33_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV1646449.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5725 a         5940 c         5644 g         5981 t
ORIGIN      
        1 taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg gccttatcta
       61 tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac tgatccagac
      121 aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta tttgtttaac
      181 ctcgctggcg ctcttttgtt atggaaaaga caacatcctt gacaacaata agaaaaaaac
      241 tggcattctg ttgctggcgc tgattccttt tttagttttt ccccttctgg cacacaatct
      301 gagcagttat aacgctgact attctttgta tgtcgtcgat tactgtccgg acaaccatac
      361 tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat ttttactgtt
      421 ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag cattattatg
      481 tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata attataccat
      541 ctggtatatc agtcgcggga ttgaagtctc cagtaaactg tttgttgtgt cttttctgat
      601 ttataacatt tttcaggagc tgcaactctc cagcaaactg gcagttcatg atgtgctgac
      661 caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt cgcgacctgt
      721 tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca tcaatgccca
      781 atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta tccagcaaag
      841 catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct tgctatttac
      901 cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa atgtcgaact
      961 cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca gtattggcac
     1021 ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag cagataaagc
     1081 cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata tttaagtgca
     1141 aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta ctattacccc
     1201 taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg tgccaggccg
     1261 caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat ttatcatgaa
     1321 attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc tgacatcttc
     1381 cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc tgcgctatat
     1441 cgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga gtattgccgc
     1501 gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc
     1561 catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga ctgcccataa
     1621 atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca caattgtgga
     1681 agctgcagca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc accaggatga
     1741 ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg atctcaatga
     1801 ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgcgatga ataactggtc
     1861 cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg tatttaccgg
     1921 gcgtaccgta ttatcgagca tttacctgga tcagcgtact aaacagaacg tgatgagttt
     1981 gctgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg atattaacaa
     2041 aaacaatctg cggaatatct tttacactca tgaccgccct ctcctctggc gttttctcaa
     2101 tgtcacgcta accgatacag attcggggcg cgacattatc atcaaccaga gcgaagataa
     2161 tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg tctcgttatc
     2221 cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct ggattttgac
     2281 ggcgttaatt ttgctgaata tggtgcggat gcacttccgc ttataccaaa atgtgtcgcg
     2341 agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa cccctgaact
     2401 ggagcagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta ttgcaattga
     2461 catggacaag ttaaagctaa taaatgacac cctcggtcat caggaagggg atttagcgat
     2521 tacgttgtta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg ccatccgact
     2581 cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg catcacaact
     2641 gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaga tcggcttctc
     2701 ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata aagcttccga
     2761 tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac cttctgtggt
     2821 tgtttgcttg taatctcagg agcatgaaat gactgaaatg gctaaaggaa gcgtgacgca
     2881 tcagcggtta atcgctttat tatcacaaga aggtgcagac ttccgcgtcg ttacccatga
     2941 agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctttag gccagggggc
     3001 taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc tggcgatcct
     3061 cgctgcggat caacaggccg atctgagcca actcgccagc catataggcg gattacgcgc
     3121 ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg gcgcgatccc
     3181 ccctttcagc ttccatccaa aactcaaact ggttgccgac cccctactct tcgaacgatt
     3241 tgatgaaatc gccttcaatg caggcatgct ggataaatcc gttattttga aaaccgcgga
     3301 ttatttgcgc attgcgcaac cagaactgat caatttccgc cgcactgcgt aactagcctg
     3361 tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatggcaaaa aacgatgacg
     3421 taataatcag tgtctcgacg aacatttgat cgttcatagt atgccccagg gagatagctg
     3481 ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttccc ctgatttttt
     3541 atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag taagcaggct
     3601 tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc ctgcttgggt
     3661 tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt ctggtgttaa
     3721 tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa caggggctaa
     3781 gtatcgggat aataattctg acgattggcg ttatggcccc catcgccagc gggacgctac
     3841 caccctcaac cctgattcac tcgtttctga actggaaatc gctggtggca attgcagtag
     3901 gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc cagccgcaac
     3961 tggtcgccgg gctgctggta ggcactgttt taggcgtggc gctgtttcgc ggcgtaccgg
     4021 tcggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag tagttaatcc
     4081 cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa taaaggccgt
     4141 tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt tcactaatcc
     4201 ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca accccgtctc
     4261 ttttactatc agtcgggcaa gattgcgttc actcatcgca aaaaagccag cccattgccc
     4321 caatgacccc cactcgacgg gccctttcgc catcatctcc accatcgtgc ggattttagg
     4381 atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt catcaaacag
     4441 gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc tgtttgccag
     4501 ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg ttgtcggcat
     4561 tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg tcacttgatt
     4621 gctatgttcc accccgcccg gtatccaaac cgcatattgc ggcggcacca tccacaaagc
     4681 attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc ctttgcggtg
     4741 ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg cgaccgcggc
     4801 ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca ttttgtccga
     4861 atatagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac ggtaaggtac
     4921 agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag cggcaaaaac
     4981 aggattgtcc ttatcgctgg cattctgatg attgccacta cattacgcgt caccttcacc
     5041 ggcgcagcac cgttactcga tacgattcgt tccgcttact cgctgacgac tgcgcaaatc
     5101 ggcttattaa ccactctgcc attattggcc tttgcgctaa tctcacctct ggctgccccg
     5161 gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt gatctgtgca
     5221 ggtatcgcaa ttcgctctct cccttcgcct tacttattat ttggcggtac agcggtcatt
     5281 ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg cgatttccct
     5341 cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc ggcggccctg
     5401 ggatcggcta tggtagtgcc gctggctttg aacggttttg gctggcaagg cgcgttgctc
     5461 atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg gcgaagccaa
     5521 caacatgcaa atttgagtac ttcacgcgcc ttacatactc ggggtatctg gcgttcgccg
     5581 cttgcctggc aggtaacatt gtttcttggg atcaactcac tggtctatta cgtgattatt
     5641 ggctggcttc cggcgatcct catcagtcac ggctatagcg aagcacaggc gggttcactg
     5701 catggtttgc tgcaactagc cacagcagct cccggtttgc tgatcccgct tttcttacat
     5761 catgtgaaag accagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc agtgggcgcg
     5821 gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt cggttttggt
     5881 tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag ttctgcgcat
     5941 caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc agcctgtggg
     6001 ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc actaatgggt
     6061 gttgcaatac tttcactact gatggcgatt ttcggacttt gcgccggaag agacaaagaa
     6121 attcgctaat atccggtgct atagtgacgt gacaaatcat gcgtgaaagg gagaacaaac
     6181 acgatgaata ttcagtgcaa acgcgtttat gatccggcag aacagagcga tggttatcgc
     6241 atactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc ccttgatgag
     6301 tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg cgaagtcgtc
     6361 gattttgcaa cctttcgcga gcaatatctt gcagaactgg cacaacacga gcaagaagga
     6421 aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctactc agcaaaaaac
     6481 gccacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg attttagtac
     6541 agcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg cccattcatc
     6601 aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg tctgcactgg
     6661 aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca taccaatctg
     6721 cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga ccagcaagca
     6781 aggcagaaca aaactgataa ttttcattta ttgatctcac atatatatcc aagcttagag
     6841 tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca aaacatatta
     6901 cgccttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta aataatcagc
     6961 atttacccgc cgtatcctgg agttgttccg tgtcagatca gattatcgcc cgcgtctcgc
     7021 aatcccttgc caaagaacag tcactggaaa gtctggtccg acagcttctg gagatgctgg
     7081 aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa gcgcgcctgc
     7141 agcatattat gtttgcccgt aacagccaga aaatgcatat cccggagaat tttaccgtct
     7201 cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ctgctttttc agcgatgaag
     7261 tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc acatttctga
     7321 gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc gccagcagtg
     7381 agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc ggactgattg
     7441 cacaatatat tcaaaaagag gcgttggttg aacaactgcg cgaagccaat gccgcgctga
     7501 ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg atttttgaaa
     7561 atctgacgac gctgttttcc ctcgcccggc atcttaacca taagataatg atcgcgttta
     7621 tcgatctgga taacttcaaa ttaatcaatg atcgttttgg tcataatagt ggcgatctgt
     7681 ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa gttattggtc
     7741 gtctcggtgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg gatatttcgt
     7801 cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt gatgttgatt
     7861 tgtattatcc tggtgccagt cttggcatag tagaagtcga tcctgaaacg accgatgcag
     7921 acagtgccct acatgctgcc gatatcgcga tgtatcagga gaaaaaacac aaacagaaaa
     7981 caccttttgt cgcgcattca gcgccacatt cctgaggcgt attcacatcc ttttgattgg
     8041 tgataacatg cgaatcggta ttatttttcc ggttgtaatc ttcattacag cggtcgtatt
     8101 tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat gaaaaaaaca
     8161 acgatcatta tgatgggtgt ggcgattatt gtcgtactcg gcactgagct gggatggtgg
     8221 taacgtcacc tctaaaaata gcaaaggctg cctgtgtgca gcctttgtgc aatttacgcg
     8281 ttaactttta atcttcctgt agataaatag cacgacaatc gcaccaataa cggcaaccac
     8341 gaagctgcca aaattgaagc catcgacttt accaaagcca aacagcgtgc tgatccaacc
     8401 gccgactacg gcaccgacta tccccagcag gatagtcata aagaatccac caccatcttt
     8461 acctggcatg atccacttcg ccagaatacc ggcaataagc ccaaaaataa tccatgacag
     8521 aatgcccatt gtttcctcac ttatctgttt tgcattagcg ggttagtcgc tgataaaaag
     8581 catagcacaa catcgggagg gcaagatttg tgacgagcat cacggaggat ttttttgcga
     8641 tggcgcagaa attgcgccat caacgatcag tgataattac caaccacaaa catcatgttc
     8701 gttttccgtg tcataagaac gcacggtatt caccagatct tttatcactt cagccgccac
     8761 ttctggcacc agcaaagtca tcggcgtctc tgtttcataa tcgacagaaa cgccattgct
     8821 gttattggtg acggtcacgg tatacgttgc tttgcccatg attcatttcc cgttatgaat
     8881 gactttccgt tgttgcgcac cttccatcag gacttcagga gccacgaaga agtcaatgtt
     8941 gaaataagta tcgtcagtca tggcttcaat gttgtgccac ttttctggag ggaacaccgc
     9001 gaactgcccc gcttcgataa ggatcacctg atcaggctct gcactgtgtt catcagcgta
     9061 gccgagatat ttgaccgccc catgcataac ggaaaggcgt gggtaaaccc ccgggcgcgt
     9121 tcctttatca agatgacgtt cgaatattcc ggcaggcgca gtttgtttat tccagaaagg
     9181 cgttgagcgc gtatgaatat aattctgtgg gatttgcagc atccttttcc ctccttcggt
     9241 gaatgcgctg aaaacggttt attccagccg tttcagggta cgcctgataa tttgcatttt
     9301 aaataccatt tattggttac tttttagcac catatcagag aagaatcagg gaggattata
     9361 gatgggaagc ccgcgacaac gcgggctgaa agcatcagga ttgcagcgtc gccagtcggg
     9421 cagcgaaacc cacgaacatc aaaccaatca gtgagttgcc cactttagcc agtttctttt
     9481 tggtacgtat gtactgcgtg acaaaagcac cagaaataat caggaagctc aaatagcaga
     9541 aactcaccag ttccagcgtc gtcgccagaa taaagaatga aattcccgta tgtggggcat
     9601 taacatcgat aaactgtacg aaaaacgaca catagaacaa aatggctttc ggattagtca
     9661 ggctcaaaat taacgcgcgt ttaaaaatag caccgtattg gggctcatcg gatttggcct
     9721 cgctattttt acccttcagg gtcgcgtaaa gaattttact ccccagatag agcaaataaa
     9781 acgcaccaag ataacgtaca atgttgaata atatcggggt ggtcttaatt aatgtcgcca
     9841 ctccagccca tgccagaaac atcaataccg catcgccaat aaatacaccg caggccgcaa
     9901 gataaccgcc tttcataccg ctactgacgc tatttttgag tacaaacagg gtatttggcc
     9961 ctggcaccaa cacaataaaa atggccccaa ccagataggt ccagtaattc agaaccccgt
    10021 attcagcgaa cacattaacc tctttaatta tctttcgatc atgcgcgatt aaaggtgaat
    10081 atgctaacca atctgtagcg gcttagaaag gagaaaatca ggttttaacc tgatatcaac
    10141 ccgataattg aatcatgaat caggcatgct ccagtgaaaa attcgggtag tgctcggcaa
    10201 aatactgccg taaaaattct accgttatcc gcactttcgc tgacgtcgcc agccttgaaa
    10261 cataaacggc ccagacgttc gctggctggt aatattccgg tagcacttgc actaaatgac
    10321 cactggcaat gttttcgcta acatcccacc aggaacgcag agcaatcccc tgcccatcga
    10381 gacaccactg atggacaatt tctccatgat tagacgacag tgggccggtg actttaatcg
    10441 catggtggcc ttctttattg cgtaattgcc agacaccaaa aggatgatcg cgctctttaa
    10501 tgaccaggca cggcaatgca gaaagatcgg ttaaatgttt cggcgcgcca tgttgcgcaa
    10561 taaattccgg cgacgcgcag agaatgcggt aattggtcgc cagtttgcgg gcgattaaat
    10621 taggggcgat gtcatcgcca atgcgaatat cgagatcgac accttcattg accaaatcga
    10681 ccagccggtc ttccacatca aaacgtaatt ccagttgcgg atacgccttc gccagcgctg
    10741 ataacgccgg agccaccacc tgtcgtccaa aaccaaagct gctgataata cgcagcatcc
    10801 cctgtggcac ctgacgcacg tcagaaagtt cgtccatcat ctgaccgaca tcctgcaaaa
    10861 tccgctgcgc ccattcataa atccgctctc cttcttcggt aatggtgacg cggcgggtgg
    10921 tgcggtgtaa caacaccacg tttagcgttt gctccagcaa ggcgatgcgc ttgctgacga
    10981 acgccggtga aacgcccagt tcttccgcca cggcggcaaa accagcccgg cgagccacca
    11041 gcataaagac gcgcaaatca ttcagcagcg gtaaattatt catgattcgt gttttatgtt
    11101 tcaccagtta cggggattaa ttccttttca gtcaattata ggatggtgat gttgtcaatt
    11161 ttgatggtca ggaagtgaga acccaatgat gaaaacgatg cgtattgctg cgatcccggg
    11221 agacgggatt ggcaaagaag tccttcctga agggattcgc gtgttacagg ctgccgctga
    11281 gcgctggggc ttcgccttga gttttgagca aatggagtgg gcgagctgcg agtattacag
    11341 ccatcacggt aaaatgatgc cggacgactg gcatgagcaa cttagccgtt tcgacgccat
    11401 ctattttggt gccgtcggct ggccggacac cgttccggac catatttcgt tgtggggttc
    11461 gctgctgaaa tttcgtcgtg aattcgatca gtacgtcaac ctgcgcccgg ttcgtctctt
    11521 tcctggcgtt ccctgcccgc tggcgggaaa acagcctggc gacatcgatt tttacgtggt
    11581 cagggaaaac accgaaggcg aatattcctc gctcggcggt agagtgaatg aaggtacaga
    11641 gcatgaagtc gtcattcagg aatcggtatt tacgcgtcgt ggtgtcgatc gcattttgcg
    11701 ttatgccttc gaacttgcgc aaagccgccc acgtaagacg ctaacttctg ccactaaatc
    11761 aaacggttta gccatcagca tgccgtactg ggatgagcga gtggaagcaa tggccgagaa
    11821 ttacccggag atccgctggg acaagcagca tattgatatt ctctgcgcgc gttttgtgat
    11881 gcagccggaa cgcttcgatg tggtggtggc gtccaatttg tttggcgata tcctttccga
    11941 tcttggcccg gcctgcaccg gcaccattgg cattgcccca tccgccaacc tgaatccgga
    12001 acgcactttc ccgtcgctct tcgagcctgt ccacggttcc gcgccggata tctacgggaa
    12061 aaatattgct aaccctatcg ccacaatttg ggccggtgca atgatgctcg attttctcgg
    12121 caatggcgat gagcgtttcc agcaagcaca taacggtatt ctggcagcga ttgaagaagt
    12181 gattgctcac gggccgaaaa caccggatat gaaaggcagt gccaccacac cacaggttgc
    12241 cgacgcgatt tgcaaaatta ttttgcgtta aggtcaaacc agtttatttg aaccgcgtca
    12301 ctgacgcggt ttttttattc gttctttgca gttaataacc tgcgtcattt caccttttat
    12361 tgtttccgtt tcgtgtttta tggctttccg tattcttaat tgtttaattt atgtaacatg
    12421 caaataatca atttgttaag cacaacatac aactcattac cctgcaaata attacaactg
    12481 gcaaaagcca gagacgggct tttatgccca ggagatgact catgatgagc aatgttaaga
    12541 aaaaagatgt gccgctgata agcatcagcc tggtggccat tcttttcatc gcagctgcat
    12601 taagcctttt cccacaacaa tcggccgacg cggccaacgc aatatacact tttgttacgc
    12661 gtacgttagg ttccgccgta caggtattgg ttttgctggc aatgggactg gtgatttatt
    12721 tagccaccag taaatacggc aatattcgtc ttggcgaagg aaaaccggaa tacagcacgc
    12781 tctcctggct gtttatgttt atttgtgccg gtttaggttc ttctacgctt tattgggggg
    12841 ttgctgaatg ggcctattat tatcaaacgc ctggattaaa tatcgcaccg cgttcacaac
    12901 aggcactcga atttagcgtc ccttactcct tcttccactg gggcatcagc gcctgggcaa
    12961 cttatacgct ggcctcatta atcatggctt atcactttca tgtgcggaaa aacaaaggtc
    13021 tgagtctttc cggcattatt gccgcgatta ccggcgttcg cccgcaaggc ccatggggaa
    13081 aactggttga tttgatgttc ctgatcgcca ctgtcggtgc actgactatt tcccttgttg
    13141 tcaccgcagc aacctttact cgtggacttt ccgcactgac cggtttaccc gataacttca
    13201 ccgtgcaggc atttgtgatc ctgctttccg gcggcatttt ttgcctaagc tcatggattg
    13261 gtatcaacaa cggtttgcaa cgtctgagca aaatggttgg ctggggcgcg ttcctgctgc
    13321 cattactggt gctgattgtc ggtccaaccg aatttattac caacagcatc atcaatgcca
    13381 tcggcctgac cacgcaaaac ttcctgcaaa tgagcttatt caccgatccg cttggcgatg
    13441 gttcatttac ccgcaactgg accgtattct actggctgtg gtggatctca tacacccctg
    13501 gcgtagcaat gtttgtcacc cgcgtttccc gcggtcgtaa gattaaagaa gttatctggg
    13561 gactgatcct cggcagcacc gtcggttgct ggttcttctt cggcgtaatg gaaagctatg
    13621 ccattcacca gtttatcaat ggcgtaatca acgtcccaca ggtgctggaa acactgggcg
    13681 gcgagacagc tgtgcagcaa gttctgatgt cgttgccagc cggtaaattg ttcctcgccg
    13741 catacctggg cgtaatgatt attttccttg cctcgcatat ggatgcagtg gcctacacca
    13801 tggcggcgac cagtacgcgt aatctccagg aaggtgacga tcctgaccgt gggctgcgtc
    13861 ttttctggtg cgtggtgatc actctgatcc cgctttccat cttgtttacc ggtgcttcgc
    13921 tggaaacgat gaaaaccacc gtcgtgctca cagcccttcc cttcctcgtc attttactgg
    13981 tgaaagtcgg cggatttatt cgctggctga aacaggatta cgccgacatt ccggctcatc
    14041 aagttgaaca ttatctcccg cagacaccgg ttgaagccct ggaaaaaaca ccagtgctcc
    14101 ctgcgggaac cgtattcaaa ggcgacaact gagcgccatc atcctaacga taaaggtatc
    14161 cctatgagca atctgagccc tgactttgta ctacccgaaa atttttgcgc taacccgcaa
    14221 gaggcgtgga ccattcctgc ccgtttttat accgatcaga acgcgtttga acacgaaaaa
    14281 gagaacgtct tcgccaaaag ctggatttgc gtcgctcaca gcagcgaact ggcgaatgcc
    14341 aatgattatg tgacgcggga gatcattggc gaaagcatcg tgctggtacg cggtcgtgat
    14401 aaggttttgc gcgcgttcta taacgtgtgt ccgcaccgtg gtcatcagtt gttgagcggt
    14461 gaaggaaaag caaaaaatgt gattacctgc ccatatcacg catgggcatt caaactcgat
    14521 ggcaacctgg cccatgcacg taactgcgaa aacgtcgcca atttcgatag cgacaaagcg
    14581 caactggttc cggtgcgtct ggaagaatat gccggattcg tcttcatcaa catggacccc
    14641 aacgccacca gcgtagaaga tcagttaccc ggtctgggcg cgaaagtgct ggaagcctgc
    14701 ccggaagtcc acgatctgaa actggcggcc cgctttacca cccgcacgcc tgccaactgg
    14761 aagaacattg tcgataacta tctcgagtgc tatcactgtg gtccggcgca tccaggtttc
    14821 tccgactccg tacaggttga tcgttactgg cacaccatgc acggtaactg gacgctgcaa
    14881 tacggtttcg ccaaaccgtc cgaacagtcg tttaaatttg aagagggtac ggatgcggca
    14941 ttccacggtt tctggctgtg gccgtgcacg atgctgaacg tcaccccgat caaagggatg
    15001 atgacggtca tttatgaatt cccggtggat tctgaaacta ccctgcaaaa ctacgatatt
    15061 tacttcacca atgaagagtt aaccgacgag caaaaatcgc tgattgagtg gtatcgcgat
    15121 gtgttccgtc cggaagattt acgtctggtt gaaagcgtac agaaagggct gaaatcgcgt
    15181 ggctatcgtg gtcaggggcg catcatggcc gacagtagcg gtagcggcat ttccgaacat
    15241 ggtatcgccc atttccataa tctgctggcg caggtattta aggactaatg acatcggcgg
    15301 cggtattttc cgccgccggg ctgatttttg atggagtaca gcaatgtcag actatcaaat
    15361 gtttgaagta caggtgagtc aggtggaacc gcttaccgaa caggtgaaac gcttcacgct
    15421 ggtggcaacc gatggcaaac cgctgcctgc gttcaccgga ggaagtcaca tcatagtgca
    15481 gatgagcgat ggtgataacc agtacagcaa tgcgtattca ctactgagtt caccgcataa
    15541 cacctctagt tatcagattg ccgttcgact ggaagaaaac tcgcgcggcg gttcccgctt
    15601 tctgcatcag caggtaaaag tgggcgatcg gttaacgatc tcaacgccta ataacctgtt
    15661 tgcgctaatt tcctcagcca gaaagcatct gtttatcgcg ggcggtattg gtatcacccc
    15721 tttcctgtcg cacatggcag agttgcaaca cagcgacgtc gactggcaac tacattactg
    15781 ctcgcgaaat ccagaaagtt gcgcatttcg tgatgagcta gtccagcatc cgcaggctga
    15841 gaaagtccat ttgcatcatt catcaaccgg aacacgactg gaattagcgc gattattggc
    15901 ggatatcgaa cctggcacac acgtttatac ctgtggcccc gaggcgctaa ttgaagcggt
    15961 aagaagtgaa gctgcgcgtc tggacatcgc cgccgatacg ctgcactttg agcaatttgc
    16021 tatcgaagac aaaaccggcg atgcatttac cctggtgctt gcccgttccg gaaaagagtt
    16081 tgtggtgccg gaagagatga ctattttgca ggttattgaa aataataaag ccgcgaaagt
    16141 ggaatgttta tgtcgtgaag gggtatgcgg aacctgcgaa acggcaatac tggaaggtga
    16201 agctgaccat cgggatcagt attttagcga tgaagagcgt gccagccagc aaagtatgtt
    16261 gatctgttgt tcgcgtgcga agggtaaacg cctggtgttg gatttgtagt ttgccgaagc
    16321 cggactgagc gcgcccagtc cggcttcgga agattttact gcggatattc ctgcaataaa
    16381 ttgtgtaatg cttccgccat cagctcacca cgccagccgg aaatcagctc cggcaaattg
    16441 ttctgcggtt tcagtttcca gtgccagttc agcagttggt tgatttgccg acgcgatgcc
    16501 agcaattcgg cgctgatctt gtgcgtttcg ctcacgtcag taatcagcga cttaatcgct
    16561 ttaaacgctt tacgataacc cggcatgtcc atcaggttaa gcatcggctg cggtaaggcc
    16621 tcttccggca atgtctgcgc tttttccacc agcgccagca gcgttttacc gtgaaagcgg
    16681 atttcgctac cggataaacc caggctgtcc agttcgccta aacttcccgg catataacgc
    16741 gctaccgacc acaaatgctc ttcacgcacg acaaagttca ccgccagatc gcgctcacgc
    16801 gccttgcgca gtcgccagtc ggctaacagt tgcagacagg ccagttggcg tgtgcgtaat
    16861 tgccaggcat tggtgatatc acgccaggca tcttccggcg caacgacttc ctgacgacgc
    16921 atttgcatca ggcggcattc atccagcgcc gctggtagcc agccggaggc ctccgtttct
    16981 atcataagct tggcggtgat cggtaacaga taccagacat ccgccgctgc atattcacac
    17041 tgacgttcgg tcagcggtct ggccagccag tcggtgcgcg attcactctt gtccagcgta
    17101 acgccggaat actcttccac catggaagcg aaaccccatg acatcgggcg tccgcagaag
    17161 gcagcaagga tttgcgtgtc aatcaggggt tgtggtaatt cgccaaagac attgaggaac
    17221 acttccagat cttcactgcc tgcatggaga aattttgtga tggacggatc gcgcaggatc
    17281 gctttcagcg gtgaccagtc ggtgatcccg agaggatcaa ttagcgccag atgctcgcca
    17341 tcgaaaagtt gaatcaaccc cagctgcggg taataagtgc gcgtacgaac aaattcagta
    17401 tccagggcta tcgccggaaa ggcacggacg gcttcacaca aagaagccag cgcatcgtcc
    17461 gtggtaatca tttggtaatt caaattgttt tctctttagt gggcgtcaaa aaaaacgccg
    17521 gattaaccgg cgtctgacga ctgacttaac gctcaggctt tattgtccac tttgccgcgc
    17581 gcttcgtcac gtaattctcg tcgcaaaatt tttccgacgt tagatttcgg taactcatca
    17641 cgaaactcca ccagcttcgg tactttgtag cccgtgagct gacggcggca aaaagtcacc
    17701 agtgactctt cggtaagcga tggatctttt ttcactacaa agatttttac cgcttcacca
    17761 ctggagccgg aaggtacgcc aacagccgcg acttcctgta cgccaggatg ctgcatgacg
    17821 acatcttcaa tctcgttggg atagacgtta aaaccggaaa ccagaatcat gtctttttta
    17881 cgatcgacaa tgcgcaggaa cccttcttca tccatcaccg cgatgtcgcc ggtgtgtaac
    17941 cagccatttt tgatgatctc atctgtagca tccggacgct gccagtaacc cagcatcacc
    18001 tgcggtcctt tgacacaaag ctcacccggt tgacccggtg gtacttcatt atcatcatca
    18061 tccaccagtt tggcttctgt tgacggtact ggtaggccga tactgccgct gtggtaatca
    18121 atatcgtacg ggttaacact taccaatggc gcacattcgg ttaagccata accttcaagc
    18181 agatattgtc cggtcagttt cacccaacgt tcagcaacca cttgttgaac aggcatacca
    18241 ccgcctgcgg aaagatgcag actggagaaa tccagctgct ggaactcttt attgttcagc
    18301 aacgcattga acaaggtgtt aacgccagtg atagcggtaa acggatattt cgctaactct
    18361 tttaccaacc ctggaatatc acgcgggtta gttataagca ggttctgccc acccagttcg
    18421 ataaacagca ggcagttaat ggtcagggca aaaatgtgat acagcggcag tgccgttact
    18481 accagttctt tacctggatg cagcaacggc ccataggtgg cgttgacctg ttccaggttc
    18541 gccagcatat tgcggtgagt cagcatcgcg cctttcgcca cacctgtggt gccgccggtg
    18601 tattgcagga aggctaaatc ttccggcacc agttcgggtt tgacgtactg catccggtag
    18661 ccgttatgca gtgcgctacg aaatgaaatg gcatctggca gatggtattt cggcaccaaa
    18721 cgcttgatgt atttaacaac gaaattgact accgtgcctt ttgccgtaga tagctgatcg
    18781 cccatacggg tcagaattac gtgctgaacg gcggttttat caaccacttt ttccagtgtg
    18841 tgagcaaagt tagacacaat aacaatcgcc gatgcgccgc tatcgttaag ctgatgctca
    18901 agctcacgcg gggtatacaa cgggttaacg tttacgacga tcatcccggc acgcaaaatg
    18961 ccaaacagcg ccaccggata ttgcaataaa ttaggcatca tcaacgcaac gcgatcgcct
    19021 ttcttcagcc ccaacccttg ttgcaaataa gcggcaaacg cgcgactgcg ttcttccagc
    19081 ttgcggaagg tcatcacctc ccccatattc acaaacgcag gctgatcggc gtagcgcgcg
    19141 accgactgct caaacatatc taccagagat tgataacggt cagggttgat ctccgtcgga
    19201 acgtccgcgg gataacggtt aagccaaacc ttcttcaatt cttcacctct aaaatgcgtg
    19261 ttcgtcgtca tcgcaacccc aaatgataca catgccgtta acataatatt aactcatcat
    19321 accagcttga taattaccca acgaaaaggt tgcgaagcgc gtcactattt atttttatct
    19381 ttaccgtaag aatgcagaaa cagcggacca gccgctgttt ctttttcttt aaaaacaagc
    19441 aattattcag ttacaactgt ttgtactctc gcggggccgg gattatacca gccccatccg
    19501 ccgtagccat agggccagcc acgaccgcca taaaaccatg gatcaatcgg ctgaggcggc
    19561 ataatcacct gctgggttaa atgccaacgt ttgtaacccg ttacttgcat caccataaat
    19621 ttatagggcg tattgccgat tttgccgtca accgcaccag tgattggccc tactaccgta
    19681 accagttgtc cacggaaatc caccgggtcc agaaaaccgt tcacatcggc ataaatgcga
    19741 ccgcgagaag gttctcccag cgtcggtctg gctccgctgt ccagcggtac ggtagcaatt
    19801 tccaggcggg ttttcccttg ctggttttgt accgcaacca ctttgccacc aaagcgtgcc
    19861 tcctgaccaa cgtacagctg cggcgcactc atcacccgaa ctaaatcttg ttgcggcgtg
    19921 ggactgctgc ctttaatggc gtccggcaca gtgacacaac cgctcagcat tagcgcaaac
    19981 gtacctgcca gtatgccttt gataacattc ttttgaaccg ccatgatgcg actccttttt
    20041 ttctcagggc atactcttaa gattcattct ttgcccggaa gtttcttcca tgcgacgttg
    20101 ttacgtaaat aaaccggttc ggcatgttcc accgccaccg ttttaccctc ggcaaacatc
    20161 tgacaagcaa tcggcagcat atcttctgca gcggggagta acacttcgcc atcgcgcaaa
    20221 accagcccgc tctctttacc gagatccggc caggcttgcc agcccgtccc taccgtcacc
    20281 cattcgccgg aaagctgttg cattcgttca tgaacgagtt cgggtttgag tacggcttca
    20341 gtttcttcac cgtgccagat accgttttca tcacgctgat attcggccca gtaaacttcg
    20401 cccattcgcg cgtcaatggc tgacagcacg cgtgttgcgc cgtttttgcg ccacgcccct
    20461 tgcgccatcg tcattagcgt ggagacgcca atcatcggta attccgcgcc aagcgccagc
    20521 ccttgcgcga tgccaatacc aatgcgcacg ccggtaaagc taccggggcc gcgcccgtaa
    20581 gccagagcgt taatatcagt cagggaagtt ccgctggtgg tcaggatatc ctgcaccatc
    20641 ggtaagattc gttgagtatg ttcacgaggg caaagctcaa aatgagcgtt gacagtaccg
    20701 tcgttccaca gggcgacaga gcaggcctct gtcgcggtat cgatagccag aattcgcatg
    20761 ggtcttcgtg cttagatcaa taaaaaggcg cgcatcatac catactccgt aacaaattac
    20821 ctggaggatg gtatcgcaag gaaacgaacc gcacgggcaa tgtcacgggt gcgtggcgcg
    20881 ggcggcagac tggcgagaaa cgtcgcgcca taagggcgca ttaccagccg attgtcgcaa
    20941 attaccagca caccgcgatc gtcggcgtcg cgaatcagtc gccctacccc ctgcttgaga
    21001 gtaatgaccg catccggcag ctgcacttca tcaaacgggt cgccaccgcg caaacgacaa
    21061 tcttccatgc gcgcttttaa cagtggatca tccggcgagg taaacggcaa tttgtcgata
    21121 attaccaatg acaatgtatc gccacgcacg tccacccctt cccagaaact actggttgcc
    21181 acaagaagcg cgttaccggc gctgacaaat tgctgcaaca gttgcccttt gctggtttcc
    21241 ccctgcaaca aaacgggaag cgtcatggta gcgcggaact gctcggccag atcgcgcatc
    21301 atggcgtgcg aggtacaaag cataaaacaa cgaccgttgt tagcttcgat gatcggtcgc
    21361 agcattgccg ccagttgccg tgcacaaccc ggttgattgg tttgcggcag gttgcgcggc
    21421 acacagagta acgcctggcg gctgtaatca aacgggcttg gcaatagcaa cgactccgcc
    21481 tgttctatcc caagccgcga ggtgaaatga tgcagatcgt cgttcaccga cagcgttgct
    21541 gaggtgaaga tccagctacc gggcttttgc gccattaact ctttgaattt atccgccacg
    21601 ctgagcggcg tgagagccag agtaaaatgg cgcgaagtgc attcgtacca gtagctgtag
    21661 cccggctgat tgatctcttt tagccgcttc aaccgtgtgc gatacaacgt ggcgcgctca
    21721 aatgccgcat ccagcaaggc ggaacgcccc agtgacagtt tcgccacgtc ataacaaagt
    21781 tccagggtgt catcgagcag taaaaatgcc cgctgaattt gcggattagc taacagctca
    21841 cgcaggttac cgcgataacc tggttcaccg agttgcagac gaaaatcctg cgcactctgg
    21901 gcaagacgat cagcgcactt ttgtaactgc tgggtgtctt ttaattcggt gcggtaggct
    21961 atggtgatgt cttttgccag gtcgagcagt tgtcgactgg agagtgactg accaaaatac
    22021 tggctggcaa tgtccggtag ctggtgggct tcgtcgaaga tcatgacgtc cgcttccggg
    22081 atcaactcgc caaatccact ctctttaacc accatatccg ccagaaagag atgatggttt
    22141 accaccacca catcggcgtc catcgctttt ttacgtgctt ttaccacaaa gcaatcttta
    22201 tacatcgggc agtcgctgcc aagacagttg tcgttggtgc tggtgaccag cggccacgcc
    22261 tgtgaatctt ccgccacgct gacgcaggtg ctgatatcac catcgactgt ttgattagac
    22321 caggagcgca gcaggatcac atcgcttaag atttgtaccg gcagttcgcc ccccgccagc
    22381 gcctgctgtt cgagacgctc gaggcagagg tagtttgagc gccctttcag cagcgccacg
    22441 ttgcccgtat atttcaatgc ctttgagact gttggcaaat cgcggctgta gagctgatcc
    22501 tgcaacgctt ttgagccggt cgagataatg actttctttt tcgcccgcag cgcaggagcc
    22561 aggtaagcgt aggttttgcc cgtaccggtt cctgcttcca ccaccagcgg ctggcctttt
    22621 tctatcgcct gggtgacggc taccgccatc tgtcgctgtg gttctcgcgg cttaaagcct
    22681 ggtatcgctt tcgccagctg accgtctggt gcaaaatcgt ccgtcacact accccctgtt
    22741 gatttgaaca gggattatgt caggatgagg gcgctttcgc cagttgaagt ggtgacggcg
    22801 acctcacatt gtggcagtct ttgcagcacg aaaatggaag gataatgagg acaaaatgat
    22861 gactatcgtt cgtatcgatg ctgaagcccg ctggtctgat gtagtaatcc acaacaacac
    22921 gctctactac actggtgtac cggaaaacct cgacgccgat gcctttgagc aaaccgccaa
    22981 cacgctggcg cagattgacg ccgtgctgga aaaacagggc agcaataaat cgagtattct
    23041 ggatgccacc attttcctgg ccgataaaaa cgacttcgcg gcgatgaata aagcgtggga
    23101 tgcctgggtt gtcgcgggtc atgcgccggt gcgctgcacg gtacaagcgg gtttgatgaa
    23161 cccgaagtat aaagttgaaa ttaagattgt ggctgcggtg taagttttat cgaagcaaaa
    23221 taagtcagac gataatttat cgataatact ggtcggtttt acataaatcg accagagaat
    23281 aagatattac
//
LOCUS       DAPGRN010000071        23104 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPGRN010000071 DAPGRN010000000
VERSION     DAPGRN010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.071"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..607
                     /locus_tag="RHW33_002969"
     CDS             53..607
                     /locus_tag="RHW33_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV1646450.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNFAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(617..2044)
                     /locus_tag="RHW33_002970"
     CDS             complement(617..2044)
                     /locus_tag="RHW33_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDV1646451.1"
                     /translation="MKNCWKILNIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWHEYPSTQADALMLQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2041..2748)
                     /locus_tag="RHW33_002971"
     CDS             complement(2041..2748)
                     /locus_tag="RHW33_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV1646452.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDNLPLLREFLARKEYDDSGNDMFYQLFASDDAYYATLPWQP
                     LADYPTCPETLKDMSDL"
     gene            2912..3889
                     /locus_tag="RHW33_002972"
     CDS             2912..3889
                     /locus_tag="RHW33_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDV1646453.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEQGDSEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVAWYQIAAESGMSYAQNNLGWMYRNGNGIAQDYTLAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3959..4441)
                     /locus_tag="RHW33_002973"
     CDS             complement(3959..4441)
                     /locus_tag="RHW33_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV1646454.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4677..7259
                     /gene="leuS"
                     /locus_tag="RHW33_002974"
     CDS             4677..7259
                     /gene="leuS"
                     /locus_tag="RHW33_002974"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV1646455.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPQY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7274..7855
                     /gene="lptE"
                     /locus_tag="RHW33_002975"
     CDS             7274..7855
                     /gene="lptE"
                     /locus_tag="RHW33_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV1646456.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISTKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7855..8886
                     /gene="holA"
                     /locus_tag="RHW33_002976"
     CDS             7855..8886
                     /gene="holA"
                     /locus_tag="RHW33_002976"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV1646457.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANCGVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8888..9529
                     /gene="nadD"
                     /locus_tag="RHW33_002977"
     CDS             8888..9529
                     /gene="nadD"
                     /locus_tag="RHW33_002977"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV1646458.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCDDLLPEPVLTYINQQGLYR"
     gene            9553..10164
                     /locus_tag="RHW33_002978"
     CDS             9553..10164
                     /locus_tag="RHW33_002978"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV1646459.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10424..10741
                     /gene="rsfS"
                     /locus_tag="RHW33_002979"
                     /gene_synonym="rsfA"
     CDS             10424..10741
                     /gene="rsfS"
                     /locus_tag="RHW33_002979"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV1646460.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10745..11212
                     /gene="rlmH"
                     /locus_tag="RHW33_002980"
     CDS             10745..11212
                     /gene="rlmH"
                     /locus_tag="RHW33_002980"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV1646461.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11243..13144
                     /gene="mrdA"
                     /locus_tag="RHW33_002981"
     CDS             11243..13144
                     /gene="mrdA"
                     /locus_tag="RHW33_002981"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV1646462.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13147..14259
                     /gene="mrdB"
                     /locus_tag="RHW33_002982"
                     /gene_synonym="rodA"
     CDS             13147..14259
                     /gene="mrdB"
                     /locus_tag="RHW33_002982"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV1646463.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14270..15358
                     /gene="rlpA"
                     /locus_tag="RHW33_002983"
     CDS             14270..15358
                     /gene="rlpA"
                     /locus_tag="RHW33_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV1646464.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FANKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15498..16709
                     /gene="dacA"
                     /locus_tag="RHW33_002984"
     CDS             15498..16709
                     /gene="dacA"
                     /locus_tag="RHW33_002984"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV1646465.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16819..17082
                     /locus_tag="RHW33_002985"
     CDS             16819..17082
                     /locus_tag="RHW33_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV1646466.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17183..17824
                     /gene="lipB"
                     /locus_tag="RHW33_002986"
     CDS             17183..17824
                     /gene="lipB"
                     /locus_tag="RHW33_002986"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV1646467.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18084..19037
                     /locus_tag="RHW33_002987"
     CDS             18084..19037
                     /locus_tag="RHW33_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV1646468.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRIQEMFPDRQISFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19246..20211
                     /gene="lipA"
                     /locus_tag="RHW33_002988"
     CDS             19246..20211
                     /gene="lipA"
                     /locus_tag="RHW33_002988"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV1646469.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20312..20515)
                     /gene="tatE"
                     /locus_tag="RHW33_002989"
     CDS             complement(20312..20515)
                     /gene="tatE"
                     /locus_tag="RHW33_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV1646470.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20644..21432)
                     /locus_tag="RHW33_002990"
     CDS             complement(20644..21432)
                     /locus_tag="RHW33_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV1646471.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILALPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21525..21908
                     /gene="crcB"
                     /locus_tag="RHW33_002991"
     CDS             21525..21908
                     /gene="crcB"
                     /locus_tag="RHW33_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV1646472.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21962..22171)
                     /gene="cspE"
                     /locus_tag="RHW33_002992"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21962..22171)
                     /gene="cspE"
                     /locus_tag="RHW33_002992"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV1646473.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22346..22906)
                     /gene="pagP"
                     /locus_tag="RHW33_002993"
     CDS             complement(22346..22906)
                     /gene="pagP"
                     /locus_tag="RHW33_002993"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV1646474.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5954 a         5787 c         5688 g         5675 t
ORIGIN      
        1 tgagtataaa cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa
       61 aaaattaatg tatatattcg caatttttat agttgcagca attacctgta ttagccaacc
      121 caagaaaacg acgttgcgtg ataaagccat ggtgaatttt gcctttgatt atttaagctc
      181 accgggcagt cttccattca ccacggcagc cacggagctt tccgcgattc atggtcactc
      241 aacgtcgcaa tatcgccttg gagaatttta tcttcatggt agcgacggta aaccactgga
      301 ttatacacag gcgagatact ggtatgagca atcagcggaa caggaaaatc cacgcgcgca
      361 aagtaaactg gggtggatct acctcaaagg tctgggggtc aaacccgaca cccgtaaagc
      421 aattctctgg tataaggaag cagctgaaca agggtatgct catgctcaat atactttagg
      481 tttgatctac agaaatggct caggtattaa tgttaaccat tatgaatctc aaaaatggtt
      541 aaaactggcc gccaaacaac attacaaaaa tgcggaaaga ttacttgccg ggcttcccgc
      601 acattaacgt cgaatgtcac ccgaacagat ggagtatttt ccctatcaaa ccagcaaaga
      661 taaaaatata gaaaataatt ttaatgatgc ccagacagcc tggcccacca tcgtcaacgg
      721 gcattggctg cggttgcggc tccggttcaa cagtctgctt ctcggtaaaa ttcgccgcct
      781 gaattaataa tcgacgaata attaatgctt ccagtggaca acgattttct gcatcaagac
      841 ggcttacgat cggcattaat gattcttctg ttggttggct ggcctgcact aattgtttgg
      901 caactaaata taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct
      961 tacgtccttt gtgcaaaacc atctcgacca gtgaacgccc cagagcagag agtttttcat
     1021 cactgagtat ttcgtataaa tgaaaacgcg tacgagcgct gtcgccgggg acataaagta
     1081 gcggttttcc ctggtcgtta accagttcgc gagcttcaat catcatcacc actaatgggt
     1141 aataatcgac tcgatgctga cggcaccatt gcaacattaa agcatccgcc tgagtggagg
     1201 gatattcgtg ccagtagtca caaagttctg ccagcaggct ttcgccttct ccacaataga
     1261 cgcgttgcgc gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg
     1321 accgggcgta atccaggagt tcgggaattc ccaggcgacc tgcgctatac cagcggagta
     1381 aatttctgtg cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca
     1441 atgtcagcca ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg
     1501 tgtcattttg cacggcaacg ggcagatgca gaatggtctg gaagttaaaa acattcccct
     1561 tcgcaatggc atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct
     1621 cctgcaacca ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag
     1681 tgttcatggc gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat
     1741 aatttaattg ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg
     1801 caagtaacgc acgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt
     1861 gccaaacaga tttagcaggc gactgcgcaa tccggagcgc ttcctcatac gcctgacgaa
     1921 gttgtttaaa accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag
     1981 cgcggcggat aatatcgacg tcagtcgttt cctctatgtt gaggatcttc cagcaatttt
     2041 tcataggtcg ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg
     2101 ccagggtaag gtcgcgtaat atgcatcgtc actggcaaat aattgataaa acatatcatt
     2161 gccggaatcg tcgtattctt tacgcgctaa gaattcacgt aataaaggca gattatcatc
     2221 ttcgggagac tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt
     2281 actataataa cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc
     2341 actcaaccag ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca
     2401 tgctttaatt cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc
     2461 ctgaccttgc tcatttaatt cagcaagata gtcacgccat tcacatggag aataacgaaa
     2521 ccagcggtga taaaaccctg ccaaccgcgc ggcatgaccg ttatcgatca ttcggtgaat
     2581 tgtgcgcagc aggtcatcac ggtcatcaat tccccaggag gaagaaaggt caaaaaagca
     2641 tgtacctaaa tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc
     2701 catcggagtg gaaagtgcaa acaatatttt ttgcgactcc atgtccatat aatttatcct
     2761 taaacattca tttaatgaat gtaaactaca ttacgaggct catttattta tacttttctg
     2821 acacaacata atacgcataa aataagactt acgcaaaaac ggaacacata aaaatataat
     2881 atgctttatc tgcaaccata aggaaatgat aatgattttt acgtcaagtt gctgcgataa
     2941 tttgtcaata gatgagatta tcgaacgtgc tgaacaaggc gatagtgagg ctcaatatat
     3001 tgttgggttt tattataatc gcgatagcgc aattgactct ccggacgacg aaaaggcctt
     3061 ttactggctg aagctggccg ctgagcaagg tcattgtgaa gcacagtatt ccttagggca
     3121 aaagtatacc gaggataaaa gccgtcataa agataatgag caagccatct tctggctgaa
     3181 aaaagctgcc ctacaaggcc atactttcgc ttccaacgcc cttggctgga tactggatcg
     3241 tggagaagac cccaactata aagaagcggt tgcctggtat cagatagccg cggagagcgg
     3301 aatgtcttat gcgcaaaata atcttgggtg gatgtacaga aatggcaacg gaatcgcaca
     3361 agactatacg ctggcatttt tctggtacaa acaagctgca ttacaaggcc atagtgacgc
     3421 gcaaaacaat ctggccgatc tttatgaaga cggaaaaggc gttgctcaaa acgagacact
     3481 cgccgcattc tggtatttga aaagcgcaca gcagggtaat cggcatgctc agtttcaaat
     3541 tgcatgggat tataacgctg gcgaaggggt ggaccaggac tataagcaag cgatgtactg
     3601 gtatctgaag gctgccgctc aggggagcgt cggcgcttac gtcaacatcg gttatatgta
     3661 taaacacgga caaggcgttg agaaagatta tcaggctgcc tttgaatggt ttacgaaagc
     3721 cgctgaatgc aatgacgcca ctgcctggta taacctggcc attatgtatc actacggaga
     3781 aggaagacct gtcgatctcc gacaggctct cgacctgtat cgtaaagttc agtcatccgg
     3841 aaccagagat gtcagtcagg aaattcgtga gactgaagat ttactgtaga gataaaaaca
     3901 agggccgggg aatttcattc ggcccatatt ctctttattg ctgtgcgaaa tcagactatt
     3961 acggctcaaa cgggcgtctc tggaactggt catgaccaca tttcgggcat agcgtcagca
     4021 cttccggtgt gtagatcggg agatggaagt gacatttctc gcagaccaga tttcccagcc
     4081 cgaccacttc tccgctgtga taaaccccat gatgattgag gtcctggaaa acttcgcgcc
     4141 attcaagctg cgttttatcg gtgatgtctg ccagctcctg ccacaagctt tctttaatca
     4201 cccgcataaa gacgctgtca gattcttctt tcaggctctc ttcatagctc atggcgaact
     4261 cttccaggtc acgtctgaca gctcgcgtca actcatcgac ctcggttcgc gttaactccc
     4321 ctgtttttat tacgcgctcg cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat
     4381 tgcgcaggcg ttcgctcagt gacgcaacca gttcacggta atattgagca accttgttca
     4441 tcgtttcatc tcccgggtcg ttaactgact ataataatag acgttctttg ttccttgctt
     4501 tgctaatacg cccacaaact ctgtaaatta atgtatgaat aatccgggca tcatttcaac
     4561 gtcgagtgcg cgaaaggctg ttttgacgcg gacgtttggg ctatgctatg cggatctgaa
     4621 aaaccacctc taacgctact tttgtagccg taatgaaaac aggaccactg gctgccatgc
     4681 aagagcaata ccgcccggaa gagatagaat ccaaagtaca gcttcattgg gatgagaagc
     4741 gcacatttga agtaaccgaa gacgagagca aagagaagta ttactgcctg tctatgcttc
     4801 cctatccttc tggtcgacta cacatgggcc acgtacgtaa ctacaccatc ggtgacgtga
     4861 tcgcccgtta ccagcgcatg ctgggcaaaa acgttctgca gccgatcggc tgggacgcgt
     4921 ttggtctgcc tgcggaaggc gcggcggtga aaaacaacac cgctccggca ccgtggacgt
     4981 acgacaacat cgcgtatatg aaaaaccagc tcaaaatgct gggctttggt tatgactgga
     5041 gccgcgagct ggcaacctgt acgccggaat actaccgttg ggaacagaaa ttcttcaccg
     5101 agctgtataa aaaaggcctg gtatataaga agacttctgc ggtcaactgg tgcccgaacg
     5161 accagaccgt actggcgaac gaacaagtta tcgacggctg ctgctggcgc tgcgatacca
     5221 aagttgaacg taaagagatc ccgcagtggt ttatcaaaat cactgcttac gctgacgagc
     5281 tgctcaacga tctggataaa ctggatcact ggccagacac cgttaaaacc atgcagcgta
     5341 actggatcgg tcgttccgaa ggcgtggaaa tcaccttcaa cgttaacgac tatgacaaca
     5401 cgctgaccgt ttacactacc cgcccggaca cctttatggg ttgtacctac ctggcggtag
     5461 ctgcgggtca tccgctggcg cagaaagcgg cggaaaataa tcctgaactg gcggccttta
     5521 ttgacgaatg ccgtaacacc aaagttgccg aagctgaaat ggcgacgatg gagaaaaaag
     5581 gcgtcgatac tggctttaaa gctgttcacc cattaacggg cgaagaaatt cccgtttggg
     5641 cagcaaactt cgtattgatg gagtacggca cgggcgcagt tatggcggtt ccggggcacg
     5701 accagcgcga ctacgagttt gcctctaaat acggcctgaa catcaaaccg gttatcctgg
     5761 cagctgacgg ctctgagcca gatctttctc agcaagccct gactgaaaaa ggcgtgctgt
     5821 tcaactctgg cgagttcaac ggtcttgacc atgaagcggc cttcaacgcc atcgccgata
     5881 aactgactgc gatgggcgtt ggcgagcgta aagtgaacta ccgcctgcgc gactggggtg
     5941 tttcccgtca gcgttactgg ggcgcgccga ttccgatggt gacgctggaa gacggtaccg
     6001 taatgccgac cccggacgac cagctgccgg tgatcctgcc ggaagatgtg gtaatggacg
     6061 gcattaccag cccgattaaa gcagatccgg agtgggcgaa aactaccgtt aacggtatgc
     6121 cagcactgcg tgaaaccgac actttcgaca cctttatgga gtcctcctgg tactatgcgc
     6181 gctacacttg cccgcagtac aaagaaggta tgctggattc cgaagcggct aactactggc
     6241 tgccggtgga tatctacatt ggtggtatcg aacacgccat tatgcacctg ctctacttcc
     6301 gcttcttcca caaactgatg cgtgatgcag gcatggtgaa ctctgacgaa ccagcgaaac
     6361 agttgctgtg tcagggtatg gtgctggcag atgccttcta ctatgttggc gaaaacggcg
     6421 aacgtaactg ggtttccccg gttgatgcta tcgttgaacg tgacgagaaa ggccgtatcg
     6481 tgaaagcgaa agatgcggca ggccatgaac tggtttatac cggcatgagc aaaatgtcca
     6541 agtcgaagaa caacggtatc gacccgcagg tgatggttga acgttacggc gcggacaccg
     6601 ttcgtctgtt tatgatgttt gcttctccgg ctgatatgac tctcgaatgg caggaatccg
     6661 gcgtggaagg ggctaaccgc ttcctgaaac gtgtctggaa actggtgtac gagcatactg
     6721 cgaaaggtga tgttgcggca ctgaacgttg atgcactgac tgaagatcag aaagcgctgc
     6781 gtcgcgatgt gcataaaact atcgctaaag tgaccgatga tatcggccgt cgtcagacct
     6841 tcaacaccgc aattgcggcg attatggagc tgatgaacaa actggcgaaa gcaccgaccg
     6901 atggcgagca ggatcgcgct ctgatgcagg aagcgctgct ggccgttgtc cgtatgctta
     6961 acccgttcac cccgcacatc tgcttcacgc tgtggcagga actgaaaggc gaaggcgata
     7021 tcgacaacgc gccgtggccg gttgctgacg aaaaagcgat ggtggaagac tccacgctgg
     7081 tcgtggtgca ggttaacggt aaagtccgtg ctaaaatcac cgttccggtg gacgcaacgg
     7141 aagaacaggt tcgcgaacgt gctggccagg aacatctggt agcaaaatat cttgatggcg
     7201 ttactgtacg taaagtgatt tacgtaccag gtaaactcct caatctggtc gttggctaag
     7261 cgcgggagga agcgtgcgat atctggcaac attgttgtta tctctggcgg tgttaatcac
     7321 cgccgggtgt ggctggcatc tgcgtgacac cacgcaggtt ccttccacta tgaaggtcat
     7381 gatcctggac tcaggcgatc cgaacgggcc attaagccgt gcggtgcgta accagttacg
     7441 tctgaatggt gtcgagttgc ttgataaaga aaccacgcgt aaggacgttc catccttgcg
     7501 tttgggtaaa gtgagcatcg cgaaagatac cgcatcggta ttccgtaacg gtcaaacagc
     7561 agagtatcag atgatcatga cggtcaatgc gaccgtgttg atccccggcc gtgacatcta
     7621 cccgattagc accaaggtct tccgttcgtt cttcgataac ccgcaaatgg cgttagcgaa
     7681 agataacgaa caagacatga tcgtaaaaga gatgtacgac cgtgctgccg aacagctgat
     7741 tcgtaagctg ccaagcatcc gtgctgcgga tattcgttcc gacgaagaac agacgtcgac
     7801 tacaacggat actccggcaa cgcctgcacg cgtctccacc acgctgggta actgatgatt
     7861 cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag ggctgcgcgc ggcgtatctt
     7921 ttacttggta acgatcctct gttattgcag gaaagccagg acgctgttcg tcaggtagct
     7981 gcagcacaag gattcgaaga acaccacact ttttccattg atcccaacac tgactggaat
     8041 gcgatctttt cgttatgcca ggccatgagt ctgtttgcca gtcgacaaac gctattgctg
     8101 ttgttaccag aaaacggacc gaatgcggcg atcaatgaac aacttctcac actcaccgga
     8161 cttctgcatg acgacctgct gttgatcgtc cgcggtaata aattaagcaa agcgcaagaa
     8221 aatgccgcct ggtttactgc tcttgcgaat tgcggcgtgc aggtgacctg tcagacaccg
     8281 gagcaggctc agcttccccg ctgggttgct gcgcgcgcaa aacagctcaa cttagaactg
     8341 gatgacgctg caaatcaggt gctctgctac tgttatgaag gtaacctgct ggcgctggct
     8401 caggcactgg agcgtttatc gctgctctgg ccagacggca aactgacatt accgcgcgtt
     8461 gaacaggcgg tgaatgatgc cgcgcatttc accccttttc attgggttga tgctttgttg
     8521 atgggaaaaa gtaagcgcgc gttgcatatt cttcagcaac tgcgtctgga aggcagcgag
     8581 ccggttattt tgttgcgcac attacaacgt gaactgttgt tactggttaa cctgaaacgc
     8641 cagtctgccc atacgccact gcgtgcgttg tttgataagc atcgagtatg gcagaaccgc
     8701 cggggcatga tgggcgaggc gttaaatcgc ctaagtcagc cgcagttacg tcaggccgtg
     8761 caactcctga cacgaacgga actcaccctc aaacaagatt acggtcagtc agtgtgggca
     8821 gagctggaag ggttatctct tctgttgtgc cataaacccc tggcggacgt atttatcgac
     8881 ggttgatatg aaatctttac aggctctgtt tggcggcacc tttgatccgg tgcactatgg
     8941 tcatctaaaa cccgtggaaa cgctggcgaa tttgattggt ctgacgcggg tcacaataat
     9001 ccctaataat gttcctccgc atcgtcccca gccggaagcg aacagcgtgc agcgtaaaca
     9061 catgcttgaa ctggcgattg ccgacaagcc attatttact cttgatgaac gcgagctaaa
     9121 gcgcaatgcc ccctcttaca ctgcgcaaac actgaaagag tggcggcagg aacaaggacc
     9181 ggacgtgccg ctggcgttta ttattggtca ggattcattg ctgacctttc cgacctggta
     9241 cgaatacgaa acgatactcg acaatgcaca tttgatcgtc tgtcggcgtc caggttaccc
     9301 acttgaaatg gcgcaaccgc aataccagca atggctggaa gatcatttga cacataaccc
     9361 ggaagatctt caccttcagc ctgccggtaa aatttatctg gctgaaactc cgtggtttaa
     9421 catctcggcg accatcatcc gcgaacgttt gcaaaacggt gaatcatgtg atgatttatt
     9481 gccggaaccg gtactgactt acattaacca acaaggcttg tatcgctgat acctgtcgtt
     9541 acggagggca tgatgcgact gtggttaatt cgtcatggcg aaacacaagc gaatgtcgat
     9601 ggtctgtaca gcggtcatgc gcccacaccc ctgaccgcac gcggtattga gcaagcgcaa
     9661 aatctgcata cactgctaga tgatgtttcc tttgacctgg ttttatgcag tgaactggaa
     9721 cgggcacagc ataccgcgcg actggttctc agtgaccgcc agctccccgt tcatatcatc
     9781 cctgaactca acgaaatgtt ttttggcgac tgggagatgc gacatcatcg cgacctcatg
     9841 caagaagatg ccgaaaacta tagcgcgtgg tgcaatgact ggcagcatgc tatccccacg
     9901 aacggtgaag gatttcaggc attttcgcaa cgtgtggaac gctttatcgc aaggcttagt
     9961 gaatatcagc actatcagaa tattttaatc gtcagccatc agggcgtact gagtctgtta
    10021 attgctcgtt taattggcat gcctgccgag tctatgtggc attttcgcgt tgaccagggg
    10081 tgttggagcg ccattgatat caaccaaaaa ttcgcgacac tacgcgtcct caatagccgt
    10141 gccatcgggg tcgagaatgc atgacttttc tgttttttta cgggtaagcc gcaacgacca
    10201 ttgacagccc cgggcaggct gatattctcc gcagccagac tttttccgcc agacacgact
    10261 ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg gcgcggggtg ggatgatagc
    10321 ccactttcga aagccgattc ggcgacaatt gtcccgaaat cgcctctggt tcaggtatac
    10381 tgacagacca tttttatcta tttgattcac ccagggggaa aacttgcagg gtaaagcact
    10441 ccaggatttt gttatcgaca aaattgatga cctcaaaggt caggacatca tcgccttaga
    10501 cgttcagggc aaatccagca tcaccgactg catgatcatc tgtacgggta cgtccagccg
    10561 tcatgttatg tccattgctg accacgttgt gcaggagtct cgcgcagcgg gcctgttacc
    10621 gctcggcgta gaaggtgaaa acagcgccga ctggattgtc gtggatttgg gcgatgtgat
    10681 tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa ctggaaaaac tctggagtta
    10741 atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg ccggactggg tacaaaccgg
    10801 ttttaccgag tacctgcgtc gttttccgaa agatatgccc ttcgagctga ttgaaattcc
    10861 ggccggaaaa cgcggcaaga atgcggacat caagcgcata ctcgacaaag agggtgagca
    10921 gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc gatattccag gcaagccctg
    10981 ggatacgccg cagttagccg ctgagctgga acgctggaag ctggatggtc gcgacgtcag
    11041 tctactgatt ggcgggcctg aagggttgtc gcctgcctgt aaagcggcgg ctgagcagag
    11101 ctggtcgctg tcggcgctta ccctccccca tccgctggtt cgcgtgctgg tcgcagagag
    11161 tctgtaccgg gcgtggagca tcaccaccaa ccatccttat caccgtgagt gataagggag
    11221 ctttgagtag aaaacgcagc ggatgaaact acagaactct tttcgcgact atacggcaga
    11281 gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg gggattttgc tgctgaccgg
    11341 cgtgcttatc gccaacctgt ataatctgca aattgttcgc tttaccgact accagacccg
    11401 ctctaatgaa aaccgcatta agctggtgcc tatcgcaccc agccgcggca ttatctatga
    11461 ccgtaatggt atccctctgg ctctcaaccg cactatctac cagatagaaa tgatgccgga
    11521 gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc agcgtggtag atctgaccga
    11581 tgacgatatt gctgcattcc gaaaagagcg cgcacgttca caccgtttca cctctattcc
    11641 ggtgaaaact aacctgaccg aagtacaagt agctcgcttt gccgtcaatc agtaccgttt
    11701 tccgggtgtc gaagttaaag gctataaacg tcgttactat ccttacggtt cggcgttgac
    11761 ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac gtcgaacgcc tgaataatga
    11821 cggcaaactg gccaactatg cggcaacgca tgatatcggt aagctgggca ttgagcgtta
    11881 ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag gttgaagtta acaaccgtgg
    11941 gcgtgttatc cgccagttaa aagaagtccc accgcaagcc ggacacgata tttacctgac
    12001 gctggatctc aaactccagc aatatattga aacgctgctg gcgggtagcc gcgcagctgt
    12061 ggtagtcacc gatccgcgta caggtggggt gctggcactg gtttccacgc ctagttatga
    12121 cccaaacctg tttgttgacg gtatctccag caaagattat tccgccttgc tgaacgaccc
    12181 gaatacaccg ctggtgaacc gcgccacaca gggggtttat cctcccgcgt ctacagttaa
    12241 accctatgtg gcggtttcgg cattgagcgc cggggtgatc acgcgcaata cgacgctgtt
    12301 tgacccaggc tggtggcaac tgccaggttc ggaaaaacgt tatcgtgact ggaaaaaatg
    12361 gggccacggg cgtctgaatg tcacaagatc gctggaagaa tctgcggata ccttcttcta
    12421 tcaggtggct tacgatatgg ggatcgatcg cctctccgaa tggatgggta aattcggtta
    12481 tggtcattat accggtatcg acctggcgga agaacgttcc ggcaacatgc ctacccgcga
    12541 atggaaacag aaacgcttta aaaaaccgtg gtatcagggt gacaccattc cggttggtat
    12601 cggtcagggt tactggacag cgaccccaat ccagatgagt aaggcactga tgatcctgat
    12661 taatgacggt atcgtgaagg ttccgcattt gctgatgagc accgccgaag acggcaaaca
    12721 ggtgccatgg gtacaaccgc atgaaccgcc cgtcggcgat attcattccg gttactggga
    12781 gctggcgaaa gacggtatgt acggtgttgc taaccgccct aacggtacgg cgcataaata
    12841 ctttgccagc gcaccgtaca aaattgcggc gaaatctggt accgctcagg tcttcggtct
    12901 gaaagcgaac gaaacctata atgcgcacaa aattgccgag cgtttacgtg accacaaact
    12961 gatgaccgcc tttgcgcctt acaacaatcc gcaagtggct gtcgccatga ttctggagaa
    13021 cggcggcgcg ggtccggcgg ttggtacgct gatgcgccag atcctcgacc acattatgct
    13081 gggtgataac aacaccgatc tgcctgcgga aaatccagcg gttgccgcag cggaggacca
    13141 ttaatcatga cggataatcc gaataaaaaa acattctggg ataaagtcca tctcgatccc
    13201 acaatgctgc tgattttact ggcattgctg gtttacagcg ccctggttat ctggagcgcc
    13261 agcggtcagg atattggcat gatggagcgt aaaatcggcc aaatcgcgat gggtctggtc
    13321 atcatggtgg tgatggcgca aattcctcca cgcgtttatg aaggctgggc cccctatctc
    13381 tatatcatct gtattatttt gctggtggcg gtagatgctt tcggtgccat ctctaaaggc
    13441 gctcaacgct ggctggacct cggtattgtt cgttttcagc cgtcggaaat tgccaaaata
    13501 gccgtaccac tgatggttgc gcgctttatc aaccgcgacg tttgcccgcc atcgttgaag
    13561 aacactggca tcgcgctggt gctgatattt atgcccacgc tgctggtggc tgcacagcct
    13621 gacctgggaa catcaatcct cgttgcgctt tccggtctgt ttgtactgtt cctctctggc
    13681 cttagctggc gtctgattgg cgtcgcagta gtgctggtgg cggcgttcat tccgattctg
    13741 tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa tgatgctcct ggacccggaa
    13801 tcagacccac tcggcgcggg ctatcacatt attcagtcta aaattgctat tggctccggc
    13861 ggattacgcg gcaaaggctg gctgcatggc actcagtcac agcttgaatt tctccccgaa
    13921 cgccataccg actttatctt cgcggtactg gcggaagagc tgggattagt gggcattttg
    13981 attctgctcg ctctctacat tctgctgatc atgcgcgggc tgtggatagc cgccagagcg
    14041 caaaccacct ttggtcgcgt catggctggt ggcttaatgc tgatattatt cgtttatgtc
    14101 ttcgtaaata ttggtatggt aagcggtatt ctgccggttg taggggttcc gctcccactg
    14161 gtcagttatg gaggatcggc gctaattgtg ctgatggctg ggttcgggat tgtaatgtca
    14221 atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag aggtgcgcaa tgcgtaagca
    14281 gtggctcggg atctgcatcg cggcaggaat gctcgcggca tgtacaagcg atgatggtca
    14341 gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac ggccccatag ttgaaattag
    14401 cggggcggac ccgcgtttcg aaccactgaa cgcgacggca aatcaggatt accagcgcga
    14461 cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt agccaggcgg gactggcggc
    14521 aatctatgat gccgaaccag gcagtaacct gacggcctct ggcgaagctt tcgatccgac
    14581 acagctgacg gcggcccatc caacgcttcc gatccccagc tacgccagaa tcactaacct
    14641 ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc ggtccttacg gcaacgaccg
    14701 cgttatttcg ctttctcgcg cggcagctga ccgtcttaac acgtcaaaca acaccaaagt
    14761 tcgtatcgat ccgattattg tcgcccagga tggttcgctt tctggtcctg gtatggcttg
    14821 taccacagtc gccaaacaga cttacgccct gcctgcacct cccgatttaa gcggcggcgc
    14881 aggaacaagt tcagtgtctg gcccgcaggg tgacattctt ccggtcagta attcgacgct
    14941 aaaaagcgaa gatccgaccg gcgcgccggt aaccagcagc ggtttcctcg gcgcaccaac
    15001 gaccttagcg ccaggtgtac tggaaggcag cgaaccgacg cctgctccac agcccgttgt
    15061 tacagctccg tcgacaacgc ctgcaacctc gcctgcaatg gtgacaccgc aagccgcctc
    15121 gcaaagcgcc agcggcaact ttatggtgca ggtcggggcc gttagcgatc aggctcgtgc
    15181 gcaacagtac caacagcaac tgggacagaa gttcggcgtc cccggtcgcg taactcaaaa
    15241 tggcgcagtc tggcggatcc agcttggccc attcgccaac aaagccgaag ccagtacctt
    15301 gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt attaccaccg cgcagtagcg
    15361 taaagcaggc atctgatgtg tcaatttctg taaatgacgt taacaaagtc acgcggaaag
    15421 tcagatgcct gccggtagtg catttgctat agtagggcac tttttttaat tccatcacgg
    15481 atgtcgtagt tcagaccatg aataccattt tttccgctcg tatcatgaag cgcctggcgc
    15541 tcaccacggc tctttgcaca gcctttatct ctgctgcaca tgccgatgac ctgaatatca
    15601 aaactatgat cccgggtgta ccgcagatcg atgcggagtc ctacatcctg attgactata
    15661 actccggcaa agtgctcgcc gaacagaacg cagatgtccg ccgcgatcct gccagcctga
    15721 ccaaaatgat gaccagttac gttatcggcc aggcaatgaa agccggtaaa tttaaagaaa
    15781 ctgatttagt cactattggc aacgacgcat gggccaccgg taacccggtg tttaaaggtt
    15841 cttcgctgat gttcctcaaa ccgggcatgc aggttccggt ttctcagctg atccgcggta
    15901 ttaacctgca atcgggtaac gatgcttgtg tcgccatggc cgattttgcc gctggtagcc
    15961 aggacgcttt tgttggcttg atgaacagct acgttaacgc cctgggcctg aaaaataccc
    16021 acttccagac ggtacatggt ctggatgctg atggtcagta cagctccgcg cgcgatatgg
    16081 cgctgatcgg ccaggcattg atccgtgacg taccgaatga atactcgatc tataaagaaa
    16141 aagaatttac gtttaacggt attcgccagc tgaaccgtaa cggcctgtta tgggataaca
    16201 gcctgaatgt cgacggcatc aaaaccggac acactgacaa agcaggttac aaccttgttg
    16261 cttctgcgac tgaaggccag atgcgcttga tctctgcggt gatgggcgga cgtactttta
    16321 aaggccgtga agccgaaagt aaaaaactgc taacctgggg cttccgtttc ttcgaaaccg
    16381 ttaacccact gaaagtaggt aaagagttcg cctctgaacc ggtttggttt ggtgattctg
    16441 atcgcgcttc gttaggggtt gataaagacg tgtacctgac cattccgcgt ggtcgcatga
    16501 aagatctgaa agccagctat gtgctgaaca gcagtgaatt gcatgcgccg ctgcaaaaga
    16561 atcaggtcgt aggaactatc aacttccagc ttgatggcaa aacaatcgaa caacgcccgc
    16621 tggttgtgtt gcaagaaatc ccggaaggta acttcttcgg caaaatcatt gattacatta
    16681 aattaatgtt ccatcactgg tttggttaaa aattgaacac ttgaaagtgt aatttccgtc
    16741 cccatatact aagcatcagt aaaaaactcc cgccttctgg cgggagttgc tatttaatta
    16801 cgttacgccg gagctgacat gaaaaccaaa cttaacgaac tgcttgaatt ccctactcct
    16861 tttacttaca aagttatggg gcaggcgtta cctgagctgg ttgatcaggt ggttgaagtg
    16921 gtacagcgcc atgcgccagg tgactacacc ccaacggtaa aaccaagcag caaaggcaac
    16981 taccactcgg tatctatcac tatcaacgcc actcatatcg agcaggttga aacactgtat
    17041 gaagaactgg gcaaaatcga tattgtccgc atggttctgt aactcgcttc tccgttaccc
    17101 ggcccctggt cgggtaactc ccctctcctc gctgtgatat acttttcccc cacttttact
    17161 cattctccac ggagatgccg ttttgtatca ggataaaatt cttgtccgcc agctcggtct
    17221 tcagccttac gagccaatct cccaggctat gcatgaattc accgataccc gcgatgatag
    17281 cacccttgat gaaatctggc tggtcgagca ctatccggta ttcacccaag ggcaggcagg
    17341 aaaggcggag cacattttaa tgcccggtga tattccggtg attcagagcg atcgcggtgg
    17401 gcaggtgact tatcacgggc cggggcaaca ggtgatgtat gtgttgctta acctgaaacg
    17461 ccgtaaactc ggtgtgcgtg aactagtgac cttgcttgag caaacagtgg tgaataccct
    17521 ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg cctggtgtct atgtagggga
    17581 aaagaaaatt tgctctctgg gtttacgaat tcgacgcggt tgttcattcc acggtctggc
    17641 attaaacgtc aatatggatc tttcaccatt tttacgtatt aatccttgtg ggtatgccgg
    17701 aatggaaatg gctaaaatat cacaatggaa acccgaagcg acgactaata atattgcacc
    17761 acgtttactg gaaaatattt tagcgctact aaacaatcct gacttcgaat atattaccgc
    17821 ttaatttcat acctaaatgg cccaatttac actgggtcat tactcataac acctgctaat
    17881 gctttacatc tccttcgcgt ttgtatattt tctttatcac ttgcagaaat gcgatgctac
    17941 gccttgctcc gtaactcacc ccattgatta acttattgaa atagttgatt atggtgagca
    18001 aaagcgttcc gttccaatta gacataagat tattgtaatt tttttaaata ttatttttcc
    18061 atgaataaaa ttggagtaag tgcgtggata gtaataatca aatcgaaccc tgtttaagca
    18121 gaaagtcgtc agaaggtaaa ccacaaatat ttacaacctt acgaaatatt gatcttaacc
    18181 ttctgactat ttttgaagct gtatatgtac ataaagggat cgttaatgca gcgaaagtgc
    18241 ttaatctgac cccctcggca atcagtcagt ctatacagaa actgcgcgtt atattccctg
    18301 acccattgtt tattcgcaaa ggccagggtg tcactcctac cgcatttgcg atgcatctac
    18361 atgagtatat cagtcagggc cttgaatcca ttcttggcgc gctggatatc gaaggaagct
    18421 atgataagca acgaacgata actatcgcga ccaccccctc ggtcggagcc ctggtccttc
    18481 ctgtcattta tcgggcgatt aaaactcact atccgcagct tttactgcgc aacccgccca
    18541 tcagcgacgc ggaaaaccaa ctcagtcagt ttcaaaccga tctcatcatc gataacatgt
    18601 tttgcaccaa tcgtacggtg caacatcatg ttctgttcac cgacaatatg gtgttaattt
    18661 gccgcgaggg aaatccacta ctctctttag aagatgacag agagactatc gacaacgctg
    18721 cacatgtact cctgttaccg gaaggacaaa atttcagcgg tctgcggcag agaattcaag
    18781 agatgtttcc ggaccggcag atcagcttca ccagctacaa cattttgaca atcgctgcac
    18841 tggttgccaa cagtgacatg ttagcgatta ttcccagccg tttttataat ctgtttagcc
    18901 gttgctggcc gctggaaaaa ctgccttttc cgtccttaaa tgaggagcaa atagatttct
    18961 ctatccacta caacaaattc agcctgcgtg atcccatcct gcacggagta atagatgtta
    19021 tccgtaatgc attttgaaaa agtattgtta cagccagatc acagcgcaga acgataagat
    19081 ggcacaatcc aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt
    19141 tcaatgatat actgccagtc gttaattcaa aaatagttga taattacaac aatctattga
    19201 attgaaacgc tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt
    19261 gtgatggaac gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa
    19321 aacgtggcaa cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt
    19381 ccagcggact ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat
    19441 tctgtctgcg aggaagcctc ctgccctaac ctggcggaat gcttcaacca cggcacagca
    19501 acgtttatga tcctcggcgc tatttgtacc cgccgttgtc cgttctgtga cgttgcccac
    19561 ggtcgcccgg tagctcctga tgccaatgaa ccagtgaaac tggcgcagac cattgccgat
    19621 atggcgctgc gttatgtggt tatcacctcc gttgaccgtg atgacctgcg cgatggcggt
    19681 gcccagcact ttgcggattg cattactgcc attcgggaaa aaagcccgca aatcaaaatt
    19741 gaaactctgg tgccggattt ccgcggtcgt atggatcgtg ctctggatat tctgactgca
    19801 acgccaccag atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccgtcaggta
    19861 cggcctggtg cagattacaa ctggtcgctg aagctgctgg aacgctttaa agaagcacat
    19921 ccggaaatcc cgaccaagtc gggtctgatg gtgggactgg gtgaaaccaa tgaagaaatt
    19981 attgaggtaa tgcgcgacct gcgccgtcat ggtgtgacga tgttaacgct ggggcaatat
    20041 ttgcagccaa gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac
    20101 gaaatgaaag ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc
    20161 cgctcttctt accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat
    20221 aattacttac atctgtctga taaaaaaccc gctttttgaa gcgggttttt tgtatcgaac
    20281 aagatattga gggagcgtcc tgctcgccac gtcactcttt atgagagagc ttttcagcct
    20341 gaagatcaac gtctgcgcct tttttcgccg cagcatcgtc atcattcatc gccttcttga
    20401 accctttaat ggccgctcca aggtctccgc ccagcgtacg taacttctta gtcccaaaca
    20461 gcagaacgac cagcgccgca actaccagca gtttggtaat actaatctca cccatagata
    20521 ccttcttgac atataaccgg caacagaacg cgccttaaaa accagagaat attaacggtc
    20581 attcgacgcg cgcacacaat agcaatctgt aacaaggtga atcaagcgcc gagtaaaaaa
    20641 acatcataat aattgcggcg gcgcaaagcg acggttgttt aagacgggca gttgcgcgcg
    20701 cacctgacgc acacgttcgg gcgtcacttc cgccataatg agtgcaggca tttctgacgc
    20761 tgccgcaatg gtgacgccaa agggatctat aatccggctt tgcccgatat ttttgttccc
    20821 gcactccccc gccgccacca tataacaggt ggtatccagc gcacgagcgg caagcaacgt
    20881 tgaccagtga tgctctttga gcggcccgcg aacccaggcg gcaggaagtg ccaggatttc
    20941 agctccctgt aatgcctgtg ccagcgccag ctctggaaag cgtaagtcat aacaggtcat
    21001 cagaccgacc ttcatccctt ccacctccag taacggagcg atttcattac cagcatcaac
    21061 acggcgtgat tcctgaatgg caaatgcatc atagagatgc agtttggcat aacgggcgac
    21121 aatgtttcct gcctgtagcg ccaccagcat attccatgcg cgccccggcg ttgaaggaac
    21181 atgaatcgtc agaattgtcg tcatcatgtt acgtttactt tctcgccgta aacgtccgag
    21241 gaattcgcct tccagcagct gtgctgattt aaccgataga tctgcatcat gatcatcgcg
    21301 tgccagcaat gcttccggca ggacaaacag cgatacgtcg ttttccgccg cctgcgccat
    21361 caacgaggca caaatctcag cgttcttttc ccacacagat gtaacagcaa actgtcctgc
    21421 agcaaccagc attgccacct cctgtcagag tcgtaaaatt cgccaacgac gattattcgg
    21481 cgttacactt atcactcata caaatcaaat agcaggattt tgcagtgtta caacttcttt
    21541 tagcagtttt tattggcggt ggtacgggaa gcgtggcgag atggctgtta agtatgcgat
    21601 ttaacccgct gcatcaggcg attccgttgg ggacgctggc tgcaaatctg attggggcat
    21661 tcatcatagg aatgggattc gcctggttca gcaggatgac aaacattgat cccgtgtgga
    21721 aagtattaat caccaccgga ttttgtggcg gtcttacaac cttctcaaca ttttcggcag
    21781 aagtggtgtt tttgttacaa gagggccgct ttggctgggc gttactgaac gttttcgtca
    21841 accttctggg gtcttttgcc atgaccgcac tggctttctg gctgttttcg gcctcaaccg
    21901 cacactaaag accaaaaaaa acccgctgat taagcgggtt ttgaattctt gctgacgtat
    21961 cttacagagc gattacgttt gcagcagaag ggcctttggc accgttagtg atttcgaact
    22021 ctacgcgctg accttcagca agagttttaa aaccattagt ctggattgca gagaagtgta
    22081 cgaacacgtc tttgctgccg tcttccggag taatgaaacc gaatcctttg gactcattaa
    22141 accacttaac gttaccttta atcttagaca tcaaaattac ctttacatga aaaatagaca
    22201 caaatgctgt gtcggttacc agtacaccaa ttgtggtacg ctttgtccag tgcaacttta
    22261 ctaaaaagtg ataaaagtcg cgttattttt gatgttactt attttagtta ttgattttaa
    22321 agaagttact aaaacttcat ttgtctcaaa actgaaagcg catccaggca aagtacacat
    22381 tgccattgtt gtaggtaccc ggaatatagg tcatctgaaa agtcactgga ccataaccca
    22441 cggaggccaa tggcagtaga accgggagag ggatgtaatt ccagttatca cgtgccgtta
    22501 cgccagcggt gaatcccaga cctaaatgaa aattttcatc cgccagcggt cgccaggtac
    22561 tttcccatcc gtatccggca atcggttccc atttgttcca cgagtcctta aatgccatgg
    22621 catacaggcc atgccagttt cctttttcat cccaacgcga caggccaaaa ccgccacccc
    22681 acggtcgctc gttatagcga tcggtttttt ctttgtcgta agcgaaacgt gcatgccagg
    22741 tgatggcagg aatatataaa tcataatgtt caggctgttg ccaggtttgt gcaatatttt
    22801 ctctaaacgt tgtcatccac tcatctgcgt tagcaaaaac tttaccaacg ctgattaact
    22861 gaataaaaac aaaggaaaag atagcgacat atttactcac gttcatttgt gaccataaaa
    22921 catttatcaa aaatctacta ctagcatagc aaagctacaa ttaacataac cttaatagcc
    22981 ccaacataaa gaataatctg aatagctgct ctatttattt ccgcttgttc aagttaaata
    23041 tttccgcgca tcgatttaaa atgacacatc gttttctgcg atgggaatgg tcataaaaga
    23101 aaaa
//
LOCUS       DAPGRN010000072        22843 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPGRN010000072 DAPGRN010000000
VERSION     DAPGRN010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.072"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(630..1721)
                     /gene="ychF"
                     /locus_tag="RHW33_002994"
     CDS             complement(630..1721)
                     /gene="ychF"
                     /locus_tag="RHW33_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV1646475.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1838..2422)
                     /gene="pth"
                     /locus_tag="RHW33_002995"
     CDS             complement(1838..2422)
                     /gene="pth"
                     /locus_tag="RHW33_002995"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV1646476.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2700..2978
                     /gene="ychH"
                     /locus_tag="RHW33_002996"
     CDS             2700..2978
                     /gene="ychH"
                     /locus_tag="RHW33_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV1646477.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3033..4712)
                     /gene="dauA"
                     /locus_tag="RHW33_002997"
     CDS             complement(3033..4712)
                     /gene="dauA"
                     /locus_tag="RHW33_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV1646478.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4837..5784)
                     /gene="prs"
                     /locus_tag="RHW33_002998"
     CDS             complement(4837..5784)
                     /gene="prs"
                     /locus_tag="RHW33_002998"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV1646479.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5935..6786)
                     /gene="ispE"
                     /locus_tag="RHW33_002999"
     CDS             complement(5935..6786)
                     /gene="ispE"
                     /locus_tag="RHW33_002999"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV1646480.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGANLS
                     PLHRAML"
     gene            complement(6786..7409)
                     /gene="lolB"
                     /locus_tag="RHW33_003000"
     CDS             complement(6786..7409)
                     /gene="lolB"
                     /locus_tag="RHW33_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV1646481.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7623..8879
                     /gene="hemA"
                     /locus_tag="RHW33_003001"
     CDS             7623..8879
                     /gene="hemA"
                     /locus_tag="RHW33_003001"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV1646482.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8921..10003
                     /gene="prfA"
                     /locus_tag="RHW33_003002"
     CDS             8921..10003
                     /gene="prfA"
                     /locus_tag="RHW33_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV1646483.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10003..10836
                     /gene="prmC"
                     /locus_tag="RHW33_003003"
     CDS             10003..10836
                     /gene="prmC"
                     /locus_tag="RHW33_003003"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV1646484.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10833..11225
                     /locus_tag="RHW33_003004"
     CDS             10833..11225
                     /locus_tag="RHW33_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV1646485.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11229..12038
                     /locus_tag="RHW33_003005"
     CDS             11229..12038
                     /locus_tag="RHW33_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV1646486.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVIL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12074..12928
                     /gene="kdsA"
                     /locus_tag="RHW33_003006"
     CDS             12074..12928
                     /gene="kdsA"
                     /locus_tag="RHW33_003006"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV1646487.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13076..13183)
                     /locus_tag="RHW33_003007"
     CDS             complement(13076..13183)
                     /locus_tag="RHW33_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV1646488.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13612..13719)
                     /locus_tag="RHW33_003008"
     CDS             complement(13612..13719)
                     /locus_tag="RHW33_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV1646489.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAEIVGWWRNRK"
     gene            complement(14124..15224)
                     /gene="chaA"
                     /locus_tag="RHW33_003009"
     CDS             complement(14124..15224)
                     /gene="chaA"
                     /locus_tag="RHW33_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV1646490.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15494..15724
                     /gene="chaB"
                     /locus_tag="RHW33_003010"
     CDS             15494..15724
                     /gene="chaB"
                     /locus_tag="RHW33_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV1646491.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15882..16577
                     /gene="chaC"
                     /locus_tag="RHW33_003011"
     CDS             15882..16577
                     /gene="chaC"
                     /locus_tag="RHW33_003011"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV1646492.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(16621..16974)
                     /gene="ychN"
                     /locus_tag="RHW33_003012"
     CDS             complement(16621..16974)
                     /gene="ychN"
                     /locus_tag="RHW33_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV1646493.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            17160..18554
                     /gene="ychO"
                     /locus_tag="RHW33_003013"
     CDS             17160..18554
                     /gene="ychO"
                     /locus_tag="RHW33_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV1646494.1"
                     /translation="MSRSVPRIIPFYLLLLAAGGTANAHSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVVLSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18555..19205)
                     /gene="narL"
                     /locus_tag="RHW33_003014"
     CDS             complement(18555..19205)
                     /gene="narL"
                     /locus_tag="RHW33_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV1646495.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(19198..20994)
                     /gene="narX"
                     /locus_tag="RHW33_003015"
     CDS             complement(19198..20994)
                     /gene="narX"
                     /locus_tag="RHW33_003015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV1646496.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            21333..22724
                     /gene="narK"
                     /locus_tag="RHW33_003016"
     CDS             21333..22724
                     /gene="narK"
                     /locus_tag="RHW33_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV1646497.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5619 a         5686 c         5882 g         5656 t
ORIGIN      
        1 tccctaagtt gtcgttactt attccaattc gttactatga atatggcaaa aatattacaa
       61 caaacagcgg ttaaaaaatt tggtgaagat tttttgtaat aatcctaatt agttacggga
      121 ttttcgcttg atgtatctgc ataacaaaaa caatatgcgc catttttgca ttactaataa
      181 gaaaaagcaa aaaacatcga tattcatttt tggtcaatgg gttttctggc ctatcgttgt
      241 tattgtgcta ctggttatca ttttcatctt gccaggatcg cacgtaatca cgttattcgg
      301 ggactttatc tgcgggatat tacccttcag gtaagtcctt ttctcgccag catattggag
      361 cgtgatcaat attgatcagc tgtgaagggg gctgatgtgt agtggaacaa aaactgacgt
      421 tatatcactg ctaatgagat atccgtcatc tgaacactta tgtctttaat tattctcgtg
      481 gttcactgta ggcaataagc acaaaagtgt aggatgttac aagaatgatt cggactcggt
      541 gaaatgaaaa atccacgcaa ttgcgtggat tttatatact tttgcgctct tcatgagatt
      601 tagcgaaacc tcatgagaca ataaattaat tagacgttga aaaggaagtt catcacatcg
      661 ccatctttca cgatgtaatc tttaccttct gcacgcattt tgcctgcttc tttcgcgcct
      721 tgttcacctt tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg gataaagcct
      781 ttttcaaaat cagtatggat tttgcccgct gcctgcggcg cggttgctcc aaccggaatg
      841 gtccatgcac gcacttcttt caccccagcg gtgaagtaag tttgcaggtt cagcagctta
      901 taaccggcac ggatcacacg gttcaggccc ggctcttcca gcccaagctc ctgcataaac
      961 tcgtcacgtt cttcgtcgtc cagttcggca atgtctgctt caacagcagc acaaaccgga
     1021 accacaacag aaccttcttt cgccgcgatt tcacgcacct ggtcaagata tgggttgttt
     1081 tcaaaaccgt cttcgttgac gttggcgatg tacattgttg gtttcagcgt caggaagctc
     1141 aggtaacgaa tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag catacctgcg
     1201 ttttccagct ggggcaggca tttttccagg accgccagct cagctttcgc gtctttatcg
     1261 ccacctttgg ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc gaggtctgcc
     1321 agcgccagtt cggtgttgat aacttcaata tcgtcagccg ggttaacttt gccggaaacg
     1381 tgaatgatgt tgtcattttc aaagcagcga acaacgtgac cgatcgcttc ggtttcacgg
     1441 atgttggtca ggaactggtt acccagacct tcgcctttcg atgcgccttt taccagaccg
     1501 gcgatatcga caaattccat ggtcgtggga agcgtacgtt gcggttttac gatttcagcc
     1561 agttgatcca ggcgagggtc aggcattggt acgacgcctg tgttcggctc aatggtgcag
     1621 aatggaaagt tggccgcttc aataccggct ttggtcagcg cgttgaacag ggtagatttc
     1681 ccgacgttgg gcaaaccgac gataccgcat ttgaatccca tgatttaaat caccttaata
     1741 tcttaataat caacctgtta ttgctaacag attgcagaaa tggaaataac tttgcctatt
     1801 atacacggca ctcggcaaaa atgccgcaga caacgactta ttgcgcttta aaggcgtgca
     1861 atcggttcgt tgctttggtc aagccatctg taaaccacat ttcagtacaa cgcgccgctt
     1921 cgtcgatggc ttcatcaatt aacttctgtt cactaacagg cggtttgcct aacacaaaac
     1981 cgacaacttt atttttatcg cccggatgac cgattccgat gcgtaaacgg tgaaagttag
     2041 ggttattacc caatttactg atgatgtctt tcagtccatt gtgaccacca tggccaccgc
     2101 ccaacttaaa tttggcgacg ccaggaggca gatccagttc gtcgtgggcc accagaattt
     2161 cgtccggatt aatgcggaaa aaactggcca tcgccgcaac ggctttgccg ctgagattca
     2221 taaatgtagt cgggactaac aggcggacat cttcgcctcc aagggtgact cgcgaagtat
     2281 aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc agtaagtcaa
     2341 cgaaccaggc accagcatta tgtcgcgttg cggcgtattc agcaccgggg ttcgccaggc
     2401 cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg tgtacataac tggcgcgtag
     2461 tttactggtt gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa
     2521 ttgcctgagt ggactattag aaagtcaggg tgttcaggcg tttatttgta aagttttgtt
     2581 gaaataaggg ttgtaattgt gatcacgccc gcacataacc cacagggtgt tgtctatact
     2641 ttacacataa ggaagagggg tatcccctgt tacaacccag aaagttccgg aggtgacata
     2701 tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat ggggttgggt ctggtggtaa
     2761 tggtggtcgg cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt
     2821 atttcgcaca tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg
     2881 cccgtgttgg cgggcatgaa caggtgtgcg accgttactg gtgggttcgc cactatgaca
     2941 aacgttgccg ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc
     3001 agtcagtttg cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga
     3061 ttcgggaaga acgcgaggcg tcccgggatc ggttgaatgc ctgcgcgagc catagtgcgc
     3121 agtggctgga attccacgtt gcacacgcgc agttcacatc cttcgggcag acgcttcaca
     3181 aaacgctgga acgcatcaag accaccagca tcaagtaccg gaacggcatc ccacttcaga
     3241 atcacaatcc gtttgccttc aagacgtgac tccaggtccg tgaacaagcc ttcagcagca
     3301 gcaaaaaaca gcgggccaat aacgcgcaga accaggacat cgtctggaac atctacgact
     3361 accggtgcca ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg
     3421 atccccacgc tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc
     3481 atgacgatga tgtcatcttt cggcgcatga cgcagcaagt cgaccacttt atgcgcttca
     3541 ctcatgttcc acgccaccat caacagcagg gctgccatag cggaaagcgg cagccaggag
     3601 agcagcggtg ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgccgag
     3661 ataggggaag ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg
     3721 gtaataccac caaagaacgg agcgatgata ttccccagtc cctgtccaac cagttcgcta
     3781 ttcgccttgt gtttcgtccc ggtcatacca tccagcacca cggcgcagag cagagattcg
     3841 attgcgccga gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag
     3901 gttagcgtga attctgaatt aggcagatcc cacggtagca ccagttgcgg cagcagttgc
     3961 ggaataccgt taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta
     4021 gcaacatgtc cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc
     4081 ggaaggtgac cgggtaaacg aatgcccaga cgcggccaga aaacaagaat acctagcgtc
     4141 acaatgccaa tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg
     4201 actttttgta gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc
     4261 tgcatggtac cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga
     4321 atatactcaa tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa gatccccgac
     4381 agcaaggtcg caaccagcag tcctgccagt ccaaactgtt gcgacacggg atagagaatt
     4441 accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg
     4501 acaatccccg caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata
     4561 gccaacgcca tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca
     4621 cgggtaaacc gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa
     4681 ggcatcacat gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat
     4741 ctgtcaacta aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa
     4801 aaaacccgcc gcagcgggtc tttgagccgg gttcgattag tgttcgaaca tggcagagat
     4861 cgattcttcg ttgctgatac gacgaatcgc ttcggccagc atacctgaca gggtcagagt
     4921 acgcacgttc ggcagtgatt tgatttcatc gctcagcgga atggtatcgc agacaacgac
     4981 ttcatcaatt acagagttac gcaggttgtt cgccgcgttg ccagagaaga tcgggtgagt
     5041 cgcgtacgca aatacacgtt tagcaccacg ttctttcaga gcttcagcag ctttacacag
     5101 cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag tcacgacctg caacgtcacc
     5161 gatgatatgc atcacctggg aaacgttcgc acgcggacga cgtttgtcga tgattgccat
     5221 atcggtatcg ttcagcagct tagcgatagc gcgggcacgc acaacgccgc cgatgtccgg
     5281 agaaaccaca attgggttat ccagattcag ctgcagcatg tcttccagca ggatcgggct
     5341 accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag cgtgcagatc
     5401 cactgtcagc acacggtcaa caccgacgct ggagaggaag tctgcaacca ctttcgcagt
     5461 gattggtaca cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa agtaggggat
     5521 aacagcggtg atacgacctg cggaagcacg acgcagggca tcaaccataa cgactaattc
     5581 catcaggttg tcgttagtag gggcacaagt ggactggatg atgaaaatat caccaccgcg
     5641 tacattttca ttaatttgta cgctgacttc gccatcgcta aagcgaccta cagcggcgtc
     5701 gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct agttccgggg tggcgttacc
     5761 agcaaaaagc ttcatatcag gcacgagaag aacctcaggc atgcgtccat tggtggaaag
     5821 aatctgccga aaactgtgcg ggccaggcat gatcctttcc aggcggtgta ttaaagagcg
     5881 cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc cggcttaaag
     5941 catggctctg tgcaatgggg aaagattagc gcctttcgcc acaaagccat tgagccattc
     6001 cggggcttgc tctagcacct ggcgggcttc agactctgta tcaaattcag caaagacaca
     6061 ggcccctgtc ccagtcaggc gcgacggggc gtattctaac agccaggaaa gcaccgcatc
     6121 aacctcgcga aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt cacattttag
     6181 caacgtttct attgaccttt ttggcgtatt gcgcgggagt tcaggatctt taaaaatcac
     6241 cggagtcgga atacttacac cagggtgcgc caccagatac cacttctctg gcggatccac
     6301 cggggttagt atttcaccaa cgccttcggc aaacgcggca tgcccccgaa caaagacagg
     6361 aacatctgcg cccagcgtca gccccatttc cgccagctca tccatgctta gcccgcattg
     6421 ccagagatga tttaatgcca ccaggaccgt cgcggcattg gatgaaccac cgccgagacc
     6481 gccgcccatc ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg tcggaagacg
     6541 cccgctgtct gccgcagttt tcatcaacaa ccgcgctgcg cgaacgatca ggttatcttc
     6601 atgttccacg ccttcaacgg gcgttaacag acggatatcc ccatcgtcac gaagctcaat
     6661 gctgatggtg tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt ggtaaccatc
     6721 cgcacgctga ccggtaatgt ataaaaacag attaagtttt gccggagagg gccactgtgt
     6781 ccgcattatt tcactatcca gttatccatt tttaacttga tgcgttgacc accgtcggtg
     6841 agttccatat tggctggcat cgcaggttgc gttttggtgt cataaccacc ataaacaacc
     6901 ttccagtttt tgccattctg gctgtaggta atttcgctca ggcggtactg gtcgtccagt
     6961 ttgtagtcgg ttgcatcacc cggtaaacct aaaatccact ggcgcaagct gttgagcgga
     7021 attggcattc cagtcaattt gccaatcatc tcttcggcgt catcggcggt ataacgctga
     7081 cctttattgt cgactaactg cacgttaccc ggttgagcat tcagctccag ttccgtgctg
     7141 cccaatgggt tagtgaggag cagacggtag cgatcctggc cggtttgctg ccagaaaaag
     7201 cgggcgtaca ctttttgttg gtcagaaata taagcgaacg cgccgcgagt ttgatactga
     7261 ttaagattgc gcacgtcttg ctgatgctga cgccattgtg gcgagtccgg gcttttgcca
     7321 ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa gagcagccag cggtagcagg
     7381 cggataagac gaaaatcggg caggggcata gtgatgacaa gtccttgaga tacgttgcag
     7441 ttataaccct taatgctagc gttaccgtcc gctatcgtct atgttcaagt tgtcttaatt
     7501 gccagaatct aacggctttc ggcaattact ccaaaagggg gcgctctctt ttattgatct
     7561 tacgcatcct gtatgatgca agcagactaa ccctatcaac gttggtatta tttcccgcag
     7621 acatgaccct tttagcactc ggtatcaacc ataaaacggc acctgtatcg ctgcgagaac
     7681 gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga cagcctgctt gcgcagccga
     7741 tggtgcaggg cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat cttagcgttg
     7801 aagagcagga taacctgcaa gaggcgttaa tccgctggct ttgcgattat cacaatctta
     7861 atgaagaaga tctgcgtaaa agcctctact ggcatcagga taacgacgcg gttagccatt
     7921 taatgcgtgt tgccagcggc ctggattcac tggttcttgg ggagccgcag atcctcggtc
     7981 aggttaaaaa agcgtttgcc gattcgcaaa aaggccatat gaaggccagc gaactggaac
     8041 gcatgttcca gaaatctttc tctgtagcga aacgcgttcg cactgaaaca gatatcggtg
     8101 ccagcgctgt gtctgtcgct tttgcggctt gtacgctggc gcggcagatc tttgaatcgc
     8161 tctctacggt cacagtgttg ctggtaggcg cgggcgaaac catcgagctg gtagcgcgtc
     8221 atctgcgcga acataaagta cagaagatga ttatcgccaa ccgcactcgc gaacgtgccc
     8281 aaattctggc agatgaagtc ggcgcggaag tgattgccct gagtgatatc gacgaacgtc
     8341 tgcgcgaagc cgatatcatc atcagttcca ccgccagccc gttaccgatt atcgggaaag
     8401 gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg gtggatattg
     8461 ccgttccgcg cgatgttgag ccggaagttg gcaaactggc gaatgcttat ctttatagcg
     8521 ttgatgatct gcaaagcatc atttcacaca acctggcgca acgtaaagcc gcagcggttg
     8581 aggcggaaac tattgtcgct caggaaacca gcgaatttat ggcgtggctg cgagcacaaa
     8641 gcgccagcga aaccattcgc gagtatcgca gccaggcaga gcacgttcgc gatgagttaa
     8701 ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc gcaagccatt atgcaggatc
     8761 tggcatggaa actgactaac cgcttgatcc atgcgccaac gaaatcactt caacaggccg
     8821 cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga cagcctcggg ctggagtagc
     8881 agtacatcat tttctttttt tacagggtgc atttacgcct atgaagcctt ctatcgttgc
     8941 caaactggaa gccctgcatg aacgccatga agaagttcag gcgttgctgg gtgacgcgca
     9001 aactatcgcc gaccaggaac gttttcgcgc attatcacgc gaatatgcgc agttaagtga
     9061 tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa gatatcgaaa ccgcacagat
     9121 gatgctcgat gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg aagctaaaga
     9181 aaaaagcgag caactggaac agcaattaca ggttctgtta ctgccaaaag atcctgatga
     9241 cgaacgtaac gccttcctcg aagtccgagc cggaaccggc ggcgacgaag cggcgctgtt
     9301 cgcgggcgat ctgttccgta tgtacagccg ttatgccgaa gcccgccgct ggcgggtaga
     9361 aatcatgagc gccagcgagg gtgaacatgg tggttataaa gagatcatcg ccaaaattag
     9421 cggtgatggt gtatatggtc gtttgaaatt cgaatctggc ggtcatcgcg tgcagcgtgt
     9481 tcctgctacg gaatcgcagg gtcgtattca tacttctgct tgtaccgttg cggtaatgcc
     9541 agaactgcct gacgcagaac tgccggacat caacccagca gatttacgca ttgatacttt
     9601 ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc gattcggcaa ttcgtattac
     9661 tcacttgccg accgggattg ttgttgaatg tcaggacgaa cgttcacaac ataaaaacaa
     9721 agctaaagca ctttctgtac tcggtgctcg catccacgct gctgaaatgg caaaacggca
     9781 acaggccgaa gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca gcgaccgtaa
     9841 ccgtacttac aatttcccgc aggggcgcgt taccgatcac cgcatcaacc tgacgctcta
     9901 ccgcctggat gaagtgatgg aaggtaagct ggatatgctg attgaaccga ttatccagga
     9961 acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa taatggaata tcaacactgg
    10021 ttacgtgaag caataagcca acttcaggcg agcgaaagcc cgcggcgtga tgctgaaatc
    10081 ctgctggagc atgttaccgg cagagggcgt acttttattc tcgcctttgg tgaaacgcag
    10141 ctgactgacg aacaatgtca gcaacttgat gcgctactga cacgtcgtcg cgatggtgag
    10201 cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt tgtttcgcca
    10261 gcgaccttaa ttccgcgccc ggatacggag tgtctggttg agcaggcact ggcgcggttg
    10321 cctgaacaac cttgccgtat tctcgatctc gggacgggta ccggggcgat tgcgcttgcg
    10381 ctggctagcg agcgcccgga ctgcgaaatt accgctgtag atcgtatgcc cgatgctgtc
    10441 tccctggcac aacgtaatgc tcagcatctg gcgatcaaaa atatccacat tctgcaaagc
    10501 gactggttta gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa tccgccgtat
    10561 attgacgagc aggaccctca tcttcaacaa ggcgatgtcc gctttgagcc gctcactgcg
    10621 ctggttgcgg cagacagtgg aatggcagac atcgtgcata tcatcgaaca gtcgcgtaac
    10681 gcgctggtat ccggcggctt tctgcttctg gaacatggct ggcagcaggg cgaagcggtg
    10741 cgacaagcat ttatccttgc ggggtatcat gacgtcgaaa cctgccgtga ctatggtgat
    10801 aatgagcgcg taacgctcgg ccgctattat caatgacaag tttttctaca ctgcttagtg
    10861 ttcatcttat tagtatcgcg ctttctgttg ggctattaac cttacgtttc tggctacgtt
    10921 atcagaagca tccacaggca tttgctcgct ggacgcgcat tgtgccgccg gttgtcgata
    10981 cggtgttatt gctaagcggc attgcgttga tggctaaagc gcacatcctg ccattttccg
    11041 ggcaggcaca gtggctgact gaaaagctgt ttggagttat catttatatc gttttgggtt
    11101 ttattgcact cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt gccttcccgc
    11161 tggcgttggt ggtgctgtac atcatcatta aactcgccac cacaaaagta ccgttactgg
    11221 ggtaagtcat gagatcgtta gctgatttcg aatttaataa agcgccattg tgcgaaggca
    11281 tgatcctggc ttgcgaagca atccgccgcg attttccctc gcaagatgtt tacgacgaac
    11341 tggagcgtct cgttattctg gcgaaggaag aaatcagcca gcttctgcct ttagaagagc
    11401 agctggaaaa actgatcgcg ctgttttacg gcgactgggg atttaaagcc tcacgcggcg
    11461 tttatcgtct ttccgatgca ttatggctgg accaggtgtt aaagaatcga cagggcagtg
    11521 cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg ccgctgctgc
    11581 cagtgatttt ccctacgcag ctgatattgc gcattgaatg tccggatggc gaaatttggc
    11641 tgattaatcc ttttaacggt gaatcgttaa gcgaacatat gctggacgta tggttaaagg
    11701 gaaatatcag cccgtcggcg gaactgtttt atgaagacct tgatgaagct gataacattg
    11761 aggtaatccg caaattactg gacacactca aagcctcgtt gatggaagaa aatcagatgg
    11821 agctggcgtt acgcaccagc gaagctttat tacaattcaa ccctgaagat ccctatgaaa
    11881 ttcgcgatcg cgggttgatt tatgcgcaac tggattgcga acacgttgcg ttgaacgatt
    11941 taagttattt cgttgaacaa tgtccggaag acccgatcag cgaaatgatc cgtgcgcaaa
    12001 taaataacat cgcgcataaa catattgtgc tgcattaatt aatcgacatt ttactcaaga
    12061 ttaaggcgat cctatgaaac aaaaagtggt tagcattggc gacatcaacg tagcaaatga
    12121 cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc tggcgatgcg
    12181 catttgcgag cactacgtaa ccgtgaccca gaaactgggt atcccttacg tgttcaaagc
    12241 ctcttttgac aaagccaacc gctcctccat ccactcttat cgtggaccgg gcctggaaga
    12301 agggatgaaa atcttccagg agttgaagca gacttttggc gtgaaaatta tcaccgacgt
    12361 tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc agttgccggc
    12421 gtttcttgct cgccagactg acctggttga agccatggcg aaaaccggtg cggtaattaa
    12481 cgtcaagaaa ccacagtttg tcagcccggg acagatgggt aatatcgttg ataaattcaa
    12541 agaaggcggc aacgaaaaag tgattctttg cgatcgcggt gctaacttcg gctatgacaa
    12601 cctggttgtc gatatgctgg gcttcagcat tatgaagaaa gtgtctggta actcgccggt
    12661 gattttcgac gtgacccacg cactgcaatg ccgcgatcct tttggcgcag cttccggtgg
    12721 tcgtcgtgct caggtggctg agctggcacg agccggtatg gcggtaggtc tggcggggct
    12781 gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt gatggtccat ccgcgctgcc
    12841 gctggctaaa ctggaaccgt tcctcaagca gatgaaagcg attgatgatc tggtgaaagg
    12901 tttcgaagaa ctggatacta gcaagtaatc ttttttgctt gaaaaataaa gtcttagcgt
    12961 tctgcgttaa gacttttttc atgggtgccg gatacaaaaa aggccgcagg ctgttaccct
    13021 gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt tcgccttact
    13081 tccggttacg ccaccagccg acaatcgctg cggtaataat tcccgccagg atcggtgctg
    13141 ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg ccttgttgta
    13201 atgacaacat tttgcggcta ttcttgaatt gttgatggtt caagattagc ccccgttctg
    13261 ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
    13321 gggataaagc ccccgcaaca ttgcgcctca ccggataacg ccggcttggt gtggatacta
    13381 cgactcaatt catcttcact tcatccctga aatgtttgca atgaagagtg cattccggtt
    13441 tttcaacagc tgttacagtc gtttcatgag tgctctggat gatgcttcca gctcgggttg
    13501 ccaatattta cttgtggaag tgataaagac aaaaatggcc gcaggctgtt acccctgcgg
    13561 ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac cttacttccg
    13621 gttacgccac cagccgacaa tctctgcggt aataattccc gccaggatcg gtgccgccag
    13681 gtcgtgccag aaagtcatgg caaactgcgc gagcgtcata tagccgcctt gttgtaatga
    13741 caacattttg cggctattct tgaagtgtct ggtttcaaga ttagcccccg ttttgttgtc
    13801 aggttttacc tctcaacgtg cgggggtttt ctctttccag caaccaatgc caccagggat
    13861 aaagcccccg caacattgcg cctcaccgga taacgccggc ttggtgtgga tactacgtct
    13921 caattcatct tcacttcatc cctgaaatgt ttgcaataaa gagtacattc cggcttttca
    13981 acagctgttg cagtggtttc gtgcaggttg tggtacaggc tcgcaattct gattaacgac
    14041 ctgtccaggt agtatgaaga aatacaataa gcaggaattt atattatctc ccccgatttc
    14101 cgtcatcgga ggagatataa ctgtcaggca aatatcgtca tcaaataggc ggcaaacagt
    14161 gccagatgcg ctgcgccatt gagcacgtta gtacgtccgg tggagaaaga gatatggcac
    14221 agcactaaag aggccaccat caccaccatt tctggcgcac caagtgcaaa ctgcaattcg
    14281 ttacccgtca taaatgcaat tagcgtgacg acaggtacgg taagcgaaat tgttgctaac
    14341 actgaaccaa agaacagatt catcgcgcgc tggacctggt tgttcaacac tgcttttaat
    14401 gcacctaaac cttccggcga cagaatcaac agtgccacca ggaagccagt aaaggcgaca
    14461 ggggcattca tgctgtcgag caaagtctcc agcgggctgg cgttcatttt ggtgactgca
    14521 ataacggcaa tcaggtggat aatcaaccag atagcatgcc acaggctgct atgggcagac
    14581 ggtttaccgt ggtgcgggtc gtcgtcatca ctgtcatctt cgtgctcgta gacaaacaaa
    14641 ctttgatgcg ttttggtctg gatcagcaaa aatacgccat acattgccgc agaaattaat
    14701 gctaccagta acgcctgacc ggttgaaaaa ttcgccgcag gcagagccat tggaaatacc
    14761 agtacgatta tcgccagggg gaacagcgca attaaatact gcttaatacc aaacagattc
    14821 atatattggg tggcaaactt acgaccgccc aacaataatg aaaagccgac cagcccaccg
    14881 gtaacaatca taatgatcga atagagcgta tcacgcatta gcgttggcgc ggcgtcgccg
    14941 gttgccatta aagctgaaat caaactaact tcaagaataa ccactgaaag gctaagaata
    15001 agcgaaccgt aaggttctcc caggcgatgg gctaatacgt ccgcatgacg gacaacacta
    15061 aaggcgctac ttaaaatacc aataagcgca agaagattga tggcaatgac cactggtagt
    15121 gtctggctgc ttccccatag gaacagcact accagcgcca gaaccgggaa aataagcgaa
    15181 gtctccttgt ggcgggtttt taccgcctct tgagcatttg acattatggt tatccctttg
    15241 cagatgaatt tatcgaaaat gtaaaaaata ggtaggaaaa ataacagaaa gtgtctggat
    15301 atcggtaaca ttttacgaat ttttaccccg ttgtcgattt tttactcatt ggggcataaa
    15361 aataagtaat acgtttagac aatgtttgtt ttagcgtttc ttaagaacag tctgacatga
    15421 aaattcttat gttttggcaa gagtagatat tgttgaccac acttaatgtt caactttgta
    15481 aaaggagtca acgatgccgt ataaaacgaa aagcgacctt ccggaaagcg taaagcacgt
    15541 tctaccgtct catgcccagg atatctataa agaagcgttc aacagcgcat gggatcaata
    15601 taaagataaa gaagatcggc gtgatgacgc cagtcgcgaa gaaaccgcgc ataaagtggc
    15661 ctgggctgct gtgaagcatg aatatgccaa aggggatgat gataaatggc ataaaaaatc
    15721 gtaaaaccgg tagcttagtt aaagctattc gtgcggtgtt gccttgcaag tggtccgtgg
    15781 attgcatatt gtcccgttag tggtttcaaa atgagcagta aaaatgtccg gaagacacca
    15841 aaaagttgtc gcagggaagt atgcagtggc ggaggtgtaa ggtgataacg cgtgatttct
    15901 tgatgaatgc cgattgtaaa acggcatttg gtgccattga agaatcactc ttatggtcag
    15961 cagaacaacg ggcggcttcg ctggcggcga cgctggcttg tcgacctgat gagggaccgg
    16021 tgtggatctt cggttatgga tcgttgatgt ggaatccggc actggagttt accgagtcgt
    16081 gcaccggtac actggttgga tggcatcgcg cgttttgcct gcgcctgacc gccgggcgcg
    16141 ggactgcgca ccagccggga cggatgcttg cactaaaaga gggcggacgt accacaggcg
    16201 tcgcctatcg actgccagaa gagacgctgg agcaggaact aaccctgttg tggaagcgcg
    16261 agatgattac cggctgttat ctgccaacct ggtgtcagct tgatcttgat gatggacgca
    16321 cagtaaacgc cattgtgttt attatggacc cgcgacatcc agaatatgaa tctgacactc
    16381 gcgctcaggt catcgcgccg ttgattgcgg cggcgagcgg tccgctggga accaacgcac
    16441 aatacctgtt ttcactggaa caggagctta tcaaactggg aatgcaggac gatgggctga
    16501 atgatttgct ggtatcggta aaaaaactgc tggcggagaa ttatccggat ggtgtgttgc
    16561 ggccgggatt cgcctgagta aacttccctc atcgtggggc gtcagacgcc ccctcaaaca
    16621 ttaaaatgtg agcactttat cggctgacag cgtccattgc gccagttcca caagagtacc
    16681 gatttccacc ccatcaatca gaggaagtgt actaatcccg cgcccgtcgg tacaggtttt
    16741 gcacaatttc accggtacat tctgagcggt aaggatctcc agcatttgct gaatgttgta
    16801 gccttcccct ggtttttgcc cgcgcaaccc ggctgtgacc gcatcagaca tcaggaacag
    16861 acgcagatcc agattgctct cctgctctcg taacgcaatg gccagccgca agctgttaaa
    16921 caaggattcg ctcccgtaag gtgcgccatt ggcaacgatc acgatttttt gcattattta
    16981 ctcctgtatt cagggaatta gacactcatc ttctatctta ctgcttctgc agcgtctgac
    17041 caatcggtca catttttaag gattttcctg aaagcgcgag aaaatacgac aaaagttgcc
    17101 agtaatcgtt attctttaag gctatggttt ttcatttttt accggaagtt accgacgttt
    17161 tgagccgttc cgttcctcgc attattccgt tttatttact cttgctggcg gcaggcggta
    17221 cagctaacgc acactctacc ttcgagcaaa aagcggcaaa tccctttgat aataacaatg
    17281 atggtctgcc ggatttaggc atggcacctg aaaatcatga tggggaaaaa cactttgctg
    17341 aaattgtgaa agatttcggc gaaaccagta tgaatgataa cgggctggat actggcgagc
    17401 aggcaaaagc tttcgcattg ggaaaagtcc gcgacgcgct tagtcaacag gttaatcagc
    17461 acgtagagtc gtggctatca ccgtggggaa atgccagtgt tgacgttaaa gtggataacg
    17521 aaggtcattt taccggcagt cgtggaagct ggtttgtgcc gttacaagat aatgatcgtt
    17581 atctcacctg gagccagctt ggtcttactc agcaggatga tgggctggtg agcaatgtgg
    17641 gcgttgggca acgctgggcg cgcggcaact ggctggtggg ttataacact ttttatgaca
    17701 acttgctgga tgaaaatctt cagcgagcgg gctttggtgc cgaagcgtgg ggcgaatatt
    17761 tgcgactatc ggcaaacttt tatcagccgt ttgctgcatg gcatgaacag acagccacgc
    17821 aggaacaacg gatggcgcgc gggtacgacc tgacagcccg gatgcgcatg ccgttctatc
    17881 aacacctcaa taccagtgtc agcgtagaac agtattttgg tgatcgtgtt gatttgttta
    17941 actctggtac gggttatcac aatcccgtcg tgttgagtct gggattaaat tacacccctg
    18001 tgccattagt cactgtgacg gcccagcata aacagggtga aagtggcgag aatcaaaata
    18061 acctcgggct gaatcttaat taccgctttg gtgtaccgct caaaaaacaa ctttctgcgg
    18121 gcgaggttgc cgaaagtcag tcgttacgtg gtagtcgcta tgataatccg cagcgaaata
    18181 atctaccgac tcttgagtac cgacagcgaa aaacgttaac ggtgtttctg gcgacaccgc
    18241 cgtgggatct aaaacctggc gaaacagtgc cgctgaaatt acaaatccgc agtcgttacg
    18301 gtattcggca actgatttgg cagggcgata cgcagatatt aagtttgacg ccgggcgcac
    18361 aagccaacag cgcggagggc tggacgctga tcatgcctga ctggcagaac ggggaagggg
    18421 cgagcaatca ctggcgattg tcggtggtgg tggaagataa ccaggggcag cgtgtctcct
    18481 ccaatgagat cacgctaacg cttgtcgaac cgttcgacgc attgtcaaac gacgaactgc
    18541 gctgggaacc gtaatcagaa aatgcgctcc tgatgcaccc ataccgctgc ttccacgcga
    18601 gacttgagct tcattttctt cagcatgtgc ttgacgtgca cttttactgt gctttcggtg
    18661 atgtccaggc ggcgggcaat catcttgttc ggcaaaccct gggcaatcag cttgagaata
    18721 tcgcgctcgc gtggggttaa ctggttaaca tcgcgctcag tcgtggcacg gttagcgcgc
    18781 aagctggcgg ccagaacagg cgttaatgct tcgcttaata ccatttcgcc agcagctgcc
    18841 tgatgcaatg ctttcagcag atcttccggt tccatatctt ttaacagata gccatccgcg
    18901 ccgcgtttca gtgcggtgac cacatcttct tcatggttag agacactgaa taccacaatg
    18961 cgccctgaga gggacttttc gcgcagttta tccagcgttt ccagaccgtt catgccgggc
    19021 atattgagat ctaacaggat cagatcggga tcaagagatt ccgccagttc aataccctgt
    19081 tcgccattac tcgcttcgcc aaccacggtg atatctggtg ccatactgat aagctgtttt
    19141 acgccagttc gcagcatcgg gtgatcgtca atcagcagga tagtagccgg ttcctgatta
    19201 ctcatgggta tctccttgga cgtctgtgaa agttttttcg ggaataaagg tgaccaccac
    19261 ttcggtgcca cctgattcac gacggcggac gcggcaatcg cctcgtaaac tttgcgcacg
    19321 gtcgcgcatt attatcatgc cgtagtgatt gctgcggatg gcattttcag gcacgccgca
    19381 gccgttatcc tggacggtca gtttgacctg attatcgttt tgcgccaccg tcaccacgac
    19441 ctcactcgct tgcgaatgtt tgagggcgtt acttaatgcc tcacgggcaa tttgcaacaa
    19501 gtggatagcc tgatgcgaag gcaccagacg aggcggcaat tgataatcca gcttcaccgg
    19561 gaagccaaat ttggcgctgt actcttcgca actcgcctcc agcgccggac gtaatccagg
    19621 ctcggtgagc tgcaagcgga atgtggtgag caattcacgc aactgcgccc aggatgcatt
    19681 cagttcgtta cggatctgac ttaacagttc gcggctgctt tctggcagcg catcgccctg
    19741 catctgtaaa caactcacct gcatcttcat gcaagagaga gattgggcaa tagaatcatg
    19801 cagttcgcgc gcaatggtgg cacgctcttc catcacgatc aactgttgct gacgttcctg
    19861 atgccgatcc agcgccagcg tggcggtgag ttgttcaacc agggtatcca ccagttgttg
    19921 ttgatcatgg ctaagatgac gcccctgcgg cagggtcgcc agcaaaatac cgtactgcgt
    19981 atgagagtca gccagccgcc acttcagggt cgtgccgcga tcaccaacgg gtaatacgcc
    20041 gcgcgggcag agctggcagc ctttatcatc acaagtcata tctggctggc aggtaaactc
    20101 ctgatgattc tcttcatcat cagtgtcata cacccgcagt tcgatatcac gtagcagggt
    20161 taaattctgt aagccgttga gtacaggtga caggcgttca cacagcgggg cgcgggaatg
    20221 caaacggcgg ttagcctgcc ataaaaaaga gaggatctga tttttatgct ccagcccggc
    20281 ggttttctcc tgaacccgct gctcaagtac ggcataactt tcggccagtt ctgcagacat
    20341 attgttcaac gcagttccaa gcatcgccat ttcgttgcgc ccgctgatgt ttgcgcgttg
    20401 ggtaaaatcg cgatggctga cggcactcgc cattgccagc agttgccgcc acggttgtag
    20461 cagtcgcgcc cgcaaccaga taatagtgaa caccagtaaa agtgccataa ataccgccat
    20521 tacccgatgg accagtacca ctgtctcaat gcgcatttcc gtggtgcggt caaaaccaga
    20581 taccagctga tcaagcccgg caacaaactg gctgacatcc gctgacaccg tttctcggtt
    20641 ttgtgcacgc atcagcgcag ggatcagttc attacgccag taatcttgta aaccctgtaa
    20701 ttgcgccagt tgtccgtctc gttctgctgc tcgagtcaac tcggcgctaa atgccgtttg
    20761 ttccatctct ttaattaagg gcttgtcttt ctcacttaat ggcactgctg ccaacagacg
    20821 gtaactttgc atgcgcagcg atcccgcttt gttgatcgca tgggcgctgc cctgaacgcc
    20881 ttgcaccagc cagccagaaa ccgccatccc tgccagtcca atagcagtag aaagcaacac
    20941 aataagcgca acctgattaa ccagggtgag cggagagaga caacgtttaa gcatgtaaac
    21001 ctcttccttc aggctttaaa tgagcaataa ccttaatgaa tgtgacgata cattctggaa
    21061 tggcagtatt ctcggctatt ggctgaagta tacccatacc cggaaagagt tactccttat
    21121 ttgccgtgtg gttagtcgct ttacatcggt aagggtaggg attctacagc accgtgaaaa
    21181 atctcataat ttttatgaaa tcactgtact cactatgggt aatgataaat atcaatgata
    21241 gataaagtta tcttatcgtt tgatttacat caaattgcct ttagctacag acactaaggt
    21301 ggcagacatc gaaacgagta tcagaggtgt ctatgagtca ctcatccgcc cccgaaaggg
    21361 ctactggagc tgtcattaca gattggcgac cggaagatcc tgcgttctgg caacaacgcg
    21421 gtcaacgtat tgccagccgc aacctgtgga tttccgttcc ctgtctgctg ctggcgtttt
    21481 gcgtatggat gttgttcagc gctgttgcgg tgaacttacc gaaagtcggt tttaatttta
    21541 cgaccgatca gctatttatg ttgactgcgc tgccttcggt ttctggcgcg ttattacgtg
    21601 ttccatactc ctttatggtt cctatcttcg gtggtcgtcg ctggacggcg ttcagcaccg
    21661 gtattctgat tattccttgt gtctggctgg gttttgccgt gcaggatacc tccacgcctt
    21721 atagcgtctt catcatcatc tctctgctgt gcggctttgc tggcgcgaac ttcgcttcca
    21781 gtatggcaaa catcagcttc ttctttccga aacagaagca gggtggcgcg ctgggtctga
    21841 atggtggtct gggcaacatg ggcgtcagcg tcatgcagtt ggttgctccg ctggtggtat
    21901 cactgtcgat tttcgcagta tttggtagcc agggcgtcaa acagccggat gggactgagc
    21961 tgtatctggc gaatgcgtcc tggatatggg tgccgttcct tgccatcttc accattgcgg
    22021 cgtggtttgg catgaacgat cttgctacct cgaaagcctc catcaaggag cagttgccgg
    22081 tactcaaacg gggtcatctg tggattatga gcctgctgta tctggcaacc ttcggctcct
    22141 ttatcggctt ctccgcgggc tttgcgatgc tgtcaaaaac gcagttcccg gatgttcaga
    22201 ttctgcaata cgctttcttc gggccgttta ttggtgcgct ggcgcgttct gcaggtggtg
    22261 cattatctga ccgtctgggc ggaactcgtg tcacgctggt gaactttatt ctgatggcga
    22321 ttttcagcgg cctgctgttc ctgaccttac cgactgacgg gcagggcgga agcttcatgg
    22381 cgttcttcgc ggtcttcctg gcgctgttcc tgacagctgg gctgggtagt ggttccactt
    22441 tccagatgat ttccgtgatc ttccgtaaac tgacaatgga tcgcgtgaaa gcagaagggg
    22501 gttctgacga acgtgcgatg cgtgaagcgg caaccgacac ggcggcggcg ctgggtttca
    22561 tctctgcgat tggcgcgatt ggtggcttct ttatcccgaa agcgtttggt agctcgctgg
    22621 cattaacggg ttcgccagtc ggcgcaatga aggtattttt gattttctat atcgcctgcg
    22681 tagtgattac ttgggcggta tatggtcggc attctaaaaa ataaatctta atatttgatt
    22741 atcctttgcg cggcatgatg tcgcgctttt tttatgcgtc atttagttac aacatactaa
    22801 tgttatatgg tttatttcgc cggatttcat taagagccat taa
//
LOCUS       DAPGRN010000073        22767 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPGRN010000073 DAPGRN010000000
VERSION     DAPGRN010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.073"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            149..832
                     /locus_tag="RHW33_003017"
     CDS             149..832
                     /locus_tag="RHW33_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV1646498.1"
                     /translation="MNLKKIFFSAVTVSVLCTLTGCDYIEEGKPESSLLKQQEEHNNK
                     TELIEKQQAQLKSQLETIQKQQTGIITSTKTLTHVIKSVKDQQNTFIFTEFNPAKMKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSIPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            876..4093
                     /locus_tag="RHW33_003018"
                     /pseudo
     CDS             876..4093
                     /locus_tag="RHW33_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            4521..6176
                     /gene="lldP"
                     /locus_tag="RHW33_003019"
     CDS             4521..6176
                     /gene="lldP"
                     /locus_tag="RHW33_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV1646499.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYIAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSVVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6176..6952
                     /gene="lldR"
                     /locus_tag="RHW33_003020"
     CDS             6176..6952
                     /gene="lldR"
                     /locus_tag="RHW33_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV1646500.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            6949..8139
                     /gene="lldD"
                     /locus_tag="RHW33_003021"
     CDS             6949..8139
                     /gene="lldD"
                     /locus_tag="RHW33_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV1646501.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPASLAPMAK
                     GNAA"
     gene            8294..8767
                     /gene="trmL"
                     /locus_tag="RHW33_003022"
     CDS             8294..8767
                     /gene="trmL"
                     /locus_tag="RHW33_003022"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV1646502.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYNAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPVEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(8820..9641)
                     /gene="cysE"
                     /locus_tag="RHW33_003023"
     CDS             complement(8820..9641)
                     /gene="cysE"
                     /locus_tag="RHW33_003023"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV1646503.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKTSGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(9721..10740)
                     /gene="gpsA"
                     /locus_tag="RHW33_003024"
     CDS             complement(9721..10740)
                     /gene="gpsA"
                     /locus_tag="RHW33_003024"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV1646504.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(10740..11207)
                     /gene="secB"
                     /locus_tag="RHW33_003025"
     CDS             complement(10740..11207)
                     /gene="secB"
                     /locus_tag="RHW33_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV1646505.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11270..11521)
                     /gene="grxC"
                     /locus_tag="RHW33_003026"
     CDS             complement(11270..11521)
                     /gene="grxC"
                     /locus_tag="RHW33_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV1646506.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(11663..12094)
                     /locus_tag="RHW33_003027"
     CDS             complement(11663..12094)
                     /locus_tag="RHW33_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV1646507.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12339..13883
                     /gene="gpmM"
                     /locus_tag="RHW33_003028"
                     /gene_synonym="pgmI"
     CDS             12339..13883
                     /gene="gpmM"
                     /locus_tag="RHW33_003028"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV1646508.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLKPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            13893..15176
                     /gene="envC"
                     /locus_tag="RHW33_003029"
     CDS             13893..15176
                     /gene="envC"
                     /locus_tag="RHW33_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV1646509.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15180..16139
                     /locus_tag="RHW33_003030"
     CDS             15180..16139
                     /locus_tag="RHW33_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV1646510.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(16126..17160)
                     /gene="waaH"
                     /locus_tag="RHW33_003031"
     CDS             complement(16126..17160)
                     /gene="waaH"
                     /locus_tag="RHW33_003031"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV1646511.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17399..18424)
                     /gene="tdh"
                     /locus_tag="RHW33_003032"
     CDS             complement(17399..18424)
                     /gene="tdh"
                     /locus_tag="RHW33_003032"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV1646512.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFCIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18434..19630)
                     /gene="kbl"
                     /locus_tag="RHW33_003033"
     CDS             complement(18434..19630)
                     /gene="kbl"
                     /locus_tag="RHW33_003033"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV1646513.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            19844..20776
                     /gene="rfaD"
                     /locus_tag="RHW33_003034"
     CDS             19844..20776
                     /gene="rfaD"
                     /locus_tag="RHW33_003034"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV1646514.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            20786..21832
                     /gene="rfaF"
                     /locus_tag="RHW33_003035"
     CDS             20786..21832
                     /gene="rfaF"
                     /locus_tag="RHW33_003035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV1646515.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLISGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            21836..>22767
                     /gene="rfaC"
                     /locus_tag="RHW33_003036"
     CDS             21836..>22767
                     /gene="rfaC"
                     /locus_tag="RHW33_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV1646516.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPLKSLNNLEAAA"
BASE COUNT         5647 a         5859 c         5974 g         5287 t
ORIGIN      
        1 gaatgataca tttttatgat gaatgctgaa aacaatattt aatcgacacc catcactgtc
       61 attaatgtaa agcaacatag atcaccaata atacaatgga caactctacc ggcattgatc
      121 gctggtcgga ggcaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt
      181 cactgtttca gttctctgca cattaacggg atgtgactac attgaagaag gcaagccaga
      241 aagtagttta ctgaaacaac aagaagaaca taacaataaa actgagctaa ttgaaaaaca
      301 acaagcacag ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataac
      361 cagtacaaag actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat
      421 ttttaccgag tttaatccgg caaaaatgaa gtatttcatc ctgaacaatg gttcggtggc
      481 tttagcgggc cgagtgttat ccattgacgc aacagagaat ggcagtgtta ttcatatttc
      541 gctggtcaac ttattaagta taccaatctc aaacattggt tttaatgcga catggggtgg
      601 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      661 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgacactgaa
      721 gggtgtatcg ccgaataacc tgggatatct gaagttagcc atcaatatgg agaatattca
      781 gtttgacaac cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagct
      841 tctgcgctta tatcaaggaa tagagaacat cattaatgaa caaaatattt aaagttatct
      901 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
      961 aatccgggcg cagtaagctg ttaatttctg ccgtggttgc gggtgggttg ttgtcgtcgt
     1021 ttgaggcact ggcaacaggt aatgaacctg gtacacctgt tggcacagga tctggctggg
     1081 ttgctatagg ccaggaatca caggcaaatg cttttaccga tggtaatggt gccagtactg
     1141 cagtgggtta taaagctttc gctgaaggaa catggagttc cgctattggc gccatgaccc
     1201 atgcaattgg tccggcttca atggcattcg gggttaatgc cgtctcaacc ggtcaacgaa
     1261 gtatcgctat gggcgcatcg tcaagttcag tgggtcaata ttcgatggca cttgggcgat
     1321 atgcgaattc gggtggtgat tattcgatcg cgcaagggga taaatccaat gctgctggtc
     1381 tgaatgccat tgcagtggga agaagcagta gtgcggcagg cgagaaagcc actgcgctgg
     1441 gcaacgcagc taaagctact gagattatga gtattgctct tggcgacagc gccaatgcgt
     1501 caaaagcgta ttcaatggcg ctggtagcaa gtagcgtcgc atctgaagaa aacgcaattg
     1561 ccctggggcg tagcagttag ctagcggtac tgacagcctc gcattaggca gacaaacact
     1621 tgccagtaca gcgaacgcta ttgcgatagg tgctgagagc aaagccgctg aaaatgcgac
     1681 ggccgttggt accaaagcgg aggccaatgg acttaatagt atcgcgttgg gctctggggg
     1741 tattgcagat gccgataaca cgattgctct gggcaatcaa agcctggcag tagctgctgg
     1801 tgcaattgcc atcggtcagg ggaataaagc tgacggcgca aatgctatcg cgctgggtaa
     1861 tggtagtgtt acaggtggcg caaatgctat tgctcttgga caaggtagtt atgccggttt
     1921 agaaaatggc attgcaattg gtggtcaagc cagtgctcaa gggacaaatt ccgttgctct
     1981 gggtgcgggt tctgtagcga ctgaagataa ttccgtctct gtgggtaaca caacagatca
     2041 gcgaaaaatt gtcaatatgg cagcaggtga tatcagcact agcagtactg atgccataaa
     2101 tggatcacag ctttatgcta tcagtaagtc agtagcggac cgtcttggtg ggggggctac
     2161 cgtcaatgcg caaggtgtcg ttactgcccc gaattacaga ctgaaaaatg gtatttatgg
     2221 caatgttggc gatgctttag cggatctaaa caataacacg atacaatggg actccgttaa
     2281 aaagggatat agtgcggcac atggtacaga tgccaccagc accatcacca acgttaaagc
     2341 cggcactatt tctgctacca gtacggatgc ggttaacggt tctcagttga atgcgacgaa
     2401 tatgatgatt gagcagaaca gccaaatcat caatcagctt gctggtaaca ccgacgcaac
     2461 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acggtttagc
     2521 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     2581 gtctggtgcc agcagcgtag ccattggtca gaacagcaat agcaccgttg ataccggtat
     2641 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     2701 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     2761 attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttccga
     2821 agcccatgac gccgttacgg ttcgtcaatt gcagaatgcg attggtgcgg tcgcaaccac
     2881 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     2941 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     3001 gaacactctg gttctggctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     3061 aaaccatgca aacagtattg caatgggtaa cggttctcag accacccgtg gtgcacagac
     3121 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgttgg
     3181 tagtgaagac ggtcaacgtc agatcaccaa cgtcgcagct ggttcagcgg ataccgatgc
     3241 ggtcaacgta ggtcagttga aagtaacgga tgcgcaggtt tcccagaata cccagagcat
     3301 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     3361 tattggcgat atcgtcacca ccggtagcac caagtacttc aagaccaata ccgatggtgt
     3421 agatgccagc gcgcagggta aagatagcgt cgcgattggt tctggttcca ttgctgccgc
     3481 ggataacagc gtcgcactgg gtacagggtc tgtggcaacc gaagaaaata cgatctctgt
     3541 aggctcatct actaatcaac gtcgtatcac caacgtagcc gcaggtaaaa acgacaccga
     3601 tgctgttaac gtggcacagt tgaagtcttc tgaggcgggc ggtgtacgtt acgacaccaa
     3661 cgctgatggt tctgttgact atagcaatat caccctcggt gggggcaacg gcggtacgac
     3721 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     3781 gaagcaaagc gtgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     3841 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     3901 cggtctgccg caggcttata caccgggtgc cagcatggcc tctattggtg gcggtatcta
     3961 caacggtgaa tcagctgttg ctttaggtgt gtcgatggtt agcgccaatg gccgttgggt
     4021 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     4081 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     4141 tgcgctacgc ttatcaggcc tacagggtga ttctgcacat tcctataggc cgggtaaggt
     4201 gttcacgccg catctggcaa gataaggcgc accgggtcaa caatctaagg gcaattctct
     4261 gatgaggatt gcccttttct ttaccagaca tctccccccc acaagaattg gccctaccaa
     4321 ttcttcgctt atctgacctc tggttcacag ttccccaatt aaaactcaca tcaatgttgc
     4381 caatacataa catttagtta accattcatt gtcattatcc ctacacaaca taattggcag
     4441 tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg
     4501 tggactccag gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata
     4561 tttggctttc cagcctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta
     4621 agctcaaact gaaaggatac atcgccgcct catggacagt ggcaatcgcc cttgccgtgg
     4681 ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct
     4741 tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct
     4801 cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata accccagacc
     4861 agcgcctgca aatgctgatc gtcggtttct gtttcggcgc gttcctcgaa ggagctgcag
     4921 gctttggcgc accggtagca attaccgccg cattgctggt cggtctgggc tttaaaccgt
     4981 tatacgccgc cgggctgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg
     5041 gcattccaat cctggttgcc ggacaggtaa caggtatcga cagctttgag attggtcaga
     5101 tggtggggcg gcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta
     5161 tggacggctg gcgcggtatc aaagaaacct ggcctgcggt cgtggttgcg ggcggttcgt
     5221 ttgccatcgc acagtatctc agctccaact tcattgggcc ggaactgccg gacattatct
     5281 cttcgctggt atcgctgctc tgcctgacgc tattcctcaa acgctggcag cctgtacgcg
     5341 ttttccgctt cggcgatctg ggtgcatcgc aggttgatat gacgctggcc cacaccggtt
     5401 acacggcggg tcaggtgctg cgtgcctgga caccgttcct gttcctgacc gccaccgtaa
     5461 cactgtggag tatcccgcca tttaaagccc tgttcgcttc gggcggcgcg ctttatgaat
     5521 gggtgatcaa cattccggtg ccgtatctcg acaaactggt tgcccgtatg ccgccagtgg
     5581 tcagcgaggc tacagcctat gccgccgtgt ttaagttcga ctggttctct gccaccggta
     5641 ccgccattct gtttgctgcg ctgctctcgg ttgtctggct gaagatgaaa ccgtctgacg
     5701 ctatcagcac cttcggcagc acgctgaaag aactggctct gcccatctac tccatcggta
     5761 tggtgctggc attcgccttt atttcgaact attccggact gtcatcaaca ctggcgctgg
     5821 cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg
     5881 tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg
     5941 cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg
     6001 tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg
     6061 tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta
     6121 tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat
     6181 tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga
     6241 aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg
     6301 cgtatcacgt aattcactgc gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct
     6361 cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa
     6421 catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct
     6481 ggaagcccgg tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc
     6541 tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat
     6601 cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt
     6661 gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg
     6721 tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt
     6781 cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct
     6841 tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat
     6901 tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc
     6961 gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat
     7021 atggatgggg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa
     7081 gtggcgctgc gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg
     7141 tttaatgaga aattgtcgat gccggtggcg ctaggtccgg taggtttgtg tggcatgtat
     7201 gcgcgacgcg gcgaagttca ggcagccaaa gcggcggatg cgcatggcat tccgtttact
     7261 ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg
     7321 tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca
     7381 aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc gggagcgcgt
     7441 tatcgtgatg cgcattctgg aatgagcggc ccgaacgcgg caatgcgccg ctatttgcag
     7501 gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc gcatgattta
     7561 ggaaatattt cggcttacct cggcaaaccg accggactgg aagattacat cggctggctg
     7621 gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg
     7681 gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt
     7741 tttggtgctg atgggattgt agtttctaac cacggtggcc gccagctgga cggtgtactc
     7801 tcttccgctc gtgcactgcc cgctattgca gatgcggtga aaggtgatat agccattctg
     7861 gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac
     7921 accgtactgc tgggtcgtgc tttcttgtat gcgctggcaa cagcgggcca ggcgggtgta
     7981 gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg
     8041 aaatcgatca gcgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct
     8101 gcgtcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctccccct
     8161 aaccctctcc ctcagggagc tgtctcttga tcagatctcc tgatcaagag acagggggag
     8221 ggccggggtg aggggaaaag gccgcactgc tcaccaattt tctgctatcc tgcccccgca
     8281 ctaagggggc agtatgctaa acatcgtact ttacgaacca gaaattccgc caaatactgg
     8341 caacatcatc cgtctttgcg ctaataccgg ctttcgtctg catatcatcg aaccgatggg
     8401 atttgcctgg gacgataagc gcctgcgccg cgcggggctg gactatcacg agtttaccgc
     8461 cgttacgcgt catcatgact acaacgcgtt cctcgaagca gaaaatcccc agcgcctgtt
     8521 tgccctcacc acgaaaggta cgcctgctca cagcgccgta agctatcagg atggcgatta
     8581 tctgatgttc ggcccggaaa cacgcggcct gccagcgagc attcttgatg ccctgcccgt
     8641 tgaacaaaaa attcggattc cgatggtgcc agacagccgc agcatgaacc tgtccaatgc
     8701 ggtgtcggta gtggtgtatg aagcctggcg gcagctggga tatccgggag cggtgttgag
     8761 agattagtta cgccatgccg gatagatgat tacatcgcat ccggcacgat cacaggacat
     8821 cagattccat ccccatactc aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc
     8881 attgatggct tatcgctgtc tggtttaccg acaatgcgtg ccggaacgcc agcggcggtg
     8941 gtatgcggcg gcaccggttg cagcaccacg gaacctgcgc caatcttcgc gccgcgccca
     9001 acttcaatat tgccgagaat cttcgcgccc gcgccaatca tcacgccttc acgaattttc
     9061 gggtgacggt cgccgctagt ttttccagta ccgccaaggg tgacggattg cagaatcgac
     9121 acgtcgtttt caatcaccgc cgtttcaccc acaacgatac ccgtggcgtg gtcaagcatg
     9181 ataccgcgac caatttttgc tgccgggtga atatcaacct ggaacgtcac agaaacctgg
     9241 ttttgcagaa agattgccag tgcgcgacgc ccctgattcc acaaccagtg accgatgcga
     9301 taggcctgca aggcatgaaa acccttcagg tataacaacg gggttgagta tttatcgact
     9361 gccgggtcgc gggtacgcac cgcctgaata tcacaggccg cagaggcgat catttccggg
     9421 tcagcggcgt aggcttcttc caccacttca cggatagcaa tagcaggcat aattggcgat
     9481 gacagcttgt tcgccagcat gtagctcagt gcactgccaa ggttttcgtg cttgagtagc
     9541 gtcgcgtggt aaaaactggc cagcattggc tcacagtccg ccagcgttct ggcttcggct
     9601 ttaatattgt tccagacaat ttccagttct tcacacgaca ttgcttactc cacacgatga
     9661 gataatgacc ggcccgttct gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg
     9721 ttagtggctg ctgcgctcgt ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg
     9781 cgcgtttttt ccgcaatata atacttgata aatttcctcg gttattggca tttcaacgcc
     9841 gaagcgatgc gccagttcgc ggacttcttt cgtattgcgg tagccttcca ccacctgacc
     9901 aatcttctcc tgcgcgcttt gtacatccat gccctgaccg agcatcatgc caaaacggcg
     9961 gttacgcgac tggttgtcgg tacaggtaag caccagatcg cccagccccg ccatgcccat
    10021 aaaggtggca ggatcggcac ccagcgccgc gccaagacgc gacatttcag ccaggccacg
    10081 ggtgattagc gccgtacgcg cattcgcgcc aaaaccgata ccgtcggaca tccccgcgcc
    10141 aatggcaatg acgtttttca ccgcgccgcc aagctgcacg ccaatgaaat ccgggttact
    10201 gtaaacgcgg aaacttttac cgcagtgcag cagctgctga agatcatcag caaaagtttg
    10261 gtcggtcgag gccagcgaaa tggctgtcgg taaacctgcc gccagttctt tcgcaaacgt
    10321 tgggccagag ataaccgcca gcggaatttg atcgcctaac gcctcgcggg ccacgtcctg
    10381 taacagacgc ccggtttccg cttccagccc tttggtcgcc cacaccagac gcgcatcagg
    10441 tcgcatcagc ggtttaatct ggcgcagcac ttcaccaaag acatggctgg gtacgacgac
    10501 gagaatatta cggctggctg ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc
    10561 gggaaatggc acatcgggga gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat
    10621 atgttcaggg tcatggcccc agaggacaac ctcgtggcca tttcttgcca gggtgatggc
    10681 aagagcggtg ccgtacgagc cggcaccgat cacagtcatt gaagcattac gttggttcat
    10741 caggcatcct gatgttcttc agtaccttcg ccagcctgct gctgcaaata gttcatgaac
    10801 agcgcatcga agttaaccgg cgcaaggttc agttgcggga atgtaccgcg ggataccatg
    10861 ctggtgatgc actcacgagc atacgggaac agaatgttcg ggcagtatgc gcccaggcaa
    10921 tgtgccatct gggtgccttc gatacccgcg atggagaaaa taccgccctg ctgaacttca
    10981 cacaggaacg cggtttcttc gcccagggag gccgttacgg taacacgcag taccacttcg
    11041 taaacgtcat ctgccagttg agtagatgcc gtatccagat caagtttaac ttctggctgc
    11101 caatctttct ggaaaacgtg cggcgcgttc ggcgcttcga aagaaatatc cttggtataa
    11161 atacgttgga tctggaaagt catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc
    11221 ttaagtgttg tccttaaata ctgcgcaatg taatgccaac gttcacacgt tatttcagca
    11281 ggggatccag tccaccacgt gcatccaatg catacaagtc atcacagccg ccaatgtgct
    11341 gtgcgtcaat aaaaatctgg ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac
    11401 gcttggcggc gttgccatcg atcggcagct cctggaaact cacacccttg ctgctcagca
    11461 gtgcttttgc acgatggcaa tacgggcagg tttctttggt atagatttca acattggcca
    11521 taaattagct cctgtttttt taccctgaaa tttcatgttg cagggcggca gcaaaatgga
    11581 gtgtccccag aagcttataa caataagtca ctgggggcct aattgcagct aacggcctgc
    11641 atcatgaaag acgacaggta aattatttgc cgcgcaccaa aggcaagttt tcgccagccc
    11701 agccagcgac gccttctttc agcacgaata cttgcgcaaa accagctttc gtcagtgcgt
    11761 ttgcaggctc ctggcactgc atgccagaac cgtctaccac gataaccggt ttgtctttgt
    11821 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttaatagaac
    11881 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    11941 ctttgttgat cagacgcgta gcttcaccac gagtaatcac cttcactttc gaggtcaggc
    12001 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    12061 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    12121 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    12181 actggaatgc aattttgcgg ggcacgacga aaaaaaagcg caaatctggc atcggcttgc
    12241 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    12301 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    12361 tggtactggt gattctggat ggctatggct atcgcgaaga acagcaggat aacgccattt
    12421 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    12481 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    12541 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    12601 tcaaagatcg cgctttcttt gctaatccgg tgctgactgg cgcggtagat aaagcgaaaa
    12661 acgcaggtaa agcagtacac attatgggtc tgctctccgc aggcggcgta cacagccacg
    12721 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    12781 tgcacgcatt ccttgacggt cgcgacactc cgccgcgcag tgctgaatcc tcgctgaaaa
    12841 aattcgaaga aaaatttgcc gcgctgggca aaggccgcgt agcgtccatc attggtcgtt
    12901 actacgcgat ggaccgagat aaccgttggg atcgcgttga aaaagcttat gacctgctga
    12961 ctctggcgca gggtgagttc caggccgata ccgccgttgc tggtttgcag gcagcttatg
    13021 ctcgtgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    13081 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    13141 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    13201 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaacc gcggttgctt
    13261 acccaccagc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    13321 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    13381 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    13441 acgatctgaa accggaaatg agctccgcag agctgaccga aaaactggtt gcggcaatca
    13501 agagtggcaa atacgacacc atcatctgta actatccgaa cggcgacatg gtaggtcaca
    13561 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    13621 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    13681 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    13741 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgaaggcggc aaactttctg
    13801 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    13861 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    13921 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    13981 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    14041 ctattcaggc cgatatcgcc gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    14101 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    14161 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    14221 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcacaactgg
    14281 atgccgcatt ccgtcagggc gagcataccg gtattcagct gattctcagc ggtgaagaaa
    14341 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    14401 ccattgccca gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag
    14461 agaaacagag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaagctga
    14521 cccaggcgct gaacgagcgt aaaaagacgc tggcggggct ggaatcttcc atccagcaag
    14581 gtcagcagca gctaagtgaa ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc
    14641 gtgcggaagc cgcggcgaaa gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc
    14701 gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta caaaccgacc gaaagcgaaa
    14761 aatcgctgat gtcccgaact ggtggcctgg gcgcgccgcg tggtcaggca ttctggccgg
    14821 ttcgcgggcc gacgctgcat cgctatggcg agcagctaca gggtgaatta cgctggaaag
    14881 ggatggtgat tggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga
    14941 ttctggctga ctggctgcaa ggctacggtc tggtggtggt ggttgagcat ggtaaaggcg
    15001 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    15061 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    15121 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    15181 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    15241 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    15301 accaggtgct ggcgatgcct tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    15361 ccagagagat ggcgaccaaa gcgcataaca gcggccacga agtgttgatt catctaccaa
    15421 tggcaccatt gagtaaacag ccgctggaga aaaatacgct acgcccggag atgagcagcg
    15481 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    15541 acaaccacat gggcagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    15601 cgctggagcg ttacaatctt tacttcctcg acagcgtaac cattggtaat acccaggcga
    15661 tgcgcgccgc gcaaggcact ggcgtgaagg ttattaaacg gaaggtgttc ctcgacgatt
    15721 cacagaacga agcggacatc cgtgtgcagt tcaatcgcgc aattgatctg gcgcgtcgca
    15781 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    15841 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    15901 cgcaggttga tacgtctaca ccgccgaaaa acactgtgcc cgacacaccg cgtaatccgt
    15961 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    16021 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    16081 agtggcaagg ctggggcaaa cagcccgaag cggcgaaact taacgctagc gcaaattaaa
    16141 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    16201 agaccacagt aacgcctggt aacccacctt cacgctgcgc acattggtaa tcaggcgctt
    16261 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    16321 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    16381 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    16441 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    16501 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcatt
    16561 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    16621 gttattttta acaataacat cgcggcgata aacccccatc cagacaacgt gagtccaacg
    16681 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacaccgg ttgagcgaag
    16741 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt
    16801 gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc atcagcgttt cgtacatggt
    16861 gggatagact tcatcgtcag catcgacaaa agcgacatat ttgcccgttg ccacttcaat
    16921 cccacgatta cgcgccaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    16981 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    17041 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    17101 atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc tgttcatcat
    17161 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    17221 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    17281 gagagcagct atataagaga tcagcacaat tacgaactct ttattacact gacaggattt
    17341 tacatattta acagattatc ctcagataaa agggctggaa taccagccct tgttcgtgtt
    17401 aatcccagct cagaataact ttcccggact ggcccgaacg catagcgtca aagcccttct
    17461 ggaaatcatc gatacagaaa cgatgggtaa tgatcggcga gagatcgagg ccagactgaa
    17521 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaatg cctttaatga
    17581 acaagccctt aaagatcact tttgtccagt ctatcgacat gtcagaaggt ggaataccca
    17641 gcatcgcaat acgaccgccg tggttcatgg tgtcgagcat ggtacgaaac gctggcggcg
    17701 caccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct gccatcacat
    17761 cattaaggtt ttctttagcg acgttgaccg cacgggtgat acccattttg cgcgccagtt
    17821 cgagacgata ttcattaaca tcagtgatca ccacattgcg tgcaccaacg tgtttcgcca
    17881 ccgccgcagc cataatacct atcggacctg caccagagac cagcacatcc tcgccaacca
    17941 gatcgaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt
    18001 cgtcggagat attgtctggg attttgaagg cgttgaacgc cgggatcacc agatattcgg
    18061 cgaagcagcc cggacggtta acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac
    18121 cgccacgaca gttgcgacag tgaccgcaag tgatatggcc ttcgccagaa acgcgatcgc
    18181 caatcttgaa gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    18241 ccacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    18301 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    18361 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    18421 tcatctcaca tcctcaggca ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    18481 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    18541 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatcccctct ttttgcagct
    18601 cacgagcaaa tttctgcgcc actaccgcat caccaagcat gaccggaata atggcgtgat
    18661 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactga cgcgcgttcg
    18721 cccacagacg atcacgcagc tcgctacccg cttcgaccat ctccagcact ttgatggatg
    18781 cggcaacaat ggccggtgcc agcgagttgg agaacagata cggacgagaa cgctggcgca
    18841 gccactcaac cacttctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    18901 taccaagcgt accggtgata atatcgaccc ggcccatcac atcgcagtat tcatgggaac
    18961 cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat
    19021 cgtatttatc tgccagatcg caaacgccct tcagattggc aatcacgccg tccatcgaga
    19081 acacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    19141 gagcttcaag ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac
    19201 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    19261 ccagcagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    19321 cttccatccc caggaatgcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    19381 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgccg
    19441 ccgcaatcag atccggatga ttcgccagcc cgagatagtt attggcacaa aagttaatga
    19501 cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    19561 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    19621 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    19681 ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata acgtccatca
    19741 atctcacttt tggcgaatgg ctgaagccaa cgccgtgtag tgatatgata gaagtattcg
    19801 tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca tcgttaccgg
    19861 cggcgcgggc tttatcggca gcaacatcgt taaggccctg aatgataaag gcatcaccga
    19921 tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg tggatctgga
    19981 tatcgcggac tatatggata aggaagactt cctgatccag attatggctg gcgaagagtt
    20041 cggcgatgtc gaagcgattt tccacgaagg cgcatgctct tccaccaccg agtgggatgg
    20101 caagtatatg atggataaca actatcaata ctccaaagag ctgctgcact actgtctgga
    20161 gcgcgaaatc ccgttcctgt atgcatcttc cgcggccacc tacggcggac gcacctccga
    20221 ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact caaaattcct
    20281 gtttgatgaa tatgttcgtc aaatcctgcc tgaagcgaac tcgcagattg ttggcttccg
    20341 ttatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt
    20401 ccatctcaac acccagctta acaacggtga atcaccgaag ctgtttgaag gtagcgagaa
    20461 cttcaaacgc gacttcgtct atgtgggcga cgtggctgat gtgaatctgt ggttcctgga
    20521 aaatggcgtt tccggcatct tcaatctcgg tactggtcgt gcggaatcct tccaggctgt
    20581 agctgatgct acgctggctt atcacaagaa aggccagatc gaatacattc cgttcccgga
    20641 taaactgaaa ggccgctatc aggcgttcac tcaggcagat ctgacgaatc tgcgcgcggc
    20701 gggttacgac aaaccgttca aaaccgttgc tgaaggtgta acggaataca tggcctggct
    20761 gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt cttgggttgg
    20821 cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc cccaggcgat
    20881 aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc cggaagttaa
    20941 cgaagctatc cctatgcctc tcggtcacgg agcgctggaa atcggcgaac gccgcaaact
    21001 gggtcatagc ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccca actccttcaa
    21061 atctgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc gcggcgagat
    21121 gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc cgctaatggt
    21181 ggaacgctat atagcgctgg cctatgacaa aggcattatg cgcacagcac aagatctgcc
    21241 gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa
    21301 tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg cggagtttgg
    21361 tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc tgattgatga
    21421 aggttatcag gtggttctgt ttggctcggc gaaagatcat gaagcgggca atgagattct
    21481 tgccgctttg aataccgagc agcaggcatg gtgtcggaac ctggcggggg aaacacagct
    21541 tgatcaagca gttatcctga ttgcagcctg taaagccatt gtcactaacg attctggcct
    21601 gatgcatgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc cgagtagccc
    21661 ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgtttgatta gcggctatca
    21721 caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttaatcg acattactcc
    21781 ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct gacggatgcg
    21841 ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc ccgcactcac
    21901 tgatgcccag caggcaatcc cagggattaa atttgactgg gtggtggaag aagggttcgc
    21961 acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg
    22021 gcgtaaagcc tggttctcgg cccccataaa agcggaacgc aaagcgtttc gtgaagcgct
    22081 acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa gcgcggcgct
    22141 ggtgacgcgt ctggcgcatg gcgtaaagca tggaatggac tggcaaaccg ctcgcgaacc
    22201 tttagccagt ctgttttaca atcgtaagca tcatattgca aaacagcagc acgccgtaga
    22261 acgcacccgc gaactgtttg ccaaaagtct gggctatagc aaaccgcaaa cccagggcga
    22321 ttatgctatt gcacagcatt tcctgacgaa cctgcctaca gatgctggcg aatatgccgt
    22381 atttcttcat gcaacgaccc gtgatgataa acactggccg gaagaacact ggcgagaatt
    22441 gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg cgccgcatga
    22501 ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat tgccgaagat
    22561 gagtctggaa ggcgttgccc gcgtgctggc cggggctaag tttgtggttt cagtggatac
    22621 ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt atggacctac
    22681 cgaccctgga ttaattggcg ggtatgggaa gaatcagatg gtttgtaggg ctccgctaaa
    22741 gagtttaaat aatttagaag cagcggc
//
LOCUS       DAPGRN010000074        22756 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPGRN010000074 DAPGRN010000000
VERSION     DAPGRN010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.074"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..1038
                     /locus_tag="RHW33_003037"
     CDS             16..1038
                     /locus_tag="RHW33_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HDV1646517.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASLTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFAASPAANLRFYAARGPRGLETGLKGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWHGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1038..2018
                     /locus_tag="RHW33_003038"
     CDS             1038..2018
                     /locus_tag="RHW33_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDV1646518.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYNLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACTA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEAHSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2015..2773
                     /locus_tag="RHW33_003039"
     CDS             2015..2773
                     /locus_tag="RHW33_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1646519.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEVLLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISH"
     gene            2783..3595
                     /locus_tag="RHW33_003040"
     CDS             2783..3595
                     /locus_tag="RHW33_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV1646520.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLISKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGIDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVRWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQEALR"
     gene            3592..4446
                     /locus_tag="RHW33_003041"
     CDS             3592..4446
                     /locus_tag="RHW33_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HDV1646521.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CASGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAIQNVLEW
                     SCGVSDYLDQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            4472..6442
                     /locus_tag="RHW33_003042"
     CDS             4472..6442
                     /locus_tag="RHW33_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV1646522.1"
                     /translation="MRLKKHYLCTALSLAFTQQAVAAQESDSLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEASLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGNHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFVNEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(6492..6746)
                     /locus_tag="RHW33_003043"
     CDS             complement(6492..6746)
                     /locus_tag="RHW33_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV1646523.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMSGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRG"
     gene            6947..7681
                     /locus_tag="RHW33_003044"
     CDS             6947..7681
                     /locus_tag="RHW33_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV1646524.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     VITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AGLHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(7683..8294)
                     /gene="emtA"
                     /locus_tag="RHW33_003045"
     CDS             complement(7683..8294)
                     /gene="emtA"
                     /locus_tag="RHW33_003045"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV1646525.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            8394..9308
                     /gene="ldcA"
                     /locus_tag="RHW33_003046"
     CDS             8394..9308
                     /gene="ldcA"
                     /locus_tag="RHW33_003046"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV1646526.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNTFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAMLNNTHEGTQLTISGHPVLKM"
     gene            9403..11139
                     /locus_tag="RHW33_003047"
     CDS             9403..11139
                     /locus_tag="RHW33_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV1646527.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            11260..11421
                     /locus_tag="RHW33_003048"
     CDS             11260..11421
                     /locus_tag="RHW33_003048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646528.1"
                     /translation="MDDKPLQALANKLKTPAQESQRNGFFRLQQVKNNQPRITGIPVN
                     HQGPQPDIA"
     gene            11760..13196
                     /locus_tag="RHW33_003049"
     CDS             11760..13196
                     /locus_tag="RHW33_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646529.1"
                     /translation="MASFKDICPFAPAIPPSSVNTGVRRPPRRLFLSVELKNQILQNM
                     KYLSPQKFSWGDAPWQIIDLSIAGKVNIQVDNNTIITLGTPLNPQHNEFMMVAEWCQW
                     AMEREGLQENLQKKLHDVLEENRQNKQSEIPQEDLKERLKEIKEDILEENQSASQIED
                     RAEALRRMKECLITRQSMLDLSNLGLTSLPENLPPHLIEFNCSRNVLTALPEVMPKGL
                     RVLECMENFLILLPKVQPPKLMVLKCYENYIIWLPELSTNLRVINCSENFLQFLPPSM
                     PQYLYKLSCAGNNINSIPDEMLENLTRLKVFDCSSNDLISSPRLPPKLIIYYCGENKF
                     KTVQVPQPQSLKVFDCNGNPWDKDNLPTLLKAVEGLKKQQGLKDLLGFLHKEGQMKLE
                     KLEKLEDLDDLMDLEFLDDPELLERVKVQEDLELLNQQLGLLSLERQQDSQPVNQQSE
                     HEPEPASRVKRDLSEVEFESTMKRKRFM"
     gene            complement(13365..14435)
                     /gene="dadX"
                     /locus_tag="RHW33_003050"
     CDS             complement(13365..14435)
                     /gene="dadX"
                     /locus_tag="RHW33_003050"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV1646530.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCSLALRVPVVTV"
     gene            complement(14445..15743)
                     /gene="dadA"
                     /locus_tag="RHW33_003051"
     CDS             complement(14445..15743)
                     /gene="dadA"
                     /locus_tag="RHW33_003051"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV1646531.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVCLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESCRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVTF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            16073..17605
                     /locus_tag="RHW33_003052"
     CDS             16073..17605
                     /locus_tag="RHW33_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV1646532.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKFYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            complement(17657..18376)
                     /gene="fadR"
                     /locus_tag="RHW33_003053"
     CDS             complement(17657..18376)
                     /gene="fadR"
                     /locus_tag="RHW33_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV1646533.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            18598..20139
                     /gene="nhaB"
                     /locus_tag="RHW33_003054"
     CDS             18598..20139
                     /gene="nhaB"
                     /locus_tag="RHW33_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV1646534.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            20285..20815
                     /gene="dsbB"
                     /locus_tag="RHW33_003055"
     CDS             20285..20815
                     /gene="dsbB"
                     /locus_tag="RHW33_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV1646535.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(20861..22129)
                     /gene="umuC"
                     /locus_tag="RHW33_003056"
     CDS             complement(20861..22129)
                     /gene="umuC"
                     /locus_tag="RHW33_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV1646536.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(22129..22548)
                     /locus_tag="RHW33_003057"
     CDS             complement(22129..22548)
                     /locus_tag="RHW33_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV1646537.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         5435 a         5970 c         5737 g         5614 t
ORIGIN      
        1 atcaggatat aacttatgtc actaaccaga cggaggttta cacagattct tgcgtcgacg
       61 ttgttcctgc atcatctgcc ttcttttgca caatcagtca aattctgggc ctcactgacg
      121 cttcccgaag cccaaaacat tacacgcatc gtcagcgcag gtgcgcccgc cgatttgcta
      181 ttgctggctg tcgcgccaga aaaaatggtc ggtttttcct cttttgattt tgcccgtcag
      241 gcattaattc cattgccaga gcacattcgc cagttaccca ggctgggacg actcgccggg
      301 cgcgccagca cactctcgct ggaagggctt atggcgttac atcccgattt ggtcgttgat
      361 tgcggcaata cggatgaaac ctggatctcc caggcacggc aggttagcga acagacacaa
      421 ataccctggt tattgcttaa cgggaaactg gcgcaatcag cagaacagtt aacaacgctt
      481 ggcaaaacgt taggcgaaga gcatcgcgcc gcagaacaag ccaatctcgc cagccgcttc
      541 gttggtgaag ctcaggcatt cgccgcctca cccgccgcta acctccgctt ttatgctgcg
      601 cgcggtcctc gagggctgga aacgggctta aagggttcgt tgcataccga ggcggcggaa
      661 ttattaggtt tgcacaacgt cgcgcaaata gccgatcgcc acggcctgac acaggtttcc
      721 atggaaaatc tcctgcgctg gcagccggat attattctgg ttcaggaggc cgttactgca
      781 gattttattc gtcgtgatcc tctctggcat ggcgtgaaag ccgttgcgga acaacgcatc
      841 ttatttttaa gtggcttgcc ctttggctgg ctggatgccc caccgggaat caaccgtctt
      901 ctgggattac gcagacttca cgcctggctg gatcccgcca tcaatcgcca gtttaaaagt
      961 gacatgcagc attacgccca actgttctgg cattgttcac tcagtgacgc cgactatcaa
     1021 aaattggtgg cgagctaatg agaatcgtaa acggatgtat tttgctgctg gcagctatca
     1081 gtatcacttt tgccgccgta agtggggctt ataatctcga tatgcagcaa ctgctggcgc
     1141 taattcttcg tcaagaaaat gttcctgtgc aggaacaaat tgtcttctgg caaatccgcg
     1201 tgccgcgtat tcttgctgcg ctgttactgg gcgcggcgct ggcaggagcc ggaaccacct
     1261 atcagggaat gttgcgtaat cccttagttt caccggatat tttaggcgtc tccgctggtg
     1321 ccggacttgg tgcctgcacg gcaattctgt ggggattatc cattgtttac atccagctgt
     1381 acgcattttg cggcgggctg atggtggtcg cgggcgtctg gttgattacg cgtcgggtga
     1441 cccgccacga tccgatctta accctggtgc tggtaggcat tgcgttagga acgttgtgcg
     1501 gagcggggat atcgctaatt aaaacgctgg ccgatcctta cacccagtta ccgtcaatta
     1561 ccttctggtt gctgggggga ctgtccaccg tgaccttacg cgatctctgc tatgctgcac
     1621 cgatcattct cataggctcc ttgcccctgt tttttctccg ctggcgcatg aatttactca
     1681 cgctttccga cgacgaagcc cattcgctgg gactgaacgt gacgcgtctg cgctttggat
     1741 tgattgtctg cgccacacta atcaccgcca gtacggtagc aatagcgggc ataattggtt
     1801 gggtcggcct ggtcgttccg cacattgccc gcttgttgac cggtcataac catcagcagc
     1861 tgttgcccat ggcaatgtgt actggcgcga ttctgctcct gcttaccgat acgctggcac
     1921 gcagcattgg aacaacagaa atcccacttg gcattctgac cgcttttgtt ggcgcaccgt
     1981 ttttcctgct tttgttgtta cgcggaggtc gccagtgact ttgcttgccg tccgtcatgc
     2041 gtctctgggt tactcccgcc atccggtgct acgcgacgtg tccttcaccc tgtcgcaagg
     2101 aacaatttgt tgtctgttag gagccaatgg ttgcggcaaa accacactca tgcgctccat
     2161 tcttggcgtt ataccgttat taaaaggcga ggtcttactg gatagcctcc ctgtgcagac
     2221 actcagccat cgccagcgcg cgcaggctat tgcctgggtg ccgcaggcgc acgacggtat
     2281 ttttgctttt agtgtgctgg atatggtgtt gatgggcctg gcccccacca taggcgcttt
     2341 ttccgttccc ggtaagcaag aacgcctcaa agccacggag caactggaaa agctgggtat
     2401 tttgcatctg gcggcacgac gctggaatac gcttagcggc ggtgaacgtc agctggcttt
     2461 aatcgcccgt gcactcgtcc agcaaccgcg cttgttgtta ctggatgagc ctgcctccag
     2521 cctcgatttc ggtcatcaga tccaactact ggacacgctg gcacagctca aaaataacgg
     2581 catgacaatg ttaatgtcga ctcatcatcc gctccatgcc aacgccattg ccgacagcat
     2641 tatccaggta gagccagatg gacgagtaac acaaggctta ccgaccgaac agttaacaac
     2701 aaacaaactg gctgccctgt atcgggtttc tgccgaccag attcatcatc acctctctgc
     2761 aattagccac taaggaacaa tcatgctcat tgatgatatt gattttgcag acctctacct
     2821 gcagcaactc aaactggccc accgcacaga aaaaacgccg gatcactggg atcaacgggc
     2881 ggaaaaaatg gcggaaaatt gcgcctcgcc tacggacagc tacctgcaac aactgatctc
     2941 aaaaattgac ctacaaggcg ctcagacctt attcgatatg ggctgcggac cgggtaccgt
     3001 atcgctggcg ctggctgaca aactcacgac gatttatggc attgattaca gtcaggggat
     3061 gctgaacgtt gccgcccgtc gcgccgccgc gctgaaggca gacaatgtcc actggatcca
     3121 gcgcgcctgg gaagaggact ggagtgacct gccacgctgc gatattgccg tggcttcccg
     3181 ctccactctg gtggctgata tgcgccaggc aatgagcaaa ctgaataatc aggcacggct
     3241 tcgcgtctat acgactcatc tggtcagcac ctcgttcgtc tctccagcca ttcagcgggc
     3301 ggctggtcgg gaagtgatcg aattacccaa ctatatcttt gcgctcaacg tcctgtatca
     3361 gatgggaatt tatgcccacg ttgattttat ccgtggacaa aattgccaac aggataacag
     3421 cacctgggaa cgttttgagc agaacgtcag atggagtctt ggcgcgctta acgacgatga
     3481 gcgtgagcgg ttatatcgct ggtatcaaca acaagatgct cgtgccctcg cccctgccag
     3541 ccgcgactgg gcgctcatct ggtgggatag cgtaccgcag gaggcactgc gatgattttc
     3601 ctttctcagg cacaaatcga tgcgttactt ctggaagata tccagggcgg tgacctgacc
     3661 acgagagcat taaatattgg acaccagcat ggctatatag agttttttct ccgtcagggc
     3721 ggctgcgcca gcggcgtttc tgtcgcgtgt aagatgttaa ctacgctggg attaactatt
     3781 gatgacgcgg tcagcgacgg ttcacaagcg aacgcgggtc agcggctaat ccgtgcgcag
     3841 ggtaatgccg cagcacttca tcagggatgg aaggcaatcc agaatgtgct ggagtggagt
     3901 tgcggtgttt ctgattatct cgatcaaatg ctggcgttac ttcgtgaacg ttatcctgat
     3961 ggcaatatcg cctgcacccg aaaagcaatt ccgggcaccc gcttactggc ctcgcaggca
     4021 attctggctg ccggaggact gattcatcgt gccggatgtg cggaaaccat attattgttt
     4081 gccaaccacc gccattttct tcatgacaat caggactggt caggcgcaat caatcagtta
     4141 cgtcgccacg cacccgagaa gaaaattgtt gttgaagccg acacgccgaa agaggcaatc
     4201 gccgcgttac gcgcgcaacc agacgtactt cagctcgaca aatttagtcc gcaacaggca
     4261 actgaaattg cgcaaatagc gccgtcgctg gctccccact gcacgctggc tctgaccggc
     4321 ggaattaatc tgacgacact caaaaattac ctcgactgcg gcattcgcct ttttattacc
     4381 tccgcgcctt attacgcggc accggctgac attaaagtca gtctgcaacc cgcagcctct
     4441 atttaattct ttgttaaacg gaatttattc tatgagactc aaaaaacatt atctctgcac
     4501 agcattatca cttgccttta cccagcaggc cgtagcggct caggagagcg actcgctgac
     4561 cgtatggtcc agtccggtat catcgacgac gaccaccgtt ctcgatcaac ccaccatgaa
     4621 ggccctggat aaacagaatg tcgctcaggc attaagtgtc gtccccggcg tggtgctgca
     4681 aaagtcaggc agccgcaacg aagaacaggt aaaagttcgt ggctttgata gtcggcaggt
     4741 gccggtctat ttcgacggtg tgcccattta tgttccctat gacggcaacc tcgatctggc
     4801 gcggattctg accaacaatc tgggggcagt cgaagtttcc aaagggtatt cgtcgctgct
     4861 tcaggggcct aatcagatgg gcggagccat taatatcacc acccagaagc caacaaaacc
     4921 tctggaagca agtctgggat atcgccaggg atggagccgt agccaggaca atgcctacga
     4981 tatgcatgct tcatttgccg ccagcagcga tctggggtat ttgcaagtca gcggtagcca
     5041 gctaaagcag gattttctcg gcctgccgca tggtgtaaat aatgatattg caggcaacca
     5101 cggcaagatg attaattcat cggctgatga taaacgcggc attgtgaagc tcggatttac
     5161 accacgtgaa aacgatgaat acacactgac ttacattaag caggatggtg aaaaagataa
     5221 cccgccatac agcggaaata gtggtcaaaa atcacgctac tggcagtggc cagagtatga
     5281 caaagaaagt ttttattatc agggaacgac ccaactaaac gatcgtttta ccctgaaaag
     5341 tcggctgtat cgcgacacct ttgaaaatac gctgatgatg tacaactcgc tggctgattt
     5401 gaaaaataaa aaaggcagct acagccatta ttccgattac agcgacggtg ccggattaca
     5461 actggcagcc gatgtgcgtg aaaacgatct gctgtcgttt gccgttaact ggaaagatga
     5521 cgtacaccgg gaaaaaggtg cgccgcacgc cgcttacgat cgctatgaag atcgtacctg
     5581 gtcgctcgcc agtgaatatc aatgggctgc tgccgataat gtcgatgttg tggctggaat
     5641 cagctatgac tggcgcgata gcgtagaagc gaaaaaacac gagaaagatg gcagcatcac
     5701 ccattatgac gacaacaatc agtcagcttt taactggcag gtgatgggga aataccactt
     5761 tgtcaatgaa gacacgctgg cgctttcgta ctatgaccgc acacgctttc cgacgctgaa
     5821 agaacgctat accacgtcca aacctgcgta taaccagata gcgattgtta acccgcagct
     5881 caagccggaa cgcgctcgcg gggtggattt aacctggaat ggtgccttca cgcacgactg
     5941 gggatttgag gtcagcgttt actataaccg ggtgagtgat gccatcctct cgcacaatat
     6001 cgatgccgat accattcaaa atcagaacag cggcacggtg gattacagcg gtctggatgc
     6061 cggtattaag gggaaaatca gcaatatact ggatgtagga ttgagctacg ccctgatcca
     6121 cgctgacgcc aaacgtaaag acatcggcaa gataaccgat ctgccaacgc agacaatgac
     6181 cgcatggatg actctcaaac cgtgggagcc gttaagcgta acgctgtcgg aagaggcgcg
     6241 ttcctccagc tacagtaaca gtgacggttc acaaaaagcc gccggttttg cggtgaccca
     6301 cattcgagcc gattacacct taggtcatgg cttcagcgtc aatgcgtcgg tcaataacct
     6361 gtttgatacc caatatgcct acagtgaagg gtttattgaa gaaggtcgca atttctgggc
     6421 aggtgtcgag tatacgttct gatgtgaaag cggatagcgg catctgtatc gctatccgcc
     6481 ttatgaaaat cttatcctct tcgcaggagg cggaatatgc ccagaacgag aatagctccc
     6541 accaccgcca ccaggaagct gtgcaaatta aaaccgctga tggagccgcc aatgccaaac
     6601 atggtcgcca gccagccgcc gaccaccgca ccgactatcc cgagaataca ggtcaggaaa
     6661 aatccaccac catcacgccc cgacatgatt agcttggcga taatgccggc tatcaggcca
     6721 aaaataatcc aggcaataat tcccatgttc aggccctcgt ttcgatgaat cggacgcaag
     6781 aaatgcttac gtcaacgctc taagtatagg caagggcggg atcgatgctg ggcgtttgtc
     6841 ggctgattta agaacaacat gtaaaaaata ttcacgcatt tgtattaagt tttgttaact
     6901 gtgaccgata aaccaaagac agtttgtcag tcaggagttt ttccgcgtga gtcattacca
     6961 tgagcagttc ctgaaacaaa atccgttagc cgtcctgggc gtgttacgcg atttgcacaa
     7021 agccgcaatt cctttgcgta tcagctggaa tggcgggcag ctgatcagca aaatactggt
     7081 aataaccccg gataaactgg tgctggattt cggcagtcaa gccgaagaca acatcgccgt
     7141 gctaaaggca cagcacatta ccattaccgc cgaaactcag ggtgcgaaag tcgagtttac
     7201 tgttgaacaa ctacaacaga gtgaatactt gcagcttccg gcatttatta ccgtaccgcc
     7261 tcccacctta tggtttgtac aacgacgccg atatttccgc atctccgccc cactccatcc
     7321 gccttatttt tgccagacca aactggcgga taacagtacg ttacgtttcc gcctgtatga
     7381 tttgtcgtta ggcggcatgg gcgcattact ggaaacagca aagcctgccg ggttgcacga
     7441 aggtatgcgc ttcgctcaga ttgaagtcaa catggggcaa tggggtgttt ttcactttga
     7501 cgcccagtta atctccatca gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac
     7561 cactccccgt ctgagcttcc gttttcttaa cgtcagcccg acggtggagc ggcaattaca
     7621 gcggattatt ttctctctcg agcgggaagc ccgggagaaa gcggacaaag tacgcgactg
     7681 atttacatcg cgtccagtgc ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca
     7741 ggatgatttc gcgctacgtg atcgaggaac tcgtcagcat ccagatcgtt gattttgctg
     7801 attgcctttt tccgatctga cgagaaagtc cgtagcagcg cacctgcccc gttagcatat
     7861 gacaccacca gcgcatattg cagtaccttc ggatcttcaa tgcctgccag cgggccggtt
     7921 tccagaatat tcaggtaagc cgcccccata gaaatattac gctccggatt tttcagctcg
     7981 ctggtcgtcg gctcaccact ccagcccata cgccgataaa catcacgtcc ggaggttgaa
     8041 gcttttaact gcatcaaacc aatggcattc gatttactca ccgcgttcgg attaccaccc
     8101 gattcgatag cgataatcgc cgtgatcaat tgtggatcga cgccccaggc tgcaccggct
     8161 ttctggctta ttggcatcca ctgcatcgca cgttgcaccg gaactttcgc gttccacggc
     8221 gggttcgtat agtcatgctt tgatgaacaa cccgctaata acacaatcaa aaaggcaaac
     8281 catctcaatt tcacttcttc tatccttata gcctggaatc gccgttgagg tgacaacggc
     8341 acacctaagc ggcatgatat acatttcgtt tctgttgtca gcaaggaatt gccatgtctc
     8401 tgtttcactt aattgcccca tcgggttact gcattaaaca gcacgccgcg ctgcgtggta
     8461 tccagcgcct taccgacgcg gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt
     8521 gtgagcgttt tgcgggtacg gaaacggagc gtctggaaga tctcaattcc cttgccagac
     8581 tgaccacccc caacaccatc gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac
     8641 tggcagatat tgactggcag gcactggtgg ctcgccaaca atatgacccg ttgctcatat
     8701 gcgggcatag cgattttacc gccattcagt gcggtcttct ggcgcaaggc aatgtcatca
     8761 cctttagcgg ccccatgctg gtagcaaact ttggcgcgga tgaactgaac acctttaccg
     8821 agcaccattt ctggctggcg ttacgcaatg aaacctttac cattgaatgg caaggggaag
     8881 gcccgacatg tcgggccgaa ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac
     8941 tgatcggtac accgtggatg ccaaaaattg agaacggtat tctggtgctg gaagatatta
     9001 acgagcaccc tttccgcgtc gaacgtatgc tgttgcagct ctatcatgct ggaattttgc
     9061 cacgtcagaa ggcgattatt ctcggtagct ttagcggcag tacgcccaat gattatgacg
     9121 caggttacaa cctggagtca gtgtacgcgt ttctgcgttc ccgcctgtcg attccactca
     9181 ttaccggcct cgattttggt catgaacaac gcacggttac gctgccgttg ggcgcacatg
     9241 ccatgctgaa taacacccat gaaggcactc agttgacgat tagcggtcat cctgttctta
     9301 aaatgtaaaa aaatcacgct tcagggctaa gtaaaacgct gtctctgccg ctaatatggt
     9361 agggtttata taagagacag cgtaatcagg agtaaccgac cgttggatgc cacaacaata
     9421 attagccttt tcatcttagg atccatcctt gtcaccagca gtatattact tagttcattt
     9481 tcttcccgtc ttggcattcc tattctggtt atttttctgg cgatcggcat gctggcggga
     9541 gtcgatggcg tcggcggtat cccgtttgat aattacccct tcgcctacat ggtaagtaac
     9601 ctggcgctgg cgattatttt gctcgacggc gggatgcgca ctcaggccag ttcctttcgt
     9661 gtggcgttag gaccggcgct gtcgctggcg acgctgggcg tacttatcac ctctggttta
     9721 accggcatga tggcggcgtg gctgtttaat cttgatttga ttgaaggctt attaatcggc
     9781 gctatcgtcg gctccaccga tgctgcagcg gtcttttctc tgctgggtgg taaggggctt
     9841 aacgaacgtg ttggctcgac gctggaaatt gaatccggca gtaatgatcc aatggcggtc
     9901 tttctgacga ttaccctgat tgcgatgatc cagcaacatg aaagcagtgt cagctggatg
     9961 ttcgtggttg atattctgca gcaatttggc ctcgggattg tcatcggtct tggcggtggt
    10021 tatttactgc tgcaaatgat taatcgcatc gccctgcccg ccggtttata tccattgctg
    10081 gcattaagcg gcggtatttt aatttttgct ttaaccacgg cgctggaagg tagcggtatt
    10141 ctggctgttt atctgtgcgg ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc
    10201 atcctgcaaa atttcgacgg cctcgcctgg ctggcgcaaa tcgccatgtt cctggtgctg
    10261 gggctgttgg ttaacccaag cgatctgctg cctattgcca ttccggcgct cattttgtcc
    10321 gcatggatga ttttcttcgc ccgtcctctt tcggtatttg ccggattgct accattccgt
    10381 ggcttcaatc tgcgtgagcg cgtgtttatc agctgggtgg gattacgcgg cgcggtgccg
    10441 attattctgg ccgtgttccc gatgatggcg gggctggaga atgcacgtct gttctttaat
    10501 gtcgccttct ttgtggttct ggtttcactg ctcttgcagg gaacatcact ctcgtgggcg
    10561 gcgaaaaaag ccaaagtggt ggttccgccc gtcggacgtc cggtgtcacg cgttggcctg
    10621 gatattcatc cggaaaatcc gtgggaacag tttgtttatc aattgagtgc tgataaatgg
    10681 tgtgtaggtg cggcactgcg cgatttgcat atgccaaaag agacgcgtat tgcagccctg
    10741 tttcgtgata accagttgct tcatcctacc ggcagcaccc gactgcgcga aggcgatgta
    10801 ttgtgtgtaa ttggtcggga acgcgatctc ccggcgctcg gtaaactgtt cagccagtcg
    10861 ccaccggtcg cgctggatca acgcttcttt ggtgacttca ttctcgaagc cagcgccaaa
    10921 tatgcagatg tggcgctgat atatggcctg gaagacgggc gagaatatcg tgataaacag
    10981 caaacgctgg gtgaaattgt ccagcagttg ttaggcgcgg caccggttgt cggtgaccag
    11041 gtagagtttg ccggaatgat ctggacggtg gccgagaaag aagataatga agtgttgaag
    11101 attggcgttc gagtggctga agaggaagct gaatcttaaa tttaatgaag catctatcgc
    11161 cggttacgat gctttgctta acgcagcctg agcctgtata taaacttgtc atttcctgat
    11221 tttgatatgc acattccaac atcagaagaa ggttaaatta tggatgacaa accattgcag
    11281 gctctggcca ataaactcaa aacaccggct caagaaagtc agcgtaatgg cttcttccga
    11341 ctgcaacagg tgaagaataa ccagccccgc attaccggta tccccgtcaa ccatcagggg
    11401 ccccagcctg acatcgcctg aacatattcc cccacgcaac ctgccgcgct gacgtagccc
    11461 acgcaatacg cgtttcatcc gcctatagca ccggatgcat gagcgatttc ttatagttat
    11521 tcctttgctt aataacgatt atttccgggt cgttcgctct cgtattttct ttaatacgtc
    11581 agagctacct gggccgtaat acagcatgta ccagacccga cacggcattt ctgaccaggg
    11641 aaaaagtcaa cgatgagtag ttatatcagc gcaaatgacg tcctgtggct cctttgcggt
    11701 gaccacattc acgtctccgg cgctgccaga ggatatttat ttcaaggata cgtagcatta
    11761 tggctagctt taaagacatc tgcccattcg cccccgctat tcccccatct tccgttaaca
    11821 caggcgtacg acgcccgcca aggcggctct tcctgtcggt ggaactcaag aatcaaattt
    11881 tgcagaacat gaagtatctc agcccgcaaa aattcagctg gggtgatgcc ccctggcaga
    11941 tcattgacct cagtatcgcg ggcaaagtga acatacaggt ggacaacaat accatcatca
    12001 cgttggggac tcccctgaat ccgcaacata acgagttcat gatggtcgcc gaatggtgcc
    12061 aatgggctat ggagcgggaa ggtttgcaag aaaacctgca aaaaaaactg cacgacgtcc
    12121 tggaagaaaa ccggcaaaac aaacagtcag aaattcccca agaagacctg aaagaaagac
    12181 tgaaagaaat caaggaagat atcctggaag aaaaccagtc agcaagccag attgaagacc
    12241 gggcagaagc attgcgcaga atgaaggaat gtctcataac aagacagagt atgctcgatc
    12301 ttagcaacct tggactgact tcactccctg aaaatttgcc tccacatctg attgaattta
    12361 actgcagtag aaacgtgttg accgccttac cggaggtaat gccaaagggg ctgagagtgc
    12421 ttgaatgtat ggagaacttt ttgatcttgt taccgaaggt gcagcccccg aaactgatgg
    12481 tactgaagtg ctatgaaaac tatattatct ggctgcctga gctgtcgact aacctgagag
    12541 tgattaactg ttctgaaaac ttcttgcaat ttttaccgcc gtcgatgccc cagtacctgt
    12601 ataaactgtc ctgtgctggc aacaatatta actcaatacc tgatgagatg ctggagaact
    12661 tgactcgcct gaaagtattt gactgttcta gtaacgattt gatctcttca ccacggctgc
    12721 cgcccaaact aataatttac tactgtggag aaaacaagtt taaaacggta caggtgccgc
    12781 agccccagag cctgaaggtg tttgactgta atggtaaccc gtgggacaaa gacaatttac
    12841 cgacgctgct caaagccgtc gagggcctga aaaaacagca gggcctgaag gaccttttgg
    12901 gctttttgca caaggagggc cagatgaagc tggagaagct ggagaagctg gaggacctgg
    12961 atgaccttat ggatctggag ttcctggatg acccggaact cctggagcgc gtgaaggtac
    13021 aggaggacct ggaactcctg aatcagcagt tgggcctgtt gagtctggaa agacaacagg
    13081 actcgcagcc tgttaatcaa caatctgaac atgaacccga acctgcatca agggtgaagc
    13141 gtgatttatc tgaagtcgag ttcgagtcaa caatgaaacg taagcgtttt atgtagaacg
    13201 cctgtgcggt gagttcaggt tccggggaaa tagtgtccat cattttaatg ggcactatca
    13261 tgggcaatgg ctctgtcaca ttaacaacta cagttgctga ccagccggat ggatgatttt
    13321 ttttgcaacc agaagacgtt gcctccgatc cggcttacaa caagttacac cgtcacaacc
    13381 gggacacgta acgccagcga gcacatcaac tcatagccca ccgttccggc agcggcggcg
    13441 acatcatcaa ttttgatctc cttgccccac agctcaaccg gcgtaccaat acccgcctgc
    13501 gggcaaggcg ttaaatcgac cgccagcata tccatcgaaa ctgtccctac tgtcatggtg
    13561 cgcacgccgt caactaaaac aggggtgccg gtaggcgcat ggcgcggata accgtcggcg
    13621 tatcctgcgg cgacaatgcc gattcgctgc tcatcgcgcg cagtataacg accgccgtag
    13681 cccacacgct cgcccgcttt tagcgtctgg acaccaataa tctcactgct tagcgtcatc
    13741 accggacgta atccggtatt ggcgatatca cgccactgac cggacggcga agcgccatac
    13801 aaaataatgc caggccgaac ccagtcaaaa tgcgcttccg ggtgccacag agtcgccgcc
    13861 gaattggaca acgaacgccg acactccagc ccttccgccg cctgctcaat acgcgccatc
    13921 gcgctggaaa ttccatcagg atgttccgcc tcggcaaaat gcgacatcag ggtcatttcg
    13981 ccaacattcg ccatcgcccg caactgctgc cagacggtaa gcacgcgatc gggctggaag
    14041 cccaaccgat tcatcccact gttcacttta agataaatat ccaacggtgc ttttagccgc
    14101 gcattttgca gtgctttgag ctgccagttg ctgtgtacgc aggtggtcag gcggtgctgg
    14161 tcataaattt ccagatcctg agcatggaaa aatccttcca gcatcaggat cggtcctttc
    14221 cagccgcgct cacgtaacgt tattgcctct tccaggttaa gcaatgcaaa gccatcggtg
    14281 gccccgagcg cgctccagat acgctcaata ccgtgcccgt aagcgttcgc tttcaccacc
    14341 gaccagacgc gcgcgtgcgg cgcggcctgg cggacaatgg acagattctg ttttaatgcc
    14401 tgcagatcga ggctggcctg tatcggacgg gtcatctcgt ttccttagct gtgtgcgcca
    14461 tgtaaatggc ccggacgtga tggcgtaaat ccacggctgt agcgcgctac gcttagatcc
    14521 tcatatggga tcgctggcgt gcgaccagag agcagatcgc ttaacaactg accggaaccg
    14581 caagccatcg tccagccgag cgtgccgtga ccggtattca gccacagatt tttaaagcgt
    14641 gtacgcccga caaccggcgt gccgtctggc gtcatcgggc gcagaccagt ccagaaagtc
    14701 gcctgctcga catgaccgcc gcgtggatag agatcgcgaa ccaccatctc cagcgtttca
    14761 cgacgcggtt gcaacagctc ggtgttaaaa ccaacaattt ccgccattcc gccaacacga
    14821 atgcggttat cgaaacgggt aatggcgatt ttgtaggttt catcaagaat ggtggatacc
    14881 ggcgcaccat cttcctgcgc aattggaatg gtcagcgagt agcctttcag cggatacacc
    14941 ggaatatcaa caatgccttt gagcatcgcc gtcgagtaag aaccaaacgc catcacatac
    15001 gcatcggcct taatcacttc atcaccacac ttcacgccgt agatttgctc gccgtcgcaa
    15061 agcagttggt caacgggcgt attaaagcgg aatgtaaccc ccgcctgctc tgccatccgc
    15121 gccagattct gggtaaatag ctgacagtct ccggtttcat cattgggtaa ctgcaggccg
    15181 cccgtcagtt tgtgcgccac ttctgccagc gcgggctcca cttccgccag gcggcaggat
    15241 tccagcagct gatacggtac gccggcatct tccagcacgg cgatatcgcg ggtcgcattt
    15301 tcatactgtt gttcggtacg gaacagttgc agcgtcccac cctgacgccc ttcatactga
    15361 atattggttt cggcgcgcaa tgctttcagg caatcacggc tgtattccgc cagacacacc
    15421 atccgccctt tgttttccat gtagtggctg gtgtcgcagt tacgtaacat ttgccacatc
    15481 catttcaact ggaattgtgt accgtcgaga cgaaccgcca gcggcgcatg gcgctggaac
    15541 atccatttga tcgcttttaa aggcacacct ggtgccgccc acggtgcagc atatccgggg
    15601 gagatttgcc cggcattcgc ggcactggtt tccagggcgg cccccggctc ccgatcaatg
    15661 acggtgacct catgtcctgc ctgatttaag taccaggcgc tggcaacgcc taccacacca
    15721 cttcccagta tgacaactcg catagccact ccgataacgg tagatacagt aaaagaataa
    15781 taatctaatt acatcttgat aactcagttg aaaatattat tcaacatacg gcttttttat
    15841 ggtgagctgg ctcacatctc cctgactctg cggcaatatg gcgtctttag tatctcctgc
    15901 tgaacgccct ttttatccag cataaaattc cgttcagaag cggattagtg gcactctgat
    15961 aataagaaat cgcaaagaaa aaaattctcc ggcgacgcgg tttttaacag gtgttctatg
    16021 cttgaaatga ggtgctccac acagagagcg ccgacaacaa tgaggtgcgc gtatggcgac
    16081 gatcgattct atgaataagg acaccacacg tttgagcgat ggacccgact ggacgttcga
    16141 cctgctggat gtttatctgg cagagataga ccgggtggcg aaattctacc ggctggatac
    16201 ctacccgcac cagattgaag tgataacctc agaacagatg atggatgcct actccagcgt
    16261 cggcatgcca attaactatc cgcactggtc attcggtaaa aagtttatcg agactgaacg
    16321 gctgtataag cacggtcagc aaggactggc ctatgaaatc gtcattaact ctaacccgtg
    16381 tatcgcttac ctgatggaag agaacaccat taccatgcaa gcgctggtga tggctcatgc
    16441 ctgctatggg cataactctt tcttcaaaaa caattactta ttccgtagct ggaccgacgc
    16501 cagttcgatt gtcgattatc tgatttttgc ccgtaaatat attaccgagt gcgaagaacg
    16561 ttatggcgtt gatgaagtag aacggcttct ggactcgtgc cacgcgctga tgaactacgg
    16621 cgtggaccgg tacaaacgtc cgcaaaaaat ctcgctgcaa gaagagaaag cccggcagaa
    16681 aagtcgcgaa gagtatctgc aaagtcaggt caatatgctc tggcgtaccc tgccgaagcg
    16741 cgaggaagag aaaacggttg ctgaagcgcg ccgttatccg tccgaaccac aagaaaacct
    16801 gctctatttt atggagaaaa atgcgccact gctggaatca tggcagcgtg aaatcctgcg
    16861 tattgtgcgt aaggtgagcc agtattttta tccgcaaaaa cagactcagg tgatgaacga
    16921 aggctgggcc accttctggc actacaccat ccttaaccat ctgtatgatg aagggaaagt
    16981 aacggaacgt tttatgctgg agtttttgca cagccacacc aatgtggtct tccagccccc
    17041 ctataacagc ccgtggtaca gcggcatcaa cccgtatgcc ctcgggttcg ccatgttcca
    17101 ggatattaaa cggatttgtc agtcgccaac ggaagaagac aaatactggt tcccggatat
    17161 cgccggttcc gactggctgg aaacgctgca tttcgcgatg cgtgatttca aagatgagag
    17221 ttttatcagc cagttcctgt caccgaaagt gatgcgtgat ttccgcttct tcaccgtgct
    17281 ggatgacgat cggcataatt atctggagat ttccgctatt cataatgaag aaggttatcg
    17341 ggagatccgt aaccggctat cgtcgcaata taacttaagt aatctggagc cgaatattca
    17401 gatctggaac gtggatttgc gcggcgaccg ttcgctgacg ctgcgttaca ttccacataa
    17461 tcgcgcaccg ctggatcggg ggcgcaaaga agtactgaag catgtgcatc gcctgtgggg
    17521 atttgatgtg atgctggaac agcaaaacga agacggcagc gtcgagttgc tggaacgttg
    17581 cccgccgaga atgggaaatc tgtaaaaaca acaaaaaacc cctcgtttga ggggtttgtt
    17641 ttttaaacgg aagggattat cgcccctgaa tggctaaatc acccggcaga tttttctgca
    17701 tccggtgcca aatctcgcca ctctcatgcc catagcgacg cactgtttcg tacacctgat
    17761 cgtgcgcgcc ttcactgcac aacgccgaca gtttgtggta gaagcccagc gccagactgc
    17821 gcgcttccgg attggcgaaa tagtgacgac caatgcgcgt atacagccct ttcatcccgt
    17881 taaggatcag accgtaaatc gggttgccgg aagcaaacgc caggccgcgg aaaatgttgt
    17941 aatccagctc ggcaaaggca tcggcgtgat cggccacttc attagcggta gccagcactt
    18001 cctgcgcttt atcgggatgc tgacgaaacg cggtgcgaat aaaaatagtt gaaatattgg
    18061 tacgcaccga cagcaaatta tcaataagct gcggcacact ttcgtgatcc agtcgcgcca
    18121 gtgtttcaag gatatttaaa ccagaggttt cccagaaatt attcaccttc gtcggcttgc
    18181 catgttgaat ggtcaaccag ccatctcgtg ccagacgctg taacacttca cgtaacgtag
    18241 tacgcgttac gccaattaat tctgaaagtt cacgttctgc gggcaaaata gtcccgggag
    18301 ggaagcggtt attccagata ctttcaataa tgtactcttc cgcgaaaccc gccgggcttt
    18361 gcgccttaat gaccatagtg agatttccat aacacagcaa aacaaagttg gactcatcat
    18421 accagagggc taactacgct gatagcagac aaaaagaaga aaaaagggat caaggcttca
    18481 ttttccgcat ctctttcccc ttgactatca agacttgcct cactcctgcc tgaccgttaa
    18541 gctttgcgtt taaacataaa aacatcattg attatttgta aggtaaggga aaccatcatg
    18601 gagatctcct ggggccgcgc gctatggcgc aattttttgg gccagtcccc cgactggtac
    18661 aaactcgccc tcatcatttt cttaatcgta aatccgttaa ttttcctcat tagccctttc
    18721 gtcgctggct ggttgctggt agcggaattt attttcactc tggcgatggc cctgaaatgc
    18781 tacccgctgc tccccggtgg cctgttggct atcgaagcgg tattcatcgg catgaccagc
    18841 gcggaacacg tccgtgaaga ggtggcggca aatcttgaag tcttgctgtt attgatgttt
    18901 atggtggcgg gtatctattt tatgaaacag ctgttgctgt tcatatttac ccgtttgctg
    18961 ttaagcattc gctccaaaat gctgctgtcg ctctcttttt gcgtggcggc agcgttcctc
    19021 tccgcgttcc tcgatgcctt aaccgtcgtg gcggtggtga tcagcgttgc agtcggtttt
    19081 tatggtattt atcatcgcgt agcctcttcc cgtaccgaag acaccgacct gcaagacgat
    19141 agtcatatcg acaagcatta caaagtggtt ctggaacagt tccgtggctt tctgcgtagc
    19201 ctgatgatgc atgccggtgt cggcaccgca ttaggcggcg tgatgaccat ggtgggcgaa
    19261 ccacagaacc tgatcatcgc taaagcggct ggctggcatt ttggcgattt cttcctgcgc
    19321 atgtcgccgg tgaccgttcc ggttctgatt tgtggcctgt taacctgcct gctggtagag
    19381 aagctgcgtt ggtttggcta cggtgaaacg ctgccggaga aagtccgcga agtcctgcag
    19441 cagtttgacg atcaaagccg ccaccagcgc acccgtcagg ataaaatccg tctgattgtc
    19501 caagcgatta ttggcgtctg gctggtgact gcgctggcgt tgcatctggc ggaagttggc
    19561 ttgattggtt tgtcagtcat tattctggca acatcattga ccggtgtcac cgatgaacat
    19621 gctatcggta aagccttcac cgaatctctg ccattcaccg cactgttgac ggtgtttttc
    19681 tcggtagtcg cggtgattat cgaccaacaa ctgttttcgc cgattattca gtttgtgttg
    19741 caggcatcgg aacatgctca gctgtcgttg ttctatattt tcaacggact gctgtcatcc
    19801 atttcggata acgtcttcgt ggggacgatt tatatcaacg aagcgaaagc ggcaatggaa
    19861 agtggcgcta tcacgttgaa gcaatacgag ctgctggcgg tcgccattaa taccggtacc
    19921 aatctgccct ctgtcgctac gccgaacggt caggctgcgt tcctgttcct gctgacctct
    19981 gcactcgcgc cattgattcg cctttcttat ggccgcatgg tgtggatggc cctgccttac
    20041 accctcgtcc tgacactcgt cggactgctc tgcgtcgagt ttacgcttgc ccctgtaacc
    20101 gaatggttta tgcaaatggg ctggatagca acgctttgat aacaacttac cgggcatata
    20161 aatgcccggt ttgccttttc gcccgataat tgtccaattg cgtcctattt ttgctgcctc
    20221 ctggtggcgc agcgaatgaa ttggtttaaa ctgcgcactc tatgcatatt gcagggaaat
    20281 gattatgttg cgatttttga accaatgttc acaaggccgg ggcgcgtggc tgttgatggc
    20341 gtttactgct ctggcactgg aactgacggc gctgtggttc cagcatgtga tgttactgaa
    20401 accttgcgtg ctctgtattt atgaacgctg cgcattattc ggcgttctgg gtgctgcgct
    20461 gattggcgcg atcgccccaa aaacgccact gcgttatgta gcgatggtta tctggttgta
    20521 tagtgcgttc cgcggtgtgc agttaactta cgagcacacc atgcttcagc tctatccttc
    20581 gccgtttgcg acctgtgatt ttatggttcg tttcccggaa tggctgccgc tggataaatg
    20641 ggtgccgcaa gtgtttgtcg cctctggcga ttgcgccgag cgtcagtggg attttttagg
    20701 tctggaaatg ccgcagtggc tgctcggtat ttttatcgct tacctgattg tcgcagtgct
    20761 ggtggtgatt tcccagccgt ttaaagcgaa aaaacgtgat ctgttcggtc gctaatccat
    20821 tcggcgctcc tgcgggagcg cttttttcct gccgctatat ttatttgacc cgcagtaaat
    20881 cagaacttcg cgttgtataa cgtggtgaaa gcatggctcg cttcatctgc cattgttgct
    20941 ggatcccctg cccggcaaaa tagagtgttc ctctgccctc tttagcattc agtgtatcca
    21001 ttaccgtcat caattgctca ctcccggggc gcggtgcgtt gtcatcgaat aaattgagct
    21061 gcgcgactcc ctgactgaag aaatccccca gcatcacgcc cgctttttgg tagcgatggc
    21121 ccgcttgcca gatggcatcc agagatcgtg tagcggcgtt aatgatatcc ctgctgtcct
    21181 gagtgggcgt cagcagtttt accgacgcgc tattgccgta ataaggttca ttgagcgcaa
    21241 atggtgacgt cttaataaat gtggagataa accgacaata ttgatgctca ctgcgaagtt
    21301 tttccgccgc ccgggcagcg taactacaaa tggcctgccg catcgacgta taatccgtga
    21361 tgcgttcacc aaacgagcgg gaacagataa tttcctgctt tgtcggtgca aattcttcca
    21421 gttgcaaaca gggttcaccg cgcagttcac gcaccgttct ttcgagcacg acattaaaat
    21481 gtttacggat aaaccggata tctgtatccg ccaaatcgag aacggttttg atccccatcg
    21541 cgtccagttt tttgctgatc cgccgtccaa tcccccagac gtcatccacg gggagagcag
    21601 acattaattt acgctggcgt tccagatttg ataaatccac caccccaccc gtctgccgct
    21661 gccatttttt tgccgcatga ttggcaagct tagccagcgt tttggtctgg gcgatcccca
    21721 caccaacagt aagatgggta cgttgtagca ccgttgcgcg aatttctctg ccaaagtcag
    21781 tcagatcgcg acaattacgc acacctgtca gatcgcagaa tgcctcatca atactgtaaa
    21841 tctcgacgcg gggcgatagt tcttccagcg tcgacatcac ccgattgctc atgtctgcgt
    21901 aaagctcata attgctgcta aagcaaacca cgccacagcg acgaaacaga tctttttgtt
    21961 tgaaccaggg atcgcccatt ttaacgccaa gcgcctttgc ctcagcgttt cgggcgataa
    22021 cgcaaccgtc attattcgat agcacaacca ccggtttacc ccataaatca gggcgaaaca
    22081 ccgtctcaca gctggcataa aacgcgttta catcacagag ggcaaacatc agcgcatcgc
    22141 cttaacgacg tggatcacca caccaaagac atccagcgta tcttcactac tgatggtaat
    22201 gggcgagtac gcgctgttca tgggaataag ctgtaccgtc gggcgtagtt gcaatttttt
    22261 caccgtaaac tcgccgtcaa cagcagcgat gacaatatca ccatggctgg cggtaatagc
    22321 gctatcgaca atcagtaaat caccgtcact aattccacca tcaatcatag aatcaccact
    22381 tgctttgacg aagtaagtcg cgctgggatg ctggatcaac agttgattca gatcgatgcg
    22441 ctgttcaacg taatctgcgg ccggtgaagg aaagccacac tgaacaagat cgctaaatag
    22501 cggaaaagtc acaatttcgc ggagatccgc aggcttgata aacaacataa taatctgcct
    22561 gaagttatac tgtttttata tacagtagtc tgttcttgcc agcagatcaa tactgattca
    22621 ggctatcaat atttgtcgct gcatagactg ctgatttttc gttctcttat cctgtgctca
    22681 cgcggccttc tggcgacgac gctcatccag cagaaatgaa aaatatcacc cggctaaaaa
    22741 atagaataga agcatt
//
LOCUS       DAPGRN010000075        22556 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPGRN010000075 DAPGRN010000000
VERSION     DAPGRN010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.075"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..531
                     /locus_tag="RHW33_003058"
     CDS             <1..531
                     /locus_tag="RHW33_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1646538.1"
                     /translation="NWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLK
                     VSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCAYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(571..1785)
                     /gene="ydcO"
                     /locus_tag="RHW33_003059"
     CDS             complement(571..1785)
                     /gene="ydcO"
                     /locus_tag="RHW33_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV1646539.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGASTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPSYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1838..2374
                     /gene="sutR"
                     /locus_tag="RHW33_003060"
     CDS             1838..2374
                     /gene="sutR"
                     /locus_tag="RHW33_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV1646540.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2447..4408
                     /gene="rlhA"
                     /locus_tag="RHW33_003061"
     CDS             2447..4408
                     /gene="rlhA"
                     /locus_tag="RHW33_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV1646541.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTDPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4500..4730)
                     /locus_tag="RHW33_003062"
     CDS             complement(4500..4730)
                     /locus_tag="RHW33_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV1646542.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4952..5128
                     /gene="hicA"
                     /locus_tag="RHW33_003063"
     CDS             4952..5128
                     /gene="hicA"
                     /locus_tag="RHW33_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV1646543.1"
                     /translation="MKQSEFRRWLESQGVDVANVSNHLKLGFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5174..5590
                     /gene="hicB"
                     /locus_tag="RHW33_003064"
     CDS             5174..5590
                     /gene="hicB"
                     /locus_tag="RHW33_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV1646544.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5669..7075
                     /locus_tag="RHW33_003065"
     CDS             5669..7075
                     /locus_tag="RHW33_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV1646545.1"
                     /translation="MKKYQQLAEQLREQIVTGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7320..8465
                     /locus_tag="RHW33_003066"
     CDS             7320..8465
                     /locus_tag="RHW33_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV1646546.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALITNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLQDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8483..9496
                     /locus_tag="RHW33_003067"
     CDS             8483..9496
                     /locus_tag="RHW33_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1646547.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAMQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9497..10438
                     /locus_tag="RHW33_003068"
     CDS             9497..10438
                     /locus_tag="RHW33_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646548.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPIAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10428..11222
                     /locus_tag="RHW33_003069"
     CDS             10428..11222
                     /locus_tag="RHW33_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646549.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11244..12668
                     /gene="patD"
                     /locus_tag="RHW33_003070"
     CDS             11244..12668
                     /gene="patD"
                     /locus_tag="RHW33_003070"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV1646550.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(12765..12860)
                     /gene="yncL"
                     /locus_tag="RHW33_003071"
     CDS             complement(12765..12860)
                     /gene="yncL"
                     /locus_tag="RHW33_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV1646551.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            13055..13228
                     /gene="ortT"
                     /locus_tag="RHW33_003072"
     CDS             13055..13228
                     /gene="ortT"
                     /locus_tag="RHW33_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV1646552.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            13314..13547
                     /locus_tag="RHW33_003073"
     CDS             13314..13547
                     /locus_tag="RHW33_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV1646553.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(13548..13997)
                     /locus_tag="RHW33_003074"
     CDS             complement(13548..13997)
                     /locus_tag="RHW33_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1646554.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(13994..14512)
                     /gene="mddA"
                     /locus_tag="RHW33_003075"
     CDS             complement(13994..14512)
                     /gene="mddA"
                     /locus_tag="RHW33_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV1646555.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVITAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            14693..15730
                     /gene="curA"
                     /locus_tag="RHW33_003076"
     CDS             14693..15730
                     /gene="curA"
                     /locus_tag="RHW33_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV1646556.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVKSNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMEQWVKEDKIHYREEITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            15928..16593
                     /gene="mcbR"
                     /locus_tag="RHW33_003077"
     CDS             15928..16593
                     /gene="mcbR"
                     /locus_tag="RHW33_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV1646557.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(16629..18731)
                     /locus_tag="RHW33_003078"
     CDS             complement(16629..18731)
                     /locus_tag="RHW33_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDV1646558.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEANSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGSVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            18973..20034
                     /locus_tag="RHW33_003079"
     CDS             18973..20034
                     /locus_tag="RHW33_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV1646559.1"
                     /translation="MHLRHLFSLRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(20149..21648)
                     /gene="ansP"
                     /locus_tag="RHW33_003080"
     CDS             complement(20149..21648)
                     /gene="ansP"
                     /locus_tag="RHW33_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV1646560.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTINM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            21915..22511
                     /locus_tag="RHW33_003081"
     CDS             21915..22511
                     /locus_tag="RHW33_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV1646561.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTPEPYAFGEQLTLVDC
                     YLCTMRTWGLGMNGFRIMPRISVRLLMLYVSYRNYKKY"
BASE COUNT         5736 a         5549 c         5834 g         5437 t
ORIGIN      
        1 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
       61 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
      121 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
      181 cgcaatgtcc gggcaaaatc aggtttaaac cgttcgatac tggatcaggg ctggtatgaa
      241 atgcgccgcc agcttgagta caagcagctc tggcgtggcg gtcaggtgct tgctgttccg
      301 ccagcgtata caagccagcg ttgcgcgtac tgtggtcata cagcgaaaga gaaccgcctg
      361 tcacaaagta aattcagatg ccaggtatgt ggatatacag cgaacgccga tgtaaatggc
      421 gctcgtaaca ttttagcggc ggggcacgcc gttcttgcct gtggagagat ggtgcagtca
      481 ggccgcccgt tgaagcagga acccaccgaa atgattcagg cgacagcctg aacgtagcag
      541 ggatccacgt ccttcagggc gtggaggatg tcaatatcgg tttctgtcag cgattaaatt
      601 caacaccacg taacaaacgc ctccggcaat taatccccag aacgcagaac caatcccgac
      661 cagcgtcaat ccacttgccg ttaccagaaa tgccaccacc gccgcgtcgc gctcacgctc
      721 attatgcagc gcctgataca aactgccgcc aatggtactt aacagcgcca gacctgccag
      781 catctggatc caacttacgg gcagggcagc catcatcccg gtaatggcac taccaaacag
      841 acctgcgagc aaatagaaaa tgcctgcaac ggcagctgcc agccaacgtt gatctttatc
      901 cggatgcgct tccgggcttt ggcaaatagc cgcggtgatt gccgcaatac cgactgaata
      961 aacgccgaaa ggggaaaaaa acagtgccag caatccagta aatacaatta atggcgaaac
     1021 aggagccgaa tatccggcgg ctttcattgc tgcgataccc ggtgcgtttt gcgatgccat
     1081 cgtcaccaga aaaaggggga gtgcaacgct caggctgtga gcaaacgaaa aatcaggggt
     1141 aatataagag gggagaacgg gtttaaaaac aacatcagtt gtgacaacgt caccttgcgc
     1201 gataacgatc acgatcccaa taatcatcgc ggcaattacc gcatagcgcg gcgcaacggc
     1261 cttggttgcc agccatacca gcaacatact tccacacaac gtaaattgac cgtccagact
     1321 ggcaaacgcc tgtaaaccaa agcgtaataa aatcccggca agcattgccg ccgcaagcga
     1381 gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga ggactattaa
     1441 cgcgttggtg acaataaaaa cgccgatggc ttcgttaagc gttagtccct gcaatccggt
     1501 gaccaacaaa gccgcgccag gcgttgacca tgcggtgaga acaggtacgc gataccataa
     1561 tgtcagagtc agcgtactga cgcccattgc cagccccagc gccgtcatcc agccagagat
     1621 ttgtgcagtg gaggctccgg cgacaatcgc tgcttgccag attattgccg ctgaactggc
     1681 gtagccaatt aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa tagaaaacag
     1741 acgcatagtc acctcgtgcg ttatagcgta caggcaccgt agcatttgtc cgttataacg
     1801 cacaagtgat aaacttccgt tttgccggag gagtcgcatg gaaaatctcg ctcgcttttt
     1861 atccaccaca cttaaacaat tacgtcagca gcgcggctgg agtctttcgc gactggcaga
     1921 agcgacgggc gtttctaaag caatgttagg acagattgag cgtaatgagt ccagcccgac
     1981 ggtcgcgaca ttatggaaaa ttgccactgg gttgaacgtg cccttttcga catttatttc
     2041 tccgccacag tccgctacgc cttcagttta tgatccacaa cagcaggcga tggtgatcac
     2101 ttcgctgttt ccttatgatc cacaactctg ctttgaacac ttctcgattc agatggcacc
     2161 cggtgcaata agcgaatcga cgccccatga gaagggggta atcgaacatg tcgtcgtcat
     2221 tgatggacaa cttgatctgt gcgttgatgg ggaatggcag agccttaatt gtggggaagg
     2281 cgttcgattt gctgcagacg tcacgcatgt ctatcgtaat ggtggggagc aaaccgtaca
     2341 ttttcattcc ctcatccatt acccgcgcag ttaagcggga aaactatttc gcaacgtcgc
     2401 gcttgtggct aaaatagccg ccatttttca gctactggat aagaatgtga ccgtatcttc
     2461 tcatcgactt gaactgttaa gcccggcacg cgatgccgcc attgcccgcg aagctatttt
     2521 gcacggtgct gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc ataatgccag
     2581 taatagcctg aaagatattg ccgagctggt gccgtttgcc catcgttatg gtgcaaaaat
     2641 tttcgtcacg cttaacacca ttttgcatga tgatgagctg gaacccgcgc aacggctgat
     2701 tactgacctc taccagaccg gtgtcgatgc gctgattgtt caggatatgg ggattctgga
     2761 acttgatatt ccgccgattg aactgcacgc cagtacgcag tgcgacattc gtacagtaga
     2821 aaaagcgaag ttcctctctg atgttggctt cacgcagatt gtgctggcgc gagagctgaa
     2881 tcttgatcag atccgcgcga ttcaccaggc cacggacgcg accattgaat tctttattca
     2941 tggcgcactg tgcgtggcct attcgggtca gtgctacatt tctcatgcgc aaacagggcg
     3001 tagcgccaac cgtggcgatt gctcgcaggc gtgccgtttg ccatacacat tgaaagacga
     3061 tcaggggcgg gtggtttcct atgaaaaaca tctgctgtcg atgaaagata acgatcagac
     3121 tgccaacctc ggcgcgctga ttgatgctgg cgtacgctcc ttcaagattg aagggcgtta
     3181 caaagatatg agctacgtga agaatatcac cgcccattat cgccagatgc tggatgccat
     3241 tattgaagaa cgtggtgatc tgacgcgcgc ttcatcaggt cgtactgaac atttctttgt
     3301 tccatcgacg gaaaagactt tccatcgtgg tagcacagat tattttgtga atgcccgtaa
     3361 aggcgatatt ggcgcgttcg attcgccgaa atttatcggc ctgccggtgg gtgaagtatt
     3421 gaaagtggcg aaagatcatc tcgatgttgc cgttaccgat ccactggcaa atggcgatgg
     3481 cctgaacgtg ttgattaaac gtgaagtcgt cggttttcgt gccaatacgg tcgagaaaac
     3541 cggagaaaat cagtaccgcg tctggcccaa tgaaatgcca gcagatttgc acaaaattcg
     3601 cccgcatcac ccactaaacc gtaatcttga tcataactgg cagcaggcac tgacaaaaac
     3661 ctccagcgaa cgtcgggtgg cggtagacat tgaactgggc ggttggcagg aacaattgat
     3721 tctgaccctc accagtgaag agggggtcag catcacgcat acgctggatg ggcagttcga
     3781 cgaagccaat aacgccgaaa aagcaatgaa caatctgaag gatggtctgg caaaactggg
     3841 gcaaaccatc tattacgccc gcgatgtgca aattaatttg ccgggggcac tgtttgtacc
     3901 aaacagtctg ttaaaccagt tccgccgtga agctgctgac atgctggatg ctgcgcgtct
     3961 tgccagttac cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg tttatccgca
     4021 aacgcatctg agtttcctcg cgaacgttta caaccagaaa gcgcgtgaat tttatcatcg
     4081 ctatggtgtg cagctgattg acgcggcgta tgaggcacat gaagagaagg gcgaagtccc
     4141 ggtgatgatc accaagcatt gtctgcgctt tgcctttaat ttgtgcccga aacaggcgaa
     4201 aggcaatatc aaaagctgga aggctacgcc aatgcaactg gttaacggcg atgaagtatt
     4261 aacgctaaag tttgattgcc gcccatgcga gatgcacgtc attggcaaaa tcaaaaatca
     4321 catactgaaa atgcctttac cgggaagcgt agtggcatcc gtaagtccgg atgagctgct
     4381 gaaaacattg ccgaagcgaa aagggtaaaa cgccagtttt ctggttactc acaacttatt
     4441 gaatctgcat gatattgcct gccgggtaag gcgttacgcc gcatccggca tcaaacgact
     4501 caacgttgtc ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc gcgcaaacct
     4561 tccgccgact cttccgccac agcacgcaat tcatcgctgt ccgcttcatg acgcagctga
     4621 tgatccacat tctttaccca cacaaattca tgtcctttgt gccctgccat gagttgtcct
     4681 gagaacagcg cacacgttaa taagacaacc gataacgcct tcgtaaacat cctgccacct
     4741 ttttcttacc ttttgccgct atgatgccga tcgtttcttg aggttattat tcagttttgc
     4801 aaattagcgc aaagaaattc tggaatcttc cttcctgatt ttgcattgca ttctgccgtt
     4861 gcggcgattt agtgctattt tcgagcaaat tacacacgga ggtaaacact aatgtttact
     4921 ttgttgatat actcagcggc agggaggcga tgtgaaacaa agcgagttca gacgttggct
     4981 cgaatctcag ggcgtcgatg tagcgaatgt cagcaaccat ttgaaacttg ggtttcatgg
     5041 gaggcgcagt gtcatgccgc gtcacccctg cgatgagatt aaagaaccat tgcgtaaagc
     5101 aatcctgaaa caactcggtt tgagttaatc gccaattaaa aaggttaatg acatgcgaga
     5161 gacagtcgaa attatgcgtt atcccgtcac tcttacaccc gcgccggaag gcggttatat
     5221 ggtttctttt gtggatatcc ctgaagcgtt gacccagggc gaaactgtcg ctgaagcgat
     5281 ggaagcggca aaagatgctt tactgaccgc atttgatttt tattttgaag ataacgagct
     5341 tatcccgtta ccttcgccat taaatagtca cgatcatttt attgaagtac ctttgagcgt
     5401 cgcctctaag gtattgctgt taaatgcttt tttacagtca gaaatcactc agcaagagtt
     5461 agccaggcga attggcaaac ctaaacagga aattactcgc ttatttaact tgcaccatgc
     5521 gacaaaaatc gacgccgtcc agctcgcggc taaggcgctt ggcaaagagt tatcactggt
     5581 gatggtttga taacagttaa tgaaaagttg tcatttttaa caactgatat agactgccga
     5641 atcatctgca cataattacg attcgataat gaaaaaatac cagcagcttg cagaacaatt
     5701 acgcgagcag attgtgacgg gtatctggca acccggcgat cgtttgcctt cgttgcgtga
     5761 ccaggtggcg ctttctggca tgagctttat gactgtcagc catgcctatc agttgctcga
     5821 aagtcaggga tatattatcg cacgaccgca gtcgggttat tacgttgcgc cacaggcaat
     5881 aaaaatgccg aaagcgccag tcattccagt cactcgagat gaagcagtcg atatcaacac
     5941 ttatattttt gatatgttgc aggccagtcg cgatccgtcg gtcgttccgt ttgcctcggc
     6001 ctttcccgac ccgcgacttt tccccctcca acaactaaac cgctcgctgg cgcaggtaag
     6061 caaaaccgcc acggcgatga gcgtgattga aaacttgccg ccaggaaacg cagaactgcg
     6121 tcaggctatt gcccgtcgct atgccttaca gggcatcacc atttctcctg atgaaattgt
     6181 catcactgcc ggggcgttag aagcattaaa cctcagtttg caagcggtaa ctgaaccggg
     6241 cgattgggtg atagtagaga atccttgttt ctacggtgcg ttgcaggcgc tggagcggct
     6301 acggctgaag gcgttatcgg tggcgacgga tgttaaagaa gggatcgatc ttcaggcgct
     6361 ggaactggcg ttgcaggagt atccggtaaa agcgtgctgg ctgatgacta atagccagaa
     6421 tccactcgga tttaccttaa cgccgcaaaa aaaagcacaa ctggtggcgt tgctcaatca
     6481 gtacaacgta acgctgattg aagatgacgt ttacagcgaa ctttattttg gacgggaaaa
     6541 accgctgcct gcgaaagcgt gggatcgcca cgatggcgtt ttgcattgct cttcgttttc
     6601 gaaatgtctg gtgcctggtt ttcgtattgg ttgggtcgcc gccggaaaac atgcacgtaa
     6661 aattcaacgc ttgcagctga tgagtacgct ttccaccagc tcaccgatgc agcttgcgct
     6721 ggtggattac ctttccacgc gccgatacga cgcccatctt cgtcgcctgc gtcgccagct
     6781 tgcggaacgt aaacaacgtg cctggcaggc actgctgcgt tatctgcctg cggaagtgaa
     6841 aattcatcat aatgacagcg gttattttct ctggctggag ctccccgagc cgttagatgc
     6901 cggcgaattg agcctggcgg cactgacgca tcatatcagt attgcgccgg gtaaaatgtt
     6961 ttctaccggt gaaaactggt cacgtttttt tcgttttaat accgcgtggc agtggggaga
     7021 acgtgaagaa caggcggtaa aacaattagg caaacttatt caagaacggc tgtaatagcg
     7081 tttaatttaa ttcctcttag attgggtaat atgaatttcg aatagcagtc atatttccta
     7141 actccttgtc tatactccag aagataacct tacagacggc ataatgcgcg gtagctcaca
     7201 acctgattaa attttctcag gggcgaaggt gtgcctgcaa gccgccgtct atggttaaac
     7261 aaggagatat ttttacggca cggcggctga acaattaatt acgacaggag taagacctta
     7321 tgagcaagac atttgcccgc agcagcctgt gtgcgctcag catgacaata atgaccgctc
     7381 acgccgccga accgcctacc aatttagata aaccggaagg gcgactggat attatcgcct
     7441 ggccgggata tatcgaacgc ggacaaactg ataaacaata cgactgggta acgcaattcg
     7501 aaaaagagac aggctgcgcg gtgaatgtta aaaccgccgc gacttccgat gaaatggtca
     7561 gtctgatgac caaagggggt tacgatctgg ttacggcatc cggcgatgcc tcgctgcgtt
     7621 tgattatggg taaacgcgtg cagccgatta ataccgcctt gattaccaac tggaaaacgc
     7681 tcgatccgcg cgtggttaaa ggcgactggt ttaacgttgg cggcaaagtt tacggtacac
     7741 cttaccaatg ggggccgaac ctgctgatgt acaacactaa aaccttcccg acgccgccgg
     7801 atagctggca agtggttttt gttgagcaaa atctgcagga cggcaagagc aataaaggcc
     7861 gcgttcaggc ttatgatggc cctatctaca ttgcggacgc tgcgttgttc gttaaagcca
     7921 ctcagccgca gttgggcatc agcgatccgt atcaactcac cgaagaacag taccaggcgg
     7981 tgctgaaagt gctgcgcgct caacatagtt tgatccatcg ctactggcat gacactaccg
     8041 tgcaaatgag cgatttcaaa aacgagggtg tggttgcttc cagtgcatgg ccctatcagg
     8101 ccaacgcctt gaaagccgaa ggccagcctg ttgctaccgt tttcccgaag gagggtgtta
     8161 ccggttgggc tgataccacc atgctgcata gcgaagcgaa acatccggtt tgcgcctaca
     8221 aatggatgaa ctggtcatta acgccaaaag tgcagggcga tgtggcggcc tggtttggct
     8281 cgttaccggt agtgccgcaa gggtgtaaag ccagtccgtt attaggcgaa aaaggttgtg
     8341 aaaccaacgg ctttaactat ttcgataaaa tcgccttctg gaaaacgcct atcgcagaag
     8401 ggggcaagtt tgttccctac agtcgctgga cgcaggatta cattgccatt atgggcggtc
     8461 gctaacttcg ctggggtgtt ttatgacgta cgcagtggag tttgacaacg tctcgcggtt
     8521 gtacggtgac gtgcgcgcag tagatggcgt cagtattgcg ataaaagatg gtgagttctt
     8581 ctctatgctg gggccgtccg gctccggcaa aaccacctgc ctgcgcctga ttgctggctt
     8641 cgaacagctt tccggcgggg ctatctctat ctttggtaaa cccgccagca atctgccacc
     8701 gtgggagcgg gacgtgaata ctgtctttca ggactacgcg ctatttccgc atatgtcgat
     8761 tcttgacaat gtcgcctatg ggctgatggt caaaggcgtg aataaaaagc agcggcacgc
     8821 aatggcgcaa gaggcgctgg agaaagtggc gttggggttt gtacagcaac gtaaaccgtc
     8881 acaactttct ggtggtcagc gccagcgggt tgctatcgcc agagcattgg tgaatgaacc
     8941 gcgcgtattg ctgttggatg aaccactcgg cgcactggat ctcaaattgc gtgagcagat
     9001 gcagctggaa ctgaaaaaac tgcaacagtc tctcggtatc acttttatct tcgttaccca
     9061 cgatcagggc gaagcgttat cgatgtccga tcgtgtggcg gttttcaata atggacgcat
     9121 tgagcaggtc gattccccgc gcgatctcta tatgcgcccg cgcacgccgt ttgttgccgg
     9181 gttcgttggt acatcgaatg tttttgatgg actgatggca gagaaacttt gtggcatgac
     9241 gggaagcttc gccctgcggc cggaacatat ccgcctcaac acccctggtg aactgcaggc
     9301 caatggcacg atccaggcga tgcaatatca gggcgcggca actcgttttg aactgaaatt
     9361 gaacggcggt gaaaaactgc tggtgagtca ggccaatatg acaggcgaag aactgcctgc
     9421 cacgctcacg cccggacaac aggtgatggt ttcctggtcg cgtgatgtga tggtgccgct
     9481 ggttgaggag aggtgaatgg cgatgaacgt attgcaatca ccttcacgtc caggtctggg
     9541 taaggtgtcc ggtttcttct ggcggaatcc ggggctgggg ctgtttttgc tgctgcttgg
     9601 cccgctaatg tggtttggca ttgtctattt cggctcgctg ctaacgctgt tatggcaggg
     9661 cttttatacc tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc tggcgaacat
     9721 ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga ctatggctgt
     9781 ggcagtcact atcgccagcg ccattctggc ttttccaata gcgtggtata tggcgcgcta
     9841 taccagcggg aaaatgaaag cgttttttta tattgcggta atgttgccga tgtgggcgag
     9901 ctacattgtt aaagcctatg cctggacgtt attgctggca aaagatggcg tggctcagtg
     9961 gtttttacaa catcttgggc tggaaccact gctgacagcg ttccttacat tacctgcggt
    10021 gggcggcaat acgctgtcaa cttccgggct ggggcgcttt ctggtgtttc tctatatctg
    10081 gctgccgttc atgatcctgc ccgtgcaggc agcgcttgag cgtttgccgc cgtcattgtt
    10141 gcaggcgtca gctgatctcg gcgcacgtcc acgacaaacc tttcgctatg tggtgctgcc
    10201 gctggcgatc ccgggtattg ccgctggctc tatctttacc ttctcactca cactgggcga
    10261 ttttatcgtc ccgcagctgg ttggcccgcc aggatatttt atcggcaata tggtttattc
    10321 ccagcagggg gcgattggca atatgccgat ggcggcggca ttcaccctgg tgccgattgt
    10381 tcttatcgca ctgtacctgg cgttcgtgaa acgtctggga gcgttcgatg cactctgaac
    10441 gcgcaccgtt tttcctcaaa ctggcggcct ggggcggcgt tgttttccta cattttccca
    10501 tcctgataat cgccgcctat gcgtttaaca ctgaagatgc ggcgtttagt tttccaccgc
    10561 agggcctgac gctgcgctgg tttagcgtgg cagcacagcg tagtgatatt ctcgatgccg
    10621 tgacactgtc acttaaagta gcggcgctgg cgacattaat ggcgctggtg ttagggacgc
    10681 tggcagctgc cgcgctgtgg cgacgagact ttttcggcaa aaacgccatt tcgctgttac
    10741 tgctgctgcc cattgcgctg ccgggcattg tcactggtct ggcgttatta accgccttta
    10801 aaaccatcaa tctggagccg ggatttttca ccatcgtggt cggtcatgcg actttttgtg
    10861 tagttgtggt gtttaacaat gtcatcgccc gttttcgccg cacctcgtgg agtctggttg
    10921 aggcgtcaat ggatcttggg gccaatggct ggcaaacctt ccgctacgta gtgttgccga
    10981 atctcagttc ggcgttactg gcaggaggaa tgctggcgtt tgccttgtcg ttcgatgaaa
    11041 tcatcgttac gacctttacg gcaggtcatg aacgaacgtt accgttgtgg ttgctcaatc
    11101 agcttgggcg accgcgtgat gtaccggtaa ctaacgtggt ggcactgctg gttatgttgg
    11161 taacaacctt gccgatcctg ggggcctggt ggctaacccg cgaaggcgac aatggtcaat
    11221 aaccactgat acaggaatat gctatgcaac ataagttact gattaacgga gaactggtta
    11281 gcggcgaagg ggaaaaacag cctgtctata atccggcaac gggggacgtt ttactggaga
    11341 ttgccgaggc atccgcagag caggtagatg ctgctgtgcg cgcggcagat gcagcatttg
    11401 ccgaatgggg gcaaaccacg ccgaaagtgc gtgcggaatg tctgctgaaa ctggctgatg
    11461 taatcgaaga aaatggtcag gtttttgccg aactggagtc ccgtaattgt ggcaaaccgc
    11521 tgcatagtgc gttcaatgat gaaatcccgg cgattgtcga tgtttttcgc tttttcgcgg
    11581 gtgcggcgcg ctgtctgaat ggtctggcgg caggtgaata tcttgaaggt catacttcga
    11641 tgatccgtcg cgatccgttg ggggtcgtgg cttctatcgc accgtggaat tatccgctga
    11701 tgatggcagc gtggaaactg gctccggcgc tggcggcagg gaactgcgta gtgcttaaac
    11761 catcagaaat taccccgctg accgcgttga agttggcaga gctggcgaaa gatatcttcc
    11821 cagcaggcgt gattaatgtg ctgtttggca gaggcaaaac ggtgggcgat ccgctgaccg
    11881 gtcatcctaa agtgcggatg gtgtcgctga cgggctctat cgccaccggc gagcacatca
    11941 tcagccatac cgcgccctcc attaagcgta ctcatatgga acttggtggc aaagcgccag
    12001 tgattgtttt tgatgatgcg gatattgaag cagtggtcga aggtgtacgt acatttggct
    12061 attacaatgc cggacaggat tgtactgcgg cttgtcggat ctacgcgcaa aaaggcattt
    12121 acgatacgct ggtggaaaaa ctgggtgctg cggtggcaac gttaaaatct ggtgcgccgg
    12181 atgacgagtc tacggagctt ggacctttaa gctcgctggc gcatctcgaa cgcgtcagca
    12241 aggcagtaga agaggcgaaa gcgacagggc acatcaaagt gatcactggc ggtgaaaagc
    12301 gcaagggtaa tggctattac tatgcgccga cgctgctggc tggcgcgtta caggacgatg
    12361 ccatcgtgca aaaagaggta tttggtccag tagtgagtgt tacgctcttc gacaacgaag
    12421 aacaggtggt gaactgggcg aatgacagcc agtacggact tgcatcttcg gtatggacga
    12481 aagatgtggg cagggcgcat cgcgtcagcg cacggctgca atatggttgt acctgggtca
    12541 atacccattt catgctggta agtgaaatgc cgcacggtgg gcagaaactt tctggttacg
    12601 gcaaggatat gtcactttat gggctggagg attacaccgt cgtccgccac gtcatggtta
    12661 aacattaagg ataatattgc agatcgtaag agtatgagat gatcttgcgt actgtcagag
    12721 cactgatttc tctgacagta cgtgctgcgt gcaaaatcac tacatcaaat aaaccagcca
    12781 aatctcatag agataagagt aaacaaacca acagcagcaa agacatttat cagtactaca
    12841 gttctactgg aaacattcat ttttgccacc ttttatcatt gcccctatgt gtatagtacg
    12901 ggttaagaaa atccgcaata atactcattc tgaagatgga taagggcaag ttgctgtttg
    12961 atgttttttt ctcaaaccgg ttaaaaaact tgatgcacgt caaaaaatga cgcatatttg
    13021 cgcgcgtttt attcatctgg ctggacgccc gtacatgtct ctctatcaac acatgcttgt
    13081 tttttatgcg gttatggcag caatcgcatt tcttatcact tggtttcttt ctcacgataa
    13141 gaaacgcatc cgtttcttaa gtgcctttct ggtgggggca acatggccga tgagtttccc
    13201 ggtggcgctg ttgttttcac tgttttaaat cttactcact ggcttgacgg caccacgcag
    13261 acttatatca tttggatgaa tcgataaatt tcacaagtgg ctaaggagaa agtatgtcgc
    13321 atctggatga agtcatcgcg cgtgtggatg ccgcgattga agagagcgtc attgcccata
    13381 tgaacgaatt attaatcgcc ctgagcgatg acgcggagtt aagtcgggaa gatcgctaca
    13441 cccagcagca gcgtttgcgc accgctattg ctcatcacgg tcgcaagcat aaagaagata
    13501 tggaagcgcg ccacgaacag ttaaccaaag gcggcaccat cctgtgatta aaatgagcgt
    13561 ctggccacca accaggcgcc aaccacaagt aaaattgcgc cacaaattgg gccgaacaat
    13621 gcccgtagtg gtacgccatg actgcggaag atatccagca ccagaccgcc aatcagctgg
    13681 ctggcgacca gtactgcaat agttgtcgcc gctcccacat tctgatatcc gctgatactg
    13741 gcaaagacaa aaaacgatcc cagcaagccg ggtataagtg tccaccagcg cacgctggac
    13801 accagttcgc caaaccccgc cataccttgt ttaaaccata aaatagaaac aaagagcaca
    13861 atccccacca gtgagttcag caacatggcg atgagaatgg tagaggaggt ctgggtgatg
    13921 cgcaccatca gcgtgttctg gactaccaga ccaattccgg cggcaatcaa aaaagcaagg
    13981 gtgagcgact gattcatcca atcgcgtccg gttcagtgcg ctcgtcgagt tgcaactgca
    14041 taaatgtcag atccagccaa cgaccaaatt tagtgcctac ctgcggcatt tgtgcggtga
    14101 tgacaaatcc cagcgactgg tggagatgca gcgaagcctg gttttgcgat tcgatcccgg
    14161 cgaccatgac atgcttcccg caatcccgcg cttcatcaat caatcggctt aacaatttac
    14221 gccccagacc tttgccctga tgatcgggat ggacataaac cgaatgttcc acggtatggc
    14281 gaaaaccatc gaaactacgc cagtcgccaa acgaggcata tcccgtcact acgccatctt
    14341 cttcacttac cagcaccgga taacctgcta tggtccgcgc ttcaaaccag gcaatgcggt
    14401 tatctgcatc caccgtttgg tcattccaga tagccgccgt atacaacacg gcgtggttat
    14461 aaatttccgc aatggcagca cagtcggctt tgcgggcaaa acggatggac atgtctggcc
    14521 tcaataaaat aatgatgatg atgttatcaa tactatcgaa aacagcccga agcaacggat
    14581 tcctcgatga aattattgcg tgttgcagag gggatgtgac ggctgcaaac aaaacccatc
    14641 ctgcgagcct gctactattg atggattcat gccaaaagca caggagagca ttatggggca
    14701 acaaaagcag cgtaatcgac gttgggttct ggcctcgcgt ccacatggcg caccggttcc
    14761 ggagaatttc cgtcttgaag aagatgatgt cgccacaccg ggtgaaggac aggtgttact
    14821 gcgcacagtt tatttgtcac tggacccgta tatgcgtgga cgtatgagcg atgagccatc
    14881 ttattcaccg ccggttgata ttggcggcgt gatggtcggc ggtacggtga gccgtgtcgt
    14941 gaagtcgaat catcctgatt atcagcctgg cgactgggtg ctgggctaca gtggatggca
    15001 ggactatgac atatccagtg gtgatgatct ggtgaaactt ggcgatcatc cgcaaaatcc
    15061 atcgtggtcg ctgggtgtgc tggggatgcc aggctttacc gcttatatgg ggctgctgga
    15121 tatcggtcag cctaaagagg gcgaaacgtt ggtggtagct gcggcgacag gaccagtggg
    15181 ggcgacggtg gggcaaatcg gcaaacttaa aggttgcaga gtggtggggg tagccggtgg
    15241 cgcggaaaaa tgccgccatg ctaccgaggt gttaggcttc gatgtttgcc ttgatcacca
    15301 cgcggacgat tttgccgaac aactggcgaa agcgtgccca aaaggtattg atatctatta
    15361 tgaaaatgtg ggcggtaagg tatttgatgc agtgctacca ttacttaata catctgcgcg
    15421 cattcccgtt tgtggcttag tgagcagcta taacgctaca gagctaccac ccggtccgga
    15481 tcgtttaccc ctgttgatgg caacggtgct gaaaaaacgt attcgcttgc aagggtttat
    15541 tatcgctcag gattatggtc accgcatcca tgagtttcag aaggagatgg agcaatgggt
    15601 gaaagaggat aaaatccact accgcgaaga aattactgac ggtttagaga atgcgccaca
    15661 gacgtttatc ggcctgctta agggtaaaaa cttcggcaaa gtggtgatcc gcgtggcggg
    15721 tgatgattaa agtttcaacg gcggcgtaag ccgccataaa aatccaggca aaaagcttat
    15781 tttcagcttt aattaactaa ccacgtcatt gttctgctgc taataacaac aaaggtgagt
    15841 ggtttatgat tattcacttt aatacaccag gtgaactcct tctgccatgc aggcagggtt
    15901 ggacagaaaa cacgcgaagg aattaaaatg cctggaacgg gaaaaatgaa acatgtcagt
    15961 ttgactctgc aggttgagaa cgacctgaaa catcagctta gtattggcgc attaaaacct
    16021 ggcgcacgcc tgattactaa aaatctggcg gagcaattag gtatgagtat tacacctgtg
    16081 cgtgaagcat tattacgtct ggtttcggtg aatgcgcttt ctgtcgcacc agcgcaagca
    16141 tttacagttc cggaagtggg gaaacgtcaa ttagacgaaa tcaatcggat ccgctacgaa
    16201 ctggaattaa tggcagttgc tctggctgtt gaaaacctca ccccgcaaga cctggcggaa
    16261 ctccaggaat tgctcgagaa attacagcag gcgcaagaaa agggcgacat ggaacaaatc
    16321 attaatgtaa acaggctatt tcgcttagcg atttatcatc gctcaaatat gcccatcctg
    16381 tgtgagatga ttgagcaact gtgggtcagg atggggcctg gtttacatta tctctatgaa
    16441 gcgattaatc cagcggaatt acgggagcat atagaaaact atcatctgtt actcgctgcg
    16501 ttaaaagcaa aagacaaaga gggatgcaga cattgtcttg ctgaaattat gcaacaaaat
    16561 attgctattt tataccagca atacaatcgt taaatcaaat gcccggcaaa taccgggcat
    16621 caaggtaatt actcaaatct ccacgcaata ttcatgccga caccataatt tcgtccgggc
    16681 gcaggttcgt aatatcgccc gtttgactca ttgacaatga cagaaccaac gtattcttta
    16741 tcgaataaat tatcgacact accaaataaa tccacagtta aattgtggta attgtattta
    16801 tacccggtga ataagccgac gagagtataa gacggcgctt tggccgtatt ttcatcatct
    16861 gccataatat cgcccatata acgcgcttcc gtgcctgcat accagccatc ttccggtaca
    16921 taacctatcg acgcaaagcc catattacgg gcgatcccag gcatccgatt accgttacaa
    16981 tcctgttcat tgcaaacatt gctgcgatag gtcgcatcaa gccaggtcca ggacgcgttt
    17041 acgcgaaaat ctcccgcgaa acgttgatcc catgccagtt cagcaccttg acgacgggtc
    17101 tttccagcat ttttgtaagt cgtacgccca ccgctactgc tatcgacaac aatttcatca
    17161 tcggtgtcgg tctggaacaa tgcgagacta agcagcccat caccaatacg cgttttactg
    17221 ccgatctcaa ttgtatcgtt ggtggatggt tttaaaccaa agttcatacc gctttgccca
    17281 tcagcgcgat aagacagctc attaatcgtc ggcgtttcaa aaccacgccc ggctgccaga
    17341 tagatattcc aggcatctgt cattgcatat tttagcgatc cggcaggtag ccatttgtga
    17401 taactggcat caccgctgtc atcgccgtta cccggagtaa cgtaatggtc gttggaatca
    17461 aaccacacgg agctgtagcg cacgccagca tccagcgaca gtttttcgct cagctgccac
    17521 tgcgtctgta aatagggatc gatgttccac atcagattgc gttcgtcgcg acgcaactca
    17581 cctttttgcc cgtactccgg catgccgcta ttcaggcgga agttattgta gcccttgcgg
    17641 ttttcactca tgttttcgta gttcaggccg gtagtgaacg tgaccggaac gcccagttca
    17701 ccacggtgtg tccagcggct gtctattccc tggtaatggc gttgcagggt aatcacgccg
    17761 cccgcatgtg acgggttaag ttgtggtgcc atgggtattg actggtactg ggtcgtttct
    17821 cgctctccgg catacatcat cacactcata tcatcccgcg aactcaggct acgttcatag
    17881 cgcaacccag cctgagtttg cttgatggtt tttcgcgtgt cgtactgttc tgcacgaggc
    17941 gcttgttgtg gattcgcctt ccattctgct ttggttagcc cacctgggtc atctgctttg
    18001 atatccacac tattgaaaat caggcttaat ttgctggctt catcaatgcg tacgcccagt
    18061 ttggcattgg ctaaattttt ctgtgcgcca ctatggtcac gatagccgtg ggtcgtaaaa
    18121 cgcgtggttg agacggtgta atcaacatcg ccgggctgtg tgccgtctcc cgttgcgccc
    18181 gttgctttca gcccatagcg ccagctgcca aaactgccgt agtaactatt ggcttcaatg
    18241 gttggtggct gttgtccggt ctgggtggtg acattcatta caccgccaga cgcgttgcca
    18301 tacagggcag agaaggggcc acgcagcact tccacatttt gcacactgct taaatcgatg
    18361 ttggatgttt gcccttgccc gtcgggcatg gtggcgggaa taccgtccac atacaggcga
    18421 ataccgcgaa ttccgtaagt ggaacgggag ccaaatccgc gaatcgacag ctgtaaatct
    18481 tgcgcatagt tctgccggtt ttgtacctgt aaaccaggca cgccggtcag tgattcggac
    18541 aagttaatgc gcggtgttgc caggcgcatc tcctcgccat ccaccacgct tactgctgca
    18601 ggggtatcca gttctgaaac cacctgcggc gcggcactga caatcatggt ctgttcatca
    18661 gcggcaaaaa caacggggga aaggacaagc agtgcgggca aaacggtctg tcggacggaa
    18721 aaaatcttca tgaaaaaagc caggttaaga atgggaaaac gccgtcatgg taatgaaatt
    18781 gtaaatttat ggaaaatgaa acggcacaat acgttaagta attgagaaaa ttgtagtcgt
    18841 aacggcaaga aatgctccac atttgagaaa ataatgatta ccattcccat ttataacaag
    18901 agcgtaacta tgattacgct tagcgaagca ttgtgaagca gcaaaaatat cggttcatca
    18961 aagggagtcg tcatgcattt acgtcatctg ttttcattgc gcctgcgtgg ttcattactg
    19021 ttaggttcat tgcttgttgc ttcatcattc agtacgcagg ccgcagaaga aatgctgcgt
    19081 aaagcggtag gtaaaggtgc ctacgaaatg gcttatagcc agcaagaaaa cgcgctgtgg
    19141 ctcgccactt cgcaaagccg caaactggat aaaggtggcg tggtttatcg tcttgatccg
    19201 gtcactctgg aagtgacgca ggcgatccat aacgatctca agccgtttgg tgccaccatc
    19261 aataacacga ctcagacgtt gtggtttggt aacaccgtaa acagcgcggt tacggcgata
    19321 gatgccaaaa cgggcgaagt gaaaggccgt ctggtgctgg atgatcgtaa gcgcacggaa
    19381 gaggtgcgcc cgctgcaacc gcgtgagctg gtagctgatg atgccacgaa caccgtttac
    19441 atcagtggta ttggtaaaga gagcgtgatt tgggtcgttg atggcgagaa tatcaaactg
    19501 aaaaccgcca tccagaacac cggtaaaatg agtaccggtc tggcgctgga tagcaaaggc
    19561 aaacgtcttt acaccactaa cgctgacggc gaattgatta ccatcgacac cgccgacaat
    19621 aaaatcctca gccgtaaaaa gctgctggat gacggcaaag agcacttctt tatcaacatt
    19681 agccttgata ccgccaggca gcgtgcattt atcaccgatt ctaaagccgc agaagtgtta
    19741 gtggtcgata cccgtaatgg caatattctg gcgaaggttg cggcaccgga atcacttgct
    19801 gtgctgttta accccgcgcg taatgaagcc tacgtgacgc atcgtcaggc aggtaaagtc
    19861 agtgtgattg acgcgaaaag ctataaagtg gtgaaaacgt tcgatacgcc gactcatcca
    19921 aacagcctgg cgctgtctgc cgatggcaaa acgctgtatg tcagtgtgaa acaaaaatcc
    19981 actaaacagc aggaagctac ccagccagac gatgtgattc gtattgcgct gtaatagata
    20041 tggcgagggt gaaaacggag agacttgcct gacttttatc acgccgcatg cggcacacct
    20101 tcgtcagtaa tctaaatttg cccggcacgt agccgggcaa ttttgcaatt agctcgccgt
    20161 ttcaggctta aacacaattt cctgtttttc ttcatcttcc tcgacgactg gcgcagtgct
    20221 gtgaatttca gcaacgcgtt tgcgcacacc aaaccagcct ataaccagca gaataccgat
    20281 aatcggcagc gccgcgatag tataagtccc gttcgggtaa tcgaacgcca tcagcacaag
    20341 gacactcagt aaaaacagta atgtcagcca ggaagtgaag ggcgcgccag gcagtttaaa
    20401 actgacatcc gctgctttgc cttctttaat cgctttacgc aggcgcatct ggcacacgat
    20461 gataaacgcc catgaagcga tgattcccag cgacgcgaag ttcaacacaa tctcaaatac
    20521 gcgcgatggc accagatagt tgaggaatac gccgacgaca tacacaacca gtgtcgccag
    20581 aatcccggca tacggcacat gctgacgact cattttcgcc ataaaactcg gtgcggaacc
    20641 gcccatcgcc attgagcgca gaatacgtcc ggtgcagtac agacctgaat tcaggctgga
    20701 gagggcagcg gtcagcacca caatgttcat aatgctgccg atatatggca cacccagttt
    20761 agagaaaaac gtcacgaacg gactttgccc cgcctgatac gcgctccacg gcaataacat
    20821 aaccagcaac accacggagc cgacgtaaaa caggccaata cgccaaatca cactgttaat
    20881 ggctttaggc accatggtct gcggatcttt acattctccg gcagctgtac ccaccatttc
    20941 aatggaggca aaagcaaaca ctacgccctg aatcaacacc agcgcaggca gcaaaccgtg
    21001 ggggaagaag ccgccattat cggtaattaa atgaaagcca gtggtgttgc catccagcgg
    21061 ctgaccacta ccgaggaaca ctgtacccac gaccaaaaag gtcacaatgg cgagcacttt
    21121 aataagcgca aaccagaact ccatctccgc aaaccatttc acaccgatca tattgatggt
    21181 gccaacgatg gtaagtgcag cgagcgcaaa gacccactgc ggcacgccgc caaacgcacc
    21241 ccagtaatgc atatacagag cgacggcggt aatatcaaca atccccgtca tcgcccagtt
    21301 gatgaagtac atccagccag caacataagc ggctttctca cccaaaaact cacgggcata
    21361 agagacaaaa ctgccactgg aagggcggtg tagcaccagc tcacccaatg cacgcagaat
    21421 aaaaaacgaa aacaagccac aaattaaata aaccagtgcc agtgctggac ccgccatttg
    21481 cagtcgggct cctgcaccta aaaacaagcc ggtgccaatc gcgccgccaa tggcgatcat
    21541 ctgtacctgg cgattgccca tcgctttgtg atacccctct tcgtgggcat taagccagcg
    21601 gcgtttcgcg gcgtgttgat ctgaagtgtc ggtgtcgtgt ttactcattg ctctccctga
    21661 ttgctttaat gaaaaagtca tataagttgc catgaacaat gtttattcat cctggcgatg
    21721 atagttatca ccattattct ttatgggtat aaaactatcg gcgcagcatc ctacccgtta
    21781 tcgataaacg atgcaaaaca tccccttaca atcctgaagg ggattaatac aactgacgaa
    21841 aaaatgacaa atccttttgc tggttaacct gtgtactgtc ctacacttaa tctttaaaag
    21901 attgtgaggg gcatatgatt aaggtgtatg gcgtacccgg atggggctct acaatcagtg
    21961 agctgatgct aacactggct gatattcctt atcagttcgt tgatgtcagt ggttttgacc
    22021 acgagggagc ttcacgcgag ttattgaaaa ccctgaaccc gttgtgtcag gttccgacac
    22081 tggcattgga aaatgacgag attatgacgg agacagcggc gattgcattg atggttctcg
    22141 atcgtcgccc ggaccttgcg ccaccggttg ggcgcgccga acgtcagcag tttcaacggc
    22201 tattggtctg gctggttgct aatgtctatc caacattcac tttcgccgat taccctgagc
    22261 gttgggctcc tgacgcaccc gaacagttaa agaagaatgt tattgaatat cgtaaatcgc
    22321 tttatatctg gctgaattca caattgaccc ctgaacctta tgcgtttggt gagcaactaa
    22381 cgctggtgga ctgttatctt tgcactatgc gcacatgggg cctgggcatg aatggtttca
    22441 ggataatgcc acgaatatca gtgcgattgc tgatgctgta tgtcagttac cgaaattaca
    22501 agaagtatta aaaaggaatg tcattatttg agtgggatac cagaagatta tgttat
//
LOCUS       DAPGRN010000076        22470 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPGRN010000076 DAPGRN010000000
VERSION     DAPGRN010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.076"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..1956
                     /locus_tag="RHW33_003082"
     CDS             49..1956
                     /locus_tag="RHW33_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDV1646562.1"
                     /translation="MKLISNKAKLKKILRNAAKYITPPPKLLPSEWCEANMVLVDGPQ
                     AGDKVKLLSFQKGMIDAPFLENKKKYVLMTSAQIGKTTILNGILFNQMANDPCNMIIG
                     QSTAKEMSQYLAGKIRPSIEACDALKDVVTDKNDRNAVNNNNQLQLKTNHFLYMVSLT
                     SPSTLRGKTAKVGLLDEIDAATASEEGDPVALAANRLTTFGDEGRLVVSSTPTSKLGS
                     INQQWLSSDMRMFFVPCPHCGRHQVIEWENVQFEWRNIDGKNLPDPDTARYICPHCKN
                     AWTEGERIRAVAQGEWRATRESEVAGFWISRLYSPFSTIRACVVDFSHAWQSFDLQSF
                     FNTVLGKVYDDQDTAVEANELEQLKTDVSIENIPDDVIFLCSGIDQQLDRAESTILGV
                     AKDKVYILDHRSFYDHNCERYESPVWDRLVNFKKTKFYNVNGERVPMLASFLDTSNGR
                     FTQAGYRICGKWKNLHAIKGSSSGNAPVIPVKPTRTGGHELLMLGVNVGKSAVREMLV
                     RNLKDNPHIGLEISDTVPDDYLDQLLSESIKRTTTGVRWVKNPGSTRNEALDCLVYSY
                     AASRYVLSKMSWDKLIAMKDSLNRVVEEPVEAPKSQSDEQIEETKPITRPQRQNIARR
                     PNRGRSWVTSF"
     gene            2029..2523
                     /locus_tag="RHW33_003083"
     CDS             2029..2523
                     /locus_tag="RHW33_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646563.1"
                     /translation="MSLELIPLVIRKGEKITLANEEGVTIQVGNSKGIIYQVDDSPAN
                     HEIKTLDFAEGKYTIVTTLEEELVSMQELTVLPVFAKQSKKEYLRETIATIEQVIFAR
                     LSGDEAALSQMTVKGNTFAYESLAVLQTLKVDYERQLSKLIQAERRKQGISPIKNIKL
                     RLTR"
     gene            2534..4045
                     /locus_tag="RHW33_003084"
     CDS             2534..4045
                     /locus_tag="RHW33_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1646564.1"
                     /translation="MFNLFRRKKAVETPVKTNHRQQQQKIFIDKQVEKFQKDLSKRSL
                     GLVGDRIDGQLQQDTITGTFNKALKSNGKRLYDQGRTLALNTSVGSRYTQYITDMVVG
                     TGLDPKPSVVKSNGKLDSALNKQIENAFWKWAQNAKRFSRNGRFNFRELLVMAERERV
                     MGGECFIVLTKENNELNVSILSADKCDWTLNREVSKDRAIFQGIEYDVETMRPVAYWF
                     RKVNLLTQTYTGDHYRVEASQVCHYYQPLAAESLRGVTDFLPVIKDIAHQDAFRLTAI
                     VQKRIAASSMGFIERPKDSGDDFDTGEDDEQYQAPEVVQDFAPGTIQELPEGATIKSI
                     QATQSGDDFNSFNDAMFTSVSMGLGVFKQGLTGDTSQINYSAARYGELLQRTRVKALQ
                     NKLIETVVLPIFEAYLRHYSARGIVPIRITAIPHIIDNTTIIRPRFESVDPIKDVNAE
                     IALIDKGLKSRTAVILERGDDPEKVFSEIQAEKSALNIIVDGEGEEKNSPADP"
     gene            4091..6097
                     /locus_tag="RHW33_003085"
     CDS             4091..6097
                     /locus_tag="RHW33_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDV1646565.1"
                     /translation="MLKFRRDLNGYGGVINEGQNDQYEFEIAFSSEQPYQRQFWDEQN
                     QEMVVLDEILVHTPEAVDLSRLNNNAPLLFNHNFDNHIGVVCNARIDADNVGRALVKF
                     SKHGTLANDIRNKVIEGTMEKISVGYDIKEYHIDYTKGQLIVTKWIPHEVSWVSVPAD
                     DTVGLNRSLNTITVNLEAKRDMTKEQIEEIKEEQEPAQVEETPVEENKEPEVEETQER
                     QVEENEEDENLEDGKDAEHPESVDDDSSTVREAEEVKEEREAAPVEEEKIEEVAERSE
                     EDELEIREIARELNIDDEELKRALAIKDMTPEAFRTKALNKIATAQRNNEQQIKDSKM
                     EKTFDLNNVIRSLVDGEALGAHEAEFSAMAATATMQRGRAARGGSVFVPTAAMRAAAD
                     GNTKATLTAVTDEKLLNDSYVAMLLPESVLGRLGVKVLSGLTSPTAIPKMTASSVESF
                     GFVDENGAAPEGKAEFANVKMAPKTFAGGNPISRASLKTVPGIATLITDHINQAVRIK
                     LEQLILSDKENARGPAGVIKQLVDAGRVEKKAAFSYKDFLKEIAKLTDAGVPAQAIKF
                     AMSGATAAELESTLKDNGVSGYIIENGKLAGYDVVTSGVIPVDHIVLGSFDAITIGEW
                     GGLELDIDDTTYRAQSAIVPRIWVDLDYVVTQPEALKVLHISAE"
     gene            6290..6580
                     /locus_tag="RHW33_003086"
     CDS             6290..6580
                     /locus_tag="RHW33_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646566.1"
                     /translation="MFKLSESQLSRMFKSAPVFSVDGGKSIRAYHEITTTDEQGVMTE
                     TEFLFCREGDLKQGDIVTVENQRFKVQYVKRNGDNTCDCFITLAGGTHARYR"
     gene            6564..6986
                     /locus_tag="RHW33_003087"
     CDS             6564..6986
                     /locus_tag="RHW33_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646567.1"
                     /translation="MLATVNNMPRLKIKRALQDIIEQDLGLALNVEQTQQGFSDDVVC
                     WITGMNETYTRVRGGNAMQAECVIEMQLYSQIHETKIHEGICQIIQIQPDNPRFKDLG
                     FAISDITPVASNTDYDDDSSDGGIVGTLSLKFSYVARF"
     gene            6996..7829
                     /locus_tag="RHW33_003088"
     CDS             6996..7829
                     /locus_tag="RHW33_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646568.1"
                     /translation="MNITQDNLDIFTGSHVEVSVSTMVDNQPDFFDPAFSPIENIAAF
                     PTITESTEIQTLEEYDHDVTGKLAGYRKLESTTLTVNRVLDDEHQAMLMKAVEDKTPL
                     RFRMFYVVNSGYSAANTGYYVIFDAYVTSHKTRGSDNKVTTIEFKLDPDGGILDRGIA
                     TEGKILRQGDFGLGAGVHPFTGPIDSESLTGNRFVTYQGSSASNPFSADTSLIHVQPN
                     EDGGWQLTCTASGAPRLRVRTVQKDGNSGWVKVYSTEEKPTPAEIEAVSIHDRIDFGE
                     Y"
     gene            7903..11430
                     /locus_tag="RHW33_003089"
     CDS             7903..11430
                     /locus_tag="RHW33_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HDV1646569.1"
                     /translation="MQSIQFKRTNVSGKKPTPEQLQVGEVAINLKDHVIFTKDRDHEV
                     VQISVSPETHAALESKVDANKQELDSTIAVNDRNIHAKVDEIKQTTDATIAANHAEIN
                     NKVDVIKRETDATIEANKNKAASDLAGVKAELSDTINANKNAAAVATQELDTRINKKV
                     DDIKSRTDATIASNDKAINDKVDLIKAETDRTIAANHAYAENQLTDTYNNLTGVIAAN
                     KQEAADNVAALTRDVEAKNTAIHSKVDNNKSYTDSELARLESRIDAADGSSDGKYIKK
                     HVNTYTDGYLLSKTANYFDDPSARNLDYFGAFRMNDLAGHIAMILHVPHPSGVNHARG
                     FEFTYGSNPVHTVRTYGYDELGHLAYSHRMYHEGDKPTPAEIGAYSKAEIDRLFQKTL
                     NFGIEGGWFKLGTLTMPQQHGRTAKIRLVGGNGYNVGHNGQANIIELVIRCGNNSPKG
                     VVFNAYYTIWHYEQHFCAIPTDGDNYDLYAYYGAHTGFVLAEYQVSSGGVSLNLLDTP
                     EYLGGEKPVADEIFDALNISSFNNFSNRGTLNFAGNHQGQYDIEHLNVQQTNSKKMLR
                     RFRSSGPATIWHETVDDNQYRLATGYEDTNQELLLTATSGLHVKKLTLDGGVTGNSGI
                     DIRRGPNESSHFNFMDYRTGQDVRNGWFGFGDGQTKDFIWWNDNGQNSINLIEDGELH
                     ITGGKGQKIVMNSEVALSENARLAVKGGNYGLIFRNDGSDFHILTTGLGDSFGPWNTR
                     RPFSYNFEDGGLYLGGTETSRCLHLGIDGSTRLEDNLFFKAGSRQSMDYIEMIHWGNS
                     NIARDNVLSMKDSKGFLAEFERVGGTNGVKTRFFGETFTDGTLYLNQMNNSSERFSIN
                     NWGNSEVGRSAVMEIGDSKGYHFYTERRTDNSLAFDVAGAFTVHGPNGITIKNSAGAR
                     HIWFRDDSDTEKAVIWATDDGRLHIRNNNGGSFSHHFQGAMIKLEGRVPYAADQGLIR
                     GEVDGGAYVAWRDRPAGLLVDCQKSIDSAHAIWKAVDWGRQYIAAMDVHCPGDGNNTA
                     AAVLHVQGADYQFHASGEFHATGNGNFNDVYIRSDRRLKINVEDYEENAVDKVNKLKV
                     KTYDKVKSLNDREVIGHEIGIIAQDLQEVLPEAVKTAKIGGLDNPEEILTISNSAVNA
                     LLIKAVQEMSEENKLLRERLAAIEAKLFG"
     gene            11470..11766
                     /locus_tag="RHW33_003090"
     CDS             11470..11766
                     /locus_tag="RHW33_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646570.1"
                     /translation="MGYWVSSEINTKIDALKWHVFDIPNGYGTARAGVAVMRHNAGGG
                     WSDAHINVDGLTVARRRGGTNWGSTINSFVPAGCTVSFGLGGEGGVEYFKFMEF"
     gene            11769..12074
                     /locus_tag="RHW33_003091"
     CDS             11769..12074
                     /locus_tag="RHW33_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646571.1"
                     /translation="MAGWINQRMSNAISIWANGGYFDIPNGWVTDSCGIVFAHMEAIN
                     GAGDLDSELVVNGLIESGHHAGDAGSWGASSLVGAGATVSFTLGKGSLHYFKFRRMH"
     gene            12074..12376
                     /locus_tag="RHW33_003092"
     CDS             12074..12376
                     /locus_tag="RHW33_003092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646572.1"
                     /translation="MAFWVDGALGNRAGKANQGWFDIPNGWTADAFGTISFCAHSSGG
                     GGGDSELFIHGFCVSGSHKGHDWGQIHSCIAPQGAVITVSTNRGIAHLRYRRLSNR"
     gene            12430..12903
                     /locus_tag="RHW33_003093"
     CDS             12430..12903
                     /locus_tag="RHW33_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646573.1"
                     /translation="MTTKFDIFSGGLVGLFLQEDVNNKDLDAETYLEIPECAAFPETG
                     VERSTIDVPNFSGAYNRKLVGRMSVPDIELSINYMPGNEVHEKLIKATEDGKRVQLKI
                     AYYVDATKKSGIQIAYNGFLSKTTMTGGEDAVVGRTFTFVVDGAPVKQAVFQKVV"
     gene            12986..13294
                     /locus_tag="RHW33_003094"
     CDS             12986..13294
                     /locus_tag="RHW33_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome"
                     /protein_id="HDV1646574.1"
                     /translation="MNINEMLKALSPKRESLTIGGFTFYARPMSVKEFNEHVFNTDKE
                     DRDERSILRCIEDEDGKPVFESMEQVKALYTNVRSELIGLVAQASLMQDPAVIENEVK
                     "
     gene            13438..13665
                     /locus_tag="RHW33_003095"
     CDS             13438..13665
                     /locus_tag="RHW33_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646575.1"
                     /translation="MQNAMLQYSMYMTSQGMTRETARKLKPSQFQLIREEKLFKTKEE
                     LEELARKKEEERKAAMLNMFDPSLLEKLRSG"
     gene            13677..16106
                     /locus_tag="RHW33_003096"
     CDS             13677..16106
                     /locus_tag="RHW33_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646576.1"
                     /translation="MTRHIVTIEGDNKGLRRSTNEAADLLDSLSEKASNIDFGGGLSG
                     LTGSLRGIAGSAGLAAGGIGLVATAVVAAAKAGAEYVKQYSEVSKATGLSIESLQRLE
                     KEFSGTGLTVEKFGDINKDTLDKMGDAWANGGGIADDLESVGLKLENYAHFMTDPQGG
                     MKAAIQVFYDMKKAGKSMAEIKFMMESLASDSSHMTSQLEKYNTAQEAMIAIQNQSVN
                     VTEENAKKYDKFSQNINKLENNLKGVGMTITGPLVDSLNWLFDWFNIDWEKSSLFRAL
                     DRLNKEGKTATGGILNTNHKDAQKIIDKYNKEKRWNNLADWEKAAIRGAGVDPRTAGF
                     DVEGFKKRFGDSYKKNGSLIVVDNGEHLTRKADPNRDLTIPTTPTRPASLGKSGNEKK
                     AEEEARKKAEEAAKKAKEAAEKAQKEREDAIKRLNALDVKLQGQVAASIASQNKQLES
                     SLKDLDTALDLGLISQQDAAAKRQSLIDQNTENVYKMILGADPVDALNALTQLQQIRD
                     NELESHKRLLDGKAISYEEYMRRVNDTEQSYSQIEDSLHGMDGFKTNQLTNSLDYQDS
                     NNPFAKFNAIDKEKSEAEQDYKTDKLKIDGITDPAKRMEALEKLNENHQKRMAAIEKK
                     YADARQSIADDMYGGFAAAMTLFGQENTKAMQMAFTAHKAFSIGQATVNMWTAATDAW
                     NDPTNVTTGQKIAAAALAVSQNMGSIANIKSTNVSGMAHDGIDNIPREGTWLLDKGER
                     VVDQRTNGDLKDFLSAQKSGGGNSQPIEVNAPLNINGNVNSSDKMVMDAIKRHAKLVA
                     QAVEDAQRRKM"
     gene            16157..16717
                     /locus_tag="RHW33_003097"
     CDS             16157..16717
                     /locus_tag="RHW33_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646577.1"
                     /translation="MFKSKNIKITDFTLKSKQPFFKAQSISGKFQRRFTGIHFYEAEF
                     TANFMAQDINEVKEFVARHLFGRPFSVPLSYFSKYTGDVRQMVTAAAGTARGGRKVRI
                     SNFTGTLKAGTIIQFENHKKIYTITEDVKSGGEMKLFPNLRQNVLAGEVIKYQNVEGE
                     FVLKTENIDWKIAQIGKMKFELVENV"
     gene            16801..17370
                     /locus_tag="RHW33_003098"
     CDS             16801..17370
                     /locus_tag="RHW33_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HDV1646578.1"
                     /translation="MSRLTLPQLFSTGSMFHIIEVITASGDVLRLTDGYFDLDYNGFT
                     YLATGDFLQISSNTEEKEINNNGINVTVSNVREEYITLIRNKQFDKSDVKIEMVFLNP
                     NTGKVETTYPVFRGVVDSIGINIEHEDDECKNESEFQLNSIWEVLDKNARSHASDGIH
                     RSYAGNENDLFFSRAGKWQSESKWFSSKK"
     gene            17445..18218
                     /locus_tag="RHW33_003099"
     CDS             17445..18218
                     /locus_tag="RHW33_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646579.1"
                     /translation="MQKFHYVYHTTIKSNRSGKIYHYVGKRTTTNLDDGYVGSGKVIK
                     MMKRKNKESETPIYEFEVVRSKLFDTAEEAFECEILLVEAAREKWGRGVCLNLAPGGV
                     GGFDGGEHHPMYGKKHKPETIEKFRKNNAGEGNPMFGRVSPKRGRKITNPETLAKMSA
                     AMKGVNNPRYGAIVTEETRLRISKALSGRKITDPVEKANRTKAIRESKGQGCWQHYDE
                     IRRVWIENGKPGAVKLSQIMVSLGHPKYNLRRMYEDFKK"
     gene            18250..18684
                     /locus_tag="RHW33_003100"
     CDS             18250..18684
                     /locus_tag="RHW33_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646580.1"
                     /translation="MEVFNMLKTRLITDYINSLIGQEFVQGENDCNLIACKIIDILAG
                     TDLYNSLYKKYSTKEEGLKICKELSGYSNILQPIKKHFKLVTDDLQDGDLLVTAHKLG
                     NRKYYSVVPHYSGYGLVEEDGIWMTIPVSDIDYEQVYRFGGE"
     gene            18684..22430
                     /locus_tag="RHW33_003101"
     CDS             18684..22430
                     /locus_tag="RHW33_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDV1646581.1"
                     /translation="MGFEILVGAVIAGASAGMAAAATFSVMTAVAIGMAAGAMTLIAS
                     TVGAPKTPKVQSPDNAVTLGTSNDPKTVLPVLFGTTRTGAICVYKAISQRENNKLVQI
                     FAIAEGEIDHFKALHIDNKNVLISQNMTIRDGILDKGNIKDEYRKVLEVEFRTGKNPN
                     TALDLAKHHLGADWDDRYQGNGIATMCIVLRRDDKSLAAGVDILQPNSQVAVDVMGLK
                     IRNLETNAIEASTNGVDQIFHYLTNEKYGLSVPIENINVDSFLKVRKQVRQMDLHSNG
                     ACDPNASFKENLTSLMQTFGGVMFESFGRITLKLDAPDIVKHTFNEDNIMMGKVSLKT
                     GGTNGYFNTINAMYQEPSIDYSEQMLRYPADAENDATVRADGRIIAKDVEYRFVKSKD
                     QIDKLASIERNKSRITQVISFMTTDAFTAEVWDVISVTYDELKLNNSLWRITAIDRSI
                     DSGIAGMMTITATEYNSQVYTDLNYAATPDNRPTGLPDSMTVQKPTNFRIKATGETIH
                     GKNVTLTWDAPEDFNRYGFQIDYRVSGSPNWIKLGQTSQQIFNINALAKDRSYDYRVC
                     AFGIIARSEWVELVNQNPEVTYELPTPVIRIKNQGSTPGTFEGNDLIIEWENQQQLDV
                     MINGETNKFSDLFEAYIIKVTNKAGKSIQYRTRDPESWTYTLDMNQFNGLSRELTVEV
                     SVKGYNNSESAPARLVAINPQHKPMKGFSARGGFNTAFVSWADDVEHDYAGSIIQYAT
                     DNTFSDARAVTTNSVSHTSFDLADGDYYIRGAHYDIFGMDDAVWSEPYFMQMKSTISW
                     DDQDKEALEDLIGLQDRLDETIADAIAQAGANADAKIDAMHKQITTETGQTVQASADT
                     LKSLIATSEKASSTKIDQVKAELKGDITNEVSASATTLKQAIATSEAASASKIDQVRV
                     EMDGKIAGVNQEADVKIDALKGTINSKYNLAVNADGRVAGIHMSATNDPAEPTRIIFT
                     ADKIAVAPQNGSEVCPFGIEDNKVYLDNAMIRNAAIGTAQISDASITTAKIGTAQING
                     AHIQHAQIGTGHIIDGSIDNAKIGNYIQSYNWNGNDGWYIGKDGTCHFRHANIRGHLV
                     ADSGEMNNVTINSSCRILGMLDANQVRGDFVKVVGRSFPHRQPNWPDGAITVRVEDDH
                     PFDRQIVIPAIHHNGSVRRGESSDHWDTCRLIVKKNGGVLYDRTATSGCMYSGVIDMP
                     AGHGAVTMTFEVYCKGVNNYNPSAYISDLTVMVTKKATTGITVS"
BASE COUNT         6851 a         4426 c         5150 g         6043 t
ORIGIN      
        1 atcaacaatt taataaatac cttcatattt cgtaatgagg gtaattacat gaaactaatt
       61 tctaataagg ccaaattaaa aaagattctc agaaatgcag ccaaatatat tacgccaccg
      121 cctaaactcc ttccttctga atggtgcgaa gcgaatatgg tgctggtaga tggtcctcaa
      181 gcgggggata aagttaaatt gctgtcattc cagaaaggta tgattgatgc tcctttcctt
      241 gagaacaaga aaaagtatgt cctgatgacc agtgcgcaga tcggaaaaac taccatccta
      301 aacggtatcc tgtttaacca gatggctaac gatccatgca atatgattat cggacaatca
      361 accgctaaag aaatgtctca atatctcgct ggtaagatcc gaccgtctat cgaagcatgt
      421 gatgcattaa aagacgtggt tacagacaag aatgatcgca atgcagttaa caacaataac
      481 caactccagc taaaaacgaa tcacttcctg tacatggtat cgcttaccag tccatcaacc
      541 ctacgcggta agaccgcaaa ggttggatta cttgacgaaa tcgatgctgc tacagcctca
      601 gaagaaggtg atccggtggc gctggctgct aaccgtttaa ctacctttgg cgatgaaggt
      661 cgattagtcg tatccagtac cccgaccagt aagctcggaa gcattaacca gcaatggtta
      721 tcaagtgata tgcgtatgtt ttttgttccg tgtcctcatt gcggtagaca ccaggtgatc
      781 gagtgggaaa acgtacagtt tgaatggcgt aacatcgatg gtaagaattt acccgatcct
      841 gataccgctc gttatatttg cccccattgt aagaacgcat ggacagaagg ggaacgaatc
      901 agggcagtag cgcaaggcga gtggagagca acccgtgaaa gtgaagtagc aggattctgg
      961 attagccgtc tgtattcacc tttcagcacg atccgcgctt gtgtggttga ttttagtcat
     1021 gcgtggcaat cctttgattt acaatcattt tttaacaccg tattaggtaa ggtgtacgac
     1081 gatcaggaca cggcagtaga agcaaatgaa ctggaacaac tcaaaacaga tgtttctatc
     1141 gagaatatcc cagatgacgt gattttcttg tgttcaggaa tcgaccagca attggaccgc
     1201 gcagaatcta ccatcttagg tgtagcgaaa gataaggttt acattctgga tcaccgcagc
     1261 ttttacgatc ataactgtga acgatatgaa tcacctgttt gggatcgtct ggttaacttc
     1321 aagaaaacca aattctataa cgttaatggt gaacgtgtgc caatgctcgc cagcttcctt
     1381 gatacatcga acggtcgatt cactcaagcc ggataccgta tttgtggcaa gtggaagaac
     1441 ctacacgcga tcaaaggtag ttcatccggt aatgctccag tgattccggt taaacctacc
     1501 cgcacaggtg gtcatgaatt gcttatgttg ggtgttaacg ttgggaaatc tgctgttcgt
     1561 gaaatgctgg ttagaaacct gaaagataat cctcatattg gcttagaaat atcagacacc
     1621 gttcctgatg attatctcga tcagctttta agtgaatcca tcaagcgtac tactactggc
     1681 gtgcgttggg tgaaaaatcc aggtagtacc cgaaacgagg ctctcgactg tctggtttat
     1741 agctatgctg cttctcgcta cgttctttca aaaatgtcat gggataagct cattgcaatg
     1801 aaagacagct taaaccgtgt tgtcgaagaa cccgtagaag ctcctaaatc tcaatctgac
     1861 gagcaaatag aagaaactaa gccaatcaca cgaccacaac gtcaaaacat cgccagacgt
     1921 ccaaatagag gccgtagctg ggtaacatcg ttctaataac tcgccgtcct tcggggcggc
     1981 ttactcctaa atattgttaa tccaataaca attaaataag gggtaattat gagtttagaa
     2041 ctaattccct tagtaattcg taaaggcgaa aaaatcacgc tggcgaatga agagggtgta
     2101 acaattcagg taggaaatag taaaggtatc atttatcagg ttgatgattc tccggctaat
     2161 catgagatta aaaccttaga ttttgccgaa ggtaaatata ccatcgtaac cactttggaa
     2221 gaagaactgg tatcaatgca ggaattaact gttttgccag tattcgccaa acaatccaaa
     2281 aaagaatatc tgcgggaaac tatcgccaca atcgagcaag ttattttcgc ccgtttatct
     2341 ggggacgaag ccgcattatc tcaaatgaca gtgaaaggga atactttcgc ctatgagtca
     2401 ttggctgttc tccagactct aaaagtcgat tatgaacgtc agttatcaaa attaattcaa
     2461 gccgaacgac gtaaacaggg aattagcccg attaaaaata tcaaattacg tcttacgcga
     2521 taaggggtaa atcatgttta atctttttcg acgcaaaaag gcggtagaaa ctccagttaa
     2581 aactaatcac cgccagcaac aacaaaaaat ctttatcgac aaacaagtag aaaaattcca
     2641 gaaagatttg tctaagcgtt ctttgggctt agtcggtgat cgaattgatg gacagcttca
     2701 acaagatact atcaccggaa ccttcaataa ggctctcaaa tcgaacggta agcgccttta
     2761 tgatcagggt cgtactctgg ctttaaacac ttccgtaggt agtcgctaca cgcaatacat
     2821 caccgatatg gtggtaggta ctggcctaga tccgaagccg tcagttgtta aatcaaatgg
     2881 caaacttgat agcgcactga ataagcagat cgagaatgct ttctggaagt gggcgcagaa
     2941 tgctaaacgc ttctctcgta acggtcgctt taacttccgt gaactgctgg taatggctga
     3001 acgtgagcgc gttatgggtg gtgagtgctt catcgtttta accaaagaaa acaatgagtt
     3061 aaatgtttct atcctgtctg ctgataagtg cgactggaca ctcaaccgtg aagtaagcaa
     3121 agaccgtgcg atatttcagg ggatcgagta tgacgtagaa acaatgcgtc ccgtagcata
     3181 ctggtttcgt aaagtcaatc tactgactca gacctacact ggtgatcatt atagagtcga
     3241 ggcttcgcaa gtatgccatt attatcaacc acttgcggct gaatctctgc gtggtgtgac
     3301 tgacttccta ccagtgatta aggatatcgc acatcaagac gcattccgct taactgctat
     3361 cgtccagaaa cgtattgcgg ctagttctat gggctttatc gagcgtccga aagattctgg
     3421 tgacgatttt gatactggtg aagatgatga acaatatcaa gcgccggaag tagtacagga
     3481 ttttgcaccg ggtactattc aggaattacc ggaaggggca acgatcaaga gcatccaggc
     3541 gacgcaaagc ggtgatgact ttaacagctt caatgatgcg atgtttacta gcgtatcaat
     3601 gggcttaggc gtgtttaaac agggcttgac tggcgatact agccaaataa attacagcgc
     3661 cgcacgctac ggcgaattac ttcaaagaac gcgcgttaaa gcactgcaaa acaaattaat
     3721 tgaaacagtg gtattgccaa ttttcgaagc atatctacgc cattattccg cgcgtggtat
     3781 tgttccgatt cgtattactg caattccgca tattatcgac aacactacta ttattcgtcc
     3841 gcgttttgaa tccgtcgatc ctattaaaga cgtaaacgcc gagattgctt taattgataa
     3901 aggacttaaa tcacgtactg ccgttatatt agaacgtggt gatgatcctg aaaaagtatt
     3961 ctcagagatt caagccgaaa agagcgcact aaatattatc gttgatggcg agggtgaaga
     4021 aaaaaattcc ccagccgatc cctaataacc aacgggggcg caatgccccc aattaattaa
     4081 aggtgattaa atgcttaaat ttcgccgcga tcttaacggt tacggtggag ttattaacga
     4141 agggcagaat gatcaatacg aatttgaaat tgctttctcc agtgaacagc cttatcagcg
     4201 ccaattctgg gatgagcaaa atcaagaaat ggtggtatta gatgaaattc tggtacatac
     4261 accggaagcg gttgatctgt ctcgtctgaa taataacgct ccgttgctgt tcaatcataa
     4321 tttcgataat catattggtg tcgtttgtaa cgctcgaatc gatgcggata acgtaggtcg
     4381 tgctctggtt aaattctcta agcatggcac tttggctaat gatattcgta ataaagtcat
     4441 tgaaggtacg atggaaaaaa tttctgtcgg ttatgacatt aaagaatatc acatcgacta
     4501 caccaaagga caattgattg ttactaaatg gattcctcac gaggtttcat gggtgtctgt
     4561 tcccgcagac gacaccgtgg gtctgaatcg ctctctaaat actatcacag ttaatttgga
     4621 ggctaaacgc gatatgacta aagaacaaat cgaagaaatc aaagaagaac aagaacccgc
     4681 tcaggttgaa gaaactccgg tagaagaaaa taaagaaccg gaagtagaag aaactcaaga
     4741 gcgccaagtt gaagagaatg aagaagatga aaatctcgaa gacggaaaag acgctgaaca
     4801 tcctgaaagt gttgatgatg atagttcaac tgttcgggaa gcagaagaag taaaagaaga
     4861 acgtgaagct gctccggttg aagaagaaaa aattgaagaa gtggctgaac gttccgaaga
     4921 agacgaatta gaaatccgcg agatcgcgcg ggaactaaat attgacgacg aagaattgaa
     4981 acgcgcattg gcaattaaag acatgacgcc ggaagcattc cgcactaagg cactaaataa
     5041 aatcgctact gctcaacgta acaacgaaca acaaattaag gactctaaaa tggaaaaaac
     5101 ttttgacctg aacaacgtaa ttcgctctct ggtagatggt gaagctctgg gtgctcacga
     5161 agccgagttt tctgcaatgg ctgctactgc aactatgcag cgtggtcgtg ctgctcgcgg
     5221 tggctctgta ttcgttccga ctgctgctat gcgtgctgct gctgatggta acaccaaagc
     5281 tactctgacc gctgtaaccg acgaaaaact gctgaatgat tcctatgtag cgatgctgtt
     5341 gccggaatcc gttctgggtc gtcttggtgt gaaagtcctg tctggtctga cttcgccgac
     5401 cgctattcct aaaatgaccg cttccagcgt tgaatctttc ggcttcgttg atgaaaacgg
     5461 tgctgcgccg gaaggtaaag ctgaatttgc aaacgtgaaa atggctccta agacgtttgc
     5521 tggtgggaat ccgatcagca gagctagcct caagaccgta cccggcatcg ctaccctgat
     5581 cactgatcac attaaccagg ctgttcgcat caaactggaa caactgattc tgtccgacaa
     5641 agaaaatgct cgtggtccgg ctggtgtgat taaacaactg gtagatgctg gtcgcgtaga
     5701 gaagaaagct gctttctctt ataaagactt cctgaaagaa attgctaaac tgaccgacgc
     5761 tggcgttcct gctcaggcga tcaagtttgc aatgagcggt gcaactgctg ctgaactgga
     5821 atctaccctg aaagataacg gtgtttccgg ttatatcatc gaaaacggta aactggctgg
     5881 ttacgacgta gttacttctg gtgttattcc ggtagaccac atcgttctgg gatcgttcga
     5941 cgctattacc atcggtgaat ggggtggtct ggaacttgac attgatgaca ctacctaccg
     6001 cgcacagtct gctatcgttc cgcgtatctg ggtagatctg gattacgttg taacccagcc
     6061 ggaagcgctg aaagttcttc acatctccgc tgaatgaact cctgtagaac catctgaacc
     6121 ttcccccgat ttgggggaag aaaatctgat tcctgaaccg gaagaagaat cccagacggt
     6181 taaggcgaaa gccacagcta aaaaacagcg taaaactaaa gaataataat tagccctgcc
     6241 ttaacggtgg ggcttttttg tatgtaaata ctccataaag ggggtaacta tgttcaaatt
     6301 atcagaatca caattatcaa gaatgtttaa aagtgctcct gtattttcgg tggatggtgg
     6361 taaatcaatt cgtgcttatc atgaaattac tactaccgac gaacaagggg taatgacaga
     6421 gacagaattt ctattctgtc gtgagggaga cttaaagcaa ggtgatattg tcacagtaga
     6481 aaaccagcgt ttcaaagttc aatacgttaa gcgcaatggt gacaatactt gtgattgctt
     6541 tatcacttta gcagggggta cacatgctcg ctaccgttaa taatatgccg agactgaaaa
     6601 tcaaacgcgc cttgcaagat attatcgaac aagatttagg tctggcttta aacgtagaac
     6661 aaactcagca aggctttagt gatgacgtgg tttgttggat caccggaatg aatgagactt
     6721 acactagggt ccgtggtggt aatgcaatgc aagctgaatg cgttatcgaa atgcaattgt
     6781 attctcagat tcatgaaacc aaaattcatg agggtatttg ccagataatc cagattcagc
     6841 cggataaccc acgttttaaa gatttgggct tcgctatttc agatatcact ccagtagctt
     6901 ctaataccga ttatgacgat gattctagtg atggtggtat cgttgggaca cttagcctta
     6961 aattttctta tgtagcgcgt ttttaagggg taataatgaa tattacacaa gataacttag
     7021 acattttcac ggggtcgcat gttgaagttt ccgtgtctac tatggtggat aaccagccgg
     7081 actttttcga tcctgcattt agtcctatcg agaacatcgc agcatttccg acgataaccg
     7141 aatccacaga gatccagacg ttagaagaat atgatcatga tgttactggt aagttggctg
     7201 gctatcgtaa acttgaatca acaacactca cagtaaaccg tgttctggat gatgaacatc
     7261 aagcaatgtt gatgaaagcg gtagaggata aaacaccttt acgcttccgt atgttctatg
     7321 ttgtgaactc tggctatagt gctgctaaca caggatacta cgtcattttt gatgcatacg
     7381 tcacatcaca caaaacccgt ggtagcgata acaaagttac taccatcgaa tttaaattag
     7441 atcctgatgg tgggattctc gaccgtggta ttgcaaccga aggtaagatc ttacgtcaag
     7501 gtgattttgg tttgggtgct ggtgtacatc cattcactgg tccgattgat agtgaatcac
     7561 tgactggcaa ccgttttgtg acctatcagg gatcgagcgc atctaatcca tttagtgctg
     7621 atacttcact aattcacgtt caacctaatg aagatggtgg atggcaatta acttgcaccg
     7681 cttccggcgc accacgttta cgagttcgca cagtccagaa agatggtaat tctggatggg
     7741 tgaaagtgta ttccacagaa gaaaaaccga caccagccga gattgaagcc gtttctatcc
     7801 atgaccggat cgacttcggg gaatactgat tgcttctcta aataaaacat gacgcgggag
     7861 gttaacgcct cccttattcc tgttttatag tgaggtgatt cgatgcagtc gatccaattt
     7921 aaacgaacaa atgtttctgg caaaaaacca acgccggaac aattacaggt cggtgaggta
     7981 gcaatcaacc ttaaagacca tgttattttt accaaagacc gagatcatga agtagttcaa
     8041 atctctgttt ctcctgaaac tcacgcggca ctagaatcta aagttgatgc taacaaacaa
     8101 gaactggata gcacaattgc agtaaatgac cgcaatattc acgctaaagt agatgaaatt
     8161 aaacaaacta ctgatgcaac catcgcagca aatcacgcag aaattaacaa taaagttgat
     8221 gtaattaaac gcgaaaccga cgcgacaatc gaggccaata aaaataaagc tgcttccgat
     8281 cttgctggtg ttaaagctga attgtcggat accatcaacg ctaataaaaa tgcggctgct
     8341 gtagcaactc aggaactgga cacccgcatc aataaaaaag ttgatgatat taaatcccgt
     8401 accgatgcaa ccatcgcaag caatgacaaa gcaatcaatg acaaggttga tttgattaag
     8461 gccgagactg atcggactat cgcagcaaat cacgcctatg cggaaaatca gttaactgac
     8521 acctacaata accttactgg tgtgattgca gccaataagc aagaggcagc ggataacgtc
     8581 gcagcattaa cccgtgatgt tgaagccaaa aatacagcaa ttcatagcaa agttgacaat
     8641 aacaagtctt acactgattc cgaactggca cgtttagaaa gccgtattga tgcagccgat
     8701 ggatcgagtg atggcaagta catcaaaaag cacgtaaaca cttacactga tggttatttg
     8761 ctgtctaaga ccgcaaacta ttttgatgat ccgagtgcgc gtaatcttga ctactttggt
     8821 gcattccgca tgaatgacct ggcgggtcat attgcaatga tcttgcatgt tccgcaccca
     8881 tccggcgtta atcatgctcg tggttttgag ttcacttatg ggtctaatcc ggttcatacg
     8941 gtaagaacat acggctatga tgaattggga catttggcat attctcaccg catgtatcat
     9001 gaaggtgata aacctactcc ggctgaaatt ggcgcatatt cgaaagcaga aattgaccga
     9061 ctgttccaga agacgcttaa ttttggtatc gagggtggtt ggtttaaact aggcactttg
     9121 acaatgccac aacaacacgg tcgcacggct aagatccgtt tggttggtgg taacggttat
     9181 aacgtaggac ataatggaca agccaatatt atcgaattgg tgattcgatg cggcaacaat
     9241 agcccgaaag gtgttgtatt caatgcgtat tatactattt ggcattatga acaacatttt
     9301 tgcgctattc caaccgatgg cgacaattac gatctttacg cttactacgg tgcacataca
     9361 ggatttgttt tagcagaata tcaagtttct tccggtggtg tttctttaaa cctgttggat
     9421 actcctgaat atctgggtgg tgaaaaacct gttgcagacg aaatttttga tgctcttaac
     9481 atttcatcgt tcaataactt tagcaaccgt ggaacgttaa attttgctgg aaaccatcaa
     9541 ggccaatatg acattgagca tttgaacgtg caacaaacta acagcaaaaa gatgttgcgt
     9601 cgcttccgta gttctggtcc tgccactatc tggcatgaga cagttgatga taaccagtat
     9661 cgtcttgcta ctggatacga agatacaaat caggaattat tgctgacagc aacatccggg
     9721 ttacatgtca agaaattaac attggatggc ggtgtcactg gaaattctgg tattgatatt
     9781 cgtcgaggac caaacgaatc aagccatttt aattttatgg attatcgcac tggtcaagat
     9841 gttcgtaatg gctggtttgg cttcggtgat ggtcagacca aagattttat ttggtggaac
     9901 gataacggtc aaaactcgat aaacctgatt gaagatggtg aattgcatat tactggcggt
     9961 aaaggccaga aaattgtaat gaatagcgaa gttgcattat ctgaaaatgc tcgtttggct
    10021 gtcaaaggcg gtaactatgg cttaattttt agaaatgatg gatctgactt ccatattctc
    10081 accacaggtc tcggtgattc ctttggaccg tggaatactc gcagaccatt cagctataat
    10141 tttgaggacg gtggactata tttaggtggc actgaaactt ctcgttgttt gcatctggga
    10201 attgatggta gcactcgtct tgaggacaat cttttcttta aagctggttc tcgtcaatct
    10261 atggactata ttgaaatgat ccattggggt aacagtaata tcgctcgtga caacgttttg
    10321 agtatgaaag actcgaaagg attccttgct gaatttgaac gcgtgggtgg gactaacggc
    10381 gttaaaacca gattctttgg tgaaaccttc actgacggta cattatacct aaatcagatg
    10441 aataatagct ctgaacgatt ctctatcaac aattggggaa attcagaagt tggtcgctcg
    10501 gcagtaatgg aaatcggtga ttccaaaggt tatcacttct atactgaacg caggacagat
    10561 aacagtttgg cgtttgatgt tgctggcgct tttactgtgc atggacctaa cggaataacc
    10621 atcaaaaact cagcgggtgc acgccatatc tggtttagag atgatagcga tacagaaaag
    10681 gctgttatct gggctacaga tgatggtcgt ttacatatac gaaataataa tgggggttca
    10741 tttagtcatc acttccaggg tgcaatgatt aaactggaag ggcgtgttcc ttatgccgca
    10801 gatcaaggac ttatccgtgg tgaagttgat ggtggtgcat atgttgcatg gagagaccgc
    10861 cctgctggtt tgttggttga ctgccagaaa agtattgaca gtgctcatgc tatttggaaa
    10921 gcggttgatt ggggtcgtca atatatcgca gcaatggatg tgcattgtcc cggtgatggt
    10981 aataatactg cggcagcggt tcttcatgtt cagggtgctg attatcaatt ccatgcaagt
    11041 ggagaatttc atgccactgg taacggaaac tttaacgatg tttatattcg ttccgatcgt
    11101 cgtctgaaaa ttaatgttga agattacgaa gaaaatgcgg tggataaggt aaataaactc
    11161 aaagttaaaa cctacgataa agttaaatct cttaatgacc gagaagttat aggtcatgag
    11221 attggtatta tcgcacagga tttacaagag gtattaccgg aagctgttaa aaccgcaaaa
    11281 attggtggtc ttgataaccc agaagaaatt ttaacaattt caaactccgc agtgaacgcg
    11341 cttttaatta aagcggtcca agaaatgagc gaagaaaata aacttcttcg tgaacgtctt
    11401 gctgctattg aggctaaatt atttggttaa ttaaaatagc ggggagaaat ccccgctaag
    11461 gattcttata tgggatattg ggtaagtagt gaaattaata ctaaaatcga tgctttaaaa
    11521 tggcatgtat ttgatattcc taacggctat ggtactgctc gtgctggtgt cgctgtaatg
    11581 cgtcataatg ctggtggtgg gtggtctgat gcacatatta acgtagatgg tttgaccgtt
    11641 gctcgtcgtc gtggtgggac caattgggga tctactataa attcgtttgt tcctgctggc
    11701 tgcaccgtct cctttggttt agggggtgaa ggtggtgttg aatattttaa attcatggag
    11761 ttttaattat ggctggatgg ataaatcaaa gaatgtcaaa cgctattagt atttgggcta
    11821 atggtggata ttttgatatc cccaacggct gggttactga ttcatgcgga attgtctttg
    11881 ctcatatgga agccattaac ggtgctggtg atcttgattc tgaattggtt gtaaatggat
    11941 tgattgaatc aggtcatcat gctggtgatg ctgggagttg gggcgctagt tctttagttg
    12001 gcgctggtgc taccgttagc tttacattgg gtaaaggtag tttgcattat ttcaaattca
    12061 ggagaatgca ctaatggcat tttgggtaga tggcgctttg gggaataggg cgggtaaagc
    12121 aaatcaagga tggtttgata ttcctaacgg ttggacagcc gacgctttcg ggactatttc
    12181 attctgtgct cattcttccg gtggtggcgg cggtgactct gaattgttta ttcatggttt
    12241 ttgtgtttct ggaagccata agggtcatga ttggggacag atccattctt gtattgcacc
    12301 gcaaggggcg gtaattaccg ttagcacaaa tagggggatc gctcatcttc gttatagacg
    12361 tttgagcaac cgttaataaa tagaattacg ggtatgtacc ctattgttta aaatagaagg
    12421 aaaagaataa tgactactaa atttgatatt ttctctggtg gcctcgttgg tctgtttctg
    12481 caagaagacg taaacaataa agaccttgat gctgaaacct atctggaaat cccagaatgc
    12541 gcagcgttcc cggaaactgg tgtagaacgt tctactatcg atgttcctaa cttctctggt
    12601 gcttataacc gtaagctggt tggtcgtatg tctgtaccgg atatcgaact ttcgatcaac
    12661 tacatgccgg gtaacgaagt acacgaaaaa ctgattaaag caaccgaaga cggtaagcgc
    12721 gtacagctta aaattgctta ctatgtcgat gcaactaaga aatctggtat ccagatcgct
    12781 tacaatggct tcctgtctaa aactaccatg actggtggtg aggatgctgt agtaggccgt
    12841 accttcacct ttgtagtaga tggtgctccg gttaaacaag ctgttttcca gaaagtcgtc
    12901 taatattatc tagccatcct tcggggtggc ttttttgttt ccaattctaa ataaacatat
    12961 cgaattaact taagaggaaa caagaatgaa tattaatgaa atgctgaaag ctctttctcc
    13021 gaaacgtgaa tctctgacca tcggtggatt tactttctat gctcgcccta tgtcagtaaa
    13081 agaatttaat gaacatgttt tcaataccga taaagaagac cgtgatgaac gttctattct
    13141 tcgttgtatt gaagatgaag atggtaagcc agtatttgaa tctatggaac aagttaaagc
    13201 actgtatact aacgtccgca gtgaattaat cggtttagtt gctcaagcgt cattgatgca
    13261 agatccggca gtaattgaaa acgaggtaaa gtaaacccgc ttctgaattt ctattttcgg
    13321 caaatgatga gatcggggct tagtaaagat gaaatggata atatgccaat cactctgttt
    13381 tggaaattat acattttcga cacctactta gaaccacaaa gccccgcgtt tcatgatatg
    13441 cagaatgcga tgttgcaata ttccatgtat atgacttcgc aaggaatgac acgcgaaact
    13501 gcacgtaaac tcaagcctag ccaattccaa ttaattagag aagaaaaact ctttaaaact
    13561 aaagaagaac tggaagaact tgcacgcaag aaagaagaag aacgtaaagc ggcaatgctg
    13621 aacatgtttg atccatcttt gcttgagaaa ctcagaagcg gttaaagggg taatttatga
    13681 caagacatat agtaacaata gaaggggata ataaaggtct aaggagaagt accaatgaag
    13741 ccgccgacct tctcgatagt ttgtctgaaa aggcaagtaa tattgatttt ggtggtggct
    13801 tatctggtct gactggatct cttcgtggga tcgctggctc tgctggtttg gctgctggtg
    13861 gtatcggctt agttgcgacc gcagtggttg cagccgctaa agctggcgcg gaatacgtta
    13921 aacaatattc agaagtatct aaggcgaccg gactctcgat tgaatccctt caaagactgg
    13981 aaaaggaatt ttctggcact ggcctaacag ttgaaaaatt cggtgatatc aacaaagaca
    14041 ccttagataa gatgggtgat gcgtgggcta atggtggtgg tattgccgat gacttagaat
    14101 cggttggtct taagttagaa aactatgctc acttcatgac agatccgcaa ggtggtatga
    14161 aagcggcgat ccaagtgttc tatgacatga agaaagccgg aaaatcaatg gctgaaatca
    14221 agttcatgat ggaatcttta gccagtgatt caagccatat gaccagccag cttgagaaat
    14281 ataatactgc tcaagaggcg atgatcgcta ttcagaatca atctgttaac gtcaccgaag
    14341 aaaacgctaa aaaatatgat aaattttctc agaatattaa taagctggaa aataacctga
    14401 aaggtgtcgg catgaccatt actggtcctt tggttgatag cttaaactgg ctatttgact
    14461 ggtttaatat tgattgggaa aagagttctc tattcagggc attagatcga ctgaataaag
    14521 aaggcaagac cgcaactggt ggtattctta acaccaacca taaagacgct caaaagatta
    14581 ttgacaaata caataaagaa aagcgttgga ataatctggc agattgggaa aaggccgcga
    14641 tccgtggtgc tggtgtcgat cctcgtactg ctggctttga tgttgaagga tttaagaaac
    14701 gttttggtga ttcttataag aaaaatggct ctctgattgt tgtcgataac ggggaacatc
    14761 taacccgcaa ggcagatcct aaccgtgatt tgactatccc gactacacca actcgaccag
    14821 cttcattggg taagtctggc aatgagaaga aagccgaaga agaagcacgc aagaaagcgg
    14881 aagaggcagc taaaaaggct aaggaagccg cagaaaaagc acagaaagaa cgtgaggatg
    14941 caatcaagcg tctgaatgca cttgatgtta aattgcaagg gcaagtcgcc gcgtctattg
    15001 cttctcaaaa caagcaatta gaatccagtt tgaaagattt ggacactgct ttagatctgg
    15061 gcttaatctc tcagcaagac gcagccgcga aacgccaatc cttaatcgat cagaatactg
    15121 aaaacgtata caagatgatt ttaggcgctg atccggttga tgctttgaat gccttaacac
    15181 aattgcaaca aatcagggac aacgagttag aaagccataa acggttactt gatggtaagg
    15241 ctatctccta cgaagaatat atgcgtcgtg tgaatgatac cgaacaaagt tattcacaga
    15301 ttgaagattc tttgcatgga atggatggtt ttaaaaccaa tcagttgacc aatagcttag
    15361 actatcagga ttcaaataac ccgtttgcta aatttaatgc aatcgataaa gagaaatcgg
    15421 aagcggaaca agattataaa accgataaac tcaagattga tgggatcacc gatccggcta
    15481 aacggatgga agcattagaa aaactcaatg aaaaccatca aaaacgaatg gctgcaattg
    15541 agaagaaata tgctgatgct cgccagtcaa tagccgatga tatgtatggc ggttttgctg
    15601 ctgcaatgac tctcttcggg caggaaaaca ctaaagctat gcagatggct ttcactgctc
    15661 ataaagcatt ctccattgga caagcgacgg tgaacatgtg gacggctgct accgatgcat
    15721 ggaacgatcc gaccaacgta accacaggtc aaaaaatcgc tgctgctgca ttggctgttt
    15781 ctcagaacat ggggagcatc gcaaacatca agtctactaa cgttagcggt atggctcatg
    15841 atggtatcga taacatccct cgtgagggta catggttgct tgataagggt gaacgagtag
    15901 ttgatcagcg tactaacggt gatttgaaag acttcttatc tgctcaaaaa tcaggcggtg
    15961 gtaactctca gccgattgaa gttaatgcgc ctttgaacat taacggcaac gttaatagct
    16021 cagacaagat ggtcatggat gctatcaaac gtcacgctaa gttagttgct caggcggtag
    16081 aagacgcaca gcgccgtaag atgtaattaa aagcccccat agtgataaat aatcataaaa
    16141 ctatgggggc ttttctatgt tcaaatcaaa gaatattaaa atcacagatt ttactcttaa
    16201 atcaaaacag cctttcttta aggcgcaatc tatctccggt aagttccagc gtcgctttac
    16261 tggcatccat ttttacgaag cagaatttac cgcgaatttc atggctcagg atattaacga
    16321 agtaaaagaa tttgtagcac gtcacctttt tggtcgtcct ttcagtgtgc cactgtctta
    16381 cttttcaaaa tatacaggtg atgtacgcca gatggttacg gctgctgctg gtactgctcg
    16441 cggtgggcgt aaggtgagaa tctccaactt caccggaaca ctgaaagcgg gaactatcat
    16501 ccagtttgag aaccacaaga aaatctacac gatcaccgaa gacgtgaaat caggtggtga
    16561 aatgaaactc ttccctaact tgcgtcaaaa cgtcctggcg ggtgaggtga tcaagtatca
    16621 aaacgtagaa ggtgaatttg ttctcaaaac tgaaaatatc gattggaaga tcgcccagat
    16681 tggcaagatg aaattcgaat tagtggagaa tgtataatgg caactattca ggaatcattc
    16741 agcaaactat gcactaatct ggacttcatc gaggtctaca acgaccagac aggtcagaat
    16801 gtgtctagat tgacgctacc gcagcttttc tccactggat cgatgtttca cattatcgaa
    16861 gtgataacag cgtcagggga cgttctacgg cttacagatg ggtatttcga tttggactat
    16921 aacggtttta cgtatctcgc aacgggtgat tttcttcaaa tctcatcgaa taccgaagag
    16981 aaggagatca acaacaacgg gatcaacgta actgtttcta acgttcgcga agaatacatt
    17041 accctgattc gaaacaagca attcgataaa tcagatgtga aaatcgagat ggttttcctt
    17101 aaccctaaca cgggcaaggt tgaaactact taccctgttt tccgtggggt agtcgattcc
    17161 atcggtatta acatcgaaca tgaagatgat gagtgtaaaa acgaatcaga atttcagctt
    17221 aatagtatct gggaagttct agataaaaac gctcgaagtc atgcctccga tggtatccac
    17281 cgatcctatg ccggaaacga gaacgatcta ttcttctctc gtgccgggaa gtggcaatca
    17341 gaatcaaaat ggtttagctc taaaaagtaa taccgaaatt aaatggcaca ctaaagccct
    17401 caataaatat aggagagatt ctttattttt gagggctttc ctatatgcaa aaattccatt
    17461 acgtttatca caccactatt aaatccaatc gctccggtaa aatctatcat tacgtcggaa
    17521 aaagaacaac cacaaatcta gacgatggat atgtggggtc gggtaaagtt attaaaatga
    17581 tgaagcgtaa aaataaagaa tcagaaaccc cgatatatga atttgaagtt gttcgctcaa
    17641 aattgttcga tactgccgag gaagcgtttg aatgtgaaat tctgttggtg gaggcagcac
    17701 gcgaaaagtg gggtcgtggt gtatgcctca atcttgctcc tggtggtgtc ggtggctttg
    17761 atggtggtga acatcaccca atgtacggca agaaacacaa accagaaaca atagagaagt
    17821 ttagaaaaaa caatgcaggg gaaggaaacc cgatgtttgg tcgagtaagc ccaaaacgag
    17881 gaaggaagat aactaatcca gaaacattag ctaaaatgtc ggctgcaatg aaaggcgtta
    17941 ataatcctcg ttatggtgcg atagtcaccg aagaaacccg gttaagaatt agcaaggctt
    18001 tatctggtcg aaagattacc gatcctgtag aaaaggctaa ccgaactaaa gcaattagag
    18061 aatcgaaagg tcaaggctgc tggcaacatt acgatgaaat ccgtcgtgtg tggattgaaa
    18121 acggtaaacc cggcgcggtt aaattgagtc aaatcatggt tagcttaggc catccaaaat
    18181 acaaccttcg tagaatgtac gaagacttca agaaataatc ccttctccct aagcctagta
    18241 aataacagca tggaggtatt caacatgcta aaaactaggc ttatcaccga ttatatcaat
    18301 tctttaatag gtcaggagtt cgttcagggc gagaatgatt gcaatctcat tgcatgtaag
    18361 atcatcgata ttctcgctgg tactgaccta tataattctc tttataaaaa atattcaact
    18421 aaagaagaag gcttgaaaat ctgcaaagaa ttaagcgggt attcaaatat ccttcaacca
    18481 attaagaaac atttcaaatt agtcactgat gacttacagg acggcgactt actggtcaca
    18541 gcccacaaat tagggaaccg caaatattat tccgtagttc ctcattattc cggttatggc
    18601 ctcgttgaag aagatggtat ctggatgacc attcctgttt cagacattga ctatgaacaa
    18661 gtttatagat tcgggggtga ataatgggat ttgaaatatt ggtaggcgcg gtgatcgctg
    18721 gtgcttcggc ggggatggcg gcagccgcaa cattttctgt tatgaccgca gtcgctatcg
    18781 gtatggctgc tggtgcaatg accttgatcg cttccactgt aggcgcacca aaaacaccga
    18841 aagtacaaag cccagataat gcggtgacac taggaacatc aaacgatcct aagacagtat
    18901 tacccgttct tttcggtact acccgaactg gtgcaatctg cgtttacaaa gcaatttcac
    18961 agcgtgaaaa taacaaactg gtacaaattt ttgctattgc cgaaggtgag atcgatcact
    19021 ttaaagcact gcacatcgat aataaaaacg ttctcattag tcagaatatg acaattcgtg
    19081 atggcattct cgataaagga aacattaaag acgaatatcg caaagtgtta gaagtcgagt
    19141 ttcgcacggg taaaaatcct aacactgcat tagatcttgc taaacatcat ttgggcgctg
    19201 attgggatga tcggtatcag ggtaacggca ttgcgactat gtgtattgtt ttacgtcgtg
    19261 atgacaaatc tttggctgct ggtgttgata ttctccagcc taatagccag gtagcggtcg
    19321 atgttatggg cttaaaaatc cgtaacctcg aaaccaatgc tattgaggct agcactaacg
    19381 gagtggatca gattttccac tacctaacaa atgaaaaata tggcttatcc gtaccaattg
    19441 aaaacattaa cgttgattca ttcctgaaag tacgtaagca agtacgccag atggatttgc
    19501 attcaaacgg tgcatgtgat ccgaacgcca gctttaaaga gaacttaact agccttatgc
    19561 agactttcgg cggggtaatg ttcgaatcct ttggacgtat tacgctgaaa ctggatgctc
    19621 ctgatattgt taagcatacc ttcaatgaag acaatatcat gatgggtaag gtatcactga
    19681 aaacaggtgg cactaacggt tatttcaata ccattaacgc gatgtatcaa gaaccatcaa
    19741 ttgactattc agagcaaatg ctacgttatc cggctgatgc tgaaaacgat gctactgttc
    19801 gcgcagatgg tcgaattatt gctaaggatg tagaatatcg tttcgttaag tctaaagatc
    19861 agattgataa actcgcgagc attgagcgaa ataaatctcg tatcactcag gttatcagct
    19921 ttatgactac tgacgcattc actgccgaag tttgggacgt tatcagcgta acctatgatg
    19981 aattaaaact gaataattca ctttggcgca ttactgcaat tgatcgctcg attgattctg
    20041 gtattgctgg gatgatgact atcaccgcca cagaatataa ttctcaggtt tatactgacc
    20101 tgaactatgc ggcgactcca gacaatagac ctactggttt accggattca atgacagtac
    20161 agaaaccgac taatttcaga attaaggcaa ctggtgagac aatccacggt aagaacgtta
    20221 ctttaacatg ggatgcaccg gaagatttca atcgctacgg tttccagatt gattatcgtg
    20281 tgagtggatc acctaactgg attaagctgg gacagacttc acagcaaatt ttcaatatca
    20341 atgcactggc aaaagatcgc tcctacgatt accgcgtttg tgctttcggt attattgctc
    20401 gttccgaatg ggtggaactg gttaaccaga atccagaagt tacctatgaa ctgcctacgc
    20461 cagtgatccg catcaaaaat cagggtagca caccaggaac ttttgaaggc aatgatctga
    20521 ttatcgaatg ggaaaatcag caacaattag atgtgatgat caacggtgaa actaacaagt
    20581 ttagtgacct gtttgaagcc tacattatca aggtaactaa caaggctggt aagtctattc
    20641 agtaccgtac ccgcgatcct gaatcatgga cctatacgct tgatatgaac cagtttaacg
    20701 gcctttcccg tgaattgacg gtagaagtat cagtcaaggg ctataacaac tcagagagcg
    20761 ccccagcgcg tttagtggct attaacccac agcataagcc aatgaaaggt tttagtgcgc
    20821 gtggtggctt taatactgcg tttgttagct gggcggatga cgtagaacat gactatgcag
    20881 ggtcaatcat ccagtatgca accgataata ctttctccga tgcaagggca gtgactacca
    20941 atagcgttag tcatacttcc tttgatttgg ctgacggtga ttattatatc cgtggtgctc
    21001 actacgatat tttcggtatg gatgatgctg tttggtctga accgtatttc atgcaaatga
    21061 aatctaccat tagctgggac gatcaggaca aagaagcact ggaagacctg attggtttac
    21121 aagatcgctt agatgaaacc atcgcggatg ctattgctca agctggcgct aatgccgatg
    21181 ctaaaatcga tgcaatgcat aagcaaatca ctaccgaaac agggcagacg gtccaagcct
    21241 cagccgatac ccttaagagt ctgattgcta ccagtgagaa agcaagctct actaagattg
    21301 atcaggttaa agctgaactg aaaggcgata tcaccaatga agttagcgca tctgctacta
    21361 ccctgaaaca agcaattgct accagtgagg cagcaagcgc aagtaagatc gaccaagttc
    21421 gggtagaaat ggatggcaag attgctggtg tgaatcagga agcggacgtt aagatcgatg
    21481 ctttgaaggg aaccattaac agcaaatata atctggcagt taatgcagat ggtcgcgtgg
    21541 ctggtattca tatgagcgca accaatgatc cggcagaacc gacgagaatc atctttacgg
    21601 ctgataaaat cgcggtagct ccacagaacg gatcggaagt atgcccgttt ggaatcgaag
    21661 ataacaaagt ttatctcgat aatgcgatga ttcgtaatgc tgcaattggc acagcccaga
    21721 ttagtgatgc tagtatcacc acggctaaga tcggcacagc ccagattaac ggcgctcaca
    21781 tccaacacgc acagatcgga acaggtcata ttattgatgg ctctatagat aatgctaaaa
    21841 ttggtaacta tatccagtct tataactgga atggtaacga tggatggtat atcgggaagg
    21901 atggcacttg tcattttaga cacgctaaca ttcgtggtca tttggtggct gattccggcg
    21961 aaatgaacaa cgtaacgatt aattcaagtt gccgcatttt gggtatgctt gatgctaatc
    22021 aggtgcgtgg tgacttcgtg aaagtggttg gtaggagttt cccgcatcgc caaccaaact
    22081 ggcctgatgg tgcaatcact gtaagggtcg aagatgatca tccattcgat cgccaaatag
    22141 tgattccagc gattcatcat aacgggtctg ttcgtcgtgg cgaaagtagc gatcattggg
    22201 atacttgccg actaatcgtt aagaaaaatg gtggtgttct atatgatcgc actgcaactt
    22261 ctggctgtat gtacagtgga gttattgaca tgccagccgg acacggggca gtaacaatga
    22321 cttttgaagt ttactgtaag ggcgttaaca actataaccc aagtgcatat atcagcgatc
    22381 taacggtaat ggtaactaaa aaagccacta ccggtattac tgtttcttaa aatttaattt
    22441 taataattaa tcccgcttaa ttcttttgag
//
LOCUS       DAPGRN010000077        22436 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPGRN010000077 DAPGRN010000000
VERSION     DAPGRN010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.077"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            183..1082
                     /gene="yegS"
                     /locus_tag="RHW33_003102"
     CDS             183..1082
                     /gene="yegS"
                     /locus_tag="RHW33_003102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV1646582.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDNIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1164..1937)
                     /locus_tag="RHW33_003103"
     CDS             complement(1164..1937)
                     /locus_tag="RHW33_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV1646583.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2043..3083)
                     /gene="gatD"
                     /locus_tag="RHW33_003104"
     CDS             complement(2043..3083)
                     /gene="gatD"
                     /locus_tag="RHW33_003104"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV1646584.1"
                     /translation="MKSVVNDTDGIVRVAESVFPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLSPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLVLVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3131..4486)
                     /locus_tag="RHW33_003105"
     CDS             complement(3131..4486)
                     /locus_tag="RHW33_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HDV1646585.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4490..4774)
                     /gene="gatB"
                     /locus_tag="RHW33_003106"
     CDS             complement(4490..4774)
                     /gene="gatB"
                     /locus_tag="RHW33_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV1646586.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVERSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(4805..5257)
                     /gene="gatA"
                     /locus_tag="RHW33_003107"
     CDS             complement(4805..5257)
                     /gene="gatA"
                     /locus_tag="RHW33_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV1646587.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5267..6529)
                     /gene="gatZ"
                     /locus_tag="RHW33_003108"
     CDS             complement(5267..6529)
                     /gene="gatZ"
                     /locus_tag="RHW33_003108"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV1646588.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYRELVRDHFA
                     ILKVGPALTFALREAVFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6558..7412)
                     /gene="gatY"
                     /locus_tag="RHW33_003109"
     CDS             complement(6558..7412)
                     /gene="gatY"
                     /locus_tag="RHW33_003109"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV1646589.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7709..8761)
                     /gene="fbaB"
                     /locus_tag="RHW33_003110"
     CDS             complement(7709..8761)
                     /gene="fbaB"
                     /locus_tag="RHW33_003110"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV1646590.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9018..10295
                     /locus_tag="RHW33_003111"
     CDS             9018..10295
                     /locus_tag="RHW33_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1646591.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFTGAALMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10292..11296
                     /locus_tag="RHW33_003112"
     CDS             10292..11296
                     /locus_tag="RHW33_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV1646592.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11293..12258
                     /locus_tag="RHW33_003113"
     CDS             11293..12258
                     /locus_tag="RHW33_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1646593.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12232..12978)
                     /locus_tag="RHW33_003114"
     CDS             complement(12232..12978)
                     /locus_tag="RHW33_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1646594.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQNIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13030..13848)
                     /locus_tag="RHW33_003115"
     CDS             complement(13030..13848)
                     /locus_tag="RHW33_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV1646595.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(13913..14713)
                     /gene="thiD"
                     /locus_tag="RHW33_003116"
     CDS             complement(13913..14713)
                     /gene="thiD"
                     /locus_tag="RHW33_003116"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV1646596.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14710..15498)
                     /gene="thiM"
                     /locus_tag="RHW33_003117"
     CDS             complement(14710..15498)
                     /gene="thiM"
                     /locus_tag="RHW33_003117"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV1646597.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGRRTVGIHGGDPLMAKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQELQA"
     regulatory      complement(15533..15629)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15721..15993)
                     /gene="rcnR"
                     /locus_tag="RHW33_003118"
     CDS             complement(15721..15993)
                     /gene="rcnR"
                     /locus_tag="RHW33_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV1646598.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16114..16971
                     /gene="rcnA"
                     /locus_tag="RHW33_003119"
     CDS             16114..16971
                     /gene="rcnA"
                     /locus_tag="RHW33_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV1646599.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMYGHDHHHHDHEHHQDHDHDHDHDHDHEHHHHHEHGDNEEY
                     QDAHARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGA
                     TLVVSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMG
                     VHGFMGIMR"
     gene            17189..17527
                     /gene="rcnB"
                     /locus_tag="RHW33_003120"
     CDS             17189..17527
                     /gene="rcnB"
                     /locus_tag="RHW33_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV1646600.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNASGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17609..18643)
                     /locus_tag="RHW33_003121"
     CDS             complement(17609..18643)
                     /locus_tag="RHW33_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV1646601.1"
                     /translation="MKIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLLSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(18659..21139)
                     /locus_tag="RHW33_003122"
     CDS             complement(18659..21139)
                     /locus_tag="RHW33_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1646602.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGAWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSLSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21155..21829)
                     /locus_tag="RHW33_003123"
     CDS             complement(21155..21829)
                     /locus_tag="RHW33_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1646603.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(21910..>22436)
                     /locus_tag="RHW33_003124"
     CDS             complement(21910..>22436)
                     /locus_tag="RHW33_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1646604.1"
                     /translation="AVAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSV
                     SINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDK
                     AIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
BASE COUNT         5679 a         5716 c         5168 g         5873 t
ORIGIN      
        1 ttgaaattta tcggtggctg ccgttagtat tcgctgtccc cattgcaagc tcctggtggt
       61 aaccactgaa tcccccatac ttgaactgac tttttattct ccgactttca tcctgttctg
      121 actccacctt ttcttttctg ctctacacta tctacagacc aatcataaag gcacatacga
      181 tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact gacaatttac
      241 cattgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat gtgcgggtca
      301 cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg ggcgtcgcaa
      361 cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg ttgattcagt
      421 gtgaggggga taacataccc gcgctgggaa ttttgccatt aggaaccgcc aatgattttg
      481 ccaccagtgt agggattcct gaggcactgg ataaggcact gaaactggca attgccggta
      541 acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt aatatggcga
      601 caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa gccgcgctgg
      661 gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa ccggaccgtt
      721 gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt ggtattggta
      781 acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa cgcgttaatt aacgatggct
      841 tgctgcaact gcgcattttt accggcgatg aaattctgcc agctctcgta tcaaccttaa
      901 aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt gatatacaag
      961 ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa aactttcata
     1021 ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca ttattgcgtt
     1081 aatcaaacta ttctctcttg aaccagggag aatccgtaat gccggatgcg gtgtaaacac
     1141 cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt cttctgcaat
     1201 gccagtttat ccacctctga cacagcatca tcggtaacaa taatgtcgat tttttcgatt
     1261 ggcagtacct gattaaaacc gcgacggtta aatttcgatg aatcgagaac ggcaatcact
     1321 ttattagcgg cggtgaccat cgccccgcta atcgaataac cttcattaaa ggtcgtaata
     1381 ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt aatatcctgc
     1441 aatgaacgct cggcaatgga accatgcatc gagcgcgttt tatggcggac ggtgccacca
     1501 cagacgacca gagtaatatc tttattttcg gaaagggcaa acgctgccgg gagactgttg
     1561 gtaatcaccg tgatattttt ggcggtcatt aatccttcgg cgataagcat tgtcgtactt
     1621 ccgctgtcga gaataacggt catcccttcg tggatcattt tgactgccgc ctgcgctatt
     1681 ctgttttttg gcgcgctggc gagctgaaat cgctctttaa acccgacttc ctgggtctcg
     1741 gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt aaccacgcct
     1801 ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc aaatactccc
     1861 gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat cttattcctt
     1921 cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt aaacgaaggg
     1981 ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc tggcccgcgg
     2041 tttcagggaa tgagcaacac tttgcccggc atagcattac gagcgatgtc acgcaccgcc
     2101 tgggcgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct taacttacgt
     2161 tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct ggagtagttc
     2221 atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt tgccgatgtt
     2281 aaatgcagat cctgatgcaa cgtgcccacc agcaccagtt gggcatgagg accggcaatc
     2341 tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag ctgattaaag
     2401 cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca tttcgctact gttaaatgtt
     2461 tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc gatagccgtc
     2521 acactctttg ctcccagcgc gacagcgcac tgaatggcca gcaggccaat ggttccggca
     2581 ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc atgcaggcca
     2641 acggtaatcg gttcaataaa agccccatcc tcaataggca tatccgtggg tagagcaaag
     2701 acatttttcc gcttaacgac aatatattcg gcaaatccac catcacgccg tgagccaata
     2761 aaatcatatt ttgcgcactg ggaataaaac cctttcagac actctggaca agtaaaacag
     2821 ggtgataacg gcacacaggc aaccgcatcg ccaggatgta aatcatcaac accggatccc
     2881 accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata atgtgcacca
     2941 tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat ttttacccga
     3001 acctcatcct gatgtttaat ttcaggaaag acgctttctg caacgcgcac gataccatca
     3061 gtatcattca ccactgattt cataaaaact cctgattgtt aagggggata acctccccct
     3121 caggtaaaaa ttattctgcg agaacgactt tctcttgttt aataaagcca cgcgctctac
     3181 gccaggtcat gaaaataccg gtaagataaa ttgcgccgat aataatgaaa ccgggaatat
     3241 tttgcgggga gaaaacctga atcagtaacc aggtaatggg agaaccgccc tgatccattg
     3301 aagccaccat acccccggct tttaacgccc cagcattagc cgccagttgg gtgtgcaaac
     3361 caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc gagattaagg
     3421 tgcggaacag atttccacga tgcaccgcga ccgccatcgc cacaaagaag ccgatagtgg
     3481 caagatcgcc aaacggcagc acctgattac ccggcacaca aacagcaatt aaaatggtga
     3541 gcgggataaa aatcaggctt gccgatacca ccgccgtatg ccccagcagt aatgcgggat
     3601 ccaggccaat caggaactcc tgaccgccga acttcgcctg taaacgacta cgggcctgct
     3661 tagcgatggg cgttaaacca tccatgatgg gtttaatcac ccgtggcatc agcagcatca
     3721 ccgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc gcgaggatgc
     3781 cgataatcag ccccatcaca aaacccacgg tgacaggctc gccaaacgga ccaaatttgc
     3841 gctgaatatc gtcggcgcta aatttaatac ggtttacgcc tgggattttc tcgataatgg
     3901 catcgaccag caccgcaatc ggccccatat acgccgacgt accgtgcgga atagcaatgc
     3961 cttccagctc aaagaaattt cgggtatcgc gggcaaacca gtcgccgagc ttataaacaa
     4021 acgccgcgtg aattaccaca cccgccatcc ctatcatcca tgaaccggtt gccagatgca
     4081 gcaacgcgcc ggtgaaggtc atatgccaga tattccagat atcaacattt accacccgcg
     4141 tcatacgggt cagtaacatc gccacgttaa ccagaatcgc aatcggaatc gccaccagcg
     4201 caatttgcga cgcccaggtc attggtgaag agcccggcca gccaacatcg accacatgca
     4261 gattcaggtc gaaattttcc gccatcgctt tcgccgccgg gccaatggaa tccagcatta
     4321 agccaatcac aaggccaatg ccaacaaagc caatcccgat atgcagaccc gctttaaagc
     4381 aatcgcctgc cttcatgcct aatattttag aaaaaataat gatgacaatc ggcagcatca
     4441 ccgtagggcc gaggtcgaga atataacgca tgacttctga aaacataggt cacccctgta
     4501 agatagtcag aattttattt tgtaatgctt cgataccgac accagaaaca aaaggcatgc
     4561 cgtgaactaa tggaatatcg ccaaaactac gctccacttt ggcagtggtg catatcaaat
     4621 gcacgccatc catataggtt tctatttcat taacccgaca ctggattaat tcaacaggaa
     4681 tattatgact ctgacacaac tctttaattt cttccgccgc catcgtagag gtcgcaaccg
     4741 cgcctccgca agcgacaata atcttgcgtt tcataaatag tcctttttat tgttacagag
     4801 ggatttattc gtctgaatct aaaacatact ttgtgaagta ttcctttaac tgagtttcag
     4861 gaagagtgat tagtgtctca acgatatcgg gctgttgtaa cttgccaaat aaacagcgta
     4921 aaagtttcaa ttgctgctgc ggattttcca caatcaacgc aataaccaac gataccgcca
     4981 cgtcgttatc atcatccgct tgctgaaaat gaactttatt tgttggcctt aacagataaa
     5041 tggctgacga cttagcatga atcgcctcac aatgcggtat tgcaatggcg tgctgctcaa
     5101 gcattatccc ggtagggaat tctgcttctc tggcaattaa tgcctgtggc caggtatcat
     5161 gaaccacacc tttggcgagc atctcattac cgatatgggt taaaacttcg ctacgatcga
     5221 caaaagaaat tccgctacga acaaacaggt tagtcatata ccgtccttat tccgcacagc
     5281 cgtagcgata ggcgcgcaaa acatcataaa ttttatccat aataagctga tgcggtattg
     5341 ctgataattc cccggactga atgcgttcaa attgtttagg aagatactga ctaatcatgc
     5401 ccagtggtat gtccacgcct tccagattca ccatcatcgt ttcaacgcta tttttaatcc
     5461 gactatgcgg ccagtaataa cgaatacgat ccgacaggct gtaacgaata tccagtaatg
     5521 aatcgttaaa acccgtacga taatattttt tccagtactg cggttcgtcg agcatcactt
     5581 cttcaattac cgccaggcaa ccgctgcgat tttcgggggc gataagttcc tgctcaattt
     5641 gtgccagcgc aaataccgcc tcacgtaaag caaaggttaa tgcgggaccg actttcaata
     5701 ttgcaaagtg atcgcggact aattcccgat aagccgtccg ggtctggtaa tcggtagaat
     5761 gtgcttcata aaccattcgg gtgttttcta tccattgcgc cagcgcctgc gcttcctgcg
     5821 gctgataatg gataatattg ctgtgatcaa attccacgcc cggctgcacc acgatggcaa
     5881 tcacgcgtgt taacgcctct gtcagcccac gggcaataaa ggccttttga tgcgtacgta
     5941 aagtattggc ggcatcttca acatgggtga tgtgtactga ctgaatggcg ctggcctcac
     6001 cgcccggaac cggaacttcg gtgccaatga catagctcag ttgctcacgc tggcaatctg
     6061 tcgccacact ttccgcagca aagcaaagca cagcagctcg ttccgcaacc gtttctggtg
     6121 ctaacggtat gggatccccc gcgcaggaca ttgacgcatc aagatgaatt ttactgaagc
     6181 cagcacgaac atatgccttt accagctcga cggatttttc catcgccgca tccgcatttt
     6241 cttgctgcca gcagtttggc cccagatgat cgccgccgag aataatgcgt tcgcgtgcaa
     6301 acccaacttt atcggcaatc gtaaaaacaa attcgcgaaa gtctgccggt gtcattccgg
     6361 tataaccgcc aaattgattg acctggtttg acgttgcttc aatcagcact ttgcgcgtgc
     6421 tgttgcgatc aaatgccagc gccgcttcga taaccaacgg atgggcagaa cagactgaac
     6481 atatgccgat atgttcacca gctttatgcc gggcaattaa cgttttcatg ttttttcctt
     6541 gttaaatggc aggtgcgtta tgccctgccc tcgcagccac aatcggcaat cactttgctc
     6601 accacatcgc gcattgcgga tttagccgac tgcaaataat cccggggatc ggtcgcttcg
     6661 gggtgctcgg tcaggtaatt ttttaacgcc tgcgagaagg catttttcag ctccgttgca
     6721 acgttgattt tgcatatccc cagtttgatg gtttgctgaa tatccttagt cgataatcct
     6781 gacgcgccat gcagcaccag cggtaagttc acccactggc gaatattctc cagtcgagaa
     6841 aaatcaagcg ccggtgcgct ggcatacatc ccatgagccg tgccgatggc gaccgccagg
     6901 gaatcaattc cggttgcctc ggcaaattca cgcgcctgaa cggggttggt gtacaacgca
     6961 tcggcttcat tgacttgcac atcatcttcc tggccgccaa gttgccccag ctccgcttcg
     7021 acgctgacat caaagcgatg gcaaaaatcc accacctctt tgacccgtga aatattttgc
     7081 gcaaaaggca aatgcgaggc gtcaatcatg actgagcgca cgccagaacg aaccttctga
     7141 gcgatatcgt caaatttcgt gtgatggtcg agatgaattg ccagtggatg gtgatattgc
     7201 ttcgccatcg cgctgaccag cgccaacaga ttttctgtac cagcatgagt aaatgtgcca
     7261 ggcgttccgg cgatgatgac cggcgcatgc aggttggcag cggtttctac caccacttgc
     7321 atcgtttcga gattgtgaat attgaatgcc ggaaccgcat aaccgccgcg ctgtgcgttg
     7381 ttcagcatct gctttgtcga taccacgtac attttcatat cctgtcgttt gttttcgatt
     7441 tcaaaatata atgaaatgat ttgtttttaa atgtcgagat aacgatcaca aaaacgacaa
     7501 tatgaaaatt aagccagaat gacaggcaaa aaaaacggcc tcccagggga agccgtaacg
     7561 aagtgtgcgt gtttttatgc cggatgcggc gtaaacatat gtggcagcgc tgcgtaggca
     7621 tgataagacg cttcagcgtc gcatcaggca taggttgccg gatgcggcgt aaacgcctta
     7681 tccgtcctac ggggtagtgc cgttcgtatc aggcgatagt aattttgcta tcgagataaa
     7741 cgtcctgcac ggcgttaatc agtttcacgc cgtcagccat cgatttcttg aacgccttac
     7801 gtccaagaat cagccccatt ccgccagcgc gtttgttgat aaccgcagta cgcactgcat
     7861 cgctgaggtc agtttcaccg cctgcagcac cgccggagtt tatcaacccg gcccggccca
     7921 tatagcagtt agctaactga taacgcacca gatcaatcgg gttttcgctg gttaacttgc
     7981 tgtacacgcg atcgtcggta taaccgtaat taattgcttt atagccgccg ttattttccg
     8041 ccattttttg tttgacgata tctgcaccta tggttgccgc cagatggttc gcctgaccag
     8101 tcaggtcggc ggagacatgg taatcaacgc catctttctt aaaggcggag ttacgcaaat
     8161 aggcccacag cactgtcacc atgcccagct cgtgcgcacg ttcaaaagcc gcagaaattt
     8221 cttcaatctg gcgacgtgac tcttccgagc caaaatagat agtcgcacca accgccaccg
     8281 cgcccatgtt gaaggcctgc tccacgctgg catacagcgt ttgatcgtag gtattcgggt
     8341 aacttagcgt ctcgttgtga ttaagtttga cgagaaatgg aatgcgatgc gcatagcgcc
     8401 gcgataccga cgccaacacg ccgtaagttg atgccacaca gttacagccc gcttcgatcg
     8461 ccagttcaac aatgtttttc gggtcaaagt agagcgggtt agcagcaaat gaagctccgg
     8521 cagagtgctc aacgccctgg tcaacgggca gaatagaaag atatcctgtg ccagccagac
     8581 gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc tggcgggcga ttattgtcaa
     8641 tcatcacgcg gtctacgtag tcatgtccgg ggagataaag ctggtcagaa ggaatagtca
     8701 tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag caactgcgca atatctgtca
     8761 ttactatgct cccgtaaatt ccgattggat atcggctatg gattgtcctg gcccgccttt
     8821 tgcgggcaat cataatcctg gtcgttacgg gcgagttttt ccatcttttg gatgtttttt
     8881 cagcgttttc tgttggctcg attcatcaga aaaaatgtta gcgcggtcaa gttttcaccg
     8941 caaaggtatt taaaaggtat tattaagtgg tattgtcatc gcgtacctta cattacctgt
     9001 catgaaggaa ttaaaagatg aaaacaacag caaagctgtc gttcatgatg tttgttgaat
     9061 ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg gttaagtaaa agcggtttta
     9121 gtgccggaga aattggctgg tcgtatgcct gcacagccat tgcggcgatc ctgtcgccaa
     9181 ttctggttgg ctccatcact gaccgctttt tctcggcgca gaaagtgctg gcggtattga
     9241 tgttcactgg tgcggcgctg atgtatttcg ctgcgcaaca gaccactttt gccgggttct
     9301 tcccgttact gctggcctac tcgctaacct atatgccgac cattgcgctg actaacagca
     9361 tcgcttttgc caacgtgccg gatgttgagc gtgatttccc gcgcattcgt gtgatgggca
     9421 ctatcggctg gattgcctcc ggtctggcat gtggtttctt gccgcaaatg ctgggttatg
     9481 ccgatatctc accgactaac atcccgctgc tgattaccgc cggaagttct gctctgctcg
     9541 gtgtgtttgc gtttttcctg cccgacacgc caccaaaaag caccggcaaa atggatatta
     9601 aagtcatgct cggcctggat gcgctgatcc tgctgcgcga taagaacttc ctcgtctttt
     9661 tcttctgttc attcctgttt gcgatgccac tggcgttcta ttacatcttt gccaacggtt
     9721 atctgaccga agtcggcatg aaaaatgcca ccggctggat gacgctcggc cagttctctg
     9781 aaatattctt tatgctggca ttaccgtttt tcactaaacg ctttggtatc aaaaaggtat
     9841 tattgcttgg tctggtcacc gctgcgatcc gctatggctt ctttatttac ggtagtgcgg
     9901 atgaatattt cacctacgcg ttactgttcc tcggtatttt gcttcacggc gtaagttacg
     9961 atttttacta cgttaccgct tacatctatg tcgataaaaa agcccccgtg catatgcgta
    10021 ccgccgcgca gggtctgatc acgctctgct gccagggctt cggcagtttg ctcggctatc
    10081 gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatca ggaaccggta aacggactga
    10141 ctttcaactg gtccgggatg tggactttcg gcgcggtgat gattgccatt atcgccgtgc
    10201 tgttcatgat ttttttccgc gaatccgaca acgaaattac ggctatcaag gtcgatgatc
    10261 gcgatattgc gttgacacaa ggggaagtta aatgaaaaca gaacgtattc tcggtgctct
    10321 ttatgggcag gcgttagggg atgcgatggg gatgccctcc gagctttggc cacgcagccg
    10381 cgttaaagca cactttggct ggattgaccg ttttctgcct ggaccaaagg agaataacgc
    10441 ggcctgttat tttaaccgcg ccgaattcac cgacgatacc tcgatggcgc tgtgtctggc
    10501 ggatgcgtta ctggaacgtg aaggcaagat cgatccgaat ctgattgggc gtaatattct
    10561 cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta cttggtccga cctcgaagat
    10621 tgcgcttaac gccattcgcg acggtaaacc cgttgctgaa ctggaaaaca acggcgtgac
    10681 aaacggcgca gcgatgcgcg tctcgccatt aggttgtttg cttccggcgc gcgatgttga
    10741 ttcctttatt gatgatgtgg cgctggcatc cagcccgacc cataaatccg atctggcggt
    10801 tgcaggtgcg gtagtcatcg catgggcgat ttcccgtgcc attgacggag aaagctggtc
    10861 agcgattgtt gattcattgc cttcaattgc gcgacatgca cagcaaaaac gcatcactac
    10921 cttcagcgcc tcactggcag cacgtctgga gattgcgctg aaaattgtgc gcaatgccga
    10981 cggcactgaa tccgccagcg aacagcttta ccaggtcgtt ggcgcaggta ccagcactat
    11041 tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca caaaccgacc cgaaccgttg
    11101 cgccgtcctg tgcgctaacc ttggcggcga cacagacacc atcggtgcta tggcgacggc
    11161 aatctgcggc gcgttgcatg gcgttaacgc tatcgatcct gcattaaagg cggagctgga
    11221 tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg ctggcgaaat atcgtcaaca
    11281 acgggaggcg gtatgagcgg cgctcgatta cacacgctgc tgcctgaatt aaccacgcgt
    11341 cagccggtga tggtcgtcgg cgcggcggtc attgacgtga tcgccgacgc ctacgccctc
    11401 ccctggcgtg ggtgcgatat cgaactgaaa cagcagagcg ttaacgttgg cggctgcgca
    11461 ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag cgggtaatgc cttgccgctc
    11521 ggtcagggcg tgtgggcgga gattattcgc aaccggatgg cgaaagaggg gttaatcagc
    11581 ctgatcgata acgccgaagg cgataacggc tggtgtctgg cgctggttga gccggatggc
    11641 gaacgtactt ttatgtcatt cagcggtgtg gaaaatcagt ggaatcgcca gtggctggcg
    11701 cgattaaccg ttgcgcctgg cagcctgctc tatttttccg gttatcaact ggcctcgccc
    11761 tgcggcgaat tgttagtgga atggctggaa gagctgcaag acgtgacgcc gtttatcgat
    11821 tttggcccac gtattggcga tatcccggat gcattactgg cgcggatcat ggcctgtcga
    11881 cctttagtgt cgctcaatcg tcaagaggct gagattgccg ccgaacgttt tgctttatcc
    11941 gcagagataa caacacttgg caaacaatgg caggagaaat ttgccgcgcc gttgatcgtt
    12001 cgtctcgata aagaaggcgc atggtatttc agcaacgacg cttctggctg cattccggca
    12061 tttccaacgc aagttgtcga caccattggg gcgggcgaca gtcatgccgg tggcgtactt
    12121 gccgggctgg cctctggtct gccactggcg gatgccgtat tactgggcaa tgcagtggcg
    12181 tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa cgcgcgagga actactcctc
    12241 gcacacaaaa acgtatagat cgctgcgaca gtggctaatg ctgtattcaa taggccgttg
    12301 ctgttggtca agcgccactt gcttgatcac cagcaccggt attttgctat ctaactgaat
    12361 atgtgactga aactcggcat ctggcatccg ggcgctaacg cgggaacgtg tacgttgtgg
    12421 gtaaatgttc tggctgcgga aatagtcata aagagaaatt ccgatggcat caacatcatg
    12481 aattaaatgc gccggaaccc acgattcctc aatcgatacc gcttcttcat cgacatagcg
    12541 aatacgctta agcaaaaaga catcacttcc cgcttcgacc gccagctgct gcgcgacttc
    12601 ttcgggacat ttcacgacac gcttgttaac ccatagcgta tcgggctttt tcccgcgcaa
    12661 taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag tattcgaaga tgttgttgat
    12721 ctgcgtgccg taaccacgcg aacgcgtcac cacgccctct tcttccagcg cctgcatcgc
    12781 cttgcgcacc gtaatgcgcg aaacgccggt taactgactt aaatcacgct caccgggcaa
    12841 aatattgcca tgctccagca ccccgctgcg cacggcattt tttaccgttt cggcaaactt
    12901 aatataaagc ggcgtgttat ccgccgctaa aatacgttcg ttcagttggg caatcagctg
    12961 ggtatgcgct tgttccattt gtctttttcc tgccagggag tctggtggga gtatatcact
    13021 gcttcttatt taaggcgttt ctttaatccc atcaacgaat gcctgcaact cctccaccgt
    13081 gccattaaac acattaaaat ccaccggacc attgatgcca tctacctgtc cacggtcgga
    13141 atgctgccag aagcgccagg ccatgccgtc attgtccgga cgacgttgat agtagtgagc
    13201 cacccaccac ggatattcat tgaaatagcc cgccagattc gtgtgataaa aaacggctcc
    13261 tgagtaaata atcggctttt tccccgtact tttttcgacc atttttagcc actgacttac
    13321 ccgcttgcgt aattcttttg ccgataattt tccccgttct tctacgtcca gcacggcagg
    13381 gaaatcgcct tgtgagaaat ccaccgtttg cagaaataat cgcgcctgaa caggcgctgc
    13441 taccgacggg gagaaataat gatacgcccc gcgcagcagg ccattttcgc ggcttagttg
    13501 ccagttacgc gaaaaatagg ggtccaccag cttttcgcct tccgtcgcct taataaaggc
    13561 aaattgtaag cgaataccgt tatcacgcat ttttgccacc cgctgccagt cgatccgttc
    13621 ctgccagcgc gaaacatcta ttccgtgaat ggtgtaactg gcaggaatac gaatagcgaa
    13681 agatttaacc gggcgataac cgtagaagtg gatgtagtct gtaatctgaa ctgctgtcga
    13741 atagaaaaaa ttcaccgtct gacgcggata aattgcaacg atggaaatta gcccaagagc
    13801 gcataataga gcggtgagtt tttttcgact ggtaattctt agctgcattg gtttcatccc
    13861 tgaatgtcag tgccaaaggc tgacaataac caaagcagct atagtacggt gctcaccacc
    13921 aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac ttccagcgtg tcggcctggg
    13981 ctaacgccga tgaaagccag ctttttgcct cctgtacggt gtcagcccag tttgtatggc
    14041 gcgggcgtag tgcagccaac gccgcagaga gtgtacaacc agtgccgtgg gtgtttttgg
    14101 tcataatgcg cggtgcggta aaccgttgtt caccctcgcg ggtaaacagc cagtccgggc
    14161 tttgctcatc atccagatga ccacctttca ttagcactgc gccacagccc atcgccaaca
    14221 gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc gtgtggcgcg tcgagcaagg
    14281 cagcagcttc gggcaagttt ggcgttatta atgaaacctg tggcaataat cgactgcgta
    14341 acgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct ttttgccagc ataacggtgt
    14401 cgagtaccac gttttggatc tgataacgtt gcaaccgttc tgccaccgct tcaacaatat
    14461 cggtttccgc caacataccg attttagtgg tgtcgattcg cacatcgctg aacaccgaat
    14521 cgagctgggc agcgacaaaa tcaggctcaa tgcgatacac cgactgtacg ccacgggtgt
    14581 tttgcgccac cagagcagta ataactgagc aaccataagc gccaagtgcc gagaaggttt
    14641 taagatcggc ctgaatcccc gcaccaccac tcggatcagt accggcaatc gtcagagcgt
    14701 taattcgttt catgcctgca gctcctgcgt caattgccag agcgcatcaa ggaaatgtgg
    14761 aacaaaactg cctggcccct cgcttctggc gactgcgcgt tctccggctt gtttcatcca
    14821 gtgacaggca gatgcgacat tttccagcat atcgcctggt aacgcacagc aggcagcgac
    14881 aactgccgat aatgcacagc cagttcctac cactttggcc attaacggat cgccgccgtg
    14941 aatgccaacg gtgcgacgac catcggtaac ataatccacc tcgccagtga ccacgacgat
    15001 tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc gcattagctg ccgcgtcagt
    15061 ggtatccact ccccgtccac cattagcaac gccagctaat gccatgattt ccgaagcatt
    15121 accacgtatc gctgccggtt taaaagataa aagttcatga caaaaatggc ggcgataatc
    15181 gagcgcacca accgctactg gatcaagcgt ccagggtgtt tgagagcttt ttgcttgctc
    15241 aacggcagca cgcatcgcct gtgcgcgtgg ctgcgtcagt gtgccaacgt taatcaacaa
    15301 ggcactggcg atagccgcaa actgactggc ttcttcggtt tcgataacca tcgctggcga
    15361 tgcaccgagc gccagtaagg tattggcagt aaaggtttgc accacatcgt tggtcatgca
    15421 gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgtg tgcgcagatt gcgcggaact
    15481 cagcaggtcg acttgcatag tttgctcctg ccataacgtg aagaagcaat gacctggtgg
    15541 tccgtgactt ccctacgctg gcattatcca gatcaggtga tacgggtatt tctcagcctt
    15601 cacgcagaag ggcaccccga gtcgtttggt tgcgatgata aatcgcagag gaggatggta
    15661 atgtccagcg cacccgttgt aaacgaggaa aaacggcagt tttacaatcg cgttaataaa
    15721 ttatttgata tatgaatcca gcaccttcag aacgacatcc agatcttctt cacgttttag
    15781 ctcatccccc tggtgaacga tgtgttccgt tagatgacct ttaatcactt cccgcatcag
    15841 accgtttacc gcgccacgga tagcagcaat ctgttgtaaa actgcagcgc attcgtgcgg
    15901 ttcgtcgagc attttcttga gcgccacgac ctggccctga atcttactgg cacgcgcttt
    15961 cagtttctgt ttatcacgga ttgtatgaga catggcaaca cctggttaac aagaacatga
    16021 aaaatcatag cactattaat ctactggggg gtagtatcag gtactggggg ggagtagaat
    16081 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag
    16141 caaggaaacg cctggttctt catccccagc gctatcttac ttggtgcgct tcatggcctg
    16201 gaaccagggc actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt
    16261 aaacaggcgg tgatgctcgg actggcagca accatttcgc ataccgcagt ggtctggttg
    16321 attgcctttg gcgggatggt gatcagcaag cgctttactg ctcaatcagc agaaccgtgg
    16381 ctccagttga tttccgcagt gatcattatt agcaccgctt tctggatgtt ctggcgtacc
    16441 tggcgcggcg aacgcaactg gctggagaat atgtacgggc atgatcacca tcatcacgat
    16501 cacgaacatc atcaagacca cgaccacgac cacgaccacg accacgacca cgaacatcat
    16561 caccatcatg aacatggcga caacgaagag tatcaggatg cccatgcacg agcccatgcc
    16621 aatgacatta aacgacgctt tgatggtaga gaggtcacca actggcaaat tttgttattt
    16681 ggcttaacag gtggccttat tccctgcccg gcagcaatta ccgtgctgtt gatttgcatt
    16741 cagttgaaag ccctgacact gggcgcaaca ctggtcgtca gtttcagcat cggcctggca
    16801 ttaacgctgg ttacagtagg cgttggagcg gcaatcagtg ttcaacaagt tgcaaaacgc
    16861 tggagcggat ttaacactct cgctaaacgc gccccctatt tttccagtct gttaattggt
    16921 ttagtcgggg tgtatatggg agtgcatggc tttatgggta ttatgagata aaacaatcac
    16981 ctgggcttcg ctatcctttt agcccaggat gattcttaaa tcagcttatt ccgctcactt
    17041 ctcgctcaat gaggatgctt ttgctacatc ctttcattta cactttttac gaaatcacgg
    17101 gatcactaac aaaatatctc ttgtcggtta tattgtatgg cgggaaagat atacgactga
    17161 ttttactgat ccccaaagtg gagagtatat gaccataaaa aataagatgt tgctgggtgc
    17221 gcttttgctg gtaaccagcg ctgcatgggc cgcaccagcc accgcgggtt cgaccaatgc
    17281 ctcgggaatt tcaaagtatg agttaagcag tttcattgct gactttaagc atttcaaacc
    17341 aggggacacc gtaccagaaa tgtaccgtac cgatgagtac aacattaagc agtggcagtt
    17401 gcgtaacctg cccgcgcctg atgccgggac gcactggacc tatatgggtg gcgcgtacgt
    17461 gttgatcagc gacaccgacg gtaaaatcat taaagcctac gacggtgaga ttttttatca
    17521 tcgctaaaaa aagccccctc atcatgaggg ggaaatgcag acaccttgtt attttttatt
    17581 attagccact tgctcgtctt gcttgttatt agtcgtattt cacgttgatt aatgcggttg
    17641 cctccagtgc gccagattta actttgtttg tatcgtagac gtagtaactg gctgttatcg
    17701 gaattgaaac cgttgtatct gtaagttttg actttgccgc aatttctttt aaatttgtgt
    17761 atcctttact gctgttagtg atatttaaaa gtgtgactgg cgaaccatta ctttcaagaa
    17821 taaatcctac gccattatct tccgcacctt tcagtattgt gttgctgttg gttaacaagt
    17881 cagaacttaa taaatacacc tgcattttcc gtgatgtttg attttgttga cctaataaat
    17941 ttttacaacg cagaataata tccccttgtt tactatttat tgttgctttg ttacctgtgg
    18001 ctggtaattg tgaaacggga atactgtcta aagtaattaa taaatcgtcc ggtttgcagg
    18061 ttgtggttat cgggttgtat gacaatgtca gattaaagga atagacacct gcattttgtg
    18121 ccaatgcatt tctgtagcta tttacacatg catcccatga cttgccacct aataaacaca
    18181 gtagaaattg tactgcggca ctcccgcgtg tcgatgatga agcactggat aatgttgcaa
    18241 ctccattaat catcactgaa ccgccgcccc ccgcagaaac attcactttt gcattactgg
    18301 ccgcaggtgt caccgttacc gaataaccac tggtgaaact ggtctgatta tttgacaacg
    18361 tgatgttatt tttattggtc cattcaaaat gtaaatttaa tttaatagta tcgttataaa
    18421 gtccaacaat aatatccgac gggctcatag gggtgatagt atttgtactt acgcatgacg
    18481 aatcattaaa attatgctca taggtcgcca tttgtgtttg agtaagtgca aaatccccct
    18541 gaacaaccaa attgtgctga acagataact cactataaca tgctgcatag ctcactggca
    18601 taaccatcat cattaatata aatagcatta tgcgaatttt catagaacca cctgactatt
    18661 actggcaaat ataatttcta ctttcatcaa tctctttacc gaaggtgatt gtgcatgaaa
    18721 gtccttgttg cttatcaatt gctacattaa ccgatggcgg tatttcattg gtgcgaataa
    18781 ataactggct tccctggccg acaacgccaa tgttatgacc atgaatatca ttgacttcat
    18841 aaccaaacgt taatggttgc ccatccgctc ttaacgcttt tataaaccag ggcttgcgct
    18901 gatcggtatc aaaattaacc agtacaaccg cgccgcgata aggggcggca attttccggt
    18961 tgccacgtaa ttctgcttcg ctatcgcttt gcgacacatc cagcatcagg tgattttccc
    19021 gataaggtgt cattccgtcg tataccacca ctccattacg gtttgttgtg cgatattttt
    19081 gcccattgac ataagcatct ttaattcctg gcgcattcat cacagcaaac gtttcggaaa
    19141 gacggttcgc cagattaacg ccacccgacc aggcgacaat gccccctgaa acactggctc
    19201 cagcctgtcg ataagtactc gactgactat aactgccatt cactgttgcc accggcgcgt
    19261 tccaggttaa attcgctcca gctgtcgttt catttccctg atgttgatga ctcaggttga
    19321 caccataatt gaactgatcc cgactcccta ctgttcctga taatcccgta ttatttgagg
    19381 caaacccctg atcatcaaac gtcgttgagt tagacatata tatttgccga cgaggcgtcg
    19441 aaacgtcatc accccaatca aagggaatcg atataaaaat attaaaacgt ttctcttcat
    19501 gatgattctc gtcataagcc tggcttgccg cgagggtata gcttatccgt cgcaggttgt
    19561 tggaataact caactgataa tccttactac tgccgctacg cccccagtaa tctcgccata
    19621 acgtacttaa tgacacagaa ccccaacctt ctggcaatga ctggctcata ttggcggaaa
    19681 ggctattttt gcgaccaaaa tcattctggt aataatcggc aatgtcatag acatcgtttt
    19741 catcacggcg ataattatct ttattgttag cccaaacgtg atcattaaat gtccgataat
    19801 cacgcgacga ataacgccag gccgccagac caaaacgcgt cgacgtttgg ctcacaaatt
    19861 tgttgtaggc aatttgataa ctttgcccgt caaatacatc gccgttgtct tgtttactat
    19921 gcgacttcgt ggcatcgacg gaaatggcac caatgcgtgt attccagcca gtccccaaag
    19981 tgaacgcgta ataattattc gcgaccatcg agccaccata cagcgtcaat aagttattaa
    20041 aaccatactg ataacccgcc tggacaaaat cactttgttt gctcgcccct tcaatatggc
    20101 tacgacccgc cgcaaaatca tatttcgaca cgccgggttg cagcatattt ggcaccgctg
    20161 cataaggcac cagataggtg gttaccgaac cgtccgcctc tttcacgctg acatcaagat
    20221 ctgctccacc accggccaac tgcaaatctg taatcgcaaa cgggccaggg ggaacctctt
    20281 tctgataaac cacaaaaccg ttctgttcaa tagttaccag cgcgttactc tgagcaatcc
    20341 cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa catctgcata tcacgaaaca
    20401 accgcacacc tctgaagcga acagaatcaa aaatatcgct tgatgtgtac atatcaccca
    20461 cgcgaagcgt gccgagaagt tgggcaaatc cacgttccag atacagggta ttgcttttcc
    20521 acgcccctgg attgttattt gttttactga aactggcatc agaatgcagt tgccacccca
    20581 gtaaatttaa cccgctgtta aaacgtacat atgtactctt actattaccc gacgctttat
    20641 agtcactgta atactgactc acataataag aggtataaaa cgcattaata ccccgctccc
    20701 agttttccgg tggaacatag ccactttcca gttcttccac ccaggcctgc gggacactga
    20761 aatcgagacg aaaaaccccg atatcccagg tatagctccc accctgaaca agttgctcaa
    20821 atgttaaaca ttgcttaccg ctggcgaagt tatcgctatt aatgcctaac cgcttgataa
    20881 cttctcttga taaacatgtt tcttgcgggt tgtctttaac aataatctca tattttccgc
    20941 gccattgctt attgacataa atatcgatgt catactgccc cggtaagggt tgattatcat
    21001 caagacgaat atttgcaacc tgctggtctt tcattccacc tatcataaaa tgggtatcaa
    21061 aggtttcttc agctgcataa gcttcaatgc cgagcaataa cgctacgatt gctgatgcaa
    21121 gtggggtcat tctcaacata ggcagctcct gcaattaaat tttgtcacta atatagttgc
    21181 cgtggtcatc gataatggtc agataccagt tatttgcatt attacttttg acattaacac
    21241 tctgactttc taagggggca atcataatag tttcataatt gactttaaca ttattagctt
    21301 tgacatccga aatcgtcacc caattagctg agtcattttt tatcaccaat ccattgccac
    21361 tgcgatttac cagtaacttt ttaaaggtcg ctttatttac cggagcaata cccgccggcc
    21421 ggtaaaacaa tttgattctg ttttgcatcg caaacttcag tgcattctta ccttcttgtt
    21481 ctggactatt tggtggaata tccagaacat tcagataaaa aatgctttct ttattagtgg
    21541 gcagtttatt cggcataatt ttgattttta cttgctgccc ggaatttgcc cctatttttg
    21601 ccactggtgg cgttaacatg aaaggaacct gaattttttc tggtggtaac gacgtatcgc
    21661 catcatcaat ccacgcctgc agcagcgaag aacggtttcc ctggttcatc aactgcacca
    21721 taacttcttt attttctgcc gggtaaataa tgcgcgtccc gtagataaca attccggcat
    21781 gtgcaggaag gaccatagaa aaaatgagta aagataataa tcctttcatc ttaatgcctc
    21841 ttaaattaaa aagccgccat ggggatagcg gccatgttta ttacctggag aaacttgcaa
    21901 ttttatttat taatcctgcg ccacacgcat aaccagagaa gataatacat cacctgattg
    21961 cactgagtta tctgtcagtt tcaggtattt tgctttaaac ggaatagcat attgaccttc
    22021 cgctgtttta taactctcaa tattaaaagg tgcagaaaca tccaggacat cgctgttatc
    22081 acctaaatag agatagtaac ccacagtatc gttgctactg gctatcgcct tatcgttacc
    22141 agttatttta tcgttggtaa tcaacgagag ttttacacca gcaacttctt tcgggcagct
    22201 aatttttaac atgctgctgg ttacaccgac tgcatcatca taggtagaat atttagcttt
    22261 accttcaaag acagtggttg gtacagtcgg catattaata cttactgaat caccacccgc
    22321 ttcgaattta cacggagatt ctgcaatatt ccctttaata gttaatgttc cggtatcaac
    22381 gtctgcagca aatactccag cgctcataaa aaaagaagaa aagacagcga cagcaa
//
LOCUS       DAPGRN010000078        22379 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPGRN010000078 DAPGRN010000000
VERSION     DAPGRN010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.078"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            120..551
                     /gene="osmC"
                     /locus_tag="RHW33_003125"
     CDS             120..551
                     /gene="osmC"
                     /locus_tag="RHW33_003125"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV1646605.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(586..1356)
                     /locus_tag="RHW33_003126"
     CDS             complement(586..1356)
                     /locus_tag="RHW33_003126"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDV1646606.1"
                     /translation="MNLQGKTALVTGSTSGIGLGIAQTLANAGAQVILNGFGDVDAAI
                     NAVSRMGKTPGYHEADLSDESQIINMVAYARREFGGVDILVNNAGIQYVSPVESFPVE
                     KWNAIIAINLSSVFHTTRLALPEMRTRNWGRIINIASVHGLVASKDKSAYVAAKHGVV
                     GLTKTIALETAQTGITCNALCPGWVLTPLVQQQIDKRIAQGEDAESARDALLAEKQPS
                     REFVTPQQLGELALFLCSDAAAQVRGVAWNMDGGWVAQ"
     gene            complement(1368..2759)
                     /locus_tag="RHW33_003127"
     CDS             complement(1368..2759)
                     /locus_tag="RHW33_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDV1646607.1"
                     /translation="MSVLIALAALGLLMLAAYRGYSVILFAPIAALGAVLVTDPGAVG
                     PAFTGLFMEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAINILGRRHAIPVI
                     VLVCALLTYGGVSLFVVAFAVYPFAAELFRQSNIPKRLIPATVALGAFSFTMDALPGT
                     PQIQNIIPTSFFGTNAWAAPWLGLIGSLFIIAVGLIYLERQRRKAQAKNEGYGTELLN
                     EPETPDNINLPNPLIAISPLILVGLLNLLFTWWIPVWYGASHQLELPGLAAPITTDVA
                     KITAIWAVEAALIAGILLVVIFGYRNIHGRLAEGSRTAVGGAILAAMNTASEYGFGAV
                     IAALPGFLVLSKALAAIPDPLLNEAISITTLAGITGSASGGMSIALAAMSDTFIAAAH
                     AANIPLEVFHRVASMASGGMDTLPHNGAVITLLAITGLSHRQAYGGIFAITIIKSLAV
                     LFIIGVYYTTGIV"
     gene            complement(2805..3728)
                     /locus_tag="RHW33_003128"
     CDS             complement(2805..3728)
                     /locus_tag="RHW33_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HDV1646608.1"
                     /translation="MQTEQFAVLGAGLMGVGIATHFARFGHDIWLYDTEQSRIAEIHV
                     VAGGILDELISCEQFTASEKENVLKRLHGTTSLADIADSQLLIEAIPERLELKHALYT
                     ELETLISPEAIVASNTSGLPPDDLAAKMRYPQRLLIAHFWHPPHFIPLVEIVPGSVTE
                     AKHLHWLQHLLVSLQLEAVLLDRAAPGFVGNRLQFALLREALHIVKSGIASAEVVDQV
                     MRASLGRRYAMVGPLEAADMTGLSTVQDICRHLLPELATGSDMMSLVAEKVERGDIGV
                     RSGQGFYRWDESRKQYIQQRREHQLRFALKP"
     gene            complement(3718..4923)
                     /locus_tag="RHW33_003129"
     CDS             complement(3718..4923)
                     /locus_tag="RHW33_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV1646609.1"
                     /translation="MQDVVIVAATRTPIGCFHGALAPLSAVDLGAATIRSLLEKTALA
                     AEQIDEVIFGQVLTAGSGQNPARQSALKAGLSHDTPAVTINLVCGSGLKAVHQAVQAI
                     RCGDAQIIIAGGQESMSNAPYFIDGARAGLRLGHTSMKDSVVHDGLWDAFNDYHMGIT
                     AENLAQKYAISRERQDTFALRSQQKAAAAMEAGRFADEITPVSVPQGKKPPRWVNTDE
                     QPRSGTQLEKLAQLKPAFLPGAGSVTAGNASSINDGAAAVMLMRASKAAELGLPVLAR
                     VAGYAVSGVDPAFMGIGPVAATRECIKRCGWTLEDIDLIEANEAFAAQALAVGDELNW
                     DSDKVNVNGGAIALGHPIGASGCRILVTLIYEMARRNAQKGLATLCVGGGQGVALTLE
                     RTTGEHHAN"
     gene            complement(4933..5589)
                     /locus_tag="RHW33_003130"
     CDS             complement(4933..5589)
                     /locus_tag="RHW33_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HDV1646610.1"
                     /translation="MLTREQMAMRVARELRDGYYVNLGIGIPTLVANYIPDGMDVMLQ
                     SENGLLGMGAFPTEDELDADMINAGKQTVTARKGAAIFDSAQSFAMIRGGHVDLTVLG
                     AFEVDVNGNIASWMIPGKMVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPDCTLP
                     LTGVGCIKRVLTDLALLEIADGAFILRERAPGISVAEIVAKTAGKLFVPEHVPEMQFN
                     "
     gene            complement(5592..6299)
                     /locus_tag="RHW33_003131"
     CDS             complement(5592..6299)
                     /locus_tag="RHW33_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDV1646611.1"
                     /translation="MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRH
                     RGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASYVGENALFEKQVINGELEAILTP
                     QGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
                     KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQ
                     GQFEKRIEQRTLSRKGA"
     gene            6441..7331
                     /locus_tag="RHW33_003132"
     CDS             6441..7331
                     /locus_tag="RHW33_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1646612.1"
                     /translation="MKMSIKQLRAFLAVAHTLNFAHASERLNMSQPALSLAIKGLEDA
                     LGGALLLRTTRKVTLTQEGETLLSMARPLLADWDNTEEAMRQRFTLRRGKVSVAAMPS
                     FAANVLPPVLKTFRDCYSGINVTVHDVVNEQVIEMVSEGRVEMGIAFEPDYCGHVHFT
                     PLGVDRFVAIVPADSRLANRQRVSWRDLLTLDFITLQRPSAVRLMLEEQLAQSGTQLE
                     VALESHQLVTVGRLVANGLGGSAVPALCRRQMSELGAVCLELDNPVIERRVGVLRSSH
                     HKLSTAAQALMETLKKTYDA"
     gene            complement(7368..8294)
                     /locus_tag="RHW33_003133"
     CDS             complement(7368..8294)
                     /locus_tag="RHW33_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1646613.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTELPGNRTLPQGCFF
                     RERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(8287..9273)
                     /locus_tag="RHW33_003134"
     CDS             complement(8287..9273)
                     /locus_tag="RHW33_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1646614.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGIPRQPLHAIPGT
                     VPNLTHLPDGCAFRDRCFATGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(9270..10166)
                     /locus_tag="RHW33_003135"
     CDS             complement(9270..10166)
                     /locus_tag="RHW33_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV1646615.1"
                     /translation="MMLSEETSAVRPAKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(10163..11185)
                     /locus_tag="RHW33_003136"
     CDS             complement(10163..11185)
                     /locus_tag="RHW33_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1646616.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAAWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGDE"
     gene            complement(11187..12735)
                     /locus_tag="RHW33_003137"
                     /pseudo
     CDS             complement(11187..12735)
                     /locus_tag="RHW33_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(12749..13330)
                     /gene="ddpX"
                     /locus_tag="RHW33_003138"
     CDS             complement(12749..13330)
                     /gene="ddpX"
                     /locus_tag="RHW33_003138"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV1646617.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(13588..15987)
                     /gene="dosP"
                     /locus_tag="RHW33_003139"
                     /gene_synonym="pdeO"
     CDS             complement(13588..15987)
                     /gene="dosP"
                     /locus_tag="RHW33_003139"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV1646618.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(16012..17394)
                     /gene="dosC"
                     /locus_tag="RHW33_003140"
     CDS             complement(16012..17394)
                     /gene="dosC"
                     /locus_tag="RHW33_003140"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV1646619.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSNYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(17432..17473)
                     /locus_tag="RHW33_003141"
     CDS             complement(17432..17473)
                     /locus_tag="RHW33_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646620.1"
                     /translation="MVQLVDLARCVSF"
     gene            complement(17771..19090)
                     /locus_tag="RHW33_003142"
     CDS             complement(17771..19090)
                     /locus_tag="RHW33_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV1646621.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(19221..20756)
                     /gene="gadC"
                     /locus_tag="RHW33_003143"
     CDS             complement(19221..20756)
                     /gene="gadC"
                     /locus_tag="RHW33_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV1646622.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYLELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSKNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(20912..22312)
                     /locus_tag="RHW33_003144"
     CDS             complement(20912..22312)
                     /locus_tag="RHW33_003144"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV1646623.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
BASE COUNT         5512 a         5827 c         5694 g         5346 t
ORIGIN      
        1 atttaattta agtgcagaag taatattttt gccgtatttt attcggaata tcctgcttat
       61 cctcgtgcgg tttctcacgt agtctataat ttccttttta agcccacagg agagcaacaa
      121 tgacaatcca taagaaaggt caggcacact gggaaggcga tatcaaacgc gggaagggaa
      181 cagtatccac cgagagtggc gtgctgaacc aacagccgta tggatttaac acgcgttttg
      241 aaggcgaaaa aggaaccaac cctgaagaac tgattggcgc agcgcatgcc gcatgtttct
      301 caatggcgct ttcattaatg ctgggggaag cgggattcac gccaacatcg attgatacca
      361 ccgccgatgt gtcgctggat aaagtggatg ccggttttgc gattacgaaa atcgcactga
      421 agagtgaagt tgcggtgccg ggtattgatg cctctacctt tgacggcatc atccagaaag
      481 caaaagcagg atgcccggtc tctcaggtac tgaaagcgga aattacgctg gattaccagt
      541 tgaaatcgta aagcattgcc ggatgacgcg tcatccggcg aacacttact gcgctaccca
      601 accgccatcc atattccacg ccaccccacg cacttgtgct gccgcatccg aacagagaaa
      661 cagcgccagt tcacccagtt gttgcggagt cacaaattca cgagaaggct gtttttctgc
      721 cagtaaggcg tcacgcgcgc tttccgcatc ttcgccttgc gcaatgcgct tatcaatctg
      781 ctgttgtacc agcggcgtca gcacccagcc agggcagagc gcgttgcagg taatacccgt
      841 ctgcgcagtt tccagagcaa tggtctttgt gagtcccacc acgccatgtt ttgccgcaac
      901 ataagctgat ttgtctttcg acgctaccag cccatgcaca gaggcaatgt taatgatccg
      961 accccagtta cgagtgcgca tttctggcag tgccagacgc gtggtgtgaa agacagaaga
     1021 gagattgata gcgataatgg cgttccattt ttctacaggg aaagattcaa ccggggagac
     1081 atactggatc ccggcgttat tcaccagaat gtccacaccg ccaaattccc gccgtgcgta
     1141 ggccaccatg ttgataattt gcgattcatc gctgaggtct gcctcatgat agccaggcgt
     1201 tttacccata cgagaaaccg cattgattgc cgcgtcaaca tcaccgaagc cgttgaggat
     1261 cacttgcgcg ccagcgtttg ccagtgtctg agcaataccg agtccgatac cgctggtgga
     1321 ccccgtcacc agcgcagttt taccttgtaa attaatgatg attttcctta gacaatgccg
     1381 gtggtgtagt acacgccgat gataaacagc acggccagac ttttaatgat cgtaattgcg
     1441 aagatgccgc cgtaagcctg acggtgactc agcccagtaa ttgccagcag ggtgatcaca
     1501 gcgccgttat ggggcaatgt atccatgccg ccactggcca tagatgccac gcggtgaaaa
     1561 acttccagtg gaatattggc agcgtgcgca gcggcaataa aagtgtcgga catagccgca
     1621 agagcaatac tcatcccgcc ggacgctgaa ccggtaattc cggcaagtgt ggtgatgctg
     1681 atggcttcgt tgagtaatgg atcaggaatg gcggccagcg cctttgagag caccagaaaa
     1741 ccgggtaggg cggcgataac cgcaccgaag ccatattctg aggcggtgtt catcgcagcc
     1801 agaattgcgc cgcccaccgc tgtacggctt ccttctgcca gtcttccatg aatattgcga
     1861 tatccaaaaa tcactaccag caaaattccg gcaatgagcg cagcttcgac ggcccagatt
     1921 gcggtgattt tggcgacatc tgtggtaatc ggggcggcaa gtcccggcaa ctccagttgg
     1981 tggctcgcac cgtaccaaac agggatccac caggtaaaca acaagttcaa cagccccacc
     2041 agaatcaatg gtgaaatcgc aatgagtggg ttcggtaaat tgatgttatc tggtgtttca
     2101 ggttcattaa gtaactctgt gccgtaacct tcattttttg cctgcgcttt acgacgctgc
     2161 cgctccagat agataagccc gacggcaatg ataaacagtg aaccaataag ccccagccac
     2221 ggcgcggccc aggcgttggt gccaaagaaa ctggtaggaa taatgttctg aatttgtggc
     2281 gtgccgggca gagcgtccat agtaaaagag aatgccccca gggccaccgt tgccggtata
     2341 agtcgttttg ggatgttact ctggcggaac agttcagccg cgaacggata aacggcaaat
     2401 gccaccacaa acagcgacac tccgccatag gtgagtaatg cacataccag cacaatcacc
     2461 ggaatcgcat gccgccggcc gagaatgttg attgcagcag cgacaatcga acgagaaaat
     2521 cctgaaagtt caatcagttt tccgaacacg gccccgagca gaaacaccgg gaagtacaat
     2581 ttcacgaagc cgaccatttt ttccatgaac aggccggtaa atgctgggcc aacggccccc
     2641 ggatcggtga caagaacagc gcctaatgct gcgatggggg caaaaaggat aacgctgtat
     2701 ccgcgatagg ctgccagcat tagcagccca agcgcggcaa gggcgattaa tacactcatc
     2761 gggactcctc aatatcgttg ttatagtggg tacagagaga gaagttatgg tttcagggcg
     2821 aagcgaagtt gatgttcccg acgctgctgg atgtactgct tccgcgattc atcccagcgg
     2881 taaaaaccct gaccgctacg tacgccgatg tctccacgtt ccactttttc ggcaactaac
     2941 gacatcatgt cgctgccagt cgccagttca gggagcagat ggcgacaaat atcctgcacc
     3001 gtcgaaagtc cggtcatgtc ggcggcctcc agcgggccga ccatggcata acgtcgccca
     3061 agcgaggcgc gcatcacctg atccaccact tcggcactgg cgatgccgct ttttacgata
     3121 tgcagggctt cacgcaacag tgcaaattgc agacggttgc cgacaaatcc tggcgcagcg
     3181 cgatccagca acacagcttc cagttgcagt gacacaagca agtgttgcag ccagtgcaaa
     3241 tgctttgcct cggtaacgct gccggggaca atctccacaa gtggaatgaa gtgcggcgga
     3301 tgccagaaat gggcgattag cagtcgttgc gggtagcgca tcttcgcggc cagatcatcg
     3361 ggcggcagac cgctggtatt gctggcgaca atggcctcag gagaaataag cgtttcaagc
     3421 tcggtatata atgcgtgttt tagttcaagt cgctccggga tcgcctcaat cagtagttgc
     3481 gagtcggcga tgtctgcaag agaagtcgtg ccgtgcagac gtttaagcac attctctttt
     3541 tccgaggcag taaactgttc gcagctaatc agctcatcaa gaattccgcc tgcaactacg
     3601 tggatttctg caatccggct ctgctcggta tcgtacagcc agatatcatg accaaagcgg
     3661 gcgaaatgag tggcaatccc cacgcccatc aatccggcgc ccagtaccgc aaattgttca
     3721 gtttgcatgg tgttctcctg tggttctttc cagcgtcagc gccacgccct gaccgccacc
     3781 gacgcacagc gtcgccagcc ctttttgcgc attacgtcgc gccatttcat aaatgagagt
     3841 gaccagaatg cggcaaccgg aagcgccaat aggatgccca agtgcgattg cgccgccgtt
     3901 aacattcact ttgtcgctat cccagttcag ttcgtcgcca acggccagcg cctgtgctgc
     3961 gaaagcctcg ttggcttcaa taagatcaat atcttccagt gtccatccgc aacgcttaat
     4021 acattcgcgg gtagcggcga ccggaccaat gcccataaag gcaggatcga cgccagaaac
     4081 ggcataacca gccacccgag ccagcactgg gaggcccagt tcggcagctt tgctggcgcg
     4141 cattaacatg actgcggcgg caccatcatt gatggaagaa gcatttcctg cggtgacgct
     4201 tcccgccccc ggaagaaagg ccggtttcag ttgtgctagt ttttccagtt gcgtcccgga
     4261 acgtggttgt tcgtcggtat ttacccagcg tggtggtttt ttaccttgtg gtacgctcac
     4321 cggagtaatt tcgtctgcaa agcgcccggc ttccattgcg gcagcggctt tttgctgaga
     4381 acgtagcgcg aatgtgtcct gtcgctcgcg actgatggca tatttttgcg ccaggttttc
     4441 tgcagtaatg cccatgtggt aatcattgaa agcatcccat agtccgtcat gcaccacgct
     4501 gtctttcatg ctggtatgcc cgaggcgtaa acctgcccgc gcgccatcaa taaaataggg
     4561 cgcgttgctc atgctttcct ggccacctgc gataattatc tgtgcatcac cgcaacggat
     4621 cgcctgcact gcctggtgta cggctttcag accggaacca cataccaggt taatggtaac
     4681 ggcaggtgta tcatggctta acccggcttt gagcgccgat tgtcgtgccg ggttttgtcc
     4741 gcttccggcg gtcaggacct gaccaaaaat cacttcgtcg atctgttctg ctgcaagtgc
     4801 ggttttctcc agcaacgaac ggatggtggc agcaccgaga tcaaccgcag aaagtggggc
     4861 gagagcgcca tgaaaacagc caatcggcgt gcgcgtagcg gcaacaataa caacgtcctg
     4921 catggtgatt cctcagttga attgcatttc cgggacatgt tccggcacaa acaacttacc
     4981 ggcggttttg gcgacgattt ccgcaacgct gattcccggc gcgcgctcgc gcagaatgaa
     5041 agcgccgtca gcaatttcca gcagcgcaag gtcggttaag acgcgtttga tgcagccaac
     5101 gccggtcaac ggaagggtac agtcaggaag cagtttggat tcgccggatt tcgaggcgtg
     5161 ggtcattacc acaataatat tctcggcacc ggcaaccaga tccatggcac cgcccatgcc
     5221 tttaaccatc ttgccgggga tcatccatga ggcaatgttg ccattcacat ccacttcaaa
     5281 tgcgccaagc acagtcagat ccacatggcc gccacggatc atggcgaaag attgcgccga
     5341 gtcgaaaatt gccgcacctt tccgtgcggt aacggtttgt ttaccggcgt tgatcatgtc
     5401 ggcgtccagt tcgtcttccg taggaaatgc gcccattccc agcaaaccat tttctgactg
     5461 caacatcaca tccatcccgt caggaatgta gttggccacc agtgtcggga taccaatgcc
     5521 gagattgacg taatagccat cgcgcaattc gcgggcgact cgcatcgcca tttgttcacg
     5581 ggttaacatg gtcaggctcc tttgcggcta agggtgcgtt gttcaatgcg tttttcaaat
     5641 tgcccctgaa tgacgcgatt aacaaaaatc cccggggtat ggatagcggc aggatcaagt
     5701 tcaccgggtg gcactatttc ttccacttcc accacggtga tttttccggc agcagccatc
     5761 agtgggttaa agttttgcgc ggtatgacga taaatcacgt tgccgtacca gtcggctttc
     5821 cagcctttga ccagggcaaa atcaccggtg atagcctgtt ccatgatgta cgggcgaccg
     5881 ttgaattcgc ggacttcttt cccttcggca acgggagtac cgtaaccggt ggcagtaaag
     5941 aacgccggga tacccgcgcc gcctgcgcgc agttgttctg ccagggttcc ttgtggtgtg
     6001 agaatcgctt ccagttcgcc gttaatcacc tgtttttcaa acaaagcatt ctcgccaaca
     6061 taagaagcga cgactttacg gatctgtcgg ctttccagta gtacacccag tccaaaacca
     6121 tctacgccac agttattgga aaccaccgtt aatccggtta ctccacggtg gcgaacttca
     6181 gcaatcagat tctccgggat gccgcacagg ccaaaacctc ctgccagaat cgtcatctca
     6241 ttggtaagtc cttcaagcgc ctcgtgatac gtagcgaccc gtttatccaa cccagccata
     6301 cagttgccct ccagttgtcc ttttcaacat cattaagggc aaaaaatgat ttgttaattt
     6361 taaatttgtg actcattcat ttgtattatt tatcaatata atgttaatta aatgtattca
     6421 gcaggttaac ggagtgccag atgaaaatga gcatcaaaca gttacgcgcg tttttagcgg
     6481 tagctcacac tctgaacttt gcccatgcca gtgaacggct gaatatgtcg cagcctgcgc
     6541 taagtcttgc cattaaagga ctggaagatg ccttaggggg cgctttactg ctgcgtacta
     6601 cgcgaaaagt gacattaacc caggaggggg aaacgctatt gtcgatggca cggcctttgc
     6661 tggctgactg ggataacact gaggaggcca tgcgtcagcg attcacgctc aggcggggca
     6721 aagtctcggt ggcagcgatg ccgtcatttg cagccaacgt tctgcctccg gtgctgaaaa
     6781 ccttccgcga ttgttattca gggattaatg tcaccgtgca cgatgtggtg aatgagcagg
     6841 tgatcgaaat ggtgagcgaa gggcgtgtgg agatgggtat tgcctttgaa cctgactatt
     6901 gcggccatgt gcattttacc ccactgggcg tggatcggtt tgtcgctatt gttccagccg
     6961 attcacgact ggcaaatcgg caacgcgtga gttggcggga tttgctgacg ctcgatttta
     7021 tcaccctcca gcgaccgtca gccgtcaggt tgatgctgga ggagcagttg gcacaaagtg
     7081 gaacgcaact ggaagtggcg ctggaaagcc atcaactggt tacggttggg cggctggtgg
     7141 caaacggttt gggaggaagc gctgtgcctg cgctgtgccg caggcagatg agcgaactgg
     7201 gcgcagtgtg tctggaactg gataatccag tgattgagcg acgagtaggc gtactgcgtt
     7261 cgtcacacca taagctttct actgcggcgc aggcgttgat ggaaacgtta aaaaaaacct
     7321 atgacgcata acgtttccgc agacatgcaa tagccggatg ccgcgtccta cagcgcccgc
     7381 cagcaacgga tctcacgtcc gtcagcccgc gtcgttaatg attgccggac ttcgcatccg
     7441 ctggttgcaa gggggcaacg ttcgcggaaa aaacagcctt gcggcaacgt gcggtttccc
     7501 ggcagttccg ttttacgtaa tgcccattct tcctccagcg gtttatcaat ggcggggagg
     7561 gaatccagca ataatcgggt gtatggatgt gccggagccg ttagcacctg ttgcgcttcc
     7621 cctaattcca caatctgccc gagatacatc accgctaccc gatcgctcat atgacgaatc
     7681 accgagacat tgtgtgagat cagcacatag gtcagcccgc gatttacctg tagcgttacc
     7741 agtaaattga ggatctgcgc ctgcacggag atatccagcg ccgaggttgg ctcatcaagc
     7801 acaatcacgt caggctgcga agagagtgct ctggcaatgg cgatgcgttg ccgctgcccg
     7861 ccggagaacg catgaggcag gcggtcgaga tactccggac gaatacccac ctgcacagcc
     7921 agctcctctg ccaacgctcg ccgctgttgt tcactactac gcttagctat ccagagcggt
     7981 tctgtgatga tgcgccacac cggtaagcgc ggattaagcg aagagagcgg gtcctgaaac
     8041 accatctgca taatgcgttg tgatcctgaa cggatgtatt gcccgtggct cggttgcagc
     8101 atacccatta aaagctgtgc gagggtgctt ttgccgcagc ctgactcacc gacgatccct
     8161 aaggtttcac cacggcggat ctgtaaatca ataccattaa tcgcatgaac atgttccgta
     8221 gttttaccaa gccagttttt acgggccggg aaattgatat ggacgtcgcg taacgttaat
     8281 aacgtgtcag acacttatga cctcctgctg cggataccaa caggcgcagc gctggttgtt
     8341 gtcaccacac gctgtcagcg ccgggacgtt ttcacactgt gcacctgtcg caaagcaacg
     8401 atcgcgaaaa gcgcagccgt caggcaaatg ggtgagattt ggtaccgtcc ctggaatggc
     8461 gtgtaatggc tggcgcggta ttccatgttc tggtgcgcat tgcagcaaac caatggtata
     8521 cggatgccgg ggatgatgga taacgtctgc cgtcacgccg ctctcaatca cgcttccggc
     8581 atacatcacg taaacgctat cgcacagttg cgacaccacg gccatatcat ggctgatgaa
     8641 cagtaccgca gttccactgg cgcgagcttt atgtttaagc aaacgcagta cctgcaactg
     8701 taccgtgacg tccagcgccg tagtcggttc gtcggcaata atcaattgcg gctcgcagga
     8761 gaatgccagc gcaatcatta cccgctggcg cataccacct gaaagctcaa acggatagcg
     8821 cgacataact tccacggcat ccgggatttg catctcttcc agcagggcaa tcgctttagc
     8881 tctggcttcc cgacgactta ttggttgatg atggcggatc acgtccatca tctgaagacc
     8941 tattcgacgt gtcggattga gggcggtcat cggttcctga aagatcatcg ccactcgtgc
     9001 gccgcgccac tgacgaagct gtttttcccg cgcgttaaga acatcgtctc ccagcagtga
     9061 aatctgcccc cgatgtacgc aataactgcc cgtcggtagc agacgcataa tcagcattgc
     9121 ggtgactgat ttacctgagc cggattctcc caccagaccg acaatttcac cgcggttaat
     9181 ctgcaaggac acattgttga gcgcgtgaac atcaccgtta aaaccgggga aactcaaatg
     9241 cagttgttga atgtccagaa cgggttgggt catgactgct ttcctcctgc tttcgggtca
     9301 agcagatcgc gaataccatc accaaagaga ttaaacccga cggcggtaag caaaatcgct
     9361 gcccccggaa atgcgcaata ccaccactga tcgagtacat agttgcgacc atttgctacc
     9421 atcgcgcccc attcagcact cggttgttga gcacccaggc caataaatcc caacgtggcg
     9481 gccattaaaa tcgcgctacc gatatccagc gatgcctgca cgatcagcgg cggcagggag
     9541 ttacgtaaaa tatgccagct gatcagatgc caacgagacg caccaaaggt tttcgccgcc
     9601 tgaacatagg tatactggcg tacaactaat gcttgccccc gcgccaggcg cacataaaag
     9661 ggaattcgca caatagcaat cgccagcatg gcgttaaata aactcggccc gagagcggca
     9721 gccagtgcca ttgtcagtac caacgaagga atcgacagca taatgtccat gatgcgcatg
     9781 atgatggcgt ctgcgcgtcc gccaagcaca ccggatagac atccgagtag cgaaccaatc
     9841 atacccgcaa tggcaaccac gactaatccg gcgagaattg attgctgact gccgaccagt
     9901 acgcggctga acagatcgcg tcccacttca tcggtgccaa accagtgcgc cgcagaaggc
     9961 ggcaaaaggc gggcggttaa atcaatggcg ttgggatcat gcgtcgcaag ccacggtgaa
    10021 aaaatcatca tcaatagcat taatacaatg atgactgcgc cggtcacggt gagcggactg
    10081 cctttcagca tccataccag ttttgcaccg ttaaatcgcg tttgttttgc tgggcgtacg
    10141 gcggacgttt cctcgcttag catcattcat cacctccacg tccgatacgc ggatcaatcc
    10201 acaaatagag caaatccact accaggttca ccagcacata agcaaacgaa accacgacgg
    10261 caaatcccat caccgcaggg aagtcgagcg cctgtattga tgtcactacc catgccccca
    10321 ttccgggcca ggcaaaaacg gtttcggtga gcactgcgcc atacaacaaa tcgcccagcg
    10381 ccagacccaa tacggtaatc gatgggatca acgcattggg tagcgcataa cataaaacga
    10441 tataccagcc gggcaagccg ctggcccggg cggtacgaat gtagtcttca ctcaattgtt
    10501 ccagcatcgc tgagcggatc tggcgagcga caattcccag gtgaacgaac gccagcgtta
    10561 atgccggtaa gatgagatgt tgcaacgcat tgaagaagac ttcaccgttg ccttcaagca
    10621 gcgcatcgag cagataaaag ccggtaacgt gcgttggtgg atccagccag tcatcaagcc
    10681 ttccgccgcc gggaagaatt tgcagatgac cataaaacag cacaatgacg cccagtccaa
    10741 gccagaacgc aggtgtggag attccggtaa tggccattat tcgcaccaga tgatccagcc
    10801 agcgatttcg ccaggctgca gagagtatgc ccagcgggat gccaatcaag agtgccagca
    10861 gcagagcaca aaaagccagc tccagcgtcg ccgggaaaaa tatacgcaac tcttccagca
    10921 ccggacgccc ggtacgaatg gatgttccca ggtcaccatg aaacaggtcg ctgacgtagc
    10981 ggtaaaactg tacatacagt ggctggtcca gtcccagttg ctggcgaata ttttccacga
    11041 tagcatcgct ggcgcggtca cccgccagta accgcgccgg atcgccgggg atcagatgcg
    11101 agataataaa cgtaatcacg cagacgcccg ccaccacgag caccagcccc cagcagcgtt
    11161 ggcgtaaaat actccagaag gtcacgttat ttactcatgg tattgatatt gaagacctgt
    11221 tccagcatgg gattgaacac aaagcctttc acctctttgt tcatcgccag ttggtagttt
    11281 ttctggaaca ggtacacata agcagcgtca tcaatgacga ttttctgtgc ctgctggtag
    11341 ttccgggtac gctgcgtctg gtcggtggtc gcgagcgcat tgcgcagtaa cttatcgacc
    11401 tcactgtttt catagaacga gcggttaccc ggcagacctt ttttgtctga ctcaaaccag
    11461 taattcataa acatatacgg gtcggcaaaa tccggactcc agttgccaat cgcaatgtcg
    11521 taatcacctt tacccactct cgcgcatggt ggcgttcgcc agcttttcca gcttcacatt
    11581 gatgcccagc ttgttgagac tggattgtgt cgccagagca ataggctccc agttcggatc
    11641 gttatcggag tagagaaacg tcaggctggt gggtttgctc gtcactttat cccattcggc
    11701 tttggctttc gtttcgtcat ggttgtattg cattgccgtc gcatcgtagc cccacatgcc
    11761 ttccggaatc gggccgcgca tttgttttcc gttaccactc agaatgccgt taaccatgcc
    11821 ctgataatcg gtagaccagg aaatggcccg ccgcagctcc gcctgattaa gtggcgcttt
    11881 gctgttattc agatacagat aggtgacgcg cagtgacgga tactctgcca cattgacttt
    11941 gttttcctgc ttcagggcgt tgagttgatc caccggcagc gcatcggcaa tgtcaatgtc
    12001 gccacgggag agctgcaggc gacgggaggc actttcaccg ataattttta ccgatactcg
    12061 cttaaagttc ggtttattgc cggagtaatg cggatttggc accagaacta attgctgacc
    12121 tttttgccag cttttcagca taaacggtcc ggagccagcg gtattttgcg cgaggaagcc
    12181 gcgggcatca tccgccgcat gttcctttaa caccgccgga ttgataatgg atgcaccgtc
    12241 attcgccagc gtgtagagga acggtgcgaa tggttggcta agggtaaact tcaccgtatg
    12301 ttcgtcggga gcatcaatct ttaaatcttt gggaaatgct tctgctggcc cctggccgat
    12361 ttttagtagc cgctcaaaag aaagttttac tgcttcggca gtgacaggtg tgccatcagc
    12421 aaatttagca ttatctttca gggtgaacgt ccactctttt tgatcgtcag acgctttcca
    12481 gctacttgcc agatcgcctt caacgtcggt tgagccttta tcaccgtccg ttttgtactg
    12541 aaccagccgc tgataagacg ggtaggtcac tgtccagtcg ttattatcta ttgttaccgc
    12601 cgggtcgagg gtttgtggat cggcggcctt accaatcacc agcatatctt tcggtacggc
    12661 ggcgtgcgca accgggaaag ttgtggcaag gacgagcgcg agcaatgtgg gacgaaacga
    12721 tatcgatctc ttcatgacag tttcctgatt aactgacgtg ctgcgtgcca ggagatataa
    12781 aacaggtgaa ttgatcagcg agcagagggt aactcgctgc ctggggtaat tcgaagtgcc
    12841 accattcgct ggagataccg acaaaaccgc caccagtcat tatcgcattc agcaacaggc
    12901 gattgcgctg agcggcgggc gggacggaag ggtgataggc atgggagcgc tcgtgcattt
    12961 catcgaaccc tgcgcccata tccaggatgt tcccgtgctc atcacgaagc gtcaggtcga
    13021 tcgccgtgcc acggctgtga ttagaaccga ccgtcacatc aacaacatat tgcgggtctg
    13081 ggcaggcttg ccacaacatc gcctgcgctt gttgtgggcg atacgcatcg taaatcacca
    13141 gttgtaagcc tgatagctgg gcgatactga tacttttcgc cagcgcggta atcgcatcct
    13201 tgtgtaacag acaacgcgct tgctggtaaa tagctttacc tgtgatgtta tcagcgcagg
    13261 cgtatttcaa ttcgatctcc agatcaggga agatcacggc taaatcaacc agttcggtgg
    13321 tatccgacat aaacggttcc tgtataagac aaaaattgct gcgctttccg cttatgcaga
    13381 tctcatgcca tgccgggata agcgccagaa tgctggctta aagttatatt ttttaacttt
    13441 tgatcaacat ttgtgcagcg tagtgcagtt ttggtgcaag aggggaagtt aaggaaggaa
    13501 tctcccggaa tcgtagctga aatcacagta tttaagtgac agtgtcacgt taaatgaaaa
    13561 cccgcgagtg cgggcgagag gaatttgtca gattttcagc ggtaacacgc tgctcatcca
    13621 gcctggaatt tcttcggcgg gtagggggcg ggaaaagaaa tatccctgaa taacgcgaca
    13681 gtggatcttg cgtagcatct caaattgctc tttggtttcg acgccttccg ccacgacggt
    13741 taaattgagg ctttgcccaa tgctggtaat ggcttcaagt aaggcaagga tgcgtttttc
    13801 cgtcagacaa cgatcgacaa aacttttgtc aattttgatt tccgttaccg gaagactgac
    13861 taagcgggat aatccggaaa agcccgtgcc aaaatcatct accgataaac ccacgcccat
    13921 atcacgcagg atctgaatgc gcttaaagat ttcggtatcg tgttccatca tcatgctttc
    13981 cgtgatttcc accgtcagct ggtggccgtc aataccccag gcttgcattg catcagacac
    14041 ctgattaggc agttgattac tgcgaaagtg cagcgccgac aagttcacgg ataacgccgg
    14101 gatatgaata ttctggctac gccattctgc taactgacgg caagcttccg cgatgaccca
    14161 gcgcccaata ttttcgattt caccaatctc ttctgcgaga ggaataaacc gtgaaggggg
    14221 cacatgacca tgctggggat cgtgccagcg agcaagggct tcgatgccgt agagttcacc
    14281 cgtttctgcg aagatttgcg gctggtaaac cagtttcagt tggttattgc taatcgcttc
    14341 tttcagcgct gcgcctaaaa ccaaacgctc ttttaccatt tcgttcatcg ccgggctgaa
    14401 gaactgccag ccgttaccgc cattcttgcg aatataatcc attgcattgt gagcagtgga
    14461 gagcaggtaa tcgcggtttt tacccacgtc gtagctgatg ccaatactca aggtaagcgg
    14521 gaagggttta tcgtcaatca ttatcggctt gctgaccaca ttccgtagct catcggcgat
    14581 ttgggtaatg ttactgacgt cgttttccag gctcacgagg acaaattgcg taccttcgat
    14641 acgacagaga tactgatccg gtttgagttt ttcacgaaag cgattgacca cttccagcaa
    14701 tgcctgatcg gcccacgcat agccaaggct atcaatcaca tcctgaatat ggtcaacacc
    14761 gatgagatac accaccggag agacggcttt gtcgaccagg tcatcgaggt aattgtgcag
    14821 gttattgcga tttggcagac cggtcatcgg atcaaattgg atgagttgtt caatatgctg
    14881 acggcttttt tcctgttcca gcgccagcgc ggccatatgc tggctgatat ctgccacgcg
    14941 ttcgataaag gcgctggttt ctgctcctga cgaggtttta atttgcagga tccccgcagg
    15001 cgcaccatca cgctgacgaa tggtcgctga ccagctttgc gcattttgaa cttctgcacc
    15061 gtgcgaagat gacgcccagt gtatcggcat cccgttgcgc agtgcgaaca gcgaaacatg
    15121 cgattcgttg agaacagatt cgatattacg acaaatgatt tcccccattt catgaaatgg
    15181 cgggctgctg cacatggcgg cgagaatatt gccttcaagc tggcggatct gccgttcttc
    15241 ggtgatatcc gagaaagtca ttaccaggtt ctgcagatgc gcgagcacgt cataaaccgg
    15301 gctgatagag gctttaatcc agattttttc accggtgcgc gtcaacagca gaaattcgtc
    15361 ctgatcgcgg gcggttttcc atagcaactg ttgtaaacga atgcggttat cggcagggaa
    15421 ttcaggaatg ttcaggagtg tatcgggctg cataccgctg gcttcgctaa tgcagtaacc
    15481 aaacatttcg gtaaatgcgc gattgcactg cacaatatgg cgttccggat cgaggacaat
    15541 caccggtcgg tcgagatggt caacggcaat aatcaattgt ctggtctgtt ctttttgcgc
    15601 catttctacg ctggcatccc gtaccagcgc caggtaataa actttcccct cggcgctcac
    15661 tttcgatagc gcaaaacggg tccagatttt actgccgtct tttttctcca gctgcagctc
    15721 ccgactcatc ccctcaacac gcgctttacc gccttcacgg ttatgacgaa tgtattcagg
    15781 atgcgcagga cgcaaatccc gcggaatcag catatcaatg ttattgccaa tgacttcttc
    15841 acgtttgtat ccccagagct tctctgcggc ggggttgaaa aacatcactt catcattttc
    15901 gttaattaac accgcaccca tcatattttg ctcaagggcg gggaaaaaaa tgccatcggc
    15961 ggcattatcc gcatcggtta gcttcatgat tacctctgca tccgggcgca tctaaagact
    16021 ggctttccag agttcaacac ggtttctacc tcgtcttttg gcgatataca gagcttcatc
    16081 ggctatttga atgaggcgct catagtcagg atgaccatta aacatggcgg caccgatgga
    16141 aagtgagagg gcaatatctt cgccgtttgc ggctttcagt ttggttttct ccacccgact
    16201 gcgaatacgt tctgcggtac gtaacgtttc gttttcagaa gcttcagtca aaacaatgat
    16261 aaattcatcg cccccgtaac ggaaaacata atcactactg cggacgttgt cataaaaggc
    16321 ctgagagact ttacgcagaa tttcatcgcc agtgttatgg ccccacgtat cgttgatctc
    16381 tttgaattta tcaacgtcaa taatcagcac tgacagcggt gtaccggtcc ggttggcatg
    16441 ggcaatttcg cgtttgaaga tagtcggcag gaaacggcgg ttaagtaatt tcgtcagtac
    16501 atccataccg acttcgtggc gcgatacttc ttcaaacaat tcacgcagca aggtaataat
    16561 ttgcgataca gtatttctta tctgtaataa aaatttcacc cgcagacttc tgttattcaa
    16621 attcctggtg ttacgcatgg tttgattgaa aataccgtcg aaatcctgaa tcagacgaga
    16681 gatatggccc acttcagcaa tgccgctgaa ataatgtcga cctttatggt taaaccacag
    16741 gccaaaatca gcctggctta aaggcaaact actgcctaaa tcagaatcca gcaggatttt
    16801 atagatgata tctatttccc atgaaagtat tgaggctatt tgccgttctt tttcttcttc
    16861 ggcgttttcc agtaacgaga agatacgata gttttcatct tcttttgagg cactactgtc
    16921 actaaaggta aacgcgcggg tcatcacttc catagcgata tcaatactgt taatcgagaa
    16981 atggtagacc tgaagttttt ctgcggcgga ataattcgaa gagaagatta ccggatagag
    17041 gatttttttc agcacccgaa accccatctc gacaatttct accggaattc ctatgcgggc
    17101 atgcacttcc gcgacggtat gctggatttg tattagcctt tcgacatcgt caacctgggc
    17161 agaaagcacg ttaataatcc agcgttccat cgcacttttc aactgccgct caacttgttc
    17221 attactcaag aattcttcgg catgcgggtc gatacggaca attcgataaa actcaatact
    17281 cagataatga gcatgcgcaa cggcaatttc cgcggcttta gcacgaatgg gcggatctgc
    17341 ctgttcgaca agtccggtcc actcatcttt cattctttta aaatacatct ccataattca
    17401 cacccttata aggctgggaa atcagacgga atcaaaacga aacgcaacgt gcgagatcga
    17461 ctaactgcac catattctcc tgaaatatga agatatactg aaaagaaata agcgacttag
    17521 gacagtttca atctacgcta ctgttcttca gaagagtata gcccatcgta attatttttc
    17581 ggtgacagcg aatatcgtat gttttttcat attcatacat ttttattagg ggatttatgg
    17641 ctgtttaact aagtgtggtt aattgcatgg ttaatttgac tttagtaagc atgattagta
    17701 gtgggatagt tcaagagggt aacaagccgg tgggtaatgt accggcttgt tacaaagtaa
    17761 gaatgggagt ttaactgccc caacgacttt gcagatagct gaccgcttgt tgagtctgcg
    17821 gtttattcag atagtcctca cggaacaaga tggtgccgct aatttctggt accgcatcgt
    17881 taagatcgag ctgctttttc agttccggta cgccgccgtt aatcatccag tctggctcta
    17941 tctttgaagg ttcacccact ttatagaagg cgataccgat atacaggcgg gtcctggtcg
    18001 gtttaacgac atccgcccac cattttgcca acacgtcata acgcgcggca ctccgtgaga
    18061 acggccagta aatttgggga gcaatgtaat ccagcaatcc ttgttccacc catcgacgag
    18121 tgtcagcgta ggattcgtca taggctgccg cgcctcgggt atcggagccg agcggatcgt
    18181 gtgatcggtt acgccacacg cctgccgggc taacaccaaa ttcgactccc ggcttaatgc
    18241 ttttaatggt gtgcgatact tttgcaatta actgctgagt attgttgcgc cgccagtctg
    18301 cttttgacgc aaatgcgcct ccgtatttac ggtacgtttc gttatcattt agccgtgaac
    18361 ccggtgactc agtatagaaa tagtcgtcaa actgcacgcc atctaccgga tagcgggaaa
    18421 ccacttctgc gactattgat gtgatccagt cctgaacctc agggatgccc gggtcgagga
    18481 caaagcgatc gccggacgtt ctgatccagt cgcggtgttg cacatagacg ctcgccggtt
    18541 gttgagacag ggtgctattc agttccctga tagtaccggg cttcgtatta accgatacgc
    18601 gataggggtt aaaccaggcg tgtactttca tcccacgctt gtgggcttcg tcgagcataa
    18661 attgcagcgg atcgtaaccc ggattttcac caatcttacc ggtcataaga tcggaccacg
    18721 gcaaaatttt cgatggccac agggcggtac cgtccggctt gacctggaaa aagaccgtgt
    18781 ttatgccgag acgttgcaga tgatccagtt tgtcgatcat cgcttgttgt tgtacacggg
    18841 cccggctggt ggggttacta atgttaaccg aggaaaccgg tggccagtcg aggcgagaca
    18901 ccgtggccag ccagatgcca cgcatcggtt gtgacgattg ttgcgtcgtg gctggtggct
    18961 ttgaacctgc tggtggtgtc accatggact ctggaggcgt gcttttacaa ctacacagta
    19021 aaagtgccag tgcaaccagt atcgctggtc ttctaatcgc taatttctcg tttcgggagc
    19081 agatatccat atgtgctggt ttccggtaaa cagatgtgcg ctcattctcg tacttatccc
    19141 ggttaagtca atacgacagc aagcacgaaa aagggagcga tgaattatcg ctcccttgtc
    19201 ttataaccat tcagacatgg ttagtgtttc ttgtcattca tcacaatata gtgtggtgaa
    19261 cgtgcacgcg ggtgcaggaa gaagtgacct tttggtgcgt tcttactgtt gattggctcc
    19321 agagtgacgc cggtattcgc tttgccttta cgatcatgaa cagcatagag aataaagggc
    19381 agggcaagta ccaccaggaa actaacgacc agtaattcaa gatacatatc ggtagagtca
    19441 ccctggatat tatccggcgg caggaaggaa acaataaacg ccataattga agtcagcaga
    19501 ccgacaatag ccacgaccag tttcacccct ttaccaccag ggatattaaa tgtgcgtttt
    19561 aagtcaggat gtttaagaac caacacaatg tagccaataa acagcatgaa ataagcacac
    19621 agataaatca ccaccgtcag cgccagtgcg atcaggaagg acatgttgtt accgccaccg
    19681 gtattggtga ggatgatcaa cgcgatagaa gtaatcacca gctgcgaaat gaccagcgtt
    19741 accggtacgc catttttgtt cattttagcg aatgccgctg gcagcaggtt tttctgcgct
    19801 gtgacataca tcccgcgaga aggaccaaca atccaggagg cgatttccgc cagaacaccc
    19861 agcaacagca gtgcggagat cacgcgaacc gtccactcaa tttccggtgc cacatgagac
    19921 atcagaacgg taaaggtttg cattacccct gcggagaggt tgatttcatt acccggaatg
    19981 accatcgcaa tagacaaacc accaacagag cttaagcaga ttgccgccac catcagcagt
    20041 aacatagcca gtggatagtc gcgccctggg ttgctcattt cattgacgtg ggttgcggat
    20101 gcttctacgc ccatataact caaaatgaag gcaacaaata ctaccagggt gcccacttta
    20161 gagaagtcag ggaagaaggt cttcgaatcc atttcgatag caacgggggc accggagtgc
    20221 agataaatag ccgctaatgc gatcaaaata aatgcaggta acaggatacc ggcgaagaag
    20281 ccaactttag caattcgcgc cgtgtatttc gtgccaccaa actgcgttaa tgccagcgcc
    20341 caaagaatga tgagtgctgc aatagttttg gtaatggggt cttcattcag cgctggccat
    20401 ttcaggatgt aggagagtgc ccctaacacg aaatagagca tcggaataaa accaatggcg
    20461 atttgcagat agccaaatga gatcgctgca aatccccatc tcggccccag agtatttgat
    20521 acccaggcga agacaccacc ttcttcccag ccgtcgacgg tggccatttc cgcagcacaa
    20581 agtcccacgg gaataaacca taaaatcccg cctaatagca ggaagaagac taatgaaaag
    20641 cccgatgttg cgaaggtagg gtattcataa acagccatta ccatcgatgc cgttatggca
    20701 aagaatccga gtaatgtgag ctgcttagct ttacctgtct gtactgatgt agccatatta
    20761 tccccctaaa acggtattcc tgtcggaacc gcacctgttt tgttctgaag cgtattcaga
    20821 acaatatttt ccgttgctaa tgccagtgaa cagactttgg aaattgtccc gaaacgggtt
    20881 cgtttcggga caccgttacc gttaaacgtt atcaggtatg tttaaagctg ttctgctggg
    20941 caataccctg cagtttcggg tgatcgctga gatatttcag ggaggctttg tagtcttcca
    21001 gcaacagttc agcaaagtcc atttcgaagc cgcgacgaca cataatgcgc atcaccacga
    21061 tgtcggtggc ttcaccgccg agagtgaagg ccggaacctg ccagccgcgc agacgcagac
    21121 gttcagagag gtcgtacagg gtgtatcccg gatcttcacc ctctttcagt ttgaagcaaa
    21181 ccgccggaat gccttcgtcc gggcgaccgg tacagatgaa ctcatacggc cccagtttgg
    21241 cgatttcatc cgccagataa gcggcaacct ggtaagaggc gttctgtact ttggtgtagc
    21301 cttcacgacc gaggcgcagg aattcatagt actgtgcaat tacctgacct gccgggcggg
    21361 agaagttgat ggcgaaagta ccaatctgac caccgaggta gtcaacgttg aacaccagtt
    21421 cctgcggcag cgcttcttcg tcacgccaga taacccagcc gcagcccagc ggagccagac
    21481 cgaatttgtg gcctgaagca ctgatcgatt tcacacgcgg caggcggaag tcccagacga
    21541 tatccggggc gacgaacggt gccaggaagc caccgctggc ggcgtcgatg tgcatgtcga
    21601 tgtcgatacc ggtgtcggcc tggaatttat ccagcgcatc gtgcagcggt tgcgggaact
    21661 catagttacc ggtgtaggtc acgccgaaag tcggcaccac gccgatggta ttttcgtcgc
    21721 aggcttcaat catgcgtttc ggatccataa acaactgacc ggggcgcata gggatctcac
    21781 gcagctccac atcccagtag cgggcgaatt tatgccagca gatttgcacc ggaccgcaca
    21841 ccaggtttgg tttatccgtt ggtttgcctg cagcttccat acgcttgcgc caacgccatt
    21901 tcatcgccat cccgccgagc atacaggcct cggaagaacc aatggtgttg gtgccaacgg
    21961 cctgaccatt tttcggcgca ggcgcatgcc acagatcggc aaccatattt acgcaacgca
    22021 ggtcgatggc tgcggattgc ggatattctt ctttgtcgat ccagtttttg ttaatggata
    22081 aatccatcaa cttgtggacg ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct
    22141 gacgagcgtt gccatcaaga tataattcgt cattgataat ctgaaatgcg acatcgtcgc
    22201 gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac ttcgcaccaa
    22261 aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc attttaaact
    22321 ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta agcaagtgc
//
LOCUS       DAPGRN010000079        22349 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPGRN010000079 DAPGRN010000000
VERSION     DAPGRN010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.079"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            255..1373
                     /locus_tag="RHW33_003145"
     CDS             255..1373
                     /locus_tag="RHW33_003145"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV1646624.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHKEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1439..1687
                     /locus_tag="RHW33_003146"
     CDS             1439..1687
                     /locus_tag="RHW33_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV1646625.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     IDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1752..2120
                     /locus_tag="RHW33_003147"
     CDS             1752..2120
                     /locus_tag="RHW33_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV1646626.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLCDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2214..2867
                     /gene="nfsB"
                     /locus_tag="RHW33_003148"
     CDS             2214..2867
                     /gene="nfsB"
                     /locus_tag="RHW33_003148"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV1646627.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3125..4372
                     /gene="ybdG"
                     /locus_tag="RHW33_003149"
     CDS             3125..4372
                     /gene="ybdG"
                     /locus_tag="RHW33_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV1646628.1"
                     /translation="MQDLISQVEDLAGIEIDNTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4440..5816)
                     /gene="pheP"
                     /locus_tag="RHW33_003150"
     CDS             complement(4440..5816)
                     /gene="pheP"
                     /locus_tag="RHW33_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV1646629.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5918..9061)
                     /gene="cusA"
                     /locus_tag="RHW33_003151"
     CDS             complement(5918..9061)
                     /gene="cusA"
                     /locus_tag="RHW33_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV1646630.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLGEVKNALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHRPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9073..10296)
                     /gene="cusB"
                     /locus_tag="RHW33_003152"
     CDS             complement(9073..10296)
                     /gene="cusB"
                     /locus_tag="RHW33_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV1646631.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10312..10644)
                     /gene="cusF"
                     /locus_tag="RHW33_003153"
     CDS             complement(10312..10644)
                     /gene="cusF"
                     /locus_tag="RHW33_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV1646632.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10668..12050)
                     /gene="cusC"
                     /locus_tag="RHW33_003154"
     CDS             complement(10668..12050)
                     /gene="cusC"
                     /locus_tag="RHW33_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV1646633.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALENNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12207..12890
                     /gene="cusR"
                     /locus_tag="RHW33_003155"
     CDS             12207..12890
                     /gene="cusR"
                     /locus_tag="RHW33_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV1646634.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12880..14322
                     /gene="cusS"
                     /locus_tag="RHW33_003156"
     CDS             12880..14322
                     /gene="cusS"
                     /locus_tag="RHW33_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV1646635.1"
                     /translation="MVNKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEIGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVDHQVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFIITLPA"
     gene            14472..16709
                     /gene="nrfB"
                     /locus_tag="RHW33_003157"
     CDS             14472..16709
                     /gene="nrfB"
                     /locus_tag="RHW33_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV1646636.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDVLIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            16696..19668
                     /gene="nfrA"
                     /locus_tag="RHW33_003158"
     CDS             16696..19668
                     /gene="nfrA"
                     /locus_tag="RHW33_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV1646637.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALTYRSEKARLQRYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGSTLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARISQTLMARLTSLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYTTALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            19669..20559
                     /locus_tag="RHW33_003159"
     CDS             19669..20559
                     /locus_tag="RHW33_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV1646638.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEHVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            20742..21503
                     /gene="envY"
                     /locus_tag="RHW33_003160"
     CDS             20742..21503
                     /gene="envY"
                     /locus_tag="RHW33_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HDV1646639.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(21549..21625)
                     /locus_tag="RHW33_003161"
     tRNA            complement(21549..21625)
                     /locus_tag="RHW33_003161"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21589..21591),aa:Arg,seq:tct)
     gene            21868..>22349
                     /gene="fimZ"
                     /locus_tag="RHW33_003162"
     CDS             21868..>22349
                     /gene="fimZ"
                     /locus_tag="RHW33_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV1646640.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     L"
BASE COUNT         5444 a         5959 c         5590 g         5356 t
ORIGIN      
        1 agtcttgttt cggacttctc actcctcatt tgatcaactg aagctgaacc cgttttttct
       61 gcatgcctca atcatcaaaa ttcttcgcca ccagccagct ctgccataac ttgtcacatt
      121 gaaaaagtag ccaaattgct tcaggcacca tcagttcacc atcaccaaaa aaagaaacat
      181 tccagaactt ccgcatctcg cccagccgct ccgacttcta tactgaatag aaaacgccaa
      241 cataagagaa acctatgcca ttacccgatt ttcatgtttc tgaacctttt accctcggta
      301 ttgaactgga aatgcaggtg gttaatccgc cgggctatga cttaagccag gactcttcaa
      361 tgctgattga cgcggttaaa aataagatca cggccggaga ggtaaagcac gatatcaccg
      421 aaagtatgct ggagctggcg acggatgttt gccgtgatat caaccaggct gccgggcaat
      481 tttcagcgat gcagaaagtc gtattgcagg cagccgcaga ccatcatctg gaaatttgcg
      541 gcggtggcac gcacccgttt cagaaatggc agcgtcagga ggtatgcgac aacgaacgct
      601 atcaacgaac gctggaaaac tttggctatc tcatccagca ggcgaccgtt tttggtcagc
      661 atgtccatgt tggctgtgcc agtggcgatg acgccattta tttgctgcac ggcttgtcac
      721 ggtttgtgcc gcactttatc gccctttccg ccgcgtcgcc gtatatgcag ggaacggata
      781 cgcgttttgc ctcctcacga ccaaatattt tttccgcctt tcctgataat ggcccgatgc
      841 cgtgggtcag taactggcaa caatttgaag ccctgtttcg ctgcctgagt tacaccacga
      901 tgatcgacag cattaaagat ctgcactggg atattcgccc cagtcctcat tttggcacgg
      961 tggaggttcg ggtgatggat accccgttaa cccttagcca cgcggtaaat atggcgggat
     1021 taattcaggc caccgcccac tggttactga cagaacgccc gttcaaacat aaggagaaag
     1081 attacctgct gtataaattc aaccgtttcc aggcctgtcg ctatgggctg gaaggcgtca
     1141 ttaccgatcc gcacactggc gatcgtcgac cgctaacgga agataccttg cgattgctgg
     1201 aaaaaatcgc cccttccgca cataaaattg gcgcatcgag cgcaattgaa gcactgcatc
     1261 gccaggtcgt cagcggtctg aatgaagcgc agctgatgcg cgatttcgtc gccgatggcg
     1321 gctcgctgat tgggctggtg aaaaagcatt gtgagatctg ggccggtgac taagccggga
     1381 attgccttac agaagaatat ccctgacaat ggtgttttaa cttacattta acaatgttat
     1441 gaaacaccct ttagaaacct tgaccaccgc agcaggcatt ttgctgatgg ctttcctctc
     1501 ttgcctgctg cttcccgccc ccgcattggg gctgacgctg gcacaaaaac tggtgaccac
     1561 gttccatctg atagatctta gtcagcttta cactttattg ttttgtctgt ggtttttagt
     1621 gctgggcgct attgagtatt ttgttctgcg ctttatctgg cgacgctggt tctcgctggc
     1681 ggattaaacg tcgattatcg ccgtacttcg cggcatactt tgcttattct ccttcgccta
     1741 aagtaacgtt tatggataag caatcactgc acgaaacggc gaaacgcctg gcccttgagt
     1801 taccctttgt cgagctttgc tggccttttg gcccggagtt cgatgttttt aaaattggcg
     1861 gcaagatttt tatgctgtcg tcggagctgc gcggcgtccc ctttatcaat ctgaagtccg
     1921 atccacaaaa atccctgtta aatcagcaaa tatacccaag cattaagcca gggtatcaca
     1981 tgaataaaaa gcactggatt tcggtgtatc ccggcgagga aatctccgaa gcgttacttt
     2041 gcgatctgat caacgattcg tggaatctgg tggttgatgg tctggctaaa cgcgatcaaa
     2101 aaagagtgcg tccaggctaa agcggaaatc tatagcgcat ttttctcgct taccatttct
     2161 cgttgaacct tgtaatctgc tggcacgcaa aattacttac acatggagtc tttatggata
     2221 tcatttctgt cgccttaaag cgtcattcca ctaaggcatt tgatgccagc aaaaaactta
     2281 ccccggaaca ggccgagcag atcaaaactc tcctgcaata cagcccatcc agcaccaact
     2341 cccagccgtg gcattttatt gttgccagca cggaagaagg taaagcgcgt gttgccaaat
     2401 ccgctgccgg taattatgtg ttcaacgaac gtaaaatact tgatgcctcg cacgtcgtgg
     2461 tgttctgtgc aaaaaccgcg atggacgatg cctggctgaa gctggttgtt gaccaggaag
     2521 atgctgatgg ccgctttgcc acgccggaag cgaaagccgc gaacgataaa ggtcgcaagt
     2581 tcttcgccga tatgcaccgt aaagatctgc atgatgatgc agagtggatg gcaaaacagg
     2641 tttatctcaa cgtcggtaat ttcctgctcg gcgtggcggc tctgggtctg gacgcggtac
     2701 ccatcgaagg ttttgacgcc gccatcctcg atgcagaatt tggtctgaaa gagaaaggct
     2761 acaccagtct ggtggttgtt ccggtaggtc atcacagcgt tgaagatttt aacgctacgc
     2821 tgccgaaatc tcgtctgccg caaaacatca ccttaaccga agtgtaattc tcctgacagc
     2881 cgggacccca tcccggctca gattgctgac aaagtgcgct ttgtttatgc cggatgcggc
     2941 gtgaccgcct tatcctgcct acaagttcgt gccaattcat tcaggtaggc atgataagcg
     3001 tagcgcatca ggcaattttg tatttgccat cagccctcgc aggaaatccc ccctcagatt
     3061 ctcctgattt gcataaccct gtttcagccg tcatcatagg ctgctgttgt ataaaggaga
     3121 cgttatgcag gatttaatat cccaggttga agatttagcg ggtattgaga tcgataacac
     3181 cacctcgatg gtgatgattt tcggtattat ttttctgacc gccgtcgtgg tgcatattat
     3241 tttgcattgg gtggtactgc ggaccttcga aaaacgtgcc atcgccagtt cacggctttg
     3301 gttgcaaatc attacccaga ataaactctt ccaccgttta gcttttaccc tgcaggggat
     3361 tatcgtcaat attcaggcgg tattctggct gcaaaaaggc accgaagcgg cagatattct
     3421 gactacctgc gcgcagttgt ggatcatgat gtatgcgctg ctttcagtct tctcgttgct
     3481 ggatgttatt ttgaatctgg cgcagaaatt cccggcagca tctcagttac cgctgaaagg
     3541 gatatttcag gggattaaac tgatcggcgc gattctggtc ggcattttga tgatctcgct
     3601 gctgattggt cagtcgccag cgattctgat aagcggtctt ggtgcaatgg ctgccgtgct
     3661 gatgttggta tttaaagatc cgattcttgg tctggtggca ggtattcagc tttccgcgaa
     3721 cgatatgctg aaactgggcg actggctgga gatgccgaaa tacggcgcgg atggcgcggt
     3781 gatcgatatt gggttaacca ccgtcaaagt gcgtaactgg gacaatacca ttaccactat
     3841 tcccacctgg tctctggttt ctgactcctt taaaaactgg agcgggatgt cagcatccgg
     3901 cgggcgacgt attaagcgca gtatcagtat tgatgtcacc agtattcgtt ttcttgatga
     3961 agacgaaatg caacgtctga ataaagcgca tttgttaaag ccttatttaa ccagccgcca
     4021 tcaggaaatt aatgagtgga atcgccagca aggttctacg gagtcggtat taaacctgcg
     4081 ccgaatgacc aatattggaa cctttcgtgc ctatctgaac gaatatctgc gtaaccatcc
     4141 gcggattcgt aaagatatga ccttaatggt acgccaactg gctccaggtg ataacggttt
     4201 accgctcgag atctatgcgt ttaccaacac cgtggtgtgg ctggaatatg aaagcattca
     4261 ggctgatata ttcgatcaca tatttgcgat tgtcgaagag tttggtctgc gacttcatca
     4321 gtcgccaacc ggcaatgata ttcgctctct ggcgggtgca tttaagcagt aattaaaaaa
     4381 accgctctca tcgaatggat gagagcggac atcgttttgt cggatgtagc gtgaatgcct
     4441 tatttccgac gcagcgtttt aaatgccaca aacaggaata caatccacac cggcagcagg
     4501 atcgctgaca agcgcatatc atccatcgtg cacatcagca gcaaaatcat gccgaggaag
     4561 gcaatgcaga gatagtttcc gaacggatag agcagcgcct taaactgtgt ttcacgcccc
     4621 tgacgtcgca tcgctgcacg aaaacgcaga tgcgccagac agatcataat ccagttcaac
     4681 agcagcgttg ctaccaccag cgccatcagc agaccaaacg ctttttgcgg cagcagatag
     4741 ttgattaaca ccaccagcga agtgatcgct ccggaaagca tcagcgagtt aatcggcacg
     4801 ccgcgacggc tgacgcgagt caaaaacttc ggcgcattac cctgtacaga aaggccaaac
     4861 agcatgcggc tgttagagta aaccccgctg ttatacactg acagcgatgc taccagaatg
     4921 acgaagttca gcgcagaagc taccacgttg ctgtcgagat tatggaaaat catcacaaac
     4981 gggctactgt tggatttcac ttccacccac ggatagagcg ccagtaaaac caccagtgaa
     5041 ccgatgtaaa acagcaggat gcgatacacc acctgattta ccgcttttgg aatgcttttt
     5101 tccggatcgc gcgcttcagc ggcagtaatc ccaatcagct ccagaccgcc gaaggagaac
     5161 ataattaccg ccagcgacaa aatcagccca ttccagccgg tggcgaagaa accaccgtag
     5221 cgccagaggt tgtcgatact ggctttctcg ccgccgtgac cagaaaacag cagccacagg
     5281 ccaaagccga tcataccgat gattgccagc actttaatca gcgcaaacca gaactcggtt
     5341 tcgccatata agcgcacgtt caccaggtta acggcgttga tgataataaa gaaggcggca
     5401 gcccaaatcc acgttggaac atccgggaac cagtactgca tatagatgcc cgcagcggtc
     5461 agctctgcca ttcccaccag cacgaacatc acccagtaat tccagccaga gaggaagccc
     5521 gcgaacggtc cccagtattt ataggcaaag tgggcaaatg aaccggatac cggctcctca
     5581 accaccattt cgccaagctg gcgcataatc aggaaagcga tgatcccggc gacgccgtag
     5641 cccagcaata cagccggacc cgccatctga atcgccgggc caatgccaag aaacagacca
     5701 gtaccaattg cgccacccag cgcaatcagt tgaatatgac ggttatgtaa tccgcgatga
     5761 agcgtcggct cttgattcga cgcagtatct tccgatacgg ttgacgcgtt tttcacgcat
     5821 ttcccctgtg tgtctttttt gttgaggggc accttttaac atttagtgcc catcgtcgca
     5881 agacacaatc cacacggtta aacggggtat cctgctttta tttccgtacc cgatgtcggt
     5941 gcagccacat cagcttatac gccgccggga taataaacag cgacagcaaa ggcgcggtga
     6001 tcatgccgcc aatcatcggc gcggcaatcc ggctcatcac ctctgaccct gctcctgttc
     6061 cccacagaat cggcaacaga ccagcgataa tcaccgccac cgtcatcgct ttcgggcgca
     6121 cgcgcaggac cgcgccgtga tacaacgcct catccagctt ctgctcgctg aatgtttgcg
     6181 gattattcaa cgacggctcg gcctctatgg cgtgacgtaa atacatcagc atcaccacgc
     6241 caaattcggc ggcgaccccg gcaagggcaa taaaaccggt gcccgtcgcc acagaaagat
     6301 gaaagcccat ccaccacagc aaccagatgc cgcccaccag cgcaaacggt acgctgctga
     6361 taatcagcaa cgcttcgccc acccgacgga atgccagata caacagcacg aagatgatca
     6421 tcagtgtcat cggcaccatg agtttcagct tatggttggc gcgctccagt agctcgaact
     6481 gcccggagaa tgccacgctg gtgcccggtt ttaactgcac tttttcagct atcgcttttt
     6541 gcaaatcgtg aaccaccgac accatgtcac gatcgcgggc atcgatataa atccagctcg
     6601 tcgggcgcgc attctcggtt ttcagcattg acggtccggt agagacttta acgtcggcca
     6661 cgtctgccag ggtgatttgc tgcttcatcg gcgtcaggat cggcaactgg cgcagagcct
     6721 gcggactatc gcgccagctt tgcggataac gcagattaat tggataacgg gcaatccctt
     6781 ccaccgtttc gccaaccatc gccccgccca ccgcagaagt cacaaacaac tgcacatccg
     6841 ccaccgtcat accgtaacgt gcggcttttt cacggttaat ctcaacgttg atatagcgcc
     6901 caccttccag acgctcggca agcgcagaag ccacgcctgg caccgttcgt gccacctctt
     6961 caatttgctc cgccatcgcg tcgatatccg ccagcacagt gccggaaact tttatgccga
     7021 tagggctttt aatgccggtt gagagcatat cgatacggtt acgaattggc ggcacccaca
     7081 gattcgccag ccccggcagc cgcacggtgt tatccagttc ctcaatgatt ttgtccatcg
     7141 tcatgcctgg ccgccactga tcctgtggct taagctggat ggtcgtttcc accatctcca
     7201 gcggagcaga gtcggtggcg gtttccgctt ttccggtttt gccaaatacc cgcgccactt
     7261 caggtacgct cataatcagc ttgtcggttt tttgcagcat actcgccgcc tccgctgcgg
     7321 aaatccccgg caacgtcgat ggcatataca acaagtcgcc ttcattgatc tgtggtaaaa
     7381 attccccgcc aactttattg agcggccaga gaaccgtcag caccgaaagc gccgccacca
     7441 gcagcgtggt tttcggcctg tgcagtactt tcagcaacag cggatgataa acacgaatca
     7501 aaaagcgatt gagcgggtta ctactttctg gcggaatttt gccacggatc cagtaaccca
     7561 tcaggatagg gatcactacg atcgccagca gagccgcacc cgccatcgca taggttttag
     7621 tgaacgccag cgggccaaac agacgccctt cctgcccttc cagggtgaag atcgggataa
     7681 acgacaacgt gataatcagc agactgataa acagcgccgg ccccacttca acagacgcat
     7741 cggtgatcac ctgccagcgc gttttattat ccagcgtggc gtcaggatgc tggtgctgcc
     7801 actcttccag ccgtttatgt gcattctcga tcatgacgat agcggcatcg accatcgccc
     7861 cgacggcaat cgcaatgcca cccagcgaca taatattggc attcagcccc tggaagtgca
     7921 tgacaataaa agcaatacac aaccccagcg gcaacgaaat aatcgccacc agcgccgagc
     7981 gcacatgcca gagaaacagc gcacagacta ccgccaccac aataaactct tccagcaact
     8041 tgccgctgag gttgtcgatg gcccggtcaa tgagctggct gcgatcgtat gttgtaacta
     8101 tctccacgcc ttccggcaga ctacttttca gcgtttccag tttgtccttc acggcggcga
     8161 tcacttctcg ggcgtttttg ccggagcgca ggatcaccac cccgcccgca acttcgcctt
     8221 cgccgttcag ttcggcaatg ccccggcgca tctccgggcc aacctggacc ttcgcaacat
     8281 cacgcagata aacgggcacg ccattttcac tggcttttaa aacgatgtga ttaaagtcgt
     8341 cgagcgtttg cagatagccg ctggcgcgca ccatatattc cgcttccgcc agttcgatcg
     8401 acgaaccgcc cgcttcctgg tttgaagcat ccagcgcatt tttcacttcg ccgagactga
     8461 tgccatactg cgccaggcgc tgaggatcga taaccacctg atactctttc accacaccgc
     8521 ccaccgacgc cacttccgca acgtcaggga tggtttttag ctcatatttg agaaaccagt
     8581 cctgcaatga gcgtaaatcg gcaagatcgt gcttaccgct gcgatccacc agtgcatatt
     8641 catagatcca gccaacaccc gtggcatctg gccccagctc ggcgctgact cccgcaggca
     8701 gcttaccctg tacctggttg aggtactcca gcacccgcga gcgcgcccag tacggatcgg
     8761 tgccatcttc gaaaatgaca tacacataag agtcgccaaa ctgcgagaaa ccgcgcacag
     8821 tcttcgcgcc aggtaccgac aacatggtgg tagttagcgg ataagtcacc tgattttcaa
     8881 cgatttgcgg tgcctgcccg ggatagctgg ttttaataat cacctgcaca tcggagagat
     8941 ccggcagcgc atccactggc gtgttaatga tggtccaggt accccagatg ctcagaaaca
     9001 acgcacccat cagcaccaaa aaacggttcg ccaccgagcg acgaataatc cattcaatca
     9061 ttggttattc cctcaatgcg catgggtagc actttcagag cgcatccgct ccagtgcgcc
     9121 agaaatattg gcttcggaat caatcaggaa caggccgctg gaaaccacct tttcaccttc
     9181 cgccagacca gagcgtaatg cggtgacacc ttgcgatgcc tggaaaacgg caacgcgttt
     9241 tggtacaaag cgcccgtcgg catcaacggt aatcacccgc tgttcgctac cggtatcaat
     9301 cagcgcttgt gacggaatga gcagcatcgg ttcgctggcg gtattgagtt gcaaccaggc
     9361 gttcattccc ggttttagcg cctcgtcggc gttgtcgact tccagacgca gctgcagcgt
     9421 gcgggtcgca gcatccacgc cgggtagcag cgtccatttg cggatggtga gtgttttatc
     9481 cggtcgtgcc ggaacggtga gcgtaaactg cgaggcatct ttcaccagcc aggcgataga
     9541 ctccgggatc gcagcagtga cccacaccgg gtccataccc tgaattttcg ctaccacatt
     9601 atctttggcg atattcattc ccgcgcgcag atcaaacgcg gtgatcacgc catcaatagg
     9661 tgctttgagc gtaaagcgag tctggatttt ttgcgtggcg atcaggcggc gaatatccgc
     9721 ctccggcatt cccgccagtc gcagccgctc aagaatgcct tcagtctggg tcgccgtacc
     9781 gcccgtttcg cgcagcagta aatactcact ctgcgcttcc acccagtcgg gaatggtcag
     9841 gtcgagaagc ggtgtgccct tttgcacttt atcgcccacg gtaagcggat acaccttgtc
     9901 gataaacccg gcagcgcgag cctgcacaat ggcatactga tactcgttgt aactgacatt
     9961 cgccgggaaa ctctgggcaa aagtcagcgg cccgcgcgtg acggtagccg ttttcacccc
    10021 cagattctgc gtctgagtcg ggtcaatgcg cacaccagac gcagaactct cttcatcggc
    10081 atatttcggc accagatcca tatccataaa cggcgattta cctggtttat cgaaccgcgt
    10141 attgggatac atcgggtcgt accagaataa gattttacgt tctgcggtcg acgttttttc
    10201 tgcgggcggt tccgcctttg caaaccaggt aaaacctgcc gcagaaataa taccgcccgc
    10261 aatcatgctg ccgataataa gcgcgatttt tttcatctca ttaaacctgg gttactggct
    10321 gactttaata tcctgtaata aagaaaggtt gccctgctgg acaaaattaa acgccacttt
    10381 gtcgccggtt ttaatttcac tcattttcgt ctgcggggtg atggtaaagc gcatggtcat
    10441 ctccggccag tttacagcag caatcggatc gtgatggatg gtgatttttt tgctttccag
    10501 atcaataccc tttaccacgc cagtggcgct aataacctgt ggttgtgctt cgctcatggt
    10561 ttcatgatga tgttcgttag cctgggcatt aaagccaata acggtaaaca gactgaacat
    10621 tgcgacttgc agtgcttttt tcatttattc tctcctggag ttaaaagtta ttgctgccaa
    10681 ccgccaccaa gtgcggtata taaagaaatt tcgttaacct gacgggcata attcaggtca
    10741 agtaaagttt gtcgggttgc aaataacgaa cgctcggcat ccagcacttc cagataactt
    10801 accgctccgt gctgatataa tgcccgcgcc cgttgcaaag taatttgcag cgatgccaga
    10861 taacgctgct gggcgctaat ttgatcgttc aggctttgac gtaatgccag cgcatctgcc
    10921 acttctttaa aggcgttctg gattttctgt tcataattta ccaccgactg ctgctggcga
    10981 atttcggcga tatccagatt ggcctggttg cgtccggcat taaaaatggg gatctcaatt
    11041 ttaggaataa aattccacat cccgctgctg gcgttaaata atgacgacag atcgctgctg
    11101 gcggttgata ttccgctggt caggctgata gacgggaaaa atgctgcacg tgcagcacca
    11161 atattggcat tagcagccat taacgcgtgt tcagcttcca taatatccgg gcgctgcaat
    11221 aagatttgcg acgacaagcc cgccggtaat ttaacgcttt gcagactgtc gctgtttact
    11281 gtctgcgctt gcggcagctt gccgtagctt cccaataaca gttgcaatgc attattcgcc
    11341 tgcgccagtt ccccctgacg tttagcgatg tcgctgcggg tactttcaat caccccgcga
    11401 gcctgttcca gcgccagaac attgctgcta ccggtcaaca gttgtttttc gacaaacgca
    11461 tatgactgct gataattacg cagcgtttct tcggctattt gcaattgcgc atacgccagc
    11521 tgctgattga aatagctttg cgcgacatta gaaaccagca gaatatgcac cgcgcgctga
    11581 gcttcctcag tggctaaata attttgtcgc tcggcttcgc tcatgttctt taagcgcccg
    11641 aaaaaatcga ggtcaaagct ggcgttaagg ccagtagaaa actcccgcgt cgtggctgaa
    11701 tcgcctttaa gattgccgct ccagctgccg ctgccctcgc cattgagctg tgggtagcgg
    11761 tcggcatcgg tcagacgata ttgcgcccgt gcctcctgca ctttcagcgt cgccatgcgc
    11821 aaatcccggt tattctccag cgcctcgcta atcagcgtct tcacctgatt atcaacaaaa
    11881 aaggtgcgcc agcccgcgtt ctgatagtta tctgctgcgt taaccaggcc gttctggctg
    11941 agtgagaact gctgaggaac gggcattgcc ggacgctgat aatccggtgc cagtgaacaa
    12001 ccggttagcg caagggccac acaaaatggc agaagtttac aaggagacat aggctcataa
    12061 tttctggtga ttttataccg ccaactttac tcgccaggct ctgattttcc ggtgacagga
    12121 aaatgacaaa attgtcattt tgccaataag cgattgccat ctgatcccgc tactctagaa
    12181 ttgcccgggc aacatgcgga ggaaatatga aactgttgat tgtcgaagat gaaaagaaaa
    12241 ccggagaata cttgaccaaa gggttaaccg aagccggttt tgtggtcgat ttggccgaca
    12301 acgggctgaa tggctaccat ctggcgatga ccggtgatta tgatctgata atcctcgata
    12361 ttatgctgcc ggacgtgaac ggctgggata tcgtgcgcat gttgcgctcc gccaataaag
    12421 ggatgccgat tctgttgctt accgcgcttg gcaccattga acatcgcgtc aaggggctgg
    12481 agttgggggc agatgactac ctggtgaaac cattcgcttt tgctgaactg ctggcgcggg
    12541 tgcgcactct cctgcggcgc ggggcggcgg tgattatcga aagtcagttt caggttgccg
    12601 atctgatggt cgatctcgtc agccgcaaag tcacccgcag cggcacgcgc atcactttga
    12661 ccagtaaaga gtttactctg ctggagtttt ttctccgcca tcagggcgaa gtgctgcccc
    12721 gctcgcttat cgcctcgcag gtatgggaca tgaattttga cagcgacact aacgccattg
    12781 atgtggcggt gaagcgactg cgcggcaaaa tcgacaacga ctttgagccg aagctgattc
    12841 agaccgtgcg cggcgtgggc tatatgcttg aggtacctga tggtcaataa gccatttcag
    12901 cgcccgtttt cgctggcaac ccgcctgacc ttttttatca gcctggccac catcgcggcg
    12961 tttttcgcct ttgcatggat catgatccac tcagtaaaag tgcattttgc cgagcaggat
    13021 attaatgatt taaaagagat tagcgccacc cttgaacggg tactaaatca ccctgacgaa
    13081 acgcaagccc gacgcttaat gacgctggaa gatatcgtca gtggttattc caatgtgttg
    13141 atttccctgg cagatagtca gggtaaaacg gtgtatcact cccccggtgc gccggatatc
    13201 cgcgagtttg cgcgtgacgc catacccgat aaagacgcgc ggggcggtga ggtgtatctc
    13261 ctttccggcc cgacgatgat gatgccaggc cacggtcacg ggcatatgga acacagcaac
    13321 tggcggatga ttaacttgcc ggttggcccg ttggtggacg gaaaaccgat ttatacgctc
    13381 tatatcgcgc tttcgatcga ttttcatctt cattacataa atgatttgat gaataaactt
    13441 attatgaccg catcgatcat cagcatcctg atcgtcttta tcgtactgtt ggcggtacat
    13501 aaaggtcacg cgccgatccg cagcgtcagc cgtcaaatcc agaatatcac ctcgaaagat
    13561 ctcgacgtcc gccttgaccc gcagaccgtg cccattgagc tggaacagct ggtactgtcg
    13621 ttcaaccata tgatcgagcg tattgaggat gtctttaccc gccagtccaa tttctcagcg
    13681 gacatcgccc acgaaattcg cacaccaatt acgaatctca taacgcaaac cgaaatcgcc
    13741 ctcagccagt cgcgcagcca gaaggagctg gaagatgtgc tctactctaa tctcgaagag
    13801 ctgacgcgaa tggcgaaaat ggtcagcgat atgctgtttc tcgctcaggc cgataacaac
    13861 cagctaatcc ccgaaaagaa aatgctcaac ctggcggatg aaatcggcaa agtgttcgat
    13921 tttttcgagg ctttagcgga agatcgcgga gtggagttgc ggtttgttgg cgacaaatgt
    13981 caggttgcgg gcgatccgct gatgctgcgt cgggcgttaa gcaacctgct ttctaatgcc
    14041 ctgcgttata cgccacccag tgaggctatt gtagtgcgct gccagacggt cgatcatcag
    14101 gtgcaagtta tcgtcgaaaa ccccggtact cccattgcgc ccgagcactt accgcgattg
    14161 tttgaccgtt tctaccgcgt tgacccttcc cgccagcgaa aaggcgaagg tagcggcatt
    14221 gggctggcga tagtgaaatc gattgttgtc gcacataaag gcacggttgc agtaacgtca
    14281 gatgcgcggg ggacaaggtt tattatcaca ttacccgctt aattattcag caaacggcaa
    14341 cttttataac cagtgtaaaa ataacgtgcc gcaatagtca tacctattaa tggtaaaaag
    14401 ctgtcacaat tcataaaaaa ccttaatata cgccacccta aacataacca gcgttaatgt
    14461 aaggtttttg tgtggactgg cttcttgatg tttttgctac ctggctctac ggcttaaaag
    14521 taatcgcgat aacgttagcg gtcatcatgt tcatcagcgg gctggacgat ttttttattg
    14581 atgtcgtcta ctgggtacgc cgcattaaac gcaagttgag tgtttatcgc cgctacccgc
    14641 gaatgagtta ccgcgaactg tataaaccag atgaaaaacc gttagcgatt atggttccgg
    14701 cgtggaatga aacgggcgtc atcggcaata tggccgagct ggcggcgacc acgctcgact
    14761 acgaaaacta tcatatcttt gttggcacct accccaacga cccggacact cagcgtgatg
    14821 ttgacgaagt gtgcgctcgc ttcccgaatg tgcataaggt agtctgcgcg cgtcctggcc
    14881 ccaccagcaa agccgactgt ctgaacaacg tgctggacgc catcacccaa tttgagcgta
    14941 gcgccaattt cgcttttgct ggttttattc tgcatgacgc cgaagatgtg atttcaccga
    15001 tggaattgcg tctgttcaac tatctggtcg agcgtaaaga tctgattcag atcccggtgt
    15061 atccgtttga acgcgaatgg acgcacttca ccagcatgac ttacattgat gagttttcag
    15121 agctgcatgg caaagatgtt ccggtgcgtg aagccctcgc cggacaagtg cccagcgcag
    15181 gcgtcggcac ctgtttcagc cgccgcgccg tgaccgcact gttagctgac ggtgacggta
    15241 ttgctttcga cgtgcagagt cttactgaag attacgacat tggcttccgc ctgaaagaaa
    15301 aaggtatgac ggaaattttt gtccgttttc cggtggtgga cgaagccaaa gaacgcgagc
    15361 agcgtaaatt tttacagcac gcacggacgt caaacatgat ctgcgtgcgc gaatatttcc
    15421 ccgatacctt ttcgactgcg gttcgacaaa aatcccgctg gatcatcggc attgttttcc
    15481 agggctttaa aacccataaa tggacctcca gcctgacgct gaactacttt ctctggcgcg
    15541 accgcaaagg ggcaatcagt aactttgtca gcttcctcgc gatgctggtg atgatccagc
    15601 ttttgctgtt gctggcgtat gaaagtttgt ggcccgatgc ctggcatttc ctttctattt
    15661 tcagcggcag cgcatggtta atgaccctgc tgtggctaaa ctttggtttg atggttaacc
    15721 gcatcgtgca gcgggtgatt ttcgttactg gctactacgg cctgacgcag gggctgcttt
    15781 ccgtcctgcg tcttttctgg ggcaacctga ttaacttcat ggccaactgg cgcgcgttaa
    15841 aacaggtact tcaacacggc gatccacgtc gcgtggcgtg ggataaaaca acgcatgact
    15901 tccccagcgt cactggcgat acccgctcgt tgcgcccgtt aggtcaaatt ctgctggaaa
    15961 atcaggtcat cactgaagaa caactcgata cagcactgcg taatcgcgtc gaaggtctac
    16021 gcctgggcgg ttcaatgctg atgcaggggc tgattagcgc cgagcaactg gcacaggcgt
    16081 tggcagagca aaacggcgtg gcgtgggaat ccatcgatgc ctggcagatc ccttcctcgc
    16141 tgattgccga aatgccggcc tccgtggcgc tgcattatgc ggtactgccg ctgcgtctgg
    16201 aaaatgatgt gttaattgtc ggcagtgaag atggtattga cccggtttcg ctggcggccc
    16261 tgacgcgtaa agtcggacgc aaagtgcgtt acgtcattgt tctgcgggga caaattgtca
    16321 cggggttacg tcactggtat gcacgccgac gcggtcacga tccgcgggca atgttgtaca
    16381 atgcggttca gcatcagtgg ctcacggaac agcaggccgg tgaaatctgg cggcaatatg
    16441 tgccgcatca gttcctgttc gccgaaatac tgaccacgct cggtcatatt aatcgttcag
    16501 caattaacgt gttgttattg cgccatgaac gcagttctct gccgctcggc aagtttttgg
    16561 tcaccgaagg cgttatcagc caggaaacgt tggatcgcgt cctgacaatt caacgcgaat
    16621 tacaagtttc gatgcaatca ctattactca aagcaggttt aaacacagaa caggttgcgc
    16681 aactggagtc cgaaaatgaa ggagaataac cttaatcgcg tcatcggatg gtctggttta
    16741 ctgctgacgt ctttattgag taccagcgca ctcgcagaca atatcggcac cagcgcagaa
    16801 gagctggggc tgagcgatta tcgccatttt gttatttatc cccgtctcga taaggcgctg
    16861 aaggcacaga aaaataacga cgaagcaacc gccatccgcg aatttgaata tatacaccag
    16921 caggtgccgg ataatattcc gctgacttta taccttgcgg aagcctatcg ccattttggt
    16981 catgatgacc gggcgcggtt gttgcttgag gatcaactga aacgtcaccc aggagatgcc
    17041 cgacttgagc gcagtctggc ggctattcca gttgaagtga aaagcgttac gacagttgaa
    17101 gaactgcttg cccagcaaaa agcgtgcgat gctgcgccga ccctgcgttg tcgcagtgaa
    17161 gtcgggcaga atgccctgcg gctggcacag ttacctgtcg ccagagcgca actgaacgat
    17221 gcgacgtttg ctgcatcgcc ggaaggaaaa acgctgcgaa ccgatctgct gcaacgggca
    17281 atctacctga aacaatggtc ccaggcagat acgctataca atgaagcacg ccagcagaac
    17341 acattaagcg cggcagaacg ccgtcagtgg tttgacgtgc ttcttgccgg gcagctggac
    17401 gatcggatcc tggcactgca atcacagggg atcttcaccg atcctcagtc atatattact
    17461 tacgcgaccg cgctgactta tcgtagcgaa aaagcacgcc tccagcgtta tctcattgaa
    17521 aataagccgc tgtttaccac ggacgcacaa gagaaaagtt ggctctatct gttatctaaa
    17581 tacagcgcca accccgttca ggcattggcg aattatacgg tgcagtttgc cgataaccgc
    17641 cagtatgttg ttggctcgac gctaccggtg ctgttaaaag aaggtcagta cgatgcagcg
    17701 caaaaactgc tcgccaccct ccccgccaat gaaatgcttg aggagcgtta tgctgtcagc
    17761 gtggcgaccc gtaacaaggc tgaagctctg cgtctggcac gcttgctgta tcagcaagaa
    17821 ccggcaaatc ttacccgcct ggatcaacta acctggcaac tgatgcagaa cgagcagtca
    17881 cgggaagctg ccgatttatt gctgcaacgc tatcctttcc agggcgatgc gcgtatcagc
    17941 cagactttaa tggcgcgact gacgtctctg ctggaaagtc atccttacct ggcaacgccg
    18001 gcgaaggtgg cgattttatc gaaaccctta ccgctggcgg agcaacgtca gtggcaaagt
    18061 cagttgccgg gtattgcaga taattgcccg gcaatagttc gcttgctggg cgatatgtcg
    18121 ccttcctacg atgccgccgc ctggaaccgt ctggcaaagt gttatcggga cacgctaccc
    18181 ggtgtggcgt tgtatgcatg gcttcaggcc gaacaacgac aaccgaacgc ctggcaacat
    18241 cgtgcggtag cctatcaggc gtatcaggtt gaggactaca ccaccgcact ggcggcctgg
    18301 cagaaaatca gtcttcacga catgagcaat gaggatctgc ttgctgctgc caataccgcc
    18361 caggcggcag gaaatggtgc ggctcgcgat cgctggctac aacaggcaga aaaacgtgga
    18421 ctgggaagca atgccctcta ctggtggctg catgcgcaac gttacattcc tggtcagccg
    18481 gaactcgcac tgaacgatct cacgcgctca atcaatattg cgccttctgc caacgcttac
    18541 gttgcgcggg cgacaattta tcgccaacgt cataatgtcc cggccgcggt gagtgatttg
    18601 cgcgccgcgc tggaactgga accgaataat agcaacaccc aggcagcgct tggttacgcc
    18661 ttgtgggata gcggtgatat cgcacagtcg cgggaaatgc tcgaacaggc gcataaaggg
    18721 cttccggacg atccggcact gatccgacaa ctggcctacg tgaaccagcg tctggatgac
    18781 atgcctgcga cgcagcacta cgcccggctg gtgattgatg acattgataa tcaggcgctg
    18841 ataaccccac tgaccccaga gcaaaatcaa caacgcttca atttccgccg tctgcatgag
    18901 gaggtcggtc gccgctggac gttcagtttc gattcttcca tcggcttgcg ttccggggca
    18961 atgagtaccg ctaacaataa tgttggcggc gcagcgccag ggaaaagcta tcgtagctac
    19021 gggcaactgg aagccgaata ccgcatcgga cgcaatatgc tgctggaagg cgacctgctc
    19081 tcagtttata gccgcgtctt tgccgatacc ggagaaaacg gggtgatgat gccggtgaaa
    19141 aatccgatgt ccggcaccgg tctgcgctgg aagccgctgc gcgatcagat ctttttcctc
    19201 gccgtcgaac agcagttgcc gctgaacggg caaaatggcg catccgatac catgctgcgc
    19261 gccagcgcct cattctttaa tggcggcaaa tacagcgacg aatggcaccc gaacggttca
    19321 ggctggtttg cccaaaacct gtacctcgat gcggcgcaat atatccgcca ggatattcag
    19381 gcgtggacgg cagattatcg cgtcagctgg catcagaagg tagctaacgg acagactatt
    19441 gagccttacg ctcacgttca ggacaacggc tatcgtgata aaggcactca gggcgcgcag
    19501 cttggcggtg tcggggtccg ctggaatatc tggaccggcg agacgcacta cgacgcctgg
    19561 ccgcacaaag tcagtctcgg cgtcgagtat caacatacct ttaaggcgat taatcaacgt
    19621 aacggagagc gcaacaacgc gtttctcacc attggagtgc actggtaaat gcgtaagttc
    19681 attttcgtat tgctgacact gcttttggtc agcccttttt cctttgcgat gaaaggtatt
    19741 atctggcaac cacaaaaccg tgatagtcag gttaccgata cccagtggca ggggctgatg
    19801 agtcagttac gtttgcaagg cttcgatacc ctggttttgc aatggacccg ttacggcgat
    19861 gcattcaccc agccagaaca gcgcgcgtta ttgtttaagc gtgccgcagc tgcgcaacag
    19921 gctggcctga agctgattgt cgggctgaac gccgatccgg aattttttat gcaccagaaa
    19981 cagtcatccg cggcgctgga aagctatctt aatcgcctgc tggctgccga tctccagcaa
    20041 gccagattat ggagcgccgt acctggcgta acgccggatg gctggtacat cagcgcggaa
    20101 attgacgacc tgaactggcg cagcgaagcc gcccgtcagc ctttgctgac atggttaaac
    20161 aacgcgcagc ggttgattag cgatgtttca gcaaaaccgg tttatatcag tagttttttc
    20221 gccggaaaca tgtcgccaga tggctatcgc caactgctgg aacacgttaa agcaaccggc
    20281 gttaatgtct gggtacagga tggcagcggc gtggataaac ttaccgctga acagcgtgaa
    20341 cgttatctac aggccagcgc cgattgccaa agctccgccc cagcaagcgg cattgtttat
    20401 gaactgtttg tcgctggcaa aggcaaaacc tttacagcga aaccgaaacc ggacgcagaa
    20461 attgcctcgc tgttagcgaa acgttcctct tgcggtaaag acactctcta tttctctctg
    20521 cgctatttgc ccgtcgcgca cggcattctc gagtattaaa tctcctccag gtaagtcggg
    20581 gacgacctgg cttacctctt tcgctctcgt cagaattcca tcaagattaa ttgctaaaaa
    20641 gcgtgctaaa gaaagaagat cgttatcggc gaacgggccg atcactcatg caggaagcat
    20701 agatcctttg agcggagttt ctgatatctg aggagtgcga aatgcaattg agcagcagtg
    20761 aaccttgcgt ggtgatcctg accgaaaaag aggtagaggt aagcgtcaat aaccatgcta
    20821 cgtttaccct tccgaaaaac tacctggccg ccttcgcgtg caacaataac gtcattgaac
    20881 tctcaacgtt aaatcacgta ttaatcaccc acatcaaccg taacatcatc aacgattatc
    20941 tgttgttttt aaataagaac ttaacctgtg taaagccctg gtcgcggctg gctaccccgg
    21001 ttatcgcttg tcatagccgt acaccggaag tgttccggct agccgccaac cacagcaagc
    21061 agcaacccag caaaccctgc gaagcggagt tgacgcgcgc attgcttttt accgtattgt
    21121 ctaactttct tgagcaatcg cggtttattg ccctactgat gtatatctta cgcagcagcg
    21181 tccgcgacag cgtctgccgc attattcaaa gcgatattca gcattactgg aatctgcgaa
    21241 ttgtcgccag ttcgctatgt ttaagcccca gcctgctcaa aaagaaatta aaaaacgaaa
    21301 ataccagcta tagccagatt gtcacagagt gtcgtatgcg ttacgccgta cagatgttat
    21361 tgatggataa caaaaatatc actcaggtgg cgcaattatg tggctatagc agcacgtcgt
    21421 actttatctc tgtttttaag gcgttttacg gcctgacacc gttgaattat ctcgccaaac
    21481 agcgacaaaa agtgatgtgg tgaagggcaa agcggaaacg gataagacgg gcataaatga
    21541 agaagaaatg gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg
    21601 ctctatccaa ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta
    21661 tacggtcaac ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc
    21721 gcgtctgatt ttctgattta tttcgctata gcggcaaaca aacgcacacc gctgcgcgtc
    21781 tgaatcaaga aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa
    21841 ttgtccgctc ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact
    21901 catcctatca tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt
    21961 gtcctgaaaa cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat
    22021 ttaatcatta tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc
    22081 aaacaaatcc agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat
    22141 gctggcagag cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat
    22201 gatatttttc atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg
    22261 ctcaactata taaaaagcaa taaatgtagt acgaatagtt caacgatcac tgtactatct
    22321 aatcgtgaag tgacgatatt acgttatct
//
LOCUS       DAPGRN010000080        22155 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPGRN010000080 DAPGRN010000000
VERSION     DAPGRN010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.080"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(272..1192)
                     /gene="malM"
                     /locus_tag="RHW33_003163"
     CDS             complement(272..1192)
                     /gene="malM"
                     /locus_tag="RHW33_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV1646641.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLAEVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPVPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1344..2684)
                     /gene="lamB"
                     /locus_tag="RHW33_003164"
     CDS             complement(1344..2684)
                     /gene="lamB"
                     /locus_tag="RHW33_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV1646642.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNDIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNANTNTNFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2756..3871)
                     /gene="malK"
                     /locus_tag="RHW33_003165"
     CDS             complement(2756..3871)
                     /gene="malK"
                     /locus_tag="RHW33_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV1646643.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4236..5426
                     /gene="malE"
                     /locus_tag="RHW33_003166"
     CDS             4236..5426
                     /gene="malE"
                     /locus_tag="RHW33_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV1646644.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5580..7124
                     /gene="malF"
                     /locus_tag="RHW33_003167"
     CDS             5580..7124
                     /gene="malF"
                     /locus_tag="RHW33_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV1646645.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7139..8029
                     /gene="malG"
                     /locus_tag="RHW33_003168"
     CDS             7139..8029
                     /gene="malG"
                     /locus_tag="RHW33_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV1646646.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8401..9876
                     /gene="xylE"
                     /locus_tag="RHW33_003169"
     CDS             8401..9876
                     /gene="xylE"
                     /locus_tag="RHW33_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV1646647.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(9920..10330)
                     /gene="psiE"
                     /locus_tag="RHW33_003170"
     CDS             complement(9920..10330)
                     /gene="psiE"
                     /locus_tag="RHW33_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV1646648.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10456..10599)
                     /locus_tag="RHW33_003171"
     CDS             complement(10456..10599)
                     /locus_tag="RHW33_003171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646649.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10600..10878
                     /locus_tag="RHW33_003172"
     CDS             10600..10878
                     /locus_tag="RHW33_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646650.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10925..13021)
                     /locus_tag="RHW33_003173"
     CDS             complement(10925..13021)
                     /locus_tag="RHW33_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV1646651.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPTYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13021..13758)
                     /locus_tag="RHW33_003174"
     CDS             complement(13021..13758)
                     /locus_tag="RHW33_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV1646652.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERANPPLQGNYTLWVGPPPSTVTLFGLISRPGNQP
                     FTPGRDVASYLSGQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSEMPDALNADILQTLTQRIPQ"
     gene            complement(13755..14393)
                     /locus_tag="RHW33_003175"
     CDS             complement(13755..14393)
                     /locus_tag="RHW33_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV1646653.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     AVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14507..14749)
                     /locus_tag="RHW33_003176"
     CDS             complement(14507..14749)
                     /locus_tag="RHW33_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646654.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15261..16910)
                     /gene="pgi"
                     /locus_tag="RHW33_003177"
     CDS             complement(15261..16910)
                     /gene="pgi"
                     /locus_tag="RHW33_003177"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV1646655.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17435..18784
                     /gene="lysC"
                     /locus_tag="RHW33_003178"
     CDS             17435..18784
                     /gene="lysC"
                     /locus_tag="RHW33_003178"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV1646656.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            18929..19858
                     /locus_tag="RHW33_003179"
     CDS             18929..19858
                     /locus_tag="RHW33_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDV1646657.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIVDSCFSSQVQDSDDELSQLV
                     SYRDNLYSTPAKLVDFTQEKLRLQQVRKKYQREFNNTAHSENKASFDSIWQRLTNHDV
                     TSQQSPIGFVLGGQPGAGKSALIELAKRETKNNIMIINGDDFRFLHPDFNYIYQTYGD
                     DFVTHTAKFSGETVERAIERAIANKLNIVVEGTFRNAATPLQTLKKLKDAGYRTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTDLLAENASKVFASNLADKFA
                     VYSREKMIFSSQAATNCDIATLIQNEISGSAQE"
     gene            complement(19891..20682)
                     /locus_tag="RHW33_003180"
     CDS             complement(19891..20682)
                     /locus_tag="RHW33_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDV1646658.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYGVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPTWFSPLRARWHYYSMAQKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFSELLAGSELDAALRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIHAELARGEIPPVLPDSREGDVKKLDSLLYQTVMRRA"
     gene            complement(20688..21815)
                     /locus_tag="RHW33_003181"
     CDS             complement(20688..21815)
                     /locus_tag="RHW33_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDV1646659.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILLEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVPVQTEVMNAVLQPRRKGVVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREVVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         5297 a         5792 c         5553 g         5513 t
ORIGIN      
        1 tcaaagagtt attgccgtca aactttgaga tatgacctaa cataaaacct ctgcattaca
       61 gaaatgaaat aactggaaaa agtagcctga gcgtccagac actaccggcg aaaggaaaag
      121 aatctctgat aatgcattga gataatggat attcttagtg aagaagaagt gaagtactat
      181 aaaaaatcgc tcatatccct ggaatgaagc ataaaaaaag gtgcgccagg agacgcacca
      241 gttgttgcaa aatcagcact gtggggcgta attacccctt gccttttaca ctgctgataa
      301 aggtggaacg ggcagatgtc gatcccaggc gttcagcttc atcaagcagt tttaacgcct
      361 tatcaacatc acctttcgcg acagcgtttt tgatcgcggt attaaaataa ctttccgtgt
      421 cgttgagcat cggctcgctt ttcttcaccg gtgccggaac gggtgcagcc acagctggtg
      481 ccgccgtgtt gcctaccgta accggagctg gagcggaaga accaaataaa ggcccgacca
      541 acacgctgga gctggagttc gttttcactt tcagtttcag taagccatcg ctggtatgac
      601 gagcaaccgg atcgggaata tccgggatcg agttaccgac gcctttggca taggctttag
      661 ccgggtcgag cagttgggtc gtctgctgga gatctttttc cgtggtaaag accagaacat
      721 aaagtttttg ctgccccaac gccggtgtca ggcgcataac gccttccaga cgatctgcac
      781 tcatcacgcc tggttcctgg taggtgaaat aactgctggg gaagaaggct gatggggtca
      841 tgttctgatc aagaatcagc acgttcggcg caaatacgct agtttgtttg ttcacttcgc
      901 tggtcagcgt cagggtcagt tcgccaatgt ttgccgggac gctgtacgcg gcaaccggac
      961 cactgatgcc gggaacgttc agttgttggc cgccagtcgc cagttgggtg gtctgggttt
     1021 tagattgatc gaccggtgtc caggtgagtt gttgcagcgc ggcagatgga atggctggcg
     1081 cgtcgctggt gttttgcggt acgtagttaa cttcggcaag gctgattcca ggcgcgcttg
     1141 ccagcaaccc tgctgataaa cagagggcga tgagactttt attcattttc attgttttca
     1201 cctcaaaatc tggagctcag cggtagccag gcaatagcgc gctaaacccg ataatcagag
     1261 gggcttgcgc ccctttcaga taatgacaac ctgtttttat gccggatgcg gcgcaagcac
     1321 cttatccggc ccaggttttg ctattaccac cagatttcca tctgggcacc gaaggtccac
     1381 tcgtcgctgt cgccacgacc gaagctgccg ccgttgaaat cagcaggaac ggctttgccg
     1441 aagttagtgt tggtattggc gttaccggtg tagtcgtaac cccatttctc atcccacttg
     1501 gcgtaggttg cgaagacacg aatagccggg cgtgaccaga tgctgtcgcc agcctgccat
     1561 tgttgtgcga gggtaatttt gtactgattg ttcttgtcgc cggtgcgctg ggattcgacg
     1621 ttgtcgtagc cgatttccat cacggtgctc atgattggcg tccacttgta catcgggcga
     1681 ataccgacgg tccaccactt ggtgccgttg tcgttatccc agttgatatc ctggtacata
     1741 cccacgtaca tcatgtccca gttgtcgccc atggagatcg caccgtggtc gaggatacgc
     1801 agcatgtgac cgttgttgtt gatattgtag gcaaattttt cgttatcaaa tgcaacgcca
     1861 gaaccctgcg acagaccttt accctgcgag gtcatcgagt cagtagcgta ctgaacaaca
     1921 aacttgttaa agcccttcag gacactctga gtatgttcag cagtgaataa ccagccgtct
     1981 ttcgatgcgc catcaaccag acgatagtta tcacgcaggt tggcacgacc gtagtcgaca
     2041 cccagttcta acgtgccgcc cgggttgatt tccatctgcg ctaaacgcac atcgaaaacg
     2101 tcgttcgcgg tttcgttggt atagtcataa atatcgttgc tggcgaaaga ggaagaacca
     2161 cctgcttcag aggagcgggt tgctgccaga gagagtttac cgaagccaac atcgatgttt
     2221 tccagaccgg caccaggacc agaaatatcc cagtagtaga agtcgatcat atgaacgtca
     2281 tgacgttggt agaagcgctt acctgcccag atggtggagc ctggcagcca ttcgatcagg
     2341 ttcttaccct gcacgtttgc ttcacggaag gccggatcgg tagcttccca gtcattctgt
     2401 tgcgcgacgg aataggccac gttagtgtcg aaatagaagc tcttatcgcc ctctttccac
     2461 acttcctgac ccaattttaa ttcagcataa gtttcacatt cgttgccaag acggtattta
     2521 ctttgagcac cggtagtctg gaaacactgt tgttcaccgc cgctacctgt ccagccaata
     2581 ccggaacgtg catagccgtg gaaatcaaca gccattgcct gagcagacat tacgcccgct
     2641 gcgacggcaa ccgccagagg aagtttgcgc agagtaatca tcattctatc tcctgagtca
     2701 ttgcttttct tttttcacat cacctgtgac aggctttgtg tgttttgtgg ggtgcttaaa
     2761 cgcccggctc cttatgcagt cgacgacatg cagtgccatc ctcacggaac agatggcaac
     2821 gctctggcgg caggccgata gcgaatgtgg caccttcttc taccaacacc acgtcgttct
     2881 ggcggtacac caggttttga cgaatggaag ggatctggat atggatttgg gtttcgttgc
     2941 cgagttgctc gacgacctga acttcaccct caaggatgac gtcagcgata tcactcggca
     3001 gtagatgttc cgggcgaata cccagcgaca tattggctcc aacctggaca tcacggcttt
     3061 caacaggtag ccagacttgc tgacgatttg gcatcggcag ctccacctgc acttgatcga
     3121 ttgcagtggc ggtcactttt accggcagga agttcatctt tggcgaaccg ataaatccgg
     3181 cgacaaaacg gtctgccgga tagtggtaca gctccagcgg tttcccaacc tgcgccacgc
     3241 gaccggcgtc cagcaccacg attttgtcgg ccagcgtcat cgcttcgacc tgatcgtggg
     3301 tgacgtaaat cattgtgcgg cccaggcgtt tatgcagacg ggagatttcg atacgcattt
     3361 gcacacgcag tgcagcatcg aggttggaga gcggttcatc gagcaaaaat acgcttggct
     3421 cggccaccag cgtacggcca atcgccacgc gctgacgctg accaccggag agcgctttcg
     3481 gtttgcgatc cagcaaatgc gccagttgta gcacttccgc cacctggttg acgcgttggt
     3541 taatcacctc tttttttgcg cctgccagtt tcaggccaaa tgacatgttt tctgctactg
     3601 acaggtgggg atagagcgcg taagactgaa acaccatacc aacgccgcgt tctgctggcg
     3661 gagtgtcatt catccgtttc tcaccgatga acaggtcgcc gctggtgatt gtctcaagcc
     3721 cggcaatcat gcgcagtaaa gtcgatttac cgcagccaga cggtccgaca aacaccacga
     3781 attcaccttc atggatatcg agattgatat ctttcgatac cacgacctcg ccccaggctt
     3841 tcgttacatt ttgcagctgt acgctcgcca tgcccttctc cctttgtaac aacctgtcat
     3901 cgacagcaac attcataatg ggctgactat gcgtcatcag gagatggctt aaatcctcca
     3961 ccccctggct tttttatggg ggaggaggcg ggaggatgag aacgcggctt ctgtgaacta
     4021 aaccgaggtc atgtaaggaa tttcgtgatg ttgcttgcaa aaatcgtggc gattttatgt
     4081 gcgcatctcc acattaccgc caattctgta acagagatca cacaaagcaa cggtggggcg
     4141 taggggcaag gaggatggaa agaggttgcc gtataaagaa actagagtcc gtttaggtgt
     4201 tttcacgagc acttcaccaa caaggaccat agattatgaa aataaaaaca ggtgcacgca
     4261 tcctcgcatt atccgcatta acgacgatga tgttttccgc ctcggctctc gccaaaatcg
     4321 aagaaggtaa actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag
     4381 tcggtaagaa attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac
     4441 tggaagagaa attccctcag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg
     4501 cacacgaccg ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca
     4561 aagcgttcca ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc
     4621 tgattgctta cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc
     4681 cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag
     4741 gtaagagcgc gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg
     4801 ctgacggggg ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg
     4861 tggataacgc tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac
     4921 acatgaatgc agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag
     4981 cgatgactat caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg
     5041 gtgtaacggt actgccgacc ttcaagggtc aaccatctaa accgttcgtt ggcgtgctga
     5101 gcgccggtat taacgccgcc agtccgaaca aagagctggc gaaagagttc ctcgaaaact
     5161 atctgctgac tgacgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag
     5221 cgctgaagtc ttacgaggaa gagttggcga aagatccacg tattgcagcc accatggaaa
     5281 acgcccagaa aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg
     5341 tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag
     5401 acgcgcagac tcgtatcacc aagtaatgct gtgaaatgcc ggatgcggcg tgaacgcctt
     5461 gtccggccta caaaaccgaa acgtatgtag gtctgataag acgcgtcagc gtcgcatcag
     5521 gcagttgttg tcggataagg cgtgaaagcc ttatccgtcc tggaatgagg aagaacccca
     5581 tggatgtcat taaaaagaaa cattggtggc aaagcgacgc gctgaaatgg tcagtgttag
     5641 gtctgctcgg cctgctggtg ggttaccttg ttgttttaat gtacgcacaa ggggaatacc
     5701 tgttcgccat taccacgctg atattgagtt cagcggggct gtatattttc gccaatcgta
     5761 aagcctacgc ctggcgctat gtttacccgg gaatggctgg aatgggatta ttcgtcctct
     5821 tccctctggt ctgcaccatc gccattgcct tcaccaacta cagcagcact aaccagctga
     5881 cttttgaacg tgcgcaggaa gtgttgttag atcgctcctg gcaagcaggc aaaacctata
     5941 attttggtct ttacccggcg ggcgatgagt ggcaactggc cctcagcgac ggcgaaaccg
     6001 gcaaaaatta cctttccgac gcttttaaat ttggcggcga gcaaaaactg caactgaaag
     6061 aaacgaccgc ccagcccgaa ggcgaacgcg cgaatctacg cgttattacc cagaatcgtc
     6121 aggcgctgag tgacattacc gccattctgc cggatggcaa caaagtgatg atgagctccc
     6181 tacgccagtt ttctggcacg cagccgctct acacactcga cggtgacggc acgttgacga
     6241 ataatcagag cggcgtgaaa tatcgtccga ataaccaaat tggcttttac cagtccatta
     6301 ccgccgacgg caactggggc gatgaaaagc taagccccgg ttacaccgtg actactggct
     6361 ggaaaaactt tacccgcgtc tttaccgacg aaggcattca gaaaccgttc ctcgccattt
     6421 tcgtctggac cgtggtgttt tcactgatca ccgtcttttt aacggtggcg gtcggcatgg
     6481 ttctggcatg tctggtgcag tgggaagcgt tgcgcggcaa agcggtctat cgcgtcctgc
     6541 tgattctgcc ctacgcggtg ccatcgttca tttcaatctt gattttcaaa gggttgttta
     6601 accagagctt cggtgagatc aacatgatgt tgagcgcgct gtttggcgtg aagcccgcct
     6661 ggttcagcga tccgaccacc gcccgaacga tgttgattat cgtcaatacc tggctgggct
     6721 atccgtacat gatgatcctc tgcatgggct tgctgaaagc gattccggac gatttgtatg
     6781 aagcctcagc aatggatggt gcaggtccgt tccagaactt ctttaagatt acgctgccgc
     6841 tgctgattaa accgctgacg ccgctgatga tcgccagctt cgcctttaac tttaacaact
     6901 tcgtgctgat tcaactgtta accaacggcg gcccggatcg tcttggcacg accacgccag
     6961 ccggttatac cgacctgctt gttaactaca cctaccgcat cgcttttgaa ggcggcgggg
     7021 gtcaggactt cggtctggca gcagcaattg ccacgctgat cttcctgctg gtgggtgcgc
     7081 tggcgatagt gaacctgaaa gccacgcgaa tgaagtttga ttaagggaga taacaaaaat
     7141 ggcaatggtc caaccgaaat cgcaaaaagc acgtttgttt attactcacc tgctactgct
     7201 actttttatc gcggcgatta tgttcccgct gctgatggtc gtcgctatct cgctgcgtca
     7261 gggaaacttc gcgaccggta gcctgatccc ggagcaaatc tcctgggatc actggaaact
     7321 ggcgttaggt tttagcgttg aacaggctga tggtcgcatt acgccaccgc cattcccggt
     7381 actgctgtgg ctgtggaact cggtaaaggt cgccgggatt tccgcgattg gcattgtggc
     7441 gctctccacc acctgcgcct acgctttcgc ccgtatgcgc tttccaggca aagcgacgct
     7501 gctgaaagga atgctgattt tccagatgtt cccggcagta ctttcactgg tcgcgttgta
     7561 tgcgttgttt gatcgtctgg gtgagtacat tccattcatt ggcctgaata ctcacggcgg
     7621 cgtaattttc gcgtatctgg gtgggattgc gctgcatgtc tggaccatca aaggctattt
     7681 cgaaaccatc gacagttcgc tggaagaagc ggcggcgctg gatggtgcga caccgtggca
     7741 ggccttccgc cttgtcctgt tgccgctgtc agtaccgatt ctggcggtgg tgttcatcct
     7801 gtcgtttatc gctgccatta ctgaagttcc ggtcgcatcg ctgttactgc gtgacgtaaa
     7861 cagctacacc ctggccgtgg ggatgcagca atacctcaac ccgcaaaact acctgtgggg
     7921 tgactttgcc gccgctgcag tgatgtctgc attaccgatc accatcgtct tcttgctggc
     7981 tcaacgctgg ctagtcaacg gcctgacggc aggtggtgtg aaaggttaaa gatgttgttc
     8041 tgccaatgtt atgccgctgc accctcaact tacgttatcc caacttgtga ctgttattcg
     8101 gcgctccacg gagcgccttt ttttctttcg tctgcaatct gaattgttcg ccggttaata
     8161 tttccatcat agagcttatt atttttacgt tatttgtttt cccacttacg ataattctct
     8221 ttcgtgctct gagtcacggc aatagtattg ttttcatcaa ttttagataa ttatcacaat
     8281 taagatcaca gaaaagacat tacgtaaacg cattgtaaaa aatgataatt gccttaactg
     8341 cctgacaatt ccaacatcaa tgcactgata aaagatcaga atggtctaag gcaggtctga
     8401 atgaataccc agtataattc cagttatata ttttcgatta ccttagtcgc tacattaggt
     8461 ggtttattat ttggctacga caccgccgtt atttccggta ctgttgagtc actcaatacc
     8521 gtctttgttg ctccacaaaa cttaagtgaa tccgctgcca actccctgtt agggttttgc
     8581 gtggccagcg ctctgattgg ttgcatcatc ggcggtgccc tcggtggtta ttgcagtagc
     8641 cgcttcggtc gtcgtgattc acttaagatt gctgctgtcc tgttttttat ttctggtgta
     8701 ggttctgcct ggccagaact tggttttacc tctataaacc cggacaacac agtgcctgtt
     8761 tatctggcag gttatgtccc ggaatttgtt atttatcgca ttattggcgg tattggcgtt
     8821 ggtttagcct caatgctttc accaatgtat attgcggaac tggctccagc tcatattcgc
     8881 gggaaactgg tttcatttaa ccagtttgcg attattttcg ggcaactttt agtttactgc
     8941 gtaaactatt ttattgcccg ttccggtgat gccagctggc tgaatactga cggctggcgt
     9001 tatatgtttg cctcggaatg tatccctgca ctgctgttct taatgctgct gtataccgtg
     9061 ccagaaagtc ctcgctggct gatgtcacgc ggcaagcaag aacaggcgga aggtatcctg
     9121 cgcaaaatta tgggcaacac gcttgcaact caggcagtac aggaaattaa acactctctg
     9181 gatcatggcc gcaaaaccgg tggtcgtctg ctgatgtttg gcgtaggcgt gattgtcatc
     9241 ggcgttatgc tctccatctt ccagcaattt gtcggcatca atgtggtgtt gtactacgcg
     9301 ccggaagtgt tcaaaacgct gggggccagc acggatatcg cgctgttgca gaccattatt
     9361 gtcggagtta tcaacctcac cttcaccgtt ctggcaatta tgacggtcga taaatttggt
     9421 cgtaagccac tgcaaattat cggcgcactc ggaatggcaa tcggtatgtt tagcctcggt
     9481 accgcgtttt acactcaggc accgggtatt gtggcgctac tgtcgatgct gttctatgtt
     9541 gccgcctttg ccatgtcctg gggtccggta tgctgggttc tgctgtcgga aatcttcccg
     9601 aatgccattc gtggtaaagc gctggcaatc gcggtggcgg cccagtggct ggcgaactac
     9661 ttcgtctcct ggaccttccc gatgatggac aaaaactcct ggctggtggc ccatttccac
     9721 aacggtttct cctactggat ttacgggtgt atgggcgttc tggcagcact atttatgtgg
     9781 aaatttgtcc cggaaaccaa aggtaaaacc cttgaggagc tggaagccct ctgggaaccg
     9841 gaaacgaaga aaacacaaca aactgctacg ctgtaatctt cctgtccagc acgccgcgcc
     9901 agttcggcgt gctgtctttt tactcccgct tcagccgttt cgaattacac agccacaggg
     9961 tgatcaccag taacaggatc gcagccgagt agatcaacac atccagtggc gatttatgat
    10021 cgacgatgat caagcgcaca atggcggtga tcccaatata gacaaagtaa cgtagcggga
    10081 agtgaaaacc ggactgaaag tacttcacaa tcagcgcgat aaattcgaaa tagagaaagt
    10141 aaaccaccag tccttctacc agctcatatt tgctggtttg ttctggcgcg aacagcacat
    10201 cagccagatg caccgtttct ttgccgagga agacaaccaa aataaggccg aggcacagca
    10261 ggccaagatt gagtacggtc tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga
    10321 gtgacgtcat agcagtacct ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg
    10381 acggagatct atattttttg tgtatgtttt gttcaacctg gatttggagc gcgcctcctc
    10441 caaagccgga tatacctacc cgtaggccgg ataagccgcg cctggcggcg catccggcaa
    10501 gacaaaccgc acccaagccg gtaattcaaa aatcacccat tcaggtgatt cccagccacc
    10561 agcaatgcgc gaaaaatcga caatgttcct ctggaacaaa tgaaacgtaa cctgataaag
    10621 gtggtaaaaa tgaagcctta ttttgctgct ttgatgttat cagtctctgt tcttcctgct
    10681 tatgctggtc cgctcggtac tgctgataaa gccgacctac cgcaaagcaa tgtctccagc
    10741 ccaatgatgg cccagtcgct caggcaacca gacctgcaac caatatcgac ggacaggaag
    10801 acagaatgtt ttcgccttta cacaccagat cgcaaaccgg gggtgaactg cgttcctgat
    10861 ggctcgacag gccattaaga atgaaggtgc cagtgagtgc gaaaactggc acctttccca
    10921 tgacttagcg gaaattgacg ctacggtcgc tggtcatgtc atacaactgg aacttacgcc
    10981 caagttgctg accaccgtca cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg
    11041 tcgggccaga cgagaagaga tccaacggta ccgagacata cacgcctttg gtgaagtccc
    11101 cttcgccgta ctcctctttc gagacgttgg tgatcgtggc atagccgccc accaccacgc
    11161 cgctgtcaaa gcgtttggcg atctccagcg tgccgccttt atcccctgcc agatactgcc
    11221 caacgctcgc tttcaccaac acgtcctggg cgaaagatgg cgtccagtac gcggtcagat
    11281 gtccggtttt cacgctgtaa tcggtgaatt tcatcatatc tttcgcgcta cgccagtcgc
    11341 gctgtttaac gtagttggca tccagaccaa acgcccagtt gctgtccagc gggcgataca
    11401 acacctcagc ccccgcaccg ccaaacatgg tttcgagata gccgccgtag acctgaccgt
    11461 agaagccgtt gcccagatgc tggaagtagt tggcttgcag gttattcaca tagacatcgt
    11521 tctgcacata ctcgcgcaca tgggtacgca cgcgcggtaa gtgcgagtcc tgcggcggat
    11581 tggtgtagtt aaacttgtcg tagttgttgg caagatttgc aaacaggctg ccggtggtca
    11641 gcagatggtc cgtcagccac aaatctgccg ttcccatcac gcccagctga tacatgtaaa
    11701 agttttccgg gccaccgacc gactggttca gcaccggatc gatatggaaa tcaaagcgcg
    11761 atttgtcgat ataccatccc tgctcggtgg acttcggaac cactggctcg acgcgttttt
    11821 gcgccagctt cgtttcgtgg cccaacggct ctccggcgag atgatttttc aggctggcga
    11881 catcggtttc cgtcgtcacc tgcggcatgt taaggcgatt ttccgtgaca cggatcgttt
    11941 tgatcccatc cggcagatcg ttcatcacga tccgattagc acggatgatc ccttcgcgcg
    12001 aatcacgata tttcacctgc tcgccggtaa catacagcgt atcgcctttc gcctggatct
    12061 gcggatcggc aagtccggca ttgtatttca acagcgttaa ctgattcgcc accaccgagt
    12121 gctgcaaaat ggcatcctgt ggctgcggtt ggtattgcgg gcgggcgtta tcgttgtaag
    12181 tcgggcgcag atcgttaaag ttggtgcgca acgtaacgcc aaacataaag gtgttgccac
    12241 gttcatagct aaggttaacg tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa
    12301 acttactttt ttgctccagc ttcccggcaa aatcctgctg ataattattg ccttcatact
    12361 ccagtttcag gcgcagcggt tgccagggcg tctggtattc cacgccgcca aacagtgagg
    12421 caggaccgtg gaacatctgg ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc
    12481 gatagcaata tttatcactg gctgaacaga gcggattttt cacattaccg ctggtgccca
    12541 gatatcccca gcccaggccg agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa
    12601 gatattccgc atcaaacagc cccgtaccgc cgatatcccg cgcaccaacc gccacttgcg
    12661 gcagccagta actctcttcc cacaaacgca gtttgagatc gaaggcttta tctttatacg
    12721 tttgatcgcc agagaacgct tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc
    12781 gcagcgttgt ttccagccac gggaagagtt gcactgaagc tgagtaataa cggtactgat
    12841 cgttatcgcg atagttcaga ctcaactccc cttcccgcgc catgcgagcc gtgggcgttt
    12901 gtaataatcc tacgccaccg aaatccgact gcgacggacc aatgggcgca ggatatgttt
    12961 cgccgtagca agctgtgcta atgcccagcg ccagtaagct aagcagatgt ctttttttca
    13021 ttattgaggt atccgctgcg tcagagtctg aagaatgtcg gcgttaaggg catcaggcat
    13081 ctcactccag acggagtccg cgaggccaac ataaataatg ctgccgggca tcggctctac
    13141 gtgacgcttg ttccagtaag ccaccggcgc tttttgtgtg cgtccgtccg ggtaaaccac
    13201 ccacgcgtag ctacgatccg caccgctaag cagactttgc ccggagagat agctcgccac
    13261 gtcgcgaccg ggagtgaatg gctgattgcc aggacggctg ataagcccga acaacgtgac
    13321 cgtggacggt ggtggtccga cccacagcgt gtagttgccc tgcaacggcg ggttagcgcg
    13381 ttcagcaacg cggacgatat cgggatcaag attgattttt tgcctgcccg tcaccttcaa
    13441 cgcctgaatt tgctggcgta aggcgttaat cgcagcggca tcgtcggcgc tggaatctgc
    13501 gccctgttct gccagccgtg tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc
    13561 cagctcttcg ctgatcaccg caccgggcca ccagctgttt gccagccgcg gttggcctac
    13621 cagatcgatt aaatgctctg cgcctgttag tgttttggcc tcactgctgc cattgctgaa
    13681 caccttgacg gttcctgccg cgaagaccga tgacgctccc acactcaaaa gcaacgcgac
    13741 aaaagtttgt ttgatcattg ttttgccgcc ttgatgaggg tggttttcac cgggaaataa
    13801 tccgcgccga gatactgttc cgactggcga atttgccctt cgctgtcgat ccaatagcgg
    13861 ttatgccagc gcgcctggtc ggtggagact tcttcgtcca gcacgcgaac cgcggtttca
    13921 tcgctgccga ctttgacggt atcggtgcca tcccatttga agactgagcg tgcggtggcg
    13981 tagcgtacct gctggtattc ggtccagccc atcgtgcgcg tccaggttgc accatcaatg
    14041 atttgtgcgg gtttaatcag cgggtcggcg gcaaggttat tcacctcaat caagttgtcg
    14101 ccgccaagca gagtcttcac cagacgacca tgttgtgtga cgagagtagc ctgatcctga
    14161 gtgacccatt tttgttgtcc gtcttcagcg aaggccagca ccacaaacaa ctgcggcccg
    14221 ccattaagct gcatgtactg gctggcgtag ggcatatttt gaatatcatc gtccgtcagc
    14281 tgtacacctg gcgtaccgaa cagactgtcc cacagtgaat tgcccagctc tttagtcgtg
    14341 gctgaacagg cctgtaatag caggcagata agaatgagtg caggtcgctt cacgactctt
    14401 ctccagaggg gcagaataac cacaccggag tgtggttatg gtaaatccct tcatacttca
    14461 agccacagat gcgatgactc gaattatttt ggggatacat actttattac tgggtactgg
    14521 tggtcgtggt tgttgtggtc ccggtattag aaccatcacc gccaccggtt gccgcgagcg
    14581 cgacacccac cgccgagctt acggtgctga cgctggtcgc ggaggaacta cccgccgaaa
    14641 ccgacgttgc tgcagaccct gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag
    14701 tcgccgcaag cgcagatatg gcaaaaatgc catacagaac ttttttcata acaatttccc
    14761 ttcattgaat gaatggagat ttacccaaaa acatttcggg cgggatcgag tatacacaac
    14821 taaagcatgc gaattacggc gcggggaatt agcgatggcg ttttaggata attggaatga
    14881 atatcaaata taagtttcaa cttatcttta aataacaata aaaatcataa tgttaaaata
    14941 aaagtaaaag taattaatct gagtattttc cgaaatcaat cacgattaaa aatctatttt
    15001 aggattaacc tcacggtatg atttccgtta aatcacagac aaggggattt atctgataaa
    15061 acaatgccga cataacgtcg gcattgttat taaggttatt taatcaatcg tgctatgtat
    15121 taattgccga atacgatgta catatcggca tcgacctgta ggcctgataa gacgcgaccg
    15181 cgtcgcatca ggcatcggtt gccggatgcg gcgtgaacgc cttatccggc ctacatagcg
    15241 gcatcaggca tgaacgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg
    15301 gttgagctat cgtggctgct gatttcttta tcatctttca gctctggcag aatacggttc
    15361 gccagttgtt tacccagctc cacgccccac tggtcgaagg tgaagatgtt caggatcacg
    15421 ccctgagtaa agattttgtg ctcatacagc gcaatcaacg cacccaggct gaacggagtg
    15481 atttcgcgca gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc
    15541 acgtagtcaa gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg
    15601 cgggatttac caaacgccag cgcttcggtc tgagcaaaga agttagacag cagtttctgg
    15661 tggtgatcag agagcgggtt atgggtgata gctggggcga tgaaatcgca cggtaccatt
    15721 ttggttccct ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc
    15781 cagataatcg ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg
    15841 ttggactcca tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac
    15901 ggcagaatcg cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc
    15961 aacagtacag gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt
    16021 gcgccagaaa gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca
    16081 atcgctgacc acaaagagta acggccgcca acccagtccc agaactcgaa catgttggcg
    16141 gtatcaatac caaactcgcc aacggctttg gcattggttg agagcgccgc aaagtgtttt
    16201 gcaacgtgtt tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg
    16261 gtcatggttt cctgagtggt gaaggtttta gatgctacca ggaacagcgt ggtttccggg
    16321 tttacttttt tcagcacttc cgcaatgtga gtcccatcga cgttagaaac aaagtgcatg
    16381 ttcaggtgat ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa
    16441 ccgccgatcc cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca
    16501 ccggagataa tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc
    16561 attacgtctt tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc
    16621 agcacggcgc ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg
    16681 cccgccagat cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg
    16741 cggtttttgg agtaatccac cagcatctga tcgtcgaagg ttgcagagaa cttagaaaaa
    16801 cgatcgccgt ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt
    16861 ttctgtagtg cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact
    16921 cttctgattt tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga
    16981 tggttttagt gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat
    17041 tagctgaact actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa
    17101 ccgccccgct caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg
    17161 cgcgttgccc aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc
    17221 atcgccgagg tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc
    17281 tgggttgtca ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg
    17341 tgaaaatagt agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca
    17401 aggctgaaaa tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt
    17461 tggcggtacc agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc
    17521 tgatgccaac gtgcgtttag ttgtcctctc ggcttctgct ggtatcacta atctgctggt
    17581 cgctttagct gaaggactgg aacctggcga gcgattcgaa aaactcgacg ctatccgcaa
    17641 catccagttt gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga
    17701 acgtctgctg gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc
    17761 gctgacagat gagctggtca gccacggcga gctgatgtcg accctgctgt ttgttgagat
    17821 cctgcgcgaa cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa
    17881 cgaccgattt ggtcgtgcag agccagatat agccgcgctg gcggaactgg ccgcgctgca
    17941 gctgctccca cgtctcaatg aaggcttagt gatcacccag ggatttatcg gtagcgaaaa
    18001 taaaggtcgt acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc
    18061 ggaggcttta cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac
    18121 cgatccacgc gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc
    18181 agagatggca acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg
    18241 cagcgatatc ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt
    18301 gtgcaataaa actgaaaatc cgccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac
    18361 tctgctcact ttgcacagcc tgaatatgct gcattctcgc ggtttcctcg cggaagtttt
    18421 cggcatcctc gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt
    18481 ggcattaacc cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc
    18541 tctgctgatg gagctttccg cactgtgccg ggtggaggtg gaagaaggtc tggcgctggt
    18601 cgcgttgatt ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt
    18661 actggaaccg ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt
    18721 cctggtaccc ggcgaagatg ccgagcaggt ggtgcagaag ctgcatttta atttgtttga
    18781 gtaatatttc cctactggcg tataacgctc accaattgtt atacgccatc ctcactgaaa
    18841 acatagatta aataatatct gaaaagcaaa atcactcctg tagaacagta ttacaatcac
    18901 cgaaaaatat catttaacag gagtgttaat ggctacttta atggaaaagg atgcactttt
    18961 aaacggcgcc agtcagtgca tcgcgttttt atcaaatatc gtcgacagtt gtttttcctc
    19021 acaagttcag gactcagacg atgagctatc acaattagtc agctatcgcg acaatcttta
    19081 ttccactcca gcaaaacttg ttgattttac acaggaaaag ttacgcctcc agcaggtcag
    19141 aaaaaagtat cagcgtgaat ttaataatac tgcgcattca gaaaataaag cgtcttttga
    19201 ttctatttgg cagcgtctga caaaccatga cgtcacatcc cagcagagtc ccattggttt
    19261 tgttttaggt ggacaaccag gagccggaaa atccgcccta atcgaattag caaaaaggga
    19321 aacaaagaat aatataatga ttattaatgg tgatgatttc agatttctac accctgattt
    19381 taattatata tatcaaactt atggcgacga ttttgttacc catacggcaa aattttccgg
    19441 cgaaacggtc gaacgtgcaa tagagagggc catagccaat aaactcaata ttgttgtcga
    19501 aggaacattt cgtaatgccg ctacgccgct acaaacatta aaaaaactca aagatgcagg
    19561 ctaccgaaca gaagtcatga taaaaaccac ctcagcggca ttaagctggg aaagcaccaa
    19621 cgaacggtat aataaggaca aagaggcggg aaatatcgcc cgcaaagtgg ataaaaatca
    19681 ccatgatatc gtcaccgatt tgctagcaga aaatgccagc aaggtgtttg cttcaaacct
    19741 ggcagataag tttgcggtgt attcgcgtga gaagatgata tttagttcac aagcggcaac
    19801 caattgcgat attgcaacgc ttatacaaaa tgaaatcagc ggaagtgcac aagaataata
    19861 aaagctgatg acctgaaata acgccgggca tcacgcccgg cgcattaccg tctggtacag
    19921 taagctatcc agtttcttaa cgtccccttc tctactatcc ggtaacacag gcggaatttc
    19981 cccacgcgcc agttccgcat ggataaacgc atgtagcaat ggacggcgtg ggccatattc
    20041 cgcttcaccc gcacgctgtt tacgttccag taattcatct atttctgcgc gtaaggccgc
    20101 atccaactca cttcccgcca gcaattcgga aaaacgcatt ggcggtacgc cttttcccgc
    20161 ttcaacccaa cgcacggcca gcaacggacg cagcacgtaa aagtattttt tcagtcgaac
    20221 ttcatcgccc tgcaaatatc cgcggaagtt tttctgcgcc atcgaatagt aatgccagcg
    20281 cgcacgcaac ggagaaaacc acgttgggac cattgcctta agcgccgtta tcgtctcttc
    20341 atcttgctga tagaccaccg gtgaatccag ccattcaatt agcgtgggat tcgcaccttt
    20401 cagcaatccc agcgctttgc gccattccca accggaaaca tctaactcgt cgtcgatggg
    20461 cagttcgata acatcgcgtg gtgactctac ccgcagatac cattccagcg gatggacata
    20521 caaaaaccgc acatcgtaat cgctgtcggg cgaagcaaat ccccagccgc gactccccga
    20581 ttcgcaggcg tacagcactt tcacgccata gcgacgttca atttctttta actgctgcga
    20641 taccctttcg cgcatcgcag cactgactcc atttaatggc atgttactca ccctttgata
    20701 ttaaccaacc cgtgcagaac ctgatccaga ccgccaaata cagagatgtt atcaatccgc
    20761 tcagccactc tctccagcgt ttccagctct tttaaacgca acgctaccgg attgttctcc
    20821 atgactttgg cggtgttcag taacgagcgc gtcgctgccg tttcttcacg tcggcgaatc
    20881 acattcgcct gcgcagattt ctctgcctca accagctgcg cgaggatatt tttcatatcg
    20941 ccaggcagga cgatatcttt tacccctaat gaagcaatct ccataccaaa tggcaacatg
    21001 cgcgatttca cctgttcgct gacaacgtca tcaatgacct gtttatcttc cagcaattca
    21061 tccagcgtac gcgtacctac cacttcacgc agtgcaaatt gcaattcccg atacaaatga
    21121 tcaatcggct tcgttaactg actaaatgct aataacacgt cgctgtagcg ccagttagcc
    21181 gcgaggttaa tccgcagatt gaccttatct ttcgttaaga tttcttgccc gctgacttcc
    21241 agtacctgca agcgggtgtc aaccacttca gcttcaacca gatggttaat tttccagtac
    21301 gcggtaagcc ccggcggtaa cagcgcctgc gtttcaccat cgattttcag cacgcctaca
    21361 tgccaggccg gaacctgcac cgtcagtatc gcatcgcggc ctttaacaac tcctttgcgc
    21421 cgtggctgta ataccgcatt catcacctcc gtttgtaccg ggacttgctg cgtattcatc
    21481 cgcaccagcg tcaaatcatc ctctacacgc cagtacaaac gacgcgttga tggcggcagg
    21541 atctccaaca aaataccgtc catatacaac gccccagctt cagtctccga caaatctgcc
    21601 accacgcaat atctttccac ccaatcaggc tgaaagcggc gcaaataatc cgccagcacg
    21661 tcgggaactt cgctaccatc cagcgtaatc agcaacactt ccggggtatt taaccagggt
    21721 aaaatatgct cgcctgcatg cagcactttg tagtaatcgc cgtttctgga gagtaatgcc
    21781 agctgatctt ttcttacgct tattttttta atcattatct attccttgct taatagccgt
    21841 cgcggaagca gacacgaagc gacaggggaa ttcccttttg cctgttaccc ggcatcttta
    21901 tcggcgacgt tatcggggac cgcagactgc gcaatccctc attttttatt taataagtta
    21961 caaccagtat tcatttcagg aatgaatgtg ggaatcgaac ccgtaattgg cgtcaaaggc
    22021 tgacggaaca cgcccggaaa acagcctggc cgttcctggt gtgccgacgg ggtttaaccc
    22081 cctttgtaat tcagtatcaa cgcaaaagtg ttattgcgag aagcgtgcca gaagtgataa
    22141 aaaattttaa ttaca
//
LOCUS       DAPGRN010000081        21705 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPGRN010000081 DAPGRN010000000
VERSION     DAPGRN010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.081"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            233..385
                     /gene="mokC"
                     /locus_tag="RHW33_003182"
     CDS             233..385
                     /gene="mokC"
                     /locus_tag="RHW33_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV1646660.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(489..1619)
                     /gene="dnaJ"
                     /locus_tag="RHW33_003183"
     CDS             complement(489..1619)
                     /gene="dnaJ"
                     /locus_tag="RHW33_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV1646661.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAVRGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1708..3624)
                     /gene="dnaK"
                     /locus_tag="RHW33_003184"
     CDS             complement(1708..3624)
                     /gene="dnaK"
                     /locus_tag="RHW33_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV1646662.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4001..4405
                     /locus_tag="RHW33_003185"
     CDS             4001..4405
                     /locus_tag="RHW33_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV1646663.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGIIAMPHNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4431..5144
                     /gene="msyB"
                     /locus_tag="RHW33_003186"
     CDS             4431..5144
                     /gene="msyB"
                     /locus_tag="RHW33_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV1646664.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVRAVSGSAYRVT
                     IPAVLQIACLRRIVSATQV"
     gene            5293..5859
                     /gene="satP"
                     /locus_tag="RHW33_003187"
     CDS             5293..5859
                     /gene="satP"
                     /locus_tag="RHW33_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV1646665.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5894..6481)
                     /gene="mog"
                     /locus_tag="RHW33_003188"
     CDS             complement(5894..6481)
                     /gene="mog"
                     /locus_tag="RHW33_003188"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV1646666.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6596..7549)
                     /gene="tal"
                     /locus_tag="RHW33_003189"
     CDS             complement(6596..7549)
                     /gene="tal"
                     /locus_tag="RHW33_003189"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV1646667.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7828..9258
                     /locus_tag="RHW33_003190"
     CDS             7828..9258
                     /locus_tag="RHW33_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV1646668.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSRDAWDDVSQE"
     gene            9328..10104
                     /gene="yaaA"
                     /locus_tag="RHW33_003191"
     CDS             9328..10104
                     /gene="yaaA"
                     /locus_tag="RHW33_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV1646669.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10257..10553)
                     /locus_tag="RHW33_003192"
     CDS             complement(10257..10553)
                     /locus_tag="RHW33_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV1646670.1"
                     /translation="MKKMQSIVLALSLVLVAPMATQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(10767..12053)
                     /gene="thrC"
                     /locus_tag="RHW33_003193"
     CDS             complement(10767..12053)
                     /gene="thrC"
                     /locus_tag="RHW33_003193"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV1646671.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12054..12986)
                     /gene="thrB"
                     /locus_tag="RHW33_003194"
     CDS             complement(12054..12986)
                     /gene="thrB"
                     /locus_tag="RHW33_003194"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV1646672.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCHELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDSRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12988..15450)
                     /gene="thrA"
                     /locus_tag="RHW33_003195"
     CDS             complement(12988..15450)
                     /gene="thrA"
                     /locus_tag="RHW33_003195"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV1646673.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15476..15595)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15531..15596)
                     /gene="thrL"
                     /locus_tag="RHW33_003196"
     CDS             complement(15531..15596)
                     /gene="thrL"
                     /locus_tag="RHW33_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV1646674.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15810..16496)
                     /locus_tag="RHW33_003197"
     CDS             complement(15810..16496)
                     /locus_tag="RHW33_003197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV1646675.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16896..17036)
                     /locus_tag="RHW33_003198"
     CDS             complement(16896..17036)
                     /locus_tag="RHW33_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646676.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17132..17848
                     /gene="arcA"
                     /locus_tag="RHW33_003199"
     CDS             17132..17848
                     /gene="arcA"
                     /locus_tag="RHW33_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV1646677.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17907..19259)
                     /gene="creD"
                     /locus_tag="RHW33_003200"
     CDS             complement(17907..19259)
                     /gene="creD"
                     /locus_tag="RHW33_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV1646678.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19317..20741)
                     /gene="creC"
                     /locus_tag="RHW33_003201"
     CDS             complement(19317..20741)
                     /gene="creC"
                     /locus_tag="RHW33_003201"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV1646679.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20741..21430)
                     /gene="creB"
                     /locus_tag="RHW33_003202"
     CDS             complement(20741..21430)
                     /gene="creB"
                     /locus_tag="RHW33_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV1646680.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21443..>21705)
                     /locus_tag="RHW33_003203"
     CDS             complement(21443..>21705)
                     /locus_tag="RHW33_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HDV1646681.1"
                     /translation="AEDTSDAAISCQQVGPIELSDRIKNGKAQGEVVFKKRTSLVFKS
                     LQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         5177 a         5795 c         5306 g         5427 t
ORIGIN      
        1 attacaccat aaaaatgcga ggcgtcttcc ggattagaat cgataaaaca gaatgggtta
       61 aagagagcga tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac
      121 ggcagataga gaaaagcccc gagtgatatt ttaccatcaa cccgaggccc cctatatgct
      181 gaacacatgt agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca
      241 gcataaggcg atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct
      301 ggtaacgaga aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt
      361 tttcacggct tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca
      421 ggtgctcgca tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt
      481 tggggaggtt agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt
      541 gagcgcgggc tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc
      601 agctgtttct gcttctcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagcaaa
      661 tcaccctgtg cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaacagctta
      721 ccggtctggg tttcgccagg cactttcagt ttaacgcgac catcaagggt cggtacttcg
      781 atttcaccac ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg
      841 ccttcacgct cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc
      901 ggtgcgccgt gttcacccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc
      961 acccctgccg ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga
     1021 catttgttgc acggatcttt gatcagcgta ccgcggccct ggcagtgtgg acaggtctgt
     1081 tgcacggcaa agaaaccctg gcgcatctgc acctgaccag agccatgaca ggtcggacag
     1141 gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga
     1201 gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg
     1261 ttatagcgta aatcagcacc gcgcaccgca cgttgacgac cacgtccgcc gccaaaaata
     1321 tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accgccgccg
     1381 cccatgccac cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc
     1441 gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg
     1501 tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttttgatt
     1561 tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc
     1621 ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt
     1681 cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc
     1741 gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg
     1801 ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagttcctg
     1861 cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag
     1921 cgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg
     1981 cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa
     2041 cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc
     2101 gttcagacca gaagacgcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt
     2161 cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat
     2221 gccgcgcggt gccgggttga taccatccag gttgaactga cccagagatt tgttatcagc
     2281 cgcacgttta cgttcaccct gcaacacatg gatggttacc gcagactggt tgtcttcagc
     2341 ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt
     2401 catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac
     2461 gtcttttacg tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc
     2521 gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac
     2581 cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag atacagacag
     2641 gccagcgtcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac
     2701 caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcgtc
     2761 tgcagtgatg tacggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc
     2821 tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc
     2881 ctgatctttc ttgaattctt caaccagata gttgatcaga cggctgtcga agtcttcacc
     2941 acccaggtgg gtatcaccgt tggttgccag aacttcgaag gttttttcgc cgtcaacttc
     3001 gtcgatttca ataatagaaa tatcgaaagt accaccaccc aggtcataaa ccgcgatagt
     3061 acggttgcca gtacctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat
     3121 gatacgtttt acttcgagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc
     3181 atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac ccaggtaatc
     3241 ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg gtgccatttt
     3301 ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat
     3361 gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat
     3421 cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag
     3481 agtttcacca tcctgggtat aggcaataat agaaggcgtg gtgcgatcgc cttcggcatt
     3541 ctccagcaca cgaggagtgg tgccatccat aatcgctaca caagagttgg tagtacccag
     3601 gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg
     3661 ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc
     3721 agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag
     3781 atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatggcagga caagacgtaa
     3841 aattgacagc aattttattc atataaagaa tgaatcgcag ccaaggtatt catcaaaaaa
     3901 ttgtaataaa aagaaaagat tacgtgcctg aatcttctct ttatcagcag taaactagtg
     3961 ggtattcatc cccctacctc ttcccactaa gagaatcctt atgaaatccg tttttacgat
     4021 ttccgccagc ctggcgatta gcctgatgct gtgctgcacg gcgcaggcaa acgaccataa
     4081 actcctcggc atcattgcaa tgccgcataa cgaaaccaac gatctggcgc tgaaacttcc
     4141 tgtttgtcgc attgtgaaac gcatacaact ctctgccgac catggcgatt tacagttaag
     4201 cggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc
     4261 agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa
     4321 acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac
     4381 gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta
     4441 attacctgaa tgattcagat ctggattttc tccagcattg tagtgaggaa cagttagcaa
     4501 atttcgcccg actgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcgtactga
     4561 tgcgcaacga actgtttaaa tcgatggaag gacatcccga gcaacatcgc cgcaactggc
     4621 agctgattgc cggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg
     4681 gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaagcgattg aagctgaaag
     4741 ccgacaaaga gatgtctacg tttgaaattg agcaacagtt actggaacaa tttctgcgta
     4801 atacctggaa gaaaatggac gaggaacata agcaggagtt tctgcacgcg gtcgatgcca
     4861 gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag
     4921 gtgtgtcgca tttgctttcc agccaattga cccgaatttt acgcacccac gcagcaatga
     4981 gcgtgcttgg gcatggtttg ctgcgcggcg cgggtctggg aggccctgtg ggcgcagcat
     5041 taaatggggt tagagcggtc agcggcagcg cctatcgcgt gacgattcca gccgtactgc
     5101 aaatcgcctg cctgcgccgg atagttagcg ccactcaggt ctgataccgg aaaccgggga
     5161 aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc
     5221 gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat
     5281 ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc
     5341 ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt
     5401 attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg
     5461 gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg
     5521 ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca
     5581 cagttccttg gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc
     5641 actctgaaag gcgcacgcgt cctgcaattc gttttcttta gcctgaccgt gctgtttgcc
     5701 ctgctggcga tcggtaacat tgccggtaac gccgcaatca tccactttgc cggctggatt
     5761 ggtctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtact gaacgagcag
     5821 tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgttttgaga tccccccgct
     5881 cggggggatt tttttattcg ctaacttcgc gtcttgcact cttcggtctg aatgcagcaa
     5941 ccacttctgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc
     6001 tggcaaaaat accgtgtacc acaacgttac cctcagcgtc cttcacacct tccagcgtct
     6061 ctttaataga cttcggctga ccaggtaagt taaggatcag cgcctgtttg cgaatcacac
     6121 ccacctgacg cgaaaggatc gcagttggta caaaatgcag gctgatctgg cgcatctgtt
     6181 caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac
     6241 gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacca
     6301 gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt
     6361 caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgcagggata cctttatcct
     6421 gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca
     6481 tcaagatttt tccgtttaaa cagtctcgtt aaacagaatg atacactgcg aagggagtga
     6541 cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca
     6601 gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt
     6661 ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag
     6721 tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc
     6781 cttcgctctc agccagctct ttcagcagtg ccggtgcgat ggtcagacgg tcgcagcctg
     6841 ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac
     6901 cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg
     6961 gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg ccaacaaatg
     7021 gagagatcag aaacacgccc gcttccgcac aagcacgagc ctgagcgaag gagaacagca
     7081 gggtcaggtt acagttgatg ccttcttttt ccagctgttc cgcagcacgg ataccctgcc
     7141 aggtagaagc cagtttgatc agaatacgat cgttgctaat acctgcatcg ttgtagagtt
     7201 tgatcaggcg ttttgctttc gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa
     7261 cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata tttactgcca
     7321 gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg
     7381 cgacagcatc atcaatcaac ttacgatatt ccggaatctg cgctgcgtta agaatgagag
     7441 aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt
     7501 cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc
     7561 tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac
     7621 gtcagcgcaa ccgccgacaa gatggttatc gccaggcgag actgtttcgg atttctgaca
     7681 ctgcgttgat atcgcccgcc atttttaaac aaaacctcat gtatgctacg cagaagttat
     7741 caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca
     7801 tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc
     7861 ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc
     7921 accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt
     7981 catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc
     8041 gtgggtagcg gcaacctggc cggcgttgcg ctggctatta ccgccggtgg acctggagcc
     8101 gtcttttgga tgtgggttgc cgcgtttatc ggcatggcga cctcgtttgc cgaatgttcc
     8161 cttgcacaac tttataaaga acgtgacgtc aatgggcagt ttcgtggcgg accggcatgg
     8221 tatatggcgc gcgggctggg gatgcgctgg atgggcgttc tgttcgccgt ctttttgctc
     8281 atcgcctacg gcataatttt cagcggagtt caggcaaacg ctgttgcccg agccctgagt
     8341 ttttcttttg attttccccc gctggtgaca ggcattattc tcgctgtctt tgctctgctg
     8401 gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg
     8461 gcgataatct gggtactgac cagcctggtg atttgcgtaa taaatatcgg gcaacttccc
     8521 cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg
     8581 gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat
     8641 gaggcgggaa tgggttcgac gccaaacgcg gcagcggcag cggcgtcctg gcctccgcat
     8701 ccggcagcgc aaggaattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt
     8761 acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat gccgctggaa
     8821 ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctgggg tgctgagttt
     8881 gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc
     8941 gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc
     9001 tgcaccttcg caacggtcat cggcggcacc ttgctaagtc ttccgctgat gtggcaactg
     9061 gcagatatca taatggcctg catggctatt accaatttga ccgccatttt actgctctcg
     9121 cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg
     9181 gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctcggga cgcgtgggat
     9241 gatgtttcgc aggagtaatc acaactatcg atcaactcat tctcgttttt tgctaaagtc
     9301 ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt
     9361 gattaccaaa gcccgttgac caccacgcga tatacactgc cggagctgtt agacaattcc
     9421 cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgt
     9481 atcagcgaca aactggcagg tatcaacgcc gctcgctttc atgactggca gccagatttc
     9541 acgccggaga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg
     9601 caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt
     9661 tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg
     9721 gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc
     9781 accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg
     9841 tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg
     9901 gtgttcctcg atgagaagaa cggcaaattt aagataatca gcttctacgc taagaaagca
     9961 cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga gcaactgact
    10021 ggttttaata gcgaaggtta cttctttgat gaagcttcct ccagcaatgg cgaactggtg
    10081 tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt
    10141 atccggccta caaaagcatg cagattcaat atattgcaga attcacgtag gcctgataag
    10201 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
    10261 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gatgagcttt cttatggtga
    10321 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
    10381 ttccaccagc cgtggtcgcg ccagtgaccg ccatcccagt aatagccacg attatcacga
    10441 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg cgttgccatg
    10501 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
    10561 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacggcggt
    10621 tgttattctc cgcaatccta atctctaagt taccgcattt attgagaatt tctccttttt
    10681 ccctgtcgtt tctctccata ttttcttcac aaaaaagccg ggctgcataa aagcaaaccc
    10741 ggcctgattg agataatgaa tagatgttac tgatgattca tcatcaattt acgcaacgca
    10801 gcaaaatcgg cgggcagatt atgcgaaagc aagggtaaat cggcacgttc tgccagctct
    10861 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
    10921 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
    10981 tacgctacgg cagcgtgcgg ctccgaagtg tagcccagtt cttttaactc acgcattgtc
    11041 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
    11101 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
    11161 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt catgcaggaa acgtggcacg
    11221 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgag tgacttcgcc
    11281 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
    11341 cgcgcttcct gcggcagctg cgcaactgct tcaaagtagt agcaaatctg cgccagcaaa
    11401 cggctgatgt taatcgagtt agctgagttt aaccccagcg ccactttcag ctcttcatca
    11461 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
    11521 tcgatattgc cgcccaatgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
    11581 ggatagagga taaccacttt cacattcggt aaaccgtaga aagcatgagc cactgccgct
    11641 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcgcccgc aatatgggtc
    11701 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
    11761 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
    11821 gcgcgcacgc gttcttccag gatttcctgc gggatttcat caccaataaa cgccgagagg
    11881 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
    11941 aattccggca ggtcgtgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
    12001 gcttgcgcaa agctgacctg ctcattgtga tctttcagat tgtagagttt catttagttt
    12061 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
    12121 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacac
    12181 agagcgaaca aggtcgggcc ggagccggag ataccgctcg ctaccgcgcc gatttccgcg
    12241 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttctgcgata
    12301 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
    12361 cctgccagat gtcgcccgtg cgcaatgcaa tcctgacggc gatactgcgc cggtaaaata
    12421 gcccgggctt ctgccgtcga gactttaatc cccggatacg ccagcaccca cagccactca
    12481 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
    12541 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagattcg tccttccagc
    12601 tcgcccatca aagccagcaa acgagagtca ttaagcggct tgccacagtg ttcattcatc
    12661 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gatcggcata
    12721 ttcttttcca gagtcatcgc cactggaatt tgcttgccca gctcatggca aaaacgctcc
    12781 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
    12841 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
    12901 ccatcaacgg gtgtcaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
    12961 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
    13021 cagatcggca aagacaccgg cagcggtaac gtcattgccc gcaccatatc cgcgcagtac
    13081 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
    13141 tttgaacagc ggatcattac catccacttc ggcaatcttc acgcggcaga caccatcttc
    13201 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
    13261 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcggc
    13321 gttaaactct gcgggcagca ctggttcaat ttcaatatcc gccaactcca gttcacgtcc
    13381 cgtttcacga gcgaggatca atagtttacg cgccacatcc ataccagaaa gatcatctcg
    13441 cgggtccggt tcggtataac ccatttcccg cgccagcgta gtcgcctcgg agaaactcat
    13501 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
    13561 catcaattca tcacctgcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
    13621 cccaacgttg gtgtcataga ggaatttacg tcgcgatttt tccgccgcat aacgcaactg
    13681 atggtagtaa tccatcgacg aggtgttggc ctttttattc ggcgtgacaa cgtggaaacc
    13741 ttcgcgcagg aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
    13801 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
    13861 ctctttggct tgtgcaagtt cttcctgcca gttttccaga ttaaggccat gcacattggt
    13921 gagcagagcc ttcgagttag caacaccgca gacacgtaag tcgatatgtt tattcttcaa
    13981 ccagctttgc tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
    14041 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
    14101 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
    14161 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
    14221 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
    14281 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
    14341 agctcgcaca cagtcgcttt gcggaacgca gaaactgata ctgtattcgg aagatgattg
    14401 cgtaatcagc accacggaaa tacgggcgcg tgacattgca gcaaagacgc gcgccgccat
    14461 gccgaccatt cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
    14521 ggaaatgccc ttgaccggta attcgtcttc atcacggctg gcaccaatta gcgtacctgg
    14581 agcttgagga tttccggtat ttttaatcag gcacgggatc tggaactggg cgatgggggt
    14641 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaagaa agctccatcg cttcctggta
    14701 ggacatcgat ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatatacccc
    14761 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
    14821 ggagtagtcg gaaccgttgc gtccaagtac caccagttcg cctttttcat taccggcggt
    14881 gaaacctgcc atcagcacca tgtgatcagc cggaatacga cttgccgcaa tacggcgggt
    14941 ggactctgca atatcgacag tagattcgag gtaatgcccc actgccagca gtttttcgac
    15001 cggatcgata acggtaacgt tgtgaccgcg cgcttctaat acgccggcca taatggcgat
    15061 cgacattttc tcgccacggc aaatcagcgc agcgttaatg ctatccgggc actgccctaa
    15121 caaactaatg ccatgcagga catgttttat ttgagcaaat tcctggtcga cgaaagtttt
    15181 caattgcgcc agcgggaatc ccggctgggc ggcggcgagt cccgtcagaa gttcggcaaa
    15241 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
    15301 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
    15361 ctgcctggca ttgctttcca gaatatcggc aacccgcaga aaacgttctg catttgccac
    15421 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
    15481 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
    15541 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
    15601 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
    15661 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
    15721 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
    15781 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
    15841 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgttgtaac
    15901 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catggctcgt
    15961 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgt cggttgttgt
    16021 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
    16081 taatccgcca ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc
    16141 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
    16201 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactgcgc
    16261 gcagtggtgg cgacagtgaa atcgacatcg tgtaacgatt cagccagcgt cgggaaaact
    16321 ttaatattat caataatatc accagatcca tgtgcgaccc agcgagtggc tggctccaga
    16381 tgtgcctgac tatcgacaat ccgcagttcg ctaaacccca tcgttttcat tgcccgcgcc
    16441 gctgccccaa tattttctgc tctggcgggt gcgaccagaa taatcgttat acgcatattg
    16501 ccactcttct tgaccaaatg accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
    16561 cgaatttaca aattcttaac gtaagtcgca gaaaaagccc tttacttagc ttaaaaaagg
    16621 ctaaactatt tcctgactgt actaacggtt gaattgttaa aaaatgctac atatccttct
    16681 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
    16741 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
    16801 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
    16861 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
    16921 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
    16981 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
    17041 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
    17101 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
    17161 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
    17221 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
    17281 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
    17341 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
    17401 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
    17461 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
    17521 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
    17581 tttgatcggc cctgatggcg agcagtacaa gctgccgcgc agtgagttcc gcgccatgct
    17641 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
    17701 cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
    17761 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accattcacg gtgaaggtta
    17821 tcgcttctgc ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aacggcgctt
    17881 tttagcgccg tttttatttt tcaaccttat ttccagatac gtaattcatc gtccgttgta
    17941 acttctttac tggctttcat tttcggcagt gaaaacgcat accagtcgat attacgggtc
    18001 acaaacatca tgccggccag cgccaccacc agcacactgg ttcccaacaa cagcgcgcta
    18061 tcggcagagt tgagcagtcc ccacatcaca ccatccagca gcaacagcgc gagggtaaac
    18121 aacatgctgt tgcgccaacc tttcaatacc gcctgcaaat aaataccgtt cattaacgcc
    18181 ccaatcagac tggcgattat ccatgccacg gtaaaaccgg tatgttcaga aagcgccagc
    18241 aagagcaaat aaaacatcac taatgaaagc cccaccagca aatattgcat tgggtgtaag
    18301 cgttgcgcgg tgagcgtttc aaaaacaaag aacgccataa aagtcagcgc aatcagcaga
    18361 atggcatact tagtcgcccg gtcagttaat tggtattgat cggctggcgt cgtcactgca
    18421 acgctaaacg cggggaagtt ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg
    18481 agattattag caaaccagct gctttgccat tgcgcctgaa aacctgactc actaacttcc
    18541 cgtttggctg gtagaaaatc acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc
    18601 atttcgctat tacgccccgc aggcaccaca gaaagatcgc cggtaccgct taaattcagt
    18661 gccatattca gcttcaggtt ctgcttccgc cagtcccctt cgggtaaagg gatatgcacg
    18721 ccctgcccgc cttgctctaa cccggtgccg ggttcaatgg tcagcgccgt tccgttaact
    18781 tcaggcgctt tcaccacacc aataccacgc gcatccccga cgctaatcac aataaatggc
    18841 ttgccaaagg tgatatttgg cgcgtcgagt tcgctaagac gcgaaacatc gaaatcggct
    18901 tttagcgtta aatcactgtg ccagacctga ccggtataaa tccctatctt gcgttcttcc
    18961 acgttctgat tgccatcaac catcaatgac tcaggtaacc aaaaatggat aaaacttcgt
    19021 ttccgctcca cggttttatc ctcttcctgt accgtataaa gctcggtcac aggaatagcg
    19081 ataagcggcc caacgagttt ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca
    19141 tcgctacggt aatcggcacg ttcgacaatc acctgccgaa tcagcattat cggaatcaac
    19201 aacagcaata cggcaccaaa cagggtggtc attttccaga acagggggga tttcaacata
    19261 gtcttctcct ttgcaatggc agcaggatac gaaggctatg tgggaagaat ttgaagctat
    19321 gtgaagtgac ggtgaagtcg aagcgaggcc agcacgccac cttcctgcac gttgcgcagc
    19381 gtgacttcgc cgttaaacaa acgggcgacc tcactgacga acgccaaccc cagaccgctg
    19441 cttttttgcc catttgcacg cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg
    19501 tagtcaggaa taccactacc ggtatccagc accttaaggg cgacgtgttc ctgatccact
    19561 tcggcgctta gcgttatgcg accgctctcg ggggtaaaat cgatggcgtt atccagtaaa
    19621 ttccccagcg cctgctccag taacgccggt tcagcagcaa cgttaacctc agtaggcata
    19681 acatgcaaag tgatgttttt ttctgccaac tgcacggtgc gcgcttcgct gacgcggcga
    19741 aataatgccg ccacatcaac agcagtcaga acgacttcct gacgattctc cagtcttgcc
    19801 tggcgtagta acgtttctac caatgcctgc atacgcgcat tttgcgtcag aatgttgtca
    19861 gtaaaacgag ccactacttc aggcggcgga ccttcgcgta aaatttccgc cgcgccacga
    19921 atcgccgcca gtgggctttt tagctcatgg gttaacgcat aaacatactg ctcaatatag
    19981 tttttccctt ccagcttcac gcgcatactt tccagcgcct gcgcaagctt acgcaactcg
    20041 ctactgccga gttcaggaag aggaacgggc ttattgtcag tgacggaatc ggcatagcga
    20101 gtgagccggg caatggagcg attgatccac caaaccatgc ctgcgccaat caccagtgca
    20161 atccccagca aaatggcgct ggcccataaa attcgccgct cgctgcgctt aatgaccgga
    20221 gccatcgccg cgttcggttt gcctacgctc aaaacgccaa taagccgcga gccgtccata
    20281 atcggcgcgg caacatacat caccgaactt tcgggatcgg caggattttg caacgtgctg
    20341 cgcgcaccat actgaccacg caacgttagc cagacgtcat tccagcgcga ataatcctgt
    20401 ccaacggctt tatttgccga atcgaacaat actttgccct gcgcatcggt catatagaca
    20461 tgatattcat tgcgcacttt gttaatgcca ccgatattgg cgcgaaacgg gcgatgttgt
    20521 agctgattaa acgcctgcgc cagttgccca tgcgttgggt ccccagagag caaatcggga
    20581 cgcgccagct ccgccagcaa cgttgcggtg tcgatcagcg tcccctccgt tgctcttcgc
    20641 acgcccggtt taacttcttt gacaaaaata gccagtacga accaggccgc caccgccacc
    20701 agtaaaaaat agcccagcaa caaccgcatg ccgatacgca ttacaggccc ctcaggctat
    20761 atcccatgcc gcgatgagta ttaatcggtg aaagatcggg gttgatggcg cgcagcttgg
    20821 cacgcaacgt tttaatgtgg gtatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc
    20881 acacgctatc cattagttgc tggcgggacc agacgcggcc tggcgacttg agtaacgtct
    20941 tcagcaataa aaactcatac cgcgtcagcg ttaatggcgt gtcaaaccag ctgatttgcg
    21001 ccgcgggttc attcaattca aaatgaccaa tacggatgac gggagacggc gacgagaact
    21061 tcttcacccg acgcagtaag gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt
    21121 tagccacata gtcgtcagcg ccaatttcca gcccaagcag gcgatcgacc tcttcacttc
    21181 gggccgtcag gaacagtaca ggtaacgccg gatggagcgc cagtaactgg cggcacaatt
    21241 caaagccact gatatcaggc aaaccaacat cgagaatcat gacgtcggga acctgctggc
    21301 gagctttatc cagcaccggc aaacctcgct caaagacctc gacggcaaaa ccttcctgct
    21361 gcaacatgta gaccagcgtg tcggctatcc cttgctcatc ttccactaac cagaccgttt
    21421 cccgttgcat aaatcgcctc tgttattgcc gccacggcat gacaggaacc gcgctaatcg
    21481 cgtttttcgg cgaaccttct acaactttgt cggagtaagc cagataagcg agcgcgttgc
    21541 gtttggcgtc ataaaagcgc acgacctgta acgacttaaa gaccagggac gtgcgttttt
    21601 tgaatactac ctcgccctga gctttgccgt ttttaatacg atccgacagt tcaatcggcc
    21661 cgacttgctg acaagaaata gccgcatcgg aggtatcttc cgcca
//
LOCUS       DAPGRN010000082        21485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPGRN010000082 DAPGRN010000000
VERSION     DAPGRN010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.082"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            130..1644
                     /gene="caiT"
                     /locus_tag="RHW33_003204"
     CDS             130..1644
                     /gene="caiT"
                     /locus_tag="RHW33_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV1646682.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1675..2817
                     /gene="caiA"
                     /locus_tag="RHW33_003205"
     CDS             1675..2817
                     /gene="caiA"
                     /locus_tag="RHW33_003205"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV1646683.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2946..4163
                     /gene="caiB"
                     /locus_tag="RHW33_003206"
     CDS             2946..4163
                     /gene="caiB"
                     /locus_tag="RHW33_003206"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV1646684.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4237..5790
                     /gene="caiC"
                     /locus_tag="RHW33_003207"
     CDS             4237..5790
                     /gene="caiC"
                     /locus_tag="RHW33_003207"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV1646685.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5899..6684
                     /gene="caiD"
                     /locus_tag="RHW33_003208"
     CDS             5899..6684
                     /gene="caiD"
                     /locus_tag="RHW33_003208"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV1646686.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALHWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6690..7280
                     /gene="caiE"
                     /locus_tag="RHW33_003209"
     CDS             6690..7280
                     /gene="caiE"
                     /locus_tag="RHW33_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV1646687.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQTGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGHDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRTVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7399..7794)
                     /gene="caiF"
                     /locus_tag="RHW33_003210"
     CDS             complement(7399..7794)
                     /gene="caiF"
                     /locus_tag="RHW33_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV1646688.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8055..11276)
                     /gene="carB"
                     /locus_tag="RHW33_003211"
     CDS             complement(8055..11276)
                     /gene="carB"
                     /locus_tag="RHW33_003211"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV1646689.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11294..12442)
                     /gene="carA"
                     /locus_tag="RHW33_003212"
     CDS             complement(11294..12442)
                     /gene="carA"
                     /locus_tag="RHW33_003212"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV1646690.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12898..13719)
                     /gene="dapB"
                     /locus_tag="RHW33_003213"
     CDS             complement(12898..13719)
                     /gene="dapB"
                     /locus_tag="RHW33_003213"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV1646691.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLHNL"
     gene            complement(13886..14800)
                     /gene="rihC"
                     /locus_tag="RHW33_003214"
     CDS             complement(13886..14800)
                     /gene="rihC"
                     /locus_tag="RHW33_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV1646692.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMADYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(14866..15816)
                     /gene="ispH"
                     /locus_tag="RHW33_003215"
                     /gene_synonym="lytB"
     CDS             complement(14866..15816)
                     /gene="ispH"
                     /locus_tag="RHW33_003215"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV1646693.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15818..16267)
                     /gene="fkpB"
                     /locus_tag="RHW33_003216"
     CDS             complement(15818..16267)
                     /gene="fkpB"
                     /locus_tag="RHW33_003216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1646694.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16355..16849)
                     /gene="lspA"
                     /locus_tag="RHW33_003217"
     CDS             complement(16355..16849)
                     /gene="lspA"
                     /locus_tag="RHW33_003217"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV1646695.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16849..19665)
                     /gene="ileS"
                     /locus_tag="RHW33_003218"
     CDS             complement(16849..19665)
                     /gene="ileS"
                     /locus_tag="RHW33_003218"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV1646696.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKELLGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCTKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19708..20649)
                     /gene="ribF"
                     /locus_tag="RHW33_003219"
     CDS             complement(19708..20649)
                     /gene="ribF"
                     /locus_tag="RHW33_003219"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV1646697.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20657..20875)
                     /locus_tag="RHW33_003220"
     CDS             complement(20657..20875)
                     /locus_tag="RHW33_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV1646698.1"
                     /translation="MCRHSLRSDGAGFYQLAWCEYSFSAIKIAAGSQFLPVICAMTMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20978..21241
                     /gene="rpsT"
                     /locus_tag="RHW33_003221"
     CDS             20978..21241
                     /gene="rpsT"
                     /locus_tag="RHW33_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV1646699.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5112 a         5771 c         5537 g         5065 t
ORIGIN      
        1 tattgaatac ccattatgag ttagccatta acgcgtccac gaggttaata ataattatat
       61 taaatgttaa caaaaataaa acaaacggga acgcaaaata aatattcgtt ttcacagtgg
      121 aattaactca tgaagaatga aaagagaaaa acgggaatag aaccgaaggt tttctttccg
      181 ccgttaataa tcgtcggcat actttgttgg cttacagtca gagatctgga tgcggcgaat
      241 gtcgttatta atgctgtatt cagttacgtc accaatgtat ggggatgggc atttgaatgg
      301 tatatggtgg tgatgctttt cggttggttc tggctggtgt ttggcccgta tgccaaaaag
      361 cgtttaggta acgaaccgcc agaatttagc accgccagtt ggatctttat gatgttcgcc
      421 tcctgtacgt ctgctgccgt actgttctgg ggatcgattg agatctacta ctacatctcc
      481 accccgccgt ttggcttaga accgaactcg acaggagcga aagagttggg gctggcttac
      541 agcttgttcc actggggacc tctgccgtgg gccacttaca gcttcctttc agtcgccttc
      601 gcttacttct tctttgtccg caaaatggaa gtgattcgcc ccagctcgac actggtgccg
      661 ctggtaggtg aaaaacacgc caaagggttg ttcggtacca tcgtcgacaa cttctatctc
      721 gtcgccttga tcttcgcgat gggtaccagt ctgggccttg ccacgccgct ggtgaccgag
      781 tgtatgcaat ggttgtttgg cattccgcat accctgcaac tggacgctat catcattacc
      841 tgctggatta tcctcaacgc catttgcgtc gcctgcggtc tgcaaaaagg ggtacgtatc
      901 gccagtgacg tgcgtagtta cctgagcttc ctgatgctgg gttgggtgtt catcgtcagc
      961 ggtgccagct tcatcatgaa ctacttcacc gattcggtgg ggatgttgct gatgtatctg
     1021 ccgcgcatgt tgttctatac cgatcccatc gctaaaggcg gcttcccgca gggctggacc
     1081 gtgttctact gggcatggtg ggtgatttac gccatccaga tgagtatctt cctcgcccgc
     1141 atctcccgtg gtcgtactgt gcgtgaactg tgcttcggca tggtgatggg gctgacagca
     1201 tccacctgga tcctgtggac cgtgctcggt agtaacactc tgctgttgat agataaaaac
     1261 atcatcaaca ttccaaatct gatcgaacag tacggtgtgg cgcgcgccat catcgaaacc
     1321 tgggccgctc tgccactcag caccgccacc atgtggggct tcttcatcct ctgctttatt
     1381 gccaccgtta cgctggttaa cgcctgctct tataccctgg cgatgtccac ttgccgcgaa
     1441 gtacgcgatg gtgaagagcc gccgctgctg gtgcgtatcg gttggtcaat tctggttggc
     1501 attatcggta ttgttctgct ggcgctcggc ggcctgaaac cgattcaaac cgccattatc
     1561 gccggaggat gcccgctgtt cttcgtcaac attatggtga cgctctcctt tattaaagac
     1621 gcgaaacaga actggaaaga ttaattaacc cccaaaatat caagaggttg aaagatggat
     1681 tttaatttaa atgatgagca ggaactgttt gtcgccggta tccgcgaact gatggccagc
     1741 gaaaactggg aggcctattt tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt
     1801 gtcaaagcac tggcggatat gggtatcgac agtctgctga tccctgaaga gcacggtggt
     1861 ctggacgcgg ggtttgttac tctcgccgcc gtgtggatgg agctgggacg tctgggggca
     1921 ccaacctatg tactgtacca gctgccgggc gggttcaata ccttcctgcg cgaaggcaca
     1981 caagagcaga tcgacaagat tatggctttc cgcggcaccg gtaagcagat gtggaactca
     2041 gcgattaccg aaccgggcgc gggctccgac gtgggtagcc tgaaaacgac ttatacccgt
     2101 agaaatggta agatttatct taatggtagt aagtgtttta ttaccagtag cgcctacacc
     2161 ccgtacatcg tggtgatggc gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa
     2221 tggtttgttg atatgagcaa accgggcatc aaagtgacca aacttgagaa gctcggtctg
     2281 cgtatggata gctgctgtga aatcaccttt gacgatgtgg aactggacga gaaagacatg
     2341 ttcggtcggg aaggtaacgg ctttaatcgc gtcaaagaag agttcgacca tgaacgtttc
     2401 ctggtagccc tgaccaacta cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac
     2461 gccaaccagc gcgtgcagtt tggcgaggct attggtcgtt tccagttgat tcaggaaaaa
     2521 ttcgcccaca tggcgatcaa attaaactcc atgaaaaaca tgctgtatga agcagcgtgg
     2581 aaagcagaca acggcaccat cacctctggc gatgcagcga tgtgcaaata cttctgcgcc
     2641 aatgcggcat ttgaagtggt ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg
     2701 ggcaaccacc gcatcagccg cttctggcgt gacctgcgtg tagaccgcgt ctccggggga
     2761 tctgacgaaa tgcagatcct gacgctgggt cgcgcggtgc tgaagcaata ccgctaagtc
     2821 cgtgcgctgc ctgatgcgac gctaatgcgt cttatcaggc ctacgggagc acaaatgtag
     2881 gccggataaa gcgtttacgc cgcatccggc agtcatgcgc tacataaatt tttcaggagc
     2941 taataatgga tcatctaccc atgccgaaat tcgggccgtt ggccggattg cgcgttgtct
     3001 tctccggtat cgaaatcgcc gggccgttcg ccgggcaaat gttcgcagaa tggggtgcgg
     3061 aagttatctg gatcgagaac gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc
     3121 cgcagctctc ccgccgcaat ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc
     3181 gcgaagcgtt tctgaaatta atggaaacca ccgatatctt catcgaagcc agtaaaggtc
     3241 cggcctttgc ccgtcgtggc attaccgatg aagtactgtg gcagcacaac ccgaaactgg
     3301 ttatcgctca cctgtccggt tttggtcagt acggcaccga ggagtacacc aatcttccgg
     3361 cctataacac catcgcccag gccttcagtg gttacctgat tcagaacggt gatgttgacc
     3421 agccaatgcc tgccttcccg tataccgccg attacttttc tggcctgacc gccaccacgg
     3481 cggcgctggc agcactgcat aaagcgcgtg aaaccggtaa aggcgaaagt atcgacatcg
     3541 ccatgtatga agtgatgctg cgtatgggcc agtacttcat gatggattac ttcaacggcg
     3601 gcgaaatgtg cccgcgcatg agcaaaggta aagatcccta ctacgccggt tgcggtctgt
     3661 ataaatgtgc cgacggctac atcgtgatgg aactggtggg cattacccaa attgaagagt
     3721 gctttaaaga tattggcctc gcacatctac tcggtacgcc ggaaatcccg gaaggcactc
     3781 agcttatcca ccgtatcgaa tgcccttacg gcccactggt agaagaaaaa ctcgatgcct
     3841 ggctggcggc acataccatc gcagaagtta aagaacgctt tgccgaactg aatatcgcct
     3901 gtgccaaagt actgaccgta ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat
     3961 caatcactca gtggcaaacg atggatggtc gcacctgcaa agggccgaac atcatgccga
     4021 aattcaaaaa taaccccgga caaatctggc gcggaatgcc ctcacatggc atggacacgg
     4081 ctgccatttt gaaaaatatc ggctacagcg aaaacgacat tcaggagttg gtcagcaaag
     4141 gtctggccaa agttgaggac taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg
     4201 cccaataaca aacgaacaaa aatggataga ggtgcaatgg atatcattgg cggacaacat
     4261 ctacgtcaaa tgtgggacga tctggcggac gtttacggtc ataaaacggc gctgatttgt
     4321 gaatccagcg gcggagtcgt taaccggtat agttatcttg agttaaatca ggagattaac
     4381 cgcacggcaa acctgtttta tacgctgggg attcgcaaag gcgacaaggt tgcactacat
     4441 ctcgacaact gcccggaatt tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt
     4501 atggtgccga ttaacgcccg cctgttacgc gaggaaagcg cgtggatcct gcaaaatagc
     4561 caggcgtgcc tgctggtgac cagtgcgcaa ttctatccta tgtatcaaca gattcagcag
     4621 gaagatgcca ctcaattgcg gcacatttgc ctgacagatg tggcacttcc cgctgatgat
     4681 ggcgtgagtt cgtttactca actgaaaaat caacaacctg ccaccttgtg ctatgcaccg
     4741 ccgctatcga ctgacgatac ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg
     4801 aaaggtgtgg tgattaccca ttacaacctg cgcttcgctg gatattactc cgcctggcag
     4861 tgtgcactgc gtgacgatga cgtctacctg acggtaatgc ctgcgtttca tatcgattgc
     4921 cagtgtactg cggcgatggc ggcgttttct gccggggcca cctttgtgct ggtcgagaaa
     4981 tacagcgccc gcgccttctg gggacaggta cagaagtacc gcgccaccat taccgaatgt
     5041 attccgatga tgattcgtac gttgatggtg cagccgcctt cagcgaacga tcggcaacac
     5101 cgcctgcggg aagtgatgtt ttatctcaac ttgtcggagc aggaaaaaga cgcattttgt
     5161 gaacgcttcg gcgttcgctt gctgacgtct tatgggatga cggaaaccat tgtgggcatt
     5221 atcggcgatc gccctggcga taaacgacgc tggccgtcga ttggtcgggc ggggttttgc
     5281 tacgaagcgg agatccgcga cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa
     5341 atctgtatta aaggcgtacc agggaaaacc atcttcaaag agtattttct caacccgaaa
     5401 gccactgcaa aagtgctgga agctgatggc tggctgcata ccggcgatac cggataccgc
     5461 gacgaagaag gcttttttta tttcgtcgat cgccgctgca atatgatcaa acgcggtggc
     5521 gagaatgtct cctgcgtgga gctggaaaat atcatcgcca cccatccaaa aattcaggat
     5581 atcgtggttg tgggtattaa agattcgatt cgcgatgaag ccatcaaagc atttgtggtg
     5641 ctgaatgaag gtgaaacatt gagcgaagag gaatttttcc gcttctgcga acaaaatatg
     5701 gcgaaattta aagtgccctc ttatctggag atcagaaaag atctgccacg taattgctcg
     5761 gggaaaataa ttagaaagaa tttgaaataa atgaaacgtc agggaactac cctgccagct
     5821 aataatcacg ctctaaaaca atatgcattt tttgcaggaa tgttgtcgtc cctgaaaaag
     5881 caaaaatgga gaaaaggaat gagtgaatca ttacatctga cccgcaatgg atcaattctg
     5941 gaaattaccc ttgatcgccc aaaagcgaat gctattgatg caaaaaccag ctttgaaatg
     6001 ggcgaagtat ttctaaattt ccgtgacgat ccgcaattac gtgtcgccat tattaccggt
     6061 gccggagaga aattcttttc cgcgggctgg gatttaaaag cggcagcaga aggcgaagca
     6121 ccggatgctg actttggccc agggggtttt gcgggattaa ccgaaatttt caatctcgac
     6181 aaaccggtta tcgcagctgt gaacggctac gcctttggcg gcggtttcga actggcgctg
     6241 gcggcagatt ttattgtttg tgccgataac gccagcttcg ccctgccgga agccaaactg
     6301 ggcatcgttc ctgacagcgg cggtgtgctg cgtctgccga agatcctgcc gcctgccatc
     6361 gtcaatgaaa tggtgatgac cggcagacga atgggcgcag aagaggcgct gcattggggg
     6421 atagtcaacc gcgtggttag ccaggcggaa ctgatggata acgcccgcga actggctcag
     6481 cagctggtta acagcgcccc gctggcgatt gcggcgctga aagagatcta ccgcaccacc
     6541 agcgaaatgc cggtagaaga agcgtatcgc tatattcgca gcggcgtgtt gaaacactat
     6601 ccatcggttc tgcattcgga agatgccatt gaagggccgc tggcgtttgc cgagaagcgc
     6661 gatccggtgt ggaaaggacg ttaacaaccg tgagctatta cgcctttgag gggttaattc
     6721 cggtggttca cccgacggcg tttgtccatc ccagtgcagt cttgattggc gatgtgattg
     6781 tgggagccgg tgtctacatt ggcccactcg cctcactgcg tggtgactac gggcggttga
     6841 tcgtgcaaac gggagccaat attcaggatg gctgcattat gcatggctac tgcgacactg
     6901 acactatcgt tggggaaaac ggccatatcg ggcacggagc gatcctgcat ggttgtgtga
     6961 ttggtcacga tgcattggtc gggatgaaca gcgtgattat ggatggtgcg gtcattggcg
     7021 aagagagcat tgttgccgcc atgagctttg tcaaagcggg ctttcgcggc gagaaacgtc
     7081 agttgttgat gggtacgcca gcccgcgcgg tacgcactgt tagtgacgac gagttacact
     7141 ggaaacggtt gaataccaaa gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac
     7201 atgaaacaca gccactgagg caaatggagg aaaatcgccc ccgtttgcag gggacgacgg
     7261 atgtgacgcc taaacggtga tcttgccgga tgcggcgtaa acgccttatc caactctgct
     7321 ggagagcaac cgtaggccgg ataagatgcg ccagcatcgc atccggcgac caggtcgcac
     7381 ggtatcatcg aaatgacatt aacgacgcat actctttgac aacatcatct gccacttctg
     7441 ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac
     7501 taatttttcc cgactgttat tgcgcaggcg cgcataaatt tgctcatcga tatcgaccac
     7561 tttaaccagt cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac
     7621 ttcgcagctt atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct
     7681 gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat
     7741 catccattcg gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac
     7801 tccagtttca caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaaggcca
     7861 tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa
     7921 tcctgcttat gtcatcaaaa atatcgattc cccaaaagat ttacttaatg ggcaaaaaaa
     7981 acggtcctca tcagagaacc gtattagtta ttcgatcaaa ttagcggatg aaaaatatcc
     8041 gccatgacac actattattt gatctgcgcg tgcatttcct gcaccgaaat tactttttca
     8101 gtcgcatcgg cattcagcgc catcgcggta gcgaaaccac cgttcagggt ggtgtcataa
     8161 tgcactttat attgcagcgc actgcgacga atcacgcggg agtcttcaat cgcgcgacga
     8221 cctgaggtgg tgttgatgat gtaggtatat tcgccattct taatacggtc ctgaatgtgc
     8281 ggacggcctt catgcacctt gtttaccaga cgcggattga tacccgcttc gcccagcaca
     8341 atcgccgtgc cgtgggtcgc atccagctcg aagccctgtt tcagcagttt tgccgccagg
     8401 tccaccacgc gttctttatc gccttcgcgc acggaaagca gcgcacgacc gtgtttcttc
     8461 atggtggagt tgctgcccag ctgcgctttg gcaaacgctt cagcgaaggt gcggcccacg
     8521 cccatgactt ccccggtaga gcgcatttct ggccctaaca gcgggtcaac gcccgggaat
     8581 ttattgaacg gcagcaccac ttctttcacc gagtagtacg gcgggataac ttctttggtt
     8641 acgccctgct cagccagcga tttgccagcc atcacacgcg ccgccacttt tgccagcggt
     8701 acgccggtgg ctttggagac gaacggaacg gtacgcgccg cacgcgggtt aacttcaatc
     8761 aggtagactt cgttgttttt caccgcaaac tgcacgttca tcaggccgcg cacctgcaat
     8821 tcgaaggcca gtttctgcac ctgctggcgc atcacatcct gaatttcctg acttaaggtg
     8881 taggctggca gagaacatgc ggagtcaccg gagtgcacgc ccgcctgctc gatgtgctcc
     8941 ataatgccgc caatcagcac catttcgccg tcgcagatgg catccacgtc aacttctact
     9001 gcgtcatcaa ggaaatgatc cagcaacact ggcgcatcgt tagacacgct aaccgccgtc
     9061 tggaagtagc gacgcaggtc agcttcgtca tagacgattt ccatcgcccg accgccgaga
     9121 acgtaagacg gacgcaccac cagcgggtag ccaatctctt ttgccttctc aactgccatt
     9181 tcaatagcag taacggtggc gttcgccggt tgtttcagtt tcagacggtc aaccgcatgc
     9241 tggaagcgtt cacggtcttc cgcacggtcg atagcatccg ggctggtgcc gataaccggt
     9301 acgccagcgg cttccagtgc acgcgccagt ttcagcgggg tctgaccgcc gtactgaacg
     9361 ataacgcctt tcggcttctc aatacgcacg atttccagca catcttccag agttaccggt
     9421 tcgaagtaga ggcgatcgga agtgtcgtag tcggtagaga cggtttccgg gttacagtta
     9481 accataatgg tttcgtaacc gtcttcacgc agcgccagcg aggcgtgtac gcagcagtag
     9541 tcgaattcga taccctgacc gatacggttt gggccgccgc caagcaccat gattttttca
     9601 cggtcggtag acggattcgc ttcgcactct tcttcataag tggagtacat gtaagcggtg
     9661 tcagtggcaa actctgccgc acaggtatcc acgcgcttgt agaccgggtg caggtcatat
     9721 tggtcacgca gcttacggat ttccgcttcg cgtacgcccg ccagttttgc caagcgcgca
     9781 tcggcaaagc ctttgcgttt cagctggcgc aggaattcag cgttcaggcc agtgatgccc
     9841 acttccgcca ctttctcttc cagacgcacc agttcttcaa tctgtaccag gaaccagcgg
     9901 tcaatgttag tcaggttgaa gacgccgtcc acagacaggc ccgcgcggaa agcatcggcg
     9961 atgtaccaga tacgctctgc gcctgcgtct ttaagttcgc gacggatttt ggttaacgct
    10021 tccgggtcat ccaggctcac tttcgggtcg aatccagtcg caccgacttc caggccgcgc
    10081 agcgcttttt gcagggattc ctgctgcgtg cgaccaatcg ccatcacttc gccaaccgat
    10141 ttcatctgag tggtcagacg gtcgttagca ccggcaaatt tttcgaagtt gaagcgagga
    10201 attttagtaa ccacatagtc gatggacggc tcgaaggagg ccggagtacg tccgccagtg
    10261 atgtcgttca tcagttcgtc gagggtgtaa cccaccgcca gtttcgccgc cactttagca
    10321 atcgggaaac cggtcgcttt cgacgccagc gccgaagaac gggacacgcg tgggttcatt
    10381 tcgataacaa tcaggcgacc gtttttcggg ttcaccgcaa actggacgtt ggaacccccg
    10441 gtttcaacgc cgatttcacg cagcaccgcc atcgaggcgt tacgcatgat ttgatattct
    10501 ttgtcggtca gcgtttgggc tggcgcgaca gtgatggagt caccggtgtg gatgcccatc
    10561 gcatcaaagt tttcgataga gcagacgatg atgcagttgt cgtttttatc acgcaccact
    10621 tccatctcgt actctttcca gccgatcagc gactcatcaa tcagcaactc tttggtcgga
    10681 gagagatcca gaccacgggc gcaaatttct tcaaactctt cacggttata agcgataccg
    10741 ccgccgctac cgcccatggt aaaggatggg cgaataatgc acgggaagcc cacgtcagcg
    10801 gcaaccgcca gcgcttcttc catcgtgtgt gcgataccgg aacgcgcggt ttccagacca
    10861 attttcttca tcgctacgtc gaaacgacgg cggtcttctg ctttatcaat cgcatcggca
    10921 gtggcaccaa tcatggtgac gccgaactct tccaacacgc cctgacgttc cagctccagc
    10981 gcgcagttca gcgccgtctg accgcccatc gttggcagca ccgcgtccgg gcgctctttt
    11041 tcaataatct tgcgtaccac ttcccagtga atcggctcga tgtaggtcgc atcggccatt
    11101 tccgggtcgg tcatgatggt cgccgggttg gagttcacca gaatgacgcg gtaaccctct
    11161 tcgcgcaggg ctttacatgc ttgcgcgcca gagtagtcaa actcacacgc ctgaccgata
    11221 acaatcgggc ccgcacccag aatcaggata ctttttatat ctgtacgttt tggcatggct
    11281 cttttactcc tgattactta gcggttttac ggtactgctc aattaactcg ataaagtggt
    11341 cgaacaacgg cgcggcgtcg tgtggaccag ggctggcttc agggtgaccc tggaagctga
    11401 acgccggttt atcggtgcga tgaatgccct gtaacgtacc gtcgaacagg gatttatgcg
    11461 tgacacgcag gtttgcaggt aatgttgctt cgtccaccgc aaaaccgtgg ttctgggcgg
    11521 tgatcatcac cacgtttttc tccacatctt taaccggatg gttgccgccg tggtgaccaa
    11581 atttcatttt gacagtcttc gcaccgctcg ccagcgccag cagctgatga ccgaggcaga
    11641 tgccgaatac cggaatatcg gtttcgagga atttctggat ggcggtaatg gcgtaatcgc
    11701 acggggccgg gtcgccagga ccgttggaga ggaagatgcc gtctggattc attttcagca
    11761 catcttccgc agaagtttgc gccggaacga tggtcaggcg acagcctcta tccaccagca
    11821 tccgcaggat gttgcgcttg gcaccaaaat cataagccac gacgtggaac ggcagctcgt
    11881 cttctttttt cgcttctggc aggccgccgg tcaacgtcca gctcccttgt gtccagctat
    11941 aggcttctgc ggtggtcact tcttttgcca gatccatgcc attcagacct gggaacgcgc
    12001 gggctttttc taacgccagc gccgcatccg ggttatcgcc tgcgataatg cagccattct
    12061 gtgcgccttt ctcgcgcagt aaacgcgtca gcttacgggt atcgatatcg gcaatcgcca
    12121 cgatgttatg gcgcttcagg taagaagaga ggtcttcggt attacggaag ttgctggcaa
    12181 tcagcggcag atcgcgaatc accagacctt gtgcatgtac ctgagaagat tcttcatcgg
    12241 cgtcattagt gccgacattg ccaatatggg gataagtaag agtaacgatt tgacgagaat
    12301 aggaaggatc agtgaggatt tcttgataac cggtcattga agtattgaaa acgacttccc
    12361 caaccgccga acctgttgcc cctatggccc gaccgtgaaa ctgggttccg tcttccagaa
    12421 ccaatagcgc tgacttaatc aaaacaccct ccagagaata ttcactcact ttatttgcat
    12481 attaattcac aatgatgacg taaatcaatg caaatctgct taccgacgaa tttctggcaa
    12541 acggcggcat tctggagata tgggcgctaa aagtcaactt aatggcagga ttttttatat
    12601 tattttgtgg atcatgcatg ttttacaggc attaagcgac aaaaagatca tctaacctgt
    12661 tctggaaaac gcttgcgcag caagaagatt gcatgaaata aaaacgagca ggaaaaagtg
    12721 gaccaaatgg tcaaaattta cattaaaaca acaaaaatag aaggtaaaac atgtgatttt
    12781 tagcgtttgc ataaaattta aaaacataac acaaaaaata aaagggctaa aaattaaagc
    12841 cctttatatt caataaatta aatgcgttac ttttgcatca ccataaacac tttgtggtta
    12901 caaattatgg agatcaagca catctcgcat atcaaaaaga ccactttcct tgccactcaa
    12961 ccacaaagcc gatcttaccg caccgttagc aaatgtcatg cggctggacg ccttatgggt
    13021 gatctccaga cgctcgccaa tatcggcaaa catcgcggta tgttcgccaa cgatgtcacc
    13081 tgcacgcacg gtggcaaaac caatggtacc aggcacacgt tcaccggtat ggccttcacg
    13141 actgtagacc gcgcaatctt tcagatcttt atcaagggcg tgggcgatcg cctctcccat
    13201 tgccagtgcg gtgcctgacg gcgcatcaac tttatgtcta tgatgtgctt caataatttc
    13261 gatatcggtg tagtcaccca tcactttggc tgctttctcc agcagcttaa gcatgacgtt
    13321 aacgccaacg ctaaaattgg cggcaaagac aatcgcaata tcggcggcgg cgtcacgaat
    13381 cgcctgttta ccggcttcgt caaaccccgt agtgccgatc accatccctt tgccatgctg
    13441 gcgacaaaaa gcgagatggt tcagcgtacc ttccggacgg gtaaaatcga taaacacatc
    13501 aaaatcatct tttatcgcat caaggctgct ttgcacggta acgcctgttt tccctgcacc
    13561 agccagctca ccggcgtcgc tgcccagtaa agaagatcct tcgcgctcca gcgccgcgcc
    13621 cagctgcacg ccttctaatg ccagcgccgc ctgaatcaac tggcggccca tacgcccccc
    13681 ggctcccgcg atggcaacgc ggatgtttgc atcatgcata gctattctct tatgttaatt
    13741 tgcatagacc gttttcagag taaccagcgc atcgctacgc cgccagccaa aaacgctgat
    13801 aattagaaat taatgataaa cagggaggga tatcagtgaa aggcatgagt cgatgaatga
    13861 ctgcatgccg ataacatgtg acaggttacg gcaccagagc cagcacctca gccacccact
    13921 gttggaagcc ttttacattc agatccaatg ccacctgtac attggctggt ttgcccaggc
    13981 aaccgtcgat atcaaccacc gtcgtgcctg aggtaaattc gccttgagtt tctaccgcga
    14041 caaaacacgg cttgagggtg aataagtccg ggcgcaccag ccaggcgatg gcgcagagat
    14101 cgtgcattcg cagaccgctt tgcatactgc cgctacggta gtggctaaac agggcgtgga
    14161 gcatttttcc ggtgcgattc aactccggca aagtagcgag atagtcaggg gttaatattg
    14221 cctgattggt gacatccaga ccgcacatga cgatttcgat gccgctacgg aagacacagg
    14281 ctgccgcttc tggatcggca gcgatattaa actcggcgtt tggtgtacag ttgccgcgac
    14341 cagcagaacc gcccatgatc accagacgac gaatatacgg tttacattcc gggcattgtg
    14401 aaagtaacag cgcaatattg gttaacgggc cgatggccac cagggtaacg ggctctggtg
    14461 cacgcatcag ggcatcccga atcgccagaa acgccggtat cccgagtggc tttcggttgt
    14521 gctcaacaaa gtcgtagtca gccattcccg attcgccgtg cacagatgct gcatcacgcg
    14581 gtgcgcgtac cagcggcaca gcggctccct gggcgagcgg aatctccgca ttccagaaat
    14641 gcagcagttg cagggcgttg cgggtggttt tctcaaccga gacattaccc gcgacggtgg
    14701 tcatcagttg caggtcgagt tcgggggcaa aaatcgcggc ggcaatggcg acggcatcgt
    14761 caatgccggg gtcagtatcg aggaagatag gtaaacgcat gttttctcca taaaaaatgc
    14821 cgataacaag accggcattt tcgcataact taggctgcta atgacttaat cgacttcacg
    14881 aatatcgaca cgcagctctt tcggcacttc gaaaacaata ttttcttcac ggccttccag
    14941 cggaatggct tcaccaccac ccagctgctg caaacgtgcc accacattct gcaccagaat
    15001 atccggagcc gatgcgcccg cagtcacgcc gacgcattta acctctttca cccactcttc
    15061 ctggatatct ttcgcatcgt caatcaaaaa cgcgcgtttg cccatacgct gggccagctc
    15121 cgccagacgg ttggagttgg aggagttttt cgaaccgacc accagcacaa cttccgcctg
    15181 ttctgccaag gcacgtaccg cttcctgtcg gttagtcgtg gcgtagcaga tgtcatcttt
    15241 gcgcggaccg acaattttcg ggaagcgttt acgcagcgcg tcgatcacat cagacgtgtc
    15301 atccaccgac agcgtggtct gggtcataaa ggagagtttc tcttcgtttt tgaccgtcag
    15361 tttccacaca tcgtccggcg attcgaccag atacattccc ccttccgggt tgctgtactg
    15421 acccattgtc ccttccactt ccgggtgccc ggcgtgaccg atgagaatag attcttcgcc
    15481 acgacggctg gcgcgggcga cttccatatg cactttggtc accagcggac aggtggcatc
    15541 aaacaccgtc aaatcgcggc tttttgcttc gttacgtacc gcctgagaaa caccgtgtgc
    15601 ggagaaaatc aggatcgcgc cgtccggtac ttcgctaatc tgctcaataa agatagcccc
    15661 acgctcacgc aagctatcga ccacatagcg gttatgtacc acttcgtgac ggacatatat
    15721 cggtgcgccg taaatggcca gcgcgttttc aacaatgctg atagcgcggt ctaccccggc
    15781 acaaaaacca cgcgggttgg ccaacaggat ctgcatgtta cgcctccagt gccggatcga
    15841 tttccagcac ttcaatatca aaatgaacgg tctgcccggc cagcggatgg ttgaaatcaa
    15901 cggtaatgga gtcgccgtta atttcgcgga tcacgccagg catctcactg ccatccattg
    15961 cggtaaaaag cataattgcg ccaatttctg gctcgcctgc atccataaat tcacggcggg
    16021 agaagtactg aatcaggtcc ggtgacggca cgccaaacgc cgcatctggc tccagcgaga
    16081 aggtggtttt atcgcccact ttcagcccca gcaggtgttg ctccagccct tcagaaagag
    16141 aagcatcgcc caggcggaac agcgccggtt taccgttgtt gcgggtagac tcggcggtgg
    16201 tgccatcgtc gagttttagc gtgaagtgca ccaggacggc gctattgctc tgtacagatt
    16261 cagacatgca ggtttgctct tatattttga ggatttttgc cggatgcggc ctacgatttc
    16321 gcagcaatct gtaggcctta tccggcaggg tttattattg ttttttcgct ttagaaggca
    16381 aaaaaccttc cagcacaatc agtgccgcac cgacacaaat ggcagtatcg gcaaggttga
    16441 aggtggcgaa gtgccagtcg ccgacgtaga agtcgatcat atcgacaacg aagccgtgcc
    16501 acaggcggtc gaacaggttg cccagcgcgc cgccaataat cagcgcgtaa gcgatattgt
    16561 ttagcttctg cgtggccttc gagcgataca tcatcactgc caggatcacg ctaataccaa
    16621 tcgcaatacc ggcaaagaac caacgctgcc agccgccgct atcggcaagg aaactaaacg
    16681 ccgcgccata gttacgcgca taatgcagat taagcgacgg gaacagcggg accgtatccc
    16741 ccagagcaaa gttctggagg atcaggtatt tgctgcccag atcgataatc agcacgacta
    16801 ccaccagcca cagccagcgt agccctgttg aacagatcga ttgactcatc aggcaaactt
    16861 acgtttttca ccgtcaccgg cgacgttgct gacacagcgg ccgcagattt ctgcgtgttc
    16921 cgccaccttg ccgacatcct gggtgtagtg ccagcagcgt gggcacttct caccttcggc
    16981 tttactcaac gcgactttca gccctttgag tacttcgctc tgctgagcat cagcaggtgc
    17041 gtcgttatag tctgcaacgg tagcgccgga ggtcaacagg acaaatcgta attcatcgcc
    17101 cagcgcggtc agtttcgccg ccagttccgg ttctgcatac aaggttactg ccgcttccag
    17161 cgagccaccc actttcttgt cggcacgcgc ttgctcaatg actttgttca cttcgccacg
    17221 cactttcaac agctcgtccc agaacgcatc gttcattgct tcactgtctg ccagaccaaa
    17281 caggccttcg taccactcac cggtgaagac gtatttttca cgttcgcccg gcagatagcc
    17341 ccacacttca tcagcggtga aggagaggat tggtgccatc cagcgcacca gcgcttcagc
    17401 gatgtgatac agcgcagtct ggcagctacg acgcgccacg ctatccgctt tggcggtgta
    17461 ctgacggtct ttgatgatgt cgaggtagaa ggaacccatc tcaacggagc agaagcgcat
    17521 cagacgctgt accacttcgt ggaaatcgta tgcttcgtac gccttgagga tgtcttcctg
    17581 tgccgctttc gtgcaaccta cggcccagcg atccagtacc accatctctt ccggtttcac
    17641 catatctttt gctggatcaa aaccattcag gtttgccagc aggaagcgcg cggtgttacg
    17701 gatacgacga tagctgtcgg cagcacgttt caggatctcg tcagaaacgg ccatttcacc
    17761 ggtgtagtca gttgatgcca cccacagacg cagaatatcc gcacccagtt tgttcatcac
    17821 atcctgcggc gaaacggtgt tgccgatgga tttagacatc ttgcgaccct gaccatccac
    17881 ggtaaagccg tgggtcagta cctgacgata cggcgctttg cccttcatcg cggtggagat
    17941 cattagggaa gacatgaacc aaccacggtg ctggtcagaa ccttccagat acatgtccgc
    18001 tgcgtgacca gcaaattccg gacgcacgtc aacaacggaa gagtgggtag atccggagtc
    18061 aaaccataca tccagcgtat ccggcacttt cacatactgg tcagcttcgt cgccgaggag
    18121 ctctttcgca tcgagatccc accacgcctg gatgccgtca acttcaacgc gttttgccac
    18181 ttcttccatt agttcgaggg tacgcggatg cagctcttcc gtgtctttgt gcacgaacag
    18241 tgacatcggt acgccccagg tgcgctgacg ggagatacac cagtcaggac ggttagcaac
    18301 catcgactcg atacgcgcct ggccccagtc cgggatccac tgcacgcctt tgatctcttt
    18361 cagtgactgc gcacgcagac ctttctgatc catgctgacg aaccactgcg gtgtcgcgcg
    18421 gaagatgatc ggcgttttgt gacgccagca gcacggatag ctgtgctgca ttttctcaac
    18481 gtgcagcagc gcgccttttt cctgcagcag cgccacgacg atgtcgttcg ctttgaagac
    18541 gttcacgcca tccagcgtcg gataagtgcc cggcagataa gtgccgtccg ggccaaccgg
    18601 gttagcggtt tccaggccgt atttctgacc gatcacatag tcgtccgggc cgtggccagg
    18661 cgcggtgtga acggcaccgg taccggcatc cagggtaacg tgatcgccga ggattgccgg
    18721 aacgtcgaag cccataaacg gatgggtaaa gcgcagcagc tcaagctctg caccttttac
    18781 cgtgccgaga atggtgtaat cggtcacgcc gatacgctgc attacgcttt caaccagatc
    18841 tttcgccaga atcacggcct gaccgtcgat ctgcaccagc gcatagtcga aatcaggtgc
    18901 aatagagatt gcgcggttcg caggcagagt ccacggcgta gtggtccaga tcaccagcga
    18961 gattgggccg ttaacgttgc ttacgccaaa tttcgctttc agcgcatcct gatcgaccgc
    19021 ctggaaagcg acgtcaatgg acggagaagt tttgtcgtaa tactcaactt ccgcttctgc
    19081 cagtgcagaa cggcagtcta cgcaccagtg caccggcttc gcgcctttgt gcaggtgacc
    19141 attgccgatg attttgccca gcgcgcggat gatgttggct tcagttttga agtccatagt
    19201 cagatacggg tgcgaccagt cgcccagcac gcccagacgg ataaagtctt tgcgttgacc
    19261 gtcaacctgg gtcgccgcgt attcgcggca cttggcgcgg aactcggcgg cggtgaattt
    19321 ctcacccggc ttaccgtatt cttgctctac tttcagctcg atcggcagac cgtggcagtc
    19381 ccagccaggc acatacggcg agtcataacc ggaaagccct ttggacttca cgataatgtc
    19441 tttcagaatc ttgttaaccg agtgaccaat atgaatgctg ccattcgcat aaggagggcc
    19501 atcatgcaga atgaaggttt ttttgccttt tttagccgca cggatgatgc cgtacagatc
    19561 atcatcagtc caacgcgcca gcattcccgg ttcgcgcttg gcgagatcgc cacgcatcgg
    19621 gaaccctgtt tccggcaaat tcagggttga tttatagtca ctcatcagat tctcggttcc
    19681 gtgtttcggt ttgattacat aacaggctta agccggtttt gttagcccaa aaaattcgcg
    19741 ggcggttaat tcatcacgcg caatctgcgc tttcagttcg tccagcgacg caaatcgctg
    19801 ctcattgcgt atttttttac gcagcactac ttgtatatgg cgaccgtaaa ggtccattgc
    19861 aacatctaac aaatgcactt ccagctgctg gcgaataccg gcaaccgttg ggcgtgttcc
    19921 gatgtttgcc acgccgggta acggcttttc accgaggccc agcacttcta ccgcataaac
    19981 ccctttcacc ggggaaacct gacggcgtag cggtacattc gccgtcggga aacctatagt
    20041 gcgccctaat tcatcaccgt ggactacacg cccggagatg gcaaacgggt gccccagtaa
    20101 actttctgcc agagccagat tgtcatcagc cagcgcctga cgcacggcgg tgctgctgat
    20161 acgcacacca ccttcgcaaa aagtttgcgt actggtgata tcgaagccgt attccatgcc
    20221 agctttctgt aataacaaga aatcgccttc acgaccagcg ccaaagcgga aatcatcacc
    20281 tacggcaaga aattttacgc gcaaatgctt caccagaaga tcgctgatga aattttgcgc
    20341 ggttaacgcc gcgaaacgcc tgtcgaaacg tacgcacagc acgtaatcaa cgccacactc
    20401 tgcaaggtaa cgtagttttt cccgcagccg ggtcagccgt gccggggctt tatcggtagc
    20461 aaacagttcc agtggttgag gttcaaaaag catcaccatc accggtaagt tgcgcttgcg
    20521 cccttcttcc tgcaagccct gtaacagcgc gcgatgaccg cgatgcacgc cgtcgaaatt
    20581 accaatagtc agcacacacc cttcttgcgg ggcctggctg agattatgta tgccgcgtat
    20641 cagcttcatg tctggctcaa aacagtgaaa atcgtccgag tataccttgt acagcggtca
    20701 aggttaaccg gcgattgagt accgaaatca gaaagaagtg gcttttcatt gtcatggcgc
    20761 aaatcacggg aagaaactga ctgcctgctg caatttttat cgcggaaaag ctgtattcac
    20821 accacgcaag ctggtagaat cctgcgccat cactacgtaa cgagtgccgg cacattaacg
    20881 gcgcttattt gcacaaatcc attgacaaaa gaaggctaaa agggcatatt cctcggcctt
    20941 tgaattgtcc atatagaaca catttgggag ttggaccttg gctaatatca aatcagctaa
    21001 gaagcgcgcc attcagtctg aaaaggctcg taagcacaac gcaagccgtc gctctatgat
    21061 gcgtactttc atcaagaaag tatacgcagc tatcgaagct ggcgacaaag ctgctgcaca
    21121 gaaagcattt aacgaaatgc aaccgatcgt ggaccgtcag gctgctaaag gtctgatcca
    21181 caaaaacaaa gctgcacgtc ataaggctaa cctgactgca cagatcaaca aactggctta
    21241 atcgccaatt tgctgaagct ttgtgaaaaa gcccgcgcaa gcgggttttt ttatgcctgc
    21301 tgcttttgtg atgcgtgaga gaacacactg ccggttgcca gatagtcaaa agagacatgc
    21361 cgttgtacta tctgaactct tcgtggctta agaatcgtag aaccatcggc tgatacattc
    21421 tgaacaagaa tatctactca atctccagac tcccccacaa agaatatgga tattgtgata
    21481 cacat
//
LOCUS       DAPGRN010000083        21191 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPGRN010000083 DAPGRN010000000
VERSION     DAPGRN010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.083"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(327..641)
                     /gene="fliE"
                     /locus_tag="RHW33_003222"
     CDS             complement(327..641)
                     /gene="fliE"
                     /locus_tag="RHW33_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV1646700.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            856..2514
                     /gene="fliF"
                     /locus_tag="RHW33_003223"
     CDS             856..2514
                     /gene="fliF"
                     /locus_tag="RHW33_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV1646701.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKILPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LVAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2507..3502
                     /gene="fliG"
                     /locus_tag="RHW33_003224"
     CDS             2507..3502
                     /gene="fliG"
                     /locus_tag="RHW33_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV1646702.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3495..4181
                     /gene="fliH"
                     /locus_tag="RHW33_003225"
     CDS             3495..4181
                     /gene="fliH"
                     /locus_tag="RHW33_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV1646703.1"
                     /translation="MSDNLPWKTWTPVDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4181..5554
                     /gene="fliI"
                     /locus_tag="RHW33_003226"
     CDS             4181..5554
                     /gene="fliI"
                     /locus_tag="RHW33_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV1646704.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            5573..6016
                     /gene="fliJ"
                     /locus_tag="RHW33_003227"
     CDS             5573..6016
                     /gene="fliJ"
                     /locus_tag="RHW33_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV1646705.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6013..7140
                     /gene="fliK"
                     /locus_tag="RHW33_003228"
     CDS             6013..7140
                     /gene="fliK"
                     /locus_tag="RHW33_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV1646706.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPEDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAANPLIIPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFNGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7245..7709
                     /gene="fliL"
                     /locus_tag="RHW33_003229"
     CDS             7245..7709
                     /gene="fliL"
                     /locus_tag="RHW33_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV1646707.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7714..8718
                     /gene="fliM"
                     /locus_tag="RHW33_003230"
     CDS             7714..8718
                     /gene="fliM"
                     /locus_tag="RHW33_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV1646708.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLNPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8715..9128
                     /gene="fliN"
                     /locus_tag="RHW33_003231"
     CDS             8715..9128
                     /gene="fliN"
                     /locus_tag="RHW33_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV1646709.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9131..9496
                     /gene="fliO"
                     /locus_tag="RHW33_003232"
     CDS             9131..9496
                     /gene="fliO"
                     /locus_tag="RHW33_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV1646710.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            9496..10233
                     /gene="fliP"
                     /locus_tag="RHW33_003233"
     CDS             9496..10233
                     /gene="fliP"
                     /locus_tag="RHW33_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV1646711.1"
                     /translation="MRRLLSVAPVLLWLTTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10243..10512
                     /gene="fliQ"
                     /locus_tag="RHW33_003234"
     CDS             10243..10512
                     /gene="fliQ"
                     /locus_tag="RHW33_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV1646712.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            10520..11305
                     /gene="fliR"
                     /locus_tag="RHW33_003235"
     CDS             10520..11305
                     /gene="fliR"
                     /locus_tag="RHW33_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV1646713.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANNVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLMVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            11595..12218
                     /gene="rcsA"
                     /locus_tag="RHW33_003236"
     CDS             11595..12218
                     /gene="rcsA"
                     /locus_tag="RHW33_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV1646714.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12262..12450)
                     /gene="dsrB"
                     /locus_tag="RHW33_003237"
     CDS             complement(12262..12450)
                     /gene="dsrB"
                     /locus_tag="RHW33_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV1646715.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            12613..12840
                     /gene="yodD"
                     /locus_tag="RHW33_003238"
     CDS             12613..12840
                     /gene="yodD"
                     /locus_tag="RHW33_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV1646716.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13139..13954
                     /locus_tag="RHW33_003239"
     CDS             13139..13954
                     /locus_tag="RHW33_003239"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV1646717.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(13951..15644)
                     /gene="yedQ"
                     /locus_tag="RHW33_003240"
                     /pseudo
     CDS             complement(13951..15644)
                     /gene="yedQ"
                     /locus_tag="RHW33_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(15815..15997)
                     /locus_tag="RHW33_003241"
     CDS             complement(15815..15997)
                     /locus_tag="RHW33_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV1646718.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(16076..16993)
                     /locus_tag="RHW33_003242"
     CDS             complement(16076..16993)
                     /locus_tag="RHW33_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV1646719.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17166..18086
                     /gene="yedA"
                     /locus_tag="RHW33_003243"
     CDS             17166..18086
                     /gene="yedA"
                     /locus_tag="RHW33_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV1646720.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGIFLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18075..18545)
                     /gene="vsr"
                     /locus_tag="RHW33_003244"
     CDS             complement(18075..18545)
                     /gene="vsr"
                     /locus_tag="RHW33_003244"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV1646721.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(18529..19944)
                     /gene="dcm"
                     /locus_tag="RHW33_003245"
     CDS             complement(18529..19944)
                     /gene="dcm"
                     /locus_tag="RHW33_003245"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV1646722.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHHARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRS"
     gene            complement(20011..20706)
                     /locus_tag="RHW33_003246"
     CDS             complement(20011..20706)
                     /locus_tag="RHW33_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV1646723.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPVG"
     gene            complement(20746..21111)
                     /locus_tag="RHW33_003247"
     CDS             complement(20746..21111)
                     /locus_tag="RHW33_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1646724.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVISRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5137 a         5639 c         5447 g         4968 t
ORIGIN      
        1 atattgccca gggcgcattc taaaacacaa taaaattacc actatcaaaa atcgctcata
       61 tccatagccc ggaatatgaa taaaaacatc aacaaagatt aaattatcca gaggttactg
      121 ttgcgtctgt ggtcgtaaca acatccgacg tggtgatgtg tcaaatgaga cactctgatg
      181 atcagagggt ttatcgcgta tacggatcgt ggatggcaga aaacgttcag gctcaggtat
      241 ccattttaaa ccagaaaatg agtgagtttg ccccatccat gccccacgcg gtgagatcgg
      301 atgtaatcaa caacaggtta caaaacctac acctgcatgc tcatcacttc ctgatacgcc
      361 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
      421 tcggtcatca catcgtttaa cgccacgccg ggttcaccga gagtgaattt ttctgcctgc
      481 gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca
      541 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
      601 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcatctc ctggataatt
      661 tctggtagca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
      721 acgggtttat tggcggatag aaaaaaacga aagcacaaat aatgggagcg tcaatttttc
      781 gagtttgctg acccgggagt gagtcttgtt ccactttgcc aataacgccg tccataatca
      841 gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa aatctcttga gtggcttaat
      901 cgcctgcgtg cgaatccgaa aattccattg attgttgccg gttccgcggc agtggcggtc
      961 atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc gcacattatt cagcaatctt
     1021 tccgatcagg atggtggcgc aattgtcagc caactgacgc aaatgaatat tccttaccgc
     1081 ttcagcgaag ccagcggcgc tattgaagtt ccggcagata aagttcacga gctgcgcctg
     1141 cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact actcgatcag
     1201 gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc
     1261 gagctttctc gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca
     1321 atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta
     1381 aatctgttac ccggccgcgc actcgatgaa gggcaaatta gcgccattgt gcatctggtt
     1441 tccagcgccg ttgctggtct gccgccggga aacgtcacgc tggtggatca gggcggacat
     1501 ctgttaaccc agtccaatac cagcgggcgc gatcttaatg acgctcaatt gaaatatgcc
     1561 agcgatgtcg aaggccgtat tcagcggcgt attgaagcaa tcctgtcgcc tattgttggt
     1621 aacggtaata ttcacgccca ggtcacggcg caactggact tcgccagtaa agaacaaacg
     1681 gaagaacagt atcgccctaa cggtgatgaa tctcaggcgg cgcttcgttc acgccagctt
     1741 aatgagagcg agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat
     1801 caaccggcac ctgcgaataa cgcgccaatc agcacgcctc cggcaaatca aaataaccgc
     1861 cagcagcagg cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc
     1921 agtaactacg aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa
     1981 cgtctgtcag tcgcggttgt ggtgaattac aaaatcttgc cagatggcaa accgttgcct
     2041 ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc gcgaggcgat gggcttttct
     2101 gaaaaacgcg gtgattcgct caatgtcgtt aactcgccgt tcaatagcag tgacgaaagc
     2161 ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg atcagctagt ggctgccggt
     2221 cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc gtaaagcggt acgcccgcag
     2281 ctaacacgtc gcgctgaggc gatgaaagct gtacagcaac aggcgcaggc ccgcgaggaa
     2341 gtggaagatg cggtggaagt ccgcctgagc aaagacgaac aactacaaca acggcgcgct
     2401 aaccaacgac tgggtgcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat
     2461 ccgcgcgtgg tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac
     2521 aggcaccgat aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt
     2581 gttcaagcac ctctcccagc gcgaggtgca aaccctgagc gctgcaatgg cgaacgtcac
     2641 gcagatctcc aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca
     2701 gtttgccgca ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct
     2761 gggtgaagaa cgtgccgcca gcctgctgga agatattctc gaaactcgcg ataccgccag
     2821 cggtattgaa acgctcaact ttatggagcc acagagcgcc gccgatctga ttcgcgatga
     2881 gcatccgcaa attatcgcca ccattctggt gcatctgaag cgcgcccagg ccgccgatat
     2941 tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg
     3001 cggcgtgcag ccagccgcgt tggcggaact gaccgaagta ctgaatggct tgctcgacgg
     3061 tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct
     3121 gatgaaaact cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct
     3181 ggcgcagaaa atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgacgatcg
     3241 cagcattcag cgtctgttgc aggaagtgga ttccgaatcg ctgttgatcg cgctgaaagg
     3301 ggccgagcag ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat
     3361 tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg tcgcaggtgg aaaacgaaca
     3421 gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact ggcgagatgg taattggcag
     3481 cggcgaggat acctatgtct gataatctgc cgtggaaaac ctggacgccg gtcgatctcg
     3541 cgccaccaca ggcagagttt gtgcccatgg tcgagccgga agaaaccatc attgaagagg
     3601 ctgaacccag ccttgagcag caactggcgc aactgcaaat gcaggcccat gagcaaggtt
     3661 atcaggcggg tattgccgaa ggtcgccagc aaggtcataa gcagggctat caggaaggac
     3721 tggcccaggg gctggagcaa ggtctggcag aggcgaagtc tcaacaagcg ccaattcatg
     3781 cccggatgca gcaactggtc agcgaatttc aaactaccct tgatgcactt gatagtgtga
     3841 tagcgtcgcg cctgatgcag atggcgctag aggcggcacg tcaggtcatc ggtcagacgc
     3901 caacagtgga taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt
     3961 tattcagcgg taaaccacag ctgcgcgtgc acccggatga tctgcaacgt gtggatgata
     4021 tgctcggcgc taccttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccatc
     4081 ctggcggctg taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccacccgct
     4141 ggcaagaact ctgccgtctt gcagcaccag gagtggtata atgaccacgc gcctgactcg
     4201 ctggctaacc acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg
     4261 ctacgggcga ttaactcgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc
     4321 gctcggcgca acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag
     4381 cgaagtcgtt ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtcgaagg
     4441 tgtcctgccc ggcgcgcggg tttatgccaa aaacatttcg gctgaaggac tgcaaagcgg
     4501 caagcagttg ccgctcggtc cggcgttatt aggtcgcgtt ctggacggca gcggtaaacc
     4561 cctcgatggc ctgccctccc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt
     4621 taacccgttg caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa
     4681 tgccctgctt accgttgggc gtggccagcg tatggggctg tttgccggat ccggcgttgg
     4741 taaaagtgtg ctgctgggga tgatggcacg ttacacccgc gccgatgtca ttgtcgtggg
     4801 tttgattggt gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga
     4861 agggcgtgca cgctcagtgg tgattgccgc tccggcggat gtgtctccac tcctgcgaat
     4921 gcaaggtgcc gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt
     4981 gttgctgatt atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc
     5041 gattggcgaa ccacccgcca ccaaaggtta tccaccgtcg gtgtttgcca aattaccggc
     5101 actggtcgag cgtgccggaa atggcattag cggcggcggc tcgattaccg cgttttatac
     5161 cgtgctcact gaaggcgatg accagcagga tccgattgcc gactccgcgc gggcgatcct
     5221 cgacggtcac attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga
     5281 tattgaagcg tcgatcagcc gcgcaatgac ggcgttgatc agcgagcaac attacgcgcg
     5341 agtgcgcacc ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt
     5401 cggcgcgtat gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca
     5461 gctggagggc tatttacaac aaggcatttt tgaacgtgcg gactgggaag cgtctctcca
     5521 ggggctggag cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcaga
     5581 acatggcgcg ctggcgaccc tgaaagatct ggcagaaaaa gaggtagagg atgccgcgcg
     5641 cctgctgggt gaaatgcgtc gcgggtgtca gcaggcggaa gaacagctca aaatgctgat
     5701 cgattatcag aatgaatatc gcaataacct caacagcgat atgagtgccg ggatgaccag
     5761 caaccgctgg atcaactatc agcagtttat ccagacgctg gaaaaagcca ttactcagca
     5821 tcgccagcaa cttaatcagt ggacgcagaa agttgacatt gccctgaaca gctggcgaga
     5881 aaagaaacaa cgtttgcagg cctggcagac actgcaggaa cggcaatcca cggcggcact
     5941 gcttgcagaa aaccgcctcg atcagaaaaa gatggatgag ttcgcccagc gcgccgccat
     6001 gaggaaacct gaatgattcg cttagcgccc ttgattaccg ccgacgttga taccaccaca
     6061 ttgcctggcg gcaaagccag cgatgctgca caagattttc tcgcgttgtt gagcgaagca
     6121 ttagcaggcg agacaactac cgacaaagcg gccccccagt tgctggtggc aacagataag
     6181 cccacgacaa aaggcgagcc gctgatcagc gatattgttt ccgacgcgca acaagcggat
     6241 ttactgatcc ctgaggatga aacaccgcct gtcatcaacg acgaacaatc cacatcaaca
     6301 ccgttaacca ccgctcagac gatgacgttg gctgcggtgg ctggcaacaa tacggcaaaa
     6361 gacgaaaaag cggatgatct gaatgaagac gtcaccgcaa gcctgagcgc cctttttgcg
     6421 atgttgccgg gttttgacaa tacgcccaaa gtgactgatg cgccgtcaac cgtgttaccg
     6481 acagagaaac caacgctctt cacaaaactg acttctgcgc aactcacaac agcacagcct
     6541 gatgatgccc ccggcacacc agctcagcca ttaacaccgc tggtagcaga agcccagagt
     6601 aaagcggaag tcatcagcac accttcaccg gtgaccgctg ccgccaaccc gctaatcatt
     6661 ccacaccaga cacagccact gcccaccgtc gccgcgcctg ttttgagtgc accgctgggt
     6721 tctcacgaat ggcaacaatc attaagccag catatttcgc tgttcacccg ccaggggcaa
     6781 caaagtgcag agttgcgtct gcacccgcag gatttaggtg aagtgcaaat ctccctcaaa
     6841 gtggatgata accaggcgca aatccagatg gtttcaccgc atcagcatgt acgcgccgcc
     6901 ctggaagcag cgctgccggt actgcgtacg cagctggccg aaagtggcat tcagttaggg
     6961 caaagcaaca tcagtggcga aagctttaat ggtcagcagc aggccgcttc ccaacaacag
     7021 caaagccaac gcacagtaaa ccatgaacct ctggcggggg aagacgacga tacgcttccg
     7081 gttcccgtct ctttacaagg gcgtgtaaca ggcaacagcg gcgttgatat tttcgcctaa
     7141 cgtcagaggt agcaccgtaa tccgcgtctt ttccccgctt tgttgcgctc aagacgcagg
     7201 ataattagcc gataagcagt agcgacacag gaagaccgca acacatgact gattacgcga
     7261 taagcaagaa aagcaagcga tcgctttgga tcccaattct ggtattcatt accctcgcgg
     7321 cctgtgccag cgcaggttac agctactggc attcgcatca ggttgccgct gacgacaaag
     7381 cgcagcaacg cgtcgtgccc tccccggtct tctatgcgct ggataccttt acggtcaatc
     7441 tgggcgatgc ggatcgcgta ctttatatcg gcataaccct gcgactgaaa gatgaagcta
     7501 cccgctcgag gctgagtgag tatttgccgg aagtccgtag ccgtttgctg ttactgtttt
     7561 cgcgtcagga tgctgccgta ctggcgacag aagaaggcaa gaaaaacctg attgccgaga
     7621 ttaaaaccac gctttccacc ccgcttgttg ccgggcaacc gaaacaggat gtcaccgacg
     7681 tgctgtatac cgcttttatt ctgcgataac gacatgggcg atagtattct ttctcaagct
     7741 gaaattgatg cgctgttgaa tggtgacagc gaagtcaaag acgaaccgac agctagtgtt
     7801 agcggcgaaa gtgacattcg tccgtacgat ccgaataccc aacgacgggt tgtgcgcgaa
     7861 cgtttgcagg cgctggaaat cattaatgag cgctttgccc gccattttcg tatggggctg
     7921 ttcaacctgc tgcgtcgtag cccggatata accgtcgggg ccatccgcat tcagccgtac
     7981 catgaatttg cccgcaacct gccggtgccg accaacctga accttatcca tctgaaaccg
     8041 ctgcgcggca ctgggctggt ggtgttctca ccgagtctgg tgtttatcgc cgtggataac
     8101 ctgtttggcg gcgatggacg cttcccgacc aaagtggaag gtcgcgagtt tacccatacc
     8161 gaacagcgcg tcatcaaccg catgttgaaa ctggcgcttg aaggctatag cgacgcatgg
     8221 aaggcgatta atccgctgga agttgagtac gtgcgttcgg aaatgcaggt gaaatttacc
     8281 aatatcacca cctcgccgaa cgacattgtg gttaacacgc cgttccatgt ggagattggc
     8341 aacctgaccg gcgaatttaa tatctgcctg ccattcagca tgatcgagcc gctacgggaa
     8401 ttgttggtta acccgccgct ggaaaactcg cgtaatgaag atcagaactg gcgcgataac
     8461 ctggtgcgcc aggtgcagca ttcacagctg gagctggtcg ccaactttgc cgatatctcg
     8521 ctacgcctgt cgcagatttt aaaactgaac cccggcgacg tcctgccgat agaaaaaccc
     8581 gatcgcatca tcgcccatgt tgacggcgtc ccggtgctga ccagtcagta tggcaccctc
     8641 aacggtcagt atgcgttacg gatagaacat ttgattaacc cgattttaaa ttctctgaac
     8701 gaggaacagc ccaaatgagt gacatgaata atccggccga tgacaacaac ggcgcaatgg
     8761 acgatctgtg ggctgaagcg ttgagcgaac aaaaatcaac cagcagcaaa agcgctgccg
     8821 acgcggtgtt ccagcaattt ggcggtggtg atgtcagcgg aacgttgcag gatatcgacc
     8881 tgattatgga tattccggtc aagctgaccg tcgagctggg ccgcacacga atgaccatca
     8941 aagagctgtt gcgtctgacg caagggtccg tcgtggcgct ggacggtctg gcgggcgaac
     9001 cactggatat tctgatcaac ggttatttaa tcgcccaggg cgaagttgtc gtagtcgctg
     9061 ataaatatgg cgtgcggatc accgatatta ttactccgtc tgagcgaatg cgccgcctga
     9121 gccgttagtg atgaataacc acgctactgt gcaatcttcc gcgccggttt ctgctgcgcc
     9181 actgctgcag gtgagcggcg cactcatcgc cattattgcc ctgatcctcg ctgctgcctg
     9241 gctggtaaaa cggttgggat ttgcccctaa gcgcacaggc gttaacggtc tgaaaattag
     9301 cgccagtgct tcactgggcg cgcgtgaacg ggttgtggtg gtcgatgtgg aagatgcacg
     9361 gctggtgctc ggcgttaccg cagggcaaat caatctgctg cataaacttc ccccttctgc
     9421 accaacggaa gagataccgc agaccgattt tcagtcggtc atgaaaaatt tgcttaagcg
     9481 tagcgggaga tcctgatgcg tcgtttattg tctgtcgcac ctgtccttct ctggctgact
     9541 acgcccctcg ccttcgcgca actgccgggt atcaccagcc agccgctgcc tggcggtgga
     9601 caaagctggt cgctcccggt gcagacgctg gtgttcatca cctcgttgac gtttattccg
     9661 gcaattttac tgatgatgac cagtttcacc cgcatcatta ttgtttttgg tttattgcgt
     9721 aacgcgctgg gaacaccctc cgcgccacct aaccaggtat tgctggggct ggcactgttt
     9781 ttgacctttt ttattatgtc accggtgatc gacaaaattt atgtagatgc gtaccagcca
     9841 ttcagcgaag agaaaatatc aatgcaggag gcgctggaaa aaggggcgca gccgctgcgt
     9901 gagtttatgc tgcgtcagac ccgtgaggca gatttagggt tgtttgccag actggcgaat
     9961 accggcccgt tgcagggacc tgaagccgtg ccgatgcgca ttttgctccc ggcctacgtg
    10021 accagcgagt tgaaaaccgc atttcagata ggcttcacga ttttcatccc ttttttgatt
    10081 atcgacctgg tgatagccag cgtgttgatg gcattgggga tgatgatggt tcccccagcc
    10141 accattgctc tgccctttaa actgatgctg tttgtactgg tggatggctg gcaattgctg
    10201 gtcggttcgc tggcgcagag cttttacagc tagagaggca aaatgacacc tgaatcggtc
    10261 atgatgatgg ggactgaagc gatgaaagtc gcgctggcac tggctgcccc gctgttgctg
    10321 gtggcgctgg tcacgggcct tatcatcagt attttgcagg ccgccaccca gattaacgaa
    10381 atgacgctgt cgtttattcc aaaaatcatc gccgtattta ttgccattat tattgccgga
    10441 ccgtggatgc tcaatctgtt gctggattac gtccgcacct tgttcactaa cctgccgtat
    10501 atcatcgggt agccgtacta tgttgcaggt gacaagcgaa caatggctat cctggttaag
    10561 cctgtacttc tggccgttac tgcgcgtgct ggcgctgatc tccaccgcgc cgattctgag
    10621 cgaacgcagc gtaccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    10681 tgccccatca ttacctgcca acaatgttcc tgttttttcg ttctttgctc tgtggctggc
    10741 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    10801 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt
    10861 cgatccggcc agccatctta atatgcccgt tttagcgcgt atcatggata tgctggcgtt
    10921 actgctgttc ctgacattta acggtcattt atggttgatt tcactgatgg tcgatacctt
    10981 tcacaccctg ccgattggtg gcgaaccgtt gaacagcaat gcgtttctgg cactcaccaa
    11041 agcagggagt ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgct
    11101 gctgacactg aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt
    11161 tgttattgga tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc
    11221 gttaattgcg cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat
    11281 tattagtgaa ttgccattaa tataattccg taacgtttat catgttatcc taaggattat
    11341 ccgaaaaata atacctgcga acatcttcca ggatactcct gtggcgaaat atttgtttta
    11401 agctcactca catatcgcaa catttacttt actttaagac aattccaggc aaattataca
    11461 acactttacg ggatagtaag tccgcctgaa aaatcgcgag agtggcgcat taggtgaccc
    11521 atgttgttcc gtttagtcat gatgaaatat tcaggtaagg ggaattatcg ttacgcattg
    11581 agtgagggta tgccatgtca acgattatta tggatttatg tagttacacc cgactaggtt
    11641 taaccgggta tctgttgagt agaggggtta aaaaaagaga aatcaacgac attgaaaccg
    11701 ttgatgacct tgccatagct tgtgattcac agcgcccttc agtggtgttt attaatgagg
    11761 actgtttcat ccatgatgct tctaacagtc agcgcatcaa gcacatcatt aatcaacatc
    11821 ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt tcattttgat gaatatctat
    11881 tggtcagaaa aaatttattg atcagttcta aatcgattaa accggaatct ctcgacgata
    11941 tccttggcga tattctgaaa aaagagacaa cgataacctc ttttttaaat atgccgacgt
    12001 tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg gatggcaggt cagggaacca
    12061 ttcaaatctc tgaccaaatg aatatcaaag ccaagaccgt ttcatcgcat aaaggtaata
    12121 ttaaacgtaa gatcaaaacg cataataaac aggttatcta ccatgtcgtc cgactgacgg
    12181 ataatgtgac taatggtatt tttgtcaaca tgcgctaaca cattctgact ggtggtttcc
    12241 caccagtcag gctgaataag attactctgc tttctccaca aagataccgt cctgatgccc
    12301 tgcttcatta aagaaccaga tgccgagcgg gtagtcttcc agcgaaacca ggtacattgt
    12361 gccttcacta aactcctcaa ctgccagtac cacgccagga cgacgcggac cgccatccgt
    12421 tttgactgtt acccgatcat tcaccttcat cgttttcctc ctgtggcttt gtgccagtgt
    12481 agaacaattt cgtttttctg gcagcgccag gcgcgcgcga gtgctgattt tctcgacggt
    12541 ctatacttaa aagatgccag gcggacttaa cgactggcgg caacaacaga gtaacggttg
    12601 cgaggaaaga tgatgaaaac cgcaaaagag tacagcgata ccgcaaaacg tgaggtcagc
    12661 gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta gcgaaagcga agttcatcgc
    12721 agccagaacg attctgaaca cgttagcgtc gatggacgtg aatatcatac atggcgtgaa
    12781 ttggcggatg ccttcgaact ggatattcat gacttcagcg tctctgaagt gaatcgttga
    12841 atgcacaata aaaaaatccc gaccctgagg gggtcgggat gaaacttgct taagcaagaa
    12901 gcacttaaaa aattcgttac accaggaaat ctgatgtgtt catcacctta tccgcaattt
    12961 ttttcgctga caagaaaata ttcgccatat gaatgattaa cgctacttat gaatagaaat
    13021 gtgacgcacg tcaccccttg gtggctctgg cgtcagcggg atcgctcccg aaaaaaatca
    13081 tctttgataa tttgtgctgt tttgagaatc atcctggtaa ggggagtact gcgcgactat
    13141 gttttcaatt caacaaccac tactggtttt tagcgatctt gatggcaccc tgctggacag
    13201 tcatagttat gactggcaac cagcagcccc ctggctcacc cgtttacgcg aagcaaatgt
    13261 tcccgtcatt ctctgtagca gtaaaacatc agcggaaatg ctgtacttgc aaaaaacgtt
    13321 ggggctacaa ggtttaccgc tgattgccga aaatggcgca gtgatccagc ttgctgagca
    13381 atggcaggag atagacggtt ttccacgcat catctcaggt attagccatg gcgaaatcag
    13441 ccaggtttta aatacgctac gcgagaaaga acattttaaa ttcacgactt ttgatgatgt
    13501 cgacgatgca accatcgccg aatggacggg attaagccgt agccaggcgg cgctgacgca
    13561 gcttcatgag gcgtcggtaa cgctaatttg gcgcgacagt gacgagcgta tggcacaatt
    13621 taccgctcgt ctgaacgaac tgggcttaca gtttatgcaa ggtgcgcgct tctggcacgt
    13681 cctggatgcc tctgccggaa aagatcaggc tgccaactgg attatcgcga cctatcaaca
    13741 attgtcaggc aaacgcccaa ccacacttgg cctgggcgat gggccaaacg atgcgccctt
    13801 actggaggta atggattacg cggtgattgt gaaagggcta aaccgtgaag gggtgcatct
    13861 gcatgatgag gatccggccc gcgtctggcg aacgcagcgt gaaggaccgg aaggatggcg
    13921 tgaagggctg gaccattttt tctccgcccg ttaagtgtta tcgctcgcga atacccgatt
    13981 acgcccagcc tgtttagcga gataaagccg acggtcggcc agtgactgga gttgttcaaa
    14041 atcataatta ccggtttcct cgctgctact tacccccagc gaggcactga tgcgtatcgt
    14101 cgtactctta gcaatcaaca tctctttttc atttaacttc aggcgaatac gttctgcgac
    14161 ttccgcagcc tccgtcagac tcgcgcctgg cagaatcaca caaaactcct caccaccgac
    14221 ccgcccggca acgtcctgca cacgcaagga actgctaatt aatccggcag catgagaaag
    14281 aacacggtcg cccgcctgat gaccaaagcg gtcattaatc gctttaaaat ggtcaagatc
    14341 gacctgaatg acagaaaaag gagttggtgc gtctgacaca atttagcgag cggacgggct
    14401 ttttcgaaca gtgcgccacg gttatataaa cgcgttaagg tgtcgtgcca cgcctgccac
    14461 tgcaacgagc tttgcagaac atacatgttg ctgaccatcc ggcgaatcac ataccaggag
    14521 atgagtaaca tggtggtaaa gagcgcccac agcagggtta atgcaatgct gatactgccg
    14581 aaatcgccgc gcacgccttc gcttaacgta tggacgcgca ccagcacacc gtcgaaatgg
    14641 tccagacgtt cccagctaac atagcgactg tccatacgaa tgccgccccg cgtgtcatgt
    14701 tccattgcct gcgccagcaa ggccagttca cgaggatcaa aaatattccc tgttggatgg
    14761 tcaggattgg aagaggtcaa aaatctcagc ttactgtcat agagctgata ctcaccatcg
    14821 aggtttttat cgatggcgtt tcttaaaaat tgctgcatag tacgcacggg aatactcatc
    14881 cccagcacgc cataccagta gttattactg tctaccggaa cactgacggt aacctgcggt
    14941 tcagtattgc tggcgtgttc cggttgcgaa gtgaaccagc gtaccgcgcg gtgacgattt
    15001 tctcgttgtg aatggccaat aaaccagggt tgggtgacat agccgtaata acgcgttggg
    15061 acattgcgcg taaacaaggt cggctgcgtc gaaacgtaaa atccggcacg cgagacatac
    15121 atcgcctgtt caaccatcga cgaggagttg tgtgccagtc gcagcaagta accaacttcc
    15181 agtgcagcgg taatttcatt gtcgaggctt tcattttcgc gagacaggag attcccctca
    15241 ctgactaatg catccgacac gccattgact ggcagggtgc gccgtcggtt gagttcgatt
    15301 ttccaggcgt gctcgtcgcg atgctgttcg aactcggtta cagcatcacg cagagaggtg
    15361 aaatccagtg gcgctacgag agcttcgcgc atgccattac gcaaaaagat cagtttgtcg
    15421 acgttatact gcaaatgctt atcgagcgcg ttggctacgt tttccagatg attacgctgg
    15481 ctggagatat aggcatcttc cagcactacc acttcacgcc aggtcagcaa ggtggaaaaa
    15541 agcaatacca cgataaagca gagattaacg atatgaccag gccccaggcg gcgtgcgagt
    15601 tttttcaacc agctctggtt ttccattttt gtctcgtgct gcaccctgac gactccccac
    15661 ctgctttttt atgattctgg cataacctgg cgatagcgta ccgcaaaaca gcgataattt
    15721 tataatggtg gcatgataag acgcgactat cattcattgc aggcattggc acaccaggcc
    15781 ggatgtgatc cggcctggtc ttaatgatac cctttcaaac ttcttccgca gaacgactcc
    15841 cctcccccgg aacaagctcg gtttcatgaa aagcttcccg cttgaccccg taaccgtcat
    15901 accaacgaca ctcaaccata ccgctggagt atccagtgac aatcatccgc gggccgccct
    15961 ctttaaccgt aacttcctca ctaaccataa agctcatact cgcctccttt tttcgagtga
    16021 aacttgttca ccttagttga agatggcgaa ttttgcataa tagcctgtgc gagtattaat
    16081 gtgcctgacc gcgcattttc gctacggctt tcactcccag caccacgatg ccgccgatga
    16141 taaatccaag aatcagattt aaaacagtcg gtaatatcat cgccaccact gcactttgct
    16201 gcccggcgaa atgttcaatg gcgtgatgca gcggcgcaat accatgtacc acaatcccgc
    16261 cgccgacgag gaacatcgcc agcgtgccga caatcgataa cgctttcatc agccagggcg
    16321 caatgatcaa taacccttta cctaatgcct gcatcagcgc gctggatttt tccgccagcc
    16381 aataccccag gtcatcaatc ttaacgataa cccctaccag accgtagacg cccacggtca
    16441 ctaccagcgc gatgcctgaa agcaccagca cctgattaag caacggcgct tcggccacaa
    16501 tccccagcgt gatggcgacg atttccgcag acaatataaa atcggtacga atcgcccctt
    16561 ttattttgtc cttttcaaac ttcagcggat cctgcgccgc cagcttctcc agacgctgct
    16621 gactctgcgc cggatcttct ttatgtttac gcgcctccag catatgcagc actttttcta
    16681 ccccttcaaa gcagagaaac gcgccaccaa tcatcaacag aggcgtaatc gcccacggga
    16741 taaacgcact gatgatcagc gccagcggca ccagaatcac tttattgatc agcgatcctt
    16801 tcgccacgcc ccagaccacg ggaagttccc ggttggcccg cacgcctgaa acttgttgcg
    16861 cattgagcga taagtcatcc cctaatacac cggcggtttt cttcgccgcc agtttgccca
    16921 tcacggagat atcgtccagc agtgtggcga tatcatcgag caacgtcagt aaactactcc
    16981 cggccaaaag cattccctct catttttatt gttgaagggt gtaagtatga agcaaaaacg
    17041 gcagctctga cacttgcttc acaggtcaac atttttttaa caatcaagtg caattatatc
    17101 tctcgccagc agaatgattt tcacgtttac tatgaaccac ctttttattt ccaccgtgag
    17161 ggattatgcg tttccgccag ttgttaccgc tttttggcgc gctgtttgcg ttgtatatca
    17221 tttggggctc aacctatttt gtcattcgga ttggcgtgga aagctggcct ccgttaatga
    17281 tggcgggcgt tcgattcctg gcagccggta tttttttgct ggcatttttg ctactgcgcg
    17341 gacacaaact ccccccgcta cgtccgctgc tcaatgccgc gctgattggc ctgttattgc
    17401 tggctgtcgg taatggcatg gtgacggttg ccgaacatca aaatgttcct tccggcatcg
    17461 ccgccgtagt ggttgcaacc gtgcccctct ttaccctgtg cttcagccgc ctgtttggca
    17521 ttaaaacgcg caaactggaa tgggtgggta tcgccattgg gcttgccgga atcatcatgc
    17581 tcaatagcgg tggaaattta agcggcaatc cgtggggcgc gattctgatt ttaatcggct
    17641 cgattagctg ggcgtttggc tcggtttatg gctcgcgcat taccttacct gtagggatga
    17701 tggcgggtgc gattgagatg ctggcggcag gcgtggtgtt aatgatcgcg tcgatgattg
    17761 caggtgaaaa actgacggcg ctcccttccc tttcaggctt ccttgcggtc ggctatctgg
    17821 cgctgtttgg ttcgattatc gccatcaacg cttatatgta tttaatccgc aacgtcagtc
    17881 cggctctcgc caccagctac gcctacgtta acccggtggt cgcggtcttg ctgggtacgg
    17941 gactgggtgg agaaacactg tcgaagattg aatggctggc gctcggcgta attgtcttcg
    18001 cggtggtact ggtcacgttg ggaaaatatc tcttcccggc aaaacccgta gttgcgccag
    18061 ttattcagga cgcatcaagc gagtaaatga atcccctgcg tgtcgatctg cgcgctggca
    18121 ccttcgccgc agatccactc ttccagacgc tcgctaagcg cttcatccgt cagcttctcg
    18181 cgcccacgta acgcgcactc ccagacaatc aatacgcgcc agccgagttc ctgcaagcga
    18241 ctgatatcgc ggcgatcgcg ctcaacattt ttacctatct tctccagcca gaactcggtt
    18301 cgagtcgcag gcactttaaa cagatagcag tgatgatgat gccagaagca gccatgggta
    18361 aatatcacgc agcgatattc atcaacgaca aaatccgggc gtccgggcag actggcgtcc
    18421 tgaacgcgaa acgccaggcc ctgcccggtt aacagactgg cgaggcgctt ctcaatcgcc
    18481 gtatcacgcg tggcaatcgc gcgcatattt ttgctgcgag tggccttatc atgaacgtcg
    18541 gccatgttgt gcctcttgct gacgcaacgc caccgcctgt ttgatttttg gctcaagcag
    18601 ttttgccacc gcggcaaaga ccggcacgac caccgagtta ccgaactggc gataggcctg
    18661 agtgtccgaa accggaatgc ggaatttcgc ttctcccggc gcttcaaaac ccattaagcg
    18721 cgcgcattcc cgaggcgtta accgacgtgg acgatgttgc tgattctgcg gatcgtcaaa
    18781 gtctttctca cccgtggcca tatcccagcc gcgatcgatt aaaatttccg cgccatcttt
    18841 gtaataacgt gcagacagcg tacgcgtgac gctttgcgga ttgttcggat aaaccattcc
    18901 ataaccgaag ccgttaccgc gcgcatgatg ttttttcgca tatcgataga ggtacttcca
    18961 cagcaccggt gtcaggatat atttcgcctc gaccatcggg tccagcagct gcgccagcgt
    19021 cactcgctgc gcagggaaac attcactgat atcacgcagg gtaaaatcgg ctttcagatt
    19081 cagatcgcga cgaaaaccca ccagcacgat gcgttcacgg tgctgcggca ggaaatgttt
    19141 accatcgatg attttcggat cgtccggccc gttatcttct gcatcagcca cgtcatagcc
    19201 cagttcgtcc agcgtctgca tgatgatgcg gaacgttttt cctttatcgt gacttttcag
    19261 gtttttgacg ttttcgagca caaacatcgc cggacgacgc gcgtcgataa tgcgcaccac
    19321 atcaaagaac agcgtgccct gggtatcgca ggcaaaaccg tgcgcccgcc cgagcgagtt
    19381 ctttttcgat acgccagcca gcgaaaatgg ctgacatggg aaaccggcca gtaaaacatc
    19441 gtgttcagga atgtgttgac gaatatgttc cgccgccgcc tcatcactca cgccttcttt
    19501 atggctgagg gtgatgtcgc ggatatcttc attaaaatga tgcgtcgccg ggtcgcaata
    19561 atggttggct ttataagtgc gtaccgcatg tttgttccat tcgctggtaa acacgcactg
    19621 tccgccaatc gattcaaaac cgcgacggat accgccaatt ccggcaaata gatcgataaa
    19681 gcgaaacgca taatgcggat gatgtgccgg tggtttgggt aacaacgttt gcagatgggc
    19741 gaactctttc tcacttaaac ggtgccatgc cgagtcattc gccagcgcac gttttaaaat
    19801 tgccgcgctc cagtgattct cacccacacc attaagctgc gccaccagcg ttttaacatc
    19861 ataaatttgc agcagtttct ccagcattgc ctgtgcggca ttcccggtgc tgtatgaatc
    19921 ggttactgat atattttcct gcatagattt aaccggccat ctgagatggc cgacaggtta
    19981 acataattac agccatttag ggccacgcgc tcagcccacg ggtgaaaacg catctatcac
    20041 cttgtgatcg acaccttcat tctcccccgc cagttcggca ctgagcttcg ccataaactc
    20101 gactaaaaat tgcgcgttgt gctgcgccag ctgcttgcct cttgcggttt gcatggtttg
    20161 cggtagcttc agcaacttag tctggaaatg atccagcgcg tagcgtttat catcaagcgg
    20221 gcgatgctgt gcgagcggat cttcgccatc aaacagcgcc acgcccaatg cccctgaaac
    20281 ggcaaacacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg catcctggac
    20341 gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag cggcaatggc
    20401 atgacaaacg gcctcgattt tctccgccgg aaattgctcg aactcttcac ggagcaggcg
    20461 acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat ttttcgccag
    20521 gctcacaata tcgtggaaat aacaggcggt taaaatcacc agcatatcga catcgtcatc
    20581 tgccgccagc ttctgtgccg tagcccagac gcggcgaaaa tgacacacgt catgggccgc
    20641 gtcctggtgc tgatgatggt ttttcaacca gttttcgaac tgtgcctgcc agtgttgtaa
    20701 gtccatacgc gctccgttgc caaaaagtcg ccaccttagc agtttttatt catagcgtct
    20761 gctacgtgcg ggacgaggtc gggtgtacca ggcaatgctg cctaacaatg ccccgaccag
    20821 agccataaat aaaaatccga ccagtgcccc taaccacttt cccgcggtag agcctaaatc
    20881 gctttcaaat cccgacacgg gggcatcggg gatccggcta atcacccaga tgtaacgcgc
    20941 cattgcccag acgaaatagc cacaccatgt atagaaagcc cacaaagcca gtttgccacc
    21001 agggctgcga gagagtctct cttccatctt tgtcgaaccg tattccattt tgagataacc
    21061 cggaaaattc agacttagta ctattatgtg ataaaagtca catttttcca cattgaatga
    21121 gccagtcaag actcaatcat tttttgaaca aatagataaa ttttgcgcaa cggcagtgaa
    21181 tacaggaaaa a
//
LOCUS       DAPGRN010000084        20935 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPGRN010000084 DAPGRN010000000
VERSION     DAPGRN010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.084"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(15..1664)
                     /gene="actP"
                     /locus_tag="RHW33_003248"
     CDS             complement(15..1664)
                     /gene="actP"
                     /locus_tag="RHW33_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV1646725.1"
                     /translation="MKRVLTALAATLPFVANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1661..1975)
                     /locus_tag="RHW33_003249"
     CDS             complement(1661..1975)
                     /locus_tag="RHW33_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV1646726.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2176..4134)
                     /gene="acs"
                     /locus_tag="RHW33_003250"
     CDS             complement(2176..4134)
                     /gene="acs"
                     /locus_tag="RHW33_003250"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV1646727.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4527..5963
                     /gene="nrfA"
                     /locus_tag="RHW33_003251"
     CDS             4527..5963
                     /gene="nrfA"
                     /locus_tag="RHW33_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV1646728.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6008..6574
                     /gene="nrfB"
                     /locus_tag="RHW33_003252"
     CDS             6008..6574
                     /gene="nrfB"
                     /locus_tag="RHW33_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV1646729.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6571..7242
                     /gene="nrfC"
                     /locus_tag="RHW33_003253"
     CDS             6571..7242
                     /gene="nrfC"
                     /locus_tag="RHW33_003253"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV1646730.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLAIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7239..8195
                     /gene="nrfD"
                     /locus_tag="RHW33_003254"
     CDS             7239..8195
                     /gene="nrfD"
                     /locus_tag="RHW33_003254"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV1646731.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8215..9933
                     /locus_tag="RHW33_003255"
     CDS             8215..9933
                     /locus_tag="RHW33_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV1646732.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWQYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTGQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFVLISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVMLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9926..10309
                     /gene="nrfF"
                     /locus_tag="RHW33_003256"
     CDS             9926..10309
                     /gene="nrfF"
                     /locus_tag="RHW33_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV1646733.1"
                     /translation="MSKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10306..10902
                     /gene="nrfG"
                     /locus_tag="RHW33_003257"
     CDS             10306..10902
                     /gene="nrfG"
                     /locus_tag="RHW33_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV1646734.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11244..12557
                     /gene="gltP"
                     /locus_tag="RHW33_003258"
     CDS             11244..12557
                     /gene="gltP"
                     /locus_tag="RHW33_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV1646735.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12860..13549)
                     /locus_tag="RHW33_003259"
     CDS             complement(12860..13549)
                     /locus_tag="RHW33_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV1646736.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13643..15790)
                     /gene="fdhF"
                     /locus_tag="RHW33_003260"
     CDS             complement(13643..15790)
                     /gene="fdhF"
                     /locus_tag="RHW33_003260"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15371..15373),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV1646737.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15988..17454)
                     /gene="mdtP"
                     /locus_tag="RHW33_003261"
     CDS             complement(15988..17454)
                     /gene="mdtP"
                     /locus_tag="RHW33_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV1646738.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLELLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKAVALPQVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17451..19502)
                     /gene="mdtO"
                     /locus_tag="RHW33_003262"
     CDS             complement(17451..19502)
                     /gene="mdtO"
                     /locus_tag="RHW33_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV1646739.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQVLNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAITEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLEEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19502..20533)
                     /gene="mdtN"
                     /locus_tag="RHW33_003263"
     CDS             complement(19502..20533)
                     /gene="mdtN"
                     /locus_tag="RHW33_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV1646740.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYV
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20552..20827)
                     /locus_tag="RHW33_003264"
     CDS             complement(20552..20827)
                     /locus_tag="RHW33_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646741.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4701 a         5551 c         5738 g         4945 t
ORIGIN      
        1 gggaagggga gagattaatg cgcgcggcct tgctcaacgc caaagccggt ctgggagcgg
       61 ataaactgcg cgcggaacag ttcacgctca cgcgcgcctt ccgctgagtt atcggttgcc
      121 gagaagaacc agatgccgag gaatgccacg gtgatagaga acagcgccgg gtattcatac
      181 gggaagatgg ctttttcgtg accgaggatc tgtacccaaa tcgtcgggcc gaggatcatc
      241 agtaccactg cggtaattag ccccagccag ccgcccatca ttgcgccacg cgtagtcagt
      301 ttcgaccagt acatagaaag cagaatgatc gggaagttac agctcgccgc gatggcaaac
      361 gccagcccca ccataaaggc gatgttctga ttctcaaaca gcacgccgag gataatcgca
      421 atcacgccga ggatcagtac ggtgattttt gatacccgca gctcttcacg ttcggtcgca
      481 ccttttttga agacgttagc gtacaggtca tgcgaaaccg cggatgcgcc tgccagcgtc
      541 agacccgcaa ccaccgcgag gatagtggcg aaagcaaccg ctgaaataaa accgaggaac
      601 aggttgccgc ccactgcatt cgccaggtga acggccgcca tgttgttacc accaatcaga
      661 tggcccgccg cgtctttata ttccggattt gcaccgacca gcatgatcgc gccaaagccg
      721 ataataaagg tcaggatata gaagtagccc ataaacccgg tggcgtagaa cacgctctta
      781 cgtgcttcgc gggcatcgct gaccgtaaag aagcgcatca gaatgtgcgg caagccagcc
      841 gtaccaaaca tcaatccaag ccccagcgac agcgcggaga tcggatcttt caccagccct
      901 cccggcttca tgatgtcgac acctttcggg tgtaccgcca tcgcttcgct gaacagattg
      961 ttgaagctaa agccgacgtg tttcatcacc ataaaggcca taaagctggc accgaacagc
     1021 aacagcacag ctttgataat ttgtacccag gtggtcgcca gcatgccgcc gaacaggacg
     1081 tacatcatca tcagcacgcc gaccagcacc actgcaatgt gatagttaag gccaaacagc
     1141 agctcgatca gtttacctgc gcccaccatc tgggcgataa ggtaaagcgc caccaccacc
     1201 agagaaccac aggccgaaag aatacgaatc ggcccttgtt tcagacgata agaggctaca
     1261 tcggcaaagg tgtaacgccc caggttacgc agacgttcgg caatcaggaa caaaatgatc
     1321 ggccagccca ccaggaagcc cagcgagtaa attaagccgt catagccgga ggtaaacacc
     1381 agtgcggaga tccccaaaaa tgaggcggcg gacatatagt caccggcaat cgccagcccg
     1441 ttctgaaaac cggtgatatt gccgcctgcg gtgtagtagt cgctacgaga acgtacgcgt
     1501 tttgatgccc agtaagtaat gccgagcgta aacacgacga aaatcaggaa cataataatc
     1561 gcctgccagt tcgttggctg gcgctcaacg gccccgctaa tagcatccgc ggcgttagct
     1621 acgaaaggga gtgtggcggc aagcgccgtc agaactcttt tcatgatgct tgtacctcat
     1681 gcaggacttc gttattaaga cggtcgaatt cgccgttcgc ccgccagatg tagataccgg
     1741 tgagaacaaa ggagatcaca atcactccaa caccaatcgg aataccgcgt gtgacgctgg
     1801 tgttcggatt cagcggggtg cccagccagc cgggcgcgaa ggcgatcagt aaaataaagc
     1861 cgatataaac tgccagcata ataatcgaca ggatggtggc aaaccgttgc cgtttttcga
     1921 ctaactccct gaaatgcgca ttgtcttcta tccgctgata aatagtgcca ttcatcacag
     1981 attctccaga ggtaatgtag ggattgtttt aattcccgct cccttatggg agaaggttaa
     2041 cgctcgggta acccttgccg aatgtaggcc ggataaggcg ttatacgccg catccggcaa
     2101 tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta caaaccgtta ccgactcgca
     2161 tccggcaatt gtgggttacg atggcatcgc gatagcctgc ttctcttcaa gcagcttctc
     2221 gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg ttgctggtat cgcccgccgc
     2281 aattttgcgc agaatacggc gcataatttt gccggagcgg gttttaggca gggagtcggt
     2341 ccagtgcagc acgtctggcg tcgccagcgg gccaatctct ttacgcaccc agttgcggac
     2401 ttctgcgtac agttctggtg acggttcctc cccgtgatta agcgtgacgt aggcgtagat
     2461 cgcctgacct ttaatattgt gcggaatacc gacgacagcg gcttcggcga ttttcggatg
     2521 cgccaccagc gccgactcaa tctccgccgt tcccagacga tgaccggaga cgttcagcac
     2581 gtcgtccacg cgcccggtga tccagtaata gccatcttca tcgcgacgcg cgccgtcgcc
     2641 gctgaaatac atatttttga aggtggagaa gtaggtctgc tcaaaacgtt cgtgatcgcc
     2701 aaacagcgta cgcgcctgac ccggccagga gtcggtgatc accagactgc cttcggtggc
     2761 tccttccagc gggttacctt cgttatcgac cagcgccggt tgcacgccga agaacggacg
     2821 tgttgccgaa ccggctttca gctcggtagc gccaggcagc ggggtgatca tgaaaccgcc
     2881 ggtttcggtc tgccaccagg tatcgaccac cggacatttc tcgttgccga tttttttcca
     2941 gtaccactcc cacgcttccg ggttaattgg ctcgcccacg gaaccgagaa tgcgcagcga
     3001 cgaacggtcg gtgccttcga tcgctttatc gccttccgcc atcagcgcgc gaatcgccgt
     3061 gggcgcggta tagagaatat tgacctgatg cttgtccacc acctgtgcca tacgggcagg
     3121 cgtcggccag tttggtacgc cttcaaacat cagcgtggtc gcgccgcagg ccagcgggcc
     3181 gtacagcaaa taactgtgtc cggtcaccca gcccacatcg gcggtgcacc agtagatatc
     3241 gcccggatga taatcaaaga catatttaaa ggtcagcgcc gcgtacacca gataaccgcc
     3301 ggtagtgtgc agtacgcctt ttggttttcc ggtagaaccg gaggtataga gaataaacag
     3361 cggatcttcg gcgttcatct cttccgcctg gtgctgatcg ctggcttgct caaccaggtc
     3421 gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc ccgccagtac gcttcagtac
     3481 caccacatgc tctacgctgg tgacgttcgg gttttttagt gcgtcatcaa cgtttttctt
     3541 cagcggaata ctacgcccgg cgcgcacgcc ttcgtcggaa gtgatcacca accgtgagtt
     3601 agaatcgata atgcgcccgg caacggcttc cggcgagaag ccaccgaaaa ttaccgaatg
     3661 caccgcgcca atacgggcgc aggccagcat cgcaaccgcg gcttccggca ccatcggcat
     3721 ataaatcgcc accacatcac cttttttaat gcccagctcg agcagggtat tggcgaagcg
     3781 gcagacgtcg cggtgcagct ctttatagct gatatgtttg ctctggctgg cgtcgtcgcc
     3841 ttcccagatg atggcggtac gatcgccgtt ttcttgcaga tggcggtcaa ggcagtttgc
     3901 cgccagattc agcgtgccgt cctcgtacca tttaatggac acattaccgg gggcaaagga
     3961 ggtgtttttc accttctggt acggtttgat ccagtcgaga atttttccct gttcgcccca
     4021 gaaggtatca ggtgcgttaa tagattgttg atacatcgcc tcgtactgct gagggtttat
     4081 caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg tgaatttgac tcatgctttt
     4141 gttctccttg taggatgtta ataatatgtg gcataagcgt taaatgtagg ggtattggca
     4201 gttttgttta gtatttgggc gacagatcac gcaaaagtag aattgtgcaa ataaacggca
     4261 gggtaatttt tgaaggtcag caacaaaagt tgattaattc tttgaggaac atgcagttat
     4321 gcatgctgtt gaaaaagagg aagatactga ctaactctaa agtggtattt tacatgcact
     4381 tacaattgat taaagacaac attcacagtg tggttatttg ttacacatag gggcgagcaa
     4441 tgtcatgaca gtgtaggtgc ggttactgtc gtggaaaaca ataaaaaccg ccattgcaac
     4501 aatggcgcaa ttcggatgaa gcccctatga caaggataaa aataaacgca cgccgtatct
     4561 tcagcttatt gattcctttt ttctttttca cttctgttca cgctgaacaa acggctgctc
     4621 ccgcaaaacc tgtaactgtg gaagcgaaga atgaaacctt tgccccgcag catcccgatc
     4681 aatatctctc ctggaaagcc acctcggaac agtcagagcg tgttgacgcc ctggcggaag
     4741 atccacggct ggtgatcctg tgggcggggt atcccttctc gcgcgattac aacaagccgc
     4801 gtggacatgc ctttgctgtg accgatgtgc gtgaaaccct gcgtaccggt gcgccgaaaa
     4861 acgctgaaga tggtccgcta ccgatggcgt gctggagttg taaaagcccg gatgtggcgc
     4921 gtctgatcca gaaagacggc gaagatggct acttccacgg taaatgggcg cgcggcggcc
     4981 cggaaatcgt caacaactta ggttgtgccg actgccataa caccgcctca ccagagttcg
     5041 ccaaaggcaa accggagtta accctttccc gtccgtatgc ggctcgcgcg atggaagcca
     5101 ttggtaaacc ttttgagaaa gccggacgtt tcgaccagca atcgatggtt tgtggtcagt
     5161 gccatgtgga gtattacttc gacggcaaaa acaaagcggt taaattcccg tgggatgacg
     5221 gcatgaaagt cgaaaatatg gagcagtatt acgacaaaat tgccttctct gactggacta
     5281 actccctgtc gaaaacgcca atgctgaaag cgcagcaccc ggaatatgaa acctggacag
     5341 cgggcattca cggtaaaaac aacgtgacct gtatcgactg ccatatgcca aaagtgcaga
     5401 acgccgaagg caaactctac accgaccata aaattggtaa tccgtttgat aacttcgccc
     5461 agacttgtgc gaactgccat acccaggaca aagctgcctt gcaaaaagtg gttgcggaac
     5521 gtaagcagtc gattaacgac ctgaaaatca aggttgaaga tcaactggtt cacgctcact
     5581 tcgaagcgaa agcggcgctg gacgcaggtg cgacggaagc cgaaatgaag ccgattcagg
     5641 acgatatccg tcatgcccag tggcgttggg atctggcgat tgcttcccac ggcattcata
     5701 tgcacgcacc ggaagaaggt ctgaggatgc ttggtactgc gatggataaa gcggcggatg
     5761 cacgcaccaa actggcgcgc ctgctggcga ccaaaggcat cacccatgaa atccagatcc
     5821 cggatatctc gaccaaagag aaagcccagc aggccattgg cctgaacatg gaacaaatca
     5881 aggccgagaa gcaggacttc atcaaaacgg tgatcccgca gtgggaagag caggcacgta
     5941 aaaacggtct gttaagccaa taaccccgtt ccgcctcgca aggggcggaa aacacaatgg
     6001 agtgaatatg agcgtattac gttcgttatt aactgccggg gtgctggcgt cgggcctgtt
     6061 gtggagcctg aacggaatta ccgctacccc tgcggcgcag gcatctgatg atcgctacga
     6121 agttacccag cagcgtaacc cggatgccgc ctgtctggac tgtcataagc cagataccga
     6181 aggtatgcat ggcaaacatg cttccgttat caacccgaat aacaaactgc cggtcacctg
     6241 caccaactgt cacggccagc catcaccgca acaccgcgaa ggggtgaaag atgtgatgcg
     6301 ctttaacgag ccgatgtaca aggttggaga gcagaacagc gtctgtatgt cctgccatct
     6361 gccggaacag ttgcaaaaag cgttctggcc gcacgatgtc cacgtaacca aagtggcgtg
     6421 cgccagctgc cattccctgc atccacagca agatacgatg cagacgttaa gtgacaaagg
     6481 acggattaag atttgcgtcg attgccacag cgatcagcgc accaatccga actttaaccc
     6541 ggcgtctgtt ccgttgctta aggagcagcc atgacctggt ctcgtcgcca gtttctcacc
     6601 ggcgtcggcg tgctggcagc cgtcagcggc accgcaggac gcgtggtggc gaaaacgttg
     6661 aatatcaatg gggtgcgtta cggcatggtg catgacgagt cgttatgcat cggctgtacc
     6721 gcctgtatgg atgcttgtcg ggaagtgaac aaggtgccgg aaggcgtctc gcgcctggcg
     6781 attattcgta gcgagccgca gggcgaattt cctgatgtga aatatcgctt cttccgtaag
     6841 tcttgccagc actgcgatca tgcgccgtgc gttgacgtct gcccgaccgg tgcgtctttt
     6901 cgcgatgccg ccagtgggat tgtcgatgtt aatcctgatc tctgcgtcgg ttgtcagtac
     6961 tgcatcgccg cctgtccgta ccgtgtgcgc tttatccatc cggtcacgaa gacggcggac
     7021 aaatgcgatt tctgccgtaa gaccaatttg caggccggta agctgcccgc gtgcgttgag
     7081 gcttgcccga cgaaggcgct gacgtttggc aatctggacg atcctaacag tgagatttcg
     7141 caactgctgc gccagaagcc tacttaccgc tacaagctgg cgctgggaac caaaccgaag
     7201 ctgtaccgcg taccgtttaa atacggggag gtgagccaat gacgcagact tccgcatttc
     7261 attttgaatc gctggtgtgg gactggccga ttgccatcta cctgtttttg attggtattt
     7321 ctgctggttt ggtgacgctg gctgtgctgt tacgtcgctt ctacccgcag gcgggcggtg
     7381 cagacagcac gttgctgcgc accacgctga ttgtcggacc tggcgcggtg atcctcggtc
     7441 tgttgatcct cgtcttccac ctgacaagac cgtggacctt ctggaagctg atgttccact
     7501 acagttttac ctcggtgatg tcgatggggg tgatgctgtt tcagctttac atggtggtgc
     7561 tggtgctgtg gctggcgaaa atctttgaac atgatttgct tgccctgcaa caacgctggt
     7621 tgccgaagct ggggatcgtg caaaaggttc tgagcctgct gacgcccgtt catcgcggac
     7681 tggaaacatt gatgctggtg ctggcggtgt tgttgggggc ttataccggc tttctgctgt
     7741 cggcgctgaa atcgtatcca ttcctcaata acccgatcct gccggtgctg ttcctcttct
     7801 ccggcatctc gtccggtgcg gcggtggcac tgatcgccat ggcgatacgc caacgcagta
     7861 acccgcattc cacggaagcg cagtttgtac accgtatgga aatccccgtg gtatggggtg
     7921 aaatcttcct gctggtggcg ttttttgtcg gtctggcctt gggcgatgac ggtaaagtgc
     7981 gtgcgctggt ggcggcattg ggcggcggtt tctggacgtg gtggttctgg cttggtgtcg
     8041 ccgggctggg gctgattgtg ccaatgttgc tcaaaccgtg ggtcaatcgc agttccggca
     8101 ttcctgccgt gctggcggcg tgtggagcca gcctggtcgg cgtgttgatg ttgcgctttt
     8161 tcattctcta cgccgggcag ttaacggtgg cgtaagccag aacagaggtg gtttctggac
     8221 gtattccttc ctgaagtcgg ttttctggcg ctgttgttaa gtctcggggt caacgtgttg
     8281 accccgttga cggcctttgc gggagtgcgg ttgcgctggc ctgccatgat gcgactcact
     8341 tgcatcggca ttctggcaca gttcgcgctc ctgctgctcg cctttggcgt actgacgtat
     8401 tgttttctca tcagcgattt ctcggtcatt tatgtcgccc aacatagcta cagcctgctg
     8461 tcgtgggaac tcaaactggc ggcggtgtgg ggcggtcatg aaggttcgct gctgctttgg
     8521 gtgctgctgc tttccgcctg gagcgcgctg tttgcctggc aatatcggca gcaaaccgat
     8581 ccgctatttc cgctgacgtt agccgtttta tctctcatgc tcgccgcact gctactgttt
     8641 gtagtgctgt ggtccgatcc cttcgtgcgg atatttccac cagcaatcga aggccgcgat
     8701 ctcaatccga tgctgcaaca tcccggtctt atctttcatc caccgctgct ttaccttggc
     8761 tatggcggtt tgatggtagc ggcgagcgtg gcgctggcga gtttactgcg cggcgagttt
     8821 gatgcggcct gcgcccgaat ttgctggcgt tgggcactac ctggctggag tgcattaact
     8881 gcggggatca tcctcggttc ctggtgggcc tattgcgaac tgggctgggg cggctggtgg
     8941 ttctgggatc cggtggaaaa cgcctcttta ctaccctggc tttctgccac tgcgctgctg
     9001 catagtttat ccctgacagg ccagcggggg attttccgcc actggtcgct gttgttggcg
     9061 atagttactc tgatgctgtc gctgctgggc accttaattg tccgttctgg cattctggtt
     9121 tcggttcatg cgttcgcgct ggataacgtc cgcgccgtgc cgttgttcag tctgtttgta
     9181 ctgattagcc ttgcgtctct ggctctgtat ggctggcgag cgcgggacgg tggcccggcg
     9241 gtgcgttttt cggggttatc gcgggaaatg ttaatcctcg ctacgctgtt gctgttttgc
     9301 gcagtgctac tgatcgtgct ggtgggaacg ctttatccga tgatttacgg cctgctgggc
     9361 tggggacgcc tctccgttgg cgcaccgtat tttaaccgcg cgacgttacc gtttggtctg
     9421 ttgatgttgg tggtgattgt gctggcgacg tttgtctctg gtaaacgcgc gcagcttccg
     9481 gcgctgctgg cgcatgcggg cgtgctgtta tttgccgcag ggatcgttgt ctccagcgtc
     9541 agccgtcagg agatcagcct gaatttacag ccgggccagc aggtgatgct ggcaggatac
     9601 accttccgtt ttgagcgcct cgatctacaa gccaaaggca attacaccag cgaaaaagcg
     9661 atagtggcgc tgtttgacca tcagcaacgc attggtgaat taacgccgga gcggcgtttt
     9721 tacgaagcac gtcgtcagca aatgatggaa ccgtcaattc gctggaacgg catccatgac
     9781 tggtatgcgg tcatgggtga aaaaaccgga gcggatcgtt acgcttttcg tttgtatgta
     9841 caaagcggtg tgcgctggat ctggggggga ggactgttga tgattgcagg cgcattatta
     9901 agcggatggc gggggaggaa gcgcgatgag taaaggactt ctcacgctac tgctgttatt
     9961 tacctgtttt gcgcgcgctc aggttgtaga cacctggcaa ttcgccaatc cgcaacaaca
    10021 gcaacaggcg ttaaatattg ccagccagtt acgttgtccg cagtgccaga atcaaaactt
    10081 actggaatcc aacgcgccgg tggcagtcag tatgcgccat caggtttaca gcatggtggc
    10141 ggaggggaaa agtgaagtcg aaatcattgg ctggatgacc gaacgctacg gagattttgt
    10201 tcgctataac ccaccgttaa cgggtcagac gctggtgttg tgggcgctgc cagtggtgtt
    10261 gttactgctg atggcactga tcctctggcg agtgagggcg aagcgatgaa acagccaaaa
    10321 ataccggtga aaatgctgac aacactcacg atattgatgg tattcctctg tgtcggtagt
    10381 tatctgttaa gcccaaaatg gcaggcggta cgtgcggagt atcagcgtca gcgcgatccg
    10441 ctacatcagt ttgccagcca gcaaaccccg gaagcgcagc ttcaggcatt acaggataaa
    10501 atccgcgcta atccacaaaa cagcgaacag tgggcgttac tgggcgagta ctatctgtgg
    10561 caaaacgatt acagcaattc gctgctggcg taccgtcagg cgttgcaact acgtggtgag
    10621 aacgctgaac tgtatgcggc gctggcgacg gtgctttatt accaggccag ccagcatatg
    10681 accgcccaga ctcgcgcaat gatcgacaaa gccctcgcgc tggactctaa cgaaatcacc
    10741 gctctgatgc tgctggcttc cgatgcgttt atgcaggcga attacgcgca agccatcgaa
    10801 ctatggcaaa aagtgatgga tctcaactca ccccggatca accggacaca gctggtcgag
    10861 tcgattaata tggcgaaact gttgcagcgg agatcggatt aatcatctgg tgatgaagat
    10921 tatgttattg agctgcataa aaaataatcg aatgaacata tgccaaaaat aatcaccaat
    10981 cggtattatt gcagattaaa caaataaaaa tctttccata acaaatggtt attcattaat
    11041 cctgccataa actcgcgtta ttatgcatta atgcagcgaa aagctctgtt gttaaagggt
    11101 tgcgcaacat accgcgcaaa tgatactgat cataagcgtt aaaaaaatct acaaaccaac
    11161 gcaacacaat tcatgccctg gcagtatgtc acgttctcgc gtttctgaac ggggaacggc
    11221 gctccattga ggaagttatt ctgatgaaaa atataaaatt cagcctggcc tggcagattc
    11281 tgtttgctat ggtgctgggc attctcctgg gaagctacct gcactaccat agcgacagcc
    11341 gcgactggct ggtcgtcaat ttgctctctc cggcgggtga tatcttcatc catctgatca
    11401 aaatgattgt tgtgccgatt gtgatctcca cgctggtggt gggtatcgcg ggtgttggtg
    11461 atgccaaaca gctcgggcgt attggcgcga aaaccattat ctacttcgag gtgatcacca
    11521 ccgttgccat cattttgggg atcactctgg cgaacgtctt ccagcccggt gccggggtgg
    11581 atatgtcgca gttggcgacc gtcgatatct cgaaatatca gagcactacg gaagcggtac
    11641 aaagcagttc ccacggcatt atgggcacga ttttgtcgct ggtgccgacg aacattgtgg
    11701 cgtcgatggc gaaaggcgaa atgctgccga tcatcttctt ctcggtgctg tttggtctgg
    11761 ggctttcctc cctgcccgcg acgcatcgtg aaccgctggt gaccgtgttc cgctccatct
    11821 ctgaaaccat gtttaaagtg actcacatgg tgatgcgtta tgcgccggtg ggtgtgtttg
    11881 cgctgattgc ggtgacggtg gctaactttg gtttctcttc tctgtggcca ctggcgaaac
    11941 tggtgctgct ggtgcatttc gccattctgt tcttcgcgct ggtagtgctg ggaattgtgg
    12001 cgcgcctgtg cgggttaagc gtctggatcc tgattcgtat tctgaaagat gagctgattc
    12061 tggcgtactc cactgccagc tctgaaagcg tgctgccgcg aattattgag aagatggaag
    12121 cctacggagc accggcgtcg atcaccagtt tcgtggtgcc gaccggttac tcttttaacc
    12181 ttgatggttc gacgctgtat caaagtattg ccgctatttt catcgctcag ctgtatggca
    12241 ttgacctgtc catctggcag gaaatcattc tggtgctgac gctgatggtg acctcgaaag
    12301 ggattgctgg cgtgccgggc gtgtcgtttg tggtgttgct ggcaacgctg ggtagcgtag
    12361 gtattccgct ggaaggtctg gcgtttattg ccggtgttga ccgtatcctc gatatggcgc
    12421 gtactgcgct gaacgtggtg ggtaatgcgc tggcggtgct ggtgattgcc aagtgggaac
    12481 acaaatttga ccgcaaaaag gcgctggctt atgagcgtga agtgctgggc aaatttgata
    12541 aaactgcgga tcaataattg aagattgccg gggatatccc ccggcaatgt gtggatgcct
    12601 gatgcgacgc tggtgcgtct tatcaggcct acgtcagaca gcgcaatagc ctgatttagc
    12661 gtgattttgt aggtcggata aggcgtttac gccgcatccg acatcaacgc ctgatgcgac
    12721 gcttgccgcg tcttatcagg cctacgccag acagcgcaat agcctgattt agcgtgattt
    12781 tgtaggtcgg ataaggcgtt tacgccgcat ccgacatcaa cgcctgatgc gacgcttaac
    12841 gcgtcttatc gggcctatct taaccgttgg ttaatttttc aaactcttca cacccggtat
    12901 caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg ttcttctcga
    12961 taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc
    13021 gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc
    13081 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct
    13141 ccagatcttc cgaggcgttt tctaacagcg aaattgcctt tggataatcc ggtttacccg
    13201 cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
    13261 ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt
    13321 tgatctgcgc caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc
    13381 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
    13441 cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttgg
    13501 cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac
    13561 gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa
    13621 ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact
    13681 tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc
    13741 gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca
    13801 gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac
    13861 gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc caaaccaatg
    13921 cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt
    13981 catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac
    14041 caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg
    14101 ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa
    14161 acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc
    14221 ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact
    14281 catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt
    14341 cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac
    14401 gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg
    14461 gtaaaataac atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca
    14521 gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt tgtagcggat
    14581 cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc
    14641 tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct
    14701 cgcggttagc cggatctttc acatactggt atcccggata cgtatccggc agcgcgccca
    14761 tatcgcaggc accctgaacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct
    14821 taccgaggtt accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct
    14881 ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca
    14941 tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca accgactccg
    15001 gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga
    15061 atgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga
    15121 tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc
    15181 gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca
    15241 cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa
    15301 tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg
    15361 atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg
    15421 caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct
    15481 ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca
    15541 gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg
    15601 ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct
    15661 tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc
    15721 cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga
    15781 cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt
    15841 atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg gcactattta
    15901 ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg
    15961 cgtgacagcc atcacgcggt agacatttta ttttttctcg acgacgggag ccgcctgata
    16021 cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat
    16081 cagtaatgac atctcttcgg caagcaccgg caaccgggct tcggtagcct gtaagcggct
    16141 ggtcaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac
    16201 gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc
    16261 aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc
    16321 ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag
    16381 acccgggata aagttgaact ggcgactggt ttttttgaat aaggtatcca ggtggatggc
    16441 atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc gcgcggaatc
    16501 cacctgatct aatgacgcct gaacatacca gcgcatcgct tgcagatccg ggcgtctggc
    16561 gagcaactca taagagagcg tcgccggaat gccggtctgt acttgcggta atgccaccgc
    16621 tttgatctcc ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctcgcgt
    16681 ttcggtgatt tgccctttaa cggcagcaat ttgtttatcg accgccagaa tctgtgcccg
    16741 cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct ggtgcgcttt
    16801 taccgcgtaa tcaatcacat cgcgagtttg ttctaacagc tcgagcatct gatagctggc
    16861 ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt
    16921 ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc
    16981 ccacaaatca agatccagtc cggcaaacaa accgactgtg gcttccgtat agtacggccc
    17041 gtccataccc agtgctggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac
    17101 gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg catctaacaa
    17161 atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc
    17221 acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt tccaccactg
    17281 cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg
    17341 tttgagctgt tgatgaggcg cagaatcctt gcgtaccagg gcacagccgg aaatcagcgt
    17401 cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg
    17461 gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg
    17521 gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag
    17581 gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca
    17641 gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc
    17701 agcgcctgcg ccgagttcca ggtggcatcc caggcgtgaa gaagatcgcg gccctgacga
    17761 ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc
    17821 agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag acctatacga
    17881 atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta
    17941 cttagcatac ccagcgcacc ggccagtttt tgcggcagcg tgcgcgcttc actttcaggc
    18001 cagacaaagg tgtaaatcac cgcagacacc acggtaccaa tgatgatacc cagggcgcga
    18061 tcgcgaattt cctccaggtc gtacaccgga ccaaaaacgt tttcgagcgt ggcgagcgcg
    18121 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatccat
    18181 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18241 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18301 cgcagcacca tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc
    18361 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa
    18421 caggcgagca gtgtttttac cgcgtagcgc atatagtctg gattggtaaa agcatcggcg
    18481 gccattgatg gcggtttggc tgcgggcgtt ggcggcgtat tcgggtccat ctgacccagt
    18541 tgtaacagcg tctggcagat attctccagg ttacattccc gtgccgccat cgcttcactt
    18601 tcactgatcc gccagtcgct ttgccagcac tgaccttcgg taatggcatg ctgcagcttg
    18661 ttgatttctg aagctaattt ttgtcggaat tcaataattg cctgagaatc agcaaaagag
    18721 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18781 caccatgcac tctgggttcg ccagttggca tcgtccgcga ggcaaaagac attgagtttt
    18841 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc
    18901 aggtggctaa tggcatcatc aagccgatca ttaagcacct gatgcatttg cgaaatggca
    18961 cgactgggaa accacagcac gccgattaac gtcatcaata acgtcgggta gaggccgaca
    19021 acgatacacc acagggttaa gcgcacgacc acttccggat agtcgagcat ggcggggaag
    19081 gtttgcccgt aaatagcgac aatggcgacg gcgaaaaaga ccagccccaa ccgatgggtg
    19141 cgcatcaaaa acatgcagcc catcaggatc ggtccggcga tgatcagccg gatcagaggc
    19201 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19261 acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19321 actgccagcg ataacgccac gaaagggatc tcaaaggtca tcgagatcag aatcaccagc
    19381 aggcagccta cccagagttg cacggtctgc ggcacgcgac ctggtcgccg ctcgcttaac
    19441 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19501 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19561 gtttatcaac catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac
    19621 gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19681 caattgaatc cactttaccc tcgaaagttt tgccgctgtc actcatcagg cgaatcgttg
    19741 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc
    19801 agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19861 aggttttgag ggaaatgacc cggccatcaa acggtgcgcg aacggtcgcc atttccagat
    19921 gcagtttggt cagggcaata tccgcttcga ccgccgcacg ctgggcaact aatgcatcca
    19981 cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt
    20041 ctgcggcgcg ctgcgcggtc ctcgcacggt caacgtcttc cgctgagaca aaaccttctt
    20101 tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt
    20161 tttctaccgt ggcattaacc gagtcggcac caaactgttg cgcgtcaacg ctacgctggg
    20221 tgagcataat ttgtttatcc agcgccgcga gggaggcttc ggctttcgcc agattggctt
    20281 cgtacgggcg cgggtcgatg cggaacagca aatcgccctg tttgactgcc tggttgtcgg
    20341 tgaccgccag ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg
    20401 acacgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg
    20461 caaccagcgc caacgcaacc actaacagag cagggaattt actgcgagga gcttttttcg
    20521 gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg
    20581 cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgtcc
    20641 gctgaataat tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc
    20701 agctgggata ataagcgccg atcaccggga ttgccggaga taatgaacat cccgaaacca
    20761 tgaagacggg aattatccag gctgtttttt tgagttgcat cgccatgcga gagagaactg
    20821 ttggcatatt aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcactatcc
    20881 attgaaggcg tcagtatggc tattttcttg atgaataaaa atagctttat tcagc
//
LOCUS       DAPGRN010000085        20644 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPGRN010000085 DAPGRN010000000
VERSION     DAPGRN010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.085"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..361
                     /locus_tag="RHW33_003265"
     CDS             <1..361
                     /locus_tag="RHW33_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HDV1646742.1"
                     /translation="YVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSP
                     FDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSV
                     ELHDKVIGHYLNIKHYQ"
     gene            377..784
                     /gene="pgrR"
                     /locus_tag="RHW33_003266"
                     /pseudo
     CDS             377..784
                     /gene="pgrR"
                     /locus_tag="RHW33_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
     gene            1121..2734
                     /gene="mppA"
                     /locus_tag="RHW33_003267"
     CDS             1121..2734
                     /gene="mppA"
                     /locus_tag="RHW33_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV1646743.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAKKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2785..3816)
                     /gene="ynaI"
                     /locus_tag="RHW33_003268"
     CDS             complement(2785..3816)
                     /gene="ynaI"
                     /locus_tag="RHW33_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV1646744.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            4060..4317
                     /locus_tag="RHW33_003269"
     CDS             4060..4317
                     /locus_tag="RHW33_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV1646745.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQLSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(4367..5317)
                     /gene="uspE"
                     /locus_tag="RHW33_003270"
     CDS             complement(4367..5317)
                     /gene="uspE"
                     /locus_tag="RHW33_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV1646746.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(5469..6221)
                     /gene="fnr"
                     /locus_tag="RHW33_003271"
     CDS             complement(5469..6221)
                     /gene="fnr"
                     /locus_tag="RHW33_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV1646747.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(6416..6931)
                     /gene="ogt"
                     /locus_tag="RHW33_003272"
     CDS             complement(6416..6931)
                     /gene="ogt"
                     /locus_tag="RHW33_003272"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV1646748.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(6942..8468)
                     /gene="abgT"
                     /locus_tag="RHW33_003273"
     CDS             complement(6942..8468)
                     /gene="abgT"
                     /locus_tag="RHW33_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV1646749.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(8505..9950)
                     /gene="abgB"
                     /locus_tag="RHW33_003274"
     CDS             complement(8505..9950)
                     /gene="abgB"
                     /locus_tag="RHW33_003274"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV1646750.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTSNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(9950..11260)
                     /gene="abgA"
                     /locus_tag="RHW33_003275"
     CDS             complement(9950..11260)
                     /gene="abgA"
                     /locus_tag="RHW33_003275"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV1646751.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGIIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVEIRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            11436..12344
                     /locus_tag="RHW33_003276"
     CDS             11436..12344
                     /locus_tag="RHW33_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1646752.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            12674..13237
                     /gene="smrA"
                     /locus_tag="RHW33_003277"
     CDS             12674..13237
                     /gene="smrA"
                     /locus_tag="RHW33_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV1646753.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13258..14490)
                     /gene="dgcM"
                     /locus_tag="RHW33_003278"
     CDS             complement(13258..14490)
                     /gene="dgcM"
                     /locus_tag="RHW33_003278"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV1646754.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            14745..15728
                     /gene="zntB"
                     /locus_tag="RHW33_003279"
     CDS             14745..15728
                     /gene="zntB"
                     /locus_tag="RHW33_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV1646755.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            16003..16173
                     /locus_tag="RHW33_003280"
     CDS             16003..16173
                     /locus_tag="RHW33_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017372341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646756.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRA"
     gene            16206..17579
                     /gene="dbpA"
                     /locus_tag="RHW33_003281"
     CDS             16206..17579
                     /gene="dbpA"
                     /locus_tag="RHW33_003281"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV1646757.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIDQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(17708..18643)
                     /gene="ttcA"
                     /locus_tag="RHW33_003282"
     CDS             complement(17708..18643)
                     /gene="ttcA"
                     /locus_tag="RHW33_003282"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV1646758.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(18819..19253)
                     /gene="uspF"
                     /locus_tag="RHW33_003283"
     CDS             complement(18819..19253)
                     /gene="uspF"
                     /locus_tag="RHW33_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV1646759.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(19394..20527)
                     /gene="ompN"
                     /locus_tag="RHW33_003284"
     CDS             complement(19394..20527)
                     /gene="ompN"
                     /locus_tag="RHW33_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV1646760.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         5308 a         4934 c         5107 g         5295 t
ORIGIN      
        1 atacgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca ggctccggaa
       61 gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc gtcttatgag
      121 cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc tgtatgaatc
      181 ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa ttgagcggca
      241 taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt cgttctcaaa
      301 atcggtggaa ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata
      361 agttggagtc attaccccaa tggctattgt tggctcaccg gattattttt ctcgccgaag
      421 tgttccaacg tcagtgtcac aattaataga tcatcaggca attaatttgt atcttcccac
      481 atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaagttc gtgttcgcat
      541 ggaaggtcag cttttactga atacgataga cctgatcatt gatgctgcaa ttgatgggca
      601 tggattggcg tatctacctt atgatcaggt tgagcgggct attaaagaaa aaaaactgat
      661 acgtgttttg gataaattca caccagattt acccggttat cacctgtact atccacaccg
      721 tcgacatgct ggctcggcat tctcattatt tatagatagg ctgaagtata aaggtgctgt
      781 ttagcactac ttgctgatac attaatttaa ttcttctctt aacgtattct cagttccttt
      841 caacgttttg gttatttttt attcttcgta caatggcgac agatgctgat tatgataccg
      901 aaaactggtt tgaacgtgcg aagcccgaac gtagtgttcg gtgttctatg tgctttgccg
      961 cattttggcg tctattattt acactaaatc tgatttaata tattgataca taaaacattt
     1021 tatgcttcct gtgtcacttt tttgatggaa gttgtttgca tttctttaag gcgaaacaaa
     1081 taattacgca tcaattttaa tgtcggttag agggaaactt atgaagcact ctgtttcagt
     1141 cacgtgttgt gcgctgttgg tcagcagcat ttctctttcg tatgctgcag aagttccgag
     1201 cggcacagta ctggcaaaga agcaggagct ggtgcgccac attaaagatg agcctgcgtc
     1261 gctggatccc gctaaagccg tgggcctgcc agagattcag gtcattcgcg atctgtttga
     1321 aggactggtg aatcagaacg agaaagggga gattgtcccc ggcgttgcga ctcagtggaa
     1381 aagtaatgac aaccgtatct ggacttttac cctgcgcgat aacgcaaaat gggcggatgg
     1441 cacaccggta acggcgcaag attttgtcta cagctggcag cgtctggtgg acccgaaaac
     1501 gctatcgcca tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac aggcgattat
     1561 tgatggtaaa gctacgcctg accagcttgg cgtcaccgca gttgatgccc atactttgaa
     1621 aattcagctt gataaaccgt tgccgtggtt tgtgaattta accgctaact ttgccttctt
     1681 cccggtgcaa aaagccaacg tagaaagcgg taaagagtgg acgaaacccg gaaatctgat
     1741 cggcaatggc gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg tcgtggtacc
     1801 gaatacccat tattgggata acgccaaaac ggtactgcaa aaagtgacct tcctgccaat
     1861 taatcaggaa tccgcagcca ctaagcgtta cctcgcgggg gatattgata tcaccgaatc
     1921 cttcccgaaa aatatgtatc agaagctgtt gaaggatatt ccggggcagg tttatacgcc
     1981 gccgcagctc gggacctatt attatgcgtt taacacgcaa aaagggccga cggcagatca
     2041 gcgcgttcgt ctggcattaa gtatgacgat agatcgccgc ctgatgaccg aaaaagtatt
     2101 agggacgggc gaaaagccag cgtggcattt tacaccagat gttaccgccg gatttacgcc
     2161 ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg caaaaacttt
     2221 gttgagcgca gctggttatg gtccgcaaaa accgctgaag ctgacgcttt tgtataacac
     2281 ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa agaaccttgg
     2341 cgtagatgtt aaattgcaaa atcaggaatg gaaaacctat atcgatagcc gtaacaccgg
     2401 caattttgat gttatccgcg cctcatgggt gggggattat aatgaaccct ccactttcct
     2461 gacattatta acgtcaacgc attcaggaaa tatttcacgc tttaacaatc cggcatacga
     2521 caaagttctg gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg ccgattacaa
     2581 cgcggcagaa aaaatcctca tggagcaagc accgattgca ccaatttatc aatataccaa
     2641 tggacgatta atcaaaccgt ggctgaaagg ttatcccatt aataaccctg aagatgtggc
     2701 gtacagtcgg actatgtata ttgtgaagca ttgatgtgat gggaactggc gttacccttg
     2761 tggataacgc cagtgatgtc tgatttagcg accctgttcc ggcggtgtaa tgttatccat
     2821 atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa tatcgataat
     2881 cttcaaataa acgtcttgct gtgcagcaag ccactcagcc cataccgtgg ttttggtaaa
     2941 gcaataaacc ataatattca atgacgagtc agcaaactgg ttgaaataaa ccagtaaggt
     3001 ttgtcgctgg tcgatggccg ggtgattttt cagcatctca cgtacggctt cgacaataac
     3061 gcccactttt gccgcatcct cataacgtaa accaatggtc gtggtaatgc ggcggttggt
     3121 cattcgtcct gggttttcta cgctgatcga cgaaaacagc gagttcggta cgtacaatgg
     3181 acgattatca aaggtcgtaa ttttggtaat tcgccagcca atttccgcta ctgtaccttc
     3241 gatatttctg tccggtgaac ggatccagtc gccaatactg aaaggacggt cgaaatagag
     3301 catgatcccg gaaaagaaat tactcagaat atctttgccg gccataccga ctgccagacc
     3361 accaatacca ccaaaggtca gcaagccaga aaggctcatg ccgaaatgtt cgccataaag
     3421 cagaacaagc accacaataa tggtgatttt gataatgcgc gacataatcc gcgcactggt
     3481 gatatcgcga ccttttttaa tctgctgttt ttcaaactga ttaatcagca aaaatagctt
     3541 aatcgtcaga ataaccgcaa tcagggacgt acagataaaa tcgataacgc ctggagtgat
     3601 aaatttgagt ttatagtttt ctataacata attaataatg ctaccaacag cactgataat
     3661 tatggtgtag attaaaaatt gcaccgcatg gaataaaaaa ccttttcttt tacgatttcc
     3721 gcggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag
     3781 attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct cctccagata
     3841 attgtcgtca tgccggaaac ccctggcggg gctattttac cgcgacaatt cattcagatc
     3901 atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt tgattgtaaa
     3961 tccagctaag aggtgaggtt ttcagagcag acaacggtga aatgtcatgg tattgttacg
     4021 tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc gaaactgaag
     4081 tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat tgcggcgtcg
     4141 gttgtgactc gcgcgactat cggcggcgtt atagaacagt acaatattcc gctgtctgag
     4201 tggacgacat caatgtatgt gattcagtta tcgatgattt ttgtttatag cctggtcttt
     4261 actgtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga gcagtaagta
     4321 aaaaataggc ccgataactc gggccttgtc agttattgaa gagtcgttaa tcgtcttctt
     4381 cgtcatccag ttcaacgggt gtctgatact ggtcaggttt aatgaccaac agatcgcagc
     4441 gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc
     4501 gtcctaccgt gcccagaacc acaatccccg cctgtaagtg ctccgccaaa tcaggaatca
     4561 cctcttctgg cagacctttt tctacgtgcg tcatgttttc attaatgccg aatttctgcc
     4621 gcagagcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa acactcgggt
     4681 caaattccgg cagttcaatc gcgatattaa ttggcgttac tgggtaagcg ccaaccagat
     4741 gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc agtttttcat
     4801 tgagcgcatt atgatacggc tcttcactgg cgagattcac cgccaccagc gccttacctc
     4861 cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg cgtaacagat
     4921 gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc gccattttta
     4981 gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa ggacggttat
     5041 gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt gcctgctcgt
     5101 ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg
     5161 agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct ttaattttgc
     5221 caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg tcctggttag
     5281 gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt acaacaactg
     5341 tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca gtgatgaacc
     5401 ttctgccaga tcaataaatc agaaaaattt aatgatatga cagaaggata gtgagttatg
     5461 cggaaaaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc atcgttattt
     5521 tcgatggtga tgtatttacc tttgactgcc agcataccgc ttttctggaa gcgacccagc
     5581 agacggctga tggtttctac cgtcaggccc agatagttac cgatatcgcc acgagtcatc
     5641 gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga caggttgtag
     5701 atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaggat catgtcctga
     5761 tcgcctttga tttcaccgct catcagacgc atcatttgct gacgcagatt cggcatttta
     5821 ccggacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt ttccagcgcc
     5881 tgcgcgaagc tcgggtgatg gccgctgccg atggcgtcaa atcccaccag gtcgcctgct
     5941 aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact tttaatcgta
     6001 ccggagcgga tggcataaag cgatttaagt tcatcaccag ccttaaacag cgtctggcct
     6061 ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc atgttcgttg
     6121 agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat agcacaaccg
     6181 ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct caagccgtaa
     6241 ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc aaaccttaac
     6301 agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaaa cccttaagtt
     6361 gttaagggta actttacata aaagtgtgaa caagctggca caaattgttt aatgtttaca
     6421 gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg cctgcatatc
     6481 cagtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg ctgatgggat
     6541 tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcccaat tgttcagcca
     6601 gttggccgta atgcattacc tgcccgcagg ggatagtgcg tagtgttttc cagacttcgc
     6661 gctgaaatgg cgtcccccct gtggcagtgg gaagcgtatc aataatgcta agattaccgg
     6721 caaaataatc acgaagcttg tcgcttaaac cgcctgggtt ggtggcagaa atgcgctcat
     6781 agccttcttt gcgataatgg atgtccagca gctgcaccat gcgttcactg tactcttccc
     6841 attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga cccagtggcg
     6901 tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa acgtgggtaa
     6961 atacccggac cgatcggcag accgacaaga taccacgcca gcaacatcag cagccatacc
     7021 accaaaaaga taagcggata gggcaagact aacgaatagt aagtacccag tttcgcgtct
     7081 ggtttgtagc gttgcaggaa tccaagaaac agtggaacaa aaggagatac cggcgctaaa
     7141 ggcaatacgg atgagtcggc aatacgaaag aggatttgcg caaatgccgg gtgaaagcca
     7201 agtagcataa acattggtac gaaaatgggg gccagaatcg accagattgc ggaaccgctg
     7261 gcgataaaca tgcataagaa agaggaaagc aacgccagac cgacaaacgc cgggatgccg
     7321 ctaagccctg aactttccag tatatcggtc agccccacgg cgatgaattt ccccatgttg
     7381 ctccagttaa acatggcgac aaactgggcg aggggaaaaa ccatcacgat aaatcccgcc
     7441 atctctttca tcggttcaat cattaaatgc ggtaaatccg cctgacgtcg aattgtgcgg
     7501 gtagcgatgc cataagccag cgagacaaca aagaaaaaaa gaatgatcag tggcacgata
     7561 cctttaataa agggtgatgg catcacggtg tgattaatcg gatcgcgcaa tatcccgttt
     7621 tccgggatca ccatcagcgc aatcgcagca ataaaaagta gcgatacgac acctgctatg
     7681 cgtaaaccaa aacgctgact ttcggtcaat gtctgcagtt tctcatcgct gtttccctgc
     7741 cattgaccta accgtggctc gatgattttg tcggttatca ggccgccaac aatcgtcagt
     7801 acgactacgg agctggccat aaaataccag ttatcaatta cactgacgtg catttgcgga
     7861 ttgaacgcag ctgccgcttc cgtgctgatc cccgacagca acacgtcggt tgtgacaatc
     7921 agtaaattag ccgtaaagcc gcaacctacg cctgcaatgg cagccagtaa acctgcaact
     7981 ggatgcctgc ccaccgccag aaaaatcagc gcacccatcg gtggcatgat cactaacgcc
     8041 gcatcggaag aaatgtggct gaaaaaagca ataaacagca ccatataact ggcgtagcgg
     8101 gcattaacat gcgatgccat tttaaccatt agtgctggca gtaagccgac gcgctccgcc
     8161 agaccggcac ctaaaaccag cgccaggatc gcaccaagtg gagcaaaacc gctaaagttt
     8221 ttaataacat tgggtaaaaa ccagtgtaat ccttccacac tgagcaggtt tttcaccacc
     8281 accggcgtac catcggtcgg gtttttcgca ctgacgccaa aggccgacaa aattgccgtc
     8341 gtcaccatga gtacgataat caaatagata aagagcaaaa aaggatgggg aaccttgtta
     8401 ccaattcttt cgacccagcc atagagcttc ccggattggg aggacgacgg tatggatgac
     8461 atactcatgg gcattcctcg gttgttgtgt ttgcgttgtt gttgttattt taaaggtgac
     8521 ggtgtcacgt ttttcgggat agggcagtgg tacggttgcg tgtccgttac ttgctgatgc
     8581 tcttgttggc attcttgcaa tagccctgaa tcaaggaaga gattgactgt ggttgctgcc
     8641 atagttttcg ccgccagcag cattccttta tgagcaatag atgttcgccc ctggctaacc
     8701 agttgccacg tatgtagcgg tgtaccgacg gcaaaacagg ggctgaaaca ctgggcaaca
     8761 ggcagtttcc aactgacgtc gccgacatca gtcgatgccg caagcacgtt atcggtggcg
     8821 gcatatggag cgacttcatt cgccagtacc gtttcacgat gacgtagtgc aaaaaccttg
     8881 ccgttttcgc caccggttgc ggcgatatta ttcagactgt tttgccgatc gttggaggtg
     8941 agcgtagcct gaatttgttt cgcaaaagcc agttcttcgg agttccattc cggggtacca
     9001 aaatgggata gggcctggta catggcattt tctaaggtgc gattcgggag ataactggaa
     9061 caggctttgt cgaagcggca ttcaaccgtg gtttcggtca tcaatgccgc accttcggcg
     9121 attttggcga ctcgatcata aatatgctgt acgtcggtca tttcgggggc gcggataaga
     9181 taaagcactt ctgcctgcgc ctggaccacg ttgggcgaga tcccgccgct atttgtgatg
     9241 gcatagtgta cgcgcgcttt ttcaataata tgttcgttga ggaagttggt gccagtggtc
     9301 atcaacgtta cggcatcaag ggcgctgcgt cccaaatgag gggaattcgc ggcatgtgct
     9361 gcgatccctt taaagcgcca tgatgcctga atgtttgcca gcgtgcgggt attgaacata
     9421 ccggcaaagg cttccgggtg ccaggtgagt gccgcatcca catcatcaaa taccccctcg
     9481 cgaaccatga acgttttacc cgagccgcct tcttcgccag gacaaccata aaagcgcacc
     9541 gtgccgcctt gcccatattg ttccagccat ttcttgacgg ctattgcagc ggcaaaggcg
     9601 gcggttccca gcaaattgtg tccgcaaccg tgaccatttt caccgggcgt cacggatgta
     9661 ggttgcgcgc aacctgcttg ctgacttaaa cctgccaggg cgtcatattc tcccagcagg
     9721 gcgataaccg gtttgccttg accaaacgaa gcaataaagg catttgggat attgcctacg
     9781 ttgcgggtaa cggtgaagcc tgcagattcc agcgccgaag ccagatgctc cgctgaccag
     9841 aactcttcaa aacgtgtttc tggatgatcc cagatttgat cggcaatatc ggtataacgt
     9901 tggcgatcgg cttcaatcgc atcgtcgata aaacgataga tttcctgcat cagatacctc
     9961 gcgtccaggg aaaattgagc gcggtgcgcg ccagcgtttc gacggcaata gcgagaacct
    10021 gctcgtcaaa atcgaatttt tcgttgtgat gacctgccgc cagctgtgtg ccaaacacca
    10081 tgtaggaggc ttgcccttga tgttgctgca cgcgggccat cattaatgtg gcatcttcgg
    10141 aacccgcagg cgcttcgaca cgttcaatgg cctgattgac ccccgcgacc tgagccgcct
    10201 gactttgcaa ccatgcgacc cattgcggcg aaggagaact ggcggtagct gcacccatca
    10261 gacgaatttc aacgccgaca ccatacatgg ttgctgcgcc ctgaatcgct tgttgtgcac
    10321 ggtcaaaaac atattgatta atgacgtcgc tggccccgcg tgtttccact ttcagcaacg
    10381 ccgaggcagg aacaacatta cgaccgcttc ctgcctgcat aacgcccacg tttactctgg
    10441 aagcgccttc gctgtgcggg gcgattgcat gcagtgcaag agtggcttgt gctgccgcca
    10501 acaaggcatt gtgaccgtct tctggttttg ctcctgcgtg agcggcggtc ccggtgaagt
    10561 gcgcgtcaaa tttggtggtt gccataaaat tatcactgcc gcacaccacg gtgcccgcag
    10621 gtacgccagt gccaatgtgc acggcagtaa aataatcaac atcatccact acacctgcat
    10681 cgaccatcgc ccgcgcgcca cgcgtacctt cttctgcagg ttgaaaaatc agtttgatga
    10741 tgccatgtag tccggactcg aactgtttaa gggtatgcgc cagcccaagc ccaatggcgg
    10801 tatgcccatc atgaccacag gcatgcatca ttccggcgtt acatgacgca aaaccgtcgc
    10861 ggtaggggcg atggctgaca tcctgctctt cactgagatc cagcgcgtcc atatcgacac
    10921 ggaaagccat caccggaccg gggcgaccgg tatccagggt ggcgacgata ccagtgaaac
    10981 caccttcaaa agccgcaatc cattgtgcta gcgcaccctg ttgccgagcg cgctcgaatt
    11041 cgcgttgtag agtgagttca tcaggtagtc ccatccggct actttcatta actacttcgc
    11101 ggcccagcgc cagtgaatag ccgagctgct gcaattcttc cgcaacaagg gtggcagtgc
    11161 ggaattccac ccagccagac tctgcatagt gatgaaaatc acgtcgccag tgcgataatt
    11221 ttggggcaag cgaattaaca aattgattca aagactccat aacctttccc gtcatcagta
    11281 aaaagtgtga cccggttcac gtagcgatag tttttactta tcactaacag atttttcaca
    11341 gttttaaccg ttcataaatt accctgacac aatcatctgc attaaagtag atgccagttt
    11401 ctttggtctg ataaataacg gttatcggtg gcgtcatggc ttttcaggta aaaattcatc
    11461 aaattcgggc ttttgttgaa gtggctcgtc agggcagcat tcgcggagcg agccgaatgt
    11521 tgaatatgtc gcaaccggca ctgagtaaat ctattcagga gctggaagaa gggttagcgg
    11581 cgcaactctt ttttcgccgt agtaaaggcg tgacgttaac tgatgccggt gaaagttttt
    11641 atcagcacgc cagtcttatt cttgaagagc tgcgcgcagc ccaagaggat attcgccaac
    11701 gacaagggca actggcaggg cagattaata tcggcatggg ggccagtatt tcccgcagtc
    11761 tgatgccagc tgtcatatct cgttttcatc agcagcatcc gcaggtaaaa gtacgcatta
    11821 tggaagggca actggtgtcg atgattaatg aattgcgtca gggagaattg gatttcacca
    11881 tcaataccta ttatcaggga ccgtacgacc acgaatttac ttttgagaaa ttactggaaa
    11941 agcaattcgc gatcttttgc cgcccgggac accccgccat tggtgcccgt tcgatcaaac
    12001 agttactgga ttacagctgg acaatgccga cgccacacgg cagctactac aaacagttga
    12061 gtgaattgct tgacgatcag gcgcaaacgc cacaggtcgg tgtagtctgc gagacgttct
    12121 cagcctgtat cagtctggtg gcaaaaagcg attttctcag catactgcct gaagaaatgg
    12181 gctgcgatcc cttgcacgga caggggctgg tgatgttgcc ggttagcgaa attttaccga
    12241 aagcggccta ttatttgatt cagcggcgtg atagtcgcca gacaccactg accgcgtcat
    12301 taatcacgca attccggcga gaatgcggct atctgcaaag ttaaaactgc ataaaaaaat
    12361 agagtctgtc gacatccgcc agactctata gtacacacag cagtgcatcc gcgtcttaaa
    12421 tccggcattg tctcctctgc gccggtgact gtgttataac ggttttaacc tttagttgcc
    12481 aattttcttc agtgacagat ttcacgaaaa tataagttac attattaata tcgtgaatga
    12541 ataatcatgc ataagcattt tgcttaaaat atcggcaaca tttggaactt attactggaa
    12601 ttttgggtaa tacgttgttg gaccgacccg gtctggttat catatcgcgc tcttaattgc
    12661 gggaggatgt aacatgaacc ttgacgacaa atcgctgttt cttgacgcca tggaagatgt
    12721 ccagccgctg aaacgtgcta ccgatgtcca ctggcatccc acgcgtaacc aacgtgcgcc
    12781 gcagcgtatc gacacgctgc agcttgataa tttcctcacc accggatttc tcgacatcat
    12841 cccgctaagt cagccgctgg agtttcggcg ggaagggttg caacatgggg tgctggataa
    12901 gctgcgcagt ggtaaatatc cgcaacaggc gagcctgaat cttttgcgcc agccggtgga
    12961 agagtgccgc aaaatggtgt tcagtttcat tcaacaagcc ctggcggatg gtttgcgtaa
    13021 cgtgctgatt attcatggta aagggcggga tgataaatcg catgccaata ttgtccgcag
    13081 ctatgtggcg cgctggctga ccgaatttga tgatgttcag gcatattgca ccgcgctacc
    13141 gcatcatggc ggcagcgggg cgtgttacgt cgcactacgt aaaacggcgc aggcgaagca
    13201 agaaaactgg gagcgccacg ctaagcgcag tcgttgatct cgagacgcat ccgcggctta
    13261 tgccgccagc acgcggttgc gtccatcatt tttcgcccga tacaaagcat catcaacgcg
    13321 tttaaacagt tcatcgatgc tttcatttcc ttcgtgatgc gccacaccaa tgctgacggt
    13381 aaagcgtggt aagcccgaaa tactcacttt tgccacgctt acgcggatag tttcagccag
    13441 cgaaagcgcg gtatccagtg gggttcttgg tagcaataag acaaactctt cgcctcccca
    13501 acgaaacacc aaatcgcctt tgcgagcgca actttcgagg gtgcgggcga gggcgcataa
    13561 cacctcatca cctttagaat gcccatagag atcgttaatg tgtttaaaac gatcggtgtc
    13621 gatgagcaac aagctgtaat cctgagcgat ggcgagatgc tgcatttggc ctggttccgt
    13681 aatgtgataa aactgtcgcc gattcagtaa tccggtcatc gcgtcatggt gagcagcatg
    13741 ttccagctgc tcctccagcc gtttttgctc agtaatatca tgcacaatac ataacatgag
    13801 cttgtcgcca taaatttcaa tcggtccggc ataggtctgc acatgacgag tagatccatc
    13861 cgccagttta tgaacaaaat tcaaaggttt atgaccgccg ggtaaatgcg aaatttcatg
    13921 catgataggc atgacgcgac gcccgagcat atttatttcc caggtatgtt tttggcacat
    13981 cgtttcatgg ttataaccat agaaattgag cgcggcgagg ttagcatcga cgatttggcc
    14041 atctcgtgac gggtcaatca acaacattgg tgcagagtta gtcagaaaaa agcgcgcata
    14101 aaaaccttgt tttttgcgct gataatttgc cgagcgactg gcttttaaac ccagcgttgc
    14161 cggcgcttcg ataccttcga aaataatcac cggttctgtt tctgtcagct ctcgcaaaac
    14221 aagccgacag cttaatgctg tttcctcttc tttacgctga acagtgagga tttcgataat
    14281 atcgtgctgg ttttgcagat cggagaggta tttcggcagt tctttttgtg aggagacgga
    14341 atagggaccg gttcgtagct gactaaacgt gaggtcttgc atcaacagtc gcgccgcgct
    14401 gttggcataa attaactgtt cctcaaaggg cgaaacgatc cagacaggac tggtgagtaa
    14461 gtccagggta ttgaagttgt gcgtaatcat tgagatcccg ttatttttat caatttttgt
    14521 tgctatccga tcgcaaaaaa gccacatcat atgatcagat aattctgata atgatagacg
    14581 ctatttaaca cttcacacgg tttgtatacg gaaaagcatt ttgctttttg tattcaattt
    14641 agacagaatt ttattaatca tttcagggta atggggtgat gagatgttgc gtaacagggc
    14701 cagaaggcta gactacaaaa taatgcgttg atgatggagg cactgtggaa gcgattaagg
    14761 gatcggacgt taatgtcccg gatgcagtat ttgcctggat gctggatggt agaggcggcg
    14821 ttaaaccgct ggaaaataca gatgtgattg atgaagcgca tccctgctgg ctccacctta
    14881 attatgtaca ccatgacagc gcccaatggc tggcgacaac accgctgctt cccaataacg
    14941 tacgtgatgc gctggcgggc gagagcacac ggccccgagt cagccgtctc ggtgaaggca
    15001 cgctgattac attgcgctgt ataaacggca gcaccgatga acgccccgat caactggtcg
    15061 ccatgcgtgt atatatggac gggcggttaa ttgtttcgac ccgacaacgc aaagtgctgg
    15121 cgctggacga tgtggtgagc gatctggaag agggcacggg tccgaccgat tgcggggggt
    15181 ggctggttga tgtgtgcgat gcgttgaccg atcattcaag cgaatttatc gagcagctgc
    15241 acgataaaat tatcgacctt gaagataatc tccttgatca gcaaattccg ccgcgtggat
    15301 tcctggctct gctgcgcaaa caattaattg tgatgcgtcg ctatatggca ccgcaacgtg
    15361 atgtctatgc tcgtcttgcc agtgaacgtt tgccgtggat gagcgatgac caacgccgtc
    15421 ggatgcagga tattgccgat cgccttgggc gcggccttga cgaaatcgac gcctgtatag
    15481 cacggactgg tgtgatggcg gatgaaatcg ctcaggtgat gcaggaaaat ttagctcgtc
    15541 gtacctatac aatgtcattg atggcaatgg tctttttacc cagtaccttt ctgacagggt
    15601 tatttggcgt caaccttggt gggatccctg gcggcgggtg gcaattcgga ttttcaattt
    15661 tttgtattct gttagttgtt cttattggtg gtgttgcttt atggttgcat cgtagtaaat
    15721 ggttgtaaca aaagcaattt ttccggctgt ctgtatacaa aaacgccgca aagtttgagc
    15781 gaagtcaata aactctctac ccattcaggg caatatctct cttgcaggtg aatgcaacgt
    15841 caagcgatgg gcgttgcgct ccatattgtc ttacttcctt ttttgaatta ctgcatagca
    15901 caattgattc gtacgacgcc gactttgatg agtcggcttt tttttgcctg ttatttatca
    15961 gcgtctaccc tttaagagtc cacccaatga ccggagggaa atatgacgac acttatttat
    16021 ttgcaaattc ctgtccctga accgattcct ggcgatcctg ttccagtgcc cgatccgatc
    16081 cctcgcccgc aacccatgcc tgacccacca cccgatgaag aaccgattaa attgtcgcat
    16141 cgtgagcgta gatctgcgag gatacgcgcc tgatgacttt gcgtcgatga ccacgagaat
    16201 agattgtgac cgctttttct accctgaatg ttttgcctcc cgcccaactc acgaacctta
    16261 atgagttggg ttatttaacc atgacgccgg tgcaggccgc cgcgcttccg gcgatccttg
    16321 ccggaaaaga tgttcgcgtg caggcgaaaa ccggcagcgg caaaacggcg gcttttggcc
    16381 tcggcttgtt acagcaaatt gatgcgtcgc tatttcaaac ccaggcttta gtgctgtgtc
    16441 ctacgcgtga actggcggat caggtggcag gtgaattgcg tcggctggcg cgttttctgc
    16501 caaataccaa aattttgacg ttgtgcggtg gtcaaccgtt cggtatgcag cgtgattcgt
    16561 tgcaacatgc gccgcatatt atcgtggcaa cgccgggtcg tttgctggat cacttgcaaa
    16621 aaggcacggt atcactggat gcgctgaata ctttggtgat ggatgaggcc gaccgcatgc
    16681 tggatatggg atttagcgat gccattgatg atgtcatccg ttttgcgcct gcatctcgac
    16741 agacgcttct gttttcggca acctggccgg aagccatcgc tgcaatcagc ggacgagtgc
    16801 aacgcgatcc tttggcgatt gaaattgact caacagatgc tttgccaccc attgatcaac
    16861 aattttatga gacatccagt aaaggcaaaa ttcctctgtt gcaacggtta ttaagcttgc
    16921 atcagccatc ctcttgcgtg gtgttttgca ataccaaaaa agattgccag gctgtctgcg
    16981 acgcgctgaa tgaagtaggg caaagtgcat tgtcattaca cggcgatttg gagcaacgcg
    17041 atcgtgatca gaccctggta cgttttgcta acggtagcgc ccgtgtactg gtcgcgactg
    17101 atgttgctgc gcgtggtctg gatattaaat cgcttgagct ggtggtgaac tttgagctgg
    17161 cgtgggaccc tgaagttcat gtacatcgca tcggtcgtac agctcgtgca ggaaatagcg
    17221 gtctggcgat cagtttctgt gctccggaag aagcacagcg ggccaatatc atttctgaca
    17281 tgttgcagat aaaacttaac tggcaaacgc cgccagctaa tagttccatt gtgacgctgg
    17341 aagcagaaat ggcaacgttg tgtatcgatg gcgggaaaaa agccaaaatg cgcccgggtg
    17401 atgtattagg tgcactgaca ggagatatcg ggcttgatgg cgcagatata ggcaaaatcg
    17461 ccgtgcatcc ggcgcatgtc tatgtcgcgg tccgtcaggc tgttgctcat aaagcatgga
    17521 aacagttaca gggcgggaag attaaaggaa aaacgtgccg ggtgcggtta ttaaaataat
    17581 gaaatgttga attgccgggt gcaagagtaa acatcttatt cgggattgcc ggatgcgacg
    17641 ctggccgcgt cttatccggc ctccataaga gtagcccgat acgcttgcgc atcgggcgct
    17701 atcttggtta tttcacttca accacattca gccgtaactc atccaactga ttttcatctt
    17761 cttctggctg ccagcccgcc ggttgtagtg ggatctcttc gcgatcaaac gccagatcac
    17821 ccccgttaac cacttcagaa ccgtgggtga tgcctttgaa atcgaacagg ttggtatcgc
    17881 acagatgcga tggcaccaca ttctgcatcg cgctgaacat cgtctcgata cgccccggat
    17941 aacgtttatc ccagtctcgc aacatgtcag caatcacctg acgttgcagg ttaggctgtg
    18001 aaccgcacag gttgcacgga ataatcggga acgcttttgc atcggcaaat cgctgaatat
    18061 ctttctcgcg gcagtaggcc agcggacgaa taacgatatg tttgccatca tcgctcatca
    18121 gtttcggagg catacctttc atcttgccgc cgtagaacat atttaagaac aacgtttgca
    18181 ggatatcgtc acgatggtgg cccaacgcga tcttcgtcgc ccccagttcc gttgcggtgc
    18241 gataaaggat accgcgacga agtcgagaac acagtgagca agtggttttg ccctctggaa
    18301 tcttctcttt cacgataccg taagtgttct cttcaacaat cttgtactca acgcccagct
    18361 tttcaagata ctcgggcaga acgtgttccg ggaagcccgg ttgcttttga tcgaggttaa
    18421 cagccaccag cgaaaaattg attggcgcgc tttgctgcaa attgcgcaga atctccagca
    18481 tggtatagct gtctttaccc ccggagaggc aaaccatgat gcgatcgcct tcttcaatca
    18541 tattgaagtc agcaatggct tcgcccacgt tacgacgcag gcgtttttgt aatttgttca
    18601 gattgtattg ttctttcttg ctaatttctt gattttgcga catttaaaag cgactcaatt
    18661 cgttatatgg catcagaaga gtatgcgtca tgccggaacg cccagcataa gaaatctgat
    18721 ataaaaactg tggcgtgtat ggtacggatt agaggggaaa atgtcagcac atttgcgaaa
    18781 tgaatcaaaa agcccgcagc aatgtgcggg cgttagtgtc agcgcacaac cagcacggag
    18841 cactctgcgt gacgcacgac agctgcggcg ttagaaccga gcagataagt ggtgatatcc
    18901 ggtcgatggg aagcaatgat gatcatatga gcggggatct tcttcgccaa ttccagaatg
    18961 cggtctttgg gcgaaccttc ctcaacatgg acatgcactc tgtcggttgg cagtttaaat
    19021 tttttaatga tctcttccag ttgcgatttg gcttccgctt tcaggtcatc cattgccggt
    19081 aattctgcgg aatacgctaa acccagagag gcatagtagg gcagtgaagg tattaccgtc
    19141 aggaaatgaa cctctgcatc atcaatcttt gcctctgcct caacgtggct aatcacgcgt
    19201 tgagttaatt ctgaatcgga aatatcgata gggacaagaa tcgttctgtt cataaaacct
    19261 cctgttttag tatccgcata aagtgtaacg ccagatgaca ctttttgtgt aatgacggag
    19321 ttcacatttt taatttagat caaaggagga agaataagca gaaaaagccc gccataacag
    19381 cgggcaggag gatttagaac tgataaacca gacctaaagc gacaatatca tcggtagaga
    19441 tgccgttggc agcgtagaaa ctgtcatctt catccaacag gttgatttta tagtcaacat
    19501 aagtggacat gtttttattg aaatagtaag tcgcgccaac atcggcgtat ttaaccagat
    19561 ctttatcatc aacacctgcc gggttgtctg caccaccggc agcgtgcagg tcacggcctt
    19621 tagacatcag gaaagagact gccggacgca gaccaaaatc aaactggtac tgtgcagtga
    19681 cttcaaagtt ctgggttttg tttgcaacag cataatcgct gtcgccaaac ggggtcatat
    19741 tacgcgtttc tgaatacatg gttgccaggt aaatattgtt agcatcgtat tttagcccag
    19801 cagtccacgc gtctgcttta tcaccacccg ccgcagtatg gttaacctgg tcattggtgc
    19861 ggtcagaaga ggtgtatgcc gcaccagcgc taaagcccat gcctaaatca tatgttgtgg
    19921 aaagacccca gccgtcaccg ttttcatggc gaacatcacg tccgttgttg gtgccttcct
    19981 gaccattact ggctccttcg ttattacctt gatactgcac cgcgaagttc agaccattta
    20041 ccagaccgaa gaaatcagta ttacgataag tcgcgacgcc attggcacga ccagtcataa
    20101 agttgtctgc attggtataa gagtcaccgc caaattcagg cagcatatcg gtccagcctt
    20161 cgatgtcgta cattacgcca taattacgtc cgtaatcgaa agaaccgtaa tctgcaaatt
    20221 tcagtccggc aaatgccaga cgggtccatg actggttttt tgaagattca gtgttgtttg
    20281 cctgaatatt gtattcccat tgaccgtagc cagtgagttg atcgttaatt tgggtttcgc
    20341 ctttaaaacc cagacgcgca tagctctggt cgccatcttt cgctgaatta tcagaaaaat
    20401 aatgcaggcc atcaactttg ccatacagat ctaatttgtt gccgtcttta ttataaactt
    20461 cggctgcatg tgcagcacct gcggcgagca gggcaggaat taaaagtgcc agtactttgc
    20521 ttttcattga ataaatcctt tagttatttt atttgccttt tatccccatt tcggggagtg
    20581 aaaacatcct aaaggagaag ttcagttagc cgataaatat catttgttac gccttaagta
    20641 aaac
//
LOCUS       DAPGRN010000086        20218 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPGRN010000086 DAPGRN010000000
VERSION     DAPGRN010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.086"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(100..846)
                     /gene="cutC"
                     /locus_tag="RHW33_003285"
     CDS             complement(100..846)
                     /gene="cutC"
                     /locus_tag="RHW33_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV1646761.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANSLNTLNNLAELGIVRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(860..1426)
                     /locus_tag="RHW33_003286"
     CDS             complement(860..1426)
                     /locus_tag="RHW33_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1646762.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1642..3375
                     /gene="argS"
                     /locus_tag="RHW33_003287"
     CDS             1642..3375
                     /gene="argS"
                     /locus_tag="RHW33_003287"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV1646763.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3552..4040
                     /locus_tag="RHW33_003288"
     CDS             3552..4040
                     /locus_tag="RHW33_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV1646764.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4162..4554)
                     /gene="flhe"
                     /locus_tag="RHW33_003289"
     CDS             complement(4162..4554)
                     /gene="flhe"
                     /locus_tag="RHW33_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV1646765.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4554..6632)
                     /gene="flhA"
                     /locus_tag="RHW33_003290"
     CDS             complement(4554..6632)
                     /gene="flhA"
                     /locus_tag="RHW33_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV1646766.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNAVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATIDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6625..7773)
                     /gene="flhB"
                     /locus_tag="RHW33_003291"
     CDS             complement(6625..7773)
                     /gene="flhB"
                     /locus_tag="RHW33_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV1646767.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7975..8619)
                     /gene="cheZ"
                     /locus_tag="RHW33_003292"
     CDS             complement(7975..8619)
                     /gene="cheZ"
                     /locus_tag="RHW33_003292"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV1646768.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8630..9019)
                     /gene="cheY"
                     /locus_tag="RHW33_003293"
     CDS             complement(8630..9019)
                     /gene="cheY"
                     /locus_tag="RHW33_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV1646769.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9034..10083)
                     /gene="cheB"
                     /locus_tag="RHW33_003294"
     CDS             complement(9034..10083)
                     /gene="cheB"
                     /locus_tag="RHW33_003294"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV1646770.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKVSLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGLAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10086..10946)
                     /gene="cheR"
                     /locus_tag="RHW33_003295"
     CDS             complement(10086..10946)
                     /gene="cheR"
                     /locus_tag="RHW33_003295"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV1646771.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10965..12566)
                     /gene="tap"
                     /locus_tag="RHW33_003296"
     CDS             complement(10965..12566)
                     /gene="tap"
                     /locus_tag="RHW33_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV1646772.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRNDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12612..14273)
                     /gene="tar"
                     /locus_tag="RHW33_003297"
     CDS             complement(12612..14273)
                     /gene="tar"
                     /locus_tag="RHW33_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HDV1646773.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14416..14919)
                     /gene="cheW"
                     /locus_tag="RHW33_003298"
     CDS             complement(14416..14919)
                     /gene="cheW"
                     /locus_tag="RHW33_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV1646774.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14940..16904)
                     /gene="cheA"
                     /locus_tag="RHW33_003299"
     CDS             complement(14940..16904)
                     /gene="cheA"
                     /locus_tag="RHW33_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV1646775.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGEPPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16909..17835)
                     /gene="motB"
                     /locus_tag="RHW33_003300"
     CDS             complement(16909..17835)
                     /gene="motB"
                     /locus_tag="RHW33_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV1646776.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17832..18719)
                     /gene="motA"
                     /locus_tag="RHW33_003301"
     CDS             complement(17832..18719)
                     /gene="motA"
                     /locus_tag="RHW33_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV1646777.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18846..19424)
                     /gene="flhC"
                     /locus_tag="RHW33_003302"
     CDS             complement(18846..19424)
                     /gene="flhC"
                     /locus_tag="RHW33_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV1646778.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19427..19777)
                     /gene="flhD"
                     /locus_tag="RHW33_003303"
     CDS             complement(19427..19777)
                     /gene="flhD"
                     /locus_tag="RHW33_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV1646779.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4848 a         5569 c         4933 g         4868 t
ORIGIN      
        1 tttttatact attcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga
       61 gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc
      121 aatgattcct ttcatttcag caaccgccgc cccgtctacg acataacgcg aatactcgtc
      181 cgcgtgtgca tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg
      241 ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt
      301 ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg caataagttc
      361 cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gcacccggac
      421 aatgcccaat tccgcaagat tattgagtgt atttagagag ttagcgcaca tatcaaaggc
      481 gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca tccgtggcat
      541 atcgacattc ccgtcaacat cgagcacgcc cgttaccagt ccaggaaaac ctaattcgcg
      601 gactgtgcgc acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc
      661 accgcgtggg cggataatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag
      721 tacacccaac gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc
      781 tgcgccgttt tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa tttccagtaa
      841 tgccattttt actccttaat tacgccgact gctcgctggc aacgatctct tcaatggacc
      901 acggatgaaa cttaatagtt gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc
      961 gctcatgctc accttttggc gaactggttt taactgagat ccccggtagg ctgagtcctt
     1021 catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc ggcagaacga
     1081 tttcaatatg ttcccaacct tcgtgcgggt aacgtttttc cccgggccac ggtagctcca
     1141 caatggaaaa ctgccagtga gcaacctgta ccggttcatg caatttaaac agacaaatcg
     1201 gtctgccatt gatcatattt tctgacaaaa gctcgccgca ctgttcaaac ccgcgacgcc
     1261 agcgttcagc ggtggcgttt tgatggcaac gcaaggaaat gtgatcggca gtcaacggag
     1321 tgatatccaa ccccaaacgg cgggaaagtt catctaatgc gtgggtaaat cgcggtaaat
     1381 ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc
     1441 tttcagtaaa agcgttaatt taccctgttg ccctgcgcca accaaccgct gatttcacgc
     1501 cgcttctgat gcaatagtga aaacggcaat acggcacgcg cacgttgctg acgaaaacag
     1561 ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct
     1621 tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag
     1681 gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca gtcagcaaaa
     1741 gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg
     1801 gcaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc
     1861 aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct
     1921 gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc agaaaaacag
     1981 accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcacgt cggtcacctg
     2041 cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg
     2101 attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgttgat tgcatggctg
     2161 gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg ctgaccttga aggtttctac
     2221 cgcgatgcga aaaagcatta cgacgaagat gaagagtttg ccgagcgtgc acgtaactac
     2281 gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa actggtcgac
     2341 atcaccatga cgcagaacca gatcacctac gatcgtctca acgtgacgct gacccgtgat
     2401 gacgtgatgg gcgaaagcct ctacaaccca atgctgccag gaattgtggc ggatctcaaa
     2461 gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg tattccttga tgagtttaaa
     2521 aacaaggaag gcgaaccgat gggcgtgatc attcagaaga aagatggcgg ctatctctac
     2581 accaccactg atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc cgatcgcgtg
     2641 ctgtattaca tcgactcccg tcagcatcaa cacctgatgc aggcatgggc gatcgtccgt
     2701 aaagcaggct atgtaccgga atccgtaccg ctggaacacc acatgttcgg catgatgctg
     2761 ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact ggccgatctg
     2821 ctggatgaag ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc ggatatgcca
     2881 gccgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa atatgcggat
     2941 ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct ggcgtttgag
     3001 ggtaataccg cgccatacat gcagtatgca tacacgcgtg tattgtccgt gttccgtaaa
     3061 gcagaaattg acgaagagca actggctgca gctccggtta tcatccgtga agatcgtgaa
     3121 gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt tgcccgtgaa
     3181 ggcacgccgc atgtaatgtg tgcttacctg tacgatctgg ccggtctgtt ctctggcttc
     3241 tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag ccgtctgaaa
     3301 ctggcacaac tgacagcgaa aacgctgaag ctgggtctgg atacgctggg tattgagact
     3361 gtagagcgta tgtaatcgat ttttcgtgag agtgaagcct gatcagggtt agccgatcag
     3421 gcttttttat tgccatctaa atgtattctg aaaacggaca tgccatcgtt ttctcactgt
     3481 tgagtaagag gccagaagcg taatatccag gcccaggaaa acgataacgg ttcaatttaa
     3541 ggaataccgc agtgtttaaa tttcttgtat taacattagg cattatctct tgccaggctt
     3601 acgcagaaga tacggttata gtaaacgacc atgacatttc agccatcaaa gattgttggc
     3661 aaaaaaattc agatgatgat actgacatta acgtgatcaa atcatgcctg cgacaagaat
     3721 acaatctcgt cgatgcgcaa ttaaataaag cttatggtga agcttatcgt tatatagaac
     3781 aagtgccacg cacaggtgca aaaaaacctg atactgaaca acttaacttg cttaaaaaat
     3841 cacagcgagc ctggctggat tttagggaca aagaatgtga attaatcctt tcaaatgagg
     3901 atgttcagga tttaagtgac ccttattctg aatcagaatg gctatcatgt atgatcatac
     3961 agaccaatac gcgaactcgc cagttgcagc tataccgtaa ctctgaagat ttttatccaa
     4021 gccctttgac aagaggataa ttcacatctt tttggcatgt tttgttgcaa gctattcctg
     4081 ataaataatt gcaacaaaac atcgagcctt tttcactgag ttattaaaca tgctcgcgag
     4141 cgcgtaattt ttatttgtcc ttcagcgata attcacaatc acttcattcc gttgtacctt
     4201 tagcggtgga attaaccgcc caccgccagg cacttcccag ataaagcgca acggttctgc
     4261 tgccgctata ccggaaaagg ccacggtggt tccgctctgc ccctctaatt cgacacaacg
     4321 agattgcgaa cacaagcgaa cccgcagtcc tgcaggtgtc gggccgataa gctgataacg
     4381 ccacgccacc agcgtcatca atcctgaagc aggttgtcga gtagaaagag gcgctgacga
     4441 catcgactca ccgcgatgat ttagcgtaat tccgacacta cttgcctgcc acatcccctc
     4501 cccggcggct tgcactagca gcggaaacaa taatattgct aataaggttc tcatcattta
     4561 ccgccaattg tcgccgtcat gcggatatgt cggttatcag atagttccag attcgacagc
     4621 accactaact gcggcaagct gcggcgcaga aagcgagaca ataatggtcg cagcgcgtgg
     4681 ttcaccaaca atactggcgg cgcacccaac atctcctgac gggatagcgc ttcctgagtt
     4741 tgcgccagta aacgatccgc cagccctggc tccagtccgc ccccgccctg caacgcctgt
     4801 agcaacaaac gttccagcgg tgtatcgagg ccaataacat ggacttcatc tttgccagga
     4861 aaccactgct gggtaatcgc ccgtcccaac gccacgcgca cgacggcggt taattcatgt
     4921 ggatcacttt ggatgggtgc atgttccgcc agcgtttcga gaatggtgcg catatcgcga
     4981 atcggtactt tttcatcgag gagattttgc agcactttat gcaatgtggt gagcgtgacg
     5041 acgccaggaa cgagatcttc cgtcagcttt ggcatctcct gggcgacgcg atccagcagc
     5101 tgttgcgcct cctgacgacc aaacagctct gcggcatgct ggctaatgag gtggttaaga
     5161 tgcgttgcta ccaccgtgct ggcctcaacc acggtgtacc cctgaatctg cgcctgttct
     5221 tttagcgcac tttcaatcca gatagcattc aggccaaatg ccggatcgat ggtcgcctca
     5281 ccaggtaacg tcccggcagc ggttccagga ttaatcgcca accaacgccc cggataagca
     5341 tcaccactgc ctatctccac gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc
     5401 atattgtcgc gaatgtgcac cactggcggc agaaatccca tctcctgggc aaatttcttg
     5461 cgaatactgc gtatacggcc caacaactca ccatcctgct ggaaatcgac catcgggatc
     5521 agtcgataac ccacttccat tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct
     5581 tcgacaacgg cattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt
     5641 tcgcgtccgc gtatccacca ggccagcccc agcaatccag cagtgaacag caaaaatacc
     5701 aggttcggca ttccaggcac caggccgagc aaaccgagca cggcggcgct taacaacata
     5761 acgcttgggt tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg
     5821 acacgcgtaa caatgacccc cgcggcggta gaaatcacca gcgccgggat ttgtgccacc
     5881 agaccgtcgc caatggtcaa gagcgtataa ctttccgccg cgtgtcccat gctcatgcca
     5941 tgttgcagca cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg
     6001 gcgatggcat cgccgcggac aaacttactt gccccgtcca ttgagccgta aaaatcggct
     6061 tcctgagtca cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccggcgtta
     6121 aggtcggcgt caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc
     6181 acttctgcga tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg
     6241 acaaacacca cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttca
     6301 accaccttcc ctgccgccgc tgcgccggta tgcccttcca ttaaaatgat acgggttgaa
     6361 gccacgttaa gtgccagacg caacagcgtg gtaaacaaca gaatggtcgg aaacgcagca
     6421 aactcaagcg tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata
     6481 ttgaaggtaa acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag
     6541 atcaacagga tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg
     6601 ggcaggcgca gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag
     6661 ggcttccggc accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca
     6721 gcgtttcagt tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg
     6781 gatttgttga ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag
     6841 cgtcgggacg ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc
     6901 tttagcgacc actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata
     6961 gtgggtcggg ttattgacaa tgacatccgc tttcggcaca tcggccatca tccgacgccg
     7021 tgcggcagct cgctgcatct gacggatccg ccctttaaca tgggggtcgc cttcgctttg
     7081 tttgaactca tcacgaatat cctgccgcga cattcgcagc tttttcaggt ggctgaagat
     7141 ttggaaaaag acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca
     7201 tagccctacc aaatccatcg cattacccat ggcggtaatc ggagactcgg ccattaagcg
     7261 catcatctgc ggccagtgat gccagagaaa aaaccccgtc acgctgccaa ccaggatggt
     7321 tttcaaaatt gctttaagca actccgcgcc agtctgtgcc gagaacatcc gttttatgcc
     7381 cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc
     7441 tcccagcatg accggagaaa tgagcgccac cagcaccacg ccgctaatca gtggcagcag
     7501 cgccagcatg gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtcatt
     7561 gataatactg tgatcaaaat gcagcccagc ggagagcatg cccgacaatc gacgggccag
     7621 cgacacaccg ccaaaccaga taacactaac gcccaccagc aaaatcagca gtgaggtcag
     7681 ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt tctagtcggt gaggagtggg
     7741 ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca atcctggaaa gagtattaag
     7801 cgtaaatgat gccagagcgc aaagcgttca atggtttgag taaggggcaa aacaggcggg
     7861 atttagggct tttgctgcca cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat
     7921 cgccttatca gaccgcctga tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa
     7981 tccaagacta tccaacaaat cgtccacctg atcctgactg gctaccacac cggctttgct
     8041 ggtatcgacc tgaggtccat taagcaaact ctgattttca cgctttggac gcgactcctg
     8101 ttccgggatg ttttccaaca gcaccatcag caactggcgt tcgatctcct gaatgacatc
     8161 catcatccgc ttaatgacct gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc
     8221 cagcagttgc gcgttagtaa agctggtatg cgcgggtaca tctgccagaa attgtcgcgt
     8281 atctgttacc aattcacggg cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca
     8341 acgttgggtt aacgcttttg ctgatttctc catttgatcc tgatgcggtt gtgacgcctc
     8401 aacactgttc agcgcccgct ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg
     8461 atcgcgcgca tccgggatgg cttccgccgc ttcggcaatg gcctgatcca gccccagttc
     8521 ccgcaaactg tcgcgcagca tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc
     8581 tgaatgctcg tcagcaggtt tgattgatgg ttgcatcata gtcgcatcct cacatgccca
     8641 gtttctcaaa gattttgttg agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca
     8701 catagccact ggcccccgct tgcgccgcag caatgatgtt ctctttcttc gcttctgcag
     8761 tcaccattaa cactggcaat gccgacatcg cgccatccgc acgaatcgtt ttcagcaatt
     8821 ccaggccatc catgttgggc atgttccagt cggagataac aaatccataa ccgcctgcct
     8881 gcaacttatt gagagcgtcg acgccatctt ccgcttcctc aacattattg aatcccagct
     8941 ctttcagcag gttacgcact atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt
     9001 taagttcttt atccgccatt tcacactcct gatttaaata cgtatcgcct gtccggcact
     9061 aatttttgcc aacatttgct ggcttacctg gctaagatcg accacttcgc agacaccacc
     9121 catattgatg gcctcgcgcg gcatgccgaa caccacgcaa cttgcttcgt tttgcgccag
     9181 ggtccatgcc cccgcctgac gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc
     9241 ggtcaggatc accccaaccg cattacgccc cgcctgtttg gcgacagaat ggaacaacac
     9301 atctaccgaa ggccgatgac ggttaaccgc caggccatca tgaattttga tttggtaatt
     9361 tgcgccacta cgcgccagct ccatatgccg atcgcccggc gcaatatagg catgccccgg
     9421 caaaacacgt tccccgtctt cggcttcttt aaccccgatc tggcaaagct tattaagtct
     9481 gtcggcaaaa gagcgggtga aaccgggcgg catatgctgg gtaattaaca gtgccgggct
     9541 ggaaagcggc aacggttgca gtacgtgacg aattgcctca gttccacccg ttgaagcacc
     9601 aatcgcaatc agtttttcag aactcaacaa cggccctgcc ttcagcgttg tcggtgccga
     9661 caatggctta tgtgctgcaa ggctcacctt tgctgctgta cgcacctttt cagcaatcat
     9721 ttcgctgtac gccagcattc cttcgcgaat acccagttgc ggtttggtga caaaatctat
     9781 tgcccccagc tccagcgcgc gcagcgtgac ttctgatcct ttgccggtca gggaagaaac
     9841 catcacaacg ggcattggac gcaaacgcat taatttttcg aggaaatcca gtccgtccat
     9901 ccgcggcatt tcaacatcca gcgtcagcac atcgggattg aatttcttaa tcaagtcacg
     9961 cgcgaccagc ggatcaggcg cggtcgccac catttccatg tcgctatggc tgttgatgat
    10021 ttctgtcatg atctggcgca tcagtgccga atcatcgaca gataacaccc tgattttgct
    10081 catcgttaat ccttacttag cgcatacacc gtctgaccac gcagcgtgaa gcggcgctcg
    10141 aggtggctaa agttttcaga gtgacccgca aacagcaatc cgtcgggttt aaggagcgga
    10201 acaaagcggc gcaaaatctc ctgctgggta gtttgatcga agtagatcat gacgttacga
    10261 cagaagatcg catcaaacgg ccccggcacg gtgtactgtt tcgccagtag attcagcggg
    10321 gcaaaatcaa catagttcgc cagctcctga cgcacgcgta ccagcccttc atgcggtccc
    10381 gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg
    10441 cgatagatac cgcttctggc tttttccagc acttcggtgt cgatatcact ggcaaacact
    10501 ttccagcgtc cgggcgcggt gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc
    10561 tcttcgccgg tcgaagccgc cgcgctccat acgcgatact cgccagaacg gcgacgtgcg
    10621 tgatccgcga gcagagggaa atgatgtgcc tcacggaaaa atgccgtcag attcgtggtc
    10681 agcgaattga taaacgcctg ccactcaccg ctgtgctgat tagattccag caagttcaga
    10741 taatgaccga aatccgtcag tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc
    10801 atgtcgcgtt tatggtcagc cagaacgatc ccggctcgtt gatatatcaa ttgacttatc
    10861 cgccgaaaat gcgcgtcgga aagcgccagg cgctcggtca tctgtaacaa taaagacgtt
    10921 tgcccacagg gcagagatga agtcatagcg ccttctcaat cacttcagga taccactggc
    10981 gcaatttgta actgcgccga ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc
    11041 gccacacgcg acgaaagatg gtcggcctga ttcgccagtt gttccgttgc caccgccgct
    11101 tcttctacca gggaggcgtt ctgctgtgtc acctgatcca tctggctgac agcctgcgca
    11161 acctgctcaa tcccccgccg ttgttcttcc gaggccgaag caatttctcc cataatgtcg
    11221 ttcacgcggg tcaccgaact gacaatatcg gtcatggtcg cggcggcgtt attcaccagt
    11281 ttcgaaccct gctggacacg attgactgac tcttcgatca gccctttgat ctcttttgct
    11341 gcctgcgcgc tacggctggc aagattgcgc acttcacctg ccactaccgc aaaaccacgc
    11401 ccctgctctc cggcgcgagc cgcttccact gccgcattca gggccagaat attggtctgg
    11461 aaagcaattc cgtcgataac gctgataatg tcgccaattt tttgcgagct ggtggcgatc
    11521 tcctgcatgg tgtgagtcat ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg
    11581 gcattttttg ccagttccga cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg
    11641 gttaattgct ccatactggc ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga
    11701 cttgagagat cgttattgcc tgcgacaatc tccgcgatcc caatgtgcat ctcctggctt
    11761 cccttacgca catcactcac cgtcccacgc aaagcctgct gcatggtctt caggctggca
    11821 aaaatggcgg tgatctcatt acgaccatat accgcaatcg gacgcgccag attacccgca
    11881 gcaatgctgt caaaatggct accgataatg gccagtggtt gaacaatcat cttgcgcgtc
    11941 caccacagcg cactgctgat gtagatcgcc gcaacaataa tcatgctgat aaacaccagc
    12001 gccgaaatct gatagttacg ctggctttgc gcactggcgg cttccagcac atggttgatc
    12061 tccagctgcc aggcctcaaa gttaacgtca aacgcattct gtgatccctg caccggcgca
    12121 gtgaggaaat ccgaaagttg attactttca agccaggtcg cctgatgttc gagatcgttg
    12181 tgccagcggg caaaactttt ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc
    12241 gcagtcatcg ccataaaact tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc
    12301 gtcgtcatca acgttttaat gtcatccgcc ggatagctaa gcgctgtcag agtgcctgct
    12361 ttgttcagcg cggtactggc ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt
    12421 tgctgattgc tctgctcgac ctgattcagt cgttgcaaat cgttgcgaaa tgcccaaaaa
    12481 gacatgccgt tactgccaat ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg
    12541 gtcgaaattc gaatacgatt aaacatcaac gctccccatc aggcggcaat gaccgcgtta
    12601 gtaaatactc gtcaaaatgt ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc
    12661 tgtgccggtg gttgctcact agcaggacgg gatggtgttt gcggtttatt ggtgagtggg
    12721 ctggctgcca gacgaaacgc ggaaaccgct tgcgttaaac gacttgcctg ttcttccagc
    12781 gcagctgcgg cggcagctga ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc
    12841 atttccgaaa ccgccaatgc gacttgatcg atgccacggc tctgttcatc cgatgccgat
    12901 gcaatctcgc ccataatgtc agtcacgcga gtgacagcat tgacgatatt gttcattgtt
    12961 tccccggcgc tttcgaccag caccgaaccg gtatcaacgc gcgagacgga gtcttcaatg
    13021 agagctttga tctcttttgc cgcctgggcg ctacgactgg caagattacg cacttcgccc
    13081 gccaccacgg caaaaccacg gccctgttca cccgctcgcg cggcttcaac cgcggcattc
    13141 agcgcgagga tattagtctg gaaggcaata ccgtcgataa cgctgataat gtcggcaatt
    13201 ttcttcgaac tatcggcgat ctcatgcatc gttttcacta cgccatccac cactttgccg
    13261 ccgtgctggg cggtgtcgga ggcactttgc gccagttgcg aggcctggcg ggcgttatcg
    13321 gcattttgct tcactgtcgc ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg
    13381 gatgcctgct gttcagtacg ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta
    13441 ccggcataga tggcatctga accttcgcgg acatgagtga ctgtgtcagt caaagagcgt
    13501 tgcatatgtg aaacgctctg cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg
    13561 gtattcgcca ggttaccacc ggcgatttcg cgaatatgag caataatttt tgccagcggt
    13621 gtaagcaaca tacggcgaat gccataccac gccaccagca gaatcaatac caccaccagc
    13681 gcgataaccg ccagttgcca ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata
    13741 tcgcgataca gtttttcact gctgagggcg tactgagcaa acgcttcgcc cattgcattt
    13801 tgcattccct gggttggctg agcgaaataa gctccagtat tgccataatc aagataatca
    13861 atcagttcag ttaacgctgt gtaatagttt ttatattttt catcaatatt acgactggta
    13921 gcgaccattt caggtaacgg tgccatgctt ttgaattttt tataatgagt cgcggcctgg
    13981 gccaatgttt tcctggcgct atcgagcaat tcaactttgg cattactttg ttgattggag
    14041 gaatccatca tcattcgcac cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa
    14101 tcccaggttg acgtcagctc gccctgctgt tcccgtaatt gattggaaac cacaaagctc
    14161 ttctggctat ggtgaaggga agaaaaaaac agactgccgg aaataagctg taacagtgcg
    14221 aataccccca gcaccattac caacagcgtg actacgcgga tacggttaat cataaggcac
    14281 cttcctgaaa acaagttgat ctcgttatcg gcaaggaagg gggaaacttt attgctgatg
    14341 ccacccgccg cgaaattgaa ataaaacccg atgcgcagat catcgggttc atttcaattg
    14401 aggaaatcag gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc
    14461 gctgttcagc agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag
    14521 tccagtgaga tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc
    14581 cgccgtcaat gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc
    14641 gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt
    14701 aattcgtaag tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa
    14761 cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag
    14821 gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg
    14881 ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt
    14941 caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca
    15001 acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg
    15061 cgatagttac tttcaaggtt tttaactaca acctgatgtt gaccaattaa ttgatccacc
    15121 agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg ggtagcttcg
    15181 gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc
    15241 cgcacttcca gcacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc
    15301 agtgattcca taacagcatt cagcggcaga atgaaaactt catccgcaac gcgtacggac
    15361 atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc
    15421 tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc
    15481 atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa gcctggtgca
    15541 aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag
    15601 gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg
    15661 caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct
    15721 gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg
    15781 gtcagcgggt cgataatgcg ttctatcagg ctcttgtcga gctcggtgga actgcccacc
    15841 agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag
    15901 cgactaaaga catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca
    15961 cgggcgttac gttgtaactg ccccatgctg gtaatcaaat caccatgatt aaccgggtcc
    16021 agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta
    16081 attaattgat caaccttttc taccgctaca cggatgctgg tggattcact gctgcgcgtc
    16141 gttttttccc gctccacgcg accggttggc gcttgttcgg ctgccagttt aagcactggt
    16201 ggggtggata tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc
    16261 acaaaacaga gtaccgctgt gatgtcatct tcggcgatat cgcccggtaa tattgccgag
    16321 agagaatccg cccctttcac cacgtcagtt aacgttgtca gatgccccag ctcttcttcc
    16381 agcaggtcga cttcgccggc cttcaggcgc gaaaggataa ttcgtcgcgg caactgactg
    16441 cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat
    16501 ggcggttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag
    16561 ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc
    16621 ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct
    16681 tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt
    16741 cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct
    16801 ggcgcttccg gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct
    16861 tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc
    16921 ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct
    16981 tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt
    17041 cagaaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg
    17101 catcgttgcc gccatgccga cgacacgtaa tactttgccg ctatccaacc cgccgaccat
    17161 cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt
    17221 ctcaccgctg gcgtagggaa aatcatcggt atgacctgaa aggctaatac ggttgggaat
    17281 accgttcagt acaggcgcga tggcgcgcag aatgtcgcgc atatagggtt cgacatcggc
    17341 actgccggtt ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc
    17401 ctggaccaga tcgattttga gatgtggacg taacgcccgc agtttcggat cggactctat
    17461 caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcaa
    17521 ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat catcgccacc
    17581 gccgggaatt gggctttcgc tattagaaat gcgatcgccg cccgtaaccg cagtcgccag
    17641 tggagtccgg aagtactccg caatctgaat caactctttt gggctggaga tggagatcag
    17701 ccacatcacc agaaaaaagg ccatcatcgc ggtcataaag tcggcataag caatcttcca
    17761 cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg
    17821 cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc
    17881 acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca
    17941 aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagtgt gactttgacg
    18001 cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa
    18061 ataaatccgt aagctaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc
    18121 gcccccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgac ccccattaca
    18181 gccgcgacaa taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg
    18241 acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag
    18301 gtgttcatgt gaccgctgat aatcaggcgc agataatcga cgataaaatc aagcatgacg
    18361 ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata
    18421 tcacgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga
    18481 gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc
    18541 gccttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct
    18601 gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc
    18661 atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg
    18721 acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga
    18781 ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gccacatcgt
    18841 atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga
    18901 ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca
    18961 aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg
    19021 gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc
    19081 agcagcggtc cttcttctgc ttgcgggcac tgttcaaggt ataaacggta ggctttgatc
    19141 accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc attacagaac
    19201 atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa tggcagcatg
    19261 cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt
    19321 aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc
    19381 agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttct
    19441 tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag agcatgatgc
    19501 cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaattga gtaatcgtct
    19561 ggtggctgtc aaaacggaag tgacaaacca gctgattggt ttctgccagc ttaaccattt
    19621 gcggaagagt cagtgccgct aatgttgtcg ccatttcttc atttatgccg agacgaaaca
    19681 tagcggacgc tttgtcctga acaatcagac gctgtgcaag tagtaaatat gacaagttga
    19741 tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag aataaccaac
    19801 tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat
    19861 aattagcatt agaatagttg cgataagctg caataagcag agccaccttt ttggtttaat
    19921 aagtccttac aaatagaagg gggtctttac acttatctaa gatttttcct aaatcgacgc
    19981 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caacaatgtg
    20041 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
    20101 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
    20161 aggaataatc gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcat
//
LOCUS       DAPGRN010000087        20051 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPGRN010000087 DAPGRN010000000
VERSION     DAPGRN010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.087"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..568
                     /locus_tag="RHW33_003304"
     CDS             50..568
                     /locus_tag="RHW33_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV1646780.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            584..1123
                     /locus_tag="RHW33_003305"
     CDS             584..1123
                     /locus_tag="RHW33_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV1646781.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="RHW33_003306"
     CDS             complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="RHW33_003306"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV1646782.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="RHW33_003307"
     CDS             complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="RHW33_003307"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV1646783.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4490..5860
                     /gene="xseA"
                     /locus_tag="RHW33_003308"
     CDS             4490..5860
                     /gene="xseA"
                     /locus_tag="RHW33_003308"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV1646784.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGKVLKKVKQVKAGEMLTTRLEDGWVESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5857..6072)
                     /locus_tag="RHW33_003309"
     CDS             complement(5857..6072)
                     /locus_tag="RHW33_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV1646785.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6142..7614)
                     /gene="der"
                     /locus_tag="RHW33_003310"
     CDS             complement(6142..7614)
                     /gene="der"
                     /locus_tag="RHW33_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV1646786.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="RHW33_003311"
     CDS             complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="RHW33_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV1646787.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="RHW33_003312"
     CDS             complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="RHW33_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV1646788.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="RHW33_003313"
     CDS             complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="RHW33_003313"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV1646789.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="RHW33_003314"
                     /gene_synonym="gcpE"
     CDS             complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="RHW33_003314"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV1646790.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="RHW33_003315"
     CDS             complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="RHW33_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV1646791.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13387..14541)
                     /gene="trmG/rlmN"
                     /locus_tag="RHW33_003316"
     CDS             complement(13387..14541)
                     /gene="trmG/rlmN"
                     /locus_tag="RHW33_003316"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV1646792.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="RHW33_003317"
     CDS             complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="RHW33_003317"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV1646793.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15263..16117)
                     /locus_tag="RHW33_003318"
     CDS             complement(15263..16117)
                     /locus_tag="RHW33_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV1646794.1"
                     /translation="MLSHNITIGAECARQRFRFATCHACVEACPAQALTLTEEGIAVD
                     AEQCIECAVCQFICPQEAIRGVNSPLRYFRNHTLVAPFTTIAPTLEELLLWHKEQGIT
                     AIAIDLSQAKAWERALLLLNERLRQYGEEGWSVVSPHTSEVNVSRRRLFHVQRADVQM
                     TQIQPGLRRLHYLWPQISDARPMMDPQKCQLCGACWRACEQQVFSLNEGHLQINDALC
                     NGCQNCIAVCFHQAMTVELTILPAKIVNLHANRKVCKTCQKSFLTFQQNAQNCLYCQR
                     HRYGMRTP"
     gene            complement(16117..16938)
                     /locus_tag="RHW33_003319"
     CDS             complement(16117..16938)
                     /locus_tag="RHW33_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     family protein"
                     /protein_id="HDV1646795.1"
                     /translation="MHELPLMAFTLLLQASVGMTLWLAFLHRHILITSSARPVMRPPL
                     LLAFLAGAVGLLISTLHLGYPLNAMHALSHFSSSWLSREIIFGALYLALLGLSTLLVM
                     LRKGGWQLLLILAAVVGIVDVFCMAQVYIHTSIITWQHYNTLIMFLGTVGILGSALVA
                     VFRISGILPQIDALRNGCVLVIALLVLLRLLVQPLWIGDLTANAMQIATLPHAPLAML
                     GQLKPILTLSWGISVIGMMFFAVGGCKKNIPAALFGSVMLVGSEVMLRFVFFSIG"
     gene            complement(16931..17560)
                     /gene="dmsB"
                     /locus_tag="RHW33_003320"
     CDS             complement(16931..17560)
                     /gene="dmsB"
                     /locus_tag="RHW33_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV1646796.1"
                     /translation="MSQFKEFASVSDKQLGFFIDSSRCSGCKACQVACKDKNNLEVGR
                     RFRRVYEVNGGGFIATGEGGVSHNVFAYTLSVSCNHCADPICTKNCPTMAMHKRPGDG
                     IVRVNTDKCVGCGYCAWSCPYGAPQMNEQTGQMSKCDFCIDLQAKGEQPICVATCPLG
                     AIKFGPIDELREKYGDVSDVKGLPDSSITQPNLVIKPHQGAEKEASHHA"
     gene            complement(17557..19938)
                     /locus_tag="RHW33_003321"
     CDS             complement(17557..19938)
                     /locus_tag="RHW33_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDV1646797.1"
                     /translation="MDTPDEKERITFPQVSRRSFLQATSALITLPFISSTAKAQSPDA
                     SPEVTAPVADKVVPTCSTFDCGGKCDIRAHMRDGVVTQITTLPDNELDPQMPIMRACV
                     RGRGYRKFVYHPDRLKYPMKRVGKRGEGKFERISWDEATTLIADNLKRITQQYGPASR
                     YVHVGTAVSGGTFSGDAMARRLLNLTGGYLEYYHSVSLGNTAAATPYTYGVAASGNSM
                     DTLLDTKLVILWGHNPTETIFGHTNYFFQKMKQNGTRFIVVDPRYSDTVSSLAEQWIP
                     LLPTTDNALMDAMMYVIISENLHNKTFIDTYTLGFDENSMPEGVPANESLVAYLFGAK
                     DGIHKTPEWAEKITHVPAQTIRQLARDYANTKPAALIQGWGPQRHICGERTARGSTLL
                     ASITGNVGIKGGWAAGYGGSSNRKFCVGPDMPENPVQAKISIMNWMQAADDASKVTPQ
                     DGLKGVDKLDSNIRLLFSLAGNYLANQNPDVHQAAKLLEDESKIEFIVLSDLFMTPSA
                     KYADMLLPETSFMERWNIGETWGTASYLILSEKLIEPDFERRTDYDWLRDVAKKLGVE
                     AEFSQGRDEKQWIEHIWEQTRLAMPDENLPDFATLQKTRRHLFKSAPHIAFEANIRDP
                     QNNPFPTPSGKIEIFSKRLFDMQDPEIPALSHYVPAFEGPEDKLTAKYPLQLITWKGK
                     NRANSTQYANPWLQEVQTQKLWLNPQDAKQRGISEGDSVKIYNDRGVSIIPVEITPRI
                     IPGVVAMQAGAWWQPDAQGIDRGGCANVLSSTRITALAKGNSHQTMLVEVEKA"
BASE COUNT         4981 a         5415 c         4865 g         4790 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actcttgcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcgggtctg gcagcaattc cggaacgacg gcaggtgcag ttggcggcgg
      361 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacgtat aaggaaggca ccaaagtgta tacctctact caggtgggta aagagtgcca
      481 gtttacgaca ggtttagccg ttgttattac cacgacgtat aacgaaacgc gtattcagcc
      541 aaataccaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      601 ttgcgccatc ttggcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      661 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      721 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      781 ggctgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
      841 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      901 cagcttagag attcagaaac aaaaactggc gctggcaaaa gaagaagccc gcgttaagcg
      961 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
     1021 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1081 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1141 aagcctataa ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1201 tagtgcaggg tcaaatcatt cccactcaat ggtagctggc ggcttgccgc tgatgtcata
     1261 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
     1321 atcgtacggc agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
     1381 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtactggcag
     1441 gaagacggtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat
     1501 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagaac
     1561 acgaacgcca aggcctggtc ccgggaacgg gtgacggtac agcatgtcgt acggcaggcc
     1621 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1681 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1741 tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1801 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgacc
     1861 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1921 ttctgccggt acgtgaacaa tgttaagacc aaagtgatcg ccaaacatat ccagaacctg
     1981 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc
     2041 gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag
     2101 gatgacttta tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt
     2161 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg
     2221 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     2281 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     2341 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     2401 tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg
     2461 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2521 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2581 cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa taatgccgct
     2641 tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt cgcagtaaac
     2701 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2761 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2821 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2881 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2941 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaga
     3001 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     3061 taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg
     3121 aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat
     3181 ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc
     3241 atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     3301 aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg
     3361 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     3421 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccag agcgcgtgca
     3481 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3541 tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaaca
     3601 tcaacgcctg cggcaaccag cgcatcaaca cgctcttcgt tacccgcacc tgcgccaact
     3661 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgctccgct
     3721 ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3781 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3841 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3901 gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc ttcggtaacg
     3961 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     4021 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     4081 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     4141 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     4201 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     4261 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     4321 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     4381 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cggagggcgg
     4441 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4501 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4561 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4621 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4681 caacagcaac cgccgggtga ccttccgccc acagcatggg caacaagttt tagttcgcgc
     4741 caatattacg ctctacgagc cgcgcggcga ctaccagata atcgttgaga gtatgcagcc
     4801 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4861 aggattgttc gatctgcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4921 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4981 tccttctctg ccggtgatca tctaccccac cgccgttcag ggcgatgacg cgccggggca
     5041 aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt tgatcgttgg
     5101 gcgcggtggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     5161 gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     5221 tgccgatttt gttgccgatc tgcgtgcacc aacaccgtcg gctgccgccg aagtggtgag
     5281 ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc tggagatggc
     5341 gatggattat tatctcgcca accgcacacg tcgctttacg caaattcatc accgattaca
     5401 gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa
     5461 acggatgagc tttgcgctgg aaaatcaact taagcgtgcc gggcaacagc agcagcgatt
     5521 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc aaacgcgcat
     5581 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgca cagcttagcg ccacgcgtga
     5641 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5701 tggatacagc gttactactg ctactgacgg caaggtactg aaaaaagtga agcaggttaa
     5761 agcgggtgaa atgctaacca cacgtctgga agacggctgg gtagaaagtg aagtaaaaaa
     5821 catccagccg gtaaaaaaat cgcgtaaaaa ggttcattaa gccagcacga attcaacgcg
     5881 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac cgcaggcttt
     5941 taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa taaattcccc
     6001 gcaggatggg caacgcgcat ggccgttatc ctgatcaagc acatgctgac attgtggaca
     6061 atgcagttcc atatgaattc ctccggtagc cattccctct acattcatag agggaatggc
     6121 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
     6181 tttacgcatc tgggttggcg tcagggtgtt acgcttattc gcatacgggt tttccccttc
     6241 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
     6301 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
     6361 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cacgtaccag
     6421 cggcggctgg tgatcttcaa ctgccatggt cataatacgt gtcagcatag acgttcccac
     6481 acgacgggtg gaactgtcat aagcttcacg cacagattca aacaggttac caacaccgct
     6541 accgtgcaat gcagagataa aatgtacgcg agcaaaatcg ataaagccca gacggaagtc
     6601 cagcgtttct ttcacctgct ctttcacttc ctgactcagg ccatcccact tattcaccac
     6661 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
     6721 aataccttcg cgcgcatcaa tcactaacat caccacgttg gcgtcttcaa tggcctgcaa
     6781 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
     6841 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
     6901 acgcgtcgta ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
     6961 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
     7021 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
     7081 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
     7141 cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg ggtagatttc
     7201 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
     7261 gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc
     7321 tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
     7381 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
     7441 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
     7501 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
     7561 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
     7621 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
     7681 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
     7741 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
     7801 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
     7861 gttaatccag tgcaaataac cttcactgtc accgaccacc aggttgccat tatacagcac
     7921 cggagaagtc agcaggcgat gcagcagatc gctttgtgtc cacagcgtaa cgccgccatc
     7981 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
     8041 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
     8101 aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa caacgacggg
     8161 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
     8221 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
     8281 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
     8341 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
     8401 ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc gcgaaagtgc
     8461 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
     8521 ctgcgccttt tcgctgccaa tgtagacatg tccaccagac acggtcacac cgccagaaag
     8581 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
     8641 tttgccatca tccgcattca gcgcttttac taaaccagcg cggtccgctg catagacaac
     8701 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
     8761 agtgctccac accgtggtcg gcgtaaactg gttttcaacg gtcggcaatg gggacatctt
     8821 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
     8881 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
     8941 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
     9001 atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg
     9061 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
     9121 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
     9181 catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
     9241 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
     9301 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
     9361 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
     9421 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
     9481 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
     9541 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
     9601 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
     9661 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
     9721 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
     9781 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
     9841 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
     9901 acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cggccgccca gttgttccac
     9961 aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac tgttagtcac
    10021 ccactcgaaa acggtacggt tgtagtaatc cagaccacgc accagacgct ggtttacggt
    10081 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    10141 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    10201 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    10261 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    10321 cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca
    10381 ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    10441 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    10501 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    10561 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    10621 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    10681 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    10741 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    10801 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    10861 gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg ggttcaatca tacacgggaa
    10921 gcgaggcgct tcccatcacg ttattatttt tcgacctgct gaacgtcaat tcgacgcgct
    10981 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    11041 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttttt gttgccgccg
    11101 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    11161 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    11221 ataacatcaa actcctgacg cgaacaggtc ggacaggcga tgaagttgat ccctcgcgaa
    11281 cggatacgaa gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    11341 gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc taaaccaatg
    11401 gcggatttta ctgccccgct gcgcgcacca ccggcttcgg tgatccccag atgcagcggc
    11461 tgatcgattt gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    11521 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atggcgcatg
    11581 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    11641 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    11701 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    11761 ccgtattccg ctactttcag cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    11821 acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    11881 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    11941 acatctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    12001 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    12061 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcatttaggg
    12121 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    12181 actggatctg tactgcggct ggcgcaccaa ttttcagttt gtacggtgcc tggccggtta
    12241 agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg gtagcatcag
    12301 tgacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    12361 gcgtggtcac accagcctga tcggttggca acggcgcagc accatctggc gttgttgttg
    12421 ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg cgaaggcgag acaaccgcat
    12481 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    12541 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc tgggtctgta gttgtcgacg
    12601 tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc gaagattgat
    12661 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    12721 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccgtcgcgtt
    12781 ttttgcggcg tttaccgagg gaaaaactct gcatcggcgc aacttttgca gcccgaagtg
    12841 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    12901 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    12961 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    13021 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    13081 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    13141 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    13201 ataagaccgt ctgtgtgctc gtgacaaagt ccacattcac taaggctaac ttactgttgc
    13261 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    13321 caacttaata cttcattcat aaaacattac gccacggtac ataaagtaac cgtggcgtaa
    13381 tgactatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    13441 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    13501 acgcgtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    13561 gatacggctg ttcgagctgc gtccatacgg cgcacccggg aacgggttcc acgggatcag
    13621 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgagcgt gttcagtgcc
    13681 gtcgttaacg tggtcaagca tcacgtattc aatagtgact cggccttgat tggcgttgga
    13741 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    13801 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagcgcaac
    13861 gtcgatcata tcgcccagtt tatccagcgc tggaactaca ccggaagtgg aaagcgtcac
    13921 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    13981 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattgggcg
    14041 ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccaaacct ggccgataat
    14101 ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    14161 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    14221 caccgtttcg acgcgctgat cgcctacggc gatcgcccat ttaatggtgc cgtcagatga
    14281 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    14341 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    14401 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    14461 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    14521 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    14581 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    14641 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    14701 cgcgggcaca cttcgccttc gccgaagaaa taagcgattt cgcgagcggc agattcgacg
    14761 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    14821 ccggccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    14881 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    14941 tcaaagaacg gttttccatc gtgttcagca taaaagccac gagcctgttc aacggtcagg
    15001 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    15061 ccaatgacgt tttttgctac cgcgttcggt ttgataatgg aaaaagtacg ttcaatagcc
    15121 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    15181 tataatgagc aacagagccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    15241 agttttagca acaactcgac ttttatggtg ttctcatccc ataacgatga cgttggcaat
    15301 aaagacaatt ttgcgcattt tgctgaaaag taagaaagga tttttgacag gttttacaga
    15361 ctttgcgatt agcatgaaga ttcacaattt ttgccggtag aatcgtcaat tcaacggtca
    15421 ttgcctgatg aaaacaaaca gcaatacaat tttggcatcc gttacacaag gcatcattta
    15481 tttgtaaatg gccttcgtta agcgaaaaga cctgttgctc gcaggcccgc cagcatgcac
    15541 cgcaaagctg acacttttga ggatccatca tcgggcgagc atcactaatc tggggccata
    15601 aataatgtaa ccgacgtaat cctggttgta tttgtgtcat ctgaacatcc gcacgctgca
    15661 catggaacaa acgacgccgg gaaacattca cctcactggt atgtggagat accacagacc
    15721 agccttcctc gccatattgc cgtaagcgtt cgtttaacag caataatgcg cgctcccatg
    15781 ccttcgcctg actcagatca atcgcgatcg ctgtgatccc ctgctcttta tgccatagca
    15841 aaagttcttc cagcgtcggc gcaatagtgg tgaaaggtgc gaccagggtg tgattgcgaa
    15901 aataacgtaa aggggaatta acgccacgaa tagcctcttg cggacaaata aactggcaaa
    15961 ccgcacactc aatacattgt tccgcgtcga ccgctatgcc ctcttctgtc agcgttagcg
    16021 cctgtgccgg gcaagcctcg acacaggcat gacatgttgc aaaacgaaag cgttggcgcg
    16081 cgcattccgc gccaatcgtt atgttatgac ttaacattag ccgatactaa aaaagacaaa
    16141 acgcagcata acttcactcc ccactagcat tacgctaccg aataacgccg ccgggatatt
    16201 ctttttacaa ccaccgacag caaagaacat catcccaatg acggaaattc cccagcttag
    16261 tgtcagtatg ggttttaact gtcccagcat cgctaatggc gcatgtggca atgtggctat
    16321 ctgcatcgca tttgccgtta aatcccctat ccacaacggc tgaaccaaca aacgcagcag
    16381 aaccaggaga gcgatcacca acacgcatcc attacgcagg gcatcaatct gcggcaaaat
    16441 tccgctaata cgaaacactg ctaccagcgc tgaaccgaga attcccactg tacctaagaa
    16501 cataatcagc gtgttgtaat gctgccaggt gataatcgac gtatggatgt aaacttgcgc
    16561 catgcagaaa acatcaacaa tcccgaccac tgccgctagg ataagcagga gttgccagcc
    16621 gcctttgcgt aacatgacca gtaatgtgga taaacccaat agcgccagat acagcgcacc
    16681 aaagataatt tcccgactca gccatgaact ggaaaagtgg cttagcgcat gcatcgcatt
    16741 aagtggataa cccagatgta aggtagaaat cagtaaccct acggcacctg ccaaaaaagc
    16801 caacagtaat ggcggccgca taaccgggcg ggcgctggag gtgattaaaa tatgacgatg
    16861 aagaaacgcc agccacaacg tcataccgac cgatgcctgt aacaacagcg taaaagccat
    16921 taacggtaat tcatgcatga tgtgacgcct ccttttccgc gccctgatgc ggtttaatca
    16981 ccagatttgg ctgcgtaatc gacgaatcag gtaaaccttt cacatcactg acatcgccat
    17041 acttttcacg caactcatca atagggccga acttaatcgc ccccaacggg cacgttgcca
    17101 cgcaaatggg ttgttctcct ttagcttgca gatcaataca gaaatcgcat ttcgacatct
    17161 gcccggtctg ctcattcatt tgtggtgcgc cgtaaggaca ggaccaggcg cagtaaccgc
    17221 aaccgacgca cttatcagta ttcacgcgca caataccgtc tccgggacgc ttatgcatcg
    17281 ccatcgtcgg gcaatttttg gtacagatcg gatccgcgca atggttacag gaaacagaaa
    17341 gggtataagc aaagacatta tgactcacac cgccctctcc agtggcgatg aagccaccgc
    17401 cgttcacttc atacacgcgg cgaaaacgtc gcccaacctc aaggttgttt ttgtctttac
    17461 aggccacctg acacgcttta cagcccgaac aacgcgaaga gtcaataaag aaccctaatt
    17521 gtttgtcgct gacagatgca aattctttaa attggctcat gctttctcaa cctccaccag
    17581 cattgtttga tgcgaattcc ctttcgccag cgccgtaatg cgggtcgaac tcagtacatt
    17641 ggcacagcct ccgcgatcga taccttgtgc atccggttgc caccatgccc ccgcctgcat
    17701 agcaactacg ccagggatga tacggggggt tatttccacc gggataatgc tcacaccacg
    17761 atcgttataa atttttacgc tatctccttc gctaatcccg cgctgttttg catcttgcgg
    17821 attcagccag agtttttgcg tctgaacttc ttgcagccac gggttagcat actgggtgga
    17881 attagcacgg tttttccctt tccaggtgat gagctgcagc gggtatttcg ccgtcagttt
    17941 atcttctggc ccttcaaagg cagggacata gtgcgaaagc gcgggaattt ctggatcttg
    18001 catatcaaaa agacgcttag agaagatctc aattttgccg gaaggcgtcg ggaaaggatt
    18061 attctgcgga tcgcgaatat ttgcttcgaa agcgatgtgc ggcgcgcttt tgaaaagatg
    18121 tcgacgtgtt ttttgcaggg tcgcgaagtc cggcaaattt tcatccggca tcgccagacg
    18181 agtttgctcc cagatatgct cgatccattg cttctcatca cgtccttgac tgaactcggc
    18241 ttcgacaccc agttttttcg ccacgtcacg caaccagtca taatcggtgc gacgttcaaa
    18301 atccggctca atcagttttt ctgacagaat aagatagctc gcagttcccc atgtttcgcc
    18361 gatattccag cgttccataa agctggtttc cggcagcaac atatcggcat atttcgcgct
    18421 cggagtcatg aacagatcgc tgagtacgat gaattctatt ttagattcgt cttcaaggag
    18481 cttcgcggcc tggtggacgt ccgggttctg gttcgccaga taattacctg ccagtgagaa
    18541 cagcaggcgg atattactgt cgagtttatc gacaccttta agcccatctt gcggcgtaac
    18601 tttagaagca tcatccgccg cctgcatcca gttcataatc gagattttcg cttgtaccgg
    18661 attttctggc atatctggtc caacgcagaa tttacgatta gaactaccgc cgtaacctgc
    18721 cgcccagcca cctttgatgc cgacgttacc ggtaatcgag gccagtaaag ttgaacctcg
    18781 ggcggtgcgt tcgccgcaaa tatgtcgctg cggtccccat ccctgaatca gagctgcagg
    18841 tttggtgttg gcgtaatcgc gtgccagttg acgaatggtt tgcgctggaa cgtgggtaat
    18901 tttttctgcc cattccggcg ttttgtgtat cccatctttt gcgccaaaaa ggtaggcaac
    18961 caacgattcg ttggctggta cgccttctgg catagagttt tcatcaaaac cgagagtata
    19021 tgtgtcgata aatgtcttat tgtggagatt ttcgctgata atgacgtaca tcatcgcgtc
    19081 catcaacgca ttgtccgtgg tcggcagcag cgggatccac tgttcggcaa gcgaagatac
    19141 agtgtcggaa taacgcggat ctaccacgat aaaacgcgta ccgttttgct tcattttttg
    19201 aaaaaagtaa ttggtgtgac cgaagatggt ttccgttggg ttatgccccc agaggatcac
    19261 cagtttggta tccagaagag tatccatcga gttaccgctg gccgccacgc catacgtgta
    19321 aggcgttgcc gccgcagtat tacccagact aactgagtga taatattcaa gataaccacc
    19381 ggttaaattt aacagccgac gcgccatcgc atcaccagaa aatgttccac cagaaacagc
    19441 cgtcccgaca tggacataac gtgaagccgg gccatattgt tgcgtgatcc ttttcaggtt
    19501 atcggcaatt aaggtcgtcg cctcatccca tgagatacgc tcaaatttac cctcgcctcg
    19561 tttacctacc cgcttcatcg gatatttcaa acggtctgga tgataaacaa atttacgata
    19621 gccgcgacca cgaacacagg cgcgcataat cggcatttgt ggatccaatt cgttatcggg
    19681 aagcgtggta atttgcgtca ccacgccgtc gcgcatatgc gcccgaatat cgcacttacc
    19741 accgcagtca aatgtgctac aggtgggcac gactttatca gctactggag ctgtaacttc
    19801 tggcgacgca tctggggatt gcgcctttgc ggtagaagaa ataaaaggca aagtgataag
    19861 cgcggaagtc gcttgtaaaa aactacgtcg tgatacttgc ggaaatgtaa tgcgttcctt
    19921 ttcgtctggc gtatccatct tgcgttcccg aaaatgaaaa aataaatatt cacgtattta
    19981 ttcgagaaca atgctgataa caatcaaata attactcatt ttaaaaaaac gtgagattat
    20041 taaattatta a
//
LOCUS       DAPGRN010000088        20003 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.088, whole genome shotgun sequence.
ACCESSION   DAPGRN010000088 DAPGRN010000000
VERSION     DAPGRN010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.088"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(619..703)
                     /locus_tag="RHW33_003322"
     tRNA            complement(619..703)
                     /locus_tag="RHW33_003322"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(667..669),aa:Leu,seq:caa)
     gene            899..1918
                     /gene="ahr"
                     /locus_tag="RHW33_003323"
     CDS             899..1918
                     /gene="ahr"
                     /locus_tag="RHW33_003323"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV1646798.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            complement(1922..2485)
                     /gene="idnK"
                     /locus_tag="RHW33_003324"
     CDS             complement(1922..2485)
                     /gene="idnK"
                     /locus_tag="RHW33_003324"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV1646799.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            2702..3733
                     /gene="idnD"
                     /locus_tag="RHW33_003325"
     CDS             2702..3733
                     /gene="idnD"
                     /locus_tag="RHW33_003325"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HDV1646800.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3757..4521
                     /gene="idnO"
                     /locus_tag="RHW33_003326"
     CDS             3757..4521
                     /gene="idnO"
                     /locus_tag="RHW33_003326"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDV1646801.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4586..5905
                     /gene="idnT"
                     /locus_tag="RHW33_003327"
     CDS             4586..5905
                     /gene="idnT"
                     /locus_tag="RHW33_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HDV1646802.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            5972..6970
                     /gene="idnR"
                     /locus_tag="RHW33_003328"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             5972..6970
                     /gene="idnR"
                     /locus_tag="RHW33_003328"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HDV1646803.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRTAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7048..8550
                     /locus_tag="RHW33_003329"
     CDS             7048..8550
                     /locus_tag="RHW33_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV1646804.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASNEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8711..9793)
                     /gene="lptG"
                     /locus_tag="RHW33_003330"
     CDS             complement(8711..9793)
                     /gene="lptG"
                     /locus_tag="RHW33_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV1646805.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9793..10893)
                     /gene="lptF"
                     /locus_tag="RHW33_003331"
     CDS             complement(9793..10893)
                     /gene="lptF"
                     /locus_tag="RHW33_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV1646806.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWIV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11160..12671
                     /gene="pepA"
                     /locus_tag="RHW33_003332"
     CDS             11160..12671
                     /gene="pepA"
                     /locus_tag="RHW33_003332"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV1646807.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13025..13468
                     /gene="holC"
                     /locus_tag="RHW33_003333"
     CDS             13025..13468
                     /gene="holC"
                     /locus_tag="RHW33_003333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV1646808.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13468..16323
                     /gene="valS"
                     /locus_tag="RHW33_003334"
     CDS             13468..16323
                     /gene="valS"
                     /locus_tag="RHW33_003334"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV1646809.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEELIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16379..17575)
                     /locus_tag="RHW33_003335"
     CDS             complement(16379..17575)
                     /locus_tag="RHW33_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV1646810.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCRRYLYANMEVNGQRFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
     gene            17768..18271
                     /locus_tag="RHW33_003336"
     CDS             17768..18271
                     /locus_tag="RHW33_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1646811.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(18317..18733)
                     /gene="rraB"
                     /locus_tag="RHW33_003337"
     CDS             complement(18317..18733)
                     /gene="rraB"
                     /locus_tag="RHW33_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV1646812.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            18895..19908
                     /gene="argF"
                     /locus_tag="RHW33_003338"
     CDS             18895..19908
                     /gene="argF"
                     /locus_tag="RHW33_003338"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV1646813.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         5150 a         4931 c         5112 g         4810 t
ORIGIN      
        1 ttacaccttg catttttata accaatgaaa tctacaacaa gcaactgatg gataatccga
       61 tattggtcgc atttaatggc atggtagcaa agttttcatc acatacctcc tttgcaccag
      121 aataaacgcc agagatctga aattgcgagt acactacaga aattgtactg ccggtcccat
      181 cagcataaaa ccatagttac acaggtaatc atcgttattc gccaagataa taaatattat
      241 tcggtagaat agcgttctca ggtggtcagt aaaattgtct tcaacaagat gtctgcgcat
      301 ctcgtctgct agccctacat aactgctctc gaccgaaact atttattgaa tgttcaccaa
      361 ggtgggcgtg gagacaaaat gacgtctttt gatattagct acctgagcca catggtcaaa
      421 acgttcaagt agcatagcga aaccaccctg cccacgtaat gtggacgatg cagcttccag
      481 ccagagcaaa cttgggacca tcgactacaa aatgaagaat actgaactgc ttttctggca
      541 ggcctatata ctgcacggat atgggtgatt tcagacacaa aaaaagccgc tcttgagcga
      601 ctcgatttgc atacggtgtg gtgccgaagg ccggactcga accggcacgt atttctacgg
      661 ttgattttga atcaactgcg tctaccgatt tcgccacttc ggcactgaag aggtatgcgg
      721 aaaacgttgt gaattatacc tgtcgcacgc caccatgcaa gcgccagctt gctcgcacct
      781 cgctaagtgt tgaaattttc agcattacac tcagcgatgt gtaacctttg tcacactcca
      841 ggcaccccgc cctgccatgc tctacacttc ccaaacaaca ccagagaagg accaaaaaat
      901 gtcgatgata aaaagctatg ccgcaaaaga agcgggcggc gaactggaag tttatgagta
      961 cgatcccggt gagctgaagc cacaagatgt tgaagtgcag gtggattact gcgggatctg
     1021 ccattccgat ctgtcgatga tcgataacga atggggattt tcgcaatatc cgctggttgc
     1081 cgggcatgag gtgattggtc gcgtggtggc gctcgggagc gccgcgcagg ataaaggttt
     1141 gcaggtcggt cagcgtgtcg ggattggctg gacggcgcgt agctgcgggc attgcgatgc
     1201 ctgtattagc ggtaatcaga tcaactgtga gcaaggtgcg gtgccgacga ttatgaatcg
     1261 cggcggcttt gccgagaagt tgcgtgcgga ctggcaatgg gtgatcccac tgccagaaaa
     1321 tattgatatc gagtccgccg ggccgctgtt gtgcggtggt atcacggtct tcaaaccact
     1381 gttaatgcac catatcactg ccaccagccg cgttggggta attggtattg gcgggctggg
     1441 gcatatcgct ataaaacttc tgcacgcaat gggatgcgag gtgacagcct ttagttctaa
     1501 tccggcgaaa gagcaggaag tactggcgat gggtgccgat aaagtggtga atagccgcga
     1561 tccgcaggca ctgaaagcac tggcggggca gtttgatctc attatcaaca ccgtcaacgt
     1621 cagcctcgac tggcagcctt attttgaagc gctgacgtac ggcggtaatt tccatacggt
     1681 cggtgcagtt ctcacgccgc tgtctgttcc ggcctttacg ttaattgcgg gcgaccgcag
     1741 cgtctctggt tctgctaccg gcacgcctta tgagttgcgt aagctgatgc gctttgccgc
     1801 ccgcagcaag gttgcgccga ctaccgaatt gttcccgatg tcgaaaatta acgacgccat
     1861 tcagcatgta cgcgatggta aggcgcgtta ccgcgtggtg ttgaaagccg atttttgaaa
     1921 atcattcgca gcgctgatct gaggcgctgc cctctttcgc acatattctg ttttgtcgta
     1981 tcgccagcac agcctgccga cattgttcgg tgacattggc aatatcatgg ttgatatcaa
     2041 tgcgcacaat atcctgttca tctgcttgtg gacgctccag tgcctcaaac tgacttttta
     2101 gtaacgctac cggcataaaa tgcccagccc gacgctgcat tcgcgcgaga atagtttcat
     2161 agtcgccatc taaccagagg aaatgaacat gggggctacc cttgcgtaaa atatcacgat
     2221 actgtttttt taatgacgaa cagacaataa atcctgtttc attcttttta taaagactgt
     2281 atgaagcatc atttaagcgt tccagccagg gaagtcgatc ttcatcagat aatggaatac
     2341 cctgcgacat tttatctata tttttggctg gatgaagatc gtcaccatca ataaatttag
     2401 cagataataa cgcggcaacc ttgctaccaa ttaatgtttt accactccct gaaacgccca
     2461 tcaaaataaa gctttcaccc gccatttaaa atctcctgaa tcatcttacc tgctgcgata
     2521 ctacgcctgg cggcataata atttcacgtt tttttatttc tgctgataag aattacaagg
     2581 cacatcacgt tatgcgtaac atagtaatgt aacaattttc tgacgtgatc ttcatcacaa
     2641 ataatgacaa ttaaaaccgc ttaaatgctt ccgaggtgtc tacctgacca gtgaggaatt
     2701 tatgcaagtg aaaacacagt cctgcgttgt tgcgggcaag aaaactgttg ccgttaccga
     2761 gcagacgata gattggaata ataatggaac attagtacaa ataacccgag gtggaatttg
     2821 cggttccgat ttacattatt atcaggaagg aaaagtaggt aatttcatga taaaggcacc
     2881 gatggtgtta ggtcatgaag ttatcggtaa agttattcat agcgactcat cagaattaca
     2941 tgaagggcaa acggtagcca ttaatccgtc taaaccgtgc ggtcactgca aatactgcat
     3001 tgaacataac gagaatcagt gtacagatat gcgttttttt ggcagtgcca tgtatttccc
     3061 tcatgttgat ggtggtttta cccgttataa aatggtcgaa acgtcgcaat gtgtccctta
     3121 tccggccaaa gctgacgaaa aggttatggc ttttgccgaa cctttagccg tcgcgattca
     3181 tgccgcacat caggccggcg agttacaggg caagcgagta tttatttccg gtgttggacc
     3241 cattggctgc ctgattgtca gtgcagtgaa aacactgggg gccgcggaaa ttgtctgtgc
     3301 tgatgtgagt ccccgttccc tttcgctggg caaagagatg ggggcggatg tgctcgtaaa
     3361 cccacaaaac gacgacatgg atcactggaa agcggaaaaa ggctatttcg atgtcagctt
     3421 tgaagtgtcc ggtcatcctt catcagtgaa tacctgtctg gaggtcactc gtgcacgcgg
     3481 cgtaatggtg caggtaggta tgggtggcgc gatggcagaa ttcccaatga tgacgttgat
     3541 tggtaaggag atttcactca gaggctcttt ccgttttacc agcgaattta ataccgcagt
     3601 gtcatggctg gcgaatggcg ttatcaatcc actgccttta ctgagtgctg aatatccctt
     3661 cactgacttg gaagaggcgc tacgtttcgc cggtgataaa acccaggcag caaaagtcca
     3721 gcttgttttc taagagataa ataaaggaat aatacaatga acgatctatt ttcactggca
     3781 ggaaaaaata tcttgattac cggttcagca cagggcattg gctttttact ggcaaccggc
     3841 ctgggtaaat atggcgcaca aataattatt aatgatatta ctgccgaacg cgcagaactt
     3901 gctgtagaaa aactccacca ggagggtatt caggccgttg ccgcaccttt taatgttact
     3961 cataaacatg aaattgatgc cgccgttgaa catatcgaaa aggacatcgg ccccattgat
     4021 gtgctggtga ataacgccgg tatccagcgc cgtcatcctt ttactgagtt ccctgaacaa
     4081 gagtggaatg atgtgatcgc agtaaaccag accgccgtgt tcctggtatc gcaagcggta
     4141 actcgtcaca tggttgaacg caaggcaggt aaagttatta atatttgctc gatgcaaagc
     4201 gaactgggac gtgacaccat caccccttat gccgcatcga aaggggcggt aaaaatgctc
     4261 acccgcggca tgtgcgtcga gctggcgcgc cacaatattc aggtcaacgg tattgcgccg
     4321 ggctatttca aaacagaaat gactaaagca ctggttgagg acgaagcctt caccgcctgg
     4381 ttgtgcaaac ggacccccgc cgcacgctgg ggagatccgc aggaactgat tggtgctgcg
     4441 gtgttccttt cttcaaaagc ctctgatttc gtaaacggcc acctgttgtt tgttgatggc
     4501 ggcatgttag tggctgttta acacctacta ccctacgtgc agcgcgaaag cgctgctttt
     4561 ctggcactaa taagagataa agactatgcc attaatcatt attgcggcag gcgtcgcgct
     4621 gcttcttatc ctgatgatcg gctttaaagt taacggcttt attgccctcg ttctggtagc
     4681 tgccgtcgtc ggatttgccg aagggatgga tgcacaggcc gtcctgcact ctatacaaaa
     4741 tggtatcggc agcacgctcg gcgggctggc aatgatcctc ggtttcgggg ccatgttagg
     4801 caagctgatt tctgatacgg gtgcggcaca acgtatcgcc actacgctga ttgctacttt
     4861 tggtaaaaaa cgcgtgcaat gggcgctagt gatcaccggt ctggttgtgg gcctcgccat
     4921 gttttttgaa gtgggttttg tcctgctgtt gccgttggta tttaccatcg tagcatcatc
     4981 aggattaccc ctgttgtatg ttggcgtacc aatggtagca gcgctctctg taacccactg
     5041 ttttctgccg ccacatccag ggcctactgc catcgcgact atctttgagg ctaatctcgg
     5101 aacgacttta ctgtatggat ttatcattac cattccgaca gttattgtcg caggaccgct
     5161 gttttctaaa ctgctaactc gctttgagaa agcaccaccg gaaggcttat ttaatcctca
     5221 tctgtttagc gaagaggaga tgccctcctt ctggaacagt attttcgctg ccgtgatccc
     5281 ggtcatcctg atggctatcg ccgccgtttg tgaaattacg ttaccgaaaa ctaacaccgt
     5341 gcgcctcttc tttgaatttg tcggtaaccc tgccgttgcg ctgtttattg ccattgttat
     5401 tgcgattttc acactgggcc gacgtaatgg acgcaccatc gagcaaatca tggatatcat
     5461 tggggattct ataggcgcta tcgcgatgat tgtgtttatt atcgctggcg gcggcgcgtt
     5521 taagcaggta ttagtagata gcggtgtcgg gcagtatatt tcacacttaa tgaccggaac
     5581 tacgctttcg ccgttattga tgtgctggac tgttgcggcg ctgttgcgta tcgctctggg
     5641 ctctgccacc gtcgcggcca ttaccaccgc gggtgtggtg ttgccgatta tcaacgttac
     5701 ccatgccgat cccgctttaa tggtactggc aaccggtgcg ggcagtgtga tcgcgtcaca
     5761 cgtaaacgac cctggcttct ggctatttaa agggtatttt aatctgacgg ttggtgaaac
     5821 gttgcgtacc tggacggtga tggaaaccct tatttctatt atgggtttgc tgggcgtgtt
     5881 agccattaac gccgtattgc actgaaagaa taaaatattg tgatgtagtt tcgggggaag
     5941 attgaagatt cccccgacgt tgggagaagt tatgcgcaat cacagaattt ctttacagga
     6001 tatcgctacg ctggctggcg taacaaaaat gaccgtgagt cgttatatcc gctcgccgaa
     6061 aaaggtggca aaggaaacag gcgagcgcat cgccaaaatt atggaagaga tcaattatat
     6121 ccctaatcgc gctcccggca tgttgttgaa tgcacaaagt tataccctcg gtatcttgat
     6181 tccgtcattt caaaatcaac tcttcgctga cattctcgcg ggaatagaat ccgtcacctc
     6241 tgagcataat tatcagaccc ttattgctaa ttacaattac gatcgcgatt ccgaagaaga
     6301 gtcggtgatt aacctgctct cttataacat tgacgggatt atactttcgg aaaaatatca
     6361 caccatcagg acggtgaaat ttctgcgttc agccaccatt ccggtcgtgg aattgatgga
     6421 tgtacaggga gaacggctgg atatggaggt cggttttgat aatcggcagg ccgcttttga
     6481 catggtgtgt accatgctgg agaagcgagt caggcacaaa attctgtatc tcggttccaa
     6541 agatgacacc cgcgatgaac agcgttatca ggggtattgc gatgcgatga tgctgcataa
     6601 tctttcccca ttacgcatga atccacgcgc catctcatcc attcatttag gaatgcagtt
     6661 aatgcgtgat gcccttagcg caaacccgga tttagacggt gtattttgca ccaatgacga
     6721 tattgcgatg ggtgcgcttc tgctgtgccg cgagcgaaac ctcgccgtgc cagaacaaat
     6781 ttccattgcg ggttttcatg ggctggagat tggcaggcaa atgatcccaa gcctcgccag
     6841 cgtaattact ccccgttttg atattggtcg gacggctgcc caaatgttgc tgagcaaaat
     6901 taaaaataac gatcacaacc acaacactgt tgacctcggg tatcagattt atcacggcaa
     6961 cacgctttag gttattttct cttcattaca cgtattctct ttgccggacg ttgcctatac
     7021 tccaggcagg aaactggagg aaatctcatg agcgaacccc tgttaattgc ccgcacgccg
     7081 gacacagaac tgtttttact gccgggaatg gctaaccgtc acgggctgat tactggcgca
     7141 acggggacgg gtaaaactgt cacgttgcaa aaactggcag agtctctatc ggaaatcggt
     7201 gttccggtgt ttatggccga tgtgaaaggc gatctgaccg gtatcgcgca ggcaggaacg
     7261 gcgtcggaaa aactgctcac aaggcttaaa aatatcggcg tcaatgactg gcaaccgcat
     7321 gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag gccatccggt gcgggcgacg
     7381 gtttcggatc tggggccgct gttgctggcg cggctgttga atctcaacga tgtgcaatct
     7441 ggcgtgctga atatcatctt ccgcattgct gacgatcagg ggctgttact gctcgacttt
     7501 aaagatttgc gggcgattac ccagtacatc ggcgataacg ccaaatcctt ccagaatcag
     7561 tacggtaata tcagtagcgc atcggttggt gccatccagc gcgggctgtt gtcgctggaa
     7621 cagcaaggcg cagcacactt ctttggtgag ccgatgctgg atatcaaaga ctggatgcgc
     7681 accgatgcca acggtaaagg cgttatcaat atcctcagcg ccgagaagct ttatcagatg
     7741 ccgaaactgt atgccgccag cctgttgtgg atgctctcgg agttgtatga acaattgccg
     7801 gaagcgggcg atctggagaa accaaaactg gtgtttttct tcgacgaagc acatctgctg
     7861 tttaacgatg caccgcaggt actgctggat aagattgagc aggtgataag gcttattcgc
     7921 tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt ctgatattcc agataatgtg
     7981 ctcggccagc tcggtaatcg cgttcaacac gctttgcgtg cttttacgcc caaagatcag
     8041 aaagcggtaa aagctgcggc gcaaaccatg cgggctaatc cggcgtttga taccgaaaag
     8101 gcgattcagg aactgggcac cggcgaggcg ttaatctcgt ttctcgatgc gaaaggaagc
     8161 ccttctgtgg tggagcgtgc gatggtgatc gcgccttgtt cgcggatggg gccggtgacg
     8221 gaagatgagc gtaatggctt gattaatcac tctccggtgt atggcaagta cgaggatgac
     8281 gtggaccggg aatccgccta tgagatgttg caaaaaggct ttcaggccag taacgagcag
     8341 caaaataatc cccccgcgaa agggaaagag gtagcggtag atgacggcat tcttggtgga
     8401 ttgaaggata ttttgttcgg cactaccgga ccacgcggcg ggaagaaaga tggtgttgtg
     8461 caaacaatgg cgaaaagcgc cgcccgccag gtgacgaatc agattgtacg cgggatgttg
     8521 gggagtttgc tgggggggag aaaaaggtaa gccaggtgcg atttgtcttt gcctgatgcg
     8581 acgctggcgc gtcttatcat gcctacacca ctgcaatata ttggattttc ataatttttc
     8641 aggaatgaac gaagcacact ttgctaataa taataaaggg agctttcgct ccctttatgc
     8701 gttcattaga ttacgatttt ctcattaaca gccacaggct gattaagaag aagctggcgc
     8761 ttggcaacag tgcgccgatg atcggcggga tgccgtaaac caacgtcagc ggaccgaaga
     8821 tctggtccag tacgtagaag acaaaaccga agctgatacc ggtgaccaca cgcacgccca
     8881 tcggtacgct acgcagtggg ccaaagatga acgacagagc catcaacatc atcaccgcca
     8941 cggatagcgg ctggaagatt ttgctccaca tgttgagctg ataacgtccg gcatcctgac
     9001 cgctcgactt cagatacttc acgtagttgt gcaaaccact gatggagagt gcatccgggt
     9061 ccagcgccac cacgcccagt ttgtccggcg tgaggttggt tttccaggtg ccgctcaccg
     9121 tctgcgaacc ggtgatctgt ttcggattgg tcagatcaga ttcatcaacc tgcgacagac
     9181 gccagacttt atgttccggg tcaaacttcg cggtagcggc atagcgtacg gattgcagac
     9241 gacgattctc gttaaaggca taaatactga tgccacctaa ctcttcgtca cctttaaccc
     9301 gctcaatgta gacgaagttg ttgccatctt tcgcccataa accttgctgg gtagagagca
     9361 acgagccgcc gtacatcgcc tgcgcacggt agttacgcgc catctgctcg ccctgcggcg
     9421 cgacccattc gccaatcgcc atcgtcagta agaccagcgg aatggcggtt ttcatcaccg
     9481 acagcgccac ctgcatacgg gtaaaaccag aagcctgcat caccaccagt tcgctgcgct
     9541 gcgccagcat cccaagacca agcaacgccc caagcagagc cgccatcggg aagaagatct
     9601 gcacatcttt cggcacgctc agcaaggtat acattcctgc gcctaacgcg tcgtaactcc
     9661 cctgcccggc ttttttcaac tgatcgacaa acttgataat gcccgacagc gacaccagca
     9721 tgaacagcgt catcatgatg gtggtgaaaa tagttttacc gatatagcgg tcaagtacgc
     9781 caaaaggttg catcacaccg ctcctttacg cgaaaaactg gcgcgcaggc ggcggaccgg
     9841 cacggtgtcc caaaggttga gaacaatcgc caaagccaga taaatcaggt taacgatcca
     9901 catccacagc gtcgggtcca gcttaccttt accgccgttc gatttcaggg aggtctggat
     9961 caggaagaaa agtagataca gcagcatggc tggcagcatc gacagtacgc gtccctgacg
    10021 tgggttaacc acgctcagcg gtacgaccat aagtgccatc ataaacacgg tgaataccaa
    10081 cgtgatacgc cagttcagtt ctgcgcgagc acgatcggtg tcagtgttcc acaatgtgcg
    10141 catgtccatc tggtcggtat cgttcgggtc gagcgccacc gcctggtgac caatgatcgc
    10201 ctgataatcc tggaaatccg taatgcggaa atcacgtaac agtgcagtgc cttcgaagcg
    10261 cgttccctgg ttgagagtga cgacctggga gccgtcgcgc agctgggtta aatgtccgga
    10321 atcggccacc accacagaag gacgtgcatt accttttggt cgaatttgcg cgaggaacac
    10381 atctttgaaa tcgctgccgt caacgctttc gatgaacagc accgaactgc cattagtcgc
    10441 ttgctggaat tgcccttgcg ccagcgccgc catgccaggg ttcgctttcg cttctgctaa
    10501 cacttcatcc tgatgacgcg atgaccacgg tcccgcccac atcacgttaa ccgccgcgac
    10561 gattgccgtg aataccgcaa ggatcattgc cgctttcacc agaaccgctt tgctcaggcc
    10621 gcaggcatgc attaccgtaa tttcactttc ggtatacagt ttgcccagcg tcatcagcag
    10681 cccgaggaac aggcttaatg gcaggataag ctgcgccatt tccggcacgc ccaacccgag
    10741 aagggagagc accagattcg ccggaatatc gccgtcaacc gctgcgccga ggatcctcac
    10801 taacttttga cagaagaaga tcaaaagcaa gatgaagagt atcgccagct ggcttttgag
    10861 cgtctcccgc accagatatc ttatgattat cactttaaat acgcccgtaa aaactcgtct
    10921 tttgcaggat tttagcttgt ttcatggctt aaacgtcatt tattctcttg agtcgtcgaa
    10981 atcgtcgcta agataattat actcaacgga ttcacctctc agattttgtt ctgacgtgcc
    11041 aatgccgtaa taacgttaag attaacacga agtcatcgca acagcggaca tgagttacga
    11101 aagcttgcaa ttctatctgt agccaccgcc gttgtcttta agattcagga gcgtagtgca
    11161 tggagtttag tgtaaaaagc ggtagcccgg agaaacagcg gagtgcctgc atcgtcgtgg
    11221 gcgtcttcga accacgtcgc ctttctccga ttgcagaaca gctcgataaa atcagcgatg
    11281 ggtacatcag cgccctgcta cgtcggggcg aactggaagg aaaaccgggg cagacattgt
    11341 tgctgcacca tgttccgaat gtactttccg agcgaattct ccttattggt tgcggcaaag
    11401 aacgtgagct ggatgagcgt cagtacaagc aggttattca gaaaaccatt aatacgctga
    11461 atgatactgg ctcaatggaa gcggtctgct ttctgactga actgcacgtt aaaggccgta
    11521 ataactactg gaaagtgcgt caggctgtcg agacggcaaa agagacgctc tacagtttcg
    11581 atcagctgaa aacgaacaag agcgaaccgc gtcgtccgct gcgtaaaatg gtgttcaacg
    11641 tgccgacccg ccgtgaactg accagcggtg agcgcgcgat ccagcacggt ctggcgattg
    11701 ccgccgggat taaagcagcg aaagatctcg gcaatatgcc gccgaatatc tgtaacgccg
    11761 cttacctcgc ttcacaagcg cgccagctgg ctgacagcta cagcaagaat gttatcaccc
    11821 gcgttatcgg cgaacagcag atgaaagagc tggggatgca ttcctatctg gcggtcggtc
    11881 agggttcgca aaacgaatcg ctgatgtcgg tgattgagta caaaggcaac gcgtcggaag
    11941 atgcacgccc aatcgtgctg gtgggtaaag gtttaacctt cgactccggc ggtatctcga
    12001 tcaagccttc agaaggcatg gatgagatga agtacgatat gtgcggtgcg gcagcggttt
    12061 acggcgtgat gcggatggtc gcggagctac aactgccgat taacgttatc ggcgtgttgg
    12121 caggctgcga aaacatgcct ggcgggcgtg cctatcgtcc gggcgatgtg ttaaccacca
    12181 tgtccggtca aaccgttgaa gtgctgaata ccgacgctga aggccgcctg gtactgtgcg
    12241 acgtgttaac ttacgttgag cgttttgagc cggaagcggt gattgacgtg gcgacgctga
    12301 ccggtgcctg cgtgatcgcg ctgggtcatc atattactgg tctgatggcg aaccataatc
    12361 cgctggccca tgaactgatt gccgcgtctg aacaatccgg tgaccgcgca tggcgcttac
    12421 cgctgggtga cgagtatcag gaacaactgg agtccaattt tgccgatatg gcgaacattg
    12481 gcggtcgtcc tggtggggcg attaccgcag gttgcttcct gtcacgcttt acccgtaagt
    12541 acaactgggc gcacctggat attgcaggaa ccgcctggcg ttctggtaaa gcaaaaggcg
    12601 caaccggtcg tccggtagcg ttgctggcac agttcctgtt gaaccgcgct gggtttaacg
    12661 gcgaagagta attgcgtcag gcaaggctgt tattgccgga tgcggcgtga acgccttatc
    12721 cgacctacac agcactgaac tcgtaggcct gataagacgc gcaagcgtcg catcaggcaa
    12781 tgctgttatt gtcggatgcg gcatgaacgc cttatccgac ctacacagca ccgaactcgt
    12841 aggcctgata agacgcggca gcgtcgcatc aggcaatgct gttattgtcg gatgcggcgt
    12901 gagcgcctta tccgacctac acacagcact gaactcgtag gcctgataag acacaacagc
    12961 gtcgcatcag gcgctgcggt gtatacctga tgcgtattta aatccaccac aagaagcccc
    13021 atttatgaaa aacgcgacgt tctaccttct ggacaatgac accaccgtcg atggcttaag
    13081 cgccgttgag caactggtgt gtgaaattgc cgcagaacgt tggcgcagcg gtaagcgcgt
    13141 gctcatcgcc tgtgaagatg aaaagcaggc ttaccggctg gatgaagccc tgtgggcgcg
    13201 tccggcagaa agctttgttc cgcataattt agcgggagaa ggaccgcgcg gcggtgcgcc
    13261 ggtggagatc gcctggccgc aaaaacgcag cagcagcccg cgcgatatat tgattagtct
    13321 gcgaacaagc tttgcagatt ttgccaccgc tttcacagaa gtggtagact tcgttcctta
    13381 tgaagattct ctgaaacaac tggcgcgcga acgctataaa gcctaccgcg tggctggttt
    13441 caacctgaat acggcaacct ggaaataatg gaaaagacat ataacccaca agatatcgaa
    13501 cagccgcttt acgagcactg ggaaaagcag ggctacttta agcctaatgg cgatgaaagc
    13561 caggaaagtt tctgcatcat gatcccgccg ccgaacgtca ccggcagttt gcatatgggt
    13621 cacgccttcc agcaaaccat catggatacc atgatccgct atcagcgcat gcagggcaaa
    13681 aacaccctgt ggcaggtcgg tactgaccac gccgggatcg ctacccagat ggtcgttgag
    13741 cgcaagattg ccgcagaaga aggtaaaacc cgtcacgact acggccgcga agctttcatc
    13801 gacaaaatct gggaatggaa agcggaatcc ggcggcacga ttacccgtca gatgcgccgt
    13861 ctcggcaact ccgtcgactg ggagcgtgaa cgcttcacga tggacgaagg cctgtccaat
    13921 gcggtgaaag aagttttcgt tcgtctgtat aaagaagagc tgatttaccg tggcaaacgc
    13981 ctggtaaact gggatccgaa actgcgcacc gctatctctg acctggaagt ggaaaaccgc
    14041 gaatcgaaag gttcgatgtg gcacatccgc tatccgctgg ctgacggcgc gaaaaccgca
    14101 gacggtaaag attatctggt ggttgcgact acccgtccgg aaaccctgtt gggcgatacc
    14161 ggcgtggccg ttaacccgga agatccgcgt tacaaagacc tgattggcaa atatgtcatt
    14221 ctaccgctgg ttaaccgtcg tattccgatc gttggcgacg aacacgccga catggaaaaa
    14281 ggcaccggct gcgtgaagat caccccggcg cacgacttta acgactatga agtcggtaaa
    14341 cgtcacgccc tgccgatgat caacatcctg acctttgacg gcgatatccg tgaaagcgcc
    14401 caggtgttcg ataccaaagg taacgaatct gacgtttatt ccagcgagat cccggcagaa
    14461 ttccagaaac tggagcgttt tgctgcacgt aaagcagtcg ttgctgcggt tgacgcactc
    14521 ggcctgctgg aagaaattaa accgcacgac ctgaccgttc cttacggcga ccgtggcggc
    14581 gtagttatcg aaccaatgct gaccgaccag tggtacgtgc gtgccgacgt gctggcaaaa
    14641 ccggcggttg aagcggttga gaacggcgac attcagttcg taccgaagca gtacgaaaac
    14701 atgtacttct cctggatgcg cgatattcag gactggtgta tctctcgtca gctgtggtgg
    14761 ggtcaccgta tccctgcatg gtacgacgaa gcaggtaacg tttatgttgg ccgcaacgaa
    14821 gaagaagtgc gcaaggaaaa taacctcggt gccgacgttg ccctgcgtca ggacgaagac
    14881 gttctcgaca cctggttctc ttctgcgctg tggaccttct ctacccttgg ctggccggaa
    14941 aacaccgacg ccctgcgtca gttccaccca accagcgtga tggtatccgg cttcgacatc
    15001 atcttcttct ggattgcccg catgatcatg atgaccatgc acttcatcaa agatgaaaat
    15061 ggcaaaccgc aggtgccgtt ccacactgtt tacatgaccg gtctgattcg tgacgacgaa
    15121 ggccagaaaa tgtccaaatc caagggtaac gttatcgacc cgctggatat ggttgacggt
    15181 atttcgctgc cagaactgct ggaaaaacgt accggcaata tgatgcagcc gcagctggcg
    15241 gacaaaatcc gtaagcgcac cgagaaacag ttcccgaacg gcattgagcc gcacggcacc
    15301 gatgccctgc gcttcaccct ggcggcgctg gcgtctaccg gtcgtgacat caactgggat
    15361 atgaagcgcc tggaaggtta ccgtaacttc tgtaacaagc tgtggaacgc cagccgcttt
    15421 gtgctgatga acacagaagg tcaggattgc ggcttcaacg gcggcgaaat gacgctgtcg
    15481 ctggcggacc gctggattct ggcggagttc aaccagacca tcaaagcgta ccgcgaagcg
    15541 ctggacagct tccgcttcga tatcgccgca ggcattctgt atgagttcac ctggaaccag
    15601 ttctgtgact ggtatctcga gctgaccaag ccggtaatga acggtggaac cgaagccgaa
    15661 ctgcgcggta ctcgccatac gctggtgact gtactggaag gtctgctgcg cctcgcgcat
    15721 ccgatcattc cgttcatcac cgaaaccatc tggcagcgtg tgaaagtact ttgcggtatc
    15781 actgccgaca ccatcatgct gcagccgttc ccgcagtatg atgcgtctca ggttgatgaa
    15841 gcggcgctgg ccgacaccga gtggctgaaa caggcgatcg ttgcggtacg taacatccgt
    15901 gcagaaatga acatcgcccc gggcaaaccg ctggagctgc tgctgcgtgg ttgcagcgcg
    15961 gatgcagaac gtcgcgtaaa tgaaaaccgt ggcttcctgc aaaccctggc gcgtctggaa
    16021 agcatcaccg tgctgcctgc cgatgacaaa ggtccggttt ccgttacgaa gatcatcgac
    16081 ggcgcagagc tgctgatccc gatggctggc ctcatcaaca aagaagatga gctggcgcgt
    16141 ctggcgaaag aagtggcgaa gatcgaaggc gaaatcagcc gtatcgagaa caagctggcg
    16201 aatgaaggct ttgtcgcccg cgcaccggaa gcggtcatcg cgaaagagcg tgagaagctg
    16261 gaaggctatg cggaagcgaa agcgaagctg attgaacagc aggctgttat cgccgcgctg
    16321 taatcaaaac acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt
    16381 acagaaatgg taaagaaggc ataattcccc gtgaaatact ggctaagaag cctttatctg
    16441 gttgttcttc tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt
    16501 ttgcctgcca gtcaatcatc catattttca gaagtggata agccagaccg cccgtaatcc
    16561 cggatatcac caccaacgca cacatgcgat aaagcatacc gtggtatgtt aaagttgagc
    16621 gaaaacgaat acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca
    16681 ccgttaaata actggtgcta acagcaatcc caaaataata gattaactgc gcgattatca
    16741 ttttacgttg catttccata aattgctgta aattctcaat atcatcattt gcatacacag
    16801 aactatcata cgcatttaat atttgatcga agataatata accagcaaca gcgagaaaag
    16861 gtaataaagc taaaaatgcg agaatggcat attttataca atacgtagta tctaatttca
    16921 ccttgaaacg atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat
    16981 ataaagtacc attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag
    17041 atataataac actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag
    17101 tccccatccc aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca
    17161 aagagcaatt aaaactaaat cttacgccgt tgagactggt catcaaggcc tgataacgca
    17221 gtccttttgc tgccataaat ataagcaaaa ccaacagtga caaagtcaaa acacagccaa
    17281 caagcggcat atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa
    17341 gacaactaaa aaaaacattc ccaccagtaa ttccataaga aaatcgttgt ccgttaactt
    17401 ccatattagc atagagataa cgcctacatt tcattaatgc ccatggtaaa tagataccta
    17461 gcgttataat cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtgc
    17521 catgaaaaac aaacgaatgg gacggaacat ccatttcatt aataacttga gccataaaaa
    17581 ttccctgaat taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc
    17641 cacgttacaa tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc
    17701 ttgcgcacgt aagaagagag tggtattaaa caaatgtata attactttat ttttattggg
    17761 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga
    17821 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
    17881 taaaggctac accgtcgccg atccgaatct tgacgaactg tatcaagtgt atagtcagcc
    17941 gggtcatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
    18001 gccattagca ggtagtgaat cggacatttg cgaactgcaa aagatgtatt ttcttccggc
    18061 tattcgcggt aaagggctgg cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
    18121 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
    18181 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
    18241 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtgtg tgctatcggg
    18301 cattttaatc aattaattag tggcgaacac cgtcatcgtc ttcatcgaca aaatcttcat
    18361 cgtcgccgtc ttcgccattg ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
    18421 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
    18481 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
    18541 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgctgct ttttccaggg
    18601 tttccagatc gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc
    18661 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
    18721 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcatatt gtcacacacc
    18781 actggcattg cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat
    18841 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg atctatgtcc
    18901 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
    18961 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggcaaaga agaagccaaa
    19021 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
    19081 ttcgaagttg ccgcatatga ccagggtgct cgcgttactt atctcggccc aagcggcagt
    19141 cagattggtc ataaagagtc gattaaagac actgcccgcg tgctgggtcg catgtatgac
    19201 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
    19261 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc
    19321 ctcaccatgc aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca
    19381 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag cggcggcgct taccggtctg
    19441 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
    19501 cgcgctctgg cacagcaaaa tggcgggaat attacgctaa cggaagatgt agccaaggga
    19561 gtagaaggtg ctgactttat ctataccgat gtgtgggtgt cgatggggga agcaaaagaa
    19621 aaatgggctg aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag
    19681 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgttcca tgatgaccaa
    19741 acgacgcttg gcaagaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
    19801 gatgaggtct tcgaatctgc cgccagcatt gtttttgatc aggcggaaaa ccgcatgcat
    19861 accatcaaag cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
    19921 gatgccggga aatacaaaaa aatcccggca tcacattatt ttatgcagtg atagtttttt
    19981 tattccagcc aaatttttct att
//
LOCUS       DAPGRN010000089        19840 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPGRN010000089 DAPGRN010000000
VERSION     DAPGRN010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.089"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            224..724
                     /gene="tssB"
                     /locus_tag="RHW33_003339"
     CDS             224..724
                     /gene="tssB"
                     /locus_tag="RHW33_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV1646814.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            759..983
                     /locus_tag="RHW33_003340"
     CDS             759..983
                     /locus_tag="RHW33_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1646815.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1034..2509
                     /gene="tssC"
                     /locus_tag="RHW33_003341"
     CDS             1034..2509
                     /gene="tssC"
                     /locus_tag="RHW33_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV1646816.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2516..2929
                     /gene="tssE"
                     /locus_tag="RHW33_003342"
     CDS             2516..2929
                     /gene="tssE"
                     /locus_tag="RHW33_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV1646817.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNSSQHYRVD"
     gene            2933..4783
                     /gene="tssF"
                     /locus_tag="RHW33_003343"
     CDS             2933..4783
                     /gene="tssF"
                     /locus_tag="RHW33_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HDV1646818.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKAEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRLWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4747..5829
                     /gene="tssG"
                     /locus_tag="RHW33_003344"
     CDS             4747..5829
                     /gene="tssG"
                     /locus_tag="RHW33_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HDV1646819.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALIRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5854..7134
                     /gene="tagH"
                     /locus_tag="RHW33_003345"
     CDS             5854..7134
                     /gene="tagH"
                     /locus_tag="RHW33_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HDV1646820.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDHNDDISAAEMAQRHLAVTPLLRGLGGSLTVSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7131..7655
                     /gene="tssJ"
                     /locus_tag="RHW33_003346"
     CDS             7131..7655
                     /gene="tssJ"
                     /locus_tag="RHW33_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HDV1646821.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYMTDDSKLQAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7658..8989
                     /gene="tssK"
                     /locus_tag="RHW33_003347"
     CDS             7658..8989
                     /gene="tssK"
                     /locus_tag="RHW33_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HDV1646822.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVTSDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSSQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADVH
                     DSELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8994..9755
                     /gene="icmH"
                     /locus_tag="RHW33_003348"
     CDS             8994..9755
                     /gene="icmH"
                     /locus_tag="RHW33_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HDV1646823.1"
                     /translation="MDEGSLSLPPFTGYDEKSLRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAVQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9764..12523
                     /gene="tssH"
                     /locus_tag="RHW33_003349"
     CDS             9764..12523
                     /gene="tssH"
                     /locus_tag="RHW33_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HDV1646824.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGLVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPSAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESEQDNTGAVPADEAGSVQPEETAETVSPVQRLAQLTAELDAL
                     HNNRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLAIA
                     SLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSE
                     FQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAF
                     DKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALL
                     ARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRAEN
                     GARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESVTK
                     DETVL"
     gene            12520..13263
                     /gene="tagO"
                     /locus_tag="RHW33_003350"
     CDS             12520..13263
                     /gene="tagO"
                     /locus_tag="RHW33_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HDV1646825.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVTTTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13268..14680
                     /gene="tssA"
                     /locus_tag="RHW33_003351"
     CDS             13268..14680
                     /gene="tssA"
                     /locus_tag="RHW33_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HDV1646826.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPDWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWSVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELALATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIA
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQSITSTPQAESDGRTPLAA
                     VSADMVADYQSRLASADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADTIR
                     DEVIRFLERLPQLTGLLFNDRTPFLSEQTKQWLAASPDGKVAPVAQIGEESQAARACF
                     AGQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLIQLAQQQYRMLLMIGSQMM
                     VSDWEPSLLTQLEQKFTAEQ"
     gene            14789..18223
                     /gene="tssM"
                     /locus_tag="RHW33_003352"
     CDS             14789..18223
                     /gene="tssM"
                     /locus_tag="RHW33_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HDV1646827.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGTEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLMRSRRRLTVFSTTGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLVGASGGAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18234..19583
                     /locus_tag="RHW33_003353"
     CDS             18234..19583
                     /locus_tag="RHW33_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HDV1646828.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQPSSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQNSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYQDARAQKQNTDALEKQINERLEGVLSRWLLLNNNTIPTIKKGIEFQ"
     gene            19610..>19840
                     /gene="hcp"
                     /locus_tag="RHW33_003354"
     CDS             19610..>19840
                     /gene="hcp"
                     /locus_tag="RHW33_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDV1646829.1"
                     /translation="MANLIYLTLNGDNQGLISSGCSSQPSIGNKAQTAHIDQIMIYEF
                     MHGLNRDQNVNHHHLTIKKPIDKASPLLGKAIC"
BASE COUNT         4643 a         5469 c         5507 g         4221 t
ORIGIN      
        1 gcgaattgca caggttcata caatctattt aacgcttgca gaatacccct ctttacgatc
       61 gataaaaagt ccttttatta tttattttga agcgtaaaat gcggggtgat gaaggagtgg
      121 tgattttatt ttcaccgcca tcaaggcggc atttccgata ttttttgcgg tacgaatatg
      181 ccgtgttcat ggcctgtacg agataacatg acggatcact tttatgagca aaaaatttga
      241 aggcagcgtc gcgcctcgcg agcgtattaa tatatcgtat gtcccaaaaa cagatggcca
      301 gacggctgaa gtggaacttc cgcttaacat gctcgttgtc ggcgataccg gaaatacgca
      361 ggaaacatcc tcactggatg aacgtcaggc agtttccgtt aacaagcaca atttcggtgc
      421 agtgatggct gaagcagcca ttggcctcaa ttttaccgtc cccgccacgc tgaagggaag
      481 cacaacggac gatgagctga atgtggcgct gaatatcaag tcactggatg atttttcacc
      541 agatagcgtg gcccggcagg ttccggaagt gaataaattg ctggaactgc gtgaagcact
      601 gactgcactg aaggggccga tgggtaacct tccggcattc cgtacccagc tccaggcatt
      661 gctggaaaat gaagagtccc gcgagcagtt gctcaaagaa atcggccagg tgagcaataa
      721 ataaccacca ctatttcatt aaacagggtt atccaggcat gacggcacac aaaaatatct
      781 ccgggacata ccacctctct gttgcagaca ttctgcaggt ggtttaccag gtatgtttca
      841 gcccgtccgt tgaaatcaac caggatggtg tggctgcgct gattacaaca ctggatcgcc
      901 gtatcagtga tctattggat gagattatcc atttttgtga gtttcagcag agcgcgtcgc
      961 actggcagcg cgtacttcac tgacggcgaa aagccggttt cagaaacaca gctaaaacag
     1021 aaggattttc agtatgtctt tacaggaaga ggaactcgtt tccagtcatg ccgggcagcc
     1081 tgagcaggca tcgtcgctgc ttgatcaaat catggcacag acccgtattc agcccggttc
     1141 ggaaggttat gacgttgccc gccagggtgt gacagccttt attgccagca tcctgcaatc
     1201 cactgcatct gccgaaccgg tcaataaact ggccgtggac agcatgattg cagatattga
     1261 tgaacgcatc agccgccaga tggatgtcat catccatgcg cctgcctttc agcaggtcga
     1321 gtcgttctgg cgctcactga aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa
     1381 ggtcaacgtg cttcatgtca ccaagcagga acttctggaa gactttgagt ttgcgccaga
     1441 aattattcag tccggatttt ataagcacgt ttattcgtcc ggtttcggac agtttggcgg
     1501 tgaacccatc gctgccgtgc tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa
     1561 actgctgcaa tacgtcagtg ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt
     1621 ttccccggag tttatggggc tgaactcgtg gaccgaactg cctaatatca aagatcttta
     1681 tgccatcttt gaaggtccgg cctacaccaa atggcgcgcc ctgcgtgact cggaagattc
     1741 gcgctattta gggcttacgg caccccgctt cctgcttcgc cagccctatt caccaacaga
     1801 taaccctgtt aagaacttta actattacga agatgtcagc cagaaccacg aagattatct
     1861 gtggggtaat acggcctgga tgctggcatg caatatcgca gacagttttg ccaaataccg
     1921 ctggtgtcca aacattatcg gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca
     1981 tctgttcgaa acgatggggc agattcaggc caaaatccca accgaggtgc tggtcaccga
     2041 tcgccgcgaa tttgaactgg ctgaagaggg ttttatcacc cttaccatgc gtaaagactc
     2101 tgataatgca gcctttttct ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa
     2161 agatgcagaa accaactata agctgggtac gcagcttcct tatctcttca tcatcaatcg
     2221 gttagcgcac tacatcaagg tgttgcagcg tgaacaactg gggtcatgga aagaaaggag
     2281 cgacttagaa agagagctta atacctggat ccgtcagtat gttgccgacc aggaaaaccc
     2341 gcctgcagac gtgcgcagcc gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt
     2401 agaaggcgaa cccggctggt accaggttgc gctaagcgtg agacctcatt ttaaatttat
     2461 gggggcaaat tttgagcttt ccctggttgg ccggttagac agggagtaac acaccatgat
     2521 accggatcgg gaaagaacca caggttctct ttttgagcgt atggaagcct cttcggccag
     2581 aaaccggcag ggaggcagta ttcattcgct caggcaatcc attcgccaga acctacgaaa
     2641 cattcttaat acccggtccg gtagttgccg gggtgcgcct gagctgggca ttgatgaacc
     2701 ggaaggggct gaaaacttcc gcgaatccat gagtcgggcc atcgagcaat gtatagaacg
     2761 ctatgaaccc cggatctcac acgctgaagt gcaggcagtg gtttcatccg cctccagccc
     2821 gctggatatg acgtttcata ttacagcctg ggtgacattt aacgaaacgc atgaggtgct
     2881 tgaatttgat atggcaccga atagcagcca gcattaccgg gtggactgac aaatggaatt
     2941 tgaagaacgc tattttcggg aagaactcga ttacctgcgc cagcttagca agctgctggc
     3001 aacggaaaaa ccccatctgg cccgcttcct ggccgaaaaa gatgcggatc cggatattga
     3061 acgcctgctg gaaggggtgg cttttcttac cggcaatctc cgccagaaaa ttgaggatga
     3121 attcccagaa ctgacgcacg ggcttattaa gatgctatgg cctaattacc tgcgtccggt
     3181 tccggcaatg acccttattg aatatacgcc ggatatggat aagtcttctg taccggtgtt
     3241 aatcccccgt aatgagcagt ttacaaccaa cgccggggaa atcagagttg atgaagtgct
     3301 gccctctgat gctaaaaagg ctgagccgcc tccctgtacc ttcaccctct gccgggatat
     3361 ctggctgctg cccgttcgcc tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt
     3421 tatcaacatc accttttcgg tcgcaccggg aacggacttc cgcacgctgg atctgaacaa
     3481 acttcgcctc tggctcggca atgacgacaa ctatacccgt gaccagcttt atttatggtt
     3541 ctgcgaatac ttgcagggtg ccgacctgac tgtgggtgaa cagcatattc gcctgcctga
     3601 gtttatgcta aaagctgtcg gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa
     3661 cgtccacagc ggctaccgga tccttcagga gtatttctgt tacccggatg cgtttctctt
     3721 ttttgatctt tgcggttgtc cggcgttgcc tgacggattg caggctgaat cctttaccct
     3781 gcaactgcgt ttttcgcgcc ctttacccgt ggacatccgg ctgcgccgcg attccctgcg
     3841 cctgtattgc gcacctgcca ttaatttatt tatccaccat gcagaagcca tcacgctgga
     3901 caaccggcgg gcagactatc cgctggttcc cagccgccat tacccacaac attacgatgt
     3961 attttccgtt aacagtgtgg tgagccaggt ccaggatatg ttcaggaaaa aagatctggg
     4021 gcgtcctgtt tcgacgcagg ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca
     4081 gatggaatac agccggaagc gggaagtggt gtactggcat caccggacca aaacatccct
     4141 gttccatcgc ggctttgatc atacccttgc ctttatacat gctgatggca gttatccgtc
     4201 agacgaatct ctgctcagta atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga
     4261 gcttccgtca caaattcgtt ccggcgatat caccggcaca accggtaaaa atgcggctgt
     4321 tgcttcattt cgcaacatta cccgcccgac gcaaccactc tggccggtca ttgatggcag
     4381 cctgcattgg tccctactct ccgccatgaa cctgaattat ctgtcattac tggatacgga
     4441 cgcgctgaag caggtcatcg ccaactttga tcgccacgca atccatcatc cgcagacggc
     4501 acggctgtca caacaaaagc tggatgccat tgagcgtctg gagacccgcc ccgttgatcg
     4561 cctgtttacg ggtattcccg tccgggggct ggcctccacg ctgtatctgc acccggagcc
     4621 gtttgtctgt gaaggggaaa tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct
     4681 gtacgccagc gttaactcat tccacatgct gaccgttgtg aacacagaaa gccaagagac
     4741 atggaaatgg acggaaagaa tcgggcagca tcctcttatc tgagtccggg aaatcccccg
     4801 gcagataaag agcagaatga tccgctggca caggttttcc ataatgcctg ctcttacaat
     4861 ttttttgcga tggcggagct gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa
     4921 ttatccctgc gcgacgatcc ggcacaggaa accctgcgtt tcagcgccga tgccagcctt
     4981 gccttcccgt gcagtgatat cagtgcgctg atacgggata cgtcaggggc attcaggatg
     5041 accaccacct ttatggggtt acaggggagc cagtcgcctc tccccggtta ttacctcgat
     5101 cacctggcct ggaaagcggt acatgaacaa agtccggtag gtgattttct ggatatgttc
     5161 agccaccgcc tgacccagtt tgtctggcat atctggcgta agtaccgcta tcacatcagt
     5221 tttcgtaacg gcggtgtgga tgcgttctcg cagcgcatgt actcccttgt cgggctgggc
     5281 caccgccagc ttcgcgataa gctggcgatt aatcacagca aaatgctggc gtattccggg
     5341 atcctggcaa atccgggacg atccccggaa attatctgtg gcctggtgtc ccactgcttt
     5401 gatttgtcag aagtgacgct gcaaaactgg caacgtcgca aggtggatat tgaaccggat
     5461 cagcaaaata gtctggggag ctattctctt aaaaatggcg aaaaactggc gggaagatcg
     5521 gtactgggca actttgttct gggtacacga gtgcccgatc tcagcggaaa attccagctc
     5581 agtattacca gcctgacccg aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc
     5641 ctgccactga cgatgttcgt gtccttcatc ctgcgcgatc agcttgcctg ggacttacat
     5701 ctggggctgg cgccggagca ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg
     5761 ctgggctgga ccagcttcct cggcacaccg gaagaacgac cctcagtcac catcagggta
     5821 cggtcgtaaa ttatgataat cacggagcag aatatgccgg aagagaaact acagacgctt
     5881 tcattgcagg tcattaacgg cagtgagctg gaaagcgggc gggcggcgcg ctgtctgttc
     5941 acacagcagg gaaatgtcgg ccatggcccc gaatgccact ggtcggtaca ggatcgtcag
     6001 cagagcattc cggcccaggc ttttaccgtt atcctgcacg atggcacatt ttgtctacgc
     6061 ccgcagacgg cacaactgtg gctgaatcag gcaaaagtca cagcaacatc agacctgata
     6121 cagttgcgcc agggcgatga gatccagatc ggacggctga tggtgagggt tcatctgaac
     6181 cggggagata ttccccatta cgatgaggaa atggccactc ccgaaaccat cgttaccaat
     6241 cgcgatatgc tcacggatac cctgctatca acggagggtg cgccacacta tccgggaatg
     6301 actcaccggc accagcttgc agacaccgtg gtaaacggtt tttctgccga tccactccag
     6361 gcacttcagt ccgaaagcct gattaccacg ggcgatccgc tttcaggcat tgcggctgtc
     6421 cggccatcgg caccgctgtc cgatccggca agtaatgggg ggatcaatac tccgtttatg
     6481 gatctgccgc ccatttatgc cagccctggc gatcataatg atgacatctc tgcggcagaa
     6541 atggcgcaac gccaccttgc ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc
     6601 gtgagcaatt ccgacgatgc ggatgatttt ctggaggagg ccggacgaac gttacaggcc
     6661 gcaataaaag gtctgctcga tttgcagcag cagcgtaaca gcctctcaga caaacatttg
     6721 cgcccgctgg aagataaccc gctgcgcctg aacatggatt acgccaccgc gctcgacgtg
     6781 atgtttgccg aaggtaaaag cccggtacat ctggcggctc ccgccgccgt cagtgaaagc
     6841 ctgcgcaatg tccgccacca cgaagaagct aaccgggcgg cgattgtgga gtcgcttcgt
     6901 gtcctgctgg acgccttctc accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc
     6961 agccatgaac tgcgccagcc gctggatgat gccggagcat ggcaaatgta cagccattat
     7021 tacgaagaac tggcctccga tcgccagcag gggtttgaga tgctgtttaa cgaggtctac
     7081 gcccaggtct atgaccgggt gcttcgtgaa aaacagcggg agccggaagc atgaacagga
     7141 aggcgtttct ggcctgcgtg ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa
     7201 aaatcagcga ggtgatcaaa aacccggata tccaggtcgg gagcctgaag gaacaacctt
     7261 cggaaataac cgtcaccctg ctgactgaac cggacaccaa taccaatgcg gagggtgaat
     7321 cagccgccgt ggatgtgcaa ctggtgtaca tgactgacga ctcaaaattg caggcagccg
     7381 actatgacca gatagccagt acgccgctac cggatgtgct gggtaaaaac tatatcgacc
     7441 accaggattt caacctgttg ccggacacca tcaaaaccct gccgccggta aagctggatg
     7501 agaaaacgca gtttatcggt gtcgtggcct atttctccga tgaccaggcc actgaatgga
     7561 aacaaatcga gacggtggaa ggaaccggcc accattaccg cctgctggtg catgtacgtc
     7621 aaagcagcat cgaaatgaaa aaagaggatg aataattatg gcaaccaccc gcaacaaggt
     7681 gatgtggcag gaagggatgc tgatgcgccc acaccatttc cagcaacagc agcgttacaa
     7741 cgactacctg gataaccagc gtttccgggc catgaatgat ttatcctggg gatttaccga
     7801 actcaccctc aacaatgaac tgctggcgca gggtaagatc atgattgaca gcgcgtcagg
     7861 cacactgcct gacggcaccg tcttttctat ccccgaccag gacgcactgc ccgatccgct
     7921 gcacccgcaa aattttccgg acgaaagaag ccgcaatatt tacctcgctc tgccggtcac
     7981 cagtgatgtg agaaatgaaa tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta
     8041 tgccgatgtc cgggatctgc attcagaaga aggcgacgcg cgaacactga cgctgggaca
     8101 actgacgccg cgcattatga gcggtgcaga agatatgagt gcctatatta cgctcccgct
     8161 ttgccgtatc agcgatcgcc atgccgacgg ctccctgacg ctggatgacg attttatccc
     8221 ctccagccag aatattcagg tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg
     8281 ggccattggc ggacgggcaa gcgatctggc aaaccgcatt ggctctccgg cgcagagcgg
     8341 catcgcggat gtggcggaat ttatgatgtt gcagttgctt aaccgtaacc agacccggtt
     8401 tacccatcgc gcccgccgat cccagctcca cccggaagat ttctaccttg atcttgccgg
     8461 gttgctgggt gaactgatga cctttacaga gccgtcacgc ctgccctgcc cgcttgatgt
     8521 gtatgatcat catgacctga ccaaaacatt taaaacactg ttaccggaag tcaaacgggc
     8581 gctgcatacc gtactgtcgc caagagcggt caatctgccg ctgcatctgc gtgacggcat
     8641 ctggcaggcc gatgttcatg actcggaact gctacagtct gccacctttg tactggccgt
     8701 ggcagcaaat atgcctgtcg atcagatcca gcgtcagttt atccagcagt cgaaaatttc
     8761 ctcgccggaa aaaatccgca atatggtcag tgtgcagatc ccgggtattc ctctgcgcgc
     8821 actgatggtg gccccccgcc agcttcccta ccattccggg ttcagctatt tcgaactcga
     8881 caagagcggc caggcctgga cagaaatggc tgccgccggg gccgttgcac tgcatgtatc
     8941 cggcagtttc ccggatctga acatgcaact gtgggcgata agagggtaag acgatggatg
     9001 aggggtcact gtctctgcca ccatttaccg gatacgacga aaaaagcctg cgcgactacc
     9061 atctcgcgct tcgtggcaac agcctgaatc cgatgatcga cgcggcgacg ccgctactcg
     9121 gtatggtaat gcgcctttcc accatgaaca gccagacgat gccggagcat ctttttgctc
     9181 aggtggtgac agatgttcag gccgtggagc aactgcttca ggaacagggc tacgaaccgg
     9241 gcgtgattat ctcgtttcgc tacatcctct gcacgtttat tgatgaagcg gcgctcggta
     9301 acggctggtc gaacaagaac gagtggatca agcagtcatt gctggtccat ttccacaacg
     9361 aagcctgggg tggcgagaaa gttttcatcc tgctggagcg cctgatccgt gaacccaaac
     9421 gttaccagga tctgctggaa tttctctggc tctgtttttc tctgggattt cgcgggcgct
     9481 acaaagtggc agtacaggat cagggggaat ttgagcagat ctaccgccgt ctgtaccacg
     9541 ttctgcataa actgcgcggc gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc
     9601 agggcgggcg ttatcagctc atcagtcgcc tgactgttaa acatattttt tgtggtggcg
     9661 ttgtcgtgct ggcgttgttt tacctgttct atctgttgcg gctggacagc cagacgcagg
     9721 acatcctgca ccagctaaac aaattacttc gataaggacg tttatgatcc agattgatct
     9781 tcccacgctg gtaaaacggc tgaacctgtt ctcccgccag gcgctggaga tggccgcctc
     9841 tgaatgtatg agtcagcagg ctgcggaaat taccgtcagc catgtgctta ttcagatgct
     9901 cgccatgcca cgcagtgacc tgcgggttat tacccgccag ggcgatattg gcatggaaga
     9961 gttgcgccag gcgttgacgg tagagaacta cacaaccgcc cgttctgcgg acagctaccc
    10021 ggcgttttcc ccgatgctgg ttgagtggct taaagagggc tggctgctgg cgtcggctga
    10081 gatgcagcac agcgaactgc gcggcggcgt gttgctgctg gccttgctgc attcgccgct
    10141 gcgttatata ccgcctgctg ccgcccggct gttgaccggc attaaccgtg accgtctgca
    10201 acaggacttt gtgcagtgga cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg
    10261 taaaggcgca ggcacactga cggacgcctc tgacaccctg cttgcccgct acgccaaaaa
    10321 catgaccgca gacgcccgta acggcaggct agacccagta ctgtgccgcg accacgaaat
    10381 cgacctgatg atcgacattc tctgccgccg ccgtaaaaat aacccggtgg tggtgggcga
    10441 agcaggcgtg ggtaaaagcg cgctgattga agggctggcg ctgcgcatcg tggcaggcct
    10501 ggtgccggac aaactgaaaa acaccgatat catgaccctt gacctgggcg cattgcaggc
    10561 cggggcgtcg gtgaagggtg aattcgaaaa acgcttcaag gggctgatgg cggaggtcat
    10621 ttcctctccg gtgccggtca ttctgtttat cgacgaagca cataccctga ttggcgcggg
    10681 caaccagcag ggcgggctgg atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga
    10741 gctgaaaacc atcgccgcca ccacctggag cgagtacaaa aaatacttcg agaaagacgc
    10801 cgccctgtcg cgccgcttcc agttggtgaa ggtcagcgaa cccagtgccg ccgaagccac
    10861 cattattctg cgcggtctgt cggcggtcta tgaacagtct cacggcgtcc tgattgatga
    10921 tgacgccttg caggccgctg cgacattaag tgaacgttat ctctccgggc gtcagttgcc
    10981 ggacaaagcg attgatgtgc tggataccgc ctgcgcccgt gtggcaatca acctgtcatc
    11041 gccaccgaaa caaatctcgg cgctgaccac cctgagccac cagcaggagg cggaaattcg
    11101 ccagcttgag cgcgagcttc gcatcggact gcgtaccgac acatcacgga tgaccgaggt
    11161 gctggtgcag tatgatgaaa cgctgacggc gctggatgaa ctggaagcgg cctggcacca
    11221 gcagcagacg ctggtccggg agattatcgc gctgcgccag cagttactgg gcgtggcaga
    11281 ggacgatgcg gcgccgttgc cggacgcaga taccgtggag gatacgcagc cagagtcaga
    11341 acaggataat accggtgctg taccggctga tgaagctggc agcgtacagc cggaagagac
    11401 cgctgaaaca gtttccccgg tacagcggct ggcccagctc actgccgagc tggacgccct
    11461 gcataacaac cggttgctgg tctccccgca cgtcgataaa aaacagattg cggcggtgat
    11521 tgccgaatgg accggcgtgc cgctcaaccg cctgtcgcag aatgaaatgt cggtcatcac
    11581 cgacctgccg aaatggctgg gcgacaccat caaaggccag gacctggcga ttgccagcct
    11641 gcataagcat ctactgaccg cacgcgccga cctgcgtcgt ccgggacgcc cgctcggtgc
    11701 gttcctgctg gctggcccca gcggtgtggg taaaaccgaa accgtcctgc aactggcaga
    11761 gctgctctac ggcggtcgcc agtacctgac caccatcaat atgtccgaat tccaggagaa
    11821 acacaccgtc tcgcggctga ttggttcgcc tccgggctac gttggctacg gcgaaggcgg
    11881 cgtactgacc gaagcgattc gccagaaacc ctactcggta gtactgctcg atgaagtgga
    11941 aaaagcgcac ccggatgtgc tcaacctgtt ctaccaggcg ttcgataagg gcgaaatggc
    12001 agacggtgaa ggccgcctga ttgactgcaa aaatatcgtc ttcttcctga cgtccaacct
    12061 cggctaccag gtaatagtcg agcatgccga tgacccggaa accatgcagg aagtactgta
    12121 tccggtgctg gccgacttct tcaaacctgc cctgctggcg cgtatggaag tggtgccgta
    12181 tctgccgctg tcgaaagaga cgctcgccac cattatcgcc gggaaactgg cccgtctgga
    12241 taacgtgctg cgcagtcgct ttggtgcaga agtggtcatt gaaccggaag tgacggacga
    12301 aatcatgagc cgcgtcaccc gcgcggaaaa cggcgcgagg atgctggaat cggtcatcga
    12361 tggcgacatg ctaccgccgc tctcgctgct gctgttgcag aaaatggcgg ctaacacggc
    12421 gattgcccgg attcggttgt cggcagtgga cggcgcattt acggcagacg tggaagatgc
    12481 tcagaacgac gagtccgtca caaaggatga aacggtttta tgagcaggtt gcgcgttttc
    12541 cggcagacag gatggtttat cgcgggcctg atgacgggct tacccgccac tgccgcgccc
    12601 gcagaagcat cgtcaatggc cggtgtggcg gtcgccgtcg ccaccacgac cccgccggat
    12661 gcgacagcca cgttacaggc catgcagtcc tgtcggcggg agtccgcggc gctggagcgg
    12721 cttgactgct atgaccgcct gctggcaccg ctgtccccgt cagggtttga cggggcgctg
    12781 gtcaaagccg gttttgtggg cgaggcctgg acccgcgcca cagagcagga aaaacgccgc
    12841 gagggcaaca ccacagaact gctggtgacg caggtgccgg gggagcgccc gacggtggtg
    12901 acaaccaccc cggctatcgg ccatgttccg ccgcgcccgg tgctgatgtt cagttgcgtg
    12961 gacaatatca cccggatgca ggtggcgctg atgcaccctc tggatgtcca tgatatcgcc
    13021 gtcactctga atgccgacag ccgggcattg cgcagccact ggtttgtgcg cgagaacggc
    13081 accctgctgg agtccagcag ggggctgtcc gggattgatg aaattaagca actgttcggc
    13141 gcaaaaacgc tgacagtgga taccggcgca gataacgccg caggaaagct gacctttaac
    13201 attgacgggc tggcaagggc gattgccccc ctgcgcgatg cctgccactg ggcgggagaa
    13261 taaaaatatg gcaatggact tacgcgatcc gaatgtctgg atatcgcacc tgctggaaaa
    13321 cctgccggaa gaaaaactgg catcggcgct gaaagatgac aacccggact gggagtatat
    13381 cgacggcgaa atcgtcaaac tgggatctct ggcccacagt caactggata tcccggaact
    13441 ccagcgccgg gggctggtca ttctggcttc ggagagtaag gactttcgtc tgctggcgca
    13501 tctgctgaga accctgcaac acgccggtga tcctcatctc gcattgcgcc tgctggcgct
    13561 gtatgtggag cattactggt cggtggctgc gccgcagaat atggcacata aaaaacgctt
    13621 tgccagccag gtcattaaac gctttgaaac aggtattgaa ggcttttcac agaacgccgc
    13681 cacagcgcag cgcgacgcgc tgtcagggga actggcgaaa ctggcgcagt gctggcagtc
    13741 acataacgcc ccggaactgg cactggctac cgatgatctt tttgccctgt accagcgtgc
    13801 gtttaatcgt gcagcaccgg ctcctgtccc cactccggcg gcctccggta gttcaccaca
    13861 gaccaccgcc acgtctgaaa gcggcgtgac gcaacccagt gctccggctc cacaaatagc
    13921 cattgacagt cacgacgaca aagcctggcg cgacacgctg ttaaaagtgg cggccatttt
    13981 atgtgaacgc cagccggact cgccgcaggg ctatcgcctg cgccgccatg ccctgtggca
    14041 atccatcacc agtacacccc aggcggaaag cgatggacgt accccattgg ctgcggtctc
    14101 tgccgatatg gtggcggatt accagtcccg gcttgccagc gcggatatgg cgctgtggca
    14161 gcaggttgag aaaagcgtat tgctggctcc ttactggctg gacggtcact gtctttctgc
    14221 acagacggca ctgcgtctgg gttacaaaca ggtggcagac accatccgcg atgaggtcat
    14281 ccgcttcctt gagcgtctgc cccagcttac cgggctgctg tttaatgacc gcacaccgtt
    14341 tctcagtgag cagacgaaac aatggctggc tgcttcgccc gacggcaaag ttgcaccggt
    14401 tgcgcaaatc ggtgaggaat cgcaggcagc cagagcctgt tttgctgggc agggtctgga
    14461 ggcggcgctg cgatatctgg acatgctacc cgaaggcgat ccccgcgatc agtttcaccg
    14521 ccagtacctt gccgcacagt tgacggagga ggcggggctg atacagcttg cgcagcaaca
    14581 gtaccggatg ttgttgatga tagggagtca gatgatggtg tctgactggg agccatcatt
    14641 acttacgcag cttgaacaaa aattcacggc agaacaataa aagagaacag gagtttttgt
    14701 gttcagatta ccaacacccc gactactcag cggactcaaa tcagccctac gcccggcgat
    14761 gccccggttt aaagtctccg ctttctggct gctgatactg gcgtggattt ttctgctggt
    14821 atggatctgg tggaaaggcc cgacgtggac gctgtatgaa gaacagtggc tcaaaccact
    14881 ggcgaaccgc tggctggcaa cggcggcatg ggggattatc gccctggtgt ggcttaccgt
    14941 ccgggtgatg aagcgcctgc aacaacttga aaaaatgcaa aagcagcagc gcgaggaggc
    15001 cattgatccg ctcagtgtgg agctgaacgc ccagcagcgt taccttgacc gctggctgct
    15061 gcgcctgcaa cgccatctcg acaaccgccg tttcctgtgg cagttgccgt ggtatatggt
    15121 catcggcccg gcgggcagtg gcaaaacgac actgctgcgc gaggggtttc cgtcagacat
    15181 tatttatgcc ccggagggcg cgcgcggcac agaacaacgc ctgtacctta cgccccatgt
    15241 cggtaaacag gcggttatct ttgatatcga cggcacactc tgcgctcccg ctgatgcgga
    15301 tatcctgcat cgccgtctgt gggaacatgc cctcggctgg ctgaaagaaa agcgcgcgcg
    15361 ccagccgctg aacgggatta ttctgacact cgatttaccc gatctgctta ccgcagacaa
    15421 acgccgccgc gagcatctgt tacagacgct gcgcagccgc ttgcaggata tacgccagca
    15481 tcttcactgc cagttaccgg tttacgtggt actcacccgg cttgatttat tgcagggttt
    15541 tgccgccctg ttccagtcac tgaacagaca ggatcgcgat gcgattctgg gcgtcacgtt
    15601 cacccgccgc gcccatgaaa atgatgactg gcgaacagag ttgaatgctt tctggcagac
    15661 atgggtggat cgaatgaacc tagcgttgcc ggatctgatg gtcgctcaga ctcacacccg
    15721 cgcgtcttta tttagttttt cccgccagat gcagggaagc cgtgaaccgc tggtgtcact
    15781 gcttgagggt ctgcttgatg gcgaaaatat gaacgtgatg ctgcgtggtg tctatctcac
    15841 ctcttcgctt cagcgtggac agatggatga tatattcacc cagtctgccg cccgccagta
    15901 ccggctgggc aataacccac tggcgtcctg gcctctggtg gacaccgcgc cttatttcac
    15961 ccgcagcttg ttcccgcagg cattactcgc agagcctaat ctggcaacgg agagccgcgc
    16021 ctggctgatg cgttcccgtc gccgcctgac ggttttctct accacaggag gcgtggcggc
    16081 actgctgctc atcaccggct ggcatcacta ttacaacggt aactatcagt ccggcatcac
    16141 cgtgcttaag caggccaaag cctttatgga cgtgccgcct ccgcaggggg aagatgacta
    16201 cggcaacctg caactgccgc tgctgaaccc ggtacgcgat gccacactgg cctatggcga
    16261 ctggggcgac cgcagccgtc tggccgatat gggactgtac cagggacgac gtatcgggcc
    16321 ttatgtggaa cagacctatc tgcaactgct ggagcaacgt tacctgccct cgctgtttaa
    16381 cgggctggtt aaagcgatga atgccgcgcc gccggagagt gaagaaaaac tcgcggtgct
    16441 gcgcgtgatg cgaatgctgg aggacaaaag cggacgtaac aatgaggtgg tgaagcagta
    16501 tatggcaaaa cgctggagcg aaaagttcca cggccagcgc gatatccagg cacaactgat
    16561 gtcccatctt gactacgcgc tggctcatac tgactggcac gcagagcgtc aggcgggcga
    16621 cggtgacgcc atcagccgct ggacgccata tgacaagccc gtggtatcag cacagaaaga
    16681 actgagcaaa ctgcctgtct accagcgggt ttaccagagc ctgaaaacgc gggcgctggg
    16741 cgttcttcct gccgacctca atctgcgtga ccaggtaggg ccaacctttg accaggtatt
    16801 tacatctgcc gatgacaaca aactggttgt tccacagttt cttacccgtt acggcctgca
    16861 aagctatttt gtaaaacagc gcgatgaact ggttgaactg acggcgatgg attcctgggt
    16921 acttaacctt acccgcagcg tgaaatacag tgacgccgac cgcgcggaaa tccagcgcca
    16981 gctcaccgag cagtatatca gcgactacac cgccacctgg cgggccggga tggacaatct
    17041 gaatatccgc aattttgagt ccatcggaca actgaccggg gcgctggagc aggttatcag
    17101 cggcgaccag cctttgcagc gggcgctgac cgtgctgcgt gacaacacac agccaggcgt
    17161 cttttctgaa aaactctctg ccaaagaacg ggatgaggcc ctggcagaac cagactacca
    17221 gttactcacc cgtctcgggc atgaattcgc tccggaaaac agtaccctgg cagtacagaa
    17281 agacaaagaa agcacgatgc aggccgtgta tcagcaactc accgagttgc accgctatct
    17341 gctggcaatc cagaacgcgc ctgtaccagg gaaatcggcg ctgaaagccg tgcagttacg
    17401 gcttgatcag aacagcagcg atccgatatt cgccacccgc cagatggcaa aaacgctgcc
    17461 tgctccgctc aaccgctggg ttggcaggct ggctgaccag gcctggcatg tggtgatggt
    17521 ggaagccgtt cactatatgg aagtggactg gcgcgacagc gtggtgaaac cgtttaacga
    17581 gcaactggca aataactatc cgtttaatcc gcgttctgcg caggatgcct cactggatgc
    17641 cttcgaacgc ttctttaaac cggatggcat actggatacc ttctaccagc agaacctgaa
    17701 gctgtttatc gacaatgacc tgagtctgga ggatggcgat aacagcgtca ttattcgcga
    17761 agatattatt gcgcaactga aaaccgcgca gaaaattcgt gacatcttct tcagcaaaca
    17821 gaacggtctg ggaacatcct ttgccgtgga aacggtatcg ctctccggca ataaacgccg
    17881 cagtgtactg aaccttgacg gtcagttagt cgattacagc cagggccgta actataccgc
    17941 ccatctggtc tggcctaaca acatgcgcga aggcaacgaa agcaaactga cgctggttgg
    18001 tgccagcggc ggcgcaccgc gcagtatcag cttcagcggg ccgtgggcgc agttccgcct
    18061 gttcggggcc ggacaactga ccggagtaca ggatggcaac tttaccgtgc gctttagcgt
    18121 ggacggtggc gcgatgacct accgtgtgca taccgacacg gaagataacc cgttcagcgg
    18181 tgggttgttc agccagtttg gtctgtcaga cacactgtac tgaggaggca gggatgaata
    18241 gtaacgtact gacacaaact atcgttaccg gcagtgaccc gcgcgggctg ccggaattca
    18301 gcgccatccg cgaggaaata aacaaagcca gccacccgtc acagcctgag ctgaactgga
    18361 aactggtgga gtcgctggcg ctggcgattt ttaaagccaa cggtgtggat ttacacaccg
    18421 ccacctacta tacgcttgcc cggacacgga cacagggact ggcgggattc tgcgaaggtg
    18481 cggaactgct ggcggcaatg gtaagccacg actgggataa gttctggccg cagggcggcc
    18541 cggcgcgtac tgaaatgctg gactggttta actcccgaac cggcaatatt ctgcgtcagc
    18601 aaatctcctt tgcggaatct gatctgccgc tgatctaccg cacagagcgg gcattgcagc
    18661 ttatctgcga caagcttcag caggtagagc tgaagcgcgt tccgcgcgtg gagaatctgc
    18721 tctattttat gcagaacacg cgtaaacggc ttgaacccca gctgaagagt aacactgaga
    18781 acgccgcaca gaccacggtc agaacgctga tttatgcccc ggaaacacag ccatcttcca
    18841 caccagaagc ggtagtgcct cccctgcccg gcctgcctga gatgaaagtg gaagtgcgca
    18901 gtctgacaga gaatccccca caggccagtg tgataaagca aggcagtacg gtaagagggt
    18961 ttatcgcagg gatcgcctgt tcagtggctg tcgcctcagc attgtggtgg tggcaggtct
    19021 atccggtgca gcagcaactg ttacaggtta acgacaccgc tcagggcgca gcaacggtgt
    19081 ggatggcctc acctgaactc gaaaactatg agcgcaggct gcaacaactt cttgatacct
    19141 ccccggtaca gccgctggaa accgggatgc agatgatgcg tgttgccgac agtcgctggc
    19201 cggaaagcct gcaacagcaa caggcctcga cacaatggaa tgaggcactc aaaacccgcg
    19261 cacagaatag cccgcagttg cgtggctggt tgcagacccg ccaggactta catgcttttg
    19321 cagatctagt gatgcagcgc gagaaagagg gactcaccct ttcctatatc aaaaatgtca
    19381 tctggcaggc ggagcgggga ctggggcagg aaacacccgt tgagtctctg ttgacgcagt
    19441 accaggatgc ccgtgcgcag aagcagaata cagatgcgct ggaaaaacaa attaatgagc
    19501 gactcgaagg cgtgttaagc cgctggctgc tgctgaataa taatacgata ccgacgataa
    19561 aaaaaggcat tgaatttcaa taacattcat gaatacaaag gagttttaaa tggcgaattt
    19621 aatttattta acactaaatg gtgataatca agggctcatt tcaagtggtt gttcatcaca
    19681 accatccatt ggaaataaag ctcaaacagc acacattgat caaattatga tttatgaatt
    19741 catgcatgga ttaaatcgag atcaaaacgt caaccatcat catttgacaa taaaaaaacc
    19801 tatagataaa gcatcaccct tattaggcaa agcaatttgc
//
LOCUS       DAPGRN010000090        19739 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPGRN010000090 DAPGRN010000000
VERSION     DAPGRN010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.090"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(153..1745)
                     /gene="lsrK"
                     /locus_tag="RHW33_003355"
     CDS             complement(153..1745)
                     /gene="lsrK"
                     /locus_tag="RHW33_003355"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV1646830.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECLRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTIRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKVGTPVVVGGGDVQLGCLGLGIVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSERMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1824..2777)
                     /gene="lsrR"
                     /locus_tag="RHW33_003356"
     CDS             complement(1824..2777)
                     /gene="lsrR"
                     /locus_tag="RHW33_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV1646831.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGERL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGERLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3026..4561
                     /gene="lsrA"
                     /locus_tag="RHW33_003357"
     CDS             3026..4561
                     /gene="lsrA"
                     /locus_tag="RHW33_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV1646832.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGSNYTRLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTSVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNDKEINRFSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4555..5583
                     /gene="lsrC"
                     /locus_tag="RHW33_003358"
     CDS             4555..5583
                     /gene="lsrC"
                     /locus_tag="RHW33_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV1646833.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFIAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SALVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5583..6575
                     /gene="lsrD"
                     /locus_tag="RHW33_003359"
     CDS             5583..6575
                     /gene="lsrD"
                     /locus_tag="RHW33_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV1646834.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGAFCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6587..7609
                     /gene="lsrB"
                     /locus_tag="RHW33_003360"
     CDS             6587..7609
                     /gene="lsrB"
                     /locus_tag="RHW33_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV1646835.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGSGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7636..8511
                     /gene="lsrF"
                     /locus_tag="RHW33_003361"
     CDS             7636..8511
                     /gene="lsrF"
                     /locus_tag="RHW33_003361"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV1646836.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNR
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8534..8824
                     /gene="lsrG"
                     /locus_tag="RHW33_003362"
     CDS             8534..8824
                     /gene="lsrG"
                     /locus_tag="RHW33_003362"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV1646837.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            complement(8896..9324)
                     /gene="tnpA"
                     /locus_tag="RHW33_003363"
                     /pseudo
     CDS             complement(8896..9324)
                     /gene="tnpA"
                     /locus_tag="RHW33_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            9332..10540
                     /locus_tag="RHW33_003364"
     CDS             9332..10540
                     /locus_tag="RHW33_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1646838.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLEWAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWH
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFDPVNSFQKNQKKLARLQRQF
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWCGGQVLAVPPAYTSQRC
                     ACCGHTAKENRVSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            10628..11386
                     /gene="tam"
                     /locus_tag="RHW33_003365"
     CDS             10628..11386
                     /gene="tam"
                     /locus_tag="RHW33_003365"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV1646839.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPGARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(11390..12304)
                     /locus_tag="RHW33_003366"
     CDS             complement(11390..12304)
                     /locus_tag="RHW33_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV1646840.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGIENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(12501..13952)
                     /gene="uxaB"
                     /locus_tag="RHW33_003367"
     CDS             complement(12501..13952)
                     /gene="uxaB"
                     /locus_tag="RHW33_003367"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV1646841.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQHLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(14179..15597)
                     /locus_tag="RHW33_003368"
     CDS             complement(14179..15597)
                     /locus_tag="RHW33_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV1646842.1"
                     /translation="MHVHPISTFRVFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(15736..16095)
                     /locus_tag="RHW33_003369"
     CDS             complement(15736..16095)
                     /locus_tag="RHW33_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV1646843.1"
                     /translation="MQSLDTLFARLSRSKFRSRFRLDMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(16095..17021)
                     /gene="glsB"
                     /locus_tag="RHW33_003370"
     CDS             complement(16095..17021)
                     /gene="glsB"
                     /locus_tag="RHW33_003370"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV1646844.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(17085..18473)
                     /gene="sad"
                     /locus_tag="RHW33_003371"
     CDS             complement(17085..18473)
                     /gene="sad"
                     /locus_tag="RHW33_003371"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV1646845.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGADDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNAHNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            18574..19455
                     /locus_tag="RHW33_003372"
     CDS             18574..19455
                     /locus_tag="RHW33_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1646846.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPIIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSTKQGYQ"
     gene            19533..>19739
                     /gene="yneK"
                     /locus_tag="RHW33_003373"
     CDS             19533..>19739
                     /gene="yneK"
                     /locus_tag="RHW33_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV1646847.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICNE
                     EWRTSLNINSATKKIFNNLDNLSYI"
BASE COUNT         4971 a         5014 c         4945 g         4809 t
ORIGIN      
        1 aaatagcaat tttcccttga atatagtact ggtgatgaaa cgatgaatct gcaagctggc
       61 atttaacaag ccagttctaa aagaagggaa ataagaataa ctatacttaa aaactaacag
      121 ccacggtcat catgatgtgg ctgtcaatga aactataacc caggcgcttt ccataacgac
      181 gtcgtcagtc catgatcaac cagccccagc tgatcctgat aaaccgcctg ccacttatcg
      241 cgtgaatcct gataaagttc atgcttttcc gggtctggtg tgtgcgtccg ttcccagcga
      301 accaggcgtt ctccggtttc tgccattgat gaaaaaattc cggcaccgac gccagctgca
      361 atggcacatc ctaatgcagt ggcttctttg accaccggaa tattgacggg taatcccgag
      421 acatcagcga gaatttgact ccataatttc ccttttgaac ctccgcctgc aaagactaac
      481 gatgaaggat gaatattcga aaaatcagca atttgctgca agttacacgc tgatacaatc
      541 gccgcatttt cttccagcgc acggaacaat gtcgctttgt tacatttatc cgggtcaatg
      601 gacaaattaa taaaggaagg cgcagcgtga taccaggttt taaagcgcat tctttcggag
      661 aagatcggca ttacgcccca cgacccaggc ggcacccgac tggccatctc ttccagcagc
      721 gtataggtgt cgatgcctaa acgttcggca atcagttttt cttcggcaca gaaagcatcg
      781 cggaaccagc gcatggtgag tccggtaaaa aagcttatag attcagcttg taccatgcca
      841 ggaataacat gaggattaac gcgcacgttc atttctgggt ctgtcaccgg cgcggctaaa
      901 tttacaactt gctgccagaa tgtgccgcca agaaccgcgg tttgtgccgg acgcacaatg
      961 cctaacccca ggcaaccaag ctgcacgtcg cctcctccaa cgaccaccgg agtgcccacc
     1021 ttcagaccgc agagttccgc cgcttgtgaa cttaccacgc ccagcaatgt gccggtttct
     1081 ttgacaggag aaagaatatc ggcacgcagg ccagccatat ccagcaatgc aggtttccag
     1141 tcacggatgg ttagatcaag aagtcccgtg gtgccagcgt tagagggatc caccgcaagt
     1201 tcgccgctga gcatataggc cagccagtcg ctgatcatgg tgatggttga tgcctgacgg
     1261 taaatatcgg aacgatggtg cgccagccaa agtaatctgg ggatggcact taaagccagt
     1321 gtttgtccgg tcgcgcgata aacttcgttt tcaaaggtat tgttgtgcag ttctttaagt
     1381 tcgctaactt cacgcgccgc tctggcatcc acattggcgc aggcccagat cggtgttcct
     1441 tcattgttat ataaaacaat gccttcacgc atcgaacatg ccgaaacggc agcgatatac
     1501 tccggggcta tgccggcgtt gtgcagcgcc tggcgcaaac actcacacgc cagttgccag
     1561 tttttgttga gatcaaattc catagaacca ggaacgtccg gtactgccag atgccgccac
     1621 tccgcctgtc ccactgctat ttgattgcct tccaggtcga atatcacagc ccgaatactt
     1681 ccggtgcctg catccagagc catcaggtag tactttgatt ctgaaggggt aaagagtcga
     1741 gccattagat tatcctcggc ttatgcgttc tgtctgttcc tctatacgtt ctccatcatt
     1801 cccggtaata aggtcatgca aatttaacta cgtaaaatcg ccgctgctgt gtcctgatcg
     1861 gtaaccagtg cgttgatata accgcctttc attgcagcgg caattgcttc ggctttattt
     1921 tctccccctg ccacgccaac ccggacgggt atggtcttca gcgcgcttaa aggtaagcca
     1981 atcagttcgt tatgtatttt aatgtccgtg acaacgtcac cttttgcatc aaaaaagtag
     2041 cctaaaatgt cgccaaccgc cccttttcgg ccaatcatta accgttcgcc ctggctgata
     2101 taaccggagc gaatgatcgt cgcgtcgtct tgttgactca cggccccaat gccgacaatc
     2161 gccacatccg ctgcttgcgc ggctaacaga acatctttga cgcaattttc attttttagc
     2221 gtacgggcaa tgtcagcgga ggatgcccgc aacggagccg gaataatatt cacactgcac
     2281 gccgcgttaa gctgcccgat acccgtcata taagaaccga cgccaccgga gagcgtgacc
     2341 aggcgaattt gctgtgacga aataaaacca cttaagcgtt gcagcgtatt catggttgcc
     2401 tcgccaaaac caatcgccag catctgttgt ggttgaagta aactcatcaa catatgcgcc
     2461 gcgcctatcc ccagtcgctc gccgacatca gcatccgcaa gcccagggat cacccggaca
     2521 tgttgcagcg aaaactgacg acgtaattgt gtttcatatt ccagacagcc ttcaaagcga
     2581 gaattaatct gcacgcgaat gatgccggac tgatgccctt tctccagtaa tcgcgacact
     2641 ttcaaacggg tcaggccgag acgatcgctg atctcactct gggtcagccc gtcgtggtag
     2701 taaaaccacg cgatccgcgc gacctgttct tcttcacaca ttccctgttc tgaaattacc
     2761 gaatcgttga ttgtcataat tcattcttca ctttgaacat atttaaatct ttaatgcaat
     2821 tgttcagttc ttgcgcattt atatctgtga tggcaaccac agtttgactc tacgagcatg
     2881 aacaaacgca accgtgaaaa tcaaaatagc ataaattgtg atctattcgt cggaaatatg
     2941 tgcaatgtcc acctaaggtt atgaacaaat taaaagcaga aatacatttg ttcaaaactc
     3001 acctgcaaaa ctgaacgggg gaaatatgca aacgagtgat acccgcgcgt taccgctact
     3061 ttgcgcccgc tcggtttata aacagtattc aggggtcaat gtcctgaaag gcatcgattt
     3121 tacgttgcat cagggggagg tccacgccct gctcggcggc aatggtgccg gtaaatcgac
     3181 gttaatgaag attattgccg gtattacccc tgctgatagc ggtacgctgg agattggggg
     3241 cagcaactac accagattaa cgccagttca tgctcatcag ctgggtattt atctcgttcc
     3301 ccaggaaccg ctgcttttcc caagcctgtc gataaaagaa aacatcctgt ttgggctggc
     3361 aaaaaaacag ctctccatgc agaaaatgaa gaacttgctg gcggcgctgg gctgccagtt
     3421 tgatctgcat agtctggcag gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct
     3481 ccgcgggctg atgcgcgact cgcggattct gatcctcgat gaacctaccg cctcgcttac
     3541 ccctgcggaa accgaacgct tgtttagtcg cttgcaagag ctgcttgcta ctggcgtggg
     3601 tattgttttt atctcgcata agctgccgga aattcgccag attgccgatc gaattagcgt
     3661 gatgcgcgac ggaaccatcg ctttaagcgg caaaaccagc gaactgtcta ccgacgacat
     3721 tattcaggcc atcacgccag tggtacggga aaaatcgctc tctgccagcc aaaaattatg
     3781 gctggagtta cctggtaacc gcccacaaca tgccgcagga acgtcggtgc tgacactgga
     3841 aaatctgact ggcgaaggtt tcaggaatgt cagcctgacg ctaaatgccg gagaaattct
     3901 gggtctggct gggctggtgg gtgccggacg cacagaactg gccgagacgc tctatggtct
     3961 acgtactttg cgtggcggac gcattatgct gaatgataaa gagatcaata gattctccac
     4021 tggagaacgt ttactgcgcg gtctcgttta tctgccggaa gatcgccagt catccggact
     4081 gaatctcgat gcttcgctgg cctggaacgt ctgcgccctt actcataacc ttcgtggatt
     4141 ctgggcgaaa accgcgaaag ataatgccac cctggaacgt tatcgtcggg cgctgaatat
     4201 taaatttaac caaccggaac aagctgcacg gacattatcc ggtggcaacc agcaaaaaat
     4261 cctcattgcc aaatgcctgg aagcttcgcc gcaagtattg attgtcgatg agccgacgcg
     4321 cggcgtggat gtctcggcgc gtaatgatat ctaccaactg ttgcgcagca tcgccgcaca
     4381 gaatgtggct gtgctgctta tctcctctga tctggaagag atcgaactga tggcagatcg
     4441 tgtgtatgtg atgcatcagg gcgaaattac ccactctgca ctgaccgggc gcgatattaa
     4501 tgtcgagact attatgcgcg ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg
     4561 aagtttattc agaacaaccg tgaaatcacg gcactgctgg cggtggtgct gctgtttgta
     4621 ttacccggtt ttctcgaccg ccagtattta agtgtgcaaa cgctgaccat ggtttatagc
     4681 agcgcgcaaa tcctgatcct gctggcaatg ggcgcgacgc tggtaatgct tacgcgcaat
     4741 attgatgttt cagtgggttc gattaccgga atgtgcgcgg tgctgttggg gatgttactg
     4801 aacgcaggat attcactacc tgttgcctgt gtcgcgactt tactgcttgg tttgctcgcg
     4861 ggatttttca acggtgtcct ggtcgcgtgg ctaaagatcc ctgccattgt tgccaccctt
     4921 ggcacgttag ggttgtacag aggcatcatg ttgctgtgga ctggcggtaa atggattgaa
     4981 gggttacccg ccgaactgaa acaactctcc gccccgctac tgtttggcgt ttccgcgatt
     5041 ggttggttga cgataattct ggtggcattt attgcctggc tgctggcaaa gacggcgttt
     5101 ggacgcagtt tttatgccac aggcgataat ttacagggcg ctcgtcaact gggcgttcgt
     5161 acggaagcca ttcgcattgt ggcattttcg ttgaacggct gcatggcggc actggcgggg
     5221 attgtgtttg cttcgcagat tggttttatc cccaaccaga ccggtaccgg gctggagatg
     5281 aaagcaattg cagcctgcgt gctgggcggc attagtttgc tcggcggttc cggtgcgatc
     5341 atcggtgcgg tactcggcgc atggttcctg acgcagatcg atagcgcact ggtgctgttg
     5401 cgcattccgg catggtggaa cgattttatc gcgggcctcg tgctgctggc ggtgctggtg
     5461 tttgatggac gcctgcgttg tgcgctggaa cgtaatctac ggcggcaaaa atatgcccgc
     5521 tttatgacgc caccgccatc cgttaaaccc gcttcgtcag gtaaaaaacg ggaggctgca
     5581 taatgcgtat tcgctacggt tgggaactgg ctcttgccgc actgctcgta attgagattg
     5641 tcgcatttgg tgcaattaac ccgcggatgt tagacctcaa tatgttgctg ttcagcacca
     5701 gtgattttat ctgcattggc attgtcgccc taccgttgac gatggtgatt gtcagtggcg
     5761 ggatcgatat ttcgtttggt tcgaccatcg gcctctgcgc cattgcattg ggcgtgctgt
     5821 ttcaaagtgg tgtgccgatg ccgctggcga ttcttctgac cttactgctc ggcgcatttt
     5881 gcgggctgat caacgccgga ttaattatct ataccaaagt taacccgctg gtgattacgc
     5941 ttggcacgct gtatctgttt gccggaagcg ccctgctgct ttccgggatg gccggagcga
     6001 cggggtacga aggtattggt ggattcccga tggcgtttac agatttcgct aacctggatg
     6061 tgttggggct ccccgttccg ctgattatct tcctgatatg tctcctcgtt ttctggctct
     6121 ggctgcataa aacccatgcc ggacgtaatg tgtttttgat tgggcaaagc ccgcgcgtgg
     6181 cgctttatag cgcgattcca gttaaccgca ccttatgtgc gctctatgcc atgacggggc
     6241 tggcgtctgc ggtcgccgct gtgctgctgg tatcgtattt tggttcagca cgttccgatc
     6301 tcggtgcgtc gtttctgatg cccgccatca ccgccgtggt gcttggcggt gccaatattt
     6361 atggtggttc cggttccatt atcggcaccg ccattgcggt tttattagtg ggatatttgc
     6421 aacaaggttt gcaaatggca ggagtgccaa atcaggtgtc cagcgccctt tccggtgcgc
     6481 tacttatcgt cgttgtcgta ggtcgttccg ttagcctgca tcgccagcaa attaaagagt
     6541 ggctggcgcg tcgggccaat aacccattgc cataaaggat atcttcatga cacttcatcg
     6601 ctttaagaaa atcgccttac ttagcgctct tggcattgcc gcaatctcta tgaatgtgca
     6661 ggccgcagag cgtattgcat ttattcccaa actggttggc gtgggatttt ttaccagcgg
     6721 tggcaacggc gcacaacaag cgggtaaaga gctgggcgtt gatgtgacct acgacgggcc
     6781 gacagaaccc agtgtttctg gtcaggtaca gttgattaat aacttcgtca atcaaggtta
     6841 taacgccatt atcgtttctg cggtttcgcc tgatggcttg tgtccggcac tgaaacgcgc
     6901 catgcaacgt ggtgtgagag tgctgacctg ggactctgat actaaaccgg agtgccgctc
     6961 ttactacatt aatcagggaa ctcccgccca gttgggaggt atgttggtgg atatggcggc
     7021 gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc tactcaagcc ccaccgttac
     7081 ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc gccaaagagc atcctggctg
     7141 ggaaattgtc actacgcagt ttggctataa cgatgccact aaatcgttac aaaccgcaga
     7201 aggaatatta aaagcgtata gcgatctcga cgccattatc gcccctgatg ccaacgccct
     7261 gcccgctgcc gcacaagccg cagaaaacct gaaaaatgac aaagttgcga ttgtcggatt
     7321 cagtacgcca aacgtgatgc gtccatatgt ggaacgcggc acggtgaaag aatttggcct
     7381 gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc gcggatgcat tattgaaaaa
     7441 aggatcaatg aaaacgggcg acaagctgga tatccagggc gtaggtcagg tggaagtctc
     7501 gccaaacagc gttcagggct atgactacga agcggatggt agtggcatcg tactgttacc
     7561 ggagcgcgtg atattcaaca aagagaatat cggcaaatac gatttctgat gtgcattact
     7621 taaccggagt aagttatggc agatttagac gatattaaag atggtaaaga ttttcgtacc
     7681 gatcaaccgc aacaaaatat cccttttacc ctgaaaggtt gcggtgcgct ggattgggga
     7741 atgcagtcac gcttatcgcg gatatttaat ccgaaaacgg gtaaaaccgt gatgctggcc
     7801 tttgaccatg gttattttca gggaccgact accggacttg aacgcattga tataaatatc
     7861 gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt gcgcagcgta
     7921 gttccccctg cgaccaatag gccggtggta ctgcgggcgt caggtgcgaa ctctattctg
     7981 gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg acgccgtgcg cctgaacagt
     8041 tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc gatcaaaaat
     8101 attattcagc tggttgatgc cggaatgaaa gtgggaatgc cgaccatggc cgtgactggc
     8161 gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg aatcgccgct
     8221 gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga acggattgtt
     8281 gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga gcgcgaggcg
     8341 ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat ggggcgtaat
     8401 attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt ggtacaccat
     8461 aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta actgcggatt
     8521 aaggagaaga aacatgcacg tcacactggt tgaaattaac gttcatgaag acaaggttga
     8581 cgagtttatc gaagtttttc gccagaacca cctgggctct gtacaggaag aaggcaattt
     8641 gcgcttcgat gtcttacagg acccggaagt gaattcgcgc ttttatatct acgaagccta
     8701 taaagatgaa gacgcagtgg cgttccataa aaccacgccc cactacaaaa cctgtgtcgc
     8761 gaaactggaa tctttaatga ccgggccgcg taaaaaacgt ctgttcaatg ggttgatgcc
     8821 gtgaggcgaa tttatccggt gcgccgtaaa accccgtcct tcagggcggg gatataaggc
     8881 gcggttttcc acctgaccag gtgtttgctg ttgttcaatg tattggcgga tgacggatat
     8941 tggcgcacct ccgcagctac tggcaaaata gccaggactc cacaaaacgc ctttgtagta
     9001 atacctgact gcaatatctg gtcgatctcg tcgcagtaac ctacctgata cgtttttgag
     9061 gctgtttacc agactggata tcgccagttt gggaggatag ttaattaaca aatggacgtg
     9121 atctggttcg ccatccattt caaccagttc agcttcaaaa tcagcacata catttgaaaa
     9181 gtaagtgcgt agtttttctg tcgcgtcatg gtcaaaaatc tggcgtcggt atctggtgac
     9241 aaagaccatg tgaacatgct tcaggaaaac acaatgcctt ccccgtcgga tatcggtttc
     9301 tttattcata gaccaaagta tcatttggcc tgtgaaacga ctacaggcat ttaaattcca
     9361 gttaagaccc ggtggtcaac aggagcgtga aatgaggcgc ttcgcaggcg catgtcgttt
     9421 cgttttcaat cgtgctctgg cacgtcagaa tgagaatcat gaggccggta ataaatacat
     9481 cccttacggg aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg
     9541 gcttaaagat gccccgtcac agccattgca acagtcactg aaagaccttg agtgggctta
     9601 caaaaacttc ttccggaagc gggctgcttt tccccgattc aaaaagcggg gacagaatga
     9661 tgcattccgc tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc
     9721 gaaactgggc tggatgcgct accggaatag ccgtcaggtc acgggtgttg tgaaaaatgt
     9781 cacggtcagc cagtcctgcg gtaagtggca catcagtatt cagacagaaa gtgaagtatc
     9841 aactccggtt cacccttcag catcaatgat cgggctggat gctggcgtgg ctaaactcgc
     9901 cacgctgtca gatggcacag tctttgaccc tgtaaacagt tttcagaaaa accagaagaa
     9961 gctggcgaga cttcagcgac agttcagccg caaggtcaaa ttcagcaaca actggcagaa
    10021 gcagaaacgc aaaatacagc gactgcattc ccgtatcgca aatatccgca gggactacct
    10081 tcacaaagtc acaacgaccg tcagcaaaaa ccacgcaatg atagtcattg aggatttgaa
    10141 ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc cagccggggc gcaatgtccg
    10201 ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgtcgcca
    10261 gcttgagtac aagcagctct ggtgtggcgg tcaggtgctt gctgttccgc cagcgtacac
    10321 aagccagcgt tgcgcgtgct gtggtcatac agcgaaagaa aatcgcgtgt cacaaagtaa
    10381 attcagatgc caggtatgtg gatatacagc gaacgccgat gtaaacggcg ctcgtaacat
    10441 tttagcggcg gggcacgccg ttcttgcctg tggagggatg gtgcagtcag gccgcccgtt
    10501 gaagcaggaa cccaccgaaa tgattcaggc gacagcctga acgtagcagg atcctcgtca
    10561 ttcagggcgt ggaggatgtc aacagtttta tctacaattg gggtaacgcg ctgacgggag
    10621 taaaaaaatg tctgactgga acccctcttt atatctgcac tttgccgctg aacgatcgcg
    10681 tccggcggtg gagctgcttg ccagagtgcc gctggagaat gtcgaatatg ttgccgatct
    10741 tggctgtggc ccaggtaaca gcaccgccct gctgcatcaa cgttggcctg gtgccaggat
    10801 aacgggcatc gactcgtctc cggcgatgat tgctgaagcg cgcagtgctt tgccagactg
    10861 ccagttcgtg gaagcagaca tccgcaactg gcaaccggaa caggcactcg atctgatttt
    10921 tgctaatgcc tcactgcaat ggctgcccga ccactacgaa ttgtttcctc acttggtttc
    10981 tttacttaat ccgcagggcg tgctggcagt acagatgcca gataactggc tggagccgac
    11041 ccatgtgctc atgcgcgaag ttgcctggga acaaaactac ccggatcgcg ggcgtgagcc
    11101 gttggctggc gttcatgctt actacgatat tttgagcgaa gccggatgtg aggtcgatat
    11161 ctggcgaacc acctactatc accagatgcc gtcgcaccag gcgattatcg attgggtgac
    11221 cgccactgga ttacgtccgt ggttacagga tctgaccgag agcgaacagc aactttttct
    11281 tacgcgctac catcagatgc tggaagagca gtatccactg caagagaacg gacagatact
    11341 gctggcattt ccgcgtctgt ttattgttgc ccgccgtacg gagtaaatat cacgtcagtt
    11401 ggtaatggcg atcgggaaga atttttgctg gaatttcggc ttcatcgttc atctgtaaca
    11461 ggtcaatttc aatagcgtta cagatggcat ccagtggtaa atcattgttt tcaataccga
    11521 acggatcttc cagttcttcc gccagacaat ccagcgaaat aaaagtgtag gaaatcagca
    11581 cagaaataaa aggcgtcatg taatgcaggt ccacgaccag cgcgaacggc agcatgatac
    11641 aaaacagata aacggtacga tgcaaaatca gggtataggc aaagggaatt ggcgtgtagg
    11701 caatgcgctc gcagcctgcc agaaccgctg aaatatcgtt aagccgatcg ttgaggctaa
    11761 taaacaggat atctgaaagc tgtccattgc ggcgctgaac cgccaaccat tctcccatta
    11821 ttaacaagat acggttagct ggagagttcg aagccagtac acgctgaaga tcttcagtct
    11881 tgagataatg agccagcact tccgcctgtg gctgtttgcg taatgtcatg cgtaaacagt
    11941 gggcgaaggc gatttgcagc cgggcaaact cccttacact tgccgaatcc ggcaatgtcg
    12001 tttttacctc gcgcagtaac gaccgtgagg caatcatcaa ctgcccccaa agttttcgcg
    12061 cttcaacgta acgggcgtaa ccggcattat tacgaaaacc aagaaaaatg gcgatggcga
    12121 caccgagaat gctgaacggt gcgagggtga atttgatgcc cagatgcgtg taccagggca
    12181 gcatgaaaat aacagcgata gaaaagagaa aattgagtag taagcgcgag gatatcttgg
    12241 ataatactga gccatgccag acaaaaatac ggcgcagcca gtgttgttgt ggacgaacga
    12301 tcatggttat cttcaggcgt ggaaaagtcg ccctattaaa cgtgattacg atcacattct
    12361 caagacacga cttacaaatt atctacaagc ttacaacatt aacaggtgct gcatgtggca
    12421 cctgttaatg ataaaggcat ataggatgtt gtaactaata tagtgaaatg agaaacccgg
    12481 tcgaaaccgg gttcagagta ttagcacaac ggacgtacag cttcgcgcat ccctttttca
    12541 agaatcgcat ccaggtcatt agcaacctgc tcgaccagac caggcacttg cgtcaggtcc
    12601 tgctcccagt ggtctttttc tgccaacaca atcgctacca gttcctgggt accgataaca
    12661 cggtcacgat gctggctcca gaggtgctga taacgttcca gccagtgtgc atcgtcctgt
    12721 accggatatg tttctccgtt acgctcaccg cgatagaacg caatcaatgc cgctaatgcg
    12781 aaagtaaggc gcgccggaag tgtgccgttt gccttctgcc ctgccagtag ctgcggcagg
    12841 atgcgggtac ggaacttggt cataccgttg agcgcgatag acagcagctg atgcttgatg
    12901 tacgggttac ggaaacgccc ggtgactgca ctggcgaaag actccagttc atcgcgaggc
    12961 aaatccagaa ccggaataat ttcttcgtag atggctttct caacgaatgc gcaaatttca
    13021 gcatcgttca tcgcttcacc tacggtatcc agccctgcct ggaacgccac cggcaccagc
    13081 gcggtgtgtg caccgttgag tattgccact ttgcgttctt tatacggttt aatatcgtca
    13141 acaatcagca cgttgagcgg atatttgtcc agacgcagtt cagtcgctaa ggatttcggc
    13201 ccctgaatca caaacaggta aaagtgttca gcggtgtcga ggaaaccatc gtgataaccc
    13261 agttcttctt ccagtttagc cacttcatca cgcggatagc cggtaacgat acggtccacc
    13321 agcgtagaac agaagctgtt agcctgatcc agccattgaa taaatgcttc tggcagtgcc
    13381 cactcttgcg catagcgcag caccagttca cgcaacgcgt cgccattgta gtcgatcaac
    13441 tcacacggga tgatgatcca acctttatcc agcgcaccgt tgaaatggct aaaacgttcg
    13501 aacagcagac gggtcagttt tgccggataa cttaccgctg gcgcgtcatc gaatttatcg
    13561 cccgcatggt agctgatacc cgcttcggtg gtgttagaga aaacaaagcg catttccggg
    13621 ttgtgcgcca gtttcaggaa ttcatcgtat tcactgtaga cgctgatttc acgattaaca
    13681 gagcgaatca gacgcgcgtc gctgaccgct tcccccttct cattcaggcc acggataatg
    13741 gtggtgtaca gaccatcctg cgtgctcagt gacggcggga atgaagtttc aatcggacga
    13801 acaacgacca caccagaatt cagatcggtg tgctcattca ggagatctat ttgccagtca
    13861 acaaaggcgc gcaggaagtt accttcacca aactgaatga tacgttctgg atactgtgca
    13921 ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt cccttctgat tagtcataca
    13981 acctgtttga attggtacga caggttagca aactttaata cgccgaaccc ctgttttgat
    14041 caactcctga tgattaatga gcagttttat gagaaaagtg tggcgcggat catggtttaa
    14101 tcgaggaaat acgccttttc ctggatcata aagtggtaga acacattgca ttcaaatcgc
    14161 tctgaatgaa taaagatgtc agacaacttc ctcaccgtaa cgcatagtgc tggtacggtt
    14221 gcgcccatct ttcttcgagc gatacagaca cgtatcggct tcgaccatca atttattaaa
    14281 gtcatcggtc agcgttcggt acgatgcgcg accactaccg acgccaatac ttaccgtgag
    14341 ataaagcgtt ttttgttgcc aggtgaatgg ttgcagttca acgcctttac ggattttttc
    14401 cgccattagc agaccatcga caggattcac cgacggcact gcaacagcaa attcttcgcc
    14461 gcccattcgc gccaccagcc ccttgtcacc gacaatcttc tggatatgcc gggcaaacac
    14521 gcttaacact ttatcgccac attcatgtcc atagttatcg ttaatacttt tgaaatagtc
    14581 gatatcaagc agcatgacag tcagatgttg tgtctgtttc agcgatggac ttttcagcgc
    14641 ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa tcaaagtcgg ctcgcagcgc
    14701 aacttgcttc attagcgaat tgatcgctgc cacgctaaaa gaaaccataa ttgggcatat
    14761 cgccatcgtg gcaataccga gacgtgcgga gaacatttcc ggaatggaga acggcgaacc
    14821 gaccgagata tcaatcaccg aattagccac cagtacgatt tccaccgcac cggtgacaaa
    14881 ggtcaacaga catgttacct gcggcgtata gcgcactgca caccagatta atgcaggcag
    14941 cggaaacgcc agactccccg ctccgccaat gactaccgag gcaataaccg acacaataag
    15001 cgcgatcgcg ggcatcatct gttctgcttt aaagcgcggc agcactccag gaatagccaa
    15061 cgtcagcata caaggtacga tcaacacgcc cgttgagaat tgctcactga accagtccgc
    15121 aagcaaaggc cagaaatcca gactatcaat actgaccgaa ccaatcgccc cgacaatagc
    15181 gcataataat gcggcaatca gacagtaatt aaatagccgt aaggcactta ctggctcata
    15241 tttatttttc cccaaacgct tatcccgcac gaccagtaag gcgacggtaa caataaacat
    15301 catattggag aaattgatag ccagtgaaac cagtccccat tcggtggtta tggcatcata
    15361 aaccagcatc gccacataac tgatcgcata gtaatgcagt cgattaagcc agacatagcg
    15421 ggcaaacacc cccgccatta cgccattcag cggccagaaa agagaaagtt cgtgaaccag
    15481 ccgtaactct gcaccaataa aatagaacag cgtggttagc gcaaagatag cgatgctatt
    15541 acgtagcaga tgaccttcct ggaacacacg gaaagttgaa atggggtgta catgcattaa
    15601 ttatctgtcc atacaattgc gggtggcaat tatctcaacg taataagtaa attctcataa
    15661 aggatagcat gttcgctgac agcggcggca aataaaggta ccctgggaat gctattttgt
    15721 cgttttccgc aattctcatg gctgattcat ctgtacaacc agccagtgat agattaccgc
    15781 cacgatataa cgttgttgtt cttcgcttag cgaaacaccc tgcggaatgt tatgccactt
    15841 agccagacaa ccgcgacagc aggtcgcggt ggcgtgctga gcgataaaca ccggatgccc
    15901 gcgcatgggg gtttgcttac cgtcattagc cggtaatgcc ggagcaagac gtttagcgac
    15961 aaaatccgcc gcatgttgtt cgatgactgg cgcgcctttc tccagacaat actgacgctc
    16021 tttcatgtcc aaacgaaagc gagatcgaaa ttttgaacgg gataaacgcg cgaagagtgt
    16081 atcgagagac tgcattaata aaccgaacgc cctaactgtt tcgtcaattg ttcaaggacg
    16141 gcgatacccg caagcgagtt acctgcatca tccagttccg gactccagac tgcgatggcc
    16201 atttcatgcg gaacaatcgc cacaataccg ccaccaacgc cagatttcgc cggtagtccc
    16261 acccgccagg caaactcccc cgcgttctgg tacataccac tggtcgccat cagcgcgtta
    16321 atttgccgtg cctgcattgg cgtcaccaca ggttcatcaa tatgaatagc tttcccctga
    16381 ttagccagaa agacaaacgt ccgggccagc tctacacagc tcattttcag agcgcagtaa
    16441 tgaaaatagt tttgcagaac ggttgtcacg tcatgatgga aattgccaaa cgacttcatc
    16501 agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt cggaacgcgc taccaccgta
    16561 tcgtaggaaa tatcagacac accgcttaag ccgcgcacga cttccagcat acgttgccgt
    16621 ggtgcgctta atcgcccttg caacatatcg cagaccacca gcgcaccagc attaataaac
    16681 ggattacgcg gaataccctg ctccatttcc agttgcacta aagaattgaa cggtgatcca
    16741 gacggatctt tgccgacacg ttgccagatt tcctcttcgg agtaatgacg catggcgaca
    16801 acgagactca gcactttgga aatagactga atggaaaaac gttcttgcgc gtctccggcc
    16861 tgaaaaagct gtccgtcaac ggtacagata gcaatgccca atcgggaacc gtctactgta
    16921 gccagcgccg gaatataatc cgcgacttta ccctgaccaa tgagcggccg cacttgccgc
    16981 aagatgtttt ctaaaattgc attatccatg gcgactgcca ctttctactc ctggaccgca
    17041 ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt cgcgtcagat ccggtctttc
    17101 cacaccgtct ggatattaca gaattcgtgt aagccgaaat gggaaagctc acgaccaaag
    17161 ccactctttt tcacgccacc aaaggccact cgcgcatcgc tggcacaata accattgata
    17221 aacaccccac cgcattccag acgtgccgcc atctgtctgg cctgtgtttc gtcggtggta
    17281 aaaatggtcg ctgaaaggcc gaactcacta tcattagcca gttccagcgc atgttctgca
    17341 tctttcgcaa cggtgattgc cgcaacgggg ccaaacattt cttcccgaaa cgcggtcatt
    17401 tctggggtaa cattcgccag aaccgttggc ggatagtagt tacctgcccc agccatcttt
    17461 tccccgccca gtaacaaacg cgcaccctgc gccagggttt tctccacctg atgatgcagc
    17521 tcatcacgta aatcgaaacg agccattggt ccgagagcgt tctcttcgtc acggggatcg
    17581 cccattttca aggctgccgc agctgccaca aaacgttcgg taaatgccga agcaattccc
    17641 tcttcgataa taaagcgttt tgccgctgca caaacctgtc cggtattctg ataacgtccg
    17701 gctaccgccg ctttaaccgc cagttccaga tcggcatcgt taagcacaat aaatggatcc
    17761 gaaccgccca gttccagtac gcattttttc agtgccgctc cagcctgtgc accaatagcc
    17821 gctcccgcac gaacacttcc ggtcaccgtg acagcagcaa tgcgcgagtc tttaatcatc
    17881 tgactgacac cgtcgttgtg ggcattcagc cagccatata cgccttgtgg gattcccgca
    17941 tctttaaaca cctgggcaat gagctgtgca cagcccatca cattcggcgc atgtttaagt
    18001 aagtagccgt tacctgcaag aatgatggga acagcgccac gcatcacctg ccataacgga
    18061 aaattccacg gcataatcgc cagaatcgtc cccaacggtc gatactcaat aactgcctgc
    18121 tgattttcca ccagcgtagg ttccgccttc agcattgccg gaccatgttc tgcataccag
    18181 tcacacaaat tcgccgattt cgccacttca gcgcgcgcct ggttgattgg tttgcccatt
    18241 tcgcgggtga tcatttgcgc catttcttcg ctacgggcgc gcagagcctt accgatatca
    18301 cgcagttttt cagcacgata atctatattt gtctcgcgcc agtcacgaaa gcctgctgcc
    18361 gccagctgaa gtgcgttttc gatatcatca gcgccagccc acggcagcac agaaagttgt
    18421 tcacccgtgg caggatttat cgaaattgca tgagttgccg gagtaatggt catcggggta
    18481 tctcctttat gagtcatggt atgaagatac gcagatttac tcttgcttta aaatgaataa
    18541 tattaagcca cttattcacg aatcgagaat attatggatc tgacccaact ggagatgttc
    18601 aacgcggttg ccgaagctgg cagcattacg caggctgcag caatagtgca tcgcgtgccg
    18661 tcgaatctca ctacccgtct acgccagctg gaaacagaac tgggggtaga tctgtttatt
    18721 cgcgagaatc agcgtttacg tctatctcct gccgggcata actttttacg ctatagccaa
    18781 caaattctcg cgttagtgga tgaagcgcgg agcgttgtcg ctggcgatga gccgcaaggt
    18841 ttattttctc tgggttcgct ggaaagcacc gcagcagttc gcattccggc cacgctggcg
    18901 gaattcaacc gtcgttatcc caaaattcag ttttcacttt ccaccggccc ttccggcact
    18961 atgctggaag gtgtgctgga gggaaaactt aatgcggcgt ttattgatgg acccattaac
    19021 catactgcca ttgacgggat accggtatac cgcgaggagc tgatgatcgt cacgccacaa
    19081 ggtcatgcgc caataatccg tgccagtcag gttaatggca gtaacattta tgccttccgc
    19141 gccaattgtt cgtatcgtcg ccacttcgag agctggtttc atgctgacgg tgccgctccg
    19201 ggaactatcc atgagatgga gtcttatcac ggaatgttgg cctgtgtggt cgcaggagca
    19261 ggtattgcgc ttattccgcg ctctatgctg gaaagtatgc cggggcatca ccaggttgaa
    19321 gcgtggccgt tagctgagca atggcgttgg ctaacaacct ggctggtctg gcgtcgtggt
    19381 gcgaaaacgc gtccgctcga ggcatttatt caactactgg atgtgcctga ctcgacaaaa
    19441 cagggttacc aatgagctat ttttgatagt tcttgcggct aatatgctct atatagtgat
    19501 gttccgatga cttatgacta tatggggcaa atatggttac gccagtaagc atcagcaatt
    19561 acatatctct tcctgatgat tttcccgttc gtaatattgc accgcaggta aaagaagttt
    19621 taaaggattt tattgatgca cttagtacaa taatatgtaa tgaagagtgg cgcacgtctt
    19681 taaacatcaa ctcagccaca aaaaagatat ttaataacct tgacaactta tcatatatt
//
LOCUS       DAPGRN010000091        19722 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPGRN010000091 DAPGRN010000000
VERSION     DAPGRN010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.091"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..1004
                     /gene="robA"
                     /locus_tag="RHW33_003374"
     CDS             135..1004
                     /gene="robA"
                     /locus_tag="RHW33_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV1646848.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(1001..1648)
                     /gene="gpmB"
                     /locus_tag="RHW33_003375"
     CDS             complement(1001..1648)
                     /gene="gpmB"
                     /locus_tag="RHW33_003375"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV1646849.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGALEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            1700..2212
                     /gene="yjjX"
                     /locus_tag="RHW33_003376"
     CDS             1700..2212
                     /gene="yjjX"
                     /locus_tag="RHW33_003376"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV1646850.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(2359..2685)
                     /gene="trpR"
                     /locus_tag="RHW33_003377"
     CDS             complement(2359..2685)
                     /gene="trpR"
                     /locus_tag="RHW33_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV1646851.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(2775..4712)
                     /gene="sltY"
                     /locus_tag="RHW33_003378"
     CDS             complement(2775..4712)
                     /gene="sltY"
                     /locus_tag="RHW33_003378"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV1646852.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTKGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            4923..6590
                     /gene="ettA"
                     /locus_tag="RHW33_003379"
     CDS             4923..6590
                     /gene="ettA"
                     /locus_tag="RHW33_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV1646853.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(6888..8120)
                     /gene="nadR"
                     /locus_tag="RHW33_003380"
     CDS             complement(6888..8120)
                     /gene="nadR"
                     /locus_tag="RHW33_003380"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV1646854.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(8141..9523)
                     /gene="radA"
                     /locus_tag="RHW33_003381"
     CDS             complement(8141..9523)
                     /gene="radA"
                     /locus_tag="RHW33_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV1646855.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(9572..10540)
                     /gene="serB"
                     /locus_tag="RHW33_003382"
     CDS             complement(9572..10540)
                     /gene="serB"
                     /locus_tag="RHW33_003382"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV1646856.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            10646..11290
                     /locus_tag="RHW33_003383"
     CDS             10646..11290
                     /locus_tag="RHW33_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV1646857.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            11318..12334
                     /gene="lplA"
                     /locus_tag="RHW33_003384"
     CDS             11318..12334
                     /gene="lplA"
                     /locus_tag="RHW33_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV1646858.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            12366..12629
                     /locus_tag="RHW33_003385"
     CDS             12366..12629
                     /locus_tag="RHW33_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1646859.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(12790..13509)
                     /gene="deoD"
                     /locus_tag="RHW33_003386"
     CDS             complement(12790..13509)
                     /gene="deoD"
                     /locus_tag="RHW33_003386"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV1646860.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(13566..14789)
                     /gene="deoB"
                     /locus_tag="RHW33_003387"
     CDS             complement(13566..14789)
                     /gene="deoB"
                     /locus_tag="RHW33_003387"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV1646861.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(14841..16163)
                     /gene="deoA"
                     /locus_tag="RHW33_003388"
     CDS             complement(14841..16163)
                     /gene="deoA"
                     /locus_tag="RHW33_003388"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV1646862.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(16290..17069)
                     /gene="deoC"
                     /locus_tag="RHW33_003389"
     CDS             complement(16290..17069)
                     /gene="deoC"
                     /locus_tag="RHW33_003389"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV1646863.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            17327..18877
                     /locus_tag="RHW33_003390"
     CDS             17327..18877
                     /locus_tag="RHW33_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV1646864.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDVFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEIGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            18849..19712
                     /locus_tag="RHW33_003391"
     CDS             18849..19712
                     /locus_tag="RHW33_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV1646865.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALRERG
                     VSRLIFPALYL"
BASE COUNT         4550 a         5463 c         5144 g         4565 t
ORIGIN      
        1 caatactttt atttccgtca agacctaaaa catactctac taagaaaaaa acactgaatg
       61 ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg ttgttgaaag
      121 gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc tggctggaag
      181 gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt tattccaagt
      241 ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg tatattcgtg
      301 ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg attctggaca
      361 tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc aagaagcagt
      421 ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt ggtattcgcc
      481 cgccgctgcg tctgggtgaa ttcactatgc cagagcataa atttgtcacc ctggaagata
      541 cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc tctgatttcc
      601 gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg accattccgc
      661 cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac gaacaagagg
      721 tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg acggggcatc
      781 cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg ggaaccggcg
      841 tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac ctgacgcgcc
      901 gtaaaggtca ggatattgag cgatactacc cggcagaaga tgccaaagcg ggagatcgcc
      961 caattaatct gcgctgtgaa ctgctgattc cgatccgtcg ttaacgctgc agctcatcta
     1021 atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag ccagacgcca
     1081 gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga cgtaagcggc
     1141 gctctgccca tgctggtaat ccgagaatcg tactcaccag gcatcccagt gcaataccat
     1201 gactcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc agtgccgcat
     1261 tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg tcaacggtgc
     1321 cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg atatgtcttt
     1381 tttccagcgc acccatgttt aattcacgca ggcgagaatc aaagatgatg tcacagccgc
     1441 aggcctgggc gatgatttcc gccgtacgcc gggtgcgccc taaatcgcta ctgatgatat
     1501 gcgtaatgcc aagctctttg gcacgcgtcg ccacctgcat cgcctgttgc tcacctttgg
     1561 cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac tgcgtttcac
     1621 cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg taacgttaat
     1681 tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc ccgctaaaat
     1741 tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc atattgcatc
     1801 cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa cgcgcgctgg
     1861 cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt tttgggtggc
     1921 gattgaagct ggcattgatg gcgacagcac tttcagttgg gtggtgattg aaaacgccag
     1981 ccagcgcggc gaggcgcgtt cggctacttt gccgttgcct gcggtgattc tggaaaaagt
     2041 gcgtgaaggt gaagcgcttg gtcccgtgat gtcgcgttat accggtattg atgagattgg
     2101 tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg ccagcgtcta
     2161 tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat gcggtgtatt aagcgcctgg
     2221 tgcgacgctg ccgcgtctta tcatgcctac caaacatatt gaaattacgg gtatttgtag
     2281 gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga tgcgccacgt
     2341 cttatcaggc ctacaaaatc aatcgctttt cagcaacacc tcttccagcc actggcgcag
     2401 ctcaacgggc gcggctttca ggctgttaga tccacgcgta atcgtcgcga tgcccgcgcc
     2461 gagttcattt tttaactcac gctggctcat ttcgccgcgc aacagctctt cgacaatacg
     2521 cacgcgagtc cccaacgctt cgcgctcatc tggcgtcagc atcaggttta acaacggtaa
     2581 atggagatcg ttttggtagg cattcttaag caggtcgaca aaacgtaacc actcctggtg
     2641 acgctgttct gccatcgctg ctgaataggg tgattgttgg gccataatat gtcgccattg
     2701 ttagcggggg aagcaaaatg cctcccccgg ttgtactcgc taaagagtac gatagcatat
     2761 cataaacgtg tggatcagta acgacgtccc cattccgtgg cgctcatcaa cgtcggttta
     2821 tcccccatga aatagcggta gtaagcgtca taagccagca cgttcttcac ataaccgcgc
     2881 gtctcggaga acggaatact ctcgacaaat gccacagcgt cgatacgccc ggcgctgttg
     2941 ccaagccagg ttcgcacccg ccctggtccg gcgttataag ctgctgagga gaaaatacgg
     3001 ttattaccaa attgctgata aacatattgc aggtaactgg tgccaatgtt gatattcgtt
     3061 tccggatcca gcaattgccc agggctgcta taaccaggaa tagagaacat cttcaccgta
     3121 tgggtcgccg tgcccggcat aatctgcatc aggccgctgg cccctaccgg tgatttcact
     3181 ttcggattcc aggcgctctc ctgacgggca atcgccatcg catagctttg cgggatctcc
     3241 ttcccactgg tgtaacgttt gaaaagatcg ttgtaagcca gcgggaatcg ctcttccaga
     3301 tgatcccaaa gcttcccggc gatcgttgcc tgaacgctaa gatcccacca ttggttgttg
     3361 aaagcatacc gcgccagttg agcttgctct gtttttgact tgctcttcac cagattggcc
     3421 cactcgctac gcgcggtgtt atcgagattc cagtacatca actcgcgcac gcgcgccatc
     3481 tccggcccct tagtcagggc gctgtcaaca ttctgcggcg ctttatcaat cttcagctca
     3541 tactcttctc cgatgcgttg cgctgcaacc atcgggtaga aaccacgctg ttgcatgagt
     3601 tgatgcaaaa tctcttttgc ttcagcttca cgtccgcgtt ccagcaataa atccgcctgc
     3661 cagtaacgcc attcatcttt ctctttcgcc tccatcggca aacgcgccag ccaggtattc
     3721 aggccgcgac gatcgccagt gccaagcgcc attcgcacac ggcgttcaat aagcgaagta
     3781 gattgcgagc gcataatggc gtcatcgcgc catttcgcct gctcgtcggt gacatcgttg
     3841 cccatcaaac gccaggcgac gatatcgcgc agctcctgaa tctgatcttc attaagctgc
     3901 tgcgcctggg caagcgatgg gatcatcagc cgtgcatttt cagcatcctg ccgcgccaca
     3961 ctggcaaacg ccaccgccgc catttgacgg gtaaaatcgg tcgctccggt tgtacgcgcg
     4021 aaggtcagta ccgtattagg gttgttcgcc agtgaaatga ttgccgaggc gatagtctgg
     4081 taatcggcag gcatctgccc tgccagcact gttaccaggc ctgtgttacc cgctttcatc
     4141 gccagacgga tacgctctaa atacgccagc ggatcttgtt tacctgacgc acgccagacg
     4201 ctaaataact tgtcacaggc gttaggctgg ctcttgccgg ttagccacag ctctttcgcc
     4261 ccttgccagg cttcttcact ctgcccggtg ttccatttcg catagtagta attacattgc
     4321 gcttcggtag ttccgggctt ttccgggcta aaggctaaca agccacgcca gtcttcacgc
     4381 cgcgccagtt cattgacgaa acgagattgc agcgtgcgag cgggaggaag cgtggggtta
     4441 gcgcgaacaa agttagtgac cgtcaccgcc ggttgattca tcagatcatc ggtgatctgg
     4501 cggtattcca ggtagggata aagcggataa tccttcagtc caggcatcat ttgttcgacc
     4561 acatccattt gtcgattatc ccaggcctgc ttgatttgcg cgtaacgact acgctgctca
     4621 tccagtgagt cggctcgcgc cacgctgctg accgtcagca gacagacacc ggcagccaac
     4681 agccgccagg taacttgttt ggctttttcc acaagcgcat cctctaagtg taaatacatc
     4741 aatgcagcgt catgccgcgt aatagattta tgctaaccag tcattgccgt ttacgccacg
     4801 ttacggacac ttttttactt ttactgcgaa ggtgatcgac caactgggat taggcgggga
     4861 aaaaggctac acttcgcctt tgaaaactca tcatcacgat aaacatagag gcgaagtcca
     4921 acgtggctca attcgtttat accatgcatc gtgtcggcaa agttgttccg ccgaaacgtc
     4981 atattttgaa aaacatctct ctgagtttct tccctggggc aaaaattggt gtcctgggtc
     5041 tgaatggcgc gggtaagtcc accctgctgc gcattatggc gggcattgat aaagacatcg
     5101 aaggtgaagc gcgtccgcag ccagacatca agattggtta tctgccgcag gaaccgcagc
     5161 tgaacccgga acacaccgtg cgtgagtcca ttgaagaagc ggtttcagaa gtggttaacg
     5221 ccctgaaacg cctggatgaa gtgtatgcgc tgtacgccga tccggatgcc gattttgaca
     5281 agctggccgc tgaacaaggc cgtctggaag agatcattca ggctcacgac ggtcataatc
     5341 tgaacgtaca gctggagcgt gcggcggatg cgctacgtct gccggactgg gacgcgaaaa
     5401 tcgctaacct ctccggtggt gagcgtcgtc gcgtggcgtt gtgccgcctg ctgctggaaa
     5461 aaccagacat gctgctgctc gacgaaccga ccaaccacct ggatgccgaa tccgtggcct
     5521 ggctggaacg cttcctgcac gacttcgaag gcaccgtggt ggcgattacc cacgaccgtt
     5581 acttcctcga taacgttgcg ggctggatcc tcgaacttga ccgcggtgaa ggtattccgt
     5641 gggaaggtaa ctactcctcc tggctggagc agaaagatca gcgtctggcg caggaagctt
     5701 cacaagaagc ggcgcgtcgt aagtcgattg agaaagagct ggaatgggta cgtcaaggta
     5761 ctaaaggccg tcagtcgaaa ggtaaagcac gtctggcgcg ctttgaagaa ctgaacagca
     5821 ccgaatatca gaaacgtaac gaaaccaacg aactgtttat tccacctgga ccgcgtctgg
     5881 gcgataaagt gctggaagtc agcaacctgc gtaaatccta tggcgatcgt ctgctgattg
     5941 atgacctgag cttctcgatc ccgaaaggag cgatcgtcgg gatcatcggt ccgaacggtg
     6001 cgggtaaatc gaccctgttc cgtatgatct caggtcagga acagccggac agcggcacca
     6061 tcactttggg tgaaacggtg aaactggcgt cggttgatca gttccgtgac tcaatggata
     6121 acagcaaaac cgtttgggaa gaagtttccg gcgggctgga tatcatgaag atcggcaaca
     6181 ccgagatgcc aagccgcgcc tacgttggcc gctttaactt taaaggggtt gatcagggta
     6241 aacgcgttgg tgaactctcc ggtggtgagc gcggtcgtct gcatctggcg aagctgctgc
     6301 aggttggcgg caacatgctg ctgctcgacg aaccaactaa cgacctggat atcgaaaccc
     6361 tgcgcgcgct ggaaaacgcc ctgctggagt tcccgggctg tgcgatggtt atctcgcacg
     6421 accgttggtt cctcgaccgt atcgccacgc acatcctgga ctaccaggat gaaggtaaag
     6481 ttgagttctt cgaaggtaac tttaccgaat acgaagagta caaaaaacgc acgctgggcg
     6541 cagacgcgct ggagccgaag cgtatcaagt acaagcgtat tgcgaagtaa tgcgtaaaat
     6601 gccgggtgcg gcgcgaacgc cttatccggc ctacaaaacc agcaatttca atacgttgta
     6661 aaactgtagg cctgataaga cgcggtaagc gtcgcatcag gcattgagca ccgaatgccg
     6721 gatgcggcgt gaacaccgtt tccggtctac aacagcctta gcatcctcaa tttcgtagcg
     6781 ggtcacccgt acaggttctt tctaatctcg acagtctctt ccagagaggt tttcaactca
     6841 tcaaaaaaac tcattttata cctcgccttt gagcagtttc gtcacggtta tccctgctcc
     6901 cccatcatct cccgcaccag ctccacgcag cgcaggaaac ggctgtcata atcgtcctct
     6961 tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa gttctggaac
     7021 tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca cggcgtgttg
     7081 ttctccagca ggatcactag atcgaaacgg tattcatcaa tcagcgcctg cacgaacgga
     7141 tgctcgcgtc cctcgtactt tttgcagaac gcctgagtgg tgacaaaatc ggtatcgata
     7201 aatgccactt tattggcata tttcaccgca aaatcaatgt attgagcgtg gcccagcgcg
     7261 attttatcgt agtcggaata ctgcaatgcg atctcatcac cgccgaggtg tgaaaagaca
     7321 taatcgcgac catattccca cgcactggtg gtattgaaga tattggcaag tttgtttacc
     7381 agggtggatt taccgctcga ctcgccgcca aggatcgcca cggtacgcac aaagaacggc
     7441 ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat ctgcgcaccg
     7501 ctgatactca taaaggtacg tttcggatcg accagcaccg tctcgatccc cagatgttcc
     7561 atatactgtg gcgcatcggc ttcttccgag gtgtagatca gatccggctg gatccctttt
     7621 tcagccataa actttttgat gccgttgctc cacacatccc agccgtgcgg atacggctcc
     7681 atgccctctt cgttgaaagc atgaatgcga atatttttct gatatttaaa agtttgcaat
     7741 aaccaacgca gacgatccgg cacggtcggc tgctgcgaca tggcactgtc ttcgaacaac
     7801 gcgcggtcac gggtatcgtc aaaacccata atgatatgca gctcgtcaac ctggctacag
     7861 gcgcgctgga taaggtagat atgtccggta tgcagtgggt agaacttacc gaatacgaca
     7921 ccgatcgttt tcttctgccg gggaaactca agccccaaaa aacggtgcaa cgcctccagc
     7981 ttttgcgcgc tggggctttt gattttggca ttcagtaact ggcttaaata ccctttggtc
     8041 ataccgctgg catcagctac ctgctgtagc gtgcagccct gttgcttgat ggcagttttc
     8101 aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg tcgaacacgc
     8161 taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc gcttttttcg
     8221 gcacgttagc cgccggaaca atcgcccggc gaaaaccgtg tttcgccgct tcagagattc
     8281 gttcctgacc gctgggcacc gggcggatct cccctgccag cccgacttca ccaaacacca
     8341 ccagatcctg cggcagcggt ctgtcgcgca ggctggaaac catcgccagc agtaacgcta
     8401 agtcggcact ggtttcggtt accttcacgc cgccgaccac gttcacaaac acatcctgat
     8461 cggccatttg caaaccaccg tgacggtgca agacagccag caggattgcc agacggtttt
     8521 gttccagccc cactgccacg cggcgcggat tcgccatcat cgagtgatcg accagcgcct
     8581 gaatctccac cagcaacgga cgcgttcctt cccacaccac catcacggag ctgccggagg
     8641 ttacttcatc gccgcggctt aagaagatag cggaagggtt gctaacttca cgcagcccct
     8701 gttcggtcat cgcgaagacg cccagctcat tcaccgcacc aaagcggttt ttatggctgc
     8761 gtaaggtgcg aaaacgggag tcggcatcgc catccaaaag taccgaacag tcgatacagt
     8821 gttccagcac tttcgggcca gccagcgaac catctttggt tacgtgcccc accatgacaa
     8881 tcgccacacc gcgcgttttg gcgaagcgtg tcaaataagc cgccgtttca cgcacctgcg
     8941 ccacgctacc aggcgatgac tgtacatccg ccatatgcat cacctggatc gagtcaatta
     9001 ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgttcgatg ctggtttccg
     9061 acaacatatt gagattgtca gtcggcaggc caaggcgatg agcgcgcatt gccacctgtt
     9121 gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgggccagt ttgcacagcg
     9181 tttgcagtag cagcgtggat ttccccgcac cagggttacc gccaatcaga atggcacttc
     9241 ctggcaccac gccgccgcct agtacgcggt cgaactcttt aaatccggtg gaaaaacgcg
     9301 gcagctcttc aaggctgata tcggagagtt tctggacttt tgccaccccg gcgctaccgg
     9361 catagccgct gagacgctcg ttacgcgcca ccgttggcga cgcagcaaga cgcacctcgg
     9421 tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga taatcggccc
     9481 cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacggtgtac ctcgttaatg
     9541 ctgtgccgcc cgcaggatgg cgggcgagca attacttctg attcaggctg cctgagagga
     9601 tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgccttt tcattcactt
     9661 ttggcttggc atggtaggca atccccagcc ctgccgcttt gatcatcggc aggtcattgg
     9721 ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc gcgaggcgag
     9781 tcagagtttt cgctttgtac tgcgcgtcta cgatgtcgcc gatcacattg ccggtaaatt
     9841 taccgtccat gatctccagt tcattggcta ccacggctgt caggcgcagc ttgtcgcgca
     9901 ggtattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag cccagcgttt
     9961 ccagcttgag taccagctgc gttaagcctg gcatcagcgg cagattttca cgcacctgtt
    10021 gcaaaatgtt cgcgtcagcg cctttcagtg tcgccacacg gctgcgcagg ctggcggtaa
    10081 aatcgagttc gccgcgcatc gcccgttcgg ttacctccgc caccatctcg cctgttccgg
    10141 ccagtttggc aatttcatca atacattcaa tctggatggc ggtggagtcc atgtccatca
    10201 caagcaaacc cggcgtgcgc aggtgcggga ttttccccag cggggcgaca tccagttgcg
    10261 cttcatgggc caggcgcgta gcccgcgcgg tgagtgaacc tgccagacga atcacctgat
    10321 aatcttccac gcaccaggcg gcaacaatca ccatcgccgc gcccagtttg ctctggtatt
    10381 gggtcagacg ttgtttatcc agtccacgac catacagcag ccagccgcta cgacctgcgt
    10441 ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagaccc ggccataaag
    10501 agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct gtaaaatcgt
    10561 tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg gcaacattaa
    10621 gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc ggctgcatcg
    10681 ggcagtgatt gtcctgttct gtcttgcctt gttagtggca ctgatgcagg gggcgtcatg
    10741 gtttagtcaa aaccaccagc gacagcgtaa tccacagctg gaagaactgg cccgcaccct
    10801 ggcgcgtcag gtgacgctga acgttgcacc gctgatgcgt accgactcac cggatgaaaa
    10861 acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgtatcctcg acgcgggtgt
    10921 gtatgacgaa caaggcgatc ttatcgcacg ttctggcgaa agcgtcgaag tgcgcgatcg
    10981 gctggcgctc gacggtaaaa aagcaggcgg ctattttaac cagcagattg tcgagccaat
    11041 tgcgggtaaa aacggaccgc tcggctatct gcgcctgaca ctcgacaccc atacgctcgc
    11101 caccgaagcc caacaggtgg ataacaccac taacatatta cgcctgatgt tgctgctctc
    11161 actggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgca cccgctggca
    11221 gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg gaagaggaag aaagcgagaa
    11281 aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac gcctgctcat
    11341 ctctgactct tacgacccgt ggtttaacct ggcggtggaa gagtgtattt ttcgccaaat
    11401 gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag taattggtcg
    11461 cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata acgtccgcct
    11521 ggcgcggcgc agtagcggtg gcggcgcggt gttccacgat ctcggcaata cctgctttac
    11581 ctttatggct ggcaagccgg agtacgataa aactatctcc acgtcgattg tgctcaatgc
    11641 gctgaacgcg ctcggcgtca gcgccgaagc gtccggacgt aacgatctgg tggtgaaaac
    11701 cgccgaaggc gaccgcaaag tctcaggctc ggcctatcgc gaaaccaaag atcgcggctt
    11761 ccaccacggc accttgctac tcaatgccga cctcagccgc ctggcaaact atctcaatcc
    11821 ggataaaaag aaactggcgg cgaaaggcat tacttcagtg cgttcccgcg tgaccaacct
    11881 caccgagctg ctgccgggga tcacccatga gcaggtttgc gaggccataa ccgaggcctt
    11941 tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc tccccggaca aaacgccaga
    12001 cttgccaaac ttcgccgaaa cctttgcccg tcagagtagc tgggagtgga acttcggtca
    12061 ggctccggca ttctcgcatc tgctggatga acgctttagc tggggcggcg tggaactgca
    12121 tttcgacgtt gaaaaaggcc atatcacccg cgcccaggta tttaccgaca gcctcaaccc
    12181 cgcgccgctg gaagccctcg ccgggcgact gcaaggctgc ctgtaccgcg cggatatgct
    12241 gcaacaagag tgcgaagcgc tgttggttga cttcccggac caggaaaaag agctacggga
    12301 gttgtcgacg tggatagcgg gggcggtaag gtaactcgct gattaaaata tccagggaag
    12361 gaaatatgat cgatctggaa aaccaggaac gggagattat caatttaatg ttcagccagc
    12421 gtatttcctg gctagccgcc gtgcgtatca ggcataaatt atcgcttgct gaagtaagta
    12481 aaatgttagg tatctcgatt aattctctaa aacagatcga aaagacggaa cggcttagct
    12541 caaacatcaa aagcaaaatg gcagaaattt atggttgtcc accagagttg ctgatatgcc
    12601 cttcctggat gacggctgaa cataaatgaa tagattcgtg aatagatact tgaatggatt
    12661 ttattttcta tccatatgaa aaataatagt aaatctataa atcagttgct attgatgaat
    12721 agatactaaa aagccggagc agtcccccac tccggcttaa cacgacaatc gcctttcagc
    12781 gaaacacaat tactctttat cgcccagcag aacggattcc agtgcgattt tgatcatgtc
    12841 gttgaaggtg gtctgacgct cagcggcagt ggtctgctcg tgagtgcgga tgtgatcaga
    12901 tacggtgcag atggtcaggg ctttcgcgcc aaattctgca gcgacgccgt agataccagc
    12961 cgcttccatt tccacgccga gaatgccgta tttttccata acgtcgaaca tttcgccgtc
    13021 cggagagtag aacaggtcag cggagaacag gttacccacg cgagcatcaa cgcccagtgc
    13081 tttagctgca tctactgcgt tacgcaccat gtcaaagtca gcgatagcgg caaagtcatg
    13141 atctttaaaa cggatgcggt taactttgga atcggtgcag gcacccatac cgataacgac
    13201 gtcgcgcagt tttacgtgcg gcagaactgc gccacaggaa ccaacgcgga taattttctt
    13261 cacgccgaaa tcggtgatca gttctttggt gtagatggag caggacggga tacccatacc
    13321 gtgacccatt acggaaattt tgcggccttt gtaagtacca gtgaagccca gcataccgcg
    13381 aacgttgttc acttcacggg catcttcaag gaaagtttca gcaatatact tcgcacgcag
    13441 cgggtcgcct ggcatcaaaa ctacgtcagc gaaatcgccc atttctgcat taatgtgtgg
    13501 ggtagccatt gttttatcct ttttgtgaca taacaaaggc ggagtcaacg ctccgcccaa
    13561 atccatcaga acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga
    13621 gtctggccga tatccgcgaa ggtttcacga tgacccagtg agcccggttt tactttcggg
    13681 ccatatacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatcg
    13741 caaccgtggt cagcggtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc
    13801 ggcagacggc ggtcgaacag ttccagaccc gcggcataac cggcgacgtc gcgacggtgg
    13861 ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct
    13921 ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cagtcgcttt cacttttttg
    13981 gtgataccgc agttggcgta gatgtccgca attttaccga cagaaaccac ctggccgtgt
    14041 ttttcatcaa ccagtttctg cagcacggta ggtgctggcg gctcaacagc caggtcgtga
    14101 cggttaccgg tacgctggaa gttaccggct ttgtcgccga taaacggacg agcgataaca
    14161 cgaccgatat tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag
    14221 agtttatcca gaccgaaagt ttcttcatga caggcaatct ggaacacgga gtcagcggag
    14281 gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagttgatc cagaatgacc
    14341 gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc gaccagttta
    14401 tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaacaga
    14461 accgggacac cggcaatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg
    14521 tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa
    14581 ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc
    14641 gggcctttac gaccgttatc agcttcgcct ttggcacaag cttctgcgat atgacccagg
    14701 gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag
    14761 tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa
    14821 atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc
    14881 ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg
    14941 ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaga
    15001 cgtgccatat cagtaaagcc gacgctgtaa tcgatggtgt cagatgcctg acggcgtccg
    15061 ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct
    15121 tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc
    15181 tcaacgaagt cggtcgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct
    15241 ttaccgttgt ccagcaccgc ctgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt
    15301 ttgccggaga taagcatctc cacacacagc gccatcgtga catcaaacag acgcgggtta
    15361 cgatattcac ccgtcaggaa ctgcaccgct tcacgtactt caaccgcgtt accggcactg
    15421 gaggccagca cctgattcat gtcggtgagc agtgcggtgg tgcgcacgcc tgcgccgtta
    15481 gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg
    15541 ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgccag tttcttcgcc
    15601 aggatcgagg cagtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg
    15661 tagaaacgtt tatccgccgg agccagtgag ctggtctgac cgataatcgc cacgccgacg
    15721 tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat
    15781 tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccggagat catcggaata
    15841 tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtgacatc gccgacgccg
    15901 ccggtcgagt gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg
    15961 gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg
    16021 aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaata
    16081 ccgttgataa agaaacgaat ttcttcatcg ctcagcgcat gaccatcacg ttttttacga
    16141 ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt
    16201 gtcggatgcg tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag
    16261 cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcgccg
    16321 tgacccagcg ctttcagcag gcttgccagc aggctggaag caccaaagcg gtagtgacgc
    16381 gcatcagccc agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct
    16441 tccgcagtac gcacgccgcc cgccggtttg aaaccaacgg ttttttctac gcccatatca
    16501 cggatcactt ccatcatgat gcgcgcgctt tccggcgtcg cgttcactgc cactttaccg
    16561 gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc
    16621 agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc
    16681 gcgcaagcct ctttacaggc tttcacaagg tcaaaaccaa cctgctcgtt acccgccatc
    16741 agcgcgcggt acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg
    16801 gtttctgcca gcgcgatttc gatgtcgtcg ttaccgtgtg ggaagttggt taccgtagcg
    16861 atacggattt ccggggtgcc ctgctctttc agagttttgc gagcaatcgg gataaagcga
    16921 ggatagatac agatagcggc ggtattgccg accggagttt tggcctgatg acacagggcg
    16981 atcactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca
    17041 cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt
    17101 caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg atacacatca
    17161 caattaagga aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt
    17221 cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga
    17281 aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca
    17341 tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga
    17401 gcagaagcgc cattttctgg cactggaagc agaaaacaat ctgccttacc cacagcttcc
    17461 tgccgaagcc cgccgcgcgc tggatgaagg tgtaatctgc gatatgtttg aaggtcatgc
    17521 gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga
    17581 atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct
    17641 ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt
    17701 gttgcaaccg tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt
    17761 ctggcgttac ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc
    17821 tgattcgccc attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc
    17881 gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc
    17941 gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat
    18001 tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg
    18061 tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga
    18121 tgacgtcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc
    18181 gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga
    18241 agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc
    18301 ggttaacttg ctgtgtgaaa aagaagggat tgccgcgcgc tacggtaaag aagccgccgc
    18361 aaatgaaata ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt
    18421 gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggatca gttccgatat
    18481 cggcaccacg ccgggcgcgc gtttaccgta tggcgatgag ccagatccga tcacccatct
    18541 gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca ttttgacgct
    18601 cgacgaaacc atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa
    18661 agccggaatg cgtgaattta ccgccaatgt cagcggtaac gatctggttc gcgttaccgg
    18721 ttatatggtg cgtttgtcag atttagaaaa atatcgcgcc gaaggttcac gcaccaacac
    18781 cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt
    18841 gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg
    18901 acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact
    18961 gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc
    19021 cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtctgcgagc
    19081 aatgtgatac ctgcctgaag aggtgtccgc aacatgccac gccaatggcg caatccatga
    19141 gcgtggacga agtgcttagc catgtccgca aagcagtgct gtttatcgaa gggataacgg
    19201 tgagtggcgg tgaagccacg acccagctgc cgtttgtggt ggcgctgttt actgctatca
    19261 aaaacgatcc gcaactgcgc cacctcacct gcctggtgga cagtaacggt atgttgagcg
    19321 aaaccggctg ggaaaaattg ctcccggtgt gcgacggcgc aatgctcgat ctcaaagcgt
    19381 gggggagcga atgtcatcaa caactcaccg gacgcgataa tcagcagatt aagcgcagca
    19441 tctgtttgct ggcagagcgc ggcaagctgg cggaactgcg tttgctggtg atccctggcc
    19501 aggtggatta tttgcaacac atcgaagaac tggcggtgtt tatcaaggga cttggcgatg
    19561 ttccggtacg cctgaacgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg
    19621 cgagcgccac gccggaagac gttgagccgt tggctgatgc gttacgggag cgcggggtga
    19681 gccggttaat atttccggct ctgtatttgt gacatgccgg at
//
LOCUS       DAPGRN010000092        19538 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPGRN010000092 DAPGRN010000000
VERSION     DAPGRN010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.092"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(390..766)
                     /gene="rnpB"
                     /locus_tag="RHW33_003392"
     ncRNA           complement(390..766)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RHW33_003392"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(799..1944)
                     /gene="garK"
                     /locus_tag="RHW33_003393"
     CDS             complement(799..1944)
                     /gene="garK"
                     /locus_tag="RHW33_003393"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV1646866.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVALDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2041..2931)
                     /gene="garR"
                     /locus_tag="RHW33_003394"
     CDS             complement(2041..2931)
                     /gene="garR"
                     /locus_tag="RHW33_003394"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV1646867.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2961..3731)
                     /gene="garL"
                     /locus_tag="RHW33_003395"
     CDS             complement(2961..3731)
                     /gene="garL"
                     /locus_tag="RHW33_003395"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV1646868.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSTSAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSISHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASVHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3747..5081)
                     /gene="garP"
                     /locus_tag="RHW33_003396"
     CDS             complement(3747..5081)
                     /gene="garP"
                     /locus_tag="RHW33_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV1646869.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5456..7027
                     /gene="garD"
                     /locus_tag="RHW33_003397"
     CDS             5456..7027
                     /gene="garD"
                     /locus_tag="RHW33_003397"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV1646870.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7176..7511
                     /gene="prlF"
                     /locus_tag="RHW33_003398"
     CDS             7176..7511
                     /gene="prlF"
                     /locus_tag="RHW33_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV1646871.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7511..7975
                     /gene="yhaV"
                     /locus_tag="RHW33_003399"
     CDS             7511..7975
                     /gene="yhaV"
                     /locus_tag="RHW33_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV1646872.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKILAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8030..8839)
                     /gene="agaR"
                     /locus_tag="RHW33_003400"
     CDS             complement(8030..8839)
                     /gene="agaR"
                     /locus_tag="RHW33_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV1646873.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9088..10368
                     /gene="kbaZ"
                     /locus_tag="RHW33_003401"
     CDS             9088..10368
                     /gene="kbaZ"
                     /locus_tag="RHW33_003401"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV1646874.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPNAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVESYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10391..10864
                     /gene="agaV"
                     /locus_tag="RHW33_003402"
     CDS             10391..10864
                     /gene="agaV"
                     /locus_tag="RHW33_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV1646875.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10875..11654
                     /locus_tag="RHW33_003403"
     CDS             10875..11654
                     /locus_tag="RHW33_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV1646876.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11644..12522
                     /locus_tag="RHW33_003404"
     CDS             11644..12522
                     /locus_tag="RHW33_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV1646877.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12540..12974
                     /locus_tag="RHW33_003405"
     CDS             12540..12974
                     /locus_tag="RHW33_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV1646878.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELSRCHEKCPVEEGI"
     gene            12971..14104
                     /gene="nagA"
                     /locus_tag="RHW33_003406"
     CDS             12971..14104
                     /gene="nagA"
                     /locus_tag="RHW33_003406"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV1646879.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDASDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGIDDVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14455..15609
                     /locus_tag="RHW33_003407"
     CDS             14455..15609
                     /locus_tag="RHW33_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV1646880.1"
                     /translation="MPENYTPAAAASGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFAVL
                     MPVETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15622..16482
                     /gene="kbaY"
                     /locus_tag="RHW33_003408"
     CDS             15622..16482
                     /gene="kbaY"
                     /locus_tag="RHW33_003408"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV1646881.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16649..17125
                     /gene="agaB"
                     /locus_tag="RHW33_003409"
     CDS             16649..17125
                     /gene="agaB"
                     /locus_tag="RHW33_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV1646882.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIGKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17164..17967
                     /gene="agaC"
                     /locus_tag="RHW33_003410"
     CDS             17164..17967
                     /gene="agaC"
                     /locus_tag="RHW33_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV1646883.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17957..18748
                     /gene="agaD"
                     /locus_tag="RHW33_003411"
     CDS             17957..18748
                     /gene="agaD"
                     /locus_tag="RHW33_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV1646884.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18749..19504
                     /locus_tag="RHW33_003412"
     CDS             18749..19504
                     /locus_tag="RHW33_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV1646885.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGENLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTSIPASFLWLHSNFICLINT"
BASE COUNT         4727 a         5082 c         4980 g         4749 t
ORIGIN      
        1 agacttaata agttcttaca aagtctatta ttaataccgt agatattcat gcatcttacc
       61 ctgtgcacca aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accacaaaaa
      121 tgcctgatgc gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg
      181 cttttgtagg caggctaagg cgttcacgcc gcatccggca tgaataaagc gcactcaaca
      241 atctgaaacc cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc
      301 tttattttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa
      361 acccgccgaa gcgggttttt acgtaaatca ggtgaaactg accgataagc cgggttctgt
      421 cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt
      481 tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt
      541 gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga
      601 tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc
      661 ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct ccgcccgtct
      721 cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag
      781 agggtttgcg cgcccttgtc accccgcgtt gcgcattcca atcgccagtg tcgcggcgat
      841 attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc
      901 tatgctggtc aatacgctga agaccgcatc aatgccatgc tgatgtacaa cgccaacatc
      961 atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc
     1021 gacaccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt caccggtgat
     1081 caccagcgta caatcgtgaa tatgttcctc cagattcagt gccgtagtga cgatttcaat
     1141 accacttttc agttccgcac caagaaacgc catcagcgcc gctcccatac cgccagcagc
     1201 tcctgcgccg gggacatctt tcacatcaac atgtagcgct tttttaatga cctcggcata
     1261 gtgagagagg ttattgtcca gcgcaacaat catcgcttca ctggctccct tttgtgggcc
     1321 aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac aagcgacgcg
     1381 aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat tcagagtatt
     1441 aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt tcgcccccag
     1501 cgcctgtacc atgcctgcgc cgccatcatt tgtagcgctg ccgccaatgc cgataataat
     1561 gtttgtcgcg ccgctctcca gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt
     1621 cacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg ccgccatttc
     1681 aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt tctcgcccag
     1741 cggccctgta acccaggcgt gacgttcgga accctgggtg gctgcaatca tcgcttccac
     1801 cgttccttcg ccaccgtcgg caacaggaac agaaacgtac tgtgcatcag gaaaaatttc
     1861 ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt ctttataaga
     1921 gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc ggagggcgtt
     1981 tctcttattc ggcctggatt ccaggcccgg attgcaatac gccatccggg cacgacgtca
     2041 ttaacgagta acttcaactt tcgccagttt ttcgtagtag cacgccaggg cgctatgatc
     2101 cgccgttcct aaaccatctg cacgcagtgc ctgcatcatc tccataaccg cagctgtgag
     2161 cggcagttgt gcgccgacgc cgtgagaagt atccagcgca ttcgccagat ccttaatatg
     2221 cagatcaata cggaagcccg gcttgaagtt gcggtccatc accatcggcg ctttggcatc
     2281 cagcacggta ctgcccgcca gtccaccgcg aattgcctga taaaccaggt ccgggttaac
     2341 gcccgcttta gtcgccagcg ttaacgcttc tgacatcgcg gcaatattca gcgccacaat
     2401 gacctgattt gccagtttgg tgacgttacc tgcaccgatt tccccggtat gcaccacgga
     2461 acccgccatc gctttcatca aatcatagta tttgtcgaaa atagccttgt cgccgcctac
     2521 catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg gagcatccag
     2581 catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca gcggtgcgat
     2641 agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc cattctcacc
     2701 cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga cgtcgcactg
     2761 ttcagcgatc gctttagccg tagacgctgt ttctgcacct gcagcaatca cgtcagcaat
     2821 agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa ggtttttact
     2881 cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca tatcaatcat
     2941 ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttctg agtggcagag
     3001 cggaagacgc cgagatcgct gccgacagcc acaaacgtcg cgccccattc cagataacga
     3061 cgcgcatcgg cttcgaccgg cgcgaggata ccgctgggtt tgccgtgcac gctggcacgg
     3121 ttaaaaatat gctgaattgc tttttgtaca tccgggtgtg aggcattgcc gagatggccc
     3181 aatgccgcag ccagatcgct gggaccgacg aagatgccgt ctacaccttc ggtagcggca
     3241 atggcatcga cgttatctac gccctgctga ctttctatct ggaccagaat agtgatgttc
     3301 ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg gtgagaaatg
     3361 gagacgccgc gaatgccttc tggcggataa cgcgtcgatg ccacagcctg ctctgcttcc
     3421 tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag aagacgctta
     3481 ataattaccg gctcgttggt cggcactcgc actactggcg cgctggtgct gcctttcagg
     3541 gccattaact gcggaataaa cgtggaaata tcgtttggcg catgttcgcc atccagcacc
     3601 agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga gagtgctgac
     3661 cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca
     3721 tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca
     3781 actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa aaccagtgct
     3841 gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt gacaatggag
     3901 gcaacattgc caaagacgtt aaagacgccg ccgcagaggc caacaatctc tttcggcgcg
     3961 gtgtcagaaa tcaccggcca gcccagcgca ccaaatcctt tgccaaagaa agccagcgcc
     4021 atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat ggtggaagcc
     4081 agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa accgcgtttg
     4141 atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca cagtgctgga
     4201 atcgaggcga ccagacccac tttcagaatc gacatacctt tttcctgcac cagataaatc
     4261 gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg
     4321 ccaagcatca tgcggttaga gagcaattgc ttgatgtaat gcagtttggg tccgcttgct
     4381 gccgcactgc ccggcttttt gtggtccata tcgaccaccg cgccattttc agagataaac
     4441 ttcagctctt ccgcagacat acgtgggtga tctgtcgggt tatgaatcaa tttgatccac
     4501 agcgccgtca gcacaaaacc aatccccccc ataacggtaa agacgtgctc ccagccccag
     4561 gcgaaagtca gccagccaag cagaggcgaa aagagcgcca gcgagaaata ttgcgccgag
     4621 ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc gacaatacgg
     4681 gcgttcgccg ggaatgatgg cgcttccgag aagccgagca taaagcgcat aaagaacatg
     4741 gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa ggtgaatagc
     4801 gaccagaaaa agaggctata ggtgtaaact tttttcgagc caaacttatc aagcagccag
     4861 ccgccgggga tttgcatcag caagtaggcc cagccaaaag cagaaaagat gtaacccatc
     4921 gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga cagcgttgca
     4981 cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa atagcgggta
     5041 tgcacgcctt tctttttttc gtcaacggtg tccagaatca ttttatttac ctcgggtact
     5101 tatgctgatt tttattatta tggggaaggt gttatttatg agtttcattt atgccgtaac
     5161 ggcaatgaac tcgggaatta gtataagcag cgcgagaata ataatcattg tgcaaatgct
     5221 aatttaatta attctattta aatattattt tgagcatatg cacataaggt tgcgcgctaa
     5281 agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctgaaat gattgacatt
     5341 gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa gtcatttatt
     5401 tttaaatgag accaggtcct cattttaata acccctggct ggagaatatt gcacaatggc
     5461 caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga
     5521 taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg
     5581 gctggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc
     5641 taatggtgaa attattcgtt atggcgaagt gattggttac gccgtgcgtg caatcccacg
     5701 cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct
     5761 gccactggca accaaagtcc cggaaccctt accgccgctg gaaggataca cctttgaggg
     5821 ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt
     5881 ccactgtgtg gcaggcgtgg tggactacgt agtaaaaatc attgaacgcg atctgctacc
     5941 gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggtt gtggcgtggc
     6001 gattaacgca ccggcggcag ttgttcctat tcgtaccatt cacaatattt cgctgaaccc
     6061 taactttggc ggcgaagtaa tggtgattgg cctgggttgt gaaaagttgc agcctgagcg
     6121 cctgctgact ggaacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag
     6181 tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc
     6241 cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact
     6301 ggttgtcggc atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg caaacccggc
     6361 ggttgggtat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tctcagaagt
     6421 cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg
     6481 caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga
     6541 ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaatg tagtggagaa
     6601 agcgctcggc tccattgcta aatcgggtaa aagtgcaatt gttgaagtgc tgtcgcccgg
     6661 tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg
     6721 tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac
     6781 gccgtacggc ctgatggcgg tacccgtcat taaaatggcg acccgcaccg agctggcgaa
     6841 ccgctggttt gatttaatgg atattaacgc gggcactatc gccaccggcg aagaaaccat
     6901 tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa
     6961 aaccttctcg gatcaatggg gactgcataa ccagctggcg gtgtttaacc cggcaccggt
     7021 gacctgattt cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg
     7081 tttaaagccc ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt
     7141 tgagcagaaa acaggctgta aaaggacagt gaatcatgcc cgctaatgct cgctctcacg
     7201 ctgtactgac cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcgccag
     7261 tgcgtgaggc tttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg
     7321 gtgggcaagt atttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat
     7381 ttttgcgttt tctggatgca gatatccaga acaacccgca aaaaactcgt ccattcaaca
     7441 ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa cattgatgat gagattggcg
     7501 acgacgaata atggattttc cacaaagggt taatggttgg gcgctatatg ctcatccctg
     7561 ttttcaggaa acctacgacg ctttagttgc cgaagtcgag acattaaagg gaaaagatcc
     7621 tgaaaattat cagagaaaag ccgccacaaa gatattggcg gtagtccata aagtgattga
     7681 ggagcatatc acggtcaatc catcatcacc ggcattccgt catggcaagt cgttaggctc
     7741 tgggaaaaat aaagactggt cacgggtaaa atttggtgct ggtcgttatc gtctcttctt
     7801 tcgttatagt gaaaaagaga aagtcatcat tctgggatgg atgaacgatg aaaacacgct
     7861 gcgcacctac ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt taaaaagagg
     7921 acatcctcct gccgactggg aaaccctcac ccgagagaca gaagaaaccc attgatggtg
     7981 ttcacaatgc ccgccgaacg cattcgtgtt gaccagcggg caccctttct cactccccga
     8041 ccagaatcac ttcaacccca gcctttcgca gcccttccag gctatccgca ggaatgcctt
     8101 catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat
     8161 tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac atccggcggt
     8221 ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta
     8281 caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt
     8341 aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg
     8401 cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtgct
     8461 tgcgcatcag acgagcaatc tcaaatgtgg tggtcccgga atcgaggatc acccgatgac
     8521 ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcattgcg gtgttcagcg
     8581 cgcttttatc ttccactgat ggctcgactg acggcgttgt gctatcgcag atcaacgcgc
     8641 caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatcg ttgcggatcg
     8701 tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc
     8761 gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc ttctcacctg
     8821 aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc
     8881 ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattgac gcaagctact
     8941 ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa ctattatctt
     9001 tcgttttatt tttatctcac catgacgcag tatcaagtga aacaaaacga aagattaata
     9061 tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac
     9121 aaagcgggca aaacaaatgg aatttatgcc gtttgttccg cacatccgct agtgctggag
     9181 gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat
     9241 caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt
     9301 tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt tgattctggg tggtgatcat
     9361 ctggggccaa accgctggca aaacctgccg gccgctcagg caatggccaa tgccgatgat
     9421 ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc
     9481 tgtcaggacg atccgattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg
     9541 gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg aagccgatct ggagtatgtc
     9601 attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc
     9661 accacgccga atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa
     9721 ggtttgaatg ccatctggcc acgcatcatt gctctggtgg ttcaacccgg cgtcgaattc
     9781 gatcacacca acgttattga ttatcagccc gccaaagcga ccgccttaag ccagatggtc
     9841 gaaagctacg aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatcg
     9901 ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc gctgaccttc
     9961 gccctgcgtg aagctctgtt ctctctggca gcgattgaag aagaactggt gccagcaaaa
    10021 gcctgttctg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc ggaatactgg
    10081 caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta tagctactcg
    10141 gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc tcatctggta
    10201 cgtaatctgg cggattcacc aattccgctg ccgctgatca gccagtatct gccgctgcag
    10261 tacgtgaaag ttcgctccgg cgagctgcag ccaacgccac gggaactcat tatcaaccat
    10321 attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga
    10381 ggaacacgct atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca
    10441 ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtactggtcg ccaacgatga
    10501 ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc
    10561 cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca
    10621 gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt
    10681 tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaagc aacaaatcgc
    10741 caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg
    10801 ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact
    10861 ttgagggatt catcatggaa atcagtctgt tgcaggcatt cgcgttgggc attatcgcct
    10921 ttatcgctgg cctggatatg tttaacggct taacccatat gcaccgccca gtggtcctcg
    10981 gcccgttagt cgggctggta cttggcgatc tgcataccgg tattttaacc ggcggtacgc
    11041 tggaactggt gtggatgggg ctggccccgc tggcgggcgc acagccgcct aatgtgatta
    11101 tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcag
    11161 tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg
    11221 tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca
    11281 ttgaacgcgt aaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt
    11341 tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac
    11401 gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc
    11461 tgctgaaaat catgatgaaa aacgtctaca tcccctactt catcctgggc tttgttgccg
    11521 ccgcctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg atggcgctga
    11581 tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg
    11641 aagatggcat ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga aacattgctg
    11701 actggcgtca atgaaaacgt gtatgaagat cagagcattg gcgcggagct gacgaaaaaa
    11761 gatatcaatc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt
    11821 atgcaggctt ccggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat
    11881 aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt
    11941 ctggtgacat ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac
    12001 agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg cgatgcaatg
    12061 ttctggctaa cgctactgcc gatttgtggc gggattggag ccagtctggc attgcaaggc
    12121 tccattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacacct ggggctgcgt
    12181 tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattccact cattaaagcg
    12241 aataccaaaa aagtcggcca tgctgcgtct atcgttggga tgacggtaat cggtgcgctg
    12301 gtggcaacct atgttcgttt aagcaccacg ctggaaatca cagcgggcga cgcagtggtt
    12361 aagttacagg ctgatgttat tgacaaactg atgcctgcct tcttaccgct ggtctatacc
    12421 ctgaccatgt tctggctggt acgtcgcggc tggagtccgc tacgcctgat tgcggttacc
    12481 gtggttctcg gcatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga
    12541 tgttaagtat tattttgaca gggcatggcg ggtttgccag cggcatggaa aaagcaatga
    12601 agcaaattct cggtgagcaa tcgcagttta tcgccatcga ttttccggaa acctcaagca
    12661 ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca
    12721 ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga
    12781 tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta ctggagatgg
    12841 tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac
    12901 atcgtgggct gaccagtctg gtggacgagt taagccgctg tcatgagaaa tgtccggtcg
    12961 aggaaggaat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggct
    13021 cgatgaccat cagttgcgta ttgctgacgg tgtaatcgca gcaatcgaac cgattccagt
    13081 gggcgtgact gaacgcgatg cggaactgct ctgccccgct tacatcgaca cccatgtaca
    13141 tggtggtgcg ggcgttgatg taatggatga cgcgtcggat gtactcgaca agctggcaat
    13201 gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg cgccgcttaa
    13261 taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc
    13321 gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca
    13381 tccgccggaa ttgtttcgcg agcttgaaat tgccgagctg gatcaattga ttgccgtttc
    13441 tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa gaaggggcat tgcaggccat
    13501 ccgccatctt aaacagcaaa atgtacgagt gatgctgggg catagcgcgg cgacctggca
    13561 acaaactcgc gccgcgtttg atgctggtgc cgacggcctg gtgcattgct acaacgggat
    13621 gacgggttta catcaccgcg aaccggggat ggttggcgcg ggattaacag acaagcgcgc
    13681 ctggctggag ctgatagccg atggtcatca tgtacatccg gcggcaatgt cgctgtgttg
    13741 ttgctgtgcg aaagagagaa tcgtactgat caccgacgcg atgcaggcag ccgggatgcc
    13801 ggatggtcgc tatacgttat gtggtgaaga agtgcaaatg cacggtggcg ttgtccgtac
    13861 cgcgtccggt gggctggcgg gcagtacgct gtctgtcgat gcggcagtgc gcaacatggt
    13921 cgagttaacg ggcgtaacgc ctgcggaagc catccatatg gcgtcgctgc atccggcgcg
    13981 aatgctgggt attgatgatg ttctgggatc gcttaaaccg ggcaaacgcg ccagcgtcgt
    14041 tgcgctggat agcgggctgc atgtgcaaca aatctggatt cagggtcaat tagcttcgtt
    14101 ttgatggttt gctcctttat tgggccttca cttcccccgt aaggcctttc tttttctttc
    14161 gttttgatct gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg acctacggta
    14221 cgccgcatcc ggcaatcggt gaacatagcg acgtcattac gcaattcttt aatcttccct
    14281 tgtcttttct ttcaatgatc acagatttcg ttttatttcc tttttctcca ttgaactttc
    14341 ggtttctttt ctatagattt taatcaacga aagacatcac caagtgaaat gaaacgaaag
    14401 gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg agttatgcca
    14461 gaaaattaca cccctgctgc cgccgcatcc ggtacatgga ctgaagaaga gatccgccat
    14521 cagcctcgcg catggatccg ttcactcacc aacatcgacg cgctacgttc cgcgctcaat
    14581 aacttccttg aaccgttact gcgcaaagag aatctgcgga tcatcctgac cggagccgga
    14641 acctcggcat ttatcggtga catcatcgcg ccgtggctcg ccagccatac cggtaaaaac
    14701 ttcagcgccg taccaaccac cgatctggtc actaatccga tggactacct gaacccagcc
    14761 catccgctgc tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgcagcc
    14821 gtggaactgg caaatcaatt tgtaccggaa tgctatcacc tgccgatcac ctgcaacgaa
    14881 gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg cgtttgccgt gctgatgccc
    14941 gtagaaacgc acgatcgcgg ctttgcgatg accagcagca ttaccaccat gatggccagc
    15001 tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa cctttcgcga cgtggcggat
    15061 cgttgccagg cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca
    15121 ccgtggaaac ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg
    15181 gcgctgaaag tgctggaact gacggcgggt aaactggcgg ccttctatga ttctccaacc
    15241 ggattccgtc atggaccaaa atcgctggtc gatgacgaaa cgctggtggt ggtatttgtc
    15301 tccagccacc cttacacccg tcagtatgat cttgatctgc tggctgaact tcgccgtgac
    15361 aaccaggcaa tgcgtgtaat cgccatcgcc gcggaaagca gcgacatcgt cgctgccggt
    15421 ccacatatca tcctgccacc gtcacgtcac tttatcgacg ttgagcaggc attttgcttc
    15481 ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc acatgggcaa tacgccggat
    15541 accccatcag ccagtggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg
    15601 caggcataag aggatcgcat tatgagcatt atctccacta aatatctgtt acaggacgcc
    15661 caggccaatg gctacgcggt gcctgctttt aacattcata acgccgagac gatccaggcg
    15721 atcctcgaag tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac gccggggacc
    15781 tttaaacata tcgcgctgga agagatctac gccctgtgca gcgcctattc cacaacctac
    15841 aacatgccac tggcgctgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa
    15901 gtccacgcag gtgtgcgcag tgcaatgatc gacggcagcc acttcccgtt tgccgagaac
    15961 gtgaagctgg tgaaatcggt tgttgacttc tgccactcac aggattgcag cgtggaagca
    16021 gaactgggcc gcctgggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc
    16081 ctgaccgatc cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg
    16141 gtagcgattg gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg
    16201 ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc cagcgatgtt
    16261 ccggatgaat ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca
    16321 gaattaaaaa tagccttcgc cggcgcggtt aaagcctggt ttgcggaaaa tccgcagggt
    16381 aatgatcctc gttattatat gcgcgtcgga atggatgcga tgaaagaagt tgtcagaaat
    16441 aaaattaatg tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat
    16501 ttatcccaat gtacttcccg attaatatcg gggagtgcct taatggaaaa ggagatgact
    16561 aaaccttaat aaatacatca gtacaatatc gcaacaataa tatatttaaa aaaattatat
    16621 tattcaactt tatggtgagg attacacaat gagcagtcca aatattctct taacccgtat
    16681 tgataaccgt ctggttcacg gtcaggttgg cgtgacctgg acatccacca tcggtgcgaa
    16741 tctgctggta gtcgtggatg atgttgtcgc taacgatgat attcaacaga aattaatggg
    16801 tattaccgca gaaacctacg gctttggcat tcgtttcttt actatcggaa aaaccattaa
    16861 cgtcatcggc aaagctgcac cgcatcagaa gatcttcctg atttgccgta cgccacaaac
    16921 ggtacgtaaa ttggtagaag gtggtattga cctgaaagat gtcaacgtcg gcaatatgca
    16981 tttctcggaa gggaaaaagc aaatcagcag taaagtttat gtcgatgacc aggatctcgc
    17041 ggacttacgt tttattaaac aacgtggcgt gaatgttttc attcaggacg tccctggcga
    17101 tcaaaaagaa caaatccctg actaaatcta aaatcgcctt aatattggtt tgaggtaata
    17161 aaaatgcatg aaataaccct acttcaggga ttatccctag cggcgttagt tttcgttctg
    17221 gggattgatt tttggctgga agccttattt ttattccgcc cgataatcgt ttgtacccta
    17281 actggcgcta ttctcggtga tattcagact ggcttaatta ccggtggtct gacagagttg
    17341 gctttcgccg gattaacccc tgcaggtggt gttcagccgc ccaaccctat tatggcgggt
    17401 ctgatgacca ccgtcattgc atggtctacg ggcgttgatg ccaaaacagc aattggtctt
    17461 ggcctgccgt ttagtttgtt aatgcagtac gtcattttgt tcttctattc cgctttctca
    17521 ttatttatga ccaaagccga taaatgcgcg aaagaggcgg acacggcagc gttttcccgg
    17581 cttaactgga caacgatgct catcgtcgct tcagcgtatg cggtgattgc tttcctctgt
    17641 acttacctgg cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc ctggttgacc
    17701 cacggctttg aagtggctgg cggtattctg cctgccgttg gttttggctt gctgctgcgc
    17761 gtgatgttca aagcacaata tatcccttac ctgatcgccg gtttcctgtt tgtttgctac
    17821 atccaggtca gcaacctgtt gccggttgcc gtactgggcg caggctttgc ggtgtatgag
    17881 tttttcaatg cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag taaaaatgaa
    17941 gaagaggact acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc gtctgggctt
    18001 tcgttcgtcg ctgctgcaag cgagctttaa ctacgaaagg atgcaggcgg gcggttttac
    18061 ctgggcgatg ttgccgatcc tgaaaaagat ttataaggac gacaaaccgg gcttaagcgc
    18121 ggcgatgaaa gataacctcg aatttattaa tacccacccg aatctggtcg gattcctgat
    18181 ggggttatta atttcgatgg aagaaaaagg agaaaaccgc gacaccatta aaggcctcaa
    18241 agtggcactg tttggcccaa tcgccgggat tggcgatgcg attttctggt ttactttgtt
    18301 gccgattatg gcgggaattt gctcatcatt tgccagccag ggaaacctgc tggggccgat
    18361 tctatttttc gccgtttacc tgcttatctt tttcctgcgc gtcggctgga cccacgtcgg
    18421 ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga tgattgcccg
    18481 ttcggcaacc atcctcggga tcacggtaat cggcgggctg atcgcttcgt atgtgcatat
    18541 taacgtggtg acgtcgtttg ccatcgacag tacccacagc gtcgcgctgc agcaggattt
    18601 cttcgataaa gtcttcccga acattttacc gatggcctac accctgctga tgtattactt
    18661 cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc gtgacttttg tgctctctat
    18721 tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc actgcgtctg gcggtgcctc
    18781 tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact atacggcgtt
    18841 aagtgaacgt gccagcgaat atttattggc cgtgatccgt agcaaaccgg atgccgtgat
    18901 ttgcctggcg accggagcca cgccattact gacgtatcat tatctggtag aaaaaatcca
    18961 ccagcagcag gttgatgtca gccagctgac cttcgtcaag ctcgacgaat gggtggatct
    19021 gccattaact atgcctggca cctgcgaaac tttcctgcaa cagcatatcg tgcagccgct
    19081 ggggctacgt gaagaccagc tcatcagctt tcgctccgaa gagataaatg agacagagtg
    19141 cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg ttctcggatt
    19201 ggggaaaaac ggtcatcttg ggctgaacga accgggagaa aacctgcaac cggcctgcca
    19261 tatcagtcaa cttgatgcca gaacacagca acatgagatg ttaaaaaccg cgggtcgccc
    19321 cgtgactcgt gggatcacct taggcctgaa ggatattctc aatgcccgcg aggttttgtt
    19381 actggtgact ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg ctaaagtctc
    19441 tacctctatc ccggcttcgt ttttgtggct gcacagtaac tttatttgtt taattaatac
    19501 ctaaccagat gaaaaatttg ctcaaaatgt ttaaatta
//
LOCUS       DAPGRN010000093        19006 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPGRN010000093 DAPGRN010000000
VERSION     DAPGRN010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.093"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..394
                     /locus_tag="RHW33_003413"
     CDS             8..394
                     /locus_tag="RHW33_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV1646886.1"
                     /translation="MALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINILFTSRQ
                     MIIETFICPVSSISNPDKFNTFLLRNQKMMPLSSVGISSVQQEAYYIVFGALSLKSSL
                     EDILLEITSLVDNALDLAEITEEYSH"
     gene            413..1111
                     /locus_tag="RHW33_003414"
     CDS             413..1111
                     /locus_tag="RHW33_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV1646887.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1162..1821
                     /locus_tag="RHW33_003415"
     CDS             1162..1821
                     /locus_tag="RHW33_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV1646888.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1839..2237
                     /locus_tag="RHW33_003416"
     CDS             1839..2237
                     /locus_tag="RHW33_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV1646889.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2247..2885
                     /locus_tag="RHW33_003417"
     CDS             2247..2885
                     /locus_tag="RHW33_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV1646890.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2888..4051
                     /locus_tag="RHW33_003418"
     CDS             2888..4051
                     /locus_tag="RHW33_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV1646891.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGKNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4135..5760
                     /locus_tag="RHW33_003419"
     CDS             4135..5760
                     /locus_tag="RHW33_003419"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV1646892.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAEHLEDGAYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5877..6152)
                     /gene="yjfN"
                     /locus_tag="RHW33_003420"
     CDS             complement(5877..6152)
                     /gene="yjfN"
                     /locus_tag="RHW33_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV1646893.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6301..6630)
                     /gene="bsmA"
                     /locus_tag="RHW33_003421"
     CDS             complement(6301..6630)
                     /gene="bsmA"
                     /locus_tag="RHW33_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV1646894.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6812..7561
                     /gene="yjfP"
                     /locus_tag="RHW33_003422"
     CDS             6812..7561
                     /gene="yjfP"
                     /locus_tag="RHW33_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV1646895.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAGEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7558..8313)
                     /gene="ulaR"
                     /locus_tag="RHW33_003423"
     CDS             complement(7558..8313)
                     /gene="ulaR"
                     /locus_tag="RHW33_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV1646896.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8421..9485)
                     /gene="ulaG"
                     /locus_tag="RHW33_003424"
     CDS             complement(8421..9485)
                     /gene="ulaG"
                     /locus_tag="RHW33_003424"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV1646897.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9840..11237
                     /gene="ulaA"
                     /locus_tag="RHW33_003425"
     CDS             9840..11237
                     /gene="ulaA"
                     /locus_tag="RHW33_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV1646898.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11253..11558
                     /gene="ulaB"
                     /locus_tag="RHW33_003426"
     CDS             11253..11558
                     /gene="ulaB"
                     /locus_tag="RHW33_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV1646899.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            11568..12032
                     /gene="ulaC"
                     /locus_tag="RHW33_003427"
     CDS             11568..12032
                     /gene="ulaC"
                     /locus_tag="RHW33_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV1646900.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDGVEQFGPYFVIAPGLAMPHGRPEEGVKKTGFSLVTLKKPLEFNHDDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12046..12696
                     /gene="ulaD"
                     /locus_tag="RHW33_003428"
     CDS             12046..12696
                     /gene="ulaD"
                     /locus_tag="RHW33_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV1646901.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12706..13560
                     /gene="ulaE"
                     /locus_tag="RHW33_003429"
     CDS             12706..13560
                     /gene="ulaE"
                     /locus_tag="RHW33_003429"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV1646902.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13560..14246
                     /gene="ulaF"
                     /locus_tag="RHW33_003430"
     CDS             13560..14246
                     /gene="ulaF"
                     /locus_tag="RHW33_003430"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV1646903.1"
                     /translation="MQKLKQQVFEANMELPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKAADMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14376..14651)
                     /locus_tag="RHW33_003431"
     CDS             complement(14376..14651)
                     /locus_tag="RHW33_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1646904.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14978..15373
                     /gene="rpsF"
                     /locus_tag="RHW33_003432"
     CDS             14978..15373
                     /gene="rpsF"
                     /locus_tag="RHW33_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV1646905.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15380..15694
                     /gene="priB"
                     /locus_tag="RHW33_003433"
     CDS             15380..15694
                     /gene="priB"
                     /locus_tag="RHW33_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV1646906.1"
                     /translation="MTNRLVLSGTVCRAPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15699..15926
                     /gene="rpsR"
                     /locus_tag="RHW33_003434"
     CDS             15699..15926
                     /gene="rpsR"
                     /locus_tag="RHW33_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV1646907.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15968..16417
                     /gene="rplI"
                     /locus_tag="RHW33_003435"
     CDS             15968..16417
                     /gene="rplI"
                     /locus_tag="RHW33_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV1646908.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16488..17282)
                     /locus_tag="RHW33_003436"
     CDS             complement(16488..17282)
                     /locus_tag="RHW33_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV1646909.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLAKKCNAYDGITMNGDNSFGWNHEATLDNIHAQKY
                     SKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVILPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSSS
                     AAIYSGLGFERVPQERDISCARMYLTPANHPDLWTQENGEWIYLRN"
     gene            17522..17824
                     /locus_tag="RHW33_003437"
                     /pseudo
     CDS             17522..17824
                     /locus_tag="RHW33_003437"
                     /inference="COORDINATES: protein motif:HMM:NF037007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17723..18337)
                     /gene="tnpA"
                     /locus_tag="RHW33_003438"
     CDS             complement(17723..18337)
                     /gene="tnpA"
                     /locus_tag="RHW33_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV1646910.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSKVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISAIRQYIEQQQTPGQVENRAVYPRPEGRGFT
                     AHRINQAKRMLVDQIISPKRSNINIHLKWVDIWLKNIVFLPFIQ"
     gene            18345..>19006
                     /locus_tag="RHW33_003439"
     CDS             18345..>19006
                     /locus_tag="RHW33_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1646911.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTENEVSTPVHPSALMVGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SR"
BASE COUNT         4606 a         4500 c         5031 g         4869 t
ORIGIN      
        1 gaatccgttg gcgctggcga cggcgctgca aactgtacct gaacaaaata ttgatgtaac
       61 aaatagcgag aacgcattaa ttattaaaat gaatgattat ggcgatttgc aaatcaatat
      121 tctttttact tcccgccaaa tgattatcga aacctttatt tgtccggtaa gtagtatcag
      181 caatccagat aaatttaata ccttcttatt aagaaatcag aagatgatgc cgttgtcatc
      241 ggtaggaatc tccagcgtgc aacaggaagc gtattacatt gttttcgggg cgttatcgct
      301 taaatcttct cttgaagata tcctgcttga gataacctcg ctggtagata acgcattgga
      361 tctggctgaa attacagaag aatattcaca ctaattacaa ggacgggtat ttatgggaat
      421 tttaaaaagt ttatttacgc tggggaaatc gtttatctcc caggcggaag aatccattga
      481 agaaacccag ggtgtgcgca tgctggaaca gcatattcgt gacgctaaag ctgaactcga
      541 taaagccgga aaatctcgcg ttgatctgct ggcgcgggtg aaattaagtc acgataagct
      601 gaaagattta cgtgagcgca aagccagtct ggaagcccgt gcgctggaag cgttaagcaa
      661 gaacgttaat ccgtcgctga ttaacgaagt tgctgaagaa atcgcgcgcc ttgagaatct
      721 cattaccgct gaagagcaag tgttgtcgaa tctggaagtc tcccgtgatg gcgtggaaaa
      781 agcagttaca gcgacagcgc agcgtatcgc tcagtttgag cagcaaatgg aagtcgttaa
      841 agccactgaa gccatgcagc gtgcacaaca ggctgtaaca acctctaccg ttggcgcatc
      901 ttccagcgtt tcgacagcgg cagaatcctt aaaacgcctg caaacgcgtc aggcagaacg
      961 tcaggctcgc ctggatgctg ccgcacagtt ggagaaagtc gcagacggtc gcgaccttga
     1021 cgaaaagctg gcggaagccg gaattggcgg tagcaataaa agtagcgccc aggatgtatt
     1081 agcaagactg caacgccaac agggcgagta atttttttgc cagcctcact gcaaggctgg
     1141 caactatttt aaggataaaa tatgtctggt tttttccagc gtctgtttgg caaggataat
     1201 aagcctgcta tcgctcgcgg tccgctggga cttcatctca atagtggctt tacgctcgac
     1261 acgttagcgt ttcgtttgct ggaagatgaa ttgctgattg cgctgccggg tgaggaattt
     1321 acggtagccg ctgtcagtca catcgatctg ggcggcggta gccagatttt ccgctactac
     1381 acttcaggcg atgagtttct gcaaatcaat accaccggcg gcgaagatat tgatgacatt
     1441 gatgatatca agctctttgt ctatgaagag agctacggta tcagtaaaga gagtcactgg
     1501 cgcgaggcga tcaacgccaa aacgatgggg gcaatgacct taaactggca ggaaaaacgc
     1561 tggcagcgat tttttaacag cgaagaaccg ggaaatatcg aacccgtcta catgctggaa
     1621 aaagtagaaa atcaaaatca tgctaaatgg gaagtccata attttaccat gggctaccag
     1681 cggcaagtga ccgaagatac ttacgaatat ttgctgctaa atggtgagga atcttttaac
     1741 gatctcggcg agccagagtg gttattttcg cgcgcgttgg gtgtcgatat cccgctgaca
     1801 tcacttcata ttattggtta attacaagga cgtttacaat gcatatactg gattcacttc
     1861 ttgcctttag tgcctatttt tttattggcg tggcgatggt gattattttt ctgtttatct
     1921 actccaaaat tacaccgcac aatgaatggc aattaatcaa aaacaataat accgcagcgt
     1981 cactggcgtt cagcggtaca ttgttgggtt acgtgatccc cttatccagt gcggcaatca
     2041 atgcggtgag tattccagac tatttcgcct ggggcgggat tgcactggtg attcagttac
     2101 tcgtttttgc tggtgtcaga ctttatatgc ccgcattaag cgaaaaaatt attaatcaca
     2161 ataccgcagc aggaatgttt atgggaaccg ccgcgctggc tggcggtatt tttaacgcag
     2221 cttgtatgac atggtaatgg acgatcatgg ccagaaaacg caaatcaaga aataacagta
     2281 aaattggtca cggagcgatc agtcgcattg gtagaccgaa taatcctttt gaaccgcgtc
     2341 gcaatcgcta cgcacaaaaa tacttaacgt tggcgctaat gggcggtgcc gctttttttg
     2401 tattgaaagg ttgtggcgat agtggcgatg tcgataacga cggcgacgga acgttttatt
     2461 cgacagtgca ggattgtatt gatgacggta ataatgccga catttgcgcg cgtggctgga
     2521 acaacgccaa agcggcattt tatgccgatg ttccgaagaa tatgactcag cagaactgtc
     2581 agtctaagta cgaaaattgc tactacgaca atgttgaaca gagttggatc ccggtcgttt
     2641 ctggattttt gttaagccgg gttattcgta aagatcgcga tgagccgttc gtctataaca
     2701 gcggcggttc ctcttttgct tcgcgccccg tctggcgcaa cacttctggt gattactcct
     2761 ggcgctccgg ttctggcaaa aaagagtctt actcttcagg cggctttacc accaaaaaag
     2821 cgtcaaccgt ttctcgcggc ggctatggtc gttcttccag cgcccgtggg cattggggag
     2881 gctaatcatg ctgagacaca acgttcctgt gcgacgggat ctggaccaga tcgccgctga
     2941 caacggtttc gactttcata tcatcgacaa tgaaatctat tgggatgaga gtcgggctta
     3001 ccgttttact ctgcgccaga ttgaagagca gatcgaaaaa ccgactgcgg aactgcatca
     3061 gatgtgcctt gaggtggtgg atcgcgcggt aaaagatgaa gagatcctga cgcaactggc
     3121 gatcccgccg ttgtactggg atgtgatcgc tgaaagctgg cgtgcccgcg atccttcgct
     3181 gtatggacgg atggattttg cctggtgtgg caatgcgccg gtgaagctgc tggagtacaa
     3241 cgccgatacg ccaacttcat tgtacgagtc ggcttatttc cagtggctgt ggctggagga
     3301 tgcccggcgc agcggcatta ttccgcgtga tgccgatcag tacaatgcta ttcaggaacg
     3361 cctgatttca cgctttagcg agctttacag tcgggaaccg ttttattttt gctgctgtca
     3421 ggacaccgat gaagacagga gtaccgtgct gtacttgcag gactgcgccc agcaggcagg
     3481 gcaggagtcg cggtttatct acattgaaga tctcggtttg ggcgtcggcg gcgtactgac
     3541 cgatcttgat gataatgtca tccagcgtgc atttaagctg tatccgctgg agtggatgat
     3601 gcgtgacgat aacggtccgc tgctgcgcaa acgtcgtgag caatgggtgg aaccgttatg
     3661 gaaaagtatt ttgagcaaca aagggttaat gccgctgctt tggcgcttct ttcctggtca
     3721 tcctaatctt cttgcgtcct ggttcgatgg cgagaaaccg cagattgccg ctggcgaaag
     3781 ctatgtgcgt aaaccaatct attcgcgcga aggcggcaac gtcaccattt ttgacggcaa
     3841 gaataacgtt gttgaccacg ctgatggtga ttacgccgat gaaccgatga tctaccaggc
     3901 gtttcaaccg ctaccgcgat ttggcgatag ttatacgctg attggtagct ggattgtcga
     3961 tgatgaagcg tgcggtatgg gtatccgcga agataacacg ctgatcacta aagacacttc
     4021 gcgttttgtt ccgcattaca ttgctggcta agaatgtttt agcaatctct ttctgtcagg
     4081 aatccgtggc agtgaccata ctaatggtga ctgccattga tggagggaga cacagtgcac
     4141 tggcaaactc acaccgtttt taatcaacct ataccattaa ataacagcaa cttatacctg
     4201 tctgatggcg cgctctgcga agcggtaacg cgtgaaggtg ctggctggga tagcgatttt
     4261 ctagccagta ttggtcagca gttaggaacg gctgaatccc ttgaactggg gcggctggcg
     4321 aatgtgaatc cgcctgaatt attgcgctac gatgcgcaag gacgccgtct ggacgatgtg
     4381 cgttttcacc ccgcctggca cctgctgatg caggcgctat gtaccaatcg ggtgcacaat
     4441 cttgcctggg aagaagacgc tcgctccggc gcatttgtgg cgcgcgcggc gcgttttatg
     4501 ttgcacgcac aggttgaggc tgggtcgtta tgtccaataa cgatgacctt tgccgccacg
     4561 ccattgttgt tacagatgtt acccgcgccg tttcaggact ggaccacgcc gctgttgagc
     4621 gatcgctacg attctcactt attgccaggt gggcaaaaac gcggtttgtt gattggcatg
     4681 ggaatgacgg aaaagcaggg cggttccgat gtcatgagca acaccacccg cgcagagcat
     4741 ctggaagatg gcgcttatcg gctggtgggg cataaatggt ttttctcggt tccgcaaagc
     4801 gatgcgcatc tggtgctggc gcagaccacg ggcggtctgt cctgtttttt tgtgccgcgc
     4861 tttttgcctg acgggcaacg caacgcgatt cgcctcgaac ggctgaaaga taagctgggt
     4921 aatcgctcta acgccagttg tgaagtggag tttcaggatg ccattggctg gttattgggg
     4981 caggaagggg aaggaattcg tctgatcctg aaaatggggg ggatgacgcg ttttgattgt
     5041 gccctgggta gccatgccat gatgcgccgt gcgttctcgc tggcgattta tcatgcacat
     5101 caacgccatg tttttggtaa tccattgatc caacagcccc ttatgcgtca tgtcttaagt
     5161 cgcatggcac ttcagcttga agggcaaacg gcgttgctgt ttcgtcttgc gcgagcgtgg
     5221 gaccggcgcg ccgatgccaa agaagctctg tgggcgcgtt tatttacgcc tgcggcaaaa
     5281 tttgtgatct gcaaaagagg tatgccgttt gtggccgaag cgatggaggt gctgggcggc
     5341 attggttatt gcgaggagag cgagttgccg cggctttacc gggagatgcc ggtaaacagt
     5401 atttgggaag gttccggcaa tattatgtgc ctggatgtgc tgcgcgttct caataagcaa
     5461 gcgggcgtat acgacttatt gtcggaagca tttgtggaag tgaaagggca ggatcgctat
     5521 tttgatcgcg cggttcgtcg tttacagcag cagctgcgta agccagctga agaactgggg
     5581 cgagagatta ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgcc
     5641 tctccgccaa tggcgcaggc gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg
     5701 ttgtcagagc agatccagaa tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa
     5761 gcgtatacga ctgatgcgac gctggtttcg attaactcaa tgaaatatgt gaaaattgta
     5821 ggccggacaa ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga tgacactcat
     5881 gcatacagta tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta catggtaata
     5941 atgcggtacc atgacgcacc ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc
     6001 tgcggatccc ctgaaatatt gctaactgag atctgaccta tttcattcag gcccgtcacg
     6061 cagtcagcac tggcgaattc tgcagactgt gccgtggcgc tcgcaggata agtcactaat
     6121 tgcagcgagg gtgaggcaag taattgtttc atcgtcagct ccatttttcg tttccccaca
     6181 tgttgcaggg cattccatcg cggtaataaa cgcccaggct tccagccggt aatttattgt
     6241 gcacaggtaa acgcataggg acgcaaagca atgtaaagct gactatgtaa gttgtctgat
     6301 ttatttacgg tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg tttcgtccac
     6361 catgacgaca acgtaataat cagcttttgc agcgacagct ttcgctctga tttctgctaa
     6421 tgcgtcatcc ggagaacccc gaaccatcgt gcttacgcta cctattcgct gtaacccttg
     6481 cgtctggtcg cggcgaatct cttgcggatg gtccgttact ggcggtgctg gctgtggcgt
     6541 accttgcagt gcgctacagg cacttaacat caacaccaat aataaactgg caaaccggta
     6601 aataacgcta ttacgtttcc tgctaaccat agtgtagtgc cttattaatt taactttggg
     6661 ggaatgttcc cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt
     6721 aacccacatc tcaacattat gaactaatac cgccagcaga aacttgtgct aaggatttct
     6781 tcatgtcata ccagacaaaa aggagagaca gatgattgaa atagaatcac gcgagctggc
     6841 agatattccc gttcttcatg cttatcctgt cggacaaaaa gataccccgt taccgtgcgt
     6901 gattttttat cacggcttta cttcgtccag tctggtgtat agctattttg ccgttgcgct
     6961 ggcgcaggct ggtttgcggg tgatcatgcc ggatgcgccc gatcacggta gccgttttag
     7021 tggtgacgca gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga
     7081 attcactact ttacgtgcgg caatagccgg agaaaactgg ctgcgtgatg accgtctggc
     7141 agtcggtggt gcgtcgatgg gcgcgatgac ggcactgggg attaccgctc gccaccccac
     7201 ggtgagatgt accgccagca tgatgggatc gggctatttt acatcactcg cccgttcact
     7261 gtttccaccg ctgatacctg aaacggcagc acagcagaat gaattcaata acattgtcgc
     7321 gccactggca gagtgggaag cgacaaacca cctggaacaa cttagtgaca gacctctact
     7381 gctgtggcat ggcctcgacg atgacgttgt gcctgccgac gaatcactac gtttgcagca
     7441 ggccttaagc gagacgggac gggataaact gctaacctgt tcatggcagc caggcgtgcg
     7501 tcaccgcatt acgcctgagg cgttagatgc tgccgtgaca tttttccgcc agcatcttta
     7561 aacacgcaga atgctgaccc cttgcgcttc cagttgttgc aggatttccg ggttagcatt
     7621 tttgccggtg ataagcatat cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc
     7681 gccaatctta ctgctatcaa ccagtaccac cagtttccct accacgctca gcatcttctg
     7741 ctctgccatt gctgtcagca tatcggtttt atacaaccct tctgcggtca gcccttttcc
     7801 gctggtaaac atccagtgcc cggcatagag actgttttcg ctgccctgcg ggcttaaagt
     7861 gatggactga cttttgttgt actgtccacc cataatgatc acgctgtcat gttcttgatc
     7921 gatcaggtaa tttgccagcg gtagataatt agtgatgatt tgcactggct tgccacacat
     7981 ttcccgcccg agcagaaatg cggtagagcc gcagttgatg accacgctct cgcccggatt
     8041 gaccagctgt gatgccgctt tggcgatacg tactttttca tcgtgattct gcgcctgatg
     8101 cagattcatc ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac
     8161 ttttttcagt ttgccgcttt cgtcaagttt attgatatcg cgtcgcgcag tggcaggcga
     8221 aattcccaga cgctcaacga ctttctcaac ggtcacaaag cccaattgtg cgagcatttc
     8281 caggagaatt tgatgtcttt gtgcttcagt catgagctat tccgataaaa tttgattttt
     8341 ttagatgata tttgaaatag ccaggaaata ctacgccgga tagcgcgaac ttctccacgc
     8401 tatccggtat aaacgacaga ttacaggaat gacttgaacg gcagatccgg ttcaatagtg
     8461 aagcaatcat cgaaaccgcg cggatagtgg tactcgaagt tgtctttatc cagcggccag
     8521 gtaaatttgc cgcccacctg ccagataaac ggcttaaagc catatttcag gcgatctttt
     8581 ttcatctccc acagcacgcg gatctcttgc ggatcggcct ggaagtttga ccagatatcg
     8641 tggtggaacg ggatcactac tttcgcattc agcgcttcgc ccatacgcag catatcggcg
     8701 ctggtcattt tgtcggtgat accgcgcggg ttttcgccgt acgatcctaa cgccacgtcg
     8761 atctgatgtt cgttaccgtg cttcgcataa tagttagagt agtgggagtc gccgctgtga
     8821 tacagcgaac cgcccggcgt tttgaacagg tagtttactg cgcgatcgtc catgccgtct
     8881 ggcagcacgc ctgccgcttt ttgatccgca ggcagggtaa tcagtgcagt acggtcgaag
     8941 gcgtcaagcg catgaatttc aatgtctttg actttcacca cgtcgcccgg tttaaccacg
     9001 atgcaacgtt ctttcggcac gccccagccg atccataaat ccacgcaggt tttcggtccg
     9061 ataaacggta cgtcatcagc gcagttctgc atcacggcag cagcgacgtt tacgtcgata
     9121 tgatcgttgt gatcgtgagt cgccaggacg gcgtcgatct ggcgaatagc aaatggatca
     9181 agaacaaacg gcgtagtgcg caggttcggc tgcagttttt tcacgccagc catacgctgc
     9241 atctggtgac cctgtttcat cagcgggtta ccgtgacttt gtttgccagt accgcaccaa
     9301 aaatcgacgc agacgttggt accgccttcc gatttcagcc agatcccggt acaaccgagc
     9361 caccacatcg cgaatgtacc aggagctacc tgctcttgtt caatttcttc attcagccag
     9421 ctaccccact ccgggaaagt gctcaggatc caggattcac gggtaatact tttcacttta
     9481 ctcatcgcca tttaccttca tgatagttca attcgaatca atatgtgatt ggttttgatt
     9541 aatcctgaca ctattttttc aggaaggcaa tgaccatttt ttgacttttt gccagggaag
     9601 ttgttgttga ttttgagtat ggaaagattt aatggaatgt gtaattcgat taactaaatg
     9661 aatttaaatg aataattgtt tcgttgtgtg aatcccactc tatccatgtg gaattatttg
     9721 cgggtcgcgt cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt
     9781 gagtgcacaa cattccgggt gtgtggaata cccggttacc tcttcttcag gagatcgtta
     9841 tggagatcct ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt
     9901 tgttgctggg tattgtgacc tgtctgggct acatcctact gcgcaaaagt gtcagcgtta
     9961 ttattaaagg cacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca
    10021 tcctcaccag caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg
    10081 gcgcaatttc agatacttac gcttcgatga tggcaaccat cgaccgcatg ggcgatgcct
    10141 atagctgggt aggttacgcg gtgttgttag cgctggcact gaacatctgt tacgtgctgc
    10201 tgcgtcgcat taccggtatt cgtaccatca tgctgaccgg ccacattatg tttcagcagg
    10261 cggggctgat tgccgtcacg ctgttcatct ttggctactc catgtggacc accattatct
    10321 gcaccgcgat tctggtttcg ctctattggg gcattacctc caacatgatg tacaagccga
    10381 ctcaggaagt caccgacggc tgtggtttct ccatcggtca ccagcaacag tttgcatcat
    10441 ggattgccta taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca
    10501 aactgccagg ctggctgaat attttccacg acaacatcgt ctccacggcg attgtcatga
    10561 ccatcttctt tggtgccatt ctgctctcct tcggtatcga caccgtgcag gcgatggcag
    10621 gcaaagtgca ctggacggtc tatatcctgc aaaccggttt ctccttcgcg gtggcgatct
    10681 tcatcatcac tcagggcgtg cgcatgtttg tggcggaact ctctgaagca tttaacggta
    10741 tctcccagcg cctgatccca ggtgcggttc tggcgattga ctgtgcggct atctatagct
    10801 tcgcgccgaa cgccgtggtc tggggcttta tgtggggcac catcggtcag ctgattgcgg
    10861 ttggcatcct ggtcgcctgc ggctcctcga tcctgattat tcctggcttt atcccgatgt
    10921 tcttctctaa cgccaccatc ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc
    10981 tgaagatttg tctggtgatg gggatgatcg aaatctttgg ttgcgtctgg gcggtgaaac
    11041 ttaccggtat gagtgcctgg atgggtatgg cggactggtc gattctggca ccgccgatga
    11101 tgcagggctt cttctccatc ggtatcgcct ttatggccgt catcattgta attgcactgg
    11161 cttatatgtt cttcgctggc cgcgcgctgc gcgcagaaga agatgcagaa aaacaactgg
    11221 cagaacagtc tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg
    11281 gcaacggaca aggcagttcc atgatcatga agatgaaagt ggaccagttt ttaacccaat
    11341 caaacattga ccacacggta aacagctgcg cggttggcga atacaaaagc gagttgagcg
    11401 gcgcggatat catcatcgct tctacgcaca ttgcgggcga aatcaccgtg accggcaaca
    11461 aatacgtggt tggcgtgcgc aacatgctct ctcctgctga ctttggcccg aaactgctgg
    11521 aagtgatcaa agagcatttc ccgcaggatg tgaagtaagg acgcgccatg aaattacgtg
    11581 attcgctggc ggaaaataaa tccatccgcc tgcaggctga agcagagaca tggcaggacg
    11641 cagtgaaaat cggcgttgac ctgctggtgg cggcggatgt tgtcgagccg cgctattacc
    11701 aggcgattct ggatggcgtg gaacagtttg gcccttattt cgttatcgct ccggggctgg
    11761 cgatgccgca cgggcggccg gaagagggtg ttaagaaaac aggtttctct ctggtaacgc
    11821 tgaaaaagcc gctggaattt aaccacgacg acaacgatcc ggtggatatc ctcatcacca
    11881 tggcggcggt cgatgccaat actcaccagg aagtgggcat catgcagatc gtcaacctgt
    11941 ttgaagatga agagaatttt gaccgtttac gcgcctgccg taccgagcag gaagtactgg
    12001 atctcattga ccgcaccaac gcggcagctt aagaaggaat tgaacatgtc attaccgatg
    12061 ttgcaagtcg cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt
    12121 gccgaagaag tcgacattat cgaagtgggc accattctgt gcgtgggcga aggcgtgcgt
    12181 gcggttcgtg acctgaaagc gctctacccg cacaaaatcg tactggcaga cgccaaaatt
    12241 gccgatgcag gcaaaatcct ttcgcgtatg tgcttcgaag ccaacgctga ctgggtgacg
    12301 gtaatttgct gtgcggatat caacaccgcc aaaggcgcgc tggacgtggc aaaagagttt
    12361 aacggcgacg tgcagatcga actgaccggt tactggacct gggaacaggc gcagcagtgg
    12421 cgtgacgcag gcattcagca ggtggtttat caccgcagcc gtgacgcaca ggccgcaggc
    12481 gtggcgtggg gtgaagcgga cattaccgcg atcaaacgtc tttccgatat gggcttcaaa
    12541 gtcaccgtca ccggaggcct ggcgctggaa gatctgccgc tgttcaaggg tattccgatt
    12601 cacgtcttta tcgcgggccg tagtatccgt gatgccgctt ctccggtgga agccgcacgt
    12661 cagttcaaac gttccatcgc tgaactgtgg ggctaaggag cggatatgtt gtccaaacaa
    12721 atcccgcttg gcatctatga aaaagcgctc cccgccgggg agtgctggct ggaacgcctg
    12781 caactggcaa aaacgttggg cttcgatttt gtcgagatgt cggtagatga aacggacgag
    12841 cgcctgtcgc gcctcgactg gagccgcgag cagcgtctgg cgctggtcaa tgcgattgtt
    12901 gaaaccggcg tgcgcgtgcc gtccatgtgc ctttctgctc atcgtcgttt cccgctgggc
    12961 agtgaagatg acgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc tatccagttc
    13021 gcccaggacg ttggtattcg cgtgatccag ctggcgggct atgacgttta ttatcaggaa
    13081 gccaataacg aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggca
    13141 agccgtgcgc aggtgacgct ggcgatggag atcatggatt atccgttgat gaactccatc
    13201 agcaaggcgc tgggctacgc gcactatctc aacaacccgt ggttccagct ctacccggat
    13261 atcggcaatc tgtcggcatg ggacaacgac gtgcagatgg agttgcaggc cggaatcggg
    13321 catatcgtcg cggtgcatgt gaaagacacc aaaccgggcg tcttcaaaaa cgtaccgttt
    13381 ggcgaaggtg tagtggattt cgaacgttgt ttcgaaacgc tcaaacagag tggctattgc
    13441 gggccgtacc tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtggcg
    13501 aaagcgcgtg attgggtgaa agcgcgcatg gccaaagcgg gcatggtgga ggcggcataa
    13561 tgcaaaagct aaaacagcag gtatttgaag ccaacatgga gctgccgcgc tacgggctgg
    13621 tgacctttac ctggggcaac gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca
    13681 agcccagcgg cgttgcctac gaaaccatga aagcggccga tatggtggtg gttgatatga
    13741 gcggcaaggt ggtggaaggg gagtatcgcc catcttccga cactgcgacg catctcgaac
    13801 tctaccgtcg ttacccgtcg cttggcggca ttgtccatac ccactccact catgccaccg
    13861 catgggcgca ggcggggctg gcgatcccgg cgttaggcac cacgcacgct gactacttct
    13921 ttggtgatat cccgtgtacg cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac
    13981 tgaacaccgg caaagtgatt atcgaaacgc tgggcaacgc cgagccgctg catacgccgg
    14041 gaattgtggt gtatcagcac gggccgttcg cctgggggaa agatgctcac gatgcggtgc
    14101 ataacgcggt ggtgatggaa gaagtggcga aaatggcgtg gattgcgcgc ggcattaacc
    14161 cacaactgaa tcacatcgac agctatctca tgaacaaaca cttcatgcgt aagcacggtc
    14221 ccaacgccta ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cttcgggaga
    14281 tgactggtct gattccgcag ccgtttttca aaaaaatgcc cccggagaag ggggcaaagc
    14341 aaactatggc aatgtttcgt tggttatacc tggtgctagc gataaatatc cgcgctggcg
    14401 tgcatattgc cgttactccc cggttcccgc atcagaatca cgtggtagta cgttgcgcct
    14461 tgcgcatctg tctcttcatt taatgcctga cgtgcttcac tttcagtggc gaagttatgg
    14521 ttgatataaa tcacgcctaa gctctgtacg tcgtccatat ttctggcctg gtggttatta
    14581 atttcaatgg ctgcccatgt atttgcactt agcaaaagca cagccagaag ggctaaaaca
    14641 cgactgaaca tagatacctc ctcgacggct gactttgtgt gctctcctct gtgatgatct
    14701 tctgatttaa ttttaatcaa tgataaagaa gttgatggtg accatttctg atgcagctgt
    14761 tcaaaaaaac accatgatga agtgtgatga acttcaaatc agcgtgttag tacgctttgg
    14821 tgaaagatga gatttatttg tcggctggcc ttgagtttag cggggtatgt cagtactata
    14881 acgcgtcatt tttcagccga cctttaacac gttccttgcc tccccgggat tcggctgacc
    14941 cagacaggag gctgaataat ccgtaaggag caattcgatg cgtcattacg aaatcgtttt
    15001 tatggtccat cctgaccaga gcgaacaggt tccgggcatg atcgagcgct acactgctgc
    15061 catcactggt gcagaaggca agatccaccg tctggaagac tggggccgcc gtcagctggc
    15121 ttacccgatc aacaaactgc acaaagctca ctacgttctg atgaacgttg aagctccgca
    15181 ggaagtgatc gatgagctgg aaactacctt ccgcttcaac gatgccgtta tccgcagcat
    15241 ggttatgcgt accaagcacg ctgttaccga agcatctccg atggttaaag cgaaagacga
    15301 gcgccgtgag cgtcgcgatg atttcgcaaa cgaaaccgct gatgatgctg aagctgggga
    15361 ttctgaagag taatttctga tgaccaaccg tctggtgttg tccggcaccg tgtgcagggc
    15421 tccccttcga aaggtcagtc catcaggaat tcctcactgc cagttcgtgc ttgagcatcg
    15481 ttctgtgcag gaggaagccg gttttcaccg gcaggcgtgg tgccaaatgc ccgttattgt
    15541 tagcggacac gaaaaccagg ccattactca cagtataacg gtcggcagtc gcataaccgt
    15601 tcaggggttc atttcatgcc acaaggcaaa gaacggtctg agcaaaatgg ttttgcatgc
    15661 cgagcagatt gaattgatag attctggaga ctagccatat ggcacgttat ttccgtcgtc
    15721 gcaagttctg ccgtttcacc gcggaaggcg ttcaagagat cgactataaa gatatcgcta
    15781 cgctgaaaaa ctacatcacc gaaagcggta agattgtccc aagccgtatc accggtaccc
    15841 gtgcaaaata ccagcgtcag ctggctcgcg ctatcaaacg cgctcgctac ctgtccctgc
    15901 tgccgtacac tgatcgccat cagtaatcgg tcacggtcca ttaatacgac tttgagagga
    15961 taaggtaatg caagttattc tgcttgataa agtagcaaac ctgggtagcc tgggtgatca
    16021 ggtaaacgtt aaagcgggct atgctcgtaa cttcctggta ccgcagggta aagctgttcc
    16081 agctaccaag aaaaacattg aattcttcga agcacgtcgc gctgaactgg aagctaaact
    16141 ggctgaagtt ctggcagcag ctaatgctcg cgctgagaaa atcaatgcac tggaaactgt
    16201 taccatcgcg tctaaagctg gcgacgaagg taaactgttc ggttccatcg gtactcgcga
    16261 catcgctgac gctgtaactg cagctggcgt tgaagtggct aagagcgaag ttcgtctgcc
    16321 gaacggcgtt ctgcgtacca ctggcgaaca cgaagtgagc ttccaggttc acagcgaagt
    16381 attcgcgaaa gtgatcgtaa acgtagtagc tgaataattc gttattcaac gagacgtaaa
    16441 aacgccgacc agtggtcggc gttttgcttt ctatttttcg tcaggtatta gtttcgcaag
    16501 tagatccatt caccattctc ttgtgtccat aagtcaggat ggtttgctgg cgttagatac
    16561 atacgagcgc aactgatgtc tcgctcctgc ggcactcgct cgaaacccaa acccgagtag
    16621 attgcggctg aacttgataa ggaacgcaga ccaattttca aatcatgggg taaatcagaa
    16681 tacgcgttta gcaatgcagc aatgatgaat ccgagggcat tagaaacgcc tgtaacagtg
    16741 cgaagttggt cgatataaag atcgattgtt ggtagttcag atgaccagca attaggcatt
    16801 tgggcaggaa ggattacaac gtatcctatc gtttggccgg tatagtctgg agtaccataa
    16861 ccaggtccca aaaccacttc gtgtattgtg tatttactgt tgggttcgaa ggattcgaca
    16921 tcttgcaata aatatttcag ttctgcggtt aacctcgcac ccgcgtaagc tttactgtat
    16981 ttctgggcgt gaatattatc taatgtagct tcatggttcc atccaaaaga attatcacca
    17041 ttcattgtga ttccatcgta agcattacac ttctttgcta acttattgaa tgcttgccag
    17101 tctgagagat ctagtttgtg gatctcaaca gtacgtctgc catcgcaatt atgatctctt
    17161 ccacgatttg acagtaatgt agcgcgtgcg tattgatgaa attttagttt tgctggtttg
    17221 tcggtggacg aaaaacgagg taaatatgcg gggaatgaat aaatagttgt tggtaatgtc
    17281 attttattgt tgcctgttta atatttttac aaatatcgca gttgaatgtt caaagaaaaa
    17341 gaattgatat taataaaaat aagtttgttt tctgaagtag tgaaaatagc ggatggtgaa
    17401 agatattaat atcaaaaatc gctcattgtg gatataacga tgcagcaggg gagaaaatga
    17461 cttccacgcc ttacaccgta cattcttgtt gtgcggtatg taattattct acgctgtcgg
    17521 tattggtgta ttcgttaatc agcaggttaa ccagccacaa aaagccattg agaaaattat
    17581 tgattttaca tggaattatt atattgccaa tccttggttt ttaaaaattg tgcatagtga
    17641 aaatcaaagt aaaggtgtgc attatgcaaa atcacagcgt ctcttagaaa ttacccatgc
    17701 gcacttgcag ttaatggaat ccttattgga tgaagggaag aaacacaata tttttaagcc
    17761 agatatcgac ccatttcagg tgaatattaa tattgctgcg cttgggggat attatttgat
    17821 caaccagcat acgcttggcc tggtttatcc ggtgcgccgt aaaaccccgt ccttcagggc
    17881 ggggatatac ggcgcggttt tccacctgac caggtgtttg ctgttgttca atgtattgac
    17941 ggatggcgga tattggcgca cctccgcagc tactggcaaa atagccagga ctccacaaaa
    18001 cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt aacctacccg
    18061 atacgccttt gaggctgttt accagactgg atatcgccag tttgggagga tagttaatta
    18121 acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca aaatcagcac
    18181 atacctttga aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa atctggcgtc
    18241 ggtatctggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc cttccacgcc
    18301 ggatatctgt ttcttttttc acagaccaaa gtatcatttg gcctgtgaaa cgactacagg
    18361 catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg cgcttcgcag
    18421 gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat catgaggccg
    18481 gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca
    18541 ctgaaacgca atggcttaaa gatgccccgt cacagccatt gcaacagtca ctgaaagacc
    18601 ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga ttcaagaagc
    18661 ggggacagaa tgatgcattc cgctacccgc agggcgttaa gctcgatcag gaaaacagcc
    18721 gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag gtcacgggtg
    18781 ttgtgaaaaa tgtcactgtc agccagtcat gcggtaagtg gtacatcagt attcagactg
    18841 aaaatgaagt atcaactccg gttcaccctt cagcattaat ggtcggactg gatgctggcg
    18901 tggctaaact cgccacgctg tcagatggca cagtctttgg gcctgtaaac agttttcaga
    18961 aaaaccagaa gacgctggcg agacttcagc gacagttaag ccgcaa
//
LOCUS       DAPGRN010000094        18766 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPGRN010000094 DAPGRN010000000
VERSION     DAPGRN010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.094"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            84..533
                     /gene="alaE"
                     /locus_tag="RHW33_003440"
     CDS             84..533
                     /gene="alaE"
                     /locus_tag="RHW33_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV1646912.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(570..914)
                     /locus_tag="RHW33_003441"
     CDS             complement(570..914)
                     /locus_tag="RHW33_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV1646913.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAR"
     gene            1066..1395
                     /locus_tag="RHW33_003442"
     CDS             1066..1395
                     /locus_tag="RHW33_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV1646914.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1643..1888
                     /gene="nrdH"
                     /locus_tag="RHW33_003443"
     CDS             1643..1888
                     /gene="nrdH"
                     /locus_tag="RHW33_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV1646915.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1885..2295
                     /gene="nrdI"
                     /locus_tag="RHW33_003444"
     CDS             1885..2295
                     /gene="nrdI"
                     /locus_tag="RHW33_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV1646916.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2268..4412
                     /gene="nrdE"
                     /locus_tag="RHW33_003445"
     CDS             2268..4412
                     /gene="nrdE"
                     /locus_tag="RHW33_003445"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV1646917.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYPRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYNENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTHEMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4422..5381
                     /gene="nrdF"
                     /locus_tag="RHW33_003446"
     CDS             4422..5381
                     /gene="nrdF"
                     /locus_tag="RHW33_003446"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV1646918.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRSDEPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTEELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5737..6939
                     /gene="proV"
                     /locus_tag="RHW33_003447"
     CDS             5737..6939
                     /gene="proV"
                     /locus_tag="RHW33_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV1646919.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6932..7996
                     /gene="proW"
                     /locus_tag="RHW33_003448"
     CDS             6932..7996
                     /gene="proW"
                     /locus_tag="RHW33_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV1646920.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8053..9045
                     /gene="proX"
                     /locus_tag="RHW33_003449"
     CDS             8053..9045
                     /gene="proX"
                     /locus_tag="RHW33_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV1646921.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9237..10421
                     /locus_tag="RHW33_003450"
     CDS             9237..10421
                     /locus_tag="RHW33_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1646922.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10545..11282
                     /gene="ygaZ"
                     /locus_tag="RHW33_003451"
     CDS             10545..11282
                     /gene="ygaZ"
                     /locus_tag="RHW33_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV1646923.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11272..11607
                     /gene="ygaH"
                     /locus_tag="RHW33_003452"
     CDS             11272..11607
                     /gene="ygaH"
                     /locus_tag="RHW33_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV1646924.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11698..12228
                     /gene="emrR"
                     /locus_tag="RHW33_003453"
     CDS             11698..12228
                     /gene="emrR"
                     /locus_tag="RHW33_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV1646925.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12355..13527
                     /gene="emrA"
                     /locus_tag="RHW33_003454"
     CDS             12355..13527
                     /gene="emrA"
                     /locus_tag="RHW33_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV1646926.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13544..15082
                     /gene="emrB"
                     /locus_tag="RHW33_003455"
     CDS             13544..15082
                     /gene="emrB"
                     /locus_tag="RHW33_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV1646927.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15146..15661)
                     /gene="luxS"
                     /locus_tag="RHW33_003456"
     CDS             complement(15146..15661)
                     /gene="luxS"
                     /locus_tag="RHW33_003456"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV1646928.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(15811..17367)
                     /gene="gshA"
                     /locus_tag="RHW33_003457"
     CDS             complement(15811..17367)
                     /gene="gshA"
                     /locus_tag="RHW33_003457"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV1646929.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17440..17868)
                     /locus_tag="RHW33_003458"
     CDS             complement(17440..17868)
                     /locus_tag="RHW33_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV1646930.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(17865..18431)
                     /gene="yqaB"
                     /locus_tag="RHW33_003459"
     CDS             complement(17865..18431)
                     /gene="yqaB"
                     /locus_tag="RHW33_003459"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV1646931.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4440 a         4874 c         4770 g         4682 t
ORIGIN      
        1 tatgcgaaaa tgcagatggc aatgagatcc actgctttca tctccattaa catcccatta
       61 cgcttttatt aaggagcatt agcatgttct caccgcagtc acgcttgcgt catgcagttg
      121 cagatacgtt cgcgatggtt gtttactgtt ctgtcgtgaa catgtgtatt gaagttttcc
      181 tctccggaat gagcttcgaa cagtcttttt attccagatt ggtagcgatt ccggtgaaca
      241 tcttaattgc atggccatac ggtatgtacc gcgatctgtt tatgcgcgcg gcacgcaaag
      301 ttagcccgtc gggctggata aaaaatctgg cggatatcct ggcttatgtg acgttccagt
      361 caccggtgta tgtggcgatc ttgttagtgg tgggcgcaga ctggcatcag attatggcgg
      421 cggtcagttc aaacatcgtt gtttcgatgt tgatgggggc ggtttatggc tacttcctcg
      481 attattgccg ccgactgttt aaagtcagcc gttaccagca ggtaaaagcc tgactcttct
      541 tttcgcgact ggcatcgcca gtcgcgtctt taacgggctt cgccaaataa accattcaaa
      601 taacgttcaa gcgcaacacg cgaactaaag ccatgcggga taccgtaatg ctcgtgtcgc
      661 tcgcctgcta aatagagcgg aaattgctgg taatgatcgc aagttttaat atcggaagcg
      721 ggttcagcca gtgtcgaact gcgttctttt aatgttggat gaattaccag agcggtacgt
      781 cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta accatacgct
      841 ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc cacctcgtct
      901 ggtcgtaaat acatatttga tcctcgttat tatcaaccgc gggcctacct tacctgattg
      961 cgcattcaca acgctttcag aaaagtccat aaagccgcga tgaacctcag ttaagaaaat
     1021 tatggtctac actgaaaatt acatcgaatt ctaatggggg atcatatgtt taaccgaccg
     1081 aaccgcaacg acgttgatga tggcgtgcag gatatccaga atgatgtcaa tcaattagct
     1141 gacagcctcg aatctgtatt gaagtcctgg ggcagcgacg ccaaagggga agctgaagcc
     1201 gcacgcagta aagctcaggc attgctgaaa gaaaccaggg cgcgaatgca tggtcgtact
     1261 cgcgtccagc aagccgcacg cgatgccgtt ggctgcgcgg attcttttgt tcgtgaaaga
     1321 ccctggtgta gtgtgggaac tgctgctgcg gtaggtattt ttatcggtgc gttgttgagc
     1381 atgcgtaaat cgtagtgcaa aaatgataat aaatacgcgt ctttgacccc gaagcctgtc
     1441 ttcggggttt ctttttacct ggtgaatcac aaaaatcccc ctaccccgtc acgctcatat
     1501 ccagggtaat ttcgaccact atttgctata tattgtgtgg ttgaatcttt tttcaactac
     1561 atctagtatc tctgtatcaa cagagagaca acccgacgcg tatcatcgcg ccgtatcttc
     1621 attttaaacg gaaatacgaa tcatgcgcat tactatttac actcgtaacg attgcgttca
     1681 gtgccacgcc accaaacggg cgatggaaaa ccggggcttt gattttgaaa tgattaatgt
     1741 cgatcgcgtt cctgaagcgg cagaagcgtt gcgtgctcag ggctttcgtc agttgccggt
     1801 agtgattgct ggcgatctta gctggtctgg tttccgtccg gacatgatta accgtctgca
     1861 tccagcgcca cacgcggcca gtgcatgagc cagctcgtct acttctccag cagctccgaa
     1921 aacacgcagc gttttatcga acgtttaggt ctgcccgcgg tgcgcatccc gctcaatgag
     1981 cgggaacgga ttcaggtaga cgagccttac atcctgatcg tgccctctta cggcggcggc
     2041 ggtacggctg gcgcggtgcc acgacaggta attcgctttt taaacgacga gcataaccgg
     2101 gcattgctgc gcggcgttat tgcttcgggt aatcgcaact ttggtgaggc gtatggccgc
     2161 gccggagatg tgattgcccg gaaatgcggc gtgccgtggc tgtaccgttt tgaactcatg
     2221 ggtacgcaaa gcgatatcga aaacgttcgt aaaggagtaa ccgaattttg gcaacgacaa
     2281 ccgcagaacg cctgacgcag gaaacgatgg attatcacgc gctgaatgcg atgcttaacc
     2341 tctacgatag cgcaggtcgc attcagttcg ataaagaccg ccaggccgtt gacgccttta
     2401 ttgcgacgca tgtgcgtccg aacagtgtga ccttcagtag ccagcagcag cgcctgaact
     2461 ggctggtcaa cgaaggttac tatgatgaaa gcgttcttaa tcgctaccct cgcgactttg
     2521 tcattacgct gtttgcccac gcacacacca gcggttttcg tttccagaca ttcctcgggg
     2581 catggaagtt ttacaccagc tatacgttga agacattcga cggtaaacgt tatctggaag
     2641 attttgccga tcgagtcacg atggtggcgc tgacgctggc acaaggcgat gaaacactgg
     2701 cgacgcagct cactgatgag atgctgtcag gacgctttca gccagccacg ccgacattcc
     2761 tcaactgcgg taagcagcag cgcggcgaac tggtttcctg ttttctgctg cgtattgaag
     2821 acaatatgga gtcgattggc cgggcggtaa attccgcact acaactgtcg aaacgcggcg
     2881 gcggcgtggc atttttgctg tcgaatctgc gagaagcggg cgcgccaatt aaacgtattg
     2941 aaaatcaatc ttctggcgta attccggtga tgaaaatgct ggaagacgca ttttcctatg
     3001 ccaaccaact cggcgctcgt cagggggctg gtgcagtcta tttacatgct catcatcccg
     3061 atattctgcg ttttctcgac acgaaacggg aaaatgccga cgaaaaaatc cgcattaaaa
     3121 cactgtcgct gggcgtggtg atcccggata tcactttcca tctggcaaaa gagaatgcgc
     3181 agatggcgct gttttcgcct tatgacgtag agcgagttta tggcaagccg tttgccgatg
     3241 tggccatcag ccaacactat gacgaactgg ttgccgatga acgcattcgc aaaaaatacc
     3301 tcaacgcccg tgatttcttc cagcgactgg cagaaatcca gtttgagtcc ggctatccct
     3361 acatcatgta tgaagacacg gtaaaccgtg ctaaccctat cgccgggcgc ataaatatga
     3421 gtaatctctg ctcagagatt ttgcaggtta acagcgcctc agagtataac gagaatctcg
     3481 actatgcccg cacaggccat gatatctcct gcaatttagg ttcgttgaat attgcccaca
     3541 ccatggattc ccccgacttt gcccgcacgg tagagactgc cgtacgcggt ttaacggcgg
     3601 tgtcagatat gagtcatatc cgcagcgtgc cgtccatcga agccggaaat gccgcctcgc
     3661 acgccatcgg actggggcag atgaatttac atggttatct ggcgagggaa ggcatcgctt
     3721 atggttcgcc ggaagcactg gatttcacca atctctattt ctatgccatc acctggcacg
     3781 cactgcgtac ctcgatgttg ctggcacgcg aacgcggtga aaccttcgcc gggttcaaac
     3841 agtcacgcta tgccagtggt gaatatttta gccaatatct gcaagggaac tggcagccga
     3901 aaacggcgaa agttggcgaa ctgtttaccc gtagcggtat tacgttacct acccatgaga
     3961 tgtgggcgca gctgcgcgac gacgtgatgc gctacggcat ttacaaccag aatcttcagg
     4021 cggtgccgcc aaccggttct atctcttata tcaaccatgc tacgtcgagt attcatccga
     4081 ttgtggcgaa agtagagata cgcaaagagg gcaaaacagg acgcgtttac taccctgccc
     4141 cgtttatgac taacgaaaat ctggcgctat atcaggacgc ttacgaaatc ggcgcagaaa
     4201 agatcatcga cacctacgcg gaagcgactc gccatgtcga tcaggggctg tcgctgacgc
     4261 tgtttttccc cgataccgcc accactcgcg atatcaacaa agcgcagatt tacgcctggc
     4321 gcaaaggtat caaaacgctc tattacatcc gcctgcgtca gatggcgctg gaaggcactg
     4381 aaattgaagg ctgcgtctcc tgtgcacttt aaggaatatc tatgaaactc tcacgtatca
     4441 gcgccatcaa ctggaacaag atatctgacg ataaagatct ggaggtgtgg aatcgcctga
     4501 ccagcaattt ctggctacca gaaaaggtgc cactgtcgaa cgatatacct gcatggcaga
     4561 cattaagtgt cgtagaacaa caactgacaa tgcgcgtttt tactggcctg acgctgctcg
     4621 acacgctgca aaatgttatc ggcgcgcctt cgctgatgcc cgatgcgctc acgcctcatg
     4681 aagaggcagt attatcgaat atcagcttta tggaagcggt tcatgcccgc tcttacagtt
     4741 caattttctc gacgctgtgc cagaccaaag atgtcgatgc cgcctacgcc tggagtgaag
     4801 aaaacgcgcc gttgcagcga aaagcacaga ttattcagca acattatcgc agtgatgaac
     4861 cgctgaaaaa gaaaatcgcc agtgtgtttc ttgaatcttt tttgttctat tccggtttct
     4921 ggctgccgat gtatttttcc agccgcggaa agctaaccaa taccgcggac ctgatccgtc
     4981 tgattatccg cgatgaagca gtccacggtt actacatagg ctataaatat cagaaaaaca
     5041 tggaaaagat atctctggga caacgtgaag agttgaagag tttcgccttc gatttgttgc
     5101 tggaactcta cgacaacgag ttgcaataca ccgaagagct gtacgccgaa accccgtggg
     5161 ctgacgatgt gaaagcgttt ctctgttaca acgccaataa ggcgttgatg aatttgggct
     5221 acgaaccgct atttcccgca gaaatggcgg aagtgaatcc ggcaatcctc gccgcgcttt
     5281 cgccgaatgc cgatgaaaat cacgatttct tttccggttc aggctcttca tatgtgatgg
     5341 gaaaagcggt cgaaacagaa gatgaagact ggaatttctg agtgggttat tttcaaaaat
     5401 atcactaccc gctgcaggga aataattccc tccaaatagc tttatatcac gcaaataatt
     5461 tgtggtgatc tacactgata ctctgttgca ttattcgcct gaaaccacaa tattcaggcg
     5521 tttttttgct atctttgaca aaaaatatca acttttctcg atttgctctc agcccttata
     5581 tcatgggaaa ttccggcgat ttgctcacat caatattcat gccacatttg ccctcagggg
     5641 ttgcctcaga ttctcagtat gttagggtag aaaaaagtga ctatttccat tgggtaatgt
     5701 atcgacataa acaaataaca ggaatctttc tattgcatgg caattaaatt agaaattaaa
     5761 aatctttata aaatatttgg tgagcatcca cagcgagctt tcaaatatat cgaacaagga
     5821 ctttcaaaag aacaaattct ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt
     5881 ctggccattg aagaaggcga gatatttgtc atcatgggat tatccggttc gggtaaatcc
     5941 acaatggtac gccttctcaa tcgcctgatt gaacccaccc gcgggcaagt gctgattgat
     6001 ggtgtggata ttgccaaaat atccgacgcc gaactccgtg aggtgcgcag aaaaaagatt
     6061 gcgatggtct tccagtcctt tgccttaatg ccgcatatga ccgtgctgga caatactgcg
     6121 ttcggtatgg aactggccgg aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca
     6181 ctgcgtcagg tcgggctgga aaattatgcc cacagctacc cggatgaact ctctggcggg
     6241 atgcgtcaac gtgtgggatt agcccgcgcg ttagcgatta atccggatat attattaatg
     6301 gacgaagcct tctcggcact cgatccatta attcgcaccg agatgcagga tgagctggta
     6361 aaattacagg cgaaacatca gcgcaccatt gtctttattt cccacgatct cgatgaagcc
     6421 atgcgtattg gcgaccgaat tgccattatg caaaatggtg aagtggtaca ggtcggcaca
     6481 ccggatgaaa ttctcaataa tccggcgaat gattatgtcc gtaccttctt ccgtggcgtc
     6541 gatattagtc aggtattcag tgcgaaagat attgcccgcc ggacaccgaa tggcttaatt
     6601 cgtaaaaccc ctggcttcgg cccacgttcg gcgctgaaat tattgcagga tgaagatcgc
     6661 gaatatggct acgttatcga acgcggtaat aagtttgtcg gcgccgtgtc tatcgattcg
     6721 cttaaaaccg cgttaacgca acagcaaggt cttgatgcgg cgctgattga tgcgccgtta
     6781 gcggtcgatg cacaaacgcc tcttagcgag ttgctctctc atgtcggaca ggcaccctgt
     6841 gcggtgcccg tggtcgacga ggaccaacag tatgtcggca tcatttcgaa aggaatgctg
     6901 ctgcgcgctt tagatcgtga gggggtaaat aatggctgat caaaataatc cgtgggatac
     6961 cacgccagcg gcggacagtg ccgcgcaatc cgcagacgcc tggggtacac cggcgactgc
     7021 accgactgac ggcggtggtg ctgactggct gaccagtacg cctgcgccaa acgtcgagca
     7081 ttttaatatt ctcgatccgt tccataaaac gctgatcccg ctcgacagtt gggtcactga
     7141 agggatcgac tgggtcgtta cccatttccg tcccgtcttc cagggcgtgc gcgttccggt
     7201 tgattatatc ctcaacggtt tccagcaatt gctgctgggt atgcccgcac cggtggcgat
     7261 tatcgttttc gctctcatcg cctggcagat ttccggggtc ggaatgggtg tggcgacgct
     7321 ggtttcgctg attgccatcg gcgcaatcgg tgcctggtcg caggcaatgg tgactctggc
     7381 gctggtgtta accgccctgc tgttctgtat cgtcatcggt ttgccgttgg ggatctggct
     7441 ggcgagaagt ccgcgagcgg cgaaaattat tcgtccactg cttgatgcca tgcagaccac
     7501 tccagcgttt gtttatctgg tgccaatcgt catgctattt ggtatcggta acgtgccggg
     7561 cgtggtggtg acgatcatct ttgctctgcc gccgattatc cgtctgacca ttctggggat
     7621 taaccaggtt ccggcggatc tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca
     7681 gatgctgttc aaagttcagt taccgctggc gatgccgacc attatggcgg gcgttaacca
     7741 gacgctgatg ctggcccttt ctatggtggt catcgcctcg atgattgccg tcggcgggct
     7801 gggtcagatg gtacttcgcg gtatcggtcg tctggatatg gggcttgcca ccgttggcgg
     7861 cgtcgggatt gtgatcctcg ccattatcct cgatcgcctg acgcaggccg ttgggcgcga
     7921 ctcacgcagt cgcggcaacc gtcgctggta caccactggc cctgtcggtc tgctgacccg
     7981 cccattcatt aagtaactct gcacttgccc ggtgacgccg ggcatatcac cctgccaaaa
     8041 aaaggaataa caatgcgaca tagcgtactt tttgcgacag cgtttgccac gcttatctct
     8101 acacaaactt ttgctgccga tctgccgggc aaaggcatta ctgttaatcc agttcaaagc
     8161 accatcaccg aagaaacctt ccagacactg ctggtcagtc gtgcgctgga gaaattaggt
     8221 tataccgtca ataaacccag cgaagtggat tacaacgttg gctacacctc gcttgcttcc
     8281 ggcgatgcaa ccttcaccgc cgtgaactgg acgccactgc atgacaacat gtacgaagct
     8341 gccggtggcg ataagaaatt ttatcgtgaa ggggtatttg ttaacggcgc ggcacagggt
     8401 tacctgatcg ataagaaaac cgccgaccag tacaaaatca ccaacatcgc acaactaaaa
     8461 gatccgaaga tcgccaaact gttcgatacc aacggcgacg gtaaagcgga tttgactggc
     8521 tgtaaccctg gctggggctg cgaaggtgcg atcaaccacc agcttgccgc gtatgaactg
     8581 accaataccg tgacgcataa tcaggggaac tacgcggcga tgatggctga caccatcagt
     8641 cgttacaaag agggcaaacc ggtgttttac tacacctgga cgccgtactg ggtgagtaat
     8701 gagctgaagc cagggaaaga tgtggtctgg ttgcaggtgc cgttctctgc actgccgggc
     8761 gataaaaatg ccgataccaa actgccgaat ggcgcgaatt acggcttccc ggtcagcacc
     8821 atgcatatcg ttgccaataa agcctgggcc gagaaaaacc cggcagcagc gaaactgttt
     8881 gccattatgc agttgccagt ggcagatatt aacgcccaga acgccattat gcatgacggc
     8941 aaagcctcag aaggcgatat tcagggccat gttgatggct ggatcaaagc ccaccagcag
     9001 cagttcgatg gctgggtgaa tgaggcgctg gcagcgcaga agtaattttt attcgggcgg
     9061 attaggcgtt tttgcctcat ccgccgctct gtacacaatg cctgatgcga cgctaacgcg
     9121 tcctatcatg cctacaaacc acatcgcaca tttcaacaat ctatctttca tcccatattc
     9181 atcaacatcc gctattattg atttccagct taatcatcac ctgatgaaca aaaataatga
     9241 ctaaacctaa tcatgagctt agcccggcgc tgatcgtgct gatgtctatc gccaccggtc
     9301 tggcggtagc cagtaactat tacgcccagc cattgctcga caccatcgcg cgtaactttt
     9361 ccctttccgc cagttcggca ggctttattg ttaccgccgc gcagttgggc tatgccgcag
     9421 gtctactgtt tcttgttccc ctcggtgata tgtttgaacg ccgccgcctg attgtctcga
     9481 tgaccttact ggcagcgggc ggtatgttaa tcactgccag cagtcagtca ctggcgatga
     9541 tgatcctcgg tacggcatta accggtttat tctcggtcgt ggcacaaatt ctggttccgc
     9601 tggcagcgac gctggcttca ccggataaac gcggcaaagt ggtcggcacc attatgagcg
     9661 gtctgctgtt ggggatcttg ctggcacgaa ccgttgccgg gttactggca aacctcggcg
     9721 gctggcgcac cgtcttttgg gttgcctcgg tgttaatggc actgatggcg ctggcattat
     9781 ggcgtggtct gccacaaatg aaatcagaaa cccacctcaa ctacccacag ttgttgggtt
     9841 ccgttttcag catgtttatc agcgataaga tcctgcgcac ccgcgcgttg ctgggctgcc
     9901 tgacttttgc caacttcagc attctctgga cctcaatggc ctttttgctt gccgctccac
     9961 cttttaacta cagcgatggt gtaattggtc tgtttgggct tgcgggagct gccggagcgt
    10021 tgggcgctcg tccggcgggc ggttttgccg acaagggcaa atcgcaccac accacaacat
    10081 tcggtctgct tctgctgtta ctttcatggc tggcaatctg gtttggtcac acttccgtac
    10141 tggcgttgat tatcggcatt ctggtgctgg acctcaccgt acagggcgtg catatcacta
    10201 accagacggt aatttatcga atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca
    10261 tgaccagcta ctttattggc ggcgccgccg gttcgctaat ttcagcctca gcctggcaac
    10321 atggcggttg ggctggcgtt tgtctggctg gcgcgacgat tgccctggtt aacttactgg
    10381 tctggtggcg aggttttcat cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc
    10441 gtggctttac acttataagg gtgttaagaa gcccatcagt ctgataaggt taagatattc
    10501 attcagtcta tttataatat taacaatcgt taagcgtaca ctctatggaa agccctactc
    10561 cacagcctgc tcctggttcg gcgaccttca tggaaggatg caaagacagt ttaccgattg
    10621 ttattagtta tattccggtg gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct
    10681 ctcctctcga aagcgttttt ttctcctgca tcatttatgc aggcgcgagc cagttcgtca
    10741 ttaccgcgat gctggcagca gggagtagtt tgtgggttgc tgcactgacc gtcatggcaa
    10801 tggatgttcg ccatgttttg tatggcccgt cactgcgtag ccgtattatt cagcgtctgc
    10861 aaaaatcgaa aaccgcccta tgggcgtttg gcctgacgga tgaggttttt gccgccgcca
    10921 ccgcaaaact ggtacgcaat aatcgccgct ggagcgagaa ctggatgatc ggcattgcct
    10981 tcagttcatg gtcatcgtgg gtatttggta cggtaatagg ggcattctcc ggcagcggct
    11041 tgctgcaagg ttatcccgcc gttgaagcag cattaggttt tatgcttccg gcactcttta
    11101 tgagtttcct gctcgcctct ttccagcgca aacaatctct ttgcgttacc gcagcgttag
    11161 ttggtgccct tgcaggcgta acgctatttt ctattcccgt cgccattctg gcaggcattg
    11221 tctgtggctg cctcactgcg ttaatccagg cattctggca aggagcgccc gatgagctat
    11281 gaggttctgc tgcttgggtt actggttggc gcggcgaatt attgcttccg ctatttgccg
    11341 ctgcgcctgc gtgtgggtaa tgcccgccca accaaacgtg gcgcggtagg tattttgctc
    11401 gacaccattg gcatcgcctc gatatgcgct ctgctggttg tctctaccgc accagaagtg
    11461 atgcacgata cacgccgttt cgtgcccacg ctggtcggct tcgcggtact gggtgccagt
    11521 ttctataaaa cacgcagcat tatcatccca acactgctta gtgcgctggc ctatgggctc
    11581 gcctggaaag tgatggcgat tatataaccc acaagaatca tttttctaaa acaatacatt
    11641 tactttattt gtcactgtcg ttactatatc ggctgaaatt aatgaggtca tacccaaatg
    11701 gatagttcgt ttacgcccat tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat
    11761 tttccttatc aggagatcct tctgactcgt ctttgcatgc acatgcaaag caagctgctg
    11821 gagaaccgca ataaaatgct gaaggctcag gggattaacg agacgttgtt tatggcgttg
    11881 attacgctgg agtctcagga aaaccacagt attcagcctt ctgaattaag ttgtgctctt
    11941 ggatcatccc gtaccaacgc gacgcgtatt gccgatgaac tggaaaaacg cggttggatc
    12001 gaacgtcgtg aaagcgataa cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt
    12061 cacgagtttt tgcgcgaggt tttaccaccg cagcataact gcctgcatca actctggtcc
    12121 gcgctcagca caacagaaaa agatcagctc gagcaaatca cccgcaaatt gctctcccgt
    12181 ctcgaccaga tggaacaaga cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg
    12241 ctcaaaaatc cagatttata aaagaaaaaa tgactggcca gcatcgcaac atgctggcct
    12301 ttttggcaag caggtcggct cagccgatga gttaagaaga tcgtggagaa caatatgagc
    12361 gcaaatgcgg agactcaaac cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt
    12421 ctgctcctcc ttctcacctt gctctttata attattgccg tagcgatagg gatttattgg
    12481 tttttggtac tgcgtcactt cgaagaaacc gatgacgcat acgtggcagg gaatcaaatt
    12541 caaattatgt ctcaggtgtc tggcagcgtg acgaaagtct gggccgataa caccgatttt
    12601 gtaaaagaag gcgacgtgct ggtcactctc gacccgacag atgctcgcca ggcgtttgaa
    12661 aaagccaaaa ctgcactggc ttccagcgtt cgccaaaccc accagctgat gattaacagc
    12721 aaacagttgc aggcgaatat tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac
    12781 tacaaccgcc gtgtgccgct gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac
    12841 gcccgcgacg ccgttaccag tgcccaggcg caactggacg tcgcgattca acaatacaat
    12901 gccaatcagg cgatgattct ggggactaaa ctggaagatc agcctgccgt gcaacaggct
    12961 gccaccgaag tacgtaacgc ctggctggcg ctggagcgta ctcgtattgt cagtccgatg
    13021 accggttatg tctcccgccg cgcggtacag cctggggcgc aaattagccc aacgacgccg
    13081 ctgatggcgg tcgttccagc caccaatatg tgggtggatg ccaactttaa agagacgcag
    13141 attgccaata tgcgcatcgg tcagccggtc accatcacca ccgatattta cggcgatgat
    13201 gtgaaataca ccggtaaagt ggttggtctg gatatgggca caggtagcgc gttctcactg
    13261 cttccagcgc aaaatgcgac cggtaactgg atcaaagtcg ttcagcgtct gcctgtgcgt
    13321 atcgaactgg accagaaaca gctggagcaa tatccgctgc gtatcggttt gtccacgctg
    13381 gtgagcgtca ataccactaa ccgtgacggt caggtactgg caaataaagt acgttccaca
    13441 ccggttgcgg taagtaccgc gcgtgaaatc agcctggcac ctgtcaataa actgatcgat
    13501 gatatcgtaa aagctaacgc tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc
    13561 gctggaaggc gcgcaactgg tcattatgac gattgcgctg tcactggcga cattcatgca
    13621 ggtgctggac tccaccattg ctaacgtggc gatccccact atcgccggga atctgggctc
    13681 atcgctcagc cagggaacgt gggtaatcac ttctttcggg gtggcgaatg ccatctcgat
    13741 cccgcttacc ggctggctgg caaagcgcgt cggggaagtg aaactgttcc tctggtcaac
    13801 catcgccttt gctattgcgt cgtgggcgtg tggtgtctcc agcagcctga atatgctgat
    13861 cttcttccgc gtgattcagg ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct
    13921 attgctgaat aattacccgc cagccaaacg ctcaatcgcg ctggcgttgt ggtcgatgac
    13981 ggtgattgtc gcgccaattt gcggcccaat cctcggcggg tatatcagcg ataattacca
    14041 ctggggctgg atattcttca tcaacgtgcc gattggcgtg gcggtggtgt tgatgaccct
    14101 gcaaaccctg cgcggacgtg aaacccgcac cgaacggcgg cgaattgatg ccgtggggct
    14161 ggcactgctg gttattggta tcggcagcct gcagattatg ctcgaccgcg gtaaagagct
    14221 ggactggttt tcatcacagg aaattatcat ccttaccgtg gtagcggtgg tggctatctg
    14281 cttcctgatt gtctgggagc tgaccgacga taacccgata gtcgatctgt cgttgtttaa
    14341 gtcgcgcaac ttcaccatcg gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg
    14401 cgctattgtt ctgctgccgc agttgttgca ggaggtctac ggttacacgg ctacgtgggc
    14461 agggctggcc tctgcgccag tagggattat tccggtgatc ctgtcgccga ttatcggccg
    14521 cttcgcgcat aaactggata tgcgccgact ggtaaccttc agctttatta tgtatgccgt
    14581 ctgcttctac tggcgagcct atacctttga accaggtatg gattttggcg cgtcggcctg
    14641 gccacagttt atccaggggt ttgcggtggc ctgcttcttt atgccgctga ccaccattac
    14701 gctgtctggt ttgccaccgg aacgactggc ggcggcatcg agcctctcca actttacgcg
    14761 tacgctggcg gggtctatcg gcacgtcgat aaccacgacc atgtggacca accgcgagtc
    14821 gatgcaccat gcgcagttaa ccgagtcggt aaacccgttt aacccaaacg cccaggcgat
    14881 gtacagtcaa ctggaagggc ttgggatgac acaacagcag gcatcaggct ggattgccca
    14941 gcagatcacc aatcaggggc tgattatttc cgcaaatgag atcttctgga tgtcagccgg
    15001 gatattcctc gtcttgctgg ggctggtgtg gtttgctaaa ccgccctttg gcgcaggtgg
    15061 cggcggaggc ggtgcgcact aagtacaact aagccagttc atttgaactg gcttttttca
    15121 attaattgtg aagatagttt actgactaaa tgtgcagttc ctgcaacttc tctttcggca
    15181 gcgccagttc ttcgttgctg ttgatgcgca cgtcacgttc cagaatgcta cgcgcaatat
    15241 cctgcgcttc ctgcaacgag tgcatctggt aagtgccaca ctggtagacg ttcagttccg
    15301 ggatctgatt ctgatcctgc actttcagca cgtcttccat tgccgctttc caggcatcag
    15361 caacacgctg ctcatctggc gtaccaatca gactcatata aaaaccggtg cggcagccca
    15421 ttggcgagat atcgataatc tctacaccat taccgttaag atggttacgc ataaaaccag
    15481 caaacaggtg ctccagggta tggatccctc tttctggcat cacttctttg ttcggcacgc
    15541 agaagcgcag atcgaacacg gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca
    15601 cccgaactgc aggcgcttcc atccgggtat gatcgactgt gaagctatct aacaacggca
    15661 tttagccacc tccggtaatt tttttaaaaa ttttctgaac tctttcttcc caggcgagtc
    15721 tgagtatatg aaagacgcgc atttgttatc atcatccctg aattcagaga tgaaattttg
    15781 gccactcacg agtggccttt ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa
    15841 cggttcggta tctgcggctt ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc
    15901 tacaaaatcc tcttcgcgca gaatttccag cggctcttca cgcagcagat tacggtacgc
    15961 ttcagcaaac gctttgcctg ttccgccaat accagtatca atcatagacc ttaagatacg
    16021 ggcagagaaa gtcagatcgg gattatcgaa gcaggcgacc agttcatcac acactttctg
    16081 atacgcttcg ccgccgttaa tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa
    16141 cagatcttta cccacctgcg gtaacgggaa ctgtgcggtt tcgcagccga tacccagcgt
    16201 cagacccggt ttgcgacctt cgaggatcac ccggttccag ttaacgcgtg tacaggcaag
    16261 ttcgctactg ctcatttccg gtgcatcagc cagcgcacac cagaccataa acaggtcgag
    16321 gaatcgcacc tgctgttcat ctacaccaat cggcgagaac gggttgatgt ccagcgaacg
    16381 cacttcaata tattcaatgc cgccacgtaa cagcgcatca gaaggcgact cgccgctgcg
    16441 ggtaacgcgt tttggacgaa tcggcgcgta cagttcgttt tcaatctgca gcacgttgct
    16501 gttgatttgc agcctcttac cgtctttctc aataccaatc ttcgcgtact cttccgatgg
    16561 cgttttgatt gcctgtttaa ggcccgctac gtactcgtaa agatcgttga aggtaatacc
    16621 aagattgctt tgcgatttat tggtatagcc gagatcgctc aaacgaagag aggtcgcata
    16681 cggcaggtaa tacataccgc actcggtttt ctcaaacggc agcgacgttg gttttccttg
    16741 caggaaagaa gaacaaatcg ccggagatgc accaaacaga taaggaatga cccaaccgaa
    16801 acgatagtaa ttgcggataa cgcggaaata gcccgcagaa attttctctt tggcatcagc
    16861 gcccgagata tcaccgcact tcgcttgcca gaatgccatt ggcaaagaga aattgtaatg
    16921 cacgccggaa atggtttgca tcagcgcgcc gtagcgattt ttcagccctt cacgatacag
    16981 cgttttaaag cgtccggtgt tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc
    17041 tgcgatgtag catggcatac ttaacggcca catccgctca tcgcccatat tgcgcgccgt
    17101 ataacgatgc agatcgcgca taaaggtcag catatgttca atatcaccat ccactggtgt
    17161 aatgaattcc agcaatgctt ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga
    17221 acctaatgct tcaggatgac ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt
    17281 ttcgcgctcc agcccacgct gtatcccctt taacgcctga ggatgttttt ccagccaggc
    17341 cagcgcctgt gatacgtccg ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt
    17401 agcataactg taatggtgac catatgtgca ggcctacaat tagtgccacc acatcatgcc
    17461 ctgaacggtc gctgctgcaa ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa
    17521 gattaccggc ccccaggaga tgcgcatcca tcccgctaac agacacagta aatcgccaac
    17581 cacgggcatc cagcttaata atagcgtgac tgcaccatag cgtttcagcc agccggtagc
    17641 tttctcttgc cagcgcgatg ttttacgcaa tggaaagaaa cgcccaagga taacgttagt
    17701 taaccctcca aggctattac ccattgttgc tgttaaaact aaaacccagg gatgactgat
    17761 cccggaaagc aacattgcca ccagcacgac ttcggagttg ccgggtaata gtgtagcgct
    17821 gagaaaacta ctggcaaaca acgagaaaag cgataacgct tcactcacag caagcgaaca
    17881 tccacggcgt ccatgcctgc tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag
    17941 accacacact gcgtcggttg cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc
    18001 gcgggtttat ggtgtttgac gtgatcggca gcgacgacgg cgtcaaaata acggcgtaat
    18061 cccaggtgag ccagcaatgc ctcagcgatg gcgctttcac tccccgttcc taccgccatt
    18121 ggccgacgtc catgccaact tttcaccact tcaacaagag gaagcggctc gacactatcc
    18181 agcagcatac ttcttactgc ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg
    18241 gcctgattca gctcaataat tgcctgagca atacgccagg tgggcgatcc attaagcgca
    18301 atcatcgcct gaacatcgta ctgaagaccg tagtgcccta atacttcgcg ccacgcttta
    18361 cggtgcgtag gctccgtatc caggattgtg ccatccatat caaaaattaa acctgcataa
    18421 cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc gtaattgctt
    18481 gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag tagttattta
    18541 gaaatgagat atttttgaag gaaatttttt ggagaagatg gtgcatccgg gaggatgact
    18601 cactggcgtt cgcccttcgg gccgttgctg gcgcaacgtt atcatccctg gtgcctgcgg
    18661 ttaattccgc cctcttcgat accttcattg ctgaagattt cggagaatat ggtgcatccg
    18721 ggaggattcg aacctccgac cgctcggttc gtagccgagt actcta
//
LOCUS       DAPGRN010000095        18626 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPGRN010000095 DAPGRN010000000
VERSION     DAPGRN010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.095"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(276..4178)
                     /gene="hrpA"
                     /locus_tag="RHW33_003460"
     CDS             complement(276..4178)
                     /gene="hrpA"
                     /locus_tag="RHW33_003460"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV1646932.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFASLHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4379..4984
                     /gene="azoR"
                     /locus_tag="RHW33_003461"
     CDS             4379..4984
                     /gene="azoR"
                     /locus_tag="RHW33_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV1646933.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQLEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIIAA"
     gene            complement(5038..6354)
                     /locus_tag="RHW33_003462"
     CDS             complement(5038..6354)
                     /locus_tag="RHW33_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV1646934.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRETKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKSMLRLWENR"
     gene            complement(6344..8101)
                     /locus_tag="RHW33_003463"
     CDS             complement(6344..8101)
                     /locus_tag="RHW33_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV1646935.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVRDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLLLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVGDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRSRSPLKELHLLPGFYHDTLGEDNRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDSKPWVMRVRSQGEMDSLVRNAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8117..9013)
                     /locus_tag="RHW33_003464"
     CDS             complement(8117..9013)
                     /locus_tag="RHW33_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV1646936.1"
                     /translation="MLEKSLATLFALLILATLINRFRVWRLPERKGDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLAGFLILTTWRVLVGDTSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNTQGGALLVLFFLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAVKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(9013..9618)
                     /locus_tag="RHW33_003465"
     CDS             complement(9013..9618)
                     /locus_tag="RHW33_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV1646937.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLITGL
                     LLMLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFGTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLIWTAINRCRSVLLMSAER"
     gene            complement(9788..12094)
                     /locus_tag="RHW33_003466"
     CDS             complement(9788..12094)
                     /locus_tag="RHW33_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDV1646938.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDGQTIASMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSHLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNDVVTSLALKLAEMKHHGQLLRM
                     TPQESDKIAVYLYQKFENDDDLIGALFLALPDNLQFNFVKRMEKKSPAYFCCRDMQII
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETS
                     ADMILSELISYGEVDDQMLLNATSLIRSDDWDFLERALISWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVNEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(12158..13018)
                     /gene="pdxI"
                     /locus_tag="RHW33_003467"
     CDS             complement(12158..13018)
                     /gene="pdxI"
                     /locus_tag="RHW33_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV1646939.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKITEIVCVQNEYNIAHRADDAMIDALAREGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(13238..>18626)
                     /locus_tag="RHW33_003468"
     CDS             complement(13238..>18626)
                     /locus_tag="RHW33_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDV1646940.1"
                     /translation="VPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPPDDGGDTPVPDD
                     GGDTPVPPDDGGDTPVKHDPVVYKNDVTWDQDAKTVKIRETTFTYSQNADGSYTLTAP
                     EGKETIVKSWTVHDDSNTVIFDGVNTAGGITWSYDDDGLIHITKEAGVVVDGTTGTAI
                     DFGNAIITDQGGNTALNGGTVMTVEGDNITLNNDGKTTAIGEGSVVGILTGDDITINN
                     NGETEVDGGTAVIINGDNATLNTAGDSTITNGGTGSLINGDNARVDNQGTMSVDGENS
                     TGSKIVGDGATIKQEGDLYVSGGAHGIDVDGNDTFVSNKGNITVIEDNSIGMLLDGDG
                     VSVINMGDLNVGQAAAGENAIGIQIDGDNATFVNVGDISATNAGTGVSVAGDKANISL
                     AGGLDVGDFSTGLDVSGNNNNMTLATYELNVTGQKATGVNVSGDGNTIEIAGSILVDK
                     DQKADNAQPYFFNPSTGVNISGNNNDVTLDGQLTVVADSETTTRSYASYDGAQEHIAG
                     IVIAGDDNTFTLNGGVHFVGEKNVMDDGSKPAASRRGIGDTPLISVDGHSSVYLNGES
                     TISGEFPLGFENLIQLSNGAELEIGADATFDMSDVDSFTYYYRVALSTISIDSGAKAT
                     NNGEVELKNIGFAAAWNKDSTVINNGSIGLAMYDFGTDPAPKVFDVEYGGIGVNNGTL
                     TTKMMNQHSVLNYPAEWNLSDGTSFNNKALGLTGMLGSYSSSILNGETGIIDMYGRGS
                     VGMLVIDKSTADNEGQITLDTLWVDENDETSLRNNIANSTAKDFGVGMASGTDAYNGA
                     LTKATATNHETGVITVYNAGAGMAAYGNTNTVINQGTINLEKNENYNDSLGVNKLVGM
                     AVYHEGTAINDQTGVININAENGQAFYNDGTGLIINYGTICTFGVCQGADEYNPTDNY
                     IADLYDDGDVLSSAGETVLLSSGAIINGDVTSAGTVKNGTIRILENGTLVNEESGAIN
                     SAIVVNAGGALTNEGVLNTVTLEGGTFNNNGTLNDVVKIEKNSNAVINNTGSLSTLQL
                     NDGTVNNSGIASARVNAQGDAVFNNLAGGEARKGAILYNSAVVNNAGTWKMGYQDENN
                     NAGTLDIDDQSTFNNSGKLILDNSKNAIRFQGSNANATLYNTGEITLDAALGAGAIHY
                     DDGASQFINKGVVDAKVTVAVSTAGATASDAFLWNQDGGVINFDKDNASAVKFTHNNY
                     VALNDGVMNISGNNAVAMEGDKNAQLVNNGVINLGTEGTTDTGLIGMQLDANATADAV
                     IENNGTINIYANDSFAFSVLGTQGHIVNNGTVVIADGVTGSGLIKQGDSVNVEGVNGN
                     NGNNTEVHYTDYTLPDMPSSYTTSPFSETTDSGSSDGSSNNLNGYIVGTNVDGSAGKL
                     KVNNASMNGVGINTGFAAGTADTTVSFDNVVEGINLTDADAITSTSVVWTAKGSTDAS
                     GNVDVIMSKNAYTDVATDASVNDVAKALDAGYTNNELYTSLNVGTTAELNSALKQVSG
                     SQATTVFREARVLSNRFSMLADAAPKVGNGLAFNVVAKGDPRAELGNNTEYDMLALRK
                     TIDLSESQTMSLEYGIARLDGDGAQKAGDNGVTGGYSQFFGLKHQMSFDNGMNWNNAL
                     RYDVHNLDSSRSIAFSNTNKTADTDVKQQYLEFRSEGAKTFEPSEGLKVTPYAGVKLR
                     HTLEGGYQERNAGDFNLNMNSGSETAVDSIVGLKLDYAGKDGWSASATLEGGPNLSYA
                     KSQRTASLAGAGSQHFNVDDGQKGGGINSLASVGVKYSSKESSLNLDAYHWKEDGISD
                     KGVMLNFKKTF"
BASE COUNT         4701 a         5011 c         4224 g         4690 t
ORIGIN      
        1 aaatatacag cgcgcgtcgg taattttcct ctggaagttt ttcatagaaa gatattaaag
       61 cctgaccaac gcgatcaccc tctattatcc gttcaaattg cgacctcagt aaaatatcat
      121 gtctactcat tgcaatacgt cctgttcgcc cagggcgaag tatgaatgaa acttatgtaa
      181 aatagtaccg cccttgctat atttcagaat tcataaagaa aaaacccggc gctaaatgct
      241 taccgggttt tagatttatc aggcaaatag cagggttaac cgctaatctg ctccatcgcc
      301 tgcaaaatac gcttatctga aatcggataa ggcgtaccaa gttgttgagc gaagtaacta
      361 acgcgcagct cttctatcat ccaacggatc tctttcacgt cttcatcctc acgacgtgca
      421 ggcggcagtt tgttgatcca ttgctgccac gcctgctgga cgttttcgac tttcagcatc
      481 tgtgcacgat cgcgatgcgg atcaaccgcc agtttttcca gccgtttttc aatcgcctgc
      541 aaatatcgca gcgtgtcgcc cagccgtttg aagccgttac cagtgacaaa accgcgatat
      601 accagcccgc ccatctgcgc tttaatgtca gaaagcccca gcgccatggt catatccacc
      661 cgccctttca gacgtttgtt gatattgaag actgccgtaa ggatttgctc gacctgcttc
      721 gcaatatcca ccaccgtgtc gttcagttca gcgcgcactt tttcatgcag cgaagcaaag
      781 ccttcttccg tccagaccgg gccaccattg gcgtcgatca atttatccac accgcaggag
      841 atacagtcgt cgatcagctc cagcactttg ccatacgggt taaagtacag tccaagcttg
      901 gctttgtttg gtaacttctc atgtaaatac ttgattggtg agggaatatt cagcagcagt
      961 agacggcgaa gaccgttcca cattgcctgt ttttgctcca gcgggttatc aaacagtttg
     1021 atcgccacac tgtcgcgctc atccaccagc gccggccacg ccttcacttt gtagttgccg
     1081 cgcttctgct cgtagctttc cggcaactga ccaaaactcc agatatgtaa gccgctctgc
     1141 tcgataccgt catccgccac cgcagacagc gtttcctgca ctttgccttt cagcgcatct
     1201 ttcagatctt gtagcgagcg cccttctttt agcttcttgt ttttgtcatc caccacgcgg
     1261 aaggtgattt tcaggtgatc gggcacctga tcccagtgcc agtcttcgcg gtcaacggta
     1321 acgccggtca tccgccgtaa ctcgcgctca aggctgtcga gcaacggtaa ctccagcggt
     1381 ttgacgcggc ctaaaaacgc ttcggcatag tttggcgcag gtacaaaatt acggcgtacc
     1441 ggtttcggca acgatttaat cagagcaatc accagttcgc ggcgtagacc ggggatctgc
     1501 cactcaaacc cactctcctc aacctggtta agtaacggca gcgggatatg cacagtcaca
     1561 ccgtcagcat ccgcgccggg ctcaaactga tagctcaaac gcagcttgag attgccctga
     1621 tgccagaagt tcgggtaatc cagcttgctg atcttttccg ccccttcttt gatcaacatg
     1681 ctcttttcaa agttgagcaa atcaggggtt tcgcggctga cttttttcca ccagctgtcg
     1741 aagtgacgag cggagattac atcatggctg atgcgctggt cgtagaactc aaacagcgtt
     1801 tcgtcatcaa ccagaatatc gcggcgacgt gatttgtgtt ccagctcttc cacttcggcc
     1861 cgcagtttca ggttttcacg gaagaatgcg tgacgcgtct gccagtcacc ttccaccagc
     1921 gcgtggcgaa taaagagttc acgacataac gccggatcga tctggctgta gttgaccttg
     1981 cgcgcggcaa caatcggcaa accataaacg gtgacttttt ccgttgccat caccgcgccc
     2041 tgcgcccgtt cccagtgcgg ttcgctgtag gtacgtttaa tcaaatgctg agcgacgggt
     2101 tccacccatt ctggatcgat gcgtgccgca atacgtcccc acaggcggct ggtttctacc
     2161 agttccgcca ccattaccca tttcggcggt tttttgaata aaccagaacc ggggaagatg
     2221 gagaaacgcg cgttacgtgc gccggtatat tcttgtttat cggcatcttt catgccgata
     2281 tgggaaagca accccgtcag caacgcaatg tgaatttcac gatactccgc cggttcgctg
     2341 ttcaccggaa tgccgagttc tttcaccacc tggcgcaact gggtgtagat atcctgccat
     2401 tcgcgcacac gcagatagtt gagataatcg gtacgacaca ggcgacggaa ggcgttggaa
     2461 gaaagcgcct tttgctgctc gccaagataa ttccacagat ttacaaacgc gaggaagtcg
     2521 gattctttgt cgtggaagcg gcgatgtttt tcgtccgatg cctgctgttt gtccatcgga
     2581 cgttcgcgcg gatcctgaat ggagagcgca gacgtgataa tcatcgcctc acgcacgcag
     2641 ccatgttttt gcgcttccag caccatacgc gccagacgtg ggtcgacagg caactgcgag
     2701 agctggcgac cgagcggcgt cagtttatag gcgctggcct gttcatccgt ggtgatcgcg
     2761 cccagttctt cgagcagacg cacgccatcc tggatattgc gtttatccgg tgcttcgaca
     2821 aacgggaacg cagcgatatc gcccagcccc agcgcggtca tctgcaaaat aaccgaggcc
     2881 aggttggtac gcagaatctc cggatcggta aactccgggc gcgagaggaa atcgtcttcg
     2941 gaatagagac gaatacagat cccttcggac acacgaccac agcggccttt acgctgattg
     3001 gcagacgcct gcgaaatggg ctcaatcggc aaacgctgca ctttggtgcg atagctgtag
     3061 cggctgatac gcgccgtacc ggggtcgata acgtatttaa tccccggtac agtcagcgag
     3121 gtttccgcga cgttggtcgc cagcacaatg cgtcgcccgc tgtgcgactg gaacacgcgg
     3181 ttctgttcgc tattcgaaag ccgcgcataa agcggcaaga tttcggtatg gcgtaagttc
     3241 agcttgttca gcgcatcggc ggtatcgcga atttcccgct cgccgctcat aaagatcaga
     3301 atgtcgccgg ggctttcctg gcttagttcg tctacggcgt caaaaatcgc ctgcaactga
     3361 tcgcgctcgg tgtcatcggc ttcttcaaca atcgggcgat agcgcacttc caccggatag
     3421 gtccgaccgg agacttcaat aatcggtgca ttattaaagt ggcgcgaaaa gcgttccggg
     3481 tcgatagtcg cggaagtgat aatgattttt aggtcaggac gccgcggcag caactctttc
     3541 aaatagccga gcagaaaatc gatattcagg ctgcgttcgt gcgcttcgtc aataatgatg
     3601 gtgtcgtact gcatcagcag gcggtcttgc tggatctccg ccagcaggat accgtcggtc
     3661 atcagcttga ccatcgtgtt atcacttacg tgatcgctga aacgcacttt gtaaccgatg
     3721 caaccgcccg gctccgtttt cagctcttcc gcaatacggt tcgctaccgt tcttgccgcc
     3781 aggcgacgcg gctgggtatg gccgatcagc cctttaatcc cgcgccccag ctccatacag
     3841 attttcggta actgagtcgt tttaccagaa cccgtttccc cggcgacgat caccacctgg
     3901 tgatcacgaa tcgcttcgag aatgtcctgt tttttctgac taaccggtaa attgtcagga
     3961 taagtaattt ccggtcgtgc cgcttcacgc agcaggactt tccctgccgc ctggtcaatc
     4021 tctttcgcca tctcctggaa aatggcctgt tgtgcatcag gatttttaac cttcttcacg
     4081 ccgtgcagac ggcgagaaaa acgcagcctg tcacgcagca tcagcgaatc cagccgctgc
     4141 tgcaaggcag taaaggtcaa tttttgttgt tctgtcataa cgttagtggg cagtagtctg
     4201 cctgattaat tttcttttaa atgatagata tagagtacca catcgcggct ttctatgcgt
     4261 tgttcaataa attcgaacat aggcttcgat ttattgcgct atctctgtgg caggattgtg
     4321 aataaagtgt caacaagcaa cggggcatcg cccattcaaa catctataag gaaacaccat
     4381 gagcaaggta ttagttctta aatccagcat cctggcaggg tactctcagt ctaatcagtt
     4441 gtccgattat ttcgttgaac aatggcgcga aaagcactcc gctgatgaaa tcaccgttcg
     4501 cgacctggct gcaaatccga ttccggtact ggatggcgaa ctggttggtg cactgcgtcc
     4561 gagcgatgcg ccgctgactc cgcgtcagct ggaagctctg gcgctttctg atgagcttat
     4621 tgccgagctg aaagcccacg acgttatcgt tattgcggca ccgatgtata acttcaacat
     4681 atcgactcag ttgaaaaact attttgacct ggttgcacgc gcaggcgtta ctttccgcta
     4741 taccgagaat ggtccggaag gtctggtaac gggtaaaaaa gccatcgtta ttaccagccg
     4801 cggtgggatc cacaaagatg gaccaacgga cctggtgacg ccgtatctgt ccacgttcct
     4861 cggctttatc ggcattaccg atgtgaaatt tgtcttcgcc gaagggatcg catacggtcc
     4921 ggaaatggca gcgaaagcac agtctgacgc aaaagcagcc atcgacagca ttattgctgc
     4981 ataaccagac acggtcttga tgtggactgt agatattcag tccacatctc aatccattta
     5041 cctgttttcc cacaatctca gcatcgattt gtgttcgtct gtcagcacaa tctgcgggcg
     5101 tctggctcga atatagctaa tcgcttcatt aacggtttta cagtgtccgt aacataacaa
     5161 ccatgccgcc accaccagcg cactgcgcga taatcccaat gcacaatgga ccagaacgct
     5221 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagtt ccccctcttc
     5281 cggaaccacc aggtccaaca tcggtacaca gaaatagagt cgatcttttg tctctcgtcc
     5341 tcgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     5401 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     5461 acgcatagat agccacatcc cgatacgcca gggcaatgtc agccagtaaa cggcgggcgg
     5521 gagtttaccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     5581 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     5641 catcattagc tccatcaaca caatgcacgc gcccgcgcct acgacatatg gcagtgctat
     5701 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gtaccatcca
     5761 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccaggt
     5821 tgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     5881 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     5941 agactggtta tagggcagat caaacaattc aagttgcgaa aatagccatc cagtcacccc
     6001 actcacttca ggacggataa aactaaactt cagcgggtag agaaaaaaac cgcagcaggc
     6061 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgagctaca
     6121 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccatgg
     6181 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg gggatatcat ggctgttaag
     6241 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6301 caacaataac cagccagctc cctggagcaa tacgttccgg cgttcagtta tcacgacgca
     6361 ccgccatcga aaccgtaaaa ataccccatt catcaatccg ttgtgtgcat ttatcaaatc
     6421 cggcattacg cacgagtgaa tccatctccc cttgcgaacg cacgcgcatc acccacggtt
     6481 tactatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     6541 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagggagt
     6601 tttttacctg ctcattttcg ggaaaaagct catagaggcc agagacaatc gccagcgtag
     6661 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     6721 cccgcccaga cattccccgt tgagcaatca tctcttgccc ctgtgcaaca tttaactcac
     6781 tgtaatcacg taacaaaata tcacttacgg caggctcgtt cgccagcgca tccagtacat
     6841 agcgtccatg ccctgcggca atgtcaacca cgcggacggc taaccctttg gcgtggagat
     6901 cggcaacggc ttgtttaatc agcatttgta aatgggtttt gcgctggcga ataccgcgcc
     6961 agccaacact gttcaggtag attttgtcga ctaaacgccc gaatgcgtta ctaccttgcg
     7021 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccggtg ctcattccga
     7081 gatgcaggcc cgcagagtgc gccccgaaca atttcatcgc cttacgcata aagcgatacg
     7141 ccaaatcaac cggcgataat ggcacgggtc caccagaaag gagccgccat cgatccgctg
     7201 atggtccagt gcagtcttca tgctgataat caaatttttg cgatttgtta gcatataaac
     7261 ggctgataaa gctttgcatt ttttcaaatg cctgcgccct gttatcttca cccaacgtgt
     7321 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtgat ctctgataaa
     7381 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     7441 ggagcgtaat cgccgccgca tcgccaacaa tacgttcaga cgttttataa agatcgagca
     7501 agatattaac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     7561 gccgatcgtg ggtcaaatag cgtcctttca cataggaatt aataaaaaac aggccacgca
     7621 gacgatgcca taacgccagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     7681 ccggagaagc cagcagcagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     7741 ccagtactgc gccgacgctc tgcgcgatca ctaccacctc ttccagcccg acctggctgt
     7801 cgctggcagc aaaacggaca aactcatcga catcccgcac tgaacgcgca agagatgggc
     7861 tataaccacg gggccccgaa gtttgtccat gccctcgggc atcccatgca taaaaagcag
     7921 tatcaggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatgaccgc
     7981 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8041 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agggatgcgt gaattttcca
     8101 ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat accggagcgg
     8161 tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctggtaat agttttccgc
     8221 tatctttaac acccacatct cgtttgacgg ctgacatcac tacatcgccg caaaaaccac
     8281 taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt gtcagcaacg
     8341 gtccaataat cagtgacgca atcatggtgg tgatgacgcc ccccaccaga ccttccagcg
     8401 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     8461 actgtgcaat atcgtttgac tctgttaacg ccaaaaaaaa taacaccagt aacgccccgc
     8521 cctgggtatt tgtggtcggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     8581 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gtatcaccta
     8641 ctaaaactcg ccaggtggtt aatatcagaa acccagccaa aggtataaat aacagaaaca
     8701 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccaac
     8761 gcggaaaatg tacggatata agcgtacaat attctttcaa tgccagaaaa cttatcagcg
     8821 caaaaaacgt caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     8881 taccccacca ggtacgaata cgtaatgtaa cttcatcccc ttttctctcc ggtaaccgcc
     8941 atacccgaaa acggtttatc agcgttgcta aaattaacaa cgcgaaaagt gttgccagag
     9001 atttttccag cattatcttt cagcactcat caggagcacg ctccgacaac gattaatcgc
     9061 agtccagata agcagaattg aagcaatgct ccataaaaga ttattccatt gcatccactg
     9121 tggataaatg gcaacggcca acccccacaa cccaaatatt aacgcacgat cgcttttgcc
     9181 gaatggaccg gcataactgc gaataccgtt aatcgtctgc gcgagtaaac cgcaaaactc
     9241 agtcaatatg gtgccaaata acatgagtat tacgagtgat gcgttacttt ccggtaaaaa
     9301 taaaaacggt aaatagagcg caatatcgga aataacatcg ccagtttcat tcaaaatcgc
     9361 ccccagacgt gtttgctgat tgcactcacg cgccaacatg ccatccagcg cattgagtgc
     9421 catacggata aaaagcacga tgggcaatag caaaaagagg atgggttgtg cgaccaacat
     9481 caacagcaat ccggtaataa gagaaagcgc cagtgcagca agagtgatgt gattcgctgt
     9541 aacatggtgc ttataaagcc aaaacatcgt cggccttaag agcgactgaa agagcggttt
     9601 tatctggtag agcgtcatta tcaatccttg ataattcggt ttgcgctatg gtgcatcaat
     9661 tcccctgacg gagatatcgc aacacgacgc ttcattaaaa attcagaata tatcaaggta
     9721 ttggggactc actgtcagga atgattatcc caataaatca agtggttaaa aattattacg
     9781 ctgttattca atacaactca tcaagttttc tcatcgcttg ttcccgtatt gcgcgcgtct
     9841 taccgttaag cgccagctgg cgaactaaat ccagttcagg gttaacaagc tctaacgcca
     9901 gcaaaggatc acgtttcagc ctcgccttta atacatcaac tggaaaacgg ggattattca
     9961 tcgccacaat ccaccactcg ggatcaattt ctgccgccag tactgcaggc ggtgtgtggc
    10021 ggttgttaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt gtactccacc
    10081 atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg gggtctagtg
    10141 cataataaac acgtaacgct tcgttaacct gcgcacggga actgttttcc tgcctcagaa
    10201 aaaacttcgc ccaaatatca ttgcgtggcg tgttttgctg taattccttg agaacaacag
    10261 cgggtaaatt atcccaacta attaatgctc tctctaaaaa gtcccagtcg tccgaacgta
    10321 tcagagaggt ggcatttaat agcatctggt catcaacttc accgtagctg ataagctcag
    10381 acaaaatcat atcagcgctg gtttcatatg catctgaatc tgctttggga ttatttttcc
    10441 gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga tactgatttt
    10501 tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt tgtaaagcag
    10561 cgtcagagtg aattatctgc atatcccgac agcaaaagta ggcaggggat tttttctcca
    10621 tccttttaac aaaattaaac tgtaagttat ctggcaacgc taaaaataac gcacctatta
    10681 gatcatcgtc attctcgaat ttttggtata aatatactgc tattttgtca ctctcttgtg
    10741 gcgtcatccg caacaattga ccatggtgtt tcatctctgc cagctttaac gctaagcttg
    10801 tgacaacatc gtttgatgaa tcgttcgcca actgcattgc ctgattgtag gtgagttgtc
    10861 ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca tcaataatgg
    10921 attcgggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc caatctcttt
    10981 tcgccagagt catgacgata tccagaggga gattttcacg tagcgcaaga ttcaacggac
    11041 cgacaccatc aggtagatga ctcattcgcg tcattatttc gacagagagc ggttcacggt
    11101 cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac tccagaatct
    11161 cattgtcagg catatatctc gccatacttg caatcgtttg tccatcattg ctattaataa
    11221 gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgc attatggcaa
    11281 gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca tccagcattt
    11341 gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct tttttcatct
    11401 gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct ttcagagcat
    11461 cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc agcgcttttt
    11521 tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta tttgtcgaag
    11581 acgcaacaac aggtgcaatt atgtcattga taaatatccc gcgcgcatta atactcccct
    11641 taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt gcatttatcg
    11701 aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgtcacg ttaccgagaa
    11761 taacccatgg attagaaaga gagaaagtgg aaatattacc tttgactgtc aaattgccaa
    11821 gaacaatcaa tttttgatta ctctctatat taccttctac cactaaatcg ccacgatgca
    11881 ctttccagtc cgatgcagat aaaccgaagg ggacaagatt cacgctactg ttgatagctt
    11941 gttcaacttg ttgtttatct tcagcggtag ccgcagaaaa aaaaggttca atatcaactg
    12001 catttgcaac gcatcgaagt tccgcctgta ctggaagact cagcataaac aggggaagta
    12061 aacctatcca gctgcaaatc ctgggcttaa acatatgcac tccatgccat taaaaaagaa
    12121 acaaaaggga aatgcatctt tcccttttgt atattcgtta ttctcgcgaa ataccatcca
    12181 acgtagacaa cacttcctca gaaagatgca atttttcagc agccatattc tcccgtaaat
    12241 gcgctacaga agacgtccct gggatcagca aaatattcgg tgaacgctgt aacagccacg
    12301 ccagcgccac ctgcattggt gttgcaccca ggctcgcagc aacatccgac agcgtagagg
    12361 attgcagcgg cgtaaagccc ccgagcggga agaacggcac ataggcaatg ccttcgcggg
    12421 ccaaagcatc aatcattgcg tcatcagcac gatgagcaat gttgtattcg ttttgcacac
    12481 agacgatttc ggtaatctta cgcgcctctg caacctgcgt cggtgtgacg ttgctcaggc
    12541 caatatgttt taccaggcct tgttgttgca tctctgccag cacggtcagg ctggcttcaa
    12601 tcgatccttc cgccgggcca tgaccatccc ccatcatcac gcgcaggtta accacatcca
    12661 gtacgtccag gccgagatta cgtagattat cgtgtaccgc tttttgcagc tctgccggag
    12721 aaaaggcggg caaccaggaa gcgtcttctc cacgccgcgc accaatttta gtgacaattg
    12781 tcaggtcgtc agagtaagga taaagcgctt cgcggataat ctgattggtg acgtgcggac
    12841 cataaaaatc gctggtatca atatgattga cgcccaatgc cagcgcctcg cgcagcacgg
    12901 taatagcgac atggcgatct ctgggggggc cgaaaacccc aggacctgcc agttgcatcg
    12961 cgccataacc aagacggtta acggattttg taccgagagt aaatgtattg ctgctcatcg
    13021 caccctgccc tgtaataaaa cggattaaga aaaatcagta tagaaacgaa aagagataaa
    13081 taatgcgaag gcgtaaacaa gatttctgga tgagtagcac tattgtcctg aaagacacca
    13141 aaacattaga cagcaaaaga taagtcaagc gtcgcatccg gcaataaaaa accgcaacct
    13201 gtatcagcgt tgcggtttgt gtatcacatg ctaaaaatta gaacgttttc ttaaagttca
    13261 gcatcacgcc tttgtcgctg atgccgtcct ctttccagtg atacgcatcc agattcagcg
    13321 aactttcttt gctactgtac ttcacgccga cgctcgccag gctgttgata ccgccgccct
    13381 tctgaccgtc atcgacgtta aagtgctggc tgcctgcccc tgcaaggctt gccgtgcgct
    13441 ggctcttcgc gtagctcagg ttcggcccgc cttccagcgt agcgctagcg ctccagccgt
    13501 ctttacctgc gtaatccagt ttcaacccga cgatgctgtc caccgccgtt tcgctgccac
    13561 tgttcatatt caggttaaag tctccggcat tgcgctcctg atagccacct tccagtgtgt
    13621 gacgcagttt tacacccgca tatggcgtaa ccttcagtcc ttcgctcggt tcgaaagtct
    13681 tcgccccttc gctgcggaac tccaggtact gctgcttcac gtcggtatca gccgttttgt
    13741 ttgtgttgct aaatgcaatc gaacgactac tgtcaaggtt gtgaacgtca taacgcaatg
    13801 cgttattcca gttcatgccg ttgtcgaagg acatctgatg tttcaggcca aagaactggc
    13861 tataaccacc tgtgacgcca ttatcacccg ctttctgcgc accatcacca tcgagacgag
    13921 cgataccgta ctccagactc atcgtctggc tttcgctcag gtcgatggtt ttacgcaatg
    13981 ccagcatgtc gtattcggta ttattaccta actcggcacg tggatcgcct ttcgcaacaa
    14041 cattgaacgc cagaccgtta cccaccttcg gcgcggcatc cgccagcatg ctgaagcggt
    14101 tgcttaacac acgcgcttca cggaataccg tggtcgcctg gctaccgctc acctgcttca
    14161 gggcgctatt cagttcagca gtggtgccca cgttcaggct ggtatacagc tcgttattgg
    14221 tgtaacccgc atccagtgcc ttcgccacat cgttcaccga agcatcggtc gccacatcgg
    14281 tgtaggcgtt tttgctcatg atgacgtcaa cgttgccgct ggcatcggtg ctgcctttgg
    14341 cggtccatac cacggacgtt gaggtgatgg catcggcgtc ggtcaggttg atgccttcca
    14401 ccacgttgtc gaaactgacc gtggtgtctg ccgtaccggc ggcgaaaccg gtgttgatcc
    14461 cgacaccatt catgctggcg ttgttgacct tcagtttacc ggcgctgcca tcaacgttgg
    14521 taccgacgat atagccgtta agattgttac tgctgccatc gctactaccg ctatcagtcg
    14581 tttcactgaa aggtgaagtg gtgtaagagc ttggcatgtc cggcaacgtg tagtcggtgt
    14641 aatgcacttc ggtattatta ccattgttgc cgttcacccc ttccacgttc acgctatcgc
    14701 cctgcttaat caacccggaa ccggtcacgc cgtcggcaat caccaccgta ccgttgttaa
    14761 cgatatggcc ttgtgttccc aacacactga atgcaaagga atcgttagca tagatattaa
    14821 tcgtgccgtt gttctcgatc accgcgtctg cggtagcgtt ggcatccagt tgcataccaa
    14881 ttaacccggt atcggtggta ccttcggtgc cgagattgat aacgccgttg ttaaccagct
    14941 gtgcgttttt atcaccttcc atcgccacgg cattgttgcc gctgatgttc attacgccat
    15001 cgttgagagc aacatagttg ttatgggtga atttgactgc gctggcattg tctttatcga
    15061 agttaattac cccgccatcc tggttccaca agaaggcatc gcttgctgtc gcaccagcag
    15121 tacttaccgc cacggtgact ttcgcatcca ccacgccctt gttaataaac tggctggcac
    15181 catcgtcgta gtgaatagcg cccgcgccaa gtgcggcatc cagtgttatt tcaccggtgt
    15241 tatataacgt ggcattggca ttgctaccct ggaagcggat ggcgtttttg ctgttatcaa
    15301 ggatgagctt gccgctgttg ttgaacgttg actgatcgtc aatatccagc gtcccggcat
    15361 tgttattttc atcctgataa cccattttcc aggtccccgc gttattcact accgcagagt
    15421 tatagaggat cgcgccttta cgcgcctcgc cgcctgcaag gttattgaat acagcatcgc
    15481 cctgagcgtt aacgcgcgcc gacgcaatac cgctgttatt caccgtacca tcgttaagct
    15541 gtaaggttga cagggaaccg gtgttattaa ttaccgcgtt gctgtttttc tcaattttga
    15601 cgacgtcatt aagcgtgccg ttgttattaa aggttccccc ttccagagtc accgtgttca
    15661 acacaccctc attggttaac gcaccgcctg cattcacgac gatggccgaa ttgattgctc
    15721 cgctttcctc gttaaccagc gtgccgtttt cgagtatcct gatcgttcca tttttaacgg
    15781 tacccgcact ggtgacatcc ccgttgataa tcgcacctga cgataataat acggtctcac
    15841 ccgcagagct taatacatcg ccatcatcat acaaatcggc gatataatta tccgtcggat
    15901 tatactcatc agccccctgg caaacaccaa aggtacaaat ggtgccatag ttgataataa
    15961 gtccagtgcc atcgttatag aatgcctgac cattttccgc gttgatgtta attacgcctg
    16021 tctggtcatt aatggcggtg ccttcgtgat aaaccgccat gccaacgagt ttattcacac
    16081 cgagggaatc gttgtaattt tcatttttct ccaggttgat cgtaccctgg ttaatgaccg
    16141 tattggtatt accatacgcc gccatccccg cacccgcgtt gtagacggta attacgcctg
    16201 tttcgtggtt ggtagccgtg gctttggtta atgccccatt ataagcatcg gtacccgagg
    16261 ccattcccac accgaaatct ttcgctgtgc tattggcgat attattacgc agcgatgtct
    16321 catcattttc atcgacccac agcgtatcca gggtgatttg cccttcgtta tccgcggttg
    16381 atttatcgat gactaacatc cccacgctac cacggccata catatcaata atgcccgttt
    16441 cgccgttcag aatggatgac gagtagctgc caagcatacc tgtcagccct aatgctttgt
    16501 tattgaaaga ggtaccatca gacaagttcc actctgccgg ataattcagc acactgtgct
    16561 gattcatcat tttggtggtc aaggtgccat tgttaacgcc aataccccca tattcaacgt
    16621 caaagacttt tggcgccggg tcggtaccaa aatcgtacat cgccaggcca atggatccgt
    16681 tattgatgac cgttgaatct ttattccagg cagccgcaaa accgatattt ttcagttcca
    16741 cttcaccgtt gtttgtcgcc ttcgcaccag aatcaatact aatggtggat aacgctacgc
    16801 gataataata agtgaaagaa tccacatcgc tcatatcaaa cgttgcgtca gcgccaatct
    16861 ccagctccgc gccattactc agttgaatga gattttcgaa acccagcggg aactcaccgc
    16921 tgatggttga ctcaccattg aggtagactg aggaatgccc atcaacgctg ataagcggtg
    16981 tatctccgat accacgacga gatgcggcag gttttgagcc atcatccatg acattttttt
    17041 caccgacaaa atggacaccg ccattaagcg tgaaggtatt gtcatcgcca gcaataacaa
    17101 tacccgcaat gtgctcctga gcaccgtcgt aacttgcata ggaacgcgta gtggtttcgc
    17161 tatcagcaac aacggttaac tgaccatcca gcgtgacgtc attgttatta ccgctgatat
    17221 ttacgccggt tgagggatta aagaagtaag gttgcgcatt atcggctttc tgatctttat
    17281 caaccagaat gctgcctgcg atttcaatcg tattgccatc acccgaaaca ttaacacccg
    17341 tcgctttttg ccctgtcaca ttcagctcat aagtcgccag cgtcatgttg ttattattgc
    17401 cagagacatc aagaccggtg gagaaatccc caacgtctaa cccacccgcg agtgaaatat
    17461 tagctttatc cccagcaacg ctgacaccgg ttccggcatt ggtcgcggaa atatcaccaa
    17521 cgttaacaaa tgtcgcatta tcaccgtcga tctgaatgcc aatggcattt tcacccgctg
    17581 cggcctgacc aacattgagg tcgcccatgt tgatcacgct gacgccatca ccatcaagca
    17641 gcatgccaat agagttatct tcgatgaccg taatattacc tttgttgcta acgaacgtat
    17701 cattgccgtc aacgtcaata ccgtgcgcac caccgcttac gtataagtcg ccttcctgtt
    17761 tgatggtggc accgtcaccg acaattttcg agccagtgga attctcaccg tcaacgctca
    17821 tggtaccttg attatcgacc cgcgcattat cgccattgat aaggctaccc gtaccaccat
    17881 tggtgatggt cgagtcgccc gcagtgttca gcgtagcatt atcgccattg atgattactg
    17941 cggtaccgcc gtcaacttcc gtctcgccgt tgttattgat agtgatatcg tcaccagtaa
    18001 ggataccaac gaccgaaccc tcgccgatgg cggtcgtttt accgtcgttg ttgagggtga
    18061 tgttgtcacc ctcaaccgtc atgacagtac cgccattcag cgccgtattg ccgccctgat
    18121 cggtgatgat cgcattacca aaatcaatcg cagttccggt tgtgccatca acaacgacac
    18181 cggcctcttt agtgatatgg atgagcccat catcgtcgta gctccaggtg atgccacctg
    18241 ccgtatttac gccatcaaag atgacggtgt tgctgtcgtc atggactgtc caggacttaa
    18301 ctatcgtctc cttgccctca ggcgcggtta aggtgtaact cccatcggca ttttggctgt
    18361 aggtaaaggt ggtttcgcga attttgacgg ttttcgcgtc ctgatcccag gttacgtcat
    18421 ttttatagac gacaggatcg tgtttaaccg gggtatcgcc accatcgtct ggcggaacag
    18481 gcgtatcacc gccgtcgtct ggaactggcg tatcaccgcc atcatctggt ggaaccggcg
    18541 tatcaccgcc gtcatctggc ggaaccggcg tatcaccgcc gtcatctggc ggaacaggcg
    18601 tatcaccgcc gtcatctggc ggaact
//
LOCUS       DAPGRN010000096        18501 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPGRN010000096 DAPGRN010000000
VERSION     DAPGRN010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.097"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            153..1373
                     /locus_tag="RHW33_003469"
     CDS             153..1373
                     /locus_tag="RHW33_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV1646941.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1346..1975
                     /locus_tag="RHW33_003470"
     CDS             1346..1975
                     /locus_tag="RHW33_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV1646942.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1976..3136)
                     /gene="ybdL"
                     /locus_tag="RHW33_003471"
     CDS             complement(1976..3136)
                     /gene="ybdL"
                     /locus_tag="RHW33_003471"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV1646943.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3245..4333
                     /gene="hcxA"
                     /locus_tag="RHW33_003472"
     CDS             3245..4333
                     /gene="hcxA"
                     /locus_tag="RHW33_003472"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV1646944.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4343..4540)
                     /locus_tag="RHW33_003473"
     CDS             complement(4343..4540)
                     /locus_tag="RHW33_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV1646945.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4722..6827)
                     /gene="cstA"
                     /locus_tag="RHW33_003474"
     CDS             complement(4722..6827)
                     /gene="cstA"
                     /locus_tag="RHW33_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV1646946.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7008..7421)
                     /gene="entH"
                     /locus_tag="RHW33_003475"
     CDS             complement(7008..7421)
                     /gene="entH"
                     /locus_tag="RHW33_003475"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV1646947.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7424..8170)
                     /gene="entA"
                     /locus_tag="RHW33_003476"
     CDS             complement(7424..8170)
                     /gene="entA"
                     /locus_tag="RHW33_003476"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV1646948.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8170..9027)
                     /gene="entB"
                     /locus_tag="RHW33_003477"
     CDS             complement(8170..9027)
                     /gene="entB"
                     /locus_tag="RHW33_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV1646949.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMAQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9041..10651)
                     /gene="entE"
                     /locus_tag="RHW33_003478"
     CDS             complement(9041..10651)
                     /gene="entE"
                     /locus_tag="RHW33_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV1646950.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVVPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLITKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPSEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10661..11836)
                     /gene="entC"
                     /locus_tag="RHW33_003479"
     CDS             complement(10661..11836)
                     /gene="entC"
                     /locus_tag="RHW33_003479"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV1646951.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQLLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12025..12993
                     /gene="fepB"
                     /locus_tag="RHW33_003480"
     CDS             12025..12993
                     /gene="fepB"
                     /locus_tag="RHW33_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV1646952.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQALLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLNSLFKKDL"
     gene            complement(13071..14321)
                     /gene="entS"
                     /locus_tag="RHW33_003481"
     CDS             complement(13071..14321)
                     /gene="entS"
                     /locus_tag="RHW33_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV1646953.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGFSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14432..15436
                     /gene="fepD"
                     /locus_tag="RHW33_003482"
     CDS             14432..15436
                     /gene="fepD"
                     /locus_tag="RHW33_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV1646954.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15433..16425
                     /gene="fepG"
                     /locus_tag="RHW33_003483"
     CDS             15433..16425
                     /gene="fepG"
                     /locus_tag="RHW33_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV1646955.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQIFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16422..17237
                     /gene="fepC"
                     /locus_tag="RHW33_003484"
     CDS             16422..17237
                     /gene="fepC"
                     /locus_tag="RHW33_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1646956.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17234..18367)
                     /gene="fepE"
                     /locus_tag="RHW33_003485"
     CDS             complement(17234..18367)
                     /gene="fepE"
                     /locus_tag="RHW33_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV1646957.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKMKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4377 a         5230 c         4760 g         4134 t
ORIGIN      
        1 ttttgatata taacatcaga cagctactat aactttcacc tatttattca cagcgcgaat
       61 gaaaaataaa acactctgct tttcagtgtt tactttataa ttaacagtct tatctcactg
      121 atcaccctgt aacgttcaga gagcgtcttc agatgtctat ttataaaatt cctcttccgc
      181 tcaatattct ggaagctgca cgagagcgta ttacctggac actcaacacc ctaccccgcg
      241 tttgtgtctc attttctggc ggcaaagact ctggtttaat gctgcatctg acagccgaac
      301 ttgcccgaca aatgggcaaa aaaatctgcg ttttgtttat cgactgggag gcgcaattct
      361 cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc atcgaagagt
      421 tttactgggt tgcgctcccg cttacgacgc aaaattccct ttcacaatac caacccgaat
      481 ggcagtgctg ggaacctgat gtcgaatggg tgcgtcagcc cccacaagat gcgataaccg
      541 atcctgactt tttctgcttt taccagccag gcatgacctt cgaacaattt gtacgtgagt
      601 ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc cgtgcggatg
      661 agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt gccgacgata
      721 aaccctggac tacggccgca ccaggcggtc atagctggta catttacccc atttacgact
      781 ggaaagtggc tgatatctgg acctggtatg caaatcatca aagtctctgc aacccactgt
      841 ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc gaaccttttg
      901 gcccggagca acgacaagga ttatggctct atcacgttat cgaaccggat cgctgggctg
      961 ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga catgacaatc
     1021 atttctatgg gcatcggaaa atcctcaagc cagaacattt agactggcaa gaatatgcat
     1081 tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat aaaatcgcca
     1141 tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag caaggggaca
     1201 ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc aataacgatt
     1261 actggtgccg ggcattatca tttagtccta cgaaagcgaa gaactatcag cgttataacg
     1321 aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat taacgcagga
     1381 tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaacgcga ttaatgaaat
     1441 ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt gcgttctgtg
     1501 ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg caccacccga
     1561 aaaaaaactg ctgaaaaaat ccattgaaat agatggtttt acccaaccca ttgtggtcac
     1621 gcatacggat aaaaatgccc tggaaatcgt ggatggtttt caccgccatg aaattggtaa
     1681 aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct gtctggaagg
     1741 tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg gacgccatca
     1801 aatcaccgcc atgtcggaaa tcgtccgtga actcagtcag ttgggatggg acgataataa
     1861 aattggcaaa gagctgggaa tggacagcga cgaagtgctg cgcctgaagc aaattaacgg
     1921 tctgcaagag ctgtttgccg accgtcaata ttcccgcgcc tggactgtca aataactaca
     1981 gctggcgcag tcgttcagct gctgccagca acgtcgattc cttcttggca aaacagagac
     2041 gaatcagttt atgtgggaag ggatcggcgc aaaacaccga tagcggaatc gccgccacgc
     2101 cgtgctcccg cgtcagccac tggcaaaact caacatcatc cagggtagaa accgcgctgt
     2161 aatccaccag caaaaagtat gtgccttcgc acggtaaaat ctccagccga ctttcattta
     2221 aggcatttac cagaatatcg cgcttctggc gataaaagtc cggtaacgca agataatgct
     2281 caggttctgc acgtagcata tccgcaattg ccagctgtgc cggggtattc accgaaaagg
     2341 tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca acacaataac
     2401 ccactttcca gccggtcata tgataggtct tgccaaacga tgacaccgcc actgcccgct
     2461 cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag ttgatgtgct
     2521 cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc cacaaagcgg
     2581 cgaaatcagc ctgctgccag acagttgcac tgggattatg cggagtgttg aggatcacca
     2641 gtctggtgcg ctcgcttaac aatgcggcaa attcctgcca gtcaacgcga aaatgcggtg
     2701 gttgcagtgc catacgcttc actattcccc cagaaagcgc gatagcgggg gcgtaactgt
     2761 catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg gtaatcgccg
     2821 cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca tctggttgat
     2881 agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc acgccggtca
     2941 tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc tgtaaatagc
     3001 gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt tgctgcgcca
     3061 gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt tgtggaatca
     3121 gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg cactataaca
     3181 cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat aaggacaaca
     3241 caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact tttcacatcc
     3301 aggaagtttc aatcacctgc acgatttttt cactgatgaa caactttctc gcgcggtgtg
     3361 gatctacggc gaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag cgtttggact
     3421 tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg tacagcaact
     3481 ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg gcgcactact
     3541 cgacaccgcg aaagccctcg cccgccgtct cggtctgcca tttgttgccg ctccaacgat
     3601 cgccgccacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg ccggacaggc
     3661 gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac cagagattat
     3721 cctcaatgcg ccgcaacaat atctgctggc ggggatcggt gatacgctgg cgaaatggta
     3781 tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc gactggggat
     3841 caataatgcg caggctattc gcgacgtctt gttaaacagt agcgaacagg cgctgagcga
     3901 tcagcaaaat caacagttaa cgcaatcatt ttgcgatgtg gtggatgcga ttatcgccgg
     3961 gggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg ctcatgccgt
     4021 gcataacggt ctgaccgtgc tgccgcaaac cgagaagttt cttcacggca ccaaagtcgc
     4081 ctacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgttctgg cgcaattaac
     4141 tggagcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag tggatatcaa
     4201 taatcaggcg gagatcgaca aagtgattgc ccacaccttg cgcccggtgg aatccattca
     4261 ttacctgccg gtcacgctga caccagatac gttgcgtgca gcgttcgaaa aagtggaatc
     4321 gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca ccgtagcgcg
     4381 cgtcctgccg ctcacggaaa aactcttcgt aggtcatcgg cgtttgatcg ggatggttaa
     4441 cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc ttcgccgcct
     4501 gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc gctaaccatg
     4561 ttgctgaaag agtgccggat gcggcgtgaa cgccttatcc ggcctacaaa tgctatcaac
     4621 gcgctcggtc aatcccctcg ccccatcggg gagagggtta gggtgagggg gaaccctcgg
     4681 cactatgcca acattcgcca acatcccccc tcactctgac tttagtgcgc gcctttcgcc
     4741 tgcgctacga tctcctcaac attttccggc atcggctcat acggcgtttc tttcgccgtt
     4801 ggcttcggat ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc cagaaccacc
     4861 acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa caccagttgc
     4921 gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat tgcctggaac
     4981 ttgttagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt ctgccagcca
     5041 gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca ggcgtaacgt
     5101 tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc tgccagcatc
     5161 tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg atcgaccacc
     5221 ccctgatgca ggaagtagcc ccacgccagc acgcacagcg ccgttgccag caggttagca
     5281 ggcagtgaat cggtccgttt caggcccgga gaaaccacgc ccagcagatc ctgcaacata
     5341 aagcgcgcag cacgcgtacc tgcatccacc gccgtcagaa taaacagagc ttcaaacaaa
     5401 atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc gtgcaggatg
     5461 taagccatcc ccaccgccag cgtcggcgca ccgcccgcac gggaaatgat cgactgttca
     5521 cccacttcgc tggcaatctg gttgagcgta tccggagtaa tcgcaaagcc ccagctactc
     5581 accacttgcg cggcagaagc gactacatcc gccgtccctg caggagccag cactgccatc
     5641 gggctgttca tggcgaagta cacacctgga tcgatgatac aggcggaaac cagcgccata
     5701 atcgctacga aggattccat caacatcccg ccgtagccga taaagcacgc ctgcccttcg
     5761 ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc agacaccgcg
     5821 ccacaggcaa tggtgataaa caggaacggg aacaggttac cggtccatac cgggccagtg
     5881 ccatcaacaa atttagtcaa cgcaggcatg gtcagcgtcg ggcgcataat caaaatgcct
     5941 accgccagac cgacgatagt gccgattttc aggaaggtag agaggtagtc acgcggggcc
     6001 agcagtaacc acaccggcag caccgccgcc acaaaaccgt agcccaccag catccaggtc
     6061 agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg ggctttctgc cacccagccg
     6121 ccagagataa tggcaaaaat caggaacacc aggccaatca ctgacacttc gccaatacgc
     6181 cccggacgca gatagcgcag gtagattccc ataaaaatcg ccagcggaat ggtgaacgca
     6241 acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat cgccagtact
     6301 gcaaggataa tgaccatgat cataaagcag gccaccagcg caatcacccc ggcagttggc
     6361 cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt agaaacaaac
     6421 agcaccatga aatcctgcac cgcaccggcg agaaccaccc cggcgagcag ccagatcatc
     6481 cccggcaggt aacccatttg cgccgccagt accggcccca ccagcggacc tgctccggca
     6541 atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata gtccagcccg
     6601 tcgttatggc gcaccgctgg cgtcatacgc gtcgggtcaa ctgccagcac atttttggcg
     6661 atatacagcc cataaaaacg gtaagcgatc agatagatac agaccgacgc caccacaatc
     6721 cacagtgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc aaatgctccc
     6781 attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt tgttatccgt
     6841 gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgcgca atttaacaac
     6901 acagcaacca tgcaaatcgg agttgaaacc aatatttaac ttaacaatgt taactcgatc
     6961 actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca tcccaaaact
     7021 gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac gacgatttcc
     7081 cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac cttgccttca
     7141 gacaccgggc gatgatgggt ggcgttaagt tccgtgccca ccacacactg cccgtcacgg
     7201 gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga cgcgccgcca
     7261 tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc ttccagcaca
     7321 tcatcgccca gacgggtata cacaattccc agatgcgcca ccattgtgtt atcgctggtg
     7381 gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc cccagcgttg
     7441 agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca gaggcgagga
     7501 acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga atgccgagtt
     7561 taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc acccacagcg
     7621 tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc acgccgctac
     7681 ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc gatgcgccat
     7741 aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgcaacagtg acaatcgccc
     7801 cgccccgctg acggcgaaac tggttcatgg tctgctgaaa caggttaaac gcaccgccga
     7861 cgttaaccgc aaaagtctgc tgccagtcct ctttgctcag ttggtctgtc gcgcccatgc
     7921 gtaaaattcc cgccgcattg accagcgcgt ccagtcgctc cgtttcagct aacagtcgct
     7981 gacacacttg cgcgacctgc gcagcgtcgg caacatccat cacttcggtc gcaaagggat
     8041 attgctcctg agtgaacgct tgatcaaaac ctgtaacttt cgctcccgct tcaacaaacg
     8101 ccagcgccgt ggcgtagcca atacctttcc ccgcgccagt tacccagaca tttttaccgc
     8161 tgaaatccat tatttcacct cgcgggagag tagcttccac caggcgtcga tggtcgggtt
     8221 tttcgccagc atgacaaagt cgatatcgcc atgcactttg cgccaacgcg ccgccagcgc
     8281 catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga acggttcatc
     8341 ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg cggggatagg
     8401 tgctggcagt aattcttcgg tcatcaccac ccggccagaa cgtccggcca catatttcag
     8461 cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca taaacggttt
     8521 aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg catatacccc
     8581 ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg gagaacgatg
     8641 aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg tcagtcgatc
     8701 caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat cattcaacag
     8761 cgccctatct tcatcgcttt gctctttcgg ctgggcggtg taataaaccg ggatattgtg
     8821 ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgcgcca tcatcgggca
     8881 gttctcaccc cagaagctga caaaatagtc ctgcatatca tggattaaca acgcagcacg
     8941 ttgcggttca aaggcccagt cgactttatt cttagggata tcgtgagact ccggcagtgc
     9001 gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgac gcgcgtgacg
     9061 ccagccactg acgtaattgt tttttatcga ctttcccgac cgccgtcagc ggaagtgaat
     9121 ccacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca cgcaggaaac
     9181 gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcacag cttttttcgc
     9241 ccatcagctc atcttccata ctgaccagtg cggcgtagat caccgccgga tggcgcagca
     9301 acaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc tgatctttct
     9361 cgcgcccctg cacggtgatg taaccctctg gatcaataga gatcagatcg ccggaacagt
     9421 aaaaaccgtt ggcatcaaag gcactggcat tgtgctgcgg acttttgtaa taaccgcgga
     9481 aggtatacgg cccgcgcgtc atcagtcgtc cgacttcccc ttgcggcagt ggatttcctt
     9541 cggcatcggc aacccatact tcgtcatccg gacacattgg gtaaccctgg gtatggataa
     9601 ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc atgccaaaca
     9661 cctgctgcaa ctggcagcca atctcagagg gaatacgggc cgcaagcgtg gcagaaagac
     9721 gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt tcgccttcgg
     9781 tcagcgcttg caaccacagg ctgactgccg gcggcaccag cgcggtaacg ttaacctgat
     9841 gttttgtaat caatggaaag cagagcgtgg cgctgggatc ggccgccaga acaaccgttc
     9901 ctccggcaag aaagacgccc agcgatcccg gcgaactcat ggcgtagtta tgagccgccg
     9961 ggatcgcgca caggtagcgc gtctgttgtg tgaactgaca aatctcgacg ctacgacgca
    10021 cgctgtagta gtagtcgtta tgagtgcgcg ggatcagttt cggtgtgccg gtggtgccgc
    10081 cggaaagctg gaaataggcc acttcatcag caggtgacgg cgtggcagta aaatcctcag
    10141 ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc agttgcacca
    10201 cgcgaatgga ggagtgctct gcaacaaatg tgttgaggaa atcatccccg ctaaatagcg
    10261 catgttggcg atcggcaatc agtaacgccg gttcaatctg gctggcatag gcgttcagtt
    10321 cactacgctg atggctgaac agcgccagca ccggcacgac gcccagtttc agcagcgcga
    10381 aaaaggtaat gtaaagttca gcgacgttac ccagttgtac cagcgcggtt tcaccgggtt
    10441 taatgccctg acgtcgcaag ctacacgcga gattatccgc cgcctgattc agctcccgat
    10501 aactcaactg ccgctcgccg tcgataaccg cgatgctgtc actcgcagcg tggcgagtca
    10561 gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga cgggcaaact
    10621 cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat ccaaaaacgt
    10681 tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt gacgacgcag
    10741 gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc gcgcagcgga
    10801 tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg ccgccaaaca
    10861 gttcgcggtc gaacggctcc agttcagcaa taacctgggt cgcggcctga tgcgggaaac
    10921 cgctcagcgc gggggtcgga tgcagcagac aggccagagt cagtgcgttt tcttgcgaat
    10981 tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta atcaattgtg
    11041 gagaggaagg aacgtgtaac tcactactgc gttcgcgcag tacctctttc atcgcctgag
    11101 tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta cccgcttcgc
    11161 gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg gagctaaaac
    11221 gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcagaacg ccaccatctg
    11281 ccaacggcac atggaagttg taactaaccg ggttttgcgc aatcaaccgt tccagcaata
    11341 cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac accactttgt
    11401 cgacctgcgg cgtggcggta agtgcggcgg cgcgggcaac catctgttca aacgtggttt
    11461 gttccggaat tgcctggcgt tccaccacat tcagcaactg gctgcgggtg aaacggcgtg
    11521 cggaagtttg tttttcctga cgggagaacg actgccagga ttcgggaata tacagcgacg
    11581 aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg atgccctgcg
    11641 ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg ggcgaatccc
    11701 cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa ctgcggtacg
    11761 gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt acttcctcag
    11821 ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa tgattatcat
    11881 ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct tgaggtaacg
    11941 caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga agcattaact
    12001 tcattaataa caggaagctg atttgtgaga ctcgccccgc tctaccgcaa cgcccttcta
    12061 ttaacaggac ttttgctttc aggaatagcc gcagttcagg ccgccgactg gccgcgtcag
    12121 attactgaca gccgtggcac tcataccctg gaaagccagc cgcagcgtat tgtttccacc
    12181 agcgtcaccc tgaccggctc actgctggcg attgatgctc cggtgatcgc cagcggcgcg
    12241 accacgccga ataaccgcgt cgcggatgac cagggctttt tacgccagtg gagcaaggtg
    12301 gcgaaagaac gcaaactgca acggctctat atcggcgaac cgagcgccga agccgttgcc
    12361 gcgcaaatgc cggatctgat tttaattagc gcaactggcg gggattcggc gctggcgctg
    12421 tatgatcagc tttccaccat cgccccgaca ttaatcatca attacgacga caaaagctgg
    12481 caggcgctgt taacgcaact tggcgaaatt accgggcatg agaaacaagc ggcagagcgg
    12541 attgcgcagt ttgataagca actggcggcg gcgaaagagc aaatcaaatt accgccgcag
    12601 ccggtcactg ccattgtcta tactgccgct gcacacagtg ccaatctctg gacgccggaa
    12661 tcagcacaag ggcagatgct ggaacaactc ggctttacgc tggcgaagtt acccgcaggg
    12721 ttaaatgcca gccaaagcca gggtaaacgc catgacatca ttcagcttgg tggggaaaat
    12781 ctggctgctg ggttaaacgg tgaatcgcta tttctgttcg ctggtgatca gaaagatgcc
    12841 gatgcgattt atgccaatcc actgctcgcg cacctgcccg cagtacaaaa caagcaggtt
    12901 tatgcgctgg gaaccgagac gttccgcctg gattactaca gcgccatgca agtgctggac
    12961 agacttaatt cactgtttaa aaaggattta taaaacatac aattagagca ctaacagtcc
    13021 cctcgcccct ttggggagag ggttagggtg aggggaaaat gcacctttag ctaactgtca
    13081 gacgctgtca cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat
    13141 aacaacacgc cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc
    13201 atcatcgctc ccaaaccgcc cagcagcgcc gcgcctatgg catcgcccgt cacgttttgc
    13261 gccgtccaca aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt
    13321 gtgtattgca gcaacgagct gaccgcactc aaccagccga acagcgccag acaaaccacg
    13381 cctaaaatcc acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg
    13441 gagagcagca tcaataaccc tggtcgcgcg ctatgtgcca gcttcccgct ggttaacgcg
    13501 ccaatagccg cgccgagcgg gatcgccgca tagagaaaac caatctgtgc cgccgacatc
    13561 tgccagttgt cagccagtgc cggatacagt acccgcaccg cactcgccat cgtcaataaa
    13621 ccacccagca gcgcaatccc tcccaccagc gggctggcga gcagaaaacg aaatcctgcc
    13681 agtaatgatt tcaacggatg ctcacgcggc tgcggcggcg gtggcagtgc cggaaggctt
    13741 aataacggca gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggcaaca
    13801 ccgccagtcg ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg
    13861 gtcaacatgg tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgcaggtgtc
    13921 gccgccagta gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa
    13981 tagattgcca gcaatgacgg ctccggcagc agcgcattca ggcacagacc aataaaacca
    14041 atgccacagg tgccgcgcgc cagcaaaatc acttttttgc gttcatagcg atccgccagc
    14101 acaccgccga ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaaacccc
    14161 acctgccagg tggaatgtgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa
    14221 cccagagaaa caatagagat gaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt
    14281 ttcaacaggc tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa
    14341 atattttaaa tacctattct taggctgcac atgctaacat atccaaataa gatcgataac
    14401 gataattaat ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc
    14461 cattgccgtg cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat
    14521 tggggcaaaa tcactccccg cttccgtagt gctggaggcc ttctccggca cctgccagag
    14581 cgccgactgc accatcgtgc tcgacgcgcg gctgccgcgt acccttgccg gtttactggc
    14641 aggcggcgcg cttggccttg ccggggcgtt aatgcaaacc ctcacccgaa acccacttgc
    14701 cgaccccggc ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc
    14761 gctgtttggt tactcttccg cacaggaaca actggcgatg gccttcgccg gggcgctggt
    14821 ggcctcattg attgttgcct ttaccggcag ccagggcggc gggcagttaa gtccggtgcg
    14881 tttaaccctg gcgggcgtgg cgctggctgc ggtgctggaa gggctgacca gcggcatcgc
    14941 cctgctcaac cctgacgtct acgatcagtt gcgtttctgg caagccggtt cgctggatat
    15001 tcgtaatctg cacaccttaa aagtggtgct gatcccagtg ctgatcgccg gagcaaccgc
    15061 gctattactg agccgcgcgc tgaatagtct gagtctcggc agtgacaccg cgacggcgct
    15121 gggtagtcgc gtggcacgga cgcagttaat tggcctgctg gcgattaccg tgctttgtgg
    15181 tagtgcgacg gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcatatggc
    15241 gcgctggctg gtgggtgccg atcatcgctg gtcgctgccc gtcacgctac ttgctacccc
    15301 tgccctgctg ctgtttgccg atatcatcgg gcgggtgatt gtgcccggtg aactgcgcgt
    15361 ttctgtggtc agtgcgttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac
    15421 gcgaggtggt gcatgattta cgtctctcgc cgattactca tcacctgttt gctgctggtt
    15481 tccgcctgtg tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaacc
    15541 tcgcagatat tcgccgcgct gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc
    15601 gaatggcgtt taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc
    15661 ggtgcgattt ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt
    15721 aacaccgggg cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg
    15781 gctatcgcgc tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc
    15841 gcctggcgta acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc
    15901 atgctggtgg cctttaatac ctggctgttg ctgaaagcgt ctttagaaac ggcgctaaca
    15961 gcgggtttgt ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc
    16021 gcacccatca ttatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg
    16081 ctggaaatgg gcgatgatac cgcgtgtgcg ctgggcgtca gcgtcgaacg ttcgcgtctg
    16141 ttaatgatgc tggttgcagt ggtgcttacc gctgcggcaa cagcgcttgc cgggccgatt
    16201 tcctttattg ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg
    16261 gggctaaccc aggcggcgct gtgcggggcg ttgttactgc tggcggccga tctctgcgcc
    16321 caacaactgt ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt
    16381 atttacctta tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc
    16441 gtttgcgcgg cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga
    16501 ctgtagaaat acctgatggt cacttcacgg caattatcgg gccaaatggc tgcggtaaat
    16561 ccacgttact gcgtacctta agccgcctga tgacgcctgc tcatgggcat gtctggctgg
    16621 atggcgagca cattcaacat tacgccagta aagaagttgc ccggcggatt ggtctgttgg
    16681 cgcaaaatgc caccacgccg ggagatatca ccgtgcagga gctggtggcg cgtgggcgtt
    16741 atccgcatca accgctgttt acccgctggc gcaaagagga tgaagaagcg gtaacgaaag
    16801 cgatgcaggc cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg
    16861 gacaacgcca gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc
    16921 tcgacgaacc cacgacctgg ctggatatca gtcatcagat tgatttgctg gaactgttaa
    16981 gcgaactgaa ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg
    17041 cctgtcgtta cgccagccat ttgattgcat tgcgagaagg gaaaattgtt gctcagggag
    17101 cgccgaagga gattgtcact gctgaactga ttgagcgcat ttatggtctg cgttgcatga
    17161 tcattgacga tccggtagcc ggaacgccgc tcgtggtgcc gctcggacga acggcacctt
    17221 caaccgcaaa tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca
    17281 tcgcatagcg caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga
    17341 tcacaataat cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat
    17401 atttaaacgg cgtaaaattc acatcgttga tatttgcttt tgttaactgc tcaacaagat
    17461 actgacgatt acgtaattca ccgttcagtt ccgcaacgtc agtgaccgct ttttcaattt
    17521 ccagtttgcg ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa
    17581 cggcctgacc attactgtaa acgggctttt taattcctgc cgcgttggca atgtcgagtg
    17641 aataattgag gcgctgaatg tttgcatcaa gttgattttt cattttaatg cggtcctgag
    17701 ccagtttttc tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg
    17761 actctttcac caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct
    17821 cttcactggt tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt
    17881 ttttcttact ggcattgtca tcaaccgctt tcattttttc gctcaatgcg acaattgcgc
    17941 gatgcaaatc cagttcgtcg attttcgcct cttttaattg gtccatcaca taaggtgatg
    18001 aacgcaggta ctcttccagc aagctaaccg actgaaactt cttgataaac aggttaaatg
    18061 cttctgtacg atcaattttg atatccagat ccagcacacg aagcttagtg aatgttttct
    18121 ccaactcttg ccactgaaca ggttctggag gcgtgacaac cgccgcgctg gtccattttt
    18181 gcggcaggat gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga
    18241 ccgttttttt ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattattac
    18301 tgggcgacgc cagaggatac tcaggaaaat gagcgtcact tccctgttta atattcagtg
    18361 atgacatggg atataacctg aaaatgagtc caataggggg aaatctccac ggcgctcact
    18421 ttaccagctg gtgagaatta tccgaagctg aagtttgtaa attcattcca ctgaatgcaa
    18481 cattcactct atttaagaaa a
//
LOCUS       DAPGRN010000097        18315 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPGRN010000097 DAPGRN010000000
VERSION     DAPGRN010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18315
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.098"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            2..618
                     /locus_tag="RHW33_003486"
                     /pseudo
     CDS             2..618
                     /locus_tag="RHW33_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            721..1224
                     /locus_tag="RHW33_003487"
     CDS             721..1224
                     /locus_tag="RHW33_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV1646958.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRNELRKQFSIKRLNEMEIYPGVTFSDELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1291..2748)
                     /gene="nuoN"
                     /locus_tag="RHW33_003488"
     CDS             complement(1291..2748)
                     /gene="nuoN"
                     /locus_tag="RHW33_003488"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV1646959.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2755..4284)
                     /gene="nuoM"
                     /locus_tag="RHW33_003489"
     CDS             complement(2755..4284)
                     /gene="nuoM"
                     /locus_tag="RHW33_003489"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV1646960.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4448..6289)
                     /gene="nuoL"
                     /locus_tag="RHW33_003490"
     CDS             complement(4448..6289)
                     /gene="nuoL"
                     /locus_tag="RHW33_003490"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV1646961.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6286..6588)
                     /gene="nuoK"
                     /locus_tag="RHW33_003491"
     CDS             complement(6286..6588)
                     /gene="nuoK"
                     /locus_tag="RHW33_003491"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV1646962.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6585..7139)
                     /gene="nuoJ"
                     /locus_tag="RHW33_003492"
     CDS             complement(6585..7139)
                     /gene="nuoJ"
                     /locus_tag="RHW33_003492"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV1646963.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7151..7693)
                     /gene="nuoI"
                     /locus_tag="RHW33_003493"
     CDS             complement(7151..7693)
                     /gene="nuoI"
                     /locus_tag="RHW33_003493"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV1646964.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7708..8685)
                     /gene="nuoH"
                     /locus_tag="RHW33_003494"
     CDS             complement(7708..8685)
                     /gene="nuoH"
                     /locus_tag="RHW33_003494"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV1646965.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8682..11408)
                     /gene="nuoG"
                     /locus_tag="RHW33_003495"
     CDS             complement(8682..11408)
                     /gene="nuoG"
                     /locus_tag="RHW33_003495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV1646966.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11461..12798)
                     /gene="nuoF"
                     /locus_tag="RHW33_003496"
     CDS             complement(11461..12798)
                     /gene="nuoF"
                     /locus_tag="RHW33_003496"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV1646967.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12795..13295)
                     /gene="nuoE"
                     /locus_tag="RHW33_003497"
     CDS             complement(12795..13295)
                     /gene="nuoE"
                     /locus_tag="RHW33_003497"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV1646968.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13298..15100)
                     /gene="nuoC"
                     /locus_tag="RHW33_003498"
     CDS             complement(13298..15100)
                     /gene="nuoC"
                     /locus_tag="RHW33_003498"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV1646969.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15194..15856)
                     /gene="nuoB"
                     /locus_tag="RHW33_003499"
     CDS             complement(15194..15856)
                     /gene="nuoB"
                     /locus_tag="RHW33_003499"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV1646970.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15872..16315)
                     /gene="nuoA"
                     /locus_tag="RHW33_003500"
     CDS             complement(15872..16315)
                     /gene="nuoA"
                     /locus_tag="RHW33_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV1646971.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16946..17884)
                     /gene="lrhA"
                     /locus_tag="RHW33_003501"
     CDS             complement(16946..17884)
                     /gene="lrhA"
                     /locus_tag="RHW33_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV1646972.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT         4568 a         5095 c         4717 g         3935 t
ORIGIN      
        1 tatattattt ctgttttcac tccctctgat tattttttac cacccctggg tcactgcatt
       61 atcgccaaag ccccgacacg taattcctga gcaattagaa aaaacagttc gttatcttac
      121 acaaactgtg catccacgta atgccgacaa tattgataat ctgaataggt ccgtagagta
      181 tataaaagag gtctttgtca gtagcggtgc cagagttacc gcacagagcg tccccgattg
      241 gctattacga ctctgcgcct ggtagccagg attaccctta cccgcaatgt cctggcttta
      301 tcccgatcgg ggggacttta ttgcctggtc ggcagaatac aggatatcaa cgccgttcgt
      361 caggtaaaaa cggcattgtt atcatcccgg gatttatctg tttattctat gaatgcccca
      421 tggtttattc acggtattga tttctctgac caccttaatt attggcaaca cgatattccc
      481 gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt gcccggtgat
      541 accgcagaca gattgaatta tcagaaaatg gctcaggtag tggatggtgt tataactttg
      601 ttatacaaca gtaaataata aaaatttctc gaggcgggtc cagaaaaagc gacatgcaca
      661 ttttattttc cctcatctgt tttaagatgc gaaacatgaa attcaacgga ggaaaaataa
      721 atgggaatga ttggctattt tgcagaaatt gactcagaaa agattaacca actgcttgag
      781 tctactgaaa aacctttgat ggataatata catgacacgc tttcaggatt acgccgactg
      841 gatattgata aacgctggga ttttttacac tttgggctga caggcacctc tgcttttgat
      901 cccgcgaaga atgatccgct gagtcgtgcc gtgcttggtg aacacagcct cgaagatggc
      961 atagatggat tccttggatt gacctggaat caggagttag ccgcgacaat cgaccgactg
     1021 gaatcactgg atcgaaatga actgcgtaaa cagttttcca ttaagcgact caatgaaatg
     1081 gagatttatc cgggagtgac cttttcagat gagttagagg ggcaactctt tgcttcgatc
     1141 atgcttgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca agggaatcat
     1201 gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg ccgaacatgt
     1261 cggcggcttt ctgacttaca aactaacaga ttacatcagt ggcattgcta accgcacaat
     1321 gctaatcagt ggttgtggcc atacacccag caccagtacc aacagtgcag agatcagcac
     1381 cacgataccg cccgcgctgt actgccagtt tgacggcgca tcgcgacccg gttgttccgg
     1441 ggcgtgaaga tacaggctca ccgccacgcg caggtagtag tagaggccga ttgccgaacc
     1501 gacaaccacg gcacccacca gccaccacag gtgtgcctgg acaccgaccg ccagcacgta
     1561 gaacttaccg ataaagccca gcgtcatcgg gataccggcc agagacagca tcatcaccgt
     1621 catcaccgcc gcgaggatcg gacgatgcca gaacagaccg cggtaagaga acagggaatc
     1681 agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg cgccgaggct
     1741 gctgaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct cgccagtttg
     1801 cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt aaccgagcag
     1861 acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg aggcaaaggc
     1921 gataatcgcc agcaccacac gaatcgcttc gctgtcaccc accggtgcgt agaggaacag
     1981 acgcatcact acaccgaaga tagcgatttt gctcgccgtc gccaggaaag tggaaaccgg
     2041 cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca gagagagttt
     2101 gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct cgtttagcat
     2161 accgtcgccg aggtttttac ccagcgcgac aaacgacagg tcgccagact gcgcatacac
     2221 cagcgccata ccgaacagca ggaaagaaga cgctgcggca gaaaggatgg tgtatttgat
     2281 actggcttcc agcgaacgtt tctggcggaa agcgtaaccg accaggccaa acagcggcaa
     2341 agagatcagt tcaataccga ggaacagaga cgccagatgg tttgcattcg ccagcaggat
     2401 cccgcccagc gcggcaatta acaccaacag gtagaactca tccttgttgt cgttatagcc
     2461 ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata ccagcccggt
     2521 gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag cgcccgcctg
     2581 gccaacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga gcgtagcgtt
     2641 gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg tcaagccgac
     2701 gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg cgatttacgg
     2761 ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg cggagtgcga
     2821 ggtatccaga atcggctgcg gatagaagcc cagcagtacc agcagcacca ccagcaacag
     2881 gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa tctggctttt
     2941 cgctttaccg aagtaagcgc gatgtaacat cgccagcgaa taaacagatg caaagaccag
     3001 cccaaaggta gaaataacgg taatcaccgg gacaacctgg aagctgccga acagaatcat
     3061 aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg caaagaacag
     3121 cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc gcatgtcgcg
     3181 ggtatggata cgttcataaa gctgaccgca gagaataaac agacccgccg ccgacaagcc
     3241 gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg tgtagatagc
     3301 aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt tgatatcggt
     3361 ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc acatagcgat
     3421 tggcgcgaac tctgccgacg cgttcgggaa cagcggtagg gagaaacgca gcagaccgta
     3481 agcggcggtt ttcagcaaga tccccgcgag gtccacggaa ccggcggtcg gagcctgtga
     3541 gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttcaccg caaacgcgat
     3601 gaagaagccc agcatcaaca gatattccac accactggac attggcgtat tcaacagctc
     3661 ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca gcgccaggat
     3721 ggcgatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg ttgccgccgt
     3781 gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga agtacatcgg
     3841 caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa ggaacacgcc
     3901 gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat atttttcgat
     3961 ctctttccac gaacagagta ccgccagcac accgagcaga ccggtcagca cgaccatcag
     4021 cagcgacagc ccgtcaatgg cgagatggat agagatacca aaacgcggga tccacggcat
     4081 gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat aaccgccctg
     4141 caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg cgatccagcg
     4201 cggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccga taaagggaat
     4261 taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc ccgtcaggac
     4321 cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt agtgtcggat
     4381 gcaccgcgaa ggaggcttat ccgacctacg ttcttatatg cccaggggca atcccacaat
     4441 ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg atgctcatgg
     4501 atgccacata ccagcgcaga tagccgttct cgcttaacag cagaccttta cctgcaaagc
     4561 gggaaagaac cgccggaatg ttcatcattg agttcagcgg atcgcgtttc agcaaccagg
     4621 caatacccag gaacggcttg acgaacactt tgtcgtacag ccagtcaaag ccccaggcgt
     4681 tgtaccacca ggtgcccagc agacggcccg gcgcactgtt ggcgatggag gtcaccagag
     4741 tacgtttacc cagccacagc caggctgcca gcagaatgcc gaccaccgcg accacaccag
     4801 aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc ggaagcacgc
     4861 cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc agcacaatca
     4921 gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct tttccgtgga
     4981 agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg accagacctg
     5041 ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca tccttactga
     5101 agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc aggaagcaga
     5161 gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc tgttcgtgat
     5221 ggcaggccag aatgacagaa ccggatgcca ggaacagcag cgctttaaag aacgcgtggg
     5281 tcatcaagtg aaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg aacatgtagc
     5341 caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt accagcgcgg
     5401 caaaaccggc cagcagcagc gtaaccgccc caacaatgcc caccagatgc agaacttccg
     5461 gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg gttaccatcg
     5521 ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg gcaagccatg
     5581 tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc agcgtcgccc
     5641 acatcagcat attattgccg tcagcaaagt gcgctggtgc cagttccacc atttcgcgga
     5701 agttcagggt gcccagttcg ttgtaaagaa tgaacagcgc gaaagcgagg aacacgtcac
     5761 ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga tcggtgtaat
     5821 agaaaccgat cagcagatag gagcataggc ccacgccttc ccagccgagg tacatcagca
     5881 gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg gtgtaggcga
     5941 agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg tggatcagga
     6001 aacccacacc agtaaccacc gagagcatgg tcagcgacag gccatccaac accaggttaa
     6061 aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg taagcctgct
     6121 cgccgttagc gaagaagtcg acgccaataa aggcggttac cagcgccgcc aggcccacag
     6181 agcctacgcc gacgatcgcc gagacgtttt cagaccagcg cccacgggag aacgccagca
     6241 ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc cgcgcatctc
     6301 acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca gcagcgcaag
     6361 gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca cctgaccgtc
     6421 ggtctggccc cagtagcttc cggcgaccac aaaggccagc gcggaggcgt taatcatgat
     6481 ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg ttaagccaag
     6541 aacgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc gtgctcctcc
     6601 gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc acgctcttca
     6661 cgaccgacgt ggaaggccac aaccagaccc gcgagcagca gcatagaagc cagttccacc
     6721 gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat tggcgtaccg
     6781 tcgataccct gatcattaac accaaggatg gcgtaaacaa tcaccaccag catgatggcc
     6841 gacaaaatcg ccggaccaat ccacacctgc ggtttcagcc actggcgttc ctgttcgatt
     6901 tctgaaccgc ccaggttgag catcatcact acgaacacga acagcaccat aatggcaccc
     6961 gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa gaacaccccg
     7021 gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt ggtatgggtg
     7081 atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc gaactccatt
     7141 gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg ttctctgctt
     7201 cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta tattccgggt
     7261 atttgcccgg accggagatc agcagatcct ctttctcgta aaccagatcc tggcgcttgt
     7321 attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag gcttcttcgc
     7381 acaggccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga taccagcgac
     7441 cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct accgcgcaga
     7501 ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg atacgaccac
     7561 gataacgggg cggcagatag accggctctt ccgggtacat tcgcgtttcg cgtttggcga
     7621 acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct actaacaatt
     7681 cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga cagccgccgt
     7741 taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg acattacctg
     7801 gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca taaagaacgc
     7861 ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc caccgaagaa
     7921 cagcgtcacc atcagtgcag agatggtcac aatcccgatg tattcgccca cgaagaacag
     7981 accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct gctcggcttc
     8041 cggctggtca aacgggtgac ggtgacacac cgccacgccc gcgatggcaa aggtaataaa
     8101 accaaagaat tgcgggatga cgttccacac atgcgcctgg ctgttgacga tgtcggtcat
     8161 gttgaatgaa ccggcttgcg ccaccacgcc catcagggaa agcccgagga acacttcgta
     8221 gctcagggtc tgcgcagaag cacgcattgc acccagcagc gagtatttgt tgttacttga
     8281 ccagcccgca aacagcaccg cgtaaaccgc cagaccggcc atcatcagga agaacaaaat
     8341 cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg caaaggccag
     8401 cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat ccgagaattt
     8461 cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct ggagcgaacc
     8521 gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca ggcgacgttc
     8581 gccaaagctc atgaatgccc cgcaggtgac aaccaccagt aggatcacca ccgctttgag
     8641 gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg tgcctccttg
     8701 agatcctcaa gatgcgcgcc agccagcacc ggagcaatgc cggacatacc catcggcaag
     8761 cccacctgcc ctgccgtcag cccttcggcg atttcaaccg gcagcgtgac cgtgttgcca
     8821 tcgtaactaa aggagacgcg tgtacctgcg ttcacaccca acttcgcggc atccgctggg
     8881 ttgagtttga tgtacggctg cggcatacga ctctggaaga ccggagcacg ctgtgacaat
     8941 tcatcgctgc caaacaggtg gtagtacggc gcgatacgcc atttcccgtc ctgcggctgg
     9001 aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc aaacagacgc
     9061 acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt gttccacgcc
     9121 tgcggggagt tccagcctgg cgcccaggca aacggcactt gcgaacggtg cgcagtcggc
     9181 tggttgttac cttccatcga gaaggtaaac atggtgtcaa tatcctgcgg ctgacgtggc
     9241 tcatgaacgc taatattggc gcgcatggcg gtacgaccgc tgtaacggtg cggttcacgg
     9301 gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt gatacctgcc
     9361 agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt ccagtccact
     9421 tcacggctca acagggtact gtgcagcgag tgtaaccagc gccagctttc cagcatgaca
     9481 gttttgctgt cgtaataagc cggatcgtaa acctggaaga aacgttgggc gcgaccttca
     9541 ttattaatta ccgtaccgtc gctttcagca aagctggcag cggaaagtac cagatgggcg
     9601 ttttccatga tcgctgtgcg ttgatggtca accaccatca ccagcggcgc tttagccagc
     9661 gcagcattca cgcgggtagc agaagcgtga cgatgcagat cgttttccag caccaccacc
     9721 gcgtcggcgc gtccggtttc cagttcggtt aacgcttctt caagcgatcc accgcccata
     9781 atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccgac gtcagcgccg
     9841 cgacctttca gggctttcgc gacgttagcc gcagcctgaa ttacttcagc gctaccggcg
     9901 ttcgtcccgg agataatcaa cggtttcttc gcacccgcca gagcctgcac gatgacgtcg
     9961 attttgcttt gcagctcagg ctcgataccg tcaactgctg gtgcggagtt atccagcgca
    10021 tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta agtccacgcc
    10081 gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg cttagcacgt
    10141 tgaccgatat tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc tgccgccatt
    10201 tcgcgcgctt tacctttcac cgcctgacgc actgccagcg cgacgcgcgc gccggtctgg
    10261 gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg cagagccgga
    10321 gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc ctgctcaccg
    10381 tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc aaagttactt
    10441 tccacgctgg cgcgcggaga accaatgccg atcactttct tcgactgacg cagaatatct
    10501 gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc acgacgctgt
    10561 actggctgac gcggacgatc tttcaggttg acgtaaccgt aaccgaaacg accacggtcg
    10621 cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg cagttcgccg
    10681 taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat gctcggcgca
    10741 aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt aaatacgccg
    10801 gtcgggcaaa tttcgaccag gttaccggaa aattcgcttt ccagcgtgcc gtcttccggg
    10861 cgaccgaagt agacgttgtc gtgtgcgcca taaacgccca gatctgtacc gtcagcgtaa
    10921 tctttgtagt aacgcacgca gcggtagcag gcgatgcagc ggttcatttc gtgagagatg
    10981 aacggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg acggaagctg
    11041 tgtccggtca tcacggtcat atcctgaaga tggcagttac cgccttcttc acataccgga
    11101 cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa ctgtttcgct
    11161 tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca ggacatcacc
    11221 aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg gcggcaagca
    11281 ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc cagagacaga
    11341 caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc tacatgaatt
    11401 gtagccatta gcatgcttcc agttttctca gtcagagact gagcgttaat cgaaattcgg
    11461 ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg tattgctgaa
    11521 cggctgtttg atccccgcct caaattcttc gcggaaatat ttgatggcgc tctgtaacgg
    11581 ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga atcgacacag
    11641 ttgctcaagt gtttcgatat cgcccggctg accttcgccg cgctccagcg cacgcagaat
    11701 tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg actcacgggc
    11761 gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt caaccgccat
    11821 agccagcgcc gtacccagac ggcttcccgc tttaccgata ctttcgaatt ccatcggcag
    11881 gtcaaggtgc gcttcggtca ggaagtcggt gcccgcgccg cctggctgcc aggctttgaa
    11941 tttcagaccg tcacgcatac caccggcgta atcttcgagg atctcgcgcg cggtagtacc
    12001 aaacggcagt tcccacaggc ccggattttt cacccgaccg gagaagccca tcagcttggt
    12061 gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag cgaggatcgc
    12121 cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttacccc atgcgccgga
    12181 ggtcgccggg aaaggtggct tcgagcgtgg gttagcgcga cgcccttcca gggagttgat
    12241 taacgctgtt tcttcaccgc agatgtagcg ccctgccccg gtatggacga acagttcgaa
    12301 atcgaaacct gttcccataa tgtttttgcc gagcagccct gcttcggtgg cttcggcaat
    12361 ggcacggcgc agattcactg ccgcttcgat atattcgcca cgcaggaaga tgtagccacg
    12421 gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt gcggcagttg
    12481 ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt tacacagcag
    12541 gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc cagtcgaaaa
    12601 gcccgcgccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga tttcgtccgg
    12661 agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc ggtattcgtc
    12721 cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg tttcgggagt
    12781 acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc cggggtcaga
    12841 tgcgcgtgag tgtcctcatc gatcatcata tttggccctt tatcacagtt ccccaggcag
    12901 caagttggca gcagcgtaaa gcgaccgtca aatgtcgttt gccctggttt gatgtttagc
    12961 tttttctcga gcgccgcctg aataccctgg taaccgttga tatgacagac cacgctgtcg
    13021 caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta gaacgttgcc
    13081 acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg gatcgcacca
    13141 tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga cgccgcacgc
    13201 gggtcttcgt agtggtgcat ctcgtgctcg atcgcttcac gctctgccgc actcagctca
    13261 aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca tctgacataa
    13321 caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg cggatcgccg
    13381 ccggaatttg ctgcaaatgc gcataactcg gcgtacggat acgagtgcga taactcatgg
    13441 tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca atcatctgga
    13501 aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg gtgatcaggg
    13561 tttcgatatg ttgcagcgtg cgctctttcg gcggtggcgt ggtcagcggg tgatccgctt
    13621 tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga ctctggcgca
    13681 gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacca ccaaccggga
    13741 tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg tcgaagtcga
    13801 tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct ttcgcgccat
    13861 aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc gctttctcgt
    13921 aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga tcccagccac
    13981 gcggcaggtc atgcgctaca ccgccaatac ggaaccacgc cgggtgcata cggaaacccg
    14041 tgattgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag aacacgggcg
    14101 tcattgcgcc gacgtcctga ataaaggtcg agatatacag caggtggctg ttgatgcgga
    14161 acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg atcccggcca
    14221 gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg tattcgatac
    14281 ggtcggtata cggaatgtag ctgtgccagg actggcgttc gcccattttc tccgcaccac
    14341 ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccatcg agttgcaaaa
    14401 cgatacggaa agcaccgtgc gccgacgggt ggttcggacc gaggttgagg aacatgaagt
    14461 cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc agggcttcca
    14521 tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta gcgcgcgccg
    14581 ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg cggcgcaggt
    14641 tcgggtgacc gtcgaatgta atgccaaaca gatcccaggt ttcacgctca taccagttag
    14701 cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc agcgccacct
    14761 tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg gaaaaatccg
    14821 cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt aagtcaaaca
    14881 gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt aattgttcac
    14941 gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag gcatccggcc
    15001 caaaacggtt gcgcagttcg ccaattaccg gatcatcaag atgatcgcgg gtctgccagg
    15061 cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt ccgtggtgac
    15121 tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa atccctttgc
    15181 cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca atgcgttcac
    15241 cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga tcgccaacca
    15301 cccaggagag cggacgacgt tctttaccga tagattcctg caacagcatc agtgcctgca
    15361 tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg atgaatttat
    15421 cgacgccctg cacaacggaa taaatatcgt acataccgcc agagttggca caggcaccca
    15481 ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata accggtgcca
    15541 ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc gaagcacgca
    15601 atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc accatctcaa
    15661 cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga ccccagttaa
    15721 ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc agagggtcgg
    15781 ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga tctatgcggg
    15841 tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg atactgttcg
    15901 tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc gcgccaatac
    15961 gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct tccacaaagc
    16021 ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc gcttcaacgt
    16081 cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt aagcgggcgg
    16141 agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg cgtgcgcgac
    16201 cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca acgataagaa
    16261 agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata ctcattgctt
    16321 actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc cacatcgatt
    16381 catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat acagctaatt
    16441 gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag tttcttcaca
    16501 ttagtttaca taatatcaac accattagca tttaatgccc tttcacccca gatccttgac
    16561 gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg gaaaaaatac
    16621 gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt attttcttga
    16681 tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta cgtatttttt
    16741 aacatcattg tagcaggtga tttttttcag gcgattattt gtgcgtacgg ggcgtgaatc
    16801 tctggcgatt gaaaaatgaa cagttttgta cgttctgcac gatgtgaaga aggttcttta
    16861 ctaaaaaaaa gccgctgggg tttaaaacac ccccagcggc tcgtttttta cactattgtc
    16921 tcaggaatta tctatcgtcc gtcgattact cgatatccct ttcaatcaac aatgaatcat
    16981 ccccttccgg agcagatatt gggctgtatt gccacggatt atggtagctc tccatcgcct
    17041 gatagatcac ctgggccagc tcattattac tggacgggtc atagcacagc aaatattcgg
    17101 tatcaggtag cggcggtaag ccatctactc cactcagcac acgcaagtcc ggactcatca
    17161 tctcgaccgg ccttgccgtc acgccaagac cggctttcac tgccgcacga acggccggaa
    17221 gcgtcgaggc gacataagcc agtcgccagg gaatatctgc tttattaagc gtcgccagca
    17281 ccatatcgcg aaacggactt ggatcatcca gcaatacaag agggatcggc tcaccttttt
    17341 gcaatacgta ttccgctgcg cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct
    17401 taaacgctga aggacgatgg gtggttacca tcaaatccac ttcctgggat tccagcattt
    17461 ctgccatata ggcattacgt ttaacgcgga catccagcgc taatttcgga taaaccgaac
    17521 tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag
    17581 ttaacacgcc ctgaagatta ctgaacatta atgatgagca ggcctcatca ttaaaacgca
    17641 ggattttcct ggcgtaacca agaagttgaa tgccatgttc agttaacagt ttgttgcgac
    17701 cgtgacgagc gaacagttct ttcccaacga gttgttccag acgctgcatt tgctgactta
    17761 cggcggactg agtacgacac acagcggcag ctgcggcagc aaaagtgttc agatcggcaa
    17821 cagcaacaaa tgttctcagc agatcgaggt cgaggttaat tatcggacga tttgcactta
    17881 tcatatatta tcacttactg gcggctcata ctgagctggt taatgctgtg cacacacaaa
    17941 caagcaattc catttgtaat gtgcctccct ggcagtttca tccggaaccc ggacgaaagt
    18001 aaaaatgcat atgagttgca ctaaaaaagc gactcacatt gttccgttat aatgcctgaa
    18061 gtagatcaca gaatatatct tcagggatcg catatctatt aagttactca ctcttttcta
    18121 tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca
    18181 gcatcgctgg tcatacgcga acacgtacaa acagcaatgg tgaggcggca tcaagagcag
    18241 ggatccgtca tttatgcgag cattttaccc caaaaacctt ttatttataa gggtcattgc
    18301 gaattatctg atgca
//
LOCUS       DAPGRN010000098        18221 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPGRN010000098 DAPGRN010000000
VERSION     DAPGRN010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.099"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(272..919)
                     /gene="ychE"
                     /locus_tag="RHW33_003502"
     CDS             complement(272..919)
                     /gene="ychE"
                     /locus_tag="RHW33_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV1646973.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1396..4071
                     /gene="adhE"
                     /locus_tag="RHW33_003503"
                     /gene_synonym="adhC"
     CDS             1396..4071
                     /gene="adhE"
                     /locus_tag="RHW33_003503"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV1646974.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4372..4989)
                     /gene="tdk"
                     /locus_tag="RHW33_003504"
     CDS             complement(4372..4989)
                     /gene="tdk"
                     /locus_tag="RHW33_003504"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV1646975.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5593..6006
                     /gene="hns"
                     /locus_tag="RHW33_003505"
     CDS             5593..6006
                     /gene="hns"
                     /locus_tag="RHW33_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV1646976.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6150..7058)
                     /gene="galU"
                     /locus_tag="RHW33_003506"
     CDS             complement(6150..7058)
                     /gene="galU"
                     /locus_tag="RHW33_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV1646977.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7260..8273)
                     /gene="rssB"
                     /locus_tag="RHW33_003507"
     CDS             complement(7260..8273)
                     /gene="rssB"
                     /locus_tag="RHW33_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV1646978.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8365..9270)
                     /gene="rssA"
                     /locus_tag="RHW33_003508"
     CDS             complement(8365..9270)
                     /gene="rssA"
                     /locus_tag="RHW33_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV1646979.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9383..9841
                     /locus_tag="RHW33_003509"
     CDS             9383..9841
                     /locus_tag="RHW33_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV1646980.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9891..10733
                     /gene="purU"
                     /locus_tag="RHW33_003510"
     CDS             9891..10733
                     /gene="purU"
                     /locus_tag="RHW33_003510"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV1646981.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFNIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10893..10977
                     /locus_tag="RHW33_003511"
     tRNA            10893..10977
                     /locus_tag="RHW33_003511"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10927..10929,aa:Tyr,seq:gta)
     gene            11024..11155
                     /locus_tag="RHW33_003512"
     ncRNA           11024..11155
                     /ncRNA_class="other"
                     /locus_tag="RHW33_003512"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11339..12016)
                     /gene="narI"
                     /locus_tag="RHW33_003513"
     CDS             complement(11339..12016)
                     /gene="narI"
                     /locus_tag="RHW33_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV1646982.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12016..12726)
                     /gene="narJ"
                     /locus_tag="RHW33_003514"
     CDS             complement(12016..12726)
                     /gene="narJ"
                     /locus_tag="RHW33_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV1646983.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12723..14261)
                     /gene="narH"
                     /locus_tag="RHW33_003515"
     CDS             complement(12723..14261)
                     /gene="narH"
                     /locus_tag="RHW33_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV1646984.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14258..18001)
                     /locus_tag="RHW33_003516"
     CDS             complement(14258..18001)
                     /locus_tag="RHW33_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV1646985.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDANKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4614 a         4746 c         4347 g         4514 t
ORIGIN      
        1 ttaacaattg attattattt agcactttcc tctaccaaag gcagtggaat tactaatatc
       61 atttcaatta ttcttcagca agcccaggat tatgatgtgg cgaaaataac atgatggtga
      121 ggagaagcag ttaaggaaat ccttataact aactgtaatt tgagaagaat atttttacct
      181 attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg aagtaaacgg
      241 agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg aaaatcccct
      301 taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc ataatacgcg
      361 taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc atgcggaaca
      421 atccccaaca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac agatagctaa
      481 tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc cccgccatca
      541 acggcaacgc cagtggcacc acaccaatgc tttcacgtac cgcggtttct gatttttctt
      601 gcttgttctg tttatcctct ccaagcttgc cgctgatcat cgacatcgct atcgtcacca
      661 ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt tgtagaatcg
      721 tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg gctgtaaggt
      781 tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag acgggaataa
      841 tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa acgggaaaat
      901 caaaaagggt ctgaatcacg gttagctccg aagcaaaagc cggataatgg tagccataaa
      961 taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac ctcctaacta
     1021 cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct aatgtagcca
     1081 ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaaacga tgctgaaagg
     1141 tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag tgagtaatct
     1201 tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt aaactctttt
     1261 agtaaatcac agtgagtgtg agcgcgagta agcttttgat tttcatcggt taagcaaatc
     1321 atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa agtttaacat
     1381 tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact cgtagagcgt
     1441 gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga caaaatcttc
     1501 cgcgccgccg ctctggctgc tgcagatgct cgaatcccac tcgcgaaaat ggccgttgcc
     1561 gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc ttctgaatat
     1621 atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga cgacactttt
     1681 ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc gaccactaac
     1741 ccgacttcaa ctgctatctt caaatcgctg atcagtctga agacccgtaa cgccattatc
     1801 ttctccccgc acccgcgtgc aaaagatgcc accaacaaag cggctgatat cgttctgcag
     1861 gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca accttctgtt
     1921 gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc gactggtggt
     1981 ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggcgt aggcgcgggt
     2041 aacactccgg ttgttatcga tgaaactgct gatatcaaac gtgcagttgc atctgtactg
     2101 atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt tgttgttgtt
     2161 gactctgttt atgacgcagt acgtgaacgt ttcgcaaccc acggcggcta tctgttgcag
     2221 ggtaaagagc tgaaagctgt tcaggacgtt atcctgaaaa acggtgcgct gaacgcggct
     2281 atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt accagaaaac
     2341 accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt cgcacatgaa
     2401 aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc ggtagaaaaa
     2461 gcagagaaac tggttgctat gggtggtatc ggtcatacct cttgcctgta cactgaccag
     2521 gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacggc tcgtatcctg
     2581 attaacaccc cggcttctca gggtggtatc ggtgacctgt ataacttcaa actcgcacct
     2641 tccctgactc tgggttgtgg ttcctggggt ggtaactcca tctctgaaaa cgttggtccg
     2701 aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt gtggcacaaa
     2761 cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga tgaagtgatt
     2821 actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa caatggttat
     2881 gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt cttcttcgaa
     2941 gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg cagaactggc aaactccttc
     3001 aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc gaagatcatg
     3061 tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg ctttatggat
     3121 atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat gattgctgtc
     3181 accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac tgacgacgct
     3241 actggtcaga aatacccgct ggcggactat gcgctgaccc cggatatggc gattgtcgac
     3301 gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct ggacgcagta
     3361 actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga tggtcaggct
     3421 ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga agggtctaaa
     3481 aatccggtag cgcgtgaacg tgttcacagt gcagcgacta tcgcgggtat cgcgtttgcg
     3541 aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac tgggttccca gttccatatt
     3601 ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa tgcgaacgac
     3661 aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg ccgtcgttat
     3721 gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc tgctaagatc
     3781 gagaaactgt tggcatggct ggaaacgctg aaggctgaac tgggtattcc gaaatctatc
     3841 cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact gtctgaagat
     3901 gcattcgatg accagtgcac cggcgctaac ccgcgttacc cgctgatctc cgagctgaaa
     3961 cagattctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac tgcagcgaaa
     4021 aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta atcagtagcg
     4081 ctgtctggca acataaacgg ccccttctgg ggccgttttt ttgtttaccc aaagcaactt
     4141 ttccataaac cgacagcatt agccttcatc atatttgcga cgatgtataa cgcctaaaca
     4201 cagggatatt gtactttaca ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc
     4261 gtatagcggc gttaaaaact gccgagaagg gtgtatagcc cggaagaagt gcgtaaaacg
     4321 aactgacagg ataaaagtgc cctgctcacc ctgtcagtaa agaaattctt attaatcgtg
     4381 gcgatgcctt tcctgaatag ccgttaatga gccgacttgt aacgcctctt tatagtgttt
     4441 acggcataca gaaacgtatc gttcattacc accaataacc acctgctcac cttcgttata
     4501 aggtctgcct gcttgatcaa gacgcagcac catgcttgct ttacggccac aaaaacagat
     4561 ggtttttaat tcaaccagtt tgtcggacca tgccagtaag tattggctgc caataaataa
     4621 ttcacctcga aaatcggtac gtaaaccata acaaagtacg ggtatatcga gttgatcgac
     4681 aacctccgat aattcatata cttgttgtct ggttaaaaac tggcattcat caaccagtac
     4741 gcaatgaatt gcctgctgtt catgttccgc acgaatctca tcaaataatg atgaattttg
     4801 gttaaataat tttgcaggcg atgacaaacc tatacgcgaa ctgactttcc cggcaccaaa
     4861 gcgatcatca atttctgccg tatatacgac agtgcgcatg ccgcgttcct ggtaattgta
     4921 tgaagattgc aacaatgctg tagacttacc cgcattcatt gcggaatagt agaaatatag
     4981 ctgtgccatc agccacaggc cctcaatgat atgaaataaa gttggctgga gtttatcata
     5041 attcgtcagt ttttcagctc aacagtatgc acagaagtgg tatatgcgtt ctcccttacg
     5101 aagccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta taagcgggta
     5161 aatgactgct ggtaactatt cacaatcttt aacctgttgc gcaagtaata gccctctgtt
     5221 gacctccagg agatagtgca atactaagtc cctgctctta ttgcgactgt tctactcttc
     5281 atcattcgct taatagggaa ttctcgtaaa cacgactaat acagaagact gaaaggtcgt
     5341 cagcctacga taatctcccc ataaaatgtg acatgaatca ggaagtttta acctcacgag
     5401 ctgcgaaatc atcggtacaa atagggctat atgccgcgtc ttttctggct aattttatga
     5461 aaagatattt attggcggca caaaataaag aacaattttg aattccttac attcctggct
     5521 attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca atataagttt
     5581 gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac tcttcgtgcg
     5641 caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt
     5701 gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga gcgtactcgt
     5761 aaactgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa cgaactgctg
     5821 aatagccttg ctgccgttaa atctggcacc aaagctaaac gtgctcagcg tccggcaaaa
     5881 tatagctacg ttgacgaaaa cggcgaaact aaaacctgga ctggccaggg ccgtactcca
     5941 gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag
     6001 caataatctt ttgtagattg cacttgctta aaatcccgcc atcggcggga ttttttattg
     6061 tccggtttaa gacaatttaa taagaataag ctataaaaaa acggcgtcga ttgctcaacg
     6121 ccgtttcgtg gataacaccg atacggatgt tacttcttaa tgcccatctc ttcttcaagc
     6181 caggctttaa attccgtgcc aagggtgtta tgacgaatac cgtattcaac gaaggcctgc
     6241 atgtaaccta atttattacc gcagtcatgg ctcttccctt tcatatgata ggcttcaacc
     6301 gtttcttttt cgattagcat atcaattgcg tcggtgagct gaatttcatc accagctccc
     6361 ggaggggttt ttgccagcaa cggccaaata tccgcactaa gtacgtaacg acccacaata
     6421 gcgagattag acggcgcaac atccgctttt ggtttttcaa ccacgccaac catcggtacg
     6481 ctttcacccg gcgctaattc aacgcctttg caatccacaa cgccatatgc ggtcacatca
     6541 gcaaccggtt caaccatgat ctggctatga cccgtttcat cgaagcggcg gatcatctct
     6601 gccaggttat cctgtgacaa atcggattca tattcatcca gaataacgtc aggcaaaata
     6661 acagccacag gttcatcacc cactaccggg tgagcacaca ataccgcgtg tcccaggcct
     6721 ttcgccagcc cctgacgaac ttgcataata gttacgtgcg gcggacaaat agactgcact
     6781 tcatcaagca gttgacgttt tacacgtttt tccagcattg cttccagttc aaaactggta
     6841 tcaaagtggt tttcaataga gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg
     6901 ccagccgcaa tacattcatt cacgacgtat tgaattaatg gcttatcgac aagtggcagc
     6961 atctctttcg ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg
     7021 ataacggctt ttttgacttt cgtattaatg gcagccattt aaattctcct ggactgttcg
     7081 tgttttgaac gtgttcatat ttctgtatcg catcccagta tatcagcacc ccggaggggc
     7141 taagttctga aaaggatgcg agttagcata attaagacaa ttacttatac gaatggcgac
     7201 gatagtacca ccagccgaca ttagctggta atgcaaattt agcccgcgtt atcgtttgct
     7261 cattctgcag acaacatcaa gcgcagccga ccaccggttc cccatatttg gcattgccag
     7321 gcatcgcatc gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc taacggaaca
     7381 ccattactga tttgcacctg gtgttcgccg gtatttaacg tcgcattcag acccgcagaa
     7441 accagaatga gatttttcag ttcgcgatga taatagccaa ctaatagcgg aaactgcccc
     7501 ggcagattgg cctgacggag taaatggttt acctgcttca ataacgcgcc caactctggc
     7561 aaccgttgat tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat
     7621 aacaaggcag caagtacgcc attatgtcca gctcgggtga catcaaggca ataaaatgcc
     7681 agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc
     7741 aattgacgat aattaacccg gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc
     7801 tgtaataatt tcgccgctgc ggcagggtta tcaaccattg catcccagtc gcgaaaaagc
     7861 ctttcctctt cctcaacgcg cgaattaaac atgctgggat agagacaggc aaaaaccatc
     7921 tcgcgcaagc gattcagatc tttaactggt ttcagcaaaa catcttcaac gcccagacgt
     7981 aacgctttgg caatatctgc catattttca gtggcagata tcaccagaac tggggtctgg
     8041 tcgcctctgt tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg catcgcgata
     8101 tcacatatca tcaggtctgg agtgaaacct cccagcaact caagggcatc caccccatca
     8161 gccgccagta ccgttgtcgc tcccaatgag gaaaaccacg aatccagaag cgagcgaaat
     8221 acctgctcat cttcaacaat gagaatctgt tttccgacca atggctgcgt catgttctct
     8281 cccctgactg gctttactca atagtggcat gctattgcca cctgcgcctg tcagaattaa
     8341 cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt accaacggca aaagttcgtc
     8401 cattttcttt tccactgcca gctgcccggc cgcaatggca gcgtgcgcac gatggaaatc
     8461 aagcgtagat atttgcgggc aaacaggttg aatcagaata tcgggcggat cacctgccat
     8521 gcggttcctt ttaaggcggt tctccagcac ctggatagaa gtggtcataa tctctgttgc
     8581 cgttggtgct gtcaccgccc gacgtgtcgt tatactgcct aacctttctt tcagacgcgc
     8641 atgccacggc agagaatcac cattctcgct attttcttca ctgacattaa aggagagcaa
     8701 atcttgttgc atcaaatgag catcgtgctg caggtcgacc gctatcacaa tatcagcacc
     8761 caatgcacgc gtgagcgaaa taggaattgg gttaacgact gccccatcaa ccagccagta
     8821 gccgttatgc gcaacgggtg ccattagtcc tggaatactg catgatgcgc gaatagcaag
     8881 atggagatcg ccttcagtaa accataattc acgcccagta cttaaattgg tggcaacagc
     8941 cgcgaagcga cgggaacaat tttcgatctc tgtttccggc attatttcgc ggtattgatt
     9001 gaagacacgc tcgccacgca gtaacccacc gcgctgccag gagagatcca tcaggcgtaa
     9061 aacatcccaa taactgaaag aggtcaccca atcttccagc gcagataatc gatcgcatgc
     9121 ataggcagcg cccaccagcg aaccaattga acatcctgca acgatatcaa tttcaatacc
     9181 cacttttttt agcgcattaa taacgccaat atgcgaccaa cctctcgccg cgccagatcc
     9241 cagcgccagc cctattttta tctttctcat tattcccgca agattcccct ggaatgcaat
     9301 cgtagccaca tcgctactgc tcggttaaca tagtgctact caggcggtta atacgccgtt
     9361 attgtttcca gggagattat ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta
     9421 tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct
     9481 catgcgttcg cgttactgcg cttttgtgat gcaagacgca gattatttaa taaagacctg
     9541 gcatccatct tgtggagcag cggcgttacg tgccgaattg attgccggat ttgcgcatac
     9601 tgaatggttg ggattgaccg tatttgaaca ttgttggcag gacgccgaca atatcggatt
     9661 tgtcagtttt gttgctcgat ttaccgaagg tggcaaaaca ggtgcaataa ttgaacgttc
     9721 gcgatttttg aaagaaaacg gtcaatggta ctatattgac ggtacacgtc cgcagtttgg
     9781 ccgcaacgat ccctgccctt gtggttcagg taaaaaattt aaaaagtgct gcggccaata
     9841 atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca atgcattcac
     9901 tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc gcacgtatta
     9961 ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt gttgatcacc
    10021 gtaccgggcg cttttttatg cgcacggaac tggaagggat ttttaatgat tccaccctgc
    10081 tggcggatct cgatagcgca ttgccagaag gctccgtgcg tgagctgaat cctgccggtc
    10141 gtcgccggat agtgattctg gtcactaaag aagcgcattg ccttggcgat ttgttgatga
    10201 aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac cacgatactt
    10261 tgcgttctct ggttgagcgt tttaatattc cgtttgagct ggtaagccat gaagggttaa
    10321 gccgcaacga gcacgatcaa aagatggcgg acgccattga tgcttatcaa cctgactacg
    10381 tggtgctggc gaaatatatg cgggtattaa cgccggaatt tgtggcgcgc ttcccgaata
    10441 aaatcatcaa tattcaccat tcattcctgc cagcgtttat cggcgcacgt ccttatcacc
    10501 aggcctatga acgtggcgtg aagattattg gcgcaacagc tcactatgtg aatgacaatc
    10561 tggacgaagg cccaatcatc atgcaggacg ttattcatgt cgatcatacc tacacagctg
    10621 aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgc gcgctctaca
    10681 aagtactggc gcagcgcgtc tttgtttacg gtaatcggac gattattctt taatcgccag
    10741 caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc agttcatttt
    10801 tctcaacgta acactttaca gcggcgcgtc atttgatatg atgcgccccg cttcccgata
    10861 agggagcagg ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg ccaaagggag
    10921 cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca
    10981 cttttcaaaa gtccctgaac tctcaaacga atccgtaatt aaatattctg ccaagcgggg
    11041 aaggatgaga agcttcgacc aaggttcgac ttgagcgcca gcgagagagc gttgccgcag
    11101 gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac caccatcgct
    11161 tcctgaaata atcaaaacct gttactccga gctaattttg cccgatggca ccacgtttac
    11221 cgggcctaca atatcctccc ctgtaggtcg gataagcgaa gcgcatccga caactcaccc
    11281 cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta aaattcgctt
    11341 agtgacgagc gcgcaccagc tggtacttac gtgtcagata ctccaccggt acgctccaga
    11401 tgtgtaccag acgcgagaac gggaacagca ggaataacgt catccccagc accagatgca
    11461 gacggaagat aaacgccaca ccatcaaggt gttgagaagc gccaccgtgg aaggtcacta
    11521 ccgactgcgc ccagccaacc agtttcatca tctcgctacc gtccatatgc tgggcggaga
    11581 acggaatggt cagcaggccc agcgcgcact ggataacgag cagtgaaagg atcaggatat
    11641 ccgctccggt agtcgttgca cgcacgcgtg ggctgaacag acgacgcttc agcaacagca
    11701 cgccgccaat cagacacagc acgccgctgg caccaccagc aaacatcgcc attttctgtt
    11761 tcacttcaat cggcagccag gcttcataca tccagtgcgg cgtcagcata ccgaagaagt
    11821 gaccgacaaa aatccccaga ataccgatat ggaacaggtt cgacgccagg ttcatccctt
    11881 tgcggtccag catctggctg gacgccgcgc gccaggtgta ctgcccgtag tcataacgca
    11941 gccagctacc aatcaggaag accgccccgg caatgtacgg gtagatatca aagaagaaca
    12001 tattcaggaa ttgcattagt gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac
    12061 ggcaccggca aagcgacgct gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc
    12121 aaagaattta acctgctctt cttcccagac agcatccagc gcctgcggcg tatcatcgcg
    12181 cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg atagcggtat tggccaattt
    12241 cagcagcaga tcaaacagca cggcataacg gctttcacgc tgttgcagac gcgcgctcag
    12301 caatgccaga atcggcgcga tatctttcaa accttccacg gcttcgcttt gcggcagctg
    12361 cgccaggtac tccagataca gcggcagatg atccggcaat tcgcggctgt ttaactgcaa
    12421 gccgtgctgc tcgtactgcg ccaacaggtc caccatcgcc tggccgcggt cgcgggattc
    12481 gccgtgcaca tgttcaaaca gcaacagtga cgtggcgcgg ccacggtcga acagttcgct
    12541 gtactgcgcc tgggcatcga gcggatccat cgtcgttaaa tcgcgcagga aaatgcccag
    12601 cgcatgggca tcctcttttg gcagattttt cgacgcggca atcgcctcaa acatctcttg
    12661 ttgatgctgc cataaggcag catccggata ttcaaggaga cgcgatacaa tcacgagttc
    12721 gatcatggat gcggctccgt tttgctggtc acatcgatgg catcgatacg acggctgttg
    12781 aacagattga atttggtatc tgaaccgtgg cagccatcac caaaggtaaa gccgcagcca
    12841 tttttctccg ggaaggcttc ccgtgccagt tcacgatgac tactcggcac cacaaagcga
    12901 tcttcgtagt tagcaatcgc cagataacgg tacatctcct gtgcctgggc ttcggtcaga
    12961 ccgacctctt ccagtgcacg ggtatcaact ttaccgtcaa cggtttcagc acgtttgtaa
    13021 tgacgcatcg ccagcatacg tttcagcgcg cgcagtaccg gtttggtatc accggcggtc
    13081 agcagattcg ccagatactg taccggaata cgcaggcttt cgacgtctgg cagaatgccg
    13141 ttgctaccca gctcacccgc gtctgctgca gactgaatcg gagacagagg cggcacgtac
    13201 cagaccatcg gcagtgtgcg atattccgga tgcagcggca gcgccagttt ccattccatt
    13261 gccattttat aaaccggcga ctgctgtgca gcttcaataa cgctcagcgg aataccgtct
    13321 ttaatcgcct gctcgatgac tttcggatcg ttcggatcga ggaacacctc cagctgacgc
    13381 tggtagagat ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc ggcatcatac
    13441 agcagcacgc caagataacg gatacgaccg acacaggttt ctgagcacac ggtcggctga
    13501 cccgcttcaa tacgcggata gcagaagatg cacttctcag acttaccgct cttccagttg
    13561 aagtagattt ttttgtacgg gcatccggtg atgcacatac gccagccacg gcatttatcc
    13621 tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc
    13681 gccacacatg ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg
    13741 ttttcgaact ggctatacat cgccttctgg atgttgtcga agttcttgtc tttcgccagc
    13801 ttgtcaaact caccgcccag gtcatcttcc cagttcggcc ctttttcgat tttcgccatc
    13861 cgttccccgg taatcagcga acgcggacgg gcaatcggct gcgatttgct gccttccggc
    13921 gcggtatgca ggttctgata gtcaaaatcg aacggctcgt aataatcgtc gatccccggc
    13981 agatgcgggt tagcgaagat tttacccagc agcatggcac ggttacccat gcgcggttgc
    14041 agtttgccgt tgattttacg gatccagccg cctttgtatt tttcctggtt ttcccagtca
    14101 gtcgggaagc cctggcccgg cttggtttcc acgttgttga accacgcgta ttccacgcct
    14161 tcacggctgg tccagacgtt tttacaggta actgaacagg tgtggcagcc gatgcactta
    14221 tcgagattca gcaccatgcc gacttgtgaa cgaattttca ttttacgctc tcctgtacct
    14281 ggtcattgcc ttcgccatct aaccagtcaa tgttcttcat cttacgcact acaacaaact
    14341 catcgcggtt agaacctacg gtgccgtagt agttaaagcc gtatgccaga tgggcatagc
    14401 cgccgatcat atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg ataccaccac
    14461 gctgttgggt aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg tggtacatca
    14521 tggtcatccc tgccggaaca cgctggctga caaccgcacg ggcagtcagc gcaccgttgc
    14581 tgttgaagac ttcaatccag tcgttatcgg cgatacccag atctttggca tcggcttcac
    14641 tcaaccagac caccggacca ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg
    14701 tggagtggat accccacttc tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc
    14761 cgttggattt ctggcctatc acttctttca ccgaacgggt gtcgatcggc ggacgataaa
    14821 ccagcaggct ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt tgctgacgac
    14881 cagagagcgt acgccatggg atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa
    14941 cgtgttcatc ttccagacca gaccaggtcg ggctggagat aattttgcgc ggctgtgcct
    15001 gaatatcgcg gaagcggatc ttctcgtctt ctttattcag cgccagatgc gtatggtcac
    15061 gaccggtaaa ctcgctcagg gcagcccagg ctttcacggc tacctgaccg ttggtttccg
    15121 gtgccagtgt caggatcatc tctgccgcat caattgcggt gttcagcatc ggctggcctt
    15181 tcgccggacc ttccgctttg gtgtagttga gcttacgcag cagatccatc tcgctctggg
    15241 tgttccaggc aatcccttta ccgccgttac cgattttctc catcagcggg ccgatagagg
    15301 taaagcgttc gtaagttgcc ggataatcgc gctctacgac cataatgtgc ggtgcagttt
    15361 tgcctgggat caggtcgcat tcgccttttt tccagtcttt cacatccagc ggctgcgcca
    15421 gttcagcggc agagtcgtgc tggataggca gcgtgacgat gtcggtttct ttacccagat
    15481 ggccgacgca cacttcggag aatttcttcg cgatggcttt gtagatttcc cagtcgcttt
    15541 tcgcttccca ggccggatcg accgccgcag acagcgggtg aataaacgga tgcatatccg
    15601 aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga
    15661 gacaggtgct cgacagacgg aagtccagcg taaccaccag atccagcttg ccttccagac
    15721 cattgtcctg ccagtccact tcttccggct tcacgccgcc ctgctgcccc agatctttac
    15781 cctggatacc gtgctccgtc cccagcaggt acttgagcat aaactcatga cctttaccgg
    15841 aagaaccgag caggttagaa cgccagatga acaggttgcg cgggtggttt ttaccgtttt
    15901 ctggttgttc tgccgcaaaa cggatggaac cctctttcag ggatttcacc gtatagtcca
    15961 ccggattcat cccggctttt tccgcttcgc cagcgatagt cagcgggtta gtgcctaact
    16021 gcggtgcaga cggcagccag cccatgcgtt ccgcacggac gttaaagtcg atcaggtgtc
    16081 cggtataacg ggatttatcc gccatcggtg acagcaactc ttccgccgtg acggtttcat
    16141 aacgccactg gctggagtgg ttatagaaat aagaagtgct gttcatgtga cgcgccggac
    16201 gctgccagtc aagggcaaac gccagcggct gccagccggt ttgcggacgc agtttttcct
    16261 gacctacata gtgcgcccag ccgcccccgc tctgaccgac acagccgcag aaaatcagca
    16321 tgttgatcag accacgatag ttcatatcga ggtgatacca gtggttcagc cccgcaccga
    16381 cgataatcat cgaacgaccg tgcgttttat cagcgttatc ggcaaattca cgggcgatgc
    16441 gaataatctg gctgcgagaa acgccggtaa tttgctcggc ccaggccggg gtataagctt
    16501 tcacatcgtc atagctggtt gcacagttaa cgtcgttcag gccacgttcc agaccgtagt
    16561 ttgccagcgt cagatcataa acggtggtca ccagggcggt gctgccatca gccagttgca
    16621 ggcgtttcac cggcagtttg tgcagcagca cgttttccag ttccactttg ttgaagtgtt
    16681 ccgtgccgtc gccaccaaag tacgggaagc ctacctcagc gatctcatcc tggctaccca
    16741 gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc
    16801 atttgccctt ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc atttcgccat
    16861 tggtattaaa ggcgacagtt ttccattccg gattgttttc ctggcccagc gcatcaacca
    16921 gatcagcagc gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct tccagcatca
    16981 ccagcatcgg catgtcggtg tagcgacgaa catagtcggt gaaatactgg cttgggttgt
    17041 cgaggtggaa ttcacgcagc attacgtggc ccatcgccag cgccattgcc gcatcggtgc
    17101 cctgtttcgg tgccagccac agatcgcaca gtttggcgat ttcagcgtag tctggtgtga
    17161 cggcaacagt tttggtgcct ttgtaacgca cttcagtaaa gaagtgagca tccggggtac
    17221 gcgtctgcgg cacgtttgaa ccccaggcga tgatgtagct ggagttgtac cagtcagcag
    17281 attccggtac gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc aggtcgcagt
    17341 accagtcgta gaagcttaag caagtaccgc caatcagcga gagatagcgt gcacccgatg
    17401 cgtaagaaac catcgacatt gccggaattg gcgagaaacc agcaacacgg tccgggccgt
    17461 agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc gttcacttcc tgccaggaag
    17521 aacgaacaaa accaccgcgt ccacgcgcct gcttaaagct tttcgcttta ttggcgtctt
    17581 caatgataga agcccatgcc tcaaccggat cgctatgcag cgccttcgct tcacgccaca
    17641 ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg gttggcgctg taaagatacc
    17701 aggagtagct ggcaccacgc gggcagccgc gaggttcatg gtttggcagg tccggacggg
    17761 tacgcggata gtcagtctgc tgggtttccc aggtgaccag accgtttttg acgtagattt
    17821 tccagctgca ggagccggtg cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct
    17881 gccaacgctg gcgatatcca tcctcccagt cacggttggt attgagaagc tggccatgcc
    17941 catcggcaaa ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg aatttactca
    18001 tcggttttct cctgtgggag cctgtcggcc ctctgataaa tcgacattgc taatgtttaa
    18061 gggcaaggta acgctctgaa acagcgtggg aattgataac gatcaagagt gatggggatg
    18121 aaaaataaag taaattccta agatacccct ttaggataat taacagttac agcataacat
    18181 gctgtaaatt aaggcgtttt atacctgtag ttatttaaac t
//
LOCUS       DAPGRN010000099        17561 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPGRN010000099 DAPGRN010000000
VERSION     DAPGRN010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.100"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            184..3756
                     /locus_tag="RHW33_003517"
     CDS             184..3756
                     /locus_tag="RHW33_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV1646986.1"
                     /translation="MNGSDPVTEFAQVLENAGLVLKELPAMDGKIHRVPTTDDKNGQK
                     SGAYRGFLDGRPAGWYRDYRSADDSPVNWTFSGGEQTDPRARLHLKAHSLQRREDAER
                     ELKAQYNRQAAYARRYVNKWPQATAHEYLTRKGIQAAPGVRINDKNELVIPFRNRNGA
                     IRSYQRIPVTGGKDARILKDSEKTGNWFALGTPRNGQPVLFAEGYATAASLHEATGLP
                     VLMTVDAGNMIAVAEDARQKWKQSPFIFCADNDHARQVNKGIVSATKAAELTGGSVIF
                     PAFTDAEKAQGLTDFNDLDASRGRAAFQHVINAQLEHIGVSTPNSNTPEIREALVIGN
                     LVFTPVHTEEKTMTPTEYPETSPDTGHSHDQGPSLPSATQQEQPTASSSNIADETLSF
                     TSHATENNGKDERHPDNVQAQTESSVVQDSPAESPASPESTTASAPDEPAHPAEPPEK
                     VVSVATDKDWREFEAELSQPEKNELQQESGTSPEIPAPAAAPAAPEDSPSSETMNESA
                     EPSPSPDAVKEEMTVMTEPDDKQSTGISPDTPREEAVSHVTEDIPLQPEPFLPESDGP
                     EEYEDYSAYQELMNNENPEHLQENSDMPSQPDTGPVQADESELQATTATIEPAVRDEP
                     TPQQPVQSTSPSDNTSSFLDKARGLFTRKKTDSQAHENSDPTPSAETTTTPPIPDSIV
                     YAPERSDAPISLNMDEILKELDWEKRADRTVLYKLDGKPAFIDRVNRLEMVNGASNDD
                     RSVLAALAVATKFYGGVIELTGSDAFKQKAMQLIIEHNIDVRMKLPDQRAELEKLRKE
                     MAVSKDAVVTHQPTPELNRNTPEQPAVPDPVQEKEATQSPASPVAPEASTVSTASAPS
                     PAEPGKTADVAPDEEPPGKLRPGESVTAVLHNFGRAEYAPGKGESFFVELKNRSGSKL
                     YWGEQLESLVKNHQKGDVVTLTLQNREQFILPGEQKARFRNKWSMESVTNGISVSHDN
                     PDKGQRIQAIPVETFMKVAAQISQGWPEEMKALRMPENVGSHLFIGEDRHPVSAPQNA
                     NQVTEITSAAPDKLTPVLGSVDKDTRELNLLLVQSADEHLQGVVRLNGTLYPALATPS
                     ADNSQLVINALTDKGLRFAGYGEAVNHDADSTNRPAPELMQFHLKTREEPLFAAVYTP
                     EKQPDALYRNLGFEQSWQQWSNSQKPEDRQEKTLHQDLSHSPGR"
     gene            3774..4121
                     /locus_tag="RHW33_003518"
     CDS             3774..4121
                     /locus_tag="RHW33_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001629514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1646987.1"
                     /translation="MNMHKSLVAVLGLILISSTASAATCRAGSAAQAGSNTGYERARA
                     AADAWSQRENDVSSSLQSCLSRIKKISINLPQFPSLDDILSQLENQVCDAVVDKVNEK
                     LPGNIDPWKDYNL"
     gene            4118..4810
                     /locus_tag="RHW33_003519"
     CDS             4118..4810
                     /locus_tag="RHW33_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HDV1646988.1"
                     /translation="MTENTEPSSPSHPAPDPEILKSTIRAMKQSEQRANNVPALIKAL
                     LCTGTCLLISITGNAIQYWHSTNVEREYFATDNGRLVRLAPTSQPAWSQNDAMAFGSQ
                     ALATAFNLDFVHYRSQISSLSPRFSDEGFAGYVNALQASNILETIKKEKMNLTATTGA
                     GVLVRQGQMSDGVWFWTFQYPVRMRLVGQTTSKPEQSFVFEITIQRVDPRLKPSGMEI
                     RQMISRNAGPNS"
     gene            4821..5804
                     /gene="traN"
                     /locus_tag="RHW33_003520"
     CDS             4821..5804
                     /gene="traN"
                     /locus_tag="RHW33_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraN"
                     /protein_id="HDV1646989.1"
                     /translation="MQSRYLLSTLLLVCSAATSADNAGWQNARTPQTTNSASDHVQNA
                     SQNTGGVPATLVKGELPAPGQASPLVQDAARLDSELSADEIRSLRSLMADNERAINAP
                     ITSVVPRISSLTVNLSPGASLPLVRTAMNNLSVVTFTDINGSPWPQSDPPYNAAPKLF
                     DVQYNENMVTITPLRPWASGNISVYLKGLSVPVILNVTSGETDTPSSSQEMDSRLDLR
                     IPRQGPTSPVVSIPTDKIALHDATLQAFLDGIPPRDPSVKRLKFTGNVPDTTIWQHGD
                     DLLVRSRAMLRDEFEQTLSSADGTHLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            5807..7096
                     /gene="traO"
                     /locus_tag="RHW33_003521"
     CDS             5807..7096
                     /gene="traO"
                     /locus_tag="RHW33_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HDV1646990.1"
                     /translation="MSAEQDAGKSGKKLAALLGLGSIILFGVGYIAFSKISGSNSDMQ
                     SAVNINSAASGGTRSVTETPHYRELLRADNERGAAAAARNNQTFIASLPQGLDIPDTP
                     EKQQQPAAKPENYAHRQASGTPQEDRAASEKRMERLQKLIVRIKDQHPAGSTPTIATA
                     MWNKSPAETTGQNGTQQFALQNASLSTPVAEKGIQLIPALTRIPAYIDTAVDSDNPSS
                     KVIATIPAGPWAGATLFSPGVKLVGNGVEIHFDRMSWNGMDLKVNAYAQREDNLMSSV
                     ASNVNTRWFKHIILPSVLGGVGSIGTLYKDANTQVIQGNYGTVTGRVGMPSGEAVAGV
                     IAGGMAERGSQILTRQAESEPYKQVEVYQHEVVSILFVDPVMTNDARSSSLSSGISPS
                     VNRTSQTEQRSQARMQTAMEQRKAVMQRRYDEQPETP"
     gene            7096..7800
                     /gene="traP"
                     /locus_tag="RHW33_003522"
     CDS             7096..7800
                     /gene="traP"
                     /locus_tag="RHW33_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraP"
                     /protein_id="HDV1646991.1"
                     /translation="MKPETEIDETGSFGEPEEKPAPFWKRSVWGISVATWGLCAVVLL
                     AAIWYLFLRAPSETGMPPFNDADAGVQTWQTTQESSPSVQSTETMTVRTGEDMSQLAR
                     DVKTELDNRDEKIQATLNMLHDSINKLGEAIKKDEEYAQETRRQLDDIRSHLNGMMTQ
                     KSVTESSSTPHPAKKKTSSVLNGMKIMSMETGMAWIRWQGSTWAVREGQTLGNVVIQR
                     IDPTTRTIITSAGTLR"
     gene            7800..8327
                     /gene="traQ"
                     /locus_tag="RHW33_003523"
     CDS             7800..8327
                     /gene="traQ"
                     /locus_tag="RHW33_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HDV1646992.1"
                     /translation="MNMDALTAIENFASSIFSAGMDFLFTWGEFIGVISMITLFARAR
                     SAGPVKMSPGKFIAGMLTSCMLVSLPAMINAGGVQMGFRADSFGPIAYVQPQTFGAAA
                     GAANAVLSLAKLAGVGFVMNGISIWRKAGLDGHTALSASESVSKGNVKFIAGVLLVFI
                     DRVLNALLASIGIVF"
     gene            8378..8782
                     /gene="traR"
                     /locus_tag="RHW33_003524"
     CDS             8378..8782
                     /gene="traR"
                     /locus_tag="RHW33_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraR"
                     /protein_id="HDV1646993.1"
                     /translation="MSRIRVVAARLYAAFIHAQLFVMSCRSKLTKFLLLIPMMLLPKA
                     VLADGDLADMVRNVEQGAKTAQSSSLTIAQFIGVILFLGGLIGLKKVGKQGGMGLAGC
                     IVSIVIGALLVAGPEMMSRSQKQLGISTISIG"
     gene            8846..9034
                     /gene="traS"
                     /locus_tag="RHW33_003525"
     CDS             8846..9034
                     /gene="traS"
                     /locus_tag="RHW33_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative conjugal transfer protein TraS"
                     /protein_id="HDV1646994.1"
                     /translation="MSAWSPEHITLRRTENGNVIAHDSRDDQEFIFAECCSDEIANII
                     LEAVKRYTSTEPAHATQH"
     gene            9018..9818
                     /gene="traT"
                     /locus_tag="RHW33_003526"
     CDS             9018..9818
                     /gene="traT"
                     /locus_tag="RHW33_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraT"
                     /protein_id="HDV1646995.1"
                     /translation="MQHSIKDLWLYPFPEIDVVHTQEPLLPEPELTTPGRCICCRQNV
                     RHRFRLDDSWPLRQLTDTISDTRVRLNKATEHLDKLKKRGEPVATGEKEKYNTAVKAA
                     ERALEQARLSARRLSLRHVQKAEITSTESLSEKEQELFHEDGPPYSLCAFCHAWHSLN
                     GYAAAQGVMVWLPDLHPSTVVALNRRSLQEVFSNDKFRVRRGREALSALMQNRLAVED
                     KFRSFRPADFADVFRRYPPSGRSPLREKMNGIALILTPDSFIKKEYVD"
     gene            9908..12952
                     /gene="traU"
                     /locus_tag="RHW33_003527"
     CDS             9908..12952
                     /gene="traU"
                     /locus_tag="RHW33_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HDV1646996.1"
                     /translation="MNINRLIFTIEDCLNTLSRFSVASSFVEYCDLRTVIGLDRQDRE
                     RRPWLNSPYIAATKRGEYLSVFEVSGAFREMDEASDQTGPGSLESLITSMSDSLNTAY
                     KNSGHKISFVFERDPDMGKEEIEDMVAPQKRSLANTGIQLQDVVDEKVTTLSPWLVRE
                     RCWLAIWSGPDLISNSDRTAHDELVRRLAERVPKARFAQSPWQWTLSALKIRHEAFLD
                     NVEQALRHSSDGLILRLLDIHEVGREIRRQTERHSTPRNWQPHLPEDAQPAGYRWTDD
                     ESVLHAPSLHLQLFNTQVTTQGNLVQAGGLWHGMVSITLPPQNLQTFNELVRAVPRAV
                     PWRIRMDLMPGGMKALNLKKTLLTYSSFISAVRPMYESVMTLAATDEKEPVCIMTIMA
                     STWGKTREICTRNQAILKSAIEGWGVCGTTTTFGDPRRAWVNTILAASGGSGPVPLYP
                     PLSHAISLFPLNRAGSVWRGKGNLMLHTEDGSAFEVGLASSQQNKHTELAPGDPGLGK
                     SVLINTLSEIQISSAQKNLPFIAYIDKGYSAQGLVQLIRDSLPPERKDEAVGIILSND
                     PEYTRNLFDVMYGAKKPITPEKNFMSSVLCALCVDTGTGQPCNPGDTRQIINQLIELA
                     FKEYGENNPRLYRASTEELVDSALQDSGLYEKHDATWWARSTWFEVRDMLHNAGYIMA
                     AQRAHYQAMPQLPEVSSMLGHTSLRDVFGTVQRDGSNELLLDYIRRALEQGHNDYPMI
                     SGYTRFMINPETRVIAVDLNNVAGDKTPAGRLKTGIMYLLAGQIAGGDFTLPQYRDEV
                     LKQLPREYHEIALKRINQLDQEVKTKVYDELHNARGIDFIWENLDTQEREQRKFAIRT
                     VLSTQYLRDYPESVLKSANTLWLLRYKPEDIPVLRDNFNVPEFMLKRFLKMPEGPAPD
                     GSGVPVLGVFRVKSGTLARILKFTVGPLELWALNSSPKDSALRKTLTNKLGSVRARKI
                     LAENFPRGSATSLIEHRAGQHNSDNVIEELASELIRKQGYNL"
     gene            12952..13566
                     /gene="traV"
                     /locus_tag="RHW33_003528"
     CDS             12952..13566
                     /gene="traV"
                     /locus_tag="RHW33_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HDV1646997.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRYQCFPLAPLPEDRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            13533..14735
                     /gene="traW"
                     /locus_tag="RHW33_003529"
     CDS             13533..14735
                     /gene="traW"
                     /locus_tag="RHW33_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HDV1646998.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANAR"
     gene            14764..15348
                     /gene="traX"
                     /locus_tag="RHW33_003530"
     CDS             14764..15348
                     /gene="traX"
                     /locus_tag="RHW33_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HDV1646999.1"
                     /translation="MNKLPENSDQTKNVKKSPSITRIATRGIINIFVPLMETRLITNS
                     VQHMLEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFRRRRTVWRFCC
                     WSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALIVTYRLWQLHE
                     RKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
     gene            15445..>17561
                     /locus_tag="RHW33_003531"
     CDS             15445..>17561
                     /locus_tag="RHW33_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HDV1647000.1"
                     /translation="MKILLRALCAGLAISSLPAMASVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLSTSAPTLIGSMFGAFNSIIAVLAVVWFMFIGIRHVVRSGHQGQVFST
                     GRDIVGTLSVVAGFLMIVPTGNGWSIAQLIMLWGASIMGVGSANVMVQLAADNIANGY
                     SMTVQPVQASTRTAARGIFEMELCKYAVNAGLNDFNQTVKSSTSLMTESAKTASGNYT
                     VTVSNGSGICGSASLSVEGNGTTDQSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDM
                     DNAASEFVTTFLEKRNSGNGTLPDIETRIQRAADEYERAVQKSLPIDNGEQSRKEALK
                     SYLTTYGWVTLGAWYQTFATANQRLAELADRAPAVTSMSSLGEVGDTDLFSAVMGAYK
                     TQLQNSTYTPTIGTITTQDESNAANSTDPQSVVMKSIAPTILKWTNQAATEWSGTGTT
                     SNQVNPLIKMKNIGDYTLGTTEILWTGYTTVRVLATMGDNSIFGKAVNLFSGLPKGFV
                     ALLDAAAPPIYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWA
                     ATHLGTSEDRGSRAAYGYIYLIDSMIRPPLMVFGFFFASVAIIAVGTILNSLFGAALV
                     NVQVKSLTGIFSLAGFLLIYARMCTTSVAAIFALQAYLPDHVINFLGGRDGVNTLGNL
                     TSSVKDIFAGSNRNIRHSPGVRE"
BASE COUNT         4716 a         4620 c         4386 g         3839 t
ORIGIN      
        1 tcccgctacc gcacctggct tgccgtgcct gcagatgaaa tagaagatct caaaaaagca
       61 catcccccga tgaacgggca cacgcccgtc ctctgggata aagagcacaa actctggttt
      121 gcccgcccgg gggcggacct gtccatgctg gaccgctggc tgccccgacc gcaggaagtc
      181 tccatgaacg gcagtgatcc ggtcaccgag tttgcccagg tactggaaaa tgccggactg
      241 gttctgaaag aactgccggc catggacggt aaaatccacc gggttccgac caccgatgac
      301 aaaaacgggc aaaaaagtgg cgcatatcgc ggcttcctgg acggcagacc tgccggctgg
      361 tacagggatt atcgcagcgc ggatgattcg ccggtcaact ggaccttctc cggcggagag
      421 cagacagatc cccgcgcaag gcttcacctg aaagcgcatt cgctgcaacg cagggaggac
      481 gctgagcggg aactgaaagc acagtacaac cgacaggctg cttatgcccg ccgctatgtc
      541 aacaaatggc cgcaggccac ggcacatgaa tacctgaccc ggaaaggcat tcaggctgcg
      601 ccgggggtgc gaataaacga caaaaatgag ctcgttattc ctttccgcaa ccgtaacggc
      661 gcaatacgct cctaccagcg cattccggtc accggcggga aggatgcccg catcctgaaa
      721 gattccgaga aaaccggtaa ctggtttgcg ctgggtacac cgcgcaacgg ccagccggtg
      781 ctgtttgccg agggctatgc cactgccgcc tccctgcacg aagccaccgg ccttccggtg
      841 ctgatgacag tggatgcggg caacatgatt gccgtggccg aagatgcccg gcaaaaatgg
      901 aagcaaagcc cctttatctt ctgtgccgac aacgatcatg cacgccaggt taacaaaggt
      961 atcgtcagtg caacaaaagc cgcagaactg acaggaggat cggtcatctt tcctgcgttc
     1021 accgatgccg aaaaagcgca gggactgacc gatttcaatg acctggacgc cagccgggga
     1081 cgggccgctt ttcagcatgt catcaacgcc cagttagaac atatcggggt ttccaccccc
     1141 aacagtaaca ccccggaaat ccgggaggca ctggtgatcg gcaacctcgt ttttacaccg
     1201 gtccacactg aggaaaaaac catgactccg acagaatatc ctgaaacatc acccgataca
     1261 gggcacagcc atgaccaggg accttctctg ccgtccgcca cgcagcagga acaaccaact
     1321 gcttccagca gtaacattgc tgatgaaaca ctgtcattta ccagtcacgc cactgaaaat
     1381 aacggcaaag atgagcggca ccctgataac gttcaggcgc aaacggaaag ctctgtagtt
     1441 caggactccc ctgcagaatc accggcatcc ccggaaagta caacggcgtc tgcccctgat
     1501 gaacccgctc atccggcgga acctccggaa aaagttgtct ctgtggccac agataaagac
     1561 tggcgtgaat ttgaagccga actgtcacaa ccggaaaaaa acgagttaca acaggagtcc
     1621 ggcacttcac cagaaatacc ggcccctgct gctgcccccg ccgcccctga agacagcccc
     1681 tcatctgaaa ccatgaatga atcagccgaa ccatcacctt cccctgatgc agtcaaagag
     1741 gaaatgaccg tgatgacgga gcctgatgac aaacaaagca ccggaatatc cccggatact
     1801 ccccgggaag aggcggtcag ccatgtaact gaagacattc cccttcagcc agagccgttc
     1861 cttcctgagt cagatggccc tgaagaatac gaagactaca gcgcatatca ggagctgatg
     1921 aacaatgaaa atcctgagca tctgcaggaa aacagtgata tgccgtcgca accggacaca
     1981 ggccccgttc aggcagatga gtcagaactt caggccacaa cagccactat agaaccggct
     2041 gtccgggatg aacccactcc gcagcaacct gttcagagca catcaccttc tgataatact
     2101 tcatcgtttc tcgacaaagc gcgtggcctt tttacgcgta aaaaaacgga ttcacaggcg
     2161 catgagaaca gtgatcccac gccctcagcg gaaaccacga cgactccacc gatacctgac
     2221 agtattgtct acgccccgga acgttctgat gcaccgatat cgctcaacat ggacgaaatt
     2281 cttaaagagc ttgactggga aaaacgagca gacagaaccg tcctgtacaa actggatggc
     2341 aaacctgcct tcattgaccg ggtaaaccgg ctggaaatgg tcaacggtgc cagcaatgac
     2401 gatcgcagcg ttctggccgc acttgctgtg gccaccaaat tttatggggg cgtcatagag
     2461 ctgacgggca gtgatgcctt taagcaaaaa gcaatgcagt tgattatcga gcacaacatc
     2521 gatgtccgga tgaaattgcc agatcaacgg gctgaactag aaaaattacg caaggaaatg
     2581 gctgtcagta aggatgcagt tgtcacccat cagccaacac cggaacttaa ccggaatacc
     2641 ccagaacaac cggctgtccc tgaccccgtt caggaaaaag aagccacaca atccccggcg
     2701 tccccagtag cccctgaagc atcaaccgtc agcaccgcct ctgcaccatc accggctgaa
     2761 ccaggcaaaa cggcagatgt ggcaccagac gaagaaccac caggaaaact tcgcccggga
     2821 gagagcgtga ctgccgtact gcacaatttt ggtcgtgctg aatatgcacc cggcaaagga
     2881 gagagtttct ttgttgagct taaaaaccgc agtggcagca aactctactg gggggaacaa
     2941 ctggaaagtc tggtcaaaaa ccatcagaaa ggtgacgtgg tgacactgac acttcagaac
     3001 cgcgaacaat tcatcctccc gggagagcaa aaagcacgtt tccggaataa gtggtccatg
     3061 gagtcagtta ccaacgggat ctcggtatca catgataatc cggataaagg tcagcgtatt
     3121 caggcgattc cggtcgaaac ctttatgaaa gtggctgcgc aaatcagcca ggggtggccc
     3181 gaagagatga aagcgttacg tatgccagaa aacgtcggct cacatctgtt tatcggtgaa
     3241 gaccgacatc cggtctccgc gccacagaac gcaaatcagg taacagaaat aaccagcgca
     3301 gcccctgaca agctgacacc tgttctggga tcagtggata aggatacgcg tgaactcaac
     3361 ctgctacttg tccagagtgc cgatgaacat ctgcagggtg tggtccggct gaacggcaca
     3421 ttgtatccgg cactggccac cccatcagcc gacaacagtc agctggttat caatgcgctg
     3481 accgacaaag gtttacgctt tgcaggttat ggtgaagcgg taaaccacga tgctgacagc
     3541 acgaatcgcc ctgcaccgga gcttatgcag tttcatctga aaacccggga agaaccgctt
     3601 tttgctgctg tctacacgcc ggaaaaacaa cctgatgcgt tgtaccggaa cctgggcttc
     3661 gagcagtcat ggcagcagtg gtccaacagc caaaagccgg aggacaggca ggaaaaaact
     3721 ctgcatcagg atctctccca cagccccggc cgatgagccg gtaaggaagc catatgaaca
     3781 tgcataagtc tctggttgcc gtgctcggcc ttatcctgat ttcatccacg gcaagtgctg
     3841 caacctgtcg tgcgggtagc gcggcacagg ccggtagtaa tacaggatat gagcgagcac
     3901 gtgcagctgc ggatgcatgg tcacaacgtg aaaacgatgt ttcctcatcc ctgcagtcct
     3961 gtctgtcacg aatcaaaaaa atcagcataa acctccccca gttccccagc cttgacgaca
     4021 ttctgtcgca gctggaaaac caggtctgcg acgcggtcgt ggacaaggtg aatgaaaagc
     4081 tgccggggaa tattgatccc tggaaggact acaacctatg accgaaaata ccgagccatc
     4141 ctcgccatca catccggcac ctgacccgga aatactgaag agcacaatca gggctatgaa
     4201 gcagtcagaa cagcgggcaa ataatgtccc tgcgctgata aaagcgcttt tatgtacggg
     4261 cacatgtctt cttatcagta ttactggtaa cgccattcag tactggcact caacgaatgt
     4321 ggagcgcgaa tatttcgcta cagacaatgg tcgtctggtc aggctggcac caacaagtca
     4381 gccagcatgg agtcagaatg atgcaatggc ctttggcagc caggcactgg cgaccgcatt
     4441 caacctggat tttgtgcact atcgttcaca gatctcctct ctgtctccgc gtttttctga
     4501 cgaaggtttt gccggatatg tgaatgcatt acaggcatct aacattctgg agacgatcaa
     4561 gaaagagaag atgaatctga ccgccacgac aggcgctggc gttctggttc gacagggaca
     4621 aatgtccgac ggcgtatggt tctggacatt tcagtatcct gtccgtatgc gtctggtcgg
     4681 ccagacaacc agcaagccag aacagtcttt tgttttcgaa atcaccatcc agcgtgttga
     4741 ccccagactg aaaccatccg gtatggaaat ccggcagatg atctcccgta acgccggacc
     4801 gaactcctga gataagtaat atgcaatcac gttatttact gagcacactg ctcctggtgt
     4861 gcagcgccgc cacgtctgca gataacgcag gatggcagaa cgcacgcaca ccacaaacga
     4921 caaattctgc cagcgatcac gttcagaacg cctcccaaaa caccggaggt gttccggcaa
     4981 cactggtaaa aggtgagctt cctgcccccg ggcaggccag cccgctggta caggatgcgg
     5041 caagacttga ttctgagctc tccgccgatg agattcgctc actacgcagt ctcatggcgg
     5101 ataacgagcg ggccatcaat gcgcccatca ccagtgttgt accccgtatc agctctctga
     5161 cagttaatct gtcccccggt gccagccttc ctcttgttcg cacagccatg aacaacctga
     5221 gcgtagtgac atttaccgat atcaacggtt caccatggcc acaatccgat cctccttata
     5281 acgcggcacc aaaactcttt gatgtgcagt acaacgagaa catggtgaca atcacaccat
     5341 tgcggccatg ggcatccgga aacatttctg tctacctgaa aggtctgtcg gtaccggtca
     5401 tcctgaacgt aaccagtggc gaaacagata cgccgtcatc cagtcaggaa atggacagtc
     5461 gtctggatct gcgaatacca cggcagggac caaccagccc ggttgtctcc atcccgacgg
     5521 ataaaatcgc cctgcatgat gcaacattac aggcatttct ggatggtatt cctccccggg
     5581 acccttcagt taagaggctg aaattcacag gtaatgtccc ggatacaacg atctggcaac
     5641 acggcgatga cctactggtt cgttcccggg cgatgttgcg cgatgaattt gaacagacct
     5701 tgtcctctgc cgacggaact catctctgga aacttcccgt caccccacta ctgactttct
     5761 ctgtgaatgg ccagtccgtt cacgtgacac cagaactgga gtaatcatga gtgctgaaca
     5821 ggatgccggt aaatccggaa agaaactggc tgccctgctt ggactgggca gcatcatctt
     5881 atttggcgtc ggctatatcg cgttttccaa aatatcaggg agtaacagcg atatgcagtc
     5941 agccgtgaac attaacagtg cagcaagcgg cggaacgcgc agcgttaccg aaacgcctca
     6001 ctatcgtgaa ctgttaagag ctgataatga acgaggggcc gcagctgcag cccgcaataa
     6061 tcagacgttc attgccagtc ttccacaggg gcttgatatt ccggacactc cggaaaagca
     6121 acaacagcct gcagcaaaac cggaaaacta tgctcatcgc caggcttccg gcacaccaca
     6181 ggaggatcga gcagccagtg aaaaacgtat ggagcggctg caaaagctga ttgtaagaat
     6241 aaaagatcag catcctgccg gtagtacacc aaccatagca acggctatgt ggaataaatc
     6301 tccggcagaa acaacagggc agaatggtac gcagcaattc gcattacaga atgccagcct
     6361 gtccacgccg gtagcagaaa aagggataca actgatacct gctctgaccc gtataccagc
     6421 atatattgat acggcagtgg attctgataa cccgtcatcc aaggttatcg caaccattcc
     6481 cgctggcccc tgggcagggg caacgctgtt cagtccggga gtcaaactgg tgggtaacgg
     6541 cgtggagatc catttcgacc ggatgagctg gaacggcatg gaccttaagg tcaatgcata
     6601 tgctcaacgg gaagacaacc tgatgtcatc cgtggccagc aatgtgaata cacgctggtt
     6661 taaacatatc attctgccgt cagtgttagg aggtgtgggc agtattggca cactttataa
     6721 agatgccaac acgcaggtta ttcagggtaa ctatggcacg gttaccggac gcgtgggaat
     6781 gcccagtggt gaagctgttg cgggcgttat tgccggtggt atggcggaaa gaggaagtca
     6841 gatattaacc cgccaggcag aaagtgagcc ctataaacag gtagaagtgt atcagcatga
     6901 agtcgtatca attctgtttg ttgacccggt aatgacaaac gacgccaggt catcttccct
     6961 ttccagtggc atttcccctt cagttaatcg tacatcacag acggaacaac gctcgcaggc
     7021 acgaatgcag acagccatgg agcaaagaaa agctgtgatg caacgtcggt atgatgaaca
     7081 accggagaca ccataatgaa acctgaaaca gaaatagatg aaaccggcag cttcggtgaa
     7141 cccgaagaga aacccgcccc tttctggaaa cgttccgtct gggggatatc tgtagcaaca
     7201 tgggggctat gtgcagtcgt attactcgca gccatatggt atctgttcct gagagcacca
     7261 tcggaaacag gcatgcctcc gtttaatgat gcggatgctg gcgtacagac ctggcaaacc
     7321 acacaagaat catcaccttc cgttcagtcc actgagacca tgactgtccg gacaggagaa
     7381 gatatgagcc agcttgcccg ggatgtaaaa acagagctgg ataaccgcga tgaaaaaatt
     7441 caggcaacac tgaatatgct tcacgacagc attaacaagc tgggcgaagc tataaaaaag
     7501 gatgaagaat acgcacagga aacgcgccgt cagctggatg atatccggag ccacctgaac
     7561 ggaatgatga cacagaaaag cgtcaccgaa tcctcttcca caccgcatcc agcaaagaaa
     7621 aaaacgtcgt ctgtacttaa cggaatgaaa atcatgtcga tggaaactgg catggcctgg
     7681 attcgctggc agggtagtac ctgggctgtc agagaaggtc agactctggg caatgtggtg
     7741 atccagcgga ttgatccgac gacccggacc atcatcacca gtgcagggac gttacgctga
     7801 tgaacatgga tgccctgacc gccattgaga actttgccag cagtattttc agtgccggaa
     7861 tggatttcct gtttacctgg ggagaattca tcggggtgat cagcatgatt accctgtttg
     7921 cccgcgcccg ttcagccggt ccggtaaaaa tgtctcccgg aaaatttatt gcggggatgt
     7981 taaccagctg catgctcgta tcactcccgg caatgattaa cgccggaggc gtacaaatgg
     8041 gattcagggc tgactcattt ggcccgattg catatgtaca acctcagacg tttggcgctg
     8101 ctgctggcgc agcaaatgca gttctgtcgc tggccaagct ggccggtgtt ggatttgtaa
     8161 tgaacgggat atccatctgg cgcaaggccg gactcgatgg acataccgca ctgagtgcca
     8221 gtgaatccgt cagtaaggga aacgtcaaat ttattgccgg cgtcctgctg gtctttatcg
     8281 atcgtgtcct caatgccctg ctggcaagca ttggaatcgt cttctgattt ttttcacctt
     8341 catataaacc actgatatat ccatttaagg agaaactatg tcacgtattc gtgtcgtagc
     8401 ggcccgtctg tacgccgcat ttatccacgc acaactgttt gtaatgtcat gccggagcaa
     8461 actgactaag ttcctgctgc tcattcccat gatgttgctg cccaaagccg ttcttgccga
     8521 tggagatctt gccgatatgg ttcgcaatgt cgaacaaggt gccaaaacag cacagtcatc
     8581 atcactgact attgcgcagt tcatcggcgt aattctgttt ctcgggggcc tgattggcct
     8641 gaaaaaagtt gggaaacaag gggggatggg actggctggt tgcattgtat ctatcgtcat
     8701 tggggcgcta cttgtcgccg gacctgaaat gatgagtcgc agccagaaac agctggggat
     8761 ttccacaatt tccattggct aatcttgtcc agggcactgg catttatccg gtgcccttcc
     8821 tgttcggcaa tacaaggagg cacatatgtc tgcctggagc ccggagcaca taacgttacg
     8881 acgaacagaa aacggcaatg tgattgccca cgacagtcgg gacgatcagg aattcatttt
     8941 tgcagaatgc tgttccgatg aaatcgcaaa catcattctg gaagccgtga aacgttacac
     9001 cagtacggag cctgctcatg caacacagca ttaaagactt gtggttgtat ccctttccag
     9061 aaattgatgt ggttcataca caggagccac tgttaccgga accggaatta acaacgcccg
     9121 gtcgatgtat ctgctgccgg caaaacgtca ggcaccgttt cagactggat gactcatggc
     9181 cactcagaca gcttaccgac acaatcagtg acacacgagt tcgtcttaat aaggccacag
     9241 aacatctgga taagttaaaa aagcgtggtg aaccagtcgc aaccggcgaa aaggaaaagt
     9301 acaacacggc agtaaaagcc gctgaacggg cgcttgagca ggcccgtctg tctgccagac
     9361 gtctttcact ccgccatgta cagaaagctg aaatcaccag tacagagtcg ttgtcagaga
     9421 aagagcagga actgtttcat gaagacggcc cgccttacag cttatgtgct ttctgccacg
     9481 cctggcattc actgaatggt tatgccgcag cacagggcgt tatggtgtgg ctacccgacc
     9541 ttcatccatc cacggtcgtg gcccttaacc gtcgttcatt acaggaagtt ttcagtaatg
     9601 ataagttccg ggtccggcgg ggacgggaag cgctctctgc actgatgcaa aacagactgg
     9661 cggttgagga taaattccgc agtttccgcc cggccgattt cgctgacgtt tttcgtcgct
     9721 atccaccttc cggtcgttcg cccctgcgtg aaaaaatgaa cgggattgcc ctgattctga
     9781 caccagattc attcatcaaa aaagaatacg ttgattgatt tttcttcatg ccactccagc
     9841 gcggggtggc attaaaaaaa tcaaactgca caaacctccc tcctgtaagt ccggagttcc
     9901 ctcacctatg aacataaacc gtttaatctt cactatcgaa gactgtctga acacgctatc
     9961 ccgtttctct gtcgcaagca gttttgttga gtattgcgat ttacgtactg taattggtct
    10021 ggacagacag gatcgtgaac gtcgcccatg gctgaattcg ccctatattg cagccacaaa
    10081 gcgtggagaa tatctctctg tctttgaagt cagcggtgct ttcagggaaa tggatgaagc
    10141 atccgaccag actggcccag gttcactgga atcgctgatc accagcatga gtgacagcct
    10201 gaatacggca tataaaaaca gcggtcataa aatcagcttc gtttttgagc gtgatcctga
    10261 catggggaaa gaagaaatcg aagacatggt cgccccacaa aagcgctcac tggctaatac
    10321 aggtatccag ttacaggatg tggtcgatga gaaagtcaca acactgtccc catggctggt
    10381 ccgggagcgt tgctggctgg cgatctggag tggacccgac cttatcagca acagcgatcg
    10441 caccgcccat gatgagcttg tcagaagact ggctgaacgg gtaccgaaag cacgctttgc
    10501 ccagtccccc tggcagtgga ccttatctgc actgaaaatc cgccacgaag cttttcttga
    10561 taatgtggaa caggcactga gacattcatc tgatggcctg attctccggc tactggatat
    10621 acacgaggtc gggcgtgaaa tacgtcgtca gacagaacgg cacagcacac cgcgtaactg
    10681 gcaaccccac ctgcctgaag atgcccagcc agcgggatat cgctggactg acgatgaatc
    10741 cgtactacat gctccttcat tgcatttgca gttattcaac actcaggtga ccacacaggg
    10801 caacctggtt caggcgggtg gactctggca cggaatggta tcgattactt tacctccgca
    10861 aaatctgcag acgtttaacg aactggtccg tgccgtcccc cgcgcagtcc cctggcgcat
    10921 cagaatggat ctgatgcccg gcgggatgaa ggctcttaat ctgaagaaaa cgcttctgac
    10981 ttacagcagc ttcatttctg ccgttcgtcc aatgtatgaa tccgttatga ccctggcggc
    11041 aacggatgaa aaagaacctg tctgcataat gaccatcatg gcctccacgt ggggaaaaac
    11101 acgtgaaata tgcacacgaa accaggccat tctgaaatcg gccattgaag gctggggcgt
    11161 atgtggcacc acgacaacat ttggcgatcc ccgacgcgcc tgggtgaata ccattctcgc
    11221 ggcatccgga ggctccggcc cggttcccct gtatcccccg ttgtcacatg ccatctcgct
    11281 tttcccgctt aacagggcag gctctgtctg gcgaggcaaa gggaatctga tgttacatac
    11341 agaagacggt tccgcatttg aggttggtct ggcgtcatcc caacaaaata aacatacaga
    11401 actggcacca ggcgatccgg gactgggtaa atcagttctg attaatacgt tgtcggaaat
    11461 acagatatca tctgctcaga aaaacctgcc cttcattgcc tatatcgata agggatacag
    11521 tgctcaggga ctggttcagc ttattcgcga cagtcttcct ccggaaagaa aagacgaagc
    11581 tgtcggtatt attctgagca atgatccgga atacacccgt aacctttttg acgtgatgta
    11641 cggcgctaaa aagcctatta cgccggaaaa gaatttcatg tcatctgtgc tgtgtgctct
    11701 ctgtgtcgat accggtaccg gtcaaccctg taaccccgga gacacccgac aaattatcaa
    11761 ccagcttatc gagctggctt ttaaagagta cggtgaaaat aatccgcgac tttatcgcgc
    11821 ctcaacggaa gaactggtgg attcagcact gcaggactcg ggactgtatg aaaaacatga
    11881 tgcaacctgg tgggccagat caacctggtt cgaagtccgg gatatgctcc acaatgccgg
    11941 gtatatcatg gcggctcagc gggcacatta tcaggcaatg ccccagcttc ccgaagtgtc
    12001 ctcgatgctg ggccatacca gtctcaggga tgttttcggt actgttcagc gtgatggttc
    12061 aaacgaactt ctgctggact acatccgccg cgctctcgag cagggacata acgactaccc
    12121 catgatatcc gggtataccc ggtttatgat caacccggaa acccgggtca tcgctgtcga
    12181 tctgaataat gttgccggtg acaaaacgcc tgcaggcagg cttaaaaccg gcatcatgta
    12241 cctgcttgca ggtcagatcg ccggtggtga cttcaccctg ccacaataca gggatgaagt
    12301 gttaaagcag cttccacggg aataccatga aatcgctctt aaacgtatta accaacttga
    12361 tcaggaggtt aagacaaaag tttatgatga actgcataac gcccggggca ttgacttcat
    12421 ctgggaaaat ctggataccc aggagcgtga gcaacgcaag tttgctatcc gcacggttct
    12481 gagtacgcag tatctgaggg actacccgga aagcgttctt aagtcagcaa atactctctg
    12541 gttactgcgc tacaaacccg aagatatccc cgtactgagg gataacttca acgttcctga
    12601 atttatgctg aaacgcttcc tgaaaatgcc ggaaggcccg gcaccagatg gtagtggtgt
    12661 tcctgttctg ggagtattca gggtaaaaag tggcacgctt gcgagaatac tcaaatttac
    12721 cgtcggtcca ctggagctat gggcgctgaa ctcatcgcca aaagacagtg ccctgcgtaa
    12781 aacactgaca aataagttag gttcagttcg ggcacgtaag attctggccg aaaacttccc
    12841 cagaggttct gcaacgtcat taattgagca ccgggcgggc caacataaca gtgacaatgt
    12901 tattgaagaa ctggcctcgg agctcatccg aaaacaggga tacaatctct gatgtgtaca
    12961 attcatatca ccccacgctg ccggagacct gccagagggc aggtatttct taaaaaactt
    13021 gaggtaaaag gtgacagttt ctcattcact ctgccgtcac gtgtcttcag gttacatccg
    13081 gcccccaatc ccagagtgat atacgcctcg gatgccagaa cacagggaaa ggtcacaggc
    13141 atgcggctgt tgttgccgtc cagcacagat gagctgataa caacggcaat atggcaggtt
    13201 tctgtcccgg atgaagcacc attcgacgtt cagcatgtca tccagtggat actcccttca
    13261 ccgctggctt cgctgatgtc cgatgatacc tggttatggc catccgttcc cggaacgccg
    13321 gcactaagtg ccggtgactg gcctgttatg gatactgacc cttccacctt acggcgcgtc
    13381 agacgtcgtt ctttcagcat catcgataac cggaacagga aaggttatat caccaggcga
    13441 tatcagtgtt tcccactggc cccactacct gaagacagaa aatacgacag ccttcctgac
    13501 cttgtgctcc atcccgagca ggagacaata aaatgcaaag aaaaacatta ctcgcagccc
    13561 ttatagccac cctgagtgga acggcatgcc aggctcatgc ttattcagtc accgtcgtcg
    13621 ccagccggcc tgttgaagag caggtcatcc ccagaatgga agccatcaaa gatgtccttg
    13681 gcaacatact gagtacccag acagccacgg gtaccgctat caatcagaac tctgaaaagc
    13741 tggcaagtgt cattgcccag aacggacagg cgacacgtca gcagatgatt ttcagtaatg
    13801 aaactcagcg cctcgaggag gcacggaaaa gcttcaccgt gccagactcc atctgcagtg
    13861 aatccgcatc agggattgca acagaaagca aaagtgcctc agcatccgca gcctcaaaac
    13921 tcagtaaagg tggcggtgtg agcaaccgta gcatccgcga ccgtctggcc agcgcagcaa
    13981 actctcccgt gcgggaagct tatgatggcg cggccataca cgccagttac tgtacagaag
    14041 cagaatatgc acgttttgga ggaaccgccg tttgtccttc tgtgggcgaa ataccaggtg
    14101 gagacagcca ggtacgctcg atttaccacg gcgcaggtac agcggacaca cctgcggcac
    14161 tgacatggga tcagaagcaa atagatgcag cgactgctta catgaaaaac acttcccgcc
    14221 cctctgccgg tcgtgctctg ggtaaaggag aagtgaatac ccagagtggg cgaacctacg
    14281 tcggactcca gaacgaatac aacggcatca ttgattctgc gtcgaatccc caactgacgc
    14341 tgattgctga cagcacgcct aatgaaagca cacgtaaagc actggctgaa acactgcagt
    14401 cagactcagc ggccgcttac ttcgaccagg tggcttcacc agaagcaaaa gcacggggat
    14461 atatgagtac ccgtgagttt gaagcttttg aggctggccg gcgctacgcc aacacagcat
    14521 acctggtgga tttacaggaa atgcaggggg ataacctgct ccgtgaactg gtaagaatca
    14581 ctgcgcaaat gaactggcag ctgaacgatc tgaaagaaca gatccgccag ggtaatgtta
    14641 tctctggcca gcaactggct ctgactgccc gccagtatta tgaaaaacaa ctcggtagcc
    14701 ttgagaaaac aatcaatcag gcaaacgcac gttgaaagct gacgtcagaa caggactgaa
    14761 aaaatgaata agttaccgga aaattcagac cagacgaaaa acgtcaaaaa atcaccatcc
    14821 ataacccgca tagccacccg ggggattatt aacatttttg ttcctctgat ggagactcgc
    14881 ctgataacga attctgtaca gcatatgctg gaacaacaaa aacgcaggct ggacagattt
    14941 cgtcaggtaa ataataaaaa ggcgcaactc cacctcacgt gggaagaagc tctgcaggcc
    15001 agccatatgt cagtagacga tctggatcgt cgtttccgcc ggcgcagaac agtatggcga
    15061 ttctgttgct ggagtctgct ggccatcgct ttgtttctca gtggtatgct ctttgccgcc
    15121 tcttcattac ctcttacgac tctggtacgc gccatcagca ctcttgtgtt gatactgtca
    15181 ggcgtcgctc tttgtgcttc cagagcactg attgttacat accgcctctg gcagttgcat
    15241 gaacgaaaag tcagcgaacc cgagcagggg actttccgtg attttctgaa cgaccgcaat
    15301 ggctggcgca atgccacatt aattgccgta accagtaaac aatactgatc acgtccagat
    15361 gccagtacca ctggcatgtc gccatccccc ttaattaaat cctgtgtggc ctttgcccat
    15421 acgcaattct attccggagt tactgtgaaa attttacttc gcgcactctg tgcaggcctt
    15481 gctatatcct cactgccggc aatggcttca gtgacctatc aggatattgt gtctgccgca
    15541 accaatcctg acgatctgtc ccgtcaggcg ctggtcacga tattcggtga tgtagtcacc
    15601 aatccactgt caaccagcgc ccccactctg atcggaagca tgttcggggc gttcaacagt
    15661 atcatcgccg tacttgccgt tgtctggttt atgttcattg gtatccgtca tgttgtgcgt
    15721 tcggggcatc agggacaggt attcagcacc ggacgcgata tcgtcggcac actgagtgtc
    15781 gttgcaggtt ttctgatgat cgttccaacc ggaaatggct ggagtatcgc tcagctcatt
    15841 atgttatggg gcgcatcgat aatgggggtt gggtccgcca atgtgatggt gcaacttgcc
    15901 gctgataata tcgcaaatgg ttattcaatg actgtacagc cagttcaggc atccacacga
    15961 acagcagcgc gtggcatttt tgaaatggaa ctgtgtaagt atgcggttaa cgcaggacta
    16021 aatgatttta atcagacggt caaatcctcg acttcactga tgacagaatc agctaaaacc
    16081 gccagtggta attacactgt cacggtatcc aatggcagcg gtatctgtgg ttcagccagc
    16141 ctgtcagtag aaggaaacgg aactacagat caatcgacga ttggaaaatt ctttaatccc
    16201 ttcagtaaaa acgaatacag cggtgttatc tcagcacaac gtgcagccat ggataatatg
    16261 attagcgaca tggacaacgc tgccagtgag tttgtgacaa catttctgga aaaaagaaat
    16321 tctggcaacg gaacattgcc ggatatcgag acacggattc agagagcagc cgacgagtat
    16381 gaacgcgctg tacaaaaatc gctacctatt gataacggag aacaatccag gaaagaagct
    16441 ctcaagtctt atctcacaac ctatggatgg gtgacgcttg gcgcgtggta tcagaccttt
    16501 gccactgcta accagcgtct ggcagagctg gctgacagag ctccagctgt aacttcaatg
    16561 tcatcacttg gggaagttgg cgatactgat ttatttagtg ctgttatggg ggcatacaag
    16621 actcaattac agaactccac atatactcca acgataggaa cgataacaac tcaagatgaa
    16681 agtaatgcag ctaatagcac agacccacaa agtgtggtca tgaaaagtat agcgccaacc
    16741 atattaaaat ggacaaacca agctgctact gaatggtcag gaacaggaac aaccagcaac
    16801 caagttaacc cattaattaa gatgaaaaac atcggcgatt atacattggg tacaacagaa
    16861 attctctgga caggatatac aactgtcaga gttctcgcga caatgggtga taactctatt
    16921 ttcggaaagg ctgttaattt gttctctgga ttacctaaag gatttgttgc tctgctggat
    16981 gctgcagctc ccccaatata tttcttgttg ttcctgttgt tttgtgcggg tttttcctta
    17041 tcaatttacc taccatttat accattcatt ttctggatga caggcattgg gaactggata
    17101 gtaagcgtat taataggatg caccgccggg cctctgtggg cagcaacaca tctaggaaca
    17161 tcagaggaca gaggaagtcg agcagcttac ggatatatat acttaattga cagcatgata
    17221 agaccacctc tcatggtatt cggtttcttt tttgcctctg tcgccatcat tgccgtcggc
    17281 acaatattaa actcactgtt tggcgcagca ctagttaacg tacaggtaaa atcattaaca
    17341 gggatattca gccttgccgg gttcctgcta atttacgccc gtatgtgtac aacgtctgta
    17401 gctgcaatat ttgctttaca agcatatctt cctgaccatg ttatcaattt cttgggtggt
    17461 agagatggtg taaatactct tggcaacctc acctcatcag taaaagatat ttttgcaggt
    17521 tctaatagaa atattagaca cagccctgga gttagagagg a
//
LOCUS       DAPGRN010000100        17493 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPGRN010000100 DAPGRN010000000
VERSION     DAPGRN010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.101"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(438..1634)
                     /gene="hemY"
                     /locus_tag="RHW33_003532"
     CDS             complement(438..1634)
                     /gene="hemY"
                     /locus_tag="RHW33_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV1647001.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1637..2818)
                     /gene="hemX"
                     /locus_tag="RHW33_003533"
     CDS             complement(1637..2818)
                     /gene="hemX"
                     /locus_tag="RHW33_003533"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV1647002.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAETTEAKPAPAPQADTPAAAPQG
                     E"
     gene            complement(2840..3580)
                     /gene="hemD"
                     /locus_tag="RHW33_003534"
     CDS             complement(2840..3580)
                     /gene="hemD"
                     /locus_tag="RHW33_003534"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV1647003.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3577..4518)
                     /gene="hemC"
                     /locus_tag="RHW33_003535"
     CDS             complement(3577..4518)
                     /gene="hemC"
                     /locus_tag="RHW33_003535"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV1647004.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4905..7451
                     /gene="cyaA"
                     /locus_tag="RHW33_003536"
     CDS             4905..7451
                     /gene="cyaA"
                     /locus_tag="RHW33_003536"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV1647005.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLMPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7491..7811)
                     /gene="cyaY"
                     /locus_tag="RHW33_003537"
     CDS             complement(7491..7811)
                     /gene="cyaY"
                     /locus_tag="RHW33_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV1647006.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEKAATQQAGET
                     VSFR"
     gene            complement(7937..8116)
                     /locus_tag="RHW33_003538"
                     /pseudo
     CDS             complement(7937..8116)
                     /locus_tag="RHW33_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            8275..8478
                     /locus_tag="RHW33_003539"
     CDS             8275..8478
                     /locus_tag="RHW33_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1647007.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8515..9339
                     /gene="dapF"
                     /locus_tag="RHW33_003540"
     CDS             8515..9339
                     /gene="dapF"
                     /locus_tag="RHW33_003540"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV1647008.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9336..10043
                     /locus_tag="RHW33_003541"
     CDS             9336..10043
                     /locus_tag="RHW33_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV1647009.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLIEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10040..10936
                     /gene="xerC"
                     /locus_tag="RHW33_003542"
     CDS             10040..10936
                     /gene="xerC"
                     /locus_tag="RHW33_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV1647010.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10936..11652
                     /gene="yigB"
                     /locus_tag="RHW33_003543"
     CDS             10936..11652
                     /gene="yigB"
                     /locus_tag="RHW33_003543"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV1647011.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11736..13898
                     /gene="uvrD"
                     /locus_tag="RHW33_003544"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11736..13898
                     /gene="uvrD"
                     /locus_tag="RHW33_003544"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV1647012.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(13943..14845)
                     /locus_tag="RHW33_003545"
     CDS             complement(13943..14845)
                     /locus_tag="RHW33_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HDV1647013.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVGHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELPYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSEKKYEGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCDK"
     gene            complement(14928..15692)
                     /locus_tag="RHW33_003546"
     CDS             complement(14928..15692)
                     /locus_tag="RHW33_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV1647014.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            16062..17012
                     /gene="corA"
                     /locus_tag="RHW33_003547"
     CDS             16062..17012
                     /gene="corA"
                     /locus_tag="RHW33_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV1647015.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(17054..17476)
                     /locus_tag="RHW33_003548"
     CDS             complement(17054..17476)
                     /locus_tag="RHW33_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV1647016.1"
                     /translation="MRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINWYLKHQNEIKN
                     QHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINE
                     YPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKKDNF"
BASE COUNT         4016 a         4544 c         4626 g         4307 t
ORIGIN      
        1 aattaatata tgtgttatta attgaatgaa ttttatcatt cataataagt atgtgtagga
       61 tcaagctcag gttaaatatt cactcaggaa gttattactc aggaagccaa gaggattaca
      121 gaattatctc ataacaagtg ttaagggatg ttatttcccg attctctgtg gcataataaa
      181 cgagtagatg ctcattccat ctcttatgtt cgccttagtg cctcataaac tccggaatga
      241 cgcagagccg tttacggtgc ttatcgtcca ctgacagatg tcgcttatgc ctcatcagac
      301 accatggaca caacgttgag tgaagcaccc acttgttgtc atacagacct gttttaacgc
      361 ctgctccgta ataagagcag gcgttttttt atgtgtcagg aaggccccgg aggtgcttgc
      421 ctccgggtga gaaggaacta ctgtggtggg ttgttctgca acgttaacat caaaccgtcg
      481 cgacgcatcg ctgcagcttc ttctggtttg tgcagtctgt ccagcgcgtc ggcaagccat
      541 gcgtaatcgt aggcgtccgg acgttgtttc agcgctgcgc ggaaggcgag cgatgcttcc
      601 tgccattcgc cgtgcttcat cagtgactga cccagtgtgc tccacaacag cgggcgatcg
      661 ccgacgtttt tgatttgctg gcgcagcact ttttcaagct gttcagggtt attggttttc
      721 agacgcggga tcggcagcag taggcgatcg tcatactggc gtttcaggcc gtcgatgata
      781 atttgctggg cagtatcatg atcgtcacat tcaataagat gttccgccat tgccacctgc
      841 aacgctacct gatgacgcgt tttccggctt tggtttttcc accagttacg caaaccttcg
      901 ctaccgttat cggcacgcgc ctgatccatc aggccaatcc atgcctgttg ttccagcatt
      961 gcacgatgtt cttcatcacc aacatgggct ttcgccattg atgggataat atccagcagc
     1021 gaactccatg cacctgtgcg gatatacgcc tgttccgcca gacgtaatac ttccggatgg
     1081 cgtggcgtaa cttccagcag cttatccacg ccgtggcgtg cagcatggtt ttcattacgg
     1141 gccagttgca gacgtacacg ggtgatttct accggaatgg tgtcattgcc ggccagctcc
     1201 gctgcgcgtt ccagatgttg gttggcgcgc gcttcatcac cacgttgttg cgcggcttcg
     1261 gcagccagta gatagttcac caccggttgt tcagcgtgat cggcattttt cgccatcagc
     1321 ttttcaactt gctgataatc gccttccgcc agtttcagca gcgcctgttc ggtctgctta
     1381 cgtgcacggc gacgcttacg cccgacaaac cacccacggg tgtgcgcgcc agtgcggaag
     1441 atccgccgca gtagccactc aatggcaaac agcactacca tcgccagaat caatatgatc
     1501 gccaggcccg tgacgctggt ttcgatattg tagttgtcgg tctggatcag cacataaccc
     1561 tgatgaccgg caatcatcgg gccaaccacg atccccgcaa tcagcaacac aaagagcaat
     1621 aacactttta gcatggttat tctccttgcg gcgcggctgc cggagtatca gcttgcggtg
     1681 caggtgcagg tttagcttcc gttgtctccg ctgccggttg tgccagcagg ttacgcacgc
     1741 gagtctgcat cagtttttcc agcatcgcct ggctttgcag ggtttccgga agatccatcg
     1801 agatattttg ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat
     1861 cagtatcgta gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct
     1921 cttcctgatg acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc
     1981 gcagatagat atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa
     2041 tcgtaatgaa gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc
     2101 tgatggaact ggaaagctct tcaccgtcgg aatccatcgg cgaaccatcg ctgtcattat
     2161 cggccagacg caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt
     2221 cataatccac ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa
     2281 cggtaatcaa actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg
     2341 ctgcagcggt cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa
     2401 aatcggcctg agccagcagc caggttttag catcgctgcc ggaaatggtg gcgacctttt
     2461 gttggacttc atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct
     2521 gtgcagcttg ttgcttaata ataccttcca gctcggcttt ttggctctcc tgggcttttt
     2581 gcaatgccgt cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt
     2641 gtttacccca gccatacaaa ccgacgcccg ccgccagagc aatagcgata gccaccgcgc
     2701 tgagaatcaa tgcggtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg
     2761 tgtccacggc ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta
     2821 tggcttcctg ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat
     2881 cggcgacctt aatgtcttgc cagcccagtt cccgggcgag tttcgccaaa cgctcactga
     2941 cgaccaatag tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc
     3001 agagttgctg caacatttca ccgctggtaa caacgaccgt cgtcacctcg cgggattgcc
     3061 agcgcatcgc ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa
     3121 aagtgacctc agcaccgcgc gccgtcaggg tatccccaat tagctcacga ccgccattgc
     3181 cacgtaatat cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga
     3241 cttcgctgat ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg
     3301 ccagtgcggt ggtgcgtcca atggcgaaat aagtaggtag tcggggccat ttacgatctt
     3361 gctgatgcag ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat
     3421 cgctctcccc cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa
     3481 actcaatcag cggaaaatgc caggccacct gccccagtgt gcgcagacgg ctcactaact
     3541 cttctccagc gggagacggg cgggtgacaa ggatactcat gccggggcgt ctccgttata
     3601 gacttcagcg aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc
     3661 catttgttcg gcattttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc
     3721 cggcgcgccg accagcgcac gcagccagat ttcgccatca ataagctcgg cgtagctacc
     3781 aattggcacc tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg
     3841 cagtgcagtt tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag
     3901 gcggcattca ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggcaa
     3961 cgcggcgcga atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat
     4021 ggcatcgtat tcgccgttat ccagtttgct caggcgagtg ccgacgttgc cgcgcaggga
     4081 gcggataatc agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt
     4141 cccgacgata ctgcctgccg gtaacgcatc cagactgtca tagttattgg acacaaaggc
     4201 atcgcgagga tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac
     4261 cggcacatct ttcattgaat gtacggcgat atcggcgcga ttttcgagga gcgcgacttc
     4321 cagctctttt acaaataagc cttttccgcc gactttcgcc agcggcgtat caagaatcac
     4381 atcgccgcgc gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa
     4441 cttgtctttg acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct
     4501 taaaacattg tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc
     4561 ttgccagagt gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga
     4621 ggtctggcag tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa
     4681 taaacggtgc tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta
     4741 aattgatcac gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag
     4801 tggtaacggt tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt
     4861 ggcggaatca cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg
     4921 agactctgaa acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg
     4981 ctatggggcc tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc
     5041 atccgctaat gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc
     5101 ctgatgaaac tcaacgccat tacctgaacg agcttgaact gtatcgtgga atgtcagtac
     5161 aggacccacc gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt
     5221 ccgtagggca aagttgttcc tctgaccttg atatctgggt ctgtcatcaa tcctggctcg
     5281 atagcgaaga gcgccagtta ctacaacgta aatgcagcct actggaaagc tgggccgcct
     5341 cgctgggcgt ggaagttagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa
     5401 gcggcagcct ggggggcgaa gattgtggct ccacccagca tatactgctg cttgatgaat
     5461 tttatcgtac cgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg
     5521 acgaagaaga gcattacgat gactacgtaa tgacgctcta cgcacaaggc gtgctgatgc
     5581 caaatgaatg gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg
     5641 ccagcctttg gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac
     5701 tgctgctgga agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca
     5761 aacagcgttt gcacgacggc gagattgtat cgtttggcct cgatccgtac tgcatgatgc
     5821 tggagcgtgt tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac
     5881 gccgctgctt ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgcgttggct
     5941 ggcgtcgcgc agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg
     6001 caatgctcga taaccgcgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc
     6061 tgctcgacgc gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc
     6121 ttagcgtctc cgccagtccg caggatatcg gcgtgctgac gcgtaagctg tatgccgcgt
     6181 ttgaagcatt accaggtaaa gtgacgctgg taaacccgca gatttcgccc gatctctcgg
     6241 aaccgaatct gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc
     6301 tgtataaccg cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc
     6361 gttacctgaa taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc
     6421 gcttgtatat taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg
     6481 acgtgtcgca ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc
     6541 cgtgtgagat ccgccatctg gcgattatcg ttaacctgga atatgacccg acagctgcgt
     6601 tccgcaatca ggtggtgcat ttcgatttcc gtaagctgga tgtcttcagc tttggcgaga
     6661 atcaaaattg cctggtaggt agcgttgacc tgctgtatcg caactcgtgg aatgaagtgc
     6721 gtacgctgca cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca
     6781 aaatgcatca ggacgccgca ccgccagata gcgtggaagt cttctgttat agtcagcatc
     6841 tgcgcgggtt aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgtc
     6901 tttccagcac ccgccaggaa accggacgct tcaaggcgct gcgcgtttct ggtcaaacct
     6961 gggggttgtt cttcgaacgc ctgaatgtat cggtacagaa actggaaaac gccatcgagt
     7021 tttatggcgc tatttcgcat aacaaactgc acggcctgtc agtgcaggtt gaaaccaatc
     7081 acgtcaaatt accggcggtg gtggacggct ttgccagcga agggatcatc cagttcttct
     7141 tcgaagaaac gcaagacgag aatggcttta atatctacat tcttgacgaa agcaaccggg
     7201 ttgaggtgta tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct
     7261 tctactcgtc atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc
     7321 cgcagttcta tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgcacca
     7381 aatctatcgg taacatgccg cctgccaatc aggatcacga tacgccgcta ttacagcagt
     7441 atttttcgtg atgaacgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa
     7501 ctgactgttt cacccgcctg ctgcgtcgcc gccttttcca gcaaatccca gaaggtttcg
     7561 ccgctgcgat cgcaaatcca ctcatcgcct ttcagatcaa aatggtagcc gccttgtttg
     7621 gttgccagcc atacctggtg cagcggttcc tggcggttga taatgatttt gctgccgttt
     7681 tcaaaggtaa tggtcagtac accgccgttg atttcgcagt cgatatcgct gtcgccatcc
     7741 cagtcgtcca ggcgttcttc aatggtcagc cagagttgat cagccaggcg atgaaattca
     7801 ctgtcgttca ttgttgtatc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc
     7861 agggcttcaa agtttgcacc tctgcggctg cgttccggca tgattcagcc gtcaccggaa
     7921 taataatgtc tctgtgtagc gaaagatttg tctcttcatt tgggcgcagg tacaccacgc
     7981 ctttcccctg tttctggtaa acattatgat caggttcacc gagcgagcat ccccacgctg
     8041 acggaactaa aaaagacaac aaacaaacca cattgcgata gtacataaag ccatcctggc
     8101 gcgaggtgtt gatcacgaaa ctgccagcag aacataaatc ctcacgagta agcgttatac
     8161 tcgcagcatt tcctcacttt tcagacttca taaagagtcg ctaaacgctt gcttttacgc
     8221 cttctcctgc gatgatagaa agcagaaagc gatgaacttt acaggcaatc cataatgaaa
     8281 aacgtgttta aggcactcac tgtattactt actctcttca gcctgacggg ctgcggtctg
     8341 aaaggtccgc tctatttccc gcctgcagat aaaaatgcac cgccgccgac caaaccggta
     8401 gagacgcaaa cgcaatccac ggtgccggat aaaaacgatc gcgccactgg cgatggtcca
     8461 tcccaggtga attactaaaa gtcagtttct gtacccgcgt gattggagta aatgatgcag
     8521 ttctcgaaaa tgcatggcct tggcaacgat tttatggtcg tcgacgcggt aacgcagaat
     8581 gtcttttttt caccggagct gattcgtcgc ctggcggatc ggcacctggg ggtagggttt
     8641 gaccagctgc tggtggttga gccgccgtat gatcctgaac tggattttca ctatcgcatt
     8701 ttcaatgctg atggcagtga agtggcgcag tgcggcaacg gtgcgcgctg ctttgcccgt
     8761 tttgtgcgcc tgaaagggct gaccaataag cgcgatatcc gcgtcagcac cgccaacggg
     8821 cggatggttc tgaccgtcac cgatgatgat ctggtccgcg taaatatggg cgaacccaac
     8881 ttcgaacctt ccgctgtgcc gtttcgcgct aacaaagcgg aaaagaccta tattatgcgc
     8941 gccgccgagc agacaatctt atgcggcgtg gtgtcgatgg gaaatccgca ttgcgtgatt
     9001 caggtcgatg atgtcgatac cgcggcggta gaaacgcttg gtcctgttct ggaaagccac
     9061 gagcgttttc cggagcgagc caatatcggt tttatgcaag tggttaagcg cgagcatatt
     9121 cgtttacgcg tttatgagcg tggggcagga gaaacccagg cctgcggcag cggcgcgtgt
     9181 gcggcggttg cagtagggat tcagcaaggt ttgctggccg aagaagtacg cgtggaactc
     9241 cccggcggtc gtcttgatat cgcctggaaa ggtccgggtc acccgttata tatgactggc
     9301 ccggcggtac atgtctacga cggatttatt catctatgaa gcaaccaggg gaagaactgc
     9361 aggaaacact cacggagctt gatgaccggg cggttgtcga ttatctgatt aaaaatcctg
     9421 agttttttat ccgcaatgcg cgcgcagtag aagcgatacg tgtgccgcat ccggtacgcg
     9481 gcaccgtttc gttggtcgag tggcacatgg cccgcgcacg taatcatatt catgttctgg
     9541 aagagaacat ggcgctgttg atagaacagg ctatcgccaa cgaaggcctg ttttatcgcc
     9601 tactctacct gcagcgcagt ctcaccgccg ccagcagtct cgacgatatg ctgatgcgct
     9661 ttcaccgctg ggcgcgcgat ctcggcctgg caggtgcgag tctgcgcctg tttccggatc
     9721 gctggcgctt aggtgcgccg tcgaaccaca ctcatctggc attaagccgt cagtctttcg
     9781 aaccgctgcg tattcagcgt ttggggcagg aacagcacta tcttgggccg cttaacggac
     9841 cagagctgct ggtggtgcta ccggaagcga aagcggtggg atcggtggcg atgtcgatgc
     9901 tgggaagcga tgctgatttg ggtgtcgtgc tgtttaccag tcgcgatgcc agtcactatc
     9961 aacaagggca gggaacgcag ttacttcatg aaattgcgct gatgttgccg gagcttctgg
    10021 agcgttggat tgaacgcgta tgaccgattt acacaccgat gtagaacgct acctacgtta
    10081 tctgagcgtg gagcgtcagc ttagcccgat aaccctactt aactaccagc gtcagcttga
    10141 ggcgatcatc aattttgcca gcgaaaacgg cctgcaaagc tggcaacaat gcgatgcagc
    10201 gatggtacgc aattttgctg tacgcagtcg ccgtaaaggg ctgggagcag caagcctcgc
    10261 gttacggctt tctgcgctac gtagcttttt tgactggctg gtcagccaga acgaactcaa
    10321 agctaacccg gcgaaaggtg tttcggcacc gaaagcgccg cgtcatctgc cgaaaaacat
    10381 cgacgtcgac gatatgaatc ggctgctgga tattgatatc aacgatcccc tcgctgtacg
    10441 cgaccgtgca atgctggaag tgatgtacgg cgcgggtctg cgtctttccg agctggtggg
    10501 gctggatatc aaacacctcg acctggagtc tggtgaagtg tgggtaatgg ggaaaggcag
    10561 caaagagcgc cgcctgccga ttggtcgcaa cgctgtggcg tggattgagc actggcttga
    10621 tttgcgcgac ctgtttggta gcgaagacga cgcgcttttt ctgtcgaaac tgggcaagcg
    10681 tatctccgcg cgtaatgtgc agaaacgctt tgccgaatgg ggcataaaac aagggctgaa
    10741 taatcacgtt cacccgcata aattacgtca ctcgttcgct acgcatatgc tggagtcgag
    10801 cggcgatctt cgtggtgtgc aggagctgct gggtcatgcc aacctctcca ccacgcaaat
    10861 ctatactcat cttgattttc aacaccttgc ctcggtgtac gatgcggcgc atccacgcgc
    10921 caaacggggg aaataatgcg tttttaccgg cctttggggc gcatctcggc gctcaccttt
    10981 gacctggatg atacccttta cgataaccgt ccggtgattt tgcgcaccga gcgagaggcg
    11041 cttacctttg tgcaaaatta tcatccggcg ctgcgcagct tccagaatga agatctgcaa
    11101 cgcctgcgcc aggcggtacg ggaagcggaa cccgagattt atcacgacgt gacgcgttgg
    11161 cgttttcgtt cgattgaaca agcgatgctc gacgccgggc tgagtgccga agaagccagt
    11221 gcaggcgcac acgcagcaat gattaacttt gccaaatggc gcagccgaat cgacgtcccg
    11281 cagcaaactc acgacacctt aaaacagctg gcgaagaaat ggccgctggt ggcgatcacc
    11341 aacggtaacg cccagccgga actgtttggt ttgggggatt attttgagtt tgtgctgcgc
    11401 gctggcccgc acgggcgctc aaaaccgttc agcgatatgt actttctggc tgcggaaaaa
    11461 ctcaacgtgc cgatcggcga gatcttacat gttggggacg atctcaccac tgacgtgggt
    11521 ggggcaattc gcagcggaat gcaggcttgt tggatcagac cggaaaatgg cgatctgatg
    11581 caaacctggg acagccgttt actgccgcat ctggaaattt cccggttggc atctctgacc
    11641 tcgctgatat aatcagcaaa tctgtatata tacccagctt tttggcggag ggcgttgcgc
    11701 ttctccgccc aacctatttt tacgcggcgg tgccaatgga cgtttcttac ctgctcgaca
    11761 gccttaatga caaacagcgc gaagcggtgg ccgcgccacg cagcaacctt ctggtgctgg
    11821 cgggcgcggg cagtggtaag acgcgcgtac tggtgcatcg tatcgcctgg ctgatgagcg
    11881 tggaaaactg ctcgccatac tcgattatgg cggtgacgtt taccaacaaa gcggcggcgg
    11941 agatgcgtca tcgtatcggg caactgatgg gcaccagcca gggcggcatg tgggtcggca
    12001 ctttccacgg gctggcacac cgtttgctgc gtgcgcacca tatggacgcc aatctgccgc
    12061 aggatttcca gatcctcgac agtgaagacc agctgcgcct gcttaagcgt ttgatcaagg
    12121 cgatgaacct cgacgagaag cagtggccgc cgcgccaggc aatgtggtac atcaacagcc
    12181 agaaagatga aggcctgcgt ccgcatcata ttcaaagcta cggtaacccg gtggagcaga
    12241 cctggcagaa ggtgtatcag gcgtatcagg aagcgtgcga tcgtgcgggg ctggtggact
    12301 tcgccgagct gctgctgcgc gctcacgagt tgtggcttaa caagccgcat atcctgcaac
    12361 actaccgcga acgttttacc aacatcctgg tggacgaatt ccaggatacc aacaacattc
    12421 agtacgcgtg gatccgcctg ctggctggcg acaccggcaa agtgatgatc gtcggtgatg
    12481 acgaccagtc aatctacggc tggcgcgggg cgcaggtgga gaatattcag cgtttcctca
    12541 atgatttccc cggtgctgaa actattcgtc tggagcaaaa ctaccgctct accagcaata
    12601 ttctgagcgc cgctaacgcc ctgattgaaa acaataacgg gcgtctgggt aaaaaactgt
    12661 ggaccgatgg cgcggacggt gagcctattt ccctctattg cgcttttaac gaactcgatg
    12721 aagcgcgttt tgtggttaac cgcatcaaaa cctggcagga caacggcgga gcgcttgccg
    12781 agtgcgccat tctctaccgc agcaacgccc agtcgcgggt gctcgaagag gcgttattgc
    12841 aggccagtat gccgtaccgt atttacggcg ggatgcgatt cttcgaacgc caggaaatca
    12901 aagatgcgct ctcgtatctg cgcctgattg ccaaccgcaa cgacgacgcg gcctttgagc
    12961 gtgtggtgaa tacgccaacg cggggtattg gtgaccggac gctggacgtg gtacgtcaga
    13021 catcgcgcga tcgccagtta acactctggc aggcatgtcg tgaactgttg caggaaaaag
    13081 ccctcgccgg acgtgctgcc agcgccttac agcggtttat ggaactgatc gacgccttag
    13141 cgcaggaaac tgccgacatg ccgctgcatg tacagactga ccgggtaatt aaagactccg
    13201 gcctgcgcac catgtacgag caggagaagg gcgaaaaagg tcagacgcgt atcgaaaact
    13261 tagaggaact ggtgacggca acgcgccagt tcagctacaa cgaagaagat gaagatttaa
    13321 tgccgctgca ggcgttcctc tcccatgcgg cactggaagc gggcgagggg caggcggata
    13381 cctggcagga cgcggtgcag ttgatgacgc tacactcggc gaaagggctg gagttcccgc
    13441 aggtgtttat cgtcggtatg gaagagggca tgttcccaag ccagatgtcg ctggatgaag
    13501 gcggtcgtct ggaagaagaa cgccgtctgg cctacgttgg cgtaacccgt gcgatgcaga
    13561 aactgacgct gacctacgcg gaaactcgcc gtctgtatgg caaagaggtt taccatcgcc
    13621 cgtcgcgctt tatcggtgag ttgccggaag agtgtgtgga agaggtgcgc ctacgcgcca
    13681 cggtaagccg gccggtcagc catcagcgta tgggtacgcc gatggtcgag aacgacagcg
    13741 gctacaagct cggccagcgt gtacgccacg ctaagtttgg tgaaggcact atcgtcaata
    13801 tggaaggcag cggcgagcat agccgtttgc aggtggcatt ccagggccag ggtattaaat
    13861 ggctggtggc ggcatacgcc cggctggaga cggtataacg ttgcgggatg cggtgcgcca
    13921 caccgcatcc ggcatcagca ccttacttat cacatttctt cacatcaata ttccagtcag
    13981 caccagacgc aacctgatgt ttctgcgcga aattatccat attcagcgcc acggaaacat
    14041 tcaataagct gttcagatac accatcggct gtccttccgg cacatcgccg aagcttgcga
    14101 catacggcat tttcccttcg tattttttct cggaaccttc ggaaatggtc acacataacg
    14161 tatcgttgag tttgattcct gcctgattca gcaactcgtc gctgatattg ctccagacgt
    14221 taccgtactg aatatcgaga atcgggatat tgcccttcac ttcacctttt gtcgctttcg
    14281 ctttctgata aggcagttcg acaacttttg ccggaagctc tggccccacc tgttcgaacg
    14341 tgatcgctcc ggaagccagg cgtgcaccgg tgtaggcata cacgtcacga ccgtggaagg
    14401 tatatgactt ttcggaacct ttcaggcggt tggttttttc atcgatttgc cggacgctct
    14461 caatacccag cgattccgcc accaaagtga gcgtaccgtt atctggtgaa acgaagtatt
    14521 gaccgttttt ggttttcagc actaccgatt tacggtctgt acctacacct ggatcgacca
    14581 cgctaacaaa caccgaacct tgtggccaat aactggcggt ctgatacagg cggtaagcgc
    14641 cttcccagat gttatacggt ggaatttcgt gtgttaaatc aaagatttta agattatggc
    14701 caacaccgaa tgcgacccct ttcatcgccg agacggcacc atctttcagg ctaaaatcag
    14761 tttgtaaaat caaagcgtta tccgcataag cggatgatga tcccagacac gttaataaaa
    14821 ataacgttgc gagcgttttt ttcatttttg tttcccttca tttatctgat gattttccag
    14881 ctgtgtagcc agccccagtt tgtctggcct cctcgagtgt acaatgctta acctttccgc
    14941 tcgacggaaa tcatggtgac aaaaggatag cgttgccacg ggattgcccc gcctttcata
    15001 tacatatgtg aaatcgtgcc atcaagataa agcagctgct caacgttcag tttcgctttg
    15061 gcataacagg caaaatcata aaaatttgtc gcctgctggc tcaacaaaaa cacggcattc
    15121 ccatgtttat taatcccaac accgttacga attttacgtg aggcgacgtt gggatgaata
    15181 cgcggattaa ttacactgtt ttccatcaac attggccctg actgcaccgc aaactgaatc
    15241 tctttactgg ttttgaaggc atccagacga acgatgccga ctttatctcc cgcgacataa
    15301 aacacgccgc caggacggat aaagaaattc ccttcacctg aagcgagatt taacgccacc
    15361 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
    15421 ccgttcatcg ccatctgcac ctgaccctga ctattcatat ccgccagcag agcatgtaaa
    15481 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
    15541 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
    15601 acagcaaaca agggaagtaa agtgagggcg agaaaaatcc gtttgagatt caaggtgatc
    15661 attcctttac caatgagtag ccgatgcgcc attataggtc ctggatgtgg gattttttta
    15721 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
    15781 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
    15841 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
    15901 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
    15961 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
    16021 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
    16081 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
    16141 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
    16201 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
    16261 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
    16321 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
    16381 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgctcg tagccagtcg atggtagacg
    16441 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
    16501 aaattgaaaa tatctatagc gacctggagc agttgagtcg ggtgattatg gaagggcatc
    16561 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
    16621 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggta cgtaaagcac
    16681 gcttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgagatatc gaatccctgc
    16741 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
    16801 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
    16861 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactaa
    16921 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
    16981 atctgtactt taagcggaag aactggttgt aatatgagtg ccggataaag ctattttttt
    17041 atccggctta tttctaaaaa ttgtcttttt taaagtcatg cggattgaaa actcgctcta
    17101 ttttattttc ttgtgttaat tgatactcag taaaatcgtt aataccaata tgagtgttac
    17161 gataagggta ttcattaata accattaagt aatttttcac gatgcaatta tcattgctct
    17221 taattgtcgg gaaacagtag agatcttcat acggcccttc gatatatttt ttatagtcag
    17281 tgaagccatt accgatatca atgacatacc aggtatgcca actaccttct ggtaggggat
    17341 gttgattttt aatttcattc tgatgcttca gataccaatt tattttatcg ctgtcagtcc
    17401 atggcagatg atcaacgacc agaaagactg aattgccagc atcagagtat gtatacaaaa
    17461 tctgtacagg acgcaatgtc cataaattat aga
//
LOCUS       DAPGRN010000101        17486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPGRN010000101 DAPGRN010000000
VERSION     DAPGRN010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.102"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..529
                     /locus_tag="RHW33_003549"
     CDS             152..529
                     /locus_tag="RHW33_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647017.1"
                     /translation="MDERFINITPTSLSFKHADLSGLSYKSARLELTLICPAIKSEVK
                     ISFDWIHSFRVTDEGDLLGMSGKQGINQTGIYRVANSTYLTWFTMQSCEIHKNKMIEH
                     YVIATSNDVIDVLSTVSPQIVYD"
     gene            645..1445
                     /locus_tag="RHW33_003550"
     CDS             645..1445
                     /locus_tag="RHW33_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV1647018.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1642..3081
                     /gene="aldA"
                     /locus_tag="RHW33_003551"
     CDS             1642..3081
                     /gene="aldA"
                     /locus_tag="RHW33_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV1647019.1"
                     /translation="MSVPVQHPMYFDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3123..4124)
                     /gene="gap"
                     /locus_tag="RHW33_003552"
     CDS             complement(3123..4124)
                     /gene="gap"
                     /locus_tag="RHW33_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV1647020.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVNGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            4313..4843
                     /gene="cybB"
                     /locus_tag="RHW33_003553"
     CDS             4313..4843
                     /gene="cybB"
                     /locus_tag="RHW33_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV1647021.1"
                     /translation="MKNKYSRLQISIHWLVFLLVIAAYCAMEFRGFLPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFELVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            5088..5261
                     /locus_tag="RHW33_003554"
     CDS             5088..5261
                     /locus_tag="RHW33_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647022.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(5333..5482)
                     /gene="hokB"
                     /locus_tag="RHW33_003555"
     CDS             complement(5333..5482)
                     /gene="hokB"
                     /locus_tag="RHW33_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV1647023.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5881..7521
                     /gene="trg"
                     /locus_tag="RHW33_003556"
     CDS             5881..7521
                     /gene="trg"
                     /locus_tag="RHW33_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV1647024.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRTLDNAYTDILNKAVKIRSTRANQLAELAHQRTRVGGMFMIGAFVLALVMTLIT
                     FMVLRQIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISMSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(7560..8483)
                     /locus_tag="RHW33_003557"
     CDS             complement(7560..8483)
                     /locus_tag="RHW33_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1647025.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLNRKEGPNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            8700..10043
                     /locus_tag="RHW33_003558"
     CDS             8700..10043
                     /locus_tag="RHW33_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV1647026.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYDQRGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            10268..11923
                     /gene="opgD"
                     /locus_tag="RHW33_003559"
                     /gene_synonym="mdoD"
     CDS             10268..11923
                     /gene="opgD"
                     /locus_tag="RHW33_003559"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV1647027.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            12063..12287
                     /locus_tag="RHW33_003560"
     CDS             12063..12287
                     /locus_tag="RHW33_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV1647028.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            12350..12889
                     /gene="rimL"
                     /locus_tag="RHW33_003561"
     CDS             12350..12889
                     /gene="rimL"
                     /locus_tag="RHW33_003561"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV1647029.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(12881..13861)
                     /locus_tag="RHW33_003562"
     CDS             complement(12881..13861)
                     /locus_tag="RHW33_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV1647030.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            13985..14977
                     /gene="tehA"
                     /locus_tag="RHW33_003563"
     CDS             13985..14977
                     /gene="tehA"
                     /locus_tag="RHW33_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV1647031.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            14974..15567
                     /gene="tehB"
                     /locus_tag="RHW33_003564"
     CDS             14974..15567
                     /gene="tehB"
                     /locus_tag="RHW33_003564"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV1647032.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFAGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            15871..16539
                     /locus_tag="RHW33_003565"
     CDS             15871..16539
                     /locus_tag="RHW33_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV1647033.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNKYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(16557..17063)
                     /gene="tnpA"
                     /locus_tag="RHW33_003566"
                     /pseudo
     CDS             complement(16557..17063)
                     /gene="tnpA"
                     /locus_tag="RHW33_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            17071..>17486
                     /locus_tag="RHW33_003567"
     CDS             17071..>17486
                     /locus_tag="RHW33_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1647034.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYR"
BASE COUNT         4533 a         4034 c         4358 g         4561 t
ORIGIN      
        1 cagatgtttc ccatgaaaaa tggttaataa ctggacaaga taattttaaa attcagcaac
       61 ggttatcgat tatacatggt acttaaacag actcgcacca tttttgtaga aggttaaatt
      121 gagtacagtc tcatatatta agggtgttaa catggatgaa cgttttataa atatcacgcc
      181 gacttcgttg tcttttaaac acgccgatct gtcaggttta agctataaga gtgccaggct
      241 tgaacttacc ctgatatgcc cggcgatcaa gtcagaagtt aagatatcat ttgactggat
      301 acactcattt cgtgttactg acgaaggtga tttgttaggt atgtcaggaa agcagggaat
      361 taaccaaacg gggatctata gagtagccaa ttccacttat cttacatggt ttactatgca
      421 aagttgtgaa attcataaaa ataaaatgat cgaacattat gttattgcca cgtcaaatga
      481 tgttattgat gttttatcaa cagtttctcc gcaaatagtg tatgactaaa accagtgtct
      541 ggttcgcgat ggcgagatag aaaaagcatt atcctccttt cagagtgctc cgcttctcac
      601 tattatctca cgcagtattc ttaagggaac gataaggagg aaccatgaac attaccccgt
      661 ttccgacgct ttcgccggca accatagatg ccataaatgt tatcggacag tggctggcgc
      721 aggatgattt ctccggtgag gtgccgtatc aggctgattg cgtcatactt gcaggaaatg
      781 cggttatgcc gactatcgat gcggcatgta agattgctcg cgatcagcaa attcctttac
      841 tgattagtgg tggtatcggt cactcgacca cttttttgta tagcgccatc gcacagcatc
      901 cgcactacaa cactatccgc accactggca gagcagaagc gaccatcctg gcggatatcg
      961 ctcatcagtt ctggcatatt ccgcatgaaa aaatctggat tgaagaccag tcaacaaact
     1021 gcggtgaaaa cgcacgcttt agcatcgcgc tattgaatca ggccgtagaa cgagttcata
     1081 cggctatcgt tgtacaggac cccaccatgc agcggcgcac gatggcaacg ttccgccgta
     1141 tgaccgggga caatcccgat gcaccacgct ggttaagtta tcccggattc gttcctcagt
     1201 taggaaataa cgcagacagt gtaatcttta ttaatcagtt acaaggatta tggccggttg
     1261 agcgttatct ctcactactc actggcgagc tgccgcgttt acgcgatgat agcgatggct
     1321 acggtccccg cgggcgagat tttatcgttc acgttgattt tccggcagaa gtcattcagg
     1381 catggcaaac gcttaaacac gatgcggtgc tcatcgaggc gatggaaagt cgctcgttac
     1441 gttaaaaatt gcccgtttgt gaaccacttg tttgcaaacg ggcatgactc ctgactttta
     1501 tttctgcctt ttattccttt tacacttgtt tttatgaagc ccttcacaga attgtccttt
     1561 cacgatgccg tctctatgat gattgatgtt aattaacaat gtatttacca aaaacaaaca
     1621 tataaatcac aggagtcgcc catgtcagta cccgttcaac atcctatgta ttttgatgga
     1681 cagtttgtta cctggcgtgg agacgcatgg attgatgtgg taaaccctgc tacagaggct
     1741 gtcatttccc gcattcccga tggtcaggcc gaggatgccc gtaaggcaat cgatgcagca
     1801 gaacgtgcac aaccagaatg ggaagcgttg cctgcaattg aacgcgccag ttggttgcgc
     1861 aaaatctccg ccgggatccg cgaacgcgcc agtgaaatca gtgcgctgat tgttgaagaa
     1921 gggggcaaga tccagcagct ggctgaagtc gaagtggctt ttactgccga ctatatcgat
     1981 tacatggcgg agtgggcacg gcgttacgag ggcgagatta ttcaaagcga tcgtccagga
     2041 gaaaatattc ttctgtttaa acgtgcgctt ggtgtgacta ccggcattct gccgtggaac
     2101 ttcccgttct tcctcattgc ccgcaaaatg gctcccgctc ttttgactgg taataccatt
     2161 gtcattaaac ccagtgaatt tacgccaaac aatgcgattg cattcgccaa aatcgtcgat
     2221 gaaataggcc ttccgcgcgg cgtgtttaac cttgttctgg ggcgtggtga aaccgttggg
     2281 caagaactgg cgggtaaccc aaaggtcgca atggtcagta tgacaggcag cgtctctgca
     2341 ggtgagaaga tcatggcgac tgcggcgaaa aacatcacca aagtgtgcct ggaactgggg
     2401 ggtaaagcac cagctatcgt aatggacgat gccgatcttg aactggcagt caaagccatc
     2461 gttgattcac gtgtcattaa tagtgggcaa gtgtgtaact gtgcagaacg tgtttatgta
     2521 cagaaaggca tttatgatca gttcgtcaat cggctgggtg aagcgatgca ggcggttcaa
     2581 tttggtaacc ccgctgaacg caacgacatt gcgatggggc cgttgattaa cgccgcggcg
     2641 ctggaaaggg tcgagcaaaa agtggcgcgc gcagtagaag aaggggcgag agtggcgttg
     2701 ggtggcaaag cggtagaggg gaaaggatat tattatccgc cgacattgct gctggatgtt
     2761 cgccaggaaa tgtcgattat gcatgaggaa acctttggcc cggttctgcc ggtagtcgca
     2821 tttgacacgc tggaagatgc tatctcaatg gctaatgaca gtgattacgg cctgacctca
     2881 tcaatctata cccaaaatct gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt
     2941 gaaacttaca tcaaccgtga aaacttcgaa gctatgcaag gcttccacgc cggatggcgt
     3001 aaatccggta ttggcggcgc agatggtaaa catggcctgc atgaatatct gcagacccag
     3061 gtggtttatt tacagtctta atgagtgaaa gaggcggagg ttttttcctc cgcctgtgcg
     3121 cgtcagagtt tagcgaattt ttcgagggtg cgaataagct gcgtgacgaa gccatattcg
     3181 ttatcgtacc aggcgaccgt tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt
     3241 tgcgtggcat caaacaccga accgaaatgg ctgccaatga tatcggaaga gactatttct
     3301 tcatcggtat aaccaaatga ctcgttattg gtggttgctt gtttaagtgc gttattcacc
     3361 tcttcggcag tcactttttt tccgagaatg gacaccagct cagtgaccga acctgttttc
     3421 accggtacgc gttgcgcatg acctttcagt ttgccgctca gttccggtat caccagacca
     3481 atggcttttg ccgcccccgt agtgtgggga atgatatttt ctgccgcggc gcgtgaagca
     3541 cgtaaatctt taccacgcgg gccatccacc agtgactggg tgccagtata ggcatgaatg
     3601 gtcgtcatcg tgccgacttc tatcccgaaa ctgtcatgca aggctttggc catcggcgca
     3661 agacagttag tagtgcatga tgccacggaa acaatggtgt cgttgccatc cagagtgtcg
     3721 tcattgacgt tataaacgat agttttcatt tcaccggcag gggcggaaat caacaccttc
     3781 ttcgcgccag catcaagatg cgcctgcgat ttctcggcgg aggtataaaa gccagtacat
     3841 tcgacaatga tttctgcacc tttcgctttc cacggaatat ttttagcctc tttttcggcg
     3901 taaaccgcga tacttttccc attaacgata agtgaatctt ccgtaaaatc aacgctccag
     3961 gggaacggtc cgtagtttga atcatgtttc agcaggtagg cgagaatttt tggagaagtg
     4021 agatcattaa tagcgacaac gtctatgttg cttttgactt caagtaatcg acgcaacacc
     4081 agtcgaccga tacgaccaaa accgttaata ccaactttac tcatggtttt ctcctgtcag
     4141 gaacgttcgg atgaaaattg atcctttcca ggcttagacc aggatggcgg gatgggcaat
     4201 ctccattctt acagtgaaac gtaacgtact gaaaacgggt gaacaatatt taatgaaatt
     4261 ttgagaaaag cccgttatgt taacggaaaa ttatgttaaa gcaggaaatg ttatgaaaaa
     4321 taaatactca aggttacaaa tcagcattca ctggctggtc tttttgctgg ttatcgcagc
     4381 gtattgcgca atggagtttc ggggtttctt accacgtagc gatcggccac tcatcaacat
     4441 gattcatgtt tcctgcggca tatcaattct cgtgctgatg gtcgttcgtc ttttgttaag
     4501 gctgaaatac ccaaccccgc cgattatacc taagccaaaa ccgatgatga cgggactggc
     4561 gcatttggga catttggtga tttatctcct gtttattgcg ctgccagtga ttggtttggt
     4621 gatgatgtat aaccgaggca acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc
     4681 agaggccaat ttcgaactgg tagatagctt aaagtcgtgg catgaaacgc tggcgaatct
     4741 gggatatttt gtcatcggtt tgcacgctgc ggctgcactg gcacaccact atttctggaa
     4801 ggacaacaca cttctacgca tgatgccgcg taaacgttcc tgaaggatat ttaaagaaaa
     4861 cgcctgtact aaaaccgacc cgtggtacag gcgaagaata cgggtctaca tcggaagcgc
     4921 ctattatatt tatttgtatg acaaataaaa ccataatcct tgcccatacg tccatctggc
     4981 ttatttttaa tcaattcacc cgatctttga tctcatcaac ggtatcaaaa taaaccagcg
     5041 tataacgttc aaatcgaaca taagattcga taaaccatgg agtttatatg aaaaaactgg
     5101 cacttatttt gtttatggga acgcttgttt ccttttatgc cgatgccggg cgcaaaccct
     5161 gttctggttc gaaagggggg atctcacact gtacggcagg cggcaaattt gtctgtaatg
     5221 atggttctat tagtgcatcg aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat
     5281 gagaaaatga atctgctagt cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg
     5341 acgtgcaggc catgagcgca gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga
     5401 gcgtttgtcg ggtcaggagt gtgaatgtca gaatcgtaat gcagataatg agcagacaca
     5461 ccaccagagg gttgcgtttc atagcctttc tccttgccgg atggcgggta agaggctaag
     5521 atctgaattg ctatgttcat tcgttggcct cggttgatag aaatatcggt cggggccttc
     5581 gtctttctga ttcccggtta gcctgaaaac agaaagtctc aggcacccgt gagcatccta
     5641 tgtggtttcc ttagggacga aaataatcac ctgacgaaat tgcgtgctgt tttccagaat
     5701 ttttcgtcat tcgggttagc cagtttaacc attcgttact ctcttcattc caatggcatt
     5761 aatcttctct gcaataattg ttgtaaaaat gtgacgcaaa gaggctttta gtcgtaagta
     5821 attaccgtca aatgccgatg actttctatc aggagtaaac ctggacgaga gacaacggta
     5881 atgaatacaa ctccctcaca gcgattaggt tttttgcatc acatcaggtt ggttccgtta
     5941 tttgcctgca ttctaggcgg tatcttagtt ctattcgcat taagttcagc cctggctggc
     6001 tatttcctct ggcaggccga tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg
     6061 accgggttag cgaacagttc agattttttg cgttcagccc ggatcaatat gattcaggcc
     6121 ggggctgcga gtcgtattgc ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa
     6181 tcggagatta aacagtcgca gcaaggttat cgtgcttatc agaatcggcc ggtgaaaaca
     6241 cctgctgatg aagccctcga cactgaatta aatcaacgct ttcaggctta tatcacgggt
     6301 atgcaaccta tgttgaaata tgccaaaaat ggtatgtttg aagcaattat caatcatgaa
     6361 agtgagcaga tccgaacgct ggataatgct tataccgata ttttgaacaa agccgttaag
     6421 atacgtagca ccagagccaa ccaactggcg gaactggccc atcagcgcac ccgcgtgggt
     6481 gggatgttca tgattggcgc gtttgtgctt gccctggtca tgacgctgat aacatttatg
     6541 gtgctacggc agatcgtcat tcgtccactg caacatgccg cacaacggat tgaaaaaatc
     6601 gccagtggcg atctgacgat gaatgatgaa ccggcgggtc gcaatgaaat cggtcgctta
     6661 agtcgtcatt tacagcaaat gcagcattca ctggggatga cagtagggac tgttcgacag
     6721 ggcgcggaag agatttatcg tggcaccagc gaaatttccg ctggcaatgc ggacctttca
     6781 tctcgcaccg aagaacaagc ggcggctatc gaacaaactg ccgccagcat ggagcaactc
     6841 actgcgacgg tgaaacagaa tgcggataac gcgcatcatg ccagcaaact ggcgcaagag
     6901 gcttctatta aagccagcga tggcgggcag acggtttccg gggtagtaaa aacgatgggg
     6961 gctatctcta tgagttcgaa gaaaatttct gagatcaccg ccgtcatcaa cagtattgct
     7021 ttccagacga atattctggc actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa
     7081 gggcgtggat ttgccgttgt cgccagcgaa gtacggacac tcgcaagccg cagcgcccag
     7141 gcggcgaaag agattgaagg cttgatcagt gaatcagtca ggttaattga cctggggtcg
     7201 gatgaggtgg caacggccgg gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc
     7261 acacatatca tgcaggaaat cgccgccgcc tcggatgaac aaagtagagg cataacgcag
     7321 gttagccagg cgatttctga aatggataag gtgacgcaac agaatgcttc tctggtagaa
     7381 gaagcctcag cggcggcggt gtcccttgaa gaacaggctg cacgattaac tgaggcggtg
     7441 gacgtattcc gtctgaacaa acattctgtg tcggcagaac ctcgcggagc gggtgaacca
     7501 gttagtttcg ctacggtgtg aaaatgttca aggaagggat cgacagatcc cttcaccttt
     7561 cagaacggca ttgattttcg aatagcgtta atcatcaact ggcaaccaga agagaacgtc
     7621 gcatctacgc gggtcagtat tccaatcggt tcgcctgcac catgtcccgg tacaggcagg
     7681 gccaccagcg tggcatgacg caggtcgtct tttaccgcgc cagaagggac aaaccacacg
     7741 taatcgtatt caaccgtaag ttgacgagat agcgaagcag acagcgtttc gatacaaccc
     7801 gaaggaattt tacatccctg gctctgcact aatgcatctg aatgctggcg tggcgcagtg
     7861 ccttctggtg atacaacgac cggccattcc agcacccggc ttagcgttac gttctcctga
     7921 agtagcgggt gattagggcg gacaaccagc ttcagcgatt caagaaacag cagttcgtaa
     7981 ttaagcccgg tcatcagttc aggatctgac atccgaccaa tgccgatatc gatttctccg
     8041 gttttcaaac ccgccagaat catagggtta ctcattgtcg caacttgcaa ggtcgtctct
     8101 ttttgttgct gatgaaactg acctataacc gaaggtaata tccccagtgc cgcagtaggt
     8161 agtgcaccaa ccctgacgac atcattatta ggaccttctt tacgattaag cgactgtccg
     8221 gcagtgttga tggcgtcaag aactctgact gcatgcgtta aaaattgttc gccgggtaag
     8281 gtaagttgcg ccccctgacg accacgctca aacaagcgag cgcccgtcag ctgctccagt
     8341 tcattcaatg ttttagagag cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc
     8401 aaagttcctt gttgtgcgac agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga
     8461 ctaaacagac tatttttttc cataagcgat gttaaaaacg aagcggtgtc gctgacaagt
     8521 gaagttgttt gattatgata acttgattgc aaaatattat taacaattaa agcaattatg
     8581 ttacagcaaa atggataata ttgatgtttt cgcggcgaga tcacagtttg taaattcttc
     8641 ccgccagagt gaatgcggtt acctacactc cagattactg accactggag gcagacacta
     8701 tggcgaacag catcacggcg gatgagattc gggaacagtt ttcgcaggca atgtcagcca
     8761 tgtaccagca agaagttccg caatatggca cgctgctgga actggtagct gatgtgaatc
     8821 tggctgtgct ggaaaacaat cctcaactgc acgaaaaaat ggtaaatgca gacgagctgg
     8881 cgcgactgaa tgttgaacgt catggggcga ttcgcgttgg gactgcacaa gagcttgcta
     8941 ctcttcggcg gatgtttgcc attatgggga tgtacccggt gagctattac gatctctcgc
     9001 aggcaggggt gccggtacat tcgacagcat ttcggcccat tgatgatgct tctctggcgc
     9061 gtaatccctt ccgcgttttt acttcattac tccgccttga gcttatcgag aacgaaattt
     9121 tgcgccagaa agcggcggag attctgcgtc agcgcgatat cttcacccca cgttgtcgac
     9181 aactgttaga ggagtatgac cagcggggcg gttttaacga aacacaggca caggagtttg
     9241 tgcaggaggc cctggaaacg ttccgttggc accagtcagc aacggtagat gaagaaacct
     9301 atcgcgcctt gcacaacgaa catcggttga ttgctgatgt ggtctgtttt cctggatgcc
     9361 atatcaacca tctgacgcca cgtacgctgg atattgaccg ggtgcagtcg atgatgcctg
     9421 aatgcggaat tgaaccaaaa attctgatcg aagggccgcc gcgccgcgag gtaccgattt
     9481 tactacgcca gaccagcttt aaagcactgg aagagacggt gttgtttgcg gggcagaaac
     9541 agggcacgca tactgcgcgc tttggtgaaa ttgagcagcg tggcgtggca ttaacgccga
     9601 aaggtcgaca actgtatgat gatcttctgc gtaacgctgg aaccgggcag gataatctca
     9661 ctcaccaaat gcatttacag gaaaccttcc gcacttttcc tgacagtgag tttttaatgc
     9721 gtcagcaagg gctggcatgg ttccggtacc gtctgacgcc ttcaggtgag gcgcatcgtc
     9781 aggcgattca tcccggagac gatccacagc ccttaattga acgtggttgg gtcgcggcgc
     9841 aacccattac ctatgaagat ttcttgcccg ttagcgcggc ggggatcttc cagtcaaatc
     9901 tgggtaatga aacgcaggca cgcagccacg gtaatgccag tcgcgaagca tttgagcagg
     9961 cgttgggttg tccggttttg gatgagttcc agctttatca ggaagcggaa gaacgcagta
    10021 aacgtcgctg tggtttgctt taaattctga ccatccgtct ttgcaaaaat ttgcctgatt
    10081 ttacaaacga atcaggctca tcccatcgac ataaaaaaaa tgccgattta tgcatattct
    10141 ctcagttcaa caattggatt attaataaat attgtctaga gtgagcggtc ataaataagc
    10201 actttcttgc cgctgaaaac gaccagcgcg ggaccattca caacaccaga aggactcact
    10261 ttcaggtatg gatcgtagac gatttattaa aggttcaatg gctatggccg ccgtgtgcgg
    10321 taccagcggc attgcttctc ttttttctca ggcggcattc gcggcagatt ctgatattgc
    10381 cgacggacaa acccagcgtt ttgacttctc cattctacag tcaatggcgc atgacttagc
    10441 gcaaacagcg tggcgtggtg cgcctcgtcc gttacctgac actctggcga caatgacgcc
    10501 gcaggcttat aacagtattc aatacgacgc cgaaaaatcg ctctggcata acgttgagaa
    10561 ccgtcaactg gacgctcagt tcttccatat gggaatggga ttccgtcgcc gcgttcgtat
    10621 gttttctgta gatccagcaa cacatctggc gcgtgaaatt cactttcgcc cggagttgtt
    10681 caaatacaac gatgcaggtg ttgataccaa acaattagaa gggcaaagcg atctcggctt
    10741 tgccggtttt cgcgtgttta aagcccccga actggcgcgc cgtgatgtag tatcatttct
    10801 cggcgcgagt tatttccgcg ccgttgatga tacatatcaa tacggtttgt cggcccgcgg
    10861 cctggcgatt gacacttaca ccgacagtaa agaagagttc cccgacttta ccgccttctg
    10921 gtttgatacg gtaaaaccgg gggcaactac ctttaccgtt tatgcgttgc tcgatagcgc
    10981 cagcattact ggtgcctata agttcactat ccattgtgag aaaagtcagg tgattatgga
    11041 tgtggaaaat cacctgtatg cgcgcaaaga cattaaacag ctgggcattg cgccgatgac
    11101 cagtatgttc agctgcggta ctaatgaacg tcggatgtgc gacaccattc atccgcaaat
    11161 tcatgactct gatcgtctgt ccatgtggcg gggcaacggc gagtggattt gccgtccgct
    11221 gaataatccg caaaaattgc agttcaatgc ttacaccgac aacaacccga aagggtttgg
    11281 tttattgcaa ctggatcgtg atttctccca ttatcaggac attatgggct ggtataacaa
    11341 acgcccaagt ctgtgggtgg aaccgcgtaa caagtggggt aagggcacca tcggcctgat
    11401 ggaaatccca acaacgggcg aaacgctgga taacattgtc tgcttctggc agccagaaaa
    11461 agctgtaaag gcgggtgatg agtttgcatt ccagtatcgt ctgtactgga gtgcgcaacc
    11521 gcctgttcat tgcccattag cgcgcgttat ggcgacgcgt accggcatgg gcggtttccc
    11581 ggaaggttgg gcaccaggtg aacactatcc cgaaaaatgg gcgcgtcgtt ttgcagtcga
    11641 tttcgttggt ggtgatctga aagctgccgc gccaaaaggc attgagccgg tgattacgct
    11701 ttccagtggg gaagcgaagc aaatcgaaat tctctatatt gaacccattg atggttatcg
    11761 tattcagttt gactggtatc cgacttcgga ctccactgat ccggtcgata tgcggatgta
    11821 tctacgttgt cagggggacg ctatcagtga aacatggctg tatcagtatt tcccgccagc
    11881 gccggataaa cgtcagtatg ttgacgaccg cgtgatgagt taatcgtttt ttcttcggca
    11941 ccttcttcgg gaggtgccgt ctggttaaac acgatcccgc tcgcattttt ccctaagtta
    12001 aatgagtaat ctgatggtgt gtatttcaga tacaccttgt cagccactaa cagggagtgc
    12061 gtatgtttcc agaataccga gatttaatat cccgtctgaa aaacgaaaat cctcgcttta
    12121 tgtccttgtt cgataaacac aataaacttg atcatgaaat tgccagaaag gaaggttccg
    12181 acggtcgagg gtacaatgcg gaagtggtcc gcatgaaaaa acaaaagcta cagttaaaag
    12241 atgagatgct caaaatcctg cagcaggaga gcgtcaaaga ggtataaact ttcctaagcc
    12301 gccagccagg cggctttttt aacaactgca tggattgact ggagataaga tgactgaaac
    12361 gataaaagta agcgaatcac ttgaattaca tgctgttgca gaaaatcacg tcactcctct
    12421 ttatcagtta atctgtaaaa ataaaacctg gttacagcag tcgctaaact ggccgcagtt
    12481 cgttcagagt gaagaggaca cgcgaaaaac ggtgcagggt aatgtgatgt tgcatcaacg
    12541 cggctatgcc aaaatgttca tgattttcaa agaagatgaa cttatcggcg ttatctcgtt
    12601 taatcgtatt gaaccactga ataaaaccgc tgaaatcggc tactggctgg acgaatctca
    12661 tcaggggcag gggatcattt ctcaggcgct gcaggcattg attcatcatt acgcccagtc
    12721 tggtgaactt agacgcttcg tgatcaaatg tcgggtggac aatccgcaaa gcaaccaggt
    12781 cgctttgcgc aatggtttta tccttgaagg ttgcctgaaa caggctgagt tcctgaatga
    12841 tgcctatgat gatgtgaact tatacgcgcg tattatcgat tcacaataac cctgccagcg
    12901 gcgttcgcgt aatgcgctcc tcgccgttaa tgacttttgg accacggaca tgaaccgtgt
    12961 cgccatcaaa ggcttcaacg acggcgtggt cggttaactc cacatggttt tcaattatca
    13021 ccgcacctgt aatacggctc tcaccttgta ttacaacgtg ctcgtcgagc agaatcggtc
    13081 caccacgtac cacggcattt ccgccaatca agacatgatg ttttaacaca caattacctt
    13141 ccacaatggc atattccgcc acttgcgaac tgtaatgaat cgtcggaatg gcatcttctt
    13201 ctatgccagc tttcacctgc gcgtgaccgt agactttagc gcaatcgcat agccagacat
    13261 tgttctcttc attaccttca atactggcaa aatcaaaaac ttcggcgcga tgttcaataa
    13321 aagcataccg gatgacggca tcgccataaa tttgtgcctg gtggacaata cgcgaggcgc
    13381 taacccttgc gcgatcataa atttgtagca ggagttgatg gtcaggcgtt aagccttgtg
    13441 ctgcgacgat catagaatgt tgattaatta aggcgtgacc aaatattcta cattgcccgc
    13501 aaaccagtga gtcatgaatg gtgacactgt cacttatata tgcaccctgg ctgatttcac
    13561 tattatcgat ccagacgtta tccgttgcgt aaacctctcc ccacagcacg ctggtgccgg
    13621 tgatacgcgt attaccggaa atcaccgttc cgccgaacgc aatagcattc tggtcgtaaa
    13681 tccagcaatt tccttcctgt gctaacactg tttcgcgatc gatccagccg ccagcggttc
    13741 ctgctatcac atcgttaaaa tcgctaatgg cgataatctg ccgtaataac acactttttt
    13801 tagtgccatc ttcctgataa ctaaaggctc gctgttcttc acttagacga tatttgcgca
    13861 tagcgttttc ccacaggtga cttactataa ccgtagcaaa ttctgcggct ctggctatgc
    13921 tcgagaaaat tccataaaat gcatttcaaa tatactttat aaattaaaca aaatgagtaa
    13981 gaagatgcag agcgataaag tgctcaattt gccggcaggc tactttggta ttgtgttggg
    14041 gacgataggg atgggatttg cctggcgcta tgccagtcag gtttggcagg tcagccactg
    14101 gttaggggat gggctggtga ttctggcgat gatcatctgg ggattattga ctagcgcatt
    14161 tattacccga ctcatacgct ttccgcatag cgtgctggcg gaagttcgcc atccagtgct
    14221 gagcagtttt gtgagtttgt ttccggcaac gacgatgctg gtggcgattg gttttgttcc
    14281 gtggtttcgc ccactggcgg tgtgcctgtt cagttttggt gtcgtggttc agttggctta
    14341 tgccgcctgg caaactgcgg gattatggcg cggatctcac cctgaagaag ctaccacgcc
    14401 tggactgtat ctgccgacag ttgccaacaa ctttatcagc gcaatggcct gtggtgcgtt
    14461 gggctacacc gacgccggtc tggtgttttt aggcgcaggc gttttctcat ggctaagcct
    14521 tgaaccggtg atcttgcagc gtctgcgcag ttcgggagaa ttacccacgg cactgcggac
    14581 atctctcggc attcaactcg ctcctgcgct ggtggcctgt agtgcctggc tgagcgtcaa
    14641 cggcggcgag ggtgacacgc tggcgaaaat gcttttcggt tatggactgc tgcaactgct
    14701 gtttatgcta cgtctgatgc catggtatct ctcccagcca tttaatgctt cattctggag
    14761 tttctcgttc ggtgtatctg cactggcaac caccggtttg catctgggga gtggcagcga
    14821 taatggattt ttccatacgc tggcggtgcc gctgtttatc tttaccaatt ttattattgc
    14881 aatactgctc atccgtactt ttgcgcttct gatgcaggga aaattgttag tcagaaccga
    14941 gcgcgccgtt ttaatgaaag cagaggacaa agaatgatta ttcgtgacga aaactatttt
    15001 actgataaat atgaattaac ccgcacacac tctgaagtac tggaagcggt gaaagtggtt
    15061 aaaccgggta aaacgctgga tctgggctgt ggcaatggtc gtaacagtct ttacctggca
    15121 gccaatggtt atgatgttga cgcatgggat aaaaatgcca tgagtatcgc caacgtcgag
    15181 cgcattaaat ctattgaaaa tctggataat ttacacactc gggtcgttga tctgaataac
    15241 ctcacatttg ctggacagta cgattttatt ctttcgactg tggtgctgat gttccttgag
    15301 gctaaaacca tcccagggct gattgccaat atgcaacgtt gcactaaacc tggcggttac
    15361 aacctgattg tggcggcgat ggataccgct gattatccat gtaccgtcgg cttcccgttt
    15421 gccttcaaag agggagaatt acgtcgatat tacgaaggct gggagatggt gaaatacaat
    15481 gaagacgtcg gcgagctgca ccgcaccgac gccaacggta atcgtattaa actgcgtttc
    15541 gccacgatgc tggcacgtaa aaaatgaccc ggtaagaaca aagcgcgtga aaattcccca
    15601 cgctgagatg gtttactgtc cttcttttcg gtaagcatat tttttatcga agggatgtga
    15661 aattaatcac ggtagtcgaa gttttttagc agcttaactt actgaaattt aactactgat
    15721 gattgactta gccccttttt cggcattgac tatgtcgtct gaaaaggggc taaaaaaatt
    15781 tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct ataacttaaa
    15841 aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc gaaagttgca
    15901 gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca gccagataaa
    15961 tattctggtt ttttaaacaa atactctgat ttaaaagaaa caacctcggc tacaggtaaa
    16021 cctgttttac gttgggtaga cccgagtttt gatcaaagca aatatgacag catcgtctgg
    16081 aacccaatca cttattatcc ggtaccaaaa ccttctaccc aggtagggca gaaagttctg
    16141 gataaaattt tgaactatac caacaccgaa atgaaagaag cgatagcgca gcgtaaacca
    16201 gtggttacca ccgctgggcc gcgtagtctg attttccgtg gggccattac cggtgtagat
    16261 accagcaaag aagggctgca attctatgaa gtggttcctg ttgcattagt ggttgcgggg
    16321 acgcaaatgg ctacaggcca ccgtaccatg gatactcgct tgtattttga aggtgagctg
    16381 attgatgcag cgactaataa accggttatc aaagtcgttc gtcagggcga aggtaaagac
    16441 ctgaataacg aaagtacgcc aatggctttc gaaaatatta aacaagttat tgatgacatg
    16501 gcgaccgatg ccaccatgtt tgacgttaac aaaaagtagt tccagacgcg ccatcgttag
    16561 atggcgcttt ttatccggtg cgccgtaaaa ccccatcctt cagggcgggg atataaggcg
    16621 cggttttcca cctgaccagg tgtttgctgt tgttcaatgt attggcggat gacggatatt
    16681 ggcgcacctc cgcagctact ggcaaaatag ccaggactcc acaaaacgcc tttgtagtaa
    16741 tacctgactg caatatctgg tcgatctcgt cgcagtaacc tacccgatac gcctttgagg
    16801 ctgtttacca gactggatat cgccagtttg ggaggttagt taattaacaa atggacgtga
    16861 tctggttcgc catccatttc aaccagttca gcttcaaaat aagcacatac atttgaaaag
    16921 taagtgcgta gtttttctgt cgcgtcatgg tcaaaaatct ggcgtcggta tctggtgaca
    16981 aagaccaggt gaacatgcat caggaaaaca caatgccttc cacgccggat atcggtttct
    17041 tttttcacag accaaagtat catttgacct gtgaaacgat tacaggcatt taaattccag
    17101 ttaagaccag gtggtcaaca ggagtgtgaa atgaggcgct tcgcaggcgc atgtcgtttc
    17161 gttttcaatc gtgctctggc acgtcagaat gagaatcatg aggccggtaa taaatacatc
    17221 ccttacggga aaatggcttc ctggctggtt gagtggaaaa atgccactga aacgcaatgg
    17281 cttaaagatt ctccctcaca gccattgcaa cagtcactga aagaccttga gcgggcttac
    17341 aaaaacttct tccggaagcg ggctgctttt ccccgattca aaaagcgggg acagaatgat
    17401 gcattccgct acccgcaggg cgttaagctc gatcaggaaa acagccgtat ttttctgccg
    17461 aaactgggct ggatgcgcta ccggaa
//
LOCUS       DAPGRN010000102        17462 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPGRN010000102 DAPGRN010000000
VERSION     DAPGRN010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.103"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            473..1801
                     /locus_tag="RHW33_003568"
     CDS             473..1801
                     /locus_tag="RHW33_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647035.1"
                     /translation="MRPLSNLNTSAATYIPPQQLPSSCLETLTLLVQMNNYIKRDADY
                     STGMAVTPLVPEEVHLLAVAMTTELHRHQFQPVVSANDLQLPEPFTFDVAGFTITFTK
                     TQEHDNTGNLMKIAVSKSGVCTSTNITLELFHSIVTTLMSRSQYGTFDLWSVRPILTD
                     ENQNRVHEAARYSPAQQYGREETHFTNNGMREFGNMSPLAWRNDVELQQSCNTSNIPP
                     NTANDDINNTRQTIEDEPQQYVKLTVDDMRKWAAMDQQARNALQGVPGWCTRNHFNIK
                     NARNYLTDHGLNYAGQVKVNRPHEYAKFTLEHIRQWAALNKHVRKPVGYLEKWCKERN
                     LAPTTARNYLKNDGLTALGELKLTGPQKWVTFTFGDIVQWANMTQEERNSAGGAEKWS
                     KKRGFQWSTARSYLKSSGVTKQGARKLAWLKNSGNMSNPFYLAQPTSRRT"
     gene            complement(1950..3458)
                     /gene="putP"
                     /locus_tag="RHW33_003569"
     CDS             complement(1950..3458)
                     /gene="putP"
                     /locus_tag="RHW33_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV1647036.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(3617..3823)
                     /locus_tag="RHW33_003570"
     CDS             complement(3617..3823)
                     /locus_tag="RHW33_003570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647037.1"
                     /translation="MLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQL
                     TFLKGATAKNVRECNLMKNSVAEH"
     gene            3881..7843
                     /gene="putA"
                     /locus_tag="RHW33_003571"
     CDS             3881..7843
                     /gene="putA"
                     /locus_tag="RHW33_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV1647038.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANEGDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPC
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPSGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEVADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QIMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(7882..8520)
                     /gene="rutR"
                     /locus_tag="RHW33_003572"
     CDS             complement(7882..8520)
                     /gene="rutR"
                     /locus_tag="RHW33_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV1647039.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            8808..9899
                     /gene="rutA"
                     /locus_tag="RHW33_003573"
     CDS             8808..9899
                     /gene="rutA"
                     /locus_tag="RHW33_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV1647040.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPVLTQEVA"
     gene            9899..10591
                     /gene="rutB"
                     /locus_tag="RHW33_003574"
     CDS             9899..10591
                     /gene="rutB"
                     /locus_tag="RHW33_003574"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV1647041.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            10603..10989
                     /gene="rutC"
                     /locus_tag="RHW33_003575"
     CDS             10603..10989
                     /gene="rutC"
                     /locus_tag="RHW33_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV1647042.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINETYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            10997..11797
                     /gene="rutD"
                     /locus_tag="RHW33_003576"
     CDS             10997..11797
                     /gene="rutD"
                     /locus_tag="RHW33_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV1647043.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAAHAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHAVRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            11807..12397
                     /locus_tag="RHW33_003577"
     CDS             11807..12397
                     /locus_tag="RHW33_003577"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV1647044.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARIWFTSSPQLAEETAFRNSSMQAAYLIIACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            12408..12902
                     /gene="rutF"
                     /locus_tag="RHW33_003578"
     CDS             12408..12902
                     /gene="rutF"
                     /locus_tag="RHW33_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV1647045.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            12923..14251
                     /gene="rutG"
                     /locus_tag="RHW33_003579"
     CDS             12923..14251
                     /gene="rutG"
                     /locus_tag="RHW33_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV1647046.1"
                     /translation="MAMFGLPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASGFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(14334..14507)
                     /locus_tag="RHW33_003580"
     CDS             complement(14334..14507)
                     /locus_tag="RHW33_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV1647047.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            14880..15476
                     /gene="wrbA"
                     /locus_tag="RHW33_003581"
     CDS             14880..15476
                     /gene="wrbA"
                     /locus_tag="RHW33_003581"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV1647048.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            15497..15724
                     /locus_tag="RHW33_003582"
     CDS             15497..15724
                     /locus_tag="RHW33_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV1647049.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(15762..17003)
                     /gene="agp"
                     /locus_tag="RHW33_003583"
     CDS             complement(15762..17003)
                     /gene="agp"
                     /locus_tag="RHW33_003583"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV1647050.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDADGFCPMDKFDSVLNEAVK"
BASE COUNT         4181 a         4608 c         4572 g         4101 t
ORIGIN      
        1 agaaagagaa gtaataagat gagacgccac tgctttcaga tgaaccctgt aattagggtc
       61 gagaatatag tttatccatt cccctatcga taaattaaaa aatttgcgtg ttaatttttc
      121 tctgactgaa ggctaacgtc gaattaccga caaaattcac gagtgattgt aactacgtac
      181 tttgtgaaac acaggaagaa attattattt accgcgatga ttcaaggcgt aaaaaaaata
      241 ataatcttca tgaaactcac cccgatggca gcattgtaaa ttcgccaata actggcattc
      301 ctcctgcttt ttacgggtgt ttccattaac accagaatga gcggattgtt tactacatca
      361 agaacgcatc cgaagtgtac atcctggtgc gcagactgaa taacccggag ccatgcggat
      421 ggctacgact taatacgaat cattaaccct gtaaggatac ggagagcatg acatgagacc
      481 actatccaac ctcaatacga gcgcagcaac ctatattcca ccgcagcaac tcccgagtag
      541 ttgtctggaa actttgacgc ttctggtgca gatgaataat tatatcaaaa gggatgcgga
      601 ctacagcacg ggcatggctg ttactccact ggttccagaa gaggttcact tgctggctgt
      661 tgccatgacg accgaactgc atcgacatca gttccaaccg gtggtaagtg caaatgatct
      721 gcaacttcct gaaccgttca ccttcgatgt tgccggtttc accatcacct tcacgaagac
      781 gcaagagcat gacaacacgg gcaatctcat gaaaattgca gtcagcaaga gcggtgtgtg
      841 caccagtaca aatattactc ttgagctgtt tcactccatt gtcacaaccc tgatgtcacg
      901 cagtcaatat ggaacctttg atctctggtc cgtacggcca atactcacgg atgaaaatca
      961 aaacagggtg catgaagcag ctcgctactc cccggcccag caatacggaa gagaagaaac
     1021 acactttacc aacaatggca tgcgagaatt tggaaatatg tctccgctgg catggcgtaa
     1081 cgatgtggaa ttacagcaat cgtgtaatac aagcaacatc ccaccaaata ctgcaaatga
     1141 cgacataaac aacaccaggc agaccatcga ggacgagcca cagcaatatg ttaaattgac
     1201 tgtagacgac atgcgaaaat gggcggctat ggaccagcag gcgcgaaacg ctctacaggg
     1261 agttccagga tggtgtacgc gcaatcattt taatatcaaa aatgccagaa attacctgac
     1321 cgatcacggc ctgaactacg cgggacaggt aaaggtaaac aggccgcatg aatatgccaa
     1381 attcaccctt gaacatatcc gccagtgggc agccttaaac aagcatgtgc gaaaaccggt
     1441 tggttattta gaaaagtggt gtaaggaaag aaacctcgcg ccaactactg ccaggaatta
     1501 cctgaagaac gacgggctta ccgccctggg tgagttgaag ctgacagggc cgcagaaatg
     1561 ggtcacgttc accttcggag acatcgtaca atgggcgaat atgacccagg aggagcgtaa
     1621 cagcgcaggg ggagccgaaa agtggagtaa aaaacgcggc ttccagtggt ctactgccag
     1681 atcctacctg aaatcgagcg gagtgaccaa gcaaggtgcg agaaaactgg cgtggttaaa
     1741 aaacagcggg aatatgtcga atccattcta tctcgcccaa ccaaccagcc gcaggacata
     1801 agctgaaggt accgatagcg gcaattttgt tcatctgtgc cggctgggca aatagcaatt
     1861 gcccccgaac actccctgcc aggcaactta taccgcatgg cagggaacat tcgggttaaa
     1921 aagcaaaaga taaataatta ctcttcagct tagccttcct gcaaccgtga cggcggagcc
     1981 gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga cggcgcttta
     2041 cccagcaaac taaacactac aatcccaata ctgccaaaga taaagcccgg aataatttcg
     2101 tacagtccca gccagccgaa ctgtttccag acgataaccg tcagcgcccc gatgatcatc
     2161 cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaatag caccaccgga
     2221 ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac gcggttttcc
     2281 gggttcgctg ccagcgcaat ggccaccagc gccaccacca gcaccatcac acgccctacc
     2341 cacaccagtt ctttctggct ggcatgttta cgcagaaacg ctttgtacaa atcttcggta
     2401 atcgcactgg aacataccag cagctggcaa cttaacgttg acattaccgc cgccagaatt
     2461 gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag ttcgataaac
     2521 acgcgttcag cgttctggtt taccgcacct gctaccgccg ggtgctcgtt aaagtaagcg
     2581 ataccaaaga agccgacagc caccgcccct gccaggcaga ggatcatcca ggtcatactg
     2641 atacgacgcg catggacgat gctgtggtga gaatccgccg ccataaaacg cgccaggatg
     2701 tgcggttgcc cgaagtaacc cagcccccac cccatcagcg agataatggc gacaaagttc
     2761 agccctttga gcatatccac gttttcgatg ctcttttgtt tgatcacttc cagcgagtca
     2821 ccaaagccac cgacactgat aatgacgata accggcgtca ggatcagggc aaaaatcatc
     2881 aggctggcct gtacagtgtc agtccagctc accgcgagga aaccgccaat aaaggtgtaa
     2941 aggatcgtcg ccgcagctcc cgcccacaga gccgtttcgt agctcatgcc aaaggtactt
     3001 tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa gaacagcaaa
     3061 ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaaacg cccggtgaaa
     3121 taatccggca atgtcaaggc gttattgttg tattcggtat gcacacgcaa ccgcccggcc
     3181 accagcttcc agttaatcca cgcgcctaat gtcaggccaa tggcgatcca actttcggaa
     3241 atcccggaaa gaaaaaccgc gcccggcaac cccattaaca gccagccgct catatccgat
     3301 gcacccgccg ataatgccgt cacgaatggc ccaaggctac gtccgcccag aatatagtcg
     3361 tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc aaagatatag
     3421 acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca aaaaattatt
     3481 atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac agagtttatg
     3541 ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg atttaacaca
     3601 ccatttacat taaattttag tgctcagcga cactattttt catcaggttg cactctctca
     3661 cattttttgc ggttgcacct ttcaaaaatg ttaactgccg cagagaaaaa gtctgagtta
     3721 tttttttaat ccctgtcata tcgatttctt ttattaacat ttcattcatt tttaagcttg
     3781 ctacgcaggt cacatttaac atggttgcac aaagttgcaa cataatggat atttcacgat
     3841 aacgttaagt tgcacctttc agaacaacag gagtaatggc atgggaacca ccaccatggg
     3901 ggttaagctg gacgacgcga cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg
     3961 cacgccacac tggttaatta agcaggcgat tttttcttat cttgaacaac tggaaaacag
     4021 cgatactctg ccggagctac ctgcgctgct ttctggcgct gccaatgagg gcgatgaagc
     4081 accgactccg gcagaggaac cacaccagcc attcctcgac tttgccgagc aaattttgcc
     4141 ccagtcggtt tcccgcgccg cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt
     4201 ttctatgctg ctggaacaag cccgcctgcc gcagccagtt gctgaacagg cacacaaact
     4261 ggcgtatcaa ctggccgata aactgcgtaa tcaaaaaaat gccagtggcc gcgcaggtat
     4321 ggtccagggg ttattgcagg agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg
     4381 tctggcagaa gcgttgttgc gtattcccga caaagccacc cgtgatgcgt taattcgcga
     4441 caaaatcagc aacggtaact ggcagtcaca cattggtcgt agcccgtccc tgtttgttaa
     4501 tgccgctacc tgggggctgt tgtttactgg caaactggtt tccacccata acgaagccag
     4561 cctctcccgc tcgctgaacc gcattatcgg taagagcggt gaaccgttga tccgcaaagg
     4621 tgtggatatg gcgatgcgcc tgatgggaga gcagttcgtc acgggcgaaa ccattgcaga
     4681 agcgttagcc aatgcccgca agctggaaga gaaaggtttc cgttattctt acgatatgct
     4741 tggcgaagct gcactaactg ccgcagatgc acaagcgtat atggtttcct atcagcaggc
     4801 gattcacgcc attggtaaag cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc
     4861 aatcaagctg tcggcgctgc atccgcgtta cagccgcgcc cagtatgacc gggtaatgga
     4921 agagctttac ccacgtctga aatcactcac cctgctggcg cgtcagtacg atattggtat
     4981 caacattgac gccgaagagg ccgatcgtct ggagatctcc ctcgatctgc tggaaaaact
     5041 ctgtttcgag cctgagctgg caggctggaa cggcatcggc tttgttattc aggcttatca
     5101 aaaacgctgt ccgttggtga ttgattacct gattgatctc gccacccgca gccgtcgccg
     5161 tctgatgatt cgcctggtga aaggcgcgta ctgggatagt gaaatcaagc gtgcacagat
     5221 ggacggcctt gaaggttatc cggtttatac ccgtaaggtg tataccgacg tttcttatct
     5281 cgcctgtgcg aaaaagctgc tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca
     5341 caacgcccat acgctggcag cgatttatca actggcaggt caaaactact atccgggtca
     5401 gtacgagttc cagtgcctgc atggtatggg cgagccactg tatgagcagg tcaccggaaa
     5461 agttgccgac ggcaaactta accgtccgtg tcgtatttat gccccggttg gcacacatga
     5521 aacgctgctg gcgtatctgg tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt
     5581 taaccgtatt gccgacacct ctttgccact ggatgaactg gtcgccgatc cggtcactgc
     5641 tgtagaaaaa ctggcgcaac aggaagggca aactggatta ccgcatccga aaattcccct
     5701 gccgcgcgat ctttacggtc acgggcgcga caactcggca gggctggatc tcgctaacga
     5761 acaccgtctg gcctcgctct cctctgccct gctcaatagt gcactgcaaa aatggcaggc
     5821 cttgccaatg ctggaacaac cggtagcggc tggtgaaatg tcacccgtta ttaacccggc
     5881 agagccgaaa gatattgtgg gctatgtgcg tgaagccacg ccgtgtgaag tagaacaggc
     5941 gctggaaagt gcggttaata atgcgccaat ctggtttgcc acgcctccgg ctgaacgcgc
     6001 ggcgattttg catcgcgctg ccgtgctgat ggaaagccag atgcagcaac tgattggtat
     6061 tctggtgcgt gaagccggaa aaaccttcag taacgccatt gccgaagtgc gcgaagcggt
     6121 cgattttctc cactactacg ccggacaggt gcgggatgat ttcgctaacg aaacccatcg
     6181 tccattaggg cctgtggtat gtatcagccc gtggaacttc ccgctggcga ttttcaccgg
     6241 gcagatcgct gccgcactgg cggcaggtaa cagcgtactg gcaaaaccgg cagaacaaac
     6301 gccactgatt gccgcgcaag ggatcgccat tttgctggaa gcgggcgtac cgtcaggcgt
     6361 ggtgcaattg ctgccaggtc agggggaaac cgtgggcgcg caactgacgg gtgatgatcg
     6421 cgtgcgcggg gttatgttta ccggttcaac cgaagtcgct acgttgctgc aacgcaatat
     6481 cgccagccgc ctggacgctc agggtcgccc tattccgctc atcgctgaaa ctggcggtat
     6541 gaacgcgatg attgtcgatt cctccgccct gaccgaacaa gtcgtaatag atgtgctggc
     6601 ctcggcgttc gacagtgcgg gtcagcgttg ttcggcgctg cgcgtgctgt gcctgcaaga
     6661 tgaggttgcc gaccacacgt tgaaaatgct gcgcggcgca atggccgaat gccggatggg
     6721 taatccgggt cgcctgacca ccgatatcgg tccggtgatt gatagcgaag cgaaagccaa
     6781 tatcgagcgt catattcaga tcatgcgtag taaaggccgt ccagtgttcc aggcggtgcg
     6841 ggaaaacagc gaagatgccc gtgaatggca aagcggcacc tttgtcgccc cgacgctgat
     6901 cgaactggat gactttgccg aattgcaaaa agaggtcttt ggtccggtgc tgcatgtggt
     6961 gcgttacaac cgtaaccagc tgccggagct gatcgaacag attaacgctt ccggctatgg
     7021 tctgacgctt ggtgttcata cacgtattga tgaaaccatc gcccaggtca ctggctcagc
     7081 ccatgttggt aacctgtatg ttaaccgtaa tatggtgggc gcagtggtgg gtgtgcagcc
     7141 attcggcggc gaaggattgt ccggcaccgg gccaaaagca ggcggtccgc tctatctcta
     7201 ccgtctactg gcgaatcgcc cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc
     7261 agagtatccg gtcgatgcgc agttgaaagc cgcattgact cagccgctaa atgcactgcg
     7321 ggaatgggca gcaaatcgtc cagaattgca ggcgttatgt acgcaatatg gcgagctggc
     7381 gcaggcagga acacaacgat tgctgccagg gccgacgggt gagcgcaaca cctggacgct
     7441 gctgccgcgt gagcgcgtat tgtgtatcgc ggatgatgaa caggatgcgc tgactcagct
     7501 cgccgccgtg ctggcggtgg gcagccaggt gctgtggcca gatgacgcgc tgcatcgtca
     7561 gttagtgaag gcattgccat cgactgtcag cgagcgtatt caactggcga aagcggaaaa
     7621 tattaccgct caaccgtttg atgcggtgat cttccacggt gattcggatc agcttcgcgc
     7681 attgtgtgaa gcagttgccg cgcgggatgg cgcaattgtt tcggtgcagg gttttgcccg
     7741 tggcgaaagc aatatccttc tggaacggct gtatatcgag cgttcgctga gtgtgaatac
     7801 cgctgccgct ggcggtaacg ccagtttaat gacgatcggt taacaggtag ccggaggatg
     7861 ttacaccatc ctccggcatc attagcgtgg tcgaattccc tcaataataa tccgctgcac
     7921 gttttcaacc gtttgattga aaaatacctc atcgcgcaac gttgcgcctg tcactgcctc
     7981 cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc caaatcataa aaatcaaatg
     8041 ctgcggatca attggcgcga gtttgccgct tttgacccaa ccggcaatca gcgccgattt
     8101 ctcatcaatt aatgccttca aatcgcccgt cagttcatcc attaacagcg gcgcgcctgc
     8161 cagcatctcc atacagaaca ggcgcgaagc ctgcggataa tcgcgtgaga cttccagctt
     8221 cagacggatg tactctttga tcgccgccag cggggcgaaa tcttcacgaa acgcttttaa
     8281 cggtgccagc cagatatcga gaatctgccg cagcacggca atatacagcg cctcttttga
     8341 cggaaagtaa tacagcagat tggtttttga aacacccgcc aactctgcga tctgctccag
     8401 ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt
     8461 tttcttcgcg cttactgcac gcgaacgttt acccgttgtt ttgactgcgc cttgcgtcat
     8521 gcgctctccc ctcttgtaga ttggcaacag gatagcaaac cccccgcccg gcctcgacct
     8581 tctgcactct catcgcgcac agatgcatgt tttatgtgca actgttttga ccgtttagtc
     8641 cactttttac cagacatttt agataacgca atgttaacac attaataaca ctcccttttt
     8701 aaaaactggc atccgctttg caaacaagcc aatacgcggc tgaaaaggat atgcaggatg
     8761 cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg
     8821 tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc
     8881 cgacctttga actgaataaa gccatcgtgc aaaaagcgga gcactaccat ttcgatttcg
     8941 ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc
     9001 ttgagtcgtt caccttgatg gcggggctgg cggccgtgac ctcgcgcatt cagatttacg
     9061 ccaccgccgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg
     9121 actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgagt
     9181 atgagcagat gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca
     9241 ccgagtatgt tcaggtgctg cgcgacctgt ggggcacggg aaaaagcgat tttaaaggcg
     9301 attttttcac catgaatgat tgtcgcgtca gtccgcaacc gagtgtcccc atgaaagtga
     9361 tctgcgccgg gcaaagcgac gctggcatgg cgttctccgc ccagtatgcc gatttcaact
     9421 tctgtttcgg caaaggcgta aatacaccca cggctttcgc cccgaccgct gcgcggatga
     9481 aacaggccgc agagcaaacc gggcgcgacg ttggctctta tgtattgttt atggtgattg
     9541 ccgatgaaac cgacgatgcc gctcgcgcca aatgggaaca ctacaaagcg ggcgcggatg
     9601 aagaggcgtt aagctggcta accgaacaaa gtcagaaaga tacccgctcc ggtactgaca
     9661 ccaacgtccg tcagatggcc gatcccactt cggcggtaaa catcaatatg gggacgttag
     9721 tcggttctta cgccagtgtc gcgcgcatgt tagatgaagt cgcaagcgtg cctggtgccg
     9781 aaggcgtgct gttaaccttc gacgattttc tgtcgggaat cgaaaccttc ggcgagcgca
     9841 ttcaaccact gatgcagtgc cgcgcccatc tccctgtgct gactcaggag gtggcatgat
     9901 gactacatta accgctcgac cggaagccat taccttcgat ccgcagcaaa gtgcgctgat
     9961 cgtggtggat atgcaaaacg cctatgccac gccaggcggc tacttagatc tcgccgggtt
    10021 tgatgtctca accactcgcc cggtcattgc caacattcaa accgccgtga ccgcagcgcg
    10081 agcggcaggg atgttgatca tctggtttca aaatggctgg gatgaacagt atgtcgaggc
    10141 tggcggcccc ggctcaccga attttcataa atcgaacgcc ctgaaaacca tgcgtaagca
    10201 gccgcagctg caggggaaat tgctggcgaa aggctcctgg gattatcaac tggtggatga
    10261 actggtgccg cagcctggcg atattgtgct gccgaagccg cgctacagcg gtttcttcaa
    10321 tacgccgttg gacagcattt tgcgcagccg tggaatacgc catctggttt tcaccggtat
    10381 cgctaccaac gtctgcgtcg aatcgacgct acgcgacggc ttttttctgg agtatttcgg
    10441 cgtggtgctg gaagacgcaa cacaccaggc ggggccggaa tttgtacaga aagccgcgtt
    10501 gttcaatatc gaaacctttt ttggctgggt cagcgacgtc gaaacgttct gcgacgcgct
    10561 ttctcccacg tcctttgctc gtatcgctta aggagtttaa cgatgccaaa atccgtaatt
    10621 attcccgctg gcagcagcgc accgctggcc cccttcgttc ccggcacgct ggctgatggc
    10681 gtggtgtatg tctccggtac gctggctttt gatcaacata ataacgtgct gtttgccgat
    10741 gacccaaagg cgcaaacccg ccacgttctg gaaactatcc gcaaggtgat cgagacggcg
    10801 ggtggcacga tggcggatgt gaccttcaac agcatcttta ttaccgactg gaaaaattac
    10861 gccgcgatta acgaaaccta cgccgagttt tttccaggtg ataaaccggc gcgattctgc
    10921 attcagtgcg gactggtaaa acctgacgcg ctggtggaaa tcgccacaat tgcgcatatc
    10981 gccaagtgag gccgcgatga aactttcact ctcacctccc ccttatgctg atgcgcccgt
    11041 agtggtgttg atttcgggtc ttggcggtag cggcagttac tggttaccgc aactggcggt
    11101 gctggagcag gagtatcagg tagtctgtta cgaccagcgc ggcaccggca ataatcccga
    11161 cacgctggca gaagattaca gtatcaccca gatggcagcg gaactgcatc aggcgctggt
    11221 agccgcaggg attgagcatt acgcagtggt cggccatgcg ctcggtgcgc tggtgggaat
    11281 gcagctggcg ctggattatc ccgcgtcggt aactgtgctg gtcagcgtta acggctggct
    11341 acgaataaac gcccatacgc gccgctgttt tcaggttcgc gaacgattac tgtatagcgg
    11401 tggcgcgcag gcatgggtgg aagcgcagcc gttgttcctc tatcccgccg actggatggc
    11461 ggcccacgca cctcgccttg aggcagaaga cgcactggca ctggcgcatt ttcagggcaa
    11521 aaataattta ctgcgtcgac ttaacgccct caaacgcgct gactttagtc atcatgcggt
    11581 tcgcatccgc tgcccggtgc aaatcatctg cgccagtgat gatctgctgg tgccatcagc
    11641 atgttccagt gaacttcatg ccgccctgcc cgatagccag aaaatggtga tgcgctatgg
    11701 cggacacgcc tgcaacgtga ccgatcccga aacgtttaat gctctgttac tcaacgggct
    11761 tgccagcctg ttacatcacc gtgaagccgc cctgtaagga attgctatga acgaagccgt
    11821 tagcccaggt gcgcttagca ccctgttcac cgatgcccgc actcacaacg gctggcggga
    11881 gacacccgtc agcgatgaga cgttacggga gctttatgcc ctgatgaaat gggggccgac
    11941 atcagctaac tgttctccgg cacgtatcgt gtttatccgc actgcagaag gaaaagaacg
    12001 tctgcgcccg gcgctttcca gcggcaatct gcaaaaaacc ctgaccgcgc ccgtcaccgc
    12061 tatcgtcgca tgggacagtg aattttatga acggttaccg caactgtttc ctcacggcga
    12121 tgcccgcatt tggtttacct ccagcccaca acttgccgaa gaaacagcgt ttcgcaacag
    12181 ttccatgcag gcggcttatc tgatcatcgc ctgccgtgcg ctgggactgg ataccggccc
    12241 gatgtcgggt tttgatcgtc aacacgtaga cgacgctttt tttgcaggca gcacgctgaa
    12301 gagcaatctg ctgattaata tcggctatgg cgatagcagc aaactttttg cgcgcctgcc
    12361 acgtctgtcc tttgaagaag cctgcgggct gttgtaagga gcaaactatg aacattgtcg
    12421 atcaacaaac ttttcgcgat gcgatgtcct gcatgggcgc ggcggtcaat atcatcacca
    12481 cggacggtcc agccgggcga gccgggttca ccgccagcgc cgtctgcagc gtgaccgaca
    12541 cgcctcccac attactggtg tgcctgaatc gtggggcgtc cgtctggccg gtattcaatg
    12601 aaaatcgaac gctgtgtgta aatacactta gcgccgggca ggagccgctt tcaaaccttt
    12661 ttggcggcaa aacgcccatg gaacaccgct ttgccgccgc ccgctggcag actggtgtga
    12721 ccggatgccc gcaactggaa gaggcgctgg tttcgtttga ctgccgtatc agccaggtgg
    12781 tgagcgtcgg cacccacgac attttgtttt gcgccatcga agcgattcat cgtcacgcca
    12841 caccctacgg gctggtgtgg ttcgatcgca gttatcacgc gctgatgcgc cctgcttgtt
    12901 aacctcctta aggagacagc tgatggcaat gttcggtctt cctcactggc agttaaaatc
    12961 gacctctaca gagagcggcg tggtcgcgcc ggatgaacga ttgccgtttg ctcagacggc
    13021 aataatgggc gttcaacacg cggtggcgat gtttggcgcg acggtgttaa tgccgattct
    13081 gatggggctg gatcccaatc tttccatttt aatgtcaggg atcggcacgt tactgttttt
    13141 ctttattacc ggcgggcgcg tgcccagtta tctcggctcc agcgcggctt ttgtcggcgt
    13201 ggtgatcgcg gcaacgggct ttaacggtca gggcattaac ccgaatatca gcattgcgct
    13261 gggcgggatt atcgcctgtg ggctggttta taccgtcatt ggcctggtgg tgatgaaaat
    13321 cggtacgcgc tggattgaac ggctaatgcc gccggtggtg acgggtgcgg tggtgatggc
    13381 gattggcctg aacttagcgc ctatcgcggt aaaaagtgta tcggcttcag gctttgatag
    13441 ctggatggcg gtaatgacgg tgctgtgtat tgggctggtg gcggtgttta cccgtggcat
    13501 gatccagcgg ttattgattc ttgtaggatt aattgtcgcc tgtctgctgt atggcgtgat
    13561 gaccaacgtt ttagggctgg gaaaagccgt cgattttact ctcgtcagcc atgctgcctg
    13621 gttcggtctg ccgcattttt ccactcctgc ttttaatggt caggcaatga tgctgattgc
    13681 acccgtggcg gtgattctgg tggcggaaaa cttaggccat ctcaaggcgg tcgccgggat
    13741 gaccggacgc aatatggatc cgtatatggg acgggcattc gtcggtgatg ggctggcgac
    13801 gatgctttct ggttctgtcg gtggcagcgg cgtaaccacc tacgccgaga atatcggtgt
    13861 aatggccgtg acaaaagtct actcgacgct ggtgtttgtc gctgctgccg tcatcgccat
    13921 gctattgggc ttttcaccga aatttggcgc gctgattcat accattccgg ccgcggtgat
    13981 tggtggtgca tcaattgttg tgttcgggct gattgccgtc gcaggcgcaa ggatatgggt
    14041 acaaaaccgt gtcgatttaa gccagaacgg taatttgatt atggtcgcag taaccttagt
    14101 gctgggcgcg ggtgattttg ctctcacgct gggcggtttt acgttgggag ggatcggtac
    14161 agcaaccttt ggcgcgattt tactcaatgc gttattaagc agaaggttgg ttgacgttcc
    14221 accgccagaa gttgttcatc aggagccata agcagaaaac cgccgggatt accggcggtg
    14281 atgacttttt gacgagctta agaaaaaaat gcattcgtca ttgattagcc cggctagttg
    14341 tcgcttttgc cgtgactgct cttgccacct tttttacctg cttcagatgc gcgctgcgga
    14401 tcattcttga aattaccccc gctgtgctgt ccaccttttt tacctgcttc tgatgctctt
    14461 tcgcggtctt ctgcaaaatt gccggaaccg cctcgatggt ttgccattac tgacctccgt
    14521 ctgggtatag acatcatatt gcatagaatt aacgcggcgt atggcggctg ccatattttc
    14581 gctacgtggg actaagctta gtgaactgac gtgtttctac ccgcgtttgg taatatcctg
    14641 caacagcttc ttagcgagaa tagatatcat ctccaataat tatccataag ccgctcttat
    14701 gattaaaaaa acgcgatgag ataaaaatgt gtcattttgc gacaaaattg cgtgcttgtg
    14761 aaagttgtta taaatcaaat aagtggttgt gaaatttgca ctctgaaaag gacgtcttat
    14821 ctttaaataa gtggtagcga atcgctacgg aatagagata acacgaggag tggttagaaa
    14881 tggctaaagt tctggtgctt tattattcca tgtacggaca tattgaaacg atggcacgcg
    14941 cagtcgctga gggtgcaagc aaagtggatg gcgcagaagt tgtcgttaag cgtgtaccgg
    15001 aaaccatgcc gccgcaatta tttgaaaaag caggcggtaa aacgcaaact gcaccggttg
    15061 caaccccgca agaactggcc gattacgacg ccattatttt tggtacacct acccgctttg
    15121 gcaacatgtc cggtcaaatg cgtaccttcc tcgaccagac gggcggcctg tgggcttccg
    15181 gcgcactata cggaaaactg gcgagcgtct ttagttccac cggtactggc ggcggtcagg
    15241 aacaaaccat cacatccacc tggacgaccc ttgcgcatca cggcatggtg attgtcccca
    15301 ttggctacgc agcgcaggaa ttatttgacg tttcacaggt tcgcggcggt acgccgtacg
    15361 gcgcaaccac catcgcaggc ggtgacggtt cacgccagcc cagccaggaa gaactgtcta
    15421 ttgctcgtta tcaaggggaa tatgtcgcag gtctggcagt taaacttaat ggctaatctt
    15481 caacaggagg atacgcatgc caactcaaga agcgaaagct catcacgtcg gtgaatgggc
    15541 atctctgcgc aatacgtcgc cggaaatagc cgaagccatt ttcgaagtcg ccgggtacga
    15601 cgaaaaaatg gcggaaaaga tttgggaaga aggtagtgat gaagtgttag tcaaagcctt
    15661 tgctaagacc gataaagatt cgcttttttg gggcgaacag accatcgaac gtaaaaacgt
    15721 ttaaccagcg actcccccgc ttctcgcggg ggagttttct gttatttcac cgcttcattc
    15781 aacacgctat caaacttatc catcgggcag aaaccatcag cgtctatcgg gcaaccgctt
    15841 aattccagcg tcacgcgctg cgcaggtgcc tgcagggtta acgcatcggc attacgtaac
    15901 tgttccgcac tctggtacac atattcaatt ttcatcaaat cgcgattggc tttgctgtca
    15961 tgccaacgct ggaaaacgat tttgccgcca atcggcgtgc gttcgttctg gtcatgcaac
    16021 tgatacggtt tgaaatccag cgccgttaac agagaggcaa tgttggagtc atgcccaact
    16081 aacaccgtga tttttggtgc actggtgcga tcggtgacca gggctttgtc gatataactg
    16141 accagcggtt tcgcaacatt gcgcgccact tccggtgagg taaacaggct gtcctggtaa
    16201 ccgtttttca gcttcgacaa caccttccat tgctggtcag atttgatttc tccccaggcc
    16261 acctgatcca tcggaaaacc ttcgtagtat tgcaaagtaa acgcatctac cagcgagttg
    16321 ccgactttca gcggcccgga aacacctggt tcttgttgat acttcgcgct aaaggtattt
    16381 ttgccatcca ccagcgaaca ctgttgtttc tctttacagg caggggaatc tttatagtta
    16441 acgatttttt ccagtagctg gtagctgtcg gtaagctgga gtttgctgag ctctttctcc
    16501 attgccgcca ccgcctgttc actgaatgcg gcggaatcat cggtgatcac cgggttaaag
    16561 gttgggtcca tggtgcccat tttttcctgg tgatgcacag gaatatcaca ccccgggaat
    16621 gcgccggtaa taaagaactg tgcggtcgcg acagtacgtt gcagactatt ggcataggca
    16681 taaacggtgt ccggcggcgg gcattccccc gatttcacca tcccctgctc tgccagccat
    16741 tcacgcatgt aatggcccat atacacttcg agcacgccac ctttggtggt gagttgccca
    16801 ccagggacgt cccattcggg ccatttattc ggcgtcgact gttccagaac actgccattg
    16861 ttcgccagcg gcgcacgtaa gttatggcgg ctcatcatga gcacttgctg tagctgatag
    16921 ccttccggta cggtttgtgc ctgagcattt gaagcgagta aaactatccc tgccacagtt
    16981 gcggcgatta acgttttgtt cattcctggc acctcttttg ttatcaatca ctaaagagtg
    17041 tgacagaaat atgacttttt tccgggaaca ggttcgcaaa atggcctgtt gaacacctgt
    17101 ttttctatat acatcttcaa aaacaggcaa ggtttgacgc aaactatgcc tgaatagccc
    17161 ctgcctcgcg tacgccgctg ataattgcac ctgtggctaa acagaatatc tgcggcgtta
    17221 ccaatacaga gacaatattt aacaataaaa aagtgtgaga ctaataacaa tggtttttag
    17281 catatgcctt tgccattaaa ttgcataacg atttaaatag tcaagttgcg cattcatttc
    17341 cttattgttt aacttgtaag cctgagcaaa aagttcagga gggaggtaaa cgtcataatg
    17401 attgccagtg tttattaaat aaccatactc aagatcttca tctgttgaaa aggattcatt
    17461 cc
//
LOCUS       DAPGRN010000103        17417 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPGRN010000103 DAPGRN010000000
VERSION     DAPGRN010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.104"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(64..795)
                     /gene="rlmB"
                     /locus_tag="RHW33_003584"
     CDS             complement(64..795)
                     /gene="rlmB"
                     /locus_tag="RHW33_003584"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV1647051.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="RHW33_003585"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="RHW33_003585"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV1647052.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDTGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="RHW33_003586"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="RHW33_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV1647053.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="RHW33_003587"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="RHW33_003587"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV1647054.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="RHW33_003588"
     CDS             complement(5488..5685)
                     /locus_tag="RHW33_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV1647055.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="RHW33_003589"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="RHW33_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV1647056.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="RHW33_003590"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="RHW33_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV1647057.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="RHW33_003591"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="RHW33_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV1647058.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="RHW33_003592"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="RHW33_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV1647059.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="RHW33_003593"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="RHW33_003593"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV1647060.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="RHW33_003594"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="RHW33_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV1647061.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEASVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIEFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="RHW33_003595"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="RHW33_003595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV1647062.1"
                     /translation="MMYRIRNWLVATLLLLCAQVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="RHW33_003596"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="RHW33_003596"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV1647063.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="RHW33_003597"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="RHW33_003597"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV1647064.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAPESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDAMLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="RHW33_003598"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="RHW33_003598"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV1647065.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="RHW33_003599"
     CDS             complement(17127..17180)
                     /locus_tag="RHW33_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647066.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3969 a         4977 c         4358 g         4113 t
ORIGIN      
        1 cttaccagct atagacaaaa aaaaaccatc caaatctgga tggcttttca taattcagag
       61 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
      121 cagagaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      181 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaggcgtc cggtcatttt
      241 gctctgatag agtgtatgat ccgcctcacc tgccgtaccg acgatccaga tattctcttc
      301 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      361 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      421 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      481 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      541 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      601 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      661 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      721 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      781 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      841 cggtctacat gatccctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      901 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctgtt
      961 tgaagagggc aatgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcagctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 tgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttacctgta tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcgctg gagatcaggc taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccagacgata
     1321 agtctggccg ctggattcac ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaat aagtcgtcca gacggacaaa
     1441 gaatccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 cataaagtca cacttcagcc agtcagccac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcag catctcttcc atcgaataat ggtagccacc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc aacagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccagaccg aagtgaccac ggttttccgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acgatcggca accgactcca gcagctccgc
     1861 gtagtcgcgc ggttccggct tgttaccgcc cggcagttcc agccccagct ctgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaacttg tgcgcgtcgt tacgctgggt ctgttcgata cgctcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccgccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagcca gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc ggattgagcg agcacagacc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtacc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcagc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaaatcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca gatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtac ggcgagtcgg
     2821 gcgttgggtc agttcaacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcgcc cagcggctga gccagcacct gatcgccatg
     3061 aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg gtcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gtttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaggtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagtcact
     4321 tcacgaccat ccggcatacg gtaagccaca cagagtttca cctctttcag gccatccaga
     4381 acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cagtcgggaa cggacctgca
     4561 cccacacgag tggagtaagc tttgaggata cccagaacgt aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcca ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtcggt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatacggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcag tgacgatatc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag agtcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc acctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttgt cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcagctttat attccggcag aagtttagca aagcgcgcca cttcaccctg ggcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agcagcctgg
     7321 aagttgacgt ccagcagcgt gatacccata tcatacggac gaatcgtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcagata atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acactgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc cttcctgcgg
     8281 tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtagctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gccaacacgg tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctatcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatcgatctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatctc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgcgca tcggactgaa attcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaatcagca acggctgggc
    11221 gcaaacggac gcttcacccg gcgtcaattg tgcctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgaggc
    11521 gggcgcagga gagtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat gggacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gtacgttgac
    11761 gtccacctga tgcgggtcga tctccagata caacacaaac gccggttgtt gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accattcacg tagcaatact gaatttccgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgcagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcgag
    12001 aaaagcggtg ccacaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tttcatcgat atggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgttcccac
    12241 cggatgcgcc gccggtttaa ccgtcacgtc catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtacgatc tggcgtcgtc accgtactgg cagtttgcgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 ataaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgatggg atatccggcg aacgcagaac
    12901 gccaaggctg gcatgttctg gccgacgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg gatcagactg actgttcgcc agcacatcac ccgccccgcc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccaactg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cagaagcgcc agtcgcactt cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgcagccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggtgtttcaa cgcgtttcgc aaccacaggc ggcggaggag gcggtggcgg
    13621 cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccattttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccggag cgaatcgcct tcaccagatt attaccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac ctgcgcgcac agcagcagca acgtcgctac
    13981 caaccaattt ctgatgcgat acatcatcct gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaagt tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatggc ccaaagcctg
    14341 caaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggctgcatca tacggcgata
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcaag gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatagtc agttcatgca ccatcaattc tggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ctcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc aaccccagtg
    15361 agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcatcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 ccggggccaa caaagtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca acaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcgc atctgccgcc tcgcgttctc cccggcggat atcgtcggcg tgccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgccacgg tatgctgcgc gctcgccccc
    16201 atgagttatt gcccggtacg ctgcgcgtga tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttcgttcttc tttttaggcg aattgctggt cgatattcct ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgtggtaaat gcgtggcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aactggaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc acaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggtt cggcgattcg ccgtataggt cacctgcgtt
    16921 ggctgcgtaa tattgctgta gccttaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggaaag tcgtaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt gatcgactcg
    17221 acaaaacgga atcttaattt ttaacttatc tatcaatagg ttaaaaaaat actattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggct gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttgga
//
LOCUS       DAPGRN010000104        17229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPGRN010000104 DAPGRN010000000
VERSION     DAPGRN010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.105"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(247..1248)
                     /locus_tag="RHW33_003600"
     CDS             complement(247..1248)
                     /locus_tag="RHW33_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV1647067.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSTSCCTPAK
                     TSPGTTYTPIKVSCCNPVAKAIEKPVVNCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGTDNPFAIPLSAVIGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRIPMIVAFLTVILGMAILMGYLTQMLF"
     gene            1355..1675
                     /locus_tag="RHW33_003601"
     CDS             1355..1675
                     /locus_tag="RHW33_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1647068.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKTLGEGA
                     TKNK"
     gene            complement(1696..2121)
                     /gene="arsC"
                     /locus_tag="RHW33_003602"
     CDS             complement(1696..2121)
                     /gene="arsC"
                     /locus_tag="RHW33_003602"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV1647069.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTTLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2134..3423)
                     /gene="arsB"
                     /locus_tag="RHW33_003603"
     CDS             complement(2134..3423)
                     /gene="arsB"
                     /locus_tag="RHW33_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV1647070.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSTDFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3478..3831)
                     /gene="arsR"
                     /locus_tag="RHW33_003604"
     CDS             complement(3478..3831)
                     /gene="arsR"
                     /locus_tag="RHW33_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV1647071.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVHN
                     LARQNCSADSKNICS"
     gene            4339..4422
                     /locus_tag="RHW33_003605"
     CDS             4339..4422
                     /locus_tag="RHW33_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV1647072.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4476..5828)
                     /gene="gorA"
                     /locus_tag="RHW33_003606"
     CDS             complement(4476..5828)
                     /gene="gorA"
                     /locus_tag="RHW33_003606"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV1647073.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5900..6742)
                     /gene="rlmJ"
                     /locus_tag="RHW33_003607"
     CDS             complement(5900..6742)
                     /gene="rlmJ"
                     /locus_tag="RHW33_003607"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV1647074.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKSDGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6945..8987
                     /gene="prlC"
                     /locus_tag="RHW33_003608"
     CDS             6945..8987
                     /gene="prlC"
                     /locus_tag="RHW33_003608"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV1647075.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8995..9747
                     /gene="rsmJ"
                     /locus_tag="RHW33_003609"
     CDS             8995..9747
                     /gene="rsmJ"
                     /locus_tag="RHW33_003609"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV1647076.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADVEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9796..11265)
                     /gene="dtpB"
                     /locus_tag="RHW33_003610"
     CDS             complement(9796..11265)
                     /gene="dtpB"
                     /locus_tag="RHW33_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV1647077.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGLSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11582..12016)
                     /gene="uspA"
                     /locus_tag="RHW33_003611"
     CDS             complement(11582..12016)
                     /gene="uspA"
                     /locus_tag="RHW33_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV1647078.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12407..12742
                     /gene="uspB"
                     /locus_tag="RHW33_003612"
     CDS             12407..12742
                     /gene="uspB"
                     /locus_tag="RHW33_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV1647079.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12813..14312)
                     /gene="pitA"
                     /locus_tag="RHW33_003613"
     CDS             complement(12813..14312)
                     /gene="pitA"
                     /locus_tag="RHW33_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV1647080.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14544..15746
                     /locus_tag="RHW33_003614"
     CDS             14544..15746
                     /locus_tag="RHW33_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV1647081.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVEKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVGLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSC"
     gene            complement(16064..17116)
                     /locus_tag="RHW33_003615"
     CDS             complement(16064..17116)
                     /locus_tag="RHW33_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV1647082.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     SSKEHWLWCYWVIFLGSITVLQGIYVLVSSDASTRLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4489 a         4355 c         4443 g         3942 t
ORIGIN      
        1 gactgaaatc tgccctttaa aaggagggat ggtactgaga caaagagaca ttgcttctcc
       61 gataaaatgt cagagaagca tgattacgtt gttattgtct gttgatgact tactgaccgg
      121 gccgtcatca ttctcaaggc attagttaaa tcatttgtga tttttaagga ttatcctatc
      181 tatgattttt tacttagaag gataagacac tcacaatgct ccttatagtt tactgctggc
      241 agctatttaa aatagcattt gagtcaaata gcccattaag atagccatac ctaagataac
      301 cgtcaggaat gcaactatca tcggtattct gaacattgat ttaagtaata tcacttccgt
      361 caggcttgcg cccgcactac cgatgattaa tgccattaat gctcccagac ccattccttt
      421 tgtcatcaaa acagatgcca gagggataac tgcctctgcc cggatataca gcggaatacc
      481 gataacggcg ctcaatggga tggcgaaggg attatctgtc cctgcatgag cggcaatcca
      541 tgcagaggga ataaagccat aaataaaaga gcctattaat acccctaaca aaagatatgg
      601 cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg gattaatact
      661 taccacagct ttggtactgc aacagttaac gacgggtttc tcaatggctt tagccactgg
      721 attacagcaa ctcactttta ttggcgtata tgtcgtcccc ggcgaagttt tggctggagt
      781 acaacaactt gttgatgagc ttttactggc aatgatatga cgttcaaacc ccagagaatc
      841 caggataata ctggcaagta cggagacgcc ggcggcgata atcgcgtaca acaaggtaac
      901 tttccagcca aaggtcaccc acattagtcc gacgataatg ggattaagta acggagaaac
      961 aaataaaaaa gttagagtcg gaccgaaccc agctttcgct gacaacaatc cacgtaacat
     1021 gggtattgtc gagcaactac agaacggggt aacggctccc aacagagcag ccaggagata
     1081 gcctctccct tttctggccc ccatcatctg ctggatttta tggtctggca ccttttgtct
     1141 tatcaggctg acaccggcac taatcactat aaacagcaaa gaaagctcga cggcgagaaa
     1201 cacaaacatc tctgcggcat cttgcagcat cgcaagccaa ctactcatac ataacctcat
     1261 tgaataaatc tatatttcca gaataatcga aatgaaattc agatcaagat atttctggta
     1321 ttatcgaaat atcaaccaga cagaggggtg tcatatgcag ttagaagaag tcgctaaagc
     1381 gctgaaagag ctaggtcacc cgacccgttt gttcatattt aaacatcttg tcaaagcagg
     1441 ggaacaaggg ttgccggtcg gtgagttgca aaagcagctt gggatacctg gctccacact
     1501 cagccaccat atatcagcac tggtatcggt aggactggtc aaacaaaacc gtgagagtcg
     1561 gacgctgatg tgtgtttcgc aatacgcaat gctggaagcg ataattgaat tcttacgcga
     1621 ggagtgctgc gtaaatagca aaacacttgg cgaaggggcc acgaagaaca aataaggggg
     1681 catatccccc ttaagctatt tcaggcgctt acccgcctca tcaaccactt tctcgccatc
     1741 ttccttggag aacgcgcctt tttgcgcatc tggcagaatt tccagcacca cttctgaagg
     1801 gcggcacagg cgagttccca gcgtcgtcac cacaatcggg cgattaatca gaatcgggtg
     1861 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttccgcaa ggcccagctc
     1921 ctcatacggt tcgacgtttt tacgcagcag cgcgcgtacg gaaatcccca tatcggcaat
     1981 aagtttgacc agttcatcgc gcgttggcgg agtttccaga taatggataa tggtcggttc
     2041 tgtgccgctg ttgcggatca tctccagcgt attacgcgac gtgccgcagg ccgggttgtg
     2101 ataaatggta atgttgctca tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc
     2161 cagcgcagcc agcgtcacaa acagcacagg cagggtcatg ataatccctg tacggaagta
     2221 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gcagtgtcgc
     2281 caggctacca atcggggtga ttttcggccc caaatcgcag ccaatcacgt tggcataaac
     2341 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
     2401 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
     2461 cgtcgcggcc cacaggccgt tatccgccag cacgttgagt acgccagaaa gatattccgt
     2521 taatccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
     2581 ctgccagggt gcaccgcgca ggactttacc cgtattaatc gcatgaccgc gtttagcgac
     2641 gacaaacaaa atcagcgcgc ccacagctgc aatggcgctc accggaatgc cgagcggttc
     2701 caggacgaaa aatcccacca acagaagcag taaaacaacc cagcctgttt tgaacgtagc
     2761 aggatcttta atcgcttctg cgggagattt cagcagcgcc atgtcgtagt tctgcggaat
     2821 atctttgcgg aaatagagat gcagcatcac cagcgtggca acaatcgcgg cgatatccac
     2881 cggcaccatc accgaggcat attcgcgaaa gccgaggcca aagaaatcag tggaaacgat
     2941 attcaccagg ttggagacaa taagcggcag gctggcggtg tcggcaatga atccggccgc
     3001 catcacgaac gcaagcgtcg tgcctttact gaatcctaag gccagcagca tggcgatgac
     3061 aatcggcgtc aaaataagcg ccgcgccatc attggcaaac agggcggcaa cggcggcacc
     3121 gagcaggaca atccaggtaa acagcaagcg accgcgtcca ttgccccagc gtgaaacgtg
     3181 cagcgccgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     3241 aataaacgca gccgtcgcgt tccagacgat attccacacc accggaatat cgcccggatg
     3301 tactacgccc gtaactaacg ccagtaccgc gccgagcgtt gcactccagc cgatgcctaa
     3361 acctttcggc tgccagataa ccaatacaat ggtcaggaca aagatagcgc ctgccagtaa
     3421 cataatgcct cccggataaa acacatgtgc aaatccatat gtgtttagct aaatttttta
     3481 actgcaaatg ttcttactgt ctgcggaaca gttttgtcgg gccaggttgt ggacaatcgc
     3541 ctgaaccttt tcctgttcac atcgccaggc ttcatcaata attttcgccg cccatgctgg
     3601 aatatgcggc gataagcggt aatgaaccca cttaccttgc ttgcggtcca gcaatagccc
     3661 gctttcccga agtaacgcca ggtggcggga tatcttgggc tgcgactgat cgagggcagt
     3721 gcagagatcg cagacgcata actctcccag ttcgctgagc agtaaaacga tgcccagacg
     3781 ggtttcatca gcaagaattt tgaacaattg gatgggtaac agaaatgaca tattgcgctc
     3841 ctgattgttg caggtagtgt ctctctttga agcggacaag tcaaaaacat atatgactta
     3901 acgaatatgt aagtgtagag aaaggtatag gtgtgaatta agcgttgggc attcatttga
     3961 aaggaggtct gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt actttccaga
     4021 cctgagttgg tggctctggc tggagtgaga atgccatcat ctaacgcatc aatgctaata
     4081 ccatactgaa actattgcaa ggacatgctg gttttataac ctgaatatac tgtatgatta
     4141 tccagttggc tctgaggcat tttcactctg gcaatgcgca taaacgcttt caaagtcctg
     4201 atcagtggta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa tgccattatc
     4261 taacaatagg gcatgcgccg tgacaggcaa tggatgagta agcggatgca ttctcactcc
     4321 atcgtatgga gaacacgggt gattgataaa gcaatcatcg ttcttggggc gttaattgcg
     4381 ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa acgtaattaa
     4441 gggcaaagag cacaccactc tttgcccttt aacatttaac gcattgtcac gaactcttct
     4501 gccgccgttg ggtgaatggc aaccgtgttg tcgaagtctt ttttggttgc gcccatcttc
     4561 agtgccaccg cgaagccctg caacatttcg tccataccaa agccaatgcc gtgaataccg
     4621 acaatcttct cttccggtcc aacgcatacc agcttcatgc ggcacggctg gcggtgagtg
     4681 gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg atcgtcgccg
     4741 tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg atggctgaag
     4801 accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt aaataggcgt
     4861 tcagagaggc ggcgacccgc tgcaactgct accggtgtca gttccactgc acccgtgtta
     4921 tcgcccaccg cgtaaatacc ttcaatattg gtattttgat atttatcgac gacgatatag
     4981 cctttttcgt tggtttttac gccagcggct tccaggttga tgttgtcatt ggcaggctcg
     5041 cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc cagctccagc
     5101 gtcaggctgc catcggcatt tttcactacc gctttcggga tggcgttggt gtgcaactgc
     5161 ggaccttcgg cgttcatcac ttcgaccagc gtttcagaga tcatcgggtc gaagctgcgc
     5221 agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt aatcacgcca
     5281 gccagctcaa cggcgatgta acccgcgcca acaaccgcca cgcgctctgg caaagcagga
     5341 agggcgaaga agccatcaga atcaataccg tattccacgc ccggaatatc cgggtggctc
     5401 ggacgaccgc cggtggcgat cagaatatga tcggccgtga tggtttcacc gttaacttcc
     5461 agcgttttgg catcaacgaa gcgggcaaaa cctttgatta catcaacgtt atttttaccg
     5521 agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct ggcgatcaac
     5581 gtttcccagt tgaatttatt gatagtggta tcgaaaccat aatccggacc gtacatgtgg
     5641 atcgcttcac ggatttgcgc cgcgtgccac atcactttct tcggtacgca gccgacgttc
     5701 acgcaggtgc cgcccagctc tttggcttca atcagtgcgc atttctggcc gtacatagca
     5761 gcgcggttga tggaggcgat accgccgctg ccgccgccga tggcgatgta atcatagtgt
     5821 ttagtcatga cagagtgtcc ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag
     5881 gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg tgcccggtgc
     5941 ctgccggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgt tgttccagct
     6001 tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga tcgctgtctg
     6061 gcaataccgc cagttcaatt tgcagaattt tgcgaatacc ggtcgcttcc aggtcgtgga
     6121 tcatgcgctt gatttgctga cgcagcacca ccggatacca caatgcgtaa gtgcccgtgg
     6181 caaaacgttt gtaaccttct gctatcccgc tgactaccgc ctgatagtcg gttttcattt
     6241 catacggcgg atcgataagg attaaaccac ggcgagaaac cggcggcagt ttggccttaa
     6301 gctgctggaa accgtcggat ttttcgacgc gcgcacggct gtctttctga aattcagaac
     6361 gtagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtcc tgttcacgca
     6421 gtaactgacg ggcaatcaac ggtgaaccgg ggtagtaacg caactggccg ctacggttaa
     6481 agtgttttac cacattgatg tacgcctcca gctccgcagg caaatcgtcc tgctgccaga
     6541 tacgggcgat accttcgaga tattcgccgg tacgctcggc atgttcgctg cctaactgat
     6601 aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc tctttcagcg
     6661 actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa
     6721 agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat cggcccgtat
     6781 tcggggcgca caaaagcgta tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc
     6841 gtaacgtttc tcattgaaat tcactacact taaccccatg ctacaaacat tatgtaaagc
     6901 gcctgttgag cgcttcctta acctctttaa ccaggactgc gctaatgacg aatccgttac
     6961 tgactccctt tgaattacct ccgttttcta aaattctccc ggaacatgtc gttccagccg
     7021 tgactcaggc gctgaacgac tgccgcgaaa atgtggagcg cgtagtagcg caaggggcac
     7081 cgtacacctg ggaaaatctc tgccagccgc tggcggaagt ggacgatgtg cttgggcgta
     7141 ttttctcccc ggtcagccac ctgaactccg tgaaaaatag cccggaactg cgtgaagcgt
     7201 acgaacaaac cctgccgctg ctgtcggaat acagcacctg ggtagggcaa catgaagggc
     7261 tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta cgccacgctg aacacggcgc
     7321 agaagaaagc ggttgataac gccctgcgtg attttgaact ctctggcata ggtctgccga
     7381 aagagaaaca acagcgctac ggtgagattg cgacccgtct ttccgagttg ggcaaccagt
     7441 acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa actcgtcacc gacgaagcgg
     7501 aactggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggcc gaagcgaaag
     7561 agctggaagg ttatttgctg acgctggata ttccaagcta cctgccggta atgacctact
     7621 gcgacaacca ggctctgcgt gaagagatgt atcgcgctta cagcacccgc gcctccgatc
     7681 aaggcccgaa cgccggtaaa tgggataaca gcaaggtgat ggaagagatc ctcgctctgc
     7741 gtcacgaact ggcgcaactg ctgggctttg aaaactacgc ctttaaatcc cttgccacta
     7801 aaatggcaga aaacccgcag caggtgcttg atttcttaac cgatctggca aaacgcgcgc
     7861 gtccgcaagg tgaaaaagag ctggcgcaac tgcgtgcctt cgctaaagcc gaatttggcg
     7921 tggatgagtt gcagccgtgg gatatcgcgt actacagcga aaaacaaaaa cagcacctct
     7981 acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg gttaacggtc
     8041 tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa gatgttgatg
     8101 tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggca
     8161 gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact
     8221 gcgtaggcca gatgcgtaaa gccgacggtt cgctgcaaaa accggtcgcg tatctgacct
     8281 gtaacttcaa ccgcccggtg aatggtaaac cggcgctgtt tacccatgat gaagtgatca
     8341 ccctgttcca cgagttcggt cacggcctgc accatatgct gacccgcata gaaaccgctg
     8401 gtgtgtctgg tatcagcggg gtgccgtggg atgcggtcga actgccgagc cagtttatgg
     8461 aaaactggtg ctgggagcct gaggcgctgg cgtttatctc cggtcactat gaaaccggcg
     8521 aaccgctgcc gaaggagctg ctggataaaa tgctggcggc gaaaaactac caggcggcgc
     8581 tgtttattct gcgccagctg gagttcggcc tgttcgattt ccgcctccat gccgagttcc
     8641 gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg
     8701 tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg
     8761 gttatgccgc aggttactac agctacctgt gggctgacgt actggcggca gatgctttct
     8821 cgcgctttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca
     8881 ttctgagccg tggcggttca gaagagccga tggatctgtt caaacgcttc cgtggtcgtg
     8941 aaccgcagct ggatgcgatg ctggagcatt acggcattaa gggctgatca ttcagtgaaa
     9001 atctgcttaa ttgatgaaac aggcaccgga gacggtgcct tatctgttct ggcggcccgc
     9061 tgggggctgg agcacgatga agacaacctg atggcgctgg tgttaacgcc ggaacatctg
     9121 gaattgcgca agcgtgatga gccaaaactt ggcggcatct ttgttgattt tgttggcgga
     9181 gcgatggcgc atcgtcgcaa attcggcggt ggtcgtggtg aggcggtggc gaaagcggtg
     9241 ggcattaaag gcgattattt gccggatgtg gtggacgcca ccgcagggct ggggcgcgat
     9301 gcctttgtgc tggcttcagt cggttgccgc gtgcggatgc tggagcgtaa tccggtggtt
     9361 gccgcgctac tcgacgacgg cctggcgcgt ggttatgcgg atgtggaaat cggcggctgg
     9421 ttgcaggagc ggttgcagtt aattcacgcc tccagcctga cggcgctgac cgatattact
     9481 ccgcgtccgc aggtggtcta tctcgacccg atgttcccgc ataagcagaa aagcgcgctg
     9541 gtgaaaaaag agatgcgcgt gtttcagtcg ctggtgggac cggatcttga tgccgatgga
     9601 ttactggagc ctgcacgctt gctggcgacc aaacgcgtgg tggtaaagcg cccggactac
     9661 gcgccaccgc tagcgaatgt cgccacgcca aacgcggtag tcactaaagg gcatcgcttt
     9721 gatatctatg caggtacgcc agtgtaaaag taaagcaggg tagcgataac gctaccctgc
     9781 tttctgcaag aataattaat ggctttccgg cgtcgcaatc atgcgtttca gccacggcac
     9841 catcaacagc atcactactg caacgcccag cgtgaccaga ccaatcttac caaacacgtt
     9901 ggtatagacg ggcaacgtct caagcggatc ggtaatgttg tccggcaccg cggtaaatgt
     9961 tgccacatag ccgcccagca agaacgcggc agcctgcgtc aggaaccaca tcccgagaat
    10021 aaagcccatc aaatgctgcg gcaccagagc agcaatcatc gccaggccaa gggcgctaat
    10081 aaacagttca cctaagctct ggaataagta caccagcacg ataaaccatg gcgatgtcag
    10141 cccttgtgca tccgcaaacc acattcccgc agctgccgcc gtcaaaaagc ccagtgagca
    10201 cataaacatg ccgagagtaa atttcatcgg catcgagagg tctttgcctt tgttacccag
    10261 atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc acccagaacg ggttaagcgc
    10321 ctggaagctg accgggttga tggaaaagcc gagaatttca tgatgcacgt tgttgatggc
    10381 aaagaagttc agcgacgttg gcatctgggc gtagagaatg taaaacacca ccgcttcgag
    10441 catcaggaca aaggcgacaa acattttatt gcgcccggtt ttatccagct tgaatgcctg
    10501 acgaaagaag atgatggtga cgacgatgga gagaacaatc agcaccagat tggcgacttc
    10561 tacgttgtgc atcagccatg cacagacgaa gatcatcacc acgctgccaa gtaacacgta
    10621 caacagtttg ctgaagctca tcggccggaa gtcgggttca gaaccaatgt ctttcaccat
    10681 tccacgacag gcgatgtaaa ccagtaatgc gataattaac cccgcaccgc acaggttgta
    10741 ggtgactgaa tagccgaatt tatcagcgat cacaggggcc agcgataacg ctatcaacga
    10801 gccgatgttg atcgacatat agaacagggt gaatgcgcca tcaagccgcg gatctttcgg
    10861 cggatagcac ttcgaaagca agctggctgg gttagcttta aacaggccgt taccgacagc
    10921 gatagtcccc agggcgatga aaatcaggtc aggcttaagt agcgacaggc cggtcatgaa
    10981 gtagccaatc gccagcacaa gtgctccgag aacaatggtg cgtttggtcc ccagcaggtg
    11041 gtcgccgaca tagccgccaa tggaaatgag gccatagacc agcgcagcaa aagcaccaaa
    11101 agtgacaaaa gcctgctctt gcgagaatcc aagctgttta acgaagaaaa ccgccagtac
    11161 gccctgcacg ccgtagtagc cgaatcgctc ccataactcg acaaaaaaga tcatgaaaaa
    11221 tgggcgaggt tgctgcagca tccccatggg tgttgttgta ttcatattta ttaaccttcc
    11281 aataccatcc tgaaaagtat gaacgcgtaa gggataacgc gcgaaaaagt ttacatctgt
    11341 cagagattct taatattgat gcggtgaata gattaataca ctattacaac agaaaataac
    11401 cagctgatca cactaatgat ggaaaatatg atggtgttaa atattgtatt ggctggggat
    11461 tttgattgcg aacgcagacc gtaggccaga taaggcgttt acgctgatca ggtaacaagg
    11521 cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggagggaag
    11581 attattcttc ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca
    11641 gctgacgtgc ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacca
    11701 gatccatatc gtatttcttg attgcatcga ccagaacctg gcccaggtcg ccgctgccgc
    11761 tcagggtttc agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg
    11821 tctcttcaga gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata
    11881 ggtcagagta gtttacatct acgtggatca gagaaacttt cgcattgtat gggcgagcca
    11941 tagagactgc tttctctacc agaactttgc tttccgggga gagatcgacc gcgatgagaa
    12001 tgtgtttata agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc
    12061 gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc
    12121 cgtaaagatt cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc
    12181 cgccttgctt acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac
    12241 cttctggaaa aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt
    12301 caccacaaaa gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg
    12361 gtagccccgg ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt
    12421 cgcattattt tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc
    12481 actacgcgcg ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga
    12541 tggagggggc ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta
    12601 tatctatgcc caacgttatc gcgatcatca cgatgatgag tttattcgcc gctgtgagcg
    12661 ggtgcgtcgg cagtttattc tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat
    12721 tgccttgatg atttggcatt aagcacttca gcaataataa cgcataaaaa aagcgggcca
    12781 gtaaactgac ccgctcattt tggtgcgtac gattacagga actgcaagga gagccagtac
    12841 agcccgccgg aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatactg
    12901 gttacggttt tacgctgtaa gccgccacca tctaccacca tcgtccccgc gacagaagag
    12961 gagagtacgt gagtagtgga aaccggcatc ccggtataac tcgccaggcc gatggacact
    13021 gccgccgtca tctgggcaga catcccctga gcgtaggtca tgcctttctt accgattttc
    13081 tcaccgatag tcgttgccac acgacgccag ccaatcatcg taccgatacc taacgccagc
    13141 gcgaccgcca tgatgatcca caccggtgca tactcgatgg tgctaagcat gtcggacttc
    13201 agtttcttca acaggcgctg atcgtcagca ctcacaccag gcatcttcac cactttgtcg
    13261 atagtgtcag aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc
    13321 ttgtcgtagc tttccacatc ggtagtcaac atgcctttca ggcggttgag cgcgttaatg
    13381 gtattcgacg gatggcaatg gaactccgca ggttgcgttg cgccagcttc cggagccggt
    13441 actaactgat cagcaccggt agcttgtttc agcagcgcag gatgctgctc aaagtaagct
    13501 tcgacgttgt tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc
    13561 accacgaagc ctgctggtgc gacaccaatc aataccaaca taaccagacc aatgcctttc
    13621 tgaccatcgt tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcgata
    13681 cgcgtccaga acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg
    13741 tggatacggg cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg
    13801 ccagcaaaca ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact
    13861 ttcgggatat tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca
    13921 atcccgatga tcgcgccaat cagcgtatga gagctggatg caggcaaacc aaagtaccag
    13981 gtacccaggt tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga
    14041 gacgatccca tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca
    14101 ctcagaccac ccagcaaaac acccaaaaag ttgaacaccg ctgccataac cacggcgagc
    14161 tgagaacgca tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa
    14221 ccattgatgg cttcgtagaa cagtacaaaa gccagtgcaa gcaataataa cagcccggta
    14281 tgcaaatcca ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga
    14341 acgcggcgca ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg
    14401 atttgcctcc tgtatggatt tcactcaaaa aataattatc ttatataatt caggcaaata
    14461 cttcctttta gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc
    14521 gttttttcta agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg
    14581 cggcgggtat gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg
    14641 ataatggtaa aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta
    14701 ccaaccttta tgtcgaaccg ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg
    14761 cactcgcgcg ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct
    14821 ggcacgagaa aacgttaggg caactcttct gcgatgactc cgctcagcag attgtcgaca
    14881 tgctggtgga tgagtgcgag aagggcaatg tgaccttcag attgcgtagc gaagtgctga
    14941 gcgtggagaa ggatgataca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg
    15001 aaaagctggt catcgcgacc ggtgggctgt caatgccggg gctgggcgca tcgccgtttg
    15061 gttataagat tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc
    15121 cattcactct gcataaaccg ttgctcgaag agttacaggt gctggcaggc gtggcggtgc
    15181 cttccgtgat taccgctgaa aacggcaccg ttttccgtga gaacttactc ttcacccatc
    15241 gcggcttatc tggaccggcg gtgttgcaga tttccagcta ctggcaaccg ggtgaatttg
    15301 tcagtatcaa tctgctaccg gatgtgggcc tcgaaacctt cctgaatgag cagcgtaacg
    15361 cacatccgaa tcaaagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg
    15421 aacgcttaca gcaactcggg caaatcccgg atgtctcgct aaaacagctc aacgtgcgtg
    15481 accaacaggc actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag
    15541 gctatcgcac tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga
    15601 cgatggaagc gcgcaaagtg cctgggctgt actttatcgg cgaagtgatg gacgtcaccg
    15661 gctggctggg gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg
    15721 atttgattgc agcgaagtcg tgctgagcta tttttcctaa agatgaaaat tattagagtc
    15781 gcattaaaaa tgagctaatt ttgatagtgg ctatcttgtg attattttct aatgagcccg
    15841 tgaactgaac cccccatgct taatataagg tggagggaaa ggtgattgaa aactcaccca
    15901 gtgggctgat ctttataaaa tatgaacagg ttgaaaaaaa aacagtaact ttgctgtttt
    15961 ttatacttaa ccactatgca ttaatgctgc gttatttcat tctacctaag aaaaaccagc
    16021 gttacgcaaa tggtcaacgc tggttttatc cggtacgttg caattatttt ttagcagaac
    16081 ccgcttctaa tattgaaacg attgagaaca acgtaaagca taccgctccc aaaccgacca
    16141 gaacccgcga aggaataaaa tatcccatat cggtctgcgc catttccgca agaaacgatg
    16201 ccaggaaaag gcaaaacagg caggtgaaga cgggaatcat cggtattctg ttggctaacg
    16261 aacaggtacg tctccagacc agtgccagta gccagacttt tgagaatatg ctgtaacaga
    16321 tcattccgag gcaaatgaga ataatgccgg gggccagtcg ggtgcttgca tcggaactga
    16381 ctaacacgta tatgccctgc agcaccgtga ttgagccgag aaaaataacc caataacacc
    16441 acagccagtg ctctttggaa gagaacgtat tacgtgtttg atggacgatt gtggcaacaa
    16501 ggccaattag acaggcacag attgcggtta acccgagcaa tacgtgaccg gcgacataat
    16561 gcggagttat gtcggcacta cgtaacagcg taatagacca aatgaaaccg agcagggtga
    16621 gtaacacggg aacggcaatg aggcaattac cgattaatga agaataagcc tgtaccggtg
    16681 ttccgtcgct cttgctcccc gctgcgtttt taggaatgag cagaaagtga ccggatgacg
    16741 ctgccaccgt cgatacacag gcggcaatca taccaacgcc gaaaataaca tggccggcga
    16801 caaactcgtc tgccatcaca tcattacctg ctaacagtgc ccaaccccat atcatggtga
    16861 taattgaccc cgcatagcca ataatgggga acaatgtatt gtaaaacgta ttaacgccgc
    16921 gcgtgagctg cgaaatgata ataaatgcgg tagtgaataa tgccaggcat atggctgcca
    16981 gagaaatgag cacatgaccc gcgacgaaat actcgctttg ccctgaactt tccagcacaa
    17041 agccacctaa cgcgatacaa atcaagccca taattaaagg gattaatttg aaaagccacc
    17101 cgatatatat gttcacttta aactcctcag gtatccctgc taatattgaa atgtgcatta
    17161 atgataattt cagcctgtta atacaatgac gagatatcat taaagacaaa tgcatagcat
    17221 aactatagc
//
LOCUS       DAPGRN010000105        16574 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPGRN010000105 DAPGRN010000000
VERSION     DAPGRN010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.106"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(76..228)
                     /locus_tag="RHW33_003616"
     CDS             complement(76..228)
                     /locus_tag="RHW33_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1647083.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(494..1228)
                     /gene="cysH"
                     /locus_tag="RHW33_003617"
     CDS             complement(494..1228)
                     /gene="cysH"
                     /locus_tag="RHW33_003617"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV1647084.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALRDLNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1302..3014)
                     /gene="cysI"
                     /locus_tag="RHW33_003618"
     CDS             complement(1302..3014)
                     /gene="cysI"
                     /locus_tag="RHW33_003618"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV1647085.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIERIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3014..4813)
                     /gene="cysJ"
                     /locus_tag="RHW33_003619"
     CDS             complement(3014..4813)
                     /gene="cysJ"
                     /locus_tag="RHW33_003619"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV1647086.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQITSEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5132..5494
                     /gene="queD"
                     /locus_tag="RHW33_003620"
     CDS             5132..5494
                     /gene="queD"
                     /locus_tag="RHW33_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV1647087.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5572..6843
                     /locus_tag="RHW33_003621"
     CDS             5572..6843
                     /locus_tag="RHW33_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV1647088.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVVEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTYPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLLWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6834..7094
                     /locus_tag="RHW33_003622"
     CDS             6834..7094
                     /locus_tag="RHW33_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV1647089.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAERLINACPAGLFALTPE
                     GDLRVDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7111..7686
                     /locus_tag="RHW33_003623"
     CDS             7111..7686
                     /locus_tag="RHW33_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV1647090.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRQFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7833..8693)
                     /locus_tag="RHW33_003624"
     CDS             complement(7833..8693)
                     /locus_tag="RHW33_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV1647091.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTISVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8690..9469)
                     /locus_tag="RHW33_003625"
     CDS             complement(8690..9469)
                     /locus_tag="RHW33_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV1647092.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAAELRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCRQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9447..10856)
                     /locus_tag="RHW33_003626"
     CDS             complement(9447..10856)
                     /locus_tag="RHW33_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647093.1"
                     /translation="MMGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGVLLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAAMPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLILAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10878..12332)
                     /locus_tag="RHW33_003627"
     CDS             complement(10878..12332)
                     /locus_tag="RHW33_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV1647094.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12402..13187)
                     /locus_tag="RHW33_003628"
     CDS             complement(12402..13187)
                     /locus_tag="RHW33_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1647095.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKKMIENQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASQASNYVNGHLLVVDGGYLVR"
     gene            13505..14782
                     /locus_tag="RHW33_003629"
     CDS             13505..14782
                     /locus_tag="RHW33_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647096.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADIAPTTANSK"
     gene            14809..16287
                     /locus_tag="RHW33_003630"
     CDS             14809..16287
                     /locus_tag="RHW33_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV1647097.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPETLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DTMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3938 a         4363 c         4169 g         4104 t
ORIGIN      
        1 atataaattt aaaaaagggt gcaggagacc aaaccatcac aattcaatac gcggggtttt
       61 acccccgcgt ggtggctact tcttcgattc gtaagcgaga actgccttaa actccatacc
      121 acgctctctg atactcaact cacataacga gtcgcctacc atgagcgtga atcccagtac
      181 tgtacaacac agtactatga ttgccacaag ggcatatttt gttagcatgt cttgcctccc
      241 tgcaaagaag agactaacat ccgaattgct taggttcgaa cgctggcctc aggttgatag
      301 aaatatcgcc tggggctttt gtccatctgg aacctcgcga atgcttaacg ccagacagcc
      361 tcaagcaccc gacgccattc tatgcctgta gattcccctt gctacattca catttaaaag
      421 caggaatgct gctcgcaaaa taaatacgcc cccatagttt ccgacagagg cgcttattaa
      481 cggtaccgat ttattagcct tcgtgtaatc cacattccct ttttaagcca aagaaacgcg
      541 tttcttcttc agccatgccg ggttcccatt tacgggttgt atgggtatca ccaaccgata
      601 aatatccttc atcccataat gggtgatatt taaggccatg tttttgcagg tactgataaa
      661 tagttcggtt atcccagtcg ataatcggca gcactttaaa tacgccacgc tgaattgcca
      721 gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg caggccagca aaccacgttt
      781 gcgcattcag atctctcagc gcccggttca tcggttcgac tttgttgatg tcattgtact
      841 tttcaatgcc ttcaacgccc tgctcccaca gtttgccgta gcgtgcttcc tgccaggcgg
      901 cgctttcggt agcacggtac actttcaggt tgagcttgag tttgtccgtt aactcatcga
      961 taaagcggta ggtttctggg aacagataac cggtatcggt gaggatcact ggaatatccg
     1021 ggcgaatctg attcaccaga tgcaggctca ccgccgcctg aatgccaaag ctggaagaaa
     1081 gtacatattc accgggcaga ttatccagcg cccaggcaac gcggccttca gcatccagtt
     1141 tttccagttg ggcgttagtt tctgccagcg ccagaatgcg atccaccttc ggcagttcgt
     1201 tcagggcgtt tagatcgagt ttggacatag attcctcact gtttgccttg cctgatgcga
     1261 cgctcacacg tcttatcagg cctacaagac cgggctgatg gttaatccca caaatcgcgc
     1321 gccggatcga gcaccgggcg aatgatgccc gcacgcaccg taaagtcgcc gaagccttca
     1381 cccgcttcgc gctctttcgc ccagcgccct atcagttcat caagcgacgc caggatttcc
     1441 ggctcggtga tgttttcttt atacatccgt gggatacgtg tcccaatacg gttgccgcca
     1501 agatgcaggt tgtagcgacc cggcgcttta cccaccaggc ccacttccgc cagcatcgcg
     1561 cgaccacaac cgttcgggca gcctgttaca cgcatcacga tatgctcatc gctgacacca
     1621 tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg gcaggaaacg ctccgcttcc
     1681 gccatcgcca gcgggcaagt cgggaatgac acgcaggcca ttgagttttc acgctgcggc
     1741 gtgacggcat tcattaaccc gctctctttg gcgatcctct cgatcttcgc tttctcactt
     1801 tccggcacac cggcgatgat cagattctgg ttcgccgtaa tgcggaaatc acctttgtgg
     1861 atctttgcga tctccagcag gccggttttc agcggacgcc caggataatc aaggatgcga
     1921 ccattttcga taaacagcgt caggtgccag ttatcatcaa ttcccttaac ccagccaata
     1981 cgatctccgc gtccggtgaa ctcatacgga cggatcggtt caaatttgat ccccgcgcga
     2041 cgctccactt ccgctttaaa cgtctcaacc cccacgcgct ccagcgtgta tttggttttg
     2101 gcatttttac gatcggttcg gttaccccag tcacgctgag ttgtcacgac agcttccgcc
     2161 accgccagcg tatgctccag cggcagatag ccaaactcgc ttgcggtgcg ggcgtaggtt
     2221 ttcttgttgc cgtgttcgat agaaagccca ccgcccacca acaggttaaa gcccaccagc
     2281 ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg cgtgcagatc gatatcgttc
     2341 tgtggcggga tcactaccgt ggttttgaat ttacgcggca ggtaggtctg gccgaggatc
     2401 ggttcttcat cagtggtggc gactttttcc tgatcgagcc agatctccgc atacgcgcgg
     2461 gtacgcggca gcagatgttc cgagattttc ttcgcccact cgtacgcttc tgcgtgcagc
     2521 tgcgactcgt aagggttcga ggtgcagagt acgttacggt tcatgtcgtt agcggtcgcc
     2581 agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg gtttgacgtt ctttttcaga
     2641 atgccgtgga actggaatgt ctggcggttg gtcaggcgaa tgctgccata gatggtgttt
     2701 tcaccggcaa atttatcgat cgcctgccac tgtttggtgg tgataactcc acccggcaga
     2761 cggcagcgca acagcatcgc atggcgcggc tccagcttct gttcagcacg ttcggcgcgg
     2821 atgtcgcggt catcctgctg atacatgccg tggaagcgga tcagcaggaa gttgtcgccc
     2881 ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa tggtgccgcg caggtagttg
     2941 ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt cgaccactaa aggccctgga
     3001 tgtttttcgc tcattagtag acatctcgct gataacggcg ctctacgcgc agctcactta
     3061 aaaattcatc cgccgcttcg gtgtccatac caccaaattc ggcaatcact tccagaagtg
     3121 cctgctcaac gtctttcgcc atgcgattag cgtcgccgca gacataaatg tgggcaccgt
     3181 cattgatcca gcgccacagc tccgcgccct gttcgcgtag tttgtcttgt acgtaaactt
     3241 tttctttttg atcgcgcgac caggcgagat cgatacgtgt cagcacaccc tctttgacgt
     3301 agcgctgcca ctccacctgg tagaggaaat cttccgtaaa atgcgggttg ccaaagaaca
     3361 gccagttttt acccggtgct tcatcggcag cacgttgctg cataaaggcg cggaacggcg
     3421 cgataccggt gcctgggcca atcataatca ccggggtttc tggattggct ggtaggcgga
     3481 agttatcgtt atgttcgata aatacgcgga gttcgccctc ttcttccacg cggtccgcaa
     3541 ggaagctgga ggcccccccg gcacgggcgc ggccttccac gtcgtaacgc accacaccaa
     3601 cggtgacgtg tacttcgttc tcgacttccg cctgcgagga ggcaatggaa tagagacgcg
     3661 gcgtcagcgg gcgcagcaga ttaattagcg cttcggcatc aagctgtgcc ggggagaaac
     3721 gcaccatgtc aacaatcggc gtcgtcgcgg cgtaatgctg taacttcgct ttatcgccca
     3781 ccagcggcag cagtgtttca ctgcgggtaa gcgtggcgta attctcaaca atgttggcgg
     3841 tgttgacggt cagttcgaag tgccactgta gcgcttcgtt cagaggcaac gttttgccct
     3901 cgacggtgac aggttcatcg cctttcagcc acagcagttc gacaagttct ttcaccagtg
     3961 ccggatcgtt ctgataccag acacccagcg cgtcacccgg ctggtaacgc aggcccgagt
     4021 cacctaagtc aatttcgata tggcgaacgt ctttttcaga gttacgcccg gtaattttct
     4081 ggttaaccga aaggctcgct accagcggcg cgtctttgct gtacgggctg gtgtggattt
     4141 catttaccgc gccagtagcg acggattgcg aaggtgccgc gacaggcgca cgcgatttaa
     4201 gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc ctggtattca acgtcggcgt
     4261 cgacacggtc gagcaggcgt tcaccgccca gttccgccag cttgctgtcg aaatctttcc
     4321 cggactggca gaaaaattca taagaactat cgccgaggct aaacacggca aaagcggtat
     4381 tttccagctt cggcgctttt ttggagaaca ggaacttatg cagcgcgacg gcttcttccg
     4441 gcggttcccc ttctccttgc gttgacgtca ccacgataag cagtttttcg ctggtgattt
     4501 gtttgaattt atagtcgccc gcgttcacca gcttaacgtt cagttttgcc gtcaataaat
     4561 catcgcgtaa tgcttcagca actcggcgcg cattgccggt ttgcgaagca gagataatag
     4621 ttatacccgg catttctgcg gctggcgctg gcgtcgctgc aagcgcagca ggctgctgat
     4681 tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg ggtgggagtt aaatcggtcg
     4741 tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg aagcaacgcg gaaggtggga
     4801 cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc tgtttctgcg ccctgtcagc
     4861 gcccataaaa cagaagagaa ggtaaggtta acggggtgaa cggtgtggat taaagacggg
     4921 atagcgataa ctaataacca aatcgactaa cctgttttag caatagtctt taacaacaaa
     4981 atagattaac caacctaatg aaaaacaaat gaatttagcc aatcattaag ataaatcagc
     5041 gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa cagcctttcc ggtactctac
     5101 ggcggtttta tcgtcctgta gagaaattat gatgtccacc acgttattta aagatttcac
     5161 cttcgaagcc gctcaccgct taccacacgt cccggaaggg cataaatgtg gtcgcctgca
     5221 cgggcattcc tttatggtgc gactggaaat taccggggaa gtcgatccgc atacgggctg
     5281 gattatcgat ttcgctgaac tgaaagcagc gtttaagcca acttatgagc gcctcgatca
     5341 ccattatctc aatgatattc caggcctgga aaacccgacc agcgaagttt tagcaaaatg
     5401 gatttgggat caggttaaac ccgttgtgcc gctgttaagt gcggtgatgg taaaagaaac
     5461 ctgcaccgct ggttgtatct atcgcggcga atgataagag tgtgtcggcg gtcaatttcc
     5521 cttaagtaac gctatgttag ggtgttgtgt tctggatatc tggggcatga catggaagac
     5581 gactgcgaca ttattattat tggtgccggt attgcaggca ccgcttgcgc gttacgctgc
     5641 gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg aaatccccgg cagcaaaaat
     5701 ctttccggcg ggcggttata tacccatgca ctcgcggaac tcctccctca gtttcatctg
     5761 accgcgcctc ttgaacgacg catcactcac gaaagccttt ccctgttaac gccggatggc
     5821 gcaacgacgt tttccagctt acagcccggc ggtgaatcct ggagtgtatt acgtgcgcgg
     5881 ttcgatccgt ggctggttgt cgaagccgaa aaagaaggtg tcgaatgcat ccctggtgcg
     5941 acggtggatg cactgtatga agaaaacggc agggtgtgtg gtgtcatttg tggtgacgat
     6001 attctccgcg cccgttatgt ggtgctggca gaaggtgcca acagcgtcct ggctgaacgt
     6061 cacggattag tgactcgtcc tgctggcgaa gcgatggcgt tggggatcaa agaagtgctg
     6121 tcgctggaaa catccgctat tgaagaacgt tttcatctgg agaataacga aggcgcagcg
     6181 ttgctgttca gcggcgggat ctgtgatgac ttacccggcg gcgcatttct ttatactaat
     6241 caacaaacgc tctcgttagg gattgtttgc ccgctctctt cccttacgca aagtcgtgtt
     6301 ccggcaagcg agctgctggc tcgctttaaa acgtatccgg cagtgcgccc gcttatcaaa
     6361 aacacggaat cactggagta tggtgcgcat ctggtgccag aaggtggctt gcacagtatg
     6421 ccggtgcaat acgccggtaa cggctggctg ctggtgggcg atgcgttgcg cagttgcgtc
     6481 aataccggaa tttccgtgcg cggcatggat atggcgctga ctggcgcgca ggcggcggca
     6541 caaacgctga taagcgcctg ccagcaccgc gagccgcaaa atctgtttgc gctttatcat
     6601 cacaacgtcg agcgcagcct gctgtgggat gttctacaac gttatcagca tgttccggcg
     6661 cttttgcaac gcccgggatg gtaccgtgcg tggcctgcgt taatgcagga tatttcccgc
     6721 gatttatggg atcagggtga taaacctgtt ccaccgctgc gccagttact ctggcgtcat
     6781 ttacgtcgtc atggcttgtg gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg
     6841 tagcccgtaa tctctggcgc gctgctgatg cgccgcatat tgttccggct gactccgttg
     6901 agcgccagac ggcagaacga ttaattaacg cctgcccggc aggtcttttt gcgctcacgc
     6961 cggaaggcga cttgcgtgtt gattatcgcg gttgccttga gtgcggcacc tgccgtctgc
     7021 tgtgcgacga atccacacta caacagtggc gctatccgcc ttccggattc ggcatcacct
     7081 accgctttgg ataaataata aggataattt atgcccctct tacacctgct ccgccagaat
     7141 ccggtgattg ctgccgttaa agataatgcc agcctgcaac tggcaatcga ttctgaatgc
     7201 cagtttattt ccgtgttgta cggcaatatc tgcaccatca gtaatatcgt taaaaagatt
     7261 aagaacgccg ggaaatatgc ttttattcat gttgatttgc tggaaggcgc gtcaaataaa
     7321 gaagtcgtta ttcagttttt gaaactggtg accgaggcgg acggcattat cagtaccaaa
     7381 gcctcaatgt tgaaagcggc aagagcggaa ggttttttct gtattcatcg ccagtttatt
     7441 gttgattcaa tttcgtttca caacattgat aagcaagttg cgcaatcgaa tccggattgt
     7501 attgagatcc tgccaggctg tatgcccaaa gtgctgggct gggtgacgga gaaaatccgc
     7561 caaccgctga ttgccggtgg gctggtgtgc gatgaagaag atgcgcgtaa tgcgattaac
     7621 gcgggtgtcg tggcgctttc caccacgaat accggagtct ggacgttagc gaaaaaattg
     7681 ctttgacggg atattcgtaa cgaattgaat ttgtgtgatt tgtaggctgc acgccacatc
     7741 tgacattcag cgcctgatgc gacgcttgac gcgtcttatc aggcctacag gtccccaggc
     7801 cagaatcgta ggcaggatca tccggcaaca cgttattggc aatctgcgtg aatgtttgtc
     7861 accagtgcct ccagcaccac tttccagtca tcaaccacgc caacatctgc ctgtgaaaat
     7921 accgctgcgc tggcgtcatg attaatcgcc accacaaact tgctattgcg tacgcccgcc
     7981 attaacgctg ccgcaccgga tgcgccaaca acaatacaca cctcaggtgc cagcagatgt
     8041 ccggaaatac caatcacttt ttcggcatca acgccaccgt tcatcactcg tgcccgactg
     8101 tagcctactt cggctcccag cttttctgcc agcatagcaa tttcctggtt atctgcctct
     8161 ccgccctgcc ccaccaccag cacccgtctg gcctcagcta atgggtcgcg agtaacattt
     8221 ttcagatcct ctgtgctaac cagccagtcc ggaagcgcac ccgggacaat gttcagttgt
     8281 tgcattccag agggcaacgt ggcgtttttc gctgctcccg cctgacgcgc cagggaaagg
     8341 catagcgggc gtttttctgt ttgcagcgtc gccgttagcg catttcccca gtgggatttg
     8401 cgcacgctta tggttggaat atctaacgaa gtcacctggc agatgcttgc accatgtaaa
     8461 cgccaggcca gccgggtcga cagttcatcg ccaaacgttc ccggcggaaa gagaacaacg
     8521 tccgcaggcg ttcgctgcca ctgttccacc aacgcatcca gcacctgttc cgccactaca
     8581 ggctgaggtt caatctgcca gtgcgccagg gtacaaccgc tgaaatcctg tgccgccagc
     8641 cagcttgcta ttgcggcatt ctcctgattt attgtcacaa ttgcgatatt catggctgca
     8701 tcctttggcg taaatagtcc tgccatagct tttgcgcttt ttctgctacc gtttgcccat
     8761 caatcagcgt cgctccacgt cgttgctctg ctcttgccag ttgccgacac tgcatcgctg
     8821 gcatttcggc agcaacagtt ttacggataa tttctgcttt ccccgcagcc attcgctggc
     8881 gcattcccgg tactggcaag gccacttcac cgcactgacg cacggcaatc accgcaggca
     8941 agcgaacccg gcaacaacgc aacccatgtt cagtacgctg ttcgagggtg ataaacagcg
     9001 cgtcgagagt gaaacgctcc acctgggtga agcagggcca gcccagcatt tccgccagca
     9061 aaaatggcgt ttgcccattc tgcccttcgc tgctttggca gccggtaatt atcagatcca
     9121 gcggattctg acgctgccat tcggcaatat ggcgagcgac aaattccggt gcaaagcgca
     9181 gttctgccgc cgtctccagc aatacagctt cctcaaaccc aagagccatg agatagcgca
     9241 gccagtgcaa cgcccgttca tcccccatgc tcaacgcggt taaagacatc ggcgtaccgt
     9301 tttttctctg cgccaacagc agcgcggcgg cagcctgttc atcagcaccg agtaaacttc
     9361 gcagtagcga aacatccggt ccgctattac cctgagccgc cgcctgccac tctttttccg
     9421 ccagcattcc ggcatccggt tcggctttaa acgctaacag aatgttcatt cgcacctcct
     9481 acatttcccg ccgccaccag cgggagtgct ttggtttctg gtgcccatag ccaggtcacg
     9541 acaaaaccaa ctaacaacac tgccgccaga atcaataagg taacctgcat tccccactgc
     9601 gccagcaccc acggcagcag gccagtactt accgccgcgc caagtcgact catggcggtg
     9661 gcaaaaccga cgcccagcga gcgaatgtca gtaggaaaac tttccgcagg caaaatgccc
     9721 accagattac tgactgccga aatggtggtg ctgaagagaa caaaaagcag cagcgttaat
     9781 gaactgccgg aaggcaaaca ggccattact accagcgttg ccgccagcag caaaaaactt
     9841 cccagcaaaa atttgcgatg tgccagcagg tgcgtcagaa ctaatcccag cagcgcgccc
     9901 acaattaaca acgcattaag catcaggctg gcagtcagcg catcttccag accaatagtc
     9961 tgggcgatag ttggcagcca ggtataaatc acaaaccatg ggattacgag gcagacaaag
    10021 aagacgctgt taaacgccgt gcgccgccag tagcgcgaag agaacaaggt tttgatgtgt
    10081 ttatgggtcg ccgttgccac ttcatcgccc agtaaaacat ggggaccaaa atagcgatgc
    10141 acgatagcgt gagcttctgc aaaacgcccc tggcgcagta gccagcgtgg cgattctggc
    10201 gttccccagc gtaataacgt aatcaacaac gcgggcatag ctgccgatgc cagcagccag
    10261 cgccaggcct ccgggctttc gctaataaag tgatgtccgg caatacttgc taacacatag
    10321 cctacggtcc acaccacgct gaatgcgccc agcaaaacac cgcgatggcg gcgcggggaa
    10381 aattcagcca gcaaggtgtg acctactgag taatcgcctc ccagaccaat gccgatcaaa
    10441 atgcgcagtc caataagatg ctctggcgtg gtggcaaaaa attgcaagaa cgaagcgagc
    10501 gtaatcagca aaaagctgaa ggtgaagatt ttttgccgac caatatggtc ggagatccac
    10561 ccaagaacca ggctaccaag aaacaaacca aggagcgccg agccgccgat cattcccgcc
    10621 ataaacggcg tcagttgcat ggcgggcgta agctgaataa tggcgtaacc aatgacgccg
    10681 agaacataac cgtcggtcag gtgtgcgccg aaagtgagcg cagcaattcg acagtgaaaa
    10741 cggttaagcg gtaaatcatc cattcgcacc ggtgaagtgt tcatctttac cccttcattg
    10801 tccggtaata agaacggctt ctcgcctgag aagccgaaaa ggcaacgccc catcatcgcg
    10861 gaacgctgcc tgacacatta tttttcaatc ggatagatag tacccgtatt cataatgccg
    10921 ttaggatcga attgcttttt cagcccttcc agcaacgccc acgcgctgcc gtgttccagc
    10981 ttgctccagt gaacgcgatg tttaccaatg ccgtggtggt gcaccatcga accgccgagg
    11041 cgaatggttt cttcgcagat gattttgttg agcggattgt ggtacttgtc gatttcctct
    11101 tccggcttac agtcaacaac gttgtagtca tagacgaagt acatgtttgt gccgttctga
    11161 tagctatgag aggagtgacc gcccagcatg gtgatgtcgt cggcgtgcgg gaactcggta
    11221 cgaatacggt taataacact ttcgtagatt tcgtgaatgc agctccagca gccggatact
    11281 tcggtggtaa agcccatgtt gccggttttg aggatctgca cacgttcggc agccacttta
    11341 tccggtcccc agttcaggtt gttaaaccag gtttcgatca gcttgctgtc cacgcgctgg
    11401 cattgcgggt agcgggcaac gatttccgca atcccttcgc ccgtcgcctt cgcaatgcga
    11461 gggttacctt cagccataaa gatcagcacg cattttccgt cggcaaaatg ggtgaagtgt
    11521 tgggtgccat cttcagcgtc atacaaacga gcgatcgacg gacgataccc ttccaccatg
    11581 atttcacgca ggatgttgaa gccggttttc atgtcttcca ggatatagcc gtagaagagg
    11641 ttgttttccg gggtgaattt aaagattttc actgttactt cagtgatata gcacaatgca
    11701 ccttcgttgc cgatgatgat gtgacgaatg tccgggccag ccgcgcggcg tggcacgttt
    11761 ttaatacgtg tgacggtgcc atcagccaat acggcttcca gaccaacgac catatcttcg
    11821 attgcgccgt agagtgtgga gaactgcccg atactgcggg ttgctaccag gccgcccatc
    11881 tgcgccagcg gctttgactg cggagaatgc cccgtggtgt aacctttttc acgcaacgcg
    11941 ttttccagca cctccagcgg aacgccacat tgcgccgtcg cctgcatatt ctcaatatca
    12001 atattaatga tttgattcat ggcggaaccg tcgagcacca ccgagttttc tacaacagtt
    12061 tccagcccac cttcggtggc ggaagcaccg gtacgcggca caccgttaat tttgtgcgca
    12121 ttcataaaat tcagcacacg ggagacttgt tctgtggaac cgagttttac gaccgcagcc
    12181 ggaatcggca aagtataaat accatgaata tccggaaatt tacgaaaacg gtcaatactg
    12241 ttcttcttta atactgtttc atcggtaatt acgcgatctg caccaacaat ttcctttagc
    12301 tggtcgacaa tcgctgcgcg agataaagac atagtaattc cttcctgata ataaaaatgg
    12361 aataatcatt aataagagaa aacagaaacc accgttatgg gttagcgcac taaataaccg
    12421 ccatcgacca ctaataaatg cccgttgaca taattcgatg cctgacttgc gaggaatacg
    12481 gctgcgccca ttaaatcttg agtatcgccc caacggtttg ccggaatatg atcaagaacg
    12541 cgctgattgg tttctggatt actgcgtgtc gccagcgtaa tatctgttgc ataatagcca
    12601 ggggcgatac catttacctg aatattatat tgccctaatt catcacaata agctttggtg
    12661 aatccggcaa gagcatgttt agtggcagaa tatgcgggtg accattgtcc acctaagtaa
    12721 gagaacaatg aacagatatt aataatttta ccgcttttct gcgggatcat aatttttgcc
    12781 gcttcatagc ttaactcgaa tgcggcggtc aggttcacat caatcatcgg gtcccagtcg
    12841 gcacgaccga agtccagcac cttattcagc ttacaaatac cggcattgtt aaccagaata
    12901 tcaactgtac cgaaacgctc acagcaggca gcgataatct tctgcggcgc gccttctgcg
    12961 gtgatatcca cctgcatgaa gtccacctca acaccctgat tttcaatcat tttctttgtt
    13021 tcgccgttat ctttgacaaa acttggaata aagacatttg cgcctgcttt ggccaacgcc
    13081 atggcaaatg cctggcctaa accgctattc ccgccggtaa caattgcggt tttacctttc
    13141 agggagaaaa aatccattga gaacgcattg agagattcga ttgacatagt tagctccaat
    13201 ttccgtaagg caaaaaaaaa gaaaggtaac ttccccgcga gagggaagtt acctttctca
    13261 ttatctctgg taactggaga taatttttag cataaggaca taactgctaa tgtgatcgta
    13321 atcacagtgt gatattcgca ttatcaaata gttttaaaaa gcagaagcgt gattactctc
    13381 acacaacacc tctgctttcc atgttattta ttatttcaat tactagagac ctgagaaaag
    13441 taatccacct gaaagggcgg cttacttttc tctttttttt gtctattaca aatgggaata
    13501 cgatatgcaa cacaactcat atcgccgttg gataaccctc gcgataatta gttttagcgg
    13561 cggcgttagt ttcgacctgg cttatttacg ttatatttat caaattccca tggcgaaatt
    13621 tatgggattc agcaataccg agataggttt aataatgagt acctttggta ttgcggctat
    13681 tattctttat gcccccagcg gcgttattgc cgataaattt tcacaccgca aaatgattac
    13741 ttccgcgatg atcattaccg gattactggg tctgttaatg gcaacgtatc caccgctgtg
    13801 ggtaatgctc tgtattcagg tcgcctttgc gataacaacg attttaatgc tgtggtcggt
    13861 gtcgattaaa gccgcatcgt tgcttggcga tcatagcgag caagggaaaa ttatgggctg
    13921 gatggaaggg ctgcgcggcg tcggtgtaat gtcgctggcg gtgtttacca tgtgggtctt
    13981 ttctcgcttt gcaccggatg acagcgccag cctgaaaacg gtcataatta tctacagtgt
    14041 ggtttacatc ttgttgggga ttctgtgctg gttttttgtt agcgataaca acaacctgcg
    14101 cagtgccaat aacgaagaaa aacaatcatt ccagcttagc gacatcctgg ccgttttgcg
    14161 catcagcacc acctggtatt gcagcatggt gatttttggt gtcttcacca tctacgccat
    14221 tctgagttac tccaccaact atctgaccga aatgtatggc atgtcgctgg tggcggcgag
    14281 ctacatgggg attgtgatca acaaaatctt ccgcgcgctg tgcggcccac ttggcggcat
    14341 tatcaccacc tacagtaaag tgaaatcccc tacccgcgtg atccaaatcc tttccgtact
    14401 cggcctgctg gcgttaactg ccctgctcgt cacgaactct aacccgcaat cggtcgcgat
    14461 ggggattggc ctgattttac tgctgggatt cacctgttac gcctcacgcg ggctgtactg
    14521 ggcctgccct ggcgaagcaa gaacaccgtc ttacattatg ggcaccacgg taggtatttg
    14581 ttcggtgatt ggattcctgc cggatgtctt cgtttacccc attatcggcc actggcaaga
    14641 caccctgccc gcagcagaag cctaccgcaa tatgtggctg atgggcatgg cggcgctcgg
    14701 catggtgatc gtctttacct ttttgctgtt ccaaaaaatt cgtactgctg atatcgcccc
    14761 cacaacggct aacagcaagt aagccagaaa aattacagga gaaaaatcat gtcgaagaaa
    14821 tacatcatag ggattgatgg cggaagtcag agcacaaaag tggtgatgta cgatctggaa
    14881 ggtaacgtgg tttgcgaagg caaaggttta ttgcagccga tgcacacgcc agatgccgat
    14941 accgcagaac atcctgacga cgatttatgg gcatcattat gttttgccgg tcacgatttg
    15001 atgagtcagt ttgccgggaa taaagaagat attgtcggta ttggtctggg atccatccgt
    15061 tgctgccgtg cgttattgaa agccgatggc acgccagctg cgccattgat tagctggcag
    15121 gatgcacgcg taacgcgtcc ctacgaacac actaatcctg acgtggcata tgtcacctct
    15181 ttttcgggtt atctgacgca tcgcttaacc ggcgagttta aagacaatat cgccaactat
    15241 tttggtcagt ggccggtgga ttataagacc tgggcatgga gtgaagatgc tgcggtaatg
    15301 gataagttta atatcccccg tcatatgctg tttgatgtgc aaatgcctgg caccgtcctc
    15361 ggacatatca caccacaagc cgcactggcg acacatttcc cggcaggact gccggttgtt
    15421 tgtaccacca gtgataaacc ggtagaagct ctgggggccg gattactgga tgatgaaact
    15481 gcggtaattt ctcttggcac ttacatcgca ttgatgatga acggcaaagc cttgccgaaa
    15541 gatccggtgg cgtactggcc gattatgtct tctattccgg aaacattgct gtatgaaggg
    15601 tacggtatcc gtaaaggcat gtggacggtg agctggctac gcgacatgtt aggcgagtcg
    15661 ttaattcagg atgccaaagc acaggatctt tcaccggaag atttgctcaa caaaaaagct
    15721 tcttgtgtgc cgcctggctg taatggtctg atgacggtgc tggactggct gaccaacccg
    15781 tgggaaccct acaaacgtgg gattatgatc ggctttgatt ccagcatgga ttacgcatgg
    15841 atatatcgtt cgatactgga aagcgtggcg ctgacgctga agaacaatta cgacaatatg
    15901 tgtaatgaaa tgaatcactt tgcgaagcat gtaatcatta ctggcggcgg ttcgaacagc
    15961 gatctgttta tgcagatttt tgccgacgtg ttcaaccttc cggcacgacg taacgccatt
    16021 aacggttgtg caagtctggg ggcagcgatt aataccgcgg taggtctggg gctatacccg
    16081 gattacgcaa cggctgtcga taaaatggtt cgcgtgaaag atatctttat accgattgag
    16141 agcaatgcca aacgctacga cacgatgaat aaaggcattt tcaaagacct aaccaaacat
    16201 actgatgtga ttctgaaaaa atcgtatgaa gtgatgcatg gggaattggg aaatgcggat
    16261 tcgatccaga gctggtcgaa tgcgtaagtg aggaaggccg ggcgggaaac agcccggcct
    16321 gaacatacct gaatggttat ccccgctggc gcggggaaca taagttcgac aacttaaaaa
    16381 atgataacaa gtccggttta tccccgctga tgcagggaac ataataaaaa ctttaactag
    16441 ttcggtttat ccctgttttt acaaggaata ttaactacac tcgatgtctt tagaataaaa
    16501 gacaatacga atcgcacttt atcaattttc acttaaagta tgaactggat atctttattc
    16561 ataagaacta ttca
//
LOCUS       DAPGRN010000106        16108 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPGRN010000106 DAPGRN010000000
VERSION     DAPGRN010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.107"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..450)
                     /locus_tag="RHW33_003631"
     CDS             complement(<1..450)
                     /locus_tag="RHW33_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HDV1647098.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIALLKKIGLSKIAFALEDKIEVARVFRSH"
     gene            complement(524..1786)
                     /locus_tag="RHW33_003632"
     CDS             complement(524..1786)
                     /locus_tag="RHW33_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647099.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLEIEH
                     LKRQQQDAIEDATHDANQRADNAERISIELETKLNEMTAMVVLRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQIVDLNQQLKKAIKDASEARVAFANKKAEVTELV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHEMICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDELANKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            complement(2088..2789)
                     /locus_tag="RHW33_003633"
     CDS             complement(2088..2789)
                     /locus_tag="RHW33_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647100.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILENPPTCFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWIARCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            complement(2786..3463)
                     /locus_tag="RHW33_003634"
     CDS             complement(2786..3463)
                     /locus_tag="RHW33_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDV1647101.1"
                     /translation="MMAPTIYHRIDGTKYRNIWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILARALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            complement(3460..4086)
                     /locus_tag="RHW33_003635"
     CDS             complement(3460..4086)
                     /locus_tag="RHW33_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDV1647102.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGMEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSARERIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            complement(4588..4743)
                     /locus_tag="RHW33_003636"
     CDS             complement(4588..4743)
                     /locus_tag="RHW33_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HDV1647103.1"
                     /translation="MSSKVNYESLASVMPRNEQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            complement(4810..5388)
                     /locus_tag="RHW33_003637"
     CDS             complement(4810..5388)
                     /locus_tag="RHW33_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDV1647104.1"
                     /translation="MLRFTEEEFQAFSERRNKGRSRPKTKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRDGNCEHFEQVFIFDYFERKHPDIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLHAA"
     gene            complement(5391..5636)
                     /locus_tag="RHW33_003638"
     CDS             complement(5391..5636)
                     /locus_tag="RHW33_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647105.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NESIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            5783..6160
                     /locus_tag="RHW33_003639"
     CDS             5783..6160
                     /locus_tag="RHW33_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647106.1"
                     /translation="MATSITTTQSTRQYPLSRYDDRNIADPILRAELRKEVMLMCESN
                     DKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGLEDESQGMTVGKKLKLPPA
                     TWIREMIRHFQYDGQVIGTLSIA"
     gene            6170..7387
                     /locus_tag="RHW33_003640"
     CDS             6170..7387
                     /locus_tag="RHW33_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647107.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEEIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            7391..8119
                     /locus_tag="RHW33_003641"
     CDS             7391..8119
                     /locus_tag="RHW33_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647108.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            8106..8891
                     /locus_tag="RHW33_003642"
     CDS             8106..8891
                     /locus_tag="RHW33_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HDV1647109.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRTNQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHTANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            8893..9909
                     /locus_tag="RHW33_003643"
     CDS             8893..9909
                     /locus_tag="RHW33_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV1647110.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSSLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGTINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            9902..10534
                     /locus_tag="RHW33_003644"
     CDS             9902..10534
                     /locus_tag="RHW33_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDV1647111.1"
                     /translation="MGSLTGKYRAVVISVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEIRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVDKA"
     gene            complement(10581..11579)
                     /locus_tag="RHW33_003645"
     CDS             complement(10581..11579)
                     /locus_tag="RHW33_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647112.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFS
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDILRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALILTPPDCLPPDRL
                     ELVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFITQKPSRGNG"
     gene            complement(11579..12943)
                     /gene="dnaB"
                     /locus_tag="RHW33_003646"
     CDS             complement(11579..12943)
                     /gene="dnaB"
                     /locus_tag="RHW33_003646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV1647113.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIACILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQPSDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            complement(13230..13305)
                     /locus_tag="RHW33_003647"
     tRNA            complement(13230..13305)
                     /locus_tag="RHW33_003647"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13270..13272),aa:Ile,seq:cat)
     gene            complement(13333..14160)
                     /locus_tag="RHW33_003648"
     CDS             complement(13333..14160)
                     /locus_tag="RHW33_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV1647114.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            complement(15110..15185)
                     /locus_tag="RHW33_003649"
     tRNA            complement(15110..15185)
                     /locus_tag="RHW33_003649"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15150..15152),aa:Thr,seq:tgt)
     gene            complement(15188..15263)
                     /locus_tag="RHW33_003650"
     tRNA            complement(15188..15263)
                     /locus_tag="RHW33_003650"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15228..15230),aa:Asn,seq:gtt)
BASE COUNT         4164 a         4157 c         3704 g         4083 t
ORIGIN      
        1 gtgactcctg aaaacacgcg ccacttcaat cttatcttcc agcgcgaacg cgattttact
       61 tagaccaatt ttcttaagga gagcaatctt tgctgggccg tcatttctgt catcggtggc
      121 aggacgcata gatagcaaag gctcagcccc gtttgttacg tgcttacgca accaggctcg
      181 tgttttatcc ctggctatct cacagcgccc ggttacaaac cagaccgtgt aaacgttaaa
      241 taactggcgc accatatcaa taactggagt gatgggagca tcagtgtcac aggcaaggtt
      301 aaactcgttc cagtgctctg ttaatgcacc tttgccaggt ggtggaagca aatgcagcct
      361 gtcttccgtt gcctctgata tcgtcccatc aatatcgact atgacgatat acggacgttc
      421 ctggtgtgcg tgtttattga aaatactcaa atgccctcct cattggacga aaaaaatgct
      481 ggtgggacgc actccaccag cattaaaagt gacactgtaa ctatcagcga acgtaaatag
      541 tgccgccgtt ctctttttcc catgcatcgc tacgtgcata gcaaacatcg agaagtcttc
      601 ttgccgctgt ttcctctaaa cccaattcga caaccaactg ctcatgacgg cgggtaacca
      661 catcaaacag ggtatgcagc cctttattgg ccagttcatc aatgaattcc ggttcgaacg
      721 gtagctctgc atctgccaac ataacctctt gcgcccactc gacacggcgg accagttccg
      781 ggcggcggct ttccatctct ttacagatca tttcatggaa gaactctacc caaccttccg
      841 gctggaactc gcggaaaatg gccaacggct ggaagtttgg catcaaccat tcgttgatac
      901 gtatatcaat ggcatagccc atgtcgcagc agaactgata agcaaagtcc agcttagaaa
      961 cgatataagg acgctcgtta ttgaactctt taggcgatga gatcccataa gccaggaggc
     1021 gcgggaagaa tgagatttgc cctaacgtcg gatggagttt gcttgcaggg aaacggcgct
     1081 cagtaatgcc atacatttcc ttcttgagcg tcgcaaattt ggcgttttca ttaaccagct
     1141 cggtaacctc tgctttttta ttagcaaatg ccacgcgcgc ctcgcttgca tctttaatag
     1201 cctttttgag ctgttggtta aggtcgacaa tctgcttacg cagttcctgt cgctcgcttt
     1261 tggctttgtt atagcgtttc tcaaggttaa aaggatcaag tttcatgatc tctttatatt
     1321 gagattttag agttgaaatc tgtgagttcc gcagcacaac catcgcggtc atttcattga
     1381 gttttgtttc cagctcaatg cttatacgtt cggcattatc agcacgctgg ttggcgtcat
     1441 gcgtcgcatc ttcgatcgcg tcctgttgct ggcgtttcaa atgttcaatt tccagctgaa
     1501 gctcttcaat ttctttaccc ttcagaccga gatccaactg catattttca gctgcatcta
     1561 ccagggagtt atggctatca gcttctgcgt tataaacatc aataagctgt gcgtgaagca
     1621 tctccgctga ctgaaccgca ttatcaaaaa aacgcgctgt gaggtcatca caactaacgc
     1681 ggcgttgcgc ggctcggatg ttctggataa tggccgggat accggcattc aggacatcag
     1741 ggatagaaac attttcgatt gattggtttt gtgctgaagt gctcatttca aagttccgta
     1801 ttagcttgtg cttcggtcat ttttcctaag tatgaaggtg gaaggactac gcaatttgta
     1861 tccagtccct cacctatggc agcctgtaaa attctggcta aggtgagtct cttgttgcga
     1921 tacctggtga tgacatgcct gataccgccg gtcggcgtaa caaaggcgat cagccagtag
     1981 tgatatttcc gtcggaatgg ccacatagtg caccttgtag attgctctaa taaaaaacgt
     2041 gatgagtgta catcacgttt taaaaatatg gaattattag agcaatatta ttctgattct
     2101 cgctcaaaaa acgagctaat gaggggaagc caatcctctg acacttcgcg aggccgcggt
     2161 ttgccgtgga aaaagattat tcggcagtcc tttggtaatg ccccattccc cctggagtaa
     2221 cgcgcgctcg catattttga accaggttcc acaacatcgg ccttgtaact tacaaaccat
     2281 cctggataca gatcctgaaa tgctggtgta tcatcgccca taaccttccg taagaatccc
     2341 tgatcacccc agcactcagt agtgacacaa cgagcaatcc aaccttccgg atcttgccag
     2401 aatgaactcc agatatgtgc ttttacacta tttggtatcc acagggcacc gctaccacga
     2461 tattgtggat ggtaaaaatc cctaagcatg gtgaagcagg ttggtggatt ctcaaggatt
     2521 gggcgtatat caccggcaat aaccgtgtcc aaatccagat agaacagatc atcggttata
     2581 tccggtcgga acaactcgat tttcgcccac cagccacggc acttttgcca ctggttgatc
     2641 aatgggacaa ctttgacgcc aggtacatgt aaacgcttca ggtctgtcag gcaaataatt
     2701 tcatagcctt ttggcagttg attaaccagc cactgcacat cggaagcgtt atagtcacca
     2761 ccagagcgaa gaactaaagc aatcttcatg ctgcaccatc acctttcact ttcatcaatg
     2821 tcaggtttcc gcaaaatacg gcaccagtgt cgatatactg ctgattccag aatgtcttcg
     2881 ggcttttcac cggagtgtga ccaaagataa aacgatctgc gcccgaaatt tcgccaccaa
     2941 tatcatccat cgaatcactg atacgctcgc gcgcccagac aacgttgaaa agcggcacct
     3001 ccttaccgaa ttggtattca ttatccggat agtcggcatg ggctataacg atagtttctt
     3061 gcccggtgtt caactcaatg atatagggca gacgctttac cagctccacc agcgccctgg
     3121 ctaatatttc ctgatcagtg tccagcatga agaaccattg tccgccattc attagccagt
     3181 tattcacgtt gccatcagga cttaacgcat caatcatcag ccgctcatgg ttccccatca
     3241 ctgccctgaa ccagggcatc tgcaatagtt ccagacattc gacattttca gtaccgcgat
     3301 cgataaggtc gccgaccgat atcagtaaat cctgcgccgg gtcaaaatcc acacgatgga
     3361 gttcggacat cagtctggtg tagcaaccat gcagatcacc aacaacccag atattcctgt
     3421 atttggtacc gtcgatacgg tgataaattg ttggtgccat catgtattct tcagccattc
     3481 tttaagagtc atctgcggaa tacctcccat tttcccgcat gaaacaacgt caatccgttc
     3541 acgcgcagac tggaataaca aaggcaggtg acttagattt tttggcgtgc cgccggagtg
     3601 aacgcgtggt tcttgtgtag cgtcaacgcc caccagggcg acatgtttga atccgatatg
     3661 gaaagccagg ttcagagcac catatgcact attgccgctg gcaatttcat tctcatcttc
     3721 gcaaagtccg aaatgtgcgg accagcgcca cgcccaccac tcgggagaat tcgtattttt
     3781 tggctccatg ccacgttcag ccacacgacg gaagcacaga acgccgtctc tgacttcacg
     3841 ttctttaaca tcgggtagtg ccatgcaata acaaacacca cggcgacggc ggccacgacc
     3901 aacgcgccgc atattgtctg gcgatggatc aagtgtgaaa aaataagaag cgcggttcag
     3961 ccagtcgatg gccccattga ccgctataat cggcactccg cgcggcgcaa caaagtttgc
     4021 ggcgcttggg ccactgccga cgataataac gcgatcactg cctctaaatt tattcttggg
     4081 aaacattgaa ttgcactgct cctacttgca ttcaaaatat gtaaatctgc gtgttttttg
     4141 cgggtatcca ggaactgctg ttgccatttt gaaatagaca cctgcgttgg attccgtagg
     4201 gcttgagggt gcgcgccatg ccaatgaagg ccgttttgca gagaacagtc atagccgact
     4261 aataccacta cttcagcccc tgattcagca gccagactga tagcctgcgc gccgctattt
     4321 accccttccg ccggtccaca atatcgcctg tactccaacg aaaatgattt cgccgccgcc
     4381 aggttggctg tcactttgcg gaatctccct cccggtatgg tggatccgta ttgcttccac
     4441 catgacaaat caccggcgta taaagcataa atgtcatcga acatctgcca ggaattgtta
     4501 accgcgatga ttgaacagcc agttttttct atagcagcac agtcctcacg agtgagtgac
     4561 ggaccgctac cgacacaaaa aacagtccta gtcgccctgg gtggtatgtt cattctcagc
     4621 tgcaaattca gcctccaggc gagcattcat ttcagcgatt acagggtcca ctacagcatc
     4681 tgtttcctgt tcattacgcg gcatgaccga tgccagcgac tcataattaa ccttggatga
     4741 cacgattatt ctcccgatgt taaagtgcac taccacaaag agcgtacatg cactaattaa
     4801 tttattattt taagcagcat gcaaccactt atcgccgttc aatacatgct caatagcctc
     4861 acccttttta agacttatgt attccaggat ggcggtaatc gcttgttctg caccatacgc
     4921 aagaacaacg tagtagcctt cctctctaag cctgcgcatc caggcgatct gctctttcgt
     4981 cggggcttta ccatttggtt ctttaagctc aattcgcatg ccgtgataaa taccgcatgc
     5041 tttatcgaga ctcatgtccg gataaccttt tttctgccct tcagccttca ttttcccggc
     5101 ggttgctttt gaacgtttcc ctccgttagg cgttgcatgc aacagctcat agatgtcagg
     5161 gtgcttgcgt tcgaagtaat caaaaatgaa aacctgctcg aagtgctcgc aatttccgtc
     5221 gcgcaggtct gggttctttg caagtgctgc aagtgccttc gcatgtggag aaacttcttt
     5281 taccggcgca agcgataaga atggatcctt tttggttttt ggcctggacc gccccttatt
     5341 tcgacgctca ctaaaagcct gaaactcttc ctcagtaaag cgcaacataa tcagtcaaat
     5401 cctgccggtc gcatgccata tttacgctgt tttgcggcct gctcttccct gtgccattgc
     5461 gcacattcag cgtcacaata aatgcctgat tcaatcgatt cattgcagta acgacacttc
     5521 cctgtaaata cctggctcac gacctgtgcc tgctttctga tgttatcgat ggccatgtct
     5581 ttgagagctt ctaactgatt catgctcagc tctgcatcat caacacgttc tgccaatttt
     5641 gtttcctcgt gaagaaccta cttaagggca gaatgataca tttcacaatc aaaattgcac
     5701 taataatttt cttttattga gttaaatatt caacaaatga ctagcggtag aatcaccatc
     5761 atctatttct ggcaggctga ctatggctac atcaatcact acaacccaaa gcacccggca
     5821 atatcctctg tcgcggtatg acgaccgcaa catagccgat ccaatactca gggcagagct
     5881 acgcaaagag gtgatgctta tgtgtgaatc gaacgacaag aatctgacga tttattacgt
     5941 tcttcccgat gagcaatatc gcccggattt gctggcttac cgtatgtggg gcatagcaga
     6001 gctacgctgg gttgtgacgc tcgccgccgg gcttgaggat gagtctcagg gtatgactgt
     6061 tggcaaaaaa ttaaaactcc cacctgccac ctggatccgc gaaatgattc gccatttcca
     6121 atacgacggc caggtaatag ggacattatc cattgcgtaa gggaaatgaa tgccaactga
     6181 atatgctcgc gacaaccttg gtcgctatca gactgatgga ttaagtgcaa aagactttaa
     6241 caaggtcttc gatcttatcc gtaaacagca gcgtcagaat cggcgaaacg cgcgacgtac
     6301 actcacccca aggattatgg ggatgcgtaa ccgcgaactt gaggcattcc tcagccttgg
     6361 gaaaaagaaa gatggcacct actttacgcc cgaagatata cgcagtttca acacctcaag
     6421 gcaggctcat aaaaccaaat tcaagagcac ggtacccggc attacctatg ctcagctggt
     6481 ggcgcagtcc accagcattg atataaaacg cgctaacaac aaggtttctg atggcacagg
     6541 gatcaaagcc gcgacattcc tcgggctaaa acacaacctt gcattgatat ctgttaatgc
     6601 ctcggatgag tcggtccacc agcatcaccg tgtcagaatt cgatttgagg aatgggataa
     6661 agccgttgag gaaattgctg aagacggtgc gaaaaaagcc cgaatcgctg ccgatctctg
     6721 caagggccgg gtatctttcg actgtgattg tggacgccat caatactggt atcgttatat
     6781 ggccactgct ggtaactatg ctgtcgcgcc gccaaaagag tatgcattcc ccaagatccg
     6841 caaccctgat ctgactggtg tagcctgcaa acatgtgttg cacgctatga cgcgttttca
     6901 gtctcccaca tggcacaagg ccatcattat tgccctggaa aaagcagctg aacaggtagc
     6961 cttcggcgat gacaagcgga agacaacaac ctatttcaaa ggcgaactgg ctaaatcgct
     7021 cgcgcgcaac cggacaacaa cgacggatca ggctaaagcg gcgcgtgagt atgagttata
     7081 tctgaaatct caggatgcat taggcaaaaa actacgcgcc aaagatagcg ccacggacaa
     7141 cgttcgccgg ttgttaaaaa aagctcgcac cacggcaaac aggaagaatg ccgaactaaa
     7201 agcatcgcgg gtgagggaag cccaggctcg cgctgaagcc gacgctctca aaaaagccct
     7261 gcaaacgcag gcgaacaacc tcataaagtt tttcatgagt cagggaatgg acaaggccgc
     7321 tgccactgcg caggcgcgaa gcattcttga gacacaaatt aacgaagccc gtaaacggaa
     7381 aggataatcg atggctggtt tctttgatga catgtttgag gacacagaac catcacaaca
     7441 agtgactggt gataacctcc cggacaccga atcggatccg gatattccag gcgaaggttc
     7501 tgaactgatt gaagaggaag atattgatgc tgaaatcgaa accgatggtg ttaacgttgg
     7561 taatattgtt gatcctgtgg aggacaatca ccttcccaat ctggatcacg gcctgcttag
     7621 tgattctggt gtgcgccacc gttatcaagg tcatgcagtt tttaataacc ttgtgcggat
     7681 ggactggctc aaagcaatca agctagaccc tgactcattc gatgcggttc tgtatcgcgc
     7741 aataccttac agagacaaaa atgcacctga aacggcatct gaaataatag aaccgaacca
     7801 acgcatatat gactatcagg atccagaact gataacagcc ctcgactgcc cggatgagat
     7861 ggacgccttc tacgcgctat acgacggcag tgataatacg ggaattagcg acagtgcttt
     7921 aatccttcgg ttggccgccg ttaatgtgcc agtgggttct atgctcgaat ggctggaaca
     7981 gctgtcagac ggcacaacca ttcgccgctt ctggtacatc cataaaatat tcaattacgg
     8041 cactgccagg gtaggcagtt tgttttattg cgtgccttca cgcgcctttg aagggaattt
     8101 catcggtgat tctgaataat caggaatggc tactggccat ctttaagaaa aaaggtctta
     8161 ctccaaccgg taagctggaa tttgccacta ttgatggcat tgattcggcg ctcgcacagg
     8221 ctttaaacga agcattcgac tcacaagttg tcagctttaa tgatcgcact aaccagtcat
     8281 tcagggagtt cctgaaacgc acaccaagag atcgcataac gctcggcact tttagtgatg
     8341 tgaaggagtg gttgtcgtca tttgaagccg atcgcgccgg gcgcaaagat acagcctctg
     8401 ctggcccggt aaataagctg gcaatgccgc ttgtgaatct gtctcgttct cccgcatttt
     8461 caatttatga aggtgaactg tgccgggata attacgatga agggcatgtc accaatgaaa
     8521 atgatgagat tgaagccctg gtatcgacta tccctttctc actggaatat tcgctatgga
     8581 tagccagtga cgagaaggaa tctcttggga tggttacaac tgcattagca ttctggctac
     8641 gaatgtatgc cagcctcggg caggcatctt tcactcacac tgccaatgtc ggcggttatg
     8701 agataccggt tacctgttac atagaagggc aaaaatcaat cgcatttcag gatctgacca
     8761 ccggcaccgc cgacaacagg ctgttcgcgg ttggattgaa cctcacagta gtggcggagc
     8821 ttcctatcct ggcttatatg cagcaaacca ccggcaccat aacggtaaaa gcgaaaattc
     8881 tggaggaatg agatggccac aaagaccacc acagccccgg aaactgattc aaaacgcact
     8941 cagctattcc tgcaatctgt ttcaattggg cagaacgaaa tccctcgcga aatgatcgta
     9001 ggatgtacct atgtcgaacc cggggagcta tctggtcccc agcttatgct catggtcagg
     9061 gattcaacgg cttacgtggt caataagctg ggggtgaaat ttgggacaat actgaccgtt
     9121 tcacttggtg atccggaagg tcatggcggc atcctcttct cggaagagtt ctttgttctt
     9181 aaagcgccgc gcaaggacga tactgtactg atttacgcgt ttagtaaccc ggtgcggtta
     9241 ttaaaagttc cgtccaccag cgcacagtat tttgttgata agcccccatc agccgtagtt
     9301 tcctctcttg cccctggtct gaaggtaaat gctgactcat tcagaaaaac atccacatac
     9361 cacctaaatg ttggagaaaa accgaccaag gtattgcagg agatagcccg ggataccggt
     9421 tctatgtgct gggcatccag ggggacgatc aattttaaaa gtatggaaaa aatggcaaac
     9481 gccgctccat cgcttactta tgagtccgcc aatcccaaca catccggatt tacaattagt
     9541 cagttcaaca tcctgaatgc cgattatgaa taccagcgcc gccacaatta cagaatggcc
     9601 agttatgaca tgaccaaagg tgtggtttac tcaggtaacc aggaagaccc cattaaattt
     9661 acgagcaatc ccgatcctac cgcgctggcg aactacaaca aattcattct cccccgcctc
     9721 gatatgctgg tggaaggaaa tgccgcgcta actccgggta cgacgctgaa aattgtcgtg
     9781 cataacacgg caggtgacgg agaactcgat gaatcaatcc ctgacaaaat gatagtgatg
     9841 tccgtgactc atttcgaaga ccgctttcgt tttgtcagcc gtgcacagtt aggagtggtg
     9901 aatgggtagt ttgacaggga agtatcgggc tgtagtgata agcgtcgatg accctaaagg
     9961 tctgatgcgt acacaaatac gtgttgtcgg catgatggat gggttaccag atgcctcatt
    10021 gccgtgggca gaagctatat tgtccaatgc aaacacgttt tcaccatttc tgcccggcga
    10081 taaagtatgg gtagaatttc cctacaatgg ggattcgcga tggccattga taatcggtta
    10141 tgcacaggat gcatccggtg gcgctcccaa tgtgccgcct gaagcgtcag gacaaggtga
    10201 aggctatgta ccgcctgaag ttgaaggtgc accagcacaa ccatcaacca gcgccaaaaa
    10261 agactttatt tcgtcgcgga acggactaat ggagatccgg acggcgggcg gagcctgggc
    10321 cgttacgcac ttgaaaagtg gaacaacaat cgggttcaac gaggccgggg agttatatgc
    10381 catttctcaa ggtccggcat tcatctcttc cgcaggaaat ctcgatataa agtcaggcgc
    10441 ggatgtcgcc ctgaaggcgg ggggaagtat ggcgatagag gccagcggga atctatccat
    10501 aaaagccgct caagtctctg ttgacaaggc ttaagaaaag cccggcgatc gggcttttct
    10561 gttatgacgg gttcaatttt ttatccgtta ccgcgcgacg gtttctgcgt gataaacgtc
    10621 tcaagcatct tttccgcaat tgccgaccag gtgtgacact ggaccttttc agcatttttc
    10681 acgcgatcaa cgcgagcaat aacctcatcc caatcaatcc gcgacttgat aaccatatgg
    10741 ttcaccaact ccaggcgatc cggcggaagg caatcgggcg gagttaatat caacgctcca
    10801 cacattgccg cctcaagaac agttaatcca aggctttcgg gatgcgtaac gataaaaacg
    10861 tcactcttac gcaattcagc tgcaaattcg gttgctggca ccggcgtccg cctgtatgga
    10921 gttaccgaaa tattccccgg atcaatggta accaatccgt catcagtcaa cgttctggcc
    10981 tcatacggaa cggtcagacg ctgaaggttc ataaggatac ttaaggagtg atcaaaacca
    11041 ctaacatcaa atgcagcgtg gtctacaaaa atacgcagaa tatcgtctgt tttggtttcc
    11101 agatggaaca gctcctgatt cgctgcccat ccaacatgtt tgttaaaacg attatgacgc
    11161 tctaacctgc cgggattatc caggtaccgc caggtatcat cgcggacagt aaaagtaata
    11221 tcgactggtg ccgaatccag catagaaccg tcatatacct gggctaccca tccagagaat
    11281 cggcgacaca gttgcatgcc tatttccctg ggtaccgtag taaaatacct caatcctggt
    11341 gccaaaatgg ccttcgcaga acatgcggtc gcagcagtca acacagcttc aacataatcc
    11401 tccgggcttt cgacgccagg ggaatatgga cgatggtatt gcaatgttac ccctgcctca
    11461 ctaaaggcgc aggccaggtt ataagcccac atttccgtat atgttttcac atcactgata
    11521 gcttcaaatt ttcgcccaat gatcaggatg ttcatcggct tttcctcatt ccattgcatt
    11581 aataatcctc ttgccagtca gcaccagcat agttatcaaa ccgtgagtat tggccgttaa
    11641 aagccaatct caccgtgcca attgggccat ttcgttgctt tccgataatt acctcggcaa
    11701 tgcccttcat ttcgctatcc gggtgataaa cttcgtcgcg atacagaaac atgatcaggt
    11761 ctgcgtcctg ctcaattgct cctgattcac gtaaatctga atttaccggt cgtttgtccg
    11821 cacgctgttc aagcgatcga ttaagttgtg acaatgccac caccggtact tgtaattcct
    11881 tcgccaacgc cttcagtgag cgagaaatct cggcaatttc cagcgttcgg ttatcttgca
    11941 gctcggggac gcgcataagt tgcaggtagt cgatcataat catgctcaaa ccaccatttt
    12001 ctttataaac acgacgagcg cgggaacgaa gctctgtagg tgtcagggcg cttgagtcat
    12061 caataaaaat attctgcttg tccaacagaa tacccattgc gccagaaacc cgcgcccaat
    12121 cctcgtcgtt aagttgccct gtccgaatac gagtctgatc aacgcgtgca agagaagcca
    12181 gtgagcgcat catcagctgg tggctcggca tctcaaggct aaaaaccaat acgggcttat
    12241 cgttacgaac tgcggcattt tcgacgagat tcatcgcaaa cgtggtcttc cccatagatg
    12301 ggcgggcggc gacaatgatg agatcggacg gctgaagccc tgccgtcttc ttattgagat
    12361 cggtaaatcc cgtatcaagc cccgttacac catcatgtgg tcgctgaaac aactcttcta
    12421 tgcgagatac cgttgcatcg agaatgcagg cgatatcttt tggaccacta ccgctctttt
    12481 gtcgtttttc agctatttca aaaacgcggc gctcggccat atctagcaat tcattgctgc
    12541 ccctgccatc ctgcgcatat ccagcttcgg ctatttcatt tgcgacggaa atcatttcac
    12601 gaacaaccgc gcgttcacga acgatatccg cataagcaca aatatttgcc gcgctgggcg
    12661 tgttctttga catctccgca aggtacgcaa aaccaccggc gcgttctaat ttaccgttct
    12721 gttcaagtgc ttcagcaagt gttatcaaat caatcggttt gccatgactt aataacctct
    12781 ccatctcact gaaaatttca cgatgagcac tggtataaaa atcatcagca actatacgat
    12841 ctgcaacttc atcccagcgg cagttatcaa gcattaagcc accaagtaca gcttgttctg
    12901 cactaaggga atttggcatg gattcaagag gggatgcaga cattagcact ccacccaggc
    12961 gtgctgaatg tcagatataa tcggcatact caaatcactc ctaacgatat gagtcatcac
    13021 cagaaaatca ggattaatgc gccggactct tcccggctgt cacaccgaat cgccaggatg
    13081 gtgaatccct ttacccgaga aacaacaaac ggtggcttgc acattccggc tacctggttc
    13141 gttgcctgag ctaggggcaa ggttcccccc ttttaacgtc accagaccgc taacgacgca
    13201 tgtgccagac gccgtgttac aaccaaatat ggtggcccct accggacttg aaccggtgac
    13261 cgtgcgatta tgagtcgcca gctctaacca ctgagctaaa gggccggatt actgccaatt
    13321 ttgcttacgc ttttatttca ccggaacaaa cggaacagcg gtattactgg tcatatactg
    13381 cggtaatgta ccgttccatt tgttgatcgc ttccaactcc ataacaccgg ggttctggcg
    13441 cagagcttca ccacgtaaac gaatggcatc agcttcggcc tgggcttttg tgcgaatagc
    13501 atcagcctgt ccggcagctt ccgcgcgcag catgttggcc tctgcttcac gttgtttgac
    13561 ctcttgctcg cgttgcaggg ttttttggtt tgccgtgact ttggcgttaa tactgtcaat
    13621 aactgttggc gggtattctg gcttacctac ataagagagg ctcatcacct gaatgccgat
    13681 tggcgtcatt tcttcctgaa tgtctttaag ggctgcatca agcaattcag atttgccacc
    13741 gtcgataaat ttgtcggtgg tcattttgct ggctaaccgg ttcagagcat ctgcaacctt
    13801 ctggcgtaga tcggtatcag taatatcatc tacacctttg cgataggtct gaaataccgt
    13861 tgtgactttt gctggatcaa ccttgtaggc tacgccgatg tggtaaccaa tggttgttcc
    13921 gtcgctcatc tggaagctga acggctcatc gtatgtcttc atttgcttaa aggtcgggaa
    13981 gatataaact tcagtattca agcctgtcca gtagcgacca acgccaacta cttcaccgat
    14041 acctttatca tcccccagct tatttacttt gatccctacg ttacctggct ctacccgatc
    14101 gcatccggtc agacataaag aacccaaaat aatcgctgca ctaatcaacg tttttttcat
    14161 taattaattt cctggttttt tcacgaaaaa agactactgc gaaagccggg taaatgagcg
    14221 cgagaaggac tcccaacaat acaagtattg tgctgttaga tgagatcata tttggcaaaa
    14281 gccaaacata cagaaccagt gacacaatca aacagaggac ggcataaata tataaccgca
    14341 cccatagcgt tcgacatttg ttcggattgt tctgcatcct ctcactccat tatttaacga
    14401 ataaaaaagc tgcggtgccg ggtgcctccc ggtgtccttt ggctggttat ccaccgtgga
    14461 cggggaaaca aggagaaatg aatggactga tataaccatt tccccgcgtg cgcttagccg
    14521 cattcaccgc aacggaaaga gcattcttgg tggacctgta gattgggata tgaacccgtt
    14581 acaggagaat gctcttacct gttacgtgct ccgtttcgtg gagctaacgg cgggtgatcg
    14641 ggccgcacca ggctggactt atttcagcgt tatgctcatg ccagagaatc aaactgtgat
    14701 ggtcggtgct gaactccgac acagggttgt agcaagcccc gcaaagcgcg cactactgta
    14761 gttgcggcac atcagcctgt gcattcacca caatgttgag aacactggtt gtcacgctgc
    14821 aacgcaacat ttattcgtag attgggatat gaccccgtta cgccagtgtt ctcaacgttg
    14881 tagtgccggt tacggttccg gccaggcctc ttcctcaacg gggtgttctc catacggact
    14941 accgtttatt ggtcgttcct gcggtttatg ttgtgaagcc agatgcttat cttctggttg
    15001 cttcaaagag ctgcacttca tcacaacggt aagggtactt cgtagggatt cgaaccctct
    15061 gccaagctcg gcgatctccg acgtcgcaaa atacccttac ctgttgtgct ggtgccgatt
    15121 aacggactcg aaccgctgac atcctgctta caaggcaggc gctctaccaa ctgagctaaa
    15181 ccggcattgg cgatggtgga tggatttgaa ccatcgaccc gttgattaac agtcaaccgc
    15241 tctaaccgct gagctacacc atcacttgcc gggtacgtct ccggcgaggg cttccacctc
    15301 cgtatgcttt tcggcgcacc gcgccctggc tgcaattcgg taacagggga tgcacaaccc
    15361 tggcttccag cgtgattagc gccttcagca tgacgggata tacccgtaaa ttcgtggaac
    15421 tgtacccaaa gtgctgttaa gcaccgctgt tacgctgaaa agaaaacgca acaggaaagg
    15481 acgctgacca acagatggcc ccttctcgtt catctggtta atcacaccag cgcccttacc
    15541 tgttgtgcct ccccgttccc taatacacag acggggacac tctgcggtcg atttttttga
    15601 cgggggacga ctcatacccc gtggcatctg gtttcttagg ccgctaccat catcagatca
    15661 tcgtttgcat ttactttaat ggtcagtttc taaaccgccg caaagtcgct aaccatgacg
    15721 aaaaccctga aaaaaacgcc cacccgaaga tgggcaaact ggaagctcgt aacgcacttc
    15781 ggcgttgcca cttaggcgca tggtcaacct ggcaactcgg tggtttgtct gggaggacta
    15841 ggcccagcca tgcttaccgc cgcgcctgtc gcggctaaca gctaaatcgc tctataaatc
    15901 acgattcatt gaggcgatat tacactaata aatttattag agcaatatac ccaaaacgtc
    15961 atgagctaca cctcgagtgt cccccttaca agacacagaa cgtctggcaa aaagatgttc
    16021 cactctgaag ccactgtcat gataaagctc tctgatgttt ggcgcgccac tgttagtaat
    16081 gagaaccttt gcacctcgac gatgagca
//
LOCUS       DAPGRN010000107        15947 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPGRN010000107 DAPGRN010000000
VERSION     DAPGRN010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.108"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            179..703
                     /gene="lpfA1"
                     /locus_tag="RHW33_003651"
     CDS             179..703
                     /gene="lpfA1"
                     /locus_tag="RHW33_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA1"
                     /protein_id="HDV1647115.1"
                     /translation="MKKVVFALTALALTSGSAFAAESGDGTVKFTGEIVDSPCVLSVD
                     SQNQEVVLGQVQKSVFAAVGDKSPAKPFEIKLEDCDTTTMKKANVSFSGVGDADQSDL
                     ISVSTEAGAAKGVGIGIYDNSNTLVALNGGKASVDLSKGQTVLYFTANYVSTLATVTT
                     GYGNAQVDFNLSYE"
     gene            768..1454
                     /gene="lpfB"
                     /locus_tag="RHW33_003652"
     CDS             768..1454
                     /gene="lpfB"
                     /locus_tag="RHW33_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312457.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HDV1647116.1"
                     /translation="MKSKFVALALSLFLSQSVLAGGVGLSATRVIYDGSKKEASLTVQ
                     NKSKTEEFLIQSWVDDAAGSKKTPFIITPPLFRLDPGKNNILRIVNTTPGLPQDRESV
                     YWVNVKAIPSKSDDSENKNVLQIAVRTRIKLFYRPAGLQGDVKTAPNELRFTRSGNQI
                     RVDNPTVFNITFNQFFANDKEIEKAGMVPAKGSLNITLPAGAGSVSKIKYNTINDFGS
                     SAEMMTKNVD"
     gene            1483..4056
                     /gene="lpfC"
                     /locus_tag="RHW33_003653"
     CDS             1483..4056
                     /gene="lpfC"
                     /locus_tag="RHW33_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HDV1647117.1"
                     /translation="MSRKTVSRTFSSFSISVVAVAVASTFSAHAGKFNPKFLEDVQGV
                     GQHVDLTMFEKGQEQQLPGIYRVSVYVNEQRMETRTLEFKEATEAQRKAMGESLVPCL
                     SRTQLAEMGVRVESFPALNLVPAEACVPFDEIIPQASSHFDFSEQKLVLSFPQAAMHQ
                     VARGTVPESLWDEGIPALLLDYSFSGSNSEYDSTGSSSSYVDDNGTVHHDDGKDTLKS
                     DSYYLNLRSGLNLGAWRLRNYSTWSHSGGKAQWDNIGTSLSRAIIPFKAQLTMGDTAT
                     AGDIFDSVQMRGAMLASDEEMLPDSQRGFAPIVRGIAKSNAEVSIEQNGYVIYRTYVQ
                     PGAFEINDLYPTANSGDLTVIIKEADGSEQRFIQPFSSVPIFQREGHLKYSFAAGEYQ
                     AGNYDSGSPRFGQLDLIYGLPWGMTAYGGVLISNNYNAFALGIGKNFGYIGAISIDVT
                     QAKSELNNDRDSQGQSYRFLYSKSFESGTDFRLAGYRYSTSGFYTFQEATDVRSDADS
                     DYNRYHKRSEIQGNLTQQLGAYGSVYLNLTQQDYWNDAGKQNTVSAGYNGRIGKVSYS
                     IAYSWNKSPEWDESDRLWSFNISVPLGRAWSNYRVTTDQDGRTNQQVGVSGTLLEDRN
                     LSYSVQEGYASNGVGNSGNANVGYQGGSGNVNVGYSYGKDYRQLNYSVRGGVIVHSEG
                     VTLSQPLGETMTLISVPGARNARVVNNGGVQVDWMGNAIVPYAMPYRENEISLRSDSL
                     GDDVDVENAFQKVVPTRGAIVRARFDTRVGYRVLMTLLRSAGSPVPFGATATLITDKQ
                     NEVSSIVGEEGQLYISGMPEEGRVLIKWGNDASQQCVAPYKLSLELKQGGIVPVSANC
                     Q"
     gene            4071..5132
                     /locus_tag="RHW33_003654"
     CDS             4071..5132
                     /locus_tag="RHW33_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1647118.1"
                     /translation="MKAAIALSLLGCVFGFSGKALAGGSWGPCTPVDGTTYHYNVDVD
                     VGVPDASKNVAGTVLTDVLNWSNGQNVSIICECPDKGDYKIEKDTLIQGVSGIANMTR
                     DIGGFKYYYLTDELSVSTKIQVSSGQYGFVPFKGQQALQVTGCNEVITTPYMGGGGLL
                     SFLITKPFIGDSVIPPTLIAELYASKTNKDYGTMPISSVSIQGRVTITQDCEIKPGTV
                     LDVPFGEFPSSAFKNRQGQMPEGATEQEINLGFDCNNISDGIKVSLRLEGATNADDPR
                     AVDMGNPDIGVLVEDSSGKILVPNDASSTTLLNLSSLDSKTHRNAAIRLLALPISTTG
                     KAPKGGTFEGVTTIYLEME"
     gene            5137..5667
                     /gene="lpfE"
                     /locus_tag="RHW33_003655"
     CDS             5137..5667
                     /gene="lpfE"
                     /locus_tag="RHW33_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HDV1647119.1"
                     /translation="MKFNLLLHSGIASLSLFACGVNAATDLGPAGDIHFSITITTKAC
                     EMEKSDLEVDMGTMTLQKPAAVGTVLSKKDFTIELKECDGISKATVEMDSQSDSDDDS
                     MFALEAGGATGVALKIEDDKGTQQVPKGSSGTPIEWAIDGETTSLHYQASYVVVNTQA
                     TGGTANALVNFSITYE"
     gene            5922..7613
                     /gene="eptB"
                     /locus_tag="RHW33_003656"
     CDS             5922..7613
                     /gene="eptB"
                     /locus_tag="RHW33_003656"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV1647120.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVNDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPAEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKAADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAVAN"
     gene            7705..7781
                     /locus_tag="RHW33_003657"
     tRNA            7705..7781
                     /locus_tag="RHW33_003657"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7739..7741,aa:Pro,seq:cgg)
     gene            8534..10141
                     /gene="dppA"
                     /locus_tag="RHW33_003658"
     CDS             8534..10141
                     /gene="dppA"
                     /locus_tag="RHW33_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV1647121.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10292..11311
                     /gene="dppB"
                     /locus_tag="RHW33_003659"
     CDS             10292..11311
                     /gene="dppB"
                     /locus_tag="RHW33_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV1647122.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRLRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11321..12223
                     /gene="dppC"
                     /locus_tag="RHW33_003660"
     CDS             11321..12223
                     /gene="dppC"
                     /locus_tag="RHW33_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV1647123.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12234..13217
                     /gene="dppD"
                     /locus_tag="RHW33_003661"
     CDS             12234..13217
                     /gene="dppD"
                     /locus_tag="RHW33_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV1647124.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13214..14218
                     /gene="dppF"
                     /locus_tag="RHW33_003662"
     CDS             13214..14218
                     /gene="dppF"
                     /locus_tag="RHW33_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV1647125.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14248..15519)
                     /locus_tag="RHW33_003663"
     CDS             complement(14248..15519)
                     /locus_tag="RHW33_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1647126.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3960 a         3860 c         4245 g         3882 t
ORIGIN      
        1 tatcccgaaa ggcaacttag tgatttaaat caaaattcac actttcttaa tttattgata
       61 ttttatcctc ttaattttct tttccttgtt tatttgtata atcccgcctt caaagttgat
      121 gcaatgttaa agcatcacta tttgctaatt aatgtgattt aataaaatgg agtttttcat
      181 gaaaaaggtc gtttttgcct taactgcact ggcgttaacc tccggctctg catttgcagc
      241 agaatctggt gatggcacgg ttaaatttac aggcgagatc gtggattcac cttgcgtact
      301 gtccgttgac tctcagaacc aggaagttgt gttgggtcag gtacaaaaat ctgtatttgc
      361 tgcggttggt gacaaatccc cggcaaaacc tttcgaaatc aaactggaag attgcgatac
      421 caccacgatg aaaaaagcca atgtgagctt tagcggtgtt ggtgatgcag atcaatctga
      481 tctgatttct gttagtactg aagccggtgc ggcgaaaggt gttggtatcg gtatttatga
      541 taacagcaac accctggtcg ctcttaacgg tggtaaagca agtgttgatc tgagtaaagg
      601 ccagacggtt ctctacttta ccgccaacta cgtttcaact ctggcaacgg taacgaccgg
      661 ttacggcaat gcgcaggtcg atttcaacct gtcttacgag taatcgtctg ttttacccat
      721 ggcggcgtaa ttgccgccat tagtcgtcaa aattcatgga tcgaatgatg aaaagtaaat
      781 ttgttgcgtt ggctctgtct ctgttcctct cccagtcagt tctggcgggg ggcgttgggc
      841 ttagtgctac ccgtgtgatt tatgatggca gtaagaaaga agcctcgctg accgtacaga
      901 ataaaagtaa gacggaagag tttctgatcc agtcctgggt ggatgatgcc gcgggcagca
      961 aaaaaacacc gtttataatt accccgccgt tgttcaggct cgatcctgga aaaaacaata
     1021 tattgcgtat tgtaaatact acccctggcc tgccgcagga tcgcgaatcg gtctactggg
     1081 ttaatgtgaa agccattccg tctaaaagtg acgacagcga aaacaaaaat gtattacaga
     1141 ttgctgttcg cacccgcatc aaactgtttt atcgcccggc agggttacag ggtgatgtaa
     1201 aaaccgcgcc aaacgaactg cgatttacgc gtagcggtaa tcagatccgt gtcgataacc
     1261 ccaccgtttt taatattacc tttaatcaat tttttgcgaa tgacaaagaa attgaaaaag
     1321 ccggcatggt acccgcaaaa gggtcattaa atattacatt acccgccggg gcaggtagcg
     1381 tttcgaagat taaatataac actatcaatg actttggatc gtcagcggaa atgatgacta
     1441 aaaatgttga ttgattaata ccttttcagg agaaattgct caatgtcacg gaagaccgtt
     1501 tcacgtactt tttcttcctt ttctatctct gtggtggcgg ttgctgttgc cagcacgttt
     1561 tctgcacatg ccggaaagtt taaccccaaa ttcctggagg acgttcaggg tgtgggccag
     1621 catgtcgatt taacgatgtt cgaaaaaggc caggaacagc agttacccgg catctatcgt
     1681 gtctctgttt acgtcaatga acagagaatg gaaacccgca cactggaatt taaagaagcc
     1741 acagaagcac agcgcaaagc gatgggggaa tcccttgtgc cttgccttag ccggacgcaa
     1801 ctggcagaga tgggcgtgcg cgttgaaagc tttccggccc tcaatctggt gcctgccgaa
     1861 gcctgcgtgc cgtttgacga aatcattccc caggcctcaa gccatttcga cttcagtgaa
     1921 caaaagctgg tattaagttt ccctcaggcg gcgatgcacc aggtggcgcg tggcacggtg
     1981 ccggaatctc tatgggacga aggtatccct gcgttgctgc tggattacag ttttagcggg
     2041 agcaacagtg aatatgactc gacggggtct tccagcagtt atgtcgatga taatggcact
     2101 gttcatcatg atgatggtaa agatacgcta aagagcgaca gctactacct caacttacgc
     2161 agcgggctga accttggcgc ttggcggtta cggaattaca gtacctggag ccattccggt
     2221 ggcaaagcgc agtgggataa tatcggtacg tctttgagta gggccatcat ccctttcaaa
     2281 gcgcaattaa caatggggga tacggcgacc gcgggggata tttttgacag cgttcagatg
     2341 cgtggcgcga tgttagcctc tgatgaagag atgctgcccg acagccagcg aggctttgca
     2401 cccatcgttc gagggatcgc caaaagcaac gccgaagtca gtattgaaca aaacggctac
     2461 gtgatttatc gcacctatgt gcagcccggt gcctttgaaa taaacgatct ttaccctacc
     2521 gcaaacagcg gtgacctcac ggtcatcatt aaagaagcgg atggcagcga acaacgcttt
     2581 attcagccat tctcatcggt gccaattttc cagcgtgaag gccatctcaa atatagcttt
     2641 gccgcgggtg aatatcaggc cgggaattat gacagcggct cgccgcgttt cgggcagctt
     2701 gatctgatct acggtttacc gtgggggatg acggcctacg gcggcgtatt aatctctaat
     2761 aattacaatg catttgcatt agggataggg aaaaactttg gttatatcgg ggcgatttcc
     2821 attgatgtga cgcaggctaa aagcgaactg aataacgatc gcgatagcca gggacaatct
     2881 tatcgtttct tatattccaa gagcttcgaa agcggcaccg atttccgcct tgcgggctat
     2941 cggtactcta ccagcggttt ctataccttc caggaagcca ccgatgtgcg cagtgacgct
     3001 gacagcgact ataaccgtta tcacaagcgc agcgaaatac agggtaacct gacgcagcaa
     3061 ttaggggcct atggctctgt ttatttaaat ttaacgcagc aggattactg gaacgacgca
     3121 ggtaaacaga acacggtatc ggcgggttac aacggacgta ttggcaaggt cagttacagt
     3181 attgcatata gctggaataa aagccctgaa tgggatgaaa gcgatcgctt gtggtctttc
     3241 aatatttccg ttccactagg ccgggcctgg agtaactatc gcgtcacgac cgaccaggat
     3301 ggtcgtacca atcaacaggt tggggtcagc ggaacgctgc ttgaggatcg caacctgagc
     3361 tacagtgtcc aggaaggcta cgccagcaac ggtgtgggta acagcggtaa cgctaacgtt
     3421 ggctatcagg gtgggtccgg taatgtcaac gtaggctata gctacgggaa agattaccgg
     3481 cagctcaact acagcgttcg cggcggcgtg atagttcata gcgaaggcgt gacgctttcc
     3541 caaccgctag gcgaaaccat gacgctcatc tccgtacccg gtgcgcgcaa tgcccgcgtg
     3601 gtgaataacg gcggcgttca ggttgactgg atgggtaacg cgatcgtgcc ttacgccatg
     3661 ccgtatcgtg aaaacgaaat ctcactgcgt agcgattcgt tgggtgacga tgttgacgtt
     3721 gaaaatgcgt tccagaaagt ggtgccaacg cgtggagcga ttgtcagagc gcgttttgat
     3781 acccgcgttg gttaccgcgt attaatgacg ctgcttcgtt ccgcgggcag cccggtgccc
     3841 tttggagcaa cggcaacgct aatcaccgat aaacaaaacg aggtgagcag tatcgtcggt
     3901 gaggaagggc agctctatat tagcgggatg ccagaggaag ggcgggtact gattaaatgg
     3961 ggtaatgacg cgtcgcagca atgcgtggct ccatataaat tatccctgga attaaaacag
     4021 ggcggaattg ttcctgtttc agccaattgc cagtaagcaa ggagagcgaa atgaaagcgg
     4081 caatagcatt atctttatta ggatgcgtat tcggattttc tggcaaggca ttggccggag
     4141 gttcctgggg gccatgtacc cctgtggatg gcacgaccta tcactataac gtcgatgtgg
     4201 atgtcggtgt tcccgatgca agtaaaaacg tcgcagggac ggttttaacg gatgtcctta
     4261 actggtcgaa tggacaaaat gtatcgataa tttgtgaatg tcctgataag ggtgactata
     4321 aaattgaaaa agatacgttg atacaagggg ttagtggaat agcgaatatg accagggata
     4381 tcggtggttt taagtattat tacttaaccg atgaactgtc tgtatccaca aagattcagg
     4441 taagttcggg gcaatatggt tttgtcccct ttaaaggtca acaggcgctt caggtaacgg
     4501 gctgtaatga agtcatcact actccctata tgggcggagg aggattactt tctttcttga
     4561 ttacaaaacc ctttatcggc gattcagtca tccctcccac gttaattgcc gaactgtatg
     4621 cctccaaaac aaataaagat tacggaacaa tgccaatatc ttccgtatct attcagggcc
     4681 gcgtcaccat aacccaggat tgcgaaatta aacctggcac ggtgctggat gtgccatttg
     4741 gtgaattccc ctcctcagcg tttaaaaaca gacaagggca aatgcctgaa ggtgcgacag
     4801 agcaagaaat taacctcggc tttgattgca acaatatctc ggatggtatc aaagtctcgt
     4861 tgcgccttga gggggcaacc aatgctgacg atccccgagc ggtggatatg ggcaaccccg
     4921 atattggcgt actggtggaa gattccagcg gcaaaatttt ggtaccgaat gatgcaagca
     4981 gcaccacgtt attaaatttg agcagcctgg attctaaaac tcaccgcaat gccgcgattc
     5041 gactgctggc tttaccgatt agcacgacgg gtaaagcgcc taaaggcggg acgtttgaag
     5101 gcgtaacgac catatatctt gaaatggagt gatgccatga aattcaatct attgctgcat
     5161 agcggcatcg ccagtttgag tctgtttgcc tgcggggtga atgcggcgac ggatcttggc
     5221 ccggcagggg atattcattt ctccatcact atcaccacta aagcttgcga gatggaaaaa
     5281 agcgatctcg aagtcgatat gggaacaatg acgctgcaaa aacctgcggc agtcggtacg
     5341 gtgttgagca agaaagattt tactattgaa ctcaaagagt gcgatgggat atccaaagcg
     5401 accgttgaga tggacagtca gtcggacagc gatgatgatt ccatgtttgc ccttgaggct
     5461 ggtggcgcaa cgggtgttgc gttgaagata gaggacgata aaggaacgca gcaagttccc
     5521 aaaggctcca gcggaacgcc gattgaatgg gcgattgatg gcgaaaccac gtcgcttcac
     5581 taccaggcga gttatgtggt cgtcaacact caggccactg gtggcacagc gaatgccctt
     5641 gtcaattttt ccatcaccta tgagtaagtt actgggccag catcctggcc caacctatct
     5701 ctgaatagtc gttaatccgt taaaatggtc tattccggac ttgcactttt gtaaatttgt
     5761 gcttcatgca cactctttcc ccacactttt tccctttgct gtggtctact tattcgcgcg
     5821 tgtagatttt acttatctga ctacctccgc acttttcccc tgctgggcct gaaaagccgc
     5881 taagcagggt gttatcacct gtttgtccag ggtttgtttg catgagatac atcaaatcga
     5941 ttacacagca gaagctgagc tttttgcttg caatctatat tggccttttt atgaatggcg
     6001 cggtttttta ccgccgcttc ggcagctatg cgcacgattt taccgtctgg aaaggcattt
     6061 ctgctgttgt tgaactggcc gccaccgtac tggtgacctt ctttttacta cgtcttcttt
     6121 cgctgtttgg ccgccgcagt tggcgtattc tggcatcgct ggttgtgctc ttttccgcag
     6181 gtgccagcta ttacatgacc ttccttaatg tggtcattgg ttatggcatc atcgcttccg
     6241 tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt attctctggt
     6301 taatcgccgt tagcgcattg cctcttatcc ttatctggaa taaccgctgt cgctacacct
     6361 tgctccgaca actgcgaacc ccagggcagc gtattcgcag cctggcggtc gtcgtactgg
     6421 cgggaattat ggtttgggca ccgattcgtt tgctggatat ccagcagaag aaagtggaga
     6481 gggcgaccgg cgttgatttg ccgagttatg gcggtgtcgt agcgaactct tatctgccat
     6541 caaactggct ttctgcgttg gggctgtacg cctgggcgcg ggtcgatgaa tcttccgata
     6601 ataattcatt gcttaacccg gcgaagaaat tcacttatca ggcaccgcaa aatgttaatg
     6661 acacttatgt cgtgtttatc atcggtgaaa ccacgcgttg ggaccatatg ggcattttcg
     6721 gctatgagcg taataccacg ccgaagctgg cccaggagaa aaatctggcg gcgttccgtg
     6781 gttactcctg tgataccgca accaaactct ctctgcgttg catgtttgta cgtcaggggg
     6841 gcgcggaaga taatccgcag cgcacattaa aagaacagaa cattttcgcg gtactgaagc
     6901 agttaggatt cagttctgac ctctacgcta tgcagagcga aatgtggttc tacagcaata
     6961 cgatggcgga caatattgct taccgtgaac agattggtgc ggagccacgt aaccgtggca
     7021 agccggtaga tgatatgttg ctggtagacg aaatgcagca atcgctgggg cgcaacccgg
     7081 atggtaagca tctaatcatt ctgcatacca aaggttcgca ctttaactac acccagcgtt
     7141 acccgcgcag ctttgcgcag tggaagccgg aatgtattgg tgttgatagc ggatgtacca
     7201 aagcgcagat gatcaactcc tatgacaact cggtgaccta tgtggatcac tttatctcca
     7261 gtgtgattga tcaggttcgc gataagaaag ctattgtgtt ctacgccgct gaccacggcg
     7321 agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg tgaactggca ccggcggagc
     7381 agttccgcgt accgatgatg gtctggatgt cagataaata tctggaaaat ccggtcaatg
     7441 cgcaggcgtt tgcgcagctg aaaaaagcag ccgacatgaa agtgccacgc cgtcacgtag
     7501 agctgtacga caccatcatg ggttgtcttg gctatacttc accggatggt ggaattaacg
     7561 aaaacaacaa ctggtgtcac atcccgcaga caaaagaggc agtggctaac taagaggcct
     7621 gctgactttc tcgccgatca aaaggcattt tgctattaag ggattgacga gggcgtatct
     7681 gcgcagtaag atgcgccccg cattcggtga ttggcgcagc ctggtagcgc acttcgttcg
     7741 ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc aatttagaaa aaccagccta
     7801 cgggctggtt ttttgctttc tgcgacctga agaggatgag aacctccggg ggcagggagg
     7861 ttcgactcga cgtaggcctg ataagacgcg tcagcgtcgc atcaggcaac ggcaccgcac
     7921 tcattgtcgg atgcagcgta cccgtcttat ccgacctaaa cggcagcaca ccacacattt
     7981 ccctgccaaa tttgcgcccc atatctcact atccattaac ggatttgtga cagaaaccat
     8041 tgtctttttt tatatttgct taaatctttt tttgcgttac ttatggctgg cgctggcatt
     8101 ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc ttttcctcag
     8161 atttttttca ctaaatgcat aaaacttaat caccaaatgt tgctaaatat taagctgatt
     8221 gttctggttg tgttggagta gcacaaattt ctctgctgca aatagctgtt tgaagttttt
     8281 ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg tattgacgtt
     8341 ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga tccgcactgt
     8401 tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga tcgggcaaaa
     8461 aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca aacatcacaa
     8521 ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc ttggtctcag
     8581 cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt attgctcaga
     8641 aggatctccg gaagggttta acccgcagct gtttacctcc ggtaccacct atgacgcctc
     8701 ttccgtaccg ctttataacc gcctggttga atttaaaatc ggcaccaccg aagtgatccc
     8761 gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct tccatctgcg
     8821 taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa tgaatgccga
     8881 tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc ataaagtttc
     8941 tggcggtagc tacgaatact tcgaaggcat gggcttgccg gagctgatca gcgaagtgaa
     9001 aaaggtggac gacaacaccg ttcagtttgt gctgactcgc ccggaagcgc cgttcctcgc
     9061 tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgatg cgatgatgaa
     9121 agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt tccagttaca
     9181 gcagtatcaa aaagattccc gtatccgcta caaagcgttt gatggctact ggggcaccaa
     9241 accgcagatc gatacgctgg ttttctctat tacccctgac gcttccgtgc gttacgctaa
     9301 attgcagaaa aatgaatgcc aggtgatgcc gtacccgaac ccggcagata tcgcccgcat
     9361 gaagcaggat aaatctatca acctgatgga aatgccgggg ctgaacgtcg gttatctctc
     9421 gtataacgtg cagaaaaaac cactcgatga tgtgaaagtt cgccaggctc tgacctacgc
     9481 ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat cagcgaaaaa
     9541 cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggactaca cctacgatcc
     9601 agaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct ccatcgacct
     9661 gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg cggagatgat
     9721 tcaggcagac tgggcgaaag tcggcgtgca ggccaaaatt gtcacctacg aatggggtga
     9781 gtacctcaag cgtgcgaaag atggcgagca ccagacggta atgatgggct ggactggcga
     9841 taacggggat ccggataact tcttcgccac cctgttcagc tgcgccgcct ctgaacaagg
     9901 ctccaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac cggcgcgtgc
     9961 taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg tgatgcacga
    10021 tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac gcaaagaagt
    10081 taaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct ctatcgaata
    10141 attaaaagcc atacccggtg gtgctgtgcc tctgcgtgca gcgccatccg gcagcaatac
    10201 ttcattccct gcccgacctg ggcgggaatt gatttgtgag caatacagac acgcagttcc
    10261 aggctgcggg tcactacaga gaatccgggt tatgttgcag tttattctcc gacgtttggg
    10321 actcgtcatc cccacgttta tcggtattac ccttctcaca tttgcctttg tccacatgat
    10381 cccgggcgat ccggtgatga tcatggcggg cgaacgtggg atctccccag agcgtcacgc
    10441 gcagttgctg gctgaactcg gcttagataa accgatgtgg cagcagtatc tccattacat
    10501 ttggggcgtt atgcatggcg atctaggcat ttcaatgaaa agccgcatcc cggtatggga
    10561 agagttcgtg ccgcgctttc aggccacgct ggaacttggc gtctgcgcga tgatttttgc
    10621 tacggcagtc ggtattccgg ttggtgtgct ggctgcggtt aaacgtggtt ccattttcga
    10681 tcacacagcg gttggcctgg cgctgacagg ttattcaatg cctatcttct ggtggggcat
    10741 gatgctgatc atgctggttt cggtgcactg gaacctgaca cccgtctccg gtcgcgtgag
    10801 cgatatggtg ttcctcgatg actccaatcc gttaaccggt tttatgctga tcgacaccgc
    10861 catctggggt gaagacggta actttatcga tgccgtcgcc catatgatct tgcccgccat
    10921 tgtgctgggt actattccgc tggcggtcat tgtgcgtatg acacgctcct cgatgctgga
    10981 agtgctgggc gaggattaca tccgcaccgc gcgcgccaaa gggttgaccc gccttagggt
    11041 gattatcgtc catgcgctgc gtaacgcgat gctgccggta gtgaccgtta tcggcctcca
    11101 ggtgggaaca ttgctggcgg gggcgattct gaccgaaacc atcttctcgt ggcccggtct
    11161 ggggcgctgg ttgattgacg cactgcaacg ccgcgattat ccggtagtgc agggcggcgt
    11221 attgctggtg gcgacgatga ttatcctcgt caacttgctg gtcgatctgc tgtacggcgt
    11281 ggtgaacccg cgtattcgtc ataagaagta aggggacatc atgtcacagg ttactgaaaa
    11341 taaagtgatt agcgcaccgg tgccgatgac cccattacag gagttctggc actattttaa
    11401 acgcaacaaa ggcgcggtcg tcgggctggt ttacgtcgtc atcgtgctgt ttatcgcgat
    11461 ctttgccaac tggattgcac cctataaccc ggcggaacag ttccgcgatg cactgctcgc
    11521 cccgccagcc tggcaggaag gcggcagcat ggcgcacttg ctgggcaccg atgacgtagg
    11581 ccgtgatgtg ctgtcgcggc tgatgtacgg cgcgcgttta tcgctgctgg ttggctgtct
    11641 ggtggtggtg ttatcgctga ttatgggcgt tattctcggc ctgatcgccg gttactttgg
    11701 cggcctggtc gataacatca tcatgcgcgt ggtcgatatc atgctggcgc tgccaagtct
    11761 gctgttggca ctggtgctgg tggcgatttt cggcccgtcg attggtaacg ccgcgctggc
    11821 gctgaccttt gttgccttgc cgcactatgt gcgcttaacc cgcgccgccg tgctggtgga
    11881 agttaaccgc gattacgtta ccgcgtctcg cgtggcaggt gccggggcga tgcgtcagat
    11941 gtttattaac atcttcccga actgccttgc gcctctgatt gttcaggcgt cgctcggttt
    12001 ctctaacgcc attctcgata tggctgctct tggtttcctc ggcatggggg cgcagccgcc
    12061 aacgccggag tggggcacca tgctctccga cgtgttgcag ttcgcgcaaa gcgcctggtg
    12121 ggtcgtgacc ttcccgggtc tggcgatcct gctgacggtg ctggcattta acctgatggg
    12181 tgacggtctg cgtgacgcgc tcgatcccaa actgaagcag taagaggttc gagatggcgt
    12241 tattaaatgt agataaatta tcggtgcatt tcggcgacga aagcgcaccg ttccgcgccg
    12301 tagaccgcat cagctacagc gtgaaacagg gcgaagtggt cgggattgtg ggtgagtccg
    12361 gctctggtaa gtcggtcagt tcactggcga ttatggggct gattgattat ccgggccgcg
    12421 tgatggcgga aaagctggag tttaacggtc aggatttgca gcgtatctca gaaaaagagc
    12481 gccgcaatct ggtgggtgcc gaagtggcga tgatcttcca ggacccgatg accagcctta
    12541 acccgtgcta caccgttggt ttccagatta tggaagcgat taaggtgcat cagggcggta
    12601 ataagagcac tcgccgtcag cgggcgattg acctgctgaa tcaggtcggt attcccgatc
    12661 cggcatcgcg tctggatgtt tacccgcatc agctttccgg cggcatgagc cagcgcgtga
    12721 tgatcgccat ggcgattgcc tgtcggccaa aactgctgat tgccgatgaa ccgaccactg
    12781 cgctggacgt gaccattcag gcgcaaatca tcgaactact gctggagcta cagcagaaag
    12841 agaacatggc gctggtgtta attacccatg acctggcgct ggtggcggaa gcggcacata
    12901 aaatcatcgt gatgtatgcc ggtcaggtgg tggaaacggg cgatgcgcac gccatcttcc
    12961 atgcgccgcg tcacccgtat actcaggcat tgctgcgcgc gctgccggaa tttgctcagg
    13021 acaaagaacg tctggcatcg ttgcctggcg ttgttcccgg caagtacgac cgcccgaacg
    13081 gctgcctgct taacccgcgc tgcccctatg ccactgacag atgtcgcgct gaagaaccgg
    13141 cgctgaatat gctcgctgac gggcgtcagt ccaaatgcca ttacccactt gatgatgccg
    13201 ggaggccgac actatgagta cgcaagaggc cacctcgcaa caaccgctgt tgcaggctat
    13261 cgacctgaaa aaacattatc cggtgaagaa aggtatgttc gctccggaac gtctggtgaa
    13321 ggcgctggac ggcgtttcgt ttaaccttga acgtggcaaa acgctggcgg tagtaggtga
    13381 atctggctgc ggtaaatcga ccctcggtcg gttgctgacg atgattgaaa cgcccaccgg
    13441 cggcgagctg tattaccagg ggcaggatct gcttaagcac gatccgcagg cgcagaagct
    13501 gcgtcggcag aaaatccaga ttgtcttcca gaacccttac ggttcgctga atccgcgtaa
    13561 aaaagtcggg caaattcttg aagagccgct gctgatcaac accagcttaa gcaaagaaca
    13621 gcgtcgggaa aaagccctgt cgatgatggc gaaagtcggc ctgaaaaccg agcactatga
    13681 ccgctatccg catatgttct ccggcggaca acgtcagcgt atcgctattg cccgtggtct
    13741 gatgctcgac ccggacgtgg tgattgccga tgaaccggtc tccgcgctgg acgtctcggt
    13801 gcgtgcacag gtgctcaacc tgatgatgga tttgcagcag gaactggggc tttcttatgt
    13861 cttcatctcc cacgacctgt cggtggtgga gcacattgct gatgaagtga tggtgatgta
    13921 cctgggccgc tgcgtggaga agggaacgaa agaccaaatc ttcaataacc cgcgccatcc
    13981 gtacactcag gcgctacttt ccgcgacgcc gcgcctgaat ccggacgatc gccgcgagcg
    14041 catcaaactc accggtgaac tgccaagccc gctgaatcca ccgccgggct gcgcgtttaa
    14101 cgcccgctgt cgtcggcgct tcggcccctg cacccagttg cagccgcagc taaaagacta
    14161 cggcggtcaa ctggtagctt gttttgctgt tgatcaggat gaaaatccgc agcgttaact
    14221 cacaaaaccg gggccatgcg ctccggttta ctcaatcagc ttcaacagtg gcgacaagca
    14281 tagcaaaatg ccgtaaagca gaatcagcca ggctttaaat ccgcgaagtt tttccagttg
    14341 tgcgacttta tagatcagga aaaacggaat gaggcacgac acaatgccat ataacgggct
    14401 tcccaactga aagaacacca gcaccgatac acgaaacgaa acccaaatcg tcaacgtaat
    14461 gacgataaaa gcgcagatcg ccagagtcag cacgcgtgag ttaattttct tggtatcaat
    14521 aattcggctt aacagattga gaataatgcc tttaatggcc tcgtggaaac cgagataaat
    14581 gccaaagaat gcggtcagta cggcaaagat attcagcacc gtagaggtga tatgaatgat
    14641 atgcccaggg atcacctgcg cggccagtgc cagtgccgag atattttgtt caaaggcaga
    14701 aacggcttct tcgtggctaa ttgagaaggt aaacgaaaag gcaaaaaaca gtatcaccgc
    14761 gatgagcgtg atataactaa ttcggtgggt acgcagcgcg agccgggttg ccagtacttt
    14821 atccgcttcc cgtttacgat aggcaatatt cattgggtta agtacctgaa taaatactgc
    14881 agaaaagaag caaaatggaa tggtaagcag gacatcgcgg aaaaagacgg aggcttgcgg
    14941 gaaggcggtt atattggcga aattccagtg cgggatcatc gcaaaaccga acacgacaat
    15001 aatccctact ttgaccacca ccattggccc agaaatctta aacagtaatc gttcaccacc
    15061 agacgcaatc gctaccagta cggcgaaaat agcgacttta taaagtagag attgtgaaag
    15121 atcggcatcg gttaaaccga aggttttcag gtacgaggcg ctgtcgaaaa ccacggagag
    15181 agagtagata aaaatcccgt ggataatcat caaaaagtag ataaccccga ggaaaattcc
    15241 ccagttcttc cccaggtaat gactgataat atcggtgtag tcattacagg aatcgctttc
    15301 agaaagggtt tttaaataaa tgtcctgcac tacccaggtg gcaggataag caatgatcgc
    15361 tgcggtaata aatacccaaa ttcccttcaa gccaatttgt actggcatca gcacggttcc
    15421 ggcaccaatc gccatgccta tgcataataa aacccagccg aagtcgtagc gtgtaaacgg
    15481 caatttctga tcgtgtttcg gtagtgtgtt gtgctgcata aagtcctgtc cagaaagaag
    15541 tcggcctcgc aaaaatgaat aaacccagtt tctcttgcgc aacaggtgga gaagtcgaat
    15601 acgcagtatg taaaaaaatg aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat
    15661 tctacaaaat tgcgcatttc actaccagtc tggagtcagc ccggatatga actgcaacta
    15721 ttgcaaatta agaaattaag ctggaaggca tcacgcaatg aggaagattg ccgagtgctg
    15781 acagtaaaaa gcaatcgtaa aattagcggc aagccggaag ggttccggtg aggcgcaatg
    15841 ttgcgggggc ttaatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg
    15901 caggtatgca cctgacaaca ccacgggggc taatcttgaa cctagac
//
LOCUS       DAPGRN010000108        15887 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPGRN010000108 DAPGRN010000000
VERSION     DAPGRN010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.109"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..1734
                     /locus_tag="RHW33_003664"
     CDS             52..1734
                     /locus_tag="RHW33_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV1647127.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1786..2943
                     /locus_tag="RHW33_003665"
     CDS             1786..2943
                     /locus_tag="RHW33_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV1647128.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNDETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2909..3031)
                     /locus_tag="RHW33_003666"
     CDS             complement(2909..3031)
                     /locus_tag="RHW33_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647129.1"
                     /translation="MTKHPTGIYVGCLVKAIRRRLRLEQRGSVINYSPFVLQHP"
     gene            3234..4919
                     /locus_tag="RHW33_003667"
     CDS             3234..4919
                     /locus_tag="RHW33_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV1647130.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            4957..5298
                     /locus_tag="RHW33_003668"
                     /pseudo
     CDS             4957..5298
                     /locus_tag="RHW33_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
     gene            complement(5334..8300)
                     /locus_tag="RHW33_003669"
     CDS             complement(5334..8300)
                     /locus_tag="RHW33_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HDV1647131.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRAAPAAQGVLAAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPE
                     GLDRKFYEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAI
                     NPNSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALI
                     DQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLT
                     KMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQ
                     RFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRP
                     LIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSR
                     RLEDGKFRPLRMPGKP"
     gene            complement(8304..8864)
                     /locus_tag="RHW33_003670"
     CDS             complement(8304..8864)
                     /locus_tag="RHW33_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1647132.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            9174..10751
                     /locus_tag="RHW33_003671"
     CDS             9174..10751
                     /locus_tag="RHW33_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HDV1647133.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            10941..12974
                     /locus_tag="RHW33_003672"
                     /pseudo
     CDS             10941..12974
                     /locus_tag="RHW33_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            13031..15814
                     /locus_tag="RHW33_003673"
     CDS             13031..15814
                     /locus_tag="RHW33_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV1647134.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAETRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTIDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAQDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMAIDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         4155 a         3877 c         3972 g         3883 t
ORIGIN      
        1 tctcttcgta aaattacttt ggaattaagt acaataagaa gaggaacatt tatgaagtct
       61 gcattaaaga aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct
      121 gcatttgctg ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct
      181 ttctcagact ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtactg
      241 accatggatg atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa
      301 acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa
      361 gctgcgcaaa aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact
      421 aacggctatg tggcgcacgg tgtttccggc ccctcccgcg ccgcaataat gaccggtcga
      481 gcgcccgccc gctttggtgt ctattccaat actgatgctc aggatggtat tccgctaaca
      541 gaaactttct tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa
      601 tggcacttgt caaaaatcag taatgtgccg gtaccggaag ataaacaaac acgtgactat
      661 catgacaact tcaccacatt ttctgcggaa gaatggcaac cgcaaaaccg tggctttgat
      721 tactttatgg gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa
      781 aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt
      841 ggcgttgttg atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat
      901 gctccgcacc tgccaaatga taatcctgca ccggagcaat atcagaagca atttaatacc
      961 ggtagtcaaa cagcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa
     1021 cgcattctcg aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc
     1081 tccgataatg gtgcggttat cgatggccct ctgccgctga acggggcgca aaaaggctat
     1141 aagagtcaga cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa
     1201 cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc gacagctctt
     1261 gatgcagccg atatcagcat tccaaaagac cttaagctgg atggcgtttc cttgctgccc
     1321 tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat aacctcttat
     1381 tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa atttgtccgt
     1441 catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca attctcttat
     1501 acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc
     1561 tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt
     1621 aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag
     1681 gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac
     1741 cttcatgcgg cggaattttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc
     1801 aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag
     1861 tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa
     1921 tatatcgcag cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg
     1981 gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa
     2041 cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt
     2101 ctcaaagaac atgaatttct ggttggtatc tcgatcgatg gaccccagga gctacatgac
     2161 cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag
     2221 cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc
     2281 cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt
     2341 atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca
     2401 ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt
     2461 tttatgcaat gggttaaaaa cgatgtgggt gaaattttca tccgtcagtt tgaaagcttt
     2521 gtcagccgtt ttttggggaa tgggcatacc agttgtattt tccaggagtc ctgcaaggat
     2581 aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag
     2641 tacaaaattg gaaacattaa taaatctgaa cttaaaacga tgaacagtgt acaactgaca
     2701 gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg catataaacc tatctgcaat
     2761 ggcggttgcc ctaagcatcg tattactaaa gtaaacgatg agactgtttc ctatttttgc
     2821 gaaggttata aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggtggagtta
     2881 gccaagaata gagtaccgct ttaccacatt atggatgttg caaaacaaat ggagaataat
     2941 taataacgct ccctctttgc tccagtctta gcctccttct tatggcctta acaagacacc
     3001 cgacataaat tcctgtaggg tgtttcgtca tcatcatctt cccttgcgaa aattctggct
     3061 tacgtggtat gcgtcagggc attagcaata cccttgatta tcaatagcat aaccataacc
     3121 actgaaaatt aaaaatttct ctatatttca tattgttaaa tgatacttaa ataaatttca
     3181 tcagtaaatc attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa
     3241 ccattcccat tacgttttgt atgttgatag caaagtatct ctgtctgctg aagccatttt
     3301 ggctgcgtaa gaataataaa acttcagtgt tgttaatcat tattatcctc gccatgatcc
     3361 ttggcgtggt taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc
     3421 taagccagaa agaaaccgat aagctttggc agcttgtcct ttggttccct gccctgctgg
     3481 ggatctttgt attaatatca gtaaacaaaa catggttaat taaactgctg actattcgct
     3541 ggcgcgaatg gttaacagat tactacctta atcggtggtt tgcagataaa aactactact
     3601 tcacgcagat ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag
     3661 acattctctt actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta
     3721 gcatgctgat cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg
     3781 ttggtggaac agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga
     3841 tcggtggaac tttatttacc cataaggttg gtaaacgtat tcgtccgctt aatgtggaaa
     3901 aacaacgtag cgaagcgaca tttcgaacta atcttgtgca gcataataag caggctgaac
     3961 tgattgcgct aagcaatgct gagtctctac aacgccagga gttgagcgac aattttcata
     4021 ccattaaaga gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa
     4081 atatttattc gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta
     4141 gcgggcagat caatctgggc ggactgatga aatcgcgcca ggcatttatg ctggtatcga
     4201 acaatttaag ctggtttatc tataaatatg acgaacttgc tgaactggct gcggttatcg
     4261 atcgcttgta tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt
     4321 gccaacatgc ggtacaagtg gctgatgcga gtattcgtac gcctgataat aagatcatat
     4381 tagagaacct gaactttcat gtttcgccag gcaaatggct attactgaaa ggttactctg
     4441 gtgcgggaaa aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg
     4501 atatttcttc tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct
     4561 tactgaaaga gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg
     4621 aagtactgca tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct
     4681 ggggagatat tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac
     4741 gacgtccgaa atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta
     4801 tccgcttact gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac
     4861 atcaacccgg tgtctggaac ctggcagatg atatttgtga cattagcgcg gttttataaa
     4921 aacaataaaa gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc
     4981 gtcattttat gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat
     5041 gtttttgaag agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat
     5101 tacaccagtg aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg
     5161 agaaccaacc ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat
     5221 attcgcccgg agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt
     5281 gatggtatta gtgccactaa ggggagcccg cagaattcgg aaaaaatcgt acgctaaggt
     5341 tttccgggca tccgtaaggg ccgaaacttc ccgtcttcca gtctgcggct ctgccgccag
     5401 acgtaatcgc cggttaggtt gatgtgctcc cagcccagcg gcgacaggaa ttgcagcagc
     5461 tcgccgtcca ccggcttgcc ggcctcgacc aacccctggg tggcgcgttc caggtacacc
     5521 gtgttccaca gcacgatagc cgccgtcacc aggttgaggc cgctggcccg gtagcgctgc
     5581 tgctcgaagc tccgatccct gatttcccca aggcggttga agaacaccgc cctggccagc
     5641 gagttgcgcg cctcaccttt gttcaggccg gcatgcacgc ggcggcgcag ttcaacactt
     5701 tgcagccagt ccaggatgaa cagcgtgcgc tcgatccggc ccagctcgcg cagggccacg
     5761 gccagtccgt tctggcgcgg gtagctgccg agcttgcgca gcatcagcga ggcggtgacg
     5821 gtgccctgct tgatcgagct ggccaggcgc aggatgtcgt cccagtgggc acgcacgtgc
     5881 ttgatgttca gggtgccgcc gatcagcggg cgcaacgtcg ggtaggcttg cacgccctgc
     5941 ggcacgtaca gcttggtttc gccgaggtcg cggatgcgcg gcgcgaagcg gaagcctagc
     6001 aggtgcatca gggcaaagac gtgatcggtg aagccggccg tgtcggtgta gtgctcctcg
     6061 atccgcaggt cggactcgtg gtacagcagg ccgtcgagca cataggtgga atcgcggacg
     6121 ccgacattca ccacgcgggt gctgaacggc gcgtactggt cggagatatg ggtatagaac
     6181 agccgtcccg gctcgctacc gtacttcggg ttgacgtgcc cggtgctctc gccccggcca
     6241 cccgcgcgga agcgctggcc atcggaggat gaggtcgtgc cgtcgcccca gtgggcggca
     6301 aaggcgtggc gatactggtg gttgaccagc tcggccaagg ccgccgaata ggtttcgtcg
     6361 cggatgtgcc aggcttgcag ccaggacagc ttggcgtagg tcaggccggg gctcgactcg
     6421 gccatcttgg tcagcccgag gttgatcgca tcaccgagga ttgcggacag cagcaacgtc
     6481 ctgtctttgg cctcggcccc gtccttcaag tgggtgaagt ggcggctgaa gcccgtccag
     6541 tcgtccacgt ccatcagcag ttcggtgatc ttgatgcgcg gcagtaactg gctggtttgg
     6601 tcgatcagcg cctgcgcccg atccggcacc gccgcatcca gcggggtgat tttcagccct
     6661 gactcggtga ggatggcatc gggcagctcg ttgtccttgg ccaggcgggt gacggtggcc
     6721 aactgctcgt ccagcagctg caaacgctct tccaggtact ggtcgctgtt cgggttgatc
     6781 gccaggggca gggcctgctc gcgcttgagt gcggcgaact tctcggccgg cagcaggtag
     6841 tcgtcgaagt cgcggaactg ccgcgagccc ttgacccaga tgtcgccgga gcgcagggcg
     6901 ttcttcagct cggacagggc gcagatttcg tagaatttcc ggtcgaggcc ttccggggtg
     6961 atcaccagcg gcttccagcg cggcttgatg aaggccgtgg gtgcatcggc cggcaccttg
     7021 cgcaggttgt cggcgttcat ctcacgcagg gtctgcacgg ctgccagcac gccttgcgcg
     7081 gccggcgcgg cgcgcagttc cagcacctcc agcaaggccg gcgtgtaacg gcgcagggtg
     7141 gcgaagttct cgccgaccag gtgcaggtgg tcgaagcctt ccggccgggc cagcagctcg
     7201 gcctcgctga cgctctcggt gaactcgtcc cagggaatca ccgcctcgat ggcggcatag
     7261 gggtcgctgc cgctttcctt cgcttccagc agcgcctggc cgatcctgga gtacaggcgc
     7321 accttgtcgt tgatcgcctt gccctgcttc tggaactgct gctgatgctt gtgcttcgcg
     7381 ccgctgaaca gcttgaccag gatgcggtca tgcagatcga ccagctcatc gatcacggtc
     7441 gcggtgctct ccagcaccac ggcggccagg gtcgcgtagc ggcgctgcgg ctcgaactta
     7501 ccgaggtctt tgggcgtcat ctgcccaccc tcgcgggcca gcttgagcag gcggttctgg
     7561 tggatgtgcc ggcccaggcc ttcgggcaag tccaccaact gaaatgtctt cagccgctcg
     7621 atgtgttcca gcatgtgccg agagttgggt ttcagcggtg cctggcgcag ccaggtcaac
     7681 caggtgatgc tgctgccggc cttgagcttc aacagctcgt ccagcttggc ccgatgcgag
     7741 tccgtgagtg gttcgaccag ggcgcggtag acccgccgat tggctcgcgc aatggcttcc
     7801 gagcaggccc ggtcaatcac gctcagcgcc ggcaggatgc gtcgtttctg ccgtaggctc
     7861 tccagggcct gaccggccag cagcaagcct ttgtcggtct gctgggccag ctcggttagc
     7921 tcgcgcacca gggcgcggaa gtcggacagg ccgaacgggg ccagttgcag gtaggtgcgc
     7981 agttcctggg catgctcgcg acgggtcacg tcgcgctcgc cgtactttgc ccagctcgcc
     8041 ggctcggcct ggacttgctt cgccacccac aggatgaccg gctcgggcag ctcgctgtcg
     8101 gttcccagcg cgtaaccggg gtagcgcagc aggcagagct gcacggcgaa gccgaggcgg
     8161 ttggcgtcgc cgcgtcgctg gcggatcagc gacaggtcgg agtcgttgaa ggtgtagtag
     8221 cggatcaggt catcctggct ttccggcagc gcaagcaggg tgtcccgctc cgtggccgag
     8281 aggatcaagc gacgcggcat gtgtcagtcg tccgtgcgga ggtactggta gagggtttcc
     8341 cggctgatgt tgaactcgcg ggcaagctgc gccttgggct cgccggccgt cgctcgctgc
     8401 cgcagggtag cagcctgctc atcggacagg gctttcttgc ggccccggta cgcgccacgc
     8461 tgcttggcca aggcgatgcc ctcacgctgc cgctcgcgga tcagggcgcg ctcgaactca
     8521 gcgaaggccc ccatcaccga cagcatcagg ttggccatcg gcgagtcctc gccagtgaac
     8581 accaggccct ccttcaggaa ctcgatgcgc acgccgcgct gagtcagctt ctgtaccaag
     8641 cgacgcaggt catcgaggtt gcgggccagc cgatccatgc tgtgcaccac cactgtatcg
     8701 ccttcgcgga cgaagctcag cagcgcttcg agctgggggc gctgggtgtc cttgcccgat
     8761 gccttgtcgg tgaacacctt gctcacctgg gtttgttcca gctggcgttc cgggttctgg
     8821 tcaaagctgc tgacccggac gtagccgatg cggtgcccct gcacgatgtc tccttggttg
     8881 aaggcggctt aagtgcactt tctgttccgt tgtgcctcaa agcccatttc tgtcaggctg
     8941 aaatctataa ccttcgcggg catgtgtcaa aaaatgggaa agcagactct attctgacga
     9001 agcggcgcgg ccctgcctga catcaagtta gggtatagcc tagattgaca tgcgcgatgc
     9061 aacccttaac ttgcttgcac ctatcgtttc catgctagct ttatcgtaac gctcaagaaa
     9121 ttgggcttaa tgccgaaaac aataaaataa aacagccaac ccttgaggtt cttatgcgcc
     9181 agaatttacc agtgacgggt cgaaacttag aactcccaaa agatgccaat attctttcga
     9241 ctacctcccc tcaaagccat atcacgtacg ttaatcctga cttcattaaa atcagtggtt
     9301 tcactgagga agaactatta ggccagcctc acaacatcgt aagacaccca gatatgccgc
     9361 ctgctgcatt tgagcatatg tggagtacat taaaatctgg ccgctcatgg atggggctag
     9421 taaaaaatcg ctgtaaaaat ggcgaccact attgggtaag tgcttatgta acgccaatag
     9481 ctaagaatgg ttcgattgtt gaataccagt ctgtaaggac caagcctgaa cctgagcagg
     9541 ttttggctgc ggaaaaatta tatgctcaat tgagaagcgg gaaggccgcg aggccgaaat
     9601 tggctgctag cttttccgtg aaaatactct tgctcatatg gggtagtatt atatcaagcg
     9661 caatggctgc cggcatgctt actgatacat caataagcag cttattgtta gccactttaa
     9721 tgtcaggaag cttaagctct gttagtgttt tggctattct ctctcctctt ggaagactgg
     9781 ttgaaagagc caggaatatt tccaataacc cattaagtca atccctctac actgggcgca
     9841 ccgatgagtt tggccaaata gagtttgctt tacgaatgat gcaagctgaa acaggcgcca
     9901 tagtaggtcg cataggtgat gcatcaaatc ggcttagcga acacacccga ggcctactaa
     9961 aggatattga gtcaagcaat gtacttacag ttgagcagca ggcagagaca gatcaaatag
    10021 caacggcagt aaaccaaatg gtggcaagca ttcaagaggt tgcgagcaat gcacagcatg
    10081 ctgcagatgc ggccggaaga gcagacactg agacggcatc tggccagcgt ctggtagccc
    10141 acacaagcca gtcaatcact gcccttgaag gtgaaattag gcaagccact caggttattc
    10201 atgagcttga aggtcaaagt aacgagatat caaaagttct tgacgttata cgagggatcg
    10261 ccgagcaaac gaatttgttg gcactcaacg cagcaattga ggccgcgcgt gctggtgagc
    10321 aggggcgtgg ttttgctgtt gtcgccgatg aggttcgcag tcttgctgct cgcacacagc
    10381 aatcgacaac ggatattcaa agcatgatca gcgctctaca agagcgagcg caatccgctg
    10441 ttacagtcat ggagcaaagt agtcggcaag cgcacacgag tgtagctcac gcagaggaag
    10501 cagctacagc tcttgatgga attggccaac gcgttaacga aattaccgac atgaacgcgc
    10561 aaatagcgac tgcggtcgag cagcagggag cagtaagtga agacataaac cgcagtatta
    10621 tcaatatacg cgatgctgct gataccaatg tacagaccgg gcagaataat ttgcaaagtg
    10681 cgaaatctgt cgctcagtta actagcgctc tgagcgaact ggcaaaacag ttttgggaaa
    10741 aacgaggata acgcttttca gtatctcgac agggataact tactttacca tcggttatcc
    10801 cttttagccg cacaaatttt agccatggct tttcaggaag tcagggctat cagaatggcc
    10861 ttagaaagcc tagtcaaaga gctgtcacga gaacaccgtt agcttagcgt acgatttttt
    10921 ccgaattctg cggttccccc actaataacc tgaacccagc gaatgagtcc gatgccagta
    10981 gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca
    11041 atgtgacgct ttatgacagt tttgtgccgg ttgaattcgg tcgcttcaat ggcggggtaa
    11101 ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcag cgtgaaactg ggttatcgca
    11161 ctacgcgttc ggactggtta acatcgcata tcgatgagaa taacaagagc gcatttaatc
    11221 aaggttcttc aggaagtact tattactctc cagattttaa aaagaacttt tataccttgt
    11281 cgtttaatca ggaactcgct gataactttg gcgttaccgc cggtttatcg cgccgccagt
    11341 ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcgccggt cgggcacagt
    11401 ataaaaacgt tatcgatact gcattgagca aatttacctg gtttgccagc gaccgtttta
    11461 cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc
    11521 cgcagtctga tcgcgaaatg ggtaataaat cctatggtct ggcatgggat atggatacgc
    11581 agctcgcatg ggccaaacta cgtaccaccg ttggttggga tcatattagt gattataccc
    11641 gtcacgatca tgacatctgg tacaccgaac tttcatgtac atatggtgat attacagggc
    11701 gttgcacccg tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa
    11761 cacgcctgga ctggcaaaaa ttcgccgtgg gtaatgtttc gcatcaaccc tacttcggcg
    11821 cggaatacat ctattccgat gcatggactg aacgccataa ccagtctgaa tcctatgtga
    11881 ttaatgctgc cggaaagaaa actaaccata ccatttacca taaaggtaaa ggcagcctgg
    11941 gaattgacaa ctacacactg tatatggcgg atcgcattag ctggcgtaat gtgtcattaa
    12001 tgcccggcgt gcggtatgac tatgacaact atctgtcaaa ccacaatatc tccccgcgct
    12061 ttatgacgga atgggatatt tttgctgatc aaacctcaat gattacagca ggttataacc
    12121 gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat agctggacgg
    12181 aatcggtatc aggtaataaa accctgacgc gttatcagga tttgaaaaca ccttataacg
    12241 atgaactggc aatgggattg cagcaaaaaa tcggtaagaa cgttattgcg cgcgcaaact
    12301 atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta
    12361 aaaccaccat tactgaatat aacaacgacg gcaaaaccaa aacgcattcg ttcaacctca
    12421 gttttgaact ggccgaaccc ctgcatatcc gccaggtaga tattaacccg caaattgtct
    12481 ttagctatat caagagcaag ggtaacttgt cgttaaacaa tggttatgag gagagcaata
    12541 ccggtgataa ccaggtggtt tataacggca atctggtttc ttacgatagc gttccagtgg
    12601 cggattttaa taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg
    12661 ggttggtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatcctgg
    12721 gtaagacgaa tgcgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa
    12781 aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac
    12841 aacaaaacct gacgatcagt gccgatattc tcaatgtact ggatagcaaa accgcggttg
    12901 atacaacgaa taccggtgtg gcgacctacg ccagtggccg tactttctgg cttgatgtca
    12961 gcatgaaatt ttaatagttc ggattttact taaccgggta aattcgcccg gttttcgcat
    13021 ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc tgttgctccc
    13081 cgggcggctg attgccgccg ccttaccgca ggatgaaaag ttaattaccg gacaactgga
    13141 caatggcttg cgatatatga tttatccgca tgctcaacca aaggatcagg taaatttatg
    13201 gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg tggctcattt
    13261 tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag tcatcgaaac
    13321 atttgagtca atgggcctgc gttttggtcg cgatgttaat gcctatacca gctatgacga
    13381 aacggtgtat caggtgagtt tgccgactac gcagaaacaa aatctgcaac aagtgatggc
    13441 aatcttcagt gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg
    13501 tggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc
    13561 gcgccgccct ttcctgctgg ctaatacccg taatttagac cgtgaaccta tcggcctgat
    13621 ggatactgtc gccacggtca caccggcaca attgcgccaa ttttatcaac gctggtatca
    13681 accaaataat atgaccttta tcgtggttgg cgatatcgac agtaaagaag cgctggcgct
    13741 gataaaggat aatttaagta agcttccggc taacaaagca gctgaaactc gcgtctggcc
    13801 gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa accgggtgaa
    13861 cggcatcgca ctctattatc gcctgccaat ggtacaagtg aacgatgagc aaagctttat
    13921 cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc aggaacgcat
    13981 acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta aaattgcacc
    14041 cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc aggatgctgc
    14101 gaatgcatta atggcagagt tggcaaccat tgatcagcat ggtttttctg ctgaagaact
    14161 cgatgatgtc aaatctaccc gcctcacctg gctgaaaaat gcggttgatc agcaagctga
    14221 acgtgattta cgtatgctga ccagtcgcct ggcatcaagc tcattaaata atacgccgtt
    14281 cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca
    14341 aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat
    14401 ggtaaacaat gagcttgccg ccaaaaaagc attgtctcct gcggctatcc tggcgctgga
    14461 aaaggagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt
    14521 aacaatagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct
    14581 gacatcatta acactttcca atggtgccag ggttattctg gcaaaatccg cgggtgaaga
    14641 gcaaaagcta caaattattg ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca
    14701 aaaatcactt atcgcgctgg caaataaagc agttagcgga agcggcgttg gcgaactctc
    14761 ctcttccagc ctgaaacgct ggagtgcgga aaattcggta accatgagca gtaaagtcag
    14821 tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac ctggttttca
    14881 gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg catcgctaca
    14941 aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca
    15001 gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat
    15061 tgcacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc
    15121 ggatatcacg tttgtcattg tcggtaatgt cgcacaagac aaactcgtgg cgttaattac
    15181 gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac
    15241 tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaacctg tggcacaggt
    15301 ttcacagtgg aagcgttatg attcccggac acctgttaat ctggcgacgc gtatggcgat
    15361 cgatgctttt aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg aacaggcatc
    15421 aggagcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca
    15481 tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga
    15541 agtgatggtt aagcgtctgg ctaaagggat cagtgagcaa gaactgaatg aataccagca
    15601 aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacactat
    15661 tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt
    15721 gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt
    15781 aaatacttat actggagtat tattgccaaa ataataacag ccccgtcaac acatcgttgg
    15841 cggggatttt agcaatattc gctattttta tgtaataatt ttataaa
//
LOCUS       DAPGRN010000109        15553 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPGRN010000109 DAPGRN010000000
VERSION     DAPGRN010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.111"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(173..364)
                     /locus_tag="RHW33_003674"
     CDS             complement(173..364)
                     /locus_tag="RHW33_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647135.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVV
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(361..783)
                     /locus_tag="RHW33_003675"
     CDS             complement(361..783)
                     /locus_tag="RHW33_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV1647136.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEYGADVSVVHGVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            complement(830..1132)
                     /locus_tag="RHW33_003676"
     CDS             complement(830..1132)
                     /locus_tag="RHW33_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1647137.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHLECNAIYAALD"
     gene            complement(1672..2442)
                     /locus_tag="RHW33_003677"
     CDS             complement(1672..2442)
                     /locus_tag="RHW33_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647138.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVRKARAEIRSMADALAKIRK"
     gene            complement(2487..2921)
                     /locus_tag="RHW33_003678"
     CDS             complement(2487..2921)
                     /locus_tag="RHW33_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV1647139.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNTREND"
     gene            complement(2935..3156)
                     /locus_tag="RHW33_003679"
     CDS             complement(2935..3156)
                     /locus_tag="RHW33_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647140.1"
                     /translation="MNYAGHEKLRADVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            complement(3157..3840)
                     /locus_tag="RHW33_003680"
     CDS             complement(3157..3840)
                     /locus_tag="RHW33_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV1647141.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACIEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPPNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3916..4227)
                     /locus_tag="RHW33_003681"
     CDS             complement(3916..4227)
                     /locus_tag="RHW33_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647142.1"
                     /translation="MNSDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLAPVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(4224..5126)
                     /locus_tag="RHW33_003682"
     CDS             complement(4224..5126)
                     /locus_tag="RHW33_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV1647143.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATDEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(5864..6835)
                     /locus_tag="RHW33_003683"
     CDS             complement(5864..6835)
                     /locus_tag="RHW33_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDV1647144.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(6835..8001)
                     /gene="sopA"
                     /locus_tag="RHW33_003684"
     CDS             complement(6835..8001)
                     /gene="sopA"
                     /locus_tag="RHW33_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDV1647145.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(8589..9344)
                     /gene="repE"
                     /locus_tag="RHW33_003685"
     CDS             complement(8589..9344)
                     /gene="repE"
                     /locus_tag="RHW33_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDV1647146.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(10066..10872)
                     /locus_tag="RHW33_003686"
     CDS             complement(10066..10872)
                     /locus_tag="RHW33_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV1647147.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(10873..11178)
                     /gene="ccdB"
                     /locus_tag="RHW33_003687"
     CDS             complement(10873..11178)
                     /gene="ccdB"
                     /locus_tag="RHW33_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDV1647148.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(11180..11398)
                     /gene="ccdA"
                     /locus_tag="RHW33_003688"
     CDS             complement(11180..11398)
                     /gene="ccdA"
                     /locus_tag="RHW33_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDV1647149.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            12026..12256
                     /locus_tag="RHW33_003689"
     CDS             12026..12256
                     /locus_tag="RHW33_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV1647150.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            12253..12669
                     /locus_tag="RHW33_003690"
     CDS             12253..12669
                     /locus_tag="RHW33_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV1647151.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            12744..14309
                     /locus_tag="RHW33_003691"
     CDS             12744..14309
                     /locus_tag="RHW33_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1647152.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            14294..15316
                     /locus_tag="RHW33_003692"
     CDS             14294..15316
                     /locus_tag="RHW33_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HDV1647153.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT         3840 a         4267 c         3932 g         3514 t
ORIGIN      
        1 catcaggaac agttccataa cataagaaag aaacactcct gagtcagaca catcacagac
       61 gggaagggta acgggcgacg cgtcagcgtg gcccgcttcc cttccggcag gcgtcagcct
      121 gccaggggtt ttcgcgcgga ctgacaggcg gcctccaccg cctgcccctg tatcacctgt
      181 ttctgctacc ctgtaagatg tagtgaccgc ccaggaaaac cgcctgctga ttcaccacaa
      241 aatcgcagat ttcatcaaca atctgcgcgg tggtgagcgg tttctgcccc atttcacgac
      301 gccgcgcatt aatcacggcc ctgtaattac gcagaatttc gcgggtttct gtgctgatat
      361 tcatgacatc gtctccccgt ccagcgcctg taacacaacg tccattgctt cgcgttcctc
      421 cctgacgaat gcgtcaccgt cgcccccgtt ctcactcccg acggcataca ggcgcttcat
      481 ttcactgcct gccagcgcca tcactttttc cagcatcacc gccgggactg tcacctgacg
      541 gctggcgcgc ttttcttcca tcagttcacg aacaacgccg tgaacaacgc tgacatccgc
      601 accatattca aaggcatcgt ccagccgctg catgacggtt tccagctcat cgtctgtcag
      661 actccagtcc tgggctatgt gacgcacatc cccacgggtc agcactacgg caaaaacgca
      721 ttcactgtcc ggcacgtctg tatccagcac ttcgcggata atttctttaa cagtacagta
      781 catgttctcg tctcctttac aggggcggca ttgccgcccc gtccgttaat cagtccagag
      841 ccgcgtaaat cgcgttgcat tcgaggtgaa attcgatgtg acgccacaac tgcgcatcgc
      901 gcatccggta aagctgggtc agtcctgcat ccccgctgtc gtggtacagc cacaactggc
      961 ggttaatcac aagggaggtc aggatgatgc ctgcggcatc agcactcacg gtacggtcaa
     1021 accagtttgc accgttcacc atgtggaaac ggtcgccatc cggcatcatg taaccgccac
     1081 cgtccggcag gcggatgaag tgccagaagc ctccctgata ttccggcatc attttttgcg
     1141 ccagcgcgta cacatacgcc tcggcataca gaaaatcgtt gccaaacaga tacggcagaa
     1201 aggagagacg gtcgcactct tcaacattca gagtgacagg tttcgcatat tgcatgatgt
     1261 tttcctccgt tgttcacata ttctgcgaca ccgggcggtg ccgtgcctcc ataaaagccg
     1321 ggcgaacagg cggggatgca ggggcgcaag ggaagccgca gcctgacgca cacggctgac
     1381 gggagttgca gggtgagacc gcgaaatctg agcgggtgaa cgaccggaaa atcacggcag
     1441 acgggaagga gggaccgggt gaccgcagcg gcgaagcgta cccttgcatc aacgccagcc
     1501 cggctaagat ggaggcacgg cacagcccgc acacgacgct gaggccacat aactgaaaaa
     1561 ccacaaggcc atgccctgca tggccgggta aagcggagcc ggcggcgact tcctttgtgc
     1621 tctgtccggg gaccggcagg aaaaaggcgg ggatttaccc tgccaacgtc atcatttccg
     1681 gatttttgcc agggcatccg ccatgctgcg gatttcggcc cgggctttcc ggaccacctc
     1741 ccgcagaaca ccacggtcaa accagcggcg gggcggttca tccggtatac gcagcacatc
     1801 gcaggccagc ccactggaca ggatatgctc aatacaatcg ctgaccaggt acatttccac
     1861 cgacgaaaaa cgtgctcccc ggttcacgat ggcatcgagc accacgggca ggttttcgtc
     1921 atcttcatcg agccagcagg ggtgatcgct ttccttcaca accagacgaa agcggcgcgt
     1981 ctggcgggtc agcaacgtct gaccggtggt ggtcagcgcc gcccggatac gccgcgcatc
     2041 gtctgccacc tggtaacaga tgcttcgtat tgcatcgcgc accgccgcaa gtacagccct
     2101 gatttcgtct tctgccagcc actcaccggc atacgggaaa gacacctgcg ttccgtctgc
     2161 cggaagtacc ggcagcgact gccactgact gccggacaga acaagttccg ccccggttcc
     2221 ggtcagtttc tgaatggcgg aggccaggtg cggcggcagt acgccgccgt gacggttgat
     2281 aagtaacgtc gccagccggg gcgcatccag ccgcacgtaa atgctgatcc agcccggatg
     2341 gttcggattt cgctggtcaa catccgtctc ttccggccag ccctgcgcca gcagcaggtt
     2401 acgggcgtgg cgacaaatca gtgcattcag cgtttcgttc atgattcatg gtctccctga
     2461 atcgccgccc gcaggcggcg ataacatcag tcgttttcgc gtgtgttgtt tttcagcagg
     2521 gtgcgggcct gattaatcac cgcctgacgg cgggtgacgc attccgggac ggcgaggcca
     2581 tgatcccggg cggcccattc acgtttccac aatgcctgtt cagcggtctg aatcagggac
     2641 gccagcagtt cagcagggac ggtcacctgg cggttttcgc tcacgttatt gatgatctcc
     2701 atcgccacgc cggaagaaat accggattca gtccgctggt cttccgggat gtcctccagg
     2761 cgcgccagga cggttctgat ttcatgatcg gacagggaaa tatccattcc gtcagcgagg
     2821 gcctgaatct cttccgggga ccagataacc agcatcagcg gcatgtctcc cggatacttt
     2881 actgccagct cccggcatag cgtttcgcat gttccgtaca ttctcgtctc cttctcaatc
     2941 tttaaagcac gcatccagtt ccagaatttc ggtatatgcc tccatcagga ggcggtttgc
     3001 gcgaaacagc gtctgacgca ccagacgttc cggcagggtg tcggcattaa agctgtaccg
     3061 ctgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca cctccgccac
     3121 gtcggcgcgc agtttttcgt gtccggcata gttcatttac gcagcctccg gcataaacca
     3181 gtcatcattc gcggccccct gctgcatggc ccgttgcacg gcggcaaggc gttgctgccc
     3241 ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg actggagggc
     3301 ggcgacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt tcgggtgtgt
     3361 gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt gatgacgatt
     3421 gcccgaatat ttccagccca gcacgtccgg cagcgggttt ggtggcagac gtggacggcc
     3481 ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct tcgatgtgta
     3541 gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc aggcggccat
     3601 aaagcgatcg acgcggttcc agccgtagaa gctgaccatc agcgcatctt ttttgagtac
     3661 gcgaaacatt tcaatacagg ccggttgcag ccattcatcg gttttatcgc cggcaatggt
     3721 acgcccggaa cggtcacgga aaccgacgag atacggcggg tcggtgagga tgaaatcgac
     3781 agcattgtcc ggaaacgtgg ccataactcg cacgcagtca cccaggataa aacgggacat
     3841 aaaagcctcc ttctcttaca gaaggcaccg ggagggcacc cctgtggtgc ccgttccccg
     3901 cggcctgtgg gctgttcacc gcgtccggta acgggtcagg ccgaccgtgg aataccgcag
     3961 ccgtcgtctt tttgacggca aggctatgcc gcgaaagcgg cttgaagggc gttcatcaga
     4021 aaagggcgac gggctggcgt acactgtcac cggtgccagc cgggagcggt aaaagcgccc
     4081 gtcccgtgcc cgccggacgc gggacactga ccacaggcag cgtttatgcc gcgccgcgtc
     4141 agccggcgca gtaaaaagag gagcacggcg acatcctttg tcggaaaaga aacgggcggt
     4201 tgcccgcccg tctccgtcag aattcatcca ccgtaatgcg ccaccttgtc gacaatccac
     4261 gcaggtccgg tgacttccgg cagctcatcg tcatctgtcg gggaagacca ttccagttcc
     4321 ccccacagga catcaacaag ctctccacgc tcatatcgct gccagccaca gaaattgcag
     4381 ccctgctcgg cataccagtg ctccagtgtg caactgaaac ggcggcttaa ttctgcaata
     4441 acgtcgcttt ccggctgaca ccacggcgtg tcgaaatcaa cctgaatgaa gttgtctccc
     4501 tgactgctga tattcacctc ataccccaca ggccacttca cgccatactg ttccgtatac
     4561 cagtgatacg cagaaggaac accgttcaga acaccaccgt taaagccatt cacttcaaca
     4621 tccaggcggg tgggcagaac cgtcagcagg tcaaacggca tggttttttc cggcagtaca
     4681 ttgtcacaca gacggttcca ccacaccgat acatcctcgt tactccagaa gccacaccag
     4741 tcacctcttt tggcggtgaa atgcacggtg atggtttccc tgacctcatc cggtaatcct
     4801 tcccagcgac gctggccagt accactctgc agccagagtt catgcagcat caggcagttt
     4861 tgttcatcca gtagcacacc gttctgcagg tgtgtcagcc agcgggtgaa cgcgatattc
     4921 tccggcgata caacaccacg cccggcagcc gtcagtccgg ggcatggttc aaaccgcaca
     4981 tcttcagtgg tctgcagaag tccggcacta ccggccagaa acagctgaat accttcatct
     5041 gtggcgcggc gataaagcgg tgtgactgca ccgctggcca gtcgtttaat ctcagcgatc
     5101 tgtgctggtt caccagaaaa atgcatacga tttgagcacc agtttggcat gggaattctc
     5161 ctctcacatg cggcctccat caggccgtgc cgcacctgta accgggcgac cgcatacgaa
     5221 tgcaggtgcg gccccgaagc cgcagcgcgt ggaggaggaa cgacgaaacg tgaccgggtg
     5281 aggaggccgg cgacagcgac acacttgcat cggatgcagc ccggttaacg tgccggcacg
     5341 gcctgggtaa ccaggtattt tgtccacata accgtgcgca aaatgttgtg gataagcagg
     5401 acacagcagc aatccacagc aggcatacaa ccgcacaccg aggttactcc gttctacagg
     5461 ttacgacgac atgtcaatac ttgcccttga caggcattga tggaatcgta gtctcacgct
     5521 gatagtctga tcgacaatac aagtgggacc gtggtcccag accgataatc agaccgacaa
     5581 cacgagtggg atcgtggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg
     5641 tcccagacta ataatcagac cgacgatacg agtgggacca tggtcccaga ctaataatca
     5701 gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac gatacaagtg
     5761 gaacagtggg cccagagaga atattcaggc cagttatgct ttctggcctg taacaaagga
     5821 cattaagtaa agacagataa acgtagacta aaacgtggtc gcatcagggt gctggctttt
     5881 caagttcctt aagaatggcc tcaattttct ctatacactc agttggaaca cgagacctgt
     5941 ccaggttaag caccatttta tcgcccttat acaatactgt cgctccagga gcaaactgat
     6001 gtcgtgagct taaactagtt cttgatgcag atgacgtttt aagcacagaa gttaaaagag
     6061 tgataacttc ttcagcttca aatatcaccc cagctttttt ctgctcatga aggttagatg
     6121 cctgctgctt aagtaattcc tctttatctg taaaggcttt ttgaagtgca tcacctgacc
     6181 gggcagatag ttcaccgggg tgagaaaaaa gagcaacaac tgatttaggc aatttggctg
     6241 tgttgataca gcgggtaata atcttacgtg aaatattttc cgcatcagcc agcgcagaaa
     6301 tatttccagc aaattcattc tgcaatcggc ttgcataacg ctgaccacgt tcataagcac
     6361 ttgttgggcg ataatcgtta cccaatctgg ataatgcagc catctgctca tcatccagct
     6421 cgccaaccag aacacgataa tcactttcgg taagtgcagc agctttacga cggcgactcc
     6481 catcggcaat ttctatgaca ccagatactc ttcgaccgaa cgccggtgtc tgttgaccag
     6541 tcagtagaaa agaagggatg agatcatcca gtgcgtcctc agtaagcagc tcctggtcac
     6601 gttcattacc tgaccatacc cgagaggtct tctcaacact atcaccccgg agcacttcaa
     6661 gagtaaactt cacatcccga ccacatacag gcaaagtaat ggcattaccg cgagccatta
     6721 ctcctacgcg cgcaattaac gaatccacca tcggggcagc tggtgtcgat aacgaagtat
     6781 cttcaaccgg ttgagtattg agcgtatgtt ttggaataac aggcgcacgc ttcattatct
     6841 aatctcccag cgtggtttaa tcagacgatc gaaaatttca ttgcagacag gttcccaaat
     6901 agaaagagca tttctccagg caccagttga agagcgttga tcaatggcct gttcaaaaac
     6961 agttctcatc cggatctgac ctttaccaac ttcatccgtt tcacgtacaa cattttttag
     7021 aaccatgctt ccccaggcat cccgaatttg ctcctccatc cacggggact gagagccatt
     7081 actattgctg tatttggtaa gcaaaatacg tacatcaggc tcgaaccctt taagatcaac
     7141 gttcttgagc agatcacgaa gcatatcgaa aaactgcagt gcggaggtgt agtcaaacaa
     7201 ctcagcaggc gtgggaacaa tcagcacatc agcagcacat acgacattaa tcgtgccgat
     7261 acccaggtta ggcgcgctgt caataactat gacatcatag tcatgagcaa cagtttcaat
     7321 ggccagtcgg agcatcaggt gtggatcggt gggcagttta ccttcatcaa atttgcccat
     7381 taactcagtt tcaatacggt gcagagccag acaggaagga ataatgtcaa gccccggcca
     7441 gcaagtgggc tttattgcat aagtgacatc gtccttttcc ccaagataga aaggcaggag
     7501 agtgtcttct gcatgaatat gaagatctgg tacccatccg tgatacattg aggctgttcc
     7561 ctgggggtcg ttaccttcca cgagcaaaac acgtagcccc ttcagagcca gatcctgagc
     7621 aagatgaaca gaaactgagg ttttgtaaac gccaccttta tgggcagcaa ccccgatcac
     7681 cggtggaaat acgtcttcag cacgtcgcaa tcgcgtacca aacacatcac gcatatgatt
     7741 aatttgttca attgtataac caacacgttg ctcaacccgt cctcgaattt ccatatccgg
     7801 gtgcggtagt cgccctgctt tctcggcatc tctgatagcc tgagaagaaa ccccaactaa
     7861 atccgctgct tcacctattc tccagcgccg ggttattttc ctcgcttccg ggctgtcatc
     7921 attaaactgt gcaatggcga tagccttcgt catttcatga ccagcgttta tgcactggtt
     7981 aagtgtttcc atgagtttca ttctgaacat cctttaatca ttgctttgcg tttttttatt
     8041 aaatcttgca atttactgca aagcaacaac aaaatcgcaa agtcatcaaa aaaccgcaaa
     8101 gttgtttaaa ataagagcaa cactacaaaa ggagataaga agagcacata cctcagtcac
     8161 ttattatcac tagcgctcgc cgcagccgtg taaccgagca tagcgagcga actggcgagg
     8221 aagcaaagaa gaactgttct gtcagatagc tcttacgctc agcgcaagaa gaaatatcca
     8281 ccgtgggaaa aactccaggt agaggtacac acgcggatag ccaattcaga gtaataaact
     8341 gtgataatca accctcatca atgatgacga actaaccccc gatatcaggt cacatgacga
     8401 agggaaagag aaggaaatca actgtgacaa actgccctca aatttggctt ccttaaaaat
     8461 tacagttcaa aaagtatgag aaaatccatg caggctgaag gaaacagcaa aactgtgaca
     8521 aattaccctc agtaggtcag aataaatgtg acgaaccacc ctcaaatctg tgacagataa
     8581 ccctcagact atcctgtcgt catggaagtg atatcgcgga aggaaaatac gatatgagtc
     8641 gtctggcggc ctttcttttt ctcaatgtat gagaggcgca ttggagttct gctgttgatc
     8701 tcattaacac agacctgcag gaagcggcgg cggaagtcag gcatacgctg gtaactttga
     8761 ggcagctggt aacgctctat gatccagtcg attttcagag agacgatgcc tgagccatcc
     8821 ggcttacgat actgacacag ggattcgtat aaacgcatgg catacggatt ggtgatttct
     8881 tttgtttcac taagccgaaa ctgcgtaaac cggttctgta acccgataaa gaagggaatg
     8941 agatatgggt tgatatgtac actgtaaagc cctctggatg gactgtgcgc acgtttgata
     9001 aaccagggaa aagattcata gcctttttca tcgccggcat cctcttcagg gcgataaaaa
     9061 accacttcct tccccgcgaa actcttcaat gcctgccgta tatccttact ggcttccgca
     9121 gaggtcaatc cgaatatttc agcatattta gcaacatgga tctcgcagat accgtcatgt
     9181 tcctgtaggg tgccatcaga ttttctgatc tggtcaacga acagatacag catacgtttt
     9241 tgatcccggg agagactata tgccgcctca gtgaggtcgt ttgactggac gattcgcggg
     9301 ctatttttac gtttcttgtg attgataacc gctgtttccg ccatgacaga tccatgtgaa
     9361 gtgtgacaag tttttagatt gtcacactaa ataaaaaaga gtcaataagc agggataact
     9421 ttgtgaaaaa acagcttctt ctgagggcaa tttgtcacag ggttaagggc aatttgtcac
     9481 aacaccttct ctagaaccag catggataaa ggcctacaag gcgctctaaa aaagaagatc
     9541 taaaaactat aaaaaaaata attataaaaa tatccccgtg gataagtgga taaccccaag
     9601 ggaagttttt tcaggcatcg tgtgtaagca gaatatataa gtgctgttcc ctggtgcttc
     9661 ctcgctcact cgagggcttc gccctgtcgc tcgactgcgg cgagcactac tggctgtaaa
     9721 aggacagacc aaacatcatg gttctgtgtt cattaggttg ttctgtccat tgctgacata
     9781 atccgctcca cttcaacgta acaccgcacg aagatttcta ttgttcctga aggcatattc
     9841 aaatcgtttt cgttaccgct tgcaggcatc atgacagaac actacttcct ataaacgcta
     9901 cacaggctcc tgagattaat aatgcggatc tctacgataa tgggagattt tcccgactgt
     9961 ctcgttcgct tctcagtgga taacagccag cttctctgtt taacagacaa aaacagcata
    10021 tccactcagt tccacatttc catataaagg ccaaggcatt tattctcagg ataattgttt
    10081 cagcatcgca accgcatcag actccggcat cgcaaactgc acccggtgcc gggcagccac
    10141 atccagcgca aaaaccttcg tgtagacttc cgttgaactg atggacttat gtcccatcag
    10201 gctttgcaga actttcagcg gtataccggc atacagcatg tgcatcgcat aggaatggcg
    10261 gaacgtatgt ggtgtgaccg gaacagagaa cgtcacaccg tcagcagcag cggcggcaac
    10321 cgcctcccca atccaggtcc tgaccgttct gtccgtcact tcccagatcc gcgctttctc
    10381 tgtccttcct gtgcgacggt tacgccgctc catgggtatt ttcagtgttg ccaccatcgt
    10441 ctgcagctgg ctgacgtacc aggagtcaga gagcggaacc agccggtgag tctgctgacc
    10501 ggcgggcatt ctccccgccg tcctggcagc tttttcggtc cgttgtttca gggtcgcaag
    10561 ctgcacaaac ggatacggag gcgtgagcga aaaatccccc cgcgtcagcg ctagtgcttc
    10621 attaatacgc gcgccggtgt tccacagagt ggccagcagc atcttgcggc gcagatccgg
    10681 gacataatga agcagggcac tcacctcggg agccagcaga tatttcggca gttcatcatg
    10741 aaccatcgac atctggcgga gtgccagagc tgccggataa tcaatggcaa caggaagttg
    10801 tgcagactgt ccggcagaat acactgccgg taccatggct gcagactggc tgtgtataac
    10861 ggagcctgac atttatattc cccagaacat caggttaatg gcatttttga tgtcattttc
    10921 tcggtggctg agatcagcca cttcctcccc gataacggag accggcacac tggccatatc
    10981 ggtggtcatc atgcgccagc tttcatcccc gacatgcacc accgggtaaa gctcacggga
    11041 gaccttatct gacaacagac gtgcactggc cagggggatc accatccgtc gcccaggcgt
    11101 gtcaataatg tcactctgga catccacaaa cagacgataa cggctctctc ttttataggt
    11161 gtaaacctta aactgcattt caccagtccc tgttctcgtc ggcaaaagag ccgttcattt
    11221 caataaaccg ggcgacctca gccatccctt cctgattttc tgcctgccag cgttcggcac
    11281 gcagacgacg ggcttcattc tgcagggtgg tgcttaccag cccggagata ttgacatcat
    11341 atgccttgag caactgatag ctgtcactgt cgacggtgac tgtaatgcgc tgcttcataa
    11401 tgcacctcat cttacatact ctctgtacac cattaagtat acaccagtat tacgctataa
    11461 catgcctccg gcaacggaat attgttatcc agctattatt aacaccacca tcaataactg
    11521 acctgaaaag taatcatgac aataataacc tgcaatgtag taaccatgat aaattacagg
    11581 atgactgttt ttctatgatc cgatttttat cggattcagg attttccgga agtgaatcag
    11641 gatcagtgaa taagcgaagc gatcttttct gccgctgatg actggctcgg gaacacggga
    11701 agaaacacaa catacagtgt catgtcagcg gagcgggaca ccacatcctg tgaaagcgga
    11761 ccagcgggcg gcagcgaagg atccgcggat ccggaaccac cagaaaccgg atctgcacgg
    11821 ggaaagagag aaaataacat aggctgaaca tatccatatg cctgacatct gcatattcat
    11881 ggtatacgca tatcctcacc atctgcatat gctgaatcta tacatatgcc taacatctgc
    11941 ctatactaaa catatgcaca cacccggtgc aatgaacatc attccggata tgcatcacat
    12001 atccacactt acccgggaga gaatcatgag aaccgtatcc atttttaaaa atggcaacaa
    12061 ccgtgctatc cgccttccac gcgatctgga ttttgagggg gtgagcgagc tggagatcgt
    12121 ccgggaaggg gacagcatca ttctgcgccc cgtccggccg acctggggct cattcctgga
    12181 gctagaaaag gccgatccgg actttatgac ggagcgcgag gacgttgtca gcgacgaagg
    12241 acgagttaac ctgtgaacaa gatctatatg ctcgacacga acatctgctc gttcatcatg
    12301 cgcgagcaac ctgaagcatt gcttaagcac ctggagcagt cggtgctgcg cggtcaccgt
    12361 atcgtggtct cggccatcac ctactccgag atgcgcttcg gtgccaccgg tccgaaggcc
    12421 tcgccacgcc acgtgcaact ggttgacgcg ttctgtgaga ggctcgatgc cgtcctgccg
    12481 tgggaccgcg ccgcggtgga tgcgaccacg gagattaagg tggcactgcg tctcgccggc
    12541 acgccgatcg gtccgaacga cacggcgata gccggacacg ccatcgctgc ctgtgccatt
    12601 ctggtgacga ataatgtgag agagtttgag cgggtaccgg gtcttgtgct ggaagactgg
    12661 gtaagataac gctgtaaaac aaaacaggcg gtcaggcgcg gccggccgct ctttcaggca
    12721 gcggtatata aaggaataaa aaaatgccac tgatcacgcg gcttatgctg caaaacttca
    12781 aaaagtttcc ggaactggac ctgcgcttca gcaacgatcg taacattctg gttggggaca
    12841 atgagtccgg gaaaagcact attctgctgg ctcttgacct tgtgctgagt gacagcaggc
    12901 atcgggttga ggcgctgggg gtggaatcgc tcctgtcaca gtcagcagtc aggcattttc
    12961 aggaaggtga gcgccgtgcc gatcagttac ccgtgctgac cgctgacgtt ttcctgagta
    13021 atgggggaga gcctgacctg aacggcaggc aaaatctggc gggtatcgat gcggatggtc
    13081 tcagaatgcg cattgctccc atgatggagg aatacggtca ggatatacac catgttttgc
    13141 agcaggatcc tgataatttt ccctatgaat actattccgt gcagttcagc acattctccg
    13201 gtggtcattt cgcaggcttc agacgctatc tgcgccatct gttcttagac agtgcacgca
    13261 ttgataacga gcatgccgca caggagtata cgcgcacagt ttacagcgtc aatgtgccgg
    13321 tggccgatcg gtaccggctt gaaaacagtt accggcaaca gaaaatgcac ttctgcgccc
    13381 ggcatctgtc cgccataaac gacacgctga aaacgtatca gtttggcgtg cgttccggcg
    13441 ctaaatcagg tcttgaggcg aacctggaca ttactgagga cggtatttcc attcgtcacc
    13501 gcggaaaagg aagacagtgt ttcatcaaaa cggagtttgc gctgcagcgg catcagcagc
    13561 aggggggaga gatccacgcg ctgttgcttg aggagccgga gaatcatctc agccatgtca
    13621 gtatgaagag gctggttaat cagcttgcca cggaacggca gacgcaggtg tttattgcca
    13681 cacacagtag ccatatctcc tcccgcctcg acctacgtaa ggctattctc ctcggagcta
    13741 cccggccggt actgatgaat gaactttccg ctgagactgc cgccttcttc atgaaagcac
    13801 cggataataa cgtgctggaa tttgcgctgg ccagacgcgt gctgctcgtg gagggggatg
    13861 ctgagtttat tctgatcgag gccttttatc accgcctgta tggcagggca ccggaggatg
    13921 acggggttca catcatcgct atcggcggaa cgagtttccg tcgttatctt gaactggccc
    13981 gtcttctgga aaatcgtgtc gcggcccttc gcgacaatga cggcaactat cagcaaaatt
    14041 gcgatgaacg ctatgctgac gtgctttgtt cccgctcccg ggtatttgct gaccacgaca
    14101 acagccgctc cacatttgaa atctgcctgt atcaggataa tgccgacctg tgtgatgcgc
    14161 ttttccgcgg aacccgccgg acactcacgg ttcaggatta catgctggcc aataaggcag
    14221 aagcagcatt ccagctgtta cagttgcacg ccgaaaagct gaccgttcct gattatatcc
    14281 aggaggcact ggcatggata agagagtgat atttgcggtt gccggctcgg gtaaaaccac
    14341 gctgctgata cgtcggttaa gagaggaccg gcggacgctg atcctgacct tcaccgtaaa
    14401 taatgaagca catctgcggg cgcagattat cagacgcttt ggctacatac cgtacgggat
    14461 ccgtgtgatg acctggtttg agtttctgca cggtttctgt tttcgccctt tcctgcagga
    14521 gcaactgtca tcgcgtggtc tgagcttcaa ccagccccct tccagaatac cgcgcacgaa
    14581 tatccggcat tatcaggatc ccgccggacg gctgtatcac cggcggctcg cacacctgct
    14641 gacagcccgg gggttgctgc cagatatccg tacccgactt gcccgttact acgatgagtt
    14701 gttcgttgat gaagtccagg actttgccgg ccatgacttt aattttctgc tggagctatg
    14761 ccgcgcggaa atctcagtgc tgtgctgcgg ggatttttat cagcatacct ttgacacaag
    14821 ccgcgacggt aacgtcaatg ctacgctgca tgaagacata acccgatacg aggctcgctt
    14881 cagggctgcg ggtatcatgg tggattgtga gacgctcagc cggacatggc gctgttcagc
    14941 tacagtgtgt gagttcatta ccggacaact gaacattcgc atttcggctc atggaacaca
    15001 taccacccag attgaaatcg tcacggatga agcgcgttca gcggccctgc acgcggacaa
    15061 cacgatgatc aagctcttct atcgcgaaca tcaccgttac ggatgccact caatgaactg
    15121 ggggggcagt aaaggactcg accactttca ggacgtctgc atcgtgatgg gggccaacca
    15181 ctggatgcgc ctcatccaac agaagctggc agcacttcca ccatcatccc gtaacaggct
    15241 atacgtggcc tgctcacggg cacggggcaa catctatttt attccggaaa gccatcttcg
    15301 gagattcaga aactaagtca gcggcttctc aactgggcac aagccggtaa aaagtagtca
    15361 acgacaaaac ttcaggccga aaaatgaggc tccggtttcg ccggaaaccg gacctgcgtg
    15421 gagggaaacc cgcagatgat atgatattta tcattaatcg cataataaat cctggcctga
    15481 tatttacagg cagagaaata aatcattcga tatatgattt tatcgtcatc agccattaac
    15541 cccccaggaa gcg
//
LOCUS       DAPGRN010000110        15358 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPGRN010000110 DAPGRN010000000
VERSION     DAPGRN010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.112"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            128..598
                     /gene="excA"
                     /locus_tag="RHW33_003693"
                     /pseudo
     CDS             128..598
                     /gene="excA"
                     /locus_tag="RHW33_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
     gene            complement(670..879)
                     /locus_tag="RHW33_003694"
     CDS             complement(670..879)
                     /locus_tag="RHW33_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDV1647154.1"
                     /translation="MSMPPAIANTFLFEMMKSKSKDVTLAAIYALGEGRCQAENITRE
                     LHRLSQSDDMEIKIAAIKALGRIYR"
     gene            1271..1447
                     /locus_tag="RHW33_003695"
     CDS             1271..1447
                     /locus_tag="RHW33_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647155.1"
                     /translation="MNLVDAFVKKVISEPYEEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            2106..2357
                     /locus_tag="RHW33_003696"
     CDS             2106..2357
                     /locus_tag="RHW33_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647156.1"
                     /translation="MSEKWSKEFDSCFQVGSIEEIADALEKRSGSEENALAMLAMTAF
                     TLMTRRGICEMQNVAPDGKGIRLELIGFRENETIPDTLH"
     gene            complement(2429..2581)
                     /locus_tag="RHW33_003697"
     CDS             complement(2429..2581)
                     /locus_tag="RHW33_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HDV1647157.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATL
                     AYEVKR"
     gene            2873..4081
                     /gene="trbA"
                     /locus_tag="RHW33_003698"
     CDS             2873..4081
                     /gene="trbA"
                     /locus_tag="RHW33_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HDV1647158.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPHIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMLSLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            4100..5170
                     /gene="trbB"
                     /locus_tag="RHW33_003699"
     CDS             4100..5170
                     /gene="trbB"
                     /locus_tag="RHW33_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HDV1647159.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLKAARAAIDVNNVAFRKFGFAGTPWVLSDTGWHLPTG
                     ILQETGTLNLFLKTTDSESGHE"
     gene            5163..7454
                     /locus_tag="RHW33_003700"
     CDS             5163..7454
                     /locus_tag="RHW33_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HDV1647160.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTS
                     AHTTSALGRMFITQKSMILARDLGYRLEGTDSDALEVKKYKGRFPYLCFLDEVGAYYT
                     DRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLMQNTGTKFAGRIVSEGSTA
                     RTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELHPGEFFS
                     IFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPE
                     NVSAIIGVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESA
                     LWETAVNTLKTTTREERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNH
                     PTGRPVNGFHHKKTNRPDWDGMY"
     gene            complement(7491..10190)
                     /gene="nikB"
                     /locus_tag="RHW33_003701"
     CDS             complement(7491..10190)
                     /gene="nikB"
                     /locus_tag="RHW33_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HDV1647161.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYIAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFLQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGEFVCTDYGDRVVFRNHHDR
                     NALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAWHNVTGRTGHEDYRITR
                     PDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG"
     gene            complement(10201..10533)
                     /gene="nikA"
                     /locus_tag="RHW33_003702"
     CDS             complement(10201..10533)
                     /gene="nikA"
                     /locus_tag="RHW33_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxosome accessory protein NikA"
                     /protein_id="HDV1647162.1"
                     /translation="MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            10767..11102
                     /locus_tag="RHW33_003703"
     CDS             10767..11102
                     /locus_tag="RHW33_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HDV1647163.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTASKKTDSAR
                     KDGIDMNNN"
     gene            complement(11187..12035)
                     /locus_tag="RHW33_003704"
     CDS             complement(11187..12035)
                     /locus_tag="RHW33_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1647164.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFIGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     SLPPRV"
     gene            12384..12581
                     /locus_tag="RHW33_003705"
     CDS             12384..12581
                     /locus_tag="RHW33_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647165.1"
                     /translation="MQGAPHPARLKKNDGATMACGGHWHTTPAQVKTEKTARHLQSKK
                     VKFLHFFTLIFRADLVVMVIK"
     gene            12615..12866
                     /locus_tag="RHW33_003706"
     CDS             12615..12866
                     /locus_tag="RHW33_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1647166.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELVETAEDV"
     gene            complement(12897..13067)
                     /locus_tag="RHW33_003707"
     CDS             complement(12897..13067)
                     /locus_tag="RHW33_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647167.1"
                     /translation="MTIAERLIQKGFEKGALEVAREIACRLRDMGWTPERIQEATGLS
                     GEELKKLFPDEQ"
     gene            complement(13129..14070)
                     /locus_tag="RHW33_003708"
     CDS             complement(13129..14070)
                     /locus_tag="RHW33_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV1647168.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRQRDMLMCLDNLVRALQDIQDEEQITVLFNYL
                     LNGSEHVTVEFLQTLAQRLPQHEDSIMTLAERLKQEGIQQGIQQGIQQGIQQGVQQGA
                     LQKAREIARELRNAGMPAAQICQLTGLSEAELKNITH"
     gene            complement(14135..14401)
                     /locus_tag="RHW33_003709"
     CDS             complement(14135..14401)
                     /locus_tag="RHW33_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647169.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(14493..14927)
                     /locus_tag="RHW33_003710"
     CDS             complement(14493..14927)
                     /locus_tag="RHW33_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HDV1647170.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            complement(15171..>15358)
                     /locus_tag="RHW33_003711"
     CDS             complement(15171..>15358)
                     /locus_tag="RHW33_003711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647171.1"
                     /translation="VRCLKNQACSDRSGRAAPVAPETALPAHLTVCKATAPDGAVNPS
                     AATPAACFPLWTFRGLE"
BASE COUNT         3898 a         3998 c         3721 g         3741 t
ORIGIN      
        1 cgcccttaga tttatatatt tcataagcat accagtgtta tccattttta ttcttttcgg
       61 agtgctctcc attacatctt caagatatgt gacgcaagaa gattatattt atacttgcgt
      121 ctgtctgttt ttactaattg cccctgcaat attgatgtat tcccgagcaa gctcaagaaa
      181 agacaaaata aaaaaaattg tcgcggagat aaaaaacacc ggtttttatt cacctgacaa
      241 agaatatgag ggactgtctt ttactcaggg cgtctatttt ggcgtggata ccaaaaaagg
      301 cactatgctg tatgctcgtg cttaccctgg aaatataatg gatattatcg gctttgacat
      361 tgacaacttc acacgcactg tgaccgacgc caagacacta gagatttata caaaatacat
      421 aaatatacca atggtctcaa ttccgtcagg atgcatccat cccaaaatga tggcggatac
      481 aatgcatgca atggcagaaa gaggatatga ttaccctgtc gactttcctc gcctaattca
      541 ggagaagcgt aaggagtggg agcagattgc aggcatgcct gtagccgaag tattttaaga
      601 taagctccct ccataacagc cggttcaaat gagccggctt ttgttcatta caacagataa
      661 cttatgccgt taccgataaa ttcgaccaag tgctttaatc gcagcaattt ttatttccat
      721 atcatcactt tgactcaatc tatgtaattc acgagtaatg ttctccgcct gacaccggcc
      781 ttcacctaat gcatagatcg cagcaagtgt aacgtctttt gatttactct tcatcatttc
      841 aaacaagaaa gtattagcta tcgcaggagg catgctcata atcaatcctc ttcatgaaat
      901 aaacaatggc actcacttac aaacaggagt ccactatcgc aagcgactca acttagcaat
      961 atctctcaat ttgaatgaaa aaaccagatg gcatgtgaca gggaaccgtc cgcatgatgt
     1021 ccgtgaccat ttaaccccat aagctaacct tgtcataaac gggcgcagaa cagcctgcca
     1081 gacagacaac aaaacaaaag tccaagataa aacatgaata ccggtatctc aatgcgaaga
     1141 aataatctcc gtagaaaccc ccatgagaaa aatatcatat gggagaaaat cgcaattatc
     1201 acagccagcg cattcattat tttcgctgtg ctctggcatt aattattttc aacacaaaaa
     1261 ggaaaaaaca atgaatctgg tagacgcctt tgtcaaaaaa gtaatatccg agccatatga
     1321 agaatatgga aaatggtgga tagatgttga atacatttca tggggtgttc ccgggaaaac
     1381 aagactgatg tttgagtcaa aggagcaggc actggaagtt aaagaagggt acaaattttt
     1441 aacgtgagat ctctttttgt taaagggcag gaagcggtaa ctccctgccc ttagttacgt
     1501 ttccaggctt atcagtcgac aagttcccgg atcagttcca gaacagcaat cagcgcgttt
     1561 gcaacggcaa ctactgccct gagaacgatg attaacagat taatcatccg cttctccatc
     1621 actggaggaa atcaccgttg cttactctta cattgcctgt cacaacggca ctttcagtgt
     1681 tagttgaaaa gagacttcct cctgccagag cactgaacac agcaccacct gtttgttcag
     1741 ccaggactta aaatcttgca tccatagtcc cgatggatat cacggcagaa gtgtcgccag
     1801 acagagctaa cctgcggatt ataaggatgg ttgctatcaa atgaacagct cgctattata
     1861 agaacgttag ttgaaaagag atttcctccg gtcagggcca cccactctgt aacggaacag
     1921 taaaaaaacc gacctcacca gtcggttttt ttacatctgt tgtttctcaa aatccgcccg
     1981 acatgcccgt cacaccgggg aaactcatgc tctgaatcag aaccaatcat ctcaactttg
     2041 ccgtctcctg aaaaaactgg cacattcagt tcgataacta cccataaata cagagagaaa
     2101 atccgatgtc agaaaagtgg agtaaagaat tcgactcttg ctttcaggtg gggagcatag
     2161 aagaaattgc cgatgctctc gagaaacgca gcggaagcga ggaaaacgcg ttagccatgc
     2221 tggcaatgac agccttcaca ctcatgaccc gacgtggtat ctgcgaaatg cagaacgtgg
     2281 cccccgatgg caaaggcatt cggctggagc taatcggctt cagggaaaat gaaacaatac
     2341 cggatacgct tcattgaaga agaaagggcg caatgaggtc agccttcgca acaaagcggc
     2401 ggcagaaaac ctgccgccgt gttgcccgtt aacgtttaac ttcgtaggct aacgttgcca
     2461 caagcactgt gtttccctgc tggagccgga gtacgcaaag cgaatccctc accatccaga
     2521 caaaacacag aatcgtcaca cagatgacga ttaacatcat taaaaacgtt cgctgtggca
     2581 tcatagcccc tccattgcct tacggcggtt atcgggctac tataatgttg tcgaagcatt
     2641 acagtagagc ccttggttga ttgataaaaa aatctccagg ggctttctgc tgtataccgt
     2701 ccacaaaacg ctttcggcat acagcctcaa gcgcccgcag ctattctaac gactttcgta
     2761 tctgcaacaa tccttttatc acacggctaa ttcctctggt tagctcttat cgcctgcaaa
     2821 taaattttta acctttccgt ttgatttaaa ttccagaaat ggagactttg tcatgtctta
     2881 caatcgacag cctgtcgcag aagatcctat gcagatatgg ggcgctgttg gtgtccttct
     2941 tatcctgctg ctattcgtta tatggttatt cttgccggaa gtggtttacg ccagctgcct
     3001 gattcttcat accttatggg gactggttga ctggggacca tttcataact atgcagcacc
     3061 acgatacaac ctgcttgcca tgaccggtaa caacgccgcc aacataagct atagccagtg
     3121 ggttaacgtc atggagcaga ccataggtat tctgtggatg tacctcttac ctgtgacact
     3181 ctggtgtttg tgggagtggt atcaacatcc gggacaaagt cgtttcaccc gcagaccagt
     3241 agatattacc cgcctgccac acatctttgc ttcgttgtcc cctgctatcg cccctgtact
     3301 ggcagatggt gatccggaaa aactctttca tggtggcaaa cgacctgaac ggcgggttgc
     3361 tctcaccccg gaagcatttg tcgagcagca tactctcatc accaatatgc agctcgatgt
     3421 tgcagctgca agacgatgtt tcatggccca gttaggtaaa cctttaacgt cctggaaaga
     3481 catggcacca catgaaaaag cgctctttgc catatttggt cttcagtatt tcctggatga
     3541 tcgtaaggca gcattaaagc tcatggatac actgaacctc tcatgcagaa ttaaaagcaa
     3601 acgggacagt gggaaattct gtacccctgt ttattcactg gccaaatcag ctttccagag
     3661 agtgattaaa agcaacggag cacagcaatg gctgaaacaa catcgctacg tccgcagtgg
     3721 gctggtctgg ctgtatgcac atgacctgcg gcttacgcca cccaactgga tttggctgaa
     3781 aggcgtggac aggacgctgt tttatgcatt acaccgcgcc aataccacga aaggatttat
     3841 tgaaggcgct ggcgtggtgg ccgttgcacg tgcagaggcc gaagccatgc gttttggtct
     3901 tccctgccct gaaccctgtg ttgatgaagc ggtggaaggc ttacggcggg atatgctcag
     3961 tctcggtctt atctgggatg aaccacaacc agatcgcgac cggaaacgcc gcatcctgac
     4021 caactggtcc ctcaccgacg acatcctgcc ccgcacacct gctacagaca acgaattctg
     4081 attatccgga gaactgaaaa tgacaatcga gtactttgca acacgcattg caaaacatat
     4141 ttccgtgcag gcaatctggc cggatggcag gacagaaatt attgccgtcc ttccacggga
     4201 tgccctgtca gggctggtaa cacgaaataa tcaactgatt catatagcct ttgacgccat
     4261 gggcacccgt aacagagaaa ccgtcctgat tgataaacag gagcataacg ctgaccagat
     4321 acttacagct attgattcct gcctgcgcct tgaatatctg atggaacgcc gcttcagcag
     4381 aacacctttg ttccgtggca taatcgccag cgttgtgctg ttcgtaatgg caacgattgg
     4441 cttctctctg ttccgctatg ttgatcgtgt attctgggat gatacaacgc ctgaagcagt
     4501 acagacagca ggtgagccac gactgctacc ccctcacctg aatcacactg tgccattaaa
     4561 tgaaggcatt caactcccgg ttcccccgaa aaaagacgtc caggtcccgg agaaaacaat
     4621 cacaagcaaa aatccggaag ccgcagctgc gcgacataat ctggctactg ttctgaagcg
     4681 gaacgccgat cggggcatgt ttaccatcaa cctgtcatcc ggtcatgaac gaaccctgtt
     4741 cgcatttctc gatcctgcct gtcctaactg cagacttctg gagccggcct taaaacgcct
     4801 ggccagcgac ttcaatgtcg ttatctaccc ggtttcagtt attggtggag aggaatccac
     4861 cgaccgtgtg gcaccactgc tgtgtgaaaa agatgctcag aaacgtgctg ccggctggca
     4921 ccgactgtat tccgcagata acggaatgat gacgccatca gaagaaacaa cgcctgcaga
     4981 tgaaacatgc ctgaaagcag ccagagctgc gattgatgtt aacaatgtcg ccttccgtaa
     5041 gttcggattt gccggtaccc catgggtgct gtccgacacc ggctggcatc tgcctacagg
     5101 aattctgcag gaaactggca cgcttaattt attcctgaaa accaccgaca gcgagtctgg
     5161 ccatgagtga acatcgcgtt aaccccgagc ttttacaccg tacagcctgg ggaaatcctg
     5221 tctggaatgc actccagagt ctcaatatct atggcttttg cctggtggca agtcttgtgg
     5281 ccagttttat ctggccactt gcattaccgg catgtctgtt gttcacactg ataacaatgc
     5341 tggtatttag tctgcagcga tggcgctgcc ctcttcgtat gcccatgacg ctggaatgtg
     5401 ctgacccgtc acaggacaga atgattaaaa gaagtctttt cagcttctgg ccaaccctgt
     5461 tccagtatga ggtcattctg gaatcaccgg ccagcggcat attctatgtt ggttatcaac
     5521 gcgtcaggga tattggcagg gagttatggc tgagtatgga cgatctgacc cgccatataa
     5581 tgtttttcgc caccaccggt ggcggtaaaa cggaaaccat ctttgcctgg gcgattaacc
     5641 cactttgctg ggcaagagga ttcacccttg tcgatggcaa ggcacagaat gatacggcaa
     5701 gaacgatctg gtatctggcc agacgctttg gacgtgaaga cgatgtggaa gtcattaact
     5761 tcatgaacgg aggaaaatcc cgcagtgaaa tcattctgag tggtgaaaaa acgcgtcctc
     5821 agtcaaatac ctggaatccc ttctgttaca gcactgaggc attcacagcg gagacaatgc
     5881 agtcaatgct gccccagaac gtccaggggg gcgagtggca gtcccgggcc attgccatga
     5941 ataaggcact agtatttggt actaaattct ggtgtgtgcg cgaaggcaaa acaatgtcgc
     6001 tgcagatgct ccgtgaacat atgactctcg aagggatggc caaactttac tgtcgtggcc
     6061 tcgatgatca gtggccggaa gaagctatcg caccgttacg taactacctt caggacgttc
     6121 ccggtttcga cctgtcgctg gtcaggacac catcagcctg gacggaagaa cccagaaaac
     6181 aacatgctta tctctccggg cagttttctg aaactttcag cacattcacg gaagcattcg
     6241 gggatatttt tgctgaagat tccggtgata tcgatatccg tgacagcatt cacagcgaca
     6301 ggattctgat ggtaatgatc cccgctctgg atacatcagc gcatacaacg tctgctcttg
     6361 gacgaatgtt tatcacccag aaaagtatga ttctcgcccg tgatttgggc taccgcctcg
     6421 aaggaactga ttccgatgca ctggaagtca aaaaatataa ggggcgtttc ccttatctgt
     6481 gtttccttga tgaagtgggg gcatactata cggaccgtat tgctgtggaa gccactcagg
     6541 tgcgttcact ggattttgct ctgattttga tggcacagga ccaggaacgt atagaaggac
     6601 agacaacagc cacgaatact gcaacactga tgcagaacac ggggacaaaa tttgccggca
     6661 ggattgtcag cgagggtagt acggccagaa cgctgaaaag cgccgccggt gaagaagcca
     6721 gggcgagaat gaacaacctg cagcgtcagg acggcatatt tggtgaatca tggattgaca
     6781 gcccacaaat cagcatcctg atggaaagca agataaatgt tcaggagttg attgaacttc
     6841 atcctggcga gttcttttct attttccggg gagaaaccgt accatcagca tcattcttca
     6901 tccctgacga tgagaaaagc tgcagcagcg atcccgttgt tataaacagg tatatcagcg
     6961 tggatgcgcc acgcctggac cgtctccgcc gtcttgttcc ccgtactact caacgtcgaa
     7021 taccgtcacc ggaaaatgtc agtgccatca ttggcgtcct gactgcaaaa ccctccagaa
     7081 aaagacgaaa aattcgcaca gagccgcaca cgatcgtgga tacattccag caacgcattg
     7141 caggacgtca ggcggctatg gcaatgctgg aagagtacga cacagatatc aacgcacgtg
     7201 aaagtgcctt gtgggaaaca gcggtgaata cgcttaaaac gaccacacgg gaagagcgac
     7261 gaattcgcta tatcacgctg aacagaccag aactacctga aacaaaagag gaaaatcaga
     7321 tatcagtgag agcagaaaga gcaggtataa acctgttaac gcttccacag gacaataacc
     7381 acccgacagg gcgtcctgta aacgggtttc atcataaaaa aaccaaccgt ccggactggg
     7441 acggaatgta ctgaacagtg gccgcgccgg attctggcgc ggcctttttt ttaacctgga
     7501 gaaggcggtt cccagcgttt atcctgctcc ggcggaggca gtgatgcgtc atcgttgctg
     7561 ttcgccgcga tataatcgcg gtagtagcgt tcacttcctt cacggtgatg atccacgtcc
     7621 ggtcgcgtaa tcctgtagtc ttcgtgaccg gtacggcctg tcacattatg ccaggcaacc
     7681 atttcagcaa agacctggtt aaattgtttg tcgtttcctt ctggttcaaa acccatgcgt
     7741 ggatcacgcc cgaataatac cggtgcaatc aaatccagtt tatctgccag cgcattgcgg
     7801 tcatggtgat tacggaaaac aaccctgtca ccataatccg tacagacaaa ctctcccgtc
     7861 cgacggtcac ggaaacgaac actcccgttc ttctgcaaac gggcctcgag atcaccggta
     7921 ttaccgtata ccggcgttcc gcccggttca caaagcacga cacagcggtc tgttgtcgtt
     7981 gtgcgtgact tatctttacg gcgatcgcga taatcccagc ctctcagctg cgatacagca
     8041 gccctgtctc cctgagcagc ctgaatttcg acccactgac ggtaagaggg tggataccac
     8101 ttcccgtcag caataagttt ctgccgctca tccctgatgt cttctttcag cctgatcaat
     8161 gcctgcattc gctgaacttc tgcaatgtga taatgcagct tacgtaatgc cggatcatcg
     8221 tactgcgcac gaatgtgtga cttccgcaga cggcatgcct gatgaatttc ccgacaacgt
     8281 tccccataac gcagatctgg tttacgccat tgttcacgcc atgccagata gcgggcacgc
     8341 aggtcagcac gggcctcagc tctggcttca cgtcgctgcc ggcgcagcat tgggtcacgt
     8401 ttactgctga ccccgtacct gtcactgaca gccagctccg ggttatagcg actttcaggt
     8461 tgcaccctgt caaacaaatc tgctggggca ctcacaaaag gtccggcctg tggctctgca
     8521 cgccccagag tcagatcagg atgaatgctg ctggccttca ccggcgtttg ctcatgatta
     8581 aaggcatcaa cgacaacaag cccgtgatgt tgtttctgta gcatcagtcc ctgttgtgca
     8641 aacaactcat gacaacgctg ccaggtaaac tcaccctgtg tgatcatttc ttcaatctga
     8701 gggctacaat ggctgatgct gtatccccac agactttctt tgtcagaaaa cagttcgcgt
     8761 ttgatagccc cctgcggccc cgtacctgta cgcatacggt gccattcttt atcggtcgca
     8821 ggacagctct ccatacgacg accacggaag cgttctgttg gcgtaacctg gagaaaaata
     8881 tctgttggta caggctgcca gccaccatcg agttgattca cattgtcgag cgtcagcagt
     8941 gaccatacgc tgtcagcacg aacgggagtc tggttatgat cataaccgtc acagataacc
     9001 aggtgaccat gctgaacacg catccataat cccgcttcag ccagtgtgcg gtgaatagcc
     9061 tggcagttct ccagccgtcc ttcccgtgcc atttccggca gacgttctcc aagatgacga
     9121 caggcatact gctgcagact ttctgtttca gcgatttttt ccgggcgaac atcagctgca
     9181 atgaagtccg gattatagcg tcctggtgct tccacctggc tgaaaatgtc ttttggtact
     9241 ggcgtgtagt cacccatttt tttcatgagt ttttctgcac accaggaagg gccaaacgag
     9301 tcaagctgta caccttccct gttgcgatcc cagccatcca tcaccagaaa ttttccgtcc
     9361 atctgagaca ggtacagacc atcttccgca agacgacgat gcagggataa ccagtcatgt
     9421 acaggttcac tgcgtaaacc agctaccgct gtctgcgcaa tatactcgcg aaaagtttgt
     9481 ttttttccgc gtgtccacgc gttctggcga tcacgttcaa cggcggtttt gcgaacgata
     9541 cgatttcccg gtgcatggac ccagcaaccg ttatccggag caaaaccatg cttcagttca
     9601 agctcacggc aggcacgact gagtttttcc tgactccagg ataaccggtt cagataaccc
     9661 gtttcagggt gtacccggtt aacagccaca tggacgtgca gattatctgt atcagtatgc
     9721 acggcagaga catactgatg gtcggcaagg ccgagtgatt tcagcgtatg acgtacactg
     9781 tcataaatct gttccggacg cgggctttca tgtgactgcc aggaaaggat gtagtgaaaa
     9841 acaggatcag tgtcatcttt tgcatagtgt gcctgccggg caatgtactc catatctgca
     9901 gcggcagttt caagagaagt acagttatga aagcaggtga caccataaaa gttgacccat
     9961 tcgcagccgt ccttcatgac atcaaccagc gccacgaatg actcatttcg aagacgtgtc
    10021 gcataatcaa caaggcgact gaaacggctt cggtgcggtt gttcagcctg cgaagaagaa
    10081 gataaatcca gttcctcatc ggtcatgtca tcccggacag agacgtagga tactaggtct
    10141 tcgaacgaag acttgccgtc ccttctcttt ttcggaatga ctgcattcac aggagaatcc
    10201 tcaaccttcc attaactgtt tacgcaatgt atttgtaagt tcggttatgg caacaagcac
    10261 ttctgcgtat tccttgtcac cggttctttt tccctgaaca aacagatgct tctgcatcct
    10321 tcccagtctc attaactcct tcagaaaagc atcatctgta cgggagttaa ttcgcttatt
    10381 cagtgctgca tttcggatgt aagcagatac agtaagtccg gaatcttcag cttttttcct
    10441 gatggtttca tcctcaacag gagagaatct gagtgtcctg acaactgtct tttgccgtac
    10501 ctcacttttc tttcttaccg cactatcact cacttcaggc tccttacggg gtgtcggggc
    10561 gaagccctga ccagatggca attgtaatag cgtcgcgtgt gacggtatta caattgcaca
    10621 tcctgtcccg tttttcaggc cattatagcc tgttattttt gtgcatcata gtcttatgca
    10681 gacggcagag gcagggcgca gccctgccag gagtcagttt ttgatgataa aatcagactt
    10741 caacatgaaa cggagagaaa gtgccaatgg cttctaaaaa attttactca gatgatgaca
    10801 tccagctggc aaaagctgct ttatcggaat tgccagatct caccgctcaa cggaaaacat
    10861 tgcgtgattt tcttgatgcc attcgtgatg acattattgt tctggtaagg acaaagggtt
    10921 atacactggc agatgttcgt gacacgctca aaaatgcagg gtatgaagta ggagagaagg
    10981 cacttcgtga cattattcgg gaagcagaat cgaaaaagcc atcccgccgt tcttcctcta
    11041 aaacagcatc taaaaagaca gattcagcca ggaaagatgg catcgatatg aacaacaatt
    11101 gatgtgacaa tgtcacatca tgttctgata ttatgatgat tacagaaaaa aagccccggc
    11161 ataaatacca gggcttttct gtttaatcag actcgtggcg gcaatgaacc aggataatgc
    11221 cgtgcaataa tatcattcag tttttcaaca aggtcaggac gcttaaagac aatgtgtacg
    11281 gtccctttct ggtagccacg caccgtaaaa tattcccctt ctaacagctt accgacattg
    11341 tcgccgttcc cgataaattc accgtactgc gccccgatgc tgctccggaa gtcaggaacg
    11401 ttgcgcccct ccagcagata aaacggacgt accaggtcat ccagtgcctc ccgtccgctg
    11461 cagtccagcg taacacgccc gttactccag cggtacagca ggttttcaat aatgattctc
    11521 ttgcccagac ggcagggatt attggttctg taatcccatg acaatttccg gtagacatca
    11581 atcagtccct gttcaaatgt ctgcatctta ctggcattca gatggcgaaa agttgccagt
    11641 acattgtcca gggtgatttc aggacaggtg tcgctcatca gctggctgtt ccactcatca
    11701 cgctgacatg aactcatgaa ggtgtacatc cccgtatccg tcatcaggcg gtcccagatt
    11761 ttccggtcgg caagacgggt gatattcctt ttcgcttctt ccggtttatc catgcaccga
    11821 acggagtcgg tcacgcagtc ttcaaaaccg taaagaaaaa tatcacctga cgcatcaagc
    11881 agaatcctgc gcgctgtcgc caggcattcc atcgcctgca tgaacagtgc aataccttcg
    11941 ttacgctgcg cgatgatgcg gtcgatggcc acagacggaa taacatctgt cgtgccggca
    12001 gtattcaggt cagcagtggg taaagtctga ttcatcacat tgtctcctca tcgtcacgac
    12061 tgccgggtca gccaggtcat ttcccggagg agcgaagctc cttctgccgt ccgggtcttc
    12121 ggggatttcc ccgcagtgga cctttccgac gggggacgct caccacgtca cggggcagtt
    12181 gtccgggggc agacgcaaaa gtttttgacg cctgcggcaa aaagttttgt ggctggtaca
    12241 tctctcccct ggacgactga aacgggatgt ggtcgtgtat gtacccccgg aaagtccatc
    12301 tgcggagaac aggccgcagt gcggcggcag acaggcagag acagctgcgc catcaggcgc
    12361 agtggcctta cacaccgtaa ggtgtgcagg gagcgccgca tccggcgcga ctgaagaaaa
    12421 acgacggcgc aacaatggca tgtggtggtc actggcacac cactcccgca caggtgaaaa
    12481 cagagaaaac agcacgacac ctacaatcaa aaaaagtgaa attccttcac tttttcacac
    12541 ttatttttcg cgctgatttg gtggttatgg tgataaaata aagtgaagtt ttttcacttt
    12601 ttcaaaggtg ttttatgacc ggctgggaac tgcgaatctg gcgaaaaagc atgctctggt
    12661 cacgcgaaaa agcggccaga gaatttggcg tgacacagag aacatggcac gcctgggaaa
    12721 atgcggaaca ggtggatgtc accgtctgga ggaccaccca ggcactttca gtccgtgacc
    12781 tgctgcctca catgcagggg atgcgcaagg cagatatcat caggcgtctg gaaaatgaac
    12841 tggtggaaac ggcagaggat gtgtgagcgt ggttcacagc ctggcagaat gccaggctac
    12901 tgctcatcag gaaacagttt tttcagttct tcaccggaaa gtcccgtggc ctcctgaatc
    12961 cgttccggcg tccagcccat atcccgaagc cggcaggcta tttcccgcgc cacttcaaga
    13021 gcaccttttt caaaaccttt ctgtataaga cgttctgcaa tcgtcatact gttttctctg
    13081 ctcctgctgg caaaaggccc ggcattttgc cgggcctttt ggctctcatc agtgagtgat
    13141 gttcttcagt tcagcttcag aaagtccggt cagctgacag atttgtgctg ccggcattcc
    13201 cgcgttccgg agctcccggg cgatttcccg tgctttttga agcgccccct gctgaactcc
    13261 ctgctggata ccctgctgga taccctgctg gataccttgc tggatacctt cctgcttaag
    13321 acgttctgcc agtgtcataa tgctgtcctc gtgctgcggc agtcgctgcg ccagcgtctg
    13381 caaaaattca accgttacat gttcgctgcc attaagcaga tagttgaaaa gaactgtaat
    13441 ctgctcttca tcctggatgt cctgcagtgc tctgaccagg ttgtccagac acatcagcat
    13501 atcccgctga cggatatgct tcatcagcag cgtcagtgcg gccatccggc gatggttcat
    13561 gatttcattg tcatcaacga cagtgatatc aaccagcgga aacggctttg tgtatatttt
    13621 agccgcaagc gccggattct caaaacagtc cagccagttc atgctgtacg ggtacgggct
    13681 gcgctcaccg tgatagaaca gcaccggaat gaccagtggc aacgttttgt gctgctccag
    13741 atgtctctgc atcgccgcca cactgtagcg taacatgcga aaaggcatgt acagattttc
    13801 ggtgctctgg tgctcgatga gcgtataaac ataaccatct tcacccccgg tggttttcac
    13861 gctccagagg atatcgctgg cgtactgatg caggtccggc tcaacaaagg tggcgggttc
    13921 cagcttcagc gtggacaggt cgcacaactg ccggtactcc gcaggaagat gcagttccag
    13981 aaaatctctg gcgacgtcgg gattcgccag gaacgaccgg aacgcggcat catgcggcgt
    14041 gggcgttgtg gtgtttttct tcttactcac ggttcctcct ggtgagatgt ctgaacgtat
    14101 cctgccgcct gcacaggcag acggcaaggg ttaatcagtg gcagacccgc tcaatttcaa
    14161 gcgcgcggag caggtggccc cattcgctga ctttcatagc ggggatacgc acccgcttct
    14221 gctcactgca gcgtctggcg atatggccgc tcatcgccag gacgtccggg ttaatctcat
    14281 cctgtgccag ctcctgacgt ctgaagcggg tatggcggtg caggggttta ttctcccgca
    14341 ggcactcttc acgcagacgc tttacatact cggcggttgc gaagataatt gatggtctca
    14401 caatattact ccttatgttg ttgatgactg ccgctcttcc tggcggggaa gtaacggcgg
    14461 agagaggcag ggtttatcgc cctgcctttt tttcatttcg gacagcgacg cagtgccagc
    14521 gcgagcgctt tcatcttcca gccttccggc gtgcggtaca tcaccttcca gtagccgtcg
    14581 atgaacacat aatggaagcg cacctcttca gtgctttcag tggcaatagc ctccctgaaa
    14641 gaacggtact cacgcgcctc actgtccatc cggaaaccac tgtcacgtcc gtaataagcc
    14701 gtcacaccgt ccaccgggtt ctgaagcgta tgcccggcgg gtttgtcaca acggggaccg
    14761 aggcaacgga tatcaccgtg ttcaaccagg gcttcggcct gctgaccggt ggcatagtga
    14821 gtatgcagga tgcgaccgtt ccaggtcggc tccccgtgca ggtggcagta aacggtacgg
    14881 aacaccccgt cgcggcaaaa aacggaaatc gtgctgctga ctgtcatgaa aacatctcct
    14941 tctgctgaat caggtcgccg ggccgaccgg ttgctccccg gaggggcgaa agccccttct
    15001 gccgtccact tcttcggggt ttctccccgc agtggacctt tccgatgggg cgtccgtcac
    15061 cacgtcacgg ggcagctgtc cgggggcgga cgcaaaattt tttgccgggt ttatggctaa
    15121 aaattttgtg gacggtatat cttcccctgg actgctgaaa cgggatgtgg ttactccagc
    15181 ccccggaaag tccacagcgg gaaacaggcc gcaggggtgg cggcggaagg gttgactgcg
    15241 ccatcaggcg cagttgcctt acacaccgta aggtgtgcag ggagcgccgt ttccggcgcg
    15301 acaggggcgg cgcgaccgga acggtcgctg caagcctggt ttttcagaca tcggaccg
//
LOCUS       DAPGRN010000111        15355 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPGRN010000111 DAPGRN010000000
VERSION     DAPGRN010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.113"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..363
                     /locus_tag="RHW33_003712"
     CDS             91..363
                     /locus_tag="RHW33_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV1647172.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(496..1368)
                     /gene="rluF"
                     /locus_tag="RHW33_003713"
     CDS             complement(496..1368)
                     /gene="rluF"
                     /locus_tag="RHW33_003713"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV1647173.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1716..2663
                     /locus_tag="RHW33_003714"
     CDS             1716..2663
                     /locus_tag="RHW33_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDV1647174.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDENEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2733..3539
                     /locus_tag="RHW33_003715"
     CDS             2733..3539
                     /locus_tag="RHW33_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1647175.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3549..3956
                     /locus_tag="RHW33_003716"
     CDS             3549..3956
                     /locus_tag="RHW33_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HDV1647176.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQSCVVWRQVATAEEDF"
     gene            3956..4450
                     /locus_tag="RHW33_003717"
     CDS             3956..4450
                     /locus_tag="RHW33_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV1647177.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVANIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4516..5313
                     /locus_tag="RHW33_003718"
     CDS             4516..5313
                     /locus_tag="RHW33_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDV1647178.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5324..6148
                     /locus_tag="RHW33_003719"
     CDS             5324..6148
                     /locus_tag="RHW33_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV1647179.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6200..7429
                     /gene="sorE"
                     /locus_tag="RHW33_003720"
     CDS             6200..7429
                     /gene="sorE"
                     /locus_tag="RHW33_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HDV1647180.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYEGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILTRHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7810..8499
                     /gene="pepE"
                     /locus_tag="RHW33_003721"
     CDS             7810..8499
                     /gene="pepE"
                     /locus_tag="RHW33_003721"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV1647181.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8589..10220)
                     /locus_tag="RHW33_003722"
     CDS             complement(8589..10220)
                     /locus_tag="RHW33_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV1647182.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10440..14123)
                     /gene="metH"
                     /locus_tag="RHW33_003723"
     CDS             complement(10440..14123)
                     /gene="metH"
                     /locus_tag="RHW33_003723"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV1647183.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLSLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIDEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQAERPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAREVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14323..15147
                     /gene="iclR"
                     /locus_tag="RHW33_003724"
     CDS             14323..15147
                     /gene="iclR"
                     /locus_tag="RHW33_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV1647184.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3640 a         4053 c         4032 g         3630 t
ORIGIN      
        1 tatttaagaa gagaacaaag gaatattatc agttctgctc gcaaacgaat tcccgtacac
       61 tgaactctct ggctcactga gggcaccatc atggcactcc cccgtattac ccaaaaagag
      121 atgactgagc gcgagcagcg tgaacttaaa acactgctgg atcgggcgcg gattgcgcat
      181 ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga aagagtacat cgataaatta
      241 atggtcgagc gtgaggctga agcgaaaaaa gcccgccagt taaagaaaaa gcaggcttat
      301 aaaccggatc ctgaagcgtc atttagctgg tcagcaaata cgtcgacgcg cggcaggcgc
      361 taatgaacaa tcaacgcctg agattttcac tcgtctgtgt ggacacaatc ttcccctcaa
      421 cctaaccctc tccccgaagg ggcgagggga ctgtccgagc actttttgga cgttttcatc
      481 agtctgaggc gttaatcagc gtcccttctt tttacgcccc ggcgaggtaa aacgcttacc
      541 gttggacgcc gggcgaccgc ctttttccgc cgttttttcc atcttaacga ctggacgttt
      601 gatgcccgcc gttttcggtt tagccttcgc tttaggtttt gcctcggaag aggaattttc
      661 aatgagctta aagaggtcga ttaactcatc gtcggttaaa tcgcgccact cccccagtgg
      721 aatgccgctt aagctgacgt tcataatgcg cgtacgttcc agctttttca cttcgtagcc
      781 aaagtgttcg cacatgcgac ggatctgacg gttcagtccc tgcactaacg taatgcggaa
      841 gacaaacggc gcttcttttt tgactttgca ctttttagtc accgtcccga ggatcggcac
      901 ccccgccccc ataccacgaa taaattcatc ggtaatcggt ttatcgaccg tcaccagata
      961 ctctttctca tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt
     1021 gaggaaaatc agcccctggg agtctttatc caggcggcca attgggaaca cgcgtttgct
     1081 gtggttaacg aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc
     1141 gacgggcttg ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg
     1201 accgtttact ttcacaacgt cgccgggttt cacctgatcg ccaatggtgg ctcgcttgcc
     1261 attaaggaac acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc
     1321 gctttcgctg atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt
     1381 aaaacgcgga atataccgta cctcttagcg gggaaaaaca gggttatgcg gcaacgtcga
     1441 aattaaatgt gatctgccac gccttttatc acaaaagtgt ctccggaagt acaatgcaaa
     1501 tttgcctggc aaaataagga taatcaacat attacgaatc gctgcacacc tgtgctgatg
     1561 actactgcgc agagaaacaa atgtgcaaga gaaagcgcta tttctcccca tgcaaagagt
     1621 gaaaaatcgc tttgaaaggc catcatgatg gggttatatc agatcaagcg gtgagaaagc
     1681 gtatttgcac aaatgtgcag aaccaggagg cggcaatgga aaacagtgac gatatccgtt
     1741 tgattgtgaa gattgcccaa ctctattacg aacaggatat gacgcaggcg caaatcgcgc
     1801 gcgaactggg tatttaccgc accaccatca gccgcttgct taaacgaggc cgcgatcagg
     1861 gaattgtcac catcgccatc aactatgact acaacgaaaa tctctggctg gagcagcaac
     1921 tgaagcaaaa gtttggcctg aaagacgttg tggtggtgtc gggaaatgat gagaatgaag
     1981 agactcaact ggcgatgatg gggttacacg gcgcacaact gctggatcgc ttgctggagc
     2041 ctggcgatat tgtcggtttt tcctggggtc gcgcggtgag cgcactggtt gaaaacttgc
     2101 cgcaggcggg gcaatcgcgg cagttaatct gcgtaccaat tattggcgga ccgtccggta
     2161 aactcgaaag ccgctatcac gtaaacacat taacctacag cgcggctgcg aagctgaaag
     2221 gggaatcgca tctcgcggat tttccggctc tgctggataa ccccttaatt cgtaatggga
     2281 tcatgcagtc tcagcacttt aaaaccatct ctgcctactg ggataatctg gatgtcgccc
     2341 tggtgggaat tggctcaccg gccattcgcg acggcgctaa ctggcatgcg ttttatggtg
     2401 gtgaagagag tgacgacctg aatgcccgcc aggttgctgg cgatatttgc tcgcgctttt
     2461 ttgatattca cggcgcaatg gttgaaacga atatgagcga aaaaacactc tctatcgaaa
     2521 tgaataaatt aaagcaggca cgatattcca ttggcattgc catgagtgaa gaaaaataca
     2581 gcggaattat tggtgcactg cgtggaaaat atattaattg tctggtaacg aatagcagca
     2641 cagctgaact attactgaaa taaaaatctt cccgatgttt atcgtaataa aaattacggc
     2701 gggtccctat tatctaaaat caggagtctg ttatgcaaac gtggttaaat ttgcaggata
     2761 aaatcattat cgtcaccggc ggcgcatccg gtattggtct ggcgattgta gaggaattat
     2821 tagcacaagg cgcaaacgta cagatggtag atattcacgg tggcgatggt caatatgaag
     2881 ttcataaagg ctatcagttt tggccgaccg atatttccag cgccaaagag gtaaatcata
     2941 cggtagcgga aattatccag cgttttggtc gcatcgacgg tctggtcaat aacgccgggg
     3001 tcaatttccc gcgtctgctg gtcgatgaga aagcgcctgc cgggcagtat gaactcaacg
     3061 aagctgcatt cgaaaaaatg gtcaatatca accagaaagg cgtttttctg atgtcgcagg
     3121 cggtggcgcg acagatggtc aaacaacatg atggcgtgat tgtgaatgtt tcctcagaaa
     3181 gtgggctgga aggctcagaa ggacaaagct gttacgccgc taccaaagcc gcgcttaata
     3241 gcttcacgcg atcctggtcg aaagaattgg gtaaacacgg tatccgtgta gtcggtatcg
     3301 cgccggggat tctggaaaaa accgggctac gcacgccgga atatgaagaa gcgctggcgt
     3361 ggacgcgcaa tatcaccgtc gagcagctgc gtgaaggcta taccaaaaac gccattccta
     3421 ttgggcgcgc cggaagatta gcagaagtcg ctgattttgt ttgttatctg ttgtctgaac
     3481 gcgccagcta catcaccgga gtaaccacta acattgcggg cggcaaaacg cgcggataag
     3541 gaggctttat ggtcaacgcc attttttgtg cccacggcaa attagcttgc gccatgctgg
     3601 aatcggttca gatggtttac ggcaacgcca atgttgaagc cgttgaattt gtgcccggcg
     3661 agaacgccgg agacatcgta gcaaagctgg aaaagttagt aagcattcac aatcaagatg
     3721 agtggctgat tgccgtggat ttacagtgcg gcagcccgtg gaatgcggca gcgatgcttg
     3781 cgatgcgcaa tcctcgtctg cgggtaatca gcggcctttc tctgccgctg gccctcgaac
     3841 tggttgataa ccaggacagc atgaatgttg atgaactgtg tgaacacctg acgcaaatcg
     3901 caaagcaatc ctgcgtcgtc tggagacagg tggcaacggc agaggaggat ttctgatgaa
     3961 cattacgctc gcccgcattg atgaccgcct gatccacggt caggtcacta ccgtctggtc
     4021 gaaagtggct aacgcccagc gcattattat ctgcaatgac gaagtttata acgatgaagt
     4081 tcgccggaca ttattgcgtc aggctgcgcc accgggaatg aaggttaacg tggccaatat
     4141 tgaaaaagcc gtcgcggttt atcacaaccc gcaatatcag gacgaaaccg ttttttatct
     4201 tttcactcgc ccacaggatg ccttagcgat ggttcgccag ggggtaaaaa tagacaccct
     4261 gaatattggc ggcatggcct ggcggcccgg taaaaaacag ttaaccaaag cggtttcatt
     4321 agatgatgat gatattaacg catttcatga gttaaataat cttggcgtca tactggattt
     4381 acgcgtcgta gcgtcagatc cctcaatcaa tattatcgat aaaataaacg aacagttaat
     4441 cgcaaattaa ataatagcgc ctgtgtacat atgctttaac aggcagggat gacgacactt
     4501 aaaaggtgac aaatcatgga aataagtacc ctacaaatta ttgctatatt tcttttttcc
     4561 tgtattgccg gaatgggcag cgtgctggat gaattccaga ctcatcgccc gttaattgcc
     4621 tgtacggtga ttggtttaat tctcggtgat ttaaaaaccg gaataatact cggtggtacg
     4681 ctggaattga tagctctcgg ctggatgaac gtcggcgcgg cgcaatctcc ggactctgca
     4741 ctcgccagca taatctccgc cattctggtt atcgttggtc agcaaagcat cgccaccgga
     4801 atcgccatcg cgttgcctgt ggctgcggca ggccaggtgc tgacggtgtt tgcccgtacc
     4861 atcaccgtag tgttccagca cgcggcggat aaagcagcgg aagaggcgcg gtttcgcact
     4921 ctcgatattc tgcatgtctc cgcgcttggc gtgcaggcgc tgcgcgttgc tattccggca
     4981 ctgattgtct cgctgttcgt cagcgccgat atggtcagca atatgttaaa cgccattccc
     5041 gaatttgtga cccgaggact acagattgct ggcggtttta tcgtggtggt cggttacgcc
     5101 atggtgctac gtatgatggg cgtgaaatat ttgatgcctt tctttttcct cggtttcctc
     5161 gcaggtggct acctcgatct cagtctgctg gcgttcggtg gcgtcggcgt gatcatggcc
     5221 ctggtctaca tccagctaaa tccacagtgg cgtaaagctg aacctcaact ccaggccacc
     5281 gcctccaccg cccttgacca acttgacgat taacggagcc atcatggaac agagaaaaat
     5341 tacacgcagc gatctggtga gcatgtttct gcgctccaac ctgcaacagg cgtcctttaa
     5401 ctttgaacgt attcacgggc tgggtttttg ctacgacatg atccccgcca tcaagcgact
     5461 ttacccatta aaagaggatc aggttgcggc gctcaggcga cacctggtgt tcttcaatac
     5521 cacgccagcc gtatgtggcc cggtcatcgg cgtcactgcc gccatggaag aggcgcgggc
     5581 caacggcgcg gaaattgatg acggtaccat taacggcatc aaagtcggtc tgatgggacc
     5641 attggcaggg gttggcgatc cactggtctg gggaacgctg cgcccgatta ccgccgcgct
     5701 cggcgcatct ctggcacttt cgggcaacat tctcggcccg ctgctgttct tctttatttt
     5761 caacgcggtg cgtctggcga tgaagtggta tggcctacag ctcggctttc gcaaaggggt
     5821 gaatatcgtc agcgatatgg gcgggaatgt gctgcaaaaa ctcaccgaag gcgcgtcgat
     5881 tctcggactg tttgtgatgg gcgtgctggt caccaaatgg acgtcaatca acgtaccgtt
     5941 ggtggtttca caaacgcatg ccgccgatgg ctccaccgtc accatgaccg tgcagaacat
     6001 tctcgaccaa ctttgccctg gtttgctggc gctcggtctg acgctactaa tggttcgtct
     6061 gctcaacaaa aaaattaacc cggtatggct gattttcgcc ctgtttggct tagggattat
     6121 cggcaatgcg ctgggcttcc tgtcctgatt cttcgccccg gcacgactgc cggggccatc
     6181 gctcaacatg aggtggttta tgaaaacaac agctctgcgt ctttatggta aacgtgattt
     6241 acgcctggaa acctttgacc ttcctgaaat gcaggaggat gaaatcctcg cgacggtggt
     6301 cactgacagc ctgtgcctct cttcctggaa agaggccaac ctgggtgaaa accataaaaa
     6361 agtacccgac gatgtggcga ccaacccaat catcatcggc cacgagtttt gcggcgatat
     6421 tctggccgtg ggtaaaaagt ggcagcacaa attccagccg ggtcagcgtt atgtaattca
     6481 ggccaacctg caactccccg accgcccgga ctgccccggc tactccttcc cgtgggtggg
     6541 tggtgaagcc acacatgtgg ttatcccaaa tgaggttatg gaacaagatt gcctgctggc
     6601 atacgaaggc gaaacctact ttgaaggctc gctggttgaa ccgctttcct gcgtgattgg
     6661 cgcgttcaac gccaactatc atcttcagga aggtagttat aaccacacga tggggattcg
     6721 cccgcaaggg cgcacgctga tcctcggcgg caccggacca atgggactgt tggcgattga
     6781 ttatgcgcta catggacccg ttaacccgtc gctgctcgtc attaccgata ccgacaacga
     6841 taaattgagt tatgcgcgca agcactatcc atcagaaccg caaacactga ttcattatct
     6901 caatgccacc gatgcagcat ttgatacgct aatggcgctg agtggcggtc acggcttcga
     6961 tgatattttc gtctttgtgc ctaatgaagg actggtgact ctcgcctctt ccttgctggc
     7021 gacagatggt tgcctgaatt tcttcgccgg accgcaggat aaacatttca gcgcgccaat
     7081 taatttctac gatgtgcatt atgcatttac ccactacgtg ggcacgtcag gcggcaatac
     7141 cgacgacatg cgcgcagcgg tcaaattgat tgaagagaaa aaagtgcagg ccgcaaaagt
     7201 ggtaacacat attcttgggc tgaatgccgc gggcgaaacc acgcttgaat tacctgccgt
     7261 cggcggcggc aaaaagctgg tgtataccgg gaaatacctg ccgctgacgt cactcacgca
     7321 gattcaggat caagcactgg cggcgattct gacgcgtcat caggggatct ggtccggtga
     7381 ggcggagcaa tatctgctca ctcatgcaga ggcaatttcc catgattaat cgcgttactc
     7441 aactctgcat gtcgctggct ggtcgcacca gcaacttcgc gaccggatgt gctgctgatt
     7501 gctgaagcag cacaatttgc acgagaagca taaaaaatat cagcgcctta aggggcgctc
     7561 aaattggtgg caaagtgcac attgtttatg ccggatgcgg aatgaagcct tattcggtct
     7621 acataatttt gaaaattcaa tatattgcag ttatctggta ggccaggtaa gcgtagcgca
     7681 tcaggcaatg ctgtgtttgt catcagtctc agcgcctgtg caatacgtga catcgtgagc
     7741 catgtcagta agtggttaaa ttcgcagtgg tagcattcct tccgtgattt cttactaacc
     7801 ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg
     7861 gaacatgcac tgccgctaat tgctgaacag ttgcagggtc gccgctcagc ggtgtttatc
     7921 cctttcgctg gcgtaacgca gacctgggat gattacacag agaaaacggc tgcggttctc
     7981 gctccgctgg gtgtttctgt caccggtatt catagcgttg tcgatcccgt tgccgcgatt
     8041 gaaaatgctg agatcgtgat tgtcggcggc ggtaatactt tccagttgct gaaacagtgc
     8101 cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggcgc actgtatatt
     8161 ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg
     8221 attgtcgatc cgcaaggttt cgatgcgctg aatctgttcc cgttgcaaat caacccgcac
     8281 ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgaacagcg tattcgcgaa
     8341 ctgctggtcg tcgcgccaga actgacgatt attggtctac cggaaggtaa ctggatcaca
     8401 gtgagtaaag gtcacgcaac gctgggtggc ccgaacacca cctatgtgtt taaggccggt
     8461 gaagaagcgg ttccgctgga agcgggtcac cgtttttaag tgaattgccg gatgcatgat
     8521 ccggcatctt gttcatagtg cctgatgcga cgctttacgc gtcttatcag gcctacaggg
     8581 tcacggtatt aatactcgtc ccgtccttca tcttcatccg gctgttccag cacactgtaa
     8641 gccaccgaac aaaacagcga gttcagacgc tgcatatctc ccagtaagcc taaatgtagc
     8701 gaactggttt caatgctctg cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa
     8761 tagcggcgat taagaatgcg aaagcgatgc ttgctgcggc gcaaacgacg ggcgctggta
     8821 acatcaccag agaagaaaac cgacattgcc agttttaaat tactgagcaa ttgctcatag
     8881 agcgcatcca gttccttcaa gccatcaagc gaaaatgccc gccgtgctgc cagcgattta
     8941 tcggcaattt cgctccccat gcgctcgacg atatcggagg cctgttcaag gttgagcgac
     9001 atttcgatga tctccgccca gcggcgcgac tcttcctccg ccagctcttc ttttggcatc
     9061 cgcgccagat acagcttaat ggcggtatag agaacgttga tatcatctgc cagcttacgc
     9121 agctctttct cctgccgtgg ctcgccgtgc atcactttat tcagcccttc catcatctgt
     9181 tccatggcgt cgccaatgcg cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc
     9241 gtatccagcg cgctgacatc cagatgtttg ggccgtagct gggtatccag ttccggttca
     9301 tcgcgaataa tcgttttgca aaaccgtgcc atcgggtcaa caaatggcag catgaccagg
     9361 caacgtacaa ggttgtagaa gacgtggaaa tagatcacca gttccgcttt tggcaatgac
     9421 aacttcccca ttgtctctgc cagcaaatgg acaaacggca ggataatcag gctacccacc
     9481 agcttaaaca gcaaactacc cagcgcgaca cggcgggctg cggcattggc ggcactgttg
     9541 ttgagcatcg ccagcaggcc ggaaccgagg ttagcaccaa tcaccagaca gagcgccacg
     9601 gggaaggaga taatgcctgc ggcggtcaga gtcgcagtca gcagtacagc agcaaggctg
     9661 gagtagctga taatggcgaa catcgcgcca atcagcgcat ccagcagaat atcgccggtc
     9721 agcgaggcaa agatcacctg aacgccgttt gcctgggtga tcggcgttac ggcctgcaca
     9781 atcaactcca gcgccagtaa aatcagccca agaccaatac cgacgcggcc cagttgcccg
     9841 gcgcgtgact gtttgcgtcc gaggaaaaaa atcacgccga taaaaataag taacggtgac
     9901 agccaggata agtcgaaggt gagaatacgc gccattagcg ccgtcccgac atctgccccc
     9961 agcacaatga ccagagccgg tgcgagggct accagatcct gagcgacgaa cgaggtcacc
    10021 agcatggtgg tggcattact gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag
    10081 gcgagcggct ttttttcgac gctccggcta aggacagtac gcaaacgcgc gccgaagacg
    10141 cgcattacgc cggttcgaac aatatgagtc ccccagacca gcagggcgac ggcagaaagc
    10201 aggtgaagca gcgttaacac gagttcaggt tctccttatc gttatatgtt cctgaagcaa
    10261 ttgaatgacc aaaggaccgg aaacacgctg acgggcatct ttactgtgcc ttttgacatt
    10321 cgtttttccg gctaagatta ttccagcctg tttccagtat aagtgtttca tattaataaa
    10381 gagacagggc actcattgag cgccctgttt gttgtaagga aaagtgacag atttgtgaat
    10441 cagtccgcgt cataccccag attcggtgcc agccagcgct caacatcgga gacgctcata
    10501 cctttacggc gggcatagtc ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac
    10561 ttgctgtccg ggtggctgaa ataccaaccc gaaaccgatg caccgggcca catggcgaaa
    10621 gattctgtga gtttcatgcc ggtatgtttt tccacttcca gcagctccca gatggtggct
    10681 ttttccgtat gttccgggca ggccgggtag cccggtgccg gacggatgcc ctggtagttt
    10741 tcgcggatca gttcttcgtt gcttaggttc tcgttcggcg catagcccca gtagacttta
    10801 cgcacacgct catggagata ctccgcaaag gcttcggcta aacggtcggc aagcgctttc
    10861 accatgattt tgttgtaatc atcgtgctgc gcttcaaagg catcagccag tgcgtcctct
    10921 tccagcccgc cagtcacggc aaatgcgccg atgtaatctg ctttaccaga aagcttcggc
    10981 gcaacgaagt cagcgagaca gtagttagcg aatcccgttt tttcggtctg ttgacgcaga
    11041 tggtggctga cgttgatcac atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg
    11101 cccacacggt ttgccgggaa cagccccacc acaccacgcg gattcagcgt tttctcggcg
    11161 cttaatttat ccagcatgtc gttggcgtct ttaaacagcc gctgcgcctc aacgcctacc
    11221 acttcatctt ccagaatgcg cggatacttc ccggccagcg accaggtcat aaagaacggc
    11281 gtccagtcga tgtaattacg cagcgtttcg atgctggctt cgacttcctg cacgccgaga
    11341 cggtgcgcca ccggcggcgt gtaagcctgc cagtcaaaag cgaagtcgtt atcgcgcgcc
    11401 gcttccagcg tgaccggtgg tgtgcgcggt ttcttgcgcc cgtgctgaat acgtacggtt
    11461 tcgtactcct tgcgggtacg agcgacaaaa tcatcgcgct gagtgtcaga aagcagcgcc
    11521 gccaccacgc caacggtgcg cgaggcattc tgcacataca ccgtcgggcc gctgtagttc
    11581 tgctcaattt tcaccgccgt gtgcgctttt gaggtcgtcg cgccaccgat cagtaacgga
    11641 atggtgaagc cctgacgctc catctctttc gccacgttaa ccatctcgtc cagcgacggc
    11701 gtgataagcc ccgagaggcc aatcagatca gcattcactt ctctggcggt gcggaggatt
    11761 ttttccgcag gcaccataac gccgagatcg acaatttcgt agttgttaca ttgcagcacc
    11821 acaccaacga tatttttacc gatgtcgtgg acgtcgccct tcacggtggc gatcaccatc
    11881 ttgccgttgg ttttaccctg ctctttgctg gcttcaataa acggttcgag atacgccacc
    11941 gcctgcttca ttacgcgcgc cgatttcacc acctgcggca ggaacatttt gccctcgcca
    12001 aacagatcgc cgaccacgtt catgccgtcc atcagcggac cttcaatcac ttcaatgggg
    12061 cgctctgcct gctgacgggc ttcttcggta tcctgctcga taaactcggt aatgcctttg
    12121 accagcgagt actccagacg tttattcacg tcccacgagc gccactccgc ctgctgggcg
    12181 ttagcggtgt cgtccgcttt actgccgcga tatttctcgg caagttccag taaacgctcg
    12241 gtaccatcgt cgcgacgatt gaggatcaca tcttccaccg cgtcgcgcag ttcagcggga
    12301 aggtcgtcgt aaatcgccag ttgcccggcg ttgacgatcc ccatatccat gccattacga
    12361 atagcgtagt agaggaacac cgcgtgaatg gcttcgcgca ccggatcgtt accacggaac
    12421 gagaacgaga cgttggagac gccgccggag atcagcgcgt gcggcagttc gcgtttgatg
    12481 tcttcacacg cgccgataaa gtcctgcgcg tagttgttgt gctcgtcaat gccggtggcg
    12541 accgcgaaga tgtttgggtc gaagatgata tcttctggcg ggaagccaac ctcttcggtg
    12601 aggattttgt acgcccgacg gcaaatctcg attttccgcg cgcgggtatc ggcctggccc
    12661 tgttcgtcga aggccatcac cactactgcc gcaccatagc gacgcaacag cttcgcgtga
    12721 tggataaagg cgtcgacgcc ctctttcatc gagatagagt taacaatgcc tttgccctgg
    12781 atacacttca gacctttttc aatgacgtcc cattttgagg agtcgatcat aatcggcacg
    12841 cgagcgatat ccggttcacc ggcaatcaag ctgaggaaac gcaccatcgc cgcttcggca
    12901 tctagcatcc cctcatccat gttgatatcg ataatctgcg cgccgttttc cacctgttga
    12961 cgcgcgacat ccagcgcctc gctgtatttc tcttctttga tcagacgctt gaacttagcg
    13021 gaaccggtga cgttagtccg ctcgccgacg ttcacaaaca ggctgtcgtc gccaatgttc
    13081 agcggctcca ggccggacaa acggcaggct acgggaattt ccggcagttt gcgcggcgct
    13141 aaaccttcta ctgcacggct catcgctgca atatgttgtg gcgtggtgcc gcagcagccg
    13201 ccgacgatat tgagaaagcc cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg
    13261 gcgtcgagat cgtactcacc aaaggcgttg ggcagcccgg cgttcgggtg ggcggtgacg
    13321 tagcattccg caatccgtga tagttcctgc acgtactggc gcagttcgtc tggccccagc
    13381 gcgcagttga ggccaaaagt cagggcttcg gcatggcgca gggagttgta aaatgcttcg
    13441 gtggtctgtc cggagagcgt gcgcccggag gcgtcggtga tggtgccgga gatcataatc
    13501 ggcagctcaa cgcctaacgc ttcgaactcc gttttcaccg caaagaccgc cgctttggcg
    13561 ttaagggtgt cgaaaacggt ttcaatcagg atcagatccg caccaccttc caccagcgct
    13621 ttggtagatt cgcgataggc cgccaccaac tggtcaaaag tgatgttgcg gaatgccgga
    13681 tcgttcacgt ccggagatat agacgccgtg cggttggttg ggccgagaac gccggcaacg
    13741 tagcgcggtt tctccggcgt gcgggcggtc cattcatcgg cacaggcacg cgccagtttc
    13801 gccgccgcaa agttgatttc cgccgacagg gattccatct ggtaatccgc catcgcaatg
    13861 gtcgtggagt tgaaggtgtt ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg
    13921 tggatagcgg cgatcacttc cggtttactg agcaccagca ggtcgttgtt gcctttgaga
    13981 tcgcacggcc agtcggcaaa gcgttcacca cgaaaatcgg cttcgttcag acgatagctc
    14041 tggatcatgg tgcccatacc gccgtccagc accagaatac gttcatttaa ctgcgcacgc
    14101 agttgttcca ctttgctgct cacactcgct cccgacacgc tcaacccaga tttaataaaa
    14161 attcaacaaa ccatactggc ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg
    14221 agatttgttc aacattaact catcggatca gttcagcaac tattgcatta gctaacaata
    14281 aaaatgaaaa tgatttccac gatacagaaa aaggagactg tcatggtcgc acccattccc
    14341 gcgaaacgcg gcagaaaacc cgccgttgcc accgcaccag cgactggaca ggttcagtct
    14401 ttaacgcgtg gcctgaaatt actggagtgg attgccgaat ccaatggcag tgtggcactc
    14461 acggaactgg cgcaacaagc cgggttaccc aattccacga cccaccgcct gctaaccacg
    14521 atgcaacagc agggtttcgt gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca
    14581 catgccttta tggtcggcag cagctttctc cagagccgta atttgttagc aattgttcac
    14641 cctatcctgc gcaatttaat ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat
    14701 caaagcgatc acgaagcgat tattatcgac caggtacagt gtacgcatct gatgcgaatg
    14761 tccgcgccta tcggcggtaa attgccgatg cacgcttccg gtgcgggtaa agccttttta
    14821 gcccaactga gcgaagaaca ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat
    14881 acccacgcaa cgctggtgtc tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa
    14941 cggggttatt catttgacga tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt
    15001 attttcgatg agcaccgtga accgtttgcc gcaatttcta tttccggacc gatttcacgt
    15061 attaccgatg accgcgtgac cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg
    15121 acgctggcgt acggtggaat gcgctgactt tttctggcgg gcagaggcaa tattctgccc
    15181 gtcatacctg agtggtaata gaataagggt gtctgttaat cgcattgacg ccaaaataac
    15241 ttattgtcat acacttcaca cacttttatt aactcagcat tatttttaaa catcaaacca
    15301 cttaattatt aacaaaatcg caagatctca ttccaccgaa acttttacgg ctgca
//
LOCUS       DAPGRN010000112        15320 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPGRN010000112 DAPGRN010000000
VERSION     DAPGRN010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.114"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..616
                     /locus_tag="RHW33_003725"
     CDS             50..616
                     /locus_tag="RHW33_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV1647185.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            620..1195
                     /locus_tag="RHW33_003726"
     CDS             620..1195
                     /locus_tag="RHW33_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV1647186.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1237..2289)
                     /locus_tag="RHW33_003727"
     CDS             complement(1237..2289)
                     /locus_tag="RHW33_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV1647187.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALDGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARRAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2514..3434
                     /gene="lpxL"
                     /locus_tag="RHW33_003728"
     CDS             2514..3434
                     /gene="lpxL"
                     /locus_tag="RHW33_003728"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV1647188.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3606..4832
                     /gene="mdtG"
                     /locus_tag="RHW33_003729"
     CDS             3606..4832
                     /gene="mdtG"
                     /locus_tag="RHW33_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV1647189.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4915..5289
                     /locus_tag="RHW33_003730"
     CDS             4915..5289
                     /locus_tag="RHW33_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV1647190.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5290..5517)
                     /locus_tag="RHW33_003731"
     CDS             complement(5290..5517)
                     /locus_tag="RHW33_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV1647191.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="RHW33_003732"
     CDS             complement(5690..8233)
                     /gene="mdoH"
                     /locus_tag="RHW33_003732"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV1647192.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWERED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="RHW33_003733"
     CDS             complement(8226..9761)
                     /gene="mdoG"
                     /locus_tag="RHW33_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV1647193.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10155..11312
                     /gene="mdoC"
                     /locus_tag="RHW33_003734"
     CDS             10155..11312
                     /gene="mdoC"
                     /locus_tag="RHW33_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HDV1647194.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRT
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11320..12801)
                     /gene="clsC"
                     /locus_tag="RHW33_003735"
     CDS             complement(11320..12801)
                     /gene="clsC"
                     /locus_tag="RHW33_003735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV1647195.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMAHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="RHW33_003736"
     CDS             complement(12743..13276)
                     /gene="ymdB"
                     /locus_tag="RHW33_003736"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV1647196.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13371..13682)
                     /locus_tag="RHW33_003737"
     CDS             complement(13371..13682)
                     /locus_tag="RHW33_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1647197.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="RHW33_003738"
     CDS             complement(13803..14135)
                     /gene="csgC"
                     /locus_tag="RHW33_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV1647198.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
     gene            complement(14194..14649)
                     /gene="csgA"
                     /locus_tag="RHW33_003739"
     CDS             complement(14194..14649)
                     /gene="csgA"
                     /locus_tag="RHW33_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV1647199.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQIGFGNNATAHQY"
     gene            complement(14690..15145)
                     /gene="csgB"
                     /locus_tag="RHW33_003740"
     CDS             complement(14690..15145)
                     /gene="csgB"
                     /locus_tag="RHW33_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV1647200.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3810 a         3940 c         3653 g         3917 t
ORIGIN      
        1 ctccgacacg ccattttctt tctctcgcaa attcgttttg gatgcactta tgtcattcac
       61 aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat
      121 tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt acgatggctg
      181 gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt
      241 tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc
      301 catgactcgt cttgccgcaa aagctggaca tctcgcattg tacctattgc tgttcgccat
      361 cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg
      421 gtttgacgtc cccgcgactc ttgccgacgc tggcgcacag gcagactttg ccggtgccct
      481 gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat ttatggccct
      541 gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc
      601 tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat
      661 gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc
      721 ctttgttaac ttccgcatcc agcatcttgg ctatagctgg ttatatggca cctttaaaga
      781 tttcgacggt acttttacct ttgatgaaaa aaatccagct gccgataaag tgaatgtgac
      841 aattaacacc accagcgtcg atactaacca cgccgaacgc gataaacatc ttcgcagtgc
      901 ggattttctc aataccgcaa aatatccaca ggcaacattc acctccacca gcgtgaagaa
      961 agacggtgac gaactggata ttaccggcga tctgacgctg aatggtgtaa ccaaacccgt
     1021 cacgctggaa gcgaaattaa ttggtcaggg cgacgacccg tggggtggta aacgagcagg
     1081 ctttgaggcc gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag atttaggtcc
     1141 agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagagtg
     1201 aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat caggaatgcc
     1261 cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc
     1321 tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact
     1381 acaacaacct ttgtattttt ccgcacatac tggacactga ataaacagca ggtggcagcc
     1441 atcattttta cagttggtat ggctgtcgca cggcgcaccg cactggtggc aatgcgcaat
     1501 aatctcatca gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa
     1561 acgcaccggc aagccctgct cgcgcgccct acgggcgtat tcaataattc cgccctcgat
     1621 atgccagact ttattgaatc cgttatgttt catccaggca ctggcctttt cacaacgaat
     1681 gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc
     1741 ttttggcagc tgctcacgga aagtatctgc cggaatttcc agcgcgtttt caaagtgccc
     1801 cacttcatac tcatagtggt tacgcatgtc gataaacagt gcatcgggat cgtcaagcat
     1861 ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa agtgaggatc
     1921 gtcaataccg tcggcaacga tacgatcgcg gaccttcatg cgcagtaccc agaaggattt
     1981 cccgtcatca tccaacgcga tattcaggcg taagccatct aaagccgggt cgaaggcata
     2041 gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg cgttaatgcc
     2101 ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct gatataaagc
     2161 gtcacgggtc gccttaggat cggcgatgtg gaaatactta taaaacgaaa tggtggttcg
     2221 cggttcgctc tcagccaaca ttttggcttt taacgcgtcg ttggaaatgc ggttgtgtaa
     2281 cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg catgatatag
     2341 caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta aaaattattt
     2401 gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg ttgcgggcga
     2461 aaaaatgcga caatacatac aattgcccga ataggttgaa aaacaggatt gatatgacga
     2521 atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc tggttgggta
     2581 ttggcgtact ttggttagtc gtgcaattgc cctacccggt tatctaccgc ctcggttgtg
     2641 gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg catcgcaacc
     2701 tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt
     2761 tcgaatccgt tggcatggga ctgatggaaa ctggcatggc gtggttctgg ccggaccgcc
     2821 gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa
     2881 aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggt gcgcggcagt
     2941 ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact
     3001 ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa
     3061 aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg gatcatgatt
     3121 acggcccgcg ctcaagcgtt ttcgtcccgt tgtttgccgt tgagcaggct gcgaccacga
     3181 ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc
     3241 gtaagccaga tggcaaaggg tatcaattga ttatgctgcc gccagagtgt tctccgccgc
     3301 tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa tgcatcatga
     3361 tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg gaaggcgttc
     3421 cttcacgcta ttaaatcttc catgccggat gcttcagaat ggcatccggc attaccacag
     3481 caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga agtcgggcgc
     3541 ataattagtg tgcttatctt ttcttcttat gttcaccgcg cctggcgcac caacagcgga
     3601 ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac ctgatcgtcg
     3661 cctggctagg ctgttttctt accggtgccg ccttcagtct ggtaatgccc ttcttacccc
     3721 tctacgttga gcagcttggc gttaccggcc actccgccct gaatatgtgg tccggtattg
     3781 tcttcagcat tacattttta ttttcggcca tcgcctcacc gttttggggt ggactcgccg
     3841 accgtaaagg ccgaaaactc atgctattac gctctgctct cggcatgggc atcgtgatgg
     3901 tgttgatggg gctggcacaa aatatctggc agtttttgat cctgcgggcg cttcttggtt
     3961 tacttggcgg atttgtcccc aacgctaatg ctcttatcgc cacacaagta ccgcgtaata
     4021 aaagcggctg ggcgctgggt acgctctcca caggcggcgt tagcggcgcg ttgctcggcc
     4081 caatggctgg cggcctgctc gccgatagct acggcttacg tccggtattc tttattaccg
     4141 ccagtgtgct catactctgc tttttcgtca ccctgttctg catcagagaa aaattccagc
     4201 cggtcagcaa aaaagagatg ctgcacatgc gggaagtggt gacatcactt aaaaacccga
     4261 aactggtact cagcctgttt gtcactacgt taatcatcca ggtggcgacg ggctcaatag
     4321 cccccattct gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac gtcgccttta
     4381 tcagtggcat gatcgcctcg gtgccaggcg tggcggctct gctgagtgca ccacgactcg
     4441 gcaaacttgg cgatcgaatc ggacccgaaa agatcctgat tacggcgctg atcttttctg
     4501 tactgctgtt gatcccaatg tcatacgttc agacgccatt acaacttggg attttacgtt
     4561 ttttgctcgg tgccgccgat ggtgcactac tccccgccgt acagacactg ttggtttaca
     4621 actcgagcaa ccaaatcgcc ggacgtatct tcagctataa ccaatcgttt cgtgatattg
     4681 gcaacgttac cggaccattg atgggagcag cgatttcagc gaactacggt ttcagagcgg
     4741 tatttctcgt caccgctggc gtagtgttat tcaacgcagt ctattcatgg aacagtctac
     4801 gtcgtcgtcg aataccccag gtatcgaact gatttttcgc ctttcatact tgcaaaagcg
     4861 gagaatcagc tatccttttc cctgaaacct catcaactca aagggagaat cgtgatgacc
     4921 atgtacgcaa cgcttgaaga agccattgac gctgcacgcg aagaatttct tgcagacaac
     4981 cccggcatcg acgccgaaga tgcgaatgtg caacagttca atgcccaaaa atacgttttg
     5041 caggacggcg acatcatgtg gcaagttgag ttttttgccg acgaagggga agaaggtgaa
     5101 tgtttaccta tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga ctatgatgag
     5161 atagagatcc gccaggagtg gcaagaagag aatacattac atgaatggga cgagggggag
     5221 tttcagcttg agccaccgct ggataccgag gaaggacgcg cagcagctga tgagtgggat
     5281 gaacgttaat cactcatacg ggccatgatg aatgtcgatc ggcagcagta aagtatcaaa
     5341 aaccagggag aatggcagat caaggattgt gacataacgc caggcggagt cacgcacatc
     5401 ccattgcaca ccgggataat attggttgcc atgcccctgc cccggtatag tgcggctaat
     5461 gatgctgcca cagccactca gcaaggtcac catgatgctc accacaatta aacgcatttg
     5521 atacctcttc cgtaaaaaaa taccggcata acgccggtat ttttattggc tcgttgtttt
     5581 tgccagatgc gacgtgaacg ccttacccaa cccacaaaat cttgcaaaat caataaattg
     5641 caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt tattgcgaag
     5701 ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa taactcaccc
     5761 atgaagaata tctctcaggg gaattccaga cgcggaaatg cagacgggcc atcgtcaccg
     5821 gatcgcttag cagcacaagc cgacgatcgc gattcagctt ctctggcgtc tcgttcagcg
     5881 cctgttcaac gtggcggtca cgggcgattt ccagcacctt actggcgcgg tgacgcgcgg
     5941 tcgccattgc ggttgccaga gcgttaaatg acgggttaaa cactgcgtgc ataaaaccat
     6001 catcaaggga gcgttgacgg ttcatctcaa ggaaccgatc ggtatcaacc agtacctgcg
     6061 gcggtgaata ctcttccggg atcaggaaca gtttccagcg tttggtgcgc agaccaacgg
     6121 tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaaa acaatcggtg
     6181 ccagccagaa caggaaacgc agatccaacc acgccatacc aacagcccac actaacccta
     6241 acagcagctg tgagccgtgg cgtttgaacg cttcacccca ggaagtggag tcatcatcac
     6301 gctgcggtga attccacacc acttcccagc caaggaacgc gctgacgacg aagaccgtat
     6361 ggaacagcat gcgtaccgga gccagcagca cggaaaacag cacttccagc agcaacgata
     6421 atgtaacgcg ccagaagccg ccatattctt tcgttccttt gcaccaaata agcaaaatgc
     6481 tcaatagctt tggcaggaac aacagcacca tggtcgaagc aaaaagtgca atcgccagct
     6541 caggacgcca ctgcggccac accgggaaca actgccgtgg ttgcaggaag tattgcggtt
     6601 cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata aaccacagcg
     6661 gagcggagag ataagacatc acgcccgtca ggaacaccgc acggtgaacc gggtgcatac
     6721 ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg cggtcacgtt
     6781 ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga tcgtaagcaa
     6841 tccagacccc ccaacctgca cggcgcatca acgccgcttc cacgaagtca tgtgacagga
     6901 ttgaaccggc aaaggaacct tcgcccggca gcggagccag tgcgcagtgc tcgataaacg
     6961 gtttcacgcg gataatcgcg ttatgccccc agtagtgcga ctcgccaagt tgccagaagt
     7021 gcaaaccggc agtgaacagt ggcccataca cgcgggtcgc gaactgctga cagcgtgcat
     7081 acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg ttcgggttgg
     7141 cttccatcaa gcgcaccagg ccgcataaac aatcaccggt cattaccgag tcagcatcca
     7201 gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg atattaccgc
     7261 ttttacgctt cacgcggcga cggcggcggc gatagaaaat ctgaccttcg ccaccgactt
     7321 cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg ttataactgt
     7381 cactcagaat gtagacatca aagtgtttgg cattcccggt ggcttttact gattcccacg
     7441 ttgcacgcaa gccagcaaaa acacggttca cgtcttcgtt acagataggc atgatcaacg
     7501 ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat atactgtatt
     7561 tatcgcgacc aataagcagt tgcaggaagc ccattaacgc cgtccagaat ccggcggaca
     7621 cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata taaggcagaa
     7681 gctgcataaa ggaaacccac aaatcctgac caaccatatc cataggatta atcagcgccc
     7741 aaccctgata aggaagaatg gtcttcatat accaggtcgc gacgacagtt tgcgcgagcg
     7801 tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt tgctcgctct
     7861 cctgctcttc tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt ccacgcaggc
     7921 gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc gaggagcgtt
     7981 ttgcttctgg catcgccttc agctgatcgc gcccttcgtc gtctttaatt aactgtccat
     8041 cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct tgcggggagt
     8101 catcctcccg ctcccaggtg cggtgttcgg catccagcgc ctgatgaacg gcgcggatat
     8161 cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca atatactcag
     8221 ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt ctgatcggca
     8281 ttcaccagcg cagctcgcat ttcagtggtt ttcttggcat ctttcacttt cacacgcatc
     8341 accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttccac tatctcacca
     8401 ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt tttcatctct
     8461 gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcggat caggttcgac
     8521 tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc tggcgcatgc
     8581 agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc tttacccggc
     8641 tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttagtttc atcgttggtt
     8701 ggaatttcca ccagctcaac gctgcctttg ccccactccc ctttcggagt cacccatgcg
     8761 cttggacgga gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc acgaccgcgc
     8821 tgcaacagac caaagccttg cgggttttcc atggagaagc tgctgaccgc taaatgtttc
     8881 gggttattca acggacgcca gatccattcg ccattaccgg catggataga gagaccgtta
     8941 gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg cccaaacagg
     9001 aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag atagattttc
     9061 gactgcacat ccacaaccgt gtcacgccct ggcataacca cgaatttgta agcacctgtc
     9121 gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg ttttggacgc
     9181 tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc ggtatcaatt
     9241 gccaggccgc gggcagaaag gccataaacc tgacctgcac caatcacgcg gaaatagctg
     9301 gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg gtaaagcact
     9361 ttaaaaccag caaaaccaag gtcttttacc gtgtctttgt catgctgaac atcgccgaaa
     9421 gtgaaataat ccgggctgta tttgattcgt ttgacggcgg tggcagtcac ttcatttatt
     9481 ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa tggggtcttc
     9541 agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc cgcgtatttc
     9601 atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt cccggctaag
     9661 gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga tgtatacagg
     9721 gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt atgtttcata
     9781 attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt aatggataac
     9841 cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg gattaagcac
     9901 ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat cgacttcgtg
     9961 tagggtttgc cagcaaattt atttatcaac cgtcttacag acgcttccat tctggttagt
    10021 ggtagcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg tcaggtcggt
    10081 gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa aaatccgaga
    10141 ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc gactccatcc
    10201 gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat tcgagccata
    10261 catggcatgt gaatagcgcc gaaccgtcat tgtggctgac cctttttaat gacttcatcc
    10321 actcgttccg catgcaggta tttttcgtta tatccggcta cttttcctac atgctttttt
    10381 tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt atcccgatgt
    10441 taacagccat ccccctactg acattgccgc aatttattat gctgcaatac gtcaaaggga
    10501 aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgtta gcctgggaat
    10561 taatatcaca cctgtggttt ttactggtgt tagtggtcat gacgacgctg tgcgtatgga
    10621 tatttaaacg caccagaaat aatttagaaa attctgataa aacgaataaa aaattctcga
    10681 tggtaaaact atcggtgatt tttttgtgcc tcggcatcgg ttatgcggta ataagaagaa
    10741 cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt attgtcatgc
    10801 aaacgctatt ttatttaccg ttctttatcc tcggcgcact ggctttcatt ttccctcatc
    10861 ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct cgcagcagca ttggcatttg
    10921 tcgcttactt actcaaccag cgctatggca gtggcgatgc ctggatgtac gaaaccgagt
    10981 ctgtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc ttcggccacc
    11041 gtttgcttaa cttccagtca gcgcgggtga cttactttgt taacgcatcg ctatttatct
    11101 atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg cacatcacct
    11161 ccaactggct tggttttctc tgtggcctga tattcgtagt agggattgcg ataattctgt
    11221 atgaaattca tttacgcatc ccgttactga agtttttgtt ttctggtaaa ccggttgtta
    11281 agcgtgagaa cgataaagca ccagcccgtt aagccacatt tacaataacc attccacggg
    11341 caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg gttctttttt
    11401 gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc acctgtccag
    11461 acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa ttaactgtgc
    11521 cagcgtttcg ctctctatca caaagcccat ttcagtattg agcaatgttg aacgcggatc
    11581 gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaggttt tagcatgcag
    11641 gctggcaccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt gttcacgcgt
    11701 cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc agcgcgcgta
    11761 tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga tcgcaatctt
    11821 tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg gtacaaaata
    11881 ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca ggcgctgcgg
    11941 taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac ttaataaacg
    12001 tgttttcgcc cagataagcg gcaacgttcc atcaaccaga tgatttataa acggactgga
    12061 ttccattttg cgtaagtaac gatgcgccat ggcatcgtta tgccaggagg cgggtaactc
    12121 gatgcgatcc gccatttcac cttccgggac atccagcacc tgctgtaagg gtgaaaccga
    12181 tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctactacgg gtcctattgc
    12241 catgacatct aaatccgaaa aaagtggctc ttcccctgcc ccaaaatagg catcaccaat
    12301 atttcgcccc cctaccagcg tcaccacgcc atcgacagtg aaacttttat tgtgcatacg
    12361 gcgattgaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc gaaacgagaa
    12421 aggattaaaa agccggactt caatgcgggg atggctgtca agcaagcgta aaatgtcgtc
    12481 aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac gctttgctgc
    12541 ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga tgtaatactg
    12601 aacatccagc gtatgctctg ccatttcggc cagacgatac cgggcggcaa acgcatccag
    12661 acttttctcc agagggaaaa gaccacactg accgggatgt tgcgaacaca gtggcagcac
    12721 cgcgctcgcc agccggggca aatcattcat ctccttgttg ggtaaggagt ctttcgtaga
    12781 ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa gcgtgacggg
    12841 taataaattc tgaaacggtt ttaactgcga tttcagccgc tgccgcacga gggtaaccat
    12901 aaaccccagt actgattgca ggaaaagcca ctgacgtata gctgtttgcc gccaccagtc
    12961 gcaggctatt cagataggca tcctgcaaaa gctggtcttc gttttgttca ccaccacgcc
    13021 agactggccc gacggtgtgt actacggctt tagcgggaag atcgcctgca agcgtaataa
    13081 cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacag gcatccagca
    13141 gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccgcctccc attaatgacg
    13201 gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc tgcacaacat
    13261 gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt acagcaggct
    13321 ttttggttaa atcgcatttt tctgctcagc aacgtgcgtt gttcagaaac ttagttatat
    13381 tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt tactgaggct
    13441 atttgcgcgt ttttgacatt gccgctcatt aacgctttgc tggaataggt tttgcccgga
    13501 acagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct gacgtcacat
    13561 gccggttcca caatttggcc ataaaaatgg atcacacccc cttgcgcggt gcttcctgcg
    13621 atggcaataa aaggaaaaaa caaactgccg agcataagag agtttaaaaa aggtctgaac
    13681 atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc atactttcag
    13741 gttggctgat tcgcagcaga ccattctctc cagattcatc ttatgctcga tatttcaaca
    13801 aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtgc ctgtccatca
    13861 gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact caacttcgtc
    13921 aaagcaatag gttgattagc aggcaatgag agggtctttt cttgcttcgt ctgactttgc
    13981 cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga ttgagtaaga
    14041 gtgacttcag gaataatggt atacacatcc ccttgctggg tcgtattaaa ggttatctgg
    14101 ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa aaaaacaggg
    14161 cttgcgccct gtttctgtaa tacaaatgat gtattagtac tgatgagcgg tcgcgttgtt
    14221 accaaagcca atctgagtca cgttgacgga ggagttagat gcagtctggt caactgcagc
    14281 accgttgcca ccaccgaact gtttaaccgt catttcagaa tttttgccgt tccactgatc
    14341 aagagtggcg ctgttaccga agccacgttg ggtcagatcg attgagctgt catccgagcc
    14401 ctgaccaaca tctgcaccat taccgccgcc atgctgggta atagtcaagt cagaattacg
    14461 ggcatcagtt tgcagagcaa gtgcagagtt accgccaccg tactggtaaa tgttcagctc
    14521 agaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc cgtactgagg
    14581 aacaacacct gccagagcgc taccggagaa tacgattgct gcaattgctg ctacttttaa
    14641 aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat taacgttgtg
    14701 tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt tgagtaccat
    14761 actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca gtattaccat
    14821 aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata tatgcaaggt
    14881 tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc gcaacaaccg
    14941 ccaaaagttt tgagcctccc tgccgtaact gagcactatt attagtccca gcttgaccaa
    15001 ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag ttatattctg
    15061 aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt attgttaaca
    15121 tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac atttaagaaa
    15181 ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct gcgttacgat
    15241 ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttgtat ttttcttttt
    15301 ttgttcatac caaagtatta
//
LOCUS       DAPGRN010000113        14740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPGRN010000113 DAPGRN010000000
VERSION     DAPGRN010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.115"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1784)
                     /locus_tag="RHW33_003741"
     CDS             complement(<1..1784)
                     /locus_tag="RHW33_003741"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDV1647201.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIESFPSAVLAHR
                     WPHVANLGDMTKLAKKVLAGEIESPDVLVGGTPCQAFSIAGLRGGLDDERGALTLKYV
                     ELANAIDDKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPES
                     GKSNAFWRWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASA
                     RTDLDPATVLFEFEGVRRNIAPSRGEGKETTRYTSDIAIRSCDDTNIVAMAHGQGGAE
                     IKTDNSAPTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDGHTLIPTEKRKKVSSD
                     ELAYLRKNSPDLNEEEAAMLAADGPRYKAIGNSMAIPVMRWIGERITKAACRQNEGRE
                     TKERKVKPAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQE
                     NLLNDVNADLINFYKTLQREAHSLITLAHRFFQDYNTQEGFLAVRNAFNKQVYDDLHR
                     AAAFLFLNRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDF
                     AAVIEAAGEGDVIFCDPPYEPLPNTEGFT"
     gene            complement(1781..2686)
                     /gene="rdgC"
                     /locus_tag="RHW33_003742"
     CDS             complement(1781..2686)
                     /gene="rdgC"
                     /locus_tag="RHW33_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV1647202.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     VVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLDQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            complement(2679..2963)
                     /locus_tag="RHW33_003743"
     CDS             complement(2679..2963)
                     /locus_tag="RHW33_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647203.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGTNRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            3426..4214
                     /locus_tag="RHW33_003744"
     CDS             3426..4214
                     /locus_tag="RHW33_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647204.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSS
                     PGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANV
                     AITAYNHAIGTALAEASANKASTASAVAALVPDSVLDELNKAAQ"
     gene            4254..4676
                     /locus_tag="RHW33_003745"
     CDS             4254..4676
                     /locus_tag="RHW33_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HDV1647205.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     LDKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            4854..5246
                     /locus_tag="RHW33_003746"
     CDS             4854..5246
                     /locus_tag="RHW33_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647206.1"
                     /translation="MSDKVTVKQTINKATSIYKIEQITVGKPGSEQYRRAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDHCIGVICSSVSYELFPNVHE
                     DGGIGYQFLYEGDELKCYEHGLLIESIE"
     gene            5565..6425
                     /gene="repA"
                     /locus_tag="RHW33_003747"
     CDS             5565..6425
                     /gene="repA"
                     /locus_tag="RHW33_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HDV1647207.1"
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     gene            6825..8030
                     /locus_tag="RHW33_003748"
     CDS             6825..8030
                     /locus_tag="RHW33_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1647208.1"
                     /translation="MKRDYGGVGTIALRASALLKAMSQDIEDQRKEFNQTEYYQTFTR
                     NAVAKLPKLSRRIVEQAIKEMEDDGYQFNKKQVGNVEQYALTIQNVIDIYAHRKIPKY
                     RDIHKSPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGSILETAAQAMLNDLDAETLRKEVIRPTIVPGVDVIPASIDDGFVASQWK
                     ELVEEHLPGQNQYEILRRNIIDRVADDYDFIFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLEQLEEEGVEPRLSASIGFMSKMTGKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTVISANPQSYPGSAEALKKARTEAERFTKAVFDR
                     IEFVRGEAA"
     gene            8027..8992
                     /locus_tag="RHW33_003749"
     CDS             8027..8992
                     /locus_tag="RHW33_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV1647209.1"
                     /translation="MKKIVSRGRVLGKNSSEFARMLEGSEGTKTFTLKSGRQAKFLLT
                     VVLSGEIESRTFVDPAVNGRDQSLLTPESVSDISRTIKLQQFFPAIGRMVGERIEVLD
                     GSRRRAACIFNETKFEILVTKDEISLADARQLAIDIQTAREHTLRELGKRFEVMYGKS
                     MTKEEIARAENISKAKVTRAFQAAAVPDEMIAVFPVASDLALPDYQLLLQISEDANAK
                     SVPIEELVDTVRERIAETEGAKEDKAKILAIFKAESKSLKPAPVKSVVVEKLRDFSDR
                     RQYARKKSDPKKRVVAYEFSRLPSEVQTEIDEAIKKIIGKMSAGE"
     gene            9257..10963
                     /locus_tag="RHW33_003750"
     CDS             9257..10963
                     /locus_tag="RHW33_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647210.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPIFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            11024..12613
                     /locus_tag="RHW33_003751"
     CDS             11024..12613
                     /locus_tag="RHW33_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647211.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EDLISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGKICADRLIDGFFDVKPTLTYTEAISAVEDTGLLGTDYVSCAVYHFPFSCKD
                     KWTQSRVVFGLSGSAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEA
                     VWACCPTGVARRIQGVPLLIK"
     gene            12623..13438
                     /locus_tag="RHW33_003752"
     CDS             12623..13438
                     /locus_tag="RHW33_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647212.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSDPAPEQILEEKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDMIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            13474..14055
                     /locus_tag="RHW33_003753"
     CDS             13474..14055
                     /locus_tag="RHW33_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647213.1"
                     /translation="MAPIPYGVYSQADGVSPFLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALF
                     DPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRRINIIDVAIQDISFDLDA
                     PRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            14067..14576
                     /locus_tag="RHW33_003754"
     CDS             14067..14576
                     /locus_tag="RHW33_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDV1647214.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
BASE COUNT         3844 a         3604 c         3570 g         3722 t
ORIGIN      
        1 gtgaatccct ctgtatttgg aagcggttca tacggcggat cgcaaaagat gacatctcct
       61 tctccggcag cttcaatcac cgctgcaaaa tcaccgcata caaactcaga ccgcccttcc
      121 gcaccgagga aggcttccat ctcctgtaat gggaaatacg gagttttata cttcccataa
      181 ccgacattga actcaccggc ctggttgtaa cgcgtcaatc cgttaaaaca atgtcggttc
      241 aggaacaaaa acgccgctgc gcgatgtaaa tcatcataga cttgtttgtt aaacgcattc
      301 cgtactgcca ggaatccctc ctgggtgttg tagtcctgga agaaacgatg tgccagagtg
      361 ataagtgaat gcgcctcgcg ttgcagagtc ttgtaaaagt taatcaggtc agcattcaca
      421 tcatttagca gattttcctg gtatccggca ttcatgaaga cagctccacc accgacgaaa
      481 ggttcaatca ggcgcttccc ttctggcaaa tagcgaaaga tttgttccag aacaccaaat
      541 tttccaccag cccatttgaa tatggaccgt tcgaattctg ccgctggttt aacttttcgc
      601 tcttttgttt cacgcccttc attctgccga catgcagcct tggttatccg ctcgccaatc
      661 cagcgcatta ctggtatcgc catactattg ccgatcgctt tgtaacgcgg tccgtcagct
      721 gcaagcattg cggcctcttc ttcgttcaaa tcaggagagt ttttgcgaag gtatgccagt
      781 tcatctgaag aaactttttt acgcttttcc gtagggatca atgtatgtcc atcaggaaaa
      841 ccttgcagcc tttcacattc gacaggggta agacggcgca ttctaccgtc gccgagcaat
      901 acaattggtg cttcatggtt acatgtcaaa gttggtgccg aattatcggt ttttatctca
      961 gcccctcctt gcccatgtgc catggcaact atgtttgtat catcgcaaga tctgatagcg
     1021 atgtctgaag tatatctggt agtttccttc ccctcgcctc tgctcggcgc aatattccgg
     1081 cgcacgcctt cgaactcaaa aagtaccgtt gcgggatcga ggtctgttcg agcacttgcg
     1141 acaacaaaca cgcgtcggcg tcgttgtgcc actccgaagt attgggcatc aaggattctc
     1201 caggccacct ttcgctgcgg tccataaata caaccacact gcggccactt tggaacatgg
     1261 caaccggttt tgccatccca ccgccagaac gcgttgcttt ttcctgattc aggtcgatca
     1321 cctggttcaa atggcacatc ttctccagcc aatccagcaa ggaaacatcc gaaggcgtta
     1381 tctgccgatg acaagactcc tgggacattt tcccagacga taacggctgg tttgagaaat
     1441 gactcagccc gtttgtcgtc aattgcattt gcaagctcca catactttaa agttagcgcg
     1501 ccacgctcat catcaagccc accacgtaat cccgcgatac tgaatgcctg acaaggtgtt
     1561 cccccgacga gcacatcagg ggattcgatt tccccagcca ggactttttt ggcaagtttt
     1621 gtcatgtcgc caaggttggc gacatggggc cagcggtgcg caagaacggc agatggaaaa
     1681 gactcgattt cagcaaacca cgcaggacgc atacccaacg gttcccaggc aatactcgcg
     1741 gcttcaattc cactgcaaac agatccatag cacagctctt tcactgctta gcctctccac
     1801 caagggcatt taccagagca tcaaccaggc acgaaatttc actggtcaac aggaagaaat
     1861 ctgcgtccag tcgctgcgct acatcttcac tatcaatatc agagttctga tcaagcaatt
     1921 catccgcaaa tttgacgctg gtaaggctga agttatggtc cagtgtaaat ttaatgcggt
     1981 tctgccagtc gagtgccaac ttagtgacga gcttgccagc ttccaggtgt gtggaaattt
     2041 catcgcttcc caaatcctgc tttttcactc gggcaatacc gccatcctca agcactgcct
     2101 taagttctgc cgcatccccc atttgaaatc cctgtggagc actaccatca cgtacccagt
     2161 cggtcagcgt taattcaatg ggattttcaa cactcagggg aacaacagga agagaaccca
     2221 gagacttacg cataagcgcg agcatatcct ctgcctgccg cgcgctggca ttgatataga
     2281 tacgtttagt tgaaccgtcg tagatcgcct ggataacaga aaactttgaa aaagcccgtg
     2341 gcagaagaga atgcagaact tcgtctttca gggagtcctt ctctgttttc ttcagtttac
     2401 gcgcttgttc ttgctcaagt ttttcaattt tttcttgaat agctcgctgg ataaccggcg
     2461 ggggaagaat ttttgtttcg cgctttgctt caacaaggat aaaaccattt ccatgcatag
     2521 cgataacttc ggaattatca ccaaatggag atacaaaacc gaacttggcc atatcctgac
     2581 taccgcatgg cgtgaaaagg atcattttct ttttatcttc taagtcggtc agatccgcct
     2641 cacgagaaag tttataaata gtaatgtttt tccagtgctt aaacatgttg taacccttga
     2701 atatcaacca cagaaagctc gtctttgtag aaaaaggcca ggttgtggca ccccctcgtt
     2761 tgagcgtatg agctgggacc aatttcgttc ttccagacaa atggcttcaa atccgtacgg
     2821 cgaagcataa aaacgcgatt tgttccgctc tgattcccaa tgaggcaaaa gccttctttc
     2881 accttgatag cctgcaagtt gtcgagttca ccgctggtta cacggctatc gaactctttg
     2941 cggcttatta gctccatctg catctgacga ctccaaacaa atgcccattg aagggcgatg
     3001 gctgaatggt atcgaaaata cgacataaaa aacaatattt attagagcaa ttttgcaata
     3061 gttgaactcc atgtagacca caaacaacct gagttaaaat aacgaaaatc agagcaaata
     3121 attggtgatg acgtggcaag tattgcaaca aaagacagca tttgttcggg gcacggagga
     3181 ttcccatcca ggcctcctgt agagagtgaa ccactactta aagtcaacgg agtcgaagtg
     3241 ttagttgatg gtaagcaata tgcacagcat accgatggaa acagtacgca cggtgggcaa
     3301 gctatatcaa ccagggcatg gtttaccgtc aatggtaaag ggatcgtatg cgttggtgac
     3361 cctgtttcat gcggatctac cgtagcgtcc ggagacggcc tggttcaggt aagttaggag
     3421 atatcatgct ggaaaaagac taccagttat ccgcatataa aaaattggcc gccgccggtg
     3481 ggatgaaaac acctggtgcc ataacatcgg cacgaaacag tgctaacaca gcaaaactgc
     3541 ttgcagaaga attgaccgga ttaattctgg atacaattgt ctatcccgac actattacca
     3601 gctatgtttc aacgatcaga acaaccacaa ccggcttaac gaacattgga gaactggcaa
     3661 ctaagcacgc ggacctgttg gctggttatg cagatctgtc aatgctgctt caactcgata
     3721 ttggttggga tgtttactgc cgtgctaatg agcgagaagt ttcagaactg ccgatctcta
     3781 ttgccattgg tgatgtgaat attactaaat cgcttgagga cgctgttaac gcgcttaata
     3841 catcaagttt agtcgctgca atgggggaga ttaaccagac ccttaacact ggctcaggaa
     3901 gctcgccagg ctctggttca ggcggcggca ctgccactcc cccaccagca ctaacagaag
     3961 agcaaattga atctctgaaa gtagcaactg aacagtttgg ggttgttttc aaccagacaa
     4021 cagcgcccac aactgcgtta caacagcagt atgaacgagc gaatgaaagc gccaatgtag
     4081 ccataactgc ttataaccat gctatcggta ctgcgcttgc ggaagcatca gcaaataagg
     4141 ccagcacagc cagcgcagtt gctgctttgg ttcctgattc tgttcttgat gaattaaaca
     4201 aagcggcaca gtaacaaagg acttcattga taatttttct tcaggaggaa gacatgtcat
     4261 tcttttctac gttaaaaaca gctttgtctt tgaaggagaa acttgctgct actggtgttc
     4321 ttgttctgat ttgcgcactt gttggtgccg ggtttgcatg ggaacgtcat cagctaaagc
     4381 aagccttgga taaaattggc agtcttgatc aggctgttaa ggaacgtgat aagtcaataa
     4441 tggatcttaa ccagaccatt gagacgatga acaaagcaga gcaacatttt cacagccagg
     4501 aagtgaaaaa tgaatcagaa caagccaaat atgctgacag gcaaatggaa cgaaaagctg
     4561 aagttcagaa acaactggtt gcggcgggta atgttcgcca gcgtattcct gctgacactc
     4621 agcggttgct ccgggagtcg atcagcgaat ttaacgccga cgccgacaaa ggttaaccac
     4681 cctgacccaa aaagtgcatt catgtgcagg atgccagagt ttagcagtga atattttgat
     4741 gatctgccag cgtatatcct cgatacagaa acgatgctga tggggattaa caggaagaat
     4801 cgcaacgtta atgattacaa ccgagctatt agcggtaact aaaagggatt tttatgtctg
     4861 ataaagtaac agtaaagcaa actatcaaca aagcgacttc aatctacaaa attgagcaaa
     4921 tcactgttgg caagccagga tctgaacaat accgtcgtgc tttcgagctt gccgatcagc
     4981 ttggtttaaa acacccggat tgcattgagc atgtatttcc gacctatgct gatgagcaat
     5041 gtactcatgt tcttaccgaa gaggattttt tcagcactga agaacgagaa ggcgttgatc
     5101 actgtattgg tgtgatttgc tcttcagtga gttatgagtt attccctaat gtccatgaag
     5161 atggtggtat tggataccaa ttcctgtacg aaggcgatga gcttaaatgt tatgaacatg
     5221 gtcttctcat cgaaagcata gaataataca gcttccttcc aaccggcttt gttggccggt
     5281 ttttcactta tccacattat ccactggata gatccaataa ttaggtccat acagatccca
     5341 attagatcca tatagatccc tgatcgttgc aggccgcgcc acgtctggcc tagaagtgta
     5401 tcgcgatgtg tgctggaggg aaaacgatgt gtgctggcgg gataaaaatg tgtgctgacg
     5461 ggttgctaat gtgtgctggc gggatatagg atgtgtgctg acgggaaagc ctgggtagtt
     5521 atcatcactt ataaaaacta tccacataat tcggaaaaag taatatgaat caatcattta
     5581 tctccgatat tctttacgca gacattgaaa gtaaggcaaa agaactaaca gttaattcaa
     5641 acaacactgt gcagcctgta gcgttgatgc gcttgggggt attcgttccg aagccatcaa
     5701 agagcaaagg agaaagtaaa gagattgatg ccaccaaagc gttttcccag ctggagatag
     5761 ctaaagccga gggttacgat gatattaaaa tcaccggtcc tcgactcgat atggatactg
     5821 atttcaaaac gtggatcggt gtcatctacg cgttcagcaa atacggcttg tcttcaaaca
     5881 ccatccagtt atcgtttcag gagttcgcta aagcctgtgg tttcccctca aaacgtctgg
     5941 atgcgaaact gcgtttaacc attcatgaat cacttggacg cttgcgtaac aagggtatcg
     6001 cttttaagcg cggaaaagat gctaaaggcg gctatcagac tggtctgctg aaggtcgggc
     6061 gttttgatgc tgaccttgat ctgatagagc tggaggccga ttcgaagctc tgggagctgt
     6121 tccagcttga ttatcgcgtt ctgttgcaac accacgcctt gcgtgccctt ccgaagaaag
     6181 aagctgcaca agccatttac actttcatcg aaagccttcc gcagaacccg ttgccgctat
     6241 cgttcgcgcg aatccgtgag cgcctggctt tgcagtcagc tgttggcgag caaaaccgta
     6301 tcattaagaa agcgatagaa cagcttaaaa caatcggcta tctcgactgt tcaattgaga
     6361 agaaaggccg ggaaagtttt gtaatcgtcc attctcgcaa tccaaagctg aaacttcccg
     6421 aataagtgtg tgctggaggg cagctgcatt tgaaaaatgt gcgctgccgg gaatgcctgc
     6481 ccattttcct gtttttggtg tgcgctggag ggttgcgcca cgcagtttgc ccagacattc
     6541 cctccagcac acatctaacc atccaagttt ccctccagcg cacatctaat cttctatctt
     6601 tcccttcagc gcacatattt gataccagcg atccctccac aacacataat tcaatgcgac
     6661 ttccctctat cgcacattct ggtcctgcat catccctcca gcacacatct aatagcctca
     6721 tcgccatttc tttacgtgca ataatttacg catgaatcaa aataagttgc acgtagcaga
     6781 atcaaacgta caattcactc atgcgaaatg ataaggagat gatgatgaaa cgcgattacg
     6841 gcggtgtcgg caccatagct cttcgtgcaa gcgcattact taaggccatg agtcaggata
     6901 ttgaagatca gcgcaaagag ttcaatcaga ccgagtatta tcagacgttc actcgtaacg
     6961 ctgtggcaaa gttgccgaag ctgagccgcc gcattgtgga gcaggccatc aaagagatgg
     7021 aagatgatgg gtaccagttc aacaagaaac aggtcggtaa cgttgaacag tacgcgctga
     7081 ccatccagaa cgtcattgat atctatgccc accgtaagat cccaaaatat cgcgacattc
     7141 acaaatcgcc ttacgttatt tttgtcgtaa acctgaaggg tggcgtatcc aaaacggttt
     7201 ccacagtcac gttggcgcac gctctgcgtg tgcatcagga tttactgcgt cacgatctgc
     7261 gcattctggt aattgacctt gaccctcagg catccagcac aatgttcctc gaccatactc
     7321 acagtattgg ttccatcctg gaaaccgccg cgcaggcgat gctgaacgac ctggacgcgg
     7381 agacgctacg caaagaggtg attcgtccga ccatcgttcc tggcgtagac gtgattccag
     7441 cctctatcga cgatggcttt gttgccagcc aatggaaaga gctggttgaa gagcatcttc
     7501 ccggacaaaa tcagtacgaa atccttcgac gcaatatcat tgatcgtgtt gcggatgatt
     7561 atgactttat ctttattgat accggtccac acctggatcc gttcctgctc aacggtctgg
     7621 cggccagcga tttgctgctt acccctaccc caccagccca ggttgacttc cactcaacac
     7681 tgaaatatct cacccgtctg ccagaaatgc tggagcaact ggaggaggaa ggcgtagaac
     7741 cgcgtttaag cgccagcatt ggttttatgt cgaagatgac cggcaagcgc gatcacgaga
     7801 catcacacag ccttgcgcgt gaggtttacg ccagcaacat tctggactct tctctgcctc
     7861 gtctggatgg atttgagcga tgcggcgagt ctttcgacac cgtaatcagt gccaacccgc
     7921 aatcgtatcc aggcagtgca gaggcgctga agaaggcacg aaccgaggcc gagcgtttca
     7981 ctaaggctgt gtttgatcga attgagtttg ttaggggtga ggcggcatga aaaaaatagt
     8041 ttcccgtgga cgagtgctgg gcaagaatag ctccgagttt gctcgcatgc ttgaaggcag
     8101 tgaaggcacc aaaaccttta ccctaaaatc tggccgccag gctaaatttt tgcttaccgt
     8161 tgtgctgagt ggtgagattg agtcgcgcac gttcgttgac ccggcagtta acggccgcga
     8221 tcagtctctg ctcacccctg agtcggtaag cgatatttcc cgcaccatta aattgcaaca
     8281 gttcttcccg gctatcggtc gtatggttgg ggagcgcatt gaggtattgg acggatcacg
     8341 tcgccgcgct gcgtgtatct tcaatgaaac gaaatttgag attctggtga cgaaagatga
     8401 gatcagcctg gcggatgcac gccagctggc cattgatatc cagacagccc gcgaacacac
     8461 cctgcgcgaa ctgggtaaac gcttcgaggt tatgtatggt aagagcatga ccaaagaaga
     8521 gatcgcccgc gctgagaaca tttcaaaggc taaagttacg cgcgctttcc aggcggcagc
     8581 ggtgccggat gagatgattg ctgtcttccc tgttgccagc gatctcgccc tgccagatta
     8641 ccagttactg ctccagatct ccgaggatgc caacgctaaa agcgtgccta ttgaagagct
     8701 ggttgatacg gtgcgcgaac gaattgcaga gactgagggc gcgaaagagg ataaagcgaa
     8761 gatactggct atcttcaaag cggaaagcaa aagcctgaag cccgcgccgg ttaaatctgt
     8821 ggtggttgag aagctgcgag acttctctga ccgtcgccaa tatgctcgaa agaagtccga
     8881 tccgaaaaaa cgggttgtcg cctacgagtt ctccagactc ccgtctgaag tgcaaactga
     8941 aattgacgaa gcaataaaaa aaatcattgg gaaaatgtct gctggggaat aatcccgctg
     9001 gtgggaggtg gtgttagccc ctccccttcc taaaatgtcc cgcaccaatt tcattgctaa
     9061 cattatgatt tgcatgtgtt ttttaggaga atttcagact gaaattccca cgatttaacg
     9121 cctgcattca ccgtccactt ccccgcacaa aaaatttaaa aaattacttt tcgcgagaaa
     9181 gtcaacaagt gactttcaat aaaatctctt ccgaaaaggg attcacacaa gtgccttgtg
     9241 tttaaggaag agtaacttga gtaacttacg cgaataccag aatcgtattg cagatatcgc
     9301 aaaacgctct aaagccgttc ttggctgggc aagtacagca cagttcggta ctgataacca
     9361 attcatcaaa gatgatgccg cgcgtgccgc atctatcctt gaagctgcac gtaaagaccc
     9421 aatttttgcg ggtatctctg ataatgccac cgctcaaatt gctaccgcgt gggcaagtgc
     9481 actggctgac tacgccgcag cacataaatc tatgccgcgt ccggaaattc tggcctcctg
     9541 ccaccagacg ctggaaaact gcctgattga gtccacccgc aatagcatgg atgccactaa
     9601 taaagcgatg ctggaatccg tcgcagcaga gatgatgagc gtttctgacg gtgttatgcg
     9661 tctgccttta ttcctcgcga tgatcctgcc tgttcagttg ggggcagcta ccgctgatgc
     9721 gtgtaccttc attccggtta cgcgtgacca gtccgacatc tatgaagtct ttaacgtggc
     9781 aggttcctct tttggttctt atgctgctgg tgatgttctg gacatgcaat ccgtcggtgt
     9841 gtacagccag ttacgccgcc gctatgtgct ggtggcaagc tccgatggca ccagcaaaac
     9901 cgcaaccttc aagatggaag acttcgaagg ccagaatgta ccaatccgaa aaggtcgcac
     9961 taacatctac gttaaccgta ttaagtctgt tgttgataac ggttccggca gcctacttca
    10021 ctcgtttact aatgctgctg gtgagcaaat cactgttacc tgctctctga actacaacat
    10081 tggtcagatt gccctgtcgt tctccaaagc gccggataaa ggcactgaga tcgcaattga
    10141 gacggaaatc aatattgaag ccgctcctga gctgatcccg ctgatcaacc acgaaatgaa
    10201 gaaatacacc ctgttcccaa gccagttcgt tatcgcggct gagcacacgg tacaggcggc
    10261 gtatgaagca cagcgtgaat ttggtctgga cctgggttcc ctacagttcc gcaccctgaa
    10321 ggaatacctg tctcatgaac aggatatgct gcgtcttcgc atcatgatct ggcgtactct
    10381 tgcgaccgac acctttgaca tcgctctgcc ggttaaccag tcctttgatg tatgggcaac
    10441 catcattcgt ggcaaattcc agactgtata tcgcgacatt attgagcgcg ttaaatcttc
    10501 tggtgcgatg gggatgtttg ctggtgctga tgcagcatct ttcttcaaac agttgccgaa
    10561 ggatttcttc cagccagccg aagactatat ccagactccg tatgttcact acatcggtac
    10621 cctgttcggt aacgtgaaag tgtacgaagt acctgctggt atttgtaaga acttaacgac
    10681 agagaacatt cagttcagct cgatggatgt gctgtgctac gtccgtgatg aaaatccggg
    10741 taaagcaggc ttcgtgactg gtgatgctgt cccggctatc ccgttccagc atccgaccac
    10801 tccggcgctg gtcaaccgta ccacactgtg gggttcggct atcaacgata tgcacccacg
    10861 caacggcgct gattacttca ctcgtgtaac gctgacaatg gccaaaaaag gcgggcttaa
    10921 cttcataagc ggcgacacga ttgatgccgg tgactctgag taatcagggg aagttctccg
    10981 tttaacatag cgcccccgtg cggggcgcat aacagggaaa gttatgtctc aatattcaat
    11041 tcaacagtca ttaggtaatg catccggcgt cgcggttagc ccgatcaatg ccgatgctac
    11101 gttatctacc ggtgttgcat taaatagcag cttgtgggct ggtattggcg tatttgcgcg
    11161 tggcaagccg tttactgttc ttgcggttac tgagtccaat tacgaagatg ttctcggcga
    11221 accgctgaag ccgtcttccg gctcacagtt cgaaccaatt cgccatgtgt acgaagctat
    11281 tcagcaaacg tctggttatg ttgtccgtgc tgttccggat gatgcgaagt tcccgattat
    11341 tatgttcgat gaatcaggcg aaccggctta cagtgcgttg ccatatggtt ctgaaattga
    11401 acttgatagc ggcgaagcct ttgctatcta cgttgatgat ggtgatccgt gtatttcacc
    11461 tacccgtgag ttaaccatcg aaacggcaac agcggacagc gcgggtaatg aacgcttcct
    11521 cttaaaactg acccagacga cttcgctcgg tgtggtaacg accctggaga cacacactgt
    11581 gtctttggcg gaagaagcga aagatgacat gggccgcttg tgttatctgc ctacggctct
    11641 ggaagcccgt tctaaatatc tgcgcgcggt tgttaatgaa gatctgattt ctacagcgaa
    11701 agtaacaaac aaaaaatcgt tggcgttcac tggtggtacc aacggtgatc agtcgaaaat
    11761 ctcaaccgct gcgtacctgc gtgcggtgaa agtgctgaac aatgcgccgt acatgtacac
    11821 cgctgttctt ggcttgggct gctatgacaa tgcggctatc accgcattag gtaaaatctg
    11881 tgcagatcgc ctgattgatg gcttctttga tgtcaaaccg acattgacgt acacggaagc
    11941 gatctctgct gttgaagata ccggtttact tggtaccgat tatgtaagct gtgctgtcta
    12001 tcacttcccg ttctcctgca aagacaaatg gacccaatcc cgtgtggtct tcggtctgtc
    12061 tggctcggcg tatgcggcga aagctcgtgg cgtcaagaaa aactctgatg tcggcggttg
    12121 gcattactca ccggctggtg aagaacgtgc cgtcattgct cgtgcgtcaa ttcaaccgct
    12181 gtatccggaa gataccccgg acgaagaagc aatggtcaag ggccgtctca ataaagtatc
    12241 tgttggcacc tctggccaga tgatcatcga cgatgcttta acttgctgca cgcaggataa
    12301 ctatctgcac ttccagcacg tcccatccct gatgaatgca atcagccgtt tctttgtcca
    12361 gttagcccga cagatgaagc atagcccgga cggtattact gcggctggtc tgactaaagg
    12421 gatgaccaaa cttttagatc gctttgtcgc ctccggcgct ctggtggctc ctcgtgatcc
    12481 tgatgctgac ggtacagaac cgtatgtgct gaaagttacg caggcggaat tcgataaatg
    12541 ggaagcagtc tgggcctgct gcccgactgg cgtagcccgt cgtatccagg gcgtaccgct
    12601 gcttattaag taagggaata caatgagcaa aaactttttt caatccgggg catttttggg
    12661 gaatggactg tcccgtttcg ctttgaactc tgatcctgtg cagctgatgg agtctgcccg
    12721 agcaagcgcc gaaccgccaa cagatccggt tattaataat aatccggaac cggcggcaca
    12781 gactaacgat aacgttccat ctgatccggc tcctgagcaa atcctggaag agaaagacgg
    12841 taaagaatgg accgttgaac aggcgcacca gatgattctg gaagctgcaa atcgaagtgc
    12901 gatgcagaat gcgttgagtg atgcggccga cgccgttttc gcctgggctg atagcggtga
    12961 tctgactttc gactcccttg atggtttcgt tcaggctatc gctggtatct ctgatgacga
    13021 cgactccgaa gttacagaag aacaggacga tgcctataac gaagcatggg caaatgttgc
    13081 tgacttcctc gcagcatgcg gtgtagatga tgacatgatc gaagcactgg ctgacgatga
    13141 agacgacgac gctgctgctg atgttggtgc ctctatcgct ggtttagata gcgacgaccg
    13201 cgacgaactg gaagcggcgt ttgttgttgc tggcacttct gatgaaatgc tgactgaagc
    13261 atttaagaag gttgttcgta acggtgagat caaactcatc cgtaaacgcc tgcggaaaaa
    13321 acgtctgact gcggctcaaa aatcggcgct gaaaaaagcg cgtcgaaaag cccagaccgg
    13381 cgcggcaaaa cttgcccgca aaaagtcaat gaaactgcgc cgtaagcgcc ttggctaaag
    13441 gaggaggccg gagaactccg gcctttaact tgaatggcac ctatacctta tggggtttac
    13501 agccaggctg atggtgtatc gccatttctg aaagttactt taacgaactc tcagtaccag
    13561 gttaccggat atatcagcca gggggcagca atgaacatgg cccagaattg ggaagcgccg
    13621 tttaccggta tgtccatggg gtctgttgct ggtgctttca gtggttttgc gcaggttggt
    13681 actgaaacaa cgtcggtggc ccgttggaac agcttaatgg tttgggaggg gggaacaccg
    13741 ccgactttca cgctgccagt aactttcatc gctttgtttg acccattcac ggaggtttca
    13801 ggagctatcg ccgcattgtc agcgatgatt agcccggaac ttaaagatgc cagtattggt
    13861 ggtcgaatcc cggagcgtgt gacgctaaac attggtcgcc ggatcaacat cattgatgtc
    13921 gctatccaag acataagttt cgatctcgat gcacccaggg acagcaatgg gcatttcctg
    13981 aaaaacaccg tcaacctcca gttgaccggt tcttcgatat ataacagctc cgatattgtt
    14041 cgggcgttcc agtaaaagga ttttatatgg ggcacaataa cactaaggga aaccgtaaat
    14101 ttattaaggg ccgctatact gccaacgcgg ccaaaggcga acgactggta tcttctgaat
    14161 tccagctcac ttttgcaggc catgaagata tcagcgtact ggttcgcacg tcgcaaattc
    14221 ctgaaatgac ccgcgaggat gtggaggact atggtccgaa tggtgtgaag ttcaaccagc
    14281 acggtccaat tcgaaactct ggggaaatcc aggtccagtg cgtggagact atcgaaggcg
    14341 atattcttca gttcattaaa gatcgcattg cggcgaagga ctatgttgat atcacgatgg
    14401 ctgctacccc tgaatccaaa tcttccgggg ttaacgctgt gacaaaagct gctacaacaa
    14461 ttgaaatgtt ggactgcaaa atctacagtg atgcaatcga ctttagtacc gaagatgtga
    14521 ctgccgctgt gcgcccgtca cttcgtatcg tctacaactg gattgagtgg gattaagtgt
    14581 tatcccttgt acttttaatc ttcttcgggg gaggtttacc tccccttgat acaaccgcat
    14641 tccaccgcct agcgaataga gtaaaataac atcttgtaaa tactctggta tgaagtgagt
    14701 gtaaactaac agttacctat taataaatac tcctatactt
//
LOCUS       DAPGRN010000114        14611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPGRN010000114 DAPGRN010000000
VERSION     DAPGRN010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.116"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            363..572
                     /locus_tag="RHW33_003755"
     CDS             363..572
                     /locus_tag="RHW33_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1647215.1"
                     /translation="MLRTSSAGNALLVCKLNRLGRSMWHLVVLLEELCERGINFRALA
                     QSIFAQQWGDECCKSKTICDLKVIV"
     gene            727..846
                     /locus_tag="RHW33_003756"
     CDS             727..846
                     /locus_tag="RHW33_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV1647216.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            911..5479
                     /gene="ypjA"
                     /locus_tag="RHW33_003757"
     CDS             911..5479
                     /gene="ypjA"
                     /locus_tag="RHW33_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin-like autotransporter YpjA/EhaD"
                     /protein_id="HDV1647217.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMAAFTTNVIGVVNDETV
                     DGVQKVDERGTTNNTHIINHGRQEVYGGISNSSIIETGGEQLVSIHADINGPANNTTI
                     NGGRQSIEYGGISTGTIIESGNQYVHKGGTSNDTTIKGGTSRIEGGTANGTIIDGGSQ
                     QVTTQGHVDGTTINKSGSQDVVQGSLATNTTINGGRQYIEQSTVETTTIKNGGEQRVY
                     ESRALDTTIEGGTQSLNSKSTAKNTQIYSGGTQIVDNTSSSDVIEVYSGGVLDVRGGT
                     ATNITQHDGAALKVTTYDLTVSGTNSEGAFSIHNKVADNVLLENGGHLEVSHSANKTI
                     IKDKGTMSVLTNAKADATRIDNGGVMDVAGNATNTIINGGTQNINNYGIATGTNINSG
                     TQNIKSGGKADTTIISSGSRQVVEKDGTAIGSNISAGGSLIVYTGGIAHGVNQETGSA
                     LVANTGAGTDIEGYNKLSHFTITGGEANYVVLENTGELTVVAKTSAKNTTIDAGGKLI
                     VQQEAKTDSTRLNNGGVLEVQDGGEAKHVEQQSGGALIASTTSGTLIEGTNSYGDAFY
                     IRNSEAKNVVLENAGSLTVVTGSRAVDTIINANGKMDVYGKDVGTVLNSAGTQTIYAS
                     ATSDKANIKGGKQTVYGLATEANIESGEQIVDGGSTEKTHINGGTQTVQNYGKAINTD
                     IVSGLQQIMANGTAEGSIINGGSQVVNEGGLAENSVLNDGGTLDVREKGSATGIQQSS
                     QGALVATTRATRVTGTRADGVAFSIEQGAANNILLANGGVLTVESDTSSDKTQVNTGG
                     REIVKTKATATGTTLTGGEQIVEGVANETTINDGGIQTVSANGEAIKTKINEGGTLTV
                     NDNGKATDIVQNSGAALQTSTANGIEISGTHQYGTFSIAGNLATNMLLENGGNLLVLA
                     GTEAHDSTVGKGGAMQNLGQDSATKVNSGGQYTLGRSKDEFQPLARAEDLQVAGGTAI
                     VYAGTLADASVSGATGSLSLMTPRDNVTPVKLEGAVRITDSATLTLGNGVDTTLADLT
                     AASRGSVWLNSNNSCAGPSNCEYRVNSLLLNDGDVYLSAPATTNGIYNTLTTNELSGS
                     GNFYLHTNVAGSRGDQLVVNNNATGNFKIFVQDTGVSPQSDDAMTLVKTGGGDASFTL
                     GNTGGFVDLGTYEYVLKSDGNSNWNLTNDVKPNPDPNPNPNPNPKPDPKPDPKPDPKP
                     DPTPDPTPTPVPEKRITPSTAAVLNMAATLPLVFDAELNSIRERLNIMKASPHNNNVW
                     GATYNTRNNVTTDAGAGFEQTLTGMTVGIDSRNDIPEGIATLGAFMGYSHSHIGFDRG
                     GHGSVGSYSLGGYASWEHESGFYLDGVVKLNRFESNVAGKMSSGGAANGSYHSNGLGG
                     HIETGMRFTDGNWNLTPYASLTGFTADNPEYHLSNGMKSKSVDTRSIYRELGATLSYN
                     MRLGNGMEIEPWLKAAVRKEFVDDNRVKVNNDGNFVNDLSGRRGIYQAGIKASFSSTL
                     SGHLGVGYSNGADVESPWNAVAGVNWSF"
     gene            complement(5774..5902)
                     /locus_tag="RHW33_003758"
     CDS             complement(5774..5902)
                     /locus_tag="RHW33_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV1647218.1"
                     /translation="MRYFQKKIAHLTFKRPASIEKMNDIIAKHYPDQLPPSTNQRF"
     gene            6275..7606
                     /locus_tag="RHW33_003759"
     CDS             6275..7606
                     /locus_tag="RHW33_003759"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024700.1,HMM:NF024867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV1647219.1"
                     /translation="MKINHIEVSNFRKYKAARFSFHPEFTVLIGNNASGKTTILDAIA
                     LLLNTYFQGSGLTTGGGTLKKDDARYIHTRKEEQDFLEPQSPVWLKGVAALAGETCEW
                     VREMGDRGGKAKAFTAYGALEREGVAKGQSPSLPMLLYYGAGRLWDLHRDNKMDTTGS
                     QLGAYRYCLDPKSDQYSFIKWFIKLTMTELQRKTPVPALRAVERAVLQCAPGAKSFYY
                     DVGSETILIELKKEGLVRFSDLSDGYRNMVAMIADIAHRCARLNPQMGENVTFETKGI
                     ILIDELDLHLHPKWQRRVVPDLRKTFPNIQFITTSHSPFIIQSMNPGEIIDLDNILAD
                     EQPQSVPEGIASPAPSMSFSAKSIEDIVENVMDVPLPQRSQRYQEMHDVAVKYYQLLE
                     QGQNAEGAEVLRLKDELDALSAPFSDNVAYHAFLEMERAAAGLGRSRGEDE"
     gene            7606..8307
                     /locus_tag="RHW33_003760"
     CDS             7606..8307
                     /locus_tag="RHW33_003760"
                     /inference="COORDINATES: protein motif:HMM:NF013964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1647220.1"
                     /translation="MRPIERGGCPVDNNQQKIVFSHYSDARGELIKRMGQYCSYCEMK
                     LDASLAVEHVQPKKARGSNVVNQTLLLDWENFLLACTNCNSTKGNKPVVLTDYIWPDT
                     DNTFRAFVYSTGGIISAAPGPVHQEAKNMIALVGLDKKPDTADASDRRWSNRLETWGM
                     AERARTRLINRDSIELRETIAEHAYSQGYWSIWMTVFSHDSDMLRRIIAQYMGTSALC
                     FNNTGMALPRPQGKC"
     gene            complement(8498..8775)
                     /locus_tag="RHW33_003761"
                     /pseudo
     CDS             complement(8498..8775)
                     /locus_tag="RHW33_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243790.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
     gene            complement(8794..8880)
                     /locus_tag="RHW33_003762"
     CDS             complement(8794..8880)
                     /locus_tag="RHW33_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647221.1"
                     /translation="MPGVLAVSTERRQDHYGPYACLMSVPLY"
     gene            complement(8917..9399)
                     /gene="radC"
                     /locus_tag="RHW33_003763"
     CDS             complement(8917..9399)
                     /gene="radC"
                     /locus_tag="RHW33_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV1647222.1"
                     /translation="MMEQSLIPLTPVLPLTAQRTVKRALTLLDRHLRETGVAFTSTQA
                     ARDWLKLKMAGLEREEFMVLYLNQQNRLIAHETLFAGSISSTEVHPREVVKSALYFNA
                     AAVILAHNHPSGDTTPSQADKTITQRLVQALQLVDIRVPDHLIVGGTQILSFAEHGLL
                     "
     gene            complement(9408..9866)
                     /locus_tag="RHW33_003764"
     CDS             complement(9408..9866)
                     /locus_tag="RHW33_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1647223.1"
                     /translation="MTTQTQCELMSANEPQFDLTATPVPDEQRLDFWPLYFGDIPQWV
                     SLEPRIFAWMDRFCDEYNGGIWSFYTLSNGGAFMAPDADSDDKWHLHNGMNGNGAEMS
                     AEAAGIAVCLIEYSHHACRTECDAMTAHYYRLRDYALQHPESSAILRIID"
     gene            complement(9952..10188)
                     /locus_tag="RHW33_003765"
     CDS             complement(9952..10188)
                     /locus_tag="RHW33_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDV1647224.1"
                     /translation="MSESHMLPPGSFTRQQAEAVTHRYQNIAIEDDQGSHFRLVVRDS
                     EGRMVWRAWCFEPDAGEGLNRYIRKSGMLRASSC"
     gene            complement(10266..10676)
                     /locus_tag="RHW33_003766"
     CDS             complement(10266..10676)
                     /locus_tag="RHW33_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647225.1"
                     /translation="MLKMMTSAVPALILSLAAFSAGAAQLPASLMDKDNPGQEYWCQG
                     NDINTSMCSLTGVSDDHKFALIQDLPGPACEDLSFGVIDLVRQTGVNISYDGGDCKGD
                     VQAGFVRGTKDNALYVKIIRGSKTLRLYKVKTGY"
     gene            complement(10743..11180)
                     /locus_tag="RHW33_003767"
     CDS             complement(10743..11180)
                     /locus_tag="RHW33_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647226.1"
                     /translation="MKTLIKILGITGSLLLCTEVQAQPYNRTLPAVVNDNNGNIFWCD
                     AHRQVTGKCEAMSVDRLGGMVTVRSAPFKDCEGGLWGWITIMRNDVSKGFTITPDKKF
                     GLTVKDLCRPGTVVTFKPNQKKPEYDDLVALYQGKELFRYRRF"
     gene            complement(11222..11758)
                     /locus_tag="RHW33_003768"
     CDS             complement(11222..11758)
                     /locus_tag="RHW33_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HDV1647227.1"
                     /translation="MTWHYEKNGIRHDNVTEDDITGLIKRGELTAATLVWRQGMPDWQ
                     PVSATPLASALLHSTIPPALPGSRIPGGVVWTLAFAPFIGYALELWMAGLNGMSFDEA
                     YEAVSGGQYWFIMLLLNIALGYLDERRLRKAGVDTTTFGKLAWLVPFYLWRRAKALGQ
                     KPAYFWVWLVMLGLTVGA"
     gene            complement(11784..12494)
                     /locus_tag="RHW33_003769"
     CDS             complement(11784..12494)
                     /locus_tag="RHW33_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV1647228.1"
                     /translation="MTQAERRHDRLAVRLSLIISRLVAGETLNMARLAAEFGVSVRTL
                     RRDFRERLMYLDLEYRRGQCRLRSAGGGVQGELDALTFAHRAGLADIFPGLDRRLAGM
                     LLTAGGMPCLVWQPPQSVSPAISLVFYRLVSAITACQRVLLLAEGERCDGLAPYRLIS
                     LDGCWYLTGELNGHITVHPLATIHAVTVLNTTFTPRKRLSHLTTQAGFIRALPHFSCI
                     REVLSPGPSEEKPDNTLI"
     gene            complement(12703..13527)
                     /locus_tag="RHW33_003770"
     CDS             complement(12703..13527)
                     /locus_tag="RHW33_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1647229.1"
                     /translation="MTRLASRFGAANLIRRDRPLTREELFRVVPSVFSEEKHESRSER
                     YTYIPTISLLESLQREGFQPFLACQTRVRDLGRREHTKHMLRLRREGQITGKQVPEII
                     LLNSHDGTSSYQMLPGLFRAVCQNGLVCGESFGEVRVPHKGDVVSQVIEGAYEVLGIF
                     DRVEEKRDAMQSLILPPPAQQALAQAALTYRFGEDHQPVTAPQILSPRRWQDESNDLW
                     TTYQRIQENLIKGGLSGRSAKGGRTHTRAVRGIDGDVKLNRALWVMAEAMLGGFQS"
     gene            complement(13621..14484)
                     /locus_tag="RHW33_003771"
     CDS             complement(13621..14484)
                     /locus_tag="RHW33_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV1647230.1"
                     /translation="MKKPEGLQSLQNSLSSLPQWVSERILHQVSQLTGYEPVIGIMGK
                     TGAGKSSLCNALFASEVSPVSDVAACTREPLRFRLQVGERVMSIVDLPGVGESQTRDA
                     EYATLYRQQLPQFDLVLWLIKADDRALAVDEHFYRQVIGEQYRHKVLFVISQADKVEP
                     ISCGDKLSTEQKQNISRKICQLHELFQPVNPVCAVSVRLQWGLRVMAERMIRCLPREA
                     SSPVVVQLSEPLRTDAVNKKARDDFGETVGSVLDTVSSLPLIPAPVRTIIQAVRDTVV
                     SVARAVWNFFF"
BASE COUNT         3870 a         3486 c         3782 g         3473 t
ORIGIN      
        1 aaataaatgg ctatttttac gaagagataa taacgtagat tgaaacaata tgtattattg
       61 attgagtaaa cgggattatt cttcacacta catccatttt agtgtcacta ccaaagaaaa
      121 aggggctacg ttttcatgta accccttgat ttatttggtg ggctggcagt gactgaattg
      181 gtgctgtaat tggttgaaat atttcatcta atgtctggtt caactttcct tagctgcctg
      241 aaagtttttt gatcttaccc agcaataaca gcgttacggt gcaccacgcc tgactgaaga
      301 actgcgtgct cagggttacc acttcaacgt aaaaccgtgg caggaggtcc ggactgaaaa
      361 aactgctcag gacatcatcg gcagggaacg cactgctagt ctgtaaactg aaccgactgg
      421 ggcgcagtat gtggcatctt gtcgtgctgt tggaggagct gtgcgaacgt ggtattaact
      481 ttcgtgctct ggcccaatct atatttgccc aacaatgggg ggacgaatgc tgtaaaagta
      541 aaacaatctg cgatctcaaa gttattgtgt gatttttatg tgagcagaag atattcatca
      601 gaaacgatta tgtaaatcat tttattttgc caacggcctc attgtcgaaa gaaaagaata
      661 cgcccgtgtt tcctgaatag attgactttt ttatccaacc acacttcagc gcactgcgtt
      721 taaaaaatgc ctcattctta tgcggaatat catcatttca tcatgatgtc tttgatgagc
      781 ggtgaacaca atacacttgc gctgtctctt caggatgaat cccctggtct ggtgcctgtg
      841 ggctgatgtt gcagcaaagc taaggtcgct taaacgctac tcagtattca cttttcagag
      901 gatgaaattt atgaacagga ccagtcccta ttattgtcgc cgctcagtac tttccttatt
      961 gatatctgcc ttgatatatg ccccgcccgg gatggctgcc ttcactacta atgttattgg
     1021 tgtggtaaac gatgagactg tagatggtgt ccaaaaagta gatgaacgag gtacaacaaa
     1081 taacactcat attatcaacc atggccggca ggaggtctat ggtgggatat ctaatagtag
     1141 tattattgaa actggtggcg agcaactagt atcaatccat gccgatatta acgggccagc
     1201 taataataca accattaatg gaggcagaca gtcaattgag tacggaggaa tttctactgg
     1261 cacgataatc gaaagtggca atcaatatgt tcataaaggt ggtaccagca atgacacgac
     1321 aattaagggc ggtacttcac gcatagaggg ggggactgca aatggaacaa tcattgatgg
     1381 tggtagccaa caagtaacaa ctcaggggca tgttgacggt acaacgataa ataagtctgg
     1441 ctctcaggac gtagtacaag gaagtctggc aacgaacaca accataaatg gtggtcggca
     1501 gtatattgaa cagagcacag tagaaacaac caccattaaa aatggcggtg agcaaagagt
     1561 atatgagagc cgtgcgctgg acactacgat tgaaggcgga actcagtctc tgaatagtaa
     1621 gtcaacggca aaaaatacgc agatctattc tggtggtacg caaattgttg ataacacgag
     1681 ctcctcggat gttattgaag tttattccgg cggcgtgctt gatgttagag gtggtacggc
     1741 aactaatatt acgcagcacg atggagccgc tttaaaagta acgacttacg atttgacggt
     1801 gagcggtacg aatagtgaag gggcattctc catccacaat aaagtggctg acaatgtgtt
     1861 gctggaaaac ggaggccatt tagaagtatc tcattctgca aacaagacga ttatcaaaga
     1921 taaaggaaca atgtcagttt taaccaatgc taaagctgat gcgacccgaa tagataatgg
     1981 cggggttatg gatgttgcag gaaacgcgac aaataccata attaatggtg gcacacagaa
     2041 tattaataat tatggcatag ccacaggcac caatatcaac agcggaacgc aaaatatcaa
     2101 aagcggcggg aaagctgaca caacaattat atcctccggg agccggcagg ttgttgagaa
     2161 agatggtacg gcaattggca gcaatattag cgccggaggc tcgctgattg tctataccgg
     2221 cggtattgca catggggtta accaggagac gggcagtgct ttagttgcca acacgggtgc
     2281 agggactgat atcgaaggat acaacaagct ctctcacttc actattaccg gaggggaggc
     2341 taattatgtt gtgctggaaa ataccggcga actgacggta gtggctaaaa cctcggcgaa
     2401 aaatactacc attgatgctg gcggtaagct gattgtccag caggaggcta aaacagatag
     2461 caccagactt aataatggcg gcgttctgga ggttcaggac ggtggtgagg ctaagcatgt
     2521 tgagcaacaa tccggcggcg cattaattgc ttccacgacc tccggaacac ttatcgaagg
     2581 aaccaacagt tatggtgatg ctttctacat caggaattca gaagctaaaa atgtagtgct
     2641 ggaaaacgct ggctcattaa cagtcgtcac tggttcccgg gcagttgaca cgattattaa
     2701 tgccaacggc aaaatggatg tttatggaaa agatgttggc actgtactca atagtgctgg
     2761 cacccaaaca atatatgcca gtgccacttc tgataaagca aatatcaaag gtggcaagca
     2821 aacggtatat ggtttagcca ctgaagcaaa tatcgaaagt ggtgaacaaa ttgttgatgg
     2881 tgggtcaaca gagaaaacac acatcaatgg tggcacgcaa accgttcaga attatggtaa
     2941 ggcaatcaat accgatatcg tctctggcct acaacaaatt atggcaaacg ggacagcgga
     3001 aggttccatt attaatgggg gttcacaggt agttaatgag ggcggtctgg ctgaaaactc
     3061 ggtgcttaat gacggcggca cactcgatgt gcgggagaaa ggcagcgcaa cggggataca
     3121 gcagagtagc cagggcgctt tggttgcaac caccagggcg acgcgggtca caggaacacg
     3181 cgcggatggc gtcgcgttca gcatcgagca gggtgcggcg aacaatattc tgctggcaaa
     3241 tggcggcgtg ttaaccgtgg agtcagacac ctcttctgac aaaacacagg tcaatacggg
     3301 cggacgggag atcgtcaaaa caaaagccac tgcgacaggc acgacgctca ccggcggtga
     3361 acaaatcgtc gagggtgtgg cgaatgagac aacgattaac gacggcggaa tacaaacagt
     3421 ttcagctaac ggagaggcaa taaaaacaaa gatcaatgaa ggcggtacgc tgacagtcaa
     3481 cgataatggc aaagcgacag atatcgtcca gaacagcggt gccgctctcc agacgagcac
     3541 agctaacggt attgaaatca gcggtactca ccagtacggc accttttcca ttgccggcaa
     3601 tttagcgacc aatatgttgc tggaaaatgg tggtaattta ttggtattag caggtaccga
     3661 agcccatgac tccacggttg gcaagggtgg tgcaatgcaa aacctgggtc aggactccgc
     3721 tacaaaggtt aactctggcg ggcaatatac ccttgggcgg tcaaaagatg agtttcagcc
     3781 tctggcccgg gcagaagatc tccaggtcgc tggcgggacg gcaatcgtct acgcaggtac
     3841 gctggcggat gcatcggtca gtggcgcgac aggaagcctg tcgttaatga cgccacggga
     3901 taatgttacg ccagttaaac tcgaaggggc ggtccggatt accgatagcg cgacattaac
     3961 tctgggaaat ggcgtcgata caacgcttgc cgacctgacg gctgccagcc ggggcagtgt
     4021 ctggcttaac agcaataatt cctgtgcagg tcccagcaac tgcgaatata gagtaaacag
     4081 tttgctactc aacgacggtg atgtttattt atcagcgcct gccacaacta acggtatcta
     4141 caacactctg acaaccaatg aactttccgg cagcggtaac ttctacctgc ataccaacgt
     4201 tgcaggctcc cgcggtgatc aactggtcgt caacaacaac gccactggta attttaaaat
     4261 ctttgttcag gataccggcg tcagcccaca gtctgacgac gcgatgacgc tggtgaaaac
     4321 agggggaggg gatgcttcgt ttacgctggg caataccggc ggtttcgttg atcttgggac
     4381 ctatgagtat gtcctgaaaa gcgatggcaa cagcaactgg aacctgacca atgatgtcaa
     4441 acccaacccg gatcccaacc caaatcccaa cccaaatccg aagccagatc caaaaccaga
     4501 cccaaaaccg gatccgaaac cagacccgac tcccgatcca acgccgacac ccgttccgga
     4561 gaaacgcatc acgccttcta cggcagccgt actcaatatg gctgcaacat taccgttggt
     4621 atttgatgct gagctaaaca gtattcgcga gcggttgaac ataatgaaag cgagtccaca
     4681 caacaataat gtctgggggg cgacgtataa cacccgtaat aatgtcacca ccgatgcggg
     4741 ggccgggttt gagcagacgc tgaccggaat gacagtgggg atcgacagcc gtaatgatat
     4801 tcctgagggg attgcgacgc tgggcgcttt tatgggttat tcccattcac atatcggttt
     4861 tgatcgtgga ggacatggca gtgtgggcag ttattctctg ggcggctatg ccagttggga
     4921 acatgaaagt ggtttctatc tggacggtgt cgtgaagctg aaccgttttg aaagtaacgt
     4981 agccggtaaa atgagcagcg gtggagccgc caatggcagt taccacagca acgggctggg
     5041 cggtcacatt gaaaccggga tgcgatttac cgatggtaac tggaacctga cgccgtatgc
     5101 atcgttaacg gggttcaccg ctgataaccc cgaatatcat ttatccaatg gcatgaaatc
     5161 gaaatcagtc gatacccgca gtatatatcg tgaactgggc gcaacgctga gttacaacat
     5221 gcgtctggga aacggtatgg aaattgagcc gtggctgaag gcggctgtgc gcaaagaatt
     5281 tgtcgatgat aaccgggtga aggtgaataa tgacggtaat ttcgtcaatg atttgtcggg
     5341 cagacgtgga atataccagg caggtattaa agcctcattc agcagtacgc taagcgggca
     5401 tctcggggtg gggtatagca acggtgccga tgtggaatcc ccgtggaacg cggtggctgg
     5461 tgtgaactgg tcgttctgac catcaacgaa aaagcccaca tctgtgggct ttcatgtcac
     5521 caggagccgc ggctcctttg cgtatccttt tatgtctcct caccgtctgg tcggtgtcct
     5581 gctaagactt ctaacttcct gtttttgttg gtgttgtcct tacaccgtcc aatcatgatt
     5641 ggtgggctgg cggagtctga attggttttc taaccaattg aattaaatcg ttaatttatc
     5701 aattcaactt ttcattggtg cctaagaggg tgcctaaaac atttttgcat tatttcggct
     5761 cgtttttcat cacctaaaaa cgctggttag tagaaggtgg gagttggtcc gggtaatgct
     5821 tagcgatgat gtcgttcatc ttctctattg acgcagggcg cttgaacgtg agatgagcaa
     5881 ttttcttttg aaagtagcga atttcgcttt accattgagc aaaaaacaag cgttctgcga
     5941 gttggtattg ttacttttac ggctgtattt tattctgcat ttgcttcttt aatcctgtag
     6001 ggacgtaaca ttaccaacca gaatcttttg accccaacac agagtcagca tcgctatctg
     6061 cggtgtctcg gcaatggtat aaacaaaaca gtgcttacgc catcaagcct tcgctccagt
     6121 aagactaacc ctttcagcaa agttccccag ctcaccagca catctgtccg catgttgttc
     6181 ttcttaccga atgagaaaat gtacacaaga tgcagtatga tgaggcatgt agtgtgttgt
     6241 ctagttatgc cgcttttaat gtggagctga gcttttgaaa atcaatcata ttgaagttag
     6301 taattttaga aagtacaaag ctgcaaggtt cagctttcat ccagaattta cggttctgat
     6361 tggcaataac gcatcaggga aaacaaccat actggatgcc atagcgttgt tactgaatac
     6421 ctactttcag ggatcaggtt tgactaccgg tggtgggacg ttaaaaaaag acgatgcccg
     6481 ctatatccat acccgaaaag aagaacaaga ctttcttgaa cctcagtcac ccgtctggtt
     6541 aaagggggtt gccgcgcttg cgggagagac gtgtgaatgg gtaagagaaa tgggggatcg
     6601 aggtggaaag gctaaagctt tcacggctta tggggcactt gaacgtgaag gtgttgccaa
     6661 aggtcaaagt ccctcgctgc ctatgctgct ttattatggt gccggacgtc tgtgggattt
     6721 acatcgtgat aacaaaatgg atacaacagg ttctcaattg ggtgcttatc gctactgcct
     6781 ggaccccaaa tcagatcagt attcatttat aaaatggttt atcaaactga cgatgacaga
     6841 actgcagcgc aaaacgccag tacctgcatt acgtgctgtt gaaagagctg ttttgcaatg
     6901 tgctccgggt gcaaaatcct tttattacga cgtaggctca gagactatct taatagaact
     6961 caaaaaggaa ggattggttc gatttagcga tttgagtgac ggataccgta atatggttgc
     7021 tatgatcgca gatattgcac accggtgtgc ccgattaaac cctcagatgg gggaaaatgt
     7081 tactttcgaa acgaagggta tcattctcat tgatgaactc gatcttcatt tgcatcctaa
     7141 gtggcaaaga cgtgtcgtac ccgatttacg taaaacattc cccaacatcc agtttattac
     7201 gactagtcat tcgcccttca ttatccagtc gatgaacccg ggggaaatta ttgatcttga
     7261 taacatacta gcagatgaac aacctcagtc tgttccagaa ggtattgctt caccagcacc
     7321 cagtatgtcg ttcagcgcaa aatccataga agatattgta gaaaatgtaa tggatgttcc
     7381 gctaccccag cgtagtcagc gttatcaaga aatgcatgat gttgcagtaa aatattacca
     7441 gctacttgaa caaggtcaaa atgctgaagg ggcagaggtt cttcgtctta aggatgagct
     7501 ggatgcgctg tcggcaccct ttagtgataa cgttgcatat catgcctttc tggagatgga
     7561 acgagcagca gcgggcctag gcaggtctcg tggagaagat gaataatgcg accaatcgaa
     7621 cgaggaggat gcccggttga taataatcag cagaaaatcg ttttttcgca ttattctgat
     7681 gcaagaggag aactgattaa aagaatgggg cagtattgtt cctactgtga aatgaagcta
     7741 gatgcctcac tggccgttga acatgtacag ccaaaaaaag cccgtggcag taatgtggtg
     7801 aatcaaacac tgttactgga ctgggagaat tttttactcg catgcactaa ctgtaattcg
     7861 acaaaaggta ataagccagt tgtcctaacg gattatattt ggcctgatac agataatacc
     7921 ttcagagcgt ttgtctattc tactggtgga atcatctcag cagccccagg accagtgcac
     7981 caggaagcta aaaatatgat tgctttggtc gggctcgata aaaaacccga taccgcggat
     8041 gcttccgata ggcgatggtc taacagactt gaaacttggg gcatggctga acgcgccaga
     8101 acgcgcttga taaatcgcga ctctattgaa cttcgtgaaa caatagcgga acatgcttat
     8161 tcccaaggat actggagtat ctggatgacg gtattcagtc atgatagtga tatgctgcga
     8221 aggataatcg cgcaatatat gggaacaagt gctttgtgct tcaataatac tgggatggct
     8281 cttcccaggc cacaagggaa atgctgatga ggccaattac taatatgctg cggaatgacc
     8341 gactcgacac agaacgatgt atttagctgc aatctgactg cttttccagc aggtacacaa
     8401 tcagtgtttt tcagtgcgct ttgggtaaaa ggcagcatga tggcgcccac cttggatgaa
     8461 ttgctgaggt tgtataattg tgttctttcg gcgggttcta cagaggtgtg aggtaaatcc
     8521 tgctatggat gtaactatgc gaaccgatcg taccggcttg acgctctgtt cgttgagttc
     8581 aatagtgacg cagtgctgct agcgtggatt cagttcacaa gaagtcaaca ttaattccat
     8641 ttggctggct aggtgtgaga atgtttcatt caattcaata tgttcggcgt cactgaattt
     8701 acctgtgata ttggcccggt cacccagata tagcgtgaaa tcccgttgta acctccatgt
     8761 aaagttactt ctcatcgggc ttctccttta ttgtcagtac agggggacac tcatcagaca
     8821 ggcatagggc ccatagtggt cctggcggcg ttcggtgctc accgccagta ctccgggaat
     8881 gtcaggaact acctaatcgg tgtaatgaca aacacctcaa agcagaccat gttcagcgaa
     8941 cgacaatatc tgcgtaccac cgacgataag gtggtcaggc acacggatat caacgagctg
     9001 aagcgcctgt accagacgct gcgttatggt cttatctgcc tggctggggg tagtgtcgcc
     9061 ggagggatgg ttatgcgcca atattaccgc cgccgcattg aagtacaggg cgcttttgac
     9121 cacctcacga ggatgtacct cggtactgct aatagaaccg gcaaacaggg tttcgtgggc
     9181 aatcaaccgg ttctgctggt tcagatacag caccataaac tcttcgcgct ccagtcccgc
     9241 cattttcagt ttcagccagt cacgggcagc ctgagtggaa gtgaatgcta cgcctgtttc
     9301 gcgcaggtgt cggtcaagca gcgttaaggc gcgttttacc gtgcgttgtg ctgttagtgg
     9361 tagtaccgga gtcagtggga taagtgactg ttccatcatg agtatcctta atcaataatg
     9421 cgcagaatgg cgctggattc agggtgctgc agggcatagt cccgcaagcg ataatagtgt
     9481 gcggtcattg cgtcacattc tgtacgacag gcgtgatggc tgtattcaat caggcaaaca
     9541 gcgatacctg cggcttctgc gctcatttcc gcaccattgc cgttcatgcc gttgtgcaga
     9601 tgccatttat cgtcactgtc agcatcagga gccataaacg cgccgccatt actgagcgtg
     9661 taaaaggacc agataccgcc gttgtactca tcacagaagc ggtccatcca ggcgaaaata
     9721 cggggttcca gcgataccca ctgcgggata tcgccaaagt acagcggcca gaaatcgaga
     9781 cgctgttcgt caggcactgg tgttgcagtg aggtcaaatt gaggttcatt agcggacatc
     9841 aattcgcatt gagtttgtgt cgtcatggat attctccgtc aataaaaacg ccagcaacga
     9901 tggctggcat atgggaaata caggatatgc agggatggtg aatgggtgtg atcagcagga
     9961 ggatgctctg agcatgcctg acttgcggat ataacgatta agtccttcgc cagcatcagg
    10021 ttcaaagcac catgcccgcc agaccattcg accttcagag tcacgaacca ccaggcggaa
    10081 gtggctaccc tggtcgtctt caatagcgat attttgatac cggtgggtaa cagcttcagc
    10141 ctgctgtcgg gtgaaagatc ccggtggcag catgtgagat tcagacattc ggtttacctt
    10201 aaaagcaaaa gcccctgacg cagatgcgtc aggggctgtg ggtgtatgca ggtgtggggg
    10261 gattatcagt aaccggtttt caccttatac agccgcaggg ttttgctgcc ccggataatt
    10321 ttcacgtaca gggcattatc tttcgtacca cgcacaaacc cggcctgcac atcgccctta
    10381 cagtcaccac cgtcgtaaga aatgttgacg ccggtctggc gcaccaggtc gataaccccg
    10441 aagcttaggt cctcacaggc cgggcccggc aggtcctgaa tcagggcaaa tttatggtcg
    10501 tcgctgacac cggtcagtga gcacatggag gtattgatgt cgtttccctg gcaccagtac
    10561 tcctggccgg gattgtcttt atccatcagg cttgccggaa gttgagcggc accggcactg
    10621 aatgcagcaa gtgacagaat gagggcgggt acagcagagg tcatcatttt cagcatggtt
    10681 ttcttcctga attaatccag cccgctgtcg ggcaaaatga gagagattga tatcgggtgt
    10741 tatcagaaac gccggtagcg aaacagctct ttgccctggt acaacgccac caagtcgtca
    10801 tattccggct tcttctggtt gggcttaaag gtcactaccg tgcccggacg gcacaggtct
    10861 tttacggtga gaccgaattt cttgtccgga gtaatggtga atcccttcga tacatcgttg
    10921 cgcatgatag ttatccagcc ccacaggcca ccttcgcagt ccttaaaggg tgctgaacga
    10981 actgttacca tcccacccaa gcggtcaaca gacatggctt cacattttcc ggtaacctga
    11041 cgatgtgcat cacaccagaa aatattcccg ttgttgtcat tgacgacagc aggtagcgtc
    11101 cggttataag gctgggcctg aacttctgta cagagcagaa ggctgccagt aatacccagt
    11161 attttaatca gtgttttcat tacgttggtc cttaacagag aaagtctcag gtagcgatga
    11221 ttcaggcccc gacggtcagg cccagcatca ccagccatac ccagaagtaa gccggtttct
    11281 gaccaagggc tttcgcccgt cgccacagat agaacggcac cagccaggcc agtttgccga
    11341 acgtggtggt gtccactccg gccttacgca ggcgtcgttc atcgagatac cccagagcga
    11401 tattgagcag cagcatgata aaccagtact gcccacccga gacggcctca tatgcctcgt
    11461 caaaagacat tccgttcagc cctgccatcc acagctcgag cgcataacca atgaaaggag
    11521 caaatgccag cgtccatacc actccgcccg gaatgcgact ccccggcagc gctggtggta
    11581 tggtgctgtg gagcagcgcc gaagccagcg gggtggcgga gaccggttgc caatccggca
    11641 tgccctgtcg ccacaccagc gtggcagcag tgagttcacc gcgcttaatc aggccagtga
    11701 tgtcgtcttc ggtcacattg tcgtgacgta tgccattttt ctcgtaatgc caggtcattg
    11761 cttttccttc ttaacagagg ttattaaatc agtgtgttgt ccggcttctc ctcagaggga
    11821 ccaggagaca ggacttcacg gatgcagctg aagtgcggaa gggcccggat aaagcccgcc
    11881 tgtgtggtta aatggctgag acgttttcgc ggggtaaaag tggtgttcag aactgtcacc
    11941 gcatggatag tcgccagagg atgcacggtg atatgcccgt taagttcacc ggtgagatac
    12001 cagcagccat cgagtgatat caggcggtat ggggccagcc cgtcacagcg ttctccctcg
    12061 gccagcagca gcacccgctg acaggcggtt atggcactga cgagacggta gaacaccagt
    12121 gagatggccg gggaaacaga ctgtggcggc tgccatacca gacacggcat ccctcctgca
    12181 gtgagcagca tgcccgccag acgccggtcc agccccggaa aaatatcggc cagtccggcc
    12241 cggtgagcaa aggtcagcgc atccagctca ccctgtacgc ctccaccggc actgcgcagg
    12301 cggcattgcc cccgtcgata ctcaaggtcg aggtacatca gacgttcgcg aaaatcccgt
    12361 cgcagggtac ggactgacac accaaactct gcagccagcc gcgccatgtt cagcgtttca
    12421 cctgcaacca ggcggctgat tatcagtgac agcctgacag ccagccggtc atgacggcgc
    12481 tctgcctgtg tcatcttaaa tctccgtaat agttaactga ctgaatatga aatgattcta
    12541 aagagggggg cggacagggt atggacatgg aatgaactat tttttatctg cctccggaag
    12601 caggcccgac gcggcacacc gtgaagtgcg gtgaaaaaac gctcaatgca atggaggcaa
    12661 aacggacagg gagtgtcctt ttctaacatg ctgataacaa gctcaggact gaaacccacc
    12721 cagcatcgct tcagccatca cccagagagc tcggttgagc ttcacatctc cgtcgatacc
    12781 acgcacggcg cgggtatgcg ttcgcccgcc ttttgcactc cgaccactga gcccgccctt
    12841 aatcaggttt tcctgaatac gctggtatgt ggtccacaga tcattgctct catcctgcca
    12901 gcggcgtggg gagagtatct gaggtgctgt caccggctgg tggtcctcac cgaaacggta
    12961 cgtcagtgca gcctgtgcca gagcctgttg tgcaggtggc ggcagtatca gcgactgcat
    13021 ggcatcccgc ttctcttcca cacggtcaaa aatacccagc acttcatacg ccccctcaat
    13081 cacctgactc accacatccc ctttatgcgg cacccgcact tcaccaaacg attcgccgca
    13141 gacgagcccg ttctgacata ccgcacgaaa taatcccggc agcatctgat acgagctggt
    13201 gccatcatgg gagttgagca gaatgatttc tggcacctgt ttaccagtaa tttgcccctc
    13261 acggcgtagg cgcagcatgt gctttgtatg ttcgcgacgc cctaggtcac gaactctggt
    13321 ctgacaggca aggaacggct gaaagccttc ccgctgcagg ctttccagca gggagatggt
    13381 ggggatgtat gtgtaacgtt cactgcggga ttcgtgtttt tcctcgctga acacactggg
    13441 caccacacga aacagctctt cacgggttaa cggacgatca cgacgaataa gatttgctgc
    13501 gccaaagcgc gaagccagac gggtcataag cagactcctc atgatgggaa ataaagacaa
    13561 aaaagccctg ccgctcaaat ggcgacaggg ctcaggaaac gacggattaa gtggcaatat
    13621 tcagaagaag aaattccaga cggcacgggc aaccgacact acggtatcgc gtacagcctg
    13681 aatgatggtc cgaaccgggg ctggtatcag gggtagggaa cttactgtat ccagcaccga
    13741 gccgacggtt tcaccaaaat cgtcacgagc ttttttatta acagcatcag tacgaagtgg
    13801 ctcactgagc tggaccacca ccggactgct ggcctctcgc ggcagacaac gaatcatccg
    13861 ctcagccatc accctaagcc cccattgcaa acgaaccgat accgcacaga ccggatttac
    13921 aggctgaaaa agttcatgca actggcagat tttacggctg atgttttgtt tctgttccgt
    13981 ggacaactta tcaccgcagc tgattggctc gaccttgtcg gcctgactga tgacaaacag
    14041 caccttatgc cggtactgct cgccaatcac ctgacggtaa aaatgctcat ctaccgccag
    14101 cgcccggtca tcggccttaa tcaaccacag cactaggtca aactggggaa gctgctgccg
    14161 gtacaatgtg gcatactctg catcacgggt ctggctttcc ccgacaccag gcagatccac
    14221 gatgctcatc acgcgttccc ccacctgcag tcgaaagcgc agtggttcgc gagtacaggc
    14281 agccacatcg ctgacaggtg aaacctcact ggcaaacagg gcattgcaca ggctgctctt
    14341 tcccgccccg gttttaccca taatgccgat cactggctca tagccggtta actggcttac
    14401 ctggtgcaga atacgttcag acacccattg cggcaagctg gaaagtgagt tctgcagcga
    14461 ctgcagacct tcaggttttt tcatcactac tcctctgaaa tgaaaacaaa aaaggcagaa
    14521 ccgtgaggtt ctgcctttaa tgaggtatgt tcagttgaat gatatatatc tgaatatttt
    14581 tacaatgctg ataggccgtt ctgtgccagg a
//
LOCUS       DAPGRN010000115        14582 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPGRN010000115 DAPGRN010000000
VERSION     DAPGRN010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.117"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1856
                     /locus_tag="RHW33_003772"
     CDS             <1..1856
                     /locus_tag="RHW33_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1647231.1"
                     /translation="TEQLQQFIKIMEQKAQLPIYIDKNNLKESIFSVLHNDPQHVDKD
                     QHLPCDKFLKHACKSSNSFEVKLDATHQYQQLNNFMISFDPVENQLTIRDNNNKTETF
                     SFTNLQWENLLQYYKENHQQPNIAGSRNLTDNIDKIKNTISTSEIIECASPEIRSSVL
                     NDLYSIANFLPDNNLTPNESWKRFCETCERFYVAQKSITGDKSERLTRKLSISDAGIT
                     MTFTIGDVVINTISTAIPEDSTGQRCIEGLNLAEMDLTGIDLSKMVLRNVNFNGSILR
                     HAKFSGTICEGVDFTDCDLRNAKFKNASLENNDFRKVRHLTYVNFKNANLRNSNFSGK
                     VLTGVNFTGSNLSNAYLEYIDFTTVILYETPATPATSETAKIPEIPGTHKIPGTHKIP
                     ATPKIPVTLQIPETPKVILTGAILNYSDLSGKDLSEYNLTGIICMYTNFSNTNLTNCE
                     LSNANFSNAKFYNTNCTGANCSDILFDYAWFDNTIFIDAIFKNVCFYNVRAKNVSLEG
                     AYLNNDNIAKQANNSTEKQANDSIDKQANDSTVQQSIDSTVQQANDSTDKQANDNIDK
                     QVNDSTDKQAKNSTKQQDSNSFNQARLKKEVNRRFSIPGLTSYQPTYIVEE"
     gene            2126..2968
                     /locus_tag="RHW33_003773"
     CDS             2126..2968
                     /locus_tag="RHW33_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313321.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV1647232.1"
                     /translation="MKKRITFIVFLCAIVAVSLFFVQSCVRKSQHVAGFQNYQATIDG
                     KEITGVTKNISSLTWSAQSNTLFSTINKPATIVEMTTEGDLIRTIPLDFVKDLETIEY
                     IGDNKFVISDERDYAIYVISLNADSEVSILKKIKIPLQETPTNCGFEGLAYSPQDHTF
                     WFFKEKNPIEVYKVTGLLRSDELHISKDKALQRQFTLDDVSGAEFNPQKNTLLVLSHE
                     SRALQEVTVIGDVIGEMSLTKGKYGLSHNIKQAEGIAMDDSGNIYIVGEPNLFYRFTS
                     TKSR"
     gene            3036..4214
                     /locus_tag="RHW33_003774"
     CDS             3036..4214
                     /locus_tag="RHW33_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647233.1"
                     /translation="MNSTSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMLAE
                     GEFSFSELSWIASGNYAGYLAGSLLFSFGAFHLPSRLRPFLLASALATGLLILAMAWL
                     PPFLLVFIIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGVGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSAIILLALALLIPSNKHVIPPAPLAKIAQQPMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGQPVLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASSSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALICGMQIFKLHTNYS
                     "
     gene            4425..9665
                     /locus_tag="RHW33_003775"
     CDS             4425..9665
                     /locus_tag="RHW33_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HDV1647234.1"
                     /translation="MASTNAHQIKNNVQNSLCGLGDKIRRLTAGVCLFTQIFFPVMAT
                     AQNVVHAKPQTTVSSAPPLIENNTVPYTLGALESAQSVADRFGISLEELRRLNQFRTF
                     ARGFDNVRQGEELDVPATTSQKSHEQQNAVPPANGENTLENQIASTSQRVGTLLSQDM
                     NSEQASGMARGWASSEASGAMTDWLNNFGTARISLGVDEDFSLKNSQFDFLHPWYDTP
                     DYLLFSQHTLHRTDDRTQINTGLGWRHFTSSWMSGINLFFDHDLSRYHSRAGLGAEYW
                     RDYLKLSSNAYIGLTGWRSAPELDYDFEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFDKNDRQSNPHAITAGLNYTPFPLLTLSAEQRQGKQGENDTRFAVDLTWQP
                     SSSMQKQLNPDEVAGRRSLAGSRYDLIDRNNNIVLEYRKKELIRLSLLDPVKGKSGEI
                     KPLVSSLQTKYALKGYNIEAAALEAAGGKVSTSGKDITVTLPGYRFTNTPETDNTWSI
                     DVTAEDVKGNLSRHEQSMVVIQAPTLSQKDSLLSVNPLTVAADKKSTTTLTVTAHDSD
                     GTPVPGLALQTRSEGVQDITLSDWTDNGDGSYIQMLTAGTTSGSVTLTPQINGESAVK
                     ESIVVNIVPVVSSRDHSSITIDNVSYYAGDDIKVRVELKDDSNQPVAYQKEELVKAVT
                     VENSKPGATIVWHEEQPGVYAANYPAHKQGTALRAQLSLHNWNAPLQSHIYNIEANQN
                     MARVATLSATNNDVYADKKTFNTLTINVTDESDNPLINHQVTFKNEKGSAEFVEPPQQ
                     NTDGYGVATINMVSQVAEENTISATLPNGFSQRIIAKFVSDSSTPKFKQLVADPDTII
                     AGNSQGSTLTAIVTDFHNNPLKDMKVNFVAPGGSQLDNTTATTDQSGIVRVHLTSSKA
                     GSYSVDASLEVDKNIHQSVTITVVPNREQSVMTLNAGSGSAIANNTNIVTLTASVKDV
                     YGHPLPDEDVKFTLPASMTGNFTLSSETARTDANGDAVVTLRGTKAGEFTVTATLTRN
                     NTVVYQQVTFIGDTNSAQLQPLTASLNSIVAGNSTGSTLTATILDAYQNPLKDQLVTF
                     QSNDVTLSGTEVTTNTLGQATVTMTSNIAGQHNVVVSRKAQASDNKTFSLSVLPDESS
                     AKVISITGAEKTITVGENITLRILVQDAFNNVIAGQRVRLSAQPTTNITIGDTAYTDN
                     NGYAYVNLLSTQPGVYQVTATLDNNSSSKVDVNVANGKLELTSSKPETTVHNSEGITL
                     TATARNARGELMPGQIITFSVTPEGATLSNTGEVLTDQSGQAKVTLTSDKVNVYTVTA
                     IMGKDVPVQSQVTVAVKADAKTAHVVSVVASPDTITADGIDSSTITSRVEDDYGFPVE
                     GVDVSYTLDTKGRPVVNIPTTRTDQSGQVTATITSTLAETLTVNVQVPGTANQSAAIT
                     LIAGTADESKSILKSDVSTLMADYQHSAKLTLTLQDKYGNPIVTSDHLEFVQSGPFVN
                     FLKLSDIDYSQRNYGEYTVTVTGGKEGTATLIPMLNGVHQANLSVSLNLIRSIKEMSG
                     HVTANNHTFSTAKFPSEGFAGAYYTLNNDNFEAGKTVDDYMFSSSQSWVSVDASGKVS
                     FANIGDQTSVTISAVPRQGGTTYQTLIKLKGWWVNNGNHTNIWLAANALCHAKNDGYN
                     LPSITHLTSGENKRTQGSLYGEWGNVGAFSSNSQFTQGAYWTSESDDYNRHYYVQMLT
                     GMTGSDADSSPQLTACRKSL"
     gene            complement(9789..10373)
                     /gene="kptA"
                     /locus_tag="RHW33_003776"
     CDS             complement(9789..10373)
                     /gene="kptA"
                     /locus_tag="RHW33_003776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV1647235.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            10623..11306
                     /locus_tag="RHW33_003777"
     CDS             10623..11306
                     /locus_tag="RHW33_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647236.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            11303..11764
                     /locus_tag="RHW33_003778"
     CDS             11303..11764
                     /locus_tag="RHW33_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV1647237.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            11777..12949
                     /gene="iadA"
                     /locus_tag="RHW33_003779"
     CDS             11777..12949
                     /gene="iadA"
                     /locus_tag="RHW33_003779"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV1647238.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            13014..13925
                     /gene="hypT"
                     /locus_tag="RHW33_003780"
                     /gene_synonym="qseD"
     CDS             13014..13925
                     /gene="hypT"
                     /locus_tag="RHW33_003780"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV1647239.1"
                     /translation="MDDCGEVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(13918..14310)
                     /gene="iraD"
                     /locus_tag="RHW33_003781"
     CDS             complement(13918..14310)
                     /gene="iraD"
                     /locus_tag="RHW33_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV1647240.1"
                     /translation="MMRQSLQAVLPKISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(14307..14390)
                     /gene="idlP"
                     /locus_tag="RHW33_003782"
     CDS             complement(14307..14390)
                     /gene="idlP"
                     /locus_tag="RHW33_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV1647241.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         3942 a         3564 c         3434 g         3642 t
ORIGIN      
        1 aaaccgagca attgcagcag tttattaaaa taatggaaca aaaagcgcaa ctaccaatct
       61 atattgacaa gaacaatttg aaagagagta ttttctctgt tttgcacaat gacccacaac
      121 acgtagataa agatcaacac cttccctgtg ataagttttt aaaacatgcc tgcaaaagtt
      181 caaattcatt tgaagtgaaa ttagatgcca ctcatcaata tcaacaactg aataacttca
      241 tgatttcttt tgacccagta gaaaatcaat taaccatacg ggataacaat aacaagactg
      301 aaactttctc gtttacaaac ttacaatggg aaaatttgct gcaatactac aaagaaaacc
      361 accagcaacc aaatatagca ggatcacgaa atctcacgga taatatagat aaaattaaaa
      421 atacaatatc cacctctgaa attattgaat gcgcctctcc tgaaataaga agtagcgtcc
      481 tgaacgatct ttatagcatt gctaatttcc tcccggacaa taatctgacc ccaaatgaga
      541 gctggaaaag attttgtgag acatgcgagc gcttttacgt tgctcagaag agtatcactg
      601 gagataagag tgaacgtctt acgcgaaaac tctctatctc tgacgcagga attacaatga
      661 ccttcacgat aggtgatgtt gtcatcaata ctattagcac tgctattcca gaagattcaa
      721 cggggcaacg gtgtatcgaa gggttgaatt tagcagaaat ggatttaacc ggcatagact
      781 tgtctaaaat ggttctaagg aatgtcaatt ttaatggcag cattcttaga catgccaagt
      841 tctccggtac gatctgtgaa ggcgtggatt ttacagattg tgatctgcgt aatgcaaaat
      901 ttaaaaatgc ctcattagaa aataatgatt ttcgtaaagt tcgccacttg acttatgtaa
      961 atttcaaaaa cgcaaatcta cgaaacagta acttcagcgg aaaagttctc actggcgtta
     1021 attttactgg aagtaacctt agcaacgctt atcttgaata tatagatttc acaacagtga
     1081 ttctatatga aacacctgca acacctgcaa catctgaaac tgctaaaata cctgaaatac
     1141 ctggaacaca taaaatacct ggaacacata aaatacctgc aacgcctaaa atacctgtaa
     1201 cacttcaaat acctgaaaca cctaaagtaa ttcttactgg agcaatacta aattattcag
     1261 atctatccgg gaaagatctt tcagaatata atcttactgg tattatctgc atgtatacca
     1321 atttttcaaa cactaatttg acaaattgtg aactctctaa tgcaaacttt tcgaatgcaa
     1381 aattctacaa tactaattgt actggtgcaa attgttcgga tatcctattt gactatgcat
     1441 ggtttgacaa tacgatattt atagatgcga tttttaaaaa tgtctgtttt tacaatgtca
     1501 gagcgaaaaa tgtctctctt gagggagcat atctgaacaa tgataatatc gcgaaacaag
     1561 ccaataatag taccgagaaa caagccaatg acagtatcga taaacaggcc aatgacagta
     1621 cggtgcaaca atccattgac agtacggtgc aacaagccaa tgacagtacc gataaacaag
     1681 ccaatgacaa tatcgataaa caggtcaatg acagtaccga taaacaagcc aagaacagta
     1741 ccaagcaaca ggacagtaac agttttaatc aagcccgttt aaagaaagaa gtgaatagga
     1801 gattttccat tccgggttta acgtcttatc agccaacata tatagttgaa gaatagttca
     1861 ttaatcaccc aaacagagtg ggtatggcag acgatataga ttaacattta aacgttaacc
     1921 gcctcatcta tcattcatgg atgaggcgca tttatcagtc gatgtaagct gaaaatgttc
     1981 tgccatccag agacaaccgc cgcccccctg cggcgtttac tatttcatcg cccgcgttaa
     2041 gaattcatta agcttcatcc gtatactcct tatctgtcgc actgaatttg acgagaaaaa
     2101 aaaagcaata ataaggagca acaagttgaa aaagcgcatc acctttatcg tttttctatg
     2161 cgccatcgtg gcggtgagcc tgttttttgt gcaatcctgc gtccgtaaaa gccagcatgt
     2221 cgcagggttt cagaattatc aggccactat cgatggtaaa gagattacag gagtaactaa
     2281 aaatatctcc tcactgacct ggtcagcgca gagcaatacc ttatttagca ctatcaacaa
     2341 acctgcaacc atcgttgaaa tgacgaccga gggcgacctg atccgcacca ttccgctcga
     2401 ttttgttaag gatctcgaga cgattgaata cattggtgat aataaatttg tcatcagtga
     2461 tgaacgtgat tacgccattt atgtgatttc acttaacgct gactcggaag tgagcatcct
     2521 caaaaaaatt aaaatcccat tgcaggaaac gcctactaac tgtggctttg aaggactggc
     2581 ttactcccct caggaccata ccttctggtt ttttaaagag aaaaatccga tagaggtcta
     2641 caaagtaacc gggctattgc gtagcgatga gttgcatatc agcaaagata aagcgttgca
     2701 gcgtcagttt actttagacg atgtttctgg cgcggaattt aacccacaga aaaatacgtt
     2761 gctggtgttg tcacatgagt cacgcgccct gcaggaagtg accgtgatag gtgacgtgat
     2821 cggcgaaatg tcgttaacca aaggtaagta tggcctttca cacaacatca agcaggcgga
     2881 agggatagcg atggatgata gcggcaacat ctatatcgtt ggcgaaccga atctttttta
     2941 tcgctttacg tcgacaaaat cacgataaca gtctggcagg ttctcctttt ttgttattaa
     3001 cttgttaact caaaataata caaaccaggc agaacatgaa ttcgacatcg catcccgtag
     3061 aacgtttttc tttcagcacc gcgttattcg ggatgctggt tctgacctta ggtatgggtt
     3121 taggccgctt tctctatacg ccgatgctgc cagtcatgct ggcggaaggc gagttttcat
     3181 ttagcgaact ctcatggatc gccagtggta actatgccgg gtatctggcg gggagcctgc
     3241 tgttttcatt cggcgcgttt catttaccct cacgcctgcg cccgttcctg ttagcttccg
     3301 ccctcgcaac cggattatta atcctcgcga tggcgtggct gccgccgttt cttctggttt
     3361 tcatcattcg ctttctggcg ggagtcgcca gcgccgggat gttgattttc ggctcaacgc
     3421 tcatcatgca gcatactcgt catccctttg tccttgcggc gctattttct ggtgttggcg
     3481 tcggcatcgc tctgggcaat gaatatgtgc tggcaggcct gcattttgcc ctctcttcac
     3541 aaacgttgtg gcaaggtgcc ggagcacttt ctgccattat attgcttgct ctggcgctgc
     3601 tcatcccgtc gaataaacac gttatcccgc cagcgccatt ggcaaaaatc gcgcaacaac
     3661 ccatgagctg gtggttactg gcgattctgt atggtctggc gggttttggt tatatcatcg
     3721 tcgccaccta cctgccgctc atggcgaaag acgcgggcca gcctgtgctg acggctcacc
     3781 tctggacact ggttggcttg tcgattgtcc caggttgctt tggctggctg tgggcagcca
     3841 aacggtgggg agcattacct tgcctgaccg cgaatttgct ggtgcaggcg atctgcgtgc
     3901 tgttaaccct cgccagcagc tctcctttat tactcatcat cagcagtatt ggttttggcg
     3961 gcacctttat gggaacgacc tcgctggtga tgaccatcgc ccgccagctt agcgtaccgg
     4021 gaaatcttaa ccttttgggc tttgtgacac tcatttatgg tatcgggcaa attcttggcc
     4081 cggcgctgac tagtatgctc ggcaatggaa cgtcggccct cgccagcgcc acgctctgcg
     4141 gcgcagcggc gctatttatc gcagcattaa tctgcgggat gcaaatattc aaattgcata
     4201 cgaattattc ttaagtaatt taacgaattt aacttttcag tacatcacgt aaaataatta
     4261 aaacatcctt tttctttgga ggtactcagc caccgccgtt tttgttcctg gtttagcaat
     4321 tccacggcca attcattatt tctggtcaca ttatagtcag gatcgcttta tatctccgtg
     4381 ctagaaaaaa caagtttaat tctgtttttt cacggaaggt cactatggct tcaacaaatg
     4441 cgcatcaaat aaaaaataat gttcaaaact ctttatgtgg tctgggtgac aaaatacgcc
     4501 gcctgaccgc cggagtttgt ttatttacac aaattttttt ccccgtcatg gcgacggcac
     4561 aaaatgtggt acatgctaaa cctcagacga cagtttcatc tgccccccct ctaatagaaa
     4621 ataatacggt gccttacacg cttggtgcgc tggaatcagc gcaaagcgtt gctgatcgct
     4681 tcggtatttc actggaggag cttcgtcgtc ttaatcagtt ccgtactttt gctcgcggct
     4741 ttgataacgt gcgccagggt gaagaactag atgttccggc aacaacctcg cagaaaagcc
     4801 atgagcaaca aaatgccgta ccgcctgcga atggcgaaaa cacgctggag aatcaaatag
     4861 ccagcacttc gcagcgagtt ggcactctgc tttcacaaga tatgaatagt gagcaggcca
     4921 gcggcatggc gcgtggttgg gcgtcttcag aagcctcagg cgcgatgact gattggttaa
     4981 ataactttgg tactgcaaga atctctctgg gtgtggatga agattttagc ctgaaaaatt
     5041 cgcaattcga cttcctgcat ccgtggtatg acacacccga ttatctgctc ttcagccagc
     5101 atacccttca ccgaacagac gatcgtaccc agatcaacac cggtttgggc tggcgtcatt
     5161 tcacctccag ctggatgtca ggcatcaacc tgttttttga ccacgacctg agccgctatc
     5221 actcccgcgc ggggcttggc gcagaatact ggcgtgatta tctgaagttg agcagcaacg
     5281 cttatattgg cctgaccggc tggcgtagcg caccagaatt ggattatgac ttcgaagccc
     5341 gcccggccaa cggttgggat ttacgcgcgg aaggctggtt acctgcctgg ccacaactgg
     5401 ggggaaaact ggtctatgaa caatactatg gcgatgaggt ggcgctgttt gacaagaacg
     5461 atcgccaaag taatccccat gctattaccg cgggcctcaa ctatacccct ttcccgcttc
     5521 tgactctcag tgcggaacag cgtcagggga agcaaggtga aaatgacaca cgttttgccg
     5581 ttgatctgac ctggcaaccc agcagttcaa tgcagaaaca gcttaacccg gacgaagtgg
     5641 ccggacggcg cagtctggcc ggtagccgtt atgacctgat tgatcgcaac aacaacatcg
     5701 ttctggaata ccgcaagaaa gagctgattc gcctgagtct gctggatccg gtgaaaggga
     5761 agtctggaga aataaaaccg ctggtttcct cgctacagac caaatatgcc ctgaaaggct
     5821 ataacatcga agccgctgcg ctggaagctg ccggtggtaa agtcagcacg tctggaaaag
     5881 atatcacggt cacgctgcca ggttaccgct tcactaacac cccagaaacc gataatacat
     5941 ggtcgataga cgttaccgcc gaggatgtaa aaggtaacct gtcacggcat gaacaaagca
     6001 tggtagttat tcaggctccg acattaagcc agaaagattc tctgttatcc gttaatccgc
     6061 taaccgtggc tgcagataaa aaatcgacga ccacattgac cgttactgcg cacgattccg
     6121 acggaactcc ggtgccgggg ctggcgctgc aaacccgcag tgaaggcgtt caggatatca
     6181 ccctgtctga ctggacagat aacggtgatg gtagttacat acagatgctg accgccggaa
     6241 cgacatcagg ttcagtaaca ctgacgccgc aaattaacgg tgagagtgcg gtaaaagaat
     6301 ccatcgtcgt taatatcgtc cctgttgtct catcccgcga ccattcatca ataacaattg
     6361 ataacgtatc gtattatgcc ggagacgaca tcaaggttag ggtggaactg aaagacgaca
     6421 gcaatcaacc ggttgcatat caaaaagagg aattggtaaa agccgttact gtcgaaaaca
     6481 gcaaacctgg cgccacgatt gtctggcacg aagagcagcc gggggtttat gccgcgaatt
     6541 atccggccca taagcaaggg actgcactaa gggcacaact tagccttcac aactggaatg
     6601 ctccactgca atcgcatatt tataacattg aggcaaacca gaatatggct cgcgtcgcca
     6661 cattatcagc gacaaataat gacgtttacg ccgataaaaa gacatttaat accctcacta
     6721 tcaacgtcac tgatgagagt gataatcccc tgataaatca tcaggtcacc tttaagaatg
     6781 aaaaaggaag cgcggaattt gtcgaaccgc cgcagcaaaa tacggatgga tacggtgttg
     6841 ccacaatcaa catggtaagt caggttgcgg aagaaaatac gattagcgcc acgctgccaa
     6901 atggtttttc acaacggata attgcgaaat tcgttagcga ttcgagtacg ccaaaattca
     6961 aacaactggt tgccgatcca gataccatta ttgctggcaa cagccagggc agtactctga
     7021 ccgccatcgt cacagacttt cataacaacc cgttaaaaga tatgaaagtg aattttgtgg
     7081 cacctggtgg ctcgcaactg gacaacacga ccgccacaac agaccagtcc ggtattgtgc
     7141 gggtgcacct gaccagttca aaagctggta gctattccgt cgatgcctcg cttgaggtgg
     7201 ataaaaatat tcaccagtcg gtcacgatca ccgtggtccc aaacagggaa caatcggtaa
     7261 tgaccttgaa tgccgggtcg ggcagtgcga tcgctaacaa tacaaatatc gttaccctga
     7321 ctgccagtgt gaaagatgtt tatggacacc cgttgccgga tgaggatgtg aaatttacct
     7381 tgccagcctc catgaccggg aacttcacgc taagtagtga aaccgcccgc accgatgcaa
     7441 acggtgatgc cgtggtcaca ttgcgaggca caaaagcggg tgagtttaca gttacggcga
     7501 cgctgaccag aaataatacc gttgtttatc agcaagtcac ttttattggg gatacaaaca
     7561 gtgcgcagct ccagccgctg actgcctcat taaattccat tgttgcgggt aacagtacgg
     7621 ggagtaccct gacggcaacg atcctggacg cttaccaaaa tccgcttaaa gaccagttgg
     7681 tcactttcca gagtaacgat gtcactctaa gcggaacaga agtcaccacc aatacgctgg
     7741 gtcaggcgac ggtaacaatg accagcaata ttgccgggca acataacgtc gtggtgagcc
     7801 ggaaagcgca agcctccgat aataaaacgt ttagtttatc agtgctgccg gatgaaagtt
     7861 cggcgaaggt aataagtata accggagccg aaaaaacgat aacggtgggc gaaaacatca
     7921 cgctacggat actcgtccag gacgcgttta acaatgtaat cgcgggtcaa cgcgtcagat
     7981 taagtgcgca gccaacaact aacattacga taggcgatac ggcttacacc gataataacg
     8041 gttatgcgta cgttaacctt ctcagcaccc aacctggggt ttatcaggtg acggcaacgc
     8101 tggacaataa cagtagtagt aaggttgacg tgaatgtggc aaatggcaaa ctcgagttaa
     8161 catcatcgaa accagaaacc acggtccata atagtgaggg tattacgctg accgcaacgg
     8221 cgagaaatgc gcggggtgaa ttgatgccag ggcaaattat cacctttagc gtaacgcctg
     8281 aaggtgcaac gctaagcaat acaggggaag tccttactga ccagtcaggt caggccaaag
     8341 tgacgctgac cagtgacaaa gtgaatgtct ataccgttac ggccataatg ggcaaagatg
     8401 ttcccgttca gagccaggta acggttgcgg ttaaggcaga tgctaaaacg gcacatgttg
     8461 tgagcgtcgt ggcttctcct gacaccatca ccgccgacgg catcgatagc agcaccatca
     8521 cttcacgagt agaagatgat tacggattcc cggttgaagg tgtcgatgtt agttatacct
     8581 tagacaccaa aggtcgcccg gtagttaata ttccaactac gcgtaccgat caatccgggc
     8641 aagtcacggc gacaataacc agtacattgg cagaaacctt aacagtcaat gtgcaagttc
     8701 ctggtacagc caaccaatcc gcagctatta ccttgattgc cggcacggcg gatgaaagta
     8761 agtcaatttt gaaatccgac gttagcactc tgatggctga ctaccagcat agcgcgaaac
     8821 ttacgctaac attgcaagac aagtacggta acccgatagt gacatctgat catctggaat
     8881 ttgtccagtc aggtcccttc gtgaactttc tcaagttgag cgatattgat tacagccaaa
     8941 gaaattatgg cgagtacacc gtgactgtca ctggcggaaa agagggaaca gcgactctca
     9001 ttcccatgct gaacggggtt catcaggcaa acttaagcgt atcgctgaat ctcatccgct
     9061 cgataaaaga aatgtccggt catgtcactg caaacaacca taccttctcc acggctaaat
     9121 tcccgagcga aggttttgca ggagcgtatt acacactcaa caatgataac tttgaagcgg
     9181 gtaaaaccgt tgatgattat atgttttcaa gttcacagag ttgggtgtcg gtcgatgctt
     9241 caggtaaggt ttctttcgca aatatcggcg atcaaacgtc agtcacaata agcgctgttc
     9301 cccgacaagg gggtacaacc taccagacct taattaagct gaaaggctgg tgggtgaata
     9361 atggaaatca taccaatatc tggctagctg ccaatgcgct ctgtcatgct aaaaatgatg
     9421 gatataatct tccaagcatc acacatttga cgtctggcga aaacaaacgc acgcagggat
     9481 cactgtatgg tgaatggggg aacgttggag cgttttccag taattcgcaa tttacacaag
     9541 gagcttactg gacaagtgaa tctgatgatt acaatcggca ctactatgtg cagatgctaa
     9601 ccggtatgac tggaagcgac gctgattcca gcccccaact gaccgcctgc cgtaaatcac
     9661 tataatttct gcagttgtta ttaaaggata cccttttgcg agggtatcct ttcagactga
     9721 taacaaacgc tacattgccg gatgcgctgc gcttatcagg cctatatagc ccctgcaata
     9781 tattgaattt attgtttttg taccccagtc ggcataaacg accactcaag aaactcaaca
     9841 gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc agaaagggat gcctcgtttc
     9901 gccatttcct gtgctttgac ggttaagaca accggcgatc cgtgtcttgc tcccactttg
     9961 cgtgccgttg cttcatcagc agagagatgc acgtaatgac gatcccctgc aatcagcccc
    10021 tgttttttta tctcgtcaag gaaacggctt gccgtaccgt ggtagagaaa ctgtggcggt
    10081 gttttttcgg caaaggaaat agcgacctgc gaagtcgaat gcccctgaac cgcgcggatg
    10141 cacctaccat cactggaata actaaaacgc tttttatcgc tggttgcgac cacggtatcc
    10201 agcaatgcac gggttaatcg cttgcccgct ttttgggcac aaagtatgag cttatcgata
    10261 tccgcccatc cttcacggtc cagtacgata ccaatcgcct ccggtttgtg tcggaggaca
    10321 aaacttaaaa acttgctcgt atccgccagc tctttttcgt tgtactttgc catcgcgttt
    10381 tcgtccctgg attttctgtt tttatttacc aatgtcattg tcactgggtt tatgaatttt
    10441 ctgcaactca atcctgcgca gaaccttttc cacacagcgt aatttttccg ctatgcaaaa
    10501 aacgcatagc cagaaacata cttgcatttt ccccataccc acatggctga taaggttttt
    10561 cagttccagg aattttcctg gctcaagcaa cacacaaaca caaaaaaaca atggggattg
    10621 ttatgacaca acaaggggat gctgtagccg gggagctggc gacagaaaaa gtgggcatta
    10681 aagggtatct ggcttttttt ctcaccataa tctttttttc cggtgttttt tccggaacag
    10741 acagctggtg gcgcgttttt gatttttccg tgctgaacgg atcgtttggg caactgcctg
    10801 gtgcgaacgg agccaccacc tcgtttcgcg gcgcgggtgg cgcgggggct aaagatggct
    10861 ttctctttgc gctggagctg gcaccgtcag tcattctctc tctggggatt atttcaatta
    10921 ccgatggtct tggcggatta cgcgccgcgc agcagttaat gacacctgtg ctaaaaccgt
    10981 tgttgggcat tccagggatc tgctcgctgg cgctgatcgc taacttgcaa aacacggatg
    11041 ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga aattaccgaa cgcgataaag
    11101 tgattttcgc cgcatatcaa accagcggta gcgccatcat caccaactac ttctcttccg
    11161 gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt acctttagcc gtcatcctgg
    11221 tgtttaaatt tgtcggagcc aacattttgc gcgtctggct taacttcgaa gaacgtcgca
    11281 acccaacgca aggagcgcaa gcatgacaac tcaggtccgc aaaaacgtca tggatatgtt
    11341 tatcgatggc gcgcgccgtg gctttactat cgccacgacc aacctgctgc caaacgtggt
    11401 gatggcgttt gtcatcattc aggcgctgaa aattaccggc ctgctcgact gggtggggca
    11461 tatctgtgaa cccgtaatgg cgctgtgggg attacccggt gaagcggcaa ccgtcctttt
    11521 agccgcatta atgagtatgg gtggcgcagt cggtgttgcc gccagtctgg caaccgctgg
    11581 cgcattaacc ggtcacgatg tcaccgtttt actgcccgcg atgtatttaa tgggcaaccc
    11641 ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg aatgccaaat attacccgca
    11701 tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc tgggttatgc agcttattgt
    11761 ttaacaagga gttaccatga ttgattatac cgcagccggt tttaccctgc tgcagggagc
    11821 gcatttgtat gcgccggaag atcggggaat ttgcgatgtc ctcgtcgcta acggcaaaat
    11881 tatcgccgtt gccagcaata tcccttctga cattgtaccg gactgcacgg ttgtcgatct
    11941 cagcgggcag atcctctgcc caggttttat tgatcaacac gtccatttga ttggcggtgg
    12001 cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg ctaagtcgcc tgacggaagc
    12061 gggcgtcacg tcagtggttg gtctgctggg caccgactct atctctcgcc acccggaatc
    12121 cctgctcgcc aagacccgtg cgctcaatga agaaggcatc agcgcctgga tgctgaccgg
    12181 cgcttatcat gtcccttccc gcaccattac gggttccgtg gaaaaagacg tggcgattat
    12241 cgatcgtgtg attggtgtga aatgcgccat ctctgatcac cgttctgccg caccggacgt
    12301 ttatcacctg gctaatatgg cggcagaatc ccgcgttggc ggtttgctcg gcggtaaacc
    12361 tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg ttacagccta tctatgacct
    12421 gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg acccacgtta accgcaacgt
    12481 accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc ggcaccatcg atatcaccag
    12541 cagcattgac gaaccggtcg cccctgccga aggtattgcc cgcgccgttc aggcgggtat
    12601 tccgctggca cgcgtcaccc tcagctccga cggcaacggt agccagccgt tcttcgacga
    12661 cgaagggaat ttaacccata tcggtgttgc aggttttgaa acgttgctgg aaaccgtgca
    12721 ggtgctggtc aaagactatg atttcagtat cagcgatgcc ctgcgcccgc tcaccagtag
    12781 cgtagccggt ttccttaacc tgagcgggaa aggcgaaatt ctgccaggca atgacgcaga
    12841 cttactggtc atgacgccag aattgcgcat tgagcaggta tacgctcgcg gcaaactgat
    12901 ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa acggcttaaa cggttgtcag
    12961 ccgccctaat ggacgtagaa tgcccgcaag ggcggctgac agagtaaaac gtaatggatg
    13021 actgtggtga ggttttgcat aatattgaaa ccaaatggct ttatgatttt ctgaccctgg
    13081 aaaaatgccg caatttttcc caggcggcag tcagtcgtaa cgtctcgcaa ccggcattca
    13141 gccgccgcat ccgcgcgctg gaacaggcga ttggcgttga attgtttaac cgccaggtga
    13201 cgccgctgca actctcagaa caagggaaaa tcttccattc gcagatccgc catctgttgc
    13261 aacagttaga aagtaacctt gcagagctgc gtggcggcag cgattacgcg caacgtaaaa
    13321 tcaagatagc cgctgcacac tctctttccc tcggtctgtt accgtccatt atcagccaga
    13381 tgccgccgct ctttacctgg gcgattgaag ctattgatgt cgatgaagcg gtcgataaac
    13441 tgcgcgaagg gcaaagtgac tgtatttttt cctttcacga cgaagatttg ctggaagcgc
    13501 cgtttgacca cattcgctta tttgaatctc aattgttccc cgtttgcgcc agtgacgaac
    13561 acggagaagc actttttaac ctcgcgcagc cacactttcc gttactgaat tacagccgca
    13621 actcctacat ggggcgattg attaatcgca ccctgacgcg ccacagtgag ttaagtttca
    13681 gcaccttttt tgtctcttcg atgagcgagc ttttaaagca ggttgccctc gacggctgtg
    13741 ggattgcctg gctgccggaa tacgccatcc aacaagaaat tcgcagcggg cagctcgttg
    13801 tactgaatcg ggacgaactg gtgatcccga ttcaggccta tgcataccga atgaataccc
    13861 gcatgaatcc agttgccgaa cgcttctggc gtgaactgcg cgagctggag attgtgctta
    13921 gctgacattc tccagcgtcg cactgcgcca accgccatta tcccagacca gatcaaacgt
    13981 tagcggttca tcgtcgtggt acagcattat ctcaaggctg atcgccagag tgtggcagta
    14041 cgcttctttc aaattaacct gcaacgccgc aatccgtggc tcatgaaata acagtgcaga
    14101 acgcagctga cggcaatacc agctctggag atgtttgtcg ctcgggttat gcacttgcca
    14161 ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa tgaggagaat taaaaagagt
    14221 gaggagatcg ctgcataccg atttacgcag caatgacgtt ttattgcctg aaattttagg
    14281 taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact cctgacgttt agcaacatat
    14341 gcggcttgcc ctgaacaccg ggcaccactg tattgatgct cattttccat agccattccc
    14401 ttttgttcat tacatccatg tcagtctgag acaggcctga atcctgtcag caaagtataa
    14461 aaccttactt gcctatgaat atctactgac ttttcaggaa aaaagtcatg atttgataaa
    14521 aatcatcaaa ctcaaaaaaa aaccgctagt atattctgaa tattccgaat agttacgtag
    14581 at
//
LOCUS       DAPGRN010000116        14298 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPGRN010000116 DAPGRN010000000
VERSION     DAPGRN010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.118"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(132..884)
                     /gene="yecC"
                     /locus_tag="RHW33_003783"
     CDS             complement(132..884)
                     /gene="yecC"
                     /locus_tag="RHW33_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV1647242.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPRTRQFLEKFLLQ"
     gene            complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="RHW33_003784"
     CDS             complement(881..1549)
                     /gene="tcyL"
                     /locus_tag="RHW33_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV1647243.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRTILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="RHW33_003785"
     CDS             complement(1564..2550)
                     /gene="dcyD"
                     /locus_tag="RHW33_003785"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV1647244.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="RHW33_003786"
     CDS             complement(2655..3455)
                     /gene="tcyJ"
                     /locus_tag="RHW33_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1647245.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="RHW33_003787"
     CDS             complement(3543..4094)
                     /gene="fliZ"
                     /locus_tag="RHW33_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV1647246.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="RHW33_003788"
     CDS             complement(4140..4859)
                     /gene="fliA"
                     /locus_tag="RHW33_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV1647247.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5179..6675)
                     /gene="fliC"
                     /locus_tag="RHW33_003789"
     CDS             complement(5179..6675)
                     /gene="fliC"
                     /locus_tag="RHW33_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H48 family flagellin FliC"
                     /protein_id="HDV1647248.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDL
                     KQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAGTDTGGTNP
                     ASIEGIYTDNGNDYYAKIAGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKA
                     TTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYVADVNE
                     TTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGL
                     TAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLS
                     EAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6941..8347
                     /gene="fliD"
                     /locus_tag="RHW33_003790"
     CDS             6941..8347
                     /gene="fliD"
                     /locus_tag="RHW33_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV1647249.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8372..8782
                     /gene="fliS"
                     /locus_tag="RHW33_003791"
     CDS             8372..8782
                     /gene="fliS"
                     /locus_tag="RHW33_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV1647250.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEDSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8782..9147
                     /gene="fliT"
                     /locus_tag="RHW33_003792"
     CDS             8782..9147
                     /gene="fliT"
                     /locus_tag="RHW33_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV1647251.1"
                     /translation="MNHAPHLYFAWQQLVEKSQIMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9225..10712
                     /gene="amyA"
                     /locus_tag="RHW33_003793"
     CDS             9225..10712
                     /gene="amyA"
                     /locus_tag="RHW33_003793"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV1647252.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESIVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10746..11159)
                     /locus_tag="RHW33_003794"
     CDS             complement(10746..11159)
                     /locus_tag="RHW33_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV1647253.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11346..12551
                     /gene="yedE"
                     /locus_tag="RHW33_003795"
     CDS             11346..12551
                     /gene="yedE"
                     /locus_tag="RHW33_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV1647254.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPVLATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12548..12781
                     /gene="yedF"
                     /locus_tag="RHW33_003796"
     CDS             12548..12781
                     /gene="yedF"
                     /locus_tag="RHW33_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV1647255.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12890..13558
                     /locus_tag="RHW33_003797"
     CDS             12890..13558
                     /locus_tag="RHW33_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV1647256.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRTDGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPDAAREWMRQDIGGKEASEIAASGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            13669..14148
                     /locus_tag="RHW33_003798"
     CDS             13669..14148
                     /locus_tag="RHW33_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1647257.1"
                     /translation="MFTIKTDDLTHPAVQALVAYHISGMLQQSPPESSHALDVQKLRD
                     PTVTFWSVWEGEQLAGMGALKLLDDKHGELKSMRTAPNYLRRGVASLILRHILQVAHD
                     RCLHRLSLETGTQAGFTACHQLYLKHGFADCEPFADYRLDPHSRFLSLTLCENNELP"
BASE COUNT         3492 a         3678 c         3552 g         3576 t
ORIGIN      
        1 tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt
       61 taagcagttt tatcaggctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg
      121 attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctcg
      181 ggatcggcaa ataacgcttt tgcggccccc tgctcgacta tccgcccctg gtccataaag
      241 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gtgtcacaat caccatcgtg
      301 cgcttttcct gcgccagctg acggatggtg ttcaggactt cgcccaccag ctctggatcc
      361 agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca
      421 atcgcaacac gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
      481 gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
      541 cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa
      601 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
      661 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatggt tcccgcttcc
      721 ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg
      781 ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga
      841 ccgtggaatt ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct
      901 ggcgattaag ttgattctca aaatggttct gcaacgtcga taacacagtc gccatgatcc
      961 agtagatgag cgaagccgcc agatacatgg tgaacacctc cagcgtacgc gaggtaatca
     1021 actgcgcctg acggaacagc tctggcacct ggattgtcgc ggccagcgac gtatctttta
     1081 ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
     1141 tcgtgcgacg catggtctgc cacggcgtca taccaatact ggctgccgct tcccactgac
     1201 ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc agcataggcg gcagtgttca
     1261 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
     1321 cgtaatagat cataaacagt tgtgcgatga gcggcgtacc acgaaaaata gagatataaa
     1381 agcgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
     1441 cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
     1501 tcagcaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
     1561 gagctaaacg tggggatgat aggcgaacag cgcaggcgcg ccgccggtat gaataaacag
     1621 aatcggccct tcatctttga agcgtttctg actgataccg tcaatcagac ccgccatcgc
     1681 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
     1741 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccaaagtaa
     1801 aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
     1861 tttcggcaat tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
     1921 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac
     1981 taccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
     2041 cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat atgggcgaaa
     2101 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
     2161 gtcgcacatt tcaatctggg tattgaacag atccagcaac aagcgattgc cgttggttaa
     2221 atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt gcagaccgag
     2281 tttcgccgca actgcggcag tctgccgcac atggttagac tggatcgccc cggcagtaat
     2341 cagcgtatcg gcaccttcgc gcagagcatc tgccgcgaga aattctagct tacgtaattt
     2401 attgccgccc attgccatgg gggtgacgtc atcccgtttg atgaaaattt cccgtcctag
     2461 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccagccg
     2521 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
     2581 tcttattatg cacgctgaaa acgcgtaaat aaaaaagacg ctagtgaaag cgcccttttt
     2641 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagagcttgc
     2701 agagtgccgt ctttttgcat ttccgcaatt gcatcattca ccgctttcag caggtcttca
     2761 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
     2821 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacgatcaac gaggatcgca
     2881 tcgatacgcc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
     2941 tcgacaccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc
     3001 acttttttgc ctttcagatc atcagccgtt ttaatggtgc cttcgttacc ttttttcacc
     3061 agcgcctgaa taccggaaat ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg
     3121 tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc
     3181 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
     3241 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ctccctgaaa actgaacggc
     3301 ggataagttc cttccagccc taccagcagc gtgccgcgct ctttaacttt attaagcaga
     3361 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgctacggc catcacaccc
     3421 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
     3481 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
     3541 atttaatata tatcagaagc aggctggctg gaggatttct gcacaagtcc ggtacacgtt
     3601 tgacgctgat agtgttgata cttccgtaac gcgatgcggt aattgttggt gctggtggtg
     3661 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata
     3721 ttttgctcgt gtagatgatt ccccaaacgg cgcaaacgaa cgacatattc acgcaccgta
     3781 cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
     3841 tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgatttt
     3901 tgttccgttt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
     3961 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgcg
     4021 caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
     4081 tgctgcacca tcattaagaa ctcctggtag tcaaagttaa agtgcggcat ttactgacgt
     4141 tataacttac ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc
     4201 cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa
     4261 tagagggtta ataccagttt ttcgcgctcc ggcagcgttt cgatggcttc catcacccgc
     4321 tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta
     4381 accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
     4441 ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct
     4501 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
     4561 acttcacgcg cgttgcgacg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
     4621 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg
     4681 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
     4741 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
     4801 taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
     4861 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
     4921 atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa attgcggaca
     4981 gaaaaaaccc cgccggtggc gggggagcac gttgctgaca aattgcgctt tatgttgccg
     5041 gatgcggcgt aaacgcctta tccggcctac aaaaatgagc aaattcaata aattgcaatt
     5101 caccttgtag gcctgataag cgcagcgcat caggcaattt ggcgttgccg tcagtctcaa
     5161 ttaatcaggt tacgacgatt aaccctgcag cagagacaga acctgctgcg gtacctggtt
     5221 ggcttttgcc agcacggagt taccggcctg ctgaatgatc tgcgctttcg acatgttgga
     5281 cacttcggtc gcatagtcgg cgtcctgaat acgggactgc gcttcggaca ggttggtagt
     5341 ggtgttgttc aggttggtga ccgcggaatc cagacggttc tgcaccgcac cgagggaaga
     5401 acggaatttg tctacagatg cgatagcatc gtccagcgct ttcagcggat ccgtggtttt
     5461 accatttgca acagcagtca gagcctcacc accagcagtc aaacctgttt gcagattacc
     5521 gccatttaaa tcggcagaat cgtatgtttt accatcaata tcgaccactt ctgttttgcc
     5581 atcatctccg cccagtttga ccgcggttgg agaactggcg gcaccggaag agtcagtata
     5641 ggtaatagtt ttaacagaaa cagcaccagt agtttcattc acatccgcaa cgtaaagatt
     5701 gccatttgta tctttaagcg catatgtatc ggtatcatta cccttggaat cctgcagttt
     5761 tactaagcta acagcaccaa ggttggcagt tgcggaacct gcagtattat caatctgaac
     5821 aggtgtaccg cctgaagtga tagttgtagc tttagtagta tttgcatcag ttacagttgc
     5881 atttgccgtt gctccagtcg ccattgtcac tgtaccatca ttagcaactg ttactgcgta
     5941 atacttccca tcgttatcac caccggcgat tttcgcatag taatcattac cattatcagt
     6001 ataaataccc tcaattgaag ctgggttagt tccgccagta tcagtgcctg cttccgtaga
     6061 aagggtaatt ccagtaagtt taatattgtt tgtggtggtg gcaccaaaag cagttactgg
     6121 agcactagtg gtaactgtat cgttattttt aacactaaaa ccatcaaggc caagagtttt
     6181 agcatcaatc tgcttcagat cgatagtgat agtctggtta tcatttgcgc caacctggat
     6241 tttcatggag ccattttttg ccagcacgtt cacgccgttg aactgggtct gaccagatac
     6301 gcggtcaatt tcatccagac gggatttaat ttcgtcctgg atagaagaca gatcagactc
     6361 agagttagta ccggtagtgg cctgtaccgt cagttcacgc acacgctgta agttgttgtt
     6421 gatttcggac agcgcgcctt cggtggtctg cgcaacggag ataccgtcgt tggcgttacg
     6481 ggccgcctga gtcaggcctt taatgttaga ggtgaaacgg ttagcaatcg cctgacccgc
     6541 tgcgtcatcc ttcgcgctgt taatacgcaa gccagaagac agacgctcga tagaactcga
     6601 cagcgcagac tggttcttgt tgatattatt ttgagtgatc agcgagaggc tgttggtatt
     6661 aatgacttgt gccatgattc gttatcctat attgcaagtc gttgattacg tattgggttt
     6721 ccacccgtcg gctcaatcgc cgtcaaccct gttatcgtct gtcgtaaaac aacctttaga
     6781 atttttttca caaacagcca ttttttgtta gtcgccgaaa tactcttttc tctgcccctt
     6841 attcccgcta ttaaaaaaaa caattaaacg taaactttgc gcaattcagg ccgataaccc
     6901 cggtattcgt tttacgtgtc gaaagataaa aggaaatcgc atggcaagta tttcatcgct
     6961 gggagtcggg tcaggtctgg atttaagttc aatccttgat agcctcaccg ccgcgcaaaa
     7021 agcgacgcta acccctattt caaatcagca atcgtcgttt accgctaaac ttagcgccta
     7081 cggtacgctg aaaagcgcgt tgacgacttt ccagaccgcc aatactgcat tgtctaaagc
     7141 cgatcttttt tccgccacca gcaccaccag cagcaccacc gcgttcagtg ccaccaccgc
     7201 gggtaacgcc atcgccggga aatacaccat cagcgtcacc catctggcgc aggcgcaaac
     7261 cctgaccacg cgcaccacca gagacgatac gaaaacggcg atcgccacca gcgacagtaa
     7321 actcaccatt caacaaggcg gcgacaaaga tccgattacc attgatatca gcgcggctaa
     7381 ctcgtcgtta agcgggatcc gtgatgccat caacaacgca aaagcgggcg taagtgcgag
     7441 catcattaac gtgggtaacg gtgaatatcg tctgtcagtc acatcaaatg acaccggcct
     7501 tgataatgcg atgacactct cggtcagcgg tgatgatgcg ctacaaagtt ttatgggcta
     7561 tgacgccagt gccagcagca acggcatgga ggtctcggtc gccgcccaga atgcgcagct
     7621 gacggtcaac aacgtcgcca ttgagaacag cagcaacacc atcagcgacg cgctggaaaa
     7681 catcaccctg aacctgaacg atgtcaccac gggcaaccag acgctaacca tcactcagga
     7741 cacctccaaa gcgcaaacgg cgattaaaga ctgggtgaat gcctataact cgctaataga
     7801 taccttcagc agcctgacca aatacaccgc cgtagatgcg ggagctgata gccagagttc
     7861 tagcaatggc gcactgctcg gcgactccac gctgcggacg attcagacgc agttgaagtc
     7921 gatgctgagt aataccgtca gttcttccag ctataaaacg ttggcgcaga ttggtatcac
     7981 gaccgatccc agcgatggca aactggaact ggatgccgac aaactcaccg ctgcactgaa
     8041 aaaagatgcc agcggcgtag gtgcattgat tgttggcgat ggtaaaaaaa ccggcatcac
     8101 gaccaccatc ggcagcaacc tgaccagttg gctttcgaca acgggcatta ttaaagccgc
     8161 taccgatggc gttagtaaga ccctgaataa attaactaaa gactacaacg ccgccagcga
     8221 tcgcattgat gcgcaggtcg cgcgctacaa agaacaattt acccaactgg acgttttaat
     8281 gacctcgtta aacagcacaa gcagctactt aacgcagcag ttcgaaaaca acagtaattc
     8341 caagtaatca ttattcatcg ggagacaggt catgtacgcg gcaaaaggca cccaggccta
     8401 tgcacaaatt ggcgtcgaaa gcgccgtaat gagcgccagc cagcagcagc tggtcaccat
     8461 gctatttgat ggagtgctga gcgcactggt tagagcgcgc ctgtttatgc aggacaacaa
     8521 tcagcaaggc aaaggcgtct ctttgtcaaa agcgatcaac attattgaga acggacttcg
     8581 ggtgagtctt gatgaagata gcaaagacga attaacccaa aacttgattg ctctttatag
     8641 ctatatggtc aggcgcttgc tgcaagccaa tttacgcaac gatgtctccg cagtcgaaga
     8701 agtggaagca ttaatgcgta atattgccga tgcctggaaa gagtcgttac tctccccttc
     8761 tttgattcag gacccagtct gatgaaccat gcaccgcatt tatatttcgc ctggcaacaa
     8821 ctcgtcgaaa aaagccagat catgttacgc ctggcaacgg aagaacaatg ggacgaactc
     8881 atcgccagcg aaatggcgta tgttaatgcg gtgcaggaga ttgcacattt gactgaagag
     8941 gttgacccgt ccaccacgat gcaggagcag ctccgcccga tgctgcgcct gattctcgac
     9001 aacgaaagca aggtaaagca gttattacag attcggatgg atgaactggc gaaactggtc
     9061 ggtcagtcat cggtgcaaaa atcggtgtta agtgcctatg gcgatcaggg cggctttgtg
     9121 ctggctccgc aggataacct cttttgaatc tgaatgagtc gatggctcgc gaataatccg
     9181 attacggcta cgcttctaat gttccccttg aatggagtcg aagaatgcgt aatcccacgc
     9241 tgttacaatg ttttcactgg tattacccgg aaggcggtaa gctctggcct gaattggccg
     9301 agcgcgccga cggttttaat gatattggta tcaatatggt ctggttgccg cccgcctata
     9361 aaggcgcatc gggcgggtat tcggtcggct acgactccta tgatttattt gatttaggtg
     9421 agtttgatca gaaaggcagc atccctacta aatatggcga taaagtacaa ctgctggccg
     9481 ccattgacgc cctgaaacgt aatgacattg cggtgctgtt ggacgtggta gtcaaccaca
     9541 aaatgggcgc ggatgaaaaa gaagctattc gcgtgcagcg tgtaaatgct gacgaccgta
     9601 cgcaaattga tgaagaaatc attgagtgcg aaggctggac gcgttacacc ttccccgccc
     9661 gtgccgggca atactcacag tttatctggg atttcaaatg ttttagcggt atcgaccata
     9721 tcgaaaaccc tgatgaagat ggcatattta aaattgttaa cgactacacc ggcgaaggct
     9781 ggaacgatca ggttgatgat gaattaggta atttcgatta tctgatgggc gagaatatcg
     9841 attttcgcaa tcatgccgtg acggaagaga ttaaatactg ggcgcgctgg gtgatggaac
     9901 aaacacaatg cgacggtttt cgtcttgatg cggtcaaaca tattccggcg tggttttata
     9961 aagagtggat cgaacacgta caggaagttg cgccaaagcc gctgtttatt gtggcagagt
    10021 actggtcgca tgaagtcgat aaactgcaaa cgtatattga tcaggtggaa ggcaaaacca
    10081 tgctgttcga tgcgccgctg cagatgaaat tccatgaagc atcgcgcatg ggacgcgact
    10141 acgacatgac gcagattttc acgggtacat tagtggaagc cgatcctttc cacgccgtga
    10201 cgctcgttgc caatcacgac acccaaccgt tgcaagccct cgaagcgccg gtcgaaccgt
    10261 ggtttaaacc gctggcgtat gccttaattt tgttgcggga aaatggcgtt ccttcggtat
    10321 tctatccgga cctctacggt gcgcattacg aagatgtcgg tggtgacggg caaacctatc
    10381 cgatagatat gccaataatc gaacagcttg atgagttaat tctcgcccgt cagcgtttcg
    10441 cccacggtgt acagacgtta tttttcgacc atccgaactg cattgccttt agccgcagtg
    10501 gcaccgacga atatcccggc tgcgtggtgg tcatgtcgaa cggggatgat ggcgaaaaaa
    10561 ccattcatct gggagagaat tacggcaata aaacctggcg tgattttctg gggaaccggc
    10621 aagagagcat agtgaccgac gaaaacggcg aagcaacctt cttttgcaac ggcggcagcg
    10681 tcagcgtgtg ggttatcgaa gaggtgattt aaattcatcc ccggcggcaa gccggggaga
    10741 tttcattacg gcaatggcgt cggtaacggc gctttatcca gcgcagcggc acactctgcc
    10801 gtcgggcgat ccacacgctc catcgtcata ccatcgtact caatggtgtt accgtcacgc
    10861 tcaacctcgt acagctcacg ttttacggta acgttagtga gatcatcaga catcaatgtc
    10921 aacttgcccg gcacagcaat cacccgctgc cactgacggc aatcaagagt atcgccctct
    10981 ttagtgacaa tcaggctgcc tatcgcttcc ggactgacca gcgcacgttg cggccctttg
    11041 gtctgccagt agcccgccag ccagtccggc gcgggggttt tcacgacatt gttatagttt
    11101 tcgacttcgg cacagcccgc cagcaacatg agcgcgcctg caattgcgag ttttttcatc
    11161 attcgtcctg catgagaaga aaagaggatt gtggcattaa agccctgatg tcgccagcct
    11221 ttccaggttc agagagaaat ttgatctaac tattattttt gtgaattatt accccgaaaa
    11281 taaatttgca tggtgtatga ttcgcgactc ttgtttcaaa ccttctgagt tcagaggcta
    11341 cattcatgtc atggcagcaa ttcaaacacg cctggttgat taaattctgg gcacccatcc
    11401 ctgcggtcat cgcggcgggt attctctcca cttactattt tggcatcact ggcacctttt
    11461 gggctgtcac gggtgaattt acccgttggg gcggtcagct tctgcagctg ttcggtgtcc
    11521 atgctgaaga gtggggttat tttaaaatta tccatctgga aggatcgcca ttaacccgca
    11581 tcgacgggat gatgatcctc ggtatgtttg gcggctgctt tgccgcagcg ctgtgggcca
    11641 acaatgtcaa actacgcatg ccgcgtagcc gtatccgcat tatgcaggcc atcattggcg
    11701 gcattatcgc cggttttggc gcgcgtctgg caatgggctg taacctggcg gcattcttta
    11761 ccggtattcc tcagttctcg ctgcatgcct ggttctttgc catcgccact gccattggtt
    11821 catggtttgg cgcgcgcttt accctgctgc ccatcttccg tattcccgtg aaaatgcaga
    11881 aagtttctgc cgcctcaccg ctgacgcaaa aaccggatca ggcgcggcgt cgttttcgtc
    11941 tcgggatgct ggtctttttc ggcatgctgg gctgggcgct gctcacggcg atgaaccaac
    12001 caaaactggg gctggcaatg ctgtttggcg tcggctttgg tttactgatt gaacgtgcgc
    12061 aaatctgctt tacttcggcg ttccgcgata tgtggatcac cggacgtact catatggcga
    12121 aagcaatcat tatcggtatg gcggtgagtg ccatcgggat cttcagttac gtacagttag
    12181 gtgttgaacc caaaatcatg tgggcgggac caaacgcggt aattggtggt ttactgtttg
    12241 gttttggcat cgtgctggct ggcggctgcg aaaccggctg gatgtaccgc gcggtagaag
    12301 gccaggtgca ctactggtgg gtaggtctgg gcaacgtgat cggctcaacg attctggcgt
    12361 attactggga tgatttcgct ccggtgctgg ccaccgactg ggacaaaatc aacctgctga
    12421 aaacctttgg tccgatgggt ggcctgctgg tgacatattt gctgctgttt gctgcgctga
    12481 tgttgattat cggctgggaa aaacgcttct tccgccgtgc ggcaccgcag actgctaagg
    12541 agatcgcatg aaaaatatcg ttcctgatta ccgccttgat atggtgggtg aaccctgccc
    12601 ttatccggca gtcgccaccc ttgaggcgat gccgcagtta aaaaaaggtg agatcctgga
    12661 agtggtgagc gactgtccgc agtcgatcaa taatattcca ctggatgcgc gtaatcacgg
    12721 ctatacggtg ctggatattc agcaagacgg gccgaccatt cgttatttaa ttcagaagta
    12781 atccctcatt cccgctgtgg cccacagcgg gaatatcgtt gcctctgtac cacatagatt
    12841 ttacccgccc cctctcctat tcttttcagt gtgctctcag gaggtttcta tgtgtggacg
    12901 ctttgcccaa tcccaaacgc gtgaagatta ccttgcgctt ctcgcggaag atattgaacg
    12961 cgatattccc tatgatcccg aacccattgg cagatacaac gtcgcgccgg gaaccaaagt
    13021 ccttctgctc agtgaacgtg acgaacacct tcatctggat ccggttttct ggggatatgc
    13081 tcccggatgg tgggataaac cgccactgat taacgcccgc gtagaaactg ccgcaacgag
    13141 tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc tgttttgccg atggctggtt
    13201 tgagtggaaa aaagaaggcg acaaaaagca gccttatttt atctatcgta ctgatggaca
    13261 acctgttttt atggccgcga taggcagcac accgttcgag cgcggtgacg aagccgaagg
    13321 atttttgatt gtcactgcgg cggcagatca aggtctggtg gatattcatg accgccgccc
    13381 actggtactg tcgccagatg ctgcgcggga atggatgcgg caagatattg gcggtaaaga
    13441 agcctcagaa atcgcggcca gtggttgtgt gccagcaaac cagttcacct ggcacccggt
    13501 atcgcgcgcg gtgggtaatg ttaaaaacca gggtgcggag ttaattcaac ctgtttgata
    13561 agcctggaga ttattgttca acaatactgc gtcataagta atctctatta gacaaagatt
    13621 tcattacctg ttggcatatt gcaaaaataa caccaatacg gaatcgtcat gttcacgatt
    13681 aaaacagatg atctcaccca tccagcagtg caagcattag tggcttacca tatttccggc
    13741 atgctgcagc agtctccccc tgaaagcagt catgctttag atgtgcaaaa attacgtgac
    13801 ccgacagtga cattctggtc agtatgggaa ggcgaacaac tcgcaggaat gggtgcactg
    13861 aagttgctgg atgataaaca tggcgaactg aaatcaatgc gtaccgcgcc aaattattta
    13921 cgtcggggtg tcgccagtct gattttacgc cacattttgc aggtcgccca tgacagatgc
    13981 cttcatcgcc tgagcttaga aacggggaca caggctggat ttacggcctg ccatcaactt
    14041 tatttgaagc atggtttcgc tgattgcgaa ccgtttgctg attatcgact tgatccacac
    14101 agtcgatttt tatcattgac gctatgcgaa aataatgaat tgccatgagc cagacgcagc
    14161 acattcttgc attcgacgtg ctgcgccttt atttatcacc agcaggaaac gccttctcca
    14221 tagacgcccc ttccacatgc gccacaagaa acctctattc cagtgacaca attgcgccta
    14281 attaattaca tataatat
//
LOCUS       DAPGRN010000117        14274 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPGRN010000117 DAPGRN010000000
VERSION     DAPGRN010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.119"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            96..1034
                     /gene="tdcA"
                     /locus_tag="RHW33_003799"
     CDS             96..1034
                     /gene="tdcA"
                     /locus_tag="RHW33_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV1647258.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELVKEYSSYNGCRRRQLIEVG"
     gene            1133..2122
                     /gene="tdcB"
                     /locus_tag="RHW33_003800"
     CDS             1133..2122
                     /gene="tdcB"
                     /locus_tag="RHW33_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV1647259.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVARPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            2144..3475
                     /gene="tdcC"
                     /locus_tag="RHW33_003801"
     CDS             2144..3475
                     /gene="tdcC"
                     /locus_tag="RHW33_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV1647260.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            3501..4709
                     /gene="tdcD"
                     /locus_tag="RHW33_003802"
     CDS             3501..4709
                     /gene="tdcD"
                     /locus_tag="RHW33_003802"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV1647261.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            4743..7037
                     /gene="tdcE"
                     /locus_tag="RHW33_003803"
     CDS             4743..7037
                     /gene="tdcE"
                     /locus_tag="RHW33_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV1647262.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMMRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            7051..7440
                     /locus_tag="RHW33_003804"
     CDS             7051..7440
                     /locus_tag="RHW33_003804"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV1647263.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            7512..8876
                     /gene="tdcG"
                     /locus_tag="RHW33_003805"
     CDS             7512..8876
                     /gene="tdcG"
                     /locus_tag="RHW33_003805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV1647264.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKAQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            9150..10481
                     /locus_tag="RHW33_003806"
     CDS             9150..10481
                     /locus_tag="RHW33_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV1647265.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            10509..11819
                     /locus_tag="RHW33_003807"
     CDS             10509..11819
                     /locus_tag="RHW33_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV1647266.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHDG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11953..12117)
                     /locus_tag="RHW33_003808"
     CDS             complement(11953..12117)
                     /locus_tag="RHW33_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647267.1"
                     /translation="MSKKSAKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(12140..12841)
                     /locus_tag="RHW33_003809"
     CDS             complement(12140..12841)
                     /locus_tag="RHW33_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV1647268.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12946..13842
                     /gene="yhaJ"
                     /locus_tag="RHW33_003810"
     CDS             12946..13842
                     /gene="yhaJ"
                     /locus_tag="RHW33_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV1647269.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            complement(13893..14249)
                     /locus_tag="RHW33_003811"
     CDS             complement(13893..14249)
                     /locus_tag="RHW33_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV1647270.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT         3416 a         3339 c         3839 g         3680 t
ORIGIN      
        1 gtattgaaag ttaatttgtg agtggtcgca catatcctgt tcatttcatt ttgatacact
       61 tcatgccgtc aatgaggtaa ttaacgtagg tcgttatgag cactattctt cttccgaaaa
      121 cgcagcacct ggtagtcttt caggaagtca ttagaagtgg ttctatcggc tcggctgcaa
      181 aagaattagg gttaactcaa ccggccgtca gtaaaatcat taacgatatt gaagattatt
      241 ttggtgtgga attagtggtg cggaaaaata ccggtgtaac attaacacct gccggtcaat
      301 tgttactctc tcgttccgaa tccattaccc gtgaaatgaa aaatatggtt aatgagataa
      361 gcggtatgtc ttctgaggcg gtggtggaag tctcatttgg ttttccttca ttgattggtt
      421 ttacttttat gtcagggatg atcaataagt tcaaagaggt gttcccaaaa gcgcaggttt
      481 ctatgtatga agcgcaactg tcttcgttct taccggcaat ccgcgacggt cgactggatt
      541 ttgcgattgg tacgttaagt gcagaaatga agcttcagga tttacatgtt gagccgctgt
      601 ttgagtccga gtttgtactg gtagccagta agtcccgaac atgcaccggc accaccacgc
      661 tggagtcgtt gaagaacgaa cagtgggtgt tgccacaaac caatatgggg tactacagcg
      721 aactgcttac tacgttacaa agaaatggca tcagtattga aaacatcgtt aaaaccgact
      781 cagtcgtgac aatttataat cttgttctca atgctgattt cttaactgta attccttgtg
      841 atatgacgtc accttttggt tctaatcaat ttattactat tccggttgaa gaaacattac
      901 ctgtggcaca atatgccgcg gtatggtcga aaaattatcg tattaaaaaa gcagcatcgg
      961 ttttggtgga attagtcaaa gagtattcat cttataatgg gtgtagacga aggcaattaa
     1021 tagaagttgg ttagttattt gtttttattt aaacataaat aatccacctg tctgtcttgc
     1081 aggtgtcggt tacggttacc tacatattta attcaggcga agaggtttta taatgcatat
     1141 tacatacgat ctgccggttg ctattgatga cattattgaa gcgaaacaac gactggctgg
     1201 gcgaatttat aaaacaggca tgcctcgctc caactatttt agtgaacgtt gcaaaggtga
     1261 aatattcctg aagtttgaaa atatgcagcg tacgggttca tttaaaattc gtggcgcatt
     1321 taataaatta agttcactga ccgatgcgga aaaacgcaaa ggcgtggtgg cctgttctgc
     1381 gggcaaccat gcgcaagggg tttcgctctc ctgcgcgatg ctgggtatcg acggtaaagt
     1441 ggtgatgcca aaaggcgcgc cgaaatccaa agtagcggca acgtgcgact actccgcaga
     1501 agtcgttctg catggtgata acttcaacga cactatcgct aaagtgagcg aaattgtcga
     1561 aatggaaggc cgtattttta tcccacctta cgatgatccg aaagtgattg ctggtcaggg
     1621 aacgattggc ctggaaatta tggaagatct ctatgatgtc gataacgtga ttgtgccaat
     1681 tggtggtggc ggtttaattg ctggtattgc ggtggcaatt aaatctatta acccgaccat
     1741 tcgtgttatt ggcgtacagt cagaaaacgt tcacggcatg gcggcttctt tccactccgg
     1801 agaaataacc acgcaccgaa ctaccggcac cctggcggat ggttgtgatg tcgcccgccc
     1861 gggtaattta acttacgaaa tcgttcgtga attagtcgat gacatcgtgc tggtcagcga
     1921 agacgaaatc agaaacagta tgattgcctt aattcagcgc aataaagtcg tcaccgaagg
     1981 cgcaggcgct ctggcatgtg ctgcattatt aagcggtaaa ttagaccaat atattcaaaa
     2041 cagaaaaacc gtcagtatta tttccggcgg caatatcgat ctttctcgcg tctctcaaat
     2101 caccggtttc gttgacgctt aattaattcg ttgaggatag gatatgagta cttcagatag
     2161 cattgtatcc agccagacaa aacaatcgtc ctggcgtaaa tcagatacca catggacgtt
     2221 aggcttgttt ggtacggcaa tcggcgccgg ggtgctgttc ttccctatcc gcgcaggttt
     2281 tggcggactg atcccgattc ttctgatgtt ggtattggca taccccatcg cattttattg
     2341 ccaccgggcg ctggcgcgtc tgtgtctttc tggctctaac ccttccggca acattacgga
     2401 aacggtggaa gagcattttg gtaaaactgg cggcgtggtt atcacgttcc tgtacttctt
     2461 cgcgatttgc ccactgctgt ggatttatgg cgttactatt accaatacct ttatgacgtt
     2521 ctgggaaaac cagctcggct ttgcaccgct gaatcgcggc tttgtggcgc tgttcctgtt
     2581 gctgctgatg gctttcgtca tctggtttgg taaggatctg atggttaaag tgatgagcta
     2641 cctggtatgg ccgtttatcg ccagcctggt gctgatttct ttgtcgctga tcccttactg
     2701 gaactctgca gttatcgacc aggttgacct cggttcgctg tcgttaaccg gtcatgacgg
     2761 tatcctgatc actgtctggc tggggatttc catcatggtt ttctccttta acttctcgcc
     2821 aatcgtctct tccttcgtgg tttctaaacg tgaagagtat gagaaagact tcggtcgcga
     2881 cttcaccgaa cgtaaatgtt cccaaatcat ttctcgtgcc agcatgctga tggttgcagt
     2941 ggtgatgttc tttgccttta gctgcctgtt tactctgtct ccggccaaca tggcggaagc
     3001 caaagcgcag aatattccag tgctttctta tctggctaac cactttgcgt ccatgaccgg
     3061 taccaaaaca acgttcgcga ttacactgga atatgcggct tccatcatcg cactcgtggc
     3121 tatcttcaaa tctttcttcg gtcactatct ggggacgctg gaaggcttga atggtctgat
     3181 tctgaagttc ggttataaag gtgacaaaac caaagtgtcg ctgggtaaac tgaatactct
     3241 cagcatgatc ttcatcatgg gctccacctg ggttgttgcc tacgccaacc cgaacatcct
     3301 cgacctgatt gaagccatgg gcgcaccgat tatcgcatcc ctgctgtgcc tgttgccgat
     3361 gtatgccatc cgtaaagcgc cgtctctggc gaaataccgt ggtcgtctgg ataacgtgtt
     3421 tgttaccgtg attggtctgc tgaccatcct gaacatcgta tacaaactgt tttaatccgt
     3481 aactcaggat gagaaaagag atgaatgaat tcccggttgt tttggttatt aactgtggtt
     3541 cgtcttcgat taagttttcc gtactcgatg ccagcgactg tgaagtatta atgtcaggta
     3601 ttgccgacgg tattaactcg gaaaatgcat tcttatccgt aaatggggga gagccagcac
     3661 cgctggctca ccacagctac gaaggtgcat tgaaggcaat tgcatttgaa ctggaaaaac
     3721 ggaatttaaa tgacagtgtg gccttaattg gccaccgcat cgctcacggc ggcagtattt
     3781 ttaccgagtc cgccattatt accgatgaag tcattgataa tatccgtcgc gtttctccac
     3841 tggcacccct gcataattac gccaatttaa gtggtattga atcggcgcag caattatttc
     3901 cgggcgtaac tcaggtggcg gtatttgata ccagtttcca ccagacgatg gctccggaag
     3961 cttatttata cggcctgccg tggaaatatt atgaagagtt aggtgtacgc cgttatggtt
     4021 tccacggcac gtcgcaccgc tatgtttccc agcgcgcaca ttcgctgctg aatctggcgg
     4081 aagatgactc cggcctggtt gtggcgcatc ttggcaatgg cgcgtcaatc tgcgcagttc
     4141 gcaacggtca gagtgttgac acctcaatgg gaatgacgcc gctggaaggc ttgatgatgg
     4201 gtacccgcag tggcgatgtc gactttggtg cgatgtcctg ggtcgccagc caaaccaacc
     4261 agagcctggg tgacctggaa cgcgtagtga ataaagagtc gggattatta ggtatttccg
     4321 gtctttcttc ggatttacgt gttctggaaa aagcctggca tgaaggtcac gaacgcgcgc
     4381 aactggcaat taaaaccttt gttcaccgaa ttgcccgtca tattgccgga cacgcagctt
     4441 cattacgtcg cctggatgga attatattca ccggcggaat aggagagaat tcaagtttaa
     4501 ttcgtcgtct ggtcatggaa catttggctg tattaggcgt agagattgat acagaaatga
     4561 ataatcgatc taactctttt ggcgagcgaa ttgtttccag tgaaaatgcg cgtgtcattt
     4621 gcgccgttat tccgactaac gaagaaaaaa tgattgcttt ggatgccatt catttaggca
     4681 aagttaacgc gcccgcagaa tttgcataat ttagttgaag tattgtagag agattatttt
     4741 tcatgaaggt agatattgat accagcgata agctgtacgc cgacgcatgg cttggcttta
     4801 aaggtacgga ctggaaaagc gaaattaatg tccgcgattt tattcaacat aactatacac
     4861 cgtatgaagg cgatgaatct tttctcgccg aagcgacgcc tgccaccacg gaattgtggg
     4921 aaaaagtaat ggaaggcatc cgtatcgaaa acgcaaccca cgcgccggtt gatttcgata
     4981 ccaatattgc caccacaatt accgctcatg atgcgggata tattaaccag ccgctggaaa
     5041 aaattgttgg cctgcaaacg gatgcgccgt tgaaacgtgc gctacacccg ttcggtggca
     5101 ttaatatgat taaaagttca ttccacgcct atggccgtga aatggacagt gaatttgaat
     5161 atctgtttac cgatctgcgt aaaacccata accagggcgt atttgatgtt tactcaccgg
     5221 atatgctgcg ctgccgtaaa tctggcgtac tgaccggttt accagatggt tatggacgtg
     5281 ggcgcattat aggtgactat cgccgcgtag cgctgtatgg cattcgttat ctggtgcgtg
     5341 aacgcgaact gcaatttgcc gatctccagt ctcgtctgga aaaaggcgag gatctggaag
     5401 ccaccatccg tctgcgtgag gagctggcag agcatcgtca tgcgctgttg cagattcagg
     5461 aaatggcggc gaaatatggc tttgatatct ctcgcccggc gcagaatgca caggaagcgg
     5521 tgcagtggct ctacttcgct tatctggcgg cagtgaaatc acagaacggc ggcgcgatgt
     5581 cgctgggtcg cacggcatcg ttcctggata tctacattga gcgcgacttt aaagctggcg
     5641 tcctcaatga gcagcaggca caggaactga tcgatcactt catcatgaag atccgtatga
     5701 tgcgcttctt gcgtacgccg gaatttgatt cgctgttctc cggcgaccca atctgggcga
     5761 cggaagtgat cggcgggatg gggctggacg gtcgtacgct ggtgaccaaa aactccttcc
     5821 gctatttgca caccctgcac actatggggc cggcaccgga acctaacctg accattcttt
     5881 ggtcggaaga attaccgatt gccttcaaaa aatatgccgc gcaggtgtcg atcgtcacct
     5941 cttccttgca gtatgaaaac gacgatctga tgcgtactga cttcaacagc gacgattacg
     6001 cgattgcctg ctgcgtcagc ccaatggtga ttggtaagca aatgcagttc tttggtgcac
     6061 gcgctaacct ggcgaaaacg ctgctgtacg caattaacgg cggggtggac gagaagctga
     6121 agattcaggt cgggccgaaa acagcaccgt tgatggacga tgtgctggat tacgacaaag
     6181 tgatggacag cctcgatcac ttcatggact ggctggcggt gcagtacatc agcgcgctga
     6241 atatcattca ctacatgcac gacaagtaca gctacgaagc ttcgctgatg gcgctgcacg
     6301 atcgtgatgt ctatcgcacc atggcgtgcg gcatcgcggg cctgtcggtg gcaacggact
     6361 ccctgtctgc gatcaaatat gcccgcgtga aaccaatccg tgacgaaaac ggcctggcgg
     6421 tggactttga aatcgacggt gaatatccgc agtacggcaa caacgacgag cgcgtagaca
     6481 gcattgcctg cgacctggtt gaacgtttta tgaagaaaat taaagcgctg ccaacctatc
     6541 gcaacgccgt ccctacccag tcgattctga ctatcacttc taacgtggtg tacggccaga
     6601 aaaccggtaa tacgccggac ggtcgtcgcg ccggaacacc gttcgcgccg ggcgctaacc
     6661 cgatgcatgg tcgtgaccgc aaaggtgccg tggcctcatt gacgtcggtg gcgaaactgc
     6721 cgttcaccta cgctaaagac gggatctcgt acaccttctc gattgtgcct gcggcgctgg
     6781 gcaaagaaga tccagtacgt aaaaccaacc ttgtcggcct gctggatgga tatttccacc
     6841 acgaagcgga tgtcgaaggc ggtcaacacc tcaacgtcaa cgtaatgaat cgggaaatgc
     6901 tgctggatgc catcgagcac ccggaaaaat atcctaacct gacaatccgt gtctctggct
     6961 acgccgtgcg cttcaacgca ctgacccgtg aacagcagca ggatgttatt tcacgtacct
     7021 ttacccaggc gctctgacgc cggaggatgt atgaaaaaga ttatcgaaac gcaacgtgcc
     7081 ccaggcgcaa tcggccctta tgttcaaggc gttgatttag gcagcatggt cttcacctcc
     7141 ggtcaaattc cggtttgccc acagaccggt gagatcccgg ctgatgtgca agatcaggcg
     7201 cgtttaagcc tcgaaaacgt caaagcgatc gtggttgctg ccgggctgag cgtgggcgat
     7261 atcatcaaga tgaccgtgtt catcaccgat ctgaatgatt ttgccaccat caacgaagtc
     7321 tataagcagt tcttcgatga gcatcaggcg acctacccga cccggagctg tgtacaggtc
     7381 gcgcgtttgc cgaaagatgt gaagctggaa attgaagcta tcgcagtacg tagtgcgtaa
     7441 agcctcgtga gcgggacggt cgtaaggtcg ttccgctcca cttcactgaa cggcaatccg
     7501 agggtgtgga tatgattagt gcattcgata ttttcaaaat tgggattggt ccctccagtt
     7561 cgcataccgt ggggccaatg aatgccggaa aaagttttat tgatcggctg gaaagtagcg
     7621 gcttattaac cgcgacgagc catattgtgg tcgatctgta cgggtcgttg tcactgacgg
     7681 gcaaaggcca tgccacggat gtcgccatca tcatgggact ggcaggaaac agtccgcagg
     7741 atgttgtcat tgatgagatc cctgcattta tagagttagt aacgcgcagc gggcggctgc
     7801 cagtggcatc tggtgcgcat attgttgatt ttcctgtagc aaagaacatt atcttccatc
     7861 ccgaaatgtt gcctcgccat gagaacggaa tgcggatcac tgcctggaag gcacaggaag
     7921 agctattaag taaaacctat tactctgtcg gcggcgggtt tattgtcgaa gaagaacact
     7981 tcggcctgtc gcacgatgtc gaaacgtccg taccttacga tttccactca gcaggtgaac
     8041 tgctgaaaat gtgtgattac aacggcctgt ctatttctgg cctgatgatg cacaacgagc
     8101 tagcgctgcg cagtaaagcg gaaattgacg ccggttttgc ccgtatctgg caagtgatgc
     8161 atgacggtat tgaacgcggg atgaacactg aaggcgtgct gcctggtccg ctcaatgtgc
     8221 cgcgccgtgc cgtagcgctg cgtcgtcagc tggtttccag cgataacatc tctaacgatc
     8281 cgatgaatgt catcgactgg atcaacatgt acgcgctggc ggttagtgaa gaaaacgcag
     8341 ctggcgggcg cgtggtaacg gcaccgacta acggtgcgtg cggcattatt ccggcagtgc
     8401 tggcttatta cgataagttc cgtcgtccgg taaacgagcg gtcaattgcc cgctattttc
     8461 ttgcagccgg ggctattggc gcgctgtata aaatgaacgc ctccatctct ggcgcggagg
     8521 tcggctgtca gggggagatt ggcgtggcct gttcaatggc ggcggcaggg ttaactgaac
     8581 tactgggcgg cagtccggcg caggtatgca atgcggcgga aatcgcgatg gagcataacc
     8641 ttggactgac ctgcgatccg gttgccggac aggtacaaat cccgtgcatt gaacgtaatg
     8701 ccattaatgc cgtgaaagca gtaaacgccg cgcggatggc gatgcgccgc acctcggcac
     8761 cgcgtgtttc actcgataaa gtgatcgaga cgatgtatga aaccggcaaa gatatgaacg
     8821 ataaataccg cgaaacatca cgcggaggac tggccattaa agtggtctgc ggctgacatt
     8881 gctgtcagct ttcatccttg ggtgcacaaa tgtgcacctt tttttgtgat ctgccgcaaa
     8941 tagagaatga atattctctt gcgattaatt tagtagaaaa ttcttatcca tctccgttat
     9001 ttcaaagtct tgattgtgaa gattttctct gacctcgcgc gtatcatttc tttattctaa
     9061 taactcgcct gcaaaaacgt agaccgcata tcgccttgat gcggctcgga aaaaaataat
     9121 aagccctaca aaatattgag cgagaaatta tggaaattgc atcgaataaa ggcgtcattg
     9181 cagacgcttc gaccccggcg ggtcgtgctg gaatgagtga gagcgagtgg cgagaagcga
     9241 tcaaattcga cagtactgac accggctggg tgattatgag tatcgggatg gcgattggcg
     9301 cggggattgt ttttctcccg gtgcaggtcg gtttgatggg attgtgggta tttttgctct
     9361 catcggtgat tggttacccg gcaatgtatc tgtttcagcg gttgtttatt aatacgctgg
     9421 cagaatcacc agaatgtaaa gattacccga gtgtcattag cggttattta ggtaaaaact
     9481 ggggcatcct gttaggtgcg ctctatttcg taatgctggt gatttggatg ttcgtctatt
     9541 ccaccgccat caccaacgat agtgcttcct acctgcatac cttcggcgtg acggaagggt
     9601 tgctgtcaga cagtcccttt tatggtctgg tactgatttg cattctggtg gcgatctctt
     9661 cacgcggcga gaaattgtta ttcaaaattt cgactggcat ggtgctgacc aagctgctgg
     9721 tggtcgcggc gctgggcgtg tcgatggtgg gaatgtggca tctgtataac gtcggttcgc
     9781 taccgccgct ggggctgctg gtgaaaaacg ccattattac gctgccgttt accctgacgt
     9841 cgattctgtt tatccagacg ttaagcccga tggtgatctc ttatcgctcg cgggaaaaat
     9901 caattgaagt ggcgcggcat aaagcattgc gggcaatgaa tatcgcgttt ggcattttgt
     9961 ttgtcaccgt ctttttctac gccgtgtcgt tcacgctggc gatgggacat gacgaagcgg
    10021 taaaagccta cgagcagaat atttccgcgc tggcgattgc cgcacagttt attagcggtg
    10081 acggcgcagc gtgggtgaaa gtggtcagcg tcattctcaa tatctttgct gtcatgactg
    10141 cattctttgg cgtctattta ggttttcgcg aagcaacgca agggatcgta atgaacatcc
    10201 tgcgtcgcaa gatgcctgcc gagaagatta acgaaaatct tgttcagcgc ggcatcatga
    10261 ttttcgccat tttgctggcc tggagcgcca tcgtactgaa cgcaccggtg ttgagcttca
    10321 cctctatctg tagcccgatt ttcggcatgg tagggtgcct gatcccggcg tggctggttt
    10381 acaaagtacc ggcattgcac aaatacaaag ggatgtctct gtacctgatt atcgtcactg
    10441 gtttgttgct ttgtgtttct ccgttcctgg cattttcttg atttacctga aattttaagg
    10501 tttttaatat gtttgattcg actttaaatc cgttatggca gcgttacatc ctcgccgttc
    10561 aggaggaagt aaaaccggcg ctgggatgta ctgaaccgat ttcactggcg ctggcggcgg
    10621 cggttgctgc ggcagaactg gaaggtccgg ttgaacgtgt agaagcctgg gtttcgccaa
    10681 atctgatgaa gaacggtctg ggcgtcaccg ttcccggcac gggaatggtg gggctgccga
    10741 ttgcggcggc gctgggggcg ttaggtggaa atgccaacgc cgggctggaa gtgctgaaag
    10801 acgcaactgc gcaggcaatt gccgatgcca aagcactgct ggcggcgggg aaagtctccg
    10861 ttaagatcca ggaaccttgc aatgaaatcc tcttctcacg cgccaaagtc tggaacggtg
    10921 agaagtgggc gtgtgtcact atcgtcggcg ggcataccaa cattgtgcat attgagacgc
    10981 acgatggtgt ggtgtttacc cagcaggcgt gtgtggcaga gggcgagcaa gagtctccgc
    11041 tgacggtgct ttccagaacg acgctggctg agatcctgaa gttcgtcaat gaagtcccgt
    11101 ttgcggcgat ccgctttatt ctcgattctg cgaagctaaa ttgtgcgtta tcgcaggaag
    11161 gtttgagcgg taagtggggg ctgcatattg gcgcgacgct ggaaaaacag tgcgcgcgcg
    11221 gtttgctggc gaaagatctc tcttcatcca ttgtgattcg taccagcgcg gcatccgatg
    11281 cgcgtatggg cggcgctacg cttccggcta tgagtaactc cggctcgggt aaccagggga
    11341 tcaccgcaac aatgcctgtg gtggtggtag cagaacactt cggagcggat gatgaacggc
    11401 tggcgcgtgc gctgatgctt tctcatttga gcgcaattta catccataac cagttaccgc
    11461 gtttgtctgc gctgtgtgcc gcaacgaccg cagcaatggg ggccgccgcc gggatggcat
    11521 ggctggtgga tgggcgttat gaaaccatct cgatggcgat cagcagtatg atcggcgatg
    11581 tcagcggcat gatttgcgat ggtgcgtcga acagctgcgc gatgaaggtt tcgaccagtg
    11641 cttcggctgc gtggaaagcg gtgttaatgg cgctggatga taccgccgtg accggcaatg
    11701 aagggattgt ggcgcatgat gttgagcagt cgattgccaa cctgtgtgca ttagcaagcc
    11761 attcgatgca gcaaacggat cggcagatta tcgagattat ggcgagcaag gccagataaa
    11821 cgccaggttt tgggtatcgt gccggatgcg atgctggcgc atcttatccg gcctacgggt
    11881 tgtttaattc gttaatgttg ttggattttg taggccggat aaggcgtttt cgccgcatcc
    11941 ggcaacctgt ctttacgcgg tagcttcatc ctcggctaaa cgcgtttcag catccgcgac
    12001 gatggcttcc agctcgctca acatctcttc atagccaaaa tttttggcgg tgcgagcagg
    12061 ttctttcgcc accacgggct tcgctggctg gcgctttttg gccgattttt tactcatcat
    12121 catctccctg taaaaacaac tagacaggca aatctatcag caaagcgcgc agtggggaat
    12181 cggcaaccag cgtaatgtta gcctcgtcac gaataaacgc cccatcaccg caggtcagcg
    12241 ccgctttctc ttcatgatgc gtaagcgcat gaaatttccc gtgaatcgat tgcaaatacg
    12301 cgcgtggccc atgcaactgg aaattcgcac tttcgccttt gtcgagcacg atatggtgca
    12361 gccacacttg ctggcgtaat tgcaggcttc ccatcgcacc ctctggcgag gcgattaatt
    12421 gctgcttgcc catattaagc gccagttttt gaatcagcgg attctctcgc tgcgggcagg
    12481 cgtccagcca aagctgcatt cgtgttaacg gtttgtcttt gctgagattg tgttcgctgt
    12541 agctgacacc cggctgggta gagagcagca acgcctcacc ggcgctggcc tgaacatgat
    12601 tgccttcgct gtcgcgatac tctgcttctc catccagaat cacatttaaa atatcgactt
    12661 tgggataggt tcgcggctga aaggcggcac ctggggccag cacttcctgg ttaagcacac
    12721 gcagggaggc atagcctaac aatttcgggt cgaagtagtg tccaaaggaa aaagtatacc
    12781 gggcctgcaa ccatccgtag tctgcttgtc cacactgcct ggcagttcgg gtagtaatca
    12841 taattcttga cctctcttta cttcctttta tggtaagggg ctggacgcta cattgttagc
    12901 cagatattct gcccggtatg ttcaaatttc ctgaatgaga acgaaatggc caaagaaagg
    12961 gcattaacgc tggaagcact acgggttatg gatgcgatcg atcgccgggg cagttttgcg
    13021 gcggcagcgg atgagctggg acgcgtgcct tccgcactta gctacaccat gcaaaaactg
    13081 gaagaagagc tggatgtggt gctgtttgac cgctcgggcc atcgcaccaa attcaccaat
    13141 gtcgggagga tgttgttgga gcgagggcgc gttttgctgg aagccgcaga taaactgact
    13201 actgatgcgg aagctctcgc gcgcggttgg gaaacgcatc tcaccattgt gaccgaagcg
    13261 ctggtaccga cacctgcctt tttcccgtta atcgacaaac tggcggcaaa agccaatacc
    13321 caactggcaa tcatcacaga agtgctggcg ggggcgtggg aacggctgga gcaggggcgg
    13381 gcggatattg ttatcgcgcc ggatatgcat tttcgttcct cgtcggagat caactcgcgc
    13441 aagctctata cgttaatgaa cgtctacgtt gccgcgcctg atcacccgat tcatcaggag
    13501 ccggaaccgt tatctgaagt gacgcgtgtg aaatatcgtg gaattgcggt ggcggatacc
    13561 gctcgtgagc gcccggtgtt gaccgtacag ctgctggaca aacagccgcg cttaacggtg
    13621 agcacgattg aagataaacg tcaggcatta ctggcggggc ttggcgtggc gacgatgccg
    13681 tatccgatgg tcgaaaaaga tattgcggaa gggcggttgc gtgtcgtcag cccggaatcg
    13741 accagcgaga tcgatattat tatggcctgg cgtcgtgaca gtatggggga agcgaaatcc
    13801 tggtgtctgc gggaaattcc caaacttttt agcggaaaat aatatttgtt attgttaata
    13861 aaaaggggcg aaatatcgcc ccaattctaa gattaatttg aaccaaactt cggatcgttt
    13921 ccgtaacgat tgctgccaga agtaccttct gtgcagaaaa aaaccagaag cacgagccag
    13981 ccaataaacg gaacaaaaaa gagcaaggcc caggcacctg aacggtcggt atcgtgtaag
    14041 cgacggatag caagcgcaag gacgggaagg aaggttgcca gcaaataaag catagacaga
    14101 tatggaagct ccagacctaa aatcaattga atgacattga taatagcgcc gacgatggcg
    14161 ttaatcagag taaacatcca gtactcttta cgacgtgcgc gaccagagaa accaacataa
    14221 ttttttaata cggataaata ccactgcata aaaacatccc tttaattaaa agtg
//
LOCUS       DAPGRN010000118        14146 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPGRN010000118 DAPGRN010000000
VERSION     DAPGRN010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.120"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            164..1222
                     /gene="citC"
                     /locus_tag="RHW33_003812"
     CDS             164..1222
                     /gene="citC"
                     /locus_tag="RHW33_003812"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV1647271.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTKG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1237..1533
                     /gene="citD"
                     /locus_tag="RHW33_003813"
     CDS             1237..1533
                     /gene="citD"
                     /locus_tag="RHW33_003813"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV1647272.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1530..2438
                     /gene="citE"
                     /locus_tag="RHW33_003814"
     CDS             1530..2438
                     /gene="citE"
                     /locus_tag="RHW33_003814"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV1647273.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2449..3981
                     /gene="citF"
                     /locus_tag="RHW33_003815"
     CDS             2449..3981
                     /gene="citF"
                     /locus_tag="RHW33_003815"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV1647274.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQNGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3985..4536
                     /gene="citX"
                     /locus_tag="RHW33_003816"
     CDS             3985..4536
                     /gene="citX"
                     /locus_tag="RHW33_003816"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV1647275.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCAQSAAVCARGKT
                     HQLTDLLNRMEALLNYVDACNVN"
     gene            4511..5389
                     /gene="citG"
                     /locus_tag="RHW33_003817"
     CDS             4511..5389
                     /gene="citG"
                     /locus_tag="RHW33_003817"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV1647276.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5440..6903
                     /gene="citT"
                     /locus_tag="RHW33_003818"
     CDS             5440..6903
                     /gene="citT"
                     /locus_tag="RHW33_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV1647277.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPKATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7017..7823
                     /gene="rna"
                     /locus_tag="RHW33_003819"
     CDS             7017..7823
                     /gene="rna"
                     /locus_tag="RHW33_003819"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV1647278.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEMQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8053..8463
                     /gene="rnk"
                     /locus_tag="RHW33_003820"
     CDS             8053..8463
                     /gene="rnk"
                     /locus_tag="RHW33_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV1647279.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8694..9932)
                     /locus_tag="RHW33_003821"
     CDS             complement(8694..9932)
                     /locus_tag="RHW33_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV1647280.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10153..10581
                     /gene="uspG"
                     /locus_tag="RHW33_003822"
     CDS             10153..10581
                     /gene="uspG"
                     /locus_tag="RHW33_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV1647281.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="RHW33_003823"
     CDS             complement(10702..12267)
                     /gene="ahpF"
                     /locus_tag="RHW33_003823"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV1647282.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="RHW33_003824"
     CDS             complement(12396..12959)
                     /gene="ahpC"
                     /locus_tag="RHW33_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV1647283.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13331..14077
                     /gene="dsbG"
                     /locus_tag="RHW33_003825"
     CDS             13331..14077
                     /gene="dsbG"
                     /locus_tag="RHW33_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV1647284.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3353 a         3748 c         3566 g         3479 t
ORIGIN      
        1 ttcacgccaa aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatca
       61 cccccctttc tcccaagtga aaataaaagg ttatcagttt gcaacattga acaacattcg
      121 ttgcaaatcg ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat
      181 tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca
      241 tgaaaatgat ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga
      301 aaagcttatc gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga
      361 atccgttcgc ggtgaaggac tggcgctgac attagccact gaactgataa acctcgccta
      421 tgagcggcac agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg
      481 ccagtgcggt ttttccacgt tgaccagcgt accaggcgtg atggtgctga tggaaaacag
      541 cgccacgcga ttgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc ccgggaacaa
      601 gattggctgc attgtgatga acgccaatcc ctttacgaag ggtcaccgtt atctgattca
      661 acaggctgcg gcacagtgcg actggttgca tctgttttta gtcaaagaag attcttcacg
      721 cttcccctat gaagaccggc tggatctggt gttaaaaggc accgccgata ttccacgcct
      781 gactgtgcat cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat
      841 taaagaacag agcgtcatta accattgtta caccgaaatt gatttgaaga ttttccgtca
      901 gtacctcgct cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg
      961 cgttaccgcc cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc
     1021 accgcccatc gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc
     1081 ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc
     1141 tgcagtcacg ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc
     1201 tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt
     1261 gcaggcaccc ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat
     1321 atcgacctgc aaatcaatag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc
     1381 attctggacg ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa
     1441 ggcgcactgg actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt
     1501 atcccggctc tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa
     1561 ctcgcacccg ccgcagcatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact
     1621 ccttcatcta cccggctgat gccctgatgt ttgacctcga agactccgta gcattgcgtg
     1681 aaaaagacac cgcccgccgc atggtttacc acgcgctgca acatccgctg tatcgcgata
     1741 ttgaaaccat tgtgcgtgtc aacgcgctgg attccgaatg gggtgtcaac gacctggaag
     1801 ccgtcgttcg cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg
     1861 ttctggatat tgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca
     1921 gcaccggcct gctggcggcg attgaatctc cgctgggcat tacccgcgca gtggaaatcg
     1981 ctcacgcttc cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc
     2041 tgcgtacaga acgctccccg gaaggaactg aactgctgtt cgcacgctgt tccattttgc
     2101 aggccgcgcg ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg
     2161 aagccggatt cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc
     2221 tgatcaaccc gcgtcagatt gatctgctgc acaacctcta cgcaccgacc cagaaagaag
     2281 tggatcacgc ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg
     2341 tggtttccct gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc
     2401 tctcccgtgc agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa
     2461 attgaacaat ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat
     2521 cttgccgctt tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc
     2581 aaactgtgcg ccaacctgga agaagcgatt cgtcgctctg gtttacagaa cggcatgacg
     2641 gtttccttcc atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc
     2701 atcgcgaaga tgggctttaa aaacctgacc ctggcgtcca gctccctgag tgattgccat
     2761 gcgccgttgg tagaacacat tcgccagggc gtggttaccc gcatttatac ctccggcctg
     2821 cgcggtccac tggcggaaga aatctcccgt ggtctgctgg cggaaccggt gcaaatccac
     2881 tctcacggcg gacgtgtgca tctggtacag agcggcgaac tgaatattga tgtggctttc
     2941 ctcggcgtcc cgtcatgtga tgaattcggt aatgccaacg gctacaccgg taaagcctgc
     3001 tgcggctccc tcggctatgc aatggttgat gccgacaacg caaaacaggt cgtgatgctt
     3061 accgaagaac tgctgcctta tccgcataat ccggcaagca ttgaacaaga tcaggttgat
     3121 ctgatcgtca aagttgatcg cgttggcgat gctgcaaaaa tcggcgctgg tgcgacccgt
     3181 atgaccacta acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgtcaac
     3241 tctggctact tcaaagaagg tttctccatg caaaccggca ccggcggcgc atcgctggcg
     3301 gtaacccgtt tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc
     3361 ggcggtatta ctgcaacgat ggttgaccta cacgaaaaag gtctgatccg caaactgctg
     3421 gatgtgcaga gctttgacag ccacgctgcg caatcgctgg cccgtaaccc caatcacatc
     3481 gaaatcagcg ccaaccagta cgctaactgg ggttcgaaag gcgcatcggt tgatcgtctc
     3541 gacgtggtgg tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc
     3601 ggctctgacg gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcctct
     3661 gcgctttcca tcatcgtcgc gccgctggta cgcggtcgta ttccgactct ggtggataac
     3721 gtgctgacct gcatcacccc aggctccagt gtcgatattc tggtcacaga ccacggtatc
     3781 gcagttaacc cggcacgtcc ggaactggca gaacgtctgc aggaagcggg cattaaagtg
     3841 gtttccattg agtggctgcg cgaacgcgcg cgtctgctga ccggtgaacc acagccgatt
     3901 gaattcaccg accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt
     3961 gtgcatcagg tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg
     4021 gtatcaattc ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgcctgg
     4081 ctcaagcgcc atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa
     4141 gacagcgagg tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc
     4201 acaaagcagg gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag
     4261 ggcatgttga gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa
     4321 catagtcatc ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgaaatt
     4381 ctctcccgcc gcgactattc actgccgcct cgccgctgcc tgttgtgcgc gcaaagcgca
     4441 gctgtttgcg cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggca
     4501 ctgctgaact atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt
     4561 aatcgatgag tacgccctgc tgggctggcg cgccatgctg actgaagtca atctgtcacc
     4621 gaaaccaggc ctcgtggatc ggattaactg cggtgcgcac aaagatatgg cgctggaaga
     4681 tttccaccgc agcgcgctgg cgattcaggg ctggttaccc cgtttcattg aatttggtgc
     4741 ctgtagtgca gaaatggcac cagaagcggt actccacggg ttacgcccaa ttggtatggc
     4801 ttgcgaaggt gatatgttcc gcgctactgc gggcgtaaac acgcataaag gcagcatttt
     4861 ttctttaggg ctgctatgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac
     4921 gccaacaacc gtttgttcta cggcggcaag tttctgccgt ggcctgaccg atcgcgaact
     4981 gcgtaccaat aattcacaac tgacggcagg tcaacggttg taccagcagc ttggccttac
     5041 cggcgcacgc ggtgaagccg aagcgggtta tccactggtg atcaaccacg ccttgccgca
     5101 ttacctcact ctgctggatc aggggttaga tcctgaactg gcattgctcg ataccttgct
     5161 cctactgatg gcgatcaacg gcgataccaa cgttgcatcg cgcggtggcg aggggggcct
     5221 gcgctggcta cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc
     5281 cgatctcgat tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg
     5341 cggcagtgcc gacctgctga tccttacctg gtttttagca cagatttaat tatttaagca
     5401 cttgataaat tttgaaatat taattttcgg agaacccgta tgtctttagc aaaagataat
     5461 atatggaaac tattggcccc actggtggtg atgggcgtca tgtttcttat ccctgtcccc
     5521 gacggtatgc cgccgcaggc atggcattac ttcgctgtgt ttgtggcaat gattgtcggc
     5581 atgatcctcg agccaattcc ggcaacagcg atcagtttta ttgcggttac catttgcgtt
     5641 attggcagta attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg
     5701 caaaaacagg cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta
     5761 tttggcgcat ttatttttgc attagggtat gaagtttccg ggttaggtcg tcgcattgcc
     5821 cttttcctgg tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt
     5881 atcgacattc tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt
     5941 tttccggtca ttaaaaacct gccgccgctg tttaaatcat tcccgaacga tccgtccgcg
     6001 cgtcgtattg gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc
     6061 atgtttgtca ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc
     6121 ggtatccaga ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg
     6181 cttatcattg cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa
     6241 gaagtggcaa cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac acgcagagag
     6301 tggacgctga ttggccttgt attgctcagc ttaggtttgt gggtatttgg cagtgaagtc
     6361 attaatgcta ctgcggttgg tctgctggca gtttcgctaa tgctggccct gcacgttgtg
     6421 ccgtggaaag acattacccg ctataacagc gcatggaaca cgctggtcaa cctggcaact
     6481 ctggttgtga tggctaacgg cctgactcgt tctggtttta ttgactggtt cgccggtacc
     6541 atgagtacgc acctggaagg attctcacca aaggcaacgg tgattgtact ggttctggtg
     6601 ttctactttg cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg
     6661 gttattctgg ccgtcggtaa agggatcccg ggcgtaccaa tggaacaact gtgtatcctg
     6721 ctggtgctgt ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg
     6781 attatttacg gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc
     6841 ggggtgattt acatctctat gttgctgttg gttggctggc cgattctcgc catgtggaac
     6901 taatacgttc gccaaacgct gaataaatca tcacgcccgc caggtaagcc cacctggcgg
     6961 gcttttttat gatttaatag atagtattca gtgtatgtat gagttccaca cccattatga
     7021 aagcattctg gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca
     7081 acgccgtagc gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct
     7141 cctggcaaac cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat
     7201 gtcgtctgca aaccgaaacg accaacaaag ctgattttct gaccgtacat ggtctgtggc
     7261 caggattgcc taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg
     7321 gttgcgctac tcgcccaatc ccgaatctac cagaagcacg cgccagccga atgtgttcat
     7381 cgccggaaac cgggttatca ctggaaacgg ccgctaaact aagtgaagtc atgccaggag
     7441 ctggcggacg ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt
     7501 ttgatccgga cgcatacttc ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg
     7561 aagccggaaa attccttgcg gataattacg gtaaaacagt gagccgccgt gactttgacg
     7621 ccgcctttgc caaaagctgg ggaaaagaga acgtgaaagc agttaagcta acgtgccagg
     7681 gtaaccctgc gtatttgact gaaatgcaga tctcgatcaa agctgacgcc atcaacgctc
     7741 cgctttctgc caactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg
     7801 tgattgataa agcgggttat taatcatctg atttacaaaa cacgctaatt cgtcttccat
     7861 aaaccaaggc gattaagatt tatacataat gcgctgcatt gaggaattcc ctgaatgtga
     7921 cgcaaatcac ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc
     7981 gcctgacggt gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga
     8041 catggagtaa aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc
     8101 gatattctgc tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca
     8161 gagttggatc gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg
     8221 aacagccggg ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg
     8281 tatccggcaa aaatgaccga tagcaatact cagctttccg ttatggctcc ggtaggtgcc
     8341 gcactgctgg ggctgcgcgt tggcgattct attcactggg aacttccggg cggcgttgca
     8401 acccaccttg aagtgctgga actcgaatac cagccagaag ctgctggcga ctatctgctt
     8461 taatcccatc tgcacaagcg ataactcgct ttgcccagag gatgcactcg catcctcttt
     8521 acgcaaaatc agcaattttt cctggttctc actgttcact tttgctgcaa aattattccg
     8581 tttcgtttgt gggaataacc atggcagaag cattttacat cctgatagga tttctgatta
     8641 tggcggcgat tatcgtcatg gctgtccttt acctcgaaaa ccactcctga cgatcatatt
     8701 gttccccccg gcatcgcatt caccagacct gaaaccgcct tctgcgccgc ctctgcgctt
     8761 tgtgcaccgg gtaccagaat caccttacgg cactcctctt cacgtttttc gaaaatctca
     8821 tatccccggg cggcctcttc aaacggcata tagtgggtaa caatttcttc tggtttcagt
     8881 aatcctttct caattaacgg taataattct cccagccatg cgtgaacgtg ggtctgtccc
     8941 attttaaacg tcaacccttt atcaaaggcg tcgccaaaca ggaaaccgtg aataaatcca
     9001 gcgtagacgc ccggtacgct aacaatgccg ccacgcctga ccgccgcaat acactggcgc
     9061 aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc cgtggtgctg
     9121 cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg acctgccgtt
     9181 tgttcaataa ttgactgtgc cggatcgcta tcttcatcaa aattaatcgg gatcgcgccg
     9241 tagcggtcgg cggcgaaacg caagcggtag ggatgatgat caacaacaaa aatctgttcc
     9301 gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccggc accatagact
     9361 gcaacgcttg aaccttgttg gatctgcgca ttttttgctg cctgccatgc cgttggcaga
     9421 atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt aaacggcccc
     9481 acattccctt tagggacgcg gacatattcc gcctgcccgc caggaacgcc gccatacagg
     9541 tgactataac caaacaatgc cgctggagct ggtatctgtt ttttattgag cgcagcgcct
     9601 ttacccgcat tggtattttc gcaggcggca tattgctgta atcgacagaa aaaacagtcg
     9661 ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt ttttacgtcc
     9721 tttccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc atgtttaacc
     9781 tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt tgccgtaata
     9841 cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc tacctgaacg
     9901 tgatgtgggc cgtgataagt caatgctttc atgcaacctc catagacagt tcaaaaaaga
     9961 gtcagtcttt aagggtagct atcacagcgg gaaaacgggc agtaccacag gttttgggag
    10021 aattcctggt gttcagtgag aatatattca tcacagaaac agttcgtaag gaaaaagaac
    10081 cagacaaata tcagggataa atattataat catggcgaat gatttgttca tgattaacag
    10141 ggagaaaggg ttatgtataa gacaatcatt atgccagttg atgtatttga aatggaattg
    10201 agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt tattcatcta
    10261 cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc tgatgtgcgt
    10321 cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat ggtcagccac
    10381 ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt gcgggatgaa
    10441 gtcaatgagt tggcagaaga actgggggct gatgttgtag ttattggttc tcgcaaccca
    10501 tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca cgccaatctg
    10561 ccggtgctgg ttgtgcgtta attgccagaa taagtctcca gccctgcccg gaggcattcc
    10621 ggcggggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg ctaagcaatt
    10681 gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt caaaagcact
    10741 cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg gaaccgtcgt
    10801 acagtcaccc gctgcgaaca cgcctttgac gttggtttcg catttcgcat caatgataat
    10861 ctcgcccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg gcagcagacc
    10921 aatctggacg aaaataccag ccagttcgat gttgtgaata tcgccgctga cacgatcacg
    10981 atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtagttt gcgcattcag
    11041 aatgatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt cggctttcat
    11101 ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt cgattgccgc
    11161 ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa acagcggacc
    11221 gtcgcagtgc gggcagtagg tcacgccttt ggtgcgatac tgatcttcgc ccggaacgtt
    11281 catgttgcgc cattttgcac cggtcgccac gataatgctg cgtgctttca gtaccgcgcc
    11341 agaagctgtt tcaatctgat gcagaccacc ttcaactgct gctgggatca gtttgctggc
    11401 gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca gtgcgcctgc
    11461 caacttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa cggtatcgag
    11521 gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac gtgcggagta
    11581 aattgctgcc gctgcacccg ccgggccgga accaacgatt aatacgtcat aagcatcacg
    11641 cttgttcagc tcttctgccg cacgtttttc cgcgccagta tcaattttgg caacgatttc
    11701 agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg ccggaacgcc
    11761 catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg cagtgtgctt
    11821 gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg ggcagttatg
    11881 gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc gaatctgctc
    11941 cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagtaacg ccagtaccag
    12001 cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggcccctggt tggaacctgg
    12061 gttggtgatc aggaaagacg gcttacgcac cggcaagctg ttatcttctt taaaggtgac
    12121 tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg atttagcgct
    12181 gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt aagccttgag
    12241 ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca tcatcatgca
    12301 agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag aaaatcgggt
    12361 gccgcagcac ccgattaatt acgcgattgc ggaacttaga ttttaccaac caggtccaga
    12421 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    12481 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcatc acggccaatg
    12541 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    12601 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    12661 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    12721 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
    12781 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    12841 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    12901 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    12961 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa
    13021 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    13081 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    13141 atctgaacta ccctttggta tcttcagcgc ctgcctgcag gctgaggtgt tgcccttaca
    13201 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    13261 gctcttacct atgacaatga taggttatgc cttttactcg acttttgcac tgactgaaaa
    13321 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    13381 agaggaactt cctgctccag taaaagcgat tgaaaaacag ggtattacga tcatcaaaac
    13441 gttcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
    13501 catctacctg actccagatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
    13561 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
    13621 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagacg cgccggtcat
    13681 tgtctacgtc ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
    13741 cccgtgggta gattctggca aagtacagtt gagaacattg ttggttgggg ttatcaaacc
    13801 agaaagtccg gcgacagccg cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    13861 agaatatgaa gcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
    13921 gcaaatgaaa gtgttaagcg acaacgagaa actcatggac gatctggggg caaatgtcac
    13981 tccggctatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
    14041 tcagaaaacg cttaatatca ttatgggaaa taaataaaag gtaattcatg gatggttgaa
    14101 ctatatccct gcttttcaac catcctaaaa aagcatgaat tgctcg
//
LOCUS       DAPGRN010000119        14134 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.121, whole genome shotgun sequence.
ACCESSION   DAPGRN010000119 DAPGRN010000000
VERSION     DAPGRN010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.121"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(259..828)
                     /locus_tag="RHW33_003826"
     CDS             complement(259..828)
                     /locus_tag="RHW33_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647285.1"
                     /translation="MGKNNHPENKYREWFSTAITYLIAGGLCWYMLWASAISHYTVLQ
                     NTVPEWTTYFCTGITTGSLSFIYAFYIRTFNRTLKYLLNAFTGGFCLGFILSLNCYDV
                     CVYLFPDKVISYESEYDVVFPGPSRGKYGHCEAGLWLKDQNTSRWIQLCTNKEFLRSH
                     HKQGMTGVWVTARVNKIGSYIVKYEFIYM"
     gene            complement(929..2161)
                     /gene="dgt"
                     /locus_tag="RHW33_003827"
     CDS             complement(929..2161)
                     /gene="dgt"
                     /locus_tag="RHW33_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HDV1647286.1"
                     /translation="MYCNELKEFAQEQEQKLISALADTREHPSPDGETRSRDNYQRDY
                     ARILYSSSFRRLQGKMQLFEIDPEKFNRNRLTHSLEVAQIARSIASDLKLINPVVVEL
                     AALAHDIGNPPFGHSGEKLLNELSEEIGGYEGNAQALRILRKLEKKFSYCNGLNLTHR
                     SLLSVVKYPIPRAAATAGKFIYDDDYYFYINLLAENQLDLNPGEKTIDAQIMDLADEI
                     AYAAHDLEDALSRNMVTIEDIEYEFQISDEFRGAREQFREIVTQSRNTAFQANLLKTS
                     EEFAIIFRKELTSNIVNRLVADISVVTNLNGFQELGFGKLNALSEGLKKLLFKVIMRK
                     RNILTYEFRGNKIIRDLYDFYNEGENYKFLPPELKFTLPQPDSCIFKISKKRAVVDYI
                     SGMMDTFAVKEWETHCLK"
     gene            complement(2312..3473)
                     /locus_tag="RHW33_003828"
                     /pseudo
     CDS             complement(2312..3473)
                     /locus_tag="RHW33_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3641..4621
                     /locus_tag="RHW33_003829"
     CDS             3641..4621
                     /locus_tag="RHW33_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV1647287.1"
                     /translation="MTLLVTAECVDDLINDVHQAILADGLSNDPTKGSTLEILGAYLV
                     LTDPRRRISRTESRGKLISCLGEFLWYLSAQNDLEFIKHFIPRYAESAESDGKIHGGY
                     GPRLFNMRGLYNQLKNVINLLQEKKPTRRAVIQLFDASDLVPSKTEQREYVDIPCTIS
                     MQFIVRCEELHLFVCMRSNDAFMGLPHDVFAFTMLQELVARLIGCELGHYHHFVSSLH
                     LYKNDLEKVKALQKEGFMSTLPVMGSMPEMHSLDDILPLLEAEKALRENKEFDIDTTS
                     LNDYWKDLLRILKIHFLLRSHIPNHKGLAFQELAAMKNQEYRFIFEGRLQ"
     gene            4618..5562
                     /locus_tag="RHW33_003830"
     CDS             4618..5562
                     /locus_tag="RHW33_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647288.1"
                     /translation="MKRHNQISQLVSNLQNFSRNEHNLNGLSSPACFDVLACQIIDSI
                     RRIRYVETLALRTDYMTPLRKEPNSDVFDPLRAACLYLRDNNYDEACWLVFLATHFGK
                     SNKTGWILCRDIYSGLGTQTWTWDTITDDFAAFEQWFASVSDELTANSSLRQYGNHRK
                     YETKKYHSRRSIPAVFRSYIGFIGATHSHEARFAEAKSFSSSPESLFELLYSGLNAVI
                     SFGRTAKFDYLTMLKKTGLLDVEPGHAFLNGATGPLQGSRLLFSNSRTAGDTIDVLNE
                     KLADLAAIIPAPYLRMQVIEDALCNWQKSPDRYVYFGG"
     gene            complement(5565..6647)
                     /locus_tag="RHW33_003831"
     CDS             complement(5565..6647)
                     /locus_tag="RHW33_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDV1647289.1"
                     /translation="MIVTQDFQPDWVSPPGDTIIDLMDEHGLSGEELSKRIGLSLIRG
                     QQLLEGKIRLNESLACKLEELFNVSTDFWIKREVAYRQQIGYLEKVNNDWLESLPVRD
                     MMEFGWIPKTTTRESKFQHCLSFFGINSVNDFFYKIQSDAPLVAFRKSLNLATEPMAD
                     LVWLTRAAQLTSNQNCAVWDKEVLIALLPELRKATNEPDMRIFIPYIRDLLATAGVSL
                     VVLPTPKGCRASGATCFFTPDRATLVMSFRYLTDDHFWFTLFHEIGHLILHEAVRVRV
                     EGKDSLSSEEEKEADKFAVDVLIPEEYRQQLSRYGVRHWKEIIRLARKMGVSKGIVLG
                     YLQHEGNIPFSHLNRFKVRFRKEDIV"
     gene            7114..7377
                     /locus_tag="RHW33_003832"
     CDS             7114..7377
                     /locus_tag="RHW33_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDV1647290.1"
                     /translation="MKGKHQDTKALSDVLAEMQRQDAKWGADRNQDPFIWGAILGEEV
                     GEFHQAVLHDRFGGKAAGTSREEAVQIAAVALQIIEYYDRVID"
     gene            complement(7679..8193)
                     /locus_tag="RHW33_003833"
                     /pseudo
     CDS             complement(7679..8193)
                     /locus_tag="RHW33_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8801..12214
                     /locus_tag="RHW33_003834"
     CDS             8801..12214
                     /locus_tag="RHW33_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV1647291.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDSRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGQRINQLRD
                     LGWWKQASLKELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDSVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            12278..13912
                     /locus_tag="RHW33_003835"
     CDS             12278..13912
                     /locus_tag="RHW33_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV1647292.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSKNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            13909..>14134
                     /locus_tag="RHW33_003836"
     CDS             13909..>14134
                     /locus_tag="RHW33_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV1647293.1"
                     /translation="MKKNQLVTLGKHIDILSGCAFPSSGFNRNNGVPLIRIRDILSGK
                     TETYYEGSYDLKYLIKKGDLLVGMDGDFNRE"
BASE COUNT         3871 a         3312 c         3276 g         3675 t
ORIGIN      
        1 atagtgataa gagccagtca gcgagactga ttgttttatc tgtactggca gcaggcagtt
       61 taccatggct tactgcttca gcttctgaat ctgtgttgat gatatttatc tgtttataag
      121 gcaaggcttg tctctgtgag ttggtaatct ttatcccggg atagcttttc tctgtatttt
      181 tagtatagcc agtaatggat tactcctgta tgcgcaaaaa tatagcaact catccatggt
      241 aaacatgtca gcttttacct acatatagat aaattcatat ttcacgatat acgacccaat
      301 tttattaaca cgagctgtta cccatacacc tgtcatcccc tgtttatggt gactgcgcag
      361 gaactcttta tttgtacaaa gttgtatcca tctggaagtg ttttgatctt tcaaccagag
      421 tcccgcttca caatgtccat actttcctct tgatggtccc ggaaaaacaa catcatattc
      481 agattcatag ctaataacct tatccggaaa aagataaaca cagacatcgt aacagttcag
      541 actcagtata aatccaagac aaaatccccc cgtgaatgca tttaataaat actttaatgt
      601 tctgttaaag gtgcggatat aaaatgcata aataaaagaa agagaaccgg tggttatacc
      661 ggtgcaaaaa tatgttgtcc attcaggtac cgtattttgt aaaacagtat aatgagatat
      721 ggcggatgcc cacaacatat accagcacaa accacctgca ataagatatg ttattgctgt
      781 ggaaaaccat tccctgtatt tattttcagg gtgattgttc ttgcccatca acgcatttcc
      841 agaatattta aaaataacct gtcaccgaaa cagaataaag gcgcaataac cataacggga
      901 aaatatgcgc ctctggtaat cgacagtgtt acttcagaca gtgagtttcc cattccttga
      961 ctgcaaatgt atccatcata cctgaaatat agtcaaccac tgctcgtttt ttgctgattt
     1021 taaatataca ggaatctggt tggggtaagg tgaatttaag ttcaggaggc agaaatttat
     1081 aattctctcc ttcattgtaa aagtcatata aatccctgat tattttattt cccctgaact
     1141 cataggtgag gatattacgt tttcgcatga tgactttaaa gagaagtttt ttgagccctt
     1201 cggataacgc attcagtttc ccgaatccca gttcctgaaa accattcagg ttcgttacta
     1261 cagaaatatc tgcaacgagg cgattgacaa tatttgaagt taactccttg cggaatatga
     1321 tggcaaattc ttctgaggtt ttcagtaagt tagcctgaaa agcggtgttt ctcgactgag
     1381 taacgatttc cctgaactgt tccctcgctc cccggaattc gtcagaaatc tgaaactcat
     1441 attcaatatc ttcaatcgtg accatgttcc tgctaagggc atcttccagg tcatgcgcgg
     1501 cataggcaat ctcatccgcg agatccatta tctgcgcatc aatcgttttc tctccgggat
     1561 tcagatcgag ctgattttca gcaagcaggt taatgtaaaa atagtaatca tcatcataaa
     1621 tgaacttgcc ggcagtagct gcagcgcggg ggatgggata cttaacaacc gaaagtaaac
     1681 tacggtgagt cagatttaat ccgttgcagt atgaaaattt cttctccagt tttctcagga
     1741 tacgtagcgc ctgtgcatta ccttcatagc cgccgatttc ttcagacagt tcattaagca
     1801 gtttttcacc ggaatgaccg aaaggaggat ttccgatgtc atgcgccagc gctgccagtt
     1861 caaccaccac cgggttaatc agtttgaggt ctgacgcgat acttcgggcg atctgagcaa
     1921 cctcaagact gtgcgttaat ctgtttctgt tgaatttttc aggatcaatt tcaaaaagtt
     1981 gcatctttcc ctgcagcctg cgaaatgatg atgaatacag aatacgcgcg taatcccgtt
     2041 gataattatc ccggcttctt gtttcaccat cgggggacgg gtgctctctg gtatcagcaa
     2101 gcgcactgat aagtttttgt tcctgctctt gcgcaaattc ttttaattca ttgcagtaca
     2161 tatttcttcc tttctggagc atgttaccaa atcagaatac cggatgaaca aaactggtgg
     2221 ctacatcctg tagggattat gtattagtaa gctctggcca ttagtgccat ttcaggttat
     2281 gtgatcctac ccacgtaata tggacacagg cctaagcgag gttcttgttt tcaaattgtt
     2341 ccggactgag gccgccacac caactgtgcc gccgccaccg attgtaatca cattcgatat
     2401 aattaaacac cgttgcccgc attatttccc ggctgataaa gtgttctcca tggatacatt
     2461 ccactttcag cgaatgaaag aagctttcca cgcaggcatt atcgtagcag caaccttttg
     2521 cgctcatact tccacgcaga ttatgccgct tcagttgcgc ctgataatct gctgaacagt
     2581 actggcctcc acggtccgtg tgaacgataa cgttccgggg cctcttacgc cgccacagcg
     2641 ccatctgcag ggcatcgcag gccagttgcg ccgtcatgcg tggcgacatt gaccagccaa
     2701 taacggcacg tgaccacagg tcaatgacca ctgccagata cagccagcct tcatctgtac
     2761 gtaagtacgt gatgtctcct gcccacttct ggttcgggcc actggcgtaa aaatcctgct
     2821 ccaacagatt ttctgacaca ggcaggccgt gtgcgcggta gctgaccggg ctgaacttcc
     2881 gggaggcctt tgccctcagt ccctgacggc gcaggcttgc cgccacggtt tttacgttaa
     2941 aggggtaacc ctgagcacgc agttcatccg tcaggcgtgg ggcaccgtaa cgctgttttg
     3001 accgggtaaa agccgcgagg acaacgctgt cgcagtgttg gcggaactgc tgacgcgtgc
     3061 ttatccttgt ccgccgctga caccacgtat accagccgct gcgggccacc cggagcacgc
     3121 ggcacattgc tttgatgctg aactcagcct gatgtttttc aataaagaca tacttcattt
     3181 cagacgattc gcaaagtatg tcgcggcctt ttggaggata gccagttcct ctgcctgctc
     3241 cgccagttgc cgcttcaggc gggcattttc agcggccagt tcactttcac gctctgacga
     3301 actcatttgt tgctgcagtt tgctgcgcca cgcatagagc tgtgattcat acaggctgag
     3361 ttcacgggct gcggcagcaa caccgatgcg ttcagcaagt ttcagggctt cactgagaaa
     3421 ttcaggcgaa tgctgtttac ggggttttta ctggttgata ctgtttttgt catgtgagtc
     3481 acctctgact gagagtttac tcacttagcc gcgtgtccac tattgctggg taagatcaag
     3541 tcttacttac cctgtgaacc atgttgatat tgatactata ttgactatat gccatacagg
     3601 tggtaatgat tagttactac catacaggaa aaggtagaaa atgactcttt tggtaacggc
     3661 tgagtgtgtt gacgatctga taaacgatgt tcatcaagct atcctagcgg atggactcag
     3721 taacgatcct acaaaaggat caactctaga gattcttggt gcctatcttg tccttaccga
     3781 cccccgccga agaataagta gaacagaatc ccgaggaaaa ctaattagtt gcttaggtga
     3841 atttctgtgg tatctctcag ctcagaacga tcttgagttt ataaaacact tcataccacg
     3901 ttacgctgaa tccgctgagt cagatgggaa aatacatggt ggttacgggc cacggttatt
     3961 taatatgcgt ggtttatata atcagctaaa aaatgtcatc aaccttttgc aggagaaaaa
     4021 acctaccaga cgtgcagtaa tacagctttt cgatgcctca gacttggtgc cctcaaaaac
     4081 tgaacagcga gaatacgtag atattccttg tacaatatct atgcagttta ttgtcagatg
     4141 tgaagagctg catctatttg tttgtatgcg ttctaacgat gcctttatgg ggctgcctca
     4201 tgatgtattt gcgttcacga tgctacagga attagttgcc cgcttgatag gatgcgaatt
     4261 gggtcactat catcactttg tatccagcct acatctctat aaaaatgatt tagaaaaagt
     4321 gaaggctctt cagaaagagg ggttcatgag caccctacca gtcatgggat cgatgcctga
     4381 aatgcattcc ttagatgata tcttacccct gcttgaggca gaaaaagctc ttcgagagaa
     4441 taaggaattt gatattgaca ctacttcctt gaatgactac tggaaggatt tattgcgtat
     4501 attaaaaatc cactttttgt tacgttcaca tatacccaac cacaaaggac tagccttcca
     4561 agagttggct gcaatgaaaa atcaggaata tcggtttatt tttgaaggaa gactacaatg
     4621 aaaagacata atcaaatcag tcagctggtt agtaatttgc aaaattttag ccgcaatgaa
     4681 cacaacctaa atggcctaag ttctcctgcc tgctttgatg ttttagcctg tcagattatc
     4741 gacagtatac gtcgtatacg ctatgtggaa actcttgcat tacggaccga ttatatgact
     4801 ccgttacgaa aagagccgaa ttctgatgtt tttgatcctc tccgcgctgc ctgcctttat
     4861 cttcgtgaca acaattacga tgaagcctgc tggctggttt tccttgccac acatttcggc
     4921 aagagtaaca aaaccggctg gattttgtgt cgagatatat acagtggact cggtacacaa
     4981 acgtggacat gggatacgat aactgatgac tttgctgctt ttgaacaatg gttcgccagc
     5041 gtctctgatg agttgacagc taacagtagc ctaagacaat atggtaacca tcggaaatat
     5101 gaaacgaaga aatatcactc tcgtcgttca atacctgcgg tttttcgctc ttacatcgga
     5161 ttcattggtg ctacacacag tcatgaggct aggtttgcag aggccaagag cttttcatct
     5221 tcaccagaat ctttattcga actattgtat tcagggctaa atgccgtgat ctcatttgga
     5281 cgaaccgcaa aatttgatta cctaactatg ctcaagaaaa cgggacttct ggatgtagag
     5341 ccgggtcatg cttttctgaa tggagcaacc gggccccttc aaggaagccg gctacttttc
     5401 agcaatagcc ggactgctgg tgacacaata gacgtattga atgaaaaact agctgacctt
     5461 gcggcaatta ttcctgcacc ctacttgaga atgcaggtta ttgaagatgc gttgtgcaac
     5521 tggcagaaat cacctgaccg ttatgtctat ttcggcggct gatatcagac gatatcttct
     5581 ttacggaatc tcactttaaa ccgattaagg tgagaaaaag gtatattacc ttcatgctgc
     5641 aaatatccca gtacgatccc tttggatacc cccattttcc tagctaaccg gatgatctct
     5701 ttccaatgcc ggacaccata gcgactaagt tgctgccggt attcttctgg gataagaaca
     5761 tcaacggcaa atttatctgc ttccttttcc tcttccgacg aaagactatc tttaccttct
     5821 acgcgaactc ggacagcttc atgcaagatg agatgcccaa tctcatgaaa cagcgtaaac
     5881 caaaaatggt catcggtcag ataacggaaa ctcatgacta gggttgcgcg atctggtgta
     5941 aaaaagcagg tagcaccact tgcccgacat ccctttggcg ttggcaacac aacgagagaa
     6001 actccagcag tggcgagaag gtcgcgaatg taagggataa aaatcctcat gtcgggttca
     6061 tttgtagctt tgcgcagttc tggcaacaat gctatcagca cctctttatc ccatacagca
     6121 cagttttggt ttgaggtaag ttgtgcagcc ctagtcagcc acaccaaatc ggccataggc
     6181 tcggttgcca gattaagaga tttacgaaaa gcgaccaaag gagcatctga ctgaatttta
     6241 taaaaaaaat cattgacgga atttattccg aaaaacgata ggcagtgctg gaatttactt
     6301 tcccgagttg tagttttggg gatccaacca aactccatca tatcacgtac aggcaatgat
     6361 tcgagccaat cattattcac tttctcgagg tatccaattt gctggcgata agcgacttcg
     6421 cgcttgatcc agaagtctgt cgatacgttg aataactcct caagcttaca agccagagac
     6481 tcattgagtc ggatttttcc ttcaagaagt tgctgacccc ggataagaga caatccaatc
     6541 ctcttactca gttcttcacc agaaagccca tgttcatcca ttagatctat aattgtatca
     6601 ccgggtggag atacccagtc aggctggaag tcctgcgtaa ctatcatttc acgtcaccta
     6661 tgaacactaa ccgtatccgg ctaactcggg accagttaat tgtattatct tgatttttgg
     6721 gctgaactgt attcccacag acaaatccca tttttacatc accgagaatg gtaattgtaa
     6781 tacactcttc cccatcctca aagcagacct ccggcgaacc tacgggcaac agtaatatgt
     6841 tgtctgtcac ggaaagatcg gaaagtcgcg agcgtagtct tgatgccact ttgtcaccaa
     6901 agatctcatt tgcctcatgc atactctcgc aaatagtcct taacttcttg ctttgaaagg
     6961 taatgaacaa aattatccac ttttattttt cttggttgct acgtttatag caaactcaac
     7021 gctcattcac aaaatgagat gtagcgaatt aatggactct gcacatatta aatgttaatg
     7081 atggacttaa aatcacggat tcgtaaggga aacatgaaag gtaaacatca agacactaaa
     7141 gccctgtctg acgtgctagc ggaaatgcaa cggcaggatg ccaaatgggg ggcagaccgt
     7201 aatcaagatc cttttatctg gggagcgatt ctcggagaag aggtgggaga atttcatcag
     7261 gctgtcctcc atgatcgttt tggtggtaaa gcagccggta ccagtaggga ggaagcagta
     7321 caaattgctg ctgttgcact gcaaatcatc gaatattatg atcgcgtaat agattgataa
     7381 attagaatta atcaactaag agtcttttca cgcgaagatg aactctggca gccatctacc
     7441 ttcgtgggcc tgtagaacac atctaattgc aatactccca gttaatcact aaccagcctc
     7501 tccagtaagg ctggtcgatt aggtttaatt aaacagatat aacaagcaat tcacttggta
     7561 ttacgcggtt cacacagact tctttagttg tgtcatatcc tataactttc agaggcttat
     7621 ttacggtatt atcacgaacg gtgcaaaagt ggctcttccg caacctaggt ggagattttc
     7681 atgccagtgg actcattacc ctctgcctaa gtagttcaaa tccggctcga ccatacatca
     7741 gacgtttcag catcttcagg cgatttacat gaccttcgac aacaccgtta ctccagcggc
     7801 tgctggttgc ctcacatata gccgcagcat cagcttccat ccccgcagcc acgcgccgaa
     7861 gttctgctga accgttttcg tggactcgag tgaaccaact gcttagattt gatttatttg
     7921 gggttttcag aatacggtag aactcaagta ctagttgctg cgctattttc agcgccggtt
     7981 ctttttcaca catcagacta ataaatcggg aagcataatt ttcttcaccc ctgattatta
     8041 tccagggcat caaccagcgg cttacccggg acactgaagg aagtctggcg gcagtagtaa
     8101 ctggtgggtt ccagccttta cgccatttgg caacagcatc cctgacgatg gtttcactgc
     8161 ctgtaacccc cgggccacca tttcccgcca tatccagtcg ctggtgtgct gaattggtca
     8221 tgtatgaagg aagatatcaa tggtggtatc tccctttcaa aaggaagtga aaagcttgat
     8281 gcctgctgcc aggcaatcaa tattacgtgc ttttaacgac tatcctaaat ggtggtagct
     8341 atgcagagct cccggtccta gcgctgggag cttatcgctt atctttaccg taagcgtaaa
     8401 cggaccgccg tatgtagcca ttaaaccagt attggtaacg tagataccca ccttttcaaa
     8461 agggaagtgg ataaaagata gcgaaaatgc gaagagttga ttaatgcatt gaaacaaaat
     8521 tcattaatga tgaacttgga gaaaagctcc atcgttttat tggctcaaaa aacatatccc
     8581 atagatgatc agccatacct tcattggtta gcaaggtatc ctgtgagtag ccatggctga
     8641 agatgcccat tgatgggcga aaaccaagga tctgaccgtt agagcagtta tgtcgttaaa
     8701 cacatcacca ccatatgcca tccggctcac ttcgtcggcc aaattagtgt gatattcttt
     8761 ccagaaatgc tgttgtatcc acgacataag gcgatgattg atggagcagt ctctcaattt
     8821 tgaaatgttg cgcagccagt ggccggaact ggctgaactc gcgtgtatgg cggaacgcta
     8881 tgttcactcc gatccggaaa gttgtctggt caagctgcgc aactacaccg aattgatggt
     8941 gcgctggttg tatcgtcagg agcggttgcc ggaaggtatt aaggctaatc tttacgattt
     9001 aatgaatgct gatgtcttta ccagcatgat gccggaagcc atcatcatga aaatggatgc
     9061 gctgcgtatc catggcaacc gtgccgcgca cggcggacgt atcaaagcta aagatactta
     9121 ctggctgctc aaagaagcgt atttgttggg aatttggctg tatgttcgct acgcccacgg
     9181 taatgttgat gactgcccca aatttacact ccctccatta acacaatctt caggtcgtgc
     9241 agatgaaaaa cgtctggaag atgcaatcag ggctcaggat gaaagccgtg agcgcgaact
     9301 ggcgctgcaa cgcgcactac agcaagaaca ggaaaaggcc gaacacctca ctcaacgcct
     9361 gaatgaagcc agagcgcgta accagcatgt tgccgatatt ctctctattg atgaagcgga
     9421 aacccgccgt cgcctgattg actctcgcct gcttgctgct gactggaatg taggcgaagg
     9481 ccttaaaaat accgatcagg ttacgcagga acatcccgtt aaagaacaac ctaccgcgac
     9541 cggagacggt tatgcggact atgtcttgtg ggatgaggca cacaaaccgc tggcggtggt
     9601 ggaagcaaaa aaaaccagcg tcaatgccga gcaaggacga attcaggccc ggctgtatgc
     9661 agactggctg gaaaaagaat acggccagcg tccgatcatc ttctacacca acggctatga
     9721 tatctggctg tgggatgacc ataaaactca tggttatccc ccgcgtcggg tgtttggctt
     9781 ctacagcaag gaaagcctgc aatatcttat tcaacagcgt gaaacccgtc ttccgctgaa
     9841 cagtgtgccg cacgtaaaag ataacgaagg taaggccgtt gccggacgtt tgtaccagct
     9901 tgaaaccatt gcccgtgtca gcgagcggtt taccaacaaa taccggcaat cgctaattgt
     9961 tcaggctacg ggtacaggca aaacccgcgt ggcaatagcg ctaagcaaac tgatgattga
    10021 tgcccgctgg gtaaaacgcg tcctgtttct ttgcgaccgc aaggaactac gtaaacaggc
    10081 ggcgaatgcc tttaatcaat tcaccaatga gccgctgtat gtggtcggga aatcgaaaaa
    10141 agcagacagg cagaatgcta ggatctacat cgccacctat cctggtatga tgaaaatcat
    10201 ggaccatttc gatgttggtt atttcgattt gatcatcgcc gatgaatccc atcgctctat
    10261 ctacaacgtt tacggcgatc tgtttaagta ttttgatgcc ctccagattg gcctgacggc
    10321 cacgccgatt gacatggtga gtaaaaccac tttcggcctg tttggttgtg aaggacgtat
    10381 tcctaccgcc aactacagcc tggaagatgc catcgccgat aacaatctgg ttccctatga
    10441 ggttgtcacg cacaccacag aattcctgcg cgaaggcatc aaacgggaga aattaagcga
    10501 cgcacaaatc cgcgagctgg aagaacaagg catagatccc aatacgctgg agtttgatgg
    10561 caaggcgctg gatgaggcga tctacaacaa agacaccaac cgctatatcc tgcgtaacct
    10621 gatggaaaat ggcctgaaag atcgggacgg ccagttgcca ggtaaaacca tcattttcgc
    10681 ccgtaaccac aaacacgcgc tgctgttgaa tgaattattc gacgatatgt acccgcagtt
    10741 tgccgggcgc ttctgccagg tcatcgacaa ttacgattcc cgcgccgagc aactgattga
    10801 tgattttaaa gggctggatg aaagcaccaa caaagagctg accatcgcta tctccgttga
    10861 catgctcgat accggtattg atgtcccgga aattgtgaat ctggtctttg ccaaaccggt
    10921 caaatcaaaa gtgaagttct ggcagatgat tggccgtggt acgcgcctct gtccggggtt
    10981 gtacggttac gacgataacg gcaaacctct ggataaacag aaattccgca ttttcgatca
    11041 ctggggcaat tttgagtatc acgaactgca taccgaagag gccgaagtca cagcgacaaa
    11101 atcgcttgca caaaaacgct tcgaagcgtg ggttatgctg ggggccgctg cgcaacgtaa
    11161 attcgacaaa caggcggtgg atttagttgc tcaccagcta cgcgagcaaa tcaacgcact
    11221 ggatgaaaag tcgattgccg tccaggaaaa gtggcatcaa aaagcgcagt acagcgatga
    11281 aaaagtgctg cgccagcttt ccccgaaaac acagcaggat ttgctatctg tcctggcccc
    11341 gctgatgcag tggctggacg tgcgcgggca aagcgacgct atgcgttttg atatggatat
    11401 tctggcggcg caaactgccc gttataccaa cccggaagag ctggatgtgc tctggccggt
    11461 catcgtcgaa aaagtggagc gcctgccgcc gcatttggcg caggtgcaac agcagggaca
    11521 gcgaattaat cagcttcgtg atttgggctg gtggaagcaa gccagtctga aagagctgga
    11581 ggatatccgc attcatttgc gcggtatcat gcacctgatg gaaaaagacg cgacgccgaa
    11641 atttggttcg atacaggtgg atattaccga agatgcgaac ctgatccaga cggaaacccg
    11701 caaaaccaac atccgctcga ttgatttcaa actctatcgc cagcaagttc agggggcgct
    11761 ggagccgctg ttccagcaaa acccggttct gaagaaaatc cgcaacggcg agccagtcac
    11821 gcaaaacgag ctggatgaac tggcgaagct ggtgctgatc cagaacccca atgttgatat
    11881 ccgggcgctg aaagagttct atccacaggc aactgccagc ctggataaac ttttgcgtac
    11941 catcatcggg atggacagcg actcggtgga agtgcgcttt gctcagttcg ccgctgataa
    12001 cagtctgacc agccaacaac tgcgcttcct gtcattgctg aaaaaccaca ttcgcgatta
    12061 tggcaccatt gaaatgcggc agctctttga acagcctttt acgcatatcc acaacgaagg
    12121 cgttaccggt gtgttccctg atatagagca aatcgcccgc ttgcaaaaga tagtcgaaga
    12181 gctgggtgtt gtgaccgacg cagcgacggt ataatacgca catgacaagc cccggcagcg
    12241 gggcttttgt atttaaaaca ggatataaaa gctaacaatg atcactggtg acttaaaaag
    12301 taaaatcgac ggcctgtggg aagatttctg ggtgggtggt atcaccaacc cgctgaccgt
    12361 aatcgaacag atcacctatc tgatgtattc ccggatgctg gatactcagg aacaacgcga
    12421 cgagaagcgt aaacaaatcg cgggtattga ttttaaaccg cgttttgcac cagaacagca
    12481 ggagttccgt ttcagtcact atagcaatct tggctcggat gagatgatgg aagtggtgcg
    12541 cgatggtgta ttccagcatt ttcgtcagct cggccaggct gatgcgtcga aggtaacgct
    12601 gctgggcaac tttatgaaag atgcccgtct ggagattgtt aagccttcat tgctgaccaa
    12661 agcggttgag gtgatcaaaa acctgccatt ggatcgcggc gacaccaaag gtgaccttta
    12721 cgaatacctg ttaagcaagc tgaccactgc cggaatcaac ggccagtttc gcacaccgcg
    12781 ccacattatc cgcacgatgg ttgaaatgat ggagccgaac cccgcccgcg gcgagacgat
    12841 ttgcgatccg gcctgcggca ccggtggctt tctggcaacc agctatgaat atctactgga
    12901 gaagtacagc tcgctggaat ccattcatac cgagattggc accaacgaac gtggtgagct
    12961 ggaagagcaa aaaatcttta ccggcgatct gttgaccccg tggcgtaacc atgtggataa
    13021 caatatgttc cacggttacg actttgacac cacaatgtta cgtatcgccg ccatgaacct
    13081 gattatgcac ggcgtggaag cgccggatat ccactatcag gacaccatga gccagagctt
    13141 cagcaaaaac ttcccgcagg ccagtaaaaa cgctttcaac ctgattctgg caaacccgcc
    13201 gtttaccggt tcactggacg aagaagatat cgactccacg ctgtcggcaa tggtgaaaac
    13261 caaaaaaacc gaactactgt tcctggcgcg tattctgcaa atgctgaaag tgggcgggcg
    13321 cagtgccact atcgtgccgc agggcgtgct gtttggctcc agtaaggcgc accagtcact
    13381 tcgcaaaacg ctggtggaag ataaccaact ggaagcagtg atcaacctgc cttccggcgt
    13441 atttaaacct tacgctggcg tggcgacggc gatcctgatc tttaccaaag gcggtcagac
    13501 ggacgaggtc tggttctacg atctgcaaaa tgatggctac agcctggatg acaagcgtaa
    13561 cccaattaaa gacaacgacc tgccgcatct gctggcaagc tggaagcatt accgtacttt
    13621 acgcgggctg ccggttgata actttatggg tgagaagtta gcctcgttgc tgaaacagca
    13681 gtacccggaa gggattgatg ctggcgttga ttttaaagat cgcactcagg cggcgtttat
    13741 agtgccaaaa gaagatattg ctgcccagaa atacgatctc tctattaatc gttataaaga
    13801 agtggtgtat caggctgagg agtatgaaga tccgaaggtg atattgaagc ggttaaaaga
    13861 tctggaaaaa gagattctgg cggatctgga tgagctggag gggatgctgt gaaaaaaaat
    13921 caattggtca cacttggcaa acatatagac atattgtccg ggtgtgcatt cccttcaagt
    13981 ggattcaata ggaacaatgg tgttccacta atccgtatta gagatattct ttcagggaaa
    14041 accgaaacct attatgaagg cagttatgac ttaaaatatc ttattaaaaa aggtgattta
    14101 ctagttggga tggatggaga tttcaaccgt gagt
//
LOCUS       DAPGRN010000120        14012 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPGRN010000120 DAPGRN010000000
VERSION     DAPGRN010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.122"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(244..759)
                     /gene="pgpA"
                     /locus_tag="RHW33_003837"
     CDS             complement(244..759)
                     /gene="pgpA"
                     /locus_tag="RHW33_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV1647294.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGIL"
     gene            complement(737..1714)
                     /gene="thiL"
                     /locus_tag="RHW33_003838"
     CDS             complement(737..1714)
                     /gene="thiL"
                     /locus_tag="RHW33_003838"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV1647295.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVVPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(1792..2211)
                     /gene="nusB"
                     /locus_tag="RHW33_003839"
     CDS             complement(1792..2211)
                     /gene="nusB"
                     /locus_tag="RHW33_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV1647296.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(2231..2701)
                     /gene="ribE"
                     /locus_tag="RHW33_003840"
     CDS             complement(2231..2701)
                     /gene="ribE"
                     /locus_tag="RHW33_003840"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV1647297.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(2790..3893)
                     /gene="ribD"
                     /locus_tag="RHW33_003841"
     CDS             complement(2790..3893)
                     /gene="ribD"
                     /locus_tag="RHW33_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV1647298.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(3897..4346)
                     /gene="nrdR"
                     /locus_tag="RHW33_003842"
     CDS             complement(3897..4346)
                     /gene="nrdR"
                     /locus_tag="RHW33_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV1647299.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            4496..5035
                     /locus_tag="RHW33_003843"
     CDS             4496..5035
                     /locus_tag="RHW33_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV1647300.1"
                     /translation="MNINVFRLILLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            5334..6218
                     /gene="tsx"
                     /locus_tag="RHW33_003844"
     CDS             5334..6218
                     /gene="tsx"
                     /locus_tag="RHW33_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV1647301.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(6395..6742)
                     /locus_tag="RHW33_003845"
     CDS             complement(6395..6742)
                     /locus_tag="RHW33_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV1647302.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(6871..7842)
                     /gene="secF"
                     /locus_tag="RHW33_003846"
     CDS             complement(6871..7842)
                     /gene="secF"
                     /locus_tag="RHW33_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV1647303.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(7853..9700)
                     /gene="secD"
                     /locus_tag="RHW33_003847"
     CDS             complement(7853..9700)
                     /gene="secD"
                     /locus_tag="RHW33_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV1647304.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(9728..10060)
                     /gene="yajC"
                     /locus_tag="RHW33_003848"
     CDS             complement(9728..10060)
                     /gene="yajC"
                     /locus_tag="RHW33_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV1647305.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(10083..11210)
                     /gene="tgt"
                     /locus_tag="RHW33_003849"
     CDS             complement(10083..11210)
                     /gene="tgt"
                     /locus_tag="RHW33_003849"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV1647306.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPTDKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(11266..12336)
                     /gene="queA"
                     /locus_tag="RHW33_003850"
     CDS             complement(11266..12336)
                     /gene="queA"
                     /locus_tag="RHW33_003850"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV1647307.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPLAINERVGE"
     gene            12429..13010
                     /gene="acpH"
                     /locus_tag="RHW33_003851"
     CDS             12429..13010
                     /gene="acpH"
                     /locus_tag="RHW33_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV1647308.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYAALETRFWQFYPRMMAQASRKAL"
     gene            complement(13031..>14012)
                     /locus_tag="RHW33_003852"
     CDS             complement(13031..>14012)
                     /locus_tag="RHW33_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647309.1"
                     /translation="LKNHIKQLEEWALLEKFYDNNAYYSKRTKQLEKKITDAGFITGK
                     VNAEKFNVKEFKDDITSMLCEQPPLRTHQISSSGYVSADKLPKLYELNLKDAQRDLKT
                     TPVDIDGVSLKFKTRERIAEEIAKKTAEKTAKKNSEIIAKQNANSIEEKNIEKNNKET
                     NPEDNFVLKFSQEEINEMLLDYSDEEQKKLRPFFTSDHIYCLFDAFSQCLYNVARDGS
                     PRYAGLLQQIASKCAILQDFDQQLFWRTFSGGVFHRRISVNSEPEKTDVWRFSTQFDF
                     PHINDNGELSRTVLMFEGVAESNFKDIANHSSECFSYSCYAAPLDVNAKK"
BASE COUNT         3452 a         3739 c         3364 g         3457 t
ORIGIN      
        1 aattttgatg atatttttcg tcaacagtca gagaatatga gcaattatta tgagttcgtc
       61 gactgacgat atttcagtgt cattgaatta tgagtgacaa agatacacct tgaaatatcg
      121 ccgcatagta tattcggtga tgtcgggttt tactttaaca gagtgcccca aaattggggc
      181 actgttcatt tataaatcaa accgggtggt ctggaatcgc ctacacctcc cggtcaatgg
      241 catttaaaga atacccagcg gccagtgatg accgataaag tacaggatgc ctgcggagat
      301 caccccggcg acaatatcgt cgatcatgat ccccatgccg ccatgtacat tgcgatcaaa
      361 ccagcggatc ggccacggct tccacatatc cagaatacgg aaaatcacaa acccggcggc
      421 gacccactgc cagtcattgg tcggcagcgc catcagggtg atccacatgc caataaactc
      481 atcccagacg atgctaccgt gatcgtgcac acccatgtct ttcgccgttt gatgacaaag
      541 atagacgccg atacagatcc ccaacatcac caccagcgag tagagctgcc agggcaaaaa
      601 ggtcatcaga taccagaacg gaatcgccgc cagcgatccc atcgtgccag gaacgattgg
      661 gcttaatcca cttccgaatc caacagcaag cagatgccac ggattactca tcttcaggcg
      721 acttttcgcg acatctttat ggcgtggcaa aatggtcata tcctttccag tctaacgtga
      781 caggttcgcc gtcacgaata aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac
      841 aggtaaacgg tacgcccaga tgaccgagag ccacatccag cgcgccacgg ttcagttccg
      901 gcaccgtgaa acacaactcg taatcttcac cgccagagag cgcccagcgc agcgcctgtt
      961 ccggtacaac atggcgagaa agcgcatcag aaaacggcag caatgccagg tcaatacgtg
     1021 cgccgcagtc gctggctttc acgatatgcc cgagatcgga aatcagaccg tcagagagat
     1081 cgatagctga atttgccaga tcgcgcagtg cctgcccctg taaaatacgc ggcgatggac
     1141 ggagatgacg tttgatcaag tagtccgcat ctttagcatc ggcaacctgc aaacggtttt
     1201 gcaaaatcgc cagcccggcg gcgctatcgc ccggtgtacc ggtcacatag atccagtcac
     1261 ccggtttcgc cccagagcgc gttaaggctc gtcccatcgg aacaaagccg tggataccca
     1321 acgtcattga taatggccca cgcgtggtat cgccgccaat gagttgcata tcgtaataat
     1381 tgagaagatc aaacaaactg tcgctgaagg actcaagcca cgcttcgtct acgtccggta
     1441 aggttaatgc cagcgtcagc caggccggat cggcccccat cgctgccaga tcgcttaggt
     1501 tcaccgccag tgctttataa gccagatcag caggatcgat atcagggagg aaatggttgc
     1561 ccgccaccag cgtatcagtg ctgatcgcca gggtctgttt ctcggggata ttgagaagag
     1621 cgcaatcgtc gccgatgccc agttcgacat caagacgaga acttcttaca cggtcaaaat
     1681 aacgggcaat cagggagaac tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag
     1741 aaaaggccgg cgatcatgga acggtcttcc gtgaatctac cggcctggat attacttttt
     1801 gttagggcga atcacaggtg ctgctttatc gagtacgccg ttgacgaact tatggctgtc
     1861 ttctgcgccg aacgatttcg ccagttcgat cgcttcgtta atggccactt tgtatggcac
     1921 atcgctacgt ttagacagtt cgtacagcgc aatgcgcagt actgcttttt ctacctgtcc
     1981 cagttcttcc agcaggcggg acaggtatgg cttcatcagt ccgtcgaggt atgcggtatt
     2041 agtcgccacc ccggccagca gctcacggaa gtacaggacg tcaacgtctt ttacatcctg
     2101 ttcagccagg aactggtatt caacatcagc gatgtcgttc tgggacaact gccaggagta
     2161 gagcgcctgg acggcacact cacgagcgcg gcgacgagca gcaggtttca cggatttccc
     2221 cttactaatt tcaggccttg atggctttca atacattaat catttcaagc gcggtcagtg
     2281 cagcttctgc acctttgttg ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc
     2341 tttcagtggt cagaacccca aaagcaaccg gaatttcgct gtcctgggca acatgcgcca
     2401 ggccgttgct tgcaccacca gcgacatatt caaaatgggc agtgccacca cgaataaccg
     2461 tacccagcgc aatcaccgcg tcgtatttac cggttttagc cagtgcaccc gccgccagcg
     2521 gcagctcata ggcaccaggc acccaaacaa cggtaatgtt ttcatctttt acctgaccga
     2581 tacgtttcag tgcatcaatt gcaccttcca gcaggctgtc attgataaag ttgttgaaac
     2641 gcgcgatggt gatggcgacg cgagcgtccg gggtagcaac gttagcttca ataatgttca
     2701 ttttcttcct tcgggttcga gtatggcccc gcaggggggc ggattttagc ataatatttc
     2761 gtgcgctgct tccctttcga gccgggagat catgcaccca ctaaatgcag gcaaacatcc
     2821 gggcctacat gacgtatctc tttgaattta aattgggggg cgtcagctaa tttctcaagc
     2881 cctggcagtg tgcataatcc acgggcgtcg ctgcctaata gtttaggtgc gatatagaca
     2941 atcagctcat cgactaaacc cgcctgcagc aatgcgccag cgagcgttgg ccccgcttcc
     3001 acccagatgc tgttaatctg ctgtttaccc agttgcatca tcagtacaac cagatccaga
     3061 tgacctttat gctctggaat cagcaaggta cgcaccgttt ccggccactc acgagaatct
     3121 tcctgcgtac gcgcgaacca ggtttcgccg ggctgctgca caatgtgatg ttccggcgtc
     3181 acgcgatttt ggctatcaat cacaatacgt accggctgac ggagattttg ttgcggatag
     3241 agtgcctgag tttgttcatc cagttcagac caacgcaccg ttaaggcagg atcatccgcc
     3301 agcaccgtag cgctgctggt taaaatggca tgactttgtg cgcgcagtcg ttgtacgtcg
     3361 cgccgcgcct gaggcgaagt gatccactgg ctttcgccgc tcgccatcgc cgtgcggcca
     3421 tcaagcgatg cgccaagttt taactgaata taaggaaagc cggtgcgcat ccgcttgaga
     3481 aagcctttat tcaattgctc ggcttcactc atcatcagcc cgtggctgac gtcaatgcca
     3541 gcctgttgca aacggtaaag tccacgccca gcgacctgcg ggttcgggtc ctgcatcgca
     3601 gcgaccacgc gcgctacgcc agcggcgatg agtgcgtcac agcacggtgg cgtacgacca
     3661 tgatggctac agggttcgag tgtgacatag gcggtcgcac ctttggcttt ttcacccgcc
     3721 atacgcaacg cgtgtacttc ggcatgtggt tcacccgctc gttggtggta accttcaccg
     3781 acaatttcgc catctttgac aatgacgcac ccgacattcg ggttggggtg ggtggtaaaa
     3841 cgtcctcgtt gcgccagctt tagcgcccgc gccatgtaat actcgtcctg cacggcttag
     3901 tcctccaggc gcgcgatctc ttcgccaaat tctttgatat cttcgaaact gcggtagaca
     3961 gaggcaaaac ggatataggc gactttatcg agctttttca attgctccat caccagattg
     4021 ccaatcatct tgctcggcac ttcgcgctca ccggtggcgc gcagctgcga tttaatatga
     4081 ttgattgcca tttcgacgtc atcggaactc accggacgtt tttccagcgc ccgcagcatt
     4141 ccgctacgta atttttcttc attaaacggt tcacgcacgt cgttgctttt tacaacacgc
     4201 ggcataacca gctccgccac ttcaaaggtg gtgaaacgtt cattacacac cagacactgc
     4261 cgacggcggc gtacggatga accctcgccc acgagacgag agtcaattac cttagtgtcc
     4321 acggcgaaac agaatgggca atgcatacgg cgtcctgacc aggtggttaa cagaaatcta
     4381 ttttaccctg aactgacgat acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc
     4441 tttcgctgct tttaccctct accattaggc taattgcgac gagaagggac gacctatgaa
     4501 tattaacgtt tttcgactga tcctgctggg aagcctgttc agccttagcg cctgtgtgca
     4561 gcaaagcgaa gtgcgacaga tgaaacacag cgtcagcacg ctgaaccagg agatgacgca
     4621 actcaatcaa gaaacagtca aaatcactca gcaaaacagg ctgaatgcaa aatccagcag
     4681 tggggtttac cttctgcctg gagcgaaaac accggcaaga ctggaaagcc agatcggtac
     4741 tttacgtatg tcgctggtga atattacgcc tgatacagat ggcaccaccc tgacgctacg
     4801 tattcagggc gagtcgaatg atcccttgcc cgcattcagc ggaactgttg aatatgggca
     4861 aattcaggga acaatagaca actttcagga aatcaatgta cagaatcaat tgattaatgc
     4921 ccctgccagc gtcctcgccc ccagcgatgt tgatattccg ttacagttaa aggggatttc
     4981 tgtggatcag ttaggctttg tacgtatcca cgacattcaa cctgttatgc agtaaactta
     5041 cagcggggcg attttgtgcg tcccgcaaca tccttcccca tcattttgtt actccgctta
     5101 cataacccag attgatagta aaagtttgca acaagggcga aagtcagtac aatccccgcc
     5161 cgaatgtgtg taaacgtgaa cgcaatcgat tacgtaaatg atagaactgt gaaacgaaac
     5221 atatttttgt gagcaatgat ttttataata ggctcctctg tatacgaaat atttagaaac
     5281 gcaatttgcg cctttttcac tcccgcaagg gattttcaaa cagtggcata catatgaaaa
     5341 aaacattact ggcagccggt gcggtactgg cgctctcttc gtcttttact gtcaacgcag
     5401 ctgaaaacga caaaccgcag tatctttccg actggtggca ccagagcgtt aacgttgtcg
     5461 gaagctatca cacccgtttc ggaccgcaga tccgcaacga tacctacctt gagtacgaag
     5521 cattcgctaa aaaagactgg ttcgacttct atggttatgc ggatgcgccg gtattcttcg
     5581 gcggtaactc cgatgcaaaa ggtatctgga accacggttc tccgctgttt atggaaatcg
     5641 aaccacgttt ctccatcgac aagctgacca atactgacct tagcttcggt ccgttcaaag
     5701 agtggtactt cgcgaacaac tacatttacg acatgggtcg taataaagat ggtcgccaga
     5761 gcacctggta catgggtctg ggtaccgata tcgacactgg cctgccgatg agcctgtcca
     5821 tgaacgtcta tgcgaaatac cagtggcaga actatggcgc agcgaacgaa aacgagtggg
     5881 acggttaccg tttcaaaatt aaatactttg tgccgattac cgatctgtgg ggcggtcagc
     5941 tgagctacat cggcttcacc aacttcgact ggggttccga tttaggggat gacagcggta
     6001 acgcaatcaa cggtattaag acccgtacta ataactctat cgcttccagc catattctgg
     6061 ctctgaacta cgatcactgg cactactctg tcgtagctcg ttactggcac gacggtgggc
     6121 agtggaacga cgatgcagaa ctgaacttcg gcaacggcaa cttcaacgtt cgctctaccg
     6181 gctggggtgg ttacctggta gtaggttaca acttctgatt atgaaaatgc cgggatttat
     6241 tcccggcatt tcagattgtt aacaacgtgc gctttgttca tgccggatgc ggcgtaaacg
     6301 ctttgtccgg cccacaaaag cacgtaaatt caatatattg ctgaaataac gtaggcctga
     6361 taagcgtagc gcatcaggca attttacgtt ttaatcactt cttcttgttc atcatcgctt
     6421 tcaggtcagc gaatgggttg tacttcgctt caccgacatc tttctgcgca tcttcccctg
     6481 cgataacggt cgtaccatac tgatccgctt cggtatattt cgaatgctca tgatcgtggc
     6541 aatagagaca aagcaattcc cagttactgc catcttccgg gttattggta tggtcgtgat
     6601 caatgtggtg aacggtaagt tcacgcaggt tggaataaac aaactcgcgg gaacagcgac
     6661 cgcagaccca cggatagatt tttaatgctt tttcgcgata gccgctttct aaccgcgcat
     6721 agttttttgg gatgatagcc atgaaaagtt acctggatga aatgtagggt tacgcgcagt
     6781 ttcccaagat gcggggattt tcgcaatctc catacatcaa aaaaaatccc gatcttctga
     6841 ccgggatttt caacatcaac gggaacttga ttacggcaga attgacggct gatccgcccc
     6901 ttctttttcc actttctgct gcaacatgtg ttcgcgcttc atacccagtt tcagagccaa
     6961 cgcagatgcc acatagatgg aagatgcagt accgatggaa acaccaatca gcatggtcag
     7021 cgagaagcct tccagtaccg gaccaccgaa gaggtacagc atcaggataa ccatcaaggt
     7081 agtaccggat gtgatcaagg tacgatgcag cgtctgggtc aaggacacgt taaagatttc
     7141 gtaaggcgta ccgcgacgga tcttgcggaa gttttcacga attcggtccg ataccacgat
     7201 actgtcgtta agcgagtaac cgataaccga catcaacgat gccacaatgg tcaggtcaat
     7261 ctcgatatgg aataacgaca aaatacccag cgtaatgata acgtcgtgcg ccagcgcaat
     7321 aaccacccct gccgccagtc gccactcaaa gcggaaacct acgtacacga ggatagacag
     7381 cagcgctgcc atcagcgcca tcgcaccggt ttgcgcaagg tctgccccca cgctcggacc
     7441 gacgaactca atacgcttca ctgctgcatt ctgattggtg gattcgttaa tcaccttcag
     7501 aacctggctg cccagcacct gaccgccggt ttcgccttca gcaggcggca tacggaccat
     7561 gatgtcgtgg ctgctaccaa agttttgcag catcggctct tcaaaaccgg ctttttgcaa
     7621 tgcatcacgc attacgtcaa tttcagccgg tttttcgagc gtaatttcga taaccgtacc
     7681 accggtgaaa tccagccccc agttaaagcc gcgcacaccc ataataacga tagcggcgat
     7741 taacagcaga ccagagatgc cgaaagccca gtagtcccag cgcataaagt catagacttt
     7801 acggccatgg tttagttgtt caacagtata ttcctgtgcc acatcgcact cctcagattg
     7861 acagcttctt gacgcgcttg ccgccatata gcaggtttac gatggcacgc gtaccgacaa
     7921 tcgcggtaaa catcgacgtc gccacaccga taccggtagt aatcgcgaac cctttaattg
     7981 ccccggtacc cactgcgtac aggatgatga ctttaatcag cgtggtgatg ttcgcatcga
     8041 agatagaact gaatgcgcca cgataacctt catcaattgc ctgttgaaca gtacgtccgt
     8101 tgctcaactc ttctttaata cgttcgttga tcagtacgtt cgcatcgacc gctaccgcaa
     8161 gggttaagac gatacccgcg atacctggca tactcagtgt tgcgcctggc aacagcgaca
     8221 taataccgac gatcaagatc aagttggcaa tcagagcact ggtcgcaatc agaccaaact
     8281 tcttatagaa gatgatcatg aacagaatag aaaccagcag accggcaagg caagcctcca
     8341 gcccctgctc aatattctgc atacccaggg tcgggccaat tgtacgttct tcaacaatct
     8401 gaatcggcgc gatcaacgca ccggcacgca gcagcagtga cagctgacgg gcttcgttcg
     8461 ggttgttgat gccggtgata cggaagctgt tacccagacg agactggatg ttggcgatgt
     8521 taatcacctc ttcctgtttc accagaaccg cacgaccatt tgcatctttc ttcccgctgt
     8581 ctttgtactc cacaaacagg gttgccatcg gtttgccgat gttgtccttg gtgaagttag
     8641 acataatgtt gccaccagcg ctatcgagcg agatgttaac ctgcggctgg ttgtattcgt
     8701 cctggctgga agtggagtcg gtgatatggt caccggtcag aattacgcgt ttgtacagca
     8761 caaccggctg accttcgcgg gtctgtttca cttcagagtc gcccggtacg cgaccggatg
     8821 ccgcagcggc ctgatcaacg ttggtgttta ccagacggaa ttccagcgtt gccgtcgcac
     8881 ccagaatctc tttcgcacgc gcagtgtcct gaatacctgg cagttcaaca acgatacggt
     8941 cagcaccctg acgctgaacc accggctccg ccacgccaag ttggtttaca cggttacgca
     9001 ggatattgat gttctgctgc accgcatatt cacgcgcttc actcagacga gcatcgctca
     9061 ttaccgcacg cagctggttg ctgccctggc tgctaatcac caggtccgga tggcgcttgc
     9121 tcagatacgc aatggcttca tcacgagctt tagcatcgcg gaaagtgatg ctcaggccgt
     9181 agttgttttc tttacgaacg gtggtatacg ggatgccttt ttcgcgcagg tcgctgcgca
     9241 ggctatcgat attttgttcc tggagtttgc caagcgcggt gtccatatcc acttccatca
     9301 ggaagtgaac gccgccacgc aggtcaaggc cgagcttcat cggctcagcg tgaatagctg
     9361 ccagccagcg cggcgttgcc ggggcaaggt taagcgccac gacgtacttg tcacccataa
     9421 cgcccattaa tgcttcacga gcgcgcagct gggtgtcagt ggagtcgaag cgcgcaagaa
     9481 tagcgccctc ttccagtgcc acagacttag cagttatttt ttcttcttgt aacgttttct
     9541 ggacctggat cagcgtttgc tcactggcgg cgactccgcg cgcaccagtg atctgaacag
     9601 ccggatcctc accaaacagg ttgggaagcg catacagcag accgatgaca atcaccacga
     9661 tcagcatgac gtacttccac aaaggataac ggtttaacac ggcaattccc ttagggaaaa
     9721 attttaatta cagcgccttc atggtgcctt tcggcaggac ggcagctacg aagtcacgtt
     9781 taataactac ttcagtggtg tcattcagcg cgatagcaat gtagccgttt tccgctactt
     9841 tggttacgcg accgaccagg ccaccgttcg tcagaacttc atcacctttg gcaatggagt
     9901 ccatcagctt tttgtgttct ttggtgcgct tctgctgtgg acgcaggatc atgaaataga
     9961 aaatcagacc gaataccacc agcatcaaaa tcaaagacat cgggctacct tgcgccggtg
    10021 cacccgttgc cgctaccgca tcagaaataa aaaagctcat taaatttccc tcattattaa
    10081 tattaatcaa cgttcaaagg tggtacttct cgcccctgac gctggtaaaa atcagttacg
    10141 aagctctcta atttaccctc ttcaatagcc ttgcgtaaac ccgccatcaa acgctggtag
    10201 taacgaaggt tatgaatggt gttgagtcgc gcgcctaata tttcgttgca acggtcaaga
    10261 tgatgcaagt aagcgcgtga ataattgcga caggtgtagc aatcacactc aggatcgagt
    10321 gggccagtat cgctcttata cttcgcattg cggattttca ccacgccatc ggtcacgaac
    10381 aaatgaccat ttcgggcgtt gcgggttggc attacgcagt caaacatatc gataccacga
    10441 cgtacgcctt caaccaggtc ttctggttta ccaacgccca tcaggtaacg cggtttgtct
    10501 gtcggaattt gcgggcatac atgctccaga atgcggtgca tatctgcttt cggctcaccc
    10561 acagccagac cgccgacagc gtagccatca aaaccgatat ctaccagacc tttaacagaa
    10621 atatcacgta aatcttcgta aacgctgccc tgaatgatac caaacagcgc atttttgttt
    10681 ccgagactgt caaaacgctc acggctacgc ttcgcccaac gcagagacat ctccatggag
    10741 cgttttgcgt aatcccagtc agcaggatac ggcgtacact catcaaagat catgacgata
    10801 tccgaaccaa gatcgtactg aatctccatc gacttttccg ggtcgaggaa aatcgggtcg
    10861 ccgttgatcg ggttacggaa gtgcacgccc tgttcggtga ttttacgaat atcgccaagg
    10921 ctgaagacct ggaagccgcc ggagtcggtg aggatcggtc ccttccactg cataaaatcg
    10981 tgcagatcgc cgtgcagttt catgatttcc tggcccgggc gcagccacag gtggaaggtg
    11041 ttgccgagga taatttgcgc gccagtggct tcaacttctt ctggcgtcat cccttttacg
    11101 gtgccgtagg tgccaacagg cataaaacaa ggcgtttcca ctacgccacg atcaaagacc
    11161 aggcggccac ggcgtgcgcg accgtcggtg gtgtccagtt caaatttcat tttttctcca
    11221 ctacgtcaga aaaacagtcc aacgttttaa accagcgccg cggaattact ccccgacgcg
    11281 ctcattaatt gccagcggat tgtacgtgat aaacatcgca tcaccgtaac taaaaaagcg
    11341 atatttctct tctaccgctg ctttataggc gttcatggtg tgttgataac cggcaaaggc
    11401 cgaaaccagc ataatcagcg tcgactctgg caagtggaag tttgtcacca gcgcatcgac
    11461 cactttgtac tggaagcccg gatagataaa gatttgggta tcgtcaaaga acggttcgat
    11521 gagatcgttt ttcgccgcct gagccgcgct ttccagcgaa cgtactgagg tggtgccaac
    11581 cgcaatcacc cggttaccgc gcgctttcgc cgccagtacc gcgtctacca catcctgcgg
    11641 tacttcagcg tattccgagt gcatgatgtg atcttcaatg gtgtcgacgc gcaccggctg
    11701 gaaggtgccc gcaccaacgt gcagggtgac gaacgccatc tccacgcctt tggcgcgtaa
    11761 tttttccagc aaaggttcgt caaaatgcag accagcggtc ggggctgcaa ccgcgcccgg
    11821 tttttcgcta taaacggttt gataaagttc gcggtcagcg tcttcgtccg gacggtcgat
    11881 atacggcggc agcggcatat ggccgatgct gttgagaata tccagtaccg agcgttcatc
    11941 attaaattcg acttcaaaca gtgcgccgtg gcgcgcggtc attgttgcgt taatactttc
    12001 gtcatcgccc agcagcagtt ctgcgccagg ttttggcgct ttcgaggcgc gaatatgcgc
    12061 aagaatgcgt ttgtcgtcga gcatccgctc aaccagcact tcaatcttgc cgccactggc
    12121 tttacgccca aacagacgcg ccgggatcac ccgggtatta ttaaaaacca gaagatcgcc
    12181 ggggttgagc ttatcgagta aatcggtgaa agtaccgtgc gtcagcgcgc ccgtcggccc
    12241 gtccagcgac agtaaacgac agctactgcg ttcaggcatc ggatagtggg caatcaggga
    12301 ttcgggcaat tcaaaggaga aatcggtaac gcgcatgaca ctgactcaga ctaaaataag
    12361 aggcgggtag tctagtgccg gggcgctctc cctgcaacct ttacccccct ttggatagaa
    12421 tataatcgat gaatttttta gctcacctgc atttagccca tctcgcggaa agctcgcttt
    12481 ccggcaattt gctggctgat ttcgtacgcg gaaatcctga agaaagtttt ccgcccgacg
    12541 tcgtggctgg cattcatatg catcgacgta tcgacgtatt gactgacaat ctgccggaag
    12601 tccgcgaagc acgggaatgg tttcgtagtg aaacgcgccg cgttgcgcct attacgctgg
    12661 atgtcatgtg ggatcacttt ctttcccgcc actggtcgca gctgtcgccg gactttccgc
    12721 tacaggaatt tgtctgttat gcccgcgagc aagtgatgac gattttgccg gactcaccgc
    12781 cacgttttat caatctgaac aattacttgt ggtcagagca gtggctggtg cgctatcgcg
    12841 atatggattt catccagaac gtgttaaacg gcatggcaag ccgtcgccca cgtctggatg
    12901 ccctgcgtga ctcctggtac gatttaaacg cacattatgc cgccctcgaa acccgcttct
    12961 ggcagtttta cccgcggatg atggcgcagg cgtcacgcaa ggcgctctga aacttcaagt
    13021 tccggcgtca ctatttttta gcattcacat ccagcggagc ggcataacaa ctgtaagaaa
    13081 aacattcgct ggaatgattg gcgatatctt taaagttgct ttcagcaacc ccctcgaaca
    13141 tgagtaccgt tctggacaac tctccgttgt catttatatg tggaaaatca aattgtgtac
    13201 tgaatcgcca tacatctgtc ttttctggct cgctgttcac agagatacgt cgatggaaga
    13261 ctcctcctga aaacgtgcgc cagaacaatt gttgatcaaa atcttgtaat atcgcacact
    13321 tggatgctat ttgttgtagt aaacctgcat accgtggact gccatcacga gcaacattat
    13381 acaaacactg actaaaagca tcgaacaaac aatagatgtg atcactagtg aaaaaaggtc
    13441 ttaatttttt ttgttcttcg tcgctgtaat ccaggagcat ctcattgatt tcttcctgtg
    13501 agaattttaa gacaaaatta tcttcaggat ttgtttcttt gttgtttttt tcaatattct
    13561 tctcctcaat gctattcgca ttttgtttcg caattatttc cgaatttttt ttcgcagttt
    13621 tttccgcagt ttttttcgca atttcttctg caattctttc ccgagtttta aattttaaac
    13681 tgacaccatc gatgtcaacc ggtgttgttt taagatctct ctgcgcgtct ttcaaattta
    13741 actcatagag tttgggcagt ttatctgcag atacgtatcc ggatgaagaa atctggtgtg
    13801 tacgcagggg cggctgttca caaagcatcg aggttatatc gtctttaaac tcttttacat
    13861 taaacttctc ggcattaacc ttccctgtta taaaccccgc atcggtaatt ttcttctcta
    13921 attgttttgt acgtttagaa taataagcat tgttatcata aaacttttca agaagagccc
    13981 actcctcaag ttgctttatg tgatttttca ac
//
LOCUS       DAPGRN010000121        13902 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPGRN010000121 DAPGRN010000000
VERSION     DAPGRN010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.123"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..461
                     /locus_tag="RHW33_003853"
     CDS             102..461
                     /locus_tag="RHW33_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV1647310.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(581..760)
                     /locus_tag="RHW33_003854"
     CDS             complement(581..760)
                     /locus_tag="RHW33_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV1647311.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            834..2195
                     /gene="pabB"
                     /locus_tag="RHW33_003855"
     CDS             834..2195
                     /gene="pabB"
                     /locus_tag="RHW33_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV1647312.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGEILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2199..2777
                     /locus_tag="RHW33_003856"
     CDS             2199..2777
                     /locus_tag="RHW33_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV1647313.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2961..4325
                     /gene="sdaA"
                     /locus_tag="RHW33_003857"
     CDS             2961..4325
                     /gene="sdaA"
                     /locus_tag="RHW33_003857"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV1647314.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGQ
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4456..6054
                     /locus_tag="RHW33_003858"
     CDS             4456..6054
                     /locus_tag="RHW33_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1647315.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDNRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6058..7614)
                     /gene="yoaE"
                     /locus_tag="RHW33_003859"
     CDS             complement(6058..7614)
                     /gene="yoaE"
                     /locus_tag="RHW33_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV1647316.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            8077..9048
                     /gene="manX"
                     /locus_tag="RHW33_003860"
     CDS             8077..9048
                     /gene="manX"
                     /locus_tag="RHW33_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV1647317.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9111..9911
                     /gene="manY"
                     /locus_tag="RHW33_003861"
     CDS             9111..9911
                     /gene="manY"
                     /locus_tag="RHW33_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV1647318.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9924..10775
                     /gene="manZ"
                     /locus_tag="RHW33_003862"
     CDS             9924..10775
                     /gene="manZ"
                     /locus_tag="RHW33_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV1647319.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10830..11288
                     /locus_tag="RHW33_003863"
     CDS             10830..11288
                     /locus_tag="RHW33_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV1647320.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11718..12284
                     /gene="mntP"
                     /locus_tag="RHW33_003864"
     CDS             11718..12284
                     /gene="mntP"
                     /locus_tag="RHW33_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV1647321.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12281..13090)
                     /gene="rlmA"
                     /locus_tag="RHW33_003865"
     CDS             complement(12281..13090)
                     /gene="rlmA"
                     /locus_tag="RHW33_003865"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV1647322.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13256..13465)
                     /gene="cspE"
                     /locus_tag="RHW33_003866"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13256..13465)
                     /gene="cspE"
                     /locus_tag="RHW33_003866"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV1647323.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13478..13621)
                     /locus_tag="RHW33_003867"
     CDS             complement(13478..13621)
                     /locus_tag="RHW33_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV1647324.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3430 a         3479 c         3463 g         3530 t
ORIGIN      
        1 ctccatctta ctggataaca caccatgagc tgctaccttt aaaacacctg aatgtcaaca
       61 ggttaactcg tgccatatcg ttataatccc tttgatatta gatgcaaatt aaggtcatat
      121 atagccttat cgataacatg gttaatttta aggataagaa tatgcctgct gtaatagata
      181 aagccctgga tttcattggt gccatggatg tatcagcgcc aacaccaagt tcgatgaatg
      241 aaagcacggc gaagggaatc tttaaatatt taaaagaact gggagtaccc gcgagtgccg
      301 ctgatattac ggcgcgagct gaccaggaag gctggaaccc tgggttcacg gaaaaaatgg
      361 ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt gattaaaaat cctgagtact
      421 tttcaacata tatgcaggaa gaactaaaag cactggtctg agttaaattt atatcagcat
      481 aaatgggtca ggacgctttt aatcacatat aaaaagcgtc ctttttctca caatcaaata
      541 atcatcggct tctataacga aatcgatacg gattagtgtg ttactcgtct tcatcctcaa
      601 aacgcgccac gattcgctcg ccggagtggt tggcacgcag ctcttctgcc accagcgcga
      661 ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg gatccgctcg acagcttttt
      721 gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc tgctaaattg
      781 ttggcgctaa ttatttcatg ctacccggca catagccagt agagtcagga ctgatgaaga
      841 cgttatctcc cgctgtgatt actttaccct ggcgtcagga cgccgctgaa ttttatttct
      901 cccgcttaag ccacctgccg tgggcgatgc ttttacactc cggctatgcc gatcatccgt
      961 atagccgctt tgatattgtg gtcgccgatc cgatttgcac tttaaccact ttcggtaaag
     1021 aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac tgatgacccg ctacaggtgc
     1081 tccagcaggt gctggatcgc gcagacattc gcccaacgca taacgaagat ttgccatttc
     1141 agggcggcgc actggggttg tttggctacg atctgggccg ccgttttgag tcactgccag
     1201 aaattgcgga acaagatatc gttctgccgg atatggcagt gggtatctac gattgggcgc
     1261 tcattgtcga ccaccagcgt catacagttt ctttgctaag tcataatgat gtcaatgccc
     1321 gtcgggcctg gctggaaagc cagcaattct cgccgcagga agatttcacg ctcacttccg
     1381 actggcaatc caatatgacc cgcgagcagt acggcgaaaa atttcgccag gtacaggaat
     1441 atctgcacag cggtgattgc tatcaggtga atctcgccca gcgttttcat gcgacctatt
     1501 ctggcgatga atggcaggca ttccttcagc ttaatcaggc caaccgcgcg ccatttagcg
     1561 cttttttacg tcttgaacag ggtgaaattt taagcctttc gccagagcgg tttatccttt
     1621 gtgataatag tgaaatccag acccgaccga ttaaaggcac gctaccacgc ctgcccgatc
     1681 ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc agcgaaagat cgtgccgaaa
     1741 atctgatgat tgtcgattta atgcgtaatg atatcggtcg tgttgccgta gcaggttcgg
     1801 taaaagtacc agagctgttc gtggtggaac ccttccctgc cgtgcatcat ctggtcagca
     1861 ccataacggc gcgactacca gaacagttac acgccagcga tctgctgcgc gcagcttttc
     1921 ctggtggctc aataaccggg gctccgaaag tacgggctat ggaaattatc gacgaactgg
     1981 aaccgcagcg acgtaatgcc tggtgcggca gcattggcta tttgagcttt tgcggcaaca
     2041 tggataccag cattactatc cgcacgctga ctgccattaa cggacaaata tactgctctg
     2101 cgggcggtgg aattgtcgcc gatagccagg aagaagcgga atatcaggaa acttttgata
     2161 aagttaataa gatattacgc caactggaga agtaagacgt ggaataccgt agcctgacgc
     2221 ttgatgattt tttatcgcgc tttcaacttt tgcgcccgca aattaaccgg gaaaccctaa
     2281 atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg accgcaaccg gggttgttgc
     2341 tgactcagcg ttcaattcat ctgcgtaaac acgctggaca agtggcattc cctggaggtg
     2401 cagtcgatga cacggacgca tcagttatcg ccgccgcgct gcgcgaagct gaagaagagg
     2461 tcgctatacc gccttccgcc gttgaagtta tcggcgtgct gccgcccgtc gatagcgtaa
     2521 ctggctacca ggtaacccca gtggtcggca ttatcccgcc cgatctgccg tatcgcgcca
     2581 gtgaagatga agtctcggcg gtgtttgaaa tgccgctcgc ccaggcatta catctgggtc
     2641 gttatcaccc tttagatatc taccgccgtg gcgattcaca tcgggtatgg ctgtcctggt
     2701 acgaacagta ttttgtatgg ggaatgaccg caggcataat tcgtgagctg gcgctgcaaa
     2761 ttggtgtgaa accctgacta tacttatctt tacatctaca aaacactact tgagacaatc
     2821 atcgcaatat tagttaaatc gcggtttttg attagtttaa ttcatgtgaa tagttaagcc
     2881 agtcgccgcg ttccctctta cactatgcgc tgttattagt tcgttactgg aagtccagtc
     2941 accttgtcag gagtattatc gtgattagtc tattcgacat gtttaaggtg gggattggtc
     3001 cctcatcttc ccataccgta gggcctatga aggcaggtaa acagttcgtc gatgatctgg
     3061 tcgaaaaagg cttactggat agcgttactc gcgttgccgt ggacgtttat ggttcactgt
     3121 cgctgacggg taaaggccac cacaccgata tcgccattat tatgggtctt gcaggtaacg
     3181 aacctgccac cgtggatatc gacagtattc ccggttttat tcgcgacgta gaagagcgcg
     3241 aacgtctgct gctggcacag ggacagcatg aagtggattt cccgcgcgac aacgggatgc
     3301 gttttcataa cggcaacctg ccgctgcatg aaaacggtat gcaaatccac gcctataacg
     3361 gcgatgaagt cgtctacagc aaaacttatt attccatcgg cggcggtttt atcgtcgatg
     3421 aagaacactt tggtcaggat gctgccaacg aagtgagcgt gccgtatccg ttcaaatctg
     3481 ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc
     3541 agaacgaact ggcgctgcac agcaagaaag agatcgacga gtatttcgcg catgtctggc
     3601 aaaccatgca ggcatgtatc gatcgcggga tgaacaccga aggtgtactg ccaggcccgc
     3661 tgcgcgtgcc gcgtcgtgcg tctgccctgc gccggatgct ggtttccagc gataaactgt
     3721 ctaacgatcc gatgaatgtc attgactggg taaacatgtt tgcgctggca gttaacgaag
     3781 aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc
     3841 cggcagtgct ggcttactat gaccacttta ttgaatcggt cagcccggac atctataccc
     3901 gttactttat ggcagcgggc gcgattggtg cattgtataa aatgaacgcc tctatttccg
     3961 gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc
     4021 ttgcagaact gctgggcggt agcccggaac aggtttgcgt ggcggcggaa attggcatgg
     4081 aacacaacct tggtttaacc tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg
     4141 agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc acggatggct ctgcgccgca
     4201 ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa accggtaagg
     4261 acatgaacgc caaataccgc gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg
     4321 actaatactt cttactcgcc catctgcaac ggatgggcga atttatcccc gctttctcgt
     4381 ctgctgtaat attccccact acacttccac tgttgcgtca ggcgtttgtc gccatacgct
     4441 tacagggtgg cccgcatgca aaaagcacaa cggatcatta aaacctatcg ccgtaatcga
     4501 atgattgttt gtacgatttg cgcactcgtt acgctcgctt cgaccctgag cgtgcgattt
     4561 atttcacagc gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca cgctgtagag
     4621 gaattagata aagtactgct tcccctacag gcaggtagcg aagtcttgct tccgctgatt
     4681 ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa actccaaact
     4741 gtgcgatcca ttggcctggt gcaagacggc acactttatt gctccagcat ttttggttat
     4801 cgcaatgtgc ccgtcgtgga cattcttgct gaacttcctg caccgcaacc acttttacgc
     4861 ctgacgatcg accgtgccct gattaaaggc agtccggttt tgattcaatg gacgcctgca
     4921 gcgggcagta gcaatgctgg ggtcatggag atgattaaca tcgacttact gacggcaatg
     4981 ctgcttgagc cacaactgcc gcaaatcagt agcgccagcc tgacggtgga caatcggcat
     5041 ttgctctatg gtaatgggct ggtagattcc cttccgcaac ctgaagacaa tgaaaactat
     5101 caggtttctt cgcaacgctt tccttttacc attaacgtta atggtccggg ggctacggcg
     5161 ctggcatggc actatcttcc aacacaatta ccgctggcgg tgctgctaag tttactggtg
     5221 ggctacatcg cctggctggc gaccgcttac cggatgagct tttcccgcga aatcaatctg
     5281 ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc gcgcagccag
     5341 caatgtattg gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg ctggatttca
     5401 ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact gacccgctat
     5461 gtgatggcag aaaccattcg tcagcgccat gttttcccga tgagtagtca gtttcatgtt
     5521 ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga tctcaatcag
     5581 tactggttta gcgctcaccc gattcagcaa ctgatcctcg aaatcaccga acgcgatgcc
     5641 ttactggatg ttgattatcg gattgcccgc gagctgcatc gtaaaaacgt caaactggcg
     5701 attgatgact tcggcaccgg caacagctcg ttttcctggc ttgaaacatt acgtcctgac
     5761 gtgctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt taactcgacg
     5821 gtgaccgata tcatcatcgc actggggcaa agactgaata ttgaactggt ggcggagggc
     5881 gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg gcgtgcatat tttgcaaggg
     5941 tatttgtacg cacagccgat gccgctacgt gattttccca aatggctggc gggcagccaa
     6001 ccgccgcccg cccggcataa tggacatatc acgcccatta tgccgttacg ttaacgctta
     6061 ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga accagatcaa cacgataatc
     6121 attggcttca atgatggtga tatgcagtgg ccctacatca atcacatcgc ccacacgggg
     6181 aatgtgacca tttgccgaga tcacgaggcc cgcgaccgtc gcgatatcat cgtcatcggc
     6241 aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc cttttaccag
     6301 ccagccgtca ccatcagtaa tgatttccgg cgtttcgtca gcgtccggga attcacccgc
     6361 aatggcttcc agcacatcca gcggcgtgac cagaccttgt accacaccaa actcgttggt
     6421 cacgataaca aagctcccgc gagcacgacg cagcacgccc agcaggttga tcggatcgag
     6481 ggtttccggg acgataatcg ccggagacgc cgaagcaatc gccgccacat caacgccctc
     6541 ttccagcgcc accagcagtt ctttagcacg tacgattccg atgatttcat ccagttcacc
     6601 gcgacatacc gggaacaggc tgtgcggtga ggagagcagc tgctcgcgga tttcatcgac
     6661 cccgagatta gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc cgcgcagaga
     6721 acgcgacgcc agcgtcagta cgccgttaat catgtaacgt tcttcttcgg caaatgcacc
     6781 ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg cctgacgttt
     6841 cccgcccatc aaacgcagga tagcatcggc agtacgcgct cgcagcggca aagtcgactg
     6901 gtgacgaata aagttgcgac gcgcaatctg gttaaacact tcgatgatga tcgagaagcc
     6961 aatcgcggca tacaggtaac ctttcggaat gtggaaaccg aaaccttctg ccaccagact
     7021 cagaccaatc attaacagga agctcagaca gagcaccacc accgtggggt gctggttaac
     7081 gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc atcgcaatca ctaccgccgc
     7141 catcatcacc ggcagatggt taaccatccc tactgcagta attaccgcat ccaacgagaa
     7201 gacggcgtca aggatgacaa tctgtgtgac gaccacccag aaactggcgt agcctttacc
     7261 gtggccggaa tcatgatcgc ggttttccag ccgttcatgc agttcggttg ttgctttgaa
     7321 cagcaagaat atccccccga acaacataat caggtcgcgt ccggagaagg agaaatccat
     7381 gacggtaaat agcggtttgg tcagcgtgac catccatgaa atcagcgaca gcagccccag
     7441 acgcataatc agcgccagtg ataaccccag caaacgcgct ttatcgcgtt gttttggcgg
     7501 cagtttgtca gcaagaatgg cgatgaagac caggttatcg atacccagca caatttcgag
     7561 aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt ccatgacaag
     7621 ctcctgctta aggaatagct attcgacgcc agaaataatg caggcgtaac gacaaaatgc
     7681 aaacgaaagg tgcggcatag agtgccagaa aggcaggcgt taaaaggcct gatgctgaaa
     7741 tgacgtcggt gacgatccat actgcgggct actgccctat actccatgat tgttaaacgg
     7801 gagttaaaca tatcagagac gcctctgatt tggcaaagat ttaccttcct ttgcaaacga
     7861 atgtgacaag gatattttac ctttcgaaat ttctgctaat cgaaagttaa attacggatc
     7921 ttcatcacat aaaataattt tttcgatatc taaaataaat cgcgaaacgc aggggttttt
     7981 ggttgtagcc cttatctgaa tcgattcgat tgtggacgac gattcaaaaa tacatctggc
     8041 acgttgaggt gttaacgata ataaaggagg tagcaagtga ccattgctat tgttataggc
     8101 acacatggtt gggctgcaga gcagttgctt aaaacggcag aaatgctgtt aggcgagcag
     8161 gaaaacgtcg gctggatcga tttcgttcca ggtgaaaatg ccgaaacgct gattgaaaag
     8221 tacaacgctc agttggcaaa actcgacacc actaaaggcg tgctgtttct cgttgataca
     8281 tggggaggca gcccgttcaa tgctgccagc cgcattgtcg tcgacaaaga gcattatgaa
     8341 gtcattgcag gcgttaacat tccaatgctc gtggaaacgt taatggcccg tgatgatgac
     8401 ccaagctttg atgaactggt tgcgctggca gtagaaacag gccgtgaagg cgtgaaagca
     8461 ctgaaagcca aaccggttga aaaagccgcg ccagcacccg ctgccgcagc accaaaagcg
     8521 gctccaactc cggcaaaacc aatggggcca aacgactaca tggttattgg ccttgcgcgt
     8581 atcgacgacc gtctgattca cggtcaggtc gccacccgct ggaccaaaga aaccaatgtc
     8641 tcccgtatta ttgttgttag tgatgaagtg gctgcggata ccgttcgtaa gacactgctc
     8701 acccaggttg cacctccggg cgtaacagca cacgtagttg atgttgccaa aatgattcgc
     8761 gtctacaaca acccgaaata tgctggcgaa cgtgtaatgc tgttatttac caacccaaca
     8821 gatgtagagc gtctcgttga aggcggcgtg aaaatcacct ctgttaacgt cggtggtatg
     8881 gcattccgtc agggtaaaac ccaggtgaat aacgcggttt cggttgatga aaaagatatc
     8941 gaggcgttca agaaactgaa tgcgcgcggt attgagctgg aagtccgtaa ggtttccacc
     9001 gatccgaaac tgaaaatgat ggatctgatc agcaaaatcg ataagtaacg tattgtgttg
     9061 attatcactc agttttcaca ctttagtctt acgtaaacag gagaagtaca atggagatta
     9121 ccactcttca aattgtgctg gtatttatcg tagcctgtat cgcaggtatg ggatcaatcc
     9181 tcgatgaatt tcagtttcac cgtccgctaa tcgcgtgtac cctggtgggt atcgttcttg
     9241 gggatatgaa aaccggtatt attatcggtg gtacgctgga aatgatcgcg ctgggctgga
     9301 tgaacatcgg tgctgcagtt gcgcctgacg ccgctctggc ttctatcatt tctaccattc
     9361 tggttatcgc aggtcatcag agcattggtg caggtatcgc actggcaatc cctctggccg
     9421 ctgcgggcca ggtactgacc atcatcgttc gtactattac cgttgctttc cagcacgctg
     9481 cggataaggc tgctgataac ggcaacctga cagcgatttc ctggatccac gtttcttcac
     9541 tgttcctgca agcaatgcgt gtggctattc cggccgtcat cgttgcgctg tctgttggta
     9601 ccagcgaagt acagaacatg ctgaatgcga ttccggaagt ggtgaccaat ggtctgaata
     9661 tcgccggtgg catgatcgtg gtggttggtt atgcgatggt tatcaacatg atgcgtgctg
     9721 gctacctgat gccgttcttc tacctcggct tcgtaaccgc agcattcacc aactttaacc
     9781 tggttgctct gggtgtgatc ggtactgtta tggcagtgct ctacatccaa cttagcccga
     9841 aatacaaccg cgtagccggt gcgcctgctc aggctgctgg taacaacgat ctcgataacg
     9901 aactggacta acaggtgagc gaaatggttg atacaactca aactaccacc gagaaaaaac
     9961 tcactcaaag tgatattcgt ggcgtcttcc tgcgttctaa cctcttccag ggttcatgga
    10021 acttcgaacg tatgcaggca ctgggtttct gcttctctat ggtaccggca attcgtcgcc
    10081 tctaccctga gaacaacgaa gctcgtaaac aagctattcg ccgtcacctg gagttcttta
    10141 acacccagcc gttcgtggct gcgccgattc tcggcgtaac cctggcgctg gaagaacagc
    10201 gtgctaatgg cgcagagatc gatgacggtg ctatcaacgg tatcaaagtc ggtttgatgg
    10261 ggccgctggc tggtgtaggc gacccgatct tctggggaac cgtacgtccg gtatttgcag
    10321 cactgggtgc cggtatcgcg atgagtggca gcctgttagg tccgctgctg ttcttcatcc
    10381 tgtttaacct ggtgcgtctg gcaacccgtt actacggcgt agcgtatggt tactccaaag
    10441 gtatcgatat cgttaaagat atgggtggtg gcttcctgca aaaactgacg gaaggggcgt
    10501 ctatcctcgg cctgtttgtc atgggggcat tggttaacaa gtggacacat gtcaacatcc
    10561 cgctggtggt ttcgaccatc accgggcagg atggacagac ccgtgtcacc accgtgcaaa
    10621 ctattcttga ccagttaatg ccaggccttg taccactgct gctgaccttt gcttgtatgt
    10681 ggctgctgcg caaaaaagtt aatccgctgt ggatcatcgt tggcttcttc gtcatcggta
    10741 tcgctggtta cgcttgcggc ctgctgggac tgtaagactg ttgtacacta ccggggcctt
    10801 ttggccccgt ttttttatct ggaggattaa tgacaatcac ggacctggta ctgattcttt
    10861 tcatcgccgc actcctggcc ttcgcgatct acgatcagtt catcatgccc cgccgtaacg
    10921 gccccaccct gctggcaatt cctttgctcc ggcgtggtcg catcgatagc gttatcttcg
    10981 tcggattgat tgtcattctt atctataaca acgtcacgaa tcatggcgcg ctaataacaa
    11041 catggttatt aagtgcactg gctctgatgg gtttttatat attctggatc cgcgttccga
    11101 agatcatctt taaacaaaaa ggttttttct tcgccaatgt ctggattgaa tatagccgaa
    11161 tcaaagcgat gaacttgtcg gaagatggcg tgttggtgat gcaattagaa caacgtcggc
    11221 tgttaatccg cgttcgaaat atcgacgatc tggaaaaaat ttataagctt ctcgtttcaa
    11281 ctcaataagt tatgaattta gccaaagcta tgtttagtgt atttttaata atcagacata
    11341 gcttaggcta tattatctct tcccttattt gttatttatt ttaacgtttc attgatatat
    11401 aaatctaaat gaaaatcgtt atcaataaag caatgaaata atatattcca acagttgttt
    11461 tatattctca aaatatgtta aggttgcgcc ctcatttggg gagtagccga tttccagatt
    11521 ccggaaatgt acgtgtcaac atactcgttg caaaacgtgg cacgtacgga ctgaagactt
    11581 tcagtcaggc gagaccatat gcacatcaat cgctatgcct gcatgaacgc gaaaacccat
    11641 cgcggcttgt tgttcatgta tgcatgtttg ctgggggcga tgatgtgttt tatggatacc
    11701 ccggtcagga cgttgtcatg aatatcactg ctactgttct tcttgcgttt ggtatgtcga
    11761 tggatgcatt tgctgcatca atcggtaaag gtgccaccct ccataaaccg aaattttctg
    11821 aagcattgcg aaccggcctt atttttggtg ccgtcgaaac cctgacgccg ctgatcggct
    11881 ggggaatggg catgttagcc agccggtttg tccttgaatg gaatcactgg attgcgtttg
    11941 tgctgctgat attcctcggc gggcgaatga ttattgaggg ttttcgtggc gcagatgatg
    12001 aagatgaaga gccgcgccgt cggcacggtt tctggctact ggtaaccacc gcgattgcca
    12061 ccagcctgga tgccatggct gtgggtgttg gtcttgcttt ccttcaggtc aacattatcg
    12121 cgaccgcatt ggccattggt tgtgcaacct tgattatgtc gacattaggg atgatggttg
    12181 gtcgctttat cggctcaatt attgggaaaa aagcggaaat tctcggcggg ctggtgctga
    12241 tcggcatcgg cgtccagatc ctctggacgc acttccacgg ttaataagaa cgctgccaga
    12301 ggtgaatatt aaagtccgtc tggcagtcga acacttcttt tgctgccagt gtttgccaga
    12361 cttctggctt cgcacgccag gcaaacggcg tcatctgcaa taatgcgacg gcttcatcac
    12421 cgcgaagacg catcggataa cacaactcat cactctgctg taatgtaaaa ccttccagtt
    12481 gttctgcatg aggtgcatga agatgtactt cattgtaaat cagccccttc agctccatca
    12541 aatgtcgcgg tcccggcgtg gcagtaatga cccagccgcc gggcttcact actcgtgcta
    12601 attcttctgc tttacacggc gcgtaaatac gtattatggc gtccatactg gtatcggaaa
    12661 acggcaaacg gtggctggaa gcgacacaaa gagtgacctg cggatagcgt ttcgccgccg
    12721 cttttatcgc taccttcgaa acatccagac caaacgtggt gatttcgggc aacgcatcgg
    12781 caaatgcgtg tgtgtaatac ccttcaccac agccaatatc cagcaccgcc gtggcctttt
    12841 catcaagccg ttcccgcagt tgggcgacaa ttgcatcacg cagcggctga taatgtccgg
    12901 catctaagaa tgcgcggcgt gcttgcatca tttccgcgct gtcgcccgga tcacgagacc
    12961 gtttatgctg aacgggcagc agattgacat acccttcttt cgccatatca aactgatgtc
    13021 gttggggaca gatatagctg tttttttcac gcgaaagagg ctgatggcaa agtggacagg
    13081 aaaacgacat gacaactccg gcaggatact aaaggccgca agtgtaacgc gaatcacgcg
    13141 gcgggcaaat atccgaacgc caggagcgaa gataaagcat agtaaaaagc ctcgcattcg
    13201 acgaggcttt atagcgagat tgaagcgtat tcacacttca gatcagtgga ttcgatcaga
    13261 tagctgttac gttaacagct gccggacctt tctggccgtc ctgaatttcg aactcaacgt
    13321 tctgaccttc agccagagtt ttgaagccat taccctggat agcggagaag tgtacgaaca
    13381 cgtctttgct gccatcagcc ggagtaatga agccaaaacc tttagactcg ttgaaccact
    13441 taacctgacc tttaatcttt gccatttgaa aaattcctta gattgttttc ttcgcccgca
    13501 ggcataacat agataaaact gacacattac tgcatgaggc accaatataa ggctcggcag
    13561 agaagcggta ttcaacgtca acgtgtttac tcaggacttc tttactgaaa atgccacaca
    13621 taaacagaac tgtacctcgt ttaacccgaa atctgttatc acatacaacg ttaattatgg
    13681 caagccattt ttaaacatgt ctcgatcaga cgcacaaatc tcgatacgtc ttccactttt
    13741 tttgcacact tatgcaacgg aatacgcgcc gatatatcat tgtgcttaac cttgccagtt
    13801 caggcagata cttaaactgg cgtagtttct aacatagttc aataagttca gttgttccag
    13861 gccagtggga gaagttatta catagtgcgt gcaatatcac at
//
LOCUS       DAPGRN010000122        13778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPGRN010000122 DAPGRN010000000
VERSION     DAPGRN010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.124"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            142..639
                     /locus_tag="RHW33_003868"
     CDS             142..639
                     /locus_tag="RHW33_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HDV1647325.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRDVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCI
                     SIKKWGQNQLKDQYDGSEKLDFGGIEQTPTIYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            671..1402
                     /locus_tag="RHW33_003869"
     CDS             671..1402
                     /locus_tag="RHW33_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HDV1647326.1"
                     /translation="MKTKKLMMVTLVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1655..3808
                     /gene="traD"
                     /locus_tag="RHW33_003870"
     CDS             1655..3808
                     /gene="traD"
                     /locus_tag="RHW33_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDV1647327.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQNPDIQQKMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(3817..4215)
                     /gene="vapC"
                     /locus_tag="RHW33_003871"
     CDS             complement(3817..4215)
                     /gene="vapC"
                     /locus_tag="RHW33_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV1647328.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(4215..4442)
                     /gene="vapB"
                     /locus_tag="RHW33_003872"
     CDS             complement(4215..4442)
                     /gene="vapB"
                     /locus_tag="RHW33_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV1647329.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            4524..9794
                     /gene="traI"
                     /locus_tag="RHW33_003873"
     CDS             4524..9794
                     /gene="traI"
                     /locus_tag="RHW33_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDV1647330.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEVFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMAP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVQIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            9814..10560
                     /gene="traX"
                     /locus_tag="RHW33_003874"
     CDS             9814..10560
                     /gene="traX"
                     /locus_tag="RHW33_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDV1647331.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAEAMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            10619..11479
                     /locus_tag="RHW33_003875"
     CDS             10619..11479
                     /locus_tag="RHW33_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1647332.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            11582..12139
                     /locus_tag="RHW33_003876"
     CDS             11582..12139
                     /locus_tag="RHW33_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV1647333.1"
                     /translation="MTEQKRPVLTLKRKTEGETPIRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            12294..12497
                     /locus_tag="RHW33_003877"
     CDS             12294..12497
                     /locus_tag="RHW33_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647334.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            13240..13701
                     /locus_tag="RHW33_003878"
     CDS             13240..13701
                     /locus_tag="RHW33_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV1647335.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRGVRVLDGNTIDVMLSQHPVR
                     VRLVNIDAPEKKQEYGRWSEKIMKSLVAGKTVTVTYFQRDHYGRILGQVYAPDGMNVN
                     QFMVRAGDAWVYEQYNTDPVLPVLQGEVRQQKRGLWADSAPVPPWVWRSRK"
BASE COUNT         3375 a         3207 c         4098 g         3098 t
ORIGIN      
        1 gagtcattat ctcagaataa taaatataat gaagaaaagt cggctcagga aagaatgccc
       61 ggagctgaca gtccacagga actgatgaaa agggcaaagg agtatcagga taagtataag
      121 caataatact gtgtttggga gatgataatg agatgtttag cccatattgc tctggtaaca
      181 gtgatacagt ttatcgcctg ttatctggct gattggggga atgccgaaac agtttttatg
      241 ctgttcttca tagtgctatg gcagggactg tttatctggt tgttctctca aatacgaaaa
      301 aaacgggatg tgtcggatga gtttaagttt agtaaaggcg tttggtatat aacgatgcca
      361 gtatcttcct tactctctcc gttattaagc ctgatggttt ttatcattgg tacactatac
      421 gaattaaggc gagttagtgg ttgcataagc ataaaaaaat gggggcaaaa tcaactcaaa
      481 gaccaatatg atgggagtga aaagttagac ttcgggggca ttgaacagac cccaacgatc
      541 tactataacc cttcaaccgg atatcctatg catggaggct ttgattcagc gggaaatact
      601 tttgggactc gttggcagga ctattatgac aggcaataag aaaactgtag tttgtaaaaa
      661 aggtaatttg atgaaaacaa aaaaattgat gatggttaca ctggtcagtt ccactctggc
      721 cctttcaggg tgtggtgcga tgagcacagc aatcaagaag cgtaaccttg aggtgaagac
      781 tcagatgagt gagaccatct ggcttgaacc cgccagcgaa cgcacggtat ttctgcagat
      841 caaaaacacg tctgataaag acatgagtgg gctgcagggc aaaattgctg atgctgtgaa
      901 agcaaaaggg tatcaggtgg tgacttctcc ggataaagcc tactactgga ttcaggcgaa
      961 tgtgctgaag gccgataaga tggatctgcg ggagtctcag ggatggctga accgtggtta
     1021 tgaaggcgca gcagttggtg cagcgttagg tgccggtatc accggatata attccaattc
     1081 tgccggtgcc acactcggtg tgggccttgc tgccggtctg gtgggtatgg ctgcagatgc
     1141 gatggtggaa gatgtgaact ataccatgat cacggatgtg cagattgcag agcgtactaa
     1201 ggcaacggtg acaacggata atgttgccgc cctgcgtcag ggcacatcag gtgcgaaaat
     1261 tcagaccagt actgaaacag gtaaccagca taaataccag acccgtgtgg tctcaaatgc
     1321 gaacaaggtt aacctgaaat ttgaagaggc gaagcctgtt ctcgaagacc agctggccaa
     1381 atcaatcgca aatattctct gattatcttc cggaggctgg tctgaccggc ctcctgactg
     1441 acgcctcgct ttgctcgttg tccaaccccg gggagaaata aaataaattt tatttctccc
     1501 cggcgtttcc gaaataaata tgaaaacact gagcggttgt gcaaattctt tgtgcgaccg
     1561 tgaagtgttg ccggcgaagc cggaatttaa cggttattaa tcttttcatc attaataatc
     1621 agtccgactg actttctttc ttcagaatat catcatgagt tttaacgcaa aggatatgac
     1681 ccagggcggt cagattgcgt ccatgcgtat ccgcatgttc agtcagatcg ccaatatcat
     1741 actttactgc ctgtttattt ttttctggat cctagttggt ctggttttat gggtaaaaat
     1801 aagctggcag acgtttgtga atggctgcat ttactggtgg tgcaccacgc tggaaggcat
     1861 gcgggattta atcaggtccc agccggtcta tgagatccag tattacggca aaaccttccg
     1921 gatgaacgcg gctcaggtac tgcatgataa atatatgatc tggtgcggtg agcagttatg
     1981 gtcggcattc gttctggcgt ctgtcgtggc cctggttatc tgcctgatca ccttctttgt
     2041 tgtctcctgg attctggggc gtcagggtaa acaacagagc gaaaatgaaa tcacaggtgg
     2101 tcgtcagctg actgacaatc cgaaagaggt ggccaggatg ctgaaaaaag atggcaagga
     2161 ttccgatatc cggattggcg atctgccgat tatccgggat tctgaaatcc agaacttctg
     2221 tctgcacggc actgtcgggg ccggtaagtc ggaggttatc cgtcgtctgg ccaactacgc
     2281 ccgtcagcgt ggtgatatgg tggtgattta tgaccgttcg ggggaatttg ttaaaagtta
     2341 ctatgatccc tccatcgata aaatcctgaa tccgctggat gcacgctgtg ctgcctggga
     2401 tttgtggaag gagtgcctga cacagccaga ttttgataat accgcaaata ctctgatccc
     2461 gatggggaca aaagaggacc cgttctggca gggttcagga cgtaccattt ttgcggaagc
     2521 ggcatacctg atgcgtaatg accccaaccg cagctacagc aaactggtgg acacactgct
     2581 ttccatcaaa attgaaaagc tgcgtacctt cctgcgtaat tcaccggcgg ccaacctggt
     2641 ggaagagaaa atcgagaaaa cggccatatc catccgtgct gtgctgacca actacgtgaa
     2701 agccatccgt tacctgcagg ggattgagca taacggtgag cccttcacca tccgtgactg
     2761 gatgcgtggt gtccgggaag atcagaaaaa cggctggctg tttatttcgt cgaacgccga
     2821 cacccatgcc tccctgaagc cggtgatctc catgtggctg tccattgcta ttcgtggtct
     2881 gctggcaatg ggagaaaacc gtaaccgtcg tgtgtggttt ttctgtgacg agttacccac
     2941 gttacacaaa ctgccggacc tggtggagat cctgccggaa gcccgtaagt tcggtggctg
     3001 ttatgtgttt ggtatccagt cctatgccca gctggaagat atctacggtg agaaagcggc
     3061 tgccacgctg tttgacgtca tgaacacccg tgcctttttc cgttctccca gccataagat
     3121 tgcagagttc gctgccggtg aaattggtga gaaagagcac ctgaaagcca gcgagcagta
     3181 ttcctacggt gctgatccgg tacgtgacgg ggtatcgacc ggtaaggata tggagcgtca
     3241 gacgctggtc agttattccg acattcagtc tctgccggat ctgacctgtt atgtcaccct
     3301 gcccggaccg tatccggcag taaaactctc cctgaaatat cagacacgac cgaaggtcgc
     3361 tccggagttt attccgcgtg acatcaaccc ggaaatggag aaccgtctga gtgccgtact
     3421 tgccgcaagg gaagcagaag gtcgtcagat ggccagcctc ttcgaaccgg atgtcccgga
     3481 ggttgtttcc ggagaagacg tgactcaggc tgaacagccg caacagccgg tgtctcctgc
     3541 catcaacgat aagaagtcag atgcaggtgt gaatgttccg gcagggggga tcgagcagga
     3601 gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga tcagtgaatc
     3661 cggtgaagtg gtggatatgg ccgtttatga ggcatggcaa cgggagcaga atccggatat
     3721 ccagcagaag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg agcgcgggga
     3781 ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt caattctcag
     3841 accgtccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagtc cccgactgcg
     3901 ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac gggcaagttc
     3961 tgctcttatc tggccgctgt gtgtggctgc ggcagcgtcg taatccagaa catcaatgcg
     4021 ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt tttctgcacc
     4081 atatatcagc tccatcaggg tgaccgaact gatacacatt ctcccctggt tcaggttaaa
     4141 acgctccctg acgctggcgg gtttgttctt tatcgtgaaa atgcagatgt tggtatcgag
     4201 cataaacttc agcatcagaa tgactccctt tcctgcatgc cgggctgttc cctgttatcc
     4261 ataaaatcgg cgctgacgtt gtgtccgtcg aaccattcgt cccatgtctc tccggctggc
     4321 gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt ttccggcaat
     4381 gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgaaaaatac ggtggtttcc
     4441 attatttgcc tccttatgtg gatatgtctt gtgtatatcc attgtgggat tgataccggg
     4501 atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg gccggaagtg
     4561 ccggtaacta ttataccgac aaggataatt actatgtgct gggcagcatg ggagaacgct
     4621 gggccggcag gggggctgaa cagctggggc tgcagggcag cgtcgataag gatgttttta
     4681 cccgtcttct ggaggggaag ctgccggacg gagcggattt aagccgcatg caggatggca
     4741 gtaataagca tcgtcccggc tacgacctga ccttctccgc ccccaaaagt gtctccgtga
     4801 tggccatgtt aggcggtgat aaacgtctta ttgatgcaca taaccaggcc gtggattttg
     4861 ctgtccgtca ggtggaggcg ctggcttcca cacgggttat gacggacgga cagtcagaaa
     4921 cggtgctgac cggtaatctg gtgatggcac tgtttaacca cgacaccagt cgcgatcagg
     4981 aaccacagtt acacacacat gcggtagtgg ccaatgtcac gcagcataac ggcgagtgga
     5041 agacactgag cagtgacaaa gtggggaaaa cggggttcat tgagaatgtg tacgctaatc
     5101 agattgcctt cggcaggctc taccgggaaa aactgaaaga gcaggttgag gcgctgggct
     5161 atgaaacgga agtggttggt aagcacggta tgtgggagat gccgggcgta ccggtggagg
     5221 ccttctccgg acgctcacag gctatccggg aggccgtcgg ggaagacgcc tcgctgaaat
     5281 cccgggatgt ggcggcactg gatacgcgaa aatccaaaca gcacgtcgat cctgaagtca
     5341 gaatggctga atggatgcag acgctgaagg aaaccgggtt cgacatccgg gcgtatcgtg
     5401 acgcggcgga ccaacgggcg gagacccgca cgcagacgcc cggacccgct tcacaggacg
     5461 ggccggatgt gcagcaggcg gtgacacagg ccattgccgg attaagtgaa cgcaaagtgc
     5521 agtttacgta cactgacgtg ctggccagaa cggtcggcat actgccgccg gaaaatggtg
     5581 tgattgaacg ggcacgcgcc ggtatcgatg aggccatcag ccgtgagcag cttatccccc
     5641 tcgatcgtga gaaggggctg ttcacgtccg ggattcatgt gctcgatgag ctgtcagtcc
     5701 gggcactcag tcgtgacatc atgaaacaga accgggtgac cgtacatccg gagaaaagtg
     5761 tcccccggac ggccggttac agcgatgcgg tcagtgttct ggcacaggat cgcccgtcgc
     5821 tggctattgt gtccgggcag ggcggtgcag ccgggcagcg tgagcgggtg gcggagctgg
     5881 tcatgatggc ccgggagcag gggcgggagg tgcagattat cgccgctgac cgtcgctcgc
     5941 agatgaacct gaagcaggat gaacggctgt ccggtgagct gataaccgga cgtcgtcagc
     6001 tgcaggaggg catggccttc acgccgggca atactgttat cgttgaccag ggcgaaaaac
     6061 tctccctgaa agagacgtta accctgctgg acggtgccgc acgtcataac gtacaggtcc
     6121 tgataaccga cagcgggcag cgaaccggta caggcagtgc actgatggcc atgaaagatg
     6181 ccggggtgaa cacatatcgc tggcaggggg gagaacagcg gccggccacc atcatcagtg
     6241 aaccggaccg taatgtccgc tatgcccggc tggcaggaga ttttgctgcc agcgtgaaag
     6301 ccggagaaga gagcgtggca caggtcagcg gggtacggga acaggccata ctgacacagg
     6361 ccattcgcag tgagctgaaa acacagggcg tgctcggacg cccggaggta accatgaccg
     6421 ccctgtcgcc ggtctggctg gacagccgga gccgttatct gcgggatatg taccgtccgg
     6481 ggatggtgat ggagcagtgg aacccggaga cacgcagtca tgaccgctat gttatcgacc
     6541 gggtgacggc gcagagtaac agcctgaccc tgcgggatgc gcagggtgaa acgcaggtgg
     6601 tgcgtatttc ctccctggac agcagctggt cgctgttccg gccggaaaaa atgccagtgg
     6661 cagacggcga gcgactgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg
     6721 accgcctgca ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccggggcggg
     6781 ctgaaccggc caccctgcct gtgagcgatt caccattcat ggcactgaag ctggagaacg
     6841 gctgggtgga aacgcctggg cattccgtca gcgacagtgc gaaggttttt gcctccgtca
     6901 cacagatggc aatggacaac gccaccctga acggtctggc ccgcagcggt cgtgatgtcc
     6961 ggctgtattc ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct
     7021 ttacggtggt ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta
     7081 tcagtctgca gaaaaccggg cttcacacgc cggcacagca ggctattcat ctggcgcttc
     7141 ctgtggtgga aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt
     7201 cgtttgctgc agaaggaacc agttttactg aactgggagg ggaaatcaat gcgcagataa
     7261 aacgcggtga tttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt
     7321 cccgtgcgtc gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg
     7381 cggtcacgcc actgatggag agagtacctg gcgaactcat ggagaaactg acatcaggac
     7441 agcgtgccgc tacccgcatg atactggaaa cgtccgaccg tttcacggtg gtgcagggct
     7501 atgccggtgt gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc
     7561 cggagagtga acgtccccgt gtcgtggggc tggggcccac gcaccgtgcg gtcggtgaga
     7621 tgcgcagcgc cggcgtggat gcacagacgc tggcgtcctt tctgcatgac acgcagctgc
     7681 tgcagcgcag cggagaaacg ccgaatttca gcaacacgct gttcctgctc gatgagagct
     7741 ctatggtggg caataccgac atggcacggg catacgccct gattgcggcc ggtggcggtc
     7801 gtgccgtggc cagtggtgac acggaccagt tgcaggccat cgcgcccggt cagcctttcc
     7861 gtctccagca gacgcgcagt gctgccgatg tggccatcat gaaggagatt gtgcgccaga
     7921 cgccggagct gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa agggcactgt
     7981 ccgggcttga gagtgtgaaa ccgtctcagg tgccacgtca ggagggcgca tgggtaccgg
     8041 agcactccgt gacggagttc agtcacagcc aggaagcgaa actggcagaa gcgcagcaga
     8101 aggcgatgct gaaaggtgag gtttttccgg atattcccat gacactgtat gaagccattg
     8161 tccgcgatta taccggcagg acgccggaag cacgggagca gacgctgatt gtcacgcatc
     8221 tgaatgagga ccggcgcgta ctgaacagca tgattcatga tgcacgggaa aaggccggtg
     8281 agctgggaaa agagcaggtc atggcgcctg tcctgaacac agcgaatata cgcgacgggg
     8341 agttgcgtcg tctctccacc tgggagacac atcgggacgc acttgccctg gtggataatg
     8401 tgtatcaccg gattgccggt atcagcaagg atgacgggct gataaccttg caggatgcgg
     8461 aaggtaacac gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc acactgtaca
     8521 ctccggacac catccgggtg ggaaccggtg accggatgcg cttcacgaag agtgaccggg
     8581 agcgcggtta tgtggccaac agcgtctgga cggtgacagc ggtttccggt gacagtgtca
     8641 cgctgtcgga cggacagcag acccgggtga ttcgccccgg tcaggagcgg gcagagcaac
     8701 atattgacct ggcctatgcc atcaccgccc acggtgcgca gggggcaagt gaaacctttg
     8761 ccattgcgct tgaaggcacg gaaggtaacc ggaaacagat ggccggtttt gagtcagcct
     8821 acgtggccct gtcgcgtatg aagcagcatg tgcaggtgta taccgataac cgtcagggct
     8881 ggacggatgc cattaacaat gccgtacaga aaggaactgc ccacgatgtg ctagagccga
     8941 aatcggaccg ggaggtcatg aatgcagagc ggctgttcag tacggcgcgg gagctgcggg
     9001 acgtggtggc cggccgtgcc gttctccgtc aggcagggct ggccggggga gacagtcctg
     9061 cacggtttat tgcacccggg cgcaaatatc cgcagccgta tgtggcactg ccggcttttg
     9121 accgtaacgg caagtcagct ggtatctggc tgaacccgct gaccacggat gacggaaacg
     9181 ggctgcgggg cttcagtggt gaaggccggg tgaaaggcag cggggatgcg cagttcgtgg
     9241 ccctgcaggg cagccgtaac ggagagagcc tgctggctga taatatgcag gatggtgtcc
     9301 agattgcccg tgataatcct gacagcggtg tggtggtaag aatcgccggt gaaggtcgtc
     9361 cgtggaatcc cggtaccatc accggtggtc gcgtgtgggg ggatatcccg gacaacagcg
     9421 ttcagccggg agccggaaat ggcgaaccgg tcacggcaga ggtactggca cagcggcagg
     9481 ctgaagaggc catccgccgt gaaacggaac gccgtgcgga tgaaattgtc cgtaaaatgg
     9541 cagagaacaa acctgacctg ccggacggca aaacagagca ggctgtcagg gatattgccg
     9601 ggctggagcg tgacaggtct gccatatctg aacgggaagc tgcgctgccg gagagtgtac
     9661 tgcgtgaacc acaacgggtg cgggaagcgg tccgggaggt tgcccgggaa aatctgctgc
     9721 aggagcgact gcagcagatg gagcgggata tggttcgtga cctgcagaaa gagaaaaccc
     9781 tgggcggaga ctgacacagg aagataaacg ctgatgacaa cggataacac gaacacaaca
     9841 cgtaatgatt cactggctgc ccggaccgat acctggttgc agtcgttgct ggtctggtca
     9901 cccggacagc gggatatcat caaaacggtg gcactggtgc tgatggttct ggaccatgca
     9961 aaccgcattc tgcatctgga ccagtcgtgg atgtttctgg tcgggcgtgg ggcctttccg
    10021 ctgtttgccc tggtgtgggg gctgaatctg tcccgtcata cgcatatccg gcaggaggcc
    10081 attaaccggc tgtggggatg ggcggtgatt gcccagttcg catattatct ggccggcttt
    10141 ccctggtatg aggggaatat cctgtttgcc tttgcagttg cggcacaggt gctgacatgg
    10201 tgtgaaacgc gcacctggtg gcgttccgca gaggccatgt tgcttctggc aatgtggttg
    10261 cctttctccg gcaccagtta cggcattgcc gggctgctga tgctggcagt cagccacagg
    10321 ctgtaccggg cggaagacag aatggaacgt ctggcgctgg tcgcctgcct gctggctgtt
    10381 attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggcct tgtgatgacg
    10441 gtgctgactg ttggtctggt gtcctgtaca gggaaatcac taccccgttt ctggtacggg
    10501 gattttttcc cgacgttcta tgcctgtcat cttactgtgc tgggcgttct ggctgtgtga
    10561 ctggtatgat gctggcgctg attctgctta ttaataaaca acataacaca ggatatttat
    10621 gaaaattacg gatcacaaac tccctgaagg tattgccctg acatttcgtg tcccggaagg
    10681 aaatataaaa catcctttga ttctcttatg ccatggattt tgcggtatcc ggaatgttct
    10741 gctgccttcc tttgcgaatg cgtttacaga agccgggttt gcaacgatta cttttgatta
    10801 tcgtggattc ggtgagagtg aaggggaacg aggtcgtctg gttccggcaa tgcagaccga
    10861 agatattatt tcagttatca actgggcaga aaagcaggta tgtattgata atcaacgtat
    10921 aggattatgg ggaacatcgc tgggtgggtg tcatgtattc aatgcagctg cgcaggataa
    10981 aagagtgaaa agtattgtca gccagctggc ttttgctgac ggtgaggttc tggttaccgg
    11041 agaaatgaat gaactggaga aagcttcttt tctgtccacg ctgaataaaa tggcagaaaa
    11101 gaagaaaaat accgggaaag agatgtttgt gggggttacc cgggtactga gtgataatga
    11161 atcaaaggta ttcttcgaga aggtaaaagg tcagtatcct gaaatggata taaaaatacc
    11221 tttccttacg gttatggaga cgttgcaata taaaccggca gagagcgcag ccaaagtaca
    11281 gtgcccagtt ttggttgtta ttgccggcca ggatagtgtt aatccaccag aacagggaag
    11341 agccttatat gatgctgttg cttctggtac taaggaatta tacgaggaag ctgacgcatg
    11401 ccattatgat atttatgaag gcgcgttttt tgaacgtgtg gcggcagtac agacgcaatg
    11461 gtttaaaaaa catctttaat tgtcggtatt aatatgtcag aggtcgttga ggtcagctta
    11521 atatcatagt gagtagtttc aacgacctgg aggacatatt ttcacaaagg tcagggcaat
    11581 catgacagag cagaaacgac cggtactgac actgaaacgg aaaacggaag gggaaacacc
    11641 gatccggagc cggaaaacca tcatcaatgt caccacgcca ccaaaatgga aggtgaaaaa
    11701 gcagaaactg gcggagaagg ctgcccggga agcagagctg gcggcgaaga aagcacaggc
    11761 cagacaggcg ctgtccattt atctgacgct gccctcgctg gatgaggcgg tgaacactct
    11821 gaaaccctgg tggccgggat tgtttgacgg taacacgccc cggcttcttg cctgtggtat
    11881 ccgggaagtt ttactggatg aggtgtctca gcggaatatc ccgctctctc ataaaaaact
    11941 gcgcagggca ctgaaggcca tcacccgttc agaaagctat cttggcgcca tgaaagccgg
    12001 tgcctgccgc tatgatacag aaggctatgt gacagaacat atcacacagg aagaagaaca
    12061 gtatgcgcag gcgcgcctgg aaaaggtgcg cagacagaac cggatcaagg atgagctgcg
    12121 ggcgatcctg gcagaataat aaaagcgccc tctgtatgac agagggcaac agcgaaaaaa
    12181 atcgggaaaa cagacatcca gagttttgat ggagaaaaac atccaaccgt tcagggatgt
    12241 ttcataatgt ttctgacata atccgggtgt cgggtcaaga ggagggataa aaaatggctg
    12301 tatccagtgg attatctgaa cacagcagta tttatcgcta tcagcgcgag gggcgtcatg
    12361 ccgtccgtca ggagattcgt ggctttttca gggaagcggc agaggtctgg cagaatgcgg
    12421 cccggctggc accctgtgaa gcatggcggg attttgctct gaagagggca tctgcatgtc
    12481 ggtgtaagtg ccgataaaaa taacccccgt cgcggagctc cgggggatca gacttatatg
    12541 gcagatcgtt attattatta tagtggtgta agaaggagtt gccctgtctt tcaaacggac
    12601 atcacagaat taacatattt cacatcccgg gtaagccagc agggataaaa aatgtgatga
    12661 atttcaagaa gcaggaggtg acagtggcat gcataacagt agggttatcc ggtgaatctg
    12721 acagacagcc gggctgtatt cgcggcagga taaagaaaaa ccgggagatg actctcccgg
    12781 tccgtcttat cgctaatccc ctgagcaggg catgtatgac ttatctatga ccgttatatt
    12841 cagcgccaca tccggtagcc tgcgatataa cacagttatc ctgtcagcaa gagcaggtat
    12901 gatgaataca cggagattaa aaatataatc tccgtcacat gttacccacg gaaaccggta
    12961 acgccttcac gggaaatatg tgacagaaaa agcagcctcc gttactccag gtatcaggag
    13021 gctgaataat gcatgagacg ttgtttctta tgtgtctggt caggctggct gaccagcgtt
    13081 tttagtaaca catagccata tgtgtattaa gtcacctcat cttattttgt tgaattgatc
    13141 acaactcaac ggtttatttt ttgcgtgata cgtgagccat tattcatccc ggcatggaat
    13201 tcatgcaatc acgaaaagta atcagtgacg gagagtaaaa tgaagaaata tataccagtg
    13261 gtattatttg ttttttcatg tcaggtattt tctgctgata ttcatggacg gggcgtgcgt
    13321 gttctggatg gcaataccat tgacgtcatg ttatctcagc atccggtgcg tgtccgtctt
    13381 gttaatattg atgcacctga aaagaaacag gaatatggac ggtggtctga aaaaataatg
    13441 aaatctctgg ttgcgggaaa aacggttact gtcacatatt ttcagcgaga tcattatggt
    13501 cgtattctcg ggcaggttta tgcaccagac ggaatgaatg ttaatcagtt tatggtccgt
    13561 gcaggcgatg catgggttta tgagcagtac aacaccgatc ctgttcttcc tgtcctgcag
    13621 ggagaagtac gacagcagaa acgtggtctg tgggcagaca gcgctcctgt tccgccctgg
    13681 gtatggcgct cacggaaata atgatatttc tgtggctatt gagttctcat tgaaatgcga
    13741 tatttaaaag catgaagcat attacattat taacttat
//
LOCUS       DAPGRN010000123        13398 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPGRN010000123 DAPGRN010000000
VERSION     DAPGRN010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.125"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..845
                     /locus_tag="RHW33_003879"
     CDS             15..845
                     /locus_tag="RHW33_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV1647336.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDNELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(986..1759)
                     /gene="uxuR"
                     /locus_tag="RHW33_003880"
     CDS             complement(986..1759)
                     /gene="uxuR"
                     /locus_tag="RHW33_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV1647337.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1974..3434)
                     /locus_tag="RHW33_003881"
     CDS             complement(1974..3434)
                     /locus_tag="RHW33_003881"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV1647338.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGCWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3515..4699)
                     /gene="uxuA"
                     /locus_tag="RHW33_003882"
     CDS             complement(3515..4699)
                     /gene="uxuA"
                     /locus_tag="RHW33_003882"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV1647339.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5039..6382
                     /gene="gntP"
                     /locus_tag="RHW33_003883"
     CDS             5039..6382
                     /gene="gntP"
                     /locus_tag="RHW33_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV1647340.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6556..7458)
                     /gene="fimH"
                     /locus_tag="RHW33_003884"
     CDS             complement(6556..7458)
                     /gene="fimH"
                     /locus_tag="RHW33_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV1647341.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7478..7981)
                     /gene="fimG"
                     /locus_tag="RHW33_003885"
     CDS             complement(7478..7981)
                     /gene="fimG"
                     /locus_tag="RHW33_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV1647342.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7994..8524)
                     /gene="fimF"
                     /locus_tag="RHW33_003886"
     CDS             complement(7994..8524)
                     /gene="fimF"
                     /locus_tag="RHW33_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV1647343.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8534..11170)
                     /gene="fimD"
                     /locus_tag="RHW33_003887"
     CDS             complement(8534..11170)
                     /gene="fimD"
                     /locus_tag="RHW33_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV1647344.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCI
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11237..11962)
                     /gene="fimC"
                     /locus_tag="RHW33_003888"
     CDS             complement(11237..11962)
                     /gene="fimC"
                     /locus_tag="RHW33_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV1647345.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(11999..12538)
                     /locus_tag="RHW33_003889"
     CDS             complement(11999..12538)
                     /locus_tag="RHW33_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1647346.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12603..13151)
                     /gene="fimA"
                     /locus_tag="RHW33_003890"
     CDS             complement(12603..13151)
                     /gene="fimA"
                     /locus_tag="RHW33_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1647347.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALALDGVTFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3253 a         3463 c         3226 g         3456 t
ORIGIN      
        1 aaaaggtaac tattatgtct atgccattaa gcaataccct tcagagtcaa attattacag
       61 ataatcactt ccttcatcac ccaaaagtcg acaatgagtt aacacgtcga tatgaatatg
      121 cacgcctgga tacggaaaat atctatcttc tgcctcttgc tcgtggcaat aaccataatt
      181 acgatggaaa aagcgttgtc gaaattagaa aacttaatat tggcaaacag tcctggccat
      241 ttaattatgt gacagataca tgcagagaat tcaatggtat tacaacaaca ggacgtatgc
      301 tctatcgaaa cttgaaaatc acctcagctt tagacgaaat atatggtggt atatgtaaaa
      361 aggcgcatgc tgctacagag ttagcagaag gtttacggct aaatttattt atgaagagcc
      421 ctttcgatcc tgttgaggac tatacagttc atgaaataac attaggccct ggttgcaatg
      481 ttcctggtta tgccggaacc acgataggat atatttcaac ccttccagct tctcaggcaa
      541 aaagatggac caatgagcag ccaagaattg atatctatat tgatcagata ataactgtct
      601 caggggtagc aaattcttcc ggattcgccc ttgccgcatt acttaatgct aatattggaa
      661 tgggaaatga cccaataatt ggtatagaag cctatccagg taccgcggag atccatgcaa
      721 aaatgggata taaagttatc cccggtgatg aagatgcgcc actcaaacgc atgactctgc
      781 aaccgtcatc tcttccagaa ctgtttgaat taaaaaatgg tgaatggaat tatattggta
      841 aatagccact gagaagaatg agtactaata ttcaggttat acagatattt aaacaatacc
      901 ctgcaattta caaagtaata ctcattgcga cttcttaatt taagaaagcg caaggaacgt
      961 ttacccttgc gcttattata ataagtcagg cgtcgacttt atccagcggc catgaatcaa
     1021 acagatagcc gtcaaaataa atatcgtcaa cgttcgagaa ttccagcaga cgttgcttaa
     1081 cgttttccag atgctgccac attgccagct tcgccgctcg ggcatctttt ttgattaacg
     1141 cggcgaggat ctgtttgtga tcgcccaacc actctttgcg atacaggctg tcatccagat
     1201 ggctgtgcaa ctggatccac attggattgt tttcccgcca ctgccaggac tgacggaata
     1261 actccaccag catgctgtta tgcgttgctt cagcaatggc gagatggaac tgcatgtcac
     1321 cgctttcgct gctacccggc gcgctggaag ccagttcacg ctcttccagt tgcaatgcct
     1381 gacgcatttt gacgatatct tcgcgggtag cctgcaaagc ggcaaattcc gcgatgttac
     1441 tttccagtaa ctgacgcgcc tgtaacagct caaaaggacc cgcatcattg cagacgttgg
     1501 catccggact gtctgtgttg tggctgcctg agctatcaag aacatagata ccggcacccc
     1561 ggcgcacttc caccagccct ttgatctcca gcatgatcag cgcttcacgt accaccgtcc
     1621 gcgtgacatc aagcatttct gcaatttcac gctccggcgg tagccgttca ccaggattgt
     1681 acggcgtctt tatgatcaga tcgcggatca tcgccccgac ttcctggtaa ggtctttgcg
     1741 cagaggtggc agatttcatg gtgttgtgtc cgttaggtgc agttaatcaa atttttgccg
     1801 gatgcggcgt gaacgcctta tccggcctac aacgggcaat tttacggccc ctgttaatct
     1861 caaactatac cattagcact ggaagttggc acgtacaggt gggcaaaaaa acgaaaactg
     1921 gtcaaccaaa tatcagtttg attgaccagt aagtctgtag ggtaatcatt tagttacagc
     1981 gcagccacac actcgcgcgc accgcgttcg cacagctgct gatatgccgc cgtcaccgcg
     2041 ccaacaaagt cggcattctg cggcagatca tcggcaaaaa taccgctcag gcccagcagc
     2101 gctttcacgc ggtctgcgcc ctgatactgc gcgttaatct tctggaactc ggccagcatc
     2161 gggtcgacca cgtcaatggc attaccctgc tcatccacgc cctgggtgta acgcatccag
     2221 ccagccacgc ccagcgccag gtgacgccag cagccgccgt tttgcaggtg cagacgcacc
     2281 gggtccagca gacgctgcgg taacttctgg ctgccgtcca tcgcaatctg ccaggtacgg
     2341 tgacgcagag acgggttgct gaaacgctcg atcagcagcg tcgcataggc gttcaggtcc
     2401 gtaccctccg gcatcgacag cgttggcgct tgttcctgca tcatcagggc aaaggccgct
     2461 ttgcgataat ccgggttagt catggtgtcg gcaatagttt catagccgcc gaggtaaccg
     2521 aggtacgcca ggaaagagtg gctgccgttc agcatacgca gcttcatcat ttcgaacggc
     2581 acaacgtctg caacgaactg tgcgcccact ttatcccagt ccggacgacc attaacgaag
     2641 ttatcttcaa tcacccactg acggaacggt tcgcaggcaa tggcgcacgg gtcgtaaaca
     2701 cccagctgat cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac gatgcggtca
     2761 accatggtgc acgggaaggt gacgttctct tcaatccatg ccgctagctg cgggtcacgc
     2821 gcctgagcca gccccagtac cgcgaccttc gccacatgac cgttttcacg cacgttgtcg
     2881 caggacatca ccgtaaacgc tttcagccct ttttcacgac gcagacgcaa ggcttcgacg
     2941 atgtaaccaa tcgcggactt cggcgcagtc gggttttcca gatcgtgctt gatcagcggg
     3001 ttattgagat ccagctgacc gcttgccgca tcagcgcagt agcctttttc cgtgaccgtc
     3061 agagagacaa tcgccgtctg cggtcgtgcc atcgcgttaa gaataccttc gcagccgtcg
     3121 atttccggat gcagagcttc tttcatcgaa ccgataattt tcagctcggt gctttctgcg
     3181 cctttttccg ctacggtgta cagcagttgc tgttttttca ggttttcgat cagcacgcgg
     3241 tcgttgcctg gcatcaggtt aacttcgcag atgccccagt cgctgtcggt gctttccagc
     3301 agatgatggg tatacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc gagatgcaca
     3361 atgcgtgatt ccagacgaga atgatcccat gacgggcggg caaccggcag attgctgtca
     3421 acaatagtag tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc aaggaagaag
     3481 gaaccggcac ggcgcgcagc catgccggtg gatattaacg gctaaagaaa gcgcgctgga
     3541 tcgccagttc gacaccgcga acttcggcca ggcctttcag acgaccaatt gcggagtaac
     3601 ctgggttggt tttcttcttc aggtcgtcca gcatctgatg accgtggtcc ggacgcatcg
     3661 ggatcaggtc ttctttgcct tccgctttac gacggtgttc ttcttcaaca atcgctttca
     3721 ccacttcgta catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag gttttcgggt
     3781 tatcttcacg catggtggag cgcagatggg tgaagtaaat acgcggaccg aactgcttga
     3841 tcatatcaac cagatcgttg tcagcacgca cgccgtagga accggtgcac atggtgaaac
     3901 cgttcgccat gctgtttacg gtatcaacca tccactgcat atcttcaatg gtggaaacaa
     3961 tgcgcggcag gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc atacgcacgc
     4021 caacttcttc agcaactgga ataatcgctt tcaggaagac ggcaaagttt tcgcgcagct
     4081 tcgctttatc gatatctttg tacagctcca ggtgcttacg gaactggtcg agggtatacc
     4141 cttcttccgc gcccggcaga ccggcaatga tgttacgcgt cagacgcgct ttgtcttcat
     4201 cgctcatagt ggcgaagcgt tcagctgcct gagcaatttc ttcttcggtg taatccgctt
     4261 ccgcgcctgg acgtttcagg atatgcattt cgaatgcagc gaattcgatc tggtcgaagc
     4321 gcagagcttt ggagccgtct ggcagcacgt attcgaggtc agtacgggtc cagtcgagca
     4381 ccggcatgaa gttgtagcac acggtgcgaa tgccgcactg cgccaggttg cgcagagtct
     4441 gctgatagtt agcgatccac tgctcatagt tgccggtgtg ggttttgata tcttcgtgaa
     4501 ttggtacgct ttctacgaca gaccacacca ggcctgcgtc ttcaacgatc gccttgcgtt
     4561 tgaggatctc ttctacggac catacttcgc cgttcgggat atggtgcagc gcggtaacca
     4621 cgccagttgc gcccgcctga cggacatcag ctaaagaaac cggatcgttt gggccgtacc
     4681 agcgccaggt ctgttccatg atttttcctc tttcacatag tgggttccac agcaaagaga
     4741 cgcggaaccc tggagttgtt taggtatccg gatgaacaac cagaccgcag ccgtggcgtt
     4801 gcatcggatg agatggcgta taagttctac cttttggtaa gacatgtcaa cccgaattct
     4861 aaaattggtt aaccacatca caagaatttc actctcatgg ttgttgctgc aaattgcagc
     4921 aaagggtaat tgatgtttat tgatcagagg atgtgacatt catcgcaaca atggttgacc
     4981 aatttacata acatgtcgcc caaaataaca acggttcaac caggtaaggt aaaacgttat
     5041 gcatgtgctt aacattctct gggtggtatt cggcattggt ctgatgctgg tactgaattt
     5101 gaagttcaaa atcaattcaa tggtggcttt gttggtggcg gcgctgtccg tcgggatgct
     5161 ggcgggcatg gatttgatgt cgctgctgca caccatgaaa gcgggcttcg gcaacacgct
     5221 gggggaactg gctatcatcg tggtgttcgg tgcggtcatc ggtaaattga tggtcgactc
     5281 cggcgcggct caccagatag cgcatacgct gctggcgcgt ctcggtctgc gctatgtaca
     5341 gctgtcagtg attatcatcg gcctgatttt tggtctggcg atgttctatg aagtggcctt
     5401 tatcatgtta gcgccgctgg ttattgttat tgccgccgaa gctaaaattc cgttcctgaa
     5461 actggcgatc ccggcagtag cagctgccac taccgcacat tcactgttcc caccgcagcc
     5521 gggtccggtg gcgctggtga atgcttatgg cgcagatatg gggatggttt atatctatgg
     5581 cgtactggtg acgatcccaa gtgtaatctg cgcaggtctg atcctgccga agttcctcgg
     5641 caatcttgag cgcccaacgc catcattcct gaaagcagat caaccggtag atatgaacaa
     5701 tctgccctct ttcggcgttt cgattctggt gccgctgatc ccggcgatca ttatgatctc
     5761 taccaccatc gccaatatct ggctggtaaa agatacccct gcctgggaag tggttaactt
     5821 tatcggttcc tcgccgattg caatgtttat tgcgatggtg gttgcattcg tactctttgg
     5881 caccgcacgt ggtcatgaca tgcagtgggt gatgaacgct tttgaaagtg cggtgaaaag
     5941 cattgcaatg gtgattctga tcatcggtgc gggtggcgtg ctgaagcaaa ctatcatcga
     6001 caccggcatt ggcgacacca tcggcatgtt gatgtcccac ggcaatatct cgccctacat
     6061 tatggcatgg ctgatcactg tgctaattcg tctggcgacg ggtcagggtg tcgtttcggc
     6121 gatgaccgcc gccgggatta tcagtgctgc aatccttgat ccagcaaccg gtcagctggt
     6181 tggcgtgaat ccggcgctgc tggtgctggc gacggctgcg ggttccaaca ccctcaccca
     6241 cattaacgat gcatctttct ggttgttcaa aggttacttt gacctgtcgg taaaagacac
     6301 gttgaaaacc tggggtctgc tggagctggt caactccgtg gttgggctga ttattgtgct
     6361 gattattagc atggtagcgt aaaagaacac cagagcctgc caatggcagg ctcagttcgg
     6421 attgtcggca aagtgcgctt tgtttatgcc agatgcgacg ctgacgcgtc ttatctggcc
     6481 tacaaagggc taacgtgcag gtttttagct tcaggtaata ttgcgtacct gcattagcaa
     6541 tgccctgtga tttctttatt gataaacaaa agtcacgcca ataatcgatt gcacattccc
     6601 tgcagtcacc tgccctccgg tacgtgcgta atttgccgtt aatcccagac ttaccgccga
     6661 agtccctact gctcctaacg ataccgtgtt attcgctgga ataatcgtac cgttgcgcgt
     6721 caactgtacg ccgacgccct gcgctggtga aaacgacgcg gtattggtga aaatcgagtt
     6781 gcccgcatct gcggttgtgc cggagaggta ataccccagg ttttggcttt tcgcacaata
     6841 aacggtaaga ggaattggca ctgaaccagg gtagtccggc agagtaacgg tgacatcacg
     6901 agcagaaaca tcgcagccgc cagtaggcac caccacatca ttattggcgt aaatattcca
     6961 cacaaactgg aaatcatcgc tgttatagtt gttggtctgt cgcaaaataa gcacggcaat
     7021 taatgaacca gctttaatcg ccaccccgcc cgcactgctc acaggcgtca aataaagcgc
     7081 caccggccac ggcttatccg ttctcgaatt ataaacaacc cgcggcgttt cgctggtggt
     7141 cggaaatgga tagctactgc cactatattt tacggtcccg gaaaaattag ataacacgcc
     7201 gccataagcc gagcctcgtt gcagtgtgac atagtctgta atggtttccg gataatcgtt
     7261 atggcaaaag atttgcgtcg aaagatctac gaccaggttt tgccccacat tcacggcagg
     7321 cgcaaggttt acataaacat tagcgctgcc accgccaata ggaatagcgg taccattggc
     7381 ggttttacag gcgaatgacc aggcatttac cgaccagccc atcagcagta cagcaaacag
     7441 ggtaataact cgtttcatta caattatctc ttcgggttca gctgtaggta taggtgatgc
     7501 taatcactgc ctgaatggtt ccctgagtgg ctccgccatt taccgtcaat gctctgacct
     7561 gtaacgggaa gtgcgctgat tgtgaggaat catccacctg aactgttttg gttgcgccag
     7621 tattcaatgt gttgccactg tcatcctgta gctctaactg gatgttttgc gcggtcccct
     7681 ggtttttata atatccggta ctgtcggctg ccccgctgaa gctggcagtg accctcgacg
     7741 ttcccaccgg acaattagtc aactcaagcg caacatcatg ccaggccgat gccgccccgg
     7801 cagacatcag actgaaagaa taaagatcgc cgagatcaac cgtggcattg gtggtggaaa
     7861 ctgtgcacgg tttggcgacg accttaccgt tcaccgtgat ggtgacatcg gctgcctgta
     7921 tcgtcgcact tgcgagcgcc aatattgccg ccaataaata cccacgtttg cgccatttca
     7981 tgagcatctc cagttactga tattcaagag tgaaggtagc cgtggcattg atatgccccg
     8041 cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa attcagcgta tttggtttac
     8101 ccggcgtcag ggtcgtccac gaaatcgcgg acgatggagc attaaggggt atttgatttt
     8161 gctgctcatt cagaagctgt attcccaatc ccgaagccgc tgacaccgta ttttcaagtg
     8221 caagcaggtt ggcattgtgg ctatctgcaa cgccggtaaa cccaaccttt acggcagaaa
     8281 cggcgttacc acagggtgac agcaaaatac gaaatggaac gacaggagtc gttgcgccaa
     8341 tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac agtaaaattg gttgattcag
     8401 cggccacact acagccgtta tctctgacat agccgcggat agtaatcgtg ctatccgcag
     8461 ccaaaacgcg acttaccgcc agccacaaaa aagcgcacag aagataaaaa ggtttgtttc
     8521 tcatcacgcc cccttaacga cattcagctg atagctgggt taataactgc tgctgactct
     8581 ctggtggcag ttgataattg gcgatacagt gagcattttc ctcttctccc catttcacct
     8641 gaacttttcc cgctaaaggc attccgctga ggtaaacctg accattatcc gcaacaatgc
     8701 cgctactctg gctactctct gatgtcacca tcgccccaaa cggcagcggc ttattattgt
     8761 gggttagcgt catgagcagt tttatcccaa cgcgcgcttt aaactctgct cgcacgatcg
     8821 ccccacgagt gggaacaacg ttagcgaccg cgttatctaa atcgacgtta tcagccaggg
     8881 tattggtatc cagcgccact ctgttttccc gatattcggt ggcataaggc agcacggcat
     8941 aaccgcgcca gtcggtacgc acccccgtct ggttttcgac ttttgcatct tttgcgccag
     9001 gcgctttaac aagtaccacc gtatcgttta acggctgccc cagcgttacg ccattggcat
     9061 gagccagtac cccaccgctg actccgtaat agagctgctt aatatcatcg ctatggctgt
     9121 aaccgatatt ggcattgccg taaccaccgc gataattcag cgtggcgtag cctgtacttc
     9181 cgctattacc atcgcctccc ccggcatagc cggtttgcac gctatagctg aggttgttgt
     9241 cttccagcaa cgtaccgtat acgccagcca gattggtcat ccgaccgttg aggtcgtgtg
     9301 acatgctgta gctggcactg gcatgtcgcc actgagattt actgtcagaa cgcaaccagt
     9361 ggctgaaagg aatattgacg ttaagcgcta acatctgatc ccgtcctttt tgccaggcgt
     9421 ttttcgtcag gctatagctg agcgtccagt tgatatcttc gaacgcagta tttaatccag
     9481 cctggaattg ctcatcgaca ttactcgttc cccaataagt ttgatggcta ccactcaaat
     9541 acagtgttga tgtgcgcccg agttgctgag taacggtgag ctgtaatttc ccgcgtttgt
     9601 tataagcgag gttgtaatag tcggtgaatt tcggcttaac ctgaataact ccatcctgtg
     9661 tttcgatgtt gtagccattc attcgactgt atgttgtatc agcgaaatta aaatatccgc
     9721 tggtcgaata acggtaaccc actaactgaa tattcgtgcc tgactcattg agcgatttgt
     9781 tatagagaaa acgcaccgat tgtccgtcat gctgactgtc atcgggaagt gtggaattag
     9841 cctgagtcat atccacagac agagcgccta gtgcccccat atttttcccg ataccaaaat
     9901 taaaagcacg ataacgatct gccagttgcg ttccaccata tattgtccag ccagccggaa
     9961 ggccgtggag taatgtactt tggaaaaagc ggggtttttc ctgttgcgca tttccactac
    10021 ggtattctcc tgccgtaatg gaataacgag tatgcccttc acgttgcaaa agcgggactg
    10081 acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc ctctttaatc gttacctgca
    10141 agtcaccact attacctgcg gcatagatat cgttgatggt aaaaggcccc ggcggcaccg
    10201 tactattata aatgtcatac ccattttgtt taatagtgac ctgtgcagta ccacgagcaa
    10261 taccgtggat caccggggca aatcctcttt ggctatcggg taacatattg tcatctgagg
    10321 ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc gccctgggta taaccatcac
    10381 ccagcgtcag ccgggaacgt aaaggaatta tgtctcgctc aagccaggta ttgatatgct
    10441 gccatttatt tttgctacct gatgaactgt cgctactgtt ataactccag gtggtattgt
    10501 cgcgtaaacg ccacgcacca atatttaacc cactctgtag gtttaaatat gcataatggc
    10561 tgttaccccc aatccgattc tgtacactat tcccgctgaa attataattg agcaatccgg
    10621 cattaatacc gggatcccat aactcaggag gaatataacc acgcgcgcga ttactcataa
    10681 atgcctgagg gatcgtcagg ttcagtcgct gctgaccaac atctaaatgc gcagtagcgt
    10741 cctggaccat tgtggttaat ggcacacagg catcatccgc cagcagattc ataccggcga
    10801 cagaagccgt attcagcccc atactggcga gttgcgcgcg tgtcaggcag ggaacaatcc
    10861 cttgttcact gtcgcccgta ttaaatgtga catcgcgcgt tgccatataa ccattattca
    10921 aatagatatc gacgcgatac gtccctggcg gtaattcttg cccattttca aaacgcgata
    10981 aatcggccac agcctgggga tcatccgcta aaaagcgcgg attaaaatag agttcggcag
    11041 atgacaaagg tgcctgtgcg gcaaaagcac aggcgacaaa gagccggaca aaaaaaccag
    11101 ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg ctggtaaagt cttaaattca
    11161 gatatgacat tattcctccg gtttaaaatc acccggcagt caattctttt tattcaggcg
    11221 aaaaattccc cctgcgttat tccattacgc ccgtcatttt gggggtaagt gcgccataat
    11281 catttattgt tcggtaagta atattgcttc ctgcatcaga aggcaattta acagtgcttt
    11341 cgcccattgg aggcaccaag gcattttcaa gaacccgggc tccggcattc aactctgtta
    11401 ccgtcaggta atagggtgtc gggttaatca gcgtcagaga attcgcgcta cgacgaaatc
    11461 ttaatttttc tgcggcctga tcgggtggca acgctaattt agccggacga tagtacagtt
    11521 taatgcggct gataattgcg agctgtagcg tattctcagt caattttgat ttatccattg
    11581 acggaatcgc tttaacgttc atccagaata aactttcccg gtcctgtggc aattggttat
    11641 ttgttgcatc aagaatacgc aaggtattct ctttttttcc cttcatcgca aacagaggag
    11701 gcgtcacgat aaaacgacca tcctttacac catcggcatt ttccacccat gattggatta
    11761 aataggtact attttcatca ttatttgtca cggcaagttg cacttgtttt tgccctgccg
    11821 gataaattac gcgagtcgca cctaaggcca ctcccgcttc agcgcgtccg gcaaccatca
    11881 ttgccatgaa catcaggata cctgccagca agcagaatgt tatttcctgc gatttcctta
    11941 cattgacgtt tttattactc actgtcctgt tcctgttatc acattatctg ctgaacaatt
    12001 actgataggt taaagagaac caggcctgtg cattggcgat gccgccagta acccgacgcc
    12061 cggtagcacg atatttggcg atgaaatgta gcgaagtaga gcctgagtaa agccgtttcc
    12121 agtttgctgg aggacgatta atcggtacga ggtttccttc atcatcaaac aacgctacgc
    12181 caatattggt ggctatccct ggcccctctc ccacggaaag cacatccgga tttttaccat
    12241 ccgcgacacc gtgaaacgcc acacctacac gttcactcac caccgtgcta cattcccgta
    12301 aatgaataac aaaaggcacc ggtgcgctat cttcccctac cgcatgaaac cggttactgc
    12361 tgatttgccc catattgacc gtcatttgtt tatcaccggc ttcaatccgg caagtttccg
    12421 caataatgac gccctgaaat tgcatatttc cgccgggcaa cgtggtattc catttatttc
    12481 cggccagagc aaacattggc agcaacgacg ctaatagaaa taatctcttc cttttcatcg
    12541 ttatttttat cgcacaaggg tgggcatccc tgcccgtaat gacgtccctg aacctgggta
    12601 ggttattgat actgaacctt gaaggtcgca tccgcattag cagcacccgg ggttgcggcc
    12661 ccggttgcaa aataacgcgc ctggaacgga atggtgttgg ttccgttatt cagggttgtt
    12721 tctgaactaa atgtcacacc gtccagcgcc agcgcagcac ccgttctgtc caggatctgc
    12781 acaccaacgt ttgttgcgct acccgcagct gaactctgca gagccagaac gttggtatga
    12841 cccgcatcaa tcgccgtacc taaaaaggca acagcggctt tagatgcaac attggtatcg
    12901 caatcattca gctgaatgtt aaaaccgaca gcagaactgg ttgctccttc ctgtgccagc
    12961 gatgcggtac gaacctgtcc taactgaacg gtttgatcaa cagagcctgc atcaactgcg
    13021 caagcggcgt taacaacttc ccctttaaag tgaacggtcc caccattaac cgtcgtggca
    13081 gcggccagag ccgctgtaga actgagggac agagccgaca gaacaacgat tgccagagtt
    13141 ttaattttca tgctgctttc ctttcaaaaa actatttcta aatcgacatg ggcagtcgtt
    13201 ctgtacactt tgttttgtca acgagtttga ctgccaacac tgcacagttt tcccccaaaa
    13261 gatgaaacat ttggggccat tttgactcat agaggaaagc atcgcgcaca aactttttca
    13321 gtttatttgt tggcttaata ttctattgtt atatttattt atagattttt atattgcatg
    13381 aggtggtttt tggagaga
//
LOCUS       DAPGRN010000124        13261 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPGRN010000124 DAPGRN010000000
VERSION     DAPGRN010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.126"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..163)
                     /gene="rprA"
                     /locus_tag="RHW33_003891"
     ncRNA           complement(56..163)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RHW33_003891"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(349..1461)
                     /gene="ydiK"
                     /locus_tag="RHW33_003892"
     CDS             complement(349..1461)
                     /gene="ydiK"
                     /locus_tag="RHW33_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV1647348.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1850..4906
                     /locus_tag="RHW33_003893"
     CDS             1850..4906
                     /locus_tag="RHW33_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV1647349.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGLRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4903..5313
                     /gene="menI"
                     /locus_tag="RHW33_003894"
     CDS             4903..5313
                     /gene="menI"
                     /locus_tag="RHW33_003894"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV1647350.1"
                     /translation="MIWKRKITLGALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGQVRGVC
                     KPLHLGSRHQVWQIEVFDEKGRLCCSSRLTTAIL"
     gene            5413..5601
                     /locus_tag="RHW33_003895"
     CDS             5413..5601
                     /locus_tag="RHW33_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV1647351.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6150..6518
                     /gene="sufA"
                     /locus_tag="RHW33_003896"
     CDS             6150..6518
                     /gene="sufA"
                     /locus_tag="RHW33_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV1647352.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAVHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6527..8014
                     /gene="sufB"
                     /locus_tag="RHW33_003897"
     CDS             6527..8014
                     /gene="sufB"
                     /locus_tag="RHW33_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV1647353.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            8024..8770
                     /gene="sufC"
                     /locus_tag="RHW33_003898"
     CDS             8024..8770
                     /gene="sufC"
                     /locus_tag="RHW33_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV1647354.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPGLCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8745..10016
                     /gene="sufD"
                     /locus_tag="RHW33_003899"
     CDS             8745..10016
                     /gene="sufD"
                     /locus_tag="RHW33_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV1647355.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGVKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSMLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            10013..11233
                     /gene="sufS"
                     /locus_tag="RHW33_003900"
     CDS             10013..11233
                     /gene="sufS"
                     /locus_tag="RHW33_003900"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV1647356.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11246..11662
                     /gene="sufE"
                     /locus_tag="RHW33_003901"
     CDS             11246..11662
                     /gene="sufE"
                     /locus_tag="RHW33_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV1647357.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11811..12815
                     /gene="ldtE"
                     /locus_tag="RHW33_003902"
     CDS             11811..12815
                     /gene="ldtE"
                     /locus_tag="RHW33_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV1647358.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGLRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12879..13115)
                     /gene="lpp"
                     /locus_tag="RHW33_003903"
     CDS             complement(12879..13115)
                     /gene="lpp"
                     /locus_tag="RHW33_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV1647359.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         3324 a         3263 c         3571 g         3103 t
ORIGIN      
        1 tcttgtgcaa cattattgga aagagtgagg ggcgaggtag cgaagcggaa aaatgttaaa
       61 aaaaagccca tcgtgggaga tgggcaaaga ctacacacag caattcgttg tttcactcag
      121 gggatttcca tgcttataaa tcaatatgtt gatttataac cgtgagctaa tagtaggcat
      181 acggactttt tgcgtcgatc agattcgtct cattagttta aaaactgtaa agaatttgcg
      241 acaggtacta ccagctgaat ggcggtaagc aggaaaatat ggggaagagc aagcaaatat
      301 caattagcgg tggttaacaa tcaggcggca gagccgcctg atgagaaatt acttattcgg
      361 tttctcgatt tcgccaagtt cttcgagaat ttcttccggt tggtctgtcg gcggcgggac
      421 ttcttccacc cacgcggcaa acagacgcca ggaaacggct aacagaaccg gaccaataaa
      481 cagaccgatc atcccgaaag caatcaaacc accaataacg ccagagagga tcaggatcag
      541 cggtaaatcg gcacccatgc gaattaacat tgggcggatg acgttatcca gcgtgccaac
      601 cacaccgctc cacactaaca ataccgttcc ccaggtggta tcgccggtcc agtagagcca
      661 gataatcgcc ggaatcagta ccggcaacgg gccaagctgg acaaggcagg agaggatcat
      721 taacaccgtt agcaacgttg cataaggtac gccggatacg gcgaggccga taccgccaag
      781 cactgcctgt actaacgccg tcaccaccac acccagcgcc accgcgcgga tagcctgtgc
      841 cgccagcagt acggcggcat ctccgcgaac gcctgccaga cgggtcgcaa aatggcgaat
      901 gccttgtgcc acctgttcac cgcgccagta cagcagggca ctgaagagaa gcatcagcgc
      961 acaatgcacc ataaagcgcc cgatatgcgc cgcttgccca acgaaccagg tggtggtggt
     1021 gccaatataa gggcggactt tcgccatgat cgccgtcccc cccatatcca gcaagttgtg
     1081 ccaccctgca tacagcttcg cgccaatcac cggaatggta ttaagccacg ccagatcggg
     1141 taacgtcatg tcaccgctgg aaatggcttt aataagcggg ccgctgccgt cgacgatact
     1201 gttaaccagc aaagcaatag ggatgataaa caccatcact aataacagcg tcatcaccag
     1261 aacggcgaga gagcggcggc caaacatgat cttttgcaaa cgtaacaata ccggccaggt
     1321 ggcgataacc accgtaccgg cccatgcaaa gccgagaata aagggttgaa caatccacag
     1381 acatgccaca atcatgatgg ctaaaaacag caccgaaagc agaatttgtg cgacatccct
     1441 gggctgacga acatttacca taactacttt tcaccttttt tgtgcgtcaa atcgtcgacg
     1501 cgacgtgaac acgcgaaact cacctgcata atgatgagca atttcagcgg tttttaacag
     1561 gccgattctg cccttaattc tgatgggcgt gttagaaaaa aatgtggtac aacaactcag
     1621 gcaacacgca aacgatttac tcgtcgtatt tcaagttgca tatgctgcgg ctacgttcgt
     1681 tcaccccagt tacttactgt tgtaagctcc tggagttcac tcgcttgccg ccttcctgca
     1741 actcgaaatc catagagtaa ataaacatca cctttctgtg cgctattgca gccgcctgaa
     1801 agaacagtgt atcgacctac atcacagacc gcaggaaagg gtcaatataa tgattccaca
     1861 gatttcccag gcacccggcg tcgttcaact ggtgcttaat tttttgcaag agctggagca
     1921 acaaggtttt accggcgata cggcgacaag ttatgccgat cgtctgacaa tgtcgaccga
     1981 caacagtatt taccaacttc tccccgatgc ggtggtattt ccgcgttcaa ccgcagatgt
     2041 ggcgctgatc gcccgtcttg ccgcgcagga acgctattca tcgctgatct ttaccccccg
     2101 cggcggcggc accggcacta acggtcaggc gctcaaccag gggattattg ttgatatgtc
     2161 ccgccatatg aaccgcatca tcgaaattaa ccctgaagag ggctgggtgc gcgttgaggc
     2221 cggggtgata aaagatcaac tcaatcagta cctgaaaccg ttcggctact tttttgcgcc
     2281 ggaactttcg accagcaacc gggcaacgct cggtgggatg atcaataccg atgcatccgg
     2341 tcagggatcg ctggtctatg gcaaaacgtc agatcacgta cttggcctac gtgcggtatt
     2401 gttggggggc gatattctcg atacgcaacc tttacccgtc gagctcgcgg aaacgctggg
     2461 taaatccaat accacaatcg ggcgaattta taacacggtt tatcaacgtt gccgtcagca
     2521 acgccagtta attatcgaca acttccccaa acttaaccgc tttcttaccg gttacgatct
     2581 gcgtcatgtc tttaacgatg agatgaccga gttcgacctg acgcgcattc tgacgggttc
     2641 agaagggacg ctggccttta ttaccgaagc gcggctggat attacgcgct tgcctaaagt
     2701 gcgccgtctg gtgaatgtca aatatgactc ttttgactct gcgctgcgta acgcgccgtt
     2761 tatggttgag gcgcgggcac tttcggtaga gacggtggac tcaaaagtgc tgaatctggc
     2821 gcgggaagat attgtctggc attccgtcag cgagttgatt accgatgtgc ctgacaaaga
     2881 gatgctcggg ctgaacattg tggaatttgc tggtgatgat gaggcgctga ttgacgagcg
     2941 ggtaaatgca ctctgtgcgc ggttggatga gctgatcgcc agtcatcaag caggtgtcat
     3001 cggctggcag gtgtgccgtg agctggcggg ggttgaacgt atctatgcaa tgcgcaaaaa
     3061 agccgttggt ctgcttggca atgccaaagg tgccgctaag ccaattccgt ttgctgagga
     3121 tacctgcgta ccgccggaac atctggccga ttatattgct gaatttcgcg cgctgctcga
     3181 cagccacggc ttaagctacg gtatgttcgg tcacgtcgac gcaggtgtct tgcacgtccg
     3241 tccggcactg gatatgtgcg atccgcaaca agagattttg atgaagcaaa tctctgatga
     3301 cgtggtggcg ctgactgcga aatacggtgg tttgttgtgg ggcgagcacg gcaaaggttt
     3361 tcgcgctgaa tacagcccgg cgtttttcgg ggaggaactt tttgcagaac tgcgcaaagt
     3421 gaaagcggca tttgacccgc ataaccgact caacccaggg aagatttgcc cgccagaagg
     3481 tctcgatgcg ccgatgatga aagtggacgc ggtgaagcgc ggtacattcg atcggcagat
     3541 ccccattgcg gtacgccagc agtggcgcgg tgcgatggag tgtaacggca acggtttatg
     3601 cttcaacttt gatgcccgta gtccgatgtg tccgtcgatg aagatcaccc agaaccggat
     3661 tcattcaccg aaagggcgcg caacgctggt gcgtgaatgg ctgcgtttgt tggcggatcg
     3721 cggcgttgac ccactcaaac tggaacaaga actgcctgaa tccggcgtca gtttgcggac
     3781 gttaattgcc cgcacgcgca atagctggca tgcgaataaa ggcgaatatg acttctcaca
     3841 cgaagtcaaa gaggcgatgt cgggctgtct ggcctgtaaa gcgtgttcga cccagtgccc
     3901 catcaaaatt gatgtgccgg agtttcgctc tcgttttctg cagctctatc acacccgtta
     3961 tttacgcccg ctgcgcgacc acctcgttgc tacggtcgag agctacgcac cgctgatggc
     4021 acgcgcgccg aaaaccttta acttcttcat taaccagccg ctggtgcgca aactctcgga
     4081 aaaacatatc ggcatggttg atttgccgct gctttcggtc ccctcgctac aacaacaaat
     4141 ggtggggcat cgctcggcaa acatgacgct ggaacagctt gaagctctca atgcagagca
     4201 gaaagcgcgc acggtgttgg tggtgcagga cccctttacc agctattacg atgcgcaagt
     4261 ggtggcggat tttgtccgtc tggtcgaaaa attaggtttc cagcctgtgt tactgccatt
     4321 ttcgccaaat ggcaaagccc agcatattaa aggttttctt aatcgttttg cgaagacggc
     4381 gaaaaagacg gcggatttcc tcaaccgtat ggcgaagctg ggtatgccaa tggtaggcgt
     4441 cgatccggcg ctggtacttt gttatcgcga tgaatataaa ctggcactgg gcgaggaacg
     4501 tggcgagttt aacgtcttac tggcgaatga atggctggca agcgcacttg agtcacagcc
     4561 agtggctaca gtcagcggtg aatcatggta tttctttggt cactgtaccg aggttaccgc
     4621 cttgccgggc gcgccagcac aatgggccgc gatatttgcc cgttttggcg cgaaactgga
     4681 aaatgtcagc gtgggttgct gcggcatggc agggacttac ggacatgaag cgaaaaacca
     4741 taaaaattcg ctcgggatct atgagttatc ctggcatcag gctatgcagc gactgccgcg
     4801 aaaccgctgt ctggcgaccg gatattcctg ccgtagtcag gtcaaacggg ttgaaggcac
     4861 gggggtacgc catcctgtgc aggctttact ggagattatt aaatgatatg gaaacgaaaa
     4921 atcaccctgg gagcactgaa tgccatgggt gaaggaaaca tggtggggtt actggatatt
     4981 cgctttgaac atattggtga tgacaccctt gaagcgacaa tgccagtaga ctcgcggaca
     5041 aagcagcctt tcgggttgct acatggaggt gcatccgtgg tgctagccga aagtatcggt
     5101 tccgttgccg gttatttatg tactgaaggt gagcaaaaag tggttggcct ggaaatcaat
     5161 gctaaccacg tccgctcggc acgagaaggg caggtacgcg gcgtatgcaa accgttgcat
     5221 ctcggttcgc gtcaccaggt ctggcagatt gaagtcttcg atgagaaagg gcgtttgtgc
     5281 tgttcgtcac gtttgacgac cgccattttg tgacgttgtc accagaaaag tgtgacgcat
     5341 attgcttttg gtcattatct gcacagtaaa cggtggtata ctgtgcaggt cttgtctaaa
     5401 agcgaggatg ctatgtctac tcaacttgat cccacccagc tggccattga atttttacgc
     5461 cgtgaccaga gcaatctttc acctgcccaa tatcttaaac gtctgaaaca actagaactt
     5521 gaatttgctg acctgcttac tctctcttct gctgaattaa aagaagaaat ctactttgcc
     5581 tggcgtctgg gcgtgcattg atcctgactt aatgccgcaa gttcgccaaa tcgacctgga
     5641 ttaacctccg tccaggttcg atgaccttct taatactgct tatctacccg cctgatattg
     5701 ttcaaaaata atactaatcg cagtttgtgt taaaacggcg ggttagcttt atgagattat
     5761 tcttatcgcc ccttcaagag ctaagccact gagagtgccg gagataagcg ccggatgggg
     5821 tagaaaccct taagcctgtg tcgcacagac ttaagggttt tcttatttct gaacgctgca
     5881 cttttttacc tatctcttaa atagtcattt tcaatacaac tttttctttt tcatcacagc
     5941 cttaatgctc cttagataat aatcattatc taacaatgag atacctaatt cttagcgtgc
     6001 ctgttaaccc acacatcagg gtctatgctt attaaaagca ggcaaaaagc atgttttacg
     6061 gtaaagcccc tgcgtttgtt gggttgaact gataatcatt atcactaaca tgctgttata
     6121 cgctgaaagc gatgaagtga ggtaaatcga tggacatgca ttcaggaacc tttaatccac
     6181 aggatttcgc ctggcaaggc ttaacgctga cacccgcagc cgctgtccac atccgcgagc
     6241 tggtggcaaa gcagccgggt atggtcggcg tgcgcttagg tgtgaagcaa acgggctgcg
     6301 cgggctttgg ctatgtgctc gacagtgtta gcgagccgga caaagacgat ctgctgtttg
     6361 aacacgacgg cgcgaagctg tttgtcccgc tgcaagcgat gccgtttatt gatggcacgg
     6421 aagtcgattt cgttcgtgaa ggacttaatc agatattcaa atttcacaac cctaaagccc
     6481 agaatgaatg tggctgtggc gaaagctttg gggtataggc ggtactatgt ctcgtaatac
     6541 tgaagcaact gacgatgtca aaacctggac cggcggcccg ctgaattata aagaaggatt
     6601 cttcacccag ttagccaccg atgagctggc aaaggggata aacgaagagg tggtgcgcgc
     6661 aatttcggcg aagcgtaatg agccggagtg gatgctggag tttcgtctaa acgcctatcg
     6721 cgcatggctg gagatggaag aaccgcactg gttgaaagcg cactacgaca agctgaatta
     6781 tcaggattac agctactact cggcaccatc gtgcggtaat tgtgacgaca cttgcgcgtc
     6841 tgaacctggc gcggtgcagc aaactggcgc gaacgccttt ttaagtaaag aggtggaggc
     6901 ggcgtttgag cagttgggcg ttcccgtgcg ggaaggcaaa gaggtggcgg tggatgccat
     6961 tttcgactca gtttcggttg ccactactta tcgcgaaaaa ctggcggagc agggaattat
     7021 tttctgttcc tttggtgagg cgatccacga tcacccggaa ctggtgcgta aatatctcgg
     7081 caccgtggtg ccggggaatg acaacttctt tgccgcgctt aatgcggcgg tagcctctga
     7141 cggtacgttt atttatgtgc ctaaaggcgt gcgctgcccg atggaacttt ccacctattt
     7201 tcgcattaac gcggaaaaaa ccgggcagtt tgagcgcacc attctggtgg ccgacgaaga
     7261 cagctacgtc agctacattg aaggctgttc cgctccggtg cgtgacagct atcagttaca
     7321 cgcggcagtg gtggaagtca tcatccataa aaacgccgag gtgaaatatt ccacggtaca
     7381 aaactggttc cctggcgata acaacaccgg cggtattctc aacttcgtca ccaagcgtgc
     7441 tttgtgcgaa ggcgaaaaca gcaaaatgtc atggacgcaa tcagaaaccg ggtcagcgat
     7501 tacgtggaaa tatcccagct gcattttgcg cggcgataac tccattggtg agttttactc
     7561 agtggcgctg accagcggtc atcagcaagc ggataccggc accaagatga tccacatcgg
     7621 taaaaacacc aaatcgacca ttatctcgaa agggatctct gccggacata gtcagaacag
     7681 ttatcgcggc ttagtgaaaa tcatgccgac ggcaaccaat gcgcgcaatt tcactcagtg
     7741 cgactcaatg ctgattggcg ctaattgtgg ggcgcatacc ttcccgtatg tcgaatgtcg
     7801 caataacagc gcacaactgg agcacgaggc aacgacatca cgtattggtg aagatcaact
     7861 gttttactgc ctgcaacgcg ggatcagcga agaagacgcc atctcgatga ttgttaacgg
     7921 tttctgcaaa gacgtgttct cagaactgcc gctggaattt gccgttgaag cacaaaaact
     7981 cctcgccatc agtcttgaac acagcgtcgg ataaggaata aacatgttaa gtattaaaga
     8041 tttacacgtc agcgtggaag ataaagctat cctgcgtgga ttaagcctcg acgttcgtcc
     8101 cggcgaagtt cacgccatta tggggccaaa cggttcgggc aaaagtacct tatcggcaac
     8161 gcttgccggg cgagaagatt atgaagtgac gggcggcacg gttgagttca aaggcaaaga
     8221 tttgcttgca ctgtcgccgg aagatcgtgc aggcgaaggc atctttatgg ccttccagta
     8281 tccggtggag attccaggcg tcagtaacca gtttttcctg caaacggcac ttaatgcggt
     8341 gcgcagctat cgcggccagg aaacgctcga ccgctttgat tttcaggatt tgatggaaga
     8401 gaaaatcgct ctcctgaaga tgccggaaga tttattaacc cgttcggtaa acgttggctt
     8461 ttccggcggc gagaaaaagc gcaacgatat tctgcaaatg gcggtgctgg aaccggggtt
     8521 atgcattctt gatgaatcgg actccgggct ggatattgac gcattaaaag tggttgccga
     8581 tggcgtgaac tcgctgcgtg acggcaagcg ctcattcatc attgttacgc actaccaacg
     8641 cattctcgac tacatcaagc ctgattacgt tcatgtgcta tatcagggac gaattgtgaa
     8701 atccggcgat ttcacgttgg tcaaacaact ggaggagcag ggttatggct ggcttaccga
     8761 acagcagtaa cgcactgcaa cagtggcatc atttgtttga agctgaaggg gtgaaacgtt
     8821 ccccgcaagc acaacagcat ttacaacaat tgctgcgtac cggactgccg acacgtaaac
     8881 atgaaaactg gaaatatacg ccgctggaag ggctgaccaa tagccagttt gtcagcattg
     8941 cgggggagat atccccacag cagcgtgatg ccttagcgtt aacgttagac gccgtgcggc
     9001 tggtgtttgt cgatggacgt tacgtgcctg cgctgagcga tgcaactgaa ggcagcggat
     9061 atgaagtgag cattaacgac gaccgtcagg ggttacccga cgctattcag gcggaagtgt
     9121 ttctgcattt gacggaaagc ctggcacaaa gcgtgacgca tatcgccgtg aagcgcggtc
     9181 aacggccggc aaagccattg ctgttaatgc atatcaccca gggcgtggca ggtgaagagg
     9241 tgaacactgc ccattatcga catcatctgg atctggcgga aggtgccgaa gcaacggtga
     9301 tcgaacattt tgtcagcctt aatgatgctc gtcattttac cggggcacgg ttcactatca
     9361 acgtcgcagc gaatgcccac ttgcagcata tcaagctggc gtttgaaaac ccgctcagtc
     9421 accactttgc ccataacgat ttgttgctgg ctgacgatgc caccgcattt agccacagtt
     9481 tcctgctggg tggcgcagtg ttacgacaca acaccagtac gcaactcaat ggcgaaaaca
     9541 gcatgttgcg gatcaatagc ctggcgatgc cggtgaaaaa cgaggtgtgt gatacccgta
     9601 cctggctgga acacaataaa ggtttttgta acagccgaca gttgcacaaa actatcgtca
     9661 gcgacaaagg ccgcgcggta tttaacggtt tgatcaacgt cgcgcagcac gccatcaaaa
     9721 cggatggtca gatgaccaac aataatctgc tgatgggcaa actggcggaa gtggatacga
     9781 aaccgcagct ggaaatctat gcagatgatg tgaaatgcag ccacggcgcg acggtggggc
     9841 gtattgatga tgaacagatg ttctatctgc gctcgcgcgg gatcaatcag caggatgccc
     9901 agcagatgat catttacgcc tttgctgccg aactgacgga agcactgcgt gatgaggggc
     9961 ttaaacagca ggtgctggcc cgaatcggtc aacggctgcc aggaggtgca agatgacttt
    10021 ttccgtcgac aaagtgcggg ccgactttcc ggtgctttcg cgtgaggtaa acggtttgcc
    10081 gctggcttat ctcgacagcg ccgccagtgc gcagaaaccg agccaggtga ttgacgccga
    10141 ggccgagttt tatcgtcatg gctacgcggc ggtgcatcgc ggtattcata ccttaagcgc
    10201 ccaggcgacc gagaaaatgg agaacgtgcg caagcgggca tcgctgttta ttaatgcccg
    10261 ttcggcggaa gagctggtgt tcgtccgcgg cacgacggaa gggatcaatc tggtcgccaa
    10321 tagctggggc aacagcaacg tgcgggcggg cgataacatc atcataagtc agatggagca
    10381 ccacgctaac attgttccct ggcagatgct ttgcgcacgc gttggtgcag agctgcgtgt
    10441 gatcccgctc aatcccgacg gtacgttgca actggagacg ctgcctacgc tgtttgatga
    10501 gaaaactcgc ctgctggcaa ttactcatgt ctccaacgtg cttggcacag aaaatccact
    10561 ggcggaaatg atcacgcttg cgcaccagca tggcgcaaaa gtgctggtgg atggcgctca
    10621 ggcggtgatg catcatccgg tggatgttca ggcgctggat tgcgactttt acgtgttctc
    10681 cgggcataaa ctgtatggcc ccaccggaat tggcattctt tatgtgaaag aagccttgtt
    10741 gcaggagatg ccgccgtggg aagggggcgg ttctatgatc gccaccgtca gcctgagtga
    10801 aggcacaacc tggaccaaag caccttggcg gtttgaagcc ggtacaccca ataccggggg
    10861 catcattggt cttggcgcgg cgctggagta tgtttcggcg ctggggctta ataacatagc
    10921 cgagtatgaa cagaatctga tgcattacgc gctatcacag ctggaatctg taccggatct
    10981 cactctctat ggtccgcaga acaggcttgg cgttattgct tttaatctcg gtaaacacca
    11041 cgcctatgat gttggcagtt ttctcgataa ttacggcatt gctgtgcgta ctggacatca
    11101 ctgcgctatg ccattaatgg cctattacaa cgtccctgcg atgtgtcggg cgtcgctggc
    11161 catgtataac acccatgaag aagtggatcg tctggtgacc ggattgcaac gtattcaccg
    11221 tctgctggga taacagggag gcactatggc tgtattgccg gataaagaaa aattgctgcg
    11281 taatttttta cgctgcgcca actgggaaga gaaatatctc tacattattg agctgggcca
    11341 gcgtctgcca gaattacgcg acgaagacag aagcccacaa aatagcattc agggctgcca
    11401 gagtcaggtg tggattgtca tgcgccagaa tgcgcaggga attattgaat tgcagggcga
    11461 cagcgatgcg gcgattgtga aagggcttat tgcggtcgtc tttattctct acgatcagat
    11521 gacgccgcag gatattgtca atttcgatgt gcgtccgtgg tttgaaaaaa tggcgctcac
    11581 ccaacatctc accccatctc gttcacaagg tctggaagcg atgattcgcg caattcgcgc
    11641 caaagccgct gcacttagct aaactaaagg gacagctttc atccggttgg ctcacattgc
    11701 cggatggtca atgtttacac tgacctgcac aggagtgttt cggtttaagg tgaacataag
    11761 cgccatccga cgtttcagcg tgagtctccg gcaaatacag gaggtttaca atgaaacgcg
    11821 cgtctttgct tacactcacg cttatcggcg cttttagcgc catccaggct gcctgggcgg
    11881 ttgattatcc gctaccacca accggaagcc gactggttgg gcaaaatcaa acgtatacgg
    11941 tgcaggaagg ggataaaaac cttcaggcca tcgcccgacg ttttgatact gcggcaatgt
    12001 tgatccttga agccaataac actatcgccc cggtgccaaa acccggtacg acgataacta
    12061 ttccttcgca actgttatta cctgatgcgc cgcgtcaggg gattatcgtt aaccttgcag
    12121 agctgcgcct ttattattat ccgccgggag aaaatatcgt gcaggtttat ccgataggta
    12181 ttggcttgca ggggctggaa acgccggtga tggaaacgcg tgtggggcag aaaatcccta
    12241 acccaacctg gacgcctact gcaggcattc gtcaacgttc actggagcgt ggtattaaat
    12301 taccgccagt cgttcctgcc ggaccaaata acccgctagg acgttacgca ctgcgcctcg
    12361 cgcatggtaa tggcgaatac ctcattcatg gcaccagtgc gccggacagc gtcggtttgc
    12421 gcgtcagttc agggtgtatt cgcatgaatg caccggatat taaagccttg ttctccagcg
    12481 tgcggacggg aacgccggtg aaagtgatca acgaaccggt gaaatattcc gtggagccta
    12541 acgggttgcg ttatgttgaa gtacatcgac cactatcggc agaagaacag cagaacgttc
    12601 agacaatgcc atacacactg ccagcaggct ttacgcaatt taaagacaat aaggctgtag
    12661 atcagaagtt agtcgataaa gcgttgtatc gtcgggcagg gtatccggtt gcggtgagca
    12721 gtggagcaac tcccacagcc agcaatgcgc cttcagtaga gtcagcgcag aatggcgaac
    12781 cagagcaagg gaatatgtta cgcgcgacgc agtagtggta aatagcagac aaaaaaaatg
    12841 gcgcacaatg tgcgccattt ttcacttcac aggtactatt acttgcggta tttagtagcc
    12901 atgttgtcca gacgctggtt agcacgagct gcgtcatctt tagcagcctg aacgtcggaa
    12961 cgcattgcgt tcacgtcgtt gctcagctgg tcaactttag cgttcagagt ctgaacgtca
    13021 gaagacagct gatcgatttt agcgttgctg gagcaacctg ccagcagagt agaacccagg
    13081 attaccgcgc ccagtaccag tttagtagct ttcattatta ataccctcta gattgagtta
    13141 atctccatgt agcgttacaa gtattacaca aagtttttta tgttgagaat atttttttga
    13201 tgggaaggca cttatttttg atcgttcgct caaagaagca tcgaacgctg ggttttagat
    13261 a
//
LOCUS       DAPGRN010000125        13162 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPGRN010000125 DAPGRN010000000
VERSION     DAPGRN010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.127"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            111..1445
                     /gene="adeQ"
                     /locus_tag="RHW33_003904"
                     /gene_synonym="yicO"
     CDS             111..1445
                     /gene="adeQ"
                     /locus_tag="RHW33_003904"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV1647360.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1498..1950
                     /locus_tag="RHW33_003905"
     CDS             1498..1950
                     /locus_tag="RHW33_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV1647361.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2065..2388)
                     /locus_tag="RHW33_003906"
     CDS             complement(2065..2388)
                     /locus_tag="RHW33_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV1647362.1"
                     /translation="MTAKTKFKSPAFEAIHSAAAGLSSVDAISAETMRTFDKACLTSV
                     QDLQPVEIKALREKLKVSQPVFAHYLNTSVSTVQKWESGAKRPNGISLKLLSIVQKHG
                     LDVLL"
     gene            complement(2372..2725)
                     /locus_tag="RHW33_003907"
     CDS             complement(2372..2725)
                     /locus_tag="RHW33_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1647363.1"
                     /translation="MFKTKWFAREARSHAITDEELCRAILETEQGKADALGGGVFKKR
                     LHQNRERAIILAKGGSNWFYTFLYAKQDMSNINSQELAGFRELAKHYAFLTKAQLTAM
                     VNTKELTEICYDCKN"
     gene            2959..4143
                     /gene="nepI"
                     /locus_tag="RHW33_003908"
     CDS             2959..4143
                     /gene="nepI"
                     /locus_tag="RHW33_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV1647364.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MK"
     gene            4345..12843
                     /locus_tag="RHW33_003909"
     CDS             4345..12843
                     /locus_tag="RHW33_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HDV1647365.1"
                     /translation="MAGKAHGNGDRRGDNTICGLGDRLRRLTAGICLITQTIFPVMAA
                     APTHINHARSDTATSLILPNVKTIPYTLGALESPSTVAARFGITVDELRRLNQFRTFA
                     RGFDNVRQGDEIDVPLINSNSPEARNLKAMQMERDGKDPQMQVAEVAQQSGTLLARDM
                     DSEQAASMARGWVASSASAQATDWLSRWGTARVSLGVDEDFSLKSSSFEFLHPWYETP
                     DNLVFSQHTLHRTDDRTQTNHGIGWRYFTPSWMSGVNMFIDHDLTRYHTRTGMGVEYW
                     RDYLKLSGNGYLRLSNWRSAPELDHDYEARPANGWDLRAEGWLPAWPQLGGKLVYEQY
                     YGDEVALFGKDERQNDPHAITAGLSYTPVPLISFSAEQRQGKQGENDTRIGMELTLQP
                     GHSLQKQLDPAEVAARRSLVGSRYDLVDRNNNIVLEYRKKELVRLTLTDPLKGKPGEV
                     KSLVSSLQTKYALKGYDIEAASLQSAGGKVAVSGKDIQVTIPPYRFTAMPETDNTYPI
                     AVTAEDSKGNFSRREESMVVVEKPTLSLADSTLSVDLQILLADGKSTSTLTYTARDSS
                     GKPIPGMTLKTQAKGLQDFALSEWKDNGNGTYTQIVTAGKTSGALSLMPQFNGDDIAK
                     TPALIAIVANTASRADSTIETDQDNYVAGKPIVVKVTLRDDNGNGVTGRKELLKQTVK
                     VDNTKADAVSAWTEESEGIYKASYTAHLIGDKLTAQLTMPGWQTKHSDAFSIAGDKDT
                     AKIAAMQITANNAVARRDHNTVAVTVRDVHQNLLQGQNVTFTVVNGAAVFADPNGGIV
                     TTDKDGIASVNLASDQAVNSLIKAEINGSSQSVEVSFITGDISQLTSTIKTDDVSYTA
                     GGKIKVSVTLMDEQKNLVKGMASLLAGSGVVEVSGTDKNETGNWSEESDGVYTTTRTA
                     KIAGDRHYATLKLSTWSSAQQSDAYAIRESGAVLAYSSIVTDKTTYTAGGVIKVTVTL
                     KDSYENLVGGQRDAINQAIQLPNTKAESIAWNEDQKGIYTATYTALLPGTGLKAQLQM
                     SDWASALTSNDYSISGDAASAQIVAMQVTTGNPDVLANGSDRHTVNVRVEDQFGNVLP
                     EQTVTFTVTKGAAVFANAGQSADIRTDAHGMAEVDLSSTVADASTVEAKVNQSSDSKT
                     VNFVADVSTAQIADLVVIKDGSVADGSTANTLRVRVTDAFGNALDGQTVSVLADNGAT
                     VSPTVITGPDGTVEISVTSQTAGISAVTASINSSNASRNVTFIADVRTAQIADLVVIK
                     DGSEADGATANTLRARVTDAFGNALAGQTVSVLADNGATVAPTVITGPDGTVEISVTS
                     QTAGTSAVTATINSSSQSRNVTFIADIRTAKIADLVVIKDGSVADGATANTLRARVTD
                     AFGNALAGQTVSVTAGNGATVAPTVITEPDGTVEISVTSQTAGISTVTASINSSSLSR
                     DVTFIADVRTAQIAELVVIKDGSEADGSTANTLRARVTDAFGNALAGQTVSVLADNGA
                     TTAPTVITEPDGKVEISVTSQTAGVSAVTASINNSTLSQNVMFIADIRTAQIAELVVI
                     KDGSEADGATANTLQVKVTDVFGNALAGQTVSVLADNGATVTPTVTTEPDGTVEISVT
                     SQTAGISTVTATINNSTLSQNVTFIADVRTAKIADLVVIKDGSVADGATANTLQVTVT
                     DAFGNTLTGQTVSVLAGNGGTVAPTVITEPDGTVEISVTSQTAGVSAVTASINNSSLS
                     QSVMFIADIRTAQIADLVVIKDGSEADGSTANTLRARVTDAFGNALAGQTVSVLADNG
                     AAVASTMTTKPDGTVEISVTSQTAGTSAVTASINSSSQSRDVTFIADIRTAQIADLVV
                     IKDGSVADGAMANMLRARVTDAFGNALAGQTVSVFAGNGATTAPTVTTQPDGTVEISV
                     TSQTAGVSAVTASINSSSQSRDVTFIADVRTAKIADLVVIKDGSVADGAMANMLQVKV
                     TDANGNVLAGQTVSMMAGNGATVAPTVITEPDGTVEIPVTSQTAGASAVTASINSSNA
                     SRNVTFVADVRTAKIADLVVIKDGSVADGAMANTLQVKVTDAFGNALGGQTVSVTAGN
                     SATVTPTVTTQSDGTVEFSVTSQTAGVSAVTATINNHSLSQNVTFVPGDASQLTSTVE
                     TNKSNYTVGETITITVTLRDAFDNLVTGAASQLAANSVLTVDGTDPSETGSWVESGGV
                     YTTTRMATIAGTDQHANLQLQTWSDGVTSDRYDIQSGSPAQATSTIATDKNAYIAGET
                     ITVAVTLKDAHGNLVEGGESLLSGDNVTVEGAVRSGGWSETAGVYTATWSAQMAGDSH
                     HATLTLPEWGGSKQSESYSIHSGAPVQANSAIRTDKSAYIAGEPLTVTVTLRDEFGNP
                     ALGLTSEVIESYIDNFAVGGATPDSLYWVEQNSGEYTIVWTAWVAEENLVASLKLKTW
                     AMEIKSSLYGIQPGAAAQTQSTIVTDKTKYIAGDSITVTVVLKDAQGNFITDGVAQLN
                     EENVQVRNADSIQGNNWVYNGDGKYQRQYMAHFAEANLNAQLKMAGWSDANYSKNYTI
                     NRGEVSMFRSQLRIHEVLVVAGADIPVSVLLADEFGNPVNDGLDLLTDDAVYLQNVEK
                     KHWSSWTFVGDGRYERTYMAYKEGENLNSYLHINGWYVGGQPSYTILPFVEVESLSVN
                     GAKFRAADGFPKTGFDGAKFTLILTHNMKNTDYNWTSGIQGIQVDSNGMVTLEFIINK
                     EVTITGTPKSNKGNKVTYKFSLQKWFIPQGIIQESWSEMNSYCIGNGYILPSSTDLVG
                     SSTSGAVPRKVGSLWGEYGNLTSYDGIFRAEHYWLDSGMIFYPGDGHLSIASRSSPLC
                     MKTF"
     gene            complement(12893..>13162)
                     /locus_tag="RHW33_003910"
     CDS             complement(12893..>13162)
                     /locus_tag="RHW33_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1647366.1"
                     /translation="VNSLYTKFLIYGASIKNDIMKLLANINTNKINGVILPLIYTVYG
                     KKEYEKRWQQLIKENDLWSNVLLHDPRLLSVKNEFNTIGVMRSSA"
BASE COUNT         3178 a         3090 c         3701 g         3193 t
ORIGIN      
        1 ttaaagaata tccattaatg tgcaattgaa atgtgataat tatcactgaa ccgttgttgt
       61 catttgtttt acaaaagcaa gggattaaat ctcctccaat ggacaaaaaa atgaataatg
      121 acaataccga ttacgtgagt aatgaatcag ggacgctttc gcgattattt aaactacctc
      181 agcatgggac caccgtccgc acagaattga ttgcggggat gaccactttt ttaaccatgg
      241 tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc acaaatggac ccgaaagtgg
      301 tgtttgttac cacctgtttg attgccggta tcggcagcat tgcgatgggg atatttgcta
      361 acttacctgt ggcgctggct ccggcaatgg ggctgaacgc cttctttgcc ttcgtggtcg
      421 tgggggcgat gggtatctcc tggcagaccg ggatgggcgc gatattctgg ggtgcaattg
      481 ggctattttt gctcacgctg tttcgtatcc ggtactggat gatctccaac attccattaa
      541 gtttacgtat tggtatcacc agcggaatcg gattatttat cgccttaatg ggattaaaaa
      601 acactggcgt tattgtcgcc aataaagaca ctctggtgat gattggcgat ttaagttctc
      661 acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca tcacgtcatt
      721 ttcatgccgc ggtgctggtt tccatcgtcg tcacttcctg ctgcggatta tttttcggtg
      781 atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc attggtgaag
      841 tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc tttatgctga
      901 tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagct ggcttaatag
      961 atagtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc gtcagttcgg
     1021 tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt acttctggtg
     1081 tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg ttcctgttgg
     1141 ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgct ggagcgttaa
     1201 tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat gattttaccg
     1261 aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg atcaccgaag
     1321 ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg cgctggcgcg
     1381 atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt attctggtgg
     1441 attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg agaactcatg
     1501 atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg ggtgctgaca
     1561 tccggggcca gaaaagcgat tcgccgtctc agcgatcggc tgaacatcga tgtcgtaccc
     1621 gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt acgttatttg
     1681 catcgtccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat ctggcaaatt
     1741 gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt acaaaaagcc
     1801 cgccttgccg cgccgattac cgatgctcag gtcaggctgg cgctgggttt tctgcgcgaa
     1861 actgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc attctttcag
     1921 cctgccgagg gcgttcattg gctgcattaa aaccgcttcg tcttatcagc ctacagaccg
     1981 cgcctgacct gtcgagtgga taaggcgttc acgccgcgtc cggtatcgga actgacagta
     2041 ggtgacgcat tatctacgtt ctatttacag cagcacatcc agcccgtgtt tttgcacaat
     2101 gctgagcagt tttaatgaaa tgccattggg gcgtttagcg ccactttccc atttttgtac
     2161 agttgaaacg ctggtattga gatagtgagc gaagacgggc tgactcactt ttaatttttc
     2221 ccgcagtgct tttatctcaa caggttgtag gtcttgcacg ctggtcaggc aggctttgtc
     2281 aaaagtacgc atcgtttcgg cagaaatagc atcgacgctg gacaatccag ccgctgcgct
     2341 gtgaatagcc tcaaatgcag gacttttaaa tttagttttt gcagtcatag cagatctctg
     2401 tcagttcttt agtgttaacc attgctgtta gctgggcttt cgtcaggaag gcataatgct
     2461 ttgcaagttc acgaaaccca gccagttcct gactattgat gttcgacata tcctgttttg
     2521 catataaaaa cgtgtaaaac caattgcttc ccccttttgc cagaataatt gcacgctcac
     2581 gattttggtg aaggcgcttt ttaaaaacgc caccaccaag tgcatcagct ttaccttgct
     2641 ccgtttctaa aatggcccgg cacaactctt catctgttat tgcatgagac ctggcttccc
     2701 ttgcaaacca tttggtttta aacacgcgca tatcgttgag gtcctgttat taaagtatat
     2761 caccaagtgc aatactttat cccttattcc caacccaatc gcgaaaatgc atatcagctt
     2821 gccctatgat ttttatttct tcgagcagcc tcgaactttt cccacaacac cgtaaaagtt
     2881 gcaaaaacgt taaatgcgtc acacatttca atgttaaagt ggtcggcttt tcccctgaaa
     2941 catgccacgg gtaacaccat gagtgaattt attgccgaaa accgcggcgc ggatgccatc
     3001 acccgaccga actggtcagc cgttttctcg gtggcgtttt gtgtcgcctg tctgattatc
     3061 gttgagtttt tgcccgtcag tttgttgacg ccaatggccc aggatttagg catttcggaa
     3121 ggggttgccg ggcaatcggt gaccgtgacc gcctttgtgg caatgtttgc cagtttgttt
     3181 attacccaga caattcaggc cactgaccgc cgctacgttg ttattttatt tgccgttttg
     3241 ctgacgctct cctgtttgct ggtttccttt gctaactcat tcagtttgct tttaatcggt
     3301 cgtgcctgtc tggggctggc gctgggcggg ttctgggcga tgtcggcgtc gctgaccatg
     3361 cgtctggtac cgccgcgtac ggtgccgaag gcgctgtcgg tgatcttcgg cgcggtttct
     3421 attgcgctgg tgattgccgc gccgttgggc agttttttag gcgagcttat cggttggcgc
     3481 aatgtcttta atgcggcggc ggtgatgggc gtgctgtgta ttttctggat tatcaaatca
     3541 ctgccttcac tgccaggcga accctcgcat cagaaacaaa atactttccg cttattacaa
     3601 cgtccgggtg tgatggcagg gatgatcgcc atcttcatgt ctttcgccgg gcagtttgct
     3661 ttcttcacgt atattcgccc ggtgtatatg aacctggcgg gattcggcgt ggatggctta
     3721 acgctggtgc tgttgagttt tggtatcgcc agttttattg gtacgtcgct ttcgtcgttc
     3781 attcttaaac gttcggtaaa actggcctta gcaggcgcgc cgttaatact ggctgtgagt
     3841 gcgttggtgc tgacgttgtg gggaagcgat aaaatcgttg ctaccggcgt ggcgattatc
     3901 tgggggctaa cttttgcatt ggttcccgtc ggctggtcaa cgtggatcac ccgctcactg
     3961 gccgatcagg cagaaaaagc cgggtctatt caggtggcgg ttattcagct tgctaatacc
     4021 tgtggcgcgg caatcggcgg ttatgcgctg gataatattg gtctgacttc gccgttgatg
     4081 ttgtccggca cattgatgtt gctgacggcg ttattggtca ccgcaaaggt gaaaatgaag
     4141 taacgtattt acaggagggt tattgctctg gcgggaaagc tctcctgttc ttccccctga
     4201 taacccttga tcaggtattg ttcttatctt gttgttattt ccattattcc gcctgtattc
     4261 atcatttgct aatcatgaat catcattttc cctcatgtta aagaaaggtc atttaatctg
     4321 aacgcatagc gacagggatg acaaatggca gggaaagcgc atgggaatgg cgatcgtcgg
     4381 ggcgataaca ccatatgtgg attgggggat cgtttacgtc gtctgaccgc cggtatttgc
     4441 ctgataacac aaactatatt ccctgttatg gccgctgccc ctacgcatat taatcatgcg
     4501 cgttcggata ctgccacgtc cctgatcctg ccgaacgtta aaactattcc atataccctg
     4561 ggggcattgg aatcgccatc aacggtcgcg gcacgctttg gtatcaccgt tgatgagtta
     4621 cgtcgcctga accagtttcg tacctttgcg cggggctttg acaatgtgcg tcagggcgat
     4681 gaaatagatg tgcccctcat taacagtaat agccctgaag cgcgcaacct gaaagcaatg
     4741 cagatggagc gcgacggcaa agatccgcaa atgcaggtcg cggaagtggc gcagcaaagc
     4801 ggaacacttt tagctcgcga tatggacagc gaacaggcgg cgagtatggc gcgtggctgg
     4861 gtggcttctt ctgcatctgc gcaggcaacg gactggctca gtcgttgggg aactgcccgg
     4921 gtatctcttg gtgtggatga agatttcagc cttaaaagct cttcattcga atttctccat
     4981 ccctggtacg aaacgccgga taacctggtc ttcagccagc acacacttca tcgtacggat
     5041 gatcgcactc aaaccaacca cggtatcggc tggcgttatt tcaccccgag ctggatgtct
     5101 ggcgtcaata tgtttatcga tcatgacctg acccgctatc acacccgaac cgggatgggc
     5161 gttgaatact ggcgcgatta cctgaaactg agcggcaacg gttatctgcg cctgagtaac
     5221 tggcgtagtg caccggaact cgaccatgac tatgaagcac gtccggcgaa cgggtgggat
     5281 cttcgcgccg aaggctggtt accggcctgg ccgcagctgg gcggcaagct ggtctatgaa
     5341 caatattatg gcgatgaagt ggcgctgttt ggtaaagacg agcgacaaaa tgatcctcac
     5401 gcgattaccg caggtttgag ttacacccct gttcctctga tctcctttag tgccgagcaa
     5461 cggcagggca aacagggaga aaacgacacc cgaattggta tggagttgac gttgcaacct
     5521 ggtcattcac tgcaaaaaca gctcgatccg gctgaagttg cagcacgacg cagccttgtg
     5581 ggcagtcgct atgacctggt cgatcgtaat aacaatattg tcctcgaata tcgcaagaaa
     5641 gagctggtgc ggctgacgct gaccgatccg ttgaaaggca agccgggtga ggtgaaatcg
     5701 ctggtttctt ccttacagac caaatatgcg ctgaaaggtt atgacattga agccgcctcc
     5761 ctgcaatcag cgggcggaaa agtagctgtt tccggcaagg atattcaggt cactattcct
     5821 ccctaccgat tcacggcaat gcctgaaacc gacaacactt acccgatagc ggttacagcg
     5881 gaagattcga aaggaaattt ctcccggcgt gaagaaagta tggtggtcgt ggaaaaaccc
     5941 acattaagcc tcgccgattc aacgctttct gtcgatctgc aaatcctgct tgctgatgga
     6001 aaatcaacct caacgctgac ctataccgcg cgagattcca gcggcaagcc aatcccaggt
     6061 atgacgttaa aaacacaggc gaaaggtttg caggatttcg ccttaagcga atggaaagac
     6121 aacggtaacg gaacctatac ccaaatagtt accgccggga agacgtctgg cgcgttatcg
     6181 ctgatgccgc aatttaacgg cgacgatatc gctaagacgc cagcgctgat tgctatcgtg
     6241 gcgaatacgg catcccgcgc tgactccacg attgagacag accaggataa ttacgtcgcg
     6301 ggtaagccga tagtcgttaa agtgaccctg agagatgata acggcaacgg ggttaccgga
     6361 cgaaaagaac ttctcaaaca aaccgttaaa gtagacaaca cgaaggctga tgccgttagt
     6421 gcctggacgg aagaaagtga gggaatttac aaagcaagct atacggccca tctcattggc
     6481 gacaaattaa cggcccagct gacgatgccc ggctggcaaa ccaaacattc ggatgcgttc
     6541 agtattgcgg gtgataaaga taccgccaaa atcgctgcga tgcagatcac cgcgaataac
     6601 gcagttgcca gacgcgatca caataccgtc gctgtgacgg tcagggatgt gcatcaaaat
     6661 ttactgcagg gacaaaatgt taccttcacg gtcgtgaatg gtgcggcggt ttttgccgat
     6721 ccgaatggcg gtattgtgac gacagataaa gacgggatag ccagcgttaa ccttgccagt
     6781 gaccaggcgg taaacagtct tatcaaggcc gagatcaatg gcagtagtca gtctgtggaa
     6841 gtgagcttta tcactggcga tatctcgcag ctgacctcca ccatcaagac ggacgatgtg
     6901 tcatacactg cgggtggcaa gataaaagtg tccgtcaccc ttatggatga gcagaaaaac
     6961 ctggtgaaag ggatggcttc gctgctggcg ggtagcggtg tggtcgaggt gagcggtaca
     7021 gataaaaatg aaacgggtaa ctggagcgaa gagtctgacg gcgtttacac caccacccga
     7081 accgcgaaga ttgctggcga tcgccattac gccacgctga aactatcgac atggagtagc
     7141 gcgcagcaat ccgatgcgta tgccatccgc gaaagcggag ctgtgctggc ttattcctcc
     7201 atcgttaccg acaaaacgac ctacaccgcg ggcggcgtga taaaagttac ggtaacactc
     7261 aaggatagct atgaaaacct ggtcgggggg caacgagacg ctatcaacca ggctattcag
     7321 ctgccaaata caaaagcgga aagcatcgcc tggaatgaag atcaaaaagg aatttatacc
     7381 gcaacgtata ccgccttact ccctggcacc ggtttaaaag cacagctaca gatgtccgac
     7441 tgggcgagcg ctctgacttc aaatgattac agtattagtg gcgatgccgc cagcgcacaa
     7501 attgtcgcga tgcaagtgac aacgggtaac ccggatgtgc tggcgaatgg cagcgatcga
     7561 catacggtga atgtccgggt ggaagatcag tttggcaatg tgctgccgga gcagaccgtc
     7621 acctttacgg tgacgaaggg ggcggctgta tttgccaatg cagggcagag cgcagatatc
     7681 aggacggatg cgcatggtat ggcagaggtt gatctgagca gtaccgtagc ggatgccagc
     7741 actgttgagg ctaaggtcaa tcaaagcagc gatagtaaaa cggtgaactt tgttgctgat
     7801 gtcagcacgg cgcagatcgc tgatctggtg gtcattaagg atggctcagt ggcagacggt
     7861 tcgacggcga acacgctgcg ggtgagggtg acggatgcgt tcggtaatgc gctggacggg
     7921 cagacggtca gcgtgttggc ggacaacggc gcaacggtgt ctccgacggt catcacaggc
     7981 ccggacggca cggtggagat ctctgtcacc agccagactg ccggaatcag tgcggtcacc
     8041 gccagcatca acagcagcaa cgcgagcagg aacgtgacgt ttattgcgga tgtcaggacg
     8101 gcgcagatcg ctgatctggt ggtcattaag gatggttcag aggcggacgg tgcgacggcg
     8161 aacacgctgc gtgcgagggt aaccgatgca ttcggaaatg cgcttgccgg gcagacggtc
     8221 agcgtgttgg cggataacgg cgcaacggtc gctccgacgg tcatcacagg gccggacggc
     8281 acggtggaaa tctcagtcac gagccagacg gcgggcacca gtgcggtcac tgcaaccatc
     8341 aacagcagca gtcagagtcg gaacgtgaca tttatcgccg atatcaggac ggcgaagatc
     8401 gcggatctgg tggtcattaa ggacggctca gtggcagacg gtgcgacggc gaatacgttg
     8461 cgggcgaggg tgaccgatgc gttcgggaat gcacttgccg ggcagacggt cagtgtgaca
     8521 gcaggcaacg gcgcaacggt tgccccgacg gtgattacag agccggacgg gacggtggaa
     8581 atcagtgtca ccagccagac ggcggggatc agtactgtca ctgccagcat caatagcagc
     8641 agcctgagcc gggatgtgac gtttattgcg gatgtcagga cggcgcagat cgcggagctg
     8701 gtggtcatta aggatggttc agaggcggac ggttcgacgg cgaacacgct gcgtgcgagg
     8761 gtaaccgatg cattcggtaa cgcgcttgcc gggcagacgg tcagcgtgtt ggcggacaac
     8821 ggcgcaacga ctgccccgac ggttatcacc gagccggacg gtaaggtgga gatctctgtt
     8881 accagtcaga cggcgggagt cagtgcagtc actgccagca tcaacaacag caccctgagc
     8941 cagaacgtga tgtttatcgc cgatatcagg acggcgcaga tcgcggagct ggtggttatt
     9001 aaggatggtt cagaggcaga cggtgcgacg gcgaacacgc tgcaggtgaa ggtcaccgat
     9061 gtgttcggga atgcgcttgc cgggcagacg gtcagcgtgt tggcggacaa cggcgcaacg
     9121 gtcactccga cggtgaccac agagccggac gggacggtgg aaatcagtgt caccagccag
     9181 acggcgggga tcagtacagt cactgcaacc atcaacaaca gcaccctgag tcagaacgtg
     9241 acatttatcg ccgatgtcag gacggcgaag atcgctgatc tggtggtcat taaggacggc
     9301 tcagtggcag acggtgcgac ggcgaatacg ctgcaggtga cggtcaccga tgcgttcggt
     9361 aatacgctta ccgggcagac ggtcagcgtg ttggcaggca acggcggaac ggttgcccca
     9421 acggtgatta cagagccgga cgggacggtg gagatctctg tcaccagcca gacggcggga
     9481 gtcagtgcgg tcaccgccag tatcaacaac agcagcctga gccagagcgt gatgtttatt
     9541 gccgatatca ggacggcgca gatcgcggat ctggtggtca ttaaggatgg ttcagaggcg
     9601 gacggttcga cggcgaacac gctgcgggcg agggtgaccg atgcgttcgg gaatgcgctt
     9661 gccgggcaga cggtcagcgt gttggcggac aacggtgcag cggttgcttc gacgatgacc
     9721 acaaagccgg acggcacggt ggagatcagt gtcaccagcc agacggcggg caccagtgcg
     9781 gtcaccgcca gcatcaacag cagcagtcaa agccgggatg tgacgtttat cgccgatatc
     9841 aggacggcgc agatcgcgga cctggtggtc attaaggacg gctcagtggc tgacggcgca
     9901 atggcgaata tgctgcgggc gagggtcacc gatgcgttcg gtaatgcact tgccgggcag
     9961 acggtcagcg tgttcgcagg caacggtgcc acgactgctc cgacggtcac cacgcagccg
    10021 gacggcacgg tggagatctc tgtcaccagc cagacggcgg gagtcagtgc ggtcactgcc
    10081 agcattaaca gcagcagcca gagccgggat gtgacgttta tcgccgatgt cagaacggcg
    10141 aagatcgctg atctggtggt cattaaggac ggctcagtgg ctgacggcgc aatggcgaac
    10201 atgctgcagg taaaggttac cgatgctaac ggcaatgtgc ttgccgggca gacggtcagc
    10261 atgatggcag gcaacggtgc aacggttgcc ccgacggtga ttacagagcc ggacggtacg
    10321 gtggagatcc ctgtcaccag ccagacggcg ggagccagtg cggtcaccgc cagtatcaac
    10381 agcagcaacg cgagccggaa cgtgacgttt gttgccgatg tcaggacggc gaagatcgcg
    10441 gatctggtgg tcattaagga cggctcagtg gcagacggcg caatggcgaa cacgctgcag
    10501 gtgaaggtca ccgatgcgtt cggcaatgcg cttggcggac agacggtcag cgtgacggca
    10561 ggcaacagcg caacggtcac tccgacggtc accacgcagt cggacggtac ggtggagttc
    10621 agtgtcacca gtcagacggc gggagttagc gcagtcacag caaccatcaa caatcatagc
    10681 ctgagccaga acgtcacctt tgtaccgggt gacgcatcgc agctcacttc aaccgttgaa
    10741 acaaataagt ctaactacac ggttggagag accatcacca tcacggtaac gctccgggat
    10801 gcgttcgata atctggtaac cggtgcagct tcacagttag ccgctaatag tgtgctgacg
    10861 gtggatggca ccgacccgtc agaaacggga agctgggttg aatcaggtgg cgtgtatacc
    10921 acaacccgaa tggcgacgat tgccggtacc gatcagcacg ctaacctgca actgcagacg
    10981 tggagtgatg gtgtaacgtc agatcgctat gacatacagt ccggttctcc ggcgcaagcc
    11041 acatcaacta tcgccacaga taaaaacgca tacatcgctg gcgagactat aaccgtcgcc
    11101 gtgacgctga aagacgcgca cggtaacctg gttgaagggg gcgagtcctt actgtctggt
    11161 gataacgtaa cagtagaagg agccgtacgt tcaggaggat ggtctgaaac tgccggtgtt
    11221 tatactgcta catggtcggc gcaaatggcg ggagactctc accacgcgac gctgacgttg
    11281 cctgagtggg gcggcagtaa acaatcagag agttattcca tccacagtgg tgctccggtg
    11341 caggcaaatt ctgctatcag gacagataaa tcggcatata tcgccgggga gccattaacc
    11401 gtcacggtaa cgttgcggga tgagtttggt aatcctgctt tagggctgac atcagaagtc
    11461 atcgagtcat atatcgataa tttcgcggta ggcggcgcaa ctcccgattc tctgtattgg
    11521 gtcgagcaga atagtggtga atataccatt gtctggacag catgggttgc cgaggaaaat
    11581 ctggttgcca gtctgaaatt aaaaacatgg gcaatggaga ttaagtcctc attatacgga
    11641 atacaaccag gtgcggcggc acaaactcaa tccacgattg tcacggacaa aacgaaatat
    11701 atcgctgggg atagcataac ggtgaccgtt gttcttaaag atgcccaggg taactttatt
    11761 actgatggtg tcgcgcagct taatgaggaa aacgtacaag tcaggaacgc tgattctatt
    11821 caaggcaata attgggttta caacggcgat ggtaaatatc aaagacagta tatggcgcat
    11881 tttgcggaag cgaacttgaa tgcgcaactg aagatggctg gttggagcga tgccaattat
    11941 tcaaaaaact acactattaa cagaggtgaa gtaagtatgt ttcgttcaca gcttcgtata
    12001 cacgaagttt tagtggtagc tggggcggat atacctgtca gtgttttatt agctgatgaa
    12061 tttggtaatc ctgtgaatga cggtctggat ttgctgacag atgatgcagt gtacttacaa
    12121 aatgtggaga agaaacattg gtccagttgg acatttgtag gtgatggcag atatgagcgt
    12181 acctatatgg cttacaaaga aggagaaaac ttaaactctt atctacatat taatggttgg
    12241 tatgtaggcg ggcaaccgtc ttataccatc cttccttttg tggaagtcga gtcgcttagt
    12301 gtcaatggtg caaaattcag ggcggcagat ggttttccca aaacagggtt tgatggtgct
    12361 aaatttacac tgatattaac ccacaatatg aaaaatacgg attataattg gaccagtggc
    12421 atacagggta ttcaggtcga tagtaatggt atggtgactt tagaattcat tatcaataaa
    12481 gaggttacta ttacaggaac gccgaaaagc aataaaggga ataaagttac atataaattc
    12541 tcactgcaga aatggtttat acctcaaggg ataattcagg aatcatggag tgagatgaat
    12601 tcatattgta ttggtaatgg ttatatatta ccgtctagta cggatttagt tgggagttca
    12661 actagcggtg ctgtcccgcg aaaagtaggt tcgttatggg gtgaatatgg taatctgact
    12721 tcgtatgacg gaatatttcg cgctgaacat tattggctcg acagcggtat gatattttat
    12781 ccaggagatg gtcatctctc gattgcgtca cgttcatccc cactgtgtat gaaaacgttc
    12841 tgatattatt tatgttattg ccctgcatca gcagggcaat ttatcttcgc tcttacgcag
    12901 aactccgcat cacaccaata gtattaaatt catttttcac actgagcaaa cgggggtcat
    12961 gaagcaacac atttgaccat aaatcattct ctttaattaa ttgttgccag cgcttttcat
    13021 attctttttt accataaaca gtgtaaatta acggcaaaat aacaccattg attttgttgg
    13081 tgttaatatt tgccagtaac ttcataatgt cattttttat agatgcgcca tagatcagaa
    13141 acttcgtata aagcgagtta ac
//
LOCUS       DAPGRN010000126        13159 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPGRN010000126 DAPGRN010000000
VERSION     DAPGRN010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.128"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            246..1166
                     /gene="cbpA"
                     /locus_tag="RHW33_003911"
     CDS             246..1166
                     /gene="cbpA"
                     /locus_tag="RHW33_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV1647367.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFSRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1166..1471
                     /gene="cbpM"
                     /locus_tag="RHW33_003912"
     CDS             1166..1471
                     /gene="cbpM"
                     /locus_tag="RHW33_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV1647368.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1623..2222)
                     /gene="torD"
                     /locus_tag="RHW33_003913"
     CDS             complement(1623..2222)
                     /gene="torD"
                     /locus_tag="RHW33_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV1647369.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVDELENRVATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFASRCRQYDSFGFYAALSQLLLVLVEGDHQNR"
     gene            complement(2219..4765)
                     /gene="torA"
                     /locus_tag="RHW33_003914"
     CDS             complement(2219..4765)
                     /gene="torA"
                     /locus_tag="RHW33_003914"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV1647370.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQTYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKL"
     gene            complement(4765..5937)
                     /gene="torC"
                     /locus_tag="RHW33_003915"
     CDS             complement(4765..5937)
                     /gene="torC"
                     /locus_tag="RHW33_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV1647371.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6067..6759
                     /gene="torR"
                     /locus_tag="RHW33_003916"
     CDS             6067..6759
                     /gene="torR"
                     /locus_tag="RHW33_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV1647372.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPYTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6732..7760)
                     /gene="torT"
                     /locus_tag="RHW33_003917"
     CDS             complement(6732..7760)
                     /gene="torT"
                     /locus_tag="RHW33_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV1647373.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            7843..10587
                     /gene="torS"
                     /locus_tag="RHW33_003918"
     CDS             7843..10587
                     /gene="torS"
                     /locus_tag="RHW33_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV1647374.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQRLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLSLF
                     QQDSEISNHLQTLAQNNIAQFALFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELNAISTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFCGIIPKPVPREVLGQLLAHYL
                     QLQVNNDLPLDVSQLNEDAQLMGTEKIHEWLALFKQHALPLLDEIDISRASQDSEKIK
                     RAAHQLKSSCSSLGMRSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLNKKDLNAI
                     "
     gene            10659..11732
                     /locus_tag="RHW33_003919"
     CDS             10659..11732
                     /locus_tag="RHW33_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV1647375.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFLARPGGIEGWLPIAGLMNLKYSLATGQVPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGALGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGMVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLILGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(11781..11954)
                     /locus_tag="RHW33_003920"
     CDS             complement(11781..11954)
                     /locus_tag="RHW33_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV1647376.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(11944..12174)
                     /gene="ymcE"
                     /locus_tag="RHW33_003921"
     CDS             complement(11944..12174)
                     /gene="ymcE"
                     /locus_tag="RHW33_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV1647377.1"
                     /translation="MHRWISQNNIRLPRGAFFISVLFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            complement(12149..12337)
                     /locus_tag="RHW33_003922"
     CDS             complement(12149..12337)
                     /locus_tag="RHW33_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV1647378.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            complement(12348..12560)
                     /gene="cspG"
                     /locus_tag="RHW33_003923"
     CDS             complement(12348..12560)
                     /gene="cspG"
                     /locus_tag="RHW33_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV1647379.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            12846..13058
                     /gene="cspH"
                     /locus_tag="RHW33_003924"
     CDS             12846..13058
                     /gene="cspH"
                     /locus_tag="RHW33_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV1647380.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRYAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         3172 a         3441 c         3344 g         3202 t
ORIGIN      
        1 aaatttaata aataatgacg cactagtaaa acttaaagtg cctgtttcaa ctatcaaaaa
       61 tcgctcaccc tttttcacct gtttaaaata tgttcagcgc cccaccttga ggcgacctcc
      121 tctccgcgat gatttcaata acatattctg tgttggcata tgaaattttg aggattaccc
      181 tacacttata ggagttacct tacaggggtt ccttcaattt gtgttgattt acgcgagata
      241 acgctatgga attaaaggat tattacgcca tcatgggcgt gaaaccgacg gacgatctca
      301 agacaatcaa gaccgcctat cgtcgacttg cccgcaaata ccatcctgat gtcagcaaag
      361 aaccggatgc cgaagcccgc ttcaaagagg tcgctgaagc ctgggaagtg ttaagtgatg
      421 aacaacgtcg cgctgagtat gatcagatgt ggcaacatcg caacgatccg caatttagcc
      481 gtcagttcca gcatggcgac ggtcagagtt ttaacgccga agattttgac gatatcttct
      541 cgtcaatttt cggtcagcat gcccgccaga gccgtcaacg ccccgccaca cgcggccacg
      601 atattgaaat cgaagtggcg gtattcctcg aagaaacgct tactgagcat aagcgtacca
      661 tcagctataa cctgccggtt tataacgcct ttggcatgat cgaacaggaa atcccgaaaa
      721 cgctgaatgt gaagatcccg gcaggcgtcg gcaatggtca acgcatccgc ctgaaaggcc
      781 aggggacgcc gggcgaaaac ggcggtccaa atggcgattt gtggctggtg attcatattg
      841 cgccacatcc gctgtttgat attgtcggcc aggatctgga aattgtggtg ccggttagcc
      901 cgtgggaagc ggcgctgggt gctaaagtca ccgttccaac actgaaagaa agcattttgc
      961 tgactatccc gcctggcagc caggccgggc aacgattgcg cgttaaaggc aaaggtctgg
     1021 tgagcaaaaa acagaccggc gatctgtatg cggtactgaa aatcgtgatg ccgccgaaac
     1081 cggatgaaaa cactgccgcg ctgtggcagc aactggcaga cgcccagtcg tcttttgatc
     1141 cacgtaaaga ttgggggaaa gcataatggc taatgttacg gtgactttta ctattaccga
     1201 attttgcctg cataccggca tctctgaaga ggaattgaat gaaattgtcg gtttgggggt
     1261 ggttgaaccg cgtgagatcc aggaaacaac ctgggtattt gacgaccatg ccgccattgt
     1321 ggtgcaacgc gcggtgcgcc tgcgtcatga actggctctg gactggccgg ggatcgcggt
     1381 ggcgctgacg ttaatggatg atattgcgca cctgaagcag gaaaaccgcc tgctgcgcca
     1441 gcggctttcc cggtttgtag ctcatccgtg atgtgtgttt gccggatgtg atgacgcatc
     1501 ttagcctgcc tgcaaagagt acttttcctg tgggccggat aaggcgttca cgccgcatcc
     1561 ggcggcggtg ccctaatgcc tgatgcgacg ctaaacgcgt cttatcaggc caacaaacga
     1621 cgttatctgt tttggtggtc gccctcaact aacaccagca ataactggct tagtgccgcg
     1681 taaaaaccaa agctatcata ctgacgacaa cgcgaagcaa actctggtaa ccattgccgc
     1741 agcgccgtga gtgttttttg ccgtaaactg tcgattcttc gcgcaggaac cgtcccctct
     1801 cccagcgaaa aatgcagatg actcaacaac tcgagataga tcgccagatg atctgccggt
     1861 tcgttgaaat tgccgctggt ttccatccct gcctcaacta acaagcgttt aatctcttgc
     1921 tcgtcctgtt tgtaggccga tgcatacggc agcgccgctt gtttgtcagt catcagaaac
     1981 aggccgcaaa agtcagcggc cagttccaga cgggcatcgt cacgtactgt cagcgtggca
     2041 acacggtttt ccagctcatc caccgccgca gtgagcggcg gttcgctttt cagcaacgaa
     2101 aaccattcag ccatctgcgc actggcgatt tgcgtcagtt gttcatcgtc cagttcacgg
     2161 gagaacaact gcgccagcca ggcgtaaaca caggcaatct gttgtgctgt cagcgtggtc
     2221 ataatttcac ctgcgacgcg ggaacatatt cgcactgcgc caccatctcc acggggccgt
     2281 taaacgccgt tacctgctcc actgttccgt tgtacttctc aatttccacc agcgtagtgt
     2341 gcgcactggt cgcctgcgcc agctgcgaag taccgatgtc gatggtcaac acgttggggt
     2401 taccgtattt gcacagcgca ccaggctcgc cgcctttatc tggatcgtac catgcccctt
     2461 cgtgaattcg cgccacgccg ggtgcatagc ggtcagaaac gactgcccct gccaacacct
     2521 gaccgcgagc gttaaagacg cgtaccacat caccgttacg aataccgcgc gcgctggcat
     2581 cctgcgggtt aatgaacact ggctctttac ccgctaccgt atattgctga cgcagagttt
     2641 ccgactcaca taactgcgag tgaagtcgga aatccggatg cacagattgc agatgcaacg
     2701 gatacgtctg cgagccaggc ccaccgtggg agcgttcgat tttctcaaac cacatcggat
     2761 gcccctgaca atcgtcgtag ttcatatcgg cgatggtttt cgaatagatc tcaatcaggc
     2821 cactcggcgt gcccagcggt tcaagatccg gatcttcgcg gaatgcctgg tggcgaacaa
     2881 acatctgcgg atggtcaaac tcgacgtact ctttgttatt ccagaagtca tcaaacgctg
     2941 gcagatgaac gccgcgtcct ttgccttgct gaacaccttc ctgccagatg cgtttcagcc
     3001 agcccatttc gtccagccct tcggtaaagg cttcttcgcg attaaagcgg cggcacagct
     3061 cgcggaaaat atcaaagtcg ttgcgcgcct cgaactgcgg cggcacgacc tgtttcatgg
     3121 cgataatgcc acggttggag tggttgccgt actggtcgag atcgttacgc tcaaactgcg
     3181 tggtcgcagg cagcacgata tcggcaaagc ggcaggttga ggtccactgg ttatctatgg
     3241 cgataaccgt ttccagcttg cgccagcctt caataatgcg gttgatctgc tgatggcgat
     3301 ggaatgggtt agttccggca aaaatacaca ttttcagcgg cggcagtttt accgatttac
     3361 cgttccagtt gatcactttc cccggttcga ggatcgcatc gataaaacgg gcaatcggaa
     3421 tggtgctgct gtagcctttg taatcactgt tgtcgtgaac aggcggaatc gacgtagagc
     3481 cggagaaacc actcagaata acgcctttac gccccggcgt gcctgcgccg ttatagtgcc
     3541 agccaaaacc aaagccacca cctggcaggc caatttgccc cagcatcgcc gccagaacca
     3601 caatcatcca cgcccactgt tcaccgtgct gcatacgctg tacgcaccag ccagcaataa
     3661 tctgcgttct gttcgccgcc atctgccgcg ccagcccacg aatggtttcg gcatcaatgc
     3721 cggtcagttt ttcagcccat gcggcatctt tcggctgacc gtctttctcg cccagcagat
     3781 acggcaggaa ctgctcaaaa cccacacagt agttagcgag gaagtttttg tcgtacaggt
     3841 tttcactgta cagcgtatgc gccagcgcca gttgcagcgg cacgtcagtt tgcgggttaa
     3901 ccgcaatgtg cttcacatgc tcgcgcccca gatactcatg ggtggatgta acaaccggat
     3961 cgatgctaat gacctcaatt tcaccggcgg cgactttcgc tttcaactgc gcgtaatatt
     4021 cataaacatc gtgatccggg caccaccagt tcgcttgctg gtttttcagc aaatcggagc
     4081 cccacagcac aatggttttg ctgttctgca ataccagcgg ccaggaggtt tgctgttcat
     4141 acacttccat cgaaccgact acgcgcggca ggatcacctg cgcagcacca gtagagtaat
     4201 ctccgcccgt accaacgcta ttaccatgca aggcaatagc tttcgccagc atccccgaag
     4261 cgttatggaa catccccgtc gattgccaac cactggcggt cagtaaggca ctcggcccgt
     4321 gagttttctg tacgcgttcc agttcttcat agaacatgtc gagggcttca tcccagctca
     4381 cacgcacaaa acggttatca ccgcgctggg aggtatcgct gagatggcgc ttacgcagcc
     4441 agtccacgcg taccatcgga taacgaatac gcgccgcgtt gtgtacgtgg tccggcaatc
     4501 cggcgatcat tttcgacgga tatttatcca gttcgaacgg ttttgccgcc acaaagcgac
     4561 catccttcac cgtcgcgcgg atagcccccc agtgcgaccc ggtaagaatg ccctctttcg
     4621 agataacagc ctcggtcgcc gcttgcgccg cagtcgcacg gcgcggcgtt aacaatgacg
     4681 gccccagcat cccggcgacg gttaagccgc cgagttgtgc cagaaaacgc cgacgtgatg
     4741 cctgaaagag atcgttattg ttcattattt ttcttccttc ttatcgccgt gagccttacc
     4801 tgcggtgtca gacgcattca tttgcagata tttcaacaag gtgcgttctt cacgtttatc
     4861 gaggctggta aagccaatca tgccgttgag cgtgccaatc cagccgttag cgtcaaagtg
     4921 agagatttcc ggtgcgccgt ggcactggtt acaggtgccg ttgtacaacg aatccgcata
     4981 agcccagatc ggtttgatat cgttcaccat gtcacctttc ttcatccacg cggtggcttg
     5041 caacttgctc cactcggtat tggtgtcggc aacggtggtt ttctccagcg tttttacctg
     5101 ctgctgcaca tcaccacgaa tcgaggcaac aaagatgcgt ttacctggga attgggtgag
     5161 tacacgctga cgtccggcgc tttctgtcca gccggtaatt tcaatttgca gccagtcgcc
     5221 gtcacgttta aggactttca cttccgaagc aggcagcagt gaaccagacg cttctttatc
     5281 gcctttcgcc gcataaattg gcttaatatc aatagagtac agcgtgtcac cactgtcatt
     5341 agcactggcg cgcagctcat cgaactgctt acggaagccg ctactcatat ccggtaactg
     5401 gtgggcaata cctttatgac agtcgatgca ggattgatta tctttcgctg ccaccttcat
     5461 ctgacgtgcc gcttcaggat gctgcttcgc atgatccatc gcgtcgtagt tatggcagga
     5521 gcggcaggtt gccgagttgt tttctttcat tcgtgcccat tcacgctcgg caagttccgc
     5581 gcgtttggct tcgaattttt caggtgtatc aatggagtgg gcaataaagg tctggtagat
     5641 atcgttgctc gcttccagtt tgcgcttcac catgcctgga atatccggcg ggatatgaca
     5701 gtcatggcat tcagctcgca cgccggaggc gttctggaaa tgcaccgact gtttatattc
     5761 ttcatacacc ggttgcatac tgtggcaact gacacaaaat tcggttgtgc tggtgacttt
     5821 gatcccaacg tgtggcaata caatcagcgc aatgccaatc acaatcccaa tagcgaccag
     5881 cgccagtacc gaccaacgag cactgggtcg gcgtagcgcg ttccagagtt tccgcataat
     5941 agcccctgta aaattatggt ttagtgaagc gatcttaatg agcaaatatg aacagcggca
     6001 ctggtcagga tgaatggctt acggcagaat atgaacagat atgaacagaa tgagtaaaac
     6061 cctctgatgc cacatcacat tgttattgtt gaagatgagc cggttaccca ggcgcgatta
     6121 caatcctact tcactcagga ggggtatacc gtttccgtta cggcgagcgg tgccgggcta
     6181 cgggaaatta tgcagaatca gccggtggat ttaattctgc tggatatcaa cttgcccgat
     6241 gaaaatggcc tgatgttaac ccgcgccctg cgagaacgct caacggtggg gattattctg
     6301 gttaccggac gcagcgatcg gattgaccgt attgttgggc tggaaatggg cgcagacgat
     6361 tacgtcacca aaccgctgga actgcgcgaa ctggtagtac gggtaaaaaa tctgctctgg
     6421 cgaatcgacc tcgcgcgaca agctcaaccg tacactcagg acaactgcta tcgctttgcc
     6481 ggttattgcc tgaatgtgtc gcgccatacg ctggagcggg atggtgagcc gattaaactg
     6541 acccgcgcag agtatgaaat gttggtggca tttgtgacga atccgggcga aattctcagc
     6601 cgtgaacgtc tgctgcgtat gctctctgcg cgtcgggtgg aaaaccctga cctgcgcacc
     6661 gtcgatgtgt taattcgtcg tttacgtcat aaactcagcg cggatttact ggtgacgcaa
     6721 catggcgaag gttatttctt agccgctgat gtgtgctgat aaaaatagac cggacgaaat
     6781 ccccccggtg acagcgagcg gcggatatgt tctcggtcgg catttttcgg cgtcagaact
     6841 aaaatcggtg ggctgacatt atcagacacc gattgcccct gtaattgcct gatggcctgc
     6901 tcaaccgcca gttccccctg ccagaccatt tgatcgctgg cagccataat cactcttccc
     6961 cgcttcagcc cgcgatacac ctgatgtgaa agataaaacg acaccacggt aagcggcgtt
     7021 ttcaggttgc gcccttctcc catagccgcc tctgccgcaa tggccgttcc ggcaacgacg
     7081 tcgatttctg gatggcgttc cagcatctcc tgcaacaggt tacgctggat ttcaatatcg
     7141 ttatcaccaa gcgcaatatc aacaatacgc accgggcttc cggcaatggc tgcgcgaaaa
     7201 ccctcgacca tctctttact gcccccggcg ttatcgggtc cgggcatcaa cagcacattc
     7261 agtggcttac cgtgcgccca ttgcaccaga tatcgcccag gttgatagcc catctgaaac
     7321 cagggtacac caacacggct tttcaccagg ggatcatcaa gagcatttac cagttcgatc
     7381 actggcagac ttgctacctg cttttgcagg tcgggaaatg acgtcgtgct actaccgagc
     7441 aaaatggcct ctgcgcccca ctgtttacac tggtcgattt gtgcttgctg ggtagccaac
     7501 tggctgtagc cgcctgcctc cagcactttt aaatccacac cgtagcggcg agcagcctcc
     7561 tgcattccat agttcaacga taaccagtat gaatctttca ggctgggata aagcgcgcac
     7621 agtttccatg cgcgtttggc tttaagcggc gtggaggctt gcaccgtgaa atgctgcgca
     7681 tcatgccagc gcaacaggtt atcagccgaa aatgccggca acatgaaaag ggaaagaagt
     7741 aaaaatagca gtacgcgcat gatagcctca tcaataataa ggctttatgc tagatgcatt
     7801 ccgctttgcg actcaacatt tttcacctaa agtgcaccga ccgtgaattt aaccctgacc
     7861 cgaagactct ggatgggctt tgccctgatg gcgctgttaa ccctgaccag taccctggtg
     7921 ggatggtaca acctgcgctt tatcagccag gtggaaaaag acaacactca ggcattgatt
     7981 cctaccatga atatggcgcg ccagttgagc gaagccagcg cctgggaact tttcgccgcg
     8041 cagaacctga ccagtgccga taacgaaaag atgtggcagg cgcaggggcg aatgctcacc
     8101 gcacaaagcc tgaagattaa tgcgttgctg caagcgttac gggaacaagg ttttgatacc
     8161 accgctattg aacaacagga gcaggagatc tcccgttcgt tacgtcagca aggggaactg
     8221 gtggggcagc gtttgcaact gcgccagcaa caacagcgac tcagtcagca gatagtcgcc
     8281 gccgccgatg agatcgcacg cctggcgcaa ggtcaggcga ataatgcggc aacctccgcc
     8341 ggagcgaccc aggccgggat ttacgatttg atcgaacaag atcagcgtca ggctgctgaa
     8401 agtgcactcg atcggctgat tgatatcgat cttgagtatg ttaaccagat gaatgaactg
     8461 cgccttagcg ctctgcgggt gcagcaaatg gtgatgaatc tggggctgga gcagatccag
     8521 aaaaatgcac caacgctgga aaagcagctc aataatgcgg tgaaaattct gcaacgtcgg
     8581 caaatacgca ttgaagatcc gggagttcgt gcgcaggtcg caacaacgtt aactaccgtt
     8641 agccaatata gcgatctgtt gtcgctgttc cagcaggaca gtgaaatcag caatcacctg
     8701 caaactctcg cccaaaataa tatcgcccag ttcgcgctgt ttagtagcga agtcagtcag
     8761 ctggtcgaca ccattgagct gcgcaatcag catggactgg cgcatctgga aaaagccagt
     8821 gcacgcgggc aatacagcct gttattgctg gggatggttt cactttgcgc actgattctg
     8881 atcctctggc gcgtggttta tcgctcagtc acgcgtccac ttgccgaaca aacgcaggcg
     8941 ctgcaacggc tgctggacgg tgatattgac tcccctttcc cggaaaccgc tggcgtacgg
     9001 gagctggaca ccatcgggcg gctgatggat gcgtttcgca gcagtgttca tgcactgaat
     9061 cgccaccgcg aacagctggc ggcgcaggtc aaagcgcgta cagctgaatt gcaggaactg
     9121 gtgatagaac accgacaagc acgggcggaa gcagaaaaag ccagccaggc aaaatcggcg
     9181 tttctggcgg cgatgagcca tgagatccgc acaccgctgt acggtattct cggcaccgca
     9241 cagctactgg cagataaccc cgcacttaac gcccagcgtg atgatttgcg ggcaattacc
     9301 gactccggcg aatcgttgct gaccatcctc aacgatattc ttgattattc cgctatcgaa
     9361 gcaggtggca agaatgtttc ggtcagcgat gagccctttg aaccgcgccc gctgctggaa
     9421 agtaccctgc aattaatgag cggacgggtg aaaggtcgcc cgattcgcct ggcaacggca
     9481 attgccgatg atgtaccgag cgcgttaatg ggcgatccgc gacgtattcg tcaggttata
     9541 accaacctgt tgagcaacgc cctacgtttt actgacgaag ggcagatcgt tttacgtagc
     9601 cgcactgatg gcgagcaatg gctggtcgaa gtggaagaca gcggctgcgg tattgatccc
     9661 gcgaaactgg cagaaatctt ccagccattt gttcaggtaa gcggcaaacg cggcggcacc
     9721 gggctggggc tgactatcag tagccgtctg gcccaggcga tgggtggcga actaaacgcc
     9781 atcagcacgc cggaggttgg aagctgtttt tgtttacgct tgccgttacg tgttgccact
     9841 gcacccgtgc caaaaacagt caatcaggcg gtgcgtcttg acggtttacg tttgctgtta
     9901 attgaagata acccgctaac ccagcgaatt accgttgaga tgctgaacac cagtggtgcg
     9961 caggttgttg ctgttggcaa tgccgcgcag gctttagaga cactgcaaaa tagcgaaccg
    10021 tttgctgccg cactggtgga ttttgatctg ccggatgtcg acggaattac tcttgcccga
    10081 caacttgcac agcaatatcc gtcgctggtt ttgattggct ttagcgccca tgtcattgac
    10141 gaaacgctgc gccagcgtac cagttcacta ttttgcggga ttatccctaa accggtgccg
    10201 cgtgaagtgc tcggtcagtt actggcgcac tatctccaac tgcaagtcaa taacgatcta
    10261 ccacttgatg tatcgcaact caatgaagat gctcaattaa tggggacgga gaagatccac
    10321 gaatggctgg cattatttaa acaacatgcc ctgccgcttc tcgatgaaat cgacatttcc
    10381 cgcgccagcc aggacagcga gaaaataaag cgtgccgcgc atcagctaaa aagcagttgc
    10441 tcgagtctgg gaatgcgtag cgccagtcag ctatgcgcac aactggagca gcagccatta
    10501 tctgcccccc ttccacacga agaaattaca cgcagtgttg ccgctctgga agcatggttg
    10561 aataagaaag acctgaacgc gatttgaatc aatatcagcc agttaagaat aactagaata
    10621 gtggcatttg gtatgcaact taacggactg gcaaaatcat ggcagagaaa aaaagaaccc
    10681 gctggcagcg gcggccaggc acgacgggcg gcaaattacc gtggaatgac tggcgcaatg
    10741 ccacgacctg gcgtaaagcg acgcaattct tactattggc aatgaatatt tatattgcca
    10801 tcacgttctg gtattgggtg cgctattacg aaacggcagg tagtacgaca tttctcgcca
    10861 gaccgggagg tatcgaaggc tggctaccga ttgccggtct gatgaatctg aaatatagcc
    10921 ttgcaacagg tcaggtaccg tccgtccacg ccgccgcgat gctgttattg gtcgctttta
    10981 tcgtcatcag tctattactc aaaaaggcct tttgttcatg gttatgcccg gttggtacgc
    11041 tttctgaatt aatcggcgct ctcggtaaca aactgtttgg tcggcaatgt gtccttcccc
    11101 gctggctgga tattcctctg cgcggtgtaa agtatttgct gttgagtttt tttctctata
    11161 tcgcgttatt gatgcctgct caggcgattc actattttat gttgtcgccc tacagcgtgg
    11221 tgatggacgt taaaatgctc gatttctttc gtcatatggg gaccgcgaca ttaatcagcg
    11281 tgaccgtttt gctgattgcc agcctgttta ttcgccatgc ctggtgtcgt tatctttgcc
    11341 catatggcgc gctgatgggc atggtttcgc tattatcgcc gtttaagatt cgtcgcaatg
    11401 ccgaaagttg tatcgactgt ggcaaatgcg caaaaaattg cccatcacgg atcccggtcg
    11461 ataaattaat tcaggtacga acagtggaat gtaccggctg tatgacttgc gtagagtcat
    11521 gtccggtagc ctcaacattg accttttcac tgcaaaaacc tgcggcaaat aaaaaagcct
    11581 ttgcgttgtc tggctggtta atgacgctac tgattctggg gattatgttt gcggtgattg
    11641 gttacgcaat gtatgcggga gtatggcaaa gcccggtacc ggaggaattg taccgacgct
    11701 taattccaca agcgccaatg attggtcact aaattagcaa caagcggaac atcacctcgc
    11761 acggggtgat gttcacaaaa tcacattatt ttgattttga catcataact ttcaagcccg
    11821 gtcatttttt ctcgtgcggt gaagcgaatt tcagagactt ctttccctgt attcttgttc
    11881 agctcggcga ttttttgcgc gataaagtcg gcgatttccg tttcggcttg tttttttaac
    11941 tcttcaatat tcactgttaa cctcttctgg tcttaaaaga cagcaacacg ccagtaaggt
    12001 gttggttccc ggtaccctgg tcaggtaaga aatgttataa gccatctccc cgaatgattc
    12061 aataatcaac aggttatcgc ttactataca tacagcgttg aaaaaaaata agacagatat
    12121 aaaaaaggct ccacgaggga gcctgatatt attttgtgaa atccagcggt gcatggtcag
    12181 tgcgtatttg tagagcaacg ttatcaaagg tgatcatcgt cgatttacaa aaaatacact
    12241 tagcgcccaa agggtttcgc tccgtcacat cgaatgccga tgtgcggtac tgtgaaccat
    12301 gacaacatgg acagcgaaaa tggagatgtt gagtaataac ggcaacctta gagcgtaact
    12361 acgttcgctg ccgcggggcc acgttgcccc tgctcaatag aaaattcaac tttctgattt
    12421 tcgttcagcg tgcggaattc attgctctgg atggcggtga aatggacgaa aacgtctttg
    12481 ctgccatcat caggagtgat aaagccaaaa cctttatctg cgttaaacca ttttactaaa
    12541 ccagtcattt tattagacat attttacttc cttcattttg ggcctttcgg caaacagggc
    12601 tttaatacag aaactactta gtgttcagga gagagactca aaagaagggg ataatgcctg
    12661 ataatgagaa ctgctttagt aaactacttt gtatgctgtc tgtctttcaa accgacgcag
    12721 ctattaacgc atgataattg agattaagca aattttattt tagccgtccg gcggtcttaa
    12781 atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt cagtaaggaa
    12841 tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa agcggcaaag
    12901 gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc gcattcactc
    12961 cccgctacgc agaagtgctt ataccaggat tacgcgtgga attttgccgc gtaaatggcc
    13021 tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct tgtttaaagc
    13081 tgactggttt tattggcata gttcaatagt caacaccatg tattataaca taagttactt
    13141 tttcaacagg aaattaatc
//
LOCUS       DAPGRN010000127        12541 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPGRN010000127 DAPGRN010000000
VERSION     DAPGRN010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.129"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..1380
                     /locus_tag="RHW33_003925"
     CDS             85..1380
                     /locus_tag="RHW33_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647381.1"
                     /translation="MYEAYNNGYPSPYGNVLHLKGATAAGEGELFIGWSGTSGAHAPV
                     HIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSGNAATATRLQTARTINGVSF
                     DGSKNIELTAENLNLEQTVELAAGSLQKNQNGADIPGKDTFTKNIGACRAFHSSISTG
                     AGNWTTAQLIEWLDSQGAFNHPYWMCKCSWSYGNNKIITDTGCGTIHLAGCVIEVMGN
                     KGAMTIRVTTPSTSTGGGTTNAQFTYINHGADYAPGWRRDYSTKNQQPAFALGQTGST
                     VGNDKAVGWNSISGVYNANIGGASTLILHFYMGAGSCPAVQFRVNYKNGGIYYRSARD
                     GYGFEADWSEFYTTTRKPSAGDVGAYTKAECNSRFITGIRLGTKSSVQTWNGPGWNDK
                     SGYVVTASINSNKDELIDTTQARPVQYCINGTWYNAGSI"
     gene            1383..1916
                     /locus_tag="RHW33_003926"
     CDS             1383..1916
                     /locus_tag="RHW33_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1647382.1"
                     /translation="MQHLKNIRSGNPKTVEQYELTRQFGVVWLYSEDGKNWYEEQKNF
                     QDDTIKIAYTSDGVIVAINKDVSTINPEGLSVVEVADITANRRADISGKWMFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTAWMLYAQKV
                     ESTDTSILPVTFPEQPE"
     gene            complement(1944..2471)
                     /locus_tag="RHW33_003927"
     CDS             complement(1944..2471)
                     /locus_tag="RHW33_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1647383.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKHFDVIWFFTEDGKNWYEEQKNF
                     QDDTIKLAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDGAVI
                     KRIYTAEELQQQVENQKARLLADAESVIQLLERAVRLNMATDEDHSRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(2476..4329)
                     /locus_tag="RHW33_003928"
     CDS             complement(2476..4329)
                     /locus_tag="RHW33_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647384.1"
                     /translation="MSTANTWSARQTFNGGITGALTGNADTATKLKTAININGVRFDG
                     SADITLTPKDLDVYSKSEIDNKKGMRKYTFSAPANAVSGKWYPIVFRRSGGSTDELAS
                     RVVITTYSSAGGYAMNNCEFNGFVMPGGWSDRGSYAAGFFSIYSTAERAIHSIISSVK
                     DDDLCSVFYVEARAFPIKIFAEEGLNVIVPTTDYTVGQATYKWGATDPVAESTNTQTI
                     LDFKNGRGYYCSHQFVSSLSGNAATATKLASSINIGGVSFDGSADIDLPGVNTKGNQD
                     TTGNAATATKLQTACTINGVSFDGSKDIELNPRSIGTINSTTMSFSGGAGWFKLATVT
                     MPQASSVVYINLIGGAGYNVGSPQQAGISELVLRAGNGNPKGITGALWRRTSVGFTNF
                     AWVNTSGDTYDIYVEIGNYATGVNIQWDYTKDATVQIHTSPTYTANKPTGLTDGTVYV
                     IYSSHIKPTAAEVGALSLSGGQLNGALGIGTSSALGGNSIVLGDNDTGFKQNGDGNLD
                     VYANNVHVMRFVSGSIQSNKTINITGRVNPSDYGNFDSRYVRDMRLGGASTYKPANNT
                     STWTHQAPSGCVYTGIIVQDTGSNSADNIGGVYYRPVQKQINGTWYNVAQV"
     gene            complement(4377..4937)
                     /locus_tag="RHW33_003929"
     CDS             complement(4377..4937)
                     /locus_tag="RHW33_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1647385.1"
                     /translation="MLIGYVRVSTNEQNTALQRNALESAGCELIFEDKASGKKAERPG
                     LKKVLRMLSRGDTLVVWKLDRLGRSMRHLVVLVEELRDRGINFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLDAARAEGRIGGRRPKYQEETWQQMRRLLEKGIP
                     RKQVAIIYDVAVSTLYKKFPASSFQS"
     gene            5065..5346
                     /locus_tag="RHW33_003930"
     CDS             5065..5346
                     /locus_tag="RHW33_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647386.1"
                     /translation="MFDLTIFFITILGGVHSFLNGVREKRYEASCRQLMAECIAAVLA
                     GFIGMYFAEYKGMDESLQNCVTIICSINNRLILEKLQRIIDSYLNRNAS"
     gene            5414..5743
                     /locus_tag="RHW33_003931"
     CDS             5414..5743
                     /locus_tag="RHW33_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HDV1647387.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            5740..6183
                     /locus_tag="RHW33_003932"
     CDS             5740..6183
                     /locus_tag="RHW33_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HDV1647388.1"
                     /translation="MIGWGVCVLALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            6170..6772
                     /locus_tag="RHW33_003933"
     CDS             6170..6772
                     /locus_tag="RHW33_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HDV1647389.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            6774..8693
                     /locus_tag="RHW33_003934"
     CDS             6774..8693
                     /locus_tag="RHW33_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647390.1"
                     /translation="MEQLNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGEVAQSGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLVAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRDTIDPAGMTGGHSRALTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALDLATSSPDEF
                     RVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            8690..9055
                     /locus_tag="RHW33_003935"
     CDS             8690..9055
                     /locus_tag="RHW33_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HDV1647391.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGGSGEEKQPSVHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            9068..12055
                     /locus_tag="RHW33_003936"
     CDS             9068..12055
                     /locus_tag="RHW33_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647392.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHINDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESVSGGSYNG
                     VAPYTSLLLHRASGTKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHTAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSQVNKHVGDIDGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRRMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            12045..12356
                     /locus_tag="RHW33_003937"
     CDS             12045..12356
                     /locus_tag="RHW33_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647393.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDDDDDDDDFGDHVDQIV
                     Y"
BASE COUNT         3417 a         2795 c         3133 g         3196 t
ORIGIN      
        1 ggtgacatta atatcaatac tctggtatcg cgcggtcgcg taacggcctt ggaggcgaat
       61 gcacagggaa catctgggat tcagctgtat gaggcataca acaatggcta cccttccccc
      121 tatggcaatg tgcttcacct taaaggtgcc accgctgctg gcgaaggtga gttattcatt
      181 ggctggagtg gcacgagcgg tgcccatgcg cccgtacata tccgttcgcg gcgggatact
      241 gattctgcca actggtctga atgggcgcag gtctatacgt caaaagattc aattcccggc
      301 gtcaatgcca aaggggatca ggatacctcc ggcaatgctg ctacggcgac cagattgcag
      361 acggcgcgca ctattaacgg tgtttcgttt gatggttcta aaaatattga gctaacggct
      421 gaaaatttaa atcttgagca aaccgtagaa ctagccgctg gatcattgca gaaaaaccag
      481 aatggcgcgg atattcctgg aaaagatacc ttcactaaaa atattggtgc atgtcgcgct
      541 tttcacagtt ctattagtac aggtgcaggg aactggacaa cggcacaatt gattgaatgg
      601 ctggattctc aaggggcatt caatcaccca tactggatgt gcaaatgttc atggtcgtac
      661 ggcaataata aaattataac cgatactggc tgtggaacta ttcatcttgc aggttgcgtt
      721 attgaggtta tgggtaataa aggtgccatg accatccgtg taacaacacc aagcacttcc
      781 accggaggtg gcaccactaa tgcgcaattt acttatataa accatggtgc agattacgcc
      841 ccaggctggc ggcgcgacta cagcacaaaa aaccagcaac ctgcatttgc tttagggcag
      901 acaggaagca cagttggaaa tgacaaagct gttggatgga actcgattag cggggtttat
      961 aacgctaata ttggtggagc atctacgttg attctccatt tttatatggg agctggtagt
     1021 tgtccggctg ttcagttccg cgtgaattat aagaacggcg gtatttatta tcgttctgcg
     1081 cgtgacggtt atggttttga agccgactgg tcggaatttt atactacaac acgtaaacca
     1141 tctgcgggag atgttggggc atataccaaa gcagaatgta attcacgatt tattacaggt
     1201 attcgattag gtacaaaatc gtctgtgcaa acatggaatg gtcctggatg gaacgataaa
     1261 tctggttatg ttgttactgc ctcaattaac tcaaataagg acgaacttat tgatacaact
     1321 caggcaaggc cggtgcagta ttgtatcaat ggtacatggt ataacgcggg aagtatttaa
     1381 ttatgcagca tttaaaaaac atcagatcag gtaatccaaa aactgttgaa caatatgaat
     1441 tgacgcgaca atttggtgtt gtctggttgt attctgaaga cggtaaaaac tggtatgagg
     1501 aacaaaagaa cttccaggat gacactataa aaattgctta cacatcagat ggagttattg
     1561 tggctataaa taaagacgta tctacaataa atccagaagg tttaagtgtg gttgaggttg
     1621 ctgacattac tgcaaatcgc cgtgctgaca tttctggtaa atggatgttc aaagatggcg
     1681 tagtgataaa gcgaacttat accgaggaag aacagaggca acaagctgaa aatgaaaagc
     1741 aaagcctatt gcaacttgtc agggataaaa cccaactgtg ggactcacag ctacggctgg
     1801 gcatcatttc cgatgagaat aaacagaaat taaccgcgtg gatgctatat gcgcagaaag
     1861 tagaatctac agatacctcc atcctgccag taacgtttcc cgaacaacca gaatgagaaa
     1921 aggcccgtta tcgggcctta tttttactca ggtttttgtg gccattctgg ctttgccgta
     1981 tccacgcggc ttacaagaac gctgtagcgt tcccatgctt ccagtcggct gtggtcctca
     2041 tccgttgcca tattcagcct tactgcacgc tctagtagtt gaatgactga ttcagcatca
     2101 gcaagaagtc tggctttctg attttctacc tgctgctgta attcctctgc cgtataaata
     2161 cgtttaataa ctgcgccatc cttaaacatc cagttaccag aaatgtcggc acgtcggtta
     2221 gcagtaatat ccggcacttc aacaacactt aatccatccg gtctgatagc tgttacatcc
     2281 ttttccacat agcggatgat gttatctttg tcatacgcta attttatcgt gtcatcctgg
     2341 aagttctttt gttcctcata ccagttttta ccgtcttctg taaaaaacca gatgacatca
     2401 aaatgtttcg tcagctggta ttgctcaact gtttttggat tacccgctgt tatattgatc
     2461 aaatgctgca taaatttaaa cctgtgccac gttataccat gttccattaa tctgtttctg
     2521 aaccggcctg taatatacac ctccaatatt atcggcagaa ttcgagccgg tatcctggac
     2581 tataatgcca gtataaacgc agcctgatgg tgcctgatgc gtccatgtcg aggtgttatt
     2641 tgctggttta tatgtagatg ctccaccaag tcgcatgtct cttacataac gagaatcaaa
     2701 gttaccgtaa tccgaggggt taacacgccc tgtaatattt atggttttat tactttgaat
     2761 gctgccggag acaaagcgca taacatgaac gttattagca taaacgtcca gattaccgtc
     2821 gccattttgt ttaaagcccg tgtcattatc acccaaaaca atcgagttac cgccaagagc
     2881 actggatgtt ccgataccca atgcaccatt caattgaccg ccagataatg acaatgcccc
     2941 aacctcagca gcagtcggtt taatgtgcga actgtaaatt acatatacag ttccatctgt
     3001 caggcctgtt ggtttattcg ctgtataagt tggtgatgta tgaatttgta cagttgcatc
     3061 ctttgtataa tcccactgga tattaacacc tgtggcgtaa ttacctattt ctacataaat
     3121 gtcataggta tcaccggatg tattcaccca tgcaaaatta gtaaatccaa ccgaggtccg
     3181 tcgccataat gcaccagtaa taccttttgg atttccattt cctgcacgca gaaccagctc
     3241 agagatgcct gcttgctgcg gggagccaac gttataccct gcgccaccaa tcaggtttat
     3301 gtaaaccacg gaactggctt gtggcattgt tacagttgcc agcttgaacc atccagcacc
     3361 accactaaaa gacattgttg tggagttgat cgtgcctata gaccgtgggt taagttcaat
     3421 atctttagaa ccatcaaacg agacgccgtt gatagtacat gctgtctgca acttggtcgc
     3481 tgtcgctgca ttaccggtag tgtcttgatt accttttgta ttcacaccag gcaaatctat
     3541 atcggctgaa ccatcaaagg atacaccacc tatgtttatt gaacttgcta atttcgttgc
     3601 tgttgctgca tttcctgaaa ggcttgaaac aaactgatgt gagcagtaat aaccacgtcc
     3661 atttttaaaa tccagaatgg tttgagtatt tgtactttct gctactggat ctgtggcccc
     3721 ccacttatag gtggcttgac caacggtata gtcagtagtt ggaacaatta cgttcaagcc
     3781 ctcctcagca aatatcttga taggaaatgc tctggcttca acgtaaaata cactacacaa
     3841 atcgtcatct ttcacacttg aaataatcga gtgtatagct cgctcagcag tagaatatat
     3901 tgagaaaaaa ccagccgcat atgaaccgcg atcagaccaa ccgccaggca taacaaaccc
     3961 attaaattca caattattca tggcgtatcc gccagctgag gaataagtag ttatcacaac
     4021 tcgtgaggct aattcatcgg tactgcctcc agatcggcga aagactatag gataccactt
     4081 accgcttact gcatttgcag gcgctgaaaa agtgtatttt cgcatccctt ttttattgtc
     4141 tatttcgctt ttgctgtaaa cgtccagatc tttaggagtc aacgtaatgt cagccgaacc
     4201 atcgaacctg acgccattaa tgtttatggc tgtttttaat ttcgtcgcgg tgtcggcgtt
     4261 ccctgtcagc gccccggtga tcccgccgtt gaaagtctgg cgcgcactcc atgtgttagc
     4321 cgtgctcaac agggggatct tttcaccgct ggtaccgagt tctcttaaac caaggtttag
     4381 gattgaaatg atgacgccgg aaacttctta taaagagtgg aaacagccac atcatagatg
     4441 attgcaacct gcttacgggg gatgcccttc tccagcaatc gccgcatttg ctgccatgtt
     4501 tcttcctggt atttaggccg acgcccacct attcgacctt ctgcgcgagc tgcatcaagt
     4561 ccagctcgtg tacgttcaac aataagttca cgttccattt ctgccagcgc ccccattacg
     4621 tgaaagaaaa agcgccccat tggtgtactg gtgtcgatgg agtcagtgag actccggaag
     4681 ttaatgcctc tgtcacgcag ctcttccacc agcacaacta agtgacgcat gctgcgccca
     4741 agacggtcta acttccatac gaccagggta tcacctctgg aaagcatacg gagtaccttt
     4801 tttaacccag ggcgctcagc ctttttgccg ctcgccttgt cctcaaaaat tagctcacat
     4861 cctgcgcttt caagagcgtt tcgttgtaaa gcagtgtttt gttcatttgt tgatacgcgt
     4921 acatagccaa ttagcatatt ttctgctcac catagttatt catagcaagc tgcggatttt
     4981 aattaacaga aaccagtgtg tgtggaaatc acaaagtgca taccgtgccc caatgatgtt
     5041 ttattgaaaa gcggggttac tttaatgttt gatttaacca tattttttat aaccatcctt
     5101 ggcggggtgc attcgtttct gaatggggtt cgtgaaaaac gttacgaagc gtcatgcagg
     5161 caattgatgg ccgagtgtat tgctgccgta cttgcaggct ttattggcat gtatttcgcg
     5221 gaatataagg gcatggatga aagtcttcag aattgcgtga ctattatttg cagcatcaat
     5281 aacaggctca ttcttgaaaa gttacaaagg attatcgatt cgtacctcaa tagaaatgcc
     5341 tcttaagcaa caaatgaccg gttgagaagt tactttgcat accattacct cctgacaacg
     5401 taggagggaa cttgtgcttg acacacagga attagctcca gttgctattg cgctcctgct
     5461 ttcagtaatt ggtgggatag gcacgttcct gatggatgtc cgagacggtc gccagtctgg
     5521 caatttgttg ggattggtta cggagatctt tgttgcagtg acagctggcg cggtggcgta
     5581 cctattgggg caacacgagg gctgggagtt atcaattacg tacttaatgg taacgatagc
     5641 cagcaataac ggtcatgagg tgatttcagg gatgaaacga gtgaatatcg atagcattct
     5701 gaatgttctt acaagtttgg tgaaaaaggg aggcgggaaa tgattggctg gggtgtatgc
     5761 gttcttgcgt tagccttagc cgatcgctat ttgctaaaac gcaaggacat cacgcattta
     5821 gaacttggtg atgtggaaat taaaccgggt ttcatccggg tgccgttcaa gtaccggtct
     5881 aaattcccgt ttttgcgcgg cgcaacggtc agatattgga tccgcgatgt tcagaagccg
     5941 acgacagtga ttgaaggtga acaacgttgt ctgacgtcgg ctgaacaggg cgaaaacagt
     6001 gaatggttgt acatacccac tgaatatatg ggtaaaggag agcgactgtg gcatttcaac
     6061 gtcatggtta cgcatggcga ctcgttcatt aacccgttgt atcggatttt ccctgttact
     6121 cagcaaatcc gcagaagtta cgtaataaat ctcgcacagg atgtgtcaga tgacgaaaaa
     6181 taagtatgca acggtcgatt ttgaccaggt taatgaaaag gggctgaaat cccttatcgc
     6241 ggcgatcaat aaaaccggtg ttacggtaat tgaggttgac tccagcaacc gcgcaacaac
     6301 gaaagatggc gttaaagtta aaaccgcaaa gctggttctt aacgacggac aaattcttgc
     6361 catacaggta aacgatactg gcgatatatc gtctgtgaag ctgaatggaa aagctattcc
     6421 taacgctcag tcgccggata tcaagacgct tggtaccgtc atgggacagg cggcccgcaa
     6481 aaactccgca aaattccaga aatcactgat cgccaaagcg aagcgtgttg ccaatccggt
     6541 agacaagaaa ccggcagtta aatccaactt tcagcgcctg caagaagcaa aacagcggaa
     6601 tgctcaggtg gttgccgctt ataagtccgc gcagaattcg gtgtctttca accaacagca
     6661 gatcactgat ttgcgggcga agctggataa ggagacgggc cgactcaata acgaaaaggc
     6721 acggaatggc gaactcaaac gtcgtcttaa gcaactgaaa gcaggaaatt aacatggaac
     6781 agttaaatat caataaaggg gtgacgatca agcctgggct tgacgtgctt cccccgccag
     6841 tgactgatga tgaatatcgc gcattaatgg ccggtgagga ccgctatctg atgacggaat
     6901 ccaacaccct ggaggaaatc gaggctacgt tcttctatga cacgccgatc cactggtgtg
     6961 ctacggattt actggaggcg attagttcta ctcgtttgca gttacaccgg accatgcagg
     7021 catttgtccg ggcattgaac cagaagctga atggtaccgg aatctctgcg gggagtgata
     7081 aaacggggga ggtggcccag agcggtgcgc gcgcgatcgg cggtgctgaa attggccggg
     7141 cacgtaacgt taacgggctg ccggtcctgc cagccattat tccgctcagt gatggtcaga
     7201 ctatcagcat tctgtttcat agcccgacag cggaaaaccg gatcaccaat agcgatacgc
     7261 tggttgcttt ccagttctta ctgaataaaa aagacgttac tcacaccgtt gctccgatga
     7321 gtggacgtga tatgacgctg gcgcaggtca ccatgaaact tgccaacctt gcagagaaaa
     7381 actcggcaaa attccagcgt gcgcagaaga agaaaaaagc cctggttgat gaaataaccc
     7441 aactacaggc tgacagtgac cagaaagagg atgccatgag cgacctcgcg gatcaggtgg
     7501 cagcggtaga agggcagaag gtagatctgg agcagaaaat taacgctgtt gcatcggaag
     7561 cggattctct ttatgaagag aatgagcgtt tgcagacgga gattgatcag ctcaatcgca
     7621 caggtgggcg cgataccatt gatccagcgg ggatgactgg tgggcactcg cgcgcgctga
     7681 cggatcgcct tgccagtatc aaaaatcgta tgcatatgaa cggggaagtg acgctcagta
     7741 atggagcatc aatgaagcaa ttcattgagg acggcgaagg gtatatccag ttaaccgatt
     7801 cggatggcag cgtgtacatg atcaaggcta aatccataca gggtgtggac atggcagatg
     7861 cgatcggcaa gctgtttaaa gcctataaag cgggtaatgt atcggaatat ctggtccaac
     7921 cagaagaaca taaaccggaa aacgtcgaac ctgaaccagc ggaggatacc ggtagctctt
     7981 cgcctgaacc agaagtctct gtaggtgcat atcgatatgc cttgcaaatg cgtccggcgg
     8041 cccctggcgc aatacctgaa ggcaacaaag caattctgcc gcgccctgat gaaggtgacc
     8101 cgtattatga atatgcacgc tacggcattg ctacttacga taccccgctt tctgatcagc
     8161 aaatgagtga gtacgacctg aagttattgc ctcgcgagga ttctttcgac ttcctggcga
     8221 agacacttac taatggtccg tttggcaaat atgcacaaaa agctctggat ctggccacca
     8281 gctcaccaga cgagttccgc gtaatgctga aaactcagtt tcaaaaaact ttccccaata
     8341 ttgcgtttcc gggtggcgct ggcaccgaga aaatggtgca gagcatgatc aatgcattgc
     8401 aggccgaagt cggtgagatt actcagccag aaccggcccc ggcacagcct gatgaaacgg
     8461 ttagcgaagc agatgcagag gctaataaag ccattgaata tctcaataac gtgatggata
     8521 tgcaaagcac tgacatggcg gagatccgta acgcccgggg caatgtccgg gaagcgattg
     8581 cagcccttca ggctgccggg cgttttgagg aaaacgaaga gctggttaat ggcgctgctc
     8641 gccacctggc tgatctgctg gtagcaatcc agaaagcggg ggtagcggca tgacactatc
     8701 agctattgag ttaatggatc tcagcgataa gttggatgct ctgatgtcca aagcggctac
     8761 cgcgagtggc atggagttgc tggatatcag cgatgaaatt gaccagatca tgcaacagat
     8821 ggggtacggc gcgtccggcg gcggtagtgg cgaggaaaaa caaccttcgg tacatgatgg
     8881 tgtgccaaaa ctggttgctg atttcctggc tgataaattc gtcgatcaga gcaccgatgc
     8941 atttatcggt acgttgcagg acttgagtca atatgttggc acatacatcg atctggacca
     9001 ggttaaacaa cacacggcgg catggatagc cgctaacatt aaagaggcag cataaggcgt
     9061 aacagggatg agcttaagcg atcaggtggt gatggccacc agcatagaaa cgctgatcga
     9121 gctgctaaaa aacctgcccg attacgggcg ggtttcgtat gtggtgacag cgaagggaga
     9181 cgaggtaaaa acagcgtttg atatcgtcga tgcctcagct cttttggtat ccaatactct
     9241 ggacgggaaa attaatccgg actatcccca ggaacttcag ccgcgcgacc ggacccgcgc
     9301 atccagcctt cttcaggtca accagatatc caaggatttg cggcctgctc agcttaccga
     9361 ttccggttta tccagccatg gcgcgccgat aattggtgag gacaatgccg ttgagtcagg
     9421 taatggacgg accatgggga tcatcaaagc ctatcaggac ggcaatgcgg atcggtatcg
     9481 tgagtacctg attgaacatg cgaccgaatt cggcatacga cctgaaaagg ttgaatcaat
     9541 gacggctccg gtactggtgc gccgccggtt aacgaaggtt gaccgtgttc agtttgccaa
     9601 ggactcaaat atttctgatc tccaggaaat ggcagccagt gaaaaggctt ttgttgatgc
     9661 cgacagcata actccggcga tgatggcgct gtttaaccca tcagaaagcg gagatctgct
     9721 tagccgcagt aatgacgcgt ttattcgcgg attcatgacg caagttggtg ccacacaggc
     9781 tgctggcctt gtaacggaag atgggcgacc aacacgacaa cttgttgacc gtatacaaaa
     9841 cgcgatcttt gccaaggcat ataaggatgc gcgcctggta agaatggttg cagaagaacc
     9901 tgatccggat atgcgtaatg ttctgacggc gcttaatgcg gcagccaatg attttgtcca
     9961 gatgcaggct ttatcaggag aagcgcacaa gcaggctgtg acaactatcg ttgatggtat
    10021 tgaaacagcg gatagcctcg ataaaaaggc gctggcggca ttgaaagatg cggtagacct
    10081 ggtaaggcaa tcgaaggagt caggccaaca tattaacgat gttattgctc agggggatat
    10141 gttcagcgaa acagccccgg aagtgaaagc tctcgcgttg ttcatcgtcg cgaataaccg
    10201 tagcgcgaag cgtatggcca ccgcctttaa attgatggct caacgtatca atgatgagtt
    10261 acagcaccag ggccaggcgc tgggggatat gtttggcggt ggtgatgtgt cgttacagga
    10321 tatccttcgc caggtgtctc aggaactgga aaacgaaggc atgcaaggga tatccggcgg
    10381 tcttttcgag tccgtttccg gcggtagtta caacggcgtt gctccatata ccagtttgct
    10441 attgcatcgg gcatccggca ccaaagacat tattcatctg atcaggctgc tttcccgtac
    10501 agatccccag gatgaacagc ttgttcaagt gcttgcgcat tttgttcgaa tgcctgttgc
    10561 cgacgtgaaa aaatggtgcc gattattcgg tatcagcaat tcgttacttc gcggcttgtt
    10621 aaatcacgca tcctcccttg ggcgcgatgg ctttgacgag atagcgcagg cgataaaaaa
    10681 cggagatatg ccaccagcta ttgactggtt ttccattcgc ccaaccaggg tgaaagcatt
    10741 ccttagcgcg gcgcatacgg catcaccatt ggcagaaatg gttcagaggt tgtcgctcat
    10801 attcacagac cataccgcgt tgggtgatct gactctggac gagatgaaag aagcctccat
    10861 tcagtgggcc gatcaacaaa atgaggttaa ctctgacttc ttgccagcat tcaggaaggc
    10921 cgttagtaaa gctgatgatg cccgtggaat tctgaaggca tttaaggcat tgcaaagtca
    10981 ggttaataaa catgtcggtg atatcgatgg ggtaacggcg gaaggcaggg atatccttaa
    11041 agagcacggc ataacgccag agtttattga tgagatcagg actgatatgc agcgtgaggt
    11101 cgtatcgtca ctgcaaatcg tagccagagc gttggcggat gctaatccga agagtgcggc
    11161 cattgttaac cgtgttattg gtgatattga agcatcggag ggcatggggg cgctgaaact
    11221 cttcctttcg cgagcgttta atcctaacgg caatattctc cccggcatta ttggtgaggc
    11281 taaaaagtat gtcagcgaag aagaacttga gcagcttgac caactactta agcgattctc
    11341 atataacccg cagacacgct ggcaaatgaa tcagcgaaga atgggttcgg tccacgagaa
    11401 agtgttatct gccatgaaca gtgcgatcgc aaactcatcc gtatctgaag aaaaagctct
    11461 tgagtgggcc gactctttta tcacggaaga agtggaagaa gcccgcgctg gacagaatgg
    11521 tgggatagac ctgcgcaagg aacttgctga tatttatcgc ctgaccggcg gtaaaatttc
    11581 gaccttatca aaggtggttc accaccaggg aagggcatat gcaaatctta atggtgttgt
    11641 tgctgtcaat ttgaacgatg aaaatgcaag tgcactgtgg cacgagctgg gtcatcatct
    11701 tgagtacagt aaccctggtt tgttagagaa agcccggtca ttcctgaagg ccaatgttga
    11761 aggggataag ccatctttcg ttaatatcgg tgggcgtggc aagcctgaat ggtgcttcag
    11821 atctcgattg agtaatattt atatggcgaa ggtatacccg ccagcctcag taagtaacac
    11881 cgggaaaatt cggcagaaat caccgactat ttccaaaacg tcagcaacgg aagtattctc
    11941 tatggctctt cagttgtatc atgacaaaga ggccgctgcc gcatcactga tgaatggtga
    12001 cggattgctg gaactgttat taggtgtggc aaaggagcta aataatgcag attaaaatcg
    12061 cagcgccatt aggcggagat gccattatcg aatttgatga taatgaagaa gtttccgggc
    12121 gtttaagcat tatctccggt gacattaccg aggacatgat cgctgaagcc atagctgggg
    12181 caaatcccaa tagctatatg ggattcgtta acacccttga tgctcccgca agtgatgttc
    12241 tccgaacgct gcatctttac gctggctggt ttgttgattg gccagcagta gatggtggcg
    12301 atgatgacga cgacgatgat gattttggtg atcatgtaga ccagatcgta tattaactcc
    12361 ctgattgtgc aaaaataatc tgttctgata tgttaattgt gtacttaaag taaacgcgta
    12421 gtggcttgct ttaggttatg gaagcaagcc gttacctaat atgttagatc aaaaaaaatt
    12481 aatttttgcg tttcgctcac cacatattga acactttagt cgatctttta attcttccag
    12541 a
//
LOCUS       DAPGRN010000128        12428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPGRN010000128 DAPGRN010000000
VERSION     DAPGRN010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.130"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            250..1638
                     /gene="narU"
                     /locus_tag="RHW33_003938"
     CDS             250..1638
                     /gene="narU"
                     /locus_tag="RHW33_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV1647394.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAR
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1720..5460
                     /gene="narZ"
                     /locus_tag="RHW33_003939"
     CDS             1720..5460
                     /gene="narZ"
                     /locus_tag="RHW33_003939"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV1647395.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASGGIKPEDVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5457..7001
                     /gene="narH"
                     /locus_tag="RHW33_003940"
     CDS             5457..7001
                     /gene="narH"
                     /locus_tag="RHW33_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV1647396.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDHNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7001..7696
                     /gene="narW"
                     /locus_tag="RHW33_003941"
     CDS             7001..7696
                     /gene="narW"
                     /locus_tag="RHW33_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV1647397.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQRKAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            7693..8373
                     /gene="narI"
                     /locus_tag="RHW33_003942"
     CDS             7693..8373
                     /gene="narI"
                     /locus_tag="RHW33_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV1647398.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8452..9345
                     /locus_tag="RHW33_003943"
     CDS             8452..9345
                     /locus_tag="RHW33_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV1647399.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKALGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGKTRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9441..10286)
                     /gene="nhoA"
                     /locus_tag="RHW33_003944"
     CDS             complement(9441..10286)
                     /gene="nhoA"
                     /locus_tag="RHW33_003944"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV1647400.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREMQLDDQSLEEKLVIARRGGYCFEQNGVFERVLCELGFHVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10459..11028
                     /locus_tag="RHW33_003945"
     CDS             10459..11028
                     /locus_tag="RHW33_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV1647401.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGRDEDKFNCY
                     GIPVVRGPVFGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11032..11259)
                     /gene="pptA"
                     /locus_tag="RHW33_003946"
     CDS             complement(11032..11259)
                     /gene="pptA"
                     /locus_tag="RHW33_003946"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV1647402.1"
                     /translation="MPHIDIKCFPRELDEQQKAALATDITDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11570..>12428)
                     /locus_tag="RHW33_003947"
     CDS             complement(11570..>12428)
                     /locus_tag="RHW33_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV1647403.1"
                     /translation="MRDCHSSNDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHS
                     LVIGQIRTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAV
                     KGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREETRLYIVCEVPDELIDFTFE
                     WKGLKKLCVAVTFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHW
                     RLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLA
                     SVLAGSGLS"
BASE COUNT         2950 a         3220 c         3179 g         3079 t
ORIGIN      
        1 atcatctgaa gggttgtatc atgacatgcg ttcaaagatt accccccgtt taatggttag
       61 cttgtcagtt acatttagta acacatatct ggctacctaa tccccattct gcccccgctg
      121 ttacgctttc ttatctattc tttcaatcag cacttcaccc aattcatcct taattctaat
      181 aactggctat tgcgccttac ctcctgtttt gtcttttttc attaccaatg tgtgcatgtg
      241 aggaacaata tggcactgca aaatgagaaa aatagtcgtt atcttttacg cgactggaaa
      301 ccagaaaatc cggccttctg ggaaaataaa gggaaacata ttgctcgaag aaacctctgg
      361 atatcagtca gttgtctact tcttgccttc tgtgtctgga tgctatttag cgcagttacc
      421 gttaatctca ataaaatcgg ttttaatttt actaccgatc aactcttttt attaaccgca
      481 ttaccctccg tttctggcgc attattgcgt gttccctact cctttatggt acctatattc
      541 ggtggacgcc gatggacggt ttttagtact gcaatcctga ttattccttg cgtctggctt
      601 ggaattgccg tgcagaatcc gaatactcct tttgggatct ttatcgttat cgctttgcta
      661 tgcggttttg caggtgcgaa ctttgcttcg agcatgggca atatcagttt cttctttcca
      721 aaagccaggc aagggagcgc tcttgggatt aatggcggat taggaaactt aggtgtaagt
      781 gtgatgcagc tggttgcacc gctggtcatt tttgtacccg tatttgcctt tctcggcgtc
      841 aatggcgtcc cgcaggccga cggttcggtg atgtcgctgg cgaatgccgc atggatttgg
      901 gtaccgctac tggcaattgc cacgatcgcc gcctggtcag ggatgaatga tatcgccagt
      961 tcacgcgcgt caattgccga ccagctccct gtcttacaac gcctgcatct ctggctgttg
     1021 agcctgcttt accttgccac cttcggttcg tttatcggtt tttctgcggg tttcgccatg
     1081 ctggcaaaaa cccagttccc ggatgtgaat attctgcgcc tggcgttctt tggcccattt
     1141 atcggtgcca tcgcgcggtc ggtgggtggt gctatttccg ataaattcgg cggcgtgcga
     1201 gtgacgttga tcaactttat ttttatggcg attttcagcg ccctgctgtt ccttacctta
     1261 ccgggcacag gctccggtaa tttcatcgca ttttacgccg tatttatggg gctgtttctg
     1321 accgcgggtc tgggaagtgg ttctactttc cagatgatcg ccgtcatctt tcgccagata
     1381 accatttatc gggtgaagat gaaaggcggt agtgatgagc aagctcagag agaagctatc
     1441 accgaaacgg cggcggcttt gggctttatc tcagccattg gcgcagtggg cggctttttt
     1501 attccgcagg cgtttggcat gtcgctcaat atgaccggct ctccagtggg cgcgatgaaa
     1561 gtgtttttaa tcttctacat cgtttgtgtg ctactgacct ggctggttta tggtcggcgg
     1621 aagttcagcc aaaaataaga gatcgatgcc tgatgtacaa ccgtgtcgca tccgacaagg
     1681 catcagatcc caattcattc ctggagcagg agtcatgtca tgagtaaact tttggatcgc
     1741 tttcgctact tcaaacaaaa gggcgaaacc tttgccgatg gtcacggaca ggtgatgcat
     1801 agcaaccgcg actgggagga cagctatcgc cagcgttggc agttcgacaa aatcgtgcgt
     1861 tccacccacg gtgttaactg tacaggctcc tgtagctgga aaatctacgt taaaaatggt
     1921 ctggtgacct gggaaatcca acagaccgac tacccgcgca ctcgccctga cctgcccaat
     1981 catgaacctc gcggctgccc gcgtggcgca agttactcct ggtatcttta cagcgctaac
     2041 cgcctgaaat acccgctcat tcgtaaacga ctgattgaac tgtggcgcga agccctcaaa
     2101 caacacagcg atccggtact ggcgtgggca tcgattatga acgatccgca aaagtgcctg
     2161 agctacaaac aagtgcgtgg gcgcggcggg tttatccgct ccaactggca ggaactaaac
     2221 cagctgattg ccgccgctaa cgtctggacc atcaaaacct acggcccgga tcgcgttgcc
     2281 ggtttctcgc cgatcccggc gatgtcgatg gtttcttacg ccgccggaac acgttatctg
     2341 tcactattag gtggcacctg tttaagcttc tacgactggt attgcgacct gccgcccgcc
     2401 tcgccgatga cctggggcga gcaaaccgac gtaccggaat ctgccgactg gtataactcc
     2461 agttacatca tcgcctgggg gtctaacgta ccgcagacac gtacgccgga cgcccatttc
     2521 tttaccgaag tacgctacaa aggcactaaa accatcgcca ttacccctga ctactctgaa
     2581 gtggccaaat tgtgcgacca gtggctggca ccaaaacaag gcactgatag cgccctggcg
     2641 atggcaatgg gccatgtgat tttaaaagag tttcatctcg ataatcccag cgactacttt
     2701 atcaactact gccgccgcta tagcgacatg ccgatgctgg taatgctgga acctcgcgac
     2761 gacggtagct acgtacccgg gcgcatgatc cgcgcgtctg acctggtaga tggactaggc
     2821 gaaagcaata atccgcagtg gaaaaccgtg gcggttaata ccgcaggtga attggtagtg
     2881 ccgaatggtt cgattggttt ccgctgggga gaaaaaggca aatggaatct ggaatccatt
     2941 gccgccggta cggaaaccga attgtcgtta accctgctcg gtcaacatga cgctgttgca
     3001 ggcgtggcct tcccctactt tggcggcatt gaaaatccgc attttcgcag cgtaaaacac
     3061 aatccggtgc tggtgcgcca attgcccgtt aaaaacctga cgttagtcga tggcaacacc
     3121 tgtccggtgg tcagcgttta tgatttggta ctggcgaatt acggcctcga tcgcgggctg
     3181 gaagatgaaa acagcgcgaa agattacgct gaaatcaaac cgtacacccc agcctggggt
     3241 gagcaaatta ccggcgtacc gcgccagtat attgaaacca tcgcccgcga atttgccgat
     3301 accgcccata aaacgcatgg gcgctcaatg attatcctcg gtgctggcgt caaccactgg
     3361 tatcacatgg atatgaacta ccgtgggatg atcaatatgc tgatcttctg cggttgtgtc
     3421 gggcaaagcg gcggcggctg ggcgcactat gtcggtcagg aaaaactgcg cccacaaacc
     3481 ggctggttgc cgctggcctt tgcgctcgac tggaaccgcc caccgcgcca gatgaacagt
     3541 acctcgtttt tctacaatca ttccagccaa tggcgctatg aaaaagtctc tgcgcaggag
     3601 ttactttcac cgctggccga tgccagtaag tacagcggtc atctgattga tttcaacgtt
     3661 cgcgccgaac gtatgggctg gctaccttct gcgccgcagc tggggcgtaa cccgctcggg
     3721 attaaagctg aagccgacaa ggccggatta tcccccacag aatttaccgc ccaggcgctg
     3781 aaatcgggcg atttacgtat ggcctgcgaa caaccagata gcggcagcaa tcatccgcgt
     3841 aatttgtttg tctggcgttc taacctgctt ggctcctccg gcaaaggcca cgagtatatg
     3901 cagaagtatc tgctggggac cgaaagcggg attcaggggg aagaactcgg cgccagtggc
     3961 gggatcaaac cggaagatgt cgagtggcaa actgcggcga ttgaaggcaa gctcgacctg
     4021 ctggtgacgc tcgacttccg catgtccagt acctgcctgt tctccgatat cgttctgccc
     4081 accgccacct ggtatgaaaa agacgatatg aacacctcgg atatgcatcc gtttattcat
     4141 ccgctatctg cggcggtcga tccggcctgg gagtcacgca gcgactggga aatctacaaa
     4201 ggtattgcca aagctttttc gcaagtgtgc gtgggtcatc ttggcaaaga aaccgatgtg
     4261 gtattacaac cactgctgca cgactctccg gcagagctct cacagccgtg tgaagtcctc
     4321 gactggcgca aaggcgaatg cgatctgatt ccaggtaaaa ccgcacccaa tattgtggcg
     4381 gtggagcgcg actaccctgc tacctatgaa cgctttacct cgctcgggcc attgatggac
     4441 aaacttggca acggcggtaa agggatttcg tggaataccc aggatgaaat cgatttcctc
     4501 ggtaaactca attacaccaa acgtgatggc ccggcacagg ggcgtccact gattgacacc
     4561 gctattgatg catcagaggt cattctggca ctggctccag aaaccaacgg tcatgttgca
     4621 gttaaagcgt ggcaggcgct gggcgagatc accggacgcg aacataccca tctggcgctg
     4681 cacaaagagg acgagaagat tcgctttcgc gatattcagg cgcagccgcg taaaattatc
     4741 tccagcccca catggtctgg tctggaaagc gatcacgtct cctataatgc gggatacacc
     4801 aacgttcatg agttaattcc gtggcgcacg ctgtcgggac gccagcagct ctatcaggat
     4861 catccgtgga tgcgtgcttt tggtgaaagc ctggtggctt atcgcccgcc tatcgacacc
     4921 cgtagcgtca gtgagatgcg ccagatcccg ccaaacggct tcccggaaaa agcacttaac
     4981 ttcctgacgc cgcaccagaa atggggcatt cactcaacct acagtgaaaa cctgctaatg
     5041 ctgacgctct ctcgcggtgg accgattgtc tggatcagcg aaaccgatgc ccgtgaacta
     5101 accattgtcg ataacgactg ggtggaagtg tttaacgcca atggcgcgct gacggctcgt
     5161 gcggttgtta gccagcgtgt accgccgggc atgaccatga tgtatcacgc ccaggaacgc
     5221 attatgaata tccccggctc ggaagtaact ggcatgcgcg gcggcattca taactcggtc
     5281 acccgtgttt gcccaaaacc gacacatatg attggcggtt acgcacagct ggcctggggc
     5341 tttaactact acggcaccgt cggttcgaac cgcgacgaat tcatcatgat ccgcaagatg
     5401 aagaacgtta actggctgga tgatgaaggt cgcgatcagg tacaggaggc gaaaaaatga
     5461 aaatccgttc gcaagtcggc atggtgctta atctcgataa atgtatcggc tgccatacct
     5521 gttcggtgac ctgtaaaaac gtctggaccg gacgcgaagg catggagtac gcatggttta
     5581 acaacgtcga aaccaaaccg ggcattggtt atccgaaaaa ctgggaagat caggaagagt
     5641 ggcaaggcgg ctgggtccgc gatgtgaatg gcaagatacg cccgcgtctg ggcagcaaga
     5701 tgggtgtaat aaccaaaatc ttcgccaacc cggtggtgcc gcagattgat gattactacg
     5761 aacctttcac cttcgactac gaacatttgc atagcgcacc ggaaggcaaa catatcccta
     5821 ctgctcgccc gcgttcgctg attgacggta agcggatgga caaagtgatc tgggggccaa
     5881 actgggaaga actgctgggc ggcgagtttg aaaaacgtgc ccgcgaccac aacttcgagg
     5941 ccatgcaaaa ggagatgtac gggcagtttg aaaacacctt catgatgtac ctgccgcgcc
     6001 tgtgcgaaca ctgcctcaat cccagttgcg tggcgacctg cccaagcggc gctatctaca
     6061 aacgcgaaga agacggcatt gtgctgattg atcaggataa atgccgtggc tggcgtttgt
     6121 gcattagcgg ttgtccgtac aaaaaaatct acttcaactg gaaaagcggc aagtcagaaa
     6181 aatgtatctt ctgttaccca cgaattgagt ccggtcaacc gaccgtatgc tcagaaacct
     6241 gcgtgggtcg catccgatat ctgggcgtgc tgctttacga cgccgaccgc attgaggaag
     6301 cggcaagcac cgagcgcgaa gttgacctct atgagcgcca gtgcgaagtg ttcctcgatc
     6361 cacacgatcc ctcagtgatc gaggaagccc tgaaacaagg tattccacaa aatgtgattg
     6421 acgctgccca gcgttcgcca gtctacaaaa tggcgatgga ctggaaactg gcactaccgt
     6481 tgcaccctga ataccgcacc ctgccgatgg tctggtacgt tcctccgctg tcaccgattc
     6541 agtcctacgc agatgcgggc ggtttgccga aaagcgaagg cgtgctgccc gctatcgaaa
     6601 gcctgcgtat tccggtgcaa tatctcgcca atatgttgag tgctggcgat accggtccgg
     6661 tactgcgggc gctgaaacgg atgatggcga tgcgccacta tatgcgttca caaaccgtgg
     6721 aaggcgttac tgatactcgt gccatcgacg aagtaggcct gagcgtcgcc caggtcgaag
     6781 aaatgtatcg ctatctcgcc attgccaact atgaagatcg ttttgttatc ccgaccagcc
     6841 atcgggaaat ggcgggcgat gccttcgcag aacgcaacgg ctgcggtttt acctttggcg
     6901 acggttgcca cggttcggac agtaaattca acctgttcaa cagtagccgt atcgatgcca
     6961 tcaacatcac cgaagtgcgc gacaaagcgg agggcgaata atgcagatcc tcaaagtgat
     7021 cggcctgttg atggagtatc cggacgagct gttgtgggaa tgcaaggagg acgcgctggc
     7081 gttgatccgc cgcgacgcgc cgatgcttac ggatttcacc cacaacctgc ttaacgcgcc
     7141 gctgctggat aaacaggccg aatggtgcga agtgtttgac cgcgggcgca ccacgtcgct
     7201 gctgctgttc gaacatgttc atgccgagtc ccgcgatcgc ggccaggcaa tggtggacct
     7261 gctggcggag tatgaaaagg tcggcctgca gctggattgt cgggaactgc ccgattatct
     7321 accgctgtat ctggagtatt taagcatact gcctgacgat caggcgaaag aaggattgct
     7381 caatgttgcg ccgatcctcg ccctgcttgg cggtcgctta aaacaacgca aggcaccgtg
     7441 gtacgcgttg tttgatgctc tgctgcaact ggcaggaagt acactttcaa gtgacagtgt
     7501 cacaaaacaa gtaaacagcg aagagcgcga tgacacccgc caggcgcttg atgcagtgtg
     7561 ggaagaggaa caggtgaagt ttattgaaga taacgccacg gcgtgtgaca gctcgccgct
     7621 taatcaatat cagcgacgct ttagccagga tgtcgcgccg cagtatgtcg acatcagtgc
     7681 gggaggtgag aaatgattca gtatctgaac gtcttttttt acgatatcta cccgtacatt
     7741 tgtgcgacgg tgtttttcct cggcagctgg ctgcgctacg actacgggca gtacacctgg
     7801 cgcgcctcct caagtcagat gctcgataaa cgcgggatgg tgatatggtc gaatctgttc
     7861 catatcggca ttttggggat cttcttcggg cacctgttcg gcatgttaac gccgcactgg
     7921 atgtacgcgt ggtttttgcc agtggcagcg aaacaactaa tggcgatggt gctcggtggt
     7981 atttgcggtg tgttgacgtt gattggcggc gcaggactgc tgtggcgcag gctgacaaat
     8041 cagcgcgtgc gtgccacttc cactacgccg gatatcatca tcatgagcat tttgctgatc
     8101 cagtgtctgt tggggctaag taccataccg ttctcagcac agtatcctga tggtagcgaa
     8161 atgatgaagc tggtgggttg ggcgcaaagc attgtcactt tccgtggtgg atcgtcagag
     8221 atgcttaacg gtgtagcgtt cgtcttccgc ctgcatctgg tgttgggaat gaccatcttc
     8281 ttgctcttcc cgttcacccg actggtgcat gtgtggagcg ccccgtttga gtactttact
     8341 cgtcggtatc agattgtgcg ctcgcggcga taatttcacc ctggccggat agcccttcgc
     8401 tctccggcca cttgcaggct atgatagttc tccagtcaca ggagaacgtt aatgaaaccg
     8461 caggtttacc acgtcgatgc ttttacctct caaccatttc gcggcaattc tgccggtgtt
     8521 gttttccccg ccgataatct tagcgaagcg caaatgcagc ttatcgcccg cgaattaggt
     8581 cattcggaaa ccgcttttct gctgcacagc gacgacagcg atgtgcgcat ccgctacttt
     8641 acgccaacag ttgaagtgcc tatttgcggt cacgcgacag tagctgcgca ctatgtacgt
     8701 gcgaaggcgt taggtttagg aaattgcacg atctggcaaa cgtcactggc aggaaaacat
     8761 cgcgtgacta tcgaaaagca caacgatgat tatcgtattt cgctggaaca aggtacgccg
     8821 ggctttgagc caccgctgga aggtaaaaca cgtgcggcga tcatcaacgc gcttcatctc
     8881 actgaagacg atattctgcc aggcttgcca attcaggtgg caaccacagg acactcaaaa
     8941 gtgatgatcc cactgaaacc ggaagtggat atcgacgccc tttcgcctga ccttaatgcg
     9001 ctgaccgcta tcagcaaaca gattggctgc aatggtttct tcccgttcca gatccgccca
     9061 ggcaaaaacg aaaccgatgg tcgcatgttc tcgcctgcga ttggtattgt ggaagatccg
     9121 gtgactggca atgccaatgg cccgatgggt gcatggttgg tacatcacaa cgtattgccc
     9181 cacgatggca acgtgttgcg tgttaaaggc catcaggggc gcgcattggg gcgtgacggt
     9241 atgattgaag tgacggtgac aattcgtgat aaccaaccgg aaaaagtcac catttctggc
     9301 gcggcggtga ttttgtttca tgccgaatgg gcaattaaac tctgaaaccg ttgcctgatg
     9361 cgcttcgctt atcaggtcta caaccgagct aatgtatgcc ggataaggcg tttacgccgc
     9421 atccggcaac ctgacataaa ttattttccc gcctccgggt gcgtatcaaa cgccgccatc
     9481 accagcgcta actcatcaac ggtaaagcca tgtttcgcat catccacgcc cagaccaaac
     9541 tgttcttgca tcacagcata taacgacgcc acatccggta gatttcgctg ctccaccgcg
     9601 tgcccatttt cataatgggt aaaatgaaaa ttggtcagtg tcagcttgcc gccgtccggc
     9661 aaatggcggc acatcagcaa atgatggcga aaatgcgact gcggccagtg cgccgaccag
     9721 aaattgccca tcacataatc gctttgttgc tgctcgcaga gatcaaaacg gtacatcgac
     9781 tgccaatgct gatgatgatt aaactgcaac acccaatcat caccctcctg caacaaccga
     9841 tactctccgt gtggcgtggt ctgcacgaga tcggaaacta aacgaatcgg cgcggttagc
     9901 gtctgcccac cgaaaccgac atcagcaatc catttttccc cttccagttc aaccaacagc
     9961 aaacgatggg tgcgcggcgg taatgctggc ggatttgata acactacccg ccctaacaag
    10021 ctgcgaacgt gaaaccccag ctcgcataac acccgctcaa acacgccatt ctgctcaaaa
    10081 cagtaaccgc cacgacgggc tatcacgagt ttctcttcca gcgattgatc atcaagctgc
    10141 atttccctcg gcagcaaaac gtcgaggttt tcaaacggaa tggtgcaatt gtgtttcagg
    10201 tgcaatgcac gaagcgtatc aatattgacc gcagcagctc ccgaccagtt aatacgggca
    10261 aaatagtgat tcagaatggg cgtcatcagg cgtttccctt agtggttttc tcaacttata
    10321 acctggaata gacgccgcac cggctatttt gtgcgatcgc ggacatgcat cacgtttgca
    10381 aatcgccaat cccgttaatt tcgttactta ttgcgttacg gactacgctt aaaaaaacct
    10441 tcttcgcgag gcgataccat gagccgattc atccccatag aattacacca tgccagccgt
    10501 ctattaaatc atggcccaac tgtcatgatc accagtttcg atgagcaatc ccagcggcgt
    10561 aacattatgg ctgcagcctg gtcaatgccg gtggagtttg aacctccacg cgtggcgatt
    10621 gtggtagata aatcgacatg gaccagggag ttgattgaac gtaacggtaa atttggcatc
    10681 gttatcccgg gcgttgcagc aactaactgg acgtgggcgg tgggaagtgt gtcggggcgt
    10741 gatgaagata aatttaattg ctatggcatt ccggttgtga gaggcccggt atttggtttg
    10801 cctctggtcg aggaaaaatg tctggcgtgg atggagtgcc gcctgctacc tgcgacttct
    10861 gcgcaacaaa aatacgatac gctgtttggc gaggtggtat cagcagcggc agacgcacgg
    10921 gtatttgtcg aaggccgctg gcagtttgat gatgataagc tcaatacgtt gcatcattta
    10981 ggtgctggca cgtttgttac cagcggcaag cgagttacgg cgggttaagc attacatgct
    11041 gtaaccgggt ttctttatca aagcctccat ttggggctcg atttcggcat cccagatagc
    11101 ttgccaagat tctggttgaa tctgctgtag agaaatgctt atcgagctat ctttactgtt
    11161 cagatggcga ataataacgt cggtaatatc tgtagcaagt gctgcttttt gttgttcgtc
    11221 cagttcacgc ggaaaacatt taatgtcgat gtgcggcatg attgtgctcc ttttgaaaga
    11281 tgtcagtaaa attaacacac attgtgaatg tcgtatgtcc ggtgatgcgt ttttacgaca
    11341 ataatgccta cttgaaattg tagatatttg cactacaccc ttttaaataa agcaccaaca
    11401 aatcgtcaac ttgcagaaca tattcttctg cctacatacc attaagaaaa catcaagttc
    11461 ccgacttgcc aatttattaa cgcccattat agacgctgaa acaagccacg gacaaagaag
    11521 atgcaaaaat taccggcaat gcagaaaaac tcaacgaacc agggcaagat tacgaaagcc
    11581 cgctccccgc aaggactgac gccagatagt ttctgtccat ggctgctttt cgcatcttac
    11641 gtcttaaccc tgccttgaat accttatcat tcgtcaaaat attaatagca atgtgccgta
    11701 tccctgaaaa taattctgct gcatttcctc ttcttatttt gcagtcgtct tcattcatta
    11761 ccacgtccag acgccagtgc agcttattct ccacgtgcca gtggtttcgg attgctgtgg
    11821 cgaacttctc tgcggttaaa tcagcagaac tgatataata tctgaccgtc atttctggct
    11881 ctttcttttg ttctgctatt attgagcgaa aggtgactgc cacgcataat ttcttcagcc
    11941 ctttccattc aaacgtgaaa tcaataagtt catcagggac ttcgcaaaca atataaagac
    12001 gggtttcttc tctgccgtga ctcttttcac taattgcgta actgtcatgc tctggattat
    12061 ttaattcttt cagcggaaat ttttcctcaa aggctttatt tagccgcccc tggtttcctt
    12121 ttaccgcgaa taaataatca cctccctgtt tttgtatctt ctctgcaata tctttctggc
    12181 aacccatcgc atcagttgtg atgatttttc ctttaatatc cagcatgtta agaagttcag
    12241 ggatagctgt aatttcattg gatttctcat ccgtcctgat ctgtccgatg accagactgt
    12301 gcattgttga gaacgcacta atgacatgaa tcgctccctt gcggcgactc ttgtcataag
    12361 aatgccggag tgtttttcca tcaatcgcga tgacgtcttt gtcatttgat gaatggcagt
    12421 cacgcatc
//
LOCUS       DAPGRN010000129        12141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPGRN010000129 DAPGRN010000000
VERSION     DAPGRN010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.131"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(372..1397)
                     /locus_tag="RHW33_003948"
     CDS             complement(372..1397)
                     /locus_tag="RHW33_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1647404.1"
                     /translation="MDNPDKTPVPEVTVATEPQPSAECFTFGDPIPVLDRRELLDYVE
                     CVQIDRWYEPPVSFAGLARTYRAAVHHSSPIAVKRGILSSTFIPHRLLSQQSFARFVQ
                     DYLVFGNAYLEKRTNWLGDVLSLEPALAKYTRRGVDLESYWFVNYGLTMQPYEFTRGN
                     IFHLMEPDINQEIYGLPGYLSAIPSTLLNESATLFRRKYYINGSHVGFIMYMTDAAQN
                     QEDVNNIRKAMKSAKGPGNFRNLFMYSPNGKKDGIQIIPLSEVAAKDEFLNIKNVSRD
                     DMMAAHRVPPQMMGIMPSNVGGFGDVEKASRVFVRNELMPLQKRLQELNDWCGEKIIS
                     FEPYTLE"
     gene            complement(2386..3633)
                     /locus_tag="RHW33_003949"
     CDS             complement(2386..3633)
                     /locus_tag="RHW33_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV1647405.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVYYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKHEQIVMETPVPSAI"
     gene            complement(3705..4619)
                     /locus_tag="RHW33_003950"
     CDS             complement(3705..4619)
                     /locus_tag="RHW33_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV1647406.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRNICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4835..6268
                     /gene="cscA"
                     /locus_tag="RHW33_003951"
     CDS             4835..6268
                     /gene="cscA"
                     /locus_tag="RHW33_003951"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV1647407.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADSWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFVAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSISPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVVQHGALWQLG"
     gene            complement(6276..7334)
                     /locus_tag="RHW33_003952"
     CDS             complement(6276..7334)
                     /locus_tag="RHW33_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1647408.1"
                     /translation="MDLASDVCFCYSARFTANGLDMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGSVTRIPCPLLIRCST"
     gene            7502..7720
                     /locus_tag="RHW33_003953"
                     /pseudo
     CDS             7502..7720
                     /locus_tag="RHW33_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7738..9066
                     /gene="dsdA"
                     /locus_tag="RHW33_003954"
     CDS             7738..9066
                     /gene="dsdA"
                     /locus_tag="RHW33_003954"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV1647409.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLTQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMEQYLAKGR"
     gene            complement(9175..10713)
                     /gene="emrY"
                     /locus_tag="RHW33_003955"
     CDS             complement(9175..10713)
                     /gene="emrY"
                     /locus_tag="RHW33_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV1647410.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSNTIIILTVVSVISLISLVIWESTSENPILDLILFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10713..11876)
                     /gene="emrK"
                     /locus_tag="RHW33_003956"
     CDS             complement(10713..11876)
                     /gene="emrK"
                     /locus_tag="RHW33_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV1647411.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTRNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3403 a         2882 c         2737 g         3119 t
ORIGIN      
        1 gctatagtaa aaaaaccatc tgaggaagag ttcagtaaaa tatataagct atttgaaaat
       61 acacagttaa cgatcccgac aaaattatcc gaaactccag agccagttaa aacaacagca
      121 atgcgactca ttttgctgta tagaggaact attctttctg ggttaagatc aaattcgact
      181 gacttagctg gttttttggt tagaaatgtt tgcacgttaa tgatggtcca tggcctactc
      241 aaaacaacgc caataatgga aaatcgatat cttcgatacg agttaactga gaaaggtgcc
      301 gcatttttct catatatcga tttacgtaac cttcccgatt aaataaaaaa gccacttcag
      361 tggctttttt attactcaag tgtataaggt tcaaaactaa ttattttctc gccacaccag
      421 tcgttcagtt cctgcagtcg cttctgtagc ggcatcagtt cgttgcgaac aaaaacacgg
      481 ctggcttttt ctacatctcc aaacccccca acgttactcg gcatgattcc catcatctgc
      541 ggtggcacac ggtgcgctgc catcatgtca tcacggctca cgttcttgat gttcagaaac
      601 tcatctttcg ccgcaacttc ggataggggg atgatctgga tgccatcttt tttgccattg
      661 ggtgagtaca taaagagatt gcgaaaattg cccggtcctt tggcactttt cattgccttg
      721 cggatgttgt tcacatcctc ctgattctgc gcagcgtcgg tcatgtacat gatgaagcct
      781 acatggctgc cgttgatgta atatttccga cggaacagcg ttgcggactc gttgagcagg
      841 gttgacggaa tggcggagag ataaccgggc agcccgtaaa tctcttggtt aatatccggc
      901 tccatcagat gaaaaatgtt gcctctggtg aactcataag gctgcatcgt caggccgtag
      961 ttcacaaacc agtaggattc taggtcaacg ccgcgccggg tatactttgc cagcgctggc
     1021 tccagtgaga ggacatcacc cagccagttg gtgcgctttt ccagataagc attaccaaac
     1081 accagataat cctgaacgaa acgggcgaaa gactgctggc tgagcaagcg gtgtgggatg
     1141 aaggtgctgc tgagaatgcc acgcttaaca gcaattggtg aactgtgatg cacggcagcg
     1201 cggtaggtac gtgccagccc ggcaaaactg accggcggtt cataccagcg atcaatctgt
     1261 acgcactcca cataatcaag cagctcgcgc cggtccagta ccggaatggg atcgccaaag
     1321 gtgaagcatt ctgcagaggg ctgcggttct gttgctacag tgacctcagg tacgggagtt
     1381 ttgtccgggt tatccatatt tttctcgctg tgaacaattc ttgagtgatc aggtgtgtcg
     1441 tgaacagtac aacgatattt gcttgacggt ctgattactg cgggcgggta ttgtgtcaaa
     1501 ttcgatacaa taaagggttt tatgaatgag aatacgtcat atcacatggg ggatggtata
     1561 cacggttaaa cccggttata gcgctggatg gtgagtgatt taaagccatt ctggtataca
     1621 taaaatgaaa aaaatacttt agggtgtatc cccctgactt cccagagttg gcgctggttt
     1681 gcgagcagca ttgtataatg cgtggggtgt gataactttt ttgcgcattt ttcaaccttt
     1741 tcgcagattt gccagccccc tgccccgtcg ttttttcatc caactattca ttattgaaac
     1801 tgacggaata ggccgatctc tatggcctta atgagatgtt tttatcgttt gatggtatcc
     1861 atgatggtat caacaaaaca accacttaaa ttacctttat aaaacataga tataaataca
     1921 ttttcgattc ctgcagggac accagatacc cttcaaacca aatctacctt catcccgtaa
     1981 acgatgggtt tggcagcaca cttgccctat atctactcat ttttactgca acaggttgaa
     2041 atctcagcac tgtcagaaag cgctgatgac taaacagccc tgggccgggc gatgtaacca
     2101 tcacacagaa tcctgatagc gaaatacggc gtgactcgat acttcactcc gcaatgcatt
     2161 ccttgatgaa ttcgcaggcc cgtgatacac gggacaggtc gctgaatgac gacaatgttc
     2221 tggaaatcag cgaaccgtgt atccggagta catttgagcg actgtaccag aacatgaatg
     2281 aggtgtttgg attaggcgat tattagcagg gctaagcatt ttactattat tattttccgg
     2341 ttgagggata tagaactatc gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg
     2401 tacaggcgtt tccataacta tttgctcgtg ttttttactc aagaagaaaa tgccaaatag
     2461 caacatcagg cagacaatac ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc
     2521 aaagagtatc ccagtcggcg ttgaaagcag cacaatccca agcgaactgg caatttgaaa
     2581 accaatcaga aagatcgtcg acgacaggcg cttatcaaag tttgccacgc tgtatttgaa
     2641 gacggatata acacaaagtg gaacctcaat ggcatgtaac agcttcacta atgaaataat
     2701 ccaggggtta acgaacagcg cgcaggaaag gatacgcaac gccataatca caacaccgat
     2761 aagtaatgca ttttttggcc ctacccgatt cacaaagaac ggaataatcg ccatgcacag
     2821 cgcttcgagt accacctgga atgagttgag ataaccatac aggcgcgttc ctacatcgtg
     2881 tgattcgaat aaacctgcat aatagacagg aaaaagttgt tgatcaaaaa tgttatagaa
     2941 agaccacgtc cccacaataa atatgacgaa aacccagaag tttcgatcct tgaaaactgc
     3001 gatgaaatcc tcttttttta cccctcccgc atccgccgct acgcactggt gatccttatc
     3061 tttaaaacac atgttgatca tcataaatac agcgccaaat agcgagacca accagaagtt
     3121 gatatgggga ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag catagccgaa
     3181 agatccccat gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt
     3241 gaagctgtca agcaaaccgc atcccgccag ataccccagg ccaaaaaaga gcgcccccag
     3301 aattaaacct acagaaaaat tgctttgcag taacggttca taaacgtaaa tcataaacgg
     3361 tccggtcaag accaggatga aactcataca ccagatgagc ggtttcttca gaccgagttt
     3421 atcctgaacg atgccgtaga acatcataaa tagaatactg gtaaactggt tgaccgaata
     3481 aagtgtacct aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa
     3541 cgaccaccac agcgaccagg aaataaaaaa gagaaatgag taactggatg caaaacgata
     3601 gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt taaaaaattc
     3661 acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc cagttcttgt
     3721 cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc gcaacgttga
     3781 gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccgt agaggacaga
     3841 cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac acaattcaca
     3901 gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacccc ttctgcacct
     3961 ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat attccgatcg
     4021 ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac gacatccgcc
     4081 agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca tagatcttca
     4141 cgaatattgg gatcgaagct gacaaaacct ccggcatgcc ggatcgcagt catcgcagta
     4201 aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg taaccattcg
     4261 ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc
     4321 ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt ggatgtccgg
     4381 tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag cgttctttgc
     4441 attaacgcac caaaaggatc atcaccgacc cgacctataa acccacttat tccgcctaat
     4501 ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag taggcgcccg
     4561 tctgattctg gcaagagatc tactaccgca tcccctaaaa cccatacttt ggctgacatt
     4621 tttttccctt aaattcatct gacttacgca tagtgataaa cctctttttc gcaaaatcgt
     4681 catggattta ctaaaacatg catattcgat cacaaaatgt catagttaac gttaacattt
     4741 gtgatattca tcgcatttat gaaagtaagg gactttattt ttataaaagt taacgttaac
     4801 aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat tgcatgcggc
     4861 gcaaaacgca ctagcaaaac ttcatgagca ccggggtaac actttctatc cccattttca
     4921 cctcgcgcct cctgccgggt ggatgaacga tccaaacggc ctgatctggt ttaacgatcg
     4981 ttatcacgcg ttttatcaac atcacccaat gagcgaacac tgggggccaa tgcactgggg
     5041 acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc tagcgccagg
     5101 agacgataat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct
     5161 ctcacttatc tacaccggac acgtctggct cgatggtgca ggtaatgacg atgcaattcg
     5221 cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac agggtgtgat
     5281 cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga
     5341 ctcatggtgg atggtagtcg gggcgaaaga cccaggcaac acggggcaga tcctgcttta
     5401 tcgcggcagt tcattacgtg aatggacttt cgatcgcgta ctggcccacg ctgatgcggg
     5461 tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc attatctgat
     5521 gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct ttcaaagtgg
     5581 cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc attttactga
     5641 acttgataac gggcatgact tttatgcacc acaaagcttt gttgcgaagg atggtcggcg
     5701 tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac gtgaaggctg
     5761 ggcaggctgc atgacgctgg cgcgtgagct atcagagagc aatggcaaac tcctacaacg
     5821 cccggtacac gaagctgagt cgttacgcca gcagcatcaa tctatctctc cccgcacaat
     5881 cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc
     5941 gctgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa tgcggctgta
     6001 tattgataac caatctgagc gacttgtttt gtggcggtat tacccacacg agaatttaga
     6061 cggctaccgt agtattcccc tcccgcagcg tgacacgctc gccctaagga tatttatcga
     6121 tacatcatcc gtggaagtat ttattaacga cggggaagcg gtgatgagta gtcgaatcta
     6181 tccgcagcca gaagaacggg aactgtcgct ttatgcctcc cacggagtgg ctgtggtgca
     6241 acatggagca ctctggcaac tgggttagca taatatcagg tggaacaacg gatcaacagc
     6301 gggcaaggga tccgcgtcac acttccccct tcacgacctt caataatatg caatgcagct
     6361 tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa aaacagatgc
     6421 ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat acgtaccccc
     6481 ttcgccagga gaacctgata agccacaaag gctgcgcgat cgttaccaca tatcagaaca
     6541 tcaaaatctg gtttgcccga tttgaagtgg tcattgagta aacttgcgag atcggtgtag
     6601 tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg tccagcatca
     6661 cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact ttccggtagc
     6721 cagaagcata acggttgacg atagcccgcc gcgagcaaat gctgtgttga ttcatattgt
     6781 gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac acagttcgcc
     6841 aatacaatat tttcaccata cagagactca ggcagcgtga tatgtcgcag ccccattgta
     6901 gtatagataa tgccatccgg acggtgagca agcagctgac gtgccgcgcg ggcagcgtca
     6961 tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc ggtttgctca
     7021 atggcaagca gaatatcaac agagaaagga gtggtagcag tgtcctgcgc cagcacggcg
     7081 agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc aggaacataa
     7141 ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac agattctgca
     7201 ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc gacatccttt
     7261 aatgaagcca tatccaagcc gtttgccgta aaacgggcac tgtagcagaa acagacgtca
     7321 ctggcgagat ccaacgccct atcacctgac acggcaatac aataaaaaat aacaataatt
     7381 cccggacaat tgtccccaat tccgcctctg ttctcgccaa cgagtaacca tataggcaag
     7441 taagtaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa tatggatcat
     7501 tgtgctgccg ctgtatcccg acatcagccc ggaaattatt acgattgcta tcggttcagg
     7561 cgcgattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc aatattgcgg
     7621 cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg cttcagtcgt
     7681 cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag acgtactatg
     7741 gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga tctggttgct
     7801 cttaaagaaa ccacctggtt taatcctggc acgacctcat tggctgaagg tttaccttat
     7861 gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg ttttgcaccc
     7921 tatctggcaa aagcatttcc tgaaactgct gccactgggg ggattattga atcagaactg
     7981 gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc gatcagcggg
     8041 caactgttac tgaaaaaaga tagccatttg cccatttccg gctccataaa agcacgcggc
     8101 gggatttatg aagtcctggc acacgcagaa aaactggctc tggaagcggg gttgctgacg
     8161 cttgatgatg actacagcaa actgctttct ccggagttta aacagttctt tagccaatac
     8221 agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat gagcgcccgc
     8281 attggcttta aggtgacagt gcatatgtct gctgatgccc gggcatggaa aaaagcgaaa
     8341 ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt tgccgtcgag
     8401 gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga cgaaaattcc
     8461 cgcacgttgt tccttgggta ttccgtcgct ggccagcgtc ttaaagcgca atttgccgag
     8521 caaggtcgta tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg tggtgttggc
     8581 ggtggtcctg gtggcgtcgc attcggactt aaactggcgt ttggcgatca tgttcactgt
     8641 ttttttgccg aaccaacgca ctccccttgt atgctgttag gcgtccatac aggattacac
     8701 gatcagatct ctgttcagga tattggtatc gacaacctta ccgcagcgga tggccttgct
     8761 gttggtcgcg catcaggctt tgtcgggcgg gcaatggagc gtctgctgga tggcttctat
     8821 acccttagcg atcaaaccat gtatgacatg cttggctggc tgacgcagga agaaggtatt
     8881 cgtcttgaac cttcggcact ggcgggtatg gccgggcctc agcgcgtgtg cgcatcagta
     8941 agttaccaac agatgcacgg tttcagcgca gaacaactgc gtaatgccac tcatctggtg
     9001 tgggcgacgg gaggtggaat ggtgccggaa gaagagatgg agcaatatct ggcaaaaggc
     9061 cgctaattaa ttttcaatgc agcatcgcca tcctttccct gggtgagcga tgctgccgat
     9121 ggcgcaggct taagattcta ccgtcttacc tgctatagct ctctcttttt accatcaccc
     9181 aacacctttc gctgtaaacg gcggtttcgc aaaccaaacc aataccgtaa gtaagataaa
     9241 agcaatagcc gccatacgga aaatctcatt tgcagaaatt gaaagtgact gctgagttat
     9301 ttcattatta atttcattaa gaacccctga aagcgaacca tagtatttat ccataatttg
     9361 tgacgatgaa ttaaacacgg ggttaaattg atcgatggtt gctgtcaact gactatggtg
     9421 taatgattct cggcgtcccc acagcgtcat tgtcaacgac gtaccaactg atcctgacaa
     9481 ggtacgaaaa aaattactca tactcgaggc attggcaaat ttattatctg gcaaacctga
     9541 aaacgaaatc gttgttaagg gtaaaaagaa acaggcgacg gcgaatccct gaaaaaactg
     9601 tggcaaaatg atgcctgtaa aatcaatcgt tggcataaat gtcacagaac gccagtaata
     9661 gcaaaccgca tacattaaaa aactaaatgt caccaacaac cgcatatcaa ttttgttgcc
     9721 ataacgtcct atcaaaggtg atattaatag cggcatgatg ccgatgggcg cataggcaag
     9781 tccggcccat atcgcattat accccatcgt ttcctggagt aactgcggca taaggactat
     9841 cgccccagag taaaataaat atgcgcatgt gatactcaca ataccaatgg tgaaattacg
     9901 ggacttaaac aaaatgagat caagaatcgg gttctctgag gtcgactccc aaatgactaa
     9961 agagatcaga gaaataactg atactactgt taatattatt attgtattcg agttgaacca
    10021 atccagatcg cgccctttat caagcataat ttgcaagcca ccaacaccga gcactaacag
    10081 ggtcagtcct ggtagattca ttttgaccgg tgaagtctca gtttctcttc ctttaagtaa
    10141 ggttaagcat aatgtcagga cgataatacc catagggaca ttgattaaaa atatccaacc
    10201 ccagctaaag ttatcgcaaa tatatccgcc taatatcggc ccacatatcg gagcgataat
    10261 cacggtcatt gaccataatg ccagagcaaa tgttcttttt tctggtggat aattccttaa
    10321 taataaactc tgtgacagtg gaattaacgg ccctgccatt aacccctgaa cgactctaaa
    10381 aaatatcagc acatcaagat tggtcgataa actacacatt aatgaagaca gcgaaaaaaa
    10441 agtgactgaa agtaaaaata accttaattc gcctattctt tgtgccagcc tgccggtaac
    10501 agggatcgca atggcatttg ctacgccaaa cgaggtgata acccaggtgc cttcgtctgt
    10561 tgatgctccc agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa
    10621 catttgcata aacgtcgcta atgacaatgc aatagtgacg caccataacg tcccaccggt
    10681 taatggtgcc ggagttgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg
    10741 ttgctaattt ctttttctat cggactggta tcgataacta aagccttact ggtataagcc
    10801 ggcatggagg tcacggttga agccagatca ggcatctcag caatgtcttc gttcctggta
    10861 tcaatagttg cagtcatcga taaaccaatg cgcaaggggt gttccatgag ttcttttgga
    10921 tccagagaaa cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt
    10981 tgtgcaggta ataaggagaa cgcattgccg gttcccatat tgatccctgt cacccggcca
    11041 tgaaacacaa cattctcacc ataaagatcg ctgataatat tgaccgattg accaatccgt
    11101 acatccgtga gttgtgtttc tttaaagttg gcattaaccc acatttgacg tgctggtacg
    11161 acagccatta acgattgtcc gggactcact gtttcgccga cctgaacact tcgctgggca
    11221 atatagccgg taaccggact cttaatatcc gtacgtttaa gcgccaacca ggcttctttg
    11281 gttgcatccg cagcttcaac gacttgtggc tgacggttta atggtgtgtt cattactaaa
    11341 gctttattcg ctttataagc ctggatagcg gcattcaatg ccgctttgct acttattaac
    11401 gtatctttgg tatgctccag cgtttctttt gaaataaccc cctgcttcgc taaaggcaca
    11461 cgacggttat aatcttctaa agattgttga tactgaatac gtgctgaagc gacttcggca
    11521 ctgtattgtt tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt tgccagatta
    11581 tttttagctt tattgagtgc gatagtggca tcagttttat ccagtgaaac taaaatgtca
    11641 ccctgtcgaa cgtagttcgt atctttatga ttaactacag tgacactacc tgagacttgt
    11701 gcagaaattg gatctgcatt ccccgtgaca taggcgtcat ctgtactaat catgtcttct
    11761 aattccattg accaataggc ataagcacct gaaaacgcaa taaataaaac tatcgccaat
    11821 aaagaaaagt attttcttct gttagaatgt tttttatttg aattaatctg ttccactatt
    11881 atctctcatt tcgcatagat gaaatttatg aattgaatga aaagagtatt cattcttcat
    11941 atcaaacaca gtgcatgtgt tgcacattat cccaggaacg ttggaggcct aatttaaatg
    12001 aggtgcaaaa aaaacaacag aggactaaac cgtggctttt gcaatacaat tcttacgcct
    12061 gtaggattag tgagaagact tatagtgcta acttgaaact ataaatcatc ggtataattc
    12121 ctgattttat tgttgacatt t
//
LOCUS       DAPGRN010000130        12096 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPGRN010000130 DAPGRN010000000
VERSION     DAPGRN010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.132"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            102..659
                     /locus_tag="RHW33_003957"
     CDS             102..659
                     /locus_tag="RHW33_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV1647412.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            complement(951..1970)
                     /locus_tag="RHW33_003958"
     CDS             complement(951..1970)
                     /locus_tag="RHW33_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HDV1647413.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRDDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTRKIKEVPQAANVQAPRETSIITFNNS
                     INPFK"
     gene            complement(1963..3672)
                     /locus_tag="RHW33_003959"
     CDS             complement(1963..3672)
                     /locus_tag="RHW33_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV1647414.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHHGNDSDYDAAQALCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPGFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            3748..10515
                     /locus_tag="RHW33_003960"
     CDS             3748..10515
                     /locus_tag="RHW33_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV1647415.1"
                     /translation="MNKLSIGVFRCSSVSEILKYIRAITSHRAPIRYGVEKVEGKSYD
                     RLRREANQKAIDLLNSLVDGATLTDEQRQILAGYTGEGGIGGSVSEYYTPKPIAEGVW
                     EIMKLYGADVGNTLEPSAGTGVFNETKPVGTVMTATEISSVSGRINQLLHPEDSVQIS
                     PFEQLAVSTPNDSFDHVVGNVPFGGRDNTRNIDKPYAEETDMGSYFMLRMLDKIKPGG
                     FMCVIVPPSIVSGSNMKRLRLRLSRKAEFLGAHRLPTGTFDANGTSTVVDVVLMRKHP
                     AEMAEKIPLVDESTLESANVLWSTFISGKWFEKDGRRFVHGTQEKGFQGRIEVRADGQ
                     IDNQALKAKLIHRFESRIDWSLLDMAEPSPTADVVGEGEMRLINGVWQKYAGGRWIEA
                     DAGKELKIDAASYGADSWEALQRNLTTTEGRLGMTFTQMANVRDKYTTSISDDMVQLV
                     DWINSQPEKYRERLYRGAMIGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRF
                     GNPGRGPIAKLSGSGARAWFAFRGAIKLDGAISDELTGKLVTHDSSASYDSTSYQDTL
                     RYLYSDLTRDPIQLDDFRLAFTGELPASDDELLNLLASTPGIAVSPYGGIVPFARATS
                     GDINEIVAPKQEFLATLPDGPVKNNVLNQLAAIEEKRIKTPAENIRFKLNSRWFDRSV
                     ILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGSDGLFVGHRYGVVQRKDKETGEIRY
                     EWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYREQMALLEDQFNKWIKTHDRY
                     DELVTKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDYQNSEVRRLSEDGRGILGFGT
                     GLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYYEAKEFLSEEAFSNYLFVGLD
                     VLMDGDQIRQVPVLDENGKPVLGTDGTPVMRDALKLADEATITARMNAIPHSNYRAVV
                     FTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMDSDSHRKEAARRRVLSEYSDT
                     GTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREASQLAYLPTSAVAQSARDMAIK
                     NAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPKEYWQNMGIYGPDDFVKFFGK
                     TRLETVQKISGEVEEKMALVGFENLDALRGIFHRWVTLKTAEDVKDTVEIPELDEHQQ
                     DAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGVIEHEKARPIFSIIRDMDRVC
                     TDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDDDSDDSITQQSQYSLIDKGEF
                     IQLQVPEAFEQEVNKRLARFGIDEQTVTHPVTPKYAKLIATLKEFFPEGKQIIFTDEK
                     TQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKKLKAVKPPKELPDEPTDAQIA
                     KYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPIIICNKKAEVGINLHRGTTDI
                     HHLTLPWTPASIAQRNGRGARVGSNRASVRVHYYCGKGSFDEYRLKTLKRKAGWISDI
                     LRSDKSEMENADANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAQRARQKEQATIDLQ
                     NYIKAQHAAGEDVEVLTAELERSKAELEKTTAEVAKFKQAAMAKAADNADWKARWGSV
                     HHTDRMLLAQYRASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDALN
                     KGILDVDPDLVNHASEFLVIGDRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTF
                     KGTKSGNWDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIVSCDDFYRFQQRGMIK
                     ITDSYGVQTTESGYSIDFVGTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQ
                     IRSAEAFLVELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSE
                     LEVTNAIFGTNEFRASDYEITTAQFGTIGIYSNKDEIKQAMDAASARIAAERKVNLNH
                     AVAALTQSWVTAIREAATTGKITPAIADVVNDGSKFMDAYKMDAVQLPSAYGQLSYRM
                     TYNLVSMFTDLAILGLVDLNEVTPELLSMRKNHVEILHRINTVLAGRTDEEKQADADR
                     INLALGNITEEEIAARNEKQEELSSIQGDATSIAQSLGLNYRVSTADLKMMYAPKFAA
                     GEVFGLQEASGMKGGLFRAKDAIKAKFGARWLPAKAKNSDFPGNWWIIETKHNVADVL
                     AVIQQYA"
     gene            10549..10989
                     /locus_tag="RHW33_003961"
     CDS             10549..10989
                     /locus_tag="RHW33_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDV1647416.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            10986..11234
                     /locus_tag="RHW33_003962"
     CDS             10986..11234
                     /locus_tag="RHW33_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HDV1647417.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDREFEEWEQIIIDNALLLNAD"
     gene            11365..>12096
                     /locus_tag="RHW33_003963"
     CDS             11365..>12096
                     /locus_tag="RHW33_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDV1647418.1"
                     /translation="MIWFKMDMKKETIFSEVETANSKQLAVLKANFPQCFDKNGAFIQ
                     EKLLEIIRASEVELSKESYSLNWLGKSYARLLANLPPKTLLAEDKTHNQQEENKNSQN
                     LLIKGDNLEVLKHMVNAYAEKVNMIYIDPPYNTGKDGFVYNDDRKFTPEQLSELAGIE
                     LDEANRILEFTTKGSSSHSAWLTFIYPRLYIARELLKEDGVIFISIDDNEDKQLGLLC
                     DEVFGQGNFVAKLPTIMNLKGNHDNF"
BASE COUNT         3126 a         2932 c         3255 g         2783 t
ORIGIN      
        1 atttgcatta tcctgtgcct tctaaaggga tcgagtcagt cggtattggc tgtgaatggg
       61 tgtttgtcct ggagcgtaaa aaattcgctt atgaggtctt tatgaaggga aaaacagccg
      121 caggaggcgg tgcaatttgc gctattgcgg tgatgattac catcgtgatg ggtaatggca
      181 atgtgcgaac caaccaggcg gggcttgagc tgattggtaa cgctgaaggt tgccgacgtg
      241 atccatacat gtgcccggcg ggggtatgga ctgacgggat cggtaataca cacggagtaa
      301 cgccgggtgt gcgaaaaacc gaccagcaaa tcgccgctga ttgggaaaag aatatcctga
      361 tcgctgaacg ctgtattaat cagcactttc ggggcaaaga catgcccgat aatgccttca
      421 gtgcaatgac aagcgcggca ttcaatatgg gatgcaatag cttacggacc tactacagca
      481 aagcgcgagg catgcgagtc gaaacgtcca tccacaagtg ggcgcagaaa ggggaatggg
      541 taaatatgtg taaccatctc cctgattttg tgaacagtaa cggcgtgccc ctgcgaggtt
      601 taaagattcg ccgtgaaaaa gaacgccagc tttgcctgac ggggctgatc aatgaataaa
      661 ctccggcagc tccgccgact ttcgacaatg aagttatcgc tggcggcgat agttttcgac
      721 tcgattttca tggcggtata tgtgctcaat gagacgtggc cactggaacc gctattgtat
      781 gccgggcttc ggctgtgcct gacatttttg agcatggctg cgagattgat gcagcagaaa
      841 gaaacagctt cagattgtcc aggccgcgcg gtgcgcaaat atatggcacg caggcgaagg
      901 tgataatagt taacgagaac cccggcagct gccggggtta tttttggtgg ttatttgaac
      961 ggattgattg aattattaaa cgtgatgatg cttgtctcac gcggtgcctg gacgttagcc
     1021 gcttgcggaa cctccttaat tttcctggtg acaggcaagt tgcgtgcgcc aactttgatc
     1081 agagattcga aaagtgtggc aacgattttt gcatcaccag gttctttgag gcggaatgcg
     1141 tctttttggg cggcggagac gaagatcggg aggttatcca gttcgtcttg cattgctgcc
     1201 agcacatcgt cgcggatacc cgctgtttcc tccagcaaag cgattcgcgc ttcagcatct
     1261 gcgatcttgg ccattgcttc gaggtggcgg ccctggcttt cgagtagtgc ggtttccagt
     1321 tctgccgtac gctctgtcgc ctccaccatc atttccagtt cagccatttt gccgtaatgg
     1381 gatataactg cctgcactga ctcgtcggag tacccatgcg ccgccaggga ctctgccagt
     1441 agagatttag aatccgcgct ttcaaacatt ccggcgctgg caggatgatc cagactgata
     1501 tagttcggcg ttgtcacata atccacacca tggaagctgg tggttacagc gattttcccg
     1561 gactcgcgcc cgccagtggc ccagctccag ccaccagctc ggctttcgat catcgcggcg
     1621 acaattttac ccggctctgt gttaagaatt tcctgtgtat gggtaacgat gccgttgtcg
     1681 tcaacagata tagccactgt gcgacacgcc ggaacattgt cgattacgac cgggcgacct
     1741 tccaccatga tcacgctggt ttctggtact tccagtttgc cagtcagctg tcggcgaccg
     1801 tgaccgtaat agccgaaaag ctcaccaagg cgtaaacctt cctgagtttc cttgctttca
     1861 agcatggtct ttaccgcgct taatacatac tgtcgcccgt tctggcgacc ttttcgagca
     1921 ttgctataga gacaaaagcg gtcagtgacc gttttcaaaa catcagtcat tatcgtttcc
     1981 ctctttaaag accgattcaa ggatttgcgc cagttcctgt ggcggtgttt tgatgatgga
     2041 atccatcagg tgatcgtcgt cctcgctttt cgctttcagt tcgttcacca gtgcttcaga
     2101 gattttttcg tcaatctcca gcacatcgct gaacaggtaa cgtttgaatg catcggaatt
     2161 ggcgaggacg ctgttattgc tgacggcatc gaggatttgc gtaacgatgg tggcgtagtt
     2221 cgcctgcgag tcgcggttgt cgttgtgctc ttgttgcaga gcggtattaa cggagtggaa
     2281 ttcgattttg tacgggcgat caccttccgg gtataccttg ccgtacttga aagcaagatg
     2341 aatatcgata gcccgctgaa tgaactcttc tacgccctgc tggatccatg aggcgcgcat
     2401 ggcggcctga attgccgtgc gcaggaatcc accttcacca agcccgccgg acatttgatc
     2461 tgcccacccc aggagggtgt aatcgaggcc aagtgctgcc gccagctggc gcatataggt
     2521 gagaatgtct tcaatgccgt tgatgtcagc ctggatggtc tgagtatcaa tagtcatctg
     2581 tcccttgccg tcgcccataa taggcagcag agtattggtc accgtaggca tgttattcgc
     2641 gccgcgtgcg cgcttttcca tcaggtcagc tgctcgttta agcgtctgag taatggtgcg
     2701 tgaataatcg gctgctttta ccggatccag actattcatc gccaggccga tgattcggtc
     2761 aattttcgac gcgttaaaac gcgttgcctt cagtgagcgg atcgccgaac gcagattcat
     2821 gtacggctcg taggcgtatt cgagcaagct ggtcccgtaa ttctgggttt caatcggtgt
     2881 gcgctcttcc ggattatcca gcaggctgta agccttatgg ccagtgtgca caggcataag
     2941 gtttgactta ggccgccagt aggggatttt catagggata atggcccacg gatcggcgaa
     3001 aaccattttc cctgacgcgt ccttcagata atcgccgcta aatcccgcca ggttgccgct
     3061 gacctcgaac tctttgatga agcccggaag ggtgtaatag gagcactcaa aagacgtgat
     3121 ccctatgcct tctttggcgt atggcctgac ataagccacc ccaaatacag acatgataaa
     3181 tgcccacccg gcgacctctt tgttgatggt tcgcccgatg tcgttcatca gctcgtcaca
     3241 caacgcctgc gcggcgtcat agtcactatc gtttccgtga tgtaccggca cgatagagaa
     3301 agtttgcccg gtcttcttat cgaaagagag cgcgtgcgta atatggatgt tcagcgcggt
     3361 ggcgatcgtg ctgtaaaccg ccatctcttc gagtagcgga tagcgttgca agcggtcttc
     3421 cggcagttga acttcatcaa agataaagcg acttccgtcc accagcccat cgccagccat
     3481 gccactatcg cccggtttgc cgcctaagaa gccggacagt tgtaccggtg cccctgcgcg
     3541 agaaaacaaa tacccacttc cgccgtgcac agccagcgcg gacaggagga tgttgtcccg
     3601 ttctccgttg tctttaaaaa cccccgccag cgccttcctg accgaggata gcgtgatttt
     3661 attgtctgcc aagattgcac cttaattaga ataattcgca tcgtgtttga acggaattta
     3721 acactagtca cttgttaagg attaccaatg aacaagctat ctataggggt gtttcgctgt
     3781 tcaagtgtca gcgaaatatt gaaatacatt agggcaataa catctcaccg agcgccgatt
     3841 agatacggcg tggaaaaggt ggaaggcaaa agctatgacc gactacgccg ggaggcgaat
     3901 cagaaggcga tagatttgct taattcgctg gtggacggcg cgacactgac agatgaacag
     3961 cgccagatcc tggctgggta caccggcgaa ggcggcattg gcgggtccgt ctccgaatat
     4021 tacacaccaa agccgatcgc tgaaggtgtc tgggagatca tgaagctcta cggcgcggac
     4081 gtaggtaaca ctctggaacc atcggcggga accggcgttt ttaatgagac aaaaccggtt
     4141 ggtacggtga tgaccgcgac tgagatcagc agtgtttccg gtcgtataaa ccagctgttg
     4201 cacccggaag acagcgtaca gatttctccg ttcgaacaac tggctgtaag cacgcctaac
     4261 gattcattcg accatgttgt gggtaacgtt ccgttcggcg gtcgtgataa cacacgcaac
     4321 atcgataagc cttacgcaga agaaacggac atgggttctt acttcatgct ccgcatgctg
     4381 gacaagataa agccaggcgg attcatgtgc gtgattgtgc cgccgtccat tgtttcaggt
     4441 tcaaacatga agcggttacg cctgcgccta tcacggaaag ctgaatttct tggtgcccac
     4501 cgcttgccta ccggtacttt tgacgctaac gggaccagta cggtcgtaga tgtggtgctg
     4561 atgcgcaaac atccggcaga gatggctgag aaaatccccc tggtggatga aagcactctc
     4621 gaatcggcaa atgtgctttg gtcaacgttt atttctggca agtggtttga aaaggatggc
     4681 cgccggtttg ttcatggcac ccaggaaaag ggcttccagg ggcgtattga ggttcgtgcc
     4741 gacggtcaga ttgataacca ggctcttaaa gcgaagctga ttcatcgttt cgaaagccgt
     4801 atcgactggt ctttgctcga tatggctgaa ccgtcaccga ccgcagacgt tgttggtgaa
     4861 ggggaaatgc gcctgattaa tggcgtatgg caaaaatatg ctggtggtcg ctggattgaa
     4921 gctgatgcag ggaaggaact taagatcgat gctgccagtt atggcgcgga tagctgggag
     4981 gctcttcagc gtaacctgac tacaacagaa ggccgtctcg gcatgacatt tacccagatg
     5041 gcaaatgtcc gcgataagta caccacatca atcagcgacg atatggtgca gctggtggac
     5101 tggattaaca gccagcctga aaaataccgt gaacgcttgt atcgcggggc gatgattggc
     5161 cggatgttaa ttgaatatca ggacatgaag gccgccgggc atagtgctga acaaatcgaa
     5221 cagcagcgcc tttctctggt atcccgtttg caggcagaga ttgaccgttt tggtaacccc
     5281 ggtcgcggtc ctatagcgaa attatcagga agcggtgcgc gcgcctggtt tgctttccgt
     5341 ggtgcaatta agctggatgg cgctatttct gacgagctga caggaaaact ggttacgcat
     5401 gattccagcg ccagttatga ctccaccagc tatcaggaca ccctgcgtta tctctacagt
     5461 gatctcactc gcgatccaat ccagctcgat gatttccgcc ttgcgtttac cggtgaactg
     5521 ccagccagtg atgacgagtt gcttaattta ttggccagca cccctggcat tgcggtttca
     5581 ccgtatggcg ggattgttcc gttcgcccgc gccaccagcg gcgacattaa cgagatagtg
     5641 gctccaaaac aggaattcct tgccacactc cccgacggtc cagtaaagaa caacgtcctt
     5701 aatcagctgg cagcgatcga agagaagcgc atcaagacgc cagcagagaa tatccgcttt
     5761 aagctcaata gccgttggtt cgaccgttcc gtcattctgg aatttttgca ggaaaacggc
     5821 tatccggatc tgcgctatgt gcagtcagtg cagctggaag gcgacgaaat ggtttctgac
     5881 acctatcacg gtagtgatgg cctgttcgtc gggcaccgat acggtgtcgt ccagcgcaag
     5941 gataaagaaa caggcgagat ccgctacgag tgggaccgta aatcaggtga aaacgcgacc
     6001 gggttcccgg cacagctgga aaagtatctc aatggtgcgc gtatcggtgg caaagatagc
     6061 gcgacggcga acggctaccg cgagcagatg gcactgcttg aggaccagtt caataagtgg
     6121 atcaagacgc acgatcgcta cgatgaactg gttaccaaat acaacgatgt gttcaatagc
     6181 aatatcccgt atgaacactc tggcgatccg cttgggttga agggattaag cggtaagcgc
     6241 cagccatttg attaccagaa tagcgaggtg cgccgactgt ccgaagatgg gcgcggcatc
     6301 ctgggcttcg gcaccgggct gggtaaaacc acgaccgcgc tggcgcttga ggcgttcaac
     6361 tatgagaacg gtcgctccac ccgtactgcg tatgtagtgc ctaaatcagt gctggaaaac
     6421 tggtattacg aagcaaaaga attcctgagt gaagaggcat tcagtaacta cctgttcgtc
     6481 ggtcttgatg tgctgatgga tggcgatcag attcgccagg tgccggtgct cgatgagaac
     6541 ggtaaacctg ttcttggtac tgatggcact cccgttatgc gcgatgctct taagctggca
     6601 gatgaagcca ctatcacggc gcggatgaac gcgatcccgc actcaaatta ccgtgcagtc
     6661 gtgtttacca aagaacaata cgcccgcatt ccgctacgtg atgacaccgt agatgagcat
     6721 gcacaagaca tgctttatga cttcgttgcc gccgggcgcg tagccagcgc aatggactcc
     6781 gactcccacc gcaaagaggc ggcgcgtcgc cgggtattgt cggagtattc agataccggt
     6841 accgaaaaag cagagaagta tccgtacttt gaggatatgg gcttcgacag cgtgattgct
     6901 gacgaaggcc acaactaccg caatagctat aaaaatggtc gcgaagcgtc acaactggcc
     6961 tatctgccca ccagcgcggt ggcgcaatcg gcgcgggata tggcaattaa aaacgcgtac
     7021 ctgatgaaaa agaatggcgg gcgcgggccg gttctcctga ctgcaacgcc agtcgttaac
     7081 accccgatcg atgcatacaa catgctttct catgttctgc cgaaggaata ctggcagaac
     7141 atggggatct acggtcctga tgacttcgtt aaattcttcg gcaagaccag gctggaaacg
     7201 gtgcagaaaa tcagcggtga agttgaagaa aaaatggcgc tggtgggctt tgaaaacctt
     7261 gatgcgctgc gcggtatatt ccatcgctgg gtaacgctta aaacggcgga agacgttaag
     7321 gataccgtgg agatcccgga gctggacgaa caccagcagg atgcaccact tactgaagaa
     7381 caactggcgg cgtatgaaga attgcgtcag caggcggaag cggcggcaaa agccaacaat
     7441 ggcgttacga cctcggtcaa tgaagacggc gtgattgagc acgagaaagc ccgtccgatc
     7501 ttctcaataa tcagggatat ggaccgcgta tgtactgaca tggacctgta ctatcgccgg
     7561 atcacctatc gtttcctgcc ggagtacgcc gatgcggtgc agcagctggc ggacagtttg
     7621 cctaaacaag ccaccagcga agacgacgac agtgatgatt caatcacgca gcaatcgcaa
     7681 tactccctga tagataaggg cgagtttatt cagttgcagg ttccggaagc gttcgagcag
     7741 gaagtgaata agcgcctggc caggtttggc attgacgaac agaccgtaac tcaccccgtt
     7801 acgccgaaat acgcgaagct gatcgccacg ctgaaggagt ttttcccgga aggtaagcaa
     7861 atcatcttca ccgatgaaaa aacgcagcac cagaagctca agcgcattat ctgcaatgct
     7921 ctaaaccttg aaccttcaaa ggtggggatc ctgaatgctc agacggttgc cgaggcaggt
     7981 aaaaccggta agaaactgaa agcggttaaa ccgccgaaag agttaccgga tgaaccaaca
     8041 gatgcacaga tagcgaaata caacgagcaa atggctctgt atgacgccta tatcgcgcag
     8101 caaaatgaaa tgtcgttggg cggtctggaa aagattgcag ccgacttcca ggagggccgg
     8161 actccgatca tcatctgcaa caaaaaggca gaggtgggta tcaacctgca tcgaggaacg
     8221 actgacatcc atcatctgac gttgccatgg actccagcca gtattgcgca acgaaacggt
     8281 cgcggtgccc gagttggctc caaccgtgca agcgttcgcg ttcactacta ctgcggcaag
     8341 ggttctttcg atgaataccg actgaagacg ctgaagcgta aagcaggctg gatctccgat
     8401 atcctccgtt cagataagtc agaaatggag aacgccgacg ccaacgatat gatcgaaatg
     8461 cagatgtata ccgctaagga tgatggcgaa cgtctggcaa tgatgcaggt tcaaatggat
     8521 aaggcgaaag ctgcgcaacg cgctcgccag aaagaacagg ctactatcga ccttcagaac
     8581 tacatcaagg cgcagcacgc agctggtgag gatgtggagg tgcttaccgc tgaattagag
     8641 cgaagcaaag cggaacttga aaagaccacc gccgaggtag ctaaattcaa acaggcggca
     8701 atggccaagg cagcggataa cgcagactgg aaggcacgct gggggagtgt ccatcacaca
     8761 gaccgtatgt tgttagcaca gtatcgcgcg tcgttgaaaa gcgccattca gcgcaaggct
     8821 aatatctctc aagcaatctc ccgctatgag aaattattga accgtactca gaaggccgcg
     8881 acggatatca aacgcctgcg cccgctggtg gaggatgcat taaataaagg cattctggat
     8941 gttgatcctg acctggttaa ccatgcgagt gagttccttg ttatcggcga tcgctcatgg
     9001 cgtgtaggcc aatactacga ttgtgccggt gatatcgttc gcattaagtc gctggacttc
     9061 gacagccagc gcgcagacgt ggagatcatc ttcaccttca aaggcaccaa atctggtaac
     9121 tgggatgtga agacgctgga taaacaggtt gatgtaactc ccgatgaaga tgctgttatg
     9181 cagaaaatca gtggtggcgt ctccatcgcc gggattaacg acatcgtttc ctgtgacgat
     9241 ttctaccgtt tccagcagcg cggcatgatc aaaatcactg actcatacgg cgttcagact
     9301 acagagtcag gctatagcat tgattttgtt ggtacctata cggacccact gaagcatgcg
     9361 gtttacccgg atcgccgtga cggcgcgctg aagtcgtcaa ttgcaaaatg ggtgcttggt
     9421 atgatgtcgg aagggaataa ccgccagatc cgttcggcag aagcattcct ggttgaactg
     9481 tttggctcca attatggcga tgtaatcgcg tcatacggag atacgctatc ccctgaagca
     9541 attcaggaga aaatagcgga tgcgatcgcc agaatgccgg agaaaacaag ccagggggct
     9601 actcgtaacg gggattctga acttgaagtc accaatgcca ttttcggtac caatgagttc
     9661 cgggcgtcag attatgagat caccacagca cagtttggca ccattggcat ttacagcaat
     9721 aaagacgaga tcaagcaggc aatggacgca gcaagcgcgc gcattgcagc agaacggaaa
     9781 gtcaatctga atcatgcagt cgccgcgctg actcaatcgt gggtaacagc aatcagggag
     9841 gccgccacca caggaaaaat cacaccggca attgcggatg tcgtaaacga cggctctaaa
     9901 tttatggatg cctataaaat ggatgcggtg cagttgccat cagcctatgg gcaactcagc
     9961 tatcgcatga cctacaacct ggtatcaatg tttaccgacc ttgccatcct tgggctggtg
    10021 gaccttaacg aggttacgcc ggaattgctc agcatgcgca agaatcatgt ggagatattg
    10081 cacagaatta acacggtact tgccgggcgc accgatgaag agaaacaggc agacgctgat
    10141 cggataaacc tggcccttgg caacatcacg gaggaagaaa ttgccgccag aaacgagaaa
    10201 caagaagagt tatcatcaat acagggtgat gccaccagca tagctcagtc tcttggtctg
    10261 aattatcgcg tatccaccgc cgacctgaag atgatgtacg caccaaaatt cgccgctggc
    10321 gaggtatttg ggcttcagga agcctccggc atgaaagggg gtcttttccg tgcgaaagac
    10381 gcaatcaagg cgaaattcgg cgctcgctgg ttgccagcga aggcgaagaa cagcgacttc
    10441 ccgggtaact ggtggattat cgagacaaaa cacaacgtgg cggacgttct ggccgtcatc
    10501 caacaatacg cataacagga gcgcccggtt cgccgggcgt cgcataatat ggccacacta
    10561 tctgatacaa taaaaccgaa taaaacatat cttgaggcgg tactccgtac agcgttgtta
    10621 ggaaagacag aagacgaata cgttgatttc ttcctgtcag ggctacgcgg gcgattactg
    10681 aaaaatcccc gcctgtaccg cagctatggt ccatactggc cggaaattaa aaaattatta
    10741 ctggagcgcg gttatggtaa tttcggtcgt ctcgttgacc gtgacgttcg caaaatttac
    10801 cgttatgacc gcccggcgct aacactcata gccgcgacgc tctacagcca ggagcgtttt
    10861 gataatggtc agatatactc agcctggcat ttactgccag tgcctgaaga agttgacgac
    10921 caggactatg agtttgagtc ttacgatttg gaagttgaag ccttggcaca ggctggagag
    10981 aaaacttgaa aaagcgatac tacacagtaa agcatgggac gctacgagca ttacaagagt
    11041 ttgctgacaa gcataacgtt gaggtgcgca gggaaggggg aagtaaagct ctgcgcatgt
    11101 accgtccgga cgggaaatgg cgtacagtcg tcgatttcaa aacaaacagt gttccccagg
    11161 gcgtccgcga ccgggaattc gaagaatggg agcagatcat catagataat gcattgcttc
    11221 tcaatgcgga ttaaacttcc ccaaattatg gctgtttgct caccgagcat cgctcaaaga
    11281 agcacgattc ttcaaacata tagatagtga tagtgccaca acttctggct ctaacgggct
    11341 ggggaggcgg cgctttgttg ctaaatgatc tggtttaaaa tggatatgaa aaaagaaacg
    11401 attttttccg aagtagaaac ggctaacagc aagcaactgg ctgtgttgaa agctaatttc
    11461 ccacagtgtt ttgataaaaa cggagccttc attcaagaaa aattgcttga gattattaga
    11521 gcatcggagg ttgaactctc taaagaatca tacagtttaa actggctggg taaatcttat
    11581 gcccgtttgt tggccaatct accaccgaaa acgttgttgg cagaagataa aacacataac
    11641 caacaagaag agaacaagaa cagtcaaaac ctgttaatca aaggggataa cctcgaagta
    11701 ttgaagcata tggttaatgc ttatgctgaa aaggtgaata tgatttacat tgacccacca
    11761 tacaatacgg gtaaagatgg attcgtctat aacgatgatc gcaagtttac accagaacag
    11821 ctttctgaac tggcaggtat cgagcttgat gaggctaacc gcatacttga atttaccacc
    11881 aagggttcaa gcagtcatag tgcatggctg acattcattt atcctcgtct ttatattgca
    11941 cgtgaacttt taaaagagga tggagtaata ttcatctcca tagacgataa cgaagataaa
    12001 cagttgggat tattatgcga cgaagtattt gggcaaggta attttgttgc aaaactccct
    12061 acaatcatga atctcaaagg caatcatgat aacttt
//
LOCUS       DAPGRN010000131        11651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPGRN010000131 DAPGRN010000000
VERSION     DAPGRN010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.133"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            319..2742
                     /gene="pgaA"
                     /locus_tag="RHW33_003964"
     CDS             319..2742
                     /gene="pgaA"
                     /locus_tag="RHW33_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV1647419.1"
                     /translation="MYSSSRKRCPQTKWALKILTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALLWFAQKSALSNNQIADWLQIALWAGQDKQVITVYNRYRYQQLPSRGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQIDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWAQYWVASAYLKDHQPKKAQS
                     IMTELFYHKEIIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTSRELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQEPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLNDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERFFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSIE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2751..4769
                     /gene="pgaB"
                     /locus_tag="RHW33_003965"
     CDS             2751..4769
                     /gene="pgaB"
                     /locus_tag="RHW33_003965"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV1647420.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMID
                     REYFATWQQVREVARSPLIEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQKIITVQEKSPQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAIAAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVTQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKSIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNKPEIDVIRPEFSTAWYPKND"
     gene            4762..6087
                     /gene="pgaC"
                     /locus_tag="RHW33_003966"
     CDS             4762..6087
                     /gene="pgaC"
                     /locus_tag="RHW33_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV1647421.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHTALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6089..6502
                     /gene="pgaD"
                     /locus_tag="RHW33_003967"
     CDS             6089..6502
                     /gene="pgaD"
                     /locus_tag="RHW33_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV1647422.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6552..7340)
                     /gene="phoH"
                     /locus_tag="RHW33_003968"
     CDS             complement(6552..7340)
                     /gene="phoH"
                     /locus_tag="RHW33_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV1647423.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7961..9232)
                     /gene="efeB"
                     /locus_tag="RHW33_003969"
     CDS             complement(7961..9232)
                     /gene="efeB"
                     /locus_tag="RHW33_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV1647424.1"
                     /translation="MQYEDENGVNELSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERFFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9238..10365)
                     /gene="efeO"
                     /locus_tag="RHW33_003970"
     CDS             complement(9238..10365)
                     /gene="efeO"
                     /locus_tag="RHW33_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV1647425.1"
                     /translation="MTINFRRNALQLSVATLFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTEADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10423..11253)
                     /locus_tag="RHW33_003971"
     CDS             complement(10423..11253)
                     /locus_tag="RHW33_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV1647426.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSEVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLILALVAFALPPRAGATASRSA
                     "
BASE COUNT         3074 a         2860 c         2718 g         2999 t
ORIGIN      
        1 tttcatcagg actttcgaaa aatccgaaat catgcatcgg aatttactga tttaattatt
       61 ttactcctaa tttattttga aaaaggcatt gggatttatg ccgtattcct gaagatcctc
      121 atcattggaa tggattttcg ggcgagaaaa ggattttata tggacactct gctcatcatt
      181 tcttcttctc atcatcaaca attcacgtct ctcttccgcg tttaataacg gattatgagg
      241 tgcaaaaaat atctttcttt tcagttacat gtcattagat acagagagag attttggcaa
      301 tacatggagt aatacaggat gtattcaagt agcagaaaaa ggtgcccgca aaccaaatgg
      361 gctttgaaaa ttcttactgc cgcattttta gcagcgagtc ccgcggcgaa gagtgccgtt
      421 aataacgcct atgatgcatt gattattgaa gctcgcaagg gaaatactca gccagctttg
      481 ttatggtttg cacaaaaatc agcactcagt aataaccaaa ttgctgactg gttacagatt
      541 gccttatggg ccgggcaaga taaacaggtt attaccgttt acaaccgcta ccgttatcag
      601 caattaccat cgcgtggtta tgcagctgtc gccgtcgctt atcgtaacct gcaacaatgg
      661 caaaactcgc ttacactgtg gcaaaaggcg ctctctctgg agccgcaaaa taaggattat
      721 caacggggac aaattttaac cctggcagat gcgggtcact atgatactgc gctggttaaa
      781 cttaagcagc ttaactctgg agcaccggac aaagccaatt tactcgcaga agcctatatc
      841 tataaactgg cagggcgtca tcaggatgaa ttacgggcga tgacagagtc attacctgaa
      901 aatgcatcta cgcaacaata tcccacagaa tacgtgcagg cattacgtaa taatcaactt
      961 gctgccgcga ttgacgatgc caatttaacg ccagatattc gcgctgatat tcatgccgaa
     1021 ctggtcagac tgtcgtttat gcctacgcgc agtgaaagtg aacgttatgc cattgccgat
     1081 cgcgccctcg cccaatacgc tgcattagaa attctgtggc atgataaccc agaccgcact
     1141 gcccagtacc agcgtattca gattgatcat cttggtgcgt tattaactcg cgatcgttat
     1201 aaagacgtta tttctcacta tcagcgttta aaaaagacgg ggcaaattat tccgccctgg
     1261 gcgcaatatt gggttgcgtc ggcttatctc aaagatcatc agccgaaaaa agcacagtca
     1321 ataatgaccg agctctttta tcacaaggag atcattgccc cggatttatc cgatgaagaa
     1381 cttgcggatc tcttttacag ccacctggag agtgaaaatt atcccggcgc gctaactgtc
     1441 acccaacata ccattaatac ttcgccgcct ttccttcggc taatgggcac gcctacgagc
     1501 atcccgaatg atacctggtt acaggggcat tcgtttctct caaccgtagc aaaatatagt
     1561 aatgatcttc ctcaggctga aatgacatcc agagagcttg cttataacgc accaggaaat
     1621 cagggactgc gcattgatta cgcgagtgtg ttacaagccc gcggttggcc tcgtgcagca
     1681 gaaaatgaat taaaaaaagc agaagtgatc gagccacgta atattaatct ggaggttgaa
     1741 caagcctgga cagcattaac gttacaagaa tggcagcagg cagctgtctt aacgcacgat
     1801 gttgtcgaac gtgaaccgca agagcccggc gttgtacgat taaaacgtgc ggttgatgta
     1861 cataatcttg cagagcttcg tatcgctggc tcaacaggaa ttgatgccga aggcccggat
     1921 agtggtaaac atgatgtcga cttaaccacc atcgtttatt caccaccgct gaatgataac
     1981 tggcgcggtt ttgctggatt cggttatgcc gatggacaat ttagcgaagg aaaagggatt
     2041 gttcgcgact ggcttgcggg tgttgagtgg cggtcacgta atatctggct cgaggcggag
     2101 tacgccgaac gctttttcaa tcatgagcat aaacccggcg cgcgcctgtc tggctggtat
     2161 gattttaatg ataactggcg tattggttcg caactggaac gcctctctca ccgcgttcca
     2221 ttacgggcaa tgaaaaatgg tgttacaggc aacagtgctc aggcttatgt tcgctggtat
     2281 caaaatgagc ggcgtaagta cggtgtctcc tgggctttca ctgatttttc cgacagtaac
     2341 cagcgtcatg aagtctcaat tgagggtcag gaacgcatct ggtcttcacc atatttgatt
     2401 gtcgatttcc tacccagtct gtattacgaa caaaatacag aacacgatac cccatactac
     2461 aaccctataa aaacgttcga tattgttccg gcatttgagg caagccattt gttatggcgt
     2521 agctatgaaa atagctggga gcaaatattc agcgcaggtg ttggtgcctc ctggcaaaaa
     2581 cattatggca cagatgtcgt cacccaactc ggctacgggc aacgcattag ctggaatgac
     2641 gtgattgatg ctggcgcaac gctacgctgg gaaaaacgac cttatgacgg tgacagagaa
     2701 cacaacttat acgttgaatt cgatatgaca ttcagatttt aaggataaat atgttacgta
     2761 atggaaataa atatctcctg atgctggtga gtataattat gctcactgcg tgcattagcc
     2821 agtcaaaaac atcatttata ccgccacagg atcgcgagtc tttactcgcc gagcaaccgt
     2881 ggccgcataa tggttttgtg gcgatttcat ggcataacgt tgaagacgaa gctgccgacc
     2941 agcgttttat gtcagtgcgg acatcagcac tgcgtgaaca atttgcctgg ctgcgcgaga
     3001 acggttatca accggtcagt attgctcaaa ttcgtgaagc acatcgagga ggaaaaccgc
     3061 taccggaaaa agcagtagtg ctgacttttg atgacggcta ccagagtttt tatacccgcg
     3121 tcttcccaat tcttcaggcc ttccagtggc ctgctgtatg ggcccccgtc ggcagttggg
     3181 tcgatacgcc agcggatgaa caagtaaaat ttggcgatga gatgatcgat cgagaatatt
     3241 ttgccacgtg gcaacaagtg cgagaagttg cgcgttcccc gctcattgag gtcgcttctc
     3301 atacatggaa ttctcactac ggtattcagg ctaatgccac cggcagctta ttgcctgtat
     3361 atgtaaatcg tgcatatttt actgaccacg cccggtatga aaccgcagca gaataccggg
     3421 aaagaattcg tctggatgct gtaaaaatga cggaatacct gcgtacaaag gctaaggtaa
     3481 atccacacgt ttttgtttgg ccttatggcg aagcgaatgg catagcgata gaggaattaa
     3541 aaaaactcgg ttatgacatg ttcttcaccc ttgaatcagg tttggcaaat gcgtcgcaat
     3601 tggattccat tccgcgagta ttaatcgcca ataatccctc attaaaagag tttgcccaga
     3661 aaattattac cgtacaggaa aaatcaccac aacggataat gcatatcgat cttgattacg
     3721 tttatgacga aaaccgccag caaatggatc gcaatattga tgtgttaatt cagcgggtga
     3781 aagatatgca aatatcaacc gtgtatttgc aggcatttgc tgatcccgat ggtgatgggc
     3841 tggtcaaaga ggtctggttt ccaaatcgtt tgctaccaat gaaagcagat atttttagtc
     3901 gggttgcctg gcaattacgt acccgctcag gtgtaaacat ctatgcgtgg atgccggtat
     3961 taagctggga tttagatccc acattaacgc gagtaaaata cttaccaact ggggagaaaa
     4021 aagcacaaat tcatcctgaa caatatcgcc gtctctcgcc ttttgatgac agggtcagag
     4081 cacaagttgg catgttatat gaagatcttg ccggacatgc tgcttttgat ggcctattgt
     4141 tccacgatga tgctttgctt tcagattatg aagatgccag tgcaccggct atcgcggctt
     4201 atcagcaagc aggctttagc gggagtctga gcgaaattcg acaaaacccg gagcaattta
     4261 aacagtggac ccgctttaaa agccgtgcgt taactgactt cactttagaa cttagtgcgc
     4321 gcgtaaaagc cattcgtggt ccacatgtta aaactgcacg aaatattttt gcacttccgg
     4381 taacacaacc tgaaagtgaa gcctggtttg cacagaatta tgctgatttc ctaaaaagct
     4441 atgactggac cgctattatg gctatgcctt atatggaagg tgtcgcagaa aaatcggctg
     4501 atcaatggtt aatacaactg accaatcaga ttaaaagcat ccctcaggct aaagacaaat
     4561 ctattttaga attacaggca caaaactggc agaaaaatgg tcagcatcag gctatttctt
     4621 cgcaacaact cgctcactgg atgagcctat tacaactgaa tggagtgaaa aactatggtt
     4681 attatcccga caattttctg cataacaaac ctgaaataga cgttattcgt cctgagtttt
     4741 caacagcctg gtatccgaaa aatgattaat cgcatcgtat cgttttttat attatgtctg
     4801 gtgttatgca tccccctatg cgtagcgtac tttcactctg gtgaactgat gatgaggttc
     4861 gttttcttct ggccgttttt tatgtccatt atgtggattg ttggcggcgt ctatttctgg
     4921 gtctatcgtg aacgccactg gccgtgggga gaaaacgcac cagcccccca gttgaaagat
     4981 aacccgtcta tctccattat cattccctgt tttaatgagg agaaaaacgt tgaggaaacc
     5041 atacacaccg ctttagcaca gcgttatgag aacattgaag ttattgccgt aaatgacggt
     5101 tcaacagata aaacccgtgc catcctggat cgcatggctg cacaaattcc ccatttgcgg
     5161 gtcattcatc tggcgcaaaa ccaggggaaa gccattgcgc ttaaaaccgg agctgccgcg
     5221 gcgaaaagtg aatatctggt gtgcattgat ggcgacgcgt tattagaccg cgatgcggcg
     5281 gcatatattg tggaaccgat gttgtacaac ccgcgtgtgg gtgccgtaac cggtaatcct
     5341 cgtattcgca cacgttctac cctggtgggt aaaattcagg ttggcgagta ttcctcaatt
     5401 attggtttga tcaagcgaac ccagcgtatc tatggaaacg tatttaccgt ttccggtgtt
     5461 attgccgcat ttcgtcgcag cgccctggca gaagtgggtt actggagtga cgatatgatc
     5521 accgaagata ttgatattag ctggaagctg cagttgaatc agtggacgat tttttacgag
     5581 cctcgggcgc tgtgctggat attaatgcct gaaacgttaa aagggctgtg gaaacagcgc
     5641 ctgcgctggg ctcagggcgg tgcagaagta ttcctcaaaa atatgacaag gctatggcgc
     5701 aaagaaaact ttcgaatgtg gccactgttt tttgaatact gcctgacgac aatatgggcc
     5761 ttcacctgcc tggtcggttt cattatttac gcagtccaac ttgccggtgt accgttaaat
     5821 attgaattga cacatatcgc tgcgacacat actgccggaa tattattgtg tacgttatgt
     5881 ttactgcaat ttattgtcag cctgatgatt gagaatcgct atgagcataa tctgacttca
     5941 tcgcttttct ggattatttg gttcccggtt attttctgga tgctgagcct ggcaacgaca
     6001 ttggtatcat ttacacgagt catgttgatg cctaaaaagc aacgcgcccg ttgggtaagt
     6061 cccgatcgcg ggattctgag aggttaatat gaacaattta attattacga cccgacaatc
     6121 gccagtacgt ttactggttg attatgttgc cacaaccatc ttgtggacat tatttgcttt
     6181 gttcatattc ttattcgcca tggatctgct gacgggttat tactggcaaa gcgaggcccg
     6241 aagccgactt cagttctatt ttttgctggc agtggcgaat gccgtcgtgt taattgtctg
     6301 ggcgctgtac aataagctgc gttttcaaaa acagcagcat catgcagcct accaatatac
     6361 gccgcaagaa tatgcagaga gcttagcaat acctgatgag ctctatcagc aactacaaaa
     6421 aagccacagg atgagcgtac acttcaccag ccaggggcaa ataaaaatgg tggtttcaga
     6481 aaaagcgcta gtccgggcat aaacacccaa aacaaagccc ggttcgcccg ggctctgcac
     6541 cgataataca cttaactgta tgcatgcagc gtacgttggc aaagtgccga acgtacgcaa
     6601 tcctctttac cgaaccggac gatcccgacc atttcatctt cttcgaaacg ttccagcgcg
     6661 tcacttaatc cggagcacac gccgcgaggc aaatcgcatt gcgtgatatc accgttgacg
     6721 ataaccgtca cgttctcccc gaggcgggtt aaaaacattt tcatttgcgc ggcagtcaca
     6781 ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg tacgtccacg catataggcg
     6841 aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc agtactgcat aaaggaagcc
     6901 cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat agggggcaaa cttttctgcg
     6961 atatctccag gtaagaagcc aagatcttca tcggcttgca gaactggacg ggtgacgata
     7021 atcctgtcga catccttatg tatcagggcc tctgccgctt ttgctgcact gatccaggtt
     7081 tttccgcacc cggcttcgcc cgtagcgaat atcagctgct tactctcaat agccttcaga
     7141 tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag tatcgcgact gtcgcgggcc
     7201 ataccaatgg cttctacgcc gctcatctgc acaagcgagg tgaccgattc ttcttcacgc
     7261 tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg cctcgcgacg agctttgatc
     7321 actgcttttt gtcttcccat ggagagcacc ttgagttgtt tgtattcatc acacgcgccg
     7381 ttggcagcgc gattatgcgc acgaacatca gagggttggc ttccttgtaa gccatagttt
     7441 gcttttggat aaaatgccga aaaacggcta cgcgcaccgt ttacggcgtc ggtaacacat
     7501 gaaaagaaag gatgaggttg aaaatgcaaa gtgacgagat gactaccgga ggagaaaact
     7561 ccgcgagtgg tggcgcgttg attatctaaa acatgtccag tacaggacgt taccatccgc
     7621 gatctccata gtgactgact atcactgccg ggaacttccg ctgctactta ataagtacaa
     7681 cagatctcgc attcattgca acaatatatt cacttatatt taactataaa acaccatttt
     7741 aatgacatta gtttctactg gaaagatgac agagtgatga cagagatgaa aaaagctgtg
     7801 tgctttcagc aggattcaca tcaggcatca gtgcacgatc gccggatgcg gcgtgaacgc
     7861 ctgatccagc ctaccaaacc cgcaccgatt gtgagcatca taagcatcgc aagcgccgca
     7921 tcaggcatct gcgcacaaat gccttatccg cctaaaaaca ttaaacccgc aataatgcgc
     7981 ttccgagata atcgttcgcg tccttcactc ccggcagcgc aaaaaaataa ccgccgccga
     8041 taggtttaac gtattcctcc agcgcttcgc cattgagcct tttttgtact gtcaggaagc
     8101 ctttttccag atcgtgttgg tagcagacaa acagcaaccc catatccagt tgtccggagt
     8161 tggtgacgcc cagtgaataa ctgtagccac gacgcagcat caggctggac tcactctccg
     8221 ccgtgcgggg attcgccagc cggatatggc tgtccaacgc aatcaccttc ccttccgggt
     8281 cgctggcgta atcaggcaca tcatgctcat gctgcattcc cagcggcgca ccggtttgct
     8341 tatcacggcc aaaaatcgtc tgctgttctt tcagcggcgt tctgtcccaa aattccactc
     8401 gaaactgaat caagcgtact gcctgatagc tgccaccgat tgtccacgca ggctcctgct
     8461 gatctgccgt tacccacacc actttttgca tcaacttatc attctggcta tcgggattgg
     8521 cagtgccgtc tttgaaaccc agcaaattaa tcggcgtctc tttgccttta ctacgcgccg
     8581 cgtgatctga aataaaccct tcccgcttcc agcgcacact gagcaaatcc ggcgtgtgtt
     8641 tgatgatatc gcgcagcgca tggatcaccg tgtcctgggt gttggcgcaa atctgtagca
     8701 acacatcacc atgacataac gccgcatcca gcgagtcgtt ggggaaacgc gtcatcttct
     8761 gcagcttttt tggcatctgt ggcgcaaggc caaagcgctc atcaaacaat gagtgaccca
     8821 ccgataacgt gatggtgaga ttatcgggcg caatgtagcc gccaagaatg ccggaatcga
     8881 gtggtggcag gcgcggattt ggcgtttctg gtgccgctcc gccctgagtc agaaaagcaa
     8941 aacgctgagt caacaagcga aaaaaccgct caagatcggc tttatcgctg gcaagcacat
     9001 caaacgccac cagcatcatt gcggcctgtt gtggcgtcag gatccctgcc tgatgctcac
     9061 cataaaacgg ctgtttctca ttgcgtgcat ccggtgaaag cgtgcccggt gcactttgcg
     9121 ttttttgtgc atgagcgacc ggacaacttc ccgccagcgc cagcgcacca attcctttca
     9181 gtaaacgtcg gcgtgacagt tcattcacgc cgttttcatc ttcatactgc ataacgctta
     9241 atccagtccc agcacaccac gaagttgcgc cagatcttcc gccagcgcag taatcggccc
     9301 tttcagcgcg ttccggtcgg cctcggtcaa tttgtcgtag gtttcaaaac cgtctttagt
     9361 acggtatttc gccagaatgg tatcgacctt tttaaagttg gcatcgactt ttgccagcag
     9421 ttccggattg gctttttgca gttgtggacg cagcagatcg acaattttct gcgagccttc
     9481 aacgttagcc tggaaatccc acagatcggt gtggctgtag cgatcttctt caccgctgat
     9541 tttgctggct gccacttcct caatcagtcc ggctgcgccg ccgaccactt ttgaaggtgg
     9601 gaaagccagt tcactgatgc gtttttgcaa atcgaccaca tcggtataaa gctggtcggc
     9661 atacttatcc atccctttgg tggtgttgtc gccaaacaat gctttttcca gacggtggaa
     9721 accggtgaat tttggatcag cagctttttg ctcgtaatca tcttcacggg cgtcaatgct
     9781 gccatccaga tcggagaaca gttcagcaat cggttcaatg cgctcatagt gctggcgcgt
     9841 cggcgcatac agtgctttcg ctttttcgat atcgcctgct ttaatcgcgt cggtaaaggc
     9901 tttggtgtcg gtcaccagct gtgtggtttc cgccatgaca tacgctttat atgcagtaat
     9961 tgcaccacca agacttaaca gcgcatcact ttgcgccgca tccgccgttg cctcaccttt
    10021 gacaatcaac ttccctttcg ggttagtcag cagaccgcag gtcatatcat attcgccagg
    10081 ctgtaaattc gccgtcattt tctggctaaa accaggggcg atattttccc gctcttccac
    10141 caccatcacg cctttgagga tctcccactc cagcgccttc tggctatggt tctgaataat
    10201 gaactgtgtt ttcccggcgt taaccgtaat ggtcatcggt tcgcactgtt tatccgtcac
    10261 ggtcactttg acttgcggaa tatcagcggc gttagccata aaagcagaag agaacagcgt
    10321 agccacgctc aactgcaatg cgttacggcg gaagttaatg gtcatgacga gactatccct
    10381 ttaaagtatg ttgtaactaa gcaagagttt gcgtcgtatt tgctacgcgg agcgagacgc
    10441 tgtcgcccct gcgcgtggtg gcagagcaaa tgccaccagc gccaggatga gataaataaa
    10501 ccagacggcg acttcgctga cgctcggcgc ttcctgataa ccaaaaatcc cttccatcaa
    10561 cgtgccaaac agcgagtgag ttgagagcac ctcgctcata tcaaaggcga tttcctgaaa
    10621 gtggttccac aatccggctt catgaaatgc gcgaatggca ccagctgcca gccctgcggc
    10681 gacgaagaga ataaacaggc tggtccattt aaaaaatgca ccaagattga ggcgaatacc
    10741 gccccagtag agcaggaagc cgagcaccac ggcagtagca agaccgagca ttgcacccag
    10801 cggcggccag atcccgacat cttgttgaaa tgccgccagc aggaaaaaga ccgactccag
    10861 gccttccctt gcaacggcaa aaaagaccat catcaccagc gcccagccat gatgatttcc
    10921 acgctgcaat gcgctatcga ctgcctgttc cagttgcact ttgacgttgc gcgacacttt
    10981 gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca cgataccttc
    11041 aaacagttcc tgctcttttt gcggaaattc gccggtggtt tcgttaatga agatacccaa
    11101 gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacaccaa tccatcggcc
    11161 tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg cttcaagtcc
    11221 ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac gtttttctga
    11281 gtcaatctat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac gctaaagaaa
    11341 aatacaagcg gaatgtttat aaaatgatgt tggaatgtaa aaatgaggag ttcaaatccg
    11401 gagaagaaag cgattatttt aaggtattta gctgacgttt cgcccggcag atgaagcttg
    11461 tacgaatgtt agtgcccaca aactcgctgc agcgtgcccc gaagggttga attttcagtt
    11521 agtcgtatca caaaagaacg ttgttttttg cttattaata gatagtgata aggttgtgaa
    11581 tgaaattata cctcatctcc tcatcgaatt ttttgcccac aacctaaaca ggagaaaacg
    11641 tgtatttaag t
//
LOCUS       DAPGRN010000132        11613 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPGRN010000132 DAPGRN010000000
VERSION     DAPGRN010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.134"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            257..1027
                     /locus_tag="RHW33_003972"
     CDS             257..1027
                     /locus_tag="RHW33_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV1647427.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKSHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1018..1491
                     /locus_tag="RHW33_003973"
     CDS             1018..1491
                     /locus_tag="RHW33_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV1647428.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1598..2137
                     /gene="dnaT"
                     /locus_tag="RHW33_003974"
     CDS             1598..2137
                     /gene="dnaT"
                     /locus_tag="RHW33_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV1647429.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2140..2877
                     /gene="dnaC"
                     /locus_tag="RHW33_003975"
     CDS             2140..2877
                     /gene="dnaC"
                     /locus_tag="RHW33_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV1647430.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2926..3420
                     /locus_tag="RHW33_003976"
     CDS             2926..3420
                     /locus_tag="RHW33_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV1647431.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3674..5965
                     /gene="opgB"
                     /locus_tag="RHW33_003977"
     CDS             3674..5965
                     /gene="opgB"
                     /locus_tag="RHW33_003977"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV1647432.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6102..7124)
                     /gene="lgoD"
                     /locus_tag="RHW33_003978"
     CDS             complement(6102..7124)
                     /gene="lgoD"
                     /locus_tag="RHW33_003978"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV1647433.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7263..8177
                     /locus_tag="RHW33_003979"
     CDS             7263..8177
                     /locus_tag="RHW33_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV1647434.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8392..9753
                     /locus_tag="RHW33_003980"
     CDS             8392..9753
                     /locus_tag="RHW33_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647435.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9802..11466)
                     /gene="tsr"
                     /locus_tag="RHW33_003981"
     CDS             complement(9802..11466)
                     /gene="tsr"
                     /locus_tag="RHW33_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV1647436.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
BASE COUNT         2814 a         3067 c         2823 g         2909 t
ORIGIN      
        1 ttaaaaaatg tgcccggtta tctattatcc tgattcgtaa aggctaaaca ataaattcat
       61 tatgatgata atattcatgt gatagtttgt ttgcggcgag agataattcg ctttttatca
      121 ccaagcgtct cccgataacc aggattaatc agcgcaattt tgatcttaac ctaccgcaaa
      181 acaaccgtgc ttcatgatac aaatggattc tcattatctg aacaatacgc atcacgtcta
      241 tgacaaaggg cgagttatgc aaactgagca acagcgagcc gtaacgcggc tttgtatcca
      301 gtgtggatta tttcttttgc aacatggtgc ggaaagcgcg ttggttgatg agctttcctc
      361 acgactgggt cgggcgctgg gaatggacag cgtcgaaagt tctatctctt cgaacgccat
      421 agtgctgaca actattaaag atgggcaatg cctgacatcg acacgtaaaa gtcacgatcg
      481 cggcattaat atgcatgtgg tgactgaagt ccagcacatt gtgattcttg cggagcatca
      541 tctgctggat tacaaaggcg tagagaaacg atttagccaa attcagccat tacgttaccc
      601 aagatggctg gtagccttaa tggttggcct ttcttgcgcc tgtttctgta aactcaataa
      661 cggtggctgg gatggtgccg tcatcacctt ctttgccagt acgaccgcga tgtatatccg
      721 ccagctgctg gcacaacgtc atcttcatcc acagatcaac ttttgcctta ccgctttcgc
      781 cgccaccacc atttccggat tgcttttgca actccccact ttcagcaata cccccaccat
      841 tgcgatggcc gccagcgttc tgctgctggt gccgggcttt ccgttgatta atgccgtcgc
      901 cgatatgttt aaaggccaca tcaataccgg actggcacgc tgggcgatcg ccagtctgct
      961 gacactggct acctgcgtcg gcgtggtgat ggcactgacg atttgggggc tacgcggatg
     1021 ggtgtgatcg aatttctgtt tgcattggcg caggatatga tcctcgccgc cattcctgcg
     1081 gtcggctttg cgatggtgtt caacgttccc gtgcgggcgt tacgctggtg tgcgctgctt
     1141 ggctcgatag gtcatggttc ccgaatgatc ttgatgacca gcgggttgaa tattgagtgg
     1201 tcaaccttta tggcttctat gctggtcggc accattggta ttcaatggtc gcgctggtat
     1261 ctggcacatc cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc gggcatatcg
     1321 gcttataccg caatgatttc ggcggtaaaa atcagccagt taggttacag cgaaccgttg
     1381 atgattaccc tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc gttatccatc
     1441 ggtctttcca ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata aaattgcctc
     1501 gcttaagcac acggatgaga gacagcctcc tctcctccgt gtgttactat aaaagttatc
     1561 tcccttctcg ttcatcgttc catatttgag aaacagtatg tcttccagag ttttgacccc
     1621 ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa accgttctgg caaaagctga
     1681 aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc
     1741 acgcctggct gaactgctgg cgctggaaga aaagctggcg cgtccgggaa gcgatgtcgc
     1801 tctggacgat caactctatc aggaaccgca agccgctccc gttgctgtac ccatggggaa
     1861 attcgccatg tatccggact ggcaacccga tgccgatttt atccgcctgg cggcgctatg
     1921 gggcgtggcg ctaagagagc cggtgaccac cgaagaactg gcctcattca ttgcctactg
     1981 gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct
     2041 gcaaatcggt cgtgccagca acggcggact gccgaaacga gatgtgaata cggtcagtga
     2101 acctgacagc caaattccac caggattcag agggtaacga tgaaaaacgt tggcgacctg
     2161 atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa aacgggtgaa
     2221 gagctactgg cctggcagaa agaacaaggg gcgatccgct ccgccgctct cgaacgtgaa
     2281 aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc actgcatcag
     2341 aactgttcct ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa
     2401 gcgcgccagt atgtcgaaga gtttgacggc aacatcgcca gcttcatctt ttctggtaag
     2461 ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca acgagctgct gctacgcggt
     2521 aaatccgtat tgatcatcac cgtggccgat attatgtcgg cgatgaaaga taccttcagg
     2581 aatagcggta ccagcgaaga acaactgctt aacgatctga gcaacgttga tctgctggtg
     2641 atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat caaccagatc
     2701 gtcgatcgcc gctcttcttc caaacgccca accgggatgc tgaccaacag taatatggaa
     2761 gagatgacca agttgctggg cgaacgcgtg atggaccgta tgcgcctggg taacagtttg
     2821 tgggtgatct tcaactggga tagctaccgt agccgggtaa caggtaaaga gtattaatcc
     2881 ccgtttctgg cggtatactg cgacacaatt ccaccttgag taatgatgat gaaaactgtt
     2941 aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg ggtgcatgct
     3001 gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac ttggcaacca aaacagcacg
     3061 acacaggaag gcggttggtc gctcgcgtca ttaactaact tgcttagcag cggaaaccag
     3121 gccttaagcg cagataacat gaacaatgcc gcaggcattc tgcaatactg cgcgaagcaa
     3181 aagctggcgt cggtaaccga tgccgaaaac atcaagaatc aggtgctgga aaagctgggc
     3241 ctgaacagtg aagagcaaaa agaagacacc aactatctgg acggcattca gggtttgctg
     3301 aaaaccaaag atggtcagca actcaatctg gataacatcg gaacgactcc gctggcagaa
     3361 aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt catttcctga
     3421 accgcgaaac gccgcgtttt agctgttacc cggctgacgc ggctctctcc tgcccttttc
     3481 cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca aaaaagtcta
     3541 tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc agcaatatgg
     3601 cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag
     3661 tgaggatcgc ccgttgtcag aactactctc tttcgccctt tttctcgcct ctgtgctgat
     3721 ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacgttaa cggtgctggg
     3781 gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg gcgatggtat
     3841 taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata
     3901 catcctgccg ggtatcggca ttgtgctggg gctgacggcg gtgttcggtg cgctgggctg
     3961 gatcctgcgc cgtcgtcgtc atcatccgca ccattttggt tacagcctgc tggcgctctt
     4021 actggcgctg ggttcggtgg acgccagccc ggcatttcgt cagataacgg aactggtgaa
     4081 atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc cgtcgaaaac
     4141 tatcccagac ccgaaactta acctggttta tatctacggc gaaagtctcg agcggaccta
     4201 ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga aaaatgaagg
     4261 cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg cgggcatggt
     4321 ggcctctcag tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct ccgcttctgt
     4381 ttccagcttc ttcccgcaga acatctgtct gggcgatatc ctgaaaaact cgggttatca
     4441 gaactatttc gtgcagggcg caaatctgcg ttttgccggt aaagatgtgt tcctgaaatc
     4501 gcacggcttc gaccacttat acggctcaga agagctgaaa agcgtggtag ccgacccgca
     4561 ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt ggaaaaagtt
     4621 tgaagagctt tcccgctcag gtcagcgatt ctcactgttt accctgacag tcgataccca
     4681 tcacccggat ggttttatct cgcgcacctg taaccgcaaa aaatatgatt ttgacggtaa
     4741 gccgaatcag tcattcagcg cggtaagttg cagccaggag aatatcgcga cgtttatcaa
     4801 caaaatcaaa gcgtcaccgt ggtttaaaga tactgtcatc gtcgtctctt ctgaccattt
     4861 agcgatgaac aacacggcgt ggaaatacct caataagcag gatcgcaata acctgttttt
     4921 tgttattcgt ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta acacgatgga
     4981 taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac ttggtcgtag
     5041 cagtttatcc gggcagtcga tgtcggaaat tttcctcaat atcaaagaga aaacattggc
     5101 gtggaagcca gatatcatcc gcctgtggaa attccctaaa gagatgaaag agttcaccat
     5161 cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc cgctgctgtt
     5221 gcgggtttca gacaaacgcg tggaaccgct gccggaaagc gaatactcag caccgctgcg
     5281 tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgacc gttgctacaa
     5341 gatggcacaa ctctgggctc cggaactggc actttccacc gactggtgtg tctcgcaggg
     5401 gcagcttggc ggtcagcaaa ttgttcagca tgttgacaaa acaacatgga agagcaagac
     5461 ggcatttaaa gatacggtca tcgacatggc gcgttacaaa ggcaatgtcg atacgctgaa
     5521 gattgttgat aacgatattc gctacaaagc cgacagtttc atctttaacg tcgccggtgc
     5581 gccagaagag gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg gccgctggtc
     5641 caacgcgcaa ctgggcgatg aagtaaaaat cgagtacaag catccgctgc cgaagaaatt
     5701 tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta ttccggtacg
     5761 cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa gtgaccacca ccacactgca
     5821 tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac ctgtctcaac
     5881 caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca tggtggaaat
     5941 taaagtggta gaacgtgaag ggtaattcct gactgatgtg taggtcgcat aaggtgcgac
     6001 ctacacacct tctccggctt acaacacatt attaagaaac aaaaaaacag cgcccaacac
     6061 ttccccggac gctgcatggc aactttaata atctcgcctt atcagaaagt aattacaccc
     6121 ttaatcaact cacgattgtt aatcacatcg cgctcgtagg tttctgccag cgtggcgaac
     6181 gggtagcgat gggttaacat catgtcggca gtgatttttc cttccgccat cagtcgaccg
     6241 actttagcaa aatcttccgg cgtggcgttg cggctgccca tcatcgtcgt ttctttttta
     6301 tggaattccg gatcggagaa ctgcaactca cctttaaaca ggccgacaaa taccaccgtg
     6361 ccgccgtgac gaatcagatt cacggtgtta ttcatcgcat gttgattacc tgtcgcgtcg
     6421 atcactttct gcgccagcga accaccaaac tgcgcccgca gctgcgcgtc aaaatcttcg
     6481 gctgacgggt ccagcacggg caactccaga cgcgttgcca catgttcacg gcgcgccgga
     6541 ctggtatccg ccaccaccac ctgtgcgcca tcggctttag cgattgccgc cgcgcccaga
     6601 ccgattggcc ccgccccgac caccagcacc tgctcgccgg gagcaatggc tgcgcgacgc
     6661 accgcatgag cgctaatagc gaaaggttca atcaatgcgg ccgcctgcgg gtcaataccg
     6721 tctgcgggca aaatgttcgc caccggcacg ctcaaatact cactaaaacc gccatcctga
     6781 tgcacgccaa tgactgaaat tttttcacag caattggtac gcccgctttt acacgccggg
     6841 cattgctgac aggcaacata agggatcaca gcaacttgct gaccattttt aagatcagca
     6901 atatttttac ccagcccaac aatctcccca catatttcat ggcctaaaac ccgtggataa
     6961 ctaaaaaatg gttgatttcc accccaggca tgaatatcgg taccgcaaat cccgacagac
     7021 ttaattttta ttaatgcttc attgtcaccc ggaataggta tctcgcgttg tttccagact
     7081 aattctttcg gctgctggca aattaaaaca ttcatcgtag acatgattcg gctctccatt
     7141 ttttgttgcc ttagatatca atgaaaatga aaaatggcag cggaacggaa aatctgtttt
     7201 gtgaaaacac acgcataaaa atgttttaaa tcgggtttta atggactcac aaggaggaaa
     7261 atatgagtcg ttcacaaaat ttacgccaca atgtgattaa ccaggtcatt gatgatatgg
     7321 cgcggggtca tatcccttcc ccacttccat cgcaaagtgc gctggcggaa atgtacaaca
     7381 tcagccgcac tacagtgcgt catattctca gccacttacg cgaatgcggc gtcttaacgc
     7441 aggttggaaa cgactatgtt attgcgcgta aacctgacca tgatgacggt ttcgcctgta
     7501 ctaccgcttc aatgagtgaa caaaacaaag tgtttgagca ggcttttttc acgatgataa
     7561 accagcgtca gttacgccct ggggaaactt tttctgaact gcaactggct cgggccgcgg
     7621 gtgtcagccc ggtggttgta agagaatatc ttttaaaatt cggccgttat aatcttattc
     7681 aaagcgaaaa gcgcggccag tggagcatga agcaattcga tcaatcctac gccgagcagc
     7741 tgtttgagct acgcgaaatg ctcgaaacac attcacttca gcactttctc aacctgcctg
     7801 atcatgatcc ccgctggctg caggcgaaaa ccatgctcga acgtcatcgg ctactgcgtg
     7861 acaacatcgg caatagcttt cgcatgttct cgcaacttga cagagatttt cactccttac
     7921 tgctttcagc tgccgacaat atcttttttg atcaatcgct tgagatcatc tcagtcatct
     7981 ttcattttca ctatcaatgg gacgaaagcg atcttaaaca gcgcaatatc attgccgtcg
     8041 atgagcatat gaccattctc agcgctctga tttgccgcag tgaccttgat gccacccttg
     8101 cgctccgtaa ccatttgaat tcagcgaaac aatcaatgat tcgatccatt aatgagaaca
     8161 cgcgttatgc tcattaagta cggattaaaa acagcaaact attagcgggt gcacggtttt
     8221 atcaaaagat aaataccggg aaattcccgc ttacctatgc tcacaatcag tcgactaaaa
     8281 atgtttttgt gagcggtagt aaagtcctaa aactttaacc tgccgttaat gtcaaccttg
     8341 cgtaatgcac aattacgtaa acaattaaaa ttataaatac aggagtccgg cgtggaaaaa
     8401 gaaaatatca ccctcgatcc gcgttcttca tttactccat cttcgtcggc agatattccc
     8461 gtgccaccag atggattagt tcaacgcagt acccgaatta aacgcattca aaccaccgcc
     8521 atgttgttat tattttttgc ggcggtaatc aattatctcg accgcagttc gctgtcggta
     8581 gcaaatttga cgattcgtga agaattgggc ttaagtgcca ccgaaatcgg cgctttgctc
     8641 tccgtgtttt cactcgctta cggcattgcg caacttcctt gcggcccact attggatcgt
     8701 aaaggcccac gcctgatgct gggactgggg atgttcttct ggtcactgtt ccaggccatg
     8761 tctggcatgg tgcacagctt tacgcagttc gtgttggtgc gtatcggtat ggggattggt
     8821 gaagcgccga tgaacccatg cggtgtaaaa gtcattaacg actggttcaa catcaaagag
     8881 cgcggacgcc cgatgggctt cttcaacgca gcttctacca ttggcgttgc cgtaagccca
     8941 ccgattctgg cggcgatgat gctggtgatg ggctggcgcg ggatgtttat taccattggt
     9001 gtactgggga tttttctcgc catcggctgg tatatgctct atcgcaaccg cgagcacgta
     9061 gaactgactg ccgttgaaca agcttatctc aatgcaggta gcgtcaatgc ccgccgagat
     9121 ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc gcacaatgtg gggaatgatg
     9181 ctcggattca gtggcatcaa ctacactgcg tggctgtatc tggcctggct tcctggttac
     9241 ctgcaaacag cctataacct ggatttaaaa agcacagggt tgatggcggc catccctttc
     9301 ctgtttgggg ctgccgggat gctggtcaac ggttacgtta ctgactggct ggtcaaaggg
     9361 ggaatggctc cgattaaaag ccgtaagatc tgcattattg ccgggatgtt ctgttctgcc
     9421 gcctttacgc tgatagtacc acaagcgaca acatccatga tggcggttct gctgattggc
     9481 atggcactgt tctgtattca ctttgccgga acatcctgct ggggcttgat ccacgtcgca
     9541 gttgcttctc gcatgactgc gtcggtgggc agtatccaga actttgccag cttcatctgc
     9601 gcctctttcg cgccgatcat tactggtttt attgttgata caacccactc attccgtctg
     9661 gcactaatca tttgcggttg cgtcaccgca gcgggagcac tggcgtacat cttcctggtt
     9721 cgtcagccga tcaacgaccc gcgtaaagat taaactccct atgcccgata acattttgct
     9781 tatcgggcat ttttatggcg attaaaacgt ttcccagttc tccccgctat ctgccactgc
     9841 cattttacgc ggcgtagctg gcgtcacggt ttttaccaca gccgatgttt cacgctgctg
     9901 ttgctgctgt tgctgaatcc ggaacactgc cacagcttcg gtcaggcgac tggcctgctc
     9961 ttccaacgcg gcggcggcag cggcagactc ttccaccagc gcagcgttct gttgagttac
    10021 ccggtccatc tcagcaaccg ctaagccaac ctgatcgata ccacggctct gctcatcaga
    10081 agcagaagct atttcgccca taatgtccgt cacgcgggtc acggcgctga caatctccgc
    10141 cattgtttcc ccggcgcttt cgaccagcgt agagccaaca tccactttcc ccaccgagtc
    10201 ttcaatcagg cttttaattt cacgagccgc ctgggcgcta cgctgggcca gattacgcac
    10261 ttctcccgca accaccgcaa aaccacgtcc ttgctcaccc gcacgcgcag cctcaaccgc
    10321 cgcgttcaaa gccagaatat tggtctggaa ggcaatgccg tcaattacgc tgataatatc
    10381 ggcgattttc tgcgaactag tggagatatc gcgcatagtc tgcaccacgt tatccaccac
    10441 tttgccgccg cgttgcgccg tttcagaagc acttaacgcc aggtggctgg cctggcgcgc
    10501 attctcggcg ttctgtttca ccgttgcggt cagttgctcc atgctggctg ccgtctcttc
    10561 cagcgaagcg gcctgttgct cggtgcgcga agagagatcg ttattaccgg tagcgatttc
    10621 gctggcaccg ctatagatgg cattggcccc gttgcgcaca tcaccgacgg tacgcatcag
    10681 ttctccctgc atatggcgca aactctctgc cagttgcccc atctcattag agccatccac
    10741 ctcaatcggt ttcaccagat cgccgcctgc aatatgacga atgctgtcaa tcaagcgatt
    10801 cattggcgct accagcgagg ctttaatacc gaaccagacg gcgaagatga ccgccagtac
    10861 gacgatcatc acgcccacca gaatccacat cgcctggctg taggaggcat tgttatcgct
    10921 gacggcgata tcatagagcc gatcgttttg ctccatgtaa gccacatact gcttctcgaa
    10981 accgtcctga tatccctggg tcggctgatc aaagaactcg ttgattttgc ctgcacctaa
    11041 cagttggatc agctccgcca gcgcattgtg ataaatatcg taattacgtt tgatctctgc
    11101 cgctgcggcg gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg cccagttttt
    11161 ttccgcctgt ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg ttgaaccgct
    11221 accaatatta ttctgatcca tcatgtagcg gatacccgcg cggttgaggg tgttacgcgt
    11281 ctgcaacaac gcgacccagc tgccattcag cgtggattgc tgctgacgaa tggtttgtaa
    11341 aacagtgaaa ttttctttgt cattctttaa ggcattaaag aacagaccgc ctgatgtcag
    11401 ttgtaaaagg ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct tgatacgttt
    11461 taacatggtt tctctttcct gtggaccgat gcaagatttt cggcctgaaa tgagaaaact
    11521 ttataggtag cgacttgaaa ctggttaatt ttacctcact tttttaattt ttgttcaaaa
    11581 atacaataac cacttattga tacattttct ttt
//
LOCUS       DAPGRN010000133        11383 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPGRN010000133 DAPGRN010000000
VERSION     DAPGRN010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.135"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1831)
                     /gene="ccmF"
                     /locus_tag="RHW33_003982"
     CDS             complement(<1..1831)
                     /gene="ccmF"
                     /locus_tag="RHW33_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV1647437.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIFAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAVLRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFV"
     gene            complement(1828..2307)
                     /gene="ccmE"
                     /locus_tag="RHW33_003983"
     CDS             complement(1828..2307)
                     /gene="ccmE"
                     /locus_tag="RHW33_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV1647438.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(2304..2513)
                     /gene="ccmD"
                     /locus_tag="RHW33_003984"
     CDS             complement(2304..2513)
                     /gene="ccmD"
                     /locus_tag="RHW33_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV1647439.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(2510..3247)
                     /gene="ccmC"
                     /locus_tag="RHW33_003985"
     CDS             complement(2510..3247)
                     /gene="ccmC"
                     /locus_tag="RHW33_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV1647440.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(3289..3951)
                     /gene="ccmB"
                     /locus_tag="RHW33_003986"
     CDS             complement(3289..3951)
                     /gene="ccmB"
                     /locus_tag="RHW33_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV1647441.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(3948..4565)
                     /gene="ccmA"
                     /locus_tag="RHW33_003987"
     CDS             complement(3948..4565)
                     /gene="ccmA"
                     /locus_tag="RHW33_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV1647442.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(4584..5186)
                     /gene="napC"
                     /locus_tag="RHW33_003988"
     CDS             complement(4584..5186)
                     /gene="napC"
                     /locus_tag="RHW33_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV1647443.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(5196..5645)
                     /gene="napB"
                     /locus_tag="RHW33_003989"
     CDS             complement(5196..5645)
                     /gene="napB"
                     /locus_tag="RHW33_003989"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV1647444.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(5642..6505)
                     /gene="napH"
                     /locus_tag="RHW33_003990"
     CDS             complement(5642..6505)
                     /gene="napH"
                     /locus_tag="RHW33_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV1647445.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(6492..7187)
                     /gene="napG"
                     /locus_tag="RHW33_003991"
     CDS             complement(6492..7187)
                     /gene="napG"
                     /locus_tag="RHW33_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV1647446.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(7194..9680)
                     /gene="napA"
                     /locus_tag="RHW33_003992"
     CDS             complement(7194..9680)
                     /gene="napA"
                     /locus_tag="RHW33_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HDV1647447.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQDVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(9677..9940)
                     /gene="napD"
                     /locus_tag="RHW33_003993"
     CDS             complement(9677..9940)
                     /gene="napD"
                     /locus_tag="RHW33_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV1647448.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(9930..10424)
                     /gene="napF"
                     /locus_tag="RHW33_003994"
     CDS             complement(9930..10424)
                     /gene="napF"
                     /locus_tag="RHW33_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV1647449.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            10831..11319
                     /gene="eco"
                     /locus_tag="RHW33_003995"
     CDS             10831..11319
                     /gene="eco"
                     /locus_tag="RHW33_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV1647450.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2769 a         3354 c         2909 g         2351 t
ORIGIN      
        1 gaacaaaagg tttgtagtag agacgaacag cccaggcacc gttttccagt tcttcaccga
       61 gcgcggcgta caggtcacgg gtgatgccgc cgtcaatcgc cgcttcggtc atcatcgacc
      121 cggcagtgtt gtaataacgt ttttccgcat acagcaccgt ttccggcttg ccatctcgcg
      181 ttacgccgat agtcgccaca ccgccacgcc agttcgggcc agtcacctct ttgacatcac
      241 ggaaagtgaa gcgatattca tgaatatcga cgctatcgcc ggacttcatg cgcacatcac
      301 gctcaacgct gtagttctgg ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt
      361 gagccgccac catcccccaa taactgaagg tggttttcgt gccgcgtgaa atacgcagca
      421 cagcttccgc aattgccagc accgcaatcc agcaggccat tgccaggccg agcaccgtca
      481 tcgccaccac tttgctttcg aacagccacg gcaacagcag agacagcacc agcgtagaga
      541 taaaggcgaa aatcaataaa ttgcggatct tacgtgggcg atcgcgcccc cagcgcacca
      601 gaggaccgac acccagcagt agcgcaaacg gcaccatcag ccaggtaaac atggtgttga
      661 agaacggttc gccaatcgaa atactgccca gtcccagttg cttgtgcacc agcggcagca
      721 gcgtccccag caacaccacc agcatcgcgg cgaccagcaa aacgttgttc gctaacagca
      781 aagattcccg cgaccacagc gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg
      841 caaacagcag cagcgaaccg ccaatcacca gcaccataaa ggcgaggata aacataccgc
      901 gcgccggatc agacgcaaac gcgtgtaccg ataccagcac gccggaacgc acgaggaaag
      961 tccccaacag acacaacgag aaggcactga ttgccagcag taatgtccac gctttgaagc
     1021 tggcgcgttg ttcagtgacc gccagtgagt gcatcagcgc agtccccacc agccacggca
     1081 taaacgaggc gttttctacc ggatcccaga accaccagcc accccagccg agttcgtaat
     1141 aggcccatgc ggaaccgagc acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc
     1201 acgggcgagt aaaacgcgca taagtgctgt ccaaacgccc gctcagcaaa gaagcaatgg
     1261 caaaagcaaa cgccaccgag aaacccacgt accccatata aagcagaggc ggatggaaga
     1321 tcagccccgg atcctgtaac agcgggttaa gatcgcgccc ttcaatcggg aagttcggca
     1381 gcgtgcgtga gaatgggtta gaggtaaaga gaatgaacag caaaaagccg acgctgacca
     1441 tccccattat cgccagtacg cgggccacaa tatccagcgg aatacgctga ctaaaaatcg
     1501 ccaccgcaaa ggtccagccg ctcatcagca gcacccacag cagtagcgag ccttcatgcg
     1561 cgccccaggt agccgccacg cgataccaca ccggaagctg ggtattggag ttgctggcaa
     1621 cataggtgac ggtaaagtcg ttgaccacga aagcattgac cagcaccaga aatgcgccag
     1681 ccacagacat aaacagcagc caggcaaaca agcgggaaga cgccatcatg cgcgcatctc
     1741 cgcgcgccac gccccatagc ggatacacgg acagcagcag cgcaatccct aacgccaggc
     1801 acagcagccc gtttccaatt tccggcatca tgatgctggg tccttataaa cactcgccgg
     1861 gcgacggtgg ttagcttcca tcgctttctc aacttctggc ggcgtgtagt tttcatcatg
     1921 tttcgccagc acttcttttg cgaggatatg attgcctttt tccagctcgc cctgcaccac
     1981 aacgccctgc ccttcacgga acagatccgg caaaatgcct tcgtaagaga catccactga
     2041 gccttcagcg tcgtaaatgg tgaacgttac tttcagcgaa ttgggatcgc gctgcacgct
     2101 acccggcatc accatcccac caacgcgcag acgctgaccg acttccggca tttgctgagt
     2161 ttcacgcttg ccgtagagaa tttcccccgg cgtataaaag agatcgatat tcgagcgcag
     2221 cgcatatagc accagaccga tagtcagcgc cagccctgcc aacacggcac aggcaatcca
     2281 caagcggttt ttacggcgaa tattcatgca gcctcctgct gttgcgcagc acgtaaacgc
     2341 gcctcacgcg cccgctgttg cgccacgcca cgcagaattg cgcgatgttg catcaccgag
     2401 tgcacgacca aaaccaccag cggaataacg gtcatcacca ccgccagcca gacaaaaaaa
     2461 gcgtaaccgc ccattgcgaa aaattcattc caggaagcaa atgcaggggt catttacggc
     2521 ctcttttcag tatcagttca ctcacccacg gacggcgttt ttccatcagc aaaatcaaat
     2581 tacgcatccg catcagcgtc agcgtggcag acaggagcag gaagccaaaa atcgaccagc
     2641 gcagcggcga acgcatcgcc ggatcgatac tttgctgcat ccgcgttgat ccctgatgca
     2701 gggtgttcca ccactccacg gagtaatgaa taatcggcag attcaccacg ccaatcagca
     2761 ccaggatacc tgccgcacgg cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa
     2821 tcacacccac atacaaaaac agcagcacca gttcagaagt cagacgtgca tcccataccc
     2881 accaggtgcc ccacatcggt tttccccatg cagagccggt aaccagggca ataaaggtaa
     2941 acacggcacc aatgggggcc atcgccgcca ccgccaggtt ggccattttc atctgccaga
     3001 caaggccaat aaacgccgcc accgccattg atgcataaat gcccatcgac cagatcgccg
     3061 caggtacatg caagtagata atgcggtagc tatttccctg ttgataatca gccggagcaa
     3121 agccgaatcc ccagatccag ccgacggtaa gcacgaccac actggcaatt gccagccacg
     3181 gtataaacca gccacagatt tgatacagcc gtggtgggat cgccagttga tgcagtgttt
     3241 tccacatagt ttcgatacca gactcgaaca aaaatcagta atccagcgtt attgaatgct
     3301 gattcgtaac gctgccgccg tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc
     3361 gcctaaaatt gccagatacc cgtcaacggg caaatgcatg gaagccgcgt ccatcgcggc
     3421 ggtggcaaag atgagtaatg ggatagtcag cggtaacacc agtatgctga gcagcacacc
     3481 accgcgctta agtcccactg tcagcgccac gcccggcgca ccaagaaagc caagcgtagg
     3541 ggttcccagc agtagcgtta acgccatcac ttgccagcca taaacatcca ttcccagtag
     3601 cattgctacc aggggcgaaa ggatgagtaa cggcagaccg gttaccatcc agtgcgccat
     3661 caccttcgcc agcacaacgg cgggcaaggg taacggcaac aacatcaatt gttcaagact
     3721 gccgtcctgc aaatcgtcac ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc
     3781 agcaacccag ataatgcccg gtgcaatacg cgccagcagt tgcggctccg gaccgatact
     3841 gagcggaaaa agggtaatta caatcaggaa gaaccacagc gggttggcga tttcggcgct
     3901 atggcgaaac gctacgcgca gctcaagacg gaaaatgcgc cagaacatca tgcggccctc
     3961 gtttgcgtca gtgaaatgcg gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta
     4021 gtcagaatca caatcccccc ctgctccgta tgctgcgcca tacgctgggt cagacgatcg
     4081 acgccgttaa cgtcaatcgc ggtaaaaggc tcgtcgagga tccataacgt ggcacgggtc
     4141 agccacagac gcgctaaagc gacgcggcgt tgttgcccgg cagaaagttg atttaccgga
     4201 atatcttcga atccggcaag cccggcctgc gccagggctt ccagacattg tgcggtatcg
     4261 ccatcgcgat gataaaagtg cagattttct aacgccgtca gccgggtttt gatccccggc
     4321 tgatggccta tccataacag gttttgatgg tagctgtcgc gtacctgatg caagggctgc
     4381 ccttgccaga gaacctcgcc tgcgtcaggg cgagacaacc ccgtcagcaa acggagaagc
     4441 gttgtcttcc ccgcgccgtt gctaccggtg atttgtaccc actctcctgc gttcagcgta
     4501 aatgataaac cactaaataa ggttcgttca tcccgctcac aaagtaactc tctggcttca
     4561 agcataccca agcaataacc ctgttaaaaa cctggctcga cttcacgcat atcggggagc
     4621 ttgtgcgcta tccctttatg gcaatcaata caggtttgcc catctttcac cgcctggtca
     4681 tgcatcttcg cggcaaccga tttctgggcg gttgtatcca tatactcgaa gttgtgacag
     4741 ttacggcact cctgcgagtt attgtccttc atgcgccgcc actcattctg tgccatcgtc
     4801 aggcgatgag cttcaaattt ctgcggcgtg tcaataacgc caaaaatttt accatacagc
     4861 tctttacttg ctttgagctt gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa
     4921 tccggacagg tcgcacggac gccgctacgg ttgttgtagt gcacggaatc catgtattcc
     4981 tgatacaccg tgttacgcat ttcgtggcag ctaatgcaga actcttcggt attggctttt
     5041 tccatcccag tgttaaagcc gccccagaag acgatgccgc cgacaaaacc gatcaacagc
     5101 agcgtcccca gcgccagacg gctgggggta cgccaccatt tccacaggcg cttaatcaga
     5161 ccaggcttac ggtcagaatt tcccataata acctcttatt tcccgtaacc ttttgatggg
     5221 gtaaaggtat tccccacgat tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag
     5281 aaataacgac gcggagccac ttccgcgccc actttgccgt cgctgtccat aaagtgagta
     5341 ggactgatac gcggtgcgcc agtggtgcga tagctttcga caccgtggca ctgcaagcag
     5401 cggttggtat tggtcgttac ctgataacct tcaacgctat gcgggatcat tggcggctga
     5461 ttcacatagt tcagcggcat ccgatcctgc tcttttggca tccgaatggc cccttcctgc
     5521 gtcccggata cttccggcga ctggctaaaa tcgacgccat tagccgccca aaccgcgccg
     5581 cttaccacca gggccagcat cgccgtccat tgacacagcg ctttcttcag gtcatggctt
     5641 ttcatgattt cgctcccgaa ctccatcgtg tagttattgt aaatacatcc tcagaacaga
     5701 catccacgca gcgaccgcac gtcatgcaat cgcggctggt gacctgcacc gggctttgtt
     5761 catccagcac cggggcacgt agcacatgcg gttccgggca aacatgaaaa caatccatac
     5821 agcggttaca tttctgccga tcgctggccg caacggtaat cacacctttg ctacccagca
     5881 cgccatacag cgcgccaacc ggacaaatgt gcccgcacca gccgtgttca acgaccagta
     5941 gatcaaataa aaacagcgcg agaataagca gcgcgccgct gccgaagccc attaccaggc
     6001 tacgccccat cagggaaacg gggttgatcc attcccagat gagcgtgcca gttaaggctg
     6061 atcccaccag aatgaccacc aacagcacgt agcgaatgtg gcgagggatc gtcgcagact
     6121 gattgaggtc aaagcgcctg cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc
     6181 agacccagct gcaaaataat cgctttcccg ccagggcgta gagcacggtg ataatcaccg
     6241 caccggtcaa cgccaccgtg gcaggcagat gaccactggc aagactttgc agcgtcatca
     6301 gcggatcggt cagcggaacg gtgtcgaata acaggctact gctgtagttg ccgtgcaaga
     6361 tccacacacc aaaccacgga ccgctcaaaa acatccccag cacgaagaac tgacaaaggc
     6421 gacgtaacac cagccaacgg tgactgcgcc accagccttt tttctccagc gcctcgcgcc
     6481 cggcgtcacg tttacgattt gccattgttc ccctccagcc agccgaagcg gtaatggtga
     6541 cctaactccc ctttcgccag tgacagcggt aacaccttga ttgccggttg ttccagcacg
     6601 catacttttt cgcacttacc gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag
     6661 cgggcgtgct taccggtacg cgtgttgcgc tccagctcca gggtgatggc ctcatcaatt
     6721 ttcgggcatt cgcgataaca aacatcgcag cgcagcccct gaaagttgag acagttttcc
     6781 tggtccacca gtaccgccag ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc
     6841 agcgcaccgc tcgggcacac tttggcgcac ggaatgtcct cacacatttc gcaaggaata
     6901 tcccgtgcga caaaatatgg cgtgcccgcc gacaggccag aggccagcgt cgccagtttt
     6961 aaggtgtcgt aagggcaagc ctgaacacac tgaccgcaac gcacacaggc actggcaaag
     7021 gcgttctcgt ttatggctcc gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc
     7081 tgttgtaacc ccagcgccac acccacggca gccagcccgc ctgctgtgcg aacgacatcg
     7141 cgcagaaagc ggcggcgacc attttgaggt ttcgctgacc gggacataat ggcttacacc
     7201 ttctccagtt tgaccgcgca cttcttgaag tccgtctctt tcgagagcgg atcggtcgcg
     7261 tccagcgtta gtttgttaac cagctgtgcg gcgtcgaaga acggcatgta caccaggccc
     7321 tgcggcggac ggttacgacc gcgcgtttcg acaatcgaga tcacttcgcc acggcgagaa
     7381 accactttca ctttgtcgcc acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata
     7441 aacaggaccg cttccgggaa ggcgcggtgc agttccggca cacggcgagt catactgccg
     7501 gtgtgccagt gctccaggac gcgtccggta gagagccaca ggtcgtactc ttcatccggt
     7561 gcttctgccg ccggttcgaa cggcagcgcg aagatcaccg ctttaccatc cggtttaccg
     7621 tagaacttat agccttcgcc cgctttcacg tacgggtcgt taccttcgct gtaacgccac
     7681 tgcgtttctt taccgttcac caccggccag cgcagaccgc gcgctttgtg gtagtcatca
     7741 aacggcgcga gatcgtgacc gtgaccgcga ccaaaccatg cgtactcttc gaacagccct
     7801 ttttgcagat agaagcccag ctcgcgtgat tcatcgttca gctgatcttc cgccagttcg
     7861 gataccggga atttgctcac ttcgggggtg gcatacagaa cttcgtacag cgttttgcca
     7921 cgcagttccg gtttcttcgc cagcagctct tccggccata cttcttcagt tttgaagcgg
     7981 cgggagaact ggactaactg ccagaggtcc gatttcgctt cgcctggcgc ctgtacctgc
     8041 tgacgccaga actgagtacg gcgttcggcg ttaccgtaag cgccctcttt ctctacccac
     8101 attgcggtcg gcaggatcaa gtccgctgcc agcgcactga ctgtcggata cggatcggag
     8161 acgatgatga agttgcgcgg atcgcgccag cccggcatac gctcttcatt aatgttcggc
     8221 ccggcctgca tgttgttggt acacatggtc cagtaaacat tcagcttgcc gtctttcagc
     8281 gcacggtctt gtgccaccgc gtgcagaccg atttttgccg gaatggtgcc gctcgggata
     8341 ttccacttct tctcgcagat atcacgatgt ttctcgttag ttaccaccat gtccgcaggc
     8401 agacggtgag caaaggtgcc cacttcacgc gcagtaccac acgcggaagg ctgcccggtc
     8461 agggagaacg gaccgcaacc cggctgggaa attttgccgg tcagcaggtg caggttgtag
     8521 accaggttgt tggcccacac gccacgagta tgctggttga agcccatcgt ccagtaggag
     8581 atgactttct tgttcggatc ggcatacagc tgcgccagtt gttccagctg atcttttggt
     8641 acgccagtca tttcagcggt tttttccagc gtatactcag caacgaaggc tttgtaatct
     8701 tcaaagctca tcggttcgga ggcgtcagaa cccggattct tcgctgcttt ttccagcgga
     8761 tgggtcggac gtaaaccgta gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgtttg
     8821 ctgaagaagt cctgatttat cgcattgttt tgaatgatat aattggcgat gtagttcagg
     8881 atcaccaggt cagactgtgg cgtaaagatg atgccgttat ccgccagctc gaagctacga
     8941 tgctggtagg ttgaaagcac cgccacggtg acgtcctggt tagagagacg acggttagtg
     9001 atgcgtgacc agaggatcgg gtgcatctcc gccatgtttg caccccacag cacaaacgcg
     9061 tcagcctgct cgatgtcgtc atagcagccc atcggctcat ccataccaaa ggtacgcata
     9121 aagccaacta ctgccgacgc catacagtga cgcgcgttcg ggtcgatgtt gttcgaacgg
     9181 aagcccgctt taaacagctt ggacgcggca taaccttccc agatagtcca ctgaccagaa
     9241 ccgaacatac cgatcgattc cggccctttt tctttcaggg cggttttgaa tttctcttcc
     9301 atcacatcga aggcttgatc ccaggtgatt ggggtaaatt cgccttcttt gtcatattta
     9361 ccgtttttca tacgcagcag cggctgcgtc aaacggtctt taccgtacat gatcttgggc
     9421 aggaaatagc ccttaatgca gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga
     9481 caggccacca cacgcccctg ctgcgttccg accagaacgc cgcaaccagt accgcagaaa
     9541 cggcacggcg ctttgtccca tttaatggct tcctgctgac caacaacggc gcgggcaacg
     9601 cccggcacgc tgagaccggc agccgccgca gcggccgcaa cggcgttagc tttcataaag
     9661 ctacgacgac tgagtttcat ggtgtttcct caccttgctc ttcctgctgg tgataaacca
     9721 gcgacaccgc cagcacgccc tctacgttgc gtactgactc aatggtttgg atcagcgttt
     9781 cgctgtcttc tgcttccacc accacaatca actgaccgct cggcgcgtcg ctgacagcaa
     9841 cttcacagcc gggaaaggcg ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg
     9901 cctgcacgac caggctgcaa acttgccagt tagtgtgcat ggagatactc cgcagttatg
     9961 gctgataccg gacagctggc tgcacatgct ccacatccgt tgcaaagttg gctattaagt
    10021 tgcggctggt agatcccgga gagcgtcggg cgaaagataa ttgccattgg ctcacagcta
    10081 tcctgacagc ggcggcattc aactgactga tacgccaggc acgcgtcccc aatggtgaac
    10141 tgcaaatccc aggccctggt gtggcgcgga gaaaatagcg attcggggca agcctgcgcg
    10201 caggcgtaac agaagctgca ctcgttattt ttgaaattaa cgctcggata gccgcccgcg
    10261 ccgcgttgca gaatgttgtt ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg
    10321 gtcagaaaat gagattcatc accggaccag ggcggacgga taccgttact ggctttgcgc
    10381 cagcgaccag tgagtatgcc ccgacgggat gcatcaatct tcacattgac cttccatcat
    10441 taacgcgctc tgaaaattga gagcgaccaa ataaaccgca taattaataa gccattttta
    10501 tagccgctaa gatattaaag gatgtgtcaa agatgcatac cccgatcgag gtaaaacctg
    10561 tagcaggatc aaaagagtgg cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa
    10621 atggttataa atatatttat atagcgatta attcaccaga gatatttctg ctggtttgct
    10681 ctctcatcag aatttaacac taaaagagca ggtaaaattg tctgaatatt ctttaagtta
    10741 ttcataaagc aaattaataa atctgatgaa tatgttaacc ttcagcgaca tcatcggtgc
    10801 aaacctataa atgaagaagg aaagcaaaaa atgaagacca ttctacctgc agtattgttt
    10861 gccgctttcg ccaccacttc cgcctgggcg gcagagagcg tccagccact ggaaaaaatc
    10921 gcgccttatc cacaagctga aaaaggcatg aagcgtcagg tgattcagtt aaccccgcaa
    10981 gaagatgaat ctaccctgaa agtagaactg ttaatcggtc agacgctgga agtcgattgc
    11041 aatttgcatc gtctcggcgg gaagctggaa agcaaaacgc tggaaggctg gggctatgat
    11101 tattatgtct ttgataaagt tagctcgccg gtttccacga tgatggcctg cccggatggc
    11161 aagaaagaga agaaatttgt caccgcgtat ctgggcgatg ctggaatgct gcgttacaac
    11221 agcaagctgc cgatcgtggt gtatacgcca gacaatgtag atgtgaagta ccgcgtctgg
    11281 aaggcggaag agaaaattga caacgcggta gttcgctaaa ctgccgtgaa gtgcggcacc
    11341 ccgtaggttg gataaagcgg tcacgccgca tccgacatct aac
//
LOCUS       DAPGRN010000134        11281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPGRN010000134 DAPGRN010000000
VERSION     DAPGRN010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.136"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            130..1035
                     /locus_tag="RHW33_003996"
     CDS             130..1035
                     /locus_tag="RHW33_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1647451.1"
                     /translation="MRGKIPKTELLVTFEVVARHESYTRAAEELALTQSAVFRQVSAL
                     EEFLRTPLFSHSKKRIFLNDAGKYYLGIVKETLNKLERDTNTIMTWQPTVQVIELAVN
                     PTFSTHWLIPNLHEFTKLHPDIIVNIHSLANNGDFLNREYDAVIMRENFCAPWAEVEY
                     LFDEEILPVCSGSLLAMSDQKLSVAELLTELPLLHQSTRITGWEEWFALSGVSSPLVN
                     NGPRFDLLSMLIAAVRSNLGVALLPRFAIQHDLDSGDMVIPCDVPIRTGNRFIMTWQE
                     EKSDSPHLQQFREWLLAKSVVPQEM"
     gene            complement(1069..1671)
                     /gene="cobO"
                     /locus_tag="RHW33_003997"
     CDS             complement(1069..1671)
                     /gene="cobO"
                     /locus_tag="RHW33_003997"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV1647452.1"
                     /translation="MEARISTERHVQRQQKLKEQVDTRVAAAIEKKGILIVFTGNGKG
                     KSTAAFGTVTRAVGHGKTVGVAQYIKGQWDNGEYNLLQPLGVEFHIMGTGFTWETQNR
                     QADIDAAKEVWSESKRMLADKRYDLVVLDELTYMLAYHYLDTEEVIASLQNRPAQQSV
                     IVTGRGCHSQILKMADTVSEIRPVKHAFDNGIQAQPGIDW"
     gene            complement(1681..3333)
                     /locus_tag="RHW33_003998"
     CDS             complement(1681..3333)
                     /locus_tag="RHW33_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV1647453.1"
                     /translation="MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPE
                     ALTLLARQAFYEASFFLRSAHLQQVASILNDPQASSNDKYVALQLLRNAEVSAKGVLP
                     NCQDTGTATIVASKGQQIWTGGNDAEALSKGIYSTFQENNLRFSQNAPLDMYTEVNTQ
                     TNLPAQIDISAVAGDEYHFLCVNKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSL
                     GTAACPPYHIAFVVGGLSADQTLKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLL
                     EASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADRNIKAKINKHGIWL
                     EKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMLDLARLPVGTRVSLSGPIVVAR
                     DIAHAKIKARLDSGESMPEYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYIDTF
                     QAAGGSLVMLSKGNRSQQVTDACHKHGGFNLGSIGGAAALLAQEYVKSLRCLEYPELG
                     MEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH"
     gene            complement(3441..4721)
                     /locus_tag="RHW33_003999"
     CDS             complement(3441..4721)
                     /locus_tag="RHW33_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308785.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDV1647454.1"
                     /translation="MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFT
                     TIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITV
                     NFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
                     FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVM
                     VSAGIFMGEFYFTMLLLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFP
                     GTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITM
                     LLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSN
                     AMGAAMVSRWKGEHFGEGCRGKALKPNESNVALP"
     gene            complement(4882..5199)
                     /locus_tag="RHW33_004000"
     CDS             complement(4882..5199)
                     /locus_tag="RHW33_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HDV1647455.1"
                     /translation="MKQSIVSLAQVIRSKNAGPYELVLDILFKTKENYERVKSSGQLT
                     PELIARLYHVEPDFIHRIVWFDPSNAVKIVMPRDIISGNVGDNDVYGAQQHAPLLNMS
                     FDL"
     gene            complement(5196..6566)
                     /locus_tag="RHW33_004001"
     CDS             complement(5196..6566)
                     /locus_tag="RHW33_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HDV1647456.1"
                     /translation="MARSFKVLSPTAILGYGFPEESFRKAMAESPDLIAVDAGSSDPG
                     PHYLGAGKPFTDRAGVKRDLRYMITAGVQNNIPVVIGTAGGSGAAPHLEWCREIIHEI
                     AQEEHLSFSMALIPADVDKAIVHQALDNGKITALDFVPPLTHDAIDESTYIVAQMGIE
                     PFQRALKEGAQVVLGGRAYDPACFAALPIMQGFDEGLALHCGKILECAAIAATPGSGS
                     DCAMGIIDDNGFTLKTFNPKRKFTETSAAAHTLYEKSDPYFLPGPGGVLNLKGCTFKA
                     VNDGEVYVSGSKHEETPYALKLEGARRVGFRCLTIAGTRDPIMIAGIDKIIDEVKTSV
                     SRNLSLDDDSIRINFHLYGKNGVMGDHEPMQTAGHELGIVLDVVAPTQEIANSVCSLV
                     RSTMLHYGYENRIATAGNLAFPFSPSDIQGGPVYEFSIYHLIEANDALRFDFHIEQVT
                     PEGVQA"
     gene            complement(6570..7811)
                     /locus_tag="RHW33_004002"
     CDS             complement(6570..7811)
                     /locus_tag="RHW33_004002"
                     /EC_number="4.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate ammonia-lyase"
                     /protein_id="HDV1647457.1"
                     /translation="MKIKQALFTAGYSSFYFDDQQAIKNGAGHDGFFYTGEPVTQGFN
                     AVRQAGECVSVQLILENGAVAVGDCTAVQYSEAGGRDPLFLAEHFIPFLNDHIKPLLV
                     GRDVDAFLPNARFFDKLRIDGNLLHTAVRYGLSQALLDATALATGRLKTEVVCDEWQL
                     PRVAESIPLFGQSGDDRYIAVDKMILKGIDVLPHALINNVEEKLGFKGEKLREYVRWL
                     SDRILSKRTSARYHPTLHIDVYGTIGLIFDMDPLRCAQYIASLEKEAQGLPLYIEGPV
                     DAGNKPDQIRLLTAITKELTRLGSGVKIVADEWCNTYQDIVDFTDAASCHMVQIKTPD
                     LGSIHNIVDAVLYCNSHSMEAYQGGTCNETDVSARTCVHVALAARPMRMLVKPGMGFD
                     EGLDIVFNEMNRTIALLQAKD"
     gene            complement(7811..9256)
                     /locus_tag="RHW33_004003"
     CDS             complement(7811..9256)
                     /locus_tag="RHW33_004003"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit E"
                     /protein_id="HDV1647458.1"
                     /translation="MELRNKKLTHDEFMTERQQVLKTWETGKDVEIFEDGVKYQQTIP
                     EHKRFSHALLKADKEGKTLSQPRAGVALMDEHIELLKTLQEECDLLPSTIDAYTRLNR
                     YEEAAVGIKKSIEAGTSKLNGLPVVNHGVAACRRLTETLQKPLQIRHGTPDARLLAEI
                     SMASGFTSYEGGGISYNIPYAKRVTLEKSIRDWQYCDRLMGMYEEHGIRINREPFGPL
                     TGTLIPPFISHSIAIIEGLLALEQGVKSITVGYGQVGSLTQDVAAIQSLRELAHEYFQ
                     SYGYTDYELSTVFHQWMGGFPEDESKAFAIISWGAAVAGMSGATKVITKSPHEAWGIP
                     TAAANIQGLKASRQMLNMVNEQKFPPCPAVELEIELIKSEVRAVLNKVFELGNGDIAR
                     GTVLAFEAGVLDVPFAPAACNAGKILPVRDNTGAIRVLEAGAVPLPKDILDLHHDYVA
                     ERARCEGRQPTFQMVVDDINAVSHSKLIGRP"
     gene            complement(9275..10663)
                     /locus_tag="RHW33_004004"
     CDS             complement(9275..10663)
                     /locus_tag="RHW33_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDV1647459.1"
                     /translation="MQIVSVDIGSTWTKAALFTREGDALTLVNHVLTPTTTHHLAKGF
                     FSSLNQVLNVDNALPLLNNGEVALKYSSSAKGGLAVAAMGLVPSITLETAKVTAHSAG
                     AKIAQYYAYKLNRRDIQALEETQPDILLFTGGTDGGEESYGLNNARVLAESKLDCAII
                     YAGNRDIQDEVQEILGHKDLTIVDNVLPDLDHPNPLAARQAICDIFLKRIVKGKGLDV
                     IVDKTGEEPMPTPWTVFELVKAISNVDHSWKEFMLIDMGGATTDVYSACANTLSPDTV
                     LHGVPEPFVKRTVEGDLGMRVSAVVVGESAKELVNVNFAQQPAQLDAFYHYLRHLVAH
                     PDYLPANAEEKYFDTVLAGLCVGYATERHAGTKKEVCTCVGNVDLQMGRDLTTVRKVV
                     GSGGWLSRASQFDMHHWLKYRELNDDGKRILLPTEFEYYRDSRGLLPLLANVARVDPL
                     AAARTSIQCLTL"
     gene            complement(10663..11112)
                     /locus_tag="RHW33_004005"
     CDS             complement(10663..11112)
                     /locus_tag="RHW33_004005"
                     /EC_number="5.4.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylaspartate mutase subunit S"
                     /protein_id="HDV1647460.1"
                     /translation="MKKATLVIGVIGADCHAVGNKVLDRVFSNHDFRVINLGVMVSQD
                     EYIDAAIETGADAIVVSSIYGHGDIDCLGMRERCIERGLGDILLYVGGNLVVGKHDFA
                     DVENKFKEMGFDRVFAPSHDLEEVCQLMAHDINQRHDVDTRILEEAI"
BASE COUNT         2678 a         2833 c         2829 g         2941 t
ORIGIN      
        1 cgccagcctt actggtttat aaatgtcaat catgattctt ataaattatt tattgctgct
       61 ttggttacca ttacttcgta ataagtaatg tatttttgat ggtttaaact atttttaagg
      121 gacggcatta tgcgagggaa aatacctaaa acggaactgc tggtgacttt tgaggtcgtc
      181 gcacgtcatg aaagttatac cagagcggca gaggagttag cgttaaccca aagtgcagta
      241 ttcagacaag taagtgcgct ggaggaattt ttacgtactc cgttattttc acactctaaa
      301 aaacgcattt ttcttaatga tgcagggaag tattacctgg ggatagtaaa agaaacatta
      361 aataagctgg aacgcgatac caataccatt atgacctggc agcctacggt tcaggttatt
      421 gaacttgccg ttaatcctac ttttagtacc cactggttaa tcccaaattt acatgaattc
      481 acaaagttac atcccgatat tattgttaat attcattctt tagctaataa cggcgatttt
      541 cttaatcggg agtacgatgc tgtcattatg cgcgaaaatt tttgcgcgcc ctgggcggaa
      601 gtagaatatc tctttgatga ggagattttg ccggtatgca gcggcagttt gcttgcaatg
      661 tccgatcaaa aactgtcagt ggcagaactg cttactgagt taccgcttct gcatcaaagc
      721 acccgtatta ctggctggga agagtggttt gctttatctg gcgttagcag tccgctggtc
      781 aataacggac cacgtttcga tttgctttca atgctgattg ccgccgtgcg ctctaatctc
      841 ggcgtggcgc tgttaccacg tttcgctatt caacatgatt tggatagtgg cgatatggtg
      901 attccctgtg atgtgcctat tcgtaccggt aatcgtttta ttatgacgtg gcaggaagag
      961 aagtctgact caccgcactt gcagcaattt cgtgaatggt tgctggcgaa atcggtcgta
     1021 ccgcaggaga tgtaaaacct gcgcctggcg ggatgccagg cgcagttatt accagtcgat
     1081 acccggctgg gcctgaatgc cgttatcaaa agcatgtttg acggggcgga tttcgctgac
     1141 cgtatctgcc atttttaata tctgactgtg gcaaccgcgt ccggtgacaa tgacactttg
     1201 ttgtgccggg cggttttgca gagaagcgat cacttcttcg gtatccagat aatggtaagc
     1261 cagcatatag gtcagctcat caagcaccac taagtcgtaa cgcttgtcag ccagcatccg
     1321 tttgctttcc gaccagacct ctttcgccgc gtcgatatcc gcttgccggt tctgtgtctc
     1381 ccaggtaaaa ccggttccca taatatgaaa ctcaaccccc agcggctgga gaaggttgta
     1441 ttcgccgttg tcccattgcc ccttgatgta ctgcgcaacc cccaccgttt tgccatgacc
     1501 caccgcgcgc gtcacagtac caaacgcggc agtggatttc cctttgccgt tgccggtgaa
     1561 aacaatcaag atcccttttt tctctatcgc cgctgcgacg cgcgtatcga cctgttcctt
     1621 caatttctgt tggcgctgca catgccgttc agttgaaatt cgcgcttcca ttcattttcc
     1681 ttaatgtccc gcagggcaac tggcgcagcg atgttgctgc tcaaactgac taaagaaatt
     1741 attgccttta tcatctacca ggatgaatgc gggcagattt tccacttcca tcatccacac
     1801 cgcttccata cccagctccg ggtattccag gcaacgcaga cttttgacat attcttgcgc
     1861 cagtaacgca gctgcaccgc caatacttcc cagattgaag ccgccatgtt tgtgacaggc
     1921 atcggtgacc tgctggctgc ggttaccttt cgataacatc accagactgc cgccagccgc
     1981 ctggaaggta tcgatataac cgtccattcg accgcccgtg gttgggccca gtgaaccgca
     2041 ggccatgttc tccggtgttt tcgccgggcc agcgtaatag acaatatgat gcttcaggta
     2101 ctccggcatc gattcgccgc tatccagccg cgcttttatt ttggcgtggg cgatatcgcg
     2161 cgccactacg atgggaccac ttaacgacac gcgggtgcct accggtaatc tggcgagatc
     2221 cagcatcacg tcacgcaacg gacggtttag atccagttgt acatgttgcg cgtgattttc
     2281 ttcccgcagt gaggctggaa tatattgccc cggattgtgt tcgagttttt ccagccagat
     2341 accgtgtttg ttaatttttg ctttgatatt gcgatcggca gaacaggaga gcgccatagc
     2401 tatcgggcaa gagccaccgt gacgcggcag acgaatgacg cgaatatcat gagcgaaata
     2461 tttaccccca aattgcgcac caataccaaa ttgctgactg gcctcgagca acactttttc
     2521 cagctcaata tcacggaatg cttgtccctg ctcgtttccg ctggtgggca aattgtcgta
     2581 atatttcgtt gaggccagct tggcaacttt taaggtttga tcggctgaaa gccctccgac
     2641 gacaaaggcg atgtggtaag gcgggcaggc agcggtgccc agcgatttca ttttttcgat
     2701 taaaaatgcg gtcagttttt ctggttgcaa taaggatttg gtttcctgat aaagcgcggc
     2761 tttattggcg gagccgcccc ctttattaac acagagaaaa tggtactcat caccagcgac
     2821 agcgctgata tcaatctgcg ccgggagatt ggtttgcgta ttgacttcgg tatacatatc
     2881 cagcggcgca ttttgtgaaa agcgcagatt attttcctgg aaagtactgt aaatgccttt
     2941 gcttaaggct tcagcgtcgt tacctcccgt ccatatctgt tgccctttac tggcaacaat
     3001 agttgcggtg ccggtatcct gacagttggg taatacccct tttgctgaga cttcagcatt
     3061 acgtaataac tgcaacgcaa cgtatttatc attactgctg gcctgcggat cgttaagaat
     3121 gctggcgacc tgttgcaaat gagcggaacg taagaaaaaa gaggcttcat aaaatgcctg
     3181 ccgggcaagc agcgttaatg cttcaggagc gatttttatc acttcttcgc cgtctaattc
     3241 cgttactgta atatgctgat cgctaattaa tgtatattct gtaccgtctt tattttgtag
     3301 aaacggttct tgccagataa atggtttgga catatttcac ttttcacctt aaacgttagt
     3361 gttacttaat tgccgtctaa cttcatgatc cggcttataa aaatgcgtag tcattggctt
     3421 ctgattgctt aagatccccc tcagggcaga gcaacgttcg attcattggg ttttaatgct
     3481 ttaccccgac agccctcgcc gaaatgttcg cctttccacc gactcaccat cgcagcgccc
     3541 attgcgttgc tcatgacgtt tgtcgcggaa cgccccatat caaggaacgg atcaacgccc
     3601 atcagcaaga tcagccccgc ttccggaata ttgaactggt tgagcgtggc ggcgataacc
     3661 accattgagg cgcgtggtac gccagccatt cctttcgagg tcaacatcag gatcaacagc
     3721 atggtgattt gctcaccgat ggataaatgg atattgcagg cctgggcgat gaaaactgtg
     3781 gcgaaggagc agtaggccat tgatccaacg agattaaatg agtagccaat gggtaagaca
     3841 aagctggcaa ttttggggga aacgccaaat tgctccagtt tttcaagcgt tcccggaaaa
     3901 gccgcttcag aactggatgt ggtaaatgcc agcagggcgg gttccgaaag ggcgcgggtc
     3961 aggcgtctga tgcaggggcc gacataaacg atggccagac cgataagcag cacccaaagt
     4021 aataacatgg tgaaataaaa ttcacccata aagatcccgg cgctcaccat aactgccagt
     4081 cctcgttcag caatcaatgc tgaaatagca gcgaatacgg tcaggggagc gaagagcatg
     4141 acgtagccag tgagctttaa catggcatgt gccagcgaat ctaaggcgtg aacgatggcg
     4201 ctgcctttct cgccaatcgc cgtcaggcta cagccgagga aaattgagaa caccacgatt
     4261 tgcaaaattt cattgtgcgc catggcatcg acaatgctgg tggggaaagc atgcgaaata
     4321 aatactttta gcgtgaaggg ttctgacgcg accactccgg tggtttcggc accgtgtgca
     4381 acaaaattaa ttcctgtgcc tggcatgaag aaatttactg ttatcaagcc taaggcaatt
     4441 gacagcaatg aggcgcaaat aaataaaaag agtgttttag aaaaaatacg accaagggct
     4501 ttggcatcgc ccattttagc aatacctacc accagggtag aaaccactaa cggagcgata
     4561 atcattttta tcagtcgtaa gaaaatcgtc gtgaatatcg atatttcttg cgcatagagt
     4621 tttgctgttt ccggtgaagc agtgttattg agcactacgc cgataatcat tccaagtacc
     4681 agcgccaaaa gaatcatcgt ggttaaactt attttcttca ttttttcctc catcaggtgc
     4741 acggcgataa atcagaatca ggagaagtag atccctgaat agagattcag gcatctacct
     4801 gctattacaa ccttacaaat agctgaagtg ttgaactgca ataagtgacg ggatgtcagt
     4861 tggctaaaag tattcggatt tttacaaatc gaaactcata tttaataaag gtgcatgttg
     4921 ctgtgcacca tagacatcat tgtcacctac attcccggag ataatatctc gtggcatgac
     4981 gatttttact gcgttagaag gatcaaacca gacaatacga tgaataaagt caggttcaac
     5041 atgatataag cgcgcaatca gttctggggt taattgccct gatgatttaa ctcgctcata
     5101 gttttctttc gttttaaata aaatatctaa aaccagttcg taaggtccgg catttttcga
     5161 acgaatgact tgcgccagtg agacaattga ctgtttcatg cctgaactcc ttctggcgtc
     5221 acctgttcaa tatggaaatc aaaacgcaga gcgtcgttgg cttcaatcag gtgatatatg
     5281 gaaaattcgt ataccggccc gccctggata tcagaagggg agaacgggaa cgccagatta
     5341 cctgcggtag cgatgcggtt ttcatagccg tagtgcagca tggtagagcg caccagcgag
     5401 caaacgctgt tggcaatttc ctgggtcggt gcaactacat caaggacaat ccccagctca
     5461 tgcccggcag tttgcatcgg ttcatggtct cccatcaccc cgtttttgcc gtacaggtgg
     5521 aaattgatgc gaatgctgtc atcgtcgagc gacaggttac gcgaaacgct ggttttgact
     5581 tcatcgatga ttttatcgat cccggcgatc ataatcgggt cgcgcgttcc ggcgatggtc
     5641 agacagcgga agcccacccg tcgtgcacct tccagtttca gggcatacgg cgtttcttca
     5701 tgcttagaac cgctgacgta gacttcgccg tcattgactg ctttaaatgt gcagcctttc
     5761 aggttcaaca cgccgccagg tcctggcagg aagtagggat cggatttctc atacagtgtg
     5821 tgtgcagctg ctgacgtttc ggtaaattta cgcttcggat taaatgtctt cagcgtaaag
     5881 ccgttgtcat caatgatgcc catcgcacag tcagagcctg agccgggcgt tgcggcgatt
     5941 gccgcgcatt caaggatttt cccacaatgc agcgccagac cttcatcaaa gccttgcatg
     6001 atcgggagcg cagcaaagca ggccgggtcg taagcgcgcc cgcccagcac cacttgcgcg
     6061 ccttctttca gcgcccgctg gaagggttcg atgcccattt gcgcgacgat atacgtactt
     6121 tcgtcaatcg cgtcgtgggt taacggcggg acaaaatcca gcgccgtgat tttgccgtta
     6181 tccagcgcct ggtggacgat ggctttgtca acgtctgccg ggatcaacgc catcgagaac
     6241 gagaggtgct cttcctgggc aatctcatga attatttctc ggcaccactc caggtgtgga
     6301 gcggcaccag aaccgccggc cgttccgatc accaccggta tattattttg cacgcctgcg
     6361 gtgatcatat agcgcagatc gcgcttgacc cctgcgcgat cggtaaacgg tttgcccgct
     6421 ccgaggtagt gggggccagg atcggaagag cctgcatcaa cggcgatcag atcgggcgat
     6481 tctgccatcg ctttgcggaa gctctcttcc gggaatccat acccgaggat tgccgttggc
     6541 gataagactt taaaagaacg agccatttgt taatccttag cctgcaacag cgcgatagta
     6601 cgattcattt cgttgaagac gatatcgagg ccttcgtcaa aacccatccc tggttttacc
     6661 agcatacgca tagggcgagc ggcaagggcg acgtggacac aggtgcgggc actaacatca
     6721 gtttcattgc aggtgccgcc ctggtacgct tccatgctgt ggctgttgca gtaaagaacc
     6781 gcatcgacga tgttgtgaat actgcccaga tccggggttt tgatttgcac catgtgacaa
     6841 ctggcagcat cagtgaaatc aacaatatcc tggtaggtgt tacaccattc atcggccaca
     6901 attttcacgc cggaaccgag gcgcgtcagc tctttggtaa tcgcggtcag caggcgaatt
     6961 tgatcgggct tgttaccggc atcgaccggc ccttcgatgt agagcggcag gccttgcgct
     7021 tctttttcca ggctggcgat gtattgcgca cagcgaagcg gatccatatc gaagatcaga
     7081 ccgatagtgc cgtatacatc gatgtgcagg gttgggtggt agcgtgcgct ggtgcgcttg
     7141 ctcagaatgc gatccgacaa ccagcggaca tattcgcgca gtttttcacc tttaaagccc
     7201 agcttctctt cgacgttatt aatcagcgca tggggcagca cgtcgatgcc tttaaggatc
     7261 atcttatcga cggcgatata tcgatcgtcg ccgctctgac caaataatgg aatggattcc
     7321 gccacgcgtg gcaactgcca ttcatcacag accacttcag ttttcagacg gccggttgcc
     7381 agcgcggtag catcaagcag cgcctgtgat aatccgtagc gcacggcggt atgcagcaag
     7441 ttgccgtcaa tacgcaattt gtcgaagaaa cgggcattcg gcaggaaagc atccacatcg
     7501 cggcctacca gtaatggctt gatatggtcg ttgaggaacg gaataaagtg ctctgcgagg
     7561 aacagtggat cgcgaccgcc tgcctcggaa tactgtacgg cagtacagtc acctacggcg
     7621 accgcgccgt tttccagaat caactgtacc gatacgcact ccccggcctg acgaacggcg
     7681 ttaaacccct gtgttaccgg ctccccggta taaaaaaagc cgtcatgacc cgctccgttt
     7741 tttatcgcct gctggtcatc gaaatagaat gaggagtagc cagcggtgaa cagagcctgt
     7801 tttattttca ttatggtctt cctattaatt tactgtggga tacagcattg atgtcatcta
     7861 caaccatctg gaatgtcggt tggcgtcctt cacagcgtgc gcgttctgca acgtaatcgt
     7921 ggtggaggtc gagaatatct tttggcaatg gaacggctcc ggcttccagt acgcgtatcg
     7981 cgccggtgtt gtcacggacc ggaagaattt tgcccgcgtt gcaggcggct ggcgcaaacg
     8041 gcacatccag tacgcccgct tcaaatgcca gcaccgttcc gcgcgcaata tcaccattac
     8101 ccagttcgaa cactttattc agtactgcgc gaacctcgct cttaatcagt tcaatttcca
     8161 gttcaaccgc agggcacggc gggaatttct gctcattgac catgttgagc atctggcggg
     8221 aggctttcag cccctgaatg ttggcagcgg cagtcgggat accccaggct tcgtgcggac
     8281 ttttggtgat gactttggtg gcaccagaca tccccgcgac ggctgcaccc caggagataa
     8341 tagcgaacgc tttcgattcg tcttccggaa agccgcccat ccactggtga aacacggtac
     8401 tcaattcata gtcggtatag ccatagctct gaaaatattc gtgtgcaagt tcacgcagag
     8461 actggatcgc cgcaacatcc tgcgtcaggc tgccgacctg gccataacca acggtgatag
     8521 atttcacacc ctgttccagc gccagcaagc cttcgattat cgcgatagag tgcgagataa
     8581 acggcgggat cagcgtaccc gttagtgggc caaacggttc gcggttgatg cggatcccgt
     8641 gttcttcgta catccccatt aaacggtcgc agtactgcca gtcgcggatc gatttttcca
     8701 gcgttacacg tttggcatac ggaatgttgt aggaaatgcc accgccttca taactggtaa
     8761 agccactggc catcgagatt tcagccagca gacgcgcatc tggcgtgccg tggcggatct
     8821 gtagcggttt ttgcagggtt tcggtgaggc gacggcaagc tgcgacgccg tggttaacta
     8881 ccggtaaacc gttcagttta gaggttccgg cttcgatgga ttttttaatc cctacagccg
     8941 cttcttcgta ccgattcaga cgggtatatg catcgatagt gctcggcagc agatcgcact
     9001 cttcttgcag tgttttcagt agttcgatgt gttcgtccat cagcgccaca cctgcgcgcg
     9061 gttggctaag ggttttacct tccttgtctg ctttcagcag ggcatgagag aagcgtttat
     9121 gctcagggat ggtttgttgg tatttaacgc catcttcaaa gatttcaaca tccttgccgg
     9181 tttcccaggt tttcagtacc tgttgccgct cggtcatgaa ttcgtcatgg gttaatttct
     9241 tatttcgaag ttccattgat tgcagcctta attgttatag ggttaagcat tgaatactgg
     9301 tgcgcgcggc ggccagcgga tcgactctgg cgacgtttgc cagcagtggt aatagccccc
     9361 gggaatcgcg gtaatattca aattcggtcg gtaagagaat gcgtttaccg tcgtcgttta
     9421 actcgcggta tttcaaccag tgatgcatat cgaactgact ggcacgcgag agccatccgc
     9481 cggatccgac gactttacgc acggttgtca ggtcacgtcc catctgtaaa tcgacattgc
     9541 cgacgcaggt gcagacctct tttttggtgc ccgcatgtct ttcggtggca taacccacac
     9601 acagtccggc caggacggta tcgaaatatt tctcttctgc atttgcgggt aagtagtcgg
     9661 gatgagcgac tagatgacgg aggtaatgat aaaaagcgtc cagctgtgcg ggttgctgag
     9721 caaaattcac attgactaac tctttcgcac tttcaccaac caccactgcc gagacgcgca
     9781 ttcccagatc gccttccacc gtccgtttta cgaagggttc cggtacgccg tgtaaaacgg
     9841 tgtcgggcga gagcgtgttg gcacaggcgg aataaacatc ggtggttgcg ccgcccatgt
     9901 cgatcagcat gaactctttc cagctgtgat cgacgttact gattgccttg accagttcaa
     9961 agaccgtcca tggtgtcggc attggttctt cgcctgtttt atcaacaatg acatccagcc
    10021 ctttcccttt aacgatgcgt ttaaggaata tgtcgcaaat agcctggcgt gcggctaaag
    10081 ggttcggatg gtcaagatcg ggcagaacat tgtcgacgat ggtcaggtct ttatgcccga
    10141 ggatctcctg cacctcgtcc tgaatatccc ggtttccggc ataaataatg gcgcagtcga
    10201 gtttggattc tgccagcaca cgggcgttat tcagtccgta gctttcttct ccgccgtcgg
    10261 tgccgccggt gaacaagaga atatcgggct gcgtttcttc cagtgcctgg atatcacggc
    10321 ggttcagctt gtaggcgtaa tattgcgcaa ttttagcacc cgcagagtga gcggttactt
    10381 tcgcggtttc cagggtgata gaaggcacca atcccattgc ggcaaccgcc agcccacctt
    10441 tggcagagga ggaatacttc aatgcaacct caccgttgtt taacaggggg agggcattat
    10501 ctacgtttaa cacctggttc aggctggaga aaaacccctt cgccagatga tgggtggtgg
    10561 ttggggtcag tacgtggtta accaacgtta acgcgtcccc ctcccgggtg aagagggctg
    10621 ctttggtcca cgtcgatcca atatcgacag agacgatttg catcagattg cctcttcaag
    10681 aatgcgagta tcaacgtcat gacgttggtt gatatcgtgg gccatcagct gacaaacctc
    10741 ttctaagtca tgacttgggg caaatacgcg atcaaatccc atctctttga acttgttttc
    10801 cacgtcagcg aagtcatgtt ttcctaccac caggttaccg cccacataga gcaggatgtc
    10861 ccccagtcca cgctcgatgc agcgttcgcg catgcccaga cagtcgatat cgccatgtcc
    10921 atagatagag gagacgacaa tcgcgtcagc gccagtttca atggcagcat caatatattc
    10981 gtcctggctg accattacgc ccagattaat gactctgaag tcgtggttgc taaaaacgcg
    11041 gtccagaact ttattgccta ctgcatggca gtccgcgcca ataacgccaa tcacaagtgt
    11101 tgctttcttc atgggtaatc ctttgtaaga cagagagtaa aataagttac acacgtcgta
    11161 tttatggccg ttcataacgg cacaggttta atatcacagg agtgtcttta tctgaccgta
    11221 atgttgcaat tgatctattt tcaaaatgtc tttcgggaaa tgattattta aagtgtgttt
    11281 t
//
LOCUS       DAPGRN010000135        11156 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPGRN010000135 DAPGRN010000000
VERSION     DAPGRN010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.137"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(153..419)
                     /locus_tag="RHW33_004006"
     CDS             complement(153..419)
                     /locus_tag="RHW33_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV1647461.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTEAIYCQQGSDWQCVVHYDAEASTRQ"
     gene            complement(416..1183)
                     /locus_tag="RHW33_004007"
     CDS             complement(416..1183)
                     /locus_tag="RHW33_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647462.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDCITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLRMPVNAHPDAQFAGAIGAAVIGQRVRTRR"
     gene            complement(1193..2344)
                     /locus_tag="RHW33_004008"
     CDS             complement(1193..2344)
                     /locus_tag="RHW33_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV1647463.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVYVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKETLINE
                     LDAMTARIRQQWEESQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLKMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            complement(2460..3740)
                     /locus_tag="RHW33_004009"
     CDS             complement(2460..3740)
                     /locus_tag="RHW33_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV1647464.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            complement(3781..5013)
                     /gene="mdtM"
                     /locus_tag="RHW33_004010"
     CDS             complement(3781..5013)
                     /gene="mdtM"
                     /locus_tag="RHW33_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV1647465.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEEQ"
     gene            5480..6424
                     /locus_tag="RHW33_004011"
     CDS             5480..6424
                     /locus_tag="RHW33_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV1647466.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDDKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLIRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYNAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLAVLLVTGCANDSQITALLNYIL
                     LTGDEARFNEFISELTRRMPQHRERIMTIAERIHNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQRYTGLSAEQMQALEQPLPESKRDPWIEY"
     gene            complement(6666..8078)
                     /locus_tag="RHW33_004012"
     CDS             complement(6666..8078)
                     /locus_tag="RHW33_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV1647467.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSMALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            8255..8419
                     /locus_tag="RHW33_004013"
     CDS             8255..8419
                     /locus_tag="RHW33_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV1647468.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            8516..10609
                     /locus_tag="RHW33_004014"
     CDS             8516..10609
                     /locus_tag="RHW33_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDV1647469.1"
                     /translation="MKATEARLLDFLKRSQQFVIPIYQRTYSWTGQQCRQLWDDIIRA
                     GKRDDISAHFIGSVVYIEQGLYQVSGISPLLVIDGQQRLTTAMLLIEALSRHLGEDEV
                     FDGFSAMKLRNYYLLNPYESGEKGFKLLLTETDKDSLLALIKQRPMPENYSHRIMENF
                     TFFDEQITKLGDDLIPLCRGLAKLLIVDVALNRGQDNPQLIFESMNSTGKALSQADLV
                     RNFILMGLEPEHQTRLYEDHWRPMEVAFGQQGYSEYFDSFMRHYLTVKTGEIPRTDEV
                     YEAFKLHARSQSVAEKGVDRLVEDIHIYAEYYCAMALGKESDKLLATAFQDLRELKVD
                     VAYPFLLALYHDYKNGVLSHEDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVI
                     NKGKYLESIQVHFLNLPSYRRFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRER
                     VEEFTIEHIMPQNENLSAKWREELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAE
                     KRDIEDGFKHSPLYLNIGLGQCEKWDEAAIHARADRLADLAVQVWQAPSLPEEVLAVY
                     RGQPENKTSYSLSDYPFLADGSHSRVLFDHLSDEVMRLDAGITQEVLKLYIAFKAETN
                     FVDVVPQKSRLRLSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMG
                     LIRQAFEKQMESALI"
     gene            complement(10656..10997)
                     /gene="symE"
                     /locus_tag="RHW33_004015"
     CDS             complement(10656..10997)
                     /gene="symE"
                     /locus_tag="RHW33_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV1647470.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPADEESELMQSLRKVCKLSARKQKQVQEF
                     IGVIAGKQKVA"
BASE COUNT         2899 a         2802 c         2822 g         2633 t
ORIGIN      
        1 atattgtatt ttagacgcgc aataggaagg tgctatcaaa aatcgctcat tttgtggatg
       61 ggaaatattt cacggcaggc gagatattta ctacaacttc cttcatgcag gattatgcgc
      121 agccaattat ccggttggta tatttaaaga tcttattgcc tggttgacgc ttctgcatcg
      181 taatgcacca cgcactgcca gtcgctgccc tgctggcaat atatcgcttc ggtttgccca
      241 gggatcaccc attgtatttc ctcgccgggg ggacaggtca gccaaatgca taacccgcag
      301 ccgccgcgta aatcacgggg aatatcgctg acgcgaaaag tcatgcccgc tgcctgcaac
      361 gccttgcggg tttgtatgac gccgaccgtg gagtgaaata aaaataaaaa ctctttcatc
      421 ggcgtgtcct cactcgttga ccaattaccg ccgcgccaat tgcgccagca aattgcgcat
      481 caggatgggc atttaccggc attcgcaggt gagattccag catccgggca aacgtctggc
      541 aatgactaac gccaccagta aacagaatcg gcgcttcaca ggagagacga gcaatgaaat
      601 tggcactccg ccgcgccatc gcgttaatca ctccggcgag aatcgcttct ggcgcgacgc
      661 ccgctgagcg caggctgatc acttctgatt cagcaaacac tgtgcacata ctcgtgatgg
      721 cgtgcggcgt gacattttcg gtaatgcagt cgagttgctc gacgctggtg ccaagcgtgc
      781 gcgagatcac ctccaggaaa cgcccggtgc ccgccgcgca tttgtcattc atcaggaaat
      841 cgcacaggtt accgtcatca tcaagctgaa tcactttgct gtcctgacca ccgatgtcga
      901 ttaccgcgcg cgttgctggc gcaagaaacc gtgcgcccag cccgtgacag gagatttccg
      961 tcacttgttt atcggcaaaa tccaccagtt gccgcccgta gccggtgagc gtcagaaacg
     1021 gcgttgtctc taacccttcg cgcagagttt cccaggcttc agtaattgct gtcgccggac
     1081 gaaagggtgt tggaacgagg aaacggcgcg taatcacgcc gtctgccagt aagatccctt
     1141 tggtggcggt agaaccagaa tcaatgccaa tcgaatatgt cactgctact ccttacagca
     1201 tctcaataaa ggccgcgaca cgggtactga gctgcccaac atccgaggtg gagtagtctg
     1261 tttcaatagc gatataagga atgttgtgct gctgacgcac atgacgttta attgccagcg
     1321 attccaccgc gtaggtatgg cacgcctgca aaatcacatc aactacgcca tcgacctgat
     1381 attcttccac catctggctg agcattttca ggcgctgatc gttcggcgaa acacaggagc
     1441 agccaatcgc cagatattta tccgccagcg cgtcgtagac atcgcccgtt tctgccacgc
     1501 attgctcggt cgctttcgcc ccggtgcagt tttcataacc gacaacccag ccgccattct
     1561 cttcaatcgc gcgcaccact ttctctgctg cgccgccaat cgggcagccg gtgattaaaa
     1621 tgcgcggacg cgggtccagt cgctggcttt cttcccactg ctgacgaatg cgggcggtca
     1681 ttgcatccag ttcattgatc aacgtttctt tgtcaaaacg gaaggttgcg ccgtagacca
     1741 ctttcagaat gtcgctgccg ctaagcgccg gaggatttaa ctgcccaaga tgataaaaat
     1801 tggccagtgc gcgacgttcg cggtttttca gcgcaatggc atcgcgcaga gcatcttcgc
     1861 taatctcgtg cccaaaacgt tcttctaccg ttttttgcaa gcgcagcatc tcggctttcc
     1921 acaatgcacg cgaggcatcg tccttaacgc tgttcggcag ctgcatcaca taaacaggct
     1981 taaactccgc catgtattca tacattttct ttttgccgtc gcaggtggtt tcaccgacca
     2041 ccagatccga aaagtagaag taggggcatt tatcggtttt gccgaagccg tagctgcttt
     2101 taatcagcgg gcagaggttg cgcggcagat ctttctccgc ttcttcaatg gtttcatcag
     2161 aggtggaaca gagcgaaacc acaaccgcac cggctgccat cgggatctct tgcggcataa
     2221 aggtgcagta agtgccaacc agcggaatgc cgcgctcctt gagatccatg acggtgagaa
     2281 agcctttctg gcgagcttca gagaactgat cgaaaatggc gggtagatcg gtgacaagtg
     2341 acatgatttt ccttccccgt accacgggag ataatgaaaa gaggccacat tattaccatt
     2401 cttttagtat ggtttatttg atctctctcg cggttagcca cttagttttt catggatttc
     2461 taaaaattgc cgagggggaa caaggccggg agctgtgaca gcaaatataa aattagccca
     2521 atacaaccgc caaccagcgt accgttgaca cggataaact gcagatcttt gccgatattt
     2581 aactctattt gccgcgacat atcccgggca tcccagcttt ttaccgtatc gctgatgtgg
     2641 cgcgtcagga atgcggaaaa ctcaggcgcg acgcggtgcg cggcttgttc caggtgacca
     2701 tttaacgacg cccgcaaggc atcatcggca attaacgttt cgccaaacca ttgacccgcc
     2761 cgcgcgatac gttctttcac acgagaatct tcactgttga tatccgcttt cagccattcc
     2821 cgtaaatccc cccacaattc actgagatag cggttaaaag cttcatcttc tttcagataa
     2881 ctttttacgg catcggctcg cgctgccatt tcaggatcgt ttttcagctt gtcgatcagg
     2941 gcaaaggtgg cgcgatcaaa tgcatggcgg atctgatgcg cacgatcgcg gctgatatca
     3001 tcaagtaaag aattcaccgc gtcagaaacc aactccgcgc tatgttcgcc caaccattcg
     3061 gtggggagaa ttttggcttt cagtggatgc tcgctctcca gccagcgaac aatttgctgg
     3121 gcgataaact tgcgcgattt atcgcgctgg agaagggcga tcaactgtgc gatcagcgta
     3181 tccagcagca cctgatgacg atcgtttttg gtcatactct ccagcatcaa cgcactggtg
     3241 ccggaaagat cgaccttatc aatcgcccga tggaccgcgc gcttaagcag gcgctgaata
     3301 cgcgcatcat cggtaagttc gagaaaaccg ctcatgatct gcaacagatg ctgaccaacg
     3361 cggcgggcgt tttctggctg gctaaaccag ttgccaatca gcaacgccgg ttcgtggcgt
     3421 cgaatcaatg ccaccaggga ttgagtatca agaaattttt cctgcacgaa ctggccgaga
     3481 ttttcgccaa tccggtcttt attacgcggg ataatcgccg tatggcgaga aatgatcgga
     3541 atcggcacgc ggcgaaacag cgccaccacc gcaaaccagt ccgccagcgc gccgaccatc
     3601 gccgcttcag caatcgcctt cacgccgctc acccaaaaat tgggcggcaa aaacagcgta
     3661 acgacaaagg tagcagcggc gataagcagt aaagagagcg ccaacatttt ggcgcgtctg
     3721 agttcaatga gtttattcat aacgttaaga atagagcctg acggcaggat cgcgcaaaaa
     3781 tcactgctcc tctgccagct cggcagcctg atgttggcgc acgcgattaa gcaatcccgc
     3841 cagggtgaaa acgacgataa cgcccgccac gacggctaac agatgaaacg gcaagcgacc
     3901 gccgttaaac cacagccagc ggccgatttc gaccgagaca gacatcacca tcaggatcac
     3961 catattcagc gatgcggaga cggtcccttt cggtaagtta ttggaaaaca gcgtaaagcg
     4021 gaataaggtc gggaaaatca aaccaatccc gaaagcatac agactggtgc ccagcaccga
     4081 ccaaagccag acgtgcggcg acagcagatt gccgataatc aacagcgcga ggccaaccag
     4141 ttgaatgggt acggcacgcc agataaagcg cggttcggtc ggatctttaa cgaaacgcgc
     4201 cacgatggca ttcgcgacaa tcaccgcgcc gaataccgga acctgtgtcc aggcgaactg
     4261 cgaggtcgtt aagccgcctg cgtcaatcag gatcaccggc gagacagcca cccaactcat
     4321 catcgggata tagcttaaag agatggttgc tgcaccaaag aggaacagcc gattgcaaaa
     4381 gacattacga aaatcgcgca agacgctttt ggcgctaaat ggaaccgcgc cgcgcttcac
     4441 cgtctctggc atcgccagca gtaagccaac aaatgagata aaacccataa ccgcaatgat
     4501 ggcaaaaagg actttccagt gcacaaagtg catcagagct gcgccggaaa gcgggccgat
     4561 aatcggcgca atcagtacga tggaggtgat aatcgccatc aacttgatcc cttttgtctg
     4621 gccgaacgcc tcctgcaccg tgacataacc aacggtagca ataaaacaga tactggtgcc
     4681 ctgaattgca cgcgcgataa gaaactgtgt catagatgtt gtgaacattg tcgcggcgca
     4741 ggcaagggta aatattagcg ccccggtaat cagcaccggc ctgcggccaa tcctgtcgga
     4801 aagtggcccc agcagccact gtaacgccat accgccagcg agatagaggc tgaccgcagc
     4861 aggggccaga ctgacatcgg cattaaaatc acggaccaca ttaatgatcc ccggctggat
     4921 cagatccgtc gaaagatagg cggcaaagtc atacaaaatc aacgccatcg ggaaaaacag
     4981 cgtagcggca tggcgggcaa aaaaacgtgg cataacaaac aactccttgt ccggagaacc
     5041 ccaacgggga aaacgaaatt caataccgtc ggtaatgacg aaaggcaggc gagaaggaca
     5101 aatgtactga atttcatgta atcaaccaca ctgcctgtga acacacagac agagaaatac
     5161 cttctgactt cgggtcagaa tgtataacga ttacaaatag tcatcagcac attacacctc
     5221 ttgaattgat gggttgcata gaaaatatac accttaagtg taattaaaat ttgcggttaa
     5281 tcaaaagagc gcggtggaaa ggggaatatc tgccgggtac aggaaatgta aaacgcgagc
     5341 ctgtttccgg aaaaatgtat cggttgcaga cgaacaaaaa tagcctggaa agcgcctcgg
     5401 ggaacgggaa attgccgggt gagaatggtt ttgttagtcg ctaaagtcgg gccatcttat
     5461 ctacaggtga cggatcgcca tgacaaactt cacgaccagc acgccgcatg acgcattatt
     5521 taagtccttt ctcactcacc ctgacaccgc gcgggatttt atggagatcc acttacccaa
     5581 agatttacgt gaactgtgcg atctcgacag cttaaaactg gaatccgcca gctttgtcga
     5641 tgataaattg cgggcgctac actccgatat tctgtggtcg gtaaagaccc gtgaaggcga
     5701 tggctatatt tatgtggtga ttgaacatca gagccgcgag gacattcata tggcctttcg
     5761 cctgatacgc tattccatgg cggtgatgca gcgccatatc gagcatgata aacgccggca
     5821 gctaccgctg gtcatcccga tgctgtttta tcacggtagc cgtagtcctt acccctggtc
     5881 cctgtgctgg ctggacgaat ttgccgaccc gactaccgca cggaaacttt ataatgccgc
     5941 gttcccgctg gtggatatca ctgtcgtgcc agacgacgag attgtgcagc accgcagagt
     6001 cgccctgttg gagttgatcc aaaagcatat tcgccagcgc gatctgatgg ggcttattga
     6061 tcaactggca gtattactgg ttacagggtg cgctaatgac agccagataa ccgcgctgtt
     6121 aaattacatt ttactgactg gcgatgaagc gcgttttaat gagtttatca gtgaacttac
     6181 ccgtcgaatg ccacaacaca gggagcgaat aatgacgatt gcagagcgaa ttcataatga
     6241 tggatggctg ttagggatgg aaaaggggaa agaagaaggg gaacaacgcc tccttagatt
     6301 gcttttgcag aacggtgcgg atcctgaatg gatacaacga tatactggac tttcggcaga
     6361 gcaaatgcag gcattagagc aacccttgcc tgaaagcaag cgcgatccat ggatcgagta
     6421 ctaatcagag actgatgaca aacgcaaagc tgcctgatgt gctacgctta tcagacctac
     6481 attacctctg caatctattg aatttgcgcg atttgtaagc cggataaggc gttcacgccg
     6541 catccggtat gaacaaagca cactttgcct aagccatgct ggaacgtggc gatgcccctg
     6601 caaagataaa acgagtcaca ggcgattcgt gagaggaaat caggggcctc gccactaatt
     6661 cgaattcatt ccattgcctg atacacggcc tcgccaattt gcttcagtgc ttcgcgatag
     6721 gtttcgctga gcggcaaagc gcagttgatg cgcagacaat tacggtattt gccggaagcc
     6781 gagaaaatcg aacctgccgc cacctggatt ttcatgcggt acagctgccg cgcgacgcag
     6841 accatatcga cctgttcagg caattctatc cacagtaaaa atccgccttt cgggcgcgta
     6901 atacagattt cgcagggaaa atattcccgt atccagcagg tataaagcgc caaattgcgc
     6961 tgatagatct gccgcatccg ccggatatgg cgatgatagt gaccttccag cacaaacgtc
     7021 gccgccgcca tttgcgtgga cggtacatta aagctgctga tggcatattt catatgcagc
     7081 agtttatcgt gataacgtcc cggtgcgacc caacccacgc gcaggcctgg agcaatactt
     7141 ttactgaacg agctgcacaa cagcactcgc ccgtcgatat cccatgaatg aatggtccgc
     7201 gggcgcggat actccgttgc cagttcgccg tagacatcat cttcaaaaat cacaatatca
     7261 tgacgctgag cgagagagag aacggcccgt ttgcgcgcgt ccggcataat aaatcccagc
     7321 ggattattac agtttggcac cagaatgatg cctttaatcg gccactgttc cagcgccagt
     7381 tccagcgctt caacgctgat gcctgtttct ggatcggttg ggatttcaat cactttcacg
     7441 cccatgccgc gcagcatctg cattgaaccg taataacagg gggattcgac cgcgacaata
     7501 tcgcccggtt tacacaccgc cattaacgcc atcgacatcg agttatggca gccgctggtg
     7561 atgatgatgt catcggcggt gaccaccgag ccgctgtcga gcatcaggcg ggcaatttgc
     7621 tctcgcaaca ctcgctgacc ggctaacaag tcataaccga gaacggtttg cagattatgc
     7681 tgtaccaccc ggcttagctc ccgccagagc ggtttcaggc tgggcgtttc gacatccggc
     7741 gtgcttttgc ttaacggaac aatggaactg tcgctatgcg cctccagcat atccagcacc
     7801 tgatcccact gggtaatttc caccgggcgt tgcaccggac gcgtcatcgg cggcactggc
     7861 ggctgggctt tacgttgtgc gacaaaataa cccgaacgcg gctgcggagt gatgagcttc
     7921 atcgtttcca gcgtctgata cgcctgctgc acggtgctga tgctgacgcc gtgctcctga
     7981 cttaagctgc gcaccgacgg caatttctcc ccgtgacgat acagcccttg ctcaatccgc
     8041 tcggcaagca gagttgccag atgttgataa cgcgtcatgc tgtattcctt atttggacca
     8101 tacagagaga aaaaccggta cagatgaggc taaaaaaccg gatgcagatg ctgttttaac
     8161 ctgtctgtat gttaaataaa actaatattt gcatctgtat tgtgaactgc cagaaggcag
     8221 atgatgaagc ctctgaaaga tgaggaggca gcagatggaa tttcacgaaa acaaagccaa
     8281 aacgccgttt atcagacttg tgcaactctg gcaggcggtg aggcgttggc ggcggcaaat
     8341 gcagacccga cgagtgttac agcagatgag tgatgaacgg ttgagggata tcgggttacg
     8401 cagggaggat gtggagtgag ggggcaattc atcgcggata aaccggattt cgctataacc
     8461 ttcacaagga agtggttatt attgcgtcat tattgcttca cggaaggacg ccaggatgaa
     8521 agccacagag gctcgcctac tcgatttttt aaaacgttcg cagcagtttg ttattcccat
     8581 ttatcaacgc acttattcat ggaccgggca acaatgtcgg caactttggg acgacatcat
     8641 tcgtgcagga aagcgtgacg atatatcagc gcattttatc ggttcggttg tttatataga
     8701 gcagggattg tatcaggttt ctggtatttc tccgttgctg gtcattgatg gtcagcagcg
     8761 gctgacgacc gcaatgttgc tgattgaggc tttatcgcgc catcttggcg aagacgaagt
     8821 ttttgatggc ttttcagcaa tgaaattgcg taattattat ttgctcaatc cttatgagtc
     8881 cggcgagaaa ggatttaaat tactgctgac cgagactgat aaagacagtt tactggcgtt
     8941 aataaaacaa agaccaatgc cagaaaacta ttcccatcga ataatggaaa actttacttt
     9001 ctttgatgaa caaattacca aactcggtga tgacttgatc cccttatgtc gtgggttagc
     9061 aaagttatta attgtcgatg tggcgcttaa tcgtggtcag gataatccgc aactgatttt
     9121 tgaaagtatg aactccaccg gtaaggcgtt aagtcaggcc gatctggtgc gcaattttat
     9181 tctgatgggc ctcgaaccag agcatcaaac ccgattgtat gaagatcact ggcgtccaat
     9241 ggaagtcgcc tttggtcagc aaggttacag cgaatatttt gacagtttta tgcgtcatta
     9301 tctgacggta aaaacggggg agatccctcg gacagatgaa gtctatgagg catttaaact
     9361 ccatgcccgc agccagagtg ttgctgaaaa aggcgtagat cggctggttg aagatattca
     9421 tatctacgcg gagtattact gtgcaatggc attgggaaaa gaaagtgaca aattgcttgc
     9481 tacggctttt caggatttgc gcgagttaaa ggtcgatgtg gcgtatcctt tcttactggc
     9541 gctttatcat gactataaaa atggcgtttt gtctcacgaa gatttcctga gcataattcg
     9601 tttaattgaa tcttatgttt tccgccgtgc agtatgtgcg attccgacga attctcttaa
     9661 caagacgttt gctacttttt ataaggtcat taataaagga aaatatctgg aaagtattca
     9721 ggtacatttt ctgaatctgc cttcatatcg tcgtttcccc aacgatgatg aatttaaacg
     9781 ggaattaaaa gttcgcgatc tctataactt ccgcagtcgc agctactggt tacgacgact
     9841 ggaaaacgat aaacgcagag agcgcgtaga agagtttact attgagcata ttatgccgca
     9901 gaacgaaaat ttgtcggcta aatggcgtga agagctggga agtgactggc aacgcattca
     9961 taaagaatta ttgcatacgt tggggaatct cactttaacg cgctataact cccgctacag
    10021 tgacagacct ttcgcggaaa aacgcgatat tgaagacggc tttaaacata gcccgctcta
    10081 tttgaatatc ggtcttggac agtgcgaaaa atgggatgaa gccgccattc acgcccgagc
    10141 cgatcgtctg gccgatctcg cggttcaggt ctggcaagcg ccttctcttc ctgaagaggt
    10201 tttagctgtt tatcgggggc agccggaaaa caaaaccagt tacagcctga gtgattatcc
    10261 ttttcttgct gatggttcgc atagccgggt gttattcgat catcttagcg atgaagttat
    10321 gcgcctggac gcagggatca cgcaggaagt attgaagctc tatattgcgt ttaaagctga
    10381 aacgaatttt gttgatgttg tgccgcaaaa aagccgactg cgattgtcgc ttaatatgca
    10441 gtttcatgaa ctggtcgatc cgaaaggtat tgccaaagat gtgacaaatg ttgggcgctg
    10501 gggtaatggt gatgtggaaa ttggtttcag tgacctcgca caacttcctt acattatggg
    10561 attaattcgt caggcatttg aaaaacagat ggagagcgcg ttgatataac gcggcgaata
    10621 atacccggta gcactggtgc taccgggtca atgtatcagg caactttctg tttaccggca
    10681 atcaccccaa taaattcctg cacttgcttc tgtttacgcg ccgacagctt acacaccttg
    10741 cgtagtgact gcatcagttc gctctcttcg tccgcgggtg gttgggcggt gaggacaata
    10801 cagccttcca tcactttgac atctaccgcc gtgccggtag caaaaccggc ggcttccagc
    10861 cactgacctt tcagggtgat ggcgggaata cggctgtaat ccgggtagcg gctcgcataa
    10921 ctcacggtta attgacggtt atttgccggg gagacttctg cttcgaacgg ttgtgcaata
    10981 gaatgcgtgt cagtcatgat tactattctc tataaatagt gattgtgatt agcggtgcgg
    11041 gtgtgttggc gcacatccgc accgcgctaa atacctgtgt atatcatcag taaatatggg
    11101 gaaagtccag ctaaaaatta atgaaaataa gatgaaaatt agcgtgagat ataatt
//
LOCUS       DAPGRN010000136        10951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPGRN010000136 DAPGRN010000000
VERSION     DAPGRN010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.138"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1834)
                     /gene="selB"
                     /locus_tag="RHW33_004016"
     CDS             complement(<1..1834)
                     /gene="selB"
                     /locus_tag="RHW33_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV1647471.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLF"
     gene            complement(1831..3222)
                     /gene="selA"
                     /locus_tag="RHW33_004017"
     CDS             complement(1831..3222)
                     /gene="selA"
                     /locus_tag="RHW33_004017"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV1647472.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3320..3928)
                     /locus_tag="RHW33_004018"
     CDS             complement(3320..3928)
                     /locus_tag="RHW33_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV1647473.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3999..5135)
                     /locus_tag="RHW33_004019"
     CDS             complement(3999..5135)
                     /locus_tag="RHW33_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV1647474.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5138..5500)
                     /locus_tag="RHW33_004020"
     CDS             complement(5138..5500)
                     /locus_tag="RHW33_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV1647475.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6037..7950
                     /gene="mtlA"
                     /locus_tag="RHW33_004021"
     CDS             6037..7950
                     /gene="mtlA"
                     /locus_tag="RHW33_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV1647476.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGAERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8180..9328
                     /gene="mtlD"
                     /locus_tag="RHW33_004022"
     CDS             8180..9328
                     /gene="mtlD"
                     /locus_tag="RHW33_004022"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV1647477.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGNDVVDIIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDANAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9328..9915
                     /gene="mtlR"
                     /locus_tag="RHW33_004023"
     CDS             9328..9915
                     /gene="mtlR"
                     /locus_tag="RHW33_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV1647478.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9927..10136)
                     /locus_tag="RHW33_004024"
     CDS             complement(9927..10136)
                     /locus_tag="RHW33_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647479.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10421..10783
                     /locus_tag="RHW33_004025"
     CDS             10421..10783
                     /locus_tag="RHW33_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV1647480.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTAF"
BASE COUNT         2644 a         2957 c         2800 g         2550 t
ORIGIN      
        1 gaaataataa tgcgtcgcgc aataaatgat catttccacg acgacgcgta aagccaatgc
       61 ggtcaaaata ttccagaatc tgaattgcca gctttcggcc tacgcctaag cgatcgcgga
      121 aatccgccgc gcaggttgaa ccacactcct gatcgagatc gcggatcata ttggcaaact
      181 cgacaatccg atcgttacgg taataacgat ctttgacgat cgcggtaatt atcccctgtt
      241 gggccgcctg gcgtagcgtc agacgcattg cctgctcgtc ggttcccgtc tcttttgcca
      301 ggtcacgcac ccaccacggc tcgtcaccaa acagcggctc tgctttttgc cagatggcct
      361 gctgctcttc gctgaagccc gctttgtgat ccggcagatg cagccaaccg tgatgactga
      421 ggatgtcgcc gctttcgcgc atcttttcaa tcagcaacag taccagcgct tcatcttcca
      481 ttggcaacgc catacgtcgc agacgttcgc gcccaggacc aggttcatca cgatgttgct
      541 catgataagt cgctaatgtg tcgagaattt tccgctgcca gcgggcggca accggcgcat
      601 tcaacaagct ataaccagcc tgaatatagc caggctgttg cagcagttcg cgcatccctt
      661 cgccgttgag ctggcgcgcc caggcgaaat ccgcaaggtt aaccgctccg cgttccagat
      721 gaacagataa cgcatcggca tcgctctgcg cccgtgcaag agacgccagc cattgcagat
      781 attccggctt acgtttaccg cgacgcggcg ggttaagcat caccacacgc gcccccgcca
      841 gcgtattgcg ggccgagata tcgcgcaaca ctaagcgatc gttatccgcc agccacagcg
      901 gcgtgtcgaa caccagctcg gcaaggttat cttccagcag cgaaacgcgt cccgtaacgt
      961 ggctggcggc gtggtgaata tgcagcggct gccactgggt cagcggcgta tgggtttgaa
     1021 gctcgacaat cacccgcgtg aacggctctg gcggcacatc ggcaagcagc cagtcgccac
     1081 ggttaatctg ctctttttcc gcatcacccg cgatgttaag cgcgatacgc tgcccggcat
     1141 gggcggtttc tgttggctgg ttttgtgcat gcagcgcacg tacacgcatc ggtttattta
     1201 caccagttag ccatagtgaa tcgcctacct tcacttcccc gcttaacgcc gtaccggtga
     1261 cgaccagccc ggcacctttt acggtaaatg cgcggtcgat cgcgaggcgg aaactatgtt
     1321 ggctggcgtg ttcacgttct ggtaactgaa gcagatgctc gcgcagggca tcaattcccc
     1381 gaccttcggt tgctgcggtg ataaacagtt ttgcctcagc aaaaccgtat tcccgcagca
     1441 cctcctttac ctggcgttca acctcatcaa cacgcgcttc gtccacgcga tcggctttgg
     1501 tcagcgccac tgtcagcatc gggttaccgg tcagctgcaa aatcgccaga tgttcacggg
     1561 tctgtgccat cacgccgtca tcgcacgcca ccaccaacag cgcgtgatcg ataccaccaa
     1621 cgcccgccag catgttggaa agaaactttt catgaccggg tacgtcgata aaaccaggca
     1681 cgcggccatc cggctgcggc cagtaggcat agccgagatc gatagtcatg ccgcgctttt
     1741 tttcttccgg cagacgatcg gcatttacgc cagtaatcgc ctgcaataag gttgttttgc
     1801 cgtggtcaac gtgtccggca gtcgcaataa tcatttcaac aacatctcca aaaaccgttg
     1861 ctcatcttca aggcagcgta aatccagcca caatcgtccg tcataaatac gaccaatcac
     1921 cggcactggc aattcacgcc agcgggcggc taatgactca aggtggctac cgcgtccatc
     1981 atggggtgta aacgttaatg ccgcgctcgg caggcgatca accggcagcg aaccactgcc
     2041 aatctgcgaa agacatggca taacctgtac cgcaaactcc gcgccgtaat gtgcggcaag
     2101 gggagcctgt aaacgttgtg cctggatttg aatgacctct gcgctgcggg taagcaggcg
     2161 cagggtcggt aatttttcac tcagagcttc agggtgtaaa taaagacgca acgtggcttc
     2221 caacgccgcg agggtcattt tatccgcgcg taatgcacgc ttcagcgggt ggctttgcag
     2281 gcgggcgatc atctcttttt taccaacaat aattcctgcc tgcggcccgc ctaacaactt
     2341 gtcgccggag aagctcacca gactgacgcc cgccgcaatc aactcctgcg gcattggctc
     2401 tttcggcaaa ccgtactggc taagatcgac cagcgagcca ctgcctaaat cagtcactac
     2461 gggaacatcc agctctttgc cgagcgccac cagttcagct tcgtctatcg ctttggtgaa
     2521 cccctgaatg ctgtagttac tggtatgtac tttcatcaac agtgcggtat tttcattcac
     2581 cgcctgacga taatcattcg cgtgcgtgcg gttggtggtc cctacttcgt gtagggtgca
     2641 gcctgcctga cgcataacat cgggaatacg aaacgcgccg ccaatctcca ccagttcgcc
     2701 gcgagatacc actacctctt tcccgctggc agtggccgcc aacatcaata acaccgccgc
     2761 cgcattgtta ttaacgatac aggcatcttc cgcccccgta atacggcaca gcagctgcgc
     2821 cagcgcccga tcgcgatgtc cgcgtccggc gtcgtccaaa tcgtactcga gggtcactgg
     2881 cgaacgcata gcctgcgcaa cggcttccac cgcagcttcc gcctgtaaag ctcgcccaag
     2941 attggtatgc agcacggttc ccgtcaggtt tatcaccgga cgcagcgcgc tctgcgcctc
     3001 tttcgtcaac cgggcatcga cttcttgcgc ccagttttca caccacgcag gcagcgtctg
     3061 gctgccacga atcacttctc gcgcttcgtc gagcatctga cgcaacaatt ccaccacgcg
     3121 ggtgtgacca taagtatcac gcaaagaaag gaaggagcta tcgcgcaata agcgatcaat
     3181 agccggaagt tgactataga gagaacgcgt ttcggttgtc ataggaaacc tggctgatca
     3241 aggccctctc acacggagaa gggcgtttaa cataaccacg gattgtaacg tgagatgggt
     3301 caggaggaca tatcgcgcat caagcctttg gcggttcggt gcgagcaaaa ctttcgcggc
     3361 taaacaggtt ttcgaccagt ttgactaagt gtgggcggtc gacacaccag cctggcgcaa
     3421 cgcggcggaa attgagatac ccgacggcac aggcaatggc gatagtcgcc agattgaccg
     3481 tatcggtttt gagtgtgcca tcgaccagat atccttccag cacatccaga ctgcggttga
     3541 ttttctcccg ctggcgtaac aattcatctt cagactgctg cgccgctgga cgcgcctgtt
     3601 cacgcaccga taccagccca gcatccataa tgccatccgc cagtgcctca attttgcgca
     3661 cccgcaacga ctccagcgga tcgcgcggca acatcgccgg agcgacattc attaattcaa
     3721 tatattcagc gatgatcggc gaatcaaacc agcattcgcc ctcttcggtc accagcaccg
     3781 gcacttttcc taacgggtta aattgcgcca cgccgttgtc cgcgttatag ggcagttcat
     3841 taataaattc gaaagttatg cccttttcta acaacagaat agaaagtttg cgtacaaacg
     3901 ggctggtgta gctaccgacg agtttcatac cgagtccttt gtgcgaggaa aaatatcagt
     3961 atggctcagg ctcacgcaaa acacaggtga tataagtctc aatgatccaa ataaagataa
     4021 tgcatccagc tggtcattct tagcagcact tttcgcatca ccgaaacatg gctgaaatgg
     4081 tcggagtaaa ctgccacctg ggcgtaaatg ccatcgggta aggcatcgat atcatcgtta
     4141 gggtccagtt caatggttcc cagcacaccg tccgtgccgg gcacgaccgt taatgattgc
     4201 aataccccct gcgcctgata agaaccgcct ggcacgacag gtaaaatact ggtcagtttg
     4261 ccgtggaaca cctgcccagg tagcgcgtta aacaccactt ccgcatcatc gccaggtttc
     4321 agacgtaaca gcgagttttg ccgaaattgg gcgacaattt gccgtttttg ctcggggatg
     4381 aaaaccatca ccggacgcaa cggcaaggca gctgcgtatg tgcctgggcg gatcagtacc
     4441 tgagtgacgt agccattgct tggtgcgcga atgacagtct gctcaagatt atattttgct
     4501 tcagtaagtt gcgctcttaa gctcacaatc tgcgattgct cgccgttaac catactgtcg
     4561 agctggctct ggatctgcgc ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg
     4621 aggaaatttt gccgcgcatc gtcgatgtca cgttccgaga acggattcac cgccgcctgg
     4681 ctgcctttca ggtaacgttg ataattttta aacagacggt cgcgctccgc tgaaacctgg
     4741 gtggtgttgg cctgcgcttc agtgagctgg gcgcgcagcg tttttatatt atgcgtcgcc
     4801 gtcatcaggt cagcctgaag tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta
     4861 aaaagcacct cgcccttttg aataagctga ttattcttgt cagtgacttc agtaacaatt
     4921 cccgtcacct gtggcgtgat agggatcgct atcactgcct tttgcgcggt aaaagtgtaa
     4981 gggtggttgt agttcatcaa caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc
     5041 gccgtcgcca gcgtccactg attcaccgga atacgaaaga ttttaaagac cgcccacgcc
     5101 agcgccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
     5161 agtcagtctt tttctcggcg gcgagttgat gctccagccg ggaaattcgg tcagaaagtg
     5221 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc ataccccagc
     5281 cacgctccgg ttgatagagc gtcgcccaga tccacagaaa cggccagata acatgcagag
     5341 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
     5401 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
     5461 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atttgcctcg gattcacgtt
     5521 tatcagtgtt gttttttggc tggcagccag aagggagtca ggctgatatt ttgacaataa
     5581 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
     5641 tagttacata acgattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
     5701 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
     5761 tgtgacatta ctcacattta aatgccattt ttagcgaaaa ccgccgcctt gttgcttttt
     5821 tacacaagca ttttgtgatg aacgtcacgt caattacctc tctaccccct atatttatgt
     5881 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
     5941 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
     6001 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
     6061 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     6121 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     6181 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     6241 ggtaagctgg taggcgccga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     6301 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     6361 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     6421 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgccattct ggcatttctc
     6481 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     6541 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     6601 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     6661 ctgggtaaat ccatattctt cctgattgaa gctaacccag gtccgggtat gggcgtgctg
     6721 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatt
     6781 atccacttcc tgggtggtat ccacgaaatt tacttcccgt atgtgttgat gaatccgcgt
     6841 ctgatcctgg ccgttatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     6901 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     6961 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctcctttgtt
     7021 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     7081 actcgtcgta tgcaggacat gaaagctgag tctaaaggtg catctccgct gtctgcgggc
     7141 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     7201 ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtcg
     7261 cagatttcgg taactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     7321 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcaca gcatatttcg
     7381 ctgaccaact tcctcgacag cggcctgtac accagcctga ccgaacgtct ggttgctgcc
     7441 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     7501 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagctgcc
     7561 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggtgg ttacgttgag
     7621 ccggagtacg ttcaggcgat gttggatcgt gaaaaactga ccccgactta tctgggtgag
     7681 tctatcgcag tgccgcacgg tacggttgaa gcgaaagatc gcgtactgaa aactggcgtc
     7741 gtgttctgcc agtacccgga aggcgtgcgc ttcggcgaag aagaagatga cattgcccgt
     7801 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     7861 aacgcactgg atgatgagtc tgtcatcgag cgtctggcac acaccaccag tgtggatgaa
     7921 gtgctggaac tgctggcagg tcgtaagtaa cccaatccca ccctctccac atggagacgg
     7981 tggggctgat tgcctgatgc gcttcgctta tcaggcctac aaaacaaagc gcagtttgtt
     8041 gaatttgcac gttcttgtag gccggataag gcgtttacgc cgcatccggc gctgcccctc
     8101 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     8161 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     8221 ctttatcggt aaactgctgg cagacgcggg tatacaactg acgtttgccg atgtcaatca
     8281 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg taggtgaaac
     8341 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggta atgatgtcgt
     8401 tgatatcatt gctcaggttg atttagtcac taccgccgtt ggcccggtag tgctggaacg
     8461 tattgctccg gctatcgcca aagggctggt gaaacgtaaa gaacaaggta atgaatcccc
     8521 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     8581 gatgaacgcc ctgccagaag acgccaacgc gtgggtagaa gaacacgttg gctttgtcga
     8641 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     8701 gacggtagaa accttcagcg aatggattgt cgataaaact cagttcaaag gcgcactgcc
     8761 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     8821 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     8881 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     8941 aagtggcgcg gtactgatca agcgttacgg ctttgacgct gacaagcatg cggcgtacat
     9001 ccagaaaatc ctcggccgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     9061 ccgtcagccg ctgcgtaaac tgagcgcagg cgaccgtctg atcaagccac tgctcggtac
     9121 gctggaatat ggtctgccac ataaaaacct gattgaaggt attgccgctg caatgcactt
     9181 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     9241 gcaggcggcg ctggcacaga tttccgggct tgatgccaac agcgaggttg tatctgaggc
     9301 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     9361 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     9421 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     9481 ctcctgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     9541 gtgaagtatg ctgtagaacc gttactcgac ggcgatggtc cgctgggcga tctttctgtg
     9601 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     9661 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     9721 gacgaaatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     9781 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     9841 gtgcgatcaa caatggtcct ttcactgaca gagctgattt ccaaaatcag cttaaaaaaa
     9901 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     9961 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
    10021 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
    10081 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
    10141 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
    10201 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccact tctgtttatt
    10261 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatattaa
    10321 tgattacatt gcggaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
    10381 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
    10441 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
    10501 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
    10561 agaaattgcc cgcctgcgtg aagttcacag ccagaaactg agtaaagaag cacagaagct
    10621 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaaact
    10681 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgcgaaat
    10741 gggcctggaa gagatgactg gattctcaaa gactgcgttt taagcccctc agacgattgg
    10801 tacgatcgta tcaacccgca tctggcttgc gttagctggt caatgcgggg actcccctgt
    10861 ctgctcgcct aattactgaa caatccactc cctctacatc aatttaccgc tggttgatat
    10921 tctgaaaatg tattttcacc gaaatagcaa c
//
LOCUS       DAPGRN010000137        10868 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPGRN010000137 DAPGRN010000000
VERSION     DAPGRN010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.139"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1578)
                     /locus_tag="RHW33_004026"
     CDS             complement(<1..1578)
                     /locus_tag="RHW33_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV1647481.1"
                     /translation="MNDVTVVTSVTYPSSESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVARTYTDSYQLGDGELLVCTVNIPASVSA
                     ITQEMIDTSERINRSIGIDISDSVTSTRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSATNSDSETMAATPKAVKSIKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNYSTT
                     INNALALKAPLASPALTGVPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTL
                     NELAAALGNDPNFATTMTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDL
                     TSVGRDILAKTSTLAVIQ"
     gene            complement(1590..2024)
                     /locus_tag="RHW33_004027"
     CDS             complement(1590..2024)
                     /locus_tag="RHW33_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647482.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFELAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            complement(2103..2939)
                     /locus_tag="RHW33_004028"
     CDS             complement(2103..2939)
                     /locus_tag="RHW33_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647483.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            complement(2939..4372)
                     /locus_tag="RHW33_004029"
     CDS             complement(2939..4372)
                     /locus_tag="RHW33_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HDV1647484.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVQNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRGSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            complement(4369..4725)
                     /locus_tag="RHW33_004030"
     CDS             complement(4369..4725)
                     /locus_tag="RHW33_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647485.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            complement(4725..8147)
                     /locus_tag="RHW33_004031"
     CDS             complement(4725..8147)
                     /locus_tag="RHW33_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1647486.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGGRVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVEAMKRLNLGDVSRVVQEDVALQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKARRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQEQTSEIKEGNDKILSALDQIAKNTGKKKGSLLSKLFSLLGKGAGGV
                     ASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYDAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     MGGLVSGISSAIGEALKSLGFEDIGNMLQSFSTESIAQAIDSGITNLETYISNLGDTI
                     STKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ELKAPVNSHQETSNSNTESDAKQSNIATRVINAALDTAKDSNKTVKQTANQIINANAV
                     ETGNSALQKIDKAIGQNSSSSSSLNTTGTRNDIQKAADTYNNGNLDVKVGSLGAEGKA
                     NLDKLAPYFAELENKYGLPEGTLYAIAATESGGDPNAKSTLTRSPNGKLSGGALGMFQ
                     FTSVAREETGLSREDSFNPEKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKW
                     KKGTGDLSKENREYAIKVNTHRARYLGGEIYTPGAGAQGGAQYGVRGPLPDNAVIDQS
                     TGLAFTPGDSPFEKGGLVDKIGNDVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGT
                     ATAAGNVYVDTPMPVEEARPVANNSSYFDQLGAQMGIDGLFDKLRNSPGMRKNNAPEP
                     ASTSQVTTAANDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQ
                     MTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            complement(8229..9110)
                     /locus_tag="RHW33_004032"
     CDS             complement(8229..9110)
                     /locus_tag="RHW33_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HDV1647487.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKHDPLTWTAQDRITALWWIFTGSRETPAETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPSEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDKKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            complement(9125..9736)
                     /locus_tag="RHW33_004033"
     CDS             complement(9125..9736)
                     /locus_tag="RHW33_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1647488.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            complement(9747..10313)
                     /locus_tag="RHW33_004034"
     CDS             complement(9747..10313)
                     /locus_tag="RHW33_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647489.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            complement(10394..>10868)
                     /locus_tag="RHW33_004035"
     CDS             complement(10394..>10868)
                     /locus_tag="RHW33_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV1647490.1"
                     /translation="LQSQIAAIAQAENEGRAKEQQAEDARKELIRQQAQAERIRREKA
                     ASAAAAREKQRVAAENERRAKREAELANDKKRDQAYEDELRKLQLESMKLELQAKAAR
                     VQRENDFIEQELKERAAKTDVIQSEADANRNISTGSKDLLQSEGKAREKKASSWW"
BASE COUNT         2619 a         2766 c         2383 g         3100 t
ORIGIN      
        1 ttggataacg gccagtgtgc ttgttttagc cagaatatcg cgaccgactg acgttaaatc
       61 agtctgagct accgtatcct taccagtgaa ataaggcagt ttgtttgcac cagtcgcaag
      121 ggcagcgaga gcggttaaag ttgcatccag aggctgtttg cctgccagcg catttgtcat
      181 tgttgtcgca aagttcgggt cattgccaag tgctgcggca agctcattca gggtatcaag
      241 agcttctggt gatgaaccaa ccaatgcgga tatggcagct cttacataag cggtcgtagc
      301 aatctgcgtg ttattcgtac cctgtgcagc ggtaggcgca gtagggacac ccgttaatgc
      361 agggcttgcc aaaggcgctt tgagagccaa ggcattgttg atagttgtgc tgtaattcgg
      421 gtcgttattg atcgcagcag ctatttcttt aagcgtatcc agtgtgccag gcgcaccgtt
      481 gataagtgcc gttatagctg ccttaacaaa ggctgtattt gcgatctgcg tgttattcgt
      541 accctgtgca gcggtaggcg cagtaggtat tcccgttaat gcaggacttg ctaaaggagc
      601 tttaagagcc agagcattgt tgatagttgt gctgaaattc gggtcgttat tgatcgcagc
      661 agctatttct ttaagcgtat ccagtgtgcc aggtgcaccg ttgataagtg ccgttatagc
      721 tgccttaaca aaggctgtat ttgcgatctg cgtgctattt gtaccttgcg ctgccgtcgg
      781 cgcggttggc gttcctgtca gactcgggct ttctattggc gctttggtat cagccagatc
      841 ttttatagac ttaacagctt taggggtagc cgccattgtt tcgctgtcgc tgttagttgc
      901 gctactgagc tgaactaatc ccttttgcgt tgtgcttgca tcctgtgccg tatacttgct
      961 tttcgccaga tcgtaggctt ttttaactgc cagcgaactt gcagcaacat cacttctggt
     1021 actggttaca gagtctgaaa tatcaatgcc gatcgagcgg ttgatacgct cggatgtatc
     1081 aatcatctcc tgggtaatgg cagatacact agcagggata ttcaccgtac aaacaagcag
     1141 ctccccatct cctaactgat atgaatcggt ataggttctg gcaacaaatt cagccgcatg
     1201 aatatgtgac gcggtattca cctggtaggt atcttctcca aggaggtatc ttcccttcag
     1261 cacaattgca tatttcttgc ctgcactaag tgcaagagaa atatccttac gttgctgaat
     1321 agttacctgg taaaattcac caatatccac cgacgccgcg cctgcggttt tatcaccatc
     1381 cactgaggta attaacaggt tcatcccacc gccaggctta ggtaagaaac cggcataaaa
     1441 tcccgggtca acaatccccc tgaattttcg gtttagcgcg gctgacagat atggttcgtg
     1501 gtattgcaca tcggccacca gagccaacga ctcggatgat gggtaagtaa ccgatgtaac
     1561 aactgtaacg tcattcatca agcatatcct tatgctgtag tcgtgtttat ggccataact
     1621 gcggtatatg ttttgcccac atacagcgag tcttcctgga cacaaataat ggcgattggc
     1681 ttgttctcgt tatccagaac aaccagagtg ttgaatgggt agtttttccc ttcctgcaac
     1741 tggctttgat caaggtccat tcggacagta attatcccgc ctgagtaggt tgggacgagg
     1801 ttgatggtgc aaaattgact ggtcagttct gccagttcga aagcctttgg cagttctcca
     1861 atctcataag tgccatctcc tttcttagta accagcgaac tggtaccgaa aacggccttg
     1921 ctgattaaaa atcgagagcc tttgttaatg gacgattcag cgcgccgctg atagtaatag
     1981 tccaacaact gactcttata gaggtttgtt gagacgtcag acatgatttt ccctaatcaa
     2041 tgttgtgaag cctcattgta agataagtaa cttgtcaccc cgccctgcgg acggggtgat
     2101 tatcaggcat cactatccag cagcaaatca tctgcgcggg tgcgatcaaa cgtaggcgtt
     2161 gctttcacaa tagtgccgcc cggcgttgca gtgatcggag cgctaatcga cgtaactcca
     2221 gtaagcgaag ttgtatccga agtttcaaac cagcagaatg ctttttcggt atcagaaatc
     2281 tcgttcaaag tgatcatgtc ggcctgttca tttacaacaa ccgacaaata gagcgtaagc
     2341 ccatcaaaca ctatatgcag tggcagtaga ggctttacga actgattaaa ctttctgaga
     2401 atttcttctg taattgcgga ctgatctatc gtgccagtaa tccccattgt ccgggccagg
     2461 tcgtttatgg gaatactgat catccctctg gaagtcagaa acatctcgcc gaatgtgccg
     2521 ccggtagtct ccagtgtgct ttctggtatt agaaccgtgc catagggatg acgctcaagg
     2581 tccaccggtg catatatcgg atcccataaa acagaaatac cgttaaattc gcggtaaatt
     2641 gtctggttta tagggcgttc agtcccctta aagtgaatct catcaagacg ctgttgtaac
     2701 aacatcggaa cggaagatga gttcgacgtt ctgatagtaa agaactggcc aagttcattt
     2761 gtcctggtct ccagatcctc cttgctcatg gaaaaaatag acttccggtt ggtaattcgc
     2821 tccaaccatg ggtcaacaaa ggtatccatc attgactgaa ccaaatcagc caatgattta
     2881 tagagcaatg acttttgctt agctgatgta agccggttat taaaccagga acgctgcatc
     2941 actcctcctc atacgaaata ttaaaggtgg agttttctgt atccagataa acgaaatcgt
     3001 aaaagccgtt ggactcattc cactcgacaa attccagata aaagtcgcgg aaataaccca
     3061 gcgtttcgat aaatgcccaa acgtcttttt tcttgattag gatgtacttg ccgacacggt
     3121 tcggatcaaa gaaagttgag ccacgcccaa attttgtttc cagtgccgac ttcagctcat
     3181 cagtcacgtt ctcaatggtc aggcttgccg atatccgccc ggtgatggtg atcttaaagg
     3241 gtagttttct gacctcttta tacgagaatt tcttgttcaa ctcattcggc accttcttaa
     3301 aggcagccag gatcatttct tcaagctctg actggctttt gtttggatgc catcctgaaa
     3361 taaatatctt attgatattc tgaacattat aagcaccatc taatttttct tgctgaccct
     3421 cgccccatgc ctttacccag gacagtcccg ggatgttacg cactagaaaa tacgtatagt
     3481 ccccgcccca tacgacctga tcatcatagg caaggtaata ttgtgcacgg ttacgtgtga
     3541 tctccgttgt ttcggcatcg gtacctgcgg ttataggtgt cgttgtctta actgaaatca
     3601 aattagctaa attagccgca gaatcgacag gcgtcaggtt ttggccagca accagggtta
     3661 tatcgccgtt ggtgcaccat accttaagcg taattgtcga gccttctggc ggtatttgcc
     3721 caattagccc atcgccgaat cgaaccccca actgctcgga tggtttataa aactcaacgt
     3781 agacctggct tttactaccg gctaaccgga acatagtgct ggaagaccac tgcgtggtct
     3841 taccatcggt cgtcacgaat acttccagct tatagcagac agcagtgaga gcctttgata
     3901 acacgacttc cagaaattct ttggctgccg taacggtata tgtcacctcc tggatttcca
     3961 actgtgccac ttctaccgta ccggtgccgt caaccaacct gcatacatcc atagtcatgt
     4021 aagggtactg gtcgtcagat attaaaggca tgtttttggg gattaccgct ggggcatctt
     4081 cacttgtggc ggtgatctca atcatccccg atgacggtgt tggcttggta ccaacgtaac
     4141 tattcgtttc tgccgctgcc aggatagagg aacgccgcgt cgcggtcgat ataaagcctt
     4201 cagccagcgc cgcatcggca tactgaaagc acctgtagac aatctgggta ataaacaatg
     4261 tcagcatcga gacaaattga gagccgacaa acttcgacca gaatgaatct ttctcgacaa
     4321 gctcttcaaa ctctgcacga atactgtctt tagtcggtgt tgttttactc atagcaccac
     4381 gtcctgtgtg atagttatat ccctgatacg aatggatatt ttcaacttat caaaagcatc
     4441 tccctcggct actgacaagc cagaaatcgg tatgtcaggt aaatctaccg tcagtttttg
     4501 caacagcatt gcctcaaccg caatttgaac atgcgacaag ttggtcggtt cgtgtttaaa
     4561 ctgcggtaaa acattgcccc atgacggatc tccgtatacc tcaccctgat aagtgtttag
     4621 ccactcatat aaacgagcgc cccaggcctc ctcctgggac tcatacgttt ttacgccgga
     4681 taactccagc gtcagcaaag gatcaatttc gttattgttg gccatcaatc aactctcgcg
     4741 tagtcattca tcaacggatc atcaattgac agtggcaccg tgcgcataac gcccggttga
     4801 ggcgtgctga cctttacgac agttccctgg cctttcgccg agtctttagt gtgctcttca
     4861 atcctggcaa gcaatgaggt catctgcgca aacagccgct tcgtttcacc atcaagtgaa
     4921 acggtattat cagccaactg cattgtcggc ttggcaccgg agccgccaag gtcactaata
     4981 acctgtccgt ctatctgcat acgaccggtt ggttgctgca aatcgttggc ggcagtcgtc
     5041 acctgggacg tggaggctgg ttcaggcgca ttatttttcc gcatccccgg cgagttgcgg
     5101 agtttatcga atagtccatc aatccccatt tgtgcgccga gctggtcaaa gtaacttgag
     5161 ttgttggcca ccggacgcgc ctcttcaact ggcatcgggg tatcaacata cacattgcca
     5221 gctgctgttg cggtcccctt ccctcgtgca cgttcttcga gcgttccctg aacgacttcc
     5281 cgacgcatac cccggccatt catgaatttg ttgaccagat cgttaacgcc aacatcattg
     5341 ccgattttgt ctaccagacc gcctttctca aacgggctat caccaggggt aaacgccaag
     5401 ccagtagact gatcgataac agcgttatca ggcagtggtc ccctcactcc atattgcgcc
     5461 ccaccctgtg ctcctgctcc aggtgtatag atttcaccac ctaaatagcg agcacgatga
     5521 gtattgacct tgatcgcgta ctcacggttt tctttcgata agtcacctgt gccttttttc
     5581 cacttattga tagtgccaaa cccagcgtta tatgcagtga tggcctcgtt taagtctcca
     5641 ttggcttgct tcagatactt gctcatgaga agagccgcag cttctgccga tttttccgga
     5701 ttaaaagaat cttcccggga taatccagtt tcctcacgag caacgctcgt aaactggaac
     5761 attccgagag cgccaccact tagctttcca tttggtgatc ttgtaagcgt agattttgcg
     5821 ttaggatcac caccagattc agttgcagcg atcgcgtaaa gagtaccttc aggaaggcca
     5881 tatttattct ctagttcagc aaaatacgga gccaacttat cgagatttgc cttaccttca
     5941 gcgccaagac ttccgacttt tacatccaag ttgccattat tgtaggtatc cgcagctttc
     6001 tgaatgtcat tcctggtgcc agtggtatta agcgaggatg atgacgagct attttgacca
     6061 atagctttat caattttctg caacgcgcta ttgcccgttt ctacggcatt tgcattgata
     6121 atctgattgg cagtttgttt aactgtttta ttgctatctt tcgctgtgtc cagtgccgca
     6181 tttatcacgc gggtagcaat attactctgt ttggcatcgg attcagtgtt agaattagac
     6241 gtctcctggt ggctattaac cggagctttt aactctggag tgatttcttt cgcattagcc
     6301 tcgccgatcg gattgggtat ttttgataca atcattgccg caggggtatt tttaacggca
     6361 tcaaccgctg catctaatgc tttaccgggt aaatttttaa ccccatccca aatattacca
     6421 gccgcctctt taatgtgttt ccctgggttc ttaatgaagt caattgcact atcaattgca
     6481 tcactgaaaa cctgtttcag gttatcgaca gtaaagaagt cttttatggc atccagcttt
     6541 tcaagcaact tattagatgt atcgctaaac catgctgaaa cagcatcacc aatctttgct
     6601 gtgtaatcat cgaacttggt agaaatggtg tcgccaaggt tagaaatata tgtttctaag
     6661 ttggtaatcc cactatcaat ggcttgggca atactttccg tcgaaaatga ttgcaacata
     6721 ttgccgatat cctcaaatcc aagtgatttg agagcctcac caatggcgct gctaatacca
     6781 gataccagtc cccccatatc aagaacatta gctaacgtat aagcggcttt ttgctggaat
     6841 gatggatctt gtcctgattt aagcccaaac gctcgacgtt gcgcttctgt atcattccaa
     6901 ccggttaccg catcataaat acctccagcc actgtgccga ctaggggaat tgcgcgtaac
     6961 gcccctttac caactgcctt taatccaagt ttacctgctg cccgggcagc caaatctccg
     7021 ccttcatggg cgagagtctt cttgccacca ccgcgtagca ttcctacaag tttctttgcc
     7081 cccagagcgc caaaagcgag tgctccagct tttttcagca tgccacgccc cattaacaac
     7141 gacgcgacgc caccggcccc cttccctaac aggctaaata gtttggacag caagctgccc
     7201 ttctttttcc cggtgttttt ggctatctga tcaagggcgc tgagaatctt gtcattgccc
     7261 tctttaattt cgctggtctg ctcctgaagt tcctgaaccg tccgtttttg ggtgttaacc
     7321 tgaacgacat cggcactatt ttgcgattta cgcctaaaaa aaccttttct acggctgtta
     7381 tcgtcattgc cacgaatcac atcggcaata gactttctgg caccattaag cgatccacca
     7441 acttcttttg atatcccgcc aagctccttc cctgctgccc acaatggacc agcaacggca
     7501 taacctaacg catcgacggc acgagtctct gaagggttac ctatgccttc agctactttt
     7561 gacagttttt ttaataaacc tgattcagca tttagacgct catcatcctc tttgcgcctg
     7621 gccttttcag cacgttcagc acgggcatct tccgctgcgg ccttactccc tgactttcca
     7681 ataaaacgac cacgcgcatc gcgttggttt tggctttttt gcgcaccgcc tttttgaccg
     7741 aacatttcgc gagcgtgttc ggctgcttcg gtccgttgcg cctttacatc ttctgttata
     7801 gccttcttgc gtcgtttttt accctttcgc gtagttgatt tggcctgcgg ttcctgtaga
     7861 gcaacatcct cctgaactac acgagaaacg tcccctaaat taagccgttt cattgcctca
     7921 acaatagggt ccactgatgg cgcattggcc acaaagtctg gccgggaatt ttcgattgtg
     7981 cgatttaatg ccgacacact gcgagagaca ggatcaacag ttgcaacgcg tccccctttc
     8041 aaatcttcaa cagcttcccg gatacctgca agctcttcca gctcttttgc gctggcggtt
     8101 tcaactgtcc ttataacatc gtcaatgttg gcgttttttc tttccatgat cttatcgcct
     8161 accgtttcgg tttaagtttt tcttccagct tctccaacag gaaaaacgca taggattcag
     8221 tgagcctttc agcgtcctga atcggtatac ccccatacaa aaccaggttg gacactaagg
     8281 tctgatagct tttcaatccc cacctgtgga atgaagtcgg tagcccgaaa gggcacccac
     8341 agacgggtat acgcaccctc tgtggactcc tttttatcct ggtttgggca tttgtgcggc
     8401 gggagacgaa gacgcatttc acctttatcg atgtagcacg gtaaaccatg ttcgagcttt
     8461 tcatgagcca gtcggatgtg tgccgccagt ttcataaatt cagtatcaat ggccatccgt
     8521 ttaatcgttt cataacgacg ctcagcctga tcttcacgag taccactaac atcgttataa
     8581 agctcacact gataagcgaa ttcccaaaaa cgcaaatcaa cgatcgcttc tttgaattcc
     8641 gcgtcgtctt cagatggcaa tgctgcacgg cgcatctcca gcatttccat tgcccaacca
     8701 tcaagcggca cgatacgcca ttgataaggc actccctcta cagacacctc aatatcgtca
     8761 atgaacggtt ccacttccag gacctggata tcttcagcca gagcattcat atcgcaatcg
     8821 taataatgct ctttaccgca atgtttacag gtgtaggtga atgtctcggc cggtgtttca
     8881 cgggagccgg taaatatcca ccataacgcg gtaatccggt cctgcgccgt ccatgtcagg
     8941 ggatcatgtt tcgcgggttc agccagcaag gcttttaaat acgccgttgt ctgttgttct
     9001 tgttcctccg gtgttatcga gttgaaacgc atcgcatcag caatatttgg ctgacggaac
     9061 tggatcaatt cagttggccg cgatggtagc gggaaaaggg gtaaaagcat ccttgctcct
     9121 taattcaaag agaaaaacta aagcccagaa gggaagccaa agaacttgag gattggttaa
     9181 acgtgctgtg caatgcgaag gtcattggga atgacttaaa ttccgtaacc tgatcccgcg
     9241 cataggtgac atcgccggta gtaaccggga ataccgtcat ctcattttcc agtttggtta
     9301 agccggaaga cagcaaccga taaatacgca cattgagcaa atatttagac ggtatattac
     9361 cggtaccgtc cggattgatc acccgacttt ttgccgtctt aaaccagtcc aaaacgaggc
     9421 catcaacggt atccctgacc atcattgtta tctgcccagg cgaacgctcc gttggttgaa
     9481 ggatattccc tccgccgatt ttaatcgttt catattcgat gctgtaatcg tggtaggtaa
     9541 tgtctttggc aaagaagtct gccccctcca gtccatcaac ttcgacagag aactgccatc
     9601 cttgcgcgaa cagcattttg ttcatgatga ttgacgtcag cttaccaact tcccgctcac
     9661 caacgccgga gccaaataat gtcgtcgtta atgccgaaga tacataagac tttactgaag
     9721 caacattaag ccccatatca gcccccttac ttcaacatgg atgagaaaag aacaatcccc
     9781 gggataattg cccttgttgc gctcattttc tcttccagat ccagctttcg ctgatacagc
     9841 gtgttctcgt cggataaatt gctggcatcg agtttccccg cgatagatat tcttcgcagg
     9901 cgatcagtgt taggtatcgc gattaacact tccagatagt cagaaagtaa cccaatgatt
     9961 tcaggtggca cttccccatt atccagatcc atatcacgta aattagccag atatgacaca
    10021 ttcagcgggt ataccgctcg atgagtatct tcaagctcga tattcccatc gtaaacatcg
    10081 gagtagacaa gatcgccggt atgatccgta accgatacga gcgcaagaaa atcagcaggg
    10141 caagcaagtg atttacaggt ctgatcagtg aagcgtatcc gcttgatgtg tcccgcccta
    10201 tcctggtagg ttcccaatgc ttttcttagc agggattcca gtaaggcagg ttcatccgca
    10261 atcaaaggtg tgaagcggga tttgacgtct tcgagtaatt gtcgtggtgt cattgaaacc
    10321 tcgtagaatc tagtttgtta acagattcta cgagttgtca tttgtgacag tatgctcgca
    10381 atcgtagcga ttactaccac caactgctag ctttcttctc tctagctttt ccttcgcttt
    10441 gcagtaaatc tttacttcct gtagaaatat ttctatttgc atcagcctca gactgaatta
    10501 catctgtctt agctgctctc tccttcaact cctgctctat aaaatcgttt tctcgctgga
    10561 cacgagccgc ttttgcttgc agttcgagtt tcatgctttc gagttgcagc ttgcgaagct
    10621 catcctcata agcttgatct cgcttcttgt catttgctag ctcggcttct cgtttcgctc
    10681 tacgttcatt ttctgcagca acacgctgtt tttcgcgtgc agcagcggca gatgctgctt
    10741 tttctcttct aatacgttca gcttgtgctt gttggcgaat aagttctttt ctggcatcct
    10801 cagcttgctg ctctttagct cgcccttcat tttccgcttg agcaatagca gcaatttgac
    10861 tttgcaac
//
LOCUS       DAPGRN010000138        10823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPGRN010000138 DAPGRN010000000
VERSION     DAPGRN010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.140"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            142..1284
                     /locus_tag="RHW33_004036"
     CDS             142..1284
                     /locus_tag="RHW33_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1647491.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRECYGEDTGHFGMIDAIDE
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1291..2055)
                     /gene="glcC"
                     /locus_tag="RHW33_004037"
     CDS             complement(1291..2055)
                     /gene="glcC"
                     /locus_tag="RHW33_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV1647492.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHNLIRSAVPLEMNLS"
     gene            2306..3805
                     /gene="glcD"
                     /locus_tag="RHW33_004038"
     CDS             2306..3805
                     /gene="glcD"
                     /locus_tag="RHW33_004038"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV1647493.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTEEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3805..4857
                     /gene="glcE"
                     /locus_tag="RHW33_004039"
     CDS             3805..4857
                     /gene="glcE"
                     /locus_tag="RHW33_004039"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV1647494.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFTPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4868..6091
                     /gene="glcF"
                     /locus_tag="RHW33_004040"
     CDS             4868..6091
                     /gene="glcF"
                     /locus_tag="RHW33_004040"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV1647495.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6096..6500
                     /locus_tag="RHW33_004041"
     CDS             6096..6500
                     /locus_tag="RHW33_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV1647496.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6522..8693
                     /gene="glcB"
                     /locus_tag="RHW33_004042"
     CDS             6522..8693
                     /gene="glcB"
                     /locus_tag="RHW33_004042"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV1647497.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESIPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9049..10731
                     /gene="glcA"
                     /locus_tag="RHW33_004043"
     CDS             9049..10731
                     /gene="glcA"
                     /locus_tag="RHW33_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV1647498.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKLSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2509 a         2728 c         3047 g         2539 t
ORIGIN      
        1 ctgtgtagtc tctgacctta tcgtaatcat taataaatac tatttattcg tcgtttgtat
       61 tcattgggtt gattattaag gtgccgttcg ttgctgttgc aacctgtttg tcaccgttaa
      121 ttatttgtta cccacagaac tatggaatgc gatctgctga tgattaaaat tgaaaaagta
      181 attaataaaa atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat
      241 ccaaactgga tacccccttt attcattgaa cgcaacgagc atttgtctgc gaaaaatccg
      301 gggacggacc atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg
      361 cgtattaccg cgcaaatcga taccttacac cgcgagtgtt acggcgaaga taccggtcat
      421 ttcggcatga ttgacgccat tgatgagccg caggtttttg ccgcgttgtt tggcgcagcg
      481 gaagcgtggt tgaagtcaca aggtgcaagt aagatcagcg ggcctttcag cctgaatatc
      541 aatcaggaaa gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca
      601 cacggcaaac cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat
      661 ttactggcgt ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg
      721 atggatcagg tgcgcaaaaa ggtgaccatt cgctgcatca atcgccagcg gtttgccgaa
      781 gagatgcaga tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg
      841 ccgtttaccg aacatgaatt cgcgaccatg ggcgaccaac ttaaatatct ggtgccggac
      901 gatatgatct acatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg
      961 aacatcaacg aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa
     1021 ttgctgtggc gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg
     1081 cgcgacgagt atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc
     1141 ttacgcgatc cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa
     1201 accaatacag gcatgaataa catgctggaa cgcattgggg cagagccgta caagcgttac
     1261 cgattgtatg aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc
     1321 cgagcgaatc aaattgtggc cttccagctc gatatcgtgg agattctttt tcacggtccg
     1381 cacatgatcg cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta
     1441 gatccgcgca tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt
     1501 tacgctggca aacactgaat taaacatcag gtcggtcaat gattgcagcg taaacaccag
     1561 cacctgattg tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg
     1621 ttcgatcagc gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg
     1681 ggttatcaca acaaaatcag cctgcgttcc cagcgttgcc gcaagccttg ccgattcgcc
     1741 ctccagcaat gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tgctgaacag
     1801 atgaattagc gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc
     1861 ctgcgccgtt tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga
     1921 gcgtgagaag ccgagctttt cacacagtcg acgttccgag ggaagcggct gaccgacctt
     1981 cagtacgccg tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg
     2041 acgttcatct ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac
     2101 tcgcaaaaca cgcacatcac gtaagtgatt atgttacatc aatttaacat tgagttaacc
     2161 aagacaaggt cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga
     2221 aaacgaaaag cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca
     2281 caacacaaca aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc
     2341 tttacccgat gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact
     2401 ggagatcctg cataccgagg aggagatcat tccttacgag tgtgacgggt tgagcgcgta
     2461 tcgcacgcgt ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct
     2521 ggctgtctgc catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc
     2581 tggtggcgcg ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat
     2641 cctcgacatt aaccccgttg ggcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc
     2701 gatctcccag gccgttgcac cgcataatct ctactacgca ccggaccctt catcacaaat
     2761 cgcctgttcg attggcggca atgtggctga aaatgccggc ggcgtccact gcctgaaata
     2821 tggtctgacc gtacataacc tgctgaaaat tgaagtgcaa acgctggatg gcgaggcgct
     2881 gacgctggga tcggatgcgc tggattcgcc tggttttgac ctgctggcgt tgttcaccgg
     2941 atcggaaggt atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc
     3001 cgtggcgcgg gttctgttag caagctttga ctcggtagaa aaagccggac ttgcggttgg
     3061 tgacatcatc gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat
     3121 ccgcgcggcg gaagatttta ttcatgccgg ttatcccgtc gacgccgaag cgattttgtt
     3181 atgcgagctg gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat
     3241 cttgttgaaa gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg
     3301 tttctgggcc ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta
     3361 ctgcatggat ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg
     3421 tttatcgcag caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat
     3481 gcacccgtta atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagtt
     3541 gggcgggaag atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg
     3601 catcgggcga gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac
     3661 cttccatgcg gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat
     3721 tcccacgcta caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc
     3781 tttccctgaa ctggagcgtt tctgatgcta cgcgagtgtg attacagtca ggcgctgctg
     3841 gagcaggtga atcaggcaat tagcgataaa acgccgctgg tgattcaggg cagcaatagc
     3901 aaagcctttt taggtcgccc agtcaccggg caaacgctgg atgtgcgttg tcatcgcggc
     3961 attgttaatt acgacccgac cgagctggtg ataaccgctc gtgccggaac gccgctggtg
     4021 gcgattgaag cggcgctgga gagcgccggg caaatgcttc cctgcgagcc gccgcattat
     4081 ggtgaagaag ccacctgggg cgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg
     4141 tggagcggtt cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cactggaaaa
     4201 catctgcgtt ttggtggcga agtgatgaag aacgttgccg gatacgacct ctcacggtta
     4261 atggccggaa gctacggttg tcttggcgtg ctcactgaaa tctcaatgaa agtgttaccg
     4321 cgaccgcgcg cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc catgagcgaa
     4381 atcgccgagt ggcaactcca gccattaccc attagtggct tatgttactt cgacaatgcg
     4441 ttgtggatcc gccttgaggg cggcgaagga tcggtaaaag cagcgcgtga actgctgggt
     4501 ggcgaagagg ttgcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc
     4561 tcgttaccag gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta
     4621 cccggcgagc aactgatcga ctggggcggg gcgttacgct ggctgaaatc gacagccgat
     4681 gacaatcaaa tccatcgcat cgcccgcaac gctggtggtc atgcgacccg ctttagtgcc
     4741 ggagatggtg gctttacccc gctatcggct cctttattcc gctatcacca gcagcttaaa
     4801 cagcagctcg acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga
     4861 gcaggctatg caaacccaat taactgaaga gatgcggcag aacgcgcgcg cgctggaagc
     4921 cgacagcatc ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta
     4981 tcagcttctg ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt
     5041 gctggaaggc aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gcctcacttg
     5101 ccgcaattgt gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg
     5161 gcgtgatatt gtcgagcaga aagtgaaacg cccactgccg gagcgaatac tgcgtgaagg
     5221 attgcgccag gtagtgccgc gtccggcggt cttccgtgcg ctgacgcagg tagggctggt
     5281 gctgcgaccg tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc
     5341 taaaccgcgt ccgccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca
     5401 gcctacgctt tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat
     5461 cagcgtcatg tcagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc
     5521 gcaggagaaa gggctggcgc gggcgcgcaa taatattgat gcctggtggc ccgcgattga
     5581 agcaggtgcc gaggcaattt tgcaaaccgc cagcggctgc ggtgcgtttg tcaaagagta
     5641 cgggcagatg ctgaaaaacg atgcgttata tgccgataaa gcgcgtcagg tcagtgaact
     5701 ggcggtcgat ttagtcgagc ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg
     5761 cgataaaaaa ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg
     5821 cgaagtggaa aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca
     5881 tctgtgctgc ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct
     5941 gcgggataac aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa
     6001 cattggttgc cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga
     6061 aattgtagaa caagcccttg aaaaggaata aaaaaatgaa aactaaagtc attcttagcc
     6121 agcaaatggc gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt
     6181 ctgtttccat tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg
     6241 attgcgcgcc gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc
     6301 gtcgtgaaac taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg
     6361 cgccgttatt aacgtcgctg gaaggcggcg taccggttgt tgttgatggg caaattattg
     6421 gtgccgtggg cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag
     6481 cagcggtgtt ggcgaaataa gcgaaaacga ggagagaaac aatgagtcaa accataaccc
     6541 agggccgttt acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttaccgg
     6601 gaacagggct ggacgctgcg gcgttctggc gcaattttga tgagatcgtt cacgatctgg
     6661 caccagaaaa tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt
     6721 ggcatcgcag caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg
     6781 aactgggcta cctggtgccg caaccggagc gcgtgacggt ggaaaccacg ggcattgata
     6841 gcgaaatcac cagccaggcg gggccacagc tggtggttcc ggcaatgaac gcccgctacg
     6901 cgctgaacgc ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca
     6961 tcatcccgca ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt gagcaggtta
     7021 tcgcctgggt tcggcgtttc ctcgatgaat ctataccgct ggaaaacggc agctatcagg
     7081 atgtggtggc gtttaaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag
     7141 aaaccacgtt acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc gctgcgccga
     7201 cctgcatttt gctgaaaaat aacggcctgc atattgagct gcaaatcgat gccaacgggc
     7261 ggattggcaa agacgatccg gcgcacatca acgatgttat cgtcgaagcg gccatcagta
     7321 ccattctcga ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt
     7381 accgcaatct gctgggcctg atgcagggga ctctgcaaga gaaaatggag aaaaacggtc
     7441 gacaaatcgt gcgtaaactg aatgacgatc gtcattacac cgccgccgat ggctctgaaa
     7501 tttctctgca cggacgctcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc
     7561 ctgtgatttg ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga
     7621 ctggcgcgat tgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct
     7681 atattgtgaa accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta
     7741 cccgcattga gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg
     7801 aagaacgtcg gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc aaccgcgtgg
     7861 cgttcatcaa taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag
     7921 ctggcccgat gctgcgtaaa aatcagatga aatcgacgcc ttggatcaaa gcctacgagc
     7981 gtaataacgt gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag
     8041 gcatgtgggc aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc
     8101 gtgccggggc aaacacagcc tgggttccgt cgccaactgc cgctacgctc catgcgctgc
     8161 actaccacca aaccaacgta cagagcgtgc aagccaacat tgcccagacc gagttcaatg
     8221 ctgaatttga accgctgctg gacgatctgc tgactattcc ggttgctgaa aacgctaact
     8281 ggtcggcgca agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg
     8341 tggtgcgctg ggtggagcag gggattggtt gttcaaaagt gccggatatt cacaatgtgg
     8401 cgctgatgga agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac
     8461 gtcacggtat tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg
     8521 ttgatcagca aaacgctggc gatccggctt atcgtccgat ggcggggaat ttcgctaact
     8581 cgtgtgcttt taaagctgcc agcgatttga tcttcctcgg cgtgaaacag ccaaacggct
     8641 ataccgaacc gttattacat gcctggcgtt tgcgcgaaaa agaaagtcat taatagcgtc
     8701 aaaaaaagcc ccggcaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt
     8761 atcccggaaa taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat
     8821 tactagcggg cctaaaacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc
     8881 ggaacagaaa cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc
     8941 gtactgttgc tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta
     9001 caaccttaag tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg
     9061 acccaaatgt atatgccgat gggaggactg gggctatccg ctctggttgc cctgatcccg
     9121 ataatattct tcttcgttgc actcgcggta ttacgtctga aaggacatgt tgccggagcg
     9181 ataaccctta tattatctat cctgattgcg atattcgcct ttaaaatgcc gattgatatg
     9241 gcatttgctg ctgcgggcta tggctttatt tatggattat ggccaatagc gtggattatt
     9301 gtcgcggcgg tgttcctcta taaattaacc gttgccagcg ggcagttcga tattatccgc
     9361 agctcggtta tctccatcac tgacgatcag cgtttgcagg tgttactgat tggtttctcc
     9421 tttggcgcgc tgctggaagg ggcggctggc tttggtgccc cggtggcgat taccggtgcg
     9481 ctgctggtgg gcctgggctt caaaccttta tacgcggcag ggttgtgtct gattgccaac
     9541 actgcgccgg tggcgtttgg tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg
     9601 ggaatcgatc cgttccacat tggtgctatg gcaggacgtc agttaccgtt cctgtcggtt
     9661 cttgtgccgt tctggctggt agcaatgatg gacggctgga aaggggtgaa agagacgtgg
     9721 ccagcggcgc tggttgcagg gggaagcttc gctgtcactc agttctttac ctctaactat
     9781 attggtccgg aactgccgga tattacttcg gcgctggtga gtatcgtctc actcgcttta
     9841 ttccttaaag tctggcggcc gaaaaatacc gaaacggcaa tcagcatggg acaatccgca
     9901 ggtgcgatgg tggtaaataa gctatcttct ggcggtcccg tgccttcaga atatagtctg
     9961 gggcaaatca ttcgagcgtg gtcaccgttt ttaatcttaa cggtgctggt caccatctgg
    10021 accatgaagc cgtttaaagc gttatttgct ccgggcggcg cgttttattc actggtgatt
    10081 aatttccaga tccctcattt gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa
    10141 ccaacgccaa tggatgcggt gtttaaattc gaccccctct cggctggcgg caccgctatt
    10201 tttattgcgg cgattatctc tatcttcatc ctcggtgtgg ggatcaagaa aggtattggc
    10261 gtctttgccg aaacgctaat tagcttgaag tggccgatac tgtcgattgg catggtgctg
    10321 gcgttcgcct tcgtcaccaa ctattctggc atgtccacca cgctggcgct ggtactggca
    10381 ggtacaggcg tgatgttccc gttcttctca ccgtttctcg gctggctggg cgtattcctt
    10441 accggctcgg acacctcctc taacgccctg tttggttcac tgcaatcgac cacggcgcag
    10501 caaatcaacg tctctgacac tctgctggtg gcagcaaata ccagcggcgg cgtaactggc
    10561 aagatgatct ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga
    10621 gaatctgaac tgttccgcta caccgtgaag cacagtctga tttttgccag cgttatcggc
    10681 attatcacgc tgttgcaggc gtatgtgttt accgggatgt tagtctcgta aatcaataaa
    10741 gccggatgat cgttaatatc atccggcttc atttacatta ttctgtctta ttaattgaaa
    10801 attatttctc gctttattat cca
//
LOCUS       DAPGRN010000139        10739 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPGRN010000139 DAPGRN010000000
VERSION     DAPGRN010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.141"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            148..1056
                     /locus_tag="RHW33_004044"
     CDS             148..1056
                     /locus_tag="RHW33_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDV1647499.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1276..3267)
                     /gene="tkt"
                     /locus_tag="RHW33_004045"
     CDS             complement(1276..3267)
                     /gene="tkt"
                     /locus_tag="RHW33_004045"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV1647500.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3545..4303
                     /gene="loiP"
                     /locus_tag="RHW33_004046"
                     /gene_synonym="yggG"
     CDS             3545..4303
                     /gene="loiP"
                     /locus_tag="RHW33_004046"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV1647501.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4622..5542)
                     /gene="speB"
                     /locus_tag="RHW33_004047"
     CDS             complement(4622..5542)
                     /gene="speB"
                     /locus_tag="RHW33_004047"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV1647502.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5678..6409)
                     /locus_tag="RHW33_004048"
     CDS             complement(5678..6409)
                     /locus_tag="RHW33_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647503.1"
                     /translation="MKKWKVRSALIALVVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6555..8531)
                     /gene="speA"
                     /locus_tag="RHW33_004049"
     CDS             complement(6555..8531)
                     /gene="speA"
                     /locus_tag="RHW33_004049"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV1647504.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8540..8671)
                     /gene="yqgB"
                     /locus_tag="RHW33_004050"
     CDS             complement(8540..8671)
                     /gene="yqgB"
                     /locus_tag="RHW33_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV1647505.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8807..9022
                     /locus_tag="RHW33_004051"
     CDS             8807..9022
                     /locus_tag="RHW33_004051"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647506.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            9326..10480
                     /gene="metK"
                     /locus_tag="RHW33_004052"
     CDS             9326..10480
                     /gene="metK"
                     /locus_tag="RHW33_004052"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV1647507.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2669 a         2863 c         2703 g         2504 t
ORIGIN      
        1 atttatttat tgaatgttcg aaaacatact tttctaatgc aatgtttttt cgctataccc
       61 ctttatagaa tctgaattct cacctgtcgg ctgttttctc ctcatctcat gtgacatatt
      121 cccacacttt actcaaggaa gattgtcatg aaagcacctg tttcattaac ctcatttcgc
      181 ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct gccatttctc
      241 tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg aaaaatcgta
      301 aaagttgccg agccgggtct gggttttaag atcccgttta tggaaagcgt ggagaaaatt
      361 tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg cgaccagcaa
      421 ccggcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc aggcgcggtt
      481 tataccacct acaacaccat tgaagcatta aaagatcgtc tgattgtccg tcagttgcca
      541 actcaactgg agaacgtttt cggccaatat accgccatca gcgctgtaca ggatcgtaca
      601 aaactggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc agtggtaatt
      661 gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc gattgaagac
      721 cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac agagaaaatc
      781 caggcgcaaa tagcggtaac tcaggcccag gcagaagcag acagtaaact ggcggcagcc
      841 aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac tattcgttta
      901 aaaagtgcgg ctgaagcaga agcaatccgc ctgcgcggtg aagctctgcg ggataatccc
      961 ggacttgtag ccctcaccac tgccgaacgt tgggacggta aactgcccga taccatgatc
     1021 cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt tcgcggccct
     1081 tgagagtgat ggcaaaagca aaactgcctg atgcgctacg cttatcaggc ctacatactt
     1141 cctgcaatat attgaatttg cacgatcttg taggtcggat aaggcgttca cgccgcatcc
     1201 gacatgaaca acgcgcactt taccaataat ctaaagggcc gctttcgcgg cccttctcac
     1261 aaaacgtctt actgattaca gcagcgcttt tgctttcgca acaacgttat caacagtgaa
     1321 gccgaactct tcaaacagca gctctgccgg agcagattca ccgaaggtgg tcataccgac
     1381 gatagcaccg ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctacagc
     1441 aacgcgtgca gtaaccgctt tcggcagtac agactcacgg taagccgcat cctgcttgtc
     1501 aaatgcgtcg gtagacggca tggacaccac gcgcgctttc acgccttcag cagtcagttt
     1561 ttcgtaggca gcaaccgcca gttcaacttc tgaaccggta gcgatgaaga tcagttccgg
     1621 ctgaccagca cagtctttca gcacataacc accgcgcgcg atgtttgcca gttgctcttc
     1681 agtacgttcc tgctgcgcca ggttctgacg ggagaggatc agcgcggtcg ggccgtcctg
     1741 acgctcaaca ccgtatttcc acgctaccgc ggattcaacc tggtcacacg gacgccatgt
     1801 agacatgttc ggggtcacac gcagagaagc gacctgctct accggctggt gagtcgggcc
     1861 atcttcgccc agaccgatgg agtcgtgggt gtaaaccatc acctggcgct gtttcatcag
     1921 cgcagccata cgtacggcgt tacgtgcgta ttcgacaaac atcaggaagg tggaggtgta
     1981 cggcaggaag ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg
     2041 aacaccgtag tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca
     2101 cagggtcagg ttagacggtg ccaggtcagc agagccgccg aggaattcag gcaacagcgg
     2161 gccgaacgct tcgatagcat tctgcgacgc tttacggctg gcgattttcg ccggattagc
     2221 ctgcagttta gcgataaact ctttcgcttt cgcgtcgaag tcagacggca tttcgccttt
     2281 catacggcgg gtaaattcag ctgcttcctg cggataagct ttcgcgtaag ccgcaaactt
     2341 ctcattccat gcggattctt tcgcctggcc tgcttctttc gcatcccact gagcatagat
     2401 ttcagacggg atttcgaacg gcgcgtattt ccagcccagc tgttcgcggg tcagagcgat
     2461 ttctgcatca cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggcgaacc
     2521 gaaaccgatg atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc
     2581 ttctactgcg cgtttgatgg atgctgcgtc atgaccgtcg atgtcgcgaa taacgtgcca
     2641 gccgtaagct tcgaaacgca ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc
     2701 aatagagata ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc
     2761 cagagagcaa acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta
     2821 ggtgtagtgg tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc
     2881 tgcaatcgcc atacctactg cgttggcaat accctgcccc agcggaccgg tggtggtttc
     2941 cacaccagcg gtgtaaccca cttccgggtg gcccggagtt ttagagtgca gctgacggaa
     3001 gtttttcagt tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag
     3061 catggagccg tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg
     3121 cgggttgtgt ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg
     3181 ggcacccggg tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc
     3241 attggcaagc tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc
     3301 cttaacgact tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc
     3361 gcaagccgcg cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat
     3421 gctcgcatgt tgcgcaatct actcgcccgt ccgctgcgct tttccttata ctgagactga
     3481 gcgtcggttc acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaaa
     3541 aaacatgaaa attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgactggttg
     3601 ccagaatatg gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag
     3661 tttgagtgat gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa
     3721 ggcgacgatt gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc
     3781 gctaggcaac aatatcaacg gtcagccggt aaattacaaa gtgtatatgg cgaaggatgt
     3841 gaacgccttt gcaatggcta acggctgtat ccgtgtctat agcgggctga tggatatgat
     3901 gacggataac gaagtcgaag cggtgatcgg tcacgaaatg gggcacgtgg cgttaggcca
     3961 tgtgaaaaaa ggaatgcagg tggcacttgg tacaaatgcc gtgcgagtag ctgcggcctc
     4021 tgcgggcggg attgtcggca gtttatctca atcacaactc ggtgatctgg gcgagaaatt
     4081 agtcaattcg caattctccc agcgccagga agcagaagcc gatgattatt cttacgatct
     4141 tctgcgccaa cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcaaa
     4201 actggaagaa ggtcgccaaa gctcaatgtt tgacgaccat cctgcatccg ccgaacgcgc
     4261 ccagcatatt cgcgatcgta tgagcgcgga cgggattaag taaggcatgg tggtgccaaa
     4321 cgtagttcag ataaggcatt tacgccgcat ctgacataaa tggcacattc actacctcgt
     4381 gcgcattgcc ggatgcgatg ctggcgcatc ttatccggcc tacgggtgcc aaacgtaggt
     4441 cagataaggc gttcacgccg catctgacat gaatggcaca tttgttacct tgtgcgcatt
     4501 gccggatgcg atgctggcgc atcttatccg gcctacgggt gccgaacgta ggtcggataa
     4561 ggcgttcacg ccgcatccga cattaatggc acgttttacc cgtgcgcatc gcatccggcg
     4621 cttactcgcc ctttttcgcc gcctgaatat acagcatttc cagcgccagc gttgccgctg
     4681 ccagagcagt gatttccgac tgatcgtatg ccggagccac ttccactacg tccatcccaa
     4741 cgatgttgag atctttcagg ccgcgtacca gtttaatagc gcgatcggag gtcaggccgc
     4801 caatcaccgg cgtaccggtg cctggtgcaa aagcaggatc caggcagtcg atatcaaagg
     4861 tcaggtaaac cggcatatca cccacaattt gtttcacctg ggcgataacg tcatccacgc
     4921 tgcgatcgtt cacctggcag gcgtccagca cggtaaagcc gttgtcttta tcgaactcag
     4981 tacgaatacc aatctgcacg gaatgattcg ggtcgattaa accttctttt ggcgcggtat
     5041 agaacatggt gccgtggtca aattcacaac cgttcgcgta ggtatcggtg tgggcgtcaa
     5101 agtgtaccag cgccattttg ccgaaatgct tcgcatgagc acgcagcagc ggcagcgtaa
     5161 caaagtggtc accaccgaag gagagcatac gcttaccggc agccagcagc ttctcggcgt
     5221 gtgcctgcag tttttcgctc atctcacggg catcgccgaa ggcatatacc agatcgccgc
     5281 agtccaccac gttcagacgc tcgcgcatgt cgaaattcca cgggaagcgg ttgtgttccc
     5341 aggccaggtt agtcgaaacc tggcgaatcg ccgccgggcc gtgacgacca ccggcacggc
     5401 cagaggtggc catgtcgaac ggcacgccgg tgatcaccca gtcggcatcg ctgtcatacg
     5461 gctggaagtt catcggcagg cgtaaaaaac caaaggcgtt ggaaaccagt gagttatcgt
     5521 attgatgacc taaggtgctc atggatggac tcctattaca aagtcgatat aaaaaaaccc
     5581 ttccgcgtcg ttaggcccga cgaggaaggg ttggatttgc gaaacacaag tttgtgctaa
     5641 ttatcgcctt aaatgggcga ggcatcaagc gaggttgcta tttccgacac ggtggcggcg
     5701 catagccatt gttatctgcc ggaaaggctt gtccagagca cggtggcagc gcgttttgcg
     5761 cctggccttg taattcaaag gcggcaaagc ctattacccc ggtattacgc acatcgcctt
     5821 gcgccgaatt ggctgcgtag gaatcagcaa cgttggcgaa ctgaaaagcc gcttcggtat
     5881 gtttatcttt gcggaatcct ttaatcgcta acgaatctcc ggcattcaca atatagccgt
     5941 tgttattcag cgaaccttgc ttgccgttca gcacatccag gccatcaacc gtagcaacga
     6001 tctcgtagtt ggtgttacga ctgtaattac ggacatataa ttgataattc attccgactc
     6061 tggcatggac aatatagcgt ggttcattat tggatgaatc ataaacacgc gtaataggta
     6121 gagaattaaa attaccatcg cgaatagcat attccagttc gttaatacga atgctataaa
     6181 cgcggtcgta gccggaagga acattagtcg aataactaat aacgatcatc tccgccggat
     6241 cgcgccaggc tcgctcagca gatacgccct gaaccgttga gtgcacatcc cccccccagg
     6301 cggtgccgac attgcccgat gcactgctat tatactgcgc attactggaa caaccggcca
     6361 gcagcacgac taatgctatc aacgcgctac gtactttcca ttttttcata accgttccct
     6421 ctgcatttaa ttaatgcagc gattgtaggg agattattta ataagaaaag cagctaaatt
     6481 atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc gacccgccag
     6541 cggaataatc ttaattattc atcttcaaga taggtataac cgtacaaacc tgcctcgaac
     6601 tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac ttgatcgcgg
     6661 aactgggtta acagcgtttt cggatcgagc tgcacatatt gcagcatgtc cgccacggta
     6721 tcaccttcgt cagacagttc cacttcgaca ctaccgtcag ggaacacgaa cacgtcaacc
     6781 gcttcggtat caccgaacag gttgtgcatg ttgccgagga tctcctgata tgcgccgacc
     6841 ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcatagg cattgtcgtg
     6901 gcaataccat caccatcgat atagtggtcg atagcaccgt cagagtcaca ggtaatatcc
     6961 agcagcacag cgcggcgttc cggcacttga tccagccctt ccagcggcag aaccgggaac
     7021 aactggtcga tcccccacgc atccggcatc gactggaaca gcgagaagtt gacgtacatt
     7081 ttgtccgcca tacgttcctg cagctcgtcg ataatcggac gatgagcacg gttttgcgga
     7141 tccagctgct tttgcacttc atggcacatg ctcaaataaa gctgctcagc ccatgcgcgt
     7201 tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag atccatctga
     7261 ctgtcgtgta accattcacg cagagaacgg cgagttcccg gttcgtgcat ctcctgccag
     7321 gtttcccaca tgctttgcag cgcgcgcggc gcatcttctg caggcgcggt cggcaccgtg
     7381 tattcgttac gttccacgcc gatgatatta gacaccagca cggtgtgatg cgcagtcact
     7441 gcacgacccg attcagtgat taccgtcgga tgcggcagac cattttcttc acacgcatca
     7501 ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac ggagcagtca
     7561 gactgcgaac gggtgccttc atagtccacg cccagaccgc cgccgacgtc gaagcactga
     7621 atattgacgc ccagcttgtg cagttccaca tagaaacgcg cggattcacg aacgcccgtg
     7681 gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag ttgcaggctg
     7741 tcgagacgcc cagcttcacg cagcgtttca accagttgca gtacctgagt cgcagccagg
     7801 ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg cgaagccaga
     7861 cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc cagcacaatg
     7921 gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt ctcgccaatt
     7981 aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac gctacgggtc
     8041 atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc cagacccagt
     8101 ggttcgcccg aatgaatcag ggactcgatc acgcggcggt gctggttaac tttgatcgga
     8161 taaacaagga agtaatcgcc gttatagccg taggattccc gcgcacgttt gaacgcggcg
     8221 ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc aggcagacgc
     8281 tggccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc ttccgggacg
     8341 tccgggtccg ggcacacgct aatgtggccc agctcgttaa cgtcatagta gttattgccc
     8401 caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct cattgcaacc
     8461 tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc catagacatg
     8521 tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta acgactatcg
     8581 cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt gaatgctgac
     8641 gctccaactg cgcaaccggt ttctttttca taacattatt aagcacataa ccgaacgtaa
     8701 gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg cccttgttca
     8761 gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg gtataacatc
     8821 ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca gaacggtgag
     8881 acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc cgcctgttaa
     8941 gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa cacgaaagaa
     9001 acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa tggcagaaaa
     9061 ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca gccacgtttt
     9121 taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt gacctaaaat
     9181 agccatccag atgttaatcc atccataccg attaacactc agactgccag tgtttttaac
     9241 ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt tgagctaacc
     9301 aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg agtccgtctc
     9361 tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag acgcgatcct
     9421 cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg gcatggtttt
     9481 agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca cccgtaacac
     9541 cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact cctgtgcggt
     9601 tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc gtgccgaccc
     9661 gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta atgaaactga
     9721 cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc aggctgaagt
     9781 gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg tgactttcca
     9841 gtatgacgac ggcaaaatcg ttggtatcga tgctgtcgtg ctttccactc agcactctga
     9901 agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca agccgattct
     9961 gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg gtcgttttgt
    10021 tatcggtggc ccgatgggtg actgcggtct gactggtcgt aaaattatcg ttgataccta
    10081 cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat caaaagtgga
    10141 ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg ctggcctggc
    10201 cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga cttccatcat
    10261 ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc tggtacgtga
    10321 gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc acccgatcta
    10381 caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg aaaaaaccga
    10441 caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca cctgctttca
    10501 aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag gcgttcacgc
    10561 cgcatccggc aaaaaaaccg cctgcacaat aacatcattc ttcctgatca cgtttcaccg
    10621 caaattatca tcacaactga aaccgattac accaaccaca acagacaaat atttgtaata
    10681 ttttcatatt attattcggt tttcacagtt gttacatttc ttttcagtaa agtcttaat
//
LOCUS       DAPGRN010000140        10676 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPGRN010000140 DAPGRN010000000
VERSION     DAPGRN010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.142"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            291..695
                     /gene="sdhC"
                     /locus_tag="RHW33_004053"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             291..695
                     /gene="sdhC"
                     /locus_tag="RHW33_004053"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV1647508.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            689..1036
                     /gene="sdhD"
                     /locus_tag="RHW33_004054"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             689..1036
                     /gene="sdhD"
                     /locus_tag="RHW33_004054"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV1647509.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            1036..2802
                     /gene="sdhA"
                     /locus_tag="RHW33_004055"
     CDS             1036..2802
                     /gene="sdhA"
                     /locus_tag="RHW33_004055"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV1647510.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            2818..3534
                     /gene="sdhB"
                     /locus_tag="RHW33_004056"
     CDS             2818..3534
                     /gene="sdhB"
                     /locus_tag="RHW33_004056"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV1647511.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEADEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNEQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            3835..6636
                     /gene="sucA"
                     /locus_tag="RHW33_004057"
     CDS             3835..6636
                     /gene="sucA"
                     /locus_tag="RHW33_004057"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV1647512.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            6651..7868
                     /gene="odhB"
                     /locus_tag="RHW33_004058"
     CDS             6651..7868
                     /gene="odhB"
                     /locus_tag="RHW33_004058"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV1647513.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            8234..9400
                     /gene="sucC"
                     /locus_tag="RHW33_004059"
     CDS             8234..9400
                     /gene="sucC"
                     /locus_tag="RHW33_004059"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV1647514.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            9400..10269
                     /gene="sucD"
                     /locus_tag="RHW33_004060"
     CDS             9400..10269
                     /gene="sucD"
                     /locus_tag="RHW33_004060"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV1647515.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
BASE COUNT         2522 a         2787 c         2983 g         2384 t
ORIGIN      
        1 cctggatcac tgttcaggat aaaacccgac aaactatatg taggttaatt gtaatgattt
       61 tgtgaacggc ctatactgcc gccaggtctc cggaacaccc tgcaatcccg agccacccag
      121 cgttgtaacg tgtcgttttc gcatctggaa gcagtgtttt gcatgacgcg cagttataga
      181 aaggacgctg tctgacccgc aagcagaccg gaggaaggaa atcccgacgt ctccaggtaa
      241 cagaaagtta acctctgtgc ccgtagtccc cagggaataa taagaacagc atgtgggcgt
      301 tattcatgat aagaaatgtg aaaaaacaaa gacctgttaa tctggactta cagaccatcc
      361 ggttccccat cacggcgata gcgtccattc tccatcgcgt ttccggtgtg atcacctttg
      421 ttgcagtggg tatcctgctg tggcttctgg gtaccagcct ctcttcccct gaaggtttcg
      481 agcaagcttc cgcgattatg ggcagcttct tcgtcaaatt tatcatgtgg ggcatcctta
      541 ccgctctggc gtatcacgtc gtcgtaggta ttcgccacat gatgatggat tttggctatc
      601 tggaagaaac attcgaagcg ggtaaacgct ccgccaaaat ctcctttgtt attactgtcg
      661 tgctttcact tctcgcagga gtcctcgtat ggtaagcaac gcctccgcat taggacgcaa
      721 tggcgtacat gatttcatcc tcgttcgcgc taccgctatc gtcctgacgc tctacatcat
      781 ttatatggtc ggttttttcg ctaccagtgg cgagctgaca tatgaagtct ggatcggttt
      841 cttcgcctct gcgttcacca aagtgttcac cctgctggcg ctgttttcta tcttgatcca
      901 tgcctggatc ggcatgtggc aggtgttgac cgactacgtt aaaccgctgg ctttgcgcct
      961 gatgctgcaa ctggtgattg tcgttgcact ggtggtttac gtgatttatg gattcgttgt
     1021 ggtgtggggt gtgtgatgaa attgccagtc agagaatttg atgcagttgt gattggtgcc
     1081 ggcggcgcag gtatgcgcgc ggcgctgcaa atttcccaga gcggccagac ctgtgcgctg
     1141 ctctctaaag tcttcccgac ccgttcccat accgtttctg cgcaaggcgg cattaccgtt
     1201 gcgctgggta atacccatga agataactgg gaatggcata tgtacgacac cgtgaaaggg
     1261 tcggactata tcggtgacca ggacgcgatt gaatatatgt gtaaaaccgg gccggaagcg
     1321 attctggaac tcgaacacat gggcctgccg ttctcgcgtc tcgatgatgg tcgtatctat
     1381 caacgtccgt ttggcggtca gtcgaaaaac ttcggcggcg agcaggcggc acgcactgcg
     1441 gcagcggctg accgtaccgg tcacgcactg ttgcacacgc tttatcagca gaacctgaaa
     1501 aaccacacca ccattttctc cgagtggtat gcgctggatc tggtgaaaaa ccaggatggc
     1561 gcggtggtgg gttgtaccgc actgtgcatc gaaaccggtg aagtggttta tttcaaagcc
     1621 cgcgctaccg tgctggcgac tggcggggca gggcgtattt atcagtccac cactaacgcc
     1681 cacattaaca ctggcgacgg tgtcggcatg gctatccgcg caggcgtacc ggtgcaggat
     1741 atggaaatgt ggcaattcca cccgaccggc attgccggtg cgggcgtact ggtcaccgaa
     1801 ggttgccgtg gtgaaggcgg ttatctgctg aacaaacatg gcgaacgctt tatggagcgt
     1861 tatgcgccga acgccaaaga cctggcgggc cgtgatgtgg tggcgcgttc catcatgatc
     1921 gaaatccgtg aaggtcgcgg ctgtgatggt ccgtgggggc cacacgcgaa actgaaactc
     1981 gatcacctgg ggaaagaagt tctcgaatcc cgtctgccgg gtatcctgga gctttcccgt
     2041 accttcgctc acgttgatcc ggtgaaagag ccgattccgg ttatcccaac ctgtcactac
     2101 atgatgggcg gtattccgac caaagttacc ggtcaggcgc tgactgtgaa tgagaaaggc
     2161 gaagatgtgg ttgttccggg gctgttcgcg gtgggtgaaa ttgcctgtgt atcggtacac
     2221 ggcgctaacc gtcttggcgg taactcgctg ctggacctgg tggtctttgg tcgcgctgct
     2281 ggcctgcatc tgcaagagtc tatcgccgag cagggcgcac tgcgcgatgc cagcgagtct
     2341 gatgtagaag cgtctctgga tcgcctgaac cgctggaaca acaaccgtaa cggtgaagat
     2401 ccggtggcga tccgtaaagc gctgcaagaa tgtatgcagc ataacttctc ggtcttccgt
     2461 gaaggtgatg cgatggcgaa agggcttgag cagttgaaag ttatccgcga gcgtctgaaa
     2521 aatgcccgtc tggatgacac ttccagcgag ttcaacaccc agcgcgttga gtgtctggaa
     2581 ctggataacc tgatggaaac ggcgtatgca acggctgttt ctgccaactt ccgtaccgaa
     2641 agccgtggcg cgcatagccg cttcgacttc ccggatcgtg atgatgaaaa ctggctgtgc
     2701 cactccctgt atctgccaga gtcggaatcc atgacgcgcc gaagcgtcaa catggaaccg
     2761 aaactgcgcc cggcattccc gccgaagatt cgtacttact aatgcggaga caggaaaatg
     2821 agactcgagt tttcaattta tcgctataac ccggatgttg atgatgctcc gcgtatgcag
     2881 gattacaccc tggaagcgga tgaaggtcgc gacatgatgc tgctggatgc gcttatccag
     2941 ctgaaagaga aagatcccag cctgtcgttc cgtcgctcct gtcgtgaagg tgtgtgcggt
     3001 tccgacggtc tgaacatgaa cggcaagaat ggtctggcct gtattacccc gatttcggca
     3061 ctcaaccagc cgggcaagaa gattgtgatt cgcccgctgc caggtttacc ggtgatccgc
     3121 gatttggtgg tagacatggg acaattctat gcgcaatatg agaaaattaa gccttacctg
     3181 ttgaataatg aacaaaatcc gccagctcgc gagcatttac agatgccaga gcagcgcgaa
     3241 aaactcgacg ggctgtatga atgtattctc tgcgcatgtt gttcaacctc ttgtccgtct
     3301 ttctggtgga atcccgataa gtttatcggc ccggcaggct tgttagcggc atatcgtttc
     3361 ctgatcgata gccgtgatac cgagactgac agccgcctcg acggtttgag cgatgcattc
     3421 agtgtattcc gctgtcacag catcatgaac tgcgtcagtg tatgtccgaa ggggctgaac
     3481 ccgacgcgcg ccatcggtca tatcaagtcg atgttgttgc aacgtaatgc gtaaaccgta
     3541 ggcctgataa gacgcgcaag cgtcgcatca ggcaaccagt gccggatgcg gcgtgaacgc
     3601 cttatccggc ctacaagtca ttacccgtag gcctgataag cgcagcgcat caggcgtaac
     3661 aaagaaatgc aggaaatctt taaaaactgc ccctgacact aagacagttt ttaaaggttc
     3721 cttcgcgagc cactacgtag acaagagctc gcaagtgaac cccggcacgc acatcactgt
     3781 gcgtggtagt atccacggcg aagtaagcat aaaaaagatg cttaagggat cacgatgcag
     3841 aacagcgctt tgaaagcctg gttggactct tcttacctct ctggcgcaaa ccagagctgg
     3901 atagaacagc tctatgaaga cttcttaacc gatcctgact cggttgacgc taactggcgt
     3961 tcgacgttcc agcagttacc tggtacggga gtcaaaccgg atcaattcca ctctcaaacg
     4021 cgtgaatatt tccgccgcct ggcgaaagac gcttcacgtt actcttcaac gatctccgac
     4081 cctgacacca atgtgaagca ggttaaagtc ctgcagctca ttaacgcata ccgcttccgt
     4141 ggtcaccagc atgcgaatct cgatccgctg ggactgtggc agcaagataa agtggccgat
     4201 ctggatccgt ctttccacga tctgaccgaa gcagacttcc aggagacctt caacgtcggt
     4261 tcatttgcca gcggcaaaga aaccatgaaa ctcggcgagc tgctggaagc cctcaagcaa
     4321 acctactgcg gcccgattgg tgccgagtat atgcatatca ccagcaccga agaaaaacgc
     4381 tggatccaac agcgtatcga gtctggtcgc gcgactttca atagcgaaga gaaaaaacgc
     4441 ttcttaagcg aactgaccgc cgctgaaggc cttgaacgtt acctcggtgc aaaattccct
     4501 ggtgcaaaac gcttctcgct ggaaggcggt gacgcgttaa tcccgatgct taaagagatg
     4561 atccgccacg ctggcaacag cggcacccgc gaagtggttc tcgggatggc gcaccgtggt
     4621 cgtctgaacg tgctggtgaa cgtgctgggt aaaaaaccgc aagacttgtt cgacgagttc
     4681 gccggtaaac ataaagaaca cctcggcacg ggtgacgtga aataccacat gggcttctcg
     4741 tctgacttcc agaccgatgg cggcctggtg cacctggcgc tggcgtttaa cccgtctcac
     4801 cttgagattg taagcccggt agttatcggc tctgttcgtg cccgtctgga cagacttgat
     4861 gagccgagca gcaacaaagt gctgccaatc accatccacg gtgacgccgc agtgaccggg
     4921 cagggcgtag ttcaggaaac tctgaacatg tcgaaagcgc gtggttatga agttggcggt
     4981 acggtacgta tcgttatcaa caaccaggtt ggcttcacca cctctaaccc gctggatgcc
     5041 cgttctacac cgtactgtac tgatatcggt aagatggttc aggcaccgat tttccacgtt
     5101 aacgcggatg atccggaagc cgttgccttt gtgacccgtc tggcgctcga tttccgtaac
     5161 acctttaaac gtgatgtctt catcgacctg gtgtgctacc gccgtcacgg ccacaacgaa
     5221 gccgacgagc cgagcgcaac ccagccgctg atgtatcaga aaatcaaaaa acatccgacg
     5281 ccgcgcaaaa tctacgcaga caagctggag caggaaaaag tggcgacgct ggaagatgcc
     5341 accgagatgg ttaacctgta ccgcgatgcg ctggatgctg gcgattgcgt agtggcagag
     5401 tggcgtccga tgaacatgca ctctttcacc tggtcgccgt acctcaacca cgaatgggac
     5461 gaagagtacc cgaacaaagt tgagatgaag cgcctgcagg aactggctaa acgcatcagt
     5521 acggtgccgg aagcggttga aatgcagtct cgcgttgcca agatctatgg cgaccgccag
     5581 gcgatggctg ccggtgaaaa actgttcgac tggggcggcg cggaaaacct cgcttacgcc
     5641 acgctggttg acgaaggcat tccggttcgc ctgtcgggtg aagactccgg tcgcggtacc
     5701 ttcttccacc gccacgcggt gatccacaac cagtctaacg gttccactta cacgccgttg
     5761 caacatatcc ataacggcca gggcgcgttc cgtgtctggg actccgtact gtctgaagaa
     5821 gcggtgctgg cgtttgaata tggttatgcc accgcagaac cacgcactct gactatctgg
     5881 gaagcacagt ttggtgactt cgccaacggt gctcaggtgg ttatcgacca gttcatctcc
     5941 tctggcgaac agaaatgggg ccggatgtgt ggtctggtga tgttgctgcc gcacggttac
     6001 gaagggcagg ggccggagca ctcctccgcg cgtctggaac gttatctgca actttgcgct
     6061 gagcaaaaca tgcaggtgtg cgtaccgtct accccggcac aggtttacca catgctgcgt
     6121 cgccaggcgc tgcgcgggat gcgtcgtccg ctggtcgtga tgtcgccgaa atccctgctg
     6181 cgtcatccgc tggcggtttc cagcctcgaa gaactggcga acggcacctt cctgccagct
     6241 atcggtgaaa tcgacgagct tgatccgaag ggcgtgaagc gcgtagtgat gtgttctggt
     6301 aaggtttatt acgacctgct ggaacagcgt cgtaagaaca atcaacacga tgtcgccatt
     6361 gtgcgtatcg agcaactcta cccgttcccg cataaagcga tgcaggaagt gttgcagcag
     6421 tttgctcacg tcaaggattt tgtctggtgc caggaagagc cgctcaacca gggcgcatgg
     6481 tactgcagcc agcatcattt ccgtgaagtg attccgtttg gggcttctct gcgttatgca
     6541 ggccgcccgg cctccgcctc tccggcggta gggtatatgt ccgttcacca gaaacagcaa
     6601 caagatctgg ttaatgacgc gctgaacgtc gaataaataa aggatacaca atgagtagcg
     6661 tagatattct ggtccctgac ctgcctgaat ccgtagccga tgccaccgtc gcaacctggc
     6721 ataaaaaacc cggcgacgca gtcgtacgtg atgaagtgct ggtagaaatc gaaactgaca
     6781 aagtggtact ggaagtaccg gcatcagcag acggcattct ggatgcggtt ctggaagatg
     6841 aaggtacaac ggtaacgtct cgtcagatcc ttggtcgcct gcgtgaaggc aacagcgccg
     6901 gtaaagaaac cagcgccaaa tctgaagaga aagcgtccac tccggcgcaa cgccagcagg
     6961 cgtctctgga agagcaaaac aacgatgcgt taagcccggc gatccgtcgc ctgctggctg
     7021 aacacaacct cgacgccagc gccattaaag gcaccggtgt gggtggtcgt ctgacccgtg
     7081 aagatgtgga aaaacatctg gcgaaagccc cggcgaaaga gtctgctccg gcagcggctg
     7141 ctccggcggc gcaaccggct ctggctgcac gtagtgaaaa acgtgtcccg atgactcgcc
     7201 tgcgtaagcg tgtggcagag cgtctgctgg aagcgaaaaa ctccaccgcc atgctgacca
     7261 cgttcaacga agtcaacatg aagccgatta tggatctgcg taagcagtac ggtgaagcgt
     7321 ttgaaaaacg ccacggcatc cgtctgggct ttatgtcctt ctacgtgaaa gcggtggttg
     7381 aagccctgaa acgttacccg gaagtgaatg cttctatcga cggcgatgac gtggtttacc
     7441 acaactattt cgacgtcagc atggcggttt ctacgccgcg cggcctggtg acgccggttc
     7501 tgcgtgatgt cgataccctc ggcatggcag acatcgagaa gaaaatcaaa gagctggcag
     7561 tcaaaggccg tgatggcaag ctgacggttg aagatctgac cggtggtaac ttcaccatca
     7621 ccaacggtgg tgtgttcggt tccctgatgt ctacgccgat catcaacccg ccgcagagcg
     7681 caattctggg tatgcacgct atcaaagatc gtccgatggc ggtgaatggt caggttgaga
     7741 tcctgccgat gatgtacctg gcgctgtcct acgatcaccg tctgatcgat ggtcgcgaat
     7801 ccgtgggctt cctggtaacg atcaaagagt tgctggaaga tccgacgcgt ctgctgctgg
     7861 acgtgtagta gtttaagttt cacctgcact gtagaccgga taagacatta tcgccttctc
     7921 cggcaattga agcctgatgc gacgcttaac gcgtcttatc aggcctacgg gaagcaccga
     7981 tgtaggtcgg ataaggcgct cgcgtcgcat ccgacaagcg atgcctgatg cgacgcttac
     8041 acgtcttatc aggcctacgg ggagcaccga tgtaggtcgg ataaggcgct tgcgccgcat
     8101 ccgacaagcg atgccagatg cgacgcttac acgtcttatc aggcctacgg gtgaccgaca
     8161 atacccggaa gcgatacgaa atattcggtc tacggtttaa aagataacga ttactgaagg
     8221 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     8281 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     8341 aaaaatcggt gccggtccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     8401 agcgggcggt gtgaaagttg taaacagtaa agaagacatc cgtgcttttg cagaaaactg
     8461 gctgggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
     8521 tctggttgaa gcggcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
     8581 tagttcacgt cgcgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
     8641 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
     8701 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
     8761 gttcaccaaa atctttatgg gcctggcgac cattttcctg gagcgtgacc tggcgctgat
     8821 cgaaatcaac ccgctggtca ttaccaaaca gggcgatctg atttgcctcg acggcaaact
     8881 gggcgctgat ggcaacgccc tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
     8941 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
     9001 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
     9061 taaactgcac ggcggcgaac cagctaactt ccttgacgtt ggcggcggcg caaccaaaga
     9121 acgtgtaacc gaagcgttca aaatcattct ctctgacgac aaagtgaaag ccgttctggt
     9181 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
     9241 agcggaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
     9301 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
     9361 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
     9421 aacaccaagg ttatctgcca gggctttacc ggtagccagg ggactttcca ctcagaacag
     9481 gccattgcat acggaactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
     9541 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
     9601 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agccatcgac
     9661 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
     9721 gtgaaagtga aactggacga agcaggcgtt cgtatgatcg gcccgaactg cccaggcgtt
     9781 atcaccccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
     9841 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
     9901 tacggtttcg gtcagtcgac ctgtgtcggt atcggcggtg acccgatccc tggctctaac
     9961 tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    10021 ggtgagatcg gcggtagcgc tgaagaagaa gcagctgcgt acatcaaaga gcacgttacc
    10081 aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    10141 gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    10201 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    10261 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    10321 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttat gccttaaacg
    10381 gttaaccacg gtcgaaatct gactgcaggc ttgactgcat gaccacagcc actgtggatt
    10441 gccgcaaccg accgcaagcc acgaataaga acgacggcga atttcgaatc gttacctgat
    10501 gcactggctt cccagcgttt gaccgtagcg ttaagtaaat caacaaaagc gatgttcacc
    10561 gcttttcgtc aagtcgtgcc atttgtcagc caggatcggg tctttacccc ggatattgca
    10621 gcatcgcaaa aattcttaga gggatatgct ataaatcaaa aataaaatct ttgttg
//
LOCUS       DAPGRN010000141        10518 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPGRN010000141 DAPGRN010000000
VERSION     DAPGRN010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.143"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..2965
                     /locus_tag="RHW33_004061"
                     /pseudo
     CDS             81..2965
                     /locus_tag="RHW33_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
     gene            complement(3065..4984)
                     /gene="dhaR"
                     /locus_tag="RHW33_004062"
     CDS             complement(3065..4984)
                     /gene="dhaR"
                     /locus_tag="RHW33_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV1647516.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAVL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5212..6282
                     /gene="dhaK"
                     /locus_tag="RHW33_004063"
     CDS             5212..6282
                     /gene="dhaK"
                     /locus_tag="RHW33_004063"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV1647517.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVTG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6293..6925
                     /gene="dhaL"
                     /locus_tag="RHW33_004064"
     CDS             6293..6925
                     /gene="dhaL"
                     /locus_tag="RHW33_004064"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV1647518.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            6936..8354
                     /gene="dhaM"
                     /locus_tag="RHW33_004065"
     CDS             6936..8354
                     /gene="dhaM"
                     /locus_tag="RHW33_004065"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV1647519.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGAEIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGEMEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSAVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            8674..10371
                     /gene="treA"
                     /locus_tag="RHW33_004066"
     CDS             8674..10371
                     /gene="treA"
                     /locus_tag="RHW33_004066"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV1647520.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPT
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTIRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWQFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPVKQKEAEPTP"
BASE COUNT         2765 a         2648 c         2613 g         2492 t
ORIGIN      
        1 gataagagga tattattcat tgacttaaac aaaatgacaa aatccataga cccatagcct
       61 gggtactata taggagtggt atgggcatca aacaacacaa tgggaatacc aaagccgatc
      121 gtctcgctga attaaaaatc cgttcgccct caattcaact gataaaattt ggcgctattg
      181 gtttgaatgc aattatcttt tcccccctgc tgatagctgc tgatacagga agtcaatatg
      241 gcaccaatat tactattaat gatggtgaca gaattacagg agataccgcc gatccatcag
      301 gaaacctcta tggtgtaatg accccagcag gaaacacgcc tggcaatatc aacctgggta
      361 atgatgtcac cgtcaatgtc aacgacgcct ctggatatgc aaaaggaatc attattcagg
      421 gcaaaaacag ctccctgaca gctaaccgac tcacagtaga tgttgttggt caaacctctg
      481 ccatcggcat taatttaatt ggtgactata cccatgctga cttaggcaca ggcagcacca
      541 ttaagagtaa cgatgacggc atcattattg ggcatagctc aacactaaca gccactcaat
      601 tcaccattga aaactcgaac ggtataggcc taaccatcaa tgactatggc accagtgtcg
      661 atcttggaag cggaagtaaa atcaagaccg atggaagtac aggtgtttat atcggtggtc
      721 tcaacggcaa taacgccaat ggtgctgcgc gtttacggcg acagacctga caatcgatgt
      781 tcagggctac agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac
      841 aaacagttcc attaaaacca gtggcgataa tgcacacggc ctctggaact ttggccaggt
      901 tagcgcgaat gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg
      961 tggtggtaca accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggtct
     1021 cgtcaccagt ggttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag
     1081 catcttttcc ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat
     1141 gcaaaatacc gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcact
     1201 cagcggcggt agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagggg
     1261 gatttatgcc atgaccaaca gtcagatcga cctcacgagc gatctggtca ttgatatgag
     1321 tacacccgac cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat
     1381 caacgcttcg ggtcgtatgc ttatcaacgg tagcgttctc tccaaaggtg ggctaatcaa
     1441 tctggatatg cacccagggt cggtttggac aggttcctcc cttagcgata atgtcaatga
     1501 cgggaaactg gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct
     1561 cgacacgctg gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg
     1621 cacatttacc acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc
     1681 tgatgttgtt ggcgagggta atggcgttaa taataaaggg gatttattga atatcagcgg
     1741 gagtagtgct ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg
     1801 aaatgaagtt ctgacagtgg taaaaaccac tgacggtgcg gcctcgttca gcgcgtcttc
     1861 tcaggttgag ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct
     1921 ttacgcttcc gggacagttc cagaaccgac tcctaatcct gaaccaacac cagctcccgc
     1981 tcagcctccc atagtcaacc ccgatcctac gcctgaaccc gatcctacgc ctaaccccac
     2041 tcccacacct aaacccacca cgaccgcaga tgctggcggc aattatctca atgtcggtta
     2101 cttattaaac tatgttgaaa accgtacgct gatgcaacga atgggtgacc tgcgaaatca
     2161 gagtaaagac ggtaatatct ggctgcgcag ttatggggga agcctggact ccttcgccag
     2221 tggcaaactg agcggctttg acatgggtta cagcggtatc cagtttggtg gggataaacg
     2281 tctctctgat gtaatgccgt tgtatgtcgg tctgtatatt ggctcaacac atgcatcgcc
     2341 agactatagc ggtggcgacg gtaccgcacg ttcagactac ataggaatgt acgccagtta
     2401 catggcacac aacggttttt acagcgatct cgttgtaaaa gcatcgcgcc agaaaaatag
     2461 tttccacgta ctggacagtc agaacaacgg cgttaacgcc aacggcactg cgaatggatt
     2521 gagcatctcc ctggaagccg ggcagaggtt caacctgacc cctactggtt atgggtttta
     2581 tatagagccg caaacccagc tcacatacag ccaccagaat gagatggcta tgaaggcgag
     2641 taatggtctc aatatacatc tgaatcacta cgaatcgctg ctggggcgtg ccagcatgat
     2701 actgggatat gacatcaccg caggtaaaag ccagctgaat atctatgtga agactggcgc
     2761 tatccacgag ttttcagggg atacagaata tctgttgaac aactcccggg agaagtacag
     2821 tttcaaaggt aatggctgga ataacggcgt gggagtcagt gcacagtata acaaacagca
     2881 cacattctac ctcgaagcgg attacacgca gggtaacctc tttgatcaga agcaagtcaa
     2941 cggaggatat cgcttcagct tttaagtgcg ggatagacaa gcccttaaac gatgctgaaa
     3001 aatgttagcg tgcgccggaa tgaaatatag atcactctga tgtatcagaa atcgtttctg
     3061 tctttcatac ccggcgctta aactgccctg catcaatgcc atgttgcttc attttccgcc
     3121 acagcgtagt gcggcctatt ccaagtaaag ccgacatttc ctgaatgcga ccgcctgtga
     3181 cctgggctgc gttaataatt gcctcttttt caacttccgc aaatgacaga ctggtggaaa
     3241 ggcgtgtggc gctgacatca tctgttgcct gctcggtaaa cagatgttcc ggcaaatcac
     3301 tgacgcgaat gcgcccatta tcactactca gagcaagatt ctcgatgacg ctgtaaagtt
     3361 caaaatcgtt gccaggccat gcacaggaaa ccaggcgagc gagggcatca tcatcaattt
     3421 tcagacgcgt agagaagcgt ttttcaagac tgcgtaattt gttattcacc agcgccggaa
     3481 tgctgccacg ccgcatacgc agaggcggga tggtaatttc aaatgcatgc agcgcgtaat
     3541 acagctggcg actaaaacga ttttgttcca ccagcattgc gaggtccgcg gtcgttgtag
     3601 caatcacttt gacatcaatt ggtattaaac gccgcgcatc cagtcgcgtg ataacaccct
     3661 gcttgataac ctgaagcaaa gcagactgta actccaccgc cagatattca atcttttcaa
     3721 gaaacagcgt gccgccgtgc gccagttcca gccgactcag acggccattt tcattgtccg
     3781 tgcgatcgcc gccaataaat tcttccgcca gcaccgcatc accatataac tcacaattga
     3841 cggcgatata aggacctgca gcacgctcgc tttcattatg aattgcctga cttagcagtg
     3901 ccttgcccac gccttcttct ccacaaagca ggacaggaaa gctactgcgc gccgcctggc
     3961 gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg tggcatatga gcgaaggtat
     4021 ggctgacttt tcctaattga ctggtcatta actgccgcat ctgttccaca ggatggagca
     4081 acaaaataaa gctggttccc tgcgtttcta ttatcggttt aagggttatc accgcatcaa
     4141 taaactggtg ctgactttca aaggttgctt ctacgtgttt gagcggatgt gcctgtttta
     4201 ttgcttgttg caatacggcg ggtaacgtta agagttcagt gattgcccgt ccctgacttg
     4261 ccgtcgcgtc aaggcgcaag acccgcgccg cctgggcatt aataaattgc aaattaccct
     4321 gctcgtccca gctaatcacg ccatcatcca tactttctaa cagggcatta agttgattta
     4381 aatgacggtt agtttcagcg agcaaactgt ccgtcagcag taaatttccg acctcgcggg
     4441 cgattgccaa cgtcaacggc aaatcagctg cggtagtttg ctcaaccgga cacgccagcg
     4501 ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg cgttgcacaa aaggcccagt
     4561 tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt cacggcctga ccagagatag
     4621 ccgctaacga tagcgcacaa gtaccaataa ttccctcggc gcaatacgtg ccgtcattga
     4681 atcccagtgc acttagctgc tgcaaggttt gcggatcgcc attacggctg agaatgcagg
     4741 cggtttcatc gaggataaac agcgcacact ctcgcggtgc catatattcc caggcatctt
     4801 ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg ccgataaata gaagcaaatg
     4861 tcacgccctg ggcctgatgt ggtacgttcc atgtctcccg tttcatcagc ttattgcatc
     4921 gctcccagga ggttgcaatt aagggagata tgccccgacc atcgttgtta aaagcgccac
     4981 tcatatcccg catcccttat gacaaaaggt gttccggctc gcaattttaa gtattaatac
     5041 acgtaaaagt ttcaatatgg aacattcatt aaagcatatg tttcactatg aaacataatt
     5101 tgggctaatt ttcttttccc ttgcctgatg cacaatggat tcatcggcag tcagtggtcg
     5161 ccgtgtcgtt gaacatcatc catgccctac cgtaattgct ggagcaaaat aatgaaaaaa
     5221 ttgatcaatg atgtgcaaga cgtactggac gaacaactgg caggactggc gaaagcgcat
     5281 ccatcgctga cactgcatca ggatccggtg tatgtcaccc gagctgatgc ccccgttaca
     5341 ggaaaagtcg ccctgctgtc gggtggcggt agcggacacg agccgatgca ctgtggttat
     5401 atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc accgacgccc
     5461 gataaaatct ttgaatgcgc catgcaaatt gatggcggcg aaggtgtact gttgattatc
     5521 aaaaattaca ccggcgatat tcttaacttc gaaacagcaa ccgagttact gcacgatagc
     5581 ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg cagtaaaaga cagtctttat
     5641 accgccgggc gacgcggcgt tgccaacacc gtattaattg aaaaactcgt aggcgcagcg
     5701 gcggagcgtg gcgactcact ggacgcctgt gcggaactgg ggcgtaagtt gaataatcaa
     5761 ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg caaaccttct
     5821 tttaccctgg cggataatga gatggagttt ggcgtcggca ttcatggtga gccgggtatt
     5881 gaccgccgcc ccttctcttc ccttgatcaa accgtcgatg aaatgttcga caccttgctg
     5941 gaaaatggct cataccatcg cactttgcgt ttctgggatt atcaacaagg cagctggcag
     6001 gaagaaccac aaaccaaaca accgctccag tctggcgaca gggtgattgc gctggttaac
     6061 aatcttggcg caactccact ttctgagctg tacggcgtct ataaccgcct aaccacacgt
     6121 tgccagcaag cgggattgac tatcgagcgt aatttaattg gcgcgtactg tacctcactg
     6181 gatatgaccg gtttctcaat caccttactg aaagttgatg acgaaacgct ggctctctgg
     6241 gacgccccgg tccacacccc ggcccttaac tggggtaaat aaggagaaag caatgtcact
     6301 gagcagaact caaattgtta actggctcac tcgttgtggc gatattttca acaccgagag
     6361 cgagtatctt accggactgg atcgcgagat tggcgacgct gaccacgggc taaatatgaa
     6421 ccgaggcttt agcaaagtgg tggaaaaact ccctgctatt gcagataaag atatcggttt
     6481 cattctcaag aataccggta tgacgctgct ttccagcgtc ggtggtgcca gtggtccgct
     6541 gttcggtacc ttctttatcc gcgcagcaca ggcgacccag gcacggcaaa gcctgacgct
     6601 ggaagagctt tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc gcgggaaagc
     6661 cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat cattacgtca
     6721 gtccagcgag caaaatctct ctgttccggc ggcgctcgaa gctgccagta gcatcgccga
     6781 atccgctgca caaagtacga ttacgatgca agcccgcaaa ggccgcgcca gttatctcgg
     6841 tgaacgcagt attggtcacc aggatcccgg cgcgacctcg gtgatgttta tgatgcaaat
     6901 gttggcgtta gccgcaaaag agtaaggaat tggtgatggt aaacctggtc atagtttcac
     6961 atagcagccg actgggagaa ggtgtcggtg aattagcccg tcagatgtta atgagtgata
     7021 attgtaaaat cgccattgct gcgggaattg acgatccaca aaatcccatt ggtaccgatg
     7081 ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg ctggtcatga
     7141 tggatatggg tagcgcatta ttgagtgctg aaactgcgct ggaattgctg gctcccgaga
     7201 tcgctgcaaa agtacgtttg tgtgctgcac cgttggtcga aggtacactg gcagcaacgg
     7261 tcagcgcggc ctcgggggcg gagatcgaca aagttatctt tgacgccatg catgcgctgg
     7321 aagccaaacg tgaacaactg ggtttaccgt cctccgacac tgaaatctct gacacatgtc
     7381 ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat taaaaaccgt aacggcctgc
     7441 acgtccgtcc ggcctcccgg ctggtttata ccttatcgac atttaatgcc gatatgttgc
     7501 tggaaaaaaa cggcaaatgc gtcacaccgg agagtattaa ccagattgcg ttactacaag
     7561 ttcgctataa cgatacgctg cgcctgattg cgaaagggcc agaagctgaa gaggcactga
     7621 tcgctttccg tcagctggct gaagataact ttggtgaaat ggaggaagtt gctccaccta
     7681 ctctgcgtcc cgttccgcct gtttcgggta aagcctttta ttatcaaccg gttttatgta
     7741 cggtacaggc caaatcaacc ctgaccgtgg aagaagaaca agaacgatta cgccaggcca
     7801 ttgacttcac gttattagat ctgatgacgt taacagcgaa agcagaagcc agcgggcttg
     7861 acgatattgc cgcaatcttt tctggtcacc atacactgtt agatgatccg gaactgctgg
     7921 cggcggcaag cgaactcctt cagcacgaac attgcacggc agaatatgcc tggcagcaag
     7981 ttcttaaaga acttagccag caataccagc aactggatga tgaatatcta caagctcgct
     8041 atattgatgt ggacgatctt ctgcatcgca ccctggtcca cctgacccaa acgaaagaag
     8101 aactcccgca gtttaactcg ccaactattc tactggcgga gaacatttat ccctccgcag
     8161 tactgcaact ggatccggcg gttgtaaaag gtatctgcct tagcgccgga agtccggtat
     8221 cccacagcgc cctgatcgcc cgtgaactgg ggattggctg gatttgccag cagggtgaga
     8281 aactgtatgc gatacaacca gaagaaaagc taacgctgga cgttaaaacg caacgtttca
     8341 accgtcaggg ttaagccacc gttcggatgg catcgttctg atgtcatccg gcaatttact
     8401 cagcactaat gttttttaac tacctgttat atttttcacc aatttatgat gatttttctt
     8461 cgctaatcgc gaaccaaacg ccgctggctg gctaataaac agacgttatt tgatcactct
     8521 atttcgccgt tatttatccc tttaattccc ttttctaaaa tgcctgacag ttcgcagaat
     8581 gagatttcga tcatgcagct agtgcgatcc tgaactaagg ttttctgata cttgaatacc
     8641 gttttattcg gtttcgccta aggagaatga ttgatgaaat cccccgcacc ttctcgcccg
     8701 caaaaaatgg cgttaattcc agcctgcatc tttttgtgtt tcgctgcgct atcggtgcag
     8761 gcagaagaaa caccggtaac gccacagccg cctgatattt tattagggcc gctgtttaat
     8821 gatgtgcaaa acgccaaact ttttccggac caaaaaacct ttgccgatgc cgtgccgaac
     8881 agcgatccgc tgatgatcct tgctgattat cggatgcagc aaaaccagag cggatttgat
     8941 ctgcgccatt tcgttaacgt caatttcacc ctgccgacag agggcgagaa atatgttccg
     9001 ccagagggac agtcactgcg cgaacatatt gacggacttt ggccggtatt aacgcgttct
     9061 accgaaaaca ccgaaaaatg ggattctctg ttaccgctgc cggaacctta tgtcgtgccg
     9121 ggcggacgtt ttcgcgaggt atattactgg gacagttact tcaccatgtt aggacttgcc
     9181 gaaagcggtc actgggataa agtcgcggat atggtggcca attttgctca tgaaatagac
     9241 acttacggtc atattcccaa cggcaaccgc agttactatt taagccgctc gcagccgccc
     9301 ttctttgccc tgatggtaga gttactggcg cagcatgaag gcgatgccgc attgaagcaa
     9361 tacctgccgc aaatgcaaaa agaatatgct tactggatgg acggtgttga aaacctgcaa
     9421 gccggacaac aggaaaaacg cgttgtaaaa cttcaggatg gtacccttct caaccgctac
     9481 tgggacgatc gtgatacgcc acgaccagag tcatgggtgg aagatattgc caccgccaaa
     9541 agcaatccga atcgacctgc cactgaaatt taccgcgacc tgcgctctgc cgctgcgtct
     9601 ggctgggatt tcagctcgcg ctggatggac aatccgcagc agttaaatac catacgcacc
     9661 accagcatcg taccggtcga tctgaacagc ctgatgttta aaatggaaaa aatcctcgcc
     9721 cgcgccagca aggctgccgg agataacgcg atggcaaacc agtacgaaac gctggcaaat
     9781 gcccgtcaaa aagggatcga aaaatacctg tggaacgatc aacaaggctg gtatgccgat
     9841 tacgacctga aaagtcataa agtgcgcaat cagttaaccg ctgccgccct gttcccgcta
     9901 tacgtcaatg cggcagcgaa agatcgcgcc aacaaaatgg cgacggcgac gaaaactcat
     9961 ctgctgcaac caggcggcct gaacaccacg tcggtgaaaa gtgggcaaca atgggatgcg
    10021 ccaaatggct gggcaccgtt gcagtgggtc gcgacagaag gattacaaaa ctacgggcaa
    10081 aaagaggtgg cgatggacat tagctggcaa ttcctgacca atgttcagca cacctatgac
    10141 cgggagaaaa agctggtgga aaaatatgat gtcagcacca ccggaacggg gggcggcggt
    10201 ggcgaatatc cattacagga tggctttggc tggaccaacg gcgtgacgct gaaaatgctg
    10261 gatttgatct gcccgaaaga gcaaccgtgt gacaatgttc cggcgacgcg tccactcagt
    10321 gaatcaacaa cacagccggt aaaacaaaaa gaggcggaac ctacgcctta actttgctgt
    10381 atatcgaggc tgtatatcga gcaatgccat tatgtgcata aaattggcat tgctcttttt
    10441 tcgccacgaa atgcccccca ttctacggtc tatgccttta ttttctttta tcagagaaga
    10501 ttttaatttt attttatc
//
LOCUS       DAPGRN010000142        10329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPGRN010000142 DAPGRN010000000
VERSION     DAPGRN010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.144"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            89..469
                     /gene="crcB"
                     /locus_tag="RHW33_004067"
     CDS             89..469
                     /gene="crcB"
                     /locus_tag="RHW33_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV1647521.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(849..2042)
                     /locus_tag="RHW33_004068"
     CDS             complement(849..2042)
                     /locus_tag="RHW33_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647522.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            2178..3902
                     /gene="iucA"
                     /locus_tag="RHW33_004069"
     CDS             2178..3902
                     /gene="iucA"
                     /locus_tag="RHW33_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDV1647523.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3903..4850
                     /gene="iucB"
                     /locus_tag="RHW33_004070"
     CDS             3903..4850
                     /gene="iucB"
                     /locus_tag="RHW33_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDV1647524.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4850..6592
                     /gene="iucC"
                     /locus_tag="RHW33_004071"
     CDS             4850..6592
                     /gene="iucC"
                     /locus_tag="RHW33_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDV1647525.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6589..7866
                     /gene="iucD"
                     /locus_tag="RHW33_004072"
     CDS             6589..7866
                     /gene="iucD"
                     /locus_tag="RHW33_004072"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDV1647526.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            7948..10149
                     /gene="iutA"
                     /locus_tag="RHW33_004073"
     CDS             7948..10149
                     /gene="iutA"
                     /locus_tag="RHW33_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDV1647527.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2465 a         2705 c         2682 g         2477 t
ORIGIN      
        1 gtagtcaaag gatataaatt aacaatatca gtcaaatgag aacatattct gatattgtta
       61 cgataaaaaa ttattttatg aggggtacat gattaaatca ctttttgccg tcattatcgg
      121 cggttctgtt ggatgtactc tgcgctggtt actgtccaca cgctttaaca gtctttttcc
      181 aaatctacct ccaggtacac tggtggtgaa cctccttgcc gggttaatta ttggtactgc
      241 gttggcgtac tttttacgac aaccacacct tgatcctttt tggaaattaa tgataacaac
      301 gggactttgt ggaggtctga gtactttctc tacattttct gttgaagtat tcgcattact
      361 ccaggcggga aattatatat gggctttgac gtctgtactt gttcacgtca ttggctccct
      421 catcatgaca gctttaggct tttttatcat aaccatactt ttcgcatgag aattcattaa
      481 cgttacaggt ttatcaacta atgtgacgct taggatagaa tttcgctcca tggtatgcgt
      541 ttgcagtcat tacagtctcc atgacgactg taatgactgc aaacgcatta cctgacatat
      601 cagtataaac aatgaattaa cctcttggcg tattttttac atcactggcc ctcacccccc
      661 aaaattctct ctgaaaaatt gttgcggtta acgttgccaa tgtatccacc catttcagaa
      721 gacgatctgg tatcctggtg gcatgctgcg atgtgcttca ggtgagtgca ctgggcggtc
      781 agcacaggcg gtttcgacag cgaaattgca caggacgtta tgccggctgt gagtacaaca
      841 tcatagcctt acttgattgt acggtaatgc caaatacatg cagacactat ggtaaataat
      901 acagcggcaa ataatgccgt cacaatcagc gcgattgaga cacctgtttt cgacgccagt
      961 ggcaccaaca atgcgcacag cccatagcca agtgtgtgac tggccgcaag ccagcccgcg
     1021 cctgcgccgt cggccagctt atcattcagt aaaagttgat aagcgggtgt tgccagagcg
     1081 gccccaaatg acagcactgc acagcccagg taaaacaacc atagctgttc agtgagcatg
     1141 atagccagac cactactcat caacaccccg gcactcagga gcagagccac tggagtcagg
     1201 cgctgtggac ggagtaccac gaactgcgcg ataagcgcag ctattgcgga cagccccaac
     1261 aaccacgcca cctgttggct aatagtggtg gtatcagtat cgaactggcg agtaagggct
     1321 ggcgaaagtc caagctgcat catgctcatt gccgcagcca gtaacattgc gcaaagcaga
     1381 taaggcaggc aatcccgctt caggctgacg ctcttgcgct ccggtgtggg ctgtggtggt
     1441 gtgccgggca accgcagaag catcaccaca gcccacaccg gagctgccat cagcatccac
     1501 actggcgcca gagggtgaat gaccaacatt gccgccgcgc acagcggccc gaatagcctg
     1561 ccgcagctca ggccggagct gatggttgcc agagcggcca tgcgattccc ttctcccgct
     1621 ctctgcaatg cccagacctg acaggctggc accattgctg acacggtcag accgtagacg
     1681 atccgggcga tgattaatat tcccaacccc accgctgttg ttacggcacc ggtagccatc
     1741 agcacgcttc ccagcccaag cagggtaaag cttacgccat atcctcccag agcccacagc
     1801 accacagtct tactgccagt aagggaaatt tgctttcccc accatgggga agatggcaga
     1861 aacagcattg agcccagcat cagcagggca gcccatacag acagactcag atttgtctgt
     1921 atgaccagaa cagggagtac aaccagcaga ccgttctgtc cgataccgag aagaccggca
     1981 ctgaacgcca gtggccagca ggacagggtt ttttggggcg tatcttcgat cttagatgac
     2041 attgtaatgt ttcaacacca tgtattaatt gtgtttattt gtaaaattaa tttttctgac
     2101 aataacattt ctcattgata atgagaatca ttattgacat aattgttatt attttactgt
     2161 gtaggagctg tttgattatg atcctgccct ctgaaaaatc cgccacagat gtggctgcgc
     2221 agtgcttcct gaatgccttg attcgtgaaa ccaaagactg gcaactggcg gaatacccgc
     2281 cagacgaatt gattatcccg ctggacgaac agaagtcgct ccatttcaga gtggcttatt
     2341 tctccccaac ccagcatcac cgctttgcat ttcctgctca tctggtcacg gcatcaggca
     2401 gttatcctgt cgactttacc actctctccc ggcttattat tgataagctc cggcatcaac
     2461 tttttctgcc agttcccctc tgcgaaactt tccaccaacg cgtgctggaa agctacgccc
     2521 atacgcaaca gacaattgat gcccgccatg actgggccat cctgcgtgaa aaagcgttga
     2581 attttggcga ggctgagcag gcactgctga caggacacgc tttccaccct gcacctaagt
     2641 ctcatgaacc gtttaaccgc caggaagctg aacgatacct gcctgacatg gcacctcact
     2701 tcccgctgcg gtggttttcg gtggataaaa cgcaaatcgc tggtgaaagt ctgcatctta
     2761 accttcaaca gcggttgacg cgatttgccg cagaaaatgc gccccagtta ctcaacgaat
     2821 taagtgacaa tcaatggctg ttcccgctgc acccgtggca gggagaatat cttttgcagc
     2881 aagtgtggtg ccaggcactt tttgctaaag gacttatcag agacttaggc gaggccggca
     2941 cgtcgtggct gccgaccacc tcttcccgct ccctctactg tgctaccagc cgcgatatga
     3001 tcaagttctc cctgagcgtt cggctgacca actccgtccg tactctgtct gtgaaagagg
     3061 tggagcgagg aatgcgcctg gcacgtctgg cgcaaaccga cggctggcag atgctacagg
     3121 cccgcttccc tactttccgg gtaatgcagg aggacggctg ggccgggctg cgcgatctta
     3181 acggcaacat catgcaggaa agtttgtttt ccctgcgtga aaacctgctg ctggagcagc
     3241 cgcaaagcca gaccaacgtt ctggtttccc tgactcaggc cgcaccggat ggcggtgatt
     3301 cgctgctggt ttcggcggta aaacgcctga gcgatcgcct cggtatcact gtgcaacagg
     3361 ccgcccacgc atgggtcgat gcttactgtc agcaggtgct gaagcctctg tttacggctg
     3421 aagcggatta cggcctggtg ctgctggcgc atcagcaaaa tattcttgtg cagatgcttg
     3481 gggatctgcc ggtcggattt atttaccgtg actgccaggg cagcgcgttt atgcctcacg
     3541 cgacagagtg gctcgatacc attgacgagg cgcaggcgga aaatattttc acccgtgagc
     3601 agttgctgcg ctatttccct tattacctgc tggttaactc cacttttgcc gtaactgctg
     3661 cgctgggtgc tgccgggctg gacagcgaag cgaatctgat ggctcgtgta cgaacattgc
     3721 tggctgaagt gcgtgaccag gtgactcata aaacctgtct caactatgtt ctggaaagtc
     3781 cgtactggaa cgtaaaaggt aactttttct gttatctgaa cgatcataac gagaacacca
     3841 tcgttgaccc ttcggtgatc tattttgatt tcgctaaccc gctgcaggct caggaggtct
     3901 gaatgtctgg ggcaaacatt gttcacagcg gatatggact gcgctgtgaa aaactcgaca
     3961 agcctctgaa tcttggctgg gggctggaca atagcgcggt gttgcactgg cccggggagc
     4021 tgccaacagg gtggctgtgc gacgcgctgg atcagatatt tatcgccgca ccacaacttt
     4081 cagcagtggt tcttccctgg tccgaatggt gtgaggagcc acaggcgctg acgcttttcg
     4141 gacaggtaca aagcgacatt atccatcgtt ccgctttctg gcagttaccg ttatggctga
     4201 gttctccggc aaaccgggcc tccggtgaaa tggtttttga tgcagagcgt gagatttatt
     4261 tcccgcagcg ccccccccgt ccgcagggtg aagtttatcg tcgttacgat ccacgcattc
     4321 gcaggatgct gagtttccgc attgccgatc ccgtttctga tgcagaacgt ttcactcgct
     4381 ggatgaacga tccgcgcgtt gagtatttct gggagcaaag tggctcactg gaggtacaga
     4441 tcgcctatct ggaacgccag ctcaccagta aacatgcatt cccgctgatt ggttgtttcg
     4501 acgatcggcc gttcagttat ttcgaaatct actgggcggc ggaagaccgc attggtcgcc
     4561 actattcgtg gcagcccttt gaccgtggcc tgcatctgct ggttggtgaa cagcaatggc
     4621 gcggagccca ctatgtgcaa agctggctgc gcggggtgac acattacctg ctgctgaatg
     4681 agccacgtac gcagcgcaca gtactggagc cacgcaccga taaccagcgc ctgttccgcc
     4741 atcttgagcc tgcgggatac cggacaatta aagagttcga cttcccacat aaacgctcgc
     4801 gcatggtgat ggcggatcgt catcacttct tcacggaggt cggtctgtaa tgaatcacaa
     4861 agactgggat ttggtcaacc gccgcctggt ggcaaaaatg ttgtctgagc tggagtatga
     4921 gcaggttttc cacgcggaat ctcagggcga tgaccgctac tgcattaacc tgccgggagc
     4981 acaatggcgc ttcatcgctg aacgtggtat ctggggctgg ctctggattg atgctcaaac
     5041 tctgcgctgc gcggacgagc cagtactggc tcagacgctg ctgatgcagc taaagcaggt
     5101 actgtcaatg agcgatgcaa ccgttgctga gcatatgcag gatttgtatg ccacgctgct
     5161 gggcgacctg caactactga aagcccgtcg cgggctgagc gccagtgacc tgattaatct
     5221 taatgccgac cgcctgcaat gcctgctgag cggtcatcct aaattcgttt ttaataaagg
     5281 tcgccgtggc tggggtaaag aggcgctgga acgatatgcg ccagagtatg ccaacacctt
     5341 cagactgcac tggctggcgg taaaacgtga acatatgatc tggcgctgtg ataacgagat
     5401 ggatattcat cagttgttga cggccgcaat ggatccgcag gagtttgccc gcttcagtca
     5461 ggtctggcag gaaaacggac tggatcataa ctggctgccg ctgccggtac atccgtggca
     5521 gtggcagcaa aaaatcgcta ccgacttcat cgctgatttt gccgaaggca ggatggtgtc
     5581 tctcggcgag tttggcgacc agtggctcgc ccagcagtcg ctgcgtaccc tgaccaacgc
     5641 cagtcgccgg ggcgggctgg atatcaagct gccgctgacc atctacaaca cctcatgcta
     5701 ccgggggatc cctggcagat acatcgctgc cggaccactg gcttcacgct ggctacaaca
     5761 ggtttttgcg accgacgcca ccctagtgca aagcggcgca gtgatacttg gtgaaccggc
     5821 tgcaggctat gtctcccatg aaggctatgc cgcgcttgcc cgggctccct atcgctacca
     5881 ggaaatgctt ggtgttatct ggcgggagaa tccgtgccgc tggctgaaac cggatgaaag
     5941 tccggttctg atggcaacac tgatggagtg cgacgaaaac aatcagccgc tggcaggcgc
     6001 atatatagac cgctccgggc tggacgctga aacctggctt acgcaactgt tccgggtggt
     6061 cgtggttcct ctgtatcacc tgctttgccg ctacggtgtc gcgcttattg cacatggaca
     6121 aaatataact ctcgccatga aagagggggt tccacagcgt gttctgctga aagacttcca
     6181 gggcgatatg cgactggtga aagaagagtt ccccgaaatg gactctttgc ctcaggaggt
     6241 tcgtgatgtt acatcccgcc tgagtgcgga ctacttaatc catgatttgc agacgggtca
     6301 cttcgtgaca gtactgcgtt ttatttcgcc actgatggtt cgtcttggcg tacctgaaag
     6361 gcgattttat caactgctgg cagcagtgtt gagtgattac atgaaaaaac atccacaaat
     6421 gtcagagcgt tttgcgcttt tctcactttt caggccacaa atcattcgcg tggtgctgaa
     6481 cccggtaaaa ctgacctggc ctgatctgga tggcggcagc cgcatgctgc cgaattacct
     6541 tgaggatctg caaaatccgc tgtggctggt aactcaggaa tatgaatcat gaaaaaaagt
     6601 gtcgatttta ttggtgtagg gacagggcca tttaatctca gcattgctgc gttgtcacat
     6661 cagatcgaag aactggactg tctcttcttt gatgaacatc ctcatttttc ctggcatccg
     6721 ggtatgctgg taccggattg tcatatgcag accgtctttc tgaaagatct ggtcagtgct
     6781 gttgcaccta caaatcccta cagttttgtt aactatctgg tgaagcacaa aaagttctat
     6841 cgcttcctta caagcagact acgtacagta tcccgtgaag agttttctga ctacctccgc
     6901 tgggctgctg aagatatgaa taacctgtat ttcagtcata ccgttgaaaa cattgatttc
     6961 gataaaaaac gtcgattgtt tctggtgcaa accagccagg gagaatattt tgcccgcaat
     7021 atctgccttg gtacaggaaa acaaccttat ttaccaccct gtgtgaagca tatgacacaa
     7081 tcctgtttcc atgccagtga aatgaatctt cgtcggccgg accttagtgg aaaacggata
     7141 accgtggttg gtggaggaca gagtggtgca gacctgttcc ttaatgcatt acgcggggaa
     7201 tggggagaag cggcggaaat aaactgggtc tcccggcgta ataattttaa cgcactggat
     7261 gaggctgctt ttgctgatga gtattttaca cctgaatata tttcaggctt ctccggactg
     7321 gaggaagata ttcgccatca gttactggat gagcagaaaa tgacatcgga tggcatcact
     7381 gccgattctt tactgaccat ttatcgtgag ttgtaccacc gttttgaagt tctgagaaaa
     7441 ccaagaaata tccgtctgct acccagccgc tcggtaacaa ctctggaaag tagtggtcct
     7501 ggctggaagt tgctgatgga gcatcatctg gatcagggca gggagagcct ggaaagtgat
     7561 gtggtgattt tcgccacagg ttaccgttct gcgttgccac aaatacttcc ctcactgatg
     7621 cccctgatca ccatgcacga taagaacacc tttaaagtgc gtgatgactt cactctggaa
     7681 tggagtggcc cgaaagagaa caacatcttc gtggtcaacg ccagtatgca aacccatggc
     7741 atcgccgaac cccagctcag cctgatggca tggagatctg cacgtattct taatcgcgta
     7801 atgggacgtg atttattcga tctcagtatg ccgcccgccc tgattcagtg gcgcagcggc
     7861 acctagggaa aacgcagccg gaggctgctt ctttaactcg ctacacagca tctttgggct
     7921 gattttttcc gcccgtatgg aggaataatg atgataagca aaaagtatac gctttgggct
     7981 ctcaacccac tgcttcttac catgatggcg ccagcagtcg ctcaacaaac cgatgatgaa
     8041 acgttcgtgg tgtctgccaa ccgcagcaat cgcaccgtag cggagatggc gcaaaccacc
     8101 tgggttatcg aaaacgccga actggaacag cagattcagg gcggcaaaga gcttaaagac
     8161 gcactggctc agctgatccc tggccttgac gtcagcagcc ggagccgcac caactacggt
     8221 atgaatgtgc gtggccgccc gctggtcgtg ctggttgacg gcgtgcgtct caactcttca
     8281 cgtaccgaca gccgacaact ggactctata gatcctttta atatcgacca tattgaagtg
     8341 atctccggtg cgacgtccct gtacggcggc ggcagtaccg gtggcctgat caacatcgtg
     8401 accaaaaaag gccagccgga aaccatgatg gagtttgagg ctggcaccaa aagtggcttt
     8461 agcagcagta aagatcacga tgaacgcatt gccggagctg tctccggcgg aaatgagcat
     8521 atctccggac gtctttccgt ggcatatcag aaatttggcg gctggtttga cggtaacggc
     8581 gatgccacct tgcttgataa cacccagacc ggcctgcagt actccgatcg gctggacatc
     8641 atgggaactg gtacgctgaa catcgatgaa tcccggcagc ttcagttgat cacacagtac
     8701 tataaaagcc agggcgacga cgattacggg cttaatctcg ggaaaggctt ctctgccatc
     8761 agagggacca gcacgccatt cgtcagtaac gggctgaatt ccgaccgtat tcccggcact
     8821 gagcggcatt tgatcagcct gcagtactct gacagcgctt ttctgggaca ggagctggtc
     8881 ggtcaggttt actaccgcga tgagtcgttg cgattctacc cgttcccgac ggtaaatgcg
     8941 aacaaacagg tgacggcttt ctcttcgtca cagcaggaca ccgaccagta cggcatgaaa
     9001 ctgactctga acagcaaacc gatggacggc tggcaaatca cctgggggct ggatgctgat
     9061 catgagcgct ttacctccaa ccagatgttc ttcgacctgg ctcaggcaag cgcttccgga
     9121 gggctgaaca acaagaagat ttacaccacc gggcgctatc cgtcgtatga catcaccaac
     9181 ctggcggcct tcctgcaatc aggctatgac atcaataatc tctttaccct caacggtggc
     9241 gtacgctatc agtacactga aaacaagatt gatgatttca tcggctacgc gcagcaacgg
     9301 cagattgccg ccgggaaggc tacatccgcc gacgccattc ctggcggctc agtcgattac
     9361 gacaacttcc tgttcaacgc cggtctgctg atgcacatca ccgaacgcca gcaggcatgg
     9421 ctcaacttct cccagggcgt ggagctgccg gacccgggta aatactatgg tcgcggcatc
     9481 tatggtgctg cagtgaacgg ccatcttcct ctaacaaaga gtgtgaacgt cagcgacagc
     9541 aagctggaag gcgtgaaagt cgattcttat gagctgggct ggcgctttac tggcaataat
     9601 ctgcgtaccc aaatcgcggc ctactattcg atttctgata agagcgtggt ggcgaataaa
     9661 gatctgacca tcagcgtggt ggacgacaaa cgccgtattt acggcgtgga aggtgcggtg
     9721 gactacctga ttcctgatac tgactggagt accggagtga acttcaacgt gctgaaaact
     9781 gagtcgaaag tgaacggtac ctggcagaaa tacgatgtga agacagcaag cccatcaaaa
     9841 gcgacagcct acattggctg ggcaccggac ccgtggagtc tgcgcgtgca gagcaccacc
     9901 tcctttgacg tgagcgacgc gcagggctac aaggtcgatg gctataccac cgtggatctg
     9961 ctcggcagtt atcagcttcc ggtgggtaca ctcagcttca gcattgaaaa cctcttcgac
    10021 cgtgactaca ccactgtctg ggggcagcgt gcaccactgt actacagccc gggttacggc
    10081 ccagcgtcac tgtacgacta caaaggcagg ggccgaacct ttggtctgaa ctactctgtg
    10141 ctgttctgac cggtattcct ttacaacaaa ggtacgctga tatcaacatg gccgctgaca
    10201 gccaagttga tatcatataa tacacgacat aatctgtagt cagggaggat agactcttta
    10261 ctgactacag attatgtcgt gttccgtgct catttcctca aaaaaataca agaaaagaat
    10321 tagtattct
//
LOCUS       DAPGRN010000143        10295 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPGRN010000143 DAPGRN010000000
VERSION     DAPGRN010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.145"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..718
                     /gene="ssuE"
                     /locus_tag="RHW33_004074"
     CDS             143..718
                     /gene="ssuE"
                     /locus_tag="RHW33_004074"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV1647528.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAA
                     EDLLYARFDSPALNTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLHSLRGNAHA"
     gene            711..1670
                     /gene="ssuA"
                     /locus_tag="RHW33_004075"
     CDS             711..1670
                     /gene="ssuA"
                     /locus_tag="RHW33_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV1647529.1"
                     /translation="MRNIIKLALVGLLSVSTIVVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLLYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQIGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1667..2812
                     /gene="ssuD"
                     /locus_tag="RHW33_004076"
     CDS             1667..2812
                     /gene="ssuD"
                     /locus_tag="RHW33_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV1647530.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLIPQGEAVANDFIPRKVAQS"
     gene            2824..3615
                     /gene="ssuC"
                     /locus_tag="RHW33_004077"
     CDS             2824..3615
                     /gene="ssuC"
                     /locus_tag="RHW33_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV1647531.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3612..4379
                     /gene="ssuB"
                     /locus_tag="RHW33_004078"
     CDS             3612..4379
                     /gene="ssuB"
                     /locus_tag="RHW33_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV1647532.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAAHRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELESEVLQRVMQRGESETRLRKQG"
     gene            complement(4422..7034)
                     /gene="pepN"
                     /locus_tag="RHW33_004079"
     CDS             complement(4422..7034)
                     /gene="pepN"
                     /locus_tag="RHW33_004079"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV1647533.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTACKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7300..8502
                     /gene="pncB"
                     /locus_tag="RHW33_004080"
     CDS             7300..8502
                     /gene="pncB"
                     /locus_tag="RHW33_004080"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV1647534.1"
                     /translation="MTQFASPVLYSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8672..10072
                     /gene="asnS"
                     /locus_tag="RHW33_004081"
     CDS             8672..10072
                     /gene="asnS"
                     /locus_tag="RHW33_004081"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV1647535.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2315 a         2649 c         2773 g         2558 t
ORIGIN      
        1 gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgagc gaattgttat caatgttaac aaaacaagaa
      121 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      181 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tggatgtaga
      241 ggtttatcac tggaatctgc aaaacttcgc cgcggaagat ctgctttatg ctcgtttcga
      301 tagtccggca ctcaatacct tcaccgaaca gttgcaacag gctgacgggc tgattgtcgc
      361 cacgcctgtg tataaagccg cttattccgg tgcgctgaaa accctgctcg acctgctgcc
      421 agaacgtgcg ttgcaaggca aagtggtgct accgctggcg acgggcggta cggtggccca
      481 tctgctggcg gtcgattatg cccttaagcc agttttaagt gcgctgaaag cccaggagat
      541 cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca aaccccagtt
      601 cacgccgaat ttacaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      661 ccgccgcgat gttcaggttc ctgatcttca ttctctgcga ggtaatgccc atgcgtaaca
      721 tcattaaact ggcgctggtg ggattgctta gcgtctctac gattgtggtt gctgcagaat
      781 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtattggcaa
      841 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcgtgg gtggagttcc
      901 ccgctggccc gcaaatgctt gaggcgttaa acgttggcag tattgatctc ggcagtaccg
      961 gggatattcc gccaatcttc gcccaggctg ccggggctga tttgctgtac gtgggcgtcg
     1021 agccgccgaa gccaaaagcc gaggtgattc tggtggcaga aaacagcccg atcaaaaccg
     1081 tagccgatct taaaggccac aaagttgcgt tccagaaagg ttccagttca cacaatcttt
     1141 tactgcgcgc actacgccag gccggactta agtttactga catccagccc acttatctga
     1201 cgccagctga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1261 atccctacta ctccgctgca ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg
     1321 atctcaatca aatcggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg
     1381 cttttattca gggtgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1441 gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1501 cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag gttgccgcct
     1561 tacagcagca aacggcagat ctgttttatg aaaaccgtct ggtgccgaaa aaagtcgata
     1621 ttcgccaacg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1681 gttctggttt ttaccgaccc atggtgacgg gcattatctg ggaacggaag aaggttcacg
     1741 cccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggttatac
     1801 cggtgtgctg atcccaacgg ggcgctcctg cgaagatgcg tggctggttg ccgcatcgat
     1861 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgtcccagcg taacctcacc
     1921 taccgttgcc gcccgccagg ccgccacgct tgaccgtctc tcaaatggac gtgcgttgtt
     1981 taacctggtc acaggcagcg atccacaaga actggcaggc gacggcgtgt tccttgatca
     2041 tagtgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgtt tattgctggg
     2101 ggaaaccgtc gatttcaacg gtaaacatat tcatgtgcgg ggagccaaac tgctcttccc
     2161 gccgattcaa cagccgtatc ctccgcttta ctttggcggt tcgtcagacg tcgcccagga
     2221 gctggcggca gaacaggttg atctctacct cacctggggc gaaccgccgg aactggttaa
     2281 agagaaaatc gaacaagtgc gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg
     2341 cattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2401 gttaatctcg catcttgatg atgagactat cgccaaagca caagccgcat tcgcccgcac
     2461 ggattccgtg gggcaacagc gaatggcagc gctacataac ggcaagcgcg acaatctgga
     2521 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg ggacggcgct
     2581 ggtgggcgat ggtcctacgg tcgctgcgcg aatcaacgaa tatgccgcgc ttggcatcga
     2641 cagctttgtg ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct
     2701 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccgc tgattccgca
     2761 aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc gcgcaaagct aaggaacacg
     2821 atgatggcaa cgccagtgaa gaagtggtta ttgcgcgttg ccccctggtt tttaccggtg
     2881 ggcatcgtgg cggtgtggca actggcctcc tcagttggct ggctgtcgac gcgtattttg
     2941 ccttcaccgg aaggagtggt gacggcgttc tggacgctct ccgccagcgg cgaactgtgg
     3001 cagcatctgg cgatcagctc ctggcgggcg ctgattggct tttcaattgg cggatcgctg
     3061 gggttgattt tgggactgat tagcgggcta tctcgctggg gagaacgact gctggatacc
     3121 tcaattcaga tgttgcgcaa cgtgccgcat ctggcgctga tcccgctggt gattttgtgg
     3181 tttggcattg atgaatccgc aaaaatcttt ctggtggcgc tcggtacgct attccccatt
     3241 tatatcaaca cctggcatgg catccgtaat atcgatcgcg ggttggtgga gatggcgcgt
     3301 agctatggat tatccggcat accgctgttt atccatgtga tcctgcctgg tgccctgccc
     3361 tcaattatgg tcggcgtgcg ttttgcgttg ggcctgatgt ggctgacgct gattgttgcc
     3421 gaaaccattt ctgccaattc aggcattggt tatctggcga tgaatgcgcg ggagtttttg
     3481 caaacggacg tggtggtggt cgccattatt ctttacgccc tgctcggcaa actggctgac
     3541 gtcagcgcgc agctgttaga gcgcctctgg ttgcgctgga acccggctta tcatttgaag
     3601 gaggccacgg tatgaatact gctcgtctga accagggcac gccattgttg ctcaatgcag
     3661 taagcaaaca ttacgcggaa aatatcgtcc tgaaccaact ggatttacat attccggcag
     3721 gtcagtttgt ggcggtggtg ggccgcagcg gtggtggcaa aagtaccctg ctgcgcctgc
     3781 tggcaggtct ggaaacgcca accgctggcg atgtgttagc aggcaccaca ccgctggctg
     3841 aaattcagga agacacgcga atgatgtttc aggatgcgcg tctgctaccg tggaaatcgg
     3901 tgattgataa cgttgggtta ggccttaaag gccagtggcg cgatgccgcg catcgggcgt
     3961 tggctgcggt aggactggag aatcgcgccg gggaatggcc tgccgcactt tctggcgggc
     4021 agaaacagcg agtggcgctg gcaagagcgt tgattcatcg accgggttta ttgttgcttg
     4081 atgaaccgct cggggcgctg gatgccttaa cgcgactcga gatgcaggat ttgattgtgt
     4141 cactttggca ggagcacggc tttaccgtac tgttggtgac gcatgatgtg agcgaagcgg
     4201 tagcaatggc tgaccgggtg ttattaattg aagagggaaa aattggtctg gatttgacag
     4261 tggatatccc tcgtccgcgt cgattagggt cggtgcgatt agcggagctg gaatcggaag
     4321 tgttgcaacg ggtaatgcag cgtggtgagt ctgaaactcg cctgagaaaa caggggtaaa
     4381 atccccgaat ggcggcgcta ttgccgccat tcggggattt tttaagccag tgctttggtg
     4441 atcttctcgt acagatcgcc agagagattt tccagccctt tcaactgttc cagcgccgcg
     4501 cgcattttct cctgacgttt ggcatcgtaa cgtttcaggc gaatcagcgg ttcaatcaga
     4561 cgtgaagcca cctgcgggtt acggctgttg aggtcggtaa gcatttccac caggaactgg
     4621 taaccgctcc catcttcggc atggaacgct gccgggttgc tgcccgcaaa cgcaccaatc
     4681 aacgaacgaa tacggttcgg gttgctcatg gtaaatgagc gatgctgcaa caggccgcgc
     4741 accgtctcca gcacattcgc cgccgggctg gtggcttgca ggataaacca tttatccatc
     4801 accagaccat cctgatgcca cttgtcgtcg tactcctgca tcagcgcgtc acggcaaggc
     4861 agctgtgcag caaccgccgc agaaagcgct gccagcgcat cggtcatatt gtttgcttca
     4921 tggtattgct tgctcaccag cacgtcagcc agatgcgttt caccaaaagc aaggaagcgc
     4981 aggcaggcat tacgcagagt gcgttttgca atatcttcat gctcaacgcg gtactcgctc
     5041 tggtaattcg cgttgtaaat agccagcagc tcatccgcca gttcagtcgc cagagtacga
     5101 gtgagtgctt cgcgtacttc ggcaatagca atcgggtcga tgatatcgaa cagttcagcc
     5161 atttcattga cagaaggcag cgtcaggatt tctgccgcca gcgccggatc aatcttctca
     5221 tcgagcagca ccgcgcggaa agcgtcagcc acatgtaccg gcagagacag cggctgccct
     5281 tgctgatgac gggcgacgtt cagcttgatg taggttgcca gcaaactttg cgccgcatcc
     5341 caacgggaga aatcattacg cgcatgacgc atcaggaagg tcagttgctg atcgctccac
     5401 ttatattcca gtttcactgg cgcagagaat tcgcacagca gcgcaggcac cggctggaag
     5461 tagacattat caaaaacaaa ggtctgttcc gcctgggtga cgttcagcac ggaattcacc
     5521 ggatgaccgc ctttctgcaa cgggatcact ttgccttcgt tatcatacag ttcgatggca
     5581 aacggaatat gcagcggctg tttttctgcc tgatccggag tggctggcgt gcgctggctg
     5641 atggtcaggg tgtactgctc ggtttccgga ttgtagtcgt ctttgacggt cacaaccggc
     5701 gtaccggact ggctgtacca acggcggaaa tgggagagat cgacattcga cgcatcttcc
     5761 atcgcctgca caaaatcgtc acaggtcgct gcgctaccat catgacgctc gaaatagagc
     5821 tgcatccctt tctggaaatt ttcttcgcca agcagggtgt ggatcatgcg aatcacctcc
     5881 gcgcccttct cgtaaacggt cagggtgtag aagttgttca tctcaatgac catatccggg
     5941 cggattgggt gcgccatcgg gctggcgtct tccgcaaact gcaatccgcg catggtgcgc
     6001 acattattga tacggtttac cgcgcgggaa ccaaggtcag agctgaattc ctgatcgcgg
     6061 aagacggtta aaccttcttt caggctgagc tggaaccagt cgcggcaggt cactcggtta
     6121 ccggtccagt tatggaaata ttcatggccg ataacgcgtt caatatcgag gtaatctttg
     6181 tcggtggcgg tatcggtgcg ggccagcaca tatttggagt taaagatatt cagcccctta
     6241 ttctccattg cgcccatatt gaagaaatcc accgcgacga tcatatagat gtcgaggtca
     6301 tactccaggc caaagcgttc ttcatcccat ttcatggagt ttttcagcga ggtcatcgcc
     6361 cacggcgcgc gatcaaggtt gccgcgatcg acgtacagct ccagtgctac ttcgcgacca
     6421 gaacgcgtgg taaaggtatc gcgcagtaca tcaaagtcgc ctgccaccag cgcaaacagg
     6481 tagcacggtt tcgggaacgg gtcctgccac tgtacccaat ggcgtccgtt ttccagttcg
     6541 ccttgcgcaa cgcggttacc attggaaagc aggaagggat atttgatttt atcggcaata
     6601 attttggtgg taaaacgcgc cagcacgtcc gggcggtcga gataatacgt aatatggcgg
     6661 aaaccttcgg cttcacactg ggtgcaaagc gcatcgcctg actgataaag cccttccagc
     6721 gccgtattcg ccgccgggct tatttcatta atgatcttaa gcgtaaaacg ctccggcaaa
     6781 ttactgatga ccagtgcgcc ctcttcttct ttacaggcgg tccacggctc atcattaata
     6841 tgaacagaaa ccagtttgag gtcttcgccg ttgagacgaa gcggagcatc tgatgcacca
     6901 tgacggacag cctggctgac cgcggtaacg accgtctttt gcgcgtcgag gtcaaaggtc
     6961 aagtcaatat cagtaatctg gtaatccggc gcacgataat cgtgacggta tttggcttgt
     7021 ggctgttgag tcataaataa cctttagcat cttttataga gtctggtgtt cagtctattc
     7081 ctgttgcgta aatcgcgcta tgcagaatct tcatcttttc aggtacaaac gcctttattg
     7141 ctacattttt ataacataca ccgcgtaatg ccatcgacca gaaaggtggc atatggtgtg
     7201 atcggggttc aataaattgc gaaacaaggt atactccagc agttcctgaa gatgtttatt
     7261 gtactaaacg ctcctgtacg aggacgctac tgcgcaccta tgacacaatt cgcttctcct
     7321 gttctgtact cgttgctgga tacagatgct tataagttgc atatgcagca agccgtgttt
     7381 catcactatt acgatgtgca tgtcgcggcg gagtttcgtt gccgaggtga cgatctgctg
     7441 ggtatttatg ccgatgctat tcgtgaacag gttcaggcga tgcagcacct gcgcctgcag
     7501 gatgatgaat atcagtggct ttctgccctg cctttcttta aggccgacta tcttaactgg
     7561 ctacgcgagt tccgctttaa cccggaacaa gtcaccgtgt ccaacgataa tggcaagctg
     7621 gatattcgtt taagcggccc gtggcgtgaa gtcatcctct gggaagttcc tttgctggcg
     7681 gttatcagtg aaatggtaca tcgctatcgc tcaccgcagg ccgacgttgc gcaagccctc
     7741 gacacgctgg aaagcaaatt agtcgacttc tcggcattaa ccgccggtct tgatatgtcg
     7801 cgcttccatc tgatggattt tggcacccgt cgccgttttt ctcgcgaagt acaagaaacc
     7861 atcgttaagc gtctgcaaca ggaatcctgg ttcgtgggca ccagcaacta cgatctggcg
     7921 cgtcggcttt ccctcacgcc gatgggaaca caggcacacg aatggttcca ggcgcatcag
     7981 caaatcagcc cggatctagc caacagccag cgagctgcac ttgctgcctg gctggaagag
     8041 tatcccgacc aacttggcat tgcattaacc gactgcatca ctatggatgc tttcctgcgt
     8101 gatttcggtg tcgagttcgc tagtcggtat cagggcctgc gtcatgactc tggcgacccg
     8161 gttgaatggg gtgaaaaagc cattgcacat tatgaaaagc tgggaattga tccacagagt
     8221 aaaacgctgg ttttctctga caatctggat ttacgcaaag cggttgagct ataccgccac
     8281 ttctcttccc gcgtgcaatt aagttttggt attgggactc gcctgacctg cgatatcccc
     8341 caggtaaaac ccctgaatat tgtgattaag ttggtagagt gtaacggtaa accggtggcg
     8401 aaactttctg acagccctgg caaaactatc tgccatgata aagcgtttgt tcgggcgctg
     8461 cgcaaagcgt tcgaccttcc gcatattaaa aaagccagtt aatatcatca gggagctaat
     8521 cggctccctt tttttacctt taattccgaa atctttcgct gcatttgcga attctgcttg
     8581 tctgattgca gatgccaggt aacataggta tcccccccat ttacgggtgg acaagtgttt
     8641 attttttccg actattaaca gagagaatat tatgagcgtt gtgcctgtag ccgacgtact
     8701 ccagggccgc gtagccgttg acagcgaagt caccgtacgc ggatgggtac gtacccgccg
     8761 agattcaaaa gctggcatct ccttcctcgc cgtttatgac ggttcctgct ttgatcctgt
     8821 acaggctgtc atcaataatt ctctgcccaa ttacaatgaa gacgtcctgc gtctgaccac
     8881 cggctgctcg gtcattgtga cgggtaaagt cgtggcgtcg ccgggccagg ggcaacaatt
     8941 tgaaattcag gccagcaagg ttgaagttgc tggttgggtt gaagatccag acacttaccc
     9001 gatggcggca aaacgccaca gcattgagta tctgcgtgaa gtcgctcacc tgcgtccgcg
     9061 cacaaacctg attggtgccg tcgcgcgcgt tcgtcatacg ctggcgcagg cgctgcatcg
     9121 cttctttaac gagcagggat tcttctgggt ttcaacgcca ctgattaccg catctgatac
     9181 cgaaggtgca ggcgaaatgt tccgcgtttc tacgctggat ctggaaaacc tgccgcgtaa
     9241 cgatcagggc aaagtggatt tcgacaaaga cttctttggt aaagagtctt tcctgaccgt
     9301 atctggccaa ttgaacggcg aaacctacgc ttgcgcattg tccaaaattt ataccttcgg
     9361 cccgactttc cgtgctgaaa actccaacac cagccgtcac ctggcggaat tctggatgct
     9421 ggagccggaa gtggcgtttg ctaacctgaa cgatatcgcg ggtctggctg aagccatgct
     9481 gaaatatgtc ttcaaagcgg ttctcgaaga acgcgcagac gacatgaaat tcttcgctga
     9541 acgcgtagat aaagatgccg tttcacgtct ggaacgcttc attgaagccg attttgcgca
     9601 ggtggattac accgacgcag tgaccattct cgaaaactgc ggcaggaagt ttgaaaaccc
     9661 ggtttactgg ggcgtcgatc tctcttctga gcatgagcgt tatctggcgg aagaacactt
     9721 taaagcaccg gtagtggtta aaaactaccc gaaagatatt aaagcgtttt atatgcgcct
     9781 taacgaagac ggtaaaaccg ttgcggctat ggacgttctg gctccgggca tcggtgagat
     9841 tattggtggt tcccagcgtg aagaacgtct ggacgtgctg gacgagcgta tgctggaaat
     9901 gggcctgaat aaagaagatt actggtggta tcgcgatctg cgtcgctacg gtactgttcc
     9961 gcattcaggt ttcggtcttg gttttgaacg cctgattgct tacgtaactg gcgtgcaaaa
    10021 cgtacgtgat gtgattccgt tcccacgtac tccgcgtaac gccagcttct aatttagcgt
    10081 ctacaagagc cagcacccgc tggctctttt ttatcctttc tgtcaagtta tctgtttgtt
    10141 aagtcaatca atctatttgc aaccccgcca taacgatcat cctgttacgg aatattacat
    10201 tgcaacattt acgcacaaaa aataacccgc attcttattg cggattagtt ttttcttagc
    10261 tgatagcaca attttcatac tattttttgg cattc
//
LOCUS       DAPGRN010000144        10223 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPGRN010000144 DAPGRN010000000
VERSION     DAPGRN010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.146"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            464..1441
                     /gene="csiD"
                     /locus_tag="RHW33_004082"
     CDS             464..1441
                     /gene="csiD"
                     /locus_tag="RHW33_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV1647536.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLTPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFHHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDIDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            1460..2728
                     /gene="lhgO"
                     /locus_tag="RHW33_004083"
     CDS             1460..2728
                     /gene="lhgO"
                     /locus_tag="RHW33_004083"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV1647537.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2751..4199
                     /gene="gabD"
                     /locus_tag="RHW33_004084"
     CDS             2751..4199
                     /gene="gabD"
                     /locus_tag="RHW33_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV1647538.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4213..5493
                     /gene="gabT"
                     /locus_tag="RHW33_004085"
     CDS             4213..5493
                     /gene="gabT"
                     /locus_tag="RHW33_004085"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV1647539.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIIGQCFAEAKQ"
     gene            5911..7311
                     /gene="gabP"
                     /locus_tag="RHW33_004086"
     CDS             5911..7311
                     /gene="gabP"
                     /locus_tag="RHW33_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV1647540.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7332..7994
                     /gene="csiR"
                     /locus_tag="RHW33_004087"
     CDS             7332..7994
                     /gene="csiR"
                     /locus_tag="RHW33_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV1647541.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRQTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7995..8444)
                     /gene="kbp"
                     /locus_tag="RHW33_004088"
     CDS             complement(7995..8444)
                     /gene="kbp"
                     /locus_tag="RHW33_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV1647542.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8528..8686)
                     /locus_tag="RHW33_004089"
     CDS             complement(8528..8686)
                     /locus_tag="RHW33_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV1647543.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8869..9168
                     /locus_tag="RHW33_004090"
     CDS             8869..9168
                     /locus_tag="RHW33_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1647544.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9178..9702
                     /gene="ygaP"
                     /locus_tag="RHW33_004091"
     CDS             9178..9702
                     /gene="ygaP"
                     /locus_tag="RHW33_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV1647545.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9749..10153)
                     /gene="stpA"
                     /locus_tag="RHW33_004092"
     CDS             complement(9749..10153)
                     /gene="stpA"
                     /locus_tag="RHW33_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV1647546.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2337 a         2638 c         2800 g         2448 t
ORIGIN      
        1 ccgaaaaatt atccctcctg gtaaagcatt tatgcttaga ggcggaactc taaatatcaa
       61 tattcctggg cgtagcgctc ttcttttagg gaaaacagga gaaccgctga actatctcta
      121 tttgtaattt aatgttatat ctgccccgct aaaacggggc agataatatg tttagtttac
      181 tagcggtcat tttgcagtga agccatttac cgttttttat cgaccagata atctgttctc
      241 tgatggtaat ccccccctaa cctgttgctt tagttattca tttcctgtct cactttgcct
      301 taatacccta cgttaaatgt taccaatttg ttgcttttga tcacaataag taaacaatat
      361 ggcgcatttg tgcgcatttt tcagaaatgt agatattttt agattatggc tacgaaatga
      421 gcatcgccat gtcaccctac atctcataag aggatcgctt ctgatgaatg cactgaccgc
      481 cgtacaaaac aacgctgtcg attcaggcca ggactatagc ggattcaccc tcaccccatc
      541 ggcgcaatcc ccgcgtctgc tggaactcac cttcaccgaa cagacgacca aacagtttct
      601 cgaacaggtt gccgagtggc ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg
      661 ggtaggcaaa attcttgacg atctgtgtgc gaatcagctg caaccattgc tgttgaagac
      721 cctgctaaac cgtgctgaag gtgcgttgtt gatcaatgcg gtgggtatcg atgatgtggc
      781 tcaggcagat gagatggtga agctggcaac ggcggtagcg catctgattg gccgttccaa
      841 ttttgatgcg atgagcggtc agtattacgc gcgttttgta gtgaaaaatg tcgataactc
      901 agacagctat ctgcgtcagc cgcaccgcgt aatggagctg cacaacgacg gcacctacgt
      961 cgaagagatc accgactatg tgctgatgat gaaaatcgac gagcagaaca tgcagggtgg
     1021 aaattcgttg ctgctgcatc tcgatgactg ggaacatctg gaccactatt tccaccaccc
     1081 actggcgcgc cgcccaatgc gctttgccgc gcctccgagc aaaaacgtca gcaaagatgt
     1141 tttccatccg gtgttcgaca ttgatcagca gggccgcccg gtgatgcgct atatcgacca
     1201 gttcgtccag ccgaaagact tcgaagaagg cgtgtggttg agcgagcttt cggacgccat
     1261 tgaaaccagt aaaggcattc tttctgtccc cgttcccgtt ggcaaattcc tgttgattaa
     1321 caacctgttc tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga
     1381 actgatgcgt cagcgtggct atttcgctta cgcctctaac cactaccaga cgcatcagta
     1441 aggcaaagga attgagcgga tgtatgattt tgtgattatt ggcggcggca tcatcggcat
     1501 gtcgaccgcc atgcaactga ttgatgtcta tccggacgca cgcattgcgt tgctggaaaa
     1561 agagtccggc ccggcctgtc accaaacggg ccacaacagt ggcgtgatcc atgccggggt
     1621 ttattacacg cccggtagcc tgaaggcaca gttttgcctg gcgggaaacc gcgccaccaa
     1681 agccttttgc gatcaaaacg gcattcgcta cgacaactgc ggcaagatgc tggtcgccac
     1741 gtctgaactc gaaatggaac ggatgcgcgc cttatgggaa cgcacggcgg caaacggtat
     1801 cgagcgcgag tggttaaacg ccgatgaact gcgcgagcgc gaaccgaata tcaccgggct
     1861 tggcggtatt tttgtgccgt ccagcggcat tgtcagctac cgcgaagtaa cggcggcgat
     1921 ggcaaaaatt ttccaggcca gaggcggcga gattatctat aacgccgaag tcagcggcct
     1981 tagtgagcat aaaagcggcg tggtgatacg tacccgtcag ggcggcgact atgaagcatc
     2041 gacgctgatt agctgttccg ggctgatggc tgaccggctg gtgaaaatgc tcggcctcga
     2101 accgggcttt attatctgcc cgttccgcgg cgagtatttc cgcctggcgc cggagcataa
     2161 ccagattgtt aaccatctga tttaccccat tcccgacccg gcaatgccgt ttttgggcgt
     2221 gcatctcacc cgcatgatcg acggcagcgt caccgtcggg ccaaacgcgg tgctggcttt
     2281 caaacgcgaa ggctatcgca agcgcgactt ctcatttagc gacacgctgg agattttggg
     2341 ctcgtcgggg attcgccggg tgctgcaaaa ccatctacgc tcaggactgg gcgagatgaa
     2401 aaactcgctg tgcaaaagcg gctatctgcg gctggtgcaa aagtattgtc cccggctttc
     2461 gttaagcgat ctccagccct ggcccgccgg tgtgcgggcg caggcggtat cgccggacgg
     2521 caagctgatt gacgattttc tgtttgtcac caccccgcgc acgatccaca cctgcaatgc
     2581 gccctccccg gcagcgacat cagcaattcc tattggtgcg catattgtca gcaaggtaca
     2641 aacgctgttg gcaagccaga gtaaccccgg acgcacgctg cgagcggcac gtagtgtgga
     2701 tgccttacac gccgcattta atcaataacc tttgaaaaca ggatgtagcg atgaaactga
     2761 acgacagtaa cttattccgc cagcaggcgt tgattaacgg ggaatggctg gacgccaaca
     2821 atggtgaagt catcgacgtc accaatccgg cgaacggcga caagctgggt agcgtgccga
     2881 aaatgggcgc ggatgaaacc cgcgccgcta tcgacgccgc caaccgcgcc ctgcccgcct
     2941 ggcgtgcgct caccgccaaa gaacgcgcca acattctgcg caactggttc aatttgatga
     3001 tggagcatca ggacgattta gcgcgcctga tgaccctcga acagggcaaa ccactggctg
     3061 aagcgaaagg tgaaatcagc tacgccgcct cttttattga gtggtttgcc gaagaaggca
     3121 aacgtattta tggcgacacc attcccggtc accaggccga taaacgcctg attgttatca
     3181 agcagccgat tggcgtcacc gcggctatca cgccgtggaa cttcccggcg gcgatgatta
     3241 cccgcaaagc cggtccggcg ctggcggcag gctgcaccat ggtgctgaag cccgccagtc
     3301 agacgccgtt ctctgcgctg gcgctggcgg agctggcgat ccgcgcgggc attccggctg
     3361 gcgtgtttaa cgtggtcacc ggttcggcgg gcgcggtggg taatgaactg accagcaacc
     3421 cgctggtacg caaactgtcg tttaccggct cgaccgaaat tggccgccag ttaatggaac
     3481 agtgcgcgaa agacatcaaa aaagtgtcgc tggagctggg cggcaacgcg ccgtttatcg
     3541 tctttgacga tgccgacctc gacaaagccg tggaaggcgc gctggcctcg aaattccgca
     3601 acgctgggca aacctgcgtc tgcgccaacc gtttatacgt gcaggacggc gtgtatgacc
     3661 gttttgccga aaaattgcag caggcggtga gcaaactgca catcggcgac gggctggaga
     3721 aaggcgtcac tattgggccg ctgattgatg aaaaagcggt agcaaaagtg gaagagcata
     3781 tcgccgatgc gctggagaaa ggcgcgcgcg tggtttgcgg cggtaaagca catgaacgcg
     3841 gcggcaactt cttccagccg accattctgg tggacgttcc ggccaacgcc aaagtgtcga
     3901 aagaagagac gttcggcccc ctcgccccgc tattccgctt taaagatgaa gccgatgtga
     3961 ttgcgcaagc caatgacacc gaatttggtc ttgccgccta tttctacgcc cgtgatttaa
     4021 gccgcgtctt ccgcgtgggc gaagcgctgg agtacggcat cgtcggcatc aataccggga
     4081 ttatttccaa tgaagtggcc ccgttcggcg gcattaaagc ctcgggtctg ggtcgtgaag
     4141 gttcgaagta tggcatcgaa gattacttag aaatcaaata tatgtgcatc ggtctttaac
     4201 tggagaacgc gaatgagcag caataaagag ttaatgcagc gccgcagtca ggcgattccc
     4261 cgtggcgttg ggcaaattca cccgattttc gctgaccgcg cggaaaactg ccgggtgtgg
     4321 gacgttgaag gccgtgagta tcttgatttc gcgggcggca ttgcggtgct caataccggg
     4381 cacctgcatc cgaaagtggt tgcggcggtg gaagcgcagt tgaaaaaatt gtcgcacacc
     4441 tgcttccagg tgctggctta cgagccgtat ctggagctgt gcgagattat gaatcagaag
     4501 gtgccaggcg atttcgccaa gaaaacgctg ctggttacga ccggttccga agcggtggaa
     4561 aacgcggtga aaatcgcccg cgctgccacc aaacgtagcg gcaccatcgc ttttagcggc
     4621 gcgtatcacg ggcgcacgca ttacacgctg gcgctgaccg gcaaggtgaa tccgtactct
     4681 gcgggcatgg gcctgatgcc agggcacgtt tatcgcgcgc tttatccttg cccactgcac
     4741 ggcatcagtg aagatgatgc tatcgccagt atccaccgaa tttttaaaaa tgatgcggcg
     4801 ccggaagata ttgccgccat cgtgattgag ccggttcagg gcgaaggcgg tttctacgcc
     4861 gcgacgccag cttttatgca gcgtttacgc gcgctgtgtg atgagcacgg gatcatgctg
     4921 attgccgatg aagtgcagag cggcgcgggg cgtaccggca cgctgtttgc gatggagcaa
     4981 atgggcgtgg caccagatct taccaccttt gcgaaatcga tcgcaggcgg cttcccgctg
     5041 gcgggcgtca ccgggcgcgc ggaagtgatg gatgccgtcg ctccaggcgg tctgggcggc
     5101 acctatgcgg gtaacccgat tgcctgcgtg gctgcgctgg aagtgttgaa ggtgtttgag
     5161 caggaaaatc tgctgcaaaa agccaacgat ctggggcaga agttgaaaga cggattgctg
     5221 gcgatagccg aaaaacaccc ggagatcggc gacgtacgcg ggctgggagc gatgatcgcc
     5281 atcgagctgt ttgaagatgg cgatcacagc aaaccggacg ccaaactcac cgccgaaatc
     5341 gtggcgcgcg cccgcgataa aggtctgatt cttctctcct gcggcccgta ttacaacgtg
     5401 ctgcgcatcc ttgtaccgct cacaattgaa gatgctcaga ttcgtcaggg tctggagatc
     5461 atcggccagt gttttgctga ggcgaagcag tagcaccgcc cccatgccgg aggcgacgct
     5521 tacgcgtctt gtccggccta cgagagtgta ggtcggataa ggcgttaacg ccgcatccga
     5581 caatcggcgc accatgccgg aggcaacgct tacgcgtctt gtccggccta cggggattca
     5641 ggtcggataa ggcgtttacg ccgcatccga cattcggcgc accatgccgg aggcgacgct
     5701 ttgcgtcttg tccggcctac ggggattctc gtcggataag gcgtttacgc cgcatccgac
     5761 aatctgtacg tgaacaggaa gaaatctatg ttggccgggt aaggcggagc cgctctccgg
     5821 caaaaagaac caataacaat taaacgcgtg acccgacgcg ggaaatgtcg gggcgctctc
     5881 ccaagtgaca cactttcgag aggattcagg atggggcaat catcgcaacc acatgagtta
     5941 ggcggcgggc tgaagtcacg ccacgtcacc atgttgtcta ttgccggtgt tatcggcgca
     6001 agtctgtttg tcggttcaag cgtcgccatc gccgaagcgg gcccggcggt attactggcc
     6061 tatctgttcg ccggattact ggtggttatg attatgcgga tgctggcgga aatggcggtt
     6121 gccacgcccg ataccggttc gttttccacc tatgccgata aagccattgg ccgctgggcg
     6181 ggctatacca tcggctggct gtactggtgg ttttgggtac tggttatccc actggaagcc
     6241 aacatcgccg ccatgatcct gcactcgtgg gttccaggca ttcccatctg gttattttcc
     6301 ctcgtcatta ccctcgcctt aaccggcagt aatttattaa gcgttaaaaa ctacggcgaa
     6361 tttgagttct ggctggcgct gtgcaaagtc atcgctatcc tggcctttat tttccttggt
     6421 gcagtcgcaa ttagcggttt ttacccgtat gccgacgtga gcggtatttc aagattgtgg
     6481 gatagcggcg gctttatgcc caacggcttc ggcgcggtat taagcgcgat gttgatcacc
     6541 atgttctcgt ttatgggcgc agaaattgtc accattgccg ccgcggaatc cgacacgccg
     6601 gaaaaacata ttgtccgcgc caccaactcg gttatctggc gtatttctat cttctattta
     6661 tgctctattt ttgtcgtcgt ggcattaata ccgtggaata tgccggggct gaaagccgtt
     6721 gggtcttatc gctcggttct ggaattgctc aatattcccc atgcgaaatt aatcatggac
     6781 tgcgtgatat tactttccgt aaccagttgc ctgaactcgg cgctgtatac cgcttccagg
     6841 atgctctact ccttaagccg tcgcggtgat gcgcccgcgg taatgggtaa aatcaaccgc
     6901 agtaaaaccc cgtacgtggc ggtgttactc tccaccggcg cagcattttt aacggtggtg
     6961 gtgaactatt acgcacctgc gaaagtgttt aaatttctga tcgacagctc cggtgctatc
     7021 gccctgctgg tttatttagt catcgccgtt tcacagttgc ggatgcgtaa aattctgcga
     7081 gcagaaggaa gcgaaattcg cttgcgcatg tggctttacc cgtggctcac ctggctggta
     7141 atcggcttta ttacctttgt gttggtggtg atgttattcc gcccggcgca acagttagaa
     7201 gtgatctcta ccggcttatt agcgataggg attatctgta ccgtgccgat tatggcgcgc
     7261 tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata atacgcgctg ataatgatca
     7321 ggagtcacac catgaccatt acgtctctgg atggctatcg ctggctgaag aacgatatta
     7381 ttcgcggtaa ttttcaaccg gatgaaaaat tacgaatgag tttgctgaca tcgcgttatg
     7441 cacttggcgt tgggccgtta cgggaagctc tttcgcaact ggtggcggaa cggctggtca
     7501 cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag ctgctcgata
     7561 ttttcgatgc ccgcgccaat atggaagcga tgttagtgag tctggcgatt gcccgcggtg
     7621 gcgatgagtg ggaggcagac gttctcgcaa aagcgcatct gctgagtaag cttgaggcct
     7681 gtgacgccag cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg tttcatacgg
     7741 caattgtggc aggctgtggt tctcactatt tgctgcaaat gcgtgaacgg ttgtttgatc
     7801 tggcggcgcg ctatcgattt atctggctgc gacaaacagt gctttcggtg gaaatgctgg
     7861 aggataaaca cgatcagcac cagaccctga ctgcggcggt actggcgcga gataccgcgc
     7921 gcgccagtga gttaatgcgc cagcatttac tgacgccaat tcccattatc cagcaggcga
     7981 tggctggcaa ttaactactc ttccggaata cgcaacactt gccccggata aattttatcc
     8041 gggcttttta gcatcggttt attcgcttcg aagattttat tgtacagatt agcgttaccg
     8101 tagacctgtt tggaaatggc actcagagtg tcgccagact taacggtata aaactggctg
     8161 gcagtggctg gtgtcgccgt tttcacctga tcatcaacac tggcaatgcc ggaaatattc
     8221 cccaccgcaa caaggatttt ctctttcgcc tcctgactca gaccgtcacc agtgaccgtc
     8281 gctttgccgt cggcaatttg aatgttcact ttatcggcat caggtatacc ggttttgctc
     8341 agatgctcct gcaccttctt cgcctgatcg tctttatcgt gctgacctgt aaccgcgtcc
     8401 cagagttttt ctccggcatc tttcacaaaa ttgaacagac ccatagctac ctcattcgtt
     8461 aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc gttaacgccg cgggggtgag
     8521 cacaggttta atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg atatacccaa
     8581 gcagtgtcag cagaatatta ataatgaacg cccaaccgaa ccctttaccg agcagcacgc
     8641 cgagcggcgg cagaatgatg gtgatgacga ttctccagaa acccatatgt actccctata
     8701 agaaaattac tcattgttta aaaagagatt ttatctctta agcgtagtaa ttttagcgga
     8761 ggctgccagt ttcagcttac gcttttactc tcatttacgc tgctgaggct ggtatatgtt
     8821 gcatttttag tttagagttt acttaattta ggaaatgctt aaataaatat gactgaactc
     8881 gcgcaattac aggccagtgc cgaacaggca gcggccttat tgaaagcaat gagccaccct
     8941 aaacggttgc tgattctgtg catgcttagc ggttcccccg gcaccagcgc gggagagctg
     9001 acgcgcatta ccggactgag tgcctctgcg acatcacagc atctcgcccg tatgcgggac
     9061 gaagggctta tcgacagcca acgggatgcc caacgcattc tatattccat taaaaatgag
     9121 gcggtaaatg ccattatcgc caccctgaaa aatgtctatt gtccgtaagg agtcactatg
     9181 gctttgacaa ccatttcgcc gcatgatgca caagaattaa tcgcacgcgg cgcaaagtta
     9241 atcgatattc gtgacgctga tgaatatctt cgtgaacata ttcctgaagc agatctggct
     9301 ccattatccg tgctggaaca gtcaggtctc ccggctaaat tacgtcgcga gcaaattata
     9361 ttccactgcc aggcaggtaa acgcaccagt aataatgccg ataaattagc agcgattgcc
     9421 gcccccgcag aaatcttttt actcgaagat gggattgatg gctggaaaaa agcaggattg
     9481 ccagtagcgg taaataaatc tcaacccttg ccgttaatgc gccaggtgca gatcgctgcg
     9541 ggtggtttaa tattaatcgg cgttgtactg ggttataccg taaatagcgg tttcttctta
     9601 ttaagtggct ttgttggtgc cgggttactg tttgcaggaa tcagcggttt ttgtggaatg
     9661 gcaaggttgt tagataagat gccgtggaac caacgagctt gagaagcgac gccggacgcg
     9721 ccctggcagc gacatccggc ctcagtaatt agatcaggaa atcgtcgaga gatttacctt
     9781 ctgccagcgc ctgagcaatt ggcttcggtg tacggccctg accggtccag gttttagttt
     9841 caccgttaac atcggtgaat ttatatttcg ccggacgcgg ctggcgtttt ttaccagcgc
     9901 gtggtgcagc agcagagcta ttacccaata actcttccgg gttaattccg tcagctttca
     9961 tcagctccag ccaggtgcta attttttcct ggcgctctgc cagttcacgc tgctgctgtt
    10021 cttcttcttc acgtctttct ttagtgacaa ccctgaattt ttcgagcatt tcttcaagaa
    10081 cgtcaatgga gaattcgcga gccatcgcac ggagggtgcg aatgttattt aaactttgta
    10141 acattacgga cataataaag aaaacctttt aacgccaaaa caaaaaagta tttcgtcgcg
    10201 tagtgtaagc gatcacttat tat
//
LOCUS       DAPGRN010000145        10105 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPGRN010000145 DAPGRN010000000
VERSION     DAPGRN010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.147"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..886
                     /locus_tag="RHW33_004093"
                     /pseudo
     CDS             1..886
                     /locus_tag="RHW33_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            899..1813
                     /locus_tag="RHW33_004094"
     CDS             899..1813
                     /locus_tag="RHW33_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1647547.1"
                     /translation="MLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFDLRFLSAIKQ
                     KVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLTCEAHSHRSVALAKTVAAPIMATG
                     VGAALMYATSSGPYYAQSLSGSPDIEMIKNRMAKLFNQSDTSVRNKYRPAFDKWNELV
                     GKLNHERNTVPFACLTTILATAINETPEGDTNVAVVMGCKSAKDRTISIVLGNSMLQT
                     LFEKRLADDGEIENLFDQQGYFSCDSLTAEEVMMLKDLFDIRVLHLSNKFNVGLQGNI
                     NTDVLQDSFFKNVDFIHESNSYTVGMGV"
     gene            2488..2706
                     /locus_tag="RHW33_004095"
     CDS             2488..2706
                     /locus_tag="RHW33_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV1647548.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2923..3164
                     /locus_tag="RHW33_004096"
                     /pseudo
     CDS             2923..3164
                     /locus_tag="RHW33_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312843.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3494..4423)
                     /gene="fdhE"
                     /locus_tag="RHW33_004097"
     CDS             complement(3494..4423)
                     /gene="fdhE"
                     /locus_tag="RHW33_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV1647549.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4420..5055)
                     /gene="fdoI"
                     /locus_tag="RHW33_004098"
     CDS             complement(4420..5055)
                     /gene="fdoI"
                     /locus_tag="RHW33_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV1647550.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5052..5954)
                     /gene="fdoH"
                     /locus_tag="RHW33_004099"
     CDS             complement(5052..5954)
                     /gene="fdoH"
                     /locus_tag="RHW33_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV1647551.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5967..9017)
                     /gene="fdnG"
                     /locus_tag="RHW33_004100"
     CDS             complement(5967..9017)
                     /gene="fdnG"
                     /locus_tag="RHW33_004100"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8430..8432),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV1647552.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9211..10044
                     /gene="fdhD"
                     /locus_tag="RHW33_004101"
     CDS             9211..10044
                     /gene="fdhD"
                     /locus_tag="RHW33_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV1647553.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2551 a         2423 c         2573 g         2558 t
ORIGIN      
        1 ggttgtcaat attaatctca ttaaacaaga aagttattct gttgttaatt tagaaaaaca
       61 actaatgaga aataaatcac cgaagcaaac gctattaaat gatttttcac atcgccttaa
      121 aatggaaggt gaggaacaac tgttccattg tgttaacgaa ctgaagtatg gtttgtcgag
      181 taataatgaa tatgacctgt ttaaaaatag agaaacctca ctgtggaaac gcaaagtacc
      241 agaaagcgca gcgctggcaa tgacgggaac agtatataaa ttatttggta gtcgcttact
      301 caacgcccgc aatcagttgg ataaaaatct cctcgaaatg gctatgcaaa tggtttatgg
      361 caaagatatg gtgacaaagt ccggggatat attgattgat atacaattaa ataaggatgg
      421 cagcctccag agtaagcact acacatttga tgtaggtgaa gttataaaca gttataatat
      481 tgacctcttt gtgaatgctt cgagtattaa tcaaacttac ttacatgata aaaatcctgg
      541 tgacgttatt agcctgtatg gcactttaga taacattccc cttgtagtta aggaatgcac
      601 gggtaaaaat catcaaatta acgttaaagc caaattgcca caagaacgaa gtgaaaaagt
      661 tgaggatatg tgtgaaagaa tgagaggtgg catctctatg tttaatcaca ccacaaaaac
      721 agctggtaat atagagcata atttgcagct aagttttctc gtggattcac gaccgtcgat
      781 caccaataaa tactcagcca aagatgtagc gccagacata cttgtattgc ccgtcaacgt
      841 atatcatgcg gattttaaaa ttaaaatcaa tgacgcagta gagaaggctt cgtcaattgt
      901 gttatgtgct gtgcaaaaaa atctccatgc agcaagaaag gttctcagcg ggataatagg
      961 aaatgtgcca aaaaataata aaccaaaaac aatcattttt gatttacgtt ttcttagtgc
     1021 aattaagcag aaagtttttt tgggcagtga aaaaccgttg tttgtaaagc atacggcaat
     1081 gatgagggag gcatgtaagg aattaccgca gtcagtggaa tatgttcctt taacctgtga
     1141 agcgcatagc catcgcagcg tcgcccttgc caaaaccgtg gctgcgccca tcatggcgac
     1201 aggtgtgggc gcggctttaa tgtatgcaac atcatcagga ccgtattatg cgcaaagtct
     1261 ttctggttcg ccggatatcg agatgataaa aaatcgcatg gctaaattgt ttaaccagtc
     1321 ggatacttct gtgcgcaaca aatatcgccc ggcttttgat aaatggaatg aactggtagg
     1381 taaattaaat cacgaaagaa atacagttcc gtttgcgtgt ttgaccacca ttttagcgac
     1441 agcgataaat gaaactcctg agggggatac gaatgttgcc gttgtgatgg ggtgtaaatc
     1501 tgcgaaagat cgcacgatat ccattgtcct tggcaactcg atgttgcaaa cactctttga
     1561 aaaacgcctc gccgatgatg gagaaataga aaaccttttt gatcaacagg gatattttag
     1621 ttgcgattca ttaacagccg aagaagtaat gatgttaaaa gacctttttg atattcgagt
     1681 tcttcatctc tctaacaaat ttaatgttgg gttgcaaggt aatatcaaca cggatgtttt
     1741 acaagattcg ttttttaaaa atgtcgattt cattcatgaa tctaactcgt ataccgtagg
     1801 gatgggagtc tgaagagtaa aactcattag gattatcaga attcggccaa tgcattgcaa
     1861 aaatgaaata caaagagaga tgtgtgcagg cgcttagccg cgaggggcat ggcgaagcca
     1921 tacagaaagc accagcattc agaactcctg tgaaagcggc atagtgccgc tgctcaggag
     1981 ttttgttatg acccgtatcg tgctgagcgt tgccacccta tgatgcgatt aaaaaaactc
     2041 accgtcgagc cgatgattga cgtcaatgct atcggatgat gccgggggtg ttccggaagg
     2101 aattccttgt ttcccgaact gttaccaaca aaattataac gatgagcaag taaagaccgg
     2161 ttggtggcag tactgaccgg tctgtgatgg aagattaatt tcaccttaaa tcctggtgct
     2221 gttaggatgc tgacgttttt cggctacacc attcagatta tgcaaataaa tatccctgaa
     2281 tataatatat tcatagcacc cataactcat cctgggcgat aactaaacca tttttgagta
     2341 ataaatgaaa caactgcaac gctcttatta tttctggaaa acgtctcgca aaaacaaaac
     2401 aacatgcaaa aatccgcttt cgttaattaa ttttccggcg taattttcgc acatacatta
     2461 atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct tcatttatca
     2521 gaagaggcaa ttaaacggct gaacaagctt cgtggctggc gtaaggtttc acgatctgca
     2581 attttacgcg aagcggtaga gcaatatctg gaacgtcagc aattcccggt gcgtaaagca
     2641 aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa ggagcataag
     2701 gaatgacaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt ctggtattaa
     2761 tacaaggctg ttttacttga acttataata actgcaactg ttacatcata tctggaaaac
     2821 gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc gataagggta
     2881 cttttctctc catagatatc aaatgatatt ggtcaaatat ttatgaactc tctggcaggt
     2941 attgatatgg gcagaattat actcgattta tcgaatgagg tgattaagca acttgatgat
     3001 cttgaggtgc agcgtaatct tcctcgtacg gagctattac gggaagcggt agatcaatac
     3061 ttggtaaatc aatcgcaaca gtaagaacca gtgcttttgg catatggcaa gggcgtgagg
     3121 aagatggtgt cgaataccag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatat
     3181 caatcagatt gctgacaacg tgtgcgttgt tcatgccgga tgcggcgtga acgccttacc
     3241 cggcctacaa agtcgtataa attcaatgta ttgcagttta ttggtaggcc taataagcgc
     3301 ggcgcatcag gcaatcttac gcttatcatc agtctcatac ccataacctt attgatgtgg
     3361 gggtgggtaa attgaggcaa aacaggcact ggcggcatag cttgaggtgg taacgcggaa
     3421 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
     3481 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     3541 catagccttc ttgctccatt cgtgcgtcca gtaccagaga ggcgaggtca tctgccacgg
     3601 cttcaacttt cggttctttt tcctgataga gaattttcag gtaagtgccg cagtcatcgc
     3661 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     3721 tctgctcaca gttgctgcat tttacgcgca ctacgtgcca ttcagtttca cacaggttgc
     3781 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     3841 agccacatac cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga
     3901 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     3961 cgctactgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     4021 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     4081 tcagctcagc aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     4141 gaatatccag cgggggcttg ccttgtgcgc tggcttcttt aatgcgtgca gtcagatcca
     4201 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     4261 gcaggtaatc acccagcgga ttattttctg ccaactcgcg cagacgctcg gcgcggcggt
     4321 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     4381 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     4441 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     4501 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     4561 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     4621 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     4681 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     4741 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     4801 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     4861 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     4921 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat
     4981 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     5041 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     5101 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     5161 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     5221 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     5281 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     5341 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     5401 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     5461 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     5521 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     5581 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     5641 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     5701 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     5761 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     5821 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     5881 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc
     5941 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     6001 aactccggcg tctgcgtgtt tgcatcacca acgaatggcg ttaacgtatt ggcaataaag
     6061 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     6121 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     6181 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     6241 gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg
     6301 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     6361 gctttaccca atgcttcggc atcgtcttta aagatacgtg cagccgggtt cgagataacg
     6421 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     6481 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     6541 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     6601 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     6661 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     6721 agcgtgttac cgaggccaga cggatcggcg ttatcacggc gtgccatctg gttgccttcc
     6781 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     6841 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     6901 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     6961 tgcggaatgg cgtagttcca ggtcatgttc agcacttggt ccgggttcgc gccaccctgt
     7021 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc gtcagtcagc
     7081 gcaatccccg gggcgtccgc acctttccag tgccactgta accagcgacc tgagttaacg
     7141 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     7201 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     7261 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     7321 ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc
     7381 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     7441 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     7501 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     7561 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     7621 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     7681 cgcagggcgt taacaccgcc gccagccatc cccatgttac cgagcagcag ctggatcatc
     7741 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     7801 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     7861 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     7921 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     7981 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtcg
     8041 tagccggtga acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     8101 gtataggctt cagtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     8161 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc agctaccgcc
     8221 gccgtacgcg taaagcgagg atcgatcaca atcagcttag cgccgttgtg aattttggct
     8281 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     8341 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     8401 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     8461 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     8521 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc attttgcgca
     8581 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     8641 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     8701 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     8761 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     8821 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     8881 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     8941 aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     9001 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     9061 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     9121 cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     9181 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcaaaa agaaatcgaa
     9241 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     9301 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     9361 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     9421 ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     9481 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     9541 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     9601 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     9661 gatgatgcat tacgtcatct caacgatttt cagccagtgg gacaactgac tggttgtact
     9721 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt
     9781 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     9841 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     9901 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     9961 gagcgctgta atttgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
    10021 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
    10081 acttattcat ttcgtgaatt ttatt
//
LOCUS       DAPGRN010000146         9955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPGRN010000146 DAPGRN010000000
VERSION     DAPGRN010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.148"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            122..337
                     /gene="bdm"
                     /locus_tag="RHW33_004102"
     CDS             122..337
                     /gene="bdm"
                     /locus_tag="RHW33_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV1647554.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            439..576
                     /gene="sra"
                     /locus_tag="RHW33_004103"
     CDS             439..576
                     /gene="sra"
                     /locus_tag="RHW33_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV1647555.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            733..2430
                     /gene="maeA"
                     /locus_tag="RHW33_004104"
     CDS             733..2430
                     /gene="maeA"
                     /locus_tag="RHW33_004104"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV1647556.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2564..3574
                     /gene="adhP"
                     /locus_tag="RHW33_004105"
     CDS             2564..3574
                     /gene="adhP"
                     /locus_tag="RHW33_004105"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV1647557.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSHTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3721..4005
                     /locus_tag="RHW33_004106"
     CDS             3721..4005
                     /locus_tag="RHW33_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV1647558.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4234..4887)
                     /gene="fdnI"
                     /locus_tag="RHW33_004107"
     CDS             complement(4234..4887)
                     /gene="fdnI"
                     /locus_tag="RHW33_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV1647559.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4880..5764)
                     /gene="fdnH"
                     /locus_tag="RHW33_004108"
     CDS             complement(4880..5764)
                     /gene="fdnH"
                     /locus_tag="RHW33_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV1647560.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5777..8824)
                     /gene="fdnG"
                     /locus_tag="RHW33_004109"
     CDS             complement(5777..8824)
                     /gene="fdnG"
                     /locus_tag="RHW33_004109"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8237..8239),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV1647561.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKT"
     gene            9056..9937
                     /gene="yddG"
                     /locus_tag="RHW33_004110"
     CDS             9056..9937
                     /gene="yddG"
                     /locus_tag="RHW33_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV1647562.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2417 a         2614 c         2520 g         2404 t
ORIGIN      
        1 aaaaatcatc aaccaggact aatcttaaca acgaagcggc aaatatttgc catgctgaat
       61 gtgctttata aatctgcgat ccgtagcaga caccataaat acacagacac ggagaatcac
      121 tatgtttact tattatcagg cagaaaattc aacagcagaa cccgctctgg ttaatgccat
      181 tgagcaaggc cttcgggcag agcacggtgt tgtcactgaa gatgacattc tcatggagct
      241 gaccaaatgg gtggaagctt ctgataacga catcctcagt gatatctacc agcaaacgat
      301 caattatgtg gtcagtggcc agcaccctac gctttaaggt gctatgcttg atcggcaacc
      361 taatttaggg gtttagcacg tgtttcttcg ctacggcgat gttgtcctta aaactagcaa
      421 caggattgag gagttaaaat gaaatcgaac cgtcaggcac gtcatattct tggactggac
      481 cataaaatat ctaaccagcg caaaatagtt accgaaggtg acaaatccag cgtggtaaat
      541 aacccaaccg gcagaaaacg ccccgctgaa aagtaattca taaccatcag tcctcaatga
      601 cgattaaaca ccattgcctg cgcaatggtg tttttgtttt tatctgcttt atacttgggg
      661 ccgacgccct ggcggtaaag caaagacgat aaaagcgtgc cagggatgga tattcaaaaa
      721 agagtgagtg acatggaacc aaaaacaaaa aaacagcgtt cgctttatat cccttacgct
      781 ggccctgtac tgctggaatt tccgttgttg aataaaggca gtgccttcag catggaagaa
      841 cgccgtaact tcaacctgct ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa
      901 gcggaacgag catggatcca gtatcaggga ttcaaaaccg aaatcgacaa acacatctac
      961 ctgcgtaaca tccaggacac caacgaaacc ctcttctacc gtctggtaaa caatcatctt
     1021 gatgagatga tgcctgttat ttatacccca accgtcggcg cagcctgtga gcgtttttct
     1081 gagatctacc gccgttcacg cggcgtgttt atctcttacc agaaccggca caatatggac
     1141 gatattctgc aaaacgtgcc gaaccataat attaaagtga ttgtggtgac tgacggtgaa
     1201 cgtattctgg ggcttggtga ccagggcatc ggcgggatgg gcattccgat cggtaaactg
     1261 tcgctctata ccgcctgtgg cggcatcagc ccggcgtata cccttccggt ggtgctggat
     1321 gtcggcacca acaaccaaca gctgcttaac gatccgctgt atatgggctg gcgtaatccg
     1381 cgtatcactg acgacgaata ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa
     1441 cgctggccgg acgtgctgtt gcagtttgaa gacttcgcac aaaaaaatgc gatgccgtta
     1501 cttaaccgct atcgcaatga aatttgttcc tttaacgatg acattcaggg cactgcggcg
     1561 gtaacagtcg gcaccctgat cgcagcaagc cgcgcggcag gtggtcagtt aagcgagaaa
     1621 aaaatcgtct tccttggcgc aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc
     1681 cagacccagc gcgaaggatt aagcgaggaa gcggcgcggc agaaagtctt tatggtcgat
     1741 cgctttggct tgctgactga caagatgccg aacctgctgc ctttccagac caaactggtg
     1801 cagaagcgcg aaaacctcag tgactgggat accgacagcg atgtgctgtc actgctggat
     1861 gtggtgcgca atgtaaaacc agatattctg attggcgtct caggacagac cgggctgttt
     1921 acggaagaga tcatccgtga gatgcataaa cactgtccgc gtccgatcgt gatgccgctg
     1981 tctaacccga cgtcacgcgt ggaagccaca ccgcaggaca ttatcgcctg gactgaaggt
     2041 aacgcgctgg tcgccacggg cagtccgttt aatccagtgg tatggaaaga taaaatctac
     2101 cctatcgccc agtgtaacaa cgcctttatt ttcccgggca tcgggttggg tgttattgct
     2161 tccggcgcgt cacgtatcac cgatgagatg ctgatgtcgg caagtgaaac gcttgctcag
     2221 tattcgccgc tggtcctgaa cggcgaaggt ctggtactac cggaactgaa agatattcag
     2281 aaagtctccc gcgcaattgc gtttgcggtt ggcaaaatgg cacagcagca aggcgtggcg
     2341 gtgaaaacgt ctgccgaagc cctgcaacag gccattgacg ataatttctg gcaagccgaa
     2401 taccgcgact accgccgtac ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc
     2461 aatttccctc tctctttttc agatctcatc catactgggt agtggcgaat aaatctcatt
     2521 tgcctcacct gctatgcaga acatcatccg aaaaggagga actatgaagg ctgcagttgt
     2581 tacgaaggat catcatgttg acgttacgga taaaacactg cgctcactga aacatggcga
     2641 agccctgctg aaaatggagt gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg
     2701 cgattttggt gacaaaaccg gcgtaattct gggccatgaa ggcatcggtg tggtggcaga
     2761 agttggccca ggtgtcacct cattaaaacc aggcgatcgt gccagcgtgg cgtggtttta
     2821 cgaaggatgc ggtcattgcg aatactgtaa cagtggtaac gaaacgctct gccgttcagt
     2881 taaaaatgcc ggatacagcg ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga
     2941 ttacgcggta aaagtgccag atggtctgga ctcggcggcg gccagcagca ttacctgtgc
     3001 gggggtcacc acctacaaag ccgttaaact gtcaaaaatt cgtccagggc agtggattgc
     3061 tatctatggt cttggcggtc tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa
     3121 cgcgaaagtg atcgccattg atgtcaatga tgagcagtta aaactggcaa ccgaaatggg
     3181 cgcagattta gcgattaact cacacaccga agacgccgcc aaaattgtgc aggagaaaac
     3241 tggtggcgct cacgctgcgg tggtaacagc ggtagctaaa gctgcgttta actcggcagt
     3301 tgatgctgtc cgtgcaggcg gtcgtgttgt ggctgtcggt ctaccgccgg agtctatgag
     3361 cctggatatc ccacgtcttg tgctggatgg tattgaagtg gtcggttcgc tggtcggcac
     3421 gcgccaggat ctaaccgaag ccttccagtt tgccgccgaa ggtaaagtgg tgccgaaagt
     3481 cgccctgcgt ccgttagcgg acatcaacac catctttacc gagatggaag aaggcaaaat
     3541 ccgtggccgt atggtgattg atttccgccg ctaagaggcc tttgctgcga ctgccagtcc
     3601 tggtgtcgca gcatcgcaca ctctccaaca tgaaatggct aaatggatta ttgttgcgta
     3661 agaaggaaca ataatgatag tgttcccagt gaaggaacac tactgagagt aaagacacta
     3721 atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact ggacgaactt
     3781 aatgtcagcc tgcgcgagtt tgccagagca atggaaattg ctccctcaac ggcaagccga
     3841 ttgctgacag gaaaagcagc tttgacccca gaaatggcaa ttaaactttc cgtggtgatc
     3901 ggcagttcgc cgcaaatgtg gctgaatctg caaaacgcct ggagcctggc agaggctgaa
     3961 aaaacggtgg atgtgtcgcg actacgccgt ctggttacgc aataaaaaaa tggcacctta
     4021 taaagcgcca tttgaaactg atggcaaacg caacattgcc tgatgcgcta cgcttatcag
     4081 gcctacacgt tcactgcaat ctattgtatt tgaagggttt tcatgccgga taaggcattc
     4141 acgacgcatc cgggatgaac aaagcccacg ttgtctacta tatgaaaatg gcgcctttta
     4201 cagcgccatt ttcagcttaa agttcaaaag agattatatc ccttcttcac tctctttttt
     4261 cgcttctgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg cccagcgacg
     4321 gctcaccttc ccttcgatca tccctttaat cgatcctttc acccaaaatg ccatatacat
     4381 atggatcagg atggcgtgga tcaggatgat accggcagcc gcgtggatca gcaggctgta
     4441 gcgaacaacc tgcatcggga agtactgcgc aaagtacgga cgccagataa tcaccccggt
     4501 caccagcagc acgaaaatca tgctcatgat cgaccagaac atcatctttt gcccggcgtt
     4561 gtacttacca acatccgcca ctttatgctc attgcctttc aatacttcga caatgttcaa
     4621 cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca caaacataaa
     4681 catcagcgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc ccatctgcgg
     4741 cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa tcccggacag
     4801 cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa taaatttggt
     4861 gcgcacaatc attttcgact tactcatgat gatcctcctc gtcatcgtcc acttccttat
     4921 tcgggccaat accgatgtag tggaaaatca accctgcaaa agtggcaata aagccagccg
     4981 ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtgtcgatc ttcggatctt
     5041 tcggcagacc gtgatacagc tccggctgat cggcgtgatg cagcacgtac ataacgtgcg
     5101 taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc gctttcagtt
     5161 tcgccacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag tggatagccc
     5221 cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga tcgacgcaga
     5281 gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta aacggacacc
     5341 cggcaatgca gtaaccacag ccgatgcagt tttccgactg gaaatcgaca atcccgttag
     5401 cgtactgaat gattgcacca gcagacgggc acgccttcag gcagcctgga tcttcacagt
     5461 gcatacagcc gtctttacgg atcagccact ccagcttgcc gttctgttcg gtttcgctaa
     5521 agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa accccgacgc
     5581 agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga caggctttac
     5641 agccgataca ggtggaaacg tcgataagtt ttgcgacttc tgctttgtaa tcacgcacct
     5701 gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc gtttccatag
     5761 ccatctgttc gcccccttac gtcttctcga tgttgactaa gaacgcttta tattccggcg
     5821 tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa cctttacgcg
     5881 cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc tgaccattta
     5941 cattcagcgg tttcagacga cgcgttacca cagccaccgc gcggataaag ccacgcttgc
     6001 ttgagacagt gacacgatcg ccattattaa tgcctttcgc cgccgccagc gtttcgctga
     6061 tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg gtccaggtgt
     6121 ggaagtgctc ggtcagacga taggtcgtac ccacatacgg gaactgctct tttttaccca
     6181 tccgcagtgc gtcttgttca tacagacgaa caaccgggtt agacaccacg ttcggatgca
     6241 gcgggttagt gcccagcggc gtttcaatcg gctcgtagtg ttccgggaac ggaccttccg
     6301 ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata aacggcccgg
     6361 ttggcgtacc cggtgcggca ttgccgaagt caggaatatc gttacccgtc cacttgctgc
     6421 cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata tccgccgaag
     6481 cacggttgta cagcacgcga cggttgagcg gccacgccca ggcccatccc agcgtattcc
     6541 ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc tctgtccagc
     6601 taccggtgta gatccagcaa gaagatgcag ttgtaccgtc atcacgcaga tgcgcaaagc
     6661 tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcatcatag agatcttcca
     6721 gcgcatagcc gttgttctct ttagctactt cgtcagattg cggttcgtgc ggctgcttgt
     6781 agttccagct catcttcatc agcggttcta cgcctttacc accttcggcc tggtacagct
     6841 cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgcgct tcgcccggcg
     6901 catcctgacc tttccagtgc cactgcagcc agcgaccgga gttagcaata gaaccatctt
     6961 cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagac gccggatcga
     7021 catcgttcga ctcgccgtgg ttctgccaga aggttgaggt ttcagtcacc agcggatcga
     7081 taaccaccat gtacttcagt ttgctcaggc agctcaccac tttgtttttg tccgggaagg
     7141 acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttgccttca tccatcatgt
     7201 tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtca tagccccagt
     7261 tgttctcttt ctgcgcggca tcgccataga aagatttcat caggctaacg aagaacttcg
     7321 gatagttgct ccagtagttc acctgaccag ccagcgtcgc tttcggcgtg ttcgcttcca
     7381 gatacgactg caaatcaacc tgtttttctg acggcagcgt cagataacct ggcaggctgg
     7441 tagagagcag gcctaagtca gtcagcccct gaatgttgga gtgaccacgc aatgcgttca
     7501 cgccgccacc ggccataccc atgttaccga gtagcaactg gatcatcgcc atagtacgga
     7561 tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag gtggttgtgc
     7621 gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg gcttttggcg
     7681 taccgcagat gttttcaacg acgtccggcg tgtagcggga aacgtgctct ttcagcaggt
     7741 tccacacaca gcgcggatga gtcagtgttt catcgcgttt tgcatagccg ttttcatcga
     7801 actgatagtt ccaggacgac ttatcgtact ggcgtttttc agcgtcgtag ccgctgaaca
     7861 gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg taatgcttaa
     7921 cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca gacaggaacg
     7981 taatgtccgt accggaacga ataggtgcgt aaatatccgc cacagaagcg gtacgcgtaa
     8041 aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc atcgcccagc
     8101 ggaaaccgac gggatgcgct tcagcagcgt taccgcccat caccatcacg acgttagcgt
     8161 ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg
     8221 ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc ccgagggagc
     8281 gggcaaattt ctgggtcagc atcccggttt cgttgctggc accggaggca cacagcatac
     8341 cggtagaaag ccaacggttt accgttacac cctgctcgtt cttttcaata aagttagcgt
     8401 cacggtcagc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag ctaatgcgct
     8461 gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt tcactgttga
     8521 cgtaatccag caaaccggcc cctttcgggc acagcgcacc acggcttacc ggatgatccg
     8581 ggtcaccttc aatgtgataa atcgcttctc tggcgttttt tgcgccatca cccaggctat
     8641 acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc tctttagcgc
     8701 gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag cccaatgccg
     8761 ctactgttgt tccagccata ccgcccgcgc agattttaaa aaattgtctg cgactgacgt
     8821 ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca gtaaaattct
     8881 gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat ccgcctgcgc
     8941 aaattgacct acctcaatag cggtagaaaa acgcaccact gcctgacagg ccagttaaaa
     9001 aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag cgagcatgac
     9061 acgacaaaaa gcaacgctca taggactaat agcgatcgtc ctgtggagca cgatggtagg
     9121 attgattcgc ggtgtcagcg aggggctagg cccggtcggc ggcgcagctg ctatctattc
     9181 attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc aaatcccgaa
     9241 aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc tggcgctttc
     9301 cttagggtat gcggcgaccc gtcatcaggc gattgaagtg ggtatggtga actatctatg
     9361 gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca actggttgat
     9421 tgtaccagga ttattattag ccctcgtcgg cgtctgctgg gtgttaggcg gtgacaatgg
     9481 gttacattat gatgaaatca tcaataatat caccaccagc ccattgagtt atttcctggc
     9541 gttcattggt gcgtttatct gggcagccta ctgcacagta acgaataaat acgcacgcgg
     9601 atttaatgga attaccgttt ttgtcctgct aacgggagca agtctgtggg tttactattt
     9661 tctcacgcca caaccagaaa tggtatttag cacgccagtc atgattaaac tcatctctgc
     9721 ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatactgc atggcaacgt
     9781 caccataatg gcggtaggtt cgtattttac gcccgtactt tcctcagcgc tagcagccgt
     9841 gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctgatgg tctgcggcgg
     9901 ttccctgctc tgctggctgg cgacacgtcg tggctaaaat aattgccggg tctac
//
LOCUS       DAPGRN010000147         9948 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPGRN010000147 DAPGRN010000000
VERSION     DAPGRN010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.149"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="RHW33_004111"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="RHW33_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HDV1647563.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(415..627)
                     /gene="cspA"
                     /locus_tag="RHW33_004112"
     CDS             complement(415..627)
                     /gene="cspA"
                     /locus_tag="RHW33_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV1647564.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(908..1198)
                     /locus_tag="RHW33_004113"
     CDS             complement(908..1198)
                     /locus_tag="RHW33_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV1647565.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1632..2342
                     /locus_tag="RHW33_004114"
     CDS             1632..2342
                     /locus_tag="RHW33_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV1647566.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2392..3366)
                     /gene="ghrB"
                     /locus_tag="RHW33_004115"
     CDS             complement(2392..3366)
                     /gene="ghrB"
                     /locus_tag="RHW33_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV1647567.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVDQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3470..4129)
                     /locus_tag="RHW33_004116"
     CDS             complement(3470..4129)
                     /locus_tag="RHW33_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV1647568.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="RHW33_004117"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="RHW33_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV1647569.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVCDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMGWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTILMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALEKPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLEMFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="RHW33_004118"
     CDS             complement(6584..7024)
                     /locus_tag="RHW33_004118"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV1647570.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRECIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="RHW33_004119"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="RHW33_004119"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV1647571.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRKNYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7742..8440
                     /locus_tag="RHW33_004120"
     CDS             7742..8440
                     /locus_tag="RHW33_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1647572.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVVVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9871
                     /locus_tag="RHW33_004121"
     CDS             8669..9871
                     /locus_tag="RHW33_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647573.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTIASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLIISLALSTTIRQPQQ
                     KVLREAHI"
BASE COUNT         2356 a         2409 c         2575 g         2608 t
ORIGIN      
        1 agtgaggccc ctctatgcat agacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattgctttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 ggagttatga gctggcggca tttttagcct gcaatttgaa agagtaagag tcttcggcgg
      241 gaagttattc ccgcctttct tacggcgttg cgcattctca ttgcacccaa atttattttt
      301 cacaaaaata atactagatt ttattacgcg atcgattatt tatttcctga aaaacaaata
      361 aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca gagattacag
      421 gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga aggacacttt
      481 ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt gtacgaacac
      541 gtctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt
      601 tacgatacca gtcattttac cggacatagt gtattacctt taataattaa gtgtgccttt
      661 cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac tacgaggggt
      721 atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt ctgtacgtca
      781 aaccgttgat gtgcattaag ccacgcattg gcgggtgatg caacaattat ttttcatatt
      841 tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca ataaagtgga
      901 taaaagtcta ttccatcaac tgcttactta atgccgggtt ggcttgaatc aaacgcatca
      961 attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt accatggcga
     1021 ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa tcctgtccct tttcgtaact
     1081 gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc gtttcatctt
     1141 taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta tattccattg
     1201 aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt aagaatagtc
     1261 gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat tcgattcagc
     1321 cagtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat atatctctta
     1381 ccactcctgg tttgtaaaga tttcgaaaat ctaaccttct gcgaaggcgt aaatctctgg
     1441 ctacaaaaaa ggcaacggca atatcatttt ttaacaatga aacaacaaat taaccttgtt
     1501 tcattttctc catgatttta ccaggaagat tacgtagaaa gggtatcctg caagcctgtc
     1561 ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa
     1621 ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt
     1681 tattaatggt agttagcctg agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt
     1741 ttatcgactt cctgcagaat acagtgatgc gtagcggtga acgtctacca accctgactg
     1801 ccgatcagaa aaaacagttt ggtccttttg tctctgatta cgcgattctg tatggttatt
     1861 ctcagcaggt gaatcaggcg atggattccg gtctgcgccc ggttgtagac agcgttaacg
     1921 caattcgcgt accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa atgaatggtt
     1981 cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat gctgcgcact
     2041 ctgcgctaaa acagagtgat gacctgaaac cggtctttga tcaggcattc accaaagtgg
     2101 taacgacgcc agctgatgca ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac
     2161 aactggtgat ggttggagac tacattgccc aacagggtac tcaggtgagc tttgtagcaa
     2221 atggcatcca gttcccgact tcacagcagg caagtgaata taacaaactg attgcgccat
     2281 taccggcaca gcatcaggcg tttaatcagg cctggactac agcagttact gcaacccaat
     2341 aaagagtaaa agcccgagcg attatcgggc tttactccac gcagtcgcgg cttagtccgc
     2401 gacgtgcgga ttcacacagt tcttctcaac ctttccttgt aacgcatcaa tcaaattatc
     2461 cacggcacag gcggccatgc cataacgcgt ctcatgggtg gcagatccaa tatgcggtac
     2521 tgcgacgacg ttggccattg agagcaacgg cgaatctacg gaaagtggct cttgttcgaa
     2581 gacatccagc ccggcggcgt gaatttcgcc tttctgcaat gcagcaatca gtgcattttc
     2641 atcaaccacc gggccacgtc cggcattaat gaatatggcg gaggatttca ttttggcgaa
     2701 ttgttctgcg ccaaatagat gatacgtctc atcagttaac ggcaggatca ggcaaacgaa
     2761 atctgactct tgtaacagtg tatccaaatc gcagtagcgg gcgttgaagc gttcttctgc
     2821 ttctttatgg tggcggcgcg cgttatagag gatgggcatg ttgaagccaa agtgcgcacg
     2881 ttgtgccagc gccatgccga tccgtcccat cccgacaatg cccagtgttt tatggtgaac
     2941 gtcagtgccg taccagtccg ggcctatgct cgcggtccat tcgcctgctt ttacccgttc
     3001 tgctacctcc acaacccgac gagcggtaga caacaccagc gccatcagcg tatcggcgac
     3061 ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg taagcgcatc
     3121 gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg gcacgcagtt tcggcatttt
     3181 ttccagcaat gcggcatcta cattctcgtt tgaacccagt aaaccttcag cttcggcaaa
     3241 aattgctgca ttttgatcga cggtttgtgg gctgaggttt gccacctggt gaacggtgaa
     3301 atgctcttgc aggcgttgca gtaaatcatc aggtaaggct ttgtagagga taacggacgg
     3361 cttcatgctt ctctccattc actgataagg cgatcacctt agcctgaaat atgatgaagg
     3421 aaaactgaag aatgccaggt gatttctgca tcacctggca tgaaagggat tacagcgggc
     3481 ttaaggtaat ttctacacgg cggttttgcg ccttaccttc tgcggtgctg ttgctggcga
     3541 ttgggttagc cgggccaagg ccctgagtac ggatgcggct ggcgtccacg ccctgggtga
     3601 tcaacgcgct ggcaacggaa tccgcacgct gttgggagag acgcatgttc agatcgtgac
     3661 cacccgtgct gtcggtataa ccaatcacgt taaccgccgt tttcggatac tctttcagca
     3721 ccattgccac gccggtcagg gtgttagcgc ccgcaggttt cagggtcgcg ctgctgctgt
     3781 cgaaggtcac attgttcggc atattgagga taatgttatc cccgctgcgg gttacactaa
     3841 caccagtgcc gcgcattttg tcacgcagct tcgcttcctg cacatccatg taataaccca
     3901 cgccgccgcc cagagctgcg cctgctgctg cgccaatcag cgcgccttta ccgcgatctt
     3961 tcttcgaaga agagagcgca ccaatacccg cgcccacgag agagcccaga cctgcgccga
     4021 tagcagattt acctgcttcg cgttcgccgg tgtaagggtt agttgtgcag ccagataccg
     4081 ccagagcgcc actcactacg gcggcaataa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg ccacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatat
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccattggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg tttgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg cgcggcacag
     4741 gcgatcatgc cgtatgtcgt gggtggtagt gaagtttatc aacagcagac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ctgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttctgc actgcgtgac
     4921 agcgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg ggtgggtggc accgcacatg ggcactgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat actgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt agataaaatc
     5461 cctgtggccc gcattgttga agcactggaa aaacctggtg gcgcatatca acacaacggt
     5521 atggaccgac attttccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaaccg cctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tccgaatgtt tctggacggc ggcggcgaaa cacgcggata tcgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttaagtgaac gctgggagaa gggcggctat gcgcggttta cggaaggaaa aagtgagctg
     5881 caatggctgg aaatgtttta taacgttgcc cgacagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aatcagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgcaggc gcatccatta
     6061 aaaaccgcca gcggtaagat tgaaatcttc tcgcagcgta ttgccgatta cgcttatccg
     6121 gattgcccgg ggcatccaat gtggctggag ccggacgaat ggcagggtaa tgccgaaccg
     6181 gaacagttac aggtactttc tgctcatccg gcgcatcgtc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtgaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat acaagatggc gatactgttc ggctgtggaa ctcacgcggg
     6361 caaattcttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 gcgctggcat ggctagaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcctgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatggcgc tgttgcacat actgcatcag cgccttacca ataccgcgcc
     6781 tgacggcctt cggtgcgaca aacatcgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttactgtct tcttcccaga cccagttttg tgcgttggca agataggcat
     6901 cccgcaccag cggaatgcac tcgcgccagt aattcgcttt tataaaggga tggccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttccctaa
     7021 tcatggttta tttcccggat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt accgacaaac ttaaaaccac gttttttcag
     7141 tgcctttgat agtgcgtcgg aggcgggcgt agatgtgggg atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcggcaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttcccgcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgcggc
     7381 aaccttcacc ggatcgaact gatgaaagca ggcgcgatag tttttgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttccaggcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggtcctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gtgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccgggcat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaacc cctgtggttg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaactggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtacctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgccgcgct atctcaggca gaaaatacca ctaacaatag
     8401 cgattatctg tatacgatgg gggttagtgc cagattttaa cgtaacaatc acaattgaaa
     8461 ccattgaata acaatagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgtcatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccag gcataaagtg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gttgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacctggag
     8761 cctgtttaac ggcgcgcttt ccgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat atcgtcttct gttgcgggca aattgcagga
     8881 acgttttggt gttaaacgcg tcaccatcgc ttccggcatt ttgctgggat tagggttctt
     8941 cctgacagcg cattctgaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaaat ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctggaaac ggtcggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 tgcagccaat gcagtcactg ttatttccat cgccaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaatcg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attgaatgca gtgacgttct ttgcagcgat
     9601 tgcctgtgtg gcatttaact ttggcggcac tattaccgtc tttccgtcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tgtggttcga ttatcgcctc actgtttggc ggcttctatg tgacgttcta
     9781 cgtcatcttt gctctgctga ttatttccct ggcactttcc accaccattc gccagccgca
     9841 gcagaaagtg ttgcgtgagg cgcatattta atgagatggc gttggttatt aacgaagggg
     9901 cgagagagcc cctttttttc gtttaaaata ataaggagat tacaaaac
//
LOCUS       DAPGRN010000148         9931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPGRN010000148 DAPGRN010000000
VERSION     DAPGRN010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.150"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..192)
                     /locus_tag="RHW33_004122"
     CDS             complement(<1..192)
                     /locus_tag="RHW33_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647574.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIY"
     gene            270..1364
                     /gene="ddlA"
                     /locus_tag="RHW33_004123"
     CDS             270..1364
                     /gene="ddlA"
                     /locus_tag="RHW33_004123"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV1647575.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1388..1600)
                     /locus_tag="RHW33_004124"
     CDS             complement(1388..1600)
                     /locus_tag="RHW33_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV1647576.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1860..2168
                     /locus_tag="RHW33_004125"
     CDS             1860..2168
                     /locus_tag="RHW33_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV1647577.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="RHW33_004126"
     CDS             complement(2227..3321)
                     /gene="yaiW"
                     /locus_tag="RHW33_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV1647578.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="RHW33_004127"
     CDS             complement(3334..4554)
                     /gene="sbmA"
                     /locus_tag="RHW33_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV1647579.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4906..6063
                     /gene="ampH"
                     /locus_tag="RHW33_004128"
     CDS             4906..6063
                     /gene="ampH"
                     /locus_tag="RHW33_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV1647580.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6064..6687)
                     /gene="iprA"
                     /locus_tag="RHW33_004129"
     CDS             complement(6064..6687)
                     /gene="iprA"
                     /locus_tag="RHW33_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV1647581.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6775..9753)
                     /locus_tag="RHW33_004130"
     CDS             complement(6775..9753)
                     /locus_tag="RHW33_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1647582.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGAISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMGESSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANST
                     IDDDYEGLYFTDSYLDGDVTKYTNETFRTPAGEGEEYAGLFANGGVGLGLAVNLDVES
                     NINISNNSRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFYG
                     NSAEPSDYSGNGGANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFYP
                     QGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSKWIGSATTTTNVDVESTASTDWY
                     DVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNNG
                     SQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNGY
                     YTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIHS
                     SDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVV
                     AATGNYKVRIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT         2623 a         2446 c         2315 g         2547 t
ORIGIN      
        1 ataaataaaa gttatgaaat atattctctt cgttaactta taattaagag aaaaaaactc
       61 accgcatggc catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt
      121 gcgcacatct ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga
      181 agattgcaac aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca
      241 ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg
      301 gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca
      361 ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg
      421 tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc
      481 cttcggcgac cagcctcgcc caggtgccag gtaaacatga gcatcagctt atcgacgcgc
      541 aaaacggtca gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg
      601 gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg
      661 atgttctggc ttcagcagcc tgtatggata aagatgtaac caaacgtctg ctacgcgatg
      721 ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt
      781 ttgccgaagt ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct
      841 cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg
      901 cgttcgagtt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat
      961 gcgcagttct gggcaacgac aatccgcaag ccagcacctg tggcgaaatc gtactcacca
     1021 gcgatttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc
     1081 cggcagccat tgcgccagaa atcaacgata agatccgggc gattgccgtt caggcttatc
     1141 agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag
     1201 tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc
     1261 tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc
     1321 tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat
     1381 aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca
     1441 gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt
     1501 caaatcccag taaccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat
     1561 agtagtgcca gtcacggcgg atttttacag gcagattcat ggctgctccg ttaacgaaaa
     1621 acaacatcct tgcatattct gcaacaaccg tctgtggtgt agacaacatt ttcgcaattg
     1681 ttaataaatt taacttaaag ttgctatttt gtgactcaca gcgaagattc acgcttcggg
     1741 aatattccca acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga
     1801 cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta
     1861 tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa
     1921 cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat
     1981 tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa
     2041 caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt
     2101 ttttagtagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc
     2161 gccagtaaga tttacgttgc cagatacggc ctaaaacgcc ttatctggca tgacattcct
     2221 gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc
     2281 cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg
     2341 ggggagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt
     2401 ctcctcaaaa gagaagctgt cacctttctc tagctgacga cgaatttcgc tgtcgctcat
     2461 ccccagtttt gcagccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc
     2521 gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgaccgcgtt
     2581 ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa aacggtatat
     2641 cggtgcgcta taactggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc
     2701 gcggcgacta aagacttcct gacggactgt accgtccatt ttccacggat accctttggt
     2761 atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag
     2821 cgaaccaaac agcgtctgcc ccattggcac catgctaatc agatcgtcaa aaatcgcgct
     2881 caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga
     2941 tttgattttt agcgcggtat gaacaagaaa aggaggaatg tgcatccgtt cagcacgacg
     3001 gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt
     3061 tgactcctgc tgcgccaccg ccagcaccga gcagacgttt tccagcgtcg gtgccagccc
     3121 ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc
     3181 aggcatcttc tgccgcacga cgctcgccac atctatcgct ttttcgccct ttttcaacgg
     3241 ttgcggagcc tgactactgc accccgctaa aaccagaacc gccaacaatg acagcgaaga
     3301 tagaggattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg
     3361 aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa
     3421 ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa
     3481 aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa
     3541 cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa
     3601 atacatatag tgaaaataga ggcggaaata gtttttccgt acggcgctaa acagctcgcg
     3661 taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta
     3721 ggcagcctct actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc
     3781 cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat
     3841 gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa
     3901 ggcgatcaac gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc
     3961 aaaacgcatg gtgtcttcct gcacacgctg tgcggcgcct tcgatatgac gcagttgttg
     4021 ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac
     4081 aaagaagtta ttcagcacac tgatcactac cgcgatcagc gcaatcccca gaaagacgcc
     4141 cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat
     4201 cagatcatag aacggtgcat accaggcgtt gacggcaacc ccgacttcca ccaaaaacca
     4261 ggtgacgaag atgatcagtg cagtaccgag tattgaccag tattgccaac ggtgcgggct
     4321 gtagataaac cagaacaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat
     4381 caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt
     4441 gatacgcgcc acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca
     4501 aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcgc
     4561 tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa
     4621 gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct
     4681 gaccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta
     4741 acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc
     4801 cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag
     4861 tataaatacg ctcagttacc ttcattcatt ttatggacac caccgttgaa acgtagtctg
     4921 cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc
     4981 actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc
     5041 agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgcagt
     5101 tatggcgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt
     5161 gcttccctca ccaagctaat gaccagtgaa atgctggtga aattgctcga ccaggggacc
     5221 gtgaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccaacctac
     5281 aatgggacgc ccattacgct ggtcaatctg gcaacccata ccagcgccct gccccgagaa
     5341 cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg
     5401 aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac
     5461 cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag
     5521 ttgtttgaag agcagattac ccgtccattg gggatgaaag acaccaccta caccccatcg
     5581 ccagaccagt gccgccgttt aatggttgca gagcgcggtg ccagtccatg caataacacg
     5641 ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg
     5701 cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg
     5761 ttgatttatc agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg
     5821 gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc ggggattatt
     5881 cagaagacag gtggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat
     5941 attggcgcgt ttgtggtggt cactcgttcg ccattgacgc gctttaaaaa tatgagcgat
     6001 ggcatcaatg acctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc
     6061 tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat
     6121 atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt
     6181 acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat
     6241 cattgataat aatgttgcgc ggatttgttc gtaggaatta tgcccaatga gctgcacgtc
     6301 gcgtaattcc agaattctat tttgccaggc taaccagtaa aaagcgtctc gccagagttg
     6361 attttgttca ataagcgtaa tggtttgttt ggctggtaga tgatatcccg tacaatttcc
     6421 ttctgatatt aatttatatg gtatatcgtt tttcattaaa ccatcagcca gccccataat
     6481 ataaggagcc tgggtaatac cgataagtac gttttcttct ctacgcagag agataacgcc
     6541 ctctaaaata acgaaagtat cttcgttatt gacatcactg gaaaatataa cttgcttttc
     6601 attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc
     6661 ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc
     6721 cttgacgggc attatttaga ttaaattacc agtatttctt cggagtgaag aatattacca
     6781 agtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta
     6841 gttagcatcg gtataggcgc tgaagttctt agtgaagctg aactgagtac ccagaccaac
     6901 acgtaccgca gacccttcag taccgttatc gatggaatca ccgttcacat cgttatcgtt
     6961 gttagagtcg tcgtagacgt aagccagttt gaagtacgga gtcagagcct ggtcttcgct
     7021 gtaagtgaag gtataacctg catctacacc cagttcataa cgcatgctgt cgtaagactg
     7081 accgtcaact ttcatgtcgt tgctcagctg gtagtcatca ccagactgga acagaccaga
     7141 aatgctgccg taaggagtca cgtaaccagc atcacccagt ttgaagtcgt aaccggcttt
     7201 caaaccgaag ccccaagcgt cggagttggt gctaccgtca acgtaagtac cgttgctcat
     7261 ggttgcagac aggtcgttgt tgaagtgaga gtagctcaag ctaccatcaa caaagacgtt
     7321 gttcgcaaag tgagcagaag agtagatgta ggcagtctgg ctgtcttgat ccacctgacc
     7381 agaacggtca ttcatgtcac ctttagcgaa gcctgcagcc gcaccgacga tccacttagc
     7441 gttgttaccg tcaattttgg tatcaacacc gaccatgatg ccgttaacat cctgatcata
     7501 gttgatggtg ccgttgtcgc cgttgaagtt accaccgaag tagcttaccc atgcgccgcc
     7561 gttatcagcc aggccatgac gagagttggt caaacgagta ccaacggtgt cttgttccag
     7621 gttccagata ttggtgttcg cagacgggat gctcagcgcc atgttagcgt agtcggtcag
     7681 ctccatctgt tgcagaacaa cggtgttacc gcgctgttca gcctgatagg tgtatgcacc
     7741 caggtcagct ttgttagcag cagagaaggt cgcgttggtg ttgacgtcgt ttacgtagat
     7801 aatttcttta tctttgtaat cagcgatagc gccagcgcct gttgcgttgt cgatacgaac
     7861 tttgtagtta ccggttgcag caacaacgtt gtcaacagag ctgttatcca gttcgtcgcc
     7921 gttgttcatg tcaccgttac cgttgatagt cagatgacca tcagagttca tagcaacaat
     7981 accgtagccg tagttagact tggaagtatc ccaggtacgg tcgttcacca aatcagcgtt
     8041 cagaacataa tcactgctgt ggatatcgaa tacaccagac actacgttgc cgttatggtc
     8101 agcgatgttg ccgctggtca gttccaaagt atcagtctgg aacggattat catcgaactg
     8161 gtctacatta atatccagta aaccaccgtt agtcacagtt attgtatttg ctaacagcga
     8221 cggagtgtaa taaccattat agatacttga attgtcatca ctagacagcg ctacagtact
     8281 gtagctatca actgtcagat gatcagttcc aacataacct tcatccccaa tgttcaaaga
     8341 agaaccattg gtcagagcga tgctatcaga aatcaagcta gattcagaca cattaacctg
     8401 agagccatta ttcacagcta aagtatcaat gtttgaagcg ttaacggtat tccattcaga
     8461 accattattc agagtgacgt taaacacacc gctctggaat acttgatcat caatcgtacg
     8521 accccaagca tttgcttctg ttacaacgcc tggatacagg ctattacctg taacatcata
     8581 ccagtcagtc gaagcagtag attcgacatc aacattggta gttgtagtcg cagaaccaat
     8641 ccacttagaa ccattatcca gagtgatatt cagttcatca acattaagtg agtcgtcagc
     8701 ccagccacca ttggtgtcca gtacgccatc agcattactg tcatgacctt gcgggtagaa
     8761 gtttgcgttg aaagtactcg cgaaaacaac atcacccagc agagtagaat tgctgaaata
     8821 aacattgttt ttcatggagt aatttgaagc cgcactatca ctgaaataca gagctacatc
     8881 attagctccg ccgttaccag agtaatcact aggttccgca gagttaccat agaaaccact
     8941 gtcttccaga gtagttacag aacctgaagt cactgtggaa tcggtaactg agatattgtt
     9001 gtcccaagta tgagattcag tagtgtaagt attgtttaca gtattaccct gagtcaggct
     9061 gataccagca acacgggagt tgttagaaat attgatgtta ctttcgacat caagattcac
     9121 tgccagccca agaccgacac caccgtttgc aaacaaaccg gcgtattctt caccttcacc
     9181 agctggggta cggaaggttt cgttagtata tttggtaacg tcaccatcca gatatgaatc
     9241 agtgaagtat aaaccttcgt aatcgtcatc aattgtagaa ttagcaatat ttaaagtgaa
     9301 tacatcacca tcatgccaat tatcatcaat tacgcgatca gtaccgtatt cagagaactg
     9361 atatacataa tgaccatcac tgctttcacc cataggcagc attgaagtga ttgaaccatg
     9421 aatcagggag ttgctgatgt tcaactggtt tgttgttcca tcagtaaagt catgattcag
     9481 gtaatatgta gagatagccc cgttaacagt gtcaccatta aaggtggtat cgaaatcacc
     9541 gtttgtgttg ttatagtaga caaagccact ataattcaca tagttaccat ctacagtacc
     9601 cggtgcatcc atattgctcc agtcaagagc agtagttgga ttgtcgtaat aagtccaggt
     9661 gtcataagta gttgcattag cctgagaggt gatagccaga gtgcaagcca atgctaattg
     9721 tgatactaca agtttctttt tccaggagtg catttgtcat ccctcctcag ggacgtaata
     9781 agttgatcca tcaattatca atgcatagaa aacatcttat gtttcgcgtt tacgattatg
     9841 cggcgttata gaggaatggt tcaattattc tttcccctaa gtccgattgc ggacatatat
     9901 aaaaatatct atacgcacaa agaataagtt g
//
LOCUS       DAPGRN010000149         9886 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPGRN010000149 DAPGRN010000000
VERSION     DAPGRN010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.151"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..260
                     /locus_tag="RHW33_004131"
     CDS             <1..260
                     /locus_tag="RHW33_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1647583.1"
                     /translation="RGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQACGYTAN
                     ADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            304..411
                     /locus_tag="RHW33_004132"
                     /pseudo
     CDS             304..411
                     /locus_tag="RHW33_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(395..1033)
                     /gene="ytfB"
                     /locus_tag="RHW33_004133"
     CDS             complement(395..1033)
                     /gene="ytfB"
                     /locus_tag="RHW33_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV1647584.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1251..1871
                     /gene="fklB"
                     /locus_tag="RHW33_004134"
     CDS             1251..1871
                     /gene="fklB"
                     /locus_tag="RHW33_004134"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV1647585.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2180..3592
                     /gene="cycA"
                     /locus_tag="RHW33_004135"
                     /gene_synonym="dagA"
     CDS             2180..3592
                     /gene="cycA"
                     /locus_tag="RHW33_004135"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV1647586.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3637..4299)
                     /gene="ytfE"
                     /locus_tag="RHW33_004136"
     CDS             complement(3637..4299)
                     /gene="ytfE"
                     /locus_tag="RHW33_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV1647587.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4407..5372)
                     /locus_tag="RHW33_004137"
     CDS             complement(4407..5372)
                     /locus_tag="RHW33_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV1647588.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5480..6340)
                     /gene="qorB"
                     /locus_tag="RHW33_004138"
     CDS             complement(5480..6340)
                     /gene="qorB"
                     /locus_tag="RHW33_004138"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV1647589.1"
                     /translation="MIAITGATGQLGHYVIESLVKTVPASQIVAIARNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELVGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6429..6809
                     /locus_tag="RHW33_004139"
     CDS             6429..6809
                     /locus_tag="RHW33_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV1647590.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6927..8870)
                     /gene="cpdB"
                     /locus_tag="RHW33_004140"
     CDS             complement(6927..8870)
                     /gene="cpdB"
                     /locus_tag="RHW33_004140"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV1647591.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9060..9800
                     /gene="cysQ"
                     /locus_tag="RHW33_004141"
     CDS             9060..9800
                     /gene="cysQ"
                     /locus_tag="RHW33_004141"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV1647592.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2252 a         2530 c         2616 g         2488 t
ORIGIN      
        1 ggcgtggcgg tcaggtgctt gctgttccgc cagcgtacac aagccagcgt tgcgcgtgct
       61 gtggtcatac agcgaaagaa aatcgcctgt cacaaagtaa attcagatgc caggcatgtg
      121 gatatacagc gaacgctgat gtaaatggcg ctcgtaacat tttagcggcg gggcacgccg
      181 ttcttgcctg tggagagatg gtgcagtcag gccgcccgtt gaagcaggaa cccaccgaaa
      241 tgattcaggc gacagcctga acgtagcagg gatccacgtc cttcagggcg tggaggatgt
      301 caacacatca gtatggtttc accccaggca caggaagcca gacgtaaggt catcaaagag
      361 acaatcctta gctggctttt ggttgatcct tcatctaccg cgcacgaata aaactgccat
      421 ccggctggcg ggtaaacagg acctgttgat tattccccgt atcaatggtt aagcccgtca
      481 ccacgccgct ggcgttctgg cggattttca ccatctgccc attttgcaaa ttactcagcg
      541 gtttacccgc gccttctacc tgcgccattg catagacatc ggtagcgggc agtccgtggt
      601 cgcggaacag ctgcgccatc gttttaccgg gttcgacacg gtaagagcgc cattgattat
      661 caattccgct atctggctgg aatggttgtg tttgggtagt ttgcggctgc tcttctggct
      721 ggccttcctg tataggttct ggagcgaccg gagccacctg atcaggatca ttttgtggtg
      781 tgactaattg cgcccgcagc tgttcttctg tcggcggttg tgattgtgac tgaatatcta
      841 actgcgcttc acgcgtcacc accggcgcgt tgggcgtatc atcagatgga agcagaaatc
      901 cgaccaccaa tacaatggcg gcaataatga tcccccgacg atgcatcggc ggcagcgggt
      961 ccatgaagcg aaaattgtcc ggcgcgtgcc agactttctc cagggttggt tttagttcaa
     1021 agcgcccggg catggtttcc tcctgctccg cgtcttgttc ctcaatcata gcctatgaat
     1081 aagctaacgc tatgatgtcc gtggtaaacc cgcctttatt atattcatac gggatattgc
     1141 tattgtttct ttttccctgg gatttgtcat cattcccgcg acaaagtttt acccaaagaa
     1201 gtgtggctga tatgctgccc gctactttat accctaagaa aggaaatacg atgaccaccc
     1261 caacttttga caccatcgaa gcgcaagcaa gctacggcat tggtttgcag gtagggcaac
     1321 aactgagtga atctggcctg gaagggctgc tgccagaagc actggttgca ggtattgccg
     1381 atgcgctgga aggcaaacat ccggctgttc cggttgatgt ggtgcatcgc gcgctgcgtg
     1441 aaatccacga gcgcgccgat gccgttcgtc gtcagcgttt ccaggcgatg gctgctgaag
     1501 gtgtgaaata cctggaagaa aacgccaaaa aagaaggtgt gaatagcacc gaatctggcc
     1561 tgcaattccg cgtgatcaac cagggtgaag gcgcaattcc ggcacgtacc gaccgcgttc
     1621 gtgttcatta caccggtaaa ctgatcgacg gcaccgtgtt tgacagctct gttgctcgtg
     1681 gtgaacccgc tgaattcccg gttaatggcg tgatccctgg ctggattgaa gcactgactc
     1741 tgatgccggt aggttctaaa tgggaactga ctatcccgca ggaactggca tatggcgagc
     1801 gtggcgcagg cgcgtccatc cctccgttca gcaccctggt gtttgaagtc gaactgctgg
     1861 aaatcctcta agcagcgcat tctgttcccc tcgagccaga ggggagcagg cattcagcaa
     1921 taaacccttc agtttgccaa acggcgctat tttgtgttgc aaagaccgcg taagcgtgta
     1981 tttttgtgag ctgtttcgcg ttatcaccgt gatatgacac tcactttaaa cataaaatta
     2041 acattagatc taaatcttag tattcatccc gcgtattgtt acctaatatc gatgagtccc
     2101 gatacagatt cgtcgtatca tagactgact aaaggccgta gagcctgaac aacacagaca
     2161 ggtacaggaa gaaaaaaaca tggtagatca ggtaaaagtc gttgccgatg atcaggctcc
     2221 ggctgaacag tcgctacggc gcaatctcac aaaccgacat attcagctta ttgccattgg
     2281 cggtgccatt ggtacggggt tgtttatggg gtctggcaaa acgattagcc ttgccgggcc
     2341 gtcgatcatt ttcgtttata tgatcattgg ttttatgctc tttttcgtga tgcgggcaat
     2401 gggggaattg ctgctttcga atctggaata caaatctttt agtgacttcg cttccgattt
     2461 actcgggccg tgggcaggat atttcaccgg ctggacttac tggttctgct gggttgtaac
     2521 cggtatggca gacgtggtgg cgatcacggc ttatgctcag ttctggttcc ccgatctctc
     2581 cgactgggtc gcctcgctgg cggtgatagt gctgctgctg acgcttaacc tcgccaccgt
     2641 gaaaatgttc ggtgagatgg agttctggtt tgcgatgatc aaaatcgtcg ccattgtgtc
     2701 gctgattgtc gtcggcctgg tcatggtggc gatgcacttt cagtcaccga ctggtgtgga
     2761 agcgtcattc gcgcatttgt ggaacgatgg cggatggttc ccgaaaggtt taagtggctt
     2821 ctttgccgga ttccagatag cggttttcgc tttcgtgggg attgagctgg taggtacaac
     2881 agctgcggaa accaaagatc cagagaaatc actgccacgc gcgattaact ccattccgat
     2941 ccgtatcatt atgttctacg tcttcgcgct gattgtgatt atgtccgtga cgccgtggag
     3001 ttcggtagtc ccggagaaaa gcccgtttgt tgaactgttt gtgttggtag gtttgcctgc
     3061 ggctgccagc gtgatcaact ttgtggtgct gacctctgcg gcgtcttccg ctaacagcgg
     3121 cgtcttctct accagccgta tgctgtttgg tctggcgcag gaaggtgttg cgccgaaagc
     3181 gttcgctaaa ctttctaagc gcgcagtacc cgcgaaaggg ctgaccttct cctgcatctg
     3241 cctgctcggc ggcgtggtga tgctgtatgt gaatcccagc gtgattggcg cgttcacgat
     3301 gatcacgacc gtttccgcga ttctgtttat gttcgtctgg acgattatcc tttgctcgta
     3361 ccttgtgtac cgcaaacagc gtcctcatct gcatgagaag tcgatctaca agatgccgct
     3421 cggcaagctg atgtgctggg tatgtatggc gttctttgtg ttcgtggtcg tgttgctgac
     3481 actggaagat gacactcgcc aggcgctgct ggttaccccg ctgtggttta tcgcgctggg
     3541 gctgggctgg ttgtttattg gtaagaagcg ggctgctgaa ttgcggaaat aaccgcatta
     3601 tcatgctgga tggcgcaatg ccatccagct tttagatcac tcacccgcca gcgcgcgtgg
     3661 gaacagtaca ttgttttcca gactgatgtg atccatcagg tcatcaatca gttcattaat
     3721 tccgttatac atcgctttcc aggtggtgca ggcttctggc ggcggtgtga cgttattggt
     3781 ggtgtgttta atcacttcca gcagttcgcc cgcttcatcg tgctcgcttt ccattacgct
     3841 gattggcccc attgcctggc tgcccatgcc ttgtttgatc atcgggaaga ggatctgctc
     3901 ttctttcatc atgtggctgg aaagctcttc atgcagcata gtcaggtatt ttgtcagccc
     3961 ttttggcacg ctcggtttgt cggcgtgaac gcgctcgact ttagtcgctt gcaggatcag
     4021 ctccggcaac tgctcgcggt gacgatcgtg gtagcgcacg atgatatggt caatgatttc
     4081 tgccagcggg gcgctacgcc agtctttctc aatcggttgt tcagcaagct ttgccagctc
     4141 agcttcaatg acctcaacat ccagttcttt acgtgccgcc gcgcgcgcca gcgtctgctt
     4201 accgccacag cagtaatcca tatcatattt acgaaacaga gccgaagcgc gagggataga
     4261 gagcgccagt tcacctaaag gttggtcgcg ataagccata gctgatacct cattcttaat
     4321 aagataagat gtattttaaa tacatcttta aggcaaaagc tataaccctt acgtagcaga
     4381 ggcttattta actcactgcc agcgtgtcaa ctttctgaga cagattcagt taaaggtttt
     4441 tctggttttg ctctgaccgc tatgaccacg ccaatcacta acagcgcgat accgctcagc
     4501 gtcattagcg gcggcatttg ctggcggagt aaaaaggtgt acagcaaacc tgccagcgtt
     4561 tcgaaaacaa tcagcggccc gagaatcact gtcggtaatc gctggctggc gacgttccag
     4621 cagagtgcgc caacccatga gcaaagcacg gctatcgcaa ccatcagact aataaacacc
     4681 agcggacggg ggccaaaggg tagggagaag tccggcgttt gcgtattcag ccagtaacaa
     4741 gcgacgagat agccgataag tgaaaccggc agtgtgacca gcgcctgcgc tgttgcccac
     4801 atcatcggat gtttgtcggg attttcccgc agccagcggg cgttgcgcag ggcataccac
     4861 gcccagcaga ccacggaaac taacgccagc acgatgccag aggtataacg tgcccagtca
     4921 aaatcgggga gtccgtggtt taactcagca atattcacac acgccaggcc gatgccaata
     4981 caaatcagtg ccggggcgag ttttccccac gcgagtttgc cgtcgcgctg gctataaagc
     5041 agattggcga agacgggaat caccaccggc agggtgccga taatcatcgt ggaaacaggc
     5101 gcgccagtac gttgaatggc actggcaagg cagaagtaat aaatgaggtt gcccatcata
     5161 gtgagcatca aggcggtaag ccagtcgcga cgcgccaact gacgcagacg cacgcgtccc
     5221 agccaggcaa tgggcagcgc aattaaccct aacgccagat aacgccccat cgactgcagc
     5281 atcgccgggt attccggcac gatcaacggc ccgacaaaaa taagccccca catcaaccct
     5341 gctaacaggg cgtacagcac gccgctaatc attactggca tccattgatc tgtcagaaga
     5401 aagttcagga agcagacatg attgctcccg gcatcccgtc attatcagag aggatgcggg
     5461 aggatgccac tttaattaac tagttattaa cattaaaaag atggcttacg ctttcggcta
     5521 acgttgtcgt tgggcggcca atcaatttgc taagcgtttt gctgtcatca aatagaccgc
     5581 ctttcgatgc gccaacgtca gaatccgcca gcatatccgc cagtccgtcg ggcagtccga
     5641 cgcttttcag tgccgcggcg aaatcggctt cgctcagatt ttgataggta acctgtttgc
     5701 cgctctgttt ggtcagctct gccgctaact gtgtcaacgt ccaggcacta tcgcccacca
     5761 gttcgtaaac cttgccttcg tgaccggctt cgctaatcac gcgtgccgca gctgccgcat
     5821 aatctgcccg cgttgctgag gcaattttgc catcgcccgc ggcaccgata aatacgccgt
     5881 gctccagtgc tgccggtgcg ctggcgaggt agttttcgct gtaccagccg ttgcgcagca
     5941 gggtgtaaac gatgccagaa tcagccagca ttttctccgt ctcgatgtgc tcatcggcga
     6001 ggccgagcgg agatttatcc gcgtgaagca gactggtata agcgataaat ttcacgcccg
     6061 ccgccttcgc cgcgttaatc acattgcgat gctgcggggc acgctggccc acttcgctgg
     6121 aagagatcag cagtagtttt tccactccct gaagtgcaga tgtcagtgcg gcttcatcgc
     6181 cgtagtcagc ctgacgcacg gtaatgcctt gtgctgccag ggcctgggct tttgccgggt
     6241 tacgtgcgat agccactatt tggctggcag gaacggtttt caccagggac tcaataacat
     6301 agtgaccaag ttggccagtg gcaccagtaa tagcgatcat gggaagtctc catcgttttg
     6361 tcttgtgttt aaatacgcta acacctaaac ttacttttag taagtacgca caaaaaggta
     6421 agtatggaat gagtcaggtt agcctgtcgc aacaactgaa agagggtaat ctctttgcgg
     6481 aacagtgccc gtcgcgcgag gtgttgaaac acgtcaccag ccgttggggg gtgttgattc
     6541 tggtggcgct acgcgaaggt actcatcgct ttagcgacct gcggcgcaaa atcggcgggg
     6601 tgagcgaaaa gatgcttgcg cagtcgttac aggcgttaga acaggatggt tttcttaacc
     6661 gtatcgcgta tccggtggtg ccaccgcatg ttgaatacag cctcacgccg ctgggcgagc
     6721 aggtgagcga aaaggttgct gcactggcgg actggattga gttgaatttg cccgaggtgt
     6781 tggcggtgcg ggatgaacgt gcggcataac tgccggatgc gcggcctacg gtgtatgact
     6841 tgtagggcgg ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcgattgat
     6901 gatttatagg ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac
     6961 ccgatatcat cggtcgcgac tttattcatc ggatactgcc ctttctcttt aataaacgct
     7021 gcggctttat ctgacggaga ggtttcgaaa cggatatcca gtttcttatc gccagctatc
     7081 ggtgctaaac gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca
     7141 tcagcaatcc acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg
     7201 ctgtcgcccg taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg
     7261 aacatagcgt tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca
     7321 ttggcattaa tcatctggca ctcgccgtca taacgggcgg gctgggtaac atcaatctga
     7381 taattcacac catcaataac atcaaagtta taggtgcgga aaccgtccca gttgatgagt
     7441 gactgtggtt tcgtgctgtt gggatcaatc tggttaaact gtccggcgga gcactccagc
     7501 cactctttca cctctttacc gctggctttc accacaatca gcgtattggg atagagataa
     7561 agatcggcgg cattacggaa agtcagctga cctttttcca cctctacata gcttgccggg
     7621 tcatttttgc gaccaccaac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc
     7681 agatccggat cgccctgaat gtaatgttcg acatacgctt tttgcgcgtt gttcactact
     7741 tgcacggtcg gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta
     7801 ccgattggct tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc
     7861 agcttgctgt cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgtgct
     7921 tccgctttcg cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc
     7981 accccaagat gatcgcccca catgcccggc attaccgccg gaacaccatt cagcgtgcct
     8041 ttggtgatat cagccccttc gatatcagca aaatctttac ccgggaaaac ggcgtgagca
     8101 tggccaaaca taatggcgtt aacgcccgga atttcactga ggtaataaac tgagttttcc
     8161 gccattactt tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgacatcg
     8221 gcacctttct cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc
     8281 gtcacttttc cggataaatt agctttatcc cagcccatga tttgcggcgg cacgacgcca
     8341 atatagccaa tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta
     8401 attaaatacg gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca
     8461 taagggaatt tcgctcctgc cagcgcattt ttcaggtaat ccagaccgta gttaaactcg
     8521 tggttgccaa gcgttccgac ggtatagtcc agcgtattta atgccttata gaccgggtga
     8581 atatcacctg cttttaatcc tttcgccgac atgtaatcgg ccagcggact cccctgaatc
     8641 aaatcgccgt tatcgaccag tacgctgttt ttaacttcat tgcgggcatc gttaatcagg
     8701 cttgccgtac gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc
     8761 atcatgttgc tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc
     8821 acactggcgg caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc
     8881 cttttatcat cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg
     8941 aagaatgcca caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt
     9001 aacgataggg tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa
     9061 tgttagatca agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg
     9121 acgggacgaa accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg
     9181 atattgccgc acacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg
     9241 tcctttctga agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc
     9301 tggtagaccc gctggatggt actaaagagt ttattaaacg taatggcgaa ttcaccgtta
     9361 acattgcgct cattgaccat ggcaaaccga ttttaggcgt ggtgtatgcg ccggtaatga
     9421 acgtaatgta cagcgcggca gaaggcaaag cgtggaaaga agagtgcggt gtgcgcaagc
     9481 agattcaggt ccgcgatgcg cgcccgccgc tggtggttat cagccgttcc catgcagatg
     9541 cggagctgaa agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt
     9601 cgctgaaatt ctgcctggtg gcggaaggac aggcgcagct gtacccgcgc ttcggaccaa
     9661 cgaatatttg ggacaccgcc gctggacatg ctgtggctgc agctgccgga gcgcatgttc
     9721 acgactggca gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatccggggt
     9781 tcagagtgtc tatttactaa attcagatgg cagaaacaga gtatttcctg attctgccat
     9841 cctgatttct cccaacctaa aaagttataa ataaaaagag attgta
//
LOCUS       DAPGRN010000150         9657 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPGRN010000150 DAPGRN010000000
VERSION     DAPGRN010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.152"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..506
                     /locus_tag="RHW33_004142"
     CDS             <1..506
                     /locus_tag="RHW33_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDV1647593.1"
                     /translation="LFEGVEKKQIEALKIQLQDIKNFKAYYDSELNKFASDFRNFSGA
                     FAINWEDLLKNYQEIRASIKDYDDLLREIKELTIKRDKSVDDKRTLFDNKRDKWDSQE
                     VQDEVAALNQKIADDDNKIRSNLNIVQNFRRENQYKANKKISDAMRNILDWFERYPDI
                     VKNITQA"
     gene            complement(643..1308)
                     /gene="yieH"
                     /locus_tag="RHW33_004143"
     CDS             complement(643..1308)
                     /gene="yieH"
                     /locus_tag="RHW33_004143"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV1647594.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGINVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLKMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTELAQLPALWKARGWN
                     ITR"
     gene            1475..2812
                     /gene="adeP"
                     /locus_tag="RHW33_004144"
     CDS             1475..2812
                     /gene="adeP"
                     /locus_tag="RHW33_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV1647595.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2866..3432)
                     /gene="yieF"
                     /locus_tag="RHW33_004145"
                     /gene_synonym="chrR"
     CDS             complement(2866..3432)
                     /gene="yieF"
                     /locus_tag="RHW33_004145"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV1647596.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDQQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3454..4203)
                     /locus_tag="RHW33_004146"
     CDS             complement(3454..4203)
                     /locus_tag="RHW33_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV1647597.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTMKAFSPN"
     gene            complement(4360..5319)
                     /gene="yidZ"
                     /locus_tag="RHW33_004147"
     CDS             complement(4360..5319)
                     /gene="yidZ"
                     /locus_tag="RHW33_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV1647598.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLTPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEKWDLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDVSQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            complement(5294..6469)
                     /gene="mdtL"
                     /locus_tag="RHW33_004148"
     CDS             complement(5294..6469)
                     /gene="mdtL"
                     /locus_tag="RHW33_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV1647599.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAIMGIAVLMLSLFILKETRPAAPAASDKSRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQM
                     LFLAAGITLAVSPSHAISLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(6601..7848)
                     /gene="tnaB"
                     /locus_tag="RHW33_004149"
     CDS             complement(6601..7848)
                     /gene="tnaB"
                     /locus_tag="RHW33_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV1647600.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSSTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            complement(7939..9354)
                     /gene="tnaA"
                     /locus_tag="RHW33_004150"
     CDS             complement(7939..9354)
                     /gene="tnaA"
                     /locus_tag="RHW33_004150"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV1647601.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            complement(9575..9649)
                     /gene="tnaC"
                     /locus_tag="RHW33_004151"
     CDS             complement(9575..9649)
                     /gene="tnaC"
                     /locus_tag="RHW33_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HDV1647602.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
BASE COUNT         2516 a         2362 c         2386 g         2393 t
ORIGIN      
        1 ccctttttga aggtgttgaa aaaaaacaaa ttgaagcgct aaaaatccaa ctgcaagata
       61 taaaaaattt caaagcatat tacgactcag aactcaataa gtttgcttcc gattttcgaa
      121 atttttccgg tgcatttgca attaactggg aggatttact aaaaaactat caggaaataa
      181 gagcttccat taaagattac gatgacttat tgcgagaaat taaagagtta accataaagc
      241 gtgataaatc agtagatgat aaaaggactt tatttgataa taaaagggac aagtgggata
      301 gtcaagaagt acaggatgaa gtagcggcat taaatcagaa gattgctgac gatgacaata
      361 agatacgttc taaccttaat attgttcaaa attttagacg cgagaatcaa tataaagcta
      421 ataaaaaaat cagtgacgcg atgcgtaata ttctggattg gttcgaacgg tatcccgata
      481 ttgtgaaaaa tataacccag gcttaaccat acagagggtg tgcgactgga cgataaacga
      541 cataaattat ttatagggaa tagacgctta ctcatcagac acctggtatt ttttatctca
      601 aaccagcgtg actgatgtgt aagcgttgaa gtaaggaggc gattatcgcg ttatattcca
      661 gccccgtgcc ttccagagtg caggcagctg cgccagctcg gtaaaggtgg tgactttcgg
      721 gtgaacgatc ggtttattgt gcgggtcagc gcagaagtaa aacacttcca ttccggcatc
      781 tatccctgat tgcgcgccgg cgctggagtc atcgaccaga atgcagtttt caacgttcac
      841 attcatcgct tttgccgcat ggaacattaa tgccggatcg ggcttccaac gctggatatc
      901 gtagccgctg aacagttttt ccgggaagta gtgcagcatc ttgagcttac ccagcgaatg
      961 ctgcattttg ctgaccggac cgttagagac gacgcacatc ggcacgttaa tgctcgccag
     1021 caattcgttc gctcctgcga tgtcctcaag ttcggtatca aacagacgtg ctacttctgc
     1081 gcggtaaacg tgctcggcat cggcctggac catcgtcacg ccctgttctt tactgatgat
     1141 gtcgatgatt tcgtagagtt ttacgccttt aaaacgggta aaaatctcct ccaggtcaac
     1201 gttaatgcca aattccctga acatcgtgac ataggcgcga gagcaaatga cctcgctatc
     1261 gaccagcgta ccgtcgcagt cgaaaaatac cgcttctatc tgggacatgt ctttccttta
     1321 tcgttagttt aacgttttcg tcatgccaat tcgcatgacg caatcgttgc cgtataagca
     1381 aatacaatga aaaaaagtta tcttcagccg tccatattgt ctcattttgg tatacgatag
     1441 cgacggattt tccctccttg ttcggaaata gataatgagt caacaacaca caacccaggc
     1501 ttctggccag gggatgctgg aacgcgtgtt taaactgcgc gaacatggca cgacggcacg
     1561 gaccgaagtg atcgccggtt ttaccacctt cctgacgatg gtttacatcg tttttgttaa
     1621 cccgcaaatt cttggcgttg ctggcatgga taccagcgcc gtcttcgtca ctacctgtct
     1681 gattgctgca ttcggcagta ttatgatggg actgtttgct aacctgccag ttgcactggc
     1741 acccgctatg ggcctgaatg cgttcttcgc ttttgtggtt gtgcaggcga tgggcttgcc
     1801 gtggcaagtc gggatgggcg caatcttctg gggcgcgatt ggtctgctgt tactgacgat
     1861 tttccgcgtt cgctactgga tgattgccaa cattccggtc agtctgcgtg tgggcattac
     1921 cagcggtatc ggtctgttta ttggcatgat ggggctgaaa aatgcaggtg tgattgtcgc
     1981 taacccggaa acgctggtga gcatcggtaa tctgacttct cacagcgtgc tgctggggat
     2041 cctcggcttc ttcatcattg ctattctggc ctcacgcaac attcacgcag cggtgctggt
     2101 ttctatcgtg gtgacaacgc tgctgggctg gatgctgggt gatgtgcact acaatggcat
     2161 cgtttctgcg ccgccgagcg taatgactgt cgtgggtcat gtagatttag ccgggtcgtt
     2221 taacctcggg ctggcagggg tgattttctc tttcatgctg gtcaacctgt ttgactcctc
     2281 cggtacgttg attggcgtga ccgataaagc aggtctggca gatgagaagg ggaaattccc
     2341 gcgcatgaag caggcgctgt atgtcgacag tatctcttcc gtgaccggtt cgtttatcgg
     2401 tacttcttcc gttacggctt atattgagtc ctcttccggc gtatcggttg gcggtcgtac
     2461 cggtctgacg gcagtggttg ttggtctgct gttcctgctg gttatctttc tgtcgccgct
     2521 ggcgggaatg gtgccaggct acgctgcagc tggcgcgctg atttacgttg gcgtgttgat
     2581 gacctcaagt cttgctcgcg tgaactggca ggatcttact gaatctgttc cggcgtttat
     2641 taccgctgtg atgatgccgt tcagcttctc gattaccgaa ggtattgcgc tgggctttat
     2701 ctcctactgc gtgatgaaga ttggtaccgg gcgtctccgt gaccttagcc cgtgcgtaat
     2761 catcgttgcg ctgctgttta tcctgaagat tgtatttatc gacgctcatt aaaatcgata
     2821 gcgcattaaa aacccgccat gcatgattgc tggcgggttt tttatttaga tcttaactcg
     2881 ctgaataaac tcaccaaatg cggtcaattg cccggtcagg tggtccagcg taccctgatc
     2941 aatcacttct ccggtttgct gatcaacttt attctgaatc acgccgccca taaattccgg
     3001 cttgttcatc accattgcat cgaggaaaac cagaatctgg cgcaggtgat actgacagcg
     3061 cgcgccgcca atcacgccca ttgagctggt ctgaatcaac accggtttac cggccagcgg
     3121 ttgatccggc aggcgggaaa gccagtcgat ggcatttttc agcccacccg gtaccgagta
     3181 gttatattcc ggcgtgacga tcaccacacc gtcagcctga cggatctgtt ccgccagagc
     3241 ttcaaccgtt gctggaaaac cttcttcctg ctgtacgtca gcgtcataca ggggaatgtc
     3301 ggcaatggaa ggtaacgcat tgacttccat gctcgccgga gcaattttcg gcagggtacg
     3361 tgcaaccatg ccattaaatg agcctttgcg caggctcccc agtaacgtaa ccacctgcaa
     3421 tttttcagac ataatgactc ctgtggttgt ctatcaattt ggcgaaaaag ccttcatggt
     3481 agagagctgg gcaaaacgca tcatccggct ggtaggattg ttggcgcggc tgtgaatatc
     3541 cggcaggctt ttccagccgt gtttgccatc taactcccag acactgagct tttcaatcgt
     3601 gggcgtgacc gccacggacc acaccagcac gtcctccgcg tcgcataacg cttctacatg
     3661 atttacatga gcacatttca ggcgtccggc aatgggcagc agttgcagat cgcgcggatc
     3721 gtcattcaaa acatcaccgg ttagttttag cacgcttcgg cgcagcgtga tgagctgcgc
     3781 ccgcgcttcg tttggatcat tttgtttact gatccatagc gattcattgc tggaaaaggt
     3841 gtggttatcg ggcgcgtgtg ggctatgaaa cccgcgcgtc gcacgctgca gttccatatc
     3901 gaggccacat tcaccttcgg ttgttaacgc cacgccgacc atattcccgg cataggaaat
     3961 agaaaacgaa ggcaaatttt tatcactgaa aaccggttta cctttcggca gggtgacgat
     4021 ttccggcaat tcgccaatgc catacagcat gaacatcagt tctgcgagta aacctctgga
     4081 cgccagaaaa cggctttgac gatgaggggg aatgtttcgg gcttcttgat gacactggga
     4141 ggggagacga acagaaatca gatgtccttc cgttaaaatc cctcgggcaa agtgagtcgc
     4201 catttttcgc tccatgctta atggtccatt atgaatacaa gagtaatcat ttactggaat
     4261 gatggacaga ctaaagcggc taaaagttcg attcagacaa gcttattaca acttttttac
     4321 attttattgt gaaatcgggt cgcgtttcat acgattcggt tatgccatcg acgcgtaaag
     4381 atttttaatg gtttcccgta accagacgat cttcggatta tggctgttcc gtttatgcca
     4441 cagcagggta aaagggactt ccagcttttt ttgctggctt acgtcaaacg ggagaggaag
     4501 agcaaccaac ggcagttggt ggagttgatt gtagtactgg cagtagcgcg gcgcggtcgc
     4561 caggagcaga ttatcgggtt gcgctgccat aaatagtgac tgctcgaatt ccggcagact
     4621 cattgcaatc gtacgttcgc gccccagctc ctgtaacaca ttgtccagcg cccaggtatc
     4681 gctctgttcc cagcaaatgc tgatatgcgg ataacgtaaa aaggtatcga gatcccactt
     4741 ttcatgcagt gccggatgat ctttgcgtaa ccagacgcag ggcacatcac taaacaacac
     4801 ttcataatca atggctaacg gtagcgagct taacagctcc cgcgagcgag gatggctttc
     4861 gcgaccggaa aaaccaatat ccacttcacc acgagtaatg gcatctaagg aatcgtaatc
     4921 ccagttacgt aatttgatgg tcgcctgcgg ataacgttgg tagatccgtt tcgacagcgc
     4981 attaagcatg atcatcatta gcggtgattc tgccgccagc tcaaacttca ggccgcgcgg
     5041 tgtctggtga tgcggtttat ccagcagaag gttgctcatt tgcatccatt ccgccagatt
     5101 ttgctccatg ctgaccatca gcggcgtggg cgtcagaccc agcggtgagt tcacaaagag
     5161 cgggtcgtca aaccacgctc ttaactttgc cagcgactta ctcaccgccg aaggtgtcac
     5221 gttcatccgc ttcgccgctt tggttacgct gcgctcctgc atcagcagtt gcagacagag
     5281 cagcagattg agatcaagcg tggtgatgga tttcttcatg agcggcaacg gggcgtccag
     5341 gcgcgacgaa cataatcagc aacaggctca ctatgctaca ggcaatcaga atcccgatca
     5401 gcatattcca tgcgccgata ccaaccactg ctgccagcca aatccacagt gacgaaccgc
     5461 aaacctgcgc aatacctaag gtcgagctgg ctacgcccgc gcgtaatgaa aacggcccta
     5521 acgcctgact catcgccaca ccaaaaccaa ccgagaaacc ggcgcaaatc agcgtgatgc
     5581 caaacagaga aatcgcatgg gaaggtgaaa cggcaagggt gatccccgcc gccaggaata
     5641 acatctgcga agtgatcatc aacgtacgtg gcttaaagat tcccagcgca aatggcgtcg
     5701 agaatgaaac ggtcatgctg acgccagcgg tcaacgccat aatggtggcg tattctccgc
     5761 gctcaaaacc catgatttcc atcagcaata ctggcgacgt gttgacgaag gtgaggatca
     5821 ccgaaacgct gagggtggtg ataacaacac ggctgaggaa aaaccgatta agcagcgact
     5881 cgctattttc tcgtgatttg tccgaagctg cgggggcggc agggcgcgtt tcttttaaaa
     5941 taaacaaaga caacatcagt accgcgatgc ccattattgc catcgtccag aacagactct
     6001 gccacgggaa tttaagcata atcagatgac cgagcaccgg cgctaacacc ggaatgatgc
     6061 aggtaatgcc gttgagtagc gacagcactt ttgcccgacg tcgatcatcc agcgtgtcgc
     6121 gcaaaatggc gaatgccacc acgtaacaac agcccgcgcc caacccctgt agaaagcgcc
     6181 ctgcaagaaa taacgtgctg gtttcagcca gtgaacagaa caccgaggcg ataataaata
     6241 gcgccgcgcc ggggatggcg accggttttc tccctgaacg atcggccact ttaccggcaa
     6301 ataacatcgc agctgccata cctgccagat aaacggagaa cgcaatatgt aactgcgctt
     6361 cgctggcatt gagatcggcg gcgatgcgcg gtaaaccaac gaggtacata tcaatcccag
     6421 cgggataaag taaaaccagg gcaaaactac aaatcaaaaa gcgggacatg ggctaaagcg
     6481 tcctgatagt aaggtgacgc aaagcatacg tggtgaagga ggatcaggcg agttgccatt
     6541 tggacataag tgatttcctg agaggcaaga agccagcgaa tggctggctt cttgaaggat
     6601 ttagccaaat ttaggtaaca cgttaaagac gttgccgaac cagcacaaaa ttacggttat
     6661 accaaagaga atgacaatcg ccggaataag attgccgccc cagaccgtga acatctgatt
     6721 gggaaacttt ttgcgagctt tgattgccag caccgcgggg ataatgaccg cccagatagt
     6781 ggcgcacagt ccggcaccgc cgatcccgta aataaagccg ttcgggaaga tcagatacaa
     6841 caacgcgggt ggcaggaagg ttaacagcac ggttttgaaa cgcccgccgt gggagttatc
     6901 aatcttaaac aggtccgcca gataatcgaa caatcccagc gtgacaccaa aaaacgagct
     6961 ggcgacagct aagttggaga acaccagcag gcaaaactcg ataatgccgt gctgtttggt
     7021 gccgaggaac gatttcacca gtgaatcaac gttgccgcct gaggaaataa tcgccttaaa
     7081 gctttctcgc ggaatattcc ccatggtgca atagagccag aagagataaa tcaccagcgc
     7141 cagcagcgaa ccgaagacca cgcttttgat tagcttatct ttgcgtttgc catagcaaat
     7201 aatcaggctg ggaatattgc cgtggaaacc aaatgacgcc agacacaccg gcaaagccat
     7261 aaagatatac gggaagtaag acgttcccgc agtggagctg gtggcatcgc gcagaatgga
     7321 gtaatcgacc tggaagaaga aagaaccaaa cacgatcaca aaggagataa tcttcagccc
     7381 gaggaacaat gaggtaatac gactggcggc taacgagctt atccacaata cgctggcaac
     7441 gaaaatggct gtgcagatcc cgacaatacg tggattagcg tgataaccca aattcattga
     7501 tatcgtttca ctaatgatcg caccattagc agagatatag gcataagtga ggatatagag
     7561 aacgaaggca acggtaatac cgctgataat gttccaggtg ttaccgatta aatctttggt
     7621 gatggtgtta aaactggagc cgacgggata atttaaattt gcttctaata acaataaccc
     7681 ggaatgaagc attgaaaacc aggcaatgat aaggataaag gcaccccaga aaaaccaggc
     7741 accggcaaga tcaacaggta aagcaaacat acctccacca attactgtac ctgctataac
     7801 cataacaccc caaaatgcag agtgtttttt ttcagcttga tcagtcatga tgccaccttt
     7861 agaggaaggc tatttttgtt attgaggatg tagggtaaga gagtggctaa catccttata
     7921 gccactctgt agtgtcaatt aaacttcttt cagttttgcg gtgaagtgac gcaatacttt
     7981 cggttcgtag gtaaaggtta accctttaat attcgccgcg ttctctttca catgtttaaa
     8041 ggcttcaata atgaagtcca tatgtgtttg agtatatgtt gcgcgcggaa tggttaaacg
     8101 cagtagttct gccgggcatg gcagttgttt accggttttc ggatcgcggc ctaacaggaa
     8161 agagccaatt tctaccgcac ggataccggc gactttatac agctcgcagg ccagcgcctg
     8221 tgccgggaat tgatcagccg ggatatgcgg caacagttta ccggcatcaa cgaatgccgc
     8281 gtgaccgccc gcctgctggc agacaacgcc aatctcttcc agaccatcga ccagatactg
     8341 cacctgcgca atacgataag ccagccagtc cagattcatg ccgtcataca gacctaccgc
     8401 cagacgctcc atcgcgccgc cttccaggcc gccatacgtc gggaagcctt cctgaactac
     8461 gcaaagggtt ctgcactcgg tgtacacatc aaagaaactg tcgtctttca tgcacagcaa
     8521 gccgcccatc ggcaccatcg catctttctt ggcggacatc gccagcatat cggcatattt
     8581 gtaggtttcg cgggtgatct gctcgatggt ccagtctttg tattctgctt cacgctgctt
     8641 gatgaaatag gcgttttcag caaagcgtgc agagtccatt accaccggaa tatcgtattt
     8701 cttcgcgatg ctgtacatcg cttttaagtt tgccagtgaa accggctgac cgcctgcgga
     8761 gttactggtg atggttgcaa cgatatacgg cacgttattc ggaccaactt cttcaatacc
     8821 gcgttctaat ccctcaaggt caaagttgcc tttaaagtcg taacgcacgc ccgtatcgaa
     8881 ggcttctttg atatagacgt tacgcacggt acagccgtta atctggctat gtccctgcgt
     8941 ggtatcaaag aaatagttgg agaacgccac cattttgctg cgatccaggc ctttttcctg
     9001 ctcgcgcttt ttaatcagta ccggaatata gatttgctct gcaccacggc cctggtgagt
     9061 cggaatggtg tattgataac caaagatatt tttcactgac tcggctaacg catagtagct
     9121 gcggctgccg ctgtaagctt cgtcgccgcg catcatcgcg gcctgcatac tctgcgtcac
     9181 cgccccggtg ccgctgtcgg tcagtaaatc gataaacaca tcttcgctat ccagcaggaa
     9241 cgggttcata ccggatttaa taattgcttc ttcacgataa gcgcgagtgg tacgttttac
     9301 aggctcaata acacgaatgc ggaacggttc agggagatgt ttaaagtttt ccattacata
     9361 atccttcatt tattttaatt acagtgatcc ctgtgaatat tacatttgca atatccatta
     9421 attcgccatg tatatatgca agcaccatca tttactaaca caagtaaata aacgagagat
     9481 ggcctttgca aaaggcaaat ttaagatata gcaaacaaat agatcacatt gaatctaatc
     9541 ggtttggctc tggtgatggc tacagaaggg caaatcaagg gcggtgatcg acaatcttgt
     9601 tgtcaatatt gaaccatttt gaggtcacac atatatgtaa gatattcata atgcact
//
LOCUS       DAPGRN010000151         9595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPGRN010000151 DAPGRN010000000
VERSION     DAPGRN010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.153"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(64..822)
                     /locus_tag="RHW33_004152"
     CDS             complement(64..822)
                     /locus_tag="RHW33_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV1647603.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="RHW33_004153"
     CDS             complement(830..1933)
                     /locus_tag="RHW33_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV1647604.1"
                     /translation="MTKVLLSHPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFALERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /locus_tag="RHW33_004154"
     CDS             complement(1943..3124)
                     /locus_tag="RHW33_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV1647605.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="RHW33_004155"
     CDS             complement(3192..4217)
                     /locus_tag="RHW33_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV1647606.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="RHW33_004156"
     CDS             complement(4648..4869)
                     /locus_tag="RHW33_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647607.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="RHW33_004157"
     CDS             complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="RHW33_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV1647608.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="RHW33_004158"
     CDS             complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="RHW33_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV1647609.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2556 a         2483 c         2289 g         2267 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt atgcttttcg aacgaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgacttag gatgcgcaaa
      121 aaactcatcc ggtgcagctt gctccactat ttcgccacga tccataaaaa tcacccggtc
      181 agcgacggtt cgcgcaaacc ccatctcatg ggttacacac aacattgtca tacccgattg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgatgt
      301 gggctcatca aacaacataa ttttcggctt catacacagc gagcgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accacgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat caatttgtcg atactggtgc
     1261 cttctgccat aaacaaaggc agcatgaccg aagacataaa cagaacggta atcagcggca
     1321 cgccgcgcca gaattcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca actgatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agagcaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gattgacaca aggccaatca
     1621 gtaatgccag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgaggga tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt ccacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tgattcagca cggtgccggc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggtggaat aatcacccgc aatgcttggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggaaagct
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagccc aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcacgaac cgtagggttg gcaaaggaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgctcgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc aaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccaa cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcatttag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ccgggttgct taatttgatg
     3601 cgcagggcat acagttggga ttgatccgaa gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcactggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcgacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccagt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacctcc cctgactgta aagcggtgaa gcgttctttt
     3961 gcagtgagcg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaatat caacatcaat acctgaaaat ttaccgtcag catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccatgttttg
     4321 cgcacgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4381 gcaatggtgc gaaaatgaaa catacgccac attttggcgc aaaatattta cgcgtgtttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg cgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4561 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     4621 gagcgcgaaa ctccgcgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gctgctgctc cgccagcaat agccccagcc gtttcccagt tatcctgagt
     4741 tgtgcctttc atacaggtat tggtgtgaac cagacgcccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcaccggag gttataaatc ttacggaatg aataatgaca
     4981 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagtt tttaaagagt
     5041 gagtggaaga atatcttatt aatttttatg ggtttcactg gaaataataa aggcgcccga
     5101 aagcgccttt atgtttctga tttatccttt aaagcaacgg cggatcacca caaagaacac
     5161 tggtacgaag aagattgcca gcaacgttgc agagaccatt ccgcccatta ccccgatacc
     5221 cactgcgttc tgcgcgccac tgccggcacc gttactgata gccagcggta atacgccgag
     5281 aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg ccattagtgt
     5341 cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact caacaatcaa
     5401 aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa agtagacgtc
     5461 atttttttga ttaaagagtg tcgccgccag cagcacgccg acaatcccta acggcacgac
     5521 caacataacc gagacaggaa ttgaccagct ttcatagagt gcagcaaggc acaagaaaac
     5581 aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc gttcctgata
     5641 cgacataccc gtccagtcat aaccaatgcc cgcaggtaat tttgacgcta ggttttccat
     5701 caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa tctccattga
     5761 cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg aagtggtaaa
     5821 ggcagagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat cgacatcttc
     5881 tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacgc gaccacggtc
     5941 gatgaagtcg ttaacgtaag tcccacccag cgccgttgaa atggtctgat tgatgtcaga
     6001 aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa actgcgcggt
     6061 gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg ccgccatacc
     6121 aagcaactgg ttacgggcct gggttagggc atcgtgaccc agcccagcct gatcaattaa
     6181 ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat tgaatggaat
     6241 gacaaaaccg tcgcggatct tgcccaactc cattttggca cgatggatta ccgcttccgc
     6301 actgttttcg tcaccactac gctcttccca cggtttcaga ctgacgaagg ccataccggc
     6361 gttttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt caacgtttgc
     6421 tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg tccgctcttg
     6481 cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct cttcaggtaa
     6541 gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca gcgcatagat
     6601 cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt agtggttaac
     6661 gctatgatcg aaggtggtat taaaccagcc gaagaaacca cccttatttt cgtgatgctc
     6721 agctgaggtg ggtttgagca gtgttgcaca taacgcaggg gtaagaatca atgccaccag
     6781 aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa ttgccccagt
     6841 agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg cgatacccac
     6901 cagtgcgccc tggatttgcg acattgattt ttccgtcgct tcttttggcg ggagcttatc
     6961 ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga cgagcagccc
     7021 gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa aagcggcgag
     7081 gatggcaaac gtccctaaca acaccacggg taccgcaatg gtggggatca gcgttgctcg
     7141 catattctgc aagaacagat acatcaccag gaacaccagc ataatagctt cgaacagtgt
     7201 ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag ggtagagaac
     7261 cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa tggctttcgc
     7321 ggtatcgaga gcgttcgcac cggttgccag cttaataccc aggcccgccg ccggttttcc
     7381 gttgatgcga gcgataacgt tatagttttc accgccaagt tcaacccgtg cgacatcttt
     7441 cagacgcacc accgagccgt cactgtttac gcgcagggtc actttgccga attcttccgg
     7501 attttttaac cgcgtctgag caataatcga ggcattcaac tgttgccctg gtaacgctgg
     7561 cgttccgccc aactgtccgg cagcgatctg atcgttctgt accttcaact ggttaatcac
     7621 atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga tacgcatcgc
     7681 atactgtgcg ccgaaaagct gtacgtcacc gacgccattc agacggctaa gcgtatcttt
     7741 aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt tatcagagac
     7801 aaagcccgcc accatcaaat agctgctact ggacttttca acactgatcc cctgctgctg
     7861 aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct gcacttgcgc
     7921 gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac cggcggaatc
     7981 gctggtggag gacatataca tcaggttatc gataccattc atattctgtt cgataacctg
     8041 cgtcaccgta tcctgcacgg tctgcgcatc agcacccgga tagtttgctg aaacagaaac
     8101 cgcaggcggt gcaatggttg gatactgagc gacgggcaat tgtaggatcg ccagtgcgcc
     8161 cgccatcatc agaataatgg ccagcaccca tgcaaatatt ggtcgtcgaa taaaaaagtt
     8221 tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt agtggcttta
     8281 acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc gccagatttc
     8341 agtccttcac taatcaacca tttatcgcca atcgcctgac tggcaacgac agggcgcgct
     8401 tcaacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg tgtgcggcta
     8461 acgccttgtt gcgggataag aatggcgtca ggttggacgc cttcatcaat ccgtgcacgc
     8521 acaaacatac ccggcaaaag tgtatgttgc gggttaggga agacagcacg tagggttatg
     8581 gagccggtgc tttcatcaac ggtcacatcg gagaattgca gcgtaccttt caggggatag
     8641 gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt atgcaaattt
     8701 ccttgctcta ctgattgctt cagcctcata aaatcgttgc tggattgggt cacatcaacg
     8761 tagataggat cgagctgctg gacagtcgcc agttcagtcg tttgcccatt agtgacaaga
     8821 gcgccttcgg tcacagtcga tttgccgata cgtccgctaa ttggcgcagt cactttggta
     8881 taagcaagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac ggcggcatcg
     8941 gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta tttcgtaccc
     9001 acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc
     9061 gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc gatctggtac
     9121 agggactggc ctgcttgcac atcgctgcct tcagtgaaat tgcgattcag tacgatcccg
     9181 ctaacctgtg ggcgaacttc ggctatacga taagcattgg tgcggcctgg taattcagtc
     9241 ttaacttcta acggggccgt ttttacaata tgaacggtaa cctgcggttc accgacgtga
     9301 gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat cagaataaag
     9361 gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc
     9421 gcgttattta ccccaaaagg caacgcgtta actcgcagaa agaaaaatac agttcgctat
     9481 cctacaaatt atcattcgtc gatgtaagga atagttatga atacaggcgt ctcaaggcac
     9541 ataaacacaa aaaaagatta atattctact gttttatttt gacgcgggtt gaaag
//
LOCUS       DAPGRN010000152         9546 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.154, whole genome shotgun sequence.
ACCESSION   DAPGRN010000152 DAPGRN010000000
VERSION     DAPGRN010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.154"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(77..877)
                     /locus_tag="RHW33_004159"
     CDS             complement(77..877)
                     /locus_tag="RHW33_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647610.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVARAENMNWMQALRFVAAKGHQKAIIV
                     YQDMLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     SRVIFPPAGITKTSNKELSNDHVTEAQLFNLKQKTYVSMVKDLVSRFQ"
     gene            complement(1205..1281)
                     /locus_tag="RHW33_004160"
     tRNA            complement(1205..1281)
                     /locus_tag="RHW33_004160"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1245..1247),aa:Asp,seq:gtc)
     gene            complement(1414..2145)
                     /gene="dnaQ"
                     /locus_tag="RHW33_004161"
     CDS             complement(1414..2145)
                     /gene="dnaQ"
                     /locus_tag="RHW33_004161"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV1647611.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            2210..2677
                     /gene="rnhA"
                     /locus_tag="RHW33_004162"
     CDS             2210..2677
                     /gene="rnhA"
                     /locus_tag="RHW33_004162"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV1647612.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2674..3396)
                     /locus_tag="RHW33_004163"
     CDS             complement(2674..3396)
                     /locus_tag="RHW33_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1647613.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3430..4185
                     /gene="gloB"
                     /locus_tag="RHW33_004164"
     CDS             3430..4185
                     /gene="gloB"
                     /locus_tag="RHW33_004164"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV1647614.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTSSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4257..5615
                     /gene="mltD"
                     /locus_tag="RHW33_004165"
     CDS             4257..5615
                     /gene="mltD"
                     /locus_tag="RHW33_004165"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV1647615.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTLLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5663..6433)
                     /locus_tag="RHW33_004166"
     CDS             complement(5663..6433)
                     /locus_tag="RHW33_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1647616.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWGVRMRTPEALVG
                     AIRIYQQSASTEVRTYFSLQNDGSFTSDIIMVEAHKAA"
     gene            complement(6511..7311)
                     /locus_tag="RHW33_004167"
     CDS             complement(6511..7311)
                     /locus_tag="RHW33_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV1647617.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7552..8466
                     /locus_tag="RHW33_004168"
     CDS             7552..8466
                     /locus_tag="RHW33_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1647618.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVRET"
     gene            complement(8463..9266)
                     /gene="dkgB"
                     /locus_tag="RHW33_004169"
     CDS             complement(8463..9266)
                     /gene="dkgB"
                     /locus_tag="RHW33_004169"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV1647619.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNIQLDAEAKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(9429..9505)
                     /locus_tag="RHW33_004170"
     tRNA            complement(9429..9505)
                     /locus_tag="RHW33_004170"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9469..9471),aa:Asp,seq:gtc)
BASE COUNT         2561 a         2270 c         2256 g         2459 t
ORIGIN      
        1 catatttaca ttataaaaat actcaactcc tgtttgctgc caccctaaac tacaggcggc
       61 agcataacaa taacgattac tggaatcgac tcaccaagtc tttcaccatc gatacatatg
      121 ttttttgttt caaattaaac agttgcgctt ccgtaacatg gtcatttgat agttctttat
      181 tgctcgtttt tgttattcca gccggcggaa aaattactct cgattcataa ttcaccggcg
      241 accaggtagc ccactcacct gttgccacat ccaccagcgt gaaacgtacc aggtagcgca
      301 gggagatagc gtcagtgagt ttttcatttt tatagtcgga ccatactgtt gatttcagcg
      361 cagagtcata ttttcctgtc tgcaacatat cctggtaaac aataatcgct ttctgatgtc
      421 cttttgcagc cacaaaacgc agtgcctgca tccagttcat attctcagca cgggcaacat
      481 cctcgttctc atttttatct ttgtttttgt tacaagtcag aggcttttgc ctgtccggga
      541 taccagagaa tgtggaaaca gtataatatt ttcgcatctc ctcctgcata atggtttccg
      601 gtgcgcggtt gccggattga acaagtatta caggagaatt aaggggaacg cggaactcat
      661 ctccatcaag cgctgcttga atatcctctt cggatacggc cagagtggtt tcattaccga
      721 ataaatcttt gtctgtcagt tgctggggag tgtttttatt aatattactt tgtgtgactt
      781 gagctttttt cttcttttca tcaatagaag attcagaggc acatcctgca agcagcgaga
      841 ataacacaca tatgcaacaa agcttttttg aattcataat tggacactcc ctcgcctgtc
      901 ataatgatta gaatatttag cattgataac ggactctaat tatatacccc acttaaataa
      961 taacaaacaa aatcaatttg aaatatacat tcatttaatc aatataaatt tattctctgg
     1021 aaatatattt aagctgaatg gttcgatatc attattgaat tttaatgaat gagacaacaa
     1081 gcgagaatca cgcacttacg ccagagtaaa atcattgaac agagaaatgg tggaattcaa
     1141 atacaaaaaa accgcaaaat taaaaatctt gcggttctct gaactcattt tcatgagtga
     1201 atattggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
     1261 aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa tattacggat
     1321 tgcctcaccc gtcgtcaatg gtttttctca tcttttgaat cgtttgctgc aaaaatcgcc
     1381 caagtcgcta tttttagcgc ctttcacagg tatttatgct cgccagaggc agcttccgcc
     1441 tttcttctgc accagatcga gacgggcttc atgtgctgca agctcttcat ctgtcgcaaa
     1501 aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt caccttgttg
     1561 ctgttgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg tcatcgccag
     1621 ataaacttcc gcaaggatct gggcatcgag taatgccccg tgtagcgttc gtttactgtt
     1681 atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag gaaacatttt
     1741 cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg gaatatcgcg
     1801 cttaagcaac gagaactcgt agtccataaa gccgatatcg aacgctgcgt tatggatcac
     1861 caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa acgtgggctt
     1921 atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat ccaccagccg
     1981 atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca ccacttcaac
     2041 ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt tcataccggt
     2101 ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg tcatttatgt
     2161 cagacttgtc gttttacagt tcgattcaat tacaggaagt ctaccagaga tgcttaaaca
     2221 ggtagaaatt ttcaccgatg gttcgtgtct gggtaatcct ggacctgggg gttacggcgc
     2281 tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc gcaccaccaa
     2341 caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag aacattgcga
     2401 agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt ggatccataa
     2461 ctggaaaaaa cgtggctgga aaacagcaga caaaaaacca gttaaaaatg tcgatctctg
     2521 gcaacgtctt gatgctgcgc tggggcagca tcaaatcaaa tgggaatggg ttaaaggcca
     2581 tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgccgcgg cgatgaatcc
     2641 cacactggaa gatacgggct accaagtcga agtttaagcc tgtggtttac gacattgccg
     2701 ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca tcggatttag
     2761 cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa gcgcaggaat
     2821 atgcgcattc aaaagttttc ctccgtgttt gttccacggg agaacgtgga aacggctggc
     2881 gtgcagcact tcaaaattca acaaagagag ccagtccagc tgccgcatca gagtaaacat
     2941 ccggctgtta tagggcgagg ttttgcgcaa taccggcaca agtttgcgta atcccattaa
     3001 actaatggga ttgaagccac taatgaccag ccagccatca tcaatcaata cccgatcggc
     3061 ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca gtagacaaac
     3121 atcaacggat ttatcggcaa aaggaagatg aagtgggtcc gcctgcacct ggacaggcat
     3181 tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat tgatttctgc
     3241 gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc acgggttgag
     3301 ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc agcgatcagg
     3361 agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt tcacactgag
     3421 aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat ctgggttttg
     3481 aatgatgagg caggtcgctg cctgattgtc gatcccggag acgcagagcc agtattaaac
     3541 gccattaccg ccaataactg gcaaccggag gccatatttc tcacccacca tcaccacgat
     3601 cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt gtatggtcca
     3661 caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac tgccttcgtt
     3721 ttagggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca tatctgttac
     3781 ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg tggtcggttg
     3841 tttgaaggga catcatcaca aatgtatcaa tcacttaaaa agttaagtgc gttacctgac
     3901 gatacattgg tatgttgtgc tcatgaatat accttatcaa atatgaagtt tgctttgagt
     3961 attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga gttacgggca
     4021 aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa tgttttttta
     4081 agaacggaag atattgattt aattaatgta attaatgaag aaacattatt gcaacaacct
     4141 gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa ctttcttgtc
     4201 atcggcttcg ttcgccgtta tgatcggtcg tcttttaagc aactattgac acacacatga
     4261 aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt accggtaacg
     4321 tccaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca aagtttacaa
     4381 gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac ttgtgggctt
     4441 tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc gaacagaaac
     4501 agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca gagccgtata
     4561 tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg gtactactac
     4621 ccatagtgga gagcgctttt gatcctcacg caacgtctgg tgccaatgcc gcaggcatct
     4681 ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc aattatgacg
     4741 cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag cgtctgaaca
     4801 aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc gaaggtcggg
     4861 tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc tggtcgttac
     4921 cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt gatattctca
     4981 aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt gctctggcgc
     5041 gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg gggatttccg
     5101 tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc gcaagtggtc
     5161 cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct ctggcttcag
     5221 gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgctgctt aacagccgtg
     5281 tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc ggcgtaagca
     5341 ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca ggccaaagtt
     5401 tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat agcattacgt
     5461 atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc gtgaacatca
     5521 aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat aagctgacgt
     5581 tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa aaaggcaccg
     5641 attcccccgg tgcttttttt atttatgccg ctttatgtgc ttctaccatg atgatatcac
     5701 tggtgaaaga gccatcattc tgcaaggaaa aatacgttct cacctctgtc gatgcgctct
     5761 gttggtaaat gcgaatagcg cctactaacg cttctggcgt gcgcattctc acgccccatg
     5821 aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga ttggcttcat
     5881 tgattaacct caaccactca ccgctggcgt agtttcgtac gtgagaggta tcgcgtaatg
     5941 cttctaccgt ctgtaaccag atgtcgcgca ctgggtgacc cggagacatt acgtccatca
     6001 caatcagcct accgccaggt ttcaatatcc ggttcacttc acgcagtgct gcaccaacat
     6061 catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta tcggcaaatg
     6121 gcagactttc ggcataaccc tggcgggtgg tgatattttt cagttgccgg acttcggcag
     6181 cttgtgccac gacatccagc atttgggcag ataagtcata cgccaccacc gtgctcacgt
     6241 tttgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt acacttgcat
     6301 caggataatc agccagacac accgccaggc gctgtaaatc tcgtccggat gcatgtacgg
     6361 tactggtcag atattcgctg gcttgagagc taaactgctt ttctacatgg tcatggtggg
     6421 attgtattgt cattgcgctg tccttttgtt atttgagtat aaaagggcag cgccaaacgt
     6481 tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg gactgaattc
     6541 aacgagtagc ggattgtgat cggacgcgcg cgtaaccagt acagaagctt cgctgacgtt
     6601 cagaccacgg tagaaaacaa aatcgagcgg gcgaccaaac gcccgacggc gctgatcatc
     6661 ggtaaaacgc acctgacgca gcgacatttc ccgcgcaaag cgatataacg cgttcatcct
     6721 tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt ggtgagctat
     6781 ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac tgaagttgac
     6841 ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga ccgtcaccag
     6901 tgccgacttc gccagacgca aaatgggctc tcgttcacgt aacgggcagc aatacactgg
     6961 atgcgccgcc gaaagggtca ttacgccaga aggatgttgt ggcagcacga aagcgggtac
     7021 ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg ttgtctgcgc
     7081 ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg acaaccattc
     7141 agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt cggtacttaa
     7201 cggttctcca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg gtaagatcct
     7261 ctccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca cttttgactt
     7321 tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt atagggattt
     7381 tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct tacaccggtc
     7441 agaattttgc tcattattga gaacattaaa tcggatgaca tatcacgcat cctgatggtt
     7501 taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa aatgaaagcc
     7561 acgtcggaag aactcgccat ttttgtttcg gtcgtagaaa gcggcagctt tagccgggca
     7621 gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa gctggagatg
     7681 aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac ggaagaaggc
     7741 gagcgttatt tccgtcgcgt acagtcaatt ttgcaggaga tggcagcggc agaatcagaa
     7801 attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc aactccagtg
     7861 gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc ggaagtcact
     7921 ttatcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt ggatgtcgcg
     7981 atacgcgccg gtacgttaac ggattccagc ttacgtgcca ggccgttatt taacagttat
     8041 cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga aacgatcgac
     8101 gatttaaagc aacatgtttg cctgggattc actgaacccg cttccctcaa tacctggccg
     8161 atagcctgta gtgatggaca attacatgag gtgaagtacg gtttgtcatc caatagtggg
     8221 gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc cgactacatg
     8281 atcgacagag aaatcgctcg cggagaattg gtggagttaa tggcagataa agtgttgcca
     8341 gtggaaatgc cattcagtgc cgtctattac agcgaccgtg cggtaagtac gcgcatccgg
     8401 gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc tgtcagagag
     8461 acttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt
     8521 gcggcgatcg cttttttagc ttcggcatca agctgtatat tttgtgcctt aagattactc
     8581 tccaggtttt cacgtttagt agaagaagga attactgagt aaccttcccc catagcccac
     8641 gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc aataacctca
     8701 tctttcaggg cattaccata cgccagcgtc atataggaag taatatggat gccgtgctgt
     8761 ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat ctggttagta
     8821 gcgatgtttt cagcgccaac agcagcaatc gccttttcca tcaatgggat cgtgaaattg
     8881 gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc ctgcataaac
     8941 tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag atcaacataa
     9001 tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc tttgctgaga
     9061 ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca
     9121 atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa
     9181 ccaagttcaa gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagta
     9241 cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg
     9301 aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga ggatttttgc
     9361 gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac tcgaggcttc
     9421 ttaataagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
     9481 ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt tttatgaccg
     9541 gcttac
//
LOCUS       DAPGRN010000153         9364 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPGRN010000153 DAPGRN010000000
VERSION     DAPGRN010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.155"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(33..108)
                     /locus_tag="RHW33_004171"
     tRNA            complement(33..108)
                     /locus_tag="RHW33_004171"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Lys,seq:ttt)
     gene            complement(273..1064)
                     /gene="cpoB"
                     /locus_tag="RHW33_004172"
     CDS             complement(273..1064)
                     /gene="cpoB"
                     /locus_tag="RHW33_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV1647620.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQSFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1074..1595)
                     /gene="pal"
                     /locus_tag="RHW33_004173"
     CDS             complement(1074..1595)
                     /gene="pal"
                     /locus_tag="RHW33_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV1647621.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1630..2922)
                     /gene="tolB"
                     /locus_tag="RHW33_004174"
     CDS             complement(1630..2922)
                     /gene="tolB"
                     /locus_tag="RHW33_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV1647622.1"
                     /translation="MKQALRLAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3055..4320)
                     /gene="tolA"
                     /locus_tag="RHW33_004175"
     CDS             complement(3055..4320)
                     /gene="tolA"
                     /locus_tag="RHW33_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV1647623.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEVDAKAAEDAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4385..4813)
                     /gene="tolR"
                     /locus_tag="RHW33_004176"
     CDS             complement(4385..4813)
                     /gene="tolR"
                     /locus_tag="RHW33_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV1647624.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4817..5509)
                     /gene="tolQ"
                     /locus_tag="RHW33_004177"
     CDS             complement(4817..5509)
                     /gene="tolQ"
                     /locus_tag="RHW33_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV1647625.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5506..5910)
                     /gene="ybgC"
                     /locus_tag="RHW33_004178"
     CDS             complement(5506..5910)
                     /gene="ybgC"
                     /locus_tag="RHW33_004178"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV1647626.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6060..6353)
                     /gene="ybgE"
                     /locus_tag="RHW33_004179"
     CDS             complement(6060..6353)
                     /gene="ybgE"
                     /locus_tag="RHW33_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV1647627.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6353..6466)
                     /gene="cydX"
                     /locus_tag="RHW33_004180"
     CDS             complement(6353..6466)
                     /gene="cydX"
                     /locus_tag="RHW33_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV1647628.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6481..7620)
                     /gene="cydB"
                     /locus_tag="RHW33_004181"
     CDS             complement(6481..7620)
                     /gene="cydB"
                     /locus_tag="RHW33_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV1647629.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7636..9204)
                     /gene="cydA"
                     /locus_tag="RHW33_004182"
     CDS             complement(7636..9204)
                     /gene="cydA"
                     /locus_tag="RHW33_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV1647630.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2296 a         2551 c         2245 g         2272 t
ORIGIN      
        1 actacgctgg atactacaac taactaggtt agtggtgggt cgtgcaggat tcgaacctgc
       61 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccac tttttcgttg
      121 ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt tcagacttga
      181 gcgcaacaaa aatttcgatg tagatcactc aactgcttat gattcgcacg acacgaccag
      241 aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg tttttgcgcc
      301 tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac ggctttcgct
      361 tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc atctgcagcc
      421 tttggtgact tcggatagtt tttcactacc gaagcaaaat agtacgccgc atcatctttt
      481 ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag gtaagttgaa
      541 tcagggtaat ttttgatgaa actctgaaat gccaccattg cgtcatcctg gcgggattta
      601 tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcaccgct tttcaccggc
      661 gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc cgcaccgctt
      721 tgatcaccgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc gatctgcaac
      781 aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc ctgaatctga
      841 ccacgcaggg aatcaatatc ggattgatta tcagagagtt gttgctggag ttgggttaaa
      901 agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc gaccgagcct
      961 gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc tataccaacc
     1021 agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt ctcttagtaa
     1081 accagtaccg cacgacggtt tttggagtat gccgcttcgt catgacccag tactgcaggt
     1141 ttttctttac cgtaagaaac gatggagatc tggtctgcag aaacgccttt accctgcagg
     1201 tacatcttaa cggcgttcgc acgacgttca cccagggaga tgttgtattc cggagtacca
     1261 cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg caggaagttt
     1321 gcatgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc cagatcgaag
     1381 taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc ttcggaagac
     1441 atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat gccttcgctg
     1501 ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac aggcagagca
     1561 atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt actattcaat
     1621 taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat cagttgccgg
     1681 aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc ccatcccctg
     1741 agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt catccaggaa
     1801 cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa tgtgctgctg
     1861 cccaccgttg gagctgacca ttaccataaa tttaccgtcg ctgctgacat ccgcatcctg
     1921 gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat tcactttata
     1981 aacctgtgga cgaccggcct ggtcagaagt aaatgccagg ttctggctgt ccgggaacca
     2041 ggtcggttcg gtattgttac tgcgaccatc agtcacctgg cggatctgac cagaagccaa
     2101 atccattacg tacaggttca ggctaccggt tttcgacaag gcgaatgcca gtttgctgcc
     2161 gtctggcgag aatgcaggcg caccgttgtg acgcgggaat gaagccacct gacgtacagc
     2221 gccgtttgcc agcgtttgga taaccagtgc ggaacgaccg ctttcgaagg tcacataagc
     2281 cagtttagaa ccgtctggtg accacgccgg ggacatcagc ggctgtggtg aacggtgaac
     2341 gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga actgaccgcc
     2401 gttggtctga acaacgtagg caatacgggt acggaacgca cctttaatac cggtcagttt
     2461 ttcaaacact tcatcactgg cggtatgacc agcataacgc agccactgct tgttcacttt
     2521 gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa gttgataagc
     2581 aacattgtaa gaaccatccg gattcggagt gacctgaccg acaactacag cgtcaatacc
     2641 cagtgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct gtggcagacg
     2701 agcgcgatct aacggattaa atttaccgct gttacgcaag tcggcagcaa cgatgccgcc
     2761 aatatcttca ggtgccgcac cagggcccgc ccactggaaa ggaacaactc caataggacg
     2821 accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa ctgatgccca
     2881 cagtatgaga aaaccaaatg ctaatcgtaa tgcctgcttc atcatatctc ccttatctgg
     2941 accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca aaactaccag
     3001 aaccccgtgg caaccggtgc ctgatgttga ccgtcggaac agtcaacatc gcgattacgg
     3061 tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg gtggtttcgg
     3121 gatcttcgca agtttagctg ctgccaacgc agcctgacaa agtgcgggat cgccaccttc
     3181 aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg tacaggtttt
     3241 gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa tctgcccggc
     3301 atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac tcccggcagg
     3361 cgaagcattg ttccctttcg cccctccccc cgttttcggt gcattcttac cagagcttag
     3421 ctcaccgaaa atatcatctg cctctgcggc agcttttgct gcagcggctt tttcagctgc
     3481 tgcttttgca gctgccgctt ttttgtctgc ggcggctttt tctgccgctg ctttcttatc
     3541 agctgctgcc ttttctgcag ccgctttttc tgctgctttt ttgtcggctg cagctttctc
     3601 tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct cagcttctgc
     3661 cttggctttt tcagcagctt cagttgccgc tttctttctt gcttcagctg cagctgcttc
     3721 tgccgcttcc gctttcttct tcagcgccgc agctgctgcc tcggcttttt tctgcgcttc
     3781 ggctgcggct ttggcggctt ctgcttctgc ttttttcttc gcatctgcag ccgctttctt
     3841 cgctgcatct tccgcagctt tagcatccac ttcggccttc gctttagcat ctgccgccgc
     3901 tttcgctgcc gcctcttccg cttgcttctg ctttaactcg gcctgttttg cggcttcttc
     3961 agcctgcttt ttctgttcct gagcagctaa ccgctctttc tcaagttgct tcaggcgttc
     4021 ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgttcct tcatcttgcg
     4081 ctgctcatca gaacgcttcg cgcttgattc ctggctttgc atgcgtttgt actgctcaac
     4141 taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc ctccagctga
     4201 agcttctata ttctcatcga acgaactcca gatcagcgcc gcaaataaga tgacatgcag
     4261 cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg cctttgacac
     4321 tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag
     4381 atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta tgtaacaagt
     4441 tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc agaaagaccg
     4501 ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct ggtggtaaac
     4561 gctccaggcg atctttctca accaccacgg tgtactgacc aataccagac acttcaacaa
     4621 tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct ggcagatcga
     4681 cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac agcaccagca
     4741 gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga cctcgtccac
     4801 gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca
     4861 gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg tttacgcgtt
     4921 ggttgagacg gttataagcc attaccgccg gaatagcggc gaacagaccg attgctgtag
     4981 caatcaacgc ttctgcgatg cctggcgcaa ccatttgcag cgttgcttgt tttaccgccc
     5041 cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca atatacgggc
     5101 tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca agttcacggt
     5161 tcatggaaat acgcatcgca cgcgacgccc cttctactac ggcttccggc gcatggctat
     5221 tggcacgatg caggcgcaca aattctttga acccgctgta aaagatttgt tccgaaccgg
     5281 tcagattatc ccgtttcccc tggctctctt gatagaggcg agagagttcg attccagacc
     5341 agaatttatc ctcaaacgct tcggcttcgc gcgccgctgc gttaagaata cgggtccgct
     5401 ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa
     5461 ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact gcttaaactc
     5521 cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac
     5581 cagaacctct gcttcattca gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac
     5641 caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat cgtcgagccg
     5701 cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc gttcagccat
     5761 cagcgcctgc tgactgaagt gatgatgacg cagcatctct gtgcgtgctc tttcataaaa
     5821 agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat agtagacgcg
     5881 aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa caaaagttag
     5941 agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa
     6001 gggagcgtaa aaatttatgg gcctggagcg gcccataatt ttttaaagag aattctgatt
     6061 taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa tgggcaaaaa
     6121 atcccttgcc aaagaacctt ctgcggacga aagcccacgc cgtgaatcac accagcacag
     6181 acggcccaca tcagcaataa accatgccag atttccagtt cactggtctt cgcggcgaaa
     6241 cgagatgggt cccaaaacat acatcctgct aacagaagcg ccatcacgaa ggaaagcgcc
     6301 cgcagggggc gcttgtccat taccgcatac aaggttgcga taatcttact catcagatgt
     6361 cttcttgacc ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg attaccccaa
     6421 acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt agctccttac
     6481 ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac cgaacatttt
     6541 ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg caacccaggt
     6601 catgacgtta agtgtcagct ggctggaagt tgcatcccac attgtcagac ttgcgttcat
     6661 catggtgctg gacggcatca caaacgggaa cattgcgata ccggcagtca ggatgatgca
     6721 ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac gtgcagtcag
     6781 gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa ttggcgtgtt
     6841 gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca gcgggttaga
     6901 ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt acatcaccca
     6961 tacgccagct agtgcgaagc agaccagtgt caccagtgcc gccacctgag ccgttgcacg
     7021 ggtacgcagg tgcagttcgc ccacggtacg catctgcaga taagtcgcgc cctgagtaat
     7081 gatcatccct acgctcacca cgcctgccag caggccgaac gggttaagca actggaagaa
     7141 gttaccggtg tagtacagac gcagatattc atcaacgttg aacggtacgc cctgcaacag
     7201 gttaccgaac gctacaccaa tcaccaacgg cggaacgaag ctaccaatga agatgcccca
     7261 gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa aaccgaccgg
     7321 acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg agaacgcagc
     7381 ggcatagacc atcggccagg cagcaaagag tgcgccgcct gcggtgatca gccaaacctg
     7441 gttaccgtcc cagtgtggag caatggagtt aatcataatt cgacgctcgg tgtcgttacg
     7501 accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccatcagtga ctgcaaaacc
     7561 aatcagcaga acgccaacca gcagccacca gataaaacgc aatacttcat aatcgatcat
     7621 ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct caaagtgata
     7681 gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta agaacaattc
     7741 tgccaccagg aacagggtat ataggccgca aatcagcacc attgagaaga tgagatcgcc
     7801 tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag cccacggttg
     7861 gcggccatat tcagccacga accagcccgc ttctacagca atccacggca gcggaatacc
     7921 gtacagcgcg gcgcgcagca gccatttttt ctcgccaatg cggttgcgga tgacactcca
     7981 gaaagagagc gcgatgattg ccagcagcag gaagccacac gccaccatga tacggaacgc
     8041 gaagtacagc ggcgctacac gcggaatgga gtctttggtt gcctgttgaa tctgcgcttc
     8101 agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac cgaggtcttt
     8161 cttcatgcta ttgaactggt cacgaaccgc ctggtcggta gaaccagaac gcagttgctc
     8221 gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca ccatcagttc
     8281 tttcaggccg ataactggag tatccacgga acgcgttgca atgatgccca gcgcgtaagg
     8341 gatctggatc gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga acagagtaaa
     8401 ggcagcaggc gcaggttgcg tttcccactc agcttcaata gcagccagtt tggttttctg
     8461 cacgtcgccc atttcgtaac cagattcatc acccagaaca ataacagaca gaacggcagc
     8521 cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt cacgaccttt
     8581 cagcatatac catgcgctga taccgaggat gaacatcgcg ccagtaacat aaccagacgc
     8641 tacagtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg agaagctcac
     8701 catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt gcatccagcc
     8761 gttcgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca gccaggtgac
     8821 acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac ctacaaaggt
     8881 ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga agatatcccc
     8941 tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg tcagaccggt
     9001 agccacaccc agagcgaagt tgataccaaa caacttgccc cagaacttgg tcatatcttt
     9061 ataaatctgt ttgccggaaa ggacgtagac cgtttccata atggccagca ggaacgccat
     9121 accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa actgtaagcg
     9181 cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag actccgagag
     9241 tgaaccccgt tagaaagggt cacacgcatg ccccaataca aaattatttg ctcgcctcta
     9301 tcgttgccat ttcatcaaaa gaggaaaaga caatgataaa aaagttacaa atatgttaat
     9361 gaat
//
LOCUS       DAPGRN010000154         9362 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPGRN010000154 DAPGRN010000000
VERSION     DAPGRN010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.156"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            138..500
                     /locus_tag="RHW33_004183"
     CDS             138..500
                     /locus_tag="RHW33_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647631.1"
                     /translation="MNEFEKIFNEMNLDRALLPILFRSNRSTVWKYLSGDSTAPASAM
                     SLIMLLQLIQKRNPDLLAEWLTLSDFTIPPEVYLDQPDYWKGWVYTQHKVNKNVLEYL
                     KKHYPDEDQKGMGKTTEE"
     gene            complement(781..1239)
                     /locus_tag="RHW33_004184"
     CDS             complement(781..1239)
                     /locus_tag="RHW33_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV1647632.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLIIRAVIA"
     gene            complement(1236..2153)
                     /locus_tag="RHW33_004185"
     CDS             complement(1236..2153)
                     /locus_tag="RHW33_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV1647633.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            2299..2976
                     /gene="fetA"
                     /locus_tag="RHW33_004186"
     CDS             2299..2976
                     /gene="fetA"
                     /locus_tag="RHW33_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV1647634.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            2963..3742
                     /gene="fetB"
                     /locus_tag="RHW33_004187"
     CDS             2963..3742
                     /gene="fetB"
                     /locus_tag="RHW33_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV1647635.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(3805..4659)
                     /gene="cnoX"
                     /locus_tag="RHW33_004188"
     CDS             complement(3805..4659)
                     /gene="cnoX"
                     /locus_tag="RHW33_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV1647636.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(4720..5529)
                     /gene="ybbO"
                     /locus_tag="RHW33_004189"
     CDS             complement(4720..5529)
                     /gene="ybbO"
                     /locus_tag="RHW33_004189"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV1647637.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(5519..6142)
                     /gene="tesA"
                     /locus_tag="RHW33_004190"
     CDS             complement(5519..6142)
                     /gene="tesA"
                     /locus_tag="RHW33_004190"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV1647638.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6113..6799
                     /locus_tag="RHW33_004191"
     CDS             6113..6799
                     /locus_tag="RHW33_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV1647639.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6796..9210
                     /locus_tag="RHW33_004192"
     CDS             6796..9210
                     /locus_tag="RHW33_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV1647640.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DNIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCCGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2129 a         2410 c         2571 g         2252 t
ORIGIN      
        1 gagtaaagac ttgttttgat aagcgaaata aattctgttt ctggactaaa cgtgaagcgg
       61 ttcctgatgt gtgctaaaat gcgtttcttt gataaatctc aatttttaag aggtttacgc
      121 caggcaggaa aatttaaatg aatgaatttg agaagatttt taatgaaatg aatctggacc
      181 gggcattgct accgatcctt tttcgttcga accggtcgac agtgtggaag tatttaagcg
      241 gcgatagtac ggctcctgcc tctgccatgt cgctgattat gctgttgcag ttgatccaga
      301 aacgaaaccc cgatctgtta gccgaatggt taacgctttc tgatttcacc attccgccag
      361 aagtttacct ggaccagcct gattactgga aagggtgggt gtatacccaa cacaaggtca
      421 ataaaaacgt cctggaatat ctaaaaaaac actatccgga tgaagaccaa aaaggtatgg
      481 gtaaaaccac agaagagtaa attgtcatta cccgcccaca tgccggatgc gcacagactg
      541 gcagtgttag taggcatgat aatacgcgtc agcgtcgcat caggcatcgg cgcataaccg
      601 ccggatgcga cgtaaacgcc ttatccggcc tacgattcgg gcgcagactt ccaggcatga
      661 taatacgcgc ccgcctcgca tcaggtgtcg gtgcacgact gccggatgcg gcgcaagcgc
      721 cttatctggc ctacgattcg ggcatatttt gtaggcatga taagacgcgt cagcgtcgca
      781 tcaggcgatg accgcacgga tgatcagcgt tatcccttct atcgcaatga cttcaacatg
      841 cgtacctgcg ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat
      901 atgaccgcgc ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg
      961 cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca
     1021 tttccaccac agccaggcgg cgagcagcgt caggatggca aacatcaccc cttgccactc
     1081 ccaacccagc ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca
     1141 caacaaataa ccatttccgc ccagcatctc ggctgccagc agcaacccgc cgagactgag
     1201 ccagaaaata tgtggatgaa cgaccattaa ctccatcatg gctgagtccg cttgttggcg
     1261 ctgtctttca ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat
     1321 ggcatcatca ctactttgct gttactggac gaaccgatct gctgtaacgc ttcggtgtat
     1381 ttctgcgcta cgaagtagtt caccgcctga atatcaccgg aggcgatggc ttcagacacc
     1441 attttggtgg cgcgggcttc tgcttcagcg gaacgttcac gcgcttcagc ctgtaaaaac
     1501 gccgactgac gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg
     1561 aggatttccg cctgacggat cccttccgct tcaaggatgt aagcgcgttt ggtacgttcc
     1621 gctttcatct gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt
     1681 tcaatacggg tgactttaat cccccacggg ttagtggctt catcgacaat acgcagcagg
     1741 cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccagcacg
     1801 gtacggatgt tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa
     1861 gccgcgcgcg gcgcgtcaat cacctgaata aagcacacgg cgtcgatggt aacgttggcg
     1921 ttatctttcg agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc
     1981 ttgcgaccaa tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg
     2041 gtatagcgac caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc
     2101 gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa
     2161 acctcctgtt gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct
     2221 gattactgaa agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat
     2281 cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga
     2341 tatctggcgg gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa
     2401 tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct
     2461 tcattgatca gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta
     2521 aagccagaaa tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc
     2581 gatacggtat acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca
     2641 gccatttttc tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat
     2701 atcgccgagc tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt
     2761 atgccgaagg ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat
     2821 aacgttaatg agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg
     2881 acacacgata aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc
     2941 ggagaaatgc aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact
     3001 ggcattaatg ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga
     3061 gaaagatatt ctctggagcg tcgggcgcgc gataattcag ctgattattg tcggctatgt
     3121 gctgaagtat attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat
     3181 ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat
     3241 ctcatcgttt attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc
     3301 agggtcgatt gaatttatcc cgatgcaggt gatccctatc gccgggatga tagccggtaa
     3361 cgccatggta gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca
     3421 gcaacagatt caggagaaac tgagtcttgg cgcgacgccg aagcaggctt cagcgatatt
     3481 gattcgcgac agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg
     3541 cttagtgagt ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa
     3601 ggcgattaaa tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac
     3661 cattattgcc tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt
     3721 gacgcaattg aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg
     3781 gttcatgtgc cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt
     3841 cgacgccagt gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt
     3901 ctgaccgtct gcggcggtga gatctttacg cagatgcccg aacagcaact ccagtgcctc
     3961 ttcattgcgc ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc
     4021 tggattctcc gccacctgct gttgcaactg ttgaatttcc ggcgtgtctg ccgcctgctt
     4081 cagcagttcg atttgcgcca ccagcccctg atagcgggtg tcctgatcct gcaacggaat
     4141 ggttttcagc accgcttctg catcttcaga acgattcagt gcaatcagcg tttctgccag
     4201 cagcaggccg atttccccgt tctgattcga caactgccag gcgtctttca gtaatggcag
     4261 ggcgtcggtg taattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc
     4321 ttcgcgcggc agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg
     4381 gaagccatct accggttgcc cgttctggaa cagatacacg gtcggaatcg cgcgcagacc
     4441 aaactgggcg gcaatcatct gctccgcgtc gcagtccagc ttcgccagaa taaattgccc
     4501 gttgtactgc gccgcgaggc tttccaggat cggggttaac tgcaaacagt gctggctacg
     4561 ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag
     4621 gttagattcg ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga
     4681 tattttcttt gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc
     4741 catcacgcgc cccggcagca ggcgcttaag caccattact gcccaggtca ccagcgttac
     4801 cggatagcgc atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc
     4861 ttccggtccc aacgtaaagc gagcagcgat gccgggattt tcgactggtt tatcactttg
     4921 cgtctggttg acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac
     4981 tttaattccg ctgtggcgta gctccatgcg cagcgcgtct gaccacgcct ccagcgcata
     5041 tttactggcc gcgtaagcgc cacgacccgg cgtggagatt aatcccatca ccgatgatgt
     5101 catcacaata cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg
     5161 gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc tgcgcacggc tgatggtgga
     5221 aagggggcca tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt
     5281 cagggcgatc acctcgtcgg ctgcgcgatc aacactttct ggtgaatcca gatcgatcaa
     5341 cacgccggta aatcccatgc tgttcatgcg ctcaacatca tccggtttcc ggcaacctgc
     5401 cagcacatga aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga
     5461 acatccggta attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt
     5521 atgagtcatg atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct
     5581 gggcgtcgcg gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct
     5641 cttccataaa aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg
     5701 cgctaaaggc ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca
     5761 atggttcagc gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt
     5821 gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccagcgcg
     5881 gctgatgctg tttcagcaga gccggaaggc gcgccagccc ttgttgcgag gtgtcgccgc
     5941 tgatgctggc attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc
     6001 aggccgcgct ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg
     6061 tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa caggaagggc aaatgccagc
     6121 ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact
     6181 ctccatcctc accggagttg agctggttgt caaacgtggc gagaccatcg cgctggtggg
     6241 cgagtcggga tcgggtaagt caaccttgct ggcgatcctc gccgggctgg atgacggcag
     6301 cagtggcgaa gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc
     6361 aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca tttatgctaa ttcctaccct
     6421 taacgcgctg gaaaacgtcg agcttccggc gctgctgcgc ggtgagagta gcgcggaaag
     6481 tcgtaacgga gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct
     6541 tccggcacag ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaacgg
     6601 tcgacctgat gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agaccggcga
     6661 taaaattgcc gacctgctgt tttccctcaa ccgtgagcat ggcaccacgt tgattatggt
     6721 gacccacgac ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca
     6781 gttgcaggag gaagcatgat tgcacgctgg ttctggcgcg aatggcgttc gccgtcgcta
     6841 ttaattgtct ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc
     6901 agcgatcgca tggagaaggg cttaagccaa caaagccggg agtttatggc gggcgatcgg
     6961 gcgttgcgca gttcgcgcga agtgccgcaa gcatggctgg aggaagcgca aaagcgcggc
     7021 ctgaaagtcg gcaagcagct gactttcgcc accatgacat ttgcaggcga cacaccgcaa
     7081 ctggcgaacg tcaaagcggt ggataatatc tacccgatgt atggcgatct gcaaaccaat
     7141 ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg
     7201 cttaacctga aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga
     7261 gaagtgattc aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctaatg
     7321 atgaatctgg ctgatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg
     7381 cgttataaat tcggcggcag cgagaaccag ctcgacagct atgagaaatg gttgttacct
     7441 cagcttaaac ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga
     7501 tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca
     7561 gtggcagcag tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg
     7621 atcctcaaaa cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg
     7681 ttgatggtgc tggcgctttc agccgttacc ggtggggcga taggcctgtt gttcgaaaac
     7741 gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac taccgcctgc cagtctctgg
     7801 ccgtggctgt gggcgcttgg caccatgaca gtcatctcgc tgctggtggg gctacgaccg
     7861 taccgcttgt tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac
     7921 gtctggccgc tgaagtttta tttgccgatt gtcagtgtgg tggttgtgct gctgctcgcc
     7981 ggattaatgg gcggcagtat gctgctctgg gcggtgctgg cgggcgcggt agtactggct
     8041 ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac ttcgccgcat gacgctgaaa
     8101 tcgctgcctc tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc
     8161 cagctttcgg cattttcgct ctcctttatg ctgctggcac tgctgctggt gttgcgtggc
     8221 gatctgctcg accgctggca gcagcagcta cctccagaaa gcccgaacta ctttttaatc
     8281 aacatcgcca cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcagatagtc
     8341 ccggaatcgt tttatccggt ggtgcgggcg agactgacgg cgattaacgg caagccgaca
     8401 gaaggcaatg aagatgaggc gctcaatcgc gaactcaatc ttacctggca aaatacgcgg
     8461 cccgatcata atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg
     8521 gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc
     8581 gatacccagg agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg
     8641 cggcctaatt tctattttat tttccctgaa ggggcattag acgggcaacc gcagagctgg
     8701 cttaccagtt tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc
     8761 ccgaccatta gcctgttaga cattggcgcg attttaaaac aggtcggcca ggtgctggag
     8821 caggtaagtc gggcgctgga ggtgatggta gtgctggtca ccgcctgcgg tatgttgctg
     8881 ttgctggcac aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc
     8941 acactcggtg cggggaaaaa actgctgcgc acgacattgt ggtgcgagtt cgccatgctt
     9001 gggtttgttt ctggcctggt ggccgcaatt ggtgcggaaa cggcgctggc agtgttgcag
     9061 gcgaaagtgt ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc
     9121 tgcggtgcgc tgctgctgtc gcttttcggc ggctggctgg gcgcgagact ggttaagggt
     9181 aaggcgctgt tcaggcagtt tgcggggtga tgagagtaaa taaggggcgt tctggttgaa
     9241 tggaacgcct tgtttaaatt aattctacaa tatgtttcat tgatgatgtt attgaattgg
     9301 tgatttcctg tctttctatt gctgatatta atctgaatcg gtggagttta ataattttaa
     9361 at
//
LOCUS       DAPGRN010000155         9233 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPGRN010000155 DAPGRN010000000
VERSION     DAPGRN010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.157"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(96..419)
                     /locus_tag="RHW33_004193"
     CDS             complement(96..419)
                     /locus_tag="RHW33_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV1647641.1"
                     /translation="MKRTLLIAVWAIGLMSDSAIALTLNEARSQGRVGETLNGYLVAL
                     QTDAETQALVKDINEARNHSYQQLAKQNNVSTKEIAKLAGQKLVARAKSGQYVQGVNG
                     KWLRK"
     gene            complement(427..612)
                     /locus_tag="RHW33_004194"
     CDS             complement(427..612)
                     /locus_tag="RHW33_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV1647642.1"
                     /translation="MKIVIAAFASSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEDLLKTRSDLF"
     gene            complement(609..3248)
                     /locus_tag="RHW33_004195"
     CDS             complement(609..3248)
                     /locus_tag="RHW33_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV1647643.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPMQLTG
                     EAKQADLILYARLPAQVSGSLSDPTLIFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKEGWLANAGPMTLRLDKDTADAVAKDNMTAGTAINWLRYMEIS
                     RSSTRINLDNLGVLTMQANISGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQV
                     WLEQNTQLPETGCPSGKECEEKQ"
     gene            3456..4445
                     /gene="ldhA"
                     /locus_tag="RHW33_004196"
     CDS             3456..4445
                     /gene="ldhA"
                     /locus_tag="RHW33_004196"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV1647644.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4556..4978
                     /gene="hslJ"
                     /locus_tag="RHW33_004197"
     CDS             4556..4978
                     /gene="hslJ"
                     /locus_tag="RHW33_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV1647645.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4975..5241)
                     /locus_tag="RHW33_004198"
     CDS             complement(4975..5241)
                     /locus_tag="RHW33_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV1647646.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5515..9039
                     /gene="nifJ"
                     /locus_tag="RHW33_004199"
     CDS             5515..9039
                     /gene="nifJ"
                     /locus_tag="RHW33_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV1647647.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRRDQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2312 a         2403 c         2261 g         2257 t
ORIGIN      
        1 tttttgtaga aatacccacg gtaattaagc atgctaacgt cagtttaaat actgtaaaag
       61 atcattaata gatacatttt gtagccaact ataatttatt tgcgcaacca tttaccatta
      121 actccctgaa catattgccc cgacttcgcg cgagcaacaa gcttttgtcc agccagtttg
      181 gctatctctt tagtggatac attattttgc tttgccagtt gttggtaact atggttacga
      241 gcttcattaa tatcttttac taatgcctgt gtttcggcat ccgtttgtag cgccaccagg
      301 taaccgttaa gcgtttcacc tacccgcccc tgacttctgg cttcgttcag ggttaaagca
      361 attgcactgt cgctcatcag gccaatggcc catacagcta tgagtaatgt tcttttcatc
      421 atcacctcaa aatagatcgc tacgggtttt gagcaggtct tcaacgtcct tatccgcttt
      481 aatgatgatt tcatgctcaa ttttaacgtt catattaatg gtgattggct ctttcggcgc
      541 agccacttca atgcgaggcg tgcaaccaac caacataaaa gaggacgcaa acgcggctat
      601 cactattttc attgtttttc ctcacactct tttcctgatg ggcagccagt ttccggtagc
      661 tgggtattct gctccagcca tacctggaga ttatcgccaa agcgtaaact gcgccataac
      721 gtaaaaatat tctcttcatg gtgataatta aggtttaccg taccgctttt accatcgacg
      781 cgactggtgc ccgaaatgtt ggcctgcatg gttaacacac cgagattatc taaattaatc
      841 cttgttgatg agcgactgat ttccatataa cgcaaccagt tgatggcagt tccagcggtc
      901 atattgtctt tggccaccgc atctgcagtg tctttatcca gacgcagtgt catcggcccc
      961 gcgtttgcca accagccctc tttgacaatc catttttcat tgttcagcca taacggcaat
     1021 gcaccactaa atgccccgga catggcaaat tgtttcggat tgacggcgct aaccagttcg
     1081 ctggatgaga gattattcag ccgcaacagc gccgggtcat gttgcggcat acgtaattgt
     1141 ttcatcagca cgttaccgcc taacacatcg acgctaacat cagtcaacag caatggttct
     1201 tcttcagtcc acggataacg cccttgcaaa tcagccgtaa tatttttcgc tgtcaccaga
     1261 ttaatcactt cggcaattcg caacgtaacg gggccgcgag tccccagatg ccaggctcca
     1321 tcggcaaaac ggaaaggcag gacaaaatcg acaccgttaa cctgattatc tggcatccag
     1381 gcactaccgc ctttcaacac gccgtgccct cccgcgcgga atccttgttc aggcgcagca
     1441 gaaaatgcaa cctgagcata cagttcacca tcgcgtaagt tcatcttcca gtcgggtggc
     1501 accagcggct ggaatacagt cagtgattgt ttaggccacc aggcgttacc gcgcagacga
     1561 ataccgtccc agcgaccatt tacccgaacc gggccaatct caccagcatg taaatcgcct
     1621 ttaaagagga aatacgtcgg atcgcgccca tcgacgctaa attttaaggt tgatggcggt
     1681 aacacactgc cgccagtgaa cagcgtttgc ccggcgtcca gtgacagcgc gccgctgaag
     1741 gagggatgct gtgcgtcacg tacccagacg acgggcttgt cgagaattaa tcgcggcttt
     1801 tctaccgtca tcgtaccgta ttgtaactgg tcgaaaccgg tggagagatc ggtcagcgta
     1861 atcgtgctgt catgccactc accttttcct gcgacatccc aggtggcatt catcggtgta
     1921 aaactccctt ttccccagta gcgccactgc cagcggccag cgtcagggag aaaatcattc
     1981 gccagcccat ccatatgcag cacgaaatcg cccagttcat tttcatgcgc ctgcaaaatg
     2041 gcctgcaaac gtccgtcaac accacgttgg gtgactttta cacccgctaa aggccagcgg
     2101 atttcatcga tgtccagcga atcgatgact cttccctttg aacgaagtaa cgcgcctggc
     2161 tcaaagatca gcgttgggtc agacagactt ccacttacct gcgcaggtaa acgggcatat
     2221 aaaatgaggt ccgcctgttt cgcttcaccg gtcagttgca taggcagctg gctgttatcc
     2281 atgcttaatt ttcctgggcc aaaatttagc accgcgttgc ctttacccgc ttgcccctgg
     2341 gtcagcacac tcagtcgtcc gctgaccaga gcgttctcga gccctgcctg ccagttgtcg
     2401 actttgacac ccagtcggcc actcaaagga aaacctgcat acggccagct ccagcgacca
     2461 tcgcttacgg tcaattgttg acgagtaatt tgccacggca aatcgagcaa cggatcgccg
     2521 ttatcccgtg ccagcacaat caattgcccg ctattttcct gccagtccag ctcggcatcc
     2581 accagtgacg gttcctgcgg caagttcaac gtcgcagtag catgaccact tacaggaagt
     2641 ccatccggca ctagcggcat agtaaattcc cccaccagtt taaccggcgg ctgattttca
     2701 aacgcgacga catccagttc gctgactgta agttgttgcc ctttcagctg gccttgaaat
     2761 ttaacttttt cgccctgata acgcagttgc tggatatcag aggttaatgc gagagagagt
     2821 tttccctgcc attcctgcca gggagaaaaa atcagtttat caatattgat ccaggtgtta
     2881 ggcagcatgg actgccattg cgcgagggtt tttggagcag ctggcgattg ctccgtctgc
     2941 ggcaatttcg ccagacaagc agaatcaagt tctaccgtgc cgacgttcaa taaccagcgg
     3001 ctgggatgtg aaaggctggc gttggtgata tgcgcaagct gacaatctcc caccagataa
     3061 cggagatcgg gaatgattaa acctttacgc gtaatgcgtg ggctttcatc taatgcaata
     3121 cgtgtcccga gcggtagcca gatgcccgcc agcgtgggaa cccacagccc gagcgtcatc
     3181 agcagcgtca acggcacaag aataatcagt aataacagcg cgagaacggc tttatattta
     3241 cccagcatgg gtagttaata tcctgattta gcgaaaaatt aagcattcaa tacgggtatt
     3301 gtggcatgtt taaccgttca gttgaaggtt gcgcctacac taagcatagt tgttgatgaa
     3361 tttttcaata tcgccatagc tttcaattaa atttgaaatt ttgtaaaata tttttagtag
     3421 cttaaatgtg attcaacatc actggagaaa gtcttatgaa actcgccgtt tatagcacaa
     3481 aacagtacga caagaagtac ctgcaacagg tgaacgagtc ctttggcttt gagctggaat
     3541 tttttgactt tctgctgacg gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat
     3601 gtattttcgt aaacgatgac ggcagccgcc cggtgctgga agagctgaaa aagcacggcg
     3661 ttaaatatat cgccctgcgc tgtgccggtt tcaacaacgt cgaccttgac gcggcaaaag
     3721 aactggggct gaaagtagtc cgtgttccag cctatgatcc agaggccgtt gctgaacacg
     3781 ccatcggtat gatgatgacg ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg
     3841 acgccaactt ctctctagaa ggtctgactg gctttactat gtatggcaaa acggcaggcg
     3901 ttatcggtac cggtaaaatc ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc
     3961 gtctgctggc gttcgatccg tatccaagtg cagcggcgct ggaactcggt gtggagtatg
     4021 tcgatctgcc aaccctgttc tctgaatcag acgttatctc tctgcactgc ccgctgacac
     4081 cggaaaacta ccatctgttg aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga
     4141 tcgtcaatac cagtcgcggt gcattgattg attctcaggc agcaattgaa gcgctgaaaa
     4201 atcagaaaat tggttcgttg ggtatggacg tgtatgagaa cgaacgcgat ctgttctttg
     4261 aagataaatc caacgacgta attcaggatg acgtattccg tcgcctgtct gcctgccaca
     4321 acgtgctgtt taccgggcac caggcattcc tgacagcaga agctctgacc agtatttctc
     4381 agactacgct gcaaaactta agcaatctgg aaaaaggcga aacctgcccg aacgaactgg
     4441 tttaatcttg ccgctcccct gcattccagg ggagctgatt cagataatcc ccaatgacct
     4501 ttcattctct attcttaaaa tagtcctgag tcagaaactg taattgagaa caacaatgaa
     4561 gaaagtagcc gcgtttgttg cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa
     4621 aattgctgtt acgccagaac agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg
     4681 taagcccgtg accagcgata aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat
     4741 ttccggcagc atgtgtaacc gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac
     4801 agccaaaggg ctggcaatga cccgtatgat gtgcgctaac ccgcagctta atgaactcga
     4861 taacaccatt agcgaaatgc tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt
     4921 aacgctggcg accgcgaaac agacattaac ttataagctg gcggatttaa tgaattaata
     4981 gctgccacag ctcccggcgg caagagactg ttcgctacag cgtttgccgt tgggtaatgc
     5041 acacatccca atcgccgtac catccagttg acgggcaaca gaaagcgaac cgccgatcat
     5101 tgcacaattt gcttctccac tactggacat cgacgctttt aaacctggcg ctacgtgcgc
     5161 cgctgtcgcc tgctgaacag gctcactact acacgccgac aacaataaag cggcacaccc
     5221 tacccaaaac gctgctcgca tctcttcttc ctctgatctt caagccaaac gacaccgcca
     5281 taaataatag gcagcacaga gggcgacgtc gagagctgtc ctgcgcgttg ccccgcattt
     5341 ttactttttt atggctattt ttttgccctc tgtttgatca aaacattcat tacgctgatg
     5401 tgggggacac aaaagcgaaa atgcagaaga aagccatttg ctaaaattga aagattacta
     5461 ctgggcgcgc agcaatttcg tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt
     5521 actattgacg gtaatggcgc ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc
     5581 atctacccta ttacccccag ttccacgatg gcagaacagg ctgatgcctg ggccggaaac
     5641 ggcttgaaga acgtttgggg agacacacca cgcgtggttg aaatgcagtc ggaagcgggt
     5701 gctatcgcta ccgtgcatgg cgctttgcag acgggtgccc tttcaacatc gtttacgtca
     5761 tcgcagggtt tgctgctgat gatcccgacg ctgtacaaac tggcaggcga actgacgcca
     5821 tttgtcctgc atgtagcggc acgtaccgtt gccacacatg cactctctat ttttggcgat
     5881 cattccgacg ttatggcggt gcgccagacg ggttgcgcga tgttgtgtgc agcaaacgtc
     5941 caggaagcgc aagactttgc tctcatttcg cacatcgcga cgctgaagag ccgcgtgcca
     6001 tttattcatt tctttgatgg tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg
     6061 gccgatgaca cgattcttga tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg
     6121 gcgctcaacc cggaacatcc ggtgatccgc ggtacgtccg ccaatcctga cacttatttc
     6181 cagtctcgcg aagccaccaa cccatggtac aacgcggtct atgaccatgt tgaacaggcg
     6241 atgaatgatt tctctgccgc gacaggtcgt cagtatcagc cgtttgaata ttacgggcat
     6301 ccgcaagcgg aacgggtgat tatcctgatg ggctctgcca ttggcacctg tgaagaagtg
     6361 gttgatgaat tgctaacccg tggcgaaaaa gttggcgtgc tgaaagttcg cctgtaccgt
     6421 cccttctccg ccaaacactt gctgcaagct ctgccgggat ccgtacgcag cgtggcggta
     6481 ctggacagaa ccaaagaacc cggtgcccag gcagaaccgc tctatctgga tgtaatgacc
     6541 gcactggcag aagcctttaa taatggcgag cgcgaaactc tgccccgtgt cattggtggg
     6601 cgctatggtc tttcatccaa agaatttggc ccggactgtg tactggcggt atttgccgag
     6661 ctcaacgcgg ctaaaccgaa agcgcgcttt acggttggta tttacgatga tgtgaccaat
     6721 ctgtcactgc cgttgccgga aaacaccctg ccaaactcgg cgaaactgga agccttattt
     6781 tatggccttg gtagtgatgg cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt
     6841 aattccacgc cgtggtacgc acagggctat tttgtttacg actccaaaaa ggcgggcggc
     6901 ctgacggttt ctcaccttcg agtgagcgaa cagccgattc gttccgctta tctcatttcc
     6961 caggctgatt ttgttggctg ccaccaattg cagtttatcg ataaatatca gatggctgag
     7021 cgtttaaaac ctggcggcat tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg
     7081 tcgcgcttgc cgcaagaagt tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg
     7141 attaacgcag cgaaaatcgc ccgcgaatgt ggcctggcgg cccgtattaa taccgtcatg
     7201 cagatggctt ttttccatct gacgcaaatt ctgcctggcg atagcgccct cgcagaattg
     7261 cagggtgcga ttgccaaaag ttacagtagc aaaggtcagg atctggtgga acgcaactgg
     7321 caggctctgg cgctggcgcg tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg
     7381 cacagcgcca atcgaccgcc agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta
     7441 accgctgcga tgctcgccgg gcttggtgat gccctccccg tttcggcgct gccgccagac
     7501 ggcacctggc cgatgggcac cactcgctgg gaaaaacgca acatcgccga agagatcccc
     7561 atctggaaag aggaactctg tacccaatgt aaccactgcg ttgccgcttg cccacactca
     7621 gctattcgcg caaaagtggt accacccgaa gcgatggaaa acgcccctgc cagcctgcat
     7681 tcgctggatg tgaaatcgcg tgatatgcgc gggcagaaat atgtcttgca ggtggcaccg
     7741 gaagattgca ccggttgtaa cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat
     7801 ccagagatta aagccatcaa tatgatgtct cgcctggaac atgtcgaaga agagaaaatc
     7861 aattacgatt tcttcctcaa cctgccagaa atagaccgta gcaaactgga acgtattgat
     7921 attcgtacat cgcagctgat tacaccgctg tttgaatatt ccggtgcttg ctccggttgt
     7981 ggcgagacgc cgtatattaa attgctgact cagctctatg gcgaccggat gttgatcgct
     8041 aacgccaccg gctgttcttc aatttatggc ggtaacctgc cctctacacc gtataccacc
     8101 gatgccaacg gtcgtgggcc ggcatgggcg aactctctat ttgaagataa tgccgaattt
     8161 ggccttggtt tccgcctgac ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat
     8221 caatttgccg ataaaatccc ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca
     8281 gaggttcgtc gtgatcaggt tgcagcttta cgccagcaac tcaacgatgt tgccgaagca
     8341 catgaactgc tgcgtgacgc agatgcactg gtagaaaaat caatctggct gattggtggc
     8401 gacggctggg cttacgatat cggctttggc ggtctggatc atgtattgag tttgacggaa
     8461 aacgtcaaca ttctggtgct ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg
     8521 aaagcgacac cgctgggtgc agtaactaaa tttggcgagc acggcaaacg taaagcgcgt
     8581 aaagatcttg gcgtcagtat gatgatgtac ggtcatgttt atgtggcgca gatttctctc
     8641 ggcgcgcagc tgaaccagac ggtgaaagcg attcaggaag cggaagcgta tccgggacca
     8701 tcgctgatca ttgcttatag cccgtgtgaa gagcatggtt acgatctggc actcagtcac
     8761 gaccagatgc gccaactcac agctaccggc ttctggccgc tatatcgctt cgatccgcgt
     8821 cgtgccgatg aaggcaaact gccgctggcg ctggattcac gcccgccgtc agaagcactg
     8881 gaagaaacgt tacttcacga gcaacgtttc cgtcgtctga attcgcagca gccagaagtg
     8941 gcagaacagt tatggaaaga tgctgcagct gatttgcaaa aacgctatga cttcctggca
     9001 caaatggccg gaaaagcgga aaaaagcaac accgattaaa tgctctggat aaggattatc
     9061 caattctaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     9121 agtggcaatg caatggccca acaaaaaatg aaatctgtga caataaaggc atataacccg
     9181 cgcagaataa cgtatagaaa attaaaagta taatttttat tttgtatgta tta
//
LOCUS       DAPGRN010000156         9229 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPGRN010000156 DAPGRN010000000
VERSION     DAPGRN010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.158"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..1290)
                     /locus_tag="RHW33_004200"
     CDS             complement(91..1290)
                     /locus_tag="RHW33_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV1647648.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITT
                     ESQWRSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDIS
                     LLRYMPA"
     gene            complement(1300..1488)
                     /locus_tag="RHW33_004201"
     CDS             complement(1300..1488)
                     /locus_tag="RHW33_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647649.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSI
                     LKRRGDTAALTLLQDTGV"
     gene            1605..2738
                     /locus_tag="RHW33_004202"
                     /pseudo
     CDS             1605..2738
                     /locus_tag="RHW33_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2808..3683
                     /locus_tag="RHW33_004203"
     CDS             2808..3683
                     /locus_tag="RHW33_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV1647650.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(3837..4652)
                     /locus_tag="RHW33_004204"
     CDS             complement(3837..4652)
                     /locus_tag="RHW33_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV1647651.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4988..5824)
                     /locus_tag="RHW33_004205"
     CDS             complement(4988..5824)
                     /locus_tag="RHW33_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV1647652.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5824..6627)
                     /locus_tag="RHW33_004206"
                     /note="aph(3'')-Ib"
     CDS             complement(5824..6627)
                     /locus_tag="RHW33_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV1647653.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            6913..8121
                     /locus_tag="RHW33_004207"
     CDS             6913..8121
                     /locus_tag="RHW33_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDV1647654.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            8149..>9229
                     /gene="gltS"
                     /locus_tag="RHW33_004208"
     CDS             8149..>9229
                     /gene="gltS"
                     /locus_tag="RHW33_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV1647655.1"
                     /translation="MFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFTFDA
                     SLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMMNES
                     PFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGGPVA
                     RHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYISAL
                     FKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALMSLK
                     FGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGATPTA
                     IANMQTVTKAFGPSHKAFLVVPMVGA"
BASE COUNT         2259 a         2477 c         2287 g         2206 t
ORIGIN      
        1 cggtggtttc cctctctgta tggtcgattt ttataccgta acaggctgtt ttttatactt
       61 tcaccgattc ccgggcacac ttagcctgtg tcaggccggc atatacctca gtagactgat
      121 atcccgcgcg tttttcttca gccctgatac attcagcccc gcaatggccc ggcggtatac
      181 catccggcag ccacacagca cgcattcgaa cgggtcacga ctcaggaact gtttcaccat
      241 ttgtgcatag cacactttcg ccactggttc cggtttatcc atccccagtg cacggtacac
      301 ctgcggcagc ttctctccac acacacggtt ggcaaggaac ccgaagtacc tcaccatctt
      361 aaaaaacttc tccgggatgt gctgtttcag cctcgcgacc agctcacgct gtgtcagcgt
      421 ttccgtcgcc gtttctcccg ttttgtggtc caggtaacgg aagctcaggc ttgcccctcc
      481 gttgtaatga gccagtcggg aagccgctat tggtggcttc ttcagataac gacccaggta
      541 gcgtgccgta ttccgccctc cggctgtctt cttcgacatg tacacatgcc agtattttcc
      601 gccggatttc agcaccaggc ttctccactg tgattccgtc gtgatatgtg acaacgactc
      661 cggcattgcc atcccctctg accacgcttt cagaagcagc tgccgcatat tccacatcca
      721 ccgtgaacgc atcgcgtctt tcaggaagct cagctttttc cactgaccat gcttattcag
      781 acctccacag gttacagaca catgtacatg cggatgccag ttgagacgac ggccatacgt
      841 gtggatggcg cagaagatac cgggttccag cccccgtttt cgggcggcat acagcagatt
      901 ctccaccgcc agacggcaca cgtcattcag cagccagcgg ttgctttcga acaccggcca
      961 cagcgtgtcc ggcagggtga agaccagatg tacccagtcg cagtcaggaa gacgattcag
     1021 ctgtgttgct gtccacaggt ctgtggcctt ctttccgcag gacgggcagg cacggctgcc
     1081 gcatgagttg gtcaggtact ttacgtgctg acagtccggg ttatcacacc cgaactcttt
     1141 tacacccagt atccgtgtgc cgcaggccag cattttggtg acggcttcaa cctcgatatc
     1201 gcgcagaccg cccgcatcca ggaaggacgt ccagcactgg ttggctgtga acagacgttt
     1261 cagagggcgg ggagtaaaac cggacaacat gaggatggat taaaccccgg tatcctgtag
     1321 cagggtaagg gctgctgtat cgccacggcg cttcaggatg gaggtcgtca ccatcgcagg
     1381 tggtgggtcc atgggtttga gcattttcat ccagtcccat tctcgttgtg agagcttctc
     1441 agcaccaaca gagagcagaa ctgtatcgat acttttgcgg gtcaacataa ggggatgagt
     1501 ccttaataaa acaggtcctc agagcataca tattctgcgg ggcgtgcggc aacaacagga
     1561 cgaaccgggg cggttttcaa tttgcagccg ccaggctgcc gtggttcacc catctgcgca
     1621 caggagcacc ctgcgctgac cggatcgggc taatgaacgt tatcttggcg gaagggatca
     1681 acctcggctt gcgcaaaatg gcggatgcga caaacaccca caccttctgg gaattgatcc
     1741 gcattggacg gtggcatgtc gagggcgaag cctatgaccg ggcgctggcc atggtggtcg
     1801 aggcacaggc agcgttaccc atggcccggt tctggggcat gggcacgtcg gcttcgagcg
     1861 acggacagtt cttcgtcgct acagagcaag gtgaggccat gaacctggtc aacgcgaaat
     1921 atggcaatac cccgggcctg aaagcctata gccacgtctc cgaccaatat gcgccgttcg
     1981 caacccaggt gattcctgca acggcaagcg aagcgcctta catcctcgat ggcctgctga
     2041 tgaacgatgc tggacgccat atccgcgagc agttcaccga cacgggcggc ttcaccgatc
     2101 acgtctttgc cgcatgtgcc attctcggct accggttcgc tccgcgcatc cgcgacctgc
     2161 catccaaacg gctctacgcg ttcaatccgt cggccgcccc ggcgcacctg cgagcgttga
     2221 tcggcggaaa ggtcaaccaa gccatgatcg agcgcaattg gcccgacatc ctgcgcatcg
     2281 ccgccaccat tgctgccggg accgtcgcgc caagccagat tctgcggaaa ctcgcctcct
     2341 atccgcggca gaacgagctc gcgacagccc tgcgggaagt cggtcgcgtc gagcgcaccc
     2401 tgttcatgat cgactggatt ctggatgccg aactccaacg gcgtgcccag atcgggctca
     2461 acaaaggcga agctcatcat gcgctgaagc gggcaatcag cttccaccgc cgcggtgaaa
     2521 tccgcgaccg ttccgccgaa ggccagcatt accgcatcgc cggcatgaat ctgctcgccg
     2581 ccatcatcat cttctggaac accatgaagc tcggcgaggt cgttgcaaac cagaaacgcg
     2641 atggaaagct gctatcgccc gatctcttgg cccatgtttc gccgctcgga tgggaacaca
     2701 tcaatctcac cggagaatat cgctggccaa agccttagcg taggattccg ccccctcccg
     2761 caaacgaccc cgtgatgacc ctgcaggcat cccctgagac ccggttaatg cgcgccgagg
     2821 tgcagcggca ttatccgcac atctggcagg agctggaggg gctggcgcag ggactggagg
     2881 ccactgttga tcaggtcttt gcctggaact gccgtggcga tctggtgcgc tcaacgtcgg
     2941 atggctgtac gacggtggcc ggggtaaacg ccgcaggcga gcggattatc gcgcataatg
     3001 aagacggttt tcctcagtta cgggatgaat gcttgctggc taccattact ccgagtcgcg
     3061 gtctgagctt tacgagtttt gcctatccgg ggtcgatttg cggccacacc tttgcggtaa
     3121 atgaaaaagg tatcgttaac acggtgaata acatccgtgc agtgtatcgt ccgcagggat
     3181 taccgcgtca gatcctggct cgcgcctcgc tgaatgcgaa caccctcgac gaggccatca
     3241 ctatacttac cggccagccg cgatccgggg cgtttcacca tacgctcggg cagctgggcg
     3301 atgcgcggct gttcagcgtt gaggccaccg gtcaaggctg ttccgttcta cccctgacga
     3361 cggtaaccgg gcatgccaac catctggtgc atgcggcact ggctggcgtt gagcagatcg
     3421 ttaccgacag ttcagcgtcg cggcagttac gcctcgtgca gtggcgggaa acgcaacctc
     3481 cgtttgacgc cgcggcggca aaaaccattc tctcagatac ccatgacgca gaactgccaa
     3541 tttaccggct ggctgccgac gatccggatg aggagaacac cctcgccacg gcagttttca
     3601 ccctcgatgc caaccacgtc aggtggcaaa ttttcgacat taaccgcgac gatgctaaat
     3661 ttcatggaga agtgcgtggg tgaaatagcc ggtcgtcagt cataaagggc aggggagtac
     3721 cgttggcccg gcgttcgata gtaccgtccg gatactgcca gatatcggcg tccagggaga
     3781 tgtcctgaag cgcggtgtcc ggaatttcag gtttgtgtct ctacaaagac taactatcag
     3841 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     3901 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaatt caccgaggca gttccatagg
     3961 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     4021 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     4081 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     4141 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     4201 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tactaacagg aatcgaatgc
     4261 aaccggcgca ggaacgctgc cagcgcatca acaatatttt cacctgaatc aggatattct
     4321 tctaatacct ggaatgctgt tttcccgggg agcgcagtgg tgagtaacca tgcatcatca
     4381 ggagtacgga taaaatgctt gatggtcgga agaggcataa atgccgtcag ccagtttagt
     4441 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     4501 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     4561 tcgcgagccc atttataccc atataaatca gcatccaggt tggaatttaa tcgcggcctc
     4621 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     4681 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     4741 ttttgagaca caacgtggat ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     4801 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc
     4861 aactggccca cctacaataa aggggtcgtt tgcgggagag ggcgaaatcc tacgctaagg
     4921 ctttggccaa cgatattctc cggtaagatt gatgtgttcc caggggatag gagaagtcgc
     4981 ttgatatcta gtatgacgtc tgtcgcacct gcttgatcgc ggccgcgata gctagatcgc
     5041 gttgctcctc ttctccatcc gcgttccaag ctgcggaaag gcacccataa gcgtacgcct
     5101 ggtcgagcag gcgacgcgga tcgacgtcca gcgcacgaga gaatgcgtcc gccatctgtg
     5161 caatgcgtct aggatcgaga caaaggtcgt ctctgtcagc cggatcgtag aacatattgg
     5221 cggcgccaaa gcccacttca ccgaccagac cgacgggatc tatcaccagc cagccgcgac
     5281 tggagaacat gatgttttca tgatgcagat cgccatgtag cccacgcagt tccgaggcat
     5341 tgctcatcat ttgatcggct ataatcgccg cgtggacgta gtcagtttga caacctgcgt
     5401 tttgatcatc gcgcgcccgc tgaaacaaag ctgcaaagcg atcccggatc gggagaaggg
     5461 cagaaggcag gggttcctca gatgcggcat acagcttcgc cattagttcc gctgcaattt
     5521 cggtcgcctg gtagtcgccg tgctcggcaa cgatgtgaga gagcattcgc tccccggcat
     5581 attcgagcaa catcagattg ttctcacgac cgagcaaccg gactgctccc ctcccattgc
     5641 gccataccag atagtcggcc ccgcgcagtt catcagcaat gtcttctata ggtttcaatc
     5701 ccttgacgat tgcaggagtc ccgtctggca atgaaacttt ccaaacgagg ctggaaaagg
     5761 tgtccgcaat gagaacaggt tgcgaaacgt gccaatgagc aggaaaaaca ggcggcatga
     5821 acatcaaccc caagtcagag ggtccaatcg cagatagaag gcaaggcgtt cgcggtcggg
     5881 ggcttcgatc cccaatacat tgaataggac agcgaaggcg cgctctgctt catctggcgc
     5941 tgcccagttc tcttcggcgt tagcaatcat gagtgccaaa tcggcatagc gatctgctgt
     6001 tccgagccgc ccaaggtcga tcagacccgt gcattgaaga gttttagggt ccaccatgaa
     6061 gttcggcatg cagggatcac catggcaaac aaccatatcg gtgcgctctt ggtcgagccg
     6121 caccggtagc tctcgttcga cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc
     6181 gtccggtaag aagtcgggat tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa
     6241 cattcgcgac agcctgcgct caaacggaca ttgatcaacc gataggctgt gaacagcgcc
     6301 aagttgctgc cccattgacg gccacgcttt gagcaaatcc gctccagaca gatcagccgc
     6361 cggtactccc ggaattgccg ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt
     6421 gatcacctcg gggcaagcca cacctcgacc tttgagccaa atgaggcggt cacgctctcc
     6481 agcgagctca ccgcggcggg aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg
     6541 tcgaaaaaca aaatcaccag attctccgcc tctgacaggc aaccagtcag aatgcgattc
     6601 accaaaaaaa atattagttc gattcaatgg aggttccttc agttttctga tgaagcgcgg
     6661 aggtggctca acctgcgaaa agaaacgagt tgctacgtaa gtccgagaac atgctttcca
     6721 tggtcttgca ccggttgtgt tccggtctat ctctcatttt aagcggcttt tttctcaaat
     6781 gccaccggcg atttccagcc gagtgctgat gaatccccta atgattttta tcaaaatcat
     6841 taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa
     6901 tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca attctgcccc
     6961 gaattacact taaaacgact caacagctta acgttggctt gccacgcctt acttgactgt
     7021 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat
     7081 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct
     7141 gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat tgtacttgtt
     7201 gactggtctg atatccgtga gcaaaaacgg cttatggtat tgagagcttc agtcgcacta
     7261 cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca atgttcaaag
     7321 aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa caccacaccg
     7381 ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga gaagctgggt
     7441 tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg agcggaaaac
     7501 tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac tttaggctat
     7561 aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa atctcgctct
     7621 aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc acctaaaatc
     7681 tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt tgaaattcga
     7741 acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga aaccttccga
     7801 gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag cagctcagag
     7861 cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg gcttgcgggc
     7921 gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt cagaaatcga
     7981 aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg ctacacaata
     8041 acaagggaag actcactcgt ggctgcaacc ctgcttactc aaaatctatt cacacatggt
     8101 tacgttttgg ggaaattatg aggggatctc tcagcgctac tcataggaat gtttgtcgta
     8161 aaatttaccc cgttcctaca aaaaaaccac ataccagaag ccgtcgttgg tggctttatt
     8221 gttgcaattg ttctgttaat tattgataaa acatcaggtt attcgtttac ttttgatgct
     8281 tcattgcaaa gtttattaat gctcacattc ttttcctcta tcgggctaag ttctgacttt
     8341 tctcgactga ttaaaggagg aaagccgtta gttctattaa ctattgcagt aacgatccta
     8401 atcgccattc aaaatactgt cggcatgagt atggctgtca tgatgaatga aagtccattt
     8461 attggcttaa ttgcaggttc aattactcta acaggtggtc atggtaatgc cggagcatgg
     8521 ggccctattc tcgctgataa atatggtgta acaggcgccg ttgaattagc gatggcttgt
     8581 gcaacacttg gattagtgtt gggtggttta gttggcggcc ctgttgcccg tcatcttttg
     8641 aaaaaggtct ctattcctaa aacaaccgag caagagcgcg acactatcgt tgaagctttt
     8701 gagcaaccaa gcgtcaaaag aaaaatcaat gcaaataacg ttattgaaac catttcaatg
     8761 ctgattatct gtattgttgt tggtggctat atcagtgcat tgtttaaaga tacttttctg
     8821 caactgccta cttttgtctg gtgtttattt gtcggtatta ttatccgtaa tacactgact
     8881 catgtattta aacacgaagt gtttgagccg accgtcgatg tattaggtag cgttgcttta
     8941 tcgcttttct tggcaatggc gttaatgtca ttaaaatttg gtcaattggc aagcatggca
     9001 gggccagtat taattatcat tgctgtacaa actgttgtca tggtgctatt tgcctgcttt
     9061 gtcaccttca aaatgatggg caaagattat gatgctgtcg tgatcagcgc gggtcactgt
     9121 ggctttggta tgggagcaac accaacagca attgcgaata tgcaaacagt cacaaaagca
     9181 tttggaccat cacataaagc tttccttgtc gttcctatgg tcggtgcct
//
LOCUS       DAPGRN010000157         8994 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPGRN010000157 DAPGRN010000000
VERSION     DAPGRN010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.159"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1056
                     /locus_tag="RHW33_004209"
     CDS             1..1056
                     /locus_tag="RHW33_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV1647656.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESSLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGRTFHYYSLAVMISAKQINNLISQDEFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNRLR"
     gene            complement(1158..2906)
                     /locus_tag="RHW33_004210"
     CDS             complement(1158..2906)
                     /locus_tag="RHW33_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV1647657.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLSRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQQLSTVCDNQHRWPLMQGDYLSSLILAIYALRNQLTDTWPQYPGNEIKQIV
                     EQSGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNHYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAETI
                     VGLTGYEGFMALLRQG"
     gene            complement(2906..3976)
                     /locus_tag="RHW33_004211"
     CDS             complement(2906..3976)
                     /locus_tag="RHW33_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV1647658.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GYKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGAKSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     TKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYLPNQKLVAALKNCAAH
                     NGLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3966..5417)
                     /locus_tag="RHW33_004212"
     CDS             complement(3966..5417)
                     /locus_tag="RHW33_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV1647659.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5428..5874)
                     /locus_tag="RHW33_004213"
     CDS             complement(5428..5874)
                     /locus_tag="RHW33_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV1647660.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            6352..7746
                     /locus_tag="RHW33_004214"
     CDS             6352..7746
                     /locus_tag="RHW33_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV1647661.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAESQVKQLQTQQAAEIREIKAAQGNTPVNGQTTAESAKKNATSPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPDNGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYTIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKKHGDDT
                     DDRYGARVRFKYFF"
     gene            complement(7787..8101)
                     /gene="rhaM"
                     /locus_tag="RHW33_004215"
     CDS             complement(7787..8101)
                     /gene="rhaM"
                     /locus_tag="RHW33_004215"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV1647662.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTEICQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            complement(8111..8935)
                     /gene="rhaD"
                     /locus_tag="RHW33_004216"
     CDS             complement(8111..8935)
                     /gene="rhaD"
                     /locus_tag="RHW33_004216"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV1647663.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNSKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
BASE COUNT         2321 a         2175 c         2305 g         2193 t
ORIGIN      
        1 atgaaaagga atttattatc ttccgctatt atagtcgcca tcatggccct cggtctgacg
       61 ggttgtgatg ataaaaaagc cgaaacagaa acgctcccgc ctgccaatag tcaacctgcc
      121 gcaccagctc ctgaagcgaa acctactgaa gctcccgttg caaaagcaga agctaaacct
      181 gaaacacctg cgcaaccggt ggtcgatgaa caagcggttt tcgacgaaaa aatggatgtc
      241 tatatcaagt gctacaacaa gttacagatc ccggtacagc gcagtctggc gcgttatgct
      301 gactggctga aagattttaa acaggggcct accggtgaag agcgtactgt ttatggcatc
      361 tacggcatta gtgaatccag cctcgctgag tgtgaaaaag gcgtaaaaag tgctgtggcg
      421 ttaacgcctg cgctgcaacc aattgatggc gtggcggtga gttatatcga tgctgccgtg
      481 gcgttgggta ataccattaa cgaaatggat aaatattaca cccaggaaaa ttataaagac
      541 gatgcgtttg cgaaagggaa aacgctgcac cagacattct taaaaaatct ggaagccttt
      601 gaacctgtag cagaatctta tcatgcggcg attcaggaaa ttaatgataa gcgccagctt
      661 gcggagctga aaaatattga agaaagagaa gggagaacat tccactacta ctctctggca
      721 gtcatgattt cagcgaaaca aattaataac ctgatatcgc aagatgagtt tgatgcagaa
      781 gcggcaatga agaaagtgtc tgaactggaa acgctggtgg cgcaggccaa agaagcggat
      841 aaaggcggca tgaatttctc gtttattaat tcggcaggcc agtatcaact cgaggctaaa
      901 aaatacgttc gccgcatcag agataaagtc ccgtactctg actgggataa agagcaactt
      961 caggacgcaa actcaagctg gatggtcgaa gactctttcc cgagagcatt acgcgagtac
     1021 aacgaaatgg tcgatgatta taatcgcctg cgttaaacgt ttttgttttt gctgagaaat
     1081 acaaggtcgg atgcggcgta aacgccttat ccgacctaaa cgtcgttaag ccgtgttagc
     1141 ggtgcatccg acatcattca cccctggcga agtaacgcca taaacccttc ataccccgtt
     1201 agtccgacaa tagtctccgc cggatgctga cacaatacgc ttgccagata gctaaatatt
     1261 tttagctcat gacgcgcatc ggcggcggcg caggcgatca agacatggtt aatgacttcg
     1321 ttattcacct caactggttg ggcgagggta ataaaaaatc cacgaaaacg tcgctcctgt
     1381 tcgctccagc aatgtgggat ggcgaggcga ttaacaatca ggttttcacc ttcgccttcg
     1441 cgggcgatga tccgttgcgc ttcatcggcg gtaatatggt gttgtgcgac taattgcgca
     1501 caaatttgcc gggtaatatg ttgccagctt tcaccgcgta cattcgaata atgaaaactt
     1561 ccgggggcag agaaaaaacg ctccggttgt tggcgaataa atgccgtcgc cagaaaatgt
     1621 tttatttgtt cgataccggc agccgtaatg atatttttta cggtgatgta atgatttacc
     1681 gcgtcatcca gtaaataatg gctgttgtta atgatcaata acggctcaat ctcttcgcga
     1741 atggcaacaa gctcgcttaa gctacgggca ataatcaccc gacaatgtaa aacatcgcgc
     1801 tcaatggcga gctgattaat agtggcaatc gcgttctggt ccgagagcaa aatgatcggc
     1861 tggcgttcgt tttgatgtcg ttccagcgca caggcaaaat acaaaccgat cagatcgctg
     1921 tcgaaaaggg gaatatcgag ctgttcacgc agtagcgtaa taaagtgcag actcatatca
     1981 aaggccgcag gccaggcggc tttcaggtta ttcatgctgc tttggcgatg ctcagcaatc
     2041 cacactggtg cggcaaggca acgtaataaa tgcccggtaa tattctcaac caactgcgta
     2101 tcaataatat tcaatgacgc gatgcctggc accctttgca gcaaccccag cacatgatcg
     2161 gcagaaatta cctgcgcttg ctgatgctgt ttctctatca aacctgtcag ggttcttaca
     2221 gcgttatcac caagaaacag gccgctctgt tcaacgattt gttttatctc gttaccggga
     2281 tattgcggcc atgtgtcagt aagctgatta cgcagggcgt aaatcgccag aatcaggctg
     2341 gagagataat caccctgcat cagcggccag cggtgttggt tgtcgcagac cgtggataat
     2401 tgctgaaggt tgtctcgtgt tacgcccgcc agcggtatta aaaacggatc tttgcgtagc
     2461 aagttcgcca gcaagataac gcgtttctct tctgtttcat caatgaaatg gcccaaaccg
     2521 gggcgactgg ccaggcaaca agtgcgttca taacgctgtt ttaaccggga cagacgctct
     2581 gctacccacg tttctggcaa attaagcgcc gaggcgagtt gcgcacgggg agtgaaagta
     2641 ttcagtaata acagcgccag cagccgatcg tcgttatcgt gcttttgcag caactgaaaa
     2701 aaactgcggc gctcgaagat ttccagctga tagcccgcac tgccactggc gaaaatgcgg
     2761 gctttgccgt taagggtgaa gttgagatag tcaatatcac gcaggatggt cctgcctgaa
     2821 acgccagtct gttgcgccag ctcgccaggc gtgcgcggct gttgctccag cagatcgaca
     2881 atctttaact ggcgttcgtt tagcattaat ccacctgacg gaactgacta aacgcgttga
     2941 ctttctccgc agttaaggtc gtcagaaaat cccgtatcag cgtgagcaga gcatcataat
     3001 cggcttttga aatcataccg ctgttagcgt gcagataacg agttggcaga cacagcgcga
     3061 caaccggacg cccgccgccc atcacgttgt agcgcccgcc atccgtcgct ccggttttca
     3121 tggtggaaaa ttgcagcggt aaaccgttat gtgcggcaca gttttttaac gccgccacca
     3181 gtttctggtt gggtaagtag cgcttgtcaa acagcatcag ccccggcccg ttgcccagtt
     3241 tcagcgggta tttaatgtta tcaatgcccg gaacatcacc tgcgacggcg gtatccagca
     3301 cgatcaccac atccggttta gtgtgttcag ccgaggtttg tgccccgcgt agcccaactt
     3361 cttcttccac actgccaacg ccatacagcg taatttctgg gttatttact gtctgtagta
     3421 gctccgccat catcgcgcag ccgatgcggt tatccagcgc cttgccgact actttatctt
     3481 cgccccagca ggcaaaattg gcttccgggc taataaaatc gccaatttcc acgccgcgct
     3541 tttccgcttc ttcgcgactt ttcgcgccaa tatcaatgaa catctcatca aatgacagcg
     3601 gttgttgctt ttgcttttcc gttaacgcat gaggcgcgac ggaaccaatc acaccaggga
     3661 ttttatatcc cttgtgtgtg cgtatggtta cccggtggtt gagcatcgac tgattccacc
     3721 agccgccaat ggtggtaaag cgcagaaaac cgctctcgtc gatgtgggtg accataaagc
     3781 cgacttcatc catatgcccg acaacggcaa cttttggacc tttatttccc ttacgggcaa
     3841 caaagctgcc cagaccatca aaggtgatct cattaacgca aggttccagc gtgtttatca
     3901 gaatgtcgcg aacttcctgt tcatcgccgc tgacggcgct ggcttcgcac aactgttgca
     3961 gtaactcaat gttcatggac aagctcctgt tgcgcggctt tcgctttgcg gcgtaaccac
     4021 aatcctttca ggacgataat gaatgaaatg ttcagcgcga ggccaatcgc cagcaccaga
     4081 taaaacgaac ccaccggcga catcaggccg atcagcgggt caaagatacc taaaccaggt
     4141 gccagacgtt tgattccgaa agcgatcacc agcacgccgg taatgccgcc cgccagcgta
     4201 ttggcggtaa tcatcggcaa cggagctgcc agagcgtaag gaatagccgg ttcggttgcg
     4261 actgtggcac cgaccacaat ggcactactg gcagcctctt tttcctgtcg ggtaaacagt
     4321 ttgggcgcaa tgaacgtcgc aagacccgcc gcgacgggcg gcatcagcgc gacaacgccg
     4381 acgatggcgt accagtcata aatgtgtttt tccagcagtg agaagcagaa gaaccaggcg
     4441 gttttattga tggggccgcc catatcgaac gccagcatcg caccgaccag aaacgctgcc
     4501 gcaaacttca ttgatggagg aatggtattc aggaagtgga gcagcccgcc catgaggtcg
     4561 gacatcaccg gtccgataac gtagtaagtg agcacgccga aaaccagcag agtaacgaac
     4621 gggatcagca ttgaaccgag cagcggttgt aatgctttgc caagacggac tttacggaac
     4681 cagaagacga aatagccaat cgccagcccc agcaccaccg cgccgaggaa tcccgcaccc
     4741 gactgggtgc cgagcagcgc tttgtcgttc gccagatagc aaaccagaaa cgccggagcg
     4801 aaagccggtt tatcggcaat cgacgaggca atataagcgc ccatgatggg gatcataaag
     4861 gtgaagccaa ggtaaccgat ggattcaact acccaggtaa acgacggcgc gcctttcgac
     4921 atatcggtat acggcaaacc gaactgcacc agcatgttgg caagcgccac caggatacca
     4981 ccgccgatga caaacggcag cgccgcagag acgcccgcca tcaggtggct catcacgctg
     5041 ccgctttgta cttcctgttt gcctagcttc acgccgctat ctgcggcaaa aagctgcgag
     5101 tttgttggta attcgctgaa aatctgatcg atatttttca gcgcctggga gatggcaatc
     5161 tcataaactt ttttcccggc aaaccgtgcg cgatcgtcac cgctcaggcc acgcccggtg
     5221 gcgagaatca cgtaatcggc agcggcaatc tcttcgctgg ataagcggtt ttcaacgccg
     5281 ctggaccctt gagtttccac ttttatggta tggccgagag aacgggcttt ctgttccagg
     5341 gcttccgcca ccatgtaggt gtgagcgatc ccggcggggc agttggtgat cgcgacaata
     5401 cgtaaggatg actccatcgc cacttcctta tgagctgagg gtttgattta acaaggcaag
     5461 cacctggtcg gcgtcgccct gttgcagttg gtgaataaaa tcctggtgaa taattttgcg
     5521 acacagtgtg ccgatgattt tgacctgatc ctcttcgcca ctttgcggca cgccgaggca
     5581 gatccagcaa ttgacgtctt cgccgtcgct ggctttccag tcaatagcct gcgctttgcg
     5641 ggcgaataac acgaacggtt gtttaacgca ggcgctttta ccgtgcggca cggcgacacc
     5701 agaaccaaaa ccggtggagt gcattttttc gcgcagcagc agggtttgca ggaactggtg
     5761 tgagtcggtg ataaaaccgt tttgtaacgc cattgtcgcc aactgcttca gaacggaata
     5821 agcgccattg ccctgaatat tcaggtcaat acagcttgca gtaagagctg ccattaatcg
     5881 gactccatga aaaaatattt cagtttattg taatagcggg gcgggtttgt gatgagcgcg
     5941 gatatgtcgt gaagtcgtga caatataaag cagagtgaag gataaatttt gcggcgtaag
     6001 gcacatatta gtaggtaaaa taaatcttgt gaataaagag ttattattta atgattttgt
     6061 gaatgtttaa atatctcaga taatttgtct aaacgttatt tatatttgtg gcttatgagt
     6121 cagcttgaat catatctaat attttttacg atatttagtt aaaaacggca ctattgttat
     6181 attcctcacg ttccaggaaa tcagcggatt tactcatatt atgcggcggg atactatcca
     6241 tcggcctgct gaagttggat taaaaattcg aaagtgcaca ctttatattt ctctaaatac
     6301 gtattccttt tggaaagcgt agggcctcct ttatttcagg gaacgtaaat aatgaaaaaa
     6361 tcgacattat ctttagccat cggtttatta ttggcatgta gtaccggtat ggcaaaaaca
     6421 cagcatttaa cgctggaaca acgcatggca ttgctggaag aacgcctgga agcggcagaa
     6481 atgcgggcgg caaaagcaga gagccaggtt aaacagctgc agacacaaca agccgctgag
     6541 atccgcgaaa ttaaggctgc ccagggcaat acgccggtaa atggacagac aacagcggaa
     6601 tctgcaaaga aaaatgccac ctcacctaat cttttgcttt caggttatgg cgatttaaaa
     6661 atctacggcg atgtagaatt taatatggat gcggaaagta atcatggcct gctggcaatg
     6721 accaacgctg atgtgaatag cgatcccact aatgagcagt ggaatctcaa tggtcgtatt
     6781 ctgttaggtt ttgatggtat gcgaaaactg gataatggct atttcgccgg gttctccgca
     6841 caaccgctgg gggatatgca cggttcagta aatatcgatg atgcggtatt cttctttggc
     6901 aaagaaaacg actggaaggt caaagtaggc cgttttgaag cctacgatat gttcccgctg
     6961 aatcaggata cctttgttga acattccggt aatactgcga acgatcttta tgacgatggc
     7021 agcggttata tctatatgat gaaagagggc cgcgggcgct ctaacgctgg cggtaatttc
     7081 ctcgtcagca aacaactcga taactggtat tttgaattaa acacgttact tgaagacgga
     7141 acatctttat ataatgacgg taattatcat ggacgcgata tggagcagca gaaaaatgtt
     7201 gcttatctgc gcccagtaat tgcctggtcg ccgacggaag aattcaccgt ttccgcagcg
     7261 atggaagcga acgtggtaaa taatgcttat ggttataccg atagcaaggg taattttgtc
     7321 gatcagtccg atcgtaccgg ctatggtatg agcatgacct ggaacggcct gaaaacggat
     7381 ccggacaatg gcattgtggt taatcttaat accgcctatt tagatgctaa taatgagaaa
     7441 gatttcactg ccgggattaa cgcgctgtgg aaacgtttcg agctaggtta tatctacgcg
     7501 cacaataaga ttgatgaatt cagcggtgta gtttgtgata acgactgctg gattgatgat
     7561 gaagggacgt acaccattca caccattcat gcgtcttatc agttcgctaa tgtgatggat
     7621 atggagaact ttaatattta cctcgggacg tattactcca ttctggatag cgacggcgat
     7681 aaaaaacacg gtgatgatac tgatgaccgt tacggcgcac gcgttcgctt taaatacttc
     7741 ttctgatgaa ggcgcggtgc tggatatttt ccggcaccgc aactcattaa ggcaggtaaa
     7801 acacttcttg cagctcgcta ctcaccgggc tgttatccgg gttagcgggc ataacgtcgg
     7861 tcatatattt ccaccaacgt tggcagattt cagtgctggc taccgcattc caccgttctt
     7921 cagattcaat ctctaccatg gcaaacagca gattacgcgc tttgtcgagg taaatggcgt
     7981 agttatgcgc accgtgagat ttcaacactg cttccagttc cggccagatg gggttatgcc
     8041 gacgctgata ctcttcgtgg gcgtcggggt ttacctgcat gacaaaggct ttgcggatca
     8101 tgtttgctcc ttacagcgcc agcgcactgg cgagtggtgt aacgccgaaa cgctggccga
     8161 gcgctatcaa ctcttcacgg ctgatagtct gtttcatgcc gcccatcgaa taaaccttca
     8221 ctaatacttg tgctgatttt tctgcggtgt cgattaaacc gaaggtttca tccagcgtcg
     8281 gtccggaacc gaacacaccg tggaagggcc acaacaccag cgaatgtttt tgcatctctt
     8341 gggcagttgc ctggccgatt tcgtccgtgc cgggtaccat ccacggcaga atgccaacgc
     8401 catccgggaa taccaccaga cactcggtgc tgccttccca cagttggcga gtgaagaccg
     8461 cggtgtcgtt ttcaagtaca taggtgaggg cgatcaggtt ggtggcgtgg cagtgcatga
     8521 tcacccgatc tttactgttg gtggctttaa tgcgctcgca gtgggaaagg aagtgagccg
     8581 gaagttcaga agtgggaacg gcttcgttgg ttaaccccca aagaatgtgg tagcccgcgc
     8641 cgtcgctgtc gacttttacg atgcctaagt tagccgcagg atcaagctgg acgttacgga
     8701 agaatttgcc ggagccggtg acaataaacg gcgtatttgc cagtaaaggc atgggctggc
     8761 tgagcggaat atagcgcggt tgttggtgga aattgtcgtg atatggtgcg atatcggcgt
     8821 catccaggcg cagagtcagg ttgccgccgt tgcgctcgtc ccagcctttc agccaggcgt
     8881 cggtggtggc tttgatcatt ccctggacaa accaggactg ggtaatgttt tgcatgttct
     8941 gtgttcctgt aaattcggtg ttgtcggatg cacgacccgt aggccggata aggc
//
LOCUS       DAPGRN010000158         8937 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPGRN010000158 DAPGRN010000000
VERSION     DAPGRN010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.160"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..200
                     /gene="hokE"
                     /locus_tag="RHW33_004217"
     CDS             48..200
                     /gene="hokE"
                     /locus_tag="RHW33_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV1647664.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFRAVL
                     VYEPKK"
     gene            complement(322..942)
                     /gene="entD"
                     /locus_tag="RHW33_004218"
     CDS             complement(322..942)
                     /gene="entD"
                     /locus_tag="RHW33_004218"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV1647665.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1117..3357)
                     /gene="fepA"
                     /locus_tag="RHW33_004219"
     CDS             complement(1117..3357)
                     /gene="fepA"
                     /locus_tag="RHW33_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV1647666.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGITWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3600..4802
                     /gene="fes"
                     /locus_tag="RHW33_004220"
     CDS             3600..4802
                     /gene="fes"
                     /locus_tag="RHW33_004220"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV1647667.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLIEQLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4805..5023
                     /gene="ybdZ"
                     /locus_tag="RHW33_004221"
     CDS             4805..5023
                     /gene="ybdZ"
                     /locus_tag="RHW33_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV1647668.1"
                     /translation="MAFSNPFDDPQGVFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5020..8901
                     /gene="entF"
                     /locus_tag="RHW33_004222"
     CDS             5020..8901
                     /gene="entF"
                     /locus_tag="RHW33_004222"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV1647669.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALRFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPVITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDSAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDIELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     VDVPDLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSVLQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPLWSIIGIQSPRPHGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSSERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
BASE COUNT         1939 a         2374 c         2462 g         2162 t
ORIGIN      
        1 gaacaatttg gatggtagtc ccttcttcgc atggaggcaa tataaacatg ctgacgaaat
       61 atgcccttgt ggcagtcata gtgctgtgtt tgacggtgct gggatttacg cttctggtcg
      121 gcgactcgct gtgtgagttt acggtgaagg aacgtaatat tgagttcagg gctgttctcg
      181 tttacgaacc gaagaagtag ccgttgtgcg gggagtaatc cccgcatatc cggttgtcag
      241 gtcagagtgg taaggcacct gctttacact ttcgcccgca gtcagtgatg gctgcgggcg
      301 aatcgtacca gatgttgtca attaatcgtg ttggcacaga gttatgacta ttttttcttt
      361 tatctgccag tgcacagcaa acatctcatt ctcacgatga atgatgacct gctgtttatt
      421 ccagctaatt atctgatagt ccagaaaacc tgcatcagtt tggatctcac ttgccttaaa
      481 tgcgctctct ttggcggaaa atgccagtgt cagcgccaga gaaaaggcta aaccgcagtc
      541 tgcgagtcgc tcgtgttctg ctggtgtaat aatgttgtct gtcaattcgg tcgctgtttg
      601 cgcagaaaaa atttcttcta tatcaatgcc aatcggttga cgagatacca cggctaatgc
      661 cgtagtccca caatggctaa tactgccgta tacctccgca ggccacacag gttggcgtag
      721 ctcgccgatt gcgggcacac atttatagcc atattcccgc aaggcataaa cagcagcgat
      781 ccgtccggct aaatgctctg ttttacgttt acgtccagcg tgttgcagtt gtgcgtagtg
      841 cggcagccag agtaaatcct gctcacaaaa attcgccgga tcgaactcaa caaaatgcag
      901 cgtatgtccg gcaaagggga gggaggtatg cgtagttttc atatcgacca tattcgagac
      961 tgatgacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacat ggtcctgcaa
     1021 tatattgaat tggcaagatt tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
     1081 aacgacgcgc actttgtcaa caatctgacg ttagcatcag aagtgagtgt ttacgctcat
     1141 ataccacgta cgtcccggct cgttataggt atacgccccg gcaccggcga tatagttggc
     1201 ccctgccaaa tcgcccgtgg tctgggcatt acccgcacgc cacaaacgtt tgtcgaacag
     1261 gttgtccacg ccgccggtca gactgacatt cttcgtcaca tcccacgtcg cgctcagccc
     1321 aacaatgctg taaggactaa tttctttggt ttccggtcca accgctggct gacctttata
     1381 gttgtacttc ttcggctgct gcttaccgta ccaggtgaag gtcgtttgca tcgacaaatc
     1441 ttctcgtgcc tgccagctca gcgttgagtt caacgtatac tccgggatga tcgacaaacg
     1501 gtcgcccgtg gttttgtttt cactcttcag catataagtg atgttattag tccacatcac
     1561 cgtttcgcta accggtacgt ttaacgatcc ttccagacct tcaaccaccg ctttcggcac
     1621 gttatcccac tgatagagat cggtgccgac tgcgttttgc cctacagcaa catagcctgc
     1681 ttcaatctta ttgcgataat cgttacggaa ccaggtgatg cccgccagcc agccgtcgcg
     1741 tttgaactcc agaccaatct ctttgttgat gctggtttct gctttcaggt catcgttacc
     1801 ttgcagatag cagccgcccg cgctggcata gcagccctga cctttactgt agagaatgta
     1861 gttcgggtta gtctggtaca ggctcggcgc tttataggcg cgggcaatgc ccattttcag
     1921 cgtgaagtca tcgcctaaac cttgcgatat gttcagcgcc gggctccagt tattgccgac
     1981 aatactgtga tgatcgaaac gcagccccgg cgttacgatg gtgctgtcag tcagctccat
     2041 gttgttttcg gcaaacagcg agaaaatttc tgcttttgaa tacgggctac ggtcggtggc
     2101 actcacacca tcaatagcgc cgccggtgtt cgtcccggtc agcgcctggg tattggaact
     2161 taagtccttc atccgttgct gattccactc cgtacccagc gtcagcgtct ggttaacgag
     2221 gaaatcaatc ggcaggttaa cttcgctatg cagcatcacg tcatcaagat cgatatcgac
     2281 gaaatcctgt gtcgcttttt cgttaaattt cccttcggta ccgcccgcca gaccttccgg
     2341 aatacgcgag ttacgggtgt gttcgtactg cacccagttg ctggtggtca cgccgttatc
     2401 ccagccaccg ttccaggtca gcgagtagtt ctggcgatac aggcggttgg tttcatcgcc
     2461 atatttcgag cgagtgtaag cgtcagagtt ggtgttctgg gtatcgcccg catacaggtt
     2521 accctggcgg ctgtaacccg cttccagttc gagtgattgc agaggcgcga agtcccagcg
     2581 caccacgcca ttaatatctt tgttgatcac cccttcgcgc ccggctggta acgtcgtggc
     2641 atacgttccg gcacgcgcgg actgatggcc ctggttgata tcccacgcgt cagcctgggt
     2701 tttgtcgagg ttgccataca agcggaagct gaattcgtcg cccagcggac cggtcaggct
     2761 gaagttggtg cgtttagtgg caccttcctc tttatgttct ggcgcattga aataagcgtc
     2821 ccaggagccg tgccactcgc cgctgccttt tttggtaatg atgttaacca cgccgcccgc
     2881 cgcgccgttg ccataacgcg cagctgccgg accacgcaga acttcaatac gttcaatcat
     2941 ttcaggtggc acccaggagg tatcaccacg ggtatcgcgc tcgccacgcc agccctgacg
     3001 caccgagtta cggctgctta ccggcttgcc gtcaatcaaa atcagcgtgt tttccggacc
     3061 cataccgcga atatcaatct gtcggttatt cccacgctga ccactggtgg agttaccggt
     3121 caggttaacg cctggcatgg tacggatgat ctccgacaca tcgcgggcaa cggggttttt
     3181 gcggatttca tctgcggtga tggtcgaaac gccaggcgcc tgtaagttct gctcggcggc
     3241 ggtaacgaca atagtatcgt catgtgaaac aggagtatcg gtcggctctt gtgcctgcgc
     3301 taccccataa atccccagat tgaccaacaa ggccagggaa tgaatcttct tgttcattgt
     3361 tttattcctg catttttgcc acgaattgca actgtcgggc atggtcgtca tcaacacgac
     3421 gcatcccgct accgcgaaaa cctttgatcc tgaaagacac gcagtgcagt tggttaatta
     3481 atgtccgcgc ttcccacggc gcgccattac gctattgcaa atgcaaatag ttatcaataa
     3541 tattatcaat atatttctgc aatcaatgaa aaattgcaca gtaaacatgg ggttatggtg
     3601 tgacggcgtt aaaagtagga agtgagagct ggtggcagtc gaaacatggc ccggaatggc
     3661 agcgtctgaa tgacgaaatg tttgaggtca ctttctggtg gcgtgatccc caaggttctg
     3721 aagaatactc gacgataaag cgcgtatgga tctacatcac tggtgtgacc gatcatcatc
     3781 agaacagcca gccccagtcg atgcagcgaa ttgcaggtac caacgtctgg cagtggacga
     3841 cacaactcaa tgccaactgg cgcggcagct actgctttat tcccaccgaa cgcgatgaca
     3901 ttttttctgc accatccccc gatcgcctcg aattgcgcga aggctggcga aaactattac
     3961 cccaggcgat agccgatccg ctgaatccac aaagctggaa aggcgggcga gggcacgctg
     4021 tttctgcact cgaaatgccg caagcgcctc tgcaaccggg atgggattgt ccgcaagcgc
     4081 cagaaatacc agccaaagaa attatctgga aaagtgaacg gttgaaaaat tcacgtcgtg
     4141 tatggatttt taccaccggc gatgtaacag cagaagaacg cccgctggca gttttgctcg
     4201 atggcgaatt ttgggcgcaa agtatgcccg tctggcctgt gctgacttcg ctgacccatc
     4261 gtcagcaact tcctcccgcc gtgtatgtgt tgatcgacgc tatcgacacc acgcaccgcg
     4321 cccacgaact gccgtgtaat gcggatttct ggctggcagt acagcaagag ttattgcccc
     4381 tggtgaaagc tattgcccct tttagcgatc gcgccgatcg caccgtggtc gccgggcaga
     4441 gttttggtgg gctttccgca ctgtatgccg gactgcactg gcctgaacgc tttggctgtg
     4501 tattaagcca gtcaggatcg tactggtggc cgcatcgggg cgggcagcaa gaaggtgttt
     4561 taattgagca acttaaagct ggtgaagtta gcgccgaagg tctgcgcatt gtgctggagg
     4621 cgggtattcg cgagccgatg atcatgcggg ccaatcaggc gctgtatgcg caattacacc
     4681 ctataaaaga atccattttc tggcgtcagg ttgacggcgg acatgatgcg ctttgttggc
     4741 gcggtggctt gatgcagggg ttaatcgacc tctggcaacc acttttccat gacaggagtt
     4801 gaatatggca ttcagtaatc ccttcgatga tccacaggga gtgttttaca ttttgcgcaa
     4861 tgcgcagggg caattcagtc tgtggccgca acaatgcgcc ttaccggcag gctgggacat
     4921 tgtgtgtcag ccgcagtcac aagagtcctg ccagcagtgg cttgaagccc actggcgtac
     4981 tctgacaccg gcgaatttca cccagttgca ggaggcacaa tgagccagca tttacctttg
     5041 gtcgccgcac agcccggcat ctggatggca gaaaaactgt cggatttacc ctccgcctgg
     5101 agcgtggcgc attacgttga gttaaccgga gaggttgatg cgccattact ggcccgcgcg
     5161 gtggttgccg gattagcgca agcagatacg ttgcggatgc gttttacgga agataacggc
     5221 gaagtctggc aatgggtcga tgatgcgctg aggttcgaac tgccagaaat tatcgacctg
     5281 cgaaccaaca ttgatccgca cggtactgcg cgggcattaa tgcaggcgga tttgcaacaa
     5341 gatttgcgcg tcgatagcgg taaaccactg gtctttcatc agctgataca tgttgcggat
     5401 aaccgctggt actggtatca gcgttatcac catttgctgg tcgatggctt cagtttcccg
     5461 gtcattaccc gccagatcgc caacatttac tgcgcattgc tgcgtggcga accaacgcct
     5521 gcttcgccgt ttacgccttt cgctgatgta gtggaagagt accagcaata ccgcgaaagc
     5581 gaagcctggc agcgtgattc ggcattctgg gtagaacagc gtcgtcaact gccgccgccc
     5641 gcgtcacttt ctccggcacc tttagcgggg cgcagcgcct cggcagatat tctgcgcctg
     5701 aaactggaat ttaccgacgg ggaattccgc cagctggcta cgcaactttc aggtgtgcag
     5761 cgtaccgatt tagcccttgc gctggcagcc ttgtggctgg ggcgattgtg caatcgcatg
     5821 gactacgccg ccggatttat ctttatgcgt cgactgggct cggcggcgct gacggctacc
     5881 ggacccgtgc tcaacgtttt accgttgggt attcacattg cggcgcaaga aacgctgccg
     5941 gaactggcaa cccgactggc agcacaactg aaaaaaatgc gtcgccatca acgttacgat
     6001 gccgaacaaa ttgtccgtga cagtgggcga gcggcaggtg atgaaccgct gtttggtccg
     6061 gtactcaata tcaaggtatt tgattaccaa ctggacattc ctgatgttca ggcgcaaacc
     6121 cataccctgg caaccgggcc ggttaatgac attgaactgg ccctgttccc ggatgaacac
     6181 ggtgatttga gtattgagat cctcgccaat aaacagcgtt acgatgagcc aacgttaatc
     6241 cagcatgctg aacgcctgaa aatgctgatc gcccaattcg ccgcggatcc ggcgctgttg
     6301 tgcggcgatg tcgatattat gctgccaggt gagtatgcgc agctggcgca gatcaatgcc
     6361 actcaggttg agattccaga aaccacgctt agcgcgctgg tggcagaaca agcggcaaaa
     6421 acaccggatg ctccggcgct ggcagatgcg cgttaccagt tcagctatcg ggaaatgcgc
     6481 gagcaggtgg tggcgctggc gaatctgctg cgtgagcgcg gcgttaaacc aggggacagc
     6541 gtggcggtgg cattaccgcg ctcggtcttt ttgaccctgg cactccatgc gatagttgaa
     6601 gctggcgcgg cctggctacc gctggatacc ggctatccgg acgatcgcct gaaaatgatg
     6661 ctggaagatg cgcgtccgtc gctgttaatc accaccgacg atcaactgcc gcgctttgtt
     6721 gatgttccag atttaaccag cctttgctat aacgccccgc ttacaccgca gggcagtgcg
     6781 ccgctgcaac tttcacaacc gcatcacacg gcttatatca tctttacctc tggctccacc
     6841 ggcaggccga aaggggtaat ggtcgggcag acggctatcg tcaaccgcct gttgtggatg
     6901 caaaatcatt atccgctcac aggcgaagat gtcgttgccc aaaaaacgcc gtgcagtttt
     6961 gatgtctcgg tgtgggagtt tttctggccg tttattgccg gggcgaaact ggtgatggct
     7021 gaaccggaag cgcaccgcga cccgctcgct atgcagcaat tctttgccga atatggcgta
     7081 acgaccacgc actttgtgcc gtcgatgctg gcggcatttg ttgcatcgct gactccgcaa
     7141 accgctcgcc agaattgcgc gacgttgaaa caggttttct gtagtggtga ggccttaccg
     7201 gctgatttat gccgcgaatg gcaacagtta acgggcgcgc cgttgcataa tctatatggc
     7261 ccgacggaag cggcggtaga tgtgagctgg tatccggctt ttggcgagga actggcacag
     7321 gtgcgcggca gcagtgtgcc gattggttat ccggtgtgga atacgggctt gcgcattctc
     7381 gatgcgatga tgcatccggt gccgccgggt gtggcgggag atctctatct caccggtatt
     7441 caactggcgc aggggtatct tggacgaccc gatctgaccg ccagccgctt tattgccgat
     7501 ccttttgtcc ctggtgaacg gatgtaccgt accggagacg ttgcccgctg gctggataac
     7561 ggcgcggtgg agtacctcgg gcgcagtgac gatcagctaa aaattcgcgg gcagcgtatt
     7621 gaactgggcg aaatcgatcg cgtgatgcag gcgctgccgg atgtcgaaca agccgttacc
     7681 cacgcctgtg tgattaacca ggcggcagcc accggtggtg atgcgcgtca gttggtgggc
     7741 tatctggtgt cgcaatcagg tctgccgttg gataccagcg tattacaggc acagcttcgc
     7801 gaaacattgc cgccgcatat ggtgccggtc gttctgctgc aacttccaca gttaccactt
     7861 agcgccaacg gcaagctgga tcgcaaagcc ttaccgttgc ctgaactaaa ggcacaagcg
     7921 ccagggcgtg cgccgaaagc gggcagtgaa acgattatcg ccgcggcatt ctcgtcgtta
     7981 ctgggttgtg acgtgcagga tgccgatgct gatttcttcg cgcttggcgg tcattcgcta
     8041 ctggcaatga aactggcagc gcagttaagt cggcaggttg cccgccaggt gacgccgggg
     8101 caggtgatgg tcgcgtcaac cgtcgccaaa ctggcaacga ttattgatgg tgaagaggac
     8161 agctcccggc gcatgggatt cgaaaccatt ctgccgttgc gtgaaggtaa tggcccgacg
     8221 ctgttttgtt tccatccggc atccggtttt gcctggcagt tcagcgtgct ctcgcgttat
     8281 ctcgatccac tatggtcgat tatcggcatt cagtcgccgc gccctcatgg ccccatgcat
     8341 accgcggcaa atctggatga agtctgtgaa gcgcatctgg caacgttact tgaacaacaa
     8401 ccgcacggcc cttattacct gctgggttat tcacttggcg gtacgctggc gcagggtatt
     8461 gcggcgcggc tgcgtgcccg tggcgaacag gtggcatttc ttggcttgct ggatacctgg
     8521 ccgccagaaa cgcaaaactg gcaggaaaaa gaagctaatg gtctggaccc ggaagtgctg
     8581 gcggagatta accgcgagcg cgaggccttc ctggcggcac agcagggaag tacttcaacg
     8641 gagttgttta ccaccattga aggcaactac gctgatgctg tgcgcctgct gacgactgct
     8701 catagcgtac cgtttgacgg taaagcgacg ttgtttgttg ctgaacgcac gcttcaggaa
     8761 ggtatgagtt ctgaacgcgc ctggtcgccg tggattgcgg agctggatat ctatcgtcag
     8821 gattgtgcgc atgtggatat tatctctcca ggggcatttg aaaaaattgg gccgattatt
     8881 cgcgcaacgc taaacagata aattaacatt atctataaac ccataattgc aggaaat
//
LOCUS       DAPGRN010000159         8841 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPGRN010000159 DAPGRN010000000
VERSION     DAPGRN010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.161"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1707
                     /gene="espL4"
                     /locus_tag="RHW33_004223"
     CDS             <1..1707
                     /gene="espL4"
                     /locus_tag="RHW33_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HDV1647670.1"
                     /translation="QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSR
                     PEGRCVAACGDFGLVMKAYFDKMESNDLSVMAAILLVDNHALTVRLRIKNTTEGCIHY
                     VVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPAL
                     PKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLKQLMIQLQKMPELHRTEMLTAY
                     NSINLPGLYLAINYGNADIVETIFNSLSEPGYEGLLSKKNLMHILEAKDKNGFSGLFL
                     AISRKDKNVVTSILNALPKLAATHHLDNEQVYKFLSAKNSTSSHVLYHVMANGDADML
                     KIVLNALPLLIRTCHLTKEQVLDLLKAKDFYGCPGLYPAMQNGHSDIVKVILEALPSL
                     AQEINISASDIVDLLTAKSLARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKN
                     MKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMGFWQHKAP
                     QKYSAFELACELGHRVIAELIFNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHT
                     VR"
     gene            complement(1676..3412)
                     /gene="aceK"
                     /locus_tag="RHW33_004224"
     CDS             complement(1676..3412)
                     /gene="aceK"
                     /locus_tag="RHW33_004224"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV1647671.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAEFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGPENLSKSHLQVANELFYRNKAAWLVGKLITPSG
                     KLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEDMHADLFRADYWRTLQNRIRAGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3596..4900)
                     /gene="aceA"
                     /locus_tag="RHW33_004225"
     CDS             complement(3596..4900)
                     /gene="aceA"
                     /locus_tag="RHW33_004225"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV1647672.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDNTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4930..6531)
                     /gene="aceB"
                     /locus_tag="RHW33_004226"
     CDS             complement(4930..6531)
                     /gene="aceB"
                     /locus_tag="RHW33_004226"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV1647673.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQNIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITANQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6800..7729)
                     /gene="metA"
                     /locus_tag="RHW33_004227"
     CDS             complement(6800..7729)
                     /gene="metA"
                     /locus_tag="RHW33_004227"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV1647674.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLKPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7886..8329
                     /locus_tag="RHW33_004228"
     CDS             7886..8329
                     /locus_tag="RHW33_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV1647675.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVGHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(8392..8775)
                     /locus_tag="RHW33_004229"
     CDS             complement(8392..8775)
                     /locus_tag="RHW33_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647676.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSAVLSDSAVMAMLKQK"
BASE COUNT         2254 a         2152 c         2179 g         2256 t
ORIGIN      
        1 caatatatta atgatattta tcagaatgtt gactacaaac cccatcaaga tgattactct
       61 tcagctgaaa aatttctcac tcacttcaac aaaaaatgca aaaaccagac tttggcgttg
      121 atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg gctagttatg
      181 aaggcatatt ttgacaagat ggaatcaaat gacctcagtg ttatggctgc catattattg
      241 gtagataacc atgctttgac ggtccggcta agaataaaga acacaactga aggatgtatc
      301 cattacgtgg tttcggttta tgatcctaat gtaactaacg ataaaataag aatcatgagc
      361 gaaagtaaag aggatattaa acactattca ctgatggatt ttatgaatgt agattatagc
      421 ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat tccagcactt
      481 ccaaaagaac agctattgat gctaaaagga tctgtggatg aaataacccc tccattatca
      541 cctgcaacga tgaatttgct aatggcaatt ggtcagaatc accaacttaa gcaactgatg
      601 attcaactcc agaaaatgcc agaactacat agaacagaaa tgttgactgc ctataatagt
      661 attaacctcc ccggtttata tttggctata aattatggta atgcggatat cgttgagact
      721 atttttaatt cattgtcaga gccaggatat gaaggattac tctcgaaaaa aaatctcatg
      781 catattctgg aggcaaaaga taaaaatggt ttttctggat tatttttagc gatatcacgt
      841 aaggataaaa atgttgtaac ctcgattctg aacgccttac ctaaactggc cgcaacacat
      901 catttagata acgaacaagt gtataaattc ctgagtgcca aaaatagtac gtccagccat
      961 gttttatacc atgttatggc gaatggtgat gccgacatgc tgaaaattgt tttgaacgcg
     1021 ttacctttgt taattcgcac atgtcatttg actaaagaac aggtactcga tctcctgaag
     1081 gcaaaggatt tttatggttg cccaggacta tacccggcga tgcaaaatgg acatagcgat
     1141 atcgtgaaag ttattcttga agcattgccc agcctggccc aggaaattaa catttcagct
     1201 tccgatattg tagatcttct gaccgctaaa agccttgcgc gcgacacggg tttgtttatg
     1261 gccatgcagc gcggacatat gaacgttatt aatactattt ttaacgcatt acccactctg
     1321 tttaacacgt ttaaattcga taaaaaaaat atgaagcccc tcctcctggc aaataattct
     1381 aatgaatatc ccggtttgtt ttcagcgata cagcataaac aacaaaacgt tgtagagacg
     1441 gtttatcttg ctttatctga ccatgcacgc ctgtttggat ttaccgctga agatattatg
     1501 ggtttttggc aacacaaagc gccacaaaaa tactctgcct ttgagttggc ttgtgaattg
     1561 ggtcaccggg ttattgctga attaatcttt aatacattaa ataagatggc tgaaagcttt
     1621 ggctttacgg ataaccctcg atacattgcg gagaaaaatt atatggaagc tttactcaaa
     1681 aaagcatctc cccataccgt acgctaaatc tttgcctgcg ccgatacgca taaacatctt
     1741 ccacatgtcc tgcacgaatg cggttttgta gtgtgcgcca gtaatcagcg cggaacaggt
     1801 cggcgtgcat atcttcaaag agtggcccaa tgcgggggtc ggcgcatagc cagtggcgga
     1861 actcttccgg gaaaacatcg cccggcgaga cgctgtacca cggttcgctg gcaagttcgt
     1921 cttccggata gcgcggcggc gggatgtcgc ggaagttcac ttccgtcatg tagcaaattt
     1981 catcgtaatc ataaaaaacc acacgcccgt gacgggtgac accgaagttt ttaaacagca
     2041 tgtcgcccgg gaaaatgtta gcagcggcaa gctggcgaat ggcattgccg tactcttcaa
     2101 tggcgtcgcg caactgctga ccttccactt gttccagcca gatattgagc ggcaccatcc
     2161 gccgctcaat ataaagatgg cgaatcacaa tttgttcgcc gagatcggtg attttttccg
     2221 ctgcttcctg gagcagtaat tccattaatg ccggggaaat atgccgcttc tccagcacaa
     2281 agttttcaaa ctcctgggtg tccgccattc ggcccacgcg atcgtgctct ttcaccagtt
     2341 gatagcaggc acgaacgtga gcggcagaca tctctttctg cggcgcgaac ctgtctttga
     2401 tgactttaaa tacacggtca aagcccggca gcgtgaacac caacatcacc atgccacgaa
     2461 tacccggcgc ttcaatgaac tgctcattac agccctgtag ataaacgaga tattcgcggt
     2521 agctttcggt tttggcgtgc ttctggcagc cgatagccat atacaattca gcggtggttt
     2581 tacctggcag aatttcccgt aaccactcga ccagcgctgc gggcagcggc gcataaacca
     2641 taaaataaga acgcgcaaag ccaaaaacaa tgctcgcttc ggcggtcgtc gtcagacagg
     2701 tatcaataaa cagttcgccg tcatccgtct ggtggatcgg caataaaaat ggcaacttgc
     2761 cggaaggcgt tattagtttg cccaccagcc aggcggcttt attacggtaa aacagttcgt
     2821 tcgccacctg taaatgactt ttggagaggt tttctggccc caacgtttcc gtcagatggc
     2881 gaataatgta atggatatca cggcttttat tctgccagcg caggcgcagc ggcaggtcgc
     2941 tgataacgcg catcagtaga gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg
     3001 ggcggggaat ggtacgaaag cggcgctctg gctgagagct aaagataaaa agccgctcgg
     3061 gagtaagcga gcggtggtca aataaccgac agtacacgga gttaaaaaag ctctccgcaa
     3121 tctcgaagcg cgggtaatcc ggcaacagcc gggtgtaatg ctctttgacg cgcagtaaaa
     3181 attccgcgtc cgtgctttgg ccgttggtaa tgcagcgcag ttgctccacg accagaccaa
     3241 cgtgatgatc gtaaagatgg atacggtttt tcattgcctg ctggacagca tgccagtcgg
     3301 cctgttcgaa acgctgctgc gccccggaag tcacttcgag gaatcgacca tactgagcgt
     3361 cgaagccttg caaaatggtt tgagcaatca gtaattccag gccacgcggc atttagcgcc
     3421 ctcatcagga gcagagaatt gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg
     3481 caacggttgt aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc
     3541 cggatgcggc gtaaatgcct tatccggcct acagtcagca acgtttgtag gttgcttaga
     3601 actgcgattc ttcagtggag ccagtcagcg cggtaaccga cgacgtgccg ccctgaataa
     3661 tggtcgtcac tttatcgaag taacctgtac ccacttcctg ctggtgagat acgaaggtgt
     3721 agccatcttt cgcggcggcg aattccggct gctgcacttt ctcaacatag tgcttcatac
     3781 cctcgccctg ggcataggcg tttgccaggt caaacatgtt gaaccacatg ctgtggatac
     3841 ctgccagggt gatgaactgg aacttgtagc ccatatccga cagctgctgc tggaagctgg
     3901 caatggtgtt gtcgtcgagg tttttctgcc agttgaacga cggcgagcag ttataagcca
     3961 gcagtttgcc cggatatttc gcgtggatgg cttgtgcaaa gcgacgcgcc agttccagat
     4021 ccggcgtgga ggtttcacac catacaaggt cagcatacgg cgcatacgcc aggccacggc
     4081 tgatcgcttg ctcaatgccc gcatgagtac ggaagaagcc ttcactggta cgctcgccgg
     4141 taataaattc gctgtcatac gggtcgcaat cggaggtaat cagatccgcc gcatcagcat
     4201 cggtacgagc aaccagcagg gttggaacgc ccgtaacatc agctgccaga cgcgccgcga
     4261 ccagtttctg aatagcttcc tgagttggca ctaaaacttt gccgcccatg tgaccgcatt
     4321 tcttcactga tgccagctga tcttcgaagt gaactgccgc tgcaccggct tcaatcatcg
     4381 ctttcatcag ttcaaaggca ttcaggacac cgccaaaacc ggcttccgca tcggcaacga
     4441 tcggcaggaa gtaatcgaca tagcgcggat cgcccggctc aatgcccgcg gaccattgga
     4501 tctgatccgc acggcggaaa gtgttgttga tccgctccac cacagctggc accgagtttg
     4561 ccggatagag cgactgatcc ggatacatgc tggccgccag gttagcgtcc gccgctacct
     4621 gccatcccga cagatagacc gcttcaatac ccgctttcgc ctgttgcagc gcctgaccgc
     4681 cagtcagtgc accgaggctg ttgatgtagc cttttttcga ctcgccatgc agcagacgcc
     4741 acattttcgc cgcgcccagt tgcgccagcg tgcattcagg attgactgaa ccgcgtaatt
     4801 tcaccacatc ttccgcgctg tatgggcgag taattccttc ccaacgcggt tgagtccact
     4861 ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc tccatagtta
     4921 tgtggtggtt tacgctaaca ggcggtagcc tggcagggtc aggaaatcaa ttaactcatc
     4981 ggaagtggtg atctgttcca tcaagcgtgc ggcatcgtca aaacgcccct gggagaaacg
     5041 ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct ggcggaacaa
     5101 ggctttggtc accggtttgc cattgctcaa cgttttttga tgatggatcc actgccagat
     5161 cgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa tcggcacgca
     5221 gccgttgccg gagatccacg cttcgatgta ctgcacagcc acgcgaatgt tggcgcgcat
     5281 accttcttcg gtgcgttcac catcacaagg tgccagtagc tgattggcag taatcggcgc
     5341 gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt cgttgaatac
     5401 cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt gaccgttgtt
     5461 ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt gctcttcatc
     5521 tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt tatggcaggt
     5581 tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtta ctgcctgtct
     5641 gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga agatgtaatc
     5701 ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga tttcatccat
     5761 ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc cgcgcggcag
     5821 attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt cctgccagga
     5881 ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagtg cctgatagtt
     5941 gtggaagaaa tagagcgcaa aatcaaacag gctaccgggg attgcctcac cacgccaggt
     6001 gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg ctggattggg
     6061 cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtgccgt taactgcatc
     6121 acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg aatcttcgaa
     6181 atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct tgcgctctac
     6241 cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc gaattttcca
     6301 gtcagcatca cgaatggaag ctgtttccga aataaaatca ggcaacgttc cgttatcaat
     6361 attttgctgc tgctgaatgc gcgctgccag aagtttattg cgttgtggcg taaaatgcgt
     6421 caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct cctgctcgcc
     6481 atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca tcgtgcagct
     6541 cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg cgcttttcgt
     6601 tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc cattttaatt
     6661 aaaaatactt gataacttat tgataacaat atgaataaaa tttattcacc tcctgaggtg
     6721 cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga atcgcttaac
     6781 gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga tcgtatggcg
     6841 tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg tgactacgcc
     6901 agctcgctcg cggtttgttt tgcggatcat tgtgcgggaa atagttatac ggtacatccg
     6961 gttttagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc gcatcatatt
     7021 cgggatggcc cgtcacaaag gcaatgcgct tatctttact ggcaaacaga tatgcatccc
     7081 cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac gctgccggaa
     7141 agtcagcata gcgcgaatgc ggtgccagga atgaatcatc aaagccacgc gtcagaagcg
     7201 catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg cgagtttgct
     7261 taggaatgcc gtagaggata ttgagcgctg cctgtaccgc ccagcagaca aacagcgtcg
     7321 aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag taagcgacat
     7381 cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca aagttctgtt
     7441 cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc gtgttgcgcg
     7501 attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag tttgaaagca
     7561 ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg atcagaacct
     7621 taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag acgttttctt
     7681 cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata acctgattac
     7741 ctcactacat acgcttatac gtttagacat ccagatagct gaagataacc agaaaatttg
     7801 ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact acgttagaat
     7861 ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac ggcatgagga
     7921 aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacccacg attttttatc
     7981 agcagagtat cgggccgagc tggaagaact ggttcgttcc tttctgccgg aagcaccgtt
     8041 gtgggtcgcg gttaatgaac gggatcagcc ggttgggttt atgttgttaa gtgggcagca
     8101 tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc ggcgtgggtc ggatgctggt
     8161 ggggcatgcg ctctcgatgg caccggaact gacaaccaac gttaatgagc aaaatgagca
     8221 ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg aggtggacga
     8281 tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggcgtagg tgattttttc
     8341 tcatcccggt gggctgaact tcaggtttcg tacccaccaa tggtgacacg attatttctg
     8401 cttaagcatt gccatgaccg cactatcact gagtaccgct gattgcgcat ggatgtggaa
     8461 ctggcgatat ttcattaacg ttgccccagc gaccacttca tggagaatgc gttcctcaac
     8521 ctgtgacaaa ccgccctcat tcatttccag cgcgctcaca acaatatcca tacgttttgc
     8581 tggcaaaaaa gaatgccagg ttgaacgtgg gtggagttgc agaacaagat cttgcagaac
     8641 tttttctgca acaaagaaat tggcgattaa caggcgctgg ttactgaagg cagcgtctcc
     8701 tgacacttca cgtttgcttt caagatcgcg aacagtaatt tgattacgac gaatttggat
     8761 atacagaact gacatgagat tcccttcatc atgcaaataa ttgatatgca aaaaaccccg
     8821 gccttatgga ccggggtttt t
//
LOCUS       DAPGRN010000160         8780 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPGRN010000160 DAPGRN010000000
VERSION     DAPGRN010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.162"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(456..1301)
                     /gene="sseA"
                     /locus_tag="RHW33_004230"
     CDS             complement(456..1301)
                     /gene="sseA"
                     /locus_tag="RHW33_004230"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV1647677.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEVVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPLK"
     gene            1508..6469
                     /gene="yfhM"
                     /locus_tag="RHW33_004231"
     CDS             1508..6469
                     /gene="yfhM"
                     /locus_tag="RHW33_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV1647678.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPCVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDTTRGEEAIVLALKTRRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            6470..>8780
                     /gene="pbpC"
                     /locus_tag="RHW33_004232"
     CDS             6470..>8780
                     /gene="pbpC"
                     /locus_tag="RHW33_004232"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311412.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV1647679.1"
                     /translation="MPRLLTKRGCWITLAATPFLLFLAAWGADKIWPLPLHEVNPARV
                     VVAQDGTPLWRFADAEGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPTNLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKITTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGEIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVHFSFQ"
BASE COUNT         2069 a         2219 c         2469 g         2023 t
ORIGIN      
        1 tgaaggttgt taaccaccag taaattgttg ccggagagac tcaggttatt atttacctcg
       61 gttaacagcg caccagaaac aataagtacg atacagaagc accgtatctt cattgtgcgg
      121 tccatgcgaa gaccgcatca cgacagcacc actggcgata ttgccgcgat agcaagcact
      181 gtggggtgcc tgtatttacg gtcatggaac atcctttcct aaagccagcg cccgtttgcg
      241 agacggcgaa aagggccatt aagaataata ctctcatgtt tgaataatta ctctgtttat
      301 tatcttgaca cagccaggca taaaagagag tatttcaaca ctgtggggtg cctgcgttgc
      361 tccggcaaca caagtcaacc tgctggaaag gccgcgaacc agaccagcaa taaaaaaccg
      421 ccaaattcgg cggtttttta ttgcctgtaa aaagcttatt tcaatggctc aaccggtaaa
      481 tctgcccgcg cgccccattc actccatgcg ccgtcgtaca gtttcacgtt tggcacatcc
      541 agcgtcgcga gtgccaacaa aaccacggct gctgttacac cagagccgca gctgacgata
      601 atcggtttgt catagctgac gccgcgacca aaaaatatcg catccagttc atcggtcgtt
      661 tttagttcgc cttcgcgcac cagttccgtc cacggaacgt tcaatgcacc gggaatatgt
      721 ccgcgacgta aacctgggcg aggttcatca acttctgcgt taaaacgtgc agccgggcgg
      781 gcatcaataa tttgcgccgt attttcatgg cttgccaata acacatcggt tactttcacc
      841 actacttcag gattaaacgc ggcgttaaac tctccttccg gcagctctac tgcaccttct
      901 tctaacaaca gatcatcgcg ctgccagcct gcaagtccac cccccagaat cgacactttc
      961 tctacaccaa aggtgcgcag catccaccat gctcgtgggg ctgagaaaag attaccttcg
     1021 tcatagacaa tcaggtgctt atcctggtta acgcctaatt cacgcatcgc cacggcgaac
     1081 gtttccgggc gcggcagcat gtgcggaagc ggggaagtgt gatcagaaag tgcttcgata
     1141 tcaaaaaaca ctgcgccggg aatatgtcca ttcagatact cctgagcaac gttacgatcc
     1201 tcctgtccag gcgacgccat gcgggcatcg ataatctgaa tttccgggtc atcaatatgt
     1261 tcggcgagcc agtcggctcc tacaaaccat gtcgtggaca taggcatctc catttttgcc
     1321 ggggttaggc taaattgtcg gatgtttagt gaaaagcgac aagcaaaaga ggctgatttg
     1381 gctgtctgag ccacaattct cactaaaatt cggattgtat gggcgtgata ctgaattaca
     1441 taacgtaaat tctcacgcat aataattatt cgacgttgct aacgggccat tgtggcaggg
     1501 atgaaaaatg aaaaagttac gcgtagccgc ctgcatgcta atgctggcgc tggcagggtg
     1561 cgacaacaac gataacgcgc caacagcggt gaaaaaagat gcgccttctg aagttactaa
     1621 agcggcctct tcagaaaacg cgagttcagc aaaactctcc gtgccggagc gacaaaaact
     1681 ggcccaacag agtgccggta aggtgctgac attgctggat ctctctgaag tccaacttga
     1741 tggtgcagcc acgctggtgc tgacgttctc catccctctc gacccggatc aggatttctc
     1801 acgcgttatt catgtcgtcg ataaaaaaag cggcaaagtg gatggtgcct gggagctgtc
     1861 agataatctt aaagagctgc gtttacgcca cctcgaaccg aaacgtgatt tgatcgttac
     1921 tattggcaag gaggtcaaag cactcaacaa cgcaaccttc agtaaagatt acgaaaaaac
     1981 tataactacc cgcgacatcc aaccctgcgt cggttttgcc agccgtggtt cgctgctgcc
     2041 aggtaaagtc gttgaagggc tgccggtaat ggcgctcaac gttaataatg tcgatgttaa
     2101 cttcttccgc gttaagccag aatctctgcc agcattcatt agccaatggg aataccgcaa
     2161 ttcgctggcg aactggcagt cagacaaact gctgcagatg gcggatctgg tctacaccgg
     2221 acggtttgat ctcaatcctg cgcgtaacac ccgtgaaaaa ttattgctgc cgctgggcga
     2281 tatcaaaccg cttcagcagg cgggcgtgta tctggctgtg atgaatcagg ctggacgtta
     2341 cgattacagt aatcccgcga cgctgtttac gttaagtgat atcggcgttt cagctcaccg
     2401 ttatcacaat cgtctggata tctttaccca aagtctggaa aacggcgcgg cccagcaagg
     2461 aattgaagtc tctttattaa atgagaaagg gcagactctg actcaggcaa ccagtgacgc
     2521 tcaggggcat gtgcagctgg aaaatgataa aaacgccgca ttattgctgg cacgtaaaga
     2581 cggtcagaca acgctactcg atttaaaact tccggcgctg gacttagcag aatttaacat
     2641 tgctggcgcg ccaggctata gcaaacagtt tttcatgttt ggcccgcgcg atctttatcg
     2701 cccgggtgaa acggtaatcc tcaatggttt gctgcgtgat gcagacggta aagcgttgcc
     2761 caatcaaccc atcaagttag acgtgattaa acccgatggg caggtactca ggagcgtcgt
     2821 tagtcagccg gaaaatggcc tctaccactt tacctggcca ctcgatagca atgcggcaac
     2881 cggtatgtgg catattcgcg ctaacacggg cgataaccag tatcggatgt gggatttcca
     2941 cgtcgaagat tttatgccag agcgtatggc gctgaatctg accggtgaga aaaccccgct
     3001 tacgccgaat gatgaagtga aattctccgt ggtgggatac tacctgtacg gtgcgcctgc
     3061 taatggtaat actttgcaag ggcaactttt cctgcgccca ctgcgtgagg ctgtgtcagc
     3121 cttacctggt tttgaattcg gcgatatagc tgccgaaaac ctttcccgca cgctggatga
     3181 agttcagttg acgctggacg ataaagggcg cggcgaagtt tctacagaaa gccagtggaa
     3241 ggaaacgcat tccccattgc aggttatttt ccagggcagt ttgctggaat cgggcggtcg
     3301 cccggtgacg cgccgcgctg agcaggctat ctggcctgcc gatgcattgc cggggctccg
     3361 tccgcagttc gcctcgaaat cggtttacga ttatcgcact gacagcacgg tgaaacagcc
     3421 cattgttgat gagggcagta acgccgcttt tgacatcgtt tatagcgatg cgcaaggcgt
     3481 gaaaaaagcc gtgtcgggct tgcaggtgcg cctgattcgc gaacgccgcg attactactg
     3541 gaactggtca gaagatgaag gctggcagtc acagtttgat caaaaagatc tgatcgaaaa
     3601 tgaacaaact ctggatctga aagcggacga aaccggcaag gtcagttttc cggtagagtg
     3661 gggcgcttat cgtctggaag tcaaagcgcc gaatgaagcg gtcagtagtg tgcgtttctg
     3721 ggctggctat agctggcagg acaacagcga cggaagcggt gccgtgcgac ccgaccgtgt
     3781 cacgctgaaa ctggataaag ccagttatcg ccctggcgat accattaagt tgcatattgc
     3841 cgcgccaacg gcgggtaaag gttatgcgat ggtcgagtcc agtgaagggc cgctgtggtg
     3901 gcaagagatt gatgttccgg ctcaagggct ggatctgacg attccggtcg ataaaacctg
     3961 gaatcgtcat gatctttatt tgagtacgct ggtggtgcgt cctggcgata aatctcgctc
     4021 cgcgacgcca aaacgcgcgg ttggtgtgtt gcatctgccg cttggtgatg aaaaccgtcg
     4081 tctcgatctg gcgctggaaa caccagcaaa aatgcgtccc aatcaaccat taaccgtgaa
     4141 aattaaagcc agcactaaaa atggcgagaa gcctaaacag gtgaatgtgc tggtgtctgc
     4201 cgttgatagt ggtgtgctga atattactga ctacgtcacg ccagatccgt ggcaggcgtt
     4261 ctttggtcag aaacgctatg gcgcagacat ttacgatatt tacggtcagg ttattgaagg
     4321 tcaggggcgt ctggcagctc tgcgtttcgg tggcgatggt gatgagctga aacgtggtgg
     4381 taaaccgccg gtcaatcacg tcaatattgt cgcgcagcag gcgctgccgg taacgctcaa
     4441 cgaacagggc gaaggctcgg ttacactgcc gattggcgat tttaacggtg aattgcgcgt
     4501 catggcgcaa gcctggacgg cagatgactt cggtagcaac gaaagcaaag tgatagttgc
     4561 cgcaccggtg attgctgaac tgaacatgcc gcgctttatg gcgagtggcg atacctcgcg
     4621 tctgacgctg gatatcacta atcttaccga taaaccgcaa aaactgaacg ttgccctgac
     4681 cgccagtggt ttgcttgaac tggtcagcga ttcacccgca gccgttgaat tagcgccagg
     4741 tgtgcgtact acgctgttta tcccggtgcg agcattgccg ggttatggcg atggagaaat
     4801 tcaggccacc attagcgggt tagcgttacc gggtgaaacc gttgccgatc agcataagca
     4861 gtggaaaatc ggcgtccgtc cggcgttccc ggcacaaacg gttaattacg gtacggcgtt
     4921 acagcctggt gagacatggg cgattccggc ggatggattg caaaacttct cgcctgttac
     4981 gctggaaggg caattgttgt tgagcggcaa accaccgctg aacatcgcac gttatatcaa
     5041 agagttaaaa gcgtatccgt acggctgtct cgagcaaacc gccagcggcc tgttcccgtc
     5101 actttatacc aacgcagccc aactgcaggc gttgggcatc aaaggcgaca gtgatgagaa
     5161 acgccgtgca tcggtcgata tcggcatttc ccgtttgctg caaatgcaac gtgataacgg
     5221 cggctttgcg ctgtgggata aaaacggtga cgaagagtac tggctgacgg cttacgtgat
     5281 ggatttcctg gtccgcgcag gcgaacaggg ttacagcgtg ccgacagacg ccattaaccg
     5341 gggtaatgag cgtctgctgc gctatttaca agatccgggc atgatgtcga tcccgtacgc
     5401 ggataatctc aaagccagta aattcgccgt acagtcttac gctgcgctgg tgttggcccg
     5461 tcagcaaaag gctccgctgg gtgcgctgcg tgaaatctgg gagcatcgtg cagatgccgc
     5521 ttctggttta ccgctgctgc aacttggcgt tgcgctgaaa accatgggtg atacaacgcg
     5581 tggtgaagaa gcgattgtgt tggcgctgaa aacgcggaga aatagtgatg agcggatatg
     5641 gctgggtgat tacggtagtc cactgcgcga caacgcgtta atgctctcct tgctggaaga
     5701 aaataaactg ctacccgatg agcagtacac tttgctgaac acactttcgc agcaggcgtt
     5761 tggtgaacgc tggctatcga cgcaggaaag taacgcgttg ttcctggctg cccgtacgat
     5821 tcaggattta cccggtaaat ggcaggcgca aacctctttc tcagctgagc cgctgacagg
     5881 cgagaaagcg caaaacagta atctgaatag cgatcaactt gctaccttgc aggtgaccaa
     5941 cagtggcgat cagccgttat ggctgcgtgt ggatgccagc ggttatccgc aatccgcacc
     6001 tttaccggcg aacaatgtgc tgcaaatcga gcgtcatatt cttggtactg atggtaagag
     6061 caaatcgctg gactcgttac gtagcggcga tctggtgctg gtgtggttgc aggtaaaagc
     6121 cagtaacagc gtgccggatg cgttggtcgt ggatctgctg cctgcgggtc tggaactgga
     6181 aaaccagaat ctggcgaacg gtagcgccag cctggagcaa agtggtggcg aagtgcagaa
     6241 cttactgaac cagatgcagc aggcgagcat taagcacatt gagttccgtg acgatcgctt
     6301 tgtggcggcg gttgccgttg atgaatacca accggtaacg ttggtgtatc tggcgcgggc
     6361 ggtgacgccg ggaacgtatc aggtaccgca accgatggtg gaatcaatgt atgttcccca
     6421 atggcgggcg accggcgcgg ctgaagattt gctgattgtc agaccgtaaa tgcctcgcct
     6481 gttaaccaaa cgcggctgct ggataacgct ggcagccacg ccctttctcc tttttcttgc
     6541 agcgtgggga gcagataaaa tctggcctct accgctgcat gaagtcaatc ccgcacgagt
     6601 ggtcgtggcg caggatggta cgccgctctg gcgtttcgcc gatgctgagg gcatctggcg
     6661 ttatccggta acaatcgaag atgtttctcc acgttacctt gaagcgctga tcaattatga
     6721 agatcgctgg ttctggaagc atccgggggt gaatccattc tcggtggcgc gcgcagcatg
     6781 gcaagatctc acttcgggac gggttatttc cggtggcagc acgctcacta tgcaggttgc
     6841 tcgtctgctt gatcctcacc ccaaaacatt tggcggcaaa attcgccagc tctggcgcgc
     6901 gttgcaactg gagtggcatc tgtctaagcg tgaaattctg accttgtatc ttaaccgcgc
     6961 tccgtttggc ggtacgttgc aggggatcgg tgcggcaagt tgggcttatc tcggaaaatc
     7021 gcctacgaat ttaagctatt ccgaggcggc aatgctggcg gttttgccgc aagcgcccag
     7081 ccgtcttcgc ccggatcgct ggccggaacg tgccgaagcc gcgcgtaata aagtgctcga
     7141 acggatggct gcgcaaggag tgtggtcccg tgagcaggta aaagagtcaa gggaagaacc
     7201 catctggctg gccccccgac aaatgccgca actggcaccg ctgttttcgc gcatgatgct
     7261 cggtaaaagc aaaagcgaca aaatcactac tacgttggat gccggtcttc aacgacgtct
     7321 ggaagaactg gcgcaaaact ggaaaggacg tttaccaccg cgcagctcac tggcgatgat
     7381 cgtggttgat cataccgata tgcgtgttcg cggctgggtg ggatcggtag atctcaacga
     7441 tgattcacgc tttggtcatg ttgatatggt caatgcgatc cgatcgccag gatcggtgct
     7501 caaaccgttt gtttatggtc tggcgctgga tgaaggcttg atccacccgg catcactgct
     7561 gcaagacgtc ccccggcgca ctggtgatta tcgaccaggt aactttgata gcggttttca
     7621 tggcccgatc agcatgagcg aggcgctggt gcgctcgctg aatttacctg ctgtgcaggt
     7681 gctggaagcc tatggaccga aacggtttgc ggcaaagtta cgcaatgttg gattgccgtt
     7741 atatttgccc aacggtgcgg cgccgaatct ttcgctcatt ctcggcggcg ctggtgcaaa
     7801 attggaagat atggcggcag cgtataccgc gtttgctcgc cacggcaagg caggcaaatt
     7861 gcgcttacag cctgacgatc cgctgcttga acggccttta atgtcgtcgg gggctgcgtg
     7921 gatcattcgc cggattatgg ctgatgaagc gcaacccttg ccggatggtg ccttgccgcg
     7981 cgtcgcccca ctggcatgga aaacgggcac cagctatggc tatcgtgacg cctgggcgat
     8041 tggggttaat gctcgctatg tcattgggat ctggactggc agaccggatg gcacgccggt
     8101 tgttggtcag tttggctttg ccagtgctgt accgctgttg aatcaggtca ataatatttt
     8161 actgtcgcgc agtgcgaatc tgccggaaga cccgcgcccg aactcagtca ctcgtggcgt
     8221 tatctgctgg ccgggcggac agtctttgcc ggaaggtgac ggtaactgtc gccgccgcct
     8281 ggcaacctgg ctgctggacg ggagtcagcc gccaactcta ttactgccgg agcaggaagg
     8341 cattaatggc attcgtttcc ccatctggct ggatgaaaat ggcaaacgtg ttgccgctga
     8401 ttgcccgcaa gcgcgacaag aaatgataaa tgtctggccg ctaccgctgg aaccctggtt
     8461 gcccgcatca gaacgtcgcg ctgtgcgctt accaccggcc tcgacaattt gcccgccata
     8521 cggtcacgat gcccagctcc cgctgcagct gacaggcgtt cgcgatggcg agattattaa
     8581 acgtttaccg ggcgcagcgg aagcgacttt gccgttgcaa tcaagtggag gggcaggtga
     8641 acgctggtgg tttctgaacg gcgaaccgtt aactgaacgt gggcgcaacg tgaccctgca
     8701 tttgacggat aaaggcgatt atcaattgct ggtgatggat gacgtggggc aaatcgcgac
     8761 agtacatttt tcatttcagt
//
LOCUS       DAPGRN010000161         8629 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPGRN010000161 DAPGRN010000000
VERSION     DAPGRN010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.163"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            349..558
                     /locus_tag="RHW33_004233"
     CDS             349..558
                     /locus_tag="RHW33_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HDV1647680.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            669..1520
                     /locus_tag="RHW33_004234"
     CDS             669..1520
                     /locus_tag="RHW33_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647681.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDAASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     SNPESAQ"
     gene            complement(1546..3030)
                     /locus_tag="RHW33_004235"
     CDS             complement(1546..3030)
                     /locus_tag="RHW33_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV1647682.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDEIIES
                     TQQDGSWTSVTSGHGTGKSDMTSIIAILFIMFFPGARVILVANKRQQVLDGIFKYIKS
                     NWATAVSRFPWLSKYFILTETSFFEVTGKGVWTILIKSCRPGNEEALAGEHADHLLYI
                     IDEASGVSDKAFSVITGALTGKDNRILLLSQPTRPSGYFYDSHHRLAIRPGNPDGLFT
                     AIILNSEESPLVDAKFIRAKLAEYGGRDNPMYMIKVRGEFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWVACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKSTADLMYERYGITVQRIRWGKKMHSREDKS
                     LYFDMRAFANIQAAEAVKSGRMRLDKGAATIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLANYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            complement(3030..4223)
                     /locus_tag="RHW33_004236"
     CDS             complement(3030..4223)
                     /locus_tag="RHW33_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDV1647683.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNVQHAGDHIAAEIAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVINRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEENQTASGAPSLEDLDKIARERAA
                     SRRADAALWIEHRREEIADIVDTGGYGDVDAEGISNEAWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(4309..4761)
                     /locus_tag="RHW33_004237"
     CDS             complement(4309..4761)
                     /locus_tag="RHW33_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HDV1647684.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            complement(4850..5893)
                     /locus_tag="RHW33_004238"
     CDS             complement(4850..5893)
                     /locus_tag="RHW33_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV1647685.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMEDGLVVTEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSNSH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKATPQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQRLFFDYAL
                     GVGVITSHSSSVR"
     gene            complement(5921..6100)
                     /locus_tag="RHW33_004239"
     CDS             complement(5921..6100)
                     /locus_tag="RHW33_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HDV1647686.1"
                     /translation="MARKYNKLSREALKMLLDGVSRRKVKQYLVGKQIGVRTAIAVLC
                     RQEMVALKQRMPGSR"
     gene            complement(6105..6485)
                     /locus_tag="RHW33_004240"
     CDS             complement(6105..6485)
                     /locus_tag="RHW33_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDV1647687.1"
                     /translation="MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            complement(6485..6706)
                     /locus_tag="RHW33_004241"
     CDS             complement(6485..6706)
                     /locus_tag="RHW33_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDV1647688.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            complement(6779..7168)
                     /locus_tag="RHW33_004242"
     CDS             complement(6779..7168)
                     /locus_tag="RHW33_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HDV1647689.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            7343..7927
                     /locus_tag="RHW33_004243"
     CDS             7343..7927
                     /locus_tag="RHW33_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV1647690.1"
                     /translation="MPNRIPLDPVLPKNFDCTPNEKRSKAQLDAWWDHPYGVTQPDGK
                     IVVYCLNGGAWDRPSVLCLADNYDEACELAERQQASWVKTRSEPTFMFSKEPPFILAR
                     MPQRPDHQQEIVAEFSSRDEMNLFSLKQEERDRVEVSPTLDHNRMNLAQLAWYSKELE
                     MSIARLENEKAAIQVQHEVVLNRIREMQNDNRGF"
     gene            7928..8284
                     /locus_tag="RHW33_004244"
     CDS             7928..8284
                     /locus_tag="RHW33_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647691.1"
                     /translation="MAKIEYHRDRGNYLEIYDHESLNDINDALYEYCEKTSITDAPDA
                     FVEMPVYLRDIYAIRTPPVSVIHIGYVRLSIEEDEDRYIVRHYTLDRKELPNEWNMSN
                     FYNGEYGLKSANNLRS"
BASE COUNT         2307 a         2205 c         1880 g         2237 t
ORIGIN      
        1 ataaaaatca agaattatta gagcaataaa ttttgagaga aaaatcccac tccaccagcc
       61 aaaaactgga ttgtttttca tagttgtttg acaattgctc taataaatta tagttttgcc
      121 gccgtttcgt aatacaactt tggattcact atttaatgtg tcttcagcgt tgtagagcgg
      181 ctcagaagga aatgagcaaa cagggaaacc ttatacaacg gcattacagc tatgcattgc
      241 tcatcttaca cacagcgcaa tgttgtagga ttaccccagc atggatcatg ggtgaaacag
      301 taggtcagag cttcaggctc tgtgttgtca atacagtgag gcataattat ggctttcatt
      361 ccaccaacca tcgacgacgt tagacattgc tctaacgctt tatctgtaga ccccgccgaa
      421 accgacgctg cccgcgccat tgctgaacac tactcaaaga tatccaatca ggagtaccgc
      481 atcacccaag acgacctgga tgatctcact gacacaatcg aatatctcat ggccactaac
      541 cagccagact cacaataaat gcactaataa atctattatt ttcgttagat ccttctataa
      601 tggtggccaa caactcccag tgtaatccgc tgtgagttgt tggccatgtc aattctggag
      661 gaggatcaat gataaattat gtctacggcg aacaactgta ccaggagttc gtcagcttca
      721 gggatctctt tctaaaaaaa gctgttgcac gcgcccaaca cgttgatgcc gccagcgacg
      781 gtcgtcctgt tcgcccggtt gtcgttctgc cgttcaaaga aacggacagc attcaggctg
      841 aaattgataa atggacttta atggcgcggg aactggaaca gtacccagac ctcaatatcc
      901 caaagactat tttatatcca gtgcctaaca tccttcgcgg tgtgcgtaag gttacaactt
      961 atcagacaga agctgtgaac agcgtcaaca tgaccgctgg ccgcattatt catctgattg
     1021 ataaggacat tcgcatccag aaaagcgcgg ggatcaatga gcacagtgcg aaatacatag
     1081 agaacctgga agcaacaaaa gagctaatga agcagtaccc ggaggatgaa aaattccgta
     1141 tgcgtgtaca cggctttagc gaaacaatgt tgcgcgtcca ttacatttcc agtagcccta
     1201 actacaatga tggtaaatca gttagttacc atgtgccgct gtgtggtgtg tttatctgcg
     1261 atgaaactct ccgtgatgga atcatcatca acggtgaatt cgagaaagca aaatttagcc
     1321 tttatgactc tatagaaccg atcatctgcg accgctggcc gcaggcaaaa atatatcgcc
     1381 tggcagatat tgaaaatgta aaaaaacaaa ttgccatcac tcgcgaagag aaaaaggtca
     1441 aatcagccgc atcagttacg cgcagccgta aaactaagaa ggggcagcca gtaaacagca
     1501 accccgaaag cgcgcaatag ttttccatcc ggcatggtca aattgttatt cattaagcca
     1561 tgccagagct tcatcaacct gcgcttcgtc ttcgacgcta agcacttcat cttggggaac
     1621 atagttcgcc aacatagcga aacaatatgt atcccaatgg tccggtgagt gcaggttgag
     1681 ttttttcttc atatcttcct ttgacatcac cttccattga cctgcggaat ttatccctac
     1741 cggtatcttt gatgcttcct ctatagtcgc agccccctta tcaagccgca tacgccctga
     1801 ttttacagct tctgccgcct gaatattcgc gaaagcgcgc atatcgaaat aaaggctttt
     1861 atcttcacgg ctgtgcatct ttttacccca gcggatacgc tggacggtaa tgccatagcg
     1921 ttcgtacatt agatcagccg tcgatttccc caagccatcg ccatcaatag ctatggttat
     1981 gttcgggaac cgttctgggt tacattctgc gaaaatcttg gcggctaact gcgtttctgt
     2041 aacgtctgtg tattccagca tacgatagtt gattacacgg cgtttatttc gctggccgga
     2101 caccatcatg atattaataa cggacttatc tcgtcctgtg ccaccagcaa cgtcaacaca
     2161 tgcaacccag ccccatcctt tggcaatctt gacctttcgc cgcgtcgccc gctcaacctc
     2221 atcacgacca agaagaaagc catcttgaga tttgggaaat tcaccacgta ctttgatcat
     2281 gtacatgggg ttatcacgac cgccatactc cgcaagtttt gctcgtataa attttgcatc
     2341 tacaagcgga gattcttcac tattcagtat tatcgcagta aacaatccat caggatttcc
     2401 cgggcgaata gctagtctgt ggtgtgaatc gtagaaatag cctgaaggtc gcgtaggctg
     2461 ggaaagaagc agaatacggt tatccttacc ggtcagcgca cctgttatca cactgaatgc
     2521 tttatcactc acacccgacg cttcgtcgat gatatacaag agatgatcgg cgtgttcacc
     2581 agccaacgcc tcctcatttc cggggcgaca ggactttatc aatattgtcc aaacaccctt
     2641 gccagtcacc tcaaaaaaag acgtttctgt aagaatgaaa tacttcgaca accacgggaa
     2701 tctgctaaca gcagtagccc aattgctctt tatgtatttg aaaataccat caaggacttg
     2761 ctgtcttttg ttagcgacca gaatgacgcg agcgccgggg aaaaacatga tgaagagtat
     2821 tgcaatgata ctcgtcatat ccgatttacc agtaccatgg ccggaggtca cacttgtcca
     2881 actgccgtcc tgctgcgtgg actcaatgat ctcatcctgc tgccaggttg gtgtcttccc
     2941 aaacaacaca tcagcggccg caatccagtc ataacgatat agcgccacaa gctcgcgcca
     3001 acgtgggtcc gttacgcaac ttctggccat taatcatcat ccccgtacag tttgcgggta
     3061 acttcttcgt cttcctcctc gtcttcgtcc agatcctgtt caagccatgc ttcgtttgat
     3121 atgccttccg catcgacatc accataacca cctgtatcga cgatatcggc aatttcttct
     3181 ctacgatgct caatccacaa tgccgcatca gcgcggcggc tggcggcccg ttctcgcgcg
     3241 attttatcca gatcttcaag tgatggagcg ccagatgctg tttggttttc ctcatcatcg
     3301 gtattggtct taggagcacg cagatcggct ttgatttgct ccagcatcag gggcggcact
     3361 tttcctccat gcgcctcgat gaattcagcc gcttccagca ctgaccagtt attttcacgc
     3421 tttcgttcgt atgccagctt aacaatgcca gcttgcccca tagacaaagc gtgcttttcc
     3481 gcctcccggc tttcttttcg atagttattc cggatgctgt aaatggtgtt gatcagactg
     3541 cttatctgcg cggaacagct gtttagcatg ctcgcgatac ggtattcagg cggagtacct
     3601 tcatcatcgt ctttttgctg atcgcgcatt tcctgcacca ggcgaataca cgtatccctg
     3661 gcgttctcca gcataaggag atgagaaaga gacttttcaa gaagagtggt ttccagaaca
     3721 tcggccccgg accgacgcaa catagcgcgc gcggccttcc gcgcttcaac gttatctatc
     3781 aggtaatcgc cagcttcgaa ttcaaagcgt tcaccatcat catccagggt gtcgcgttcc
     3841 aggcgatcac gtaaggtccg gtgggcgcgg ttgatcacgt catgatcatc cgaacgatca
     3901 tttatgcgct tattttggcg cttcgcattc tcgactgcgg cactgacaac ggcattaact
     3961 ctctgttttt ccgctatttc agccgcaata tgatcacctg catgttgaac attagagtga
     4021 tcaatgatca tgctttttag tggcttcctg actggcttat ttggcttgcg gctgtccgca
     4081 gtcctggtgt cttctttgaa ggcacggaga taacgacgtg cggtattagg gttaagatta
     4141 aactcggcgg catactgtgc gatggtgtaa ccaccatctc gcgccaggcg agcaaaattc
     4201 ttcttgtgat cgtcccaggt cacttatgct tcctttcgta aaaactcttt ttgacgcgag
     4261 ggtaacgaaa gtcacatgtc aaaaggcccg gaacgggcaa gtaatcaatc agatacgtgc
     4321 ggatgtggca ttaccgtaat gacggtgctg acgggccacc ttattgaaaa gttgacgcgc
     4381 cattacccaa ggctggtgct cccggcgttc cttttcgtcc tgcgtcatat agagttcatt
     4441 ctggagtttt tcatcaaacc ggcgcggagc gcggctacgg cgaaagaatt caggattcag
     4501 agagtggatc tgaaatctac gtgggcgtgt actgtcatca atcaaaacag acgaatactt
     4561 agacacagcg atagccttta agcgcagata aacatcgcgc ttatcgacat ccagatgcgg
     4621 gtattccttt tcaagaattg ctgcgagttc tttcgctgat agaagagatt tagtgcggat
     4681 catgtaatcc gcaatctcgt acgatgttat tcgtgagtga tttatttcca tgaagtggcg
     4741 tccctgccag ttaagtaaca tcctgtcacc tactgattag cccatgtcaa ctaatcaacg
     4801 tggaatataa taccctcgac taaagaaata gcaatacatt agagcaattt tatctaacac
     4861 tcgacgaatg acttgtgata acgccaactc caagcgcgta atcaaagaac aatcgttgat
     4921 gcatcgccaa cctaccgtgc gtcttctccc aattatcgcg gtcacgctca atatcacgct
     4981 ggcatgactg gcacagagga attgcgtaaa tgtcatgcgc gcataatcga ctatgacgaa
     5041 cgatataagg cgtaatgtga gcgccagctc ccgcagctcc acacccacag catggacggg
     5101 aagcaacaaa gtccatgtac tcaggtaatt ttagcgattg cagttttggt attttgaaat
     5161 gcgccatgcc agggtcggag tcaacatcca cagggcatac ttttgcacgc atcggcgcgg
     5221 cgcgttcttc catcatctga acatatgctg tagcgcgatc atcatacggg cgaatatccg
     5281 cctctttcag aggtccgcta tcctgcggag tagccttcat cttatttatt gatatgcggc
     5341 agacttcttc cggcatcagg tgcatcatgt tgcgcatgaa agcccaccag cacagctcct
     5401 gaatacttaa atcatggcta tttgaaaggc ccatttcctg acgggcgaca tccagtatcc
     5461 agttaacgcg attattgtgc agcgtttctt tcagctcatt aaaaccacgc atccgataat
     5521 ggttatcgtg atgccagcac aacaacaccg cgctattgtc tcgttcagcg tggacaatat
     5581 ggttgtcaca ccaactacga tctgcggcct ggcattgacc ctctttccta cgcaaccacg
     5641 ccaccagcgc gtcaattcca ccaatacggc gaaacagttc atcgctgtta aaaaacggct
     5701 gcaacgcctc atttgttgcc atggtttgct cggtaacaac gaggccgtct tccatgtgct
     5761 cgattaactc acgcggcacc ggctccataa taaatttacg gccagcctcc accagctttc
     5821 tgacctcctg atccactttg aatgtggcga cgccaagctc tttctgtaca aagggagtaa
     5881 ttacggcttt cacatcacac ctttcatcac tgattgggct ttatctgctg cccggcattc
     5941 tctgtttaag cgcaaccatt tcctgacggc ataacacagc aatagcggtc ctgactccaa
     6001 tttgcttacc aaccaggtat tgctttacct tgcggcgact cacgccatca agaagcatct
     6061 ttaacgcttc acgggacaat ttgttgtatt tgcgtgccat taatctactc cgcagaacca
     6121 tacaatctac gtaacgtgtc ggcgacagaa gatacagata tctcgccagt cgcagcgcct
     6181 acagtaaggt ctgccagttc aggtgaatca aatacctgca ccccgttacg gcgtagaaat
     6241 aacagcgcac tgtttagcgc ggtacgctta ttggcatcat tgaatatatg ccctctcgct
     6301 gtagccacca ggtaggtggc ggagacttcg aaaaggtcgg tgatctcttc gtaggcaact
     6361 ctggcctgaa ctctcccgat aatggcctct gccctacccg gatctgacat tcccggcagg
     6421 ccgccgtagc ggtttatatt cgcatcatga agcgcaataa gttcttccgg tgatatatgc
     6481 ctcattatcg gttaaccagt tccttgttgg tggagtccag ggtgtcaaac agggatgcaa
     6541 attcagcatc cagcgccgct tttttgtagg cttcgaaagt agccttgctg acaattactg
     6601 ctggctcacg gcctctgcgg gtgatttcaa cctcttcccc ggcttcaaca ttgttgagca
     6661 cttcagaaag gttgccgcgc gcggtacgga agttaatgga ttgcataaac acctcgtgta
     6721 ctcgttatgt gtacacaatt ataaacttca caggcataaa gcaccagcac tttgcagctt
     6781 aaatgaccgg acaatcatca aactccccac tccgggcatc attgatgaca tgagtgatca
     6841 caccaaaaac agcattacta cccgtgtaac catcgtcatc tactggtaac gcctctttct
     6901 tcccggtgct taaatcctcc aggtgctggc gcggatactt tcggtatctc tttatgcgat
     6961 attcaccctc catagcgcac acaagcagag aaccatcaac cggagtaagc gaggaatcaa
     7021 ccaccagcaa agcaccctgc aatattccct cacggtgatg gctatcagct gcccgcatga
     7081 agtaggttgc tgatggatgc ctgattagtt gctgatcaag agaaattcgg ctttcaacat
     7141 aatccgccgc aggagaaggg aagcccatag cgtttttacc tcaataatac tgttcattta
     7201 tacagtatac attaaagaga cacctttggt gcaaacgcgt tacgtacatc aaccaccgct
     7261 gatgattttg tgctctttgc tactattcat caccgcggga tcagcgtaac ctcgttgcca
     7321 atcagttaat aaggaattag ctatgcctaa tcgcattcct ctcgatcctg tattgcccaa
     7381 aaattttgac tgcactccta acgagaaacg ctctaaagct cagctggacg cctggtggga
     7441 ccatccatat ggggttacac aacctgacgg gaaaattgta gtttattgtc tgaatggtgg
     7501 ggcgtgggac cgtccatccg tgctttgttt ggcagataac tatgatgaag cctgtgaact
     7561 tgccgaaaga cagcaggcaa gctgggtaaa aacacggtct gaaccgacat tcatgttttc
     7621 aaaagaaccg ccatttatac tggcgaggat gccgcagcga ccggatcatc aacaagaaat
     7681 tgttgctgaa ttttcctcaa gggatgagat gaatctcttc tcattaaagc aggaagaaag
     7741 ggatcgcgtc gaagtgtctc caactctcga ccacaaccgg atgaacctag cccagctcgc
     7801 ctggtacagc aaagaattag agatgtctat tgcccggctt gaaaacgaaa aagccgctat
     7861 ccaagtccag cacgaagtag ttctgaaccg gattagagaa atgcaaaacg ataacagggg
     7921 attttgaatg gctaaaatcg agtaccatcg cgatcgcggt aattacctgg aaatatacga
     7981 tcatgaatct cttaacgata tcaacgatgc gttatatgaa tactgtgaaa aaacgagcat
     8041 cacagatgca cctgatgcat ttgtcgagat gccggtatat ctccgcgaca tctatgcaat
     8101 acgaacaccg cccgtatcgg tgattcacat tggctatgtc cgcctgtcca tcgaagaaga
     8161 tgaagatcgt tatatcgtgc gccactatac attggacaga aaagaacttc ctaatgaatg
     8221 gaacatgagt aatttctaca acggtgaata tggcttaaaa tccgctaata atttacggtc
     8281 ataatctata caggcatgta taacaacaac gagcctatta gctgtcaaca acgttatttc
     8341 ctcaaataag aaaaagcaga aaaaaaaatt gtttaaggtc acaaattgtg gccttgatgg
     8401 aggagggagg cttattggct atggctaaaa atcaaattag acacttcatg ttgctgtagc
     8461 tcatcaagat ccgaggccac aaaacctttt ccgaaaaact tcacaagtaa ctcactagcc
     8521 gcatcattgt cacctattac acgaaagtca tacggcaacg tcgcaagttg acgatgtaat
     8581 cctgctgaga aagtcgaatc taacagcgcc cagaattccc accgcagga
//
LOCUS       DAPGRN010000162         8560 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPGRN010000162 DAPGRN010000000
VERSION     DAPGRN010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.164"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..963)
                     /locus_tag="RHW33_004245"
     CDS             complement(<1..963)
                     /locus_tag="RHW33_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV1647692.1"
                     /translation="MPIKNNRKILMCVVVMFFFEVLSIPVAIGVTEDTAASNKMTYIR
                     KDADKGDATAQFKLAELYRSGSEGIQSYKDAVYWYSLAAKQGLTEAQYNLGVMYAHGY
                     GVSQNYEKAFECYQMAAEKGFPEAQGNLGVLYSKGSGVIRDYGKAIAWLNKAANSGLV
                     TAQTNLALLYDETNKKDLADKWYRIAAEQGDKVAQYHLGRLYYEGVIIKKDYNKAAFW
                     YDKSASQGFIKAQSALSIFYYEGLGGITVNKYKAIELLRKSATHGDPVAQHNLALIYS
                     VGTKELPLDNKKAYAWFSAAYAHGLDKSVQYKLKMENRLTPEEFE"
     gene            complement(947..5335)
                     /locus_tag="RHW33_004246"
     CDS             complement(947..5335)
                     /locus_tag="RHW33_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1647693.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIHERELILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVLKQHKDHPLARLWR
                     ALPEAVRLSPHTYMMAVSTTGQWLVLSWPERVPEADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRESSLSSPASPRSV
                     SSSQVFPDTLPTGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGQR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIEDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATISPDGKCSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTISH
                     TSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAWNTSGQLRS
                     RHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRHDEYGRLAEKTDRIPEGVIRMHD
                     ERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEV
                     LQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQLEAEYIPE
                     RKLHLYHCDHRGLPQALISPEGETAWQGEYDEWGNLLGETSAQHLQQPYRLPGQQYDE
                     ESGLYYNRHRYYDPLQGRYITQDPIGLEGGLNPYQYPLNPIEHIDPLGLANVTPQMIF
                     GTNYYNTMQGANVGFAPNLSDEEKFKALDDFSSMRGVYNPLDDYVKGWALLPPVIAGV
                     VFTGPVASAAALEGGTLTWGEIAIGSLSSCGAVATTDYMSDHKMPDKSELALCALGGS
                     WGAGKTFVEKIALKMGLSFLSKGGDFKSGVYAAADTGAEELLGATLGKLPFVPENAFG
                     DYITDMTSSEAVDKFKGDDKKDADKK"
     gene            complement(5411..7552)
                     /locus_tag="RHW33_004247"
     CDS             complement(5411..7552)
                     /locus_tag="RHW33_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV1647694.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQKLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRQIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(7762..8280)
                     /locus_tag="RHW33_004248"
     CDS             complement(7762..8280)
                     /locus_tag="RHW33_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV1647695.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT         1725 a         2655 c         2055 g         2125 t
ORIGIN      
        1 ttcaaactct tcaggagtta atctattttc catcttcaat ttatattgaa cacttttatc
       61 aagtccatga gcgtatgcgg cactgaacca ggcatatgcc tttttattat cgagtggtaa
      121 ctctttcgtg cctacggaat agattaatgc aagattatgt tgtgcaacag gatcaccatg
      181 tgtggctgac tttcttaaca gttctattgc tttatattta tttacagtaa tcccacccag
      241 tccttcatag taaaagatac ttagtgctga ttgtgctttt atgaaacctt gacttgccga
      301 tttatcatac caaaaggcgg ctttattata atccttttta ataataacgc cttcataata
      361 gagtcgccct aaatgatact gagcaacctt atccccttgc tccgcagcga tacgatacca
      421 cttatctgcc agatcttttt tattagtctc atcatatagt aaagcaaggt tagtttgagc
      481 cgtaactaat ccagaatttg cagctttatt taaccacgct atggccttac cataatctct
      541 tatcacgcca ctccctttac tatataatac tcctaagttt ccctgcgctt ctggaaaacc
      601 tttttcagct gccatttgat aacattcaaa tgctttttca taattttgag acaccccata
      661 accatgagca tacataacac cgaggttata ctgagcctct gtcagcccct gttttgctgc
      721 aagcgagtac caatatactg catctttata actctggatt ccttcagaac cacttcgata
      781 taactccgcg agtttaaatt gcgcagtagc atctccctta tcagcatctt ttcttatata
      841 tgtcatttta ttggaggctg ctgtgtcttc tgtcacccca attgcaacag gaatactcaa
      901 cacttcaaaa aaaaacatca caacaacaca cattaatatt tttcgattat tttttatcgg
      961 catctttttt atcatcacct ttaaatttat ctaccgcttc actagaagtc atgtcagtaa
     1021 tataatctcc aaatgcattt tcaggcacaa agggcaattt acccaaagtt gcacctaata
     1081 attcctccgc tcctgtatca gcagcagcat aaacaccaga tttaaaatcc cctcccttac
     1141 ttaaaaatga caatcccatt ttcaaagcta ttttctcaac aaacgttttt cccgctcccc
     1201 aagaaccacc taaagcacat agcgccaact cggatttatc cggcatctta tgatcagaca
     1261 tatagtcagt cgtagcaacc gcaccacatg aactaagact tccaattgct atctcccccc
     1321 atgtaagagt ccccccttct aatgcagccg cacttgctac tggcccggta aatactactc
     1381 cggcaataac aggaggcagt aaagcccatc cttttacata atcgtctaat ggattataca
     1441 ctccacgcat acttgaaaaa tcatccaacg ctttaaattt ttcttcatct gacaggttcg
     1501 gggcgaatcc aacattagcc ccctgcatag tgttataata attagttcca aatatcattt
     1561 gcggcgttac attagctaac cccaacggat ctatatgttc aataggattg agtgggtact
     1621 ggtaaggatt taatcctcct tccagcccaa tcgggtcctg ggtgatatac cgtccctgca
     1681 acgggtcata gtaccggtga cggttgtaat acagccccga ctcctcatca tactgctgtc
     1741 ccggcagacg gtacggctgt tgcaggtgct gcgcgctggt ttcgcccagc aggtttcccc
     1801 actcgtcata ctccccctgc cacgccgttt ccccttccgg gctgatgagc gcctgcggca
     1861 gtccccggtg gtcgcagtgg taaagatgaa gtttccgctc cgggatgtat tccgcctcca
     1921 gctgcgcggc catctgctcc gccgtcagcc cgcactgcgc aagccatgcc tcactctccg
     1981 cactcaccgc tcccgcgcgc agttcccgct ccagcctccc cagcatcacc gacagctccg
     2041 ccggtagcgt cacacccgtg tcctcttgca acacctccgc caggctccgg tgccgcgcct
     2101 tcgcctgttc gccattctct gtttcgattc tcaggagcgg cgtgaagctc cccggctggt
     2161 ataccgtctg gatacgcgtg gtgccggtct gtatcgtcgt cagcctgtcg ccgtcccacc
     2221 cgtaccatgt cacctccggt ttacgcgaca acgacatcca ccccgtcagg tcacgctccc
     2281 gccgccatac ccgttttccc gtccggcggc ccagcgggtc atagaggtaa cggctctcca
     2341 cctgtggttc gccgtgctgt atccgcgtgt agaacaccag gcggtgctgg ctgtcgtagt
     2401 ggtagtggtg ggtccgctcg tcgtgcatcc ggataacccc ttccgggatg cggtccgtct
     2461 tctccgccag cctgccgtat tcatcgtggc ggtagacata gtgcgcatct tccgcgatgc
     2521 ggttatccgg ccacgccgtg agcgtgctgt ccgggtgcag ttccgggtcc ggcagccggt
     2581 ttcccgccgg gtctgttgca tacgggatat cgatatccag gttcgccgcc gtggtgtgga
     2641 cgcccgtcag ccttcccgtg tcactgtaac ggtactcccg gctctcctgc gggccgctga
     2701 tgcggattag ctgtccgttg tcgttccagt cgtagtcacg gtcaagctgc gggaggttca
     2761 ggtgcctgct ccggagctgg ccgctggtat tccaggcggt ggcaagttca tatgcctccc
     2821 cgccgaagct gcgggccgtc tcacggtgca gccggtcccg cgtgtactcg accagtggcg
     2881 ttccgcccag cttcatcccc gcaagataac cgctgccata cgtcagccac tctaccggcg
     2941 gcagaccgtc cggctcctga cgggtcgcca gtccctgttc gctgtacgcg tgccccgtct
     3001 catgctccca cagcatctcc cccgtctccg ggttctccac cgtctgccgt tcgcccgtca
     3061 ggcggccttt atcgtcatag ccgtagtgga ccgccacacg gtggccttca ctggtatggc
     3121 tgatggtggt taaccacccg tgctcatcat actgccactg ctccgccggg tcgccgttca
     3181 ccgtccggtg cgtgatgcgg tccgacgcat cgtaatgcca gagggtgata agtccctcat
     3241 cttcactctg cgtgagtttt ccggtcaggt catagtggta acgttgcgtc cgcccgtcaa
     3301 aaccgcgctg ttcagtcagc ctgtccaccg ggtcataccg gaacgtggac tggctgccgt
     3361 tctcgttggt cagcacggtg atgcgcccgg cagcgtcata ccccatgctg cgcgtaagac
     3421 cgccttccgt cacactcacc ggacgcccgc gtttatcata ttcgatggtg ctgcatttac
     3481 cgtccggtga tatcgttgct gtcaggtcgc ctgcggcgct gtactcataa cggatttcac
     3541 gcccctgcgc atccttctga ctgaccagtt gcccacgcgg gttataactg ctgtaagtgc
     3601 tgatgccttc ttcccggtga acggcgattt gttgaccgta ccggtcgtac tcataccgcg
     3661 tcgtgtaccc cgagcagtcc gtaaaggcca gcagttgacc gtaacggctc catgccatct
     3721 gttttgtact gcccgtcgca tcctctatcc ctgtcggcag ctcactcgcc gggtcatcat
     3781 agctgtaact cgtcgtctca ccgctgcgtg aggtctccgc cgccagcctt cccttctcat
     3841 catactcccg gctgctgcga agcccgtccg ggtacgtcac cgacgtcacc tgccgctggc
     3901 tgttatagcc ataccgtacc gtcctgccgt ccggccccgt caccgccgtc accgcacccg
     3961 acgccatatg caggctgtac tccgtccgct gccccgccgc atccgtctgt gccttcagcc
     4021 tccccgcctc atcatactcg ctgcgggtga tgctcccgtc cgcatgctcc ttcttcacca
     4081 cccgcttcag gccgccttcg ccctccgtgt acagcacctc ccgccggttc aggctgtccg
     4141 tgatggtcac acggtcctgc ccgtactcaa agcggtagtc cagcccctcc gggttgacct
     4201 gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc cctgcataat
     4261 ggtgcgccac catccgcccc gcgtgctccg catcataagt aaacccgcgc acctgcgtcc
     4321 cgctgcggtc atacaccgcc cgcagttctc cgctggccgt gtacgtgtag cgcgccagcg
     4381 gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccacacc gcctccagcc
     4441 ggataccgtt atcggcaccg tactctgtac cggtgggcag cgtgtcgggg aaaacctgag
     4501 aagaggaaac agaacgggga ctggccgggg aagataagga agactcgcgc tgtttacgga
     4561 aggcttccgc ccgctgcgcc tgtgtggtca gcgccaggtg aaaacggcgg cccgcgccgt
     4621 ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgccctgtga aaggccagcg
     4681 tccgaccaaa gccatccacc acgcccgtca gcacccggta tgcgggcggc tccggcggca
     4741 gcacctcgtc cgcctccggc acccgctccg gccagctgag caccagccac tgcccggttg
     4801 tgctgacagc catcatatag gtgtgtggac tcagacggac cgcttccggc agcgcccgcc
     4861 agagccgggc cagcgggtgg tctttgtgtt gtttcagcac cccacccctt gccagccaga
     4921 gtgactcgct gcggctgtag cttatctcgc cgggaaacag cggctcaaag tgaatgctcc
     4981 tgccgccgtt gtcgttgagt atcagttccc gctcatgaat ctgtaagcgg atatcaaagg
     5041 gggctttcca gccggggcca aacaccccca ccggcgcggg cgtcctggtc cggtaactgc
     5101 tgtaggcacg ggaaagaatg aacggcagcg ggccgggcag cgcaaggtcc gtctcgcccg
     5161 gcagcacctt cgcacccagt agcgggttca ccgggttagc ataggtaatc cctcccggac
     5221 acaccgagca ggccactccc gtcggcgcac ctatcagcac ccccgctgaa ccctgcacaa
     5281 tatccagtcc cttgcgggtc atatcaccct gccgcgccgc cggttttccg cccatgaatc
     5341 aattccttct gcttttaata tggtttatct ggtcactgca ccagttccgt tttgattttt
     5401 tgcatctgac tcaaaattgc cgcatatccg gcattccggg gggtaaatct ttcggcacag
     5461 gcgcatccgg tacaggttgt gtggcaccac agttccagtt aatcaccggc gcatcaccgt
     5521 tcacatccga ctccccttcc agatgaatgt gcgtaccatt aagaaatatg cttccgtcct
     5581 tcgtcagcac cagccttgcc ttaccacagc agagttcaag atgctcaccg gcagaataaa
     5641 ctgctgtttg tccggtgttt tccttcatcg tcttgcccac cgagaaggtg acgttattcc
     5701 ctacattgac gctgtagccc attcccacca tcagtgattt atgcagccct acctgtgagg
     5761 actgcaacaa cgccaccgag gtattcataa tgccgcctac cgtcgtctga tacgccacac
     5821 ccaccgtcag cgcacgaatg atccccacct tttcaacctg gtttgatccc actttgataa
     5881 tctggttact gcctaccgtt tccacctggt tgacaccaac cgtctgtatc tgattaacgc
     5941 caatgttctg gatctggtta ttggtaacgg tgatcgcctg gttgttacct attttttcgg
     6001 catggttgtt tatcacatcc gtggtgcggt tattcagcac ctcggtgttc atgttcttct
     6061 gcgcgtggat gtagacctgt tctttccctg tcgcatcgtc aaacttcagt tcattaaacc
     6121 cgctgccctt ataggttttc gaacgaatgg tcatctgcgt ctttgttccc ggcaggctgc
     6181 cgggtgtgcg gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc
     6241 cgttgaggaa gtccacaatc acctcctgac ccacgcgcgg tatcgcaagg ttaccgaatc
     6301 cggtgcctgc ccacgcctgt gccacacgga tccagcatga actgtcctgg tttgacgggt
     6361 tataacggtc ccagttaaat ttcacccgca cgcggccatg ttcatcacag aagatttcct
     6421 cgcctgccgg tcccgtcacg acggcgctct gcgggccgtc caccagcggt ttcagcaacg
     6481 gctgtggtcg ccatgttctg tctgccggta ttaccgcaaa atggttatcc agcgtggtgc
     6541 ctgaacccct gcgtcccggt accgcctgcg gctgttcgcc gtgaagatcg ctcgccacca
     6601 cctgccattc ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt tgccgtcccg
     6661 gccatatctc cggcgaacgg cttgttccgc gcgccacttc tgcgttgttg cgccagccat
     6721 ccatctgcca gcgggcaaag ttctgcccgt gggcgccctt gaaacgtccg gggtagtcat
     6781 acacttcata ctgtgtgcgc tggtaatcct ggtactggcc ttcctgatca aaacgccctg
     6841 cccagccggg tcgtttaaag gtgtagtctt tggtcaccac ggaagaaggg cggatttgtg
     6901 cgctgtagcg aaactggctg atgcagaggg tgcttacctc ggtacgggtg ttcggattcc
     6961 aggggatctc aaaggactcc ggcagataac gcacggtatc gcacaggacc aggctctggt
     7021 cggtactttt ttgcgcgtgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc
     7081 acaggaaatc gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgtggct
     7141 cgctgaacag cgggctccac tcggtcaccc cgttttcctg caatatcgtg ccgaggatgc
     7201 tttcgatgtc ctcattctgg aagatacgga agttctggcg cagccctgtg cgccacagcg
     7261 gcgggcatac cttcatgctg tacagcttct ggtttttgtc gttctccccc agttcaaacc
     7321 aggtcaccac acctttcacc cggcgctgta cgtcatcgcc ctgccatatc gtcaggtagg
     7381 ccattttgtc gaggatctgc tggaattcaa gggagagaaa ctgctggctg accagagaga
     7441 gatcgaggga aaaaagcgaa gagagtgact ggttcagatg aaaggagacc accgcaaaag
     7501 catccggtgg caggccgtcc acttccagcg tgaaacgtaa tccggttgac atagcaacct
     7561 ccggggttaa ggaaaaacag acagttcact gttaaagcgg ctgtgacagt acagtgttac
     7621 gcttgcgttc aggcacgcta ccgccgaagt ttccggcagc cgccatctcc cgtgaacgta
     7681 accccttact gacaaccttt ctttctacgg cgtaatacaa aaacagcatc cggcctgata
     7741 gccggatgct gttggcttaa cttatgcttc cagcggtgcg cgccagtcgt cggcaccgga
     7801 ggtgccagat ttgacgtgtt cccattcaat tttgcggtag gccagtgaaa cttcgagcaa
     7861 ctgggtgaac tcgcgtttcg ccgggtcctg gcaatgcggc atatgcagtt tcatatcgac
     7921 aatcgtcgaa tcagtcaggc gggtagtgaa ataatgctcc tgtttgcctt ccacagaggt
     7981 acgccaccag tgaagttcag tggttggcag catttcgccg gaggccagtg cgttgtacat
     8041 cagcggaacc gctttgttca gcgccacggt aaaaatgaaa ggcttgtgag cacgctgacc
     8101 cgcaggctga ccggactgag gatcggtcgg gacagtaacg ttatgcagga attcctgcac
     8161 cagcatctcg tcttcgtgcc cctgcacata gatgttgccg acagaatcag cggtaaatgc
     8221 acctgcagta atatttccct gggtctgacc agtgatggag atataacacg gtgttggcat
     8281 ttgtaaactc cttgttaacg tggttaatta ctacttacaa tgacctgccg ttaatcccag
     8341 acaggcagat gcagctcact tcacggaacc gcatcatttt tcagtatttt ttacatacaa
     8401 accgagctag aatttacggt tgtcgtaaaa actattttta aaatcattaa atctcttatt
     8461 cagcaataac ttaataatat atatctggat aaccataccc aactgttcaa agtccgtatt
     8521 gcctccataa aatggccaaa gctgaatcat gaataaatat
//
LOCUS       DAPGRN010000163         8396 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPGRN010000163 DAPGRN010000000
VERSION     DAPGRN010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.165"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="RHW33_004249"
     CDS             complement(494..1066)
                     /gene="hdeD"
                     /locus_tag="RHW33_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV1647696.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1321..1653
                     /gene="hdeA"
                     /locus_tag="RHW33_004250"
     CDS             1321..1653
                     /gene="hdeA"
                     /locus_tag="RHW33_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV1647697.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            1769..2095
                     /gene="hdeB"
                     /locus_tag="RHW33_004251"
     CDS             1769..2095
                     /gene="hdeB"
                     /locus_tag="RHW33_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV1647698.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2159..2806
                     /locus_tag="RHW33_004252"
     CDS             2159..2806
                     /locus_tag="RHW33_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV1647699.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(2858..3628)
                     /locus_tag="RHW33_004253"
     CDS             complement(2858..3628)
                     /locus_tag="RHW33_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HDV1647700.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLHEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCILHDLNLAARYADRVVLMQKGKVIA
                     NGKPQGVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(3625..4581)
                     /gene="chuU"
                     /locus_tag="RHW33_004254"
     CDS             complement(3625..4581)
                     /gene="chuU"
                     /locus_tag="RHW33_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ChuU"
                     /protein_id="HDV1647701.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMMFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(4668..5291)
                     /gene="chuY"
                     /locus_tag="RHW33_004255"
     CDS             complement(4668..5291)
                     /gene="chuY"
                     /locus_tag="RHW33_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HDV1647702.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSDRAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRVQNQECHGFVRRADVAAHIHELANTPALNKQIYSLIEPDLKPA"
     gene            complement(5291..5785)
                     /gene="hutX"
                     /locus_tag="RHW33_004256"
     CDS             complement(5291..5785)
                     /gene="hutX"
                     /locus_tag="RHW33_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDV1647703.1"
                     /translation="MSHVSLQEFLKTEPDGTLEVVAEQYNTTLLEVVRNLPFSTVVPG
                     DKFDTVWDTVCEWGNVTTLVHTADVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHTLAASL
                     KEHA"
     gene            complement(5798..7135)
                     /locus_tag="RHW33_004257"
     CDS             complement(5798..7135)
                     /locus_tag="RHW33_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDV1647704.1"
                     /translation="MNAINTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMISQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSRIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDEAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGVETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIVREKP"
     gene            complement(7155..8069)
                     /gene="shuT"
                     /locus_tag="RHW33_004258"
     CDS             complement(7155..8069)
                     /gene="shuT"
                     /locus_tag="RHW33_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     ShuT"
                     /protein_id="HDV1647705.1"
                     /translation="MPRIITRPFLFSPLTLCISALASAAKSTVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGERVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGDALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAADPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         2254 a         1999 c         2031 g         2112 t
ORIGIN      
        1 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
       61 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca
      121 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
      181 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
      241 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
      301 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
      361 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac
      421 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgagtg
      481 acaaccggga taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
      541 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
      601 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
      661 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
      721 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
      781 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
      841 aattatgact gcggttgctg aataatccaa caataagcgc aatgcccgaa cagattaata
      901 atgcacccac taccgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
      961 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgc tttttcagca
     1021 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     1081 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     1141 cgatttaata aaaatttcct aattacagta tctgatgcat ctgtaactca ttgtattgaa
     1201 ataaaaatat ctgattttga tattttccat caacatgaca tatacagaaa accaggttat
     1261 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     1321 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtgagcaat
     1381 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg tcaactcctg gacctgtgaa
     1441 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     1501 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     1561 ccagctatcg ttcaggcttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     1621 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
     1681 cttgcatatt gaacaggccg gaatatcttc tttaaaagca gctattcctc ctgttcatat
     1741 ataatctcta tattgaatgg gttacaaaat gaatatttca tctctccgta aagcgtttat
     1801 ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca caatctgcgc tggcagccaa
     1861 tgaatccgct aaagatatga cctgccagga atttattgat ctgaatccaa aagcaatgac
     1921 cccggttgca tggtggatgc tgcatgaaga aacagtatat aaaggtggcg ataccgttac
     1981 tttaaatgaa accgatctca ctcaaattcc taaagtgatc gaatactgta agaaaaaccc
     2041 gcagaaaaat ttgtatacct tcaaaaatca agcatctaat gacttgccga attaatgagg
     2101 tacaagtaaa aaggagtagc aagttgagcc atcttgctgc tcctttttgc atttttatat
     2161 gacagcagaa tttattatac gtcttatact cgcggcaatt gcctgtggcg ctattggcat
     2221 ggaaaggcaa atgcgcggca aaggagcagg gttacgcaca catgtattaa ttggcatggg
     2281 aagcgccctg tttatgattg tttcgaaata tggttttgct gacgtgctgt ctttagatca
     2341 cgtcggactg gaccccagcc gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg
     2401 tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt ttgacgacgg cagcggatat
     2461 ctgggtgacc gccgccatag gtatggttat tggcagtggt atgtacgaac tgggtattta
     2521 tggttcagtg atgaccttgc tggtgctgga agtcttccat caattaacct tccgcctgat
     2581 gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga
     2641 ctggtttaag cagcaaaaaa taaaaactga tttagtttcg ttgcaggaaa atgaagatca
     2701 tgaagtggtc gccattgata tccagcttca tgccacaacg tcgatagaag atctgttgcg
     2761 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tatagttatt
     2821 tattcgcagg agtaccagtg cggtactcct gttgctttta atgatcgagg acgatcagtg
     2881 gtgaatggtt ggcaggatct ttaagtacgg taatatccgc gccgtagagc attgtcagcg
     2941 cctgctgtgt caatacgccc tgtggtttac catttgcgat aacctttcct ttttgcatta
     3001 atacaacacg gtctgcataa cgcgcggcaa gatttagatc gtgcaatatg cagcagacat
     3061 tgaactgccg ctcgtgaact aactggcgta ataaacgaaa caagtgctgc tgatggtgaa
     3121 tatccagcgc cgaagttggt tcatcaagaa ataaccattt cggtgaggga gtaggttccc
     3181 agagttgcac cagcaggcgc gctaattgca cgcgctgttg ttcaccaccg gataattgcc
     3241 gatagtcgcg gtttgctaac gcctgacagt cacataacgc cattatttgt gctgtttcat
     3301 tatcctgatt accagtacga tgcggatgcc gccccatctg gataacttct tgtacgctga
     3361 atggaaacgc catgtggcta ttttgacgca tcacggcccg gtgtttcgcc agttcagtaa
     3421 tcgaccattc atgtaatggc ttattaaaca accgacattc gccgctatca ggttgaagat
     3481 agccggttaa ctggcgaagc agcgtagatt ttcccgcacc attagggccg agaatggcaa
     3541 caatttcccc accgggaaaa gtcagcgaga cattatcagt cagacgccgc ccctgaaggc
     3601 tataaaccag attctgggcg ctgatcatgt gatcctccga tttcgcagaa tcaaccacat
     3661 aaaataaggg ccaccaagca ggctggttaa taatcccacg ggcatttctg ctggctgtac
     3721 cagcgtgcgg gcaagcgtgt ctgccattag caataaacag gctccggcga gggcggaaca
     3781 agggatgagc cagcggtgat ttgctccggt agtcatgcga atcagatgcg ggatcaccag
     3841 gccaataaag ccgataatgc cgcttaccga tacggcagca ccaacgagca gtgagctcac
     3901 taacagtaat tgctggcgtt tacgcttaac gttcacgccg aggtaatggg cttcttcatc
     3961 accgagctgc agtaaattca gcgttccggc gagacaagtt gttgcgataa tggccggaag
     4021 gatgaatgaa ctggcaacca ataacgtcgg ccattgcgcc tgtccaagat tgcccatcat
     4081 ccacaatgtg agttgtcgta gctgctgctc atcgccgata tagctcagga tgccgatcgc
     4141 tgcgccgcac agggcattga tggcgatacc ggcgagtaat aattgcatca tgctgccatc
     4201 gcgatggcgc tgcgtgatga gaaaaatgat ggtgcagacc actaaacttc cggcaatggc
     4261 aaacatcatt tgctcataga gcaccagcac cactgggaag gagaagggca gcacaatagc
     4321 aacgccaacc attaatgcag aaccgctact gacgccaagt aatcctgggt cagccattgg
     4381 gttgcgaaaa agcccctgca taattacccc tgcggttgcc aacgctgcac ctaccagcac
     4441 cgccagcaaa acccttggta gacgaatatt gagccagata tcgcggtatt cctcatcaaa
     4501 aagggcatca aaactgatct ttaacgctcc ctgactggca ccgaacagca ccagggcaag
     4561 caataatagt gatagcccca taaagaccct ggcggatgaa aatgaatcct tgagcacgca
     4621 aaactccgtt atgacgttgc ggcgcggaga gtctgcgccg catgggatta cgccggtttc
     4681 aggtcaggtt caatcagact gtagatctgc ttgttgagtg ctggcgtgtt tgccagttca
     4741 tgaatgtgtg cagcgacatc ggcacggcgg acaaaaccat gacactcctg attttgtacc
     4801 cgttgtgctt tgcctgttgc tgcgccgtca agcagaccac cagggcggag aatggcgtag
     4861 tctagctggc tggtttgcag ccagctttcc gccagtgtct tttcgcgcac cgcctgacca
     4921 aacgcggctt ttgcccgatc cgataaaaac ggccagctat cgccgcatcc caaagaggtc
     4981 accagaatca tgcgactgat gccggccttt tcggcctcat caatcactgt tctgtgcgcc
     5041 agataatcct gagctcctcc cattgttgag atgataagcg catccgggcc agctgcgcgg
     5101 caagcagccg cgactacgct ggcatcacag gcgtcgcctg taaaaacctg tacgccttgt
     5161 tgcgccagtt tcgtggcggc atcggcatga cgaatgacag caacgaccgg acgttgttcc
     5221 gccagcgcca gttcaagcgt tctggcaccc acaccttttc cgcctgcgcc aaagagtagc
     5281 cacggcgtca tcaggcgtgt tcctttaacg atgccgccag agtatggaag gcgctgactt
     5341 gttcgctcag gagctgacga tggtcatccc tgccgaggaa gattttcagc atggcgctgc
     5401 cttctttgtt gaaaaagaga atggacgcgg tgtccatgcc cataaattta cgttcgatta
     5461 aggcaatatg cgtgcagttt tctgctttga tatgcccgga catgccgtgc tttccgcgta
     5521 ggttgaaata gccgtggcga tggaaacctg aaggcagttc gccgctaaat tcgaggatta
     5581 catcggcagt atgtactaac gtggtgacgt tgccccattc gcagacggta tcccagacag
     5641 tatcgaattt atcgcctggc actacggtag agaagggtag atttctcact acctccagca
     5701 gtgtggtgtt gtattgttcg gctaccacct ccagcgtacc gtccggctcc gttttcaaaa
     5761 attcttgtaa cgagacatgg ctcataaatc actcctgtta tggtttttca cgcacaatcg
     5821 ctggcgcatt aagtacctga attagttcat taagagactg caaaatattg cttgcccaga
     5881 aacgaccttc attagtcagc cgcaggcaag ttgaagcatc gcgacttaag cctttttggt
     5941 gccattgagc cagtaacggc gcgagttttt cggcatgtgg cgtaagttca tccagcggta
     6001 cacgcgctgt ttcaacacct gactgcaagg tatgacgcca ttgcgcatta cgttcggcgt
     6061 tacgcatgat catcatcagc ggttttttgc ctgccgcgac ggattcatgc caggtctgaa
     6121 gattgcgttc gttcatccag gagtaaccat taatcgaacc accggctccg gaaccaaagg
     6181 ccagacaatc ggcaccttgt tttatcagca ggttatagag attgcgttcg cgtgtggtac
     6241 ggccccagtg gctgttacta atacagcgcc aaccggcttc gtccatgaag tcacaccctt
     6301 gcagataaag atcgcgacgt tctgccggag agggcacggt agtgcgccca ttttccacgg
     6361 ctttgcccag cggggtattg ggcaggacat tgagcgcata gagatcgacg ccgtcgagac
     6421 cgatatcgcg agcaatagcc agatcttctc cccaggtttg cgcatcctga cccggcaggc
     6481 caaacagcag gtcacagacc accgcagcac ggtcacgttt gaccaggctt tccataaacg
     6541 caatggcggt tgggccatcc gaggtgcggg ccattttctt gcggattctg ctgttaaacg
     6601 actggatacc aattgagaag cggtttgctc ctgcatcaag gcaagcatcg attcgctcag
     6661 cgtcaaaatt cagtacccgg ccttcgatag tgatttcaca atcaggggcc agtggcagct
     6721 tttctcgtaa tgtggtgatg attctggcca aatcgtgtgc cgaaagcgcg gaaggcgtac
     6781 ctccaccgaa atagaccgcg tggatggggg cagactgatg caatacgctg tccgcttcca
     6841 tttctatttc acgaatcagg gcgtcggtat aatgagcaca cgcatcttca ttaaaacggt
     6901 tctgataaaa accacaaaac gtgcagtgtg tcgcacaaaa aggaatgtgt aggtaaacca
     6961 gtcgcttacg tggtgatacc gtttgactga tcatctcttg ccaggtctgt gccagttgct
     7021 ctttggcaac cggaatggct ccacgaaacg gcatcatggc gcgcctgtct ttaaatggct
     7081 gatcaccgtc taaagcaaaa tgtggcgtga gatccagggt gttaattgcg ttcattcgtc
     7141 tgatttaaat ccatttattg cggccaaagc tgctggtgta aagactcaac gacatcagca
     7201 atacgcggac ccattcccag aatcaggttt tgatcaacgg tgataatgcg ctggtttttc
     7261 catgcggcag tgtgggtaat tccggcaata gaacgtagcc gattaatatc gccatcaacc
     7321 atttgtgagg ttacgacaat cacttctgga tcagccgcaa tcagtgattc tgcactgtag
     7381 cttttgtact gctggtgggt tgcgacgttt tctgctccgg caagcgaaag aatggcgtcc
     7441 gcgacgctgc ctttaccggc aacttgtggc gcacttccgc ctgcggagag aataaacatg
     7501 gctttaaccg gggctttttt ggccgccacg ttttgctgta ctcgctccag gcgttggttg
     7561 atctgtgtca ccagcgcgtc cccttgttca ggtacctgta acgtttttgc cagttgacga
     7621 atgttggcgt acatctgctc aagggtggca ggcacacgcg gcagggtaac gacgttgact
     7681 ttctgcgccc gcagctggtc gagcacaatt tgcggtccag catcctgcca ggtaattacg
     7741 ctatccgggc gaagtgacaa aatgccttcg ctgctgagtt gtttccagta gccaatatgc
     7801 ggcagtttgg cggtttctgg tggataagat gtcgtttcat cgacgccaac cacgcgttcg
     7861 ccagcgccca tcgcgtagat tagctccgtc agcgatcctc ctgcgaccac gatacgttcg
     7921 gcagccgtta cgctaaaagt ccagctaaga gccagtaatg ccgtaaacaa tttttttcgt
     7981 tttaccgtgc tcttcgcggc agaggccagg gctgaaatac ataacgtcag tggggaaaaa
     8041 agaaacggcc tggtgatgat ccttggcata atatgagaac tcttatcaaa ttgagaatga
     8101 tcatgattat catttgagtt atatatgaaa tacaaccgtc gattcaatac gcaaggcgtt
     8161 acaagcgtat ttatttttga tttttatcaa gatgcttcgt cagctaacgt tcttgttacc
     8221 gactaaattt catcgcggga aaaagctgag aacccagaga tatcgaggct tgcagagcaa
     8281 agagaagaaa aaattatcac tgttaacgac aaaatttagt gatgaggcat ggggggataa
     8341 ccataaaaac aaggtggttt acagggcgca ggataggttt cataaccccc tgttta
//
LOCUS       DAPGRN010000164         8197 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPGRN010000164 DAPGRN010000000
VERSION     DAPGRN010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.166"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..542
                     /gene="ftnA"
                     /locus_tag="RHW33_004259"
     CDS             45..542
                     /gene="ftnA"
                     /locus_tag="RHW33_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV1647706.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(580..819)
                     /locus_tag="RHW33_004260"
     CDS             complement(580..819)
                     /locus_tag="RHW33_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV1647707.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            1010..2221
                     /gene="tyrP"
                     /locus_tag="RHW33_004261"
     CDS             1010..2221
                     /gene="tyrP"
                     /locus_tag="RHW33_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV1647708.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2283..2948)
                     /gene="yecA"
                     /locus_tag="RHW33_004262"
     CDS             complement(2283..2948)
                     /gene="yecA"
                     /locus_tag="RHW33_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV1647709.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3144..3230)
                     /locus_tag="RHW33_004263"
     tRNA            complement(3144..3230)
                     /locus_tag="RHW33_004263"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3194..3196),aa:Leu,seq:taa)
     gene            complement(3243..3316)
                     /locus_tag="RHW33_004264"
     tRNA            complement(3243..3316)
                     /locus_tag="RHW33_004264"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3282..3284),aa:Cys,seq:gca)
     gene            complement(3370..3445)
                     /locus_tag="RHW33_004265"
     tRNA            complement(3370..3445)
                     /locus_tag="RHW33_004265"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3410..3412),aa:Gly,seq:gcc)
     gene            complement(3597..4145)
                     /gene="pgsA"
                     /locus_tag="RHW33_004266"
     CDS             complement(3597..4145)
                     /gene="pgsA"
                     /locus_tag="RHW33_004266"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV1647710.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4202..6034)
                     /gene="uvrC"
                     /locus_tag="RHW33_004267"
     CDS             complement(4202..6034)
                     /gene="uvrC"
                     /locus_tag="RHW33_004267"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV1647711.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGIRPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6031..6687)
                     /gene="uvrY"
                     /locus_tag="RHW33_004268"
     CDS             complement(6031..6687)
                     /gene="uvrY"
                     /locus_tag="RHW33_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV1647712.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6983..7159
                     /locus_tag="RHW33_004269"
     CDS             6983..7159
                     /locus_tag="RHW33_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647713.1"
                     /translation="MIKIFIGHYINAFNSTVDGTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7146..7370
                     /locus_tag="RHW33_004270"
     CDS             7146..7370
                     /locus_tag="RHW33_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV1647714.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7438..8160)
                     /gene="sdiA"
                     /locus_tag="RHW33_004271"
     CDS             complement(7438..8160)
                     /gene="sdiA"
                     /locus_tag="RHW33_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV1647715.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2037 a         2104 c         1921 g         2135 t
ORIGIN      
        1 tagtgacgcc acagcaacaa atataacctt tgtggagcac tatcatgctg aaaccagaaa
       61 tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg ctttatcagc
      121 aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc ctgcgccgcc
      181 acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact gataccggaa
      241 acttaccgcg tattaatacc gttgaatctc cgtttgctga atattcttca cttgatgaat
      301 tattccagga aacctataaa cacgaacaat taatcacgca gaaaattaac gaactggctc
      361 atgctgcaat gaccaatcag gactacccaa catttaattt cctgcaatgg tatgtttctg
      421 agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc ctggcaggca
      481 aaagcggcga aggtctgtat tttatcgaca aagaactctc taccctcgac acacaaaact
      541 aatgctctcc ggcggcaggt tttctgccgc cgtttcaaat cagtgacggc aaatcttact
      601 ctgatcggtc gaaaaaccgt cttcagaagg aataaatttg ccttttgctg ccagaaacgc
      661 caccagctct cccgctgtca tcccttctgc cgagcaggtg tgaaaacgtg cctgttcacc
      721 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
      781 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaaagc
      841 ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga atgcttttat
      901 tgtacattta tatttacacc atatgtaacg tcggtttgac gaagcagccg ttatgcctta
      961 acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag
     1021 aactctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggta tgctggcaat
     1081 gccgctggct gcggcaggtg ttggttttag cgtcacgtta atcttgttga ttgggctttg
     1141 ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga
     1201 taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg
     1261 cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg gtgccggtga
     1321 attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt
     1381 gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt
     1441 atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct
     1501 gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact
     1561 gtctgcaatc ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt
     1621 cagctatatg gatggcaaca ttcgtaagct acgctgggtg tttataaccg gtagtgcgat
     1681 ccccctggtg gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac
     1741 ctttatggga ttgctggcta atcatgccgg attaaacggg ctgctacagg cgttacgcga
     1801 aatggtggcc tctccgcatg ttgagctggc agtgcattta tttgctgatt tagccctcgc
     1861 cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg
     1921 ttcaaatacc gttggtggaa gattgcaaac tggtgcaatt acctttctgc cgccgttggc
     1981 gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct
     2041 ggcggtactg gcattgatta tcccttcgct gttgacctgg caaagcagaa agcacaatcc
     2101 tcaagcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg
     2161 tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg
     2221 attagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatttgact
     2281 tcttaatgca ggcagcactg cttaaatttt ttaccactac cgcacgggca aggatcgtta
     2341 cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc
     2401 atccagtacg catgtagatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac
     2461 gcttctggcg acatcttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc
     2521 gcctccagcg ctggttttaa cgaatcgggc aatgttgacc agtcagaaag tgccacgccc
     2581 cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc
     2641 aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc
     2701 atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccagcgcggc
     2761 acatagtcag ccccacccca cacggcaacc agccactgtg ccggttcaat ctcttgcgga
     2821 gaactcaaca ctgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca
     2881 gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc
     2941 gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc
     3001 cgtatcagag atactttttg agtggctttg ctggtgatta aaaattaagg ggggtgtaac
     3061 gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattgctt
     3121 tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga
     3181 acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa
     3241 agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc
     3301 gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctg acgccgatgt
     3361 cttttaaact ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt
     3421 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
     3481 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
     3541 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattgcgccg aaacgatcac
     3601 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
     3661 accgcagcca caaagaaaag tgcaataccg gcgtactcaa cccaaatgtt cggacgccac
     3721 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagaca
     3781 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
     3841 tcacgggcga tcatcgttgc cgccggtaag gtcacccacc agctgtgata atgctcggtt
     3901 accagcacca tggcgatagc cacgagaact ttatctgcta cagggtcaag gaaagcacca
     3961 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
     4021 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
     4081 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
     4141 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
     4201 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
     4261 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accacccata tatttcaaca
     4321 acatttgccg gcgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
     4381 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
     4441 gctggataac atgcagcgcg ggagaatctg gcggcaaact gaatccctca ccttccggct
     4501 caaagaacag cgtttccagc ccagccttac gatctgctcc tttggcaacg ccaagtagca
     4561 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
     4621 gctggccttt gccgccgtcg ataaggatca catcagggat cttactgtcg tcaatggctt
     4681 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcctgatgc
     4741 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
     4801 aggaagcgac ggtttgttca cccatggtat ggctgatgtc aaagcactcc atccgcttca
     4861 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
     4921 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
     4981 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
     5041 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
     5101 tgcgcatctg gctgccttgt aaatagaact gtcctacgaa ggtttccacc acctcgctca
     5161 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccaagcact ttgccctgac
     5221 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcga
     5281 ggtcgtcgcc ggtattggac acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
     5341 ggtcgcgaat acgtgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
     5401 taatcagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
     5461 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggcccc agacaacgtc
     5521 ctatctgata ttgcagacac ggacgcgagc gattgcgata aacactgttt tcgcactggc
     5581 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
     5641 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
     5701 gggtatcgcc actcaggaag ataaaaggat atgatttatc atcgcgtagc aaaacgttgt
     5761 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
     5821 gagtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
     5881 cgaggttgct acggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
     5941 agataaccgt accaccagca tcgtacatgc gataaacgcc aggctggctg gttacggttt
     6001 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
     6061 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
     6121 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
     6181 ctcattgacc ttctggccct tggtgatcat cagcataatc tgcaattcac gttcagacaa
     6241 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
     6301 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
     6361 ctgcggagcc gcgcctttgc tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg
     6421 taaagggttt tctgtatgga cggtaagcat gatgattttg acatcagctg tggaacgcgc
     6481 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
     6541 atcaacggca tttgcccggc accacttaac ggcgtcttca ccgcacgatg cctcaccgac
     6601 gacttttata ccctttatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
     6661 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
     6721 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
     6781 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
     6841 gtcaccaacg gcttaccgga tcgtttcaca gtaagccctg cgacgaaatg tgacaaaaat
     6901 tacctttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
     6961 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgcatt
     7021 taatagcacc gttgacggca cgctcaaaaa acaaccactg ctatttttag caaagcttat
     7081 ggtatactcc gccgccttaa catttttcac cgcaaatttt cattgcaaca tgacgaggaa
     7141 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
     7201 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
     7261 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
     7321 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
     7381 gctgagagcg tgcctttcag ccggtttttg catctggcac gtaggacaga aaagagatca
     7441 aattaagcca gtagcggccg cgtaacaggc aacctgcgtc ttatttggtg cattaatttt
     7501 tttctgcatg tttttctggt ggaaattgac cgtattctca gagattgaca aaatcatcgc
     7561 tatctctgct gatgtcttcc cttccgccgt ccacctcaga atttcttttt cgcgcttgct
     7621 gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag ccatcagact
     7681 ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg gtatttcgcg
     7741 cgcgctgcaa cgggaaaagg acaaaaagcc cagcgcccgg tttggcagca ttaaatactg
     7801 agtgacaccg cggcgtaaac catgtgcgcg cgcggcttcc cataacggct gtgcttcgtt
     7861 gaataagtca tcattccaca ttagatggcc ctgactaaag ttttcagggt tcagcaccgg
     7921 atcaattgcg agaaagtttt ttgcctgata ataactaacc cacgcctcag ggtaattggt
     7981 gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata acgaatagta
     8041 atcgtactcc agctgctgag cctgaagctc tatttcatgg tagacctctt ctgcggcctc
     8101 catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg tatcctgcat
     8161 agtaaaccgc aacgcccctg agagtgagta tcattta
//
LOCUS       DAPGRN010000165         8196 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPGRN010000165 DAPGRN010000000
VERSION     DAPGRN010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.167"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1759
                     /gene="adeD"
                     /locus_tag="RHW33_004272"
     CDS             <1..1759
                     /gene="adeD"
                     /locus_tag="RHW33_004272"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV1647716.1"
                     /translation="SINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGE
                     ISGPIVIKGRYIAGVGAEYTDAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFET
                     ATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAKQARQNQYLQVSSCVPALEGCDVNG
                     ASFTLEQMLSWREHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGK
                     ELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQC
                     MLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAP
                     GKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSA
                     SDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRLAPA
                     CGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQ
                     SHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLT
                     SQGLFDGKKFAFTTLEVTE"
     gene            complement(1806..3197)
                     /gene="uhpT"
                     /locus_tag="RHW33_004273"
     CDS             complement(1806..3197)
                     /gene="uhpT"
                     /locus_tag="RHW33_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV1647717.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3335..4654)
                     /gene="uhpC"
                     /locus_tag="RHW33_004274"
     CDS             complement(3335..4654)
                     /gene="uhpC"
                     /locus_tag="RHW33_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV1647718.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4664..6166)
                     /gene="uhpB"
                     /locus_tag="RHW33_004275"
     CDS             complement(4664..6166)
                     /gene="uhpB"
                     /locus_tag="RHW33_004275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV1647719.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWMLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6166..6756)
                     /gene="uhpA"
                     /locus_tag="RHW33_004276"
     CDS             complement(6166..6756)
                     /gene="uhpA"
                     /locus_tag="RHW33_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV1647720.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQEPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6918..8021)
                     /locus_tag="RHW33_004277"
     CDS             complement(6918..8021)
                     /locus_tag="RHW33_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647721.1"
                     /translation="MDSYIKIEQTGSNKFKVTDMYREFNDETKCFDKKCRDMDFSGRN
                     VKEFIRLLSDHDQFEKDQISELTVAANALKLEVAKNNYNMKYSFDRQTERRMIELIRE
                     QKDLIPEKYLHQSSIKNLKLDEDEFSSLLADTERQVLEGSSFILCCGEKINGTISELL
                     RKKITDSTHSTKSLTLSESPSYDVYEEIFKKAVFTPDECHLVRDSLVQDGIKPEKIAK
                     IISCLSSRTGIGNLFYACSQAFHVANDNAALDVRRAATSVGDTIDVCPSFVALNGRNN
                     HYHVDFSEYGFTQTVCILRAVVNNANDIFDVANYIYTTKGENIIHNDVEDDSPYVHSD
                     ESYRHKDDYWSRDNDIRNLRRNFSGNITIATYK"
BASE COUNT         2141 a         2229 c         2010 g         1816 t
ORIGIN      
        1 ttctattaac cataaatttc atcacattag ccgggctgaa taccaggaat tgttagccgt
       61 ttcccgtggc gacgctgttg ccgattatat tattgataat gtctctattc tcgacctgat
      121 caatggcgga gaaatttccg gcccaattgt gattaaagga cgttacattg ccggtgttgg
      181 cgcagaatac actgatgctc cggctttgca gcggattgat gcccgcggcg caacggcggt
      241 gccagggttt attgatgctc acctgcatat tgaatccagc atgatgacgc cggtcacctt
      301 tgaaaccgct accctgccgc gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt
      361 caacgtgatg ggcgaagccg gattcgcctg gtttgcccgc tgtgccaaac aggcaaggca
      421 aaaccagtac ttacaggtca gctcttgcgt gcccgctctg gaaggctgcg atgttaacgg
      481 tgccagtttt acccttgaac agatgctctc ctggcgggag catccgcagg ttaccggcct
      541 tgcagaaatg atggactacc ctggcgtaat tagcgggcag aatgcgctgc tcgataaact
      601 ggatgcattt cgccacctga cgctggacgg tcactgcccg ggtttgggtg gtaaagaact
      661 caacgcctat attgccgcgg gtattgaaaa ctgccacgaa agttatcagc tggaagaagg
      721 acgccggaaa ttacaactcg gcatgtcgtt gatgatccgc gaggggtccg ctgcccgcaa
      781 tctcaacgca ctggcaccgt tgatcaacga atttaacagc ccgcaatgca tgctctgtac
      841 tgatgaccgt aacccgtggg agatcgccca tgaagggcat atcgatgccc taattcgccg
      901 cctgatcgaa caacacaatg tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac
      961 ggcgcgccac tttggtctga atcacctggg cttactggca cccggtaagc aggctgatat
     1021 cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag gtactggtga aaggcgaacc
     1081 gattgacgcg caaaccttac aggcggaaga gtcggcgaga ctggcacaat ccgctccgcc
     1141 gtatggcaat accattgccc gccagccagt ttccgccagc gactttgccc tgcaattcac
     1201 gcccggaaaa cgctatcggg tcattgacgt catccataac gaattgatta cgcactcccg
     1261 ctccagcgtc tacagcgaaa atggttttga tcgcgatgat gtgtgcttta ttgccgtact
     1321 tgagcgttac gggcaacgtc tggctccggc ctgtggtttg ctcggcggct ttggcctgaa
     1381 tgaaggcgcg ctggctgcga cggtcagcca tgacagccat aatattgtgg tgatcggtcg
     1441 cagtgccgaa gagatggcgc tggcggtcaa tcaggtgatt caggatggcg gcgggttgtg
     1501 cgtggtacgt aacggccagg tacaaagtca tctaccgttg cccattgccg ggctgatgag
     1561 caccgacacg gcgcagtcgc tggccgagca aattgacgcc ttgaaagccg ccgcccgtga
     1621 atgcggtccg ttacccgatg agccgtttat tcagatggcg tttctttctc tgccagtgat
     1681 ccccgcgcta aaactaacca gtcaggggct atttgatggc aagaagtttg ccttcactac
     1741 gctggaagtc acggaataat aaaaaaaagc ccggcgtcat gccgggcaaa agtcaccagt
     1801 tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc ggcggatttt gcgttcttcc
     1861 atcaccgcca ctatcgccat caggcagata caaccaatcg cggcgatatc cagcgcggcg
     1921 aaggtgcctg cccagccggt aaggccgaat accggcgtcc catcggcaat cattcccaga
     1981 cctaacttgg caaagctgtc accaatcagg taagcaaagg tgcctttaat accatcggca
     2041 gcaccaatcg cttttttagg tacaaaacca acagcagcca caccaatcaa caattgcggg
     2101 ccaaagacca ggaaacccaa cgcaaagaga gaagccagat agatatattc gttgctggca
     2161 tgttgataca caccgagcgt ggcgataatc agcgccagcg cgatgcaggc caccaggcca
     2221 cggcgaccgt tcgccaggtc agagagccag ccccacagca gcgtaccgac cagcgcacca
     2281 gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag agagtttcag ttcctggaac
     2341 gcgtatacgg ttgaccactg gtcgatacca atacgtacca catagaggaa aatgttggcg
     2401 aagcacagca gccagatcac tttgtttttc agcacatact caacaaagat ctgccacttg
     2461 gtcatatcgg tagattctgt ctctttgtcc tcttcgctga tctcctcgcc gaacagttct
     2521 tcagctttgc cgaggccata agattccggg gagtcgctgc cgtaacgcag gccgataaaa
     2581 ccgacaatca gcgcgataat cgacgggaag ataaacatgc cgataacatg accatcgaac
     2641 aggtaatttg cgccgaacag cgccacacct gctgcgcctg caccgccaag gttgtgagaa
     2701 atgttccaga aaccgaggaa ggtcccgcgc ttacgacgcg gcgtccattt ggtgatggtg
     2761 gagtaactgc acgaaccgcc ggtgctctgg aaaaagccgc ttaaggcgta gaaggcaatc
     2821 atcaggaaca ggctaaccga gccgctgccc atactggcac tgaagcccag catacaaatg
     2881 gcagagagga taagcatgaa cggcaagaat tgtttggtgt ttttgccgtc ggcgtagtag
     2941 gaaaccagcg ttttacccac gccataagtg atggagaaac ccaggccgat catccccagc
     3001 tgcgtcatgc tcaacccgta ggtcgaaatc atatcgttct gcgcgatgtt aaagttcttg
     3061 cgaatcaggt acatcgtcag gtagccgata aagaccacca ggtaggattg catgaacggt
     3121 ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca gggtcggctt gcggacctgg
     3181 tttaagaaag ccagcatggg ttactcctga aatgaatacc tgcgtgaggc atgcattgta
     3241 aaaatcagca gcaaatcagg cctgagatag cgtccaggca aaacctggaa aatttcttag
     3301 ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc ttcgcgcggt gcctgggcgt
     3361 tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc gatagagata accacaaaaa
     3421 aaccgctcca gtgccaggta tcgagtactt tcgccagcgg ccaaccagca agcgacgccc
     3481 ccagataagc aaacaagccg acaaaccccg tcgccgcccc tgccgcctct ttgtgtgaac
     3541 actctgccgc cgccatgccg attaacatct gcgggccaaa gacaaaaaaa ccaatagtga
     3601 agaagcaggt tgcctgcatc acgtagctgg caaatggcat cagccacagc gagccgactg
     3661 aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc acggttgccg ttaaacaatt
     3721 tgtccgagcc ccaaccggct accagcgcac cgataaatcc gcccagttca aacatcgtca
     3781 ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc ggacatatac aaattgcccc
     3841 agtcgttgat tgccgcccgg accacataga ccagcacata gcaaaacgaa agcagccaga
     3901 tatacggatt cagtaacaca tacttggtga ggatctcttt acgcgtcagc cctgcccctt
     3961 cttgttgttg agcaatctcc agcgcgtcgt gccgccattc accgaccgcc ggtaaaccta
     4021 acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat ccccacgacg atcgccatac
     4081 aaccagcaat catcatcccg gcacgccagc cgtaatgtag tgcagccgct gccatcacaa
     4141 tgggaatgag tgcgccgcca acgttatgcg ccgtgttcca taatgcccac caaccgccgc
     4201 gctcggtacg tgaataccag gccgttaaca gacgcgcaca caccggtgaa ccccagccct
     4261 ggaaaaaggc gttcagcacc cagagcacgg caaacgccca tagcgacgtc gagaagccaa
     4321 acagaatgtt gatgatgccc gtggcaataa gccctatccc cataaaataa cgggcatttg
     4381 agcgatcgct gacaatgccg gagacaaact tcgacacgcc gtaagtaatg taaaacaggg
     4441 tcgctaacag gccgatatcg ctacggctga gcacgccgtt agcaaggatt tctggtacgg
     4501 cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta acccagccag atggtcagca
     4561 gaatatgccg acgccagtag cgatagcgtg catcaatttc atgtttgtca gtcattaatg
     4621 gcgcatcggc aggcgctttc agaaacggca acatcatcga accttagaca taacgttgag
     4681 gtagagaaac gctgacacgc gtgccgtgca gacaggaaat gtgtaatgtg ccgcccagcg
     4741 ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg ttgcccggaa cccggcggca
     4801 aaccgctgcc atcgtcttca atcaccagca tcaaccgttc atcctgctgc cagccttgca
     4861 gggtgaccgc gctggcatca gcatgtttca caatgttgtt cagcccttcc tggcagacgc
     4921 gaaacagcgt cacgcgctgg ttttcactta acgctgactc atcgatacgc cattcgagat
     4981 ggctgacaat gccgcgccct tccagctcca tttcccgcat cagtgagcgg atggcctgct
     5041 ccagggtgag atcatccagc tggcgcggac gtaaccgccc taacaaacgg cgcaccgcat
     5101 cgtaaacacc cagcgacagt tgttcgatga gctgcccgct ctgcttcacg ctggcgttat
     5161 ctgccgccag tcgctggaca atgcccgcct gagtacgaat ggcagtgatg gtctgaccga
     5221 tatcatcgtg cagctcacgc gccacgtcgc ggcgcacgct ctcttcagtt tctaacaaac
     5281 gttcagccag atgctgattg cgcgctagtt ctttttgcag cgactggtta agttcacgca
     5341 accgctggat acctgcgccc agtaacaacc ctgtcagact ttgcaccagc agcgagagca
     5401 ataagtccac cggatgatcg cgccaggttt gactggcgat cagcgcgatg gcgttcatca
     5461 acgtcgcaat cagcgcccct tgccagccat agtgccaggc cagcgcgata atcggcagcg
     5521 ccaggcagaa tggcgtaaag cgcgacagtt cgtccggcaa tcccaactgg agccagagac
     5581 taataacaaa cagcagcaag taccagacca gatgccgccc gcgccagttg atcggttgag
     5641 aaactaacga cgggccgagc ggcagccagg tgttattggc gagatagtgc cagaacacca
     5701 gacatatagg ggccagcgtc aggccgccag ttaaagtcag tagtagcgca ttccacgact
     5761 ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc cgccgccgtc agggccgccc
     5821 cctgtaacag caaggtgcgc cagtcacgtt gatggcgata gcgcgagatc agcgcaacgg
     5881 gtagtaacgt cagtaaacta ccgatcatca ataaggaaaa atgggttaaa ccgaccgcct
     5941 gcgttagcca gtaaatcagc atccactccg cgcccagcag caccggccaa tatccgcgcg
     6001 ggcattgcag cattagcccc agacgcagac caaacggaaa taacagcacc gccatatcag
     6061 ggcgctcaac caggtgcagg ctgatactcc acaagcaaaa ccatgcggca gagaagataa
     6121 aaaagcaggc aataacggta attaagcggg agaacaacgt cttcatcacc agccatcaaa
     6181 catgcggcgc gccagttcaa cgtcgttact gacacccagt ttttccatca ggttggcgcg
     6241 atggacgtgt accgttttcg gtgacaagcc cagttcggcg gcaatctctt tcaccgccat
     6301 tccttgcgcc agtttttccg ccacctggcg ttcgcgtttg gttagcggtt cctggcgacc
     6361 ggatgccagt ttaatggcaa tatccggcgt cagataacag ccgcccgtgg caaccgtatg
     6421 caccgcagca atcagttcgt cagggctaca acgtttggag agaaagccgc gcgcccccgc
     6481 gttaagcgcc tgctcaacca acgcaggact gtcgtgaacg gagagcataa ttgtcgccat
     6541 acctttcggc agctggctta gcaactccag accggagata tcgggcatgg agatatcgca
     6601 aatacacacc tgcacaccac gccccggcag ccccgccagc gcctcgcgcc ccgaaccaaa
     6661 ctcggcaact acctgcaaat caggttccag ccccagcaac tgcgcaaagc cggagcggac
     6721 gataaggtga tcgtctataa gggcaacggt gatcatgatt gggtcctggc ggataaaaaa
     6781 cgcgcatacc ttaacgataa gcgcgatgtt gttcaagttg gatatggggt atgtattggc
     6841 ttaatgatgt tgcgcttatc gtcaaaacgc ttacctgaga ggtaagcgtt tggtgcgtgt
     6901 tattgagaaa aagcaaatta tttgtaggtt gcaatcgtaa tattgcctga gaaatttctc
     6961 cttagattgc ggatatcatt atcccttgac caataatcat ctttgtgtct ataactttca
     7021 tcggaatgta catagggaga atcatcttca acgtcgttat ggataatatt ttcgccttta
     7081 gtggtataga tataattagc cacatcaaag atgtcattcg cgttatttac gactgctctt
     7141 aagatgcaaa cggtttgtgt gaatccgtat tctgagaaat cgacatgata atggttattt
     7201 ctaccattca aagcaacaaa actcggacaa acatctattg tatcgccaac agatgtagcg
     7261 gcgcgacgca catcaagtgc agcgttatca ttagcaacat gaaatgcttg tgaacaggca
     7321 taaaacaaat ttccaatccc ggtacgactg gataaacaag aaataatttt tgctattttt
     7381 tctggcttta taccatcctg cactaaagaa tctctcacca gatgacattc atcaggtgtg
     7441 aaaacagctt tcttgaatat ttcctcataa acatcataag aaggagattc agagagagtg
     7501 agtgatttgg tggaatgtgt tgaatctgta atttttttgc gaagtaattc acttatagtg
     7561 ccgtttattt tttcaccaca gcataaaata aatgaacttc cctccagaac ttgtctttct
     7621 gtatctgcaa ggagtgagct aaattcatcc tcatcaagct taaggttttt tatagatgat
     7681 tgatgtaaat acttttctgg aattaaatct ttttgttccc ttataagctc tatcattcgg
     7741 cgttcggttt gcctatcaaa agagtatttc atgttatagt tatttttagc aacttccagc
     7801 ttaagagcat tagcagcaac tgttaattct gagatttgat ctttttcaaa ttgatcgtga
     7861 tctgataata accttataaa ctcttttaca tttcgaccac taaaatccat atccctacac
     7921 tttttatcaa aacactttgt ttcgtcattg aactcacgat acatatcagt gactttaaat
     7981 ttattacttc cggtctgctc aatttttata tagctatcca tgcctgtaac ggcaatccat
     8041 gtcttataat tcgcattctc cgattcaggc gtaacatgaa aataaggaac caatttatca
     8101 ctaatatcat gccgatagat atgaaatgaa tctaaaagat ataataaaaa acttcgaaaa
     8161 atgtcacgaa aagtgtcatc tttaaacctt gcccta
//
LOCUS       DAPGRN010000166         8159 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPGRN010000166 DAPGRN010000000
VERSION     DAPGRN010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.168"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..284
                     /locus_tag="RHW33_004278"
     CDS             <1..284
                     /locus_tag="RHW33_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647722.1"
                     /translation="TNELTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNS
                     TYIYKSRHEILPVNLTPETLFGSKSQDKHALFPIFTASWRANRIKNKGI"
     gene            507..1004
                     /gene="ubiC"
                     /locus_tag="RHW33_004279"
     CDS             507..1004
                     /gene="ubiC"
                     /locus_tag="RHW33_004279"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV1647723.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1017..1889
                     /gene="ubiA"
                     /locus_tag="RHW33_004280"
     CDS             1017..1889
                     /gene="ubiA"
                     /locus_tag="RHW33_004280"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV1647724.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2044..4467)
                     /gene="plsB"
                     /locus_tag="RHW33_004281"
     CDS             complement(2044..4467)
                     /gene="plsB"
                     /locus_tag="RHW33_004281"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV1647725.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPTHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4638..5006
                     /gene="dgkA"
                     /locus_tag="RHW33_004282"
     CDS             4638..5006
                     /gene="dgkA"
                     /locus_tag="RHW33_004282"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV1647726.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5116..5724
                     /gene="lexA"
                     /locus_tag="RHW33_004283"
     CDS             5116..5724
                     /gene="lexA"
                     /locus_tag="RHW33_004283"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV1647727.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5797..7122
                     /gene="dinF"
                     /locus_tag="RHW33_004284"
     CDS             5797..7122
                     /gene="dinF"
                     /locus_tag="RHW33_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV1647728.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLFGLLMVRKILKLRGI
                     SGEMLKAAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFSAT"
     gene            7238..7447
                     /locus_tag="RHW33_004285"
     CDS             7238..7447
                     /locus_tag="RHW33_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV1647729.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7489..8004)
                     /gene="zur"
                     /locus_tag="RHW33_004286"
     CDS             complement(7489..8004)
                     /gene="zur"
                     /locus_tag="RHW33_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV1647730.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1839 a         2004 c         2236 g         2080 t
ORIGIN      
        1 acacgaatga attaacacga aacgataata tctttatctt gttctcctct gtcgatgatg
       61 ggccagttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct acaaaagata
      121 ccaattggaa ttccacgtat atctacaaat ccagacatga gatattgcct gttaatctta
      181 ctccggaaac acttttcggc tccaaatctc aggataaaca tgcacttttc cccattttta
      241 ctgcgagttg gcgagctaat cgtataaaga ataaaggtat ttaagtaaag gaaaacatca
      301 ccgttcctgg catcctgggc ggtgatgccc ctatggttgc cctcgccagc acgggcatcg
      361 gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt ttttgttgcg
      421 cattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga tacaatgcct
      481 ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa ctgcgtgcgc
      541 tgcgctattt taaagagatc cctgccctgg agccgcaact gctcgactgg ctgttgctgg
      601 aagattccat gacaaaacgt tttgaacagc agggaaaaac ggtgagcgtg acgatgatcc
      661 gcgaagggtt tgtcgagcag aatgaaattc ccgaagaact gccgctgctg ccgaaagagt
      721 ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg cttgccggtc
      781 gtaccgtcgt tccggtgtca acgttaagcg ggccggagct ggcgttacaa aaattgggta
      841 aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac tttattgaga
      901 taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc ggtaaaccgc
      961 tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa aaaaatatgg
     1021 agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt acggataagc
     1081 caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg gcgacaccgg
     1141 gcgttcccca gctctggatc ctggcggtgt ttgttgcggg tgtctggctg atgcgcgctg
     1201 ctggatgtgt ggtgaatgat tatgctgacc gcaagtttga tggtcatgtt aagcgcacgg
     1261 cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg ctgtttgtag
     1321 tgctggtact gatttcgttt ttactggtgc tgacgctgaa tacgatgacc attctgttgt
     1381 cgattgccgc gctagcgctg gcgtgggtgt acccgtttat gaagcggtat acccatctac
     1441 cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt gctgctgtga
     1501 gtgagtcggt gccattgagt tgctggttaa tgttcctcgc caatattctc tgggcggtgg
     1561 cttacgacac gcagtatgcg atggttgacc gtgatgatga tgtgaagatt ggcattaaat
     1621 ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg cagattggcg
     1681 tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga tattactggt
     1741 caattctggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc aaccgcgagc
     1801 gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta ctgtttttag
     1861 ggctggcaat gagttactgg catttctgat gatgtaaaaa agccggatga tcatccggct
     1921 ttcttctggg ttgcctgatg cgctgcgctt ctcaggccta cacaacagat cgcaatttat
     1981 tgaatttgca gattatggaa ggccggataa ggcgtttgcg ccgcatccgg caattctctc
     2041 tgattaccct tcgccctgcg ttgcactctc aatcgtcaaa cgcacgtctg atgtaatcaa
     2101 ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcatcgc cgctatcgct
     2161 gatataccct tcatcacgta gcgtcagcac cagagaactg aacaccgcct tgtcgaagaa
     2221 ctccggcgcg ttgataccgt gcagcacgga gagacgttgc gcgacggtgc ggctctcttt
     2281 ctccagcgta ccgcggttga tcgacgggtt ggcgctcaac agccagaagg tgatggcata
     2341 acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agcgtgcgag aatgcgtcgg
     2401 gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca tctcatttgc
     2461 cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca gctccgcttt
     2521 cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga tgtggcgatg
     2581 ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa tgttgttgcg
     2641 atagtaggtc atcagcaccg cttgctcgcg cggcagaatg atgatgtcgc cgatagtgtc
     2701 tttctcaact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc tggcgctggc
     2761 tgaaggaacg gtagagtccg tggagtaagg cacgttgcgc atcagatcca ggtagcagtt
     2821 gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca gtagcgcagt
     2881 acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca tcagatcggc
     2941 agcaatatta ttgaccgtcg gtgttaacca tgccggacgc accgcttcga tgggatcgat
     3001 agattcacgc cagtcaggta cgtgctggtt aaggtaggtc atcaacggca ttggttcacc
     3061 gaaattgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca gcatctgcgg
     3121 caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac ccacttccat
     3181 gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc cgccacgcag
     3241 catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac gccccgtacg
     3301 ggaacgaccg ccttctacga agtactcgac ggaataacca cggctgaaca gttcgccgag
     3361 atactcacgg aaaacggtgg aatagagctt attgccttta aacgtacggc gaataaagaa
     3421 cgcacccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga tcccggcggc
     3481 gatatgcggc ggcaccagcc cctggtgata cagcacgtaa gaaagcagca ggtagtccat
     3541 gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca actggcgaac
     3601 gcgctcggcg ttatggacgt tgataccctg gtaaagtcgg ttccaggtga agcccagaat
     3661 acggtcagtc aggcgaatca tctcgtaaga gaaattcgcc gcaatctctt ccatcagtgc
     3721 aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt catcttctac
     3781 cgctttcgca atggcgcggg aggcgagcag cttattaaac agatcctgac gagcaggcaa
     3841 acgtgggcct acggcagcca gacgttgacg ggcaaagtgc atacgcgcca cgcgcgccag
     3901 tttctgcgcg atagttttat ccgtgccgtg ttcatccgcc atacggcgca gcgaaactga
     3961 cggtgagaaa cgcacaaaac tgtcgcgacc gagccacagt acagcgaaaa atttctgtac
     4021 gccgttaagc atacgcagcg gcgggttcac ttcgcctttt tcacgccccg gcgcgcgacc
     4081 aaacatcacc gacactggca ccatctgcac atccagattt gggttgctac ggtgcaaatc
     4141 gagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg tgaatacacg
     4201 cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt ccagcggctc
     4261 taacgggtca ggcaagtcat gtgccagaca ctgggcgcgc aacgtcagca aatctgcttt
     4321 cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca gttccggggc
     4381 aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat tcagtaattt
     4441 gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta ataatgcaaa
     4501 tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc atgtttcgcg
     4561 cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga cgctcataat
     4621 gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaata atcaaagctg
     4681 ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca ttccgtcagg
     4741 aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg gacgcgatta
     4801 cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc ctcaatagcg
     4861 ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc ggacgcgcta
     4921 aagatatggg atccgccgcg gtgctgattg cgattatcgt cgccgtgatt acctggtgca
     4981 tcctgttatg gtcgcatttt ggataaccct tccagatttc gataaatctc tggtttattg
     5041 tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata actgtatata
     5101 cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt tgatctcatc
     5161 cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc gcagcgtttg
     5221 gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg caaaggcgta
     5281 attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga ggaagaaggg
     5341 ttgccactgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca gcatattgaa
     5401 ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct gctgcgcgtc
     5461 agcgggatgt cgatgaaaga tatcggcatt atggatggcg acttgctggc agtgcataaa
     5521 actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga agttaccgtt
     5581 aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag cgagtttaaa
     5641 ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc ggttggcgtt
     5701 attcgcaacg gcgactggct gtaacatatc tctgagaccg cgatgccgcc tggcgtcgcg
     5761 gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac ttcatctgat
     5821 aaagcacttt ggcatctcgc cttacccatg attttctcca atatcaccgt tccgttgctg
     5881 ggactggtcg atacggcggt tattggtcat cttgatagcc cggtttattt gggcggcgtg
     5941 gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt acgcatgagc
     6001 accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt agcccgtgcg
     6061 ctggtgcaac cgttgctgtt ggcgttgggg gctggggcgt taattgcgct gctgcgtaca
     6121 ccgattatcg atctggcgct gcatattgtt ggcggtagtg aagcagtcct ggaacaggcg
     6181 cggcgctttc ttgaaatccg ctggttaagc gcaccggcgt cgttggcgaa tctggtatta
     6241 ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt agtggtcggc
     6301 aatatcctca acattgtgct ggatgtctgg ctggtaatgg ggctgcatat gaacgtgcag
     6361 ggtgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgtttgg tctgctaatg
     6421 gtgcgtaaga tcctcaaact acgcggaatc tccggcgaaa tgctgaaagc tgcctggcga
     6481 ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgctc gctgttgttg
     6541 caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga cattatcgct
     6601 gtaaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgctgga tggttttgcc
     6661 tacgcggttg aagcgcattc cggtcaggcg tacggtgcgc gcgacggtag ccagttgctg
     6721 gatgtctggc gggcagcgtg ccgccagtcg gggatcgtag cgttactgtt ttcggtggtt
     6781 tatttgctgg caggggaaca catcattgcg ttgctgacgt cgttaaccca gattcagcag
     6841 ctggccgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt ctggtgttat
     6901 ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa cagtatggcg
     6961 gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg caatcatggt
     7021 ttgtggctgg cgttaaccgt ctttctggcg ttgcgcgggc tttctctggc ggctatctgg
     7081 cggcgtcact ggcgcaatgg tacctggttt tccgcaacgt gacggttaaa aattctgaat
     7141 aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag aaaagtgaac
     7201 gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg aagccggcgg
     7261 taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga ccgatgatga
     7321 tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg aacgttatgg
     7381 ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca atgaatatcg
     7441 ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt aacgcggttt
     7501 ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac acgcttccac
     7561 ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca gggcaaaccc
     7621 catttttgcc gccagcgtat gcatgatgtc ttccacgcct tcagcacatt cttctttcac
     7681 cgcgccgcag cgatcgcaaa taaacatagc tgacgtatgg gtgggctggt cgaacagatg
     7741 gcagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct caagcagaaa
     7801 atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc gcagcaaatc
     7861 aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca acacttccag
     7921 gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct gcgctaataa
     7981 ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga atgtccttac
     8041 tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg ttgattttct
     8101 gttttgtacg taaggtgttg tgttgcctgg tcgtaccatt atcaacacga taataatta
//
LOCUS       DAPGRN010000167         8068 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPGRN010000167 DAPGRN010000000
VERSION     DAPGRN010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.169"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            471..1538
                     /gene="pilL"
                     /locus_tag="RHW33_004287"
     CDS             471..1538
                     /gene="pilL"
                     /locus_tag="RHW33_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis lipoprotein PilL"
                     /protein_id="HDV1647731.1"
                     /translation="MKKNYLTLILPSLLSGCAGISGSNNVAPSDTPALVFVDGQISES
                     AGVLALTQQRLSPPKITPPPRVSVPETVKTAHPVSESRPLPSPTISQNFSRLIITGKP
                     GSAPVTLSPTARNRTVSQWIKTLLPEGWKFRQENAATPKLNTKLVSWSANDQWTRSLN
                     RLLEEQHLWGHLDWDNKTLTVTTSAAAPAQDTATTISPAAMVTTSTSPESPTTANSQN
                     KPRNPFRGNSVSSSTPAATPTPTGSTVKSIPLMTGTPVKPVSQGKEWRAPAGTTLREN
                     IIKWAEETKCESMASTHWMVIWPLSVTDYRLDAPLMFRGSFESAMEQVFELYRTAQKP
                     LYAQASRMQCIITVSDTRDGR"
     gene            1538..1975
                     /gene="pilM"
                     /locus_tag="RHW33_004288"
     CDS             1538..1975
                     /gene="pilM"
                     /locus_tag="RHW33_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HDV1647732.1"
                     /translation="MGWLVMAVGLTILIITGSYQNQKMSETTNAQQYASASVWASQIL
                     MIANRINDIRYVSGQQDGVISSDKLALPVTPDSRIKHQLQQGRLWVWMPEQPGLVETL
                     RSKSRGSALIGIFQNGQLTWLSGTATGLTPPAGITAGSVVYVN"
     gene            1989..3671
                     /locus_tag="RHW33_004289"
     CDS             1989..3671
                     /locus_tag="RHW33_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HDV1647733.1"
                     /translation="MKKSHQRSMKLAVLPCMIAVALSISGCTFSEINKMQKKAQEDSA
                     HAREKVSALSARKSQALTWLDNQWINPVPVAQVSREKKQTAPACYITQARKGEITLQE
                     LGQRITAVCGIPVIITPDAANSTLEGGATRQMTGTLPAPDENGRLPLSSLGSTTMTTS
                     TQPLTLNNLMWQGDINGLLDLMASRSGLYWRMDNSRIVFYLTETRTYPLHMLNTKTSS
                     SSSVSSGSTSTMGATGGQDNSASGDATSSQSTTVGQEYDLYEDIRKTIEAMLTPEKGR
                     YWLSASSSTLTVTDTPAVQEAVARYVDEQNSIMNRQVALNVQVLSVSNTRNEQFGLDW
                     NLVYKSLHSAGATLNNASGDFTGATSAGVSILDTATGNAAKFSGSSLLIKALSEQGDV
                     SVVTSQESTVTNLTPVPIQMADQTVYVAQSATTTTTDVGATTTLTPGMITTGFNMTLL
                     PLIQKTGNLQLQMNFNLSDPPTIRSFTSKDGNSYIEMPYTKLRSLSQKVNLKEGQSLV
                     VTGFDQNNTTTSKAGTFTPANPLFGGSQTGKNERSTLVIIITPTFPSGGNNG"
     gene            3664..4959
                     /gene="pilO2"
                     /locus_tag="RHW33_004290"
     CDS             3664..4959
                     /gene="pilO2"
                     /locus_tag="RHW33_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HDV1647734.1"
                     /translation="MADEDINPVVLLADPKVNHRVWAACLKWSPVVKKQRVPSHQKHK
                     PHVKSRRLTSLKVTVGSRSSRGKISRITGTGILARPERNHYFSLALAFCSWVRNGYGV
                     FRYSDKELLFLASINGQPAVMADLSGNDADVAQKVSLFLTMNEEPPEKWQVVSPLEHP
                     DNWESIITRLSSADLRRCKLTVGNRSKFTLPAVLFLVAASAGTVFWMTQPEPDVVPTP
                     EEIAARARLQFKKPDPPPELPHPWASQPVISDFLKACADLRKPSPVALEGWKLTGGTC
                     TPETFTLIYERQPGGTIEGFLARSKEIFNVIPDFNLKDGARLASVTRPLPSLPRRDEA
                     VPTPSEQLMRVFTWFQKKQLTPAINEIAIPEPLPGNDGEPAPVQKWKEYQFSLSTPVN
                     PDELFPLFQDTGVRLSNIHFELNGGTFSYSSEGHIYASR"
     gene            4946..5398
                     /gene="pilP"
                     /locus_tag="RHW33_004291"
     CDS             4946..5398
                     /gene="pilP"
                     /locus_tag="RHW33_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HDV1647735.1"
                     /translation="MRPGKLLIIPSIFFFSGFSFATTQPLVTIGELEAQQNRNILLQA
                     KVQGAQLQKQLEESDVTSSSETVSGFSGVTASLPSVSEQPTSKNRKELPVIMEINGKD
                     KRLNAVLRMADGRQTSVTTGSQLPGTSVTVKSISLSGVTLSDGTTLTF"
     gene            5409..6962
                     /gene="tadA"
                     /locus_tag="RHW33_004292"
     CDS             5409..6962
                     /gene="tadA"
                     /locus_tag="RHW33_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDV1647736.1"
                     /translation="MDKYNLKDALLFISRGDTHEILIETNQRTRPDVQSNLQELLKLY
                     PDINPKVVSLSELQEAGQDEKNRGPKERIIHLKDLADVSESEKKVLSYFETARKLGAS
                     DIHFLISESIFKVRMRIFGELQTVDEDQPALGYSLCATAILSMADVTETSFFPQREQD
                     ARLSPQLMRKIGIFGARYSHRPTGDGLIAVMRLIPDDGDKVPTFKQLGFIPEQIRLLN
                     IMLRRPEGKIVLSGPTGSGKSTTLRSACRVYLDDNQGRHLLTIEDPLEGQILGATQTP
                     IICDKSDEDAVKLAWSRAISSAMRLDPDAIMEGEMRDLISMMSTTYAAQTGHIVLTTL
                     HTNSALGIPERMITMGMNADLICDAQLLIGMISQRLVPTLCPSCRIPWEKRAPELSDD
                     ERDYLERHCNKDSLCSTDNIWFRNHHGCSECNHDVIINGRKRGEIGKGLTGRTVIAEV
                     IEPDNRLFQILKTRGKVAARKYWLENMKGISRVEHLLRRINEGLVDPLEADRIIPLDE
                     DERLSIDDV"
     gene            6975..>8068
                     /locus_tag="RHW33_004293"
     CDS             6975..>8068
                     /locus_tag="RHW33_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001699973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDV1647737.1"
                     /translation="MREMNFSQRLRRFIVRKTFSAPYRVQFYEALRFLLENKQPLKTA
                     LEQMRDAWTDFGRKWHPFAELATDCIESLRENSGENSLEYTLSLWVPQEEAAVISAGI
                     RSGSIVDALQFATTLTDAKEQIHQAIWQMAIYPVGLLIMMTGTLYVLNTELIPELSKI
                     SSPDSWSGALGFLYGLSVFVDNYGAICAVLFAVITGLISWSLPNWKSPDSVRTFADKI
                     MPWSIYQDIQGATFLLNMAALLKAKMTTLNSLNILQEFASPWLSTRLDSIIYRVRQGD
                     HLGLALRQCGYQFPSREAANFLSLLQGDGATELISNYGQRWLSQTLQRVKKRANVIRL
                     IMLIFLVMSLMLLVFAIMDIQSISDNSMGN"
BASE COUNT         2312 a         1990 c         1820 g         1946 t
ORIGIN      
        1 tttccccact acttttcaga tgcgtgcttc ttatcgtaag gtctgtatct aaatacaact
       61 tcacattctt tgcgaaaaaa aatggcagta aagaatcaag catacatctt agcccctatg
      121 ccgatacacg ttgcccggag tcaatgagga aacactggga tgtgttactt tctctgatat
      181 caaaagcaat taataatgat gaaaccattg ttatgcattc acatctcttt actcctgcaa
      241 gaataaataa acttataaag agactggaat cttatggttt gaatttctct gttattacat
      301 gcccacggaa aacaagtctg tatgaaagat tatcaattcc ttttgtatat ctcttatttc
      361 agtggtctct cccgcatttg cattcggacg gaatgacagt cgtcattcgt cctctccagc
      421 cgcctcacac caattaacaa acacgtatat acgaacacgg agtctccgtt atgaaaaaaa
      481 actatctgac acttatactg ccgtcattat tgtccggctg tgccggaatt tccggcagca
      541 ataacgtggc accatcagat acgcctgctc tggtttttgt tgacggacaa atcagtgaga
      601 gtgctggcgt tctggcactt acgcaacaac gtctttctcc acctaaaata actccccccc
      661 cgagagtcag cgtgccagag acagtaaaaa cagcacatcc agtcagcgaa tcccgaccgt
      721 taccttcacc aacaataagt cagaatttct cccggttaat cattacaggc aagcctggta
      781 gcgcccctgt aacactatcg ccgacggccc gtaaccggac cgtcagtcag tggataaaaa
      841 cattactacc ggaaggatgg aaattcagac aagagaatgc agccacgccc aagctgaata
      901 ccaaactggt ttcatggtct gccaatgatc agtggacccg ttctctgaat cggttgctgg
      961 aagaacaaca cctatggggc catctggact gggataataa aacgttgacc gtcacaacat
     1021 cagctgctgc accggcacag gacacggcaa ccactatctc gccagcagcc atggtaacca
     1081 catccacttc ccctgaatct ccaacaacag caaatagcca gaacaaacca cgaaatccat
     1141 tcagaggaaa cagcgtatct tcgtcaaccc ccgccgccac cccgactcca accggctcca
     1201 cagtcaagtc tatcccgctc atgaccggaa cgcctgttaa accggtatct cagggtaaag
     1261 agtggcgtgc gccggcaggc accacattaa gagagaacat tatcaagtgg gcagaagaaa
     1321 caaaatgcga aagcatggca tcaacgcact ggatggtcat atggccactt tctgtaaccg
     1381 attaccgtct ggatgcgcct ttaatgttcc ggggatcatt tgaatcagcc atggagcagg
     1441 tatttgagtt atatcggact gcgcaaaaac ctttatacgc acaggcttct cgcatgcagt
     1501 gcatcattac agtttctgat acaagggatg gtcgctaatg ggatggctgg tcatggccgt
     1561 cggcctgacc attctgatta tcactggtag ctaccagaat cagaagatgt ctgaaaccac
     1621 aaacgcccag caatatgcaa gtgcgtctgt atgggcaagt cagatactga tgattgctaa
     1681 cagaatcaat gatatcagat acgtatcagg ccagcaggac ggagtaattt cctcagataa
     1741 actggctctc cctgtcactc cggactcccg aattaaacat cagttacaac aggggcgtct
     1801 gtgggtatgg atgcctgaac aaccaggtct tgttgaaact ttgcgttcaa aaagtcgtgg
     1861 ttctgcacta atagggatct tccaaaacgg gcaactcacc tggctatcag gaactgcaac
     1921 cggtctgaca cccccggcag gcattactgc tggttctgtc gtctacgtta actaatctgg
     1981 atatattcat gaaaaaatca caccagcgtt caatgaagct ggcggtgctc ccctgcatga
     2041 tcgccgtcgc tctttccatc tccggatgta ctttcagcga aatcaacaaa atgcagaaaa
     2101 aagcacagga agactcagca catgcacggg aaaaggtatc agccctttcg gcccgtaaat
     2161 cgcaggctct tacctggctc gataatcaat ggataaaccc tgttccggtc gctcaggtat
     2221 caagagagaa aaaacaaaca gctccggcct gctacatcac gcaggcaaga aaaggagaga
     2281 tcactctgca ggaactgggg caacgtatta ctgccgtatg cggcatccct gtgatcatca
     2341 cgcctgacgc agccaattca actcttgaag gaggcgctac ccgccagatg acaggaacac
     2401 taccagcacc agatgaaaat gggcgtctac cgttaagcag cctgggcagc acaacaatga
     2461 ctacctccac tcagccatta acgctgaata acctcatgtg gcagggagat atcaatggtc
     2521 ttctggatct gatggccagc cggagtggtc tgtactggcg catggataat agtcggattg
     2581 tattctatct gactgaaacc agaacgtatc cacttcatat gctgaacacc aaaaccagca
     2641 gcagttccag tgtcagctct ggctcaacaa gcacaatggg ggcaacagga ggccaggata
     2701 actcagcatc cggtgatgca acgtcctctc agagcacaac cgttggtcag gaatacgatc
     2761 tgtatgaaga catccggaaa actattgaag caatgctgac accagaaaaa ggccgttact
     2821 ggttatctgc atcgagctca acgctgactg tcactgatac tccagctgtc caggaagccg
     2881 tcgcacgata tgtggacgaa caaaacagta ttatgaaccg ccaggtagcc ctgaacgtac
     2941 aggttctgag cgtcagcaat accagaaacg aacagttcgg tctggactgg aaccttgttt
     3001 ataaatcgct acattccgcc ggagcaacgt tgaacaatgc aagcggagat tttacaggcg
     3061 ctacatctgc aggcgtatca attctggata cggcaacagg gaatgccgcc aaattcagcg
     3121 gttccagtct tctgattaaa gcgctgagtg aacagggcga tgtcagtgtt gtgacttcac
     3181 aagaaagcac tgtcacaaac ctgacgccgg tacctatcca gatggcagat cagacggttt
     3241 acgtcgccca gtcagcaaca acaacgacta cggatgtagg agcaacaaca acattaacgc
     3301 cgggcatgat caccaccgga ttcaatatga ccctgctgcc tttaattcag aaaacgggca
     3361 atctccagtt gcagatgaat tttaatctgt cagatccccc aacaatccgt agctttacgt
     3421 caaaagacgg aaacagttac atcgaaatgc cgtataccaa actgcgttca ctgagccaga
     3481 aggtcaatct gaaagaaggg caatcacttg tcgttactgg tttcgatcag aacaatacga
     3541 cgacaagtaa agccggtacg tttacgccag caaatccatt atttggtggt tcacaaaccg
     3601 ggaaaaatga acgcagcacg cttgtaatca tcattacccc gactttcccg tcaggaggca
     3661 acaatggctg atgaagatat caacccggtt gtacttctcg cagatcctaa agtaaatcat
     3721 cgcgtgtggg cagcctgtct taaatggagt cctgtcgtaa aaaaacagcg agtcccttct
     3781 catcagaaac acaagcccca tgtaaaatcc agacggctta cctctctgaa agtgactgta
     3841 ggcagcagaa gttcccgtgg gaagatcagc cgcattaccg gcacaggaat actggccagg
     3901 ccggagcgca atcattattt ttcccttgct ctggctttct gctcatgggt gagaaatgga
     3961 tacggcgtct tccggtacag cgataaggag cttttattcc ttgcctccat taatggccag
     4021 cctgccgtta tggctgatct gtcaggaaat gatgctgatg ttgctcaaaa ggtcagtctt
     4081 tttctgacga tgaacgaaga gccccccgaa aaatggcagg tcgtatctcc tctggaacat
     4141 cctgataact gggaaagtat tattacccgg ttatcatcag cagatctccg ccgttgcaaa
     4201 cttactgtcg gcaatcgcag caaattcaca ctgcctgccg tacttttttt agtagcagcc
     4261 agcgcaggca ctgtattctg gatgactcag ccagagccgg atgtcgtacc gacgcccgag
     4321 gagatagctg cacgggcaag attgcagttt aaaaagcccg atccaccgcc agaactcccg
     4381 catccatggg catcacaacc tgtaatcagt gattttctga aagcctgcgc ggaccttcga
     4441 aaaccatcac cggtcgcgct agaaggctgg aaacttaccg gtggaacatg tactcccgag
     4501 acattcacac tgatttacga acgacagcca ggaggaacta ttgaaggctt tctggcaaga
     4561 agcaaagaaa tctttaatgt tattcctgac tttaacctta aagatggtgc acgcctggct
     4621 tctgtaaccc ggccactgcc ttccctgccc cgcagggatg aggcagtccc taccccctca
     4681 gaacaactga tgcgggtgtt cacgtggttt cagaaaaaac agttaacgcc ggccattaat
     4741 gaaatagcca taccggaacc attaccaggt aacgatggtg aaccagcccc tgttcagaaa
     4801 tggaaagaat atcagttcag tttatcaacg cctgtaaatc ctgatgagtt gttcccttta
     4861 tttcaggata ccggcgttcg tctgagcaat atccattttg aattaaacgg cggtactttc
     4921 agttattcct cagaaggtca tatttatgcg tccaggtaaa ttactcatta ttccatccat
     4981 atttttcttc tccggttttt catttgcaac aacacaacct cttgtcacaa taggagaact
     5041 tgaagcacaa caaaaccgga atattctttt acaggcaaaa gttcagggcg cacagctaca
     5101 gaaacaactc gaagaatcag atgttacttc atcatctgaa actgtttcag gtttctctgg
     5161 tgtgactgcc tctttgccat cagtttcaga gcagccgaca tctaaaaaca gaaaggaatt
     5221 gcccgttatt atggaaataa acggcaaaga taaacgtctt aatgccgttc ttcgcatggc
     5281 tgatggacgt cagacaagcg tcaccactgg cagccagctt ccaggcacat ccgtcacagt
     5341 gaagtcgatc tcattgtctg gcgtcacgtt aagtgatggc accaccctta ctttctgagt
     5401 aacgtgatat ggataaatat aatctgaagg atgctctcct ttttatatcc aggggagaca
     5461 cccatgaaat tcttatagaa acaaaccagc ggacaaggcc tgacgtacaa agtaatctcc
     5521 aggagctgct aaaattatat ccggatatca acccaaaagt agtctcgttg tcggagttac
     5581 aggaggcagg tcaggatgag aaaaacagag gcccgaaaga acgaattatt catctgaaag
     5641 accttgctga tgtcagtgaa agtgagaaga aagttctatc ttattttgaa actgccagaa
     5701 aacttggtgc ttcagatatt catttcttaa tttcagagtc tattttcaaa gttcgcatga
     5761 gaatattcgg tgaactgcag actgtagatg aagatcaacc agcactgggc tattcacttt
     5821 gcgcaacagc cattctgtca atggctgatg tcactgaaac cagtttcttc cctcaaaggg
     5881 agcaggatgc gcgcctgtcc ccacaattaa tgcgaaaaat aggaatattt ggtgcacgct
     5941 acagtcacag acctacggga gacggtctga ttgcagttat gcgcctgata cccgatgacg
     6001 gagataaagt tcccacgttt aaacaactgg gctttatccc ggaacaaatc cggttgctga
     6061 acataatgct gcgccgcccg gaaggaaaaa tagtcctgtc aggtccaacc ggttccggta
     6121 aatcgacaac gttacgcagc gcctgccgtg tatatcttga cgataatcag gggcgacacc
     6181 tgctaacaat cgaagatccg ctggaaggac agatactggg agcaacacag actccgatta
     6241 tctgcgacaa atccgacgaa gatgccgtca aactggcatg gagtcgggct atttcatcgg
     6301 caatgcgact tgaccctgat gccatcatgg aaggggaaat gcgcgatctt atctcgatga
     6361 tgtcaaccac ctacgcggca cagaccgggc acatagttct gacgacatta catacaaact
     6421 cagctctggg tataccggag cgaatgatta cgatgggtat gaatgccgat cttatttgcg
     6481 atgcgcaatt actgattggg atgatcagcc agcgccttgt accaactctg tgtccctcct
     6541 gccgtattcc atgggaaaag cgagcacctg aacttagcga cgacgagcgt gattaccttg
     6601 agcggcactg caataaagat tcactctgca gtacagataa tatctggttc cgaaatcatc
     6661 acggatgctc tgaatgtaat cacgacgtaa tcattaacgg cagaaagcgc ggtgaaatag
     6721 gtaaaggcct caccggcagg actgtaattg ctgaggtgat agaacctgat aaccgcttat
     6781 ttcagattct gaaaacacgc ggcaaagttg cagcacgtaa atactggctg gaaaacatga
     6841 aaggcatcag tcgtgtagag catcttcttc gccgtatcaa tgaaggtctg gtagaccctc
     6901 ttgaagccga cagaatcatc cctctggacg aagacgagcg cctcagcatc gatgatgtct
     6961 aacaggagaa caacatgcgt gaaatgaatt tcagtcaacg gctgaggcgt ttcattgtca
     7021 gaaaaacgtt cagcgccccc tacagggtgc agttttacga agccctgcgt tttctgctgg
     7081 aaaataaaca gccattaaag accgcactgg aacagatgcg ggatgcctgg acagactttg
     7141 gacgaaaatg gcatcccttt gctgaactgg ccacagactg cattgagtct cttcgtgaaa
     7201 acagtggcga aaactctctg gaatatactc ttagtttatg ggttcctcag gaagaggccg
     7261 cagtaatcag cgccgggata agaagtggtt caattgtgga tgcactgcaa ttcgccacca
     7321 ctcttacaga tgcaaaagaa caaatccatc aggctatatg gcaaatggct atttatccgg
     7381 tgggattgtt aatcatgatg acggggacat tatatgttct gaatacagag ctaatacctg
     7441 aactgagcaa aataagctcc cccgattcat ggagtggtgc tcttggtttt ctttatggat
     7501 tatctgtttt tgtcgataat tacggcgcta tatgtgccgt tctttttgca gtgatcacag
     7561 gtttaatatc atggtctctg ccaaactgga aaagccctga cagtgttcgt acttttgccg
     7621 acaagataat gccatggagt atttatcagg atatacaggg agcaacattt ctcctgaata
     7681 tggctgcact tctgaaagcc aaaatgacga cgctcaactc gctaaacatt cttcaggagt
     7741 ttgcatcacc atggttaagc acacgtcttg acagcattat ttaccgcgtc cgccaggggg
     7801 atcatcttgg actggctttg cgtcagtgcg gttatcagtt cccatccagg gaagcagcca
     7861 acttcctgtc tttattacag ggagatggcg caacagagct aatcagcaat tatggtcaac
     7921 gatggcttag tcaaacactt caacgtgtaa agaaacgggc aaacgtgatt cgtttaatta
     7981 tgctgatatt tcttgtcatg tctctcatgt tactggtttt tgccattatg gatattcagt
     8041 ctattagtga caacagcatg ggtaactt
//
LOCUS       DAPGRN010000168         8064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPGRN010000168 DAPGRN010000000
VERSION     DAPGRN010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.170"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1221
                     /locus_tag="RHW33_004294"
     CDS             55..1221
                     /locus_tag="RHW33_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDV1647738.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGIVATLKVGDGEKSIGLRADMDALPIYENSGKLWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDHFPCDVIFGM
                     HNMPGLPVGKFYFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1264..2580
                     /locus_tag="RHW33_004295"
     CDS             1264..2580
                     /locus_tag="RHW33_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV1647739.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADKNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLICSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTMLSMLALLYVKEPAKELQ"
     gene            2684..3334
                     /gene="fabR"
                     /locus_tag="RHW33_004296"
     CDS             2684..3334
                     /gene="fabR"
                     /locus_tag="RHW33_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV1647740.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3334..3693
                     /locus_tag="RHW33_004297"
     CDS             3334..3693
                     /locus_tag="RHW33_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV1647741.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3733..4833)
                     /gene="trmA"
                     /locus_tag="RHW33_004298"
     CDS             complement(3733..4833)
                     /gene="trmA"
                     /locus_tag="RHW33_004298"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV1647742.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4947..5137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5202..7085
                     /gene="btuB"
                     /locus_tag="RHW33_004299"
     CDS             5202..7085
                     /gene="btuB"
                     /locus_tag="RHW33_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV1647743.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPVNTVLA
                     PTDIVTREDIQRWQSKDLNDVMSHLPGVNISQTGGMGTSSSLYVRGTESRHVLVLIDG
                     VPMARAGIDNAIDISQFPVSLVQRVEYIRGPRSAVYGSGAIGGVVNIITMSNDEKAQI
                     NAGMGTNGYQAYDGVINKRFGDTMVTAAGAYQTTKGFNIQPGSPYSGDSDRDGYRNKL
                     FWGGLQHKFNDNFSGFFRGYSYTANNDYDQGSMYGSATGNEEAQNYNQSWDTGLRYNS
                     GIYSSQLIANYQRLKNYNYTNNLGRYAGDATLDDMEQRYIQWGNSIEVGHGAISGGID
                     WKQEKLTSSSITKSDDYERDTTGLYLTGQQQISNVTLEASGREDHDEQFGWHGTWQTA
                     AGWEFIDGYQATLSYGTSFLSPSLGQQYGAARFASIYGPGIAANPNLKPEESKQWEIG
                     IEGVTGTLDWRLSGYRYEIQNLIDYKSINNVNQYINVKSATIKGLEWTGNITTGPVEH
                     RLTLQYVDPRDDETDKVLYRRAKQQVKYELTGQAYGFEWDVSYQYIGQRYDNAYDETS
                     GSSHTVKLGGVSLWDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGS
                     YTF"
     gene            7030..7887
                     /gene="murI"
                     /locus_tag="RHW33_004300"
     CDS             7030..7887
                     /gene="murI"
                     /locus_tag="RHW33_004300"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV1647744.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1887 a         1870 c         2169 g         2138 t
ORIGIN      
        1 ccatcaataa aatccgttaa ttagcctgtt tataagtgaa taaaggaata gccgatgatc
       61 gaacactata ttcgagggtt tgaggaagaa ctgcgtgaga tccgccatca gatccacgaa
      121 aacccggagc tgggattaca agaatttaaa accagtgctt tggttgcgga aaagctgcgt
      181 cagtggggct atgaagtaga gcaaggactg gcgacgacgg ggatagtggc cacgttaaaa
      241 gtgggcgacg gagagaaaag tattggcctg cgggcggata tggatgcgct gccgatttat
      301 gaaaatagtg gcaagctctg ggccagtaaa catcctggtt taatgcacgc ctgcgggcat
      361 gacgggcata ccaccatact gctgggggcc gcgcgttatt ttgccgaaac ccgccgtttt
      421 aacggcacgt tacgtcttat cttccagcct gcggaagaga tgattaatgg cggtgagatc
      481 atggttaaag aggggctttt tgatcatttc ccctgcgatg tcattttcgg catgcataac
      541 atgcccggtc tgccggtggg taagttttat ttccagccag gggcactgat ggcgtcaatg
      601 gatcagttcc atattacagt tcgtggttgt ggcggacacg gtgcgatccc gcacaaagcc
      661 atcgatccgg tgctggtcgc ggcacatatc accaccgcat tacaaagcat tgtgtcgcgc
      721 aatgtcgatc cgctggaagc ggcggtaatt accgtcggca gcattgttgc gggtgaggcg
      781 gctaacgtca ttcctgacag tgctgagatg aaaatcagcg tccgctctct tagccgcgat
      841 acccgacaac ttcttctgac ccgtattcct gctctggcac aagcccaggc tgccagcttt
      901 ggcgctacgg ctgaggttac gcatgttaac ggtacaccag tattagtaaa tgacgaggag
      961 atggcgcgct tcgcctggca ggtggcgtgt aaaacgtttg gtgaagatcg ggccgagttt
     1021 ggcatcaagc ctctgatggg cagtgaagat ttctctttca tgctggaagc ccaacccaaa
     1081 ggcggtttcc tgttattcgg taatggcgat gtcggggaag gttcctgcat ggtgcataac
     1141 ccgggttacg acttcaacga tgcaagtctg gtcccggcaa gcagctactg gggcgcactg
     1201 gtagaagcct ggctgcaata aataaaacaa cacacaacaa cactttttgg caaggaacat
     1261 ggaatgacgg cgatcgtaaa tgaaaccgtg agtgtgaaga aaaagcccag cggcaggcgg
     1321 gtgatttttg cctcggcgtt tggcaatgcg ctcgaatttt ttgattttgg cgtttacaac
     1381 ttttttgtgg tctatatcag cacgctattt tttccgccca gtgcagataa aaacgtcgcg
     1441 ctactgctgg cgtttgccac tttcggcgtg agctttttta tgcgcccgct cggcggcatt
     1501 attgtggggg cctgggccga tcgtttcgga cgcaaacccg cgatggtgtt caccattgcg
     1561 ctgatgagtc ttggtacact gatgatcggc atcgcaccga cgtatgaaac ggcgggctat
     1621 tggggaaccg cgacgctggt gctagcgcgt ctaattcagg gcgttgcggc aggaggcgaa
     1681 gtcggtgcct caatgtcttt gttggttgaa tcagctccgg caaatcgtcg cggtttttac
     1741 agcagttggt cactggcgac tcaaggcctg gcaacaactt ttggaggtgt tgtcgcttta
     1801 ggtttaagtg catggctgcc ctttgcgaca ggttcagaaa ccgtaatggc tgaatggggc
     1861 tggcgtgtgc cattttttat tggcgtgtta ttagcaccta tcggttgctg gttgcgtctg
     1921 agcctggaaa atgacgtacc tgagcctgtg cgcaataaaa aagctgctac cagcgaaagc
     1981 gcgttttctc tgctcctgca acataaggcg actattgtta atggcgtttt actcgctatt
     2041 ggtagcacgg tcgcaaccta tatctccctc ttttattacg gcacctgggc ggcgaagtat
     2101 ctggcgatgc ctcaacatta ttctcatgca gctatgttac tggcgggcgt tattacgttt
     2161 gtgggtgcgc tgctggtggg gatgttgtgc gattcggtcg ggcgtaaaaa gctgatttta
     2221 atctcccgtg tgatggtgct gatctgtagc tggccgtcat tctggctgtt ggtgaattac
     2281 ccaagccctg gcatgttgct gacggtggtt ttcgtgatgg tcagctttac cacgcttggc
     2341 ggtgtgccag tgatgttgtt gatctctgaa ctgttgccga aacggattcg ggcattgggt
     2401 tttgcgctgg tttatagcat tggtgtggca atctttggcg gttttgctca atatttcgct
     2461 acgcaatcca tcgtgttgct ggatagtttg acggctccgg cgtggtatct gggtggaggc
     2521 acgatgctgt cgatgctggc gttgttgtac gtaaaagaac cggccaaaga attgcagtaa
     2581 atatgtttta ttgcgttacc gttcattcac aatactggag caatccagta tgttcattct
     2641 ctggtatagt gccagcagta cttttggcaa ggattcagac atcgtgatgg gcgtaagagc
     2701 gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagca gcatttagcc aattaagtgc
     2761 tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc
     2821 cacctctttt tatcggcatt tccgcgacgt agacgaactg ggtctgacca tggttgatga
     2881 gagcggttta atgctacgcc aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg
     2941 gagtgtgatc cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc
     3001 cttccggtta ttattgcggg agcgctccgg cacctccgct gcgtttcgtg ccgccgttgc
     3061 gcgtgaaatt cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat
     3121 gccgcgtgcg tttactgaag cgcaagccga agcaatggtg acaattgtct tcagtgcggg
     3181 tgccgaggcg ttggacgtcg gcgttgaaca acgtcggcaa ttagaagagc gactggtact
     3241 gcaactgcga atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac
     3301 cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt
     3361 acgctgctgc tggcgttagt tgctggctta tcgattaatg gtactttcgc agcgctgttt
     3421 agctccattg tgccattttc tgtattcccg attatttccc tggtgctgac ggtttactgc
     3481 ctgcatcaac gttatcttaa tcgcaccatg ccggtaggct tgccgggtct ggcagctgcc
     3541 tgttttattc tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc
     3601 ggctctaact tcttcccggc agtactctcc gtcattatgg tgttctggat tggcgcgaag
     3661 atgcgtaacc gtaagcagga agttgctgag taatcggtat catgccggat gctggcgcat
     3721 ccggcatggg ttttacttcg cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg
     3781 gaactgatca aacagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt
     3841 tttgcataac gtttccggat tgcaggagat atacagaata cgtggatacg cctgcaccat
     3901 tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg
     3961 ataactcttt aagtcgatcc cttgcaggcg gttaaactcg cgcacgccat tcatcgcctg
     4021 agtaaattct tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagcggcaat
     4081 attgtattgc gcggcagcga ctgacggctt ggcgatttcg gtggctaata cccgatcaaa
     4141 attgcgcgcc agggctaatg agaagttacc gttgccgcag tacagctcca gtaaatcgcc
     4201 ttttgagcct ttggttacgt ccagtgccca ttccagcatc tgaatattca tcgccgcgtt
     4261 cggctgggta aagctgtttt ctacctgacg gtagatcatc tctttccctg cgaccggcag
     4321 acgttcgtcg atgtaatctt gatccagcgc gattttggtt ttcgttgccc gaccaatcag
     4381 atgcacattc agattctgcg cgcgcagtgc atcgcgcagt gcttccgctt cctgacgcca
     4441 ctcatcatcc agctttttat ggtacagcaa ggaaaccacc gcctgattac tcagcgtggt
     4501 gaggtaatca atctggaaca acttgtggcg cagaatggga ttattacgca cacccgcaat
     4561 catcgccgtc atcaactggt tgatgagttc actggcggcg gggaagctat ccacgcggat
     4621 acggcttttg gtttgttgat cgaaaatgat gtgatacagg tcatcgccat cgtgccagat
     4681 gcggaactcc gcacgcatcc ggtaatgact gaccggcgag cgaaacactt ccggaaccag
     4741 gtcagaaaac ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc
     4801 atactgttct gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc
     4861 ggcgattgta gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct
     4921 acgtctggac agtaacttgt tacaacctgt agcatccact tgccggtcct gtgagttaat
     4981 agggaatcca gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc
     5041 gttatgcgga cactgccatt cggtgggaag tcatcatctc ttagtatctt agatacccct
     5101 ccaagcccga agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga
     5161 tgaaacctgc ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc
     5221 tgctgacggc gtgttctgtc acagcctttt ccgcttgggc acaggatacc agcccggata
     5281 ctctcgtcgt tactgctaac cgttttcagc agccagtaaa tactgtcctt gcacctactg
     5341 atattgttac ccgtgaggat attcaacgct ggcagtcgaa agacctcaac gatgttatga
     5401 gtcatctccc cggtgtaaat atctctcaaa ctggtggaat ggggacgagt tcttctcttt
     5461 atgttcgtgg cactgaatcg cgccatgttt tagtgttgat tgacggtgtg ccgatggctc
     5521 gtgccggtat cgacaatgca attgatatta gccagttccc ggtatcactt gtacagcgtg
     5581 ttgaatatat tcgtgggccg cgttctgccg tttatggttc tggtgccatc ggaggtgtgg
     5641 tgaacattat tacaatgagt aatgatgaga aagctcagat caacgcagga atgggtacaa
     5701 atggttatca agcctatgat ggggtaataa ataaacgttt tggcgatacg atggtaacag
     5761 cagcaggtgc ttaccagacg actaaaggtt ttaacattca gcctggctca ccatacagtg
     5821 gtgatagcga tcgcgatgga tatcgcaaca aacttttctg gggggggctg cagcataagt
     5881 ttaacgataa cttctcgggg ttcttccgtg gttacagcta cacagctaat aatgattacg
     5941 accagggctc gatgtatggt tctgctactg gcaacgaaga agctcagaat tacaaccagt
     6001 cctgggatac tggcctacgc tataactccg gcatctattc gtctcagtta attgctaact
     6061 atcaacgact caagaattat aactacacaa ataatcttgg tcgttatgcg ggagatgcca
     6121 ccctggatga catggaacag cgttatattc agtggggtaa tagcattgag gtagggcatg
     6181 gtgcaattag cggcgggatc gattggaaac aggaaaaact gacgtccagc agcattacaa
     6241 aatcagatga ttacgaacgc gataccaccg ggttatacct gactggacag caacagattt
     6301 ccaatgtgac tttggaagct tctggccgag aagatcatga tgaacagttt ggctggcatg
     6361 gtacctggca gacagctgct ggctgggagt ttattgatgg ctaccaggca acactttctt
     6421 acggtaccag ttttctttcg ccttctcttg gacagcaata cggggctgct cgctttgctt
     6481 caatttatgg tcctggtatt gcagccaacc caaatttgaa gccagaagaa tctaaacagt
     6541 gggagattgg aattgagggg gtaactggga ctctggattg gcggctctca ggatatcgtt
     6601 atgaaattca gaacctgatt gattataaaa gcataaataa tgtcaaccag tacattaatg
     6661 tgaaatcagc cacgattaaa gggctggaat ggacgggaaa tattacaacc ggtcctgttg
     6721 aacatcgctt gacgttacaa tatgttgacc cgcgcgatga tgaaacagat aaagtgctgt
     6781 accgtcgggc aaaacagcag gttaagtacg agttgactgg acaagcttat ggtttcgaat
     6841 gggacgtttc ttaccaatat ataggtcaac gatacgacaa cgcttatgac gagacctctg
     6901 gctcatcaca tacagttaaa ttaggcggtg tgagcttgtg ggatcttgcg gttgcatatc
     6961 cggtcacctc tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg
     7021 agacagtcta tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct
     7081 tctgaaccac gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat
     7141 gacgagatcc ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct
     7201 ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc
     7261 gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc
     7321 gtttcacttc ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg
     7381 attaaacctg ctgcacgcct gacggcaaat ggcattgtcg gattactggc aacccgcgga
     7441 acagttaaac gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata
     7501 gaaatgctgg gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat
     7561 gtttctctgg atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca
     7621 gataccgttg tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg
     7681 ctgccagagg gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg
     7741 ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg
     7801 gcaatgacgc cagaagctga acaattattg cccgtcttac agcgttacgg cttcgaaacg
     7861 ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt tgcgcggtcg gaaaattatt
     7921 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
     7981 caacggcaaa cacgccgccg ggtcagcggg gttctcctga gaattccggc agagaaagca
     8041 aaaataaatg cttgactctg tagc
//
LOCUS       DAPGRN010000169         8054 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPGRN010000169 DAPGRN010000000
VERSION     DAPGRN010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.171"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..4175
                     /locus_tag="RHW33_004301"
                     /pseudo
     CDS             152..4175
                     /locus_tag="RHW33_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            4172..5572
                     /locus_tag="RHW33_004302"
     CDS             4172..5572
                     /locus_tag="RHW33_004302"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1647745.1"
                     /translation="MNSEGGKPGNVLTVNGNYTGNNGLITFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIEIIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPPDTPAPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQDSASSMWMRHVGGHERSSAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANMT
                     GAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTCSGSEGTLNTWY
                     VQPQAQITWMGVKDSDHARKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQ
                     PYIEANWINNSEVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGD
                     KGYSDTQGMLGVKYSW"
     gene            5783..6049
                     /gene="hipB"
                     /locus_tag="RHW33_004303"
     CDS             5783..6049
                     /gene="hipB"
                     /locus_tag="RHW33_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV1647746.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            6049..7371
                     /gene="hipA"
                     /locus_tag="RHW33_004304"
     CDS             6049..7371
                     /gene="hipA"
                     /locus_tag="RHW33_004304"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV1647747.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPLEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK"
     gene            7642..7911
                     /locus_tag="RHW33_004305"
                     /pseudo
     CDS             7642..7911
                     /locus_tag="RHW33_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2194 a         1727 c         2199 g         1934 t
ORIGIN      
        1 attcgaaagc attagcaaat tagccaaatt gacactttcc cacctccaac taacactcaa
       61 tacagacctc tgacaattta ataaatttca tttttaatgt ctgacgactc atctctttcg
      121 tggctgtatt gtttgtatat ggagcgttta tatgaataga atctatcgcg tgatatggaa
      181 ttgcactcta caggtatttc aggcctgctc ggaattaact cgcagggtag gtaaaacatc
      241 aacggttaat ttgcgaaaat cctctggact gacaacgaaa ttcagtagat tgacgctggg
      301 tgttttgctg gcactaagcg gttcagtgtc tggtgcgagt ctggaagctg ataatggtca
      361 gattaccaat attgatactg atgttgctta tgatgcctac ctggttggct ggtatggcac
      421 tggagtgctt aatattttgg ctggcggtaa tgcctcctta accactatta ctaccagcgt
      481 cattggcggt aatgaggatt cggagggtac cgttaatgtt ttgggtggca cctggcgatt
      541 gtatgatagc ggaaataatg caaggtcttt aaatgtgggt caatccggaa cggggacgct
      601 gaatattaaa cagaagggtc acgtcgatgg aggctattta agattaggta ctcaagctgc
      661 aggtgtcggg acggttaatg ttgagggaga ggactcggtt ttgacgaccg aattattcga
      721 aatagggagt tacggtacgg gctcattaaa tattacggat aagggttacg tcacgagttc
      781 aatagtcgcc attttaggat atcaagcgaa cagtaatggt aaggttgtcg ttgaaaaggg
      841 tggcgagtgg ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg
      901 aactggggag gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg
      961 tggcaatgcc accggtgtcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt
     1021 acgcagactc tataatggat atttcggtaa tggcgcagtc aatatttcca ataatggact
     1081 aattaataac aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa
     1141 cgtgaccgat aaagggcatt ggaatttcct cggaacgggc gaagctttcc gctatatcta
     1201 tatcggtgat gctggcgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg
     1261 aattatcact gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa
     1321 ctccgttatc actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat
     1381 cagtaatgag gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaccgg
     1441 cgtcgggaag gttagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac
     1501 caccattggt gttgcgggcg tcggaaacct caatattagc gatggcggta agttcgtatc
     1561 gcaaaatatt acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga
     1621 tgcgacatcg tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt
     1681 aaatgtcagt aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc
     1741 ctccggtaag gggatagtta atatttcaac ggatagtctc tggaatttaa agacgtcttc
     1801 tactaacgcc caattgctac atgtcggtgt attaggcacg ggtgaactga atattaccac
     1861 cggaggtata gttaaagcgc gtgatacaca gatagctctc aatgacaaaa gtaagggcga
     1921 cgtgagggtg gatgggcaga actctcttct tgaaacattc aatatgtacg tagggacatc
     1981 tggtacgggt acgttaaccc tgacgaatag cggcacgctg aatgtcgaag gtggagaagt
     2041 ttacttaggt gtttttgaac ctgctgtagg aacgctaaac attggtgcag ctcacggtga
     2101 ggcggcggca gatgccggat ttatcaccaa cgcgacgaaa gtagagtttg gctctggcga
     2161 aggtgttttt gtctttaatc atactaataa cagtgatgcc ggctaccagg tcgatatgct
     2221 gattacaggt gacgataaag acggaaaagt gatccatgat gcaggccata cggtgttcaa
     2281 tgcagggaat acttatagcg gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc
     2341 tcatacggca gatggggtaa cgggcatggg ttcgagtgaa gtaaccattg caagccccgg
     2401 tacgctcgac attctcgcat caacgaacag tgcaggagat tacacgctga ccaatgcgct
     2461 caaaggcgat ggcttgatgc gagtgcagct gtcatcctcc gacaagatgt ttggctttac
     2521 ccatgcaaca gggactgaat tcgccggtgt tgcccaactg aaagacagta ccttcactct
     2581 ggaacgcgac aacaccgctg cgcttactca cgcgatgttg cagtctgaca gtgaaaatac
     2641 cacatcggta aaagttggag agcaatccat tggtggactg gccatgaatg gaggtacgct
     2701 cattttcgat acggatattc ctgctgcgac gctcgcggag ggatatatca gcgtcgatac
     2761 gctggttgtc ggcgcgggtg actacacctg gaaaggccgt aactatcagg taaacgggac
     2821 gggcgacgtg cttatcgacg tgcctaaacc gtggaatgat cccatggcga ataaccctct
     2881 gacgacgctc aatttgctgg aacacgacga tagccatgtc ggcgttcaac tggtgaaggc
     2941 gcaaacggtt attgggtcgg gtggctcatt aacgttacgt gatttacagg gcgacgaggt
     3001 ggaagcggac aaaacgttac acattgcgca aaacggaacg gtggtcgccg agggtgatta
     3061 tggattccgc ctcacgaccg caccaggtga tggtttgtac gttaactatg ggctgaaagc
     3121 gctgaacatc catggagggc aaaagctgac gttagccgaa catggcggag cctatggcgc
     3181 aacggccgat atgtcggcaa aaatcggtgg tgaaggggat ctggcaatca atacggtgcg
     3241 acaggtttcg ctttccaacg gtcagaacga ctatcagggg gcaacctacg ttcagatggg
     3301 gacattacgt accgatgcgg atggcgcgct gggcaatacc cgggaactga acatcagcaa
     3361 cgcggccatc gtcgatctta atggatcgac gcagacggta gagacattca tcgggcagat
     3421 gggttcgact gttttgttca aagaagggtc gctgacggta aataaaggtg ggatcagtca
     3481 gggtgaactg acaggtggcg gaaacctgaa tgttacaggg ggaacgctgg ctatcgaggg
     3541 gcttaatgca cgctacaatg cgttaaccag cattagccca aatgcggaag tcagcctcga
     3601 taatacgcag gggttaggca gaggaaatat tgccaatgac ggtctgttaa cgctaaaaaa
     3661 cgtgactggc gaactgcgta atagcataag cgggaagggt atcgtgagcg caaccgccag
     3721 gacagatgta gagctggatg gcgataatag ccgctttgtg gggcaattca acattgatac
     3781 aggcagcgcg ctcagcgtca acgagcagaa aaacctgggt gatgcttccg ttatcaataa
     3841 tggcctgctc accatctcca ctgagcgtag ctgggcgatg acgcacagta tcagcggtag
     3901 cggtgatttg acaaaactgg gtaccgggat cctgactctt aacaacgatt cctcggcgta
     3961 tcagggtacg acggatatcg tgggggggga aattgctttc ggttccgact ctgccattaa
     4021 tacggcaagt cagcacatta atatccataa cagcggtgtg atgtcgggaa atgtcaccac
     4081 tgcaggtgat gtgaacgtta tgtctggggg gacactgcgt gtcgccaaaa ccacaatcgg
     4141 cggcaacctg gagaatggcg gcacggttca aatgaacagt gaaggaggaa aaccggggaa
     4201 tgtactgacc gttaacggca actataccgg aaacaatggc ctgataacgt tcaacgcgac
     4261 gctgggcggc gataattcac ccaccgataa gatgaacgtg aaaggcgata cccaagggaa
     4321 cactcgcgtt cgggttgata acattggcgg cgtcggtgcg caaacggtca acggtattga
     4381 aatcattgag gttggcggta attctgcagg taacttcgcg ctgaccaccg gaactgtcga
     4441 agctggggct tacgtctaca cgctggctaa agggaagggg aatgacgaga aaaactggta
     4501 tctgaccagt aaatgggacg gcgtaacgcc accggataca cccgctccca ttaataatcc
     4561 ccctgttgtg gatccggaag gcccatcagt ttatcgcccg gaggccggaa gctatatcag
     4621 caacattgcc gcagccaact cgctgtttag ccatcgctta cacgaccgtc tgggtgagcc
     4681 gcaatataca gattcactgc attctcagga ttcagcaagc agtatgtgga tgcgtcatgt
     4741 cggggggcac gaacgttcca gtgccggaga cggccagcta aatactcagg ctaaccgcta
     4801 tgtattgcag ctaggcggcg atttggcgca gtggagtagc aacgcgcagg atcgctggca
     4861 tcttggcgtg atggcaggct acgccaatca gcacagtaat actcagagta atcgtgtggg
     4921 ttataaatcg gatgggcgca tcagcggtta cagcgctggg ctgtacgcga cctggtatca
     4981 gaacgatgcg aatatgaccg gcgcttatgt tgacagctgg gcgctgtata actggtttga
     5041 taacagcgtc agttccgata accgttctgc tgacgactat gattctcgcg gtgtgacggc
     5101 ctctgttgag ggtgggtata cctttgaagc gggaacatgt agcggcagcg aagggacgct
     5161 gaatacctgg tacgtccagc cacaggcgca aatcacctgg atgggtgtga aagattctga
     5221 ccatgcccgg aaagacggaa cgcgcattga aacggaaggc gacggaaacg tgcaaacgcg
     5281 acttggggtg aaaacctacc tgaatagcca tcaccagcgt gacgatggta aacagcgtga
     5341 gttccagcct tacattgaag cgaactggat caataatagc gaagtctacg ccgtgaagat
     5401 gaatggtcaa accgtaagcc gtgatggtgc gcgtaatctc ggtgaagtac gtaccggggt
     5461 tgaggcgaaa gtaaataaca accttagcct gtgggggaat gtcggtgtgc aactaggtga
     5521 taaaggctat agcgatactc agggcatgct gggagtgaaa tatagctggt aaaccgtata
     5581 agccgcatgt cgagatggca tgcggcttaa tattgccaac ttcaaaaggc ttttaaagcc
     5641 tcccttttat ccgcgatcgc ggatatctca gcgtttatcc cgtacagcgg ataagatgtg
     5701 tttccagatt gactttatcc tcactaaagg ataaaactga taatatcccc ttaagcggat
     5761 aaatttgctg tggacgtatg acatgatgag ctttcagaag atctatagcc caacgcaatt
     5821 agcgaatgca atgaaactgg ttcgccagca aaatggctgg acgcagagcg agctggcgaa
     5881 aaaaatcggc attaagcagg cgacgatttc caatttcgaa aacaaccctg acaatacctc
     5941 gctcacgaca ttttttaaga ttttacagtc gcttgaactc tcaatgacgc tatgcgacac
     6001 gaaaaatgcc tcgccagaat caacagaaca gcaagatctg gagtggtaat gcccaaacta
     6061 gtcacttgga tgaacaacca gcgggtaggc gagttaacga agttagccaa cggcgcgcac
     6121 acctttaagt atgcaccgga gtggttagcc agccgttatg ccagaccgtt gtcactttcg
     6181 ctgccattgc agagggggaa tatcacctct gatgccgtat ttaacttctt cgataacttg
     6241 ttacccgata gcccgattgt acgtgaccgg atcgttaaac gttatcatgc caaatccaga
     6301 caaccgtttg atttattgtc agaaataggg cgagacagcg ttggtgccgt aacgttatta
     6361 cccgaaaatg aaaccataac gcgcccgata atggcatggg aacagcttac tgaagccaga
     6421 cttgaagaag tattaacggc ttataaagca gatatcccgc taggcatgat tagagaagaa
     6481 aatgactttc gcatctcggt tgctggcgca caggagaaga cagcgctgct cagaataggc
     6541 aatgactggt gcatcccgaa aggaataacg ccgacgacgc acatcattaa attaccgatt
     6601 ggggaaatca ggcagcccaa tgcgacgctc gatctcagcc aaagcgttga taatgagtat
     6661 tactgtctgc tgctggcgaa agaacttggg ttgaatgttc cggacgcaga aatcattaaa
     6721 gcgggaaatg tgcgcgcgtt agcggtcgaa cgttttgaca ggcgttggaa tactgagcga
     6781 acggttttac ttcgcttgcc actggaggat atgtgtcaga cattcggttt accttcatcg
     6841 gtgaaatatg aatcagatgg aggcccaggc atcgcgcgga tcatggcttt tttgatgggg
     6901 tccagcgagg cgctgaaaga tcggtatgat tttatgaaat tccaggtctt tcagtggttg
     6961 attggcgcaa cggatggcca tgcaaaaaac ttctccgtat ttattcaggc tggcggcagt
     7021 tatcgactca cgccatttta tgacatcatt tcagcatttc cggtccttgg cggtacgggg
     7081 atacacatca gcgatctcaa actggcaatg ggacttaacg catccaaagg caaaaaaacg
     7141 gcaatcgata aaatttatcc gcgacatttt ttggcgacag caaaggtgct gagattcccg
     7201 gaagtgcaga tgcatgaaat cctgagtgac tttgccagaa tgattccggc agcactggat
     7261 aacgtgaaga cttcattacc gacagatttt ccagagaacg tggtgacggc agtggaaacc
     7321 aatgtgttga ggttgcacgg tcggttaagc cgagaatacg gtattaagta agagtaagat
     7381 atggggtttg gtcgttgtta atgagcagga caatcatgac cgccaacgac tagattctcc
     7441 cttggtttat gttagtgcgc ataatgtata ttatgttaaa tcgcatctga gtgatacgaa
     7501 gtgattttcc ggtagtcgtt attcgttcag ggttccagaa cttaagcctc tcctctttca
     7561 taattggcct gtgcatgccg gatataggct ggctggtatg cagatttgcc gtttgccgca
     7621 tcttcccagc gttgaagact caagctggta aaccgcgtgc aaatgagcac gccctctttc
     7681 catcaacact tcaggcagct tgcgggaatg agtccactcc ggtatcagaa atggctacgt
     7741 ctcaatgaag tcaggcgaac gatgctgaat gaacattacg atgtcaccac agcagcctat
     7801 gctgtcggtt acgaaagctt atcccatttc agccgggaat atttgcggat gtttggagag
     7861 tcacctaaga gagatattac caggttaaga aagtctgtcg gtcagcttta ataaaccctg
     7921 cacttatctg tttagagcaa ttcggtgtta gttacatcag gcaaacatta actatagcta
     7981 atgatttata gccatataaa ccattagtag cccttgacct ttcctgacac ttcccttgtt
     8041 cgcaataata ttct
//
LOCUS       DAPGRN010000170         8046 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPGRN010000170 DAPGRN010000000
VERSION     DAPGRN010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.172"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(217..1410)
                     /gene="tyrB"
                     /locus_tag="RHW33_004306"
     CDS             complement(217..1410)
                     /gene="tyrB"
                     /locus_tag="RHW33_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV1647748.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAVPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1663..2742)
                     /gene="alr"
                     /locus_tag="RHW33_004307"
     CDS             complement(1663..2742)
                     /gene="alr"
                     /locus_tag="RHW33_004307"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV1647749.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEAHLEEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2795..4210)
                     /gene="dnaB"
                     /locus_tag="RHW33_004308"
     CDS             complement(2795..4210)
                     /gene="dnaB"
                     /locus_tag="RHW33_004308"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV1647750.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4293..5276
                     /locus_tag="RHW33_004309"
     CDS             4293..5276
                     /locus_tag="RHW33_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV1647751.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5442..5684)
                     /gene="pspG"
                     /locus_tag="RHW33_004310"
     CDS             complement(5442..5684)
                     /gene="pspG"
                     /locus_tag="RHW33_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV1647752.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWHY"
     gene            complement(5818..6855)
                     /gene="dusA"
                     /locus_tag="RHW33_004311"
     CDS             complement(5818..6855)
                     /gene="dusA"
                     /locus_tag="RHW33_004311"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV1647753.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7256..7714)
                     /gene="tnpA"
                     /locus_tag="RHW33_004312"
     CDS             complement(7256..7714)
                     /gene="tnpA"
                     /locus_tag="RHW33_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDV1647754.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMLYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
BASE COUNT         2045 a         2120 c         1927 g         1954 t
ORIGIN      
        1 taatggttag atgtgcagaa attattactc cacacgaaaa aaacagggaa aggaattcca
       61 gataattagg ataatattaa caataaaaga actaacaacg tttattatct ctcacgtaga
      121 acgatggtat caaaaaaatc atctataaag aaagagtgaa agcgacgaca gtttaatttc
      181 actgcaggct gggtaattcc agcctgcttt cctgcattac atcaccgcag caaacgcctt
      241 tgccacacgt tgtacatttg ccgtatttaa cccggcgaca cacatgcgac cgctggcgat
      301 gagatagaca ccaaattctt cacgtagtcg gtcaacctga gcggcactta aaccggtata
      361 actgaacatg ccgcgctgat taagcagata atcgaaattg cgttctggca tctctgtgct
      421 caataccttc accagttcct gacgcattgc cagaatgcga gtacgcatct cttctacttc
      481 cgccagccag ctggctttca atgcctcgtc attcagcacc gcagccacca cctgcgcacc
      541 aaaattcggc gggctggagt agttgcggcg aactgttgct ttcaattgcc ccagtacgcg
      601 gcctgcggct tcggcatctt cacacagaac agaaagtccg ccaacgcgct cgccgtaaag
      661 ggagaaaatt ttcgagaacg aattgctcac cagagcgggt aatccagcgc tggcaatggc
      721 gcgaatggcg taggcatcct cttccatacc ggcaccaaat ccttgatagg caatatcaag
      781 gaatgggata agctcgcggg ctttgagaat ttcaatcacc gcatcccact ggtcattagt
      841 gagatcggca cccgttgggt tgtggcaaca tggatgcagc aacacaatac tgcgggcagg
      901 taatgttttc agcgtcgcca acaggtcatt aaagcgcacg ccgttagtcg cttcgtcata
      961 ccaggggtaa gtactcactt cgaatccagc cccggcgaat attgctacgt ggttttccca
     1021 ggtaggatcg ctgacccaga cgcctgattc cgggaagtag cgtttcagga aatccgcgcc
     1081 cactttcaat gcccctgagc cgccaagggt ttgaatggtt gctacgcgct gttgttgcag
     1141 taccggatgg tcggcaccaa acagcagcgg cgcaatggca tggcgatagc tgttaagccc
     1201 ttccatcggt aaataaagcg aagcgccatg aggcaccgca ttcaggcgcg cttctgcatc
     1261 agccacggct ttcaattgtg gaataattcc gtcttcgttg tagtacagac cgatacttaa
     1321 attcactttg tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc
     1381 gccagcgtag gcgtcaactt tttgaaacac gcgatggttc tccaggttta cgggcaggtg
     1441 gttaaaacac aataaaccgg aagaaggcga agatcgagtg gatgttcagg ggcgaacggc
     1501 aattagcaac agagtgagac tcatgacaaa cgtacatccg ccagaggcac gacctttata
     1561 agagcatgaa agaatatcaa cttattgaat tggtaggatt ttattggccg gataaggcat
     1621 tcacgccgca tccggcacag acaatcaaat attacagaac gattaatcca cgtatttcat
     1681 cgcgaccctt gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg tcatttcagc
     1741 gatacgttct acgggcaaac cttcgcccca caaaatgacc ggatccccgg ctttgtcctg
     1801 cgcctgtgga cctaagtcta cgcagatcat atccatcgcg acgcgcccga caatcggcac
     1861 ttcgcgaccg ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc
     1921 atagcccatc gcgactacgc caagacgagt atcacgttcg cttacccagg ttccaccata
     1981 accgacaggc tctccggctt tatgctcacg cacggcaatc aggctggagg ttagcgacat
     2041 cactggctga cagccaaaat cggcaccggt agagcgatct tccagcggag atacgccgta
     2101 aagaatgatg cccgggcgca cccagtcaaa atgcgactgt ggccagagta aaatgccacc
     2161 cgacgcggca atggaacgtt gaccaggttt gccttcgcaa aaggtattaa agatagcgag
     2221 ttgtttctcg gttgcgccgc attttggttc atccgcgcgc gcaaaatggc tgacgatatt
     2281 caccggctga cgaacgtttt tgcactgggt caggcgatga taaaacgcct cagcctgttc
     2341 cggccttacg cccagacggt gcataccggt atcgagtttc atccagacgg taaccggctc
     2401 ttccagatgc gcctcttcca gcgcagccag ctgttcttcg ttatgtacgg cggtatgaaa
     2461 atgttgcgca gaaatcgtcg gcagatctct ggcatcaaaa aagccttcga gtaacagtac
     2521 aggtttggtg attccccccg cacgcagtcg cagagcttct tcgagacggg ctacgccaaa
     2581 ggcgtcagca tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc
     2641 tttcaccacc gcaaccattt tactggcagg tgccagttca cgaagacgtt gcaggttgtg
     2701 tcgcagagcg cggcggttaa tcacaacagt tgccgcttgc atttgtgttc cttgataagt
     2761 gtttgcttta attacctaat tcataaaata attattattc gtcgtcgtac tgcggccccg
     2821 catagttgtc gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc
     2881 cgttacgttg tttaccgata ataatttccg cgatgccttt taaatcactg ttttcgtgat
     2941 acacctcatc acgatagata aacatgatca agtccgcatc ctgctcgata gagccagatt
     3001 cacgcaggtc ggagttgacc gggcgtttgt cggcacgttg ttccagagaa cggttcaact
     3061 gggacagcgc caccactggc acgttcagtt ctttcgccag tgctttcagc gagcgggaga
     3121 tttctgcaat ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt
     3181 agtcgatcat gataagcccg atgccgccgt gttcacgggc aatacggcgt gcgcgggaac
     3241 gcacttccgt tggcgtcagg ccggaggagt catcgatata gatattgcgt ttttcgagca
     3301 aaatacccat ggtgccggaa atgcgcgccc agtcttcatc atcgagctgc ccggtacgga
     3361 ttttagtctg gtcaacgcgc gacagcgacg ccagagaacg catcatgatc tgttctgatg
     3421 gcatctccag cgagaagata agtaccggtt tatcctgcaa catcgccgcg ttttcgacga
     3481 ggttcatcgc aaatgtcgtt ttacccatcg acggacgcgc ggcgacgatg atcaaatccg
     3541 acggctgcaa gccagcggtt tttttgttga gatcgtcata accggtgttt accccggtaa
     3601 cgccatcgtg tggctgctga aacaactgct caatacgcgc cacggtggcg tcgagcacat
     3661 cggcgatgtt cttcgggcct tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc
     3721 gggattcagc aaggtccagc agatcttcgc tggtacgccc ctgcggatca aaaccggctt
     3781 cggcaatctc attcgcaacc gagatcatct cacggacaac ggcacgttca cgcacgatgt
     3841 ccgcataggc actgatgttc gccgcacttg gcgtattttt tgacaactct gccagataag
     3901 caaaaccacc gacgctatcg agttgcccct ggcgttccag cgattccgca agggtaatca
     3961 gatcgatagg actaccgctt tcctgcaaac gcgccatttc agtaaagata tgacggtgtg
     4021 ggcgggtgta aaaatcgtct gctaccacac gctcggctac atcatcccag cgttcgttat
     4081 ccagcattaa accgcccaac accgactgct ccgcttcgat cgagtgcgga ggcactttca
     4141 gcccggcaac ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg aagggtttat
     4201 ttcctgccat agtgaatgga gttaccgaga taaagaatgg gtcgaaactt taccatatga
     4261 agcagaccct gacgatacgt tctggaggac acatggcaac acgaattgaa tttcacaagc
     4321 acggtggccc ggaagtactt caagccgtag agttcactcc tgccgatccg gcggagaatg
     4381 aaatccaggt cgaaaataaa gccatcggca tcaattttat cgacacgtat atccgcagcg
     4441 gcctttaccc gccgccatca ctacccagcg gattaggtac cgaagcggca ggcatcgtga
     4501 gtaaagtcgg cagtggtgta aagcatatta aggctggcga tcgtgtagtc tatgcgcagt
     4561 cggcgttagg cgcttacagc tctgtgcata acattaatgc ggataaagcg gcgattctgc
     4621 ctgcggcaat ttcttttgag caagctgcag catctttcct gaaaggctta acggtttatt
     4681 atctgctgcg caaaacctat gaaattaaac ccgatgaaca gttcctgttc cacgcagcag
     4741 ctggcggcgt tggcttgatt gcctgccagt gggcaaaagc cctgggcgcg aaacttatcg
     4801 gcaccgtagg aaccgcgcaa aaagcgcaga gcgcgctaaa agcgggcgcg tggcaggtta
     4861 ttaactatcg tgaagagaat ctggtcgagc ggttaaaaga gatcaccggt ggtaaaaaag
     4921 tgcgcgtagt gtacgattcc gtgggcagag acacctggga acggtcgctg gattgcctgc
     4981 aacgccgcgg cttaatggtg agttttggca actcatcagg tgcggttacc ggtgtgaact
     5041 taggcattct caatcagaaa ggctcgttgt atgtgacacg cccttccctg caaggctata
     5101 tcaccacgcg ggaggaatta accgaggcca gtaatgaact gttctctttg attgccagcg
     5161 gtgtgattaa ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg
     5221 cgcatgagat tctggaaagc cgggcgacgc aaggttccag cctgttgatt ccataaaaga
     5281 aatagggctt ccacctggga agccctttct ttttatagtt cggctgtatg tagggtacag
     5341 cacgatgaat ctgttagagg cgcaatagtg acagatttga ttatcaattc ctattttgtt
     5401 ctaaggataa aaccttaggt tgtgatcatc cgcacaatcc cttagtaatg ccagcggtca
     5461 taacgctgat atttaggcac ttttggtgct ttaatcgcct taataaccca caccaccgca
     5521 atcgccagta gtaaccacgg cagcaactta atcatcaatg ccagcatacc gccgaggaac
     5581 ataatggccg tcgccacaac cagcgcggcg ataatgccca gcaacgaaac gccggtgacc
     5641 atcaacatga caaaaaagcc aatcacaaaa agtagttcca gcatgatgct ctcccaaata
     5701 tgaaatctct tgctggcatt acaagaatca tgccaaaaat aatctattga tttaacagca
     5761 aaacgccccg cgaatttgcg cagggcgtgg tgaatttgac tattttttgg tgaaaagtta
     5821 acgcttatcc gccaccagtt tgagcgcgtg ttccagcaca ttaatgtctg cacccgcttt
     5881 atgggcattt tcacttaaat aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa
     5941 gcccaacata tgccgggtaa tatggccaag atacgtcccc tggctgagtt cacgctcaat
     6001 gtacggatac atggcgcgca ctaccgccac cggatcggca tcggtatccg aggaaccaaa
     6061 gatctcccgg tctaccgccg ccagaatacc cggattctga tacgcctcgc gcccgaccat
     6121 cacgccatcc atatgttgca aatgcgcttt agcttcttcc agcgacttga tgccaccgtt
     6181 aatggacatt gtcagatgcg gaaagtcacg cttcagttga tacacacgcg gataatcgag
     6241 cggcgggatt tcacggtttt ctttcgggct taaccccgaa agccaggctt tacgagcgtg
     6301 gatgatgaac atctcacact cgcctttgcc ggaaacggtg ttgatgaaat cgcagagaaa
     6361 ttcatagctg tcctggtcat cgatgccaat acgcgttttc accgtcaccg gaatcgacac
     6421 cacatcgcgc atcgctttca cgcagtcggc aaccagctgc gcattaccca tcagacacgc
     6481 accaaacatg ccgttctgca cccggtcaga cgggcaaccg acattcaggt tgatctcatc
     6541 atatccacgc gcttctgcca gcttcgcaca ctgcgccagc gccgccggat cgctaccgcc
     6601 gagttgcaac gctaccggat gttcttcttc actgtacgcc aggtaatcac ctttaccgtg
     6661 aataatcgcc cctgtggtca ccatttcggt atacagcagc gtattgcggg aaagcagacg
     6721 caagaaatag cggcaatgtc tgtccgtcca gtcgagcatt ggtgcaacgc taaaccgacc
     6781 actccagtga acgtcagttt tttcaggcat tgcgctggtt tggttgattt tttgcatttc
     6841 agaattaccg tgcattttca aatgtagaga ttattttatc gatatatcat ggagttatgt
     6901 tattcagcat cactgttcag gaggctcaat aacggggtac tataccataa caacaggaag
     6961 cgcctgtctc attgcaaaag aaaaatgaga tcctctcaag gcatgaagct ctcacgaagt
     7021 gaagatctaa tcttattaga tgttagcgtt gtgaaaagca atgataaaca atacagattc
     7081 gacgataaat aaaaatcaca cattaaactc cggtgatatc tctccctgct aatagcactg
     7141 atagagaata aatatcctcc ggcatagccg gaggtttttc aaatgcgcct ataaggctct
     7201 cttaccagcc gcgccctaac aggcgcacac gatctgacat ttgcatccaa cttcgttact
     7261 tacggcccgt aaacgggctg cccggatagg gaatcgataa ctgctctccc attttatcct
     7321 cttcaagctg gtgctttatg taatcctgta tcttcgccgt gttcttaccc accgtatcga
     7381 cgtagtaccc tctgcaccag aactctctgt tcctgtattt gaatttcaaa tcaccaaact
     7441 gctcgtaaag catcagactg cttttccctt tcagatatcc cataaagccg gatacgctca
     7501 ttttgggcgg gatctccaca agcatatgga tatgatctgc acagcattca gcttcaagaa
     7561 tccgtacgct tttccactca cacagctttc tcaaaatact gcctattgct ctacgcttct
     7621 ctctgtagaa cacttgtctt cggtattttg gcgcaaaaac tatgtgatat ttacagttcc
     7681 atcgggtgtg cgctaagctc ttttcgttcc ccattgggac ccccttttga tttcttgttt
     7741 gacacttgca gttgccagac cgcaaggtgt tttaacaaat caaaaggggt tttaataact
     7801 ggctcaaagc tgaaagcttt ccggaacccc cagcctagct gggggttttc tgttcacaaa
     7861 aacaacgggc gtgccatacg cccgtttcaa tacttaacgc acatgtgttt tggtttagtc
     7921 atcatccagt tatatgtatt ttagccagga acaggttaaa cctttcctat ataactcaaa
     7981 aattgaaacc ttattctcat gtcatgctta tattcattat tatcgttata taaaaaggca
     8041 accata
//
LOCUS       DAPGRN010000171         7971 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPGRN010000171 DAPGRN010000000
VERSION     DAPGRN010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.173"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..842
                     /locus_tag="RHW33_004313"
     CDS             117..842
                     /locus_tag="RHW33_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1647755.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSLS"
     gene            800..1477
                     /gene="bglJ"
                     /locus_tag="RHW33_004314"
     CDS             800..1477
                     /gene="bglJ"
                     /locus_tag="RHW33_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV1647756.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1515..2303)
                     /gene="fhuF"
                     /locus_tag="RHW33_004315"
     CDS             complement(1515..2303)
                     /gene="fhuF"
                     /locus_tag="RHW33_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV1647757.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDPTLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNVLSSLLAVYSDHIYRNQPTMIREYKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQHRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2444..2680
                     /locus_tag="RHW33_004316"
     CDS             2444..2680
                     /locus_tag="RHW33_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV1647758.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2719..2805)
                     /locus_tag="RHW33_004317"
     tRNA            complement(2719..2805)
                     /locus_tag="RHW33_004317"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2769..2771),aa:Leu,seq:cag)
     gene            complement(3073..4104)
                     /gene="rsmC"
                     /locus_tag="RHW33_004318"
     CDS             complement(3073..4104)
                     /gene="rsmC"
                     /locus_tag="RHW33_004318"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV1647759.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4207..4620
                     /gene="holD"
                     /locus_tag="RHW33_004319"
     CDS             4207..4620
                     /gene="holD"
                     /locus_tag="RHW33_004319"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV1647760.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4589..5035
                     /gene="rimI"
                     /locus_tag="RHW33_004320"
     CDS             4589..5035
                     /gene="rimI"
                     /locus_tag="RHW33_004320"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV1647761.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5050..5727
                     /gene="yjjG"
                     /locus_tag="RHW33_004321"
     CDS             5050..5727
                     /gene="yjjG"
                     /locus_tag="RHW33_004321"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV1647762.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6113..7348
                     /locus_tag="RHW33_004322"
     CDS             6113..7348
                     /locus_tag="RHW33_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV1647763.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWIVPPERHKTGEITGEPL
                     KRPIIEPVEELIKNAISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIVTCTSLGSNYQFE"
     gene            complement(7511..>7971)
                     /locus_tag="RHW33_004323"
     CDS             complement(7511..>7971)
                     /locus_tag="RHW33_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV1647764.1"
                     /translation="EDRAFLWDRIGHWAQDEDEWEDAEMYFRKAYEMAGGEYGYCLGV
                     ALNHLGRFKESLPILIEQAEKIQPDDFSWFQVAIAYEFQGYIQEAINSYKKCLAINEK
                     NELALFNLGGIYWNSRNYDEARHIWKLAVEYYPDHELSRKLRKDIPFILD"
BASE COUNT         2083 a         1871 c         1978 g         2039 t
ORIGIN      
        1 aaagtgaatg ctaaggataa tttattcgct taatctatta atttgctgga aacatttaag
       61 gaatgaatat cctgaataaa ccatcataat ttttagacag tggatgtgga ggaaatatgt
      121 tgccaggatg ctgcaaaaat ggaattgtta tcagtaaaat acctgttatg caagcagggt
      181 taaaagaggt catgaggact cacttccctg aatatgaaat aatatccagc gcctctgcgg
      241 aggaccttac cttattacaa ttacgtcgtt ccggattagt cattgctgat ttagccggtg
      301 aaagtgaaga tccacgttct gtttgtgaac attattattc tttaatctca caatatcggg
      361 aaattcactg ggttttcatg gtgtcacgct cctggtattc ccaggcagta gaactgctca
      421 tgtgccctac ggcgacgtta ttgtctgatg ttgaacccat tgagaatctg gtcaagaccg
      481 tacgttccgg caatacgcac gcagagcgta tcagcgccat gctgacctcc ccggcaatga
      541 ctgaaactca tgattttagc tatcgctccg tcattctcac tctttcagag cgcaaggtac
      601 tgcggctatt aggtaaagga tggggcatca accagatagc ttcattgctt aagaaaagta
      661 ataaaactat cagcgcccaa aaaaacagtg cgatgcgtcg actggcaatt cacagcaacg
      721 ctgaaatgta tgcatggata aatagcgcgc agggtgcaag agaacttaac ttgccttctg
      781 tttatggaga tgccgcagaa tggaacacag ccgaattaag aagagaaatg tcgctctcat
      841 agaaaaatgc gtcatgagta gtatcggtat tgagagttta ttcagaaagt ttgcgggtaa
      901 cccttataag ctccatacct ataccagtca ggagtcattt caggatgcca tgtcgaggat
      961 ctcgtttgcg gcggtcattt tttctttttc tgccatgaga agtgagcgca gagagggatt
     1021 atcttgcctg actgaactgg cgattaagtt tccgcgtacc cggcgtttag ttattgcgga
     1081 tgatgatatt gaagctcggc tgattggttc attgtcgcca tcaccgctgg acggtgtatt
     1141 aagtaaagcg tcaacgctgg agatttttca tcaggaactc tttttgtcat taaatggtgt
     1201 acgtcaggcg accgaccgac tgaacaatca gtggtacatt aaccaaagcc ggacgttaag
     1261 cccgacggag agagaaatat tgcgctttat gtcgcgtggc tactcaatga cacaaattgc
     1321 cgagcagctt aaacgcaata tcaaaacgat ccgtgcacat aaatttaatg tgatgtcgaa
     1381 actgggcgtc agttctgacg cagggttgtt ggaggccgca gatattctgt tatgtatgcg
     1441 gcattgcgaa acaagtaatg tgttgcatcc ctattaatcc gcatgatgcc gggtttactt
     1501 ccccggcagt gctttcattt cagcgtacaa tcgccacatt gctgcacatc cggtaagcga
     1561 taacgctggc agcaagtgcg gcgcaccagc aggccgtcgc gtagcaccac ggtacgccag
     1621 agtggattat cttcaccgtt cgtgagcgtt ttctcaaaaa agagggcatg acgcagcgat
     1681 tcaaccgtcg cctcgccgag cagttgcttc atctcagtga gataccagtt gatcaaataa
     1741 ccggtattac tccagataag tttgccgtta atctctcccg ttgcttctag tgcctgcaca
     1801 accggaacca gcgcctggct gattaacgtt tccattcgat gctgcggcga atgtggtgtt
     1861 gcgtttttat cttcacatac atcgacccag aaacaggcgg cgcgtccggt ttcgtgaaac
     1921 tcaacgtgga aatgttccgg cgacacatct aatgcttttt cctgcgtcag cagcgccagc
     1981 attaatggcg gcaccatcag gccgatatac cattgtgccc atagtgagat cagcggtttg
     2041 tactcgcgga tcatcgttgg ttggttgcga tagatatgat cggaatagac cgccagcaga
     2101 gaacttagca cattcggtga tgaccattgc gccagcgtca tggcgttaag tggggcaggt
     2161 tcatccaggc ggataaactc cagcaaatgt tcacgatgtt ttgcgatcgt cgcccgcacg
     2221 gcttgcgcaa gcgtgggatc ctgcggctgg agatgcgttc gccagataac atcttcatag
     2281 agcggtgcgg aacgataggc cataatcggg atagtaatct aaatgataat gattgctaat
     2341 cctagcgata ggtttacccg atagcaaggg atttctctgg cttgcaaatg ataaaaatta
     2401 tcatatgata ttggttatca ttatcaatga aagagataaa accatgttgc aacgtatgct
     2461 gggcagtggc tggggagtgt tgctgccggg attgctgatt gcagggctga tgtatgcgga
     2521 tttatcgccg gatcagtggc ggattgtcat tctgatggga ttagtattga cgccggtgat
     2581 gctgtatcac aaacagttgc ggcattacat tttgctacca tcgtgcctgg cgcttatcgc
     2641 tggcatcatg ctgatgataa tgaatttgaa tcagggatga caaaacaggg aagaaacaag
     2701 aaaggaagta aagataattg gtgcgagggg ggggacttga acccccacgt ccgtaaggac
     2761 actaacacct gaagctagcg cgtctaccaa ttccgccacc ttcgcatacc atcaattctt
     2821 aaaaagaatt gctaccacgg aggcgcattc tagtggtttt cagcttttcg tcaatagtta
     2881 attatcgcct gatgtgtaat tgctggaaaa atgtccatca ggaaactagc gtgcaggttt
     2941 ggtaggcatg cgggggcaga tgccagatgc gacgctggcg cgtcttatct ggcctacgaa
     3001 gggctaacgt gcaggttttg taggtcggat aaggcgttca cgccgcatcc gacacggtat
     3061 tcggcgagat aattaacctt tcttcgcctg gcgggtcata atggcgcgat acaccttgaa
     3121 acgcccggtt tgcgcaatca cttcatggaa gccaaatgtc tcatccagca cgtccggata
     3181 aggcaggaag gcgttcgcta caattcgcag ctcgccgccg ctattaagat gacgcaccgc
     3241 gccgcgaatc agcgtttgcg ccgcatccag gctggtttgc atcccatcgt ggaacggcgg
     3301 gttggagatg atcatatcaa aacgaccttt cacctcggaa aagacgttgc tggcaaagac
     3361 ttcaccttca acgccgttgg cggcaagtgt tgcgcggctg gcttctaccg ccggggcaga
     3421 gacatcgcac agagtgagac gaattttcgg cgaatggcgc gcaaaggcaa ctgaaagcac
     3481 acccgcgcca cagccgacat ccagcacttt acctttcgtg tgcggggtta acgtcgagag
     3541 cagcaactgg ctaccgacat ccagaccgtc gcggctaaac acgccaggca gcgttttgac
     3601 cgtcaggcca tcgacgctgt attcgcccca gaatttatcg gcatcaaata ccggctgttt
     3661 ttccagacga ccaaaataga ggccacagcg acgagcgctg tcgactttat tcaacggcgc
     3721 ataatctgcc agcatctgct cggcgctgcg cacgccgctg cggttctcgc caacgacaaa
     3781 aatatctgtc cccactggca gcagagaaag taaattcatc aactggaact gggcttccgg
     3841 tttgttcttc ggccagtagt aaatcagcgt atcgcaatct gcgacgtcat ccgccgtggc
     3901 gaccagactg aaacgggcgt tatcccccat ctggcggctt aatacctgcc agtggtggaa
     3961 ttgctgggta tgagcacggc tggccgcggt atctaaacgc gcgggcaggt catcctgtaa
     4021 gtctccggca aacagaatac ggctttgttc gaaatcatca ctgtgacgca gcaagacttc
     4081 acttgccggg gtaaatgcag acatggaatg ctcctcaatt gatactggcg gcgattatag
     4141 ccatatgttg gcgcggtatc gacgaatttg ctatatttgc gcccctgaca acaggagcga
     4201 ttcgctatga catcccgacg agactggcag ttacagcaac tgggcattac ccagtggtcg
     4261 ctgcgtcgcc ctggcgcgtt gcagggggag attgccattg cgatcccggc acacgtccgt
     4321 ctggtgatgg tggcaaacga tcttcccgcc ctgactgatc ctttagtgag cgatgttctg
     4381 cgtgcattaa ccgtcagccc cgaccaggtg ctgcaactga cgccagaaaa aatcgcgatg
     4441 ctgccgcaag gcagtcgctg caacagctgg cggttgggta ctgacgaacc gctatcactg
     4501 gaaggcgctc aggtggcatc accggcgctc accgaattac gggcaaaccc aacggcacgc
     4561 gccgcgttat ggcaacaaat ttgcacatat gaacacgatt tcttccctcg aaacgactga
     4621 tttaccagcg gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga gtgaaaaaac
     4681 ctttgccagc aaccagggcg agcgttatct caactttcag ttaacgcaaa acggcaaaat
     4741 ggcggcgttt gcgattacgc aagtggtgct ggatgaagct acattgttca atattgcggt
     4801 cgatcctgac tatcagcgtc agggattggg aagggcgctg ctggaacatc tgatcgacga
     4861 actggaaaaa cgcggcgtgg cgacgctctg gctggaagtc cgtgcctcaa acgctgccgc
     4921 cattgccctg tacgaaagtt taggctttaa cgaggcgacg attcgccgca attactaccc
     4981 caccacggac ggtcgcgaag acgccatcat catggcgttg ccaatcagta tgtaagacaa
     5041 ggtggaataa tgaagtggga ctggattttc tttgatgccg atgaaacgct gtttaccttt
     5101 gactcattca ccggcctgca gcggatgttt cttgattaca gcgtcacctt taccgctgaa
     5161 gattttcagg actatcaggc cgttaacaag ccactgtggg tggattatca aaacggcgcg
     5221 atcacttcat tacagcttca gcacgggcgg tttgagagct gggccgaacg gctgaacgtc
     5281 gagccgggta aactcaacga agcctttatt aatgcgatgg cggaaatctg tacgccgctg
     5341 ccgggcgcgg tttctctgct taacgccatt cgtggcaacg ccaaaatcgg catcatcacc
     5401 aacggtttta gcgccttgca gcaagtgcgt ctggaacgga cgggcctgcg tgattacttc
     5461 gatttgctgg tgatttccga agaagttggc gttgctaaac cgaataagaa aattttcgat
     5521 tatgcgctgg aacaggcggg caatcctgac cgttcacgcg tgctgatggt tggcgacact
     5581 gccgagtccg atattctcgg tggcatcaac gccgggcttg cgacctgctg gctgaatgca
     5641 caccatcgcg agcaaccaga aggcatcgcg cccacctgga ccgtttcttc gttgcacgaa
     5701 ctggagcagc tcctgtgtaa acactgattg cctccccccc gttgatgggt aaaatagccg
     5761 caatttttcg ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga aagaattatg
     5821 acgttgtctc cttatttgca agaggtggcg aagcgccgca cttttgccat tatttctcac
     5881 cccaataatt aagcccaaat taaagctctt ttactctttc aaaatccttt cagttaattg
     5941 aggttgagct atataactca tttaaataca tttagttatg tggcttcttt aattgaaaat
     6001 tctttcatgt aactttaaat cgatctgctt gctttcatca aaaatctgta catatgcttg
     6061 tacataatgt acaaagcagc agaggtgttt tgcgatttgt acaaggtgag taatggcgct
     6121 gtctgatgcg tggttgcgtt cagtcgttgg aaaggaacgt gataaggttt tggttaaatc
     6181 cgatcgtgat ggtttgtctg tcagagtatc accgaaaggt cgcgtagtgt tccaatatcg
     6241 ttatcaatgg gcagggaaag gtgagcgtct tgatatcgga acttacccgg caactggatt
     6301 aaaagaggcc agagaagaag ttatccgtct ccgtggtgaa ctcgagtcaa accgtaatcc
     6361 acgattggtc aagcaggctg aaaaacgaaa agctactgaa gccatgacgg tagagtctgt
     6421 gatccgtgcc tggtatgaag catattgtgt aaaaaataaa aaaggctctg aacagatact
     6481 ccgctcgttt gagctgcacc tgttctctaa aatcgggaat atccctcacg atgcagctac
     6541 attgcatgat tggttagaag tcctggagcc tcttagcact aagactccag caatagcaga
     6601 ccgattgcta attaacgcaa agcaggccca tgtctgggcg tataagagaa agctcattga
     6661 aactcgcccg ctgtcggata tcacgggtaa agatatggat atccgtaaag gtcagaagaa
     6721 acggtttctg acacatgatg aaattaaaat cctttatgct gcgatcgatg gttctcgaat
     6781 ggttcctaaa taccgggcct tcattaagct attgctgcat tttggctgtc gtagttcaga
     6841 gctaattact gctagggtgg acgattttga tttcattaat aaggtatgga ttgtaccacc
     6901 tgaacgacat aagactgggg agataacggg cgaaccgcta aagcggccca ttattgaacc
     6961 ggtggaagag cttataaaga acgctatctc tatgaacaat ggttccgata tgctttttac
     7021 taaggaagga agcagggaac ccgttggtcg aacatcattg cagtcgctgc cttacaattt
     7081 aatgcagtac gcatggcggc gtttggggta tcaattccct cattggtctc ttcatgattt
     7141 gaggcgaaca gcacgaacaa atttttctga tcttactgct cctcatattg cagaaataat
     7201 gctcggccat aaactgccag gggtttggca ggtttatgac aagagcgatt atctagaaga
     7261 acagcgtaaa gcctaccagg cgtggtggga gagggttgaa tcgatcgtta cttgtactag
     7321 cttaggctct aactatcagt tcgagtagct aatacgcaat caaatatgat agcccatttt
     7381 gagaagagga atagtcattt aagtgggatg cagctatcca ctacaatatg actctatagc
     7441 aacgagctta ttccatatat tcatgacaag ttgaataaat tatgactttt agctcgttgc
     7501 tcaaaagaga tcaatctaaa ataaatggaa tatcctttcg aagcttacgc gaaagctcat
     7561 ggtcaggata atattctact gctagtttcc agatatgtcg tgcttcatca taatttctcg
     7621 aattccagta aatgcctcca agattgaaca atgctagttc gtttttctca ttgattgcta
     7681 aacatttctt gtaagagtta atagcctcct gaatataccc ttgaaattcg taggcaatag
     7741 cgacttgaaa ccagctaaaa tcatcaggtt ggattttttc cgcttgttca atcaaaatgg
     7801 gcaagctttc tttaaagcga ccaagatgat tcagagccac accgagacaa tatccatatt
     7861 caccccctgc catttcataa gctttacgaa aatacatctc agcatcttcc cactcgtctt
     7921 catcctgtgc ccaatgacct atacgatccc ataagaaagc cctatcttca c
//
LOCUS       DAPGRN010000172         7926 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPGRN010000172 DAPGRN010000000
VERSION     DAPGRN010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.174"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            188..1300
                     /locus_tag="RHW33_004324"
     CDS             188..1300
                     /locus_tag="RHW33_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1647765.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNDKVDGDKTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALEQNGDGVGGSITYDIGEGFSFGGAISSS
                     KRTAEQNNSARFIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATLAGDLGWANKAQ
                     NFEVVAQYQFDFGLRPSVAYLQSKGKNLNANIAGRTYDDEDLVKYVDVGATYYFNKNM
                     STYVDYKINLLDDNNFTRAAGINTDDIVALGLVYQF"
     gene            1412..2467
                     /gene="apbE"
                     /locus_tag="RHW33_004325"
     CDS             1412..2467
                     /gene="apbE"
                     /locus_tag="RHW33_004325"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV1647766.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPDIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPISEA
                     MAEIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2541..3605
                     /gene="ada"
                     /locus_tag="RHW33_004326"
     CDS             2541..3605
                     /gene="ada"
                     /locus_tag="RHW33_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV1647767.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3605..4255
                     /gene="alkB"
                     /locus_tag="RHW33_004327"
     CDS             3605..4255
                     /gene="alkB"
                     /locus_tag="RHW33_004327"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV1647768.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4331..5974
                     /gene="yojI"
                     /locus_tag="RHW33_004328"
     CDS             4331..5974
                     /gene="yojI"
                     /locus_tag="RHW33_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV1647769.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6192..7838
                     /gene="mqo"
                     /locus_tag="RHW33_004329"
     CDS             6192..7838
                     /gene="mqo"
                     /locus_tag="RHW33_004329"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV1647770.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT         1902 a         1969 c         2175 g         1880 t
ORIGIN      
        1 cccttctatt tacattttga aacatctata gcgataaatg aaacatctta aaagttttag
       61 tatcatattc gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta
      121 atgagggtta atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt
      181 taataacatg aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc
      241 agcaaacgct gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt
      301 agacggcctg cactatttct ccgacaacga taaggttgat ggtgacaaga cctacatgcg
      361 tcttggcttc aaaggtgaaa ctcaggttac tgaccagctg accggttacg gccagtggga
      421 atatcagatc cagggcaacg ctcctgaaag cgaaaacaac tcctggaccc gtgtggcatt
      481 tgctggtctg aaattccagg acgttggttc tttcgactac ggtcgtaact acggcgttgt
      541 ttacgacgta acctcctgga ccgacgtgct gccagaattc ggtggcgaca cctacggttc
      601 tgacaacttc atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ccgacttctt
      661 cggtctggtt gacggcctga actttgctgt tcagtaccag ggcaaaaacg gtagcgtaag
      721 cggcgaaggc atgaccaaca acggtcgtga tgctcttgag cagaacggtg acggtgttgg
      781 tggttctatc acttatgaca tcggtgaagg cttcagcttc ggtggtgcga tctccagctc
      841 caaacgcacc gcagagcaga acaacagtgc tcgtttcatc ggtaatggtg atcgtgcaga
      901 aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg ctcagtacac
      961 ccagacttat aacgcaactc tggctggtga tttaggctgg gcgaacaaag cccagaactt
     1021 tgaagtggtt gcccagtatc agttcgactt cggtctgcgt ccttctgttg cttacctgca
     1081 gtccaaaggt aaaaatctga atgccaacat cgctggtcgt acttacgacg acgaagatct
     1141 ggtgaaatat gttgatgttg gtgctaccta ctacttcaac aaaaacatgt ccacctatgt
     1201 tgattataaa atcaacctgc tggacgacaa taacttcacc cgcgctgctg gcatcaacac
     1261 tgatgacatc gtagctctgg gtctggttta ccagttctaa tctcgatttc tatcgaacaa
     1321 agggcctgcg ggcccttttt tcattgtttt cagcgtacaa actcagtttt ttggtgtact
     1381 cttgcgaccg ttcgcatgag gataatcacg tatggaaata agctttaccc gcgtggcact
     1441 gctggctgcc gcgctcttct ttgttggttg cgatcaaaaa ccacaacccg ccaaaaccca
     1501 cgctactgaa gttaccgttc ttgaaggcaa aactatgggt accttctggc gtgccagcat
     1561 cccggacatt gacgccaaac gcagcgccga acttaaagaa aagattcaga cccagctgga
     1621 cgccgacgat cagctgcttt cgacctataa aaaagattcc gcgctgatgc gctttaacga
     1681 ttcgcaaagt ttatcgccgt ggccgataag tgaagcgatg gccgaaatcg tcactacctc
     1741 gctgcgcatt ggcgcaagga ccgatggcgc gatggatata accgtcgggc cgctggtgaa
     1801 tctgtggggc tttggcccgg aacaacagcc ggttcaaatt ccgagccagg aacagatcga
     1861 tgcgatgaaa gccaaaaccg gcttacagca cctgacggtc attaatcagt cgcatcagca
     1921 atatctgcaa aaagacctgc cggatttata tgtcgatctc tccaccgtcg gtgaaggtta
     1981 tgcggcggat cacctggcac gcttgatgga gcaggaaggg atttcccgct atctggtgtc
     2041 ggtgggcggc gcgctgaaca gccgtggtat gaacggtgaa ggccagccgt ggcgggtagc
     2101 gattcaaaag ccaaccgata aagaaaatgc ggttcaggcc gtggtggata tcaacggtca
     2161 tggtattagc acctctggca gctaccgtaa ctattacgaa ctggacggca aacgtctttc
     2221 ccatgttatc gatccgcaaa ccgggcgtcc catcgaacac aatctggtat ctgtgacggt
     2281 gattgctccg acggcgctgg aagccgatgc ctgggatact ggcttgatgg tcctcgggcc
     2341 ggagaaagcc aaagaggttg ttcgccggga agggctggcg gtttatatga tcaccaaaga
     2401 aggcgatagc tttaaaacct ggatgtcacc acagtttaaa agcttccttg tcagcgaaaa
     2461 aaattaaagc gcaagattgt tggtttttgc gtgatggtgg ccgggcagac taaaggctat
     2521 ccttaaccag ggagctgatt atgaaaaacg ccacatgctt aactgacgat caacgctggc
     2581 aatctgtctt agcccgcgac ccgaatgccg acggcgaatt cgttttcgcc gtgcgcacca
     2641 caggcatctt ttgccgtccg tcttgccgcg ccagacatgc tttgcgggaa aacgtctcct
     2701 tctacgcaaa tgccagcgag gcactcgccg ccggatttcg cccctgcaaa cgctgccaac
     2761 ctgataaagc caatgcccag caacatcggt tggataaaat cacccacgcg tgtcgactgc
     2821 tggaacagga aacgcctgta acgctggaag ccttagccga ccaggtggcg atgagtccgt
     2881 tccatctgca tcggttgttt aaagcgacta ccggaatgac gcctaaagcc tggcaacagg
     2941 cctggcgcgc tcgccgtttg cgcgaatcgc tggcgaaagg ggagagcgtg acgacgtcta
     3001 ttcttaatgc cggattcccc gacagcagca gttactaccg caaagccgat gaaacgctgg
     3061 gcatgacggc taaacaattc cgtcacggtg gcgaaaatct ggcggtgcgt tacgcgctgg
     3121 ctgattgtga gctgggtcgt tgcctggtgg cagaaagcga gcgggggatt tgcgcgatat
     3181 tgctgggcga tgatgacgcg acactaatca gcgagttgca gcagatgttt cccgctgccg
     3241 acaacgcgcc tgccgatctg acgtttcagc aacatgtgcg tgaagtgatc gccagcctca
     3301 atcaacgcga tacgccgctg acgttaccgc tggatattcg cggcactgct tttcagcaac
     3361 aagtctggca ggcactgcgc acgatacctt gcggtgaaac cgtcagttat cagcaactgg
     3421 ctaacgccat cggcaaaccg aaagcggtac gggccgttgc cagcgcctgt gccgccaaca
     3481 agctggctat cgtaataccc tgtcatcgag tggtccgtgg tgatggcaca ctttccggtt
     3541 accgctgggg cgtgtcgcgt aaagcgcaac tgctgcgccg cgaagctgaa aatgaggaga
     3601 ggtaatgttg gatctgtttg ccgatgctga accgtggcaa gagccactgg cggctggtgc
     3661 ggtaatttta cggcgttttg cttttaacgc tgcggagcaa ctaatccgcg atattaatga
     3721 cgttgccagc cagtcgccgt ttcgccagat ggtcaccccc gggggatata ccatgtcggt
     3781 ggcgatgacc aactgtgggc atctgggctg gacgacccat cggcaaggtt atctctattc
     3841 gcccattgat ccgcaaacaa ataaaccgtg gcccgccatg ccgcagagtt ttcataattt
     3901 atgtcaacgt gcggctacgg cggcgggcta tccagatttc cagccagatg cttgtcttat
     3961 caaccgctac gctcctggcg cgaaactgtc gctgcatcag gataaagacg aaccggatct
     4021 gcgcgcgcca attgtttctg tttctctggg cttacccgcg atttttcaat ttggtggcct
     4081 gaaacgtaat gatccgctca aacgtttgtt gttggaacat ggcgatgtgg tggtatgggg
     4141 cggtgaatcg cggctgtttt atcacggtat tcaaccgttg aaagcggggt ttcatccact
     4201 caccaccgac tgccgctaca acctgacatt ccgtcaggca ggtaaaaaag aataaaaata
     4261 agaattatta ttgctgtgcg cgcgaagatg tttaaactgc gggctgtaat tcattgtccg
     4321 ggttttctgc atggaacttc ttgtacttgt ctggcggcag tatcgctggc catttatcag
     4381 tgtgatggcg ctaagcctcg ccagtgcggc tttaggcatt ggcttaattg cttttatcaa
     4441 tcagcgcctt atcgaaacgg cggataccag tctgctggtg ttgccggagt ttctgggatt
     4501 attgctgctg ttgatggcag tcactctcgg atcgcaactg gcgctcacca ctttggggca
     4561 tcacttcgtt taccgactgc gtagtgaatt tatcaagcgg attctggata cccacgtcga
     4621 gcgcattgaa caactcggta gcgcctcgtt gctggcggga ttaaccagcg atgtgcgcaa
     4681 tatcaccatt gcttttgtgc gtctgccgga actggtgcag gggatcattc tcactatcgg
     4741 ttcagcggcg tatctgtgga tgctgtcggg caaaatgttg ctggtaacgg ctatctggat
     4801 ggcagtcacc atctggggcg gttttgtgct ggtggcgcgg gtgtacaaac atatggcgac
     4861 cctgcgtgaa accgaagaca agctgtacac ggattttcaa actgtacttg aagggcgcaa
     4921 agagctgact ctgaaccggg aacgcgccga gtatgtgttt aacaacctct acattcctga
     4981 tgcgcaagag tatcgccatc atattattcg cgcagacacc ttccatctta gtgccgtgaa
     5041 ctggtcaaac atcatgatgc tgggcgctat cggcctggtg ttctggatgg cgaacagtct
     5101 cggttgggct gataccaacg ttgccgcgac ctattcgttg acgcttttat tcctgcgtac
     5161 gccgctgctt tcggcggttg gcgcattgcc gacgctgctg acggcgcagg tggcgtttaa
     5221 caagctgaac aaattcgcgc tcgcgccttt caaagcagaa tttccgcgcc cgcaggcgtt
     5281 tccaaactgg caaacgctgg agctgcgtaa tgtgacgttt gcttatcagg ataacgcgtt
     5341 ttccgttggt ccgattaatc tcaccatcaa acgtggcgag ctgctgtttc tgattggcgg
     5401 caacggtagc ggaaaatcga cgctggcgat gttgttgacg ggcttgtatc agccacaaag
     5461 cggcgaaatc ttgctggatg gcaaacctgt cagcggcgaa caaccggaag attatcgcaa
     5521 actgttttcg gcagtgttta ccgatgtctg gctgtttgat caactgctgg ggccggaggg
     5581 taaacccgct aacccgcaac tggttgagaa gtggctggcg cagctgaaaa tggctcataa
     5641 gcttgagtta agcaacgggc gtattgttaa cctgaagtta tcaaaagggc agaaaaaacg
     5701 cgtggcgctg ttgctggcgc tggcagaaga acgcgatatt atcctgctgg atgaatgggc
     5761 ggcggatcag gatccacact tccgtcgtga gttttatcag gtgttgctgc cgctgatgca
     5821 ggagatgggt aaaactattt tcgccatcag tcatgatgat cattacttta tccacgccga
     5881 ccgcctgctg gaaatgcgca acgggcaact tagcgagctg acgggcgaag agcgcgatgc
     5941 cgcttcgcgt gatgccgttg cccggacggc ataatattac gacgctgacc tggcggactg
     6001 ctgccgccag gtcaatatgc taaaaatccc ttcatttcta ataaccctat aattaatcaa
     6061 cgaaattata atgtttctaa aattagaata taatttataa acattattta aatgttgtta
     6121 cttaagtgtt accgttgatg ccgcgcaaat ctcacctgca ataaagcgac taaaagtaag
     6181 gcattaacaa gatgaaaaaa gtgactgcca tgctcttctc gatggccgtg gggcttaatg
     6241 ccgtttcgat ggcggcaaaa gcgaaagcgt ccgaggagca ggaaactgat gtactgttga
     6301 ttggcggcgg cattatgagc gccacgttgg ggacctattt acgcgagctg gagcctgaat
     6361 ggtcgatgac catggtggag cgcctggagg gggtcgcgca ggagagttcg aacggctgga
     6421 ataacgccgg aaccgggcat tctgcactga tggaactgaa ctacaccccg caaaacgccg
     6481 atggcagcat cagtattgaa aaagcagtcg ccattaacga agcatttcag atttcccgcc
     6541 agttctgggc gcaccaggtc gagcgcggcg tgctgcgtac tccgcgttca tttatcaata
     6601 ccgttccgca tatgagcttt gtctggggcg aggataacgt taatttcctg cgcgcccgtt
     6661 acgccgcgtt gcaacaaagc tcgctgtttc gcggtatgcg ttactctgaa gatcacgcgc
     6721 agatcaaaga gtgggcaccg ttagtgatgg aagggcgcga tccgcaacag aaagtggcag
     6781 ccacgcgtac ggaaattggt accgatgtga actacggcga gatcacccgc cagttaattg
     6841 cttccttgca gaagaaatct aacttctcgc tgcaactcag cagcgaagtc cgcgccctaa
     6901 agcgtaatga cgataacacc tggaccgtta ccgttgccga tctgaaaaat ggcactgcac
     6961 agaacattcg tgccaaattt gtctttatcg gcgcgggcgg tgcggcgctg aagctgttac
     7021 aggaatcggg gattccggaa gcgaaagact acgccggttt cccggtgggc ggacagttcc
     7081 ttgtttcgga aaacccggac gtggttaatc accatctggc gaaggtttac ggtaaagcat
     7141 ccgttggcgc accaccgatg tcggttccgc atatcgatac ccgtgttctg gacggtaaac
     7201 gcgtagtgct gtttgggcca tttgccacct tctcaaccaa attcctcaaa aacggttcat
     7261 tgtgggatct gatgagttcc accaccacct ctaacgtgat gccgatgatg cacgtcgggc
     7321 tggataattt cgatctggtg aaatatctgg tgagtcaggt gatgttgagt gaagaggatc
     7381 gttttgaagc gttgaaagag tactatccgc aagcgaaaaa agaggactgg cgtttgtggc
     7441 aagcggggca gcgtgtgcag attatcaagc gtgatgccga taaaggtggt gtactgcgtc
     7501 tgggtactga agtggtcagt gaccagcaag gaaccattgc cgcgctcctg ggggcatcgc
     7561 caggggcgtc aaccgccgcg ccgattatgt tggatctgct ggaaaaagta tttggcgatc
     7621 gcgtttccag cccgcaatgg caggctacgt tgaaagctat cgttccgtct tatggacgca
     7681 agctgaacgg tgatgtagcc gcaacagagc gcgagttgca gtacaccagc gaagtgctgg
     7741 ggctgaaata cgacaaaccg caagcagcag atagtacgcc gaaaccgcag ttgaaaccgc
     7801 aacccgttca aaaagaagtg gcggatattg cgttgtaatg atgtgccaca tccggcatgg
     7861 tatgccggat gtggcgtatg ctgataagac gcgccagcgt cgcatcaggc aaccggctcg
     7921 ggcgtt
//
LOCUS       DAPGRN010000173         7874 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPGRN010000173 DAPGRN010000000
VERSION     DAPGRN010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.175"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..3863
                     /gene="espP"
                     /locus_tag="RHW33_004330"
     CDS             <1..3863
                     /gene="espP"
                     /locus_tag="RHW33_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HDV1647771.1"
                     /translation="GGLIAVSELSGRVSSRTTGKKKHKRILALCFLGLLPSSYSFASQ
                     MDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPFPDFSPVANKGSTT
                     SIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVSRLDKFVVETRGAT
                     EGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRITPFNEVSYKPEMLN
                     GSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKKWVVAGTVWGIYNY
                     ANGKNHAAYSKWNQTTIDNLKNKFSYKVDMSGAQVATIENGKLTGTGADTTDIKNKDL
                     IFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDTRNGSIVEWNIRYD
                     NKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITSGDGTVRLNAENAL
                     SGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKKSVLSLNNTADYIY
                     HGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQGHATEHAIYRDGA
                     FSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDLSQPDWETGTFRFG
                     TLHLENSDFSIGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNITGDGFGFRQNIVR
                     GNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEKGADVTTQSGMFST
                     SDISVSGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKNKASVVGDIHSTKS
                     ASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSASATMNNTWWQLTGD
                     SALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTDLKNSDKLVVNKKLS
                     GKDNILLVDFLNKPSGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITTRETDD
                     KITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGDLRDINGEAGAWAR
                     IMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKS
                     VGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYH
                     VTEDTWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKD
                     WKVTARAGLGYQFDLLVNGETVLRDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGL
                     EFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4034..4311)
                     /locus_tag="RHW33_004331"
                     /pseudo
     CDS             complement(4034..4311)
                     /locus_tag="RHW33_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4398..5048
                     /locus_tag="RHW33_004332"
     CDS             4398..5048
                     /locus_tag="RHW33_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV1647772.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
     gene            5048..5395
                     /gene="tnpB"
                     /locus_tag="RHW33_004333"
     CDS             5048..5395
                     /gene="tnpB"
                     /locus_tag="RHW33_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV1647773.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWSVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            5415..6986
                     /locus_tag="RHW33_004334"
     CDS             5415..6986
                     /locus_tag="RHW33_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV1647774.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQELISALQEEMAETLGEQYDPVLPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACSGELSPLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGVHPQNHLAGYSGVLQADAYGGYRVLYESG
                     RITEAACMAHARRKIHDVHARMPTDITTEALQRIGELYAIEAEVRGCTAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAVVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLTSQ"
     gene            complement(7018..7874)
                     /locus_tag="RHW33_004335"
                     /pseudo
     CDS             complement(7018..7874)
                     /locus_tag="RHW33_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
BASE COUNT         2086 a         1952 c         1976 g         1860 t
ORIGIN      
        1 caggagggtt aatcgctgtt tctgaattat ccggcagagt atcatcaaga acaactggta
       61 agaaaaaaca caaacgcata cttgcattat gttttttagg cttattacca tcctcatatt
      121 cttttgcgtc acagatggat atttcaaatt tctacatccg tgactatatg gattttgcac
      181 agaacaaggg catatttcag gctggcgcaa caaatattga aatagtgaag aaagatggct
      241 ccaccctgaa actaccggaa gtaccatttc ctgacttctc accggttgca aacaaagggt
      301 caaccacatc tattggtggt gcatacagta tcacagccac acacaatacg aaaaaccacc
      361 actcagttgc gacgcaaaac tgggggaaca gcacgtacaa acaaactgac tggaatactt
      421 cacatcctga ttttgcagta tcccgacttg acaagtttgt tgttgagacc cgaggtgcga
      481 ctgaaggcgc agatatttcg ttatcaaaac agcaggcact tgaacgttac ggggttaatt
      541 ataaaggaga aaagaaactt atcgcattca gagccggctc tggtgtggta tccgttaaaa
      601 aaaatggacg cataactcca tttaatgagg tttcttataa gccagaaatg ttaaatggct
      661 ctttcgttca cattgatgac tggagtggat ggctgatatt aaccaacaac cagtttgatg
      721 agtttaataa cattgcctct cagggtgaca gcggttcagc actgttcgtc tatgataacc
      781 aaaagaaaaa gtgggttgtc gctggaactg tatgggggat ttataattac gccaatggca
      841 aaaaccacgc agcatacagt aaatggaacc agacaaccat tgacaatctg aagaacaagt
      901 tttcttacaa agtggatatg tcaggagctc aggttgcaac cattgaaaat ggaaaactga
      961 caggcactgg cgcagacacc accgatataa aaaataagga cttaatattt actggcggtg
     1021 gagatatcct cctgaaatcc tcttttgata atggtgctgg cggtcttgtc tttaatgata
     1081 aaaagaccta tcgagtaaac ggggatgatt tcacctttaa aggtgccggt gttgatacaa
     1141 gaaacggcag catcgttgag tggaatatcc ggtatgataa taaagacaac cttcacaaaa
     1201 ttggtgatgg cacattagat gtccgaaaaa cccagaacac caacctgaaa acaggtgagg
     1261 gtcttgtcat tcttggcgct gaaaaaacat tcaataatat ctacataacc agtggtgatg
     1321 gaactgtccg actgaatgca gaaaatgcac tgtctggcgg tgaatacaac ggtattttct
     1381 ttgcgaaaaa tggcggaact cttgacctga acggatataa tcagtctttc aataaaattg
     1441 ctgcaactga ttcaggtgct gtaataacca atacgtcaac caaaaagtcc gttttatccc
     1501 tgaataatac tgctgactat atctatcacg gcaacataaa cgggaatctg gacgtacttc
     1561 agcatcatga gacgaaaaaa gagaaccgtc gtcttattct tgatgggggg gtggacacaa
     1621 caaatgatat aagcctgcgc aatacacaac tgtccatgca gggacatgcc actgaacatg
     1681 ccatttatcg ggatggtgct ttctcttgtt cactaccagc gcctatgcgc tttttgtgtg
     1741 gcagtgatta tgttgcagga atgcaaaata cagaagctga tgctgtaaaa caaaacggaa
     1801 atgcctataa aaccaacaat gctgtctctg atttatcgca gccagactgg gaaaccggaa
     1861 cattcagatt tggaacgcta catcttgaaa attccgattt ttctattggt cgtaatgcaa
     1921 atgtaatcgg ggacattcag gccagtaaat caaacattac tattggtgac actacagcat
     1981 atattgattt gcatgctggt aaaaatatta ccggtgatgg ttttggcttc cgccagaata
     2041 ttgtgcgtgg aaactcacaa ggagaaacgc tgtttacagg agggatcaca gcagaagaca
     2101 gcactatcgt tattaaagat aaagcaaaag cattattttc aaattatgta tacctgctga
     2161 acacaaaagc aaccatagag aaaggtgctg atgtgacaac tcaaagtggt atgttctcca
     2221 cgagcgatat cagcgtctct ggtaatctgt ccatgacagg caatcccgac aaagacaata
     2281 aattcgagcc ctcaatatat ctgaatgatg cttcttatct actgactgac gactccgcca
     2341 gactcgttgc caaaaataaa gcatctgtgg tgggagatat acactccact aaaagtgcat
     2401 ccatcatgtt tggtcatgat gaaagcgacc tctcgcagtt gtctgacaga acctcaaaag
     2461 ggcttgcact tggtctttta ggtggctttg atgtctcata tcgcggttca gtcaatgccc
     2521 cgtcagcatc tgccactatg aacaacacct ggtggcaact aaccggagat tctgcgctga
     2581 aaacactgaa aagtacaaac agcatggtct atttcactga cagcgcaaac aataagaaat
     2641 tccatacgct gacggtcgat gaactggcaa ccagcaacag cgcctatgcg atgcgtacgg
     2701 acctgaaaaa ttcggataag ctggtagtaa acaaaaagct gtcaggtaag gacaatattc
     2761 tgttggttga ttttctgaac aaaccctccg gagaaaaact ggatattgaa ctggtgagtg
     2821 caccggggaa cagcagtaaa gatgtcttta aaggaagtga acaggcgata ggtttcagta
     2881 atgtaacgcc ggtcattaca acccgggaaa ccgatgacaa aataacatgg tcactgacag
     2941 gctataacac ggttgcaaac aaggaagcaa cccggaatgc cgccgccctg ttctctgttg
     3001 actataaagc gtttctgaac gaggtcaaca acctgaacaa acgtatgggt gacctgcgtg
     3061 acatcaacgg cgaagccggt gcatgggcac gcatcatgag cggtactggc tctgccagtg
     3121 gtggtttcag tgacaactac acgcacgttc aggtcggggt cgacaaaaaa catgagctgg
     3181 acggactgga tttgtttacc ggtttcactg tcacacacac tgacagcagt gcctccgccg
     3241 atgttttcag cggtaaaacg aagtctgtgg gggctggcct gtatgcttcc gccatgtttg
     3301 attccggtgc ctatatcgac ctgattggca agtatgttca ccatgataat gagtacactg
     3361 caacctttgc cggactcgga acccgtgatt acagcacgca ttcatggtat gccggtgcag
     3421 aagcgggcta tcgctatcat gtcactgagg atacctggat tgagccacag gctgagctgg
     3481 tttacggttc tgtatccggt aaacagtttg catggaagga ccagggaatg catctgtcca
     3541 tgaaggacaa ggactacaat ccgctgattg gccgaactgg tgtggatgtg ggtaaatcct
     3601 tctctggtaa ggactggaaa gtgacagccc gtgccggtct gggctaccag ttcgacctgc
     3661 tggttaacgg cgaaaccgta ttgcgggatg catctggtga aaaacgcatc aaaggtgaaa
     3721 aggacagccg tatgctgatg tccgttggcc tgaatgcaga aatcagggat aacgtccgct
     3781 ttggactgga gtttgagaaa tccgcctttg gtaagtacaa cgttgataat gcagtcaacg
     3841 ctaacttccg ttactcgttc tgatactccg tattcaaaag ggcctgaggg ccctttacgc
     3901 tcagtgcctg ccgccctgat atgtctgaga gacatttccg ggatgtcagt cgactggttt
     3961 acttaactgc gatgactaaa gctggcagta atgccggctt tttgtaagcg tcaacggagc
     4021 accgtattga cgcttattta ttggtgagta ctacgttcca tggcaggagt tcatcaactc
     4081 ggttggaagg ccattccggc agtacgctca ggatatggcg cagatacgct tccggatcga
     4141 taccgttcag actgcacgaa gcgctctttc cgcagcatta ttatccgcct ccgccagacc
     4201 gtcatcactg taataacaga gggcatccca ctgattcagt acatagctga acgcttcgcc
     4261 cagtctggat tttttcgaca gagtaccatt cttctccacc atccattcat gcgtaagcgc
     4321 atcatttaaa ccgtctttcg cctccctttc ctgtttccga tactaatgtc cattttcgca
     4381 gtaaaaggac atttaagatg aatgcacgaa aggcggtact ggcagataat ccggaattga
     4441 tcctgcgtgt gctacagctg agatttgacg agtcactgtc gtacccgcgc atttctgcgc
     4501 agaccggagt cagcaaaacc gccatttttt ctctggtgag gcgatttcac cagatattcg
     4561 ctgactggcc tctttccggt gaatattcct gcatgcaact ggcccgggct cttttccctg
     4621 ggcgataccc ttcagccccg acagtgactc agcctgtgaa aacagagaaa atccgccgga
     4681 accgattttc accggagttt aaatggaggc ttgttcagca aactctttta cccggtgcct
     4741 gtgtcgcaca aatagctcgc gagaacggaa tcaacgataa cctgctcttt aactggcggc
     4801 atctctggcg taacggcggc ctgcagccgc ccggcgaaca tgaaacatcg ctacttcccg
     4861 tgacgttaac gccggagccg gataataaaa tcccggcacc agtgcagata cctgaacaga
     4921 taaatacact gccagacagt ctgtgctgcg agctggttct gccggccgga actctcaggc
     4981 tgaaaggtga actgacaccg gcgttattac agacacttat ccgcgaaatg aaagggagca
     5041 gccactgatg atatctctcc ctgccggttc gcgtatctgg ctggttgccg gtatcaccga
     5101 tatgcgaaat ggctttaacg gcctggcctc aaaagttcag aacgtcctga aggatgaccc
     5161 gttctccggg catctgttca tcttccgcgg acgccggggt gaccagataa aagtgctgtg
     5221 ggctgacagt gacggactgt gcctcttcac caaacgcctg gagcggggcc gcttcgtctg
     5281 gtcggtcacc cgcgatggaa aggttcacct tactccggct cagttgtcca tgcttctcga
     5341 gggcatcgac tggaagcacc cgaaacgaac ggaacgcgct ggaatccgta tataacccgt
     5401 tgtaaagtga ggatatggac acctcacttg ctcatgagaa tgcccgcctg cgggcactgt
     5461 tgcagacgca acaggacacc atccgccaga tggccgaata caaccgcctg ctctcacagc
     5521 gggtggcggc ttatgcttcc gaaatcaacc ggctgaaggc gctggttgcg aaactgcagc
     5581 gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg cagatacagg
     5641 aagcacagga gctaatcagc gcacttcagg aagaaatggc agaaacgctg ggtgagcaat
     5701 atgacccggt actgccatcc cccctgcgcc agtcttcagc ccgtaaaccg ttaccggctt
     5761 cacttccccg tgaaacccgg gtcatccggc cggaagagga atgctgtccg gcctgtagtg
     5821 gtgaactcag tccactggga tgtgatgtgt cagagcaact ggagcttatc agcagtgcct
     5881 ttaaggttat cgaaacacaa cgtccgaaac aggcctgttg ccggtgcgac catatcgtgc
     5941 aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg cttctggccc
     6001 atgttgtcac cgggaaatat gcagaccatc tgccgttata ccgccagtca gaaatatacc
     6061 gtcgccaggg cgtggagctg agccgcgcca cgctggggcg ctggacaggt gccgttgctg
     6121 aactgctgga gccgctgtat gacgtcctgc gccagtatgt gctgatgccc ggtaaagtcc
     6181 atgccgatga tatccccgtc ccggtccagg agccgggcag tggtaaaaca aggactgccc
     6241 ggctgtgggt ctacgtccgt gatgaccgta acgccggttc acagatgcct ccggcggtct
     6301 ggttcgcgta cagcccggac cggaaaggcg tccatccaca gaatcatctg gccggttaca
     6361 gcggtgtgct tcaggccgat gcttacggtg gttaccgggt gttatacgaa tccggcagaa
     6421 taacggaagc cgcgtgtatg gctcatgccc ggagaaaaat ccacgatgtg catgcaagaa
     6481 tgcccaccga catcaccacg gaagccctgc agcgtatcgg tgaactgtat gccatagagg
     6541 cagaagtccg gggatgtaca gcagaacagc gtctggcggc aagaaaagcc agagctgcgc
     6601 cactgatgca gtcactgtat gactggatac agactcagat gaaaacactg tcgcgtcact
     6661 cggatacggc aaaagcgttc gcatacctgc tgaaacagtg ggatggcctg aacgtgtact
     6721 gcagtaatgg ctgggtggaa atcgacaaca acatcgcaga gaacgcctta cggggagtgg
     6781 ccgtaggccg gaaaaactgg ctcttcgcgg gttccgacag cggtggcgaa catgcggtgg
     6841 tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggaaccagaa aaatggctgc
     6901 gttacgtcat tgagcatatc caggactggc cggcaaaccg ggtacgcgat ctgttgccct
     6961 ggaaagttga tctgacctct cagtaaatat caatacggtt ctggcgagcc gcttacattc
     7021 atgcagcgac gtcagtaaca ctttgcttcg ctgctgcctg acggcaagac gctctgactc
     7081 cggtaatccc cgtatttcat cctcgatggc gtacagttca ctgattcgct tcagggcttc
     7141 ttctgccgtc gcacttttgc tgctgatgta tacatcgtgg atttttcgcc gggcatgggc
     7201 ccagcacgca acttctgtca gtgcaccacc ttcacgttct gcactgaaca acctgtcgta
     7261 acctgtgaac gcatccgcct gcaggatacc ccggaagggg cggaggtgtt gctccgggtg
     7321 tttcccctgc cggttcggcg agtacgcgaa ccagaccgct ggaggagatg acgaacccac
     7381 attgcgatca tcccggacat acgtccagat acgccctgtt ttcgcctttt tctgacccgg
     7441 tgccagtacc tttaccggtg tgtcatcagt gtgaaccttg cgggtgttca ttacataacg
     7501 gtacagggca tcattcaccg gtgtcattaa ctggcagtac gcgtcaaccc agttggagag
     7561 taaggcccgg ctcagttcga caccctggcg ggcaaagatt tcactctgac gatacagtgg
     7621 cagatgttcg cagtattttc ccgttaacac gcgggcaagt aatccggggc ccgcgatacc
     7681 acgctctatc gggcgggacg gcgccggtgc ttcaacaata cagtcacatt ttgtacaggc
     7741 tttttttacc cgttctgtgc ggatcacttt cagggcactg ctcaccagtt ccagctgttc
     7801 agcgctgact tcccccagat aatccagctc accgccacac tccggacaac agctttcttc
     7861 tggctccagg cggt
//
LOCUS       DAPGRN010000174         7737 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPGRN010000174 DAPGRN010000000
VERSION     DAPGRN010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.176"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(239..520)
                     /locus_tag="RHW33_004336"
     CDS             complement(239..520)
                     /locus_tag="RHW33_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV1647775.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            949..2535
                     /gene="pdeC"
                     /locus_tag="RHW33_004337"
     CDS             949..2535
                     /gene="pdeC"
                     /locus_tag="RHW33_004337"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV1647776.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCSPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKRDPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLIAHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARTNQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(2538..2861)
                     /gene="soxS"
                     /locus_tag="RHW33_004338"
     CDS             complement(2538..2861)
                     /gene="soxS"
                     /locus_tag="RHW33_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV1647777.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            2947..3411
                     /gene="soxR"
                     /locus_tag="RHW33_004339"
     CDS             2947..3411
                     /gene="soxR"
                     /locus_tag="RHW33_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV1647778.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSVRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            3957..5306
                     /gene="ghxP"
                     /locus_tag="RHW33_004340"
                     /gene_synonym="yjcD"
     CDS             3957..5306
                     /gene="ghxP"
                     /locus_tag="RHW33_004340"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV1647779.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            5457..7106
                     /locus_tag="RHW33_004341"
     CDS             5457..7106
                     /locus_tag="RHW33_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV1647780.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(7260..>7737)
                     /gene="espX5"
                     /locus_tag="RHW33_004342"
     CDS             complement(7260..>7737)
                     /gene="espX5"
                     /locus_tag="RHW33_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector pentapeptide repeat protein EspX5"
                     /protein_id="HDV1647781.1"
                     /translation="DLSFSKLDLINFEHADLSRVNLNKATLQNINLIDSKLFFTRLTN
                     TFLEMVICTGSNMANVNFNNANLSNCHFNCSVLTKAWMFNIRLYRVNFDEASVQGMGI
                     TILRGEENISINSDILVTLQKFFEEDCTSHTDMSQTEDNIHTVAMKITADIMQDAD"
BASE COUNT         1806 a         1769 c         2018 g         2144 t
ORIGIN      
        1 cggtgtcgtt aggtagtgca atttatttct ttttatccta gccttactca tatttttgaa
       61 ctatcaaaaa ttgctcattt gtataaaatt cagcaaggag gtggcgaaga tagtgaagat
      121 gctaagcgta caacaccttc aagcgttgat taaaagaaag tgcgatatgt gatgataggg
      181 cggccgagga agctattcat cgaccgcttg tgaagggata aaagtcaaga aagtgaaatc
      241 aggtcaaaat cattggccat tgtggcggtg tctgattcat ctcttctatc atcaccgact
      301 cgatgttgtg ccagatggcg gtgatatccg ccattgtgat gccaagcaac cccatcgcga
      361 accagcaggc ggaaaccacg cccagaccgc tgctgatcac tgcaagtccg acataatcag
      421 accgacgaaa acggatgttg agcgtgctgg ccagaaacat cattacggca ctaagaagtt
      481 gccagcgaag aagaaccacg ccagtggtga gggtagccat caaacaattc ctctgaaaag
      541 agccgatgcc ctggacagcg cggcgttgtt cacgggaggc gtggtacact ctggctatcg
      601 cggggcttgc agaacacaaa aatgaaacac tctgtttgtt tcattaattt tgtgaactat
      661 atcacaattg attgtttgtt agccatatta ggccgtgact tttattgctg tacagattat
      721 gtggtttttc agtggcatta agggtatatc ttcccgccgc ctctgtattc ctgtagaaaa
      781 ttaattttga atatcaatga attattttca tccaggtgac gattaaaaag gtatcaattt
      841 caaatcaggc aaaagtgcta tttataccgt aagatttatc taaagacgtc ggtagccagg
      901 gtttcacctt gcaatggccg ggtataaaca ggcaggaaat tgatagcaat gagtcatcgt
      961 gcacgacacc aattactggc gttgccgggc attatctttt tagttctctt tcccatcatt
     1021 ctttcgctat ggattgcctt cctttgggca aaatcagaag tgaataatca gctccgaacc
     1081 tttgctcaac tggcactgga taaatccgag ctggtcattc gccaggcaga tttagtgagc
     1141 gatgcagcag aacgctatca ggggcaagtt tgctctccag cccatcaaaa gcgaatgtta
     1201 aatattattc gtggctatct ttatattaat gaattgatct atgcccgtga taaccatttt
     1261 ttatgctcat cgctgatagc gcctgtaaac ggctatacga ttgcaccggc cgattataag
     1321 cgtgacccta acgtttctat ctattattac cgcgatacgc cttttttctc tggctataaa
     1381 atgacctata tgcagcgggg aaattatgtg gcggttatca accctctctt ctggagtgaa
     1441 gtgatgtctg atgacccgac attgcaatgg ggtgtgtatg atacggtgac gaaaaccttt
     1501 ttctcgttaa gcaacgaggc ctcggcagca acgttttctc cgctgattca tttgaaggat
     1561 ttaaccgtac aaagaaatgg ctatttatat gcgacagttt attcgacaaa acgcccaatt
     1621 gcagccattg ttgcgacttc atatcaacgt cttatagccc atttttataa tcatcttatt
     1681 tttgcgttac ccgccggtat tttggggagt cttgttctgc tattactctg gctacgtatt
     1741 cgacaaaact atttgtctcc caaacgcaaa ttgcaacgcg ccctagaaaa acatcaactt
     1801 tgtctttatt accagccaat aatcgatatc aaaacagaaa aatgtatcgg cgctgaagcg
     1861 ttgttacgtt ggcctggtga gcaggggcaa gtaatgaatc cggcagaatt tattccgctg
     1921 gcagaaaagg aggggatgat cgaacaggta actgattatg ttattgataa tgtcttccgc
     1981 gatctgggcg cgtacctggc aacacatgcc gatcgctatg tttctattaa cctgtcggcc
     2041 tccgattttc atacgtcacg gttgatagcg cgaaccaatc agaaaacaga gcaatacgcg
     2101 gtgcgtccac agcaaattaa atttgaagtg actgagcatg catttcttga tgtcgaaaaa
     2161 atgacgccga ttattctggc tttccgccag gccggttacg aagtggcaat tgatgatttt
     2221 ggtattggct actctaactt gcataacctt aaatcattga atgtcgatat tttgaaaatc
     2281 gataaatcgt ttgttgagac gctgaccacc cataaaacca gtcatttgat tgctgaacat
     2341 atcatcgagc tggcgcacag cctggggtta aaaacgatcg ctgaaggcgt cgaaactgag
     2401 gagcaggtta actggctgcg caaacgcggt gtgcgctatt gccagggatg gttctttgcg
     2461 aaggcgatgc cgccgcaggt gtttatgcaa tggatggagc aattacccgc gcgggagtta
     2521 acgcgcgggc aataaaacta caggcggtgg cgataatcgc tgggagtgcg atcaaactgc
     2581 cgacggaaaa cgcgggagaa ggtctgctgc gagacataac ccaggtccat tgcgatatca
     2641 aaaatcggac gctcggtggt gcgcaactca acggcggcca gtaacaggcg gcgttggcga
     2701 atgtaatcgc caagcgtctg atgcgtcacc gtgcggaaca tccgttgcag gtaccacttt
     2761 gaatagcctg atttttttgc gacgacatca atgttaagcg gctggtcaat atgttcgtca
     2821 atccatgcaa taagatcctg aataattttc tgatgggaca taaatctgcc tcttttcagt
     2881 gttcagttcg ttaattcatc tgttggggag tataattcct caagttaact tgaggtaaag
     2941 cgatttatgg aaaagaaatt accccgcatt aaagcgctgc taacccccgg cgaagtggcg
     3001 aaacgcagcg gtgtggcggt atcggcgctg catttctatg aaagcaaagg gttgattacc
     3061 agtgtccgta atagcggcaa tcagcgccga tataaacgtg atgtgttgcg atatgtcgca
     3121 attatcaaaa ttgctcagcg tattggcatt ccgctggcga ccattggtga agcgtttggc
     3181 gtgttgcccg aagggcatac gttaagtgcg aaagagtgga aacagctttc gtcccaatgg
     3241 cgagaagagt tggatcggcg cattcatacc ttagtggcgc tgcgtgacga actggatgga
     3301 tgtattggtt gtggctgcct ttcgcgcagt gattgcccgt tgcgtaaccc gggcgaccgc
     3361 ttaggagaag aaggtaccgg cgcacgctta ctggaagatg aacaaaacta aagcgccaca
     3421 agggcgcttt agtttgtttt ccggtctttg tctttctctc tatcccgctg gtacacagga
     3481 gggtttcccc cgacgtcaac acacctcatt cgagcacgtg gtggaggttc cggttggtgt
     3541 tgatgcttta attgtatgtc accgacgttt cttcgccagt gtaaaagtat actttttaac
     3601 cgcaatattt ttgtcatctc agacgatttt ttatcgcaat cctgaacggt atacggctcg
     3661 ataacgctgc aatcttgcgc accgacgata acgtttgcgc atcaattgcc tggtttttca
     3721 tcgtcaagac aataaaagag aaaaaagcag caaacttcgg ttgaaaaagc cgctatgatc
     3781 gccggataat cgtttgcttt ttttaccacc cgttttgtat gcgcggagct aaacgtttgc
     3841 ttttttgcga cgcagcaaat tgtcgcaaac ctggagcagg aagataacgt ttcgctggca
     3901 ggggattgtc cgccacgcat cttgacgaaa attaaattct caggggatgt tttcttatgt
     3961 ctacgccatc agcgcgtacc ggcggttcac tcgacgcctg gtttaaaatt tcacaacgtg
     4021 gaagcactgt ccgtcaggaa gtggttgccg ggttaacaac gtttctggcg atggtctact
     4081 cggttatcgt cgttccaggc atgttgggta aagcgggctt cccgcctgcg gcagttttcg
     4141 ttgcaacctg tctggttgcc ggactcggtt ctatcgtgat gggtctgtgg gctaatctgc
     4201 cgttggcgat tggttgcgcc atctccctga cggcgtttac cgcattcagc ctggtgctgg
     4261 ggcaacatat tagcgtacct gtcgcgctgg gtgccgtgtt cctgatgggt gtgctgttta
     4321 cggtaatttc tgccacgggt atccgtagct ggattttgcg caacttgccg cacggtgtgg
     4381 cgcacggcac ggggatcggt atcggtctgt tcctgctgct cattgccgct aatggtgtcg
     4441 gtctggtgat taaaaacccg cttgatggtc tgcccgttgc gctgggtgat ttcgcgacct
     4501 tcccggtgat tatgtcactg gtaggtctgg cggtgatcat cggcctggaa aaactgaaag
     4561 tccctggtgg cattctgctg accattatcg gtatctcaat tgtcggtttg atcttcgatc
     4621 ctaacgtcca tttctccggc gttttcgcca tgccttcatt gagcgatgaa aacggcaatt
     4681 cactgattgg cagcctggac attatgggcg cgctgaatcc tgtagtcctg ccaagcgttc
     4741 tggcgctggt gatgacggca gtatttgatg ccaccggaac tatccgcgct gtcgccggcc
     4801 aggcgaacct gctggataaa gatgggcaga tcatcgacgg cgggaaagca ctgaccactg
     4861 actccatgag cagcgttttc tctggcctgg tgggtgcagc tccggcagcg gtatacatcg
     4921 agtctgcggc gggtacggcg gcgggcggta aaaccgggtt gacggctatc accgttggcg
     4981 tgctgttcct gctgattctg ttcctctctc cgctttctta cctcgttccg gggtatgcaa
     5041 cggctccggc gctgatgtac gttggcctgc tgatgctgag caacgtggcg aaaatcgact
     5101 ttgctgattt tgttgatgcg atggcgggtc tggttacggc ggtattcatc gtgctgacct
     5161 gtaacatcgt aacaggcatc atgatcggct tcgcgactct ggtgattggt cgtctggttt
     5221 ccggtgaatg gcgcaagttg aacatcggta cggtcgttat cgccgtggcg ctggtgacct
     5281 tctatgcggg tggctgggct atctaatctt tcatcggatt ctgaaaacgg gtggcaatgg
     5341 ctgcccgttt ttattttctc cgcacattgt gtgagctttt gcgatattct gaaaaaatga
     5401 gaattcaggc ataacgtctt tctcagatca taaaaaacat cgcaaacagg gaacgcatgg
     5461 aaatcttctt caccatactg ataatgaccc tcgtggtctc gctgtccggg gtggtcactc
     5521 gtgtcatgcc ctttcagatc ccgcttccgc ttatgcaaat cgccatcggt gcgctactgg
     5581 cgtggccgac gtttggtttg catgtggagt ttgatcctga actcttttta gtcttgttta
     5641 tcccgccgtt gctgttcgct gatggctgga aaacgccgac ccgtgaattt ctcgaacatg
     5701 gtcgagagat tttcggcctc gcactggcgc tggtggtggt caccgtggtc ggcattggct
     5761 tccttattta ctgggtggtg ccgggcattc cgctgatccc cgcctttgcg ctggcggcgg
     5821 tactttctcc gaccgatgct gtggcgctct ccgggattgt tggcgaaggg cgcatcccga
     5881 aaaaaatcat gggcattttg cagggcgaag cgttgatgaa tgacgcctcc ggtctggtgt
     5941 cgttgaagtt tgccgtagca gtggcgatgg ggacgatgat cttcaccgtc ggcggtgcga
     6001 cggtcgaatt tatgaaagta gccattggcg gtattcttgc cggttttgtt gtgagctggc
     6061 tgtatggtcg ctcgctgcga ttcctcagcc gctggggcgg tgatgaaccc gcgacgcaga
     6121 ttgttctgct gttcttgctg ccattcgctt cttatctgat tgccgaacat attggcgttt
     6181 ccggcatcct cgctgcggtt gccgccggga tgaccatcac ccgctccggt gtgatgcgcc
     6241 gtgcgccact ggcaatgcgc ctgcgcgcaa acagcacttg ggcgatgctg gaatttgttt
     6301 ttaacggcat ggtgttcctg ctgttaggtc tgcagctacc gggtattctg gagacgtcgc
     6361 tgatggcggc agaaatcgac cctaacgtcg aaatctggat gctgtttacc gatattattc
     6421 tgatatatgc ggcgctgatg ctggtccgtt tcggctggct gtggacgatg aaaaagttca
     6481 gcaaccgctt cctgaagaag aagccgatgg agtttggttc gtggaccaca cgagaaatcc
     6541 tgatcgcgtc tttcgccggg gtgcgtgggg cgatcactct ggccggtgtg ctctctatcc
     6601 cgctgctctt gccggatggt aacgtcttcc cggcgcgcta tgagctggtg tttctggcgg
     6661 ctggtgtcat tctcttctcg ctgtttgtcg gcgtggtgat gttgcctatt ctgctacaac
     6721 acattgaagt cgcggaccat tcgcaacaat tgaaagagga acgtattgcg cgagcggcaa
     6781 cggcagaagt ggcgattgtg gcgatccaga aaatggagga gcgtctggcg gcggataccg
     6841 aagagaatat cgataaccag ctgcttacgg aggtcagttc tcgcgtcatt ggtaacctgc
     6901 gtcgtcgcgc cgatggacgt aacgacgttg aaagttctgt gcaggaagag aaccttgagc
     6961 gtcgcttccg tctggcggca ttgcgttctg aacgtgctga actgtaccac ctgcgcgcca
     7021 cgcgggagat cagcaacgaa acgctgcaaa aattactgca cgatctcgat ttgcttgaag
     7081 cgttgttgat tgaggaaaat cagtaagccg aaagcgacgt aaccaggccc gagctaacct
     7141 gttaaaggtg caatcttttg cacctttaac aggttagctc gggctaacat tcatgattct
     7201 aaaacaaata agttatatct cttttaaagt caattcattg gctgttcttc gacttaacct
     7261 caatctgcat cttgcataat atctgcagta atcttcatag cgactgtatg aatattatcc
     7321 tcagtttgtg acatgtcagt atgagaggta caatcttctt caaagaattt ctgtagtgtt
     7381 accaggatat cactattaat ggagatattt tcctcaccac ggagaatggt aatacccatt
     7441 ccctggacgc tagcctcatc gaaattaaca cgatagagac ggatattaaa catccaggct
     7501 tttgttaaaa cagaacagtt gaaatggcaa ttgcttaaat tggcattatt aaaattaaca
     7561 ttagccatat tagaaccggt acatataacc atttcgagga acgtatttgt taaccgcgta
     7621 aaaaagagtt tgctatcaat taagtttata ttctggaggg ttgctttatt caggttgaca
     7681 cgagacagat cagcatgttc aaagttaatt aaatcaagtt tagagaagct aaggtcg
//
LOCUS       DAPGRN010000175         7591 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPGRN010000175 DAPGRN010000000
VERSION     DAPGRN010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.177"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1156
                     /gene="chbC"
                     /locus_tag="RHW33_004343"
     CDS             <1..1156
                     /gene="chbC"
                     /locus_tag="RHW33_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV1647782.1"
                     /translation="DASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALAEERKV
                     DALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTFIVRRN
                     WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLAS
                     LGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVEAALAA
                     GKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEP
                     ILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFNT
                     NGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            1207..1557
                     /gene="chbA"
                     /locus_tag="RHW33_004344"
     CDS             1207..1557
                     /gene="chbA"
                     /locus_tag="RHW33_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV1647783.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1565..2407
                     /gene="chbR"
                     /locus_tag="RHW33_004345"
     CDS             1565..2407
                     /gene="chbR"
                     /locus_tag="RHW33_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV1647784.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            2512..3864
                     /gene="celF"
                     /locus_tag="RHW33_004346"
                     /gene_synonym="chbF"
     CDS             2512..3864
                     /gene="celF"
                     /locus_tag="RHW33_004346"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV1647785.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            3877..4635
                     /gene="chbG"
                     /locus_tag="RHW33_004347"
     CDS             3877..4635
                     /gene="chbG"
                     /locus_tag="RHW33_004347"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV1647786.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEVLFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(4682..6943)
                     /gene="katE"
                     /locus_tag="RHW33_004348"
     CDS             complement(4682..6943)
                     /gene="katE"
                     /locus_tag="RHW33_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV1647787.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILCEKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            7126..7389
                     /gene="cedA"
                     /locus_tag="RHW33_004349"
     CDS             7126..7389
                     /gene="cedA"
                     /locus_tag="RHW33_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV1647788.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
BASE COUNT         1869 a         1700 c         1988 g         2034 t
ORIGIN      
        1 cgacgcctca accattgaaa cacttaatgg tctgaaaggt attggcggca acgtatataa
       61 cggaacatta gggataatgt ctttaatggc accgttcttt attggcatgg cgctggcaga
      121 agagcgtaaa gtcgatgcgc tggcggctgg gttgttatcc gttgcagcat ttatgaccgt
      181 caccccatat agtgtcggtg aggcctatgc ggttggcgca aactggttag gtggggcgaa
      241 tatcatctcc gggattatta ttggcctggt ggtggcagaa atgtttacct ttattgtccg
      301 ccgcaattgg gtcattaaac tgcccgacag cgtacctgct tcagtatcgc gttccttctc
      361 ggcattaatt cccggcttta ttattctttc cgtgatgggg attattgcct gggcgttgaa
      421 tacctggggc accaacttcc atcagatcat tatggatacc atctcaaccc cactggcatc
      481 gttgggtagc gtggtgggct gggcctatgt gatctttgtt ccactgctct ggttcttcgg
      541 tattcatggc gcgctggcgc tgaccgcact ggacaacggc attatgacgc cgtgggcgct
      601 ggaaaatatc gcgacctatc agcaatatgg ttccgtcgaa gcggcgctgg cagccggtaa
      661 gaccttccat atctgggcca agccgatgct ggactccttt attttccttg ggggcagtgg
      721 tgcgacttta ggcctgatcc tggctatctt tatcgcctct cgccgtgctg attatcgtca
      781 ggtggcaaaa ctggcgctgc cgtccggcat cttccagatt aacgaaccga ttctgtttgg
      841 tctgccaatt atcatgaacc cggtgatgtt tatcccgttt gtactggtac aaccgattct
      901 ggcggcaatc accctcgcag cgtactacat gggcattatt cctccggtga ccaatattgc
      961 accgtggacc atgccaaccg gtctgggagc cttctttaat accaacggta gcgtcgccgc
     1021 attgctggtc gcactcttca accttggcat cgcaacgtta atttatctgc cctttgttgt
     1081 ggtggctaac aaagcacaaa atgcgattga taaagaagag agcgaagaag atatcgctaa
     1141 cgccctgaaa ttttaatcga tgccggtacg ggtaatcgtg ccggtaagaa aagagaggaa
     1201 cgatgtatga tggatctcga taacattccc gatacgcaaa cggaagctga agagctggaa
     1261 gaagtggtga tggggcttat catcaactcc ggacaagcgc gcagcctggc gtatgcggca
     1321 ctgaaacagg cgaagcaggg cgattttgcc gcagcaaaag ccatgatgga tcagtcacgg
     1381 atggcattga atgaagcgca tctggtacag acgaaactga ttgaaggcga tgcgggcgaa
     1441 ggtaagatga aagtgagtct ggtgctggtc cacgctcagg atcatttaat gacgtccatg
     1501 cttgcgcgtg aactgattac tgaattaatt gagcttcatg aaaaactgaa ggcataagga
     1561 gtcgatgatg cagccagtga ttaacgcgcc ggaaattgcc actgcccgag aacagcagtt
     1621 gtttaatggc aaaaacttcc atgtgtttat ctataacaaa actgagagta tcagcggact
     1681 gcatcagcac gactattatg aatttactct ggtattaacc gggcgttatt tccaggagat
     1741 taacggtaag cgcgtgttac tggaacgggg cgattttgtt tttattccgt taggttcgca
     1801 ccatcaaagt ttttatgagt ttggtgccac gcgtatattg aacgttggga tcagtaaacg
     1861 cttttttgag cagcattacc tgccgttgtt gccttattgc tttgtagctt cgcaggtata
     1921 ccggaccaat aacgcttttc tcacctatgt ggaaacagtg atttcttcat tgaatttccg
     1981 cgaaacaggg ctggaagagt ttgttgagat ggttactttt tatgtcatta accgtttacg
     2041 tcattaccgc gaagaacagg tgattgatga tgtaccgcag tggctgaaaa gtacggtaga
     2101 aaagatgcat gataaagagc agtttagtga atcggcgctg gagaatatgg tgacgttgtc
     2161 agccaaatca caggaatatt tgacgcgggc gactcaacga tattatggca aaacgccaat
     2221 gcagattatt aatgaaatcc gtattaattt tgccaaaaaa caactggaaa tgaccaacta
     2281 ttcagtgacg gatattgcgt ttgaggccgg ttatagtagc ccgagtttgt ttattaaaac
     2341 gtttaagaaa ttaacgtcct ttacgcctaa gagctatcgt aagaaattga ctgaatttaa
     2401 tcagtaagtc gttatacctg acaattcaca tatctgtcct gttgctggat tatttatgtc
     2461 cgggggggca gatatgccag atatcagtat tctgtactga agggagaaat tatgagccag
     2521 aaattaaaag tcgtcactat tggtggcggg agcagctata ccccggagtt actggaagga
     2581 tttattaagc gttatcacga attgccggtc agcgaattat ggctggtgga tgtcgaaggt
     2641 ggcaaagcga aactggatat tattttcgat ctctgccaac ggatgattga taacgctggc
     2701 gtcccgatga agctttataa aacgctggat cgccgcgaag cattgaaaga tgctgatttc
     2761 gttactaccc aactgcgcgt aggccaatta ccggcgcgcg aactggatga acgtattcca
     2821 ttaagtcatg gttatcttgg tcaggaaacc aacggcgcgg gcggtctgtt taaaggtctg
     2881 cgtaccattc cggtgatttt tgacatcgta aaagatgtcg aagaactttg tccgaatgca
     2941 tgggtgatta acttcactaa cccggcggga atggtcactg aagccgttta tcgtcatacc
     3001 ggatttaaac gctttatcgg cgtgtgtaat attccgatcg gcatgaagat gtttattcgc
     3061 gatgttctga tgctgaaaga cagcgatgat ttatctatcg atctgttcgg cctcaaccat
     3121 atggtgttca ttaaggatgt gctggtaaat ggcaaatctc gctttgccga attacttgat
     3181 ggtgtggcat ccgggcagtt aaaagcatct ggcgttaaaa atattttcga tctgccattt
     3241 agcgaaggct taattcgttc tctgaatctg ttgccgtgtt cttatttgct ttattacttc
     3301 aagcaaaaag agatgctggc tattgaaatg ggcgaatact acaaaggcgg cgcacgagcg
     3361 caggtagtac agaaagtcga gaaacaactt tttgagctgt ataaaaaccc ggagttgaaa
     3421 gttaagccga aagaactgga acagcgcggt ggggcttatt actctgatgc agcgtgcgaa
     3481 gtgatcaacg ctatctacaa cgacaagcaa gcagaacatt acgttaatat cccgcatcat
     3541 gggcatattg ataatattcc ggcagactgg gcggtagaaa tgacctgtac gctggggcgc
     3601 gatggcgcga cgccacatcc gcgcattacg catttcgatg ataaagtgat ggggctgatt
     3661 cacaccatta aaggcttcga gattgctgcc agcaacgccg cacttagcgg agagtttaac
     3721 gatgtgttac tggcgctaaa ccttagtccg ttggtgcatt ccgatcgcga tgctgagctg
     3781 ctggcacgcg agatgattct ggcgcacgag aaatggctgc caaattttgc cgactgcatc
     3841 gcagagctta aaaaagcaca ttaaccgagg ctgattatgg aacgcttact gattgttaat
     3901 gccgatgatt ttggcttaag caaaggtcag aactacggca ttatcgaggc ctgtcgcaat
     3961 gggattgtca catcgacgac ggcgctggtg aatgggcagg ctattgacca tgcggtgcag
     4021 ttgagtcgtg atgaaccgag tctggccata gggatgcact ttgtccttac tatgggcaag
     4081 ccactgacag ctatgccggg gttaacccgc gatggtgtgc tgggaaaatg gatctggcag
     4141 ttggcagaag aagatgcttt accgctggaa gaaattactc aggagcttgc cagtcagtat
     4201 ttgcgtttca ttgagctatt tggacgcaaa cctacgcatc ttgatagcca tcatcatgtg
     4261 catatgttcc cgcagatttt ccctattgtt gccaggtttg cggctgaaga ggggattgcg
     4321 ttgcgcatcg accgtcagcc gctatctaac gctggtgatt taccggctaa tctgcgcagt
     4381 tcgcacggat tcagtagcgc gttttatggt gaagagatta gcgaggtact gttcctgcaa
     4441 gtgcttgatg attccagcca tcgcggggaa cgctcgctgg aagtcatgtg ccatccggcg
     4501 tttgtcgata atacgattcg ccagagtgct tactgtttcc cacggttaac ggagctggaa
     4561 gtgctgacat cagcgtcgtt gaaatatgca attgctgagc gtggttatcg gttgggtagt
     4621 tatcttgatg tgtaagtaag gtggtaaggt cactccccag gggacgtgac cttatccata
     4681 tttacgccgg aattttgtca atcttaggaa tgcgtgacca cacgcggtgt gctgccatca
     4741 gcgttaacag ttcatccata aaactaccgt cggcgctgtc agcttccaca atcccttctt
     4801 caccctggtc agcgaccttg attgttgctt taaacttgcg cgcgtctccc gccagcgcaa
     4861 tcggtttaag gtgtttgtag gcttccatca ggtagtagtt ggcatcgccg ttgtcagcga
     4921 tatccgcgat attgccgcaa gggacaatga ccgcatcgac cgtcagcgaa ggcgcaccgg
     4981 caaaggtagc ggctataggc aacaccgtac cgtcatccgc agtcacttca cccattcggg
     5041 agtagagcag tttggcatga acgcctttgg ccttcagggc cttgagaatg gccagaaggt
     5101 ctgccgatct cacttcatca ttaagtaaaa tcgctaccac gcgacctttc acatcaccgt
     5161 caggaatggc gtacaaactt aaggatggat cctttttcag accgttgacg tccggaggtg
     5221 gggtgatatt cagctggtcg tcagtcagtt cgataccgag atttttcgcc accgcctggg
     5281 ccagagtgag atcaatatgc gccagctggt caacaacgcg ctcacgaata tacggacgaa
     5341 cgactttgct taactcaaaa ctgaaaccat cgacaatatg acgctgctcg aatggcgtct
     5401 gacttagcca gaacagacgc ggatgggaat aatattcgcc aaaagatgga ctgcgctcgc
     5461 gaactttatt tccttccacg cgttcctgat atgattcaaa accgccgcgt ttcggtcccg
     5521 gcggtgtttc gcgcggccag ttatcgttga tcgagttcgg ttcgtaattc gccgggttag
     5581 tgtcgatccc catacgatgc atgccatcac gctggaagtt gtggtaaggg caggtcggac
     5641 ggttaatcgg aatctcatgg aaattcggcc caccaagacg actgatttgt gtatcggtat
     5701 aggagaacaa acgtccctgc aacagcggat cgttggtgaa gtccagtccc ggcacgatat
     5761 gcccaggatg gaaagccgcc tgttcgtttt cagcaaagaa gttatccggg ttgcgattga
     5821 gcaccatttt gccgacacgc tgaacgggca ccagttcttc cgggataagt ttggttggat
     5881 cgagaagatc gaagtcgaac ttgaattcat cttcttcagg aatcaactgg aagcccagtt
     5941 cgtattccgg aaaatcgcct gcttcaatgg cttcccacaa ctcgcggcgg tggaagtccg
     6001 ggtcacgtcc ggttagtttt tgtgcttcat cccaaacgag tgaggcttta cctgccagtg
     6061 gtttccagtg gaaacgtaca aacgttgcct tcccttcggc attaatcagg cggaaggtat
     6121 gaataccaaa gccttccatg gtgcggtaac tgcgggggat gccgcgatcc gacatcgccc
     6181 acatcacgtt gtgcagagtt tcaggttgca gagaaacata atcccagaaa gtatcgtggg
     6241 cgctttgccc ttgtggaatt gcccagtgtg gttctggttt taccgcatga acaaaatcgg
     6301 ggaatttatg cgcatcctgg ataaagaaga ttggcgtgtt attgccaaca aggtcaaaaa
     6361 taccctcttc ggtatagaat ttggtggcaa agccacggat atcacgcacg gtatcggcag
     6421 agccagcacc accctgaacg gtagagaacc taacaaatac tggggtgatt ttgttcggat
     6481 ctgagaggaa atccgctttg gtgatatcgc ttaagctttt atatggctgg aaataaccgt
     6541 gagctgctga tccacgtgca tgaacaatac gttccgggat gcgctcatgg tcaaagtggg
     6601 tgattttctc gcacagaata aaatcttcca gcagcgttgg accacggcta ccggcacgca
     6661 gtgagttttg atcgtcggcg atgcgcacgc cctgattagt ggtcagcgca taattttcac
     6721 tgcctttgcg tacgtcttcc agagaattaa gtttttcgtt acgcgtatca ggggctttca
     6781 ggctccctgg ggcggtaggt tgtgcacctg gcggtgttgg ttcagccgct ggacgatgag
     6841 agccgtcctc aggtgccagt gagtccatcc ccggtttcgc ttcgctggaa tcgtgtagtg
     6901 gtgactggtg ctgatgtggg ttcttttcgt tatgttgcga cattgaactc gtctccttaa
     6961 tttattactg aaagggccgc tgttttggtc aaaaaactct ctaactatag accaccagcc
     7021 agatcccact tcggagacta ttctggaaac ggtgttatga aatacacgca aagcgggacg
     7081 tacaggggct aaatcggcta aactacagtt tttctgattt ttaatatgcg tttagtgaag
     7141 ccagtaatga agaaaccgct ccgccagcaa aaccgccaga ttattagcta tgtcccacgc
     7201 acggaacccg cgccgccaga acatgcgata aagatggatt cgtttcgtga tgtctggatg
     7261 ctgcgtggca aatatgttgc gtttgtactg atgggagagt catttctgcg ctcaccggcg
     7321 tttactgtgc ctgaatccgc ccagcgttgg gcaaatcaga tccgccagga aggggaagtg
     7381 actgagtaag gattacgtac ctcccggttt tgccgggagt tacatggcct gaaattttgt
     7441 atttcattgc tacattcatt caaaccacct gtaacaaaaa gctgcctttg acgatctatt
     7501 gctataaata agtgatcttt tttctttaat tgtttattta ttttaaaatg gttaagtttt
     7561 gctaatcatg atgagctatt tttgatagtt c
//
LOCUS       DAPGRN010000176         7586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPGRN010000176 DAPGRN010000000
VERSION     DAPGRN010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.178"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            251..4504
                     /gene="fdeC"
                     /locus_tag="RHW33_004350"
                     /pseudo
     CDS             251..4504
                     /gene="fdeC"
                     /locus_tag="RHW33_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            complement(4625..5482)
                     /locus_tag="RHW33_004351"
     CDS             complement(4625..5482)
                     /locus_tag="RHW33_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1647789.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNVNFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5730..6599
                     /locus_tag="RHW33_004352"
     CDS             5730..6599
                     /locus_tag="RHW33_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1647790.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTREELFLTSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6759..7352)
                     /gene="rclC"
                     /locus_tag="RHW33_004353"
     CDS             complement(6759..7352)
                     /gene="rclC"
                     /locus_tag="RHW33_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV1647791.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSITIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7364..>7586)
                     /locus_tag="RHW33_004354"
     CDS             complement(7364..>7586)
                     /locus_tag="RHW33_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV1647792.1"
                     /translation="VLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIAD
                     IARKYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2078 a         1692 c         1946 g         1870 t
ORIGIN      
        1 aagtatctat ttaatgactt gcacaaaaag ctaaattttc acccataaat aaaaatataa
       61 tcccgcgccc aaccacctga tgagtggcta cagacactgg atatattagg tggcggtgca
      121 ctttcttaca taaaggtatt tccttttctg tggaaaagga aatcgggaaa tccccggttt
      181 ttctgacaag cagacgccat tatttgtgtc tgcctatgtt cgttaattcg ttcatcagga
      241 aattatctca atgtcacgtt ataaaacagg tcataaacaa ccacgatttc gttattcagt
      301 tctggcccgc tgcgtggcgt gggcaaatat ctctgttcag gttctttttc cactcgctgt
      361 cacctttacc ccagtaatgg cggcacgtgc gcagcatgcg gttcagccac ggttgagcat
      421 gggaaatact acggtaactg ctgataataa tgtggagaaa aatgtcgcgt cgtttgccgc
      481 aaatgccggg acatttttaa gcagtcagcc agatagcgat gcgacacgta actttattac
      541 cggaatggcc accgctaaag ctaaccagga aatacaggag tggctcggga aatatggtac
      601 agcgcgcgtc aaactgaatg tcgataaaga tttctcgctg aaggattctt cgctggaaat
      661 gctttatccg atttatgata cgccgacaaa tatgttgttc actcaggggg caatacatcg
      721 tacagacgat cgtacttagt caaatattgg ttttggctgg cgtcattttt caggaaatga
      781 ctggatggcg ggggtgaaca cctttatcga ccatgattta tcccgtagtc atacccgcat
      841 tggtgttggt gcggaatact ggcgcgatta tctgaaactg agcgccaatg gttatattcg
      901 ggcttctggc tggaaaaaat cgccggatat tgaggattat caggaacgcc cggcgaatgg
      961 ttgggatatc cgcgcagagg gctatttacc tgcctggccg cagcttggcg caagcctgat
     1021 gtatgagcag tattatggcg atgaagtcgg gctgtttggt aaagataagc gccagaaaga
     1081 cccgcatgcg atttctgccg aggtgaccta tacgccagtt cctcttctga cactgagcgc
     1141 cgggcataag cagggcaaga gcggtgagaa tgacactcgc tttggcctgg aagttaacta
     1201 ccgaattggc gaacctttgg cgaaacaact cgatacggat agcattcgcg agcgtcgggt
     1261 actggcaggc agccgctatg acctggttga gcgtaataac aacatcgttc ttgagtaccg
     1321 caaatctgaa gtgatccgta ttgctctgcc tgagcgtatt gaaggtaagg gcggtcagac
     1381 actttccctg gggcttgtgg tcagcaaagc aactcacgga ctgaaaaatg tgcagtggga
     1441 agcgccgtca ttactggctg aaggtggcaa aattaccggt cagggtagtc agtggcaagt
     1501 aacgctcccg gcttatcgtc caggcaaaga caattattat gcgatttctg cggttgccta
     1561 cgataacaaa ggcaatacct caaaacgcgt gcagacagag gtggtcatta ccggagctgg
     1621 tatgagcgcc gatcgcacgg cgttaacgct tgacggtcag acccgtattc aaatgcttgc
     1681 taacggtaat gagcaaaaaa cgctggtgct gtctctgcgc gacgccgagg gccagccagt
     1741 cacgggcatg aaagatcaga tcaagactga actaactttc aaaccggctg gaaatattgt
     1801 gactcgttcc ctgaaggcca ctaaatcaca ggcaaagcca acactgggtg agttcaccga
     1861 aactgaagca ggggtgtatc agtctgtctt tactaccgga acgcagtcag gtgaggcaac
     1921 gattactgtt agcgttgatg gcatgagcaa aaccgtcact gcagaactgc gggccacgat
     1981 gatggatgtg gcaaactcca ccctgagcgc taacgagccg tcaggtgacg tggttgctga
     2041 tggtcagcaa gcctatacgt tgacgttgac tgcggtggac tccgagggta atccggtgac
     2101 gggagaagcc agccgcttgc gatttgttcc gcaagacact aatggtgtaa ccgttggtgc
     2161 catttcggaa ataaaaccag gcgtttacag cgccacggtt tcttcgaccc gtgccggaaa
     2221 cgttgttgtg cgtgctttca gcgagcagta tcagctgggc acattacaac aaacgctgaa
     2281 gtttgttgcc gggccgcttg atgcagcaca ttcgtccatc accctgaatc ctgataaacc
     2341 ggtggttggg ggtacagtta cggcaatctg gacggtaaaa gatgcctatg acaaccctgt
     2401 gaccagcctc acgccggaag cgccgtcatt agcgggtgcc gctgctgaag gttctacggc
     2461 atcgggctgg acaaataatg gtgatgggac gtggactgcg cagattactc tcggctctac
     2521 ggcgggtgaa ttagaagtta tgccgaagct aaatggacag aatgcggcag caaatgcggc
     2581 aaaagtaacc gtggtggctg atgcgttatc ttcaaaccag tcgaaagtct ctgtcgcaga
     2641 agaccacgta aaagccggcg aaagcacaac cgtgacgctg gtggcgaaag atgcgcatgg
     2701 caacgctatc agtggtcttg cgttgtcggc aagtttgacg gggaccgcct ctgaaggggc
     2761 gaccgtttcc agttggaccg aaaaaggtga cggttcctat gttgctacgt tgactacagg
     2821 cggaaagacg ggcgagcttc gcgtcatgcc gctcttcaac ggccagccag cagccaccga
     2881 agccgcgcag ttgacggtca tcgccgggga gatgtcatca gcgaactcta cgcttgttgc
     2941 ggacaataag gctccgaccg tcaaaacgac gacggaactc accttcaccg tgaaggatgc
     3001 gtacgggaac ccggtcaccg ggctgaagcc agatgcacca gtgtttagcg gtgccgccag
     3061 cacggggagt gagcgtcctt cagcaggaaa ctggacagag aaaggtaatg gggtctacgt
     3121 ggcgacctta acgctgggat ctgccgcggg tcagttgtct gtgatgccgc gagtgaacgg
     3181 ccaaaatgcc gttgctcagc cactggtgct gaacgttgca ggtgacgcat ctaaggctga
     3241 gattcgtgat atgacagtga aggttaataa ccaactggct aatggacagt ctgctaacca
     3301 gataaccctg accgtcgtgg acagctatgg taacccgttg caggggcaag aagttacgct
     3361 gactctgccg aaaggtgtga ccagcaagac ggggaatacg gtaacaaccg atgcggcagg
     3421 taaagccgac attgagctta tgtcaacggt tgcgggagaa cacaatattt ccgcttcggt
     3481 gaatggtgct cagaagacgg tcacggtgaa attcaacgcg gatgccagca ccggtcaggc
     3541 aaacctgcag gtagacaccg ccgttcaaaa agtggcaaac ggcaaagatg cctttacgct
     3601 gacggcgacc gttgaggata aaaatggtaa ctctgttcca gggagcctgg tgacctttaa
     3661 tctgccccgg ggtgtcaagc cgcttacagg cgataatgtc tgggtgaaag ccaacgatga
     3721 ggggaaagca gagttgcagg tggtttcagt gactgccgga acgtatgaga tcacggcatc
     3781 ggcagggaat agccagcctt cgaatacgca gactataacg tttgtagccg ataaggctac
     3841 cgcaaccgtc tccggtattg aggtgattgg caactatgcg ctggcggacg gcaaagccaa
     3901 acaaacgtat aaagttacgg tgactgatgc caataacaac ctgttgaaag atagcgaagt
     3961 gacgctgact gccagcccgg cttcgttaaa cctggaaccg aatggcactg cgacaacgaa
     4021 tgagcaaggg caggctattt tcaccgctac cactactgtt gcggcgacat atacactcac
     4081 ggcgcaagtg agtcagaaca acggtcaggt atcaacgaaa accgccgaat ctaaattcgt
     4141 cgcggatgat aaaaatgcgg tactcaccgc atcatctgat gtgacttctc tggtggcgga
     4201 tgggaaatcg actgcgaagc tggaggtgac cctgatgtcg gcaaataacc ccgttggcgg
     4261 gaatatgtgg gtcgacattc agacgccgga aggggtgacg gagaaggatt atcagttcct
     4321 gccgtcgaaa aatgaccatt tcgtgagcgg aaaaatcacg cgtacattta gtaccagcaa
     4381 gcctggtgtc tatacgttca catttaacgc cctgacgtat ggcgggtacg aaatgaagcc
     4441 agtgacggtg accattaccg cggtggatgc cgatacggca aagggcgagg agacgatgaa
     4501 ataatattaa gaatatgaaa gtaacattct aataaacaat aatggcgttg acatcttcaa
     4561 cgccattatt tattgaattg cctgacagta gtctcgttat aagttcattt agatatagcg
     4621 acagctaaac cggacgaacg gggataaaaa tttcgcaaac aatgttattg ctatcaattt
     4681 cgacgaagtt tacattgtaa taaaaccgct caatatcact gccgttgact ctggctaacc
     4741 ccctggcggg taattccatc agatagaggt ttcgcgccca ttcagggtag tgctcccact
     4801 caccttcata gcggaaagag gcatacagtc ctcggttaat tgtccactct ttcggcatct
     4861 ctgaatcggt ttgtttgcat accgtaccgg caaaagtttc aaccgtgagc aaatcgaccg
     4921 attttgttga cggttcaaac gtggagacga aatagatatc cgtcttaaaa ttattcatac
     4981 atctggctaa atgatgacgt cgcagtttga cttcttcatc cggtgattta ttcgacactt
     5041 caatatcata tttaaatgaa ttgccaaaaa taggtgtctc aggaaagttg actaatctac
     5101 actccgtctt ttgctgttga cgtattaaaa aagaagggaa gatatttgcg agatcccaat
     5161 aatcacggtg gcggtataca cggggagagc agccgaataa ctttttaaat tcgcggctga
     5221 atgactgctg tgaatcaaaa tgcaaagaga gtgcaatatc aagcatagat ttcgcggtga
     5281 gccggacaag aatggcggca cgacaaagcc ttcgtttgcg aatgtattct cccaaaggca
     5341 catgcatgaa atttcggaac agaagctgga tgttgcgtct gctgtagcca gatttctgtg
     5401 cgatatcttc gattgaaata gggtgctcaa gattgcactc aatccactcc aggagctgct
     5461 gtagaatctt ctgcctgatc atcacattct ccgttaattc tttgtcatat cgcacatttt
     5521 cgttatgccc tgggccctaa gtgtatcaaa aacagcaagg cccaaggcaa aagcactgat
     5581 agcgtctgct tcattgcccc aaaaatagag aggtttcagt cctacattat taatgaattt
     5641 tttgcataag tgatatcaaa atccacatac taatttgagg ttacgtttta acgtagactc
     5701 attgttcatg cctaatggag ggactgacag tggaattttc cgtattaagt aacaatctga
     5761 aaatgccgat gatgggattt ggtgtttttc aggttaccga taaaaacgtg tgcaaacagt
     5821 cagtgttgaa cgctatccgc acggggtatc gactcatcga cactgcagcg gtatacggta
     5881 atgaagatgc cgtcggtgaa gccgttcgtg aagccatatc cgaaggttta tgtacccgag
     5941 aagagttatt tttaacgtct aaattgtggg tgcaggatat gttgaatcag gatacggcag
     6001 cagcaggtat tgaagcatca ttaaaaaaat cgggactaga atacttcgac ctctatttat
     6061 tgcatcaggc tatgcgcgat tatttcagtg cgtggcgtgc actcgaagat gcctatgaag
     6121 agggcaaatt aaaagcgatt ggggtttcca atttctatcc tcatgttctg gcgaactttt
     6181 gtgaaacggt aagagttaaa ccgatggtca accaggtcga gttgcatccc tattttgccc
     6241 aaccagaggc gctggcaacc atgaagtatt ataacgtgca gcctgaagca tgggctccgt
     6301 taggtggtgg acgacataaa ccctttgaaa ataatctgct tcagagtatt gcagatgccc
     6361 atcaaaaatc gatttctcaa gtcattctgc gttggaatat tcaacgggga gtggtcgtta
     6421 ttccgaaatc gacacatcaa cagcgtatcg aagaaaattt tgctatctgg gatttctcac
     6481 tgacagagaa agaaatggca caaattagtt cgcttgattt gggttatgtt ggggagtcgg
     6541 taaaacattt taatcctgaa tttgttcgtg gttgtcttgc tgtaaaaata catgattgat
     6601 attaatcatg tattttacct gagacgacaa gaatctttta acaggggagt gatattgatc
     6661 ttcactctgt catatctccg gtaatatggc gtcaggcttc atcatcagaa agggggcctg
     6721 acgcctgaaa aagtgaacaa cagacagtgt tcggattatc aatattcagt ttttaacgtt
     6781 gaactggatt cattactgcg ttgtttaaga atttcccgcg ccgaatctgc cattatcatg
     6841 acagcacctg ccaacatcag agtatctttc aataccaagc gaccagcacc ggataaataa
     6901 gggaaaccat gatgagcgtc acccaatgcg ggtacccatg cctccggggt ggtgattaaa
     6961 aatgagagtg ttaccaacgg tgtggtaaat gccatcagcc ctcccaataa acctaaccag
     7021 cgattgacag gattagccaa aaccagcaga gcaataatca cctccacgac gccaagaccg
     7081 ttggaaaaac cataggtatt attggccgat tgccatgccc ttgcttctgg tttgtattcg
     7141 ccttcgtgag tcagatactg tttatagtct tccgggtgtt cataaaagaa cgacattagt
     7201 ggactgtttg cgacgaatgg tgtaatgctg tctgcctcgt aagggacaaa ctttaataac
     7261 ccaatccaca taaaaacaat tgtaatactc agacgaatta atgtcaggcc aattttatct
     7321 ccccgactta acaggtgcag gtatttctcc attgtgagca tccttattta tacaatactg
     7381 ctgttgcggt tgaattatta tcaattcgca tcgatgtgac tttccaggat gaagcattat
     7441 attttctggc tatatcagca atggcatcct ccagaactgt cggtgatgct gatactgacg
     7501 agacgctgat atgtttcaga attattttat catctgcatt ggtggaaaat gccataacgc
     7561 cagataatag tgttgcaaat aaaacg
//
LOCUS       DAPGRN010000177         7263 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPGRN010000177 DAPGRN010000000
VERSION     DAPGRN010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.179"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            62..5332
                     /gene="traI"
                     /locus_tag="RHW33_004355"
     CDS             62..5332
                     /gene="traI"
                     /locus_tag="RHW33_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HDV1647793.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDVQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDVMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     KATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALNTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFADLGREIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGMEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDDRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTSDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5352..6098
                     /gene="traX"
                     /locus_tag="RHW33_004356"
     CDS             5352..6098
                     /gene="traX"
                     /locus_tag="RHW33_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDV1647794.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDVAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6153..6713
                     /locus_tag="RHW33_004357"
     CDS             6153..6713
                     /locus_tag="RHW33_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV1647795.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPELFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            6845..7057
                     /locus_tag="RHW33_004358"
     CDS             6845..7057
                     /locus_tag="RHW33_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647796.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAS
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1649 a         1825 c         2409 g         1380 t
ORIGIN      
        1 aggtgaacat taatgtgcac cgggagcgcg gggaggatgt tgagccggga gatgatttct
       61 gatgttgagt ttcagcgttg ttaaatcggc aggcagtgcc ggaaactatt acaccgacaa
      121 ggataattac tatgtgctgg gcagcatggg agaacgctgg gctggtcagg gggctgaaca
      181 gcttgggctg cagggcagcg tcgataagga tgtttttacc cgtcttctgg agggcaggct
      241 gccggacggg gcggatttaa gccgcatgca ggatggcagt aataagcatc gtcccggcta
      301 cgacctgacc ttctccgccc ccaaaagtgt ctccatgatg gccatgctcg ggggggataa
      361 acgtcttatt gatgcacata accaggccgt ggattttgct gtccgtcagg tggaggcgct
      421 ggcttccaca cgggtgatga cggacggaca gtcagaaacg gtgctgaccg gtaatctggt
      481 gatggcactg tttaaccatg acaccagtcg cgatcaggac ccacagttac acacgcatgt
      541 ggtggtggct aatgtcacgc agcataacgg cgagtggaag acactgagca gtgacaaagt
      601 ggggaaaacc ggattttccg agaacgtgct cgcgaaccgg attgcgttcg ggaaaatata
      661 tcagagtgag ttacgtcagc gcgttgaggc gctgggttat gaaaccgaag tggtcggtaa
      721 gcacggtatg tgggagatgc cgggcgtacc ggtggaggcc ttttccagtc gttcacaggc
      781 tatcagggag gccgtcggtg aagacgcctc gctgaaatcc cgggatgtgg cggcgctgga
      841 tacgcgaaaa tccaaacagc acgtcgatcc ggaaatcaga atggctgaat ggatgcagac
      901 gctgaaggaa accgggttcg acatccgggc atatcgtgac gcggcggatc agcgtgcgga
      961 gatccgcacg caggcgcccg ggcctgcttc acaggacggg ccggatgtgc agcaggcggt
     1021 gacacaggcc attgccggat taagtgaacg aaaagtgcag ttcacgtata cggacgtgct
     1081 ggccaggacg gtcggcatac tgccgccgga aaatggtgtg attgaacggg cacgcgccgg
     1141 tatcgatgag gccatcagcc gtgagcagct tatccccctc gatcgtgaga aggggctgtt
     1201 cacgtccggg attcatgtgc tcgatgagct gtcagtccgg gcactcagtc gtgacatcat
     1261 gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc ccccggacgg ctggttacag
     1321 cgatgccgtc agtgttctgg cacaggatcg cccgtcgctg gccattgtgt ccgggcaggg
     1381 cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc atgatggccc gggagcaggg
     1441 gcgggaagtg cagattatcg ctgctgaccg tcgctcgcag atgaacctga agcaggatga
     1501 acggctgtcc ggtgagctga taaccggacg tcgtcagctg ctggaaggca tggccttcac
     1561 gccgggcagt accgttatcg tcgaccaggg cgaaaaactc tccctgaaag agacgttaac
     1621 cctgctggat ggtgccgcac gtcataacgt acaggtcctg ataaccgaca gcgggcagcg
     1681 aaccggtaca ggcagtgcac tgatggccat gaaggatgcc ggggtgaaca catatcgctg
     1741 gcagggggga gagcagcgac cggcaaccat catcagtgaa ccggaccgta atgtccgcta
     1801 tgcccggctg gcaggggatt ttgcggccag cgtgaaagcc ggagaagaga gcgtggcaca
     1861 ggtcagcggg gtacgggaac aggccatact gacgcaggcc attcgcagtg agctgaaaac
     1921 acagggcgtg ctcggacatc cggaggtgac catgacggca ctgtcaccgg tctggctgga
     1981 cagccggagc cgttatctgc gggatatgta ccgtccgggg atggtgatgg agcagtggaa
     2041 cccggagaca cgcagtcatg accgctatgt gatagaccgg gtgacagcgc agagtcacag
     2101 cctgaccctg cgggatgtgc agggcgaaac gcaggtggtg cgtatttcct ccctggacag
     2161 cagctggtcg ctgttccggc cggaaaaaat gccggtggca gacggcgagc gactgagggt
     2221 gaccgggaaa attcccggac tccgagtctc cggcggtgac cgcctgcagg tggcatccgt
     2281 cagtgaagat gtgatgacgg ttgttgtgcc ggggcgggct gaaccggcct ccctgcctgt
     2341 gagcgattca ccgttcacgg cactgaagct ggagaatggc tgggtggaaa cgcccgggca
     2401 ttccgtcagc gacagtgcga aggtttttgc ctccgtcaca cagatggcaa tggacaaggc
     2461 caccctgaac ggtctggccc gcagcggtcg tgatgtccgg ctgtattcct cactggatga
     2521 aacccgtact gcggaaaaac ttgcccgcca tccgtccttt acggtggttt ctgagcagat
     2581 aaaggcgcgg gccggtgaga catcgctgga aacggctatc agtcatcaga agtccgcact
     2641 taacacgccg gcacagcagg ccattcatct tgcccttccg gtggtggaaa gtaaaaacct
     2701 ggccttcagc catgtggacc tgctgacaga ggcaaagtcg tttgctgcag aaggaaccag
     2761 ttttgctgac ctggggcggg aaatcgatgc gcagataaaa cggggcgatt tactgcatgt
     2821 ggatgtggca aaaggttatg gcacagacct gctggtttcc cgtgcgtcgt atgaggctga
     2881 aaaaagcatt cttcgccata ttctcgaagg taaggaggcg gtcacgccgc tgatggagag
     2941 agtgcctggc gaactcatgg agaaactgac atcaggacag cgcgccgcca cccgcatgat
     3001 actggaaacg tccgaccgtt tcacggtggt gcagggctat gccggtgtgg gtaagaccac
     3061 acagttccgg gcggtgatgt cagccgtgaa catgctgccg gagagtgagc gtccccgtgt
     3121 cgtcgggctg ggtcccacgc accgtgcggt cggggagatg cgcagcgccg gcgtggatgc
     3181 acagacactg gcgtcctttc tgcatgacac gcagctgcag cagcgcagcg gagaaacgcc
     3241 ggatttcagc aacacgctgt ttctgctcga tgagagctca atggtgggca ataccgacat
     3301 ggcacgggca tacgccctga ttgcggccgg tggtggtcgt gccgtggcca gcggtgacac
     3361 ggaccagctg caggccatcg cgcccggtca gcctttccgt ctccagcaga cgcgcagtgc
     3421 tgccgatgtg gctatcatga aagagattgt gcgtcagacg ccggaactgc gggaggcggt
     3481 atacagcctg attaaccggg atgtggaaag ggcattgtcc gggcttgaga gtgtgaaacc
     3541 gtctcaggtg ccacgtcagg agggcgcatg ggcaccggag cactctgtga cggagttcag
     3601 tcacagccag gaagcgaaac tggcagaagc gcagcagaag gcgatgctga aaggcgaggc
     3661 tttcccggat gtccccatga cactgtatga agccattgtc cgcgactata ccggcagaac
     3721 accggaagca cgggagcaga cgctgattgt cacacacctg aatgaggacc ggcgcgtact
     3781 gaacagcatg attcatgatg cacgggaaaa ggccggtgag ctgggcaaag agcaggtcat
     3841 ggtgcctgtc ctgaacacag cgaatatacg tgacggagaa ctgcgtcgtc tctccacctg
     3901 ggagactcat cgggacgcac ttgtcctggt ggataatgtg tatcaccgga ttgccggtat
     3961 cagtaaggat gacgggctga taaccctgga ggatgcggaa ggtaacacgc ggctgatttc
     4021 gccccgggag gcggtggctg aaggtgtcac actgtacacc ccggacacca tccgggtggg
     4081 gaccggtgac cggatgcgct tcacgaagag tgaccgggag cgcggttatg tggccaacag
     4141 cgtctggacg gtgacagcgg tttccggtga cagtgtcacg ctgtcggacg gacagcagac
     4201 ccgggtgatt cgccccggcc aggagcgggc agagcaacat attgacctgg cctatgccat
     4261 caccgcccac ggtgcgcagg gtgcgagtga aacctttgcc atcgcgcttg aaggcatgga
     4321 aggtaaccgg aaactgatgg ccggctttga gtcagcctac gtggccctgt cgcgtatgaa
     4381 gcagcatgtg caggtgtaca ctgatgaccg tcagggctgg acggatgcca tcaacaatgc
     4441 cgtacagaaa gggactgccc acgatgtgct ggagccgaaa gctgaccggg aggtcatgaa
     4501 tgcagagcgg ttgttcagta cggcacggga actgcgggac gtggcggcag ggcgtgccgt
     4561 tcttcgtcag gcggggcttg ccgggggaga cagtcctgca cggtttattg caccgggacg
     4621 taaatatcca cagccgtatg tggcactgcc ggcgtttgac cgtaacggca aatcagccgg
     4681 tatctggctg aacccgctga cctcggatga cggaaacggg ctgcggggat tcagtggtga
     4741 aggccgtgtg aaaggcagcg gggatgcgca gttcgtggcc ctgcagggca gccgtaacgg
     4801 agaaagcctg ctggctgata atatgcagga tggtgtccgg attgcccgtg ataatcctga
     4861 cagtggtgtg gtggtaagaa tcgccggtga aggccgtccg tggaatcccg gtgccatcac
     4921 cggtggccgc gtgtgggggg atatcccgga caacagcgtc cagccgggag ccggaaatgg
     4981 cgagccggtc acggcagagg tactggcaca gcggcaggct gaagaggcca tccgccgtga
     5041 aacggaacgc cgtgcggatg aaattgtccg taaaatggca gagaacaaac ctgacctgcc
     5101 ggatggcaaa acagagcagg ctgtcaggga gattgccggg caggagcgtg accgggctgc
     5161 cataactgaa cgggaagccg cgctgccgga gagtgtactg cgtgaaccac aacgggagcg
     5221 ggaagcggtc cgggaggttg cccgggaaaa tctgctgcag gagcgtctgc agcagatgga
     5281 gcgggatatg gtccgtgacc tgcagaaaga gaaaaccctg ggcggagact gatacaggaa
     5341 ggcaaacgct gatgacaacg gataacacga acacgacacg taacgattca ctggctgccc
     5401 ggaccgatac ctggttgcag tcgttgctgg tctggtcacc cggacagcgg gatatcatca
     5461 aaacggtggc actggtgctg atggtactgg accacatcaa tctgatattc cagctgaagc
     5521 aggaatggat gtttctggcc gggcgtggag cctttccgct gtttgccctg gtgtgggggc
     5581 tgaatctgtc ccgtcatgcg catatccggc aaccggccat taaccggctg tggggatggg
     5641 cggtgattgc ccagttcgca tattatctgg ccggctttcc ctggtatgag gggaatatcc
     5701 tgtttgcctt tgcagtggcg gcacaggtgc tgacatggtg tgaaacgcgc acctggtggc
     5761 gttccgcaga gaccatgttg cttctggcaa tgtggttgcc tttctccggc accagttacg
     5821 gcattgccgg gctgctgatg ctggcagtca gccacaggct gtaccgggcg gaagacagaa
     5881 tggaacgtct ggcgctggtc gcctgcctgc tggctgttat tccggcgctt aaccttgcca
     5941 ccagtgatgt ggcggctgta gccggccttg tgatgacagt gctgactgtt ggtctggtgt
     6001 cgtgtgcagg gaaatcctta ccccgtttct ggcccggaga ttttttcccg gtattctacg
     6061 cctgtcatct ggctgtgctg ggcgttctgg cactgtgacg ggtgtggtat ctttggccgc
     6121 aagaggatga ttcgtcagag gcagaacaca gcatgacaga gcagaagcga ccggtactga
     6181 cactgaagcg gaaaacggaa ggggagacgc ctgtccgcag ccggaaaacc atcatcaatg
     6241 tcaccacgcc accaaagtgg aaggtgaaaa agcagaagct ggctgagaaa gcagcccggg
     6301 aagcagagct ggcggccaaa aaagcgcagg ccagacaggc gctgtccatt tatctgaacc
     6361 tgcccacact ggatgaggcc gtgaacaccc tgaagccctg gtggccggaa ttattcgacg
     6421 gtgagacgcc ccggcttctg gcctgcggta tccgggatgt gttactggaa gacgtggcgc
     6481 agcggaatat cccgctctcg cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt
     6541 cagaaagcta tctgtgtgcc atgaaagccg gtgcctgccg gtatgacacg gaagggtatg
     6601 tgacggagca tatttctcag gaggaggaag cgtatgcggc agcgcgtctg gataaaatcc
     6661 gccgccagaa ccggataaag gcagaacttc aggccgtgct tgatgagaaa taaaaaaaag
     6721 cctccatcag agaggaggca gggaaataag gcttaaaagg aaataacttc cgtacccgaa
     6781 aggaagccaa ttcagtagat tatttaacac cataatactg acgggttaaa gaggagtgat
     6841 aaagatgaat ggattcagaa acagttcacg gaacggtcag gtctggcgct ggcagcgtgc
     6901 cggaagccgg gccgttattc tggaagtcag tggacgctgg atggaagcgg cagaagcatg
     6961 gcgacgggct gccagtgttg caccccggac agactggcag cagtttgccc gaaaaagggc
     7021 tgaacactgc caccggcgct gccggggaag ggtgtaacga aaaaagcagc cttcattact
     7081 ccaggtattc agaaggctga ataatgcatg agatttttct tttttatatc tggcctggca
     7141 gtatgaccag tcagcattca ttatcacagg attattgctg tattaagtaa tagcgtgctg
     7201 ttttgtgatt gatttcataa aacaagacgt cgtttaatat gcgcctgaat attcacgagg
     7261 gaa
//
LOCUS       DAPGRN010000178         7258 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPGRN010000178 DAPGRN010000000
VERSION     DAPGRN010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.180"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            194..382
                     /locus_tag="RHW33_004359"
     CDS             194..382
                     /locus_tag="RHW33_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HDV1647797.1"
                     /translation="MKDNILTFLTELKTPCRTTEIAIHFGLSAYQARYYLMNLEKEGK
                     IKRSPVRRGASTLWEMNS"
     gene            complement(477..1514)
                     /locus_tag="RHW33_004360"
                     /pseudo
     CDS             complement(477..1514)
                     /locus_tag="RHW33_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1708..3249
                     /gene="istA"
                     /locus_tag="RHW33_004361"
     CDS             1708..3249
                     /gene="istA"
                     /locus_tag="RHW33_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HDV1647798.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            3264..4010
                     /gene="istB"
                     /locus_tag="RHW33_004362"
     CDS             3264..4010
                     /gene="istB"
                     /locus_tag="RHW33_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HDV1647799.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            complement(4073..4576)
                     /locus_tag="RHW33_004363"
                     /pseudo
     CDS             complement(4073..4576)
                     /locus_tag="RHW33_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1705 domain-containing protein"
     gene            complement(4761..5507)
                     /locus_tag="RHW33_004364"
     CDS             complement(4761..5507)
                     /locus_tag="RHW33_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV1647800.1"
                     /translation="MKKVFAVSVVGAACVFAANAGAEDGKSGFYLTGKAGASVVSLSD
                     QRFLSGDEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NIDKGKGSWSDGYWRDDLKNEVSLNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKT
                     TGISSWDYGYGYSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDSS
                     VSYKDEWGDKYKSEVDVKSHDILLGVTYNF"
     gene            complement(5724..6092)
                     /locus_tag="RHW33_004365"
     CDS             complement(5724..6092)
                     /locus_tag="RHW33_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647801.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEIEKRKLSCRVYTEYRSTALAGSLWSPTVILGVASAVAIGVHNLSTWNPDYE
                     IGKNYIKRRLSVKYKKAEVL"
     gene            6324..6554
                     /locus_tag="RHW33_004366"
     CDS             6324..6554
                     /locus_tag="RHW33_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV1647802.1"
                     /translation="MQRHGSIETRTARDKAAERQMSHMDVTQCRPRIIHKAEYLDERK
                     LMLQWWVDFLDANRERAITPFDYAKINRGNGE"
     gene            6945..>7258
                     /locus_tag="RHW33_004367"
     CDS             6945..>7258
                     /locus_tag="RHW33_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647803.1"
                     /translation="MNFKKKIEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LK"
BASE COUNT         2086 a         1720 c         1631 g         1821 t
ORIGIN      
        1 ttaccggggg aaacaaaagc ggacagaaaa atgactgtgg aattttcttc ttccggcaga
       61 aaataatcgt tgtattatat acgtcagctg ccagccctga tggctttaca tggctgaaat
      121 taacaacgac aagagttcgt atggtttgat ataccatgca cgatattcag gtccacacgg
      181 gaattaacag aatatgaaag acaatatact gacgttccta acggagctga aaactccgtg
      241 ccggacaaca gaaattgcca tacattttgg gctgagtgcc tatcaggcca gatattacct
      301 gatgaatctc gaaaaggagg gaaagataaa acgctcgccg gtaaggagag gtgccagtac
      361 cttatgggaa atgaattcgt gacgggaaaa tatctgtgcc tgaaatataa aaatggtcaa
      421 cggttttccc gggttgtcag acgatgaacc atgccatttc gcaggtgtaa gaattattac
      481 agagaatacg tcgtccggca ctcaccgaac acctccatgc taatatgcag caggagttca
      541 tcaacacggc tggaaggcca ttccggcagt acgctcagga tatggcgcag atacgcttcc
      601 ggatcgatac cgttcagtcg gcaggtgccg atcagcccgt acagtagcgc tccccgctca
      661 ccaccatggt cagagccgaa gaacaggaag tttttacgac ccagactgac cgcccgcagg
      721 gcattttcag cgatgttgtt gtcgatttcc acccagccat cgttcgcata gtacgtcagt
      781 gccggccact ggttaagtgc gtacgcgaac gccttcgcca actctgagtg tcgcgacagg
      841 gtcttcatct tttcacgcaa ccagctttcc agggatttca acagcggttt cgtttttcgc
      901 tgacgttcag caagccgctg ctctgccggc attcccctta tatccgcctc tatggcgtac
      961 aactgaccga tctgctccag ggcttcttcc gtcagtgctg acgggatgcg gacgtgcaca
     1021 tcgtggatct ttcggcgggc atgagcccag caggcagctt ccgttatccc accattgcga
     1081 tacagctcgt tgaacccggc gtacgcatcc gcttgcagca caccgctgaa gcaagcaaga
     1141 tgagtctgcg gatggatgcc ttttctgtcc gggctgtaag cgaaccacac tgcaggtgcc
     1201 aacgctgacc cggcattgcg gtcatcacga acatacgccc acaaccgccc ggtcttcgtc
     1261 ttcttattac ccggcagcag tacctggacc ggggtatcat cggcatggag tttgccgtca
     1321 gtcatgacat cgccatgaag cgcctcttcc agcggagaca gcagccggca gcatgcatcc
     1381 acccagcccg acagcagtga acgcctcagc tccacacctt gccggccgta tatttctgac
     1441 tggcgataca gcggggtgtg ctctgcatac ttcgaggtca gcacgcgggc cagcagcccc
     1501 ggtccggcga tacctgcgta ttttcatgaa aggagatcac tcaataactt ccatcgagat
     1561 cgggtaataa catttgaaca gatcgctgaa taacatcgat ggagatcact tttgactcat
     1621 tttgttattc agtgatctcc atcaatgtta ttggaacttc acaggtgtgt tgatctgtat
     1681 cttttgccat tccggtaaag gatacctatg ccaacagttc caatttctat gagaaaactt
     1741 aaagaaattc ttaggcttaa atacggtgtt ggactcagcc atcgacaaat tggtcgtagt
     1801 cttgcaatct ccccttccgt tgtatccaga tatgctaatc gggcggctca acttggcata
     1861 aagcagtggc ccttacctac aggatgggat gatacaaaac taaaacatgc gttccttcag
     1921 acccaggtta agatgaagaa gcactctctg cctgactggg ctacagtaca ccgggaactg
     1981 cgtaataaat gcgtgacgct gcagctactc tgggaagaat actgtgagcg taatccaggc
     2041 ggtttttaca gctataacca ttactgccgg atgtaccgtg aatggctcaa aaccacttca
     2101 ccatcaatgc gtcaggtaca taaagctggc gaaaaacttt tcgttgatta ctgtggacct
     2161 accgttggcg ttaccgaccc tgagaccgga gaaataagaa ctgctcaggt catcgtagct
     2221 gttctcgggg catcaagtta cacatgggca gaggccacct ggtctcagca gcttgaagac
     2281 tgggtgatga gtcatgttcg ctgcttccag tggttgggtg gcgttcctga acttgttgtt
     2341 ccggacaatc tgaaaagcgc cacatccagg gcatgtaagt atgatcctga cgttaaccct
     2401 acctaccagc agatgcttga gcattataat gtcgcagttt tgcctgcgcg gccacgtaaa
     2461 ccgaaagata aagccaaagc tgaagttggc gttcaggttg ttgaacgctg gatcatggcc
     2521 cgaatcaggc atgagatctt ctacagcctt gcatcgctta atcagcgcat tcgggagttg
     2581 ctggaaagac tgaataacaa aataatgcag aagttgggtt attcacgtgc agaactcttc
     2641 atccagcttg ataaacccgc actgaagcct cttcctgaag ccagttacag ttacaccctg
     2701 gtgaagaaag tcagagttca tgccgattac cacgtggaaa tcgacaaaca ttactactcg
     2761 gttccatgtt cgctgttagg ccagcaactg gaagcatgga tctccggaga actggtaaga
     2821 ctcttcaatc aggggcagga ggttgctgtg cacccgcgca agcgtactta tggctacagt
     2881 acccgcaacg agcacatgcc tgaagctcat cgacagcatg ccacctggac gccagagcgt
     2941 cttctggaat gggcggggca cataggcagt gaaactcata gttatgtgct tcatatactg
     3001 aactctcgtc cacatccgga acaaagctat cgcttctgcc ttggactcct gaaccttcat
     3061 aaaaaataca gtaaagccag acttaatgca gcatgtgcaa gagctctgaa aacaaaggta
     3121 tggcgtctgt caggtattaa atcgatcctg gaaaaaggtc tggataaaca acctgttcag
     3181 gatccaaaac cagatctgtt atccacgatg gaacacgaaa acgtacgcgg cagtgagtat
     3241 taccactgat acgggatcca atgatgaatc atctttacga acaactgacc gcacttaaac
     3301 tcaccggctt ccgtgatgcg cttaaaaagc aacttgctca gccgggcaca taccaggagc
     3361 tgggcttcga agaacgcctg tcattactga cagcagaaga actaacctgc cgtgaaaaca
     3421 ggaaggcaga gcgtctgatc aaacatgcac ggttcagact taatgctgag ttatcaaagc
     3481 tggattatcg taacaataga gggctggaca gggccctcat ccgttcactc agtcagggaa
     3541 actggttaac cctgaaacaa aatattttac tgaccggggc caccggcagc ggtaaaacgt
     3601 tcctggcatg tgcacttggt cataatgcct gccgacaggg atacaaggtc tactattatc
     3661 gccttaaagc gctgatggaa cagtgctatc aggggcatgc tgatggaaga tacagcaaac
     3721 ttttgaccag gctgaataat agcgatctgc tgcttctgga tgactggggg ctggaacctc
     3781 tctcatcaga acagcgtagc gacctgctgg aaatagtgga tctgatgtac caacgaggct
     3841 caatcatcgt agtgagccag ttgccggtgg aaaactggta caaaatgatc ggagactcca
     3901 cacatgcgga tgccatccta gatcgactgg ttcatggcag tatcaagatc gaacttaaag
     3961 gagaatcaat gcggaaaata caatctccgt tgaccgaagg agatcagtga aggtaattta
     4021 aaaacggttc tgtgaaagtg acacgaaccg atctccatcg atgttactca ccgatctcct
     4081 tcacggtaat acgcatcccc acaacagttc ctttaaccat ccgcaacggc caactgttta
     4141 ctgagcataa tacgaatagc agcagtgcca tcccgttacg gtgaccacgg tgatgtgtcc
     4201 gccagaaaat caccatgaat accagaatcg cggcactgac cagaaaatga taaacaggaa
     4261 tcatcccggt aaactccgct gcctcatcag ttgtggtctg caacaacgcg acaataaaac
     4321 tgttgttgat tttaccgtac gtcataccgg caggcgcata cagtgcacaa cagaacagaa
     4381 ataacagcgc tgtaatggat gtgagggtat ttctgtgtgc aagaagcaga agaaagaaca
     4441 gcagcaacac attcccggtg gcattcttct cagtgtatcc gcatgcaatt gtggttatga
     4501 cagaaacaac aaaaaagaat aaaaacaata taatccgggg agtgttgccc ggacaaaaca
     4561 gttttcttat attcatcgga gtatatcgac aacattatta tgaagagaac aggataataa
     4621 aaatcagaag ttatctgtga aacagataac agacaaccct gcagtataat attactgcag
     4681 ggtgttcctt tttaattaca gaaatacgta attatcctaa atgcagaaat atgcgcaatt
     4741 atcgttcaga agcagtgtcg tcagaagtta taagtcacac caagcaggat gtcatgactt
     4801 ttaacatcaa cctctgattt atatttatcg ccccactcgt ccttgtaact cacactgctg
     4861 tcacccgcat caagatagcg atagctgagg tccagagcga tatccggggt gacgtcatag
     4921 cggacacccg cgccaaggct ccatgcgaag ttgtcagcag agcctgaacg tgacaacgat
     4981 tcacgaccac tgtatccgta cccgtaatcc cagctgctga taccggttgt tttctgatga
     5041 atcctggcgt aaccaatccc tgctgatacc catggtgtga atgcactgtc attccggaag
     5101 tcatagtatg cattcagcat cagagtgttg agtgatacct cattcttaag gtcatcacgc
     5161 cagtaaccat ctgaccagct gcctttacct ttatctatgt tatacttcga atcagctttt
     5221 ccacgtgcgt aaaactccag ttctgtacga accggaatac tgaactgcgg ataaaaatca
     5281 taaccggctg cgataccgcc actgaatacc gtgtcatggt catccccacc tttatacttt
     5341 gacgtttctt cttcatctcc tgacaggaaa cgctggtctg aaagtgacac cacagaggca
     5401 ccggctttac cggtcagata aaaaccgctt ttaccgtcct cagcacctgc atttgctgca
     5461 aatacacagg ctgcgcccac cactgaaaca gcaaaaacct ttttcatttc aattatctcc
     5521 gttattgcat catcacttta atatatattt taatatttaa aaaccggact aaaggcagcc
     5581 tcccccctcc acactacgga agccagactt ttactctaca caaaataaat atatttcctc
     5641 agtgaggacg tgaacgccag tctgaaatta tgtgtataca acctcaccag aatacgaaca
     5701 ggcaggggca ctggagtaac cgatcacaat acttctgcct ttttatattt taccgataat
     5761 ctccgtttaa tatagttctt accgatttca taatcggggt tccatgtaga taaattatga
     5821 acacctattg ctactgcact cgccactccc agaattacgg ttggtgacca taaagaacca
     5881 gctaaagctg tgctcctgta ctcagtatat acacgacaac ttaatttcct tttctctatt
     5941 tcatgctcca ctctttttac cgtttcacta acatcggaaa cattttctat cacaaaactc
     6001 ttacgttcat agataagctt gcttaatgta tttatatcta aaaatttcgc atcaaaagaa
     6061 tcaatctgta ctgttttttc ttgtgctgac atcttctttt cctggctggt atggcaagtg
     6121 acggagacaa gagaaacttt ttaagctcag ttatctccgc catcattttc cacgaatgac
     6181 aagtaatttt gcctatttta aaaccatgca aaaggcaggg taaaaagaga aaattcgatc
     6241 gaatcgatcg acaaaatcga tcatgcatga tgaagatttc ttatcgaaga catgaaaatg
     6301 gtaaggaatg gtttaagagg taattgcaac ggcatggaag tattgagacc agaacagcac
     6361 gagataaagc ggctgaacgt cagatgagcc acatggacgt aactcagtgc aggccgcgta
     6421 taatccataa agcggaatat ctggatgagc gtaagctgat gttgcagtgg tgggtcgatt
     6481 ttctggatgc caatcgggaa agagcgatta cgccgtttga ctatgcgaag atcaatcggg
     6541 gtaatggcga gtgagttgtc taaaagtgca cattcgcctc taaattaaaa gctgctgact
     6601 tattcaaacg aggaattggt tagcagtgcc agatagccta gttttcacta aggtaagcct
     6661 ttttattgaa cctaatcggc agtaatgctc cacctaagcc tagatttaaa atcatgatta
     6721 cttataaaag gggagtagcc ccttgattga gcgctacatc gtaacaaagg tacagcaatt
     6781 cggctacatc aggagtgata gccaattagt taatctaata attaattgta tctaaataat
     6841 taaatttctt acgtaaaatt gaccaatgta ctggtggtgt aagccgcaat accttaatgt
     6901 aatcggtata aaggggcttg atttctttta aagagagtat ccgtatgaat tttaagaaaa
     6961 aaatcgaaga gcattttaag cagttcgaag catcccctgt gttatttgtt ggctccggtg
     7021 tatctcgtcg ttatcttggt gtgccatgtt ggcaagacct tttgaagcat tttgctgagg
     7081 cgattgggga aaatcatata aaattaaaaa caaaatctaa tggtgactta cccgaatatg
     7141 cacaattatt ggtgtcggca tacgctgaga aatggtggga tactgaagaa ggacaattag
     7201 cattaagtga aaaagaacag gagaaaactt tcattaacga acagtctcct ttgaaatt
//
LOCUS       DAPGRN010000179         7118 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPGRN010000179 DAPGRN010000000
VERSION     DAPGRN010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.181"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..163
                     /locus_tag="RHW33_004368"
     CDS             <1..163
                     /locus_tag="RHW33_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647804.1"
                     /translation="TITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLF
                     LMRDIIISS"
     gene            124..243
                     /locus_tag="RHW33_004369"
     CDS             124..243
                     /locus_tag="RHW33_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV1647805.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            278..622
                     /locus_tag="RHW33_004370"
                     /pseudo
     CDS             278..622
                     /locus_tag="RHW33_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(994..1260)
                     /gene="minE"
                     /locus_tag="RHW33_004371"
     CDS             complement(994..1260)
                     /gene="minE"
                     /locus_tag="RHW33_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV1647806.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1264..2076)
                     /gene="minD"
                     /locus_tag="RHW33_004372"
     CDS             complement(1264..2076)
                     /gene="minD"
                     /locus_tag="RHW33_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV1647807.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2100..2795)
                     /gene="minC"
                     /locus_tag="RHW33_004373"
     CDS             complement(2100..2795)
                     /gene="minC"
                     /locus_tag="RHW33_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV1647808.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPASQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3314..3682
                     /locus_tag="RHW33_004374"
     CDS             3314..3682
                     /locus_tag="RHW33_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HDV1647809.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMVSGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3802..4203)
                     /locus_tag="RHW33_004375"
     CDS             complement(3802..4203)
                     /locus_tag="RHW33_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV1647810.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4445..4738
                     /locus_tag="RHW33_004376"
     CDS             4445..4738
                     /locus_tag="RHW33_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV1647811.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4810..5469
                     /locus_tag="RHW33_004377"
     CDS             4810..5469
                     /locus_tag="RHW33_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV1647812.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGLVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5561..6007
                     /locus_tag="RHW33_004378"
     CDS             5561..6007
                     /locus_tag="RHW33_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV1647813.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYDRRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6214..7116)
                     /gene="hlyE"
                     /locus_tag="RHW33_004379"
     CDS             complement(6214..7116)
                     /gene="hlyE"
                     /locus_tag="RHW33_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131199601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDV1647814.1"
                     /translation="MVANKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         1828 a         1570 c         1685 g         2035 t
ORIGIN      
        1 aacgattaca ttagtcattt tattttgccg acggcctcat tgtcgaaaga taagcgtacg
       61 acagtattat ctgaaaagag tgatttttta tccaactaca cttcagcgca ctgcgtttaa
      121 aaaatgcctc tttcttatgc gggatatcat catttcatca tgatgtcttt ggtgagcggt
      181 gaacacaata cacctgcgct gtctcttcag gatgaatccc ctggtctggt gcctgcgggc
      241 tgatgttgca gccgagctaa ggtcacttag acgctactat catttatcca atggcatgga
      301 atcgaaatca gtcgataccc gcagtatata tcgtgaactg ggcgcaacgc tgagttacaa
      361 catgcgtctg gggaacggta tggaagttga gccgtggctg aaggcggctg tgcgcaaaga
      421 atttgtcgat gataatcggg tgaaggtgaa taatgacggt aattttgtca atgatttgtc
      481 gggcagacgt ggaatatacc aggcaggtat taaagcctca ttcagcagta cgttaagcgg
      541 gtatctcggg gtggggtata gccatggtgc tggtgtggaa tccccgtgga atgcggtagc
      601 tggtgtgaac tggtcgttct gaccatcaac gattaaactg cacttcggca cagttttcgt
      661 ttacagaatg ttaaaagaga aaattctggg gtaaaaaaag cccgccagtt acggcgggaa
      721 acctcatcct atgggagaac aatgaataat gaaattgcgg ggttatcatc tcccagtata
      781 tccacactaa caataaggtt atttactcaa ccaggcataa atattttgtt ttgtgcgtgg
      841 gaacagcctt aaggtgtaaa gggggaggtg gaaatagcaa tgaggagtat cagcaagaat
      901 actcgccgct ttaccacaac gtggatgaga gggatgaaaa actcaaggca gagataactc
      961 tgccttgaag ataaatgcgc ttttacagcg ggattatttc agctcttctg cttccggtaa
     1021 ggtcacgttc agctcaagaa tagaaatatc gccatctttt tgctcaagct gtacggttac
     1081 catctcagga tcaatttgta cgtatttaca aatgacctca agaatatctt tacgcaactg
     1141 cggcagataa tgcggttctg catcgctgcg acggcgttca gcaacaataa tctgcagccg
     1201 ttcttttgca atgttggctg tgtttttctt ccgcgagaga aagaaatcga gtaatgccat
     1261 aacttatcct ccgaacaagc gtttgaggaa gcctttcttc tcttcttcaa tgaagcggaa
     1321 aggacgttct tctcccaaca gacgttctac ggtatctgcg taggctttac ccgcatcggc
     1381 gttaatgtcg agaatgaccg gctcaccctg gttagaggcg cgcaatactg attgatcctc
     1441 tgggatcacg ccgacgagtt tgatgcgcag gatctccagc acatcttcca tgctcagcat
     1501 gtcacctctg cttacgcggc ctggattata gcgcgttaac agcaggtgct ctttaatagg
     1561 ctcttcgcca ttttctgcgc ggcgtgattt tgacgccaga atgcctaaaa tacggtcaga
     1621 gtcgcgtact gaagagactt ccgggttggt ggtaataatg gcttcgtctg caaaatagag
     1681 tgccattaac gcaccggttt caatccctgc cggggagtca caaacgataa attcaaaatc
     1741 catcgctttc agatcatcaa gaactttagc gaccccttca cgggtgaggg catctttatc
     1801 gcgtgtttgc gatgccggca gaatatagag attttcagta cgcttatctt taattaacgc
     1861 ctgatttagc gttgcatcgc cctgaatgac gttgacgaaa tcgtaaacga cccggcgttc
     1921 gcaacccata atcaggtcga gattacgcag gccgatatca aaatctatca cgacagtttt
     1981 ctttcccttc tgggccaaac cagtggcgat ggccgcgctg gaggttgtct taccaacacc
     2041 ccctttgccc gaagtaacaa caataatgcg tgccatagaa attccttgtt aaaaagggat
     2101 caatttaacg gttgaacggt caaagcgttt tcgactaact gcagtcgcgc cgctttgcca
     2161 taaaattctg ctgggatttg atcactcagc cagtattcac ctgcgatgga caccaattcc
     2221 gccatcaggt tcgtacaaaa tatttgcgtt tcccggtcac cacttgcccc tgcaagcgca
     2281 cgaccgcgca tcatgccata gacatgaatg ttaccatcgg caatcaattc ggccccagcg
     2341 ctaacgtggc ttgtaacaat cagatcacat tgtggagcat aaatacgctg accggaacgc
     2401 accggggtat ctattaaacg cgtttttgtg accggcgttg tattttgcgc tggagcctgc
     2461 gatgcgggag ctggacgcgg cgctttttcc tttccttccg tcaggatagg cagccccatc
     2521 ttttcaattt cggctttaag ttgcgcatct ttgcagccac ttacgccaat aacccgcaaa
     2581 ccggttgccg aaaccgcctt atgcatcgct gaccagttta ccgggtcttc cagtgcactg
     2641 acgttgagta caacgggggc atgttttaaa aatgcggggg cctgagcgat tttgtcttcc
     2701 agcgcctgat ggataacctt aggttctgcc tcatgcagat gaaccacaga taaagtgaag
     2761 ctactgcctt taagctcgat tggcgtgttt gacatcctgg ccttactcaa ttagctatta
     2821 atcatcgcca gcgcgcgatg atgttccgaa gactataagg catgttatag tctggattat
     2881 attgaggcaa gtcaccctcc catttattca gagtaaaagt ctattctgtg ataaatggcg
     2941 ctgatccata gcttaaaaaa tacccttgtc aatcaaccca ttgccgtcgt acttttgatt
     3001 cttcttattt acgcttcctt tttccgcacc ctaatcgtga cagcatcttc agacctgttt
     3061 aaatatccag tattgaggca agtgtatttt ttcaccacgc caggctttct taactttcca
     3121 tttttgcagc atcattaaag aacagatacc cgttatctta atatccgcca cagaattgct
     3181 ctatcataga aaccgcagaa gacgtctgat acttctgtct ggtcatcgta actcgtccag
     3241 gtcagcggtt ttgattacgg attgatgatg aaatgagcat aagtgttgta cggttggatt
     3301 atcaggatgt gctatgaata tgatgcgtat tttttatatc ggattgtcag gtgtggggat
     3361 gatgttctca tcaatggtgt ctggaaatga cgctggcgga cttcaatctc cggcgtgcgg
     3421 cgttgtttgc gacccgtata tttgtgtgaa ctcagatggc atttccccag agttaacaag
     3481 gaaatatctc ggcgaaaaag ccgctgaaaa cttacaatca ttacaaggct acgatcccag
     3541 cgaatttaca ttcgctaacg gtgtattttg cgatgttaaa gaaaaattat gtcgtgatga
     3601 tcgttatttt ggtgtggatg gtaagcggag tggaaaaatc aatcaaacca ccacaaagat
     3661 gttatttatg tgtcgtgagt gatgccttgt ttacattatg ttctattcgg cattgtctgc
     3721 tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtt tatgacgggg
     3781 agagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt ttttagtttt
     3841 gtttttacgg gcgtcgtttc gggtttgcag cactcgcagc tcatatttgc cagaagcagg
     3901 gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat caacagaatc
     3961 atcgattcct ggcccgaaga ggtaagtatc agcgccttca ttagagatac tcacatgcac
     4021 tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc
     4081 ggagtactga gcgctgctat ggccttttct gaactcaaca ttgacattct tacccgcagc
     4141 aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc tcttgatttt
     4201 cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa caatccctta
     4261 cgggggaatt ccagtggctt tcctgcgttc ggtaaaatcc cgtcgcggca tttttgccgg
     4321 ggggattact tattaagggc gtatggactg aacaaaccgt agaaatctac agctggctgg
     4381 cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct taaagagtaa
     4441 aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt tatatgtcga
     4501 aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg gtcagcctca
     4561 gttagcgatg attctgccgc tggatgggcg gaagaaactg gtcaatgccg atattgaaaa
     4621 ggttaaacag gcattaaccg agcaaggtta ctatttgcag ttaccgccac cacccgaaga
     4681 tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta acaaataacc
     4741 gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg ataaggtgag
     4801 gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta tccggtgagt
     4861 aaagtagttt gtgttggcag taactatgcc aaacatatta aagagatggg cagcgcagtg
     4921 cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct gcggcagcca
     4981 ttggcgatcc catccgattt cggtttagtt catcatgaag tcgaactggc ggtgttgatt
     5041 ggcgcgacgc tgcgtcaggc tacggaagag catgtccgca aagccattgc cggttatggc
     5101 gtggcgctcg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg
     5161 tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat tcccgcggcg
     5221 gaatttaccg gcgacccgca aaatacaaca ctgggcctga gcgtaaacgg cgaacaacgc
     5281 cagcaaggta cgactgcgga catgatccat aaaatcgttc cgctgatcgc ttatatgagc
     5341 aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc
     5401 ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt gacaactcgc
     5461 gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac
     5521 tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg tacctttctg
     5581 gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg
     5641 cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct acttcactaa
     5701 cgtcgcctgt cgccagctca atattaaaac ctgtcaatgt cggaactacg accgtcgttt
     5761 cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct
     5821 gccaatgacc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg cgtggcatcc
     5881 gctgcttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc
     5941 agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac ctgactgggc
     6001 gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct ggatgctcgc
     6061 catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc gataccttat
     6121 tatcaacagg taactctcaa tctttaaaaa acgtctgtat ttttaaatgt gataaacttt
     6181 ataccttgag tacatggaga gaataattgc tgatcagact tcaggtacct caaagagtgt
     6241 cttcttaccg tgtcttttct gatactcatt acaggtgtta atcatttttt tggcggcttc
     6301 ttttagcaaa gaaagcatta aatcatcata atcaacatag aatctggttg tttcagtttc
     6361 tgtttttatc tccccgatgg cggctatttc ggtggttaat ttcaatttgg cggcatcgat
     6421 atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct gcacagattt
     6481 taacttgttc ttcaattctg gaatcagttt cccttcaact acgcccgcag caatagaata
     6541 ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg catacgcttc
     6601 cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa aatcattggt
     6661 taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt gtgagcttac
     6721 cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta ccttaatgag
     6781 aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca aaatatacgc
     6841 tgcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg cttcaaaata
     6901 cttatcctgg ctatccataa gtaaggtttt aatatcgcca actaaaacgg aggctgcctg
     6961 tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg tctgccaggg
     7021 gatgacctga tcgagatatt tattataaag atccaatgct ccatctgcgg tttcgattgc
     7081 gtttttaact acctctaccg ttttatttgc aacgattt
//
LOCUS       DAPGRN010000180         7069 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPGRN010000180 DAPGRN010000000
VERSION     DAPGRN010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.182"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            188..748
                     /locus_tag="RHW33_004380"
     CDS             188..748
                     /locus_tag="RHW33_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1647815.1"
                     /translation="MFSTFKKAALLAAIALPFSTMAAPTVTFQGEVTDQTCSVNINGQ
                     TNSVVLLPTVAMADFGATLADGQSAGQTPFTVSVSNCQAPTGADQAINTTFLGYDVDA
                     NTGVMGNRDTSSDAAKGFGIQLMDSSTSGNPVTLAGATNVPGLTLKVGDTEASYDFGA
                     RYFVIDSAAATAGKITAVAEYTLSYL"
     gene            834..1535
                     /locus_tag="RHW33_004381"
     CDS             834..1535
                     /locus_tag="RHW33_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309306.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1647816.1"
                     /translation="MNIFAYLLVLVFSMSMSSSAFASVVMTGTRIIFPGDAKEKTIQL
                     RNTSDQPYIINIHVEDERGSDKNVPFMPTPQTFRMEAAAGQALRLLYTGNNLPQDRES
                     VFWFSFSQLPYLNKNDKSQNQLILALTNRVKIFYRPSSIVGKSSDAPKNLTYQVKQNR
                     IEVTNPTGYYVTIRAAELLNNGKKIPLANSVMIAPQSTTEWTLPSGISVAPGAQIHLV
                     TVNDYGVNVTSEHAL"
     gene            1588..4110
                     /locus_tag="RHW33_004382"
     CDS             1588..4110
                     /locus_tag="RHW33_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1647817.1"
                     /translation="MNKSVVSISAAMLVLLCQPVMGSEISPATPSDEDNYTFDPQLFR
                     GSRFSQSSLAKLTTRESVAPGNYKMDIYTNNKLSGSWNVTFKEAADGRVLPCLTPEVA
                     DAIGLKTGEDKGEKDPVCTFAKELAPGITSQTQLSQLRLDLSVPQSQLISRPRGYVPP
                     SELDTGASLAFMNYIANYYNVAYSGQNAHSQRSLWASFNGGINLGAWQYRQLSNMTWD
                     NDKGNQWNNIRSYLQRPLPAINSQLMMGQLITSGRFFSGLSYHGVSLATDERMLPDSM
                     RGYAPTIRGVAATNARVSVMQNGHEIYQTTVAPGPFEINDLYPTSYSGDLDVTVTEAN
                     GAVSRFSVPFSAVPESMRPGTSRYNVEVGKTQDSGDDSMFGDFTWQHGLTNTLTFNSG
                     SRIADGYQALMLGGVYGSTLGAFGANLTWSHARVPESEAQSGWMSQLTWSKTFQPTST
                     TVSLAGYRYSTSGYRDLADVLGERQAASNKQSWDSSQWRQQSRFDLTLSQSLANYGNL
                     FVSGSTQNYRGGKSRDTQLQLGYSNSFSHGISMNLSVGRQRMGGYKDNSDDMQTVTSL
                     SFSFPLGGNGPRVPSLSNSWTHSTDGSSQLQSSLTGMLDEAQTTNYSLNVMRDQQYKQ
                     TTLSGNMQKRFSQTTVGLNASKGQDYWQASGNVQGAMAVHGGGVTFGPYLGETFALVE
                     AKGAEGAKVYNSSQLEINDSGYALVPAVTPYRYNRISLDPQGMDGDAELVDSEKQVAP
                     VAGAAVKVVFRTRPGKALLIKSRMADGSELPMGADVLDENNTVVGIAGQGGQIYLRTE
                     QTKGHLSVRWGEGANDSCQLPFDISGKDSNSPIIRLNETCQS"
     gene            4126..5460
                     /locus_tag="RHW33_004383"
     CDS             4126..5460
                     /locus_tag="RHW33_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1647818.1"
                     /translation="MSKSTFLHILISSIILVALIQSSAWATCSNTRVGQTEDGRSALI
                     EFGKINLTDTYFAPVGSLLATTVVPSTNYTSGGASGSSVLWECDATDLPNIYFLVATN
                     GDDRVGGFHNAGGPDGLSDVYATWFAFVGLKQTMAGVTIGRYWKKVPITSYATQGTKI
                     QIRLQDIPPLHAELYRISTLPDTAGATSYCGNTNADGDGVGYAKPSGTPYNCTQPNAY
                     IQLSGTSGIPFAHDEVGEDSADHYDFWGGDNGFGYGMRSVNRLYNNATCVARSATPLV
                     LLPTIAEAQLNAGMESTANFNVRVECSNSVQSGISDTQTALGIQVSEGAYTAAQKLGI
                     INSNGGVSALVSDNYDAAEMAKGVGIYISNSAQPDTEMTLVGQPGIAKLTPGGNTAGW
                     YPVFEGATLEGATHPGYSGYSYSFIARLKKLPNQTVSAGKVRATAYILVKMQ"
     gene            5457..6146
                     /locus_tag="RHW33_004384"
     CDS             5457..6146
                     /locus_tag="RHW33_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1647819.1"
                     /translation="MKSVLLLLTVLFVQSVEAGIMPASSRIIYRDGDREKTLMLVNTN
                     DYPVLVQSWVDNGEGSPEAASPPFVVLPPVFRLAARRTQGLRIIYNHDPLPQDRESAF
                     WLNLYEMPPETPHQDPTHLTLAMNTQLKIFYRPKSVTMTPQEAMRKISWHLVNNGSSS
                     YIELNNPTPLNISFTSISIGATKVEQESDMMIKPFSQRRYWLASKNDSGIVTATYLDD
                     DGAQIKQQLAQ"
     gene            complement(6290..7060)
                     /locus_tag="RHW33_004385"
     CDS             complement(6290..7060)
                     /locus_tag="RHW33_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1647820.1"
                     /translation="MEDFSLDFFSLHNKVAIVTGAAGELGRGLCSALAKAGANLLLVD
                     IKEPDNKYLKHLTHEGVEVEFMTIDITKPDATSTIINRCLERFGQLDILVNNAGVCNI
                     NRPIDFNRNDWDPMINLNLNAAFDMSQAALNIFVPQRKGKIINMCSVLSFHGGRWSPG
                     YAATKHALAGLTKAYADDFAEYNIQINGIAPGYYVSEMTAIIYNNPKIKELIKGRIPA
                     QRWGRAQDLMGAMVFLASAASDYVNGQLLVIDGGYSIR"
BASE COUNT         1899 a         1745 c         1650 g         1775 t
ORIGIN      
        1 caagtacatt agtcactata aaatatcagt tttcaacaag tttgaaattg cttgtgatag
       61 tgatggcttt tacattttca aggcacttat acagttaagg agatttaccg gttttaaaaa
      121 cgtcagattg tatcaattgc attaattaac tcgttttata ttaactctaa atttcaagga
      181 tattaatatg ttttctactt tcaaaaaagc agctctgctg gcagctatcg cattaccttt
      241 ttctaccatg gctgcaccga cagtcacttt tcagggtgaa gtaaccgatc agacctgttc
      301 cgttaatatc aacggtcaaa ccaattcagt agtattgctg ccgaccgtag ccatggctga
      361 cttcggtgca actttagctg atggccagag cgcaggccag acgccgttta cggtttctgt
      421 gtctaactgc caggctccga ctggtgcaga tcaggcaatc aacaccactt tcctgggcta
      481 cgacgttgac gctaacacag gtgttatggg aaaccgtgat accagcagcg acgcggcgaa
      541 aggctttggc attcagttaa tggattccag cacttctggt aacccagtaa ctctggctgg
      601 cgcgactaac gtaccgggtt tgaccctgaa agttggcgat actgaagcca gctacgactt
      661 cggtgcgcgt tacttcgtta tcgatagcgc agctgccact gccggtaaaa ttaccgctgt
      721 cgcagaatac accctgagct acctctaatt aatctactgg ctggcagtaa atgccagcca
      781 gtatttcttc gcacaattaa tagtgatttt tcatggacat ggaaaagata agaatgaaca
      841 tttttgcata tttactggta cttgtatttt ccatgagcat gagcagcagc gcgtttgcca
      901 gcgtggtaat gaccggaacc cgtattattt tccctggtga cgcaaaggaa aaaaccatcc
      961 agttgcgaaa taccagcgat cagccctata tcattaatat ccatgttgag gatgaacgtg
     1021 gttctgacaa gaatgtaccg tttatgccaa ccccgcagac atttcgcatg gaagctgccg
     1081 caggtcaggc gttacgcctg ctctacactg gtaataattt accgcaggat cgcgagtctg
     1141 ttttctggtt tagtttcagt caactacctt atctgaataa gaatgataaa agtcagaacc
     1201 agctcatcct ggccctgact aatcgagtca aaattttcta tcgtcccagc tcgattgtcg
     1261 gtaaatccag tgacgcaccc aaaaacctga cttaccaggt aaaacagaac cgcattgaag
     1321 tgacgaatcc cacgggctat tacgtcacaa ttcgcgccgc tgaactgctt aataatggta
     1381 aaaaaatacc cctcgcgaat tcggtaatga ttgctcctca aagcacaact gaatggacac
     1441 taccctctgg aatcagtgtc gctcccggtg cgcagatcca tttagtgacc gtcaacgact
     1501 atggcgtaaa tgttacgtct gagcatgcct tataacctct ttctcctttt ttgagcggta
     1561 tcctttttca cgcacgagta cgtcgagatg aataaatccg ttgtgtcaat ttctgcggca
     1621 atgttggttt tactttgcca accggtcatg gggagcgaaa tctcacccgc aacaccgtca
     1681 gatgaagaca actacacctt tgacccgcaa ctcttccgcg gcagcagatt tagtcagtcg
     1741 tcattagcaa aactgacaac acgtgagtct gttgcaccgg gcaattataa aatggatatc
     1801 tacaccaaca ataagttgtc aggcagttgg aatgtcacgt ttaaagaagc cgctgatggt
     1861 cgcgttctgc cctgcctgac gcctgaagtc gcggacgcga taggcctcaa aacaggggaa
     1921 gataaggggg aaaaagatcc tgtctgtacg tttgctaagg aactcgctcc cggcatcacc
     1981 agccagacac agttgtcaca attgcgcctg gacttatcgg tgccacagag tcaattgatt
     2041 agtcgccctc gcggctatgt tccccccagc gagctggata ccggagcatc gctggcgttc
     2101 atgaattata ttgccaacta ttacaacgtt gcctattcag ggcagaatgc tcatagccag
     2161 cgttcgctat gggcatcatt taatggcggc atcaaccttg gtgcctggca atatcgtcag
     2221 ttatccaaca tgacctggga taacgacaaa gggaatcagt ggaacaatat tcgtagctat
     2281 ttgcaacgcc cgctgcccgc cataaatagt caattaatga tggggcaact tatcaccagc
     2341 ggaagatttt tctctggact cagttatcac ggcgttagtc tcgcgaccga tgaacgtatg
     2401 ctgccggact ccatgcgcgg ctatgcgccg actattcgcg gggtggcagc aacaaacgcc
     2461 agagtctcgg taatgcaaaa cggtcatgaa atttatcaga ctaccgtggc tcctgggcct
     2521 ttcgaaataa acgacctata ccccaccagc tacagcggcg atctggatgt caccgttacg
     2581 gaagctaatg gcgcagtcag tcgtttcagt gtcccctttt cagccgtacc agaatcgatg
     2641 cgtccaggaa cttcccgtta taacgttgaa gtaggtaaaa cgcaggatag cggtgatgac
     2701 tcgatgtttg gtgacttcac ctggcagcac gggctgacta atacgctaac atttaacagt
     2761 ggctcgcgta tcgctgatgg ctaccaggcg ctgatgcttg gcggagtcta tggcagtacg
     2821 ctgggggcat ttggggcaaa cctcacttgg tcccatgcgc gtgttcccga aagcgaagcg
     2881 cagagtggtt ggatgtcgca attaacctgg agtaaaactt tccagcccac gtctaccacc
     2941 gtctccctgg caggttatcg atactctacc agcggctatc gtgatctggc tgatgtgttg
     3001 ggagagcgtc aagctgccag taataaacag tcatgggact ccagccagtg gcgtcaacag
     3061 tcgcgcttcg accttacgtt aagtcagagc cttgcgaatt acggcaacct gtttgtgtca
     3121 ggttcaacac agaactaccg tggcggcaag agccgtgata cacagcttca gttaggttac
     3181 agcaatagct ttagccacgg catcagtatg aacctttccg tcggacgcca aagaatgggc
     3241 ggctataaag acaattctga tgatatgcag acggtaacgt ccctttcatt ctcattccca
     3301 cttggcggca atggacctcg tgtaccaagt cttagcaaca gctggaccca ttcaactgac
     3361 ggtagctcgc aattacaaag ctcgctaacc ggaatgcttg atgaagcaca gaccaccaac
     3421 tacagcctga acgtcatgcg cgatcaacaa tataagcaga cgacgcttag cggaaacatg
     3481 caaaaacgtt tttcacaaac taccgtcgga ttgaacgcat cgaagggcca ggattactgg
     3541 caggcgtccg gaaacgtaca aggcgcgatg gccgtacatg gcggcggcgt cactttcgga
     3601 ccttatctgg gtgaaacgtt cgccctggtc gaagcaaaag gcgcagaagg tgcaaaagtc
     3661 tataactcca gtcagctgga aattaatgac agtggttatg cgcttgttcc ggcagtaacg
     3721 ccctatcgct acaaccgtat atctctcgat ccacaaggaa tggatggcga tgccgagctg
     3781 gtcgacagtg aaaaacaggt agcaccggtt gcgggtgcgg cggtgaaagt agtcttccgt
     3841 acccgtcctg gtaaagcgct gctgattaaa tcccgcatgg cagatggttc ggaactgcca
     3901 atgggagccg atgtgctgga tgagaataat actgttgtcg gtatagccgg tcaggggggg
     3961 caaatttacc tccgcacaga acagacaaaa ggccatttgt cagttcgctg gggtgaaggc
     4021 gctaacgata gctgccagtt gccctttgat atcagcggga aggacagcaa tagccctatc
     4081 atccgcctga atgaaacctg tcagtcttga tacagagaaa acccgatgtc taaatcgact
     4141 tttttacaca ttctgattag ctccatcata ttggtggcac taatacaatc atccgcctgg
     4201 gccacttgtt ctaatacacg tgtcggacaa acggaggacg gaagatcggc actcattgag
     4261 ttcggaaaaa ttaacttgac cgacacctat tttgcgccag taggctcact actcgcgaca
     4321 acggtcgtac cttccactaa ctacacatca ggtggcgcgt caggaagctc cgtactgtgg
     4381 gaatgtgacg caacagattt gccaaacatc tattttctgg tcgcaaccaa tggcgacgac
     4441 cgcgtgggtg gctttcacaa tgcaggcggg cctgatggtc tgagtgatgt ctatgccacc
     4501 tggtttgctt ttgtcggtct caagcagact atggcgggcg tgacgattgg tcgttactgg
     4561 aagaaagtgc ccatcaccag ttatgccact cagggaacta aaatccagat ccgcttacag
     4621 gatatccctc ctcttcatgc tgagctttat cgtattagta cgctgcctga taccgctggc
     4681 gcaaccagtt actgcggtaa taccaatgct gatggcgacg gtgtggggta tgcgaaaccc
     4741 tccggcacac cttataactg tacccagcct aatgcctata ttcagctttc cggtaccagt
     4801 ggcattccat ttgctcatga tgaagtgggc gaggacagtg cggatcacta tgatttttgg
     4861 ggcggtgata atggttttgg ttacggaatg cgatcggtga atcgacttta caacaatgcc
     4921 acctgcgttg cccgcagcgc cacgccgtta gtattgctgc cgacaattgc agaagcacaa
     4981 ctgaatgcag gcatggaaag taccgctaat tttaatgtcc gcgtcgagtg tagtaactca
     5041 gttcaatcag ggattagtga tactcagaca gcattaggaa tccaggtgtc tgaaggtgca
     5101 tatacagcag cgcaaaaact ggggattatc aatagcaacg gcggcgtcag cgccttagtc
     5161 tcagataatt atgacgcggc agagatggca aaaggcgttg ggatctatat ttctaacagt
     5221 gctcagcccg atacggagat gacgctggtt ggtcaacctg gcatcgcaaa gttaaccccc
     5281 ggaggaaata ccgcgggatg gtatccagtc tttgaagggg caacattaga aggtgcgacg
     5341 caccccggat actccggcta tagttactct tttatcgccc ggttgaagaa actgccaaat
     5401 cagacagtca gtgcgggaaa agtgcgggca acggcttata tattggtgaa aatgcaatga
     5461 aatccgtttt gctcctctta accgtcttgt tcgtacagtc agtggaagcc ggcatcatgc
     5521 cggcttcttc acggattatt tatcgcgatg gcgatcggga aaagacgctg atgctggtca
     5581 ataccaatga ctatccggta ctggtgcaaa gttgggtaga taatggtgaa ggttccccag
     5641 aagctgcttc tccccccttt gtggtgctgc caccggtttt ccgcctggct gcgagacgaa
     5701 cccaggggtt gcggattatc tataatcatg acccacttcc ccaggatcgc gaatctgcat
     5761 tctggttaaa tctttatgaa atgccaccag aaactcccca tcaagatccc acacatttaa
     5821 cgctggcaat gaatacgcaa cttaaaatct tttacaggcc aaaatctgta actatgacgc
     5881 cccaagaagc tatgcgtaaa ataagttggc atctggttaa caacggcagc agcagctata
     5941 ttgagcttaa taacccaaca ccactgaata tctctttcac ctccatcagc attggggcta
     6001 cgaaggtaga gcaagaaagc gatatgatga taaaaccctt cagtcaacga cggtactggt
     6061 tagcaagtaa aaacgatagt gggatcgtta ctgcgacata tctcgatgat gatggtgcac
     6121 agataaagca acaattagcg caataatacc ccgcgtggat atgtaaactc ttcgcgatag
     6181 ttcaccacta acgaacaaca tctatacccc tgtccccccg aagtggagaa atgacaaaat
     6241 ctctccactc cattttccag ttaaaacacc tcaacgcagc caaaaatcgt taacggattg
     6301 aatatccgcc atcaatgact aatagttgtc cattgacata atctgaagcg gcactggcga
     6361 gaaacaccat tgcgcccata agatcctgtg cccttcccca gcgttgagcc ggtatacgcc
     6421 cttttattaa ctcttttatt ttagggttat tataaataat cgctgtcatt tcactcacat
     6481 aatatcctgg ggcgatacca ttgatctgaa tattatattc cgcaaaatca tcggcatacg
     6541 ctttggtcaa ccccgctaaa gcatgtttag tcgcagcata ccctggagac caacgtcccc
     6601 catgaaatga caatacggaa cacatattaa taattttccc ttttctttgc gggacaaata
     6661 tattcaatgc ggcctggctc atatcgaatg cagcattcag gttgagatta atcatcggat
     6721 cccagtcgtt tcgattgaaa tcgataggac gattgatgtt acacacaccc gcgttattga
     6781 ccagaatatc taactgaccg aaacgttcca gacacctatt aataatggtg cttgttgcat
     6841 caggttttgt tatgtctatt gtcataaact caacttcaac gccttcatga gttaaatgct
     6901 ttaaatattt attatcaggc tctttgatgt ctaccaacaa cagattagct cctgctttgg
     6961 ccagagcaga gcataagcct ctccccagtt cacccgcggc cccagttacg atggccacct
     7021 tgttatgtaa agaaaaaaaa tccaatgaaa aatcttcaat agaaaagct
//
LOCUS       DAPGRN010000181         7060 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPGRN010000181 DAPGRN010000000
VERSION     DAPGRN010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.183"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            61..618
                     /locus_tag="RHW33_004386"
     CDS             61..618
                     /locus_tag="RHW33_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1647821.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSAGDTLVVWKLDRLGCSMRHLVILVEELRECGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVELTRAGFAAARQEGRNGGRKPKLTVEQWEQAGRLIESGIY
                     QQQVALIYDLGISTVYKKFPVASKP"
     gene            complement(645..1340)
                     /locus_tag="RHW33_004387"
     CDS             complement(645..1340)
                     /locus_tag="RHW33_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDV1647822.1"
                     /translation="MKKEILKALCPTCGGLRNCTVHGKLTTSWEDSQYPVYGYHYHHL
                     LQCNGCDTVFYHHNEHFSEHTTHEYRDGEFVETPIDMITTYPAAETFQAPIWLSKLES
                     VDRQLFQIFNEMYSSYTSDHFILSSIGLRTIFDRTAELLQIHPGLPLGEKVEKLKQDG
                     VIGDTEACVISSVIDAGNAAAHRSWSPNKSEFEQMLEAIESFVQRTILGKRSLEHITK
                     QLPPRVTRPKNNA"
     gene            complement(1689..1937)
                     /locus_tag="RHW33_004388"
     CDS             complement(1689..1937)
                     /locus_tag="RHW33_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDV1647823.1"
                     /translation="MRIEICIAKEKMTKLPNGAVDALKEELTRRISKRYDDVEVLVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            2157..3743
                     /gene="prfC"
                     /locus_tag="RHW33_004389"
     CDS             2157..3743
                     /gene="prfC"
                     /locus_tag="RHW33_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV1647824.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            4136..4741
                     /gene="osmY"
                     /locus_tag="RHW33_004390"
     CDS             4136..4741
                     /gene="osmY"
                     /locus_tag="RHW33_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV1647825.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            4868..5029
                     /locus_tag="RHW33_004391"
     CDS             4868..5029
                     /locus_tag="RHW33_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV1647826.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            5151..6224
                     /locus_tag="RHW33_004392"
     CDS             5151..6224
                     /locus_tag="RHW33_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV1647827.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            6221..7003
                     /locus_tag="RHW33_004393"
     CDS             6221..7003
                     /locus_tag="RHW33_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1647828.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVLALAENYKPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEHAARVFAVLCELRREPEDEIAEVLLNNTYAVFSVSG"
BASE COUNT         1774 a         1648 c         1848 g         1790 t
ORIGIN      
        1 tgaaaatgca gaaataacga gcaaatggca tcattcctgc ttttgtcagg gagatctacc
       61 atgcttattg gctatgtacg tgtgtcaaca aatgaccaga acacagatct acaacgtaat
      121 gcgctgaact gtgcaggatg cgagctgatt tttgaagaca agataagcgg tacaaagtcc
      181 gaaaggccgg gactgaaaaa actgctcagg acattatcgg caggtgacac tctggttgtc
      241 tggaagctgg atcggctggg gtgtagtatg cggcatctgg tcattctggt tgaggagttg
      301 cgcgaatgtg gcgttaattt tcgcagcctg acggatgcta ttgataccag cacaccaatg
      361 gggcgctttt tctttcatgt gatgggtgcc ttggctgaaa tggagcgaga gcttattgtc
      421 gaacttacta gggcgggctt tgccgcagca cgacaagaag ggaggaacgg tggacgaaag
      481 ccaaagctga cagtagaaca atgggagcag gctggcaggt tgattgaatc gggaatatat
      541 cagcagcaag tcgcactgat ttatgatctg ggtatttcaa cggtgtataa aaaattccct
      601 gtagcaagta aaccataacc ttacgccata ttgatgatcg cggattatgc attattttta
      661 ggtcttgtaa cccgaggtgg gagttgtttg gttatatgtt caagtgatct tttgcccaag
      721 atagttcttt ggacaaaact ttctatagct tctagcattt gttcgaactc acttttattc
      781 gggctccaac tgcgatgtgc ggcggcgttt cctgcatcaa ttactgaaga tataacgcat
      841 gcctcagtgt cgcctatgac tccatcttgt tttaattttt caactttttc accaagcggc
      901 aaaccggggt gaatttgtaa cagttcagca gttcgatcaa aaatcgtccg taaaccgatg
      961 gacgaaagaa tgaaatggtc agaagtatat gaagaataca tttcattaaa tatttgaaag
     1021 agctgacgat caactgattc aagcttggat aaccatatcg gagcttgaaa tgtttcagcg
     1081 gcaggatagg tagtgatcat atctataggc gtttctacaa attcaccatc acgatattcg
     1141 tgggtagtat gctcactgaa gtgttcgttg tggtgataga agacagtatc acagccattg
     1201 cattggagta ggtgatggta atggtaccca taaactggat attgggaatc ctcccagcta
     1261 gtggttagct tcccatgcac agtgcagtta cgtaacccac cgcatgtagg gcaaagagcc
     1321 ttgagtattt cctttttcat aaagtctctg attctatatg taggttaaga ccactattag
     1381 ttatacggac ctaataatca atcgtgtgca gatacaaaaa agcccgtata gcgggatttc
     1441 atgtcactaa gggccgcggc tactttgcgt atcttttttg tcttctcacc gtctggccgg
     1501 tattttgctg agactgctta tttccagttt ttactagtgc tgtactagta ctgcccaatc
     1561 atgattggtg ggggacggag ttgagaccgc agccacgtcg tatgcaagaa cgcgctgcgg
     1621 ttggctggtg aactttcgat agtgcgagta ttgaatgatt tccagccgtt acagatttta
     1681 cgtgtttatt agtgaacaaa ccactcgtca gcagattccc aagtatcttt cagagtctcc
     1741 tgaacaaaag tttttgcaga atccttatct gcggtgcgtg taacagaaag gccatcattg
     1801 ctggtggctt ttactaacac ctcaacatcg tcataacgct tactgatgcg tcgggttaat
     1861 tcttccttta gcgcatccac agcaccgttt ggcagtttag tcattttctc tttggctatg
     1921 cagatctcaa tacgcataaa aactccttta tactgtgttt gtatacagca tcatttttaa
     1981 ctgtatgaat aaacagtgtc aatagagtaa agattgctgc atgtacaaat gttctttggc
     2041 tacaaataca cctactgatc atgttctgct atgctctgat acctccaaaa tgtacaatca
     2101 gcggcatgaa tttttctttg tacatatgat tgtacataat caaagagaca acaattatga
     2161 caagcctttt ggccgcagag gtagcgaaaa gaagaacctt tgcaattatt tctcacccgg
     2221 acgccggtaa gactaccatc accgagaagg tgctgctgtt cggacaggcc attcagaccg
     2281 ccggtacagt aaaaggccgt ggttccaacc agcacgctaa gtcggactgg atggagatgg
     2341 aaaagcagcg tgggatctcc attactacgt ctgtgatgca gtttccgtat cacgattgcc
     2401 tggttaacct gctcgacacc ccggggcacg aagacttctc ggaagatacc tatcgtaccc
     2461 tgacggcggt ggactgctgc ctgatggtta tcgacgccgc aaaaggtgtt gaagatcgta
     2521 cccgtaagct gatggaagtt acccgtctgc gcgacacgcc gatcctcacc tttatgaaca
     2581 aacttgaccg tgatatccgc gacccgatgg agctgctcga tgaagttgag aacgagctga
     2641 aaatcggctg tgcgccgatc acctggccga ttggctgcgg caagctgttt aaaggcgttt
     2701 accaccttta taaagacgaa acctatctct atcagagcgg taaaggccac accattcagg
     2761 aagtccgcat tgttaaaggg ctgaataacc cggatctcga tgctgcggtt ggtgaagatc
     2821 tggcacagca gctgcgtgac gaactggaac tggtgaaagg cgcgtctaac gagttcgaca
     2881 aagagctgtt ccttgcgggc gaaatcactc cggtattctt cggtactgcg ctgggtaact
     2941 tcggcgtcga tcatatgttg gatggcctgg tggagtgggc acctgcgccg atgccgcgtc
     3001 agactgatac ccgtaccgta gaagcgagcg aagataaatt taccggcttc gtatttaaaa
     3061 ttcaggccaa catggacccg aaacaccgcg accgcgtggc gtttatgcgt gtggtatccg
     3121 gtaaatatga aaaaggcatg aaactgcgcc aggtgcgcac cgcgaaagat gtggtgatct
     3181 ccgacgcgct gacctttatg gcgggtgacc gttcgcacgt tgaagaagcg tatccgggcg
     3241 atatcctcgg cctgcacaac cacggcacca ttcagatcgg cgacaccttt acccagggtg
     3301 agatgatgaa gttcaccggt attccgaact tcgcaccaga actgttccgt cgtatccgcc
     3361 tgaaagatcc gttgaagcaa aaacagctgc tcaaagggct ggtacagctt tctgaagagg
     3421 gcgcggtgca ggtgttccgt ccgatctcca acaacgacct gatcgttggt gcggttggtg
     3481 tgctgcagtt tgatgtggtg gtagcgcgcc tgaagagcga atacaacgtt gaagcagtat
     3541 atgaatcagt caacgttgcc accgcccgct gggtagaatg cgcggacgcg aagaaattcg
     3601 aagagttcaa gcgtaagaac gaaagccaac tggcgctgga tggcggcgac aacctcgctt
     3661 acatcgctac cagcatggtc aacctgcgcc tggcgcagga acgttatccg gacgttcagt
     3721 tccaccagac ccgcgagcat taattcttac ctccagggcg cggtagccgc tgcgccctgt
     3781 caatttccct tccttattag ccgcttacgg aatgttctta aaacattcac ttttgcttat
     3841 gttttcgctg atatcccgag cggtttcaaa attgtgatct atatttaaca aagtgatgac
     3901 atttctgacg gcgttaaata ccgttcaatg cgtagatatc agtatctaaa gccgtcgatt
     3961 gtcattctac cgatattaat aactgattca gaggctgtaa tggtcgttat tcatcaccca
     4021 tcgcttttgt gatcgcgacc attgacttct gtagagggtg aagtctctcc ctattcagca
     4081 atgcaacctc gtgttgccag gctcaaatta cgagcaaaca tacaggaata aatcgatgac
     4141 tatgacaaga ctgaagattt cgaaaactct gctggctgta atgttgacct ctgccgtcgc
     4201 gaccggctct gcctacgcgg aaaacaacgc gcagactacc aatgaaagcg cagggcaaaa
     4261 agtcgatagc tctatgaata aagtcggtaa tttcatggat gacagcgcca tcaccgcgaa
     4321 agtgaaggcg gcgctggtgg atcatgacaa catcaagagc accgatatct ctgtcaaaac
     4381 cgatcaaaaa gtcgtgaccc tgagcggttt cgttgaaagt caggcccagg ccgaagaggc
     4441 agtgaaagtg gcgaaaggcg ttgaaggcgt gacctctgtc agcgacaaac tgcacgttcg
     4501 cgacgctaaa gaaggctcgg tgaagggcta cgcgggtgac accgccacca ccagtgaaat
     4561 caaagccaaa ctgctggcgg acgatatcgt cccttcacgt aaagtgaaag ttgaaaccac
     4621 cgatggcgtg gttcagctct ccggtaccgt agattctcag gcacaaagtg accgtgctga
     4681 aagtatcgcc aaagcggtag atggtgtgaa aagcgttaaa aatgatctga aaactaagta
     4741 attcgtcgta attcgtcctc ccgaactttg tcgggaggcg cgatgtgcac cacacttaaa
     4801 atatcgcatt gagtgatttt cacgctcata tcaagcggtg acattaacta tggtaaagga
     4861 gacgcttatg tttcgttggg gcatcatatt tctggttatc gcgttaatcg ccgccgcact
     4921 tgggtttggt ggtctggccg gtaccgctgc aggcgcagct aaaattgtct ttgtcgtcgg
     4981 gattattctg ttcctggtga gtttgttcat gggccgaaaa cgaccctaga tttcagacca
     5041 ttaagaaaat tttatccaaa gccagtccag cggactggct tttgcggttt tagcgaataa
     5101 taaattgcgt tactttgtct ttctttcaca acaaaaacag gaaggcagaa gtggggcagc
     5161 gaatacctgt aacgcttggt aatattgcgc cgttgtcgct aaggccgttc cagcctggac
     5221 gaatagctct ggtgtgcgaa ggcggcgggc agcgtggaat tttcacggct ggcgtgctgg
     5281 atgagtttat gcgcgcgcag tttaatcctt tcgatcttta tctcggcaca tctgccgggg
     5341 cgcagaacct ctcggcgttt atctgcaatc agcccggtta cgcgcgcaaa gtcatcatgc
     5401 gctataccac aaaacgcgaa tttttcgatc cattgcgctt tgtccgtgga ggaaatctta
     5461 tcgatctcga ctggctggtg gaggccaccg caagccagat gccgttgcaa atggataccg
     5521 ccgcgcggtt gtttgacagc ggcaaatcgt tttatatgtg cgcctgtcgt caggatgact
     5581 acgcgccgaa ttacttttta ccaaccaaac aaaactggct ggatgtgatt cgcgcctcca
     5641 gtgcgatacc tggcttttat cgtagcggag tgtcgctgga aggcattaac tacctggatg
     5701 gcgggatcag tgatgcgatt ccggttaaag aggcggcaag gcagggcgct aaaacgttag
     5761 tcgtcatccg cactgtgccg tcgcaaatgt actatacgcc gcagtggttt aaacgcatgg
     5821 aacgctggct gggcgacagt agcctgcaac cgctggtcaa tctggtacag catcatgaaa
     5881 ccagctatcg tgacattcag caatttattg agaaaccacc gggcaagctg cggatattcg
     5941 aaatttatcc gccgaagccg ttgcatagta tcgcgctcgg cagtcggatt ccggcgctgc
     6001 gtgaagacta taaacttgga cgtttatgcg gtcgttattt tctggcaacg gttggtaagc
     6061 tattaactga aaaagcgccg cttacccgcc atctggtgcc ggtggtgacg ccggaatcga
     6121 tcgttattcc gcctgcgcca gtcgccaacg atacgctggt tgccgaagtg agcgacgctc
     6181 cgcaggcgaa cgacccgaca tttaacaatg aggatctggc ttgatttgcc gttttatcga
     6241 cacccactgc cattttgatt tcccgccgtt tagtggcgat gaagaggcca gcctgcaacg
     6301 cgcggcacaa gcgggtgtag gcaagatcat tgttccggcg acagaggcgg aaaattttgc
     6361 ccgtgtgttg gcattagcgg aaaattataa accgctgtat gccgcattgg gcttgcatcc
     6421 tggtatgttg gaaaaacata gcgatgtgtc tcttgagcag ctacagcagg cgctggaaag
     6481 gcgtccggcg aaggtggtgg cggtggggga gataggtctg gatctctttg gcgacgatcc
     6541 gcaatttgag aggcagcagt ggttactcga cgaacaactg aaactggcga aacgctacga
     6601 tctgccggtg atcctgcatt cacggcgcac acacgacaaa ctggcgatgc atcttaaacg
     6661 ccacgattta tcgcgcactg gcgtggttca cggtttttcc ggcagcctgc aacaggccga
     6721 gcggtttgta cagctgggct acaaaatcgg cgtaggcggt actatcacct atccacgcgc
     6781 cagtaaaacc cgcgatgtca tcgcaaaatt accgctggca tcgttgttgc tggaaaccga
     6841 cgcgccggat atgccgctca acggttttca ggggcagcct aaccgcccgg agcatgctgc
     6901 gcgggtgttt gccgtgcttt gcgagttgcg ccgggaacca gaagatgaga ttgccgaagt
     6961 gttgcttaat aatacgtatg cggtgtttag cgttagtggg tagtttgccc ttcgtaggca
     7021 gcatccggca gtcggcgcat aatgcctgat gcgacgctta
//
LOCUS       DAPGRN010000182         7035 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPGRN010000182 DAPGRN010000000
VERSION     DAPGRN010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.184"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            205..1056
                     /locus_tag="RHW33_004394"
     CDS             205..1056
                     /locus_tag="RHW33_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1647829.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAITTGYRLID
                     TAASYQNETQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLHAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGGKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
     gene            1083..2072
                     /locus_tag="RHW33_004395"
     CDS             1083..2072
                     /locus_tag="RHW33_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV1647830.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGSIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQITEALETIKIVGERYSPEHQARVGR"
     gene            complement(2104..2997)
                     /locus_tag="RHW33_004396"
     CDS             complement(2104..2997)
                     /locus_tag="RHW33_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1647831.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQQNGTPSGNIRLSAGE
                     HAARSLVWPKLKPFLRKYPEISVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GPDMRMAVVGAPAYFAANSAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKTLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(3198..4124)
                     /locus_tag="RHW33_004397"
     CDS             complement(3198..4124)
                     /locus_tag="RHW33_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1647832.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQRLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKLEN"
     gene            4281..5201
                     /locus_tag="RHW33_004398"
     CDS             4281..5201
                     /locus_tag="RHW33_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV1647833.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTSLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPDAALEAGLTEIDLFEATEYYRSPRGYAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPIMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
     gene            5446..6588
                     /locus_tag="RHW33_004399"
     CDS             5446..6588
                     /locus_tag="RHW33_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDV1647834.1"
                     /translation="MTNKHPSLFSPFMLTEKIKLRNRIVMAPMTTWSANPDGTISEQE
                     LEFYKRRSQNVGLVITGCTYITPSGIGFTHEFAAYDDRFINSLEKLAAAAKSGGAPAI
                     LQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRVVQSREMTENEIQETIRAFGDV
                     TKRAIKAGFDGIELHGAHGFLLQNFFSPLFNQRNDRWGGDLEGRMRFPLAVLQEVKNV
                     VYEYATKPFAIGYRISPEESATGGLRIEDTYKLLDRLISSGISYIHTSLVSINDSYPV
                     ESPNGPRTIELILNHIAGRVPVIAAGKIRTPSQAQEAISTGLPLVAIGKGLVINPEWV
                     TLAESGRGHEIQTTLNPQLVPELTIPDKLWDQIQASKGTGWFPLMD"
BASE COUNT         2006 a         1650 c         1563 g         1816 t
ORIGIN      
        1 gccaggataa accgatatat ttttatggat attcattttc ctttatcgga ctgtaaattg
       61 cggggatatc gaacttgtac tactcatctc aacactcaaa cagatttatg aatttcgttc
      121 ataacccctg acaaatcctg ccgtctaatc gagctcttct ctttacgtta tgcttctcat
      181 gaacgataac ggaaggataa caccatgcaa aaagtaaaac tgaataacgg tattgaaatg
      241 cccctgctgg gctttggtgt atttcagatg accgatgccg ccgaatgcga aagagcggtc
      301 attgacgcta ttaccaccgg ttatcgactg attgacaccg ccgcctccta tcaaaatgaa
      361 acccaggtcg ggaacgcact gaaacaaagc ggtattgcgc gtaacgagct ctttgtcacc
      421 acgaaactct ggttgcagga taccagttac gaaggggcca aagcccagtt cgaacgctct
      481 ctgaatcgac tgcaacttga ttacgttgac ctgtatttga ttcatcagcc ttacggtgat
      541 gtccacggtg cctggcgtgc catggaagag ctgcaacagg ccggtaaaat tcgcgctatt
      601 ggcgtcagta acttccatcc tgaccgactg gctgacctta tcgcctttaa taacgtcgtt
      661 ccagcggtaa accaggttga agttaaccct ttcaatcagc agctacacgc cgttccgtgg
      721 atgcaaagcc gtggtattca gccagaagcc tgggcaccgt ttgcggaagg gaaaaatggt
      781 ctgttccagc atcctgttct cacggcaatt ggcgggaagt atggcaaaag cgtaggccag
      841 gttgttctgc gctggatcta ccaacgaggc attgtttcgc ttgcaaaatc agtacgcaaa
      901 gaacgcatgg aagagaacat caacattctc gattttgaac tcagtcctga agatatgctg
      961 caaattaccg ccctggatac cgcaaccagc gcgttcttct ctcaccgcga tcccgcaatg
     1021 gtggaatggc tgactggccg caaacttgat gtttaagccg cgataaaaga ggtattcatt
     1081 caatgcaaaa acgttatctg ggtaaatccg gactcgaagt ctccgccctt gggctcggtt
     1141 gcatgggctt aagccacggc tacggaccgg cgaccgatac ccgtcaggct atcgaactta
     1201 ttcgtgctgc ggttgaacgt ggcgtcacct tcttcgatac agccgaagtg tatggtccgt
     1261 atctgaatga agaagtggtc ggcgaagcct taaaaccgtt tcgtgaccgc gtggtaattg
     1321 ccactaaatt cggttttacc ttcggcgatg ataataagca gcagatttta aacagccgtc
     1381 cggaacatat ccgtgaagcc gttgaaggat cattacgtcg tctgaagaca gatgttattg
     1441 acctgctgta ccaacaccgt gttgatccgg atgttcctat tgaagatgtt gcaggaacgg
     1501 tgaaagacct catcgcagaa ggtaaggtca aacatttcgg tctgtccgag gctggcgcac
     1561 aaaccattcg tcgtgcgcat gcggtacaac ctgtcaccgc cctgcaaagc gaatattcca
     1621 tgtggtggcg tgagcctgaa caggaaattc tgccgttgct ggaggaactg gggattggtt
     1681 ttgtcccctt cagcccacta ggtaaaggtt tcctgaccgg gtcgattaag ccgggaacca
     1741 ctttcggcaa agatgattac cgcagcaccg taccgcgttt cgcagcacaa gccattgaag
     1801 ctaatgaaaa actggtcacc ttgttaggtg aactggctgc ggagaaaggg gtaacgtctg
     1861 cacaaatcgc actggcatgg ctgttagcac aaaagccgtg gattgttccc atccctggca
     1921 ccactaaact gcaccggctg gaagaaaacc tggccgctgc tgacattgtt ctttcgcaga
     1981 aagatacgca gcagataacc gaggcgctgg aaacaattaa aatcgtcggt gaacgttact
     2041 cgcctgaaca ccaggcacgc gtaggccgct aatacccaaa cgggtccagg aacaaaggac
     2101 ccgttattcg gtgtagcgaa gcgcatcgat taacagcgca aaagcaggcg gatgctgctt
     2161 gcggctgggg tagtaaagat aatatccggg aaaagttgga caccactcct gtaacacctg
     2221 aataagcttg cctgacttta tatactcctg aaccatgtct tcagggacac aagcgatccc
     2281 aaaccctgat aacgcggcat caattctttc tggcaatgga ctacacgtca gctgcccatc
     2341 aaccttaacg cgaagagttt tcccttctcg ctcgaactcc cagtggtaaa gtccaccggc
     2401 agttggcaaa cgcatgttga tgcagcgatg gttttgtagc tcgtgaggcg tttcaggcgc
     2461 agagtttgca gcaaaatacg ccggtgcccc caccacagcc atccgcatat ctggtccaat
     2521 tctgaccgca accatatctt tatcaacatt ttcacccagt cgtatcccgg catcaaaacg
     2581 gccttcaaca atatcgacaa aaccgttatc caccaccagt tcgacactga tttcgggata
     2641 ctttctgaga aaaggtttta gcttcggcca gaccagactt cgcgcggcat gttccccggc
     2701 agataaacga atattcccgg agggcgtgcc gttctgttga ataagtgact ccagttcctg
     2761 ttcaagctcg tcaatacgcg gttcaaggca ggcaatgatt ctctcccccg cttctgtggg
     2821 agccacactg cgggtagtcc gtgtcaggag gcggatattg agcctttcct ccagagcctt
     2881 cattgcatgg ctaagtgcag actgagagac gccgagtttg cccgctgctt tggtaaaact
     2941 tcgctctctt gccaccacaa gaaagatttg cagttcgttg aagttttctt tgagcattgg
     3001 cgatttcctt gtagaaacat cctcttcaca gacactcatg aaggaggggc atagatgcta
     3061 ttattttagc cacattatta gtaacaatga taatctggct gtatcgaaaa taattctaaa
     3121 ctttcaggag atttattatg aataaacgta tcctcacgat tgtgccattc ttcttaccga
     3181 tattctgaca catatcatca attttcgagt tttactcttt tctccaccat aaaatcaata
     3241 aaagcacgaa ctttagaggg cattctcacc cgtgatgaaa cataaatata aaatccagga
     3301 aatgaaacag cccaatcctc aagaatcggc tgtaacttgc ccgcattgat atgttcagcg
     3361 attgcaaaat ccagatgttg aatgataccc gttccattca gcgccattcg tatcatgctt
     3421 tcgtcatcat tagaaatata cttacctgaa acctctattt cgaactcttc attacccccc
     3481 acaacattag aaaactccca tgcagagagt gcaccgctac ttttatgccg caaccgaagg
     3541 caattatggt tactcagttc ctgaggcgtt tcaggaatac cgtgttcttt taaataatct
     3601 ggcgatgcca cagtaaccag tttaataagc ggagaaacag gaacggcagt catatgctca
     3661 tccagtcctt gctcaagacg tatgcccaca tcacagccgt ccgcaataat gttagataaa
     3721 ccgtcatcca taatgagttc tatctttgtt ttggggtaac gagtaagaaa ctcgccaata
     3781 tgaggctcaa caagcatccg tgctgcaaca cgggaggaat ttattctcaa caggcctgta
     3841 ggttcaagtt gggtatcacc aacatctttc aaagcatcat caatttgccc caaagccgac
     3901 accagcactt catacagacg ctgtccttcc tcagtaagcg acatattgcg ggtcgtacgg
     3961 tatatcagct tggtgttaag acgttcctca agagatttta aattctggga caatgcggcc
     4021 cgcgtaatac ccatttcaga agcggctctg gaaaaactgc cggcacgaac aataagcgca
     4081 aaccaggcaa gagaaggcaa aattgaaggc tctattttca tcattgtaaa ccttaactta
     4141 acactgtgat agatattaga ccattgatga taaccaatac aagtcatact attacgccgt
     4201 tctcgatatg aacacgaatg ccagttgcgg ataatagaaa tgatatttaa atctgtttag
     4261 gcaaatcccc ggagcttgaa atgaaaactg tttcgataaa acacacttac tgggatatag
     4321 ctgctgatat ctatttccct gacgatttta atagtgaaaa aaagtatcct gccattatca
     4381 gcgcccatcc aattggaagc tgcaaagaac agacatcggg tagtgtgtat ggtgccgccc
     4441 tggcaaaagc tggctttatc gtcattgctt ttgatgccag ttttcaggga agcagcggtg
     4501 gagaaccacg ttatctggaa gatccaacaa tgcgagtaaa agacttcagc attgtcgttg
     4561 attaccttac cagcttacct tacgttgatg ccgggcgtat tggtgtactg ggtatttgtg
     4621 gtggaggtgg atatgccatt aatgccgcaa tgacagaacg tcgaattaaa gctattggta
     4681 ctgtcacggg tgcaaactac gggcgtctta tgcgtgaggg atttacagcg tttaatccta
     4741 tcggagcact cgaagcaatg gcgcaacaaa gaacagacga agctaacggc gcaaaacttc
     4801 gcgtagatga tttactcccc tcttctccgg atgcagctct tgaagcggga ttaacagaaa
     4861 tagacctttt tgaagccaca gaatattatc gttcgccacg aggctatgca cctaatgggg
     4921 taaatcgttc gctattctca catcagactg ttgccgtggg ttgggatgca tttcatcttg
     4981 cagaagtgct gttaactcaa ccaataatgg ttgtggtggg cgatagagtg ggcgcatttg
     5041 gtgcctaccg tgacggctgc gaaattattg gccgcgctgc ttcaaaacat aaagagctgg
     5101 tcgtggttga aggttattca cattacgacc tgtatgataa gccagaacct gtgaaacagg
     5161 cactcgataa attaattcct ttttataaaa cacatttgta ataattttac agggagattc
     5221 cttatctccc ttatatttat cctttattga caacacaaca ataataaaat tactcaaaaa
     5281 gaaatttcat taaagaaaca aaaaagcagc aactcaatta ttaagattat tagaaaccct
     5341 ttgaagggat tataaataaa gccagaaatc ttgattccaa agctggcaac aactaaccag
     5401 ttaaaacata cgcatcaaaa tcagataaac atttgaggta ttgttatgac aaataaacac
     5461 ccatcattgt tttctccttt tatgctgaca gaaaaaataa agctacgtaa tcgtatcgtt
     5521 atggctccta tgacgacctg gtcagcgaat cctgatggaa ccatctctga gcaagaactc
     5581 gaattttata aacgtcgctc acaaaatgtt ggtttagtaa taacaggatg tacctatatc
     5641 acgccaagcg gcattgggtt tactcatgaa tttgccgcgt atgacgaccg tttcattaac
     5701 agccttgaaa agttagcagc agcagccaaa agtgggggtg ctccggctat tttgcagatt
     5761 ttccacgccg gtaataaagc catcccggag cttgtaccaa ataacgatgt tattagtgcc
     5821 agcgcatcaa gcgtaaaatc tggtgacttt atgaagagag tggtacaaag cagggaaatg
     5881 actgaaaacg aaattcagga aaccattaga gcattcggag atgtcacaaa aagggcgatt
     5941 aaagcaggtt ttgatggcat tgaacttcat ggtgcgcacg gattccttct acagaatttc
     6001 ttctcccctc tatttaacca gcgtaatgac cgatggggag gagatctcga aggacgtatg
     6061 cgcttcccgc tggccgtatt acaggaagta aaaaatgtag tctatgaata tgcgacaaag
     6121 ccctttgcca tcggttacag aatatctcct gaagaatctg caacaggggg gctgcggata
     6181 gaggatactt ataaactcct tgatcggctc atttcatccg gtatttcgta tatacatacc
     6241 tcgctggtca gtattaacga tagctaccct gttgagtcgc caaatggccc ccgaactatt
     6301 gaattgattt taaatcatat tgcaggcaga gtgccggtaa tcgctgcagg taaaatacgc
     6361 acaccgtctc aggctcaaga agcaatttcc actggcctgc cacttgttgc gatcggtaaa
     6421 gggctggtga taaatccaga atgggtgact ttagctgaat caggtagagg ccatgaaatt
     6481 caaacaaccc ttaatccaca acttgttcct gaattaacca ttccggataa actatgggat
     6541 caaattcagg catcgaaagg aactggctgg tttccgttaa tggattaaat atctattact
     6601 ctcccgagag taaaactatc aaatcaatga gtaaccgata cagcctcaca aattctttca
     6661 gataattaac ccgtcactgc ggcacgttct ctcataaggc gtgcctgcag tgtaatccag
     6721 tatgtatccg gcatagttga cggtttaata gagctcaatt cctcgcccag gttaccaaca
     6781 acgatatctt gtataacatc ccaggagata cgacattatg atgagatgcg atatgctcct
     6841 cagcgccaat caacgccagg actgggccag tcttgagatc agactcattc ccggaactga
     6901 cttcaggcga catatatcaa tgacgttggt gcaataaaag atagccgatg taatacatct
     6961 attcaaattt gtgatacatg tcaaaaaatt ggttgactaa actcgttatt tatataaggg
     7021 cacttacgaa gtgcg
//
LOCUS       DAPGRN010000183         6941 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPGRN010000183 DAPGRN010000000
VERSION     DAPGRN010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.185"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(107..886)
                     /gene="istB"
                     /locus_tag="RHW33_004400"
     CDS             complement(107..886)
                     /gene="istB"
                     /locus_tag="RHW33_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDV1647835.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            complement(886..1907)
                     /gene="istA"
                     /locus_tag="RHW33_004401"
                     /pseudo
     CDS             complement(886..1907)
                     /gene="istA"
                     /locus_tag="RHW33_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            2656..3222
                     /locus_tag="RHW33_004402"
     CDS             2656..3222
                     /locus_tag="RHW33_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV1647836.1"
                     /translation="MLKSYTPNWFFTALLDNHINQLVARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTVDHGFHAHAVFWIDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKLHF"
     gene            3481..4056
                     /locus_tag="RHW33_004403"
     CDS             3481..4056
                     /locus_tag="RHW33_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647837.1"
                     /translation="MNAIPYFDDSLAPFWPSYQSKVIGVLERALREQSGSRIRRILLR
                     LPWEHDNAFNSRQTWFGMDFIETVSALMNAKPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAAPYQKQITRDVVLDSRSPDCEDILKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPGYPGSAGPF"
     gene            4125..4361
                     /locus_tag="RHW33_004404"
     CDS             4125..4361
                     /locus_tag="RHW33_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV1647838.1"
                     /translation="MLTSMTGHDCVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            4358..4516
                     /locus_tag="RHW33_004405"
     CDS             4358..4516
                     /locus_tag="RHW33_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647839.1"
                     /translation="MKRVAMSALVLRKSALSLVLATLPFIFLLTLSGQPADCSSFLTD
                     SSFHSHCL"
     gene            4604..5152
                     /locus_tag="RHW33_004406"
     CDS             4604..5152
                     /locus_tag="RHW33_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647840.1"
                     /translation="MQITEALISEPGDIRRFIQQAVDHWPHLLAFYFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEECSQVVDLLQQSWQVISAGGQCRVEKRFQVARGDTSGQYVALKTVALSL
                     GLPVVTAIIYRPVQRCTLITAQ"
     gene            complement(5171..5515)
                     /locus_tag="RHW33_004407"
                     /pseudo
     CDS             complement(5171..5515)
                     /locus_tag="RHW33_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prop effector"
     gene            5821..5994
                     /locus_tag="RHW33_004408"
     CDS             5821..5994
                     /locus_tag="RHW33_004408"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647841.1"
                     /translation="MARPVGSLNKRTISANTTVPGEVKSFLLAQFLKKWRRTKMGRHT
                     WKVSLPDDAKKQK"
     gene            6408..6842
                     /locus_tag="RHW33_004409"
     CDS             6408..6842
                     /locus_tag="RHW33_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV1647842.1"
                     /translation="MSKKKISVENEEFITYRALLLSIDFVKKLVTEMSDEDLNKVKEN
                     FDIAIQQRDERKENMKAIKSLMDEHGISLEDFYREFGKKSQAGRKSVSGGHKYEYDDN
                     GKRMYWNGQGRRPKAIAEAIKAGGSLDDFLISQEVSSGPDYE"
BASE COUNT         1747 a         1599 c         1675 g         1920 t
ORIGIN      
        1 cttaacgatg attgtttatc tgaagtgttt taactattct tgtcaacgac ggatgaaaag
       61 tgatccactt atatctccac caacggccca atattgatcc accgttttac tcaggattag
      121 cttctgctat aaccccggcc tttcgtttct gtctgagtcg atagctttct cctttgattt
      181 gaacgacatg tgagtggtgt aagatacggt ccagcatcgc tgaggtcagt gctgcatcac
      241 cggcgaacgt ttgatcccac tgcccgaacg gcagattgga tgtcaggatc attgcgctct
      301 tttcgtaacg tttagcgatg acctggaaga acagctttgc ttcttcctga ctgaacggca
      361 gatagcctat ttcatcaatg atgagcaggc ggggggccat tactccacgc tgaagcgtcg
      421 ttttataacg gccctgacgt tgtgccgtag ataactgaag taacagatct gctgctgttg
      481 tgaagcgaac tttgatacct gcacggacgg cttcatagcc catcgctatt gccagatggg
      541 ttttccccac acctgatggc cccagtaata cgatattttc attacgttct atgaagctga
      601 gtgagcgtaa cgactggagt tgcttctgcg gtgctccggt ggcgaatgtg aagtcatact
      661 cttcgaacgt tttcaccgcc gggaaggctg ccattcgggt atacatcgcc tgtttacgtt
      721 gatgacgtgc cagtttttct tcatgaagca gatgctccag gaagtccata taactccatt
      781 cctggtctac tgcctgttgt gacagcgcag gcgctgcgct tataaggctt tccagttgca
      841 actgcccggc gagcgccatc agtcgttgat gttgcagttc catcatcacg ccactcctct
      901 gcagaatgag tcgtagatgg agagtggatg atgcaggggg tgtttgtcga agttcaccag
      961 attttcacca ggatgcacgt catactcttt tttctccgga ggcagtgcca gcatggactg
     1021 ctgctcttcg agccagcgat cgcagggacg tgcctggatt gtttcatgct ttcgttggtt
     1081 agcgacatcg tgcagccagc gcagaccgtg gcggttggct gtttcaacat cgacagtgat
     1141 caccatcggg cgcaggcgag tcattagtgg gatgtaaaaa ctgttacggg tgtactgcac
     1201 catccgttcc accttacctt tagtctgtgc cctgaagggg cgacacagtc ggggagagaa
     1261 gcccatctcc ttgccgaact gccacagcga aggatggacc ggtgctgacc ggtctgatat
     1321 gcgtcacgtt gcagaaccac agttttcata ttgtcataca acacttcgcg cggcacacca
     1381 ccaaagaagc ggaacgcatt acgatggcag gtctccagcg tgtcataacg catattgtca
     1441 gtgaattcga tgtacagcat tcggctgtat ccgagaacag caacgaacac gtgaagcggt
     1501 gagcgaccat tacgcatagt gccccagtca acctgcatct gtcgtccggg ttcagtttcg
     1561 aaccgaacgg caggctcctg ctcctgagga accgagagag aacgaatgaa tgccctgaga
     1621 atggtcattc cgccacgata tccctggtct ctgatctcgc gagcgattac cgttgccggg
     1681 attttgtaag gatgagcatc ggcgatgcgt tgacgaatat aatcccggta ttcatccagg
     1741 agtgaagcaa cagcaggtcg cggcgtatat tttggcggct cagattttgc ctgcaaataa
     1801 cgtttaacgg tattgcggga gatccccagt tctctggcaa tcgcccggct actcattccc
     1861 tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataaa ctctcctgaa
     1921 tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga tcactattgc
     1981 accgttcgtg acaattctgg ttggaaacaa taattctgtt atcactgatt aaccgaatgc
     2041 ttactctttg ataaggtatc catgattctg taacgattaa cagaactgga taaacaacag
     2101 aatcaacaga gtgaatttct gattatgttt gttcgtttct gactatcttt tatgttaagc
     2161 aagataaaaa taatccatgg tatattttat tctggtatcg tgtgccgtta acgctcatgt
     2221 tttgaagtga ttcgttttta acaaattaaa tgagagtgta taattttatc aggttataga
     2281 tatgtgttgg ccttgtacac ggtttttaca accactgaga gcaagtttgt agtttatgat
     2341 attattggtc gtaatatgtt tcttaatctc ctggtagtgg gtttttatcg cgtgttttgc
     2401 agtatttcgt gatgtttatt gattctgtat tttattcgct cctcgtttat ctcatctctt
     2461 tagttaatac catcagataa tccatttctt tctgcataat gctgcgtatc gttaataact
     2521 cgtcgtatcc attctgctac agcatgcctg ataaatacca tctgtaagtt attaccgttt
     2581 tagatctgat tatgagcgaa agcattaatt cgctcacaga gcttaaaaca tcattaactt
     2641 tcaggagtca tcaacatgct taaatcttac acaccaaact ggttttttac cgctttactt
     2701 gacaatcaca tcaatcagtt ggtcgcacgc tattcctgcc tgcgggcctt acggatggat
     2761 ttcttctaca ggaaagatac gcctgatttc ttacaacctg atcatcgctg gcttgaattg
     2821 cagttgcgta tgctgctgga gcaggtggaa caatttgaaa atatcgttgg cttcttctgg
     2881 gtgattgaat ggacggttga tcatggtttc catgcgcatg cggttttctg gatcgatcgt
     2941 cagagggtta aaaaaatata tccctttgcg gagcggatta cggaatgctg gcggtctatt
     3001 acgcataaca gcggttcggc acaccgctgt acatatcagc cgcattatac atacaacatc
     3061 aacattcctg tgcgccacaa tgatcctgaa agcatcgata atattcgcgg tgccctgcat
     3121 tatctggcga aagaagaaca aaaagacggg ctgtgtgcct acggctgcaa tgaagttcct
     3181 gaacgtcctg ctgcaggacg accacgtaag cttcatttct gaaagtgtaa cgcttaagcc
     3241 ttctgttctg gaaacactcc gtcggtaaaa acttaccgcc ttgattaatg atgtgaaccg
     3301 aagtcaacgg agatcattca tcctgaacct gtatccggtg ttcgcccctt gtcggtgacc
     3361 tgatcttccc gttctgtttc ggttcttcac gtattccatc aaccccatcc gcaaaccata
     3421 acacgtcagc tcattcacgg gcaggacgcg ttgtgggctg cgcataacgg agcatatctt
     3481 atgaatgcta ttccttattt cgacgatagc cttgccccct tctggccatc ttatcagagc
     3541 aaagtcatcg gcgtccttga gcgtgcgctg cgtgagcagt ccggctcacg gatacggcgg
     3601 atcctgcttc gtctgccgtg ggaacatgac aacgccttca acagcagaca gacctggttt
     3661 ggtatggatt ttatcgaaac cgtcagtgcg ctgatgaatg cgaaacccgg acgcgacctt
     3721 tgctggctcc tgacccgtca tcctgaaaag ccggaatacc acgtggtgct gtgtgtcaga
     3781 caggagtatt tcgacggccc tgaactggac cggctgattc tggatgcctg gagcaacgta
     3841 ctgggcttcg cgtcaccggg ggaagcggca ccgtaccaga aacagataac ccgggatgtg
     3901 gtgctggaca gtcgttcacc ggactgcgaa gacatcctca aagatttgat ctgggcgttc
     3961 agtgatttcg cccgcgatcg tcgtggtgtg tgcgatccgg aagcccgttg cctcgccggt
     4021 aatcccggct atcccggatc tgcaggaccg ttctgagaat acggctcagc atctttcccc
     4081 gcattcgcac aaccttcata aatctcacgg aggatattct gaccatgttg acctcaatga
     4141 caggccacga ctgcgtgttg ctgcgtgccg acgatcccct gatcgacatg aactacatca
     4201 ccagtttcac cggtatgaca gataaatggt tttacaagct gatcagtgaa ggccatttcc
     4261 cgaaacccat caagctgggc cgcagcagcc gctggtacaa aagtgaagtg gagcagtgga
     4321 tgcagcagcg aattgaggaa tcacgaggag cagcagcatg aaacgtgttg caatgtcggc
     4381 gctggtattg cggaaatcag ccctgtccct ggtgctggcg acattaccgt tcatcttcct
     4441 gctgaccctc tccggtcagc cagcagattg tagctctttc ctgactgatt cgtcattcca
     4501 ttcacattgt ttataactgg cattacaccg gtgttgatgc gcaccttccc gtgtgtctgc
     4561 accggctgaa caaaattcaa cagggtttga aaaggaacat ttcgtgcaaa taaccgaagc
     4621 attaatttca gagccgggag acatccggcg ttttattcag caggcggtgg accactggcc
     4681 gcatttgctg gcattttact tcacactcca ttcggcagaa ggaaatatca acgggcaaca
     4741 gattcatgca ttctgtactt ccttttaccg acaggtgcac gaacgtatta ctgagcgtaa
     4801 tcacacggcc agtccatcct cgccggtggt attacgctgg ttgcgggaac aacatggagg
     4861 agcaacaata cgatgcctgt tgctgttcag ccaggagctt ttctgccatc cgcgagccag
     4921 tgtgacagtt gatgaagaat gttcgcaagt ggtggattta ctgcaacaga gctggcaggt
     4981 gataagtgct ggcggacaat gccgggtgga aaagcgtttt caggtcgccc ggggtgatac
     5041 atctggccag tatgttgcgt taaaaacagt cgcattgtct ctggggttac cggttgtgac
     5101 cgccattatc tatcgtccgg tacagcgctg tacattgatt acagctcagt gaatcagcgc
     5161 agttgtgtag tcaacgaggt tttctggctt tttgtcggtc attcagcacc attagccggg
     5221 cctctgcctc tttctgttcc tgtagtgtca cttccccgca cggcagcccc ttcagatcgt
     5281 aacgggcgcc atctgccatt aacgcacatt aatagtgcgg agactgcaca taagacgcca
     5341 ctgccgcgtg taatgtacca gcgccaaacg ccagttctct gatggcgata tcctgtatca
     5401 ggtcgtcagg tatctcaact ttaagcggtt ggggtttttc ccggttgaat aactcaggcc
     5461 agagttccac aagacgatta atacgcttac ggtttttacg ctggcgtttt gtcatatgcc
     5521 gccacggtgt tttttcagca gagcagggct ctgtttatcg ttcaactgtg gtatccgttc
     5581 cctggccttc acgttgtgtg cagtgttcgt tttatcctgc agcggcgttg ccggtgattt
     5641 actgaaaatg ccctgtggtt tacggttaat ggtcagcgtt accatcggta ttgttacctc
     5701 atgctgtcaa tggaagtcgt actccatcca gagccttggc gaccccgggg ctgaaaacga
     5761 aacaataacc cgcgcacagt gcgaggagcc cgggcgtgaa gcaatcagca ggtaatatta
     5821 atggcaagac cagtaggttc tttaaacaaa cgaacaatat cagcaaatac gacagttcca
     5881 ggagaggtaa aaagtttttt acttgcacaa ttcttaaaaa aatggagacg aacaaaaatg
     5941 gggcgacata cctggaaagt atcgctacca gatgatgcta aaaagcagaa atagtgttcc
     6001 aggcctgaaa gggatgagaa tcagtatctg tgtgtattta taacatcttc ttgaagaggc
     6061 tggagattgt tagtccatta gtataatcga tattcgtttt cctgcgaata tgcaggctac
     6121 accagatata tgattgctac cgaatttcat cagtttgatg aaattacgca tcatactgcg
     6181 ggttttctca ttaacattaa cgaaagtgaa tggactcaga caagtagtga ccggcgcaat
     6241 ttcgctaagt atcaaataca aagaacgttc tcttactttc tgcgatggcc tttccttcgg
     6301 aaaggaagtg acgattaagt gtcgaaaaat gtgatacata tattattttt atggcaaatc
     6361 acctgatagg atttgccata aatgaatgaa aagctgagtg ttaataaatg agtaagaaga
     6421 aaatttctgt tgaaaatgaa gagttcatta cttaccgggc attacttctg agtattgatt
     6481 ttgttaaaaa actggtcaca gaaatgtctg atgaagattt aaataaggtt aaggaaaact
     6541 ttgacatagc tattcagcaa cgtgatgagc gaaaggaaaa tatgaaagct atcaaatcac
     6601 tcatggatga acatggtata tctctggaag atttttatcg tgaatttggc aaaaaatcac
     6661 aggcaggcag aaaaagtgtg tcaggagggc ataaatacga gtatgacgat aacggtaaaa
     6721 gaatgtactg gaatgggcaa ggacgtcggc ctaaggccat agcagaggct ataaaagccg
     6781 gaggttctct tgatgatttt cttatatctc aggaagtatc atctggtcct gattatgaat
     6841 aacagcaata tatattttaa tctgtattaa tatcaaggaa tatatagtaa acacttctcc
     6901 ggagttgata tattatcggc agggaatgaa aatgcctgac t
//
LOCUS       DAPGRN010000184         6918 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPGRN010000184 DAPGRN010000000
VERSION     DAPGRN010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.186"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            457..1446
                     /gene="araF"
                     /locus_tag="RHW33_004410"
     CDS             457..1446
                     /gene="araF"
                     /locus_tag="RHW33_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV1647843.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1516..3030
                     /gene="araG"
                     /locus_tag="RHW33_004411"
     CDS             1516..3030
                     /gene="araG"
                     /locus_tag="RHW33_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV1647844.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3045..4031
                     /gene="araH"
                     /locus_tag="RHW33_004412"
     CDS             3045..4031
                     /gene="araH"
                     /locus_tag="RHW33_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV1647845.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4198..4998
                     /gene="otsB"
                     /locus_tag="RHW33_004413"
     CDS             4198..4998
                     /gene="otsB"
                     /locus_tag="RHW33_004413"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV1647846.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDINVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALLQKRENNRSDDYESFSRSI"
     gene            4973..6397
                     /gene="otsA"
                     /locus_tag="RHW33_004414"
     CDS             4973..6397
                     /gene="otsA"
                     /locus_tag="RHW33_004414"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV1647847.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6404..6832)
                     /gene="uspC"
                     /locus_tag="RHW33_004415"
     CDS             complement(6404..6832)
                     /gene="uspC"
                     /locus_tag="RHW33_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV1647848.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKYH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1740 a         1630 c         1805 g         1743 t
ORIGIN      
        1 atacgtgttc tgttaattaa tgagcacggt gataattaat ggcgaaatga atgacgcgca
       61 tttcccacat ctttgagttg cggttattac gccatttcaa aagctcaacg tcaggtaatg
      121 cggcctattg actggttaaa aagaagacat cccgcatggg taccaaagac aacaaggatt
      181 tccaggctaa tcttatggat taatctgctg tgcattcgac aatttgtctg acaaattggc
      241 tttcccttat gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg
      301 atattgctct cctatggaga attaatttct cgctaaaact atgtcaacac agtcacttat
      361 cttttagtta aaaggtaatg ctttgttttc cgattaattt aacgaatgtc attcgttttt
      421 gccctacaca aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg
      481 gcagccattg gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc
      541 ggtttcctgg tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat
      601 aaagccggga aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa
      661 acattgaacg cgatcgatag cctggccgcc agtggcgcaa aaggtttcgt tatttgtact
      721 ccggacccga aactcggctc tgccatcgtg gcgaaagcgc gtggctacga tatgaaagtc
      781 attgccgtgg atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttcctctg
      841 gtgatgatgg cggcgaccaa aattggcgaa cgtcagggcc aggaactgta taaagagatg
      901 cagaaacggg gttgggatgt caaagaaagc gcggtgatgg cgattaccgc caacgaactg
      961 gataccgccc gccgccgtac aacgggttct atggacgcac tgaaagcggc cggattcccg
     1021 gaaaaacaaa tttatcaggt acctaccaaa tctaacgaca tcccgggggc atttgacgct
     1081 gccaactcaa tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac
     1141 gacagcaccg tacttggcgg cgtacgtgca accgaaggtc agggctttaa agcagccgat
     1201 atcatcggta ttggtattaa cggtgtggat gccgtaagcg aactgtctaa agcacaggca
     1261 accggcttct acggttccct gctgccaagc ccggatgttc atggctataa atccagcgaa
     1321 atgctttaca actgggtagc aaaagacgtt gaaccgccaa aatttaccga agttaccgac
     1381 gtggtactga tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt
     1441 aagtaatttg ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg
     1501 attcacggag acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa
     1561 acgtttcccg gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt
     1621 catgcgttga tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc
     1681 aactatgcgc caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac
     1741 acgaccgcag cacttaacgc gggtgtggcg attatttacc aggaactgca tctcgtgccg
     1801 gaaatgaccg tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggcattgtg
     1861 aatcgctcat tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt
     1921 gacccggaca cgccgctgaa atatctctcc attggtcagt ggcagatggt tgaaatcgcc
     1981 aaggcgctgg cgcgtaacgc taaaattatc gcctttgatg agccaaccag ctccctttct
     2041 gcccgcgaaa tcgacaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg
     2101 atcttatacg tttctcaccg tatggaagaa atatttgccc tcagcgatgc catcactgtc
     2161 tttaaagatg gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg
     2221 ctggtgcagg cgatggtcgg gcgcgacatt ggcgatatct acggctggca accgcgtagt
     2281 tatggcgagg agcgcctgcg tcttgatgct gtgaaagcac caggcgtgcg tacgccaata
     2341 agtctggcgg ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg ggcggggcgt
     2401 agcgaattaa tgaaaggcat gtttggcggg acgcaaatca ccgccggtca ggtttatatc
     2461 gaccaacagc cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgctc
     2521 tgcccggaag atcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc
     2581 aacatcagtg ccagacgtaa acatgtgctc ggcggttgtg taatcaacaa cggttgggaa
     2641 gaaaacaatg ccgatcacca cattcgttcg ctcaacatca aaacgccggg cgcggagcaa
     2701 ctgatcatga atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg
     2761 gaagagatga aggtcatttt gctggatgaa cctacgcgcg gcattgatgt tggcgctaag
     2821 cacgaaatat ataacgtaat ttatgcgctg gcggcgcagg gcgtggcggt gctgtttgcc
     2881 tccagcgact tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggt
     2941 gaaatcgccg gtgaattgtt gcacgagcag gcagatgagc gtcaggcact gagccttgcg
     3001 atgcctaaag tcagccaggc tgttgcctga gtaaggagag tatgatgtct tctgtttcta
     3061 catcggggtc tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg
     3121 gcatgctggt ggtgtttgcg gtgctcttta tcgcctgtgc catttttgtc ccgaactttg
     3181 ccaccttcat taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggcgt
     3241 gtggcatgtt gttctgcctt gcttccggtg actttgacct ttctgtcgcc tccgtaattg
     3301 cctgtgcggg tgtcaccacg gcggtggtta tcaacctgac tgaaagcctg tggattggcg
     3361 tggcagcggg gttgctgctt ggcattctct gtggcctggt caatggcttt gttatcgcca
     3421 aactgaaaat aaatgctctg atcacgacac tggcaacgat gcagattgtt cgaggtctgg
     3481 cgtacatcat ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg
     3541 gttacgctaa ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta
     3601 tctttggttt gctgctgaat aaaaccacct ttggtcgtaa taccctggcg attggcggga
     3661 acgaagaggc tgcgcgtctg gcgggtgtac cggttgttcg caccaaaatt attatctttg
     3721 ttctctctgg cctggtatct gcgatagccg gaattattct ggcttcacgt atgactagtg
     3781 ggcagccaat gacgtcgatt ggttatgagc ttattgttat ctccgcctgc gttttaggtg
     3841 gcgtttctct gaaaggtggc atcggaaaaa tctcatatgt ggtggcgggt atcttaattt
     3901 taggcaccgt ggaaaacgcc atgaacctgc ttaatatttc tcctttcgcg cagtacgtgg
     3961 ttcgcggctt aatcctgctg gcagcggtga tcttcgaccg ttacaagcaa aaagcgaaac
     4021 gcactgtctg atgctttttt ccacaacaat ttaacgtttt ttcccaccac agccagccgc
     4081 cacaacggtt ggctgttctt cgttgcaaat ggcgaccccc gtcacactgt ctatacttac
     4141 atgtctgtaa agcgcgttct gcgcaaaaca ataagaaaag agaaggagga gaaccgggtg
     4201 acagaaccgt taaccgaaac ccctgaacta tccgcgaaat atgcctggtt ttttgatctc
     4261 gatggaacgc tggcggaaat caaaccgcat cccgatcagg tcgtcgtacc tgacaatatt
     4321 ctgcaaggac ttcagctact ggcaaccgca agtgatggtg cattggcatt gatatcaggg
     4381 cgctcaatgg tggagctgga cgcactggca aaaccttatc gcttcccgtt agcgggcgtg
     4441 catggggcgg agcgccgtga catcaatggt aaaacgcata tcgttcatct gccggatgcg
     4501 attgcgcgtg atattaacgt gcaactgcat acggtcatcg cacagtatcc cggtgcggag
     4561 ctggagacga aagggatggc ctttgcgctg cattatcgtc aggctccgca gcatgaagac
     4621 gcattaatga cattagcgca acgtattact cagatctggc cacaaatggc gttacagcag
     4681 ggaaagtgtg ttgtcgagat caaaccgaga ggtaccagta aaggtgaggc gattgctgcc
     4741 tttatgcagg aagctccctt tatcgggcga acgcctgtat ttctgggcga tgatttaacc
     4801 gatgaatctg gcttcgcagt cgttaaccgg ctgggcggaa tgtcagtaaa aattggcaca
     4861 ggcgcaactc aggcatcatg gcgactggcg ggcgtaccgg atgtctggag ctggcttgaa
     4921 atgataacca ccgcattact acaaaaaaga gaaaataaca ggagtgatga ctatgagtcg
     4981 tttagtcgta gtatctaacc ggattgcacc accagatgag cacgccgcca gtgccggtgg
     5041 ccttgccgtt ggcatactgg gggcactgaa agccgcaggc ggactgtggt ttggctggag
     5101 tggtgaaaca gggaatgagg atcagccgct aaaaaaggtg aaaaagggta acattacgtg
     5161 ggcctctttt aacctcagcg aacaggacct tgacgaatac tacaaccaat tctccaatgc
     5221 cgttctctgg cctgcttttc attatcgcct cgatctggtg caatttcagc gtcctgcctg
     5281 ggacggctat ctacgcgtaa atgcgttgct ggcagataaa ttactgccgc tgttgcaaga
     5341 cgatgacatt atctggatcc acgattatca cctgttgcca tttgcgcatg aattacgcaa
     5401 acggggagtg aataatcgca ttggtttctt tctgcatatt cctttcccga caccggaaat
     5461 cttcaacgcg ctgccgacat atgacacctt gcttgaacag ctttgtgatt atgatttgct
     5521 gggtttccag acagaaaacg atcgtctggc gttcctggat tgcctttcta acctgacccg
     5581 cgtcacgaca cgtagcgcaa aaagccatac agcctggggc aaagcgtttc gaacagaagt
     5641 ttacccgatt ggcattgagc caaaagaaat agccaaacag gctgccgggc cactgccgcc
     5701 aaaactggcg caacttaaag cggaactgaa aaacgtacaa aatatctttt ctgtcgaacg
     5761 gctggattat tccaaaggtt tgccagagcg ttttctcgcc tatgaagcgt tgctggaaaa
     5821 atatccgcag catcatggta aaattcgtta tacccagatt gcaccaacgt cgcgtggtga
     5881 tgtgcaagcc tatcaggata ttcgtcatca gctcgaaaat gaagctggac gaattaatgg
     5941 taaatacggg caattaggct ggacgccgct ttattatttg aatcagcatt ttgatcgtaa
     6001 attactgatg aaaatattcc gctactctga cgtgggctta gtgacgccac tgcgtgacgg
     6061 gatgaacctg gtggcaaaag agtatgttgc cgctcaggac ccagccaatc cgggcgttct
     6121 tgttctttcg caatttgcgg gagcggcaaa cgagttaacg tcggcgttaa ttgttaaccc
     6181 ctacgatcgt gacgaagttg cagctgcgct ggatcgtgca ttgactatgt cgctggcgga
     6241 acgtatttcc cgtcatgcag aaatgctgga cgttatcgtg aaaaacgata ttaaccactg
     6301 gcaggagtgc ttcattagcg acctaaagca gatagttcca cgaagcgcgg aaagccagca
     6361 gcgcgataaa gttgctacct ttcctaagct tgcgtaggag cggttaatct cccgtaagtg
     6421 gaactaacag cacatcgacc tcacttgagg caatcactct tttcgccgag caggatgctc
     6481 gcgaaaagaa actgtgattg tgattaccgc aaatcaccaa atcgaaatga tatttgcgac
     6541 aaacttccag aatatgttcg cttaactcac cgtaggcaat aaacgtcttg tcaacgggat
     6601 accccgcatc ctgaattaac ttatcaagaa agctttgcgt ttcttcctgc atcacgctgc
     6661 gtaaatcttc aagcatcggc gcagctaatt gattgtacat ttccgggtcg gaagcgagag
     6721 taattaaact gatgtgtccc tttactggcc tggcgataga taccgctttt gccagcagtt
     6781 gctgactttc cggtgtaacc gcaacagcga caagaatatt gctatagctc ataacctgtt
     6841 ccttattctg tgaacttcag gtgacattaa agctttactc ctaatggaga aacgacgcaa
     6901 tcccaactca gtcaaaca
//
LOCUS       DAPGRN010000185         6791 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPGRN010000185 DAPGRN010000000
VERSION     DAPGRN010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.187"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(291..1631)
                     /gene="fadL"
                     /locus_tag="RHW33_004416"
     CDS             complement(291..1631)
                     /gene="fadL"
                     /locus_tag="RHW33_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV1647849.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVDISGRSDLTGQRADADNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFNDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERTAGSLGPLLSQQLIAQGVPQSWTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFEGDYKSSINPNLNNILGSMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSLAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYHDKNWTFRTGIAFDDSPVPADKRSISIPDQDRLWLSA
                     GATYAFNEDASIDVGASYMHGQNVKFTEGEGPAAYTFKSEGKAWLFGTNFNYAF"
     gene            2003..2287
                     /locus_tag="RHW33_004417"
     CDS             2003..2287
                     /locus_tag="RHW33_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV1647850.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2468..3778
                     /gene="fadI"
                     /locus_tag="RHW33_004418"
     CDS             2468..3778
                     /gene="fadI"
                     /locus_tag="RHW33_004418"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV1647851.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQLLAEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     ADLTLIDMHEAFAAQTLANIQLLGSERFARNVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3778..5922
                     /gene="fadJ"
                     /locus_tag="RHW33_004419"
     CDS             3778..5922
                     /gene="fadJ"
                     /locus_tag="RHW33_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV1647852.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLDGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMLLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGVQGQGRLFAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6125..6610
                     /gene="sixA"
                     /locus_tag="RHW33_004420"
     CDS             6125..6610
                     /gene="sixA"
                     /locus_tag="RHW33_004420"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV1647853.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         1670 a         1596 c         1800 g         1725 t
ORIGIN      
        1 ttatttagaa cctatcttta atttgagtgt atgagcgcac aatagccagg gggttgagta
       61 tcctggaaat tcagaaaagt aaactctttg agaaggacga gcagaacgca tgaaaatatg
      121 cgtttccctt caggtttgtc tgtggatacc acttactgat ttgaaagggc aagctgccat
      181 caggctgttt gcaaaactca ggaggcaagc aatgaggaaa ctactttcgg tgaagtgggg
      241 gagttaaagt cacctgcata gcaggtgact ttatccaggc gaacgcgtta tcagaacgcg
      301 tagttaaagt tagtaccgaa cagccaggct ttaccttcag atttaaaggt atatgctgct
      361 ggcccttcac cttcagtaaa cttcacattc tgaccgtgca tataagatgc accaacgtca
      421 atggatgcat cttcattaaa tgcataagtc gcacctgcac ttaaccacag acgatcctga
      481 tctgggatag agatagaacg tttatctgcc ggaaccgggc tgtcatcgaa tgcaataccc
      541 gtacggaagg tccagttttt atcgtggtag taggttgtac ctaacgcgat acggtaagca
      601 tcccggaaac cttcgtcttt atagaacagc gtttgaccag agtctccttt cgctttcagt
      661 tcctggaatt gactccaact ggtatacgcc agactatagt ggatagccca ttgtggtgcg
      721 acacggttat agccagaaat ttcccacatc tcaggtaaat tcagcgttaa ggacccattt
      781 tgggttgcac ccattgtgcc atatggtaaa cccatgctac ccaaaatgtt gttcagattt
      841 gggttgatgg agcttttgta atctccttca aagtcaattt tcacttctga acgataggtt
      901 aggccccaac ggttatcttt atccagttca taaagaatac cggcgttcca gccgaatccc
      961 cattcatcac ccttgagatg agcaatttgc gaatccggtg tattgatgcc gtttgtccaa
     1021 ctttgtggca cgccttgcgc tatcagttgt tgggaaagca atggaccgag tgaaccggca
     1081 gtacgctcaa tttttgcctt agcgtataca gcatcaaaac caagaccaaa actccagcta
     1141 ttgttaaggc ggtaggctcc gctaaggtta aggttgatcg tctgcagatc ggttttaccg
     1201 ccatagattc cagcagcgta gttatcatta aattccgtgg ccagaccata gttcgaggta
     1261 atagaagccc cccaaccaaa ctgatcgtta ataggtgcta cgaaatgtag gttaggcacc
     1321 cacgcagttg gtgcaatatt atctgcatca gcacgctggc ccgttaaatc ggaacgccca
     1381 gaaatatcta catccggatc aatataaacc gcacccgcag aaaatgtcgg gcggtcaaac
     1441 atggtaatca atgcggggtt acggctgacg ttacctgcat catcggcaat tgcgccttcc
     1501 cctgaataag cccggcccag gccagaggaa gaaaattcgt ttaactgaaa gcctgccgac
     1561 caggcctggg tggagataag tgccactgcg actgcgagag cagactttgt aaacagggtt
     1621 ttctggctca tgaccataac ctcaatgatt tatttttata caaactatgt tacgtgctgt
     1681 aacaggagcg cgaagtgtag ggtctgaggt acatcttaga aatcagacca gtggcgagag
     1741 tataggtcgg accagctgga atgttgcaag tatgtttcta tcttttttca aatatgatct
     1801 aagaaacgcg atccagctgg caaaatttgg aggtggcgca ctcatggatt cgggtgaatt
     1861 ttgttttaga tcatttttaa gtgtgatttc ggtcacttat ccgattttgc agaattaaca
     1921 actggagcag cactttccgg aagcgtaaaa tatccacaac gtttatctgg caccttgggt
     1981 gcacatacag aggaaatcaa cgatgagtaa atgtagtgct gatgaaaccc cggtttgctg
     2041 ctgtatggat gttggcacca ttatggacaa ctccgactgc accgcgtctt acagccgcgt
     2101 gtttgcaaac cgcgcagaag cagaacaaac gctggcggcg ctgactgaga aagcccgtag
     2161 cgtggaatcc gaaccgtgca aaatctcccc gactttcact gaagagtctg atggtgtgcg
     2221 cctggatatc gatttcactt ttgcctgcga agctgaaatg ctgatcttcc agctgggtct
     2281 gcgttaattc ctctcgggta cgtacgcccc tgtcagttgt tggcaggggc gttttctttt
     2341 ctcttctact tttctctctg tgtcatagct cccactttcc cttttctcca cttggctaaa
     2401 tgtaaaaaaa tggttaagac tatgatcagg tcagaccact ttatttatta ttttacaggg
     2461 gagtgttatg ggtcaggttt taccgctggt tacccgccag ggcgatcgta tcgccattgt
     2521 tagcggttta cgtacgcctt ttgcccgcca ggcgacggct tttcatggca ttcccgcggt
     2581 tgatttaggg aagatggtgg taggcgaact gctggcacgc agcgagatcc ccgccgaagt
     2641 gattgaacaa ctggtctttg gtcaggtcgt acaaatgcct gaagctccca acattgcgcg
     2701 tgaaattgtt ctcggtactg gaatgaatgt gcataccgat gcttacagcg tcagccgcgc
     2761 ttgcgctacc agtttccagg cggttgcaaa cgtcgcagaa agcctgatgg cggggactat
     2821 ccgagcgggg attgccggtg gggcagattc ctcttcggta ttgccaattg gcgtcagtaa
     2881 aaaactggcg cgcgtgctgg ttgatgtcaa caaagcccgc acaatgagcc agcgtctgaa
     2941 actcttctct cgcctgcgtt tgcgcgactt aatgcccgta ccacctgcgg tagcagaata
     3001 ttctaccggc ttgcggatgg gcgacactgc agagcaaatg gcgaaaacct acggcatcac
     3061 ccgagaacag caagatgcat tagcgcaccg ttcgcatcag cgtgccgctc aggcatggtc
     3121 agacggaaaa ctcaaagaag aggtgatgac tgcctttatc cctccttata aacaactgct
     3181 cgcggaagac aacaatattc gcggtaattc ctcgcttgct gattacgcaa agctgcgtcc
     3241 ggcgtttgat cgtaaacacg gaacggtaac ggcggcgaac agtacgccgc tgaccgatgg
     3301 cgcagcggcg gtgatcctga tgactgaatc ccgggcgaaa gaattagggc tggtaccgct
     3361 gggatatctg cgcagctacg catttaccgc tatcgatgtc tggcaggaca tgttgctcgg
     3421 tccagcctgg tcaacaccgc tggcgctgga gcgtgccggt ttgacaatgg cagatctgac
     3481 attgattgat atgcacgaag cctttgccgc ccagacgttg gccaatattc agctactggg
     3541 gagcgaacgt tttgctcgca acgtactggg gcgtgcgcat gccaccggtg aagtggacga
     3601 tagcaaattt aacgtgcttg gtggctcgat tgcatacggg catccctttg cggcgaccgg
     3661 cgcgcgaatg ataacccaga cattgcatga acttcgccgc cgcggcggtg gatttggttt
     3721 agtgaccgca tgtgctgccg gtgggcttgg cgcggcaatg gttctggagg cggaataatg
     3781 gaaatggcat cagcgtttac tcttaatgtt cgtctggaca acattgctat catcaccatc
     3841 gacgtaccgg gtgagaaaat gaataccctg aaggcggagt ttgcctcgca ggtgcgcgcc
     3901 attattaagc aactccgcga aaacaaagag ttgcgaggcg tggtgtttgt ctccgctaaa
     3961 ccggacaact tcattgctgg cgcagacatc aacatgatcg gcaactgcaa aacggcgcaa
     4021 gaagcggaag ctctggcgcg gcagggccaa cagttgatgg cggagattca tgctttgccc
     4081 attccggtta tcgcggctat tcatggcgct tgcctgggtg gtgggctgga gttggcgctg
     4141 gcgtgccacg gtcgcgtttg tactgacgat cctaaaacgg tgctcggttt gcctgaagta
     4201 caacttggat tgttacccgg ttcaggcggc acccagcgtt taccgcgtct gataggcgtc
     4261 agcacagcat tagagatgat cctcaccgga aaacaacttc gggcgaaaca ggcattaaag
     4321 ctggggctgg tggatgacgt tgttccgcac tccattctgc tggaagccgc tgttgagctg
     4381 gcaaagaagg atcgcccatc ttcccgccct ctacctgtac gcgagcgtat tctggcgggg
     4441 ccgttaggtc gtgcgctgct gttcaaaatg gtcggcaaga aaacagaaca caaaactcaa
     4501 ggcaattatc cggcgacaga acgcatcctg gaggttgttg aaacgggatt agcgcagggc
     4561 accagcagtg gctatgacgc tgaagcacgg gcgtttggcg aactggcgat gacgccacaa
     4621 tcgcaagctt tgcgtagtat cttttttgcc agtacggaag tgaagaaaga tcccggtagt
     4681 gatgcgccgc ctgcgccatt aaacagcgtg ggaattttag gtggtggctt gatgggcggc
     4741 ggtattgctt atgtcactgc ttgtaaagcg gggcttccgg tcagaattaa agatatcaac
     4801 ccgcagggca taaatcatgc actgaagtac agttgggatc agcttgatgg caaagtgcgc
     4861 cgtcgccatc tcaaagccag cgaacgtgac aaacagctgg cattaatctc cggaacgacg
     4921 gactatcgcg gctttgccca tcgcgatctg attattgaag cggtgtttga aaatctcgaa
     4981 ttgaaacaac agatggtggc ggaagttgag caaaattgcg ccgctcatac catctttgct
     5041 tcgaatacgt catctttacc gattggtgat atcgccgctc acgccacgcg acctgagcaa
     5101 gttatcggcc tgcatttctt cagtccggtg gaaaaaatgc tgctggtgga gattattcct
     5161 catgcgggta catcagcgca aaccatcgcc actacggtaa aactggcgaa aaaacagggc
     5221 aaaacgccca ttgtcgtgcg tgacaaagcc gggttttacg tcaatcgcat cttagcgcct
     5281 tacattaatg aagctatccg catgctgacc gaaggtgaac gggtagagca cattgatgcc
     5341 gcgctagtga aatttggttt tccggtaggt ccaatccaac ttttggatga ggtaggaatc
     5401 gacaccggga ctaaaattat tcctgtactg gaagccgctt atggagaacg ttttagcgcg
     5461 cctgcaaatg ttgtttcttc aattttgaac gacgatcgca aaggcagaaa aaatggtcga
     5521 ggtttctatc tttatggtca gaaagggcgt aaaagcaaaa aacaggtcga tcctgccatt
     5581 tacccgctca ttggcgtaca agggcagggg cgactcttcg caccgcaggt tgctgaacgg
     5641 tgtgtgatgt tgatgctgaa tgaagcagta cgttgtgttg atgagcaggt tatccgtagc
     5701 gtgcgtgacg gggatattgg tgcggtattt ggcattggtt ttccgccatt tctcggtgga
     5761 ccgtttcgct atatcgattc tctcggcgcg ggcgaagtgg ttgcaataat gcaacgactg
     5821 gccacgcagt atggttcacg ttttacccct tgcgagcgtt tagtcgagat gggtgcgcgt
     5881 ggggaaagtt tttggaaaac aactgcaact gacctgcaat aagaaggtca aagctatatg
     5941 aatccgcgct gaatggcgga ttgttggtca aaatgtaaac gcatattgac tatacttacg
     6001 ccattgaggt aaaaaacagc gtttcattcg gtgaatggat aaggcacaat gccggccacc
     6061 gttttctttc tctggtttca gatgaaagaa aacgggcgaa tctggttaac aaaagcggtg
     6121 caatatgcaa gtttttatca tgcgtcacgg cgacgcagcc ctcgatgccg ccagtgattc
     6181 ggttcgtcct ctgactacta atggttgtga cgaatctcgc ctgatggcga actggctgaa
     6241 aggtcaaaaa gtggaaatcg aacgcgttct ggtgagcccg ttcctgcgag ccgagcaaac
     6301 gctggaggaa gtaggggatt gtctgaacct gccttccagt gcagaagttc tgccggagtt
     6361 aacgccctgc ggcgatgttg gtctggtcag tgcctatttg caggcgttga ccaatgaagg
     6421 tgttgcttcg gtgttggtta tctcccactt accgttagtt gggtatctgg tcgccgagtt
     6481 atgtccgggc gaaacgccgc ctatgtttac cacctcggcc attgccagcg tcacgcttga
     6541 tgaaagcgga aacggcacat tcaactggca gatgagcccg tgtaatttga agatggcgaa
     6601 agctatctga tttgtttgaa aaaatgcccg atgtaagctg tacatcgggc atttgtcttt
     6661 ttgcaatagt ataaattcac tcaaaaccct tcacggagtt ggtatgctgc gatgatgatt
     6721 tcatcgcact ctttcctctg cttccctatt aatttctgat gaaatgagac taatttgtaa
     6781 tatattttta c
//
LOCUS       DAPGRN010000186         6673 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPGRN010000186 DAPGRN010000000
VERSION     DAPGRN010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.188"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(209..484)
                     /locus_tag="RHW33_004421"
     CDS             complement(209..484)
                     /locus_tag="RHW33_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647854.1"
                     /translation="MTPEKHPETTMKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMP
                     QETKRRLRARILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            718..2811
                     /locus_tag="RHW33_004422"
     CDS             718..2811
                     /locus_tag="RHW33_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HDV1647855.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNEPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2808..3071
                     /locus_tag="RHW33_004423"
     CDS             2808..3071
                     /locus_tag="RHW33_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin D immunity protein"
                     /protein_id="HDV1647856.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3141..3287
                     /locus_tag="RHW33_004424"
     CDS             3141..3287
                     /locus_tag="RHW33_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HDV1647857.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            complement(4332..4523)
                     /locus_tag="RHW33_004425"
     CDS             complement(4332..4523)
                     /locus_tag="RHW33_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HDV1647858.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4591..4914
                     /locus_tag="RHW33_004426"
     CDS             4591..4914
                     /locus_tag="RHW33_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDV1647859.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            4877..5620
                     /locus_tag="RHW33_004427"
                     /pseudo
     CDS             4877..5620
                     /locus_tag="RHW33_004427"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5592..6152
                     /locus_tag="RHW33_004428"
     CDS             5592..6152
                     /locus_tag="RHW33_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HDV1647860.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6159..6392
                     /locus_tag="RHW33_004429"
     CDS             6159..6392
                     /locus_tag="RHW33_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HDV1647861.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
BASE COUNT         1666 a         1416 c         1933 g         1658 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 taaggggtta tgttaaataa cccttagctt agcggcggcg cagtggttgc caggtgaagg
      241 tgtcatccgg gtcctgtccg gcagtcagtg tggcttctcg atttttgctg gccgttcgtt
      301 catcaagaat ggctttaagt atccgtgccc ggaggcgtcg ctttgtttcc tgcggcattt
      361 tgtccttttt cgtcagcccc atggcacgag cttcagcggc aatggcttct gccagtcggc
      421 gctcttcggc ttcggtcagt ctatggcgtt tcatggtggt ctccgggtgt ttctctggtg
      481 tcataacagt ttaactgttg gctctctcct tatgctaatc agccttcttt catgcctgtt
      541 gccattgagt ttaagaatga ttggttgttt ttcttgagcg ttttcacaaa aacgtaaatc
      601 cagtgggttg acaggattta cgaggtgacg taacttttta ctgtacataa aaccagtggt
      661 tttatgtaca gtttgttgtt tttaacgcat tgttttaaaa gtcaaagagg tgtttttatg
      721 agtgattacg aaggtagtgg tccgacagaa ggtattgatt atgggcactc gatggtcgtg
      781 tggccgtcaa caggactgat ttcgggtggt gatgtgaaac caggaggctc atcaggtatc
      841 gctccatcca tgcctccggg atggggggat tacagcccac agggtatcgc acttgtacaa
      901 agtgttcttt ttccgggaat tattcgccgg attattctgg ataaggaact tgaagaggga
      961 gactggtcgg gatggtctgt cagtgtgcat agcccttggg gaaacgagaa agtttccgct
     1021 gcacgaacag ttcttgagaa tggtttacgt ggtggtttgc cagaaccgtc tcgcccggct
     1081 gctgtttctt ttgcccgtct ggagcctgct tccggaaatg agcaaaaaat tattcgtctt
     1141 atggttacac agcaactgga acaggtaacg gatattccgg ccagccagtt accagcagcg
     1201 ggtaataatg taccggtgaa atatcgtctg atggacctta tgcagaacgg tacgcaatat
     1261 atggctatta tcggaggtat tccgatgaca gtgccggtag ttgatgccgt tccagttccg
     1321 gaccggagtc gtccgggaac aaatattaaa gatgtttaca gtgcccctgt atcaccaaat
     1381 ctaccggacc tggtattaag tgtgggtcag atgaatactc cggttctgtc taatcccgaa
     1441 attcaggaag aaggagttat tgctgagaca ggtaattatg ttgaggctgg ttatacgatg
     1501 tccagtaata atcatgatgt cattgttcgt tttcctgaag gcagtggtgt ttctccgtta
     1561 tatatttcaa ctgtagagat tctggacagt aatggtctga gtcagcgtca ggaagccgaa
     1621 aataaagcaa aggatgattt cagagtcaag aaagaagaag cggtagcccg ggctgaagct
     1681 gaaaaagcaa aagccgagtt atttagtaaa gcaggtgtga accagcctcc tgtatataca
     1741 caggaaatga tggaaagggc taattcagta atgaatgagc agggagctct tgttctgaat
     1801 aacactgcca gttctgtaca actggcgatg accggaactg gtgtctggac tgctgctggt
     1861 gatattgcag gcaacataag caagtttttc agtaatgctc tggagaaagt aaccataccc
     1921 gaagtgagtc ccctgcttat gcggatttct cttggtgctc tgtggtttca ttcagaagaa
     1981 gctggagcag gaagtgatat cgtgccgggg cgaaatctgg aggcgatgtt ttcgctgagt
     2041 gctcagatgt tggctggaca gggcgtggtc attgaacctg gtgcgacgag tgtaaatctg
     2101 cctgttcgtg gacaattgat aaacagtaac gggcaattag ctctggattt actgaaaaca
     2161 gggaatgaaa gtatcccagc tgcggttcct gttcttaatg ctgtccgtga tacagcaaca
     2221 ggactggata aaatcacgtt accagcagta gtaggcgcac cttcccggac gattctggtt
     2281 aatccggtac cacaaccttc ggtgccaaca gatacaggta atcatcaacc cgttccggtt
     2341 acaccagtgc atacaggaac ggaagtaaaa ccggtcgaaa tgccagtaac gacgattact
     2401 cctgtttctg atgttggtgg actacgagat tttatttact ggcgtcctga tgctgctggg
     2461 actggtgttg aagctgttta tgtgatgctt aatgaacctc tggattcagg gcgattttcg
     2521 cgtaaacaac ttgataaaaa atataaacat gctggtgatt ttggtattag tgatacaaaa
     2581 aagaatcgtg aaactcttac taaattcagg gatgctattg aggagcattt atcggataag
     2641 gatacagtag agaaaggaac ataccgaaga gaaaaaggtt caaaagttta ttttaatcct
     2701 aatacgatga atgtggttat aattaagtca aatggtgagt tcttatctgg gtggaaaata
     2761 aatccagatg cggataatgg tcgaatttat ttagagacag gtgaactatg aacaagatgg
     2821 caatgatcga tttggcgaaa ttatttttag cttcgaaaat tacagcaatt gagttttcag
     2881 agcgaatttg tgttgaacgg agaagattgt atggtgttaa ggatttgtct ccgaatatat
     2941 taaattgtgg ggaagagttg tttatggctg ctgagcgatt tgagcctgat gcagataggg
     3001 ctgattatga aattgatgat aatggactta aggttgaggt ccgatctatc ttggaaaaat
     3061 ttaaattata atcatgagtg attttaatat ctggcattta agtataaatg tcagatattc
     3121 actcatgaca aggagatgtc atgaaaaaaa agataggggg atctatgatt ctcgttcttg
     3181 ctgttttgtg tctgactgct tgccaagcta actatgtaag agatgttcag ggtggaacaa
     3241 ttgctccatc atcatcttcg aaactaatcg gggtggcggt tcagtgaaaa atattaaagg
     3301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaacccgc
     3361 cttgtggcgg gtccttccgt ccgatcagaa gtcttcgacc tcttctaatc ggcggtggta
     3421 actggcttgg aggagcacag tcaccaaaac tgtcttttca gtttagcctt aactggttca
     3481 taacttcaag agcaactcct ctaaatcagt taccaatggc tgctgccagt ggcgttaagt
     3541 cgtgtctttt ccgggttggc cccaagagaa cagttaccgg agaaggtgca gcagtcgggc
     3601 tgaacggggg gttcctgcat acagcccagc ccggagcgaa ctgcctaagt cgccccgaga
     3661 taccagaaga cgtgagctat gagaaagcgc cacgtgtccc gcaagggaga aaggcggaaa
     3721 aggtatccgg taatcgatat ggcgggaacg tgggggcgca ggagggagcg attcaccgga
     3781 aacggtgatg atctttaagc cacgtcgggt ttcgccaccg ctggcggatt tcatggttga
     3841 gcctcatggc tcccacagat gcacagaaaa agcgtctgtt ttgtgaagtc cgtcaggggg
     3901 gcggagccta tggaaaaacg ccagcggcgc gacttttttt gccctttgcc tttttgctca
     3961 catgttcttt ccggccttat cccctgattc tgtggataac actatcaccg cttttgagtg
     4021 ggcagatacc gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     4081 ggaaaagcgc ctggacgagc attttctcct tacgcatctg tgcggcattt cacacccgcc
     4141 atggcgcact tttcatacaa ttctcactga tgccatatga ttaagccagt atacactccg
     4201 ctatcgctat gtgactgggt catggctgcg ccccgacacc cgctaaaacc cgctgacgcg
     4261 ccctgacggg cttgtctgct cccggcatcc gctcacaggc cagctgtgac cgtctccggg
     4321 agctgcatgt gtcagaggtt ttcaccgtca tccccgaagc gtgcgaggca gctgcggtaa
     4381 agctcgtcag cgtggtcgtg aagcgcttcg cagatgtcag cctggtcgtc ggcgtccagt
     4441 tcattcagtt tttccagcag ggtcagcgtc tggcttttga tgaagcgggc catgttaagg
     4501 gcggtttttt cctgtttagt cacagttctg tttctccatg tgtggggagt ctgtcggagg
     4561 tttttaatga taccggtgaa acgcgaaagg atgctcacga tacgggtcac ggatgaggaa
     4621 catgcgcggc tgctggcacg ttgtgaggga aaacaacttg cgtcatggat gcgtaaggtc
     4681 tgcctggggg caccgccatc gaaaacgtca ggtcttccga cactggcacc gccgctgttg
     4741 cgccagttcg cttcggtcgg caataacctg aatcagatag cccggaagat aaacagcgga
     4801 cagtggtcgg ggcatgaccg ggttcatgtg gtggctgcac tgatggccat cggacgtgaa
     4861 ctgagtgagc tgagggatga ggtccgtaaa cagggagaac gggatgatag ttaagtttca
     4921 tgccagggga aaaggcggtg gcagtggtcc ggttgattac ctgctgggcc gggagcgtag
     4981 ccgggaaggg gcgagagttc tgcggggtgc tcccgaagag gtgcgggaac tcatcgatgc
     5041 cacgccgttt gcgaaaaaat acacctcggg ggttctgtct tttgcagagc agacactgcc
     5101 accgggagag cgtgaaaggg tgatggagag ctttgagcgg gtactgatgc ccggtcttga
     5161 aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac aaggggcgtc ttgagctgaa
     5221 ttttgtcatc ccgaatatgg agctggcgag cggaaaacgg ctccagccct attacgaccg
     5281 ggcggaccgt ccgcgaatca atgcctggca gacactggtg aatcaccatt acgggctgca
     5341 cgacccgaac gcaccggaga acaggcggac acttgtcacc ccgaataatc tgccgaaagc
     5401 gaaacaggag gccgcagaag cgattacgag gggagtggag gctctttacc atgccggagc
     5461 gctaaaaacg cgtcaggatg tcactgaggc gcttacagcg gcaggttttg aggtggtgag
     5521 gacaaccagg agcagtatca gtatcgccga cccggagggc gggcgtaatc tcaggctgaa
     5581 aggggcgctg tatgagcaat cttttacaaa tggggacaga cttcgagaag aaactgaaag
     5641 agcgtgccgt ctctacagag aacatgctga acagcgagtt caggaagctc gaagcgtctg
     5701 tagacaggga actgaactta aacgggcaga aaatcagaga cgctatcagc gcacacacag
     5761 cagcggtgaa agaacaactg gagacgctga acagtgccgt gaacgcgcag ttcagcgcca
     5821 cagagaacga acttgcccgg cggcaggaag agctgctctg gaagctggtc aaagggcgga
     5881 tactgtaccc gtcgctgacg gcgctttgcg tcacggtcgg gatttttctg gcgtcatggg
     5941 ggttgattca gtggcaggaa agccgtattg cggcgaacat cctggctatc agggaccagg
     6001 aagagacgct ggcgaaactc cgggaaaaaa catggggtgt gacgtatcac gagggcagga
     6061 acgggaaatt tctggtgttg ccgtccgggg tgaaggggga aaacaactgg acggtggaga
     6121 agaagaacgc agtaagactg gtcagggagt aagtgagtat gacagagctg gaaatgcact
     6181 tactgaacgc cttagagcaa ttgcagcagg actatacgca gcggctgaaa gactgggaga
     6241 gcgccttcac ggatttgcag catatgttcg agctgacacg acaggagaac gggcaactga
     6301 gggaacagtg cgacggcttg agtcagcagg tgcagagatt agccgggcag gtggaccgct
     6361 tgagtcggtt atttatcacg aacagcagat aagggatgaa caggaaaggc tggcacgcct
     6421 ggaacgggag cgtgcgcggt cctggcatga agagcaggaa cgggaagtac gggtatggcg
     6481 tggcccgtca atggggatgt gacggtgagt tgttccggga ggttgccaga tggtaccgga
     6541 acgcgttaaa aacgaatccg ggcgcgattc tggcgtgtcg tttttgttgc tgtgttttgc
     6601 ctctttgcca gatcgcgctc attcctcagc ctcatcccgc gatgtctgcg tatgtcatca
     6661 actgaaaaga tgg
//
LOCUS       DAPGRN010000187         6664 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPGRN010000187 DAPGRN010000000
VERSION     DAPGRN010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.189"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..320
                     /locus_tag="RHW33_004430"
     CDS             <1..320
                     /locus_tag="RHW33_004430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647862.1"
                     /translation="QIEKLDNGVGKELPSYVNGGVSSIESIRRVHGNVEASATKREAV
                     YKAAIYWLLNDPASCNGKRGKLTIDAWADKIVAERNNPKTKVELGKDKIKECLSSSKA
                     GRF"
     gene            428..637
                     /locus_tag="RHW33_004431"
     CDS             428..637
                     /locus_tag="RHW33_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HDV1647863.1"
                     /translation="MTLKTYPITGHARPRATADFLQVSTVTLWRWEKNNPEFPKSTRI
                     SKRVTVYDAQEIRQWVKAQSSDADK"
     gene            complement(689..868)
                     /locus_tag="RHW33_004432"
     CDS             complement(689..868)
                     /locus_tag="RHW33_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647864.1"
                     /translation="MSTHKNERRGNPPFQFRLDPELRELMEEAQQQDGDESLAAWIKR
                     IIRKELQSRGSEPKN"
     gene            1000..1176
                     /locus_tag="RHW33_004433"
     CDS             1000..1176
                     /locus_tag="RHW33_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV1647865.1"
                     /translation="MAKQKCTWLFAAINRSQRNARPVMLRITADNERSARRRLAPDYV
                     LSFAGRIPCGGEHA"
     gene            1169..1528
                     /locus_tag="RHW33_004434"
     CDS             1169..1528
                     /locus_tag="RHW33_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647866.1"
                     /translation="MRNYPQPGERWQHERGWTVTIIRLTEASPSVALVNPEFSCEVLI
                     RHDSDNKLSSCPLAWFEQRYTRLFNAPLFKPSLAPAGGSGSGSLTLHPSVVFTRWRER
                     SIRRSAEPDDSHYSKFL"
     gene            1560..1844
                     /locus_tag="RHW33_004435"
     CDS             1560..1844
                     /locus_tag="RHW33_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647867.1"
                     /translation="MNISNGHNGAQGHALPEKHNSGAFAYGLSEDGFGKLDRARKACD
                     MLQLLFSEYPSQGGTLDAGCAPGIAALMEYLRADLTDIAHSCELIEGGTQ"
     gene            1841..2224
                     /locus_tag="RHW33_004436"
     CDS             1841..2224
                     /locus_tag="RHW33_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HDV1647868.1"
                     /translation="MNQPQVSIFPAEMTTALYRRAIASAWRQKALTETGCDHYGPHSL
                     TVERIEMAIALHIECALINEYGEAQGAAAALALLTDMLEPSLLTAPPVLTVRGCEVMA
                     ELYRTLPAAFDDFCSSGVSLYQGEI"
     gene            2221..4893
                     /locus_tag="RHW33_004437"
     CDS             2221..4893
                     /locus_tag="RHW33_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDV1647869.1"
                     /translation="MTTQTVTQISAAARGKWPVILQMLRIDVPENGRHGPCPKCGGKD
                     RFRLDDLDGRGTWICSQCGNGDGLDLVKLITGYGIRKAAQEVAQVLNVPDVQELPVKP
                     ARQKAPKRDMSLTVAALMKESHTGESPYLTGKGFAGYPASLTGSVQHISGKDFPAGSL
                     LLPLTTNAGAVTGAQLIAPTGEKSILPGSTMKGAFVALSPLPSEPPVQVVITEGYATA
                     LTVSQLTAGCVVAAISAGNLPNVAQALRARWPEVKIIIAGDNDFQDGGENPGRSFAER
                     AAKSVGGWVTLPPGEIKADWNDFHREHGITRAREAFRNGLVLCGEGRTQLPHGFRLTQ
                     EYLWYEKQVQRNGETEIQNVKICNPLRVTAITCDADGGNFGRLLEWEDTWGERRRWAM
                     PMEMLSGSGEELRRVLLVNGLSYISTTGEARARLMEYISLCKPERRVTCVSRTGWHGQ
                     VYVLQDEVSGEGAEGVILQTTSVQGRDFRVSGTTEEWREHVSRYCTGNSRVAFAVSLA
                     FAAPLLRLVGMDGGGYHLKGESTDGKTTTMKAATSVCGGPDYWQTWRATGNALEGCAS
                     RRNDAAMMLDEIREVDGREAGNIAYMLANGQGKGRAGTDGELRTRKQWRLLFFSTGEL
                     SLTEHAAKAGERTFAGMEVRMIQIPSDSGKFGVFEELHGFDSGKALAEHLEWATSSYY
                     GSPFREWLKALTADLNGLTAQAKSLMKEYTAALTPKDAGNQVGRAVNRFALVAMACEL
                     ATRLGITGWPEGEALRATRVCLNAWLKDRGHTANQEDIAALEQVRSFFTANQYSRFAD
                     WHDERNRPGNMVGWRRVEKGSTAQGTEAVTTFYVMPSGWKEICRGFDPRKVARLCADR
                     GYLLPSTDGKLQTTIRPPEMNPRRLYVFNSEVPG"
     gene            5294..6055
                     /locus_tag="RHW33_004438"
     CDS             5294..6055
                     /locus_tag="RHW33_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HDV1647870.1"
                     /translation="MNNTAENNNLSTFPAVTQQAMETLNTARSAWLEARRRQKAAADN
                     IATIRQRRAEMEATTNALNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFDELL
                     AAQESENALLPQEAGKLAGQYISAHNTLVEIRAKQIWEDFMQAHGKALIQTLSLLKTT
                     MGREASAVVGVVNSVNDPDTLLKDFIHKHITKPALANTAMPELDPVFKLAGVAPDYAA
                     RVDLSKIPSPAALHKMKIRQEREELQKRNRDMEGI"
     gene            6055..6327
                     /locus_tag="RHW33_004439"
     CDS             6055..6327
                     /locus_tag="RHW33_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV1647871.1"
                     /translation="MALKCPECGTVAHARTTAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDKIIMKPRRNEQESDKAQMPEKQQQTLNRYGSASKLSSRQQIPV"
BASE COUNT         1648 a         1708 c         1906 g         1402 t
ORIGIN      
        1 accaaataga aaaattagat aacggtgttg gcaaagagct acccagctat gtgaatggtg
       61 gtgtatccag catcgaaagc atccgaagag tgcatggtaa tgttgaagca tcagcaacaa
      121 aaagagaggc agtgtacaaa gcagctattt attggttact aaatgaccca gcatcttgca
      181 atggaaaacg aggaaaactg actattgacg cgtgggcaga taaaatcgtg gcagaaagaa
      241 acaatccaaa aacaaaggtt gaacttggaa aagataagat aaaagaatgt cttagctcat
      301 ctaaagcagg aagattttaa gtggtaatat tttataccat cataaaaaca aaatagtggc
      361 actgataatt tagccctaac cttgccatca tctgccgtgt tcattacata acacggcagg
      421 taatttcatg acattaaaaa cttaccctat caccgggcat gcccgcccac gggctactgc
      481 tgactttttg caagtaagca cagttactct ctggcgctgg gaaaaaaaca atcctgaatt
      541 tccaaaatcc acacgtattt caaaacgggt aactgtttac gatgcacaag aaattagaca
      601 gtgggtaaaa gctcaatcct cagatgctga taaataatat gacgacctta gaaaaatggg
      661 aaagaagaaa atcttcccca tgtatttatc agttctttgg ttcggaacca cgactttgta
      721 gctctttgcg gataatgcgt ttgatccaag cagccagtga ttcatcacca tcctgctgct
      781 gtgcttcttc cattaactca cgcaattcag ggtcaagtct gaactggaat ggtggattac
      841 cacgtctttc gttcttgtgt gttgacacgt caattacacc tattgtaatg tgcttatgtg
      901 taatgacaca ttacacacaa ggaatcaaaa tagcaacgcc ccggagtgtt tgcggcactg
      961 ccgaggcgtc tgaccaaagc gttagatgag gtaacgaaaa tggctaaaca gaagtgtacc
     1021 tggttatttg cggcgatcaa ccgcagtcaa cgcaatgccc gtcctgtgat gttaaggatc
     1081 accgcagata acgagcgctc agcgcggcgc agacttgccc cggactatgt gctgagcttt
     1141 gccggtcgca ttccctgtgg aggtgaacat gcgtaattat ccgcaacccg gcgaacgctg
     1201 gcagcatgag cgtggctgga ctgtcacgat tatcagatta actgaagcct cgccctccgt
     1261 ggcgctggtt aaccctgaat tcagctgtga agtgctgatt cgtcatgaca gcgacaataa
     1321 gctttcttcc tgccctctgg catggtttga gcagcggtat acgcgcctgt ttaatgcccc
     1381 gctctttaaa ccttcgctag ctcccgcggg gggtagcggc tctggctcgc tgacgctgca
     1441 tccgtcggtg gtatttaccc gctggcggga gcgcagcata cggcgttctg ctgaacctga
     1501 cgacagtcac tattcaaagt ttctctgaca aaacaccgct gaaaacggag taatccacga
     1561 tgaatatttc aaacgggcac aacggtgctc agggccacgc tttgcctgaa aaacacaata
     1621 gcggtgcttt tgcgtatggg ttgtcagagg acggtttcgg aaagctggac cgcgccagaa
     1681 aggcctgcga catgttgcag cttctctttt ctgaataccc ctcgcagggc ggaacgctgg
     1741 atgcaggatg tgcgccaggc atcgccgcgc tgatggagta cctgcgggca gatttgacgg
     1801 atattgcgca cagttgcgaa ctgattgaag gaggcaccca atgaaccagc cgcaggtttc
     1861 tatttttccg gcagaaatga ccactgcgct ttaccggcgg gctattgcat cggcctggcg
     1921 ccagaaagcg ctgaccgaaa ccggctgcga tcactatggt ccgcacagcc tgacggttga
     1981 acgtattgaa atggccattg ccctgcatat cgagtgcgca ctgattaacg aatatggcga
     2041 agcacaggga gcagcggctg cactggcgct gctgactgac atgctggagc catcattgct
     2101 gaccgcgcca ccggtactga ctgtgcgcgg ctgtgaagtg atggcggagc tgtaccgcac
     2161 acttccggcg gcatttgatg acttctgcag ctcgggtgta tcgctgtatc agggggaaat
     2221 atgacaacac agacggttac gcagatttca gccgcagcgc ggggaaaatg gcccgtcatt
     2281 ctgcagatgc tgcgtattga tgtacctgaa aacggcaggc acggtccctg cccgaaatgt
     2341 gggggcaagg atcgcttccg cctcgatgac ctcgacgggc gcgggacgtg gatttgcagc
     2401 cagtgcggca atggtgatgg tctggatctg gttaagctca taaccggtta cggcatcaga
     2461 aaggcagcgc aggaggtggc gcaggtgctt aatgtgccgg atgtgcagga actgcccgtt
     2521 aagcccgcca ggcaaaaagc ccctaaacgc gatatgagcc ttaccgtggc ggccctgatg
     2581 aaagagagtc acacgggaga aagcccctat ctgaccggaa aagggttcgc cggatacccg
     2641 gcctccctga ccgggagcgt gcagcatatc agtggtaagg atttccctgc cggttccctg
     2701 ctgttaccgc tcacgacgaa cgccggagcc gtgaccggtg cacagcttat cgccccgacg
     2761 ggtgaaaaaa gcatactgcc cggcagcacg atgaaaggcg cgtttgtggc gctcagcccg
     2821 ttaccgtctg aaccgccggt acaggtggtg attaccgaag gttacgccac ggcgctgacg
     2881 gtaagccagc tcaccgccgg atgcgtagtg gcggccatat cggcgggcaa cctgcccaac
     2941 gtcgcacagg cgctgcgggc acgctggcct gaggtaaaaa ttatcatcgc cggtgataac
     3001 gattttcagg acgggggcga gaatcccggc agatcctttg ctgaacgggc ggcaaaatca
     3061 gtcggtggct gggtgacgct gccaccggga gaaattaaag ctgactggaa tgacttccac
     3121 cgtgagcacg gtattacacg tgcccgtgaa gcctttcgta acggtcttgt actgtgcgga
     3181 gaaggccgca cgcagttgcc gcacgggttc cgtcttaccc aggaatatct ctggtatgaa
     3241 aaacaggtac agcgcaacgg tgagacggag atccagaacg tcaaaatatg caacccgttg
     3301 cgcgtgacgg caatcacctg cgatgctgat ggcggtaatt tcgggcgact gctggagtgg
     3361 gaagatacat ggggagagcg ccgccgctgg gcgatgccga tggaaatgct cagtggtagc
     3421 ggtgaggaac tacgccgggt actgctggtt aacgggctgt cctatatcag caccaccggt
     3481 gaggcccgcg cccgcctgat ggaatatatc tcgctgtgta aaccggaacg ccgcgtgacc
     3541 tgcgtcagcc gcactggctg gcatggtcag gtttacgtcc tgcaggatga ggtcagcggt
     3601 gaaggtgcag agggtgtcat tctccagacc acttccgtgc aggggcgcga tttccgtgtg
     3661 tcaggtacaa cggaggaatg gcgggagcac gtttcccgtt actgcaccgg taactcccgc
     3721 gtggcgtttg ccgtcagtct ggcctttgct gcacccctgt tacggctggt tgggatggac
     3781 ggcggcggct accacctcaa gggggaatcg actgacggta agaccaccac catgaaagcg
     3841 gcaacctccg tttgtggcgg gcctgattac tggcagacgt ggcgggcaac cggcaacgcg
     3901 ctggagggat gcgccagccg ccgcaacgat gccgccatga tgctcgatga gatccgggag
     3961 gtggacggac gcgaggcggg taatatcgcc tacatgctgg caaacggtca gggcaagggc
     4021 cgtgccggta cggacggtga gctgcgcacc cgtaagcagt ggcggctgct gttcttttca
     4081 accggcgagc tgtcactgac cgaacatgcg gcaaaagccg gtgagcgtac ctttgccggg
     4141 atggaagtca ggatgatcca gatccccagc gattccggga aatttggcgt atttgaggag
     4201 ctgcacggct tcgacagcgg caaggctctg gcggaacatc tggagtgggc cacgtccagc
     4261 tactacggtt cgccgttcag ggagtggctg aaagccctga ccgctgacct taacgggctg
     4321 acggcacagg caaagtcact gatgaaggaa tatacagccg ccctgacccc gaaagatgcg
     4381 ggtaatcagg taggccgggc tgtgaaccgc tttgcactgg tggcgatggc gtgcgagctg
     4441 gcaacccggc tgggtattac cggctggcct gagggtgaag cgctgcgggc aacccgcgtc
     4501 tgcctgaacg catggctgaa agatcgcggg cacaccgcca atcaggaaga tattgccgca
     4561 ctggagcagg tacgcagctt ctttaccgcg aatcagtaca gccgctttgc tgactggcac
     4621 gacgagcgta accgccccgg caatatggtg ggctggcgca gggtggagaa aggcagtacc
     4681 gcgcagggca cggaggccgt caccacgttc tacgtcatgc cgtccggctg gaaagaaatc
     4741 tgccggggat ttgacccgcg caaggtggcg cgtctgtgcg cggatcgtgg atacctgctg
     4801 ccctccaccg atggcaagct gcagacaacc attcgcccgc cagagatgaa tccccgcaga
     4861 ctctatgtct tcaacagcga ggtgccgggt taaggctttg cgtgagtctt atttattaag
     4921 ggtaacaggt gaaacaggtg gaacacccgt attttacaag ggctaaacct gtttcacctt
     4981 ctgaaatagt gaggtggaac aggtggaacg ccagccgatc cgctgttcca ccttgttacc
     5041 tgcgatgttt cacctgtgat gtgcatataa atgttttaca aaacaatgct gtaacacctg
     5101 ttccacctgt ttcaccgcat ccgggacata aagcaggctg aaacagaggc atttttatct
     5161 ggctggctgt catggccgcg tctgaataac cgcaaacgcc cgttgtgcca gccaccacac
     5221 actgagcgcc gatagtgcgc ttgtgtcagt cacgacacaa ttgacgtaac gaaccaaccc
     5281 ggcaggagaa gtcatgaaca acaccgcaga gaataacaac ctttccactt ttccggctgt
     5341 aacgcagcag gcaatggaaa ccctgaacac agccagaagc gcatggcttg aagcacgtcg
     5401 tcggcagaaa gcggcagcgg ataatattgc gacaatccgc cagcgccggg ctgaaatgga
     5461 agccacgacg aacgcgctga atgaggagtg gcgcacgctg tttcgtgaaa gtcagggcgt
     5521 agtctcaaag gaaatgaaaa aactgcgcac ggaaattgcg ctgggacgag aaacgcttga
     5581 ggattttgat gaactgctgg cagctcagga aagcgaaaat gccttattac cgcaggaagc
     5641 cgggaaatta gccgggcagt atatcagcgc acataacacc cttgtggaaa ttcgcgcaaa
     5701 gcaaatctgg gaagacttca tgcaggcgca tggtaaggcg ttaatccaga cactgagcct
     5761 gctcaaaacc acaatggggc gggaagccag tgccgttgtg ggggtggtga attcagttaa
     5821 tgacccggac acactgctga aagactttat tcacaaacat atcaccaaac cggctcttgc
     5881 taacactgcg atgccggaac tggaccctgt gtttaaactg gcgggcgttg cgccggatta
     5941 tgcggctcgt gtcgatttaa gcaaaatccc gtcaccggcg gcccttcaca aaatgaagat
     6001 ccgtcaggaa cgtgaagagc tgcagaaacg taaccgtgat atggagggta tctgatggcg
     6061 ctgaaatgtc ctgaatgcgg caccgttgca cacgccagaa ccaccgccta tgaagcaccg
     6121 tcggttaaac gctcatggta tcagtgccag aatcttgaat gttcctgcac atttaccgct
     6181 ctggaaagtg tggataagat aattatgaag ccccggcgca atgaacagga atcagacaaa
     6241 gcacaaatgc cggaaaaaca acagcaaacg ctcaatcgct atggctctgc atcaaagtta
     6301 tcaagccgcc agcaaatccc tgtctgatta gcaaaaaacg cctacctaat cccggcccgg
     6361 caccgggatt ttttacgcct ttttcctggc tggcctgaga gcgcatgagt gcatgtctat
     6421 ggcgcatgaa aacgcatgag tctcatgcat catttttgac gtgaaagccc ttgtgtggcg
     6481 gctcctgagg cgatttacgg ggtgcatgaa aaccattctg ttaagcgaag cgggcaggcg
     6541 ggcggggctg ctcgcgctga ggggagatct agggaactta cgttaatcgg tcttgaacac
     6601 gctttaacct atatcatatg attatgctct gatgttggta tctgcgtcta atgtctttct
     6661 ttca
//
LOCUS       DAPGRN010000188         6641 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPGRN010000188 DAPGRN010000000
VERSION     DAPGRN010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.190"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            147..875
                     /gene="gadW"
                     /locus_tag="RHW33_004440"
     CDS             147..875
                     /gene="gadW"
                     /locus_tag="RHW33_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV1647872.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNDSG
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1238..4351)
                     /locus_tag="RHW33_004441"
     CDS             complement(1238..4351)
                     /locus_tag="RHW33_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV1647873.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLHCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4376..5533)
                     /gene="mdtE"
                     /locus_tag="RHW33_004442"
     CDS             complement(4376..5533)
                     /gene="mdtE"
                     /locus_tag="RHW33_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV1647874.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQIQGSTPVQLNLENGKRYNQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5593..5871
                     /locus_tag="RHW33_004443"
     CDS             5593..5871
                     /locus_tag="RHW33_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647875.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5872..6399)
                     /gene="gadE"
                     /locus_tag="RHW33_004444"
     CDS             complement(5872..6399)
                     /gene="gadE"
                     /locus_tag="RHW33_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV1647876.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
BASE COUNT         1669 a         1612 c         1610 g         1750 t
ORIGIN      
        1 atcaattaac tgttatgcaa cactactttg tggataaatt ttggtcctac caaatctggc
       61 agtttttgcg ctaagaaaca gtctggcatc atttcattag tatactgaaa ttgaaataat
      121 cgcagtatga aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc
      181 gttcattcga tatttatcat gaacagcaaa aaatatcgct gcataacgag agtatcctgc
      241 tgctggagaa aaatttggct gacgattttg cgttttgttc accggatacg cgacgactgg
      301 atatcgatga gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt
      361 tagggttaca tagcaaagac cgtttgttaa ttaaccagtc accccccatg ccgctggtga
      421 cggcgatttt tgatagtttc aatgactccg gggtaaattc gccgatactg agcaaaatgc
      481 tctatctttc ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca
      541 atagcatcag tactgtttca ggaaaagttg aacgccttat tagctttgat atcgctaaac
      601 gttggtatct gcgcgatatc gcggaaagaa tgtataccag cgagagtcta atcaaaaaaa
      661 agttgcagga tgaaaatacc tgtttcagta aaatattact cgcctccagg atgtcgatgg
      721 ccagacgatt actcgagtta cgtcaaattc ctctgcatac tattgcggaa aaatgtggct
      781 atagcagtac atcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc
      841 agtttgcgca acattcgcca ggtacctttt cctgacatat ttcgcatttg aatattggtc
      901 aggatctcac acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc
      961 tagttctctt tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca
     1021 tgtttgggat tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc
     1081 tgttcaaatt gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc
     1141 tggtattcct gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg
     1201 ctgaaccttc acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa
     1261 agagatgttc cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg
     1321 caaacatccc gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat
     1381 gactgataac cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc
     1441 gtaaacgcat ccgtgccgct tcgatgattg cctctatcgg cgttttccct tctttctgca
     1501 tcatctcaac ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg
     1561 tcagcaaacc aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta
     1621 atgcgccaac gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct
     1681 cataaagtgc ggcgagggcg aggaacacca cgaccagtga aatcgcatac agcgcaggag
     1741 cctgatttga ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg
     1801 ccggaagttt agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg
     1861 ccgccgcttc acctaaaatc tccattgacg ggatgccgtt gtagcgttcc agtcgcggtg
     1921 aaccataggt ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt
     1981 tgcgtacata ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga
     2041 catacacttt tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg
     2101 cggtggaaat tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg
     2161 cgttgacgtt cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga
     2221 cctgattcgg tgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt
     2281 cgtgccccag gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt
     2341 cagccaccgc gggtaagttg aacgggaaga cgacggcttt attgatactg cttagcgcaa
     2401 tcattgcccg ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccatg
     2461 gcttgagact gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc
     2521 caacggtaaa caccgactgg acattatctt tctctttagt cagataataa tccgtcacct
     2581 gttgtagcac tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca
     2641 taaatacccc ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg
     2701 ccatcccggc gcaaatcagc aggtagacca ccatgtagcg accggtacaa tgcaacagcg
     2761 agcgggtgct atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga
     2821 acagggcgtt aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg
     2881 caggggtcag gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg
     2941 agaactggcg gtagatctcc ccggttgcac cgctcataaa ggccatcggc ataaacactg
     3001 cggaaagaac aacggcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg
     3061 tcgcttcctt cggcggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca
     3121 cgatggcgtc atccaccagt aaccctatcg ccagcaccat cccgaacata gtcaacgtgt
     3181 taatggtgaa accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg
     3241 caatcgtcgg gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga
     3301 cgaggatgat agcctcaacc agtgttttga aaacttcctg aatagaaatt tcgataaacg
     3361 gcgtggtgtc gtaaggataa accgtcttca gacttgccgg gaaataggct gataagcggt
     3421 tcagttcctc tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga
     3481 tggcgatccc ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgccc
     3541 caagttcgac gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca
     3601 acaggatttt gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt
     3661 ttagctgctg gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt
     3721 tttgcacctt aatctgggaa ataacatcgg aaggtaccag gttatattta ttgagttttt
     3781 gcgggtccag ccagatccgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc
     3841 ccgcggtacg gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact
     3901 ggttgaggct gccgttatca gaaataaacg ccgcaaccat caggatatta ctgctcgatt
     3961 tatcgacgct aatcccctgc tgctgcactg cttcaggtaa tgaaggcata gcgagttgca
     4021 gtttattttg cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag
     4081 tgatagaggc attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc
     4141 cattcatatt ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac
     4201 ccggataggt agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg
     4261 gtaagttcat gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa
     4321 aaaccgggcg atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt
     4381 gtttcgattc ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg
     4441 gacgaatgcg ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga
     4501 caacccactg gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acgacatcgt
     4561 ctttatccag aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg
     4621 gcaccagtaa tacattctgg cggctacctt catccactaa tgccgtgaca tacatgccag
     4681 gcagcaagtc accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtagttt
     4741 catccaccgt cgggtcggag aatttcagcg tgccggtctg gttgtaacgt ttaccatttt
     4801 ccagattgag ctgtactggc gtactgccct gaatctgttt gatttgccca ctggcgacct
     4861 cttctttcat gcgtaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca
     4921 gacgttgtac ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca
     4981 ccgacgattt cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga
     5041 tcgtcgcctg ttcaacagcc gctttggcga cggtgacatt ggcttctgcc tcattcaact
     5101 gggtgcgcgc ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct
     5161 ggcggttaaa ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt
     5221 tggcggagtt tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct
     5281 ggttcacttt atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac
     5341 gtatctcggc aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg
     5401 acccagggga gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg
     5461 atttgtcatc gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc
     5521 ttcttctgtt cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc
     5581 gcgaggagag atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag
     5641 gtgggataca ggcatagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt
     5701 ctgccaacag ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac
     5761 caggggaata aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc
     5821 atctgcatcc ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata
     5881 agatgtgata cccagggtga cgatgtcgct catacgtctt agtttcagct tgtacataat
     5941 gttgaatttc tgaatcttta atgtttgttg cgaaatattg agcgtcgacg tgatattgct
     6001 ttcattttta tttttcagaa tcaattgcat aattttgaac tgattatgtg aaaaacataa
     6061 ttgtgaattc ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact
     6121 ttctttgcgg ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg
     6181 tgccaaaaca ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc
     6241 aaggatatga ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt
     6301 gatctctgac agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc
     6361 ctgtaaaaga aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat
     6421 cgtttcgaac atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat
     6481 agtaaacgcc tataagaact ttgcccttcg tgaactaata cgtctattaa cactaatcaa
     6541 tacttcagat ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt
     6601 tatcagcttg taaatagtaa acaaaaaatt aacgtttgcg g
//
LOCUS       DAPGRN010000189         6612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPGRN010000189 DAPGRN010000000
VERSION     DAPGRN010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.191"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(275..2308)
                     /gene="betT"
                     /locus_tag="RHW33_004445"
     CDS             complement(275..2308)
                     /gene="betT"
                     /locus_tag="RHW33_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV1647877.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2437..3024
                     /gene="betI"
                     /locus_tag="RHW33_004446"
     CDS             2437..3024
                     /gene="betI"
                     /locus_tag="RHW33_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV1647878.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            3038..4510
                     /gene="betB"
                     /locus_tag="RHW33_004447"
     CDS             3038..4510
                     /gene="betB"
                     /locus_tag="RHW33_004447"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV1647879.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAD
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4524..6212
                     /gene="betA"
                     /locus_tag="RHW33_004448"
     CDS             4524..6212
                     /gene="betA"
                     /locus_tag="RHW33_004448"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV1647880.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAKEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGSAE
                     LLAEFDIPLVHDLPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKDALPRSTAGYYVANGMPVRAKKMSRDLN"
BASE COUNT         1628 a         1811 c         1798 g         1375 t
ORIGIN      
        1 cattgatttt agttgatttg cgctacgcgt tccccatgaa taaagcgtca tttcaaatta
       61 gcaccaatat ttacattaaa taagactaag cttattgaac attcacaaca gtccaaatgt
      121 aggttttggg cttcaatagt tcttttcttt tttattatct gttgcctggc aatcatcaat
      181 cgatactaag ggcttaaccg tacagatata acgtatcaag aggtaagaga aaccgcgcca
      241 tatgcaaaca tggcgcggtt ttatgcaata acaatcacgc gtccgggaac atcacgctat
      301 ggcccggcgc ttcacgatgg agatgaataa agttaaggtg ccgctcgtac tggtcaagaa
      361 tatcggtgat cacctgctct ttgctgtagt ccatcaggtc gttgccctgg ctgccttcta
      421 acaggaaagt ttccagccgg tagtaagtcg atttaccgct acgtgcgcgg taggtaaagc
      481 ccggcaccga atattgctgc ggccaaatct gatagacaaa gttttgctct tcgcccatat
      541 gcaccaacaa atccagatgc cccaactgtt gtccctcttc cggcggcagg ctttttagct
      601 ccacgtacgc gccgcgcaac cgcagctcct gcgccacttc ttccattgcc gggtaacaga
      661 ccgtctccat catctgttta gtgtaacgcg tgcccggata attcatcagg cgcgagagac
      721 gttttttcca actcaggcga tcctgaagcc ccagcggtcg cggcgcggta tcgcggttgg
      781 cactttcacg gcggtaatct tctactttca gagatttata caaccccgcc atcacgaaga
      841 aaatcacaaa gctgaacggc agccccataa tcaccgtggt gttttgcagc gcggatatcc
      901 cgttggtcat cagcatgccg agcgtcagca ggccaatcgc caccgaccag aagacgcgca
      961 gccagccggg ggcgtcgctg ttgatatctt taagctgcga ggtgaaattc cccagcacca
     1021 gcgccccgga gtccgccgag gtcacataaa acagcaggcc agtaatggtg gcgacggagg
     1081 cgctaaaggt aaacgccgga tactgcgcca gcaggctgta gaagccgcgc tccggatgga
     1141 ccatcgcttc ctctgcaaat gccgcgccgc cgtggatgat ttcatacagc gcgctattgc
     1201 cgaacaccga gagccataac agcgtgaagg taaacggaat aatcaacgtg cccagcacga
     1261 actggcgaat ggtacgccca cgcgagatac gcgccaggaa caagccgaca aacggcgacc
     1321 atgccaccca ccatgcccag aagaagagcg tccagttatt catccactca actggacggt
     1381 cgaaggcaaa actgttgagc gtcatgccca taaagcgatt cacatagtcg ccaacattca
     1441 gcaccagtgc attaagcagg aacgaagtgt cgcccataaa caacacgaac aggatcaatc
     1501 ccagcgccag cgcgacatta agctccgata acacgcgaat gcccttatcg acaccggatg
     1561 tcaccgagat agtggcgatt atcaccgaca aggcgatcag tgccgctttt gccgccatcg
     1621 aatcgggaat atcaaacagt acgctcaagc catagttaag ctgcaccaca ccgataccga
     1681 gcgtagtggc aataccgaag atagtgccga tcacagctgc aatatccact gagtgaccta
     1741 tcggcccgtt aatccgttta ccgaagatcg ggtacagcgc cgagcggatg gtgagcggca
     1801 aattataacg atagctaaag tatccgagcg ccatgcccat cagcgcatac atcgaccagc
     1861 cggttaagcc gtagtgaaac agcgtccaga ccatcgcctg acgcgcggcc tcaatcgtct
     1921 gtcccgcgcc ttccggcggc tgcatatact gcgttaccgg ttcggctacg gaaaagaaca
     1981 tcagatcgat accgatcccg gcagcaaaca gcatcgccgc ccaactcagc aggctgaatt
     2041 ccggtttgga ttgttctggc ccgagcttca ccgaaccaaa acgcgaacaa gcgatacaga
     2101 ccacaaagac aatatagagc gttgccgcca gcagatagta ccaaccgaag gttttagaaa
     2161 cccagtccag cgtgcggcca atccacaggg ccgagaagtc gcgaaacagg atcgttgtca
     2221 gggaaaacaa caaaatcagt ccggcggagg tgtaaaacac caccggattg attttgtcct
     2281 tttccctgct gtgtgaaagg tctgtcatcc agtatcccca ctgttattgt taccatttaa
     2341 aatcaaattc gtaacaatta agacacattt tatattgaac gtccaatcaa taaccgcttt
     2401 aatagataaa caacgctgat gaatggagtg gcgaaaatgc ccaaattggg gatgcagtcg
     2461 atccggcgca gacaactgat cgacgccaca ctggaagcaa taaatgaagt gggcatgcac
     2521 gatgcaacga tcgcgcagat cgcccgccgt gcaggcgttt ctacggggat catcagccac
     2581 tatttcaggg acaaaaatgg tctgctggaa gcaaccatgc gcgatatcac cagtcagctg
     2641 cgtgacgcgg ttttgaatcg attacatgca cttccgcagg gcagtgcaga gcagcgatta
     2701 caggcgattg ttggcggaaa cttcgatgaa acgcaggtga gcagtgcggc gatgaaagcc
     2761 tggctggcgt tctgggccag cagtatgcat cagccgatgc tctatcgttt acagcaggtc
     2821 agcagccgcc gcttgctgtc gaatctggtg agcgagtttc gccgcgaatt gccgcgccac
     2881 caggcacagg aagcgggcta cggccttgcc gcactgattg acggattatg gctgcgcgcg
     2941 gcactgagcg gcaaaccgct ggataaacct ctcgcacact cgctgacccg tcactttatt
     3001 actcagcatt tacccaccga ttaaccgagg agacgtgatg tcccgaatgg cagaacagca
     3061 gctttatata catggtggtt atacctccgc caccagcggt cgcaccttcg agaccattaa
     3121 cccggccaac ggtaacgtgc tggcgaccgt gcaggccgcc gggcgtgagg atgtcgatcg
     3181 cgccgtgaag agtgcccaac aggggcaaaa aatctgggcg gcgatgaccg ctatggaacg
     3241 ctcgcgtatt ctgcgtcggg ccgttgatat tctgcgtgaa cgcaatgacg aactcgcaaa
     3301 actggaaacc ctcgacaccg gaaaagcata ttcggaaacc tcaaccgtcg atatcgttac
     3361 cggtgcggac gtgctggagt actacgccgg gctgatcccg gcgctggaag gcagccagat
     3421 cccgttgcgt gagacgtcat ttgtttatac ccgccgcgaa ccgctgggcg tggtggcggg
     3481 gattggcgca tggaactacc cgatccagat tgccctgtgg aaatccgccc cggcgctggc
     3541 ggcaggcaac gcaatgattt tcaaaccgag cgaagtcacc ccgcttaccg cgttaaagct
     3601 ggctgaaatt tacagcgaag cgggcctgcc ggacggcgta tttaacgtgt tgccgggcgt
     3661 gggcgcggag accgggcaat atctgaccga gcatccgggc attgccaaag tgtcatttac
     3721 cggcggtgtc gccagcggca aaaaagtgat ggctaactcg gcggcctctt ccctgaaaga
     3781 agtgaccatg gaactgggcg gtaaatcacc gctgatcgtt ttcgatgatg cggatctcga
     3841 tctcgccgcc gatatcgcca tgatggcaaa cttcttcagc tccggtcagg tgtgtaccaa
     3901 tggcacccgc gtcttcgttc cggcgaaatg caaagccgca tttgagcaga aaattctggc
     3961 gcgcgttgag cgcattcgcg cgggcgacgt tttcgatccg caaactaact tcggcccgct
     4021 ggtcagcttc ccgcatcgcg ataacgtgct gcgctatatc gccaaaggca aagaggaagg
     4081 cgcgcgcgta ctgtgcggcg gcgatgtact gaaaggcgat ggcctcgata acggcgcatg
     4141 ggtcgcgccg actgtgttca ccgattgcag cgacgaaatg accattgtgc gcgaagagat
     4201 cttcgggccg gtgatgtcca ttctgaccta cgagtcggaa gaggaagtca ttcgccgcgc
     4261 taacgatacc gactacggcc tggcggcggg aatcgtgacg gcggacctga accgcgcgca
     4321 tcgcgtcatt catcagctgg aagcgggtat ttgctggatc aacacctggg gcgaatcccc
     4381 ggcagatatg cccgttggcg gctacaaaca ctccggtatt ggccgcgaga acggcgtgat
     4441 gacgctccag agttacaccc aggtgaagtc catccaggtt gagatggcta aattccagtc
     4501 catattctaa ccaggaggtt tatttgcaat ttgactacat cattattggt gccggctcag
     4561 ccggcaacgt tctcgctacc cgtctgactg aagatccgaa tacctccgtg ctgctgcttg
     4621 aagcgggcgg cccggactat cgctttgact tccgcaccca gatgcccgcc gcgctggcgt
     4681 tcccgctaca gggtaaacgc tataactggg cctatgagac cgagccggaa ccgtttatga
     4741 ataaccgccg catggagtgc ggacgcggta aaggcctggg tggatcgtcg ctgatcaacg
     4801 gcatgtgcta catccgtggc aatgcgctgg atctcgacaa ctgggcgaaa gaacccggcc
     4861 tggagaactg gagctacctc gattgcctgc cctactaccg caaggccgag acccgcgacg
     4921 tgggcgagaa cgactaccac ggcggcgatg gcccggtgag cgtcaccacc tccaaacccg
     4981 gcgtcaatcc gctgtttgaa gcgatgattg aagcgggcgt gcaggcgggc tacccgcgca
     5041 cggacgatct caacggttat cagcaggaag gtttcggccc gatggatcgc accgtgacgc
     5101 cgcagggtcg tcgcgccagc accgcgcgcg gttatctcga tcaggccaaa tcgcgcccaa
     5161 acctgaccat tcgtactcac gccatgaccg atcacattat ttttgacggt aaacgcgcgg
     5221 tgggcgtcga gtggctggaa ggcgacagca ccattccgac ccgcgcgacg gcgaacaaag
     5281 aagtgctgtt atgtgcaggt gcgatcgcct ctccgcagat cctgcaacgt tccggcgtgg
     5341 gtagcgctga actgctggcg gagtttgata ttccgctggt gcatgattta cccggcgtcg
     5401 gcgaaaatct tcaggatcac ctggagatgt atctgcaata tgagtgcaaa gaaccggttt
     5461 ccctctaccc tgccctgcag tggtggaatc agccgaaaat cggtgcggag tggctgtttg
     5521 gcggcaccgg cgttggtgcc agcaaccact ttgaagcagg cggatttatt cgcagccgag
     5581 aggaatttgc gtggccgaat attcagtacc atttcctgcc agtagcgatt aactataacg
     5641 gctcgaatgc agtgaaagag cacggcttcc agtgccacgt cggatcgatg cgctcgccaa
     5701 gccgtgggca tgtgcggatt aaatcccgcg acccgcacca gcatccggcg attctgttta
     5761 actacatgtc gcacgagcag gactggcagg agttccgcga cgcaattcgc attacccgcg
     5821 agatcatgca tcaaccggcg ctggatcagt atcgtggccg cgaaatcagc cccggcacgg
     5881 aatgtcagac ggatgagcag ctcgatgagt tcgtgcgtaa tcacgccgaa accgccttcc
     5941 atccgtgcgg tacctgcaaa atgggctacg acgagatgtc cgtggttgac ggcgaaggcc
     6001 gggtgcatgg gctggaaggc ctgcgcgtgg tggatgcgtc aattatgccg caaattatca
     6061 ccggcaattt gaacgccacg acgattatga ttggcgagaa aatagcggat atgattcgcg
     6121 ggaaggatgc gttgccgagg agcacggcgg gatattatgt ggcaaatggg atgccggtga
     6181 gagcgaaaaa aatgagtcgt gatttgaact gactcaggaa ccgatcagat tgagagttac
     6241 cgttccaggt atggggacag aatcgttata taaaacatag gggtaactct catgcttcat
     6301 agtgacaacc tcgccagcaa attctaagct gactcgttca atctcgctga tgaaggtact
     6361 atgaatgcac tgataaaccg caggtgaaat gccactcagc ttaagaatta cgccgctagt
     6421 gttcctggct atggtgactt gttcactccc gctcacggtt agcatatacg aaaagggttc
     6481 ttgtaacgag gacaaaataa gtcagaccaa aacaacgtgg agctttaaac acaattcgta
     6541 taatttatta gcagtgattt atttttactc ttctttaatt aatcatcatg cttaaatatt
     6601 ttttagcaca ag
//
LOCUS       DAPGRN010000190         6605 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPGRN010000190 DAPGRN010000000
VERSION     DAPGRN010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.192"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..551
                     /locus_tag="RHW33_004449"
     CDS             <1..551
                     /locus_tag="RHW33_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647881.1"
                     /translation="YINKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQ
                     RITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLD
                     EIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKHKYPAAICLALGFFLSI
                     VISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(794..1489)
                     /locus_tag="RHW33_004450"
     CDS             complement(794..1489)
                     /locus_tag="RHW33_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV1647882.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTELNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQASERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1482..2909)
                     /locus_tag="RHW33_004451"
     CDS             complement(1482..2909)
                     /locus_tag="RHW33_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV1647883.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGNVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQICRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2920..3639)
                     /locus_tag="RHW33_004452"
     CDS             complement(2920..3639)
                     /locus_tag="RHW33_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV1647884.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGHKVKVMHIAEVLMSR"
     gene            complement(4167..5021)
                     /gene="rclR"
                     /locus_tag="RHW33_004453"
     CDS             complement(4167..5021)
                     /gene="rclR"
                     /locus_tag="RHW33_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV1647885.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGMDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5247..6572
                     /locus_tag="RHW33_004454"
     CDS             5247..6572
                     /locus_tag="RHW33_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV1647886.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTLGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1799 a         1626 c         1449 g         1731 t
ORIGIN      
        1 tgtatatcaa taaagataat ataaaagtga gttacagcct aaaaataaat cagacaacac
       61 caggcatact tgttaactgt gatagcaata ataactttgc ctgtcagacc acaatgactg
      121 aagatgttat tcagcgaatt actacatttt ttcacaccag cccagatgtc aagaacagag
      181 aaataaagct ggaatggtca ggagataaga gagatttacc aactgctgaa gcggaaatat
      241 ctcgcgtgca ggcgtctatt atcaaatggt atgcgtcaga ataccataat ggcaggcaag
      301 ttctcgatga gatacaaacg ccttcagcaa ttaacagtga gctttataca aaaatgatat
      361 acctgaccag gaactggtca ttgtatccga acggtgatgg ctgtgtaact ataagctcac
      421 cagaaataaa acataaatac cctgctgcca tttgcctggc tctgggattt tttctaagca
      481 ttgtaatttc tgtaatgttt tgccttgtca aaaaaatggt agatgaatac caacaaaact
      541 ctgggcagta ataaacagca ttctgataga gagtgcctgt ataaagctga agtgacaatg
      601 accgggaagg gaacccgttt tcaggaatgg atgcctgtct ttttcactgt aaatatcctg
      661 gcattagtaa tgccaggata tttacagaag aggagcataa ccatgacgga acggggttaa
      721 gtcaggttgt ccttaacgct attcacaacc tcgtatgccg ccttcaggtt tatgtccaga
      781 cttcatatct ctctcagcaa tcctcaataa tcagatacac cgctttcacc gggccatgaa
      841 ccccgacgac tttgataagc tcaatatccg ccgttgaact agggccgcta atgatgttaa
      901 tgcaggacgg catgcgttca ctggcctgcg ctttctgatg caatttttct gcgagttgcg
      961 ctacacgcgg caggattgtg cttttacgca ggataaaaag agaagattcc gggagcaggc
     1021 tcaatgaacg cccgcgctcg gcggcggaaa aaagaaccac gcctcccgat tcggttaaac
     1081 catattcagc atacacgaca cccactttag cctgctctgc ctgcgagata ttctcggcac
     1141 ctttcgccgg atcccaaaca acggcattgc attcctgctg caaacgttcg ctaatcccca
     1201 attcctccag cctcgtgtca ccgctaatca cgaccgactg atctcccagc tctttacaca
     1261 gacgtattgc agcttctgcc gccttcgcct cgctggtcag ctcacagcgc gtcaacataa
     1321 catcgctggc aaactggata aacgcgtcac agcgctgctg ttggttaagt tcggtaagcc
     1381 gctcgttagc atagttatta agcggcgcat cttctgcttg cggttcaagt cgcagcgggc
     1441 gacccagtgc ctgagcaacg ttattcaaaa attcgcctcg attatccatt ctttttctcc
     1501 tgcgcctgat gtttcttaaa ccaactacgg aaactctctc cgtcagcttc aggaagatcg
     1561 cgtgcttcca tccagtcgct aatcgcgcca aatttgagtg gtgttttgcc gccattgata
     1621 aaccagcttg ccgcatgagc gccggccatc atcccgactt tccacaatcc tggatgacta
     1681 ttggcataag cgaacatttt tatcgcccgt tgctctgctt ttgcggtgat ccctttttca
     1741 gccatcaccc gacgatgacg caaaatcagt tttgacagcg gaatacgcac tggacacacg
     1801 ttgtcacaag ctgtgcataa agagcaggcg tagggtaaat ctttaaaatc tttatagccg
     1861 ccaagtagcg gagaaatcac cgcaccaatt ggccctggat aaatagagcc atatccatga
     1921 ccgccaatat ggcgatatgc cggacaagta ttcatacaag ccccgcagcg aatacagcgc
     1981 agcacatccc gaaattcaga ggccagcacc tcagaacgcc cgttatcgac aataaccaga
     2041 tgaaactctt caggaccatc aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg
     2101 tatcccgtca aacgtgcacc aacggcactg cgcgccagca tggtgatcaa tacatctacc
     2161 tcggcaaacg tgggggcaat acgctccatt cccattactg caatatgcgt tttaggcagc
     2221 gtggtacaca ttcgcgcatt accttcattg gtcaccaggc ataccgaacc ggtctctgct
     2281 accgcgaaat tacagccggt aatacctatt tcagcactga ggaaatcttc gcggattttt
     2341 tgccggatga ataaggtcat cgcttcaggc gtttccggcc cctcatagcc cagacgttcg
     2401 tggagcactc gacagatctg atggcgatct ttatgaattg ccgggaccac aacatgagat
     2461 ggcggatctt gatccagctg gagaatatat tcacccagat cggtttcaat cacctgaatg
     2521 ccagcatcct gcaacacatg attgacacca atctcttcgg tcaccatcga tttagatttc
     2581 accaccttcc gggcattttt gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct
     2641 ttggtttttg caaaatagac gttaccgccg ttttgcgtca ctttttctga gagctggtac
     2701 agataagcgt cgagattact cagaacatga tcacgtatct gggcggcccg atcgcgccac
     2761 tcctcccagt gccccaattc atcgaccatt ttttgccgat ttgccccaat acgctgctgc
     2821 gcgtttgcca ccgctttgcg catgatcgga tcttcaattt gctgacggat gcgtgtctta
     2881 aaatctgtat tactggtttt gatcgacatc tttatatcct cagcggctca tcaacacttc
     2941 agcaatatgc atcactttga ctttatgccc ttcccgttgt aatcgcccac tgatgttcag
     3001 caggcaactc acgtcagcac caattaaata ctcagggcgg acttccatca ggtgcgcaac
     3061 cttttctttc accatctcgc cggatatttc ggccattttg accgagaacg tgccgccaaa
     3121 tccgcagcag gtatcctgtt cagcaaaggt caacagctcc agtccacgca catttttcag
     3181 cagcgtaagt ggctcgtcct tcactcccag cttacgggcc aggctacaag atgggtgata
     3241 caccgctctc ccttgcaaac tggcaccgac atcgactacc cctaatttat taacaataaa
     3301 agaggtgaga tcctgcatac gcgcggcaac ctttgcggca cgtgatgccc attcaggttc
     3361 atccgccaga tacatcgggt aactttttac ggcataggtg caggagccag ccggtgaaat
     3421 aatgggatca tcgttatcct ccagtgcggc gatcagattt ttcatccctg gaatcgcttc
     3481 tttgatataa ccgctattga tcgcaggctg accgcagcat ccctgtttct ccgggaaatt
     3541 tacgcgacag ccgagttttt ccagtagcag cacggagtct cgtgccattc ttgatttcag
     3601 ggcgtcacca atacaggtga caaagaaatt gacattcaca actaatactc cattacttca
     3661 tgcccattta ttcccgatgg ttcccccctt ccgggggatc aatcatcaat aaatatggcg
     3721 ttctttttta attggctagc aacataccac cccttatttg tatgacaaat gataatcttt
     3781 catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
     3841 gggaaatcga cgatcatttt tggcataaga aatccatttt aaaatggcta catcccttat
     3901 acctattcag agctacttat tcaggggtta ttgagatata cactgatggt gtacaaaaaa
     3961 atcaccgaca ataatcacgt atcaagtaat agtcgggatt ttatttaatt gaagcaattt
     4021 agtttgcatt tttatttaaa aactgagcta gagtccatca gaaaaaaata tataaagccc
     4081 aaaccaccgt tggaaataga aatagttgaa aggctcacaa ctcccggaat gcggattatc
     4141 tttgccctga tttctggcgg ttttattcag ggtgcaagct gtctgaccct ttcccgatat
     4201 tctcccgggg tacaaccaaa ctcgcgaaca aacgccttgt gaaaagatga ttcactggca
     4261 tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc
     4321 gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg
     4381 aaaagctggg caaaacttgc ccgggacatg tgggcgatgc tggccagcga ttcgacggtc
     4441 caggcgtgtc ctggcatttc cagcatttgc tgtattaccg caccaagacg tggatgcaga
     4501 agcaaactga gaatgttttt ctcagtatta acctgtgcaa tccactcacg caccgcgagg
     4561 gtaaagaatg tagcgcagat ctggctacac agtgcatcca ttcccggcat tgccgatctg
     4621 gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac gctgtggtta
     4681 accggtgcta aaaacagcgt ttccggcaaa gacgttaaaa aataacgtgc cgaatgttgc
     4741 agccgaagag taccacagac aatacaggtc gattcattat cgacatgact caggcgatgg
     4801 gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctcccccgtc
     4861 ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgacaattcc
     4921 ccggcaccat gtggaagttg ccagtcactt cctaacacgc aattcttatc gatcgttcct
     4981 tgtggagcgt taagcatcaa caaacggcta agggcatcca tatcagactc ctgagtggtt
     5041 tttgattaca aacaccaaca atcttagaca ataattaatc taataccatc aaatggttaa
     5101 tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa ataccagacg ataagacaaa
     5161 atatcaagcg ttgtgaagaa atgttatttg ctcttttgcg tctatagtca tgatgtcaaa
     5221 tgaacgcgtt tcgacaggaa atcatcatga ataaatatca ggcagtgatt attggttttg
     5281 gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc agggtggcgt gtggctctca
     5341 tcgaacaatc aaatgcaatg tatggcggga cctgtattaa tatcggctgc atcccaacca
     5401 aaacattggt tcatgacgca cagcagcaca cagattttgt ccgtgccata cagcgtaaaa
     5461 atgaagtggt taatttttta cgtaataaga attttcataa tcttgcggat atgcccaata
     5521 tcgacgtgat cgacggccag gcggagttta tcaataatca tagcctgcgt gttcatcggc
     5581 ctgagggaaa tctggagatt catggcgaga aaatttttat taatactggt gcacaaaccg
     5641 tggttccgcc aattcctgga attaccacca cgcttggtgt atatgacagc accggattac
     5701 ttaatctaaa agaattgcct gggcatttag gtattttggg cggcggatat attggcgttg
     5761 agttcgcctc tatgttcgct aattttggca gcaaggtaac cattttagaa gcagcttcgc
     5821 tgtttttgcc tcgggaagaa cgggatattg ctgataatat cgcgacgatt ttacgcgatc
     5881 agggcgtcga tattatcctc aatgcccatg tggagcgaat cagtcaccat gaaaatcaag
     5941 tgcaagtgca tagcgagcac gcccaactgg cggtggatgc actgttaata gcttccggtc
     6001 gtcaaccggc taccgcttcg ttacatccag aaaatgccgg tatcgcagta aacgagcgcg
     6061 gggcaattgt cgttgacaag cgattacata ccaccgcaga caatatttgg gcgatgggag
     6121 atgttaccgg cgggctgcaa tttacttaca tatcactgga tgattaccgc attgtacgtg
     6181 atgagttact gggtgaaggc aaacgtagta ctgatgatcg gaaaaatgtg ccttattccg
     6241 tatttatgac accgcccctg tccagggttg gtatgacaga agaacaagcc agagagagtg
     6301 gtgctgatat tcaggtggtg acattgcctg tagctgcaat tccgcgcgcc agagtgatga
     6361 acgatacccg tggggtatta aaagcgattg ttgataataa aacccaacgt atattagggg
     6421 catcactgct gtgtgttgac tcccacgaga tgattaatat agtgaaaatg gtgatggatg
     6481 ccgggctgcc ttatagcata ttacgcgatc agatatttac tcatccgtcg atgagcgaat
     6541 cactcaatga tctattttca ttagtcaaat aaactcaaaa tcagacgcca gaacaaatat
     6601 tctgg
//
LOCUS       DAPGRN010000191         6587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPGRN010000191 DAPGRN010000000
VERSION     DAPGRN010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.193"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(189..497)
                     /locus_tag="RHW33_004455"
     CDS             complement(189..497)
                     /locus_tag="RHW33_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HDV1647887.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            complement(584..1228)
                     /locus_tag="RHW33_004456"
     CDS             complement(584..1228)
                     /locus_tag="RHW33_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDV1647888.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            complement(1408..2187)
                     /locus_tag="RHW33_004457"
     CDS             complement(1408..2187)
                     /locus_tag="RHW33_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV1647889.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPVERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            complement(2189..2602)
                     /locus_tag="RHW33_004458"
     CDS             complement(2189..2602)
                     /locus_tag="RHW33_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647890.1"
                     /translation="MFFIENEGQTVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            3134..4060
                     /locus_tag="RHW33_004459"
     CDS             3134..4060
                     /locus_tag="RHW33_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647891.1"
                     /translation="MSEKEHDMTNDGGESVTYTLRNIPADTDRVITDLASHARKPKAT
                     FLREFLEDSFRDVIDSFALKNPLIASLDEELASYLDAKVMEQRYQSHFITRWNQEYQK
                     LLGISTEEELRRLVLNNTPFLQVRADQVLKGWKNIPRGISLTFSLFAEIAGRDRETID
                     QAWKNIFYSQLREKKHRFYQDIEAIRALKKLPALTGDSWTRDGITVRIYRPENYARGA
                     WRVTLSLPENYATQMWNIPFPELEYRLFTADPGYSALISAEPDRWDKAFRFVDGVCEL
                     HLYTNGVEEDHNPTPLGDVAQALINVVEENLL"
     gene            4105..4359
                     /locus_tag="RHW33_004460"
     CDS             4105..4359
                     /locus_tag="RHW33_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647892.1"
                     /translation="MKGRQSRYVTGGESFAEIARLPSGAVVRLCLNTGLEDALREASK
                     SLKSAFTRSGRKCRLSAGTAQGPFTGRRQGVATHLFVSVL"
     gene            4858..5202
                     /locus_tag="RHW33_004461"
     CDS             4858..5202
                     /locus_tag="RHW33_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HDV1647893.1"
                     /translation="MKNLLDTEGTVLFPFQTEIHFIWTIFSTVKRLVIGTRDHICQKQ
                     YWSACLCILLLMAYVGLCAAVVWFVVPWLKAFIVSKFAVSALWVTPVKRGLLWGIGKV
                     VTATCRRFTLLK"
     gene            complement(5378..6379)
                     /locus_tag="RHW33_004462"
     CDS             complement(5378..6379)
                     /locus_tag="RHW33_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HDV1647894.1"
                     /translation="MADPFNLQTDVVRQHTVPRFLLKHFSTPGKGKRQRLYAFDKAAG
                     RAYATTPDDATVRNTFYNLDNHPDRLSLEPLLGIYEHHAAPVIAALLAHRDIRRLTDD
                     ERYRLAVFVAVQRARTFGELERISGMISVLTDKMEAIGSTKEQAMETLGLSSGGDTKD
                     IFLRQLVQQVSHIDLLLKKDWYLLETKPERPFYVSDNPVVLKNSNDFGPYGNLGLAVR
                     GIQIYLPLSSTLMLAMYCPSIREQMVRQKQHLQHLLARAPHLIPRHIRPFERLEHIRR
                     YTDYLLMPLTPEHVTHYNSLQVEFAEQYVFCGEKDFSLVERMLADSERYRTGPRFTF"
BASE COUNT         1660 a         1723 c         1677 g         1527 t
ORIGIN      
        1 atcgtggaca tcatggttca ggttaaggga aaaggaatga aatggtgaaa ctgagaggaa
       61 aggaaagcgg tgacgaatca tgtcaccata aacagaaaga tgtctaatgg ggactcttta
      121 ggtacgtgaa gaatccccat aacgcattta aaagacacac atcagatacc cttaaggtat
      181 ccgatagttt attgacctat tgcaccatca cgttcaagct tctctcttat agcctcaata
      241 atatatgagc taaaatccag agatgtttta ctggtcgctt taagttccgc atgagcttca
      301 aaatacgaca gcggtatggc cttaagattc ttagccttcg tcctggcagc cacgggacga
      361 cctgaaactt cctctgcacc ttcatttggc tgagacgggg ctgttgctgt tacggcagaa
      421 gaagaagttt gtaatctggc agaatccacc aggtttttca gtttttcagc ctgtgcacta
      481 aatttcgctt tgctcatgag ataacccagg atgtttgtta gatacttaaa agatatccat
      541 taaaagccca aaggatacta tatggatacc caatagacac accttactgt ttgttatttt
      601 caatcagatg attgatttct tcgatcaggg caataacttc cttacctcct gccgaatagc
      661 gcccatgaac agactcagtg attcccatcc cagtctcaat gacatcctca aattctgcac
      721 gagcagaaat gcggctcttc atcagcttca gatgtttgaa cgatggcagg attgcatcaa
      781 gcttagggaa tttcttcttg ttaggattaa ctttacagag atacaggtga gcagttatat
      841 cggtccccat gatagagctg atttcgtcca gtactccatc aaaatgcatc aaaccaatac
      901 gttcagtcag ggagtctttt gaaggaacaa taacgcagtc tgcaaaggca atcgcagtac
      961 gggtaagatc tgagtcaaat cccccacagt caataagtaa cgtcttatcg ctgttcttgt
     1021 agggagtcat aatttcgatt aactcatcca ctgttttggg tactttcacg gaaatttctt
     1081 ttccttcagg tctcagcccc agaatgatag aaagcccggt atgagtatcc agatcaactg
     1141 tatcagactt catttcacca acaatgttta cggatgaagt tgtcttaccc acaccacctt
     1201 tgggatgcgt attagcaata acaggcatag tgtatctcca gaatccacat gtaataccca
     1261 taaagacccc ataagacacc tttcggatat ccaagaggta ctcaatagat gtttttttac
     1321 tctataccgg tcctgcatgt caacagaata tgaaataccg ataatatatc catgagatat
     1381 ccgaaaggga tttaaaaaag tcagagctta tggaattctt ttgagcatag acaccgcatc
     1441 ggactcaggc atcgaaaact gcacccggtg ccgtgcagcc acatccagtg caaacacctt
     1501 cgtgtagacc tccgttgagc tgatggactt atgccccatc agactctgca ggaccttcag
     1561 cggtataccg gcatacagca tgtgcatcgc ataggaatgg cggaacgtat gtggcgtgac
     1621 cgggacagag aacgtcacac catcagcggc agcggcggca accgcttccc caatccaggt
     1681 cctgaccgtt ctgtccgtca cttcccagat ccgcgctttc tccgtcctgc ctgttcgttt
     1741 attacgccgt tccacgggga ttttcagtgt ggccaccatc gtctgcagct ggctgacgta
     1801 ccaggagtcg gagagcggaa ccagccggtg agtctgctga ccggcgggca ttcttcctgc
     1861 cgtcctggcg gctttttcgg tccgttgttt cagggtcgca agctgcacaa acggatacgg
     1921 aggcgtgagc gaaaaatccc cccgcgtcag cgccagtgct tcattaatgc gcgcaccggt
     1981 gttccacagt gtggccagca gcatcttgcg gcgcagatcc gggacgtaat ggagcagggc
     2041 gctcacttca ggggccagca ggtatttggg cagttcatca tgaaccatcg acatctggcg
     2101 gagtgccagc gctgccggat aatcgatggc aacaggaagc tgagcggcat cccgggcatg
     2161 gtggatcggt gccggcaggt gctgcatatc agtgttcact ccacggggaa acatcgggca
     2221 ggttttccac aacccggtac tttcccggat aacggagctg gttaccgcca cgcgtccaga
     2281 cggtgacaac aaaaccccct ccctggttgt tttcggggag caggcgatcg cactgctcac
     2341 tcagcatgtg gatcacgaac ggcgcatcgc tgccgtcttc aaataccagt tccagcgcat
     2401 cgcggccatg gagcgttccc ttactgatga tgacgtactc agctccccgc atctccctga
     2461 gtaaatgttt tgccgcatcc gggacaagca gcctggctgc gccggcattc cagctgaggt
     2521 agacatatcc ggcctgcgcc tgtacagact gccagtaatc cgttccggcg acagtctgac
     2581 cttcattctc aataaaaaac atgggtgact ccttttttct tttccgggca gggacggtca
     2641 tgcgataacg tcatccatca tcactgccct taccgtcacg ccgtctgata acatcatgcc
     2701 gttatcatca tcaccatgat aacaagcatc catataacca cgccgttaaa ttaccggaga
     2761 gccgttcccg tcagatccga ctttccggaa tatcacaact ttccggaagt gtaccacgct
     2821 gaggaaataa tcatatcgct gttttccgcc cctgcgcggc gcatcacagg tcagggagat
     2881 gacacaacat atagtgtcaa gagcagctga attcagcact acatgttgtg cttacagggt
     2941 gtggcgctgt gcggaggggg aggaagatcc gtaacctggg gttttcggat ctcagaaaag
     3001 gaagtgaaca gaaaacagac agcgaagcat acgaattata aaaacgtatg cttcgcagta
     3061 tttaaagaca ctcaaaacca ggatagagta taagtattgc agcaatgccc cctcacatga
     3121 aaaggtacgc gggatgagtg aaaaagaaca tgacatgaca aacgacggtg gagagagcgt
     3181 aacgtatacc ctgcggaaca tccccgctga caccgaccgg gtgatcaccg acctggcaag
     3241 ccacgcccgt aagccaaagg cgacgtttct gcgtgagttc ctggaagaca gcttccgcga
     3301 cgtcatcgac agctttgccc tgaagaaccc gctcatcgcc tccctggatg aggagctggc
     3361 cagttatctg gacgctaagg tcatggagca gcgctaccag agccatttta tcacccgctg
     3421 gaatcaggaa taccagaagc tgctggggat ctcgacggaa gaggagctcc gccggttggt
     3481 actgaacaac acccccttcc tgcaggtccg tgccgaccag gtgcttaagg ggtggaaaaa
     3541 tatcccgcgc gggatctccc tgaccttctc cctgtttgcc gagatcgccg gccgggacag
     3601 ggaaaccatc gaccaggcct ggaaaaatat cttctactcg caactgaggg aaaaaaaaca
     3661 ccgcttttac caagatatcg aggccatccg cgccctgaaa aaactgcctg ccctcaccgg
     3721 cgacagctgg acgcgggatg gcatcacggt gcgtatctac cgcccggaaa attacgcccg
     3781 cggcgcatgg cgcgttacac tgagcctccc ggaaaattac gccacccaga tgtggaacat
     3841 cccgttcccg gagcttgaat accgcctctt taccgccgat ccgggctaca gcgccctgat
     3901 cagcgccgaa cccgacaggt gggacaaggc cttccgtttt gtggacgggg tgtgtgagct
     3961 tcacctttac accaacggtg tggaagaaga tcacaatccc accccgctcg gggatgtcgc
     4021 tcaggcgctg atcaacgtgg tggaagaaaa cctgctgtaa cggacccgga tctgcgggca
     4081 caactgcatc atcaggagga tgcaatgaaa ggacgacaga gccgctatgt taccggcgga
     4141 gagagtttcg cggagattgc ccgtctccct tcaggggcgg tggtgaggct ctgtctgaac
     4201 accggtcttg aggatgcgct gcgggaggcc tccaaatcgc tcaagtcagc cttcacccgt
     4261 tccgggcgaa aatgccggct gtcagcgggt acggcgcagg ggccgtttac cggacgccgg
     4321 cagggcgtgg ccacacatct cttcgtctcg gtactctgag ggggcagggg gcaaaaaaag
     4381 taaaaatgta ttcgccaggt tgcccggagg tgaaggaaaa tagacataca gcagaacgac
     4441 ggatagcact ttttgctaaa tggacatcag tattactatg ctatagttgc tttaatggat
     4501 aagtgcgcct tgacaaaggc ggtgatttct gttaacatta ctctcatagt attgttccgt
     4561 cccgctccac cccaacaaga tccgtttatt tcccgccaga ctggttatca ccattccggc
     4621 ccggattttt ttggattttt ttccggggag cccccggaac gagcttaaaa tcggtatgac
     4681 aaacaggagg atgcgaatga acacatcata acagagctga aagataaaac attctgtacg
     4741 gcattaacag cgttcacgtg tgtgaggcgc cgggtgcctt ttgacttaaa aacgaggtta
     4801 ttgagatgac caaaatcaag acagttactt ttgtaaatac ttacccggga gggttctatg
     4861 aaaaacttgt tagacaccga gggaacggtt ctattcccat tccagactga aatccatttt
     4921 atttggacga ttttctccac cgttaaacgc ctggttatcg gaaccaggga ccatatttgc
     4981 cagaagcaat actggagcgc ctgtctctgt attttgcttc ttatggccta tgtgggtctc
     5041 tgtgctgcgg tggtctggtt tgtagtgccc tggctgaagg cctttatagt gtcgaaattt
     5101 gcggtttcgg cactatgggt cacgccagta aagcgcggac tactctgggg tatcggtaaa
     5161 gtggttaccg ccacttgccg aagatttact ctgctaaagt aagtagccgc aacgctacac
     5221 gaactgatgg tgaatgtcaa cagatactca ccatctcctt acggcggtgg tccctgtgac
     5281 cactgccgct ttcgcgtggg tgcaacacgg caaaactcct ctgtacaaca ggggctcccg
     5341 ccgtcatttt ccggcacagg tgaggccgga attcggacta aaacgtaaac cgcgggccag
     5401 tccggtagcg ttcactatcg gccagcattc tctcaaccag agagaaatcc ttttcaccgc
     5461 agaacacgta ctgctccgcg aactccacct gcagggaatt gtaatgcgtg acatgctccg
     5521 gggtaagcgg cataagaaga tagtccgtat acctccggat atgttccagt ctttcaaaag
     5581 gccgaatgtg acgggggata agatgcggcg ccctggcgag cagatgttga agatgctgct
     5641 tttgacgaac catctgttca cggatggagg ggcaatacat ggccagcatc agcgtcgatg
     5701 acagggggag gtaaatctgt atcccgcgaa cggccagacc aagattaccg tacggaccga
     5761 agtcattgct gttcttaagc actacgggat tgtctgatac gtaaaacgga cgttctggct
     5821 tggtttccag aaggtaccag tcctttttca gcaggagatc aatatgtgac acctgctgga
     5881 cgagctgccg cagaaaaata tccttggtat cgcccccgga cgacaggccc agggtttcca
     5941 tggcctgctc tttagtcgag ccgatggcct ccatcttatc ggtaagcacc gaaatcatgc
     6001 ctgagatacg ctccagttca ccgaaggttc gtgcgcgctg cacggcaaca aacacggcca
     6061 gcctgtagcg ttcatcatct gtcagcctgc gaatatccct gtgcgccagc agggctgcaa
     6121 ttaccggtgc cgcgtggtgc tcgtaaatcc ccagcagtgg ttcaagactc agacggtccg
     6181 ggtgattatc gaggttgtag aacgtgttgc ggacggtcgc gtcatccggc gtggtggcat
     6241 aggcgcgccc ggcagccttg tcaaaggcgt ataagcgctg ccgcttaccc tttcccggcg
     6301 tactgaaatg cttcagtaaa aaacggggaa ccgtatgctg cctgaccaca tcggtctgca
     6361 gattgaatgg atcggccata actttatctc cctgaaaagc cacgcaagcg catcggtgca
     6421 tctgtattga cggcatgggc agctggtttg cagcttagca ttagaaaaca gaaccgttcg
     6481 ataatacctg tgtttaacgc cctgtcagta ccgatggaat ttaaatctcc ggaaccgccg
     6541 gaaaccggat ctgcgcggca gaaaagacgc aaatgatatg ataataa
//
LOCUS       DAPGRN010000192         6432 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPGRN010000192 DAPGRN010000000
VERSION     DAPGRN010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.194"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            253..522
                     /locus_tag="RHW33_004463"
     CDS             253..522
                     /locus_tag="RHW33_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647895.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            519..800
                     /locus_tag="RHW33_004464"
     CDS             519..800
                     /locus_tag="RHW33_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1647896.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            885..1232
                     /locus_tag="RHW33_004465"
     CDS             885..1232
                     /locus_tag="RHW33_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647897.1"
                     /translation="MPFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVAVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1250..1786)
                     /locus_tag="RHW33_004466"
     CDS             complement(1250..1786)
                     /locus_tag="RHW33_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647898.1"
                     /translation="MRRGQRYRLVFQFPIRERVLERSGEAPVYGPNLRASCRRLEDAG
                     WLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQRAAEVVVLPLVPAAGLPAD
                     GTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEGRVVCLEGDPLEVAQWLQA
                     CHDAGIEVRVQINESSVP"
     gene            complement(1910..3118)
                     /locus_tag="RHW33_004467"
     CDS             complement(1910..3118)
                     /locus_tag="RHW33_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV1647899.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(3099..3371)
                     /locus_tag="RHW33_004468"
     CDS             complement(3099..3371)
                     /locus_tag="RHW33_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647900.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            3440..3769
                     /locus_tag="RHW33_004469"
     CDS             3440..3769
                     /locus_tag="RHW33_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647901.1"
                     /translation="MPWFLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARA
                     PDGKTARVVIETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFKKHMYG
                     VFMHLRY"
     gene            4048..5928
                     /locus_tag="RHW33_004470"
     CDS             4048..5928
                     /locus_tag="RHW33_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B"
                     /protein_id="HDV1647902.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLKTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AINSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     NIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWAFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(5946..6293)
                     /locus_tag="RHW33_004471"
     CDS             complement(5946..6293)
                     /locus_tag="RHW33_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 1B immunity protein"
                     /protein_id="HDV1647903.1"
                     /translation="MKLDISVKYLLKSLIPILIILTVFYLGWKDNQENARMFYAFIGC
                     IISAITFPFSMRIIQKMVIRFTGKEFWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFILCKALSGK"
BASE COUNT         1655 a         1576 c         1626 g         1575 t
ORIGIN      
        1 cctcgtcggg accactccga ccgcgcacag aagcgctatc atggctgaaa gcgggtatgg
       61 cttagcaggg cggggatggg acaggtgaaa tctatcaatc agtaccggct tacgccgggc
      121 ttcggcggtt ttactccggt atcatatgca acaatggagt gccgccttcc atgccgctga
      181 cgcggcatat cctggtgacg atatctgaat cgttatatac tgtgtatata cgtagtaatg
      241 acgaggtgat aaatggcaca ggttaatatg agtttaagaa tcgacgctga actgaaggat
      301 gcttttatgg ccgctgcaaa aagcatggat cgtaatggct ctcagttaat ccgggatttt
      361 atgcgccaga ccgttgaacg gcagcataat acctggttcc gcgaccaggt tgcggcagga
      421 cgtcagcaac tcgagcacgg cgatgtgctt ccccatgaca tggtcgaatc atctgctgca
      481 gcatggaggg atgaaatgag caggaaggtc gccggtaaat gatggagata ttctggacca
      541 tgctggccag ccaggacagg aagcgcattc gtgagtacat tgcggagcag aacctgatgg
      601 ccgccattga actggacgaa cggattggtt attcagccag cagcctggcc gggcagccgt
      661 ataaaggccg caacggtcgt gtggaaggta cccgggaact ggttattcac ccacattttg
      721 ttctggttta tgaggtcgac agccagtggg gaaaagtgta tatcctgcga gtattgcata
      781 ctgcacagaa gtggccatag aggggctgtc cggatatcac agcatgataa taatcaataa
      841 caataagctg tgtcacgttt acatcatgga acggaggtgc tcctgtgccc tttgagcaga
      901 aaaaagcccg ggccattgcc ctgatggaca gcaaaaagat gtggcggagc aattatgccc
      961 ctcctcttct gcgtattctc tggcggctgg gtatccggct tccgccatta ccctttatgc
     1021 cgttctggca ggtcgcggtg ctgacggggg gcttatgggg cacttcctgg ggatgtgcaa
     1081 tgtggtttat ctactggggg ccgtcaggaa tggtggcagg tgaggccatt attataagta
     1141 tcacgggtgg ttttttgttc ggactgctca cggcctcttt ccactggtgg cgcaggaaag
     1201 ttaaccggct gccaccatgg gatgacgtgt aaaccatgca ggcatgtttt tacgggacgc
     1261 tactttcatt tatctgtacg cgaacctcta ttcctgcgtc atgacaggcc tgcagccact
     1321 gcgccacttc cagcggatcg ccctccaggc ataccaccct gccttcttta ttccataact
     1381 gcagacaggt gctgccgtcg agacgcacca caaaatcccc acggcaggcc tgatacgtta
     1441 tgccgttcag ctctaccgcc agatcagtgg cagaagtgcc gtctgcgggc agacctgctg
     1501 ccgggaccag cggcagtacg acgacttctg ccgcacgctg ttctgcccgg aggcggtcct
     1561 gttctgccag cagcagaggc tgtgccacgg cccggccggc gtccgtcagc tccactgcca
     1621 gctgcaggtt cggagcgcgc agggtacgaa gccagccggc atcctccaga cgacggcagg
     1681 aggcgcgcag gtttggcccg tagaccgggg cttccccgct tcgctccagc acccgttcac
     1741 gtataggaaa ttgaaaaacc agacgatacc gctgtccacg ccttatgcca cgtgagcctt
     1801 tctgcttttc cctccactcc ggtggtttct ctctctgcat ggtcgatttt tataccgtaa
     1861 caggctgttt tttatacctg caccgattcc cgggcacact tagcctgtgt caggccggca
     1921 tatacctcag cagactgata tcccgcgcgt ttttcttcag ccctgataca ttcagccccg
     1981 caatggcccg gcggtatacc atccggccgc cacacagcac gcattcgaac gggtcacgac
     2041 tcaggaactg tttcaccatt tgtgcatagc acactttcgc cactggttcc ggcttatcca
     2101 tccccagagc acggtacacc tgcggcagct tctctccaca cacccggttg gcaaggaacc
     2161 cgaagtatct caccatctta aaaaacttct ccgggatgtg ctgtttcagc ctcgcgacca
     2221 gctcccgctg tgtcagcgtt tccgtcgccg tttctcccgt tttgtggtcc aggtaacgga
     2281 agctcagact cgctcctccg ttgtaatgcg ccagccggga agccgctatt ggtggcttct
     2341 tcagataacg acccaggtag cgtgccgtat tccgccctcc ggctgtcttc ttcgacatgt
     2401 acacatgcca gtattttccg ccggctttca gcaccaggct tctccactgt gattccgtcg
     2461 tgatatgtga caacgattcc ggcatcgcca gaccttctga ccacgctttc agaagcagct
     2521 gccgcatatt ccacatccac cgcgaacgca tcgcgtcttt caggaagctc agctttttcc
     2581 agtgtccgtg tttattcaga cctccgcagg tcactgacac atgcacatgc ggatgccagt
     2641 tgagacgacg gccatacgta tggatggcgc agaagatacc gggttccaga ccccgtttcc
     2701 gggctgcata cagcagattc tccaccgcca gacggcacac gtcattcagc agccagcggt
     2761 tgctttcgaa caccggccac agcgtgtccg gcagggtgaa gaccaggtgc acccagtcgc
     2821 agtcaggaag acgattcagc tgtgtcgcta tccacaggtc tgtggccttt tttccgcagg
     2881 acgggcaggc acggctgcca catgagttag tgacgtatct gacgtggggg cactcaggtt
     2941 tatcgcagtt gtattccttc acacccagta tccgcgtgcc gcaggccagc atttttgtga
     3001 cagcctcaac ctcgatatcg cgcaggccgc ccgcatccag gaaggatgtc cagcactggt
     3061 tggctgtgaa caggcgtttc agcggtcggg gctgaaaatc accaccactt cgggtcatcc
     3121 gccttcatct ccgcttcagt ttcgtataaa tcaaaacgac ggcaggtatg gcagaacgtg
     3181 acgtattcgt acggtgaatc cgggtacatt ttgtggaagg tttcccagaa acagaccgta
     3241 caggagggat gaccggcgat aaagtctgta aatacagact gataagggtg attattggcc
     3301 ctggcgacgg ctttcagaac ctctttcacc attctggtgt ggactttctg gtgctccagg
     3361 ttgtgtgaca tgggaactca ttctggatgg ttactctgaa agctcatatt ctgccacacc
     3421 acgatttgca gccgccaggc tgccgtggtt ccttaaaaga aagccaggta cgccggaatt
     3481 aagtctggaa aggcccctgt ttgatacaga agtttatgtt aatggtgaaa agaaatatgt
     3541 actgccggat ttcattgtca cagcaagggc tcctgacgga aagacggcca gagtggtcat
     3601 cgaaacgatg ggatatgaag acagtgatta ctgcgcgaga aaatccaggc agcataccgg
     3661 catgaagcag attggtgttc tgcataccga tccaccgaaa tggctggata acgatcatcc
     3721 cccttttaag aaacatatgt acggtgtttt tatgcatctc aggtactgag atattttgtg
     3781 gctcagttct gtaacttttc ccgtaacatt gtctgttgtt acgggaaagt ccggtttttg
     3841 tattgcacca gagaataccc agactgtgat gctgccacag tgtcagcagg ctttctgaac
     3901 ggtataacac cacttcatgt taatgataat tactatcatt aaatcttgac atgccatttt
     3961 ctccttaata aattagtact gtatatgtat ccatatacgt aagcagttaa ttcatttgtt
     4021 ttcctcagag gatgaaggag ataccgaatg tctgaccctg tacgtattac aaatcccggt
     4081 gcagaatcgc tgggatatga ttcagatggc catgaaatta tggccgttga tatttatgta
     4141 aaccctccac gtgtcgatgt ctttcatggt accccgcctg catggagttc cttcgggaac
     4201 aaaaccatct ggggtggaaa cgagtgggtc gatgattccc caacccgaag tgatatcgaa
     4261 aaaagggaca aggaaatcac agcgtacaaa aacacgctca gcgcgcagca gaaagagaat
     4321 gagaataagc gtactgaagc tggaaaacgc ctttctgcgg caattgctgc aagggaaaaa
     4381 gatgaaaaca cactgaaaac actccgtgcc ggaaacgcag atgccgctga tattacacga
     4441 caggagttca gactcctgca ggcagagctg agagaatacg gattccgtac tgaaatcgcc
     4501 ggatatgatg ccctccggct gcatacagag agccggatgc tgtttgctga tgctgattct
     4561 cttcgtatat ctccccgcga ggccaggtcg ttaatcgaac aggctgaaaa acggcagaag
     4621 gatgcgcaga acgcagacaa gaaggccgct gatatgcttg ctgaatacga gcgcagaaaa
     4681 ggtattctgg acacgcggtt gtcagagctg gaaaaaaatg gcggggcagc ccttgccgtt
     4741 cttgatgcac aacaggcccg tctgctcggg cagcagacac ggaatgacag ggccatttca
     4801 gaggcccgga ataaactcag ttcggtgacg gaatcgctta agacggcccg taatgcatta
     4861 accagagctg aacaacagct gacgcaacag aaaaacacgc ctgacggcaa aacgatagtt
     4921 tcccctgaaa aattcccggg gcgttcatca acaaatcatt ctattgttgt gagtggtgat
     4981 ccgaggtttg ccggtacgat aaaaatcaca accagcgcgg tcatcgataa ccgtgcaaac
     5041 ctgaattatc ttctgaccca ttccggtctg gactataaac gcaatattct gaatgaccgg
     5101 aatccggtgg tgacagagga tgtggaaggt gacaagaaaa tttataatgc tgaagttgct
     5161 gaatgggata agttacggca acgattgctt gatgccagaa ataaaatcac ctctgctgaa
     5221 tctgcgataa attcggcgag aaataacgtc agtgccagaa caaatgaaca aaagcatgca
     5281 aatgacgctc ttaatgccct gttgaaggaa aaagagaata tccgtagcca gcttgctgac
     5341 atcaatcaga aaatagctga agagaaaaga aaaagggatg aaataaatat ggtaaaggat
     5401 gccataaaac tcacctctga tttctacaga acgatatatg atgagttcgg taaacaagca
     5461 tccgaacttg ctaaggagct ggcttctgta tctcaaggga aacagattaa gagtgtggat
     5521 gatgcactga acgcttttga taaattccgt aataatctga acaagaaata taacatacaa
     5581 gatcgcatgg ccatttctaa agccctggaa gctattaatc aggtccatat ggcggagaat
     5641 tttaagctgt tcagtaaggc atttggtttt accggaaaag ttattgatcg ttatgatgtt
     5701 gctgtggagt tacaaaaggc tgtaaaaacg gacaactggc gtccattttt tgtaaaactt
     5761 gaatcactgg cagcaggaag agctgcttca gcagttacag catgggcgtt ttccgtcatg
     5821 ctgggaaccc ctgtaggtat tctgggtttt gcaattatta tggcggctgt gagtgcgctt
     5881 gttaatgata agtttattga gcaggtcaat aaacttattg gtatctgaca cataatgatg
     5941 ccctttcatt ttcctgaaag ggctttgcag agtataaaaa ttaagtaaat ggcaaccaca
     6001 ggaactgata taacgaaaca gaataattca aatattgcag ttaagcttcc gccaactgga
     6061 tttgtaaaga agtctttttg ccagaattct ttccctgtaa accttattac cattttctgt
     6121 attatcctca ttgaaaaagg aaaagtaatg gcactgataa tgcatccgat gaacgcataa
     6181 aacattcttg cattttcctg gttatctttc catcccagat aaaaaactgt aagaataatg
     6241 aggattggta tcaggctttt cagtaaatac tttacagata tatccagttt catctggttt
     6301 ctcctttatt gttatcgtgt accggtgcgt gataacataa tcacagcatg ataataataa
     6361 tcaataacaa taagctgtgt cacgtttaca tcatggaacg gaggtgctcc tgtgaccttt
     6421 gagcagaaaa aa
//
LOCUS       DAPGRN010000193         6411 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPGRN010000193 DAPGRN010000000
VERSION     DAPGRN010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.195"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..2712
                     /gene="traC"
                     /locus_tag="RHW33_004472"
     CDS             85..2712
                     /gene="traC"
                     /locus_tag="RHW33_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HDV1647904.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGVPFCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMNAAATQFPLPEGMNLPLTLRHYRV
                     FFSYCSPSKKKSRADILEMENLVKIIRASLQGASITTQAVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLREKGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKKNRARIDDVVDFLKNARDNDQYVESPTIRSRLDEMIV
                     LLDQYTAGGTYGRYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            2709..3095
                     /gene="trbI"
                     /locus_tag="RHW33_004473"
     CDS             2709..3095
                     /gene="trbI"
                     /locus_tag="RHW33_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDV1647905.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEVQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            3092..3724
                     /gene="traW"
                     /locus_tag="RHW33_004474"
     CDS             3092..3724
                     /gene="traW"
                     /locus_tag="RHW33_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDV1647906.1"
                     /translation="MRCRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     VLEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVLCQRLGIDQVPARVSAVPGNRFLKVEFIPAEEGRK"
     gene            3721..4713
                     /locus_tag="RHW33_004475"
     CDS             3721..4713
                     /locus_tag="RHW33_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HDV1647907.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPIDTLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            4722..5360
                     /gene="trbC"
                     /locus_tag="RHW33_004476"
     CDS             4722..5360
                     /gene="trbC"
                     /locus_tag="RHW33_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDV1647908.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCRQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            5357..>6411
                     /locus_tag="RHW33_004477"
     CDS             5357..>6411
                     /locus_tag="RHW33_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDV1647909.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTC"
BASE COUNT         1523 a         1649 c         1870 g         1369 t
ORIGIN      
        1 gtgccgggaa atggcgcgtt aattacaggt attcccttca tggctaccgc atttctcgct
       61 ttatttttca actaaggaat tcatgtgaat aatccacttg aggccgtcac tcaggcggtt
      121 aactccctcg tcacagcact gaaactgcct gacgaatccg caaaggccaa tgaagttctg
      181 ggcgaaatga gcttcccgca gttcagtcgt ctgctgccgt accgtgatta caaccaggaa
      241 tccggtctgt tcatgaatga caccacgatg ggctttatgc tggaagccat tcccatcaat
      301 ggggcgaatg agtccattgt ggaggccctc gatcacatgc tgcgcaccaa actgccgcgc
      361 ggggtgccgt tctgcatcca tctgatgtcc agtcagctgg ttggtgacag gattgaatac
      421 gggctgcgtg agttctcctg gtcgggtgaa caggctgaac ggtttaacgc cattacccgt
      481 gcctattata tgaacgcggc agcgacacag tttccgctgc cggaggggat gaatctgccc
      541 ctgaccctgc gccattaccg ggtattcttc tcgtactgtt ctccctcgaa gaaaaaaagc
      601 cgggctgata ttctggaaat ggaaaacctg gtgaaaatca tccgggcgtc gttacagggg
      661 gccagtatca ccacacaggc ggtggatgca caggccttta tcgatattgt cggggagatg
      721 attaaccata acccggactc cctgtatccg aaaagacgtc agctggaccc gtattctgat
      781 ctgaattatc agtgtgtgga ggacagtttt gacctgaaag tccgggcgga ttacctgacg
      841 ctgggcctgc gtgagaaggg caggaacagc acggcccgca tcctgaattt ccatctggcc
      901 cgtaacccgg aaatcgcctt cctgtggaac atggccgaca actacagcaa cctgctgaac
      961 ccggaactgt ccatctcctg tccgttcatc ctgacgctga ctctggtggt ggaagaccag
     1021 gtgaaaaccc acagcgaagc caacctgaag tacatggacc tggagaaaaa gtcgaagacc
     1081 tcctatgcca aatggtttcc gtccgtggag aaagaggcga aggagtgggg ggaactgcgt
     1141 cagcggctgg gctccggtca gtcctctgtc gtgtcctact tcctcaacat cacagcattc
     1201 tgcaaggaca ataatgaaac ggcgctggaa gtggagcagg acatcctgaa cagcttccgt
     1261 aaaaacggtt ttgagctgat ttcaccgcgc tttaaccaca tgcgtaattt cctgacctgc
     1321 cttcccttta tggccgggaa agggctgttt aaacagctga aagaggccgg tgtggttcag
     1381 cgcgcagaga gctttaatgt ggccaacctg atgccgttag tggcggataa ccccctgaca
     1441 ccggcaggtc tgctggcacc cacctaccgt aaccagctgg cgtttatcga tattttcttc
     1501 cgggggatga ataacaccaa ctacaacatg gcggtctgtg gcacctccgg ggccggtaaa
     1561 accggactga tacagcccct tatccgcagc gtgctggact ccgggggctt tgccgtggtg
     1621 tttgacatgg gggatggata taagtccctg tgtgagaaca tgggcggggt gtatctggac
     1681 ggtgaaaccc tgcgttttaa cccgtttgcg aacatcaccg atattgacca gtcagcggag
     1741 cgtgtccgtg accagttgtc ggtgatggcc agccccaacg gtaacctgga tgaagtgcat
     1801 gaaggtctgc tgctgcaggc ggtcagggcc tcctggctgg ctaaaaagaa cagggcgcgc
     1861 atcgatgatg tggtggattt cctgaaaaac gcccgtgata acgaccagta tgtcgagtca
     1921 ccgaccatcc gcagccgtct ggatgaaatg attgtcctgc tggatcaata taccgccggc
     1981 ggcacttacg gccggtattt taactctgat gagccgtccc tgcgggatga tgcgaaaatg
     2041 gtggtgctgg agctgggcgg actggaagac cgtccgtcac tgctggttgc ggtgatgttc
     2101 agcctcatta tttacatcga gaacaggatg taccgcacgc cgcgtaacct caaaaaactg
     2161 aacgtcattg atgagggatg gcgtctgctg gacttcaaaa atcacaaggt cggtgagttt
     2221 attgagaaag gttaccgtac cgcccgccgt cataccggtg cctatatcac catcacacag
     2281 aacatcgtcg actttgactc tgacaaggcc tccagtgctg cccgcgcggc atggggtaac
     2341 tcctcctaca aaattatcct caaacagagt gcgaaggagt tcgcgaaata caaccagctg
     2401 tatccggacc agttcctgcc actgcagcgt gacatgattg gtaagttcgg tgcggccaaa
     2461 gatcagtggt tcagttcctt cctgctgcag gtggaaaacc attcctcctg gcaccgcctg
     2521 tttgtggacc cgttaagccg cgccatgtac agctctgacg gcccggattt tgagtttgtg
     2581 cagcagaaac gtaaagaggg gctgagtatt catgaggcgg tgtggcagct ggcgtggaag
     2641 aagtcagggc cggagatggc ttcgctggaa gcctggctgg aagaacatga gaaatacagg
     2701 agtgttgcat gagttcaacg cagaaacccg ccgacgtcac ggcagaacga cgcagccact
     2761 ggtggtggac ggtgccaggt tgtctggcaa tggtgttact gaacgcagct gtcagttacg
     2821 gcattgtcag actgaacgca ccggtgactg tcgccttcaa catgaaacag accgtagatg
     2881 cgttttttga cagcgccagt cagaaacaac tgtcagaagt acagtcaaaa gccctttcag
     2941 cgcgttttaa cacggcactg gaagccagcc tgcaggcgtg gcagcagaaa caccacgcgg
     3001 tcattctggt gtcgcctgct gtggtacagg gggcaccgga tatcacccgg gaaatccagc
     3061 aggatattgc ccggcgaatg agggcagaac catgagatgt cgggggctga ttgccctgct
     3121 ggtatggggg cagagtgtgg ccgccgccga tcttggcacc tggggcgatc tgtggccggt
     3181 aaaggagccg gacatgctga cggtgatcat gcagcgcctg acggtactgg agcagtccgg
     3241 tgagatgggc cggaaaatgg atgcctttaa ggagcgggtg atccgcaaca gtctgcggcc
     3301 ccctgccgtg cccggtatcg gacgcacgga gaaatacggc agccggttgt ttgacccgtc
     3361 cgtcagactg gctgcggata tccgggataa cgaagggcgg gtgtttgccc gccagggtga
     3421 agtgatgaat ccgctgcagt atgttccttt taaccagacg ctgtatttca tcaatggcga
     3481 tgatccggcg caggttgcct ggatgaaacg ccagacgccg cccacactgg agagcaaaat
     3541 tatcctggtg cagggcagta tcccggagat gcagaaagca ctggacagcc gtgtttactt
     3601 tgaccagaac ggtgtgctct gccagcgcct cggcatcgat caggtaccgg cacgggtgag
     3661 cgccgttccc ggcaatcgct tcctgaaggt ggaatttatt ccggcagagg agggcagaaa
     3721 atgaagcgaa gcctgtggct gctgatgtta ttccttctcg ccggtcatgt ccctgcggcg
     3781 tctgcggatt ctgcctgtga ggggcgtttt gtaaacccga tcacggatat ctgctggagc
     3841 tgtattttcc cgctctcgct gggcagtatc aaagtcagtc agggtaaggt ccccgacacg
     3901 gcgaacccgt cgatgcccat tcagatttgt ccggcaccgc cgccgctgtt caggcgtatc
     3961 ggactggcca ttggttactg ggagccgatg gcgctgacgg acgtcacccg gtcaccggga
     4021 tgtatggtga acctgggctt cagcctgccg gcttttggta aaacggcaca gggaacggcg
     4081 aaaaaagatg agaagcaggt gaacggagcg ttctatcacg ttcactggta caaatacccg
     4141 ctgacgtact ggcttaacat catcacatcg ctgggctgcc tggaaggtgg tgacctggat
     4201 atcgcttatc tttctgaaat cgaccccacc tggacggaca gcagcctgac caccattctc
     4261 aatccggaag ccgtcatttt tgccaatccg atagcacagg gggcctgtgc tgcagatgcc
     4321 attgccagcg cctttaacat gcccatcgat actctgttct ggtgtgccgg ttcgcaggga
     4381 agtatgtacc cgttcaatgg ctgggtgagt aatgagtcca gtccgttgca gtcctccctg
     4441 ctggtcagtg aacgcatggc gttcaagctg caccgtcagg gcatgattat ggaaaccatc
     4501 gggaaaaata acgccgtctg taatgaatat ccgtccccaa tcctgcccaa agaacgctgg
     4561 cgttaccaga tggtgaatat gtatccggac agcgggcagt gccacccgtt cgggcgcagc
     4621 gtgacccgct gggaaaccgg gaaaaatccg cccaacacaa agaaaaactt cggctacctg
     4681 atgtggcgta aacgtaactg tgtcttcctg tgaggtgaat gatgaagctg agtatgaaat
     4741 ctctggcagc actgctgatg atgctgaacg gggcggttat ggcgtcagaa aatgtgaaca
     4801 cacctgaaaa ccgccagttc ctgaagcagc aggaaaattt aagcagacaa ctgcgtgaaa
     4861 aacctgacca tcagctgaaa gcctgggcgg agaagcaggt gctggaaaac ccccttcagc
     4921 gttcagataa ccatttcctg gatgagctgg ttcgtaaaca gcaggcttca caggacggga
     4981 aaccccggca gggtgccctg tattttgtgt cgttttccat tcccgaagag gggctgaaac
     5041 ggatgctggg cgaaacccgg cactacggta ttccggccac actgcggggc atggtgaata
     5101 atgacctgaa gaccaccgct gaagccgtgt tgtccctggt gaaagacggc gcaactgatg
     5161 gtgttcagat cgacccgacg ctgttttcac agtacggcat tcgcagtgta ccggcactgg
     5221 tggtgttctg ccgtcaggga tacgacatca tccggggaaa cctgcgggtc ggccaggcgc
     5281 tggagaaagt ggccgccacg ggtgactgcc ggcaggtggc tcatgattta ctggcaggga
     5341 aaggagattc cgggaaatga aacgtatttt acctctgata ctggctctgg ttgccggcat
     5401 ggcacaggct gacagcaaca gtgattaccg ggccggctct gattttgctc accagatcaa
     5461 aggacaggga agcagcagta ttcagggctt taagccacag gagagtatcc ccggctataa
     5521 cgcgaatccg gacgagacaa aatattacgg tggcgtgact gccggcgggg atggcggcct
     5581 gaaaaatgac ggcaccacgg aatgggcgac cggtgaaacc ggaaaaacca ttacagagtc
     5641 attcatgaac aagccgaaag acattctttc accggatgca ccgtttatcc agaccggcag
     5701 ggatgtggtg aaccgggctg acagcattgt gggaaacacc gggcagcagt gcagtgcgca
     5761 ggagattagc cggagtgaat acacgaatta cacctgtgag cgggatttac aggtggagca
     5821 gtactgcaca cgaactgccc gtatggagct gcaggggagt accacatggg aaacccggac
     5881 gctggagtat gaaatgagtc agctacctgc acgtgaagtg aatggtcagt atgttgtctc
     5941 cattacctct cccgttaccg gtgaaattgt cgatgcgcat tacagctgga gtcgtactta
     6001 cctgcagaag agtgtaccta tgacaattac agtactggga accccactga gctggaatgc
     6061 caaatactca gcagatgcct catttacacc agtacagaaa acactgactg ccggtgtggc
     6121 ttttacgtca tctcaccccg tccgggtcgg gaatacaaaa ttcaaacgtc atacggcaat
     6181 gaagctgcgt ctggttgtca gggtaaaaaa agcctcgtac accccgtatg ttgtctggtc
     6241 tgaaagctgc ccgttcagca aggagctggg gaaactgaca aaaacagaat gtacggaggc
     6301 tggcgggaac aggacgctgg tgaaagacgg tcagtcatac agcatgtacc agagctgctg
     6361 ggcataccgg gacacgtatg tcacgcagtc agcagacaag ggaacctgtc a
//
LOCUS       DAPGRN010000194         6371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPGRN010000194 DAPGRN010000000
VERSION     DAPGRN010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.196"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..334
                     /locus_tag="RHW33_004478"
     CDS             <1..334
                     /locus_tag="RHW33_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647910.1"
                     /translation="IQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGILEDIMSIPD
                     SSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAP
                     IQNGPLPA"
     gene            complement(338..1729)
                     /gene="tcyP"
                     /locus_tag="RHW33_004479"
     CDS             complement(338..1729)
                     /gene="tcyP"
                     /locus_tag="RHW33_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV1647911.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHH"
     gene            complement(1862..2452)
                     /locus_tag="RHW33_004480"
     CDS             complement(1862..2452)
                     /locus_tag="RHW33_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV1647912.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2615..3283)
                     /gene="hxpB"
                     /locus_tag="RHW33_004481"
     CDS             complement(2615..3283)
                     /gene="hxpB"
                     /locus_tag="RHW33_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV1647913.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3430..3966
                     /locus_tag="RHW33_004482"
     CDS             3430..3966
                     /locus_tag="RHW33_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV1647914.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4007..4867)
                     /locus_tag="RHW33_004483"
     CDS             complement(4007..4867)
                     /locus_tag="RHW33_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV1647915.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     ANILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4973..5263)
                     /gene="ghoS"
                     /locus_tag="RHW33_004484"
     CDS             complement(4973..5263)
                     /gene="ghoS"
                     /locus_tag="RHW33_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV1647916.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5364..6293)
                     /gene="pfkB"
                     /locus_tag="RHW33_004485"
     CDS             complement(5364..6293)
                     /gene="pfkB"
                     /locus_tag="RHW33_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV1647917.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSLSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1644 a         1588 c         1563 g         1576 t
ORIGIN      
        1 gattcaaaaa aatgagagtc tcctaaagtt tccttcgata atatcaatgc ttaatatcaa
       61 tcttcttttt aatccactaa atcttccggg aatgggaagt ggtatacttg aagatattat
      121 gagtattcca gatagttcac ttagaaaacg tctcgggtat gaggttttaa gcttctcgct
      181 acaggcacat tcattgagcc aggaatgtat tgataagttg gacatttttt tcgctgacga
      241 tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa catttaaaaa gtaaggcaac
      301 ggcccctata caaaacggac cgttgccagc ataagaatta atggtgtgcc agttcggcgt
      361 cgtcttcgct atccagaatg gctttatcgg tttgcttcag ccactggctg gtcagcgtgc
      421 cagctgtcat cgagccacta acgtttagcg ccgtacggcc catgtcgata agcggttcaa
      481 cggagattaa cagcgccacc agggttactg gcaggcccat cgcaggcagc acaatcagtg
      541 cggcgaaagt tgcaccacca ccgacaccgg caacgcctgc ggagctaacg gtaacaatac
      601 cgaccagcgt cgcaatccac atcgggtcca gcgggttaat gccaaccgta ggcgcaacca
      661 tcaccgccag cattgccgga tacaaaccgg cgcagccgtt ctgaccaatg gtcgcaccga
      721 aagaggcggc gaaactggcg atggattcag gaacgcccag acgacgcgtt tgtgcttcca
      781 cattcagtgg gatagacgca gcactggaac ggctggtaaa ggcaaacgtc agcacaggcc
      841 ataccttacg gaagtacttc agcggactca cgccattaat gcccagcaga atgccatgca
      901 ctgcaaacat aatcagcaga ccgaggtagg acgcgacaac gaaacttccc agtttgatga
      961 tgtcttgcag gttagaacct gcaaccactt tggtcattag tgccagaacg ccgtaagggg
     1021 tcaactgcat gaccaggcga actagtttca tcacccagct ttgcagggta tcgatagcgg
     1081 ttaagacgcg ttcacctttc ggcgcatcat ccttcagcag tttcagcgca gctacaccga
     1141 ggaatgcggc aaaaattacc acgctgataa ttgacgtcgg attggctccg gtcagatcgg
     1201 caaacgggtt tttcgggata aaggacaaga ccagctgcgg aacgctcagg tcagagactt
     1261 taccaacata gttactttcg atggcgttca gacgtgcagt ttctgcacca ccctgaacca
     1321 gaccttcagc cgtcaaacca aacaggttgg tgaccagcac accgaccagc gccgcaatca
     1381 gcgtggtaaa caaaagcgta ccgatggtca gaaaactgat tttacctaac tgagatgcgt
     1441 tatgcagacg ggcaaccgcg ctcagaatag aggcgaagac taacggcata acgatcattt
     1501 gcagcagttg aacatagccg ttaccaacga tgttaaacca ctgtacagaa tctttaagta
     1561 cctggctgtc agaaccataa atggtatgca gggcaaggcc aaaaaccaca cccatcacca
     1621 gacccaccag cacttttttc gccagactcc actgtttatg gcgggtctga gccagcgcaa
     1681 acagcagtac aacgaacacc acgatgttcg caattaatgg aaagttcatc cccgttctcc
     1741 tgatcttatt atttctatca ctgcgtagca gtcatagtgt tggcgcaaga ttaacagaac
     1801 tgtgagttgg ctcttatatt caaatggaat tgcttattcc agaaaggtga tttttgcctt
     1861 tttagtattc aacctgatgc ttaataagcc gatgaaactg gatctgcaag gtattgatca
     1921 tttgcgatga ccaacgaaca gcgctgttct cgggtaatga atggggcatc cataccgacc
     1981 agacaaatag tgccatgcag ataaaacgtt ccagttggtt acccttttgg ggaaccagga
     2041 taggcaagcg gaaacgccag cggcatggcc agagcagggg aacaccggcg ggtgtcagca
     2101 tatcggcaag tatgtggctt aaataaccca gcaccattcc ttgtagcgca tcagccggaa
     2161 tgaaccagct ttcaggaacc ttaaggtaaa aggttgccag cagcgcaaat accgccagca
     2221 gactgtgggt aaaaccacga tgcccaaaag cgcgggcgat cggttttgat atccacttta
     2281 atcgctgccc aagaaacgac tttgggtgat cgatgtccgg taacaaacac gtcaggattg
     2341 cggaagggac aatatgccac cagtcaccct gtgccagcac gggcgtcagc tcggcatttt
     2401 tggcaaatac cgcacaagca atagaaaaga gaaggtgacc ttccgccgtc atgataaaac
     2461 tcactaaact gtcgatacgt acagtatagg atttttatac agtgcacgga agtggtgacg
     2521 gtgtaactct ttctcttaaa ttgacgatta atccgtcagt tgaatgcaaa ttcagataaa
     2581 gaaccagggg cagcgtgatc actgcccctg gtcattaacc gagaaggtct tttgcggtga
     2641 gttctgtcag tgatgagagt ttgacgtttg ctaatacaaa acgtggatca ttttgcgctt
     2701 ctggcgcagg aacgacgatg gaacgcatgc gggctgcttt agaggcgatc atgccattta
     2761 ccgaatcctc cagcgctacg caggtcagtg ggtcaacgcc cagttttgct gcgcagtcga
     2821 gatatacttg cggatgcggc ttgctgtaag gcagtttttc ggccgaggcg agggcatcga
     2881 aactgtcgcg taagtcaaac atggtcaaca ctttttccag catatgtagt ggtgacgcgg
     2941 aggccagtcc caccaataaa ccttgttctt tgcataacgc aacggcttcg cgcacgcctg
     3001 gtaataatgg acgtgtctct tcaaccagtg aaatggcacg ggcaataacc cgttctacta
     3061 cttcctgacg gcttggccca ttccacggtt gccgggcgta ccaaagatcg accaccatat
     3121 cgatgcgtaa acctaaggtg tccggcagct cgttacgacg ggagatatcc acccccaggc
     3181 ttgccatcac atccagttca gctcgatccc ataaaggttc tgagtcgata agtaatccat
     3241 ccatatcaaa aattgcagca agaatctgac gcggggttga cattacaacc tctcctttag
     3301 agcattatgt gtaaggtatt aagccgtgct tatgattaag cgcctatcat atcgccttta
     3361 aagaatgggc gaaaacagat atctacaggt aaactaagtg aaacgacgcg tcttaatcaa
     3421 ggagaactca tgacgtatca acaagctgga cgcattgctg ttttgaaaag gattttgggt
     3481 tgggtgattt ttatacctgc attgatctct acattgattt ctttgctgaa atttatgaat
     3541 acccgacagg aaaaccagga aggcattaat gcagtcatgc tcgactttac tcatgtcatg
     3601 atcgatatga tgcaggcgaa tacgcctttt ttgaatctat tctggtataa ctccccgacg
     3661 cccaatttta atggtggcgt gaacgttatg ttctgggtga ttttcatcct gatttttgtc
     3721 ggactggcgt tgcaggattc cggtgcccgg atgagtcgcc aggcgcgctt cttgcgggaa
     3781 ggtgttgaag atcaactcat tctggaaaaa gccaaagggg aagaggggct aacgcgtgaa
     3841 caaattgagt cccgtattgt tgttccgcac catactattt tcctgcagtt tttctcactg
     3901 tatatcctgc cggttatttg tattgctgca ggttatgtgt tcttttctct gcttgggttt
     3961 atttaatcaa aaggccgcac ggagcggcca ttatcttcaa cccttatcat gctgctaata
     4021 atctatccaa tgactgctga gcaataacca aatgctgacc gccaaataac cttgcacgat
     4081 ttaacagcgt gtagagttgg taaaccggtt gacgttcaag gaaatcggca ggtagcgggg
     4141 atactgactg atagccgtca tagatttgtg gcggctgttc agtatgcagc ggtaacatcg
     4201 ccaggtcgca ctctcggtca ccccagtagc aggccgggtc gaaaatgtac gggccatccg
     4261 gacccagcgc acagttgccg gaccataaat cgccgtgcaa cagagaaggc tgcggttgat
     4321 gtgaggcgag acgctgctga atatgctcga cgatggcatc gatattgccg aaagcgatcc
     4381 ctttttctgc tgccagttcc aactgccagc caatccgttg ttcagcaaaa aacgttgacc
     4441 agcgacgttg ccaggtgttg ggctgtggag ttgtggagag cgcgttatcg aaatcgagtc
     4501 caaattgtgg ttggtcgctc cattgatgta aacgcgcaat ttgctgacca agaatatttg
     4561 cgctatgcgc atccagcgga cgaggtggga gataatccat caccagaaaa ctgtagtcac
     4621 ggtcagcgcc aactgcccaa accttaggca cggtgacggt tttactacgc gacagtaact
     4681 ccagttggtc ggcttcggcg gtaaaaccgg gaagcagttc cctttcatca catttgacga
     4741 aaaagtcatg tcctgcatag cgcaaatgcc atgcggcatg gacttctccg ccaggcagtt
     4801 cattacgcag ttcgatttcg ccttcaccta actgctcgct caaaagacga ctgattgcct
     4861 gccacatgat gtctctccca tgttgtctgc cagatcataa ggttagcgca agaatgcggt
     4921 gtaaaaacac gagctaacgc acaccacaga ctgattattg cccaaaaacc atttattttc
     4981 tgttgctatt ccattcctcc caggtcgtga tggtgatttc aggatctttg ccggttgcac
     5041 tttgggtaag ccccgaaacc agttctctaa tttcttcttc actttgggta ctgataagtc
     5101 caaaagtgtt agtccccagc tcatggatat ttccctcatc atccgtcatg gtgagcaaaa
     5161 aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc agtcaatgta tcctcatgca
     5221 acatgacggt tatgacataa cggacaagat cgccactggc catagttcac ccctttgtta
     5281 tcacatgact tttgagcata gtcggagaaa cgcgttgccg acaggttggt gatgattccc
     5341 ccaatgctgg gggaatgttt ttgttagcgg gaaaggtaag cgtaaatttt ttgcgtatcg
     5401 tcatgggagc acagacgtgt tccctgattg agtgtggctg cactccccgc agctacgcca
     5461 aaacgaacca tctcttcaag agaggcattt tctgccagtt tcagtgtcat cgcgccgacc
     5521 atgctgtcac cagcgccaac ggtactcagg cttttcaccg gtggtggcac cacctgaatg
     5581 cagttttcac tatcaacacc cagcgctcct tgtggaccca gggaaacgac aacccgtttg
     5641 gccttgccgc tattaacgat ttcctgcgcg gctttgcgga catcgtctgg ctgggtgagt
     5701 tcgcgattca ccagcgcact gagttctttt tggttaggct taaccaactc gatgttacca
     5761 atcgccagtg ctgcacttaa cgcttcgcca gaactgtcga tgatgcagcg gatcccttgg
     5821 ttttgcgcag cggaaatcag ttgggttaat ttttccagct tcacacctgg cggcaggctt
     5881 ccgcttatga ccaggatggc cccggattca atttccagaa cttgctcttc aagctggcga
     5941 aactcatctt catttaatgc cgcgcctggc ataacaaaac gatactgctc accgcttgct
     6001 tccacatgta cgtgtaaatt ctgccgggtc cagtctttgg cttctacagt agcgacgggg
     6061 acattttcat ccgccaacag tgaaaccagg tgttcgccgg tcgcgccacc cgccgggaag
     6121 atcgctgtgg cactgcctcc aagatgggca atggcgcggg cgacgttgat gccgccgccc
     6181 ccgggttcga acaccggtgc ggtacagcgc agttttcctt cgggataaat ttgcggggta
     6241 attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat agatacgtac catcatttcc
     6301 tcctataggc tgatttcagt ctggcaccga atcagcggaa agtgaagaaa ttaacaatat
     6361 gatttattgg a
//
LOCUS       DAPGRN010000195         6338 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPGRN010000195 DAPGRN010000000
VERSION     DAPGRN010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.197"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            180..1325
                     /locus_tag="RHW33_004486"
     CDS             180..1325
                     /locus_tag="RHW33_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV1647918.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1419..1952
                     /locus_tag="RHW33_004487"
     CDS             1419..1952
                     /locus_tag="RHW33_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV1647919.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(1949..2266)
                     /gene="sugE"
                     /locus_tag="RHW33_004488"
     CDS             complement(1949..2266)
                     /gene="sugE"
                     /locus_tag="RHW33_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1647920.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3117..3575
                     /gene="traH"
                     /locus_tag="RHW33_004489"
     CDS             3117..3575
                     /gene="traH"
                     /locus_tag="RHW33_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraH"
                     /protein_id="HDV1647921.1"
                     /translation="MNRIIPAGCLAALFLAGCNSPSQHTPVPRNYGAASDVAVTRHTQ
                     YTLWDKGVFSGQLPVSTGSHLLTANSDRLSFDWEGDAIELLNELARVRGMQFNYNGVR
                     LPLPVNLHVRDMTFSNTLRLIEAQTAWRATIHQYPGLLQVSFMQPENRKK"
     gene            3572..4390
                     /gene="traI"
                     /locus_tag="RHW33_004490"
     CDS             3572..4390
                     /gene="traI"
                     /locus_tag="RHW33_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer lipoprotein TraI"
                     /protein_id="HDV1647922.1"
                     /translation="MKALTSLLLPVILLTGCSSSPTISPGGVNTPPPDINAWLKPRSE
                     KPDSLSETRWKMLTDAGKTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISPEG
                     WLPPVIDEAQDVAHITPDQIRTSSRVWTIIRPERFVSNPPGWRDWLLRGLSTTATPGT
                     EGSVVPEDSVQRKVWETALRQGWQEGRQNADLTLEANQKTLTRDYRGMMLYSLLWRQG
                     MITRPDVSDQMQTVTGDGKKLVTGDRVRRLKNHAEFNLQKSHWRPLIGTEGGSR"
     gene            4387..5535
                     /gene="traJ"
                     /locus_tag="RHW33_004491"
     CDS             4387..5535
                     /gene="traJ"
                     /locus_tag="RHW33_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HDV1647923.1"
                     /translation="MNIPDEFGLFPFQRFTADELRHFFVWCAAHKVSDVDLTGGSPVS
                     VSRFGRRVRCSSATLPTTLMSSLIDELFGREVIPRVLAGNPVDRTIQINGDASGRYGL
                     KRGERIRLRSHLIQGTSGAEEKAISITMRVIPTEIPDILSMNIEPDLLEAMVCKSGLG
                     FVCGETGSGKSTLCSALYRYIMDNFPDAKIVTYEDPVEYILGNENDLLPPHQAEIGRD
                     VVSFAAGLRSAVRRNPEIIGVGEIRDNETADAAVQAGNTGHYCLSTMHTKSPGETLAR
                     LLGLFPPVIRDSMAWAVLSLLQFILVQVLVRTNDGGRKAVREYIVINDELRDNLSGMP
                     HAEWGHHIDAIIRQEKRRIRDQILEMYIRNEVDRREAILFIPPGELRS"
     gene            5532..5822
                     /locus_tag="RHW33_004492"
     CDS             5532..5822
                     /locus_tag="RHW33_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDV1647924.1"
                     /translation="MKREGFWRYATPPVNIQGVPLPFTMIYLFICPFPSKTTFWICTG
                     IILFFVILDRSGWTVRTIYTRIFSILRGAIASGRPWWYRHHTESPRDWTGLD"
     gene            5837..>6338
                     /locus_tag="RHW33_004493"
     CDS             5837..>6338
                     /locus_tag="RHW33_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HDV1647925.1"
                     /translation="MSGKKTIVTLLRVSLLACPLLFTTPSFAMIDTPSVKVGFSPEGS
                     ASALVLDTINSAESSIRMMAYSFTDPDVMHALAKAKKRGVDVRIVVDDKGNTNRASQE
                     AMKYINLLDIPLRTVDAFPIHHDKVIIVDGNTVETGSYNFSRAAARKNSENVVVLKNM
                     PDVAAQY"
BASE COUNT         1621 a         1620 c         1576 g         1521 t
ORIGIN      
        1 agtctaaatt cttcgtgaaa tagtgatttt tgaagctaat aaaaaacaca cgtggaattt
       61 aggaaaaact tatatctgct gctaaattta accgtttgtc aacacggtgc aaatcaaaca
      121 cactgattgc gtctgacggg cccggacacc tttttgcttt taattacgga actgatttca
      181 tgatgaaaaa atcgttatgc tgcgctctgc tgctgacagc ctctttctcc acatttgctg
      241 ccgcaaaaac agaacaacag attgccgata tcgttaatcg caccatcacc ccgttgatgc
      301 aggagcaggc tattccgggt atggccgttg ccgttatcta ccagggaaaa ccctattatt
      361 tcacctgggg taaagccgat atcgccaata accacccagt cacgcagcaa acgctgtttg
      421 agctaggatc ggttagtaag acgtttaacg gcgtgttggg cggcgatgct atcgcccgcg
      481 gcgaaattaa gctcagcgat ccggtcacga aatactggcc agaactgaca ggcaaacagt
      541 ggcagggtat ccgcctgctg cacttagcca cctatacggc aggcggccta ccgctgcaga
      601 tccccgatga cgttagggat aaagccgcat tactgcattt ttatcaaaac tggcagccgc
      661 aatggactcc gggcgctaag cgactttacg ctaactccag cattggtctg tttggcgcgc
      721 tggcggtgaa accctcagga atgagttacg aagaggcaat gaccagacgc gtcctgcaac
      781 cattaaaact ggcgcatacc tggattacgg ttccgcagaa cgaacaaaaa gattatgcct
      841 ggggctatcg cgaagggaag cccgtacacg tttctccggg acaacttgac gccgaagcct
      901 atggcgtgaa atccagcgtt attgatatgg cccgctgggt tcaggccaac atggatgcca
      961 gccacgttca ggagaaaacg ctccagcagg gcattgcgct tgcgcagtct cgctactggc
     1021 gtattggcga tatgtaccag ggattaggct gggagatgct gaactggccg ctgaaagctg
     1081 attcgatcat caacggcagc gacagcaaag tggcattggc agcgcttccc gccgttgagg
     1141 taaacccgcc cgcccccgca gtgaaagcct catgggtgca taaaacgggc tccactggtg
     1201 gatttggcag ctacgtagcc ttcgttccag aaaaaaacct tggcatcgtg atgctggcaa
     1261 acaaaagcta tcctaaccct gtccgtgtcg aggcggcctg gcgcattctt gaaaagctgc
     1321 aataactgac gatgaggccc aggatattgg gcctcctttc tttctctttt tttcctgttg
     1381 tcatctacac ttaacaaaaa tacagcaagg aaaatcccat gcgcattttg cccgtcgttg
     1441 ctgcagttac ggctgcattc ctggttgtcg cgtgtagctc cccgacaccg ccgaaaggcg
     1501 ttaccgtggt aaataacttt gatgccaaac gctatctggg aacctggtat gaaattgcgc
     1561 gcttcgacca tcgtttcgag cgcggattgg ataaagtgac cgcaacatac agcttgcgcg
     1621 acgacggcgg catcaacgtt attaacaagg gctataaccc tgacagggag atgtggcaga
     1681 aaacggaagg gaaagcctat ttcaccggcg acccaagcag agccgcgctt aaggtttctt
     1741 ttttcggccc cttctatggc gggtataacg taattgcact cgaccgggaa tatcgtcacg
     1801 cgctggtttg tggtccggat cgcgactacc tgtggatcct ttcacggacc cctactattt
     1861 cagatgaaat gaaacagcaa atgttagcca tcgcgacccg ggaagggttt gaagtgaata
     1921 aactgatttg ggtgaaacag cctggcgctt agtgagtgct cagcttcaga ccaataatgc
     1981 cagcaacgat cagcccaagg ctcagcaaac gtgccgggct ggcagactca cccagcagca
     2041 aaatccctgt aatggccgcc ccaacagcgc caataccggt ccagaccgca taagcggttc
     2101 ctacaggcaa cgtgcgcatt gcccaagaga gcatggcgat actgacgatc atcgccgcaa
     2161 tagtgataat gcttggcgta agacgcgtaa aaccgtgggt gtatttcagg ccaatcgccc
     2221 agacaacttc gagcaaacct gcaattaata aaacgatcca ggacatatca ggctccagaa
     2281 caatggggcc gtccccggtg aaagaagcgt ttgcaggtcg tcctgcaaag ctaatgtgtg
     2341 aaatggcatt tttgcccgga agaaaatgaa tttcaacctt tttattcacc gcctgctaaa
     2401 agcaagaatt aagcataatt agcggcgtag ttcccgcatt tggcactcat cggagttctc
     2461 tatgatgaag ggctttctga aaccagaaag tcgattgccc ctcttctgaa tgcgaaacgt
     2521 ttatgcttaa aattttagtg attgaccggt gccactttac ccgcacaggg atagagtaat
     2581 aatgacacat aatcattgtt tatcatttct ggccagagat aggctcggca agaaattcaa
     2641 accggcgtac cttgatgctc tgatcatgca ttacgatggt acccggctca tacttgacgg
     2701 aaaatacaac aaagaggcat agaggatatt tgaatttaca taaattctga tatactcagt
     2761 aagatacaag agtgtacggg cacatgtgcc tgtctgaatt gcaggttatc cggacgaggg
     2821 ccgggaactg tttcagggac aagcgccggt acgttcgatt cataaatcgt cgagtcatcc
     2881 atcatggatc gattcggcgc ggagcgttaa agattacgct catattcgtt ttatgtctga
     2941 cgacacatca accagccagt atcaggaata atcaccttca gtaaactatg aggctattta
     3001 cctggccaca ctggcattta tttccggtaa cgcaagtata gccggaaaca aacatcatcc
     3061 tgtctgtacc ggagcaccgg tatatcccat tctgatttaa ctgctcagga attattatga
     3121 accgtattat acctgcgggg tgtcttgccg ccctttttct ggctggctgc aactcccctt
     3181 ctcagcatac tcctgttccg cgtaactacg gtgctgcctc tgatgtggcg gttacccggc
     3241 acactcagta cacattatgg gacaaaggtg tattttccgg ccaacttccg gtttcaacgg
     3301 ggtctcatct tctgacagct aacagcgatc gcctttcctt tgactgggaa ggtgatgcaa
     3361 ttgaattact gaacgaactg gccagagtca gggggatgca gtttaactac aatggtgtcc
     3421 gcctgccgtt accagtcaac cttcacgtcc gggatatgac gttttccaat acgttgagac
     3481 ttattgaagc ccagacagcc tggagagcaa caatccatca atatccgggc ttgttacagg
     3541 tcagcttcat gcagccggag aaccggaaaa aatgaaagca ctcacctctc tccttttgcc
     3601 agtaatactg cttactggtt gttcttcttc ccctaccatc tcgccaggtg gtgtcaatac
     3661 gccaccacct gacattaatg catggcttaa accacgaagt gaaaagccgg atagtctgtc
     3721 agaaacacgc tggaaaatgc tgacagacgc aggtaaaacg cttggatttc gtggtggcaa
     3781 ggctcagcgt tcatgggagc tgattcaggc gctcaacgcc cgggaaagca cacttaatgc
     3841 cttatacgat ttccggccac tgattagccc ggaaggctgg ctaccaccgg ttattgatga
     3901 agcacaggac gtagctcata tcacgccaga tcaaatccgg acctcttccc gggtctggac
     3961 catcattcgc cctgaacgct ttgtcagcaa tccaccgggc tggcgtgact ggcttctccg
     4021 ggggctttca acaacagcca ctccgggtac agaaggcagc gtcgttccag aagacagcgt
     4081 gcaacgaaaa gtgtgggaaa ccgcattacg gcagggatgg caggaaggaa gacaaaacgc
     4141 cgacctgacg cttgaagcca accagaaaac cctcacccgt gattaccggg gcatgatgct
     4201 gtactcactt ctctggcgtc agggcatgat cacccgtccg gatgtttctg atcaaatgca
     4261 aaccgttaca ggtgacggga aaaaactggt cactggagat cgtgttcgtc gccttaaaaa
     4321 tcatgcggaa ttcaatcttc agaaatctca ctggcgtccg cttataggca ctgaaggagg
     4381 ctcccgatga atattcctga tgagtttggt ctttttccgt ttcagcgttt cactgcagat
     4441 gaactacggc atttctttgt ctggtgtgcc gcacataaag tcagtgatgt ggatctgacc
     4501 ggcgggtcac ctgtatctgt cagtcgcttt ggccgacggg tgcgttgttc cagtgcaacg
     4561 ttaccaacca cactcatgtc ctccctgatt gatgaactgt tcggtcggga agtcattccc
     4621 cgcgttctgg caggcaatcc ggttgacagg acgatacaga ttaacgggga tgcatccggg
     4681 cgctatggtc ttaaacgcgg agaacgcatc cggttacgct ctcacctcat tcagggcaca
     4741 tctggtgcgg aggagaaagc aatctcaata accatgcgtg tcatcccgac agaaataccg
     4801 gacattctct ccatgaacat tgaaccagac ctgcttgaag caatggtctg caaaagcggt
     4861 ctgggatttg tatgtggtga aaccgggtca ggaaaatcaa cgctgtgttc agcactgtac
     4921 cgttacatta tggacaattt ccccgacgcc aaaatcgtga catatgaaga cccggtggaa
     4981 tatatcctgg gaaatgaaaa cgatctgctc ccaccgcatc aggcagaaat cgggcgtgat
     5041 gtggtcagtt ttgctgccgg actgcgttct gctgtacgac gtaatcccga aattatcggt
     5101 gttggtgaga tcagggataa cgaaaccgcc gacgcagcgg tacaggcagg caacaccggg
     5161 cattactgcc tgagcaccat gcatacgaaa tctcctggtg aaacgctggc acgtcttctt
     5221 gggctgtttc cccctgtcat cagggattcg atggcctggg ctgtcctgtc actgttgcag
     5281 ttcattctgg ttcaggttct ggtcagaaca aatgacggtg gtcgtaaagc cgtccgggaa
     5341 tatatcgtca ttaacgatga gcttcgcgac aatctttccg gaatgccgca tgcagagtgg
     5401 gggcatcaca ttgatgccat aatccggcag gagaagcgcc gtatccggga ccagattctg
     5461 gaaatgtata tccggaatga agttgaccga agagaagcga ttctttttat cccgccaggg
     5521 gagttacggt catgaaaaga gaaggtttct ggcgatatgc aacaccgcca gtcaacatcc
     5581 agggcgtacc tctgccgttc accatgattt atctgtttat atgccccttt ccctccaaaa
     5641 caaccttctg gatttgcacc ggcatcattt tatttttcgt tattctcgac cgttcaggct
     5701 ggactgtcag gactatctat acacgcattt tcagcattct gagaggggcc atcgcctccg
     5761 gtcgtccatg gtggtatcgc caccatacgg aatcaccacg agactggact ggcctcgatt
     5821 aaattctgga gtctttatgt caggtaaaaa aactatcgtt actctgctcc gggtatcgct
     5881 actggcatgc ccgttactgt tcacgacacc atcgtttgca atgatcgaca cgccatccgt
     5941 gaaggtcggc ttttctcctg aaggaagcgc atccgctctg gtgcttgaca caatcaacag
     6001 tgcggaaagt tccatcagga tgatggcata ttccttcacc gacccggatg taatgcatgc
     6061 gctggcaaaa gcgaaaaaac gcggagtgga cgtccgtatt gttgttgatg acaaggggaa
     6121 tacgaaccgg gcaagtcagg aagcgatgaa atatatcaac ctgctggaca ttcctctccg
     6181 gactgtagat gccttcccca tccatcacga caaagtcatc attgttgacg gaaacacggt
     6241 tgaaactggc tcctataact tctctcgtgc tgctgcacgc aagaattcag agaatgtcgt
     6301 tgtgctcaag aatatgccgg atgtagccgc acaatatc
//
LOCUS       DAPGRN010000196         6260 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPGRN010000196 DAPGRN010000000
VERSION     DAPGRN010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.198"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            156..881
                     /locus_tag="RHW33_004494"
     CDS             156..881
                     /locus_tag="RHW33_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647926.1"
                     /translation="MKGEVKERGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMIRKIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNDISECDARDEGVSPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWSANPWVWVIEFERIQ"
     gene            921..1112
                     /locus_tag="RHW33_004495"
     CDS             921..1112
                     /locus_tag="RHW33_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HDV1647927.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKVEKAEGATK"
     gene            1109..1324
                     /locus_tag="RHW33_004496"
     CDS             1109..1324
                     /locus_tag="RHW33_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647928.1"
                     /translation="MSKPTDEEIVRVLEEHGRCMTYVVTNWLRDKYRTLKTAYVLRRL
                     KKLEFDGKVKRVNSSYIRQICWEATSE"
     gene            1317..1961
                     /locus_tag="RHW33_004497"
     CDS             1317..1961
                     /locus_tag="RHW33_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647929.1"
                     /translation="MSNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTEYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKEQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            1958..2650
                     /locus_tag="RHW33_004498"
     CDS             1958..2650
                     /locus_tag="RHW33_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV1647930.1"
                     /translation="MTIDYQVLREAAEKATPDEWVAFISTDTGTYAVHTPGDERCEDV
                     IKWTGFDGQKNAENNARHVAAFNPKVALELLGEIKCLEDTNIDATCRISELETNLAAL
                     VAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIINDALHHSETPATDAFLAEVRTEARK
                     EGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPEGDFDRSFSDGVLE
                     DIAAQLRKGGNQ"
     gene            2647..3246
                     /locus_tag="RHW33_004499"
     CDS             2647..3246
                     /locus_tag="RHW33_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HDV1647931.1"
                     /translation="MSEINYQALREAAQNAKDLGGIKNYKRGEQAVAEFESLITPHIV
                     LALLDERERNQQYIKRRDQENEDIALTVGKLLIENGQLVADTLRHLADNEIDSDYFAI
                     TSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAEKRIAELSASHSKLRDTMAGI
                     HNTIRMDGGYTPLAAILNAAKRAYEESASAAGIRIKGGE"
     gene            3246..3422
                     /locus_tag="RHW33_004500"
     CDS             3246..3422
                     /locus_tag="RHW33_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647932.1"
                     /translation="MRVACIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARREAKKRRRAKR"
     gene            3419..3607
                     /locus_tag="RHW33_004501"
     CDS             3419..3607
                     /locus_tag="RHW33_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647933.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            3609..4124
                     /locus_tag="RHW33_004502"
     CDS             3609..4124
                     /locus_tag="RHW33_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647934.1"
                     /translation="MALTHRELCQIAYKFLKRNGFKVCFHDRFIAVTSTGEQPDAMGF
                     RNSASCLIEAKCSRADLLADRKKRFRKNPSLGMGDWRFFISEPGIISIEDLPPGWGLL
                     HVVNGRVRKVHGWPKGNCCWGNPDDKPFTGNKQVECDYMLSALRRMELRGHLNEIYDG
                     VIVNKKEGNAA"
     gene            4946..5419
                     /locus_tag="RHW33_004503"
     CDS             4946..5419
                     /locus_tag="RHW33_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647935.1"
                     /translation="MDFGQWLEQQRGKIDVDCGCVSTETFMHWLRVAYEAGNYPDIPD
                     SSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTQQLRERISRVLGDVITPYH
                     HKQAEKSGLERCHLEAALNIKPGHTLGIIDALLVHKMARALLSLVDAGDTSEGEV"
     gene            5416..5778
                     /locus_tag="RHW33_004504"
     CDS             5416..5778
                     /locus_tag="RHW33_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647936.1"
                     /translation="MRVADHIKHLERIIENGELLRDQMRRTAEAREAIIRSQAGKLKQ
                     LSEINTLYKNRCNRAVMRLQKARNEIKLVEAKLKKQIQRYDQQDAFYAAIKAAANEIG
                     IWKLLVEKAKTKLNANES"
BASE COUNT         1805 a         1310 c         1688 g         1457 t
ORIGIN      
        1 aaaataaaaa catgccgcaa ggcgcggcat gtatccaatc aatcacagga gctgaaaata
       61 tgaacacggc attcaaaatc attatggccg cgatctattt ctggctgttc tctatcactt
      121 ttggcggcat cgtcgcgcat gggtaagggg gatgcatgaa aggcgaagtg aaagagcgcg
      181 gcatgatttt caacgatgag atggtccgag ccatcctcga aggaaggaaa acgcagacgc
      241 ggcggataat gaaaaatcag cctgcggaag ttggtccaga agcaccagtg atgattagaa
      301 aaattggtgc aggttttcag tggtacgggg ctgatggtgt aagcagtgtt ttcaattgcc
      361 cctttggtat cgtcggcgat cgaatttggg ttcgtgaaac atgggcgata ttaggcaatg
      421 aagatggttg cagtgtggac tggaacgaca acctttgtcg tggcgatgag aagaacgcag
      481 caaggattta tcgggccagt tgtgagcaaa agcctggtga ttacggttta tggtcgatac
      541 ccgatgatgc cgactggaaa cctcacactg tgaatgaaaa gtttgatggt gggtggcgtc
      601 catcaattca catgccgcga tgggcatcac gcatcctgtt ggaaattacc aacgttcgcg
      661 ttgagcggtt gaacgatatc agcgaatgcg atgcaaggga tgagggcgtt tcgcctgctg
      721 gaagtttgct tcctgatcac ccgggaacat tccttactcc caagggggat ttcgcaatgg
      781 ccaaggttgc gttccagcgc ctgtgggaat ccatctacgg cgaagaaagc tggagtgcta
      841 atccctgggt ttgggtaatc gagtttgagc gtattcagta gggcgagcgt atgcaatcag
      901 ttatttgtga ggaagtcggc ctgaataaag catccccagc agagttaaga gcaagtctcg
      961 aaatggcgca tagccttgct caaattggtg tcaggtttgt accaattcca gttgaaacag
     1021 atgaagaatt tcacaggtta gcggcatcgg cagcacaaaa gctggaaatc atggcagcga
     1081 aagtagagaa agctgaagga gcgacaaaat gagcaagcca accgacgaag aaatagttcg
     1141 ggtgttggaa gaacacgggc gctgtatgac ttacgtcgtg accaactggc ttagggataa
     1201 atatcgcaca ctcaaaacgg catacgtttt gcgtcgtcta aagaaactgg agttcgacgg
     1261 gaaagtaaag cgcgttaaca gttcttatat aagacaaatt tgctgggagg caaccagtga
     1321 gtaaccgttt ttacatgatg tgcttgcgtg aaactgtggg taataacgcc tcattccatt
     1381 gccataacgg caatggttac agctctgata tcgatcgcgc tcatgtttac acgctggaag
     1441 aagcccaaaa agcctggaat tgtggtcgag atatcgatca gcctgtttgc gctgatagcg
     1501 tggatgcaat ggcagtgtgg cacgttgatt gccagtacat ccctacagaa agcctgattg
     1561 agtcagattg cactgagtat gtggcctaca aaaaaggtag ctggaacggc aacgatgttt
     1621 actggcttca acacggtgga ttgccaacag atgacttcag taaagcgacc atctttagcg
     1681 tcgccaacaa aaacgaacca ggaatagttt ggttgccatt ttccattgct gatgcagcaa
     1741 agcgccggac gttcaatatc aataacttta accgcagaac aatggttcag ggcgcaggtt
     1801 tggtcatgcc tgactggttg aaagagcaga acagaagaaa gaagtcgcga agcgggaagg
     1861 tgcgttggaa ttgtccgcat tgcggaaaaa taacctggca gtacagccca tatgattttg
     1921 aaggctgtag tgattacaac tgtgaaggat ggcgagaatg acaattgact atcaggtact
     1981 gcgtgaggcg gcagaaaagg caacaccaga cgaatgggtc gcatttattt cgacggatac
     2041 tggtacttat gcggtgcaca cgcccggtga tgaacgatgt gaagacgtta tcaaatggac
     2101 aggctttgat ggacagaaaa atgcagagaa caacgctcgt catgttgccg cgttcaaccc
     2161 aaaggttgca ctggagctgc ttggtgaaat taagtgcttg gaggacacaa atattgatgc
     2221 cacgtgccga atttcagagc ttgagactaa tctcgctgcg ctggtggcag agaacgctgg
     2281 gctgaaacac gcaatggccg taactcttga gcatgtgtcg gtcacggatg cagggcaggc
     2341 cggagttgct gcaatgatta tcaacgatgc cctgcaccac agcgaaactc cagcaaccga
     2401 tgcttttctg gctgaagtga ggactgaagc acgcaaggaa ggcgcttatt ttgtggcgaa
     2461 cagaatgctg gctgcctggg aagctggttt tattgatgat actgcgaaga acgccgcgga
     2521 tattgcccgg atgattctta cctctactga gtttatggct aatgcgccgg aaggcgattt
     2581 tgaccgctca ttctctgatg gcgttctcga agatatcgcc gcccagcttc gaaaaggagg
     2641 caaccagtga gcgagattaa ctatcaggca ctgcgtgagg cggcgcagaa cgcgaaagat
     2701 ttaggtggga ttaagaatta caagcgaggc gagcaagctg ttgccgaatt tgagtccctg
     2761 ataacgccac acattgtgct ggcgcttctg gatgaacggg aaagaaacca gcaatacatc
     2821 aaacgccgtg accaggagaa cgaggatatt gcgctaacgg tagggaagct gttaatcgaa
     2881 aacggtcagc ttgttgccga tacgctacgc cacttagctg ataacgaaat cgactctgat
     2941 tattttgcta tcacctcaac gaatgagaac ggtactgaaa ttgatcatga gatggctatt
     3001 accgattacg cactgcaagc tgccggaact gtagacgaat tggttgcagc gctggaatcc
     3061 gcagagaagc gcatagcaga actgtctgct agccacagca aattgcgcga cacaatggct
     3121 ggcatccaca acacaatccg aatggatggc ggctatacgc cactggcagc aatccttaac
     3181 gctgctaaac gcgcatatga agaatcagca agcgcagctg gcattcgcat caaaggaggt
     3241 gagtaatgcg tgtggcatgt atcggcttgt taccgtaccc gactcgtttt tgggcttctg
     3301 cgctaattgc gaagccgcat gtcctgatgg ctgacaacat catcccggca ccaaagcgcc
     3361 gccataccgg tattgcggct gcacgacgcg aagcaaagaa acgcaggaga gcaaaacgat
     3421 gaaaaaccgt aaagcaaaga ttctgttagt tcgtagaaac gctcctggcg tctggcagtg
     3481 ggtgagactc agcaaccgac ggatggggtt gatgaaatat tacgggatga tggattgtgg
     3541 tttttgcaaa aagcccagcg cggcgcaaaa ccgctggaaa aaccacttgc gcactaaagg
     3601 agagtgatat ggcgttaaca caccgcgaac tctgtcagat tgcgtataag ttccttaagc
     3661 gcaacgggtt caaggtttgt tttcatgacc gctttatagc tgtaaccagt accggagaac
     3721 agccagatgc tatgggattc agaaattcag catcatgcct gatagaagcg aagtgttctc
     3781 gtgctgactt gttagcagat agaaaaaagc gttttcgtaa aaatccgtct cttggaatgg
     3841 gcgactggcg attctttatt agtgagccgg gaattatttc aattgaggat ttaccacctg
     3901 gctggggatt acttcacgtt gttaacggaa gagtacggaa agtacatggg tggcccaagg
     3961 gtaattgctg ttggggtaat cctgacgata agccatttac tggaaataag caggttgaat
     4021 gcgattacat gttatctgca ttaaggcgca tggagttgag agggcacctt aatgaaatat
     4081 atgacggtgt aattgttaat aagaaagaag gaaacgcggc atgatcacta ttaccaaaga
     4141 gcgactactg acaatcaagc agtggcgcga aacatacgga cctggtagca acgttgtact
     4201 gccagcagaa gaagcggaag aactggcgcg aattgctctg gcatcgctgg aagcagaacc
     4261 agttgcttat attttcaaac atccggccgg gaaattattc tgggctttaa cggatgaaag
     4321 caataaagag caaccggacg ttattcctgt ttatgctgac tcacctgcgc cggttgtgcc
     4381 ggatgaaata gacgtaaacg atgtaccaag ggcggtgaca tatttcaaga cacacagaga
     4441 ttgttacgca gatggctgga acgcctgccg cgccgccatg cttcagtccg gaaactttcg
     4501 ggaaaacaag aattcgtcaa ccaataattt tcgggaaatc gcggaaacgt caaccaacta
     4561 tccggtaatt cctagtgagg tgttgtccgc aatcctgaag gttgccaaga ttcgtgccga
     4621 tttcgatgat tttgacggtg acaggcgagg tatcggtgat tgtctggatg aggctgagca
     4681 agagcttata gttaccatta acaaatatgc cagtcagttg gcagcagaac cgatagcgac
     4741 taatgacgtt cgagagcaaa cagccgttcc gccagttcct gtaatacagg ctgatgtcgc
     4801 gcaggcaatt gaaaaactca aacggaaatt agtggaatgc aatcgctata actactgcgc
     4861 agatgcagtt aagggcgttg agtatgcctg ccacgctgcc atgcttcagg gtagccaacc
     4921 tgtaagccaa agtaagactt acaggttgga ttttgggcaa tggctggaac agcaacgcgg
     4981 gaaaatcgat gtggactgtg gttgtgtgtc cactgaaaca ttcatgcact ggctgcgggt
     5041 agcttacgag gctggcaact atccggatat tccggatagt tcggtgccag cgccaggaaa
     5101 gggcgtcacc ggtgaacgta tccgcattaa gccgcatgtt tatcgcgaac tggttaaccg
     5161 tctccacgat acagcgatca agtgtgctgg cacccagcaa ttacgagaaa gaattagccg
     5221 tgttttgggc gacgttatta cgccatatca tcataaacaa gccgagaaaa gcggtctgga
     5281 aaggtgtcat cttgaggcag cattaaacat taagccgggg catacgcttg gcattattga
     5341 tgcactgttg gttcataaga tggccagggc tttattgtcg ttggtggacg ctggtgatac
     5401 aagcgagggt gaagtatgag agttgcagat cacatcaaac accttgaaag aattatcgaa
     5461 aacggtgaac tcttaagaga tcagatgaga cgcacggcag aagccaggga ggcgataatc
     5521 cgcagtcagg ctggtaaatt aaagcaattg tcagagatta acacgctata caagaacaga
     5581 tgtaaccggg cggtgatgcg gcttcagaaa gcacgtaatg aaattaaatt ggtggaggca
     5641 aaactgaaaa aacagatcca gcgttacgat cagcaagatg ctttttatgc cgccatcaag
     5701 gcggctgcta atgaaatagg catctggaag ttgctggtgg agaaagcaaa gacgaagtta
     5761 aatgccaacg aaagctgaac tacaggtgcc caccagcaca tacagaaaat gattgtttcc
     5821 acatcaagga gattttaatg tttcactgaa acattaagta agccagtgca taattccatt
     5881 ttttactgac cttaaaagca aaatcaaaac gatgatgagg ataatagcca gaatctggct
     5941 aataataggc gcatctaaaa atgcactcag gaactgaaaa aaagcgttca ttcaggtggt
     6001 tccttgtcaa atgtaaaggt gcacttgctc acgttgacgt agaaacccaa cccctatata
     6061 gttggattcg gtgaatgaaa gtcgttaacg tgagcttacg gcacatgttt tcggaaaaac
     6121 atcagggaac ggctaattcc ttgatgcggg tggggtctgt aatgcagacc ctatctatta
     6181 acgtcatgat tgcatctcaa attttctcct tatcttcaat taatctacat tcatttcatc
     6241 tgttagccac cacaatatgt
//
LOCUS       DAPGRN010000197         6170 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPGRN010000197 DAPGRN010000000
VERSION     DAPGRN010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.199"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(406..1230)
                     /locus_tag="RHW33_004505"
     CDS             complement(406..1230)
                     /locus_tag="RHW33_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647937.1"
                     /translation="MNRSNEEDIEKAKSLISIGAEIAGAAVGGAIGFFATSPVGAAGA
                     GSLGVIVAKAGAKLLGDVADRTMSAREKVRVGAAAAVAFNKIEKVALSGNEPRKDDFF
                     NSEEGKRSDAEEILEGTLRRAREEYEEKKILLLGNFYANMVYSPGVSTEEANYYLRLF
                     DSLTYRQLCVMSLIMMKPQYSDFQNLRSHDYRTPNANMQASTVALLQEIYALYNLGLV
                     CVRSAGGNGYVSLLAWHDIIPLGLEFTGLGERFYKLFMGGGSLSLPDITTIAEALR"
     gene            complement(1915..2850)
                     /locus_tag="RHW33_004506"
     CDS             complement(1915..2850)
                     /locus_tag="RHW33_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647938.1"
                     /translation="MSFLKTLMDSLSAISGSVANASETEKKKDRRSDEEFTDFKDDSS
                     LINYLTKQYIDNELAVDKSKNHWEVFSQHLKPLIHCLKRYEDASLSQRETSQKEQAAA
                     MLEALQQGEELSEHSGNGTIIGRDDYVNGLKKITLSYCQKHVLTMVDFLDNVPDHLCD
                     GKNASTSWQSFRKDASKKRWKNFDQACRDNSLYFSVGKVLFDTTLWGSGKHGIMLGRD
                     DITCLGGEPDKFRVLWSNVTSLWHHKGYLYVNDYKTGFVANDDACKLLELLEEHFDKM
                     KRSEGNLLLAYLGYDRSSQQAIKNAYEADVQQFIE"
     gene            complement(2847..5159)
                     /locus_tag="RHW33_004507"
     CDS             complement(2847..5159)
                     /locus_tag="RHW33_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1647939.1"
                     /translation="MNDKLKMWRDKAGLSQQEVAERAGENVLSQKQVSRIEQAPLSQN
                     LELVLAYLRIVAPEKIQEFITDCLKTQLNTSSLNQNYTQQKENMMRNKKELIEYLERS
                     IATLSNTSNPFIKNSSITKDIAELKDNLKDIHAKPVLSAMGPSDAGKSTLLNYILGSE
                     ILPAKWQPATCLVTLLMHTQDKPDYLDKNKSVFIFKNGFKPHMIFDRQMLADFLIDSG
                     GKELIKTYGEKDENDNVLNPQAYMVVVFESFAILETIWLLDTPGQLIEPEMYDEIDGC
                     SSEEILNDKAKAMSAISLVDGLIFASATTKFMRDEDPEFLQNILMHKVPLEGDKPLAN
                     LIILKTHSYDQITAEANHKTFRTAANHLTKTFSHTLYDEWEHQLNNPNHPQKLLRPDA
                     AVWASRMLPFFRENRQYCEAFDVRLKEVINYLLANRTALINNRIETIHKRIQNRIALA
                     LQELQQSSRSVQERKEDIEQQDARFREGSGALIARFTEISNFCPRYAEEDIERIRDIF
                     YSLCNDESMYRFIDNRFEDKDEAKRAINKALGNHFESKFRRVMEESTRRFSLEVESVV
                     QAWQKIVPSAQGAARAGMPETDISLSATSDFDAYSAFVGGMAGLTSFGAMAGYVATIS
                     SNLGAYILVGKAAGVLTSLGITSSVTTLPTLVAATGGPVAWGVAIAAVVGLLVYRLFA
                     NWKKALAKSVVKGLESSNTLSNVEADITRYWQDSRNALRASVDSLKNETDAFIRHLYA
                     EANIEYQQQDIAEAEQILNTIKNNLGSEDK"
     gene            complement(5464..5700)
                     /locus_tag="RHW33_004508"
                     /pseudo
     CDS             complement(5464..5700)
                     /locus_tag="RHW33_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5716..6170)
                     /locus_tag="RHW33_004509"
                     /pseudo
     CDS             complement(5716..6170)
                     /locus_tag="RHW33_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1586 a         1458 c         1300 g         1826 t
ORIGIN      
        1 tagtaataaa cctaaattaa aattatccat gtttgatgag tgaaaataat gttaggaaat
       61 gaacctgttt aaaaactcgt tatcatagat taaccgttca gatgctcaac gaactcaata
      121 aattggctga gtctggtgta ttaactcaga cctgatctga cagttaccgg ttatgtaaac
      181 atttatcgtt gctgccaatg cttggattat tgtcctcaag gtattcgctt tacttacaag
      241 ctaattttga aacagatgtc gcgctatttt aggaagagaa tctatgcaaa agtttgctat
      301 gagcgaggag cggatgttta tgattgttat caacgaggcc ccgtgtctaa caatctcctg
      361 tgttaataag caggggctgg aaactttgaa tcaaaatgtt cattcttaac gcaaagcttc
      421 tgcgatagta gtaatatcgg gaagtgataa gcttcctccc cccataaaaa gtttgtaaaa
      481 cctttcacct aaccctgtaa attctaatcc taaagggatg atatcatgcc atgccagcaa
      541 agatacataa ccatttccgc cagcactcct aacgcagact aatcccaaat tatacagagc
      601 ataaatttct tggaggagtg ctacagtact tgcttgcata ttggcatttg gggttcgata
      661 gtcatggctt ctcagatttt gaaagtcaga atattgtggt ttcatcatga tcaaactcat
      721 aacacagagc tgccgatacg tcagtgaatc gaacaaccgg agatagtaat tcgcttcttc
      781 tgtactcact cccggagagt agaccatatt tgcataaaaa ttccctagaa gaagaatctt
      841 cttttcctca tactcttcac gcgcccttct taacgtcccc tccagaattt cttctgcatc
      901 actgcgtttt ccttcttcag agttaaagaa atcatctttt ctgggttcat tacctgacag
      961 agcgaccttt tcaattttat tgaatgcaac tgcagcagca gcaccaacgc ggactttttc
     1021 gcgtgcagac atcgtacgat cagccacatc accgagaagt tttgcaccag ctttcgcgac
     1081 aatcacccct aacgaaccag ccccggcagc ccccacaggg ctagttgcaa agaagccaat
     1141 tgcaccgcca actgcagcgc cagctatctc tgcacctatc gaaatcagcg atttggcttt
     1201 ttcaatatct tcttcgtttg atctattcat agctttatct tctaggacag aataatggat
     1261 ggttgtaatg aagatacctt taccacatga aaatcattaa ctccacatta cctgatgaca
     1321 gtgaactgtt gtccgttgat taacacgcaa taatgctatt caaatggatg tttgaccacc
     1381 tggaactaac cgctgtaatg acatctattt ttggtacaga ggagaccaac cggcgtgtcc
     1441 tcaggtccga tatgagcgat aagcggaagt tcaaaatact tgttattggc ttttcgaaga
     1501 tcttgctgta gtaatcaaaa aggagcaggt agtgttatta acgggatagc tcccgaggat
     1561 attatgcatc atcacggcag cctgctttca tattttttaa tcttaataaa atttatctgc
     1621 gtattaaact accggatttt aggttttcca aaaatgatag tattaccagt ggactcacaa
     1681 ccttggcatt cctgattcta cttaaacagc cgtatccttt tgcgcacata ttcgagcgcc
     1741 agtgtgacga gttttgatca cgcattcctc tgctcactgt tttaacatta acaggatttc
     1801 aaagtatcct gaattactga cccactatgc cggttggtat tgatgttgac agcgtgacgg
     1861 gcactcaggc ccgccacagt atggtatctt aaataccgcg tcagtcgcag tatgttattc
     1921 aataaactgc tgcacatccg cttcgtaagc gtttttaata gcttgctggc tggagcggtc
     1981 gtaacccaga tacgctaata acaaatttcc ttcggaacgt ttcattttat cgaaatgctc
     2041 ttcgagcagt tccagtaatt tacaggcgtc atcattggcg acaaaccccg ttttataatc
     2101 attcacgtaa aggtagcctt tgtgatgcca caagcttgtt acgttgctcc ataaaacacg
     2161 gaacttgtca ggctccccgc ctaagcaggt aatatcatcg cgccctaaca taatcccatg
     2221 cttccctgag ccccacagcg tggtatcaaa caagacctta cccacagaaa aataaagcga
     2281 attgtcccgg cacgcctgat caaagttttt ccagcgcttt ttactggcat cttttctaaa
     2341 cgattgccat gaggtactgg cattttttcc gtcgcacagg tgatccggaa cgttatccag
     2401 gaagtcgacc atagtaagta cgtgcttctg gcaatagcta agcgtgattt ttttcaggcc
     2461 gttaacatag tcatctcttc caatgattgt gccgttgccg ctgtgctccg agagttcttc
     2521 accctgctgt agggcttcca gcatagctgc cgcctgctct ttttgtgagg tttcacgctg
     2581 gctgaggctg gcatcttcat agcgtttcag gcagtgtatg agcggcttca ggtgctggct
     2641 gaaaacctcc caatgatttt tgcttttatc gactgcgagt tcgttgtcga tatattgttt
     2701 tgtcaggtag ttaataaggc tgctgtcgtc tttgaaatcg gtaaattctt catcactgcg
     2761 acggtccttt tttttctctg tctccgaggc attggctaca gaacctgaga tcgcgcttaa
     2821 ggaatccatt agtgttttaa gaaaactcat ttatcctcac tacccagatt atttttgatt
     2881 gtatttaaaa tttgttcagc ttcagcaata tcctgctgct gatattcgat attggcttct
     2941 gcatacagat gcctaataaa ggcatctgtc tcatttttca gtgaatcaac cgacgctctc
     3001 agcgcgttgc gtgaatcctg ccagtagcgc gtgatatccg cttccacatt gcttaacgta
     3061 ttactggatt ccagcccctt aacaactgat ttggcgagtg cctttttcca gtttgcgaac
     3121 agtcgataga ccagtagccc tacaaccgcg gcaatagcta cgccccatgc caccgggcca
     3181 ccagtggcgg caacgagagt tggtaatgtc gtgaccgaac tggtaatccc tagggaagtc
     3241 agcacgccag ccgctttgcc aactaaaata taggcaccta aattgctcga tatagttgcc
     3301 acgtaacccg ccatagcgcc gaagctggtt aatccggcca tacctccgac gaaggcggag
     3361 tatgcatcaa agtcggaggt ggcggaaagg gaaatatcag tctccggcat tcccgcacgg
     3421 gctgcgcctt gggcggaagg cacgattttc tgccaggcct ggaccacgct ttccacctca
     3481 aggctgaacc tacgggtgct ctcttccatt acgcgacgaa atttgctttc gaaatgatta
     3541 cccagcgctt tattaatagc ccgtttcgct tcatctttat cttcaaagcg attgtcgatg
     3601 aaacggtaca tactctcatc attgcaaagc gagtagaaaa tatcgcggat gcgctcaata
     3661 tcttcctcgg cgtagcgcgg acagaagtta ctgatttcag taaagcgagc aattaacgcg
     3721 ccggaaccct cgcgaaatcg cgcatcctgt tgctcaatat cctcctttct ttcctgaaca
     3781 gaacggctac tctgctgtaa ttcctgcagc gctaatgcaa tcctgttttg gatgcgttta
     3841 tggatggtct ctatccggtt gttaatcagc gcagttcgat tggcaaggag ataattgatc
     3901 acctctttca ggcggacatc aaaggcttcg caatattgtc tattttcccg gaagaatggc
     3961 agcattctgc tggcccagac agcggcgtcg ggccgtagta atttctgtgg atggttggga
     4021 ttatttaact gatgctccca ttcatcatac agggtatgcg aaaaggtttt cgtcaggtga
     4081 ttcgcggcgg tacgaaacgt tttatggttc gcttcggcgg taatttgatc gtaactgtgg
     4141 gtttttaaga tgatcaaatt cgccagcggc ttgtctcctt ccagaggcac cttatgcatt
     4201 aaaatatttt gtaagaactc cggatcttcg tcacgcataa atttggtggt agcagatgcg
     4261 aagataaggc catctaccag cgaaattgcg ctcatggctt tggctttatc gttaaggatt
     4321 tcttcgctgg agcatccatc aatttcatcg tacatttcgg gttcaattaa ttgccccggc
     4381 gtatcaagta accagatagt ttccagaatc gcgaaacttt caaacaccac aaccatataa
     4441 gcttgtggat taaggacatt atcgttttca tccttttcgc cataggtttt gatgagctct
     4501 tttcctcctg aatcgatcag gaagtctgcc agcatttgac gatcaaaaat catatgcggc
     4561 ttgaaaccat ttttgaaaat aaagactgat ttattcttat ccagataatc aggtttatcc
     4621 tgagtgtgca tcagtagcgt gacaaggcag gtcgcgggtt gccatttcgc cgggagaatt
     4681 tcgctgccca aaatatagtt taacagcgta gactttcctg catctgatgg ccccatagcg
     4741 ctcaggacgg gcttggcgtg aatatcttta agattgtctt taagctcggc gatatccttg
     4801 gtgatgctgc tgtttttaat aaagggatta gaagtattgg acagcgtggc tatcgatctt
     4861 tccaggtact ctatcagttc ctttttattt ctcatcatat tctctttttg ctgtgtgtag
     4921 ttttgattta aagaggacgt gtttaactgc gtctttaaac aatcagtgat gaattcctga
     4981 atcttttcag gcgcaacgat gcgaaggtag gccagcacca gttctaaatt ttgtgacagc
     5041 ggtgcctgtt caatacgcga gacctgcttt tgggacagaa cattttcacc tgcccgctcg
     5101 gcgacttcct gttggcttaa acccgcctta tctcgccaca ttttcagttt atcgttcatt
     5161 acaatgccca ccatcttcag accggtatgt ctaaaatagc aatgaatgca tgatgaatcc
     5221 agacgtattt gtctaaaaaa aagaaccatc cgttagatca taatcgatat ccctagtaac
     5281 tacctgatat taaagatttg ttaatataaa atgccgaatg gacgaaactt attagtgata
     5341 agggttatcc gtagtattga aaaattcgtg atattaatgt gatctgttta ccataaatta
     5401 acatgattca gtgccagcaa tgtccgcttc tggcacagga ctgctgttgc agccctggca
     5461 accttagact tcaatcgatt cagagatctc taacgcatca tcgacttcaa tgcccagata
     5521 acggactgtg ctttccagtt tcttatggcc taacagaagt tggatcaccc tgagattctt
     5581 agttttcttg taaatcaggt aaggttttgt tcttctcaag gaatgtgtac tgtaaagcga
     5641 atcttcgaga ccaagttttg ctacccaccc atgaaaaatt cggtttgaag tggtcaacaa
     5701 aaactggcca ccgagttaga gttttttcca gtatcgattt tccgattcgt ttgggggtaa
     5761 cccaccgtta tattcgtgcg gtcttagtgc gctgtaatat ccaacgatat aatccgttat
     5821 ggcgtgagct gcctcgctga agcttacgta acccaccacc ggtatccatt cgttcttcag
     5881 acttctgaag aagcgctcca ttgggctgtt atcccagcag tttccgcgcc ggctcatact
     5941 ctgtctgatc tggtatcgcc acaataacta ccggaactgc ctgctcgtat aatgactgcc
     6001 ctgatcgctg tggaacatca ccccgccggg cttaccacgg gtttcccatg ccatttccag
     6061 cgctttcatg gtgagcctgc tgtccggcga gaacgacatg gcccagccca ctggttttct
     6121 tgcgaacagg tcgagaacaa cggcgaggta cgcccagcgc ttacccgtcc
//
LOCUS       DAPGRN010000198         6003 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPGRN010000198 DAPGRN010000000
VERSION     DAPGRN010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.200"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..203
                     /locus_tag="RHW33_004510"
     CDS             <1..203
                     /locus_tag="RHW33_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647940.1"
                     /translation="TIGGKLKVTQLSTTGYLGQFDFCAIARMGNAEDAHYCQVVESPA
                     GSRKWYKYEHKTGCIASCVTLN"
     gene            complement(200..448)
                     /locus_tag="RHW33_004511"
     CDS             complement(200..448)
                     /locus_tag="RHW33_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647941.1"
                     /translation="MFIYASGGNGGSAGGACANTSRLQGYVGGTLISVNASNNPAYGK
                     TAFISFAVPAGTSYQITSYPTENTSCGAGVFSVFGYQT"
     gene            complement(1617..1835)
                     /locus_tag="RHW33_004512"
     CDS             complement(1617..1835)
                     /locus_tag="RHW33_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HDV1647942.1"
                     /translation="MPTGFSASQCSWSVSNAENPQGWKPNYFAGSVATYDANRIVKCG
                     FYDEYNFHKGTFRADLTGKCSYVVACQN"
     gene            complement(1884..1970)
                     /locus_tag="RHW33_004513"
                     /pseudo
     CDS             complement(1884..1970)
                     /locus_tag="RHW33_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon protein D'"
     gene            1969..3123
                     /locus_tag="RHW33_004514"
     CDS             1969..3123
                     /locus_tag="RHW33_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV1647943.1"
                     /translation="MPSPRIRKMSLSRALDKYLKTVSVHKKGHQQEFYRSNVIKRYPI
                     ALRNMDEITTVDIATYRDVRLAEINPRTGKPITGNTVRLELALLSSLFNIARVEWGTC
                     RTNPVELVRKPKVSSGRDRRLTSSEERRLSRYFREKNLMLYVIFHLALETAMRQGEIL
                     ALRWEHIDLRHGVAHLPETKNGHSRDVPLSRRARNFLQMMPVNLHGNVFDYTASGFKN
                     AWRIATQRLRIEDLHFHDLRHEAISRFFELGSLNVMEIAAISGHRSMNMLKRYTHLRA
                     WQLVSKLDARRRQTQKVAAWFVPYPAHITTIDEENGQKAHRIEIGDFDNLHVTATTKE
                     EAVHRASEVLLRTLAIAAQKGERVPSPGALPVNDPDYIMICPLNPGSTPL"
     gene            3274..4098
                     /locus_tag="RHW33_004515"
     CDS             3274..4098
                     /locus_tag="RHW33_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDV1647944.1"
                     /translation="MRLNTTGIAARMMLSLDKKAIGEKLINGRQINPTPLQVRYPQGV
                     REVLGIMSEQLAISTADLTRILLEDALHNMFMPADNTTGNIISRIEYIMLSHDINAPL
                     LATLLSPWNIRSTVIQDPARLADYLSADALEHLAECFNLNPDWLNGHENYPIALSGEW
                     PDTADNFRMLISDSSNTEVIFWHSFPFAGNTKREYCGVILRQKKEINGSVIYPALSLS
                     PTLLNDEKRKWLTEYTTRQNTTMSLRRVTLRPGLAGNLITGQILPVSLFNTSLLPW"
     gene            4183..5385
                     /locus_tag="RHW33_004516"
     CDS             4183..5385
                     /locus_tag="RHW33_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDV1647945.1"
                     /translation="MMKKNHITRTIIASAVLFSFNAAAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKHNSNDDFSLLLPSVGAQVADPDDVSNKADDVKDDWDLFDSAVD
                     NQHGVQQAAANLKHRLQEFRNINADAQVGVSAVAAMANDTLPFALMVKSYGTVSVNGK
                     VNDADLDYLDKVANGTITDVDKNALTSRAFGRAAVITDVGISFAKELETAGQKWSLGV
                     TPKYQRVDLFNYNVTVRDYDKDDFDGDKYHNTKNGFNADIGAYTDLNDNWTVGLVAQN
                     IIPRSIDTKVVNGFKETFKVRPQATAGVSWHNDLFTTALDIDLTPASGFTSDSKRQFA
                     SVGAEFNAWKWAQLRAGYRQNMASNSGSAFTAGVGISPFDVVHIDVSGLVGTDHDYGA
                     MAQLQFTF"
     gene            5445..5888
                     /locus_tag="RHW33_004517"
                     /pseudo
     CDS             5445..5888
                     /locus_tag="RHW33_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542524.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
BASE COUNT         1731 a         1419 c         1289 g         1564 t
ORIGIN      
        1 ggacaatagg tggaaaactc aaagttactc agctttccac cacaggttat ctggggcaat
       61 tcgacttctg tgccattgcc agaatgggca acgcagagga tgcccactac tgccaggtag
      121 ttgagagccc agcaggttca cgcaaatggt acaaatacga gcataagaca ggttgtattg
      181 cgtcgtgtgt aacgctcaat taagtttgat atccaaatac tgaaaatacc ccggcaccac
      241 atgatgtatt ttctgttgga taggatgtta tctgatagga agtacctgca ggtacagcaa
      301 agctgataaa ggctgttttt ccatatgcag ggttattgct ggcattcacg ctaattagcg
      361 tcccaccaac atatccctgc agtcgggatg tatttgcaca tgcacctccg gcagatcctc
      421 cattacctcc agatgcataa ataaacaatg tactgccccc tgaattccgc cctgagaatg
      481 aacctctatg tgaacctaag tttgtgtaag aaccattgag cgaaccagaa gtcttccacg
      541 taccggattg gcacgtaggc cagtgccaat ccggtacctg gaaatcatca tcagcgtcga
      601 tatggacgaa tataaaaaca tttacgttat atcccaaaaa cacacaggtc ttagggaggt
      661 ttaaactgtg tattaacacc tatcgtattg acgggcgtga aatggcagaa acagaagtcg
      721 ttcctattga catgcctgac tctaacggcg agatgacttg gcaagccaaa aactacacac
      781 agtattcgtc ctactttatg aagattactt gcttgaaata gcttattggc aaatggcata
      841 tacagttatt gaaccattct gatcttcggt tgtatcgcac gcagccgacc atgagttatt
      901 ggctgatggg aatgacctgg taagccatat gtaaccagtt cgtgaggtgc atattccgcc
      961 gccacctgta acacgttttc cagttggaca agtagctgtt gctccaggaa agcgccaacc
     1021 tgtggcctgt gctgtagcaa tttgaagctc accagatcct acctttctcc aggtaccgga
     1081 ttggcacgta ggccagtgcc aatccggtac ctggggcgct cctaaaattc aattcacaac
     1141 gcagacctac aacatagcta aaaacactcg taatttaagg ctcggggtcc atgcatattg
     1201 ctcatggact tacctgaatg gctcaccctt tggtggtttt caacaggtat attccgacca
     1261 aaacaacgtt tggtatgtga gtaattatgc ttggggaaat tatgagtctg gtgggaccat
     1321 atcagtcaca tgcctcaatc ttcctggtgc tggagcttaa ttaaaggggc aacagtaggc
     1381 atcgacatgt tcctcatcaa caccatcacc aataccgtac atgcgtgttc cagtcataat
     1441 gtatccagtc ggacattgaa tcggcttgta atagatcgaa ccaccactct taccgccgat
     1501 aaagtgattc attgctacgg atgatttgta ccaattacat gctgagtaat taattttatt
     1561 accccctgac caccgaccgg attggcacgt aggccagcag cgccagcaat ttaatctcag
     1621 ttctgacacg cgacgacata actacatttt ccggttaaat cagctctaaa tgtcccttta
     1681 tggaaattgt attcatcata aaaaccacat ttaacaattc gatttgcatc ataagttgca
     1741 actgatcctg caaagtaatt cggcttccaa ccttgagggt tttctgcatt gctcacactc
     1801 catgagcact gactggcaga aaatcctgtt ggcagcggta tttgctgacc atttgctata
     1861 cgaccagtta tcaccgtact accagaattg cttttacgcc aagtaccgga ttggcacgct
     1921 tgcagagaat gattgttggc attatttttt gcctcttttg aggttagcat gccgtctcca
     1981 cgcatccgta aaatgtccct gtcacgcgca ctggataagt acctgaaaac agtttctgtt
     2041 cacaagaaag ggcatcaaca ggagttttac cggagcaatg ttatcaagcg atatcccatt
     2101 gcccttcgga atatggacga aataacaacc gttgatattg ctacatacag agacgttcgt
     2161 ttagcagaaa tcaacccccg aacgggtaaa cccattacag gtaatactgt acgtcttgaa
     2221 ctcgcccttc tgtcatctct gttcaatatt gctcgtgttg aatggggaac ctgtcgtact
     2281 aacccggttg aactggttcg caagccgaaa gtatcctcgg gacgagatcg ccggctaacg
     2341 tcttcagaag aacgtcgcct ttctcgctat ttccgcgaaa aaaatctgat gttgtatgtc
     2401 attttccatc ttgcccttga gacagccatg cggcagggcg aaatactggc cttacgttgg
     2461 gagcacattg atttgcgcca cggtgtggct catttacctg aaaccaaaaa cggtcactca
     2521 cgggatgttc ctctgtccag acgtgcccgt aactttcttc agatgatgcc cgttaatctc
     2581 cacggcaatg tttttgatta caccgcatcc ggctttaaaa atgcctggag aatagccaca
     2641 caacgacttc gcatcgagga cctgcatttt cacgatctac ggcatgaagc aataagccgc
     2701 ttcttcgaac tgggtagcct gaatgtaatg gagattgctg caatatcagg acatcgttcc
     2761 atgaatatgc tgaaacggta tactcatctt cgtgcatggc aactggtcag taagcttgat
     2821 gcccgccggc ggcagacaca aaaagtggca gcatggtttg tgccgtatcc tgcccatatc
     2881 acgaccatcg atgaagaaaa tgggcagaaa gcgcatcgta ttgagatcgg tgattttgat
     2941 aaccttcacg tcactgccac aacgaaagag gaagcagttc accgcgccag tgaggttttg
     3001 ttgcgtacac tggccattgc agcacagaaa ggcgaacgtg tcccatctcc cggagcgtta
     3061 cctgttaacg accctgacta cattatgatt tgccctctga acccgggcag caccccgctg
     3121 taaacatcat tcaaaacaca tacagaaagg cgagttgcaa caactcgcca aacacattcg
     3181 atctgcatga tcgaaattat tcgtttcacc agaaaaaaca ccgaagatac aataaccaca
     3241 tgagctgaaa agactttttc agtcggagga acaatgagac tgaatacgac cggtattgcg
     3301 gcccgaatga tgctctcgct ggacaaaaaa gcaatcggcg aaaaactcat caacggtcgt
     3361 cagataaacc cgacacctct gcaggtccgt tacccccaag gggtaagaga agttctgggg
     3421 ataatgagcg aacaattagc catatccacc gctgatttaa cccgaatcct gcttgaggat
     3481 gctcttcata atatgtttat gcctgcggat aacaccacag gaaacataat aagcagaatt
     3541 gaatacatca tgctgtcgca tgatataaat gcgccattac tggcaacatt gctgtcgcca
     3601 tggaatatcc gctccacggt tatacaggac ccggccagac ttgcagacta tctttcagct
     3661 gacgctctcg aacatcttgc tgaatgtttt aatctcaatc cggactggct gaatggccat
     3721 gaaaactatc cgatagcgtt gtccggtgaa tggccagata ctgctgataa tttcagaatg
     3781 cttattagtg atagcagcaa cactgaagtt attttctggc atagtttccc cttcgcagga
     3841 aatacgaaaa gagaatattg cggggtaata ttgagacaga aaaaagaaat aaatggctct
     3901 gtaatatatc cggcactatc attatcacca acacttctga acgatgaaaa aagaaaatgg
     3961 ttaactgaat atacaaccag gcagaataca acaatgtctt tgcgacgcgt cacgttgaga
     4021 cctggccttg ccgggaacct tattacaggg caaatactac ctgttagctt atttaatacg
     4081 tctttattac cctggtaaat accagatagt accaaagtaa aaattacggc acatccgtgt
     4141 catagcgttg ttttgtctaa attctgataa acaggaaatt aaatgatgaa gaaaaatcat
     4201 attacccgca ccatcattgc atctgctgta ttattttcct ttaacgctgc agcagcgacg
     4261 tcatattttg aagcgcgtaa cgatgcaatg ggtggtaccg gggtagcatc ttcccattat
     4321 ggtgtggcac ctctggcaaa cccggcttta ctgacaaagc acaacagcaa tgatgatttc
     4381 agccttcttc tgccgtctgt tggggcacaa gttgctgatc ctgatgatgt atcaaataaa
     4441 gctgatgatg ttaaagatga ctgggatttg tttgacagtg ctgtagataa ccagcatggc
     4501 gtacaacagg cagcggcaaa tcttaaacat cgtctgcagg aattccgcaa tatcaatgca
     4561 gatgctcagg taggtgtatc agctgtagca gccatggcaa atgacactct gccgtttgcc
     4621 cttatggtca agtcttacgg aactgtcagc gtaaacggaa aagtaaacga tgcagacctg
     4681 gattatctgg acaaagttgc caatggaaca attaccgacg tcgataagaa tgccctgacc
     4741 tcacgcgcat tcgggcgtgc agctgtaatt accgacgtcg gaatttcatt tgccaaagaa
     4801 ctggaaacgg ccggacaaaa atggtctctt ggcgtcacac caaaatatca gcgtgtggac
     4861 ctgtttaact acaacgtaac ggttcgtgat tacgacaaag acgattttga cggtgacaag
     4921 taccacaaca cgaaaaacgg attcaacgcc gatattggtg cttatacaga cctgaatgac
     4981 aactggactg tcggtcttgt tgcccagaac atcattccac gcagtattga tactaaagtc
     5041 gtcaatggct ttaaagaaac cttcaaagtt cgtccgcagg caaccgccgg tgtatcctgg
     5101 cataatgacc tgtttaccac tgctcttgac attgatctga ctcctgccag tggcttcact
     5161 tctgacagca aacgtcagtt tgcaagtgtt ggtgctgaat ttaacgcatg gaaatgggcg
     5221 caattgcgtg ccggttatcg tcagaacatg gcatcaaact ccggaagcgc cttcaccgct
     5281 ggcgttggta tctctccatt tgacgtcgta catatcgatg tttccggcct ggtaggaact
     5341 gaccacgatt acggtgcaat ggcacaactt cagttcactt tctgattact gtccccgccc
     5401 ccggcactaa acccgggggt atttttatat tcagtttaaa taacatgatg aaaccacgtt
     5461 cttcttattc aaaaacagca ttcattcttt tattttctgt tttcctggta gcggcagtga
     5521 caaaagcaaa aagctcgctt cccgatatca ccctggaaca ggcaaaagaa attaatgctg
     5581 ataacaccgt tattttcctt ttccgccatg gagaacgttg cgaccgttca gatatgccat
     5641 gctattcgga taaaagcgga atcactatta ccggtacaga gaaagcacag caggaaggta
     5701 ttaagttcgc aacaatcttc agtgaatatg atatttactc cagcaatgcg gtaagaacca
     5761 tacaaactgc aaaattcttc tccggtaaag aacccgtcgt tatggacagt ttatcagact
     5821 gcaataatga tctttataag acgcttgaat caatcgcaag agaatctcat aaaaggaaca
     5881 tagtaatacc tagattctac gtcagtactt caaaaagcat aatcaaagcc ttgataaata
     5941 tgcattcctt cgaaattcag ctttcaccca ttgggtgaaa gaaaagtgct caaaaatatg
     6001 tta
//
LOCUS       DAPGRN010000199         5963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPGRN010000199 DAPGRN010000000
VERSION     DAPGRN010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.201"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            211..738
                     /locus_tag="RHW33_004518"
     CDS             211..738
                     /locus_tag="RHW33_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1647946.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            796..1029
                     /locus_tag="RHW33_004519"
     CDS             796..1029
                     /locus_tag="RHW33_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV1647947.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1090..3113
                     /locus_tag="RHW33_004520"
                     /pseudo
     CDS             1090..3113
                     /locus_tag="RHW33_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3182..3616
                     /gene="psiB"
                     /locus_tag="RHW33_004521"
     CDS             3182..3616
                     /gene="psiB"
                     /locus_tag="RHW33_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV1647948.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3613..4332
                     /locus_tag="RHW33_004522"
     CDS             3613..4332
                     /locus_tag="RHW33_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV1647949.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARV
                     VGHAVREMERWMVPNKLREAA"
     gene            complement(4344..4532)
                     /locus_tag="RHW33_004523"
     CDS             complement(4344..4532)
                     /locus_tag="RHW33_004523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HDV1647950.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4589..5958
                     /locus_tag="RHW33_004524"
     CDS             join(4589..5069,5069..5958)
                     /locus_tag="RHW33_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HDV1647951.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RISEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQ
                     TYMPRV"
BASE COUNT         1478 a         1472 c         1774 g         1239 t
ORIGIN      
        1 ttacccaaaa cccggtaaaa aattttgcgt tctgcccagg acaggtgcgt caggccgtgg
       61 cagtgatgcc ccttgcggcg aaggttctca aacaggtgat ggtgaatacc ggagggaaca
      121 aaaggcaggt ccccgcaaaa cgtcaaaacc gcggctcccg aaactcaaca gatgatgggg
      181 gctgaaaaca tcaacagaag gagacacatc atggcagttc gtggcattaa caaggtcatt
      241 cttgtcggtc gtctgggaaa agatccggaa gtccgttaca tccccaacgg gggcgcagtg
      301 gcaaacctgc aggtggccac gtcagaaacc tggcgcgaca aacagacggg ggaaatgaag
      361 gagcagacgg aatggcaccg cgtggtgctg ttcggcaagc tcgcggaagt ggcaggtgaa
      421 tatctgcgta agggggcgca ggtctacatt gaaggccagc ttcgcacccg tagctgggaa
      481 gataacggta ttacccgtta cgtcaccgaa atccttgtta aaaccacggg caccatgcag
      541 atgctgggga gtgcaccaca gcagaacgct caggtgcaac cgcagcctca gcagaatggt
      601 cagtcacaga gtgctgacgc gacgaaaaaa gggagcgcga aaacgaaagg ccgtggacgt
      661 aaggctgcgc agccggagcc tcagccgcaa ccaccggagg gggaggatta cgggttttca
      721 gacgatatcc cgttctgact ggcctgactg tgataaccgc cccgtcctgc gcggggcatc
      781 accggagata tgaggatgag cgaatatttc agaatacttc agggactgcc tgacggtcct
      841 tttacccgca aacaggccga agccgttgcc gcacagtacc ggaacgtctt tatcgaggat
      901 gaccacggcg aacagtttcg cctggttgtc cgtaataacg gaacaatggt ctggcgtacc
      961 tggaattttg aggacggtgc cgggtactgg atgaaccatg tcatccgtga tttcgggatc
     1021 cttaagtaag agaggtgccg gacggggatc acgtccggca gacattaact acacaataag
     1081 gggatagtca tgtcagtaac aaagtctgaa ccaaaatcta cccgtaaagc aagccgtaaa
     1141 cctgcaaaaa cgcaggaaac tgtcctgtct gccctgctgg cgcagacgga ggaagtgagc
     1201 gtgccgctgg cctcgctgat taagtcacca ctgaatgtgc gcacggtgcc gtattctgcg
     1261 gagtccgtca gcgaactggc tgattccatt aagggggtcg gcctgctgca gaatctggtt
     1321 gttcatgccc tgccaggtga ccgccacggt gtcgccgcag gtggtcgccg actggcagca
     1381 ctcaacatgc tggcagagcg tggcatcatt ccggctgact ggcctgtccg cgtgaaaatt
     1441 atttcgcagg aactggcgac ggccgcatcg atgaccgaga acggtcatcg tcgggatatg
     1501 cacccagccg aacagattgc cggattctgt gcaatggcgc aggaaggcaa aacacctgca
     1561 caaatcggtg atttgctggg ctattcaccc cgccacgttc agcgaatgct gaaactggct
     1621 gaccttgcgc ctgtcatcct cgatgcgctg gcagaagacc tcatcaccac cgagcactgt
     1681 caggcgctgg cgctggagaa cgacaccgcg cgtcaggtgc aggtgtttga agccgcctgt
     1741 cagtcgggat ggggcggtaa accggaagta cagaccattc gtcgtctggt gaccgaaagt
     1801 gaagtggcgg tggcagggaa cagtaaattc cgcttcgtgg gggctgatgc cttctcgcca
     1861 gatgaactgc gcactgacct gttcagcgac gacgagggtg gctatgtgga ctgcgtggcg
     1921 ctcgatgccg ccctgctgga aaaactccag gctgttgctg aacaccttcg ggaagccgaa
     1981 ggctggggat ggtgcgccgg acgcatggag cctgtcggtg agtgccgtga ggatgccgga
     2041 acataccgct gtctgccgga gccggaagcg gtgctgacgg aggcggaaga agaacgtctg
     2101 aacgaactga tgacgcgtta cgacgcgctg gaaaaccagt gtgaggaatc tgacctgctg
     2161 gaagcagaaa tgaagctgat gcgctgcatg gcgaaggtca gggcgtggac gccggagatg
     2221 cgtgccggaa gcggtgtggt ggtgtcctgg cgttatggca acgtgtgtgt ccagcgtggt
     2281 gtgcagttgc gcagcgagga tgatgcggct gacgacgctg accgcacgga acaggtgcag
     2341 gagaaagcgc ccgtggagga aatcagtctg ccactgctga cgaaaatgtc ttccgaacgc
     2401 acgctggcag tccaggcagc actgatgcag caaccggaca aatctctggc actgctggca
     2461 tggacgctct gccctgatga atcccctaat gatttttacc aaaatcatta ggggattcat
     2521 cagcgctctg cctgaatgtg tttggcagcg gagcgtacag taaaccagca caaatcagcc
     2581 tggaatgtaa acattattcg ctgaccagcg atgcgccatc ggggaaggac ggtgccgcat
     2641 tcctggcgct gatggcagaa aaagcccgtc ttgcagccct gttaccggag ggatggtcac
     2701 gggatatgac gacattcctg tcactcagcc aggaggtact gttatccctg ctcagtttct
     2761 gcaccgcgtg cagcatccac ggcgttcaga cccgcgagca tggtcacacg tcacgcagtc
     2821 cgctggactc gctggagagc gctatcggat tccacatgcg cgactggtgg cagccgacga
     2881 aagcaaactt cttcggacac ctgcaaaagc cgcagattat cgatgccctg aatgatgccg
     2941 gactgtccgg tgctgcgcgg gacgcggaga aaatgaagaa aggcgatgcc gctgaacatg
     3001 cagagttcca catgaaagac aaccgctggg tgcctggctg gatgtgtaca ccacgtccac
     3061 aggcagagac ggaaaccacg gagtaccgtg acgaccaggc agaagccgcc tgaccgccag
     3121 tcataaacca tttatcacgc cgccctgccg gagacggggc ggcagcaagg gagataccgt
     3181 gatgaaaact gaactgaccc tgaatgtatt acagaccatg agcgcacagg aatatgaaga
     3241 tatccgggct gccggaagtg atgaacgtcg tgaactcaca catgccgtga tgcgggagct
     3301 ggatgcgccg gataactgga cgatgaacgg cgagtatggc agcgagttcg gcggcttttt
     3361 ccccgtccag gtccgtttca cgcctgccca cgaacgtttt cacctggcgt tatgttcacc
     3421 gggcgatgtc tctcaggtct gggtgctggt tctggtgaat gccggtggtg agccgtttgc
     3481 ggtggttcag gtgcagcggc ggtttgcacc ggaagccgtc agccattcac tggcactggc
     3541 agcgtcactg gacgcgcagg ggtacagcgt cagtgacatc atccatatcc tgatggcgga
     3601 aggaggtcag gcatgagcgc acgttcacag gcactggttc ctctcagcac agagcaacag
     3661 gccgcatggc gggcggtggc ggagaccgaa aaacgccgcc atcagggtaa cacgctggct
     3721 gaatatccgt atgcgggggc gtttttccgc tgtctgaacg gaagccgcag gatatcgctg
     3781 tctgacctgc gatttttcat gccgtccctg accgcagaag aactgcacgg caaccgtctt
     3841 cagtggctgt atgccatcga tgtgctgatt gaaacacagg gcgaggtctg cctccttccc
     3901 cttcccggtg atgcagcaga gcgcttgttc ccgtcggtgc ggtttcgtgt cagggaacgc
     3961 agtcgccata aatccgcgct ggtcatgcag aagtacagcc gccagcaggc gagggaggca
     4021 gagcagaaag cccgtgcgta ccaggcgctg gtcgctcagg ctgaaatcga actggcattc
     4081 cattcgccgg aaacagtcgg aagctggcat gcacgctgga gtgaccgggt ggcagagcat
     4141 gacctggaaa ccctgttctg gcagtggggc gagcgttttc cgtcactggc cggaatggaa
     4201 cgctggcagt ggcaggatat gccattctgg caggtcatcg cagaggctgg tatggcggca
     4261 agggtggtcg gtcatgctgt tcgtgagatg gagcggtgga tggtgccgaa caaactccgg
     4321 gaggcagcgt gatgcggcaa caatcacacg gatttcccgt gaacggtctg aatgagcgga
     4381 ttattttcag ggaaagtgag tgtggtcagc gtgcaggtat atgggctatg atgtgcccgg
     4441 cgcttgaggc tttctgcctc atgacgtgaa ggtggtttgt tgccgtgttg tgtggcagaa
     4501 agaagatagc cccgtagtaa gttaattttc attaaccacc atgtactgca cccattttgt
     4561 tggacgatga aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg
     4621 ccttgaagtc gtgaatcact acttcacaac tgatgatggt tacaggatca tctcggcacg
     4681 ttttggtgtc ccccgaaccc aggtcaggac atgggttgcc ctctatgaaa aacatggaga
     4741 aaaaggttta attcccaaac ctaaaggcgt tagtgctgat ccagagttgc gtattaaggt
     4801 cgtgaaagct gtgatcgagc agcacatgtc ccttaatcag gctgctgctc actttatgct
     4861 tgctggtagt ggttctgtag ccaggtggct gaaggtctat gaagagcgcg gagaagctgg
     4921 tttacgcgcg ctcaagattg gcaccaaaag aaacattgca atatcagttg atccagaaaa
     4981 agcggcatca gcattggagc tgtcaaaaga ccgacgcatt gaggatcttg aaaggcaagt
     5041 tcgatttctt gaaacgcggc ttatgtatct aaaaaagctg aaagccttag ctcatcccac
     5101 gaaaaagtga aagtactcaa cgagctaagg cagttttatc ctcttgatga gcttctcagg
     5161 gctgcggaga taccgcgcag tacgttttat tatcatctaa aggctctcag caagcctgac
     5221 aagtatgcgg acgttaaaaa gcgtattagt gagatttatc acgagaatag aggccgatac
     5281 ggataccgta gggtaacgct gtctcttcat cgagaaggga aacagattaa ccataaagct
     5341 gttcagcgcc tgatgggaac cctctcactt aaagcagcga ttaaggtcaa gcgataccgc
     5401 tcttacagag gagaggtagg gcaaaccgcc cctaatgttc tccaaagaga tttcaaggct
     5461 acgcggccaa acgagaagtg ggttaccgat gttactgaat ttgcagtcaa tgggcgcaag
     5521 ctgtatttgt ctccagtaat agatctcttc aacaacgaag ttatttctta cagcctttcg
     5581 gaaagaccag tgatgaacat ggttgagaat atgctcgatc aggcattcaa aaagcttaat
     5641 cctcacgagc atcctgttct gcactctgac cagggatggc agtatcgtat gagaagatat
     5701 caaaatatcc ttaaagaaca tggtattaaa caaagcatgt ccagaaaagg caattgtctg
     5761 gataatgctg tggtggagtg tttctttgga accttaaagt cggagtgttt ttatcttgat
     5821 gagttcagta atataagcga actgaaggat gctgttacgg aatatattga atactacaac
     5881 agcagaagaa ttagcctgaa attaaaaggt ctgactccaa ttgaatatcg gaatcagacc
     5941 tatatgcctc gtgtttaact gtc
//
LOCUS       DAPGRN010000200         5942 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPGRN010000200 DAPGRN010000000
VERSION     DAPGRN010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.202"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..312
                     /locus_tag="RHW33_004525"
     CDS             <1..312
                     /locus_tag="RHW33_004525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647952.1"
                     /translation="SGKVEFNSEQLSKLMAEITSFESNLDGTPKDINFISQNEHLRQT
                     VSIRQDGLCTKITLIVEETHFDIDHGDILHGKKWSETRVLPEELNMAIVVENLRNLPH
                     S"
     gene            complement(514..1050)
                     /gene="ssb1"
                     /locus_tag="RHW33_004526"
     CDS             complement(514..1050)
                     /gene="ssb1"
                     /locus_tag="RHW33_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV1647953.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQLQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            1305..4127
                     /gene="uvrA"
                     /locus_tag="RHW33_004527"
     CDS             1305..4127
                     /gene="uvrA"
                     /locus_tag="RHW33_004527"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV1647954.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(4162..4518)
                     /locus_tag="RHW33_004528"
     CDS             complement(4162..4518)
                     /locus_tag="RHW33_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV1647955.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4522..4938)
                     /locus_tag="RHW33_004529"
     CDS             complement(4522..4938)
                     /locus_tag="RHW33_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV1647956.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(5049..5762)
                     /gene="aphA"
                     /locus_tag="RHW33_004530"
     CDS             complement(5049..5762)
                     /gene="aphA"
                     /locus_tag="RHW33_004530"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV1647957.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     SMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1464 a         1529 c         1538 g         1411 t
ORIGIN      
        1 agtgggaaag tcgagtttaa ttctgagcaa ttatctaaac tcatggcaga aataacctct
       61 ttcgagtcca atcttgatgg aacccccaaa gatataaatt ttatctctca aaatgaacat
      121 ctacgccaaa ctgtatccat cagacaagat gggctatgca cgaaaataac actcattgta
      181 gaagaaaccc attttgatat agaccatggc gatatcttac atggaaaaaa atggagtgaa
      241 acaagagtgc tgcctgaaga attaaacatg gctattgttg ttgaaaacct tagaaactta
      301 ccacacagtt aaacaaagtt gagcaacatg aaatagagag attatagaag atataaaccg
      361 gtacaaatag caaaatcaga tattttgtaa aatccggtat aataaattaa tgagaggaac
      421 aatccttgac tatttcagga gccgaaattc attttcgact cctggacgca tatgtttcgc
      481 gttgagagat ctcaacacaa aagatgcaga ttcttagaac ggaatatcgt catcaaagtc
      541 catcggtggc tcgttagacg gcgctgccgg agcggactgc tgcgggcgag actgcgcgcc
      601 gccgctgaac tgattgccac cctgcagctg ctgaggctga ccccaaccgc cctgcggctg
      661 accaccaccg atattgccac ctgcaggagc gccaccaccc tgacgaccac ccagcatctg
      721 catagtgccg ccaacgttta ccacaacttc tgtggtgtag cgatcctgac cagattgatc
      781 ggtccattta cgggtacgca gctgaccttc gatataaacc tgagaacctt tacgcagata
      841 ttcgctggcc acttctgcca gtttgccgaa cagcacaacg cggtgccatt cagtctgctc
      901 tttcatctcg ccggtcgctt tatcacgcca ggattcggaa gtagccagcg taatgttggc
      961 aactgcgcca ccatttggca tgtagcgtac ttccgggtcc tgacccagat taccaacgag
     1021 aataaccttg tttacgcctc tgctggccat gttcgtgtct cctgaaaaaa aatcgttctg
     1081 aataagtgta aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc
     1141 ggttccggga aacaaacctg gccagacatt gttacacaac actccgggta atgcattcca
     1201 atactgtata ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag
     1261 caccatgcca ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag
     1321 ttcggggcgc ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc
     1381 tcattgtcgt gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat
     1441 atgccgaagg gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt
     1501 cactgatgga aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg
     1561 agcagaaatc gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg
     1621 actatttgcg tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc
     1681 cgctggcggc gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca
     1741 agcgtctgat gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc
     1801 tggagaacct ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc
     1861 tttccgatcc gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc
     1921 gcttcaaggt gcgtgacgat cttacccaac gtcttgccga gtcgtttgaa accgcgctgg
     1981 agctttctgg tggtaccgcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc
     2041 tgttttccgc caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc
     2101 gactgttttc gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc ctcggcgtac
     2161 agcaatattt cgatcctgac cgcgtgatcc aaaacccgga actgtcgctg gcaggtggtg
     2221 cgatccgtgg ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag
     2281 atcactataa gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag
     2341 tggtgttgta cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg
     2401 atacctccat tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata
     2461 aagagacgga gtccagcgcg gtacgcgaag aattagccaa gttcatcagc aatcgcccgt
     2521 gcgccagctg cgaagggacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata
     2581 cgccgctgcc cgccatctcc gacatgagca ttggtcatgc gatggaattc ttcaacaatc
     2641 tcaaactcgc aggtcagcgg gcgaagattg cggaaaaaat tcttaaagag atcggcgatc
     2701 gtttgaaatt cctcgttaac gtcggcctga attacctgac gctttcccgc tcggcagaaa
     2761 cgctttctgg cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg
     2821 ttggcgttat gtacgtgctg gacgagccgt ctatcggcct gcaccagcgc gataacgagc
     2881 gcctgttggg tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc
     2941 acgacgaaga cgcgattcgc gccgctgacc atgtgatcga cattggcccg ggcgcaggtg
     3001 tacacggcgg tgaagtggtc gcggaaggtc cactggaagc gattatggcg gtgccggagt
     3061 cgttaaccgg acagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc
     3121 cggcgaatcc ggaaaaagtg ctgaagctga ccggtgcacg cggcaacaac ctgaaggacg
     3181 tgacgctgac gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta
     3241 aatcgacact gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga
     3301 ccatcgccga accggcaccc tatcgcgata ttcagggact ggagcatttc gataaagtga
     3361 tcgatatcga ccaaagccca attggtcgta ctccgcgttc taacccggcg acctataccg
     3421 gcgtgtttac acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt
     3481 atacgccagg acgtttcagc tttaacgtcc gtggcggacg ctgcgaagcc tgtcagggcg
     3541 acggcgtgat caaagtggag atgcacttcc tgccggacat ttacgtaccg tgcgaccagt
     3601 gtaaaggtaa acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc
     3661 acgaagtgct ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc
     3721 tggcgcgtaa gctgcaaacg ttgatggacg ttggcctgac gtacattcgc ctggggcagt
     3781 ccgcaaccac actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa
     3841 aacgcggcac cgggcagacg ttgtatattc tcgacgagcc gaccaccggt ctgcacttcg
     3901 ccgatattca gcaactgctc gacgtactgc ataaactgcg cgatcagggc aacaccattg
     3961 tggtgattga gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac
     4021 cagaaggcgg cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg
     4081 agtgcgaagc ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc
     4141 tttccgagcg gccttttcct ttcagagttg caccagcaat ttacgttttt cttccggcag
     4201 taaattcacc gcctgctgat aagacgcatc caccagataa tagatttgcg aatctggcag
     4261 cgaaccgtcg agatacacgg tgctccagtg cgctttattc agatggcggc ttggacgcac
     4321 atcgctgtgc tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc
     4381 tgggcgattt tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt
     4441 cgctttccag tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag
     4501 caactccgaa atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc
     4561 gccgtggatg cgatgttccc ccagccaaat gccttgccag gtgccggtct gaatacgccc
     4621 tttatgcacc ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat
     4681 atcgtctgct ccctcgtagt catgctcata atttccgttg tcgggaacgg tgcggaggaa
     4741 aaaacgctcc atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc
     4801 ggaggtatgt tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag
     4861 ctgattcaga atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag
     4921 cgtcttttga taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa
     4981 gacagcgaaa ggataaaggt gtgattaaaa aaacagcatt cgggagagtg tcacgctctc
     5041 ccgctctgtc agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc
     5101 aagggtttgt aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg
     5161 cgtgcagcgg taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc
     5221 agccattgcg attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg
     5281 ctggtggcag gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc
     5341 gggctacgac cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc
     5401 tggcgagcga cctcttttgg aatgctgaat tcatcccagc cgttgttcat tttttcccag
     5461 aacacaggat ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag
     5521 aagcccgggc tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga
     5581 cgccctgcga ggctattttc aatttgtgcg accgaaaccc aatgaattgg ggcctgttca
     5641 gcaagcctgg caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca
     5701 gcggaactgt ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc
     5761 atatttttcc ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa
     5821 ccatctgacc ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg
     5881 aaaagcagga tgattatatt taacccagta aatacgttcc tgtcgttttt ttgtaaaaaa
     5941 at
//
LOCUS       DAPGRN010000201         5849 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPGRN010000201 DAPGRN010000000
VERSION     DAPGRN010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.203"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            71..1237
                     /gene="nhaA"
                     /locus_tag="RHW33_004531"
     CDS             71..1237
                     /gene="nhaA"
                     /locus_tag="RHW33_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV1647958.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1303..2202
                     /gene="nhaR"
                     /locus_tag="RHW33_004532"
     CDS             1303..2202
                     /gene="nhaR"
                     /locus_tag="RHW33_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV1647959.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2240..3199)
                     /locus_tag="RHW33_004533"
     CDS             complement(2240..3199)
                     /locus_tag="RHW33_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HDV1647960.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCGLTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNFNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(3212..5662)
                     /locus_tag="RHW33_004534"
     CDS             complement(3212..5662)
                     /locus_tag="RHW33_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV1647961.1"
                     /translation="MLRTTRWVAAIIFLYSFPGYAEETFDTHFMIGGMKGEKVSEYHF
                     DNKQPLPGNYELDFYVNNQWRGKQDITIPESPVKPCLTKVLLTKLGVKTGNLNTEDNC
                     ILLDEAVHGGQYQWDISEHRLNLTVPQAYINELERGYVPPESWDRGIDAFYTSYNLSQ
                     YRSYDSNNNSHTASYGRFNSGLNLFSWQLHSDASYSKPDDMKGKWQSNTLYLERGWSQ
                     ILSTVQIGENYTSSLIFDSLRFSGIRLFRDMQMLPDSMQSFTPLVQGVAQSNALITVS
                     QNGYTIYQKEVPPGPFTIADLQLSGSGSDLDVSIKEADGNVRSFLVPYSSVPNMLQPG
                     ISNFDFIAGRSQIYGVKNQEDFLEANYIYGLNNLLTLYGGTILSDNYNAITLGNGWNT
                     PLGAISFDATRSSSKLNNDTRHEGTSYQVAYNKYLLQTATHFSVAAWRYASQDYRTFS
                     DHLYENDKINHQSDYDDFYDIGRKNSLSANIMQPLSNNLGNVSLSALWRNYWGRSGNA
                     KDYQFSYSNNWQHISYTFSASQSYDENDKEEERFNLFISIPFYWGDDIAKTRHQINLS
                     NSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNNDTSLGTNLSWRTPIATID
                     GSYSHSKNAWQSGGSISSGLVVWPGGINITNQLSDTFAILDAPGLEGAHINGQKYNRT
                     NNKGQVVYDLMIPHRENHLVLDTANSESETELQGNRQIIAPYRGAVSYVQFTTDQRKP
                     WYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLF
                     CTITFQHVIDENKTYICQ"
     gene            complement(5675..>5849)
                     /locus_tag="RHW33_004535"
     CDS             complement(5675..>5849)
                     /locus_tag="RHW33_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV1647962.1"
                     /translation="ITVTTIKAQNVKINNESILLPPFSNNDITLKNNHASEYELTVVD
                     DYGNNIHSKIAAR"
BASE COUNT         1529 a         1208 c         1321 g         1791 t
ORIGIN      
        1 tgattttaac gatgattcgt gcggggtaaa atagtaaaaa cgatctattc acctgaaaga
       61 gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt gatgcctcgg gaggcattat
      121 tcttatcatt gccgctatcc tggcgatgat gatggccaac agcggcgcaa ccagtggatg
      181 gtatcacgac tttctggaga cgccggttca gctccgggtt ggttcactcg aaatcaacaa
      241 aaacatgctg ttatggataa atgacgcgct gatggcggta tttttcctgt tagtcggtct
      301 ggaagttaaa cgtgaactga tgcaaggatc gctagccagc ttacgccagg ccgcatttcc
      361 agttatcgcc gctattggtg ggatgattgt gccggcatta ctctatctgg cctttaacta
      421 tgccgatccg attacccgcg aagggtgggc gatcccggcg gctactgaca ttgcctttgc
      481 acttggtgtg ctggcgctat tgggaagtcg tgttccgtta gcgctgaaga tctttttgat
      541 ggctctggct attatcgacg atcttggggc catcattatc atcgcattgt tctacactaa
      601 tgacttatcg atggcctctc ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt
      661 gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctg gttggcgtag tgttgtggac
      721 tgcagtgttg aaatcggggg ttcacgcaac cctggcgggg gtaattgtcg gcttctttat
      781 tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga ctggagcatg tgttgcaccc
      841 gtgggtggcg tatctgattt taccactgtt tgcatttgct aatgctggcg tttcactgca
      901 aggcgtcacg ctggatggct tgacctccat tctgccattg gggatcatcg ctggcttgct
      961 gattggcaaa ccgctgggga ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc
     1021 gcatctgcct gagggaacga cttatcagca aattatggct gtggggatcc tgtgcggtat
     1081 cggttttact atgtctatct ttattgccag cctggccttt ggtagcgtag atccagagct
     1141 gattaactgg gcgaaactcg gtatcctggt cggttctatc tcttcggcgg taattggata
     1201 cagctggtta cgcgttcgtt tgcgtccatc agtttgacag gacggtttac cggggagcca
     1261 taaacggctc ccttttcatt gttatcaggg agagaaatga gcatgtctca tatcaattac
     1321 aaccacttgt attacttctg gcatgtttat aaagaaggtt ctgtggttgg cgcagcggag
     1381 gcgctttatt taacaccaca aaccattacc ggacagattc gggcgctgga agagcgcctg
     1441 caaggcaaat tatttaagcg caagggacgt ggtctcgagc ccagcgagct gggggagctg
     1501 gtctatcgct atgccgataa aatgttcacc ttgagccagg aaatgctgga tatcgtgaac
     1561 tatcgcaaag aatccaattt attgtttgac gttggcgtgg ctgatgcact ttccaaacgt
     1621 ctggtcagta gcgtgctgaa tgctgcagta gtagaaggcg agccgattca tcttcgctgc
     1681 ttcgaatcca cccacgaaat gctgctggag caattaagcc agcataaact ggatatgatc
     1741 atttccgact gtccgataga ttctacgcag caggaaggcc tgttctccgt gagaattggc
     1801 gaatgtggcg tgagtttctg gtgtacaaat ccaccgccgg aaaaaccgtt cccagcttgt
     1861 cttgaagaac ggcggctttt gattcctggg cgacgttcaa tgttagggcg caaattactt
     1921 aactggttta actcccaggg attaaacgta gaaatccttg gcgagtttga tgatgccgct
     1981 ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg ttgccccaac gctttatgca
     2041 tatgactttt atgccgataa aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa
     2101 gagtaccatg ctatttttgc tgagcggatg attcagcacc cggcggtaca gcgaatctgc
     2161 aatacggatt attctgcgct ttttagtcca gcggtgcgtt aatcggcagc tcccccaaag
     2221 ttaaggtggg ggagatagat tagttgtaca ttaccacgat tttgactcgg ctcattattt
     2281 gcccgcttga gacattgttt ccatatgtac gcgggcgaat aaatagagga atctgattac
     2341 ttccttcatg gggatgctga aaagagtagt aattgctttt accatactgg atttcattcg
     2401 taccatcatt ggagagttga aagcctactt gacccgcacc gtcctcactg ttttctaact
     2461 cattttttaa tatgctggtt cctgactcct gtatcgtcac tggttcaaag taatattcaa
     2521 catcactggg ttcaaaaact aattcacctt gctcgccctg gatgcagtca aatcttatat
     2581 gatgaaactg ataattttca ttattgatac gagacagtac aaatcgtcca gtgtcgatgg
     2641 attgatcttc aaaaccacag gtcattaaat ttacctgtaa aatagtattt aaattaaaat
     2701 taaagcggac gctattacaa ttagtgttat tccctgtaca tgaactcact tcaatttcga
     2761 acaacaaagg aaaaggagtt cctggaaaaa tatctgcggt gttggaacta tctgatacag
     2821 atgcgataga gtatggaatg gtgaaattta tcgcagatag catctcttct aaattataag
     2881 atgacttccc tggaagctct atttttttac cgtcagattc aaaggggaaa ttaataatat
     2941 aatgacgact tccgacttta aaccttaaag cgttctgtgt taaaaaatga actttaatgg
     3001 cgttggtcgt ctcttgcaag tctgcacact caatgatatt tcctgtgttt tcaatttttt
     3061 cggcggtgag attacctttc atactcagtg gtttgagtat atatgttcca cgttgttctc
     3121 cgacattggt caatccacag ggagcacttt gcacaataaa agaatataga ctcaaggtga
     3181 ttaataaaag ccacttcata gtaaactcct tttactggca tatatatgtt ttgttttcat
     3241 cgataacgtg ttgaaaagta atagtacaga ataaattctg ttcatcattg agagcaactt
     3301 ttatgccagt tggaatttca tctacgcgaa taaaaaggcg actaccctgg ccaacgactc
     3361 caatattgtt ttcctggaga tccagtacat catagccaaa agttaatggc gaaccatcgg
     3421 gacgcagtgc ctgtatatac cagggcttac gttggtcagt tgtaaactgc acataagaaa
     3481 ctgctccacg gtaaggcgca ataatttgac gattgccctg caattctgtt tcactttcac
     3541 tgtttgctgt atcaagtacc agatggtttt cacgatgagg tatcatcagg tcgtaaacaa
     3601 cctggccttt gttgtttgtt cggttgtatt tttgtccatt aatatgcgcg ccttctaacc
     3661 caggcgcatc cagaattgca aatgtatcgg atagctggtt agtgatatta ataccaccgg
     3721 gccagacaac taatccacta ctaatacttc caccactttg ccaggcgttt ttagaatggc
     3781 tatagctgcc atctattgtg gcgattggag ttctccagct taaattcgta ccaagcgagg
     3841 tatcattatt ttgttgttgc tgattaacat atataccata atttaactga tcatgttcac
     3901 cggctatgcc agtaattcca gtattgttgg aggaatagcc atctttggaa aatgaggtag
     3961 aattcgataa gttaatttgg tgacgtgttt tggcaatatc atcaccccag tagaaaggaa
     4021 tggagataaa cagattaaaa cgctcctctt ctttatcgtt ttcatcatag gattggctgg
     4081 cagagaaagt ataactaatg tgttgccagt tattggagta actgaattgg taatctttag
     4141 catttccact tcgcccccag taattccgcc aaagtgcact taatgataca tttcccagat
     4201 tattggataa aggttgcatt atattggcag aaaggctatt ttttctgcca atatcataaa
     4261 aatcatcata gtcactctga tgatttattt tatcattttc gtaaagatgg tcgctgaatg
     4321 tcctgtaatc ctgcgaagca taacgccagg cggccacgct aaagtgagtg gccgtttgca
     4381 atagatattt attataggcg acctgatagc tcgtaccttc gtgtctggta tcattattta
     4441 acttactact cgatcgggta gcgtcaaaag agatggcacc cagcggtgta ttccaaccat
     4501 ttcccagagt tatagcatta tagttatcgg ataaaatcgt accgccataa agtgtcaaaa
     4561 gattgttgag gccatatata taatttgcct cgagaaagtc ctcctgattc ttgacaccat
     4621 atatctgact acgtccggcg ataaaatcga aatttgaaat accaggttgc agcatattgg
     4681 gcacggaaga gtagggcacc aaaaaggaac gaacattacc atctgcttcc ttaatgctga
     4741 catcgagatc agagccgctg cccgaaagtt gcaaatctgc gatagtaaat ggtcctgggg
     4801 gaacctcttt ttggtaaatg gtatagccat tttgcgaaac cgttatcaag gcgttacttt
     4861 gcgcaacacc ttgtactaaa ggtgtaaagc tttgcataga atcaggtagc atttgcatat
     4921 cgcgaaataa gcgtattccg ctaaatcgca gagagtcgaa tatgagtgag gatgtgtaat
     4981 tttctcctat ctgaactgtg cttaatatct gcgaccagcc acgttccagg taaagagtat
     5041 tactttgcca tttgcctttc atatcatcag gtttgctata actggcatca gagtgtaact
     5101 gccaactaaa taaatttaat ccgctgttaa accggccata actggctgta tgactgttat
     5161 tattgctgtc ataagagcga tactgactta aattatagga ggtataaaaa gcatcaatgc
     5221 cgcgatccca actttctggg ggaacgtagc ctctttcaag ttcattgata tacgcctgtg
     5281 gtactgttaa atttagtcgg tgttcactga tatcccactg atattgtcca ccatgaaccg
     5341 cttcatccag taaaatacaa ttatcttctg tattgaggtt accggtcttt acccccagtt
     5401 tggttagtag caccttagtc agacatggtt tgactggtga ctctgggatc gtgatatctt
     5461 gttttccacg ccactggtta tttacataaa aatcaagttc gtagtttcct ggaagcggtt
     5521 gtttattatc gaaatgatat tcggaaactt tttctccttt catgccgccg atcataaagt
     5581 gcgtatcaaa agtttcttca gcataacccg gaaaagaata caaaaaaata atagcagcga
     5641 cccatcgtgt cgtccttaac atgacttttt cctgttatct cgcggcgatt ttactatgga
     5701 tgttattccc gtaatcatca actaccgtta gttcatattc tgatgcgtgg ttatttttta
     5761 atgtaatgtc gttatttgaa aatggaggca gtaagatact ttcattatta attttcacgt
     5821 tttgtgcttt gatagttgta accgttatc
//
LOCUS       DAPGRN010000202         5759 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPGRN010000202 DAPGRN010000000
VERSION     DAPGRN010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.204"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            16..558
                     /gene="fimA"
                     /locus_tag="RHW33_004536"
     CDS             16..558
                     /gene="fimA"
                     /locus_tag="RHW33_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1647963.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            778..1470
                     /gene="fimC"
                     /locus_tag="RHW33_004537"
     CDS             778..1470
                     /gene="fimC"
                     /locus_tag="RHW33_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV1647964.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            1501..4110
                     /locus_tag="RHW33_004538"
     CDS             1501..4110
                     /locus_tag="RHW33_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV1647965.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGIGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            4123..5130
                     /gene="fimH"
                     /locus_tag="RHW33_004539"
     CDS             4123..5130
                     /gene="fimH"
                     /locus_tag="RHW33_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV1647966.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            5141..5656
                     /gene="sfmF"
                     /locus_tag="RHW33_004540"
     CDS             5141..5656
                     /gene="sfmF"
                     /locus_tag="RHW33_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV1647967.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADNDKTVDLGRWPTTQLLTAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(5659..>5759)
                     /locus_tag="RHW33_004541"
     CDS             complement(5659..>5759)
                     /locus_tag="RHW33_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV1647968.1"
                     /translation="KTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
BASE COUNT         1515 a         1242 c         1385 g         1617 t
ORIGIN      
        1 attgaaggaa tatatatgaa attaagattt atttcgtctg cgctggctgc cgcactattc
       61 gccgctacgg gtagttatgc tgccgttgta gatggcggta caattcactt tgaaggcgaa
      121 ctggtgaatg ctgcctgttc agtgaatact gactcggcag accaggttgt cacactcggt
      181 caatatcgta ccgatatttt caatgctgtt ggtaatacct ctgcattaat tccattcact
      241 atacagttga acgactgcga tcctgttgtt gccgctaatg ctgccgttgc attttctggt
      301 caggctgatg caatcaatga taatttattg gccattgcat ccagtaccaa tacaacaaca
      361 gcaacgggtg tcggtattga aatacttgat aatacatccg caattctcaa acctgatggg
      421 aatagcttct caaccaacca gaacttgatc cccgggacca acgttcttca tttttctgca
      481 cgttataaag gcaccggtac aagtgcatca gcagggcaag caaatgctga cgcgactttt
      541 attatgagat acgaataatc aaaaccacgt tgttttgaat tatatatcac gtcttataac
      601 aaagtaatgt accggttgtc tgaagcggta tggtggcaat gtaaatcgaa atcatgttca
      661 ctttgtatca tgccgcttta ttaaatgaaa agggaatgat gtgttgtaag aaaccaaagc
      721 aatcatttct ttatatttct tatttttgcc gtcaggaata cacaaggcgt attaactatg
      781 atgactaaaa taaagttatt gatgctcatt atattttatt taatcatttc ggccagcgcc
      841 catgctgccg gagggatcgc attaggtgcc acgcgtatta tttatcccgc tgatgctaaa
      901 cagactgcgg tatggattag aaatagccat accaatgagc gttttctggt caattcgtgg
      961 attgaaaaca gcagcggtgt aaaagaaaag tcattcatca ttacaccgcc actatttgtt
     1021 agtgaaccca aaagcgaaaa tactttgcgt attatttaca ccggtccacc gctggcggca
     1081 gatcgtgagt ctctgttctg gatgaatgtt aagacgatcc cttcggtaga taaaaatgca
     1141 ttgaacggta ggaatgtttt gcaactggcg attttatcgc gcatgaagtt atttctccgt
     1201 ccaattcaat tacaagaatt acccgcagaa gcgccggaca cactcaagtt ttcgcgatcc
     1261 ggtaactata tcaatgttca taatccatca cctttttatg tcaccctggt taacttacaa
     1321 gtgggcagcc aaaagttggg gaatgctatg gctgcaccca gagttaattc acaaattccc
     1381 ttaccctcag gagtgcaggg aaagctgaaa tttcagaccg ttaatgatta tggttcagta
     1441 accccggtca gagaagtgaa cttaaactaa ccgaatcatc tgacaatatc agagctaatt
     1501 atgaaaatac ccactactac ggatattccg cagaggtata cctggtgtct ggccggaatt
     1561 tgttattcat ctcttgccat tttaccctcc tttttaagct atgcggaaag ttatttcaac
     1621 ccggcatttt tattagagaa tggcacatcc gttgctgatt tatcgcgctt tgagagaggt
     1681 aatcatcaac ctgcgggcgt gtatcgggtg gatctctggc gtaatgatga gttcattggt
     1741 tcacaggata tcgtatttga atcgacaaca gaaaatacag gtgataaatc aggtgggtta
     1801 atgccctgtt ttaaccaggt actccttgaa cgaattggcc ttaatagcag tgcatttccc
     1861 gagttagccc agcagcaaaa caataaatgc atcaatttac tgaaagctgt acctgatgcc
     1921 acaattaact ttgattttgc agcgatgcgc ctgaacatca ctattcctca gatagcgttg
     1981 ttgagtagcg ctcacggtta cattccgcct gaagagtggg atgaaggtat tcctgcttta
     2041 ctcctgaatt ataatttcac cggtaacaga ggtaatggta acgatagcta tttttttagt
     2101 gagctcagcg ggattaatat tggcccgtgg cgtttacgca acaatggttc ctggaactat
     2161 tttcgcggaa atggatatca ttcagaacag tggaataata ttggcacctg ggtacagcgc
     2221 gccattattc cgctgaaaag tgaactggta atgggagacg gcaatacagg aagtgatatt
     2281 ttcgatggtg ttggatttcg tggtatacgg ctttactctt ccgataatat gtatcctgat
     2341 agccagcaag ggtttgctcc aacagtacgt gggattgccc gtacggcggc ccagctaacg
     2401 attcggcaaa atggttttat tatctatcaa agctatgttt cccccggcgc ttttgaaatt
     2461 acagatttgc acccgacatc ttcaaatggc gatctggacg tcaccatcga cgagcgcgat
     2521 ggtaatcagc agaattacac aattccgtat tcaacagtgc caattttaca acgcgaaggg
     2581 cgtttcaaat ttgacctgac ggcgggcgat tttcgtagcg gtaatagtca acaatcatcg
     2641 cctttctttt ttcagggcac ggcactcggc ggtttaccac aggaatttac tgcctacggc
     2701 gggacgcaat tatctgccaa ttacaccgcc tttttattag ggctggggcg caacctcggg
     2761 aactggggcg cagtgtcgct ggatgtgacc catgcgcgca gccagttagc cgacgacagt
     2821 cgtcatgagg gggattccat tcgcttcctc tatgcgaaat cgatgaacac cttcggcacc
     2881 aattttcagt taatgggtta ccgctattcg acacaaggtt tttataccct tgatgatgtt
     2941 gcgtatcgtc gaatggaggg gtacgaatat gattacgatt acgacggtga gcatcgggat
     3001 gaaccgataa tcgtgaatta ccacaattta cgctttagcc gtaaagaccg tttgcagtta
     3061 aatatttcac aatcacttaa tgactttggc tcgctttata tttctggtac ccatcaaaaa
     3121 tactggaata cttcggattc agatacgtgg tatcaggtgg ggtataccag cagctgggtt
     3181 ggcatcagtt attcgctctc attttcgtgg aatgaatctg tagggatccc cgataacgaa
     3241 cgtattgtcg gacttaatgt ttcagtgcct ttcaatgttt tgaccaaacg tcgctacacc
     3301 cgggaaaatg cgctcgaccg cgcttatgcc tcctttaacg ctaaccgtaa cagcaacggg
     3361 caaaatagct ggctggcagg cgttggtggg accttactgg aaggccacaa cctgagttat
     3421 cacgtaagcc agggcgatac ttcgaataat gggtacacgg gcagcgccac ggcaaactgg
     3481 caggccgctt acggtacgct ggggatcggg tataactacg accgcgatca acatgacgtt
     3541 aactggcagc tgtctggcgg tgtggtcggg catgaaaatg gcataacgct gagccagcct
     3601 ttaggggata ccaatgtttt gattaaagcg cctggcgcag gcggtgtacg cattgaaaat
     3661 caaactggca ttttaaccga ctggcgcggc tatgcggtga tgccgtatgc cacggtttat
     3721 cggtataacc gtatcgcgct tgataccaat acgatgggga attccatcga tgttgaaaaa
     3781 aatattagca gcgttgtgcc gacgcaaggc gcgttagttc gtgccaattt tgatacccgc
     3841 ataggcgtgc gggcgctcat taccgttacc cagggcggaa aaccggtgcc gtttggatca
     3901 ctggtacggg aaaacagtac cggaataacc agtatggtgg gtgatgacgg gcaagtttat
     3961 ttaagtggtg cgccattgtc tggtgaatta ctggttcagt ggggagacgg cgcgaactca
     4021 cgctgcattg cgcactatgt attgccgaag caaagcttac agcaagccgt cacagttatt
     4081 tcggcagttt gcacacatcc tggctcataa aggaaattat caatgaagat aatctgcaga
     4141 ttattattgg cgatggcatg tttgtgtctg gcaaacatat cctgggctac tgtttgtgca
     4201 aatagtactg gcgtagcaga agatgagcac tatgatctct caaatgtctt taatagcacc
     4261 aataaccagc cagggcagat tgttgtttta ccggaaaaat ccggctgggt aggtgtgtca
     4321 gcaatttgcc cacccggcac gctggtgaat tatacatacc gtagttatgt cactaacttt
     4381 attgttcagg aaactatcga taattataaa tatatgcaat taaatgatta tctattaggt
     4441 gcgatgagtc tggttgatag tgtgatggat attcagttcc ccccgcaaaa ttatattcgg
     4501 atgggaacag atcctaacgt ttcgcaaaac cttccgttcg gggtgatgga ttctcgtttg
     4561 atatttcgtt taaaggttat tcggcccttt attaacatgg tggagatccc cagacaggtg
     4621 atgtttaccg tgtatgtgac atcaacgcct tacgatccgt tggttacacc tgtttatacc
     4681 attagttttg gtggccgggt tgaagtaccg caaaactgcg aattaaatgc cgggcagatt
     4741 gttgaatttg attttggtga tatcggcgca tcgttattta gtgcggcagg gccgggtaat
     4801 cgacctgctg gtgtcatgcc gcaaaccaag agcattgcgg tcaaatgtac gaatgttgct
     4861 gcgcaggctt atttaacaat gcgtctggaa gccagtgccg tttctggtca ggcgatggtg
     4921 tcggacaatc aggatttagg ttttattgtc gccgatcaga acgatacgcc gatcacgcct
     4981 aacgatctca atagcgttat tcctttccgt ctggatgcag ctgcggcagc caatgtcaca
     5041 cttcgcgcct ggcctatcag tattaccggt caaaaaccga ccgaagggcc gtttagcgcg
     5101 ctggggtatt tacgcgtcga ttatcaatga ggtacggaga atgagaagag tactctttag
     5161 ctgtttctgc gggttactgt ggagttccag tggatgggca gctgaccctt taggaacgat
     5221 taatatcaat ttgcacggta acgtggttga tttctcttgt accgtaaaca cagcggataa
     5281 tgataagacg gtagatttag gcagatggcc tacgacacaa ctactgaccg ctggcgatac
     5341 cacggcactc gtccctttta gcctgcggct ggagggatgt cctccgggtt cagttgcgat
     5401 tttatttacg ggaacgccgg catccgatac caacctgctg gctctggatg atcccgcaat
     5461 ggcacaaacc gtcgccatcg aattacgtaa tagcgatcgc tcccggctcg cactggggga
     5521 ggcgagcccg actgaggaag tagatgcaaa tggcaatgtc acactaaact tttttgccaa
     5581 ttatcgagcg ttagccagcg gtgttcggcc aggtgtggcg aaagcggatg cgatatttat
     5641 gatcaattat aattaatatt atattaattc gtataatttg gcgtagtcga taagctctac
     5701 aattgaatgc aaacctagct tgccataaat attagattta tgcgcactaa ctgttttat
//
LOCUS       DAPGRN010000203         5720 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPGRN010000203 DAPGRN010000000
VERSION     DAPGRN010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.205"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1034)
                     /gene="umuC"
                     /locus_tag="RHW33_004542"
     CDS             complement(<1..1034)
                     /gene="umuC"
                     /locus_tag="RHW33_004542"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
                     /protein_id="HDV1647969.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGHRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAG"
     gene            complement(1034..1471)
                     /gene="umuD"
                     /locus_tag="RHW33_004543"
     CDS             complement(1034..1471)
                     /gene="umuD"
                     /locus_tag="RHW33_004543"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV1647970.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1468..1716)
                     /locus_tag="RHW33_004544"
     CDS             complement(1468..1716)
                     /locus_tag="RHW33_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HDV1647971.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            2134..3036
                     /locus_tag="RHW33_004545"
     CDS             2134..3036
                     /locus_tag="RHW33_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV1647972.1"
                     /translation="MYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGSEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNCIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            3033..3344
                     /locus_tag="RHW33_004546"
     CDS             3033..3344
                     /locus_tag="RHW33_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647973.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRT
                     R"
     gene            3421..4104
                     /locus_tag="RHW33_004547"
     CDS             3421..4104
                     /locus_tag="RHW33_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV1647974.1"
                     /translation="MSRFVLGNCIDVMARIPDNGIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYVLAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            4105..4326
                     /locus_tag="RHW33_004548"
     CDS             4105..4326
                     /locus_tag="RHW33_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647975.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            4338..4772
                     /locus_tag="RHW33_004549"
     CDS             4338..4772
                     /locus_tag="RHW33_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV1647976.1"
                     /translation="MYGTCEILCRELAAKYPADTPLMLVVWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRIESGISSGVAMEIISNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVINQARTLLKNNTHENN"
     gene            4823..5593
                     /locus_tag="RHW33_004550"
     CDS             4823..5593
                     /locus_tag="RHW33_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647977.1"
                     /translation="MNKTLNALVCRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQRLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVHDAVRSICYQVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSCGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARTEIRSMADALAKIRK"
BASE COUNT         1202 a         1556 c         1649 g         1313 t
ORIGIN      
        1 cctgccttca tatagcggta cccttcacgc cagacatggt ttaaggctct gcatgcggcg
       61 gcaataatgt cccggctgtc ctgtgtgggc aatggaagct tttccacagc ggcattgctg
      121 taacagggtt cttttactgc aaagggtgat gtccgtacaa atgtcgtcac ctgccggcaa
      181 tactgacgct ccccacgtag tttctctgcg gcccgctctg catactgaac aacagcctgg
      241 tgcatggcat ctttgtctgt gattcgttca ccaaaactgc gactacagac aatctgctgt
      301 tttgccggtg gtgcttcttc cagggatatg caggactcgc cgttgagttc gcgtaccgta
      361 cgctcaagaa tgacgctgaa gtttttccgg atgaatgccg tgttagcctg cgccagctgc
      421 agtgctgtgt taatacccag cgcattcagc ttttccgtca gtctgtgtcc tactccccag
      481 acctcaccaa ctggctgcag ccccagtagc ttcaagatcc gattacggtt ttctgccgtc
      541 agcgcgacca ctccggaaaa ctgtggccat tgctttgttg cccactgtgc acttttagcc
      601 agcgtttttg taggcgcaat gcccaccccc atggtgagtc ctgtccagct ctttacctgt
      661 tccctgagct gatgaccaaa aaactccgga gagatgcaat gatttatccc ccgcaaatca
      721 atgaacattt catcaattga gtagggctca actgcgggag aaagcgactc cagaacagcc
      781 ataacccgtt ggctcatgct gtggtacagc gcataattgc tggaaaatac atgtattttc
      841 ttctccaggc gcatttgtct cacctgaaac cagggctgcc ccattctgat gccaagggct
      901 tttgcctccg ggctgcgcgc gatcacacag ccatcgttat tgctgagtac gatgaccggt
      961 tcgttgcgaa ggtccgggcg gaaaactttt tcacatgagg cgtagaaact gttgatatca
     1021 gccagtgcaa acatcagcgt aactcccgcg tcctgtgtat cacgtgagtg acaacaccaa
     1081 aaatacagat gttttccgga tacagtgtgc ggaactccgg gctgtctgaa accggctcca
     1141 gtgccgggcg tgggcgcaac agcagtcgtt tgacggtgaa ctcaccgtcg atctcagcga
     1201 taacgatgtc cccgtgttgt ggtttttcgg ccctgtccac taccagcaga tcaccattct
     1261 gcacgccagc ctggttcatc gattcaccgc tggcgcgcag aaagaaggtg gctgcaggtc
     1321 tgctgatgca atagctgttc agatccagtt cctgctcagc ataatcagtg gcaggcgaag
     1381 gaaaaccggc ctggcaacga tcggcaaaca acggtcgcac gtaactgtca tcgcctgaag
     1441 ggtcagccgg acggtgataa acggtactca tgctgcaggc agccagctgt cgtcctgcca
     1501 gatttcttcc agcagagaca ggatacgttc tttatccttg tcgttgcggg ctcctgtgac
     1561 agatacagac ggggcgctgc cctggtctat acgcagccag atttccgggt agtgtggttt
     1621 cagacgttgt tcgatttcat tctgtagcgc ctgaagtgtg cctgatttga ggttttttgt
     1681 gctctggcgg tcgaaaagaa tttcaatgcg gatcatgttc tcatctccct tttcttttgc
     1741 tgtatgtata tacagtattc taataactga atccgcacac agtcaaatgt cattgttgtg
     1801 actaaaaaac gctcaattct ctgagtgtgt ggctgtatgc ctgctgtggg ctgctgtgtc
     1861 cagcttatcc acagcatttt gtgcacggtt ctgtggacaa aatacctggt tacccaggcc
     1921 gtgccggcac gttaaccggg ctgcatccga tgcaagtgtg tcgctgtcgc cggcctcctc
     1981 acccggtcac gtttcgtcgt tcctcctcca cgcgctgcgg cttcggggcc gcacctgcat
     2041 tcgtatgcgg tcgcccggta acaggtgcgg cacggcctga tggaggccgc atgtgagagg
     2101 agaattccca tgccaaactg gtgctcgaat cgtatgtatt tttctggtga accggcacag
     2161 atcgctgaga ttaaacgact ggccagcggt gcagttacac cgctttatcg ccgcgccaca
     2221 aatgaaggta ttcagctgtt tctggccgga agtgccggac ttctgcagat cactgaaaat
     2281 atccgctctg aacagtgccc cggagtgacg gctgccggac gtggtgctgt atcgacggaa
     2341 aatatcgcgt tcacccgctg gctgacacac ctgcagaacg gtgtactgct ggatgagcaa
     2401 aactgcctga tgctgcatga actctggctg cagagcggta ctggacagcg tcgctgggaa
     2461 ggattaccgg atgatgtcag ggaaaccatc actgtgcatt tcaccgcaaa aagaggtgac
     2521 tggtgtgaca tctggggtag cgaggatgta tcggtgtggt ggaaccgtct gtgtgacaac
     2581 gtagtgccgg aaaaaaccat gccgtttgac ctgctgacgg ttctgccgac ccgcctggat
     2641 gttgaagtga atggctttaa tggtggtgtt ctgaacggtg ttccttctgc atatcactgg
     2701 tatacagaac ggtatggcgt gaagtggcct tgtgggtatg acctgaatat cagtagtcag
     2761 ggagacaact gcattcaggt ggatttcgac acgccgtggt gtcagccgga aagcgacgtt
     2821 gttgcagcat taagccgccg tttcggctgc acgctggagc actggtacgc cgaacagggc
     2881 tgcaatttct gtggctggca gttgtatgag cgcggagagc tcgttgatgt gctctggggg
     2941 gaactggaat ggtcttcccc gacagatgac gatgagctgc cggaagtcac cggacctgcg
     3001 tggatagtcg acaaggtggc gcattacggg ggatgaagta tgacggagac gggcgggcaa
     3061 ccgcccgttt cttttccgac aaaggatgtc gccgtgctcc tctgtttact gcgccggctg
     3121 atgcggcgcg gcataaacgc tgcctgtggt cagtgtcccg cgtccggcgg gcacgggacg
     3181 ggcgctttta ccgctcccgg ctggcgtcgg tgacagtgta cgccagcctg tcgccctttt
     3241 ctgatgaacg cccttcaagc cgctttcgcg gcatattctc gccgtcaaaa agacgacggc
     3301 tgcgatattc cacggtcggc ctgacccgtt accggacgcg gtgaacagcc cacaggcagc
     3361 ggggaacggg caccacaggg gtgccctccc ggtgccctct gagaaagaag gagtgttgtt
     3421 atgtcccgtt ttgtcctcgg taactgcatt gatgttatgg cgcgtattcc tgataacggc
     3481 attgatttca tactgactga cccgccatac ctcgtcggtt ttcgtgaccg acaggggcgt
     3541 accatcgccg gcgataaaac cgatgaatgg ctgcaaccag cctgtaatga aatgtatcgc
     3601 gtactcaaaa aagatgcgct gatggtcagc ttctacggct ggaaccgcgt cgatcgcttt
     3661 atggccgcct ggaaaaatgc gggattcagt gtggttgggc acctggtctt caccaaaaac
     3721 tacacatcga aggccgcata tgtgggttat cgccacgaat gcgcctacgt tctggcaaaa
     3781 ggccgtccgg cgctgcctca gaagccatta cccgacgtgc tgggctggaa atattcaggc
     3841 aatcgtcatc acccgacgga aaagcctgtt accagcctgc aaccactgat tgagagcttc
     3901 acacatccga acgcgattgt gctggacccg tttgcaggca gcggctcaac ctgcgtggct
     3961 gcccttcagt ccggacgccg gtatatcggt atcgagctgc ttgagcagta tcaccgtgcc
     4021 ggacagcaac gtctggctgc cgtacagcgg gccatgcagc agggggccgc aaatgatgac
     4081 tggtttatgc cggaggctgc gtaaatgaac tatgccggac atgaaaaact tcgcgccgaa
     4141 gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga gatggagcgg
     4201 cggtacagct ttaatgctga caccctgccg gaacgtctgg tgcgtcagac gttgtttcgc
     4261 gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggattc ctgcttcaaa
     4321 gattgagggg agagagtatg tacggaacat gcgaaatact ctgccgggag ctggcagcaa
     4381 agtatccggc agacacgcca ctgatgctgg ttgtctggtc ccctgaagag attcaggccc
     4441 tcgctgacgg aatggatatt tccctgtccg atcatgaaat caggaccgtc ctggcgcgcc
     4501 tggaggacat cccggaagac cagcggattg agtccggtat ttcttccggc gtggcgatgg
     4561 agattatcag caacgtgaga gaaaaccgcc aggtgaccgt ccctgctgaa ctgctggcgt
     4621 ccctgattca gaccgctgaa caggcactgt ggaaacgtga atgggccgcc cgtgataacg
     4681 gccttgccgt cccggaatgt gttacccgcc gtcaggcagt gattaatcag gcccgcaccc
     4741 tgctgaaaaa caacacacac gaaaacaact gatgttatcg ccgcctgccc tgcgggcggc
     4801 gatacaggga gaacgcttaa tcatgaacaa aacgctgaat gcactggttt gtcgtcacgc
     4861 ccgtaacctg ctgctggcgc agggctggcc ggaagagacg gatgttgacc agcgaaatcc
     4921 gaactatccg ggctggatca gcatttacgt gcggctggat gcgccccggc tggcgacgtt
     4981 acttatcaac cgtcacggcg gcgtactgcc gccgctcctg gcctccgcca ttcagagact
     5041 gaccggaacc ggggcggaac tggtactgtc cggcagtcag tggcagtcgc tgccggtact
     5101 tccggcagac ggaacgcagg tgtctttccc gtatgccgga gaatggctga ccgaagacga
     5161 aatcagggct gttcttgatg cggtgcacga tgcggtacga agcatctgtt accaggtggc
     5221 agaagatgcg cggcgtatcc gtgcggcgct gaccaccacc ggtcagacgt tgctgacccg
     5281 ccagacgcgc cgctttcgcc tggtcgtgaa ggaaagcgat cacccctgct ggctcgatga
     5341 agatgacgaa aacctgcccg tggtgctcga tgccatcgtg aaccggggag cacgtttttc
     5401 gtcggtggaa atgtacctgg tcagcgattg tattgagcat atcctgtcct gtgggctggc
     5461 ctgcgatgtg ctgcgtatac ccgatgaacc gccccgccgc tggtttgacc gtggtgttct
     5521 gcgggaggtg gtccgggaag cccggaccga aatccgcagc atggcggatg ccctggcaaa
     5581 aatccggaaa tgatgacact ggcggggtaa atccccgcct ttttcctgcc ggtccccgga
     5641 cagagcacaa aggaagtcgc cgccggctcc gctttacccg gccatgcggg gcatggcctt
     5701 gtgggttttc agttatgtgg
//
LOCUS       DAPGRN010000204         5714 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPGRN010000204 DAPGRN010000000
VERSION     DAPGRN010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.206"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            178..888
                     /locus_tag="RHW33_004551"
     CDS             178..888
                     /locus_tag="RHW33_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1647978.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            930..3581
                     /locus_tag="RHW33_004552"
     CDS             930..3581
                     /locus_tag="RHW33_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1647979.1"
                     /translation="MTAFRAAFKAYRLHQVLLLPRFVRLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLVDASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPRFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            3595..4125
                     /locus_tag="RHW33_004553"
     CDS             3595..4125
                     /locus_tag="RHW33_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1647980.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4138..4641
                     /locus_tag="RHW33_004554"
     CDS             4138..4641
                     /locus_tag="RHW33_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV1647981.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPAETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4701..5615
                     /locus_tag="RHW33_004555"
     CDS             4701..5615
                     /locus_tag="RHW33_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1647982.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1636 a         1248 c         1269 g         1561 t
ORIGIN      
        1 tgttattaaa aatatttact ttttagcaag gttttcctat taaaagagaa ccaacattta
       61 ttacaaacgc attaagtctg gaaacccggc gactcgccaa tgtattagca acatttaccg
      121 attgtctgat gtaacggttt tcccgcttat cctcttaaca catggagaat gcatcagatg
      181 caaactacgc gtacccccta ctctatttcc tttatggcga cagtgttact tttattgctc
      241 tttgcttgcc atagcaccgt tgccaacgca gctgtcgcac tgggtgcgac gcgggtaatt
      301 tatccggcaa accagaaaca ggttttattg cctgtcacga ataatgatcc tgccagtgtc
      361 tatctcattc agtcatggat agaaaatgca ggtgaccaaa aagacaccca gtttgtcatt
      421 accccaccgc ttttttccat gcaaggtaaa aaagagaata cgctgcggat aatcaacgcc
      481 actaatcatc agttaccagg cgaccgtgaa agcctgttct gggtgaatgt taaagctatt
      541 cctgctatgg aaaaagacca gaagaatgag aatacattac agctggcaat tattagccgc
      601 ataaaaatgt tttatcgccc cacgaacctg gcaatggcac ctgaagaagc gcctgcaatg
      661 cttcggtttc gtcgcagcgg tagcaaactc actcttatta atccaacgcc ctacttcatt
      721 accgtaacaa atatgaaagc aggaaatagc aatctcccaa acaccatggt gccgccaaaa
      781 ggtgaagtat cggtggatat cccgcatgct gccaccgggg atatcagttt tcagacgatc
      841 aatgattacg gcgcattaac tccgcgtatt aaggcaacca tgcagtaagc cagtgaccta
      901 attaataata aactcaatcg gaattaataa tgacagcctt tcgtgccgca tttaaggcat
      961 atcgtttgca tcaggtgtta ctactgcctc gctttgtacg gcttaccatt gcccttggct
     1021 tagcaactgc ggtatttccc gttgacgcag agtattattt taatccacgg tttttaagca
     1081 atgatcttgc tgaatctgtc gatttatccg cctttactaa gggacgcgag gcaccacctg
     1141 gtacatatcg ggtcgacatt tatttgaacg acgaatttat gactagtcgg gatattactt
     1201 ttattgcaga tgataataat gcagatctca tcccatgcct gagtacagat cttcttgtca
     1261 gtcttggcat aaaaaaatct gcgttattag acaataagga acattcagca gaaaaacatg
     1321 tgccggacaa cagcgcatgc acaccacttc aggatcgttt ggtcgacgca tcaactgagt
     1381 ttgatgttgg tcagcaacat ctgtcactct ccgttccgca aatttatgtt ggcagaatgg
     1441 ctcgcggcta tgtttctcct gatctgtggg aagaaggaat aaatgctggg cttctaaact
     1501 atagttttaa tggaaattct attaataatc gtagtaacca taatgcagga aaatcaaact
     1561 atgcatattt gaatttacag agtggcatca acattggtag ttggcgactg cgcgataact
     1621 caacgtggag ttataacagt gggagtagca attcatctga cagcaataaa tggcagcata
     1681 tcaatacgtc ggctgaacgt gacattattc ccttacgctc acggttaacg gtaggtgata
     1741 gttataccga tggcgatatt tttgatagtg tgaactttcg tggcctcaaa ataaattcaa
     1801 cagaagcgat gttgcccgat agccaacatg gtttcgctcc ggtgattcat ggtattgccc
     1861 gtggcaccgc acaagtgagt gtaaaacaga acggatacga tgtttatcag actactgttc
     1921 cacccggccc ttttactatt gatgatatca actctgcggc caatggtggt gatctgcaag
     1981 taaccataaa agaggcagac ggcagtattc agacattata tgttccttat tcgtctgttc
     2041 cggttctcca acgtgctgga tatacgcgtt atgcgcttgc catgggggaa tatcgtagtg
     2101 gaaataacct gcaaagctcc cccaggttca tacaaggtag cttgatgcat ggactggaag
     2161 gaaactggac accttatggc ggaatgcaaa ttgcagaaga ttatcaggcc ttcaaccttg
     2221 gtattggtaa agatttagga ctttttggtg ccttttcttt cgatatcacg caggccaata
     2281 cgacacttgc agatggcacc cgtcacagcg ggcaatcggt taaatccgtc tacagcaaat
     2341 ccttctacca gacaggaacc aatatccagg tcgcaggata tcgctattct acgcaaggtt
     2401 tttataactt atccgacagt gcctacagtc gaatgagtgg ttacaccgtc aagcctccta
     2461 ccggagacag caatgagcag acacaattta ttgattattt taatctgttc tacagtaagc
     2521 gtggtcagga acaaataagc atctctcagc agcttggaaa ttacggtacg acatttttca
     2581 gtgccagtcg ccaaagttac tggaacacgt cacgcagcga ccagcaaata tcatttggat
     2641 taaatgttcc gtttggtgat attacgactt cactgaatta cagctattcc aataatatat
     2701 ggcaaaacga tcgggatcat ttactcgctt ttacgcttaa tgttcccttc agtcattgga
     2761 tgcgtacaga cagtcagtcg gcatttcgta attcaaacgc cagttacagt atgtcaaacg
     2821 atttgaaagg cggcatgacc aatctatcgg gggtttatgg cactctgctg ccggataata
     2881 acctgaatta tagcgttcag gtcggtaaca cccacggagg taatacatcg tctggcacca
     2941 gtggttacag ttctcttaat tatcgtggag cttacggtaa tactaatgtc ggttacagtc
     3001 ggagtggtga cagcagccag atttattacg gaatgagtgg tgggattatt gctcatgctg
     3061 atggcatcac ctttggacag ccgctgggcg acacaatggt tctggttaag gctcctggcg
     3121 ctgataatgt caaaatagag aaccagaccg gaattcatac cgactggcgt ggctatgcca
     3181 tattaccatt tgcgacagaa tatagagaaa atcgtgtcgc tcttaacgcg aattcccttg
     3241 cagataatgt tgaactggat gaaaccgtag tcactgtcat cccaactcac ggtgctattg
     3301 ccagagcaac atttaatgca caaatcggag ggaaagtatt aatgacgttg aagtacggta
     3361 ataaaagcgt tccattcggt gcaattgtca ctcacggaga gaataaaaat ggcagcattg
     3421 tcgcggaaaa tggtcaggtt tatctgactg gacttccaca gtcagggaaa ttacaggttt
     3481 catggggcaa tgataaaaac tcaaactgta ttgtcgatta caagcttcct gaagtctctc
     3541 ctggaacctt gctgaaccag cagacagcaa tctgtcgcta aggaacaata gattatgaaa
     3601 tacaataacg ttattttcct cggtttatgt ctggggttaa ccacctattc tgctttatcc
     3661 gcagatagcg ttattaaaat tagcgggcgc gtcctcgatt atggctgcac agtctcatcg
     3721 gattcgctta attttaccgt agatctccaa aaaaacagtg ccagacaatt tccaacgacc
     3781 ggtagcacaa gtccagccgt cccttttcag attacgttaa gtgaatgcag caaagggaca
     3841 acgggggttc gggttgcatt taacggtatt gaggatgcag aaaataatac tctgttgaaa
     3901 ctggatgagg gaagcaatac ggcctccggt ttaggtatag aaatactgga cggaaatatg
     3961 cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact ggtaccagaa
     4021 cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc cgtcaatccg
     4081 ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga gtcagatatg
     4141 aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac agcaactctc
     4201 caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc ctgcactatt
     4261 caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct gcaacaacct
     4321 ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc ggctgaaaca
     4381 agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta caaaaatgaa
     4441 ggtactgccg aaaatattca gatagagctg agggatgacc aggatgcgac gttaaaaaat
     4501 ggcgatagca aaacggttat tgttgatgag atcactcgta atgcacagtt tccacttaag
     4561 gcaagagcta tcacggtgaa tggaaacgca agccagggaa cgatcgaggc gctaatcaat
     4621 gtgatctaca cctggcaata acgtcgattc gattttctta gccttctttt taaatcaatt
     4681 ttttaacctt aggatatatc atgggtaaaa caatcagtat aaaggtccta ttcggtattt
     4741 atcttctatt aatggcaggc aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa
     4801 gtattggagc tggtacaacc tctgtttatg ttaatctcga ccctgtaata cagccgggcc
     4861 agaatctggt tgtagacttg tctcagcata taagttgctg gaatgattac ggcggctggt
     4921 acgacactga tcatataaac ctggtacaag gctcagcttt cgcaggctct ctacagtcat
     4981 ataaaggatc gctgtactgg aataatgtga cctacccctt cccgttgacg acaaatacca
     5041 acgtattgga tattggtgat aagacaccga tgccattacc gttaaaactc tatattaccc
     5101 cagtcggggc ggccggtgga gttgtcataa aagccgggga agttattgcc cgtattcata
     5161 tgtataaaat tgccacgtta ggaagcggaa atccgcgtaa ttttacgtgg aatattattt
     5221 caaataacag tgttgttatg ccaacaggcg gctgcactgt tgatagtcgt aatgtcaccg
     5281 tgaatctccc ggactttcct ggaagcgcag aaatccccct tggcgtatat tgctcaagtg
     5341 aacaaaagct ctcattttat ctttcgggtg ctaccactga cagtgctaga caagtgtttg
     5401 ctaacacagc accagatgca acaaaagcat cgggagtggg tgtctctctg atgcgtaatg
     5461 gaaaaatact ggctacggga gaaaatgtct ccctggggac agtaaataaa agcaaagtac
     5521 ctctgggttt aagcgcaacc tatggacaaa cagggaacaa agtttcagcc ggaaccgtcc
     5581 agtcagtcat tggtgtcact tttatctatg aataattgtg attttgacct tagtctatgc
     5641 gctccattca cgaaaattgc gcggataatt attttgtgaa ggctattatc ctacacctgt
     5701 aagaaaatcc gcgc
//
LOCUS       DAPGRN010000205         5652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPGRN010000205 DAPGRN010000000
VERSION     DAPGRN010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.207"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            132..782
                     /gene="csgD"
                     /locus_tag="RHW33_004556"
     CDS             132..782
                     /gene="csgD"
                     /locus_tag="RHW33_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV1647983.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            787..1176
                     /gene="csgE"
                     /locus_tag="RHW33_004557"
     CDS             787..1176
                     /gene="csgE"
                     /locus_tag="RHW33_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV1647984.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1201..1617
                     /gene="csgF"
                     /locus_tag="RHW33_004558"
     CDS             1201..1617
                     /gene="csgF"
                     /locus_tag="RHW33_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV1647985.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1644..2477
                     /gene="csgG"
                     /locus_tag="RHW33_004559"
     CDS             1644..2477
                     /gene="csgG"
                     /locus_tag="RHW33_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV1647986.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2541..3032)
                     /locus_tag="RHW33_004560"
     CDS             complement(2541..3032)
                     /locus_tag="RHW33_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV1647987.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALTPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3134..3688)
                     /gene="ycdY"
                     /locus_tag="RHW33_004561"
     CDS             complement(3134..3688)
                     /gene="ycdY"
                     /locus_tag="RHW33_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV1647988.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3712..4053)
                     /gene="ycdX"
                     /locus_tag="RHW33_004562"
                     /pseudo
     CDS             complement(3712..4053)
                     /gene="ycdX"
                     /locus_tag="RHW33_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
     gene            4093..4362
                     /locus_tag="RHW33_004563"
     CDS             4093..4362
                     /locus_tag="RHW33_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647989.1"
                     /translation="MNITKAIHNYARRRGIELQLEDEQVRFWEIEQDCEWMFSYSIAQ
                     CGCLFWKGNVYLPRDIKEELPAMIGTDKKLKEVLDFIHKEFISKK"
     gene            4670..5083
                     /locus_tag="RHW33_004564"
     CDS             4670..5083
                     /locus_tag="RHW33_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647990.1"
                     /translation="MKKIIAGVVLALGLVGAANANTYYVCNSYFEYKGDDTRYLSDGD
                     RYYHAPSGKYRGFIGVKVRVNDDNSQFAFNDPVYDKTIKSPKLKEDKNEKEEYYGSED
                     GKSYIKYLHSVDGAPIFDVKKGDTSYTLISCREIL"
     gene            5166..>5652
                     /locus_tag="RHW33_004565"
     CDS             5166..>5652
                     /locus_tag="RHW33_004565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647991.1"
                     /translation="MEIRFARDIDWIKNNSRSISPSVFESRIITLCKEINYEFLGFYG
                     EWKGIKTKLVLKCSEGHVYNSATIDGFIGSGRRCPECKKINLVKKKIRAISPEEYTRR
                     LNDVCLEDGYIFIGFYGEWKGNETKVIMRCPYGHEYNTATINSFLCGIRCPQCKRDKL
                     IE"
BASE COUNT         1619 a         1258 c         1271 g         1504 t
ORIGIN      
        1 ttttatattt tacccattta gggctgattt attactacac acagcagtgc aacatctgtc
       61 agtacttctg gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa taaaaaaagc
      121 ggggtttcat catgtttaat gaagtccata gtattcatgg tcatacatta ttgttgatca
      181 ctaaaccttc tttgcaggcg acagctctct tgcagcacct taaacaatcg ctggcaatta
      241 caggaaaatt acataatatt caacgttctc tggacgatat ctcttcaggc tctattattc
      301 ttctggatat gatggaagcg gataaaaagc ttatccatta ttggcaggat actttgagca
      361 ggaaaaacaa caatatcaaa atattgttgc taaatacgcc tgaagattac ccgtaccgcg
      421 acattgaaaa ctggcctcat atcaacggcg ttttttatgc catggaggat caagaacgtg
      481 ttgtcaatgg gttgcaaggc gtcctgcgcg gcgaatgcta ctttacgcaa aagcttgcca
      541 gttacctgat tacacattct ggtaactatc gttataacag cacggaatca gccctcctta
      601 ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg
      661 ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca tctttataat cttttcaaga
      721 agatagccgt aaaaaaccgg acacaagcgg tttcctgggc aaacgataac ctcaggcgat
      781 aaagccatga aacgttattt acgctggatt gtggcggcag aatttctgtt cgccgcaggg
      841 aatcttcacg ccgttgaggt agaagtcccg ggattgttaa ctgaccatac tgtttcatct
      901 attggccatg atttttaccg agcctttagt gataaatggg aaagtgacta tacgggtaac
      961 ttaacgatta atgaaaggcc cagtgcacga tggggaagct ggatcacaat aacggtcaat
     1021 caggacgtta ttttccagac ttttttattc ccgttgaaaa gagacttcga aaaaactgtc
     1081 gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta
     1141 ttaagtacgg gcgatttggc gcatgatgaa ttctaaataa aaaattgttc ggaggctgca
     1201 atgcgtgtca aacatgcagt agttctactc atgcttattt cgccattaag ttgggctgga
     1261 accatgactt tccagttccg taatccaaac tttggtggta acccaaataa tggcgctttt
     1321 ttattaaata gcgctcaggc ccaaaactct tataaagatc cgagctataa cgatgacttt
     1381 ggtattgaaa caccctcagc gttagataac tttactcagg ccatccagtc acaaatttta
     1441 ggtgggctac tgtcgaatat taataccggt aaaccgggcc gcatggtgac caacgattat
     1501 attgttgata ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc
     1561 ggacaaacct cgaccatcca ggtttcgggt ttacaaaata actcaaccga tttttaagcc
     1621 ccagcttcat aaggaaaata atcatgcagc gcttatttct tttggttgcc gtcatgttac
     1681 tgagcggatg cttaaccgcc ccgcctaaag aagccgccag accgacatta atgcctcgtg
     1741 ctcagagcta caaagatttg acccatctgc cagcgccgac gggtaagatc tttgtttcgg
     1801 tatacaacat tcaggacgaa accgggcaat ttaaacccta cccggcaagt aacttctcca
     1861 ctgctgttcc gcaaagcgcc acggcgatgc tggtcacggc attgaaagat tctcgctggt
     1921 ttataccgct ggagcgccag ggcttacaaa acctgcttaa cgagcgcaag attattcgtg
     1981 ctgcacaaga aaacggcacg gttgccatta ataaccgaat cccgctgcaa tctttaacgg
     2041 cggcaaatat catggttgaa ggttcgatta tcggttatga aagcaacgtc aaatcgggcg
     2101 gggttggggc aagatatttt gggatcggtg ccgacacgca ataccagctc gatcagattg
     2161 ccgtgaacct gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg gtgaacacca
     2221 gtaagacgat actttcctat gaagttcagg ccggggtttt ccgctttatt gactaccagc
     2281 gcttgctgga aggggaagtg ggttacacct cgaacgaacc cgttatgctg tgcctgatgt
     2341 cggctatcga aacaggggtc attttcctga ttaatgatgg tattgaccgt ggtctgtggg
     2401 atttgcagaa caaagcagaa cggcagaatg acattctggt gaaataccgc catatgtcgg
     2461 ttccaccgga atcctgacag ataaaaatgc cagaattaat aatccggcat ttactgctta
     2521 agcgaaagcg tgagtgcccc tcacgcttta tttttgattt cctgctcacg gtgcgccgtc
     2581 tttgcactac gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc aaatatcagc
     2641 ccaagcgcca gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa cgtcgcgcat
     2701 gcgcctataa acgtccccgg aacaaatgaa agcagcagct gtttggcctg aatacacatc
     2761 agaaacgcga caataccggt tatgacataa ccgagaattt ccaaatgtgg cgtcagtgca
     2821 ctaccgtaaa taatgaccat cgcccacacc acgccactaa gcagggttgc agcggagatc
     2881 gccagccctt tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc caggaagccc
     2941 gcccagctta gtaagcccag ggaaacagcc acccatcccc agataccgga gagaatgcct
     3001 gttgtgattg caatagagag aagtatgttc atgccgcgca tgatagcaga aaacgcggcc
     3061 atgaatgaga tcgagcacac attttatgca acagcaaatt atctgttgca gtcatgagtg
     3121 acgcagatca cattcactct tcagaatcct cttccagctc gtcccacatt gcactaatgg
     3181 catcacgggt tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg gcctccactt
     3241 tgccaaggaa cgcaccacac cagggtaaca gatactcact gaacagtgtt tccagtgctt
     3301 cgctctcatc ttccgttgac tgatcttcca gccaggaagc cgcaagcagc aatgtgccga
     3361 tgtgatcggc aggcgtatcc gctaatggca tccctcgttc agaaagaaaa gcgcgcactt
     3421 ccgcttccgt tgcgtcctca acccatgcgc tacgatatgg cggcacggca cattcatcgc
     3481 cgataaacaa cgcattgtaa tcggcagaga cttgcgtcat atcacagctt ttctgcaaac
     3541 gtgtcagcaa ctcatcctgc tccagtggcc agttcgcagc cagtttcccc tcacgaatca
     3601 gggtaaacag cggcaccagt aaaggatctt gtggttggcg gtaatagagc gaacccagca
     3661 cacgacagag gatagaaaac tcgttcatta atatatttcc attactagta attaaaggtc
     3721 tgcaaattcc gcaatcggtg ccataccgcg agattcaagg aagttcagta agcggcgcgg
     3781 agaaacattc aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt taagacactc
     3841 ttcaaattcc cccatggtaa acgcggtgtg agaatccgag cctaatgcca cccaacctcc
     3901 agcatcacgt accgctgcgg ctaccgcacg acagttgtct tcactgccct tacgtgagtg
     3961 taaaaatgag gaattattga tttccagcgc cacctgatgt ttcgcggctg cttcagcaac
     4021 ggctttaaca tctatctcat atttcggatt ccctaatatc atgtacccac aataacacga
     4081 gggggtacaa caatgaacat taccaaagca atccataact atgctcgccg ccgtggaatc
     4141 gaacttcaac tcgaagatga gcaggttaga ttctgggaga tagaacaaga ttgtgaatgg
     4201 atgttcagct attcgattgc tcaatgtggt tgcttgttct ggaaaggtaa tgtgtatctt
     4261 ccccgcgata ttaaagaaga acttccggcg atgattggca ccgataagaa gttaaaggaa
     4321 gtcttagatt ttatccacaa agagtttatt agcaagaagt aatttctaat ttggtgggtg
     4381 caattcccac cgctttttaa atggagaatc aaacatgtta gcttttatct cattcgtggg
     4441 aggaagtctc acaactatga ttagtctttc ttatcttgtc gttagtatgg ggtgagcaat
     4501 gaaacaattt catgatggca tttcatctaa gacattcaag aaagaatata gcggattgtg
     4561 tattaactgg attgatttga ttggtgcggt tgtgtttggt ctggtaatca gcatcaactt
     4621 ttaacgatat aaatatatcg taattcaaac aacaagagga aattctacta tgaaaaagat
     4681 tatcgctggt gttgttcttg ctctgggttt ggttggtgct gcgaatgcta atacctatta
     4741 tgtgtgtaat tcctactttg agtataaagg agatgataca cgctacctga gtgatggcga
     4801 ccgttattat catgcaccgt ctggcaaata ccgtggcttt atcggtgtga aagttcgagt
     4861 gaatgatgat aatagtcaat ttgctttcaa cgatccggta tatgataaga caatcaaaag
     4921 cccgaaactg aaagaagata agaacgagaa agaagaatat tatggttccg aagatggtaa
     4981 atcctacatc aaatatctgc attctgttga tggcgcacca atatttgatg ttaaaaaagg
     5041 tgatacatct tataccctga ttagctgccg tgaaattctt taatctttaa tcacctagcc
     5101 cctatcgtta taatacgcgg taggggcgag tgtgtctaat ttactgtgtt aagaggaagc
     5161 caactatgga aattaggttt gcgcgtgata ttgattggat taaaaataat agtcgctcaa
     5221 tatcacctag tgtattcgaa tctcgcataa tcactctgtg taaagaaatt aactatgaat
     5281 ttcttggatt ctatggtgag tggaaaggga ttaaaacaaa acttgttcta aaatgtagtg
     5341 aggggcatgt ttataattct gccacaatcg atggatttat tggttctggg cgtcgctgtc
     5401 ctgagtgcaa aaagattaat cttgtaaaga aaaaaattcg tgcaatctct cccgaagaat
     5461 atacgcgacg tcttaatgat gtatgcctag aagatggtta tatattcatt ggattctatg
     5521 gtgagtggaa aggtaacgag acaaaagtaa tcatgcggtg tccttatggt catgaatata
     5581 acacagccac tataaacagc tttctatgtg gtattcgatg tcctcaatgt aagcgagaca
     5641 agctaataga ac
//
LOCUS       DAPGRN010000206         5596 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPGRN010000206 DAPGRN010000000
VERSION     DAPGRN010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.208"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            313..534
                     /locus_tag="RHW33_004566"
     CDS             313..534
                     /locus_tag="RHW33_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HDV1647992.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSSDVQGVL"
     gene            531..1574
                     /locus_tag="RHW33_004567"
     CDS             531..1574
                     /locus_tag="RHW33_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HDV1647993.1"
                     /translation="MKKPLVTRNDIAEAIALHTACMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEAHRMTSNNLNVKKPASVASATP
                     SPAVNVISNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIKKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPAPSIPQTLPD
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDENGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVAIG"
     gene            1738..2538
                     /locus_tag="RHW33_004568"
     CDS             1738..2538
                     /locus_tag="RHW33_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDV1647994.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            2568..3413
                     /locus_tag="RHW33_004569"
     CDS             2568..3413
                     /locus_tag="RHW33_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein repL"
                     /protein_id="HDV1647995.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRMIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQHAKNALIESKLKISSAATKVKAVLAKTLALFDFLSTPPCQNDTPSP
                     CQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAKADNLRLSKKRAQKHE
                     FKQKVEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALNAVLAKNKDNEKWGIP
                     AGFRG"
     gene            3464..3709
                     /locus_tag="RHW33_004570"
     CDS             3464..3709
                     /locus_tag="RHW33_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1647996.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKERAEEILV
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            3891..4046
                     /gene="hokD"
                     /locus_tag="RHW33_004571"
     CDS             3891..4046
                     /gene="hokD"
                     /locus_tag="RHW33_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV1647997.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(4240..5352)
                     /locus_tag="RHW33_004572"
     CDS             complement(4240..5352)
                     /locus_tag="RHW33_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HDV1647998.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT         1517 a         1392 c         1323 g         1364 t
ORIGIN      
        1 atttttaagt cgcgaatgct atctttttgc atcatattga ccttttaatc gttcaggctt
       61 atagtttcgc cgtcgtagca aattctgcga ccgggtttag cagcctgaat gattgtgcgg
      121 acagccgcag atatccgata ttgcggtatt tttgtgtccg taaaaccgcg ttacgcccaa
      181 attatggtgg ggcgtgatgg ggaggcttcg gcctgctggt ttcacaatcg ccagtctgct
      241 aaccccgtca cgtcctgcca cctgtttagc agcgggtagc aggttgttaa acctgattgt
      301 gaggccgtaa ctatggttaa tgccaatcct tgcgcacgcc aagaattcat ctggcgtttc
      361 tattcctgca aaaaacacca ctatcacttc gtcatcgcag caacagaaga cgaagcacgc
      421 tctcaattgc ctgatggccc ctgcattttt actgcccgtt tttcaactaa ctcgcgcaat
      481 tcacttagtt actggaacct ccccttctct tccgacgttc agggggtttt atgaaaaaac
      541 ctctcgtcac ccgtaatgac atagccgaag cgatcgccct gcatactgcc tgtatgccga
      601 cacgggagat ccccggcgca attgccaact atttcatgat aaccagacgt ttttataccc
      661 gaacagataa ggctgtgatc aacaggctac tgatagccga gatcagggat tatttgattg
      721 aacaaggacg tctacgttac gcaacggtgg cagcagaaat gagaaaggag gcacatagaa
      781 tgaccagtaa taatttgaat gttaaaaaac cagcatctgt tgcttcagct acgccatcac
      841 cagctgtgaa tgttatctcc aacactggag acacaatcga cagccagacg ctgttaaaga
      901 tggtcaatga agcgcgtaag ttatgtgggg aaccagaggt tcggaacaac aaattcatca
      961 aaaaaatact cgacgaatta gaaggtgagg acggttacac aaaaagtgca accgtgccgc
     1021 caggtggcgg tacgcctatg gttgtcataa ccatgaccta caaacaagcc ctgcgagtcg
     1081 ccgcgcgcga gtcaaaagca gtccgccgtt cgctgatcga caaactggaa gaattgcagc
     1141 aggcaaactc ccctgcccca tcgatccccc aaacattacc agacgctcta cgcctggctg
     1201 ccgagttggc agaacagaaa atgcagttgg aacaacagct ggtggccgca gcccctaaag
     1261 tcgattttgc cgaccgggta tcagcggcta atggaatcct gatcgggaac tttgcaaagg
     1321 tcgttggact taagcaaaac gcccttttct catggttgcg ccagaacggc attctcatgg
     1381 cttttggtgc gcgcaaaaac gtaccgcgcc agcagtacat taacgccggg tatttcacgg
     1441 tgaaagaagt ggtactggat gatgaaaatg gctaccagat acggctgacg ccccaattaa
     1501 cgggtaaagg ccagcagtgg ttaactcgca agctacttga tgctggtttg ttaaaaccag
     1561 tagcaatagg ttaacaaaag aaaaaacctg ccagcaaact ggcaggtttc tgagcagatc
     1621 gaccaacccg atctggatcg agtcagaaaa atttgctcta ataaatttcg ttttctaagt
     1681 gcaaagaatc accatttcga gctggtgatt gaaggttgat gcaaatttgg agaaaaaatg
     1741 caacaaacat tcaatgcgga tatgaatata tcaaaccttc atcaaaatgt cgatccttca
     1801 accactctgc ctgttatttg tggtgttgaa attacgaccg accgcgctgg ccgttacaac
     1861 cttaatgctc tacacagagc gagcggactc ggtgcccata aagcgccagc tcaatggcta
     1921 agaacgctgt cagccaaaca gctcatcgaa gagcttgaaa aagaaactat gcagaattgc
     1981 atagtttcgt tcgaaggccg tggcggcggt acgtttgccc atgaattgct cgctgtggag
     2041 tacgcaggct ggatttctcc cgcgtttcgg ctgaaggtaa accagacatt tatcgactat
     2101 cgagccggaa gattgcaacc tgctattccg cagagtctcc ctgaagctct ccgtttggct
     2161 gccgacctgg cagagcaaaa gcaacggctg gagcaaaaaa tgcttatgga tgcgcctaaa
     2221 gtcgaattcg ccgaacgcgt tgctaccgcc agcggggttc taatcggcaa ctatgccaaa
     2281 gtgctcggcc tgggccaaaa ctatctcttc acctggttgc gtgataacgg aattctgatc
     2341 gcaaccggtg aacgcaggaa cgtccccaaa caagaataca tatcccgtgg gtacttcacc
     2401 ctcaaagaaa ccgtgatcga tacaagcaac ggaagcagga tttctttcac gactcgtata
     2461 accggcaaag gtcagcagtg gctaatgaag cgattgcttg atgctggtgt gctggtacct
     2521 gtcgcggcta cgcgctaaca gacgtagtaa gaaccaccag cattgtaatg ctggctaaag
     2581 tcactttcct gagctgtata acgatgagcg attttacttt ttctggctat gaattggcct
     2641 gctttgtaac acactccggt ctatcccgta gcgccgggca tatcctgtcg caatgtgcaa
     2701 atctcgcggc aacaaccagt gaatacttca ttcacaagcc tcaccgcatg atcgcggcag
     2761 aaactggtta tagccaatca accgtcgttc gcgcattccg tgaagctgta aacaaaggaa
     2821 tcctgtcggt agagattgtt atcggcgatc accgtgaacg ccgcgctaac ctgtaccggt
     2881 ttacaccatc ctttttggcc ttcgcacaac acgctaaaaa cgcgctgatt gaaagcaaat
     2941 taaagatctc ttcagccgca accaaggtta aagctgttct cgctaagaca ttggctttat
     3001 ttgatttttt atccacaccc ccatgtcaaa atgatacccc ctccccctgt caggatgacg
     3061 tggcaataaa gaataagaag tcacaagtta aaaaaacaaa aagatcagtt tccggcggtg
     3121 ccggaacgac cagactcaaa aaattgactt catggatcgc tgaggcaaaa gcaaaggctg
     3181 acaatctgcg gttatccaaa aaacgcgctc aaaaacatga gttcaagcag aaagtagagg
     3241 cggctgcgcg gaaatatgct tacctgaaga acaagcgttc gcctgatatt ggcgggatat
     3301 caaacttcga taatctaccg cattgcatga cggtaaacga agctcttaat gcggttttag
     3361 ccaaaaataa agataacgaa aaatggggta taccggcagg attcaggggg tgatagattg
     3421 ctctaatctg gagtcacctg gcgttttcag tttgaggtcg gagatgcaat ctgatttttt
     3481 acagttagcg atcgcttttg caggatatgt ttgtattggc ttctgtgtat acatgatcag
     3541 ccgaaaaatg cttgtcgata tcgaccgcaa agaacgagca gaggagatct tagtatggat
     3601 tttctttggc gcggtctggc cattagggat catgtttgct gcaacatttc ttctgatgtg
     3661 gatattcacc cttccaggtg atttctatag aaaaaaagcc agacattgat acaatcgttg
     3721 cgggtgcttg aggctatctg cttcaggcat gacccgaaaa gcagatagaa gaaagcccca
     3781 gataacatta cgcgtcctgc aagacgctta acattaatct gaggccatat ctatgcttag
     3841 catacgtaga ttagcctctt accgaccaaa aggtcaagga gaagcaggct atgaagcagc
     3901 aaaaagcgat gttaatcgct ctgatcgtca tctgtttaac cgtcattgtg acggcactgg
     3961 taacgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcactgtct
     4021 tcacagccta cgaatctgaa aggtaagaga cctggcgggg agtgatccca taagcgctaa
     4081 cttaagggtt gaaccatctg aagaatgcga cgcctcggtg cctcgttaag acgatgcctc
     4141 gcgttcttca attgcgtttt gtaggctgtc agggatactg tcccacgaat ggccacctgt
     4201 aagctccaga tgaccatttt tgttattctc cacaacgagt tagttcttct tttcggatcc
     4261 ggcacttctg ggggggaaat ccagcgatgg ctggattatg tcgtcaatta aaaatgcggc
     4321 gagtagatta gcaaatatcc acgctttcgc gagttcaggt tcctttgcac gcaaagcatc
     4381 caggtgcagc aaacttttga gccgcttaaa agccagttca atttgccatc gcagacggta
     4441 acaatcagcc acttgctctg ctgaatattc atcttccggt aatgatgtta gcaatagcac
     4501 atggcccgct gcttccagcg tttccgcctg aactactcgt ccttttcgac gattctcgct
     4561 gagcagtcgg gttttactga ttaatgcttt ttcgggagga agtgatacgg caatgagacg
     4621 tgccggaaag ggagctccgg cttttttatt acctgaattg cctatcatta cagtggtttc
     4681 accgttctta ccgcaatcca gcccgcgcag aaaacccatc atgtcaaagc gcattccttc
     4741 tgcagttaac cagcgcaatc ctcgccagtg aacccggacg atataatcag cttctccaaa
     4801 agcaagtgag cggatacatt cgggacgcga accgaatccc cggtcagcaa tgcgtatctc
     4861 gtctgccgtt tgcgcaaatc ggtccagccg ttcagcgtct ctgctgtcgg ttagctcaaa
     4921 atcagtgaac tgacaggtat gaggatcata tcccatatgt agtcgccatt cagcgctgcc
     4981 gcccccgggc gcactgattg ctgttccatc gacaagacgc aatctctttc cgcttgtaca
     5041 acccgtaact gcggcgcgta cagcaagtgt ttgtgcggca agtatgccaa accagtcggc
     5101 ggcattccgc agccgcttca ggagagccac gtcagataat gttgcaacgt catggagctg
     5161 agcccatgca gtgacttcac gtaatgacat ccccccgggg ccgtaagcca gccccagacg
     5221 tagcagagtt gcagcatcac gaatttcgcg gcggcgggtt agagccccgg cattacgtgc
     5281 cgaagtatcc agttcttcgg gcttaccaat atgggccaga attgctgacc agttatcgtg
     5341 agagtaattc atcggcacgt taaatcatat caggcgtaat accacaaccc ttaagttagc
     5401 gcttatggga gtgatccccg ccactcttcg tgtgtctggt aacctcaatg cacccttttc
     5461 tccccaatag tgtaaaacac aatgaagtag aaggagttat ttcacatcat gcaaagattc
     5521 agaccatatc tcccaatagc cttcgcgaca tgatgacttt cagaaatatc tagctgttaa
     5581 caaaattcca tacttg
//
LOCUS       DAPGRN010000207         5563 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPGRN010000207 DAPGRN010000000
VERSION     DAPGRN010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.209"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            166..2598
                     /locus_tag="RHW33_004573"
     CDS             166..2598
                     /locus_tag="RHW33_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV1647999.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKIAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDEEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2665..4134
                     /locus_tag="RHW33_004574"
     CDS             2665..4134
                     /locus_tag="RHW33_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV1648000.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4134..>5563
                     /locus_tag="RHW33_004575"
     CDS             4134..>5563
                     /locus_tag="RHW33_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDV1648001.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAVEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     GGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCRIGNIVNIKSELVSPKDYLNLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRF
                     YKGQIVYSKIRPSLSKVFLAEYNGLC"
BASE COUNT         1663 a         1316 c         1330 g         1254 t
ORIGIN      
        1 tgtttatctg tctctgatat gcccagatta attcaagcaa gatataacag agatatcaat
       61 gtgatctctg ctacgtactt tacgttgaaa caccgcccta aatcatagcc aggctattga
      121 ttttatgtac aatcagaatc gtcctgataa aagcgagaac cccccatggc ggaactgaac
      181 ctaagtaacc tgacggaagc agacatcatt accaaatgcg ttatgccagc cattctcaat
      241 gcaggctggg acaacacaac gcaaatcaga caggaggtca aactccgaga cggtaaagtc
      301 atcgtacgtg gtaaaattgc ggcacgcaga acggtaaaat ctgctgacat cgtgctgtat
      361 cacaaacctg gcattccctt agccgtgatt gaagcaaaag ccaacaaaca tgaaattggc
      421 aaagggatgc aacagggcat tgaatatgcg cgcctgctgg acgttccttt tgtttttgcc
      481 accaacgggg atggctttat cttccgcgat gccaccgcag ccgaaggtga atgccttgaa
      541 aagcaaataa cgctggatga cttcccctcc cccgctgaac tctggcaaaa attctgcctc
      601 tggaaaggat acacacaagc tcagcttccg gtaattactc aggactatta cgacgatggc
      661 agcggtaaat cgccacgtta ttaccagctt caggcaatca acaaaaccat tgaagccgtc
      721 tccaacgggc aaaaccgcgt tctgctggtc atggcgaccg gaacagggaa aacctatacc
      781 gcattccaga tcatctggcg cctgtggaaa tcaaaaaata aaaaacgcat tttgttcctt
      841 gccgatcgca acattctggt cgaccaaacc aaaaataatg atttccagcc atttggtacg
      901 gcaatgacca aagtcagcgg acgcaccatt gatcccgctt atgaaattca cctcgcgctc
      961 tatcaggcta taactggccc ggaggaagac caaaaagcgt ttaaacaagt cgcaccagat
     1021 ttcttcgatc tgatcgtgat cgacgaatgc catcgcggca gcgcatccga agacagcgcc
     1081 tggcgagaga tccttgatta ttttagttcc gccacccaaa ttggcttaac cgccacgcca
     1141 aaagagacgc atgaagtctc cagcacggat tacttcggcg atccggttta cgtctactca
     1201 ctaaaagaag ggatcgaaga cggcttcctc gccccttata aagttgtccg tgttgatatt
     1261 gatgttgatc tgcaaggctg gcgcccaacc aaagggcaaa ctgacttaaa cggcgaagtg
     1321 atcgacgatc gtatctataa ccagaaagat ttcgatcgca cgatggtaat cgacgaacgc
     1381 acagaactgg ttgccagaac cattaccgac tacctcaagc gcaccaatcc gatggataaa
     1441 accatcgtct tctgtaacga catcgatcat gcagaacgta tgcgccgcgc cctggttaat
     1501 ctcaacccgg agcaggtgaa aaagaacgac aaatacgtca tgaaaatcac cggcgatgat
     1561 gaaattggca aagctcagtt ggataacttc atcaacccaa aaaaaccgta cccggttatc
     1621 gcgaccactt cagagctgat gaccaccggt gtggatgcta aaacctgcaa actggtagta
     1681 ctggaccaga acatccagtc gatgaccaaa ttcaagcaga ttatcggtcg tggtacacgc
     1741 atcgacgaac gttacggcaa actctggttt accatcctcg actttaaaaa agccaccgaa
     1801 ctgtttgccg atgagcgttt cgatggcatt cccgaaaaag tcatggatac cacaccagag
     1861 gatatcgccg atccagaatc tgattttgaa gagaaactcg aagaaatcag cgaacatgac
     1921 gaagaacagg taacaggcgt tgatgaaccg cctgcgccac cataccaggt taccgatacc
     1981 gatgatgtcg gcccacttcc ggaagaagac gagaagaaaa tccgcaagtt tcacgtcaac
     2041 ggtgtagcgg tgggcgttat tgcgcagcgt gttcagtatt acgatgccga cggtaaactg
     2101 gttaccgaat cttttaaaga ttacacccgc aaaacactgc tcaaagaata tgcctcgctg
     2161 gatgacttta cccgcaagtg gcaggacgcc gatcgcaaag aagcgatcat tcacgagctg
     2221 gagcaacagg ggatcatctg ggaagtactg gcagaagaag tcggtaaaga tctcgacccg
     2281 ttcgacatgc tttgccacgt agtgtatggt cagccgccgt taacccgcaa agagcgcgcc
     2341 gagaacgtgc gcaagcggaa ctacttcaca aaatactctg aagcagcgca agccgtgctc
     2401 gataatttgc tggataaata cgccgatgcg ggcgtacagg agatcgaaag tattcaggtg
     2461 ctgaaactta aaccattcga cagcatgggc accttaccgg agattattaa aaccggattt
     2521 ggcgaccgta acgggtataa tcaggcgctc agcgagctgg aaaacgaaat ctaccaatta
     2581 ccgccccgct ctgcttaaca accctctttt gcgccttctg caaccatcat gttgcagaag
     2641 gccttgtttt tcatggaata caacatgtct atcagctcag taatcaaatc attacaagat
     2701 attatgcgca aagatgccgg tgtggatggc gatgcgcagc gtctcggtca gctctcctgg
     2761 ctgctgtttt tgaaaatctt cgatgcccag gaagaggcgc tggaactgga gcaggataac
     2821 tatcaatatc cgatcccaca gcgttatttg tggcgcagtt gggccgccaa cgcgcagggc
     2881 attaccggtg attccctgct ggaattcgtt aatgatgatc tgttcccggc gttgaaaaac
     2941 ctcacggcgc ctatcgataa aaacccacgc ggctacgtgg tgaagcaggc gttcagcgat
     3001 gcctataact atatgaaaaa cggtacgcta ctgcgtcagg tgatcaacaa gctgaacgaa
     3061 attgacttta ccagcgccag cgaacggcat ctgtttggtg atatttacga acagatcctt
     3121 aaagatctgc aatctgcggg caatgctggc gaattctata ctccacgcgc cgtcactcgc
     3181 tttatggtgg atcgcgttga tccgaaactc ggcgaatcca ttatggaccc ggcctgcggt
     3241 acgggtggtt ttcttgcctg cgcgttcgat catgtaaaga acaaatacgt gaagagcgtc
     3301 gccgatcatc agacgctgca acaacagatc cacggtgtcg agaaaaaaca gcttccgcac
     3361 ctgctggcga ccaccaatat gctgctgcac ggcattgaag taccagtaca aattcgtcac
     3421 gacaacaccc tgaacaaacc gctttcctcc tgggatgaac aactggatgt cattgttacc
     3481 aacccgccgt ttggtggcac ggaagaagac ggtattgaga agaactttcc ggcagagatg
     3541 caaacccgcg aaacagcgga tctgttcctg caactgattg tggaagtact ggcgaaaaac
     3601 ggtcgtgcgg cggtggtatt gccggatggc acgctatttg gcgaaggcgt taaaaccaaa
     3661 atcaaaaagc tgcttaccga agagtgcaac ctgcatacta tcgtgcgttt accgaatggg
     3721 gtgtttaacc cctataccgg cattaaaacc aacctgttgt tctttaccaa aggtcagcca
     3781 accaaagaga tttggttcta tgagcatccg tatccggcgg gtgtgaaaaa ctacagcaaa
     3841 accaagccga tgaagtttga agagtttcag gcggagatcg actggtgggg taacgaggcc
     3901 gatggttttg ccagccgcgt cgagaatgag caagcgtgga aagtcagcat tgatgacgtg
     3961 attgcgcgta acttcaatct ggatattaaa aacccacatc aggcggaaac cattagccat
     4021 gatccggacg aactgttagc gcagtatgca aaacagcaag cggagatcca gacgctgcgt
     4081 aatcaactgc gcgatattct tggcgctgcg ctgtctggca aggaggttaa ctaatgagtg
     4141 tggaaaagct gatcgttgat catatggaaa cctggacctc ggcgttgcaa acccgttcca
     4201 ccgccgggcg cggcagttcc ggaaaaattg atttatatgg cattaagaaa ttacgtgagc
     4261 tgattctgga actggctgtg cgcggtaaac tggtgccgca ggatccgaac gatgaaccgg
     4321 cgtcggagct gctgaagcgt attgcggtgg aaaaagcaga gctggtgaaa caggggaaaa
     4381 ttaaaaagca aaaaccactg ccggaaatta gcgaggaaga gaagccgttt gaattgccgg
     4441 gtggatggga gtggactcga ttaattaatc ttggtatatg ggctttaggg tctggttttc
     4501 caaatgtggt acagggaagc actgataaag aaattttgat gtgcaaagtt agcgacatga
     4561 atcttgaagg taacgagaaa ttcatttttt cgacaaaaaa tacaatttca aaagacctag
     4621 ctgatgaata taaaattaaa atctcagaac ccggcaccat cattttccct aaaattggcg
     4681 gagcaattgc aactaataaa cgaagaattt tagttcagga tacagctatt gataacaact
     4741 gtcttggaat aaaaccatgt gatgcaatat cgggggagtg gttctatcta atccttaata
     4801 ctctcgatat gtcgaagtat caatcaggaa cctcaatacc cgcgataaat caaagtgtca
     4861 taggtagtat accaattgcg cttccttctt taaaaatgca agagaagatt gtttcttatg
     4921 tcataaccct aatgtccctc tgcgaccaac tggaacagca atccctgacc agtctggacg
     4981 cacatcagca actggttgaa accctgttgg gaacgcttac agacagccaa aacgccgagg
     5041 aactggctga aaactgggcg cgtattagcg agcatttcga caccctattt accaccgaag
     5101 ccagcgtgga tgcgttaaaa cagaccattc tgcaactggc cgtaatgggc aaacttgtgc
     5161 cgcaggatcc gaatgacgaa cccgcctctg aactgctcaa acgtattgcg caggaaaaag
     5221 ctcaattggt gaaagaaggg aaaataaaaa aacaaaaacc actgccgcca attagcgatg
     5281 aggaaaaacc gtttgaactt ccggaagggt gggaatggtg tcgtattggt aatatagtta
     5341 atattaaatc tgaattagta tcaccaaaag attatttaaa tttatatcaa gtagcacctg
     5401 atattattga aaagggcact gggagagtga tatcaaagag aacagtgaaa gaatcaggag
     5461 tcaaagggcc aaatagtcgc ttttataaag gacaaattgt ttattctaaa atccgcccgt
     5521 cattatccaa agttttttta gcagagtata atggtctttg cag
//
LOCUS       DAPGRN010000208         5521 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPGRN010000208 DAPGRN010000000
VERSION     DAPGRN010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.210"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2684)
                     /gene="traG"
                     /locus_tag="RHW33_004576"
     CDS             complement(<1..2684)
                     /gene="traG"
                     /locus_tag="RHW33_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDV1648002.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPLLYAILNSAMTFYAKQNGAPV
                     LLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIRGEENIVGRQYSELQ"
     gene            complement(2681..4054)
                     /locus_tag="RHW33_004577"
     CDS             complement(2681..4054)
                     /locus_tag="RHW33_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDV1648003.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTR"
     gene            complement(4054..4467)
                     /gene="trbF"
                     /locus_tag="RHW33_004578"
     CDS             complement(4054..4467)
                     /gene="trbF"
                     /locus_tag="RHW33_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HDV1648004.1"
                     /translation="MNHRKYQRELIMRENKSNPELKIRSTERDYKYISRITGRYAGLS
                     LVFLTAGIVLWTVMDIIFDACIDSWKADPELNNSSYMWNILIYAIPYTLYALAAGFLV
                     TFFSVPNVRINIRKYRDIPAEMSYAPGEHIKGGQE"
     gene            complement(4421..4762)
                     /gene="trbJ"
                     /locus_tag="RHW33_004579"
     CDS             complement(4421..4762)
                     /gene="trbJ"
                     /locus_tag="RHW33_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDV1648005.1"
                     /translation="MPPVLWRGWIPFCRCTEEKKVRNKQVVLLIAGISGIVTGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLSLTFSVSVVFFVGAIVGWISVSEIYYSRMTGLNESS
                     EISEGTYNERK"
     gene            complement(4692..5237)
                     /gene="trbB"
                     /locus_tag="RHW33_004580"
     CDS             complement(4692..5237)
                     /gene="trbB"
                     /locus_tag="RHW33_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDV1648006.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FSYTLDGQGDTAFPEALPVPPEVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARVDTVLQMYGGKKGAK"
     gene            complement(5224..5508)
                     /gene="traQ"
                     /locus_tag="RHW33_004581"
     CDS             complement(5224..5508)
                     /gene="traQ"
                     /locus_tag="RHW33_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HDV1648007.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
BASE COUNT         1331 a         1518 c         1304 g         1368 t
ORIGIN      
        1 ttttgtagtt cagaatactg tcttcctaca atattttctt cgccacgtat gctgctctga
       61 gtatctccga tattgcctcg gtattctgtg accatattat caacctgatg ttttacatca
      121 ttacggatat tactgtcctg cgttctttgc tcaataatgg cctgatgtcc ctgatggtca
      181 gcaataatat cctgactgcc tccacccgaa ggtacgctct ccattccttt accaatatcg
      241 ccacgggatt cccgccaggc attatcaaca ccaggctgta tctgttcctg cacaaaagac
      301 caggccattg cccgacggcg ttctgcaatc tccggcgaac tggtatttgt cagaatagct
      361 tcggcatcct gcggtgcgtg tttcatcaca tactgtgcaa actgttgcga caaatcttcg
      421 ctcatctgcc cactcatgct ttcagtacgg gatgccattt cagcatattc atggctgcgg
      481 gtcatattcg tcgtgtactg gtcgtaactc tgtttcgctg aattcagggc tgcagacagt
      541 tgatccaccc gggaatcagc attattgtca gtatggctgc cggattcact gactttgcga
      601 ctggtaaagt aatcgctggc ctctttaaag tccttactgg ctttagcgtc aatatcatga
      661 cgagcatcat agctggcccg tgaaccactg ctggcctgat gcgcatcatc atcactccag
      721 tcaatgccag cccgaccacc acctctaacg cctaatcctc caccaacacc aagaatttta
      781 ggtttaaccc cccattcagc atgagcatct ccatacatcc cggcagaagc tctcgtactt
      841 ctggatgcca gttcctgagt cgcctgctca ttactgatat tatgcgcctt cgcatagctt
      901 tccactgcac tgcgcatacg gctggccatc atggagtcct gtgcactcat cgtactgtca
      961 gcaccactgg tgacagagtc actgctcccc cggttagaac caaactgact gagcgtgttc
     1021 caggcactgg caatactgct gctgaaacca tgcagggcac tttctgctct gttcgacgcc
     1081 tcccgggcca tttcctgttg tgccgctgca atctgacgcg ttgcattgat accgaccggt
     1141 aaacgggaca tcgctccgct tgcatccatc accatattac cgtcacgggt ctgtgtggcg
     1201 gttgcgccac tgccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc
     1261 cagctgaagc cgttcacgtt ttccgtctgc atgttaccgt aggagtaatt accgtcaacc
     1321 acactacctg ccgcactggc agtcgggctg atagcagagg aagcgaaatg gctgtacaca
     1381 ctggaaaatc cggccccaag ccccctgacc atcatccagg ataatggcgg gatcatcatg
     1441 gaaagatacc cggcagtgga agccagatcg gagtatttca gttgtatctg tgaaatttca
     1501 gagagcaaca ccggcgcacc attctgtttc gcataaaatg tcatggcact gttcagaatg
     1561 gcgtacaaga gtggccatga ctgcagccac atcagggcaa acacataacc tttcaggaca
     1621 gacagcgtca gtttgttgaa tacagccgcc agtaccagga gcgggaaaat accgatggcg
     1681 atccccgtca ggatggtctg cgtcatgggc agtgtccgca tcgccacatg accgatggag
     1741 acatgtgcca gacgttgttt ctccatcgat gacgtggtgg ccagattcac caggctggcc
     1801 gaatcaccat tacgtgcggc ataactggtg atcccctcct tcagcgcatt gatggtgaca
     1861 ttctggcgga tgatctggct ggctgattta cttgagccat agaagtaact gtaactgtcg
     1921 ccaatcagcg tactgaacag caggttagga tcagggcgtc caccaaatag ctgctgggca
     1981 taataatgcc aggtttttcc gccactctgt gtgtccagat tcagcttgtc tttcagcgaa
     2041 accgacgcgt ccttacaggt tacgaaatta ttattgctgt cataaacgcc ccgcagcggg
     2101 ctggggcggg aaaaaatcag cgtgtaggga tcagcggagg ccatcagctc ctccagcgtg
     2161 tatttgtggt tcaggtaaat atcccccatc acacagttct gcacataatc ctggaaaaga
     2221 ttggcgattt ccggattgcg ggacaaaaag tcggtgcttt ttgataccag ttctgcgcca
     2281 aacagcatcc ccgttttgct gtaggtgaca ctgtccggtt gcgtgaagat catctcgtaa
     2341 ctggccacca ttgcatgccc gatacgggtc gtcagtgaaa gtggcatcgc cagtccgacc
     2401 ggcacattat ccacccggtg aactttgacc aggtcactgt tatcaataat ctgcaccgat
     2461 gtgcggacat taaccagcag gctgataagc acaaatacgg ccacccagcc cagcaaatcc
     2521 atcacgttgt gccgctgtac ccacattacg gccaccgcga gaacagacaa tgtgagcgca
     2581 attttttcaa tggaatccca cgtcctggtt cccataaagg cggcaatggc gttcaggtta
     2641 ttccgcaacc actcaccacc ggcaatcaca taaacttcat tcaccgcgtg ctccctccga
     2701 agtgatagtt gttctggtaa cgactgagca tgcgggcgga aagctgctga cgcatgtagc
     2761 tcatctgacg atcgacaacc agcaacgcat cctgctgtac ctgcacctgt gactgaaacg
     2821 ccgcaatctg ccgggtggca tcattcatgt tgtcgttaat atgtccgata accgcttcgt
     2881 cataatttcc cgtggccacc atcgcccggg cctgctgtat cagctcctga atgtactgca
     2941 gcaggatgtc gtaaccgata tagtccgtca gctggtaaat catgctgttg gaaactccga
     3001 gcatctgcgg gtcaaccagg tatttgaaga cggggatggt ggtactggaa ataaagcctt
     3061 tttccttgtc atccagaggc gtgtcactga ccgccttgtt ctgaatgctg gccagaagtt
     3121 tagtaatctg agacttcagt gcattatccc ggctgatagt caccggtgtg tctgccacca
     3181 ctttcaggca tttatcagaa tcgttgcagt gataaatttt tgctgtgccg ccttccatca
     3241 tggcccggat aattgagcgg tcagtggttc tggctgacag cggtgtgatt tcgccgttgg
     3301 gaccaaacac cagtgagccg gtcagcgtca tcacaaattc tttcagctct ttgttgccgt
     3361 caaacattct gtttttggaa agcgcattcc acatgatgtt gatgtttttg gtcacccgct
     3421 ccttatcctt gtcgctggct ttatccctga cactgtcaga tttcccgcca acggtacatc
     3481 cctgccggga agccgcccag tcagcaaaaa tattgctctc accggcaatg tcctgacaga
     3541 ctttctgctg ggacacctgc gtccggggga aaagcccgcc gataatcccc tgtgcagcct
     3601 ggcaggaact gaggttcata ctgttaatgt cgcttgccat tttctgcacg aagtcttttg
     3661 cggtttttat ttccggcacc gttgtctgca gggcaaggtc aaaaaagtaa ccggcagcat
     3721 tgctcataat ctgcttaaca aaacgctgca actgctcacc attaataaaa ctgaacgagc
     3781 cgagataggc gtcgatgccg ccacatccag cattgatatc cggcagcgtc atggaaatta
     3841 gctgaacatt tttgacctga gtacgggcat acagggagcc accgtaagca tagccggcgg
     3901 cctgcccctg ccagacgccc ggctgtgtgg tattggaagc aaatcccagc ttattaaaga
     3961 actgattcat gtcgctgttc acatccgctg acgctgcagg cgtcaccgtc agtaaggcgg
     4021 cacaaagaac cagaagaggt ttaatgcgtg gcatcattct tgccctccct taatgtgttc
     4081 tcccggtgca tacgacattt cagcaggaat atctcgatat ttccgtatat taattcgcac
     4141 attggggaca gaaaaaaatg taacaagaaa cccggctgca agcgcatata acgtatacgg
     4201 aatggcatat atcaggatat tccacatata actcgaatta tttaattccg ggtccgcttt
     4261 ccacgaatca atacaggcat caaaaataat atccataact gtccataaaa caattccggc
     4321 agtcaggaat accagagaca acccagcata cctgccagta attctggaaa tgtatttata
     4381 atctcgttct gtacttctga ttttgagctc aggattactt ttattttctc tcattataag
     4441 ttccctctga tatttccgat gattcattca gaccggtcat gcggctgtaa tatatttcag
     4501 acaccgaaat ccatcccaca attgccccca caaaaaacac aacagaaaca gaaaaggtaa
     4561 gactcagcga aacgacaatt tctacagggc tggcgggata aaaaagcccc tgacggataa
     4621 aggggatatt caggctgacg attattcccg tcacgatgcc ggatattccg gcaatgagta
     4681 acaccacctg cttatttcgc accttttttt cctccgtaca tctgcaaaac ggtatccacc
     4741 cgcgccataa aaccggcggc atccgttgcc ccctgtaaaa gcggtaatgc ctcaagcgtg
     4801 ttgacgttga ccaggaaggt ggtcggtgtg gccaccggga tattcgggaa gaaggtctgc
     4861 atcacctccg gtggcaccgg taatgcttca ggaaaggccg tatccccctg accatccagg
     4921 gtgtaggaaa aaacggaaaa gccgtactgc tgtgccagct gtttcagtac cgggtcaaac
     4981 tggtgacagt aagggcaatg cccctgcata aacagcacca ccttccagtc ggccaggtta
     5041 acctgcctgc cattactgag gcggaaccag cggggagccg gctttgctgt cctggctgac
     5101 gtgcctgctg ccggttgtgc aggctgcgta gccataccct gcggattcca gagtcgctct
     5161 atttcatccc gggtggaggc ctgcacagca gcggcgctca gcaacagggt gaacagcagt
     5221 gatttagtga gagacatgtc cgccctcctg ctgcccttcc atcatggcct gttgcctggc
     5281 ttccagtgcc tcacgctctt cccggctgac acgggcaatc catgcatcca gcacctggta
     5341 tgcgccgaac agtacgagaa tggccgcaat cagctccgcc agaaaaaaag ccatccaggg
     5401 tttgctgtaa acaagacggg cgacgatatg aaaccagaca cccagggaaa atacccacat
     5461 tcccgtaata tcaagccggg gtaaagagaa tctgcgttta cttatcatgt taaactccgg
     5521 t
//
LOCUS       DAPGRN010000209         5468 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPGRN010000209 DAPGRN010000000
VERSION     DAPGRN010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.211"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(219..287)
                     /locus_tag="RHW33_004582"
                     /pseudo
     CDS             complement(219..287)
                     /locus_tag="RHW33_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            656..1183
                     /locus_tag="RHW33_004583"
     CDS             656..1183
                     /locus_tag="RHW33_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1648008.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWDDNGITRYITEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            1241..1474
                     /locus_tag="RHW33_004584"
     CDS             1241..1474
                     /locus_tag="RHW33_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HDV1648009.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1533..3491
                     /locus_tag="RHW33_004585"
     CDS             1533..3491
                     /locus_tag="RHW33_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDV1648010.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETALSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAGSIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRALAQEGKTPAQTG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALESDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVKDNTKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPVQISLECKHYSLTSDAPSGKEGAAFLVLMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            3546..3980
                     /gene="psiB"
                     /locus_tag="RHW33_004586"
     CDS             3546..3980
                     /gene="psiB"
                     /locus_tag="RHW33_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV1648011.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNCGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            3977..4696
                     /locus_tag="RHW33_004587"
     CDS             3977..4696
                     /locus_tag="RHW33_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV1648012.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            4693..5289
                     /locus_tag="RHW33_004588"
     CDS             4693..5289
                     /locus_tag="RHW33_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648013.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLTWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSSVVREAQDWLCDRKLVDITDILR"
BASE COUNT         1183 a         1472 c         1695 g         1118 t
ORIGIN      
        1 tcagaagata gtgtatggct gtatgccagg aacaggtgcg atattccgtg acactgtcac
       61 tgtttctggc tgtcttttat tgcgcccact tcggctcccg gctgcctggc ggcatccggt
      121 cgtcaggccc gactacgaca ggcagctgct tgcgcacccg ccagtctccg ccgaacccgc
      181 tggcgcggtt tcgggctgtc gctacggtcc gatgcgtgaa aaaccgggct tgcagcgacc
      241 gttccggtcg cgccgcctct gtcgcgccgt gacctgcgct ccctgcacac ctgacggtgt
      301 gtgaggaaac tgcgcctgat ggcgcagcag tcttccctcc tgccgccacc tctgcggcct
      361 gtttcccgct gtggactttc cggggggcag acgtaacccc ctcccgttcc atctgtccag
      421 ggtgagatgc accggccaca aaatttttag ccatgaaccc ggcaaaaaat tttgcgtccg
      481 cccccggaca gctgtcccgt gaggcggtaa acgggccccc atcggaaagg tccactgcgg
      541 ggagaaaccc ctgtgaagtg gacggcagga ggagctttcg ctcctctgaa ggggcacatg
      601 aacgtcccgt ctccggtatc cggtttatct cacatctttc agtcaggagg ttcttatgag
      661 tgcacgtggt atcaacaagg tcatcctcgt cgggcgtctg ggcaatgatc cggaggtccg
      721 ttacatcccc aacggtggcg cagtggcaaa cctgcaggtg gccacgtcag aaagctggcg
      781 cgacaaacag acgggggaga tgcgggagca gacggaatgg caccgtgttg tgctgttcgg
      841 caagctcgcg gaagtggcag gtgaatatct gcgcaagggc gcgcaggtct acatcgaggg
      901 gcaactccgt acccgtagct gggacgacaa cggcatcacc cgctacatca ctgaaattct
      961 tgttaagacc acgggcacca tgcagatgct ggggagtgca ccacagcaga acgctcaggc
     1021 gcaaccgaag cctcagcaga atgggcagcc acagagtgct gacgcgacga aaaaaggtgg
     1081 cgcgaaaacg aaaggccgtg gacgtaaggc cgcgcagccc gagcctcagc cgcaaacgcc
     1141 ggagggggag gattacgggt tttcagacga catcccgttc tgatcgggct gactgtgaca
     1201 accgccccgc cctgtgcggg gcatcaccgg agatatgagg atgagcgaat atttcagaat
     1261 acttcaggga ctgccggacg gctcctttac ccgcgaacag gcggaagccg ttgccgcaca
     1321 gtaccggaac gtctttatcg aggatgacca cggagaacat tttcgcctgg ttgtccgtaa
     1381 taacggaaca atggtctggc gtacctggaa ttttgaggac ggtgccgggt actggatgaa
     1441 ccatgccatc cgtgatttcg ggattcttaa gtaagagagg tgccggacgc gcgaacgtcc
     1501 ggcaggacat aagcaattat aaggggatga ttatgcctgt aacgaagtgt gaaccagaaa
     1561 ccacccgcaa agcaagccgt aaatctgcaa aaacgcagga aactgccctg tctgccctgc
     1621 tggcgcagac ggaggaagtg agcgtgccgc tggcctcact gattaaatca ccgctgaatg
     1681 tgcgcacggt gccgtattct gtggagtccg tcagcgaact ggctgggtcc attaagggcg
     1741 ttggcctgtt gcagaatctg gtcgtgcata ccctgcctgg tgaccgttac ggtgtcgccg
     1801 caggtggtcg ccgactggct gcactcaaca tgctggcaga gcgcaacatc cttccggctg
     1861 actggcctgt ccgtgtgaag gtcatcccgc aggaactggc gactgccgca tcgatgaccg
     1921 agaacggtca tcgtcgggat atgcaccctg ccgaacagat tgccggattc cgcgcattgg
     1981 cgcaggaagg caaaacacct gcacagactg gcgacctgct gggctattca ccccgccacg
     2041 ttcagcgaat gctgaaactg gcagaccttg cgcctgtcat cctcgatgcg ctggcagagg
     2101 accgcatcac caccgagcac tgtcaggcgc tggcgctgga gagcgacacc gcgcgtcagg
     2161 tgcaggtgtt tgaagcggcc tgccagtcag gatggggcgg taaaccggat gtgcgggtta
     2221 tccgcaacct gattacggaa agtgaagtgg cagtgaagga caacactaaa ttccgctttg
     2281 tgggggctga tgccttctcg ccagacgaat tgcgcaccga tttgttcagc gatgacgggg
     2341 acggttatgt cgaccgcgtg gcgctcgatg ccgccctgct ggaaaaactc caggctgttg
     2401 cagagttcct tcgggaagcc gaaggctggg aatggtgcgc cggacgtatg gagcctgtcg
     2461 gtgagtgccg tgaggatgcc ggaacatacc gctgtctgcc ggagccggaa gcggtgctga
     2521 cggaggcgga agacgaacgc ctgaacgaac tgatgacgcg ttacgacgcg ctggaaaacc
     2581 agtgtgagga atccgacctg ctggaagcag aaatgaagct gatgcgctgc atggcgaagg
     2641 tcagagcgtg gacgccggag atgcgtgccg gaagcggtgt ggtggtgtcc tggcgttacg
     2701 gtaatgtgtg tgtccagcgt ggtgtgcagt tgcgcagtga ggatgatgcg gctgacgacg
     2761 ctgaccgcac ggaacaggtg caggaaaaag cgtcagtgga ggaaatcagc ctgccgttgc
     2821 tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc ggcactgatg cagcagccgg
     2881 acaaatctct ggcactgttg gcatggacgc tctgcctgaa tgtgtttggc agcggagcgt
     2941 acagtaaacc agtacaaatc agtctggaat gtaaacatta ttcgctgacc agcgatgcgc
     3001 cgtcagggaa ggaaggtgcc gcattcctgg tgctgatggc agaaaaagcc cgtcttgcgg
     3061 ccctgttacc ggagggatgg tcacgggaca tgacgacgtt cctgtcactc agtcaggagg
     3121 tgctgttatc cctgctcagt ttctgcaccg catgtagcct taacggtgtc cagacccgcg
     3181 agtgtggtca cacgtcacgc agtccgctgg attcgctgga gagcgccatc ggttttcaca
     3241 tgcgcgactg gtggcagccg acgaaagcaa acttcttcgg acacctgaaa aagccgcaga
     3301 ttatcgcagc cctgaatgat gccggactgt ccggtgccgc acgggacgcg gagaagatga
     3361 agaaaggtga tgcggctgaa catgcagagc accatatgaa agacaaccgc tgggttccag
     3421 gctggatgtg tgcaccacat ccacagacgg ataccactga acgcaccgat aacctggctg
     3481 atgccgcctg atgaacaacc acaccgcccc gccggagacg gggcggcagc aagggagata
     3541 ccgtgatgaa aactgaactg accctgaatg ccctgcagtc catgaacgca caggaatatg
     3601 aagatatccg tgctgcggga agcgatatgc gccgtaatct cactcacgag gtgatgcgtg
     3661 aagtggacgc accggctaac tggatgatga atggcgagta tggcagtgag ttcgggggct
     3721 ttttccccgt ccaggttcgt ttcacgccag cccacgaacg tttccacctg gcattatgtt
     3781 cgccgggaga cgtctctcag ctctggatgc tggttctggt gaattgtggt ggacagcctt
     3841 tcgccgtcgt tcaggtgcaa catatcttca cgcctgtcgc tatcagtcac acgctggcgc
     3901 ttgccgcgac acttgatgcg caggggtaca gtgttaacga catcatccat atcctgatgg
     3961 cagaaggagg tcaggcatga gcgcacgttc acgggcactg atccccctca gcgcagagca
     4021 acaggccgcc atgcaggctg tggctgtcac cgaacaacgt cgtcgtcagg gacgcacact
     4081 ttcagcatgg ccttatgcca gcgctttctt tcgctgcctg aatggcagtc gccggatttc
     4141 gctgaccgat ctccgctttt ttgctcctgc gctgacgaag gaggaatttc atggcaaccg
     4201 tctcctgtgg ctggctgccg tggataaact gattgaaagt tttggtgaag tctgtgttct
     4261 tcccctgcca tccgatgcgg ggcatcgtct gttcccgtcc gttccttttc gtgaaggtga
     4321 gcggcgtcgt cagaaaacca cgctgacaga gcagaaatac agccgccagc gggaacgtga
     4381 ggcagaacga cgggaactgg aataccagac atgttttgct caggcgcaga ttgaccttgc
     4441 gtttcatact cccgccacgg tcggaagctg gttgtcccgc tggtctggtg ttgttgagga
     4501 gcatgatctg gaaacgattt tctgggggtg gtgcgggcgt tttccatcac tgtcatcatt
     4561 tgaccggttt ttctggcagg aggaaccact ctggcggctg atttttgaag ccggtgaggc
     4621 cggtcgtggt gcaccggtac aggtacgtgc acttgagcag tggatgatcc cgaacaagct
     4681 ggagaacgca atatgatgaa atcagacgaa caatatcagg ttcccgtgtg gatgcgacct
     4741 ctgttgccgt tgctctgcaa taccggtggt aatgatccgg aggaactgct gaatgataca
     4801 gaaaccaccg ccagtgcgaa tattgtccgt tatgtactga tagttgcagt gcggtcgcag
     4861 attgatctgc tgcagctact gtacaggaaa ggactgttgc gcacagagat accaggtggc
     4921 ttttcaccgg aagaagcgca ggaactcctg gataatctgg tgcgcagcca tatcagcaag
     4981 gcgttgtcgg gtgagcgaat ggcagcccgt gacagaaatg ccgatctgac ctggattcgc
     5041 cagcaactgg tcgatgccgc ctggtttgtc cgtgccacac tggaagcgca tgggatgggc
     5101 gtcggaaatg agagtccctc tgctccgccg gagacaatgc cggacataca gacacgggaa
     5161 ctggttatgt tgatcaagcg actggcatca tcgctgaaag cggtgaaacc cgacagtagc
     5221 gtggtgcgtg aagcgcagga ctggctgtgc gacagaaaac ttgtggatat cacagatatt
     5281 ctccggtgaa ccagacatgt atattttacg cagggacacg ccgatcatga tcgtttcata
     5341 tcaggggtgg tctggaggtc atgcggcgtg tcctctgcac tcgccggaat aaggaagtcg
     5401 ccggcggctc cgcttttacc cggccatgcg gggcatggcc ttgtgggttt tcagctctgt
     5461 ggcctcag
//
LOCUS       DAPGRN010000210         5380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPGRN010000210 DAPGRN010000000
VERSION     DAPGRN010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.214"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..283)
                     /locus_tag="RHW33_004589"
     CDS             complement(<1..283)
                     /locus_tag="RHW33_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648014.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCGVVEASGASLEKGRNDQLLTALHALLLSRKNPFGDIKSDGTVKTAL"
     gene            complement(288..872)
                     /locus_tag="RHW33_004590"
     CDS             complement(288..872)
                     /locus_tag="RHW33_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDV1648015.1"
                     /translation="MDVTNDDYIHLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINENFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(863..1921)
                     /locus_tag="RHW33_004591"
     CDS             complement(863..1921)
                     /locus_tag="RHW33_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDV1648016.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPIACSSAGAVGNADDGTSLILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIEPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(1908..2333)
                     /locus_tag="RHW33_004592"
     CDS             complement(1908..2333)
                     /locus_tag="RHW33_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HDV1648017.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMIDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(2333..2881)
                     /locus_tag="RHW33_004593"
     CDS             complement(2333..2881)
                     /locus_tag="RHW33_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HDV1648018.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNSPKARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(2881..3960)
                     /locus_tag="RHW33_004594"
     CDS             complement(2881..3960)
                     /locus_tag="RHW33_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HDV1648019.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNNGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFESRQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(3957..5285)
                     /locus_tag="RHW33_004595"
     CDS             complement(3957..5285)
                     /locus_tag="RHW33_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HDV1648020.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKVTFRPSITAYVVGDDCFDQRDRLIEALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
BASE COUNT         1127 a         1666 c         1391 g         1196 t
ORIGIN      
        1 cgagagccgt tttcaccgtg ccatccgatt tgatatcacc aaacggattc ttgcggctta
       61 acagcagcgc atgaagcgcg gtaagcaact ggtcgtttcg ccccttctcc aggctggcac
      121 cggatgcctc caccacgcca caaagctctt cctgcaacat gtcaaagtag tcatcatcca
      181 gatcggtagc aggcgtgcca gtctgggggt taccacgggt aaaaccgttc ttacccgcgc
      241 cgaacttatc cttctgcgcg gttttagtgt ctatgcgatg catgggatta ctccggatat
      301 ttaaaaatta cgtaggtatg cgacgggcag agtttgttaa gcacacactc gacaacggtg
      361 tcaccccaga tacgcagtgc ggaatcacag ggatcgccac atgtcatcca ggtggtgttg
      421 gtggcggctg gcatgttgac ctgccagtaa taccgccatt ccggcgcatt caccgcgtca
      481 gtacaggccg atgagcaggt gaacgtgctt ttgtcgtatc gcgtgatggt agcgtctggt
      541 ctgcccaggg cagcaagctg tgcaagataa aaattctcgt tgatgccgcc cgccaggtta
      601 accttcgcat ccagccgttg ctgacgctgg cgaagggtct gtgtccctgc gggaatacat
      661 tcatccggca gaccacacag acgctcccag cggtttatca gttcagtggt ggtgcgcgga
      721 tccagctccc gcatcagggc atccgcacgc tgatgaacac gggttaatga aggtgccgca
      781 ccggcaatcg ccggatcgct ggctgaccac gccggaccgg ggggcaacag tgccgacaac
      841 agatggatgt aatcatcgtt tgtcacgtcc atgaaatcgt ccccagtacc gccagttcat
      901 tttttgcaat ggagatattg tccgccggtg caagcaactg atggctgtat tccccgttcg
      961 caccggaaat cgcctcactg atacgcgaca ccttcagttc tccctgcgga taaccatcac
     1021 gcagcaggaa cgaacgcaac tcggcggtga tggcagcccg tatttccggt gtgtccggcg
     1081 tcacgcggat atgaaaatcc actttatgcg ccaccggcct gaatacatac aaatcagagc
     1141 ctgctaccgg ggccagtggc tcaatgtgtt gtcttgccgc cgtttccgtt gattcttccg
     1201 gaatgggatt aatcaggtca ctgctggcaa tcatcacacc gacagtcccc gttcccatcc
     1261 agtggcggta tgtccatgca cgggtaatgc ctggcacttc tttagcccag acaacatagt
     1321 ccccgtcagc cccgccctga ggcgtccagt aataccgctc aatgacgcgg gcgcgccacg
     1381 tttccagctc ttcagtatca aatccacctg tcagagtgtc tgccacgccg gaagacggca
     1441 gaccattcac cggcgtgacc aggattaatg acgtaccgtc gtcagcgtta ccgaccgctc
     1501 ctgcacttga gcaggcgatc ggcacgcgca ggacaccacc ggagctggtt gcatcggcag
     1561 ttgccgtgta ctgaaccagg tcatcgcgct gaataacact cccggcggtc accttcaggc
     1621 catcgctgac accttcccag cgcatatacc cgctggcagc cgtggccccc ttgcgcggac
     1681 accgtttcat cgcagcatgt cgcgccagcc aggactcatc gcacaggtca ggcagcatgt
     1741 tcattgccag ataatcgatg taaccgtaaa ccgtatgcag cgccgccgca tacacctttg
     1801 cccgcacgtc ttcatccatg cgccggagcg tgtcgctgac gtccagcctg gcgaataaat
     1861 cgttacggag catactgata ttttctgcca gcgtcgggcg ctgaaattca ctgtccgcca
     1921 tgcgttatcg cactccacag atcatcaaaa gaaatcatta ccggtccgtc acgacgccag
     1981 agagtgatac tgttacccag ttcattaatc ccggtgcggc ggatatccag atcaatacgg
     2041 gacaccacgc cgtcatcaat catccattgc aggcattcgc ggatataccc ccttaccgtc
     2101 tgcaccagct gattggtcag tttgctgcgc tgaagcagcc acagtcggga gccgtaacgg
     2161 tcattctgta ccgcaggcca ggtatccccc caccatccca tcgggacgtc ggcgttgtca
     2221 tcaggctccg cccgccgcca ggtaaacagg gaaatcacca cggcgcgggt cagcggatcc
     2281 agcggtgcgc tggcgcaggt gcgtttaccg ttcaccgtca gccacagttc catcatgcct
     2341 ccatcgcttt atccggtttg tcggtgttac tgccctgacc gttctctctg tgacgatgcc
     2401 cgttataggc aagccgcatc gctgacatgg tggtgccgcc ggagtcgcac aggtctttca
     2461 cctgtcctgt cacttccagg tccatttcaa aacgtgcttt aggtgaattg cgaaacgtga
     2521 tcgttttacc tgcaccgtcc accacgatcc cctcccgggt cagcgtcacg gactgcccct
     2581 gatcgtcata gacagccacc tcacccgtct gcagcccttt caggcggtag cgccggtccg
     2641 acaccgtaac aaccaccgca tgagaacggt cgccatccgg aaacaacacc accgcttccg
     2701 caccgctgtt tgcccttgcg gtaaaaccgt agggttcaag atgttcaacc ccggctttgg
     2761 gttcaccggc aatcagggac acatccacgg tctgacattt cgtggcggca ctgatgcttt
     2821 tcaccactgc ccgcccaatc aggccgagaa gttgtcgctg catggcttca atcgtcctca
     2881 tcagaacggg tcctcctgta ctctggcttt tttctttttc cgcgcgccgg gggcttcggg
     2941 ttcaggcaga taagcatcag gcgggccgac acggatttcc gtcagggtgc cgttctggtc
     3001 ctgagtaaac gtgacttccg aaacaagcag ttcggtattg tcgaaaccac agaccggatc
     3061 aaagacaatc acccgctggt tgggctgcca cagcgtaccg ttaccctgtc gccagccctg
     3121 caccacatag gtggtttcat ccgtccgcgc cgcccgttgt cgggattcaa agtccgcacg
     3181 ggcaatacag cctgcccccg tagcctgccc tgtctgcctg atatacatcg gacggtaacg
     3241 ggcaataaat gcgtcctctg tgcgggcccg cagcgcggtg gtggtggcct caccgaaatc
     3301 atcgtcgttt ccggcacgct gccccgccac ctggtaaaca gaaaaccgct cccggatact
     3361 cttctccgta tcgcaggaaa ggatgttttc cccgagtacc agcgcagtat gtgcccgcgt
     3421 tgagccaata ccgccaatca ccagcctgcc gtgcgggtcg tcgtaagcca gtgcctgctg
     3481 ctgaccgagt attttgttga ttacctcaat caccgtttca ccgtgatcag gctggacgtc
     3541 aggaataaca cccgacggcg caccgttgtt caccacctca atgccgaaag gcgcagcaag
     3601 cgcctgcgca atctgtacca gcgatcgtcc attaaactgt gtcggttcgg ctgcacagtc
     3661 aatcaggtca gcggtcagac tgcgtccggc aataccggtg ctgaccgaac gggcatcgta
     3721 acgaacgggc gtcgcctcca cccagccggt gatcaccagc tcatcaccaa tcagcacctc
     3781 cacttttgaa ccgtttttaa tgcgcggctg aagcgtggta ataccctcat ctcccggcca
     3841 ctggcgggtg atctccacac tgaaatcccg cgccagccgt tcaataccgg caccgatgcg
     3901 caccgatgtc cagccattcc actcccggcc atttacccgt agcgtgacgt tatcgttcat
     3961 tgcactggca ccttcagagg gatcaccggc acaaagccgg gatgcgtaat ggcattacgc
     4021 cggataatgt ccgcgtcacg cgccgcgtta tcaaaccagg tcgctgccag caccagcgcg
     4081 ggtaaaacct catccggtgt gcgctgaatg atccgtgcag actgttcaag gcgcgtgttg
     4141 atatccgcat tcagatctgc tttcacccgg cgcagcgcca gaaacagcgc atcactggtt
     4201 gtacgggaca actccttatc aattgccgta ttcagtgtgt cgcgaatgtc ggtcagttct
     4261 tcccacgttg gcaggtcaac cgtgtttttc accgccggtg cattgttcag tgccggatgc
     4321 gtgacagaag gccagccggt gctctgcgcg ggtgttgttg actgccccac tgtggcattc
     4381 tgcatcaccg cggaagttgt tggcgcaggc aatctggtga cggcatacgc cgcttcgctg
     4441 attgcggtcg tacgaagggt gctggcaacc acattacgct gctgcgtcgc cgtggcggtg
     4501 gttttactgt ccgttttcca gacgccgcgc ggttgcagat cgctgccgag gctgacaccg
     4561 gaaagcgttt tgatcatggt gaccaggtcg ctggcgttac cataaaggcg tttcccggta
     4621 cgccacattt tctgcacctg ctcaacgaaa tttttgcctg acgatggcgg cggcagaagt
     4681 accgagatat ccccctgcaa cagcctggcg gcatccgata cggcagaatc caccactttc
     4741 atcgcatcag aaacataccc cagcattatg ctggcattac cgataacgtc gttctgcacg
     4801 aaatccgcca caccatcgat actgaaaccg ctgaagctgt cactgatgca gtcatccagt
     4861 gcagaacagg atgacatcag cgtctgcgcc gtcgccgcac ctgatgtggg gtaagagagt
     4921 tctcctgctt cgacaaactt caggtcaaag cggacaatac gcccttcact tttcgatgtg
     4981 ctgacccgaa cttccccgtc aacacagact ttcagctcac catatgtcgg gtggacaagc
     5041 gtgccgggac cgggtttatt cagcgcttca atcaggcgat cgcgctggtc aaagcagtca
     5101 tctcccacca cataagctgt gatggacggg cggaaagtga cttttcccag atcttcggta
     5161 tagggcttgt cgcggttcgg atattcgtgt gtttccacac ggcgaccggt tcccgcactt
     5221 tcttcttcaa ccttaaacgg tacgccgcga aatgacgcat cctgaagcct gtctttccac
     5281 gtcatataaa ctccggatat aaaaaacccg ccaaatctgc tttgtcagtt atttacatcg
     5341 cagaagatgt ggcgggaacc taatattttt aattactatc
//
LOCUS       DAPGRN010000211         5071 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPGRN010000211 DAPGRN010000000
VERSION     DAPGRN010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.215"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1549
                     /locus_tag="RHW33_004596"
     CDS             <1..1549
                     /locus_tag="RHW33_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDV1648021.1"
                     /translation="CDKKNIHSKFREYAPTLMGALGILGTFIGIIIGLLNFNTESIDT
                     SIPVLLGGLKTAFITSIVGMFFAILFNGMDAFFFANKRSALAENNPESVTPEHIYHEL
                     KEQNQTLTKLVSGINGDSEGSLIAQIKLLRTEISDSSQAQLANHTHFSNKLWEQLEQF
                     ADLMAKGATEQIIDALRQVIIDFNQNLTEQFGENFKALDASVKKLVEWQGNYKTQIEQ
                     MSEQYQQSVESLVETKTAVAGIWEECKEIPLAMSELREVLQVNQHQISELSRHLETFV
                     AIRDKATTVLPEIQNKMAEVGELLKSGAANVSASLEQTSQQILLNADSMRVALDEGTE
                     GFRQSVTQTQQAFASMAHDVSNSSETLTSTLGETITEMKQSGEEFLKSLESHSKELHR
                     NMEQNTTNVIDMFSKTGEKINHQLSSNADNMFDSIQTSFDKASAGLTSQVRESIEKFA
                     LSINEQLHAFEQATEREMNREMQSLGNALLSISKGFVGNYEKLIKDYQIVMGQLQTLI
                     SANKHRG"
     gene            1556..2263
                     /locus_tag="RHW33_004597"
     CDS             1556..2263
                     /locus_tag="RHW33_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV1648022.1"
                     /translation="MDKIIGKQLPKKDQDNEHWVSMSDLMAGLMMVFMFISIAYMHYV
                     RIEKEKIKEVAVAYENAQLQLYNALDIEFAKDLQDWDAEIDKQTLEVRFKSPDVLFGL
                     GSTELKPKFKLILDDFFPRYLKVLDNYQEHITEVRIEGHTSTDWTGTTNPDIAYFNNM
                     ALSQGRTRAVLQYVYDIKNIATHQQWVKSKFAAVGYSSAHPILDKTGKEDPNRSRRVT
                     FKVVTNAELQIRKIIQE"
     gene            2267..3370
                     /locus_tag="RHW33_004598"
     CDS             2267..3370
                     /locus_tag="RHW33_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1648023.1"
                     /translation="MKLSIDLSELIQLGKKMLPEGVDFFLDESPIDFDPIDIELSTGK
                     EVSIEDLDPGSGLISYHGRQVLLYIRDHSGRYDAAIVDGEKGKRFHIAWCRTLDEMRH
                     KNRFERYHATNRIDGLFEIDDGSGRSQDVDLRVCMNCLERLNYKGSIDKQRKREIFKS
                     FSLNEFFSDYSTCFRHMPKGIYDKTNSGYVENWKEISKEIREKANYVCNDCGVNLSTA
                     KNLCHVHHKNGIKYDNHHENLLVLCKDCHRKQPLHEGIFVTQAEMAIIQRLRSQQGLL
                     KAESWNEIYDLTDPSVHGDINMMQHKGFQPPVPGLDLQNSEHEIIATVEAAWPGLKIA
                     VNLTPAEVEGWRIYTVGELVKEIQTGAFTPATL"
     gene            3594..3836
                     /locus_tag="RHW33_004599"
                     /pseudo
     CDS             3594..3836
                     /locus_tag="RHW33_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid mutarotase"
     gene            3910..4782
                     /locus_tag="RHW33_004600"
     CDS             3910..4782
                     /locus_tag="RHW33_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV1648024.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EGFVEWHREEVFRG"
     gene            4827..5006
                     /locus_tag="RHW33_004601"
     CDS             4827..5006
                     /locus_tag="RHW33_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648025.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISSAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         1620 a          969 c         1077 g         1405 t
ORIGIN      
        1 gtgtgacaaa aaaaatatac atagtaagtt tagagaatat gctccaacct taatgggggc
       61 attaggtatt ctgggtactt tcattggtat tattattggt ttactcaatt ttaataccga
      121 aagtattgat accagcatcc ccgtattatt aggtggccta aaaacagcat tcattacaag
      181 cattgtaggt atgttttttg ccattttatt taatggaatg gatgctttct tttttgccaa
      241 taaacgaagt gcgttagcag aaaataaccc tgaatctgtt acacctgaac atatctatca
      301 tgaattaaaa gagcagaacc agactctgac taaattagtc tcgggtatta acggtgatag
      361 tgaaggttct cttattgctc aaataaaatt actacgtact gagattagcg attcctcgca
      421 ggcacaatta gctaatcaca ctcatttcag taataaactt tgggaacaac ttgaacaatt
      481 tgcagatcta atggcaaaag gtgctacaga acaaattatt gatgctttgc gacaagtcat
      541 tattgatttt aatcaaaatt taactgaaca gtttggtgaa aactttaaag ctcttgatgc
      601 ctctgtaaaa aaacttgttg agtggcaggg aaattataaa acgcaaattg agcagatgtc
      661 agaacaatat caacaaagtg tcgagtccct ggttgaaaca aaaactgcgg ttgcagggat
      721 ttgggaagaa tgtaaagaaa ttcctctggc tatgtctgaa ctgcgtgaag tgcttcaggt
      781 gaaccaacat caaatcagcg aactctcccg ccatttagaa acctttgtcg ccatccgcga
      841 taaagctaca accgtattac ctgaaataca gaacaaaatg gctgaagtgg gtgaactgct
      901 gaaatccgga gctgcaaatg ttagtgcatc tcttgagcaa accagccagc aaatacttct
      961 taatgcagat tcaatgcgcg ttgccctgga tgaaggtacc gaaggattca gacaatcggt
     1021 tacccaaaca caacaagcat ttgcctcgat ggcacatgat gtcagcaatt cctccgaaac
     1081 cttaaccagc acgttaggtg aaacaattac tgaaatgaaa caaagtggtg aagaattcct
     1141 gaaatcacta gagtcgcact cgaaagaatt gcatagaaat atggaacaaa atacgacgaa
     1201 tgtgattgat atgttcagta agactggtga aaagattaac catcaactat ccagtaatgc
     1261 cgataatatg tttgattcaa tccagacatc atttgataag gcaagtgcag ggctgacttc
     1321 tcaagtcaga gaatcaattg aaaaatttgc tctatccatc aacgagcagt tacatgcttt
     1381 tgagcaagca actgaacgtg aaatgaaccg tgaaatgcaa tcattaggta atgctctgct
     1441 ttcaatcagc aaaggttttg tcggtaacta tgaaaaactt attaaagatt accaaatagt
     1501 tatggggcag ttacaaacat taatttctgc taataaacat cgcgggtaat cgatcatgga
     1561 taagattata gggaaacaat tacctaaaaa agatcaagat aatgaacatt gggtatccat
     1621 gtcagaccta atggcagggc tgatgatggt ttttatgttc atatctattg cttatatgca
     1681 ctacgtacgt attgaaaaag aaaaaattaa agaagttgcc gtagcctacg agaatgctca
     1741 gttacagctt tataatgctc tggatattga gtttgcaaag gatttacaag actgggatgc
     1801 agagatcgat aaacagactc tggaggttcg atttaaatca ccggatgttt tatttggctt
     1861 aggaagcaca gagctaaaac caaagtttaa actcattctt gacgacttct ttcctcgcta
     1921 cctaaaagtt ctagataatt atcaggaaca tattactgaa gtccgcattg aaggtcacac
     1981 aagtactgac tggacaggaa caacgaatcc tgatattgct tattttaata atatggcact
     2041 atcgcaaggt cgtacacgtg cagtattaca atacgtttat gacataaaaa atatcgcgac
     2101 acaccaacaa tgggttaaaa gtaaatttgc cgcagtaggt tattcatctg cacatcccat
     2161 tcttgataaa accggcaagg aggatcctaa tcgctctcgt cgtgtcacct tcaaagttgt
     2221 aacaaatgcc gagttgcaga ttagaaagat tattcaggag taagagatga aattatctat
     2281 cgacctttca gaacttattc aattagggaa gaaaatgtta ccagaaggag tcgatttttt
     2341 tctggatgaa tcccctattg actttgatcc tatagatatt gagttatcca cgggtaaaga
     2401 agttagtatc gaagatcttg accctggtag cgggcttatc tcttatcatg gccgccaggt
     2461 tcttttatat attcgggacc attcagggcg ttatgatgcg gctatcgtag atggcgaaaa
     2521 aggaaaacgt tttcatattg cctggtgcag aactcttgat gaaatgcgcc ataaaaatcg
     2581 atttgaaagg tatcatgcaa ctaaccgcat agatggttta ttcgaaattg atgatggttc
     2641 aggtcggagc caggatgttg atttacgggt atgtatgaat tgcctcgaac gacttaatta
     2701 taaaggaagt attgataaac aacgaaaaag agagattttt aaatcattct cattaaatga
     2761 gtttttttca gattatagta cctgttttcg tcatatgcct aagggtatct atgacaaaac
     2821 aaatagtggg tatgtcgaaa actggaagga aatatctaaa gaaatacgag aaaaggccaa
     2881 ttatgtttgt aatgattgtg gcgtgaattt atcaaccgcc aaaaacttgt gccatgtcca
     2941 tcataaaaat ggcatcaaat atgataatca ccatgaaaac cttcttgttc tgtgcaagga
     3001 ttgccatcga aaacagcccc tccacgaagg tatattcgtt acccaagctg agatggctat
     3061 cattcaacgt ttacgttccc aacaagggct attaaaagca gaatcctgga atgaaatata
     3121 tgacctgact gatccatcag tgcatggtga tattaatatg atgcaacata aaggctttca
     3181 acctcctgtt cctgggttag atcttcaaaa ctcagaacat gaaattattg caaccgtaga
     3241 agctgcatgg ccaggcctta aaattgcagt taaccttact cccgccgaag tcgaaggatg
     3301 gagaatatat accgtgggtg agctggttaa agaaatacaa accggagcct ttacgccagc
     3361 aactttgtaa attctaacac tccgtgaaag ttaagacttt cacggaagat aaataaaact
     3421 tccctgattt gtgactcaaa ttacaaaagt agtttatggc ataacttgtc tatctttatg
     3481 gtgtaatagg tataaaggta tatgctatgg ttcgcctcat acttaaaact tccctcatac
     3541 gggtgaaggt taaagctttg tagacaggag acagtcacaa tgaataaaac aataaattga
     3601 tgtcgcacgc gccgatgggc atggcgggta atgtaacttt tgtacacaac ggcaaggctt
     3661 atgttactgg cggtgttaac cagaacatct tcaatggcta ttttgaagat ctcaacgatg
     3721 ctggagaata tttagcagct atagacaaga tcaatgccta ttatttcgac aaaaaagcag
     3781 aagatcattt cttcaataag tttttgttgt cttttgatcc ctcaacacag caatggatct
     3841 tcacttgcag gcttgaacag cagaaaattt gcgagcatga tcgcaattat ttcactgcca
     3901 gggatatcaa tggcttccag taaatcgcta cagcaggcaa ttgccaacat aaaaatctgg
     3961 cacaagggtg aacagcgcgc gccgcataag ccattgttat tgctatacgt attagcggga
     4021 tacctgaatg gacatccacg ccttttcgat tatggatcgg aaatctacga acctctgcac
     4081 agtttactgg aacgttttgg gccacagcgt tcacagtacc gacctgatat gccgttctgg
     4141 cgattacaag gcgacggatt ctggcaatta cacaatgccg agctttgctc aacagcaggg
     4201 agcagccgac aaccgccagt gaaggaactg aacgaatacc acgttgcagg cggtttcgat
     4261 gaacagcact acgccctggt aaccggcaat aaaaaactca tcaatactct ggcgcaacag
     4321 atactcgaag cgcacttcac cgagagtatt caggaagaaa ttgctgacga gctgggtttt
     4381 gatctccagc aaatccgtaa acagcgcgac ccgttatttc gcaaaaatgt attacgcgct
     4441 tataactacc agtgcgcgat atgtggcttt aatatgcgcc acgacgacac caccgtcgcg
     4501 ctggaagcgg cgcatataaa atggaaacag cacggcggtc cttgtgaaat tcctaatggc
     4561 ctggcgcttt gcgcaattca ccataaagcc tttgataaag gatcgattgg cctggatgaa
     4621 aatatgcggg tactggtctc ggatgcggta aacggcggag ggatcgttga gagattattc
     4681 tgggattttg acgggaaaac tattgcgttg ccgcaggtgc gtaaaaatta cccttatgag
     4741 gggtttgtgg agtggcatcg ggaagaggtt tttagggggt gaggttgata actatcgcag
     4801 tatgttcaat attgctgaca taatcagtgc aggatcttgc cgctagttta caactcgcct
     4861 tcgatagcgg cgcaaaacgt gttctgttgc cgatatcctc ggctatggat attccaacag
     4921 ttccggcaga gttgtttacc aagtttcagg taagttttta ctcagaaccg gttgatgcgg
     4981 tttataaggc gctggatgtg aattaacaca ttcctcagct tatttacaaa aggattattc
     5041 ccatttgata taatatcagt gccaatttac t
//
LOCUS       DAPGRN010000212         4842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPGRN010000212 DAPGRN010000000
VERSION     DAPGRN010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.216"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..555
                     /locus_tag="RHW33_004602"
     CDS             <1..555
                     /locus_tag="RHW33_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein VgrG"
                     /protein_id="HDV1648026.1"
                     /translation="ETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIG
                     LVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKT
                     KKDDTGQTAIYSAGEHLELCCGKAKLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINW
                     NCAASKSPPKTPDEKQDTPDMREY"
     gene            622..4827
                     /locus_tag="RHW33_004603"
     CDS             622..4827
                     /locus_tag="RHW33_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1648027.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQLHDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPENLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYDL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGE
                     TVRYRYDDAYSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRRYDNRGRLISVKDAQGHETRYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSQSEFTYDALDRLVQQGGFDGRTQRYR
                     YDLTGKLTQSEDEGLVILWYYDESDRITHRTVNGEPAEQWQYDGHGWLREISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGNNAYELTSTYTPAGQLQSQHLNS
                     LVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGN
                     RLPDPELHPDSTLTVWPDNRITEDAHYLYRYDEYGRLTEKTDRIPAGVIRTDDERTHH
                     YHYDSQHRLVFYTRIQHEEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEE
                     TWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLIRIETENGEREKMQRRSLAEKLQQEG
                     SEDGHGVVFPAELVRMLDRLEEEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPE
                     RKIHLYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDE
                     ESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPVQHVDPLGLSTMIIGNGP
                     VPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDEEEQCM
                     MNKAIDLNKKLKTINLGPLPVLNNCFSRTNMIFEACGFNNPSMNTNAPISLQVLGELY
                     GYQRYIMPKGPFTGFPFEFQEVK"
BASE COUNT         1139 a         1286 c         1570 g          847 t
ORIGIN      
        1 gagaccattg gcaacaatca gatgattgcg gttaccaaca atcagataca gacggtgggc
       61 gttaaccaga tagagacggt gggcagtaac cagatcatta acgtgggttc tgttcaggtg
      121 gaaacgattg gacttgttcg tgcgctgacc gtgggcgtgg cgtaccagac gacggtaggt
      181 ggcattatga acacttcggt ggctctgatg cagtcctcgc agatcggttt gcataaatct
      241 ttgagggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      301 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggag
      361 ctctgctgtg gtaaggcaaa gctggtgctg acaaaggacg gtcaaatttt tctcaacggc
      421 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      481 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      541 atgagagagt actgatcccg tacctgttcg gtattcagta acaaacaagc attaaaaata
      601 aacagaagaa ggaattgaat catgagcgga aaaccagcgg cgcgtcaggg agatatgact
      661 cagtatggcg gtcccattgt ccagggttcg gcaggtgtaa gaattggcgc gcccaccggc
      721 gtggcgtgct cggtgtgtcc gggcgggatg acctcgggca acccggtaaa tccgctgctg
      781 ggggcgaagg tgctgcccgg cgagacggac cttgcgctgc ccggcccgct gccgttcatt
      841 ctctcccgca cctacagcag ctaccggacg aagacgcctg cgccggtggg gatttttggc
      901 cccggctgga aagcgccttc tgatatccgc ttacagctac atgatgacgg actgatactc
      961 aacgacaacg gcgggcggag cattcacttt gagccgctgc tgccggggga ggcggtgtac
     1021 agccgcagcg agtcaatgtg gctggtgcgc ggtggtaagg cagcgcagcc ggacggccac
     1081 acgctggcgc ggctgtgggg ggcgctgccg ccggatatcc ggttaagccc gcatctttac
     1141 ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggtggtcaga gcgggtgccg
     1201 ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac cgggctggca
     1261 gaccgtttcg ggcggacgct gacgtaccgg cgtgaggccg caggcgacct ggccggggaa
     1321 atcaccggcg tgacggacgg tgccgggcgt gagttccgtc tggtactgac cacacaggca
     1381 cagcgggcgg aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc
     1441 tcagcctcag cgttccccga cacactgccc ggtaccgaat acggccccga caggggtatc
     1501 cgtctttcag cggtgtggct gatgcacgac ccggcatacc cggagaatct gcccggtgcg
     1561 ccactggcgc ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc
     1621 aatacgcagg tgcgcgcttt cacgtatgac gcgcagcacc cgggccggat ggtggcgcac
     1681 cgttacgcgg gaaggccgga gatgcgctac cgctacgacg ataccgggcg ggtggtggag
     1741 cagctgaacc cggcaggcct gagttaccgc taccagtatg agcaggaccg catcaccgtc
     1801 acggacagcc tgaaccggcg tgaggtgctg catacagaag gcggggccgg gctgaagcgg
     1861 gtggtgaaaa aagaactggc ggacggcagc gtcacgcaca gcgggtatga cgcggcagga
     1921 aggctcacgg cgcagacgga cgcggcggga cggcggacag agtacgatct gaatgtggtg
     1981 tccggcgata tcacggacat caccacaccg gacgggcggg agacgaaatt ttactataac
     2041 gacgggaacc agctgacggc ggtggtgtcc ccggacgggc tggagagccg ccgggcatat
     2101 gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt acgctaccgc
     2161 tacgatgatg cgtacagtga gttaccggcg acgacaacag atgcgacggg cagcacccgg
     2221 cagatgacct ggagccgcta cggtcagttg ctggcgttca ccgactgctc gggctaccag
     2281 acccgctatg aatacgaccg cttcggccag atgacggcgg tccaccgcga ggaaggcatc
     2341 agcctttacc gccgctatga caaccgtggc cggttaatct cggtgaaaga cgcacagggc
     2401 catgaaacgc ggtatgagta caacgccgca ggcgacctga ctgccgtcat taccccggac
     2461 ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cggtcagcac cacgcagggc
     2521 gggctgacgc gcagtatgga gtacgatgct gccggacgtg tcatcagcct gaccaacgag
     2581 aacggcagcc agagtgagtt tacctacgat gcgctggacc gcctggtaca gcagggcggc
     2641 tttgacgggc ggacgcagcg ttaccgttat gacctgaccg gaaaactcac gcagagtgaa
     2701 gatgaggggc ttgtcatcct ctggtactac gatgaatcgg accgtatcac tcaccgcacg
     2761 gtgaacggcg aaccggcaga gcagtggcag tatgatggcc acggctggct gagagaaatc
     2821 agccacctga gcgaaggcca ccgtgttgcc gtccactatg gctatgacga taaaggccgc
     2881 ctgaccggcg aacgccagac ggtggagaac ccggagacgg gggaactgct gtggcagcat
     2941 gagacgaaac acgcatacaa cgagcagggg ctggcaaacc gcgtcacgcc ggacagcctg
     3001 ccgccggtgg agtggctgac gtatggcagc ggttacctgg cgggaatgaa gctgggcggg
     3061 acgccgctgg tcgagtatac gcgggacagg ctgcaccggg agacggtgcg cagcttcggc
     3121 aataatgcat atgaactgac cagcacttac acccccgcag gccagttaca gagccagcac
     3181 ctgaacagcc tggtgtatga ccgtgactac gggtggaatg acaacggcga cctggtgcgc
     3241 atcagcggtc cgcgacagac gcgggaatac ggctacagcg ccacgggcag gctggagagt
     3301 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccacgga cccggcgggc
     3361 aaccggctgc cggacccgga gctgcacccg gacagtacac tcacagtgtg gccggataac
     3421 cgaatcaccg aagacgcgca ctacctgtac cgttacgatg aatacggcag gctgacggag
     3481 aagacggacc gcatcccggc gggtgtgata cggacggacg acgagcggac ccaccactac
     3541 cactacgaca gccagcaccg cctggtgttc tacacgcgga tacagcatga ggagccactg
     3601 gtcgagagcc gctacctcta cgacccgctg ggacggcgaa tggcaaaacg ggtctggcgg
     3661 cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta aaccggagga gacatggtac
     3721 gggtgggacg gcgacaggct gacgacggtg cagactgaca ccacacgtat ccagacggtc
     3781 taccagccgg ggagcttcac gccgctcatc cgtatcgaga cagagaacgg cgaacgggag
     3841 aaaatgcagc gccgcagcct ggcggagaag ctgcagcagg aaggaagtga ggacggtcac
     3901 ggtgtggtgt tcccggctga gctggtgagg atgctggaca ggctggagga agaaatccgg
     3961 gcaaactgcg tgagcagtga aagccgccag tggcttgcgc agtgcgggct gacggtggag
     4021 cggctggcgg cacagataga gccggtttat ttgcctgagc gaaaaataca tctttatcac
     4081 tgtgaccacc ggggcctgcc gctggcgctt atcagtgaag acggcaatac ggcgtggcgc
     4141 ggggagtatg atgaatgggg caaccagctt aatgaagaga acccgtatta cctgcaccag
     4201 ccataccgtc tgccggggca gcagcatgat gaggaatcag ggctgtacta taaccggaac
     4261 cggtactatg acccgctaca ggggaggtat attacacaag accccattgg gctggagggg
     4321 ggatggaacc tgtaccaata tccgctgaat ccagtgcaac acgttgatcc attagggctt
     4381 agtactatga taataggtaa cggccccgtt cctgataatc cttttgggca tgctgcggcg
     4441 gcaaatcgat atggattaat gagttcggga actggggatg agatgggagc ctctgtctct
     4501 gattatttta aaaagatgca accccgcaga gatacctgga tttggataat agatacaaca
     4561 gatgaagaag aacaatgtat gatgaataag gcaatagacc ttaataaaaa actaaaaact
     4621 attaacctcg ggcctcttcc tgttttaaat aattgtttta gccgaacgaa catgattttt
     4681 gaggcgtgcg ggtttaacaa cccctctatg aatacaaatg cgcctataag cctacaagtg
     4741 ctcggtgagc tttatggata tcaaagatat attatgccta aaggtccttt cactggattc
     4801 ccatttgaat ttcaagaggt aaagtaaatg gaaatgcttg gg
//
LOCUS       DAPGRN010000213         4749 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPGRN010000213 DAPGRN010000000
VERSION     DAPGRN010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.217"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1032
                     /locus_tag="RHW33_004604"
     CDS             <1..1032
                     /locus_tag="RHW33_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDV1648028.1"
                     /translation="LSNADLLKNALFESASTHNKKSEQIESAWSQIEDMVGVRRLDKF
                     LTLHKLSEKKDRDRVLQKGFEAFIENLQQQFDGDAIAMSLMLVNSAKNYTKILENDFE
                     HPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLK
                     KQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNL
                     IKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGN
                     LTLISGSKNSEAQHYDFIKKKSIYEKLNSKSSFDLTKDVCNSSEWGLAELKMRHEKMK
                     TQLKKLWLV"
     gene            complement(1101..2057)
                     /gene="yjiA"
                     /locus_tag="RHW33_004605"
     CDS             complement(1101..2057)
                     /gene="yjiA"
                     /locus_tag="RHW33_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV1648029.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(2068..2271)
                     /locus_tag="RHW33_004606"
     CDS             complement(2068..2271)
                     /locus_tag="RHW33_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV1648030.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            complement(2321..4471)
                     /gene="btsT"
                     /locus_tag="RHW33_004607"
     CDS             complement(2321..4471)
                     /gene="btsT"
                     /locus_tag="RHW33_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV1648031.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
BASE COUNT         1273 a         1153 c         1202 g         1121 t
ORIGIN      
        1 ttaagtaatg cagatctttt aaaaaatgca ctgtttgaat cagcttctac gcataataaa
       61 aaatcagagc aaatagaatc agcatggtcg caaattgaag atatggttgg agtgagacgg
      121 cttgataaat ttcttactct tcataagctt tccgagaaaa aagatcgtga cagggtgtta
      181 caaaaaggtt ttgaagcttt tattgaaaat ctccagcagc aatttgatgg agatgctatt
      241 gctatgtcac tgatgttggt taattctgca aaaaattata ctaagatttt agagaatgat
      301 tttgaacacc cttcaatacg acgaaaaata gcatcgctaa gtaatttagg ggtagatgag
      361 tggattcctc cggttatggc attcatgaat cgaatggctc gtacagaaga ttttaattta
      421 gatgattttt ctcaatttat taccgccttt gagaaggtat atatgcatgg gtggttaaaa
      481 aaacaaatta aaagccaaag agaaatggtt tgttattcag ctctggtagc aataaataat
      541 gatatgcctt ttgattctgt gataaatcag ataaaccaac atgcagataa ttcaggtttt
      601 atagctgctc tggatgaaga tttatatgag cctcgtccga atcaagtgaa cttaataaaa
      661 gcaattttgc ttcgcttaga tatggagcaa caagatgaga gcgtgattaa aacctatacg
      721 gggagaatca caattgagca tattcttcct caagcattag ttaatgaata ctggattaat
      781 agattccaac ctcaagagca tgtctactgg ctgcataaaa taggtaattt aacactcata
      841 agtggttcca aaaattctga agcacaacac tatgatttta tcaaaaagaa atctatttat
      901 gagaaactta atagtaaaag ctcttttgat ttaacaaaag atgtttgtaa ttcttcagaa
      961 tggggattgg ctgaactgaa aatgagacat gaaaaaatga aaacgcaatt gaagaagctt
     1021 tggttggtct gacatatcta aagagttcca ctcgtaataa tgttgcggtg gaactctcaa
     1081 aaagttgatc tttcccgaac ttacttcctc aaccccgcaa acgccgcccg aatctcttcc
     1141 tccggcaact gaatcccgat aaacaccatt gtgctatgcg gtttttcatc gccccacggg
     1201 cggtcccagt cggcgctgta gaggcgctgg acgccctgga acagcaggcg gttaggttcg
     1261 ccgtcaatcc atagcatccc tttgtaacgc agtagtttat ccgccgattc cagcagcagg
     1321 ttttccatca cccgggaaac ttcgctgata tctaccgggt aatccagttc caccacaatc
     1381 gacgaaatat cgttttgttt atccgcgata aagtggaaac gcggtttggt gctgacgacg
     1441 ttctcttcca gcataaaacc gttggtgttg aacagcaggc ccaggtcgat gtcgccgtgg
     1501 gtaactgtgt agaccggtgc acgggcgttg atgcgcgcca ggcgttcgcg cagtttttct
     1561 gcttcgcctg cgacgtcggt tttggtcagc agaatacggt cggcgtagcc aacctgggac
     1621 tgggcgatgg tgaactggtt catctgctca tcggcatgta ccgcatccac cagcgcaatc
     1681 acgccgtcca gcaggtaacg ctggcataaa atctcatggg agaaaaaggt ctgaataatc
     1741 ggcccgggat cggccatgcc ggtgcattca atcaccagac ggtcgaactg aatattgccc
     1801 ttgtcgagat tatccagcag gtcgagtagt gcgtcttcca gctcgttgga gcgcgaacag
     1861 cagatgcagc cgttggtcag cgttttgatc tgcgtggcgc ggtcgccaat cagttgatca
     1921 tcaacggaga cttcgccgaa ttcgttttca atcacggcaa tcttgtagcc gtgttgttca
     1981 ttaagaatat ggcgcagcag ggtggttttg cccgcgccta aaaaaccggt gagtagggta
     2041 actgcaatcg ggttcatgct ctctccatta acaacagcgc ataccgcctt taccatcgcc
     2101 gccgtagcgc gcgttctggc gttcgcggaa gaattcttca tagctcatgt acggcttatc
     2161 gggatggttg gtcttcatat gctcgacgta gttgtcatag tctggaatgc caatcaacat
     2221 cttcgccgcc tggccgagat atttttttgc ctgtcctaag ttaccaaaca tagttcactc
     2281 tgataagaac aaagccccgc cgaagcgggg ctaaacacgg ttagtggtgc gaagagatct
     2341 tcacgccgcc ttccgggatt ggaacgtacg gagtttcttt gtcagtacgt ttgtcgctgt
     2401 tacgcaccgc aagccaggtt ttgaaaccgt agaagatgat gctgtacacc acaatcagga
     2461 acagaatact caggcccgcg ttggtgtagt tgttcacaac gatgtggttc atgttggcaa
     2521 tctgctgcgc cgtcaggtca gtaccgttag caatcttctc tttgtactgg cttgccatgt
     2581 agaagaagcc ttccatctgc gggttggtgc tgaacagttt caggcccagc gcccaggtgg
     2641 tgcagataag cagccataca gccggaacaa cagttaccca gatgtattgg gtgcgcttca
     2701 tcttaatcag cacaacggtg cccagtacca gcgctacggc tgccagcatc tggttggaga
     2761 taccgaacag cggccacagg ctcttaacgc cgcccagcgg atcgaccacg ccctgataca
     2821 gcaggtagcc ccacagaccc acacagcccg cagtaccgat gataccggca accagagagt
     2881 cggttttttt caggaacggg atgaagttac ccagcagatc ttgcagcata aagcggccag
     2941 aacgggtacc cgcatccagc gcggtcagga tgaacagggc ttcgaacaga ataccgaagt
     3001 gataccagaa gcccatgtca gccatcggca gcactttgtg gaacacatga gcgataccta
     3061 ccgccagcgt cggcgcacca cctgcacggt tcaggacaga aggctcacca atgtctttcg
     3121 cggtttgcag gatctgctct ggcgaaatca cgaagcccca ggagctgacg gtcgccgccg
     3181 cgtgtgcggt aacgtctttc aactgcgcca tgatgatcgg cgcgttctcg ccacccattt
     3241 catgcaggtt aggcatggtg atgccaaggc cagcaggcgg ggtattcatc gcgaagtaaa
     3301 gacccggttc gatgatggac gcagcaacca gcgccataat cgccacgaag gactccatca
     3361 gcattgcgcc gtagccaatg aaacgcgcgt cggtttcgtt agccagcagt ttcggcgtcg
     3421 taccggaaga gatcagcgcg tggaagccag ataccgcacc acaggcgatg gtgatgaaca
     3481 ggaacgggaa cagagcgcct ttccacagcg ggccagtacc gtcaatgtac tgggtcatcg
     3541 caggcatttt cagttccggg ttcagcacca caatacccag cgccaggccg acgataacgc
     3601 cgattttcag gaaggttgcc agatagtcgc gcggtgcgag gatcagccac accggcagca
     3661 gtgcggaaac aaacgcatag ccaatcagcg cgaaggtaat ggtggtgtct ttaaaggtca
     3721 gtgccggacc ccagtacgga tcgtgagcaa tcacgccacc gaagtagata gaggcaacca
     3781 gcagcacgat accaataaca gagacttcac ccacacgccc cggacggata aagcgcatgt
     3841 agatacccat aaacagcgca atcggtacgg ttgagcaaac ggtgaagaca ccccacggac
     3901 tttcggccag ggctttaacc acaatcagcg ccaggacggc gaggatgatg atcatgatta
     3961 agaaacagcc aaacagtgcg atagtccccg gtactggccc catctcttct ttgatcatct
     4021 caccgagaga tgcgccgtta cggcgagagg agataaacag caccataaag tcctgtaccg
     4081 caccggccag cactaccccc gccagcagcc acagtgtgcc aggcaggtag cccatctgcg
     4141 cggcgagaac cggaccaacc agcggaccag caccggcgat agcggcgaag tggtgaccaa
     4201 acaacacgta acggttggtc ggaacgtagt tcagaccgtc gttgttaata accgcaggcg
     4261 tcgcgcgcgt ggggtcgagt ttcatcacct tctgggcgat atacaaactg tagtagcgat
     4321 acgccaccag atacaccgac acagaggcga ccacgatcca cagggcactg acgtgctccc
     4381 cccgacgtaa tgcaactacc gcgaggcaga atgcaccgat gattccgaga atcacccagg
     4441 gtatgtgctt gaatagtttt ttcgtatcca tagtaaaacc tggcatgtat tgattaatag
     4501 ttggccgaag ccgttctagg tttgcgttgc gtttgaggag gtaaattgac cgctatcctg
     4561 cagtgatgct gccagagtta cgcgcgcgta aagtaaggta aataactgag tggttatttt
     4621 agggacgtaa gcggtcagtt ttgcggttga gcggttttga gcaaggttta tgaaggttat
     4681 ttatgtgatg atgatcacaa ccgaagcgat ttttagccct cggttgtgta actcatttaa
     4741 aaattatat
//
LOCUS       DAPGRN010000214         4679 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPGRN010000214 DAPGRN010000000
VERSION     DAPGRN010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.218"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..347
                     /locus_tag="RHW33_004608"
     CDS             <1..347
                     /locus_tag="RHW33_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HDV1648032.1"
                     /translation="SRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVI
                     WMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDK
                     VIGHYLNIKHYQ"
     gene            complement(527..1040)
                     /locus_tag="RHW33_004609"
                     /pseudo
     CDS             complement(527..1040)
                     /locus_tag="RHW33_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(1101..1205)
                     /locus_tag="RHW33_004610"
                     /pseudo
     CDS             complement(1101..1205)
                     /locus_tag="RHW33_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1207..1530)
                     /locus_tag="RHW33_004611"
                     /pseudo
     CDS             complement(1207..1530)
                     /locus_tag="RHW33_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2273..3250
                     /locus_tag="RHW33_004612"
     CDS             2273..3250
                     /locus_tag="RHW33_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV1648033.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3529..4269)
                     /locus_tag="RHW33_004613"
     CDS             complement(3529..4269)
                     /locus_tag="RHW33_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV1648034.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(4390..4578)
                     /locus_tag="RHW33_004614"
     CDS             complement(4390..4578)
                     /locus_tag="RHW33_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV1648035.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT         1125 a         1178 c         1170 g         1206 t
ORIGIN      
        1 tgtcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
       61 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
      121 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
      181 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
      241 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
      301 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
      361 cctgcttttt cggttgaaaa gaaattgatt gaattactgg aaaaacaatc tggtctccat
      421 acgtctgtgg attgcagtac tccccttaca gagcagagtc tgtcgagaaa tgacggtgaa
      481 gaccagacca gaagaaacgc tgaaaatatg ttcggagatg actgaactaa cccagcctgg
      541 ccaggggaat atctcgccat ttagttgtat aacactgact caacatttcc cgtttcattt
      601 gccattcagg ggcggttcct cgccccgcaa accacacttt cccttttccc gactggttca
      661 gttcatcgag tgttttcatt aacttttcac tgtttttacg gggctggatt tcatcaaata
      721 atcccggctg cgctataccc gatggtgtga aatcagccag catgactcct gccttcatat
      781 agcggtaccc ttcacgccag acatggttta aggctctgca tgcggcggca ataatgtccc
      841 ggctgtcctg tgtgggcaat gaaagctttt ccacagcggc attgctgtaa cagggttcag
      901 ggggatgtcc gtacaaatgt cgtcacctgc cggcaatact gacgctcccc acgtagtttc
      961 tctgcggccc gctctgcata ctgaacaaca gcctggtgca tggcatcttt gtctgtgatt
     1021 cgttcaccaa aactgcgact atgtcaacgc cacgatgttt gaccgttatt tgccattttc
     1081 atgcccgcta aatactttcg ttactgatgc tgtccgcttt gcaccatttc atcatcactg
     1141 attggcgttg tgtttttggt cagcactcca gctttacgtt tctcttttaa ccggtaactc
     1201 tctccttgtt ctttttgacg gacgcatttt acgtttgggc tggatgtagt aacgcagcat
     1261 ggaacgtccg cccgtataac ccattgcctt aatctccgcg aagataacct cgctattcca
     1321 gacattctct gccaggcgca tgtcgatgta atccataaac ggtttcagct taaccatttt
     1381 gtggcgggtc tttctggctg gcggttcagg gtatttgagg tagcgtctga ccgtccgttc
     1441 agagcaacca atctgagccg caatatcgac aatgtacgcg ccctgctggc gcatttgctt
     1501 tatcatgtaa aagtcctctc tgctcagcat gttgatgtcc tttctggtgt gagaacctta
     1561 aggaaacaac atgttgggtg gagcggacaa tccaaatggt gaattaccgt cttatatcac
     1621 tggcgctgac acctgtagtt tgtatacaca atccctggca ttgaaacaca catatgaagt
     1681 gtacttcgga tatgaccgta cgctcaggaa gtacggcaga ttccgtcaaa aattacctgt
     1741 aaagacagac atcagaaaaa acagtgctcg ccgcagaccg gtgaccgggc gaagcccgcg
     1801 aaccggcgag gaagcgcaat ggatctgtag ttacagtgag caggtacgcc ctgaagcctt
     1861 cataatcggt agtgtatgct gtttttctgg aatgatttat ccacatatcc agaggcccga
     1921 tccagtaaac agatccatga atgatcaaca aaggatccat taaagatccc catatcgctg
     1981 tcaaccttgt cactcatggg ccgggaccac gttcacataa gcggtggcat gttatggata
     2041 aactgtaaca ggttcatgat aagctgtatt cagtaatgca tatactgaag taagttaatg
     2101 acataaacta tggtcagtac gccagactca gttgttaaat acagactgca ggtttttctt
     2161 cagtcagttg acggggttct gacacacgat tttctgttta ttcttttact gtccacaggc
     2221 aggaggcttt ctggaaaacg aaaattcaga catcaaaaaa ctgttcggcg aggtggataa
     2281 gtcgtccggt gagctggtga cactgacacc aaacaataac aacaccgtac aacctgtggc
     2341 gctgatgcgt ctgggcgtct ttgtaccgac ccttaagtca ctgaagaaca gtaaaaaaaa
     2401 tacactgtca cgcaccgatg ccacggaaga actgacacgt ctttccctgg cccgtgcaga
     2461 aggattcgat aaggttgaga tcaccggccc ccggctggat atggataacg atttcaagac
     2521 ctgggtgggg atcattcatt cctttgcccg ccataacgtg attggtgaca aagttgaact
     2581 gccttttgtt gagtttgcaa aactgtgtgg tataccttca agccagtcat cccgcaggct
     2641 gcgtgagcgc atcagccctt ccctgaagcg cattgccggt accgtgatct cgttttcccg
     2701 taccgatgag aagcacaccc gggaatacat cacccatctg gtacagtcag cctactacga
     2761 tactgagcgg gatattgtgc agttacaggc cgatccccgc ctgtttgaac tgtaccagtt
     2821 tgacagaaaa gtccttctcc agcttaaggc gattaatgcc ctgaagcgac gggagtccgc
     2881 ccaggcactc tacaccttta tagagagcct gccccgggat ccggcaccga tatcgctggc
     2941 gcggctgcgt gcacgcctca atctgaagtc tcctgtattt tcccagaacc agacggtcag
     3001 acgggcaatg gagcagctgc gcgagattgg atatcttgat tacacggaga tccagcgggg
     3061 gcggacaaaa ctcttctgca ttcactaccg gcgtccccgg ttaaaagcac cgaatgatga
     3121 gagtaaggaa aatccgttgc caccttcacc tgcggaaaaa gtcagtccgg agatggcgga
     3181 gaagcttgcc ctgcttgaaa aactgggcat cacgctggat gacctggaaa aactcttcaa
     3241 atcccgctga acataaactg tagtcagtga agagtgctcc tttactgact acagcttata
     3301 ttatcaggtg cagtgagtgg tctgctcact gcagtttata ttcagtttca tgcagtgctg
     3361 cctgtggctg agctgccatc tgcctgtccc ttacgtgagt caccccgtaa cctgatgctg
     3421 aggcattgct cccttcataa aacatggttt actcactaca gcttatctac ctgctccagc
     3481 ttatgttatg tctgttctgc tgaccactgc ttatataagg aggtgccgtt acttcagggg
     3541 aggcagtgta cgcaggatct ccgcagcatc ccgcccgtca cctgtgaaag gcactgccag
     3601 cgtggctgcc atatccagtg caaacactct ggtataaacc tccatagaac gtggatccct
     3661 gtgaccagcc agtgcctgga tgactttccg gggctggcgg tgatagagca tgtgcatgat
     3721 atagctgtgc cggaaggtgt gtggtgtgac cggaatcgaa aagtgtactc cgtcagcttc
     3781 ggcccgtctg acagcctgct tcagccagtt gcgcatggtt tcgtcggtca cggcccataa
     3841 tggttcacga cgacggggcc gggtggtgat catccagctt tccatctgcc tgacatagct
     3901 tatatccgtc agcggaacca ggcggacttc atcttttggc gggcgtccgc gtcgcgcacg
     3961 cactttttcg gacaggatcc gcacaaacgg tcttactcca tccaggtcaa atgattccgg
     4021 cgtcagcatc cgggcttcgc caatacgcat tccggtattc cagagggtgg cgaacagcat
     4081 atggtgacgc tgatccggca tatagaaaag aagggcactc acttccgggg ccagcaggta
     4141 ggccggtgtg gcccctgtgg aagtggccat tcttctcagg gagagcgctg ttgcaaaatc
     4201 cacccccggc gcaatgggta acagggagac gcgttctggt gaattctgca ggggaatgac
     4261 attgttcatg agttattttt cacgtagcac acgcactgtt tatggataca ggtattctga
     4321 cctgaacgcc cggatccttc cacgacaata aatcgcagcg cgcggatcct tttgtttgct
     4381 tatcacgcat tattgcgggt aaaaccaaat acgtaaaggc gcgttttgcc gcagggatcc
     4441 tgtcttaagc cagtcatggt gcgggctgag gactggtttg ccgtttttgg gagaattggg
     4501 gtaatgccag gaacaggcag atacggagaa aaatcctgca gataaccttc tacgacaact
     4561 gaaggtgtct gacgccatat tcttagctac ttctcagttt taaggcggct tagtcacaac
     4621 ataaggttat gcataataag catattttat tatgttatgt tatggtcttg ttcatgcat
//
LOCUS       DAPGRN010000215         4587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.219, whole genome shotgun sequence.
ACCESSION   DAPGRN010000215 DAPGRN010000000
VERSION     DAPGRN010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.219"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(35..454)
                     /locus_tag="RHW33_004615"
     CDS             complement(35..454)
                     /locus_tag="RHW33_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV1648036.1"
                     /translation="MKKIQIVCSGIVLVVISSLAQAVELSLNTSDGRSGELKDGTKVA
                     TGRIICRGTYTSFHIWMNSRQMGNIPGHYIILGRHDSHNEMRVRLDGAGWLPSVSDGQ
                     GMVSTGIPEQHTFDVVIDGNQLLGPDEYILSVSGECS"
     gene            complement(470..3058)
                     /locus_tag="RHW33_004616"
     CDS             complement(470..3058)
                     /locus_tag="RHW33_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110996819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV1648037.1"
                     /translation="MHRNKSSSGLSLYCVKTKITAVALSVMAACTLLYTGNTEARTYS
                     FDASMLKGGGKGVDLTLFEEGAQLPGIYPVDIILNGSRVDSRDMAFRTEKDAEGKTYL
                     KTCLTREMLARYGVMTEEYPELFHGDDGKEAEACAELSVIPQATETYQFASQQLLLSI
                     PQVALRPPLRGIAPEALWDDGIPAFLLNWQANTSRSEYRGYGKSVTDNYWVTLEPGIN
                     LGPWRVRNLTTWNRSSGQPGQWESAYIRAERGINSLRSRLTLGEDYTPSDIFDSVPFR
                     GVMLGADESMVPYNQREFAPVVRGIARTQARIEVRQNGYLIDSRTVAPGAFALTDLPL
                     TGSGGDLQVTVQESDGTAQVFTVPYTTPAIALREGYMKYSIAGGEYRSSDDAVEHSPL
                     GQMSVMYGLPWGLTAFGGAQMSSHYQSAALGLGWSMGRLGAVSVDGIHSRGQQKGRDT
                     ETGETWRLRYNKSFELTNTDLTAARYQYTSSGFHTLSDVLSTFRNDNFRAYSYSGDRS
                     RRTTLRLNQSLGSLGYMSFYGSRDEFRHNRQKQDSFGMSYGTSWKNISWYVNWSRNYS
                     TNAYYQRGHIEDSINLWMNIPLGQWIGGRDNDINVTTQTQRTTGQNTWYETGLNGRAF
                     DRRLYWDVSERIAPGSENNVDSSRLNLRWFGTYGELAGMYSYSSYIRQMSAGISGGMV
                     LHNEGITLGQKGEDTVALVVAPGVNGASVGSLPGVRTDTRGYTLVNHVSPYQENLITL
                     DPTTFPENVEVSQTDTRVVPTKGAVVRAKFITRVGGRALITLSRSDGSRLPFGAVVTE
                     EGKKGQMPGGAGVVGDNGEVYLSGLDETGQLKVQWGRNSSCRANYRLPEEKEATGIFL
                     VRTVCM"
     gene            complement(3227..3994)
                     /locus_tag="RHW33_004617"
     CDS             complement(3227..3994)
                     /locus_tag="RHW33_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1648038.1"
                     /translation="MKIRHLAALVGILAGLPASGVMAAGNQESTKTTSKVFSLHLGAT
                     RVIYNLDSSGASLTVMNDLDYPMLVQSEVLAENQKDPAPFVVTPPLFRLDALQSSRLR
                     IVRTGGVFPEDRESLQWLCVKGIPPKHDDRWAEEKGADKKKADKATLQVNLSVSSCVK
                     LFVRPPAVKGPPEDAGGKVEWTKSGNKLKGNNPTPFYINISELSVGGKEVKEHRYISP
                     YSSYEYEIPSGASGKVSWTVITDYGGKSKPFESELKS"
     gene            complement(4072..4416)
                     /locus_tag="RHW33_004618"
     CDS             complement(4072..4416)
                     /locus_tag="RHW33_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HDV1648039.1"
                     /translation="MNSEVCLHLVKERSGILLPGEVSERHFWLLIEISGMHSEKVINA
                     LREHLVMGYTRREACEQHGVSQGYLSGALVRIQHLSQTVNNLLPYYVSGLAMNHEEEG
                     DRSVKRGGDWRV"
BASE COUNT         1182 a         1372 c          874 g         1159 t
ORIGIN      
        1 tagttacttt attagcataa agaaattatg aatattatga gcattctccg ctaactgata
       61 atatatattc atcaggccca agcagctgat ttccgtcaat cacaacatca aatgtatgct
      121 gctcaggtat cccggtactg accatacctt gcccatcact taccgatggc aaccatcctg
      181 cgccatccag cctaacccgc atttcattat gactgtcatg tctacccagt ataatatagt
      241 gaccaggaat atttcccatt tgtctgctat tcatccagat atgaaaactt gtataggtgc
      301 ctcggcagat aatccttcct gttgccacct tcgtaccgtc ttttaactcg ccactccttc
      361 catcactggt attaagactc agttcaactg cctgcgccag actggatata accaccagta
      421 caataccact acatactatc tgtattttct tcatcagggt actccttggt cacatacaca
      481 cagtgcgcac cagaaaaata ccagttgctt ctttctcctc tggaagtcga taatttgcac
      541 ggcaagaact gttacgcccc cattgaacct tcaactgtcc ggtttcatcc agtccgctca
      601 aatacacttc tccattatcc cccacaaccc ctgccccacc aggcatttga cctttctttc
      661 cttcctctgt caccacagct ccaaaaggca ggcgagagcc gtcactacgt gacagagtta
      721 tcagtgctct tccccctact cgagtgataa acttcgcccg aaccacggct ccctttgtag
      781 gaacaacacg agtatctgtc tgtgatactt ccacattttc cgggaaagtt gtcggatcca
      841 gtgtaataag attttcttgg tatggagata catgattcac aagagtgtaa cctctggtat
      901 cggtcctaac gccaggcaaa ctcccaactg aagcgccatt aactccaggt gctacaacca
      961 gagcaaccgt atcctctcct ttctgaccaa gagtgatacc ttcattgtgg agcaccatac
     1021 ctcctgatat accagcactc atctggcgaa tataactgct gtaactgtac atccccgcca
     1081 actcaccata ggtaccaaac cacctcaaat tcagtcggct actgtctaca ttattttcac
     1141 ttccgggagc tatccgctca ctcacatccc aatacaacct acggtcaaat gcgcgaccat
     1201 tcaaaccggt ttcgtaccaa gtattctgcc ccgtagttct ttgcgtttga gttgtaacat
     1261 taatatcgtt atctctaccc ccaatccact gccccagagg aatattcatc cacagattga
     1321 tactatcctc tatatgccct ctctgataat atgcatttgt actatagtta cgtgaccaat
     1381 taacgtacca cgaaatattc ttccatgacg ttccatatga catgccgaaa gaatcctgct
     1441 tctgtcggtt atgacggaac tcatcacggc taccgtaaaa gctcatatac cccagagatc
     1501 ccagagactg attaaggcgt aatgttgtcc ggcgactcct gtccccacta taactatatg
     1561 cacgaaaatt atcatttcta aatgtactca gcacatcgga caatgtgtgg aatcctgagg
     1621 acgtatactg atatctcgcc gctgtcaaat ctgtattcgt aagttcaaat gacttgttgt
     1681 aacgcagacg ccatgtctct cccgtctccg tatcccgtcc cttctgctga ccgcgggaat
     1741 gaatgccgtc aaccgacacc gcccccagtc tgcccattga ccatcccagc cccagagccg
     1801 ctgactgata atgcgatgac atctgtgctc ccccgaaggc cgtcagcccc catggcagac
     1861 catacatgac actcatctgc cccagcggtg aatgctcaac agcatcatct gaagaacggt
     1921 actccccccc ggcaatgctg tacttcatat atccctcacg aagcgcaatg gccggtgtgg
     1981 tgtaaggcac cgtaaacacc tgagcagtac catcagattc ctgtaccgtt acctgaaggt
     2041 ctccacctga ccccgtcagc ggcaggtccg tcagggcaaa tgcgcccggg gccaccgtcc
     2101 ggctgtcaat cagataacca ttctggcgca cctcaatcct ggcctgggtc cgggcaatac
     2161 ctctgaccac cggcgcaaat tcacgctggt tataaggcac catgctctca tctgccccca
     2221 gcatcacccc acggaacggc acactgtcaa aaatgtctga aggcgtgtaa tcctcaccca
     2281 gcgtcagacg acttctgaga ctgttaatcc cccgctccgc acgaatatag gcactctccc
     2341 actgccccgg ctgaccggat gacctgttcc aggttgtcag gttacgcacc cgccagggac
     2401 caagattaat gcccggctca agagtgaccc agtaattgtc agtaaccgat ttcccgtatc
     2461 cacgatattc tgaacgactg gtatttgcct gccagttcag cagaaaagca ggaataccat
     2521 catcccataa ggcctccggc gctatccccc tcagtggcgg gcgcagggcc acctgaggaa
     2581 tactcagcag caactgctgt gacgcaaact ggtatgtttc cgtggcctgt ggaataacgg
     2641 ataactcagc acaggcttca gcttcctttc cgtcatcccc gtgaaacaac tccggatatt
     2701 cctccgtcat gacaccatag cgggccagca tttcccgggt cagacaggtt ttcagataag
     2761 tcttaccctc cgcatctttc tccgtacgga acgccatgtc ccgcgaatcc acccgcgaac
     2821 cgttcaggat aatatcaacc ggatagatac cgggcagttg ggcaccttct tcaaacaacg
     2881 tcaggtccac cccctttccg ccacctttaa gcatggaagc atcaaaggag tatgtgcggg
     2941 cctcagtatt cccggtgtaa agaagggtac aggccgccat cacagagaga gcaaccgctg
     3001 taatttttgt ttttacgcaa tataaagata aacctgaaga tgacttattc ctgtgcatta
     3061 ttcacacctg agccacggtt tctttttatt tacatatgcc ggaggatata cacacgtatt
     3121 tgtgcgtttg cacgcctgtt cacacacact tatttcaaac acgctcagat attcaaccgg
     3181 gttaatcaaa taagcccgcc tctgacgtta atatttattc cggctttcag ctttttaatt
     3241 ccgattcaaa cggcttactc tttccgccgt aatccgtaat cactgtccag ctgacttttc
     3301 ctgatgcacc agaaggaatt tcatattcgt aagaggagta cggagaaata taacgatgct
     3361 ccttaacctc tttcccacca acgctcagct ctgaaatatt aatataaaat ggtgtcgggt
     3421 tattcccttt cagtttatta cctgattttg tccactccac ttttccgccc gcatcctccg
     3481 gaggcccttt aaccgcaggc ggacgaacaa acagcttcac acaactgctg acggaaagat
     3541 ttacctgcag ggttgccttg tcagctttct ttttatcagc acctttctct tctgcccatc
     3601 ggtcatcatg ctttggcgga atgcctttca cacaaagcca ctgcagactc tcacggtcct
     3661 caggaaaaac acccccggta cggacaattc gcaaacggct cgactgaagt gcatccagac
     3721 ggaaaagcgg cggcgtcacc acaaaggggg ccgggtcctt ctgattctct gccagcacct
     3781 cagactgaac cagcatcgga taatccaggt cattcattac cgtcagtgag gcccccgatg
     3841 aatccaggtt gtaaatcacc cttgtggccc ccagatgcag ggaaaaaacc tttgacgtag
     3901 tcttcgtgct ttcctggttt ccggctgcca ttacgccgga agccggcaac cccgccagta
     3961 tgccaacgag cgctgcaaga tgtctgattt tcattatttt atcctgaagg caaataaacc
     4021 ttttataaat atgttaatag caaccgcccg acagctgtca ccagtatccc ctcagacccg
     4081 ccagtcacca ccacgcttta ctgaccgatc accctcctct tcatggttca ttgccaaacc
     4141 agaaacataa taaggaagca gattattcac cgtctgactg aggtgctgaa tccgaaccag
     4201 cgctcctgat aaatacccct gagaaacacc gtgctgctca caggcctccc tgcgggtata
     4261 tcccataaca agatgctccc tgagcgcatt aatcactttc tcactgtgca tcccggatat
     4321 ttcaatcaat aaccagaaat gcctttctga tacttccccg ggcagcagaa tcccggaccg
     4381 ctctttcacc agatgcaaac aaacctccga attcataacc ccgtttactc tgacagggaa
     4441 acaaactctc agcatcagcg ccggaatgaa caccggtaat cccctgcctc gccaccaaca
     4501 ttaccatgga gtttaatgct gtaaatatga ttattgacga tctttttaac caaataacaa
     4561 caattaaaca ggttataaca ctactcc
//
LOCUS       DAPGRN010000216         4527 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.220, whole genome shotgun sequence.
ACCESSION   DAPGRN010000216 DAPGRN010000000
VERSION     DAPGRN010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.220"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..302)
                     /gene="chbB"
                     /locus_tag="RHW33_004619"
     CDS             complement(<1..302)
                     /gene="chbB"
                     /locus_tag="RHW33_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV1648040.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAI"
     gene            complement(601..939)
                     /gene="osmE"
                     /locus_tag="RHW33_004620"
     CDS             complement(601..939)
                     /gene="osmE"
                     /locus_tag="RHW33_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV1648041.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1141..1968
                     /gene="nadE"
                     /locus_tag="RHW33_004621"
     CDS             1141..1968
                     /gene="nadE"
                     /locus_tag="RHW33_004621"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV1648042.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2198..3085
                     /gene="cho"
                     /locus_tag="RHW33_004622"
     CDS             2198..3085
                     /gene="cho"
                     /locus_tag="RHW33_004622"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV1648043.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSQAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3045..3620)
                     /gene="ves"
                     /locus_tag="RHW33_004623"
     CDS             complement(3045..3620)
                     /gene="ves"
                     /locus_tag="RHW33_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV1648044.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESTDRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3823..4308)
                     /gene="spy"
                     /locus_tag="RHW33_004624"
     CDS             complement(3823..4308)
                     /gene="spy"
                     /locus_tag="RHW33_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV1648045.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1136 a         1098 c         1091 g         1202 t
ORIGIN      
        1 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
       61 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
      121 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
      181 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
      241 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
      301 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
      361 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
      421 acggtataga cctgagcaat agtatttctt tgactttata aattgtctct ccttgacctg
      481 cggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcaga
      541 ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca gacgaagtga
      601 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
      661 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
      721 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
      781 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
      841 ctgtacaaac tggtctttgg tacgatcata agccgtacaa cccgccagca tggttaatac
      901 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
      961 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgtgaa cgaaacggct
     1021 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
     1081 gtttcagtag caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
     1141 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgccgaa
     1201 gaggaaattc gtcgtagtgt cgattttctg aaaagctacc tgcgaactta tccgttcatt
     1261 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg taagctgtgc
     1321 cagatggcga ttaatgagct gcgccaggaa accggcaacg aatcactgca atttattgcc
     1381 gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
     1441 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc tagcgagcag
     1501 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
     1561 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
     1621 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacggac
     1681 attaatccac tgtatcgtct caacaaacgt cagggtaaac agttactggc agcattaggt
     1741 tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga tcgcccttct
     1801 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
     1861 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
     1921 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacct
     1981 attggattgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
     2041 gacctacaaa actatgcaaa ttcaatacat tgcaggagtc gaataggcct gacaggcgta
     2101 gcacgtcaga cggtgtaacc tttgtcatcg acccgcgtct tttttaatcg cttcccgcct
     2161 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
     2221 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
     2281 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
     2341 gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt tacgcacccc
     2401 ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgca ccgcaggcga
     2461 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
     2521 acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt
     2581 ggtgtatgcc aaagaggtgg atttttcaca agcccccaac ctgtttggcc tgtttgccaa
     2641 taggcgcgca gctttgcaag cattgcagac catcgctgat gaacaaaaac tttgttatgg
     2701 cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg
     2761 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
     2821 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggggcggtgg cgctgaaaga
     2881 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
     2941 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
     3001 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
     3061 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
     3121 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcagtttg
     3181 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
     3241 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg cgtcataata
     3301 ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt ttaccacctg gtccgccgca
     3361 aaggcaaaag gttgtaacgg ttttaaagta tggttaaagc ggtctgtgct ttcaagaaac
     3421 atctcgccgc cttccagcaa cgtcactatc ctttccatac cgggaaacaa agaaaactcc
     3481 ccattagccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg
     3541 aaagtgcaaa tttcgcgcgt ttcaccggca gcgtttcgcc acagattcac cgacatttta
     3601 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
     3661 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt
     3721 tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
     3781 tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg
     3841 cattttacct tttgccgctg gacgttctgt cagacgcttc tcaaaattag cattaaattg
     3901 ctttttctgt tccggcgtca ggatgttgta aattttgttc tgggtttcca tgtgcgccag
     3961 catgttagct ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt ttgctttatc
     4021 gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt
     4081 catctggtca cgctggcctt tcatgatttc gcggatctgc tgtttttgcg cgtcggtcag
     4141 gttcaggtct ttgaacatca tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat
     4201 cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc
     4261 gccaagagcc agggtagagg caacaaacag tgcagttaat ttacgcatat tctatatcct
     4321 tcctttcagt tatttattac ggctttctta agtagcgtgc cgtgttgacg agattaactt
     4381 tactgacttt agcgtcaatt aatcagagca acggtaaaac aatgaaagtg taaaaaacac
     4441 ttttgcgcca attatggaga aaaaaagaaa atttgatgga gagtgatgag cgaatattac
     4501 aacacgatga ttttgcagag attatga
//
LOCUS       DAPGRN010000217         4514 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPGRN010000217 DAPGRN010000000
VERSION     DAPGRN010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.221"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..847
                     /gene="hyxR"
                     /locus_tag="RHW33_004625"
     CDS             53..847
                     /gene="hyxR"
                     /locus_tag="RHW33_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV1648046.1"
                     /translation="MKKPLVIISACQFTRLALKSLIPTDRYLVRVYSNVTTEVEFILK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPAASLYYGFP
                     RISTLLTASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATRALMKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVAVGESINSDAISGKVLSGQRKNMSKKRPGLL"
     gene            1001..1762
                     /gene="hyxA"
                     /locus_tag="RHW33_004626"
     CDS             1001..1762
                     /gene="hyxA"
                     /locus_tag="RHW33_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV1648047.1"
                     /translation="MRFVLFCPSGIVPAQFVALSTGSVGNVTCIKTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKPGMS
                     VEHVISIANTSGGFSGLKHAEILPVMDSYSVFMKEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..2856
                     /locus_tag="RHW33_004627"
                     /pseudo
     CDS             1804..2856
                     /locus_tag="RHW33_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            3079..4272
                     /locus_tag="RHW33_004628"
     CDS             3079..4272
                     /locus_tag="RHW33_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1648048.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSV
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVNVHSDSYNSVLGEAGTRVGYDFAVGNATVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
BASE COUNT         1241 a          979 c         1131 g         1163 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggtttcgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt caccaggctg gcactgaagt ctctcatccc
      121 aacggacagg tatctcgtgc gggtgtacag caacgtcact acagaggttg aattcatttt
      181 aaagacctcc tgtggctacc tgttagctga tatcccttcg cttccttcgg gggagttggt
      241 tcttctggct tgtcttgtcc gcttgcggag tgatgccggg caatggcagg tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgctgcaagt ctgtactacg gcttcccacg
      361 cataagcacc ctgctgacag cgagtcgtta tcagaatcaa attatccgct ggttactacg
      421 tcctttgccc ggcgatatga gtgatttctg gctgctgact gcacgggagt tgagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagaccctg catgcgattg ccacacgggc attaatgaaa cttggactgg gaactctgag
      601 cgatttcagg ctgctctata cgggatgcgg tgacagcaga gtgaaacgga atatacggct
      661 acatgcaaga tatgtcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtatccgga attctttatc cagagaaagt tgccgttggt gaatcaataa attctgacgc
      781 catatccgga aaagtacttt ccggacaaag aaaaaatatg tctaaaaaaa gaccaggatt
      841 gttatgacac agcagcgttg ttactacccc ccactgcggc gcatacggac tcgctacagt
      901 gtaggagcca aaatatttct actgctctgg gctgcaggat tgggaggcac gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga atcgtaccag cgcagttcgt agctctatca accggttctg tcggcaatgt
     1081 tacctgcatc aaaacagaag aggagttacg caataaattg cggcacaggc cacagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatcggga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccacctg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaccagga atgtcagtag agcatgtcat
     1321 cagtattgca aatacctctg gtgggttttc agggctgaaa catgcagaaa ttttgccagt
     1381 tatggacagt tactctgtct ttatgaaaga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg ttgcttcttg ccggacacag
     1501 ctgggaatac agtgcgcagt ttctcaaaac ggggataaga cagatatggc ttgccgagca
     1561 gtcgctaaaa aagcgttggg gaataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aaatttcacg gtgatggaaa tgcattggaa aaaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtggaaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaggc attttaccgc agcgcattag caacagctat tgttatggct
     1861 ctttctgccc cagcattcgc tgctgataac gcggtatcaa ctgattcggt tacgctgaat
     1921 aaagataaga cgactctgga tcaagatgtt gttattagca atacagcaga caaacagatt
     1981 acagccgtaa caattaatgc ggcagatgaa gaccttaatg ttgcttttgc tggtcatgat
     2041 attactgctg aatcaacggc agacaacaaa ttcgctgaag gtgtaaaagt tagcggtgac
     2101 aaaaatgttg tgattaatgc tacaggctcc accatcacag ctcaaggtga aggcacctat
     2161 gtccggactg caatggtcat tgattcaact ggcgatgttg ttgttaatgg cggtaatttc
     2221 gttacaaaaa atgaaaaagg tagtgcgaca gggatatctc tggaagggac caaaggaaat
     2281 aatgtaacgc tcaatggcac aaccataaat gctcaaggta ataagagttc cagcaacggc
     2341 tctacggcaa tttttgctca aaagggtagc gtattggatg ggcatggctt tagcggtgat
     2401 gcaaccgaca acattaccct tgctggctca aatattatta atggccggat tgaaacaata
     2461 cttattgctc aggagaataa gggaactcat acagtcaacc tgaatattaa ggatggctca
     2521 gtaattgggg cggctaataa taaacaaaca atttatgcct ctgcttcggc acaaggagca
     2581 ggttcagcaa cgcaaaattt aaatttatct gtcgccgatt caaccatcta ctctcatatc
     2641 caggtccttt ctgcaagcga gaattcagtc ggtaccacaa caaatgtaaa catgaacgtt
     2701 gcccgctctt actgggaagg taatgcttat gctgatgctt atggcgataa agcaagtagt
     2761 aatctgaata taaatctttc cgatggctca gtctggaaag gtaacgtagg tggagctctg
     2821 gatagtagta cacgtgtcac actgaaaaac tccagctggg atggtgatgt cggcaacgcc
     2881 aaaactgctg tttttctgac ggatggttct gtgtggaatg gtgctgtttc cattaactca
     2941 caatgggacc gcgcatcagc accaatgggc tcggttcaac cgaataaaaa cagtgtatct
     3001 ctgcaaaacg agtctgtctg gaatgttacg ggatcctcaa ctgttgatgc tctggcagta
     3061 aaagacagta cggttaatat cacgaaggct acagtcaata ctggcacgtt tgcttctcaa
     3121 aacggcactc tgattgttga tgcctcttct gaaaacactc tggatatcag cggtaaagcg
     3181 agcggtgact tgcgtgttta cagtgcgggt tcattggatc ttatcaatga acaaacggca
     3241 tttatttcta ccggcaaaga cagcactcta aaagccacag gcacaacgga aggtggtctg
     3301 tatcaatatg acctgacaca gggagctgat ggtaactttt atttcgtaaa aaacacgcat
     3361 aaagcatcca acgccagctc cgtgattcag gcaatggcag ctgctccggc taacgtcgct
     3421 aatctgcagg ctgacacgct ctccgcccgt caggatgctg tccgtctgag cgaaaatgac
     3481 aagggtggcg tatggattca gtactttggc ggtaaacaga aacataccac cgcgggaaat
     3541 gcatcctatg acctggatgt aaacggtgta atgctgggtg gtgatacccg cttcatgact
     3601 gaagatggta gctggctggc cggtgtggcg atgtcttctg cgaaaggtga catgaccacc
     3661 atgcagagca aaggcgacac tgaaggttac agcttccacg cttacctgag ccgccagtat
     3721 aacaacggta tcttcattga tactgctgca cagtttggtc actacagcaa cacggcagat
     3781 gttcgcctga tgaatggtgg cggtaccatc aaagctgact ttaacaccaa tggtttcggt
     3841 gcgatggtta aaggcggtta cacatggaaa gacggtaatg gcctgtttat tcagccatat
     3901 gccaaactgt ctgcgctgac gctggaaggt gtggattatc aactcaacgg cgtgaacgtt
     3961 cattctgaca gctataactc tgtgctgggt gaggccggta cgcgcgtggg ttatgacttc
     4021 gctgtgggca acgcgaccgt taaaccttat ctgaatctgg ccgcactgaa cgaattctct
     4081 gatggcaaca aagtccgtct gggtgatgag tctgtcaatg ccagcattga cggtgcagca
     4141 ttccgcgtgg gtgcaggtgt acaggctgat atcaccaaaa acatgggagc atatgcaagc
     4201 cttgactaca ccaaaggtga cgacattgag aatccgctac agggtgtagt tggtatcaat
     4261 gtgacctggt aagaataaag cagtcggttc ccctgtcgac tgttataccc cctgcaggca
     4321 gcagtatgct gcctgcaggc tataaatatc cagtattcat gatgctggct ttttgctgat
     4381 atttaacccg aacattcctg atacagacta ttaattcatt tatctgcggc agattttatt
     4441 ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg agattttatt
     4501 ttatctgaag aaaa
//
LOCUS       DAPGRN010000218         4220 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPGRN010000218 DAPGRN010000000
VERSION     DAPGRN010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.222"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            384..647
                     /locus_tag="RHW33_004629"
     CDS             384..647
                     /locus_tag="RHW33_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDV1648049.1"
                     /translation="MPYHKDRNLYFLSSSMVAQAGQALAWPVSDDAGIPTPVWAITNE
                     RRNSVIAFLVVIGDLCYVTSTDTETPGHHSVGRHVSRAINKPA"
     gene            565..852
                     /locus_tag="RHW33_004630"
     CDS             565..852
                     /locus_tag="RHW33_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraA"
                     /protein_id="HDV1648050.1"
                     /translation="MSPQLIQKLPAITLLEGMFPELSTNQLKVCVFYAMGVPYDAIAQ
                     NCRLSPETVRTYLKRSLKNLNLEGYDALRSAVLMRTFVFMISNTAKENEKM"
     gene            1294..1827
                     /locus_tag="RHW33_004631"
     CDS             1294..1827
                     /locus_tag="RHW33_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HDV1648051.1"
                     /translation="MNIEHLNNRNWYLAQYNTAGKNRESLFSWLNEQNVVPWTPLITR
                     KIRRADSRCCYRERIFAIFPGYFFILANFDIHPVSALRRHSAFIDFVKFGGEIKPVNK
                     DIVDGLMKIYPDPVLNPGAREELNAASSIWLTKAQYQYLLRMENTLQPESRISLLLEL
                     VSNAEHHGFMERLVNIP"
     gene            2081..2764
                     /locus_tag="RHW33_004632"
     CDS             2081..2764
                     /locus_tag="RHW33_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
                     /protein_id="HDV1648052.1"
                     /translation="MTTTEHPVRQLLWCALNALKTAQENHNITSETGLRHYLLEWLSG
                     ASRHPEFRSLPGEIMALKALTEKDRNIPIIGTLNTLFLSSATVGECPLFRFRAALGKL
                     QKAGWRTYVCPWPERVFNESIERACSGKRHLLQLSRTEECFLPTGDMRAPVTLQLITP
                     LHRKTMQLLEAAELIFSDEGFQVVRGYEHQFGIERRETLFHTLFIGLPSLPEDVWGAT
                     QEQTTITLH"
     gene            2778..3344
                     /locus_tag="RHW33_004633"
     CDS             2778..3344
                     /locus_tag="RHW33_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HDV1648053.1"
                     /translation="MKLQYRIPLAIYSVWCIIAILLCKDGLYQMNIWTLVEILGMIAL
                     PLLVRPFFILLRIIFRKNSFKTKDMSDNHTVCVFLSALADTSSKKAIKKHFKQLLEKL
                     PPLLRQKKRIYMKSHLLTEARTQKLICSLRRKGLDVTAERRESAMNSVMFRILIVSRI
                     LSQWKVPHINPRCGIVILTLKNDSGNRE"
     gene            3612..3866
                     /locus_tag="RHW33_004634"
     CDS             3612..3866
                     /locus_tag="RHW33_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HDV1648054.1"
                     /translation="MPQQHPGRLQVLVVDTHCKRKLFSTKTQTDPDELARRFCTPDNC
                     LVVVLCNNRFLFRLERAPGSHCRWRKGSRSRHQHLQDWLS"
     gene            3909..>4220
                     /locus_tag="RHW33_004635"
     CDS             3909..>4220
                     /locus_tag="RHW33_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pili assembly chaperone"
                     /protein_id="HDV1648055.1"
                     /translation="MSFLFKLTIAILIVFAILFPILISSDNKLSALFWKIPKDFWVFL
                     IIMYGAIITVVFIESSEQARIRRQQYLQQYKMPNEIHENVVIENSIISRETTDDESDI
                     NT"
BASE COUNT         1161 a         1020 c          934 g         1105 t
ORIGIN      
        1 tcattcgatc gtaaatagaa tgattcttag atgataaaac catttgttac acaacccgcc
       61 aattgtgtaa caacagcctc tacaagagca tatttgttca tatgttcgtc agaactgcgg
      121 attgtctgga cgagggtcag acaccgtttc gtgcatatgg accagcatgt tcgaacgcca
      181 caaagcagaa gcatcatgcc tggatcgctt cggcgcggag cgttaagttt tgacgctttt
      241 ttgtatgttt tttaagctgt tagttgactc ggtgcgttat actaagtacc ctttactggt
      301 cacaggcaaa actgtgatcg ggcgtaggaa cccgcgtagt catcgtcggc gcagaagtta
      361 atttcttcat ttggcgtcgt tttttgccat accataaaga caggaacttg tatttcttgt
      421 cgtcatctat ggtagctcag gcggggcagg cattagcctg gccggtttcc gatgacgccg
      481 gtattcctac ccccgtctgg gctatcacca atgagcgtag gaactcggtg atagcgtttt
      541 tagttgtcat cggagacctt tgctatgtca cctcaactga tacagaaact cccggccatc
      601 actctgttgg aaggcatgtt tcccgagcta tcaacaaacc agcttaaagt ctgcgttttt
      661 tacgcaatgg gtgttcctta cgacgccatt gctcagaact gtcggctttc tcccgaaacg
      721 gtacgaacct acctgaagcg aagcctgaag aatttaaatc tggaaggata tgatgcactt
      781 cgctctgctg tgctgatgcg aactttcgtt ttcatgatta gcaatacagc taaagaaaac
      841 gaaaaaatgt gaacaggtgt ccccattaaa ggggacactt tcatggaaat tgcattttgc
      901 actttacagg cgatcccaaa ttgataaaca taaacaccaa gcacaagtta ctttaataaa
      961 gcaaatcact tcagacggtg tcccagttat ttgtatctgg ctcatgcccg atatatggcg
     1021 accttactct gaactgacgt gctgatgttt tatttcgcgt atcacaatac tgtgccactg
     1081 tattgctgat attagtgctt agccgttctt tatctgtgag ttcgccaaat caccgataca
     1141 gaacgtacta gagtctgctg gttatatgcc tgtcagaact gcagtttatc cagatatggg
     1201 ctggacactg cttcagacat atgatttagc atattcgcct gacatcgcaa aatcgtccat
     1261 catggatcgg ttttgcgcgg agcgttgaac ttcgtgaata tagaacacct gaataaccgg
     1321 aactggtatc ttgcgcaata taatacagcc ggcaaaaaca gagaatcttt attctcatgg
     1381 ctgaatgagc aaaacgtcgt accatggact ccattaataa caagaaagat aagacgggca
     1441 gactccaggt gctgttaccg tgaaagaata ttcgctattt ttccagggta cttttttatt
     1501 ctggctaatt ttgacattca cccagtatca gcattacgtc gacatagcgc cttcattgat
     1561 ttcgtgaagt ttggcggtga aataaaaccc gtcaataaag atattgttga tggattaatg
     1621 aagatttacc cggaccctgt gctaaatccg ggagccaggg aagagctgaa tgcggcttcc
     1681 agcatatggc tgacaaaagc acaatatcag tatctcctca gaatggaaaa tactctccag
     1741 cctgagtcga ggatatccct gctcctggaa ctggtatcaa atgccgaaca tcacggcttt
     1801 atggagcgcc ttgtaaacat cccatagcca gtatttcccg gccttcgggc cgggcttttt
     1861 tcaattcaag agaatattcc ggagtgaagc ctgcacgaag ccatccccac catggactcc
     1921 ccctgtcttt tgatggaacc gtgtatttct taatggcgaa agaaataaaa ggcttcaccc
     1981 cagagtattg attcgtttta tcaaaggccg gcccgtgcca tcacgtgtgg cggagagaac
     2041 ggataccggt cttcttttca tctcacagta ggacattcgc atgaccacga cagaacatcc
     2101 cgtgcgccag ttgctctggt gtgcactgaa tgccctgaaa acggcacagg aaaaccacaa
     2161 tataaccagt gaaaccggat tacggcatta cctgcttgaa tggctttctg gtgccagcag
     2221 acatcctgag ttccgtagtc tccctggcga aatcatggcg ttaaaagcat tgaccgaaaa
     2281 agatcgcaat attcccatca tcggaacgct gaatacgctc tttctcagct ctgccactgt
     2341 cggggaatgt ccgttgtttc gttttcgggc tgcactgggt aaattacaga aagcaggctg
     2401 gcgcacatat gtctgcccat ggcctgagag ggtgttcaat gaatccattg aacgggcctg
     2461 ttccgggaaa cgtcatctgc tacaactaag tcgcaccgaa gagtgttttc taccgacagg
     2521 cgatatgagg gcaccagtta cgttgcagct gatcactcca ctacaccgca aaactatgca
     2581 gcttctggaa gcggcagagt taatattctc cgacgaagga tttcaggtgg tgagaggata
     2641 cgaacatcaa ttcgggatcg aacgcagaga aaccctcttt cacaccttat ttatcgggct
     2701 gcccagcctg ccagaagatg tctggggtgc aacacaggag cagacgacta tcacattaca
     2761 ctgaggaaga gcaatacatg aaactccaat atcgtattcc attagctata tatagcgtct
     2821 ggtgcattat agcgattttg ctctgcaagg atggcttgta ccagatgaac atatggacgc
     2881 tagtagagat attggggatg atagctctcc cgctgttagt ccgtccgttt ttcattctgc
     2941 ttagaataat atttaggaaa aatagtttta aaaccaaaga catgtctgac aatcacacag
     3001 tatgtgtttt cctgagcgca ctcgcagata cttcctcaaa gaaggcgata aaaaaacact
     3061 tcaagcaatt actcgaaaag ctgcccccct tattacgcca gaagaaacgt atctacatga
     3121 aatcccatct tctgactgaa gccaggacac aaaagctgat ttgctctctg cgccgaaaag
     3181 ggctggatgt aacagcggaa cgaagggaat ccgccatgaa tagcgtcatg ttccgcatcc
     3241 tcatcgtcag cagaatctta tctcagtgga aagtgcctca cataaatccc cgctgtggta
     3301 ttgtcattct gaccctgaaa aacgattctg gaaacaggga ataacagtta taagagcacc
     3361 acgatttccg acaggaaaac atacatgaaa atcaacgtag atgaccctgg caaaaagagc
     3421 ttcgctctca ttacgcctca cgtgcgttaa ttcccgacac accccgtgac ccgtcttcgc
     3481 agcctgtacc accctttctg ccgttcccgg gattctttct gtcactactc gcttgtgccg
     3541 ttttttcgca accggtacgt gcagttacga cggtacagct gcttcttttc ccgttccgaa
     3601 aggagtcttt gatgccacag caacatcccg gacgactaca ggtactggtc gtcgacacgc
     3661 actgcaaacg caagctgttt tcaacgaaaa cacagacaga tcccgatgag cttgcccgac
     3721 gcttctgcac gccggataac tgccttgtgg tggtactatg caacaaccga tttctgttcc
     3781 gcctggaacg tgcacccggt agccactgcc gctggcgcaa gggtagccgc tcccgccatc
     3841 agcacctgca ggactggctg tcgtaaaaac aacaaaaaca aaagacctcg tcaaaacagg
     3901 aggggaccat gtcgttttta tttaaattaa ccatcgcgat tttgattgtc tttgcaatcc
     3961 tttttccgat tttaatttca tcagataaca aactgtccgc acttttttgg aagataccaa
     4021 aagatttctg ggttttcctg attatcatgt acggggcaat catcacggtg gtgttcatag
     4081 aaagcagtga acaggcacgc atccgccgcc aacaatattt gcaacagtac aaaatgccaa
     4141 atgagataca tgaaaatgtg gtgatagaaa acagtattat ttcaagagaa acaacagatg
     4201 acgaatctga cattaacacc
//
LOCUS       DAPGRN010000219         4217 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPGRN010000219 DAPGRN010000000
VERSION     DAPGRN010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.223"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RHW33_004636"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RHW33_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV1648056.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="RHW33_004637"
     tRNA            complement(324..399)
                     /locus_tag="RHW33_004637"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="RHW33_004638"
     tRNA            complement(406..480)
                     /locus_tag="RHW33_004638"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="RHW33_004639"
     tRNA            complement(597..681)
                     /locus_tag="RHW33_004639"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="RHW33_004640"
     tRNA            complement(690..765)
                     /locus_tag="RHW33_004640"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="RHW33_004641"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="RHW33_004641"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV1648057.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVLPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RHW33_004642"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RHW33_004642"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV1648058.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RHW33_004643"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RHW33_004643"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV1648059.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLEPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1048 c          946 g         1144 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcactat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgctcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaaggattaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccga gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagctatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgcga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccgtcctgct caagcaataa agcgccttgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 ataggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcctaacgt ttcacccaac
     3001 tgctcacctg agtgaaattc gccattcgct aacagcgcga ttaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatcg ataagccaac
     3301 ctgctgccag ttttactgaa ccatccgcct gggggtaatt tggtgctgtt ggaaattgtg
     3361 acagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggctcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcgg tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgccct tctgcggttg catgctgcca ggcattgagt agttgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAPGRN010000220         4132 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPGRN010000220 DAPGRN010000000
VERSION     DAPGRN010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.224"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..478
                     /locus_tag="RHW33_004644"
     CDS             <1..478
                     /locus_tag="RHW33_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV1648060.1"
                     /translation="KLNAQMNQEFYTSNLYLQLSQRCSENSLNGTALFLRHQAQNTVT
                     QMMRMYEYIKQSGATPVLQEQHARCGEFSTLEDLFEQTVSDYQKRINALTSLTEDAQA
                     ANDKSAINFLKRYRKEEIVDGTLLQIILDEVRSAKKAGINMQQTDHYLVGVIDRYH"
     gene            complement(795..1775)
                     /locus_tag="RHW33_004645"
     CDS             complement(795..1775)
                     /locus_tag="RHW33_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HDV1648061.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNNEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGRVKTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            complement(2255..2536)
                     /locus_tag="RHW33_004646"
     CDS             complement(2255..2536)
                     /locus_tag="RHW33_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1648062.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
     gene            complement(2517..2846)
                     /locus_tag="RHW33_004647"
     CDS             complement(2517..2846)
                     /locus_tag="RHW33_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1648063.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            3206..>4132
                     /locus_tag="RHW33_004648"
     CDS             3206..>4132
                     /locus_tag="RHW33_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HDV1648064.1"
                     /translation="MSRRQILSLSERESLLALPDDELTLTRMAYFSEHDLALISAHRK
                     PASRFGFAVLLCYLKNVGFAPDKKIPPSDALLKHIAGRLKLTGDLWPAYLSGRETTRR
                     EHLTELYRYLGVKSFTGKIQQDCITHLLPMATRTDKGILLAEELLVWLRKNNVIIPAI
                     DVVERTCAEAMAGGDKIVFQTLNAPLTPTHKAALDRLLESSDNQSSRLTWLLQPPGKI
                     NGKNVLQHLDRLSSIELLALPEGIDRTIHQNRLLKLAREGRKMSSRDLTRFSAARRHA
                     ILVCVLEEARATLTDEVIELHERMLNSLFSKAK"
BASE COUNT          980 a         1021 c         1029 g         1102 t
ORIGIN      
        1 aaaactgaat gcgcaaatga atcaggagtt ttatacctca aatttgtatc ttcagctcag
       61 ccagaggtgc agcgagaata gccttaatgg caccgcacta tttttgcgtc atcaggcgca
      121 aaatacggtg acgcaaatga tgcgcatgta cgaatacatc aaacagagcg gcgccacgcc
      181 ggtgttacag gagcaacacg cccggtgcgg tgaattttcc acgctggaag atctgtttga
      241 gcagacggta agcgattacc agaagcgcat taatgcgctg acctctttga cggaagacgc
      301 ccaggcagca aacgataagt cagcgattaa cttcctgaag cgatacagaa aagaggaaat
      361 cgtggacggg acgctgttgc aaatcattct ggatgaagtc cgcagcgcta aaaaagcggg
      421 catcaacatg cagcaaaccg accactatct ggttggcgtg atcgatcgct atcactaaat
      481 caatttcgca gtacgaggag gttttacgcc tcctcagaaa atctgtttta catcagaaac
      541 agaggtttat gccagtgaac atttgatacc caacacgtca tagtgatttt ttaacttagc
      601 gtttttatat cgtgccaaaa atgtctctgc ttgtgtcttt atattaagga aggtgcgaat
      661 aagcaggtca tttcttccca agctgactcg ctgattaaaa tttcgcggat ctgggccgat
      721 ttttttcccg caaacacatc gaatcagcct atttaggcta ttttttccac catttctggc
      781 gttatttccg gtttttactg agatctctcc cactgacgta tcatttggtc cacccgaaac
      841 aggttggcca gggtgaataa catcgccagt tggttatcgt ttttcagcag ccccttgtat
      901 ctggctttca cgaagccgaa ctgccgcttg atgatgcgaa acgggtgctc caccctggca
      961 cggatgctgg ctttcatgta ttcgatgttg atggccgttt tgttcttgcg cggatgctgc
     1021 ttcaaggttt ttaccctgcc gggacgctcg gcgatcagcc agtccacatc cacctcggcc
     1081 agctcctcgc gctgtggcgc tccttggtag ccggcatcgg ctgagacaaa ttgctcctct
     1141 ccatgaagca gattacccag ctgattgagg tcatgctcgt tggccgcggt ggtgaccagg
     1201 ctgtgggtca ggccactctt ggcatcgaca ccaatgtggg ccttcatgcc aaagtgccac
     1261 tgattgcctt tcttggtctg atgcatctcc ggatcgcgtt gctgctcatt gttcttggta
     1321 gagctgggtg cctcaatgat ggtggcatcc accaaagtgc cttgggtcat catgacgcct
     1381 gcttcggcca gccagcgatt gatggtcttg aacaattgac gggccagttg atgctgctcg
     1441 agcaggtggc ggaaattcat gatggtggtg cgatccggca gggcgctatc cagggataat
     1501 cgggcaaaca ggcgcatgga ggcgatttcg tacagggcat cttccatggc accgtcgctc
     1561 aggttgtacc aatgctgcat gcagtgaata cgcagcatgg tctccagcgg atagggccgt
     1621 cggccattgc ccgccttggg ataaaacggc tcgatgacag cggtcatatt ctgccatggc
     1681 agaatctgct ccatgcggga gaggaaaatc tcttttcggg tctgacggcg cttagtgctg
     1741 aattcactat cggcgaaggt gagttgatgg ctcatgatgt ccctctggga tgcgctccgg
     1801 atgaatatga tgatctcata tcaggaactt gttcgcacct tccctaactt tgcaacagtg
     1861 cctgtaatat ctggcgtatt tatactgccg cagccttgga cagttcgtgg tttacgtaac
     1921 cggtattgtt cccacgtttc gagctttcca gaacgacctt caaccctcac catcggccct
     1981 gccaatgaca agtttgccca caaggcagtc tcggagattc ttttcgaccc atcagcaact
     2041 tatgcctaag tcaccggcgg cttgcctgcc acacattgaa cattacagac caattagcac
     2101 tttgtcaccg gggaaagatg taaagattat gtataaatag attggcttag aagtgacatt
     2161 tcaactttgg agaaacaggt gacattttaa acttgcttta acaaaatttt gggactgacc
     2221 gaaacaaaaa ggccagcccc ggagggctgg ctattcagta cagggcaatg acaggtttct
     2281 tgatgccgca ggcgtgagcg tagcgggtca gtgtgtcgat gctggctttg gtgatgttgt
     2341 tttccaggcg agaaataacc ggaggtgtaa cccccatgcg ttcagcaacc tgcgcttttg
     2401 tcaggttgtc atgcttgcgc cattcagcaa gcagctccct gagcttttcc ttacgttctt
     2461 cttcctgcca tgcagcgcga tattcatcgt cctgcatcca ctcgttatgc agttctttat
     2521 ggcttaccag tttcattaag tacctccgct aaccgctttt tggcgagatc gatttctttc
     2581 gctggcgttt tctgtgactt cttgatgaaa acacggagca tgatgatggt attgcctttg
     2641 tggtataccc agattcccct ggctatgtcc gtccccatcg tgcggatctc aaaaagtcca
     2701 tcgcccaggg gcttcgtgtc cggctcccgc agtaatcggg cgtcatactc cagtttgccg
     2761 atgagacggt caaacttcac cctgattttt acgggtaatg ctgtggcttc ttccctggct
     2821 tccgggtgat aaacaacgct aaacatgtga cctcccattt ctattaatat taacctatag
     2881 gctaatttca gacaaggtag aattaaccta taagctaatt ttgttggcag ttatcgtggc
     2941 tacccggtca ctaccctgtt aggggtcagt ttggagatcg gaaatttttg tacgttaagc
     3001 gcattattta accaatcttg gttcgtaagg ggttgatttt gcgtcagtgg ctatcgcttt
     3061 taagttgtcg ctcttgcagt atcgcattta ggttatccga ttaaggtcaa acctctgaaa
     3121 atgccgtata gcgcgggagt acacccttat gcgataagca tttttgttaa aaataccgca
     3181 ccgaataatt tatgagggaa aatatatgtc aaggaggcag attctttctc tgtcggaaag
     3241 agaatcatta ctggcattac cggacgatga gttaacgctg acacgaatgg cgtattttag
     3301 tgagcatgat ctggcgctta tcagtgctca ccgtaaaccc gccagccgtt ttggttttgc
     3361 cgtccttctt tgctatctga aaaatgtcgg ctttgcaccg gataaaaaaa tcccaccctc
     3421 tgatgcgctg ctgaagcata tagccggcag gctaaaactt accggtgatc tctggcctgc
     3481 ttacctttcc ggcagggaaa ccacgcgacg cgaacattta accgaactgt accgctatct
     3541 tggtgtaaaa tcgttcaccg gcaaaataca gcaggactgt attacacacc tgctgccgat
     3601 ggcgacgcgc accgataaag gtattcttct ggccgaagaa ctgctggtct ggctccggaa
     3661 gaacaacgtg ataatccccg ctattgatgt cgtggagcgt acctgtgcgg aggccatggc
     3721 tggcggcgat aaaatcgtat ttcagactct gaatgccccg ttaactccga ctcacaaggc
     3781 tgccctggat cgtttacttg agtcctcaga taatcagtct tccaggctaa catggcttct
     3841 gcaaccgccg ggtaaaatca atggtaagaa cgtgctgcaa caccttgatc ggctcagtag
     3901 cattgaattg ctggcattac ctgaaggtat agatcgtacc attcaccaga accggttgct
     3961 gaaactggcg cgggaaggcc gaaagatgag cagccgggat ttgacccgat tttcagcagc
     4021 ccggcgtcat gcgatcctgg tgtgcgtgct ggaagaagcc agagccacac tgacagatga
     4081 agtgattgag ctgcatgaac gcatgctgaa cagcctgttc agcaaagcca aa
//
LOCUS       DAPGRN010000221         4108 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPGRN010000221 DAPGRN010000000
VERSION     DAPGRN010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.225"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            112..828
                     /gene="nanC"
                     /locus_tag="RHW33_004649"
     CDS             112..828
                     /gene="nanC"
                     /locus_tag="RHW33_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV1648065.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            848..1954
                     /gene="nanM"
                     /locus_tag="RHW33_004650"
     CDS             848..1954
                     /gene="nanM"
                     /locus_tag="RHW33_004650"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV1648066.1"
                     /translation="MNKTITALAIMMASFATNASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            2018..2998
                     /locus_tag="RHW33_004651"
     CDS             2018..2998
                     /locus_tag="RHW33_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV1648067.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYSSHPQHFNHMIEAFRRDLKQYHSQLNKIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            3121..3816
                     /locus_tag="RHW33_004652"
                     /pseudo
     CDS             3121..3816
                     /locus_tag="RHW33_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1166 a          932 c          959 g         1051 t
ORIGIN      
        1 ccggttgcca aagacactat aagcctggca aaaaaatatt acacaacata aatgctaatt
       61 gtttatgcgg gctttgtatt gctttctgta tcctacaaat gagtgaaatt tatgaaaaag
      121 gctaaaatac tttctggcgt attattactg tgcttttcgt ccccattaat ttctcaggct
      181 gcgacactgg acgtacgtgg tggatatcgt agtggaagcc acgcctatga gactcgactc
      241 aaagtcagtg agggatggca aaatggatgg tgggcaagca tggaaagtaa tacctggaat
      301 accattcatg ataataaaaa ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat
      361 tacgcgatta aacttgatga tcaatggacg gtgcgcccgg gaatgttaac gcattttagc
      421 agcaacggca cacgctacgg accctacgta aaactgtcct gggacgcgac aaaagatctt
      481 aattttggca ttcgctatcg ttacgactgg aaagcttacc gacaacaaga cttatccggt
      541 gatatgtctc gtgataacgt tcatcgttgg gatggatatg tcacttacca tattaatagt
      601 gatttcacct tcgcatggca aacgacgcta tacagcaaac agaacgatta tcgctatgca
      661 aaccataaga aatgggcgac ggaaaatgca tttgttctac aataccatat gacgcccgat
      721 attacgccat acatagaata tgactacctt gaccgtcagg gtgtttacaa cggcagagat
      781 aatttatcgg aaaacagtta tcgcattggt gtgtcattta aactgtagta gacaggagac
      841 agtcacaatg aataaaacaa taacggcgct tgctatcatg atggcttcat ttgccacaaa
      901 cgcgtctgta ttaccggaaa ctcctgtgcc atttaaaagt ggtaccggag caattgataa
      961 cgacactgtc tacattggtt taggtagcgc aggtacggca tggtacaagc tggatacaca
     1021 ggccaaagat aaaaaatgga cagcgttagc tgcattccct ggcggaccaa gagatcaagc
     1081 aacctctgca tttattgatg gcaatctgta tgtgtttggc ggcattggca aaaatagcaa
     1141 gggcttgact caggtattta atgacgtaca caaatacaac cccaaaacca atagttgggt
     1201 taaattgatg tcgcatgcac cgatgggcat ggcgggtcat gtgacttttg tacacaacgg
     1261 caaggcttat gttactggcg gtgttaacca gaatatcttc aatggctatt ttgaagatct
     1321 caacgaggct ggaaaagatt cagcaactat agacaagatc aatgcccatt attttgacaa
     1381 aaaagcagaa gattatttct tcaataagtt tctgttgtct tttgatccct caacacagca
     1441 atggagttac gctggcgaat ctccctggta cggaacggct ggtgcggcgg ttgtgaataa
     1501 aggtgataaa acctggctta ttaatggcga agccaaacca ggattgcgaa cggatgccgt
     1561 atttgaactt gatttcaccg gtaataattt aaaatggaat aagcttgctc ccgtctcatc
     1621 accagatggc gtcgctggcg gttttgcggg gataagcaat gattctctta tatttgccgg
     1681 aggggccgga ttcaaaggtt cacgagaaaa ttaccagaac ggtaagaact atgcgcatga
     1741 aggcctgaaa aaatcatata gcactgatat tcatctttgg cataacggga aatgggataa
     1801 atcgggtgaa ttatcgcaag gtcgggccta cggagtatca ttgccctgga ataatagtct
     1861 attgattatt ggcggtgaat ctgcaggcgg caaagcggtg acggattcag ttttgatctc
     1921 tgtgaaggat aataaagtta cagtacaaaa ctaacgcttc agggccccag taaagggccc
     1981 tgctatcaat ttatcatttc aattacgcga aaattatatg gacgcaataa tatcgcccga
     2041 ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg gcgaaggact
     2101 gccattaccg gacagggaag atgcgcctca tcccagaatt aaacaattag cgagatttgc
     2161 gcatacgcat cccggaggcc cgtcatgtca ctttaacgac atgattccac tgactcactg
     2221 cccacacgat gttcaggata tgcagggtta tcatcatccg ctggcaacga atcatcaaac
     2281 acagtacggc accgttggcc aggcactgca tattgcacgg aaattactgc cctttattcc
     2341 tgataacgca ggggttctca tcgttccatg ttgccgtggc ggatcggctt ttaccgcggg
     2401 cagcgaaggg acatattcag aacggcacgg agccagtcat gatgcttgtc gttggggaac
     2461 agatactccg ctataccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc
     2521 gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga
     2581 ctactcgtca caccctcaac actttaatca tatgattgaa gcctttcgta gggatctaaa
     2641 acaataccat tcgcagctta ataaaattac tgacgcgccg tggttttgcg gcgataccac
     2701 ctggtactgg aaagaaaatt tccctcatgc gtatgaagct atttatggca attatcaaaa
     2761 taatgtttta gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac
     2821 gaacgcgcct gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg
     2881 gtcaccggag aactggacga cggcactgcg aagcagtcat ttcagcgcgg cagcccgtcg
     2941 ggggattatt tctgacagat ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc
     3001 gttcattaat ttgatttatt cgcatccatc agcgctttta actcctccgg cgtgcggcac
     3061 tataacgact tccacaacaa ggagccattt tcttatacca ctttcagtga ccatccgctt
     3121 atgccaccac cctctacgca actgtttgca gagcaccttc ccaccgagtg gatacagcac
     3181 ttcctgacgc tttctgctca tgcgaccgtt cgtcgtctta acctgagcgt ggatggcgaa
     3241 gcggggatga acctgctggc gcccgcagcg ctgtcaccca ggcgcgtcag cgcgtggggg
     3301 ccgccccggt ggaatggctc ttccgccaga ccacacagac atggggcgcg gaacgttacc
     3361 tgaaggatga ctggcacggc ctgcaacttt ttgccattga tagcgcacag ttcaggacac
     3421 ctgatgaacc cgagctgcgt gaatattatg gctctgccaa cacatccact gagcggcaga
     3481 gcgcctaccc cgtcatgcgt ctggtggcgt taatgaatct gggaatcaca ttctactgaa
     3541 tgccatgacc gcgcctaccg gcggagcgat acggcgtgag gcaacaaaag cggcgtcaga
     3601 gatcagcttt aagttcgcgt tccagtttat cgtgacagaa atgatagtgc tgggaaacac
     3661 ggcgtcaccg gggactatcc cgaaacgact ggagcatctg cgggggctct ggaagtggtg
     3721 ttcataacaa aacgcccccg accatcaagg gcgagggcgg ttaagatatc aaaaacccgt
     3781 tatcctgtga aacacagtgc cgccccgctt aagtgaacgg cattgtgttt ctgagggacc
     3841 ttaattttcc ctgattctct gacgttcctt accattgata tttccttcat tcctcaccag
     3901 agaaataaaa aatcactata ctcatcaaaa tatgagatta cctttattca ggtgatcacc
     3961 ttactatttt ttacggaaaa taaattatga ataacaagaa atcactgaca aaccacctgt
     4021 aaaatgtcaa tcattttagt tcaattgcct ttgtcatgat tatcttttta tcgtcctgac
     4081 gatactatta acaattgcaa tttttata
//
LOCUS       DAPGRN010000222         4061 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPGRN010000222 DAPGRN010000000
VERSION     DAPGRN010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.226"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(311..1168)
                     /gene="sitD"
                     /locus_tag="RHW33_004653"
     CDS             complement(311..1168)
                     /gene="sitD"
                     /locus_tag="RHW33_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HDV1648068.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(1165..2022)
                     /gene="sitC"
                     /locus_tag="RHW33_004654"
     CDS             complement(1165..2022)
                     /gene="sitC"
                     /locus_tag="RHW33_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HDV1648069.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2019..2846)
                     /gene="sitB"
                     /locus_tag="RHW33_004655"
     CDS             complement(2019..2846)
                     /gene="sitB"
                     /locus_tag="RHW33_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HDV1648070.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(2846..3760)
                     /gene="sitA"
                     /locus_tag="RHW33_004656"
     CDS             complement(2846..3760)
                     /gene="sitA"
                     /locus_tag="RHW33_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HDV1648071.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPINAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1115 a         1027 c          954 g          965 t
ORIGIN      
        1 ggcattatac gtcttcgtta ccggaagata ttcttaaggg tgagttaaga tcattaaact
       61 tcaatacaaa caattaatta aactcttagc gtggtttttt acatcactgg ccctcgcccc
      121 ccaattttct ctatgttttg ccactacttc cctcatttta ctgcgctgtt tgctatccct
      181 gagaatcgac attacttgct atccagtccg cagcaactct ctggcaacaa aacttatcag
      241 aatcataagt ctgtgcatta ttcgttcaag cgcatcgcca ataagcggat gttgctggcc
      301 gagtgttctg ctaatcggat gataacaaat cccgtggttc ggcattttcc tttgtgcgtg
      361 cgttgatgct ggtaacggta aacgtcatga tgaacatgac cgcgaataag acaacaattg
      421 tcggagctgg cgcgctgtcg ataaaaaagg acagccagac accagtcatt gagaccaata
      481 tcgatatgcc agtcgccagc aggagcgcaa tatgaaagcg ttgtgtcagg agtaccgcaa
      541 tggcaccagg cgcaatgaga agagaaatag agagaatgat acctaccgct ttcagcgtcg
      601 ccacaatggt cagggagacc atgcacagca gcccataatg cagccatctc gtatgcaaac
      661 cgcttgcctg cgcctgttga taatcaaagc tgaacagcaa aaaatcccgc cattttacgc
      721 taataacaag tgtaaccagt ccggcaataa tcaccgtctg gattatatcg ccgatggtta
      781 tcccgagcat gtcgccgaaa agaatatgat caagatgtac gtctggtttg actgcaatat
      841 acaagatcag acctgcggca aacatgccag aaaaaacaat ccccatcact gtatcttgct
      901 tgattcggct gttgtctttc aggtatcccg tcgccaccgc acagaatact ccggcgacga
      961 atgcccctgt agccagaggt aaccccagaa tccaggcaag cacgatgcca ggaaaaaccg
     1021 catgactcat cgcgtcaccc atcagtgccc aacttttcag gaccagaaag accgacagca
     1081 gcgcacaggg tatggaaact accagtgaaa tcagcagtgc gtgactcata aaggtgaact
     1141 gcagtggctc cagtaataat gccatcatga gcgatcctcc agcgctttat gcgcacgccg
     1201 acgatttgcc aaaagcccat gggtgggcgc aaagacaaat gctaaaagaa acagcagtgt
     1261 ctgcgccacc acgatgattc cacctgtcgc gccgtccaga aagtaactga cccacgcgcc
     1321 gaggaaactg gtcacactgc caatagttac agcaatcatg agcaggcggg gaaaacgatc
     1381 ggttaacaac catgctgttg caccgggagt caccaccaga cagatcacca gaaaggcacc
     1441 aaccgtttgc agcgccgcaa cggtcgaaac cgacagcagg gtgaagaaga ggatttttaa
     1501 tctgcccgga tgcaatccaa tagcacgggc gtgattctcg tcgaaaaagg tcaccataag
     1561 atctttccat ttgaaaaaaa gcacgatgat cgagagaatg ccaatgatag tgagttgcag
     1621 gatatccgcc ggatcgattg ccagaatatt gcccaggacg atggtctgaa tgttcacgga
     1681 tgtcgggttc aatgacacca taaacagccc caggccaaaa aaggacgaga agatcaggcc
     1741 gatgatggca tcttctttta agcgagtgag ttggttaaga aacagcatgc tgcccgctgc
     1801 cagtccgccg gaaaagaagg ctcccagaga aaacggaagc cccagcatat atgcccccgc
     1861 aacacccggg acaatagagt gcgacagcgc atcgccaatc agcgaccagc cttttagcat
     1921 cagatagcat gacaggaaag cgcagagacc gccgaccatc gccgagaccc acatcgcatt
     1981 gagcatatat tcatagctga agggttccag tagcacgttc atctttcttc cctctgcacc
     2041 gctgacggtc gatgtgccac aaaagggcgt tcatcgtcag taataatgct ttcttccgag
     2101 ccgttaagtg tgacatggcg cagtacgccg ctaaaagcca gctccaggtt ctcggctgta
     2161 aaagtggtgt ctgtcggccc gcttgccagg acggtgcctt tgaccatgac cgtataatcg
     2221 caaaatgtcg tgacggatcc gagattgtgc gttgagacaa gcatcgtttt gccttccgcg
     2281 cgtaactccc tcaacaggct gatgattttc gcttcggttt tcacatcaac gccggtaaaa
     2341 ggttcatcca gcaggatcac atcaccctgc tgtgcaatcg ctctcgccag aaagacgcgt
     2401 ttcttttgtc caccggaaag ctcgccgatt tgtcgatggc gaaaatccac catatcgaca
     2461 cgttccagcg catcggtgac aatctgacga tccctctttt tggcgatacg caacataccc
     2521 atatgcccat agcggcccat catcaccaca tcttccacca gtacaggaaa tgaccagtca
     2581 acctcttctg actgagggac gtaggcaacc aggtttttct gtagcgcctg tcgtgtggga
     2641 atacccagaa cagatatctt cccgctggtc agacgcacaa atcccataat cgctttaaac
     2701 aacgtcgatt ttccggaacc gtttacccca accagagcgg caatagatcc gcctggtact
     2761 gtgaaggatg catcgcgcag tgctgtgtgc ccgttacgcc aggtgacagt aacatcatta
     2821 acgacaatgc ctgcagattg catcattatt tctccctttt tccggcttta attccctgta
     2881 ccagcgtact ggtagtaacc ttcagaaggt cgatatacgt aggtaccggg ccgttttctg
     2941 tgctcaggga atcgacatag agcacaccac cgtagtgcgc gccggtttca cgcgcaacct
     3001 gacgcgctgg tttatcggaa atcgtactct cgctaaagac tgccgggata tgatttttct
     3061 taactatatc aacaacctta cgtacctgct gcggtgttcc ttgttgatcg gcattaatcg
     3121 gccacagata aagctctttt agccccaaat cgcgtgcgag ataagaaaaa gccccttcac
     3181 tggtgaccat ccatcgctga ttctcaggga gttccgcaat ctgcttacgc aggggggcaa
     3241 gggtttgggt aatcttggct ttataagtat cggcattacg ttggtaggtt tgtgcattta
     3301 tcggatcgta ttttataaac gcatcacgaa tattatcgac gtaaatcaga gcattatctg
     3361 gcgacatcca ggcatggggg ttaggtttgc cctcataggg cccttcggtg atccctactg
     3421 gcgtcacacc cgaagagaca attacttctg gaaccccatt gagatgctgg taaaagcgtt
     3481 ggaaccacaa ttccagattc ataccattgg cgagaatcag ttgtgccccc tgcgcacgtt
     3541 taatatcgcc aggggtaggc tgatactcat gaatttctgc accaggcttg gttatggatg
     3601 agacttctgc agcatctcca gccacgtttt tggccatatc tgcgatgatg gtaaaagttg
     3661 taatgacctt gaatttttca gctgcactcg cctgaaatgc tatcgagcag gtgaggacta
     3721 ggcaccccaa gagcatggtt acttttttta ttgagagcat gatttagtac ctatagtgag
     3781 caaaattaga ataaacatag caactactat gttttatagc agaagctata aaatgtaaat
     3841 aattatcatt agcgattata agatcccaac acagggatta ggttatttcc tgctgacgat
     3901 gtggtattga ggataaaagt tgggcagcgc tttggcttta aaatgaactt gttcaggacg
     3961 attggtaatg gcaaagcaaa aataatgtag gttattggca agttctctag gacgcaggag
     4021 agcatgaaaa acccaggggc agtcactaaa tgaagggatc g
//
LOCUS       DAPGRN010000223         3868 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPGRN010000223 DAPGRN010000000
VERSION     DAPGRN010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.227"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(294..1451)
                     /gene="intS"
                     /locus_tag="RHW33_004657"
     CDS             complement(294..1451)
                     /gene="intS"
                     /locus_tag="RHW33_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDV1648072.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSIGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NENEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWIDEKVE"
     gene            complement(1613..1687)
                     /locus_tag="RHW33_004658"
     tRNA            complement(1613..1687)
                     /locus_tag="RHW33_004658"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1652..1654),aa:Arg,seq:cct)
     gene            complement(1763..2695)
                     /locus_tag="RHW33_004659"
     CDS             complement(1763..2695)
                     /locus_tag="RHW33_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV1648073.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2988..3743
                     /gene="mlaA"
                     /locus_tag="RHW33_004660"
     CDS             2988..3743
                     /gene="mlaA"
                     /locus_tag="RHW33_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV1648074.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          943 a          918 c          920 g         1087 t
ORIGIN      
        1 atggtgctag gtggcatttt ggtgttggag ctcaaactgt tggtaatata atgaggaagc
       61 acggacttga tcctagtaag tatgggtttt ggtgtcacga taaaacgcat cgcgaacaca
      121 tgacaggaaa tatagatatc ttttgtgaaa gttatggtat aagatatgac gaacttgcaa
      181 tgtttatatt ggcatcaata tctttatgtg aataaacttt gtaaatgaag tgaattgcaa
      241 atccttgtac ttgattgcaa ggctttgtgc tcttcgataa tggttaaggt ggatcactcc
      301 accttttcat caatccagtc cgcccaccac tgcatcattt ctctgcgctt atcgagatac
      361 tgagcatggt tgtaaattcc acgcacagat ccgccgttgg catgtgccag ttgcacttca
      421 atagcatcag caggccattc gttctcgttc ataatcgtgc tgaattcatg cctgaatccg
      481 tgaccgcttt ccagaccctc atagccgatt tgtttgatca caagcaatac cgcgttctcg
      541 cagattggct tcttcttatc gttgcgcccg gcaaaaacaa actctgatac tggtttagtg
      601 atggagctta gcgtagtgag aagttcaacc acctggtctg acataggaac aacatgaatc
      661 ttgcggccct tcatcacact ggcgtcgata gtgataatcc tgttttcaaa atcgacgttc
      721 ttccattgca tggaacgaag ctctttcgtt cttagggctg tgtagcgtaa aactttggtc
      781 gcaatgagcg atacgatact tcctgaaaat gttgcaagtg ctttgttgaa tgccgggatc
      841 tggtcggcag gtaaaaacgg gaagttcttc ttgcggtatc ccttcatggc gtctgcaagg
      901 tcaggtgccg ggttatattt agccctacca gtgacaatag cgtaacggaa aacctcgccg
      961 catcttctgc gggctttgtt ggctcgctcc attgcaccgc gatcttcaaa cctgcggatt
     1021 acttccaaca gttgcatcgg ctcaatatcc tgaatctcaa gaccgccgat gatgggtaaa
     1081 atgtcgtcat caaacatttt ggcaagttca gttgcatagc ctattgacca gacttgcttc
     1141 ttgtgctcgt accattcctt gtaaatcgca ctaaaggaat tgttgttaga cgaagccttt
     1201 ttcgctttta ccggatcgat gccaacagag atgtctttcc tcgcggtcca tgctttatcc
     1261 cttgcctcct gcaaagtcat aagcggatat tttccgacgg tcaggatttt ctccttaccg
     1321 tcaatcttgt agcgaagctg ccataccttt ttccctgata cagggacata aaggtacagg
     1381 ccattaccat cgagtaggcg gtatggtttt tctttcggct ttgctgcttc aatctgctta
     1441 acggtgagca tgggtaaaaa tccggtgggt aaaattattt tatccacttt ttacccgtca
     1501 tggagtgcgg ctgtcaacga tctgacgcga accattacga actgtgaatc tacggaagtc
     1561 ttggtattca ggggattttg cggactggta cggatgggag cgaactgata aatggtgtcc
     1621 cctgcaggaa tcgaacctgc aattagccct taggaggggc tcgttatatc catttaacta
     1681 agaggacaat gcggcatgag tatacccgct aatggactgc ggggtaagta cgctgccgct
     1741 cgattgctta aaccctcgcc atttatgccg ggtttttatc atttttctta atgttttccg
     1801 cacgttctgc tttttggcgt gcttctgctt tgcgcttgtt gctcatgtcg ttacggatct
     1861 gtgcatgact cattaacgcg aagataaagg tgccgccgca gatgttcccc gctaaagtag
     1921 gtagtgcgaa gggccagatg aaatcgctcc agtgcagtgt accgttaaac accagataga
     1981 ggatttcaac agaaccgaca acgatgtggg tggtgtcacc cagggcaata agccaggtca
     2041 tcaatataat caccacaatc tttgccgcac ccgctgcagg aaacatccat accatagtgg
     2101 cgatcagcca gccggaaatg atcgcgttgg caaacatctc gctgggggtg ttcttcatca
     2161 catccatgcc gattttgaca aatgcatcgc gagtttcttc attgaagata ggcatatatt
     2221 caaatgccca cgccgcaata cctgtcccga gaatattacc cagcagcacg acgccccata
     2281 accgcataag taagccaacg ttgctcattg tcggtttttg catgacgggt agtaccgcag
     2341 tcacggtgtt ttcggtaaat aattgctggc gggccataat gacgataata aaaccaaagg
     2401 tataaccgag attctccagc aagaagctgc ccggcacacc ttccagttcg acatgaaata
     2461 tcccttttgc cagtaatgaa gcgcccatcg acagacccgc cgcaatggct gaccacagta
     2521 gtgccattgc gtcgcgttcc agctcttttt caccatcctg gcggatatgc tcatgaattg
     2581 ccatcgcccg ggaggggagt cgatcttcat ctatttctat ttttttgcca cgctcttttt
     2641 cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat tttgtcgttg tccatggtct
     2701 cttctcttga attagcacgt atagctaaag cgtagcggct ttttgctcgc aactggcggg
     2761 agttactctg aaaatgtaga aaaggccgcg tttgcctttt tctgtttcca tagaatcaag
     2821 tagcctacag ggcggcgatt accaggctat gatcaaatca gcaaatcagg gcgtctggac
     2881 atcagttgac gtgctgttac aatcgcccac acctaaacag gcggatacgg tatcgttccg
     2941 tcatggatgg caaactgcat aagccataaa aaaacaggga gacatttatg aagcttcgcc
     3001 tgtcggcgct tgctctggga actacgcttc tggtggggtg tgcgagttcc ggtacagatc
     3061 agcaagggcg ttctgacccg ttagaagggt tcaaccgcac catgtacaac ttcaacttca
     3121 atgtattaga cccgtatatt gttcgaccgg tcgctgttgc ctggcgtgat tatgttccac
     3181 aaccggcgcg taacggtttg agcaacttta ccggcaacct tgaagaacct gcggtgatgg
     3241 ttaactactt cttgcagggc gacccttatc aggggatggt ccactttacc cgctttttcc
     3301 tgaacaccat tttggggatg ggtggtttta ttgatgttgc agggatggcg aacccgaaac
     3361 tgcaacggac tgaacctcac cgcttcggta gtacgcttgg tcattatggc gtgggttatg
     3421 gcccgtacgt tcaattaccg ttctacggta gcttcacgct gcgtgatgac ggtggtgata
     3481 tggcggatgg tctttacccg gttctttcct ggttgacctg gccgatgtct gtgggtaaat
     3541 ggacgcttga aggtatcgaa acccgcgcac agctgctgga ttccgacggt ctgctgcgtc
     3601 agtcgtccga tccttacatt atggtgcgcg aagcgtactt ccagcgtcat gatttcatcg
     3661 ctaatggcgg cgaactcaaa ccgcaggaaa atccgaacgc gcaagcgatt caggatgatt
     3721 taaaagatat cgattctgaa taagaaacaa ataaaaaagg tgagtcgcaa aactcacctt
     3781 ttttgttgtt atccctcaat taccacaagt aaaaggagat aaaccgacaa tgacccggac
     3841 gtaatgatgt aaaaatactg atgttcgt
//
LOCUS       DAPGRN010000224         3861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPGRN010000224 DAPGRN010000000
VERSION     DAPGRN010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.228"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..1728
                     /gene="dpiB"
                     /locus_tag="RHW33_004661"
     CDS             70..1728
                     /gene="dpiB"
                     /locus_tag="RHW33_004661"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV1648075.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1697..2377
                     /gene="dpiA"
                     /locus_tag="RHW33_004662"
                     /gene_synonym="citB"
     CDS             1697..2377
                     /gene="dpiA"
                     /locus_tag="RHW33_004662"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV1648076.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2417..3802)
                     /gene="dcuC"
                     /locus_tag="RHW33_004663"
     CDS             complement(2417..3802)
                     /gene="dcuC"
                     /locus_tag="RHW33_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV1648077.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1036 a          935 c          999 g          891 t
ORIGIN      
        1 ctatgtattt tgattcaaca ttttaattac atccgtcaaa gaggctcggg acaacccgca
       61 aggaaaacaa tgttgcagct taacgagaat aaacagtttg catttttcca aagactggca
      121 tttccgctgc gtatcttttt gctgattctg gtgttctcaa tatttgtcat tgcagccctg
      181 gcgcaatatt ttacggccag ttttgaggac tatttaacgc ttcatgtacg cgacatggca
      241 atgaatcagg cgaaaattat tgcctccaat gacagtgtca tctctgcggt gaaaacgcgt
      301 gactacaaac ggctggcgac catcgctaac aaattacaaa gagataccga ttttgattat
      361 gtggtgattg gcgaccggca ctcgatccgc ctttaccatc ctaatccgga gaaaattggt
      421 tatcctatgc agttcaccaa acagggcgcg ctggagaaag gggagagcta cttcattacc
      481 gggaaagggt caatgggtat ggcgatgcgc gccaaaacgc caatctttga tgacgatgga
      541 aaagtcatcg gcgtggtgtc gattggctac ctggtgagta aaatcgatag ctggcgggct
      601 gagtttttat taccgatggc aggtgtgttt gtcgtgctgt tagggattct gatgttgctg
      661 tcgtggttcc tggccgcgca tatccgtcgg cagatgatgg gcatggagcc aaagcaaatc
      721 gcacgcgtgg tccgtcagca agaggcgctg tttagttcgg tttatgaagg gctgattgcg
      781 gtggatccgc acggttacat taccgccatc aatcgtaacg caagaaagat gctggggctg
      841 agttcccccg gacggcaatg gttgggtaaa cccattgctg aagtggtcag gcccgccgat
      901 ttctttaccg aacagattga tgaaaaacgt caggatgtgg tggctaactt taacggtctg
      961 agcgttattg ccaatcggga agctattcgt tcaggtgatg atttgctggg ggccattatc
     1021 agctttcgta gtaaagacga aatttccacc ctcaatgcgc aactgacgca aataaaacaa
     1081 tacgtcgaga gcctccgtac attgcgacac gagcatctca attggatgtc gacgctcaat
     1141 ggtctgttgc agatgaaaga gtatgatcgc gtgctggcga tggtgcaggg ggagtctcag
     1201 gcccagcaac agcttattga cagtctgcgc gaggcgtttg ccgatcgcca ggtggcgggg
     1261 ctgctttttg gtaaagtgca gcgcgcccgc gaactggggc taaaaatgat catcgtcccc
     1321 ggtagccagc tttcgcaact gccgccagga ctggatagca ccgagtttgc cgccattgtt
     1381 ggcaatttac ttgataacgc cttcgaagcc agcctgcgta gcgatgaagg aaacaagatc
     1441 gttgaattat tcctcagcga tgaaggcgat gatgtggtga ttgaagtcgc cgatcagggc
     1501 tgcggcgttc cagagtctct acgagacaaa atatttgagc agggggtcag tacgcgtgct
     1561 gacgagcccg gtgaacatgg cattgggttg tacttgattg ccagctacgt aacgcgctgc
     1621 ggtggtgtta tcactctcga agataatgat ccctgcggta ccttattttc aatctatatt
     1681 ccgaaagtga aacctaatga cagctccatt aaccctattg atcgttgagg acgaaacgcc
     1741 gctggcagag atgcatgcgg aatatattcg tcacattccc ggattcagtc agatattact
     1801 ggcgggaaac ctggcgcagg cccgaatgat gatcgagcgt tttaagccgg ggctaatctt
     1861 gctcgataac tatcttcctg acggtagagg gattaattta ctgcatgaac tggtgcaggc
     1921 gcattatccc ggcgacgtgg tgtttaccac tgcagccagc gatatggaaa cggtgtctga
     1981 agccgtacgt tgtggtgtat ttgattatct cattaagccc attgcctatg aacggctggg
     2041 gcaaacgcta acccgtttcc gccagcgtaa acatatgctg gaaagtattg atagcgccag
     2101 ccagaagcaa attgatgaga tgtttaatgc ctatgctcgc ggtgaaccta aagacgaact
     2161 gccgaccggc attgacccct tgacgctaaa cgcggtgcga aaattgttca aagagcctgg
     2221 tgtgcaacat acggcagaaa cggtagcgca ggcactgacc atcagccgca ccactgccag
     2281 gcgttatctt gaatattgcg ccagccgcca tctgattatt gctgaaattg ttcacggcaa
     2341 agttggcaga ccgcaacgca tataccacag tgggtgacat gataaagccg gaggaaactg
     2401 cctccggcat tactatttac ttgcctgtga ccgctgctgc cgttcctggc accatcagct
     2461 ctgtagcaac gataacaatc accagcccaa caagcaccgg caccgaggtg cgttttacga
     2521 cttcaaatgg tgagattttc gccatcccgg caaccgcaac gactacgcca gaaacgggtg
     2581 acagggtacg accgaggttt gacgcctgca gcatcgggat agtcaaatac gccgggttaa
     2641 tgccggaaga gtgcgccagt ttcgggatca tctcaacaaa cgcataaaac ggcgcattgc
     2701 ctgaaccggt cgtgactgcc gccagcattg tcagaatcac caataccagc atcaggatga
     2761 tactcgccga accaaacgag gtagcgatag aaatcagact ttgaataaag ccgatggtgc
     2821 taagcccctg agcgaatacc ccagcggcaa ccagcagcat caccacgttt gcaaatgcat
     2881 ctgccatccc gcgataagcc acttccagac cagagaaaac tttctgggta ttaaagctgc
     2941 ggaggaactc cagaatggag gcaatcagca tacaaatcac cagaatagtg atgatgtgta
     3001 attgcggacc ccatttaccg tcaaaaatca gtacaccgat gatcggcgtg aacggcaaaa
     3061 tggcataaaa cgcaggagca gtggtggtga tttcactgac atctaacatt tcatgagaga
     3121 tgtgctcttt tttatccaga taacgttgcc agaagaagtg ggcgatcgcc atgccgataa
     3181 ttgcagcaat tgagataggc agcgtcgttt tgaaggcgaa gtcaatcagc gacatttcgg
     3241 aagcttgcgc cgccagcacc acatcccctg aagtcggtgc gagaataatc gccgccgggg
     3301 aggcgcaaat ggcagctgct gccccacgac tgataccaac gtttaccatc accggaaaca
     3361 gggttgccat cagcaaaaca cccagaccgg ttgcggaaga gacggccagt gacatcagac
     3421 aggcaacaaa ataggcggca atcatcagca ggtaagggga gttaatatac tgcaatggtt
     3481 ttgacgccag cttgaccacc atatcattcg cgccgatatg ggtcatgtaa gcggcaaagc
     3541 cacacagcat cataatcatc atgccgaggt cgccgccgcg gctcattagt aatattttaa
     3601 cgtattcaac gatatccgtg gcgctgtagc ctgttgaagc ctggctggac ggtaacactt
     3661 tgtgccccat aatggcactg ataatcagca ataacaggcc accgacaaat aacacaccag
     3721 tggcggaata ccctttaatg atgtagcgag ctacacccac aataaccaca accccaataa
     3781 ggagttcaat gaatgtcagc ataatttttc ctgtctccag gccccaaagt aaataataaa
     3841 aaattcctaa aacttaagga a
//
LOCUS       DAPGRN010000225         3831 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPGRN010000225 DAPGRN010000000
VERSION     DAPGRN010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.229"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            184..504
                     /locus_tag="RHW33_004664"
     CDS             184..504
                     /locus_tag="RHW33_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV1648078.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
     gene            497..883
                     /locus_tag="RHW33_004665"
     CDS             497..883
                     /locus_tag="RHW33_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDV1648079.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            891..1577
                     /locus_tag="RHW33_004666"
     CDS             891..1577
                     /locus_tag="RHW33_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV1648080.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1555..2178)
                     /gene="tetR(B)"
                     /locus_tag="RHW33_004667"
     CDS             complement(1555..2178)
                     /gene="tetR(B)"
                     /locus_tag="RHW33_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV1648081.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2260..3465
                     /gene="tet(B)"
                     /locus_tag="RHW33_004668"
     CDS             2260..3465
                     /gene="tet(B)"
                     /locus_tag="RHW33_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV1648082.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3578..>3831)
                     /locus_tag="RHW33_004669"
     CDS             complement(3578..>3831)
                     /locus_tag="RHW33_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1648083.1"
                     /translation="PIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNL
                     LNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT         1081 a          695 c          829 g         1226 t
ORIGIN      
        1 aaaagtataa ataataaaca aaattataag agaaggagaa catgataagc gcaggccgag
       61 caatttaaag aagtgtgata tacaacataa aacagcagca tgacctttct atatttcgtt
      121 gcttatcgaa gtgttaattt tcgggtggat tactctgttt gttgattaat aacggagaaa
      181 aatatgattg ctgtaatatt tgaggtgcaa atacaacccg accaacaaac tcgctatttg
      241 actttagctg aggagttaag accactatta agtcatgtag ctggttttat ttcaattgaa
      301 cgttttcaaa gtctagctac agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa
      361 tacgcagttc tgcaatggaa aaatcatgtt ttacatgcga aagctcaaca agaagggcga
      421 gagtcaatat ttgattttta caaaattagt attgctcata ttactcgcga atattcattt
      481 aaaaaggaca aggataatgt ttgatgttca cgttgtttta gataatcaaa taggacaatt
      541 agcattacta ggaaaaacat taggtaataa aggtattgga ttggaagggg gagggatatt
      601 tacggttggt gatgaatgcc atgctcattt tcttgttgaa caaggaaagg aagctaaaat
      661 agcgctagag caagctggac tgttagtact tgcgatccgg acaccattaa ttcgtaagtt
      721 aaaacaggaa aaaccggggg aacttggcga aatagcacga gtattggcgg agaataacat
      781 taatatttta gtgcaataca gtgaccatgc taaccaactg atattaataa cggacaatga
      841 tagtatggct gcatctgtta cgctcccttg ggcaataaag tgaacttgcg atggctaatt
      901 taatacgaaa agaggttacc tttgagtcct caatagccgc gataggggcg gctatgtctg
      961 acatttcacg agttaaaata ctcagtgctt tgatggatgg gcgagcttgg acggccactg
     1021 agctaagttc tgtggcgaat atatcagctt caacggcgag cagtcattta tctaaattat
     1081 tagattgcca gctaatcaca gtagtagctc aaggcaagca tcgttatttt cggctagcag
     1141 gaaaagatat tgctgaattg atggaaagta tgatggggat ctccttaaac catggcgtac
     1201 atgccaaagt ttccacgcca gtgcatttac gaaaagcacg tacttgctat gatcatttag
     1261 ctggcgaagt tgccgttaag atctatgatt ccctttgtca acagcaatgg atcactgaaa
     1321 atggttcaat gatcacatta agtggtattc aatattttca tgaaatggga attgacgttc
     1381 cttccaaaca ttcacgtaaa atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc
     1441 atttaggtgg gtacgttgga gccgcattat tttcgcttta tgaatctaaa gggtggttaa
     1501 ctcgacatct tggttaccgt gaagttacca tcacggaaaa aggttatgct gcttttaaga
     1561 cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa
     1621 gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg
     1681 catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc
     1741 caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag
     1801 tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc
     1861 tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct
     1921 aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa
     1981 gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt
     2041 ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt
     2101 tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact
     2161 tttatctaat ctagacatca ttaattccta atttttgttg acactctatc attgatagag
     2221 ttattttacc actccctatc agtgatagag aaaagtgaaa tgaatagttc gacaaagatc
     2281 gcattggtaa ttacgttatt cgatgccatg gggattggcc ttatcatgcc agtcttgcca
     2341 acgttattac gtgaatttat tgcttcggaa gatatcgcta accactttgg cgtattgctt
     2401 gcactttatg cgttaatgca ggttatcttt gctccttggc ttggaaaaat gtctgaccga
     2461 tttggtcggc gcccagtgct gttgttgtca ttaataggcg catcgctgga ttacttattg
     2521 ctggcttttt caagtgcgct ttggatgctg tatttaggcc gtttgctttc agggatcaca
     2581 ggagctactg gggctgtcgc ggcatcggtc attgccgata ccacctcagc ttctcaacgc
     2641 gtgaagtggt tcggttggtt aggggcaagt tttgggcttg gtttaatagc ggggcctatt
     2701 attggtggtt ttgcaggaga gatttcaccg catagtccct tttttatcgc tgcgttgcta
     2761 aatattgtca ctttccttgt ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat
     2821 aatacagata ccgaagtagg ggttgagacg caatcgaatt cggtatacat cactttattt
     2881 aaaacgatgc ccattttgtt gattatttat ttttcagcgc aattgatagg ccaaattccc
     2941 gcaacggtgt gggtgctatt taccgaaaat cgttttggat ggaatagcat gatggttggc
     3001 ttttcattag cgggtcttgg tcttttacac tcagtattcc aagcctttgt ggcaggaaga
     3061 atagccacta aatggggcga aaaaacggca gtactgctcg gatttattgc agatagtagt
     3121 gcatttgcct ttttagcgtt tatatctgaa ggttggttag ttttccctgt tttaatttta
     3181 ttggctggtg gtgggatcgc tttacctgca ttacagggag tgatgtctat ccaaacaaag
     3241 agtcatcagc aaggtgcttt acagggatta ttggtgagcc ttaccaatgc aaccggtgtt
     3301 attggcccat tactgtttgc tgttatttat aatcattcac taccaatttg ggatggctgg
     3361 atttggatta ttggtttagc gttttactgt attattatcc tgctatcgat gaccttcatg
     3421 ttaacccctc aagctcaggg gagtaaacag gagacaagtg cttagttatt tcgtcaccaa
     3481 atgatgttat tccgcgaaat ataatgaccc tcttgataac ccaagagggc attttttacg
     3541 ataaagaaga tttagcttca aataaaacct atctatttta tttatctttc aagctcaata
     3601 aaaagccgcg gtaaatagca ataaattggc cttttttatc ggcaagctct tttaggtttt
     3661 tcgcatgtat tgcgatatgc ataaaccagc cattgagtaa gtttttaagc acatcatcat
     3721 cataagcttt aagttggttc tcttggatca atttgctgac aatggcgttt accttaccag
     3781 taatgtattc aaggctaatt ttttcaagtt cattccaacc aatgataggc a
//
LOCUS       DAPGRN010000226         3821 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPGRN010000226 DAPGRN010000000
VERSION     DAPGRN010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.230"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..444
                     /locus_tag="RHW33_004670"
     CDS             <1..444
                     /locus_tag="RHW33_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1648084.1"
                     /translation="EEEIDVMLHQNAFVIIDTLMNNVFHSNFLTQIERLKPVHVIIFS
                     PFNIKRCLGKVPVTFVPRTITIIDFVALINGSYCSVPEANVSLSRKQHQVLSCIANQM
                     TTEDILEKLKISLKTFYCHKHNIMMILNLKRINELVRHQHIDYLV"
     gene            complement(505..1533)
                     /gene="chuS"
                     /locus_tag="RHW33_004671"
     CDS             complement(505..1533)
                     /gene="chuS"
                     /locus_tag="RHW33_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HDV1648085.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNIREAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLVRFITDEN
                     TPLELKAVDAPVVQTRADATVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGYVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVAA"
     gene            complement(1582..3564)
                     /gene="chuA"
                     /locus_tag="RHW33_004672"
                     /gene_synonym="shuA"
     CDS             complement(1582..3564)
                     /gene="chuA"
                     /locus_tag="RHW33_004672"
                     /gene_synonym="shuA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent heme/hemoglobin receptor
                     ChuA/ShuA"
                     /protein_id="HDV1648086.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASESSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT          978 a          972 c          879 g          992 t
ORIGIN      
        1 gaagaagaga tcgacgtaat gttgcatcag aatgccttcg tcattattga tacattaatg
       61 aataatgtat ttcattctaa ttttctcact caaattgaac gattaaaacc tgtccatgtc
      121 attattttct ccccctttaa tattaaacgc tgcctgggga aagtgccggt gacctttgtt
      181 ccgcggacta tcactatcat tgattttgtc gcactcatca atggcagtta ctgctctgtg
      241 cctgaagcga atgtgtcact ttcgcgcaag caacatcagg tactgagctg cattgcgaat
      301 caaatgacaa cggaagatat tctggagaaa ctgaaaatat cgctaaaaac gttctactgc
      361 cataaacaca atatcatgat gatcctcaat cttaagcgga tcaatgagct ggtacgccat
      421 cagcatattg attatctggt gtgaacgatt gatcaatata aagaggccca gcaataaata
      481 gccagacctc ccgttaatta tacgttatgc agcaacgcct tccggtatca gcgaagcaat
      541 ttgcttacgc cattgcgctt gctcctgttc gccttctgta cgttgaccat aaagttgcgc
      601 tatctgcgta ccatcatggg caaacaattc cagactggtt acgtagccat cgctggtcgg
      661 tttacgggta acccaggctt cagcaatgct ctcttctaat agatgaagag taaacgtcgg
      721 gttgaaaata ttcagccaac ctttcattgg caccactttt tctaccacac cagtgaaaat
      781 ctgtacgcag ccacggttgc caacaaacac catgatttca ttaccatcct gctgtgcaga
      841 ttcaagaatt tgcgccaacg cactgttgga tactttgcag gccaaatcgt ctgccaccag
      901 attgaacgcc tgttggcgcg tcagattgtg gcgcttgagc aacgtaaaaa actgatgaac
      961 gtcggtcatc gcccgccact cttgctcgac cacagtggca tcggctcgcg tttgaacaac
     1021 tggcgcatca acggctttta actcaagcgg cgtattctca tcggtgataa accgtaccag
     1081 aagctcactc catgccgcca tatcggtatt gtcggtggca taaactttta gtaatgcatc
     1141 gccctgatga tcaaagaact gaatactctg gcgttcgcca cgagccgtgt tttctttgat
     1201 gtggaaaaca ctggcccatt gattgagaaa cagacgtaaa tccagcgcgc gtggattgag
     1261 gatcaatccg gcatgtccgt tcaaatgctg gtttgtgaac gcaccaactt gctcatggac
     1321 tgcatattca ttacggcaaa tacatttggt ctcgccaaca ctttcgagcg ccgccagaat
     1381 ttcacggata tcgccgcgca tccgccatgc atcgtgcgtg acgcgtgcaa atgccagttc
     1441 tgcttctcta atattcatta accctgcgat gtcacgcgcg tactttccgg gattttgttc
     1501 ttttaactca agccagcgtg tgtagtggtt cattgtctct cttccttcca gataaatgcc
     1561 ccgaaatatc ggggcagatg attaccattg ataactcacg aaaatttttc cgttacgacc
     1621 atcctgtggg atgccttgtg gcgaccagta ctctttgtcg aaagcgttac ccaacactaa
     1681 agtagtggtc atacctttga gcgcctgttg tccttgataa ctgacgtaga aatcattcac
     1741 gccatagcct ggttgtttgc tgtaactgct gctgatatgt gttgagcgat cggcaaacgt
     1801 accaacccac ccaacagaga agccactgtg agcgatcgga atattcaggg tgctggtaac
     1861 ggtatccggg ttaatgctgg agatgtattc gccggtatcg gtgtctttgc cgcgggtacg
     1921 gttataggcc acatcaaggc taaacagatc agtggtatat ttcgtcatca catcccagcc
     1981 ccagattttg gcgttcggga cgttatacga catagtcgtc gccgccgcga aatcgacggt
     2041 cgtggagatg taatccttcg ctttggtatc aaagtagctg gctttaaatt ccagagcatc
     2101 attggacaac atcaggtcat caaaacgcag cccaaaaccg tactcctgag tttcgttagt
     2161 ttccggacgt aagttcgggt ttggcaccca atagttggta tagaagcgac caatcgagaa
     2221 gtgcttagaa tcgttataca tttcgcccat cgtcggggcg cggaatgcct gggcatatga
     2281 gccaaataac atcagccagt tagtcggatt gatagtcatc cccgcacgag atgaccattt
     2341 gtcggcatca acatctttgt aaccgtcact gctaccgcga taactgtcat agcgggttcc
     2401 gccaagcagg gtaatcggca gatcgcgtaa ggtgatctca tcctgtagcc agccggagct
     2461 aaaatcgatt tttgcttgcg ggaagcccgt cgtcgcgccg cccggatgtt gttcctgacg
     2521 ataatactca ccgccatatg tcagtaagtg agaagcgaaa ctgtcggcaa agagagtgga
     2581 acggttctcc agcctggctc cttttgttat ctgttcacga tactcgccgg aactccccgt
     2641 gttttgcgca ttaatacgga cttccgacca ataaattttt gcatctgcat ttaaccagtc
     2701 gttgccctgc ggggcgagtt tataagaaag ctgcgcatcg cgttgaattg ttgaacgatc
     2761 aaccatcggg ttgctgcttt cagaagcctc aacggtctgc ggattttttg gttcacgcgc
     2821 gtcgttgttg tagtaacgca ctaaaccgct cagagactgt gctgaatcaa tttgccaggt
     2881 ccccttcgcc agcatgttat taatggactc gtcattcggc gcggtttcac cattgctctg
     2941 gcgtaaatca ccccgatcgc gactggacca ggccacaata ccatccagat tttcagttcg
     3001 cccaaacgcg ctcgcgccta atcccaggct atggtccccc gtgccgccag taccaaagac
     3061 acgaaaacca ctgctttgtc cttcctgcaa taaatctttt gcatcgaccg tatcgtagga
     3121 gatcactcca cccagcgcgc cactgccata cagtaatgct gaaggtccac gaacaatctc
     3181 aacacgcttg atcagcgccg gatcgagaaa agtaccattc aggtgtccgg tatccgttcc
     3241 ctgacgaaca ccatcgacaa gaaccagcac gccgcgatga tcatagccac gcatatttac
     3301 atcctgaccg ttggttcgtc cggtaccatc cagagtaatt ccaggaacat gacgcagcag
     3361 atcggtggct gaagtagccg tttgattttc aggagcggaa gtgtcgatga cgctgaccat
     3421 cataggcgct tcgaaggaac tacgggcatt ccccgttgcc gtaacggtca tggtttcagt
     3481 agcaaaagca aacgttggca aggtggcaga aacagccaaa gccaacaaac tcaaacgcaa
     3541 cgaggtaaat tgcggacgtg acatagcgat tctccatgag gaaaataaaa aatgctggct
     3601 gcctgggtta ataccggggt ggctggcgaa aaataaattt atttaaaatt caaagcgata
     3661 atattcgccg ggtgagttta caggcgttta tatcccatga aattgcctgg tagctcggca
     3721 attacgcaaa agcacgccct ctttgtcagg caaaacgata tgccccatca ggagtgaaga
     3781 gcgctgtgcg ttatcccttg caaatgctat ggctttgtta t
//
LOCUS       DAPGRN010000227         3739 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPGRN010000227 DAPGRN010000000
VERSION     DAPGRN010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.231"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>3739
                     /locus_tag="RHW33_004673"
     CDS             <1..>3739
                     /locus_tag="RHW33_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV1648087.1"
                     /translation="GGPIVQGSAGVRIGAPTGVACSVCPGGMTSGNPVNPLLGAKVLP
                     GETDLALPGPLPFILSRTYSSYRTRTPAPVGIFGPGWKAPSDIRLQLRDDALVLNDNG
                     GRSIHFEPLLPGEAVYSRSESLWLVRGGKATQPDGHTLARLWASLPPDIRLSPHLYLA
                     TNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGRTLTYRREAAGDLAGE
                     ITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDRSRPLSASAFPDTLPGTEYGPDR
                     GIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSGTQVRAFTYDAQHPGR
                     MVGHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNRREVLHTEG
                     EGGLKRVVKKELADGSVTRSGYDPVGRLTAQTDAAGRRTEYGLNVVSGDITDITTPDG
                     RETKFYYNDGNQLTAVVYPDGLESRRAYDEPGRLVSETSRSGDVIRYAYDNPHSELPA
                     TTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDN
                     RGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTTQGGLTRSM
                     EYDVAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGL
                     VTLWYYDESDRITHRTVNGEPAEQWQYDGHGWLTDISHLSEGHRVAAHYGYDDKGRLT
                     GERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGG
                     TPLLEFTRDRLHRETVRSFGSMAGSNAAYELTSTYTPAGRLQSQHLNSLVYDRDYGWN
                     DNGDLVRISGPRQTREYVYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPD
                     STLTVWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSQHRLV
                     FHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRL
                     TTVQTDTTRIQTVYEPGSFSPLIRIETENGEREKAQCRSLAEKLQQEGSEDGHGVVFP
                     AELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDH
                     RGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNR
                     YYDPLQGRYITQDPIGLEGGWSLYAYPLNPVNGIDPLGLSPA"
BASE COUNT          782 a         1075 c         1296 g          586 t
ORIGIN      
        1 atggcggtcc cattgtccag ggttcggcag gtgtaagaat tggcgcgcca actggcgtgg
       61 cgtgctcggt gtgtccgggc gggatgactt cgggcaaccc ggtaaatccg ctgctggggg
      121 cgaaggtgct gcccggcgag acggaccttg cgctgcccgg cccgctgccg ttcattctct
      181 cccgcaccta cagcagctac cggacccgga cgcctgcgcc ggtggggatt tttggccccg
      241 gctggaaagc gccttctgat atccgcttac agctacgcga tgatgcactg gtactcaacg
      301 acaacggcgg acggagcatt cactttgagc cgctgctgcc gggtgaggcg gtgtacagcc
      361 gcagcgagtc cctgtggctg gtgcgtggcg gtaaggcaac gcagccggac ggccatacgc
      421 tggcacggct gtgggcgtcg ctgccgccgg atatccggtt aagcccgcat ctttacctgg
      481 cgaccaacag cgcacagggg ccgtggtgga tactggggtg gtctgagcgg gtgccgggtg
      541 ctgaggacgt actgccagcg ccgctgccgc cgtaccgggt gcttaccggg atggcggacc
      601 gcttcgggcg gacgctgacg taccggcgtg aggccgccgg tgacctggcc ggggaaatca
      661 ccggcgtgac ggacggtgcc gggcgggagt tccgtctggt gctgaccacg caggcgcagc
      721 gtgcggaaga ggcccgcacc tcttcgctat cttcttctga ccgttcccgc cctctctcag
      781 cctcagcgtt ccccgacaca ctgcccggta ccgaatacgg ccccgacagg ggtatccgcc
      841 tttcggcggt gtggctgatg cacgacccgg catacccgga gagcctgccc ggagcgccac
      901 tggcgcggta cacgtatacg gaagccggtg aactgctggc ggtatatgac cgcagcggga
      961 cacaggtgcg cgctttcacg tatgacgcgc agcacccggg ccggatggtg gggcaccgtt
     1021 atgcgggaag gccggagatg cgctaccgct acgacgatac cgggcgggtg gtggagcagc
     1081 ttaacccggc aggcctgagt taccgctacc agtatgagca ggaccgcatc accgtcaccg
     1141 acagcctgaa ccggcgtgag gtgctgcata cagaaggtga aggcgggctg aaacgggtgg
     1201 tgaaaaaaga actggcggac ggcagcgtca cgcgcagcgg gtatgacccg gtggggcggc
     1261 ttacggcgca gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtcag
     1321 gcgatatcac ggatatcacc acgccggacg ggcgggagac gaaattttac tataacgacg
     1381 ggaaccagtt aacggcagtg gtgtatccgg acgggctgga gagccgccgg gcatatgatg
     1441 aaccgggcag gctggtatcg gagacatcgc gcagcgggga cgtcatccgg tatgcttatg
     1501 ataatccgca cagtgaatta ccggccacga caacagatgc gacgggcagc acccggcaga
     1561 tgacgtggag ccgctacggg cagttgctgg cgttcaccga ctgctcgggc taccagaccc
     1621 gctatgaata cgaccgcttc ggccagatga cggcggtcca ccgcgaggaa ggtatcagcc
     1681 gttaccgccg ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg
     1741 aaacgcagta tgagtacaac gccgcaggcg acctgactgc cgttatcact ccggacggca
     1801 accggagcga gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc
     1861 tgacgcgcag tatggagtac gatgttgccg gacgtgtcat cagtctgact aacgagaacg
     1921 gcagccacag tgatttcagt tacgatgcgc tggaccgcct ggtacagcag ggcggctttg
     1981 acgggcggac gcagcgttat cattatgacc tgacaggaaa actcacgcag agtgaagatg
     2041 aggggcttgt caccctctgg tactacgatg aatcggaccg tatcactcac cgcacggtaa
     2101 acggtgaacc ggcagagcag tggcagtatg acggccacgg ctggctgaca gacatcagcc
     2161 atctcagtga aggccaccgt gtcgctgccc actacggcta cgacgataag ggccgcctga
     2221 ccggcgaacg ccagacggtg gagaacccgg agacggggga actgctgtgg cagcatgaga
     2281 cgaaacacgc atacaacgaa caggggctgg caaaccgcgt cacgccggac agcctgccgc
     2341 cggtggagtg gctgacgtat ggcagcggtt acctggcggg aatgaagctg ggcgggacgc
     2401 cgctgctgga gttcacgcgc gaccgcctgc accgggagac ggtgcgcagc ttcggcagca
     2461 tggcaggcag taatgccgca tatgaactga ccagcacata cacccccgca ggccggttac
     2521 agagccagca cctgaacagc ctggtgtatg accgtgacta cgggtggaat gacaacggcg
     2581 acctggtgcg catcagcggc ccgcgacaga cgcgggaata cgtctacagc gccacgggca
     2641 ggctggagag tgtgcgcacc ctcgcaccgg acctggacat ccgcatcccg tatgccacgg
     2701 acccggcggg caaccggctg ccggacccgg agctgcaccc ggacagtaca ctcacagtgt
     2761 ggccggataa ccgcatcgcg gaggatgcgc actatgtcta ccgccacgat gaatacggca
     2821 ggctgacgga gaagacggac cgcatcccgg cgggtgtgat acggacggac gacgagcgga
     2881 cccaccacta tcactacgac agccagcacc gcctggtgtt ccacacgcgg atacagcatg
     2941 gcgagccact ggtcgagagc cgctacctct acgacccgct gggacggcga atggcgaaac
     3001 gggtatggcg gcgggagcgt gacctgacgg ggtggatgtc gctgtcgcgt aaaccggagg
     3061 agacatggta cgggtgggac ggcgacaggc tgacgacggt gcagactgac accacacgta
     3121 tccagacggt atacgagccg ggaagcttct caccgctcat ccgcatcgag acagagaacg
     3181 gcgaacggga aaaagcgcag tgccgcagcc tggcggagaa gttacagcag gaagggagcg
     3241 aagacggcca cggtgtggtg ttcccggctg aactggtggg gctgctggac aggctggagg
     3301 gggaaatccg ggcaaactgc gtgagcagtg aaagccgcca gtggcttgcg cagtgcgggc
     3361 tgacggtgga gcggctggcg gcacagatag agccggttta tttgcctgag cgaaaaatac
     3421 atctttatca ctgtgaccac cggggcctgc cgctggcgct tatcagtgaa gacggcaata
     3481 cggcgtggag cgcggaatat gatgaatggg gcaaccagct taatgaggag aacccgcatc
     3541 acctgcacca gccgtaccgg ctgccggggc agcagtatga taaggagtcg gggctgtact
     3601 acaaccggaa ccggtactac gatccgttgc aggggcggta tatcactcag gacccgatag
     3661 ggctggaggg gggatggagt ctgtatgcgt atccgctgaa tccggtgaat ggtattgatc
     3721 cattagggtt aagtcccgc
//
LOCUS       DAPGRN010000228         3613 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPGRN010000228 DAPGRN010000000
VERSION     DAPGRN010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.232"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..378
                     /gene="traK"
                     /locus_tag="RHW33_004674"
     CDS             <1..378
                     /gene="traK"
                     /locus_tag="RHW33_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV1648088.1"
                     /translation="VSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVP
                     VTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALSIRESDFWQPGTRAVMFS
                     QPASQLLAGARMDVYVIRDGEGN"
     gene            378..1829
                     /locus_tag="RHW33_004675"
     CDS             378..1829
                     /locus_tag="RHW33_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDV1648089.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGYD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            1798..2379
                     /locus_tag="RHW33_004676"
     CDS             1798..2379
                     /locus_tag="RHW33_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDV1648090.1"
                     /translation="MPARRAEMAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMAL
                     FVLWKDNTTPGKLLVQEIDFVRQTAPAGQFPVRECLPSLHDLPGAEEKICRYRAADAA
                     DYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPVRPGKMKCVRVATADERLKEVL
                     AEDTSLPGKIRECHVYFPDDRTIRNNGDKNEHA"
     gene            2318..2686
                     /locus_tag="RHW33_004677"
     CDS             2318..2686
                     /locus_tag="RHW33_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2689 domain-containing protein"
                     /protein_id="HDV1648091.1"
                     /translation="MFISLMTEQSETTEIKMNMRNINVITARSVPDKSVSDDLMHAVL
                     SNCTTRIVLPAPKEFSSESLPHNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGSGKS
                     VPVKELIDRIIQKYKEPFDE"
     gene            2679..2930
                     /gene="trbG"
                     /locus_tag="RHW33_004678"
     CDS             2679..2930
                     /gene="trbG"
                     /locus_tag="RHW33_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HDV1648092.1"
                     /translation="MNKLVSDGSVKKIKYPVLYESGITPPLCEVSVPEPDAGGKRIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFVNCDEKGEGQ"
     gene            2927..3442
                     /gene="traV"
                     /locus_tag="RHW33_004679"
     CDS             2927..3442
                     /gene="traV"
                     /locus_tag="RHW33_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDV1648093.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLGEGNFRTMPVQTVTAATPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTIVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
BASE COUNT          893 a          866 c         1066 g          788 t
ORIGIN      
        1 gtcagtgacc tgcgcggaac cggagaagaa gccggtgcgt gggaaacgtc cacgccttac
       61 gaatccctgc ttgtgaccat cagccaggcc gtccgtggcg gaaaattacc cgcaggctgg
      121 tatcaggtcc cggtgacaaa ggaaaccctg caggccccgg cgggtctgtc ttcagtggca
      181 gatgccgtat ggacggggaa tcacctgaag atggtccgct ttgccgtgga aaataaaact
      241 ctgtctgctc tgagtatccg ggaaagtgac ttctggcagc cgggaacccg tgccgtgatg
      301 ttcagccagc ctgccagcca gttactggca ggtgcgcgca tggatgtgta tgtcatccgt
      361 gacggggagg gcaactgatg gccagtatca ataccattgt gaaacgcaag cagtacctgt
      421 ggctggggat tgtggttgtc ggtgcagcct ccgcgattgg tggtgcgctg tatctgtctg
      481 atgtggacat gtccggtaac ggtgaaaccg tggctgaaca ggagcctgtg ccggatatga
      541 ccggtgtggt ggatacgacc tttgatgaca aggtgcgtca gcatgctact acagagatgc
      601 aggtgacggc ggcacagatg cagaagcagt atgaggaaat ccgtcgtgaa ctggatgtcc
      661 tgaacaaaca gcgcggttat gaccagcgtc gtattgaaaa gctgggacag gacaatgctg
      721 cactggcaga gcaggtgaaa gccctgggtg caaatcccgt caccgcgacc ggtgagcctg
      781 taccgcagac gccagcctca ccgcccgggc cggaaggcga accacagcca ggaaacaccc
      841 ccgtatcctt cccgccgcag ggcagcgttg ctgttccacc gccgacggcg ttttatcccg
      901 ggaatggtgt cacgccacca ccacaggtga cgtaccagtc tgtgccggtg cctaaccgga
      961 tacagcgtaa ggtgtttacc cgtaatgagg gaaaacaggg accatcactg ccgtacattc
     1021 cgtcaggaag ttttgcgaaa gccatgctga ttgaaggggc ggatgccaat gcctcagtca
     1081 ccggtaatga atccacggtg ccgatgcagc tgcgtatcac cggcctggtg gaaatgccga
     1141 acagcaaaac gtatgacgca acgggatgtt ttgtggggct ggaagcctgg ggggatgtct
     1201 ccagtgagcg tgccattgta cgtacccgca atatcagctg cctgaaggac ggcaaaacca
     1261 ttgatatgcc gattaagggg catgtcagct tccggggtaa aaacggtatc aagggcgaag
     1321 tggtgatgcg taacggcaaa atcctcggct gggcatgggg cgcgggattt gttgacggta
     1381 tcggtcaggg aatggagcgc gcctcccagc cggctgtcgg gctgggtgcc acagccgctt
     1441 acggggctgg tgatgtcctg aaaatgggta tcggtggcgg cgcatcgaaa gccgcacaga
     1501 cgctcagtga ctactacatc aaacgtgccg aacagtatca cccggtgata ccgattggtg
     1561 cgggcaacga agtgaccgtg gtgttccagg acggcttcca gctgaaaacc gtggaggaaa
     1621 tggcgctgga gcagtcacag agtacagcgg aagagggtaa cccggaaagc ccggtgccga
     1681 atcctccctc ggcagaaagc cacctgaacg gttttaacac cgaccagatg ctgaaacagc
     1741 tgggcaatct gaatccgcag cagtttatgt ccggagacaa gaacccgctg ggcggggatg
     1801 ccggcaagga gggcggaaat ggcgggtgat gcactgacat tccgtgtggc gtttgtcgtt
     1861 cgctggctgc tgtgggtcgt caggtttgtg gttatctggc cactggccac aatggccctg
     1921 atggctctgt ttgttttatg gaaggacaac accacaccag gaaaattact ggttcaggaa
     1981 attgacttcg taagacaaac agcccctgcc gggcagtttc ctgtaagaga atgtttgcct
     2041 tctctgcatg atcttccagg agcagaagag aaaatctgcc gttaccgtgc tgctgatgct
     2101 gcagattatg tcagggagac tgaccggtca ctgatgcaac tggtcaccgc attctgggca
     2161 acgctggcac ttatgtatat gtcgtttgcg gcaggcaccg ggaaatatcc ggtgcgaccc
     2221 ggaaaaatga aatgtgtccg tgtggcaaca gcagatgagc gtctgaagga agttcttgca
     2281 gaagacactt ccctgccggg aaaaatcagg gagtgccatg tttatttccc tgatgacaga
     2341 acaatcagaa acaacggaga taaaaatgaa catgcgtaat attaatgtta ttactgcccg
     2401 ctctgtaccg gataaaagcg tatcagatga tcttatgcat gctgttctca gtaactgcac
     2461 cacaagaatc gtacttcctg caccgaagga attcagttct gaatcattgc cgcacaattt
     2521 taatatggct gctgtcggtg tgatgaagaa gggtatgtcg atgagcttat tttcttctcc
     2581 ggggcatatt gtggttgccg gacaatccgg tagcggaaaa tctgttccgg tgaaagaact
     2641 aatcgacaga attattcaga aatataagga acccttcgat gaataagctg gtttctgatg
     2701 gcagcgtcaa aaaaataaag tatccggtgc tgtatgaatc cggaataacc cctccgttat
     2761 gtgaagtcag tgtgccagaa cctgatgcgg gggggaaacg cattgtggca tatgtttata
     2821 aatcatcacg gaatacggtt tttgaaaatc ctgacattgt aaaaacctgt acggtcaggg
     2881 atttgaagaa tgattttgtg aactgcgatg agaaaggtga gggacaatga aacagatttc
     2941 tttatttatt cctctgctgg ggaccttatt actttccggc tgtgccggaa ctaatacgga
     3001 atttgagtgt aacgccacca catccgatac ctgtatgacg atggagcagg ccaatgagaa
     3061 ggccaaaaaa ctggagcagt cctcagaagc aaagccggtt gcggcatcac tgccgcgcct
     3121 gggtgaaggg aacttccgga caatgccggt gcagaccgtc accgcggcca ctccgtccgg
     3181 cagcagacct gcggtgacag cacatccgga gcagaaactg ctggcaccac gcccgctgtt
     3241 taccgctgcc cgggaagtga aaacgattgt tccggtcagt tcagttgcgc cggtaacacc
     3301 tcctcgtccg ctaagaacgg gtgagcagac ggctgcatta tggatagcgc cttatattga
     3361 taatcaggat gtttatcatc agccatccag cgtgtttttt gttattaaac cgtctgcgtg
     3421 gggaaaacca cgtattaatt aactggccct gaagttgtat atcagccggc aatgccggca
     3481 ggctttctgt attcaaaatc tgcctgtaaa acgagtgccc tgtgcgtgaa aagggatggg
     3541 cggtagcgtg tcagaacatg tcgggaggaa tattccgtga gtgatgaagc cgatgaagca
     3601 tattcagtga cag
//
LOCUS       DAPGRN010000229         3610 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPGRN010000229 DAPGRN010000000
VERSION     DAPGRN010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.233"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            4..1974
                     /locus_tag="RHW33_004680"
     CDS             4..1974
                     /locus_tag="RHW33_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDV1648094.1"
                     /translation="MYMNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            1981..2772
                     /locus_tag="RHW33_004681"
     CDS             1981..2772
                     /locus_tag="RHW33_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDV1648095.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGVQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLQVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
     gene            complement(2835..3434)
                     /locus_tag="RHW33_004682"
                     /pseudo
     CDS             complement(2835..3434)
                     /locus_tag="RHW33_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            3434..3610
                     /locus_tag="RHW33_004683"
                     /pseudo
     CDS             3434..3610
                     /locus_tag="RHW33_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          948 a          891 c          907 g          864 t
ORIGIN      
        1 cgcatgtata tgaacgtaat cagaaacgtc atttgtacat taatcatact cccgataggg
       61 ttacaggcag agaccagtca ttcttctatg gctaaagata caatcaccgt tgtcgcgaca
      121 ggaaatcaga acacggtatt tgaaacgccg tcaatggtca gtgtcgtcac gaatgacacg
      181 ccatggagtc agaatgcggt cacatcagcc ggcatgctga aaggtgttgc cggtctcagc
      241 cagactggtg caggacggac caatgggcag acctttaatc tacgcggcta tgacaaaagc
      301 ggggtacttg ttcttgttga cggcatccgt caactcagtg acatggcaaa aagcagtggc
      361 acttatctgg atccggcact cgtcaaacgt atcgaagttg tccgcggacc aaactccagt
      421 ctgtacggca gtggcgggct gggaggtgtt gtggacttca gaactgccga tgcagcagat
      481 ttccttcccc ccggagagac aaacggttta agtctgtggg gaaatatcgc cagtggtgac
      541 cacagcacag gctcggggct cacctggttc ggtaaaaccg gaaaaacaga tgcgctcctt
      601 tctgtcatta tgcgtaaaag aggtaatatc tatcaaagtg atggtgagcg cgcacctaac
      661 aaggaaaaac ctgcagccct gtttgcgaaa ggctctgtcg gtataacaga cagtaacaaa
      721 gcaggtgcca gcttgcgtct ctaccggaat aacaccactg aaccgggcaa tcccactcag
      781 acacatggtg acagcggcct gcgtgacaga aaaacagtac aaaatgacgt acagttctgg
      841 taccagtacg ctcctgtgga taacagcctc atcaatgtaa agtcaacttt atatctcagt
      901 gatatcacta tcaagacaaa cggtcacaac aaaacggcag aatggagaaa caacagaacc
      961 tccggtgtta atgttgtcaa caggagtcat actctgattt ttccgggagc ccatcagtta
     1021 agttatggcg ctgaatatta ccgtcagcag cagaagccag aaggctctgc cacactatat
     1081 ccggaaggaa acattgactt tacatcgttg tatttccagg atgaaatgac aatgaaaagc
     1141 tacccggtta acattatcgt cggttcccgc tatgaccggt acaagagctt caatccccgt
     1201 gccggagaac tgaaagccga acgcctgtcc ccaagggcgg cgatttcagt ctcaccgaca
     1261 gactggctga tgatgtacgg ctccatatcc tctgcattcc gtgcccccac aatggcagaa
     1321 atgtacaggg atgatgtaca tttttaccgc aagggtaaac ccaattactg ggtacctaac
     1381 cttaatctga aaccagaaaa taacattacc cgtgagattg gcgcaggtat tcaactggac
     1441 ggcctgctta caggcaatga tcggctgcag ttgaaaggcg gatatttcgg aacggatgcc
     1501 agaaactata ttgccacgcg cgtggatatg aaacggatgc gttcttattc ttataatgta
     1561 tcccgggccc gtatctgggg atgggatatg cagggaaatt accagtctga ttattttgac
     1621 tggatgcttt cttataaccg cacggaaagt atggatgcca gcagcaggga atggctgggc
     1681 tccggcaatc ctgacacact catcagtgac atcagtatac ctgttggtca tggaggagtt
     1741 tctgccggat ggcgtgctga actttcggca gcagccacgc atgtgaaaaa aggcgatccc
     1801 catcaggctg gctatgccgt acattccttt tcactgtctt ataagcctgt aagtgttaaa
     1861 ggctttgagg catcagtgac tctggataat gccttcaaca agcttgccat gaatggcaaa
     1921 ggagtgccgc tttcaggcag aactgtcagt ctttataccc gttatcagtg gtaacaaatc
     1981 atgatgaaaa atacaggtta tatcttagct ctttgtctga cagcatcggg gcatgtcctg
     2041 gcccacgatg tctggattac aggtaaacag gcagagaaca acattaccgc agagattggt
     2101 tatggtcata atttcccctc aaagggcaca attcctgaca gaaggaattt ctttgaaaat
     2161 ccccggattt ataacgggaa agagacaata acactgaagc cagcgtccac ggattatgtc
     2221 tataaaactg agtctgcaag caaagataat ggctacgttt tgtcaacata tatgaaaccg
     2281 ggatactggt cgagaacctc ttcaggatgg aaaccggtca gccgggaggg cagaaatgat
     2341 gtggcttact gtgagtttgt cactaaatat gcgaaatctt ttattcctgg tgtacagcag
     2401 atgccagcac aactctatca gtctccaacg gggcatgagc ttgaaatcat tccgttatcc
     2461 gatataagtc gtttcagtga agatgtgaag ctgcaagtcc tgtataaaac gtccccgctc
     2521 gccggagcta tcatggagct tgactcggtc agttatctga aatcatcccg tcatactcat
     2581 gcagttgagc acaaacatcc tgttcataaa gcagaactca cctttgtaac taatgaggat
     2641 ggtatcgtca cagtaccttc tcttcatatt ggacagtggc tggcgaaagt ccaaaataag
     2701 aaaagttttc aggacaaaag cctgtgtgat gaaactgtcg atgtggcaac tttaagtttc
     2761 tcccgaaatt aacaatacta ttccctgccc tgaaattgta agcgtgcagc aagaaccgta
     2821 ttgacgggga tgtgttattc agtcggcagt gctacgcgcc aggggagcag ttcgccgacc
     2881 cggtttatcg gccagtcggc tatgacgtca aggacatagc ggaggtagct ttctggctcc
     2941 actccattca gtttgcacgt cccgatcagg ctgtacagca gcgctccccg ctctcctcca
     3001 tgatccgaac cgaagaacag gtagtttttg cggcccagac tgaccatccg caacgcattt
     3061 tcagcgatgt tattgtccgc ctcagcccag ccatcatctg catagtacgt cagcgccggc
     3121 cactggttca gggcgtatgc gaacgctttc gccagttctg agtgtcgcga cagggttttc
     3181 atcttttcac gcagccagct ttccagggat ttcagcagcg gtttcgtttt caactgacgt
     3241 tcggcaaggc gctgctccgc cgtcattccc cttatctctg cctcgatggc gtacagttcg
     3301 ccgatccgtt tcagcgcttc ctccgtcagg gctgacgggg tgcgaacgtg cacatcgtgg
     3361 attttacggc gggcgtgagc ccaacaggcg gcttccgtta tccggccatc ccggtacagc
     3421 tcgttgaacc cggcgtcagt tgttacggga aggaatcagg gtagccagat gtacagtggc
     3481 gcgcctcatg gcggttatgg gacttgccga tgttctccgg ggtaaaaagg tccgcactac
     3541 cgtcagccgg aaagccgttg ccgcaggcga ccgcgtaaac cgtcagtttg tggcagaacg
     3601 tcctgaccag
//
LOCUS       DAPGRN010000230         3456 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPGRN010000230 DAPGRN010000000
VERSION     DAPGRN010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.234"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..692
                     /locus_tag="RHW33_004684"
     CDS             <1..692
                     /locus_tag="RHW33_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV1648096.1"
                     /translation="NVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAV
                     RRTLKEEEKLVNSSDWGYDTQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQIG
                     GKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTL
                     LFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAK
                     QIARLAEQSTD"
     gene            791..1804
                     /locus_tag="RHW33_004685"
     CDS             791..1804
                     /locus_tag="RHW33_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV1648097.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="RHW33_004686"
     CDS             complement(1801..2631)
                     /gene="amiD"
                     /locus_tag="RHW33_004686"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV1648098.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     STWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQGRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2628..2951)
                     /locus_tag="RHW33_004687"
     CDS             complement(2628..2951)
                     /locus_tag="RHW33_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV1648099.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(3162..>3456)
                     /locus_tag="RHW33_004688"
     CDS             complement(3162..>3456)
                     /locus_tag="RHW33_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1648100.1"
                     /translation="NMNAGSNVKIAADAFLDILFSLKNKYCIKLLSAQDRAFIYEFAR
                     MIFAYINDKNIENLLLSCFAEVDQKAIQRYVPQPQDAEKFWSNLQYKLSISAH"
BASE COUNT          817 a          932 c          865 g          842 t
ORIGIN      
        1 tcaatgtgat tacttccgta ccgcccacca ccgccagggc gttgattcag gggctgaaac
       61 acgatctgct ggcggatgac acagcgctac gtgcactcat cccacaacgg ctgatcgctt
      121 tcgatgacgc ggtacgtcgc accctgaaag aggaagaaaa gctggtcaac tccagcgact
      181 ggggctacga cactcaggcc tttgcccgct ggcgacctga gtacggttat ttcgccaaac
      241 aggcggggtt taccgttaaa acgtccgcca gccttgcggc tttatggcag gtggtgaacc
      301 aaatcggcgg taaagagcgt tatttctttg gcaatatttt gtggcagaca cgggcgttga
      361 tggaccgtgc gatcggtcat aagctggcga aaggccgtcc ggagcgcgaa tatttgcaaa
      421 ctggcgatgc ggtggatagc tggaaagtga ttgtcgttga accggaaaaa caacttacgt
      481 tgttatttgg catgaaagca ccggggctgg gacgactgtg ttttaccctg gaagataaag
      541 gcgactatcg tactatcgat gtccgcgctt tctggcatcc gcacggtatg ccggggctgt
      601 tttactggtt attgatgatc cccgcgcatc tgtttatttt tcgcggaatg gcaaaacaaa
      661 tcgccagact ggcagaacaa agcacagatt aatcgacaaa acctgcgtta ttctttcatg
      721 tttcccattg catcatgggg gaaatcacgg aagaatgcgc acgtcatttt ctcaacaaca
      781 gtggattgat atgaaggtac tggttaccgg cgccaccagc ggcttaggtc gaaacgcggt
      841 agagttttta tgccagaaag gcatcagcgt gcgagcgacc ggtcgcaacg aggcaatggg
      901 taaattgctg gaaaaaatgg gcgcagagtt tgttccggcg gatctgaccg agctggtctc
      961 atcacaagct aaagtgatgc tcgcgggcat tgatacgctg tggcactgct ccagctttac
     1021 ctcgccctgg gggacacaac aggctttcga tctggctaac gttcgcgcca ctcgccgcct
     1081 gggtgaatgg gctgtcgcct ggggtgtgcg taactttatt catatctctt ccccctccct
     1141 gtacttcgat tatcaccacc atcgcgatat taaagaagat tttcgccctc accgcttcgc
     1201 caacgagttt gcccgcagca aagcggccag cgaagaagtg atcaatatgc tttcgcaggc
     1261 gaatccacaa acgcgcttta ctattctgcg cccacaaagt ctgttcggac cgcacgataa
     1321 agtctttatc ccccgtctgg cgcatatgat gcaccactac ggcagcattc tgttaccgca
     1381 cggcggaagt gcgctggtcg atatgaccta ctatgaaaat gcagtgcacg ccatgtggct
     1441 ggcaagccag gaagcctgcg ataagctacc ttccgggcgt gtgtacaaca tcaccaacgg
     1501 cgagcatcgc acactgcgca gcatcgtgca gaagctgatc gacgagttga atattgactg
     1561 tcgtattcgt tccgtcccct acccgatgct ggatatgatc gcccgcagca tggagcgttt
     1621 aggtcgcaag tcagcaaaag agccgccgct gacccactac ggcgtctcca agttgaattt
     1681 tgactttacg ctggatatta cgcgtgcaca ggaagaatta ggttatcagc cggtcatcac
     1741 cctggatgaa ggtatcgaga aaactgccgc ctggctacgc gaccatggaa aactgccgcg
     1801 ctaatcctgc ccgtatttct ccagcaacgc ttcggcaatt gcctgagttt ctgcatccgc
     1861 ttcgccgttg tataacgtcg ggcggaaatg catctggaac gccataatca cgcgcccctg
     1921 ctcgcgcggt gtcatatcag gtttaacgtc ataaccgtag cgcgccaaaa gctccagcaa
     1981 tgacgcggtt tcaaccggtg tgtgcggtgc acgcccggca aggtaaaagt tcacccgctg
     2041 cgcatccggc caggcaccaa tcccctgctg cgccagttgc tgccagggaa ataatggccc
     2101 cggatcgtct ttgcgctgcg gggcgatatc cgcatgtgcc actacgtttt ccggcttgat
     2161 gtgataacgg gcaataatat ctttcgccag cggaataagc gcctgaatct gtgccggttc
     2221 aaacggggca aaatatttca cccccgccga tttttgccag ccacggtttt ccagctcaat
     2281 gccaatagag gtgtcgttaa ggcgcgttgc cccgcgccag gtgctaatcc ccgcatgcca
     2341 ggccagttct tgttccggca ccagttgcca gatgcgcggt ttaccgttgt atcgcggtgg
     2401 taccgcaggg accagataat gcgagctgac ctgcttatcg gtcagtgtcg ccagcgagct
     2461 atcaaaatca tctgcggtgt agtggatcac cagcacttta atgcgcggat acgccgcctg
     2521 cgcctggcgt cgggtatcaa gctggtatcc ctctttctcg acaatgcctt tttcgcctgc
     2581 acaccctgcc agtaacagag cggcagcgac cagccagaag actcttctca tcgacgcgtt
     2641 ttcaccgccg taccgctgac gctaaccatc agcatactgc cgttttgccc gaccgtttcg
     2701 tagtcgatat caataccgac tacggcatcg gcccccagcg cccgcgcctg ggagccgagc
     2761 tcctcaaagg cgatctcccg cgctttacgc agttcttttt catacgcacc ggagcgtccg
     2821 ccaacgatat cgcggatacc ggcaaagaaa tcacggaaaa tattggcacc taaaatcgct
     2881 tcgccggtca ccacaccgca atattcaacg atggtctggc cttccagagt tggggttgta
     2941 gaaaattgca tgatcgatct ccttgtttgt taagccttcg atctctaaag cattatcgga
     3001 ctgatatgcc atgttgggaa gaaggttttg atcagtttag gaaatcatct cattgtgtca
     3061 aaaacaccca cttaagtctt tacgattatc cgttgcataa cccgatattg gtttcattat
     3121 agggtaccgc ttacctgtct tattatgttg taccaacaac attaatgagc ggaaatactc
     3181 aacttatact gtaaattact ccaaaatttt tccgcatctt gtggctgagg aacatacctc
     3241 tggattgctt tttgatcgac ctcggcaaaa caagaaagca ataagttctc aatgtttttg
     3301 tcattaatat atgcaaagat cattcttgca aactcgtata taaacgcccg atcctgagcg
     3361 cttaatagtt taatgcaata tttattttta agagagaaaa gaatgtcaag gaaagcatcc
     3421 gctgcaatct ttacgttact ccccgcgttc atatta
//
LOCUS       DAPGRN010000231         3434 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPGRN010000231 DAPGRN010000000
VERSION     DAPGRN010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.235"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..276
                     /locus_tag="RHW33_004689"
     CDS             <1..276
                     /locus_tag="RHW33_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1648101.1"
                     /translation="IKDVLLWIEHNLDQSLLLDDVANKAGYTKWYFQRLFKKVTGVTL
                     ASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRVMTPTY"
     gene            complement(270..542)
                     /locus_tag="RHW33_004690"
                     /pseudo
     CDS             complement(270..542)
                     /locus_tag="RHW33_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..2069
                     /locus_tag="RHW33_004691"
     CDS             666..2069
                     /locus_tag="RHW33_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV1648102.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTSAEEELLRKMLPEAPAHHPNYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2103..3317)
                     /locus_tag="RHW33_004692"
     CDS             complement(2103..3317)
                     /locus_tag="RHW33_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HDV1648103.1"
                     /translation="MFMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQNPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFSTQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTELYADIP"
BASE COUNT          928 a          805 c          819 g          882 t
ORIGIN      
        1 atcaaagatg ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat
       61 gtggcgaata aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca
      121 ggggtcacac tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg
      181 aggttgacga aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa
      241 tcttttacac gtcgggtaat gactccaact tactgatagt gttttatgtt caaataatgc
      301 ccgatgacct tgtcatgcaa ctccaccgat tttgagaacg acagtgactt ccgtcccagc
      361 cttgccagat gttgtctcag attcaggtta tgtcgctcaa tgcgctgagt gtaacgcttg
      421 ctgataacgt gcagctttcc cttcaggcgg gattcataca gcggccagcc atccgtcatc
      481 cataccacga cctcaaaggc cgacagcagg cccagaagac gctccagcgt ggccaacgtg
      541 cgttacctaa aacctgttta aatatccaga taaaaacatt caatctgggt caaatgagtg
      601 atacagtttc acccataaga cccaatggag gcaatatgtc tgaatttgaa ttactggcgc
      661 aggatctgct tgagaaagca gaagcggaag aacaactgcg acaggaaaat gataaaaagc
      721 tgctcgggca ggtgctggaa atctatgacc agaagtacgt ggctgaactg cttagaaaag
      781 ttggtaaaaa tgagtggagt cgcgagactc ttaatcgctg gattaatggt aagtgctcac
      841 ctaagacgct gacgtcagcc gaagaggaac ttctacgaaa aatgcttccg gaagcgcctg
      901 cacatcaccc taactatgcc ttccggttta ttgacctgtt tgctgggatt ggaggtatac
      961 ggaagggctt cgaaaccatc ggtggccagt gcgtttttac cagtgaatgg aataaagagg
     1021 ctgtgcgcac atataaagct aactggttta acgatgctca ggaacacact ttcaatctcg
     1081 atattcggga agtcacgctc agtgataaac ctgaagtacc tgaaaacgat gcctatgctt
     1141 acattaatga gcatgtgccg gatcatgatg tacttctagc aggtttcccc tgtcaaccgt
     1201 tcagccttgc gggcgtaagc aagaaaaact cgctcgggcg cgcgcatggt ttcgaatgtg
     1261 aggctcaggg aacacttttc ttcgatgtgg cgcgtattat ccgcgcaaaa aaacctgcca
     1321 tctttgttct tgaaaacgtt aaaaacctga agagccatga caagggtaaa acctttaaag
     1381 tcatcatgga taccctcgac gaactgggct atgaagttgc ggatgcagct gagatgggca
     1441 aaaacgatcc taaagttatc gacggaaagc actttttacc tcagcaccga gaacgtatcg
     1501 ttttggtcgg tttccgtcgt gatctgaaca ttcaccaggg ctttaccctg cgcgatatta
     1561 gtcgttttta tccggaacag cgtccgtcat ttggcgaact gctggaaccc gtggttgaca
     1621 gcaaatatat actgacgccg aaactctggg agtatctcta taactacgcc aaaaagcacg
     1681 cagctaaggg taacggattc ggttttggcc tcgttaatcc tgaaaataaa gaaagcattg
     1741 cccgtacgct ttctgctcgc tatcacaaag acgggtctga aattctgata gaccgtggct
     1801 gggatatggc cacaggtgaa acagacttcg cgaacgaaga aaatcaggcg catcggcccc
     1861 gtaggctgac tccacgagag tgcgcgcgcc ttatgggttt tgaaaaagta gatggcaggc
     1921 cttttcgcat tcctgtgtca gacactcagt cgtacaggca gttcggtaac tccgtagtgg
     1981 tgcccgtgtt tgaagccgta gccaaactgc ttgaacctta tatcctgaaa gcggttaatg
     2041 ccgattcgtg caaggttgaa cgaatctgat cgctcctccc ggtatttatg ccgggagata
     2101 atctatggaa tatctgcgta aagctctgtc agctcagcaa taaacgcacc tagcgtcatt
     2161 agctcagctc tcaccgcctc cgggtatttt ttgtgcagag atgatggcac gaccaatctg
     2221 acacccgact cccgcatctc ccgatattga gccagagaaa ctccctcctg gagtgtaaac
     2281 agatgcacct gatgaatttt atcggcctca ttcagtatct gacgccagcg atccttacag
     2341 gtagtcttga ctgccagcat gcgcagattt tctacgggaa actcagtatc gtggtaagcc
     2401 cctgcggaag ggaaaaggaa atcgggtttt ttattacctt ctgtgatggc ctgcgtcgaa
     2461 aagtgtcgca ggccgtgctc aatgaataga tgctccaggt gcagttccag cgacttcccg
     2521 gctctggatt tacggcgatt gctgacagaa ttggccagcg caataaattc atccacagag
     2581 ccaaatcctt tccggatgat atccagaaca tgcagttcct caaccaatag aaatatgtcg
     2641 tactccacgc gccggcggtc aagaagttgc tcatccggat caagggaatt tttcacataa
     2701 tggctggctg catactgaat aatttcactt cccgacggaa agcgcaggtg ccagtcttca
     2761 ggtagaatat atttatgatt tactggcgct tgctgtagag atagtccgcc tagaatctgt
     2821 cctgcggggc cggatataag cgctccgggt ataacttcac caatagcggt ctcaatgacg
     2881 tcctcttcat cagtgctggc gcatacccaa atatttactt ccttacagtc ccccccttgc
     2941 tcatcaagct tgaaagctag gagcgtcaga gcacctgtat tttcaggatt ctgaagtggg
     3001 ctgcctctac cccagcgggt aatccttttt tcattccggg ttttaccaaa atgacggctg
     3061 ttataataaa ttgcccgggc ttcgctgtca gggcaatcat gcgatgacac atgtgcggtg
     3121 agaaaaaccg aagggttcag ttcacgggta tggttgatag acggaaagag tttttcaacg
     3181 atacctgaag ggatataaag tcctacctgg tgaccacctg ttgcgccggt atcgttggcg
     3241 gaaaggcgtt tgatgtagac gaagtaattc tcacatgcga tctcgagtag ccagttgtgg
     3301 aaaaccgaca taaacatccc ctgttaccct gaaactctac tcaccatttt ttcataatta
     3361 tatacaaaca gccagaaagg ctgttacagg cgatttgatc tgcaacctat tggttaaatt
     3421 aatgtatcaa aaac
//
LOCUS       DAPGRN010000232         3427 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPGRN010000232 DAPGRN010000000
VERSION     DAPGRN010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.236"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..722
                     /locus_tag="RHW33_004693"
     CDS             <1..722
                     /locus_tag="RHW33_004693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648104.1"
                     /translation="KDMVLVGLLEDYKGLDTKVSYKCNCCDTAYETIITRIINDDAGC
                     RHCGSIKRGINKRKHTDDEWMDLIEHECRNKRLELIEQPKSPILSTSKIHIKCEKGHL
                     SDSLVVYGLVYENLGCPCCSKNGYNRSKSGVFYVQQVGDYIKYGITNHLNPQKRVTQQ
                     ARESGLKHKLLWYITFGDGKVANDLEYSVKHNIGNHAAPKELIPDGYTETCDITKEAQ
                     IRKMAMDFCKNSNNYKITYRG"
     gene            724..1077
                     /locus_tag="RHW33_004694"
     CDS             724..1077
                     /locus_tag="RHW33_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HDV1648105.1"
                     /translation="MLSKHFSRKEFKCKCGKCDYDTIDAELLVILEDVREHFGKPVII
                     NSGNRCPTHNKNVGGATNSYHVRGRAADIVVKGVSPDIVHAYLDGKYPTQYGLGKYKT
                     FTHIDSRSKKSRWNG"
     gene            complement(1230..2909)
                     /locus_tag="RHW33_004695"
     CDS             complement(1230..2909)
                     /locus_tag="RHW33_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV1648106.1"
                     /translation="MKTKLYSYIRFSSMKQADGSSYERQKRTAAEIAAKYDLELVTSY
                     QDFGVSAFKGANSKTGALSRFLDEIGRSVPVGSWLVVENLDRISRQSIIEAQELFLSI
                     IRRGITIVTGMDGRVYSRESVNANPIDLLLSVMLFARANEESQTKRNRTNSSALIKIK
                     SHQENPQNPAVAIEEVGKNMWWTDTTSGYVLPHPVYFPVVQEIVELRKNGRSTAEILD
                     HLNETYAPPPAVSNKKHSKWSRAMVERLFHTRALIGIKEVSVDGVNYELNNYYPRVID
                     DATFYHLKKNIGARAWNFGDKEEAKPIPLLSGIGLLKCDHCGCAMVKVKGTNKRSDQY
                     RYTCDATRSRRLDCEHPNWSFRGDQLEKAVLQLLADKIWIAEDKANPVPALKVQVDEI
                     SRKIDNLIALSAMTGATKELADQITTLNSERETLYNQIKMAEEEMYSVDSQGWEKLAE
                     FDLEDVYNEERLKVRFKIKQALKRIGCSRIDRNKNLFVLEYIDGKTQRVVIENSRGTR
                     KGRIFVDLKTINDRQILESNGLVLHPCLDMLTDKNWKPEEEIPGPLQEFGI"
     gene            complement(2937..3281)
                     /locus_tag="RHW33_004696"
     CDS             complement(2937..3281)
                     /locus_tag="RHW33_004696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648107.1"
                     /translation="MAKAGYLYVQHVTGEVEAIKFGITNRKPEQRMRQHSNRSKLNHE
                     IVFSMKFEDGQKALDVETRIKQIWKGKTGYVSRELMEDGFTETLPAEIMSGFLKDVKS
                     MCNLAVMNISLA"
BASE COUNT         1021 a          760 c          650 g          996 t
ORIGIN      
        1 gcaaagatat ggttttagtt ggattgttag aagattataa aggattagac acaaaagttt
       61 cttataagtg caattgctgt gatactgcat acgaaaccat aatcactaga atcataaacg
      121 atgatgctgg gtgtcgtcat tgtgggtcaa ttaagagagg aatcaataag agaaaacaca
      181 cagacgacga atggatggat ctaattgagc atgaatgcag gaacaaacga cttgaattaa
      241 tagaacaacc taaatctcct atattgtcaa catcaaaaat acacatcaaa tgtgaaaaag
      301 gtcatctttc tgattctttg gtagtttatg gtttagtgta tgaaaactta ggctgtccat
      361 gttgttctaa aaatggttat aacagaagta agtcgggagt tttctacgtt caacaggtcg
      421 gagattatat aaaatatgga ataacaaacc atctaaaccc acaaaagcga gtaactcaac
      481 aagctaggga atcagggtta aaacacaagt tattgtggta tataacgttc ggtgacggaa
      541 aagttgccaa tgacctagag tattccgtaa aacataacat aggaaatcac gctgcaccaa
      601 aagaactaat cccagacgga tatacagaaa catgtgacat aaccaaagaa gcgcagatta
      661 gaaaaatggc aatggatttt tgtaaaaact ccaacaacta taaaattaca tatagggggt
      721 aatatgttaa gtaagcattt ttctcgcaag gaatttaaat gtaaatgcgg aaaatgtgat
      781 tatgatacaa tcgacgctga attactggtg attcttgagg atgtacgcga acacttcggg
      841 aaaccagtga ttattaacag tggcaaccgt tgcccgactc ataacaagaa tgtaggcggt
      901 gcaaccaaca gctatcacgt tcgaggtcgt gcggctgaca ttgtggtcaa gggtgtctcc
      961 cctgatattg tccatgctta tcttgatggg aaatatccta ctcaatacgg cttaggcaaa
     1021 tataaaacct ttacgcatat tgattccaga tcaaaaaaat cacggtggaa tggataaaac
     1081 aaaagcgcct ctggttgaag taatgaaaga accggaagac ttagaactcg atttaagccc
     1141 tttagaggct ctttaataca attaaattaa aaaggggtac atctgtaccc cttaactgtt
     1201 ttagctcgtt atacgtcgat ttaggccgct taaattccaa attcctgtaa tggacctggt
     1261 atttcctctt ctggtttcca gttcttatct gtcagcatgt ctaaacatgg atgcaaaaca
     1321 agcccgttac tctccagaat ctgcctatca ttgatagtct tcaaatcaac gaagatccgg
     1381 cctttcctcg ttcctcttga attttctatt acaactctct gggtctttcc atcgatgtat
     1441 tccagtacaa acaagttttt gtttctgtca atcctgctac acccgatccg ctttagagct
     1501 tgcttaatct taaaccggac ctttagtcgt tcctcgttat aaacatcttc taaatcaaat
     1561 tctgcgagct tctcccagcc ttgagagtcc acagaataca tttcctcttc tgccatcttg
     1621 atctgattgt agagtgtttc acgctcgcta ttgagggtag taatctgatc ggctagctcc
     1681 ttcgttgctc ctgtcatagc agaaagggca atcaggttat caatcttgcg tgatatttca
     1741 tcaacctgta ctttcaacgc cggaaccgga ttagccttat cctcagcaat ccagatttta
     1801 tcagccagca attgcaatac agctttctct aattgatccc ctcgaaaact ccagtttgga
     1861 tgctcgcaat caagtctacg tgaacgggtc gcatcacatg tataacgata ctggtcagaa
     1921 cgtttgtttg ttcccttcac tttaaccata gcgcaaccgc aatgatcaca tttcaatagg
     1981 ccaatgccac taagcagggg gatcggttta gcttcttcct tgtctccaaa gttccatgct
     2041 ctagcaccaa tatttttctt aaggtgatag aacgtagcat catcaataac acgagggtaa
     2101 taattgttta actcatagtt aacaccatcg acagagacct ccttgatacc aatcaaagcg
     2161 cgggtatgaa acaacctttc aaccattgcc cgtgaccatt ttgaatgctt cttgttgctt
     2221 actgctggtg gtggtgcata tgtctcgtta aggtgatcca gtatttcggc ggttgaacgt
     2281 ccattcttcc gcaattccac aatctcctga acaacaggga agtacaccgg atgaggcagc
     2341 acataaccgg aagtggtatc cgtccaccac atattctttc caacttcctc gattgccact
     2401 gccggattct ggggattttc ttgatgcgac ttaatcttaa tcagtgcgct tgaattagta
     2461 cgattgcgct tagtctgact ttcctcgtta gccctagcaa atagcatgac actcaagagt
     2521 aaatcgatgg gattagcatt aaccgattca cggctataca ctctgccatc cataccagtg
     2581 acaatagtta ttcccctacg gataatcgag agaaatagct cttgtgcttc aatgatggac
     2641 tggcgagaga tacggtccaa gttttccaca accaaccagc taccaacagg aacggatcga
     2701 ccaatctcat ccagaaagcg ggataatgca cctgttttac tgttagcccc tttgaatgct
     2761 gatacaccaa aatcctgata gcttgtaact agctcaagat catattttgc ggcaatctct
     2821 gcggctgttc tcttctggcg ttcataagaa ctaccatccg cttgcttcat agacgagaag
     2881 cggatatatg aatacaactt agttttcatt ataaccccat agtcaaaagc cccgcgctag
     2941 gcgaggctaa tattcattac agcaagattg cacatcgatt taacatcttt taaaaaaccg
     3001 ctcataattt cggctggtaa tgtttctgta aaaccatctt ccattaactc tcttgaaaca
     3061 tatccggtct tccccttcca gatctgctta attctcgttt ctacatccag cgccttttgc
     3121 ccgtcttcaa atttcataga gaacacaatt tcatgattca gctttgaacg gttgctgtgc
     3181 tgtctcattc gctgttccgg ttttcggttg gtgattccaa atttaatggc ttcaacttcg
     3241 cccgtgacgt gctgtacata tagataacca gctttagcca cgttataacc gtaacttgca
     3301 caacaaggac aacggctacc attatacaga aaccttctaa tagatgccgt cttgaatgta
     3361 tgaccagccg aacaggtaag ggatatttta gtgttatacc ctttccattc tccaacaata
     3421 ccatgat
//
LOCUS       DAPGRN010000233         3403 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPGRN010000233 DAPGRN010000000
VERSION     DAPGRN010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.237"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..199
                     /locus_tag="RHW33_004697"
     CDS             26..199
                     /locus_tag="RHW33_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648108.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            201..545
                     /locus_tag="RHW33_004698"
     CDS             201..545
                     /locus_tag="RHW33_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV1648109.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            549..884
                     /locus_tag="RHW33_004699"
     CDS             549..884
                     /locus_tag="RHW33_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV1648110.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(940..3183)
                     /locus_tag="RHW33_004700"
     CDS             complement(940..3183)
                     /locus_tag="RHW33_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV1648111.1"
                     /translation="MNGDNSVETGGAYSAGLLSQVNDSEKMVNNTRLETTDKTNIVTS
                     GNNAVGVLACSSPGESRTCVDAVDDEVSDSNSYEVISRADLKMNGGSITTKGINSYGA
                     YANGKKAYINLDYVALETVADGSYAVAIRQGNIDIKNSSITTTGTKAPIAKIYNGGEL
                     FFSNVTAVSEQDKGISIDASNIDSQAKIALLSVELSSALDSIDVNKTTTDVSILNRSI
                     ITPGNNVLVNNAGGGLNIISSDSTLNGATKLVSGTTTLKLSENTIWNMKDDSVVTHLT
                     NSGSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRLIVEGNTS
                     GSTTVYVKNAGGSGAATLNGIELITVNGNESPADAFRQGDARIAAGAFEYQLKQQGKN
                     WYLTSYQSVNEEDNSSEGNSESTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGE
                     TRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGADLLTGGFTDSD
                     SWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYSAGLYATWFADDISKKGAYIDAWAQ
                     YSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQPQAQAIW
                     MGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPFVEMNWIH
                     NSKDFAVSMNGVKVEQDGARNLGEIKLGVNGNLNPAASVWGNVGVQLGDNGYNDTAVM
                     VGLKYKF"
BASE COUNT          917 a          789 c          706 g          991 t
ORIGIN      
        1 tcttgaataa aaatattgtc aatcaatgag ttattcctca tttaagatta ttctcattca
       61 cgtaaaaaac ataattccta ttatcacagc cacgctcatt ttgaattatc tgaataatag
      121 cgagcgttct ttagttaagc agatcttaat tgaagacgaa attattgtgt gtgcaactta
      181 cttaataccg gacatctaag ttgaagaaaa aaatacttgc cttcgggctt atctcagcat
      241 tgttctgctc cacgccagcg atggcggata tgaaccgcac cacgaaaggt gcattgctag
      301 gcgcaggcgt tggattactt actggcaatg gtgttaatgg cgtactgaaa ggtgcagctg
      361 ttggcgctgg tgttggtgca gtaacagaaa aaggccgcga cggtaaaaat gcgcgtaaag
      421 gtgcaaaagt gggtgccgcc gttggtgctg tcactggcgt tctgaccggc aatggcctcg
      481 aaggggcgat taaaggtgcc gtgattggcg gtacgggtgg tgcaattctg ggtaaaatga
      541 aataaggaat gatgatgaaa aaatttgctt tactggcggg tctgtttgtt ttcgcaccaa
      601 tgacctgggc gcaggactac aatattaaaa acggtttgcc gtcagaaacc tatattactt
      661 gcgcagaagc caacgaaatg gcaaaaactg acagcgcaca ggttgcagaa attgttgcgg
      721 taatgggtaa tgccagcgtt gccagccgtg atttaaaaat tgagcaatct ccggaactga
      781 gcgccaaagt ggtagaaaaa ctgaatcagg tttgcgctaa agacccgcaa atgctgttga
      841 tcacggcaat tgatgacacc atgcgtgcta tcggcaaaaa ataatattta atatgttacc
      901 gcgtcgatga attcgacgcg gtattttagc taacggggat cagaacttat atttcaggcc
      961 caccatcact gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattacccca
     1021 gacactggcc gccggattca gattgccatt tacgccaagt ttaatttccc ccagattacg
     1081 cgccccgtct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg
     1141 gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc
     1201 ctgagtacga ataaaggtgc ggaaaccaag tcgggtctgg atgttgttat ttgcgtcatt
     1261 ctcaatacgt gagccattgg cttccgtatg cgcattatga tcgacgccca tccagatagc
     1321 ctgtgcctgt ggctggaaga tccacgtata tttcgccgct tccagaccaa aggatttatt
     1381 cagggcaaag ccgtaacccg cttccagcga gacggttgcg cctttcgcgc tataggattc
     1441 ataggctaat tcatcccctt tcaccgagtt tttaaaccag ctatattgcg cccaggcgtc
     1501 aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag gtggcataca gccctgcgct
     1561 atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag ttgagttgta
     1621 gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactgtcgg taaaaccacc
     1681 ggttaacaga tcggccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc
     1741 gttgctgtca cgccaggcat tatgtccgcc aatttgacgt agccaaagtc ggcttgaacg
     1801 ctcctgttca gttacaggat cgatgtaacg cgtttcacct gcacgatcgt tcagacgcat
     1861 aacaaaaaga gtgttagcgg ctgccaggtt agcgacgtaa cttccagctt cggggcgcag
     1921 gacgggggta ggcgtttctg ttgattctga atttccctct gagctgttat cctcctcatt
     1981 cactgactga taacttgtca aataccagtt tttgccttgt tgttttagtt gatattcgaa
     2041 agctccagct gcaatccttg catcaccttg tctgaaggca tctgctggag attcattgcc
     2101 attcacagtt atgagttcga tcccgtttag cgtggccgcg ccgcttcctc cagcattttt
     2161 cacatagacg gtagtagaac ccgaagtatt accctccaca ataagtctat ccgtagccga
     2221 tttatcatca cctaatacag tgcggatatt aagctgacca ttattaccga cataattacc
     2281 ttttacggtt aacgtttttc cctgggtaaa tgtttgacca tcatcatacg aaagattgat
     2341 aatactgcct gaattagtca gatgggtaac aacggagtca tctttcatat tccagattgt
     2401 gttttctgaa agcttcagcg tggttgtgcc gctaacaagt ttagtcgctc catttagagt
     2461 agagtcggac gaaattatgt ttaagccacc tccagcatta ttaaccagaa cattattacc
     2521 aggtgtaata atacttcgat taaggatact tacatccgtt gtagttttgt taacatcaat
     2581 actatccaaa gcacttgaaa gttcaacact taatagtgct attttggctt gagaatcgat
     2641 atttgatgca tcaattgata ttcctttatc ttgttctgat accgcggtga cattggaaaa
     2701 aaataactct ccaccattgt atatttttgc aatgggggct ttagtgcctg ttgttgtaat
     2761 agaactattt tttatatcaa tgttaccttg tcgaatagca actgcataac ttccatcagc
     2821 cacagtttca agtgccacat aatctaaatt aatatatgct tttttcccat tagcataagc
     2881 accatagcta ttaatgcctt tagttgttat ggaaccacca ttcattttta aatcagcacg
     2941 gctaataact tcgtaactgt tagaatcact aacttcatca tctacagcat cgacacatgt
     3001 tcgagactct ccaggacttg aacatgcaag aacacctact gcattattcc cagaggtaac
     3061 aatgttcgtt ttatctgtgg tttcaagacg ggtgttatta accatctttt cagaatcatt
     3121 aacctgactt aaaagtcccg ctgaatatgc cccccccgtc tcgactgaat tatcaccatt
     3181 cataaagatg gtgccaccaa attcactgac tactgcgctg gctgttatcc ctccattttc
     3241 tttgtcagat acacttccag acgtctgaat atcgctatcg cttatatata atgctttgtt
     3301 ttgactatca atagaaatac cataagaacc atgaccaata gtcatcacac tggcattttt
     3361 tatatatcca gttacaccgt catccactat cacagcatgg att
//
LOCUS       DAPGRN010000234         3401 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPGRN010000234 DAPGRN010000000
VERSION     DAPGRN010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.238"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..382
                     /locus_tag="RHW33_004701"
     CDS             176..382
                     /locus_tag="RHW33_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1648112.1"
                     /translation="MYIPQKFLPKSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            408..860
                     /locus_tag="RHW33_004702"
     CDS             408..860
                     /locus_tag="RHW33_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1648113.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRVAGAQTQPEEGQQFSGQPQPQPPEGEDYGFSDDIPF"
     gene            923..1156
                     /locus_tag="RHW33_004703"
     CDS             923..1156
                     /locus_tag="RHW33_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV1648114.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            1222..3180
                     /locus_tag="RHW33_004704"
                     /pseudo
     CDS             1222..3180
                     /locus_tag="RHW33_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3235..>3401
                     /gene="psiB"
                     /locus_tag="RHW33_004705"
     CDS             3235..>3401
                     /gene="psiB"
                     /locus_tag="RHW33_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV1648115.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDVPD
                     NWTMNGEYGSE"
BASE COUNT          793 a          892 c         1074 g          642 t
ORIGIN      
        1 ggcatcccgg caaaaggccg ccgggatgta aggggcaacg ccgtttcgca aaccgcgctt
       61 cacgtcgttg cggcgggtcc ctgcccgttg ttctgtccgg tgccattcac ctgtttaagc
      121 ccctgtcgcc gggggcaaag acagccacaa ccgtctccgc cagtccaggg gtgaaatgta
      181 catcccgcaa aagtttttgc ccaaaagcag gcaaaaactt ttgcggtctg cccccggaca
      241 gtcgtttccg tccgtggcag tgatgcccct tgcggcgaag gttctcaaaa aggtgatggt
      301 gaataccgga gggaacaaaa ggcaggtccc cgcaaaacgt caaaaccgcg gctcccgaaa
      361 ctcaaaagat gatgggggct gaaaacatca acagaaggag gcacatcatg gcagttcgtg
      421 gcattaacaa ggtcattctc gtaggacgcc tgggaaaaga tccggaagtc cgttacatcc
      481 ccaacggggg cgcagtggca aacctgcagg tcgccacgtc ggaaacctgg cgtgacaaac
      541 agacagggga gatgcgggag cagacagaat ggcatcgcgt ggtgctgttc ggcaagctcg
      601 cggaagtggc tggcgaatat ctgcgcaagg gcgcgcaggt ctacatcgaa ggtcagcttc
      661 gcacccgtag ctgggaagat aacggtatca cccgttacgt caccgaaatt cttgttaaga
      721 ccacaggcac catgcagatg ctgggacgtg tcgcaggtgc tcagactcag ccggaagagg
      781 ggcaacagtt cagcggtcag cctcagccgc aaccgccgga gggggaggat tacgggtttt
      841 cagacgatat cccgttctga tcgggctgac tgtgataacc gccccgcccc gttctgtgcg
      901 gggcatcacc ggagagatga gaatgagcga atatttcaga atacttcagg ggctgcctga
      961 cggtcctttt acccgcaaac atgccgaagc tgttgccgca cagtaccgga acgtctttat
     1021 cgaggatgac cacggcgaac agtttcgcct ggttgtccgt aataacggtg caatggtctg
     1081 gcgtacctgg aattttgagg acggtgccgg gtactggatg aaccatgtca tcagggattt
     1141 cgggattctt aagtaagaga ggtgccggac ggggacacgt ccggcagaca atcattatta
     1201 ccgttttggg aggtactaac tatgtcagtt acagagtcta aggcaaaaac ggagcgtaaa
     1261 tccagccgta aacctgcaaa aacgcaggaa acagtcctgt ctaccctgct ggcgcagacg
     1321 gaggaagtga gcgtgccgct ggcctcgctg attaagtcac cgctgaatgt gcgcacggtg
     1381 ccgtattttg cggagtccgt cagcgaactg gcggactcca ttaaaggtgt cggcctgtta
     1441 cagaatctgg ttgttcatgc cctgccaggt gaccgttacg gtgtcgccgc aggtggtcgc
     1501 cgactggcag cactcaacat gctggcagag cgtgacatca ttccggctga ctggcctgtc
     1561 cgtgtgaaaa ttattccgca ggagctggcg acagccgcat caatgactga aaacggtcat
     1621 cgtcgggata tgcaccctgc cgaacagatt gccggcttcc gcgcaatggc gcaggaaggc
     1681 aaaacacctg cacaaatcgg tgatttgctg ggttattcgc cccgccacgt tcagcgaatg
     1741 ctgaaactgg cagaccttgc gcctgtcatc ctcgatgcgc tggcagaaga ccgcatcacc
     1801 accgaacact gtcaggcgct ggcgctggag aacgacaccg cgcgtcaggt gcaggtgttt
     1861 gaagctgcct gtcagtcggg atggggagga aaaccggaag tacagaccat tcgtcgtctg
     1921 gtgaccgaaa gtgaagtggc ggtggcgggg aacggtaaat tccacttcgt gggggctgat
     1981 gccttctcgc cagatgaact gcgcaccgat ttgttcagcg acgacgaggg tggctatgtg
     2041 gactgtgttg cgctcgatgc cgccctgctg gaaaaactcc aggccgtcgc agagttcctt
     2101 cgggaagccg aaggctgggg atggtgcgcc ggacgcatgg aacctgtcgg tgagtgccgt
     2161 gaagatgccc gggcataccg cagtctgccg gagccggaag aggtgctgac ggaggcggaa
     2221 gaagaatgcc tgaacgaact gatggcacgt tacgacgcgc tggaaaacca gtgtgaggaa
     2281 tccgacctgc tggaagcaga aatgaagctg atgcgctgca tagcgaaggt cagagcgtgg
     2341 acgccggaga tgcgtgccgg aagcggtgtg gtggtgtcct ggcgttacgg taatgtgtgt
     2401 gtccagcgtg gtgtgcagtt gcgcagtgag gatgatgcgg ctgacgatgg tgaccgcacg
     2461 gaacaggtgc aggagaaagc gtcagtggag gaaatcagtc tgccactgct gacgaaaatg
     2521 tcctcagagc gcacgctggc agtctaggcg gcgcttatgc aacagccgga caaatctctg
     2581 gcactgctgg catggacgct ctgcctgaat gtgtttggta gcggggcgta cagcaatccc
     2641 gcaaaaattc gtctggaatg tgagcattat gcgctgacca gcgatgcgcc atcggggaag
     2701 gaaggtgccg cattcatggc gctgatggca gaaaaagccc gtcttgcagc cctgttaccg
     2761 gagggatggt cacgggacat gacgacgttc ctgtcactca gccaggaggt gctgttatcc
     2821 ctgctcagtt tctgcactgc gtgcagcatc cacggcgttc agacccgcga gtgtggtcac
     2881 tcgtcacgca gtccgcttga cccgctggaa accgctatcg gattccacat gcgcgactgg
     2941 tggcagccga caaaagcaaa cttcttcgga cacctgaaaa agccgcagat tatcgcagct
     3001 ctgaatgagg ccggcctgtc cggtgctgca cgggacgcgg agaagatgaa gaaaggcgat
     3061 gcggctgaac atgcagagtt ccacatgaaa gacaaccgct gggtgcctgg ctggatgtgt
     3121 gcaccacgcc cacagacgga tgccactgaa cgcaccgata acctggctga tgccgcctga
     3181 tgaaaaacca caccgccccg ccggagacgg ggcggcagca agggagatac cgtgatgaaa
     3241 actgaactga ccctgaatgt cctgcagacc atgaacgcac aggaatatga agatatccgg
     3301 gctgccggaa gtgatgaacg ccgtgaactg actcacgccg tgatgcggga gctggatgtg
     3361 ccggataact ggacgatgaa cggcgagtac ggcagcgagt t
//
LOCUS       DAPGRN010000235         3370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPGRN010000235 DAPGRN010000000
VERSION     DAPGRN010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.239"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..929)
                     /gene="sctW"
                     /locus_tag="RHW33_004706"
     CDS             complement(<1..929)
                     /gene="sctW"
                     /locus_tag="RHW33_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     SctW"
                     /protein_id="HDV1648116.1"
                     /translation="MAIHVEHVGVLERAREVSRLEDIITEDNEDIEAEMPKMGDDPAG
                     KEARFLQATDEMSAALMQFMKKKIYEEQLANFLDGEEYVLEDQPIEKTDKVMEALKAA
                     TTHDYEVYSFAKKLFPDESDLVVVLRAILRKKQISENVRLNAEALLRKVNQETTKKFI
                     NSGINSALKAKLFGQALSLNPKLLRASYRQFLMAEDDAVDTYVEWIGSYGYQNRMLVT
                     KFIKETLFSDINALDASCSSLEFGMFLNKLSQLLSLQSAEALFLKTLMNNPIIKKFIS
                     AEDYWIFFLISLIKFPETAEELLNNALVTLPADA"
     gene            complement(934..2637)
                     /locus_tag="RHW33_004707"
     CDS             complement(934..2637)
                     /locus_tag="RHW33_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HDV1648117.1"
                     /translation="MKIKLRITIILISVLCIFNGLLTPGAYAAAANGYVANKENLRSF
                     FETVSSYAGKPTIVSKLAMKKQISGNFDLTEPYALIERLSAQMGLIWYDDGKAIYIYD
                     SSEMRNALINLRKVSTNEFNNFLKKSGLYNSRYEIKGGGNGTFYVSGPPVYVDLVVNA
                     AKLMEQNSDGIEIGRNKVGIIHLVNTFVNDRTYELRGEKIVIPGMAKVLSTLLNNNIK
                     QSTGVNVLSEISSRQQLKNVSRMPPFPGAEEDDDLQVEKIISTAGAPETDDIQIIAYP
                     DTNSLLVKGTVSQVDFIEKLVATLDIPKRHIELSLWIIDIDKTDLEQLGADWSGTIKI
                     GSSLSASFNNSGSISTLDGTQFIATIQALAQKRRAAVVARPVVLTQENIPAIFDNNRT
                     FYTKLVGERTAELDEVTYGTMISVLPRFAARNQIELLLNIEDGNEINSDKTNVDDLPQ
                     VGRTLISTIARVPQGKSLLIGGYTRDTNTYESRKIPILGSIPFIGKLFGYEGTNANNI
                     VRVFLIEPREIDERMMNNANEAAVDARAITQQMAKNKEINDELLQKWIKTYLNREVVG
                     G"
     gene            complement(2634..3356)
                     /locus_tag="RHW33_004708"
     CDS             complement(2634..3356)
                     /locus_tag="RHW33_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1648118.1"
                     /translation="MNVLLQGQKLTLLDPEMWFLRGNENKDVTLVITIGNNHQKLMVV
                     EDILLLIDRSQIEVTAGKVIYHPLRIDILSKLLAFIDESTGSVEREHHELFADALPFA
                     SEVVLFSKVASEAWFLATYLSSDNINEILFQHLRKTECYKLVRYLLSQSLIQTSLYDL
                     GELYGVSYSHFRRLCSYALGGKVKTELCGWRVARAVLEIIEGNSDMTTIAHKYGYSSS
                     SHFSAEVKSRLGKTPRELCKKL"
BASE COUNT          959 a          734 c          567 g         1110 t
ORIGIN      
        1 gcatctgcgg gaagggtcac cagtgcattg ttcaggagtt cctctgcagt ctcaggaaat
       61 tttattaacg atatcagaaa aaatatccag taatcttctg cgctgataaa ttttttaatt
      121 attgggttat tcattaaagt tttcagaaat aaagcctctg ccgactgaag agacaatagt
      181 tgtgacaact tattgagaaa cataccaaat tccaatgatg aacaactcgc atcaagtgca
      241 tttatatctg agaaaagcgt ctctttgata aattttgtca ccaacattct attttgatag
      301 ccataactac caatccattc gacataagta tctacagcat catcttctgc cattaaaaat
      361 tgccgataac tcgcccttag taattttgga tttaatgaca gcgcctggcc aaataacttt
      421 gcttttaatg ccgagttaat gccgctatta atgaattttt tagtagtctc ttgattaact
      481 tttctaagta atgcttcagc gtttagccgg acattttctg atatttgttt tttacgtaaa
      541 attgcacgta acacaactac cagatcgctt tcatctggga ataatttttt agcaaagcta
      601 tacacttcat agtcatgggt agttgcagcc ttaagcgcct ccataacttt gtcggttttt
      661 tctattggct ggtcttccag gacatattct tctccgtcca gaaaattggc cagttgttct
      721 tcgtagattt ttttcttcat aaactgcatt aacgctgctg acatctcgtc agtagcttgt
      781 aagaaccgag cttctttacc tgcgggatca tcccccattt taggcatttc agcttcaata
      841 tcttcattat cttctgtgat aatgtcctct aagcgagaaa cttccctggc tctttctaat
      901 acaccgacat gctctacatg aatcgccatt tcactatcct ccgacgactt cacgattcag
      961 gtaagttttt atccatttct gcagtaattc atcgttaatt tctttatttt ttgccatttg
     1021 ctgtgtaatc gctctggcat caaccgcagc ctcattcgca ttattcatca tgcgctcatc
     1081 gatctctctt ggctcaataa gaaagacacg aacgatgtta ttcgcatttg ttccttcata
     1141 accaaataat ttaccaataa atggaatgct gcctaatatt gggatcttgc gactttcata
     1201 ggtattcgta tcgcgtgtat atcccccaat caaaagactt ttcccttggg gcacgcgtgc
     1261 aatagtactt ataagtgttc taccaacctg aggcagatca tcaacattgg tcttgtcgga
     1321 attgatttca tttccatctt caatattcaa gagtaattca atttgatttc gcgcagcaaa
     1381 tcgtggtagc acgctaatca tcgtaccata ggttacttca tccaattccg cagtacgctc
     1441 gccgactaat ttagtataga aagtgcggtt attatcaaat atagcaggaa tattctcctg
     1501 tgttaaaacg acgggacgag caacaacggc ggctcttctt ttttgtgcaa gtgcctgtat
     1561 tgttgcaata aactgtgtcc catcaagggt actaattgaa cctgaattat taaatgatgc
     1621 acttaagctg gatccaattt ttatcgtgcc cgaccagtca gcacctagct gttctagatc
     1681 agtcttatca atatctatta tccataaaga taattcaatg tgtcgctttg gaatatcaag
     1741 cgtagctact aacttctcga taaaatcaac ctgagatact gttcctttaa caagtaaact
     1801 gttggtatca ggatatgcaa taatttgtat atcgtcagtc tctggcgctc ctgctgtcga
     1861 tattattttc tctacttgta ggtcatcatc ttcttcagcc ccaggaaaag gaggcatacg
     1921 tgatacgttt tttagttgtt gacgacttga aatttcagaa agtacgttca cccctgtgct
     1981 ttgcttaatg ttattattta acaacgtcga tagtactttt gccatgccag gaataactat
     2041 cttttcgcca cgtaactcat aagtccggtc attaacgaat gtgttgacca gatgaattat
     2101 tcctacttta tttcgaccga tctcaatgcc atcgctgttt tgctccatta atttcgcagc
     2161 attaacgacc aggtcaacat aaaccggtgg ccccgataca tagaaagtac cattgccgcc
     2221 ccctttaatt tcataacgag agttatacag acccgatttt tttaagaaat tattaaactc
     2281 attcgtcgat acctttctca gattaatcaa tgcattacgc atctctgatg agtcgtagat
     2341 ataaatagct ttgccatcat cataccaaat aagccccatc tgtgccgaca ggcgttcaat
     2401 caaggcatag ggttctgtta aatcaaaatt tccgctgatc tgtttcttca tggcaagctt
     2461 actaactatt gtaggcttac ccgcatatga tgaaaccgtt tcaaaaaaac tacgtagatt
     2521 ttctttattt gctacgtatc cattcgctgc tgcggcatat gcaccaggag tcaataatcc
     2581 attaaaaatg cataagactg aaattaatat aatagtaatg cgtaatttta ttttcataac
     2641 tttttacata gttctctcgg tgtttttcct aaccggcttt ttacttcggc tgaaaagtgt
     2701 gatgaggacg agtaaccata tttatgcgca atagttgtca tatcactgtt tccttcaatg
     2761 atttccaaca cggctctggc aactctccag ccacataatt ccgttttaac tttaccaccc
     2821 aatgcataac tacataaacg gcgaaaatgt gaatacgata cgccgtaaag ctcaccaaga
     2881 tcatagagtg aagtctgtat gagcgattgc gataataaat aacgaaccag tttatagcat
     2941 tctgtttttc ttagatgctg aaacaggatt tcattaatat tatcgctgct cagataagtg
     3001 gctaaaaacc aggcttcact tgcaacttta ctgaatagca caacttctga tgcaaagggc
     3061 aaggcatcag caaacaattc atgatgttcc ctttccacac tccccgttga ttcatctata
     3121 aaagctaata atttactcag gatatcaatg cgaagtggat gataaattac tttccctgca
     3181 gttacctcaa tttggcttcg gtcaatgagc agtaatatat cttccactac cattaatttt
     3241 tgatggttat taccaatcgt aataacaagg gttacatcct tgttttcatt tccccgtaag
     3301 aaccacattt cagggtccag tagggttaac ttctgtcctt gaagcaaaac attcattgga
     3361 agtaacagcc
//
LOCUS       DAPGRN010000236         3316 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPGRN010000236 DAPGRN010000000
VERSION     DAPGRN010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.240"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1028)
                     /locus_tag="RHW33_004709"
     CDS             complement(<1..1028)
                     /locus_tag="RHW33_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648119.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMAQHNLA
                     NDLLREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSFGASRHPVSTRINYRNFLPPDDSNSVIRLQTRSAYHNGERSSVRKKRPRLG
                     RLLLSLIEDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVAS"
     gene            1382..1978
                     /locus_tag="RHW33_004710"
     CDS             1382..1978
                     /locus_tag="RHW33_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HDV1648120.1"
                     /translation="MEKNQEERKIPVIVVKKRRTFSLPSLSEKTDIIESAFTEQAAES
                     AHAGINSSAVETHIPEAPARKKKKRRFPRPSHWTREYTHECVEKIKILFPHLRAEGGG
                     FIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDG
                     HPKGQVSASEAQSAGRRLVTLEQKWLRMQAQQENISGQ"
     gene            2034..2537
                     /locus_tag="RHW33_004711"
     CDS             2034..2537
                     /locus_tag="RHW33_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648121.1"
                     /translation="MSKSGAEKIQFILNNHEQAYRRLKPVYTFLELASWISVVLLIIY
                     LSGKNHVLTAAFLCFAWTAFWVLFLLHYFPKVFSILDRQSLLNLMEATKDSPDVKQEL
                     LNRLFSGKRMTGRDEMDICRLLRKNEEEKIRQSELNIIMNFIDEQQLTNSDIPKKSTV
                     PNEDKKA"
     gene            complement(2889..3170)
                     /locus_tag="RHW33_004712"
     CDS             complement(2889..3170)
                     /locus_tag="RHW33_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1648122.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(3167..>3316)
                     /locus_tag="RHW33_004713"
     CDS             complement(3167..>3316)
                     /locus_tag="RHW33_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HDV1648123.1"
                     /translation="VERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSR
                     KVAGK"
BASE COUNT          960 a          780 c          730 g          846 t
ORIGIN      
        1 cagctggcca cattcagaag agcagtgagc gtgtttcgtt ccagtttgct gtccacaggc
       61 accggacatg tcagttgata aagagcatga gcataccctt cactgcagat gattctgcca
      121 tcggcactcc ggcaaaaagc aagaataacc cagtaagggg gacgtattcc gccctgcgct
      181 tcaccattaa tactgatccc ccgcacaggc ctgatgacct gaatattacc gttcgcccag
      241 tgaatttcca cagcatcacg acagatgtgc tgcgccataa aaatctgaaa gaacatatgt
      301 tttcttctgg caggccagtg tcgttctgaa ttctccagcg tttccattaa ccgctcctga
      361 gacctttcag acattcctgg acggaaatgg ataatttcag ataaggatct tccccgaata
      421 tactcctgct gtagtgtaac ctgccgtatg gcatcaagac actcacgata attagtccgg
      481 atacggggat tagccaggga atccagttta ttcagccctg catcttcaat aagcgacagg
      541 agtaaacggc caaggcgcgg acgtttcttg cgaacggagg agcgctcacc attatggtaa
      601 gcagagcggg tctgcagtct gataacagaa ttactgtcat caggtggtaa aaagtttctg
      661 taatttatac gggtgctaac gggatgccgg cttgctccga aggatgtttc ctcttcattt
      721 ccggataact gacgatacat ggggcattca ggtgcatgct catgattaaa actggacaga
      781 taaagagtac ccgtattgtt tttaagagca gcaaaattca tggccggcga atctcccgga
      841 atacagtcac actgtaacca tgcatcatac tcacgcattt cccggagaag atcattggcc
      901 aggttatgtt gtgccatggt tgctggcttt ttcagtccac agaccgtctt tactgcagtc
      961 tcttcctcac tgctaagctc ccgaatcact ttgccccggg gattcttctt tacaagtatc
     1021 attggcataa tgaaagcact ccttatgtac atgcttaaac agtaagtaaa atatacaaag
     1081 aacaccccat gaaacaaagg cagagatgaa ctgtgcgccc cgatatcttc ttctgacatc
     1141 ctttcaaatg aattgttttt atttatatca tgacaacatt aacgtgagag aatatataaa
     1201 ccatgtttcg gtcagggttc agtcgaatat caattcgact gtcacgccat aactgttacc
     1261 acctcgagta taaatattca gagaacggac ttattaacca tgaaaaatca cctgacggaa
     1321 aatttactaa tttatggatt gtgatattat taaagagtaa aaccagccag tcgaggtatt
     1381 tatggaaaaa aatcaggaag aaaggaaaat ccccgtcatt gtagtgaaaa aacgtcgtac
     1441 cttttcgtta ccttccttat ccgaaaaaac agatattatc gaatctgcat ttactgaaca
     1501 ggcagcagaa tcagcccacg ccggaataaa ttcatctgcc gtcgaaacac atatccccga
     1561 agcaccagcc aggaagaaga aaaaacgccg tttcccacgt ccctcccact ggaccagaga
     1621 gtatactcac gagtgcgtgg aaaaaataaa gatcctgttt cctcatcttc gtgctgaagg
     1681 tggtggattt attcccctga aaattggcat aaacaatgat atttcagcat ttctggcaga
     1741 acacccggag acagaactga ccatggacga atggctctgt gcagtgtcct gcatcacctc
     1801 acgccgtgta tatctgcagc ggacagctgt cgccggcgtc ccacgttatg gtctggatgg
     1861 tcacccaaaa gggcaggtct cagcatctga agcacagagt gcaggtcgca gactggtgac
     1921 attagaacaa aagtggctca ggatgcaggc acagcaggag aatatttctg ggcaataaac
     1981 actcctgata acagctggac attacgtatc agaaaaatgg gaagagaatt attatgagta
     2041 aatcaggtgc agaaaaaatt caattcatcc tgaataatca tgaacaggca tacagacgcc
     2101 tgaagcctgt atatactttt ttagaactgg catcctggat ttcagttgtt ctgctgatta
     2161 tatacctatc aggcaagaat cacgtgctta cagcagcttt tctctgcttt gcatggacag
     2221 cattctgggt tcttttcctt ttacattatt tcccaaaagt tttctctatt ctcgatcgtc
     2281 agagtctgct caacctcatg gaggccacca aagactcacc agatgtaaag caggagctgc
     2341 ttaaccgcct tttttccgga aaaagaatga ccgggcggga tgaaatggat atctgccgtc
     2401 ttctgagaaa aaatgaagaa gaaaaaatcc gtcagagtga actgaatatc atcatgaatt
     2461 ttattgatga acagcagtta acgaatagtg atattccgaa gaaaagtacg gtcccaaatg
     2521 aagataaaaa ggcataagat gatgatatga aattaataac caggcaagca gaggatggca
     2581 tggtttcatc ctaagattga taggggttaa cctgtcagat aaaaggactg agtttacaca
     2641 acgtatcttg ttgacgcgca aaatttcggg ttcggtcatg aaaaagtaac atattctatg
     2701 ctggaacaga gcaattttgc attctccgtc caggagtttg aggggaaagt agtcagtgtt
     2761 ttacggtcag tcagggaaag tgtgcaatga taaataacac gaaaggaatc gtaggaggta
     2821 acttaacaat aactaagctt cttagcgtac gttttcgttc cattggccct caaaccccga
     2881 cagcccctct atggccactt ctgtgcagta tgcaatacgc gcaggatata cacttttccc
     2941 cactggctgt cgacctcata aaccagaaca aaatgcgggt gaataaccag ttcccgggta
     3001 ccttccacac gaccgttgcg gcctttatac ggctgcccgg ccaggctgct ggctgaataa
     3061 ccaatccgtt cgtccagttc aatggcggcc atcaggttct gctccgcaat gtactcacga
     3121 atgcgcttcc tgtcctggct ggccagcatg gtccagaata tctccatcat ttaccggcga
     3181 ccttcctgct catttcatcc cgccatgctg cagcagagga ttcgaccatg tcatggggaa
     3241 gcacatcgcc gtgctcgagt tgctgacgtc ctgccgcaac ctggtcgcgg aaccaggtat
     3301 tatgctgccg ttcaac
//
LOCUS       DAPGRN010000237         3268 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPGRN010000237 DAPGRN010000000
VERSION     DAPGRN010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.241"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(94..621)
                     /gene="mobB"
                     /locus_tag="RHW33_004714"
     CDS             complement(94..621)
                     /gene="mobB"
                     /locus_tag="RHW33_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV1648124.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQNQDG"
     gene            complement(603..1187)
                     /gene="mobA"
                     /locus_tag="RHW33_004715"
     CDS             complement(603..1187)
                     /gene="mobA"
                     /locus_tag="RHW33_004715"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV1648125.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1257..1526
                     /locus_tag="RHW33_004716"
     CDS             1257..1526
                     /locus_tag="RHW33_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV1648126.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1603..2589
                     /gene="srkA"
                     /locus_tag="RHW33_004717"
     CDS             1603..2589
                     /gene="srkA"
                     /locus_tag="RHW33_004717"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV1648127.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2606..3232
                     /gene="dsbA"
                     /locus_tag="RHW33_004718"
     CDS             2606..3232
                     /gene="dsbA"
                     /locus_tag="RHW33_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV1648128.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKR"
BASE COUNT          831 a          792 c          798 g          847 t
ORIGIN      
        1 aagcgaaagg ccatcctgac ggatggcctt tttgcgttgg tgcaaaaaaa tgccggatgc
       61 gacgctggcg tgtctgctcc agcctactct tgcttatccg tcctgatttt gcatccactc
      121 caccacaaaa tcagccagcc cctcaacatc attaatatcc agtaacgcga catcaagatt
      181 aagcggcaca tcactggcta cagcaataac atgcctgtct atcactaatt cttcaggtcg
      241 atgtccggct ccatcgcgaa acagcacaat ctttgcgatc tcttcatgct taaacccttc
      301 gaccagaatc aaatccagct ttgaggtatc cattcgactt gcgagaaaat gtagatccag
      361 ctcttcttcg tctggtgttt ccgtcatcaa ggcccatcgt tgctggctgg caacgatggt
      421 ttgtgccgcg ccagccttgc gcagctcata gctatctttg cctggcttat caacatccat
      481 atcatggtgc gtatgcttaa tcagccctgg acggatccct ctggcgcata atgccgggat
      541 cagttttttc aaaagcgtag tttttccggt accactccac gcggcaaagg cgagtaacgg
      601 tatcatcgtt tttcctgcca tcgggcaagc tcctctggcg tattcacgtt aacaaatgca
      661 tctttatgat cgctgaaatc aaccgcatga ccgccagcca gacgcataaa tgccattacc
      721 cggcgttctc ctgcttgcag atattctagt aataaaggct caatagcgcg gtttaccaga
      781 gcaatagtcg ggtgatcgcg ttcaccgtca tggacccaca cgacaggcgc atctttgcgc
      841 tgatgattaa gccgggcggc taaatcatgg ggaatgtaag gcgtatcgca cggacaaaac
      901 aaaaaccact caccagcttc ctgctgcatt actgaaagca tccctgccag agggcctggg
      961 taatccgcca gtgaatcttc aatcactttc agaccgcctg cctgatagat ttcctgatga
     1021 cgattagcat taaccacgac gtgagagagc tgcgtcataa gcgcgtcagc gacatgttgc
     1081 cacagcggtt tgccatttaa gtccagcaat cctttatcta cgccgcccat tcgtctggct
     1141 ttaccgcctg ccagcacaac gcctgttatc gtcgtcatca gattcaccga tatcgcctct
     1201 tttattgtgg gattgaccct gctaacgtgt ctgtctcaag agtaaggagc atcactatga
     1261 aatgtaaacg tctgaatgaa gttattgaac tcctccagcc agcctggcaa aaagagccag
     1321 accttaacct gctgcaattt ttgcagaaac tggcgaaaga gtcaggtttt gacggcgaac
     1381 tggcggattt gacggatgac attctgatct atcacctgaa aatgcgtgat tccgcgaaag
     1441 atgcggtgat cccgggtttg cagaaagatt atgaagaaga cttcaaaacg gcgctgttac
     1501 gcgctcgcgg cgtaattaaa gagtaaaagc ttgtaagcgg cgccaccaaa atcatcgtga
     1561 aatgatatcc ttcatcattc gtaatgtttt ccggatgatg ggatgaacaa cagcgctttt
     1621 actttccaga cactacaccc ggataccatc atggacgctc tgtttgagca agggatccgg
     1681 gtggattccg gtcttacccc gcttaacagc tatgaaaacc gtgtctatca atttcaggac
     1741 gaagatcgtc gacgttttgt cgtcaaattt tatcgccctg aacgttggac agccgatcaa
     1801 atcctcgaag aacatcaatt tgcgttgcag ctggtaaatg atgaagttcc ggtcgcagca
     1861 cctgtggcct ttaacggtca gactttattg aatcatcagg gattttattt cgctgttttt
     1921 cccagcgtcg gtggtcgcca gttcgaagct gataatatcg atcagatgga agcggttggg
     1981 cgttatttag ggcgtatgca ccagacgggg cgcaaacagc tttttatcca tcgcccgacc
     2041 atcggtctga atgaatatct cattgagcca cgcaagctgt ttgaggacgc tacactgata
     2101 ccttccgggt tgaaagcggc attcctgaaa gcgacagatg agctgatcgc cgccgttaca
     2161 gcacactggc gggaagattt caccgttctg cggctacatg gagactgcca cgccgggaat
     2221 attctctggc gcgatggtcc aatgtttgtt gatctggatg atgcacgtaa tggtccagct
     2281 attcaggatt tgtggatgtt gctcaatggc gataaagccg agcagcggat gcaactggaa
     2341 actattattg aagcttatga agaatttagc gagttcgaca ccgctgaaat cggactgatt
     2401 gaacctttac gcgccatgcg tttggtttat tatcttgcct ggctaatgcg gcgttgggct
     2461 gatcccgcgt tcccgaaaaa tttcccgtgg ttaaccgggg aagattactg gctacgacag
     2521 acggcgactt ttatagaaca ggcaaaagtt ctacaagaac cccctttgca attaacacct
     2581 atgtattaat cggagagagt agatcatgaa aaagatttgg ctggcgctgg ctggtttagt
     2641 tttagcgttt agcgcatcgg cggcgcagta tgaagatggt aaacagtaca ctaccctgga
     2701 aaaaccggta gctggcgcgc cgcaagtgct ggagtttttc tctttcttct gcccgcactg
     2761 ctatcagttt gaagaagttc tgcatatttc tgataatgtg aagaaaaaac tgccggaagg
     2821 cgtgaagatg actaaatacc acgtcaactt catgggtggt gacctgggca aagatctgac
     2881 tcaggcatgg gctgtggcga tggcgctggg cgtggaagac aaagtgactg ttccgctgtt
     2941 tgaaggcgta cagaaaaccc agaccattcg ttctgcttct gatatccgcg atgtatttat
     3001 caacgcaggt attaaaggtg aagagtacga cgcggcgtgg aacagcttcg tggtgaaatc
     3061 tctggtcgct cagcaggaaa aagctgcagc tgacgtgcaa ttgcgtggtg ttccggcgat
     3121 gtttgttaac ggtaaatatc agctgaatcc gcagggtatg gataccagca atatggatgt
     3181 ttttgttcag cagtatgctg atacagtgaa atatctgtcc gagaaaagat aattcaatgt
     3241 aaattacata aagcccgtga atattcac
//
LOCUS       DAPGRN010000238         2987 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPGRN010000238 DAPGRN010000000
VERSION     DAPGRN010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.242"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..886
                     /locus_tag="RHW33_004719"
     CDS             <1..886
                     /locus_tag="RHW33_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDV1648129.1"
                     /translation="IARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTD
                     VLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAV
                     PPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGF
                     NSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVR
                     SPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPRE
                     YLRQRACNGLSDNRCLEI"
     gene            1316..1792
                     /locus_tag="RHW33_004720"
     CDS             1316..1792
                     /locus_tag="RHW33_004720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648130.1"
                     /translation="MKFERIYKNITGLSCPIFGIQWKAPIIEADEAKKIVIFLEDKRV
                     LFNPVDMEGASHCTSSVINIRSELTKVLQGLPSDSHLAKQLRKMRKACQEFCDSVGSP
                     NFIQLDHPVQISILKRALFKLRQKCGNSLAEIAVTYGLDVDDELAIIIPFNNPSNI"
     gene            2316..>2987
                     /locus_tag="RHW33_004721"
     CDS             2316..>2987
                     /locus_tag="RHW33_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV1648131.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGYSVASVATRLDITTHSLYSWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDILKKAALDVRLPEQFSIIGKLRAH
                     YPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMA
                     TRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVW
                     CGDVTY"
BASE COUNT          802 a          685 c          753 g          747 t
ORIGIN      
        1 tattgcccgg ggatggggag taagcttagt cagccgttgt ctccgggtgt cgcgtgcgca
       61 gttgcacgtc attctcagac gaaccgatga ctggatggat ggccgccgca gtcgtcacac
      121 tgatgatacg gatgtgcttc tccgtataca ccatgttatc ggggagctgc ccacgtatgg
      181 ttatcgtcgg gtatgggcgc tgcttcgcag acaggcagaa cttgatggta tgcctgcgat
      241 caatgccaaa cgtgtttacc ggatcatgcg ccagaatgcg ctgttgcttg agcgaaaacc
      301 tgctgtaccg ccatcgaaac gggcacatac aggcagagtg gccgtgaaag aaagcaatca
      361 gcgatggtgc tctgacgggt tcgagttctg ctgtgataac ggagagagac tgcgtgtcac
      421 gttcgcgctg gactgctgtg atcgtgaggc actgcactgg gcggtcacta ccggcggctt
      481 caacagtgaa acagtacagg acgtcatgct gggagcggtg gaacgccgct tcggcaacga
      541 tcttccgtcg tctccagtgg agtggctgac ggataatggt tcatgctacc gggctaatga
      601 aacacgccag ttcgcccgga tgttgggact tgaaccgaag aacacggcgg tgcggagtcc
      661 ggagagtaac ggaatagcag agagcttcgt gaaaacgata aagcgtgact acatcagtat
      721 catgcccaaa ccagacgggt taacggcagc aaagaacctt gcagaggcgt tcgagcatta
      781 taacgaatgg catccgcata gtgcgctggg ttatcgctcg ccacgggaat atctgcggca
      841 gcgggcttgt aatgggttaa gtgataacag atgtctggaa atataggggc aaatccaaca
      901 ccccactctt tgcaacatcg agtataagtc gaaaaacgtt tggttgttct ggttatttgc
      961 atttactgcc attcatagaa atgaacagat cgattgatag gactcaaatg ttatagcatc
     1021 tctcttctca tagtctgctt tgtgccagga gcggacgcta acctgattga cgggaaatca
     1081 tattaagtct gcataaaaaa ttagaatatc tttagttact attactaatg tgtgtataac
     1141 ttatgcctaa tttatcgctc cggctgaggg aatcttgcct ccgctgtcac ttttaggcat
     1201 gataacggct atttttacag tggttccgag ttatacgatc taacagatta cttgaaccga
     1261 gtccgtttat cagtatagcg ttttatatgc tcagagttca tccaaggagt gacttttgaa
     1321 atttgaacga atctataaaa atattactgg ccttagctgt cccatattcg ggattcagtg
     1381 gaaggcgcca ataatagaag ctgacgaagc gaagaagatc gtcatttttc ttgaggacaa
     1441 gcgcgttctt ttcaatcctg tcgacatgga gggggctagt cattgcactt cgagtgttat
     1501 caacattaga tctgaattga ctaaagtcct acaaggtttg ccgagcgatt cacatttagc
     1561 gaaacaatta aggaaaatga gaaaagcatg ccaagaattc tgtgacagcg ttggctcccc
     1621 taactttatc caacttgatc atcctgttca aatatccata cttaagcgag ccctttttaa
     1681 actgcgacaa aagtgtggta actctctggc agaaatagcg gttacctatg gtcttgatgt
     1741 tgatgacgaa ttagccatta ttatcccttt taataatccc agtaacatat agcaatgact
     1801 atgaatttgc ttgcttgatt aattatcatg caatcgatag ttttggggcg gaagcggtta
     1861 gcaaattagt cattagtgag ccgttatgtc cgttcatcgc tctcagcgga ccttcagctc
     1921 agtgatatcg tccgctctgt gccaggagcg gactgtccaa cccatccctg aagagcctag
     1981 attatgtatg gtaattcagt catcaaaata acgttggtca ccatcacaaa attcacatcc
     2041 tcggatatgg ctcagttcag gaacgctatc gctcaggtga ccgcaacaac cacatacatg
     2101 aagctcggtg taatagatga aacactgagt gcaaagatag ccgtctccaa atttacatac
     2161 gctttcagga gtcatgcaat tggtacatcc agcaggaaat acggagagta gctgttcatc
     2221 cacactgtgg aacaactcct catccagcag tttgaatcgc cacaggttta acagacacct
     2281 cagagtcatt taagatgact taaagagagg tgcccatgag cggtaagcgt tatcccgaag
     2341 agtttaaaat tgaagcagtc aaacaggttg ttgatcgcgg ttattctgtt gccagcgttg
     2401 caacacgtct cgatatcacc acccacagcc tttactcctg gataaagaag tacggtccgg
     2461 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
     2521 agctgaagcg ggttaccgac gaacgggaca tattaaaaaa agccgctctt gatgtcagac
     2581 tccctgaaca gttctcgata atcgggaaac tcagagcgca ttatcctgtg gtcacactct
     2641 gccatgtgtt cggggttcat cgcagcagct acagatactg gaaaaaccgt cctgaaaaac
     2701 cagacggcag acgggctgta ttacgcagtc aggtacttga gctacatggc atcagccacg
     2761 gttcggccgg agcaagaagc atcgccacaa tggcaacccg gagaggctac cagatgggac
     2821 gctggcttgc tggcaggctc atgaaagagc tggggctggt cagctgtcag cagccgactc
     2881 accggtataa acgtggtggt catgagcacg ttgctatccc taactacctt gaacggcagt
     2941 tcgccgtgac cgagccaaat caggtgtggt gcggtgatgt gacctat
//
LOCUS       DAPGRN010000239         2860 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPGRN010000239 DAPGRN010000000
VERSION     DAPGRN010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.243"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..586
                     /locus_tag="RHW33_004722"
     CDS             <1..586
                     /locus_tag="RHW33_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly protein PilX"
                     /protein_id="HDV1648132.1"
                     /translation="TGNNKKNEPHDKGWAILEQGTIALVVLFVIVVVLGSLYALRTRA
                     NVATETANIQTIITSAQSLLKGSDGYTFTSSAKMTGALIQMGVIPSGMTVQGDKTSGT
                     ATLYNAWGGAVTVAPASTSGFNNGFTVTYDKVPQDACIQIATRISKTGLTNGITLNST
                     AHSDGKVTTEEASTQCKADNGSTGTNKLIFTING"
     gene            596..1156
                     /locus_tag="RHW33_004723"
     CDS             596..1156
                     /locus_tag="RHW33_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1648133.1"
                     /translation="MSGGTVPLRLISLILLFSLIPPARASYVAPKQIWTNKWDNCFAA
                     AGARYQIEPLLLKAISAGESSLRPGAININKDKKTGKASSTDYGLMQINSTHIPKLIN
                     MGVIKKSEDLITKPCLNIHIGSWILARHFQICGVSWNCLGSYNAGFRKDRHETREQYA
                     NKIWRIYRDMKGICLPGQGGRQCRQS"
     gene            1141..1797
                     /locus_tag="RHW33_004724"
     CDS             1141..1797
                     /locus_tag="RHW33_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV1648134.1"
                     /translation="MQTIVGISLSPVYILPLMFTFSIIGRTLLFRVRYFLSDTGHLWY
                     KTHPAVLSGIWLYSIAVALIILSSSPLLYRIHAVLILSFILQMAVTDALTGLLPGTFT
                     RRFLIAGMLSQITTDIWWFRTTEFATAAIVLFCLHKLVNRHRLNIGTGDLWLIAGITA
                     WSGLYNAIWCVLLGTGGFVLWHSTWCIKGHKEGPLGPWLCFGHVLLLLDNLYQPLWVI
                     "
     gene            1797..>2860
                     /gene="pilV"
                     /locus_tag="RHW33_004725"
     CDS             1797..>2860
                     /gene="pilV"
                     /locus_tag="RHW33_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HDV1648135.1"
                     /translation="MKKYDRGWASLETGAALLIVMLLIAWGAGIWQDYIQTKGWQTEA
                     RLVSNWTSAARSYIGKNYTTLQGSSTTTTPAVITTTMLKNTGFLSSGFTETNSEGQRL
                     QAYVVRNAQNPELLQAMVVSSGGTPYPVKALIQMAKDITTGLGGYIQDGKTATGALRS
                     WSVALSNYGAKSGNGHIAVLLSTDELSGAAEDTDRLYRFQVNGRPDLNKMHTAIDMGS
                     NNLNNVGAVNAQTGNFSGNVNGVNGTFSGQVKGNSGNFDVNVTAGGDIRSNNGWLITR
                     NSKGWLNETHGGGFYMSDGSWVRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLERT
                     AVAGASCSPNGLVGRDNTGA"
BASE COUNT          819 a          607 c          681 g          753 t
ORIGIN      
        1 aactggaaat aataagaaga atgagcctca tgataaagga tgggcaattt tggagcaggg
       61 aacaatagcg ctcgttgttc tttttgttat agtcgttgtt ctgggtagtt tgtatgcgtt
      121 gcgtacaaga gccaatgtgg caactgaaac cgcaaatata caaacaatta tcacaagtgc
      181 ccagagcctg ctaaaaggta gcgatggata tacatttacc agcagtgcca aaatgacagg
      241 tgccctcatt cagatgggtg tcattccctc tgggatgacc gtacagggcg acaaaacatc
      301 tggtacagca acgctctata acgcatgggg cggtgctgta actgttgccc cggcttcaac
      361 atcagggttc aacaacggtt ttaccgttac ctatgataaa gtcccacagg atgcctgtat
      421 ccagattgcc acacgaatca gtaaaactgg tttaactaac ggcattaccc tgaacagtac
      481 tgctcacagt gatggaaaag tcactactga agaagccagt acgcaatgca aggcagataa
      541 tggcagcact ggtacaaaca aacttatctt caccatcaat ggttaaggag ttttaatgtc
      601 agggggtaca gtacccctca gacttatttc attaatcctg cttttttctc ttattccacc
      661 cgcccgagct tcatacgtag ctcccaaaca gatctggacc aacaagtggg acaattgctt
      721 tgccgctgcc ggcgcgcgct atcaaattga accattatta ttgaaggcta tttctgccgg
      781 ggaatcttcg ttgaggccgg gcgcaattaa tataaataag gacaaaaaaa caggtaaagc
      841 cagtagcacg gattatgggt tgatgcaaat aaactcaacc catattccaa agctgataaa
      901 tatgggcgta ataaaaaagt ctgaggattt aattaccaaa ccatgcctta atatccatat
      961 aggaagctgg atactggcaa gacattttca gatatgtggc gtcagctgga attgccttgg
     1021 gtcatacaat gccggattcc gtaaggacag acatgaaaca agagagcaat atgccaataa
     1081 aatatggaga atttatcgtg acatgaaagg gatatgtctt cccggacagg gaggcaggca
     1141 atgcagacaa tcgtaggcat aagtctatcc cctgtttata tactgccatt gatgttcaca
     1201 ttttccatta ttggacgaac tctgctattc agagttcgtt attttttatc tgatacggga
     1261 catctctggt ataagacaca ccctgctgtt ctgtcaggaa tatggctata cagtatagct
     1321 gtggcgttaa ttattttatc atcatcacca ctgctctacc gtattcatgc tgtccttatt
     1381 ctttcgttca tactgcaaat ggcggtaacg gatgcactga caggtttatt accaggaaca
     1441 ttcacccgac gttttttaat tgcaggaatg ctatcacaaa tcacgactga tatatggtgg
     1501 ttccgtacaa cagaatttgc cactgctgca attgtcttat tctgccttca taaattagtt
     1561 aaccgccatc gtcttaatat tggcactgga gacctgtggc ttattgcagg tattaccgca
     1621 tggtcagggt tatataacgc catatggtgt gtgttgctgg gcacaggagg atttgtctta
     1681 tggcattcaa catggtgcat aaaagggcat aaagaaggcc ctctgggtcc atggctatgt
     1741 tttggccatg tccttttgct gctggataat ctttatcaac cactatgggt aatctgatga
     1801 aaaaatatga ccgaggctgg gcatcgctgg aaactggcgc ggctttactg attgttatgc
     1861 tcctgattgc ctggggagcg ggcatatggc aggactatat tcaaacaaaa ggatggcaaa
     1921 ctgaagcacg tcttgtcagc aactggacca gtgcggcccg ctcttatata gggaaaaact
     1981 acacgactct ccagggcagt agtaccacaa caactcctgc cgttatcacg acaaccatgc
     2041 tgaaaaatac cggctttttg tccagcgggt ttactgagac aaacagcgag gggcagcggt
     2101 tacaggcata tgtggttcga aacgcccaaa acccggaatt actacaggca atggttgtat
     2161 ccagtggtgg cacaccttat ccagtgaaag cacttatcca gatggctaag gatattacca
     2221 ctggtcttgg tggatatatc caggacggca aaacagccac aggtgcatta cgttcctggt
     2281 cagtagcttt aagtaattat ggtgccaaaa gcggtaacgg gcatattgcc gtattgttat
     2341 cgacagatga acttagtggt gcagctgagg acactgatcg tctttacaga ttccaggtca
     2401 atggtcgccc tgacttaaac aaaatgcaca cggccattga tatgggatca aataacctga
     2461 ataacgttgg ggcagtaaat gcccagacag gtaatttcag cggcaatgtg aatggtgtaa
     2521 atggcacttt cagcggtcag gtaaaaggca atagcggaaa ctttgacgta aatgtcaccg
     2581 ctggcggtga tatcagaagt aataatggtt ggttaattac tcgtaacagt aaaggctggc
     2641 tcaatgaaac tcacggtggc ggattttata tgtccgatgg atcatgggtt cgaagtgtaa
     2701 acaacaaggg catctatacc ggcggtcagg tgaaaggcgg tactgttcgg gctgatggtc
     2761 gcctttatac tggtgaatac ttacaactgg aaagaactgc cgttgctggc gcatcatgtt
     2821 cgcctaacgg ccttgtaggc cgcgataata caggggcaat
//
LOCUS       DAPGRN010000240         2807 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPGRN010000240 DAPGRN010000000
VERSION     DAPGRN010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.244"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..2422
                     /gene="ydeP"
                     /locus_tag="RHW33_004726"
     CDS             143..2422
                     /gene="ydeP"
                     /locus_tag="RHW33_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV1648136.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2670..>2807
                     /gene="safA"
                     /locus_tag="RHW33_004727"
     CDS             2670..>2807
                     /gene="safA"
                     /locus_tag="RHW33_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV1648137.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     IS"
BASE COUNT          753 a          656 c          682 g          716 t
ORIGIN      
        1 ttgtttaacg ataatagatt aaaaatagaa agagagtagt cccctactct tatatatcca
       61 tgttggcgat aatccctttg tatgtactgt gcatcatcgc tattacaaat cctaataatt
      121 catttccaca caggataagt agatgaagaa aaaaattgaa tcctaccagg gtgctgccgg
      181 tggttggggt gctgttaaat ccgtagcgaa tgcagtacgt aagcagatgg atatacgcca
      241 ggatgttatt gccatgtttg acatgaataa gccagagggc tttgactgtc cgggttgtgc
      301 atggccagat cctaagcaca gtgcgtcatt cgacatttgt gaaaacggcg caaaagcaat
      361 cgcctgggaa gtcacggata agcaggtaaa cgcctctttc tttgctgaga atacggttca
      421 atcattactt acctggggag accacgagct tgaggctgcg gggcgactca ctcagccttt
      481 gaaatatgat gccgtcagcg actgttacaa gccattaagc tggcaacaag ctttcgatga
      541 aattggcgca cgccttcaaa gctatagtga tcccaatcag gttgaattct atacttcggg
      601 ccgcacttcc aatgaagctg cctttcttta tcagcttttt gcccgtgaat acgggagcaa
      661 taactttccc gactgctcca acatgtgcca tgaaccgaca agcgtgggtt tggcagcgag
      721 tatcggtgta ggtaaaggga ccgtgttgct ggaagacttt gagaagtgcg atttagtcat
      781 ttgcattggg cataaccctg gtacaaacca ccctcgcatg ctgacttcgt tgcgcgcttt
      841 agtgaaacgg ggagcgaaaa tgatcgccat caatcctcta caggaacgtg gcctggagcg
      901 atttaccgca ccgcaaaacc cgtttgaaat gctgacgaac tctgagactc agttggccag
      961 tgcctactac aacgtgcgca ttggtggcga tatggcgttg ctcaagggga tgatgcgcct
     1021 gttaattgag cgcgatgatg ctgcaagtgc cgcaggtcgg ccctcattgc ttgatgacga
     1081 atttattcaa acgcataccg tcggctttga cgagctacgc cgtgacgttc tcaattccga
     1141 gtggaaagat atcgaacgta tttctggact aagtcagaca caaatcgccg aactggctga
     1201 tgcatatgcc gctgccgaac gcaccattat ctgttacgga atggggatca ctcagcacga
     1261 acatggtacc cagaatgtac agcaactggt caatctgctg ttgatgaaag gtaacattgg
     1321 caagcctggt gcgggtatct gcccactacg tggccactct aatgtacagg gcgaccgaac
     1381 cgtcggtatc accgagaaac cgtctgcaga gtttctggct cgcctgggtg agcgctatgg
     1441 cttcacacca cctcaagcac ctggacatgc tgcaattgcc agcatgcaag caatatgtac
     1501 agggcaggct cgagcattga tctgcatggg gggcaacttt gcactggcaa tgccagatcg
     1561 ggaagcgagc gctgtaccgt taacgcaatt agatctggcg gtacacgtag ccactaagct
     1621 taaccgctct catctgctga ccgcacggca tagctatatt ctgccggttc tgggacgtag
     1681 cgagattgac atgcaaaaaa gcggtgcgca ggcggtaacc gttgaggatt caatgtcgat
     1741 gattcatgcc tcgcgtggcg tgttaaaacc cgccggtgta atgctgaaat cagagtgtgc
     1801 agtggtcgcg ggaatcgcgc aggcagcact accccagagc gtggtagcct gggagtatct
     1861 ggtggaagat tatgatcgca ttcgcaatga cattgaagct gtgctgccag agttcgccga
     1921 ctataaccaa cgcatccgtc atcccggtgg ttttcacctg ataaatgcag ctgctgaaag
     1981 gcgctggatg acgccgtcag gtaaggctaa tttcattacc agcaaagggc tgttagaaga
     2041 tccctcttca gcgtttaaca gtaagctggt catggcgaca gtacgcagcc acgatcagta
     2101 caacacgacg atttatggta tggatgatcg ctatcgaggg gtattcggtc aacgagatgt
     2161 ggtctttatg agtgctaaac aagctaaaat ttgccgtgta aaaaacggcg aaagagttaa
     2221 tcttattgcg cttacgccag acggtaagcg cagctcacgc cgcatggata gattaaaagt
     2281 ggtcatttac cctatggctg accgctcact ggtgacctat tttccagaat cgaatcacat
     2341 gctaacactt gataaccacg atccattaag tggcattcct ggctataaaa gtattccggt
     2401 tgaattagaa ccatcaaatt aatgcctctt ctcatttctt ctgctgtcat ccgcacagca
     2461 gaagaattcc tcattgacta ttatcgcgca atttgctcac atggattaaa ttaaactaca
     2521 tactataaga tataaacttc tgcctacagc tgtaagaaac tccgctcagt actgaagcac
     2581 cagtcctatt tcctcttttc tccagcctgt tatattaagc atactgatta atgattttta
     2641 acgttatccg ctaaataaac atatttgaaa tgcatgcgac cacagtgaaa aacaaaatca
     2701 cgcaaagaga caactataaa gagatcatgt ctgtaattgt ggttgtctta ttactgaccc
     2761 ttacgttgat agccattttt tcggcaattg atcagctggg catctca
//
LOCUS       DAPGRN010000241         2789 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPGRN010000241 DAPGRN010000000
VERSION     DAPGRN010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.245"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            216..>2789
                     /locus_tag="RHW33_004728"
     CDS             216..>2789
                     /locus_tag="RHW33_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648138.1"
                     /translation="MFFSRRSLSGADGRALYAYRCTDTEYESLAELLRTYAPRSYPRT
                     IFISYSDVLFSIYAAEFIRRTHTVGHPKWDTILDSINWKVPYVHRQKLVNDGIRYWKR
                     KIRNLGQASGYLHTLACEGGLPIRMIENESGYLITYFRRIYQALRGQSSQYPAAKIAQ
                     ELGDTIPVTMQNELVYEIAGEFCETLCRLLSEHPPHSSDPVSALRKLSPDWHLQLPLV
                     LPEANAAEIVRRLLSQSSEIRSASSLQVERIWVDVDDSWYCDARFRFPATMRTEQLTS
                     LFECHIQPEQTRLIISGKWKNGGARLAMLSRYEQQDWRVELLPIAMQKLSGADAMAEI
                     SLSLHEGPILLGHTIPKGGYELTEELPWVFEAMNESESQLKLVGMGSVSSRLNALFIS
                     LPKNSHLDISGEGEFDIPRLLKNSERSLTKISGVFSVVLHDGAVCTIRTQQLYDSAIE
                     YYIKSTEVELVKSDYPVHRAWPKIGWKKDLQYGIVPEKELFWRSIRSGNNAWYSVASE
                     MPKGQIEVRRIVNDEVLFSGKVVVLPADFDINIIPESAQQGIIMLSGITDTRIDKYSN
                     NEKVTLKSDYSQNECAIYYNSSLMLENTVDLRVSWKDGSNLKLLLPKPVSGGRFVTND
                     GSVHFDGVASIAHLHGIDAELLTISCAGRGYLNIELLDENPVAEKFRYLHADLPLLSG
                     RNDKLQQISLYENYNLLNAMLACAWNSNSTLCVDFYSDRFGKDKATLNIKRYDGSFIE
                     HDQGLLVDIKNSVVFPANRIDELVVDAISLKNPGLHISLLKKDEFAYDLSALNVQDSP
                     WLIVGKLDGTARIAPVIKWMLPVLQTNDLLLNALCEADPEQRKKNFNELIFEIDNNPL
                     QN"
BASE COUNT          828 a          505 c          603 g          853 t
ORIGIN      
        1 aaaataaaca aaacacacat aatttatatg gttaagttcg gtgtagcgct catgattgta
       61 acaagttttt tcgagatata aatgatttgt ctcattttta gcgagttcat cgggttgagc
      121 ttttttatga tgatagtaac ctgttacgaa ttgtatcaaa ggaaggcagg tttcttgtga
      181 aaactccagt taacccactt ttgcaatggc ttaacatgtt ttttagtcgc cgttcattat
      241 ccggagctga tggacgggcg ttatatgctt accgttgtac tgatacagag tacgaaagtt
      301 tggcagaact actgcgtaca tatgcccccc gtagttatcc aagaacgata ttcatttcct
      361 atagcgatgt tctatttagc atatatgccg cagagtttat ccgccggact catacggtcg
      421 gacaccctaa atgggacaca attttagatt ctattaactg gaaagttccg tatgttcatc
      481 gacagaagct ggttaatgat ggcattcgct actggaaaag aaagataaga aacctgggac
      541 aagcttcggg ttatctgcat acactggctt gtgaaggtgg tttgcctatc cgcatgattg
      601 aaaatgagag cggttatttg attacctatt tcagaaggat atatcaggcg cttcgtgggc
      661 aatcatctca atatcctgcc gctaaaattg cacaagaatt aggcgatacg attcctgtca
      721 caatgcaaaa cgaactggtc tatgaaatag ccggtgaatt ctgcgagact ctctgccgat
      781 tacttagtga gcatcctcct catagtagtg atcccgtttc tgcattacgt aagctctctc
      841 cagactggca tcttcaactt ccactcgttc tccccgaagc gaatgctgca gagattgtaa
      901 ggcgattact ttctcaatct tctgaaatac gcagtgcaag tagtcttcag gttgaaagaa
      961 tctgggtcga tgttgatgac agttggtatt gcgatgcccg gttccgattc ccggccacaa
     1021 tgcgtacaga acagttaacc tctttgtttg aatgccatat tcagccggag cagacccgac
     1081 tcatcatttc aggaaaatgg aaaaatggcg gggcaaggtt ggcaatgcta agccgttatg
     1141 agcagcaaga ttggcgggtc gagttattac ctattgcgat gcaaaaactc tctggtgcag
     1201 atgcaatggc cgaaatctca ttgtcgcttc atgaaggtcc aattctgtta ggtcacacaa
     1261 ttccaaaagg cggttatgaa cttactgaag aacttccctg ggtttttgaa gcgatgaatg
     1321 agagtgaatc gcagctaaaa cttgttggta tggggtctgt gagttccagg ctaaatgctc
     1381 tgtttatttc gctaccgaaa aatagccatt tagatattag tggagaaggt gagtttgata
     1441 tcccaaggtt gctgaaaaac agtgaacgca gcttaactaa aataagtgga gtatttagcg
     1501 ttgttttaca tgatggtgct gtttgtacaa ttcgcaccca gcaactttat gattctgcga
     1561 ttgaatatta tattaaatcg acagaagttg aactggttaa atcggattat cctgtccatc
     1621 gagcctggcc gaagatcggt tggaaaaagg atctgcaata tggcattgta ccggagaaag
     1681 aacttttttg gcgttccatt cgttcaggta ataatgcctg gtattctgtc gcatcagaaa
     1741 tgccaaaagg acagatagaa gtccggcgaa tagttaatga tgaggtttta tttagcggta
     1801 aagttgtagt tttaccagca gacttcgata ttaatattat acctgagagt gctcagcaag
     1861 gcattattat gctttctggt ataacggata ctagaattga taaatattca aacaatgaaa
     1921 aagtaacact taagtccgat tattcacaaa acgagtgtgc tatttattat aattcatcgc
     1981 tgatgctgga aaataccgtt gatttacggg tctcctggaa agatggttct aatcttaaac
     2041 tattattacc taagccggtt agcggtggcc gatttgtaac taatgatggt tctgttcatt
     2101 ttgatggtgt ggcatctata gcacatttgc atggaataga tgctgagtta ttaaccatat
     2161 catgtgctgg aagaggatat cttaatatcg agttattgga tgaaaatcca gtagctgaaa
     2221 aattccgcta tttacatgcc gaccttcctc ttttatcagg acgcaatgac aaattacaac
     2281 aaatttcact ttatgagaac tataatttgc taaatgctat gctggcatgt gcatggaaca
     2341 gcaatagtac attatgtgtt gatttttact ctgacagatt tggaaaggat aaagcaacac
     2401 tcaatattaa acgctacgat ggtagtttta ttgaacacga tcaagggtta ctggttgata
     2461 taaaaaattc tgttgttttt cctgcaaata gaatagacga actggttgtt gatgctattt
     2521 ctcttaagaa tcctggctta cacatatcat tgttaaaaaa agatgagttt gcttatgatc
     2581 tttcagctct gaatgttcag gatagtccat ggttaattgt gggaaaactt gatggtacag
     2641 ctcgcattgc accagtaatt aaatggatgc tacctgtatt gcagacaaat gatttattac
     2701 taaatgctct atgcgaagca gacccagaac agcgcaaaaa aaattttaat gagctaattt
     2761 ttgaaataga taacaaccca ttgcaaaat
//
LOCUS       DAPGRN010000242         2624 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPGRN010000242 DAPGRN010000000
VERSION     DAPGRN010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.246"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1635)
                     /gene="traD"
                     /locus_tag="RHW33_004729"
     CDS             complement(<1..1635)
                     /gene="traD"
                     /locus_tag="RHW33_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDV1648139.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFINGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHEKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTC"
     gene            complement(1686..2423)
                     /locus_tag="RHW33_004730"
     CDS             complement(1686..2423)
                     /locus_tag="RHW33_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648140.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNSLKPEAVSC
                     NLLSLCVLAPVFFTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSRVSPDSGRFYEPDTVLDHRERVRLFVQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRAAGGYHHRHTGYTWSVSVN"
BASE COUNT          687 a          687 c          626 g          624 t
ORIGIN      
        1 acaggtcaga tccggcagag actgaatgtc ggaataactg accagcgtct ggcgctccat
       61 atccttaccg gtcgataccc cgtcacgtac cgggtcagca ccgtaggaat actgctcgct
      121 ggctttcagg tgctctttct caccaatttc accggcagcg aactctgcaa tcttatggct
      181 gggagaacgg aaaaaggcac gggtgttcat gacgtcaaac agcgtggccg ccgctttctc
      241 accgtagata tcttccagct gggcatagga ctggatacca aacacataac agccaccgaa
      301 cttacgggct tccggcagga tctccaccag gtccggcagt ttgtgtaacg tgggtaactc
      361 gtcacagaaa aaccacacac gacgattacg gttttctccc attgccagca gaccacgaat
      421 ggcaatggac agccacatgg agatcaccgg cttcagggag gcatgggtgt cggcattcga
      481 cgagataaac agccagccgt ttttctgatc ttcccggaca ccacgcatcc agtcacggat
      541 ggtaaaagac tcaccgttat gctcaatccc ctgcaggtaa cggatggctt tcacgtagtt
      601 ggtcagcaca gcacggatgg aaatcgccgt tttctcaatt ttctcttcca ccaggttggc
      661 tgccggtgaa ttacgcagga aggtacgcag tttttcgatt ttgatggaaa gcagtgtgtc
      721 caccagtttg ctgtagctgc ggttggggtc attacgcatc aggtacgccg cttccgcaaa
      781 aatggtacgt cctgacccct gccagaacgg gtcctctttt gtacccatag ggatcagcgt
      841 gttcgcggta ttatcaaaat ccggctgtgt caggcactcc ttccacaaat cccatgcggc
      901 acagcgggca tccagcggat tcaggatttt atcgatggag ggatcatagt aacttttaac
      961 aaattcccct gaacggtcat aaatcaccac catatcaccg cgctgacggg cgtagttggc
     1021 cagacgacgg ataacctccg acttaccggc cccgactgtg ccgtgcagac agaagttctg
     1081 gatttcggaa tcccggataa tcggcaggtc gccaatccgg atatcggagt ccttgccgtc
     1141 ttttttcagc atccgggcaa catctttcgg attgtctgtc agctgacgac cacctgtgac
     1201 ttcattttcg ctctgttgtt taccctgacg ccccagaatc caggaaacaa caaagaaggt
     1261 aatcaggcaa ataaccaggg ccacgacaga cgccagaacg aatgcagacc atagctgctc
     1321 tccgcaccag atcatatatt tttcatgtag tacctgagcc gcgttcatcc ggaaggtttt
     1381 gccgtaatac tggatctcat agaccggctg ggattttatt aaatcccgca tgccttccag
     1441 cgtggtgcac caccagtaaa tacagccgtt gataaacgtc tgccagctta tttttaccca
     1501 taagaccaga ccaacgagga tccagaaaaa aataaacagg cagtaaagca tgatattggc
     1561 gatctggctg aacatgcgga tacgcatgga cgcaatctga ccgccctggg tcatatcctt
     1621 tgcgttaaaa ctcatgatga aactccggga aaatctggaa cagggcagaa aaggatgatg
     1681 tggggttaat tcacagaaac agaccaggtg taccctgtat gccggtggtg atacccgcct
     1741 gcagccctgc cggaagagac cttctctgta acaatgtcag tcaggcgcaa tacttcagac
     1801 cagtcactcc catgcggaaa ctggatctga acaaaaaggc gtacccgctc acggtgatcc
     1861 agaactgtgt ccggttcata aaaacggcca ctgtcaggag aaacacggga cgttttattg
     1921 atgagatcat acttatcggc aggagactgc gatgaaatcg cccaggtgat attctccgcc
     1981 tggtggccat caatacgact gctccccggc gtccatgcaa tttcctgcgc actcgccacg
     2041 atgatctccc tggaaacttc agccagcttt ctgggtgtaa agaataccgg tgccagcaca
     2101 cacagggaca acaaattaca gctgaccgct tccggtttaa gcgaattgct cccggatttt
     2161 tcaggaaaag atatttttgt cagtatttca gttaattcct tatgcctgag agaattaata
     2221 aagtacatga taagcatcat gagaaacaat ataacaataa gccctccgat aaccactaaa
     2281 aggtcagtat caatattatt gctgtacaga tagtctttag tggtcactgt attatcctgt
     2341 atcaccgggg ggtattcagt tacaggcttc tcagtaaatt gattattctc accaacatat
     2401 tcagcgatat tgtctgatgt cataatgact ttctccggaa gatcccggct tcgccggcaa
     2461 cacttcacgg tcgcacaaag aatttgcaca accgctcagt gttttcatat ttatttcgga
     2521 aacgtcggga agtgacaaaa tttattttgt tacgtcccgg ggttggacaa cgagcaacgc
     2581 gaggcgtcag caaggaggcc ggtcagacca gcctcctgac gagg
//
LOCUS       DAPGRN010000243         2622 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPGRN010000243 DAPGRN010000000
VERSION     DAPGRN010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.247"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            101..1531
                     /locus_tag="RHW33_004731"
     CDS             101..1531
                     /locus_tag="RHW33_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV1648141.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1572..2504)
                     /locus_tag="RHW33_004732"
     CDS             complement(1572..2504)
                     /locus_tag="RHW33_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV1648142.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGVVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
BASE COUNT          825 a          546 c          566 g          685 t
ORIGIN      
        1 aataaatata aatacattct gataatgcat cctgccgctg gacattattt cagcataagt
       61 ggatattcaa agttttgctg ttttatcagg gaatatttat atgatacgta agtcagctac
      121 aggtgttatt gttgcgttag ccgtaatctg gggtggtggc acatggtaca caggtacgca
      181 aattcagcct ggtgtcgaaa aatttattaa agattttaac gatgctaaaa agaaaggtga
      241 acatgcctac gatatgacgt taagttatca aaattttgac aaaggctttt ttaattcccg
      301 ttttcaaatg caaatgacat tcgataacgg tgcacctgat ctcaatatca agccaggcca
      361 gaaagttgta tttgatgtgg atgttgagca cggtccgttg cccatcacaa tgttaatgca
      421 tggtaatgtc atcccagcac tggcagcggc aaaagtgaac ttagtgaata atgaactgac
      481 acaaccgcta tttatcgccg cgaaaaataa atcgcccgtg gaagcgacat tgcgattcgc
      541 gtttggtggc tcattctcca cgacattaga tgttgcccct gcagagtatg gaaaattctc
      601 ttttggtgaa ggccagttta cttttaatgg tgatggtagt tcattgtcta acctggatat
      661 tgaaggcaaa gtcgaagata ttgttctgca attttcacca atgaacaaag taacggcaaa
      721 aagttttacc attgattctc tggcgcgatt agaagaaaag aaatttccgg ttggtgaaag
      781 cgagtcgaaa tttaatcaga ttaacattat caatcacggg gaagacgttg cccaaatcga
      841 tgctttcgtt gcaaaaacca ggctggatcg cgttaaagac aaagattata tcaatgtcaa
      901 tctgacctac gaacttgata agttaacaaa agggaatcag caactcggta gtggtgagtg
      961 gtcattgatt gctgaatcta ttgatccctc agcagtgcgc caatttatca tccagtataa
     1021 cattgcgatg cagaagcagc ttgctgcaca ccctgaatta gcaaacgatg aagttgctct
     1081 gcaagaagtg aatgctgcat tgttcaaaga gtatttaccg ttattacaac aaagtgagcc
     1141 gaccattaaa caaccggtaa gatggaagaa cgcactcggc gaactaaatg ccaatctgga
     1201 tatcagtatt gccgacccag ccaaatcttc atcatccaca aacaaagata tcaaatcgct
     1261 caattttgat gtgaagttac cgcttaatgt cgtcacagaa accgcaaaac agcttaattt
     1321 atctgaagga atggatgcgg aaaaagcgca aaagcaggct gataaacaaa tcagcgggat
     1381 gatgacatta ggtcagatgt ttcagttaat cacgattgac aacaataccg cctcgctgca
     1441 gctgcgttat acaccgggta aagttgtttt taacggacag gagatgagcg aagaagaatt
     1501 tatgtctcgt gccggacgtt ttgttcatta aaatactcac tcgcctctgc tcattcagag
     1561 gcgagcaaaa tttacttgtc ctgccgctgt gatgacatca acgacgtcag aagtcggtct
     1621 ttttcctgcc acaatgagtt cagccattgc tgaaaatggc gcttaaagct tttgtcattg
     1681 atgtagtcgc catgtaactc atcggcaata ggctgtaaat ccacatgtac gacaatccgc
     1741 gtcagtttac cgcttaacat atcgaagaac ggctgacggt tattgtccgg ataacacaac
     1801 gtaacattaa gcagtttatc gaattgttta cccagtacat ttagcgccat cgcaatgcct
     1861 gccgcctttg gtggcaacaa gttttggaaa gtggagtggg tttgctgatg tttttcttgc
     1921 gtgaaacggg agccttcaac gaaattgaca atggtggtgg gatgcaggcg aaacttttca
     1981 caagaacgcc gagtagtttc aacatcttta ccacgtcgct ccggatgacg taataaataa
     2041 gcgcgggaat aacgcttcat aaacggcata tccagcgccc agcacgctag gccaagaaat
     2101 ggcacccagg ccagctgttg cttgaggaaa tatttattca tcggaatgtg cttacgaaac
     2161 agcacgcaca gtacgacaat atctgcccaa ctacggtggt tacaaataag cagataccag
     2221 ttcttcttac ttaacccttc cagcccgtga acttcccatt gcaggtgtgg gttcagatgc
     2281 agtaataccg ccagaccttc acaccagcaa tacatcataa aatcacaaaa acgcgaaacc
     2341 tttcgccaaa tgactggcac aggtagcaat agttttacaa ccccggcaat gatgatcggg
     2401 acggaacaaa aaatagtgac caaaatggtc aatacgatac tcagcaaaag ggttatcgca
     2461 gcgagtattc tcgtcataat gaatttattc aaaaggttag ccatagacag tgcgctaaag
     2521 aagcgcaagg cgcagatttt agcagaaaac gacccaaata acggatgatc cttaaggaga
     2581 aaaataattc atatctatcc acattagaaa aaatcccatt at
//
LOCUS       DAPGRN010000244         2520 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPGRN010000244 DAPGRN010000000
VERSION     DAPGRN010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.249"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..127)
                     /locus_tag="RHW33_004733"
     CDS             complement(<1..127)
                     /locus_tag="RHW33_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV1648143.1"
                     /translation="MEHISVIPDYRQAWKVEHKLSDILLLTICAVISGAEGWEDIE"
     gene            complement(351..1651)
                     /gene="ltrA"
                     /locus_tag="RHW33_004734"
                     /pseudo
     CDS             complement(351..1651)
                     /gene="ltrA"
                     /locus_tag="RHW33_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100279.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          585 a          696 c          459 g          780 t
ORIGIN      
        1 cctctatatc ttcccaacct tctgcaccag aaataacggc acaaatagtc aacagtagaa
       61 tatccgataa tttatgttcc actttccagg cttgtctgta atcggggata acagaaatat
      121 gttccatcaa ttttttaatt ccattttgtt ctccttaatt atgtaaggag tatttgatca
      181 tgtataagca ataaaaaaca gctttaggta atgaggaata tctcaatttt taaacataaa
      241 atgcgaatta tttagtacaa aaaagcagcg ttaagtgagt aggccaccgg aacctccccg
      301 acagcctctc gcagaaccgt acgtgaaact ctcgcttcat acggctcccg tcaggcaaac
      361 gcgctttccg gtcctgcatt ccagtgggca aacaactccg gcttctcttt tgacagcctt
      421 tccattagct gacacgcctt cgtctttcta taccgcaggg ttttatattt ctttctcgcc
      481 cacgcaatca gcgtcagatt aatatatctt tgtggtattt cgcgtaataa ttcagccacc
      541 ccgatatcat cggattcagc cttttcgcaa tatcttcgag actcatctcc accctgcgtc
      601 tgagccgcag tttcctgagt ttttcgatca tgcctttctg cgcactgtta ctcaccgccg
      661 gactgaaact cgtaaagaga tttccacttc gctgattacg tgctcttctc gccctgaacg
      721 tataacccag aaaatcaaac tgcactgttt cgtggttcgc ctgtcgttag ctgtctttgc
      781 agtaaactat tttcgttttc tccggatgta attccagtcc acattcctga aaacgttttg
      841 ccagtttatt cagtaattcc tctgcctgtt gtttactgcc acaatgaatt aatccatcat
      901 ctgcataacg acaccaggga atttccggat aatgtaacga cagccattta tcaaacacat
      961 agtgcagaaa taaattcgcc agtaccggac tgatgactcc accctgtggc gtacctttat
     1021 ttctttcctt cagtgttcca tccggcatct gcaccggcgc tgtcagccac ctgcgaatat
     1081 acagtcttac ccatttatcc gcgcaatgtt tatccactgc cctgagtaat aaatcgtggg
     1141 ggatattatc gaataaccct ttaatatcaa actcaagaca ccagtcgtat tgccagcaac
     1201 gttttctggt aactgctatt gcatccagcg ctgattttcc atgccggtat ccgtatgaat
     1261 cattcaggaa ataacgctcc actaatggtt caaacgttac cagcaccgtc atctgcgcta
     1321 ttcgatcgct tactgtcggt attcctaaaa gccttttact gccatccttc ttcggaattt
     1381 ccaccgcacg cactggcggc ggcatccagc ttcccgacga taacctgttc cagattttgt
     1441 acagatttcc tttcagatcc cgctcaaaat ctttcagcgt ctggttatcc actccggcag
     1501 ctccggcatt cgcctttacc cgacggtacg cttccaccac atgcaattta ctgacctgaa
     1561 acggttttgt ctgttcaccc gcagcctcct cctgtttaca gttgggctgt gatgcagact
     1621 gcccgacgtt gccccttcgc tccacattca ttacaaacgc ttcctcacta ctacgaacag
     1681 ctctgccccg gtttcgcaca tcgatactct catactcata atttcggtta tttgtatttc
     1741 tctcttgaca ttgcgacgac tggttcccgt agttcctgca aagagcctga tacagcgtca
     1801 tgcctgctat acgccggaca ccatttacgc agcattcgga attcacccgt aaattaatcc
     1861 aggcggtatc acgaaacctc cggttttgat gtcacttagg actaacgacg tttcaacaca
     1921 ggttcgggtt tactcatctc actgtatctc acacaggatc agatctcgtc tgatcgttcc
     1981 ccctgcgctc actaccttgg ctctttacca cagcagccgg ggttgtttgg gaccgacttc
     2041 cgacagcaga tcccggtggg cctgccacca tctcttcaca agcttatgct tattacatgc
     2101 aaaacgtccc gttttgcatc tttgtgccta cggcacaccg tgatctttcc ctggtccctg
     2161 tcccggattg tagtaccggt gccagttgta gtgcagttcg gattcgtcgt catactgcta
     2221 gcccggatgg cggagcagtt gctctagtca ctcagaatat tgtaatagtg atagagtgca
     2281 gtgccggttt caatatccta acgctggtct tttgcctgta ttaactgatt tagctggttt
     2341 gctacatatt aatgccttca gattttccct ttccttgtct gtgatactgt cgcaaaccct
     2401 actagcccat tctacttcac tcttctccag gcattctatt ttcttattgc tcattgttct
     2461 aatgtttggt gttgcatctc catttaataa catccatgtt tcatttttct tcataaaaca
//
LOCUS       DAPGRN010000245         2445 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPGRN010000245 DAPGRN010000000
VERSION     DAPGRN010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.250"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..854
                     /locus_tag="RHW33_004735"
     CDS             6..854
                     /locus_tag="RHW33_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDV1648144.1"
                     /translation="MNTVTTMNTAVKTASVSLVVDNTKPLTMSTLQIAKLTGKSHDNV
                     LRDFRKMIADIKKGSQLVGGDLIKNEEIGFYTESTYKDPQNRKQPMYEIGEDLCNTLV
                     MGYDAPLRFKVAKEWRLMKEGNQKPTIVEDLNDPAYIAEMFERGARLAREKQQLIDLH
                     IRRAIDAHSLTRLLGEKRGSTKVQRILKGLCEAKHIERRLDASGKPCGYDVLPPAYMY
                     ARQSAHGQLEFTVDVLSVLVELGLIEEETVATLKLPQPNNARAIVNKNAEMIRKNVGS
                     LAVFGI"
     gene            907..1443
                     /locus_tag="RHW33_004736"
     CDS             907..1443
                     /locus_tag="RHW33_004736"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648145.1"
                     /translation="MKMTHKKKFILDLFDCTKTDPSDVGGWPLTADEVYYLMCDSYPD
                     SPYYEPEYSKRYTLTNSIRRTMDSLVADGLLIRTTISKMDGKYYSNRKTMIAYDIPFE
                     LRKGRNPFDEKKYGIYGSLCIGLHRMEEDELKKSSVIEGEFETVVNDDSITLYEAERI
                     YYSQQERHTADLCDMFGI"
     gene            complement(1587..1814)
                     /locus_tag="RHW33_004737"
     CDS             complement(1587..1814)
                     /locus_tag="RHW33_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648146.1"
                     /translation="MTTKATRGRPARFTREQMADIAYAYYTADRGKESKEQILSEHGI
                     SVAQFYKNMKKLDIKFYVQIDDGQIVEATGF"
     gene            complement(1849..>2445)
                     /locus_tag="RHW33_004738"
     CDS             complement(1849..>2445)
                     /locus_tag="RHW33_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648147.1"
                     /translation="NQQERDELELALSCCTDHEYRLPVKHHHKKTDYTTSGFSREEAK
                     EILIEFYRDNGYTDVHNFFKKHRTSHTEFRRVRDWFDFDIKRYYRVDDGPIYRLQWTP
                     IREVLKQKRLNHAITRYRNEAFKKGYGDTRELFVELANVRYSHYYNDPKGFFEVLRKV
                     DISRGTYYSRLRKYGIKAEFFMSVDDSPMFKIKCKSSK"
BASE COUNT          720 a          496 c          506 g          723 t
ORIGIN      
        1 ctactatgaa tactgttact actatgaata ctgctgttaa aactgcttcc gtgtctctgg
       61 ttgttgataa caccaaacca ctgaccatgt ccactttaca gatcgcgaaa ttgaccggaa
      121 agtctcacga taacgttttg cgtgacttcc gaaaaatgat tgctgatatc aaaaaaggta
      181 gtcaactggt cggaggagat ctcatcaaaa atgaggagat tggtttttat acagaatcga
      241 cttataaaga tccacaaaac cgtaagcaac caatgtacga aatcggggaa gacctctgta
      301 atactctggt tatgggatat gatgcaccgc ttcgattcaa agtcgctaaa gaatggcgct
      361 tgatgaaaga aggtaatcaa aaacctacta tcgttgaaga cctgaacgat cccgcttaca
      421 tcgcggaaat gtttgaacgg ggcgctcgtt tagctcgtga aaaacaacaa ttaattgatc
      481 tccatatccg ccgtgctatt gatgcccaca gcttaacccg cttattgggc gaaaaacgag
      541 gctctacgaa ggtacaacgt attcttaagg gattgtgtga ggccaaacac attgagcgcc
      601 gtctggatgc ttctggtaag ccttgtggct atgatgttct tcctcctgct tatatgtacg
      661 ctcgacaatc agcacatggt cagcttgagt ttactgtaga tgtgctttct gtattggttg
      721 aattgggctt gattgaggaa gagacggtag caacattgaa attaccgcaa ccgaataatg
      781 cgcgagctat cgttaataag aatgcggaaa tgattcgtaa gaatgttgga tcacttgctg
      841 tttttggtat ctaatttttt attttttgcc cctgaaatat ggggctttta ttgaggatag
      901 aacattatga aaatgacaca caaaaagaaa ttcattctgg atttgtttga ttgcactaaa
      961 accgatccgt cagatgttgg tggatggcct ttaactgctg atgaagttta ttatttgatg
     1021 tgtgattcgt atcccgatag cccatattac gaaccggaat actctaaacg ttacacatta
     1081 acaaattcga ttcgtcgaac gatggattct cttgtcgctg atggtttact gattagaaca
     1141 actattagta agatggatgg taagtattat tcaaaccgta aaacgatgat tgcgtatgat
     1201 atcccgtttg aattgagaaa aggccgtaat ccatttgatg agaagaaata cggtatatat
     1261 ggtagcctct gtatcggctt acatcgcatg gaagaggacg aattgaaaaa aagtagcgta
     1321 atagaagggg agtttgaaac cgtcgttaat gatgattcta tcacgttata tgaggccgaa
     1381 agaatttact attcacagca agaacgacac acagccgacc tatgtgacat gttcggaatc
     1441 tgattagaag ccctgccgta atggtggggc tttttactat ccaactccca caaattgtgg
     1501 atgtttcccg actccctcaa tttgaggtga tcggaaaatc aatgacttag atacaaaaaa
     1561 gccactccga agagtggcaa agagaatcaa aaacctgttg cttctacgat ctgaccgtca
     1621 tcgatctgaa catagaattt aatgtctaac ttcttcatat tcttatagaa ttgggctact
     1681 gaaatcccat gttcactaag aatctgttct ttcgattcct tcccgcgatc cgctgtatag
     1741 taagcgtagg caatatcagc catctgttca cgggtaaaac gggcagggcg accgcgagta
     1801 gctttagtag tcatagtagt ttctccaaat taaagtgaat gttcaccttt atttagagga
     1861 cttacatttt atcttgaaca tgggtgagtc gtcaaccgac ataaagaact ctgccttaat
     1921 cccgtacttc ctcagccgtg aataatatgt accccgactt atatcaacct tgcgaagcac
     1981 ttcaaagaac cctttaggat cgttgtaata gtggctataa cgcacgttag cgagttcaac
     2041 gaatagttcc cttgtatcac catagccttt cttgaaagcc tcgttacgat agcgtgtaat
     2101 tgcatgattc aatctcttct gtttcaacac ttccctgatc ggtgtccact gcaaccgata
     2161 gatcggaccg tcatccactc gataataacg cttgatatca aaatcaaacc aatctcggac
     2221 tctccgaaac tctgtatgtg atgttctatg cttcttaaag aagttgtgaa cgtctgtata
     2281 accattatcg cggtaaaatt ctattaaaat ctctttcgct tcctcacggc taaaaccgga
     2341 tgttgtgtag tcagtcttct tgtgatggtg cttaactggc aaacgatatt catgatcggt
     2401 acaacaagac aaggctaatt ctaattcatc tctttcttgc tgatt
//
LOCUS       DAPGRN010000246         2423 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPGRN010000246 DAPGRN010000000
VERSION     DAPGRN010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.251"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..533
                     /locus_tag="RHW33_004739"
     CDS             <1..533
                     /locus_tag="RHW33_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HDV1648148.1"
                     /translation="ILRSVNQHSPLLMQLHAAGIRTGDAERILSSGEYWQRQKTLLTE
                     REVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRPRSRQWWQLFRMVSQWHVDVV
                     IVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGE
                     WLNTTGADQQSPEHRS"
     gene            773..1027
                     /gene="repA"
                     /locus_tag="RHW33_004740"
     CDS             773..1027
                     /gene="repA"
                     /locus_tag="RHW33_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDV1648149.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            1263..1337
                     /gene="tap"
                     /locus_tag="RHW33_004741"
     CDS             1263..1337
                     /gene="tap"
                     /locus_tag="RHW33_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDV1648150.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1330..2187
                     /gene="repA"
                     /locus_tag="RHW33_004742"
     CDS             1330..2187
                     /gene="repA"
                     /locus_tag="RHW33_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV1648151.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          623 a          590 c          649 g          561 t
ORIGIN      
        1 ttatcctcag aagtgtaaat cagcattctc ctttgctgat gcaattgcat gcggcaggta
       61 tacggaccgg tgatgcagag cgaatactgt ccagcggtga atactggcaa cgtcagaaga
      121 cgctgctgac agaaagggaa gtcagtttta tgaaaggact gttcagaatt gtggatatga
      181 agcggtggta tctgtgtccg caggtacggg tcgcggatat cgtccagctg aacgggaata
      241 tccggccacg atcacgccag tggtggcagt tattcagaat ggtgtctcag tggcatgttg
      301 atgtggttat cgttgagcgg cgttcgttca gtattgttgc agcagtagag ctggatgatg
      361 ccagccattt acgaccggaa cgcagacgcc gggatattct tctggaagag gttctgaggc
      421 aggctggtat tccgttgctc agaagccacg atgccagaaa actgctgcag atgaccggag
      481 aatggctgaa tacaacaggg gctgatcagc agtccccgga acatcgtagc tgacgccttc
      541 gcgttgctca gttgtccaac cccggaaacg ggaaaaagca agttttcccc gctcccggcg
      601 tttccataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
      661 ccgttgattt gtgcgccaac acagatcttc gtcacaattc tcaagtcgct gatttcaaaa
      721 aactgtagta tcctctgcga aacgatccct gtttaagtat tgaggaggcg agatgtcgca
      781 gacagaaaat gcagtgactt cctcatctgg cgcaaaacga gcatacagaa aggggaatcc
      841 gctttctgat gcagagaaac aaagattatc agtggcccgt aaaagagctt cgttcaagga
      901 agtaaaagta tttcttgaac caaagtataa ggccatgctc atgcaaatgt gtcatgaaga
      961 tggcctgact caggctgaag ttctgaccgc actgataaaa agtgaagcgc aaaaacgatg
     1021 tatgtgatga tgggcttaca ttcttgagtg ttcagaagat tagtgctaga ttactgatcg
     1081 tttaaggaat tttgtggctg gccacgccgt aaggtggcag ggaactggtt ctgatgtgga
     1141 tttacaggag ccagaaaagc aaaaaccccg ataatctttc tatgtttggc gacgaagaag
     1201 attaccgggg ctaacaagaa accgtatagc caacaattca gctatgcggg gagtatagtc
     1261 atatgcccgg aaaagttcaa gacttctttc tgtgctcgct ccttctgcgc attgtaagtg
     1321 caggatggtg tgactgatct tcaacaaacg tattaccgcc aggtaaagaa cccgaatccg
     1381 gtgtttacac cccgtaaagg tgccggaacg ctgaagttct gcgaaaaact gatggaaaag
     1441 gcggtgggct tcacctcccg ttttgatttc gccattcatg tggcgcatgc ccgttcgcgt
     1501 ggtctgcgtc gacgcatgcc gccggtactg cgtcgacggg ccattgatgc gctgctgcag
     1561 gggctgtgtt tccactatga cccgctggcc aaccgcgtcc agtgctccat caccacactg
     1621 gccattgagt gcggactggc gacggagtct gctgccggaa aactctccat cacccgtgcc
     1681 acccgggccc tgacgttcct gtcagagctg ggactgatta cctaccagac ggaatatgac
     1741 ccgcttatcg ggtgctacat tccgaccgac atcacgttca caccggctct gtttgctgcc
     1801 cttgatgtgt ctgaggatgc agtggcagct gcgcgccgca gtcgtgttga atgggaaaac
     1861 agacagcgca aaaagcaggg gctggatacc ctgggtatgg atgagctgat agcgaaagcc
     1921 tggcgttttg tgcgtgagcg tttccgcagt taccagacag agcttaagtc ccgtggaata
     1981 aaacgtgccc gtgcgcgtcg tgatgcggac agggaacgtc aggatattgt caccctggtg
     2041 aaacggcagc tgacgcgcga aatcgcggaa gggcgcttca ctgctaatcg tgaggcagta
     2101 aaacgcgaag tggagcgtcg tgtgaaggag cgcatgattc tgtcacgtaa ccgtaattac
     2161 agccggctgg ccacagcttc cccctgaaag tgacctcctc agaataatcc ggcccgcgcc
     2221 ggaggcattc gcacgcctga agtctgtcag cgaacaaaaa agcagcacta catacaaaaa
     2281 acaatctcac catccacctt ctggagcatc cggttccccc tgtttttaat acaaaatgcg
     2341 cctcagcgac ggggaatttt gcttatccac atttaactgc aagggacttc cccataaggt
     2401 tacaaccgtt catgtcataa agc
//
LOCUS       DAPGRN010000247         2412 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPGRN010000247 DAPGRN010000000
VERSION     DAPGRN010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.252"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..321)
                     /locus_tag="RHW33_004743"
     CDS             complement(<1..321)
                     /locus_tag="RHW33_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV1648152.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRAK"
     gene            complement(554..1285)
                     /locus_tag="RHW33_004744"
     CDS             complement(554..1285)
                     /locus_tag="RHW33_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV1648153.1"
                     /translation="MNQPIHNAYWLSRFDSLLDSALSQHRAVSLIREDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATSLGYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFSEPSSLATMIQEAWCSALHLEPWQGDGLVHFSRWTPSRKPA
                     SSDARPSSDDTPLSGGCSDTRKASDKKSGEAAVLWIKRGDVEAMQKALERARYLVKNE
                     TKLHDGSGQRNYGCSRWSGHLLDGR"
BASE COUNT          741 a          572 c          506 g          593 t
ORIGIN      
        1 ttttgcccgt ccatattcaa cggcttcttt gaggagttca ttttccatcg ttttcttgcc
       61 gagcaggcgc tggagttctt taatctgctt catggcggca gcaagttcag aggcaggaac
      121 aacctgttct ccggcggcca cagcagtaag acttccttcc tggtattgct tacgccagag
      181 aaataactgg ctggctgcta caccatgttg ccgggcaacg agggagaccg tcatccccgg
      241 ttcaaagctc tgctgaacaa ttgcgatctt ttcctgtgtg gtacgccgtc tacgtttctc
      301 cggccctaag acatcaatca tctgttctcc aatgactagt ctaaaaacta gtattaagac
      361 tatcacttaa ataagtgata ctggttgtct cgagattcag ggggccagtc taggtgtgaa
      421 aagcaagcgt gcgaacggaa gtagatggca attcattgca ttacttcaga aaaacttttt
      481 caggaaacga gctaagggcg tcaaccaacg ccctgtagca taagctcagg caccggatgc
      541 cgttttacag gcttcacctg ccatccagaa gatgtcccga ccaacgactg caaccataat
      601 tacgttgacc tgaaccgtca tgcagcttcg tttcattttt cacgagataa cgggctctct
      661 ccagggcttt ctgcatcgct tctacatcac cacgcttgat ccagagaaca gcggcttcac
      721 cagacttttt gtctgaagcc ttcctggtat cagaacatcc acccgacaaa ggcgtgtcat
      781 cggaagaagg gcgagcatca gacgatgctg gtttacggga aggcgtccac ctggaaaaat
      841 ggactaggcc atcgccttgc cagggctcaa gatgcagggc gctgcaccat gcctcctgga
      901 tcatcgtcgc cagcgaagac ggttcgctga aatccccgag cgagcaccag gtatctttgt
      961 tgagcagtaa tattacgtga taatgtttcc tgccatacat cttgccgaac tcccggcacc
     1021 agaaataacc caagctggtt gcatgcacac gctgattggc acatcttttt ttccgctggt
     1081 aagcctgaat tttggctttc agggatgcaa aaaaacgaga aattaccgct gaatccggat
     1141 cgggatccat gatggtgaca ggcatatact cagggaaacg taaatcctcc cggattaacg
     1201 agacggcacg gtgctgcgac agagcactgt cgagaaggct gtcaaaacgg gataaccagt
     1261 aagcattgtg tattggttga ttcatgatga gagatctccg ttgatttgga ttgacttcac
     1321 atcattaatc accgcggtaa aactttacgg catccggcat tgagcaatgc tacaactctc
     1381 tgattaagag tgattgaaac ggaacaaacc atcccggaac gaaaagataa tcctacacgc
     1441 agggtaaaaa cactcaagaa cagcgaatgg atttaaaaac gacatggaca acgagaaaag
     1501 tcagaaggga aaaggatcca cagaacattc gaacaaaaca ccagaaacgc aaccagaacg
     1561 cttcctggta atgcaaatca acaacaaccc caaaacctac agcttaatca cacaactcaa
     1621 tcacggatgc gaatgacacg tttaaaacct gtttacagat aacgccgtta cacgatcatc
     1681 cccatcagaa tcgattttcc cttccggaaa gcgttatgtt tcactacgca ccataaaaac
     1741 gattaaccat cgataacgta aacttcatct gaatcaaaac accaaaaccg ttcatcacac
     1801 tttcgcaatg caagaagaaa cctcatgacg aaagcaataa ccttacacgg aaaagaaaaa
     1861 cgtcaggaat cgttaatgcc tcaatctgat tgttatcaaa acatccagaa agtcgtttaa
     1921 cagaatcgtc gtaagtatat cgaaaagaac gtcagattac ttcaggaaag ttcgggttga
     1981 tttagcgtaa agatactttt ctcatgccaa cgtcgaaact cgaaatctca ggaaaggtta
     2041 aacgtaacat cagtaaaatc gaatagcaga ttgattgctg aagcagaaag gttttacagc
     2101 agcattaaac tgatttagtt aacgtaaaga tttactaaac tggatgtgta tagcaatgct
     2161 taacgaaaac actcgtagaa gtgtttatga aaatacgtgc ataacactta ctgaaatgct
     2221 tagttaaata cgtgtatata gtatcatacc gacattagca acattctcag acactcatgc
     2281 agactgaatg ttgattaccc gttttcacat caggcatggt tctttactga ccaaaaaaca
     2341 aactggttta ctcttgaatt attttctcta cctcagaaaa catggtctca ggaatctgct
     2401 atggtttcat ag
//
LOCUS       DAPGRN010000248         2389 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPGRN010000248 DAPGRN010000000
VERSION     DAPGRN010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.253"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            371..1402
                     /locus_tag="RHW33_004745"
     CDS             371..1402
                     /locus_tag="RHW33_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV1648154.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRAITFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYAGRKYAFRPEKQRLLDAIWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKKLPADQQIHEMAEYLRKRLPPDEAYFCSDDHLKRLAIRELRQLE
                     LTLAAPPPH"
BASE COUNT          637 a          635 c          631 g          486 t
ORIGIN      
        1 aggaggcact ggaagagaag agatcgttgt aatgcttttc aaattaacgt aaagcgggta
       61 tatttcggct gttattagct gtgcagaggg tggcactctg tggagcaaag cggcgaaagc
      121 cggacggcag aatgcaccat aaggcattca ggagagatgg catgtacggg cagtaagtca
      181 gaagactgaa gatgttccgg aagccataaa aggaaaaccc ccactatctt tcttacgaac
      241 ttggcggaac gacgaaagat agtgggggcc tcacagaata cgggtaaagt ataatgaaac
      301 cgtaccagag attcaaccct gtgcagtgta taaatacacg gcacaatcgc tccgccataa
      361 gcgacagctt gtggcaggtc tgaagaatac tccatataac gcagtacact ggagtcagtt
      421 agcacccgaa gagcagatcc gtttctggga agactatgaa gcgggaaggg cgatcacttt
      481 cctggttgaa ccggaaagga agcgcacgaa gcgtcgtcgt ggtgagcact ccaccaaacc
      541 caaatgcgaa aatccgtcct ggtatcgtcc tgagcgctat aaggcgctga gcgggcagct
      601 cgggcacgcc tacaaccgtc tggtgaaaaa ggacccggtg accggcgagc agagcctgcg
      661 catgcacatg tctctgcatc ctttttacgt gcagaaacga acgtatgccg gtcgcaaata
      721 tgctttccgt ccggaaaaac aacgcctcct cgatgccatc tggccggttc tggtcagctt
      781 cagtgatgcg ggcacacata ccgtaggcat gagtgtttcc cgtctggcca gagaaatcag
      841 cccgaaagac agcaagggga aggttattcc ggaactggaa gtgacggtct cccgcctttc
      901 ccgtttgctg gccgaacagg tacgttttgg tgtactgggt gtttcagagg aaaccctgtg
      961 ggaccgtgaa acccgccagc gtctgccacg ttacgtctgg ataacaccgg caggctggca
     1021 gatgctgggc gtcgacatgg taaaacttca cgaacagcag cagaaacgac tgcgtgaaag
     1081 tgaaatccgc cagcagctca ttcgggaagg tgttctgcgt gaggatgaag atatctccgt
     1141 acatgcggcc agaaaacgct ggtatctgca gcgcagccag gatgcactga aacaccgtcg
     1201 tgcaaaagcg gcagccagta agcgcgccag acgcctgaag aaactgcctg ccgaccagca
     1261 gattcatgag atggcagagt atctcaggaa gcgtctgccg ccggatgaag cctatttttg
     1321 ttccgatgac catctgaagc gactggccat cagggagttg cgtcagcttg aactgacgct
     1381 ggctgccccg ccaccgcact agacagtacc attccctcag cactgaatca tcgccagccc
     1441 ctccggggct ttcggcgctg cttccgctta gcccaaaatc cgcagtaatc atcttaaatc
     1501 ccctcagagg ggcatatctg tccataaaac cacgcatcag tcatcagaac gtggccacgt
     1561 tgtttcagtt atccacataa atccgcaaac taagaacttt aagaagctgc aagcctaaaa
     1621 cagcaaacct gcaatatagt cttaacccca ttatttaatc ccctgcgttg cttcgccgca
     1681 gggaaagtct ttatctctga ggccactgtg aacaaataca aagaggcctt cgcttgcagc
     1741 ggccaaggcc gcgccgctca gaatttaaaa gtacctccca cgctaacgcg cgggccccga
     1801 acctcaccat tctgaaacca cagcaaaaag acatcaggaa taaaaaacac cacacaaacg
     1861 cagcaccgtg ctcatcctca taactgaaaa gcgaggccgc ccccgcccga agggcgggaa
     1921 caacatcgct tttaattatg aatgttgtaa ctacacgaca tcatcgctgc cagtcttctg
     1981 gctggaagtc ctcagtacac gctcgtaagc ggccctgacg gcccgctaac gcggagatac
     2041 gccccgactt cgggtaaacc ctcgtcggga ccactccgac cgcgcacaaa agctctgtca
     2101 tggctgaaag cgggtatggc ttagcagggc cggggatggg taaggtgaaa tctgtcaatc
     2161 cgtaccggct tacgccgggc ttcggcggtt ttactcctgt gccatatgct acaacggagt
     2221 gccgccttcc atgccgctgg cgcggcatat cctggtgacg atatttgaat cgttatatac
     2281 tgtgtatata cgtggtaatg acgaggtgat aaatggcaca ggttaatatg agtttaagaa
     2341 tcgacgctga actgaaggat gcttttatgg ccgctgcaaa aagcatgga
//
LOCUS       DAPGRN010000249         2293 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPGRN010000249 DAPGRN010000000
VERSION     DAPGRN010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.254"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..1472)
                     /gene="pykF"
                     /locus_tag="RHW33_004746"
     CDS             complement(60..1472)
                     /gene="pykF"
                     /locus_tag="RHW33_004746"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV1648155.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2029..2238
                     /gene="fumD"
                     /locus_tag="RHW33_004747"
     CDS             2029..2238
                     /gene="fumD"
                     /locus_tag="RHW33_004747"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HDV1648156.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          598 a          508 c          567 g          620 t
ORIGIN      
        1 aaataaaaaa agcgcccatc agggcgcttc gatatacaaa ttaattcaca aaagcaatat
       61 tacaggacgt gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc accagaaacc
      121 ataactacaa cgtcaccttt gtgtgccaga ccgctctgca gagccagttc tttacccaga
      181 cggtagaaat catcagtaga agtgatctct ttaaccagct gcggcacaac gcctttgctc
      241 agtaccaact gatgagccgt tttttcgtta gtggtcagtg ccaggatggt ggcatccggg
      301 aagtatttac gtactgcgcg agcagattta ccgccctggg tagcaaccac gatcagcgga
      361 gcatccagtt tttcagcagt ttcaacggca ccacggcata ccgcttcggt aatgcgcagt
      421 ttacggttgt cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt acgttcgcag
      481 atggtcgcca tgatagaaac cgcttccagc gggtatttac cttttgcgga ttcaccagac
      541 agcatcactg cgtcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc ttctgcgcga
      601 gtcgggcgtg ggtttttgat catggaatcc agcatctggg tcgcagtgat aacgacttta
      661 cgtgcacgga tacatttttc gatcatcatc ttctgggcga agataacttc ttctaccggg
      721 atttctacac ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc gaggatttcg
      781 tcgaagttgt tgaggccttc ctggttttcg attttggaga tgatgtggat gttttcgccg
      841 ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag aacgcttacg aataaaggaa
      901 gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt gtctttttca
      961 gccagtgctg gcagggcaat ggaaacgcca ggcaggttca cacctttgtt ttcacccagg
     1021 tcgccgttgt tcagcacttt acagataact ttgttacctt caatggcagt aacttccata
     1081 ccgatcagac catcgtcaac cagtacggtg ttgccaacag acaggtcagt agtgaaacct
     1141 tcatacgtta ccgcaaccat ttcgctgttg ccgataacag atttatcagt ggtgaaagta
     1201 aaggtctgac cagctttcag agaaacgtcg ttaccgcctt ccagtttcat ggtgcggatt
     1261 tccggacctt tggtatcaag caggatagcg gcggttttac cagttttgct catcacgttg
     1321 cgcagattct gaatgcgctg accgtgttct gcatagtcac catgagagaa gttcagacgc
     1381 ataacgttca tgccagcgtc cagcatttta gctaacatct cttcagattc ggttttcggt
     1441 ccgatggtgc aaacaatttt ggtctttttc atgacagtct tagtctttaa gttgagaagg
     1501 atgggagaaa cttgctttct gggcgcactg ccggaaagcg aatccggtat tacgaaagtg
     1561 gtggtgcctc gctctaagga taggtgacac cgaaaaagcg tgcagaggaa tgtgtgcttg
     1621 tgtctcagcc caacgtgagt gcctgttttt gagtcgttga gttctacagt ccaaagtgct
     1681 gaaaccattc aagagtcaat tggcgcgcat tatacgctga aattaatcca aaaggaaaac
     1741 aaaaacagcg tttcaaaaaa tatacaactt gttagacaat aaattgttat caaagattta
     1801 acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg cgcaacgtct ggtgccgggg
     1861 ttgtcactgt gaggataagc gaaagttcat ccggtgtata aatacatcat cgctcaattg
     1921 ctacagttaa aataatttat cgcgttattt gtctgatttt gtttgatttt tgtttaattg
     1981 gttagattga tatatgctga tagttggcat gataaatagg tataaagaat gggaaatcgg
     2041 acaaaagagg atgaactgta ccgagaaatg tgcagagttg tcggtaaagt tgtgctggaa
     2101 atgcgcgatc tggggcagga accaaagcat attgttatcg caggcgtatt acgtacagca
     2161 ttagccaata aacgcattca gcgcagtgag ctggaaaagc aggcgatgga aaccgtcatt
     2221 aacgcactgg taaaatgacc attcaggatg aggcggtaag cttcatcctg atattcttgc
     2281 ttcagttgcg gtc
//
LOCUS       DAPGRN010000250         2288 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPGRN010000250 DAPGRN010000000
VERSION     DAPGRN010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.255"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..236
                     /locus_tag="RHW33_004748"
     CDS             <1..236
                     /locus_tag="RHW33_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV1648157.1"
                     /translation="TLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFW
                     QPGTRAVMFSQPASQLLAGARMDVYVIRDGEGN"
     gene            236..1663
                     /locus_tag="RHW33_004749"
     CDS             236..1663
                     /locus_tag="RHW33_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HDV1648158.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGALYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            1653..2240
                     /locus_tag="RHW33_004750"
     CDS             1653..2240
                     /locus_tag="RHW33_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HDV1648159.1"
                     /translation="MANNMSLRQICHATRYVVAQGLCWLFWCLKYAVILPLATMALLA
                     LLVLWKDNTTPGKLLVQEIEFVRGRTPAGLYPVRYCSNSFGSDLAMPPSVTCGFRRGD
                     AAEYARETDQSLMQLFTALWATLALMYVSLAVISGKYPMRPGKMKCVRVATADEHLKE
                     VLTEDASLPEKIRKCHVYFPDDRTNRSNGDKNEYA"
BASE COUNT          543 a          570 c          703 g          472 t
ORIGIN      
        1 aaaccctgca ggccccggcg gggctgtctt cagtggcaga tgccgtatgg acggggaatc
       61 acctgaagat ggtccgcttt gccgtggaaa ataaaacgct gtctgccctg aatatccggg
      121 aaagtgattt ctggcagccg ggaacccgtg ccgtgatgtt cagccagcct gccagccagt
      181 tactggcagg tgcgcgcatg gatgtgtatg tcatccgtga cggggagggc aactgatggc
      241 cagtatcaat accattgtga aacgcaagca gtacctgtgg ctggggattg tggttgtcgg
      301 tgcagcctcc gcgattggtg gtgcactgta tctgtctgat gtggacatgt ccggtaacgg
      361 tgaggccgtg gctgaacagg aacccgtgcc ggatatgacc ggtgtggtgg atacgacctt
      421 tgatgacaag gtgcgtcagc atgccaccac agagatgcag gtgacggcag cgcagatgca
      481 gaagcagtat gaggaaatcc gtcgtgagct ggatgtcctg aacaaacagc gcggtgatga
      541 ccagcgtcgt attgaaaagc tgggacagga caatgctgca ctggcagagc aggtgaaagc
      601 cctgggtgca aatcctgtca ccgcgaccgg tgagccagta ccgcagacgc cagcctcacc
      661 gcccggcccg gaaggcgaac cacagccagg aaacacaccc gtatctttcc cgccgcaggg
      721 cagcgttgct gttccaccgc cgacggcgtt ttatcccggg aatggtgtca cgccaccacc
      781 gcaggtgacg taccagtctg taccggtgcc taaccggata cagcgtaagg tgttcacccg
      841 taatgaggga aaacagggac catcactgcc gtacattccg tcaggaagtt ttgcgaaagc
      901 catgctgatt gaaggagcgg atgccaatgc gtcggtcacc ggtaatgaat ccacggtgcc
      961 gatgcagctg cgtatcaccg gcctggtgga aatgccgaac agcaaaacgt atgacgcaac
     1021 gggatgtttt gtggggctgg aagcctgggg ggatgtctcc agtgagcgtg ccattgtacg
     1081 cacccgcaat atcagctgcc tgaaggacgg caaaaccatt gatatgccga ttaaggggca
     1141 tgtcagcttc cggggtaaaa acggtatcaa gggcgaagtg gtgatgcgta acggcaaaat
     1201 cctcggctgg gcatggggcg cgggatttgt tgacggtatc ggtcagggaa tggagcgcgc
     1261 ctcccagccg gctgtcgggc tgggtgccac cgctgcttac ggggccggcg atgtccttaa
     1321 aatgggtatc ggtggcggcg catcgaaagc cgcacagacg ctcagtgact actacatcaa
     1381 acgtgccgaa cagtatcacc cggtgatacc gattggtgcg ggcaacgaag tgaccgtggt
     1441 gttccaggac ggcttccagc tgaaaaccgt ggaagagatg gcgctggaac gcacgcagag
     1501 cagagcggaa gaagacaatc cggaaagtcc ggttcctgtt ccgccatcgg ctgaaagtca
     1561 tcttaacggc tttaatactg atcagatgct gaagcagctg ggcaacctga atccgcagca
     1621 gtttatgtcc ggcagccagg gagggggcaa cgatggcaaa taatatgtca ctccgtcaga
     1681 tatgccatgc cacccggtat gttgttgccc agggcctgtg ttggttgttc tggtgtctga
     1741 aatacgccgt cattctgccg ctggccacaa tggctctgct ggcgttactg gttttatgga
     1801 aggacaacac cacaccgggg aaattgctgg ttcaggagat cgagtttgta aggggcagaa
     1861 cgccagccgg gctgtatccg gtccgatact gctcgaacag ttttggttcc gatttagcga
     1921 tgcctccgtc tgtaacgtgc ggttttcgcc ggggggatgc tgcagaatat gccagggaga
     1981 ctgaccagtc tctgatgcaa ctgttcaccg cattatgggc aacgctggca ctgatgtatg
     2041 tctcgctggc ggtgatctcc ggaaaatatc cgatgcgacc cggaaaaatg aaatgtgtcc
     2101 gggtcgcaac tgcagatgaa catctgaagg aagttcttac ggaagatgct tccctgccgg
     2161 aaaaaatcag gaaatgccat gtttatttcc ctgatgaccg aacaaacaga agcaacggag
     2221 ataaaaatga atatgcgtaa tattaatatt attactgcac gctctgtacc ggataaaagc
     2281 gtatcaga
//
LOCUS       DAPGRN010000251         2210 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPGRN010000251 DAPGRN010000000
VERSION     DAPGRN010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.256"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..753)
                     /locus_tag="RHW33_004751"
     CDS             complement(<1..753)
                     /locus_tag="RHW33_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV1648160.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNTFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDED"
     gene            complement(779..1471)
                     /gene="rfaY"
                     /locus_tag="RHW33_004752"
     CDS             complement(779..1471)
                     /gene="rfaY"
                     /locus_tag="RHW33_004752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HDV1648161.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1481..>2210)
                     /locus_tag="RHW33_004753"
     CDS             complement(1481..>2210)
                     /locus_tag="RHW33_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HDV1648162.1"
                     /translation="YFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGS
                     LKPLTEIIFKDEFAAVIPDNDSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANL
                     TPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELYDRSHRKY
                     QQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQ
                     YKHLFAHGEYLKGITSLIKYKLKK"
BASE COUNT          691 a          439 c          381 g          699 t
ORIGIN      
        1 atcttcatcg cttttatttt ttaactcata atcaatgcca taaaagcaat cataatcgcc
       61 gctaataaaa atacaatgac caaagaacag aatatttaaa atatcctgat caaggcattt
      121 atacttcgtg tgctgcgggt cacttgccag catagcaatg gctttattca taaaatcatt
      181 tttcgcccat gcttcaagct gcagatatac tacaccggcg ttgaaataat tccctggaag
      241 tccctctata gccagacgct tcgctggttt ttcctgcata aaaggcacat ctttaataac
      301 agctgcgaac ttatccccat caaaattaat atcaagcaat ccagctaatg agcctttaca
      361 gatgacatca gcatcgatat ataacagatt ttcaatagtt tcactcaaat actggaacgc
      421 aactaaacgg aagaatgttg cataagacca ctgtttcagg ctggggaaga tatcaataaa
      481 ggcattggag actttataaa ttattacttc aacgttagca tttttgctgg taaaagtatt
      541 aacacgttgt agataatctt catcaatgta gtcggtaaag agatgaaact taaccgcaag
      601 gtccgggtta tgcataacaa ctgattgcat agatactgcc gcaccaaata aaaagttacg
      661 gtcaataccg taagcgatat ttaacacacg atcggtgtta gcaatttccc cagagacagc
      721 gacttcagta atactctcag ctaataaatc catgttggtt ccgttttgac tgtgtggttt
      781 acgctttgcc ttttaatttt ttaataaatt tgcgtagttt ggttctgtag attactgaat
      841 agtagccata atcttttatt tcgttggcaa tacccaggtg acgttccatg gccagacgat
      901 cacgagcttt acgctctgcc gtacagcttt taccggatag gtcaattatc ctgacaccat
      961 ctttactaac gataaagtta ccccggtgtg ggtcgcccga tagcatattt aaggcatgca
     1021 atttttccat tgaagcctta atttctgctt taacattttc tggaataatc ggcatatcat
     1081 tgagctctac gccctcgaca tactcgataa gcatgataaa aacactggca tagttgaaaa
     1141 ttttacgctc agccagaaaa taaaaatcat taggaaaagt aagacctgca cttctgacac
     1201 gatcggtttc aacgatcaaa ttctgatagt agtcaccttt tacaaaagat tttaaaaaac
     1261 gttcattcct tttcgttttc ggagcaaaga ctttaaaaac atagcgacca tatttcgtat
     1321 caatcaacga tactttggta tcatcaatat tacgtaatac ttttactgtt ttaaaattat
     1381 aggccagtat ttcatcaaag atttctttat atttattatc attatctttg taataaaatg
     1441 agaagccgcg ataacgtata cttgtaatca taagagtaat ttatttctta agcttgtact
     1501 taattaatga agttatgcct tttagatact caccatgggc aaacagatgc ttatattgtt
     1561 tctgcatttc tgcaacagtc cgcgcctctt taagaggata tttcttccag ggagattgtt
     1621 cacgcgcgat attaaagtat gatgcagacg gatatccagc ccagctatgc catggtttag
     1681 ttatccctgt atagtgaatc aacactgttt tatcggtaat ggtttgctga tactttcgat
     1741 gactacgatc ataaagttcg tttttcaggg tataaatagt atcaaaatcc ttcgcaaggt
     1801 agatattatt catattaaat gcgatattca acgcatcctg atctaaatat ttaagagagc
     1861 catatttagt ttcccctcgt aaaagtttga gtaaatacgg tgtcaaattt gcttcatgcc
     1921 attttttaag attgacatag ataacgcctg cattgaaata acgtccattc atttcgggaa
     1981 tgttgaggcg ttttgcacat gccgcctgag tactatcatt gtcaggaatg accgcagcaa
     2041 actcatcttt aaatataatt tctgtgagag gtttcaggct tcctttacaa acaacatcgg
     2101 catccagata cagcagtgtg gaaatacttt cactcagata ttcaaaagac aataccctga
     2161 agtatgtcgc gtacgaccaa aactgtgacg tcggcagatc agcaaagtat
//
LOCUS       DAPGRN010000252         2198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPGRN010000252 DAPGRN010000000
VERSION     DAPGRN010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.257"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(98..1621)
                     /locus_tag="RHW33_004754"
     CDS             complement(98..1621)
                     /locus_tag="RHW33_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1648163.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGTVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQNLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(1957..>2198)
                     /locus_tag="RHW33_004755"
     CDS             complement(1957..>2198)
                     /locus_tag="RHW33_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648164.1"
                     /translation="SIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLKDET
                     SVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          660 a          488 c          463 g          587 t
ORIGIN      
        1 aattatagca aacaaaataa tgaacaatag taacaagagg caacgagcta attagctgtc
       61 tataattaca ccgcactatg gccttactga tcgattttca ctcaaccacg accttcacct
      121 tcggtttaga aaggataatt ttgaccaagt cgatgtatgt aacaggttta tagaaataat
      181 aaccctgctg aaatgtgata ttattttggt tcagataatc aagttgttct ttcgtttcga
      241 caccttcagc gacgatactg atcgaaagtt tacgcgccag atccaataca caatccagaa
      301 ttcgggttga ttctgggtct gcgttgacgc ggccaacgaa actgtgatca attttgatat
      361 aatcaatatg caagtcatgc agataagaaa gccctgagta gccagtaccg aaatcatcca
      421 gcgcgatgac aaaaccattt tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa
      481 catttaatgg ctcacgctcg gtaacttcca gaacgaggtt taaatcgcgg cgggtaaaac
      541 tgtcacggta atttaagcac tcgtcgacaa acgtcggcga aataatatgc gaggcgctaa
      601 aattaattcc aatatgaaaa ccttccggca atttactcgc gatagcgttc atctgtctgg
      661 caacctgatt catcaggttt tgcgtaagcg gaacgattaa tccggatttt tcagcaagtg
      721 gaataaatgc cgcgggtgaa atatatccac cgtgaggttg tttccagcgg gctaacacct
      781 caactccccg caatgtccct tcccgaccat tcaccactgg ttggtaaaaa gggacgatct
      841 ccccattatt tatagcccgc cgcaggatct cttcaggcgt tgcgctttta ttgaaatacc
      901 tatctagcag gaaggcagcg gcgcaggcga ttaacagaat aaagatcaaa atcccaaagc
      961 ctttatcgat aagtctgtag aagctaaaga gtggcggcga gttaaagata atagtgaaat
     1021 gatattttgt ggagtgaacc ttctcggtat agggataatg tccattaagc gtcatcacat
     1081 cacccgtagg tccaataatg tcatccgcca cgcgtaatac ataccttacc cctttcaagg
     1141 gtacattaag cgccccgcga atatgctgat cacttatcgt aaccaaaatg cgcgtatctg
     1201 caaattggct ctgatagagc aggataggta atctattaac ggtgtcgatt gccggagcca
     1261 acagtaaatt actgtccggg aaaacaggaa tacgagacag caggacccga ttccccggta
     1321 aggatgtaca ccagaccgtt ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca
     1381 gagctgcttc agtgccaagc tgatattgct cctcgaggtc gcattccttc ccggcaatgt
     1441 taaccgctgt ttgagtcgcc tgtgacgctt cttcaagtat atgattgata ttgttggcgg
     1501 catatcttgc tcccgccagg tactctgctt ttgcggaata ccagagctgg atgttcaaaa
     1561 tagcgacacc ggttataaac aagcatatcg caacgaggat gggtatgagt gtattgcgca
     1621 ttcgcttgtc ccgaacaaag ggtttaatac atgaaaatga cgttcattcg ctcgcgctgg
     1681 cgtctacaga gagcatggta gagagcatca caaatatcca cgccgaagat aaaattttct
     1741 ccatcttcat ctaacctatt aattattgag cacaatgtag gcagaattaa acaaattagg
     1801 tgggatatca ggcgtcaaga atggaaaacg agcgctctcc attcctgttt gccccctgat
     1861 attgcggata tattaagaaa gcataacgcc tgaaatgctc actttgcacc agcatgatga
     1921 tacagctgat gtttattcta taacgttact caagttctaa gagagcacgg attccctgtc
     1981 attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca agatttgcag
     2041 atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta aagcctgatc
     2101 aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga gatttttaaa
     2161 caaagcacat tgaaaaataa atctctctgg gattgaat
//
LOCUS       DAPGRN010000253         2161 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPGRN010000253 DAPGRN010000000
VERSION     DAPGRN010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.258"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            241..489
                     /gene="repA"
                     /locus_tag="RHW33_004756"
     CDS             241..489
                     /gene="repA"
                     /locus_tag="RHW33_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HDV1648165.1"
                     /translation="MSQTENAVTSSSGTKRAYRKDNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCEAEGVTQAEMIAELIRQKSAFS"
     gene            736..810
                     /gene="tap"
                     /locus_tag="RHW33_004757"
     CDS             736..810
                     /gene="tap"
                     /locus_tag="RHW33_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HDV1648166.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            803..1660
                     /gene="repA"
                     /locus_tag="RHW33_004758"
     CDS             803..1660
                     /gene="repA"
                     /locus_tag="RHW33_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HDV1648167.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEVAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANREAIKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          569 a          572 c          535 g          485 t
ORIGIN      
        1 ggtatttcag gggcagacac ccgacatgcc agaaacagcc ggtcccgccc ggggccggca
       61 cacaggttaa ggcatttcca tcttttcagt catttcatta tcaaaatcac attaaacggt
      121 cgtaatcaga catgatttgt gcgccaacac agatcatcgt cacaactttc aagtcgctga
      181 tttcaaaaaa ctgtagtatc ctctgcgaaa cgatccctgt ttgagaattg aggaggcgag
      241 atgtcgcaga cagaaaatgc agtgacttcc tcatcaggta caaagcgagc atatagaaaa
      301 gataatcctc tgacgcttgc agaacgtcag caggcatccc tggcaagaaa acgagccaca
      361 cataaagaac tcagagtgtt tattcctgct gcgttaaaag cgcaattgca ggagatgtgt
      421 gaagctgagg gtgttactca ggctgagatg attgccgaac tgataaggca gaagagtgct
      481 tttagctaac tgaaatgtag cgacatcaca ttcttgcctg tttctggtgt gcgtgataga
      541 ttgctgatcg tttaaggaat tttatggctg gccacgccat aaggtggcaa ggaactggtt
      601 ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg ataatcttct ttaactttgg
      661 cgagtacaga aagattaccg gggccatcta aaaaccgtat agccagcaat tcagctatgc
      721 ggggagtata gctatatgcc cggaaaagtt caagacttct ttctgtgctc actccttctg
      781 cgcattgtaa gtgcaggatg gtgtgactga tcttcaacaa acgtattacc gccaggtaaa
      841 gaacccgaat ccggtgttta caccccgtaa aggtgccgga acgctgaagt tctgcgaaaa
      901 actgatggaa aaggcggtgg gcttcacctc ccgttttgat ttcgccattc atgtggcgca
      961 tgcccgttcg cgtggtctgc gtcggcgcat gccaccggta ctgcgtcgcc gggccattga
     1021 tgcgctgctg caggggctct gttttcacta tgatccgctg gccaaccgcg tccagtgctc
     1081 catcaccacg ctggccattg agtgcggact ggcgacagag tccggtgcag gaaaactctc
     1141 catcactcgt gccacccgtg ccctgacgtt cctgtcagag ctggggttga ttacctacca
     1201 gacggaatat gacccgctta tcgggtgcta cattccgacc gacatcacgt tcacaccggc
     1261 actgtttgcc gctctcgatg tgtctgaggt ggcggtggct tccgcacgcc gcagtcgtgt
     1321 ggaatgggaa aacagacagc gcaagaagca gggactggat gcactgggta tggatgaact
     1381 gatagcgaaa gcctggcgtt ttgtgcgtga gcgtttccgc agttaccaga cagagcttaa
     1441 gtcccgtgga ataaaacgtg cccgtgcgcg tcgtgatgcg aacagggaac gtcaggatat
     1501 cgtcaccctg gtgaaacggc agctgacgcg tgaaatctcg gaagggcgct tcactgccaa
     1561 tcgtgaggcg ataaaacgcg aagtggaacg tcgtgtgaag gagcgcatga ttctgtcacg
     1621 taaccgcaat tacagccggc tggccacagc ttccccctga aagtgacctc ctctgaataa
     1681 tccggcccgc accggaggca tctgcacgcc tgaagcctgt cggcgaacaa aaaaacagca
     1741 ccgcatacaa aaaacaacct catcatccac cttcaggtgc atccggtccc tcctgttttt
     1801 gatacaaaac acgcctcaca gacgaggaat tttgcttatc cacatttagc tgcaagggac
     1861 ttccccataa ggttacaacc gttcatgtca taaagcgcca tccgccagcc ttacaggatg
     1921 caatgtatct tttaaacacc tgtttatatc tcctttaaac tacttaacta cattcattta
     1981 aaaagaaaac ctgttcactg tctgtcctgt ggacagacag atatgcacct cccaccgcaa
     2041 gcggcgggcc ccgaccggag ccactttagt tacaacactc agatacaacc accaaaaaaa
     2101 acccggtccc gcgcagaact gaaaccgcaa agccccccct cataactgaa aagcggcccc
     2161 g
//
LOCUS       DAPGRN010000254         2157 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPGRN010000254 DAPGRN010000000
VERSION     DAPGRN010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.259"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            144..2129
                     /gene="yjcS"
                     /locus_tag="RHW33_004759"
     CDS             144..2129
                     /gene="yjcS"
                     /locus_tag="RHW33_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV1648168.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKVYYRADDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVKALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSIRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          577 a          561 c          538 g          481 t
ORIGIN      
        1 acaaatgcct aataaaatgg atcgttctgc tcacacttcc acttattaaa gtcgtaattt
       61 cccaattatg ataacgccag ctttaaatga cgtattttgc atggattaca tatttacgac
      121 ataaatgata aatggagatt tatatgaata actctcggtt attccgtttg agcaggattg
      181 ttattgcgtt aactgccgcc agcggcatga tggtaaatac cgctaacgcg aaagaggaag
      241 cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa tcattaccgt
      301 ttagcgatcg tcaggatttt gacgatgccc agcgtggatt tatcgccccg ctgctggatg
      361 aaggtattct gcgtgatgcg aacggtaaag tttactaccg cgcggacgat tacaaatttg
      421 atattaatgc cgcagcgccg gaaaccgtaa accccagcct gtggcgtcag tcgcagatca
      481 acggcatttc tggcctgttc aaagtcaccg ataaaatgta tcaggtgcgc ggccaggata
      541 tctctaacat tacgttcgtt gagggcgaga aaggcattat tgttatcgac ccgctggtga
      601 cgccacctgc cgcaaaagcc gcacttgacc tttacttcca gcatcgtccg caaaaaccga
      661 ttgttgccgt tatctacact cacagccaca ccgaccacta tggtggcgtg aaaggcatta
      721 tctctgaagc cgatgttaaa tccggcaaag ttcaggtgat tgcccctgct ggctttatgg
      781 acgaagccat cagcgaaaac gtgctggcgg gtaacatcat gagccgccgt gcgctctact
      841 cttacggtct gttactgcca cacaacgcgc aaggcaacgt gggtaatggc cttggcgtga
      901 cgctggcaac gggcgacccg agcattattg caccgactaa aactatcgtc agaactggcg
      961 agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt agcgaagcac
     1021 cagccgaaat gcacttctat attccggccc tgaaagccct gtgtaccgcc gaaaacgcca
     1081 cgcataccct gcacaacttc tacactctgc gcggcgcgaa aacccgtgac accagcaagt
     1141 ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa gtgctgttta
     1201 tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt ggtaaatatc
     1261 gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag ggctacacca
     1321 tgaatgaaat cggcgacatg attaagctgc cgcctgcact tgccaataac tgggccagcc
     1381 gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc tatcttggct
     1441 attacgacgg taacccggct aacctgcatc cgtatggtca ggtagagatg ggcaaacgtt
     1501 acgtgaaagc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc
     1561 aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc aacccgggtg
     1621 accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat caggccgagt
     1681 ccgccacatg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata
     1741 agttcagcca cggcaccacc ggttccccgg acaccattcg cggaatgtcg gtcgaaatgc
     1801 tgttcgactt tatgtccatt cgcctcgata gcgaaaaagc cgcgggtaaa aatatcagcc
     1861 tgaacttcaa tatgagcaac ggcgataacc tcaacctgac gctgaacgat agcgtgctta
     1921 actaccgtaa aacactgcaa ccgcaagccg acgcctcttt ctacatcagc cgtgaagatc
     1981 tgcacgccgt gctgaccgga caggcaaaaa tggcggatct ggtgaaagcg aagaaagcca
     2041 aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat aatttcgatt
     2101 tgtgggttaa tatcgtaacc ccaaattaat gtcactcgct tttgggggcg ctgaagc
//
LOCUS       DAPGRN010000255         2127 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPGRN010000255 DAPGRN010000000
VERSION     DAPGRN010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.260"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..1331
                     /gene="sctN"
                     /locus_tag="RHW33_004760"
     CDS             39..1331
                     /gene="sctN"
                     /locus_tag="RHW33_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
                     /protein_id="HDV1648169.1"
                     /translation="MKKLKLLNKYSYLHSINGSLIEAELDDVSVGEVCEIYASWQANE
                     RIARAQVVGFRNGKTLLNLIGSSVGLTRTAILKPTGEQLTIQISDAFLGSVLNASGQI
                     MERFVPDTPGDRGNLRLIDELPPSYQERRVINTPLETRIRVIDGVLTCGIGQRVGIFA
                     SAGCGKTVLMHMLVNNTEADVFVIGLIGERGREVTECAESLKQSVNAAKCVLVYATSD
                     FSSVDRCNAALMATTVAEYFRDRGKRVVLFIDSMTRYARALRDMKLAAGEPPARRGYP
                     ASVFDSLPRLLERPGPTLKGSITAFYTVLLEGEDESDPLGDEIRSILDGHIYLSRKLA
                     GQGHYPAIDVLKSVSRVFGQVTDEKHRDNAARVRKILTTLEDLQVFIDLGEYRAGQNA
                     ENDFAMNARPKLTNWLKQSVNEKMPMSETLKELERIVK"
     gene            1321..1779
                     /locus_tag="RHW33_004761"
     CDS             1321..1779
                     /locus_tag="RHW33_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV1648170.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLQSLLDMKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYERLKTRIKKQLLDQMLYQDELEQEEKYNGRSQEN"
     gene            1757..1987
                     /locus_tag="RHW33_004762"
                     /pseudo
     CDS             1757..1987
                     /locus_tag="RHW33_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311755.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
BASE COUNT          711 a          380 c          495 g          541 t
ORIGIN      
        1 tatttaggat aatgtcttgc gatatggagc atgccggtat gaaaaaatta aaattattaa
       61 ataaatacag ctacttacat agcataaacg gctctctgat tgaagcagaa cttgacgacg
      121 tttctgtggg tgaagtatgc gaaatttatg cgagctggca agcaaatgaa cgtatagcca
      181 gggctcaggt cgttggcttt cgcaatggaa agacattatt aaaccttata ggcagctcag
      241 tggggcttac acgtactgca attttgaaac caacgggaga gcagttgacc atacagatta
      301 gtgatgcctt tcttggttcg gttttgaatg cctctggtca aattatggaa aggtttgtcc
      361 cggacactcc aggcgataga ggaaatttac gcctgattga tgaactgcca ccgtcgtatc
      421 aggaacgtcg agttattaat acaccacttg agacacggat aagagtcatt gatggcgtac
      481 tgacatgtgg cattggtcaa cgtgttggga ttttcgcctc ggctggctgt ggcaaaacgg
      541 ttttaatgca tatgctcgta aacaatactg aagctgatgt gtttgttatt ggcttgattg
      601 gcgaacgtgg tagggaagtt acggaatgcg cggaatcgct gaaacaatca gtaaatgcag
      661 cgaaatgcgt attggtatat gccacctctg atttttcgtc agtagaccgg tgtaatgccg
      721 ccctgatggc gacaaccgtg gcagagtatt ttagagatcg aggaaagaga gttgtacttt
      781 ttatcgactc aatgacgcgg tatgcacgag cactacgtga tatgaaactg gcagcgggtg
      841 aaccacctgc cagacgtggt tatcctgctt ctgtttttga cagtttgcct cgcttactgg
      901 agaggccggg accaacacta aaaggaagta tcaccgcgtt ttataccgtg ttactggaag
      961 gggaagatga atcagacccc ctgggtgatg aaattcgctc aatccttgat ggccatatct
     1021 atctaagtcg caaactcgcg ggtcaaggtc actatccggc aatagatgtt ctaaaaagtg
     1081 tgagtcgagt atttggtcag gtcactgatg aaaaacacag agataatgct gcacgggtaa
     1141 ggaagatcct cacaactttg gaagatctac aggtgtttat tgacttaggg gaatatcgcg
     1201 ctgggcaaaa tgctgaaaat gattttgcta tgaatgcacg gcctaaactc acaaattggt
     1261 tgaaacagtc agttaatgaa aaaatgccaa tgagtgaaac tctaaaagag cttgaacgaa
     1321 ttgttaagta aagttaatcg acttataaga agaacagcgc agagcctggc tgcatgtgaa
     1381 gcatcattac aaaagttaaa tgcagaaaaa gaaaagttag ccgaaaagga acgcctgtat
     1441 gatatgcaac ttaaaaacct gcagtcactg ttagatatga aagagttact cggcgaagtt
     1501 gtatttcgac aggatatatt ttactcgtta cgtaaagttg cagtcattca gcagcagatc
     1561 gctgagataa atcttgaaaa gcaaaaaata gcagagcgga gaaaaatact taacaaggaa
     1621 atagttcaac agcaagcaca aagaaaacac tggtggctaa aaggagaaaa atacgaacga
     1681 ctaaaaacgc gcatcaaaaa acaactttta gatcagatgc tttatcaaga cgagcttgaa
     1741 caggaggaaa aatataatgg acgaagtcaa gaaaattgaa cacggtaaaa ttttaaacac
     1801 agacacaccc gataattctg cttcggattt agacagtttc ttaaagaaaa acaaaaaaaa
     1861 taaagaaatg agtgagattg tcgctggaac cattctccag ttattacgtc agactcagga
     1921 tagccaattt tctacgttgg ggaaaaaaaa tgggcaaatg aagtctattg acaacaaatc
     1981 atctctccag gttgacagga atccatatct ttctttgcaa acaaatggaa cagagcaatt
     2041 ttctacaaag aaaattctca atatgacttc tcgaggtcag ggtattagtt ctgaactatc
     2101 tgataacacg ataaaaatta aggaaaa
//
LOCUS       DAPGRN010000256         2055 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.261, whole genome shotgun sequence.
ACCESSION   DAPGRN010000256 DAPGRN010000000
VERSION     DAPGRN010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.261"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..311
                     /locus_tag="RHW33_004763"
     CDS             15..311
                     /locus_tag="RHW33_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648171.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            422..1243
                     /locus_tag="RHW33_004764"
     CDS             422..1243
                     /locus_tag="RHW33_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1648172.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRAPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1540..2049)
                     /locus_tag="RHW33_004765"
     CDS             complement(1540..2049)
                     /locus_tag="RHW33_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDV1648173.1"
                     /translation="MKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWKE
                     SRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAI
                     AFKKWGVTWEAVGAYNAGFRKTERQNQRRLAYASDVYRIYTGIKSSKGIRLPATKKSL
                     PEINSVQNN"
BASE COUNT          538 a          429 c          487 g          601 t
ORIGIN      
        1 atacaggagg aattatgagc gcattttctt ttaacaccac ttatcagcca acaggcgatc
       61 agcagaaaga cgcaatcgca caaatcggga tcatgcagaa tcgtgctgtt caggcaaatc
      121 ttgcatatca gagctgccgg gattctggtg cactctttaa ggtgctccac caggtcaata
      181 atgagctgca tgacttactg gatagcctgg agaatcatac tcctcttgtc agaaaaaacg
      241 ctgatgagtt gatcgcgctg ttactgcttt tcactcggca ggttggtcag tcccgaactg
      301 acctgatttg atggtccttc ccctcggaat tgttgtcttt ttgttaacga taaccgaggg
      361 gggatgatat taagcgaaaa aacttttatt aattttatgc cttcataaag gagttactgt
      421 tatgcgatta gcttcccgtt ttggtcgata taattctatt cgccgtgagc gcccgttaac
      481 ggatgacgaa ttaatgcagt ttgttccatc tgtcttttct ggtgataaac atgaatcccg
      541 gagtgagcgt tatacatata tcccaaccat caatatcatc aataagttac gtgatgaagg
      601 tttccagcca ttctttgcct gtcagagtcg ggttcgtgat ttgggacgcc gcgaatacag
      661 taaacatatg ttacgtcttc gcagggaagg gcatattaac gggaaagaag ttcctgaaat
      721 tatcctgctt aattcacatg atggttcatc cagttatcag atgatccccg gaatttttcg
      781 ttttgtctgc acaaatggcc tggtgtgcgg aaataatttt ggcgaaatcc gcgctccaca
      841 taaaggtgat attgtcgggc aggttatcga gggagcgtat gaagtgctcg gtgtctttga
      901 taaggtcact gataatatgg aggcgatgaa agaaattcat cttaacagtg acgagcaaca
      961 tttatttggc agagctgcac tgatggtcag gtatgaagat gaaaataaaa cgccagtgac
     1021 acctgaacag ataattactc cccgtcgtcg ggaagacaaa cagaacgatc tctggacaac
     1081 ctggcagcgg gttcaggaga atatgataaa aggtggatta tcggggcgaa gtgcctccgg
     1141 gaaaaatact aggacaagag ccattacagg tattgatggt gatataagaa tcaacaaggc
     1201 gttatgggtg attgccgaac agttcagaaa gtggaagtca tgataatata agtaaagccc
     1261 cggaaaaatt ttcggggctt tacttatatt atcatttttc ggcattgctg tgtcgtctga
     1321 tatacctcaa tttcatcaac cggctttccg gttgtctgta aatattgtgc ccgcgaaaga
     1381 tggtctggca ggaggctgcg cagttaccgg gtatatataa gatgggttcc cgcttcgcgg
     1441 ggcttcggcc agtcagtatt gtcactcata tgatattttt gtgtgtggcc ttccatgccg
     1501 cttttgcggc ataaccggta tctgacaatg tctgtaagat taattgttct gcacgctgtt
     1561 aatttccgga agtgatttct tcgtggccgg aagccggata cctttactgc tttttatccc
     1621 agtataaatc cggtaaacat ctgatgcgta ggcaagacgt ctctggttct ggcgttcggt
     1681 tttcctgaat ccggcattgt atgcaccaac ggcctcccag gtgacgcccc attttttaaa
     1741 tgctattgcc agataataag caccggtata aatgttcatg cagggatctg ttgtcagatg
     1801 ttctggctta attccgtagc gggccagttc gttaaaatgc tgggaatcta cctgcatcag
     1861 tccgctgcca tatcccgtta ccggattaat accgatggca ttaactcggt aacgggattc
     1921 tttccatgat attgctctca gtaaatcagg atctattttg taatcccggc ctgcaagatc
     1981 aaagcaatca gtggcctggc agatctcatt tataaacatc aggcagatgg ctaacatcca
     2041 ttttttcatt tttcc
//
LOCUS       DAPGRN010000257         2014 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPGRN010000257 DAPGRN010000000
VERSION     DAPGRN010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.262"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            9..1367
                     /locus_tag="RHW33_004766"
     CDS             9..1367
                     /locus_tag="RHW33_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV1648174.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQMIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKASENTKQRNLFSKKMTLCISLFFVLG
                     GPIVAHILSSHYESYDLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNVSLLFMSRYSVSIWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKDIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMLMDIDHFKKVNDTWGHPIG
                     DQVIKTVVNIIGKSIRPDDLFARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQGNALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            1465..>2014
                     /locus_tag="RHW33_004767"
     CDS             1465..>2014
                     /locus_tag="RHW33_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HDV1648175.1"
                     /translation="MLIIDQSAITRFYWQLYYNFLGCITLMRGETCMVFNKKMFVLII
                     IPGILGVLLSFAMSVFQMNRDTTITAGILLKQLDNVTQIVKHTTKLTSILVMKPCKDI
                     LEQLIANGALTPYVRTTGLIENNFQICSSVSGFKKMNVNDVYGTSFHNKNKESRIVSI
                     SGTSFVPGKTAIVFLMPIGNDMT"
BASE COUNT          585 a          336 c          437 g          656 t
ORIGIN      
        1 gtagctttat ggaaaaagac tatttgagaa ttagtagtac tgtcttagtg agcttattgt
       61 ttgggcttgc tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg
      121 ttccacgatc aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat
      181 ttatttttat gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta
      241 gtttggcttt tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa
      301 tgattgaggg gcgtttgaca agcagtgttg ttcaaaatga cattgcaatt tattatttgt
      361 ttcgtcagat gagtttgtgc atattaatat ttctggcatt ggtgaataaa gctagtgaaa
      421 acacaaaaca gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg
      481 ttcttggggg gccgatagtt gctcacatac tttctagcca ttatgagagc tacgatttac
      541 acattgccga gttaaccaat gaaaatggtc aagtggtttg gaaagcttca tatgttacca
      601 taatgatttt catgtggtta accttgcttt cggttaatct atactttaac ggtttacgct
      661 atgatatctg gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatgtctctc
      721 ttttatttat gagcaggtat agcgtgtcca tttggtatat tagtcgcact attgaagtag
      781 tcagtaagtt aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa
      841 caaaggacat tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta
      901 atgaactgac ggttcaatca gcatcggcca aaaaaacgcc ttattgcgtc atgcttatgg
      961 atatcgacca cttcaaaaaa gtcaacgaca cctgggggca tccgattggc gatcaggtga
     1021 taaaaacagt ggtgaatatc attgggaaaa gcatacggcc agatgacctt tttgcgcgcg
     1081 tcggcggcga agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt
     1141 tagcggaaag gattcgggaa aatgttgagc gtttaactgg tgataatcct gaatacgcca
     1201 taccacaaaa agtgacgatt agtattggcg ctgttgtgac tcagggaaat gcgttaaacc
     1261 cgaatgagat ctatcgactg gctgataacg cactatacga ggcgaaagag accgggcgta
     1321 ataaggtggt tgtgagggat gtggtgaatt tttgtgagtc accataaagc ggcattttag
     1381 cgggtgattt tggtaagcgc taaatagcta ttcgcagaca tgcgtaacaa ttttcagaaa
     1441 tatcatagta ctatatgatc ttaaatgctt atcattgatc agtctgctat cacccgcttt
     1501 tactggcaat tgtactataa tttcttaggt tgcatcaccc tgatgcgtgg ggaaacatgt
     1561 atggtattca acaaaaaaat gtttgtactc attataatac caggcattct gggggtgtta
     1621 ctgtcgtttg ctatgtcagt atttcaaatg aatcgagata cgacaattac cgccggtatt
     1681 ctgttaaaac agcttgataa tgttacgcaa attgtcaaac atacgacaaa gcttacttct
     1741 attctggtga tgaaaccctg caaagatatc ctggaacaat tgattgctaa cggagcattg
     1801 accccctatg ttcgaacaac gggtcttata gaaaataatt tccaaatatg ttcttcagtt
     1861 agcggtttca aaaaaatgaa tgttaacgat gtttacggta cgagctttca caataaaaat
     1921 aaagaatcac gcatagtatc cattagcggt acaagttttg taccaggtaa aacagctata
     1981 gtattcctta tgccaatagg gaatgatatg acag
//
LOCUS       DAPGRN010000258         1950 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPGRN010000258 DAPGRN010000000
VERSION     DAPGRN010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.263"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1278..1880
                     /gene="fimB"
                     /locus_tag="RHW33_004768"
     CDS             1278..1880
                     /gene="fimB"
                     /locus_tag="RHW33_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV1648176.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIYRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          591 a          351 c          404 g          604 t
ORIGIN      
        1 acagagatcc agattaaata cctgagtata aaatctcttc tgatgtttaa ttgatttgaa
       61 tgttcgtaag ctatatcact tactcaatcc attttaccca gagtcataaa atagagtgtc
      121 tgttgatctc attttcatat tcctttcaga ctgaagtgcg ttttcatcac ccgatgagaa
      181 agaactgaag cgatgtttgc catagattgc aagatgccct gtctcattgt ctatcgtggt
      241 gcacctttat acctgttata ccagatcaaa aatcacgcaa tccatacaac aaaaccagat
      301 ttgcaattcg tgtcacaaaa tatgtcgatc tttttctaag aagaagatgc catatgaagc
      361 caaacgaaca cttgcggagg ccttcacaaa ccaaagatat ttgtttaatt atctgtttta
      421 tatgtgattt atttagagtc taaacaaggg aggctttgca agctaactta gtgggcgtgg
      481 tgaaaataag tatttacccg ccatcaggct gagcataatt ctcatcatga aatatgtttc
      541 atggtttgtg gcttgtaagt ggtcactcct gaagtcgatc tggagaggct tgttgatgtt
      601 ggtgttttca ggatgatgtt tcacttagtt tgtttgccgt atcgcccggc gaatggctgt
      661 gattgaggaa ggtttaagtc gtagtgacca aagctatatt taccaacgaa tgtagatgaa
      721 aaaatcgttt cctgctttcc cccatatctc catgataaaa aggaatgtaa caatctcatg
      781 gcctaaactg acgaaccagc caggaataat cgctagggac ctaagaatta gcatgataat
      841 agccactaag aaattactgc gttccatgaa atagccattt tgtggcaaat ggagttgact
      901 aataatgtca tatgtgagac ggctagttga acgaatatta aattttgctg aattttttat
      961 gctgatttta cttgttacag aacatatcac atgatatata gataagatta gttgcattaa
     1021 tgatgagggt tattattaga ttcgtatccg attgataaat atataaaggt acatagcatg
     1081 caagagcata gcgtttgtat ggcaacgtta ttataattaa ctgttgctac cccatttaag
     1141 ttcactcaga agaactggtc cacttacgtt agttattaag caaacgttcg cttttataaa
     1201 cataatcagg ataaaaatgt tggattattg ctaacccagc acagctagtg cgcgtctgta
     1261 attataaggg aaaaacgatg aagaataagg ctgataacaa aaaaaggaac ttcctgaccc
     1321 atagtgaaat cgaatcactc cttaaagcag caaataccgg gcctcatgca gcacgtaatt
     1381 attgtctgac tttgctttgt tttattcatg gtttccgggc gagtgaaatt tgtcgattga
     1441 ggatttcgga tattgatctt aaggcaaagt gtatatatat ctatcgatta aaaaaaggct
     1501 tttcaacaac gcacccgcta ttgaataaag aagttcaggc tttaaaaaac tggttgagta
     1561 tccgtacttc gtacccgcat gctgagagcg agtgggtatt tttatcgcgt aaggggaatc
     1621 cgctttctcg gcaacagttt taccatatta tctcgacttc cggtggtaat gccgggttgt
     1681 cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg gcgaatatgg
     1741 gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt catactgtct
     1801 ggtataccgc cagcaatgca gggcgttttt acggcatctg ggatagagcc agaggacgac
     1861 agcgtcacgc tgttttatag agatagttga gataccaggg atggtgttaa aaactacatt
     1921 attcttacga tatatcaaaa atgattaacc
//
LOCUS       DAPGRN010000259         1945 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPGRN010000259 DAPGRN010000000
VERSION     DAPGRN010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.264"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..193
                     /locus_tag="RHW33_004769"
     CDS             <1..193
                     /locus_tag="RHW33_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV1648177.1"
                     /translation="NGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTP
                     PAEAEKAYYASIGNDDLAA"
     gene            complement(234..955)
                     /locus_tag="RHW33_004770"
                     /pseudo
     CDS             complement(234..955)
                     /locus_tag="RHW33_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(950..1271)
                     /locus_tag="RHW33_004771"
                     /pseudo
     CDS             complement(950..1271)
                     /locus_tag="RHW33_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1436..>1945)
                     /locus_tag="RHW33_004772"
     CDS             complement(1436..>1945)
                     /locus_tag="RHW33_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV1648178.1"
                     /translation="SGDTIAVIEAPGASNARINGLGGGETNYFGYALASYLEPYRENR
                     ISMDPLSLPSDISLDFTSTSVIPTKGAFIPAKFKTNRGKKVLLNLEDEYGNPIPFGAI
                     VRVVSDSDNMSIVGDDGVVYLTAMKDTGVIEVVWGDRNNQKSRKIGYSLNDTPTPAGV
                     YFAKTAIIK"
BASE COUNT          501 a          461 c          410 g          573 t
ORIGIN      
        1 caatggtctt tacaaagcgg aggtaataca ccgtaagagc tggaaaaacc gtgcagaagt
       61 ggaactggcc acactcacgt gggtggactg gtataacaat cgacgattgc tggaaaggct
      121 gggccatact cctccggcag aagcagaaaa agcttattat gcttccatcg gaaacgatga
      181 tctggcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg gttcagaacg
      241 acctccccct tatttccggt gaaaagaagg ttttttcccg cattcaggtc atttcctttt
      301 ttgccgtgca tatcccagct tgtatttccc tgggtcaggg tgccggtacc attagcagca
      361 tcatatttcc actgcagtgc ttccgtacca gatgtgtagg ttatggtttt gtcaaaatca
      421 ttttgcggct gttgaccgag gaagtcctga gtggtgacga cccagttatt gcctgaagag
      481 gtcattgaag atgtaacacc aatcaacacc atttttttcc ccaggaatca taagcaaata
      541 gtggagagcc actatccccc ataattagat agtttgctaa aggcccctgc gatgggttaa
      601 aaatattagc gcttggaatg gtaatcatct gcccgttatt ataggagttt agtgcaccaa
      661 ctgtaccacc gaccaaaaaa acaccatttg tataaatctg tgtgaggtta ccgtttttgt
      721 ccttgataaa ctgctgtccg ccaccaagac gatagaatgc cgtaaaacgg ctttctgcct
      781 gataggctcc gctcaccgcc cccacatcag acacttcagt cggagcaacc tctgtcacca
      841 gtttactcag gcgacggttt tttatatcaa gaccactatt attgtttgtg ccaactgcag
      901 tataagtgtt tctggcgtac ccgaagctca tcgtatcgga gccgttaaca tgtttttcaa
      961 taaaaacata cttcattaca ggcgcttcgc gaagtatgtc gctgcctttt ggaggatggc
     1021 cagctcctct acctgctccg ccagttgccg cttcaggcgg gcattttcag cggccagctc
     1081 actttcacgc tatggcgagg ctatttattg ctgcagtttg ctgcgccacg catagagctg
     1141 agattcatac aggctgagtt cacggactgc ggcagcaata ccaatgcgtt cagcaagctt
     1201 cagggcttca ctgcgaaatt caggcgaatg ctgtttacgt gttttttgct ggtttatact
     1261 gtttttgtca tgtgagtcac ctttgactga aagtttactc acttagccgc gtgtccacta
     1321 ttgcttggta agatcacgtc tagattttta tcaatgagtt gctgtacgat tgactgataa
     1381 gattaccgca ctaatcatca gtgtggtaat cttatacact ctatccttat ttatattatt
     1441 taattatggc tgttttagca aaatagaccc cagcaggggt gggagtatca ttcaggctat
     1501 agccaatctt tcggcttttc tggttatttc tatctcccca gacaacctca ataacgccag
     1561 tgtctttcat tgcagttaga tatacaactc catcatcacc gacaatactc atattgtcac
     1621 tatcactaac gactcgaacg atcgcaccaa atggaatggg gtttccatat tcatcttcaa
     1681 ggttgagcaa aacctttttt cctctatttg ttttgaattt tgctggtatg aaagccccct
     1741 ttgtcggaat aactgatgta ctggtaaaat ccagtgagat gtcagagggt aaagataacg
     1801 ggtccattga tattctattt tctcggtatg gttccaaata actagccaat gcataaccaa
     1861 agtaatttgt ctcaccacca cctaaaccat ttattcttgc atttgatgct cctggagctt
     1921 ctatcactgc aatagtatcc ccaga
//
LOCUS       DAPGRN010000260         1944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPGRN010000260 DAPGRN010000000
VERSION     DAPGRN010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.265"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            130..384
                     /gene="bssS"
                     /locus_tag="RHW33_004773"
     CDS             130..384
                     /gene="bssS"
                     /locus_tag="RHW33_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV1648179.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            499..1617
                     /gene="solA"
                     /locus_tag="RHW33_004774"
     CDS             499..1617
                     /gene="solA"
                     /locus_tag="RHW33_004774"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV1648180.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDDLSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1665..1778
                     /locus_tag="RHW33_004775"
     CDS             1665..1778
                     /locus_tag="RHW33_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV1648181.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          510 a          464 c          450 g          520 t
ORIGIN      
        1 aaatttatct gtttccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga
       61 agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag
      121 ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac
      181 atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat
      241 aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac tgatgttgcc
      301 agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttcccg
      361 gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg
      421 gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat
      481 gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggg
      541 gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgcttatgac cgacgcccat
      601 atgccaccac atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat
      661 ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcac aaacgctgtg ggatgacctc
      721 tcccgccaca acgaagatga tcctattttt gtacgctctg gtgtcattaa ccttggcccg
      781 gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt
      841 gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac
      901 tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc
      961 tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca
     1021 attcgtcatg acgatgatgg cgtaactatt gaaacggctg acggtgagta tcaggcgaaa
     1081 aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct gcctgtccag
     1141 cccgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa
     1201 ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa
     1261 aacgacgcgt tgaagattgg taaacataac ggaggccagg ttatccattc agcggatgaa
     1321 cgtgttccgt ttgcggaagt ggccagtgat gggtcagaag ccttcccgtt cttgcgcaat
     1381 gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct
     1441 gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct cattaccggc
     1501 ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg
     1561 caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca
     1621 tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc
     1681 actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc tggggattgt
     1741 tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg acataaactg
     1801 acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgagatta ttcctgcttc
     1861 tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag attctttcat
     1921 tattaattca aaattattga aata
//
LOCUS       DAPGRN010000261         1925 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPGRN010000261 DAPGRN010000000
VERSION     DAPGRN010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.266"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..123
                     /gene="psiB"
                     /locus_tag="RHW33_004776"
     CDS             <1..123
                     /gene="psiB"
                     /locus_tag="RHW33_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HDV1648182.1"
                     /translation="QRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            120..839
                     /locus_tag="RHW33_004777"
     CDS             120..839
                     /locus_tag="RHW33_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HDV1648183.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(860..1039)
                     /locus_tag="RHW33_004778"
     CDS             complement(860..1039)
                     /locus_tag="RHW33_004778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein sok"
                     /protein_id="HDV1648184.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQAVYG"
     gene            1119..1277
                     /locus_tag="RHW33_004779"
     CDS             1119..1277
                     /locus_tag="RHW33_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1648185.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            complement(1515..1892)
                     /locus_tag="RHW33_004780"
                     /pseudo
     CDS             complement(1515..1892)
                     /locus_tag="RHW33_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          371 a          537 c          584 g          433 t
ORIGIN      
        1 cagcggcggt ttgcaccgga agccgtcagc cattcactgg cactggcggc gtcactggac
       61 gcgcaggggt acagcgtcag tgacatcatc catatcctga tggcggaagg aggtcaggca
      121 tgagcgcacg ttcacaggca ctggttcccc tcagcgcaga gcaacaggcc gcatggcggg
      181 cggtggcgga gacagaaaaa cgccgccacc agggaaacac gctggcggaa tatccgtatg
      241 cgggggcgtt tttccgctgt ctgaacggaa gccgcaggat atcgctgtct gacctgcgat
      301 ttttcatgcc gtccctgacc gcagaagaac tgcgcggcaa ccgttctcag tggctgtatg
      361 ccgtcgatgt gctgattgaa actcagggcg aggtctgcct tcttcccctt cccggcgatg
      421 cagcagagcg gctgttcccg tcggtgcggt tccgtgtcag ggagcgcagt cgccacaaat
      481 ccgcactggt catgcaaaag tacagccgcc agcaggcgag ggaggcagag cagaaagccc
      541 gtgcgtacca ggcgctggtc gctcaggctg aaatcgaact ggcattccat tcgccggaaa
      601 cagtcggaag ctggcatgca cgctggagtg accgggtggc agagcatgac ctggaaaccc
      661 tgttctggca gtggggtgag cgttttccgt cactggccgg aatggaacgc tggcagtggc
      721 aggatatgcc gttctggcag gttattgcgg aagccagtct tgcagcaaga gaggccggtc
      781 acgctgtccg tgagatggag cggtggatgg tgccgaacaa actccgggag gcggcgtgat
      841 gcggcaacaa tcacacagat tacccgtaaa cagcctgaat gagcgggtta ttttcaggaa
      901 aagtgagtgt ggtcagcgtg caggtatatg ggctatgatg tgcccggcgc ttgaggcttt
      961 ctgcctcatg acgtgaaggt ggtttgttac cgtgttgtgt ggcagaaaga agatagcccc
     1021 gtagtaagtt aattttcatt aaccaccacg aggcatccct atgtctagtc cacatcagga
     1081 tagcctctta ccgcgctttg cgcaaggaga agaaggccat gaaactacca cgaagttccc
     1141 ttgtctggtg tgtgttgatc gtgtgtctca cactgttgat attcacttat ctgacacgaa
     1201 aatcgctgtg cgagattcgt tacagagacg gacacaggga ggtggcggct ttcatggctt
     1261 acgaatccgg taagtagcaa cctggaggcg ggcgcaggcc cgccttttca gggctgatgc
     1321 tggtctgact gcactgaagc gcctttataa aggggctgct ggttcgccgg tggccccttt
     1381 ctccttgctg gcattgtacg ggcatgaaca accagactgc caggaaggcc gtttccctcc
     1441 gggaacgggg atgaacgtgc gcctgcggcg cccgttcttt tcccccgcct tctctgttta
     1501 tgacttacca gaaatcacat caccttccct ttgattattc tctttttcct cctgctctga
     1561 ctctgattac tgctctgact ctgattactg ctgccctgat tattgtctgt tttgttgttc
     1621 tcctgcttat gccttttccg ggcgtccccc tggcggggcg ggctgtcgtg aacggagcca
     1681 gcgaagctgt ctccgccctg gcgggcttcc atccctgacg caaaaacccg ggtttgcagc
     1741 gaccggaacg gtcgcgccac ccttgtcgcg ccgtggcctg cgctcccggc atcccggcaa
     1801 aagacgccgg gatgtaagga gcaacgccgt ttcgcaaacc gcgcttcacg tcgttgcggc
     1861 gggtccctgc ccgttgttct gtccggtgcc attcacctgt ttaagcccct gtcgccgggg
     1921 gcata
//
LOCUS       DAPGRN010000262         1881 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPGRN010000262 DAPGRN010000000
VERSION     DAPGRN010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.267"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..491
                     /locus_tag="RHW33_004781"
     CDS             15..491
                     /locus_tag="RHW33_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV1648186.1"
                     /translation="MPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRG
                     EFIRGWDDGRSADAGRTILSAQGDAIRNIYGEFRTVNTENYSIWETAGSFKGAIVPLS
                     PSTNNSYFSLTRSMVTERADGAVYPKVIGLDASRIVPTANENRPRNIAFNYIVRAA"
     gene            491..1093
                     /locus_tag="RHW33_004782"
     CDS             491..1093
                     /locus_tag="RHW33_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV1648187.1"
                     /translation="MNKAVLNNELIAIKAGDITIYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPVTHKAGYAICRSADFNSWEYVPDHRGEVVYNTETGESKEITAPGDYPEK
                     TTTIAPLTPYDKWDGEKWVTDTEAQHRAAVDMAETQRQSLIDAAMVSISLIQLKLRAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(1065..1508)
                     /locus_tag="RHW33_004783"
     CDS             complement(1065..1508)
                     /locus_tag="RHW33_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDV1648188.1"
                     /translation="MNEYVYSARHNAFFPVDMIDKYKSEGWDLSDAKEVNQNIISEFM
                     AEPPQGKIRIAGDDGLPAWADIPLPTYEELIEITESERQLLINQANEYMNSKQWPGKA
                     AIGRLKDDELAQYNLWLDYLDALEMVDTSGAPDIEWPTPPAVQAR"
     gene            complement(1529..>1881)
                     /locus_tag="RHW33_004784"
     CDS             complement(1529..>1881)
                     /locus_tag="RHW33_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV1648189.1"
                     /translation="RDVGTGENQIPDMASFASGDGWMKLPNGKILQYGRGAVTPTLST
                     QTMRITFSIPFPKKADCAMLTHSGDGGAPLGAGRGFVMTAEGPTLTGFNSAYRTSSTS
                     DTVSMNYSWWAVGE"
BASE COUNT          511 a          449 c          431 g          490 t
ORIGIN      
        1 cattacctgt cggtgtgcct gttccgtggc cttccgccac tccgccgaca ggctggctga
       61 aatgcaatgg tgcggctttt tctgctgaag aatacccgga actggcaaag gcttacccga
      121 caaataaatt gcctgattta cgcggtgaat ttattcgtgg ctgggatgat gggcgtagtg
      181 cggatgcggg gagaacaata ttatcagctc agggcgatgc catacgtaat atctatggtg
      241 agttcagaac tgtaaacact gaaaattatt caatatggga aacagcaggc tcgtttaagg
      301 gggccatagt gccattgagt ccctctacaa acaatagtta tttctcctta accagaagta
      361 tggtgacaga aagagcagac ggcgctgttt acccaaaagt gattggcctt gatgcttcaa
      421 gaattgttcc aactgcaaac gaaaaccgtc ctcgtaacat tgcctttaat tatatcgtga
      481 gggctgcctg atgaataaag cggtattaaa taacgaactc attgctataa aagcgggaga
      541 cattaccatt tataattatg atggtgaaac gcgggaatat atttccacat caactgaata
      601 tcttgcggtt ggcgtcggta tcccggcatg ttcttgttta gatgcgccag ttacacataa
      661 agctggttat gcaatctgcc gttctgcaga ttttaactca tgggaatatg tgccagacca
      721 tcgcggtgaa gttgtctata acaccgaaac gggagaatca aaagaaatca cagctccggg
      781 tgattaccct gaaaagacaa ccactatcgc cccgttaacg ccatacgata aatgggatgg
      841 tgagaaatgg gtgacggata ccgaggcaca gcatagagca gcagtagaca tggcagaaac
      901 acagcggcag tcgctgattg atgctgcaat ggtttccatt agtctgattc aactgaaatt
      961 gcgggccgga cgtaaactga cgcaggcaga aacaacccgg cttaacgctg tgctggatta
     1021 cattgacgcg gtgacggcaa cagataccag caccgcgccg gatgtcatct ggcctgaact
     1081 gccggaggcg taggccattc aatatctggc gcaccggaag tatcgaccat ttccagtgcg
     1141 tccagataat ccagccacaa attatattgc gccagttcat catctttcag acgaccaata
     1201 gcggctttac cgggccattg cttactgttc atgtattcgt tggcctggtt aattagcagc
     1261 tgtctttctg attcagtaat ttcaataagt tcttcatacg tgggtagagg aatatctgcc
     1321 cacgcaggca gcccatcatc tccggcaata cggatttttc cttgtggcgg ttcagccata
     1381 aactcactga taatattttg atttacttcc ttagcgtctg ataaatccca tccctctgat
     1441 ttatatttat caatcatatc cacagggaaa aaagcattat gccttgcgct ataaacatat
     1501 tcgttcatat aaatcaccct gaataaaatt actcaccaac agcccaccaa ctgtaattca
     1561 tcgataccgt gtcactggtt gatgacgttc tgtaagcaga attaaagccg gttaacgttg
     1621 ggccttctgc agtcatcacg aaccctcgcc cagcgcctaa aggcgcaccg ccatcaccag
     1681 aatgagtaag catggcgcag tccgcttttt tggggaaagg gatgctgaat gtaattctca
     1741 ttgtttgcgt cgataatgtc ggcgtaaccg caccacgacc atattgcagg attttcccgt
     1801 tgggtaattt catccatcca tcaccactgg caaaagaggc catgtccggt atctgatttt
     1861 cccctgtccc cacatcccgc t
//
LOCUS       DAPGRN010000263         1861 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPGRN010000263 DAPGRN010000000
VERSION     DAPGRN010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.268"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            210..713
                     /locus_tag="RHW33_004785"
     CDS             210..713
                     /locus_tag="RHW33_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV1648190.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(792..1043)
                     /locus_tag="RHW33_004786"
     CDS             complement(792..1043)
                     /locus_tag="RHW33_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV1648191.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1158..1244)
                     /gene="azuC"
                     /locus_tag="RHW33_004787"
     CDS             complement(1158..1244)
                     /gene="azuC"
                     /locus_tag="RHW33_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV1648192.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1507..1830
                     /locus_tag="RHW33_004788"
     CDS             1507..1830
                     /locus_tag="RHW33_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV1648193.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          485 a          437 c          408 g          531 t
ORIGIN      
        1 tttcatcaat gaaatgtaaa aatatataaa cttgatgatt taagcatttt cttatacccg
       61 ttcagacgtt attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga
      121 ccggcagaaa ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct
      181 ctccacttct ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca
      241 actctcaaat gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt
      301 gttctgaaca gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg
      361 tgacccaaat gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta
      421 aagccattga tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga
      481 tggaagaata cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac
      541 tgaatgatga ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg
      601 gcctgttgct gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc
      661 cagtgcagac cgaccaacat gttctgaatg tcgtgtcgca tcagctgcat tgatcataca
      721 tcggcgctaa tgcattgcgc cgatgaagtt tttgagaaac cgctgcctca tctttttgaa
      781 gcagcggttt tttaatggga ttcgccttgt ggggtaaact tgagttcaat cagcgcgatg
      841 gctttttgga ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg
      901 atattcttca gctggctgga cattttttcg cgaacttcaa cgggcgcgat tacatcgaga
      961 acatccagaa tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga
     1021 tcggcattca gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt
     1081 agcggcttca catcttcaac gatagtcaca tccaccctca tcaggacaca aaaaaacctg
     1141 ccggagcagg ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg
     1201 caatagttat tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca
     1261 tttgttatac aggtgttatt atctttgcgc ttataatatg acaaccatca caaaaatcaa
     1321 tctttatgtg atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaag
     1381 aacagccccc ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt
     1441 ttccggccat tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc
     1501 tggaccatga gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt
     1561 actcgccagg cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat
     1621 gaccaggctt ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat
     1681 cgcgcatgcc agcaggaagg ctatacccac gcgatcccct ttggtcagcc agtaggcaac
     1741 tgtagccttt ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc
     1801 catcattccg ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg
     1861 a
//
LOCUS       DAPGRN010000264         1842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPGRN010000264 DAPGRN010000000
VERSION     DAPGRN010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.269"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1842
                     /gene="traG"
                     /locus_tag="RHW33_004789"
     CDS             <1..>1842
                     /gene="traG"
                     /locus_tag="RHW33_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HDV1648194.1"
                     /translation="PLRGVYDSNNNFVTCKDASVSLKDKLNLDTQSGGKTWHYYAQQL
                     FGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNVTINALKEGITSYAARNGDSAS
                     LVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTGIAIGIFPLLVLAAVFNKLTLS
                     VLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPVVLSEISQIQLKYSDLASTAGY
                     LSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTASAAGSVVDGNYSYGNMQTENVN
                     GFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDASGAMSRLPVGINATRQIAAAQ
                     QEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRGSSDSVTGGADSTMSAQDSMMA
                     SRMRSAVESYAKAHNISNEQATRELASRSTRGSAGMYGDAHAEWGVKPKILGVGGGVG
                     VRGGGRASIDWSDEDAHQASSGSRASHDARHDIDARATQDFKEASDYFTSRKVSESGS
                     HTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQ
                     FAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQPGVDNA"
BASE COUNT          446 a          454 c          537 g          405 t
ORIGIN      
        1 ccgctacggg gcgtttatga cagcaataat aatttcgtga cctgtaagga cgcgtcggtt
       61 tcgctgaaag acaagctgaa tctggataca cagagtggcg gaaaaacctg gcattattat
      121 gcccagcagc tatttggtgg acgccctgat ccgaacctgc tgttcagtac gctgattggc
      181 gacagttaca gttacttcta tggctcaagt aaatcagcca gccagatcat ccgccagaat
      241 gtcaccatca atgcgctgaa ggaggggatc accagttatg ccgcgcgtaa tggtgattca
      301 gccagcctgg tgaatctggc caccacgtca tcgatggaga agcaacgtct ggcacatgtc
      361 tccatcggtc atgtggcgat gcggacactg cccatgacgc agaccatcct gacggggatc
      421 gccatcggta ttttcccgct tctggtactg gcggctgtct tcaacaaact gacgctgtcc
      481 gtcctgaaag gctatgtgtt tgccctgatg tggttgcagt catggccaat gttgtacgcc
      541 attctgaaca gcgccatgac attttatgcg aagcagaatg gtgcaccggt ggtgctctcg
      601 gaaatttcac agatacagct gaaatactcc gatctggctt ccactgccgg gtatctttcc
      661 atgatgatcc cgccattatc ctggatgatg gtcaggggac tcggggccgg attttccagt
      721 gtgtacagcc atttcgcttc ctctgctatc agcccgactg ccagtgcggc aggtagtgtg
      781 gttgacggta attactccta cggcaacatg cagacggaaa acgtgaacgg attcagctgg
      841 agcaccaaca gcaccacgtc gtttggtcag atgatgtacc agaccggcag tggcgcaacc
      901 gccacacaga cccgtgacgg taatatggtg atggatgcga gcggggcgat gtcccgttta
      961 ccggtcggta tcaatgcaac gcgtcagatt gcggcggcac aacaggaaat ggcccgggag
     1021 gcgtcgaaca gagcagaaag cgccctgcat gggttcagca gcagtattgc cagtgcctgg
     1081 aactcgctca gtcagtttgg ttctaaccgg gggagcagtg attctgtcac cggtggtgct
     1141 gacagcacga tgagcgcaca ggactccatg atggccagcc gtatgcgcag cgcagtggaa
     1201 agctatgcga aggcgcataa tatcagtaat gagcaggcga caagagaact ggcatcaaga
     1261 agtacgagag gttctgcggg gatgtatggc gatgctcatg ctgaatgggg ggttaaacct
     1321 aaaattcttg gtgttggtgg tggcgtaggt gttagaggtg gtggtcgggc aagtattgac
     1381 tggagtgatg aggatgcgca tcaggccagc agtggttcac gggccagcca tgatgctcgc
     1441 catgatatcg atgccagggc tacacaagac tttaaagagg ccagcgatta ctttaccagt
     1501 cgcaaggtca gtgaatccgg cagccatacg gacaataatg ccgattcccg tgtggaccag
     1561 ctgtctgcag ccctgaactc agcaaaacag agttacgacc agtacacgac gaatatgacc
     1621 cgcagccatg aatatgctga aatggcatcc cgtactgaaa gcatgagtgg gcagatgagt
     1681 gaagacctgt cgcaacagtt tgcacagtat gtgatgaaac acgcgccgca ggatgccgaa
     1741 gctattctga caaacaccag ttccccggag attgcagaac gccgtcgggc tatggcgtgg
     1801 tcttttgttc aggaacaggt gcagcctggt gttgataatg cc
//
LOCUS       DAPGRN010000265         1829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPGRN010000265 DAPGRN010000000
VERSION     DAPGRN010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.270"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..1503)
                     /gene="gltA"
                     /locus_tag="RHW33_004790"
     CDS             complement(220..1503)
                     /gene="gltA"
                     /locus_tag="RHW33_004790"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV1648195.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDVKH"
     gene            complement(1709..1816)
                     /locus_tag="RHW33_004791"
     CDS             complement(1709..1816)
                     /locus_tag="RHW33_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648196.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
BASE COUNT          484 a          414 c          449 g          482 t
ORIGIN      
        1 gtgggtgcta cccaatgtat tctacatcga ttggcgggat aacgtggtgg tgaatgaaaa
       61 attaggattt tatttagtta tcggatatgt attcttgtaa cgtgtaatta tgtatttgtg
      121 tccgattacg tttttttgtc gatagacata actaacggac aaaaaaaagc ccgccagata
      181 ttcaggcggg ctaaacgata tatggttcaa atcagataat taatgtttaa catctgattt
      241 aaagtctcgt ttttcatagc cggtgtacaa ctggcgcgga cgggcgatct tcatgccgtc
      301 actgtgcatt tcgctccagt gagcgatcca gccaacggta cgggccatag cgaagatgac
      361 ggtgaacatg gaagacggaa tacccatcgc tttcaggatg ataccagagt agaaatcgac
      421 gttcgggtac agtttcttct cgataaagta cgggtcgttc agcgcgatgt tttccagctc
      481 catagccact tccagcaggt catctttggt gcccagctct ttcagaactt catggcaggt
      541 ttcacgcatt acggtggcgc gcgggtcgta atttttgtac acgcggtgac cgaagcccat
      601 caggcggaaa gaatcatttt tatctttcgc acgacgaaca aattccggaa tgtgtttaac
      661 ggagctaatt tcttccagca ttttcagcgc cgcttcgtta gcaccaccgt gcgcaggccc
      721 ccacagtgaa gcaatacctg ctgcgataca ggcaaacggg ttcgcacccg aagagccagc
      781 ggtacgcacg gtggaggtag aggcgttctg ttcatggtca gcgtgcagga tcagaatacg
      841 gtccatagca cgttccagaa tcggattaac ttcatacggt tcgcacggcg tggagaacat
      901 catattcagg aagttaccgg cataggagag atcgttgcgc ggataaacaa atggctgacc
      961 aatggaatac ttgtaacaca tcgctgccat ggtcggcatt ttcgacagca ggcggaacgc
     1021 ggcaatttca cgatgacgag gattgttaac atccagcgag tcgtgataga aagccgccag
     1081 cgcgccggta ataccacaca tgactgccat tggatgtgag tcgcgacgga aagcgtggaa
     1141 cagacgggta atctgctcgt ggatcatggt atgacgggtc accgtagttt taaattcgtc
     1201 atactgttcc tgagtcggtt tttcaccatt cagcaggatg taacaaactt ccaggtagtt
     1261 agaatcggtc gccagctgat cgatcgggaa accgcggtgc agcaaaatac cttcatcacc
     1321 atcaataaaa gtaattttag attcgcagga tgcggttgaa gtgaagccag ggtcaaaggt
     1381 gaacacacct tttgaaccga gagtacggat atcaataaca tcttgaccca gcgtgccttt
     1441 cagcacatcc agttcaacag ctgtgtcccc gttgagggtg agttttgctt ttgtatcagc
     1501 catttaaggt ctccttagcg ccttattgcg taagactgcc ggaacttaaa tttgccttcg
     1561 cacatcaacc tggctttacc cgtttgttat ttggctcgcc gctctgtgaa agaggggaaa
     1621 acctgggtac agagctctgg gcgcttgcag gtaaaggatc cattgatgac gaataaatgg
     1681 cgaatcaagt acttagcaat ccgaattatt aaacttgtct accactaata actgtcccga
     1741 atgaattggt caatacttcc acactgttac ataagttaat cttaggtgaa ataccgactt
     1801 cataactttt acgcattata tgcttttcc
//
LOCUS       DAPGRN010000266         1763 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPGRN010000266 DAPGRN010000000
VERSION     DAPGRN010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.271"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..230
                     /locus_tag="RHW33_004792"
     CDS             <1..230
                     /locus_tag="RHW33_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648197.1"
                     /translation="ANQSTQASLRILESTTHAEKDIVEQAKNVMHSVMSSYLDQINRD
                     VQGRDQALNLLQQIIQTIIDTDSAIVQGIRS"
     gene            274..1623
                     /locus_tag="RHW33_004793"
     CDS             274..1623
                     /locus_tag="RHW33_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IpaD/SipD/SspD family type III secretion system
                     needle tip protein"
                     /protein_id="HDV1648198.1"
                     /translation="MINKDNNLNIDILRQQNANIPLKEASSQSEEPHQLVNSKSEGNL
                     HNSASKDNNIGAMLKKDVEKLSTLVSERSPRNEVWKCQHDIQLNFVKNMLRNFPTENL
                     SEKGSALLHESYLLIKNVLQLTENVNTIDGKLKDPAKILTLHNSVRKTLANIADNTTG
                     SSAMAKTKGTLTHAIASLDSSVPEMRSSATSSTLTASSSSEFKSDKYICSDIADLMSV
                     LGSDYLEIYANSVEIMSAYWQDFSEHIQSNMGKWTHSNKKGDAIVFDVNAFQKALMHF
                     YYIDKYPNGDFHYHYNPDYVLYPPAPADKIGVPLEEAEKWCSALGLPVIPPDPKHRTP
                     SPIVEVEPQGSGLYVIIPNPQIIDSMSQSSDSMVHRDDKGKEKNISKEFTGYEISTAE
                     YQAWLAGYNSQAENMKTDVQVITTKYSTANSTYDTIIKLLSSTITALFDSAKDYLRF"
BASE COUNT          609 a          351 c          330 g          473 t
ORIGIN      
        1 ttgctaatca atcaactcaa gcatcactta gaattctaga aagcactact catgcagaaa
       61 aagacattgt tgagcaggct aaaaatgtta tgcattcagt tatgagtagc tatctggatc
      121 aaattaatcg tgatgttcaa ggaagagatc aggcactcaa tttattacag caaataatac
      181 aaaccattat tgatactgat agtgctatcg ttcaaggcat tcgctcataa ataattgcaa
      241 taatagttaa ttatatttaa ggaggaatga cacatgatta ataaagataa taatttaaat
      301 atagatattc tacgccagca gaacgcaaac attcctctta aagaggcatc ctcgcaaagt
      361 gaggagcctc atcaactggt aaacagcaaa agcgagggaa atttgcataa cagcgccagt
      421 aaggataata atattggcgc tatgctcaag aaagatgttg agaaactatc aactttagtc
      481 agtgaacgta gcccgcgaaa tgaagtctgg aaatgccagc atgacataca acttaacttc
      541 gttaaaaaca tgctcagaaa tttcccaact gagaacctgt cagagaaagg atccgcgctc
      601 ttacacgaaa gttatttgct gataaaaaac gttctgcagc ttacagaaaa tgttaatact
      661 attgacggaa agttaaagga tcccgctaaa atattgaccc tgcataactc tgtgagaaaa
      721 accctggcta acattgctga caacacaacc gggtcaagtg caatggcaaa gaccaaagga
      781 accttaactc acgctatcgc ttctttagat agttctgtgc ctgaaatgag aagttcagca
      841 accagcagta ctttaactgc cagtagttca agcgaattta aaagtgataa atatatatgc
      901 agtgatattg ctgatttgat gagtgtgctc ggtagtgact atctcgaaat ctatgcaaat
      961 agcgttgaga taatgagcgc atactggcag gacttcagtg aacatatcca aagcaatatg
     1021 ggaaaatgga ctcactctaa taaaaaaggc gatgcgatag tttttgatgt caatgcattc
     1081 caaaaagcct tgatgcattt ttattatatc gataaatatc ctaatggtga ttttcattat
     1141 cattacaatc cagactatgt tttataccct ccggcacctg ctgacaaaat tggtgttcca
     1201 ctggaagaag cagaaaaatg gtgctccgct ttaggacttc cggtaattcc acccgatccc
     1261 aaacaccgca caccttctcc gattgtcgaa gttgaaccgc aaggtagcgg tctgtatgtt
     1321 attattccca accctcagat tatagacagt atgagtcagt cttcagactc aatggtacac
     1381 agggatgata aagggaagga aaaaaatata tctaaagaat ttaccggcta cgaaataagc
     1441 acggctgaat accaagcatg gctggcgggt tataacagcc aggcagaaaa catgaaaacc
     1501 gacgtgcagg ttatcacgac taaatacagt acagccaact ccacctacga tacaattata
     1561 aaattattat cttcaaccat tactgcgttg tttgattcag cgaaggatta tttacgcttt
     1621 taattaagta acacccacaa ttatcgtcca ctatctggtc ccgtaaaatg ggaccagcgt
     1681 ttcatattat taagcatcag ctttcattca tttcttcatc acaatctcac aaaatgagcg
     1741 ttttttgata gagcaattct gcc
//
LOCUS       DAPGRN010000267         1738 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPGRN010000267 DAPGRN010000000
VERSION     DAPGRN010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.272"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..231
                     /locus_tag="RHW33_004794"
     CDS             73..231
                     /locus_tag="RHW33_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1648199.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
     gene            1241..1438
                     /locus_tag="RHW33_004795"
     CDS             1241..1438
                     /locus_tag="RHW33_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648200.1"
                     /translation="MEFSSLTELKKYRKSHPEKMAFSYSYALSQGVDKQFRHINIAEA
                     DHFKQFLRQIKRAGLDIRAIC"
     gene            1558..>1738
                     /locus_tag="RHW33_004796"
     CDS             1558..>1738
                     /locus_tag="RHW33_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV1648201.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSEHY
                     TYIPTINIINKLRDEG"
BASE COUNT          383 a          471 c          468 g          416 t
ORIGIN      
        1 cacgaggcat ccctatgtct agtccacatc aggatagcct cttaccgcgc tttgcgcaag
       61 gagaagaagg ccatgaaact accacgaagt tcccttgtct ggtgtgtgtt gatcgtgtgt
      121 ctcacactgt tgatattcac ttatctgaca cgaaaatcgc tgtgcgagat tcgttacaga
      181 gacggacaca gggaggtggc ggctttcatg gcttacgaat ccggtaagta gcaacctgga
      241 ggcgggcgca ggcccgcctt ttcaggactg atgctggtct gactactgaa gcgcctttat
      301 aaaggggctg ctggttcgcc ggtagcccct ttctccttgc tgatgttgta cgggcatgaa
      361 caacctgact tcaggaaggt cgtttccctt caggaacggg gatgaacgcg cgcctgcggc
      421 gcccgttctt ttcccccgcc ttctctggtt atggcttgtc agaaatcaca tcaccttccc
      481 tgtgattatt ctctttttct tcctgctctg attctgacta ctgcagtgtt gtcctgtctg
      541 ccggcgcacc ttcggctccc ggctgcctgg cggcatccgg tcgtcaggcc cgactccgac
      601 aggcagctgc tggcgcacct gccagtctcc gccgaacccg ctggcgcggt ttcgggctgt
      661 cgctacggtc cgatgcgtga aaaccccggg cttgcagcga ccgttccggt cgcgccgcct
      721 ctgtcgcgcc gtggcctgcg ctcctggcat cccggcaaaa ggccgccggg atgtaaggcg
      781 caacgccgtt tcgcaaaccg cgcttcacgt cgttgcggcg ggtccctgcc cgttgttgtg
      841 tccggtgcca ttcacctgtt taagcccctg tcgccggggg catagacagc cacaaccgtc
      901 tccgccagtc caggggtgaa atgcacatcc cgcaaaagtt tttgcccaga agcaggcaaa
      961 aacttttgcg gtctgccccc ggacagtcgt ttccggccgt ggcagtgatg ccccatgcgg
     1021 cgaaggttct caaacaggtg atggtgaata ccggagggaa caaaaggcag gtccccgcaa
     1081 aacgtcaaaa ccgcggctcc cgaaactcaa cagatgatgg gggctgaaaa ccagatagag
     1141 gagaaagcag gatgagtacc cgaaatatcc acgttaacac cgcgtcgtac acgcttcttg
     1201 ttgccgggaa gaaaaaaaca ccggggaaga gtgggacgtc ctggaattca gcagcctgac
     1261 tgagctgaaa aaatatcgca aaagccaccc ggaaaagatg gccttcagtt atagctacgc
     1321 gctcagtcag ggtgtggata agcagttccg ccatatcaac attgcggaag ccgatcactt
     1381 caaacagttc ctgcgtcaga ttaagcgtgc cggcctcgat atccgggcga tctgctgacc
     1441 ggatgtgatg aatatccctc ctgattgttg caatcttgtt aatagagatc ggggggggat
     1501 gatattaagc ggaaaaattt tttattaaat ttattctttc ataaaggagt agctgttatg
     1561 cgattagctt cccgttttgg tcggtataat tccatccgcc gtgaacgtcc tttaacggat
     1621 gatgaattaa tgcagttcgt gccttcggta ttttccggtg ataaacatga gtcccggagt
     1681 gaacattata cgtatattcc aacaatcaat atcatcaata agttacgtga tgaaggtt
//
LOCUS       DAPGRN010000268         1719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPGRN010000268 DAPGRN010000000
VERSION     DAPGRN010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.273"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1719
                     /locus_tag="RHW33_004797"
     CDS             <1..>1719
                     /locus_tag="RHW33_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HDV1648202.1"
                     /translation="YRELNKAAQRSITAPDRIGEGKTWVYQRDTMVERGDNSGVHQYG
                     PAEHFTHIISDKPSPKDKYVAYAINIPDYELAADVYNINVTSPSGQQETFKILINPEH
                     LRQTLERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQI
                     PPVTTFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDII
                     PDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIESGIPGYWQGQVLIPFTIDPVTKER
                     KQIHPEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQY
                     VREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHEKAVMNY
                     MEYLVTCQLDEEQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISS
                     IPSKRRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLK
                     STTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVL
                     AFSRGDS"
BASE COUNT          587 a          343 c          331 g          458 t
ORIGIN      
        1 ttaccgtgaa ctgaataaag ctgcacagcg tagtattaca gcgccagaca ggataggaga
       61 agggaaaaca tgggtttatc aacgagatac aatggttgaa agaggtgata acagcggtgt
      121 tcatcagtat ggtcctgctg aacatttcac ccacattata tctgacaaac cctccccaaa
      181 agataaatat gttgcatatg ctattaacat tcctgactat gagctggcag ccgatgtata
      241 taatattaac gtgacgtcac cttccggaca gcaagaaaca tttaaaatat taatcaatcc
      301 agaacatcta cggcaaacac ttgagcgtaa atctcttact gctgttcaga aatcgcaatg
      361 tgaaatcatc acccccaaaa aacctggcga agcgattctt catgctttta atgccaccta
      421 ccagcaaatc agggaaaata tgtctgagtt tgcacgttgc cattatgggt atatacaaat
      481 ccctccagtg acaactttcc gcgccgacgg accagaaact cccgaagaag aaaaaggtta
      541 ctggtttcac gcttatcaac ccgaagatct ttgtaccatc cataatccaa tgggagattt
      601 gcaggatttt atcgcattgg ttaaagatgc taaaaaattt ggtatcgata tcattcctga
      661 ttataccttt aactttatgg gaatcggggg tagtggtaaa aatgacctgg attatccctc
      721 tgctgatata cgagcgaaga tcagtaaaga tatagaaagt ggtatccctg gctattggca
      781 aggtcaggtt ttgattccat ttactataga tccagtaaca aaagaacgta aacaaatcca
      841 tccagaagat atacatctca ctgcaaaaga cttcgaagca agtaaagata atatttctaa
      901 ggatgaatgg gaaaacctcc atgcattaaa agaaaagcgt ttaaatggaa tgcctaaaac
      961 aacccccaaa agtgaccagg ttattatgct gcaaaatcaa tacgttcgtg aaatgcgaaa
     1021 atatggcgta cgaggtttac gttacgatgc agcaaaacac tcaaaacatg aacaaataga
     1081 aagatcaata accccaccgc ttaaaaatta taatgagcga ttacacaata ctaacttatt
     1141 taacccaaaa tatcatgaaa aagccgttat gaattacatg gaatatctgg taacttgtca
     1201 gttggatgaa gaacaaatgt catcgctgct ttatgaaaga gatgatttaa gcgccattga
     1261 tttttcattg ctcatgaaga cgataaaagc tttttcattt ggtggcgatc tccaaaccct
     1321 tgcatcaaaa ccgggttcca caatctcaag catcccatca aaaagacgga tattgattaa
     1381 cattaaccac gattttccta ataatggcaa tcttttcaat gactttctat ttaaccatca
     1441 acaagatgaa caattagcaa tggcatatat ggccgctctc ccgttcagca ggcctttagt
     1501 ttactgggat ggccaagtat taaaatcaac gactgaaatt aaaaattatg atgggtcgac
     1561 gcgtgtcggc ggtgaggcgt ggcttaataa aggttgctct acctatcagc agctctacaa
     1621 tgaattccac gcattatata tagataaagc aggaatatgg agtgcatttg agggtgtatt
     1681 tgcaactaaa aacgttctgg cctttagtcg tggggattc
//
LOCUS       DAPGRN010000269         1666 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPGRN010000269 DAPGRN010000000
VERSION     DAPGRN010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.274"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1187)
                     /gene="tssI"
                     /locus_tag="RHW33_004798"
     CDS             complement(<1..1187)
                     /gene="tssI"
                     /locus_tag="RHW33_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV1648203.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDR"
BASE COUNT          405 a          449 c          415 g          397 t
ORIGIN      
        1 cggtcccagt taaatttcac ccgcacgcgg ccatgttcat cacagaagat ttcctcgcct
       61 gccggtcccg tcacgacggc gctctgcggg ccgtccacca gcggtttcag caacggctgt
      121 ggccgccatg tccggtctgc cgggattacc gcaaagtggt tattcagcgt ggtacctgaa
      181 ccactgcgtc ccggcaccgc ctgcggctgc tcaccgtgca gttcacttgc caccacctgc
      241 cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg tcccggccat
      301 atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca gccgtccatc
      361 tgccagcggg caaagttctg cccgtgggcg cccttgaaac gtccgggata gtcatacact
      421 tcatactgtg tgcgctggta atcctggtgc tggccttcct ggtcaaaacg tccggcccag
      481 ccggggcgtt taaaggtgta gtctttggtc accacggaag aagggcggat ttgtgcgctg
      541 tagcgaaact ggctgatgca gagggtgctt acctcggtac gggtgttcgg attccagggg
      601 atctcaaagg actccggcag ataacgcacg gtatcgcaca ggaccaggct ttggtcggta
      661 cttttttgcg cgtgctcctc ataaaagaag atgccttcct ccgccgccat ccggcacagg
      721 aaatcgtaat cggtttcgcc gtactggaca caaaactcac gggaaggatg cggctcgctg
      781 aacagcggac tccactcggt caccccgttt tcctgcaata tcgtagcgag gatgctttcg
      841 atgtcctcat tctggaagat acggaagttc tggcgcagcc ctgtgcgcca cagcggcggg
      901 cacaccttca tgctgtacag catctggttt ttgtcgttct cccccagttc aaaccaggtc
      961 accacacctt tcacccggcg ctgtacgtca tcgccctgcc ataccgtcag gtaggccatt
     1021 ttgtccagca cctgcgcaaa ttcaagggaa agaaactgct gactgaccag agaaaggtcg
     1081 agggaaaaaa gtgaagagag tgactggttc agatgaaagg agaccaccgc aaacgcgtcc
     1141 ggtggcaggc cgtccacttc cagcgtgaaa cgtaatccgg ttgacatagc aacctccgtg
     1201 aaaatgaata ataatccgca catattgcat gtacaacatg tcgggcggat gaaataagtg
     1261 agtcgacaac tggcgttacc ggggaatatc agaaaatgtc atggttatgt gatgatttag
     1321 tctgggattt tacttcagag aaacgggcgt ctgtttagtc aacaaaataa tcacaaacgc
     1381 aacggatggg ttctggacgt tattcacgac aggctcgtac attacagcga gtaataaaaa
     1441 acctgatatg caaagttaaa ccaatattca taatgtcttt ttattcacaa caataaataa
     1501 tttaataaca atcattgaaa tgaatgatga tttgtataag gaatatacgc taccgcccca
     1561 ccctgataac atgatctgca cattgccatc ttaacgtcgt taaacactgg cttaatagtg
     1621 gatatttcaa attaggaatt tattaaattc acctgatgaa tatcat
//
LOCUS       DAPGRN010000270         1641 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.276, whole genome shotgun sequence.
ACCESSION   DAPGRN010000270 DAPGRN010000000
VERSION     DAPGRN010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.276"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..439)
                     /locus_tag="RHW33_004799"
     CDS             complement(<1..439)
                     /locus_tag="RHW33_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV1648204.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTVSVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGIGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIIFFAL"
     gene            complement(448..1215)
                     /gene="sctT"
                     /locus_tag="RHW33_004800"
     CDS             complement(448..1215)
                     /gene="sctT"
                     /locus_tag="RHW33_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV1648205.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELSNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1217..1477)
                     /locus_tag="RHW33_004801"
     CDS             complement(1217..1477)
                     /locus_tag="RHW33_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1648206.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1487..>1641)
                     /locus_tag="RHW33_004802"
     CDS             complement(1487..>1641)
                     /locus_tag="RHW33_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1648207.1"
                     /translation="SSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYF
                     DLSINP"
BASE COUNT          571 a          327 c          284 g          459 t
ORIGIN      
        1 ccaatgcaaa aaaaataata taaagaattg ctttaacaaa ttcttttacg gtttttaagc
       61 caaatatacg ctttaagcca tttactggat tcaatgaatc aaacttaaat ttaacagctt
      121 cagttgctaa ttgcatttta ctttgaaacc aggagacaag tattgttgtg aacatgcaaa
      181 ccagcaaaaa aggaatgatc gttttcaacc aaccaatccc catagcttta aaatagtccc
      241 aaatatctgg tttagcgtta tgatcaagga tgtattcaag aatcgacata atgtgatgca
      301 catcaaaaac atatccgaga tataaagtac ccacaagcat tattacagag acggttaagt
      361 ctctactttt tagaatttgc ccttttttag aagcatcctg gagtttcttt tgtgttggct
      421 tttcagtttt attggccatg aaacactcta tttctcaatg tatagttgca attgttctgg
      481 gaaaaaggat aacggcatta ctcgttcagc cagtattggg ccaaaataaa taattaatat
      541 taaaaatgcc agcgcacttt tgacagtcag tgaaatggca aaagcattta gttgtgaagc
      601 ataacgtgcc agcaatccca ggacagcctc accgcccagc ataacggcaa tcacggggct
      661 tgcgtaaacg atagtatgag tcattatgtt attaatatat gagaataaag gctctaattt
      721 aggaaaatta aaattacctg aaggccataa attataactt tgccaaagag tttccagaat
      781 aaagttcatt ccaccgttag taagataaac cgcagcagag aaaaggttaa ataatcttgc
      841 taattccgag gtatcgacac ctgtagccgg atccaaagta ctacttaatg ttgcaccgcg
      901 ttggttatcg ataaagctac ctatagcaag gaaaatccaa aacggggatg ctaataagca
      961 tgcaataaac agccctatga taatttctct ggcagcgaac aaagcaatat cgatttcatt
     1021 taagttcgat aattcatagt ggtaatgctg aactaatgca catgatacca ctattattat
     1081 tggatgacgg actactgttg gtatattccc actggcgaaa aatggtaaca aatagaaagt
     1141 gggtgccaat cggcaaaaac cgagcgctgt agcagccagc agactatgca actgatagag
     1201 tatcgcctca cccatgctat cctctcgcga atgctaaatt cagcatttca ataccaaaag
     1261 aatataattt ttcgccatac catccgctca ttaaaaaaaa gcaaatactt acacataata
     1321 atttcacgcc aaatggcaac gtctgttctt gcaactgtgt taccgtttga aaaagcccta
     1381 ctaataaacc aacaaatgtc gcaactgcga taggtcctgc agacataact aaaatcagat
     1441 acagcgccct attccctgca aaaacaatat cgtccatatt ttttcctcag ggattaatgg
     1501 ataggtcaaa atattgtaaa atgagtcctt ttgataacat tgtccaaccg tccatagcga
     1561 caaacagaat taattttatc ggtgtagata tggttaccgg gctcatcatc atcataccta
     1621 acgttaacaa aacgctggat a
//
LOCUS       DAPGRN010000271         1625 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPGRN010000271 DAPGRN010000000
VERSION     DAPGRN010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.277"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..510)
                     /gene="trbC"
                     /locus_tag="RHW33_004803"
     CDS             complement(<1..510)
                     /gene="trbC"
                     /locus_tag="RHW33_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HDV1648208.1"
                     /translation="MNWKTFALFALTAGMHITVHASDNVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFC"
     gene            complement(538..990)
                     /locus_tag="RHW33_004804"
     CDS             complement(538..990)
                     /locus_tag="RHW33_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648209.1"
                     /translation="MSNNIKYGSRILDMGVLLEMKALVNANGGVSRCIMECRQLACEV
                     RHSQQLLDKLSCIDDWLCRFSSIMYHRFDKEQIQWREDVDRDYPREKVYIFSPDNVSV
                     HNNDIKDSANNTSGITVSITASKICAGQKEFDAFLKLAEKLRENPTGN"
     gene            complement(1020..1481)
                     /locus_tag="RHW33_004805"
     CDS             complement(1020..1481)
                     /locus_tag="RHW33_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV1648210.1"
                     /translation="MYKQATELMLNFKDRILIKGEEDTGKSTLLTEIRISDSDSRYYN
                     FKTLNSAGYNRLCDENIDNFDFLNTPEKTLILDGVRLCEKKMTSKVIRLIKQARKYHK
                     RLVVVADSCESEFIELLFDGVIALSFNSDRERSCNVYTPSRHRNTDNIYAR"
     gene            complement(1507..>1625)
                     /locus_tag="RHW33_004806"
     CDS             complement(1507..>1625)
                     /locus_tag="RHW33_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HDV1648211.1"
                     /translation="QCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
BASE COUNT          417 a          399 c          325 g          484 t
ORIGIN      
        1 gcagaacacc accagtgccg gtacactgcg aatgccgtac tgtgaaaaca gcgtcgggtc
       61 gatctgaaca ccatcagtcg cgccgtcttt caccagggac agcacggctt cagcggtggt
      121 cttcaggtca ttgttcacca tgccccgcag tgtggcgggt ataccgtagt gccgggtttc
      181 gcccagcatc cgtttcagcc cctcttcggg aatggaaaac gacacaaaat acagggcccc
      241 ctgccggggt ttcccgtcct gcgaagcctg ctgtttacga accagctcat ccaggaaatg
      301 gttatctgaa cgctgaaggg ggttctccag cacctgtttc tccgcccagg ccttcagctg
      361 atggtcaggt ttttcacgca gttgtctgct taaattttcc tgctgcttca ggaactggcg
      421 gttttcagga gtgttcacat tgtctgaggc atgaacagtg atatgcattc ctgcagtcag
      481 ggcaaaaagc gcaaatgttt tccagttcat gatatatcct gatggcattg ttacaattta
      541 attacccgta gggttttctc tcaatttttc tgccagtttt aaaaaagcat caaactcttt
      601 ctgacctgcg catattttac tggctgtaat cgacacggtt attcctgatg tgttattggc
      661 agagtcttta atatcattat tatggacgga gacattatcg ggactgaaaa tataaacttt
      721 ttctctggga taatccctgt ccacatcctc ccgccactga atctgttctt tatcaaaacg
      781 gtgatacatt attgaggaaa accggcacaa ccagtcgtca atacatgaca gcttatccag
      841 taattgttgc gaatgacgga cctcacaggc aagctgccga cactccataa tgcaccgtga
      901 cacaccacca tttgcattca caagcgcttt catctccagc agaacaccca tatcaagtat
      961 tctggagcca tattttatat tattactcat tacaccctcc tctgtttttg tttacacatt
     1021 tacctggcat aaatattatc tgtattacga tgccttgatg gcgtataaac attgcaactc
     1081 ctttctctgt cagaattgaa ggacaatgca ataacaccat caaacaacag ttcgataaac
     1141 tcagactcac atgagtctgc aaccacaacc aggcgtttat gatattttct ggcctgttta
     1201 attaacctga taacttttga tgtcattttc ttttcacaca gcctgactcc atcaaggatt
     1261 aacgtctttt caggtgtatt cagaaaatca aaattatcaa tattttcatc acaaagccgg
     1321 ttgtaccccg cagagttaag agttttaaag ttgtaatacc gggaatcact atcactgatt
     1381 cttatttcag tcaggagagt tgacttgccg gtatcttctt cccccttaat cagaatgcgg
     1441 tctttaaagt tcagcattaa ttctgttgcc tgtttataca taaaaaatcc ttcagaatta
     1501 gtagttttac aggaagacac agttacgttt acgccacatc aggtagccga agtttttctt
     1561 tgtattgggc ggatttttcc cggtttccca gcgggtcaca ctgcgcccga acgggtggca
     1621 ctgtc
//
LOCUS       DAPGRN010000272         1586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPGRN010000272 DAPGRN010000000
VERSION     DAPGRN010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.278"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            122..>1586
                     /locus_tag="RHW33_004807"
     CDS             122..>1586
                     /locus_tag="RHW33_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscV/YscV/HrcV family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1648212.1"
                     /translation="MFNKVLVGLRSHPELIILGLMVMIIAMLIIPLPTYLIDFLIGLN
                     LTLAILVFLGSFYVDRILSFSSFPSILLITTLFRLALAISTSRLILLEADAGEIITSF
                     GEFVIGDSLVVGFVIFSIVTIVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLR
                     AGIIDADLAKERRSVLERESQLYGSFDGAMKFIKGDAIANIIIIFVNIIGGLSVGVGQ
                     NGMDFSTALTVYTILTVGDGLVSQIPALLIAISAGFIVTRVNGDSDNMGQNIMSQLLS
                     NSFVIIVTCVLALSIGLLPGFPLLVFLCLAVILGIYFYFKFKKKGTEETVVEGDITVG
                     LEPYNQDDDISLGIINKLDQVITETVPLVLIMNSVQAKKYTEINLADRIRSQFFIEYG
                     IRIPGIVIREGEGLNDEDVILMLNEVRASQFKIYHDLVLLVEYSDEVVSTLIKKPVIV
                     NSNGEQYYWVTKSDAQKLTKIGCYTRTAMDEMYNHLSV"
BASE COUNT          458 a          240 c          342 g          546 t
ORIGIN      
        1 ttattagaga ttagggagtg ttgtaaacag gcaataaaag taacatttgc agcagagctc
       61 tttgatacac aaaactgtaa caaaaaacaa aataaaaaac catggaagaa ggtgatgttc
      121 aatgtttaac aaagttttgg tagggttaag gagtcatcca gaattaataa ttcttggatt
      181 aatggtgatg attatcgcca tgctcataat tccattgccc acctatttaa tcgacttttt
      241 gattgggctt aacttaacat tggcaatact tgtattttta ggatcattct atgttgatag
      301 gatcttaagc ttttcttcat ttccatcaat cctccttatc acaacgttat ttcgtttggc
      361 gctggcgatc agtaccagtc gacttatact tcttgaggcg gatgctggag aaattataac
      421 gagttttggt gagttcgtta ttggagatag cctggtagtt ggttttgtta tcttctccat
      481 tgttactatt gttcagttta tcgtgattac taaaggttcg gaacgtgtgg ctgaagttgc
      541 tgcccgcttt tcgcttgacg gtatgccagg taaacaaatg agcattgatg ccgatctacg
      601 cgctgggatc attgatgcag atttagcaaa agaacgtcgt agcgtattag aacgagagag
      661 tcagctgtat ggttcctttg atggtgcgat gaaatttatc aaaggtgatg ctattgcaaa
      721 cattattatt atctttgtta atattatcgg tgggctatca gtaggagtag gccaaaatgg
      781 aatggatttc tcaacagcgt tgactgtcta cacaattctt actgtcggtg atggtcttgt
      841 ttctcaaatt ccagctttat taattgctat tagcgcagga tttatcgtta cgcgcgtaaa
      901 tggcgatagt gataatatgg ggcaaaatat aatgtcccag ttattaagta attcttttgt
      961 cattattgtg acatgtgtcc tcgcgctaag tattgggtta cttccaggtt ttccccttct
     1021 tgtgttttta tgtttggcag tgatcttagg aatttacttt tactttaaat tcaaaaagaa
     1081 agggactgaa gaaacggttg ttgagggaga tattaccgtt gggttggaac cctataatca
     1141 agatgatgat atctcattag gtatcattaa taaacttgat caggttatta ccgaaacggt
     1201 acccttggtc ttgattatga atagtgtaca ggctaaaaaa tacactgaaa tcaatcttgc
     1261 tgataggatt cgtagtcagt tttttattga atatggtatc cgaattcccg gtattgtcat
     1321 tcgtgagggg gaaggtctta atgatgaaga tgtcatatta atgctaaatg aagtcagagc
     1381 ttcgcaattt aaaatttatc atgatcttgt cttgttggtt gaatattcag atgaagtggt
     1441 ttcgactttg atcaagaaac ctgtcattgt aaatagtaat ggtgaacaat attattgggt
     1501 tacgaaaagt gatgctcaaa aattaacaaa aattggctgt tatactcgca cggccatgga
     1561 tgaaatgtat aatcatttat ctgtat
//
LOCUS       DAPGRN010000273         1563 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPGRN010000273 DAPGRN010000000
VERSION     DAPGRN010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.279"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(108..512)
                     /locus_tag="RHW33_004808"
     CDS             complement(108..512)
                     /locus_tag="RHW33_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HDV1648213.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLADAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(733..1464)
                     /gene="bluR"
                     /locus_tag="RHW33_004809"
     CDS             complement(733..1464)
                     /gene="bluR"
                     /locus_tag="RHW33_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV1648214.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          426 a          345 c          326 g          466 t
ORIGIN      
        1 ataaaagaac agtatttatc atccataggg atagtctctg tacttttatt cccattatgc
       61 taatgcctta ctgaattatg aagcatttct taagcatcca actttagcta gattaatggt
      121 ttattatttt ctacatcttc aatatataaa agcgtattat caatggcgta gtaactgcgt
      181 ttgttatgat taacatcagt aacccaacgg aaaacgcccg cgtctgccag tgttgaacag
      241 tattcccgaa atgtagattt tccgcaaata tgaagcaatg cggcctcttt tattttagca
      301 gggttcttgg tcgtactaac ttttaacagg ttcctggttc ctcttaataa caaaaccgtg
      361 tcatcgtgag taataattct gatgttatcc gtagccagat aataaatgta atgtgcaata
      421 cggtgatgtt ttaattctga ataaaaccag gagaagtttt gctcttttct cacttgctca
      481 aacatctttt gaaaaacaac gacctgatcc atcaggataa taacctcttg ttagttgtga
      541 gactgcgtag tgtgcacgat cggttttacc acttcaatct ggtctgtcct ttggctgtga
      601 tatgtacaaa gtgtgaaagg gggattacct gaattctccc ggtgagcatt ttgcaacgga
      661 ccagctccgg tacaaatgct gttgtgggtt cagattataa cattctgtct aaggggcggg
      721 ataaaggtga aattaggggg catgaaagat gactttataa ccttgctcac cccagtgttg
      781 taaaagttcg ttttgccttc tcgttggtgc catgcctgtc cagacaatca atgtttgcgt
      841 cgggaacagt tccgggcgcg gtgattcaat gggatcagca agaacagaaa tatgccatcc
      901 tgacaatgat aaacgccatg cttccagcca caatcgagct ctctcttcaa cattccacgc
      961 catcagaatg gcttctttac cgggcttacg gcgcatttcg aaaagcgagg ctgctgcata
     1021 ttcaattaat gcgccgtcaa acatactgct cataatgcgg gaggtgttgt gatcaagcac
     1081 gagacgctgg cgaacaggaa ggtaaacatg attaatcaat tgatctactg ggtactctcg
     1141 acccagtgaa ataattctcg cgcgtagttt ggcaggatta gccatacgaa gaattgacat
     1201 catctcttct tgcaggcggc tccagtcatc ttccgtatcc tggctggtgg tttccagtaa
     1261 tgctttaact ttgcctacag ggacgccatt acttatccaa cgcttgatct cttcgatacg
     1321 ttgtatgtct tcttcatcaa agagtcggtg tccgccttca ctgcgctgtg gttttaacaa
     1381 accgtagcgg cgttgccagg cccggagagt gacaggatta atcccgcaac gttcagcaac
     1441 atcaccaatg ctgtaataag ccacaattcc tccttgcggt cacaaatctc cgtcgcctgt
     1501 acacgaccca ataatacttt gtacaatata cgctaaaatt gtacaaagta taaataagat
     1561 ttc
//
LOCUS       DAPGRN010000274         1552 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPGRN010000274 DAPGRN010000000
VERSION     DAPGRN010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.280"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(924..1400)
                     /locus_tag="RHW33_004810"
     CDS             complement(924..1400)
                     /locus_tag="RHW33_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV1648215.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEELEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          345 a          417 c          390 g          400 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg ccctttgggg gtgtcggggc gcagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctcctgcacg ttttttgccg tccgttcacc gttaaactcc
      241 gcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgtatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggatga
      481 gtcgtcagat agcccttcaa actcgctcag aagctctcag acgcacgaga cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagccactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcggt cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aatcatggtc agaacgttaa cacgcagccc
      901 ctgattgtcc gcctgccgct ggctcaattt gaaaacatgg ccatgattcg gcgctgaata
      961 tcaccgtctt tgttgaccag tgttttagtg acaaacgctg catggttttt cgtagtgtta
     1021 tatcccttcc agacttcatg tggatcgagg gcatacgcac aaaccttacc agcaacgggg
     1081 attctaacta atgcattttt tgattctaag taattgagcc accgaataat tgtgcttctg
     1141 ctaactccaa gtttttcagc taggaattgc tgatccgcaa cgacagcgcc acacgtagga
     1201 tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg atatgccttg gctatccttc
     1261 agcagctctc gaacgcgctc ccaacctttt ggggatacct gtgtaaacct tgaattcttc
     1321 tttgcttctt cgagctcctc ggtgtttttc tttgcctcat ctgaaagttg tttcagtcgt
     1381 ttttctgaat caataaacat gattttttcc ctctgaaaat cccatgcact aacgttggtt
     1441 caaatctaag ccatcatgac acatctgtgc aacaccttgt tgcgtgttgt cgcatccagg
     1501 atgcgacaac gttgtgtcac cctagatgcg acaggtaaac aaaaaacaca ct
//
LOCUS       DAPGRN010000275         1494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPGRN010000275 DAPGRN010000000
VERSION     DAPGRN010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.281"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..564
                     /locus_tag="RHW33_004811"
     CDS             <1..564
                     /locus_tag="RHW33_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV1648216.1"
                     /translation="TIAGDKTDEWLQPACNEMYRVLKKDALMVSFYGWNRIDRFMAAW
                     KRAGFSVVGHLVFTKNYTSKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNR
                     HHPTEKPVTSLQPLIESFTHPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRA
                     GQQRLAAVQRAMQQGAANDDWFEPEAA"
     gene            565..786
                     /locus_tag="RHW33_004812"
     CDS             565..786
                     /locus_tag="RHW33_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648217.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            800..1234
                     /locus_tag="RHW33_004813"
     CDS             800..1234
                     /locus_tag="RHW33_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV1648218.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            1285..>1494
                     /locus_tag="RHW33_004814"
     CDS             1285..>1494
                     /locus_tag="RHW33_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648219.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLAS"
BASE COUNT          349 a          423 c          439 g          283 t
ORIGIN      
        1 accattgctg gcgataaaac cgacgaatgg ctgcaaccgg cctgtaatga gatgtaccgc
       61 gtgctgaaaa aagacgcgct gatggtgagc ttctatggct ggaaccgtat tgaccgcttt
      121 atggcagcct ggaaacgcgc cggattcagc gttgtagggc atctcgtctt caccaaaaac
      181 tacacatcga aggccgcata tgtgggctat cgccacgaat gcgcctatat cctggcaaaa
      241 ggccgtccac gtctgccaca aaacccgctg ccggacgtgc tgggctggaa atattcaggc
      301 aaccgccatc acccgacgga aaagcctgtt accagcctgc aaccactgat tgagagcttc
      361 acacatccga acgcgattgt gctggacccg tttgcaggca gcggctcaac ctgcgtcgcc
      421 gcccttcagt ccggacgccg gtatatcggt atcgagctgc ttgaacagta tcaccgtgcc
      481 ggacagcaac gactggccgc cgtgcaacgg gccatgcagc agggggccgc gaatgatgac
      541 tggtttgaac cggaggctgc gtaaatgaac tatgccggac acgaaaaact gcgcgccgag
      601 gtggcggagg tggccaatgc catgtgcgac ctgcgtacaa ccatgaatga gatggagcag
      661 cggtacagct ttaatgccga caccctgccg gaacgtctgg tgcgtcagac gctgtttcgc
      721 gcaaaccgcc tcctgatgga ggcatatacc gaaattctgg aactggaagc gtgctttaaa
      781 gattgagaag gagacgagaa tgtacggaac atgcgaaacg ctatgccggg agctggcagt
      841 aaagtatccg ggagacatgc cgctgatgct ggttatctgg tccccggaag agattcaggc
      901 actggctgac ggtatggaaa tttctctgac cggtcatgaa atcaggaccg tcctggcacg
      961 cctggaggac atcccggaag accagcggat tgagtccggt atttcttccg ctgcggtgat
     1021 ggagattatc cgcaacgaga gcgaaaaccg cctggtgacc gtccctgctg aactgctggc
     1081 gtccctgatt cagaccgctg aacaggcatt gtggaaacgt gaatgggccg cccgggatca
     1141 tggcctcgcc gtcccggaat gcgtcacccg ccgtcaggag gtggttaatc aggcccgcac
     1201 cctgctgaaa aacaacacac acgaaaacga ctgatgttat cgccgcctgc cctgcgggcg
     1261 gcgatacagg gagaacgctt aatcatgaac gaaaacagca cgctgaacgc actgatttgt
     1321 cgtcacgccc gcaacctgct gctggcgcag ggctggccgg aagagacgga tgttgaccag
     1381 cgtaacccga agtatccggg ctggatcagc atttatgttc tgctggatgc gccccggctg
     1441 gcgacgttac ttatcaaccg tcacggcggc gtactgccgc cgctcctggc ctcc
//
LOCUS       DAPGRN010000276         1487 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPGRN010000276 DAPGRN010000000
VERSION     DAPGRN010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.282"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1487
                     /gene="espL1"
                     /locus_tag="RHW33_004815"
     CDS             <1..>1487
                     /gene="espL1"
                     /locus_tag="RHW33_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HDV1648220.1"
                     /translation="SLLLETTPIKANHFRSILDYIEQEQLTAENRNHCMKLSKKIHRE
                     KTIQPTVNLNGSAFFSQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQ
                     HVPLVRDAEFRALLRHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSTILYIDNHT
                     LSVRLRIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWA
                     DRYVRNCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLITLFR
                     QLPILPEKEIIEIITAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLVDNHIIHEDNL
                     VKLMQTKSANETPGLYISMLYGFDEIIDIFLNTLTAPIAQKHLNKKMVMDILAMKTRD
                     GEPGLFAAMENNHPLCVTRFLSKIYGIAVKYNLSKINIIDLLKGATVHGTPALYIAMS
                     KGNKDVMLSYISTLDTFAKKYSFSQHQLFTLLAAKNHDNMSAVHI"
BASE COUNT          463 a          312 c          279 g          433 t
ORIGIN      
        1 tttccttatt actggaaacc acgcctatta aagctaacca ttttcgaagc atactggatt
       61 atattgaaca ggaacagtta actgccgaaa atcgtaacca ttgtatgaaa ctgtctaaaa
      121 aaatccatag agaaaaaact atacaaccaa cagtaaatct caatggtagt gcattttttt
      181 cgcaatctcc ttctgacgct attttttgtc gccatctgtc attgcaatac gcacttgatt
      241 cattgagaaa tggaaaaggc aaggtcaacc tgattaaaca ttactcctcc gttgaatcca
      301 tacagcagca tgtcccctta gtccgggacg cggagttcag agcattactt cgccatcctc
      361 ctgcagggag tcgcgttatc gcgagtaagg attttggctt cgctttagat attttcttct
      421 gtcgaatgat ggcaaacaat gtcagtcata tgtctacgat tttatatata gacaatcata
      481 ctttgtcagt aaggctacga ataaagcagt cggcgtatgg gcaattaaat tatgttgtgt
      541 ccgtttacga cccgaacgat accaacgttg ccgtcagagg cacccacagg acagcacggg
      601 gctttctctc gcttgataag ttcatcagtt caggtcccga tgctcagacc tgggctgata
      661 ggtatgttcg caactgtgca attgctattc tgcccctatt acctgaggga gttccagggg
      721 ctattttcac gggtatcgcg acacgaatgc catttgcccc tatacatcca tcggcaatgt
      781 tgttaataat ggccacaggc cagactcaac agcttattac attatttaga cagttgccca
      841 tactccctga aaaagaaatc attgaaataa taactgcgca gaatagcgtt ggtacacctg
      901 ctttattttt ggctatgatg aacggacata ctgacaacgt gaaaatattt atgcaagaaa
      961 ttcagtcact ggtagataat cacatcattc atgaagataa tctggttaaa ttaatgcaaa
     1021 ctaaaagtgc taacgaaaca cctggacttt atatctccat gttgtatgga ttcgatgaga
     1081 taatcgatat ctttctgaat acattaaccg ctccaatagc acaaaagcat ttaaacaaaa
     1141 aaatggtgat ggatatttta gccatgaaaa cacgtgatgg tgaaccaggg ttatttgctg
     1201 caatggaaaa taatcacccg ttgtgtgtca cacggttcct ctctaaaatt tatggaatcg
     1261 ctgttaaata taacctcagc aaaattaaca tcatagattt attaaaaggc gcaacagtac
     1321 atggaacccc tgctttatac atcgctatga gcaagggtaa taaagacgtc atgttatctt
     1381 atatatcgac gctggatact tttgcaaaaa aatattcttt tagccaacat cagttattta
     1441 cactgttggc cgctaaaaat catgacaaca tgtcagctgt tcatata
//
LOCUS       DAPGRN010000277         1453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPGRN010000277 DAPGRN010000000
VERSION     DAPGRN010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.283"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            541..707
                     /locus_tag="RHW33_004816"
                     /pseudo
     CDS             541..707
                     /locus_tag="RHW33_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112096.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(742..882)
                     /gene="ykgO"
                     /locus_tag="RHW33_004817"
     CDS             complement(742..882)
                     /gene="ykgO"
                     /locus_tag="RHW33_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV1648221.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(882..1145)
                     /locus_tag="RHW33_004818"
     CDS             complement(882..1145)
                     /locus_tag="RHW33_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV1648222.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTAREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
BASE COUNT          425 a          287 c          327 g          414 t
ORIGIN      
        1 tttatattgg tcaggaactt tgccctgaag tgttaggaat agtcttaatg cgctgtgtga
       61 tggactagta aaaaaacaag ttttaacggt aagggataga atataaaact ataatgagct
      121 gtcattgaag atgatttgcc aatcagtaag aaattttaat ggattaacat gccgcacaaa
      181 atggttatag gtgttcatta caataagtga tggaaaattg aacgggcgta tgtacgtcat
      241 ctgttcggta agcccccaag agccagatta agtgaagtta aacgaaggct gccagctgtc
      301 acaggaacga tggcctcttt gtcagggtac tgagtgcagt tatctgtgcg cgttattcta
      361 actggcactc gaacgtcggc gacggggtta ccggtggatc agactgttcc tgtggcgtaa
      421 acgtttttat gttagtacca acagatttac cacatttatc atcttaacag tttgagtgaa
      481 aatgcaggct gcgccgcaaa gggaggagac tgagtgtgtg ttcagtctaa aagagagttg
      541 atgccagcat acaggggcga agctggtcaa caagtcaaca ataagataat ggaatattcg
      601 gagagaaacg tcaggcagtt ggcctcaaat gaacaggaag atacatcccc aggaaaatta
      661 atgctggtgt aataaataca ccaacattaa accgatctga ttattaatca gacaaatatc
      721 gttaatccgt cgaattttga atcaacgttt tttcttacga ccctgaacgg ccttaaaacg
      781 tggattagat ttacaaatca catatagccg tccttttcgc ttcacaatct gacagtctgg
      841 atggcgttct tttgcggtac gaagagagtt aagaactttc atcacgcccc ctttttcgtg
      901 ctaacaaaac gaccaaaacg ttgggtgaat cgtgcaacat ttccttctga tgccactgtt
      961 ctcagcttcc ctgtatagaa cgggtgcgat ttagaagaga catcaattgt cacgtacgga
     1021 tacgttacgc catccagctc aatctcacgg gctgttttga tagtcgagcc gattttaaag
     1081 tactcatcaa cactggtgtc gtggaacact acagtacgat actcaggatg gatattgggc
     1141 ttcatcattt ttacctgtta tgttataaca taactataaa gatacatgct catccggatc
     1201 atttcaacac ccaatccctg tgaccctttg tggatgtgga atttcctcag cggggaagtc
     1261 tggcattgtt aacagccatt ggcgacgctc ccataaacgc ctggatgagt gattgatgat
     1321 agtttcgctg agagtatgga tttttcgaga atggaggtta tctccacttt actggccgat
     1381 gatttatcgc agtatgattg attacctgac agcgtgtatt cctctcccac ggatataata
     1441 attacttaca aaa
//
LOCUS       DAPGRN010000278         1448 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPGRN010000278 DAPGRN010000000
VERSION     DAPGRN010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.284"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            181..1392
                     /gene="bluF"
                     /locus_tag="RHW33_004819"
     CDS             181..1392
                     /gene="bluF"
                     /locus_tag="RHW33_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV1648223.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANDLVPEQIIVEF
                     TESEVISRFDEFAETVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSVAWPEKK"
BASE COUNT          414 a          306 c          305 g          423 t
ORIGIN      
        1 taaaacatat tgcatcattt aactgacaac ctatacaaaa attttcgcca actcgtgttc
       61 aactttttcc attttttatc aatgtaaaga aacagttatg ttttggtatt aaaagaaaga
      121 caccggaaga tatgtctgtt accgtcttac tctcgcctct cccattaccc tggacagtta
      181 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
      241 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      301 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      361 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      421 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      481 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      541 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      601 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      661 cttgattcct gtaccctttc accaactata aacgaccact ttgcctttca tcctattgtc
      721 gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagacagc
      781 ccgtcagcta tagcggttgg tcagcgtaaa gacggggaaa tctacacagc ggatcttaaa
      841 agtaaggcgc ttgcattcgc gatggcacac gcacttgagc tcggtgataa aatgatatca
      901 atcaatctat tacctatgac cctggttaac gagcctgacg cagtctcttt tttacttgat
      961 gaaataaagg ccaatgatct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa
     1021 gtcatatctc ggtttgatga gtttgccgaa acggttaaat cgctaaaggc tgccggtatc
     1081 agtgtagcaa ttgatcattt tggcgcaggt tttgctggtt tgttactcct gtcacgcttc
     1141 cagcctgaca gaattaaaat cagtcaggaa ttgattacca atgttcataa aagcggacca
     1201 cggcaggcaa ttattcaggc gatcatgaaa tgctgtacat cacttgaaat tcaagtctct
     1261 gctatgggcg tggcaacacc agaagagtgg atgtggcttg aatctgcagg aattgagatg
     1321 tttcagggag atctgtttgc caaagctaaa ttgaatggta tcccttcagt tgcgtggccg
     1381 gagaaaaaat aattttcagc acattatttc gcatgttttc tggaaatcat cgtgaaaatg
     1441 aatacgtt
//
LOCUS       DAPGRN010000279         1441 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPGRN010000279 DAPGRN010000000
VERSION     DAPGRN010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.285"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            119..1099
                     /locus_tag="RHW33_004820"
     CDS             119..1099
                     /locus_tag="RHW33_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HDV1648224.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            1092..>1441
                     /locus_tag="RHW33_004821"
     CDS             1092..>1441
                     /locus_tag="RHW33_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDV1648225.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQ"
BASE COUNT          400 a          262 c          350 g          429 t
ORIGIN      
        1 gtttttatgt tgcatttgtc ctttcaaaaa gaccgttttt tcattataat tgaatataca
       61 ttgcatgtat gaagtatgtt tgttttttct atttgtttat gagcaacgga gactggttat
      121 gaaaatcttt attgatgatg gttctaccaa cattaaactt gcctggttgg aggatggtga
      181 cgtgaaaacg ttaatcagcc ctaacagctt taaaccggaa tggtctttta gtttactgga
      241 tgatgctgct ccggctaatt acgagatcga cggcgagaag ttttcatttg atccattaag
      301 tgctgatgct gtcgtaacga ctgaaacacg ctatcagtac agtgatgtga atgttgtggc
      361 cattcagcat gcgctacagc agaccggcct gaaagcgcag cctgttgatg tcatcgtcac
      421 attgccgatc tctgaatacc ttgacgccaa caatcagaaa aataagcaaa acattgaaag
      481 aaagaaaaaa aatgtgatgc gtgaagtacg ggtgcagggg agtgatgcat ttgttattcg
      541 cagtgtttcc gttcttcctg aaagtattcc cgctggtttc agtgttcttg cggggcttga
      601 ggatgatgaa tcccttttga tcgtagatct cggtggtaca actcttgatg tgtcgcatgt
      661 tcgtagcaaa atgacaggga ttacaaaaac ctggtgtgat ccgaatatcg gcgtgtccct
      721 gattacatcc ggggttaagg aacagatggc tgtacatgct aatacaagag tgagctcatt
      781 ccaggctgac aatatcattg tgcaccgtaa tgaacctgac tacctttccc gtcgcattta
      841 taatgctgaa cagcgtgagt ctatcattaa tgttatcaat gaacggcaga agttgcttat
      901 taaacgagtt aatgatgtca tcagccgttt caccgactat acacatgtaa tgtgtgttgg
      961 tggtggggct gaaattgtgg ccgaggctgt gaagaacctg actaaggttc ctgatgagcg
     1021 cttttatctg agctcatccc cgcagtttga tttagtcatg ggaatgataa aaatgaaagg
     1081 tggtgtgact aatgagtgat gaaaataaat ccagacgctg ctcatttgaa cttttcccgg
     1141 atgaacggac aggagataag atagcagatg agctcattgc aaatgaaaaa ctgaaggagc
     1201 ggggacgttt catgcgcgcg atgttagtta ctggtgcggc gtttgcagct atagataaac
     1261 gtcttccttt gctgatatcg gagttactga cagaaaatac gactctggat gatattaata
     1321 aggtgatttc cagcgtaata ccaggtgctt tttcggttga aaagaaattg cttgaattac
     1381 tggaaaaaca atctggtctc catacgtctg tggattgcag tactcctctt acagagcaga
     1441 g
//
LOCUS       DAPGRN010000280         1411 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPGRN010000280 DAPGRN010000000
VERSION     DAPGRN010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.286"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1334)
                     /locus_tag="RHW33_004822"
     CDS             complement(<1..1334)
                     /locus_tag="RHW33_004822"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV1648226.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKEGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASL"
BASE COUNT          332 a          380 c          377 g          322 t
ORIGIN      
        1 agggaggctt tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga
       61 cacataatgc gcatcaccac gatgtcggtg gcttcaccgc cgagagtgaa ggccggaacc
      121 tgccagccgc gcagacgcag acgttcagag aggtcgtaca gggtgtatcc cggatcttca
      181 ccctctttca gtttgaagca aaccgccggg atgccttcgt ccgggcgacc cgtacagatg
      241 aactcatacg gccccagttt ggcgatttca tccgccagat aagcggcaac ctggtaagag
      301 gcgttctgta ctttggtata gccttcacga ccgaggcgca ggaattcata gtactgtgca
      361 attacctgac ctgccgggcg ggagaagttg atggcaaaag taccaatctg accacccagg
      421 tagtcaacgt tgaacaccag ttcctgcggc agcgcttctt cgtcacgcca gataacccag
      481 ccgcagccca gcggagccag accgaattta tggcctgaag cactgatcga tttcacacgc
      541 ggcaggcgga agtcccagac gatatccggg gcgacgaacg gtgccaggaa gccaccgctg
      601 gcggcgtcga tgtgcatgtc gatgtcgata ccggtgtcgg cctggaattt atccagcgca
      661 tcgtgcagcg gttgcgggaa ctcatagtta ccggtgtagg tcacgccgaa agtcggcacc
      721 acgccgatgg tgttttcgtc gcaggcttca atcatgcgtt tcgggtccat aaacaactga
      781 ccggggcgca tagggatctc acgcagctcc acatcccagt agcgggcgaa tttatgccag
      841 cagatttgta ccggaccgca caccaggttt ggtttatccg ttggtttgcc tgcagcttcc
      901 atacgcttgc gccaacgcca tttcatcgcc atcccgccga gcatacaggc ctcggaagaa
      961 ccaatggtgt tggtgccaac ggcctgacca tttttcggcg caggcgcatg ccacagatcg
     1021 gcaaccatgt ttacgcaacg caggtcgatg gctgcggatt gcggatattc ttctttgtcg
     1081 atccagtttt tattgatcga caaatccatc aatttatgga cgttttcgtc gtcccaggtc
     1141 tggcagaaag tggccaggtt ctgacgagcg ttgccatcaa gatataattc atcattgata
     1201 atctggaatg cgacatcatc gcgcatttcg tgcagcggaa atcgttttga ctccgcgata
     1261 gtagaaatgg cctttgcgcc aaaacgtgaa tcgagtagtt ctgagcggaa atccgttaac
     1321 agcttctggt ccatttcgaa ctccttaaat ttatttgaag gcaataaaaa agtaggattt
     1381 atccgcaatg gaagcaaggc attacaggct t
//
LOCUS       DAPGRN010000281         1392 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPGRN010000281 DAPGRN010000000
VERSION     DAPGRN010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.287"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            146..367
                     /locus_tag="RHW33_004823"
     CDS             146..367
                     /locus_tag="RHW33_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HDV1648227.1"
                     /translation="MGYSAAKVSTHIELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            375..>1392
                     /locus_tag="RHW33_004824"
     CDS             375..>1392
                     /locus_tag="RHW33_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV1648228.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMGNSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLL"
BASE COUNT          361 a          308 c          376 g          347 t
ORIGIN      
        1 agttcaatac cttatcaggt caaatagtga tcacttgatc atttgatcaa ggttgcgcta
       61 cgtaaaatct gcgaaatgtt ggcagtgtta gtgctccaga tttcgcgtag cgcacttagc
      121 accaccaatc aatcagaggt gaaaaatggg atattcagct gctaaagtgt ccactcatat
      181 tgagcttgag aaaaaccgtg gttactggcg ggcaaaaggg tttgatcgtg atagttgtca
      241 actgtcatta tcgcgcggtg aagagaaaat agaacgcacg cgcggtcgct ggcgtttcta
      301 tgacgagaac cataaacagg taaaggcaga gccgatcctg tacactttac ttaaaaccat
      361 tatctgagtg ttaaatgtcc aatttactga ccgtacacca aaatttgcct gcattaccgg
      421 tcgatgcaac gagtgatgag gttcgcaaga acctgatgga catgttcagg gatcgccagg
      481 cgttttctga gcatacctgg aaaatgcttc tgtccgtttg ccggtcatgg gcggcatggt
      541 gcaagttgaa taaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc
      601 tatatcttca ggcgcgcggt ctggcagtga aaactatcca gcaacatttg ggccagctaa
      661 atatgcttca tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg
      721 tcatgcgacg gatccgaaaa gaaaacgttg atgccggtga gcgtgcaaaa caggcgctgg
      781 cgttcgaacg cactgatttc gaccaggttc gttcactcat gggaaatagc gatcgctgcc
      841 aggatatacg gaatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg
      901 aaattgccag gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc
      961 atattggcag aacgaaaacg ctggttagca ccgctggtgt agagaaggca cttagtctgg
     1021 gggtaactaa actggttgag cgatggattt ctgtctctgg tgtggctgat gatccgaata
     1081 actacttgtt ttgccgtgtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc
     1141 tatcaactcg cgccctggaa gggatttttg aagcaactca ccgattgatt tacggggcta
     1201 aggatgactc tggccagagg tacctggcct ggtctggaca cagtgcccgt gtcggagccg
     1261 cgcgagatat ggcccgcgcc ggagtttcaa taccggagat catgcaagct ggtggctgga
     1321 ccaacgtaaa tattgtcatg aactatatcc gtaatctgga tagtgaaacg ggggcaatgg
     1381 tgcgcctgct gg
//
LOCUS       DAPGRN010000282         1380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPGRN010000282 DAPGRN010000000
VERSION     DAPGRN010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.288"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..559
                     /locus_tag="RHW33_004825"
     CDS             <1..559
                     /locus_tag="RHW33_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDV1648229.1"
                     /translation="FAEAVSQLAALAAAGKDVAALNGVDVRAFTGQAKFCKKAAAGYS
                     NCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGVCLEKKRSYCQF
                     DSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQ
                     KIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            586..843
                     /gene="trbE"
                     /locus_tag="RHW33_004826"
     CDS             586..843
                     /gene="trbE"
                     /locus_tag="RHW33_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDV1648230.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTAGEIL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            836..>1380
                     /gene="traF"
                     /locus_tag="RHW33_004827"
     CDS             836..>1380
                     /gene="traF"
                     /locus_tag="RHW33_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDV1648231.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQVQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDG"
BASE COUNT          370 a          306 c          383 g          321 t
ORIGIN      
        1 ttttgccgaa gctgtttctc agctggcggc actggccgca gcaggtaagg atgttgcggc
       61 gctgaacggg gtggatgtcc gtgctttcac cggtcaggcg aaattttgta agaaggctgc
      121 agccggatac agcaactgct gtaaggacag cggctgggga caggatatcg ggctggccaa
      181 atgcagcagt gatgaaaaag ccctggctaa agcaaaatca aacaaactga ccgtcagtgt
      241 cggagagttc tgttcgaaga aagtgctggg tgtctgtctg gagaaaaaac gcagctactg
      301 tcagtttgat tcgaagctgg cgcagatagt ccagcagcag gggcgcaacg gacagctgcg
      361 tatcagtttt ggcagtgcga agcatcctga ctgtcggggg attacggttg atgagctgca
      421 gaaaattcag ttcgacagac tggatttcac taacttctac gaagacctga tgaataacca
      481 gaaaatcccg gacagtggtg ttctgacgca gaaagtgaaa gagcagattg ctgaccagct
      541 gaaacaggca ggacagtaag gtagggaaaa taaccgggga aaatgatgaa agtcatattc
      601 acgtcgaacc gttttattga ttttcttatc cggcttttga ttacagcgat tgtcatttcc
      661 ccggtaatta tctggtcgtg ggatacggta aaagaaacca ctgcaggtga gatacttgct
      721 gctgctttcg ttatttttta ctccggtgtg ctcctgttta ttctgtattt ttgtttttct
      781 gccctgactg acctgcagaa acctgatgaa agaaaaagtg atgaaaggaa tgaagatgaa
      841 taaagcatta ctgccactgt tactctgctg ctttattttt ccggcgtcag gaaaagatgc
      901 aggctggcag tggtataacg agaaaataaa tccgaaggaa aaagaaaata aaccagtacc
      961 tgcagccccc cgtcaggagc cggacattat gcagaaactg gccgcactgc agacggcaac
     1021 aaagcgggcg ctgtacgaag ccattctgta tcccggcgtg gataattttg tgaaatattt
     1081 ccggctgcag aattactgga ctcagcaggc cgggcttttc accatgagcg ccaaaaaagc
     1141 catgctggcg catcccgaac tggactataa cctgcagtac agccattaca acggcacggt
     1201 ccggaaccag ctggcggcag accaggtgca gcagcgacag gccattgcga aactggctga
     1261 acactacggc atcatgttct tttaccgggg gcaggacccc atcgacgggc agctggcgca
     1321 ggtcattaat ggcttcaggg atacctatgg tctgagtgtt attcccgttt ccgtggatgg
//
LOCUS       DAPGRN010000283         1351 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPGRN010000283 DAPGRN010000000
VERSION     DAPGRN010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.289"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..1243
                     /gene="ompC"
                     /locus_tag="RHW33_004828"
     CDS             131..1243
                     /gene="ompC"
                     /locus_tag="RHW33_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1648232.1"
                     /translation="MKKITVALSAVAASVLMAMSAQAAEIYNKDGNKLDLYGKVNAEH
                     YFSSSASDDGDKTYARLGFKGETQINDQLTGFGQWEYEFKGNRTESEGSDKDKTRLAF
                     AGLRFADYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTQRATGVATYRNN
                     DFFGLVDGLNFALQYQGKNDSAAKVNNWKGRDVVEYNGDGFGLSATYDYEGFGVGATY
                     AKSDRTDGQVSYAKKDPLNASGKTAEVWATGLKYDANNIYLAATYSETQNMTVFGDDF
                     IANKAKNFEAVAQYQFDFGLRPSIAYLHSRGENIGAFGSQDLVEYIDLGATYYFNKNM
                     SAFVDYKINLIDESEFTKRSEVATDNIVAVGMTYQF"
BASE COUNT          383 a          266 c          310 g          392 t
ORIGIN      
        1 tttgtgattt tgatcacaaa aataccttga tttttatttg caaaacttga aacacgaatc
       61 caaaaaagat aaacatttgt ccgcagtgac ttcttttcta ctgaaaagtt caatctaaag
      121 ggcaaaaaaa atgaaaaaaa tcacagtggc actctctgcg gttgcagcat ctgtcctgat
      181 ggcaatgtct gcgcaagcag ctgaaatcta caacaaagac ggtaataaac tggatctgta
      241 cggcaaagtt aacgcagaac attacttctc ttcttcagct agtgatgacg gagataagac
      301 ttatgctcgt ctgggattca aaggtgaaac tcagattaat gaccagttaa ccggttttgg
      361 tcagtgggag tacgaattta aaggtaaccg tactgaatct gagggctctg ataaagataa
      421 aactcgtctt gcatttgctg gcctccgttt tgctgattat ggttcaatcg actatggtcg
      481 caactatggt gtagcttatg atattggcgc ttggactgac gtgctgcctg agtttggtgg
      541 agatacctgg actcagaccg atgtatttat gacccagcgt gctactggcg tagccaccta
      601 ccgtaacaat gatttcttcg gcctggttga tggtctgaac tttgctttgc agtatcaagg
      661 taaaaatgat agtgctgcaa aagtgaacaa ctggaaaggc cgtgacgtag ttgaatataa
      721 tggtgatggc tttggtctgt ctgctactta cgattatgaa ggatttggcg ttggtgcaac
      781 ctatgcaaaa tctgatcgta ctgatgggca ggtatcctat gctaaaaagg atcctctgaa
      841 tgcttctggt aaaactgctg aagtatgggc tactggcctg aaatatgacg caaataacat
      901 ttacctggct gcaacttatt ctgaaactca gaatatgact gtcttcggtg atgactttat
      961 cgcgaacaag gcgaaaaact ttgaagctgt tgctcaatac cagtttgatt tcggcctgcg
     1021 tccgtccatc gcttatctgc actctcgcgg tgaaaatatt ggtgcgttcg gcagccagga
     1081 tctggttgaa tacatcgact tgggtgcgac ttattacttc aacaaaaata tgtccgcttt
     1141 cgttgactac aagatcaacc tgattgatga aagtgaattt accaaaagat ctgaggttgc
     1201 aaccgacaac atcgttgctg ttggtatgac ctaccaattc taattttggt aggtaagaat
     1261 atgcgggaag ggagtgatgt cactgcccgc atataggtgg caccctcatg ccacctttga
     1321 agaggcaata aaattgcctc ttctcaattt a
//
LOCUS       DAPGRN010000284         1325 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPGRN010000284 DAPGRN010000000
VERSION     DAPGRN010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.290"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..1208
                     /gene="ompC"
                     /locus_tag="RHW33_004829"
     CDS             108..1208
                     /gene="ompC"
                     /locus_tag="RHW33_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV1648233.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDTRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGVATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          379 a          275 c          289 g          382 t
ORIGIN      
        1 gtttttaatc accactctaa tttaaaaaca gtatgtcatt tataaatgac gccgtttaaa
       61 attcgtttag aaaatacgtt taacgataat taaaaggatt ctgttaaatg aaattaaaaa
      121 tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt agcacacgct gccgaagtct
      181 ataacaagga tggtaataaa ctcgaccttt atggcaaggt taccgctcta cgttatttta
      241 ctgatgatac gcgtgacgat ggtgataaaa cttatgcccg tctcggcttt aaaggagaaa
      301 cgcaaatcaa tgatcaaatg attggttttg gtcactggga atatgatttt aaaggctata
      361 acgatgaagc caacggctcg cgcggcaaca agacccgtct ggcctatgct ggtttaaaaa
      421 ttagtgaatt tggctctctg gactatggcc gtaactacgg tgtcggctat gacattggtt
      481 catggactga tatgttgcca gaatttggtg gcgatacctg gagtcagaaa gatgtcttca
      541 tgacataccg taccaccggt gtagcaacct atcgcaacta cgatttcttt ggcttaattg
      601 aagggctgaa ctttgccgcg caatatcaag gcaaaaatga acgtactgac aacagtcatc
      661 tttatggtgc tgactacacg cgtgccaacg gtgacggttt cggtatctcc tcaacttatg
      721 tttatgatgg ctttggtatc ggagcggtgt ataccaaatc cgatcggaca aatgcgcagg
      781 aaagagccgc tgctaatcct ctcaatgcct ccggtaagaa tgcagaactg tgggctacag
      841 gtataaaata tgatgccaac aacatctact ttgcagctaa ttacgctgaa acattaaaca
      901 tgaccaccta tggcgatggt tatatttcta acaaagcaca aagttttgaa gtggtggcgc
      961 aatatcaatt cgacttcggc ttgcgcccct cactcgctta cctgaaatcg aaaggcagag
     1021 atctgggccg ctacggcgac caggacatga ttgagtatat cgacgttggt gcgacgtatt
     1081 tcttcaacaa aaatatgtcg acctatgttg attataaaat caacctgatt gatgaaagcg
     1141 actttacccg tgccgtagat attcgcaccg ataacatcgt cgctacgggc attacctatc
     1201 agttctaacg tcctctcctg accagccctt ctctgggctg gtttttgatt taagtgctgc
     1261 tgtatgactc cttaagtaaa aatgagcaat ttttgatatt tctggtcatt cacctcaatt
     1321 aatat
//
LOCUS       DAPGRN010000285         1323 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPGRN010000285 DAPGRN010000000
VERSION     DAPGRN010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.291"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..764)
                     /locus_tag="RHW33_004830"
     CDS             complement(<1..764)
                     /locus_tag="RHW33_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HDV1648234.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNG"
     gene            complement(764..1126)
                     /gene="trbJ"
                     /locus_tag="RHW33_004831"
     CDS             complement(764..1126)
                     /gene="trbJ"
                     /locus_tag="RHW33_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HDV1648235.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            complement(1056..>1323)
                     /locus_tag="RHW33_004832"
     CDS             complement(1056..>1323)
                     /locus_tag="RHW33_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HDV1648236.1"
                     /translation="LAQQYGFSVFPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVAT
                     PTTFLVNVNTLEALPLLQGATDAAGFMARMDTVLQMYGGKKGAK"
BASE COUNT          325 a          369 c          325 g          304 t
ORIGIN      
        1 tcgccgttag gaccaaacac cagtgagccg gtcagcgtca tcacaaactc tttcagctct
       61 ttgttgccgt caaacattct gtttttggaa agcgcattcc acatgatgtt gatgtttttg
      121 gtcacccgct ccttatcctt gtcgctggct ttatccctga cactgtcaga tttcccgcca
      181 acggtacatc cctgccggga agccgcccag tcagcaaaaa tattgctctc accggcaatg
      241 tcctgacaga ctttctgctg ggacacctgc gtccggggga aaagcccgcc gataatcccc
      301 tgtgccgcct ggcaggaact gaggttcata ctgttaatgt cgcttgccat tttctgcagg
      361 aagtcttttg cggttttgat ttccggcacc gttgtctgca gggcaaggtc aaaaaagtaa
      421 ccggcagcat tgctcataat ctgcttaaca aaacgctgca actgctcgcc attaataaaa
      481 ctgaacgagc cgaggtaggc gtcgatgccg ccacatccgg cattgatatc cggcagcgtc
      541 atggaaatca gctgaacatt tttgacctga gtacgggcat acagggagcc accgtaagca
      601 taaccggcgg cctgcccctg ccagacgccc ggctgtgtgg tattggaggc gaagcccagc
      661 ttgttaaaga actgattcat gtcgctgttc acatccgctg acgctgcggg tgtgaccgtc
      721 agtaaagcag cacaaagaac cagaagaggt ttaatgcgtg gcatcattct tgccctccct
      781 taatttgttc tcccggcgca tacgacattt cagcaggaat atcccgatat ttccgtccag
      841 tcctgcggct gtaatatatt tcagacaccg aaatccatcc cacaattgcc cccacaaaaa
      901 acacaacaga aacagaaaag gtaaggcaca gcgaaacgac aatttctaca gggctggcgg
      961 gataaaaaag cccctgatgg ataaagggga tattcaggct gacgattatt cccgtcgcga
     1021 tgccggatat tccggcaatg agtaacacca cctgcttatt tcgcaccttt tttccctccg
     1081 tacatctgca gaacggtatc catccgcgcc ataaaaccgg cagcatccgt tgccccctgt
     1141 aaaagcggta atgcctcaag cgtgttgacg ttgaccagga aggtggtcgg tgtggccacc
     1201 gggatattcg ggaagaaggt ctgcatcacg tccggtggca ccggcaatgc ctccggaaag
     1261 gcggtatccc cctgaccatc cagggtgtag ggaaaaacgg aaaagccgta ctgctgtgcc
     1321 agc
//
LOCUS       DAPGRN010000286         1322 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPGRN010000286 DAPGRN010000000
VERSION     DAPGRN010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.292"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1219
                     /locus_tag="RHW33_004833"
                     /pseudo
     CDS             1..1219
                     /locus_tag="RHW33_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
BASE COUNT          334 a          330 c          373 g          285 t
ORIGIN      
        1 accgcctgga gcctgaagaa agctgttgcc cggagtgtgg cggtgagctt gattatctgg
       61 gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcacag
      121 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcaccatccc
      181 gtccgataga gcgtggtatc gcgggcccgg ggttacttgc ccgcgtgtta acgggaaaat
      241 actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgccag ggtgtcgaac
      301 tgagccgtgc cttactctcc aactgggttg atgcgtgctg ccagttaatg acgccgctga
      361 atgatgccct gtacagttat gtgatgaaca cccgcaaggt tcacactgat gacacaccag
      421 taaaagtact ggcaccgggc aggaagaagg cgaaaacagg atatatctgg acgtatgtcc
      481 gggatgaccg aaatgccggt tcgccagaac ctccggcggt ctggttcgcc tactcaccgg
      541 accatcaggg taaacatccg gagcaacacc ttcgtccctt ccgggtatcc tgcaggcaga
      601 tgcgttcgca ggttacgatc ggctgttcag tgccgaacgt gaaggcggcg cgttgacgga
      661 agcaggatgc tgggctcatg cgcggcgcaa aatccacgat gtatatatca gtaccaaaag
      721 cgcgacggcg gaagaagcac tgaaactaat cggcgaactg tacgccattg agcacgaaat
      781 acgcgggttg ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta aaccgctact
      841 gacttccctg tataagctga tgcaggagaa agaacacacg ttatcgaaaa aatgccgtct
      901 gagagatgcg ttccggtata tcaggaagca ctgggttgcg ttgtgcaact tctgtgatga
      961 cggtctggcg gaggcggaca ataacacagc ggaaagagcg cttcgtgcag tctgtctcgg
     1021 aaagaaaaat tacgtgttct tcggtagcga tcacggcggc gagcgtggtg cactgttgta
     1081 cgggctgatc ggcacctgcc ggttgaatgg tatcgatccg gaagcgtatc tgcgccatat
     1141 cctgagcgta ctgccggaat ggccttccaa ccgagttgac gaactcctgc catggaacgt
     1201 agtactcact aataaataag cgtcaatacg gtgctccgtt gacgcttact ataggagcgt
     1261 aaattaccta ctatcaaata cttactccaa tagcatttct gtctattcta tttcaattct
     1321 tt
//
LOCUS       DAPGRN010000287         1268 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPGRN010000287 DAPGRN010000000
VERSION     DAPGRN010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.293"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..545)
                     /gene="traF"
                     /locus_tag="RHW33_004834"
     CDS             complement(<1..545)
                     /gene="traF"
                     /locus_tag="RHW33_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HDV1648237.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDG"
     gene            complement(538..795)
                     /gene="trbE"
                     /locus_tag="RHW33_004835"
     CDS             complement(538..795)
                     /gene="trbE"
                     /locus_tag="RHW33_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HDV1648238.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(822..>1268)
                     /locus_tag="RHW33_004836"
     CDS             complement(822..>1268)
                     /locus_tag="RHW33_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HDV1648239.1"
                     /translation="KAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVG
                     EFCSKKVLGVCLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDEL
                     QKIQFNRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
BASE COUNT          293 a          346 c          281 g          348 t
ORIGIN      
        1 ccatccacgg aaacgggaat aacactcaga ccatacgtat cccggaagcc attaatgacc
       61 tgcgccagtt gcccgtcgat ggggtcctgc ccccggtaaa aaaacatgat gccgtagtgt
      121 tcagccagtt tcgcaatggc ctgtcgctgc tgcgcctggt ctgctgccag ctggttccgt
      181 accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcaggatg tgccagcatg
      241 gcctttctgg cgctcatggt gaaaagcccg gcctgctgag tccagtaatt ttgcagccgg
      301 aaatatttca caaaattatc cacgccggga tacagaatgg cttcgtacag cgcccgcttt
      361 gttgccgtct gcagtgcggc cagtttctgc ataatatccg gttcctgacg gggggctgca
      421 ggtacaggtt tattttcttt ttccttcgga tttattttct cgttatacca ctgccagcct
      481 gcatcttttc ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta
      541 ttcatcttca ttcctttcat cactttttct ttcatcaggt ttctgcaggt cagtcagggc
      601 agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaggataa cgaaagcagc
      661 ggcaagcatc ccgtctgccg tggtttcttt taccgtatcc cacgaccaga taattaccgg
      721 agaaatgaca atcgctgtaa tcaaaagccg gataagaaaa tcaataaaac ggttcgacgt
      781 gaatatgact ttcatcattt cccccggtta tttcccatac cttactgtcc tgcctgtttc
      841 agctggtcag caatctgctc tttcactttc tgcgtcagaa cgccactgtc cgggattttc
      901 tggttattca tcaggtcttc gtagaagtta gtgaaatcca gtctgttgaa ctgaattttc
      961 tgcagctcat caaccgtaat cccccgacag tcaggatgct tcgcactacc aaaactgata
     1021 cgcaactgac cgttgcgccc ctgctgctgg actatctgcg ccagcttcga atcaaactga
     1081 cagtagctgc gttttttctc cagacagaca cccagcactt tcttcgaaca gaactccccg
     1141 acgctgacgg tcagtttgtt tgattttgct ttagccaggg ccttttcatc actgctgcat
     1201 ttggccagcc cgatatcctg tccccagccg ctgtccttac agcagttgct gtatccggct
     1261 gcagcctt
//
LOCUS       DAPGRN010000288         1252 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPGRN010000288 DAPGRN010000000
VERSION     DAPGRN010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.294"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1252
                     /locus_tag="RHW33_004837"
     CDS             <1..>1252
                     /locus_tag="RHW33_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDV1648240.1"
                     /translation="TSYAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKD
                     NNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQR
                     AESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAG
                     KTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQ
                     SAERVRDQLSVMASPNGNLDEVHEGLLLQAVRASWLAKKNKARIDDVVDFLKNARDND
                     QYVESPTIRSRLDEMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPS
                     LLVAVMFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRH
                     TGAYITITQNIVDFDSDKASSAARA"
BASE COUNT          288 a          329 c          359 g          276 t
ORIGIN      
        1 agacctccta tgccaaatgg tttccgtccg tggagaaaga ggcgaaggag tggggggaac
       61 tgcgtcagcg gctgggctcc ggtcagtcct ccgtggtgtc ctactttctc aacatcacag
      121 cattctgcaa ggacaataat gaaacggcgc tggaagtgga gcaggacatc ctgaacagct
      181 ttcgtaaaaa cggttttgag ctgatttcac cgcgctttaa ccacatgcgc aatttcctga
      241 cctgcctgcc ctttatggcc gggaaagggc tgtttaaaca gctgaaagag gccggtgtgg
      301 tacagcgcgc agagagcttt aatgtggcca acctgatgcc gttagtggcg gataaccccc
      361 tgacaccggc aggtctgctg gcacccacct accgtaacca gctggcgttt atcgatattt
      421 tcttccgggg gatgaataac accaactaca acatggcggt ctgtggcacc tccggggccg
      481 gtaaaaccgg gctgatacag ccacttatcc gcagcgtgct ggactccggg ggctttgccg
      541 tggtgttcga catgggggat ggctacaagt ccctgtgtga gaacatgggg ggcgtatatc
      601 tggacggtga aaccctgcgt tttaacccgt ttgcgaacat caccgatatt gaccagtcag
      661 cggagcgtgt ccgcgaccag ttgtcggtga tggccagccc caacggtaac ctggatgaag
      721 tgcatgaagg tctgctgctg caggcagtca gggcatcatg gctggctaaa aagaataagg
      781 cgcgcatcga tgatgtggtg gatttcctga aaaacgcccg tgataacgac cagtatgtag
      841 agtcaccgac tatccgcagc cgtctggacg aaatgattgt gctgcttgac cagtacactg
      901 ccaacggcac ttatggccag tatttcaact ccgatgagcc gtccctgcgg gatgatgcga
      961 aaatggtggt gctggagctg ggcggactgg aagaccgtcc gtccctgctg gttgcggtga
     1021 tgttctccct gattatctac atcgagaacc ggatgtaccg cacgccgcgt aacctcaaga
     1081 aactgaacgt cattgatgaa ggctggcgtc tgctggactt caaaaatcac aaggtcggtg
     1141 aatttattga gaaaggctac cgtaccgccc gccgtcatac cggtgcctat atcaccatca
     1201 cacagaacat cgtcgacttt gactctgaca aggcctccag tgcggcccgt gc
//
LOCUS       DAPGRN010000289         1204 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPGRN010000289 DAPGRN010000000
VERSION     DAPGRN010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.295"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..229)
                     /locus_tag="RHW33_004838"
     CDS             complement(<1..229)
                     /locus_tag="RHW33_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HDV1648241.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGSVVVATV"
     gene            complement(240..806)
                     /gene="traE"
                     /locus_tag="RHW33_004839"
     CDS             complement(240..806)
                     /gene="traE"
                     /locus_tag="RHW33_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HDV1648242.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(828..1139)
                     /gene="traL"
                     /locus_tag="RHW33_004840"
     CDS             complement(828..1139)
                     /gene="traL"
                     /locus_tag="RHW33_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HDV1648243.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
BASE COUNT          321 a          301 c          285 g          297 t
ORIGIN      
        1 tgacggtggc caccaccaca ctgccggagg cggtctgctc attattggtc agggcaccat
       61 ccagactgtt cacggcgata atccggtcac cgggaacggt aaacagattc gggctggtat
      121 tgctgatggc aatactggcc tgaccaccgt tcaccattgg caccacggtg ggggccagcg
      181 taccgtttgc ggccatcacg ctgcaggaaa aaaacaggct gccgaatatt attgccgtat
      241 tattttttct catcgtctgt ttccccgaaa ttatccagcc aggtcacccc gttttcccgc
      301 ttcagaataa ggatgtaatg tttgatatca gtgaaaggtt ttgaatcacc aatccaggtt
      361 ttaagcacac cacgaatttc cacacggcca tactgaggcc agacacgaac actggtctgg
      421 aaaaaggctg agttcacgtt atcgttttta atacgcttcg cttcttcagc cagaataact
      481 ttcatctggt tctgtgcgcc cgggcggata tattgcagaa gcgcctgatg tgaggcatcg
      541 acggtttctg atgaaacatt cagacggagg gcaataaatg acagcgccat ctgctgtaaa
      601 taagaggcgt cggcactgtt ctgtgacacg gcaaaggggg cattaaatgc cattggcgtc
      661 acggcagtgc gttgctcatt ctgaagacgg tagttattca ctccctgaat gacgttaaca
      721 gagaggctga gaacaataag cactgacata aatataaagg cgatggccat tacacgactg
      781 gtacttaaac gggcaccgtg ttccatataa caatcctggt atcagttcta tttaatccac
      841 tgccggaaac acgaatcggg aacattatga aaaataccgc gcaacagggc tgttggcata
      901 taccagtaaa tcaggtcacg taaccaggaa ctgccccgcc cttttttcag ttttttaatc
      961 ccgaaataaa ccagaaccgc tgcaccaata ccgaacagat atttcgatgt tgtgataccc
     1021 cagccaatac agattgctgc ggggatcagt tcatccagtg gcaggccaaa ccagcggctc
     1081 tggttggtca gtgtttccgg gaaacgatat ttcttaagtt tattctcgtc tcccgacatc
     1141 gttttatttc ctgtcagagg ccaacgacgg ccatacccac agcaataaat acagagatga
     1201 tggc
//
LOCUS       DAPGRN010000290         1198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPGRN010000290 DAPGRN010000000
VERSION     DAPGRN010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.296"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1198
                     /gene="eilA"
                     /locus_tag="RHW33_004841"
     CDS             <1..>1198
                     /gene="eilA"
                     /locus_tag="RHW33_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator EilA"
                     /protein_id="HDV1648244.1"
                     /translation="TQQYIFGDFVLKNNGILLFKNKEYHIPPKELGVIILLLNADGEI
                     VSKEEIIDKVWSASVASDESLTRCIYALRKLLHENKQCKYIETVYGRGYRFTVPIVIV
                     TDNEPVKSTTTLAVFPFRTEGSINVLKLHYELVQGLSKYAFCGLDILPASVTNEASDF
                     STIHQFINKTGPEYYIMGQVVHYGKNWRLFIELIHARTHKLIDHQSIDFNPDNPLSVL
                     LSQVINILIEKIPNINFKSINTQQMPSLDSAVMYMNGRMEMYRYTPESLKRALVIFRD
                     CVSTQPQNTLPYCCLAECHISLALLGISEQKQAITTAVTSIEKALEINPSNSQALGLL
                     GLISGLKDEHSVSNVLFKQAHLLKPNSPDVYYYQSLLSFLNGDLARAFNLIEKSIALE
                     PNKMGIS"
BASE COUNT          388 a          224 c          213 g          373 t
ORIGIN      
        1 tacacaacaa tatatatttg gtgactttgt attaaaaaac aatggaatat tattattcaa
       61 aaacaaagaa tatcatattc cacccaaaga acttggcgtt atcattctat tacttaatgc
      121 tgacggtgaa attgttagca aagaagagat tatcgataag gtctggagcg cgagtgttgc
      181 tagtgatgaa tctttaacac ggtgtattta tgcattgcgt aaacttttgc atgagaataa
      241 acagtgtaag tatatcgaaa cggtctatgg tcgtggatac cgttttactg tccccattgt
      301 gattgtaacc gataatgaac cggtaaagtc gaccaccact cttgccgttt ttccctttcg
      361 tactgaaggt tcgatcaatg ttctaaaact ccattacgaa ctggtccagg gcctttcaaa
      421 atatgctttt tgtggtttag atattttacc cgcttcggta acgaatgaag ccagtgattt
      481 ctccactatc catcagttta tcaataaaac aggcccagaa tattatatta tgggtcaggt
      541 cgttcactat ggtaaaaact ggcgtttatt tattgaatta atccatgcca gaacgcataa
      601 attgattgat catcaaagta ttgatttcaa ccccgacaat cctctttccg tgctattatc
      661 acaagtaatt aatattctta ttgaaaaaat acccaacatt aattttaaat ccattaatac
      721 acaacaaatg ccatctctgg attctgctgt tatgtatatg aatggcagga tggaaatgta
      781 ccgttataca ccagaaagtt tgaaacgagc attagtaata tttagagatt gtgtgagtac
      841 gcaaccgcaa aataccttgc cctactgttg cctggcggaa tgccatatat cattagcact
      901 ccttggaatc agtgaacaaa aacaagcaat aactacggca gtaacctcca ttgaaaaggc
      961 tctggaaatt aacccttcaa attctcaggc attgggttta ctgggtttaa tcagtgggct
     1021 aaaagatgaa cattcagtgt ctaatgtgct atttaaacaa gcacatttac tcaaacctaa
     1081 ttcgccagac gtttattatt atcagtcgct gttaagtttt ttgaatggcg accttgcaag
     1141 agcatttaat ttaatagaaa aaagcattgc tcttgaaccc aataaaatgg gcataagc
//
LOCUS       DAPGRN010000291         1198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPGRN010000291 DAPGRN010000000
VERSION     DAPGRN010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.297"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1152
                     /gene="ygeH"
                     /locus_tag="RHW33_004842"
     CDS             <1..1152
                     /gene="ygeH"
                     /locus_tag="RHW33_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HDV1648245.1"
                     /translation="YSLRCIFEKIGYDRCIETIYRKGYRFSGQVFKTKINEDNTSDYS
                     IAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAATNFCNDHISQNSFLSRF
                     KPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIANILQTVH
                     KPERSVRLAKQDQGYKNHYLSDEMLAGKKELYEFTPESIYRAMTIFDGLQNKSDIQTL
                     KTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDITNVDGKILAIMGLITGLS
                     GQAKVSHILFEQAKIHSTDIASLYYYRALVNFHNEKIEEARICIDKSLQLEPRRRKAV
                     VIKECVDMYVPNPLKNNMKLYYKETESESHRVIIDNILKLKQLTRICMR"
BASE COUNT          432 a          215 c          201 g          350 t
ORIGIN      
        1 tattctttgc gctgcatttt tgaaaaaatt ggctatgatc gctgcataga aacaatctac
       61 cggaaaggtt atcgtttcag cgggcaggtt ttcaaaacta aaattaatga agataatact
      121 tcagactatt ccatagctat attccctttc accacttcat tgaatacact ggatccatta
      181 atacttaatc aggaattagt acaaatcatt tcaaataaaa aaatcgatgg tctctatacc
      241 tatccgatgg ctgcgacaaa tttttgtaat gaccacatat ctcaaaattc attcttgagc
      301 agattcaagc cagattattt cgttacagga agaataaacc agaataatgc agtgaacact
      361 ttatacattg agttgatcga cgctaaaaac cttttcctca tcgccagtaa tcatctccct
      421 gttgatgaac tacataatac atcacaattt attatagcta atatccttca aacggtacat
      481 aaaccagaac gatctgtaag attagctaag caggaccaag gatataagaa tcattattta
      541 tcagatgaaa tgttagccgg aaagaaagaa ctttacgagt tcacccctga aagcatttac
      601 agggccatga ctatatttga tggattacaa aataaaagtg atatacagac gctaaaaaca
      661 gaatgttatt gccttctagc ggaatgccat atgtctttgg cacttcatgg aaaaagtgaa
      721 cttgaacttg ctgctcaaaa agcattagag cttttagatt atgtatcaga cataaccaat
      781 gtcgatggaa aaattttagc tattatggga ctgataactg gtctgtctgg acaagcaaaa
      841 gtatctcata tcttatttga acaggctaag atacactcaa ctgatatagc ctctctctac
      901 tactataggg cacttgtcaa ctttcataat gaaaaaattg aagaggcaag gatttgtata
      961 gacaaatcac tacaactcga acccagaaga cgaaaagcag ttgtgataaa agaatgtgta
     1021 gatatgtatg tgcctaaccc gctcaaaaac aacatgaaac tctactataa agaaactgag
     1081 agtgaaagcc atcgagttat aattgacaac attttgaaac taaagcagtt gacgagaatt
     1141 tgtatgcgat aaatttttat tgacgcaagt tcattgttta aatgtatgca acttgtta
//
LOCUS       DAPGRN010000292         1189 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPGRN010000292 DAPGRN010000000
VERSION     DAPGRN010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.298"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..318)
                     /locus_tag="RHW33_004843"
     CDS             complement(<1..318)
                     /locus_tag="RHW33_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HDV1648246.1"
                     /translation="MNNTLLPSINIPCTLFETISLFDDYSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQ"
     gene            475..651
                     /locus_tag="RHW33_004844"
     CDS             475..651
                     /locus_tag="RHW33_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HDV1648247.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLAPYLYFKRKNWL"
     gene            complement(694..1074)
                     /locus_tag="RHW33_004845"
     CDS             complement(694..1074)
                     /locus_tag="RHW33_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HDV1648248.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     TYVAVNTNKWFYEKEVKGLNTWSL"
BASE COUNT          396 a          212 c          208 g          373 t
ORIGIN      
        1 ttgtccaaaa aaggaaaaca tttttgctag ttctttcatt tctgcaaata aaatatcagt
       61 gcattcacgt tgtgatattt ttgttccgga acttgaggca ctaaaagcaa cattaataga
      121 acctgggcca gggaaatggt atttaataag tctgtaagga tcaacttttg ctgagatatc
      181 gcttaggccg agtgagagaa aatcttgttc taccatatca ccatattgca tatcatcagc
      241 agaataatca tcaaataaac taatagtttc gaaaagtgta cagggaatat taattgatgg
      301 aagtaatgtg ttattcattt acattttcct tggttatatt taagctcaat atcaatttga
      361 gaacaaatgc tagggtaaaa aaatatttaa aaaagaagta cacttcacat tttttcatta
      421 catctctgaa gagaacgtcg ttaatgattg gtcttgctca ttgttaataa caaaatgttg
      481 aatttaccga ttaggaaact ttttcctacg cggattgcct ccagctatgg catgaacttt
      541 gagtttatgc cagaactgaa gtggagtttc ggctacccag gcgcgattat ctttatgatc
      601 ctcgcgggcc tggcaccgta tctgtacttt aagcggaaga actggttgta aaaaacgaga
      661 gcggtggttt agcctggcta agccacctgt tattcaaagg ctccaggtat ttaacccttt
      721 tacctctttc tcataaaacc atttgttcgt gttaacagca acataggttg ctacggcaat
      781 tcccagaatg ttaacgccaa ttagtgcacc aacaaataga gatagaatgc caagtaatag
      841 aactataatt gcttttttcc ataatcccag aacaaataaa tagatccagg aacagaagaa
      901 agcaatgaaa ttcatttgaa tagttaatcg ttgtcttact tttaatgctt taaatgctgc
      961 tttatattct ggtgttgctc cccagaatcc aggaaagcca tgttgatcat aaaaattaaa
     1021 tcggtatttc catttttcac ttaatgaacc atcgttcata tattccttac tcataaatac
     1081 tccataacta ttgttttgat gaatcagtag gtgcaagcat tagcatactg aaagtggaaa
     1141 aataacaaat taaaaaaatc atcgaaccat tgcctgaaca ggcaaaatc
//
LOCUS       DAPGRN010000293         1171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPGRN010000293 DAPGRN010000000
VERSION     DAPGRN010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.299"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..311
                     /locus_tag="RHW33_004846"
     CDS             81..311
                     /locus_tag="RHW33_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648249.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            363..>1171
                     /locus_tag="RHW33_004847"
     CDS             363..>1171
                     /locus_tag="RHW33_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDV1648250.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAR"
BASE COUNT          293 a          327 c          334 g          217 t
ORIGIN      
        1 ggacctgctt aatccacacc gcagagactg cacgaacagt ctttacggtt tcattaatta
       61 acgtcataag aggctaaatt atgacagtaa gtattgtttc accttccgca gcggctgtaa
      121 agccccgccg tcatccccgt ttcagacgcg aagacatccc cgcgccggaa attgaccccg
      181 tgctgaaagc cttcgggcga cacattgccc ggagttttca ccgggggcgt ggtgtacaca
      241 tccccgccat gaaaaacacg gccttcgggc aggtgctccg cacactcgaa ctgaaacgcg
      301 cttttaactg atgaggtgag ccgccccgcg aggggcggca acagacggaa ggagaacatt
      361 ttatgacatt agcagcaatc accatgaccg ccccggaagc cgccagccct gtgcagatgt
      421 accgcgcgac ctactcaccg gatgacaaca aactgcgcct gtatgccgtg tcacgtcttg
      481 acccggagac gtataaaaaa gtgcatgatg ccggtttccg ctgggcacca aaacaggcgc
      541 tgtttgtcgc gccagcatgg acaccgggcc gggaagacgt gctcctctca cttgccggag
      601 agattgagga tgaagacagc acgctcgctg aacgtcagga agcacgggcg gagcggttta
      661 ccggatacag cggaaagcgg gccagtgaat ccgcacaggc acttgatgaa gtggaaagac
      721 tggccgcgat gatcccgccc ggtcagccca ttcttgtggg gcatcacagc gaacgccgcg
      781 cccgtcgtga tgcgcagcgt attgaaaacg gcatgaaacg agccgtgatg ctctttgaac
      841 gtgcggaata ctgggaagag cgggcgcggt cggcactgct tcacgcgaag tataaagaac
      901 gtccggacgt tcgctggcgt cgtatcaaaa aaatcgaagc tgatttgcgc aaggctgaaa
      961 agaccatcgc gcagtcgcag aaatatctga cgatgtggcg ggctgaatcg ctggatctga
     1021 atatggcaaa actcatcagc agtcatgacc atatcagcgc ctgtttcccg ctggatacgt
     1081 atccgcgccc ggcagaaaaa agccagtatg aaggaagtcg atcgttatgg tcggccctgg
     1141 atgatgacat catcaccacg gagcaggccc g
//
LOCUS       DAPGRN010000294         1167 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPGRN010000294 DAPGRN010000000
VERSION     DAPGRN010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.300"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            97..204
                     /locus_tag="RHW33_004848"
     CDS             97..204
                     /locus_tag="RHW33_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648251.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(257..1015)
                     /locus_tag="RHW33_004849"
     CDS             complement(257..1015)
                     /locus_tag="RHW33_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV1648252.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          325 a          282 c          271 g          289 t
ORIGIN      
        1 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt ttgttacact
       61 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
      121 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
      181 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaaa gcagccagac cgcataacac
      241 gatccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat
      301 gttccggtgc ggactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
      361 caaaatgttc attaatagca acatttaatg cactttcgga gttcagcgtt gtgtcttccg
      421 cgccaaagac ggaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
      481 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
      541 gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa aactgacgac
      601 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
      661 ttgcctgacc aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg
      721 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
      781 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
      841 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
      901 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
      961 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     1021 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     1081 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     1141 ttcttatttc attttttgaa taagcat
//
LOCUS       DAPGRN010000295         1142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPGRN010000295 DAPGRN010000000
VERSION     DAPGRN010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.301"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1142
                     /locus_tag="RHW33_004850"
     CDS             <1..>1142
                     /locus_tag="RHW33_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDV1648253.1"
                     /translation="RVCVQSPSFMPDLDGEKNKTELFVDDIIAYLKSPSVYSLEKAGS
                     LSDFVNNSSEIEPWVFIGGGLIIIVSASEQDPESMIVTVGDVEGKYATQIFVPPALTT
                     SLKDIFIRSHTNAKYECFTKEQIKANIKSNVINYNCKLHFNEKPENEIVCRHLSLQYC
                     IDSMNEHTGKVPLKAYYSSPEDIQKHIPFELEQRFDNLQKNPPPGTCVVASDKFGEAL
                     SVFFHRMEKEKLTHMAAIVQSQTHAMAVRLRIKKTPAGETEYVVSFYDPNATNTAVRY
                     KANNCDSFGTLQSFMNIKQAQQKWVMTEICSDCVGITPYLPREQAHLLSGINNELQPP
                     LSPSALLLLMRMGMHENVVLFFDKFKNSQEMTASNVLAAKSLTGTY"
BASE COUNT          382 a          206 c          212 g          342 t
ORIGIN      
        1 cagagtatgt gtacaatctc cttctttcat gcctgatttg gatggagaaa aaaataaaac
       61 tgaattgttc gttgacgata ttattgcgta tcttaaaagt ccttcagttt attcacttga
      121 aaaagcgggt tcattaagtg attttgttaa taatagcagt gagattgaac catgggtttt
      181 tatcggtggt ggacttatta ttattgtctc cgcatctgag caagaccctg aaagtatgat
      241 tgtaactgtg ggggatgttg aaggaaagta tgctacacag attttcgtac cacctgcgct
      301 tacaacatcc ctcaaagata tttttattcg ctctcatacg aacgcaaagt atgagtgttt
      361 tacaaaagag cagataaaag ccaacattaa atctaatgtt ataaattata attgtaaatt
      421 gcattttaat gaaaagccag aaaatgagat cgtgtgcagg catctttctc tacaatattg
      481 tatagattca atgaatgaac atacagggaa agttccatta aaggcttact attcatctcc
      541 ggaagatata caaaaacata ttccattcga gcttgagcag cgatttgaca atctacaaaa
      601 aaatccacca cccggtacat gcgttgttgc cagtgataag tttggggagg cattatcagt
      661 cttttttcac agaatggaaa aagagaagtt aacgcatatg gcggcaatcg ttcaatctca
      721 aacacacgct atggccgtcc gcttgaggat caaaaaaacg cctgctggtg aaacagaata
      781 tgttgtatcc ttttatgatc ctaatgcaac caatactgca gtacgctata aagcaaacaa
      841 ctgtgattct tttggaacat tgcaatcctt tatgaatatt aaacaggcac aacaaaaatg
      901 ggtaatgaca gaaatttgct ccgactgtgt aggaataact ccttatctcc ctcgagaaca
      961 agcccattta ttaagtggta taaataatga gttgcaacca ccattatcac catcagcatt
     1021 acttctatta atgagaatgg ggatgcatga aaacgttgtc ctttttttcg ataaatttaa
     1081 aaactctcaa gaaatgacag catcaaatgt tcttgctgcg aaatcactta caggaactta
     1141 tg
//
LOCUS       DAPGRN010000296         1138 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPGRN010000296 DAPGRN010000000
VERSION     DAPGRN010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.302"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1138
                     /locus_tag="RHW33_004851"
     CDS             <1..>1138
                     /locus_tag="RHW33_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HDV1648254.1"
                     /translation="YHEGNAFVIMGAGEQLKRIKYDVDENNLKVFNVHFDNNEVLVTD
                     GEPDVVCLSKQVWENLLIKLKPEIKENAASEVHKSANKGEIEQLVECSKRNEQTLFDN
                     IRKSDFHVGSLKPGSMNSVILEMPPNVCMEPRNPYENKIDEVSSLSEPKEHTIDIQEK
                     TDAFVNEFKGILFDQNGRSSEFLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFCS
                     STLTHNNIGFDFKLFPEVKLSGEHLETVFKYKNGDDVREIAKINITLQKEEGGLYNLR
                     GLDFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLERVSENGL
                     SGVLSDRNKKIKLITGVAPFDDILFMDDDFDDNSPEDGPVENSPV"
BASE COUNT          387 a          168 c          233 g          350 t
ORIGIN      
        1 tatcatgaag gcaacgcctt tgtcattatg ggagctggag aacaattaaa acgtattaaa
       61 tatgatgttg atgaaaataa cttaaaggta ttcaacgtac actttgataa taatgaagtg
      121 ttagttactg atggtgagcc tgacgtagta tgtttaagca agcaggtctg ggaaaatctt
      181 ctcattaaat taaaaccgga gatcaaggaa aatgcggctt ctgaagttca taaatcagcg
      241 aataaaggtg agattgagca attagttgaa tgctctaaaa gaaatgaaca gacccttttc
      301 gataatataa gaaaaagtga ttttcatgtt ggttcactta agccaggtag tatgaatagt
      361 gttattttag aaatgccacc aaatgtctgt atggaaccac gtaatccata tgaaaacaaa
      421 atagatgagg tttcatcttt gtcagagcca aaggaacaca ccatagatat tcaagaaaaa
      481 acagatgcgt ttgtgaatga gttcaagggg atattatttg atcaaaatgg aaggtcttca
      541 gagtttctgt ttaattttta tgaatgttgc tataagtttt taccaagagc gcagcctcag
      601 gataaaatcg aaagctataa ttcagcactg caagcttttt ccatcttttg ttcatctacg
      661 ttgacacata ataatatagg ctttgatttc aaattatttc ctgaagtcaa actgtctgga
      721 gaacatcttg aaacggtatt caaatacaaa aatggcgatg atgtccggga gatagccaaa
      781 attaacatta ctctccaaaa agaagaaggt ggtttatata atttacgtgg attggatttt
      841 aagggatgct tcttttctgg acagaacttc agtaactatg atattcaata tgtgaactgg
      901 ggaacgtcat tgtttgatct tgatactcca tgtattttta atgcgcctgc ttacaacaag
      961 agtaatgaaa aatcattaga acgcgtcagc gaaaacggtt taagtggagt cttgtctgat
     1021 cgtaataaaa aaataaaact aatcacgggt gtggcaccat tcgatgatat tttatttatg
     1081 gatgatgact ttgatgataa ctcccctgag gatggtcccg ttgagaatag tcctgttg
//
LOCUS       DAPGRN010000297         1133 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPGRN010000297 DAPGRN010000000
VERSION     DAPGRN010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.303"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..438
                     /locus_tag="RHW33_004852"
     CDS             55..438
                     /locus_tag="RHW33_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HDV1648255.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLEYVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            625..>1133
                     /gene="traJ"
                     /locus_tag="RHW33_004853"
     CDS             625..>1133
                     /gene="traJ"
                     /locus_tag="RHW33_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HDV1648256.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWT
                     ISVQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTG"
BASE COUNT          376 a          172 c          233 g          352 t
ORIGIN      
        1 aattttagtg tttcgaaatt aactttattt tatgttcaaa aaaggtaatc tctaatggct
       61 aaggtgaacc tgtatatcag caatgatgcc tatgaaaaaa taaatgcgat tattgagaag
      121 cgtcgacagg aaggggcaag ggaaaaagat gtcagttttt cagcaacagc ttcaatgctt
      181 cttgaactgg ggcttcgtgt acatgaggct cagatggagc gtaaagagtc tgcatttaat
      241 cagactgagt ttaataaatt gcttcttgaa tacgttgtaa aaacacaatc atcagtagcg
      301 aaaattttgg gtattgagtc tctcagtcct catgtctccg gaaatccaaa gtttgaatat
      361 gccaatatgg ttgaagatat cagggagaag gtatcatctg agatggaacg attttttcca
      421 aaaaatgatg atgaataaaa gaaaattgac ttcgttcaaa tatcagagtt tttatgattt
      481 aaaaaggtga cagtacgaaa gataattagt atattaatta cgtggttaat gccacgttaa
      541 aatttgaaat tgaaaatcgc cgatgcaggg agacgtgaac tccctgcatc gactgtccat
      601 agaatccttt gtgaggaggt tcctatgtat ccgatggatc gtattcaaca aaaacatgct
      661 cgtcaaatag atctgctgga aaatctgacg gcagttattc aggattatcc aaatccagcc
      721 tgtatcaggg acgaaactgg aaaatttatt ttttgcaata cgctgtttca tgagtcattt
      781 cttacacaag atcaaagtgc tgaaaaatgg cttctgtcgc agagagattt ttgtgaattg
      841 atctctgtca cagagatgga agcatatagg aatgagcata cgcatcttaa tcttgtagag
      901 gatgttttta ttcagaacag attctggaca atttctgtcc agtcatttct taatggacac
      961 agaaatatta ttctgtggca attttatgat gctgctcatg ttcgtcataa agacagttat
     1021 aatcaaaaaa cgattgtcag tgatgatatc agaaatataa tcagaagaat gagtgatgat
     1081 tcttctgtat catcatatgt aaatgatgtc ttttacttat atagcaccgg aat
//
LOCUS       DAPGRN010000298         1130 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPGRN010000298 DAPGRN010000000
VERSION     DAPGRN010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.304"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..259)
                     /locus_tag="RHW33_004854"
     CDS             complement(<1..259)
                     /locus_tag="RHW33_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648257.1"
                     /translation="MAKNIFTEFPTYPVDQLSGIFINGISPESMTHDFEAKRVKHKQY
                     KECIRDHEKGTVFCVATLAKRPKYRFRVGQEVDVVNPYSFNC"
     gene            complement(298..591)
                     /locus_tag="RHW33_004855"
     CDS             complement(298..591)
                     /locus_tag="RHW33_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648258.1"
                     /translation="MMRNLENGNFIKVENKEGTFIMKKYKPKKSRTKRARNGSAVAKR
                     RLEKRIQKQSKRIVAVKSDKISTRYSTLGSWESNEEMMLKNINKHIKSRMRYV"
BASE COUNT          276 a          265 c          224 g          365 t
ORIGIN      
        1 gacagttaaa gctataagga ttaactacat cgacttcttg ccctacacga aaacgatact
       61 taggacgttt agccaatgta gcgacacaaa acacggtccc tttttcatga tcgcggatac
      121 attctttata ttgtttatgt ttaactctct tcgcctcaaa atcatgtgtc atagattctg
      181 ggctaatgcc attgataaaa ataccggaaa gctggtcgac cggataagta ggaaattcag
      241 taaaaatatt tttagccatg ttataccccc ataagattct atgagggtat ttatggatta
      301 aacgtatctc atgcgcgatt taatgtgctt attaatgttc ttaagcatca tttcctcgtt
      361 gctttcccac gatcctagcg tcgaataacg agtagaaatt ttatccgatt taacggcaac
      421 aattcgcttg ctctgtttct gtatgcgttt ctctaagcga cgttttgcca ccgccgatcc
      481 attcctagcg cgtttggttc gggatttctt aggcttgtac ttcttcatga taaaagtccc
      541 ttccttgttc tctaccttga tgaagttgcc gttttcgagg ttacgcatca tcttaccgcc
      601 tttgatggcc ttaatgttcc cgtgggagtc taagacctta ccgttggcaa ttggcactag
      661 cttattgatt ttgccaccat cgaaatagtg tttcagatag tcatcctgac tgccgatctt
      721 cttgtcctta tcccttgcgc ccttaatgaa gatttggtgt ctcgtccctg aacggttctt
      781 gtagttggta ttacctacag cttgctgagt ccagcgagta ggtctatcaa cattcttgtt
      841 aatcttcttc tggagtgcac gagcagcaaa gatcgcccct tcacctatcg ccctttcgaa
      901 tgtatcggtt gccttttcag accattgcct caacgctttc ttagtcctta gagcgtttcg
      961 tttattggta ctggttgcca tgttagcccc ctaataacga aaagaccccc gccagtgtcc
     1021 ttagcaattg ttcatccacg gtcatagccg ggagttcaag cccaaaggac gttagcagag
     1081 gtctaattgc tacgttataa gtgagggcga caatcaagag ataaccaaaa
//
LOCUS       DAPGRN010000299         1110 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPGRN010000299 DAPGRN010000000
VERSION     DAPGRN010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.305"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..334
                     /locus_tag="RHW33_004856"
     CDS             95..334
                     /locus_tag="RHW33_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
                     /protein_id="HDV1648259.1"
                     /translation="MADWNGYIMDISKQFDQGVDDLNQQVEKALEDLATNPSDPKFLA
                     EYQSALAEYTLYRNAQSNVVKAYKDLDSAIIQNFR"
     gene            354..686
                     /gene="sctI"
                     /locus_tag="RHW33_004857"
     CDS             354..686
                     /gene="sctI"
                     /locus_tag="RHW33_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV1648260.1"
                     /translation="MSVSNMPPIDRVEQSTAHEIQQAKVIDLNDRVLNLDNPDDKMIS
                     AFANYAVQTENWQQNALQALRSDKKGLTPEKLLVLQDHVLNYNVEVSLVGTLARKIVA
                     AVETLTRS"
     gene            683..>1110
                     /gene="sctJ"
                     /locus_tag="RHW33_004858"
     CDS             683..>1110
                     /gene="sctJ"
                     /locus_tag="RHW33_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HDV1648261.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIVSSRVHVSYDVDTGDSGKTALP"
BASE COUNT          355 a          197 c          240 g          318 t
ORIGIN      
        1 ggatacaaat aatagttcat ttagctatgg agataaccga ttcgagaagt ctcaaggtag
       61 caagtggacg tttcaggaat aattgggagg acttatggca gattggaatg gttatattat
      121 ggatatcagc aaacaatttg atcagggcgt tgatgatctg aaccagcaag ttgaaaaagc
      181 gttggaggat ttagcaacca atccctccga cccgaaattc cttgccgaat atcaaagtgc
      241 attagctgag tatacattgt atcgaaatgc gcaatctaac gttgtaaaag cgtataaaga
      301 tcttgattcg gcaatcatac aaaacttccg ctaaactgga taaggatttt attatgtcag
      361 taagcaatat gccacctatt gatagagttg agcaatcaac tgctcatgaa atacagcaag
      421 cgaaagtgat tgatttaaat gaccgggttc tcaatcttga caacccggat gataaaatga
      481 tctccgcttt tgccaactat gctgtacaaa ctgaaaattg gcagcaaaat gcgttacaag
      541 cattaaggtc tgataaaaaa gggttaactc ctgaaaaatt attagtcttg caggatcatg
      601 tgcttaatta taatgtcgag gtttcattgg tgggaacatt agcaagaaag attgtcgctg
      661 cagttgagac gttgacccgc tcatgaaata tatatcatta ttattgttta tcctgctgtt
      721 gtgcggctgc aagcagcagg aattactgaa ccatcttgat caacagcaag ctaatgatgt
      781 ccttgctgtt ttacaaagac ataatattaa tgctgaaaag aaggatcaag gcaaaactgg
      841 attttcaata tttgtcgaac caacggattt tgcttctgcg gtggattggc taaaaatcta
      901 caatctcccg ggtaagcctg atattcaaat atcgcagatg ttccctgcag atgcactcgt
      961 ttcatctcca cgagcggaaa aagcaaggct atattctgct atagagcagc ggttagagca
     1021 atctctcaaa attatggatg gcattgtttc ttcgagagta catgttagtt acgatgttga
     1081 tactggcgat agtggtaaaa ctgctttacc
//
LOCUS       DAPGRN010000300         1106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPGRN010000300 DAPGRN010000000
VERSION     DAPGRN010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.306"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            89..391
                     /locus_tag="RHW33_004859"
     CDS             89..391
                     /locus_tag="RHW33_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1648262.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            438..860
                     /locus_tag="RHW33_004860"
     CDS             438..860
                     /locus_tag="RHW33_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV1648263.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            857..1048
                     /locus_tag="RHW33_004861"
     CDS             857..1048
                     /locus_tag="RHW33_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648264.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
BASE COUNT          253 a          271 c          350 g          232 t
ORIGIN      
        1 agagtgcgac cgtctctcct ttctaccgta tctgtttggc aacgattttc tgtatgccga
       61 ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa tatcagggag gcttctggca
      121 cttcatccgc ctgccggacg gtggcggtta catgatgccg gatggcgacc gtttccacat
      181 ggtgaacggt gcaaactggt ttgaccgtac cgtgagtgct gatgccgcag gcatcattct
      241 gacttccctt gtgattaacc gccagttgtg gctgtaccac gacagcgggg atgcaggact
      301 gacccagctt taccggatgc gcgatgcgca gttgtggcgt cacatcgaat ttcaccccga
      361 atgcaacgcg atttacgcgg cactggactg attaacggac ggggcggcaa tgccgcccct
      421 gtaaaggaga cgagaacatg tactgtactg ttaaagaaat tatccgcgaa gtgctgaaca
      481 cagacgtgcc ggacagtgaa tgcgtttttg ccgtggtgct gacccgtggg gatgtgcgtc
      541 acatagccca ggactggagt ctgacagacg atgagctgga aaccgtcatg cagcgactgg
      601 acgatgcctt tgcgcatggt gcggatgtca gcattgttca cgacgttgtt cgtgagctga
      661 tggaagaaaa gcgcgccagc cgtcaggtga cagttccggc ggtgatgctg gaaaaagtga
      721 tggcgctggc aggcagtgaa atgaagcgcc tgtatgccgt cgggagcgag aacgggggcg
      781 acggtgacgc attcgtcagg gaagaacgcg aagcaatgga cgttgtgtta caggcgctgg
      841 acggggagca catgtcatga atatcagcac agaaacccgc gaaattctgc gcaattacaa
      901 agccgtgatt aatgcgcggc gtcgtgaaat ggggcagaaa ccgctcacca ctgcgcagat
      961 tgttgatgaa atctgcgatt ttgtggcgaa tcagcaggct gtattcctcg gcggtcacta
     1021 catccttcag ggcagcataa acaggtgata caggggcagg cggtggaggc cgcctgtcag
     1081 cccgcgcgaa aacccctggc aggctg
//
LOCUS       DAPGRN010000301         1094 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPGRN010000301 DAPGRN010000000
VERSION     DAPGRN010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.307"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(32..364)
                     /locus_tag="RHW33_004862"
     CDS             complement(32..364)
                     /locus_tag="RHW33_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648265.1"
                     /translation="MKEFTITKSLYDWTVKISGKTVSVSPSHVENDWGVSRETISRSM
                     HVPFDLMSLTGIHAMLEVREQFEAGNHNATPVNNHLLTNEEREIIENWRYEYAIPYYE
                     EDQVEIDW"
     gene            complement(485..952)
                     /locus_tag="RHW33_004863"
     CDS             complement(485..952)
                     /locus_tag="RHW33_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648266.1"
                     /translation="MNHKLTTTTPFILLGLDADSFHNDKLMKMFRERGYNILYNTDES
                     HFSSAWYVFGRNKRPTIVVNCFTDEDRLKYQNIPAIIVRDDLPFEVRDYDIDLGSFFT
                     VYELDEAMFNYGKETVQEAPEQDEEDEEDEDYAPICFSSQNGFYGYDEGLDML"
BASE COUNT          336 a          208 c          217 g          333 t
ORIGIN      
        1 ccggaacatt tttatttatg atagaaattt attaccaatc gatttctact tggtcttctt
       61 cataataagg aatcgcatat tcataacgcc aattttcgat gatttcacgt tcttcattag
      121 taagaaggtg attgttaact ggagtcgcat tgtgattacc agcttcgaat tgctcgcgaa
      181 cttccagcat tgcatgaata ccagttaagg acatcagatc aaacggaaca tgcatactac
      241 gggagatagt ttcacgggat acgccccaat cattttctac atggctaggg cttacagaaa
      301 cggttttgcc ggagatttta acagtccagt catacaggga tttagtgatg gtaaattctt
      361 tcatgttaca tcctctttac aaatctaggt caaataggtt caaagttgtc aagtgcgata
      421 atcataaaaa agtcataaaa ttttgtcaac accccttgac aagacgccaa agggttgacc
      481 taacttacag catatctaag ccttcatcgt aaccgtagaa gccattctga gaagaaaaac
      541 agattggagc gtagtcttcg tcttcctcat cttcttcatc ttgttctggg gcttcctgaa
      601 cggtttcttt gccgtagtta aacatggctt catccagttc atagacagta aagaagctac
      661 ctaaatcaat gtcataatca cggacttcga aaggcaggtc atcacgaaca atgattgctg
      721 gaatgttctg gtatttcaga cggtcttcat cggtgaagca gttaacaacg atggtaggac
      781 gtttatttct tccgaaaaca taccatgcac tagagaaatg ggattcatcg gtattgtaaa
      841 ggatgttata accacgttcg cggaacatct tcattaactt gtcattgtga aagctatctg
      901 catccagacc cagaaggata aaaggagtag tggtagtcag tttatggttc atagtggaaa
      961 tcctctaaaa gaaagtgaat caaagtaatt ctgatattat ttataagaga aaattcttga
     1021 tgattttgga agggattggt attcagtcgg gcaaagccct ccttcatgtt tttgattaaa
     1081 tcatcatcaa aacg
//
LOCUS       DAPGRN010000302         1094 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPGRN010000302 DAPGRN010000000
VERSION     DAPGRN010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.308"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..545)
                     /locus_tag="RHW33_004864"
     CDS             complement(<1..545)
                     /locus_tag="RHW33_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ref family protein"
                     /protein_id="HDV1648267.1"
                     /translation="MKTIEQKIEQHRKWQKAARERAIARQREKLADPAWRESQYQKMR
                     NTIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISEEVSLHHISGRTAPGCHKKQLPLCRWHHQHAAPAEVREKYPWLVPVHADGVVGG
                     KKEFTLLNKSEMELLADAYEMA"
     gene            complement(792..1001)
                     /locus_tag="RHW33_004865"
     CDS             complement(792..1001)
                     /locus_tag="RHW33_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HDV1648268.1"
                     /translation="MADIDSISVAFPVKSIRSAEHFVMNATEEEARRGFAKVMSEFGE
                     FLGHVDKALSISSARSKALTASMMK"
BASE COUNT          249 a          273 c          249 g          323 t
ORIGIN      
        1 gccatttcat aggcgtcagc cagcaactcc atctctgact tgttcagcaa ggtgaattct
       61 ttcttgcctc caaccacacc atcggcatga acagggacca gccaggggta tttttctctt
      121 acttcagccg gtgctgcatg ctggtggtgc catctacaaa gtggcaattg ctttttgtga
      181 caaccaggcg cggtacgacc ggagatatgg tgcagagaca cctcttcaga tattactcca
      241 tgcatatagc aggcaatgca ggggagagcg ccaagagcat tggctatgcg ccgctcctcc
      301 gccgtcggtg ttcgtccctt caagccacga gattttattt ttaccgcgct tttccgcgtt
      361 ttgctggctg gtgggcgctc tttctgttta gcgatacggc ggtcgatagt attccgcatt
      421 ttctgatatt gagattctcg ccaggccgga tcagccaact tctcccgttg ccgagcgatc
      481 gctcgttctc tggctgcctt ctgccactta cggtgctgtt caattttttg ttcgattgtt
      541 ttcatatggc aaaaaaaagg cggcctgatg gccgccaatg atgtcaagga gttaagtaat
      601 ggcaacgtct tcgtagttga caaaaactgc ggctcaatta tagcaatcaa ttagagcaat
      661 ggtagatatt ttgtttatcg cgaatcacat tttttcactt cagtacctgt gtgctatact
      721 ccttcttgat tgattggatg cggaatacaa acccgctctt ttgtgcagcc tggctccttg
      781 ccaggctttt tttatttcat catggaagct gttaatgctt tggaccttgc tgaactgatt
      841 gagagggctt tgtcaacgtg ccccaaaaat tcgccaaact cagacatcac tttggcaaaa
      901 ccgcgccgtg cttcttcctc ggtggcattc atcacgaaat gttcagcact acgaatactt
      961 ttgacaggga acgcaacaga tattgagtca atatcagcca ttctatcgct taactttaca
     1021 gtgacaatga cggctggcga ctgaatatta gtgcttacag acagcactac atattttccg
     1081 ccgatgtcga aatc
//
LOCUS       DAPGRN010000303         1091 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPGRN010000303 DAPGRN010000000
VERSION     DAPGRN010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.309"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1091
                     /locus_tag="RHW33_004866"
     CDS             <1..>1091
                     /locus_tag="RHW33_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648269.1"
                     /translation="VNDPKKMKRIFPLFIVSENINIHAEPDIEYFRVIDRAFDEVANY
                     TGRIFSYLRTHEPLKLTSCVNKQTLLSMGGYLNEWNVFDSLSRVRDFFRLSSAVFTKL
                     DNNIYSLEVDSFCLYRDYEIARNRLMMRASHLYSEVHEFSNKHFHLNSWVKDHMPSYL
                     NSDGVFSSFHLSELENMSPDDLHEEYGNISLFNWVHAYQCLVELSKEEMSKRFSSTKP
                     IPLQLDRWLIIKSRESWLSFFQRKGIAADAAKKLIDYFTFNSKSHDLNDCPFIPCMDG
                     LCLMPALIANSSVTRSLMSLFGSKKISQASKGRFHEQQFIKQVRDAGIKASPIDAHAN
                     YQCDCVILLDDCLIFTELKSNGQPIYYGK"
BASE COUNT          355 a          171 c          203 g          362 t
ORIGIN      
        1 gtcaatgatc ctaaaaaaat gaagaggatt tttccattat ttatcgtttc tgaaaatatt
       61 aatattcatg ctgaacctga tatcgaatat tttcgagtta tcgatagggc atttgatgag
      121 gtagccaatt acaccggaag aatattttct tatttacgga ctcatgagcc attgaaacta
      181 acttcttgtg tgaataaaca aaccttgctt tcaatgggag ggtaccttaa tgagtggaat
      241 gtttttgact cattaagcag agtaagagac tttttcagac taagtagtgc agtatttaca
      301 aaattggata ataacattta ctctcttgag gttgatagtt tttgtttata ccgagattat
      361 gagattgcaa gaaatagact aatgatgaga gcgtcacatc tatatagcga agttcatgaa
      421 tttagtaaca aacattttca tctaaatagt tgggtaaagg atcatatgcc atcttactta
      481 aattctgatg gcgtttttag ctcatttcat ctaagcgagt tggaaaatat gtctcctgat
      541 gaccttcatg aagaatatgg gaatatatca ttatttaatt gggtgcacgc ttatcaatgt
      601 ttagtcgagt tatctaaaga agaaatgagc aaaagatttt catcgacaaa acctataccc
      661 ctacagttgg atcgctggct gatcataaaa agccgtgaga gttggttatc attctttcaa
      721 cgtaagggga ttgcagcaga tgcagcaaaa aaactgatag attattttac cttcaattca
      781 aaatcacatg atcttaatga ttgcccattt attccctgta tggacggttt atgcttaatg
      841 cctgcattaa tagcaaatag ctcagtgact cgctctctaa tgtcattatt tggttccaaa
      901 aaaatttctc aagcaagtaa agggcgattt catgagcaac aatttataaa acaagttcgt
      961 gatgcgggga ttaaagcttc tccaattgat gctcatgcta attatcagtg cgattgcgtg
     1021 attctcctgg atgattgttt gatttttaca gaactaaaat caaatggtca accaatatat
     1081 tatggtaaat a
//
LOCUS       DAPGRN010000304         1078 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPGRN010000304 DAPGRN010000000
VERSION     DAPGRN010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.310"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..578
                     /locus_tag="RHW33_004867"
     CDS             45..578
                     /locus_tag="RHW33_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1648270.1"
                     /translation="MKKYIIPFVATTLMFSATTFAKDVDQGTLTIRGEVVDTTCYFVN
                     GDSTTDIVVEDVVTSAMNQLINNEIYTLKTGSTSHDLEIQCDGNTAPSIEVLASEFDA
                     NNFTLNKGTAENIGFAIFLDDGTLTRMRPGLKTPLKDNGSGQYFLNLNAQYARTSGNP
                     VTKGSVESTVTIKISAD"
     gene            628..>1078
                     /locus_tag="RHW33_004868"
     CDS             628..>1078
                     /locus_tag="RHW33_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV1648271.1"
                     /translation="MRIITALLMSFFILPVHAGVVIYGTRIIYPEKNREVLVQLMNQS
                     KNASLIQAWIDDGNTTIAPEKIQVPFILTPPVSRVAGGSGQQLKIRKMPNNLPHNKES
                     LFYLNVLDIPPNNPQNAGKNKIKLALQNRIKLLWRPSGIAPVDKKSLS"
BASE COUNT          362 a          213 c          205 g          298 t
ORIGIN      
        1 ttgaaaacga gtgaccttcc ataccagtta attaaggatg atttatgaaa aagtatatta
       61 ttcccttcgt tgcgacaacg ttgatgttta gcgcaacaac cttcgccaag gatgtcgatc
      121 aaggaacact aaccattaga ggagaagttg ttgatacaac gtgttacttt gttaatggtg
      181 attcgacaac agatatcgtt gtcgaagatg ttgtaaccag tgcaatgaat caacttatca
      241 ataatgaaat ctatacttta aaaaccggtt caacctctca tgacttagaa attcagtgtg
      301 acggaaatac agccccaagt atcgaagtac tagcatcaga gtttgatgct aataacttta
      361 cgctgaataa aggaactgct gagaacattg gctttgccat tttcctcgat gatgggactt
      421 tgaccagaat gagaccaggg ttaaaaacac cgctgaaaga taatggctct ggtcaatatt
      481 ttcttaattt gaatgcacaa tatgcccgca caagtggcaa tccagttaca aaaggctccg
      541 tagagtcaac cgtaacgatt aaaatcagtg cagattaacg ttttttgccg gtagctctgc
      601 taccggtatt atttacggag tgattacgtg agaataataa cagcattact gatgtcattt
      661 tttattttac cagttcatgc cggtgtggtc atctatggca cgcgcattat ttatcctgaa
      721 aaaaacagag aagttctcgt acagttaatg aatcaaagta aaaatgcctc tttaattcag
      781 gcatggatcg atgatgggaa caccactatc gcgccagaaa aaattcaggt cccatttatt
      841 ctcacaccgc cagtaagtcg tgtagcaggt ggttctggcc aacaacttaa aataagaaaa
      901 atgccaaaca acttgcctca caataaagaa agtttatttt atctaaatgt tctggatatc
      961 cctccaaaca acccacaaaa tgcaggaaaa aacaaaatta aacttgcatt gcaaaaccgt
     1021 ataaagctac tttggcgtcc aagcggaata gctccggtag ataaaaaaag cctttctc
//
LOCUS       DAPGRN010000305         1064 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPGRN010000305 DAPGRN010000000
VERSION     DAPGRN010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.311"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..455
                     /gene="trbI"
                     /locus_tag="RHW33_004869"
     CDS             69..455
                     /gene="trbI"
                     /locus_tag="RHW33_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HDV1648272.1"
                     /translation="MTTTQKTTDVTARRRSHWWWTVPGCLAMVLLNAAISYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            452..>1064
                     /gene="traW"
                     /locus_tag="RHW33_004870"
     CDS             452..>1064
                     /gene="traW"
                     /locus_tag="RHW33_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HDV1648273.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIP"
BASE COUNT          236 a          279 c          344 g          205 t
ORIGIN      
        1 aagtcagggc cggagatggc ttcgctggaa gcctggctgg aagaacatga gaaatacagg
       61 agtgttgcat gaccacaacg caaaaaacga ctgatgtgac agcccgtcga cgcagccact
      121 ggtggtggac ggtgccggga tgcctggcaa tggtgttact gaacgcagcc atcagttacg
      181 gcattgtcag actgaacgca ccggtgactg ccgccttcaa catgaaacag accgtggatg
      241 cgttttttga cagcgccagt cagaaacaac tgtcagaagc acagtcaaaa gccctttcag
      301 cgcgttttaa cacggcactg gaagccagcc tgcaggcatg gcagcagaaa caccacgcgg
      361 tcattctggt gtcgcctgcc gtggtacagg gggcaccgga tatcacccgg gaaatccagc
      421 aggatatcgc ccggcgaatg agggcggagc catgagatgc cgggggctga ttgccctgct
      481 gatatggggg cagagtgtgg ccgccgccga tcttggtacc tggggcgatc tgtggccggt
      541 aaaggagccg gacatgctga cggtgatcat gcagcgcctg acggcactgg agcagtccgg
      601 tgagatgggc cggaaaatgg atgcctttaa ggagcgggtg atccgcaaca gtctgcggcc
      661 ccctgccgtg cccggtatcg gacgcacgga gaaatacggc agccggttgt ttgacccgtc
      721 cgtcagactg gctgcggata tccgggataa cgaagggcgg gtgtttgccc gtcagggtga
      781 agtgatgaat ccgctgcagt atgttccttt taaccagacg ctgtatttca tcaatggcga
      841 tgatccggcg caggtggcct ggatgaaacg ccagacgccg cccacactgg agagcaaaat
      901 tatcctcgtg cagggcagta tcccggagat gcagaagtct ctggacagcc gtgtttactt
      961 tgaccagaac ggtgtgctct gccagcgcct cggcattgat caggtcccgg cacgggtgag
     1021 tgccgttccc ggcgatcgct tcctgaaggt ggaatttatt ccgg
//
LOCUS       DAPGRN010000306         1062 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPGRN010000306 DAPGRN010000000
VERSION     DAPGRN010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.312"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            204..>1062
                     /gene="espX2"
                     /locus_tag="RHW33_004871"
     CDS             204..>1062
                     /gene="espX2"
                     /locus_tag="RHW33_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX2"
                     /protein_id="HDV1648274.1"
                     /translation="MDCSKCNGYATMLLNMVQGSDPVNLLELHGFLEHFAYYVSFGKF
                     NAGHQRYNAFKKFVSEISEISANDINMTIKTGQSRHENVISINMNDAIPRDEKGITVR
                     IDNINGKKNNSNSSDVFIPYVNTFPDLKNKILRMKIELTEGSGFSKSLSDSQIEMHIL
                     RNVNSLNVGEKLNDDNLAYHSIFTNEFSVIIPPSYYDATSVVNANNGVREKLFESDSK
                     VKDIVDDMSNHDVESEKDIFVIGGMIEKLDSLVDESFNENTDNIQTVKDLLTQLTDGM
                     ELFALRDIVA"
BASE COUNT          353 a          169 c          213 g          327 t
ORIGIN      
        1 ttaactatca aaaatcgctc atttctgctt attgatagaa agattgattg ccagcctgtt
       61 tagatccgta cagcagcaga ctttcataac ccggttgatc ctgagtttgc agtgcgaaaa
      121 gttaaaaatg agcgatttct gtaagattcc ccattttgcc aggcttatga ttcgcagatc
      181 attcaagtgc aaaggagcga tgtatggatt gttcaaaatg caatggttat gcgacgatgt
      241 tattaaatat ggtgcagggt tctgacccgg ttaatttact ggaattacat ggtttccttg
      301 aacatttcgc atattatgtt tcatttggca aatttaatgc tggccaccag cgctataatg
      361 catttaaaaa attcgtcagt gagatttcgg agatcagcgc aaacgatatc aatatgacga
      421 ttaaaaccgg gcaatccaga catgaaaatg ttatctctat aaatatgaat gatgcaattc
      481 ctcgtgatga gaaagggatc accgtcagaa tagataatat taatggaaag aaaaataact
      541 ctaattcatc agatgttttt attccttatg taaatacatt tcccgacctc aaaaataaaa
      601 tactcaggat gaaaattgaa ctgactgaag gtagtggttt ttctaaaagt ctttctgaca
      661 gtcagattga gatgcatatt ctgaggaatg ttaattcgct aaacgttggc gagaaattga
      721 atgatgataa tcttgcatat cattctatat tcactaacga attctctgtt attattccgc
      781 catcctatta tgatgcgact tctgtagtaa acgccaataa tggtgtgaga gaaaaactat
      841 ttgaaagtga tagtaaagtt aaagatatcg tcgatgatat gtctaatcat gatgtggaga
      901 gcgaaaaaga tatatttgtt atcggaggaa tgatagaaaa actggattct ctggtagacg
      961 aatctttcaa tgaaaatact gataacatac aaacagtaaa agatttgtta acgcaattaa
     1021 ctgatggcat ggagctcttc gcgctgcggg acatagtggc at
//
LOCUS       DAPGRN010000307         1046 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPGRN010000307 DAPGRN010000000
VERSION     DAPGRN010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.313"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(243..884)
                     /locus_tag="RHW33_004872"
     CDS             complement(243..884)
                     /locus_tag="RHW33_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HDV1648275.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VANSDYIRAMAGEVEPRQEPSLLSPLINIIRGTQRLAEKSVPHGILTGKGNYF"
BASE COUNT          274 a          244 c          229 g          299 t
ORIGIN      
        1 aaacaaaaaa cgtagatagt aaggatagtt tacggcttga agaaaaaaag aaaattgaac
       61 atgcccaggc gcttttcaat caaataagtc agtctgttaa agttgaattc aaaacgcagt
      121 ttgctaatga tgatatttat cagttaatca agagcgcatt accataattc cagtttaaaa
      181 ggaggtgaaa acctcctttt tatgacatcc aacacgacct gaaagaagtg gcgctcaggt
      241 cgttaaaaat agtttccctt tccagttaag attccatgtg gaacgctttt ttccgcaagt
      301 ctctgtgtgc cgcgaattat gttaatcagt gggcttagta acgatggttc ctggcgtggc
      361 tcaacttctc cagccattgc cctgatgtag tcggagtttg caacgttgtt gtattccgtt
      421 gcgaagcagc ataacaacgt cagaacgtgc tctgtcgtta tttcgctcca gttgatgttg
      481 aaaaattcat cgcctttttt gtcatgttcg gaatcgaaga tgctttggtg gaggatgtat
      541 ttgccggatt ccttgcgcgg taacttgatc gctttctggc gttccaattc cttgtaaatc
      601 tgcattgctt caatcagtac cggtctaccg ttcatgaagg gatcacgcaa ccttacacgc
      661 tggccaactc gaccggtaat aaagctgttt tcctcttcca ccagcacgat aaaacccttt
      721 tcctcttttt ctcgcaattc gcgcagcagc tggagttcca tatcgcggcg gcgttcaggg
      781 tagctggtcc gctcagccat tatcagctca ttgttgatcc atgcagcagt cattgacgcc
      841 ggtttgccga cgctcattga aacaacgcat attttcttat ccatagcgcc ccctacaaaa
      901 aagaaaagcc accagcggcg gcttagcaat acaacagaag gtagcgcccg gtactcagac
      961 tgtgccgtcc atggaatatt tgaaaaggga tccatccgta ccgggcgtgt gatgattctg
     1021 actcaagtca cttgtcagtt gtcaat
//
LOCUS       DAPGRN010000308         1040 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPGRN010000308 DAPGRN010000000
VERSION     DAPGRN010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.314"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            142..444
                     /locus_tag="RHW33_004873"
     CDS             142..444
                     /locus_tag="RHW33_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV1648276.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHIVNGANWFDRTVSA
                     DACGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            491..913
                     /locus_tag="RHW33_004874"
     CDS             491..913
                     /locus_tag="RHW33_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HDV1648277.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKRVSRQVTVPAVMLEKVVALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
BASE COUNT          241 a          240 c          325 g          234 t
ORIGIN      
        1 acaacggagg aatacatcat gcaatatgcg aaacctgtca ctctgaatgt tgaagagtgc
       61 gaccgtctgt tttttctgcc ttacctgttt ggccaggatt ttctgtatgt cgaagcgtct
      121 gtgtacgcgc tggcgaaaaa aatgatgccg gaatatcagg gaggcttctg gcacttcatc
      181 cgcctgccgg acggtggcgg ctacatgatg ccggatggcg accgtttcca catagtgaac
      241 ggtgcaaact ggtttgaccg taccgtgagt gctgatgcct gcggcatcat tctgacttcc
      301 cttgtgatta accgccagtt gtggctgtac cacgacagcg gtgatgcagg actgacccag
      361 ctttaccgga tgcgcgatgc gcagttgtgg cgtcacatcg aatttcaccc tgaatgcaac
      421 gcgatttacg cggctctgga ctgattaacg gacggggcgg caatgccgcc cctgtaaagg
      481 agacgagaaa atgtactgta ctgttaaaga aattatccgc gaagtgctgg atacagacgt
      541 gccggacagt gaatgcgttt ttgccgtggt gctgacccgt ggggatgtgc gtcacatagc
      601 ccaggactgg aacctgtcag acgatgagct ggaaaccgtc atgcagcggc tggacgatgc
      661 ctttgagcat ggtgcggatg tcagcgttgt tcacgacgtt gttcgtgaac tgatggaaga
      721 aaagcgcgtc agccgtcagg tgacagtccc ggcggtgatg ctggaaaaag tggtggcgct
      781 ggcaggcagt gaaatgaagc gcctgtatgc cgtcgggagt gagaacgggg gcgacggtga
      841 cgcattcgtc agggaagaac gcgaagcgat ggacgttgtg ttacaggcgc tggacgggga
      901 gcacatgtca tgaatatcag cacagaaacc cgcgaaattc tgcgcaatta caaagccgtg
      961 attaatgcgc ggcgtcgtga gatggggcag aaaccgctca ccactgcgca gattgttgat
     1021 gaaatctgcg attttgtggt
//
LOCUS       DAPGRN010000309         1036 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPGRN010000309 DAPGRN010000000
VERSION     DAPGRN010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.315"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            96..353
                     /locus_tag="RHW33_004875"
     CDS             96..353
                     /locus_tag="RHW33_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648278.1"
                     /translation="MDQEMTFSLSYEQLTRFAERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPEDDR"
     gene            353..943
                     /locus_tag="RHW33_004876"
     CDS             353..943
                     /locus_tag="RHW33_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648279.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
BASE COUNT          240 a          280 c          320 g          196 t
ORIGIN      
        1 aaaaccatat ctgatattat cagaaatcag gagcacacaa atcattcagg atatgattat
       61 tacttgtcag aatattatca gatggagcgt gcaggatgga tcaggaaatg acgttttcac
      121 tcagctatga acaactgacc cggtttgcgg aacgacgcat ccgggaatgc aatctggaca
      181 gccagggcgc gatttatctc tgcgagtcag ccaaagccgg agccgtgctg atcttctggc
      241 atgaactggc cataaatggc tatgcctcaa tgaatgccat aaagagacag gagctcattg
      301 acgccgactt tcagcgcctc agaaacctca tctggccgga ggatgacagg taatgaccca
      361 gagccgccga ccgtcgccac tgcagcggag agtgctgatt gtgctggctg cccttgatga
      421 aaaacggcca ggacccgtgc tgacgcggga catagaacgg gtgctggagc gaagcgggga
      481 agccccggtc tacgggccaa acctgcgcgc ctcctgccgt cgtctggagg atgccggctg
      541 gctgcgtacc ctgcgcgctc cgaacctgca gctggcagta gagctgacgg acgccggccg
      601 ggccgtggca cagcctctgc tgctggcaga acaggaccgc ctccgggcag aacagcgtgc
      661 ggcagaagtc gtcgtactgc cgctggtccc ggcagcaggt ctgcccgcag atggcacttc
      721 tgccactgat ctggcggtgc aactgaacgg cataacgtat caggcctgcc gtggggattt
      781 tgtggtgcgt ctagacggca gcacctgtct gcagttatgg aataaagaag gcagggtggt
      841 acgccgggag ggcgatccgc tggaagtggc gcagtggctg caggcctgtc acgatgcagg
      901 aatggaggtt cgcgtacaga taaatgaaag tgccgctccg taagaacacg cctgtgcacg
      961 gtttacacat catcccatgg tggcagccgg ttaactttcc tgcgccacca gtggaaagag
     1021 gccatgcaca gtccgg
//
LOCUS       DAPGRN010000310          998 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPGRN010000310 DAPGRN010000000
VERSION     DAPGRN010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.316"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..130)
                     /locus_tag="RHW33_004877"
     CDS             complement(<1..130)
                     /locus_tag="RHW33_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HDV1648280.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNP"
     gene            complement(265..780)
                     /gene="traV"
                     /locus_tag="RHW33_004878"
     CDS             complement(265..780)
                     /gene="traV"
                     /locus_tag="RHW33_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HDV1648281.1"
                     /translation="MKKISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(777..>998)
                     /locus_tag="RHW33_004879"
     CDS             complement(777..>998)
                     /locus_tag="RHW33_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HDV1648282.1"
                     /translation="RIVLPALKEFGSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVV
                     FGQPCAGKTNLTAELISAVVENNKKGEGQ"
BASE COUNT          238 a          261 c          225 g          274 t
ORIGIN      
        1 taggatttcc gcatgcttca cagagataaa caggaatacc atgagcattt attttctggc
       61 gtatccggtt tattcctgtc atggtcagtt gttctgtcac tgaatatgct tcatcggctt
      121 catcactcac ggaatattcc tcccgacatg ttctgacacg ctaccgccca tcccttttca
      181 cgcacagggc actcgtttta caggcagatt ttgaatacag aaagcccgcc ggcattgccg
      241 gctgatatac aacttcaggg ccagttaatt aatacgtggt tttccccacg cagacggttt
      301 aataacaaaa aacacgctgg atggctgatg ataaacatcc tggttatcaa tataaggcgc
      361 tatccataat gctgccgtct gctcacccgt tcttagcgga cgaggaggtg ttaccggcgt
      421 aactgaactg accggaacaa ccgttttcac ttcccgggca gcggtaaaca gcgggcgtgg
      481 tgccagcagt ttctgctccg gatgtgctgt caccgcaggt ctgctgccgg acggagtggt
      541 cgcggtgacg gtctgtaccg gcattgtccg gaagttccct tcagccaggc gcggcagtga
      601 tgccgcaacc ggctttgctt ctgaggactg ctccagtttt ttggccttct cattggcctg
      661 ctccatcgtc atacaggtat ctgatgtggt ggcgttacac tcaaattccg tattagttcc
      721 ggcacagcca gaaagtaata acgtccccag cagaggaata aataaagaaa tctttttcat
      781 tgcccttctc cttttttatt attctccaca acagcagaaa ttaactctgc tgtcagattt
      841 gttttccctg cacagggttg tccgaagact actgtatttc ccggagaaga aaataagctc
      901 atcgacatac ccttcttcat cacaccgaca gctgccatat taaaattgtg cggcaatgat
      961 tcagaaccga attccttcag tgcaggaagt acgattct
//
LOCUS       DAPGRN010000311          995 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPGRN010000311 DAPGRN010000000
VERSION     DAPGRN010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.317"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(222..644)
                     /locus_tag="RHW33_004880"
     CDS             complement(222..644)
                     /locus_tag="RHW33_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648283.1"
                     /translation="MIPSQISFNTLPVNATYASEPTVDTQKFSDILYSAGCSLKDLAY
                     LSRCLASIHPTIAKNIYETENLSDQKLLHIDCRSTNEIKINIFFGQQREGLIEISSDT
                     VIFSILSSLIDSAISKFQHQNPVNGSVNRELLYEDYTD"
     gene            complement(725..>995)
                     /locus_tag="RHW33_004881"
     CDS             complement(725..>995)
                     /locus_tag="RHW33_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648284.1"
                     /translation="LMKCERSIHDHNRYVTGKDTLLLDYPPSGNQIYFHIFPDESATL
                     VLYFSNNDIECFVFERDIPSQYRFFKMFTNLALVIDQLTDANQIL"
BASE COUNT          316 a          157 c          208 g          314 t
ORIGIN      
        1 atttaagtcg aatttcactg caattcatct ctgcttaatg taaaaataat gttgaaaata
       61 acaaatggtt aatgattcat gcgaattatg accttagtta gctcttttga tttttcgtaa
      121 aaaagagagg tacatgaagg tgctaccagt atgagtaacg ttaatcaaag gttttattac
      181 gtctggcaat aacgcaggcc atttttcgcg acagggtgga tttaatcagt ataatcctca
      241 tacaatagtt cacgattaac tgaaccgtta accgggtttt ggtgttgaaa tttagaaatg
      301 gcggagtcaa tcaatgaaga cagtatcgaa aaaataactg tgtcagaact aatttcaatt
      361 aatccttctc gttgctgacc aaaaaagata tttatcttga tttcatttgt actacggcaa
      421 tcaatatgca gtaatttttg atcgctgaga ttttctgttt cataaatgtt tttggcgata
      481 gtcggatgta ttgatgccag acatctgctc agataagcca gatcctttag ggaacacccc
      541 gctgagtaga ggatgtcaga gaatttttgt gtgtcaactg ttggctctga agcgtatgtt
      601 gcattgacag gaagagtatt gaaagagatc tgagatggta tcattattta aatctttccg
      661 tgagtaataa aaattaatcc ccgggcattg tttagcaata tttgccacag gggatatata
      721 aaaattaaag tatttgattg gcatcggtta actgatcaat aaccagtgcc agattggtaa
      781 acattttgaa aaagcgatac tggctcggaa tatctctttc aaagacgaaa cattcaatgt
      841 cattgtttga aaaataaagg actagtgttg ctgattcgtc aggaaaaata tggaaatata
      901 tctggttacc agaaggagga tagtccagaa gaagggtatc tttgcccgtc acatacctgt
      961 tgtggtcgtg gatgctgcgc tcacacttca tcaga
//
LOCUS       DAPGRN010000312          993 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPGRN010000312 DAPGRN010000000
VERSION     DAPGRN010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.318"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..457
                     /locus_tag="RHW33_004882"
     CDS             <1..457
                     /locus_tag="RHW33_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HDV1648285.1"
                     /translation="NNKDREKKYRDGDKINGPQAHSLDSTNERRFADNRTMFTQHIEK
                     QRNVHTLNQNDINNSVNSVNVPENELTYQFQRWGQNHTVRILESSEGIRLKPSDALVS
                     DRLHEAQNNDVTAQRWVLTEQDERQGQRHQPHEEQENEGKFENDHKDES"
     gene            457..>993
                     /locus_tag="RHW33_004883"
     CDS             457..>993
                     /locus_tag="RHW33_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HDV1648286.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLREIFPLISSELAGMALMPEMERLIGEWLSLSSSPLNLKHPELKYNRL
                     CVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYWPIHFVIGFS
                     KTCYRTIVDIEVGDVLLIS"
BASE COUNT          349 a          165 c          219 g          260 t
ORIGIN      
        1 taataataaa gatcgtgaga aaaaataccg cgatggagac aaaataaatg gaccgcaggc
       61 tcattcatta gacagtacta acgaaagaag gtttgctgat aacagaacca tgtttactca
      121 acatatcgaa aagcaaagga atgttcatac gctcaatcag aatgatataa ataattccgt
      181 taatagtgtc aatgtgccag aaaatgaatt gacatatcag tttcagcgtt ggggacagaa
      241 tcatacagtc cggattctgg aaagtagtga agggatacga ctaaaaccat cagacgcgct
      301 tgtctccgac agactccatg aagcccaaaa caatgatgtt acagcacaac gttgggtatt
      361 gactgagcaa gatgaacgac aaggacaacg acatcagcct catgaggagc aagaaaatga
      421 aggcaaattc gaaaacgatc ataaggatga aagttaatgt ttggtctgag gaaagtaaat
      481 cgaaacactc atacgtttga aaccactttt caaaattgga aagaaaatgg cgaagatgtt
      541 gcattattaa tgcccgaatt tagtgctaaa tggctaccaa tagcagaaga gagtggcagc
      601 tggtctggat gggtattgct tcgggaaata tttcccttaa tttcttctga gctcgctggg
      661 atggctttaa tgccagaaat ggaaaggtta ataggggaat ggctcagttt atccagttcc
      721 ccgttaaatc taaagcatcc tgaactaaaa tataatcgtt tgtgcgtagg taaagtattt
      781 gatggagtat tgagcccagc tcaaccacta ataagaatat ggacagggga attgaatctt
      841 tggttagata aagtcacagt ctgccaatac ggaaacgctc cagcgttaga caaaaaatca
      901 ttatattggc ccattcattt tgtgattgga tttagtaaaa catgttatag aactattgtt
      961 gatattgaag tcggtgatgt tttattaatc tca
//
LOCUS       DAPGRN010000313          974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPGRN010000313 DAPGRN010000000
VERSION     DAPGRN010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.319"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(609..784)
                     /locus_tag="RHW33_004884"
                     /pseudo
     CDS             complement(609..784)
                     /locus_tag="RHW33_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          303 a          196 c          197 g          278 t
ORIGIN      
        1 tattaccagt aatttttacc cattcagtta ttaagctctc aggtataagt gacttcaaaa
       61 tacatagcta cattattaaa acaagtgaat atccggagga gtttttttcc aatgcagtat
      121 ggggtaaaaa aaatataaaa ccgggggaat attactctgt gcaggctgtt tctatgttta
      181 cgacaaacca gtttattctc ctttgtccca aggatattat aaggttttat cgggaaagct
      241 ggaagttcga cctgcttaat gtagacttcg atacaaatac caggaagaaa ctccaggaag
      301 aagcagcata ttgcgtacca atttctgcaa tatctgtaaa acgatgggat atgcctcttc
      361 agggctcgaa accctcaaat tagaaggccg ctcgtgcgac tgtattagat agtaatgcgg
      421 tatggaaaaa tagtgatggc aagatgaact tgacttggct aatcaaatac cggcgtagtg
      481 attaggaaac agggctcgga taacattatt gcgcggttca ttgaacctac tgatgacgcc
      541 ggatatatag tcgtaaaccg tttcgaacca gtacttatat caccaggaga gtccatatat
      601 gaccaccgtt tacagtaaaa acggtatctg ttttctgatc cctccagcgt caaactaccg
      661 ttgtaatggt gaggtctggt gctattgtaa taattcagaa tataatctct gatttatttc
      721 cgaacctcgc ccttgcctgc gtaaccatcc gtcgccccca tcctgttttc agactgcgga
      781 aaaaggcaag tttttatccc cccacagaga ttcaatcata tatcgcgtgt tttggaagtg
      841 acagttctta ttcctgagaa tacagcatgt gcaacacact cggcatccat cgataccact
      901 tttatctcta ataggcagga taaaaaacaa gactgtgaat taaagtaatc ggcaaatgaa
      961 tgagttagaa gata
//
LOCUS       DAPGRN010000314          954 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPGRN010000314 DAPGRN010000000
VERSION     DAPGRN010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.320"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..304
                     /locus_tag="RHW33_004885"
     CDS             26..304
                     /locus_tag="RHW33_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDV1648287.1"
                     /translation="MQINQMHIPMLKKRGIIQDERDLLDNPCLNIKIGTEILYNHFSR
                     CGVTWQCLGTYNAGFAMDNKKKRLQYAKKIYIVYTRLNELDNRKALAK"
     gene            complement(322..954)
                     /locus_tag="RHW33_004886"
     CDS             complement(322..954)
                     /locus_tag="RHW33_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV1648288.1"
                     /translation="MGKIKIVVSDQQPFMIDGIIGFLGHYPDLYEVVGGYKDLKKAIA
                     ECNKSAAQIFILGEFAGGMMGSELVKWVKSHKIDAHIITFVAKMPYIDSIKLLEAGAK
                     GCVWKTSHPAKLNRAIDSISNGYTYFDSVHMDCEKISSRYSSDNQLTNRESEILQLIA
                     DGKTNKEIANFLQLSRKTVETHRLNIMKKLDVHSGIELIKTALRMGVCTI"
BASE COUNT          306 a          184 c          156 g          308 t
ORIGIN      
        1 tataaggagt ttagattatg ggttaatgca gataaaccag atgcacattc ccatgttaaa
       61 aaaacgtgga ataatacaag atgaacgaga tttgttagat aacccctgtc tgaacattaa
      121 aataggaacc gagattttat ataaccattt ttcacgttgt ggagtaacgt ggcaatgcct
      181 ggggacttat aatgcgggtt ttgcaatgga taacaaaaaa aagagactgc aatatgccaa
      241 aaaaatatac attgtatata ccaggcttaa tgaactagac aatcgtaagg ctttagcaaa
      301 atgagcctaa agcctctttt tttatatagt gcaaacaccc atacgtaaag ctgttttgat
      361 caactcgata ccactgtgaa catctaattt cttcatgata ttgagtctat gagtttcgac
      421 tgttttcctg cttaactgca agaaattagc gatttctttg tttgttttac catcagctat
      481 gagttgtaat atttctgact cacgatttgt aagttgatta tcagaggaat acctggaaga
      541 tattttttca caatccatat gtacactatc aaaataagtg tagccattac taatcgaatc
      601 aatagcgcga tttagtttcg ccgggtgact ggttttccat acgcatcctt tcgcacctgc
      661 ttcaagcaat tttattgaat caatatacgg catctttgct acaaatgtaa taatatgagc
      721 atctatttta tgcgatttaa cccattttac gagttcggac cccatcattc cacctgcaaa
      781 ttctcctaaa ataaatattt gggctgcaga tttgttacac tccgctatag ctttcttcag
      841 atctttatag cccccaacaa cctcatataa atcaggataa tgtccgagga atccaattat
      901 cccatcaatc ataaacggct gctgatctga aactacaatt ttaatttttc ccat
//
LOCUS       DAPGRN010000315          925 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPGRN010000315 DAPGRN010000000
VERSION     DAPGRN010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.321"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..812
                     /gene="gadX"
                     /locus_tag="RHW33_004887"
     CDS             <1..812
                     /gene="gadX"
                     /locus_tag="RHW33_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV1648289.1"
                     /translation="GNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVE
                     NFVFVSRDTLSLFLPMLKEEALNLHAHKKVSSLLVHHCSRDIPVFQEVAQLSQNKNLR
                     YAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIA
                     SELLMSPSLLKKKLREEETSYSQLLTECRMKRALQLIVIHGFSIKRVAVSCGYHSVSY
                     FIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          266 a          174 c          211 g          274 t
ORIGIN      
        1 atgggaattg tctaattgcg tatgcaagac ataaatatat tcttaccatg gttaatggtg
       61 aatatcgcta ttttaatggc ggtgacctgg tttttgcgga tgcaagccaa attcgagtag
      121 ataagtgtgt tgaaaatttt gtattcgtgt caagggacac gctttcatta tttctcccga
      181 tgctcaagga ggaggcatta aatcttcatg cacataaaaa agtttcttca ttactcgttc
      241 atcactgtag cagagatatt cctgtttttc aggaagttgc gcaactatcg cagaataaga
      301 atcttcgcta tgcagaaatg ctacgtaaaa gagcattaat ctttgcgttg ttatctgttt
      361 ttcttgagga tgagcacttt ataccgctgc ttctgaacgt tttacaaccg aacatgcgaa
      421 cacgagtttg tacggttatc aataataata tcgcccatga gtggacacta gcccgaatcg
      481 ccagcgagct gttgatgagt ccaagtctgt taaagaaaaa attgcgcgaa gaagagacat
      541 catattcaca attgcttact gagtgtagaa tgaagcgtgc tttgcaactt attgttatac
      601 atggtttttc aattaagcga gttgcagtgt cctgtggata tcacagcgtg tcgtatttca
      661 tttacgtctt tcgaaattat tatgggatga cgcccacaga gtatcaggag cgatcggcgc
      721 agggattgcc gaaccgtgac tcggcggcaa gtattgttgc gcaaggaaat ttttacggca
      781 ctgaccgttc tgcggaagga ataagattat agaattttac tcagacataa aaaaaacccg
      841 gcatagggga ccgggaagag gatagtctgc cgtctccaga ctaataaacc gttataacac
      901 tccctgttgg cacgggaaac tttgt
//
LOCUS       DAPGRN010000316          913 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPGRN010000316 DAPGRN010000000
VERSION     DAPGRN010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.322"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(86..757)
                     /gene="queE"
                     /locus_tag="RHW33_004888"
     CDS             complement(86..757)
                     /gene="queE"
                     /locus_tag="RHW33_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV1648290.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          246 a          220 c          202 g          245 t
ORIGIN      
        1 ggaggttttt cagagacgac aaagaatata aatgagtaga agtctttaca gattccaccc
       61 atttataacg cttataaatg tttaatcagg caatatttaa atatttatgg gtttgcatcg
      121 acaaacgcca attacgcgca atgcaggttt caatgcacaa acgtgtggca tcatcctttt
      181 ggctaatcgg ctgcagtgca atgactcgcg gtttatcatc ggtcagcgtc gccaaaagtt
      241 catccagtgc ttcaatatca cgtacgcgtc ccactggatg cttgatttcg ttggctcgct
      301 ccagtgcctg tgacaacact tcatagccgc cgcgcatgtt cagctttggc gatacggtaa
      361 cccaggtatt cggtgtacag cgtacttcat gagtaccact ggtttcgatc tggcagctaa
      421 aaccgttctt ttcgagcagg tcagtcagtg gcagcaaatc atgaatgcaa ggctcaccac
      481 ccgtaatcac cacatgccgc gcggtatatc cctggcgacc aatgacagcc agcaagtctt
      541 cactgctcgc cgccccccac ttatcactct ctttggtctt cgccagaatg ctgaaaaggg
      601 agacttcccg atcctcaagc ttttcccagg tgtgtttggt gtcgcaccag gcacagccaa
      661 ccgggcatcc ctgtaaacga ataaaaatgg cggggacgcc ggtaaagtaa ccctcacctt
      721 gcagggtctg gaacatctcg ttaatcgggt actgcatagc attctctgtg aagtggataa
      781 ttgttaatta ttgcagatcc tgccacaaca atcatgtctt attaacattc tgttacaggc
      841 aggtttatgg caaaaagtgg attagatcat aaagaataaa atgaagcaga gtattatgtt
      901 ttaaggaatt aat
//
LOCUS       DAPGRN010000317          898 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPGRN010000317 DAPGRN010000000
VERSION     DAPGRN010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.323"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..673
                     /locus_tag="RHW33_004889"
     CDS             32..673
                     /locus_tag="RHW33_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648291.1"
                     /translation="MTALIDYENCGSLKNISLEQYGELIIFVGPQQNVVVLPADSFPE
                     GARITIRQVSGVSRNNVDFHLVLELGRISCCAAGKDKTYHIISSDKGYDGVIRTLKEA
                     GITCIRRDVPESIAAAGESGADKSNCLKQPSVPESDIVSRWAGKLQQIAGSGAGNMPV
                     SVETFNNYLKSHMRDEWNKTRAVAIRNELVRRGVTEVREKKIIWKKAKKENKA"
BASE COUNT          270 a          167 c          245 g          216 t
ORIGIN      
        1 agaaaataat acgagtacag ataaaaacac cctgacagcc cttattgatt atgaaaactg
       61 tggcagcctg aaaaatattt cccttgagca gtatggggaa ctgattattt ttgtcgggcc
      121 acagcagaac gtcgttgttc ttcctgcaga ctcttttcct gaaggtgcga gaatcactat
      181 caggcaggtg agtggtgtgt ccaggaataa tgtcgatttt catcttgttc tggagctggg
      241 gcggataagc tgttgtgcag cagggaaaga taaaacatac cacatcattt catcggataa
      301 gggatatgac ggtgttatca ggacgctgaa agaagccggt attacctgca ttcgtcgtga
      361 tgtaccggaa agtattgctg ctgccggtga gtctggtgca gacaaaagta actgcctgaa
      421 acaaccttct gttcctgaaa gtgatatcgt cagccgttgg gcaggaaagc tgcagcagat
      481 agccggcagc ggagccggta atatgcctgt gtccgtcgaa acatttaata attatttaaa
      541 gtcacacatg cgtgacgaat ggaacaaaac ccgggcagtg gcgataagga atgaactggt
      601 tcgtcgggga gtcacagaag tcagggagaa aaagataatc tggaaaaagg cgaaaaagga
      661 gaataaagca tgaggtatcg cagctgtgct cagataaaaa cagcccccgt tgcggttgcg
      721 gagcgaaggg ggctgaaaag gggaattaag gatgattgtt attatggtga accagaaggt
      781 tatccctgtc tgtaagctga atcggactaa acgacataat gcctgtaaca tcactgaaac
      841 atcagcggta tatgtggtgt gtatgaatat cccggcagca ttaattcttc tgtgagga
//
LOCUS       DAPGRN010000318          897 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPGRN010000318 DAPGRN010000000
VERSION     DAPGRN010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.324"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..351)
                     /locus_tag="RHW33_004890"
     CDS             complement(<1..351)
                     /locus_tag="RHW33_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648292.1"
                     /translation="MKIGTVAGTNDSITTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIETNHSEKYSMLLGMRAHILRR
                     DNNYQFIVTINSKNY"
     gene            complement(613..>897)
                     /locus_tag="RHW33_004891"
     CDS             complement(613..>897)
                     /locus_tag="RHW33_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV1648293.1"
                     /translation="RINTMEPTPVYADYLNNTTDFRPARERIFICGETLKCEDPSKHI
                     RQKYGHHGLGVNQNQQFDNGVMGLNSRMAVFDENKKIIGSSSGLSCFQPR"
BASE COUNT          273 a          180 c          137 g          307 t
ORIGIN      
        1 gtaattttta ctattgatcg tcacaataaa ctgataatta ttatccctac gtagaatatg
       61 agcacgcatg cccaatagca tactatattt ttctgaatga ttcgtttcta tggtaaatga
      121 tattttgtca tcttgaatag attgttggaa ccgttctatt tgccaatcat ctattttttc
      181 cacaacctca ccgagcattg tcctggcaat acttgatata tcatcaccta taccatttgc
      241 aatataccca aacagttctt tagtgaagtt ggtaacatgc tcttgcacca tatcatttgt
      301 tgcgattgtc gttatactgt cgttagtacc tgccacagtt ccaattttca tcttttacct
      361 catataattc accgcgagga atacatagtt atcatttaat cttatgacat acacctcatg
      421 ttcgatggaa aaataattat attttgcaca aatcattcag ccttaaagca taatatgaaa
      481 tttcttatct catagtagag aaagtcccta aagattgtag aggcgtcatc agtacatacc
      541 tctacaatcg attaagaaaa cctatgaaat actggcagga ttaaaaaaac ataccacagc
      601 actcatggag ttttaacgag gttgaaaaca actaagccct gaggaagagc ctattatttt
      661 tttattttca tcaaatactg ccatcctgga atttagcccc ataacaccat tatcaaattg
      721 ttgattctga tttaccccca acccatgatg gccatatttt tgtctaatgt gtttgctggg
      781 gtcttcacat ttgagtgtct ccccacatat gaagatacgt tcacgtgctg gtctgaaatc
      841 agtagtgtta tttaaataat cagcatatac aggtgtgggt tccatagtat tgattct
//
LOCUS       DAPGRN010000319          892 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPGRN010000319 DAPGRN010000000
VERSION     DAPGRN010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.325"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..496)
                     /locus_tag="RHW33_004892"
     CDS             complement(<1..496)
                     /locus_tag="RHW33_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648294.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEKCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAYFNKTSIT
                     SDCWQ"
     gene            complement(752..>892)
                     /locus_tag="RHW33_004893"
     CDS             complement(752..>892)
                     /locus_tag="RHW33_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV1648295.1"
                     /translation="YLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECNR
                     FP"
BASE COUNT          264 a          164 c          170 g          294 t
ORIGIN      
        1 tttgccaaca atctgaagta atgctggttt tattaaagta cgcagggggt gttaagatgg
       61 cttcttttcg ggtgaatttt gcacaatact tagaaatcgt ctccttaata cattccagct
      121 cttttttatt gacggctgag caagtacatt tttcgccaaa aggtgcaaaa gtgcctacat
      181 ctaatgtttg tatgatataa tcgtcatgtt ctcctgtaga aatatctatg atgaatggac
      241 tcatattttc cggttggatt cggatcatga cagaaggaac atcgccttga tagaaattca
      301 atgcagtaat tgcagataat actctccgca gttcattact tatactgtaa attttattaa
      361 cgtcattggt ggagacaatt tctgcaaact ttctttttac cgtccaataa tcaatatttc
      421 tattgtgatt gttaaccact gcgtgcattg gcgggattga agggtgaaaa atatgtgcaa
      481 ttgggaagcc tttcattgat gcctgccttg tcgtcatgac aaacaataat agatgcttat
      541 tttcctcgca gggttctttc gcactatcaa aaaacgctca tttacccggc gttacagtaa
      601 acgaagcaac attctgtggc ctacgccgat caaaagctct gtgaaaattt ctcttgttgt
      661 ctttgtctga ccttatcgcg gcgatcagca attattcgtt ttgtgatcct caacacaaaa
      721 tgctattcgt cgcctatgag gggagaagaa tttatggaaa acgattgcat tcacaaaatg
      781 acgtcctgtc agaagagaaa aagttgacgt taatcaattg ttcgagatga tcaatacctg
      841 tattcgaaat tttaaaatat tgataaaata aatgtttgtt ctccaacaaa ta
//
LOCUS       DAPGRN010000320          884 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPGRN010000320 DAPGRN010000000
VERSION     DAPGRN010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.326"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            15..776
                     /gene="ydeO"
                     /locus_tag="RHW33_004894"
     CDS             15..776
                     /gene="ydeO"
                     /locus_tag="RHW33_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV1648296.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFATCKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMNALAI"
BASE COUNT          273 a          169 c          175 g          267 t
ORIGIN      
        1 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
       61 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
      121 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agaaatcgga tttaatgaaa
      181 ataccattaa aaaatatcta caatgcacta acatccagac agtgacgatg cctgttcctg
      241 cgaagttttt acgtgcttct aatgtaccga ctggattgct taatgaaatg attgcttatc
      301 tcaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
      361 ctatttttgc cacatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
      421 cggggaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
      481 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
      541 cgacattctc acagattctt ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
      601 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
      661 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
      721 gtcaaaggca cacgggtatg aatacgggca acacgatgaa tgctttagct atttgattat
      781 ttgctaacga gtagtcaacc acacacgcta cgtaagaatt aaatggggca gccattcact
      841 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taag
//
LOCUS       DAPGRN010000321          883 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPGRN010000321 DAPGRN010000000
VERSION     DAPGRN010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.327"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..849)
                     /locus_tag="RHW33_004895"
     CDS             complement(<1..849)
                     /locus_tag="RHW33_004895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDV1648297.1"
                     /translation="MMNISDGKFMDLNDEKRAFLELNVFKGKILKPLKGRGGLIYIVE
                     RNCIPKFVAYKTTQEFERLDISSIRVESIVNIEREAGNWFRYSNHPLLITPFSVTLVN
                     NFPIICMPCCNGDLSVFTRSKQKFTSVIFLSLQIIKGLIEANKAGLKHHQDLKPENIL
                     YIDLSEKFINYPPIDVDPFLRYSVRIADFGVANAYLNGHPGGTNVYKAPEQYDIELFS
                     GCFEPDIFAVGIIIAELFQGYHPAALDKDKDKRIRNWKGKKLKRWALSGIRNYKAAES
                     DNEVSLI"
BASE COUNT          272 a          181 c          126 g          304 t
ORIGIN      
        1 tattaaagaa acttcattgt cactctcggc agctttatag tttcttatcc cagataatgc
       61 ccaacgctta agttttttac ccttccaatt cctaattcgc ttatctttat ctttatctaa
      121 ggcagctgga tggtagcctt ggaaaagttc tgcaattata attcctacag caaaaatatc
      181 tggctcgaaa caccctgaaa acagttcaat atcatactgc tccggtgcct tatatacatt
      241 tgtcccgcca gggtgaccat ttagatatgc atttgccaca ccaaaatctg caattctaac
      301 tgagtacctc aaaaagggat caacatctat aggtgggtag tttataaatt tttcacttag
      361 atcaatatag agtatatttt ccggctttaa gtcttgatgg tgttttagac ctgctttatt
      421 cgcctctatc aatcctttaa taatttgcaa tgataaaaaa ataacactgg taaatttttg
      481 cttagaccga gtgaagacac taagatctcc attacaacag ggcatgcaaa ttatagggaa
      541 attattgacc aatgttactg aaaatggtgt tattagcaaa ggatgattgg aatatctaaa
      601 ccaattccct gcttcccttt cgatatttac aatggattcg actctaatac tcgaaatatc
      661 cagcctctca aattcctgag tggttttata agcaacgaat tttggaatac aatttctctc
      721 aactatatat atcagaccac ctcgaccttt aagtggcttt agtattttcc ctttaaaaac
      781 atttagttct aagaacgctc tcttttcatc atttaaatcc ataaatttcc catctgaaat
      841 gttcatcaca ctcataattc cttatctgat tattaatggt tta
//
LOCUS       DAPGRN010000322          872 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPGRN010000322 DAPGRN010000000
VERSION     DAPGRN010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.328"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..355)
                     /gene="traA"
                     /locus_tag="RHW33_004896"
     CDS             complement(<1..355)
                     /gene="traA"
                     /locus_tag="RHW33_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HDV1648298.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLVRAVLPAAVLMMF
                     FPQLAMASGSQDLMASGNSTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVG"
     gene            complement(388..783)
                     /gene="traY"
                     /locus_tag="RHW33_004897"
     CDS             complement(388..783)
                     /gene="traY"
                     /locus_tag="RHW33_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HDV1648299.1"
                     /translation="MKRFGTRSATGKMVKLKLPLDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
BASE COUNT          265 a          209 c          156 g          242 t
ORIGIN      
        1 ggccaacgac ggccataccc acagcaataa atacagagat gatggcaaaa ccggccagga
       61 acttgacgtt tttggtcatc atgtacatga cagcaccgac cagaacttca gccagaacaa
      121 cccatttaac aacactggag tccttaccga aggtcgcctt aaccgtggag ttaccacttg
      181 ccatcaggtc ctgactaccg gaagccatcg ccagttgcgg gaagaacatc atcagaacag
      241 cagccgggag cacagcgcgt accaggcgaa gcatattcag acgagtgaat ttagaaaaaa
      301 acgacttctt tttgacgggc gcagaagcac cctgaacact taaaacagca ttcataagtt
      361 acctcaattt cggataaatg aataaaacta gagtgtatta aatgttatat cttcctcttt
      421 atctgcctcc ctgaatatct ccgagttata aaaatcagga aacttgatca aatgatcaat
      481 aactctgtct gcagcctcat ctgttttaca ccatccacta cgatttttgg ctgcaataag
      541 tagctggtta gtttcatcat ccagacgcat tgtcagatac ttaaccagcg acttaccata
      601 gatattccct gccctgttaa actttggata gcggtgaaga tgatctttaa gtcttattac
      661 tgcctcaaac gatcgacttc ttccgcttct gttacttgcc tcaattaata gactttccac
      721 atctaaaggt aattttagtt ttaccatctt acctgttgca gaacgtgtac caaatctttt
      781 caataacacc tcccgctgtt tatcttctgg ttaccactta tgtttgcaga ataatcagaa
      841 aaggtaaata gacggaaagt ctatatttta tt
//
LOCUS       DAPGRN010000323          864 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPGRN010000323 DAPGRN010000000
VERSION     DAPGRN010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.329"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(172..735)
                     /gene="ipbA"
                     /locus_tag="RHW33_004898"
                     /gene_synonym="fimX"
     CDS             complement(172..735)
                     /gene="ipbA"
                     /locus_tag="RHW33_004898"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV1648300.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGRRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          260 a          166 c          173 g          265 t
ORIGIN      
        1 gttaatcact ggtatgacct ttgtgtttaa tcatttcaaa tcatggggtt attttacttt
       61 agcgagaaat atcaacatgt tatgtgtttt atttaattga atgggctgtt tttttatgga
      121 agaattgagg aaaacgtagg aatctgacat tttgaatcag aaggtactgt tttaacgagg
      181 cttttttttc cacacccctt taaatcgagc agcattactg gctgtgtatc tgacagtatg
      241 ctggatattt cgatgaccca gataatcctg aagtagtcgg gtatcgacac cattgtcagc
      301 cagagcaaaa ccacaggcat gacgtaacat atgaggatgt gcttttacag ctaatcctgc
      361 acgtcttcca gcctcacgaa tgatagaaaa aaaatgttgt ctgctgatag ggcggcgttt
      421 ccgggagaga aaaagccagt ctccttcaac tccgttttca attaacttcc tctgcttaag
      481 ccacaacttg atcaaattat attcatcagg aagaagagga tgtgttgtcg agaaaccatt
      541 tttaatacgc cgaatattaa gttgttttcc tgatgcatca atatcggata aacgtaaatc
      601 aagaagttca ctggccctaa aaccgtgaat gaaagccatc ataattagac agcgatttct
      661 ttcaggaaag gacatgctct gggctgcatc catcagcctg tagacttcat cctgtgtgag
      721 atattttctc gtcatacgcc accgtgtaca gttgtaaaaa agagaggcac acccagataa
      781 gtatgcctgc aatcagaacg ttatcgatgt aatctcatta tctcattaat aagatttttt
      841 gaatagtagt tctacgcaat agac
//
LOCUS       DAPGRN010000324          857 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPGRN010000324 DAPGRN010000000
VERSION     DAPGRN010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.330"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            136..>857
                     /locus_tag="RHW33_004899"
     CDS             136..>857
                     /locus_tag="RHW33_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648301.1"
                     /translation="MSIDFTPGMINTYHGDIYNRTTDTDNVKTPDTPMWPCDNREEQQ
                     PINSTFSGEGYNPERYDLARQHLPQIYACRMNTKYTDADYSKVVAQLVSLITNIESIS
                     STPLTRQAQEILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPVRFSV
                     QTMFSDTNFIAEQRADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNM
                     TGQNLSGFVLTLSRFYFEDLLN"
BASE COUNT          318 a          164 c          151 g          224 t
ORIGIN      
        1 aaaaggggag tcaaggtaat tatatcagga agatatgacg catgatattt attgtgcgta
       61 gaagaaaatc cattcctgct ctaattatcc aatttaaaaa tcttagcata aaacagatga
      121 tgaataagga atgttatgtc aattgacttt accccaggta tgataaatac atatcacggc
      181 gatatttata accgcacaac agataccgac aatgttaaaa caccagacac accgatgtgg
      241 ccttgtgata atcgggaaga acaacaaccc attaattcca cattctctgg agaaggatac
      301 aaccctgaac gatatgattt agcgcggcaa cacctcccac aaatttatgc gtgccgcatg
      361 aacaccaaat atacagatgc agactactca aaagttgtgg ctcaacttgt aagtcttatt
      421 actaacattg aatcgataag ttcgacacca cttacgcggc aggcacagga aatacttaat
      481 caaattaata acattagata cgaaaagaac aaaagtgctg aatgtcgaat cattgttgtc
      541 gctaatccta aacccgacaa ggcaatcata actaaaatta gcgtagaaga gggaatacca
      601 gtaagattca gtgtccaaac catgttttca gatactaatt ttattgctga acaacgagct
      661 gacttaccga ctaatataaa agatattcaa agcctgtatc aaaaaatgac gaagttatat
      721 attgagcaca gtgaaaataa aaacaggatg aaagtctttg ccggaaccaa ctttattgat
      781 ttcaatatga caggacagaa cctgtccggg ttcgttctaa ccttgtcaag gttttatttc
      841 gaagatctac ttaatat
//
LOCUS       DAPGRN010000325          855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPGRN010000325 DAPGRN010000000
VERSION     DAPGRN010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.331"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..792
                     /locus_tag="RHW33_004900"
     CDS             <1..792
                     /locus_tag="RHW33_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV1648302.1"
                     /translation="IQRITHNLWHARKKNVFLIEQITNLKEWVNKYIADCTDEELNDR
                     DFIASVVDRAIFHFAVNSICNTEGNKDATPIERYTFDVETKNGLPSTVQLFYEESKDN
                     EPLANIHLQAIGSGFLTFVNACQKYDDNSLKLFASLLISLSYSSAYTDLAGAEKVNIN
                     DHNGNYLTAQFEELSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLAPDIL
                     SKMRDAAPKLIDFAKSFDPNSKEKIKIYTGTSKLIYDLFGVKSEK"
BASE COUNT          289 a          127 c          177 g          262 t
ORIGIN      
        1 attcaaagaa tcactcataa tttatggcat gcacgtaaaa aaaatgtgtt tttaattgag
       61 caaataacta atctcaaaga gtgggttaat aagtatattg cagattgcac tgatgaggaa
      121 ttaaacgatc gtgactttat tgcatcagtt gttgaccggg ctatttttca tttcgcggtt
      181 aatagtatat gtaatactga gggtaataaa gatgcaacgc ccatagaacg atatactttt
      241 gatgtagaaa ccaagaatgg ccttccctcc acggttcagc tattttatga ggaatctaaa
      301 gataatgaac ctttagcgaa tatacatttg caagcaatag gttctggttt tttaacgttt
      361 gttaatgcat gtcagaaata tgatgacaac agcttaaaat tattcgcttc gctgttgatt
      421 tcactttcat actctagtgc ctacacagat ttagcaggag cagaaaaagt gaatattaat
      481 gatcataatg ggaactatct gacagctcag tttgaagagt tatctcaacg tgatatgaag
      541 aagtacctgg gagaaatgaa gcgtctggca gatgggggag aaatgaattt tgatggctat
      601 ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg cccctgatat tttaagcaaa
      661 atgcgagatg ctgcaccgaa attaattgac tttgctaagt cgtttgaccc aaactcaaag
      721 gaaaagatta aaatatatac aggtacttct aaattaattt atgatttatt cggggttaaa
      781 tcggagaaat aatatgtgaa gttcttcgat ggtatgaaag gtattacata aaagaaccca
      841 atgcttattg ggttc
//
LOCUS       DAPGRN010000326          847 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.332, whole genome shotgun sequence.
ACCESSION   DAPGRN010000326 DAPGRN010000000
VERSION     DAPGRN010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.332"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(108..671)
                     /gene="fimA"
                     /locus_tag="RHW33_004901"
     CDS             complement(108..671)
                     /gene="fimA"
                     /locus_tag="RHW33_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV1648303.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSNNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT          255 a          195 c          168 g          229 t
ORIGIN      
        1 cctcattaat aatgagattc acattggtga attgctcggg tgtgtgtagc catgccacac
       61 acaccctgtt tcaatttcag atgacgtata gccaccttaa gattgaatta tagatattga
      121 actttgaagg tcgcatctgc gtttgcagta ccagctacgg actgcccgag tgcaatataa
      181 cgagcctgga acggcagtat attcgtgcca tcaatcaagt cagttttagc gctaaaagtg
      241 gcaccatcaa gtattaatac ctcacccgta cggtccaaaa tttggatacc gacattttgg
      301 gcagagcctg ccgctgaact ctgcagcgct aacgtatcgt cattactggt gacagtagta
      361 cccaggaatg caacagctgc attattggaa acattggtat cacaatcatt cagcttgata
      421 ttgaagccaa cggcggagct gaggtcaccc gctttagcca ggcgagaaga acgaacctga
      481 cccagctcaa ccgtttggtt cattgattcg gaatcaatcg cacaagcggc atttaccact
      541 tcacctttaa aatgaataac accgccatta acagtggtcg ttactgattc atcaccctcg
      601 gctgcgctta ccgcagcaga cgacatcgcc aacacagaaa cgacaatcat accaacatgt
      661 ttgagtttca taattctcca aaatttatat ttaagtagct gaataaagag agttaacaat
      721 taagtcattc tgttattgct tataaaacaa gaatcattct tgattggttg ttgccctgag
      781 aatatagttt ccagggttat tacatttcac gaagaacgat gatatcttta aatcacaagg
      841 acaccta
//
LOCUS       DAPGRN010000327          845 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPGRN010000327 DAPGRN010000000
VERSION     DAPGRN010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.333"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(254..667)
                     /locus_tag="RHW33_004902"
                     /pseudo
     CDS             complement(254..667)
                     /locus_tag="RHW33_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            665..793
                     /locus_tag="RHW33_004903"
                     /pseudo
     CDS             665..793
                     /locus_tag="RHW33_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          240 a          203 c          193 g          209 t
ORIGIN      
        1 catgcaagat aagggaaggt tctatttatt gacattaata ccctcgacaa ttgctgagca
       61 gaagccccca cgacaagtga cagaatgaca acatctacag tggggagcgt tttatcgatg
      121 aacaagcaac caagaagtaa cacggcatag gtgacgacaa tgccagtaag catagaaata
      181 acaaaaacgt gagctttcat cattcctcca gaataacccc gacgtgtaag cctgggggat
      241 tggcacaata atgtcattta aaggcagact ttccggcaaa ttgcgccgat gaacccagtt
      301 catcctcaat tctcataagc tggttatatt tttcaatacg ctcaccccgg catggagctc
      361 ccgttttgag atgacctgcg cgcattgcaa ctgtcatatc ggcgataaag ctgtccactg
      421 tctcaccact gcggtgtgag ataaaggtgc cccagttgtt gtcatggcaa agttgtaccg
      481 catcaaaggt ttcactcagg gaaccaatct ggttgagttt aatcaatgct gaattggcaa
      541 ggttttcgtc aatgccgcgc tgaatatact ttacgttggt cacaaacaga tcgtcgccaa
      601 ccaattctat ctgctctccc aatgcaccat gcaggatttt ccatccagcc cagtcatctt
      661 cagctaccgt taagggcagg aagatcaact aggtgctctg gggctggtga ccaatgcggt
      721 tgtgctgtga aacacacttt acatgcagga ggcgttgtca catcttcgca aatcgggaga
      781 aactcctgag gagtagcatc cggcaagatt atcgctgctg atacatagcc atataaatat
      841 gctgg
//
LOCUS       DAPGRN010000328          842 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPGRN010000328 DAPGRN010000000
VERSION     DAPGRN010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.334"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            29..>842
                     /locus_tag="RHW33_004904"
     CDS             29..>842
                     /locus_tag="RHW33_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1648304.1"
                     /translation="MTNLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     HGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLP"
BASE COUNT          268 a          180 c          151 g          243 t
ORIGIN      
        1 tggaagaaaa atataaccgg agtagtgtat gaccaatctc tacgacttga aaaagttcga
       61 cttaaatctt cttgtcatat ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc
      121 tgaatcacta tatatcaccc catcagccgt gagtcagtcg ttacaacgct tacgtgcgca
      181 atttaatgac cctttattca ttcgctcagg aaaaggtatc gcgccgacga caacagggtt
      241 aaatctgcat caccatcttg agaaaaatct cagaggcctt gaacaaacaa ttaatatagt
      301 caataagtcc gaactaaaga aaaactttat tattcacggc cctcagctca tttcttgctc
      361 taataatagt atgctgatcc gctgcctacg gcaagattcc tctgtcgaga ttgagtgcca
      421 tgatatcctc atgtcagcag agaatgctga agaactgttg gttcaccgca aagccgatct
      481 ggtgatcacc caaatgccag tgatcagccg ttccgtcatt tgcatgccat tgcatacgat
      541 tcgcaatact ctaatttgta gtaataggca tccacgaatt actgacaata gcacttatga
      601 acaaattatg gcagaagagt tcacccaatt aatatctaaa tcagcagggg ttgatgatat
      661 tcaaatggaa atagacgaga gatttatgaa tcgcaaaatt tcatttcgtg gttcctcttt
      721 gctgacaatc attaatagca ttgcagttac agatttactg ggaatagtcc cctatgaact
      781 ttataactcc caccgtgatt ttcttaattt aaaagagata aaactagaac atccgttacc
      841 tt
//
LOCUS       DAPGRN010000329          833 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPGRN010000329 DAPGRN010000000
VERSION     DAPGRN010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.335"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            229..>833
                     /locus_tag="RHW33_004905"
     CDS             229..>833
                     /locus_tag="RHW33_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648305.1"
                     /translation="MSPDNIGSAVNRFSGWPSNDPLKTDEESMTSPVTVQVIKMIAAI
                     LDPHRIVVGIDTLYTTQRETFTSESQQLIESALQTLHVMNLNNLETNLDEEGHYLAFG
                     YNVDTPDRITVSAYNEKKPGCYGQCLLIQDDETDLFNLIKVKIFEARHNYVAQLMSDP
                     EFMFKFSYSAQQNRLEPHLLVPTYFPLKTNEPVTQEDILLL"
BASE COUNT          257 a          164 c          163 g          249 t
ORIGIN      
        1 attgttgcct ttatgagcat cgaccgtaag tccgttatgt tattgtttca ttagtaataa
       61 tcactcaaat gtatcgatgg aacaggtatg acttagcggg agtgattgtt tctgagtgaa
      121 cagaggccac tttcgcctga taaatgaaaa atgagcgatt tttgcaagtt agtttttcta
      181 tagccgagta cgctcagcgc ctcgcttgat tacaatgagg ttgctgctat gagtcctgat
      241 aatatcggtt cggctgtcaa tcgcttctca ggttggccat ctaatgatcc tctaaaaaca
      301 gatgaagaat caatgacatc acccgtgact gtacaggtaa taaagatgat tgccgccatc
      361 cttgatccac atcggatagt cgttggaatt gatacacttt atacaacgca gcgcgaaaca
      421 tttacctcag agagtcaaca gctcattgaa tcagcattgc aaacacttca tgtgatgaat
      481 ttaaataacc tggaaacaaa tctcgatgag gaaggtcact atctggcttt tggatataac
      541 gttgacactc ctgacagaat aaccgtttct gcttataatg aaaaaaagcc tggctgctat
      601 ggccaatgtc ttttaataca ggatgacgaa acagacttat ttaacctgat aaaagttaaa
      661 atttttgaag ccaggcataa ttatgtcgct caattgatga gcgatccgga atttatgttc
      721 aaatttagtt atagcgctca acaaaataga ttagaacctc atcttctggt tcccacttat
      781 ttccctctaa aaacaaacga accggttacg caagaggata tactgttact tta
//
LOCUS       DAPGRN010000330          826 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPGRN010000330 DAPGRN010000000
VERSION     DAPGRN010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.336"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            85..717
                     /locus_tag="RHW33_004906"
     CDS             85..717
                     /locus_tag="RHW33_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV1648306.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          266 a          137 c          162 g          261 t
ORIGIN      
        1 gcaaaattac aaaatcatgg tgtattgaca tcatcaataa ccgatgaaat tatgcaatta
       61 tatacggata gggaggttct taacatgggg ctttgtagtc gttataaaag tcttacatgc
      121 aatagttgct caatgcattg ccaaataatg ccagaagagt caccgcgttt gcagtattgc
      181 gcaaactcgt gtttttgtat gtggcccgaa gaaagttcat attttaatcg tggtgtggta
      241 gaaggtatct taacaaaaaa ccacaatgcg agattaagcg gatatatttt tgtcgatttt
      301 tcagtaagtt ttttacgtct attccttgaa aaagactgga ttgactatct tgcaagtact
      361 gacatgggga ttgttttagt cagcgaccgc aatatgcaat cgttagctaa ttactggcga
      421 aaacacaact ctgcaatatc tgctgtcata tataatgatg atggtcttga tgttgccaat
      481 gaaaagatca gacaactatt tattggtcgt tatttatcat ttaccagagg taacacatta
      541 actcagatgg aattcaccat aatggggtat atggtttctg gttataatcc atatcagatt
      601 gctgaagttc tggatatgga tatccgtagc atctacgcgt acaagcaacg aatcgaaaag
      661 agaatgggtg gtaaaataaa cgaattattt attcgttcac attcggtcca acattgatac
      721 tcaaaaatct gccagaatca tactctgttt atacgggtat ttattgaaat gcccgtattt
      781 tataaatagg ccgtcccctc aagagtaaac atcatagtat ttaaaa
//
LOCUS       DAPGRN010000331          816 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPGRN010000331 DAPGRN010000000
VERSION     DAPGRN010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.337"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..110)
                     /locus_tag="RHW33_004907"
     CDS             complement(<1..110)
                     /locus_tag="RHW33_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV1648307.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDD"
     gene            complement(107..541)
                     /locus_tag="RHW33_004908"
     CDS             complement(107..541)
                     /locus_tag="RHW33_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV1648308.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
BASE COUNT          214 a          185 c          205 g          212 t
ORIGIN      
        1 ttatcatcca gcacatgctg tatctgctcc cccagaccgt tgaaggattt acgcatatca
       61 gtaacgccgg caacgagcca gatacgggta cctgatggga gtgaaatcat cgtcccctcc
      121 cggtcagctc acggatcaac accgtgagca gctctggtga aggattttcc agcatcattt
      181 taccgtaatg gaattccaca tggcaggaag ctgcacaagc aactgataat gcctccggag
      241 caggagcatc tgtagcacag cgcggggtgt cgataagttc cactggaacc agagtcggag
      301 aggccggaaa agattgtgat actgtagggc ctacaatagt tggaggcatt ctacgagaaa
      361 tacgtccttc tctttgccag aggcgtagcc atttaaaaat caggttgtta tcaacgccat
      421 gttcccgggc cagttgtgca acattggcat ttggtttcga agccagttca accatatgaa
      481 gtttaaattc attagaaaat atccggcggg ttccggagcg ccagtctgta ggtttatcca
      541 tcaatagaac tccgtctaat ttagaaggag ctctaattta gtctgcaaag agcaggtgtt
      601 acagacggca ttggctttac gcttacgact atcacttatt taagtgatat tggttgtctg
      661 gagattcagg gggccagtct aaattccgta ttgctggttc tttatcggta gaaaaatcaa
      721 tatccatagc atcggtcgca ggcgctgata cattgcagag cgaccgggta ttagagtcgc
      781 tattcactgg ttatcactga acgctgcgtg atcagg
//
LOCUS       DAPGRN010000332          814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPGRN010000332 DAPGRN010000000
VERSION     DAPGRN010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.338"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            62..565
                     /locus_tag="RHW33_004909"
     CDS             62..565
                     /locus_tag="RHW33_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HDV1648309.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            597..>814
                     /locus_tag="RHW33_004910"
     CDS             597..>814
                     /locus_tag="RHW33_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648310.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIA"
BASE COUNT          246 a          121 c          205 g          242 t
ORIGIN      
        1 aactgatgaa aagagcaaag gagtatcagg ataagcacaa gcaataatat tgtatgggga
       61 gatgataatg aaaaatttag ctcatattgc tctggtgacg gtgatacagt tcattgcctg
      121 ttatctggct gattggggga gtgccgaaac agtttttatc ctgtgcttta ttgtactatg
      181 gcagggattg tttatatggt tattttctca aatacgaaaa aaacggcatg tgtcggatga
      241 gtttaaattc agtaaaggcg tttggtatat actgatgcca gtatcttcct tactctctcc
      301 gttattaagc ctgatggttt ttatcattgg tacactatac gaattaaggc gaattagtgg
      361 ttgtgtaagt gtaagagaat ggatgcaatc tcaggtaaat gaacaaacta atgaagattt
      421 gcatctggat tttgatgatg tgaattttga ttatgtggat ttttatcgta ctaatccggc
      481 aacaggatta gctatgcatg gaggatttga ttcagcagga aatacttttg ggacttgttg
      541 gcaggactat gatgacaggc gataagaaaa ctgtggtttg taataaaggt aatttgatga
      601 aaacaaaaaa attgatgatg gttgcactgg tcagttccac tctggccctt tcagggtgtg
      661 gtgcgatgag cacagcaatc aagaagcgta accttgaggt gaagactcag atgagtgaga
      721 ccatctggct tgaacccgcc agcgaacgca cggtatttct gcagatcaaa aacacgtctg
      781 ataaagacat gagtgggctg cagggcaaaa ttgc
//
LOCUS       DAPGRN010000333          814 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPGRN010000333 DAPGRN010000000
VERSION     DAPGRN010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.339"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>814
                     /locus_tag="RHW33_004911"
     rRNA            <1..>814
                     /locus_tag="RHW33_004911"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          224 a          176 c          252 g          162 t
ORIGIN      
        1 acgtggtatc ctgtctgaat atggggggac catcctccaa ggctaaatac tcctgactga
       61 ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa
      121 aagaacctga aaccgtgtac gtacaagcag tgggagcctc ttttatgggg tgactgcgta
      181 ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga ataggggagc
      241 cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc gaaacccggt
      301 gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg aaccgactaa
      361 tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat
      421 agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga
      481 gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg
      541 gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca
      601 acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc
      661 ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca
      721 ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc gaagctgcgg
      781 cagcgacact atgtgttgtt gggtagggga gcgt
//
LOCUS       DAPGRN010000334          811 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPGRN010000334 DAPGRN010000000
VERSION     DAPGRN010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.340"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..389)
                     /locus_tag="RHW33_004912"
     CDS             complement(<1..389)
                     /locus_tag="RHW33_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1648311.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIR"
     gene            complement(437..>811)
                     /locus_tag="RHW33_004913"
     CDS             complement(437..>811)
                     /locus_tag="RHW33_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV1648312.1"
                     /translation="IIRVNKSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAE
                     EAAVASQAAKRPPVVNYPGEGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTM
                     DNNFRLVNVYITDMKITEIPQK"
BASE COUNT          151 a          249 c          214 g          197 t
ORIGIN      
        1 tgccggatat cctgcccgtg gctgaacggc ggattcatga taacccggct gtaatactgc
       61 actggctgcc attccatgaa gtcgccacac tgaaccctga caccgttaaa attttcccgc
      121 agataccgga ccagcccgct gttgatttcc accgcatcac acatggcccc cggcgcggta
      181 tcccgaatcg cccgcagtat ggccccggtg ccggcagagg gttccagaat gtgatcacgg
      241 ttgctgatct ccgccagcat caccaggcga tcacagaccg gggcaggagt cacaaaaagc
      301 tgagattcag cccggatttc agcaaccggc gcggcgttat ccagccttgc gcgcatcagc
      361 cggatacgtt ctcttaactg actgtgcata tcattatctc cgtcatcccc cggggatttt
      421 cccgggggga tataccttat ttctgtggga tttccgtgat tttcatgtcg gtgatataca
      481 cattcaccag gcggaaatta ttgtccattg tgcggcggta ccggtatgcc ccgtggtctt
      541 ctgcttctgc cacactgcgc acggccttac agtcccgggg cagggcggcc cactgtgcct
      601 ttgtcatttc ccggaaacct tcccccggat agttgactac cggcggacgc ttcgcggcct
      661 gactggcgac ggcagcctct tctgccgatg gtgctttgta gtccgtgatg cgatcgggcg
      721 tcactttcat cgtgccgctg tacccgagaa aactgtaatt tggcgttgtg actgaactca
      781 ccgccccgtt gcttttgttc acacggatga t
//
LOCUS       DAPGRN010000335          810 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPGRN010000335 DAPGRN010000000
VERSION     DAPGRN010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.341"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..>810
                     /locus_tag="RHW33_004914"
     CDS             39..>810
                     /locus_tag="RHW33_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1648313.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVAG"
BASE COUNT          264 a          157 c          160 g          229 t
ORIGIN      
        1 gtgatttcca tttcgattga tttagtgttt attgacgtat gtactggatt attaacgata
       61 atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc gatcttaacg
      121 tcgttctgac aacgccagcc agtcgttgtt tatcacttct acttgaagct tttcctgatg
      181 tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg cgtgtgccta
      241 ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct gttggtgata
      301 ctacccactc gcttattgca accgtgccga gaagaggatt caagatccat aatgacatta
      361 acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat gccccacctg
      421 ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat ttcaataaca
      481 aaatcctcaa acatataaaa agttatctaa ttatgttgag cgcatttgtc ataggcgcat
      541 attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag gattacaaga
      601 ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt gatgggttga
      661 aagagttcga taaatataaa acacggatac tggattcggg gattaactgt aaaaaatatc
      721 cgtggttata cttccctctt gcaaaatcct caccggggat gattgttatg gcatgcaata
      781 aaaactataa ccaacatgaa gtggcaggct
//
LOCUS       DAPGRN010000336          793 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPGRN010000336 DAPGRN010000000
VERSION     DAPGRN010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.342"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            197..763
                     /gene="slp"
                     /locus_tag="RHW33_004915"
     CDS             197..763
                     /gene="slp"
                     /locus_tag="RHW33_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV1648314.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          242 a          165 c          177 g          209 t
ORIGIN      
        1 attttaaaac aaagggttat attatggggc gatggatact catgccacgc cccaaaataa
       61 cggaattgaa ctaaacagaa gggatataaa catcagacag gtttacgtta ctatcaggca
      121 tatcgcctca gaatcagatg aaaactataa agaaatatct attatggttt taatatttgt
      181 tgataaggat agtaacatga acatgacaaa aggtgcactc atcctcagcc tttcattttt
      241 gcttgccgca tgtagttcaa ttccgcaaaa tatcaaaggc aataaccaac ctgatattca
      301 aaaaagtttt gttgctgttc ataaccagcc ggggttatat gttggtcaac aagcgcgctt
      361 tggtgggaag gttatcaacg ttatcaatgg caaaacggat acgttgttag aaatcgctgt
      421 attaccgttg gatagctatg cgaagcctga tattgaagcc aactatcagg gccgactgct
      481 cgccagacaa agcggcttcc ttgacccagt gaactatcgt aatcactttg ttaccatcct
      541 cggtaccatt cagggtgaac aaccaggctt tatcaataaa gtcccgtata acttcctgga
      601 agtgaatatg cagggcatcc aggtgtggca tttgagagaa gtggttaata ccacctataa
      661 cctgtgggat tacggctatg gtgcattctg gccggaaccg ggctggggtg cgccttacta
      721 caccaatgcg gtgagtcagg taacacctga gctggtcaaa taacaccacc gaaagatgca
      781 gatgctttct cag
//
LOCUS       DAPGRN010000337          778 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPGRN010000337 DAPGRN010000000
VERSION     DAPGRN010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.343"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(32..736)
                     /locus_tag="RHW33_004916"
     CDS             complement(32..736)
                     /locus_tag="RHW33_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV1648315.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          178 a          205 c          200 g          195 t
ORIGIN      
        1 atgaggcagc cttttgtctt attcaaaggc cttacatttc aaaaactctg cttaccaggc
       61 gcatttcgcc caggggatca ccataataaa atgctgaggc ctggcctttg cgtagtgcac
      121 gcatcacctc aatacctttg atggtggcgt aagccgtctt catggattta aatcccagcg
      181 tggcgttgat tatccgtttc agtttgccat gatcgcattc aatcacgttg ttccggtact
      241 taatctgtcg gtgttcaacg tcagacgggc accggccttc gcgtttgagc agagcaagcg
      301 cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg tgggatctgc cacttcttca
      361 cgttgttgag gattttaccc agaaaccggt atgcagcttt gctgttacga cgggaggaga
      421 gataaaaatc gacagtgcgg ccccggctgt cgacggcccg gtacagatac gcccagcggc
      481 cattgacctt cacgtaggtt tcatccatgt gccacgggca aagatcggaa gggttacgcc
      541 agtaccagcg cagccgtttt tccatttcag gcgcataacg ctgaacccag cggtaaatcg
      601 tggagtgatc gacattcact ccgcgttcag ccagcatctc ctgcagctca cggtaactga
      661 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
      721 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcacca
//
LOCUS       DAPGRN010000338          761 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPGRN010000338 DAPGRN010000000
VERSION     DAPGRN010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.344"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..747
                     /locus_tag="RHW33_004917"
     CDS             31..747
                     /locus_tag="RHW33_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV1648316.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          254 a          131 c          164 g          212 t
ORIGIN      
        1 tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa tattgttgct
       61 aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg tgtagaaatc
      121 tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt aaaattcgtt
      181 attgaacgtg caaagcaggc acaagttcca aaacacgtta ttgataaagc aattgataaa
      241 gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt tggtcctaat
      301 ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat tgctaacgtt
      361 cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt cagctatatg
      421 tttgacaata ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat ttttgaaatt
      481 ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa cattgttatt
      541 tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc tggaatcacg
      601 gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct ttccccagaa
      661 gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga tgtacaaaaa
      721 gtttatcata acgtcgcaaa tctctaatta tattttaaag a
//
LOCUS       DAPGRN010000339          747 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPGRN010000339 DAPGRN010000000
VERSION     DAPGRN010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.345"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>747)
                     /locus_tag="RHW33_004918"
     CDS             complement(<1..>747)
                     /locus_tag="RHW33_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV1648317.1"
                     /translation="QHELVSDDAIINELSSELVSCLGNGKFTPISEGSKLLNMLSEFK
                     LLREQCFRWGNYTLLFENYGAYDKTGSITIEKSQGEGTLPIRHKLEFISTSIAELLDK
                     LTKITDARLCKGFSDWASSVKEGGSNDLKENVDRALVRMFKCVKLHSNELNLSYLFLG
                     SVPPLPEWIEMLSLIHNKLDSIQVPESCKELELDFNNLTEFPQVPDGITLISVNNNLI
                     SHIDSFPPKVKKIFIGHNKLSEIPAIPDTAK"
BASE COUNT          219 a          157 c          123 g          248 t
ORIGIN      
        1 cttagcggtg tctggtattg ctggtatttc cgatagctta ttgtgaccaa taaaaatttt
       61 cttaaccttt ggcggaaatg agtcaatatg cgatatcagg ttattattta cggagatcag
      121 ggtaattcca tcaggtactt gtggaaattc tgtaaggtta ttgaaatcga gttctaattc
      181 tttgcatgat tcgggcacct gtattgaatc aagtttatta tgaataaggc taagcatttc
      241 aatccactct ggaagaggcg gcacagaacc caaaaaaagg tatgataagt taagttcatt
      301 actgtggagc ttaacacatt taaacattct caccaatgct ctatccacat tttctttcaa
      361 gtcattcgat ccgccttctt tgactgaact agcccagtca ctgaaacctt tgcaaagtct
      421 ggcatctgta attttggtta acttgtccag caactctgca atactggtcg atataaactc
      481 taatttatgc cgaatgggta aagtcccctc cccctgactt ttttctatcg tgatagatcc
      541 cgtcttatca taagccccat aattttcaaa caatagagta taattacccc acctgaaaca
      601 ttgctctctc aataacttaa attctgacag catattcaat aatttgctgc cttcactaat
      661 tggtgtaaac ttaccatttc caagacaact gactaactca cttgataatt catttatgat
      721 tgcatcatca gatactaact cgtgttg
//
LOCUS       DAPGRN010000340          744 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPGRN010000340 DAPGRN010000000
VERSION     DAPGRN010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.346"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            4..>744
                     /locus_tag="RHW33_004919"
     CDS             4..>744
                     /locus_tag="RHW33_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDV1648318.1"
                     /translation="MNNKKLKFVIGDTRCYESINEAMRGVDYVFHAAALKQVPSCEFY
                     PMEALKTNVIGTENVLNAAINNEVKKVVLLSTDKAVYPINAMGISKAMAEKILVAKSR
                     IVGSSGPIMCATRYGNVMASRGSVIPLFVEKIKQGKPLTITDPNMTRFLMSLEDSVDL
                     VLHAFAYGQQGDIFVQKAPASTVNELSVALCDIFNSQLNYKIIGTRHGEKLYETLVSR
                     EEMAKAQDMGRYFRIPADNRDLNYDKYII"
BASE COUNT          253 a          109 c          158 g          224 t
ORIGIN      
        1 gctttgaata ataaaaaact aaaatttgtt attggtgata ctcgctgtta tgaaagtata
       61 aatgaagcta tgagaggcgt agattatgtt tttcatgctg cagccctgaa acaagtgcca
      121 tcgtgcgaat tctatccaat ggaggcctta aagacaaatg tcattgggac agaaaacgta
      181 ttgaatgctg caattaataa tgaagtaaaa aaagttgttt tgttaagtac agacaaggct
      241 gtatatccta ttaatgcaat gggaatatcg aaagcaatgg cggaaaagat tttagttgct
      301 aaatcacgga tcgttggaag tagtggacca attatgtgtg ctaccagata tggtaatgta
      361 atggcgtcaa gaggctccgt tattcctctt tttgtcgaaa aaattaagca aggcaaacca
      421 ttaaccatta cagatcctaa tatgactcgg tttttaatgt ctttagagga ttctgttgac
      481 ttggttctcc atgcttttgc ctatggacag caaggtgaca tttttgttca aaaagctcct
      541 gcaagtaccg ttaatgaatt atctgttgcg ctatgtgata tttttaattc gcagttgaat
      601 tataaaataa tcgggaccag acacggagaa aaactttatg aaacactagt atcaagagag
      661 gaaatggcaa aagcacaaga tatggggcgc tactttagaa taccggcaga taatagggat
      721 ttaaattatg ataagtatat aata
//
LOCUS       DAPGRN010000341          741 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPGRN010000341 DAPGRN010000000
VERSION     DAPGRN010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.347"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>741)
                     /locus_tag="RHW33_004920"
     CDS             complement(<1..>741)
                     /locus_tag="RHW33_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV1648319.1"
                     /translation="WQRWISRKMSLAEEIKKGMSSGEFSVHYQPVCDTTTKACLGVEA
                     LMRWQREDGKNISPVVFIRAAEEENLIIPLTKHLFELIIQDVQSWKVKKPFHLGINIA
                     AEHLAHPDFVADVLHIKNAISDKFNIVLEITERNLVEDTDLALQKINELRIHGCEFAV
                     DDFGTGYCSLGLLQKLSVDYLKIDKSFIDTLTTAEDETPVLDIIIKLSNRLNLITIAE
                     GVSTPHQAEYLIHNNVTLVQGYLYAKPM"
BASE COUNT          220 a          158 c          126 g          237 t
ORIGIN      
        1 ttcattggtt ttgcatataa gtatccctga actaatgtca cgttattatg aataagatat
       61 tccgcctggt gaggagtact gacaccttct gcaatcgtta tcaaattgag tcgattactc
      121 agcttaataa taatatctaa aactggcgtt tcgtcttccg ctgtagtgag tgtgtctata
      181 aagcttttat ctattttaag ataatcaaca gacaactttt gcagaaggcc aagggaacaa
      241 tatcccgtgc caaaatcatc aactgcaaat tcacagccat ggatccttaa ttcatttatt
      301 ttctgtaatg caagatcagt atcttcaacc agattgcgtt cggtaatctc aagaacaatg
      361 ttaaatttat cagatattgc atttttaata tgtaaaacat cagcaacaaa atcaggatgt
      421 gcaagatgct ccgcggcaat atttatccca aggtgaaacg gctttttcac tttccagctt
      481 tgtacatcct gaataatcag ttcaaagaga tgcttagtta acggtataat taaattttcc
      541 tcttctgcag cgcggataaa aactaccgga gatatatttt taccatcttc gcgttgccaa
      601 cgcattaacg cctcaacccc cagacacgct ttggttgtcg tgtcacatac tggttgataa
      661 tgcacagaaa attctccgga tgacattcct ttcttaattt cttctgccag ggacattttc
      721 cgagatatcc agcgctgcca t
//
LOCUS       DAPGRN010000342          722 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPGRN010000342 DAPGRN010000000
VERSION     DAPGRN010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.348"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            77..415
                     /locus_tag="RHW33_004921"
     CDS             77..415
                     /locus_tag="RHW33_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648320.1"
                     /translation="MKNVENIIWHDGVLVETRMECHHTEYNFILTAELYVDEFTAGRE
                     RKRFEFTGVEHLSQVMDIAELIDNQNAGNIKDATIVYKAKKYKISLHLFGGFLSFTFK
                     TMRVIDNECA"
     gene            402..>722
                     /locus_tag="RHW33_004922"
     CDS             402..>722
                     /locus_tag="RHW33_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648321.1"
                     /translation="MNVRNVKFDNLSFYEEKIRWRMRRQISNAEAIFYENQEEHIQRF
                     LGRYTCRKPVLVYWESEQRWTSVNGLEVMSMFDEHCHHIYLDDIKKKIQTELPENKTL
                     DFKRQ"
BASE COUNT          250 a          105 c          152 g          215 t
ORIGIN      
        1 ttgttaacga gagtatctgc gtcatcgaac cagtttagaa actctaatgg ttacttacct
       61 gagaatggtt aatagcatga aaaatgtaga aaatattatc tggcatgatg gtgttttagt
      121 agaaaccaga atggaatgtc accacaccga gtataatttt atattgacgg cggagcttta
      181 tgttgatgaa tttacagccg gaagggaacg aaaaaggttc gagtttacag gagtggaaca
      241 tttatcgcag gttatggata ttgctgaact tatagataac caaaatgcag gtaatattaa
      301 agatgcgaca attgtttata aggcaaagaa gtataaaatt tcacttcatt tgtttggcgg
      361 ttttttaagt tttacattta aaaccatgcg agttattgac aatgaatgtg cgtaatgtca
      421 aattcgacaa cttgtctttt tatgaagaaa aaatccgctg gcgaatgcgt cggcaaatct
      481 cgaatgcaga ggcaatattt tacgagaatc aggaagaaca cattcagcgc tttttaggca
      541 gatatacctg tcgcaaaccg gttcttgttt attgggaaag tgaacaaaga tggacatctg
      601 taaacggtct ggaagtgatg agtatgtttg atgaacattg tcatcacatc taccttgatg
      661 atatcaagaa aaaaatacaa actgaattac ctgaaaataa aactttagat ttcaaacgtc
      721 aa
//
LOCUS       DAPGRN010000343          721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPGRN010000343 DAPGRN010000000
VERSION     DAPGRN010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.349"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            107..703
                     /gene="fimE"
                     /locus_tag="RHW33_004923"
     CDS             107..703
                     /gene="fimE"
                     /locus_tag="RHW33_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV1648322.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          184 a          140 c          179 g          218 t
ORIGIN      
        1 atcacttcgt gtgtggttat ctttttatct attgggctaa ttttgaccga ttgaggtttc
       61 ctataggtat tcattcaaat atatctcagt taggagtact actattgtga gtaaacgtcg
      121 ttatcttacc ggtaaagaag ttcaggccat gatgcaggcg gtttgttacg gggcaacggg
      181 agccagagat tattgtctta ttctgttggc atatcggcat gggatgcgta ttagtgaact
      241 gcttgatctg cattatcagg atcttgacct taatgaaggt cgaataaata ttcgccgact
      301 gaagaacgga ttttctaccg ttcacccgtt acgttttgat gagcgtgaag ctgtggaacg
      361 ctggaccctg gaacgtgcta actggaaagg cgctgaccgg accgacgcca tatttatttc
      421 tcgccgcggg agtcggcttt ctcgccagca ggcctatcgc attattcgtg atgccggtat
      481 tgaagctgga accgtaacgc agactcatcc tcatatgtta aggcatgctt gcggttatga
      541 actggcggag cgtggtgcag atacccgttt aattcaggat tatctcgggc atcgaaatat
      601 tcgccatact gtgcgttata ccgccagtaa tgctgctcgt tttgccggat tatgggaaag
      661 aaataatctc ataaacgaaa aattaaaaag agaagaggtt tgatttaact tattgataat
      721 a
//
LOCUS       DAPGRN010000344          719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPGRN010000344 DAPGRN010000000
VERSION     DAPGRN010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.350"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            34..719
                     /locus_tag="RHW33_004924"
                     /pseudo
     CDS             34..719
                     /locus_tag="RHW33_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    315..431
                     /locus_tag="RHW33_004924"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          182 a          176 c          213 g          148 t
ORIGIN      
        1 gagactaaac ttcctgagaa agaggtaaac aggatgacta aaaatactcg tttttccccc
       61 gaagtccgtc aacgggcagt ccgtatggtt ctggaaagtc agagcgaata tgactcacaa
      121 tgggcgacaa tttgttccat tgctccaaag attggctgta cgccggagac tctgcgtgtc
      181 tgggttcgcc agcatgagcg ggataccggg ggcggtgatg gagggctcac caccgctgaa
      241 cgtcagcgtc tgaaagagct ggaacgtgaa aatcgtgaac tgcgccgcag taacgatatc
      301 cttcgccagg cttccgctta ttttgcgaag gcggagttcg accgcctctg gaaaaaatga
      361 tgccactgct ggataagctg cgtgagcagt acggggtcgg accgctatgc agcgaactgc
      421 atattgcccc gtcaacgtat taccactgtc agcaacagcg acatcatccg gataaacgca
      481 gtgcccgtgc gcagcgcgat gactggctga agaaagagat acagcgcgta tacgatgaaa
      541 atcacaaggt atacggtgtg cgtaaagtct ggcgtcagtt gttacgggaa ggtatcagag
      601 tggccagatg cactgtggca cgtctcatgg cggttatggg acttgccggt gttctccggg
      661 gtaaaaaggt ccgtacgacc atcagccgga aagccgttgc cgcaggcgac cgcgtaaac
//
LOCUS       DAPGRN010000345          707 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPGRN010000345 DAPGRN010000000
VERSION     DAPGRN010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.351"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            60..671
                     /locus_tag="RHW33_004925"
     CDS             60..671
                     /locus_tag="RHW33_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648323.1"
                     /translation="MRDSEKIEEGVSTTEFVVGYGAISMVKKSESMAAKGLVGLERVD
                     LIREVVFENGHYDWGCVVNDVLPNICKGNARIPIFHFLNHAKLINPDGSIINETELSG
                     GVLSRLKITPISFQSQGWDKRRSETVPEVKVGVNELYLAYDFSFFLRMMPYMELGLIK
                     RDMGELLKILKKHIDEAMNTQVLSSNFCKLVCIYDYIKNSHRY"
BASE COUNT          228 a           94 c          164 g          221 t
ORIGIN      
        1 tgataaaaga tgaactttat gaattagtct taaccgatga tcctaaagga aaattatatg
       61 tgagggactc agagaagatt gaagagggcg tcagtacaac agagtttgtt gtagggtatg
      121 gtgctatttc gatggtaaaa aaatcagagt cgatggcggc aaaaggattg gttggactgg
      181 agcgtgtaga tctcatccga gaggttgtat ttgaaaatgg acattatgat tggggatgtg
      241 ttgttaatga tgttctgcca aatatatgca aaggcaatgc gcgcattcct atttttcact
      301 tcttaaatca cgctaaatta attaatcctg atgggagtat aattaatgaa acagaactct
      361 caggtggcgt tttatctcga ttaaaaatca ctcctattag ttttcagtct caaggatggg
      421 ataaacgacg ctctgaaacc gttcctgagg tcaaggtggg ggtcaatgag ttatatcttg
      481 cttatgattt cagtttcttt ttaagaatga tgccttatat ggagcttgga ttaatcaagc
      541 gcgatatggg agagcttctc aaaatattga aaaaacatat agatgaagct atgaatactc
      601 aggtgttgtc aagtaatttt tgcaaactag tgtgtattta tgattatatc aaaaatagtc
      661 acaggtatta agtaagatat aaccttctta ggggacgaga tagtaaa
//
LOCUS       DAPGRN010000346          705 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPGRN010000346 DAPGRN010000000
VERSION     DAPGRN010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.352"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(178..678)
                     /locus_tag="RHW33_004926"
     CDS             complement(178..678)
                     /locus_tag="RHW33_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDV1648324.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLATFDDERDFFAACRSL
                     HQDEADPELMFQDYEGFPGNMASECHINWAYVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT          185 a          224 c          176 g          120 t
ORIGIN      
        1 ccccgcctcc ggaaatcaga acaacagaaa cgacataaca gaaaaatgcc tcacaccatg
       61 acgggcagga agggcagcgc cgcaggcgcg gccctcctgc atttcaggca ggcgtaagcc
      121 tgccagggtt ttccggcggc ctggcaggcc atacccccgc cggagcgggg gagtaaatca
      181 ccgacggaac acatgaccgt caataaaggt gaaggagtcc aggaataaat cccgcgcata
      241 cgcctcatag tcaaaataga gcgccaccgt ctccggcacg tcagccagca ggccggtatc
      301 actggcgaac tcaaccgcaa aagcctcctc actgtcagcc tcgccccacc aggcatcgcg
      361 gaaggtgtca aaatccgtct caccggtatc atccacccag agacgataag cctcttcgca
      421 gccttcatcc cgcgcctggc ggaagccttc aacataggcc cagttgatat ggcattctga
      481 ggccatattc cccgggaatc cctcataatc ctgaaacatc agttcaggat cggcttcatc
      541 ctggtgaaga gagcggcagg cggcgaaaaa gtcgcgttca tcatcaaacg tggccaggtc
      601 aaaccagcgg ccagcgatgc ttccacagtt gtatttgtgc caggttccaa cgtatacagc
      661 aggtgcaaca acagacattc ccgaatcctc catagtcaca tgttc
//
LOCUS       DAPGRN010000347          677 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPGRN010000347 DAPGRN010000000
VERSION     DAPGRN010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.353"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          228 a          119 c          132 g          198 t
ORIGIN      
        1 tttcctctga aacagagaga ttcaagataa agtttcaaca agaaaataga cttaatgatg
       61 taattctagg tatcagtcgt tggctaaaag aaaatcaagt tatagtggaa actattaaga
      121 aaattgatac tgaataacgt ggcgaataaa taaacggatc aagtcgttcc tgtgacaatg
      181 aggttatacg aaaagacgct tactcatcag aaatctggta tgctttaatt aaaccagtgt
      241 cgttgatgtg taagcgttga cgttgggaga cgattaaatg gttagaccac tcctacgcct
      301 tttccagacg gcgtataaga gtgcaacatg ttgagttttc cgagcgaatg gtgcatttta
      361 ctaaccgagt catttgaaac aaccaacgca ataaaaaaga ctggaataat aagcctgtat
      421 ttattattca tcgcaatcgc actctacggt tttatcctgc agaaatgaga ctacttccag
      481 cggttgtgga ttatccctgg gcgatagtca tagtctggtg tcaggattca caaatgacct
      541 gttacctgac atggatataa acacaacata gagtgcggta gttaatctga atcatctatt
      601 tttcggctac acagcacaaa acaacattat tgattcttta taagagagaa tctctccatc
      661 atttttatat aaatcat
//
LOCUS       DAPGRN010000348          669 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPGRN010000348 DAPGRN010000000
VERSION     DAPGRN010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.354"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..252
                     /locus_tag="RHW33_004927"
     CDS             <1..252
                     /locus_tag="RHW33_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648325.1"
                     /translation="SQIYSCSDRTLNDYAVIFIRNALNASEHIIIFNCTGWSDLNNES
                     AVMQFARVARKSGKQLIVAVREQDMKKMEAEFGRIIKIH"
     gene            475..>669
                     /locus_tag="RHW33_004928"
     CDS             475..>669
                     /locus_tag="RHW33_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV1648326.1"
                     /translation="MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFLLIFRE
                     RLCELNIHRGNTVVQVTLAYE"
BASE COUNT          188 a          128 c          176 g          177 t
ORIGIN      
        1 agccagattt acagttgttc tgacagaaca ttaaatgatt atgctgttat ttttatcagg
       61 aatgcgctga acgccagtga gcatattatt atttttaatt gtacaggatg gtctgatttg
      121 aataatgaat ctgccgtcat gcagtttgcc agagtggcac gaaaatcagg aaaacaactg
      181 atagtcgcag taagagaaca ggatatgaaa aaaatggaag cagagtttgg aaggatcata
      241 aaaatccact gacgccttcg cgttgctcag ttgtccaacc ccggggacgg gaaaaagcaa
      301 gttttccccg cccctggcgt ttctgtagtg aaaagcggga aatggaggga atggtcagta
      361 acagggaatg tggtattatc gcggcgggtg tctgagcctt tctggttcag gcaagacgca
      421 ggtaccagaa atgcgaagac cccacttgtt aatccattaa ctcgtgaggc ctgcatgaag
      481 taccttaaca ctactgattg tagcctcttc cttgcagaga ggtcaaagtt tatgacgaaa
      541 tatgccctta tcggggtgct cgccgtgtgc gccacggtgt tgtgtttttt actgatattc
      601 agggaacggt tatgtgagct gaatattcac aggggaaata cagtggtgca ggtaactctg
      661 gcctacgaa
//
LOCUS       DAPGRN010000349          667 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPGRN010000349 DAPGRN010000000
VERSION     DAPGRN010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.355"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            136..561
                     /locus_tag="RHW33_004929"
     CDS             136..561
                     /locus_tag="RHW33_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV1648327.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
BASE COUNT          192 a          152 c          161 g          162 t
ORIGIN      
        1 catttctatg gttgcatcac ttttaataaa aaaacgataa ataaataatc gtaagcgtaa
       61 actgaccgcc gtatgtagcc atcagacgag aattggtaac ttagacgccc atctgatata
      121 gacggacatc taagcatgga attacaggac tggcgaaaag aacctcgtaa aaactattcg
      181 aatgaattca aacttcgtat ggtggaactg gcatcacaac ctggagcttg tgttgcacag
      241 attgcacgtg aaaatggcgt caatgataat gttattttca aatggctcag gctctggcag
      301 aacgaagggc gtgtttcacg gcgtcttccg gtaacgacct cttctgacac tggcgttgaa
      361 ttattacctg tagaaataac gccggatgag cagaaagaac ctgtggcggc cattgcgccg
      421 tctttatcca cttccactca gaccagagtc agtgccagtt cctgcaaggt ggaattccgt
      481 cacggtaaca tgacgctgga aaatccatcg ccagagctgc tcacggtgtt gatccgtgaa
      541 ctgaccggga ggggaagatg atctcactcc catcaggtac ccgtatctgg ctcgttgccg
      601 gcgttaccga tatgcgtaaa tccttcaacg gactgggaga acaggtacaa catgtgctga
      661 atgataa
//
LOCUS       DAPGRN010000350          666 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPGRN010000350 DAPGRN010000000
VERSION     DAPGRN010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.356"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..231
                     /locus_tag="RHW33_004930"
     CDS             <1..231
                     /locus_tag="RHW33_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine Rich Repeat family protein"
                     /protein_id="HDV1648328.1"
                     /translation="EETIFSDAELKAILPMRRMYGVGDYKSNSSSLSSHSGLKDPTGT
                     PVCYYIHNESEPSLGYGPTCNNWLSQSFTTEL"
     gene            294..>666
                     /gene="espR1"
                     /locus_tag="RHW33_004931"
     CDS             294..>666
                     /gene="espR1"
                     /locus_tag="RHW33_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector leucine-rich repeat protein EspR1"
                     /protein_id="HDV1648329.1"
                     /translation="MKFPSIFNKIKPQSIQQHPEKNQLNWMLELNKWKEERILTGEIH
                     RPECRNEAAKRINCAFLSKQNDIDLSGLNLSTQPPGLQNFTSINLDNNQLTHFDATNY
                     DRLVKLSLNSNALESINIPQGR"
BASE COUNT          237 a          141 c          109 g          179 t
ORIGIN      
        1 gaagaaacaa tattttctga cgcagaatta aaagctattc tgcctatgcg tcgcatgtac
       61 ggggttggtg actataagtc aaattcctct tctctatcct cacactcggg gctcaaggac
      121 ccaacgggca cacccgtctg ttattatatt cataatgaga gtgaaccttc cttaggttat
      181 ggtccaacat gcaataattg gttaagccaa tcctttacaa cagagttata aaacacatcc
      241 tggtttatca agggtgataa atcagaaaaa cattgtatta ctacggagca gttatgaaat
      301 tcccttcaat atttaacaaa ataaaaccac aatccataca gcaacatcca gaaaaaaatc
      361 aacttaactg gatgctcgaa ttaaataaat ggaaagaaga acgtatactt acaggtgaaa
      421 tccatcgtcc ggaatgtcga aacgaagccg ctaaaaggat aaactgtgct tttttgtcga
      481 aacagaatga cattgattta tcaggactta atttatctac tcaaccacca gggctgcaaa
      541 acttcacctc tatcaatctt gataataacc aactcacaca ttttgatgca accaactacg
      601 atagactcgt aaaacttagt ctgaatagta acgctcttga gtcaataaat attcctcaag
      661 gcagaa
//
LOCUS       DAPGRN010000351          666 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPGRN010000351 DAPGRN010000000
VERSION     DAPGRN010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.357"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            93..>666
                     /locus_tag="RHW33_004932"
     CDS             93..>666
                     /locus_tag="RHW33_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDV1648330.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQIN"
BASE COUNT          203 a          128 c          160 g          175 t
ORIGIN      
        1 tgatttaaac aatataaatt catgaatata agcatatgat attaaaatca aatcagcaaa
       61 aacttgtttt tgcgtagtgt gtggtgcttt tggtggtgag aaccaccaac ctgttgagcc
      121 tttttgtgga gtgggttaaa ttatttacgg ataaagtcac cagaggtgga aaaatgaaaa
      181 aatggatgtt agcaatctgc ctgatgttta taaatgggat ctgcgaagcc gccgattgct
      241 ttgatcttgc aggtcgggat tacaaaatag acccggattt actgagagcg atatcatgga
      301 aagaatcccg ttaccgggtt aatgccatcg gtattaatcc ggtaacggga tatggcagcg
      361 gactgatgca ggtagattcc cagcatttta acgaactggc ccgctatgga attaagccgg
      421 aacatctgac aacagatccc tgtatgaaca tttataccgg tgcttattat ctggcaatag
      481 cctttaaaaa atggggcgtc tcctgggagg ccgttggtgc atacaatgcc ggattcagga
      541 agaccgaacg ccagaaccag agacgtcttg cctacgcatc agaggtttac cggatttata
      601 cctggataaa gagcagtaaa ggcatccggg ttccgaccac gaagaaatca ctttcccaaa
      661 ttaaca
//
LOCUS       DAPGRN010000352          664 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPGRN010000352 DAPGRN010000000
VERSION     DAPGRN010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.358"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>664
                     /locus_tag="RHW33_004933"
     rRNA            <1..>664
                     /locus_tag="RHW33_004933"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          158 a          154 c          215 g          137 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacagaa ctttccagag atggattggt gccttcggga
      541 actgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gact
//
LOCUS       DAPGRN010000353          664 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPGRN010000353 DAPGRN010000000
VERSION     DAPGRN010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.359"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          219 a          130 c          134 g          181 t
ORIGIN      
        1 ttcccacaga cagcatatgg cgatgctcaa ttactgcaag gttgttatga ctacatggat
       61 gcctttaagc gggtgatgga cagttcctca aaaaattcag atggattaca tctgtctgca
      121 ataccccggg tattttcggt ttgcgaaatg gatggaacaa cttgctcggg ggatagctga
      181 tggagttatt gaagtgccga aggcccccta aaaatcacag ccggaaagtt cgacggttcc
      241 ggcgtagacc acctggtact gtttttgtca tgtatcacct atgactgaga atttactcac
      301 ttagtcacgt atccattatt gcttggtaag gtcacatcag gcaaatcaaa acagaagtag
      361 tggatatttt cttgtctaaa aatccatcgc cgcatctcac aataccgaaa ggtacgatta
      421 ttacccgaca aaactattat tcaaaataca cctgtggaat gtttaccaaa aaatagtacc
      481 cccgaggcgc tcaaagttcg cattttggta aaaatttatc tagttgcaat gcaataacaa
      541 aacagaatag aactcaaaat tgctatttca ggagaactca aaatgagaaa ggttgatgct
      601 tttgaaagct cagatgagaa caatacttgc aatagtttat taggcataaa aataaaaaac
      661 attg
//
LOCUS       DAPGRN010000354          657 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPGRN010000354 DAPGRN010000000
VERSION     DAPGRN010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.361"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>657
                     /locus_tag="RHW33_004934"
     CDS             <1..>657
                     /locus_tag="RHW33_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDV1648331.1"
                     /translation="PLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVR
                     ENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHV
                     NDGFIFLGHRIIRKRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQL
                     NRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPG
                     Q"
BASE COUNT          180 a          155 c          182 g          140 t
ORIGIN      
        1 ccgctattat cgaacatcat gctgaatgag ttcgatcaat acctgcatga gcgctacctg
       61 agcgggaaag ccagaaaaga tcggtggtac tggaataaca gtatccaacg gggccgaagt
      121 acggcggtca gagaaaactg gcagtggaaa cccgcggtgg cgtactgccg ctatgccgat
      181 gattttgtcc tcatcgtcaa aggcaccaaa gcacaggcgg aagccatcag ggaggagtgt
      241 cggggtgtgc tcgaaggcag tctgaaactc aggctgaaca tggataagac taaaatcacc
      301 catgttaatg acggctttat ctttctgggg cacaggatca ttcgcaaacg cagtcgttat
      361 ggcgagatgc gagtggtctc aacgatcccg caggagaaag ccagaaactt cgccgcatcg
      421 ctgacagcac tgttatcagg caactacagt gaaagcaaag tcgatatggc tgaacaactc
      481 aaccgaaaac tgaaaggctg ggccatgttc tatcagttcg ttgattttaa ggccaaagtc
      541 ttcagttata tcgaccgtgt cgtgttctgg aagctggctc actggctggc ccgcaaatac
      601 cgtacaggta tcgcttccct gatgaggtgg tggtgtaaat caccgaaacc gggtcag
//
LOCUS       DAPGRN010000355          623 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPGRN010000355 DAPGRN010000000
VERSION     DAPGRN010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.362"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(84..431)
                     /gene="trbA"
                     /locus_tag="RHW33_004935"
     CDS             complement(84..431)
                     /gene="trbA"
                     /locus_tag="RHW33_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HDV1648332.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(447..>623)
                     /gene="traF"
                     /locus_tag="RHW33_004936"
     CDS             complement(447..>623)
                     /gene="traF"
                     /locus_tag="RHW33_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HDV1648333.1"
                     /translation="DSRTDQGQAQRLGVKYFPAMMLVDPKQGSVRPLSYGFISQDDLA
                     KQFLNVSEDFKPNF"
BASE COUNT          200 a          150 c          118 g          155 t
ORIGIN      
        1 ctggattgcg gtatggttta tcatatggtc ttcagtaatc aacctcatta ttttgtatgg
       61 tttttatgac cgttttatat gacttagcct gtcataagcc cccttataaa aaaccacatc
      121 actccggacc ataccgatga caacacaata cagataaata ttcttctgaa cctgtcgaag
      181 ctcgtaacgt ccggtaatgc ggccgcaact attgaaacaa ccagtgctgc gccctgactc
      241 ataattgcac cacacagaaa ccagagactg gcggtaagca catataaaca cgcgatatta
      301 ataacaagga aaaccagtga gctcaggaga aacattttcc tgtcattacg ctcagaaaag
      361 aaataaccgg caataatgac aaatattaac agaacaacac ctgtaaacat tttcaaataa
      421 tcttcactca taaatcaaat ccgcgattaa aaattgggtt taaaatcttc agaaacgttc
      481 aggaactgtt ttgccaggtc gtcctgcgaa ataaagccgt atgataacgg gcgaacactg
      541 ccctgtttcg ggtcaaccag catcatggcc gggaaatatt tcacgccgag gcgctgcgcc
      601 tgcccctggt cagtccggga atc
//
LOCUS       DAPGRN010000356          622 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPGRN010000356 DAPGRN010000000
VERSION     DAPGRN010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.363"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..404)
                     /locus_tag="RHW33_004937"
     CDS             complement(<1..404)
                     /locus_tag="RHW33_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648334.1"
                     /translation="MNKNLLTRVIIISDDLYYLQGLSAKLGHHYRFFIECFFCSQNAC
                     NNDLSTLRFDNRFTKNVLLVAVDDVSILAKLPDLARFSMIVSLRKMKNRNIFFSLSEQ
                     TFISRYIKLNKLITLIKSNDKDQQVRQVTLTR"
BASE COUNT          206 a          110 c          105 g          201 t
ORIGIN      
        1 taccttgtca gagtcacctg cctgacttgc tggtctttat cattactttt aatcaacgta
       61 atgagtttat tcaattttat atatcggcta atgaatgttt gctctgaaag tgaaaagaag
      121 atatttctgt ttttcatctt tcttagtgaa acaatcattg aaaacctggc taaatctggc
      181 agttttgcaa gtatacttac atcatccact gcgacaagaa gtacgttttt ggtaaagcgg
      241 ttatcaaatc taagtgtact taggtcatta ttacaagcat tttgagaaca aaagaaacac
      301 tcaataaaaa aacgataatg gtgaccaagt tttgcactta atccctggag gtaatataga
      361 tcatccgaaa tgataattac ccttgttagt aagttcttgt tcattgcact aactcaattg
      421 cttgcagcaa aacagcccat cttcatgcag taaaaatttt gaaagtgaat aaagcttttt
      481 actaaagatg taacaatggg cattccatat gcgacataac aactgcagta acacaactaa
      541 tctttgcgtg ttctttatat tagagagtaa cagcgtgata tctgtatctc taaaagaata
      601 gcgatgccct gcaatcgatg ag
//
LOCUS       DAPGRN010000357          621 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPGRN010000357 DAPGRN010000000
VERSION     DAPGRN010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.364"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..219
                     /gene="tnpB"
                     /locus_tag="RHW33_004938"
     CDS             <1..219
                     /gene="tnpB"
                     /locus_tag="RHW33_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV1648335.1"
                     /translation="IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSI
                     TRSQLAMLLDKLDWRQPKTSSRNSLTML"
     gene            250..>621
                     /locus_tag="RHW33_004939"
     CDS             250..>621
                     /locus_tag="RHW33_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV1648336.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRFAL
                     TRAEEKIEEDEREIEHLRAQIEKLSRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLP"
BASE COUNT          163 a          159 c          170 g          129 t
ORIGIN      
        1 atcttccgtg gccgacgggg tgacaccgtc aaaattcttt gggctgatgc tgatggtctg
       61 tgcctgttca ccaaacgcct ggaggaaggc cagtttatct ggcctgcggt acgtgacggc
      121 aaggtatcca ttacccgctc gcaactggca atgctcctcg ataagctgga ctggcgtcag
      181 ccaaaaacat ccagccgtaa ctcactgaca atgttgtaaa aaactcctga cggcattata
      241 aaaacggtca tgagtcagaa atacctcatt cgcatcgcag agctggaaag gttgctctct
      301 gagcaggctg aagccctccg tcagaaagac cagcaactga gtctggttga agagacggaa
      361 gccttcctgc gctttgcact gacacgtgcc gaagaaaaga tcgaagaaga tgaacgggaa
      421 atagaacatc tgcgggctca gatagaaaaa ctgagccgga tgctgttcgg tacccgttct
      481 gaaaaactgc gtcgtgaagt tgaactggct gaggctctgc tgaaacaacg tgaacaggac
      541 agcgatcgtt acagtgggcg ggaagacgat cctcaggttc cccgccagtt gcgacagtcg
      601 cgccatcgtc gtccgttacc g
//
LOCUS       DAPGRN010000358          611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPGRN010000358 DAPGRN010000000
VERSION     DAPGRN010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.365"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>611
                     /gene="tuf"
                     /locus_tag="RHW33_004940"
     CDS             <1..>611
                     /gene="tuf"
                     /locus_tag="RHW33_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV1648337.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRG"
BASE COUNT          135 a          149 c          184 g          143 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcctacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg g
//
LOCUS       DAPGRN010000359          607 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPGRN010000359 DAPGRN010000000
VERSION     DAPGRN010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.366"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..181
                     /locus_tag="RHW33_004941"
     CDS             <1..181
                     /locus_tag="RHW33_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDV1648338.1"
                     /translation="SLAESIRQEISTFSGMNRIYLTGGGAELIYPHIKQYFPNLKVNK
                     VDEPQFALVKAMVHA"
     gene            181..576
                     /locus_tag="RHW33_004942"
     CDS             181..576
                     /locus_tag="RHW33_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HDV1648339.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSASINPKDDFEEQTRRNLSMLKK"
BASE COUNT          219 a          132 c          120 g          136 t
ORIGIN      
        1 atctctggca gaaagcatca ggcaggaaat ttcaacattc tcaggaatga accgaatcta
       61 cctcacaggc gggggagccg agcttattta ccctcacata aagcaatact tcccgaatct
      121 gaaagtgaac aaggtagatg aacctcagtt tgcattggta aaagcaatgg tgcatgcgta
      181 atggaaagca gtgatccaaa aaaaagaaag aaagttgtgg catatctgca cccggcactt
      241 tatccgcagg acaacctgac acaacaaaca atagactctc tcccagtaca aatgcgggga
      301 gatttctaca gacaatccct tatttgcgga gcagctcttt attctgttga cccccgactg
      361 ttaacactaa tcagcggttt tttcagtgag aaaatcactg cagaaaatct ggtgaaactt
      421 atagaacaaa caacaggata cacatcgaca tcaattgata taagtgtgtt aaaaaacatc
      481 atagaggcat cttcagaaaa caaatcagcg agcatcaatc cgaaagatga tttcgaagaa
      541 caaaccagac gcaatctgtc tatgttaaaa aaatgacaaa gaggggcaga atatattctg
      601 cccactc
//
LOCUS       DAPGRN010000360          602 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPGRN010000360 DAPGRN010000000
VERSION     DAPGRN010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.367"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..265)
                     /locus_tag="RHW33_004943"
     CDS             complement(<1..265)
                     /locus_tag="RHW33_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV1648340.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSVQTLIKQLLVMKSAE
                     ENAFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFNGKKINR"
     gene            complement(308..544)
                     /locus_tag="RHW33_004944"
     CDS             complement(308..544)
                     /locus_tag="RHW33_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV1648341.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          164 a          132 c          102 g          204 t
ORIGIN      
        1 ttctgtttat tttcttacca ttgaacatac ttttattgtc agctatcgct gttcttcggt
       61 ttggccctcc tgtatcactg ttgctaaaat cacgaattat gctgtcaatt aaatctcgtc
      121 ggaaagcatt ttcctcagcc gatttcatta cgagtaattg cttaataaga gtctgcaccg
      181 aaaaatttat cttcatgcgc agtatctgga gcaccgcctg gccagtaatt tcatatttta
      241 tactttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa ataatcaggc
      301 tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt tcctcttccc
      361 ctgtcgcttg ttccagtcgg caaagaagtt tggcacaaag cgactttcga ctcagattac
      421 gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga gtaggcaagt
      481 tagcttttgc aaagtaacga gtgattgctc ccgcagaatc taaagtcact gaattttgat
      541 gcatgctacg cctctgttaa aaatgttaat caatgttaag tgaggtaaca atagcaaact
      601 tg
//
LOCUS       DAPGRN010000361          595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPGRN010000361 DAPGRN010000000
VERSION     DAPGRN010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.368"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>595)
                     /locus_tag="RHW33_004945"
     CDS             complement(<1..>595)
                     /locus_tag="RHW33_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV1648342.1"
                     /translation="YSRRYAINENIAKDAVTNNIALMSTFYDSDNTSGEVLNNDFTEE
                     SFETALDVEHTIVLGFADDNFETKPVHYQDLLIRFSAFQDTVFNLFPEMRSSHYNDIC
                     SVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLNDGCFIKYSH
                     FNDVALIKQNINNLHLSHTVVNESILKKCCFENGSLG"
BASE COUNT          192 a          119 c           86 g          198 t
ORIGIN      
        1 tcacctaaac tgccgttttc aaagcaacac ttcttcaata tactttcatt cacaacagta
       61 tgtgataaat ggagattgtt tatgttttgc tttattaatg ccacgtcatt aaaatgacta
      121 tatttaataa agcaaccatc attgagtagt gttttaagat tgactaaatt acttgcatct
      181 tcacgtacca tcgtggcaac tggaacaggc agagacatga aaacttcttc attcaccatc
      241 aagtgaatca tgcattgcgt tccctttgtc atatccacac ttacgctaca aatatcgttg
      301 taatgcgaag agcgcatttc agggaacaga ttaaacacgg tatcttgaaa ggcactaaat
      361 cgtattaata aatcctgata atgaacaggt tttgtttcaa aattatcatc agcaaatcca
      421 agcactattg tatgctctac atctaacgca gtttcaaatg actcttctgt aaaatcatta
      481 tttaaaacct cacctgacgt attatcactg tcataaaaag tgctcatcaa tgcgatattg
      541 tttgttacag cgtcttttgc aatattttca ttaattgcat atcgcctgct ataat
//
LOCUS       DAPGRN010000362          591 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPGRN010000362 DAPGRN010000000
VERSION     DAPGRN010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.369"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>591
                     /locus_tag="RHW33_004946"
     CDS             <1..>591
                     /locus_tag="RHW33_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648343.1"
                     /translation="LERGYAWYSEKIAKHTNSRYSHAMIYVGGTIIEATRGGGVYSRI
                     PNRSTVRDISDFKVLRLKEFPGKDILQVICDHARSLCGSQYSVSQALKVKGPDFLRKF
                     ANDSRKQFCSRLVAQCYQKAGIQLTENINFCSPGDIERSDYLIEQPEMVYRASEEDVA
                     HAQGESPHTQHTKNAVNFVRNALSIFENYGIKTVGSP"
BASE COUNT          188 a          101 c          144 g          158 t
ORIGIN      
        1 tttggagaga ggttatgctt ggtattcaga aaaaatagct aaacacacca atagtcgtta
       61 ctctcatgca atgatatacg taggtgggac aataattgag gcaactaggg gaggtggggt
      121 gtatagccga attccaaacc gttctactgt acgtgatatt agcgacttta aggtactaag
      181 gcttaaagag tttccgggaa aggatatctt acaagtaata tgtgaccatg ctcgtagtct
      241 ctgtggtagc cagtattcag taagccaggc tctcaaggtt aaagggccgg attttttaag
      301 aaaatttgcc aacgacagta gaaaacaatt ttgttcacgg cttgtggctc aatgttacca
      361 gaaggctgga atacaactga cagagaatat aaatttctgt tcaccagggg atattgagag
      421 aagcgattac ttaatcgaac agcctgaaat ggtctatcgt gcatcggaag aagatgttgc
      481 acacgcgcag ggggagtcgc cgcatacaca acatacaaaa aatgcagtaa attttgttag
      541 aaatgcattg agtattttcg aaaattatgg gattaagact gtcggctctc c
//
LOCUS       DAPGRN010000363          581 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPGRN010000363 DAPGRN010000000
VERSION     DAPGRN010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.370"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            277..>581
                     /locus_tag="RHW33_004947"
     CDS             277..>581
                     /locus_tag="RHW33_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDV1648344.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     "
BASE COUNT          211 a          129 c          123 g          118 t
ORIGIN      
        1 ggcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctggtt
       61 aagtagtttc cgtctcagtt tgtgacgcaa aatgagaaac cagcaacaac tcaaattgag
      121 ttacagcaag aacacaaaga gactctaaaa gagtcacatt acttgacatt aacccaaaat
      181 gagacacaat aagaaataac aaaaactcaa aaagatgcat cgacataaaa gatgctcaat
      241 ttgagacaaa cacaaactca aaacgaggca aaaagaatgc taaaagtatc atgcgatgat
      301 ggttcaacta acgtaaaact ggcctggctt gaagatggcg aggtaagaac cagtctgtca
      361 ggaaacagct ttaaagaagg ctggaacccg ggactgttta atgccggcaa agtttacaat
      421 tacgtcgtgg atggaaaaaa atacacctat gatttgggca gcactgctgt cataggtaca
      481 acacatgtca gttatcaata cagcaccacc aatctgcttg ccatacatca tgcactgctg
      541 acatccggcc ttcagccaca ggatgtagaa ctgacagtaa c
//
LOCUS       DAPGRN010000364          580 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPGRN010000364 DAPGRN010000000
VERSION     DAPGRN010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.371"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..475
                     /locus_tag="RHW33_004948"
     CDS             <1..475
                     /locus_tag="RHW33_004948"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648345.1"
                     /translation="TIGFFSLSAIAATGPSINSKTLSITLHSPANITANWVAESDLTP
                     AKIMQPETLVGVISLSNTSIETATIRSTKPADASQQGVITFENGSGDTFGTTISTNNS
                     NVSIEKRTGESVISSTTQGAFLPSNLDLNVKTTSGNTTVSSGQYNAVVSITTQIK"
BASE COUNT          199 a          112 c          100 g          169 t
ORIGIN      
        1 aacaattgga ttcttttcct taagtgctat tgcagctaca ggaccttcaa ttaattcaaa
       61 aacattaagt ataactcttc actctccagc caatattact gcaaactggg ttgcagagtc
      121 agatttaact cctgctaaaa taatgcagcc agaaacgttg gttggtgtta ttagcctatc
      181 caataccagt attgaaacag caactattcg aagcaccaag cctgctgatg ctagccagca
      241 aggtgttatt acttttgaga atggtagcgg tgacacattt ggcactacca tatcaacaaa
      301 taactccaat gtatccattg aaaagagaac tggagaaagt gttattagct caaccactca
      361 gggcgcattt ttaccatcaa atttagatct taatgtaaaa acaacatctg gcaatacaac
      421 tgtatcgtct ggacaatata atgctgtagt atcaattacc acgcagatta aataatcttc
      481 tcaagcctcc ttttttagag gaggctaaac tccatagtgg cgatagaaag ttgagatgat
      541 aatgaaaaat aaaaaactaa ttatattttt gtatggtgca
//
LOCUS       DAPGRN010000365          577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPGRN010000365 DAPGRN010000000
VERSION     DAPGRN010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.372"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..437
                     /locus_tag="RHW33_004949"
     CDS             <1..437
                     /locus_tag="RHW33_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648346.1"
                     /translation="ELLRQSNGNITNDKIDEIIACLASPQGITCFYYSSAQGYQSRLQ
                     HTSQDVRELLSKSLDGATSSTFHLKKSGNAFFGSGKYLNEMDFTMNGFSQKIYLMAVN
                     KIENEVKLQCARYFTTGRVDEQNSISDKIAFTFFGAATTADL"
BASE COUNT          186 a           88 c          115 g          188 t
ORIGIN      
        1 gagaattatt acggcaatca aacggtaata ttacgaatga taagattgat gaaataatag
       61 cttgcttagc cagtcctcag gggattacat gtttttatta ttcttccgca caaggctatc
      121 agagtcgctt acaacacaca tctcaagatg ttcgcgagtt actctcaaag agtttggatg
      181 gtgccacttc atcaacattt catttaaaaa aatcaggaaa tgcatttttt gggtctggaa
      241 agtatcttaa tgaaatggat ttcactatga atgggtttag tcagaaaata tatttgatgg
      301 cggttaataa aattgaaaat gaagttaagc ttcagtgtgc tcgttatttt actaccggta
      361 gggtcgacga acaaaactcc atcagcgata aaatcgcttt tacatttttc ggtgccgcaa
      421 caactgctga tttataattt tttcctgata aatcgcgttt atcattaagg tgaacgagta
      481 ttcttaaccg tctgtgtgta aacagtcttt ttgaaaaaaa atgagcgatt tttgatagtg
      541 aataagtaga gttagtgatg caattgcatt ggagaaa
//
LOCUS       DAPGRN010000366          567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPGRN010000366 DAPGRN010000000
VERSION     DAPGRN010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.373"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..>567
                     /locus_tag="RHW33_004950"
     CDS             58..>567
                     /locus_tag="RHW33_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV1648347.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPDVSATKLQTNGQVSGVQTDVPIELKDCDTTVTKNATFTFNGTADA
                     TQITAFANQASSDAATNVALQMYMNDGTTAIKPDTETGNILLQDGDQTLTFKVDYIAT
                     GKATSGNVNA"
BASE COUNT          171 a          104 c          136 g          156 t
ORIGIN      
        1 aagtctaatg tcatttaaag gcattatcat atttacgaag agatcaggat gataacgatg
       61 aaaaaaagtg tattgacggc gtttataact gtggtatgtg caacgtccag tgttatggca
      121 gctgatgata atgctatcac ggatggttca gtaacattta atggtaaagt tattgctcca
      181 gcttgtaccc tggtagctgc gacgaaagat tccgtggtga ctttgccaga tgttagtgcc
      241 acgaagttgc aaaccaatgg tcaggtttct ggcgtgcaaa ctgatgtgcc aattgaatta
      301 aaagattgtg atactaccgt aacaaaaaat gcaacgttca cctttaatgg cactgcggat
      361 gctactcaga ttacagcgtt tgctaaccag gcctcatctg atgctgctac aaacgtggcc
      421 ctgcaaatgt atatgaatga tggtacaacg gccatcaagc cagacacaga aaccgggaac
      481 attttgttgc aagatggaga tcagacgttg acttttaaag ttgattatat cgctacgggg
      541 aaagcgactt ctggtaatgt gaatgcg
//
LOCUS       DAPGRN010000367          561 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPGRN010000367 DAPGRN010000000
VERSION     DAPGRN010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.374"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>561)
                     /gene="cpsB"
                     /locus_tag="RHW33_004951"
     CDS             complement(<1..>561)
                     /gene="cpsB"
                     /locus_tag="RHW33_004951"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: protein motif:HMM:NF012695.1"
                     /note="capsular polysaccharide colanic acid biosynthesis
                     protein; catalyzes the formation of GDP-mannose from GTP
                     and alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDV1648348.1"
                     /translation="SRLWPLSRNDYPKQFLRLCSPITMLQETINRLEGLDCEDPIIIC
                     NQKHKYIVKEQLLSFKIKHSAILLEPVGRNTAPAIGLAAFEAMGQKGDVLLLVLSADH
                     LIKNIECFHKAVNKSLKFALDNYLVTFGVTPTRAETCYGYIRKGALIDRSAYQISSFV
                     EKPDQDKAESFFKSDDYLWNSGMFLF"
BASE COUNT          176 a          115 c           92 g          178 t
ORIGIN      
        1 taaataaaaa catcccacta ttccataaat aatcatcaga cttaaaaaaa ctctcagctt
       61 tatcttggtc aggtttttca acaaaacttg atatctgata agcacttcta tcaattagtg
      121 cccctttgcg gatgtaccca taacaagttt ctgctcgcgt aggtgtcaca ccaaatgtta
      181 ctagatagtt atcgagtgca aacttcagtg atttgtttac agctttatgg aagcattcaa
      241 tattttttat caaatgatca gcagataata ccaagagaag cacatcgcct ttttgtccca
      301 tggcttcgaa ggctgccagt ccaattgctg gcgctgtatt cctacccacc ggctcaagaa
      361 gtatagcaga atgtttaatt ttaaaggata ataactgctc ttttactata tatttatgtt
      421 tctgattaca aataatgata ggatcttcac aatctaaacc ttctaatcta tttatggttt
      481 cctgaagcat cgtaataggg ctacagagcc gcaaaaactg cttgggataa tcgttacgcg
      541 ataatggcca taatctggaa c
//
LOCUS       DAPGRN010000368          556 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPGRN010000368 DAPGRN010000000
VERSION     DAPGRN010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.375"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..408)
                     /locus_tag="RHW33_004952"
     CDS             complement(<1..408)
                     /locus_tag="RHW33_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV1648349.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNGSEDNEEIIDDDNI"
     gene            complement(398..>556)
                     /locus_tag="RHW33_004953"
     CDS             complement(398..>556)
                     /locus_tag="RHW33_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDV1648350.1"
                     /translation="CAKRIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSW
                     LSRNNNVK"
BASE COUNT          186 a          123 c           72 g          175 t
ORIGIN      
        1 aatattatca tcatcgatta tctcttcatt gtcttcagaa ccattcacct tctgaatctt
       61 ttcaaaaaag ctaaccaact ccggttcaga atattttatc aaatatgatt tataaccact
      121 catgcctgtc tcaacaaaat taacaaccga cgctacatta ttgaatgaaa gattctcatt
      181 ctgactgtta ttatatattt ccttcccaac cggcatcatt acgaacattg acaaaagcaa
      241 agcaacccca ttaagtgtca tatttgatgg cacttgctga agtcccagcg cgtttcggac
      301 aataacaaaa acaatcgaga atttaataaa acatgtcccg gatgctatta taaacggcaa
      361 tagtgtaaat aatgataata tcgctatcag cgaaatgcta tttgacatta ttattccttg
      421 ataaccaaga atgaatttct acgcccaatt tatcatccac ttcaacaagt tcgccatagc
      481 cagttagcgc gccatttaca cgtatctcta tatttttctc agattcagga agcagagata
      541 ttattctttt tgcaca
//
LOCUS       DAPGRN010000369          549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPGRN010000369 DAPGRN010000000
VERSION     DAPGRN010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.376"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..121
                     /locus_tag="RHW33_004954"
     CDS             <1..121
                     /locus_tag="RHW33_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HDV1648351.1"
                     /translation="DQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            118..399
                     /locus_tag="RHW33_004955"
     CDS             118..399
                     /locus_tag="RHW33_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV1648352.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLTAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
BASE COUNT          148 a          106 c          160 g          135 t
ORIGIN      
        1 tgaccaggtt gaggcaggac gtcagcaact cgaacgcggc gatgtgcttc ctcatgacat
       61 ggtcgaatcc tccgctgctg catggcggga tgaaatgagc aggaaggtcg ccggtaaatg
      121 atggagatat tctggaccat gctggccagc caggacagga agcgcattcg tgagtacatt
      181 gcggagcaga acctgacggc cgccattgaa ctggacgaac ggattggtta ttcagccagc
      241 agcctggccg ggcagccgta taaaggtcgc aatggtcgtg tggaaggtac ccgggaactg
      301 gttattcacc cgcattttgt tctggtttat gaggtcgata gccagtgggg aaaagtgtat
      361 attctgcgag tgttgcatac agcacagaag tggccataaa gaagagtata tttttacctt
      421 actgggggct gattatctgt tgttactgtt gtggcataca tagaaaaaca gttgggtagt
      481 taaaatagca taaaggtttg tgaaatgatt ggaaaatcta tatctcgttt atgccaaaat
      541 gaacgcaaa
//
LOCUS       DAPGRN010000370          547 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPGRN010000370 DAPGRN010000000
VERSION     DAPGRN010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.377"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>547
                     /locus_tag="RHW33_004956"
     rRNA            <1..>547
                     /locus_tag="RHW33_004956"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          126 a          120 c          184 g          117 t
ORIGIN      
        1 acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa cggaggagca
       61 cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat aagccagctt
      121 gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc cggtggttct
      181 gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag gctgataccg
      241 cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat cacatcctgg
      301 ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg cgagctgggt
      361 ttagaacgtc gtgagacagt tcggtcccta tctgccgtgg gcgctggaga actgaggggg
      421 gctgctccta gtacgagagg accggagtgg acgcatcact ggtgttcggg ttgtcatgcc
      481 aatggcactg cccggtagct aaatgcggaa gagataagtg ctgaaagcat ctaagcacga
      541 aacttgc
//
LOCUS       DAPGRN010000371          543 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPGRN010000371 DAPGRN010000000
VERSION     DAPGRN010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.378"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>543
                     /locus_tag="RHW33_004957"
     CDS             <1..>543
                     /locus_tag="RHW33_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV1648353.1"
                     /translation="MSNSQYANFEPQLNIDAWRIKNQFVWSKEDNHNRLTSNYLYAER
                     GLNSIKSRIYIGSGYFPLRGFDSFKFQGGVLKTDESMYPSIEKKFSPVVKGIAKSQSK
                     VEIYQDKTMIYTTTVPAGPFEITDYLLSGSNSDLYVKVIDTYGNTQTFIVPYSLPAIA
                     VRNGYRYYEIAAGNYQELDE"
BASE COUNT          194 a           84 c          104 g          161 t
ORIGIN      
        1 aaatgagcaa ctcgcaatat gctaattttg aaccccaatt aaacatcgat gcctggcgaa
       61 taaagaatca gttcgtatgg tcaaaagagg ataatcacaa cagattaaca agtaattacc
      121 tgtatgctga gagaggtcta aatagcatca aaagccgtat ttatattggt tctggatact
      181 ttcctttaag aggatttgat tcgttcaagt ttcaaggtgg agtattgaag acagacgaat
      241 caatgtatcc gtcaattgag aaaaaatttt ctcctgttgt aaaaggaata gcaaaaagtc
      301 agtctaaggt ggaaatttat caagataaaa caatgatata tacaacgaca gtgcccgctg
      361 gtccttttga aataactgat tatttgttat caggctcaaa ttctgactta tatgtcaaag
      421 ttattgatac ttatggaaat acacaaactt tcatagtgcc ttattctcta ccggcaatag
      481 cagttaggaa tggatataga tactatgaaa ttgctgcagg taattaccaa gagttagatg
      541 aag
//
LOCUS       DAPGRN010000372          538 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPGRN010000372 DAPGRN010000000
VERSION     DAPGRN010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.379"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..>538
                     /locus_tag="RHW33_004958"
     CDS             44..>538
                     /locus_tag="RHW33_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDV1648354.1"
                     /translation="MLPLVKVSLPESNILMPSLEKVLYSGMIAEGDSVYTFERLFSEK
                     FGLQNGIAMSSGTAALHTALVLSEVGISDEVITTSMTAEPTNTTILQTGAKPIFADVD
                     VENGNILVSSIEEKITEKTKAILVVHYAGYPVDIKRIRKLADKHNLLLIEDCAHALGA
                     TFENK"
BASE COUNT          187 a           82 c          106 g          163 t
ORIGIN      
        1 catattcgtg ctgatattag taattataac caggatgtcg aaaatgctac cactagtaaa
       61 agtttcattg ccagaatcca atatattaat gccttcactc gaaaaagttc tttatagtgg
      121 tatgattgct gaaggagata gcgtatatac tttcgaaagg ctattttctg aaaagtttgg
      181 tttacaaaat ggaattgcaa tgagcagtgg aactgcagct ttgcatacag cacttgttct
      241 ttctgaggtt ggtatctctg acgaagtcat caccacaagt atgaccgctg agccaactaa
      301 tacgactatt ttgcaaactg gagcaaagcc tatttttgcg gatgtcgacg ttgagaatgg
      361 taatatatta gttagttcta tagaggaaaa aattacagaa aaaacaaaag caatattagt
      421 agtacattat gcaggttatc ctgttgatat aaagagaatc agaaaactag cagataaaca
      481 taatctgtta ctgatagaag attgtgctca tgctttagga gctacatttg aaaataaa
//
LOCUS       DAPGRN010000373          538 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPGRN010000373 DAPGRN010000000
VERSION     DAPGRN010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.380"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..71)
                     /locus_tag="RHW33_004959"
     CDS             complement(<1..71)
                     /locus_tag="RHW33_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV1648355.1"
                     /translation="MASVSISCPSCSATDGVVRNGKST"
BASE COUNT          191 a          129 c           88 g          130 t
ORIGIN      
        1 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
       61 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc
      121 atcatccgga aaatacaatc tccgttgacc gaaggagatc agtgaaggta atttaaaaac
      181 ggttctgtga aagtgacacg aaccgatctc catcgatgtt actcaccgat ctccttcacg
      241 gtaatacgca aaaatgacaa caaacagtgc ataaatattg aatacattta ctgagcatgc
      301 aaaagaaaaa tacaaactaa tcccggcctt tcacagaatt tcctctatca gaggctgagt
      361 tggcaatctg ctcacccagg aaatagtaac acacaaaaac ataaagagac tctaatgaac
      421 aagcgcaata catattcttc atctttcata gcatctcact tctttaacaa gaattattat
      481 tatctacgcc tcctcctttc atatatccct catgaatgat taagataaac acactgtg
//
LOCUS       DAPGRN010000374          537 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPGRN010000374 DAPGRN010000000
VERSION     DAPGRN010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.381"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            143..>537
                     /locus_tag="RHW33_004960"
     CDS             143..>537
                     /locus_tag="RHW33_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed virulence protein BigA"
                     /protein_id="HDV1648356.1"
                     /translation="MQRKKLLSVCVAMALSSQTWAADTSIPDSTEKTRKSSKITCPAN
                     IHSLSKEQLENLSAECRENKDSAVLPWAAAGLAAVATGIAIYTLSDDDNHHHNNSPVP
                     DDGGDTPVPPDDGGDTPVPPDDGGDTPVPP"
BASE COUNT          152 a          113 c          117 g          155 t
ORIGIN      
        1 ttttacttgc ctgattttaa tattgatttt gtgaattata taagcatttc tttattgctt
       61 atttcactat gaatatttac tacgatttat cttcaaattc tgcatggctt gttacgcacg
      121 gtctagatct aaggaatttt atatgcaaag gaaaaaatta ttgtctgttt gtgttgccat
      181 ggcgttaagt agccagacat gggctgcgga tacttcaata cccgactcaa ccgaaaaaac
      241 acgtaagtcc agtaagataa catgtccggc aaatattcat tccctgagca aagagcagct
      301 agaaaattta tccgcggagt gcagagaaaa taaagatagt gcagttttac catgggcagc
      361 tgcaggtctt gctgcagtag ctacgggaat tgctatatat accttaagtg acgatgacaa
      421 tcaccaccat aataactccc cagttccgga tgatggcggt gatacgcctg ttccgccaga
      481 cgacggcggt gatacgccgg ttccgccaga tgacggcggt gatacgccag ttccgcc
//
LOCUS       DAPGRN010000375          531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPGRN010000375 DAPGRN010000000
VERSION     DAPGRN010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.382"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            33..531
                     /locus_tag="RHW33_004961"
                     /pseudo
     CDS             33..531
                     /locus_tag="RHW33_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313341.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          179 a          105 c          105 g          142 t
ORIGIN      
        1 agagtgtgga attttaagaa aggacatctg aaatgttaaa ccgtgacttc gatcatctct
       61 caacgctatt acatctcttt tttgaacgtg aggatatttg ccaaaagctt aatattattg
      121 accaaaacaa tatcaccggt tgggaagtgt ggttccaggt agagtttgct aatatgctct
      181 gctcaacaga ccatgaatgg tggcgtgaac aagcactttc ctgtgatatg cgcaaaaaac
      241 ctgagcgccc gacactacgc acagatttct tactacgtaa aaaaggctgg gcacaagata
      301 gctatatcac gcttgaaata aaacaaaaca gagacgcgac cagttgtgtt aaaaatatga
      361 tcgcagactt agaaaaaagc gcaaaaataa aacgttccga actcgattta cgcagtttct
      421 gaaccttggg tatcacccct atgatagaca gggaaaggct ggactctctc atcgattgct
      481 atttagatga gaaattttat cttactaaat ctcgtaaaaa acacgtttta c
//
LOCUS       DAPGRN010000376          515 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPGRN010000376 DAPGRN010000000
VERSION     DAPGRN010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.383"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>515
                     /locus_tag="RHW33_004962"
     CDS             <1..>515
                     /locus_tag="RHW33_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648357.1"
                     /translation="LDNLRGDLDQATISSSQSSSSAEQSSIKISKYNILDMLDNLTMR
                     QQAVMEEFLRVFTSMYKASAMSEAIMSNVFEQLVQYQKTETVHATMASSAGVLAGSTV
                     GAAGAAASVVANFRSHPEKMQTLTPDQKHTISGTDDKTQRGLKREVQDQQAYLKQIDE
                     EIKTTTKDTTL"
BASE COUNT          183 a          103 c          104 g          125 t
ORIGIN      
        1 cgctagataa tttgaggggt gaccttgacc aagctacaat ttcatccagt caatcaagca
       61 gttctgcaga acagagttct attaaaatat ctaaatataa tatccttgat atgctagata
      121 atttgaccat gcgacaacag gctgttatgg aggaattttt aagggtattc acatcaatgt
      181 ataaagcctc tgctatgtct gaggctatta tgagcaacgt ttttgaacaa ctagtccagt
      241 atcagaaaac agaaaccgta cacgccacaa tggcaagttc ggctggtgtt cttgccgggt
      301 caacagttgg cgcagctgga gctgcagcat ctgttgtagc aaatttcaga agccatcccg
      361 aaaagatgca aacccttaca cctgatcaga agcacactat cagtggaact gatgataaaa
      421 cacagcgagg attaaaaagg gaagtacaag atcaacaagc ttatctcaag caaatagatg
      481 aagaaataaa aacaacaacg aaagatacaa ctctt
//
LOCUS       DAPGRN010000377          510 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPGRN010000377 DAPGRN010000000
VERSION     DAPGRN010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.384"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>510
                     /locus_tag="RHW33_004963"
     CDS             <1..>510
                     /locus_tag="RHW33_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648358.1"
                     /translation="KEIYNGDPDFIFDKISRNLIHTVTKSDENGISKPTDLFTWHGKD
                     KKYNTLAIVIKNTNGNDYLSLGYYDQDNYHIKRGIRINGDSLTQYCSENARNASASFE
                     SNKALMAELFATGINHQVVNELNGERLKEPNEVFKRYGRAIRYNSQVDNAKYRRNNVK
                     EIVSTLFDN"
BASE COUNT          204 a           87 c           89 g          130 t
ORIGIN      
        1 taaaagaaat atataatgga gatcctgatt tcattttcga taaaatatca cgtaacctca
       61 tacatacggt aacgaaatcc gatgagaacg ggataagcaa accaacggac ttatttacct
      121 ggcacggtaa agataaaaaa tacaacactc tcgccattgt aattaaaaat acaaacggaa
      181 acgattattt atctctcggt tactacgatc aggacaacta ccacattaaa agaggaattc
      241 gtattaatgg tgatagtctc acacaatatt gtagtgaaaa cgccaggaat gcttcagcgt
      301 catttgaaag caataaagct ctcatggcag aattatttgc aacaggtata aatcatcaag
      361 ttgtaaacga actcaacggg gaaagactga aagaaccaaa cgaagttttt aaacgttatg
      421 gtcgagcaat aagatataat tctcaagtgg acaatgcaaa atatagacgc aataatgtaa
      481 aagaaatagt ttctacttta ttcgataacg
//
LOCUS       DAPGRN010000378          503 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPGRN010000378 DAPGRN010000000
VERSION     DAPGRN010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.385"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            103..294
                     /locus_tag="RHW33_004964"
     CDS             103..294
                     /locus_tag="RHW33_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HDV1648359.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          131 a          118 c           92 g          162 t
ORIGIN      
        1 ccactgacgc ttttttacat aacgaagaat tgaccatttt gtcctgttgt gccttaatgt
       61 aagtaccgtc cacagcgtgg gacatacttc aaggaacctt ttgtgagtca ggcaaccagt
      121 atgcgaaaac gacaccgatt taacagtcgc atgacccgta tcgtactgct catcagcttt
      181 atcttcttct ttggccgttt tatctactcg tccgtcggtg cctggcagca ccatcagagc
      241 aaaaaagaag ctcagcaatc cacactctcc gtcgaatcac cggtacaacg ttagcggtta
      301 ccttctccac tttcacagaa cataacggca cttcgctgtc ggatgctttt gctctttggg
      361 attatcaaag cggcagatat tctttcatct taaattttac gtctttgtcc tgactgatgt
      421 ttatcctgtt tggctgcgaa ataaatataa aattaatata tatgttgtaa tgatatattt
      481 ttataaatta ttccctgcgt gaa
//
LOCUS       DAPGRN010000379          492 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPGRN010000379 DAPGRN010000000
VERSION     DAPGRN010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.386"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..360
                     /locus_tag="RHW33_004965"
     CDS             <1..360
                     /locus_tag="RHW33_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDV1648360.1"
                     /translation="LTNHTFFDDAVLTPEQYDALLTTWCVYDGSLLTTPIEDVDLSGY
                     TAHFCNGRLYLIAPNFTSEALKALLQKLDSDEDFAPNKVVFYGCNFESAKQRELNEAL
                     KSYANKKSIELDLVVRN"
BASE COUNT          149 a           85 c          106 g          152 t
ORIGIN      
        1 ctcactaatc atacattctt tgatgatgcg gtattaacgc ctgaacagta tgatgcccta
       61 ttgactacat ggtgtgtata tgatggtagc ttgttaacaa cacctattga agatgttgat
      121 cttagtggct atacagcaca cttttgcaat ggccgtctgt atttgattgc acctaatttt
      181 actagcgaag cattgaaagc gttgctccaa aagctggatt cagatgaaga ttttgcccca
      241 aacaaagtcg tgttttatgg ttgtaacttt gaaagtgcaa aacaaaggga gcttaatgag
      301 gcgctaaaaa gttatgctaa caaaaaatct attgagttag atttagtggt aaggaactaa
      361 tcatgtcaaa agggttcaca ttcgaaaaga acttaccgca tcagaaagca ggtgtcgatg
      421 cggtgatgaa cgtttttgtt tccgcgacat ctcatcagga ggataatgtt tctattcgcc
      481 tgttagttaa tc
//
LOCUS       DAPGRN010000380          490 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPGRN010000380 DAPGRN010000000
VERSION     DAPGRN010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.387"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            9..440
                     /locus_tag="RHW33_004966"
     CDS             9..440
                     /locus_tag="RHW33_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV1648361.1"
                     /translation="MKSKRRERHQEILSEQLKSDVSNSRLMIANPTHIAIGIYFKPHL
                     SPIPLISVRETNEVALAVRKYAKEIGIPIITDKKLARKIYATHRRYDYVSFENIDEIL
                     RLLLWLEDVENAGQPVPDEQFSSEDKFIEGEDTEIENKDNN"
BASE COUNT          178 a           82 c           92 g          138 t
ORIGIN      
        1 atccagaaat taagtctaaa cgcagagagc gccatcagga aattctttct gagcaattga
       61 agtctgatgt cagtaatagt cgtttgatga ttgccaaccc tactcacatt gcaataggga
      121 tatactttaa gccacatctt tcacctattc cattgatttc tgtaagagaa actaatgagg
      181 tagcattagc cgtaaggaaa tatgcaaagg aaatcgggat accaattatt acagataaaa
      241 aattagcacg aaaaatttat gctacccatc gtcgctacga ttatgtgagc ttcgaaaata
      301 tagatgaaat attacgtctt ctgctgtggc ttgaagatgt ggagaatgcc ggacaacctg
      361 ttccagatga acagttctct tcagaagata aattcattga gggtgaagac acagaaatcg
      421 agaataaaga taataattga ataaattaat ttttatcgta gtcagatatt taaaaataat
      481 ctacacgcaa
//
LOCUS       DAPGRN010000381          485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPGRN010000381 DAPGRN010000000
VERSION     DAPGRN010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.388"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..393
                     /locus_tag="RHW33_004967"
     CDS             <1..393
                     /locus_tag="RHW33_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDV1648362.1"
                     /translation="NWNLFPLICLLSGCHFYRERFAERGFFYKVPAVLRDYLSAIPVE
                     INEKARYKPGIANYHNIITCGFSTLLPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLA
                     STLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          161 a           80 c           87 g          157 t
ORIGIN      
        1 aattggaatt tatttccatt gatctgttta ctcagtggtt gtcactttta tcgagaaagg
       61 tttgcagaac gcggattttt ttataaggtt cctgctgtgc taagggatta tttatcagct
      121 ataccggtgg aaataaatga aaaggcacga tacaaacccg gcatcgctaa ctatcacaat
      181 atcattacat gtggtttttc aacgttgtta ccctatataa ggcaacaacc attggcaatg
      241 cagcaacgat ttaatttatt atttcctgat tttgttgacc acatacaatt gccgttacca
      301 cttgcgtcaa ctcttttaga gagaattaca ttttatgcga aaaaaaatag agatgagctt
      361 gataaaatct cctgcaaatg gtgttgtgat taaaagaaaa atttctgatg gattgaaaga
      421 aatagtttct cttaaagaaa atatccttct tgaaaccacc gaaaaaattc agagcattga
      481 ggtaa
//
LOCUS       DAPGRN010000382          484 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPGRN010000382 DAPGRN010000000
VERSION     DAPGRN010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.389"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..300)
                     /locus_tag="RHW33_004968"
     CDS             complement(<1..300)
                     /locus_tag="RHW33_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648363.1"
                     /translation="MTFNTSGLTDITIVSGRKAVDRQGGNNFAFFHNASGNYRLRSSL
                     VTTSNNVSLSDIGLQGVKIAPINGSPLLPDSFAFRMKVNTNNGGPNLPAGEYTSII"
BASE COUNT          160 a           98 c           89 g          137 t
ORIGIN      
        1 gatgattgat gtatactcac ctgcaggtag attcggtcca ccattattag tattgacctt
       61 cattctaaat gcaaaagaat cggggagcaa aggactaccg tttataggag ctattttaac
      121 tccctgaaga ccaatatcac ttaaagaaac gttgttacta gtagttacca aagaggaacg
      181 aagtcgatag ttccctgatg cgttatgaaa aaatgcaaaa ttattccctc cctgtctgtc
      241 gacagctttt cgtccagaaa caatggtaat atcagttaat ccagaggtat taaaagtcaa
      301 gattccagca tcagctgcta cagaaatacc atagttagtt cctgcgtata aattttgatt
      361 tggttcaaaa ctaacattga gcacaggtgt gcttgttact gatacatcaa gcctctgatt
      421 atttacacta ggaccagtag cagcattagc acccgacacc ataacgaata agagtcctat
      481 taaa
//
LOCUS       DAPGRN010000383          479 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.391, whole genome shotgun sequence.
ACCESSION   DAPGRN010000383 DAPGRN010000000
VERSION     DAPGRN010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.391"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            165..240
                     /locus_tag="RHW33_004969"
     tRNA            165..240
                     /locus_tag="RHW33_004969"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:198..200,aa:Ile,seq:cat)
BASE COUNT          128 a           86 c          125 g          140 t
ORIGIN      
        1 ttgttgactg cgagtatcga ctttgcgaat ggaggcgatg tcgggtttta tgtcgcatgt
       61 attgacgacc caggatggat gagaacgcat aaaaatctgt tatttacatc ataagatcat
      121 catgtttatt gcgtggttta taagtattgt tattgcatac ttaaggccct ttagctcagt
      181 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      241 aatcatcatt catgtatcag gaagagttaa gggtacaagg ggtactcgat ggcggtctat
      301 atttcatgac taacgccatt taaaggaaga agtgcggtgg gtggtttcga atgagggctg
      361 gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc attcaaaagt
      421 acctggaaaa aatgcccgct atgccaagcg ggcaaattgc ttacatgtgg aattaagta
//
LOCUS       DAPGRN010000384          465 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPGRN010000384 DAPGRN010000000
VERSION     DAPGRN010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.392"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..460
                     /gene="ssb"
                     /locus_tag="RHW33_004970"
     CDS             <1..460
                     /gene="ssb"
                     /locus_tag="RHW33_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDV1648364.1"
                     /translation="LIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDKQTGQQKEQTE
                     WHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEIIVSQGGTMQM
                     IGARRDDSQFSNGWGQSNQPQNHQQYSGGSKPQSNANSEPPMDFEDDIPF"
BASE COUNT          149 a          109 c          123 g           84 t
ORIGIN      
        1 cctgattggt accctggggc aagacccgga gatcaggtat ataccaaatg gcggagcggt
       61 cggaagactc agcatcgcaa cgaatgaatc atggcgcgac aagcaaacgg gccaacagaa
      121 agagcaaaca gaatggcata aagtcgtttt gttcggaaaa cttgctgaaa ttgcgagtga
      181 gtatttacga aaaggttctc aggtctacat cgaagggaaa cttaaaaccc gtaagtggac
      241 agatgacgcc ggtgtagaac gttacacgac ggaaattatc gtcagccagg gcggcaccat
      301 gcaaatgatc ggcgctcgcc gcgacgattc acaattctca aatggctggg ggcagtcaaa
      361 ccaacctcaa aaccaccagc aatacagcgg tggcagtaaa cctcagagca acgccaatag
      421 cgaacctcca atggactttg aagacgatat tccattttga gaggg
//
LOCUS       DAPGRN010000385          459 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPGRN010000385 DAPGRN010000000
VERSION     DAPGRN010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.393"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>459)
                     /locus_tag="RHW33_004971"
     CDS             complement(<1..>459)
                     /locus_tag="RHW33_004971"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648365.1"
                     /translation="QGGYYWWKDIDFNRKSYYEQAGAFWYPSATFEPRLIRADESLRA
                     LLSGGGLSVGLLPRLYHGIVRRFVLDNLKKKTGRYVHGASPDISLAVSLALLGVNTFY
                     ISTPLTIFGASKKSGGGWTAENKHYGSIREQQHIPQYTKDNWSSFLPDVWS"
BASE COUNT          145 a          114 c           76 g          124 t
ORIGIN      
        1 agaccataca tcaggcaaga aactagacca attatcctta gtatattgag gtatatgctg
       61 ctgttctctt atactaccat agtgcttgtt ttcggcagtc catccaccac cacttttctt
      121 tgaggcgcca aaaattgtta atggtgtact tatataaaat gtgttaacac ctaacaaagc
      181 aaggcttacc gccaacgata tatcaggcga agctccgtga acatatctac cagttttctt
      241 tttcaaatta tctaaaacaa accttctcac gattccgtgg tataaccttg gtagtagacc
      301 aactgaaagc ccacctccgc taagcagagc acgaagtgat tcatcagcac gaattaaccg
      361 aggctcaaac gtagcagaag gataccaaaa cgcgccagcc tgttcataat agcttttccg
      421 attaaagtca atatccttcc accaataata accgccttg
//
LOCUS       DAPGRN010000386          455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPGRN010000386 DAPGRN010000000
VERSION     DAPGRN010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.394"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(33..108)
                     /locus_tag="RHW33_004972"
     tRNA            complement(33..108)
                     /locus_tag="RHW33_004972"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Lys,seq:ttt)
     gene            complement(255..330)
                     /locus_tag="RHW33_004973"
     tRNA            complement(255..330)
                     /locus_tag="RHW33_004973"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(295..297),aa:Lys,seq:ttt)
     gene            complement(334..409)
                     /locus_tag="RHW33_004974"
     tRNA            complement(334..409)
                     /locus_tag="RHW33_004974"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(374..376),aa:Val,seq:tac)
BASE COUNT          110 a          120 c          111 g          114 t
ORIGIN      
        1 ttgcgggatt atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc
       61 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta
      121 cctgaaagat ttctactcgg aaccaatctt taaaattggt gggtcgtgca ggatgactcg
      181 cttcgctcgc cctacgggcc gttgctggcg caacgttatc cttcacgctt aacatctgag
      241 tttgatgtta aaactggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
      301 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg ttcgaaccgc
      361 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
      421 ggatactaca aactacttcc accgaaccca ccgta
//
LOCUS       DAPGRN010000387          454 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPGRN010000387 DAPGRN010000000
VERSION     DAPGRN010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.395"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>454
                     /locus_tag="RHW33_004975"
     CDS             <1..>454
                     /locus_tag="RHW33_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HDV1648366.1"
                     /translation="SRALMNELFNDEIIDENDNFKDSDAYSRLKSKYPAAFPIGVKPG
                     KIKKATDGKRRTKMRVGKFSELKELWELINQKAVIEYKINSENEFLSIFKSFMLEETE
                     RFTKSGVHTRIDKIYIHNDMAMSKSIVSDDDDFAKLNTMSYREFLDNLS"
BASE COUNT          175 a           76 c           83 g          120 t
ORIGIN      
        1 atccagagca ctaatgaatg aacttttcaa tgatgaaatc attgatgaaa atgataattt
       61 caaagattca gatgcctaca gccgcttaaa atccaaatat ccagcagcat tccctatagg
      121 agtgaaacca ggtaaaatca aaaaagctac tgatggaaaa agacgcacta aaatgcgtgt
      181 tggtaagttc agtgagctga aagaactatg ggaattaatc aatcaaaaag cggtgattga
      241 gtacaaaata aacagtgaaa atgagttttt atccattttc aagtcattca tgctcgaaga
      301 aacagaacga tttaccaaat caggcgttca tactcgcatt gataaaattt acatccacaa
      361 tgatatggca atgtcgaaaa gcattgttag tgatgacgat gactttgcta agctaaacac
      421 aatgagttat cgagagtttc tcgataactt atca
//
LOCUS       DAPGRN010000388          453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPGRN010000388 DAPGRN010000000
VERSION     DAPGRN010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.396"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>453)
                     /locus_tag="RHW33_004976"
     rRNA            complement(<1..>453)
                     /locus_tag="RHW33_004976"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           83 a          145 c           98 g          127 t
ORIGIN      
        1 ccttctcccg aagttacggc accattttgc ctagttcctt cacccgagtt ctctcaagcg
       61 ccttggtatt ctctacctga ccacctgtgt cggtttgggg tacgatttga tgttacctga
      121 tgcttagagg cttttcctgg aagcagggca tttgttgctt cagcaccgta gtgcctcgtc
      181 atcacgcctc agccttgatt ttccggattt gcctggaaaa ccagcctaca cgcttaaacc
      241 gggacaaccg tcgcccggcc aacatagcct tctccgtccc cccttcgcag taacaccaag
      301 tacaggaata ttaacctgtt tcccatcgac tacgcctttc ggcctcgcct taggggtcga
      361 ctcaccctgc cccgattaac gttggacagg aacccttggt cttccggcga gcgggctttt
      421 cacccgcttt atcgttactt atgtcagcat tcg
//
LOCUS       DAPGRN010000389          447 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPGRN010000389 DAPGRN010000000
VERSION     DAPGRN010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.398"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..300
                     /locus_tag="RHW33_004977"
     CDS             <1..300
                     /locus_tag="RHW33_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III cell invasion protein SipB"
                     /protein_id="HDV1648367.1"
                     /translation="VLQASAVVLKLIAKISNGLEKIGLLICAIATSTMNCFVAGNSAD
                     MAILQQDMSNLSKTREQMLSVLQRVDKTVEQEVSQMVRVLQHRTEALKFASHSIV"
BASE COUNT          167 a           79 c           85 g          116 t
ORIGIN      
        1 gtgctacaag catcagccgt agtgttgaag ttgattgcca aaataagtaa tggcctggag
       61 aaaataggct tactcatctg tgcaatagca acatcgacga tgaattgttt tgttgctgga
      121 aactctgccg acatggcaat tttacaacag gacatgagta atctatcaaa aacgcgtgaa
      181 caaatgcttt cagtattgca aagggtggat aaaaccgtcg aacaagaggt aagtcagatg
      241 gtaagagtat tacaacaccg aactgaagcc ttaaaatttg cttcccattc tatcgtataa
      301 gagggtttta acatggccac ctatgaaatt aaaaatgcga ccagcataca taatattaac
      361 aatctcgata aaacaacctt tgatagagat tatggtaaag aaaacaacat accaaatgat
      421 aataaaattg tgcaatacta taacctt
//
LOCUS       DAPGRN010000390          435 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPGRN010000390 DAPGRN010000000
VERSION     DAPGRN010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.399"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>435)
                     /locus_tag="RHW33_004978"
     CDS             complement(<1..>435)
                     /locus_tag="RHW33_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III effector"
                     /protein_id="HDV1648368.1"
                     /translation="SYLFMQSFVQDCTIQGGYCPGSSILLSHFYNCTIAKLDAHGMDF
                     LANAFTKSSDNEARAQDQGTVFDCCNLNSIKMNCSLGNVSSDEYKFNPEEYFSDYIER
                     RSRNLKLDDNFSKCVGNIEKAVKTSFSKQNSEIDNISFNYCD"
BASE COUNT          152 a           97 c           57 g          129 t
ORIGIN      
        1 aatcacaata attaaaggag atattatcaa tctctgaatt ttgcttgcta aatgaagttt
       61 taacagcctt ctcgatattc cctacgcact tagaaaaatt atcatccaac ttaagatttc
      121 tcgatcttct ttcaatgtaa tcagaaaaat actcttcggg gttaaactta tattcatctg
      181 aactcacatt accaagacta cagttcattt taatactatt caaattacaa caatcaaaca
      241 cagttccctg atcctgcgcc ctggcttcgt tatcactaga cttagtgaat gcgtttgcca
      301 aaaaatccat cccatgagca tcaagtttcg ctatcgtaca gttataaaaa tgactcagaa
      361 gtatactcga accaggacaa tatcccccct gaattgtgca atcctgaaca aaagactgca
      421 taaagagata agaac
//
LOCUS       DAPGRN010000391          432 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPGRN010000391 DAPGRN010000000
VERSION     DAPGRN010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.400"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>432
                     /locus_tag="RHW33_004979"
     CDS             <1..>432
                     /locus_tag="RHW33_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648369.1"
                     /translation="VVSAILLSGCQSSGADYAADVYDTAQLNSKQETKTVNIISVLPA
                     KVKVDNKANKEAAQTFGAVLGAVAGGVAGYNVKGTSTLGAVAGGTGGAALGAAAGSLV
                     SDKTIVEGVSLTYKEGTKVFTSTQVGKACQFTTGLAVLIST"
BASE COUNT          109 a           75 c          116 g          132 t
ORIGIN      
        1 ttgttgtatc tgcaattctt ctttctgggt gtcaatctag cggtgctgat tatgctgccg
       61 atgtttatga taccgctcaa ttgaattcga agcaggaaac aaagacagtc aatattatct
      121 ctgtgcttcc agctaaagtg aaagttgata ataaagctaa taaagaagcg gcacagacat
      181 ttggtgcggt actgggagct gttgctgggg gcgttgccgg ttataatgtt aaaggcacat
      241 cgacactggg tgctgtagct ggcggtactg gtggtgcggc tcttggtgca gcggctggtt
      301 ctttggttag tgataaaaca attgttgaag gtgtttcact gacttataaa gaaggaacta
      361 aggtgttcac ttcaactcaa gttggtaaag catgccagtt tacaacgggg cttgctgtgc
      421 ttatttcaac ta
//
LOCUS       DAPGRN010000392          431 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPGRN010000392 DAPGRN010000000
VERSION     DAPGRN010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.401"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..181
                     /locus_tag="RHW33_004980"
     CDS             <1..181
                     /locus_tag="RHW33_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV1648370.1"
                     /translation="RHILRAFSLLRPGGVLVAVCLNGPRQQEKLLPFSDVREELPRGT
                     FAYTDVPTMIIRLRA"
     gene            207..410
                     /locus_tag="RHW33_004981"
     CDS             207..410
                     /locus_tag="RHW33_004981"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648371.1"
                     /translation="MPVSPRREKTRAGGAERGRAHAPPLSLRGRSDGGFCFFMPVICL
                     LLMMLLFLLLLLLLLLFFFFLLL"
BASE COUNT           60 a          125 c          125 g          121 t
ORIGIN      
        1 ccggcatatc ctgcgggcct tttccctgtt gcgtccgggt ggcgtgctgg tcgccgtctg
       61 tctcaacgga ccacgccagc aggagaagct gttaccgttt tctgacgtcc gcgaggagct
      121 gccgcgcggc acgtttgctt ataccgatgt cccgacgatg attattcgtc tgcgtgcctg
      181 atgacacagg gccggcggga ggataaatgc ctgtcagccc gcgccgggaa aaaacgcgcg
      241 ccggtggcgc tgaaaggggc cgcgctcacg cgccgcccct ttcattacgc ggacgttctg
      301 atgggggctt ctgtttcttc atgcctgtca tttgtctgtt actgatgatg ttgttgttcc
      361 tgctactgct actgctactg ctactgttct tctttttcct gctgctctga ttctgactac
      421 tgcagtgttg t
//
LOCUS       DAPGRN010000393          431 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPGRN010000393 DAPGRN010000000
VERSION     DAPGRN010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.402"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..423
                     /gene="espX1"
                     /locus_tag="RHW33_004982"
     CDS             <1..423
                     /gene="espX1"
                     /locus_tag="RHW33_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HDV1648372.1"
                     /translation="SCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTMIKNLRCED
                     IMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVY
                     KELKKFMHDYDKTSPEMRKYLQSINESMFLMKKISHQD"
BASE COUNT          165 a           85 c           66 g          115 t
ORIGIN      
        1 tcatgttttg aaagcgactt atcaaacacc atcttttcaa atggagaaat aaacaacctc
       61 ttcattaaaa aaagcaacat atttggaaca tcgttcacta ataccatgat aaaaaatttg
      121 cgctgtgaag atattatgcc tggccgatgg accactcaac ttgtcaataa gcacttaggt
      181 tacagataca caggagtatt caaaaccctg gcaagtattg atgacaaacc gtcccgtttc
      241 gaaattttga taccattggt acaaacttta gttcgcgaca atgtaaagct aaataatgat
      301 gtgtataaag aactgaaaaa gtttatgcac gattacgaca aaacatcccc agaaatgaga
      361 aaataccttc agagcatcaa cgagtctatg ttccttatga aaaaaatatc tcatcaagat
      421 taaaataccc c
//
LOCUS       DAPGRN010000394          428 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPGRN010000394 DAPGRN010000000
VERSION     DAPGRN010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.403"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            17..>428
                     /locus_tag="RHW33_004983"
     CDS             17..>428
                     /locus_tag="RHW33_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HDV1648373.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGKSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTIRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTE"
BASE COUNT          146 a           62 c           86 g          134 t
ORIGIN      
        1 ttagcgggtg gtattcatgg aaaataatga taaattctta tcacaagact tattggaatc
       61 ttatgccatt cgtttgttga gcggaccatt gaatggatgc gagtatgaaa tacttaatgg
      121 acgtctactt gttatcattg gtaatgatgt ttcgttagga aaaagtgatg ctttttctga
      181 gttaccagaa aatacaatag tcgttcctta tggcgagctt acaggtagtt ttgagataat
      241 aattactacc gaccctgatc tggttgtaac aatcagagaa ttaacagcac aagaacctga
      301 agatagaacg ttaacattga atcagcaaat agaagtttta ggtctcaaat ttgctgtaaa
      361 agaaaaaaat gaagtttggc agtattcatt gccaggcatt attgaaaata acattatctc
      421 tacagaac
//
LOCUS       DAPGRN010000395          420 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPGRN010000395 DAPGRN010000000
VERSION     DAPGRN010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.404"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>420
                     /locus_tag="RHW33_004984"
     CDS             <1..>420
                     /locus_tag="RHW33_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HDV1648374.1"
                     /translation="RYITVQRISEVIQRLIQERISVRNMRLVMEALALWSPREKDIIT
                     LVEHVRGALGRYICHKFSYSGEIKAIVISPEIEDRIRDGVRPTAGGTFLNLDASEAEM
                     ILDNFKLALSGINIPIKDIILLGSVDIRRFIKKLIESS"
BASE COUNT          122 a           75 c           95 g          128 t
ORIGIN      
        1 agatatataa ccgtgcagcg catttcagag gtaattcagc gtctgatcca ggagcgaatt
       61 tcagtgcgca atatgcggtt agttatggag gccttagcat tatggagccc acgagagaaa
      121 gatataatca cactcgtcga gcatgtacga ggtgcattag gtcgctacat ttgccataag
      181 ttttcatatt ctggagaaat taaagcaatt gtgatttcgc ctgaaataga ggacagaatc
      241 agagatggtg tcaggcctac cgctggcggt acttttctta atctcgatgc atccgaagct
      301 gagatgattc tggataattt taaactggca ctttctggaa tcaatattcc tattaaagac
      361 attattttac tgggatctgt ggatattcgt cgttttatca aaaaacttat tgaatctagt
//
LOCUS       DAPGRN010000396          415 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPGRN010000396 DAPGRN010000000
VERSION     DAPGRN010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.405"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   18..290
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=18..46
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          120 a          115 c           93 g           87 t
ORIGIN      
        1 aataggttat gtttagagag ttccccgcgc cagcggggat aaaccggccc tataatgaga
       61 aataaactaa ttcctattga gttccccgcg ccagcgggga taaaccgcaa aaacaatcag
      121 gagaggcgct ggcacaccag agttccccgc gccagcgggg ataaaccgat tagccgcttc
      181 actaaaccgc gcgccagtgc gagttccccg cgccagcggg gataaaccgg gtacaaaaga
      241 ggccccattc tggcagggct cgagttcccc gcgccagtgg ggataaacca ccatataacc
      301 cgttatctct ttctcaagtt tttatattag cagtactcgt aataagcaac atatccacgt
      361 aaccactcat gttcaaaata gctctccata tatgacaagt tcacaattat cgata
//
LOCUS       DAPGRN010000397          409 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPGRN010000397 DAPGRN010000000
VERSION     DAPGRN010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.406"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>409
                     /locus_tag="RHW33_004985"
     CDS             <1..>409
                     /locus_tag="RHW33_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV1648375.1"
                     /translation="SLLTSEEVILTYPAGKCLKLLLDNAYSVVSHNQFFEKVWSDSSA
                     EVATNTLYQNISMIRRSFREISSATIFNDVIRTVPRKGFKINEEISITVLDNNHNENE
                     SVESEENGTHTENINKNHIAKGIFTTKNTLLIV"
BASE COUNT          150 a           74 c           74 g          111 t
ORIGIN      
        1 catcactcct tacatcagag gaagtgatac tcacttatcc tgcgggaaaa tgcttaaagt
       61 tacttttaga taacgcttat agtgtcgtgt cacacaatca attctttgaa aaagtctgga
      121 gcgatagcag tgccgaagtc gcaaccaaca ctttatatca aaatatatcg atgataagac
      181 gttcttttcg tgaaatcagc tcagcgacga tatttaatga tgttatcaga acggtgcccc
      241 gtaaaggctt caaaatcaat gaagaaatca gcataactgt tctcgacaat aatcacaatg
      301 agaatgaaag tgttgagagt gaagagaatg gtacccacac tgaaaatata aataaaaatc
      361 acattgcaaa aggtatattt accacaaaga atacattgct tatagtttg
//
LOCUS       DAPGRN010000398          394 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPGRN010000398 DAPGRN010000000
VERSION     DAPGRN010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.denovo.407"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            86..>394
                     /locus_tag="RHW33_004986"
     CDS             86..>394
                     /locus_tag="RHW33_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV1648376.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKT
                     I"
BASE COUNT          143 a           54 c           89 g          108 t
ORIGIN      
        1 tcccgcagat gtagcgctaa taagaagaaa agatcaacta aaccatcaaa gagcatggga
       61 tatattatct gatacttatg aagatatgaa gagattaaat ttaggtggga ctgatcaatt
      121 tttccattgt atggcatttt gtcgagtgtc taaattaaat gacgctggtg ttagccgatc
      181 ggcgaaaggg ctgggttatg aaaaagagat tagagattac gggttaaatc tgttcggtat
      241 gtacggcaga aaagtaaagc tatcccattc tgaaatgatt gaagataata aaaaagactt
      301 ggctgtaaat gaccatgggt tgacatgtcc atcaacaaca gattgctcag atagatgtag
      361 tgattatatt aatccagagc ataaaaaaac gata
//
LOCUS       DAPGRN010000399        18586 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.096, whole genome shotgun sequence.
ACCESSION   DAPGRN010000399 DAPGRN010000000
VERSION     DAPGRN010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.096"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="RHW33_004987"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="RHW33_004987"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV1648377.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="RHW33_004988"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="RHW33_004988"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV1648378.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2039..3007
                     /gene="psd"
                     /locus_tag="RHW33_004989"
     CDS             2039..3007
                     /gene="psd"
                     /locus_tag="RHW33_004989"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV1648379.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3029..6352
                     /gene="mscM"
                     /locus_tag="RHW33_004990"
     CDS             3029..6352
                     /gene="mscM"
                     /locus_tag="RHW33_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV1648380.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6503..8005)
                     /gene="yjeM"
                     /locus_tag="RHW33_004991"
     CDS             complement(6503..8005)
                     /gene="yjeM"
                     /locus_tag="RHW33_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV1648381.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="RHW33_004992"
     CDS             complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="RHW33_004992"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV1648382.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9526..11334
                     /gene="frdA"
                     /locus_tag="RHW33_004993"
     CDS             9526..11334
                     /gene="frdA"
                     /locus_tag="RHW33_004993"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV1648383.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11327..12061
                     /gene="frdB"
                     /locus_tag="RHW33_004994"
     CDS             11327..12061
                     /gene="frdB"
                     /locus_tag="RHW33_004994"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV1648384.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12072..12467
                     /gene="frdC"
                     /locus_tag="RHW33_004995"
     CDS             12072..12467
                     /gene="frdC"
                     /locus_tag="RHW33_004995"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV1648385.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12478..12837
                     /gene="frdD"
                     /locus_tag="RHW33_004996"
     CDS             12478..12837
                     /gene="frdD"
                     /locus_tag="RHW33_004996"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV1648386.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12900..14033
                     /locus_tag="RHW33_004997"
     CDS             12900..14033
                     /locus_tag="RHW33_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV1648387.1"
                     /translation="MFKTTLCALLITASCSTFAAPQKINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQINLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIVNGSDNKIALAARPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14122..14655
                     /gene="blc"
                     /locus_tag="RHW33_004998"
     CDS             14122..14655
                     /gene="blc"
                     /locus_tag="RHW33_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV1648388.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYHHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="RHW33_004999"
     CDS             complement(14652..14969)
                     /gene="sugE"
                     /locus_tag="RHW33_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1648389.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RHW33_005000"
     CDS             complement(15145..15291)
                     /gene="ecnB"
                     /locus_tag="RHW33_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV1648390.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RHW33_005001"
     CDS             complement(15402..15527)
                     /gene="ecnA"
                     /locus_tag="RHW33_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV1648391.1"
                     /translation="MMKRFIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RHW33_005002"
     CDS             complement(15579..16145)
                     /gene="efp"
                     /locus_tag="RHW33_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV1648392.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16187..17215
                     /gene="epmB"
                     /locus_tag="RHW33_005003"
     CDS             16187..17215
                     /gene="epmB"
                     /locus_tag="RHW33_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV1648393.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RNAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFAIAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17610..18479
                     /locus_tag="RHW33_005004"
     CDS             17610..18479
                     /locus_tag="RHW33_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV1648394.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQQNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4681 a         4847 c         4752 g         4306 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 ggcgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 agcaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctgaccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcagactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacacgcccg gatttctatg acggcgtgaa acccatcgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc actcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgaacagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggcaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgcac gcctgtatca cttccctcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaaggaaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 tcaggatccg gcatgtaggc cggatcagga acgacaaaac aatggcctgg aggctacctt
     2041 gttaaattca tttaaacttt cgctacagta cattctgccg aaactatggc ttactcgcct
     2101 ggcgggttgg ggcgcaagca agcgggcagg atggctgaca aaactggtta tcgatctgtt
     2161 cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag ccggacaccg ccagctaccg
     2221 cacctttaac gaattctttg tccgtccgct gcgtgacgaa gtacgcccaa tcgataccga
     2281 tccgaatgtc ctggtcatgc ctgccgatgg cgttatcagc cagttgggta aaatcgaaga
     2341 agataaaatc ctgcaagcca aaggccacaa ctacagcctc gaagccctgc tggcaggcaa
     2401 ctatctgatg gcggacctgt tccgcaacgg tacgtttgtg accacttacc tctccccgcg
     2461 tgactaccac cgcgtacaca tgccgtgcaa cggtattctg cgtgagatga tctacgtgcc
     2521 gggcgatctc ttctccgtta accatctcac ggctcagaac gtgccgaatc tgtttgcccg
     2581 taacgaacgc gtgatctgcc tgttcgatac cgaatttggc ccaatggcgc agattctggt
     2641 cggggcgacg attgtaggca gcattgagac tgtctgggcg ggcaccatta cgccgccgcg
     2701 cgaaggcatc atcaagcgct ggacctggcc tgccggggaa aacgacggtt ctgtggcact
     2761 gctgaaaggc caggaaatgg gtcgctttaa actcggttcc accgttatca acctgtttgc
     2821 accaggtaaa gtgaatctgg ttgagcaact ggaaagcctg tctgtcacga aaattggcca
     2881 gccgctggca gtatctaccg aaacctttgt tacgccagac gctgaacccg ccccgcttcc
     2941 tgctgaagag atcgaagcag aacacgacgc cagcccattg gttgacgata aaaaagacca
     3001 ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt atcacttttc tgatggcctg
     3061 gtgcctcagt tggggggcgt acgccgcgac ggcccccgat agcaaacaaa tcacccagga
     3121 actggagcag gcaaaagcgg cgaaacccgc gcagccagaa gtcatagagg cactccagtc
     3181 tgccttaaat gcgcttgagg aacggaaagg ttcccttgag cgcatcaagc aatatcaaga
     3241 agttattgat aattatccga aactctccgc tactctgcgc gcacaattaa acaacatgcg
     3301 tgacgagccg cgcagcgtgt cgccggggat gtctaccgac gcgctgaatc aggaaattct
     3361 ccaggtcagc agtcagttgc tggataaaag ccgtcaggcc cagcaagagc aggagcgcgc
     3421 ccgcgagatt gccgattcgc tgaatcaact gccacaacag caaaccgacg cccgccgtca
     3481 gttaaacgag atcgagcgcc gcctggggac gcttaccggc aatactccgc tcaatcaggc
     3541 acaaaatttc gcgttgcagt ctgactctgc acgtcttaag gcgctcgttg atgaactgga
     3601 gctggcgcag ctgtctgcca ataaccgcca ggaattagcg cgcttacgct cagagcttgc
     3661 ggaaaaagag agccagcaac tggatgcgta tttgcaggcc ttacgtaatc aattaaacag
     3721 ccaacgtcag cttgaggcgg agcgggcgct ggaaagtacc gaattgctgg cagaaaacag
     3781 tgccgatttg ccgaaagaca tcgtcgcgca attcaaaatt aaccgcgaac tgtcggcggc
     3841 tttgaatcaa caggcgcagc ggatggatct cgttgcctcg caacagcgtc aggctgccag
     3901 ccagacgtta caggtccggc aggcgttgaa tacgctgcgt gaacagtcgc aatggctggg
     3961 atcgtccaat ctgctcggtg aagcgttgcg ggcgcaggtg gcacggctgc cggaaatgcc
     4021 gaaaccacaa cagcttgata ccgaaatggc gcagttgcgt gtgcaacggt tacgttatga
     4081 ggatctgctt aataaacagc cgctgctacg gcaaattcat caggccgacg gtcagccgct
     4141 gactgccgag caaaaccgta ttctcgaagc acagctacgc actcagcgtg agttgctgaa
     4201 ctcattgttg cagggtggcg acacactact gctggaactg accaagctga aagtctccaa
     4261 cgggcaactg gaggatgcgc tgaaagaggt gaacgaagcg acgcaccgct atctgttctg
     4321 gacctctgac gtgcgcccga tgaccatcgc ctggccgctg gaaatcgccc aggatctgcg
     4381 tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa gccagtgtga tgatgctgac
     4441 cagcaaagag acgattttgc cgctgtttgg cgcgttgatt ctggtcggtt gcagtattta
     4501 ctcgcgccgc tattttaccc gttttctcga acgttcggcg gcgaaagtcg gcaaagtgac
     4561 tcaggatcac ttctggctga cgttgcgcac tcttttctgg tcgattctcg tcgcgtcacc
     4621 gctgccggtg ctgtggatga cgctgggtta cggcttgcgc gaggcgtggc cttatccgct
     4681 ggcggtcgcg attggcgatg gcgtaacagc caccgtgccg ctgctgtggg tagtgatgat
     4741 ttgcgctact tttgcccgcc cgaacggctt gtttatcgct cattttggct ggccgcgcga
     4801 acgtgtttct cgtgggatgc gctactacct gatgagcatc gggcttattg tgccactgat
     4861 tatggcgctg atgatgttcg ataacctcga cgaccgcgaa ttttccggtt cgctgggacg
     4921 gctttgcttt atcctcattt gcggtgcgct ggcggtggtc accctcagcc tgaaaaaggc
     4981 cgggataccg ctgtatctca acaaagaggg cagcggcgac aacattacca accatatgct
     5041 gtggaacatg atgattggcg cgccactggt tgccattctg gcgtcggcgg tgggttatct
     5101 ggcaacggcg caggcactgt tagcgaggct tgaaacctcg gttgccatct ggttcctgct
     5161 actggtggtt tatcacgtta tccgccgctg gatgctgatc cagcgccgca ggctggcgtt
     5221 tgaccgggcg aagcatcgcc gggcagagat gttagcgcaa cgcgcgcgtg gcgaagagga
     5281 agcgcatcat cacagtagcc cggaaggggc aattgaagtc gatgaaagcg aagtcgatct
     5341 cgatgccatt agtgcgcaat ccttgcggct ggtgcgctca attttgatgt tgatcgccct
     5401 gctttcggtc attgtgctgt ggtcagaaat ccattccgct ttcggcttcc tcgaaaatat
     5461 ttcgctgtgg gatgtcacct ccacggtaca gggcgtagaa agtctggagc caattaccct
     5521 cggtgcggtg ctgattgcca ttctggtgtt tatcatcacc acgcaactgg tgcgcaacct
     5581 gcccgcgctg ctggaactgg cgattttgca gcacctggat ttaacgccgg gtacgggtta
     5641 cgccatcacc accatcacca aatatctgct gatgctgatt ggcgggctgg tcggcttctc
     5701 aatgattggt attgagtggt cgaaattgca gtggctggtt gccgcgctcg gtgttggtct
     5761 cggttttggt ttgcaggaaa ttttcgccaa ctttatctct ggcctgatca tcctgttcga
     5821 aaaaccgatt cgcattggcg atacggtaac gatccgcgat cttactggta gcgtgacaaa
     5881 aattaacacc cgcgccacca ccatcagcga ctgggaccgt aaagagataa tcgtgccgaa
     5941 caaggcgttt attaccgagc agtttatcaa ctggtcgctc tctgactcgg tcacgcgcgt
     6001 ggtattgacg attccggccc ctgccgatgc caacagtgaa gaagtgacgg aaatcctgct
     6061 caccgcagcg cgtcgctgct cgctggtgat cgacaacccg gcaccggaag tcttcctggt
     6121 ggatctgcaa caggggattc agattttcga gctgcgtatt tacgccgctg agatgggtca
     6181 ccgtatgccg ctacgccatg agatccacca gctgattctg gctggcttcc atgcccacgg
     6241 tatcgatatg ccattcccgc ccttccagat gcgtctggaa agcctcaacg gtaaacaaac
     6301 ggggagaacg ctgacgtctg cgggcaaagg tcgtcaggcg ggaagtttgt aatgcatgaa
     6361 gctgccaaat gatgtcaacg tggctcattt agcagcttaa catcatcttc agttatcacc
     6421 ggccctggag agtatcatcc atacgcatta cttcctgaca actcatcgcc ggagagacga
     6481 tgagttgcag gatttcatat gatcagtcga gagctaactc aggtttattc gccatgcgac
     6541 tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg ccgccaatca
     6601 tccacaatgt gctgtcccag tctccggctt caaccacagg ctgaataatg gtgaagacgt
     6661 tcgcaaatgt caccaccaga acgacaacca ccgtcgcaat cattgccgac atacgcgttt
     6721 tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt aaagaatggg aaagccagcg
     6781 cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc ttgttaaaga
     6841 acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat gaagacagta accaacccgc
     6901 actgcatcca catggcgata gacggcatcc ccatcgcatt caggcgcgtc atcggttccg
     6961 gccacaacgc tttcggcgtc ccctggatga tggctttcag cggtgaatag caaagcgtaa
     7021 agaacgcacc ggtataagcg aggaacatcg aaagtccggt aatacgcgca aaccatacgc
     7081 ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt acccagcgtc accccgaggc
     7141 tcttcatcag cacataggta atattgccaa ggttaacgga accattactt aatacctgct
     7201 gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt gaaataacaa
     7261 tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt ttatcgacca
     7321 gaccaccaac cgcttcaatt ccgccatagg caaaaatagc aaataccaca aacgatagca
     7381 ttgccagacc ggactgataa cccgggttcg gtgatgcaag gaaattaata tcctgcgcga
     7441 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
     7501 acattactgc aataccgccc accgcagtaa tgcgggcaat tttatttatc cccttagaag
     7561 caacgacggt gaccagaatc atccatgcca ctgccagcag accaaccacc tgcgtaggct
     7621 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
     7681 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
     7741 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
     7801 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
     7861 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
     7921 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
     7981 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataaaacg
     8041 cgcccactga actgcactgc ccaacattga gcgggcagcg taaaaaccaa tacagaattg
     8101 atacctggat gatatcaaat atccagtctt aaattgtggc ctttagcgga aaaatgaaaa
     8161 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
     8221 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cccccagcgc
     8281 caacatcacc agacgatcaa cgcctaatgc cacgccggaa cagtcaggca taccgacttt
     8341 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
     8401 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt catggaaacc
     8461 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     8521 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     8581 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
     8641 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgccactt cccgcagttg
     8701 cgttttgtcg gcagagagtg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     8761 aaggctttct gctgccgggc agtccagtac ctgttgtaag aggtcgtcca cctcgttcat
     8821 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     8881 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     8941 agcaaccagc aggcgtttca tatggtattc agggctggtc attaaccaga gattcatccc
     9001 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     9061 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     9121 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     9181 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     9241 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acaacggtgg gcagtgacta
     9301 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     9361 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     9421 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     9481 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     9541 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     9601 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     9661 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     9721 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     9781 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     9841 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgtacc
     9901 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     9961 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
    10021 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
    10081 gctaacgcag tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
    10141 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
    10201 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
    10261 gaaggttgcc gtggcgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
    10321 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
    10381 ggtccacgcg ataaagtctc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
    10441 tccacgccgc gtggcgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
    10501 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
    10561 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
    10621 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
    10681 ctgcacggtg caaaccgtct gggttctaac tccctggcgg aactggtggt cttcggccgt
    10741 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
    10801 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
    10861 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg ctatggaaga aggctgcggt
    10921 atttaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
    10981 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtat tcaacaccga cctgctctac
    11041 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
    11101 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
    11161 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
    11221 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
    11281 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
    11341 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
    11401 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
    11461 agacaacctg gcaccggacc tgagctaccg ctggtcctgc cgtatggcga tttgtggttc
    11521 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
    11581 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
    11641 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
    11701 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcca agtatcacca
    11761 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
    11821 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
    11881 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
    11941 tactttcgtg ggctactgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
    12001 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
    12061 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
    12121 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
    12181 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
    12241 gggcgggatt cgtcgacttt ttacaaaacc cggttattgt gatcattaac ctgatcactc
    12301 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
    12361 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
    12421 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
    12481 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
    12541 ggtatgtgga gcgccatcat tgcgccggtg atgatccttc tggtgggtat tctgctgcca
    12601 ctggggctgt ttccgggtga tgcactgagc tacgagcgcg ttctggcgtt cgcgcagagc
    12661 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
    12721 cgtatgcacc acgcgatgca cgatctgaaa atccacgtac ctgcgggcaa atgggttttc
    12781 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
    12841 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacccta
    12901 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
    12961 cccaaaaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
    13021 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
    13081 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
    13141 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
    13201 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aacgggatca
    13261 cgctattaca tctcgcaacc tacaccgctg gcggcctgcc attgcaggtg ccggatgagg
    13321 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
    13381 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
    13441 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
    13501 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
    13561 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
    13621 cgaccattga agatatggcc cgctgggtgc aaatcaattt aaaacccctt gatatcaatg
    13681 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
    13741 tgtatcaggg tctgggctgg gaaatgctgg actggccggt aaatcctgac atcatcgtta
    13801 acggcagtga caataaaatt gcactggcag cacgccccgt aaaagcgatt acgcccccaa
    13861 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
    13921 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
    13981 ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctgcag taaaatttca
    14041 tcgggtccga attttcggac cttttctccg cttttccttg ctgtcatcta cacttagaaa
    14101 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    14161 tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtgaccgt agtgaataat
    14221 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgcttt
    14281 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
    14341 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
    14401 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    14461 ggcggttata acgttattgc actcgatcgg gaataccacc atgcgctggt ttgcggcccg
    14521 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacaa
    14581 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    14641 cagcccggta gttaatgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    14701 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
    14761 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
    14821 atcgcccagg caagtagcgc catactgacg atcatcgccg tcacagtaat aacacttggc
    14881 gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
    14941 ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg gccgtccccg
    15001 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
    15061 ggtggcaggg gaatttcaac tattaccttt gtgactatcg cagaaaaaac aaacggcacg
    15121 acacagctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcac cagaaatcgc
    15181 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
    15241 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
    15301 cttttagata atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
    15361 aaatgacgga aatctgatga tttttcggag gagaagaaaa attagctggc agcgcgggag
    15421 atggagttgc cgagatgttt gatgtcttcg ccgaaaccgc gagcggtgtt acagcccgtg
    15481 agcagcgtgc tggcaagcaa aacaagaacg ataaagcgtt tcatcatctc tgacctgaat
    15541 aagtgatggt gcagcctgta ggccgcacca caaccgcatt acttcacgcg agagacgtat
    15601 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
    15661 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
    15721 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
    15781 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcgttg
    15841 tcgccaattg cttttgcgtc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
    15901 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
    15961 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
    16021 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
    16081 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
    16141 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
    16201 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
    16261 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
    16321 caacgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
    16381 aggcaatccg gacgatccgc ttttgcgtca ggtacttacc tcgcaagatg agtttgccat
    16441 cgcgcccgga ttctccaccg acccgctgga agaacagcac agcgtagtgc ctggtttgtt
    16501 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
    16561 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
    16621 aactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
    16681 cgatccgctg atggcgaaag atcacgagct ggactggttg ctcacacaac tggaagccat
    16741 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
    16801 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
    16861 catcaatcat gccaatgagg tagatgaaac attccgtcag gcgatggcta agttgcgccg
    16921 ggtaggcgtt actttgctga accagagcgt tctgttacgt gatgtgaacg ataacgcaca
    16981 aacgctggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
    17041 tgtgctcgat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
    17101 gattatgcgt gagttgctga cactggtgtc gggatatctg gtgccgaaac tggcgcgaga
    17161 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
    17221 taaacaggga agtcaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
    17281 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
    17341 tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaacaggga agtttaatct
    17401 ttgcccgttg cataaccttt aattattcat atgcattgct gttgttactc tttatataac
    17461 taacaccaat aattaacctt tcttttctgt ttatatagag tgagtacggc tatttatgtt
    17521 attttaacaa ctattactac caagcccatt tatctggctt ataaattaaa attaattatt
    17581 taattgagaa atggttaggg agaacctaca tggccataag catcaagggt gttaatactg
    17641 gtgttattcg caagagtaat aatttcattg cactggcgct gaaaattaaa gaacctcgca
    17701 ataaagaatc gttgttcttt atgtctgtca tggaactgcg cgacctgctg attgcgctgg
    17761 aaagtcgtct gcatcaaaag cataaactgg atgctgctgc acgccttcag tatgagcaag
    17821 ctcgcgataa agtcattaag aaaatggcag aaaacatccc agaaattctg gttgatgagc
    17881 ttaaaaatgc cgatattaat cgtcgggtta acacgctgga acttaccgat aatcaaggcg
    17941 aaaatcttac cttcgtatta acgctacatg acggtagcaa atgtgagtta gtcgtcaatg
    18001 agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc aattaataat gcggaaatgc
    18061 gtgaactggc attacgtatt acctcactgt tagatttcct gccgttatat gacgttgact
    18121 gccagcaaaa tggcaatctg gaatatgaca cctattccca accggaatgg aaacataact
    18181 tgttcgacca ttatctggcg gtgctttacc gctttaaaga tgaaagtggc aaagaacaat
    18241 ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg taaagaaata gaggcgatca
    18301 ctcgccgaat gcttgatttc agcccacgac tgaaaaaact cgtgggtgtg ccatgtcagg
    18361 tctatgttcg caccgtagcg gcgaataacg cccaaccgct aacccaggat caatgcttac
    18421 gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac tgcaccacag gcgaaataat
    18481 ctcatctttg ctaatgcgag gctggagcca tccagcctga aatgactgat aacgtgatcg
    18541 ttggtcatgc cggatgcggc gtaaacgcct tatccggcct acaaat
//
LOCUS       DAPGRN010000400        15704 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.110, whole genome shotgun sequence.
ACCESSION   DAPGRN010000400 DAPGRN010000000
VERSION     DAPGRN010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.110"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..473
                     /locus_tag="RHW33_005005"
     CDS             <1..473
                     /locus_tag="RHW33_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1648395.1"
                     /translation="KGGSWFFTVNLRNRRSQLLTTQYQTLRNAIIKVKRARPFEINAW
                     VVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHAIRNTKDYRH
                     HVDYIYINPVKHGWVKQVCDWPFSTFHRDVARGLYPIDWAGDITDLSAGERIIL"
     gene            complement(682..2394)
                     /locus_tag="RHW33_005006"
                     /pseudo
     CDS             complement(682..2394)
                     /locus_tag="RHW33_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2366..3151
                     /gene="lafU"
                     /locus_tag="RHW33_005007"
     CDS             2366..3151
                     /gene="lafU"
                     /locus_tag="RHW33_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV1648396.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            3222..4277
                     /gene="dinB"
                     /locus_tag="RHW33_005008"
     CDS             3222..4277
                     /gene="dinB"
                     /locus_tag="RHW33_005008"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV1648397.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            4274..4726
                     /locus_tag="RHW33_005009"
     CDS             4274..4726
                     /locus_tag="RHW33_005009"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV1648398.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASEHYSPQQIAVWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFTNFYMRYKPQH"
     gene            4972..5175
                     /locus_tag="RHW33_005010"
                     /pseudo
     CDS             4972..5175
                     /locus_tag="RHW33_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            5174..5695
                     /locus_tag="RHW33_005011"
                     /pseudo
     CDS             5174..5695
                     /locus_tag="RHW33_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(5752..7209)
                     /gene="pepD"
                     /locus_tag="RHW33_005012"
     CDS             complement(5752..7209)
                     /gene="pepD"
                     /locus_tag="RHW33_005012"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV1648399.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPTK"
     gene            7470..7928
                     /gene="gpt"
                     /locus_tag="RHW33_005013"
     CDS             7470..7928
                     /gene="gpt"
                     /locus_tag="RHW33_005013"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV1648400.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8020..9264
                     /gene="frsA"
                     /locus_tag="RHW33_005014"
     CDS             8020..9264
                     /gene="frsA"
                     /locus_tag="RHW33_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV1648401.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            9322..9723
                     /gene="crl"
                     /locus_tag="RHW33_005015"
     CDS             9322..9723
                     /gene="crl"
                     /locus_tag="RHW33_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV1648402.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(9762..10817)
                     /gene="phoE"
                     /locus_tag="RHW33_005016"
     CDS             complement(9762..10817)
                     /gene="phoE"
                     /locus_tag="RHW33_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV1648403.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            11104..12207
                     /gene="proB"
                     /locus_tag="RHW33_005017"
     CDS             11104..12207
                     /gene="proB"
                     /locus_tag="RHW33_005017"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV1648404.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            12219..13472
                     /gene="proA"
                     /locus_tag="RHW33_005018"
     CDS             12219..13472
                     /gene="proA"
                     /locus_tag="RHW33_005018"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV1648405.1"
                     /translation="MLEQMGIAAKQASYKLAQLSCREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            13587..13662
                     /locus_tag="RHW33_005019"
     tRNA            13587..13662
                     /locus_tag="RHW33_005019"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13620..13622,aa:Thr,seq:cgt)
     gene            complement(13789..14688)
                     /locus_tag="RHW33_005020"
     CDS             complement(13789..14688)
                     /locus_tag="RHW33_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV1648406.1"
                     /translation="MKIDLNLLPIFIAVAEESNFSKAAARLGVTRSAVSQGIRRLEDA
                     FGTMLVMRTTRSVNLTEAGERLHKSLSLPLSGIEAAFEEMVSDHVPRGHLRIAVTSIA
                     EEFLSGPLIASFAAANPAVTLDIVVTDEEFDIVAAGYDAGVRLGEVIEKDMIAVPLTG
                     KQREMVVASPSYLAANSTPVHPRELVNHKCIGWRQSPDVAPYRWPFEENGRAFDLAIE
                     PQITTNDLRLMLRLALAGGGITIATQETFRPYIESGKLVSLLDDFLPQFPGFYLYFPQ
                     RRNIAPKLRALIDHVKEWRQQLA"
     gene            14787..15509
                     /locus_tag="RHW33_005021"
     CDS             14787..15509
                     /locus_tag="RHW33_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV1648407.1"
                     /translation="MDKVILITGASSGIGEGIARELGTTGAKTLLGARRVERIEAIAT
                     EICRAGGIAKARELDVTDRQSMADFVQVALDSWGRVDVLINNAGVMPLSPLAAGKQNE
                     WELTIDVNIKGVLWGIGAVLPVMEAQGSGQIINLGSIGALSVVPTGAVYCASKFAVRA
                     ISDGLRQESSKIRVTCVNPGVVESELASTITHVETMKAMDAYRSVALQPADIARAVRH
                     IIESPESVDTTEITIRPTASAN"
BASE COUNT         3844 a         3926 c         4104 g         3830 t
ORIGIN      
        1 tcaagggggg aagctggttt ttcacggtga atttacggaa tcgtcgaagc caacttttga
       61 ctacccagta ccagacgctc cgtaacgcca ttattaaagt taagcgagcc agaccttttg
      121 aaattaatgc ctgggtagtt ttgccggagc atatgcactg tatctggaca ttacctgaaa
      181 gcgatgatga tttttcctcg cgctggagag agattaaaaa gcagttcacc catgcttgcg
      241 gattgaaaaa tatctggcaa ccgcgttttt gggagcatgc catccgtaat acaaaagatt
      301 atcggcatca tgttgattat atttatataa atccagtgaa acatggctgg gtaaagcagg
      361 tgtgtgattg gccattctca acgttccatc gcgatgttgc gcgagggtta tatcccatcg
      421 attgggcggg agacataacg gatttgagtg ctggggagcg tatcatttta taattgtgcg
      481 cagatgcctg atgcgacgct agcgcgtctt atcatgccta caaacttgtg ccgcatccgg
      541 caattgtgcg cggatgcctg atgcgacgct ggcgcgtctt atcatgccta caaacctgtg
      601 ccggatcggt aggccggata aggcgttcac gccgcatccg gcaattgtgc accaatgccg
      661 gatgcgcccc ttaaaggcca atcaactcaa cgcccccata atcttcaact ccagctcatc
      721 cggcacttcg ttatacgaca gcacatgcag ccccggcgca aacaaccttg cgtagcgcgc
      781 cagcaaaggg cgcagctgcg gcggcaccag cagcaccggg tctttccccg ccgctttcat
      841 ctgctctttc acctgcggca tcgtgctctg gaactggttg agcatgttcg ggtcgaccgg
      901 cacgctgtcg agcatcactt tgccgccctg ttgcgcctga ttcaccacgt tggtcagcag
      961 attttccagc tcattattca gcgtatacac cgtcagctcc tgcttgcgaa cgaacggatg
     1021 ggtaatgctg cgccgcagcg ccaggcgcac atcagctgcc agcagaatat gatctttggt
     1081 tactgtgcta cttgcgacca gcacggtggc gatggtgacg atatcgcgca gggaaacgcc
     1141 ttcggtcagc agcgcccggt acactttcag caattggctg taattgagcg ccgcgcttaa
     1201 atcctccgcc aggcgcggtg ccatcgacga caaacggtta tgcaactgcg tgatgtcatc
     1261 atagttaaac aaatcaggaa tatagctgcg gacaatcttg ttcacatggg tggcaatcac
     1321 gctggcgctg tcgatcacct gataccccat attcagcgct ttcgctttct gcgcgggctg
     1381 gatccaggtc accggcatcc cgtacgccgg gtcgttcccc agcacgccgt ctatctcgcc
     1441 gtaggtttcg ctggagggca gggccatcag tttatccgcc ggaatatctg cttcatcagc
     1501 cttaataccg ttgatgaaaa tggcgtactg gctcggctta aggcggaagt tttcccgaat
     1561 gcggatctcc ggcagcagca cgccgttacc gtcggatatc acctgccgta cgccgcgaat
     1621 acgctgggtg agcgggttgc cctgcgcttt gtccaccagc gccaccagct tgtagccgag
     1681 gcttaaactg ataggctcga taagcggaat ggtttcccaa ctgacctgtt gctcgctggt
     1741 ttcggtgatg gtgcgggtca gtgtttcgag gcttttctct tccgcctctg ctgcctgcgg
     1801 gcgtttgctc attcgccagc cggtaaagcc aagcagggcg ctgaacagca aaaacggcag
     1861 gtgcggcatc cccggtacca ccgccagcac gaacatgatc ccggtagcgg tgtagagcac
     1921 cgacgggctt gccagcagct ggtggcgcac gtcatgggcg atatcgccgt tgtcgctgac
     1981 gcgggtgacg ataatcgccg ccgcggtgga gagcagcagg gaagggatct gcgccaccag
     2041 cccgtcgccg atggtcatca gcacatattg ctggaaggcg gcgtcggcgc tcaggttgta
     2101 tttaaagatc ccgatacaga cgccgccgat caggttgatc gccagaatca tcatcccggc
     2161 gatggcatcc ccgcgcacaa actttgatgc cccgtccatc gcgccgtaga aatcggcctc
     2221 gctggcgaca tctttacgcc gggtttgcgc ctgcgcctgg ttgatcaacc cagcgttaag
     2281 atcggcgtca atcgccatct gcttgccggg catcgcgtcc agggtaaagc gggcagaaac
     2341 ctcggaaata cgctcggccc ctttggtgac gacgataaag ttgattgtca acagcgtcag
     2401 taaatctgag cgtgaaagca ttatcgccgc gctgcacggg cagtcgattt tcaacggtgg
     2461 tgggctgtcg ccgttaaata aaatcagccc gtctcatccg ccaaaacctg ccaccgtggc
     2521 ggtgccggaa gagacggaga aaaaagcgcg tgatgtgaat gagaaaacgg cgctgctgaa
     2581 gaagaaatcc gccaccgagc ttggcgagct ggcaaccagt atcaacacca ttgcccgtga
     2641 tgcgcatatg gaagcgaatc tggagatgga gattgtcccg cagggattac gcgtgctgat
     2701 taaagacgac cagaatcgca atatgttcga acgcggcagc gcgcagatta tgccgttctt
     2761 taaaactttg ctggtggagc tcgcgccagt gttcgactcg ctcgataaca aaattattat
     2821 taccgggcat accgatgcga tggcctacaa aaacaatatc tacaacaact ggaacctttc
     2881 gggcgatcgc gcgctttcag ctcgtcgggt gctggaagag gccggaatgc cggaagataa
     2941 agtgatgcag gtaagcgcaa tggcggacca gatgctgctg gatgccaaaa atccgcaaag
     3001 cgcgggcaat cggcgtattg agattatggt gctgaccaaa agtgcgtccg atacgctgta
     3061 tcaatacttt ggtcagcatg gggataaagt ggtgcagccg ctggtgcaaa agctggataa
     3121 gcagcaggtg ctttcgcagc gaatgcgcta aatgctgaat ctttacgcat ttctcaaacc
     3181 ctgaaatcac tgtatacttt accagtgttg agaggtgagc aatgcgtaaa atcattcatg
     3241 tggatatgga ctgctttttc gcagcggtgg agatgcgcga caatcccgcc ctgcgcgata
     3301 tccctattgc tattggcggt agccgcgaac gtcggggggt gatcagcacc gccaattatc
     3361 ccgcgcgtaa atttggcgta cgtagcgcta tgccgacagg gatggcgctc aaattatgcc
     3421 cacatctcac cttgcttccg gggcgctttg acgcctacaa agaagcctca aatcatatcc
     3481 gtgaaatctt ctcgcgctac acctcgcgta ttgaaccgtt gtcactggat gaagcctatc
     3541 tcgatgtcac tgatagcgtc cattgccacg gttctgcgac cctcattgcc caggaaatcc
     3601 gccagacgat tttcaacgag ctgcaactga cggcgtctgc gggcgttgcg ccggtaaagt
     3661 ttctcgccaa aatcgcctcc gacatgaata aacccaacgg ccagtttgtg attactccgg
     3721 cagaagttcc ggcatttctg caaaccttac cgctggcaaa aatccccggc gtcggcaaag
     3781 tctcggcggc aaaactggaa gcgatggggc tgcggacctg cggtgatgta caaaagtgtg
     3841 atctggtgat tctgcttaaa cgctttggca aatttggccg cattttgtgg gagcgtagtc
     3901 aggggattga cgagcgcgac gttaacagcg aacggttacg aaaatccgtc ggcgtggaac
     3961 gcacgatggc ggaagatatt catcactggt ctgaatgtga agcgattatc gagcggctgt
     4021 atccggaact tgaacgccgt ctggcaaagg taaaacctga tttactgatt gctcgccagg
     4081 gggtgaaatt aaagttcgac gattttcagc aaaccaccca ggagcacgtc tggccgcggc
     4141 tgaataaagc tgacttaatc gccaccgcgc gtaaaacctg ggatgaacgc cgcggcgggc
     4201 gcggtgtgcg actggtgggg ctgcatgtga cgttgcttga ccctcaaatg gaaagacaac
     4261 tggtgctggg attatgaata acatacaaat aagaaactat cagcctggcg attttcagca
     4321 actatgcgct attttcatta gagcggttac gatgacagcc agtgagcatt attcaccaca
     4381 acaaattgcc gtctgggcgc agattgacga atctcgctgg aaggagaaac tcgcgaaatc
     4441 acaagtgcgg gttgcagtca ttaatgcaca accggttggt tttatttccc gcattgaaca
     4501 ttatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg ccagtgcttt
     4561 gttaaaacct ttgattaagt ctgaatccga acttacagtg gacgcaagca taaccgcaaa
     4621 accttttttt gaacgttatg gttttcagac agttaagcag cagcgcgttg aatgccgggg
     4681 agcgtggttt actaattttt atatgcgata taaaccgcaa cattaaatcc agcttgcaat
     4741 gaaaataacg cccgcctggt atgtgcgcgt tttctgattc cacaaactgc aaggaggtaa
     4801 atcatgacaa atcctttatc aatgactctt tgcagatctt tccaggatta attctttttt
     4861 tcttgccctg gattcgtctg ccatttcctg atttttatat ttatatactc taaataattc
     4921 gagtcgcagt tcatgcaact tgaagtatga cgagtataaa tggaatggta catgggcaaa
     4981 tatattcgtc ccttatccga tgcggtattt accatcgcat ctgatgacct gtggatcgag
     5041 agtttagcga tccaacaatt acacaccacg gcaaatttac ccaacatgca gcgcgtagtg
     5101 gggatgccag atttacaccc cggacgcggc tacccgattg gcgcagcgtt cttctctgtt
     5161 ggtcgttttt acccgacaag acgtcgcggt aacggtgctg gaaacagaaa cgggccgcta
     5221 ctctgacacg ctgcgttcgg cgctgatttc tctggatggc gacaacgcat gggcgttaag
     5281 cgaaagttgg tgcggcacta ttcagtggat ttgtccgagt ccgtatcggc ctcatcatgg
     5341 acgcaaaaac tggtttctgg gcattgggcg ttttactgct gatgagcagg agcaatccga
     5401 cgcggtccgt tatgagacgc tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac
     5461 cgactcggcg gtacgcgcca cgcatctggc taccggaatt agcgtgaagg ttcagtcaga
     5521 gcgcagccag catgccaaca aacggctggc gcgattgctg attgcctgga ggctggagca
     5581 gcagcaacag gaaaatagcg cggcgctgaa atcacagcgg cgaatgatcc atcaccagat
     5641 tgaacgtggc aacccgcgac ggacatttac agggatggct tttatcgaag gataatgaag
     5701 gatgaaactg ccggaaggtg attgaacgcc atccggcagc aaatcaaata attacttcgt
     5761 cggaatttct ttcagcagtt cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg
     5821 aacttgctca tccggagagt gtggaccggt gatagttggc ccgatagaaa ccatgtccat
     5881 ttccggatac ggttttttga acagaccaca ttccaggccc gcgtggataa tctggatgtt
     5941 cggcgtcttg ttgaacaggc gctgataggt ttcacgtacc agatgcatca ccggagaatt
     6001 agcgtccggc tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt
     6061 acccagcgaa tccagcatgc tcaccacgta gtctttaccg ctgtcgatca gagaacggat
     6121 cagacagtga atttctacgt tattgtcagt catggtcacc acaccgacgt tcagggaggt
     6181 ttcgaccaca ccttttgcca cgtcggagtt gcggatcaca ccgttcgggg tggcgttcag
     6241 cagacgaata aaggtatcgc gagatttcgc aatcagggca gctttatcgt tcgctacaga
     6301 gtccagcaac aaggccagat ttttctcttt ctctgccagc tcgtttttca ggatctcctg
     6361 ataggtattc accagagatt tcagggcgtc gactttatca gctgcgacag caatggtcgc
     6421 aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcaa taagacgcag
     6481 atccagttct tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg cattacccag
     6541 gccaacgtgg atttcaccgc cggagtgacc gcctttcaga ccttttaagg ttaacttgaa
     6601 ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc aggttggagg tgaagtcgat
     6661 accccccgca caacccatgt agatttcacc ttcttcttcg gagtcggtgt taatcagaat
     6721 atcagcctgc aaccagttgc tctgtaagcc aaaagcaccg tccataccgg cttcttcggt
     6781 catggtcagc agcacttcca gcggaccgtg aaccacgttt tcgtcagcca gaaccgccag
     6841 tgcagaggcc ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca
     6901 ttcgccatca atatatggct ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt
     6961 tttctgcggc accatatcga ggtgggcctg caagacgacc ggtttacgat tttccatacc
     7021 tgctgtagca ggtttacgaa tcaggatatt acctacctga tcgcgttcga catggaaacc
     7081 tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat aggacgggtg
     7141 aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata attgagacag
     7201 ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag gtcacagggt
     7261 tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtttgaga ataccacttt
     7321 atcccgcgtc agggagaggc agtacgtaaa aagacgcgga ctcatgtgaa atactggttt
     7381 ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag
     7441 ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca
     7501 tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag
     7561 gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg cgtgaactgg
     7621 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     7681 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     7741 tagataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     7801 ccatctttgc aaaaccggct ggtcgtccgc tggttgatga ttatgttgtt gatatcccgc
     7861 aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     7921 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     7981 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     8041 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     8101 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     8161 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     8221 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     8281 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccaccca ggcgatggtg
     8341 tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca gtggtcgtca ctggctgcat
     8401 gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga cctggccgag
     8461 caagcgcagg ctttgtcaaa ccgcgcctat gaagaggccg ctcagcgtct gccgggcacg
     8521 atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt tttgcatatg
     8581 ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag
     8641 acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc gatgctgact
     8701 attgatatgc cgtcggtggg cttttcatca aaatggaagc tcacccagga ctccagcctg
     8761 ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc
     8821 gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata tcttgaatcg
     8881 ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt gagtgatttt
     8941 aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg tttggggatg
     9001 catgatgctt ccgacgaagc gttgcgcgtg gagctgaatc gctattcatt aaaagtgcaa
     9061 ggattgctgg gacgtcgctg cccaacgcca atgttatcag gctactggaa gaacgatccg
     9121 ttcagcccgg aagaggactc acgcttaatc acctcatcat ctgctgacgg taaattatta
     9181 gagatcccat ttaacccggt gtatcggaat tttgacaaag gtcttcagga aatcaccgac
     9241 tggatcgaaa aacgcttgtg ttaaaaattt gctaaatttt gccaatttgg taaaacagtt
     9301 gcatcacaac aggagatagc aatgacgtta ccgagtggac acccgaagag cagattgatc
     9361 aaaaaattta ccgcactagg cccgtatatt cgtgaaggta agtgtgaaga taatcgattc
     9421 tttttcgatt gtctggctgt atgcgtcaac gtgaaaccgg caccggaagt gcgtgagttc
     9481 tggggctggt ggatggagct tgaagcgcag gaatcccgtt ttacctacag ttaccagttt
     9541 ggtctgttcg ataaagcagg cgactggaag agcgttccgg taaaagacac tgaagtggtt
     9601 gaacgactgg agcacaccct gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg
     9661 ctgaatctga agctggaacc ggcggatgat tttcgtgacg agccggtgaa gttaacggcg
     9721 tgagtgaaat gtgccggatg catcacatcc ggcaataatc attaaaactg ataggtcatg
     9781 ccaaccgcga caatatcatc attattaata ttcaatttgt tatcgctatc cagttggttg
     9841 attttataat caacaaacgc tgacatattt ttgttgaaat aatatgtagc cccgacatcg
     9901 atataattga ccagatcttc atcaccgata ccttcaatat ctttcccttt cgataagaca
     9961 taacccagcg atggacgcag accaaagtca aactggtatt gagcgaccgc ttcaaagttc
    10021 tgtgtcttat tggcaaagcc gccagttatt ggcgtcattt tgcgtgtttc agaataaaaa
    10081 gttgccagat aaatattatt ggcatcgtat ttcagacctg tagcccatgc ttctgcacgc
    10141 ttgcctgtgc cacggctttg caggttctgc tcgttggtgc gatctgagtt ggtataggcc
    10201 ccactaatgg cgaaatcgct gccgccaaaa tcatatgtca atgacgtgcc gaagccatcg
    10261 ccgttttgct ttttaacgtc gcggttttcg tttttccctt gatattgcag ggttaagttc
    10321 aggccatcga taacgccgaa gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt
    10381 ttggtcataa agttgtcggt ctgcgccgag gagtcgccac caaattccgg gaacatatcg
    10441 gtccaggctt ccacgtcata caacgcgccc aggttacgac cataatcgaa agaacccaaa
    10501 tctttatatt tcaacccggc aaaagcgaga cgcgtttttt gctgtgcagt atcactctct
    10561 gctttattac cggcaaactc tgcttcccaa cgaccataac cagtcagttg atcgttaatt
    10621 tgtgtttcgc ctttaaaacc aaaacggata taactctggt cgccatcttt actgtcgtta
    10681 tcactcatat aatgcatggc tttaactttg ccatagacat ccagtttatt accgtctttg
    10741 ttatatattt ctgcggcctg tacggatgca gatgccacaa tgcccatcac cactaatgcc
    10801 agagtgctct ttttcatttt cattcctgat tttaattaac gcgcgaatat tctgcgggag
    10861 agcccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat tacagattaa
    10921 tgatattctt aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc
    10981 taggcttgct gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg
    11041 acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga
    11101 atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga
    11161 tcgcgccgcc tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat
    11221 gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac
    11281 ctgggttacc cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg
    11341 cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg
    11401 caaatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac
    11461 accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc
    11521 gctacggcag agattaaagt cggcgataac gacaaccttt ctgcactggc ggcgattctg
    11581 gcgggtgccg ataaactgtt gttactgacc gatcaaaaag gtttgtatac cgctgacccg
    11641 cgcagtaatc cgcaggcaga attgataaaa gatgtttacg gtattgatga cgcactgcgc
    11701 gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac caaattgcag
    11761 gccgcggacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg
    11821 ggcgtcattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg
    11881 actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg
    11941 gtagatgaag gggcaaccgc cgccattctt gaacgcggca gctccctgtt gccgaaaggc
    12001 attaaaagcg tgactggcaa cttctcgcgt ggtgaagtca tccgcatttg taacctcgaa
    12061 ggtcgcgata tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg ccgtattgcc
    12121 ggacaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt
    12181 caccgtgatg acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg
    12241 ccgcgaagca agcctcgtat aaattagcgc aactctcctg ccgcgaaaaa aatcgcgtgc
    12301 tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc
    12361 aggatgttgc tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac
    12421 tgacgcccgc acggctgaaa ggcattgccg acgatgtgcg ccaggtgtgt aacctcgccg
    12481 atccggtggg gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc
    12541 gtcgcgtacc gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg
    12601 atgttgcttc actgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggcaaagaaa
    12661 cctgtcgcac taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct
    12721 taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc
    12781 tgcgtatgga taaatacatc gacatgctga tcccgcgcgg cggggctggt ttgcataaac
    12841 tgtgccgcga acagtcgacg atcccggtga tcacaggtgg tataggcgta tgccatattt
    12901 acgttgatga aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc
    12961 agcgtccgag cacatgtaat acggtagaaa cgttgctggt gaataaaaac atcgccgata
    13021 gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg
    13081 cagcagcgct gacacagttg caggcaggcc ccgcgaaggt ggtggcggtt aaagccgaag
    13141 agtatgacga tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg
    13201 atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg
    13261 atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg
    13321 cctctacgcg ttttaccgac ggcggccagt ttggactggg tgcggaagtg gcggtaagca
    13381 cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc gctgaccact tacaagtgga
    13441 tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca
    13501 tttgattcac aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt
    13561 cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc
    13621 gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac catttaaatc aagtagttac
    13681 cccatattca aatacaccac gtttcctcct gtgccgtatt tatgccattg taaccctggc
    13741 aattaatcaa aatactgttc tgacatcagg caatgcaggt gcagacattt aagccaattg
    13801 ctgccgccat tccttgacgt ggtcaatcag ggcgcggagc tttggtgcaa tattgcgacg
    13861 ctgtgggaaa tacagataga agcccggaaa ttgtggaaga aagtcatcaa gcagagatac
    13921 aagcttaccg ctttcaatat acggcctgaa agtttcctga gtggcaattg ttattcctcc
    13981 gccggcaaga gccagcctca acatcagacg cagatcatta gtcgtaatct gtggttcaat
    14041 cgcaaggtcg aaagctctcc cgttttcttc aaatggccag cgataaggcg caacatccgg
    14101 ggattgacgc cagccgatac acttatgatt cacaagttca cgcggatgaa caggtgtgct
    14161 gttggctgcg agataggagg gagaggcaac gaccatttca cgctgcttac cggtaagggg
    14221 aacagcaatc atgtcttttt caatgacctc tccgagcctt acgccagcat cgtaacctgc
    14281 ggcaacgata tcgaattcct catccgtgac aacaatatca agcgtaacgg ctggattggc
    14341 agctgcaaac gaagcgatta gcggacctga aagaaattct tctgctatcg acgtcaccgc
    14401 gattctgaga tgtccacgtg gtacatggtc agagaccatc tcttcgaacg ctgcctcaat
    14461 accggataaa ggtaatgaca acgatttatg cagccgttct cccgcttccg tcaaattaac
    14521 tgagcgcgtg gtccgcatta caagcattgt cccgaatgca tcttcaagac gtcttattcc
    14581 ctgactgacc gctgaacggg taacgcccag tcgtgcggcg gctttactga aattactctc
    14641 ttctgctacc gcgataaaaa tgggtaaaag attaagatcg attttcattg tttaatgccg
    14701 ctaaccactg agtacattga aagcaggata ctggaaccaa tcataaagtt ctattctctc
    14761 tccgaacaca tttgaaggag aacgtaatgg ataaggtcat attaatcacc ggcgcatcaa
    14821 gtggtattgg ggaaggtatt gctagagagc tgggaacgac aggtgcaaag actttactgg
    14881 gagcacgcag agttgagcgt atcgaagcca ttgcaaccga aatttgccgc gcaggaggaa
    14941 ttgctaaagc tcgggagttg gatgtcacag accgacagtc catggcagat ttcgtgcaag
    15001 tagcgctgga tagctggggg cgagttgatg ttcttatcaa taatgcgggc gttatgccgc
    15061 tttcaccgct tgcagcagga aaacagaatg aatgggagct cactattgac gtgaatatca
    15121 aaggtgtact gtggggaatt ggcgctgtac ttccggtgat ggaagcacag ggttccggtc
    15181 agataatcaa tcttggttct attggtgccc tttctgttgt gcccacaggc gcagtctatt
    15241 gtgcatcaaa atttgcagta cgggctattt ctgatggctt aaggcaggaa agctcgaaaa
    15301 tccgtgtgac ctgcgtaaat cctggcgttg tcgaaagtga actggcttca accattaccc
    15361 atgtggaaac catgaaggca atggacgcct accgttctgt tgcacttcaa cctgcggata
    15421 ttgcccgtgc ggtaaggcat attattgaat ctcccgaaag cgttgataca accgaaatta
    15481 ccatcagacc cactgcatct gcaaactgaa gaataatttt tacattcacg ttttattaaa
    15541 tatgtcagaa gcaaaattta tatcaacctg ttttgcatag caagatgcat tatgtattgc
    15601 tcaccccgaa ggagatgagg gtttaattac agaaggagtg ttttctcttg gggtaaatat
    15661 gactcatgtt taatacctat gtatataaaa ctcatcctct caat
//
LOCUS       DAPGRN010000401         5467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.212, whole genome shotgun sequence.
ACCESSION   DAPGRN010000401 DAPGRN010000000
VERSION     DAPGRN010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.212"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..1222)
                     /locus_tag="RHW33_005022"
     CDS             complement(14..1222)
                     /locus_tag="RHW33_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDV1648408.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            1508..2311
                     /locus_tag="RHW33_005023"
                     /note="aph(3'')-Ib"
     CDS             1508..2311
                     /locus_tag="RHW33_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV1648409.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2311..3147
                     /locus_tag="RHW33_005024"
                     /pseudo
     CDS             2311..3147
                     /locus_tag="RHW33_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
     gene            3483..4298
                     /locus_tag="RHW33_005025"
     CDS             3483..4298
                     /locus_tag="RHW33_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV1648410.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4452..5327)
                     /locus_tag="RHW33_005026"
     CDS             complement(4452..5327)
                     /locus_tag="RHW33_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV1648411.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
BASE COUNT         1274 a         1333 c         1446 g         1414 t
ORIGIN      
        1 ctgagagatc ccctcataat ttccccaaaa cgtaaccatg tgtgaataga ttttgagtaa
       61 gcagggttgc agccacgagt gagtcttccc ttgttattgt gtagccagaa tgccgcaaaa
      121 cttccatgcc taagcgaact gttgagagta cgtttcgatt tctgactgtg ttagcctgga
      181 agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc aagccaacat gttagttgaa
      241 gcatcagggc gattagcagc atgatatcaa aacgctctga gctgctcgtt cggctatggc
      301 gtaggcctag tccgtaggca ggacttttca agtctcggaa ggtttcttca atctgcattc
      361 gcttcgaata gatattaaca agttgtttgg gtgttcgaat ttcaacaggt aagttagttg
      421 ctagaatcca tggctccttt gccgacgctg agtagatttt aggtgacggg tggtgacaat
      481 gagtccgtgt cgagcgctga ttttttcggc ctttagagcg agatttatac aatagaattt
      541 ggcatgagat tggattgctt ttagtcagcc tcttatagcc taaagtcttt gagtgactag
      601 atgacatatc atgtaagttg ctgataggtt tccagttttc cgctcctagg tctgcatatt
      661 gtacttttcc tcttactcga cttaaccagt accaacccag cttctcaacg gatttatacc
      721 atggcacttt aaagccagca tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc
      781 tcgcaaggtc ggctagaaat tggtcatgag ctttctttga acattgctct gaaagcggga
      841 acgctttctc ataaagagta acagaacgac cgtgtagtgc gactgaagct ctcaatacca
      901 taagccgttt ttgctcacgg atatcagacc agtcaacaag tacaatgggc atcgtattgc
      961 ccgaacagat aaagctagca tgccaacggt atacagcgag tcgctctttg tggaggtgac
     1021 gattacctaa caatcggtcg attcgtttga tgttatgttt tgttctcgct ttggttggca
     1081 ggttacggcc aagttcggta agagtgagag ttttacagtc aagtaaggcg tggcaagcca
     1141 acgttaagct gttgagtcgt tttaagtgta attcggggca gaattggtaa agagagtcgt
     1201 gtaaaatatc gagttcgcac attttgttgt ctgattattg atttttggcg aaaccatttg
     1261 atcatatgac aagatgtgta tctaccttaa cttaatgatt ttgataaaaa tcattagggg
     1321 attcatcagc actcggctgg aaatcgccgg tggcatttga gaaaaaagcc gcttaaaatg
     1381 agagatagac cggaacacaa ccggtgcaag accatggaaa gcatgttctc ggacttacgt
     1441 agcaactcgt ttcttttcgc aggttgagcc acctccgcgc ttcatcagaa aactgaagga
     1501 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1561 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1621 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1681 caaaggtcga ggtgtggctt gccccgaggt gatcaactgg caggaggaac aggagggtgc
     1741 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     1801 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     1861 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     1921 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     1981 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     2041 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     2101 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     2161 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     2221 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     2281 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
     2341 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagta
     2401 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     2461 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     2521 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     2581 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     2641 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     2701 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgacaaac tgactacgtc
     2761 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     2821 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     2881 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     2941 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     3001 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc
     3061 gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc
     3121 aagcaggtgc gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca
     3181 catcaatctt accggagaat atcgttggcc aaagccttag cgtaggattt cgccctctcc
     3241 cgcaaacgac ccctttattg taggtgggcc agttggtgat tttgaacttt tgctttgcca
     3301 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     3361 gatttattca acaaatccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     3421 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     3481 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacctgg
     3541 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     3601 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     3661 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gcatttatgc
     3721 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3781 cgctccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     3841 ttgttgatgc gctggcagcg ttcctgcgcc ggttgcattc gattcctgtt agtaattgtc
     3901 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     3961 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     4021 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4081 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4141 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgaatttt
     4201 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4261 aattgcagtt tcatttgatg ctcgatgagt ttttctgata gttagtcttt gtagagacac
     4321 aaacctgaaa ttccggacac cgcgcttcag gacatctccc tggacgccga tatctggcag
     4381 tatccggacg gtactatcga acgccgggcc aacggtactc ccctgccctt tatgactgac
     4441 gaccggctat ttcacccacg cacttctcca tgaaatttag catcgtcgcg gttaatgtcg
     4501 aaaatttgcc acctgacgtg gttggcatcg agggtgaaaa ctgccgtggc gagggtgttc
     4561 tcctcatccg gatcgtcggc agccagccgg taaattggca gttctgcgtc atgggtatct
     4621 gagagaatgg tttttgccgc cgcggcgtca aacggaggtt gcgtttcccg ccactgcacg
     4681 aggcgtaact gccgcgacgc tgaactgtcg gtaacgatct gctcaacgcc agccagtgcc
     4741 gcatgcacca gatggttggc atgcccggtt accgtcgtca ggggtagaac ggaacagcct
     4801 tgaccggtgg cctcaacgct gaacagccgc gcatcgccca gctgcccgag cgtatggtga
     4861 aacgccccgg atcgcggctg gccggtaagt atagtgatgg cctcgtcgag ggtgttcgca
     4921 ttcagcgagg cgcgagccag gatctgacgc ggtaatccct gcggacgata cactgcacgg
     4981 atgttattca ccgtgttaac gatacctttt tcatttaccg caaaggtgtg gccgcaaatc
     5041 gaccccggat aggcaaaact cgtaaagctc agaccgcgac tcggagtaat ggtagccagc
     5101 aagcattcat cccgtaactg aggaaaaccg tcttcattat gcgcgataat ccgctcgcct
     5161 gcggcgttta ccccggccac cgtcgtacag ccatccgacg ttgagcgcac cagatcgcca
     5221 cggcagttcc aggcaaagac ctgatcaaca gtggcctcca gtccctgcgc cagcccctcc
     5281 agctcctgcc agatgtgcgg ataatgccgc tgcacctcgg cgcgcattaa ccgggtctca
     5341 ggggatgcct gcagggtcat cacggggtcg tttgcgggag ggggcggaat cctacgctaa
     5401 ggctttggcc agcgatattc tccggtgaga ttgatgtgtt cccatccgag cggcgaaaca
     5461 tgggcca
//
LOCUS       DAPGRN010000402         5467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.213, whole genome shotgun sequence.
ACCESSION   DAPGRN010000402 DAPGRN010000000
VERSION     DAPGRN010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.213"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..1222)
                     /locus_tag="RHW33_005027"
     CDS             complement(14..1222)
                     /locus_tag="RHW33_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDV1648412.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            1508..2311
                     /locus_tag="RHW33_005028"
                     /note="aph(3'')-Ib"
     CDS             1508..2311
                     /locus_tag="RHW33_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV1648413.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2311..3147
                     /locus_tag="RHW33_005029"
     CDS             2311..3147
                     /locus_tag="RHW33_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV1648414.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3483..4298
                     /locus_tag="RHW33_005030"
     CDS             3483..4298
                     /locus_tag="RHW33_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV1648415.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(4452..5327)
                     /locus_tag="RHW33_005031"
     CDS             complement(4452..5327)
                     /locus_tag="RHW33_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV1648416.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
BASE COUNT         1272 a         1333 c         1446 g         1416 t
ORIGIN      
        1 ctgagagatc ccctcataat ttccccaaaa cgtaaccatg tgtgaataga ttttgagtaa
       61 gcagggttgc agccacgagt gagtcttccc ttgttattgt gtagccagaa tgccgcaaaa
      121 cttccatgcc taagcgaact gttgagagta cgtttcgatt tctgactgtg ttagcctgga
      181 agtgcttgtc ccaaccttgt ttctgagcat gaacgcccgc aagccaacat gttagttgaa
      241 gcatcagggc gattagcagc atgatatcaa aacgctctga gctgctcgtt cggctatggc
      301 gtaggcctag tccgtaggca ggacttttca agtctcggaa ggtttcttca atctgcattc
      361 gcttcgaata gatattaaca agttgtttgg gtgttcgaat ttcaacaggt aagttagttg
      421 ctagaatcca tggctccttt gccgacgctg agtagatttt aggtgacggg tggtgacaat
      481 gagtccgtgt cgagcgctga ttttttcggc ctttagagcg agatttatac aatagaattt
      541 ggcatgagat tggattgctt ttagtcagcc tcttatagcc taaagtcttt gagtgactag
      601 atgacatatc atgtaagttg ctgataggtt tccagttttc cgctcctagg tctgcatatt
      661 gtacttttcc tcttactcga cttaaccagt accaacccag cttctcaacg gatttatacc
      721 atggcacttt aaagccagca tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc
      781 tcgcaaggtc ggctagaaat tggtcatgag ctttctttga acattgctct gaaagcggga
      841 acgctttctc ataaagagta acagaacgac cgtgtagtgc gactgaagct ctcaatacca
      901 taagccgttt ttgctcacgg atatcagacc agtcaacaag tacaatgggc atcgtattgc
      961 ccgaacagat aaagctagca tgccaacggt atacagcgag tcgctctttg tggaggtgac
     1021 gattacctaa caatcggtcg attcgtttga tgttatgttt tgttctcgct ttggttggca
     1081 ggttacggcc aagttcggta agagtgagag ttttacagtc aagtaaggcg tggcaagcca
     1141 acgttaagct gttgagtcgt tttaagtgta attcggggca gaattggtaa agagagtcgt
     1201 gtaaaatatc gagttcgcac attttgttgt ctgattattg atttttggcg aaaccatttg
     1261 atcatatgac aagatgtgta tctaccttaa cttaatgatt ttgataaaaa tcattagggg
     1321 attcatcagc actcggctgg aaatcgccgg tggcatttga gaaaaaagcc gcttaaaatg
     1381 agagatagac cggaacacaa ccggtgcaag accatggaaa gcatgttctc ggacttacgt
     1441 agcaactcgt ttcttttcgc aggttgagcc acctccgcgc ttcatcagaa aactgaagga
     1501 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1561 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1621 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1681 caaaggtcga ggtgtggctt gccccgaggt gatcaactgg caggaggaac aggagggtgc
     1741 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     1801 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     1861 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     1921 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     1981 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     2041 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     2101 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     2161 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     2221 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     2281 tctgcgattg gaccctctga cttggggttg atgttcatgc cgcctgtttt tcctgctcat
     2341 tggcacgttt cgcaacctgt tctcattgcg gacacctttt ccagcctcgt ttggaaagtt
     2401 tcattgccag acgggactcc tgcaatcgtc aagggattga aacctataga agacattgct
     2461 gatgaactgc gcggggccga ctatctggta tggcgcaatg ggaggggagc agtccggttg
     2521 ctcggtcgtg agaacaatct gatgttgctc gaatatgccg gggagcgaat gctctctcac
     2581 atcgttgccg agcacggcga ctaccaggcg accgaaattg cagcggaact aatggcgaag
     2641 ctgtatgccg catctgagga acccctgcct tctgcccttc tcccgatccg ggatcgcttt
     2701 gcagctttgt ttcagcgggc gcgcgatgat caaaacgcag gttgtcaaac tgactacgtc
     2761 cacgcggcga ttatagccga tcaaatgatg agcaatgcct cggaactgcg tgggctacat
     2821 ggcgatctgc atcatgaaaa catcatgttc tccagtcgcg gctggctggt gatagatccc
     2881 gtcggtctgg tcggtgaagt gggctttggc gccgccaata tgttctacga tccggctgac
     2941 agagacgacc tttgtctcga tcctagacgc attgcacaga tggcggacgc attctctcgt
     3001 gcgctggacg tcgatccgcg tcgcctgctc gaccaggcgt acgcttatgg gtgcctttcc
     3061 gcagcttgga acgcggatgg agaagaggag caacgcgatc tagctatcgc ggccgcgatc
     3121 aagcaggtgc gacagacgtc atactagata tcaagcgact tctcctatcc cctgggaaca
     3181 catcaatctt accggagaat atcgttggcc aaagccttag cgtaggattt cgccctctcc
     3241 cgcaaacgac ccctttattg taggtgggcc agttggtgat tttgaacttt tgctttgcca
     3301 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     3361 gatttattca acaaatccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     3421 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     3481 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacctgg
     3541 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     3601 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     3661 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gcatttatgc
     3721 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3781 cgctccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     3841 ttgttgatgc gctggcagcg ttcctgcgcc ggttgcattc gattcctgtt agtaattgtc
     3901 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     3961 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     4021 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     4081 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     4141 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgaatttt
     4201 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     4261 aattgcagtt tcatttgatg ctcgatgagt ttttctgata gttagtcttt gtagagacac
     4321 aaacctgaaa ttccggacac cgcgcttcag gacatctccc tggacgccga tatctggcag
     4381 tatccggacg gtactatcga acgccgggcc aacggtactc ccctgccctt tatgactgac
     4441 gaccggctat ttcacccacg cacttctcca tgaaatttag catcgtcgcg gttaatgtcg
     4501 aaaatttgcc acctgacgtg gttggcatcg agggtgaaaa ctgccgtggc gagggtgttc
     4561 tcctcatccg gatcgtcggc agccagccgg taaattggca gttctgcgtc atgggtatct
     4621 gagagaatgg tttttgccgc cgcggcgtca aacggaggtt gcgtttcccg ccactgcacg
     4681 aggcgtaact gccgcgacgc tgaactgtcg gtaacgatct gctcaacgcc agccagtgcc
     4741 gcatgcacca gatggttggc atgcccggtt accgtcgtca ggggtagaac ggaacagcct
     4801 tgaccggtgg cctcaacgct gaacagccgc gcatcgccca gctgcccgag cgtatggtga
     4861 aacgccccgg atcgcggctg gccggtaagt atagtgatgg cctcgtcgag ggtgttcgca
     4921 ttcagcgagg cgcgagccag gatctgacgc ggtaatccct gcggacgata cactgcacgg
     4981 atgttattca ccgtgttaac gatacctttt tcatttaccg caaaggtgtg gccgcaaatc
     5041 gaccccggat aggcaaaact cgtaaagctc agaccgcgac tcggagtaat ggtagccagc
     5101 aagcattcat cccgtaactg aggaaaaccg tcttcattat gcgcgataat ccgctcgcct
     5161 gcggcgttta ccccggccac cgtcgtacag ccatccgacg ttgagcgcac cagatcgcca
     5221 cggcagttcc aggcaaagac ctgatcaaca gtggcctcca gtccctgcgc cagcccctcc
     5281 agctcctgcc agatgtgcgg ataatgccgc tgcacctcgg cgcgcattaa ccgggtctca
     5341 ggggatgcct gcagggtcat cacggggtcg tttgcgggag ggggcggaat cctacgctaa
     5401 ggctttggcc agcgatattc tccggtgaga ttgatgtgtt cccatccgag cggcgaaaca
     5461 tgggcca
//
LOCUS       DAPGRN010000403         2611 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.248, whole genome shotgun sequence.
ACCESSION   DAPGRN010000403 DAPGRN010000000
VERSION     DAPGRN010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.248"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            44..1189
                     /locus_tag="RHW33_005032"
     CDS             44..1189
                     /locus_tag="RHW33_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV1648417.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1283..1816
                     /locus_tag="RHW33_005033"
     CDS             1283..1816
                     /locus_tag="RHW33_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV1648418.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(1813..2130)
                     /gene="sugE"
                     /locus_tag="RHW33_005034"
     CDS             complement(1813..2130)
                     /gene="sugE"
                     /locus_tag="RHW33_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV1648419.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
BASE COUNT          684 a          673 c          658 g          596 t
ORIGIN      
        1 cgggcccgga cacctttttg cttttaatta cggaactgat ttcatgatga aaaaatcgtt
       61 atgctgcgct ctgctgctga cagcctcttt ctccacattt gctgccgcaa aaacagaaca
      121 acagattgcc gatatcgtta atcgcaccat caccccgttg atgcaggagc aggctattcc
      181 gggtatggcc gttgccgtta tctaccaggg aaaaccctat tatttcacct ggggtaaagc
      241 cgatatcgcc aataaccacc cagtcacgca gcaaacgctg tttgagctag gatcggttag
      301 taagacgttt aacggcgtgt tgggcggcga tgctatcgcc cgcggcgaaa ttaagctcag
      361 cgatccggtc acgaaatact ggccagaact gacaggcaaa cagtggcagg gtatccgcct
      421 gctgcactta gccacctata cggcaggcgg cctaccgctg cagatccccg atgacgttag
      481 ggataaagcc gcattactgc atttttatca aaactggcag ccgcaatgga ctccgggcgc
      541 taagcgactt tacgctaact ccagcattgg tctgtttggc gcgctggcgg tgaaaccctc
      601 aggaatgagt tacgaagagg caatgaccag acgcgtcctg caaccattaa aactggcgca
      661 tacctggatt acggttccgc agaacgaaca aaaagattat gcctggggct atcgcgaagg
      721 gaagcccgta cacgtttctc cgggacaact tgacgccgaa gcctatggcg tgaaatccag
      781 cgttattgat atggcccgct gggttcaggc caacatggat gccagccacg ttcaggagaa
      841 aacgctccag cagggcattg cgcttgcgca gtctcgctac tggcgtattg gcgatatgta
      901 ccagggatta ggctgggaga tgctgaactg gccgctgaaa gctgattcga tcatcaacgg
      961 cagcgacagc aaagtggcat tggcagcgct tcccgccgtt gaggtaaacc cgcccgcccc
     1021 cgcagtgaaa gcctcatggg tgcataaaac gggctccact ggtggatttg gcagctacgt
     1081 agccttcgtt ccagaaaaaa accttggcat cgtgatgctg gcaaacaaaa gctatcctaa
     1141 ccctgtccgt gtcgaggcgg cctggcgcat tcttgaaaag ctgcaataac tgacgatgag
     1201 gcccaggata ttgggcctcc tttctttctc tttttttcct gttgtcatct acacttaaca
     1261 aaaatacagc aaggaaaatc ccatgcgcat tttgcccgtc gttgctgcag ttacggctgc
     1321 attcctggtt gtcgcgtgta gctccccgac accgccgaaa ggcgttaccg tggtaaataa
     1381 ctttgatgcc aaacgctatc tgggaacctg gtatgaaatt gcgcgcttcg accatcgttt
     1441 cgagcgcgga ttggataaag tgaccgcaac atacagcttg cgcgacgacg gcggcatcaa
     1501 cgttattaac aagggctata accctgacag ggagatgtgg cagaaaacgg aagggaaagc
     1561 ctatttcacc ggcgacccaa gcagagccgc gcttaaggtt tcttttttcg gccccttcta
     1621 tggcgggtat aacgtaattg cactcgaccg ggaatatcgt cacgcgctgg tttgtggtcc
     1681 ggatcgcgac tacctgtgga tcctttcacg gacccctact atttcagatg aaatgaaaca
     1741 gcaaatgtta gccatcgcga cccgggaagg gtttgaagtg aataaactga tttgggtgaa
     1801 acagcctggc gcttagtgag tgctcagctt cagaccaata atgccagcaa cgatcagccc
     1861 aaggctcagc aaacgtgccg ggctggcaga ctcacccagc agcaaaatcc ctgtaatggc
     1921 cgccccaaca gcgccaatac cggtccagac cgcataagcg gttcctacag gcaacgtgcg
     1981 cattgcccaa gagagcatgg cgatactgac gatcatcgcc gcaatagtga taatgcttgg
     2041 cgtaagacgc gtaaaaccgt gggtgtattt caggccaatc gcccagacaa cttcgagcaa
     2101 acctgcaatt aataaaacga tccaggacat atcaggctcc agaacaatgg ggccgtcccc
     2161 ggtgaaagaa gcgtttgcag gtcgtcctgc aaagctaatg tgtgaaatgg catttttgcc
     2221 cggaagaaaa tgaatttcaa cctttttatt caccgcctgc taaaagcaag aattaagcat
     2281 aattagcggc gtagttcccg catttggcac tcatcggagt tctctatgat gaagggcttt
     2341 ctgaaaccag aaagtcgatt gcccctcttc tgaatgcgaa acgtttatgc ttaaaatttt
     2401 agtgattgac cggtgccact ttacccgcac agggatagag taataatgac acataatcat
     2461 tgtttatcat ttctggccag agataggctc ggcaagaaat tcaaaccggc gtaccttgat
     2521 gctctgatca tgcattacga tggtacccgg ctcatacttg acggaaaata caacaaagag
     2581 gcatagagga tatttgaatt tacataaatt c
//
LOCUS       DAPGRN010000404         1659 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC10824
            SAMN37210923-rid19354613.guided.275, whole genome shotgun sequence.
ACCESSION   DAPGRN010000404 DAPGRN010000000
VERSION     DAPGRN010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37210923
            Sequence Read Archive: SRR25825085
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875809.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875809.1
            Genome Coverage       :: 70x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 06:34:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,036
            CDSs (total)                      :: 4,957
            Genes (coding)                    :: 4,861
            CDSs (with protein)               :: 4,861
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 34 of 96
            Pseudo Genes (incomplete)         :: 56 of 96
            Pseudo Genes (internal stop)      :: 21 of 96
            Pseudo Genes (multiple problems)  :: 12 of 96
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37210923-rid19354613.guided.275"
                     /isolate="ARS-CC10824"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..1262
                     /gene="tet(B)"
                     /locus_tag="RHW33_005035"
     CDS             57..1262
                     /gene="tet(B)"
                     /locus_tag="RHW33_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV1648420.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1375..>1659)
                     /locus_tag="RHW33_005036"
     CDS             complement(1375..>1659)
                     /locus_tag="RHW33_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV1648421.1"
                     /translation="NKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQL
                     KAYDDDVLKNLLNGWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          412 a          305 c          370 g          572 t
ORIGIN      
        1 ctctatcatt gatagagtta ttttaccact ccctatcagt gatagagaaa agtgaaatga
       61 atagttcgac aaagatcgca ttggtaatta cgttattcga tgccatgggg attggcctta
      121 tcatgccagt cttgccaacg ttattacgtg aatttattgc ttcggaagat atcgctaacc
      181 actttggcgt attgcttgca ctttatgcgt taatgcaggt tatctttgct ccttggcttg
      241 gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt gttgtcatta ataggcgcat
      301 cgctggatta cttattgctg gctttttcaa gtgcgctttg gatgctgtat ttaggccgtt
      361 tgctttcagg gatcacagga gctactgggg ctgtcgcggc atcggtcatt gccgatacca
      421 cctcagcttc tcaacgcgtg aagtggttcg gttggttagg ggcaagtttt gggcttggtt
      481 taatagcggg gcctattatt ggtggttttg caggagagat ttcaccgcat agtccctttt
      541 ttatcgctgc gttgctaaat attgtcactt tccttgtggt tatgttttgg ttccgtgaaa
      601 ccaaaaatac acgtgataat acagataccg aagtaggggt tgagacgcaa tcgaattcgg
      661 tatacatcac tttatttaaa acgatgccca ttttgttgat tatttatttt tcagcgcaat
      721 tgataggcca aattcccgca acggtgtggg tgctatttac cgaaaatcgt tttggatgga
      781 atagcatgat ggttggcttt tcattagcgg gtcttggtct tttacactca gtattccaag
      841 cctttgtggc aggaagaata gccactaaat ggggcgaaaa aacggcagta ctgctcggat
      901 ttattgcaga tagtagtgca tttgcctttt tagcgtttat atctgaaggt tggttagttt
      961 tccctgtttt aattttattg gctggtggtg ggatcgcttt acctgcatta cagggagtga
     1021 tgtctatcca aacaaagagt catcagcaag gtgctttaca gggattattg gtgagcctta
     1081 ccaatgcaac cggtgttatt ggcccattac tgtttgctgt tatttataat cattcactac
     1141 caatttggga tggctggatt tggattattg gtttagcgtt ttactgtatt attatcctgc
     1201 tatcgatgac cttcatgtta acccctcaag ctcaggggag taaacaggag acaagtgctt
     1261 agttatttcg tcaccaaatg atgttattcc gcgaaatata atgaccctct tgataaccca
     1321 agagggcatt ttttacgata aagaagattt agcttcaaat aaaacctatc tattttattt
     1381 atctttcaag ctcaataaaa agccgcggta aatagcaata aattggcctt ttttatcggc
     1441 aagctctttt aggtttttcg catgtattgc gatatgcata aaccagccat tgagtaagtt
     1501 tttaagcaca tcatcatcat aagctttaag ttggttctct tggatcaatt tgctgacaat
     1561 ggcgtttacc ttaccagtaa tgtattcaag gctaattttt tcaagttcat tccaaccaat
     1621 gataggcatc acttcttgga tagggataag gtttttatt
//
